LOCUS       DAZKGS010000001       114766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKGS010000001 DAZKGS010000000
VERSION     DAZKGS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 114766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..461
                     /locus_tag="ACHOQY_000001"
     CDS             <1..461
                     /locus_tag="ACHOQY_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9676174.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRG
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            830..3676
                     /locus_tag="ACHOQY_000002"
     CDS             830..3676
                     /locus_tag="ACHOQY_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFP9676175.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGEPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            4004..4822
                     /locus_tag="ACHOQY_000003"
     CDS             4004..4822
                     /locus_tag="ACHOQY_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9676176.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4914..5399
                     /locus_tag="ACHOQY_000004"
     CDS             4914..5399
                     /locus_tag="ACHOQY_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9676177.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5415..5891
                     /locus_tag="ACHOQY_000005"
     CDS             5415..5891
                     /locus_tag="ACHOQY_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9676178.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            5954..6175
                     /locus_tag="ACHOQY_000006"
     CDS             5954..6175
                     /locus_tag="ACHOQY_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9676179.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6249..6656
                     /locus_tag="ACHOQY_000007"
     CDS             6249..6656
                     /locus_tag="ACHOQY_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9676180.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(7197..8444)
                     /locus_tag="ACHOQY_000008"
     CDS             complement(7197..8444)
                     /locus_tag="ACHOQY_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP9676181.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(8516..9430)
                     /locus_tag="ACHOQY_000009"
     CDS             complement(8516..9430)
                     /locus_tag="ACHOQY_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP9676182.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            9646..11079
                     /gene="cscA"
                     /locus_tag="ACHOQY_000010"
     CDS             9646..11079
                     /gene="cscA"
                     /locus_tag="ACHOQY_000010"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9676183.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(11087..12082)
                     /locus_tag="ACHOQY_000011"
     CDS             complement(11087..12082)
                     /locus_tag="ACHOQY_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9676184.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            12325..12543
                     /locus_tag="ACHOQY_000012"
                     /pseudo
     CDS             12325..12543
                     /locus_tag="ACHOQY_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHOQY_000013"
     CDS             12561..13889
                     /gene="dsdA"
                     /locus_tag="ACHOQY_000013"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9676185.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHOQY_000014"
     CDS             complement(13997..15535)
                     /gene="emrY"
                     /locus_tag="ACHOQY_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9676186.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(15535..16698)
                     /gene="emrK"
                     /locus_tag="ACHOQY_000015"
     CDS             complement(15535..16698)
                     /gene="emrK"
                     /locus_tag="ACHOQY_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9676187.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            17114..17728
                     /gene="evgA"
                     /locus_tag="ACHOQY_000016"
     CDS             17114..17728
                     /gene="evgA"
                     /locus_tag="ACHOQY_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9676188.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            17733..21326
                     /gene="evgS"
                     /locus_tag="ACHOQY_000017"
     CDS             17733..21326
                     /gene="evgS"
                     /locus_tag="ACHOQY_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9676189.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(21382..22527)
                     /gene="yfdE"
                     /locus_tag="ACHOQY_000018"
     CDS             complement(21382..22527)
                     /gene="yfdE"
                     /locus_tag="ACHOQY_000018"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9676190.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(22601..23545)
                     /gene="yfdV"
                     /locus_tag="ACHOQY_000019"
     CDS             complement(22601..23545)
                     /gene="yfdV"
                     /locus_tag="ACHOQY_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9676191.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(23615..25309)
                     /gene="oxc"
                     /locus_tag="ACHOQY_000020"
     CDS             complement(23615..25309)
                     /gene="oxc"
                     /locus_tag="ACHOQY_000020"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9676192.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(25363..26613)
                     /gene="frc"
                     /locus_tag="ACHOQY_000021"
     CDS             complement(25363..26613)
                     /gene="frc"
                     /locus_tag="ACHOQY_000021"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9676193.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(27126..27758)
                     /gene="yfdX"
                     /locus_tag="ACHOQY_000022"
     CDS             complement(27126..27758)
                     /gene="yfdX"
                     /locus_tag="ACHOQY_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9676194.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            28054..28329
                     /gene="ypdI"
                     /locus_tag="ACHOQY_000023"
     CDS             28054..28329
                     /gene="ypdI"
                     /locus_tag="ACHOQY_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP9676195.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(28406..28648)
                     /gene="yfdY"
                     /locus_tag="ACHOQY_000024"
     CDS             complement(28406..28648)
                     /gene="yfdY"
                     /locus_tag="ACHOQY_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9676196.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            29001..29921
                     /gene="lpxP"
                     /locus_tag="ACHOQY_000025"
     CDS             29001..29921
                     /gene="lpxP"
                     /locus_tag="ACHOQY_000025"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9676197.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            30277..30348
                     /gene="ypdK"
                     /locus_tag="ACHOQY_000026"
     CDS             30277..30348
                     /gene="ypdK"
                     /locus_tag="ACHOQY_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9676198.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(30413..31651)
                     /gene="alaC"
                     /locus_tag="ACHOQY_000027"
     CDS             complement(30413..31651)
                     /gene="alaC"
                     /locus_tag="ACHOQY_000027"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9676199.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            32028..33725
                     /gene="ypdA"
                     /locus_tag="ACHOQY_000028"
     CDS             32028..33725
                     /gene="ypdA"
                     /locus_tag="ACHOQY_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9676200.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            33740..34474
                     /gene="ypdB"
                     /locus_tag="ACHOQY_000029"
     CDS             33740..34474
                     /gene="ypdB"
                     /locus_tag="ACHOQY_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9676201.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            34487..35344
                     /gene="ypdC"
                     /locus_tag="ACHOQY_000030"
     CDS             34487..35344
                     /gene="ypdC"
                     /locus_tag="ACHOQY_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9676202.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(35347..37842)
                     /gene="ptsP"
                     /locus_tag="ACHOQY_000031"
     CDS             complement(35347..37842)
                     /gene="ptsP"
                     /locus_tag="ACHOQY_000031"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9676203.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(37867..38904)
                     /gene="ypdE"
                     /locus_tag="ACHOQY_000032"
     CDS             complement(37867..38904)
                     /gene="ypdE"
                     /locus_tag="ACHOQY_000032"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9676204.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(38904..39989)
                     /gene="ypdF"
                     /locus_tag="ACHOQY_000033"
     CDS             complement(38904..39989)
                     /gene="ypdF"
                     /locus_tag="ACHOQY_000033"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9676205.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(40004..41251)
                     /gene="fryC"
                     /locus_tag="ACHOQY_000034"
     CDS             complement(40004..41251)
                     /gene="fryC"
                     /locus_tag="ACHOQY_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9676206.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(41273..41599)
                     /gene="fryB"
                     /locus_tag="ACHOQY_000035"
     CDS             complement(41273..41599)
                     /gene="fryB"
                     /locus_tag="ACHOQY_000035"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9676207.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(41818..42783)
                     /gene="glk"
                     /locus_tag="ACHOQY_000036"
     CDS             complement(41818..42783)
                     /gene="glk"
                     /locus_tag="ACHOQY_000036"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9676208.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            42987..44243
                     /gene="yfeO"
                     /locus_tag="ACHOQY_000037"
     CDS             42987..44243
                     /gene="yfeO"
                     /locus_tag="ACHOQY_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9676209.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            44358..44684
                     /gene="ypeC"
                     /locus_tag="ACHOQY_000038"
     CDS             44358..44684
                     /gene="ypeC"
                     /locus_tag="ACHOQY_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9676210.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(44825..46063)
                     /gene="mntH"
                     /locus_tag="ACHOQY_000039"
     CDS             complement(44825..46063)
                     /gene="mntH"
                     /locus_tag="ACHOQY_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9676211.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            46399..47601
                     /gene="nupC"
                     /locus_tag="ACHOQY_000040"
     CDS             46399..47601
                     /gene="nupC"
                     /locus_tag="ACHOQY_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9676212.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(47651..49840)
                     /gene="pdeA"
                     /locus_tag="ACHOQY_000041"
     CDS             complement(47651..49840)
                     /gene="pdeA"
                     /locus_tag="ACHOQY_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9676213.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGIFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(50048..50123)
                     /locus_tag="ACHOQY_000042"
     tRNA            complement(50048..50123)
                     /locus_tag="ACHOQY_000042"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50088..50090),aa:Ala,seq:ggc)
     gene            complement(50163..50238)
                     /locus_tag="ACHOQY_000043"
     tRNA            complement(50163..50238)
                     /locus_tag="ACHOQY_000043"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50203..50205),aa:Ala,seq:ggc)
     gene            50459..50818
                     /gene="yfeC"
                     /locus_tag="ACHOQY_000044"
     CDS             50459..50818
                     /gene="yfeC"
                     /locus_tag="ACHOQY_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9676214.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            50820..51212
                     /gene="yfeD"
                     /locus_tag="ACHOQY_000045"
     CDS             50820..51212
                     /gene="yfeD"
                     /locus_tag="ACHOQY_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9676215.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(51264..52679)
                     /gene="gltX"
                     /locus_tag="ACHOQY_000046"
     CDS             complement(51264..52679)
                     /gene="gltX"
                     /locus_tag="ACHOQY_000046"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9676216.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            52938..53013
                     /locus_tag="ACHOQY_000047"
     tRNA            52938..53013
                     /locus_tag="ACHOQY_000047"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52971..52973,aa:Val,seq:tac)
     gene            53058..53133
                     /locus_tag="ACHOQY_000048"
     tRNA            53058..53133
                     /locus_tag="ACHOQY_000048"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53091..53093,aa:Val,seq:tac)
     gene            53138..53213
                     /locus_tag="ACHOQY_000049"
     tRNA            53138..53213
                     /locus_tag="ACHOQY_000049"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53171..53173,aa:Lys,seq:ttt)
     gene            53245..53370
                     /locus_tag="ACHOQY_000050"
     ncRNA           53245..53370
                     /ncRNA_class="other"
                     /locus_tag="ACHOQY_000050"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(53479..54363)
                     /gene="xapR"
                     /locus_tag="ACHOQY_000051"
     CDS             complement(53479..54363)
                     /gene="xapR"
                     /locus_tag="ACHOQY_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP9676217.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(54404..54562)
                     /locus_tag="ACHOQY_000052"
     CDS             complement(54404..54562)
                     /locus_tag="ACHOQY_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP9676218.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(54615..55871)
                     /gene="xapB"
                     /locus_tag="ACHOQY_000053"
     CDS             complement(54615..55871)
                     /gene="xapB"
                     /locus_tag="ACHOQY_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP9676219.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(55931..56764)
                     /gene="xapA"
                     /locus_tag="ACHOQY_000054"
     CDS             complement(55931..56764)
                     /gene="xapA"
                     /locus_tag="ACHOQY_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP9676220.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            57013..57777
                     /gene="yfeN"
                     /locus_tag="ACHOQY_000055"
     CDS             57013..57777
                     /gene="yfeN"
                     /locus_tag="ACHOQY_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP9676221.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(57816..58742)
                     /gene="yfeR"
                     /locus_tag="ACHOQY_000056"
     CDS             complement(57816..58742)
                     /gene="yfeR"
                     /locus_tag="ACHOQY_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9676222.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            58832..59830
                     /gene="yfeH"
                     /locus_tag="ACHOQY_000057"
     CDS             58832..59830
                     /gene="yfeH"
                     /locus_tag="ACHOQY_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9676223.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(59827..60045)
                     /gene="ypeB"
                     /locus_tag="ACHOQY_000058"
     CDS             complement(59827..60045)
                     /gene="ypeB"
                     /locus_tag="ACHOQY_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9676224.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(60047..62062)
                     /gene="ligA"
                     /locus_tag="ACHOQY_000059"
     CDS             complement(60047..62062)
                     /gene="ligA"
                     /locus_tag="ACHOQY_000059"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9676225.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(62133..63119)
                     /gene="zipA"
                     /locus_tag="ACHOQY_000060"
     CDS             complement(62133..63119)
                     /gene="zipA"
                     /locus_tag="ACHOQY_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9676226.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            63349..64110
                     /gene="cysZ"
                     /locus_tag="ACHOQY_000061"
     CDS             63349..64110
                     /gene="cysZ"
                     /locus_tag="ACHOQY_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9676227.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            64295..65266
                     /gene="cysK"
                     /locus_tag="ACHOQY_000062"
     CDS             64295..65266
                     /gene="cysK"
                     /locus_tag="ACHOQY_000062"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9676228.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            65650..65907
                     /gene="ptsH"
                     /locus_tag="ACHOQY_000063"
     CDS             65650..65907
                     /gene="ptsH"
                     /locus_tag="ACHOQY_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9676229.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            65952..67679
                     /gene="ptsI"
                     /locus_tag="ACHOQY_000064"
     CDS             65952..67679
                     /gene="ptsI"
                     /locus_tag="ACHOQY_000064"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9676230.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            67720..68229
                     /gene="crr"
                     /locus_tag="ACHOQY_000065"
     CDS             67720..68229
                     /gene="crr"
                     /locus_tag="ACHOQY_000065"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9676231.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(68271..69122)
                     /gene="pdxK"
                     /locus_tag="ACHOQY_000066"
                     /gene_synonym="thiJ"
     CDS             complement(68271..69122)
                     /gene="pdxK"
                     /locus_tag="ACHOQY_000066"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9676232.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            69227..69595
                     /gene="yfeK"
                     /locus_tag="ACHOQY_000067"
     CDS             69227..69595
                     /gene="yfeK"
                     /locus_tag="ACHOQY_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP9676233.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(69598..70509)
                     /gene="cysM"
                     /locus_tag="ACHOQY_000068"
     CDS             complement(69598..70509)
                     /gene="cysM"
                     /locus_tag="ACHOQY_000068"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9676234.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(70643..71740)
                     /gene="cysA"
                     /locus_tag="ACHOQY_000069"
     CDS             complement(70643..71740)
                     /gene="cysA"
                     /locus_tag="ACHOQY_000069"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9676235.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(71730..72605)
                     /gene="cysW"
                     /locus_tag="ACHOQY_000070"
     CDS             complement(71730..72605)
                     /gene="cysW"
                     /locus_tag="ACHOQY_000070"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9676236.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            complement(72605..73438)
                     /gene="cysT"
                     /locus_tag="ACHOQY_000071"
     CDS             complement(72605..73438)
                     /gene="cysT"
                     /locus_tag="ACHOQY_000071"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9676237.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(73438..74454)
                     /gene="cysP"
                     /locus_tag="ACHOQY_000072"
     CDS             complement(73438..74454)
                     /gene="cysP"
                     /locus_tag="ACHOQY_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9676238.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(74625..75416)
                     /gene="ucpA"
                     /locus_tag="ACHOQY_000073"
     CDS             complement(74625..75416)
                     /gene="ucpA"
                     /locus_tag="ACHOQY_000073"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9676239.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(75545..76402)
                     /gene="murR"
                     /locus_tag="ACHOQY_000074"
     CDS             complement(75545..76402)
                     /gene="murR"
                     /locus_tag="ACHOQY_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP9676240.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            76566..77462
                     /gene="murQ"
                     /locus_tag="ACHOQY_000075"
     CDS             76566..77462
                     /gene="murQ"
                     /locus_tag="ACHOQY_000075"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9676241.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            77466..78890
                     /gene="murP"
                     /locus_tag="ACHOQY_000076"
     CDS             77466..78890
                     /gene="murP"
                     /locus_tag="ACHOQY_000076"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9676242.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            78895..80199
                     /gene="pbp4b"
                     /locus_tag="ACHOQY_000077"
     CDS             78895..80199
                     /gene="pbp4b"
                     /locus_tag="ACHOQY_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP9676243.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(80257..81156)
                     /gene="yfeX"
                     /locus_tag="ACHOQY_000078"
     CDS             complement(80257..81156)
                     /gene="yfeX"
                     /locus_tag="ACHOQY_000078"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9676244.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(81252..81827)
                     /gene="yfeY"
                     /locus_tag="ACHOQY_000079"
     CDS             complement(81252..81827)
                     /gene="yfeY"
                     /locus_tag="ACHOQY_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9676245.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(81888..82337)
                     /gene="yfeZ"
                     /locus_tag="ACHOQY_000080"
     CDS             complement(81888..82337)
                     /gene="yfeZ"
                     /locus_tag="ACHOQY_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP9676246.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(82324..82749)
                     /gene="ypeA"
                     /locus_tag="ACHOQY_000081"
     CDS             complement(82324..82749)
                     /gene="ypeA"
                     /locus_tag="ACHOQY_000081"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9676247.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            82963..83832
                     /gene="amiA"
                     /locus_tag="ACHOQY_000082"
     CDS             82963..83832
                     /gene="amiA"
                     /locus_tag="ACHOQY_000082"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9676248.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            83836..84735
                     /gene="hemF"
                     /locus_tag="ACHOQY_000083"
     CDS             83836..84735
                     /gene="hemF"
                     /locus_tag="ACHOQY_000083"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9676249.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAIGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(84741..85793)
                     /gene="eutR"
                     /locus_tag="ACHOQY_000084"
     CDS             complement(84741..85793)
                     /gene="eutR"
                     /locus_tag="ACHOQY_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9676250.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(85839..86339)
                     /gene="eutK"
                     /locus_tag="ACHOQY_000085"
     CDS             complement(85839..86339)
                     /gene="eutK"
                     /locus_tag="ACHOQY_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9676251.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(86352..87011)
                     /gene="eutL"
                     /locus_tag="ACHOQY_000086"
     CDS             complement(86352..87011)
                     /gene="eutL"
                     /locus_tag="ACHOQY_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9676252.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(87021..87908)
                     /gene="eutC"
                     /locus_tag="ACHOQY_000087"
     CDS             complement(87021..87908)
                     /gene="eutC"
                     /locus_tag="ACHOQY_000087"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9676253.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(87929..89290)
                     /gene="eutB"
                     /locus_tag="ACHOQY_000088"
     CDS             complement(87929..89290)
                     /gene="eutB"
                     /locus_tag="ACHOQY_000088"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9676254.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(89302..90705)
                     /gene="eutA"
                     /locus_tag="ACHOQY_000089"
     CDS             complement(89302..90705)
                     /gene="eutA"
                     /locus_tag="ACHOQY_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9676255.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(90702..91928)
                     /gene="eutH"
                     /locus_tag="ACHOQY_000090"
     CDS             complement(90702..91928)
                     /gene="eutH"
                     /locus_tag="ACHOQY_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9676256.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(92045..93232)
                     /gene="eutG"
                     /locus_tag="ACHOQY_000091"
     CDS             complement(92045..93232)
                     /gene="eutG"
                     /locus_tag="ACHOQY_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9676257.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(93222..94058)
                     /gene="eutJ"
                     /locus_tag="ACHOQY_000092"
     CDS             complement(93222..94058)
                     /gene="eutJ"
                     /locus_tag="ACHOQY_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9676258.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(94069..95471)
                     /gene="eutE"
                     /locus_tag="ACHOQY_000093"
                     /pseudo
     CDS             complement(94069..95471)
                     /gene="eutE"
                     /locus_tag="ACHOQY_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(95483..95770)
                     /gene="eutN"
                     /locus_tag="ACHOQY_000094"
     CDS             complement(95483..95770)
                     /gene="eutN"
                     /locus_tag="ACHOQY_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9676259.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(95877..96170)
                     /gene="eutM"
                     /locus_tag="ACHOQY_000095"
     CDS             complement(95877..96170)
                     /gene="eutM"
                     /locus_tag="ACHOQY_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9676260.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(96209..97225)
                     /gene="pta"
                     /locus_tag="ACHOQY_000096"
     CDS             complement(96209..97225)
                     /gene="pta"
                     /locus_tag="ACHOQY_000096"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9676261.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(97222..98025)
                     /gene="eutT"
                     /locus_tag="ACHOQY_000097"
     CDS             complement(97222..98025)
                     /gene="eutT"
                     /locus_tag="ACHOQY_000097"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9676262.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(98022..98723)
                     /gene="eutQ"
                     /locus_tag="ACHOQY_000098"
     CDS             complement(98022..98723)
                     /gene="eutQ"
                     /locus_tag="ACHOQY_000098"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9676263.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(98698..99177)
                     /gene="eutP"
                     /locus_tag="ACHOQY_000099"
     CDS             complement(98698..99177)
                     /gene="eutP"
                     /locus_tag="ACHOQY_000099"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9676264.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(99190..99525)
                     /gene="eutS"
                     /locus_tag="ACHOQY_000100"
     CDS             complement(99190..99525)
                     /gene="eutS"
                     /locus_tag="ACHOQY_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9676265.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(99818..102097)
                     /gene="maeB"
                     /locus_tag="ACHOQY_000101"
     CDS             complement(99818..102097)
                     /gene="maeB"
                     /locus_tag="ACHOQY_000101"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9676266.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLSEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            102386..103336
                     /gene="tal"
                     /locus_tag="ACHOQY_000102"
     CDS             102386..103336
                     /gene="tal"
                     /locus_tag="ACHOQY_000102"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9676267.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            103356..105359
                     /gene="tkt"
                     /locus_tag="ACHOQY_000103"
     CDS             103356..105359
                     /gene="tkt"
                     /locus_tag="ACHOQY_000103"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9676268.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(105455..106498)
                     /gene="ypfG"
                     /locus_tag="ACHOQY_000104"
     CDS             complement(105455..106498)
                     /gene="ypfG"
                     /locus_tag="ACHOQY_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9676269.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(106624..107199)
                     /gene="nudK"
                     /locus_tag="ACHOQY_000105"
     CDS             complement(106624..107199)
                     /gene="nudK"
                     /locus_tag="ACHOQY_000105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9676270.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(107267..109246)
                     /gene="aegA"
                     /locus_tag="ACHOQY_000106"
     CDS             complement(107267..109246)
                     /gene="aegA"
                     /locus_tag="ACHOQY_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9676271.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            109452..111152
                     /gene="narQ"
                     /locus_tag="ACHOQY_000107"
     CDS             109452..111152
                     /gene="narQ"
                     /locus_tag="ACHOQY_000107"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9676272.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            111316..114429
                     /gene="acrD"
                     /locus_tag="ACHOQY_000108"
     CDS             111316..114429
                     /gene="acrD"
                     /locus_tag="ACHOQY_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9676273.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPQE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(114528..114587)
                     /gene="ypfM"
                     /locus_tag="ACHOQY_000109"
     CDS             complement(114528..114587)
                     /gene="ypfM"
                     /locus_tag="ACHOQY_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9676274.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        28444 a        30404 c        28321 g        27597 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg ggccgtccgt gactcggtgg
      421 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgccccc ccctcatacc cacctctttc
      541 tcttgcgtga cttccctgct gttaatagaa taaaacgatc gataaaacag gtatcgatcg
      601 ttactatcga tcgtttatag tggttaatgg tctgcgaaaa ggcagctgaa tctcttcatc
      661 atgcagaacg gaattgcaca caacggactg atacttctct ggcggatgag agaggggaaa
      721 catttatgtc cgggaaaaac cactttatct gaccatgctg ttttgtacct gccggtatcc
      781 acttttgtgg gtaccggctt ttttattcac cctctgtaag gaaaagctga tgaaacgaca
      841 tctgaacacc agctacaggc tggtatggaa tcacattacg ggcaccctgg tggtggcctc
      901 cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg gttgcgctgt ctcttgctgc
      961 tgtcacatca gtcccggcac tggctgctga cacggttgta caggcgggag aaaccgtgaa
     1021 cggcggaaca ctgacaaatc atgacaacca gattgtcttc ggtacggcca acggaatgac
     1081 catcagtacc ggtctggagt atgggccgga taacgaggcc aataccggcg gacaatggat
     1141 acaaaatggc ggtatcgcca acaacactac tgtcaccggt ggtggtcttc agagagtgaa
     1201 tgccggagga agcgtttcag acacggttat cagtgccgga ggcggacaga gccttcaggg
     1261 gcaggcagtg aacaccactc tgaacggcgg tgagcagtgg gtacatgaag gcgggattgc
     1321 aacggttacc gtcattaatg agaagggctg gcaggccgtc aaatccggcg caatggcaac
     1381 cgacacggtt gtgaataccg gcgcggaagg ggaaccggat gcagaaaatg gtgataccgg
     1441 gcagaacgtc tacggagatg ccgtacgcac caccatcaat aaaaatggtc gtcagattgt
     1501 ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc ggcggcgacc agactgtaca
     1561 tggtcacgca ctggatacca cgctgaatgg ggggtaccag tatgtgcaca acggcggtac
     1621 ggcgtctggc actgttgtaa acagtgacgg ctggcagatt atcaaggaag gtggtctggc
     1681 ggatttcacc accgttaacc agaaaggcaa actgcaggtg aacgccggtg gtaccgccac
     1741 acatgtcacc ctgaaacagg gtggagcact ggtcaccagt actgcggcaa cggttcttgg
     1801 cagcaaccgt ctgggcaatt tcacggtgga aaacggtaag gctgacggcg ttgttctgga
     1861 gtccggcggt cgcctggatg tactggaagg ccattcagcg cagaaaacac gggtggatga
     1921 cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca gatgtcacca tgacatccgg
     1981 tggtgccctg atagcagaca gtggtgccac tgttgagggg accaatgcca gcggtaagtt
     2041 cagtattgat ggcatatccg gtcaggccag cggcctgcta ctggaaaatg gcggcagctt
     2101 tacggttaat gccgggggac aggcaggcaa caccactgtc ggacatcgtg gaacactgac
     2161 gctggctgcc gggggaagtc tgagtggcag aacacagctc agtaaaggcg ccagtatggt
     2221 actgaatggt gatgtggtca gtaccggcga tattgttaac gcaggagaga ttcactttga
     2281 taatcagacg acacaggatg ccgtgctgag ccgtgctgtt gcaaaaggcg actccccggt
     2341 aacgttccat aaactgacca ccaccaacct cactggtcag ggcggcacca tcaatatgcg
     2401 tgttcgcctt gatggcagta atacctcaga ccagctggtg attaatggtg gtcaggcaac
     2461 cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt aacctcgggg tggcaaccac
     2521 cggacagggt atccgggttg tggatgcaca gaatggtgcc accacagaag aaggtgcgtt
     2581 tgccctgagc cgcccgcttc aggccggcgc ctttaactac accctgaacc gtgacagcga
     2641 tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct gaagtccccc tgtatgcctc
     2701 catgctgaca caggcaatgg actatgaccg gattctggct ggctcccgca gccatcagac
     2761 cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt cagggcggtc atctcgggca
     2821 cgataacaac ggcggtattg cccgtggggc cacgccggaa agcagcggca gctatggctt
     2881 cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctc tgacgacagg
     2941 ggtgtatggt gctgcaggcc attcttccgt tgatgttaag gatgatgacg gctcccgcgc
     3001 cggcacggtc cgggatgatg ccggcagtct gggcggatac ctgaatctgg tacacacatc
     3061 ctccggcctg tgggctgaca ttgtggcaca gggaacccgc cacagcatga aagcatcatc
     3121 ggacaataac gacttccgcg cccggggctg gggctggctg ggctcactgg aaaccggtct
     3181 gcccttcagt atcactgaca atctgatgct ggagccacaa ctgcagtaca cctggcaggg
     3241 actctccctg gatgacggcc aggataacgc cggttatgtg aagttcgggc atggcagtgc
     3301 acaacatgtg cgtgccggct tccgtctggg cagccacaac gatatgaact ttggtaaagg
     3361 cacctcatcc cgtgacaccc tgcgcgacag tgccaaacac agtgtgcgtg aattgccggt
     3421 gaactggtgg gtacagcctt ctgttatccg caccttcagc tcccggggag acatgagcat
     3481 gggtacagcc gcagccggca gtaacatgac gttctcaccg tcccggaatg gcacgtcact
     3541 ggacctgcag gccggactgg aagcccgtgt ccgggaaaat atcaccctgg gcgttcaggc
     3601 cggttatgcc cacagtgtca gcggcagcag cgctgaaggt tataacggtc aggccacact
     3661 gaacgtgact ttctgataat caggtcactt cccttcaggc cccggtcatt atgaccgggg
     3721 ccttttactt tcagggatca acagatatgc cgggttgtgc atcacgttta ttgcccgaag
     3781 acccttttac tgccaaatgg catctgaatt gatcacagtc tggccagtta ttacccgatc
     3841 ccgatgggga tattgggtga tctccttcca tgaaaatacg cagctcacca gtggagggct
     3901 tctcagacaa taaacgtcat tcatttcatc tgtcaggccg cgtcttctcc gggagacgcg
     3961 gctttttcat ttataccgct aactcattca taaggagcaa ataatgcgat tagcaagtcg
     4021 ttttggtcat gtaaaccaga tacgccgtga ccgcccgctg acacatgaag aactgatgca
     4081 ctatgtgccc agtatttttg gggaagaccg gcatacctcc cgcagtgaac gctatgcgta
     4141 cattcccacc atcaccgtac tagaaagcct gcagcgggag ggctttcagc ccttcttcgc
     4201 ctaccagatc cgcgtgcgcg acccgggccg ccggggatac accaaacaca tgctgcgtct
     4261 gcgccgtgac ggggagataa acggacaaca tgtccctgaa atcatcctgc tgaactccca
     4321 tgacggtact tccagctacc agatgctgcc gggttacttc aggttcgtct gccagaacgg
     4381 gtgcgtctgt ggccagtctc tgggggaagt gcgtgttcca caccggggaa atgtggtgga
     4441 gaaagttatc gaaggggctt acgaggtggt gggcgtgttt gaccggatag aggagaagcg
     4501 tgatgccatg cagtcgctgg tcctgccgcc accgtcacgc caggcgctgg cacaggcggc
     4561 actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca ttctgacgcc
     4621 acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca tccaggagaa
     4681 tatgctgaaa ggcgggattt ccggtcgcag tgccagagga aaacgtatcc atacccgtac
     4741 cattcacagc atcgacaccg acattaagct caaccgggcg ttgtgggtga tggcagaaac
     4801 actgctggag agcatgcgct gatgcctttt ccctgaaagc gcagtcctga ctcacttccg
     4861 tccctgcctc cggacattct tccattcatt tactttttat aaggaataat ctcatgacaa
     4921 ccgttacgca taattccacc acaccttctg tttccgtaac cgctgcatca gggaataacc
     4981 cacctcagct ggttgctact ctcgtccctg atgaacagcg catcagcttc tggccgcagc
     5041 attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     5101 gtctgtgcga agactactgc ggtggtatct ggaatctgta caccctgaac aacggcgggg
     5161 catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtactg ttcaatgcca
     5221 tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg tattgccgcc tgtctgatga
     5281 cgtacagtca tcatgcctgt cgtatggaga attatgccat gacggtccat tattaccggt
     5341 tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc atcgactgaa
     5401 tggagagaag cacaatgcaa caactttcct ttctgcccgg agagatgacg cccggcgagc
     5461 gcagcctcat tcaacgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     5521 ccttcacctc cacccgtgca gcacgggaat ggctgattct gaacatggcg ggactggagc
     5581 gtgaagaatt ccgggtgctg tatctgaaca accagaatca gctgattgcc ggtgaaaccc
     5641 tcttcaccgg caccatcaac cgcacggaag tccatccccg ggaagtgatt aaacgcgccc
     5701 tgtaccacaa tgccgctgcc gtggtactgg cacacaatca cccgtccggt gaagtcacac
     5761 ccagtaaggc agaccggctt atcaccgaac gtctggtaca ggcactgggc ctggtggata
     5821 tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt tttctccttt gccgaacatg
     5881 gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct tctgtcttca
     5941 gaggtatccc attatgaaaa ttatcacccg tggtgaagcc atgcgtattc accaacaaca
     6001 tccgacatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg gcagtgctga
     6061 agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg
     6121 ccgtaaggac agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgagtggg
     6181 aattctgatg agcagaatta tcgccactac cggaccattc ttagccgatt ttctgtaagg
     6241 attttatcgt gtcagacaca ctccacggga caacacttcc cgacgacaat cacgaccgcc
     6301 cctggtgggg gctgccctgc accgtgacgc cctgtttcgg ggcacgtctg gtgcaggaag
     6361 gtaaccggtt gcattacctt gccgaccgcg ccggtatcag aggccggttc agcgatgcgg
     6421 atgcatacca tctggaccag gcctttccac tgctgatgaa acaactggaa ctcatgctca
     6481 ccagcggtga actgaatccc cgccatcagc ataccatcac gctgtatgca aaagggctga
     6541 cctgtgaagc tgatactctc ggcagttgta gtttcgttta tatggctgtt tatgctaccc
     6601 gattctcaaa tcaattaccg aaagcttctg ttcatcaact aactcattac ttttaatgat
     6661 ggggcccttt ctggggccca agaaattaac aacgctaaca aacaacataa ataacaataa
     6721 attaaataaa aaatttcatt cctgcagggg acaccagata cccttcaaac gaaatctacc
     6781 ttcaccccgt aaaagatagg tttggcagca cacttgcctt atatctactc atttttactg
     6841 caacaggttg aaatctcagc actgtcagaa agcgctgatg actaaacagc cctgggccgg
     6901 gcgatgtaac catcacacag aatcctgata gcgaaatatg gcgtgactcg atacttcact
     6961 ccgcaatgca ttccttgatg aattcgcagg accgtgatac acgggacagg tcgctgaatg
     7021 acgacaatgt cctggaaatc agcgaaccgc gcatctgaag tacatttgag cgactgtacc
     7081 agaacatgaa tgaggcgttt ggattaggtg attattagca gggctaagca ctttagtatt
     7141 attattttcc ggttgaggga tagggagata tcgacaacaa ccggaaaagt ttacgtctat
     7201 attgctgaag gtacaggcgt ttccataact atttgctcgc gttttttact caggaagaaa
     7261 atgccaaata gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag
     7321 cctgcgtggt caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg
     7381 gcaatttgaa aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa atttgccacg
     7441 ctgtatttga agacggatat gacacaaagt ggaacctcaa tagcatgtaa cagcttcact
     7501 aatgaaataa tccaggggtt aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc
     7561 acaacaccga taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc
     7621 gccatgcata gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt
     7681 cctacatcgt gtgattcgaa taaacctgca taaaagacag gaaaaagttg ttgatcaaaa
     7741 atgttataga aagaccacgt ccccacaata aatatgacga aaacccagaa gtttcgatcc
     7801 ttgaaaactg cgataaaatc ctcttttttt acccctcccg catccgccgc tacgcactgg
     7861 tgatccttat ctttaaaaca catgttgatc atcataaata cagcgccaaa tagcgagacc
     7921 aaccagaagt tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata
     7981 gcatagccaa aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc
     8041 attttttcgg tgaagctgtc aagcaaaccg catcccgcca gataccccag gccaaaaaag
     8101 agcgccccca gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa
     8161 atcataaacg gtccggtcaa gaccaggatg aaactcatac accagatgag cggtttcttc
     8221 agaccgagtt tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg
     8281 ttgaccgaat aaagtgtacc taattccgtc cctgtcaacc ctagatgtcc tttcagccaa
     8341 atagcgtata acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat
     8401 gcaaaacgat agtacgcatt tctgaatgga atattcagtg ccataattac ctacctgtcg
     8461 ttaaaaaatt cacgtcctat ttagagataa gagcgacttc gccgtttact tctcactatt
     8521 ccagttcttg tcgacatggc agcgctgtca ttgccccttt cgccgttact gcaagcgctc
     8581 cgcaacgttg agcgagatcg ataattcgtc gcatttctct ctcatctgta gataatcccg
     8641 tagaggacag acctgtgagt aacccggcaa cgaacgcatc tcccgccccc gtgctatcga
     8701 cacaattcac agacattcca gcaaaatggt gaacttgtcc tcgataacag accaccaccc
     8761 cttctgcacc tttagtcacc aacagcatgg cgatctcata ctcttttgcc agggcgcata
     8821 tatcctgatc gttctgtgtt tttccactga taagtcgcca ttcttcttcc gagagcttga
     8881 cgacatccgc cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc
     8941 atagatcttc acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg
     9001 tcatcgcagt aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat
     9061 gtaaccattc gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac
     9121 tggggcggac cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg
     9181 tggatgtccg gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca
     9241 gcgttctttg cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta
     9301 ttccgcctaa tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca
     9361 gtaggcgccc gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt
     9421 tggctgacat ttttttccct taaattcatc tgagttacgc atagtgataa acctctttct
     9481 cgcaaaatcg tcatggattt actaaaacat gcatattcga tcacaaaacg tcatagttaa
     9541 cgttaacatt tgtgatattc atcgcattta tgaaagtaag ggactatatt tttataaaag
     9601 ttaacgttaa caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga
     9661 ttgcatgcgg cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat
     9721 ccccattttc acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg
     9781 tttaacgatc gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca
     9841 atgcactggg gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg
     9901 ctagcgccag gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac
     9961 aatggtgtcc tctcacttat ctacaccgga cacgtctggc tcgatggtgc aggtaatgac
    10021 gatgcaattc gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa
    10081 cagggtgtga tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg
    10141 cgtgaagccg acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag
    10201 atcctgcttt atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac
    10261 gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag
    10321 cattatctga tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc
    10381 tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg
    10441 cattttactg aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag
    10501 gatggtcggc gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa
    10561 cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa
    10621 cttctacaac gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct
    10681 ccccgcacaa tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag
    10741 ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga
    10801 atgcggctgt atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac
    10861 gagaatttag acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg
    10921 atatttatcg atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt
    10981 agtcgaatct atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg
    11041 gctgtgctgc aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac
    11101 ggatcaacag cgggcaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat
    11161 gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta
    11221 aaaacagatg cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga
    11281 ttcgtacccc cttcgccaga agaacctgat aagccacaaa ggctgcgcga tcgttaccac
    11341 atatcagaac atcaaaatct ggtttgcccg atttgaagtg gtcattgagt aaacttgcga
    11401 gatcggtgta gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc
    11461 gtccagcatc acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac
    11521 tttccggtag ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg
    11581 attcatattg tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca
    11641 cacagttcgc caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca
    11701 gccccattgt agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc
    11761 gggcagcgtc atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg
    11821 cggtttgctc aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg
    11881 ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat
    11941 caggaacata attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca
    12001 cagattctgc attatgcatc acccgggaga ctgtcatcat cgacactccc gccaggcgtg
    12061 cgacatcctt taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga
    12121 aacagacgtc actggcgaga tccaacgata ttctcaacgc cctatcaccc gacacggcaa
    12181 tacaataaaa aataacaata attgccggat aattgtactc aattccgcct ctgttctcgc
    12241 caacgagtaa ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag
    12301 aagaaaatat aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt
    12361 attgcgattg ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc
    12421 tggctagtga agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca
    12481 gcgacattta tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc
    12541 taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc
    12601 cgttggtaaa ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct
    12661 cattggctga aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg
    12721 cgcgcttatc ccgttttgca ccctatctgg caaaagcatt tcctgaaact gctgccactg
    12781 gggggattat tgaatcagaa ctggttgcca ttccagctat gcaaaaacgg ctggaaaaag
    12841 aatatcagca accgatcagc gggcaactgt tactgaaaaa agatagccat ttgcccattt
    12901 ccggctccat aaaagcacgc ggcgggattt atgaagtcct ggcacacgca gaaaaactgg
    12961 ctctggaagc ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt
    13021 ttaaacagtt ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat
    13081 caatcggcat tatgagcgcc cgcattggct ttaagatgac agttcatatg tctgctgatg
    13141 cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc
    13201 aagattatgg tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt
    13261 tctttattga tgacgaaaat tcccgcacgt tgttccttgg gtattccgtc gctggccagc
    13321 gtcttaaagc gcaatttgcc cagcaaggtc gtatcgtcga tgctgataac cctctgtttg
    13381 tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcgga cttaaactgg
    13441 cgtttggcga tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt
    13501 taggcgtcca tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc
    13561 ttaccgcagc ggatggcctt gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg
    13621 agcgtctgct ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct
    13681 ggctggcgca ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac
    13741 ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat
    13801 tgcgtaatgc cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga
    13861 tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa cgcagcatcg caatcctttc
    13921 cctgggtgag cgatgctgcc gatggcgcag acttaagatc cccgctctta cccgctataa
    13981 ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa
    14041 ccaaaaccgt aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa
    14101 tagaaagtga ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac
    14161 catagtattt atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg
    14221 ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg
    14281 acgtaccaac tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa
    14341 atttattatc tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa
    14401 cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa
    14461 atgtcacaga acgccagtaa tagcaaaccg catacatcaa aaaactaaat gtcactaaca
    14521 accgcatgtc tattttgttg ccataacgtc ctatcaaagg tgaaattaat agtggcatga
    14581 tgccgatggg cgcataagca agtccggccc atatcgcatt ataccccatc gtttcctgga
    14641 gtaactgcgg cataaggacg atcgctccag agtaaaataa atacgcgcat gtgatactca
    14701 caataccaat ggtgaagtta cgggacttaa acaaactgag atcaagaatc gggttctctg
    14761 aggtcgactc ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta
    14821 ttattgtact cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc
    14881 caccaacacc gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagttt
    14941 cagtttctct tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga
    15001 cattgattaa aaatatccaa ccccagctaa agttatcgca aatataaccg cccaatatcg
    15061 gcccacatat cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt
    15121 tttctggtgg ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca
    15181 ttaatccctg aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca
    15241 ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc
    15301 tttgtgccag cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga
    15361 taacccaggt gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga
    15421 cgttagaaat agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa
    15481 cgcaccataa cgtcccaccg gttaatggtg ccggagtcga tttagtgatt gccattaaag
    15541 ttgtccatta tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac
    15601 taaagcctta ctggtataag ccggcatgga cgtcacggtt gaagccagat caggcatctc
    15661 agcaatgtct tcgttcttcg tatcaatagt tgcagtcatc gataaaccaa tgcgtaaggg
    15721 gtgttccatg agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt
    15781 gatccagttc cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat
    15841 attgatccct gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat
    15901 attgactgat tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac
    15961 ccacatttga cgtgccggta cgacagccat taacgattgt ccgggactca ctgtttcgcc
    16021 gacctgaaca ctccgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt
    16081 aagcgccaac caggcttctt tagttgcatc cgccgcttcc acgacttgtg gctgacggtt
    16141 taatggtgtg ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag
    16201 tgccgctttg ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac
    16261 cccctgcttc gctaacggta ctcgacggtt ataatcttct aaagattgtt gatactgaat
    16321 acgtgctgaa gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg
    16381 ccgaacaata tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt
    16441 atccagtgaa actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac
    16501 agtgacacta cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc
    16561 atctgtacta atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc
    16621 aataaataaa actactgcca ataaagaaaa gtattttctt ctgttagaat gttttttatt
    16681 tgaattaatc tgttccacta ttatctctca tttctcatag atgaaattta tgaattgaat
    16741 gaaaagagca ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa
    16801 cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt
    16861 ttgcaataca attcttacgc ctgtaggatt agtgagaaga cttatagtgc taacttgaaa
    16921 ctataaatca tcggtataat ccctgatttt attgttgaca tttcatttat gccgactatt
    16981 tatatggtat acttgtcgaa ttatcttaaa ggaagctcag attttcttat ttttattgag
    17041 aaaatgagat gacgccttat gtctgtatta ctacagggag aagggagatg cttcattgca
    17101 aagggaataa tctatgaacg caataattat tgatgaccat cctcttgcta tcgcagcaat
    17161 tcgtaattta ttgatcaaaa acgatattga aatcttagcg gagttgactg aagggggaag
    17221 cgccgttcag cgggtggaaa cacttaagcc tgatatcgtc atcattgatg tcgatatccc
    17281 cggagttaac ggtatccagg tgttagaaac gctgaggaag cgccaatata gcggaattat
    17341 tattattgtc tccgctaaaa atgaccattt ttacgggaaa cattgtgctg atgctggcgc
    17401 taatggattc gtgagtaaaa aagaaggcat gaacaatatc attgcggcta ttgaagctgc
    17461 aaaaaatggc tactgctatt tccccttctc tctcaaccgg tttgttggaa gtttaacgtc
    17521 cgaccagcaa aaactcgact ccttatcgaa acaagaaatt agtgtcatgc ggtatattct
    17581 tgatggcaag gataataatg acattgctga aaaaatgttc atcagcaaca aaactgtcag
    17641 cacttataaa agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg atctttacac
    17701 attcgcgcaa cgtaacaaaa tcggctaatc acatgaagtt tttaccctat ttttttcttc
    17761 tctgttgtgg tctttggtcg accataagtt tcgcagacga agattacatc gaatatcgtg
    17821 gcatcagtag taacaaccgt gtcacacttg atccagtacg tctgagcaac aaggaattac
    17881 gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa acggctacct
    17941 tgttgcatac cgattcgcag caacaggttc gtggtattaa tgctgattat ttaaatcttt
    18001 taaaaagagc gttaaatatc aaattaacac tccgggaata cgccgatcat caaaaagcaa
    18061 tggacgcgct tgcagaaggt gaagtcgata tagtgttatc acatttagtt acttcgccgc
    18121 ctcttaatag tgacattgct gcaaccaaac cattgataat tacctttccg gcgctggtaa
    18181 ccacccttca cgactcaatg cgaccgctta cctcaccaaa accagtaaat attgctcggg
    18241 tagcaaatta ccccccagac gaggtaattc atcaatcgtt tccaaaagca acaattatct
    18301 cttttacaaa tttatatcag gcattagcat ccgtatcagc cggacagaat gattacttta
    18361 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
    18421 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatccg
    18481 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
    18541 aagtttcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
    18601 tcactgaaca tgaaaaacag tggattaagc aacatcccga tttaaaggtg ctggaaaatc
    18661 cttactctcc tccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
    18721 atattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
    18781 acaatatcca tgccggaaca cagcttaacc ccggtggatg ggatataata cctggcgcta
    18841 tttatagtga agatcgagaa aataatgttt catttgcaga agctttcata acaacgccat
    18901 acgtttttgt catgcaaaaa gcacctgaca gtgaacaaac attaaaaaaa ggaatgaaag
    18961 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
    19021 aatggataca ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg
    19081 atgctctggt cgcgacacag ttaaattcgc gttacatgat cgatcattac tatcctaatg
    19141 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
    19201 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
    19261 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
    19321 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
    19381 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
    19441 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
    19501 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
    19561 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgactca ccctttaaag
    19621 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
    19681 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
    19741 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
    19801 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
    19861 tagcaaaaag tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta
    19921 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
    19981 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
    20041 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
    20101 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat
    20161 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgaccct caggcgttta
    20221 agcaggtctt atcaaattta ctgagcaatg ctctcaaatt taccaccgag ggggcagtaa
    20281 aaattacgac ctccctggtt cacattgatg acaaccacgc tgttatcaaa atgacgatta
    20341 tggattctgg aagtggatta tcgcaggaag aacaacaaca actctttaaa cgctacagcc
    20401 aaacaagtgc aggtcgccag caaacaggat ctggcttagg cttaatgatc tgcaaagaat
    20461 taattaaaaa tatgcagggc gatttgtcat tagaaagtca tccaggcata ggaacaacat
    20521 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
    20581 aacaccccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
    20641 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
    20701 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
    20761 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
    20821 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
    20881 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
    20941 taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
    21001 ccctgaaaaa taatacggcg aacgatctac aactgatgca ggagattctc atgactttcc
    21061 agcatgaaac gcataaagat ctacccgctg cgtttcaagc actagaagct ggcgataaca
    21121 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
    21181 agttgattaa tattagccat cagttagaaa taacacctgt ttcagatgac agtaagcctg
    21241 aaattcttca gttgctgaac tctgtaaaag aacacattgc agagctggac caggagattg
    21301 ctgttttctg tcagaaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct
    21361 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
    21421 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
    21481 attacccggc atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg
    21541 caatttaatg gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat
    21601 tctggctaac caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag
    21661 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
    21721 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
    21781 aatcggctta tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
    21841 cagacgttgt ggtgacttcc ctgtcgcgat atatgccatc agaccatgct ccagaaaact
    21901 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
    21961 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
    22021 tgtaccaacg cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat
    22081 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
    22141 tgacgatgaa gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc
    22201 cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
    22261 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
    22321 atgattaata aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt
    22381 atcatcacca tgacccggcg gctcaacttt aattaccctt gcgcccatat tacaaagaag
    22441 ttgagttccg aaaggtccat taaggacatg tgtcatatcg ataactaata agccttcaaa
    22501 cggccctttg ctttcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa
    22561 agacacttcg gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga
    22621 cataaatcca caagggagcc gtgacgacaa agcccagtac gctcacagcc aatgacgctg
    22681 taccagtacg ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg
    22741 gtaatgcgcc tgccagtacc atcatctgca gatgttcgct gttcaaatga catgccatac
    22801 caacgagaag cagtgccagt ggcatcagaa tcagcttcag gaaggtgtta taagcaattt
    22861 cggcactgaa ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc
    22921 ctgagttagc cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc
    22981 caaccaacac caggatcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag
    23041 aaattaatgc gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca
    23101 aatacagacc aataggaata gtaattgcgt taacaataat agaaataatt gctaccacta
    23161 aacctgttga tacggaatca ccataaatag gatcgagaac tgcaaacccc aggaatccaa
    23221 tggtaggtga acccgcaatt aatgcacata cagctgcttc tgcatgggta cgtttaaaaa
    23281 atttgtagca accaaaccag gagaaaaaga aacatccgac aataaccaca agtgatacaa
    23341 gggtcagacg agtgtccgca aaaatcattt ccctgtttgc ccgggtaata gaaacaaata
    23401 gagccgcagg aagagcgtag tttaatacca gtttattaaa tgcgcgggct tgatcttctg
    23461 aaaaagtttc tcgtctgccg ctaaaataac ccaacagcat gattacgata ataggcaata
    23521 aatcgccaat aaaaaatgtt agcataatag tatttccgat aagggtcccc tcaccatcgc
    23581 ataatgagtt ggcaagagga gacgatgtca gtatttaatt accagcgact tgttttgggt
    23641 taagtttggt aatatggcca ctttcagttc ctgctgcagg gtcgatgacc acattaataa
    23701 tggtcggttt gcgcgactga ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg
    23761 tgacgttata gccaacgcca cgaaacgcat ccattaattt gtcatacctt gcatggtgca
    23821 acagatccgt tggtgatggt gcaccagcgc cactgagatc aacaccgtct cctctgtaga
    23881 tgccgccatt attaaaaata acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa
    23941 tttccatccc actgaaacca aaagcactat caccttcaat ggcgacaacc ggagaaccag
    24001 aagtcacgct agcaccgatg gcatagccca taccgatgcc catgacaccc caggtaccac
    24061 aatccagacg acgacgtggt ttatacatat caataatatt tcgtgcatta tccagggtat
    24121 ttgcaccttc attaactaaa taaatatcct ggttctcgcg caatacgtcg cgcacagcac
    24181 ttaatgcatt aaagtaattt aatggttgag tatctgtact taatttttca tgcatttttt
    24241 gtgcattttg ctgcttgtgg atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg
    24301 tgttttgttt cagttctgcc agcatacctt gcatactgga tgcaatatca ccaacgactg
    24361 gcacagcaat ggggcggttg ctgtcaattt cctgcggttc aatatccagt tgaataaact
    24421 gtgtatctgc cgcccatcct tttttaccgt gtgccaataa ccaattcagt cgtgcaccaa
    24481 caagcatgac aacgtcagca tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg
    24541 gatgcgtatc ttcaaggatc cctttcgcca tagacattgg caggaatgga atctgagtac
    24601 tttcaataaa ctcacgaagc tgttcatcag cttgtgaata cgccgcgcct ttgccaagga
    24661 taattaatgg ccgttcagct tttgctaaaa gcgaaattgc gctagtgact gacttcgggc
    24721 atggcaataa tgctggcgac ggattttcaa ctttaacaat cgtggttaac gcttcgtctt
    24781 tttccatcgt cgcggccagg acatttgctg gcaaatcaag ataaactccg ccagggcgac
    24841 ccgatacaga gacccggata gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa
    24901 cgcgaaatgc tgctttggca tacggttttg ccgcattcat ttggtccagc tcttcataat
    24961 caccttgctg taggtcgacg atcgcgcggt cgctggagcc gctaatcatg atcatcggaa
    25021 aaccatttac cgttgcgttg gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa
    25081 ctgtcaggca gatccccggt ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg
    25141 ccgactgctc atgacgaaaa ccaatataac gaatgccttc cgcctgggca tggcgtgcca
    25201 tatccgtcac aggaatacct acaacaccat aaatagtgtc aatattattc tgttttaatg
    25261 cttcaacgat gatatgcata ccatctgtca tttgaagttg atctgacatg attaataacc
    25321 tcgaaatttt tataaattgg gagccagaag cccccgttaa tatcagatgg cgtggttttg
    25381 cttcattgca gcaatttcat cgtcgctata acccagctcc tgcaatacag cagcggtatg
    25441 ttcacctaat agcggcgcag ctttaatatc cggcgtaaag gcagagaatt tcattggaca
    25501 accaacggtc agatattttc cacgcaacgg ttgttccact tcaacaacac tgccactttg
    25561 gcgcaaagag ggatcaagtg aaatttcttt catacttaaa accggtgcac aaggaatatc
    25621 aaactgagtc aaataggcca ccgcttcatg tttatcaata gtgacagtgt atttttcgat
    25681 ttcagcaaaa atatcgaaaa tatgtggctg tcgtgcatgg gctgtactgt atgccggatc
    25741 ggtaatccat tctggtttgc cgatggcttt acaggtgttt tcccagtttt gctcctgaat
    25801 agtgaaataa atataggcgt taggatcggt ttcccagcct ttacatttca ggatccagcc
    25861 aggctgaccg ccaccacctg cattaccacc gcggggaact gcatcaccaa atgtaccatt
    25921 cggatactgc gggtattctt ccagataacc caatttatcg agacgctgct ggtcacgtaa
    25981 tttcacgcgg caaaggttca atacggcatc ctgcattgac atggtgactc gttgcccacg
    26041 ccccgttttt tcgcgatgca gcaaagcagc aagtaaaccg atcagcaaat gcattccggt
    26101 gttgctgtca cccaacgctg cagcgcttac cagcggcgga ccatcccaaa aacccgtagt
    26161 ggatgccgcg ccacccgctg cctgagcaac gttttcatag gcttttacat tcacataagg
    26221 cgaacactca tcaaaccctt tgatcgaacc aaaaatcaga cgtggattga tttcttgaat
    26281 atgctcccag gtgaagccca tgtgatcaat ggcccctgga tgaaagttct cgactaagat
    26341 atcagcttcg cggatcagct tttccattac ctctttgcct tccgctgttt tggtatttaa
    26401 ctcaatagaa cgtttgttac tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat
    26461 atctcgcagc tggtgacgcg ttacgtcacc aacgccggga cgttcaattt taataacgtc
    26521 agcgccaaac caggccagca tttgagtaca agatgggcca gattgcacac cggtgaaatc
    26581 gagaacttta attccttgaa gtggagttga catagcaata cctattagtt aattaaatat
    26641 gtgttaattt agcgtttatt taaggcgtaa tatcttctat aaataacccg caaaatatcg
    26701 tcgtagctgc actcgatatt tttaaaatcc tgtatcaagt gctgaaaatc atttcatcgc
    26761 cacttccaat tcctttctgc atggtaagta gtgaggacga aaagaagaat atgataaata
    26821 ttgattcgtg gtaatcagag agagtcgcga catttgtgat gttattctta caactgtaag
    26881 aagcgccggt gatttacaag taagggtatg attacgcttt ttaaagcgta ccataaaaca
    26941 taacataata aatgatttgt gatgacacgg gattattggg atttactttg aggaagataa
    27001 aatgcgtgtt tatagaaccg cgagcgccaa ataattttat gaccacaacc tgtcgtattg
    27061 tggtcataaa aaattgtcgg ctggagggag ttaaatcctt tacctcaata tactgcctgt
    27121 cagcattagt tcacaaacag tgcttcgctg tcgacgatga ttccatcttc cgcaccttta
    27181 agcgccaggt ttgcctcgta gtattgcttt ttatcaagca gtttctgcgc atcagcaagt
    27241 gcattacgag tctgtttaag cggcatcaga tattggtttt ccattacgcc aacaccggcc
    27301 aggcgaagtt cttccattgc gcccttttta tcccctttcg ccattttttc attggcaatt
    27361 tttatcgcgg cttctttttc gggagttgct acataactct cagaaattcc gacagatgca
    27421 ttaatgacaa tatattggtc atcattgaga ttggtctttt tacctggctt agcgaacttt
    27481 gcccattcgg tactgtcatc tgacagcaaa gcggaagctt cattggtcag ttcttttgcc
    27541 ttttctggat cgccatgaaa cagagccagg cgcgctacct gcacgtcacg catcgcatat
    27601 aaaccttgtt cagagatacg ctctgctgct gccgttttct gggtttgctg aacttgctga
    27661 gtttgctgtt gtgccgctac cggtgcatta tcagcagccc atacagttga agatgccaga
    27721 attgctgtga ccatcgtagc cataattaaa cgtttcatac ttacctcatt ctttaacagt
    27781 tttagaaaac gccgccatca tattgggtga gcatgaaatg agaaatcaga gagcaacggt
    27841 cattttgaaa gggttatctt acagttgtag ttattatcgc aagaagtatt ttagtcttac
    27901 aattgtagga atctgcttcc tgtcgcagag aactattctt atgtaccaca aagagatgtc
    27961 atttattata tcgatgtaaa tattgattaa ttatatgtaa atacagccat aacatcgtag
    28021 gttctcagtg aaccattttc aggataactt tctatgaaag taaacttaat acttttcagc
    28081 ttatttttat tggtctctat tatggcatgc aatgtttttg cattttccat ttctggtggt
    28141 gtaagtgaga ggagctataa agagactgaa aaaacatcag cgatgacaac cacacactct
    28201 acaaaacttc agccatcaca ggcgattttg tttaagatga gagaagatgc gccaccatta
    28261 aacctcacag aagaaatcac gcctacttat ccgacaaagg cgaattatct tattcatcct
    28321 gtgcgatagc gtctgtcaca tggcaggtga acaatcatgt ctgatgctga tgtgaacacc
    28381 tgcccaaatg agccatcctg acccactatc gcctgccata ccccaccata aagcgaatca
    28441 tcaagccaca agccggggca agaaacataa atatccccgt cactagttca ttaccgccag
    28501 ttaaccacat ggtgaagtaa taaatgagtg cagcgaggat caccatgccg aagaaagaag
    28561 agaccgccga taccacgttc agtacctgac cgatgtagcc gcttacgcag acaatacaca
    28621 gggcgagaaa catccataaa taaatcatag ctcaacctcc tgccgcgcta aaaaacctgt
    28681 aacttcagtc actttaatag taagacaatc cttaacctct ggcaattttt gctaagcatc
    28741 aatgaaaaca gcatgttaaa tgcaagactg ttgtgtacgg aaaaatattt actttgcacg
    28801 attaataatc agtagctgaa agcagtcagc gagtgataaa gtacagatac cgcattcatg
    28861 ctcctcgtgg ttatgtcctg actagtcttt acactcttta caggaaccat tgtcgtacat
    28921 gatggcccaa ccaattgagt gtaccgctgt cgacatggaa tcttctgcga tacaacaatt
    28981 cgtatctaca gaaggtaact atgtttccac aatgcaaatt ttcccgcgag tttctacatc
    29041 ctcgctactg gctcacatgg tttgggcttg gtgtactctg gctttgggta cagcttcctt
    29101 atcctgttct ctgctttctc ggcacgcgta ttggcgcaat ggcgcgacca ttcctgaaac
    29161 gtcgtgaatc tatcgcccgt aaaaacctgg aactttgttt cccgcagcat tctgcggaag
    29221 aacgcgagaa gatgattgcc gaaaactttc gttcactcgg catggcgctg gtagaaaccg
    29281 gcatggcatg gttctggccc gacagtcgcg tacgtaaatg gtttgatgtt gaagggttgg
    29341 ataaccttaa acgcgcacaa atgcaaaatc gcggcgtaat ggttgtcggc gtccatttta
    29401 tgtcgctgga actgggcggc cgcgtgatgg gactgtgcca accaatgatg gctacctatc
    29461 gtccacataa taatcagctg atggaatggg tgcagacccg tgggcgcatg cgctctaaca
    29521 aagcgatgat cggcagaaat aatctgcgcg gcattgtcgg tgcactgaag aaaggtgaag
    29581 cggtatggtt tgctcccgat caggattatg gtcgtaaagg cagctccttc gcgccgttct
    29641 ttgcggtgga aaatgtcgcc acaaccaatg gcacctatgt tctctcccgt ctctctggcg
    29701 cagccatgtt gaccgtaacg atggtaagaa aagcggatta cagcggatat cgtttgttca
    29761 tcaccccaga gatggaaggc tacccgacag atgaaaatca agccgctgcc tatatgaaca
    29821 agattatcga gaaagagatc atgcgcgcac cggagcagta cctctggatc caccgtcgct
    29881 ttaaaacgcg cccggtggga gaatcgtcgt tgtacattta acaaactgct cttactcttt
    29941 ccctcctcaa atcggccata ataacctcat ggtttttatg gcttttttct taaggttttt
    30001 tccttcgctg aatatctaat tattaccgtc agaaattaaa ctgtcgccga tatgagacac
    30061 ttgtaagtta ctaaaagtaa ataagaaaat gtctcttgtc tcgtttcagt tttaggcgta
    30121 cattagcgcc gtctcgagca gtttagctca gaattctggg gtgatgccca cggtaaaata
    30181 cagggctttt aaggtcgctt tgcccggtgt caactcactg tatcaggtgt aatgaagtca
    30241 ttcaggcgta acagtaatta cgcggagaga tgtaaagtga aatatttctt tatgggcatt
    30301 tcttttatgg tcatcgtttg ggccggtact tttgccctga tgatctaaaa gcaaactgtg
    30361 caatgaaaaa caggagccat caggctcctg tttgcatttc tgacgacggt gtttattccg
    30421 cgttttcgtg aatatgtttg ctgctggcgg gtagtaaacc gtcggcacgg aacatcgctt
    30481 taatcccacg aatcgcctga cgaatacggt cgcggttttc aatcagtgca aagcgaacat
    30541 gcgtatcacc atagtcgcca aagccaatcc ccggcgagac acaaaccttc gcttcgttaa
    30601 ggagcttctt ggcaaattcc agcgatccca tggccgcata tggttccggg attttcgccc
    30661 agacatacat cgaagccttc ggcatttcaa ccatccagcc cgcttcatgc agccctttaa
    30721 ccaatacatc acggcggcgt ttgtactgtt cggcaatgtc gcgcacgcac tgttgatcgc
    30781 cctccagcgc cgcaatcgcc gccacctgca acggcgtaaa ggtgccgtaa tcgtgatagc
    30841 ttttaatacg agccagggcg ctgaccagcg ttttgttgcc aaccataaag ccgatacgcc
    30901 agcccgccat gttgtagctt ttcgacagcg taaagaactc gactgccaca tcgcgtgcac
    30961 ccggtacctg catgattgac ggcgctttcc agccatcgta gacgatatcg gcataggcca
    31021 ggtcgtggac caccagcaca tcgtagcgtt tcgccagcgc cacaaccttt tcgaagaact
    31081 ccagctccac gcattgcgcg gttggattcg acgggaagcc gaggatcatc atcttcggtt
    31141 tcggataact ttcacgaatg gcgcgctcca gttcgttgaa gaaatcgaca ccttccacca
    31201 gcggcactga gcgtacctgc gccccggcaa tcaccgcgcc ataaatatgg atcgggtaac
    31261 ttggattcgg caccagcacc gtgtcaccat gatccagcgt cgccagcatc agatgcgcca
    31321 ggccctcttt cgaaccaata gtgacgatgg cttctgattc cgggtcgatt tcaacgtcgt
    31381 agcgatcctg ataccagcgg gaaatggcgc gacgtaaccg cggaatgccg cgtgaagtgg
    31441 agtaaccatg cgtgtccggg cgctgggcca cagtacataa tttttcgacg atatgcggcg
    31501 gagtcgcacc gtccgggtta cccatgctga aatcgataat atcttcgccg cgccgacgcg
    31561 cagccatttt cagttcagcg gtgatgttaa aaacgtaggg cgggagacga tcaatgcgcg
    31621 taaagcgacg ttcagggcga gtgtcagcca taatttcctc agattaacgt tagcgcccgg
    31681 accgtccgag cgacgctgcc acaatggtgg ctgttttgaa aatagcctga ttaatttctg
    31741 tctgtccagc gttttagaac atttttatca atggctttaa atgaggaact ccacttcaaa
    31801 aacaggaaat ttttctacgc taacaagaaa attaccacca attttattaa cattataatt
    31861 tcaataaatt taccaaccag aagtgcatta tcaagagata cattgctaat acaccattta
    31921 tctttcaatc cccttttaaa tttatggttt ttcctcaaaa acggcctgct ggtcatcagc
    31981 cttcaggtta cctatcatag aggtttaatc cttattcaga gtcacccgtg cacgaaatat
    32041 tcaacatgct gctggcggtc ttcgatcggg cagcgttaat gcttatctgc ctgttctttc
    32101 tcatccgtat ccgcctgttt cgcgaactgt tgcacaagtc ggcgcactcc ccaaaagaat
    32161 tgctcgccgt taccgccatt ttctcgctgt tcgccctgtt cagcacctgg tccggcgttc
    32221 ccgtagaagg ctcgctggtg aacgtacgta ttatcgcggt gatgtccggc gggattctgt
    32281 ttggcccgtg ggtaggcatc attaccggcg tgattgcggg tattcaccgg tatttaattg
    32341 atatcggcgg cgtgacggcg atcccctgct ttatcaccag cattctggcg ggttgtatat
    32401 cgggctggat caacctgaaa atccccaaag cacagcgctg gcgcgtcggt attctcggcg
    32461 gcatgttgtg tgagacgttg accatgattc tggtaattgt ctgggcacca actaccgcgt
    32521 tggggatcga tatcgtctct aaaatcggca ttccaatgat cctcggtagc gtctgtatcg
    32581 gctttattgt gcttctggtg caaagcgttg agggcgaaaa agaggccagc gccgcgcggc
    32641 aggccaagct ggcgctggat atcgccaaca aaacgctacc gctgtttcgc catgtcaata
    32701 gcgagtcatt acgcaaggtc tgcgaaatta tccgcgatga cattcacgcc gatgcggtgg
    32761 cgattactaa taccgatcat gtgctggcct atgttggcgt gggtgaacat aactatcaga
    32821 atggcgatga cttcattagc ccgactaccc gtcaggcgat gaattacgga aaaatcatca
    32881 ttaaaaacaa tgatgaagcc caccgcacac cagagattca ttccatgctg gtgatcccat
    32941 tgtgggagaa aggggtcgtg accggaacgc tgaaaattta ctactgccac gcgcatcaga
    33001 tcacctcgtc attacaggaa atggcggtcg gtctgtcgca aatcatctcc acgcaactgg
    33061 aggtttcacg cgccgagcag ctacgtgaaa tggcaaataa ggcagagctt cgcgccctgc
    33121 aaagcaaaat taatccccat tttctgttta acgctctgaa cgctatttca tcgtcaatcc
    33181 gtctgaatcc ggataccgct cgccagttga tctttaatct gtcgcgttat ctgcgctata
    33241 acattgaatt aaaagacgat gagcaaatcg atatcaaaaa agagctgtat caaattaaag
    33301 actatattgc cattgagcag gcccgctttg gtgacaagct gacggttatc tatgatattg
    33361 atgaagaggt gaattgctgc attcccagcc tgctgatcca gccgttggtg gagaacgcca
    33421 ttgtccacgg tattcagcct tgcaaaggta aaggcgttgt caccatcagc gttgcagagt
    33481 gcggaaatcg ggtacgcatt gcggtgcgag ataccgggca cggcatcgat ccaaaggtga
    33541 ttgagcgggt cgaagcgaat gaaatgccgg gcaataaaat tggcctgctg aatgtccatc
    33601 atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg ccgcctggag ccggggacgg
    33661 aaattgcgtt ttacattcct aaccaacgca ccccagtcgc ctcacaggct acgttattgc
    33721 tttgagccgg agtgatattg tgaaagtcat cattgttgaa gacgaattcc tggcacaaca
    33781 ggaactgagc tggctaatta aagagcacag ccagatggag attgtcggca cctttgacga
    33841 cggtctggac gtgttgaagt ttttgcagca taaccgcgtc gacgccattt ttctggatat
    33901 caatattccg tcgctggatg gcgtgttgct ggcgcaaaac atcagccagt tcgccaataa
    33961 accgtttatt gtgttcatca ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga
    34021 ggcgtttgac tacattctca aaccgtatca ggagtcacgt attaccggga tgctgcaaaa
    34081 actggaagcg gcctggcaac aacagcagac cagcagtacg cctgccgcga cggtaacgcg
    34141 tgagaatgac accattaatc tggtgaaaga tgagcgaata atcgtcacgc caattaacga
    34201 tatctattac gccgaagcgc acgagaaaat gacctttgtc tatacgcggc gtgaatccta
    34261 cgtaatgccg atgaacatta ccgaattttg cagcaaactg ccgccgtcgc attttttccg
    34321 ctgccatcgc tcattttgtg tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa
    34381 taacacctac attctgcgac tgaaagatct ggattttgaa gtgccggtca gtcgcagcaa
    34441 agtgaaagaa tttcgccagt taatgcatct ttaatcggga actttcatga aagcaccagg
    34501 cttgcccgcc gatcaacaat ttttcgccga tctgttcagc ggcctggtgc ttaacccgca
    34561 actactgggg cgcgtctggt ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg
    34621 tattgatttt ccccgtctgg atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc
    34681 aaagcagcag cgaatggtgg aaggagaaat gctgtttatt ccggcacgcg cggctaattt
    34741 accgatcaac aacaaaccgg tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg
    34801 gttatcgttt tacgatagcc gcaccacgtc gttgttgcat cctgctcgcc agatccagct
    34861 tcccagcctg caacgcggtg aaggtgaagc gatgcttacc gccctcaccc atcttagccg
    34921 ttcgccgctg gagcagaata tcattcagcc actggtgtta agtttgctgc atctttgccg
    34981 caacgtggtg aatatgccgc cgggtaattc gcaaccgcgc ggcgattttc tctatcacag
    35041 catttgtaac tgggttcagg ataattatgc ccagccgctc acccgcgaga gcgtggcgca
    35101 gttttttaat atcacgccca atcatctgtc aaaactgttt gctcagcatg gaacgatgcg
    35161 ttttatcgag tatgtgcgtt gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata
    35221 tcatctgtca attcatgaag tggcacagcg ttgcggtttt ccggatagcg actatttttg
    35281 tcgcgttttc cggcgtcagt ttggtctgac gccgggagag tacagcgccc gttttcaggg
    35341 ctaacgtcag aaggttaatt ctgtttccag cagcgtcagg atactttgtg catcttgcgc
    35401 ggcaaacaac gactggcgga agtttttatt caccagttta cgcgccagct gcgagaagac
    35461 tttcacatga ttcatccctt cgttagcacc cagcgtcagc atgatcacca gttcgacttc
    35521 gcccatttct gactgccagt caaccggttt cgccagccgg gcaatgctga tactggaatg
    35581 acggatccac tgagatttgg tgtgcgggat cgctacgcca aaaccaacgc cggtggtgac
    35641 aatctcttcc cgctgccaga catcttcttc cagctcaaac ggatgttcag tgcgcccgtt
    35701 aacgccgagg ttgccgcaca ggaactggat cgcctgctct ttattgctaa aatcctgatc
    35761 aacaaagata ttctccagcg ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt
    35821 gagtaacgct tcaatttcct gcgcactgcg gcattcacat gcctgacgcg ccagttcccg
    35881 acacgcctcg ctatccagtt gacgaagctg gcttttcacc gccggaatac gcgggctact
    35941 catactcagc tcatccaggc ccagcccaag cagtagcggc agataacggc tttcaccgcc
    36001 cagttcaccg caaatgccta cccatttgcc ccgctgatgc gcagtggtaa caatctgctg
    36061 caacatgcgc aggaacgatg gcgtaatcgg gttatacagc ggcgatacgc gcgggttatt
    36121 acgatcgacc gcatacagat actgggtcat atcgttggag ccgatactga agaaatcgac
    36181 ctcatcgcag aagtgatcga tgatgtagca caccgacggc acttccacca tgatccccag
    36241 cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc tcaacgatcg ctttttggat
    36301 ctcgcccttc acccataaga tctgatcgag actgtggacc atcgggatca tcaactgggc
    36361 gttgccaaaa ctggcggcgc gcaaaatcgc ccgtagttga gtgcggaaca ggccagcaaa
    36421 ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg ttctcttcct ggggaatatt
    36481 cagataagga atgcttttat cgccaccgat gtccattgtg cggaagataa tcggcttgtc
    36541 gcccgctgcc agtagcacct gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct
    36601 gtcgcgatcc atatagagca tttcagtacg gaacagtcca acgccttccg cgccgttggc
    36661 aaacgcgcct ggcgcttcca gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt
    36721 atcacgggaa taggccagct gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc
    36781 cagcgtctgt gcgacctgat aataaccgct caccgcgtca ttcgggttaa tcgccagcac
    36841 gccgcactgg gcgtcaagca ccgccggttg cccggcataa cgggcaatcg catccagcgg
    36901 caggccgctc agtaccggga tcgccgaggc gcgggtcaga atcagggtat gcgaggtgcg
    36961 cccagttttc tccagaatca tgcctgcaag attttttaaa tcgaggctca aaaactggct
    37021 tggggttaaa tcctcggcta ccagaatagt cggtttttcc agcaccagat tgttgcgcgg
    37081 cttcagttcc ggccaggtga tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac
    37141 acgttcgcgc agataatcac tggcagaagc agaaagtttg gcgcaaacct gctccatatt
    37201 gctgatgatc gccgccccca gcccttgatg ctgttctgtc atcagacgac ggatattgcc
    37261 cgcgaattca tcatcctgaa tcagcgacaa atgggcgctg aggatagttt tgctttcgcc
    37321 gtcacgctca cgcaattgct ggttcagttg ctcggcaagc gttgccaggc tgtgctccag
    37381 ccgggtggaa tcttgcgcac tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg
    37441 taacagggtc agcgtaccca cgccgacgcc gcttgccagc acattgccgt acagtaaatc
    37501 cgggttaagg cggcttaatg aacgcggcag cggatgcgcc gtcagttctg cctgcgtcgg
    37561 ctgaacgctg tcgctgtcga taaagcgcac caggatgtac tcttccagca cgcgccgcgc
    37621 ctgctcttca tcgctgccgc taatgttcag gctgcaactg tcattaaata aggtgccagt
    37681 tccaatcaac gccagcgagc ttttagcatc ggcctttgcg ttctggcgat ggttgaaaaa
    37741 ggtgatttcg ctttgccact ggctgcactg ttctttaagt tcccacgccg gacgagcatg
    37801 tagaccgtta ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc
    37861 aggagatcat ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag
    37921 ggcagataaa agttgctcca tctgcaaaat gtcgcggcaa tcggcaatcg atgccgcgca
    37981 atgtccgtgg cgggtggcag gccccattac cactgtgggt acgccagtac cggttaagtg
    38041 caccgctccg ccgtccgtgc cgccgttgct gaacatatct gcctgcaacg gtacgccaat
    38101 ttcagccgcc acggtttcga tccaggcggt aagttttggc ggcgcaatca gtgacttgtc
    38161 gcttaacacc agcatcgggc cgttaccaat ctggcgatgg ttagccgcgc cataatcaaa
    38221 gtttttcgcc cagcaggcgg tatccagcac aatggcgacg tccggcgaca ccgcgcgggt
    38281 agcggtttgc ccaccgcgta atcccacctc ttcgctggaa cttgccacca gccacacctc
    38341 cgcaggcagt tcggcatcgt gcagctcacg tagtaacgtc accagcagat agcaaccgag
    38401 gcgatcatca aaggctttcc ccatcacccg ctggtgaggg agaacctgaa aagttgtatc
    38461 aaaggtgacg cgatcgcctg gacgaattcc cgcctgcatc acttcgtcat acgagcgcgc
    38521 accaatgtcc acgcgcatgg cgctgacgtc attcccctgc cggtcgccgt caagcaggcc
    38581 tggaattttg cactcttcac gggtggtgat gcgcaccggc tgcagctggc gggcagccat
    38641 gcgcacgttg ccaaccggca gcacatcaat tgccccttcg cgggagatgc tacgcaccat
    38701 aaatcccact tcgtccatat gggcgcagat catcaccttc ggacctgtcg attcattgag
    38761 gcggatcagc accgatccca gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc
    38821 ttccagcagg atctgccgca cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa
    38881 cgcttttaat agcgataaat ccattatgcc tctcccgtga gcaacactgt tttcggcatg
    38941 gcgtagagca cttctgcgcc ttgcggggta accagcacaa catcttcgat gcgcacgccc
    39001 ccttgccctg gcaaataaat ccccggctcc acggtcagta acatgcctgg ctgtagcgtc
    39061 gtggtgtccc gcggtgaaaa acgcggatct tcatgaactt caatgccgat agcatgaccg
    39121 gtgttatgac cgaaatagtg gctaaaacct gcctcggtaa tcacttggcg cgcggcatcg
    39181 tcaacctgct ggcagcgcac gccggggcga attgcagaga ttgctgcgag ctgtgcctgc
    39241 aggacaatct ggtagacgtt aaacagcggg tgagattcgg cgctcacccc ttcgccattc
    39301 accagcaagg tgcgcgtcat atcagagcag tagccctgat acagcgcacc aaaatcgaga
    39361 gtgacaaact cgcccgctgc aacaatcttg tcgctggctt tgccgtgcgg cagcgccccg
    39421 cgccagccgc tggcgacaat ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata
    39481 aaccactcca gttcagcggc tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga
    39541 atatgctccg ctccgcgatc ggcaatccca caggcgaggc ggattatctc cacctcctct
    39601 ggcgttttga tttgccgcag cacatccggc gtggcgctaa ccagtttcgc attgagttca
    39661 gacttccagc ggtgcgcggt ttcccaactc acctgctggc cttcaaaacc gagcgtttgc
    39721 aactgctcat cggcaatgat ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc
    39781 aaatgcagct ggtagccttg cgcacgggct tccacctcaa cgaaatagcg cgaatctacc
    39841 agaatgtgcg cactttcacg gctaataacc acatagcctg atccggtgga gatccccaga
    39901 tgcggctgtt tgttctgccg tgaggagaga agcactgcat ccagttgttg cgccttaagc
    39961 cagtcgcgca gcgaagcgag taatgtcatc tggattttcc ttcttacagg ctatcgatta
    40021 acaatttgcc tttacggaac atcatcagac gcaggaacac caccatcagc gcagttatga
    40081 ctgcccccag cgcgatcccc gccatataga cgcccaggtt agtgaccagc ggccatgccc
    40141 agatagcgga ttccgggaac cattgcactg cgcccagcca gacagcggcg gttgagccga
    40201 caatcgcgcc gaccatatac gacggaatgg cggtgatggg gctttccagc gcaaacggaa
    40261 tcgcaccctc actaatcccc ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa
    40321 gttgagcgtt gaacaggcct ttgccggtta aacgacggtc aataatggtc gccagaccca
    40381 gaccaatcgg aggaataacg atagcgatag aacgagcggt gaccggtaat acgtggtcag
    40441 tggtaaagct gaaggcaacg aaacctgcgg ctttgttgat cgggccacca aggtcgattg
    40501 ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc cttctcacct gcggcagtca
    40561 gcacagtacg gataccgccg ttgatccagc cgccaaacgg ggtgatgacg tagtacatcg
    40621 ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca
    40681 ggaaatccgg taactggatt ttctggttca tccatttcac aaggtaaccg gcaacgatag
    40741 aaataatcag cgcgccaatg aaggtggacg gcaccggcga agaggtcgcc cattgcatag
    40801 tgctgggatc gaagttcagc agttgcgtcg gctgggtaga catcaagccg ccgataaaac
    40861 cagccgggaa cgccagtttg ccaccgatag agttcgccac aaaagcagca aacatcggaa
    40921 tggcaaaacc aaacaacacg ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg
    40981 agaggtcgaa accggagaat ttcccgctat taagagcatc catgatgccg atctcagcag
    41041 gaattttaag ccagctataa gcaatcagct ggctgaaagc gaggatcaca ccgcccataa
    41101 ttaaggtcgg caccatgcgt gaaatccctg acatcacatg ctgcgggagt tcaccccaga
    41161 agctgctttt agaggcctgc ggattcttaa ctgccgctgc cgcaccggat gcgccaggca
    41221 caacggttgc actgcgtttt ttaatggcca taacaattat ccctgtcgaa tattactgtt
    41281 gttcagaggc aatcatctct tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt
    41341 cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg ctcattatct tccggggtaa
    41401 ctgccacgga gtggatgatg atggttgctt cggcgatatc ctgcgccgtc aggcgattct
    41461 ggataccgtc cgcgccctgg gtttcaattt tcacttcata accggcttct accgccgctt
    41521 cttccagcgc ctgagcggcc ataaaagtgt gagccaggcc cattgggcag gcacataagg
    41581 caattagttt cttactcatc gttaattcct cattcagtga tagttgacga gtgaaattat
    41641 gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc atttactgga gaattaatcc
    41701 cgctgtacgg aagtgtgacg caggtcggtc ttgtgagggg acacaaagaa aaattgaatt
    41761 aactgtatga tttaaaagat tatcgggaga gttacctccc gatataacag gaaggattta
    41821 cagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc
    41881 atggacgatg agatacaccg gaatatcata gacatattct ttaaagcgcc ctttatcttc
    41941 aaatgcggca cggaaaccag aggctttgaa gaactcaagg aagcgcggca cgataccgcc
    42001 cgcaataaac acgccgccaa atgtcccgag attgagcgcc agattgccgc caaaacggcc
    42061 cataatgacg caaaacagcg acaacgcgcg gcggcaatcg gtgcagctgt cagccagcgc
    42121 acgttcggta atatcttttg gcttgagatt ttctggcagg cggttgtcag ctttcacaat
    42181 tgcgcgatac aaattcacca gcccagggcc agaaagcacg cgttccgccg aaacatggcc
    42241 aatttccgca cgcagtattt cgaggataat ggcctcttct tcactattcg gcgcaaaatc
    42301 aacgtgaccg ccttcgcccg gcaagcttac ccaacgctta tcgacatgga ccagatgcgc
    42361 aaccccaagc cctgttccgg caccgtaaac cgcaataggt ttaccttcga ccggttctgc
    42421 gccaccaaac tgaatcagat gctctttttt cagcatcggg atcgccatcg atacagcggt
    42481 aaaatcgtta ataatttcca gatggctaaa accgagattc tttttcattt cggcaattga
    42541 gaacgcccag gtatggttgg tcattgccac ccagtcaccg gtaattgggc aagcgatggc
    42601 aatacagccg tctttcacct cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc
    42661 gaggctgggg taatcaagcc ctgaataggt cttagcctgc gagatttcac cactggcaat
    42721 atcacacaga gcaagacgtg cgttggtgcc gcccacatca ccgactaatg catactttgt
    42781 cattcttcaa ctgctccgct aaagtcaaaa taattctttc tcacactgta aatacctggg
    42841 ggcataacaa caacgccgga aaggcaggct ccctgtaaat atcgatctgg gtcacacatt
    42901 tactttatcg tttcagcacc aattgcagcg atgccttttt gcaagctggg caaactaagt
    42961 atctgacccc gcataaggaa tagaacatgc tccatccgcg agccagaacc atgttgttat
    43021 tatcgctccc cgccgtggca attgggattg cgtccagtct tattctgatt gtggtgatga
    43081 aaatcgcctc ggtattacag aatttgctct ggcaacgact gccgggaact ctggggatag
    43141 cccaggattc acctctctgg atcatcggtg tattaacgct aacgggtatt gcggtggggt
    43201 tggttatccg tttcagccag ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg
    43261 gcgcaccagt tccgccctct gcgctaccag gacttatcgt agcattaatt ctcggtcttg
    43321 ctggcggcgt cagcctgggg ccggaacatc cgatcatgac cgtcaatatc gcccttgcgg
    43381 ttgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat ggagtggact attttagcct
    43441 ctgccggaac catcggcgcg ctgtttggta cacctgttgc ggcggcgttg atattttcgc
    43501 aaaccttaaa tggcagtagt gaagttccgc tatgggatcg tctttttgcg ccattaatgg
    43561 cggcagcagc tggtgcactt actaccggat tatttttcca tccccatttt tcactgccca
    43621 ttgcgcatta cggacagatg gaaatgaccg atattctcag cggtgcaatt gtcgcggcga
    43681 ttgccatcgc agcaggaatg gtcgccgtat ggtgcttacc acggttgcac gcgatgatgc
    43741 atcaaatgaa aaatccggtg ctcgtgctag gtattggcgg atttattctc ggtattctgg
    43801 gagttattgg tgggccggtt tcgctgttta aagggctgga tgagatgcag cagatggtgg
    43861 caaatcaggc tttcagcacc agcgattact ttttgctggc ggtaattaaa cttgccgccc
    43921 tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg
    43981 gcgtggcatt agggttgatg ctgcatgagc acgttcccgc cgttccagcg gcaataaccg
    44041 tttcctgcgc tattctcggc atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt
    44101 ttatggcggc agtcgttgta cccaatacca cattgctacc gctgctctgt atcgtcatgc
    44161 ttccggcatg gctgttatta gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac
    44221 agccacccca cgataatgtt tagcaaaaaa tgctcttctt tatgatttaa gagttatggc
    44281 gatgatacgg ggctttacct ccccgtaata ttgcgttaac aggccgctga cggcttcgat
    44341 cgtgaaggag aataacaatg ttcaggtcac tgtttctggc ggccgccctg atggcattta
    44401 ccccgcttgc agcaaatgca ggtgaaatca ccctactgcc atcgatcaaa ttacaaattg
    44461 gcgatcgcga tcattacggt aattactggg acggtggtca ctggcgagat cgtgattact
    44521 ggcatcgtaa ttatgaatgg cgcaaaaacc gctggtggcg tcatgataat ggctatcatc
    44581 gtggctggga taagcgtaaa gcatatgagc gtggctatcg tgaaggctgg cgcgatcgtg
    44641 acgatcatcg cggaaaaggc cgcggacatg ggcaccgcca ttaaacgcgt cctccaatgg
    44701 agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc ctacaaaccg gctctttaac
    44761 gcgtagggcg gataaggcgt ttacgccgca tccgccaata gtgccagatg cgacgctcat
    44821 tcaactacaa tcccagcgcc gtccccacca acaaccagat atttagcgcc acgaccagca
    44881 ctacaatcac ccagcctgtc tgttttacgc gtttgctgtt caccagatcg cccatcaact
    44941 tgctgtcact ggtgaaaatc agcagtggaa ccagcgccag ggcgatacca aaacttaaca
    45001 gcacctgact cataaccaga atccgtgtcg gatctaatcc catcagaatg acaataaatg
    45061 acggcagcat ggtgactgta cgacgcaccc acagcgggat atggaagcga atgaacccct
    45121 gcatcaccac ctgccctgcc agtgtcccca ccaccgttga ggacagtccg gcagcaacca
    45181 ggcttaatcc aaagaccgtt gccgcagcat ggcttaacaa cggttgcagc gtcagataag
    45241 cctcatcaag atcggcaaca ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag
    45301 ccatcatcgc cagattgaca aaaccggcaa tcgtcatggc gatagccaca tcccatttgg
    45361 tggcggaata acgttgttga cgcgaaccgc catgtaaatg ctgagtgagc gaagagtgca
    45421 aataaatcac atgcggcata atcgtcgccc ctaacacgcc tgctgccagg aagaccgcct
    45481 ccgaagtagg taaactcggg atcaccattc ctttacccag ctgcgccagg ttaggctggg
    45541 agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa cagtaacccg ccaatcactt
    45601 tctccagcgg tttttgcccg cgacgttgca gcattaaaat caggaaagtc gcgatccccg
    45661 tcagcaccgc gccctgcaac aacgaaacac caagaatgag tttaaaaccg atcgccgcac
    45721 caataaattc cgccaggtcg gttgccatcg caataatttc tgcctgaacc caatagaacc
    45781 acactacggg acgcggatag tgatcacgaa tctgctccgc cagattttta ccggtggcaa
    45841 tccctagttt ggcagagagg atctgaatca gcatcgccat caggttggcc caaacgacaa
    45901 cccacagtag ctgatagccg aagctagcac ccgcctgaat attggtcgca aagttaccgg
    45961 gatcgatata accaatcgcc gcaatgaacg caggtcccat taatgcgagc ctcatcttgc
    46021 gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca
    46081 tagcctttgc tatgtttcat gctatgccaa acgagaatga ttatcaaatt catttgaatg
    46141 ggttgtggtg atttctttga tagaccagga ttatgactac gaaaagattg atgacttgaa
    46201 tgttatggtt atgtttaatg ttagcacatt tacataactt tcagcttcca tacacaacat
    46261 agcagaaatg tatgacagat cactattttt gaagcttgtc acaggacgtc attatagtgt
    46321 gtgtcagatc tcgttttcct taaccatgtt acatagaatg tgcacggaaa tttaacctgc
    46381 ctcatatttg gagcaaatat ggaccgcgtc cttcattttg tactggcact tgccgttgtt
    46441 gcgattctcg cactgctggt aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt
    46501 caactgcttg ttatcgaagt gttactggcg tggttcttcc tgaactccga cgttggttta
    46561 ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg
    46621 actaacttcg tctttggtag catgaatgat caaggcctgg cattcttctt cctgaaagtg
    46681 ctgtgcccaa tcgtctttat ctctgcactg atcggtattc tccagcacat tcgcgtgttg
    46741 ccggtgatca tccgcgcaat tggtttcctg ctctccaaag tcaacggcat gggcaaactg
    46801 gaatccttta acgccgtcag ctccctgatt ctgggtcagt ctgaaaactt tattgcctat
    46861 aaagatatcc tcggcaaaat ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg
    46921 tccaccgtgt cgatgtccat cgttggtgca tacatgacca tgctggaacc gaaatacgtc
    46981 gttgctgcgc tggtactgaa catgttcagc acctttatcg tgctgtcgct gatcaatcct
    47041 taccgtgttg atgccagtga agaaaacatc cagatgtcca acctgcacga aggtcagagc
    47101 ttcttcgaaa tgctgggtga atacattctg gcaggtttca aagttgccat tatcgttgcc
    47161 gcgatgctga tcggctttat cgccctgatc gccgcgctga acgcactgtt tgccaccgtt
    47221 actggctggt ttggctacag catctccttc cagggcatcc tgggctacat cttctatccg
    47281 attgcatggg tgatgggtgt tccttccagt gaagcactgc aagtgggcag tatcatggcg
    47341 accaaactgg tttccaacga gttcgttgcg atgatggatc tgcagaaaat tgcttccacg
    47401 ctctctccgc gtgctgaagg catcatctct gtgttcctgg tttccttcgc taacttctct
    47461 tcaatcggga ttatcgcagg tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt
    47521 tctcgcttcg gtctgaagct ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca
    47581 atcgcagcac tggtgctgta agaccataca taaaaaagcc ggggataatt ccccggtttt
    47641 tttacgcctg ttaatcagct aatggctgcg ggcgaccaat caaataccct tgcagatatt
    47701 gcaccccgag cttatgcaat agcgcctgct gctgttgcgt ctcgacaaac tccgcgacca
    47761 cactcaatga cttcgctttc gccagatcgg taattgatct cacaatcatc gcatccagcg
    47821 tattcgtgac aatatctttc acaaagatgc catcaatttt gatgatatca gcctgcaaac
    47881 gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc atcaatcgca atccggaaac
    47941 caaacttatg cagctgctcg atgttgtaca tgctgctttc tgcgttagaa aacgcctgct
    48001 cctcggtgat ctcaagaatg accgcctgcg gggagatgtg ataacgctta aacagacgaa
    48061 taatccgccc ggcaatattc ttttgcagca gcgtgagcgg cattaaattg actgaaaagc
    48121 gcggtccttt tttgtcgcaa gggtgtgttg ccagccactt caacaaggat tccagcactt
    48181 gcaaatcaaa acgcgcgcta aggttaaact gagcaataag gggcagaaac ttatccgggg
    48241 tcataatgcc gccgtcatat ttcagtcgcg cgaggatctc atcataaccg tcaccctctt
    48301 tgttgcgaat tggctgggcg tagagcagca aatcaccctg atctaacgcc gtgcgaatgg
    48361 tattcagcaa tagcacctgt ttagtggtct gcccggaaac catctcctct ctgctatcca
    48421 gcgccagcac atgatgatgt gcgcaggatt gctccgccag ccagcttaac tgccccaaca
    48481 agggttgcag ggtttcctga tttccatcaa aacgccccca ggcagcacca tagcccatat
    48541 ccagcccggt attgttccag tgaatttgcc gactattcag gatgttaacc atatgctgga
    48601 gtcgcccttc cgtttccggc ccgctcagca ctaacagcag ttcactaccc ggcaattgat
    48661 acaacttttc gttttcctgc atcaacggca gcagcgtacg gcaaattgag cggatacagt
    48721 gaacgcgcat cattaagccg taatgacgac tcataaactc aagattatca atgcgcaggc
    48781 agcaaaaact cttgcccgcc tcttgctccg gcgcttgttc caacgcacga aagttgggta
    48841 ggagcgttaa cggatctgtc agcgcctgca aatgccagcg gcgattaagc cattcactgc
    48901 gatgataaat gcgcaccata tagagcaggc aaacgctaaa ggaaatcagc accgcaagaa
    48961 taaatgccag cgaatattcg gtttccaccc cttgcaaaaa gttctggttg taattcagca
    49021 ggcaaagcgt tgaaacagcc caggtgagat ttaaaaacgg atagcgaagc ttaccgaccc
    49081 cgagggtaaa gatgatgaag aaaacgggta ccaagtaacc ggcaataaag tcgttttcat
    49141 aaggtgtgca caacagaagt agcagcacgc ttaacgctgc cagccagctt aaggtaaatg
    49201 cgcgtttctc tttgcccaac gacggagcga tatccctgcg ccacaatatc tgcgcaaagt
    49261 ggggacttac aatcatgcga gtgagatagt agaaaagcat gttgtaaatc agcacagcgg
    49321 tgaaaaggct tagcaaatca acgaccgtga aaatggcatc cgcatcgccg aaaaaggtgg
    49381 atatctttag cgggaaatca aagaagctcc ccacaagata catgctgcat ttgatgccga
    49441 tcggcgtcac caaaccaagc cagaacagac gttgccagac atatcggctg gtcaatccat
    49501 aacgccaacg cgtccccagc tgccagcgta atattgcgca ggcacacaac acgacaaacg
    49561 tctggctacc gagcatgacc gcagtctgta aaaaagagag atgaaagttc cacagattgg
    49621 tgcaaaacat cccgcataaa acgggaacga caccgcgcca gccaaagata aacaagattg
    49681 acagcattac gcacagtggc atccacgcca gaaagatata actggaatgg ataatggcaa
    49741 gtggcgaaat aaaacgggat agctgaataa gtaccacggt gagcgtaaac gctagtgtga
    49801 atatcttgat attttttatc aggttatgct ccacaaacat gagtacacta ctctcactga
    49861 tcggggtttt caggcacaca aataataagt ttattcgcat cgcgaagcaa acaggggcca
    49921 gcctgttgcc gatttttcat ccaatgatgc tttcaccagg cggcgctaac ccggacgtag
    49981 actgtattac aaaagcggca aaaagcagag acaaaaaacc cccgctttgc agcgagggtt
    50041 ggaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc
    50101 tctcccagct gagctatagc cccacgatgc gtttacgtac caaatttgct gggtgcaaaa
    50161 tttggtggag ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc
    50221 cagctgagct atagccccgt cacgtaaagc ttgtcgagtt gacgggcggc atcatatgaa
    50281 ttccgcccga atgtgtcaac ggcaaattgc aacgtgtagt ttcaatcgct gaaaaatcag
    50341 gcaaatgaac aattttggaa aaccgctcgc attcagtagt tattcatgtc acggtttcct
    50401 gtaaagtggt gttataaaat gaactactaa tagacccaca tacattcagg gaattgttat
    50461 gttcaaggag aggatgacac cagatgaact tgccagactg accggctata gccgccagac
    50521 cattaataaa tgggtacgca aggaaggctg gacaacgtca ccaaaacctg gcgtccaggg
    50581 tggcaaagcc cgactggttc acgtcaatga acaggttcgt gaatatattc gcaatgctga
    50641 acgtccagaa ggtcagggag aagcgcctgc cctttctggt gatgcgccgc ttgaagtatt
    50701 gctggtgaca ctggcgaaag agatgacgcc agttgaacaa aaacagttta catccttgct
    50761 tctgcgggaa gggattatcg gattgttaca acgcttaggg attcgcgata gcaaataata
    50821 tgaaaagatt acgcaacaaa atgaccaccg aagaactggc tgaatgcctc ggtgtggcta
    50881 aacaaactgt taaccgttgg atcagagaaa aaggctggaa aacggaaaaa tttcccggcg
    50941 tgaaaggcgg tcgtgccaga cttattctgg tcgatacgca agtttgcgag tttattcaga
    51001 acacgccagc cttccataac acaccaatgt tgatggaagc tgaagagaga attgctgaat
    51061 acgctcctgg agcacgcgcc ccggcgtatc gacaaatcat caacgcgata gataatatga
    51121 cagacattga gcaggaaaaa gtcgcgcaat ttttgtcacg tgaaggaatt cgcaatttcc
    51181 tcgcccgtct cgatattgac gaatcagcat aaataaaaaa cggcaggata ttatctcctg
    51241 ccgtttatct ttttacacgc taattactgc tgattttcgc gttcagcaat aaaatccagc
    51301 gctttgttga tacgctcgat actgcgggtc ttaccaattg cgtgaacggt aacatccagt
    51361 gctggagact gccccgcacc ggttacggcg acacgcagcg gcataccaac tttacccata
    51421 cccacttcca gctcatccgc cgtcgcctga atagcgtgat gaacgttttc agcggtccag
    51481 tcagtaatcg cggccagttt gtcacgaacc acttccagcg gctgacgcgc taccggacgc
    51541 agatgttttt tcgccgcatc ggcatcgaac tcagcaaaat cttcgtagaa ataacggcag
    51601 ctctgtgcca tctctttcag cgtcttgcag cgttcgccca gcagtttcac cagatcagcc
    51661 agctgcgggc cgttacgggt atcgatattt tcctgctcaa tgtgccactg taagtgagta
    51721 gcaacatact ccggcggcag cgcgttaatg tagtgatggt tcagccacag cagcttgtcg
    51781 gtgttgaacg cactggcaga tttgctgacg gcattcaaag tgaagtattt gatcatctct
    51841 tcacgagtga agatttcctg atcgccgtgg gaccagccca gacgcaccag atagttcagc
    51901 agtgcttctg gcaaataacc gtcatcacga tactgcatta cgctgactgc cccgtgacgt
    51961 ttggacagtt ttttaccgtc atcgccattg atcatagaaa cgtgcgcgta aaccggcacc
    52021 ggcgctttca gggccttaag aatgttgatc tggcgcggcg tgttgttgat atggtcttcg
    52081 ccacggataa cgtgggtgat ttccatatcc cagtcatcga caaccacaca gaagttatag
    52141 gttggggaac catcggtacg gcggataatc agatcatcca gctcctggtt gctgaactcg
    52201 atcggaccac ggatctgatc gtcaaaaaca acagaacctt cctgcggatt agcaaaacgc
    52261 acgacacacg gttcatcatc agcatgatgc tcatggctgt ggcggcagcg accgtcataa
    52321 cgcggcttct cacctttcgc catttgctct tcgcgcagcg cttccaggcg ctctttagag
    52381 caatagcatt tataagcagt gccctcttcc agcatctgat cgatcaccgc attgtagcga
    52441 tcaaaacgtt tggtctggta gtacggacct tcatcccact ccaggctcag ccagttcatg
    52501 ccatccataa tggcttcgat agcttccggc gtggaacgct caagatcggt gtcttcaata
    52561 cgcagcacga actcaccgcc gtggttacgt gcaaaaagcc aggagtaaag agcagtacgc
    52621 gcgccgccaa cgtgcagata gcctgttggg cttggcgcga agcgagtttt gattttcatg
    52681 aaatggcctt acgtttagaa agatgccgac aaccggcaaa tcctggaaaa attgagtggg
    52741 cgatattcta tcactcccgc ctgattcctc aatgtagttc gggttgcaac cttacgcatt
    52801 attgttttcg ttgacaaatt gcgcaacacg gtctgttttg cgatcatctt gcttaaattt
    52861 acgacgaacg aacaatttct ttagaaaatg cgttgactca ttttgaactc tccctataat
    52921 gcgactccac acagcggggg tgattagctc agctgggaga gcacctccct tacaaggagg
    52981 gggtcggcgg ttcgatcccg tcatcaccca ccaactactt tatgtagtct ccgccgtgta
    53041 gcaagaaatt gagaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg
    53101 gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg tcgttagctc agttggtaga
    53161 gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccaatgtaaa
    53221 aaaagcgccc taaaggcgct tttttgctat ctgcgatact caaagattcg aagctgccgc
    53281 aggttcgagt tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga
    53341 gcgaagcgag tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt
    53401 tgctatctgc gatttgcgaa attgcctgat gcgcttcact tagcagacga ctatttccgg
    53461 caattcctgt ctcctcacct actgtgtcaa tgcagccaac agcttaacca tcgcgggcgt
    53521 cacctgctgt gtttcataaa caatatataa atctgcaggg atgcgctgtt tgagcggacg
    53581 gaaaatgaca cctggccagt tcatttgtgc gtagctgtcc gctatcaatg tgataccaat
    53641 acccatactg accatggcga gtaccgtttg cggttcatta acttcgcgaa taacaaccgg
    53701 tgaaaatccc acctgctggc aaactcgctg caaaaaatcc cagtcagtgt acacgggcgg
    53761 cattgtaaca aaatactcgt cacgtagcgc ttccagcgga acggtggaaa atgatgagag
    53821 atgatgctct tcaggcatcg ccaccagaaa cgccgattca tgcaaccgta agctggtaaa
    53881 acctgtcggt ggttctgtcg ccattcgcca gatcccggca tcgagttcgc gtcgttccag
    53941 cagggccatt tgcatcgcgg gcatcttttc gcgaaaaaga acttcaacgt taggattttc
    54001 cctgaggaat cgccgcataa ccgggcgcat ccgtccccac atagccgttc ccactacgcc
    54061 gagttcaatc cgccctgctt ctccccgacc tatttgttca acccgagcca atacattatt
    54121 agcattcacc agcaatcgac gcgattcttc catcaagatt ttgcccgcgt gtgtcagtac
    54181 gacgctgcgc gaatggcgaa taaaaagctg cgtgccgagt tgattttcca gctctttaat
    54241 atgaatactg agcggaggct gagacatatt taaacgcgct gctgcgcggc caaaatgcaa
    54301 ctcttccgct acggcaagaa aataacggag caacttaaga tctgttctgt atacgcgttc
    54361 cataattaag tgctccatat aataaataca tcgtattaaa ttatcatggc ataaagtatt
    54421 tcaccaccag atatccgaca tacataaaca caatggataa tatacctggt aatacatttg
    54481 gtacaggaat aggcaagcgc acgattgtaa acaaagcacc aatgccaaaa ccaacgccgg
    54541 aagaaaataa aatatctttc actaattaac ctttatcata aaagcagctc tgaagagcag
    54601 agctgcgaat ccttttaatg agccaccgct cgatgcttta tcttttcagg gtcatgatta
    54661 tatttaaacc caaagaaaaa tatcactgcg agaaaaagag catatcctgc aaacaccagc
    54721 cagatagttt gccagtcttt tacgccatcc accgaaaagt aatctactgc catgccactc
    54781 agaatcgagc caacccatgc gccgacacca tttaccatgg tcataaagag cccctgcgcg
    54841 ctggcacgaa tgctggaatc aacttcctgt tcgacaaata ccgaaccaga aatattgaag
    54901 aaatcgaatg cacagccata aacaatcatc gacagcagca gcaaaataaa tccggttgtt
    54961 gacggatcgc cataggcgaa gaagccaaag cgcagcgtcc aggccaccat actcatcagc
    55021 atgacggttt taatgccaaa tcgctttaaa aagaatggga tagtcagtat aaagcccact
    55081 tctgccatct gtgaaactga cagtaaaatg gagggatatt tcaccacaaa actgtcagca
    55141 aactccgggt tacgggcgaa atcatgtagg aacggattac caaaaacgtt ggtaatttgc
    55201 agtaccgcac ccagcatcat ggcaaagagg aaaaagatgg ccatgcgtgg atttttaaac
    55261 agcacgaagg catccagacc cagcttgctg gcaagcgatg tggtcgcttt tttctccgca
    55321 accggaatct tcggcaaagt cagcgcataa gccgacagca gcaatgacgc accggacgcg
    55381 atatacagct gcagactact caattccaga tgcagcaggc ttactgccca catcgcgaca
    55441 atgaacccca ccgtaccaaa aacgcgaatg ggcgggaaag cggtcaccgg gtcaagccct
    55501 gcctgggcaa gacaggaata agagacgctg ttcgataacg caatagtcgg cataaacgcc
    55561 atcgcattga ctaacatcac ccaaaacatc atatccggat cagttacgga tgccgcataa
    55621 aaaagtacgc ccgcacacac caggtgacac agcatgtatg cacgttctgc gcgcagccat
    55681 ttgtctgcga tgatccccat tataccaggc ataataatcg cggcgatccc tttggaactg
    55741 taaaccatgc caacattagc gccggtgaaa tgaagggtat taatcatgta agagccgagg
    55801 gtaaccagcc agctccccca gataaaatat tgcaaaaagg acattacctt taagcgcatc
    55861 gcgatgctca taatcgtttc cttgtcgcat catttggcct cacccgcgag gccgtttctt
    55921 attgtttttc tcaggcaatt ttgcgcagaa agccacaaat aaggttgatg aagttctgct
    55981 tagagagctc cgcagccgcc agcgtttgcg catgtgacaa tttcacatcg cttaaacctt
    56041 ccgccatatt ggtaatcgca gagaccgcaa cgactttaag gtcgcaatgg cgagctgaaa
    56101 taacctcagg caccacagac ataccaacaa catccccacc aataatttgc atcatgcgaa
    56161 tttccgccgc agtctcgaaa ttcggccccg gatacgagac gaacacgccc tccgtcagag
    56221 ggaaaccctc ttctttcgcc actttttgta acagtgcgcg gtattccgca tcgtaggcat
    56281 ttgccagtga gaagaagcgc tctccaaaac gatcatcgtt aagacccacc atcggcgtac
    56341 cgggcatggt gttgatatga tctttcaatg cgaccaggct gcctgccccc acttccggac
    56401 gcagcgagcc tgccgcattg gtgcagaaca gtagctcgca gcccagcagc ttaaaggtac
    56461 gaattgcgtc agtcataatg gtcattccac gaccttcgta gaaatgtccg cgacctttca
    56521 tacataccac cggtacgccc tggagatggc ccagcactaa ctcccccgca tgaccatgta
    56581 ccgtacttac cgggaagccc ggcagctttt cgtaggaaat tgcgacagcg ttctcaatct
    56641 gatcggccag cgcgccgagc ccggaaccta aaataaaggc cactcgtggc gtgaaatcag
    56701 gtttataagt cttgataata tctatgcaaa acagcgggtt atgagaaaat tgaacctgag
    56761 acatacatat cctttttctg tagggtggaa tctaacgcct ttttatagcg aggttgtccc
    56821 ggctcttacc aatacacttt tctcacgcat tcaatagcga ttcgatatcg atggatttat
    56881 atcttttctc tattaatcca cggtaaaaac tgtattggcg atattaattc ctttgcatta
    56941 acaatatgtc gtttttagca aaccacttta ttaagcaatc tactaataac gatcggcata
    57001 gggaattatg ctatgaaaaa acatctttta actctgacac tttcctctat attagcgata
    57061 cctgttgtat ctcatgcaga atttaaaggc ggttttgcag atatcggcgt gcattatctg
    57121 gactggacca gccggactac cgaaaaatcg tcgaccaaat cacacaaaga tgattttggt
    57181 tatcttgaat ttgaaggtgg cgctaacttt agttggggag aaatgtacgg gttcttcgac
    57241 tgggaaaact tctataacgg tcgccataat aaaccaggta gcgagcagcg ttataccttt
    57301 aaaaatacca accgtattta cctgggtgac accggattta atctctactt gcatgcgtat
    57361 ggcacctacg gttctgcgaa tcgcgtgaat ttccacgacg atatgttcct gtacggtatc
    57421 ggctacaatt tcaccggcag cggttggtgg ttcaaaccgt tctttgctaa gcgttataca
    57481 gatcaaacct attacactgg cgacaacggc tatgtcgccg gctgggttgc aggttacaac
    57541 tttatgctgg gcagcgagaa attcactctt accaactgga acgagtacga gtttgaccgt
    57601 gacgctacct atgcagcggg taatggcggt aaagaagggc tgaatggcgc agttgcactg
    57661 tggtggaatg caacatcaca cattactacg gggattcagt atcgatatgc ggatgacaaa
    57721 ctgggcgaag atttttacca ggatgcgata atctactcca tcaaatttaa cttctgattg
    57781 attactgccg gggctcccgg cagtatgaat tcactctata tctgatacaa cggatcccct
    57841 tcccgaccag ccattagcgc attgccctgg tctcgtacca cgtcccataa cgcttcagcg
    57901 gcggtggaaa gtgaacggtt cttcctgcgc accagcatca actggcgttc aacaaccggc
    57961 gtaatgcgct ttaccaccag tgggctacct tcaggcaatg gtagcgccag tgcgggaaga
    58021 atactgatgc cgatgcctgc ggctaccatc gggaacagcg tcgccggatg tccaatctcc
    58081 tgcacaatat tcgcctgaat accattgcgc gccagcgctg cgtcaatcag cggtcggctg
    58141 cctgacgcgt aatcctgcaa caccaatttc gctccctgta atgcctgcca gggtacataa
    58201 tcttcgacgg ccaaagcgct atcgcgatgg caaagcagaa agaaaggctc ggaaagaatc
    58261 gcttcacatt gcagatcgcc cactgggcct ggatcgatga cgatgccaaa gtcgacatct
    58321 ccctggcgaa tactttccat cacccactgc tgcggacgat cgtgcaggac aaactgaata
    58381 tctggatagc ggcgatggct ttcagcaatg cattgcggaa taagatgcgc ggaaatggtc
    58441 tggctggcag cgacccgaac ttttccgctc agttgttgcc ccatacgccc ggtatcgcgc
    58501 aacgtgctgt tcagttcatc cagcagtcgc tcaagacgca aagccagctg ctgccctgca
    58561 tctgtaagca ccacttcacg cgtggttctg tccagcaggc gaacaccagt atgattttcc
    58621 agttccttca cactgtgact cactgccgac tggctcaggc cgatacgctc tcctgcacga
    58681 ctaaaacttt tctcctgcgc tactgtgacg aaaaccttta attgttttaa agaataattc
    58741 atctgttttc ttcatgaatg gatgcaataa atcaatttta tttctcaaac tggaagaagc
    58801 acaatagaac catcgatcat ctggagtctt tatgaaactt tttcgtatcc tcgatccttt
    58861 caccttaacc ctgatcacgg tggtgttgct ggcctctttc tttccggcca gaggcgattt
    58921 cgtccccttc tttgaaaatc tgaccaccgc agcgattgcc ctgctgttct ttatgcacgg
    58981 cgcgaagttg tcgcgtgagg cgattattgc tggcggtggt cactggcgac tgcatttgtg
    59041 ggtaatttgc agcaccttcg tgctgtttcc gattctgggt gtactgtttg cctggtggaa
    59101 accggtaaat gtcgacccga tgctctactc cggttttctc tacttgtgca ttctcccggc
    59161 taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc ggtaacgtcg cggcggcggt
    59221 ttgttctgcg tcagcatcca gcctgctggg gattttcctt tcaccattgc tggttggtct
    59281 ggtgatgaat gttcacggtg cagggggcag ccttgagcag gtcggtaaaa ttatgctgca
    59341 actgctgctg ccgtttgtgt tggggcatct ttcccggccg tggattggtg actgggtgtc
    59401 gcgcaataaa aaatggattg cgaaaactga ccagacgtcc attctgttgg tggtttatac
    59461 agcattcagc gaagccgtcg ttaatggtat ctggcataaa gttggctggg gatcattgct
    59521 gtttatcgtg gtggtcagct gcgttcttct ggctatcgtg attgtagtta acgtctttat
    59581 ggcacgccga ctgggcttca ataaggcaga tgaaattact atcgtctttt gtggttcgaa
    59641 aaagagtctg gcaaatggca tcccgatggc aaacattctg ttccccacat cggtgatcgg
    59701 tatgatggtg ctgcccctaa tgattttcca tcagatccaa ttgatggtct gtgcggtgct
    59761 ggcgcgtcga tacaaacgcc agaccgaaca gttacaggca cagcaggaaa gcagcgccga
    59821 taaagcttaa agcggacgct tcaggggctg gaccagttgc gtcagcccct cggttttaat
    59881 cagcagcgtg atttgcatta gctcaccgag cttccctgcc gggaattcat ctttgcgggc
    59941 aaaccacagc agatactcct ccggcaggtc gattaagcga cgccctttat atttgccaaa
    60001 cggcattatc gtattggcta tttcaatcag ctgctctttt tccatctcag ctacccagca
    60061 aacgcagcat ttccgcttcg tcgatgactt caatgcccag ttcctgcgct ttcgccagtt
    60121 tagaacctgc cgcttctccg gcaatcacca gatcggtttt cttcgacacg ctgcccgcga
    60181 ctttcgcgcc cagttcaacc agtcgagctt tagcgtcatc acgcgacatc tggcttaagc
    60241 tgcccgtaag caccacggtt ttaccagcaa acgggctgtc aatctcttcc gcgttgataa
    60301 cgatcggcgc aggccagtga acaccttccg ccaacagctc gctgatgaca ttgcggttgc
    60361 tttcttcggc aaagaagttg tgaacgtggg atgcaacgac aatgccaaca tcaggcacct
    60421 tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag cgtgccgaaa tatgccgcca
    60481 gacctgctgc ggtggcctcg ccgacttcac ggatgccaag tgcatagagg aagcgagcaa
    60541 aggtggtttc tttcgctttt tccagcgcgt taaccacgtt ttgtgccgat tttggcccca
    60601 tacgctccag tccggtcagt ttgcctgcgg tgagtttgaa cagatctgcc ggagtatgga
    60661 catattcttt ttcaaccagc tgatcgatga ttttgtcgcc cattccgtca acatccatcg
    60721 cacggcggga aacaaagtgt ttcagcgact ctttacgctg cgcaccgcaa atcaggccac
    60781 cggtacagcg ggcaaccgct tcaccttcca cacgctcaac gtcagaacca cataccggac
    60841 aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg ttcagaaagc acgacgttaa
    60901 ccacctgcgg gatcacgtcg ccagcgcggc gaatcaccac tttatcgcca atgcgtaaac
    60961 caagacgttc gatttcatcc gcattgtgta aggttgcgtt actcaccagc acgccagcaa
    61021 catggacagg ttccagacgc gcaacaggcg taatcgcgcc agtacgccca acctgaaact
    61081 cgacgtcacg cacaaaggtc atctgctcct gcgccgggaa tttaaacgct accgcccagc
    61141 gcggggcacg cgcgacaaag ccaagctgct cctgctgttc cagtgagttg accttaatca
    61201 ccacgccgtc gatatcaaag cccagcgtcg ggcggtcttc ttccactttg tgatagaacg
    61261 ccagcacttc ttccgccgat tcacaaagcg ttacccgatc gctgaccggc aacccccact
    61321 ttttaaattg cagtaaacgg ccaagatgag tatccggcag ctcgccacct tccagaacac
    61381 caacgccata gcagaaaaaa gtgagcggtc gcttcgctgt aatacgcgga tcaagctgac
    61441 gcagtgaacc agctgccgca ttacgtgggt tagcaaaaac tttcccgccc gtgcgtcgcg
    61501 catcttcgtt aatcttttcg aaccccgcct gcggcaggaa cacttcacca cgcacttcca
    61561 gacgcgccgg gatattctct ccgtgcagct tcagcggaat agcgcgaata gtacgcacat
    61621 tagacgtgat atcttccccg gtggtgccat cgccacgggt cgcggcactg actaaaacgc
    61681 cattttcata cagaatactg acggcaaggc catccagctt cagctcacag caccaggtga
    61741 ctttctcgtt gtttttcaga cggtcctgca cacgtttgtt gaaagcaaga aagctttctt
    61801 catcaaaaac gttatccagt gacagcattg gtacttcatg gcgtatctgg ctgaaagccg
    61861 ccagcggcgc agcgcctaca cgttgggtag gcgaatcagg cgtaatcagt tctggatgtt
    61921 tggtttccag ctcgcgcagt tcgcgcatca gcctgtcgta ttcagcgtcg ggaatttccg
    61981 gcgcatccat cacatgataa agatattcat gatggcgaag cgtcgttcgc agttctgtca
    62041 gttgttgttc gattgattcc atatcgcacc atcaatgcta aaaacccccg acaagcgggg
    62101 gttcgaagag gagttaattt gccttaagtg tatcaggcgt tggcgtcttt gacttcgcgg
    62161 atgatgtcct ggtactcgcg caatttctgc ggagtcatca tacggcgctg atcgtcaagc
    62221 acgacaccgc ccacttcatc ggcaatatgc tgcgcagatt gcagcatcag cttgaagttc
    62281 tgcagctcgt caccgtaaga cggtacctgc ataaagatgg tgacacccgg agtagtgaaa
    62341 tccttcattt caggatcaaa ggttcccggt ttcaccatat tcgccaggct gaataacgcc
    62401 gggccgctgc catccgggct aagatgacga tggtaaatat tcatatcgcc aaaaatgaag
    62461 cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta gctcgctacc gtgatgcgcc
    62521 gcgacgttca tgataatcac cgcttctttg cgcttcggtt tatccataac tggagccggt
    62581 tccgctacag gctcaggctg tggtgccgct acgggttctg caggctggaa agcctgttgt
    62641 gccggttgcg gtgctgaatg cacaggctgc ggcgctggcg cgacctgtgg cgaaactggc
    62701 tgctgcaacg gctgttcagg ctgcggctga taggcaggct gctgcaccgg ctgcgctgga
    62761 cgcggagcat gttgcggcgg tacctgcgct tcaggcggct gctggaccgg ttgacgcggc
    62821 tgcgcagacg cataaggcgg ttggtactgg tgttgcggcg acggacgagc agcctcatgc
    62881 tcctgagcgt tagccggggc atgattcacg cggtgaacac gaacctcacc aacgccctca
    62941 tcatcttcga catcctcgtc ataagaatcg tcgtcacgtt ttgacttcat tcgttttaat
    63001 ggccgatcgc ggaacataga agatcgttct ttacggctgg tccagaaacc atgtaccagt
    63061 aaagcgatta tggcgatcgc gccaacaatg attaatatca gacgcaaatc ctgcatcatt
    63121 atattctctg ttgttctaac accttgccac cacggcaaac atttactcac taagagtatt
    63181 tgccgattac ctcaagtgca agtgcactat taactttcac agcacaaaga tagatgaaat
    63241 cgtgcttttt gctgtttttt cgaacatatc ctaactgtcc attgcgcaat tacccggtaa
    63301 aatacgcaga attttcctgg gattggtcaa aaggagctca tcctgattat ggtttcatca
    63361 ttcacatctg ccccacgcag cggtttttac tattttgcgc aaggctggaa gctcgtctcg
    63421 caacctggga ttcggcgttt cgttatttta ccgctgctgg tcaatatttt gttgatgggg
    63481 ggcgcattct ggtggctctt tacacagctc gatgtctgga tcccgactct catgagttac
    63541 gttccggact ggctacaatg gctgagttat ctgttgtggc ctctggctgt catctctgtg
    63601 ctgttagtgt ttggctattt cttctccacg attgctaact ggattgccgc tccgtttaac
    63661 ggtttattgg ctgaacaact ggaagcacga ttgactggcg ctacaccgcc agataccggg
    63721 attttcggta tcatgaaaga tgtgccgcga atcatgaaac gcgaatggca aaaatttgcc
    63781 tggtatctgc cgcgcgcaat tgtattgcta attctttact taatccctgg tattgggcaa
    63841 accgtcgcgc cggtactgtg gttcctgttt agcgcctgga tgttagccat ccagtattgc
    63901 gattacccct tcgataacca caaagtgccg tttaaagaga tgcgcaccgc cctgcgcaca
    63961 cgcaaaatca ccaatatgca gtttggtgct ttaaccagcc tgtttacgat gatcccgctg
    64021 cttaatctgt tcatcatgcc cgttgccgtt tgtggcgcga cggcgatgtg ggtcgattgc
    64081 tatcgcgata aacacgcgat gtggcggtaa caatctaccg gttattttgt aaaccgtttg
    64141 tgtgaaacag gggtggctta tgccgcccct tattccatct tgcatgtcat tatttccctt
    64201 ctgtatatag atatgctaaa tccttacttc cgcatattct ctgagcgggt atgctacctg
    64261 ttgtatccca atttcataca gttaaggaca ggccatgagt aagatttttg aagataactc
    64321 gctgactatc ggtcacacgc cgctggttcg cctgaatcgc atcggtaacg gacgcattct
    64381 ggcgaaggtg gaatctcgta accccagctt cagcgttaag tgccgtatcg gtgccaacat
    64441 gatttgggat gccgaaaagc gcggcgtgct gaaaccaggc gttgaactgg ttgaaccgac
    64501 cagcggtaat accgggattg cactggccta tgtagctgcc gctcgcggtt acaaactcac
    64561 cctgaccatg ccagaaacca tgagtattga acgccgcaag ctgctgaaag cgttaggtgc
    64621 aaacctggtg ctgacggaag gtgctaaagg catgaaaggc gcaatccaaa aagcagaaga
    64681 aattgtcgcc agcaatccag agaaatacct gctgctgcaa caattcagca atccggcaaa
    64741 ccctgaaatt cacgaaaaaa ccaccggtcc ggagatctgg gaagataccg acggtcaggt
    64801 tgatgtattt attgctggcg ttgggactgg cggtacgctg actggcgtca gccgctacat
    64861 taaaggcacc aaaggcaaga ccgatcttat ctctgtcgcc gttgagccaa ccgattctcc
    64921 agttatcgcc caggcactgg ctggtgaaga gattaaacct ggcccgcata aaattcaggg
    64981 tattggcgct ggttttatcc cagctaacct cgatctcaag ctggtcgata aagtcattgg
    65041 catcaccaat gaagaagcga tttctaccgc gcgtcgtctg atggaagaag aaggtattct
    65101 tgcaggtatc tcttctggag cagctgttgc tgcggcgttg aaactacaag aagatgaaag
    65161 ctttaccaac aagaatattg tggttattct accgtcatcg ggtgagcgtt atttaagcac
    65221 cgcattgttt gccgatctct tcactgagaa agaactgcaa cagtaatgcc agcttgttaa
    65281 aaatgcgtaa aaaagcacct ttttaggtgc ttttttgtgg cctgcttcaa actttcgccc
    65341 ctcctggcat tgattcagcc tgtcggaact ggtatttaac cagactaatt attttgatgc
    65401 gcgaaattaa tcgttacagg aaaagccaaa gctgaatcga ttttatgatt tggttcaatt
    65461 cttcctttag cggcataatg tttaatgacg tacgaaacgt cagcggtcaa cacccgccag
    65521 caatggactg tattgcgctc ttcgtgcgtc gcgtctgtta aaaactggcg ctaacaatac
    65581 aggctaaagt cgaaccgcca ggctagactt tagttccaca acactaaacc tataagttgg
    65641 ggaaatacaa tgttccagca agaagttacc attaccgctc cgaacggtct gcacacccgc
    65701 cctgctgccc agtttgtaaa agaagctaag ggcttcactt ctgaaattac tgtgacttcc
    65761 aacggcaaaa gcgccagcgc gaaaagcctg tttaaactgc agactctggg cctgactcaa
    65821 ggtaccgttg tgactatctc cgcagaaggc gaagacgagc agaaagcggt tgaacatctg
    65881 gttaaactga tggcggaact cgagtaattt cccgggttct tttaaaaatc agtcacaagt
    65941 aaggtagggt tatgatttca ggcattttag catccccggg tatcgctttc ggtaaagctc
    66001 tgcttctgaa agaagacgaa attgtcattg accggaaaaa aatttctgcc gaccaggttg
    66061 atcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc atcagcccag ctggaaacga
    66121 tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga agccatcttt gaagggcata
    66181 ttatgctgct cgaagatgag gagctggagc aggaaatcat agccctgatt aaagataagc
    66241 acatgacagc tgacgcagct gctcatgaag ttatcgaagg tcaggcttct gccctggaag
    66301 agctggatga tgaatacctg aaagaacgtg cggctgacgt acgtgatatc ggtaagcgcc
    66361 tgctgcgcaa catcctgggc ctgaagatta tcgacctgag cgccattcag gatgaagtca
    66421 ttctggttgc cgctgacctg acgccgtccg aaaccgcaca gctgaacctg aagaaggtgc
    66481 tgggtttcat caccgacgcg ggtggccgta cttcccacac ctctatcatg gcgcgttctc
    66541 tggaactgcc tgctatcgtg ggtaccggta gcgtcacctc tcaggtgaaa aatgacgact
    66601 atctgattct ggatgccgta aataatcagg tttacgtcaa tccaaccaac gaagttattg
    66661 ataaaatgcg cgctgttcag gagcaagtgg cttctgaaaa agcagagctt gctaaactga
    66721 aagatctgcc agctattacg ctggacggtc accaggtaga agtgtgcgct aacattggta
    66781 cggttcgtga cgttgaaggt gcagagcgta acggcgctga aggcgttggt ctgtatcgta
    66841 ctgagttcct gttcatggac cgcgacgcgc tgcccactga agaagaacag tttgctgctt
    66901 acaaagcagt ggctgaagcg tgtggctcgc aggcggttat cgttcgtacc atggacatcg
    66961 gcggcgacaa agagctgcca tacatgaact tcccgaaaga agagaacccg ttcctcggct
    67021 ggcgcgctat ccgtatcgcg atggatcgta aagagatcct gcgcgatcag ctccgcgcta
    67081 tcctgcgtgc ctcggctttc ggtaaattgc gcattatgtt cccgatgatc atctctgttg
    67141 aagaagtgcg tgcactgcgc aaagagatcg aaatctacaa acaggaactg cgcgacgaag
    67201 gtaaagcgtt tgacgagtca attgaaatcg gcgtaatggt ggaaacaccg gctgccgcaa
    67261 caattgcacg tcatttagcc aaagaagttg atttctttag tatcggcacc aatgatttaa
    67321 cgcagtacac tctggcagtt gaccgtggta atgatatgat ttcacacctt taccaaccaa
    67381 tgtcaccgtc cgtgctgaac ttgatcaagc aagttattga tgcttctcat gctgaaggca
    67441 aatggactgg catgtgtggt gagcttgctg gcgatgaacg tgctacactt ctgttgctgg
    67501 ggatgggtct ggacgaattc tctatgagcg ccatttctat cccgcgcatt aagaagatta
    67561 tccgtaacac gaacttcgaa gatgcgaagg tgttagcaga gcaggctctt gctcaaccga
    67621 caacggacga gttaatgacg ctggttaaca agttcattga agaaaaaaca atctgctaat
    67681 ccacgagatg cggcccaatt tactgcttag gagaagatca tgggtttgtt cgataaactg
    67741 aaatctctgg tttccgacga caagaaggat accggaacta ttgagatcat tgctccgctc
    67801 tctggcgaga tcgtcaatat cgaagacgtg ccggatgtcg tttttgcgga aaaaatcgtt
    67861 ggtgatggta ttgctatcaa accaacgggt aacaaaatgg tcgcgcctgt agacggtacc
    67921 attggtaaaa tctttgaaac caatcacgcg ttctctatcg aatctgatag cggcgttgaa
    67981 ctgttcgtcc acttcggtat cgacaccgtt gaactgaaag gcgaaggctt caagcgtatt
    68041 gctgaagaag gtcagcgcgt gaaagttggc gatactgtca ttgaatttga tctgccgcta
    68101 ctggaagaga aagccaagtc taccctgact ccggttgtta tctccaacat ggacgaaatc
    68161 aaagaactga tcaaactgtc cggtagcgta accgtgggtg aaaccccggt tatccgcatc
    68221 aagaagtaat tcttgccgca gtgaaaaatg gcgcccatcg gcgccatttt ttatgcttcc
    68281 gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct gggtgtagcg catcacttcc
    68341 agtacgcgca accccgcacg gtgcaccgca tcgtttaacg ccttcccttt cagcaaacca
    68401 ctgataagct gagcacaaaa caggtcgcca gtccctttca ggtcggtttt tacccgtgaa
    68461 tgggaaatga cattcacgct gtcggcactg accaccacca cctgcatctc ctgattttct
    68521 tcattaccgg aggcgctggt aatcaccacc cattttaatg tgtctgaaag cagacttttt
    68581 gcggcagcaa tggcactgtc gagatcgcgg caatttttac cggtcaggat ttccagctca
    68641 aagatattgg gggtaattcc ctgcgccagc ggcagtaaat attgtcgata cgcctcggga
    68701 aggtcaggtt tgacataaat tccgctatca atatcgccaa tcaccggatc gaccatgatc
    68761 aataggtcag gatggtcttt gcgtagcgca gtcagccact cggcaaggat tttgatttgc
    68821 gatgccgttc ccatatagcc cgtggttaca gcgcgcagct ggcgcagcgc atcacgctcc
    68881 tgaagcgcac gcaaataacc gctaaaccat tcatccggaa tcgcaccacc gtagaaagtg
    68941 tcataatgcg gcgtattgct cagcaatacc gtcggcacgg caaaaacatt cagaccgttc
    69001 tgtttgatag caggcacggc aatgctgttg cccacgctgc cgtaaaccac ctgcgactgc
    69061 acggcgacga tatccgcctg cagcgccctg ctcttatcgt taaacaacaa caaactactc
    69121 atttaatttt ttctccttgc cgatgatcct catcgtattc caaccgaaac tttacctgat
    69181 tctggcagtc aaatcggcta tcacaaaaca aggataaggt aattcaatga agaaaatcat
    69241 ttgtctggtc attacactac taatgacact ccccgcttac gcgaagttaa ctgcccatga
    69301 agaagcccgc atcaacgcca tgctggaggg attagcacag aaaaaggatt tgatatttgt
    69361 gcgcaacggt gatgaacata cctgcgatga agcggtttct catctgcgtc tgaagctggg
    69421 caatacccgt aaccgcattg acactgccga gcagtttatt gataaggttg cttcgtcgtc
    69481 atcgattacc gggaagccgt atattgtgaa gatccccggt aagagcgatg agaacgcaca
    69541 gcctttttta catgcgctaa ttgcgcagac ggataaaacg gtgcctgcgc aataatctta
    69601 aatccccgcc ccctggctaa aatgctcttc cccaaacacc ccggtggaaa ggtagcgatc
    69661 gccacgatcg cagatgatcg ctaccaccac cgcaccaggg ttagcttttg ccacccgcag
    69721 tgctccggca accgcgccgc cggagctgac gccacagaat attccttccc gcactgccag
    69781 ttcgcgcatg gtgttttctg catcgcgctg atgaatatcc agcacctcat ccaccagaga
    69841 agcgttgaaa atccccggca gatattccgc aggccagcgg cgaatgccgg gaatactgct
    69901 gccttcttcc ggctgcaggc caacaatcgt tactggtttg gattgttcgc gcataaagcg
    69961 tgaaacgccg gtgatagtgc cggttgttcc catgctggag acaaaatggg taatgcgccc
    70021 gccggtttgc tgccagattt ccggcccggt agtggtgtaa tgggcgtaag ggttatcggg
    70081 attattaaac tgatcgagca gctttccttc gccacgattc gccatctcca gcgccagatc
    70141 gcgcgcacct tccatgccct gctctttggt gacaagaatc agttccgcac cataagcacg
    70201 catcgccgca cggcgttcct ggctcatgtt gtcgggcatc agcaatttca tgcgatagcc
    70261 tttcagcgcg gcaatcattg ccagcgcaat gccggtgtta ccactggtgg cttcgattaa
    70321 gacatcaccc ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg aaagtgccgc
    70381 acgatctttc accgaacctg ccgggttatt gccttccagt tttaaccaca cttcactgcc
    70441 gttatccggc cccattcgct gcaacttcac cagaggcgta ttgcctattg tttgttctaa
    70501 tgtactcacg atctctatcc atacgtggtg ttgcctgatg cgacgcttgc gcgtcttatc
    70561 aggtctacag gttacaaacc ttgccattaa aaagcccgga tcgcgggaag cgcctccggg
    70621 cgtttaacat tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc tcatctcggg
    70681 tttcgatacg ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg aataaacgct
    70741 cgccacgctg cggggcatcg tcgccatgca tcacgaccgt cagcggttcg ttgtaccacc
    70801 ccagcggctg caccactaat tgggtgtagt gacctttcgg gctggcttcc agtacctgta
    70861 ccggcagcgg cgaatcgaga ctggtacggc ggctgatatc cacttcccac gggcgcagga
    70921 agagatccac cggaccctga tacgcaggtg tgtagcccag cggccagcga tgcgcgccaa
    70981 catggaactg cccgccgcga atggttccct gcaggcggtt cacttcgccc ataaattcga
    71041 gtacaaaacg ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc tgttcaatat
    71101 tcccctgact catcaccact acacgatcgg ctacttcggt cgcctcttcc tggtcgtggg
    71161 tcacgaacac gctggtgaat ttcagctctt catgcagttg acgcagccag cgacgcagct
    71221 ctttacgcac ctgcgcatcc agcgcgccaa acggttcatc aagcagcaga atttgcggtt
    71281 ctacagccag cgcgcgcgcc agcgccacgc gctgtttctg gccgccggaa agctgtgccg
    71341 gataacgatc tgccagatga gcaagctgca ccatctccag caattttgtc actttcgctt
    71401 tgatggctgc ggcattcggg cgctcgcgac gtggcagcac cgtcaggcca aaagcgatat
    71461 tgtcgaacac cgtcatatga cggaacagcg cgtaatgctg gaacacgaaa ccgaccttgc
    71521 gatcgcgcgc gtgcaggcgg ctgacgtcgg tgccgtggaa gcgaatatgc ccgctggttt
    71581 gatgctccag cccggcgata atgcgcagca gcgtggtttt cccggaaccg gacggcccca
    71641 gcaacgcgac catctgacct gaaggaatat ccagtgagat atcgttcagc acctgggtgc
    71701 gaccaaacga cttcttaata ttggcaatct caatgctcat gatgttcctc ctgctgtgcg
    71761 cgtttatcct gattctccag gcgccactgc aacatacttt ttaaaaacag ggtgataatc
    71821 gccatcagcg ttaacagtgc cgcagcggta aaggagccga cggtgttgta gtcctgctcc
    71881 agcaattcaa tctgtaacgg cagcgacagg gtttcgccgc gaatcgagcc ggaaaccacc
    71941 gacaccgcgc caaactcgcc aattgcgcgg gcgttggtca acaccacgcc ataaagcagc
    72001 gcccagcgaa tgttcggcaa tgtgacgcgg cgaaacatct gccagccaga cgcgccaagc
    72061 aaaatcgccg cttcgtcttc ctggctgccc tggcttaaca tcaccggcac cagctcgcgc
    72121 accacaaacg gacacgtcac aaagatggtg accagcacca ttccgggcca ggagaacata
    72181 atttgcagat tatgttcatc cagccagccg cccagcgggc cgttagagcc gtagaacagc
    72241 agatacacca gaccggcaac caccggcgat acggcaaacg gaatgtccag tagcgtcagc
    72301 agtaactggc gtccagggaa gttaaagcgc gtcaccagcc aggccagcag aatgccgaac
    72361 accaggttta ccggtacggc aatcagcgcg atcatcaccg tcagccagat ggcgtgcagc
    72421 atgtccggat cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa tgcctgcacg
    72481 aagatgtaaa tcatcggcac cagcaggatg aacgctgaaa ccagcatccc gatgccaatt
    72541 agaaaccatt tgccccagtt aatcgggcgc gcgtcataac gcttcaattg ggtaacttcc
    72601 gccattaatg acctaccaca cgccgaccaa agcgactttg cagagtgtta attgagaaca
    72661 gcagcagcag ggatgccgcg aggatcaccg aagcaatcgc gctcgccgcc gggtaatcaa
    72721 actcctgcaa gcgcacaaaa atcatcagcg acgtcacttc cgtcttccag gcgatgttcc
    72781 cggcgataaa tatcaccgcg ccaaactcac caagactacg ggtaaacgac aacgccacgc
    72841 ccgccaccag cgctggagaa agttccggca gcaccacttt gcagaaactc tgccagcgcg
    72901 ttgcaccaag cgtttccgcc gcttcttcat attccgggcc taactcttcc agcaccggct
    72961 gcacggtacg caccacaaac ggaatgctgg taaaggccat cgccaccgca ataccaagcc
    73021 aggtataggt gactttgata tcaaacttcg ccagccattc accgtaaaaa ccgtttacgg
    73081 aaaagagcga ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag ggtaaatcca
    73141 tcagcgcatc aagcagcgtg cggcccggaa agcgataacg tgttaaaatc cacgccatca
    73201 gcagaccgaa aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc gtcactttgt
    73261 aggccgcgac cacttgtggg ttggtgatca cctcccagta ctgcgcccag ctcatctgag
    73321 ccagctgcat caccagcgcg gagagcggca gcagcaaaat caggcacaca aacagcagac
    73381 tggtgccgag gcttaaggta aagcccggca gcacgcgtct tgatgaaact gcaaacatca
    73441 gttacgcccc gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt gggttttcat
    73501 cacttccggc caggagccaa atttgtcttc cacgcggaac agctcggtct gcgggaattt
    73561 atctttcagt ttgtccatga cttccgggtt atttacgcgg tagtaatagt cggtgatgat
    73621 ggtctgcgcc tgcgggctgt agagccagtt caggtaggct tttgctgctt tttccgtacc
    73681 gttggcctgc acgtttttat cgacccacgc caccgggaat tccgccagaa tattggtttt
    73741 cgggatcacc acttcaaagc cctgtgcttc atactgttta cggatgttgt tcacttccga
    73801 ttcaaagcta atcagcacat cgcccaggcc acgctcggca aaagtggtgg ttgcgccacg
    73861 accgccggta tcgaacactt caacattttt caggaactgg gtcataaact gttcggtttt
    73921 ggctttatcg ccaccgtcag ctttatccgc tgcgccccat gccgccagat aggtataacg
    73981 cgcgttaccc gacgttttcg ggttcgggaa aatcagcttc acgtcggagc gcaccaggtc
    74041 gttccagtcg tggatattct tcgggttgcc cttacgcacc aggaagccca tggtggagta
    74101 gaacggcgag ctattattcg gcaggcgcga ctgccagtcc gccgggatca gcttgccttt
    74161 gtcatgcagg atttgtacgt cagtcacctg gttataagtg acaacgtcgg cttttaagcc
    74221 ctgtaaaatc gccagcgcct gttttgatga cccggcatga gattgtttaa tcgtcagttt
    74281 gtcgccgccg ttatctttgg cccattgctg ttcaaacggc gggttcaggg cggcaaacag
    74341 ctcgcgggag acgtcataag aactgttcag cagttccgtt gcctgtacat ggcctgccag
    74401 cagcagagaa gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg ccattgcgca
    74461 cccttatata tttaatgact ttctaatagc catcatattt ataacggacg tgaaaggagt
    74521 aacggtttta tataccgttt ggtgatttgg aagttgaaaa gggaataaga accgggttta
    74581 aaacgaaatc ctccccacac atgcagggag gaaacagagg tgaatcagat accgacgcta
    74641 accgtctccg gcagtgtgct gccgccatca atcacattct gtgtaccggt taaatagctg
    74701 gattcatccg atgcgaggaa ggccgccagt tcgccgactt ccagcggatc ggcgaggcga
    74761 cgcagcggga ttgcttttgc catatcagtc agcaccgact ctggatcttc cgggttcgac
    74821 tggcgggcaa tgctttccgc cattggcgtg cggacgtacc ccgggcagat ggcgttaacg
    74881 cgaataccgg attgcgcgta ctccaccgcc agcgattttg tcaggccaac aatcgccgct
    74941 ttcgttaagg cgtacgccgt ttcacccgga tcggccacca tatcaccagt gactgaagac
    75001 atcatcacaa tgcgaccatc tttgcgcgca atcatctctg gcagcaccgc cttcgtgacg
    75061 ttccatacgc ctttaatatt gatgtcaata tggaaatcgc gatcttcatc gctcatatcg
    75121 aggaaactgc ccagacgaca aacgcctgcg ttattcacca ggatatcaat gcgcccttct
    75181 ttttccttcg cgcgtttgat agctgcggct accgacgccg ggtcacgcac atcggcgaca
    75241 accgccgtac agcgatgacc acgaccacac agttcgtccg ccagcttttc gatctcaggg
    75301 gagatatcca gcaagattag gtttgcgcca tgacgcgcaa aagttctggc aattccttcg
    75361 ccaattccct gcaatgcgcc cgtaatcagt gctgtcttgc ccgtgagttt acccatttta
    75421 atgctctcct tgttaataca gagcgttaca ctccattccc tctgaaaata caggataaac
    75481 aaaatattgc tgcggatcag ctcactaatt caggaaaatc tcaatccagt cgctgcttta
    75541 tgcatcattt caggcgttgt gttagctcac tgctgcgctg aatcattttc aatgactcga
    75601 catcattgag ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc
    75661 gggtagacat cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat
    75721 gcgccagccg ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct
    75781 gctttcgtgc cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag
    75841 cgatttgtac atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg
    75901 cttcgcaggc cacgcgatat ccaattttca tcagtttgaa cgacagatcg cgccccacca
    75961 gcgccgatcc gcccaggccc gttatctgaa taaattgcgc tttactgatg acatcaatga
    76021 ttttttgcag ccgcgcgtag tcgaacaacg cgcaggtttg ctccagcgcc agctcttttt
    76081 cgcgattcag tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca
    76141 ggtgcagcgc cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca
    76201 tacgcaattc agtaaatcct tgtgcgccga gcttctgggc aaacttcaca atactcgact
    76261 ggctgatgcc gagctgtttc gccatcttgc gcgaagagac ggattgcagc tcactcacgt
    76321 tggcctgcag aaagtcagcg attttttgct cgttttcggt gaattcactc cccgcattgc
    76381 ttattttcgt caggtacaac atcttccact ctcctgggct ggcagcataa attccgctta
    76441 agttcttatg acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt
    76501 caattcggga tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac
    76561 caccgatgca actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg
    76621 accgcgtatc gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc
    76681 ttgccgttga gcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg
    76741 tcagcggcgg cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc
    76801 tggatgccag cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga
    76861 ttgctggagg cgaatatgcc attcagcacg cggtggaagg cgctgaagat agccgggaag
    76921 gtggtgttaa cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg
    76981 ctgccagcgg tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct
    77041 gccgcacagt gggaatttcc tgtaatccgg ggagtgccgt ttccaccacc gccgagtttg
    77101 ccattacacc gattgtaggt gccgaagtgg tcactggttc ttcacgaatg aaagcaggca
    77161 cagcgcagaa actggtgctc aatatgcttt ccaccgggct gatgattaaa tctggcaaag
    77221 tgttcggcaa cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg
    77281 tcaatatcgt taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg
    77341 cctgcgagcg caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag
    77401 aagctaaaaa acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat
    77461 aacccatggc caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg
    77521 gaccgggaaa tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg
    77581 acagttcact ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga
    77641 ccagcgacca ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga
    77701 tgagcgagct gctgggcgag gcaccggtac aggatgccgc agaaatcgcc gcccagaaca
    77761 aacgtcagtt aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca
    77821 ccatcttcac gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga
    77881 cgttaattgc cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc
    77941 tgaattttat gaaggtgttc agcaaaggtt tgttcacttt cctggtgatt ctggtgggct
    78001 ataacgccgc ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct
    78061 ttttgctcgg ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct
    78121 ttggtctgcc catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct
    78181 gcgcccgcat tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga
    78241 cctcgttaat caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg
    78301 gtggctggtt attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg
    78361 gttgtgcggt tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct
    78421 ttattcctgt ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc
    78481 tttcaatggc aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac
    78541 cgcacagtgc attacgcagt caggtacgcg gggcgattat tcccggtctg ctgggcgttg
    78601 gtgaaccgtt gatttacggc gtcaccctgc cccgcatgaa accgtttatt accgcctgtt
    78661 taggcggcgc agcgggcggt ttgtttatcg gcctgatagc ctggtggggt ctgccgatgg
    78721 gcttaaacag cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac
    78781 aaggcatcct tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg
    78841 ggtttatttt caccacgctc tttggctgcc gtaacgtcaa tctggactga tatcatgaaa
    78901 cggacaatgc tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac
    78961 cctgttctga cagaaagttc gccagagaaa gcagggttca acgtcgaacg gcttaaccag
    79021 atggatcgct ggattagcca gcaagtggac gccggttatc ccggcgtaaa cctgctgatc
    79081 attaaagata atcagattgt gtatcgcaaa gcctggggag cggcgaaaaa gtacgatggc
    79141 agcgtgttga tggaacagcc tgtcaaagcc accaccggga cgctgtatga cctggcctca
    79201 aacaccaaaa tgtacgccac gaacttcgcc ctgcaaaagc tgatgagcga aggcaaactg
    79261 catcctgacg atcggattgc gaaatatatt ccggggtttg ccgatagtcc aaatgacacc
    79321 atcaaaggca aaaacacgct gcggatttct gacctgctgc atcacagcgg cggtttcccg
    79381 gcggacccac aatatccgaa taaagcggtc gcgggcgcgt tatattccca ggataaaggc
    79441 cagacgctgg agatgatcaa gcgcacgccg ctggagtatc agcccggcag caagcatatt
    79501 tacagtgatg tcgattatat gctgcttgga tttatcgtcg agtcggtcac cggtcagccg
    79561 cttgaccgct atgttgaaga gtcgatttat cgcccgctcg gcctgacaca tacggtattt
    79621 aacccgctgc tgaaaggctt taaaccgcag caaattgccg ccacggaatt gaacggcaat
    79681 acccgcgatg gcgtgatcca tttcccgaat atccgcacct ccactctctg gggtcaggtg
    79741 cacgatgaaa aagcctttta ttcgatgggc ggcgtttccg ggcacgcagg tttgttttcc
    79801 aataccggcg atattgcggt gttaatgcaa acgatgctga acggcggggg ctatggcgat
    79861 gtgcagctgt tcagtgcgga aacggtgaag atgttcacca ccagctctaa ggaagatgcc
    79921 acttttggcc tcggctggcg cgtgaatggt aatgccacca tgacgccgac gtttggcacg
    79981 ctggcaagcc cgcaaaccta cggtcacact ggctggaccg gaacggtgac cgttatcgat
    80041 ccggtgaatc atatggcgat tgtgatgtta agcaacaagc cacattcgcc ggttgccgat
    80101 ccgcaaaaga atcccaatat gttcgaaagc ggtcagctgc cgattgcaac gtatggttgg
    80161 gtagttgatc aggtgtatgc ggcgttaaag caaaagtaat aaaaaggcca gtcagtaatg
    80221 ttactgcctg gcctttaatc aatgaatcag aaacgcttac agcgccatca acttgtccag
    80281 cgacggtgcg aaataatagc cgccggttac cggtttggtg aaacgcaaca tcgcatcacg
    80341 cttaccatcg gtatcgccaa acatgctcag cagttgctgc tcaatgttat gcagacgcgc
    80401 gcagtaggcg cagaagtaca gaccgtgagt gccactggca gtgccgtacg gcaggctctg
    80461 gcgaacaatc ttcagccctt tgccatcttc tttcagatca acgcgggtga ggtgagaggt
    80521 ttccggacgt tcgtcgccgt cgatctcttc gttggcctct ttggtgcgcc cgatcatcat
    80581 ctcctgatcg tgaacgctca tccggttgag ctgcttcagg ttgtgttccc aacgctggac
    80641 aaacacatag ctgccgcccg catccacgcc gtctttgata accgccactt cgcgacgcgt
    80701 ctcttcaccc gccgggtttt ccgtaccgtc aacaaagccg ctcaggtcac gctcttcaac
    80761 ccaacggaag ccgtggatct cttctttcac ttcaatgcag tcaccaaagg cttccatcgc
    80821 cgcctgggcg acagagaagt ttacgtcgtg acgcagagaa agaatgtgga tcaacacatc
    80881 gaactgggtg gtcggcgcaa ggcctttacc gtagcccgga aaatctttca gctcttctgc
    80941 cccaacgccg ccgctcagag cgcgccaggt gttgttacca aaggcaacca ccgcaccaag
    81001 atgcgcgtcc gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg ttttactggc
    81061 cgcacgcagg gcgtcaactt cccctttcac gttggcttcg atccaaatcg ccgcgcggca
    81121 atgttctggc aaaatgccac tctgaacctg agacattgtt cctcctgaaa ataataatgc
    81181 cacgcaatcc gtggcattgt tgagcgctat tttacgtgtt ttttcgcggc ggcggtttgt
    81241 tcagacgcaa attaacgccg ccagataatt ttactgactt tccagttttt cagggtatcg
    81301 tccgaaggca ttaacccttc aggaccactc cattccccgc tgaactggta gctaatatgt
    81361 tgactgcctt cggctttaca ttcgacggca cgattatcat caccatcagc tttttggcaa
    81421 ttgccaaatg ctttgctgta aaggtcgcta aacggtgtac cgattttaac accggtgtca
    81481 gcaggaatat cgctatccag cacgtcaatg cgactgatcg tgccttgatc gccgttaatc
    81541 accatcgcca cgttgtcgcc cttcatcact tcaaaaaagc gcaccacgtt gccgttcgcg
    81601 gttttcattc cgctgcgcag gcgataatcg ccatcaagcg catcggcaat ggcttgttct
    81661 tgcagtggtg tggacgccgt taattcacca acgccctgct cgctcacttt ggtggacgac
    81721 ccaaaccagt tccacgggtt agcggccgac cagttaactg acgacatcgt ggaacagccg
    81781 gtcagcatca acggcatagc gcataacatt aaacgcagcg atttcatgtc acttcctttg
    81841 gttattcaat aacgttgctt ggagtgcaaa ttcaccaaaa agtgccgtta ttctttttct
    81901 tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc cacaatatcc
    81961 gccactacca gcgccagacc gataccgcta acggattcac cgttcagcca cagccacggt
    82021 tgccagcaaa gtaaaaccac ctgcgccaac agcaacagaa aatagagcac atgccaagtg
    82081 cgaggaaatg tagcccgccg accgctcaac aaaaacgcca gcaccgccgg aatgcctgga
    82141 atcagcccca gccagaaatt atcgtgatcg ggataaaaca gatttagcag cgcagtaccc
    82201 tgctcgcgcg acgcaccggc aatgacaaac agcacccagg ttcgcgcctg aagcaatagc
    82261 acaagccaga agagcaaggg taaacgcagg cgtccgtgcg catcataatg gacaggatgg
    82321 aactcagtac tcttcatctt caatcaaacg cttacccaga ctcagcacgt cggcgtgttc
    82381 atatcccagg cgttcataca ttccgagcac catgtcgtta tcttccggca cattgatctg
    82441 aattttcggg cagccacgag caatcagctt tttctccagc cgattaagca acgcattggc
    82501 aatcccacgc ccacgaaact ctggatgcac gccaagataa taagcagacc cgcgatgccc
    82561 gtcataaccg cccatcaccg ttccgaccac ttcaccgttt acctcagcga ccaaaaacaa
    82621 actgacgtca tggttcatct tacgctcgat gtccatttcc ggatcgttcc acggacgcag
    82681 caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc gaaatacgcg
    82741 tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg cgtgaacggt
    82801 cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg gagttgtcaa
    82861 ctattgaaat gaaaagtaaa acaattctca acatcaaacc gtcgtaacgg attacgcgat
    82921 acgatataac atctggaact ttattattac aactcaggcc gtatgagcac ttttaaacca
    82981 ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagccg gtttggctgc cctgacgttg
    83041 tcaggaatgt cgcaagccat cgccaaagac gaacctttaa aaaccagcaa cggacacagc
    83101 aagccgaaag ccaaaaaatc tggcggcaaa cgtgtcgttg ttctcgatcc aggccacggc
    83161 ggaattgata ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt ggtgctggcg
    83221 attgctaaaa acgtccgttc cattttgcgt aatcatggga ttgatgcgcg tttaacgcgt
    83281 tctggcgata cgtttatccc actttacgat cgcgttgaaa tcgcccataa acatggcgca
    83341 gatctgttta tgtcaattca tgccgatggc tttaccaacc cgaaagctgc cggtgcttcg
    83401 gtatttgccc tctctaaccg tggggcaagt agcgcaatgg cgaaatacct gtctgaacgc
    83461 gaaaaccgcg ccgatgaagt tgccggtaaa aaggcgactg acaaggatca cctattgcag
    83521 caagtgctgt ttgatctggt gcaaaccgat actattaaaa acagcctgac gctcggctcg
    83581 catattctga aaaagattaa gccggtgcat aaactgcaca gccgcaacac cgaacaagcc
    83641 gcgtttgtgg tgttgaaatc gccgtcggtt ccttcggtgc tggtggaaac ctcgtttatc
    83701 accaacccgg aagaagagcg gctgttaggc acggcggcgt ttcgtcagaa aatcgccaca
    83761 gcgattgctg aaggcgtgat cagttatttc cactggttcg acaaccagaa agcacattcg
    83821 aaaaagcgat aagttatgaa acccgacgca caccaggtta aacagtttct gctcaacctt
    83881 caggatacga tttgtcagca gctgaccgcc gtcgatggcg cagaatttgt cgaagatagt
    83941 tggcagcgcg aagctggcgg cggcgggcgt agtcgggtgt tgcgtaatgg tggtgttttc
    84001 gaacaggcag gcgtcaactt ttctcacgtc cacggcgagg cgatgccagc ttccgccacc
    84061 gctcatcgcc cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc actggtagtg
    84121 catccgcata acccgtatgt tcccaccagc cacgcgaatg tgcgtttttt tattgccgaa
    84181 aaaccgggtg cagagcccgt ctggtggttt ggcggcggct tcgatttaac ccctttctat
    84241 ggttttgaag aagacgccat tcactggcac cgcaccgccc gtgacctgtg cctgccattt
    84301 ggtgaagacg tttatccccg ttacaaaaag tggtgcgacg attacttcta cctcaaacat
    84361 cgcaacgaac agcgcggtat tggcgggctg ttctttgatg atctgaacac gccagatttc
    84421 gaccactgtt ttgcctttat gcaggcgata ggcaaaggct acaccgacgc ttatttacca
    84481 attgtagagc gacgtaaagc gatggcctac ggcgagcgcg agcgcaattt tcagctctac
    84541 cgtcgcggtc gttatgtcga gttcaatctg gtctgggatc gcggcactct gtttggcctg
    84601 caaactggcg ggcgcaccga gtcgatcctg atgtcaatgc cgccactggt acgctgggag
    84661 tatgattatc agccaaaaga tggcagccca gaagcggcgt taagtgagtt tattaaggtc
    84721 agggattggg tgtaactccc tcacccccat tcccgcatcc gctgatgcaa cgtcaacgac
    84781 ggcttctcgg caaacagctg ctggtaatcc gtggcaaatt gccccagatg ccagaatccc
    84841 cactgcatgg cggcgtcttt taccgttgtg ctttgcgacc acgggcttat cagttcgcgg
    84901 cgtacggcgt tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc taaaatagcg
    84961 tgaaacgcgt tttgtagcgt gcggcgactg acatgcagtt gattacacaa gtccagcacc
    85021 gtcaccggct cagacatatt ttccagcaca tattcacggg cgcgggaaag taatctacgg
    85081 taactctgat gactgatgct ttccgccgtc accattggtt gcgcgtcttc cagcatcgcc
    85141 cccatcgcca ttagcaaatt atcccccagc acttttcgca ctgcaggctg atggagattt
    85201 tccgggttct cgcaaaacgt cgccagcgcc tgttggacaa agccccacag cgcggcttta
    85261 tgctgctctt tcacttccag cgccgactgg ctacgcagca tatgtaatac ccgatccggg
    85321 ttatgcaaaa agttagcctg ccgggtgatg acatcttcag aaagcaccac gcccaggatc
    85381 gtgtaatcat ccggcgtgct cagttcaaat tcagtgccac cagggcgggt ggcgatttcc
    85441 gcgcttccca gacattgcga accgataaat ccctgctcac cgcgcgtcgc cggaatgcca
    85501 aaccagaacg agttcggcca gaccaggcac gactgacgca gcgccagacc ggtgtattca
    85561 cgaaaaacct gaatatcatc gagtagaatt tccgtaaatt cacaatgaaa cttgcccgga
    85621 tgcagctgat cgtaaatctg ctgccaggcg gtaatcgtta aagcatgttc atagacatcc
    85681 gttgtctgtc gttgatgaac attatccact tcgaccttcg gcgtgagctt caggttttcg
    85741 ggtaagggtt catgataaag atggtgcaaa ttggctgtac gggtcttttt catgatgtta
    85801 atgccgggtg ttgtaggaca cccggcacct ccgacaggtt aatggggctt gagacgataa
    85861 cgactactgc gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc atttctggct
    85921 ttttccagcg gccagccaaa gtgggcagcc acttctcctg ccgtcattcc ctgacgtact
    85981 gatgttaaca gcgccaacaa ctcgtcagca gattccgcga cgataactgg cgcgtcgggt
    86041 tcccttacgg gttgcttcgg ctggcggtta aagccagtaa ccagccactg ggtgtcatcg
    86101 tctggccgac cgatctcctt gcggctgatg acacgcccgg tacgcatcgc ggcagcacaa
    86161 cctgcgtcca gcgccgcacg acacgccgcc agatcgcctt ccaccaccag cgttaagcga
    86221 ccagggtcaa gcacttcgtg actgagcaga cgaacgttag ctgctttgag catggcatcc
    86281 gctgcatcta tcgcggcgac catgccgtcc acttccagca atcccagtgc attgatcatc
    86341 ggcaacctcc gttacgcacg ctggattgga ttacgcgcga tttccagcac tgcatcggta
    86401 aaggcgttac aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc tgccgagtag
    86461 ttggtttcag acggtggcgg gacataggtc accagctgta cgtcggcaga tttcaacgct
    86521 gcatcaatgc cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca
    86581 agcgtgatcc cggcggttga tgagagataa gaaccggtac gcgaaactac atgtgccagg
    86641 aaggcggtgt tttctgcgtc attggcccac tggaaagctg cgccattttc aatatgtgca
    86701 accatcgcat ccagaccggc acgcacttcc gccgggtttg gcccaccaag cataatcagc
    86761 acttcaccgg cagtcggtga cgggccgtgt gccgctcccg catacagcga gcgaccatat
    86821 accacttcga ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt gacgtcgtca
    86881 gaatcggcag aaatcagccc gaggctacga atatgcggcg gcaattttag ttcacgcgca
    86941 aattcagcgt taacggaggc aatcacccgc atggcggtta ccgacggtcg aatcaaatct
    87001 aaagctggca tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc
    87061 atacgtttcg ccaaatccac aatgacggct gcggcttcaa ccggcggcgt gccgccctgg
    87121 tgaatgttag agatacaggt gcgatcggcc tcaacggtgg tcgccatacg cggcgagtaa
    87181 acggcgtagc aggagaggct ttctgactgc cctaaccccg gacgttcccc caccagcagg
    87241 atcaccactt tcgcgccgag gatctcaccg atctgatctt caatcttcac gcgaccataa
    87301 cgaacaaaga atggcgtgcc gactttcagc ccggcctgtt tcaggcccgc catcagcggc
    87361 ggcaggatct cttcgtagtt cacggtgatg gcatcagttg acaggccatc agaaataacg
    87421 acctgtacgt ccggattagc aacacactgt gctttcagcg cttcaacagc ttctgcacac
    87481 aggcggcggc ccatatccgg gcgagtcagg tagagatttt tgtcgctaat ttcggagcgt
    87541 acttccagca agccctgcgc tttcacccac tcttccggca cttctttcag tacggtatct
    87601 ttcgaacgag agtgatcggc caggaaacgc agcaacgcct gcgtacgcgg acgcggaccg
    87661 gcacgaccgg tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa tacgtctgcg
    87721 cgatgcgggt tttcaacacc aatccacgct tttgcttccg cagaacctaa atccagcgcg
    87781 cagctttccg aggtcaccgg tgccgcacag ttggtggtgg cgcactttgc ttctgacggc
    87841 gcgggggccg tttgtcccat tgacgccatc acgctgcgta caatttcttc aatctgtttt
    87901 tgatccatga tgtgttatct ccgcgtcatc agaagaacag tgacggatcg cccgcccgtt
    87961 tggtcaggcg accgtttgcc ataatgccca tgctttccag ccagcgttca aactccggtg
    88021 acggacgcag gttgagtaac tgacgcacag tggcggtatc gtggaatgcg gtggtctggt
    88081 agttgagcat gatgtcatca cccagcggca tccccatgat gtagttgcag cctgcggtgg
    88141 cgagcaggat catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg ttggtgtaac
    88201 agcagtcaca gcccatagag atgccgctca gcttgcccat aaagtgatct tctaagccag
    88261 cacggataat ctggcggtcg ttgtagagat actccggccc aataaagccg accacggtgt
    88321 tgacgataaa cggatcgtaa tgacgcgcca gcccatagtt acgtgcttcc atcgtcacct
    88381 ggtcagcgcc gaagttagcg ccagcggata gcgcagagcc ttgtccggtt tcgaagtaga
    88441 ggcagttttc cccggcgata cggttgaact ctgcacccac tgcgcgcgct tcgtcgagca
    88501 tcgccagttc cacgccaaac tctttcagcc ctttttcgct gccacagata ctctggaaaa
    88561 tcagtccgcc aggcgcgccg cgacgaatcg cttcgatctg ggtggtgacg tgcgccagta
    88621 cgcagccctg agttgggatg ttgaatttgt cgatcacgcc gtaaatggtg tccaacacgc
    88681 ggcttaagtt ttccacgtcg tcagttaccg ggttaacgcc gatcaccgca tcgcccaccc
    88741 caaaggaaag cccttcatag atttgcgcag cgatactttg cacgtcgtca cgggtatcgt
    88801 tcggctgcaa acgggcgctg aaggtgcccg gaataccaat ggtggtattg gcctttttga
    88861 ttaccggcat tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc ttcgctaccg
    88921 ccgcgaccac ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc acgctggttt
    88981 catcgctcag cacatactca cgcagttcgc tgatgctcca gtttttaatc tggttgtagg
    89041 ccgtttcgtt aacatcgtcc tgaatcagcc gcgtcacgca gtcatcttca taggcaatca
    89101 ccggattatt gcggatgtcc gctacggtca tttccgacaa cacctgcttt gccgccacgc
    89161 gctcctgtga gcttgccgct gcaacgcccg ccagcacatc ccccgaacgc agttcgttgg
    89221 ctttagccag cacctctttt acatccttaa actgatatac attgccgaac aatgtggtct
    89281 ttagtttcat aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg tcaccggcac
    89341 aaccgatccg ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac
    89401 ttcgtcaatg actgccaacg ggagttgttg tagctgtggg cgcaacaaca tgcccagagc
    89461 tttaccaaag tcctgcccgg ccaccaccag caggggatgc ggattcggaa aacgcgcgac
    89521 gaaatcgacc agcgcgttga tgaccgtcag taccgcggcg taacgcacag gcagcgaggc
    89581 gggaagcgcc agcacgtacg cgtcagtttt gggatcaaga tccagctgaa gcagcgcctg
    89641 ttgccaggca ctcaccagat ccgtttcatc aatcgggatc gccaccggca aattgcgcag
    89701 cggcagttgt acgccctcca gccagattgt gctgccagag agcgaaaggg tatgtgcacc
    89761 cgcgccaatc accgtggcgc gtacggtttg cgccggaaac tgcacattca tctcacgcag
    89821 gcgcggatgg tcatgcagcg ccgttgccag aagtgggcca atatcggcaa aacagaacgg
    89881 gtcggcgggc tggtggcgat aacattcgcc cacgcctcca gaaagcgtaa tgatttcggg
    89941 cgtaacacct gcgggcagca aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt
    90001 tccgtcaatc acttcgacaa tcagcgccgc catccgccgg gtaacctgca ccagctgcgc
    90061 gccggtcagc gaacgggcgt cagtgcctgc accgaagcac tcatccacaa tcatctgccc
    90121 cggtttatga gcgtaaacca cgcgcccctg gctgtcggtt tccatcaggc gaccaccgac
    90181 gttgagacag gcagtgccgc tgatttttcc ggcatcgaac agggcgtagt tcgcggtgcc
    90241 accgccgatg tcgatattca gtacccgaca cagccgttgt tcagaaaggg tttgcgcccc
    90301 agctccgtga ccggcgatca cggattcgag gtgcggcccg gcgctggcaa cgacaaaatc
    90361 ccccagcgat tgagagagcg ccatcaccgc cgggcgagca ttgcgggttt tcgcgctttc
    90421 accggtgatg atgatggcac cagaatcaac gctttccggc gcaatacccg cagcctgata
    90481 ttgctcgagt attaaggttt tcagttccgc ttcttttaaa ccgccctgtt tatcgacagg
    90541 ggtaaagaac accggacttt gccagctaat ttcgcgttta atgaattcgt agcgcggcac
    90601 ctgcgacacc gccgcacggt taaccagctc caggtgggag aaaatcacct gggtggtggt
    90661 ggtgccgata tcgataccga cgctcaatag ctggcgagtg ttcacgattg tgcctccgct
    90721 tcggttttag tcgcggtcgc gtcttctttt ggcaccagca tcatcgccac gccaatcgcc
    90781 gttacgccgc cgatcaactt gccgacaatc atcgggaaga tcatggcgtt catgttggca
    90841 gcggcgaagc ctaagtggtc gcccagggcg aaagcagcgg aaacggcgaa ggcgcagttg
    90901 atgactttgc cgcgggtatc catctgcttc atcatgccga acatcgggat gttgttggca
    90961 agcgttgcca ccatgccggc tgccgcgatg ttgttcatat tcagcacttt accgacgctc
    91021 atcagcggtt tttcaaacca gcgagtcagc agcagcacca ttggatacgc ccctaacagc
    91081 acgcaggaga tagaaccgat aacttcaatg gcgcgcatca cttcaccggg tttatcgcca
    91141 ggggccataa agataggatc cagtccgggg atcagttccc agccaaggag gaatttcact
    91201 accgcagcgg caagaccgag ggtgatcaat gccacgagga atttggcgaa gatctggaag
    91261 ccgttgatca ttttttccgg gatgaatttc agccccagcg ccaccagaat cgcgacaatg
    91321 atcaccggga tcatgttcat caggatcagg gcgaaagtga attccaccgg ctgaccgttg
    91381 atctgcacac cggagtacat agcaaccaga ccaccagcaa tacagccaat cggaatggtc
    91441 acaatgcccg ccagcacgcc gagcgccaga taacgacggt cagaaggttc gataatgccg
    91501 agcgccaccg gaatggaaaa cacaatcgtt ggccccatca tcgacccgag aattaaccca
    91561 gagtatagcc acgctgctac gtcgccgccc gccagctctt tggcgaggaa gaagccgccc
    91621 atatcgcacg ccagcagtgt tccggcgaac atcgatgggt tagcgccgag catttcgtaa
    91681 accggaataa ttaccggccc gagtacgtga gccagtaccg gtgccagcgc ggtcataccg
    91741 accatcgcca ggcccagtgc gcccattgcc ataaagcctt cttcgaactg accgcctgat
    91801 ccttcgatac ttttaccgaa cttaccgagg aaacgagcag aaccgccgaa ctgcgacagg
    91861 atcctgtcta cggcagctat cagcataaag aacatcatga tgtacatgat gatttcgtta
    91921 attcccatcg cctttactcc ctgttagttg ttatttattg gcggatgcgg cgtaaacgcc
    91981 ttatccgccc tacatgtgca atcccgaagg ccggatcaga cgcggcaagc gtcgcatccg
    92041 gcatttattg cgccgctgcg tacaggccga caatctgctc caggctggcg gtacgcgggt
    92101 tgctgcgcag acaaatatct tcctgcgcgg cctgcgccca tgcaccgtaa tgcgcagatg
    92161 tcgcgccaac atcgcccagt cgtttaccaa tcccaacttc cgcaatcagc tcacttaccg
    92221 cgttaatagc gtcacgatcg tcggattttt tagttcgcag tgcccgacca atctgactaa
    92281 accgttcgcg acaaaccatc cggttaaatt ccatcaccgt tggcagcaac atggcgttcg
    92341 ccagaccgtg tggaatatgc agcgccgccc ctggctgatg cgccatcgcg tggcacaacc
    92401 caagccccgc actggaaaac gccattcccg ccatacatga agccagtaac atgctctcgc
    92461 gcgcggcaag gtcatgaccg tagcccaccg ctttcggcag cgatttgcca atcatcgcaa
    92521 tggcaccaat cgccagactg tcggtaaacg gtgtagcgtt cagggcgctg tatgcttcaa
    92581 tggcatgggt taacgcatca atgccggtca tcgccgtgac atgcgacggc acaccttcgg
    92641 tcaatgcggc gtcgagaatc gccacatccg gcatcagcga ggcatgggct aacacctgct
    92701 tacgcccgct caccgcatcg ataatcaccg ttacgttggt ggtttcagag ccggttccgg
    92761 cggtagtggg aatggcaatc aacggcaggc gcgattgcag aacgctggtt tctgacatct
    92821 ctgccagcgt gctatccggg ttcgtcacca gcaacgccac ggctttcgcc gcatccagca
    92881 ccgagccgcc gccaaatgcg atcaccccat cacagcctga ctcacgcaac tgcgccacgg
    92941 ctgcacacac gtcggtgatg cacggttcgc ccaccggaca tggccagagc gtcatggcga
    93001 tacctttaac ggccaggcta cgcgtcagcc cggcggtcat ccctgcctga tgcaaaaagc
    93061 tgtctgccat cacgaacaga tgtttcagcc cacgcgtttg cgcttgctgt ccgcaactgc
    93121 tcaccgagcc cggaccgcaa agcgtcaccg gtggaacgct aaatgttttt acccgttgca
    93181 gattcagggt atcgaacgcc tgaaagagcg cggtctgcaa ttcattttgc atatagcccc
    93241 tccgctttct ctcgcccact actggcgatc gccagcgggg tcataaacaa actgtgctgc
    93301 ggtaaatgca cctgtaacgc cgggaattgt ttgcgaaaca gttccgccac gcccggttgc
    93361 atacaagagc cgcccgccag ccataaatca gtaatgccct gcccttcaat atgccgggcg
    93421 acaatgtccg ccattttttc gtacaccggt ttcaccgcgg gccaaatctc gtcaccatga
    93481 ccgcgcttgt actgctctgc ctcttccagc gaaatacgac gatttccggc gagagtcaga
    93541 gagatgtgat gtccgccggt ggcttcatcc gccgagtacg tcaccttgcc ctttttcacg
    93601 atggcaatgc cggtagtgcc gccgccgata tccactacac cggcgttgtc cagttgcagc
    93661 agatccgcga ctgcggttgg ctcatccagc acatggctca cttccagccc ggcagattcc
    93721 agcacgttga tggaaatacg cgggtccgta cctggaggaa atgaagtcgc cgcatggcta
    93781 aaacgaagac cgaattgctg ctcaagagtg tcgagatggc gacgaacaat ggtgaccgca
    93841 ccgaagaaat cccagacgat gccgtcgcgg accacatcgg cccagtcgag gcataccgcc
    93901 accggctgac cgtcgcggtc aacaaccatc gacaccacat cgcaggtgcc cagatccacc
    93961 cccagccaca gcggagattc cgtcgcggca ggcgtctggt tacacagcgt tgccgccgtt
    94021 tgcagacgtg gggtgagcca ttgttcgtcg tgcgccatcg gttactcctt aaacaatgcg
    94081 aaacgcatcg actaatacac agcgacgcag acggacaaac gtccgcgcgc tggttacccc
    94141 ttcaccggtt ggcgtggtga tggtcatggt ggtccagcct tccccgccca gccccagccc
    94201 ggcaatgcac ggtccgttct taacgaaaat gctggtatca atggcgttcg ccatctggtt
    94261 catgttttcg atattgcgcg agtgcattgc cgccgtgtgg tggcaaccgc cctccagttt
    94321 caccgccagc gcaatggcat ccgccacgtt ggcgacgcgc acgacgggca gcaccggcat
    94381 catcagttcg gtcacggcaa acggatgttc tgcagtggtt tccacaaaca gcaaacgcgt
    94441 ttcttgcggc acgttcaggc cgattgccgc cgcgattttc gccgcatcgc gaccaaccca
    94501 gtcacggctg acggtgcctt ttccgcgctc gtcgatattt ttcagcaaca ccggttgcag
    94561 ctgctgcgcc tgttctgcgg tcagtttcac cgcgtgctgg ccttccatca gacgcatcag
    94621 ttcatcggct acgctatcaa caacaatcag taccttttcg tcggcacaaa tgatgttgtt
    94681 atcgaaagaa gcgcctttga cgatggactg agcggcacgg gcgaggtcgg cggtttcatc
    94741 caccactacc ggcgggttgc cagcgcctgc ggcaatcaga cgtttattgg tgtgtttacg
    94801 cgccgcttct actaccgctt cgccgccggt taccaccagc aggccgatac ccggaaactt
    94861 gaacaagcgt tgcgcggttt cgatatccgg atttgccaca gtaaccagta agttttccgg
    94921 cccacctgcg gcaacaatcg cctggttgag cagcgtaatc gcccgctggg agactttttt
    94981 cgccgccgga tgcggggcaa aaatgacgct gttgcccgcg caatcaggct gatggcgttg
    95041 ttaattacgg ttgccgccgg gttagtggaa ggcgtcaccg aagccaccac gccccagggt
    95101 gcgttttcaa ttagggtcag gccgttgtcg ccagtcagca cttgcggaga gaggcactca
    95161 acgcctggtg tgccgcgcgc ctgagcgacg ttttttgcaa atttatcttc aacgcgcccc
    95221 atgccggttt cactgacggc aagttccgct aaatctctgg cgtgtttttc gcctgcttca
    95281 cgaatggcag caatggctaa ctggcgcatt gccacgcttt ttaacccttg ctgggcgact
    95341 ttggctgccg caacggcgtc atccagggac gcgaaaacgc ccatctcatg aacggcggcg
    95401 gacggcgtgt cactgctttg cattttcagc agtaccgctt tcaccacctg ttcaatatcc
    95461 tgttgattca tgatgttctg ccttatttgt ggaaaattac ctgaccgcca gacaccacct
    95521 catcgacaat gccaatcacg cacagatcga ccggtgacgt ttcgctttta tgcgcctggc
    95581 gggcggaact gccactcacc agtaacaccc actccccggt tcccgcgcca atattgtcga
    95641 tggcgacggc gcattgcccg tcaggattac cttgtggatc aatcatttcc accatcagca
    95701 atttgtcatg cgccagtccg tgatggcgta cggtacaaac aatttgtcca gtgacgactg
    95761 ccagtttcat acccgcctcc gtggcgtatt tcaggtaaaa gctcccccta ccctccgcag
    95821 aaggtaaaat gaaaaaggag agagcgtgac gcccgaatcg acgtcacaca gggtgattac
    95881 aggttgctgc tatcgccttt caggccgatc gggaagactt cttccaggtc gccgtgcgga
    95941 cgcgggataa cgtgtacaga taccagctcg ccgatacgct gtgccgctgc ggcacctgca
    96001 tcggtcgcgg ctttacaggc tgcaacatca ccacgaacca tggcagtaca caggccgccg
    96061 ccaatctgct taacaccaac cagcttgacg cgcgcagctt tcaccatcgc gtcagaagcc
    96121 tcaatcagtg caaccaggcc ccgggtttcg atcattccta atgcttccat tgtgctttcc
    96181 tctttatcag ggtccagaac gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc
    96241 ggttcacttc tgtctgacgc ggcacagctg ccaccagcgc cagctcgata atttcctgca
    96301 cgctacaacc acgagagaga tcgtgcatcg gcgcggcaag tccttgtatc agtggcccga
    96361 cggcacgata tccgccgagt cgttgtgcga ttttgtaacc aatatttccg gcttccagcg
    96421 acggaaaaac catcacattg gccttgccct gtagcgggct ggcaggcgct ttttgcgccg
    96481 ccacttccgg cacgaaggcg gcgtcaaact gtaactcgcc atccaccacc agctttggtg
    96541 cgcgctcacg gacgatttct gtcgcctgct ggacgttagc aacacagggg tgacgggcgc
    96601 taccattgct ggaaaacgac agcatcgcca cgcgcggctc ttctccggtg atggcgcgcc
    96661 aggtttcggc actggcaagc gcgatatccg ccagctgcgc cgccgtcggc tgtggcacca
    96721 cgctgcaatc ggcaaagccc aacgccgggc cgctgtactg tggcaacatc aggaaaatgg
    96781 aagagagcgt tttacagcct ggctgcaagc cgataatgcg taatccggca cgcagcacat
    96841 ttgccgtgga agagaggttg cccgcgatac agacatccgc tttaccggcg ctgaccattg
    96901 ctgcggcgaa catcagaggg tcggtaagtt tttccagcgc atccggcggc gttttttcgc
    96961 ccgcgcgggc cagccagcga tgagcaaatt cttcccgcat tgcgaggttg ccatgcggat
    97021 caatcacctg tagcccgtcc atcgccacgc cgtgactgag cgcaaactga cgaagttcaa
    97081 acggattggc gaccagaatg ggcgttgcca gaccttgttg atgtaaatat tgcgcggctt
    97141 tcagcacacg ttggtctaac gcatccggaa aaaccactct ggcgggcgct cgcaacgcca
    97201 gttcacgaca acgttcaata atcattgcgt ttcccccagt cgttgttgga tctgcttcac
    97261 cgttagcggc tgcttcgtta cgctcaggat catcatcacg tagaccgtgc tggaaaggcg
    97321 gttcagcgcc tgcaaaatgt ccgggcgtaa tacttcaaag ctgcgggtga taaacacctg
    97381 cgccgctacg gtttcggttt cccgcacttt ggtacgtagc agattgagca acgccgcatc
    97441 gcgcccgtgg ctggcttccg gcaccagatg atcgtggtcg agatagcgca gcggctggtg
    97501 agaaagtcga tgcagatctt cgtcagaaag cccgacaatc gcctggcagc ccagcggttc
    97561 acccagcgca tcggcgcgca taatgttgcc aagacgcgaa cggatatccg ccagccacgg
    97621 ctgccacggt tccgccagtt caatttgcag ccacactgcc agcgcaatgg tgctgtcgag
    97681 aaccgcgcga aaacccagac gcggatcgct tttggcgacc attttttccg ccgacaggtg
    97741 agtcagcgta tccggctttt ttgccaccgg ctggcggcac agttcgcagc acgcctgcgg
    97801 atgttcatcg ctactggtca gcccatgaac gggctgcggc tgctgctgtt catcgtcaac
    97861 aaacaggcgg ccctgctcgt caataaactt gatgcgcaga tggcggcttt ccagtaactc
    97921 ccgggcagag ggcgtcaggc gactgtccgc agggagatgg atctctgcgc cttcgctgag
    97981 cgtatggttc gctcttagcc atgcttcggt gatgaaatct ttcataggga ttgccagtta
    98041 gccggccagg cgacatacag gaatttcacg ctggatgtcg taccaaattc gatactggag
    98101 cctttcggga taaacatcac gtcacccgct ttggcgatca tggtttcgcc ttcgtggcga
    98161 acatgcagct ccccttccag caccatgtcg atttcgtcgt agttcagggt ccacgggaag
    98221 aatgcgttct cccactgcat aaaaccagcg gccatgctgc tgccgtcgtc tccggtcaca
    98281 agatcagtca agcccacgca atgcggctct gcgccgtcga aacgaccaaa cttgacgctg
    98341 ctgccatcaa tcactttgat gccgcctttg ccggtcaccg atttgaagct cggctgcatt
    98401 gcgccctgct ccagcgactg tttttccttc atcacttttt ccatcagctg tgcgaccagg
    98461 ctttcggtaa actggccttc cggcagctgg gcaatgatgg tttcgcggat gcgctggctt
    98521 tcggttttgt cggctggcac gctggcgggc acggacgccg ttaccgggat cgattcatca
    98581 cattcggtga tggtaaaacc cagtagatcc gccacttcac gggcttccgg ggtaataatg
    98641 ctggcgcgca gaaccacgga cattgcctgt tcgccgcgcg cgtgtgcttc acgaatatca
    98701 ttcgctgtga tgagtttttt cacctgcttc ctcctgtttg gtgagagatg ccagataatc
    98761 caccagttgc tgcacgcttt gcgggtcgtg gctgtttagc tcgaaaatcg gctcttcgaa
    98821 acccgtctca agcaataact ttcgcgttgc ggcgacgtca gcatctggca tgtccgtttt
    98881 actgatgacg gcgatttgcc gtttgctgac gccaatatcc agcaacccgg caggcaaccg
    98941 actttccggg tcatttgcgc catggacata gatcagcatg tctacatcct gtagcgtggt
    99001 aattaaggcg tgataccagc ggggatggct aaaatattca cccggcgtat caatatcgcc
    99061 cttatcatta aattccacgg cctgtgtttt tctggcgagg gtataattcc cctgcaacgc
    99121 attaaataac gttgtttttc ctgccccgac ggtacccaca aacgcaattc gtttcatgaa
    99181 tgcctcgtat taacttttgg tcatttcaca aagcgtataa tttaataagc gacctaaacc
    99241 gctgacggtt tgtgataacg cttcttctac tgcgcccacc gagccataaa tcaccaatgc
    99301 accgctgaag cggtcgagaa aaccgatatg cacatcagcg gctttcagcg ctaaatcacc
    99361 ggcgatcatc gccgtttcgc cgggagttag cgtcataatg ccaattgcgc ccgcatccgg
    99421 aacgccgatt ttcttcgcca gttcctcacc gggatgcgcg atgagatgcg ccagtgtgac
    99481 ctgtttgccg ggcacaaatt cctgaatgat gcgttcttta tccatgagtc gccgcctgtg
    99541 aaaaactttc gtgtatcgtg acaatttttc gtcagagtaa ataacaaaat tcggcaagcg
    99601 cgtttaaaag gtgaggtaga tcactaagaa tggagagata ttcgctgtgg tgcataaact
    99661 ttcatatgca acgcatgaat ataaataaaa taatatcgag atattatata gactgctttt
    99721 tttattattc gcaaactcag taataagctt tactaatata ttgcagccaa accgcttacc
    99781 ctgaatattc agggtaagcg tgagagttaa aaaagaatta cagcggttgg gtttgcgctt
    99841 caaccacggc cagcgccacc atgttgacga tacgacgcac cgatgcgatc ggcgttaaca
    99901 cgtgaaccgg tttcgcaacg cccatcagca ccggaccgac agtcacacct tccgagctgg
    99961 aaacgcgcag taagttgtaa ctaatgcggg cagcttccat gttcggcatc accagaatat
   100021 tggcagaacc tttcaaaggg ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg
   100081 ctgcatcgcc gtgcatttca ccatcaatca tcagatctgg cgcacgatcc ctgaccagtt
   100141 ccagcgcctg acgcattttg ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg
   100201 acaacagagc aacgcgcggc tcaataccaa aacgacggac agtttctgcc gccatcaagg
   100261 tgatctccgc cagctcttct gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg
   100321 tgttaccact cggcagcagc agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat
   100381 aaccaaagac atttttcacc acgctaaaat gttcatgata atcgcccacc gtaccgcaga
   100441 tcattgcatc ggcttcccca cgctgaacca tgatcgcgcc gatcactgtc gggttactga
   100501 tcagcgcccg ctgcgcctgt tcctgagtga caccgcgacg cttcatgatc tggaagtatt
   100561 cggtccagta ctctttaaag cgcggatcgg attcgttatt gacgatctca aaatcaacgc
   100621 ccgctttgat ctgcaagccc agtttctgaa tgcgcatttc gatcacgttc ggacgaccga
   100681 taaggatcgg tttcgccagt cccagcgtta ccagttcctg agtggcatgc agaacgcgcg
   100741 cctcttcccc ttctgacaga acaacgcgct tcggcgcttt gcgagcctgg gagaaaatcg
   100801 gcttcataaa caggttggtt ttgtaaacga actcagtcag cttgtcgatg tagacgtcga
   100861 aatcagcaat cggacgcgtc gccacgcccg actccatcgc ggctttagcg accgcaggag
   100921 cgatcttaac gatcaagcgc ggatcaaacg gttttggaat gatgtattcc ggaccaaagc
   100981 tcagatcctg atcgccatac gctgaagcca ccacttcgct ctgttcggcg tgggccagtt
   101041 ccgcaatcgc gcgtaccgcc gccagtttca tctcttcatt gatagccgtt gcgccaacat
   101101 ccagcgcgcc acggaagata aacgggaagc aaaggacgtt gttgacctgg tttggatagt
   101161 cagaacgacc ggtgcaaatg atggcatccg gacgcacttc tttcgccagc ggcggcagaa
   101221 tttccggttc cgggttcgcc agcgccagga tcattggcgc acgagccatt ttcttcacca
   101281 tttcctgggt cagcactttc gggccggaac atccaaggaa aatatccgcg ccttcaatca
   101341 catcatcgag ggtacgtttg ccgtcatcca ccaccgcata tgcggctttg gtttccgcca
   101401 tgtttggctc acgcccctga tagataacgc cttttgaatc gcaaaccacg atgttatgtt
   101461 tttgcagacc cagcgctacc agcaggttca tacaggcgat tgctgcggca cccgcgccgg
   101521 aaaccaccat ccgcacgtcg gagatgtttt tctccaccac gcgcaagccg ttgaggatgg
   101581 cggcagtgct gatgattgcc gtgccgtgct gatcgtcgtg gaataccgga atattcatcc
   101641 gctcgcgcag tttctgttca atatagaaac attctggcgc tttaatgtct tcgaggttga
   101701 ttccgccgaa ggtcggttcg agcgcagcga caacttcaat aaatttgtcc gggtcgagtt
   101761 catcaacttc aatgtcaaat acatcaatcc cggcgaattt cttaaacaga acgcccttgc
   101821 cttccatcac cggtttgcca gccagcgcgc caatgttgcc taaccccagc accgccgtac
   101881 cgttagagat caccgccacc aggttaccgc gcgcggtata tttgtaggct tttaacgggt
   101941 ctttttcgat ttcaagacaa ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc
   102001 gctgcgttgc cagaggcttg gttggagaaa cctggatttt ccctggaact ggaaattcat
   102061 ggaaatcaag tgcactttgt tttaactggt catccatttg gttgttcctt tcacgtaacg
   102121 ttcacaaata aagtgtgtgg gcaacagccc ctgcccacaa cgtggcgcac attattaccc
   102181 tgccggagtc tacagacttt gagcaagtcc aaactctcac cattaatata atgttttggt
   102241 aataatccta taacactgat gttacctgct taatccagca ataccatgcc tgtctgctat
   102301 gcttttttga tgcgtttagc gaaatttctc agaagtgtga attaacgcac tcatctaaca
   102361 ctttactttt caaggagtat ttcctatgaa cgagttagac ggcatcaaac agttcaccac
   102421 tgtcgtggca gacagcggcg atattgagtc cattcgccat tatcatcccc aggatgccac
   102481 caccaatcct tcgctgttac tcaaagctgc cggattatca caatatgagc atttaataga
   102541 cgatgctatc gcctggggta aaaaaaatgg caagacccag gaacaacagg tggtcgcagc
   102601 gtgtgacaaa ctggcagtca atttcggtgc tgaaattctc aaaatcgtac ccggtcgcgt
   102661 gtcaacagaa gttgatgcac gcctctcttt tgataaagaa aagagtattg aaaaggcgcg
   102721 ccatctggtg gacttgtatc agcaacaagg cgttgagaaa tcacgcattt tgatcaaact
   102781 ggcttcgacc tgggaaggaa ttcgcgcggc cgaagagctg gaaaaagaag gtattaactg
   102841 caacctgacg ctgctgtttt cttttgccca ggcgcgggcc tgtgcagaag cgggcgtttt
   102901 tctgatttcg ccgtttgtcg ggcgtattta tgactggtat caggcacgca agccgatgga
   102961 cccgtatgtg gtggaagaag atccgggcgt taaatcggtg cgcaatatct acgactacta
   103021 taagcaacac cactatgaaa ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat
   103081 cctcgcctta accggctgcg atcgactgac tatcgcaccg aatttgctga aggagctgca
   103141 ggaaaaagtt tcgccagtgg tacgtaaatt aatcccgcct tctcagacgt tcccacgccc
   103201 agcacccatg agcgaagcgg agttccgttg ggagcacaat caggatgcga tggcggtaga
   103261 aaaactgtct gaaggcattc gtctgttcgc tgttgatcaa cgcaaactgg aagatcttct
   103321 tgccgccaaa ctataaacca gccacggagt gttatatgtc ccgaaaagac cttgccaatg
   103381 cgattcgcgc actcagtatg gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc
   103441 cgatgggcat ggctgatatt gccgaagtgc tgtggaacga ttttcttaaa cataatccta
   103501 ccgacccaac ctggtatgat cgcgaccgct ttattctttc caacggtcac gcgtcgatgc
   103561 tgctctacag tttgctacat ctgaccggtt acgacctgcc gctggaagaa ctgaagaact
   103621 tccgtcagtt gcattcgaaa accccaggcc acccggagat tggctatacg ccaggcgttg
   103681 aaaccaccac cggcccgctt ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag
   103741 agcgtacact ggcggcgcag tttaaccagc cagaccatga gatcgtcgat cacttcacct
   103801 atgtgtttat gggcgacggc tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg
   103861 caggcacgct gggactgggc aagctgattg gtttttacga tcacaacggt atttccatcg
   103921 acggtgaaac agaaggctgg tttaccgacg atacggcaaa acgttttgaa gcctatcact
   103981 ggcatgtgat ccatgaaatc gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg
   104041 aagcgcaaag catgaaagat aagccgtcgc tgattatctg ccgtacggtg attggctttg
   104101 gttcgccgaa taaagcaggt aaggaagagg cgcacggcgc accactgggg gaagaagaag
   104161 tggcgctggc acggcaaaaa ctgggctggc accatccgcc atttgagatc cctaaaaaga
   104221 tttatcacgc ctgggatgcc cgcgaaaaag gcgaaaaagc gcagcagagc tggaatgaga
   104281 agtttgccgc ctataaaaag gctcatccgc aactggcaga agagtttacc cgtcggatga
   104341 gcggtggttt accgaaggac tgggagaaaa cgactcagaa atatatcaat gagttgcagg
   104401 cgaatccggc gaaaatcgcc acccgtaagg cttcgcaaaa tacgcttaac gcttacggac
   104461 cgatgctacc ggagctgctc ggaggttcag cggatctggc tcccagcaac ctcaccatct
   104521 ggaaaggctc tgtgtcgctg aaggaagatc cggcgggcaa ctacattcac tacggggtgc
   104581 gtgaatttgg catgaccgct atcgccaacg gcattgcgca ccacggcggc tttgtgccgt
   104641 ataccgcgac gttcctgatg tttgttgaat acgcccgtaa tgccgcgcgg atggcggcac
   104701 tgatgaaagc acggcagatt atggtttata cccacgattc tattggctta ggggaagatg
   104761 gtccgacgca ccaggccgtt gagcaactgg ccagtctgcg cttaacgccg aatttcagca
   104821 cctggcgacc gtgcgatcag gtggaagcgg cggtgggctg gaagctggcg gttgagcgcc
   104881 acaacgggcc gacagcactg atcctctcaa ggcagaatct ggcccaggtg gaacgtacgc
   104941 cggatcaggt taaagagatt gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc
   105001 cagatattat tctgattgcc accggttcag agatggaaat taccctgcaa gcggcagaga
   105061 aattagcagg agaaggtcgc aatgtacgcg tagtttccct gccctcgacc gatattttcg
   105121 acgcccagga tgaggaatat cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg
   105181 cggtggaagc aggtattgcc gattactggt acaagtatgt tggtctgaaa ggggtaattg
   105241 tcgggatgac gggttacggg gaatctgctc cggcggataa gctgttcccg ttctttggct
   105301 ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat
   105361 ggtgatcgcc ggatgctgat tgccggatgc gacgctgacg cgtcttatcc ggcctacagc
   105421 gtcttatccg gcctacatgt ccccgcaatt ttgtttaacg ggtgatccac aacgtgggcc
   105481 aggcgtctgg cccatgccag ttatcgcagg tgggttctgc ggcataacgc accaggcgaa
   105541 aacgctgacc gtcaaagcgc cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt
   105601 tcgctaaggt caccagttca cgcgatttct catcaaatgt tgcgttcatc agttccagtt
   105661 cattcgtctc ctgaccgttg ttgaatggca aacgcaaccg aaccgggcgc gaggctagtg
   105721 gctttttacg cgacacaatc catgccaaat caatggtgtt ataggcccct gcctcacagc
   105781 taatcatcat cagcgcttta tcatcagtca gcgcagtgac attcacctca cgacgtaacg
   105841 gatcaagcga gcagcgcaga ccattcatcc gccagtttcc ataatccagc aaatcgttgc
   105901 gttcttcgag tgagagtggc gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag
   105961 gcgcgggcgg tacgctgagc ggcggttcgt cccctttctt gatccacgcg gtttcactgc
   106021 caacgcgctt ttgctgagca tcaataaaca acaacgccgc ttttaagcca ctcagagaaa
   106081 tggtctgatt gccgtcgcgt aaggtgatcg ccttcccttc ctgaatcacc tgcaaaaacg
   106141 cggtgatggt tgccgtatca tcggttacta ataaccatgg tgaaatccgc cacttgtcac
   106201 cacttaacgc caacggctca ccatctaaca gcagccgtgg cgctatctct ccttcagacg
   106261 catccggcga cttcaacccg ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccgg
   106321 cgctgcggct cagggtcatc accagtccat tatgatcgcc cgtattgcgc gccacgcaga
   106381 agttttggtt attacaggtg acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc
   106441 acaccaaaga cgtcggcaac aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg
   106501 acggtacgac cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag
   106561 cgcggctcaa tcagatttgt cggattgagc caataataaa caacgaaata ttattgttat
   106621 ttttcagtcc attaaatgtg acgtttgcaa atagttaagc aataacaccg tcttaccgtc
   106681 acgtatctcg ccggttttga tcatctccag cgcctggctg aacggcagct cgagcacttc
   106741 aatatcttca tcttcaacac cgccaccggc gttagcgcgc tgattgtcac tgtattcggc
   106801 gataaaaaag tggattagct cagtcacacc gccaggcgac atatacagtt caaataattt
   106861 gcgcacttcg cccacttcat agcccgtctc ttcaatcgct tctttacgaa tgcacacttc
   106921 cggttcgtcg ttatccagca gcccggcgca ggtttcaatc agctgcccgc tttcattgcc
   106981 attaacccag gtagcgacac ggaactgacg aatcagaacc acgctctttt tcttcgcgtt
   107041 gtacaggagg atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac
   107101 ttcgccatct ttgcgcgtga gatcgtaggt aatgttgtgc agggtgaaat agttatcgga
   107161 gagaattttg tctttaatga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat
   107221 gaacaattat cttacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga
   107281 ctgattttac ccccagccag tcaataatcc cctgtgccgc atgacgtcct tctgccattg
   107341 cggtaaccac cagatccgca ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg
   107401 tggtctggta acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg
   107461 ttacaccgtg cgactccagc cacggcatcc cgtgtggatt gaagccaaac gccataatca
   107521 ccgcatcggc tggcatgaca aattcactgc cctcaatcgg tacaggtcgc cgacgcccct
   107581 gggcatccgg ctcgccaaga cgcgtgcgca ggaaacgaat cccgcagacg tgaccttgtt
   107641 cattcagctc aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcgcggg
   107701 cgttcctcac ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg
   107761 tgacgttact cgcgccgtgg cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc
   107821 cgcccagtac cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt
   107881 cgagccccat cacctgttta gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg
   107941 gcgcatcttc attgggtaaa cccgcgttca tggaacggta agtgcctacg ccaacgaaga
   108001 ccgcgtcgta ttgttccaaa agcgaatcca aagagacatc tttacccact tcacaattga
   108061 gttcgaagtg aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat
   108121 ccagtttgaa agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt
   108181 acaccgtcac cgccacgcca ttgcgggtca gaacatccgc acaggccagc cctgccggac
   108241 ctgccccgat aatcgccacc cgcttgtcca ctttggtgac atgacttaag tcaggacgcc
   108301 aacctttcgc caacgcctga tctgaaatat agcgttcaat attgccgata gttaccgcgc
   108361 cgtgctcatc gcgaatagta caggcacctt cacacaaacg gtcttgcgga caaacgcgtc
   108421 cggtaatttc cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc
   108481 cggctttcac cagttcaatc cactgcggaa tatggttatg cagcgggcag gtccattcac
   108541 aaacgctgtg ctcgccgcac ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc
   108601 gaaatggcag ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg
   108661 gctcgccacg cgcgggcgtt gcctgcattt gttcgacttt actcattacc ggcatttctt
   108721 gcgccgcggt actggcatgc cacggttgat gttcctgacg cgcggtgcgc aagcggcggg
   108781 gtttcgccat gccggagagt gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat
   108841 tctcaacaca ggcaggaccg ttttcgcgcc ccgcacaaag gtcgcattta tgcgccgtgg
   108901 cttttacttt tcctgccgcg acgggtgtca ggacgatttg catcgtacca aaaggacagg
   108961 ccaccacgca ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa
   109021 cgtggctgat tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac
   109081 aggtcactgc actacgttgc tgttgatgtt tgataaccgt aattcgggga tgaaaatggt
   109141 gttggctcag gacatgttgc tcatcattgt gagccatgac acaggcgatt tcacaagcat
   109201 gacaacccag acattgctga ctgttggcca taataaaacg attcataacg accttctttt
   109261 ttggttgtaa aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaat
   109321 cggcaatgtt catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa
   109381 taatcccatc actgactaaa ttgtgtttca gctaactgga acattaatga ttttttgtgg
   109441 agaagacgcg tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt
   109501 acattgtgct gctgtcgatt ctttccacgg gtatcgctct gctaactctg gcgagcagtt
   109561 tgcgcgacgc tgaggctatc aatattgccg gatcgctacg tatgcagagt taccgcctgg
   109621 gctacgactt gcaaagtggc agtccacaac tcaatgcaca tcgccagttg tttcagcagg
   109681 cactgcattc accggtatta accaatctca acgtctggta tgtgccagaa gcagtaaaaa
   109741 ctcgctatgc gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg
   109801 atttgccgtg gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc
   109861 tggctttaca gcactacgct gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg
   109921 gcggtatcgg tattttcacg ctggtctttt ttactttgcg ccgtatacgc catcaggtgg
   109981 ttgccccgct gaatcagctg gttaccgcca gtcaacgtat tgaacacggg cagttcgact
   110041 cgccgccgct ggataccagt ctgccgaatg agcttggtct gcttgcaaaa acctttaacc
   110101 agatgtcgag cgagctgcat aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga
   110161 cccgcgatct ccacgaggcc aagcgtcgtc tggaggtgtt gtatcaatgt tcccaggcgc
   110221 tgaacaccag ccagattgat gtgcattgtt tccgccatat tttgcagatt gttcgcgaca
   110281 atgaagcggc tgaatatctg gagttaaatg tcggtgaaaa ctggcggatt agcgaagggc
   110341 aaccaaaccc ggaattgccg atgcagattt taccggtgac aatgcaagag acggtttacg
   110401 gcgaactgca ctggcaaaat agtcacgttt catcatcaga accgctgctt aacagtgttt
   110461 catcgatgct gggacgcggt ttgtacttta atcaggcgca gaagcatttt cagcagttat
   110521 tgttgatgga agaacgtgca accatcgccc gcgaattgca cgactcgctg gctcaggtac
   110581 tttcttactt acgtatccag ttgacgttac tgaagcgttc gataccggaa gataacgcca
   110641 ccgcacaaag tatcatggcc gatttttccc aggcgttgaa tgatgcttat cggcagttac
   110701 gcgagctgtt gactactttc cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga
   110761 gggaaatgct ggatacgtta caaaatcaaa ccagcgccaa actgaccctc gactgccgtc
   110821 tgccaaccct ggcgctggat gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag
   110881 cggtgctgaa tgcgatgaag cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg
   110941 cgccggacgg caatcacacg gtttatatcc gtgataacgg gattggtatc ggtgaaccga
   111001 aagaacccga aggtcattat ggtctgaata tcatgcgcga acgcgcggaa cggctgggtg
   111061 ggacgctgac tttttcacaa ccttccggcg gcggcacgtt agtgagtatt agctttcgct
   111121 ctgcggaggg tgaggaaagt cagttgatgt aatgcctcct actgaccaaa gaatacttgc
   111181 acttaaggtt cagtataaaa gggcatgata atttacatta actccttttt ttctccacga
   111241 ttggctcgta ccttgccgct acagtgaagc aagtcaagcc tacaacgata cgcagaaaca
   111301 cgaggtcctc ttttaatggc gaatttcttt attgatcgcc ccatttttgc ctgggtgctg
   111361 gcaatcctgt tgtgtctgac agggaccctg gcgatttttt cattgcccgt tgaacaatac
   111421 cccgatctcg cgccaccgaa tgtgcgagtg accgctaact atcccggcgc atcggcccag
   111481 acgctggaaa acaccgtgac ccaggttatc gagcaaaata tgaccggcct cgataatctc
   111541 atgtatatgt catctcagag cagtggcacc ggtcaggcat ctgtcacttt aagttttaaa
   111601 gcaggcaccg atccggacga agccgtgcag caagtacaaa accagctgca atcagccatg
   111661 cgaaagttac cgcaggcggt gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc
   111721 aatattctga ccattgcttt cgtctctacc gatggttcga tggataaaca ggatattgcc
   111781 gattatgttg ccagtaatat tcaggacccg ttaagccgcg tgaatggcgt cggggatatc
   111841 gatgcctatg gttcgcaata ttccatgcgt atctggctgg acccggcgaa actcaacagt
   111901 ttccagatga cggctaaaga tgtcactgat gccattgagt cacagaacgc gcagattgcg
   111961 gttgggcaac ttggtggtac accttccgtc gataagcagg cgctcaacgc caccattaac
   112021 gcccagtcac tgctgcaaac accagaacag ttccgcgata tcaccttgcg ggtgaatcaa
   112081 gacggctcag aggtaaggct gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac
   112141 gattatctta gccgcttcaa tggtaagcca gcctccgggc tgggggtaaa actggcctcc
   112201 ggcgcaaacg aaatggcgac agcggagctg gtgctcaatc gtctcgacga gctggcgcag
   112261 tacttcccgc acggactgga atacaaggtg gcgtatgaaa ccacctcgtt tgttaaagcc
   112321 tccattgaag acgtggtgaa aacgctgctg gaagctatcg ccctggtttt cctcgttatg
   112381 tatctgttcc tgcaaaactt ccgcgccacg ctgataccca ctatcgccgt gccggtggtg
   112441 ttgatgggaa ccttctccgt actttacgcc ttcggttaca gcgtcaacac cttaaccatg
   112501 ttcgcgatgg tactggcgat cggtctgctg gtggatgacg caatcgtggt ggtggaaaac
   112561 gtcgaacgta ttatgagtga ggaaggactc actcctcgcg aagccacacg taaatcgatg
   112621 gggcagatcc agggggcact ggtcgggatt gcgatggttc tttcggcggt atttgtacca
   112681 atggccttct tcggcggcac caccggtgcc atctatcgcc agttctctat taccattgtt
   112741 gcggcgatgg tgctgtcagt actggtagcg atgatcctca ctccggctct gtgtgccaca
   112801 ctacttaagc cactgaaaaa aggtgagcat cacgggcaaa aaggcttttt tgcctggttt
   112861 aaccagatgt ttaaccgcaa cgccgaacgc tacgaaaaag gggtggcgaa aattctccac
   112921 cgtagcctgc gctggattgt gatttatgtc ctgctgcttg gcggcatggt gttcctgttc
   112981 ctgcgtttgc cgacgtcgtt cttaccgcag gaagaccgtg gcatgtttac tacctcggta
   113041 caattgccca gcggttcaac ccaacaacag accctgaaag tcgttgagca aatcgagaaa
   113101 tattacttca cccatgaaaa agacaacatc atgtcggtgt ttgccactgt tggttctggc
   113161 cccgggggta acgggcaaaa cgtggcgcga atgtttatcc gcctgaaaga ctggagcgaa
   113221 cgcgacagta agaccggcac ctcgtttgcc attatcgagc gtgcaacgaa ggcgtttaac
   113281 caaattaaag aagctcgcgt tatcgccagc agcccgccag caattagcgg tcttggtagt
   113341 tctgcaggtt ttgatatgga gttgcaggac cacgctggag cgggtcacga tgcgctgatg
   113401 gcagcacgta atcagttgct ggcgctggcg gcggaaaacc cggagctaac ccgtgtgcgc
   113461 cataacggcc tcgacgacag tccgcagttg cagattgata tcgaccagcg taaagctcag
   113521 gcgctgggcg ttgctatcga cgatattaac gacacactgc aaaccgcctg gggttcgagc
   113581 tatgtgaatg actttatgga tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg
   113641 ccgtatcgca tgctgccaga tgacattaat ctctggtatg tccgaaataa agatggcggc
   113701 atggtgccct tctctgcttt cgcgacctca cgctgggaaa caggctcgcc gcgtctggaa
   113761 cgctataacg gttattctgc ggttgagatt gttggggaag ccgcaccggg ggtcagtacc
   113821 ggtacggcga tggatattat ggaatcgtta gtgaagcagt tgccaaacgg ctttggtctg
   113881 gagtggacgg cgatgtcgta tcaggagcga ctttccggcg cgcaggctcc ggcgctgtac
   113941 gccatttcct tgctggtggt attcctgtgt ctggcggcgt tgtatgaaag ctggtcggtg
   114001 ccgttctcgg taatgctggt cgtgccgctg ggggttatcg gcgcgctgct ggcaacctgg
   114061 atgcgcgggc tggaaaacga cgtttacttc caggtgggcc tgttaacggt cattggttta
   114121 tcggcgaaaa acgccatcct gatcgtcgag tttgctaacg agatgaacca aaaaggccac
   114181 gacctgtttg aagcgacgct ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc
   114241 tcgctggcat ttatcttcgg cgtattgcca atggcaacca gcacgggtgc cggttccggt
   114301 ggtcagcatg cggtgggtac tggcgtaatg ggcgggatga tttcagctac tattctggct
   114361 atttacttcg tgccgctgtt ctttgtgctg gtgcgccgcc gcttcccgct gaagccgcgc
   114421 ccggaataag caataaaaaa ggcgacatgc caatgtgtcg cctttttcaa ctttccgatt
   114481 aagaacctgc tcagcgggtt cttgctgttt atactttgtc tcgggaatta cttacgaagc
   114541 ataacttcga taaagtcttt ccagttcccc agttcacgtt caatcataac aacctctctt
   114601 ataattatgg gtattctacg gaaacaatat accgtggtga agctaattta ctcgattgct
   114661 gcgatgacta cctccggggg acaaacctta tgtaaatact atggtcctgc ggtgataatt
   114721 tgtatgtgat acacagcaac atttcgagat attcatacgg catcta
//
LOCUS       DAZKGS010000002        95849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKGS010000002 DAZKGS010000000
VERSION     DAZKGS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..578
                     /gene="yfgH"
                     /locus_tag="ACHOQY_000110"
     CDS             60..578
                     /gene="yfgH"
                     /locus_tag="ACHOQY_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9676275.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            594..1133
                     /gene="yfgI"
                     /locus_tag="ACHOQY_000111"
     CDS             594..1133
                     /gene="yfgI"
                     /locus_tag="ACHOQY_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9676276.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1228..2805)
                     /gene="guaA"
                     /locus_tag="ACHOQY_000112"
     CDS             complement(1228..2805)
                     /gene="guaA"
                     /locus_tag="ACHOQY_000112"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9676277.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2874..4340)
                     /gene="guaB"
                     /locus_tag="ACHOQY_000113"
     CDS             complement(2874..4340)
                     /gene="guaB"
                     /locus_tag="ACHOQY_000113"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9676278.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4502..5872
                     /gene="xseA"
                     /locus_tag="ACHOQY_000114"
     CDS             4502..5872
                     /gene="xseA"
                     /locus_tag="ACHOQY_000114"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9676279.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5869..6084)
                     /gene="yfgJ"
                     /locus_tag="ACHOQY_000115"
     CDS             complement(5869..6084)
                     /gene="yfgJ"
                     /locus_tag="ACHOQY_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9676280.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6153..7625)
                     /gene="der"
                     /locus_tag="ACHOQY_000116"
     CDS             complement(6153..7625)
                     /gene="der"
                     /locus_tag="ACHOQY_000116"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9676281.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7743..8921)
                     /gene="bamB"
                     /locus_tag="ACHOQY_000117"
     CDS             complement(7743..8921)
                     /gene="bamB"
                     /locus_tag="ACHOQY_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9676282.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8932..9552)
                     /gene="yfgM"
                     /locus_tag="ACHOQY_000118"
     CDS             complement(8932..9552)
                     /gene="yfgM"
                     /locus_tag="ACHOQY_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9676283.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9570..10844)
                     /gene="hisS"
                     /locus_tag="ACHOQY_000119"
     CDS             complement(9570..10844)
                     /gene="hisS"
                     /locus_tag="ACHOQY_000119"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9676284.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10955..12073)
                     /gene="ispG"
                     /locus_tag="ACHOQY_000120"
                     /gene_synonym="gcpE"
     CDS             complement(10955..12073)
                     /gene="ispG"
                     /locus_tag="ACHOQY_000120"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9676285.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12100..13113)
                     /gene="rodZ"
                     /locus_tag="ACHOQY_000121"
     CDS             complement(12100..13113)
                     /gene="rodZ"
                     /locus_tag="ACHOQY_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9676286.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13398..14552)
                     /gene="rlmN"
                     /locus_tag="ACHOQY_000122"
     CDS             complement(13398..14552)
                     /gene="rlmN"
                     /locus_tag="ACHOQY_000122"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9676287.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14702..15133)
                     /gene="ndk"
                     /locus_tag="ACHOQY_000123"
     CDS             complement(14702..15133)
                     /gene="ndk"
                     /locus_tag="ACHOQY_000123"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9676288.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15282..17594)
                     /gene="pbpC"
                     /locus_tag="ACHOQY_000124"
     CDS             complement(15282..17594)
                     /gene="pbpC"
                     /locus_tag="ACHOQY_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9676289.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17595..22557)
                     /gene="yfhM"
                     /locus_tag="ACHOQY_000125"
                     /pseudo
     CDS             complement(17595..22557)
                     /gene="yfhM"
                     /locus_tag="ACHOQY_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            22764..23609
                     /gene="sseA"
                     /locus_tag="ACHOQY_000126"
     CDS             22764..23609
                     /gene="sseA"
                     /locus_tag="ACHOQY_000126"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9676290.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23945..24070
                     /gene="timP"
                     /locus_tag="ACHOQY_000127"
     CDS             23945..24070
                     /gene="timP"
                     /locus_tag="ACHOQY_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9676291.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24102..24878)
                     /gene="sseB"
                     /locus_tag="ACHOQY_000128"
     CDS             complement(24102..24878)
                     /gene="sseB"
                     /locus_tag="ACHOQY_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9676292.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25020..26303)
                     /gene="pepB"
                     /locus_tag="ACHOQY_000129"
     CDS             complement(25020..26303)
                     /gene="pepB"
                     /locus_tag="ACHOQY_000129"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9676293.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26481..26681)
                     /gene="iscX"
                     /locus_tag="ACHOQY_000130"
     CDS             complement(26481..26681)
                     /gene="iscX"
                     /locus_tag="ACHOQY_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9676294.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26693..27028)
                     /gene="fdx"
                     /locus_tag="ACHOQY_000131"
     CDS             complement(26693..27028)
                     /gene="fdx"
                     /locus_tag="ACHOQY_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9676295.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27030..28880)
                     /gene="hscA"
                     /locus_tag="ACHOQY_000132"
     CDS             complement(27030..28880)
                     /gene="hscA"
                     /locus_tag="ACHOQY_000132"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9676296.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28897..29412)
                     /gene="hscB"
                     /locus_tag="ACHOQY_000133"
     CDS             complement(28897..29412)
                     /gene="hscB"
                     /locus_tag="ACHOQY_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9676297.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29508..29831)
                     /gene="iscA"
                     /locus_tag="ACHOQY_000134"
     CDS             complement(29508..29831)
                     /gene="iscA"
                     /locus_tag="ACHOQY_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9676298.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29848..30234)
                     /gene="iscU"
                     /locus_tag="ACHOQY_000135"
     CDS             complement(29848..30234)
                     /gene="iscU"
                     /locus_tag="ACHOQY_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9676299.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30262..31476)
                     /gene="iscS"
                     /locus_tag="ACHOQY_000136"
     CDS             complement(30262..31476)
                     /gene="iscS"
                     /locus_tag="ACHOQY_000136"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9676300.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31717..32205)
                     /gene="iscR"
                     /locus_tag="ACHOQY_000137"
     CDS             complement(31717..32205)
                     /gene="iscR"
                     /locus_tag="ACHOQY_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9676301.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32506..33243)
                     /gene="trmJ"
                     /locus_tag="ACHOQY_000138"
     CDS             complement(32506..33243)
                     /gene="trmJ"
                     /locus_tag="ACHOQY_000138"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9676302.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33362..34165
                     /gene="suhB"
                     /locus_tag="ACHOQY_000139"
     CDS             33362..34165
                     /gene="suhB"
                     /locus_tag="ACHOQY_000139"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9676303.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34310..35164
                     /gene="yfhR"
                     /locus_tag="ACHOQY_000140"
     CDS             34310..35164
                     /gene="yfhR"
                     /locus_tag="ACHOQY_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9676304.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35355..36635
                     /gene="csiE"
                     /locus_tag="ACHOQY_000141"
     CDS             35355..36635
                     /gene="csiE"
                     /locus_tag="ACHOQY_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9676305.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36627..37766)
                     /gene="hcaT"
                     /locus_tag="ACHOQY_000142"
     CDS             complement(36627..37766)
                     /gene="hcaT"
                     /locus_tag="ACHOQY_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9676306.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37926..38816)
                     /gene="hcaR"
                     /locus_tag="ACHOQY_000143"
     CDS             complement(37926..38816)
                     /gene="hcaR"
                     /locus_tag="ACHOQY_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP9676307.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38952..40313
                     /gene="hcaE"
                     /locus_tag="ACHOQY_000144"
     CDS             38952..40313
                     /gene="hcaE"
                     /locus_tag="ACHOQY_000144"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP9676308.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40310..40828
                     /gene="hcaF"
                     /locus_tag="ACHOQY_000145"
     CDS             40310..40828
                     /gene="hcaF"
                     /locus_tag="ACHOQY_000145"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP9676309.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40828..41148
                     /gene="hcaC"
                     /locus_tag="ACHOQY_000146"
     CDS             40828..41148
                     /gene="hcaC"
                     /locus_tag="ACHOQY_000146"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP9676310.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41145..41957
                     /gene="hcaB"
                     /locus_tag="ACHOQY_000147"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41145..41957
                     /gene="hcaB"
                     /locus_tag="ACHOQY_000147"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP9676311.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41967..43169
                     /gene="hcaD"
                     /locus_tag="ACHOQY_000148"
     CDS             41967..43169
                     /gene="hcaD"
                     /locus_tag="ACHOQY_000148"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP9676312.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43266..43688
                     /gene="yphA"
                     /locus_tag="ACHOQY_000149"
     CDS             43266..43688
                     /gene="yphA"
                     /locus_tag="ACHOQY_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9676313.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43736..44608)
                     /gene="yphB"
                     /locus_tag="ACHOQY_000150"
     CDS             complement(43736..44608)
                     /gene="yphB"
                     /locus_tag="ACHOQY_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9676314.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44620..45714)
                     /gene="yphC"
                     /locus_tag="ACHOQY_000151"
     CDS             complement(44620..45714)
                     /gene="yphC"
                     /locus_tag="ACHOQY_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9676315.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45747..46745)
                     /gene="yphD"
                     /locus_tag="ACHOQY_000152"
     CDS             complement(45747..46745)
                     /gene="yphD"
                     /locus_tag="ACHOQY_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9676316.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46770..48281)
                     /gene="yphE"
                     /locus_tag="ACHOQY_000153"
     CDS             complement(46770..48281)
                     /gene="yphE"
                     /locus_tag="ACHOQY_000153"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9676317.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48304..49287)
                     /gene="yphF"
                     /locus_tag="ACHOQY_000154"
     CDS             complement(48304..49287)
                     /gene="yphF"
                     /locus_tag="ACHOQY_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9676318.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49384..52665)
                     /gene="yphG"
                     /locus_tag="ACHOQY_000155"
     CDS             complement(49384..52665)
                     /gene="yphG"
                     /locus_tag="ACHOQY_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9676319.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52783..53976
                     /gene="yphH"
                     /locus_tag="ACHOQY_000156"
     CDS             52783..53976
                     /gene="yphH"
                     /locus_tag="ACHOQY_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9676320.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54040..55293)
                     /gene="glyA"
                     /locus_tag="ACHOQY_000157"
     CDS             complement(54040..55293)
                     /gene="glyA"
                     /locus_tag="ACHOQY_000157"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9676321.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55621..56811
                     /gene="hmpA"
                     /locus_tag="ACHOQY_000158"
     CDS             55621..56811
                     /gene="hmpA"
                     /locus_tag="ACHOQY_000158"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9676322.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56856..57194)
                     /gene="glnB"
                     /locus_tag="ACHOQY_000159"
     CDS             complement(56856..57194)
                     /gene="glnB"
                     /locus_tag="ACHOQY_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9676323.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57255..58589)
                     /gene="glrR"
                     /locus_tag="ACHOQY_000160"
     CDS             complement(57255..58589)
                     /gene="glrR"
                     /locus_tag="ACHOQY_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9676324.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58579..59292)
                     /gene="qseG"
                     /locus_tag="ACHOQY_000161"
     CDS             complement(58579..59292)
                     /gene="qseG"
                     /locus_tag="ACHOQY_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9676325.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59457..60884)
                     /gene="qseE"
                     /locus_tag="ACHOQY_000162"
     CDS             complement(59457..60884)
                     /gene="qseE"
                     /locus_tag="ACHOQY_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9676326.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61460..65347)
                     /gene="purL"
                     /locus_tag="ACHOQY_000163"
                     /gene_synonym="purI"
     CDS             complement(61460..65347)
                     /gene="purL"
                     /locus_tag="ACHOQY_000163"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9676327.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65605..67161
                     /gene="mltF"
                     /locus_tag="ACHOQY_000164"
     CDS             65605..67161
                     /gene="mltF"
                     /locus_tag="ACHOQY_000164"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9676328.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67158..67661)
                     /gene="tadA"
                     /locus_tag="ACHOQY_000165"
     CDS             complement(67158..67661)
                     /gene="tadA"
                     /locus_tag="ACHOQY_000165"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9676329.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67719..68354)
                     /gene="yfhb"
                     /locus_tag="ACHOQY_000166"
     CDS             complement(67719..68354)
                     /gene="yfhb"
                     /locus_tag="ACHOQY_000166"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9676330.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68563..69411
                     /gene="yfhH"
                     /locus_tag="ACHOQY_000167"
     CDS             68563..69411
                     /gene="yfhH"
                     /locus_tag="ACHOQY_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9676331.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69467..69727
                     /gene="yfhL"
                     /locus_tag="ACHOQY_000168"
     CDS             69467..69727
                     /gene="yfhL"
                     /locus_tag="ACHOQY_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9676332.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69921..70001)
                     /gene="shoB"
                     /locus_tag="ACHOQY_000169"
     CDS             complement(69921..70001)
                     /gene="shoB"
                     /locus_tag="ACHOQY_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9676333.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70421..70801)
                     /gene="acpS"
                     /locus_tag="ACHOQY_000170"
     CDS             complement(70421..70801)
                     /gene="acpS"
                     /locus_tag="ACHOQY_000170"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9676334.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70801..71532)
                     /gene="pdxJ"
                     /locus_tag="ACHOQY_000171"
     CDS             complement(70801..71532)
                     /gene="pdxJ"
                     /locus_tag="ACHOQY_000171"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9676335.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71544..72272)
                     /gene="recO"
                     /locus_tag="ACHOQY_000172"
     CDS             complement(71544..72272)
                     /gene="recO"
                     /locus_tag="ACHOQY_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9676336.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72284..73189)
                     /gene="era"
                     /locus_tag="ACHOQY_000173"
     CDS             complement(72284..73189)
                     /gene="era"
                     /locus_tag="ACHOQY_000173"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9676337.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73186..73866)
                     /gene="rnc"
                     /locus_tag="ACHOQY_000174"
     CDS             complement(73186..73866)
                     /gene="rnc"
                     /locus_tag="ACHOQY_000174"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9676338.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74139..75113)
                     /gene="lepB"
                     /locus_tag="ACHOQY_000175"
     CDS             complement(74139..75113)
                     /gene="lepB"
                     /locus_tag="ACHOQY_000175"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9676339.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(75129..76928)
                     /gene="lepA"
                     /locus_tag="ACHOQY_000176"
     CDS             complement(75129..76928)
                     /gene="lepA"
                     /locus_tag="ACHOQY_000176"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9676340.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77126..77605)
                     /gene="rseC"
                     /locus_tag="ACHOQY_000177"
     CDS             complement(77126..77605)
                     /gene="rseC"
                     /locus_tag="ACHOQY_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9676341.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77602..78558)
                     /gene="rseB"
                     /locus_tag="ACHOQY_000178"
     CDS             complement(77602..78558)
                     /gene="rseB"
                     /locus_tag="ACHOQY_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9676342.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78558..79208)
                     /gene="rseA"
                     /locus_tag="ACHOQY_000179"
     CDS             complement(78558..79208)
                     /gene="rseA"
                     /locus_tag="ACHOQY_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9676343.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79241..79816)
                     /gene="rpoE"
                     /locus_tag="ACHOQY_000180"
     CDS             complement(79241..79816)
                     /gene="rpoE"
                     /locus_tag="ACHOQY_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9676344.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79813..79968)
                     /gene="rseD"
                     /locus_tag="ACHOQY_000181"
     CDS             complement(79813..79968)
                     /gene="rseD"
                     /locus_tag="ACHOQY_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9676345.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80224..81846
                     /gene="nadB"
                     /locus_tag="ACHOQY_000182"
     CDS             80224..81846
                     /gene="nadB"
                     /locus_tag="ACHOQY_000182"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9676346.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81831..82568)
                     /gene="trmN"
                     /locus_tag="ACHOQY_000183"
     CDS             complement(81831..82568)
                     /gene="trmN"
                     /locus_tag="ACHOQY_000183"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9676347.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82700..84034
                     /gene="srmB"
                     /locus_tag="ACHOQY_000184"
     CDS             82700..84034
                     /gene="srmB"
                     /locus_tag="ACHOQY_000184"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9676348.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84244..85125)
                     /gene="yfiE"
                     /locus_tag="ACHOQY_000185"
     CDS             complement(84244..85125)
                     /gene="yfiE"
                     /locus_tag="ACHOQY_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9676349.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            85228..85815
                     /gene="eamB"
                     /locus_tag="ACHOQY_000186"
     CDS             85228..85815
                     /gene="eamB"
                     /locus_tag="ACHOQY_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9676350.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(85871..86254)
                     /gene="grcA"
                     /locus_tag="ACHOQY_000187"
     CDS             complement(85871..86254)
                     /gene="grcA"
                     /locus_tag="ACHOQY_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9676351.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86559..87248
                     /gene="ung"
                     /locus_tag="ACHOQY_000188"
     CDS             86559..87248
                     /gene="ung"
                     /locus_tag="ACHOQY_000188"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9676352.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87296..88333)
                     /gene="yfiF"
                     /locus_tag="ACHOQY_000189"
     CDS             complement(87296..88333)
                     /gene="yfiF"
                     /locus_tag="ACHOQY_000189"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9676353.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88540..88959
                     /gene="trxC"
                     /locus_tag="ACHOQY_000190"
     CDS             88540..88959
                     /gene="trxC"
                     /locus_tag="ACHOQY_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9676354.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            89028..89726
                     /gene="tapT"
                     /locus_tag="ACHOQY_000191"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             89028..89726
                     /gene="tapT"
                     /locus_tag="ACHOQY_000191"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9676355.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89758..92418
                     /gene="pat"
                     /locus_tag="ACHOQY_000192"
     CDS             89758..92418
                     /gene="pat"
                     /locus_tag="ACHOQY_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9676356.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92532..93887
                     /gene="pssA"
                     /locus_tag="ACHOQY_000193"
     CDS             92532..93887
                     /gene="pssA"
                     /locus_tag="ACHOQY_000193"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9676357.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93933..94256
                     /gene="yfiM"
                     /locus_tag="ACHOQY_000194"
     CDS             93933..94256
                     /gene="yfiM"
                     /locus_tag="ACHOQY_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9676358.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(94253..95551)
                     /gene="kgtP"
                     /locus_tag="ACHOQY_000195"
     CDS             complement(94253..95551)
                     /gene="kgtP"
                     /locus_tag="ACHOQY_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9676359.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22853 a        26118 c        24102 g        22776 t
ORIGIN      
        1 ctttcctctt ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag
       61 tgatgaaatt taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag
      121 gatgccagtc aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata
      181 ccaaacaaga aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag
      241 acaactccca aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg
      301 gcggtgttat cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag
      361 ttggcggcgg agctgtaggc gcggcagtgg gttctatggt gaatgataaa accttagtgg
      421 aaggtgtttc tttaacatat aaggaaggca ccaaagtgta tacctctacc caggtgggta
      481 aagagtgcca gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc
      541 gtattcagcc aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga
      601 aagtttttct ttgcgccatc ttggcctcct taagctatcc ggctatcgcc tcatcattgc
      661 aggatcaact ctctgctgta gcagaagctg aacagcaagg taaaaatgaa gagcaaaggc
      721 agcatgacga atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg
      781 caaatgccca ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc
      841 gtcaggataa actggacgcc gaagcctctg cggacaaaaa acgcgatcaa agttatgaag
      901 atgagctacg cagcttagag attcagaaac aaaaactggc gctggcaaaa gaagaagccc
      961 gcgttaagcg agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg
     1021 atgtggtgca atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga
     1081 tgaaacgcgt gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt
     1141 tagtaacttc aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt
     1201 tgttcattca tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc
     1261 gctgatgtca tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac
     1321 gcgaccgagg aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt cgatggtttc
     1381 gacagcacgc agagagacaa cccaatcata cttacgacca tcgcccatta cgccaacgga
     1441 acgtaccggc aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg
     1501 cagttcttca atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac
     1561 ttcacccagc acacgaacgc caaggcctgg tcccgggaac gggtgacggt acagcatgtc
     1621 gtacggcagg cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag
     1681 cggttcaacc aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt
     1741 gatgacgtgt gctttaccgg ttgcagaagc cgcagattcg ataacgtcag ggtagatggt
     1801 gccctgcgcc agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac
     1861 gaaaacgcgc ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga
     1921 caggaagcga tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat cgccaaacat
     1981 atccagaacc tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt
     2041 caggttttta ccgatagcgc ggtgcagcag cattgcggtt acggaggaat ccacaccacc
     2101 agagaggccg aggatgactt tatcgtcgcc tacctgctcg cggatgcgag ctacagcatc
     2161 gtcgataatt ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg
     2221 ctccagcatg cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata
     2281 gaagcgtttt tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt
     2341 gacgaagtcg gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag
     2401 cggtttaccg tctgcggtca gcgcatcttc gataccgcga accagtgcgc tgtcgtttac
     2461 gacttcaacc tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa
     2521 ctgcattgcc atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa
     2581 gacatactgc ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc cgccggaaag
     2641 aataatgccg cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag
     2701 ttcgcagtaa acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc
     2761 gaagtccaga atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga
     2821 ggcaagtgaa acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag
     2881 cccagacggt agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga
     2941 atgcccgcac cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca
     3001 cagccggtca gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc
     3061 aggcgacctt tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg
     3121 ttatcgctct ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata
     3181 ccacggtaag atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca
     3241 gtacccgcca gcatggaacc taccatcacc gcgcttgcgc cagctgcgat agctttggcg
     3301 atgtcgccgg agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg
     3361 gcttcaactg cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga
     3421 gtcgtacaga tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc
     3481 aaagcgcgtg cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat
     3541 ttagcacggg tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg
     3601 atcagcagaa cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca
     3661 cctgcgccaa ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt
     3721 ttacgttccg ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca
     3781 tcaaccacca gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg
     3841 gcttcacctt cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc
     3901 tggttcaggt cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt
     3961 tcttcggtaa cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg
     4021 cgcagcgtcg tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt
     4081 ttcacacgac gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg
     4141 ataccgcctt cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct
     4201 gcggaaagca tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca
     4261 gtattcggca gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct
     4321 tctttagcga tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc
     4381 ggcattatac agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc
     4441 cccgaagggc gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca
     4501 catgttacct tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg
     4561 tctgctgctt gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac
     4621 acaaccggct tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg
     4681 cgcgatgttc cgcaacagca accgtcgggt gaccttccgt ccacagcatg ggcaacaagt
     4741 tttagttcgc gccaatatta cgctctacga gccgcgcggc gactaccaga taatcgttga
     4801 gagtatgcag ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa
     4861 gttgcaggct gaaggattgt tcgatctgca atacaaaaaa ccacttccct cccctgcgca
     4921 ttgcgttggt gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt
     4981 aaaacgtcgc gatccttctc taccggtgat catctacccc accgccgttc agggcgatga
     5041 cgcgccgggg caaactgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt
     5101 gttgatcgtt gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg
     5161 cgtagcgcgg gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac
     5221 ggatgtgacc attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc
     5281 tgaagtagtg agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg
     5341 gctggagatg gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca
     5401 tcaccgatta cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga
     5461 gcgcctgcaa aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca
     5521 gcagcagcgg ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc
     5581 gcaaacgcgc attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag
     5641 cgccacgcgt gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc
     5701 aacgctggcg cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt
     5761 gaagcaagtt aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag
     5821 tgaagtaaaa aacatccagc cggtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac
     5881 gaattcaacg cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc
     5941 accgcaggct tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc
     6001 aataatttcc ccgcaggatg ggcaacgcgc atggtcgtta tcctgatcaa gcacatgctg
     6061 acattgtgga caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata
     6121 gagggaatgg cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc
     6181 agacgcttac gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg
     6241 ttttcccctt ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg
     6301 aagtagttca tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga
     6361 atcaccacaa tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga
     6421 ccgcgtacca gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata
     6481 gaggtcccca cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta
     6541 ccaacaccac tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc
     6601 agacggaagt ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac
     6661 ttattcacca caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga
     6721 tcctgatcgg aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggcatcttca
     6781 atggcctgca acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt
     6841 ttacgtacgc cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg
     6901 tagatactgt cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga
     6961 atacggttag tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc
     7021 ggcagacttt gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca
     7081 aattgcgccc agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc
     7141 cacggcagca gcacatgctc cagcagactt aatacgccac gaccgtgaga cgcggcgatc
     7201 gggtagattt cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga
     7261 ccgtcagttt tgtttgccac caggaaggtc ggtttttcac gggagcgcag atgtttggca
     7321 atcgcttcat ctgccggcat caggcccgcg cgcgcatcca ccataaacag tacgacgtcc
     7381 gcttcttcaa tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg
     7441 ccatcaatcc cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg
     7501 tacttacggt cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt
     7561 agacggttaa ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt
     7621 accatgttta aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt
     7681 tgttaaaaac aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac
     7741 gattaacgtg taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca
     7801 gcggcaaccg gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga
     7861 ccatcttcga cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca
     7921 ttatacagca ccggagaagt cagcaggcga tgcaacagat cgctttgtgt ccacagcgta
     7981 acgccgccat caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga
     8041 ttgccgtcga cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca
     8101 ctgcgcagat caagcgccgt caggttacca ttataggcca gcgcgaaaac aacgccgtta
     8161 acaacgacgg gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc
     8221 tgggaaatac gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga
     8281 ccattatcgc cacccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc
     8341 aaagagagcg aaggcatatc gaggttaact gtccatttga cagcgccgtc agcctcgttc
     8401 agcgcttgta actgaccgtt actggtgtgg attaacacca gaccgtcgct gaccaccggg
     8461 cgcgaaagtg cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc
     8521 agcgcgtaaa cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaga
     8581 ccgccagaaa gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca
     8641 gaccagattt ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct
     8701 gcatagacaa cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg
     8761 ctaccaacgg aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat
     8821 ggggacatct ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta
     8881 acggaaagca gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga
     8941 caaattatta attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac
     9001 gcctgcttcc catgcactac gcgcaccttg cttatcacct ttgctcagca atgcttcacc
     9061 acgcaggtcg gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag
     9121 cgcggcatca gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc
     9181 tttgagattt tcatcgctcg tgtctgccag cccctgttgt aactgggcgg cagctttctc
     9241 tagttcattt ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt
     9301 atttttattt tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct
     9361 cactgcggta accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg
     9421 gctgttccag tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag
     9481 tgctttgcca ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc
     9541 gtaaatttcc acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct
     9601 acgctatcct gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg
     9661 ccgttagcca cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca
     9721 cgggcaaact gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt
     9781 aattcatcac gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa
     9841 gccaccaggt atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact
     9901 aacaatacaa gacgttcgag gcccatcgca aaaccgacag ccggtgttgc acgaccgccc
     9961 agttgttcca caagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgaga
    10021 ctgttagtca cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc
    10081 tggtttacgg tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc
    10141 tcacgagatt cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc
    10201 acttccggat tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag
    10261 tcttcgtcca gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg
    10321 gcgcgtgctt ccagcgaacc gatagaattc agctcaagag ttacgtgttc ggaaataccc
    10381 agcgcgcgcc accagcgggc ggtgagcata atcagttcag cgtcgatatc cggaccttgc
    10441 agaccgaaaa cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc
    10501 tcgtgacgga acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca
    10561 tgctcgatgc cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg
    10621 ccattgcgat cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc
    10681 gcacgtttga atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag
    10741 ctgccgagca cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc
    10801 aggtaatcgt tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg
    10861 aatataaaaa tgaaccctca acgcttccct caatgtttcg ggagctatgc gggttcaatc
    10921 atacacggga agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa
    10981 ttcgacgcgc ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga
    11041 tcatatcgtt gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct
    11101 tgttgccgcc ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc
    11161 agccgataat cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt
    11221 taaccgtacc gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttga
    11281 tcccgcgcga acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga
    11341 ccggatcggc cgccagtgat acgcgcagcg tgtcgccgat gccttcagac agcagcagac
    11401 ctaaaccaat ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca
    11461 gatgcagcgg ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga
    11521 cgtcagacgc tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa
    11581 catggcgcat ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca
    11641 gatctttttc cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg
    11701 cacagtcaac caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca
    11761 gacaatcgac gccgtattcc gctactttca gtgcaatgcg atagtcgaag tggatgtcag
    11821 ccaccagcgg cacgttaacc tgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg
    11881 tcggtacgga gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga
    11941 ccgttgcttc gacgtctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac
    12001 catcgccaat cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag
    12061 cctggttatg catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc
    12121 ggcattgagg gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg
    12181 tttcccttga tactggatct gtactgcggc tggcgcacca attttcagtt tgtatggtgc
    12241 ctggccggtt aagttcaaat taccgtcttt acgctgcata ccgctaaaca attttttacc
    12301 ggtagcatca gtaacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg
    12361 atcagccgcc ggcgtagtca cgcctgcctg atcggttggc aacggcgcag cgccatctgg
    12421 cgttgttgtt gccgttggtg ccggggtcgc ggcggtatca acatttgcct gcgaaggcga
    12481 aacaaccgca ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg
    12541 cgtgttggtt gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt
    12601 agttgtcgac gtatttaacg gaacactctg cccctgctcg ctattactgc tcagttccgc
    12661 cgaagattga tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca
    12721 ccaggcaccg ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca
    12781 gccgtcacgt tttttgcggc gtttaccgag ggaaaaactc tgcataggcg caacttttgc
    12841 agcccgtagt ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac
    12901 cagacgcgca taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc
    12961 cttatcttct tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc
    13021 ctgctgacta agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc
    13081 ttcattttgg tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat
    13141 tgagatgccg gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt
    13201 tatcatacag tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa
    13261 cttactgttg catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga
    13321 ccagacaatt gcaacttaat acttcattca taaaagatta cgccacggta cataaagtaa
    13381 ccgtggcgta atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta
    13441 cgcagggtac gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg
    13501 atatcatcac cacgggtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt
    13561 gagaaacggt cgatacggct gttcgagctg cgtccatacg gcgcgcccgg gaaggggttc
    13621 cacgggatca ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg
    13681 tgttcagtgc cgtcgttaac gtggtcaagc atcacatact caatagtgac acggccctga
    13741 ttggcgttgg atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac
    13801 tttttgttga tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt
    13861 gccagtgcaa cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta
    13921 gaaagcgtga cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc
    13981 gccggaacga cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta
    14041 gtgattgggc gctgaccggt gacttttgct gcgccgacga ttttcgccgc acgccacacc
    14101 tggccaataa tttccgatac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag
    14161 aatttacact ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct
    14221 tccgggatat acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg
    14281 ccgtcggatg aacgctgttc ttcaaccact tccggtgcgc ggatttccgc cacctctttc
    14341 agtttgccgc gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag
    14401 tgatacatcc acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa
    14461 aactcccgca tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg
    14521 acgttttcag gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt
    14581 tatggcccct ggaggattga aaaaagaaac gccccggtga gcggctgctc gtccgggggc
    14641 gctgcattgt acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt
    14701 attaacgggt gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt tcgcgagcgg
    14761 cagattcgac ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag
    14821 cgcgcagagt accagccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac
    14881 gctgaacggc gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt
    14941 caaccagacc atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgtgcctgtt
    15001 caacggtcag gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg
    15061 caaagatatt accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac
    15121 gttcaatagc catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag
    15181 atggcgcgga ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt
    15241 aaaataaaat gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc
    15301 actgtcgcga tttgccccac atcatccatc accagcaatt gataatcacc tttatccgtc
    15361 aaatgcaggg tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag
    15421 cgttcacctg cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa
    15481 cgtttaataa tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga
    15541 ccgtatgacg ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg
    15601 ggcagccagg gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg
    15661 caatcagcgg caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta
    15721 atgccttcct gctccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt
    15781 gccaggcggc ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag
    15841 ataacgccac gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac
    15901 agtaagatat tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca
    15961 acaacgggcg tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc
    16021 ccaatcgccc aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg
    16081 acgcgcggca aggcactatc cggcaagggt ttcgcttcat cagccataat ccggcgaatg
    16141 atccacgccg cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag
    16201 cgcaatttgc ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc
    16261 agttttgcac cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc gttgggcaaa
    16321 tataacggca atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc
    16381 agcacctgca cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg
    16441 ccatgaaaac cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct
    16501 tgcagcagtg atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt
    16561 ttgagcaccg atcctggcga tcggttcgca ttgaccatat caacatgacc aaagcgtgaa
    16621 tcatcgttga gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc
    16681 acgatcatcg ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct
    16741 tccagacgtc gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta
    16801 ccgagcatca tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag
    16861 atgggttctt cccttgactc ttttacctgc tcacgggacc acgcaccttg cacggccatc
    16921 cgttcgagca ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga
    16981 cggctgggcg cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca
    17041 ggcgactttc cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac
    17101 ggagcgcggt taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc
    17161 aacgcgcgcc agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga
    17221 cgagcaacct gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct
    17281 tgccatgctg cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga
    17341 tcttcataat tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga
    17401 taacgccaga tgccgtcagc atcggcgaag cgccatagcg gcgtaccatc ctgcgccacg
    17461 accactcgtg cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac
    17521 gctgcaagaa aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt
    17581 aacaagcgag gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc
    17641 cattggggaa catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc
    17701 accgcccgcg ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc
    17761 acaaagcgat cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt
    17821 aagttctgca cttcgccacc actttgctcc aggctggcgc tatcgttcgc cagattctgg
    17881 ttttccagtt ccagacccgc aggcagcaga tccacgacca acgcatccgg cacgctgtta
    17941 ctggctttta cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat
    18001 ttgctcttac catcagtacc aagaatatga cgctcgattt gcagcacatc gttcgccggt
    18061 aaaggtgcgg attgcggata accgctggca tccacacgca gccataacgg ctgatcgcca
    18121 ctgtttctca cctgcaaggt ggcaagttga tcgctattca gattgctgtt tagcgttttc
    18181 tcgcctgtca gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc
    18241 tgaatcgtac gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca
    18301 ccaaatgcct gctgcgaaag cgtgttcagc aaagtgttct gctcatcggg tagcagttta
    18361 ttttcttcca gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc
    18421 agccatatcc gctcatcact atttctcggc gttttcagcg ccagcacaat cgcttcttca
    18481 ccacgcgttg catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca
    18541 gaagcggcat ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc
    18601 tgacgggcca gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta
    18661 tccgcgtacg ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta
    18721 ccccggttaa tggcatctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa
    18781 tccatcacgt aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag
    18841 ccgccgttat cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg
    18901 cgtttctcat cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa
    18961 agtgacggga acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac
    19021 tctttgatat aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc
    19081 agcgtaacag gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc
    19141 tgtaacgccg taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc
    19201 cactgcttat gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc
    19261 tgaatttctc catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc
    19321 acacctggcg ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg
    19381 gcggtcaggg caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc
    19441 agacgcgagg tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt
    19501 gcggcaacta tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc
    19561 atgacgcgca attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt
    19621 tcgttgagcg ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt
    19681 ttaccaccac gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc
    19741 tgaccttcaa taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca
    19801 aagaacgcct gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca
    19861 acggcagaca ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta
    19921 attttcacgg ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg
    19981 aggcgacggt tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc
    20041 gcggagcgag atttatcgcc aggacgtacc accagcgtac ttaaatacag atcatgacga
    20101 ttccaggttt tatcgaccgg aatcgtcaga tccagccctt gagcccgaac atcaatctct
    20161 tgccaccaca gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc
    20221 gcggcgatat gcaacttaat ggtgtcgcca gggcgataac tggctttatc cagtttcagc
    20281 gtgacacggt cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca gctatagcca
    20341 gcccagaaac gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg
    20401 ccccactcta ccggaaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt
    20461 tcattttcga tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag
    20521 ttccagtagt aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt
    20581 ttcacgcctt gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca
    20641 atgggctgtt tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga ggcgaactgc
    20701 ggacggatcc ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc
    20761 gggcgaccgc ccgattccag caaactaccc tggaaaataa cctgtaatgg ggaatgcgtt
    20821 tccttccact ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga
    20881 acttcatcca gcgtgcggga aagattttcg gcagctatat cgccgaattc aaaaccaggt
    20941 aaggctgaca cagcttcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca
    21001 ttagcaggtg caccatacag gtagtacccc accacggaga atttcacttc atctttcggc
    21061 gttagcgggg ttttctcacc ggtcagattc agcgccatgc gctctggcat aaaatcttcg
    21121 acgtggaaat cccacatccg atactggtta tcgcccgtgt tagcgcgaat atgccacata
    21181 ccggttgccg cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga
    21241 ctaacgacgc tcctgagtac ctgcccatcg ggtttaatca cgtctaactt gatgggttga
    21301 ttgggcaacg ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcactt
    21361 gggcgataaa gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca
    21421 gcaatgttaa attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga
    21481 ccgtctttac gcgccaacag taatgccgcg tttttatcat tttccagctg cacatgcccc
    21541 tgagcgtcac tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca
    21601 attccttgct gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga
    21661 taacggtgag ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa
    21721 tcgtaacgtc cagcctgatt catcacagcc agatacacgc ccgcctgctg aaggcggttt
    21781 gatatcgccc agcggcagca ataatttttc acgggtgtta cgcgcaggat tgagatcaaa
    21841 ccgtccggtg tagaccagat ccgccatctg cagcagtttg tctgactgcc agttcgccag
    21901 cgaattgcgg tattcccatt ggctaatgaa tgctggcaga gattctggct taacgcggaa
    21961 gaagttaaca tcgacattat taacgttgag cgccattacc ggcagccctt caacgacttt
    22021 gccaggcagc agcgaaccac ggctggcaaa accgacgctg ggttggatgt cgcgggtagt
    22081 tatagttttt tcgtaatctt tactgaaggt tgcgttgttg agtactttga cctccttgcc
    22141 aatagtaacg atcaaatcac gtttcggttc gaggtggcgt aaacgtaact ctttaagatt
    22201 atctgacagc tcccaggcac catccacttt gccgcttttt ttatcgacga catgaataac
    22261 gcgtgagaaa tcctgatccg ggtcgagagg gatggagaac gtcagcacca gcgtggctgc
    22321 accatcaagt tggacttcag agagatccag caatgtcagc accttaccgg cactctgttg
    22381 ggccagtttt tgtctctccg gcacggagag ttttgctgaa ctcgcgtttt ctgaagaggc
    22441 cgctttagta acttcagaag gcgcatcttt tttcaccgct gttggcgcgt tatcgttgtt
    22501 gtcgcaccct gccagcgcca gcattagcat gcaggcggct acgcgtaact ttttcatttt
    22561 tcatccctgc cacaatggcc cgttagcaac gtcgaataat tattatgcgt gagaatttac
    22621 gttatgtaat tcagtatcac gcccatacaa tccgaatttt agtgagaatt gtggctcaga
    22681 cagccaaatc agcctctttc gcttgtcgct tttcactaaa catccgacaa tttagcctaa
    22741 ccccggcaaa aatggagatg cctatgtcca cgacatggtt tgtaggagcc gactggctcg
    22801 ccgaacatat tgatgacccg gaaattcaga ttattgatgc ccgcatggcg tcgcctggac
    22861 aggaagatcg taacgttgct caggagtatc tgaatggaca tattcccggc gcagtgtttt
    22921 ttgatatcga agcgctttct gatcacactt ccccgcttcc gcacatgctg ccgcgcccgg
    22981 aaacgttcgc cgtggcgatg cgtgaattag gcgttaacca ggataagcac ctgattgtct
    23041 atgacgaagg taatcttttc tcagccccac gagcatggtg gatgctgcgc acctttggtg
    23101 tagagaaagt gtcgattctg gggggtggac ttgcaggctg gcagcgcgat gatctgctgt
    23161 tagaagaagg tgcagtagag ctgccggaag gagagtttaa cgccgcgttt aatcctgaag
    23221 ccgtggtgaa agtaaccgat gtattattgg caagccatga aaatacggcg caaattattg
    23281 atgcccgccc ggctgcacgt tttaacgcag aagttgatga acctcgccca ggtttacgtc
    23341 gcggacatat tcccggagca ctgaatgttc cgtggacgga actggtgcgc gaaggcgaac
    23401 taaaaacgac cgatgaactg gatgcgatat tttttggtcg cggcgtcagc tacgacaaac
    23461 caattatcgt cagctgcggc tctggtgtaa cggcagccgt ggttttgtta gcactcgcga
    23521 cgctggatgt gccaaacgtg aaactgtacg acggcgcatg gagtgaatgg ggcgcgcggg
    23581 cagatttacc ggttgagcca gtgaaataag tattttacag gcaataaaaa accgccgaat
    23641 ttggcggttt tttattgctg gtctggttcg cggcctttcc agcaggttgt attaccgtag
    23701 taatgcaagc gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa
    23761 aagagagtta ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc
    23821 aagcgggcgc tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc
    23881 gcttgctatc gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg
    23941 cacaatgaag atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca
    24001 agtaaataat aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag
    24061 cagcaagtaa gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttctgt
    24121 ttaaagtcac gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta
    24181 ataaagtggc taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg
    24241 ccaggtaatg tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca
    24301 tcggcttcaa tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa
    24361 ataaaagcac gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt
    24421 tgtgctggcg gctctgcgac ttccgataat atcagcgatt caccgccttc caggatttcc
    24481 tggctgctca gcggatttcc ctctccacca atcaacaaac tgatttcacg cggcataaat
    24541 tctttaccgg tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac
    24601 aatgtgcgaa cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct
    24661 tctaacgagg tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa
    24721 tcaagcgcac tatcttcaac cacagcctcg ccctgcgccg ccgtaccagg cacccagacg
    24781 gtggattcca gtagagtacg gaaaaagccc gggcggtgcg ccggttcggt tgctgctttt
    24841 tccagcaggt cttcaagttc gttttttgtt tcggacatat gaaccacaat tcattcaacg
    24901 ttcggggcgc aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg
    24961 ttgaatttgc agaatttgta ggccggataa ggcgtttaca ccgcatccgg catattagtt
    25021 tacgccgtta acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac
    25081 tgttcaaccg gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga
    25141 tagttctcaa caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc
    25201 gcgcttccgg tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc
    25261 gccagcggca gacgccagaa tggttcgttc tcttgtgagg cactcgccag caaacgaccg
    25321 gcaagcgcat cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc
    25381 gccccggtga gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc
    25441 agaccatcgg caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta
    25501 ccgttgcgat aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc
    25561 gcacagcaga ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc
    25621 gccccggtaa ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc
    25681 tgtttgatgc tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac
    25741 accggtgctt ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt
    25801 tctgaaccgc gcccgacggt gtgcagcccc atataacctt gctcacgcag atcttcgcct
    25861 ttggtgatcc gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc
    25921 tgtgccagtt gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag
    25981 tcgatgatca tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac
    26041 tccactttac gcgtgccttt cggggctttg taaccttgcc agaatgccca gcagcgatcc
    26101 gcatcccagc cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgc
    26161 gccgcacgct ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca
    26221 ttattaatgc tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg
    26281 gtaatcttca tcgcttctgt cattttagtt atccttcttt gtaaaagggc cgccagttgg
    26341 cagccccagg atttttccgc taaatgattc gcgttgcagg aaggcggcaa gtgagtgaag
    26401 ccccaggagc atagataact atgtgactgg ggtgaacgag cgcagccaac gcatctgcgg
    26461 cgtgaagcat gacgcggaaa ttattcggcc tcgtccagcc agactaacaa aatcgcttcg
    26521 aggattttct cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac
    26581 tgatgcatat cggtgaatcg aaccgttttc ggatcaagat cgggatacgc atcgtacagt
    26641 gcttcgccaa tttcgcggct atcggtccat ttaagtccca tactaacctc tgttaatgct
    26701 cacgcgcatg gttgatagtg taacgcggga tttcgaccac taaatcttcg tcggtgacgc
    26761 gcgcctggca gcttaaacgg ctttccggct ccagtcccca ggctttgtcc agcatgtcgt
    26821 cttcctgctc tgagctttcc ggcagcgagt caaaaccttc acgaacgatg cagtggcagg
    26881 tggtgcaagc acaggatttt tcacaggcgt gttcaatctc gataccgtta cgcagagctg
    26941 cgtcgagaat ggtttcaccg ctattagctt ccagaacagc gccatcaggg cagagatcct
    27001 gatgaggcaa aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc
    27061 gcacgacgaa ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg
    27121 tttttaatcg cttgttcgat ggcgtcaacg tcatcgccct gcgccacttc actcaggtga
    27181 gcggcagcat cgtcaatgac ctgacgttct gcggcgctta acagcgcggc atcagcagcc
    27241 agcgcgccgt gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcatt
    27301 cgggctttta cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg
    27361 ctatcggtca gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc
    27421 tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc
    27481 gcaccgccag ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag
    27541 tcctgcacca gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct
    27601 ttaaaggtgg tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact
    27661 ttctccacca ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcaac
    27721 agcatttcgc tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata
    27781 gcgacgactt tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc
    27841 cgttcacgca ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg
    27901 tcagcttcta cacccgcgtc tttaagcgcg cgacgacaag ccagtaaggt tcgttttacc
    27961 agtggcgcga tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg
    28021 ttaacggtca cggagtccgc atcgctcagc gcgattttgg ctgcaatggc ggcatccagc
    28081 agttcacgct gaacgcggtt atcgctacga tcaggaatgc ccgcctgctc gcgaatgtaa
    28141 tccgccagca gatggtcgaa atcatcacca ccgagcgcgg aatcaccgcc ggtcgccagc
    28201 acttcaaaca caccgcgact taagcgcaga atggaaatat caaacgtccc gccgccgagg
    28261 tcataaacgg cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagcg
    28321 gtcggttcgt taagtaagcg caggacgtga aggcccgcca gacgcgccgc gtctttggtg
    28381 ccctgacgct gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg
    28441 cctgccaggg cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc
    28501 accgggttca gcagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg
    28561 aattgataag gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg
    28621 cgtttaacag aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa
    28681 cccaccgaat gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca
    28741 tggtcggcta acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg
    28801 cccaggtcaa taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca
    28861 ctaatttgta ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc
    28921 gagttgttcg gcactgctcc gcagtttatc gagaaaacgc agcttacgca cggtatccgc
    28981 cgccgcgtcc cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa
    29041 catctttttc acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat
    29101 ctcgtccagc tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt
    29161 atgctgctcg ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa
    29221 cggatgacgc agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag
    29281 ttgttccgcc tggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg
    29341 aaaacgcagg ctcagcgcct gggtatcgag ttgataacgg gcaggcaagc caaagagggt
    29401 gaagtaatcc ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa
    29461 acaaacccca cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag
    29521 ctttcgccgc aaccacactc atctttaacg ttcgggttgg tgaatttaaa cccttcattc
    29581 aggccttctt ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg
    29641 accacgactt tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca
    29701 aattccagta cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca
    29761 aaccctttgc cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt
    29821 gtaatcgaca taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc
    29881 aatggcggct ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag
    29941 ttcaagttct tcagcaatat cggtattttt gatcgcctgc gcttcgtcga gagacttccc
    30001 tttcacccat tcggtgacca gggagctgga agcgatagca gaaccgcagc cgtaagtttt
    30061 aaaacgcgcg tcttcaatga taccttcatc gttgacttta atctgcaact tcatcacgtc
    30121 gccacaggcc ggtgcaccca ccatgccgct gccgacgttc tcgtcgttgt tgtcaaagga
    30181 acccacgtta cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa
    30241 ttctcctgat tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc
    30301 ctgcttgtac atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac
    30361 taactcgatg gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat
    30421 agagctatgt gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag
    30481 gcttgctgac gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag
    30541 cgactcacct tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag
    30601 gtcaccgttc aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag
    30661 gccgcgcaga cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcctcgcc
    30721 cattccgacg atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc
    30781 gccgtgcatt tgcgcttcga tgcgtacgcg cggtttacga cgtacataca gcgcaccgat
    30841 acctttcggg ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct
    30901 caggtcgata ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg
    30961 agcacggcac atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac
    31021 gtgcatgatg gaaacaagga tggtgtcgtc acgcatcgct gcttcaagtt ctttcaggtc
    31081 gataatgccg ttacgctgcg gtgccaggta ggtgacttca aaaccttcgc gctccagctg
    31141 acggcaggta tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt
    31201 tttctgataa aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga
    31261 ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat
    31321 atctaccgct tcttcagcct gccagccgaa acggtgagaa cgagaggccg ggttaccaaa
    31381 ggttccgtcc atcgtcataa actgcatcat tttctcggca acacgcgggt ccaccggcgt
    31441 ggttgcggag tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac
    31501 tcaatgcaag gaaccaggct accggctgga tgtacgaccg tgtttacgga gcgcgatacg
    31561 ccccggcctg attctaatat cttgttatct ttaacctaac tgtttcgggt ttcagcaagt
    31621 tgacaaactt acgaatctcc gggagcttac ataagtaagt gaccggagtg agtatgtgca
    31681 gtcaacgcag atgcaacacg aaacaggacg gttaaattaa gcgcgtaact taacgtcgat
    31741 cgcgtcttgt atgcgggtgc gtggcgcgtc gtgagtatgc tgacgaccag acacatccag
    31801 cacttcctgg ttattaacca gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg
    31861 gtcgctcaaa tcacgccaca gcgcgtgggt caggcattta tcgccgccct ggcagccgcc
    31921 tttaccctga caacgggtgg catctacaga ttcgtcaacg gcgctaatca cttcgccaac
    31981 ggcgatgctg ctggcatctt tgcctaacag ataaccaccg cctggtccac gtacgctgga
    32041 aaccagacca tttttacgca gacgggaaaa cagttgttcc agataagaaa gggaaattcc
    32101 ctgacgttcg gaaatatcag ccaacggtac cgggcccgct tcagagttga gcgcaacgtc
    32161 aagcattgcg gtcacggcat agcgcccttt agatgtcagt ctcatgtctt acttcacctc
    32221 aaactcgccc ctgcccgggg ttttttattg taaaagtggg ggtattgcat agcagggtca
    32281 agtctgacat tcccgagtaa attggtcaac tatttacttg actgatttag tcgggtattt
    32341 aaccttcagt gccatttttt tatcgtggcg tttgtacctg ttgtccttcc agaccatggg
    32401 cgggttgagg gcaattattc gcattagcgc tggcctcttc ccctcaccct aaccctctcc
    32461 ccgaaggggc gaggggaccg tccgtgcatt tatctggctt agccctcagg ccttattacc
    32521 tttattctgc tgctcaatag aagccagaat cccgcgcagg atattcaatt cctggctttc
    32581 cgggcgcgca cgggtaaaca gacggcgcaa tttattcatc acctgccccg gatggttttc
    32641 acggataaaa ccggttgcca gcagcgtttg ttccagatga ccataaaaac gctccagatc
    32701 atcgaccagc ggatacggcg tctcttcatg ctcgacctgc tcgccgtttt cctgagttgc
    32761 cagccaggcc atgcgcactt cataggcaat aacctgagcc gccatcgcca ggttcagcga
    32821 gctgtattcc gggttagccg caatcgcgac atgataatgg catttctgca actcttcatt
    32881 ggtcaagccg acgcgctcgc gaccaaacac cagcgccacc ggggtatttg ccgcttcagc
    32941 gacgcttttc aggccgcatt cgcgcgggtc gagcatcggc catggcagcg tgcgggagcg
    33001 tgcgctggtg cccaccacca ggctacaacc ggctaacgct tcgtcgagcg tatcgacgat
    33061 gtgagcatta ccaatcacat cgctggcccc tgctgccagg gcaatcgcct gggagtcggg
    33121 tttcaccagt ggattaacca gccacagatt ggttaatccc attgttttca tggcacgggc
    33181 aacagaaccc atattgccgg tgtgtgacgt ctccaccagc acaattcgaa tattttgcag
    33241 cattgtcttt cttcatctaa agattattca cgcatcttat cataaaacga agacagatgc
    33301 cgatctcgct gctatactct gcgccgtttt cccgttcttt aacatccagt gagagagacc
    33361 gatgcatccg atgctgaaca tcgccgtgcg cgcagcgcgc aaggcgggta atttaattgc
    33421 caaaaactat gaaaccccgg acgctgtaga agcgagccag aaaggcagta acgatttcgt
    33481 gaccaacgta gataaagctg ccgaagcggt gattatcgac acgattcgta aatcttaccc
    33541 acagcacacc atcatcaccg aagaaagcgg tgaacttgaa ggtactgatc aggatgttca
    33601 atgggttatc gatccactgg atggtactac caactttatc aaacgtctgc cgcacttcgc
    33661 ggtatctatc gccgttcgta tcaaaggccg caccgaagtt gctgtggtat acgatcctat
    33721 gcgtaacgaa ctgttcaccg ccactcgcgg tcagggcgca cagctgaacg gctaccgtct
    33781 gcgcggtagc accgctcgcg atctcgacgg taccattctg gcgaccggct tcccgttcaa
    33841 agcaaaacag tacgccacta cctacatcaa catcgtcggc aaactgttca acgaatgtgc
    33901 agacttccgt cgtaccggtt ctgcggcgct ggatctggct tacgtcgctg cgggtcgtgt
    33961 tgacggtttc tttgaaatcg gtctgcgtcc gtgggatttc gcggcaggcg agctgctggt
    34021 tcgtgaagcg ggcggcatcg tcagcgactt caccggtggt cataactata tgctgaccgg
    34081 taacatcgtt gctggtaacc cacgtgttgt gaaagccatg ctggcgaaca tgcgtgacga
    34141 gttaagcgac gctctgaagc gttaatgact caggcgggtg atgtcactca cccgccctcg
    34201 cctttcaggc gctattctga aatacttcct caccgcttta ctttctttcg tcactctccc
    34261 accatctttc cccgattaat ggatgataaa gaagtcgcaa ggaaaggtta tggcactgcc
    34321 agtgaacaaa cgcgttccca aaattctgtt tattctcttt gttgttgcct tctgcgttta
    34381 tttagtgccg cgcgttgcca tcaacttctt ctattatcct gacgataaaa tttacggtcc
    34441 cgacccctgg tcggcggaat ccgtcgaatt tacggctaag gacggtactc gtctgcaagg
    34501 ctggtttatt ccttcttcga cgggccctgc tgacaacgcc atcgcaacca tcattcatgc
    34561 tcacggcaat gccggaaata tgtccgccca ctggccgctg gtcagttggt tacccgagcg
    34621 taatttcaac gtttttatgt ttgattatcg cgggtttggt aaatcaaaag gcacgccgtc
    34681 ccaggccgga ttgctggacg atacgcaaag cgccatcaat gtggtgcgcc atcgcagtga
    34741 tgtaaaccca caacggctgg tgctgttcgg gcagagtatt ggcggggcga atattcttgc
    34801 tgttattggt cagggtgatc gtgaaggcat acgtgcggtg atcctcgact ccacatttgc
    34861 ctcttatgca accatcgcca accagatgat ccccggcagt ggctacttac ttgatgagag
    34921 ttacagcggc gaaaattata tcgccagcgt cagcccgatc ccgcttttac tcattcacgg
    34981 taaagctgat cacgttatcc catggcagca cagcgaaaag ttgtatagcc tggcaaaaga
    35041 gccaaaacgg ttgatcctaa tcccggatgg cgaacacatt gatgcttttt ccgatcgcca
    35101 cggcgatgtt tatcgcgaac agatggtgga ctttatcctt agcgcgttga atccgcagaa
    35161 ctaacccatg atcgctagca cgataatcat tcacaaaacc accttaagac atgctaatcc
    35221 actggtcaga acagtttaag atgagaaaaa ttctgtgacg cttgccaaca tttctgatga
    35281 ttagcattcc cttcgccatt tccctgagca aactttagct attcttatca attatgctta
    35341 tgggagatct acagatgatg cctacgcttg ctccaccatc tgtcctttcg gctccccagc
    35401 gccgctgcca gatcttgctg acgctctttc agccggggtt gaccgccacc atggcaacct
    35461 tcagcgagct taatggtgtg gatgatgata ttgccagtct tgatatcagc gaaacaggac
    35521 gggagatcct gcgctatcat caactcacac tgacgactgg ttatgacggt agctaccggg
    35581 ttgaaggtac agtgcttaac caacgtttgt gtttatttca ctggctacga cgtggtttcc
    35641 gtctgtgtcc gtcatttatt accagccaat tcacccccgc cctgaagagt gaactgaagc
    35701 ggcgcggaat tgcgcgtaac ttttacgacg ataccaatct acaagcgtta gtgaatctct
    35761 gctcccgacg gctgcaaaaa cgctttgaat cgcgcgatat tcatttcctg tgtctgtatc
    35821 tgcaatattg tttgctgcag caccacgctg gaattacgcc tcagtttaat ccgctccaac
    35881 gtcgctgggc ggaatcctgc cttgaatttc aggtagcgca ggaaattgga cgccactggc
    35941 agcgtcgggc gctccagcct gtaccgcctg atgagccact gtttatggca ctactttttt
    36001 ccatgttgcg ggttcccgat ccactgcggg atgcgcatca gcgagacaga caattgcgtc
    36061 agtctatcaa acgtctggta aaccattttc gtgagctggg aaatgttcgt ttttatgatg
    36121 aacaggggtt atgtgatcag ctttataccc acctcgccca ggcgttaaat cgcagtttgt
    36181 ttgccatcgg tattgataat accctgccgg aagagttcgc cagactgtac ccacgcctgg
    36241 tgcgcaccac ccgcgcggcg ctggccggat ttgaaagtga atacggcgtc catctttctg
    36301 atgaggaaag tggtctggtc gcggtgattt tcggtgcctg gctaatgcag gaaaacgatc
    36361 tgcatgaaaa acagattatt ctactgaccg ggaatgatag cgagcgagaa gcgcagattg
    36421 agcagcagtt acgcgaacta acgttactgc cgctcaacat taagcatatg tcggtaaagg
    36481 catttttgca gacaggcgct ccgcgtggcg cggcactgat tattgcgcct tataccatgc
    36541 cattaccgct cttttcacca ccgctgatct atacggacct gacgctgaca acacatcaac
    36601 aggagcagat ccgcaaaatg ctcgaatcag catgagggaa caactttcgg gcgcaaaaac
    36661 attgccggca gcgccaccag cgccattacc cagaacacgc cgtggcccag atattgatac
    36721 aggaaaccgg cgaaaacggt catgatagcg atactgccgc ccatcgcgac ggcagagtaa
    36781 accgcctgta aacggatgac ttcgctaccc tggcgagcag caatataacg catggcggcc
    36841 aggtggcaga ccgtgaaggt gccgcaatgc agaatttgca ccactatcaa ccacggcaac
    36901 gccgtagttg ctcccataat gccccagcgc actacgccgc aaatcgccga gatcaacagc
    36961 atatcgcgtg cactacaacg gcggaaaagt ttattactca gcgcaaagat aatgacttcc
    37021 gccaccacgc ccagcgacca caaatacccc accgccgagg ccgagtagcc agctgcctgc
    37081 cagtaaatgg cgctaaaacc gtaataggcc gcatgtgccc cctgcaataa acaaacgcag
    37141 gccagaaagc gccagttctg gcgaaccagc gccaaccacg cagaccaacc ggtgctctcc
    37201 tgctggcgac ttgccccttg tggctgaatc gtcggacgga tgagaaagcc gagcagcatg
    37261 gatgccacgc ccaacgtcaa cagcgcgagg atcacccgat aatcaaacat agtgaccagt
    37321 ttgcccgtca gcgccgagcc aatgacaaac gccaccgagc cccacagtcg cactttgccg
    37381 taatcaagcg ggaactgctt ttgccacgta ttcgccagtg catcggtcaa cggtaccagt
    37441 ggtgagaaaa agaggttaaa gccaatcatc accagcatca gccacgctac gtgcgccccc
    37501 gcccagaagg cgacagcaaa gagaagtgtc agcagtgcca gcacgcgcaa ggcggaaatc
    37561 aggcgggaag gatcgctgac gcggggcgcg atgagcaaac tcccgaggaa acgggcaacc
    37621 agacctgccc ccaataacag gccgatggtt tctggcgtta aaccaatccc tttaagccag
    37681 acgctccaga aaggtagaaa aatgccgtaa ctaaaaaagt atgtgaaata gccgagcgcc
    37741 aaccagcgcg tggattgcaa aaccatgagt ccctcccgtc aggaggcgtt agtctggggt
    37801 taatcgaatg ttggtgcaag ttgaaaaata agaatattcg agcctgcttc ccgatgtctg
    37861 taacgccagt tcacaagcac taaaaaactg agatccgcga cgcatgttgc aaaaataatg
    37921 cgtaattatg ccgttacgct tgccaaacgt tcctgcacaa tggcgatgaa atcgcgtaat
    37981 gccggcttca tctccccttt cttccacgcc atcagtaaag caatggaagg cacatttccg
    38041 gcaatgggac gaaaaacaac ctgtccggta ttaaaattat tcatataacc gggtatcaaa
    38101 gtgacgccca gccccatgcc caccagattc atggtcacca gaatattcgt tgccacctgg
    38161 acgatatttg gctggctgtt ttcttgcgca aaccacgctt taacgatcgg cgcaagcgaa
    38221 ccggaataca ccggatcggt actgacgaaa ttcacgccat ccagttgcgc ggcggtgatc
    38281 tctttttcat gtgctaaagg gtgatcaacc ggtaacacaa ccactaatgg ttcgtcaaaa
    38341 agctccagat aatcaatctc cgggctataa acaggatggc gcattaagcc gacgtcgagt
    38401 tcaccacggc gaattttttc ctcctgttgc gtggtgatta aactcaccag ctcaatcaag
    38461 gtgtctggct gtctgagacg aaacatcggt aatacttttg gcagtaaatt cacttccgcc
    38521 gatggcacaa agccaatggt taattgtctg tcttcctgaa caattttccg cgcccgtaat
    38581 ttggcatttt ccgcttgttc gaggattgcc agcgcgtcct ggagaaaaca ttctcccgcc
    38641 gccgtcaacg cgactttgcg cttatccctc accagtaacg gaacaccgac acagttttca
    38701 agatcgcgga tctggctgct taacgaaggc tgtgaggtat gcagtttttc cgccgcacgg
    38761 gtaaagttca gtgcctgcgc cactgcgacg aaatagcgta aatgccgtag ttccatcacc
    38821 ttccccttgt tatcgaaaaa acgtctcagc tggtagaaat taactatttc acatattagc
    38881 aaccaaccag caacatcctt atggcacaaa aatagaaggt caatacatct tatctttcag
    38941 gattaaaaaa tatgaccaca ccctcagatt tgaacattta ccaactgatt gatacccaaa
    39001 atggtcgggt cactccgcgt atttataccg acccagatat ttaccaactg gagcttgaac
    39061 gtattttcgg ccgttgctgg ttatttctcg cccacgaaag ccagatccca aaacctggtg
    39121 atttctttaa cacctacatg ggggaagatg cggttgtcgt agtgcgtcag aaagacggca
    39181 gcattaaggc gtttctcaac caatgccgcc accgggccat gcgtgtgagt tatgcagatt
    39241 gcggcaacac tcgcgccttt acctgtccgt atcacggctg gtcttatggc attaacggcg
    39301 agttgatcga tgtaccgctg gaacctcgcg cctacccaca agggttgtgt aaatcccact
    39361 ggggactaaa cgaagttcct tgtgtggaga gttataaagg gctgattttt ggcaactggg
    39421 ataccagcgc accgggcctg cgtgattacc tgggtgacat tgcctggtat ctggatggca
    39481 tgctggatcg tcgcgaaggc ggcaccgaaa ttgtcggcgg cgtacaaaag tgggtgatca
    39541 actgtaactg gaaattcccg gcagagcagt tcgccagtga ccagtatcat gctctgttca
    39601 gccatgcttc tgccgttcag gtattagggg cgaaagatga tggcagcgat aagcgcctcg
    39661 gtgatggaca aaccgcccgc ccggtgtggg aaaccgccaa agatgcgctg caatttggtc
    39721 aggacggtca cggtagcggt ttcttcttta ctgaaaaacc ggatgctaat gtctgggtcg
    39781 atggcgcagt ttcaagctat taccgcgaaa cctatgccga agcagaacaa cgtttaggtg
    39841 aagttcgcgc cctgcgcctg gcgggtcata acaatatatt ccccacgctt tcatggctaa
    39901 acggcactgc cacactccgc gtctggcatc cgcgcggccc tgatcaagtt gaagtgtggg
    39961 cgttctgtat tactgacaaa gccgcctccg atgaagttaa agccgctttt gaaaacagcg
    40021 ccactcgtgc ttttggtcct gctggttttc tcgagcagga tgactcggag aactggtgtg
    40081 aaatccagaa attgcttaaa ggccaccgcg cccgcaacag caaactgtgt ctggaaatgg
    40141 ggcttggtca ggaaaagcgc cgcgacgacg gcattcctgg cattactaac tatatctttt
    40201 cagaaactgc cgctcgtgga atgtaccaac gctgggctga tcttctgagt agcgaaagct
    40261 ggcaggaagt gctcgataaa accgccgctt accagcagga ggtgatgaaa tgagtgcgca
    40321 agtttcacta gagttacatc accgcattag ccagtttctc tttcacgaag ccagcttact
    40381 ggacgactgg aaatttcgtg actggctggc gcagctcgac gaagagattc gttacaccat
    40441 gcgcaccaca gttaacgcgc aaacacgcga ccgccgcaaa ggcgtccagc caccgacaac
    40501 ctggattttt aatgacacca aagaccagct ggagcggcga atcgcccgtc tggaaacggg
    40561 catggcctgg gcagaagagc cgccgtcacg cacccgtcac ttaatcagca actgccagat
    40621 aagcgaaacc gacatcccaa acgtatttgc tgtgcgggta aattatctgc tttatcgggc
    40681 acaaaaagag cgcgatgaaa cattctatgt tggaacgcgt ttcgacaaag ttcgccgtct
    40741 ggaagatgac aactggcgct tgctggaacg ggatatcgtc ctggatcaag cggtaatcac
    40801 ttcccataac ctgagtgtac tgttctgatg aatcgaattt atgcgtgtcc cgttgcggat
    40861 gtgccggagg gtgaggctct ccggatcgat acctcgcccg tcatcgccct gttcaacgtt
    40921 ggcggcgagt tttatgccat taacgatcgt tgcagccatg gtaatgcgtc aatgtcagaa
    40981 gggtatctgg aagatgacgc cacggtggag tgcccgctac acgccgccag tttttgcctg
    41041 aaaacgggga aagcgttatg cctgcccgcc accgatccgc tcaccactta tccagtacac
    41101 gttgaaggtg gtgacatttt catcgactta ccggaggcgc agccatgagc gatctgcata
    41161 acgagtccat ttttattacc ggcggcggat cgggattagg gctggcgctg gtcgagcgat
    41221 ttatcgaaga aggcgcgcag gttgccacgc tggaactgtc ggcggcaaaa gtcgccagtc
    41281 tgcgtcagcg atttggcgaa catattctgg cggtggaagg taacgtgacc tgttatgccg
    41341 attatcaacg cgcggtcgat cagatcctga ctcgttccgg caagctggat tgttttatcg
    41401 gcaatgcagg catctgggat cacaatgcct cactggttaa tactcccgca gagacgctcg
    41461 aaaccggctt ccacgagctg tttaacgtca atgttctcgg ttacctgctg ggcgcaaaag
    41521 cctgcgctcc ggcgttaatc gccagtgaag gcagcatgat tttcacactg tcaaatgccg
    41581 cctggtatcc tggcggcggt ggcccgctgt acaccgccag taaacatgcc gcaaccggac
    41641 ttattcgcca actggcttat gaactggcac cgaaagtgcg ggtgaatggc gtcggcccgt
    41701 gtggtatggc cagcgacctg cgcggcccac aggcgctcgg gcaaagtgaa acctcgataa
    41761 tgcagtctct gacgccggag aaaattgccg ccattttacc gctgcaattt ttcccgcaac
    41821 cggcggattt tacggggccg tatgtgatgt tggcatcgcg gcgcaataat cgcgcattaa
    41881 gcggtgtgat gatcaacgct gatgcgggtt tagcgattcg cggcattcgc cacgtagcgg
    41941 ctgggctgga tctttaagga agcacgatga aagaaaaaac catcattatt gtcggtggcg
    42001 ggcaagcggc ggcaatggct gcggcctcgc tacgccagca agggttcacc ggtgagctgc
    42061 atctgttttc cgatgagcga catcttcctt atgaacgacc tccactctcg aaatccatgt
    42121 tgctggaaga ttccccgcag ttacagcagg tgttacccgc taactggtgg caggaaaaca
    42181 atgttcatct gcattccggt gtaaccatca aaacgctggg tcgcgacaca cgagagttag
    42241 tgttaaccaa cggcgaaagc tggcactggg atcagctttt tatagcaacc ggcgcggcag
    42301 ctcgaccgct gccgttgctt gatgcactgg gagaacgctg ctttacccta cgccatgccg
    42361 gtgatgccgc cagactgcga gaagttctgc agcccgaacg gtcagtcgtg attgtcggtg
    42421 ccggaactat tggtctggaa ctggctgcca gcgccacgca gcgcagatgt aaggtgacag
    42481 tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc accgcccgtg caacgctatc
    42541 ttttacagcg ccaccagcag gctggtgtgc gcattctgct caataatgcc attgaacatg
    42601 tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg ggaaacgctt caggctgatg
    42661 tggtgattta cggtattggt atcagcgcca acgagcaact ggctcgcgag gccaaccttg
    42721 atactgccaa tggcattgtc attgatgagg cttgccgcac ctgcgatccc gcgatctttg
    42781 ccggtggcga tgtggcaatc actcgtcttg ataatggtgc actacaccgc tgcgaaagct
    42841 gggaaaacgc caataaccag gcgcaaattg ccgctgccgc aatgttaggg ctaccgctac
    42901 cgctactgcc gccgccgtgg ttctggagcg atcagtacag tgataactta cagtttattg
    42961 gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc ggaaactcag aaggcgattt
    43021 ggtttaatct gcaaaacggc gtgcttatcg gtgcggtcac gctgaatcag gggcgtgaga
    43081 ttcgcccaat tcgcaaatgg atccagagcg gcaaaacgtt tgatgcgaaa ctgctgatag
    43141 atgagaacat cgcgcttaaa tcactgtaac caggataatt agcgaatatc tcaatgcctg
    43201 gggcgtggcg aggtgcaaga gtgtgtatta cgtttaaatc acattatctt gcaaagggat
    43261 tggttatgaa cacactacgt tattttgatt ttggagctgc ccgccccgtt ttgttattaa
    43321 ttgcccgtat cgccgtggtc ttaattttca ttatttttgg ttttcccaaa atgatgggct
    43381 ttgacggtac ggtccaatat atggcctcgt tgggcgcgcc aatgccgatg ctggcagcga
    43441 ttattgcggt agttatggaa gtgcccgccg cgatattaat cgtgcttggc tttttcaccc
    43501 gtccgctggc ggtgctgttt attttctaca cgctgggtac ggcggtgatt ggtcaccatt
    43561 actgggatat gaccggcgat gcggttgggc caaatatgat taatttctgg aagaatgtca
    43621 gtatcgctgg cgcattcctg ctgctggcaa ttaccgggcc gggggcaatt tctctcgatc
    43681 ggcgttagga aagatgccgg atgcggcgtg aacgccttat ccggcattta ataaattaca
    43741 gccactctac ccgcaacgac atctctgaag ttgttgattc ccctggtgct agcgcaatga
    43801 gatcaccccc ttccgggcga tgatgatcat ccggcgcatg gctcatcggt tccagacaga
    43861 agaaatcaaa cgcatatcct ttatcaaacg cagggtcgga aacaaagatg aaataacacg
    43921 gtgcaggtgg cgtcgtttcc atgatgatgg cataaccttc ctgcggctgc tcgatgcggg
    43981 cctgaccatt ccatccggca aaaccattgt tcacccactg gcgcggcaac ggcgcgggct
    44041 ggttaaaatc cagttcctgc ggtagctgct cgcaaaactc acccgccagc cactgctccc
    44101 gctccagcca gtaaccgctc gcctgtgcct gaatccgcgt ttgcggcgac aacggaaaat
    44161 aaggatgcca gccggtacca aatggcagcg tctctgcccc ttgattggtg acagagagcg
    44221 tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact tacccgatag tgatagacac
    44281 cgctgcgatg ttcatacacc agacacaaac tatcatcgct gtgcgagaca cattgccact
    44341 cgcccagcca gccatcgccg tgtagatagt gcgcatccca ctcaacattc ggttgcagtt
    44401 gatactcacg cccctgccag acaaaacgat tgccgctcac ccggtttgca aatggcacca
    44461 gcggaaaaca tgaggcatcg gttgccacac cgcttttttt accaggacgt aagagcggcg
    44521 tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc agaaacgtcc agctttagcg
    44581 acccgtggga taaggtatag atggtcatcc gctaactcct taatccggga agttaatcac
    44641 aactttcccg catttgccgc tcgccatcag cgcataggca tcacctgcct gttccagcga
    44701 gaagcgatgg gtaatggcgt tacgcggcca cagtttccag tccgtcagat catgggcgca
    44761 tttttccata tggaacagac tggtcaccca ggagccgata atccgccgtt gatggtgcat
    44821 cagatcggcg ctgacctcga actccacttt tccggtttct ccgatgtaaa ccacccgtcc
    44881 ccagtcagcg gtggatcgca gtgccagcaa gcgacctgcg gcattaccag aacaatcgag
    44941 cgcaacatcc gcgccaccgt gggtgagttc ggcgataatc tgcggcagac cttcggtggt
    45001 tgctaaatag ccgtgatcca tcacccctaa ctgttttgcc atcgccagac gttccggcag
    45061 catatcaacg ccgatgatcc gttttgcacc gcgacctttc gccagcatca tcgccatcat
    45121 gccgactggc cccagaccga ccaccagcac gttatcactg ccggaaactt cgccgcgcaa
    45181 aattccttca tacgctgtac caacgccgca actgataaac gcaccatctt cgtagctcag
    45241 cgcatccggc aggaggatca gatctttttc ttccgccagc agatattcgg catgaccgcc
    45301 gtcacgctgc cagccgtaag ccgcttttcc tttgccagta caagagatag gaaagccgcg
    45361 gcggcagttc gggcaaaaac cacagccaga aatgtgatac accagcacgc ggtcgccctc
    45421 tttaaaatgg cggcagcctt gccccatcgc cacaatctgc ccgcacggtt catgaccgtt
    45481 gataaatccc tggtataacg gtttatcggg tgccgccgcc gtggcacggt gttgatgata
    45541 gatatagtgg acatcgcttc cgcaaatccc ggaggatttc attttgatca gtacctggtt
    45601 aatccccggc gtcggcaccg caacttcccg cagatcgacg gttgaatttc ctggtaaata
    45661 agctgccagc atcgttttca taaatccctc attaacaata cgattaattt tcatccctgc
    45721 cgcacccgcg ccaggggcgt taaggtttag cgtttcgctt tactgcttcg ctgggtcagc
    45781 aagatattcg ccagcaccgc caccacgatg atgacgccgc gtaccacctg ctggaaaaag
    45841 gagttaatac cgagcagcac cagaccgtta ccgattagcg taatcaccag cacaccaagc
    45901 aatgtaccga acagggaacc gcgaccgccg gaaagtgccg taccgccgac cacgaccgcg
    45961 gcgatgacgt caaactccag accgtttgcg gcacctgcgt taccggaacc gaggcgtgcc
    46021 gccagcaaaa tgccggtcac cgccgctaat aatcccgaaa gggtaaagat aagaatgcgc
    46081 actcgacgaa cgttaatacc gcacaactgc gccgccgtgg cattaccgcc aacggcaaaa
    46141 accgagcgcc cgaaggcggt ttttcggcta atgaacacaa acagcgcaaa caacaccatc
    46201 atgatcagcg cggatactgg cacaccgaga aattgtccgc ccagccagtc cagcacctcg
    46261 ttttcgtcaa tcggcactgg cagtgcgttg gtcataaaca gccccattcc gcgcagggcg
    46321 ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa acacgccgcg cagcaccccg
    46381 gcgagcgttc ccatcagcgc gcctaacagc aacaccagca gacacgccac cgccagcgga
    46441 acttcaaatt gcagcaaaaa tgccaggcac accgagacaa aagccaccat cggcccaacg
    46501 ctgacatcaa tttcaccgga gataataatc agcgtcatcg cccaggcggc aatcccaatg
    46561 gtggcggcat cgcgcagcac gttcatctgg ttattcaatg agataaagcc tggcgcgttc
    46621 agggagaaga ccagataaag aatggcaatc accaccagca aaccgatctc attaatatgg
    46681 cgactgacaa attgtttgag cgagacgctc ttgccctgcg gcaatggtaa tgacgaagca
    46741 gacatcttca ggttcctcgg taaaattcat cagtgcacag acagaatggc ggacatcagc
    46801 tcatccacat tgaccggagc gtgaaactcc tgcgagaacg tgccgtgctg tagtaacagg
    46861 atgcggtcac agaccagcgg caactcctcc acttcactgg agataaacac cacgcttttt
    46921 ccttcggcag ccagctcacg gacaatacgg taaatctgct gtttggcttc gatatcgacg
    46981 ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg catagaccca acgaccgatc
    47041 accacttttt gctgattgcc gccagaaagc gtgccgatgg gcgtttcgct actggcggcc
    47101 ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc ggatggtgga ccattgcagt
    47161 acgccgttgg cgctgatttt ttgccgattg gtcagcactg tattttcgtc aacgcccaac
    47221 cagggaatga tccccgcttc tttgcggttt tctggcgtat agccaatgcc gcgtttcagc
    47281 atgtcgccgt aatcggggcg cgtgattttc tcgccgttga taacaatttc gccctgttca
    47341 tactcctcca gcccaacaat cgccttcagc aattcactgc gccctgcccc cagcaggcca
    47401 gcaatgccaa gcacttcgcc gcgacgtaac gtaaagctga tatcctccag cttgggctta
    47461 tggcgtaatg cacggacttc cagcacggcc tgatccacaa tttcctgagg ggcaaccggc
    47521 gcaatatcaa cgtgatcacg ccctagcatt agcgacacaa tatgatgcgt ggaggtgttt
    47581 tcgagcatca catcgcccgc cacctgacca tcgcgcataa cggtggcaca ggaggcaatg
    47641 cggcgaattt cttccatccg gtggctgaca taaatcaccg ccacgcccag tgccgacatc
    47701 tttttcaccg cgatgatcac cagttcaact tccgcactcg caagcgaact ggtaggttca
    47761 tcaagaatga ccacgcgcgg ctcgcccttc atcacccgcg caatttccac cagttgcttt
    47821 tgcgccgggc ttagcgttga aacaagttgt tcagggctaa cgtcaacgcc cagcgcctgt
    47881 aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa tcatgccgtt gcggcggggc
    47941 cactgaccga ggcagaggtt ttccgccacc gtcagtccct ccaccagact taactcctga
    48001 taaaccgcac gaacccccag ttcagcggca cggcgagtca acgtagcttc gtcaccttcc
    48061 agtcgcgtct cgccaatcca gatatcaccg ctatccgggc gttcgctgcc ggtaagcatt
    48121 cgaatgagag tcgatttgcc cgcgccgttt ttacctaaca gcgcacgaac ttcgccttta
    48181 ttgagcgtga agttaacgtt atccagcgca acgacgccgg ggtaacgctt atttcctgcc
    48241 accacttttg ctaccgggac tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag
    48301 cgattagggc agaccatcaa cgtgcgttgc cagccactgt ttaccatctt ccgttttggt
    48361 atagagatcg attggcacct gaatcacttt ttcaccgtcg gcttgtttat tgataacctt
    48421 caatgtttgc gcgaaaacag cattgcccat tttcttaccg gaaatatcca ctaccgcttt
    48481 cagcacctga ttgttttcca gctcctgagc aatttcggtt gtcatatccg aaccgaaaac
    48541 agcaattttt ccggcctgat tttgattacg taccgctttt accgcgccga gtgtcgcacc
    48601 gcccgactcc cccataatgg cgttgagatc cggcgtggag ataatcagtt tttcaccaac
    48661 ggaaatcgct ttatctaaaa cagtcccttc ctgattagcg acaatttgcg cgccgggaac
    48721 gcgggatttt aatacttctt caaatccttt acgtcgctgc acacaaactt caaaggcttc
    48781 gcaattgatg acagcaattt tcggctggtc aattttattg gcaataaaat aatcggcggc
    48841 agcgttaccc agttttttac caaattccag cggatcgccg accagatacg ccgagacata
    48901 tttatcgccc cccttttgat taatacaggt gttgtagcaa atcaccggaa tacccgcttc
    48961 actggcgcga cgaacggtac ggctactgcc attttcagac actgccgata aaataatggc
    49021 atcgacatta cgcgccacga gggtatcaac aaaggtactt tctttcgaaa tatcaccctg
    49081 ggcgttagtt tcaattaact gtacctgtac tgaagaatct tttgccgcat cctgaacgcc
    49141 ctgacgcact ccagcgtaat atccctgggt atcaaggtat attgcaccaa tggtcatttc
    49201 tttatccgca gccctggcaa atagtgcgct acctaatagc gtagccatta ataatagatt
    49261 acgggttgtt ctcatttttt taggcatagt tcctcctgta gggtttttat taacaacggc
    49321 ttattctaat tattttgtga tgagcggcag cgcgtgccgc tcaaatatta cagttaatag
    49381 ggtttagtga acgtagttaa aaataaaagg catcacggtg gtgaataatg ccgctttcgg
    49441 ccaggccgga ttaatcgccg tcaattcagc gcgagcagat tcatattgcg caacctcccc
    49501 gagtcccgcg gacgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa
    49561 atcgccatac agcgacaaca ggtcaggttg tgaaacagca aagaaatccg cctcaatact
    49621 ggttttcgcc atctcttgcg cccactgttt catttcacta aacagttgct gtgcggtttg
    49681 ctgttcaccc agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc
    49741 gttgtaataa ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg
    49801 catcgcttca gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc
    49861 gttatcagtt tgccccggta aacggccttc gcttaaattc tccggataat gcagcgcggc
    49921 atgaagcagt tcgctggcct gctgcggctc tctggcatca agatgctgcc aggcgcgtaa
    49981 taactggttg aggataaact gactggtgac cttcccttcc ccgccttccc acgggtggaa
    50041 tttacgcgtg gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat gccacaaatt
    50101 gagcagttct gcggtcatgt cgtcgcgttt cagcgcaatt tcctgattat tttccagacg
    50161 cgccagtcgt ttctccggtg tggctccact taacttatca agcaaatccc gttcgaaaag
    50221 cagacgtgca tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc
    50281 ataatcgtgt tgcttattcc acgcatggat cgctaacccg cgccagccgt cggcaaactc
    50341 cggcgacatc tctacgcaac gttgccataa ggcaatggct ttgttgtagc tacgtttgtt
    50401 gtagtagaag caggccagta aatggcgagc aaaccagcac tcttcaatac tctccagcgc
    50461 cgccacttct tccagcgtat tcgggaaacg gacaaactgc gggaagacat caatggcttt
    50521 tgcgaccagt tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt aaagcggcag
    50581 cgtgcgctgg cagtccagtg cgttcagcat ctctgccgcc agggtgggca ttccccagtt
    50641 aatcagttgc ccggcggtca tcagggcgtt aacgtcgcgt ccctgacaca gaccgcgcca
    50701 ctgcgcgaga gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt
    50761 cagcggataa tcgcgcagca gtttctcgcg ctgcacacgc gcgttgtcct gacgaccact
    50821 taacaccagc agcagattat gcaggcaaag cacttcctga ttggttgggc aggcgcgaag
    50881 actttgttgg caaaaatcca gaccagcgtc gaagttacca ttacgcgccg ccagtcgtgc
    50941 caggccataa tagccaccgg ctttgctgtt gccgctccag accgcacgcc agaaatcctc
    51001 ttcggcttgt tgatattgtc cctgacgttc gtaagcactg gcgcgaatca aactcgcctg
    51061 tccgcactgc ggatttttgt tcagcgcatg tgcgcgtttc agagcctgag tggcatacgc
    51121 caccgcttgc gggaaatcgg cgcggttata ttccagcatc gccagcgcca ggttacagcg
    51181 gtaatccagc ggatccagcg ccacgccgcg caggtagtaa tcgaacggtg aacggctggc
    51241 gtgatgatat tgctccagat gctgaccgat aaaccaggct tcatctgtac tggtaatgtc
    51301 ttgtgctgcc agtggcgctt tggcgacgtc cggcaacggc aactcttgcg gctgatgttc
    51361 ctgataactc agtacaatat tgccgtcggc atcggagagc tcaatggtca gcctttcagg
    51421 attgataccg tgcaacacgc cctggatggc ggtcgcaggc atcagtgcca cggcatcatc
    51481 aagtaacgcg ttgcatttgc cgatttcgcg gatcgccagg cggtatccgt tcaacggaga
    51541 gatggcatac agcccccact caatcccccg cttactacgc tggagtttta tcaccgcatc
    51601 gcgggaggca ttttgcacca tgcccaaaga atgataaggc aggaaatact gctcaaaacg
    51661 cttctcttcg taagcatcaa gccaggtaaa atcgggctgg ttatcggcaa aaataccggt
    51721 catcagttcg atatacggac cgttattgtc ggtcagactc ttatcccacg cctggccaaa
    51781 ttcactgtgt ccccaactcc actgtttttt acctggcgca atatggtggt tggcaacgtg
    51841 cagcaaaccg ccatcttcat cgtgacacca cgcgccgaca aaatcgtact gcgatttttc
    51901 ggccatatat gaggttggaa caggcacatt tttatagcga gaaatgtcca ctccggcgga
    51961 gtagtccact ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttgcc
    52021 gtgatcaaac accgcagtca catccggtgg gaaaacgctc tgatgccctt cccccccttt
    52081 cactgccggg ttggcccacc acaagaaatg acgcggcgtg gcgttaccgt tatagacgcg
    52141 gctggcgatt tccagcgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa
    52201 accatgcatc ggctccgttt cgcctaccca caccgtctgt gcaccgtctt catgggcttc
    52261 gagggtgaaa tcaacgggca taaaggtggt cgggcgatga tgttgcggcc agttaaactc
    52321 aatcccaccg gaaatccacg gccccagcag ccccaccagc gcaggtttaa tgacttcatt
    52381 gtgataaaca aaatcgcgct gtttcacttt atcccacgcg cgatgcaccc gaccgcccag
    52441 ttccggcagg atcatcactt tgatgtagtc gttttccagc cacaccgcct gccaggattt
    52501 cagggttttc tgctcgctca gcgtatcggt cacgccgtag ggataaaccg cgccggacga
    52561 tccctgataa acgcgatttt cgaggaacat gggatgtata tcctgcggcc cggtttcata
    52621 ggtcgggatc tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt
    52681 aacaagacat actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt
    52741 cgcggaataa tcacgcaatt aactaaacaa ggtttagtga agatgagagc ctgcattaac
    52801 aatcaacaga ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag
    52861 cgcgccaata aatcaacgct ggcccggctg gcgcaaattt cgattccggc agtcagtaat
    52921 attttgcagg aactggaaag cgaaaaacgg gtggtgaata ttgacgatga aagccagacg
    52981 cgcgggcata gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgcctg
    53041 aacgtgacgc ccaccagtat tgagtgtcag gttgctaatg cttgtttaag tccgaaaggt
    53101 gaatttgagt atttacagat tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa
    53161 aaatgctggc atcgccaccg taaattgtgg ccggaccgca ccatcaatct ggcgctggca
    53221 atccacggtc aggttgatcc tgtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg
    53281 acaacgccgg tggaggtgaa atatctgctg gaagagaagc tcggcattcg ggtgatggtc
    53341 gataatgact gcgtgatgct ggcgctggcg gagaaatggc aaaataattc gcaggaacgg
    53401 gatttctgcg tgatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa
    53461 atttatcgcg gcagtttgta tggtagcgga cagattggtc acaccatcgt taatccggat
    53521 ggcgtcgtct gcgactgtgg acgttatggc tgcctggaaa ccgtcgcctc gttaagcgca
    53581 ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg ttagtactca acttgatcct
    53641 gaaaaactga ctacagcgca gttaatcgct gcctggcaaa gtggagaacc gtggatcacc
    53701 agctgggttg accgcagtgc caatgccatt ggtttgagtc tgtataactt cctcaacatc
    53761 ctcaatatta atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt
    53821 aatactatta ttcgccagac aggatttaac ccgttcgacc gcgacgaagg accgagcgtg
    53881 aaagcgacgc aaattggctt cgggcaatta agccgcgcac aacaggtgct gggaattggc
    53941 tatttgtatg ttgaggcgca gttacggcag atttgatggc gcaataacgt agaaaggctt
    54001 cccgaaggaa gccttttttc aacagattaa ccgtttcgct tatgcgtaaa ccggataacg
    54061 tgcgcagatg tcgagaactt tacctttgat gcgctcgata acagcttcat cattgatgct
    54121 gtccagcacg tcacacatcc agccagccag ttctttcgct tctgcttctt tgaagccgcg
    54181 acgcgtaatt gccggagtac ccacgcgaat accggaggtc acaaacgggc tcttcggatc
    54241 gttcggtacg ctgtttttgt tgacggtgat gttagcacgg cccagagcgg cgtctgcttc
    54301 tttaccggtc aggtttttat caaccagatc aaccaggaac aggtggttat cagtgccgcc
    54361 ggaaaccact ttgtagccgc gctcgaggaa cacttctacc atcgctttag cgtttttcgc
    54421 gacctgctgc tggtaagttt tgaactcagg ctccatcgct tctttcagag caaccgcttt
    54481 accggcgatt acgtgcatca acggaccgcc ctgaccacca gggaaaacgg cagagttcag
    54541 ttttttgtac agctcttcgc taccaccttt cgccaggatc aggccgccgc gcggacccgc
    54601 cagggttttg tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt tcgggtagac
    54661 gccagcagca accaggcccg caacgtgcgc catatcaacg aacaggtaag caccgatgct
    54721 gtcagcgatt tcacgcattt tcgcccagtc caccacgccg gaatatgcag agaaaccacc
    54781 gataatcatt ttcggcttgt gttctttggc ttgtttttcc agatcggcgt agtcgatatg
    54841 accggtagca tcgataccgt aaggaacgat gttgtacagt ttaccggaga agttaaccgg
    54901 agaaccgtga gtcaggtgac cgccatgcgc caggttcata cccagaacgg tatcacctgg
    54961 ttccagcagc gcggtgtaga ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt
    55021 agcgtagtca gcgccgaaca gttctttcgc acgatcgatc gccagttgtt caacgatatc
    55081 aacatactcg caaccgccgt agtagcgttt gcccggataa ccttcagcat atttgttggt
    55141 cagctgagaa ccctgcgcct gcattacgcg cgggctggtg tagttttcgg aggcgatcag
    55201 ttcgatgtgc tcttcctgac gtactttttc ctgctccata gcctgccaca gttcggcatc
    55261 ataatcggca atgttcattt cacgctttaa catccgcatc tcctgactca gctaacaata
    55321 aaatttttgg cctttatagg cggtcctgtt ggacaacggc gaacagtata accgaatcat
    55381 tgtgcgataa caggtcttga caaaggaatt tacgcaaacg attaccttca ggctacgcaa
    55441 ggctttggag aataaagagc ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca
    55501 aatttttcac ttcatcacat tctttctgaa aaacaccaaa gaaccattta cattgcaggg
    55561 ctatttttta taagatgcat ttgagataca tcaattaaga tgcaaaaaaa ggaagaccat
    55621 atgcttgacg ctcaaaccat cgctacagta aaagccacca tccctttact ggtggaaacg
    55681 gggccaaagt taaccgccca tttctacgac cgtatgttta ctcataaccc agaactcaaa
    55741 gaaattttta acatgagtaa ccagcgtaat ggcgatcaac gtgaagccct gtttaacgct
    55801 attgccgcct acgccagtaa tattgaaaac ctgcctgcgc tgctgccagc ggtagaaaaa
    55861 atcgcgcaga agcacaccag cttccagatc aaaccggaac agtacaacat cgtcggtgaa
    55921 cacctgttgg caacgctgga cgaaatgttc agcccggggc aggaagtgct ggacgcgtgg
    55981 ggtaaagcct atggtgtact ggctaatgta tttatcaatc gcgaggcgga aatctataac
    56041 gaaaacgcca gcaaagccgg tggttgggaa ggtactcgcg atttccgcat tgtggctaaa
    56101 acaccgcgca gcgcgcttat caccagcttc gaactggagc cggtcgacgg tggcgcagtg
    56161 gcagaatacc gtccggggca atatctcggc gtctggctga agccggaagg tttcccgcat
    56221 caggaaattc gtcagtactc tttgactcgc aaaccggatg gcaaaggcta tcgtattgcg
    56281 gtgaaacgcg aagagggtgg gcaggtatcc aactggttgc acaatcacgc caatgttggc
    56341 gatgtcgtga aactggtcgc tccggcaggt gatttcttta tggctgtcgc agatgacaca
    56401 ccagtgacgt taatctctgc cggtgttggt caaacgccaa tgctggcaat gctcgacacg
    56461 ctggcaaaag caggccacac agcacaagtg aactggttcc atgcggcaga aaatggcgat
    56521 gttcacgcct ttgctgatga agttaaggaa ctggggcagt cactgccgcg ctttaccgcg
    56581 cacacctggt atcgtcagcc gagcgaagcc gatcgcgcta aaggtcagtt tgatagcgaa
    56641 ggtctgatgg atttgagcaa actggaaggt gcgttcagcg atccgacaat gcagttctat
    56701 ctctgcggcc cggttggctt catgcagttt accgcgaaac agttagtgga tctgggcgtg
    56761 aagcaggaaa acattcatta cgaatgcttt ggcccgcata aggtgctgta atttgatgtt
    56821 gccggatgga aacatccggc aacccttgac gcggtttaaa ttgccgcgtc gtcctcttca
    56881 ccggtacgga tgcgaatgac ccgtgccacg tcgaagacga agattttacc gtcaccgatt
    56941 ttgccggttt gcgccgtgcg aataatggta tcaacacagg tatcgacaat gtcgtccggt
    57001 acgacaatct caattttcac tttcggcaga aaatccacca tatactccgc gccgcggtac
    57061 agctcggtat ggcctttctg gcggccaaaa cctttcacct cagtgaccgt catgccggta
    57121 atgccgactt cagccagtgc ttcgcgaaca tcgtccagct tgaagggttt aataatcgca
    57181 tcaatctttt tcatgctatt ccttgaaaag gtcgcctgtc ttttgatctg ctaaacgtaa
    57241 cacataacgc caattcattc cttgaaatcg tttgcatcca gctcgtgtcg ggaaagcagt
    57301 ttataaaatt ctgtccggtt gcgccccgcc attctcgccg cgtgtgtgac gttgcctttg
    57361 gtgatttgca gcagcttacg caaatagttg agttcaaact gattacgtgc ctcaacaaag
    57421 gttggcagcg ccgtattttc accctccagc gcctgctcta ctaatgcatc gctaataacc
    57481 ggagatgagg tcagcgccac gcactgttca atcacgttga ccaactggcg cacattaccc
    57541 ggccagctag cggtcatcag gcgtttcatc gcatcagtgg agaacgcgcg gacaaacggt
    57601 ttatgtcgct ctgccgcctg gcgcaacagg tgatttgcca gaagcggaat gtcttctgtg
    57661 cgctctgcca gagccggaat tttcaggctg acaacgttga ggcggtaata gaggtcttca
    57721 cggaattccc cgcgcgccat cgcttttggc aggtcgcggt gagtggcgga aataatccgc
    57781 acgtcgatat caatatcgcg gttactgccc agcgggcgca ctttacgctc ctgcaacacg
    57841 cgcagcagtt tgacctgcaa cggtgcgggc atatcgccaa tctcatcgag aaatagcgta
    57901 ccgccttccg ccgcctggaa taaaccttcg cgattgctga cagcgccagt aaacgcgcca
    57961 cgcgcatgac caaacagctc cgactccagc aattgctcgg gcaatgcgcc acagttaata
    58021 gcaataaatg gtttgctatt gcgcgggctg gcgttgtgga tagcctgggc gaaaatctct
    58081 ttcccggtgc cgctctggcc gttaatcaaa acgctaacgt ctgattgcgc caccagccgc
    58141 gcctgttcca gcaaacgcag catcagcggg ctgcgggtga caatcgcctc gcgccagcgt
    58201 tcatcggtgg ctggcgcgga ttgttccagc gcatcgtcaa ttgcctgata tagcgcatct
    58261 ttatcgacag gcttggtgag gaaactgaac acgccctgct gtgttgcagc aacggcatcg
    58321 ggaatagaac catgcgcggt aagaataatt actggcattc ccggctgcac tttctgaatt
    58381 tcagcaaaca gctgcatacc gtccatttca tccatccgca ggtcgctgat gactaaatct
    58441 actttttcgc gattcagtac ccgtaatcct tcagcgccac tttccgccgt gaccacgctg
    58501 tagccttcgc tggtcaggcg caggccaagc agtttcagca atcccggatc gtcatcgacc
    58561 aataataaat gcgcaggttt atggctcatc aggagtgacc tcatgggtgg atggcgcggg
    58621 cttttcaccc tcgtgcggcg tatccggcga gaaatttcca gccggtttgc gggtcgagag
    58681 ctggcgttca atatcagtca ggttttccag cttgcgggtg gtgagttcca gttgctgctg
    58741 tagaacgtga tgttgctggc gcaatgtatc cagctcgctg tcgctggcct gctggagctt
    58801 gccgtagcgt tggcgctctt ccgccagttg cagttgcagc gcctgaccgt cgcgccagag
    58861 ttgatacaga gggcgaacct gtgccgggat ctcggtactt agcgcatcaa tgcgcgcgac
    58921 cagctggcgg cgctcatacg gcgtaatttt ggcgtcggcg agcaaaatcc cttgtttaaa
    58981 ggtattttgc cagctgccgt cgtcatattg gcgagcttgc tgacgtgact gcgcaggcat
    59041 taaacgatca gcacaatcca tcgcccgcag ccagtaaagc ggattggttt cggttgattt
    59101 accttgcagc gcccagatgt cgctacattc agtagaaaga tagtcagcca gttgataaac
    59161 cggaattttt tcttctgctg gcgtatcaat ggctggctta ttgtgactct gcacgcaacc
    59221 cagcaatgcc agacatggca gccctgccag ccacagccgt cggggcaata atcgttgaaa
    59281 aatgtgtcgc atattcacca gacttaatgc ctatcccagt gggcgtaatt attgcaggca
    59341 gtttggacat ggacagcgcg gagaaaccgg agcgtacacg tcgtacgtga ggatttcgag
    59401 cactgcccgg ggccgaactg gcaaataaaa tagcctgatg ggataggctg ttagcattat
    59461 ttcgtgtttt tcgacgacgg caattcaatg cggaaacaaa cgtcttgccc gctctcgtcg
    59521 accagataca gttccccttg catacggcaa atacaatccc tggcaatgct taatcccaga
    59581 ccgctgccct tcaccgcccc ttttcgctgg tggcttcctt gaaaaaaggg ttcgaagatc
    59641 atggcgcgtt cctcttgcgg aatgggtgtg cctgtattga tgacatcaat ataaacccgt
    59701 gcaccatgta agctgctgcg aaggcaaatg ttaccggatt cagccccgta gtgcaccgca
    59761 ttggagtaaa gattatccag tacgctcatc agcagcattg gctccgccag gcaagctgtt
    59821 gctttgagat cgacgtcggt atgcatcatt ttagcccgtg cgggcaggct atgagcagaa
    59881 accactgtct ccaccagcgg tgctaattca acattctcca gttccaccgc actgtccgcc
    59941 tgtttacggt tgtaatcaag cagttgttcg atcagttttt gcaaattgcg gctgctgcta
    60001 tcaagaatgc tcaccacctc tttttgctct ggcgtaagcg gcccgacaac ctggtcagcc
    60061 agtaattcag tgccctcacg catactcgcc agtggcgttt ttaattcatg agataaatgt
    60121 cttaaaaatt gatggcgttg ggattccagc catgacaggc gctcacttaa ccaaagaata
    60181 cgttgcccaa ccgagcgtaa ctcgctcggt ccactgaacg aaacgctatt gcccagagaa
    60241 cgcccttccc ccagacggtt gatcatgcgc tcgatatttt tcaccggccc gataatcatc
    60301 cgcgtgaaaa gtagcaccat caccagactc accagaaata gcaccagcga ttgccaacca
    60361 aaatattgcc cacgttcggc gatttcacgc tgaagttgct gcccacgaga gaacaccact
    60421 gtgcgcgtgg cctgcaccat ttcggtattg gcactggcaa aggcttcaag acgcgcggcg
    60481 gcggcagcat cgggaccact gttgttacac tgaagttggg ccagattgtt caaatcctga
    60541 cgcaatgcct ggtaaagttt atcgtctggc agtacgcctg cgtgggcatc gagcatttcg
    60601 ctgtaacgct tgcgctggct ttgataaacc ttcgcaagcg tagggtcgtc cagcacgcaa
    60661 tactgacggt agctacgctc catctccagc gccgcgttgg tcatcgcttc actacgccgg
    60721 gcatcaataa gcgtagtgcg gttaaccagc gccgcctgat cgctaagcgc attcaggctt
    60781 tgccaggctt gccatgccag caccaacagg ggtagtagaa ttagcaaaaa tgccagcatt
    60841 accagttgtc gtaatgagcg gggaaaaacg ggccagcgtt tcaaggtgtt actctcgtca
    60901 gacgcgaata gcctgatgct aaccgagggg aagttcagat acaacaaagc cgggaattac
    60961 ccggctttgt tatggaataa ggcggtgcct aactcgacgt ttcgcccgat ggttgatata
    61021 gctacgctga tatcagaagt tggacggcag gcaccttgtt gtgcgtcatt cgaattttat
    61081 gtagcacatc ccgaaggggc tgacataagt cggtgaatga gccactgctt actattatgc
    61141 agtaactgtg ccaataaaga aaatagtttg gtaacgtact gattatacgt tgctttgagg
    61201 gatttatggc ttcttcgcta tttgaatgta aattagccac tgtgtcgcta aaaagagaca
    61261 acttaagata aacttattag attacaataa aatcaatcat ttaagtgtcg ccagaaagcg
    61321 acacggtaaa ccacccattg tcgtgattta cagacacaaa aaagctcccg gagttgggag
    61381 cttatgatag tggttggtgc ttaatgccgg atgcggcgta aatgccgcat ccgggctgca
    61441 aaaccaatga actgatgact taccccaact gcttacgcgc attgcggaaa atgcgcatcc
    61501 atgggccatc ctcgccccag ttttccggat gccaggagtt gctgacagta cggaaaacac
    61561 gttccgggtg cggcatcata atggtgactc gaccactttc agtcgtaacc gacgtaatac
    61621 cgttcggaga accgttcggg ttagccgggt aggtttcagt gactttgccg aagttatcga
    61681 catagcgcag tgccaccagc cctttgcttt ccagtgccgc cagatgcgcc gcatcacgca
    61741 cttccacgcg cccttcaccg tgagagacag caatcggcat ttgcgagccc accatcccct
    61801 gcaacagcag cgacgggctt tgggtcactt caaccaggct gaaacgcgct tcaaagcgat
    61861 cggaggtatt gcgcacaaaa cgtggccaca actcactacc tgggatcagt tcacgcagat
    61921 tagacatcat ctggcaaccg ttacataccc ccagcgccag cgtttgcgga cggtggaaga
    61981 aggttgcaaa ctcatcgcgt acacggtcat tgaacaggat tgacttcgcc caaccttcac
    62041 cggcacccag cacatcaccg taggagaaac caccgcacgc gaccagggcg tggaaatctt
    62101 ccaggcccgt gcgtccagcc agcaggtcac tcatatgcac gtcgatagca tcaaagcctg
    62161 cacggtggaa agctgccgcc atttcaacat gcgagttcac gccctgctca cgcagtacgg
    62221 caactttcgg gcgcgcgcca gtggcaataa acggtgctgc cacatcttcg ttgatatcga
    62281 acgacagttt tacattcagg cccggatcgg cgtcgtttga tttcgcctgg tgctcctggt
    62341 cagcacactc cgggttgtca cgcaggcgct gcatctgcca ggtagtttct gcccaccaga
    62401 cacgcaacgt agtgcggctt tcgctgaata cagtctgccc gttggcggta atcacaaaac
    62461 ggtcaccgga aaccgcctgc ccaacataat ggacgcaatc agcaagccca tgctgtgcca
    62521 gtacggactc gaccgcttca cggtcagcgg cacgaacctg aatcaccgca cccagttctt
    62581 cgttaaacaa cgccgccagg cgatcgtcac ccagagagcc gatatccgca tcaatgccac
    62641 aatgaccagc aaaggccatt tccgccagtg tgaccaacag accgccatca gagcggtcgt
    62701 gatacgccag cagcttacgc tgtgcaacca gcgcctgaat cgcgtcatag aagcctttca
    62761 gttgcgcgac atcgcgtaca tctgccggtt tgtcgccaag ctgacgataa acctgcgcca
    62821 gcgccgttgc gcccagcgcg ttattgcctt tgcccaaatc aatcagcagc agtgcgttat
    62881 cttcggtaga aagctgcggc gtgatggtgt gacgtacatc ttccacgcgg gcaaatgcag
    62941 aaatcaccag cgacagcggc gacgtcattt cgcgctcttc gttaccttcc tgccagcggg
    63001 ttttcatcga catagagtct ttacccaccg ggatcgtcag gcccagcgcc ggacaaagct
    63061 cttcgcccac ggctttaacg gcttcataca ggcccgcatc ttcaccaggg tggcctgccg
    63121 ccgccatcca gttggcggaa agtttaatgc gtttgatatc gccaatctgt gttgcggcaa
    63181 tattggttaa cgcttcaccg accgccagac gggcagaggc ggcgaaatcc agcagcgcaa
    63241 ccggcgcacg ctcgccaatc gccatcgctt caccgtagta gctgtcgagg ctggcggtag
    63301 tgaccgcgca gttagcgacc ggcacctgcc acggacccac catctgatcg cgcgctacca
    63361 tgccagttac gctgcggtca ccgatggtca ccaggaaagt tttctccgcc acggtcggca
    63421 gatgcagtac gcgtttcacc gcgtctgcaa tggtgatccc ttcacggacc agcgcatcgc
    63481 ccttcgcttt cagcgtttgt acatcgcggg tcatcttcgg cgttttacca agcaggacgt
    63541 ccagcggcag atcgatcggc tgattatcaa aatgacgatc gtgcagagaa agatgcagtt
    63601 cttcggtcgc ttcaccaatc accgcgtagg gtgcgcgctc acgcttacac agttcgtcaa
    63661 acagcggtaa ttgatcggca gcaaccgcca gcacgtagcg ttcctgggat tcgttacacc
    63721 agatttccag cgggctcatg cccggttcgt cgcttagaat atcgcgcagt tcaaatttac
    63781 cgccgcgtcc gccgtcgctc accagctccg gcatcgcgtt agaaagaccg cccgcaccga
    63841 cgtcgtggat aaacagaatt gggttagcat caccaagctg ccagcaacgg tcgatcactt
    63901 cctggcaacg acgctccatc tccgggttgt cgcgctgtac ggaagcaaag tcgaggtcgg
    63961 catcagactg acctgacgcc atggaagacg ccgctccgcc gccaagaccg atgttcattg
    64021 ccgggccgcc gagaacgacc agcttcgcac cgacgttgat ctcgcctttt tgtacgtgat
    64081 cggcgcgaat gttgccgatc ccgcccgcca gcatgatcgg tttgtgataa ccgcgcagct
    64141 cttcgccgtt gtggctgttc actttttctt cataagtacg gaagtagcca ttcagtgccg
    64201 gacgaccaaa ttcgttgtta aacgccgcgc cgcccagcgg gccttcggtc atgatgtcca
    64261 gcgcggtgac aatgcgctca ggcttaccga aatcttcttc ccacggctgt tcgaagccag
    64321 gaattcgcag gttggaaacg gagaaaccaa ccaaacccgc tttcggcttc gcgccgaggc
    64381 cggtggcacc ttcatcgcgg atttcaccgc cggaaccggt cgccgcgccc ggccacggag
    64441 aaatcgccgt cgggtggttg tgagtttcga ctttcatcag aatatgcgcc ggttcctgat
    64501 ggaaatcgta gcggcccgtt tcgtggtcag caaagtagcg gcccacttca gaaccttcca
    64561 ttacggcggc gttatcttta taagcagaga gaacgtaatc tggcgtggtt tcgaaggtgt
    64621 ttttgatcat cttaaacagc gatttcggct gttgctcacc gtcgataacc caatcggcgt
    64681 taaaaatctt gtggcggcag tgctcggagt tcgcctgggc gaacatatac agctcgatgt
    64741 cgttcgggtt acgaccaagc tttgtgaaag catcctgcag atagtcaatt tcatcttccg
    64801 ccagtgccag gccaagacgc aggttagcgt cgattagcgc ctgacggccc tgccccagca
    64861 aatcaacgct ggtgaccgga gtcggttgat ggtgtgcaaa cagctgctct gcatcatcta
    64921 aagcaaaaaa gaccgtttcc atcatgcggt cgtgcagttc agcggtaacc tgctgccatt
    64981 gttcattggt cagcgtaccg gcttctatat agtaagcaac gccgcgctca aggcggttta
    65041 cctgttgtag cccgcagtta tgggcaatat cggtcgcttt cgaagaccag ggagagatgg
    65101 tgccaggacg cggggtcacc agcaggagtt tgccttgcgg ggcgtggctg gcgagtgccg
    65161 ggccatattt cagcaggcgt tcaagttgtg cgtgctcatc atcgtttaac ggcgcattga
    65221 ggtcagcaaa atggacatac tcggcgtaaa tattgtgaac cgggagcctg gcagcctgaa
    65281 aacgtgccag cagtttgttg attcggaatg ccgacagtgc aggcgaacca cgcagaattt
    65341 ccatcataag tctctcgtct tctaagcttt cggtgtaccc aaggggggga aacgggcgtc
    65401 attataaaga atctgatgcg ctgacgaaac cgtttgcgtg gaaataaaat caccatcgtg
    65461 aattagcaac gcgtgccgcc aatggctgta ataagttgcc atctggcgca ggtttacgca
    65521 aaatgccgct catttaagag taaacctttc actattatta cgttttttca agctgggacg
    65581 cgcacgacac agagaattaa ctaattgaaa aaattaaaga ttaattatct gttcatcggc
    65641 attctggcac tgctgctcgc ggtcgctctc tggccatcca ttccctggtt tggtaaagcc
    65701 gacaaccgta tcgccgccat tcaagcgcgg ggagagttgc gtgtgagcac cattcatact
    65761 cccctgactt ataacgaaat caacgggaaa ccttttggcc tggattacga actggcgaaa
    65821 cagtttgccg attacctcgg cgtaaaactg aaagtgaccg tgcggcagaa tatcagccag
    65881 ctgtttgacg accttgataa tggtaacgcc gacctgctgg cggcaggact ggtctataac
    65941 agtgagcggg taaaaaatta tcagcctggc cctacctatt attccgtgtc acaacaactg
    66001 gtttataaag tgggtcagta tcgcccacgt acgctgggca acctgacggc ggagcaactc
    66061 accgttgcac cgggtcatgt ggtggttaac gatctccaga ccctgaaaga aacaaaattc
    66121 ccggaattaa gctggaaggt agacgacaaa aaaggctctg cggaattaat ggaagatgtc
    66181 atcgaaggaa aactcgatta caccattgct gattctgtcg ccatcagcct gtttcagcgc
    66241 gttcacccgg agctcgccgt agcgctcgat atcaccgatg aacaaccggt gacttggttt
    66301 agcccgttag atggcgataa taccctttcc gccgccctgc tcgacttctt caacgaaatg
    66361 aatgaagacg gtacgctggc acgcattgaa gagaaatacc tggggcatgg cgatgatttt
    66421 gattacgtcg atacgcgcac atttttacgc gccgtcgatg cggtactgcc gcagttaaag
    66481 cccctgtttg agaaatacgc cgaagaaatt gactggcgtt tgctggccgc tattgcttat
    66541 caggaatcgc actgggatgc acaggccact tcaccgacgg gtgtgcgcgg catgatgatg
    66601 ttaaccaaaa ataccgcgca aagcctcggc attacggatc gtaccgatgc cgaacagagc
    66661 atcagcggtg gcgtgcgtta tttgcaggat atgatgagta aagtgccgga aagtgtgccg
    66721 gagaacgagc ggatctggtt tgccctcgct gcgtacaata tgggctatgc gcatatgctg
    66781 gatgcccgcg ccctgacggc aaaaaccaaa gggaatcctg acagttgggc tgacgtaaaa
    66841 cagcgtctgc ctttacttag ccagaaaccc tattacagca agctgactta cggctacgct
    66901 cgtggacatg aagcctacgc ttatgtcgaa aatattcgta agtatcagat tagcctggtg
    66961 ggttatctgc aagagaaaga gaagcaggct acagaagcgg cgatgcaact ggcgcaggat
    67021 tatccggcgg tatcgcctac ggagttgggc aaagagaaat ttccttttct ctcgtttctt
    67081 tcccagtcgt catcaaacta tttgacccat tctccctctc tgctgttttc caggaaaggg
    67141 agtgaagaga aacaaaatta atccgtcgag gattgcgctt ttttctgcgc tttaatttcc
    67201 tggcggcgca tgcgaaagaa gtcactgagc aacgccgcgc actcatccgc cagtattcct
    67261 tccgtaattt ccactcggtg attcataccc ggatgatgca gcacatccat taaagatccc
    67321 gcagcgccag ttttcgcgtc acgcgcacca aagaccacgc gaccaatgcg actgtggatc
    67381 atcgctccgg cacacattac acatggttca agcgtgacat acaacgtggc gtcgatcaga
    67441 cgataatttt gcatcaccag accaccctgc cgcagggcca tgatttctgc atgtgcggtg
    67501 ggatcatggc gaccaatcgg gcggttccag ccttcgccga ttacccgatt gttatgcact
    67561 aataccgcgc cgaccggcac ttcccgctca tcccaggcac gtttcgccag cgtcagcgcg
    67621 tgacgcatcc agtattcgtg gctaaattcg acttcagaca aaaagaaaac tccggttata
    67681 aaagcgcggc gcattataca cggacgctat gctttactct attccagttg ctggagttca
    67741 ccgcgcgggg ttactcgcca acgatgctga cagaaataaa gcagcggatt gtcctgatta
    67801 ctgtcgctat agccactgta cagccgcagc ggagtgccga ttttgcgctc cagttgtgcg
    67861 accttttcat gtcccagaca acgcatcgtc aatacccaac caccatagcc acgctgaatt
    67921 tggctggcga taagattaac ccgcggcagc cagggcgtat cgaaataaac cgcttcaacc
    67981 agcggctgcg gagagccggt aatcaaccag atatcagcat cggaacttaa caggtaggtg
    68041 gttaatcgct cctgaaccag cggaaaggcg gtaacattgt cgcgaaacca gcgcacgaaa
    68101 tcggcctgca acgtctgtaa acgtgcttcg ctgtgaccaa aagtgcaccc ccacagaagc
    68161 agactcatcg gccagcgtgc cgcacgacct tttatcaata acgcaatggc tataatcggt
    68221 aacaacggca ggacaagtaa cgcattcagc ggttggcgac gcagtaaata gcgcagaaaa
    68281 ctgccgaaca tatcctgctg atgcaatgtt ccatctaagt caaaaaacac cacacgacgc
    68341 tcgtgagttg ccaaaccata ctcctctgga ctgaaacatc ctgattaatt ttcgctacta
    68401 catagcctaa cagatagatc atcacttttc cggcaacaat cgaccgcatc atggctaact
    68461 ggaattttta attcatggca attagcggca atggaatata aaattcactc gcgtgtctct
    68521 catattgcct gttgtcgcca ctatttcatc gcgaggaaag atatgaacgg cttacttcgt
    68581 atccgtcagc gttaccaggg gcttgcccag agtgataaaa aactggcgga ttatctgctg
    68641 ctacaacctg atacggcgcg ccatttaagc tctcagcaac tggccaacga agccggagtc
    68701 agtcagtcca gcgtcgtgaa gttcgcgcaa aaactcggct ataaaggttt tccggcgctt
    68761 aagttggcgt tgagtgaagc gctggcaagc cagccggaat cgccctccgt gcccattcat
    68821 aaccaaatcc gcggtgatga tccgttacgg ctggtcggcg aaaaactgat taaagaaaat
    68881 actgccgcta tgtacgcaac gctaaacgtt aatagtgaag agaaactgca tgaatgcgta
    68941 acaatgttgc gctctgcgcg gcggataatt ctgaccggta ttggcgcttc gggtctggtg
    69001 gcgcaaaact ttgcctggaa gctgatgaag attggcttca atgctgccgc agtgcgcgat
    69061 atgcatgcgc tactcgcaac agtacaggcg tcgtcccctg acgatctgtt attagccatt
    69121 tcctacaccg gtgtacgacg cgagttaaac ctggcggcag atgagatgct gcgagtgggc
    69181 ggaaaagtgc tggcgattac cggctttact ccgaatgccc tgcaacagcg tgcttctcat
    69241 tgcctgtata ccattgccga agaacaagcg acaaacagcg cttcaatctc tgcttgtcac
    69301 gctcagggaa tgttaacgga tttgctgttc attgcgctga ttcagcagga tctggaactg
    69361 gcaccagaac gtattcgtca tagtgaagcg ctggtgaaaa aactggtctg agtaaagaat
    69421 gcgcgtataa tgcccgcccg gtttgtgttg ttttgagagt ttccttatgg cgttgttaat
    69481 cactaaaaaa tgcatcaatt gtgatatgtg tgaacccgaa tgcccgaatg aggcgatttc
    69541 aatgggagat catatctacg agattaacag cgataagtgt accgaatgcg tagggcacta
    69601 cgagacacca acctgccaga aggtgtgccc gatccccaat actattgtga aagatccggc
    69661 gcatgtcgag acagaagaac agttgtggga taaatttgtg ctgatgcacc acgcggataa
    69721 aatttaatat tctactctgg aagtagagta ttaattatat tactgggaag ccttaacgcc
    69781 attatattta tttaattgat gacattagca taatcattca ctaagttaat ttatatagta
    69841 tctgccaaga cacttattta tagttattaa aggcgcgtcc gattggttca ccggacgcac
    69901 cttaagtacg tttccttgtg ttataagaac agaaggatca gctgtaaaac agcaatgatg
    69961 attttgatga cccgtttaat caggtatcgg caatcagtca ttcgtttttc cttaaacaag
    70021 gaatgcagcc gttcagatta gcccttacat ctccccaaaa ctgaacgtgc gagttattga
    70081 gggtgcatgc tgcactccac accagagctt tgacgacacc actcgtttca atgggggaat
    70141 tctgtggcat ggtgtaaagc acagcaaaat cttcaataac gaagccaatt ttaatgtact
    70201 tacgaattgg cggtcacgtt aatctttcca tcaatattgc tttcttcgta aaggctcgag
    70261 tttttatgct aaagattgca agttgcttgt aaaagataag tacactgatc cataatcgct
    70321 gttgttgagg gtgcatgctg cacaaaatta aagttaaaaa gtaaaacccc cgttccttac
    70381 cagttcgggg gttttacttt ttaaagagaa cggtattatt ttaactttca ataattaccg
    70441 tggcacaagc atagtggcgt tcatctgcca gcgttacatg catatttaca acgccgagct
    70501 tttccgccag ttttaatgcc tcaccccata gccgtagccg tggtttgccg agctcatcat
    70561 tgaatacttc aaattgatta aacgccagac cattgcggat cccggtgcca aacgcttttg
    70621 ctgcggcttc tttcacagca aaacgcttcg ccagaaaacg caccggctgg tggtgcgttt
    70681 tccagattgc ccattcgtta tcgcttaata cgcggcgtgc caggcgctca ccggatcggg
    70741 cgatcactgc ttctatgcga gcgatctcta cgatatccgt gcctaaacct aatattgcca
    70801 ttagccacgc gcttccagca tcagacgctt catttctgcc accgcatctt tcagtccggt
    70861 catcactgca cgaccaataa tggcatgacc gatattcagt tcatgcatct cagggatggc
    70921 ggcaatagct ttcacgttgt gataggtcag accgtgacca gcgttaactt tcagaccgag
    70981 actagcggca aaggtcgcgg ctttggcgat acgcgccagc tcttgcgcct gttcggcgtc
    71041 agttttggca tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc
    71101 cgcagccttg atctgctctt catcggcgtc gataaagaga gaaacctgga tcccggcatc
    71161 tgccagacgt ttgcaggcat cgcgcatttt gtcacgctgc cctgcgacgt ccaggccgcc
    71221 ttcggttgtt acttcctgac gcttttccgg taccagacag caaaaatgtg gcttcgtctc
    71281 aacggcgatc gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag
    71341 cgtctgacgc aggatgcgca catcacggtc agtaatgtgg cggcgatctt cacgtaaatg
    71401 cacggtaatg ccgtccgctc ccgcctgctc agcaataaac gccgcctgca ccggatccgg
    71461 gtaagcagta ccgcgtgcgt tacgcagcgt tgcaatatgg tcaatgttga cgcctaacag
    71521 taattcagcc atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata
    71581 aactgccgga acagttccct gctctttaat ggtttaccgc caagatacgg cttaagcgcc
    71641 atgcgggtaa agcgtttcgc ggcgcgtagt gtgtctgcgt caggaaattc ccgcgcgttt
    71701 aacgctttta actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac
    71761 cctttttctt cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca
    71821 caatgggtaa aattgacgcc atagcccaga tgcccgagca gtgccagttc aaagcggcgc
    71881 agcgcgggtt ctggcgtacc agtggcacct gcaagagact gaatgcagtg caagtaatcg
    71941 aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg
    72001 tacagaccgc tgtaaagcgt gataccgctt aatggcagcg ccagcgagac agcttcagca
    72061 ctgcgcagcg ttttgacttc gccacgcccg ccgaaacgta gcaagagagg ggtgaaaggc
    72121 tgtaatgcac ctttcagagt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg
    72181 cgacccgatt cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta
    72241 tgcaggacaa atgcgcgctg ccagccttcc atcggagtga ctcttaaaga tcgtcaacgt
    72301 aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca
    72361 gttcaaggtg gacaggcgct tcgaacattt cctgcatgtc tttacgcgct tcaatcccga
    72421 tggttttgat cttggcccct ttgttgccaa taaccatctt cttctgccct tcacgctcaa
    72481 cgagaatcaa accattgatg tcataaccac cgcgttcgtt agagacgaaa cgttcgatct
    72541 ccacggtcac ggagtacggc agttcagcgc cgaggaaacg catcagtttt tcgcggatga
    72601 tttcagacgc cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatggg
    72661 tcgcttcagg gagatgctta cgcacgattg ctgcgatagt gtcgacattc agcccggttt
    72721 cagcagagat cggcacgata tcgaggaagt tcatctgact tgccaggaac tgtaagtgcg
    72781 gcagcagatc ggctttttcc tgcacgttat ccactttgtt caccgcgagg atcaccggcg
    72841 ctttgccatc gcgcagtttg ttgagcacca tttcgtcgtc cggcgtccag cgggtgcctt
    72901 caacgacaaa aatcaccagc tcaacgtcgc caatagagct gctcgccgct ttgttcatca
    72961 ggcggttaat agcgcgtttt tcttccatat gcaggccagg ggtatcaacg tagatcgcct
    73021 gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg agttgtttgc gctttgcggg
    73081 aggtgatgga gatcttctgc cccagcagtt tgttcaacaa tgtggatttg ccaacgttcg
    73141 gacgtccgac gatggcaata aatccgcagt aacttttatc gatgctcatt ccagctccag
    73201 ttttttcaac gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc
    73261 aaccaccggt tcactcaggc cgctgacctg gcagtggata gtaaattcct gatcgtgcgc
    73321 ttcgccacgt acctggacta ccagataagt cggcagcggc agatggcgac cctgcaaata
    73381 ttcttgcaag cgcgttttcg gatctttttg tttatcgcct gggctaattt cgtccaaacg
    73441 agtttgatac cagttgagga ttaatttctc gacggtttga atatcactgt cgaggaatac
    73501 accaccaatt aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct
    73561 tttaagttca cctggcccta aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc
    73621 cgccagcgta ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac
    73681 acgagggaaa cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcgcctaa
    73741 aaattctaaa cgctcgttat gtttgctgct ggcactacga tgagttaatg cctgctgcaa
    73801 cagttcctga tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg
    73861 gttcatgcga taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata
    73921 tacatggctc tgctaactgc ttcgctgcag tttcagttta ccggtatatg gggaccaacg
    73981 ctgtttcgtg tgccgtggca acctggaggt gccaacctta aacttcgggg gaatattcta
    74041 tacacaacga cgggggatgt cgttagccac gggagattta tctcataaat aattcacgtt
    74101 gtcgccataa cggcgacaac gtgaacgaag atgggctatt aatggatgcc gccaatgcga
    74161 cttaagcgca cacctgtcgg ccattcgcct tcttgcttat cgaaactcat ccagatagcc
    74221 gttgcccgac cgaccagatt cgcttcaggc acaaaacccc agtaacggct gtccgcgctg
    74281 ttgtcgcggt tgtcgcccat catgaagtat tgtcccggcg gaacaatcca ggttgccagt
    74341 tgttgccctg gctgctggta atacatcccc acctggtcct gcgcaatcgg cactgtcaga
    74401 attcggtgcg tcacatcacc cagtgtctct ttacgctcgg aaagacgaat tccattttct
    74461 ttggtttcgt ttttcggcac ttcaaagaat ccgctggtcg cttccccacc attacggcgt
    74521 gagaaggtct gaacgaaatc gctcggttcc acgttggagt aggtgaccgg tagcgcgttt
    74581 tcacacgcct ggccggaact gcatcccggt tgaatcgtca gctcttttga gaccggatcg
    74641 taagtgactt tatcgcccgg taaacccacc gcgcgcttga tgtaatcaag ctttggatct
    74701 tccggatatt taaagaccac gatatcgccg cgtttcggat gaccggtttc gatcagcgtt
    74761 ttctggtaga taggatcttt aatgccataa gcaaacttct ctaccagaat aaaatcaccg
    74821 attaacagag tcggcatcat cgaacctgac gggatctgga acggttcata aataaacgaa
    74881 cgtacaatca atacgatagc cagcaccgga aaaacagaag caccggtttc cagccagcca
    74941 ggcttcggcg caaccttttt cagggttgct ttatccagtg agtcaccggc agccgcctgc
    75001 gccgctgcct gacgttcccg ccgtttaggt gcgaaaaaga atttatccac gcaccataaa
    75061 atgcccgtca ccagtgtggc aatcaccaga atcagggcaa acatattcgc catgccaact
    75121 cctaagggtt atttgttgtc tttgccaacg tgcagaatgg cgaggaacgc ttcctgcggc
    75181 agctcgacgt taccgatctg cttcatgcgt ttcttacctt ctttctgctt ctgcagcagc
    75241 tttttcttac ggctgatatc gccgccataa catttagcca gtacgttttt acgtagctgt
    75301 ttcacggtgg atcgcgcaat aatgtgcgta ccaatcgctg cctgaatggc gatatcaaac
    75361 tgctggcgtg ggatcagatc tttcatcttc tccaccaact cgcgaccgcg gttttgcgaa
    75421 ttatcacggt gggtgatcag cgccaacgca tcaacacgtt caccgttgat taatacgtct
    75481 acacgtacca tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga cgcataacca
    75541 cgcgaggtag atttcaggcg atcgaagaag tcgagcacca cttccgccat cgggatctcg
    75601 tacgtcagcg ccacctgatt accgtggtaa accatattgg tctgcacgcc gcgtttttct
    75661 acgcacagcg taataacgtt gccgagatat gcctgcggca gcagcatgtg acattcggca
    75721 atcggctcgc gcagttcgta gatgttattt accgctggca gcttggatgg actgtcgacg
    75781 taaataactt ctcttgacgt ggtttcaact tcatacacta cggtcggcgc agtggtgatc
    75841 agatccagat cgtattcacg ttccagacgt tcctggatga tctccatgtg cagcaggccg
    75901 aggaagccgc agcggaaacc aaagcccagc gcgctggagc tttccggctc atagaacagt
    75961 gaagcatcgt tcaggctgag tttacccagc gcgtcacgga aggcttcata gtcgtcggaa
    76021 cttaccggga acagaccggc gtatacctgc ggtttgactt tcttaaagcc aggcagcgcc
    76081 ttttctgccg gattacgcgc cagcgttaag gtatcgccga ctggagcgcc gtggatatct
    76141 ttaatcgcac ataccagcca gcctacttcg ccacatttca gttcagtgcg gtcaacctgt
    76201 ttcggcgtga agatgcccag acggtcggcg ttataggtct gcccggtact catgactttc
    76261 actttgtcgc ccttacgcag ggtgccgttt ttaatacgga taagtgaaac aacgcccagg
    76321 tagttgtcga accatgagtc gataattagt gcctgcaacg ggccttccgg atcgccttcc
    76381 ggcggcggaa tgtcgcgcac cagacgttcg agaacgtcct gcacgccaac gccggttttc
    76441 gctgaacagc gcaccgcgtc ggtggcgtcg atgccgacga tatcttcaat ttcttccgcc
    76501 acgcgttcag gatcggctgc cggcaggtca atcttgttca gtaccggcac cacttcgaga
    76561 tccatttcca tggcggtgta gcagtttgcc agggtttgcg cttctacgcc ctgcccggcg
    76621 tcgaccacca gcaatgcacc ttcacaggca gccagcgaac gggaaacttc ataggagaag
    76681 tcaacgtggc ccggggtgtc gataaagttc agctgatagg tttcgccgtc agacgctttg
    76741 tagtccagcg tcacgctttg cgctttgatg gttatgccac gctcacgctc aagatccatg
    76801 gaatcgagaa cctgcgcctc catttcacgg tcagacaggc caccgcagat ctggataata
    76861 cggtcagaca gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa gttacgtata
    76921 ttcttcataa agtatgatta ttgtgcctta atgcccggtt aaccaggctt ttagaagtcg
    76981 ctgttctgag cttaacgtct gtattaatag aaacgccgca ttctacacta caacattgag
    77041 gcgaggaaat gttcataccg tatggattgt ggtatctgga aacgtcctcg catttgttat
    77101 gcaaaatgca acaaagccag tgaaatcact ggctcgcgtc ttccgaagat gtttcaaatc
    77161 gcacaaggcc aggcggcaat gccacgctta agatgatcgg ttgccattct gcccgggcag
    77221 caaacttacg cgagtagccg cgcgcaatca ggaacccgcc aataccgccg aggaccgcac
    77281 cacataatgc tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg atgaggaata
    77341 atcccaccag cggcgacata taaaccagta atgcggagct aagcaggctg ccttcggcga
    77401 tccccaattc cactttttgc cccggcacta acggctcatc acagggtacg acaatggtat
    77461 gcgtggtttg cgggccaagt ttatttaaca cgcggctacc gcaaccggcg cgtgaagcgc
    77521 agctgctgca tgaaacttta acatcacaac tgaccagcgc ctgcccgttt tgccaggaga
    77581 cgacggtagc ccactctttg atcattgcgc tgccccgaac ttaatattct cggcaatgcg
    77641 tttcgccgtt tgcggcggca gttcaccgac aatggtgatt tcggcgttat cacgtacgct
    77701 tgtactgacg gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg gcgtagcgcg
    77761 gttaacgttt accgagaagc tgaataatcc gtcggaatag agacgtgatt cgataggcat
    77821 gttgtccatc gtcggtagcg gacgtcgact actggaaact tcgctaaaac cctgtggcaa
    77881 ccaggttggc gtccagctga atttagcttt ttcacctaca ggaacagaaa gcaacggcgg
    77941 caaatttgcc ttcgccagcg tctgcatact gctactgata tcctgattga cgttaaaagc
    78001 aatcacgcga aattgttcca gcgtttcacc atcgcgatca aggagatcaa ccctcatcgg
    78061 taatttcgat tcgatgtcca tccacacgat gtagctgtag cgtgtaccat ctcgggcaac
    78121 cacgcgaatg acttcgcaaa gacgatcagc aatacgcgtg cgcccgacgg agataaagtc
    78181 gtagtaagga gaaaggcgtt tgaaatcggt atagatgagc gatggcagag aatcaacaat
    78241 gtaatcgcca ttaagcgtga acggttcaag tcccggttca aaatagctga tttcattgcc
    78301 acgctgtacc acttcccggc gcgggccatc catttgcaac aattgtgcaa gaggacggtt
    78361 atcgaggcgt gcatgtcgat aacgcagaga ctcaacaccc tgtttattga tgctgatgaa
    78421 tgacagctcg taattcagtg actgactggc caggttcatc tgctgtaata acgccccgga
    78481 cgcgggagtg gccgaggcgt tagcagagaa taacaggcta cctgtcacta atgacatggc
    78541 aaaccaaagt tgcttcatta ctgcgattgc gttcctaaag tttgaattcc tggcacctgt
    78601 acagcggctt gctgggtttg cgcctgctca aactgaagct gttcagagtg gagtcggcgt
    78661 tgcagttcgt aatcctgcaa cattgcatta atgcgacgac gctgctcctg tacctgctgc
    78721 tgttgaccat tgtttgcggt cgcttcagaa ggtactccca ggcttaccgg gctggcttta
    78781 cccatcatcg gcagtgtatt aaataccggc gtttcgggct gctgggacgt ttcagattgt
    78841 ccattatagt gctggacgcc aacgataact gcaagcgata cgcatgctgc tacgcccatt
    78901 tgggtaagct gtgccgccca cggacgtact ttctgccaga atggcatttt ctgccattga
    78961 tgcggcgcag gctgggcttc cgggatcaat gtcgccggtt gacgtactgg ctcttcttca
    79021 atggcggcca tcacgcgtga agagatatcg aaatggagca cctcgggagt atcaccccgc
    79081 attgagtcac ggattaagtg atagctttcc caggttttct gcatttctgg gttatgagcc
    79141 agttcgttaa gcagctcact atccagcgtt tcgccatcca ttaaagcgga aagttgttct
    79201 ttctgcatgc ctaataccct tatccagtat cccgctatcg tcaacgcctg ataagcggtt
    79261 gaactttgtt atcaatagct tccctcgctc ggaagatacg tgaacgcacc gtacctaccg
    79321 gacaatccat gatagcggct atctcttcat agctcaggcc atccagctcc cgcaaggtta
    79381 ttgccatgcg caaatcttcc gggagggact caatagttcg gaaaactatc tgtctcagtt
    79441 cttctgacaa cattaagttc tcagggttcg aaatttcttt caacgcgccg ccactttcga
    79501 agttttcagc ttcaatggca tccacatcac tggaaggtgg acgacgcccc tgagcaacca
    79561 ggtaattttt cgctgtattt acagcaatcc gatacagcca tgtataaaaa gcgctatctc
    79621 cccggaacga atccagcgca cgataggctt taataaaagc ttcttgtacc acatcgggaa
    79681 catcacccga cggcacatag cgggaaacca gactcgccac tttatgctga tagcgcacta
    79741 ccagtaggtt aaaggctttc tgatctccct tctggacccg ttcaaccagg acctggtccg
    79801 ttaactgctc gctcatccga ggtaaagtct ccccaaacca aatttccacg cgctatcgaa
    79861 acgccactcc attagctgca atttgagcaa gcaaagggtt agagtgtctc gtttttgtaa
    79921 agttccgtaa cgcatctgtt tttgtttgtc atgctgtaga cggatcatta tctatcatta
    79981 taagtctaca gaatctgaac atcgcattat ctgtgtagaa atgcccattt aactgcctga
    80041 agagtaaccc aacggccttt ttatttcacc acctaatcct ccaccagcca gtaacttctc
    80101 tttttctcgc cgccctgcgt cagcgtgttt agccactgta acaaatatta aaatagcagg
    80161 tgtttattcg cacaacatga tgctatgctg accaaacggt gtttagtaaa ttaaacaaag
    80221 aaaatgaata ctctcccaga acattcatgt gacgtgttga ttatcggtag cggcgcagcc
    80281 ggactttcac tggcgctacg cctggctgac cagcatcagg tcatcgttct aagtaaaggc
    80341 ccggtaacgg aaggttcaac attttatgcc cagggcggta ttgccgccgt gtttgatgaa
    80401 actgacagca ttgactcgca tgtggaagac acattgattg ccggggctgg tatttgcgat
    80461 cgccatgcag ttgaatttgt cgccagcaat gcacgatcct gtgtgcaatg gttaattgac
    80521 caaggggttt tgtttgatac ccacgttcaa ccgaatggcg aagaaagcta ccatctgacc
    80581 cgtgaaggtg gacatagtca ccgtcgtatt cttcatgccg ccgacgccac cggtagagaa
    80641 gtacaatcca cgctggtgag caaggcgcag aaccatccga atattcgtgt gctggagcgc
    80701 agcaacgcag ttgatctgat tgtttctgac aaaattggcc tcccgggcac gcgacgggtt
    80761 gttggcgcgt gggtatggaa ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca
    80821 gtggtactgg caaccggcgg tgcgtcgaag gtttatcagt acaccaccaa tccggatatt
    80881 tcttctggcg atggcattgc tatggcgtgg cgcgcaggct gtcgggttgc caatctcgaa
    80941 tttaatcagt tccaccctac cgcactgtat cacccacagg cacgcaattt cctgttaacg
    81001 gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg ttttatgccc
    81061 gattttgatg agcgcggcga actggccccg cgcgatattg tcgcccgcgc cattgaccat
    81121 gaaatgaaac gcctcggcgc agattgtatg ttcctcgaca tcagccataa gcccgccgat
    81181 tttattcgcc agcatttccc gatgatttat gaaaaattgc tcgggctggg gattgatctc
    81241 acacaagaac cggtaccgat tgtgcctgct gcacattata cctgcggtgg tgtaatggtt
    81301 gatgatcatg ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc
    81361 ggcttacacg gcgctaaccg catggcctcg aattcattgc tggagtgtct ggtctatggc
    81421 tggtcggcgg cagaagatat caccagacgt atgcctgatg cccacggcgt cagtacgtta
    81481 ccgccgtggg atgaaagccg cgttgagaac cctgacgaac gggtagtaat tcagcataac
    81541 tggcacgagc tacggctgtt tatgtgggat tacgttggca ttgtgcgcac aacgaagcgc
    81601 ctggaacgcg ccctgcggcg gataaccatg ctccaacaag aaatagacga atattacgcc
    81661 catttccgcg tctcaaataa tttgctggag ctgcgtaatc tggtacaggt tgccgagttg
    81721 attgttcgct gcgcgatgat gcgcaaagaa agccgggggc tgcattttac gctggattac
    81781 ccggaactgc tcacgcattc cggtccatcg atcctttccc ccggcaatca ttacataaac
    81841 agataaaaag cctgggtcag cgccgtatac gcttcggaat agttctggtc tggcccacga
    81901 atgactaagc gatcgctaaa gcattctccc gcctgcgggg agaatgccag cagcacccga
    81961 tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg ccagcccatg
    82021 cttaatgcca gttcagtaaa accattacca atctgctctg gcagcaccac gcagaaaaat
    82081 ccctcttcgg taatgcactc cgccgcacag gtcagcagcg atgggtgatc aagcgtagtg
    82141 gtatagcgag cctgttcccg ttgaggtgtc gcgcactcta ctccctgctg atagtaaggt
    82201 gggttactga tgattaaatc gaagcgtact gtctgctgtg tgatccactg ctgaatatcc
    82261 gccgtatgga cgttaatccg ctctgcccac ggggactggt tgatattttc ctgcgcctgc
    82321 gcggcagctt cactttccag ctcaacggca tcaattatta cgctgtcact ggttcgctgc
    82381 gccagcatca gtgccagcaa cccgctaccc gcgccgatat caaggcaacg tttcacccca
    82441 gccaccggtg cccatgcgcc cagcaaaatg ccatccgttc ccactttcat cgcacagcga
    82501 tcgtgagcaa caaaaaactg tttaaaagta aatccattac gacgaagcac ggatgtagac
    82561 tgtgacatga aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg agaacaatac
    82621 ccgagaagcg atatccatac aaacagatga agattgcagc cgtaacgtct ataatcagcg
    82681 ccccacacag aggtagaaca tgactgtaac gactttttcc gaacttgaac tcgacgaaag
    82741 cctgctggaa gccctccagg ataaaggttt cactcgcccg accgccattc aggctgccgc
    82801 cattccgcct gcgctcgatg gccgtgatgt actcggttct gcgccgacag gcaccggtaa
    82861 aacggcggcg tatctgctgc cagcgttgca gcacctgctc gatttcccgc gtaagaaatc
    82921 cggtccgccg cgtattttga tcctcacccc aactcgcgag ctggcgatgc aggtggccga
    82981 tcatgcccgc gaactggcga aacatacgca tctggatatc gccaccatca ccggcggcgt
    83041 agcctatatg aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac
    83101 cggacgtctg ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct
    83161 gatcctcgac gaagcagacc gtatgctgga tatgggcttc gctcaggata tcgaacatat
    83221 tgctggcgaa acgcgctggc gtaaacagac cctgctcttt tcggcaacgc tggaaggcga
    83281 tgcgattcag gactttgccg agcgtctgct ggaagatccg gtggaggttt ctgccaatcc
    83341 gtccacccgt gagcgcaaaa aaattcatca gtggtattac cgcgccgatg acctggaaca
    83401 taaaaccgca ttgctggtgc atctgttaaa acagccggaa gcgacccgct caattgtgtt
    83461 tgtgcgtaag cgtgaacgtg tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa
    83521 caactgctat ctcgaaggtg agatggtaca aggcaagcgt aacgaagcga tcaagcgttt
    83581 gaccgaaggt cgcgtaaacg tactggttgc aaccgatgtt gccgcgcgcg gtatcgacat
    83641 tcctgacgtc agccacgtct ttaacttcga tatgccgcgc agtggcgata cttatttgca
    83701 ccgtatcgga cgtaccgcgc gcgccggtcg taaaggcacc gcaatttcgc tggtggaagc
    83761 ccatgaccat ttgctgctgg gcaaggttgg ccgttacatt gaagagccga ttaaagctcg
    83821 cgttattgat gagttacgcc cgaaaacgcg tgcgccaagc gagaagcaga ccggcaagcc
    83881 atcgaagaaa gtactggcaa aacgcgccga gaagaaaaaa gctaaagaga aagagaaacc
    83941 gcgtgtgaaa aaacgccatc gcgacaccaa aaatattggt aagcgccgta aaccaagcgg
    84001 aacgggcgtg ccaccgcaaa cgacagaaga gtaatctcaa tgccgggttt aagcctggta
    84061 ttaaaagtgc tggcaaacgc aaaactgcct gatgcgctac gcttatcagt atctacgtgg
    84121 ctcatgcaat atattgaatt tgcacgatct tgtaggccgg ataaggcgtt cacgccgcat
    84181 ccggcatgaa taaagcgcac tttgtcgaca agttaaatgc cgggtttaat cccgacatta
    84241 tttttatcca gccacaaaac tctcttcaac acactgaata aaagtgtgca tcgccgggct
    84301 aaccgctttt ccagcatggt gtgcgcacat ggccgtaatg gtctgcgact gttcgccaaa
    84361 aggcaattca attaattctc cgctctcaag ctctttttcc accgcaaagc ggggcaagta
    84421 actgacaccg atattcgccg cgacacaacg tttgatactt tcaatgctta acagctcaat
    84481 ggtgttttcc accgtgatcc gccgctgacg cagcgtgctc tcaaatatct gccggaagac
    84541 acattgcggt tcgttgataa taaagctact ggcgttatgt cttcccggct cagtaaaatc
    84601 gacatctgca atttgcggtg aagccaccag caccagcgat tgttcaccca actctcgtcg
    84661 gttcagggca tcatcattcc ctacgcgata aaagacgccg acatcggcct catcattcag
    84721 tagcgcatca cggatcacgt aacagttcag cgactgcaac gataaacgca cttttggtgc
    84781 gcgttgccga aaacgctgca acacctgcgg cattcggtaa gagagcaacg tttcgcccga
    84841 aacaacgcgt agttccccgt ccggatctga ctcctttttg gcagcctcac gaagcgtatc
    84901 catcactcgg gtaagttcat aaatgtgcgg cagcaacttt tttccttcgc gggtaaggca
    84961 catccgtcgg ccaattttct caaataactg gactgaaaac tcctgctcaa gctgctgaat
    85021 atgaaaagtc accgtcgatt gtgtacagca caatttttgc gaagctcgca aaaaggaacc
    85081 ctcttccacc acggttttaa gcgtaataaa acggcgcaga tccataaccc caaacctatc
    85141 gaaaatatcg aatctagaat ataaaaacat tcattttttt aaatgttccg tgtcgggtac
    85201 tgtctaccaa aacagaggag ataacaagtg acaccgaccc ttttaagtgc tttttggact
    85261 tacaccctga ttaccgctat gacgccagga ccgaacaata ttctcgccct tagctctgct
    85321 acgtcgcatg gatttcgtca aagtacccgc gtgctggcag ggatgagtct gggatttttg
    85381 attgtgatgt tactgtgtgc gggcatttca ttttcactgg cagtgattga cccggcagcg
    85441 gtacaccttt tgagttgggc gggggcggca tatattgtct ggctggcgtg gaaaatcgcc
    85501 accagcccaa caaaggaaga cggacttcag gcaaaaccaa tcagcttttg ggccagcttt
    85561 gctttgcagt ttgtgaacgt caaaatcatt ttgtacggtg ttacggcact gtcgacgttt
    85621 gttctgccgc aaacacaggc gctaagctgg gtagttggcg tcagcgtttt gctggcgatg
    85681 attgggacgt ttggcaatgt gtgctgggcg ctggcggggc atctgtttca gcgattgttt
    85741 cgccagtatg gtcgccagtt aaatatcgtg cttgccctgt tgctggtcta ttgcgcggta
    85801 cgcattttct attaacgaaa aaaagcggaa gaggtcgccc tcttccgctt agtaacttgc
    85861 tacttaagcc ttacaggctt tcagtaaagg tacgagcgat aacgtcgcgc tgctgttccg
    85921 gagtcagaga gttaaagcga actgcataac cggatacacg gatggtcagc tgcggatatt
    85981 tttccggatg cttaactgca tcttccagag tttcacgacg cagaacgtta acgttcaggt
    86041 gttgaccacc ttcaacgcga acttctggtt tcacttctac tggaacttca cggtattcaa
    86101 tgtcacccag tttgcttact gcaaccactt catcttctgc ataacctgct tttgcaacga
    86161 tgcaacgtgc ttcgcctttt tcgctgtcca gcagccagaa agagttcagc agatcgtcgt
    86221 tagcggcttt agtaatctgg atacctgtaa tcatgtgatg cctccccggc aaaattattt
    86281 gatttgttca gcctgtcgcg gccaattggt aaaaccattg ttgcttgagt gtatatatac
    86341 tcctcaacca cccttgattc tttgatttaa atcaataaaa accacacatc gattgtggtc
    86401 gtaaatggat tttattgttt tacatcaact tatgcgggtg tgaaatttta ccaatttaca
    86461 tttttttgca ctcgtttaag tctaaaaaat gagcatgatt ttgttctgta gaaagaagca
    86521 gttaagctag gcggattgaa gattcgcagg agagcgagat ggctaacgaa ttaacctggc
    86581 atgacgtgct ggctgaagag aagcaacaac cttattttct taataccctt cagaccgtcg
    86641 ccagcgagcg gcagtccggc gtcactatct atccaccaca aaaagatgtc tttaacgcgt
    86701 tccgctttac cgagttgggt gacgttaaag tggtgattct cggccaggat ccttatcacg
    86761 gaccgggaca ggcgcatggt ctggcatttt ccgttcgtcc cggcattgcc attcctccgt
    86821 cattattgaa tatgtataaa gagctggaaa atactattcc gggcttcacc cgccctaatc
    86881 atggctatct cgaaagctgg gcgcgtcagg gcgttctgct gctcaatact gtgttgacgg
    86941 ttcgcgcagg tcaggcgcat tcccacgcca gcctcggttg ggagacgttt actgataaag
    87001 tcatcagcct gattaaccag caccgcgaag gcgtggtgtt tttgttgtgg ggatcacatg
    87061 cgcaaaagaa aggggcgatt atagataagc aacgccatca tgtactgaaa gcaccgcatc
    87121 cgtcgccgct ttcggcgcat cgtggattct ttggctgcaa ccattttgtg ctggcaaatc
    87181 agtggctgga acaacgtggc gagacgccga ttgactggat gccagtatta ccggcagaga
    87241 gtgagtaaat ttgcggggaa atgccggatg gcagagttgc cacccggctg atttatcagg
    87301 ctttattctg acgccaccat tcaccaagca aaacgccggt tgcgacagag atattcagcc
    87361 cggcaacgtt gcccgtaccg tcaatcttca cgcgcagatc gttcggatcg cgtgcggcat
    87421 ccggtaaccc ttcatattcc tgacccagca ccagtaccat tttcgctggc agactagttt
    87481 tgaacagcgg tttaccctgc tcgctggaag tggtcactac ggtgtaacct gcctgacgga
    87541 aatcatccag cacgttaaca atgttgtcgc cagtaatcgg ctgaacgtgc tctgcgccgc
    87601 cttctgcggt acggatagcc gcccccgact ccagcagtgc cgcatcctgc accacgacac
    87661 ctttcacgcc gaagtgcgcg cagctgcgca tcatgccacc caggttatgc gggttagatt
    87721 cgttttccag tgccagaacg caatcctgcg cgcctgcctg gcttacccac tgctgcacgg
    87781 ttgtaccgtt acgctttttg atcaagaagc aaacgccgcc gtgatgttcc gtgcctgacg
    87841 cttttgtcaa ttccgcttca tccaccacat ggtacgcttt gcggtttgct gccatccagc
    87901 gcaaggcttc tttaaaacgc ggcgttacac tctggataaa ccaggcgcga acaatcgctt
    87961 ccggacggct ctggaaaagt gcctgacatg cattttcgcc gtagacgcgg gtttcttccg
    88021 cacgctgacg acgcaacact tccggatcaa taaaactttt accactgatg ccaccgtgat
    88081 cggccttttc cggcgtctca tcacccggcg cgcgggaaac cgtgcgccac ggcgaatctt
    88141 cccacttgcg gtcacggggc tgactctgtt tgtcatcgcg ggcggggcga cggccaccgt
    88201 cagcacgaga ttttcctgga cgcccgcccc ctttcccggt acgcgggttg tgggtacgtt
    88261 tatcagaatc atcatcactg cggacataca tcactttgac cttgccgctt ttacctttca
    88321 tttcatcgtt catgcttttc tccaccagcg ctgcgcgaag cgcgcagatt acccgaagtc
    88381 cgcgcggttc gccatgattt cgtaccaaag cctgcgacta tcatacctat tgaataaaac
    88441 agattgttgt ctggaacaat gtccccgata atatgtaaca tattagaaac ataccggcgt
    88501 cgttgccgat aagtctcctt actcatcccg aggttagtta tgaataccgt ttgtacccat
    88561 tgtcaggcca tcaatcgcat tcccgacgat cggatcgaag atgcggcaaa atgcggacgc
    88621 tgcggtcacg acttgtttga cggagaggtg attaatgcga ccggtgaaac actcgacaaa
    88681 ttgctgaagg atgatctacc tgtggtgatc gacttctggg caccgtggtg cggcccctgc
    88741 cgtaatttcg caccaatttt tgaagatgtc gcgcaagagc gtagcggtaa agtgcgcttt
    88801 gtgaaagtga ataccgaagc tgaacgtgaa ttgagcagtc gctttggaat tcgtagtata
    88861 ccgacgatca tgattttcaa aaacggtcag gttgtcgaca tgcttaatgg cgcagtaccg
    88921 aaagcgccgt tcgatagctg gctgaacgaa tctctttaat cttaccgggg cgcatcttgt
    88981 gccccgtttt ctcctctgcg acaatggcgt tttttcgacg ctctcttatg accgaaaacg
    89041 gtgttctcca gttacgcgcc gagcgtattg cgcgcgcaac acgtcctttt cttgcccgcg
    89101 gtaatcgcgt tcgtcgctgc caacgctgtc ttttgccaga gaaattatgt ctctgttcga
    89161 caattacccc agcacaagca aaaagtcgct tctgtttgct gatgttcgac accgagccaa
    89221 tgaagcccag taataccggg cgtctgattg ctgatatttt gcctgatacc gttgcgtttc
    89281 aatggtcgcg taccgaaccc tcgcaggatt tgctggagtt ggtgcaaaac ccggactatc
    89341 agccaatggt ggtctttccc gcttcgtatg ctgatgagca acgggaagtg atcttcacac
    89401 cacctgccgg taagccaccg ctgtttatca tgctcgatgg tacctggccg gaagctcgca
    89461 agatgtttcg taaaagtccg tatctggata atcttcccgt catttccgtc gatctttccc
    89521 ggctttctgc ctatcgcctg cgtgaagccc aggctgcagg ccaatattgt actgccgagg
    89581 tagccatcgc actgttagat atggccagcg ataccggggc ggcagcaggg ttaggcgagc
    89641 attttacccg ctttaaaaca cgctatctgg caggaaaaac gcaacatctg ggtagcatca
    89701 cagcagaaca gttagaaagc gtttaaaatc attcggtcac ttctgcggga gaccggtatg
    89761 agtcagcgag gactggaagc actactgcga ccaaaatcga tagcggtaat tggcgcgtcg
    89821 atgaaaccca atcgcgcagg ttacctgatg atgcgtaacc tgctggcggg aggctttaac
    89881 ggaccggtac tcccggtgac gccagcctgg aaagcggtgt tgggtgtgtt ggcctggccg
    89941 gatattgcca gcttgccctt tacacccgac cttgcggttt tatgtaccaa tgccagccgt
    90001 aatcttgctc ttctggaaga gctcggcgag aaaggctgta aaacctgcat tattctttcc
    90061 gccccggcat cgcaacacga agatctccgc gcctgcgccc tgcgccataa catgcgcctg
    90121 cttggaccaa acagtctggg tttactggct ccctggcaag gtctgaatgc cagcttttcg
    90181 cctgtgccga ttaaacgcgg caagctggcg tttatttcgc aatcggctgc cgtctccaac
    90241 accatcctcg actgggcgca acagcgtaag atgggctttt cctactttat tgcgctcggc
    90301 gacagcctgg atatcgacgt tgatgaattg cttgactatc tggcacgcga cagtaaaacc
    90361 agcgccatcc tgctctatct cgaacagtta agcgacgcgc gacgctttgt ttcggcggcc
    90421 cgtagtgcct ctcgcaacaa accgattctg gtgattaaaa gcggacgtag cccggcggct
    90481 caacgtctgt tgaacaccac ggcaggaatg gatccggcgt gggatgcggc tattcagcgt
    90541 gccggtttgt tgcgggtaca ggacacccac gaactgtttt cggcggtgga aacccttagc
    90601 catatgcgcc cgctgcgtgg cgaccggttg atgattatca gcaacggtgc tgcgcctgcc
    90661 gcgctggcgc ttgatgcctt atggtcacgc aatggcaagc tggcaacact aagcgaagaa
    90721 acctgccaga aactgcgcga tgcactgcca ggacatgtgg caatctctaa cccgctcgat
    90781 ctgcgcgatg acgccagcag tgagcactat attaaaacgc tggacattct gctccacagc
    90841 caggattttg atgcgctgat ggttattcat tcgcccagcg ccgctgctcc cgcaacagaa
    90901 agcgcgcaag tattaattga agcggtaaag catcatcccc gcagcaagta tgtttctctg
    90961 ctgacgaact ggtgtggcga acattcctcg caagaggcac gacgattatt cagcgaagcc
    91021 gggctgccga cctaccgtac cccggaagga accatcactg cttttatgca tatggtggag
    91081 taccggcgta atcagaagca actacgcgaa acgccggcgt tgcccagcaa tctgacctcc
    91141 aataccgcag aagcgcatct tctgttgcaa caggcgattg ccgaaggagc aacgtcactc
    91201 gatacccatg aagttcagcc tatcctgcaa gcatatggca tgaacacgct ccccacctgg
    91261 attgccagcg atagcaccga agcggtgcat attgccgaac agattggtta tccggtggcg
    91321 ctgaaattgc gttcgccgga tattccacat aaatcggaag ttcagggcgt catgctctac
    91381 ctgcgtacag ccaatgaagt ccagcaagcg gcaaacgcca ttttcgatcg cgtaaaaatg
    91441 gcctggccgc aggcacgggt ccacggcctg ttggtgcaaa gtatggctaa ccgtgctggt
    91501 gctcaggagt tgcgggttgt ggttgagcac gatccggttt tcgggccgtt gatcatgctg
    91561 ggcgaaggcg gtgtggagtg gcgtcctgaa gatcaagccg tcgtcgcgtt accgccgctg
    91621 aacatgaacc tggcccgcta tctggttatt caggggatca aaagtaaaaa gattcgtgca
    91681 cgcagtgcgc tacgcccatt ggatgttgca ggcttgagcc agcttctggt gcaggtttcc
    91741 aacttgattg tcgattgccc ggaaattcag cgtctggata ttcatccttt gctggcttct
    91801 ggcagtgaat ttaccgcgct ggatgtcacg ctggatatct cgccgtttga aggcgataac
    91861 gagagtcggc tggcagtgcg cccttatccg catcagctgg aagaatgggt agaattgaaa
    91921 aacggtgaac gctgcttgtt ccgcccgatt ttgccagaag atgagccaca acttcagcaa
    91981 ttcatttcgc gagtcaccaa agaagatctt tattaccgct actttagcga gatcaacgaa
    92041 tttacccatg aagatttagc caacatgaca cagatcgact acgatcggga aatggcgttt
    92101 gtagcggtac gacgtattga tcaaacggaa gagatcctcg gcgtcacgcg tgcgatttcc
    92161 gatcctgata acatcgatgc cgaatttgct gtgctggttc gctcggatct caaagggtta
    92221 ggcttaggtc gacgcttaat ggaaaagttg attacctata cgcgagatca cggactacaa
    92281 cgtctgaatg gtattacgat gccaaacaat cgtggcatgg tggcgctagc ccgcaagctc
    92341 gggtttaacg ttgatatcca gctcgaagag gggatcgttg ggcttacgct aaatcttgcc
    92401 caacgcgagg aatcatgagt aaggtactgg aaatgttgac cacttaatcg ggactggtgt
    92461 tattattgcc cgcttatgtc gtctgcattg cacagaggac ccttcaatga acagagaaga
    92521 aatgcactgt gatgttgtca aaatttaagc gtaataaaca tcaacaacac cttgcccaac
    92581 tacccaagat ttctcaatca gttgatgatg tcgatttctt ttacgctccc gccgacttcc
    92641 gggagacgct gctggaaaaa atagccagcg cgaagcagcg catttgcatt gtcgccctgt
    92701 atctcgaaca ggatgacggt ggcaaaggca ttctgaacgc gttgtatgaa gctaaaaggc
    92761 agcgtccgga actggatgtg cgggtgctgg tcgactggca tcgtgcacaa cgtggacgca
    92821 ttggcgctgc ggcatctaac actaacgctg actggtactg ccgcatggcg caggaaaatc
    92881 cgggcgtaga tgttccggtt tatggcgttc caatcaatac ccgtgaagcc cttggtgttc
    92941 tgcactttaa aggctttatc atcgacgata gcgtacttta tagcggtgcc agcctgaacg
    93001 atgtttacct gcatcagcac gataaatatc gctacgaccg ttatcatctt atccgtaacc
    93061 gtaagatgtc agacattatg tttgaatggg ttacacagaa tattatgaat ggccgcggcg
    93121 ttaatcgtct ggatgatgtt aatcggccaa aaagcccgga aatcaagaac gatattcgtc
    93181 tgttccgcca ggagctgcgt gatgccgctt atcatttcca gggcgatgcc gacaacgatc
    93241 agctttccgt aacgccgcta gtggggctgg ggaaatcgag tctgttgaac aagaccattt
    93301 tccatcttat gccttgtgcc gagcagaaac taaccatctg tacgccatac ttcaacctgc
    93361 cagcaatcct tgtgcgcaat attatccagt tgctgcgcga agggaaaaag gtcgaaatta
    93421 ttgttggtga taaaaccgcg aacgacttct acattccgga agatgaacct ttcaagataa
    93481 ttggcgcatt gccttatctc tatgagatca atctgcgtcg tttcctgagc cgtttgcagt
    93541 attacgtcaa tactgaccag ctagtggttc ggttatggaa agatgacgac aacacctatc
    93601 acctaaaagg gatgtgggtt gatgataagt ggatgttgat caccggtaat aacctgaacc
    93661 cgcgcgcctg gcgtctggat ctggaaaacg ccattttgat ccacgatcca caactggagc
    93721 ttgcgccgca gcgagagaaa gaactggagc tgatccgcga gcataccacc atcgttaagc
    93781 actatcgcga tctgcaaagt attgccgatt atccggtgaa ggttcgtaaa ctcatccgcc
    93841 gtttgcgccg tatccgcatc gaccgattaa ttagccgcat cctgtaatca caaccccgtc
    93901 ctgtacgggg tttgtttttt ggaggccacg ttttgcgtat tctttttgtc tgttcgctat
    93961 tgttactttc tggatgcagc catatggcta acgatagctg gagcgggcag gataaagctc
    94021 aacactttat cgcctcggcg atgctttccg ccgccggaaa tgaatattca cagcatcagg
    94081 ggatgagccg ggatcgcagt gccatgtttg gattgatgtt ctctgtcagt ttgggggcgt
    94141 caaaagagct ttgggatagc cgccccgaag ggagcggctg gagctggaag gatttggcct
    94201 gggatgtcgc cggtgcaagc accggctata ccgtctggca actgacccgt cactaaagac
    94261 gcatcccctt ccctttgcga tgtagcatca aagaaaccag aaacgccacc acggccatca
    94321 aggtcacata ccagaagaag gctgtttcca ttcctattga tttcagcgac aacgctacgt
    94381 actccgccga accaccaaat atagcattag cgaccgcata tgacaggcca acgcctaatg
    94441 cgcgaacctg tgccgggaac atctcagcct tcagtattcc actgattgat gtataaaaac
    94501 tcactatcag cagggcacac atcaccagac caaaagcggc ataaggcgag gaaacgtttt
    94561 gcaatgctga gagaatagga acggtaaaaa tggctgccag cgaaccgaaa cataacattg
    94621 aggtacggcg accaatctta tccgacagcg cgccaatgag tggttgaata agcatgaata
    94681 caaacaatgc ggcagtcata atgccactcg ccacgttggc atgcattccc gcagtattta
    94741 ccagatactt ctgcatataa gtggtgaagg tatagaaaca aagggagccc gcagcggtaa
    94801 aaccgagaac catgatgaat gcacggcgat tgcgccataa tcctttcaga gatccagctt
    94861 cttttaaagc gcgcgtttct tgttgcgaag tttcatctaa ttgacgacgt aaccacaacg
    94921 ccacaacagc taacacagct cctaacgcga aaggaatacg ccatccccac tctctgagtg
    94981 cagcgtcttc catggtgtgt tgtaaaacca cgacaaccag tagggctagc agttgtccgc
    95041 cgatcaacgt cacatactga aatgatgcgt aaaaaccttt gcgcccttca acggcaactt
    95101 cactcatata ggtggcgctg gtgccatatt ctccgccaac ggataatccc tgaaataaac
    95161 gagcgagaag caataatgcc ggagcccacg tacctatagt ttcataacct gggaggcagg
    95221 cgataaccag cgatccgaaa cacatcatac acaccgataa cagcatcgat tttttgcgac
    95281 catgtttatc ggctatgcgg ccaaatagcc aaccgcctat tgggcgcatc aggaatcccg
    95341 cagcaaaaac acctgctgtt tgtagtagtt gagtcgtcgt gttcccggaa gggaagaaaa
    95401 tgtgggcgaa gtagagtgaa cagaacgagt agacatagaa atcgaaccac tcgaccagat
    95461 tacctgaaga ggcccccaca atcgcccaaa tgcggcgacg tgtatcacta cttgtcagtt
    95521 tgctgtctgc cgttacagta ctttcagcca tgccattatg tctcctgccg taatccgatg
    95581 cttttgtcgg tcgcttttgt ttatttcttt tggtaaagaa aatgttatac atttgttgcg
    95641 tatcattatg caaccttaac catgaattta gttagcagga aaatggttat tgaggagctt
    95701 aaggataaat ttctggtgag gagaacacgt atggaggtgg gcaagttggg gaagccgtat
    95761 tcgctgctga atctggcata tgtgggagta taagacgcgc agcgtcgcat caggcatttt
    95821 tttcaccaac gcaaaaaggc catcctgac
//
LOCUS       DAZKGS010000003        83997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKGS010000003 DAZKGS010000000
VERSION     DAZKGS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            243..3341
                     /gene="ygfK"
                     /locus_tag="ACHOQY_000196"
     CDS             243..3341
                     /gene="ygfK"
                     /locus_tag="ACHOQY_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9676360.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            3344..4672
                     /gene="ssnA"
                     /locus_tag="ACHOQY_000197"
     CDS             3344..4672
                     /gene="ssnA"
                     /locus_tag="ACHOQY_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9676361.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            4723..5502
                     /gene="ygfM"
                     /locus_tag="ACHOQY_000198"
     CDS             4723..5502
                     /gene="ygfM"
                     /locus_tag="ACHOQY_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9676362.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            5499..8369
                     /gene="xdhD"
                     /locus_tag="ACHOQY_000199"
     CDS             5499..8369
                     /gene="xdhD"
                     /locus_tag="ACHOQY_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9676363.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            8534..9934
                     /gene="xanQ"
                     /locus_tag="ACHOQY_000200"
     CDS             8534..9934
                     /gene="xanQ"
                     /locus_tag="ACHOQY_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9676364.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            9952..11268
                     /gene="guaD"
                     /locus_tag="ACHOQY_000201"
     CDS             9952..11268
                     /gene="guaD"
                     /locus_tag="ACHOQY_000201"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9676365.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            11304..12671
                     /gene="ghxQ"
                     /locus_tag="ACHOQY_000202"
                     /gene_synonym="ygfQ"
     CDS             11304..12671
                     /gene="ghxQ"
                     /locus_tag="ACHOQY_000202"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9676366.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(12707..13195)
                     /gene="ygfS"
                     /locus_tag="ACHOQY_000203"
     CDS             complement(12707..13195)
                     /gene="ygfS"
                     /locus_tag="ACHOQY_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9676367.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(13195..15114)
                     /gene="ygfT"
                     /locus_tag="ACHOQY_000204"
     CDS             complement(13195..15114)
                     /gene="ygfT"
                     /locus_tag="ACHOQY_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9676368.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            15550..16998
                     /gene="uacT"
                     /locus_tag="ACHOQY_000205"
     CDS             15550..16998
                     /gene="uacT"
                     /locus_tag="ACHOQY_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9676369.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            17000..17125
                     /gene="yqfG"
                     /locus_tag="ACHOQY_000206"
     CDS             17000..17125
                     /gene="yqfG"
                     /locus_tag="ACHOQY_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9676370.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17122..17193)
                     /locus_tag="ACHOQY_000207"
     CDS             complement(17122..17193)
                     /locus_tag="ACHOQY_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676371.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            17248..17796
                     /gene="idi"
                     /locus_tag="ACHOQY_000208"
     CDS             17248..17796
                     /gene="idi"
                     /locus_tag="ACHOQY_000208"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9676372.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(17839..19356)
                     /gene="lysS"
                     /locus_tag="ACHOQY_000209"
     CDS             complement(17839..19356)
                     /gene="lysS"
                     /locus_tag="ACHOQY_000209"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9676373.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(19366..20464)
                     /gene="prfB"
                     /locus_tag="ACHOQY_000210"
     CDS             complement(join(19366..20388,20390..20464))
                     /gene="prfB"
                     /locus_tag="ACHOQY_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9676374.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(20555..22288)
                     /gene="recJ"
                     /locus_tag="ACHOQY_000211"
     CDS             complement(20555..22288)
                     /gene="recJ"
                     /locus_tag="ACHOQY_000211"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9676375.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(22294..23004)
                     /gene="dsbC"
                     /locus_tag="ACHOQY_000212"
     CDS             complement(22294..23004)
                     /gene="dsbC"
                     /locus_tag="ACHOQY_000212"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9676376.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(23029..23925)
                     /gene="xerD"
                     /locus_tag="ACHOQY_000213"
     CDS             complement(23029..23925)
                     /gene="xerD"
                     /locus_tag="ACHOQY_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9676377.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            24037..24558
                     /gene="fldB"
                     /locus_tag="ACHOQY_000214"
     CDS             24037..24558
                     /gene="fldB"
                     /locus_tag="ACHOQY_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9676378.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(24598..25005)
                     /gene="ygfX"
                     /locus_tag="ACHOQY_000215"
     CDS             complement(24598..25005)
                     /gene="ygfX"
                     /locus_tag="ACHOQY_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9676379.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(24986..25252)
                     /gene="sdhE"
                     /locus_tag="ACHOQY_000216"
     CDS             complement(24986..25252)
                     /gene="sdhE"
                     /locus_tag="ACHOQY_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9676380.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            25495..26475
                     /gene="ygfZ"
                     /locus_tag="ACHOQY_000217"
     CDS             25495..26475
                     /gene="ygfZ"
                     /locus_tag="ACHOQY_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9676381.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(26671..27330)
                     /gene="yqfA"
                     /locus_tag="ACHOQY_000218"
     CDS             complement(26671..27330)
                     /gene="yqfA"
                     /locus_tag="ACHOQY_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9676382.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(27494..27805)
                     /gene="yqfB"
                     /locus_tag="ACHOQY_000219"
     CDS             complement(27494..27805)
                     /gene="yqfB"
                     /locus_tag="ACHOQY_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9676383.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            27850..29283
                     /gene="bglA"
                     /locus_tag="ACHOQY_000220"
     CDS             27850..29283
                     /gene="bglA"
                     /locus_tag="ACHOQY_000220"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9676384.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(29340..30083)
                     /gene="ygfF"
                     /locus_tag="ACHOQY_000221"
     CDS             complement(29340..30083)
                     /gene="ygfF"
                     /locus_tag="ACHOQY_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9676385.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(30344..33217)
                     /gene="gcvP"
                     /locus_tag="ACHOQY_000222"
     CDS             complement(30344..33217)
                     /gene="gcvP"
                     /locus_tag="ACHOQY_000222"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9676386.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(33336..33725)
                     /gene="gcvH"
                     /locus_tag="ACHOQY_000223"
     CDS             complement(33336..33725)
                     /gene="gcvH"
                     /locus_tag="ACHOQY_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9676387.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(33749..34843)
                     /gene="gcvT"
                     /locus_tag="ACHOQY_000224"
     CDS             complement(33749..34843)
                     /gene="gcvT"
                     /locus_tag="ACHOQY_000224"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9676388.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            35134..35217
                     /locus_tag="ACHOQY_000225"
     CDS             35134..35217
                     /locus_tag="ACHOQY_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676389.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(35291..36493)
                     /gene="ubiI"
                     /locus_tag="ACHOQY_000226"
                     /gene_synonym="visC"
     CDS             complement(35291..36493)
                     /gene="ubiI"
                     /locus_tag="ACHOQY_000226"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9676390.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLCNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(36516..37694)
                     /gene="ubiH"
                     /locus_tag="ACHOQY_000227"
                     /gene_synonym="visB"
     CDS             complement(36516..37694)
                     /gene="ubiH"
                     /locus_tag="ACHOQY_000227"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9676391.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(37691..39016)
                     /gene="pepP"
                     /locus_tag="ACHOQY_000228"
     CDS             complement(37691..39016)
                     /gene="pepP"
                     /locus_tag="ACHOQY_000228"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9676392.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(39042..39620)
                     /gene="ygfB"
                     /locus_tag="ACHOQY_000229"
     CDS             complement(39042..39620)
                     /gene="ygfB"
                     /locus_tag="ACHOQY_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9676393.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            39788..40117
                     /gene="zapA"
                     /locus_tag="ACHOQY_000230"
     CDS             39788..40117
                     /gene="zapA"
                     /locus_tag="ACHOQY_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9676394.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            40159..40342
                     /gene="ssrS"
                     /locus_tag="ACHOQY_000231"
     ncRNA           40159..40342
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOQY_000231"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            40363..40965
                     /gene="fau"
                     /locus_tag="ACHOQY_000232"
     CDS             40363..40965
                     /gene="fau"
                     /locus_tag="ACHOQY_000232"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9676395.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(41066..41125)
                     /locus_tag="ACHOQY_000233"
     CDS             complement(41066..41125)
                     /locus_tag="ACHOQY_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9676396.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(41458..41517)
                     /gene="ibsC"
                     /locus_tag="ACHOQY_000234"
     CDS             complement(41458..41517)
                     /gene="ibsC"
                     /locus_tag="ACHOQY_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9676397.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(41746..42978)
                     /gene="serA"
                     /locus_tag="ACHOQY_000235"
     CDS             complement(41746..42978)
                     /gene="serA"
                     /locus_tag="ACHOQY_000235"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9676398.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(43234..43893)
                     /gene="rpiA"
                     /locus_tag="ACHOQY_000236"
     CDS             complement(43234..43893)
                     /gene="rpiA"
                     /locus_tag="ACHOQY_000236"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9676399.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(43949..44134)
                     /locus_tag="ACHOQY_000237"
     CDS             complement(43949..44134)
                     /locus_tag="ACHOQY_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676400.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            44276..45169
                     /gene="argP"
                     /locus_tag="ACHOQY_000238"
     CDS             44276..45169
                     /gene="argP"
                     /locus_tag="ACHOQY_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9676401.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            45373..47517
                     /gene="scpA"
                     /locus_tag="ACHOQY_000239"
     CDS             45373..47517
                     /gene="scpA"
                     /locus_tag="ACHOQY_000239"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP9676402.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            47510..48505
                     /gene="meaB"
                     /locus_tag="ACHOQY_000240"
     CDS             47510..48505
                     /gene="meaB"
                     /locus_tag="ACHOQY_000240"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP9676403.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            48516..49301
                     /gene="scpB"
                     /locus_tag="ACHOQY_000241"
     CDS             48516..49301
                     /gene="scpB"
                     /locus_tag="ACHOQY_000241"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP9676404.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            49325..50803
                     /gene="scpC"
                     /locus_tag="ACHOQY_000242"
     CDS             49325..50803
                     /gene="scpC"
                     /locus_tag="ACHOQY_000242"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP9676405.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(50800..51696)
                     /gene="ygfI"
                     /locus_tag="ACHOQY_000243"
     CDS             complement(50800..51696)
                     /gene="ygfI"
                     /locus_tag="ACHOQY_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9676406.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(51863..52603)
                     /gene="yggE"
                     /locus_tag="ACHOQY_000244"
     CDS             complement(51863..52603)
                     /gene="yggE"
                     /locus_tag="ACHOQY_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9676407.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELAHGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(52696..53331)
                     /gene="argO"
                     /locus_tag="ACHOQY_000245"
     CDS             complement(52696..53331)
                     /gene="argO"
                     /locus_tag="ACHOQY_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9676408.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(53470..54330)
                     /gene="mscS"
                     /locus_tag="ACHOQY_000246"
     CDS             complement(53470..54330)
                     /gene="mscS"
                     /locus_tag="ACHOQY_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9676409.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            54526..54624
                     /locus_tag="ACHOQY_000247"
                     /pseudo
     CDS             54526..54624
                     /locus_tag="ACHOQY_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(54688..55767)
                     /gene="fbaA"
                     /locus_tag="ACHOQY_000248"
     CDS             complement(54688..55767)
                     /gene="fbaA"
                     /locus_tag="ACHOQY_000248"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9676410.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(55982..57145)
                     /gene="pgk"
                     /locus_tag="ACHOQY_000249"
     CDS             complement(55982..57145)
                     /gene="pgk"
                     /locus_tag="ACHOQY_000249"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9676411.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(57195..58214)
                     /gene="epd"
                     /locus_tag="ACHOQY_000250"
     CDS             complement(57195..58214)
                     /gene="epd"
                     /locus_tag="ACHOQY_000250"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9676412.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            58586..59017
                     /locus_tag="ACHOQY_000251"
     CDS             58586..59017
                     /locus_tag="ACHOQY_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP9676413.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            59040..59618
                     /locus_tag="ACHOQY_000252"
     CDS             59040..59618
                     /locus_tag="ACHOQY_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9676414.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            59619..60326
                     /locus_tag="ACHOQY_000253"
     CDS             59619..60326
                     /locus_tag="ACHOQY_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9676415.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            60314..60991
                     /locus_tag="ACHOQY_000254"
     CDS             60314..60991
                     /locus_tag="ACHOQY_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9676416.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            60985..61662
                     /locus_tag="ACHOQY_000255"
     CDS             60985..61662
                     /locus_tag="ACHOQY_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9676417.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(61634..62347)
                     /locus_tag="ACHOQY_000256"
     CDS             complement(61634..62347)
                     /locus_tag="ACHOQY_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9676418.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(62344..62853)
                     /gene="fumE"
                     /locus_tag="ACHOQY_000257"
     CDS             complement(62344..62853)
                     /gene="fumE"
                     /locus_tag="ACHOQY_000257"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9676419.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(62875..63840)
                     /gene="glpX"
                     /locus_tag="ACHOQY_000258"
     CDS             complement(62875..63840)
                     /gene="glpX"
                     /locus_tag="ACHOQY_000258"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9676420.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(63837..65114)
                     /gene="yggP"
                     /locus_tag="ACHOQY_000259"
     CDS             complement(63837..65114)
                     /gene="yggP"
                     /locus_tag="ACHOQY_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9676421.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(65129..66517)
                     /gene="cmtA"
                     /locus_tag="ACHOQY_000260"
     CDS             complement(65129..66517)
                     /gene="cmtA"
                     /locus_tag="ACHOQY_000260"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9676422.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(66545..66988)
                     /gene="cmtB"
                     /locus_tag="ACHOQY_000261"
     CDS             complement(66545..66988)
                     /gene="cmtB"
                     /locus_tag="ACHOQY_000261"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9676423.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(67302..69293)
                     /gene="tkt"
                     /locus_tag="ACHOQY_000262"
     CDS             complement(67302..69293)
                     /gene="tkt"
                     /locus_tag="ACHOQY_000262"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9676424.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            69571..70329
                     /gene="loiP"
                     /locus_tag="ACHOQY_000263"
                     /gene_synonym="yggG"
     CDS             69571..70329
                     /gene="loiP"
                     /locus_tag="ACHOQY_000263"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9676425.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(70385..71128)
                     /locus_tag="ACHOQY_000264"
     CDS             complement(70385..71128)
                     /locus_tag="ACHOQY_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9676426.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(71115..72224)
                     /gene="dgaE"
                     /locus_tag="ACHOQY_000265"
     CDS             complement(71115..72224)
                     /gene="dgaE"
                     /locus_tag="ACHOQY_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFP9676427.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGVITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(72228..73088)
                     /locus_tag="ACHOQY_000266"
     CDS             complement(72228..73088)
                     /locus_tag="ACHOQY_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9676428.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(73085..73834)
                     /locus_tag="ACHOQY_000267"
     CDS             complement(73085..73834)
                     /locus_tag="ACHOQY_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9676429.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(73860..74345)
                     /locus_tag="ACHOQY_000268"
     CDS             complement(73860..74345)
                     /locus_tag="ACHOQY_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9676430.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(74356..74784)
                     /locus_tag="ACHOQY_000269"
     CDS             complement(74356..74784)
                     /locus_tag="ACHOQY_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9676431.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(74903..77701)
                     /gene="dagR"
                     /locus_tag="ACHOQY_000270"
     CDS             complement(74903..77701)
                     /gene="dagR"
                     /locus_tag="ACHOQY_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFP9676432.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(77960..78880)
                     /gene="speB"
                     /locus_tag="ACHOQY_000271"
     CDS             complement(77960..78880)
                     /gene="speB"
                     /locus_tag="ACHOQY_000271"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9676433.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(79016..79747)
                     /locus_tag="ACHOQY_000272"
     CDS             complement(79016..79747)
                     /locus_tag="ACHOQY_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676434.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(79893..81869)
                     /gene="speA"
                     /locus_tag="ACHOQY_000273"
     CDS             complement(79893..81869)
                     /gene="speA"
                     /locus_tag="ACHOQY_000273"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9676435.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(81878..82009)
                     /gene="yqgB"
                     /locus_tag="ACHOQY_000274"
     CDS             complement(81878..82009)
                     /gene="yqgB"
                     /locus_tag="ACHOQY_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9676436.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(82103..82246)
                     /gene="yqgG"
                     /locus_tag="ACHOQY_000275"
     CDS             complement(82103..82246)
                     /gene="yqgG"
                     /locus_tag="ACHOQY_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9676437.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(82357..82608)
                     /gene="yqgD"
                     /locus_tag="ACHOQY_000276"
     CDS             complement(82357..82608)
                     /gene="yqgD"
                     /locus_tag="ACHOQY_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9676438.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            82664..83818
                     /gene="metK"
                     /locus_tag="ACHOQY_000277"
     CDS             82664..83818
                     /gene="metK"
                     /locus_tag="ACHOQY_000277"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9676439.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT        20250 a        22566 c        21053 g        20128 t
ORIGIN      
        1 tgagaaaaga gaaaaagtga taaatgggct gacaataata attttcagga gtgattttaa
       61 ttctcatttg gcacctgtga gttcattcac aaaaagcctt taagtttcga gccgcctcac
      121 atttttttat atttcccgcc aaacctggca agagtggtgc gattgttgct ctatccccct
      181 aaaccaccgg atttctcaac accggtcact caatgatatc tgtataagct aaggagaggg
      241 ttatggggga tattatgcgt cccattccgt ttgaggaact tttgacgcgc atatttgatg
      301 aataccaaca acaacgctca atctttggta ttcccgagca acagttttac tcacctgtaa
      361 aaggtaaaac tgttagcgtc ttcggtgaaa cctgtgccac tcccgtcggc cctgccgctg
      421 gcccgcacac gcagctcgcg caaaatattg tcacttcctg gctgactggc ggacgcttca
      481 tcgaactaaa aaccgtccaa attcttgacc gcctggagct ggaaaagccc tgtatcgatg
      541 ccgaagacga gtgctttaac accgaatggt ctaccgaatt taccctgctt aaagcctggg
      601 atgaatacct caaagcctgg tttgccctgc accttctcga agcgatgttc cagccttctg
      661 attccggtaa atcgttcatc tttaatatga gcgtcggtta caacctcgaa ggtattaagc
      721 aaccgccaat gcaacagttc atcgacaata tgatggacgc atctgaccat ccgaaattcg
      781 cgcaatatcg cgatacgctg aataaattac tccaggatga cgcattttta gcccgccacg
      841 gattgcagga aaaacgcgaa agcttgcaag ccttacccgc tcgcatcccc accagtatgg
      901 tgcagggcgt caccctctcc accatgcacg gctgtcctcc gcatgaaatc gaagccattt
      961 gccgctacat gctggaagaa aaagggctca acacctttgt gaaacttaac ccgaccttac
     1021 tgggatacgc gcgtgttcgt gagatcctcg atgtctgcgg tttcggttac ataggcttaa
     1081 aagaagagtc atttgatcac gacctcaagc tgacgcaagc actggaaatg ctggaacgcc
     1141 tgatggcact ggcaaaagaa aaatcactcg gctttggcgt aaaactgact aacactctcg
     1201 gcaccatcaa caataaaggc gcactgcctg gtgaagagat gtatatgtca ggccgtgcgc
     1261 tgttcccgct ctccatcaat gttgcagcag ttctctctcg cgcctttgac ggcaaactgc
     1321 ccatttctta ttccggtggt gccagtcagc tgactatccg cgatattttt gatacaggta
     1381 ttcgccctat tactatggca accgacctgc tgaaacctgg cggctatttg cgcttaagtg
     1441 cctgcatgcg cgagctggaa ggctccgacg cctggggact tgaccatgtt gacgtcgaac
     1501 gactgaacag actggcagca gatgcgttaa ccatggaata cacccagaaa cactggaagc
     1561 cagaagagcg tattgaagtg gcagaagacc tgccgctgac cgattgctac gttgccccct
     1621 gtgttactgc ctgcgctatc aagcaagata ttccggaata catccgtctg cttggcgaac
     1681 accgctatgc cgacgcgctg gaactcatct accaacgcaa cgctctgccc gccattaccg
     1741 gtcatatttg cgatcaccag tgccaataca actgtacccg cctggattac gacagtgcgc
     1801 tgaatatccg cgaactgaaa aaagtcgcgc tggaaaaagg ttgggatgaa tataagcaac
     1861 gctggcacaa accagccggt tctggttcac gccatccggt tgccgtgatt ggtgcaggtc
     1921 cggcgggtct ggcagcaggt tacttccttg ccagagcggg ccatccggtt acgctgtttg
     1981 aacgcgaagc caatgcgggc ggcgtggtga aaaatatcat tcctcagttc cgaattccgg
     2041 ctgagttaat tcagcacgat atcgattttg ttgccgatca cggcgtgaaa tttgagtatg
     2101 gctgctcacc cgatttgacc gttgaacagt taaaaaatca gggcttccac tatgttctga
     2161 ttgccaccgg cactgataaa aatagcggtg tgaaactggc gggtgacaac caaaatgttt
     2221 ggaaatcact ccccttcctg cgtgaataca acaagggtac agcgctcaag ctgggcaaac
     2281 atgtggtcgt tgtcggggcg ggtaacaccg caatggactg cgctcgtgcg gcgttacgcg
     2341 ttccaggcgt agaaaaagca acgatcgttt accgtcgttc actgcaagag atgcccgcat
     2401 ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtgga gttccgtttc ctgaataatc
     2461 cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt tatgtcgctt ggcgaacccg
     2521 atgagaaagg ccgtcgccgt ccggttgaaa ccaacgaaac agtaacactg catgtagaca
     2581 gcctgatcac cgccattggt gaacagcagg atactgaagc cctgaatgcg atgggcgtgc
     2641 cgctggacaa aaacggctgg ccagacgtcg accacaatgg cgaaactcgt ctgactgacg
     2701 tctttatgat cggcgacgta cagcgcggac catcctccat tgtcgctgct gtcggaaccg
     2761 cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat ccgttcccac cagaacgata
     2821 aatactggaa caacgtcaat ccggcggaaa tctatcaacg taaaggcgat atctctatca
     2881 ctctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca ggaagccgct cgctgcctcg
     2941 aatgtaacta cgtttgcagc aagtgtgtgg atgtctgccc gaaccgcgcc aacgtctcca
     3001 ttgcggtccc aggcttccag aatagattcc agacgctgca cctcgacgct tactgtaacg
     3061 aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa accatacaaa gacaaaatca
     3121 ccgttttcag cctggcgcaa gactttgata acagcagcaa cccaggcttc cttgtggaag
     3181 attgccgggt acgagtacga ctgaataacc aaagctgggt gttaaacatc gacagcgaag
     3241 gtcagttcaa caacgtacca ccggagctga acgatatgtg ccgcatcatc agccatgtcc
     3301 accagcatca tcattatctg ctgggccgcg tggaggtgta atcatgttga ttctgaagaa
     3361 tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa ggcgttgata tcgctatcga
     3421 aaatgatgtg attgtcgcta tcggcgatgc cctgacgcaa cgctaccccg atgccagcta
     3481 caaagagatg catggtcgga ttgtgatgcc gggaattgtc tgctcgcaca accattttta
     3541 ctcggggctt tcccgcggaa ttatggcaaa catcgccccc tgcccggatt tcatctcaac
     3601 gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat gaagagtcgc tctattacag
     3661 cgggctgatt tgttccctgg aagcgattaa gagcggatgt acatcggtta tcgatcacca
     3721 tgcctctccg gcgtatatcg gcgggtcgct ctccacattg cgcgacgcat ttttaaaagt
     3781 tggcctgcgc gcgatgacct gttttgaaac tactgaccgt aacaacggca tcaaagagtt
     3841 gcaggaaggt gtagaagaaa acatccgctt cgcccgtcag attgatgagg cgaagaaagc
     3901 agcaaccgag ccgtatctgg tggaagcaca tatcggtgct cacgcgccgt ttaccgtgcc
     3961 agatgccggt ctggagatgc tacgtgaagc cgtgaaagcc acaggccgtg gtttgcatat
     4021 tcacgctgcg gaagaccttt acgacgtttc ctacagtcac cactggtacg gcaaagacct
     4081 gctggcacga ctagcgcaat tcgatctcat cgatagcaaa acgctggtcg ctcatgggct
     4141 gtacttgtcg aaagatgaca tcgccctact caatcagcgc gatgcgttcc tggtgcataa
     4201 cgcccgttca aacatgaaca accatgtcgg ctacaaccat caccttagcg acatccgcaa
     4261 tctggcgttg ggaacggacg gcattggttc ggacatgttt gaagagatga aatttgcctt
     4321 ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac agttttgcca aagccctgac
     4381 taacggtaac gaactgatga gccgcaactt tggcgcgaaa tttggacttc tggaagccgg
     4441 ttacaaagct gatttaacca tttgcgatta caactcaccg acgccgctgc tggcagacaa
     4501 tatcgccggg catatcgctt tcggtatggg ctcaggcagc gttcacagcg tgatggtcaa
     4561 tggtgtgatg gtctatgaag accgtcagtt taacttcgat tgcgattcca tttatgcaca
     4621 agccagaaaa gccgctgcca gtatgtggcg tcggatggat gcgctggcat aaatgacaga
     4681 tgccctcttc ccgcagggaa gagggttaaa taaggaaaga agatgattga acaatttttc
     4741 aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc gctaccagga tgaagccgtc
     4801 tggttcgccg ggggcagcaa actcaatgct acaccaaccc gtaccgataa aaagattgcc
     4861 atttccttac aggatctgga actggactgg gttgactggg ataacggtgc actgcggatt
     4921 ggcgcaatgt ctcgcttgca gccactgcgt gatgcgcgat ttattcctgc agcgctgcgt
     4981 gaagccctcg gttttgttta ctcacgccat gttcgtaatc agtcgaccat tggtggtgaa
     5041 atcgccgccc gccaggaaga gtcggtgctg cttcccgtcc tgttggcact ggatgctgaa
     5101 ctggtttttg gcaacggcga aacgctgtca atcgaggact acctggcctg cccatgcgat
     5161 cgcctgctaa ccgaaattat cattaaagat ccgtatcgca cctgtgcgac ccgcaaaatt
     5221 agccgttctc aggcaggttt aaccgtcgtg acggcagccg ttgcaatgac agaccacgac
     5281 ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac tgcgtctgca tgatgtcgaa
     5341 aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg ccaacgccat tttcccgcag
     5401 gaagatttgc ggggcagcgt ggcctataaa cgctatatca cgggagttct ggtagccgac
     5461 ctgtatgccg actgccaaca ggctggggag gaagccgtat gatcatccac tttactttaa
     5521 atggcgcgcc tcaggagcta accgttaatc caggcgaaaa cgtgcaaaag ctgttgttta
     5581 acatgggaat gcactctgta cgcaacagtg atgatggctt cgggtttgcc ggttctgacg
     5641 caataatctt taacggtaat atcgttaacg cgtccttgct tattgccgca cagttagaga
     5701 aggcagatat tcgtaccgca gaatctttgg gcaaatggaa cgagttaagt ctggttcaac
     5761 aggcaatggt tgatgttggc gtggtgcagt ctggttataa cgatccagct gcagctctga
     5821 ttatcaccga tcttctcgat cgcatcgccg cacctacccg cgaagagatc gacgacgcgc
     5881 tttctggttt gttcagccgc gatgcaggct ggcaacaata ctatcaggtc attgaactgg
     5941 cggttgcacg taaaaataat ccgcaggcca ccattgatat cgctccgact ttccgtgacg
     6001 accttgaagt cattggcaag cattatccta aaactgatgc cgcgaaaatg gtgcaggcga
     6061 aaccctgcta tgttgaagac cgcgtaacgg ctgacgcctg cgtcattaaa atgttacgta
     6121 gcccacacgc tcacgcactg attactcatc tggatgtcag caaagctgaa gccttaccgg
     6181 gcgtcgttca cgttattact cacctgaatt gcccggatat ctactatacc ccgggtggtc
     6241 agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt cggcaagaaa atgcgtcacg
     6301 tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga aattgcgctc gaagcattga
     6361 agctcatcga cgttgaatat gaagtgctta agccggtaat gtcgatcgac gaagcaatgg
     6421 cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta tgttgctggt gcgccagata
     6481 ctctggaaga cgataacagc catgcagccc agcgcggcga gcatatgatc atcaacttcc
     6541 cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat tcatggtcat attggcgata
     6601 tggacaaagg ctttgccgat gccgatgtga tcattgagcg aacctataac tcaacgcagg
     6661 cacagcagtg cccgactgaa acacatatct gctttacccg gatggacggc gatcgtctgg
     6721 ttatccacgc ctccacccag gtaccatggc acttacgccg ccaggtcgcg cgcctcgtag
     6781 gcatgaaaca gcataaagtt catgtcatta aagagcgagt tggcggcggt tttggctcca
     6841 aacaggacat cctgctggaa gaagtgtgcg catgggcaac ctgcgtgacc gggcgtccgg
     6901 tactgttccg ctacacccgt gaagaagagt ttattgctaa cacctctcgt cacgtcgcga
     6961 aagtcaccgt caaactggga gcgaaaaaag atggtcgcct gacggcagtg aagatggatt
     7021 tccgcgccaa cactggccct tacggcaacc actcactcac cgtaccgtgt aacggaccgg
     7081 cgctgtcgct gccgttatat ccgtgcgata acgtcgattt ccaggtcacc acctactaca
     7141 gcaacatttg cccaaatggt gcttatcagg gttatggcgc accgaaaggt aacttcgcta
     7201 tcaccatggc attagcggaa ctggctgaac agttacagat cgaccaactg gaaattatcg
     7261 aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct cggtgcaatc ggtgaaggta
     7321 aagcgccgac ctccgttcct tccgccgcca gctgcgcact ggaagagatc ctgcgtcagg
     7381 gtcgcgagat gatccaatgg tcttcaccga aaccgcaaaa tagtgactgg cacatcggtc
     7441 gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga tatcgatcag gctaactgca
     7501 tgatcaaact ggaatcggac ggcaccttta tcgttcattc tggcggtgcg gatattggta
     7561 ctggtctgga tactgtagtg acgaaactgg cagcagaagt gctgcactgc ccaccgcagg
     7621 acgtgcatgt tatctccggt gataccgatc atgcgttgtt tgataaaggc gcatatgcct
     7681 cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc agcggaaaat ctgcgggaga
     7741 aaattctgtt ccacggcgcg caaatgttgg gtgagccagt ggcagatgtt caactagcaa
     7801 cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt cggggatatt gcccataaag
     7861 gcgaaaccgg caccggcttt ggttcactgg tgggaactgg cagttatatc acgcctgatt
     7921 tcgctttccc gtatggcgca aacttcgctg aagttgccgt caacacgcgt acgggtgaaa
     7981 tccgcctgga taaattctac gccttgctgg actgcggtac accggtcaat ccagagttag
     8041 cgttgggaca aatctacggt gccaccctgc gtgctatcgg ccacagtatg agcgaagaga
     8101 tcatttataa cgccgaaggt cacccgttaa cgcgtgattt acgcagttac ggcgcaccga
     8161 aaattggtga cattccgcgt gatttccgcg ctgtgttggt gccgagcgac gataaagtcg
     8221 gcccgttcgg ggcgaaatcg atctcggaaa ttggtgtaaa tggcgcagct ccggcgattg
     8281 ctaccgcaat tcacgatgcc tgcggcatct ggttacgcga atggcatttc acaccggaga
     8341 aaatactcac cgcgctggaa aaaatataag taataaatgg ccgcttcggc ggcctgtttc
     8401 tctgtttgaa cgatgactaa atagtgactg cgaaaatgag ataagccgtt ctcaaggcgt
     8461 cctgcgcccc aaattcgtgg gaatttattt tactaattca gatgatcaaa tttactttaa
     8521 aaggagtgaa gggatgtctg atataaacca tgcaggttct gaccttatat ttgaactgga
     8581 ggatcgccct ccctttcatc aggctctcgt tggtgccatt acccatctgt tggcaatttt
     8641 cgttccgatg gtaacccccg cgttaatcgt gggtgcggcc ttacagcttt ccgctgaaac
     8701 aaccgcctat cttgtttcta tggcgatgat cgcctctggt attggtacct ggttacaagt
     8761 aaaccgctac ggcatcgtcg gttctggcct actctcaatt cagtcagtca atttttcatt
     8821 tgttacggtc atgattgcgc tgggcagcag catgaaaagc gacggttttc acgaagagtt
     8881 aatcatgtcg tcgcttctcg gcgtctcctt cgttggcgca tttctggttg tcggatcttc
     8941 atttatcttg ccctatttac gtcgggttat tacgcctacc gtcagcggta ttgtggtact
     9001 gatgatcggc ttaagcctga ttaaagtcgg cattatcgat tttggtggag gatttgcagc
     9061 caaaagcagc ggtacgttcg gcaattacga acatctcggc gttggtttat tggttttaat
     9121 tgtggtgatc ggctttaact gctgtcgcag tccgttgcta cgcatgggag ggatcgccat
     9181 tgggctatgt gtcggctata tcgcatcgtt atgcctgggc atggtggatt tcagcagtat
     9241 gcgcaatttg ccgttaatca ccatcccgca tccgttcaaa tacggcttta gttttagctt
     9301 ccatcagttc ctggtggttg gcacgattta tctgcttagc gtgctggaag cagtcggcga
     9361 tatcaccgcc acggcaatgg tttcccgccg ccccattcag ggggaagagt atcagtcccg
     9421 actgaaaggc ggcgtgctgg cagatggtct ggtttctgtt atcgcctccg ctgtcgggtc
     9481 attaccctta accacgtttg cgcaaaataa tggggttatt cagatgactg gcgtcgcttc
     9541 acgttatgtc gggcgaacca tcgcggtaat gctggttatc ctcggtttgt ttccgatgat
     9601 tggcggcttc ttcacgacca ttccctcggc agttctggga ggcgcaatga cgttgatgtt
     9661 ttccatgatt gccatcgctg ggattcgcat aatcatcacc aacggtttaa agcgccgaga
     9721 aacacttatt gtcgccactt ctttaggttt aggacttggc gtctcctacg atcccgaaat
     9781 ttttaaaata ttgccagcct ctatttatgt actagttgaa aaccctattt gtgctggcgg
     9841 gttaactgcg attttattaa atattatcct ccctggtggc taccgacagg aaaacgttct
     9901 gcctggtatt acctcagcgg aagagatgga ttaacagtaa aggagtcaat gatgtcagga
     9961 gaacacacgt tgaaagcggt acgaggcagt tttattgatg tcacccgtac ggtcgataac
    10021 ccggaagaga ttgcctctgc gctgcggttt attgaggatg gtttattact cattaaacag
    10081 ggaaaagtgg aatggtttgg cgaatgggaa gacggaaagc atcaaattcc tgacactatt
    10141 cgcgtgcgcg actatcgcgg caaactgata gtaccgggct ttgtcgatac acatatccat
    10201 tatccgcaaa gtgaaatggt gggggcctat ggtgaacaat tgctggagtg gttgaataaa
    10261 cacaccttcc ctactgaacg tcgttatgag gatttagagt acgcccgcga aatgtcggca
    10321 ttcttcatca agcagctttt acgtaacgga accaccacgg cgctggtgtt tggcactgtt
    10381 catccgcaat ctgttgatgc gctgtttgaa gccgccagtc atatcaatat gcgtatgatt
    10441 gccggtaagg tgatgatgga ccgcaacgca ccggattatc tgctcgacac tgccgaaagc
    10501 agctatcacc aaagcaaaga actgattgaa cgctggcaca aaaatggtcg tctgctatat
    10561 gcgattacgc cacgcttcgc cccgacctca tctcctgaac agatggcgat ggcgcaacgc
    10621 ctgaaagaag aatatccgga tacgtgggta catacccatc tctgtgaaaa caaagatgaa
    10681 attgcctggg tgaaatcgct ttatcctgac catgatggtt atctggatgt ttaccatcag
    10741 tacggcctga ccggtaaaaa ctgtgtcttt gctcactgcg tccatctcga agaaaaagag
    10801 tgggatcgtc tcagcgaaac caaatccagc attgctttct gtccgacctc caacctttac
    10861 ctcggcagcg gcttattcaa cttgaaaaaa gcatggcaga agaaagttaa agtgggcatg
    10921 ggaacggata tcggtgccgg aaccactttc aacatgctgc aaacgctgaa cgaagcctac
    10981 aaagtattgc aattacaagg ctatcgcctc tcggcatatg aagcgtttta cctggccacg
    11041 ctcggcggag cgaaatctct gggccttgac gatttgattg gcaacttttt acctggcaaa
    11101 gaggctgatt tcgtggtgat ggaacccacc gccactccgc tacagcagct gcgctatgac
    11161 aactctgttt ctttagtcga caaattgttc gtgatgatga cgttgggcga tgaccgttcg
    11221 atctaccgca cctacgttga tggtcgtctg gtgtacgaac gcaactaata ataaaacttt
    11281 aacatcctcg tgaggacatc attatgtctg gagacatcct acaaacaccg gacgcaccaa
    11341 agccacaggg cgcgctggat aattatttta aaattaccgc tcgtggcagt accgttcgtc
    11401 aggaagtact ggctggctta acgacctttc tggccatggt ttattccgtt atcgtcgttc
    11461 cgggaatgct gggcaaagca ggttttcctc ccgcagctgt gtttgttgcc acctgtctgg
    11521 tcgcgggctt cggctcgttg ctgatggggt tgtgggccaa tttgccaatg gcgattggtt
    11581 gtgcgatttc cttgacggca tttaccgcat tcagtctggt actcgggcaa caaattagcg
    11641 ttcctgtcgc actgggcgcg gtctttctga tgggcgtcat cttcaccgct atttccgtaa
    11701 ccggtgtgcg tacctggatc ttacgtaatt taccgatggg tatcgctcac ggtacaggta
    11761 tcggtatcgg cctgtttctg ctgctgattg ctgctaacgg tgtgggtatg gttatcaaaa
    11821 acccgattga aggcttgcca gtggcgctcg gtgcgtttac ctccttcccg gtgatgatga
    11881 gcttgctggg gctggcggtc atcttcggtc tggagaagtg ccgcgtaccc ggcgggatct
    11941 tgttggtgat tatcgcaatt tcgattatcg gcttaatctt tgacccagcg gtgaaatacc
    12001 acggtctggt ggcgatgcca agtctgactg gcgaagatgg taagtctctg attttcagcc
    12061 tcgatattat gggcgcactc cagccaaccg tacttccgag tgtactggca ttggtgatga
    12121 ccgcagtgtt cgacgccact ggcaccatcc gtgccgtcgc cggtcaggcg aatttgttgg
    12181 ataaagacaa ccagatcatc aatggcggca aagccctgac cagtgactca gtaagttcaa
    12241 tattctccgg tctggtgggc gcagcgcccg cagcggttta tatcgaatca gcggcaggaa
    12301 ccgccgccgg gggtaaaaca gggttgaccg caaccgtagt gggggcgtta ttcctgctga
    12361 ttctgttctt atcaccgctg tcatttttga tccctggtta cgccactgca cccgctctga
    12421 tgtacgtggg tttgctgatg ttaagtaacg tctcgaagct ggatttcaac gattttattg
    12481 acgcaatggc tggcctggtg tgtgccgtgt tcatcgttct gacttgtaat atcgttaccg
    12541 gtattatgct gggctttgtg acactggtcg taggccgtgt ctttgctcgc gaatggcaaa
    12601 agttgaatat tggtacggtg atcattactg ccgcactggt cgcattttac gcgggtggtt
    12661 gggcaatcta atagattctc cgcgcctttc agcgcggaga tttctttcac agaggattct
    12721 caccgctggc aacaatacgc tgctggcgga ctcgcctgag ttctctttca gtaagcaact
    12781 gcagcgcctg tgtcgggcaa gattcaacac aggctggccc ctcttcccgc tgttcacaga
    12841 gatcgcattt cacaatttgt tgtcgggtat cccccggcaa cgactgaatg gtgatcattc
    12901 caaacgggca cgcactgacg caactctgac agccaataca acgggcagaa ttggtctgca
    12961 ccacctgctc gcccatcgtc agcgccccca cggggcaagc gccaacacaa ggggcgtttt
    13021 cacactgatg gcacatcacc ggcgcactga tgctgtccag tcgctgtacc ttcagccggg
    13081 gtaaaaagac atcggcattc aactcctgtt ctgaagggtg agcgaccaca caggctactt
    13141 cacaagtacg gcagccaata cagtcagccg gattaacgat aattaacgat ttcatcacga
    13201 tgccttcgta tcaaacagag ttaacatatc gcgcgccgcc tgccttcctg cggccattgc
    13261 agtgacaacc agatccgcgc catgaactgc atcaccacca gcaaagactt ttttcagatg
    13321 cgtctgggta ggtaaatacc cgacgtcacc ggtttgaatc aggccccatt tatcgagttt
    13381 aattccgctg ccctgcaacc acggcatggc atgcgcctgg aaaccaaagg ccataatcag
    13441 aacatcggcg ggcaattcaa actctgaacc cgctaccgga cgaggacgac ggcgaccatc
    13501 cggccccggc tcacccatgg cggtacgaat caggcccacc gcagttaaac gcccatcttc
    13561 gtcacaagcg atatattgcg gttgaacatt gaactgaaac tcgacacctt cctcgcgcgc
    13621 attaaccacc tctttgcgcg agcccggcat actgacttca tcacgacgat acgcgcaggt
    13681 cacgctggcg gcattgaggc ggatggaagt ccgcaaacaa tccattgtcg tatcgccacc
    13741 gcccaatacc acgactcgct taccttccac gtccgtcagc ggatactctt cagactccgg
    13801 caatcccatg agctggcggg tatgggcagt caggaacggt agagcctgaa tgacaccggg
    13861 cgcatcttca tgcggcagat ctgctcgcat catcccgtaa gtccccacgc cgatgaaaac
    13921 tgcatcatat tcagaagtta aatcgctaaa ggtaatatcg cggccaattt cacagttaag
    13981 atgaaagtcg attcccattg cggtgaatat ctctcgccgc tggcttaata ccgttttatc
    14041 gagtttgaaa ggaggaatgc caaaagtcag cataccgcca atttctggat ggcgatcaaa
    14101 gacatcaacc tgaactcccg cgcgcgccag aatatcagca caccctaacc ctgctggccc
    14161 agcgccaatc accgccactt tttcgctacg gggaacaact ttgctgacat cgggacgcca
    14221 gcccatcgcc agcgcggtat cggtgatgta gcgttccaga ttaccgatag agactgcgcc
    14281 cgagtgatct ttcaaagtac atgcgccttc acaaagacgg tcctgtggac ataccctgcc
    14341 gcaaatttct ggtaaggaac tggtctggtg gcaaagttct gccgcttcaa taatctttcc
    14401 ttcctgtacc agacggatgt aatccggaat agcgttatgc agcgggcaat gccagttgca
    14461 gttagctttt tcggcacaat aaacacagcg gtcactctca taagtcgctt gcagtggatc
    14521 cagcccgcaa tatatttcgc caaagtgggt tttccgttca ctcgctgaaa ttttatctgc
    14581 gcctttacgc gagttaacgg ggagcaacgc tgcactgcga gatggctgag catctgatga
    14641 cgcttttcct gctgccgttt tgcgctggcg agccaccttt atccactgta accctttatc
    14701 gtccatcagt cgtaacgcct gtgttgggca gacttcaata caggcttgcg tgccggaact
    14761 gcgctggtta caaaggtcgc atttctgcgc aatcgtatcg accatctcaa cgacgccaaa
    14821 ggggcaagcg attgcgcatc ttttacagcc aatacatttt tgctcgtcca gttgtacgct
    14881 atcggactgg aaagtcagag cattaaccgg acaagccgta acgcaagggg cattgttgca
    14941 gtgatggcag gccaccggat tcgcagcctg gcctttccct acaacgtgga tacgcggtcg
    15001 aaagtcactg tgactcagcg gccagttttc ttgattgtgt gccaccgcac aggcaatttc
    15061 acaagcatga cagcctatac attccgcagc ttcagcagcg ataaacttat tcatttgcat
    15121 ccctttcatt taatgagtta tgtcttctca aatttcgcga tgcaataaac gggcatatat
    15181 taggtagcat gaccgtttta attataattt tgcgactgag ttcaaattat tgcccctcaa
    15241 tctatgccgc tttcattatt tcaacattgc cgaccaaaag agcgctatcc agataaaaac
    15301 gattaaatgc gagagtgcga catgccagaa tgattaatga aatatcaata tgagaaaatt
    15361 acagataatt tcattgtcag gttctcttct cattttcgtg gtttaaccac gattatgtga
    15421 ttgcctccgc aattcctgtc tctaactccc cttcctcgca aaaactggca ctccacgagc
    15481 atgtgtttag acagtttcat taacgtaaac ggttgctttt tactctggcg ggcgaaagga
    15541 gaaacactga tgagcgccat agattcccaa cttccctcat cttctgggca agaccgccca
    15601 actgatgagg ttgaccgcat attatcacca ggaaagctga tcatactcgg tctgcaacac
    15661 gtccttgtca tgtacgcagg tgcagtcgct gttcctctta tgattggtga ccgactgggc
    15721 ctctcaaaag aagctattgc gatgctcatt agctcggatc tcttttgctg cgggatcgtc
    15781 acattattgc aatgtatcgg tatcggccgc tttatgggga tccgcctgcc ggtgattatg
    15841 tcggtgacct ttgctgctgt aacaccaatg atagccattg ggatgaaccc ggatatcggc
    15901 ctgctgggga tatttggtgc cactatagcc gcgggtttta tcaccacatt attagcgcca
    15961 cttattggtc gcttgatgcc tttattcccg ccactggtta ccggtgtggt tattacttct
    16021 attgggctta gcatcattca ggtgggtatt gactgggccg ccggaggtaa agggaatccg
    16081 caatatggta atcccgttta tttaggtatc tcctttgccg tcttaatttt tatcttgctc
    16141 attactcgct atgcgaaagg atttatgtcc aacgtcgccg tattactggg gattgtattt
    16201 ggctttttac tttcgtggat gatgaatgaa gtcaatttat ccgggctaca tgatgcctcg
    16261 tggtttgcga ttgtcacccc gatgtcgttt ggtatgccga ttttcgatcc cgtttccatt
    16321 ctgaccatga ctgccgtgtt aatcatcgtg tttatcgagt cgatggggat gttcctggca
    16381 ctgggtgaaa tagtcggtcg taaactctct tcacacgata ttattcgcgg gctgcgtgtc
    16441 gatggcgtag ggacaatgat aggcggcacg tttaacagct tcccccacac gtcattttct
    16501 caaaacgttg gcctggttag cgtgacgcgc gttcatagcc gctgggtgtg tatttcttcg
    16561 ggaattatat taatcctgtt tggcatggtg ccaaaaatgg cggtgctggt agcctccatt
    16621 ccgcaatttg tgctgggcgg cgctgggctg gtgatgttcg gcatggtact ggcgacaggg
    16681 attcgaattc tgtcgcgctg taactacacc accaaccgtt acaacctcta tattgtggcg
    16741 atcagtctcg gcgttggcat gactccgacg ctctctcacg atttcttttc taagttaccg
    16801 gccgtactgc aaccgttgct gcatagcggc attatgctcg caacccttag cgccgttgtg
    16861 ctgaatgtct tctttaatgg ctatcagcat catgctgacc tggtgaagga atccgtctct
    16921 gataaagatt taaaagtcag gacagtacgt atgtggcttc tgatgcgcaa gctgaagaaa
    16981 aatgagcatg gagaataata tgaatttttt aatgcgcgct atattcagtc tgttgttgct
    17041 ttttactctc tctattcctg tcatttctga ctgtgttgca atggccattg aaagtcgctt
    17101 caaatatatg atgctacttt tttaaatggt ttttacctgt cggcatccgc tcaaaacggg
    17161 cggttgtcga taaacgctca cttggttaat catttcactc ttcaattatc tataatgatg
    17221 agtgatcaga attacatgtg agaaattatg caaacggaac acgtcatttt attgaatgca
    17281 cagggagttc ccacgggtac gctggaaaag tatgccgcac acacggcaga cacccgctta
    17341 catcgcgcgt tctccagttg gctgtttaat gccaaaggac aattattagt tacccgccgc
    17401 gcactgagca aaaaagcgtg gcctggcgtg tggactaact cggtttgtgg gcacccacaa
    17461 ctgggagaaa gcaacgaaga cgcggtgatc cgccgttgcc gttatgggct tggcgtggaa
    17521 attacgcctc ctgaatctat ctatcctgac tttcgctatc gcgccaccga tccgaatggc
    17581 attgtggaaa atgaagtgtg tccggtattt gccgcacgca ccaccagtgc gttacagatc
    17641 aacgatgatg aagtgatgga ttatcaatgg tgtgatttag cagatgtatt acacggtatt
    17701 gatgccacac cgtgggcgtt cagtccgtgg atggtgatgc aggcagccaa tagtgaagca
    17761 agaaaattgt tgtctgcttt cgcgcagcac aattaaaaaa ccccgacatt tgccggggtt
    17821 gtgagcataa cgtaatgctt attttaccgg acgcatcgcc gggaacagaa taacgtcgcg
    17881 gatggtatgg ctgttggtga acagcattac catacggtcg atgccaatcc ccagacctgc
    17941 tgtcggcggt aagccatgtt ccagtgcggt aacgtaatct tcatcgtaga acatcgcttc
    18001 gtcgtcacct gcgtctttcg cggcaacctg atccaggaag cgttgcgcct gatcttccgc
    18061 gtcattcagc tcgctaaagc cgttaccgat ttcacgacca ccgatgaaga actcaaagcg
    18121 gtctgtgatt tccgggttaa tgtcgttacg acgcgccagc ggagaaactt ctgccggata
    18181 ttcagtaatg aaggtcggct gaatcagatg tgcttctgcc acttcttcga agatctcggt
    18241 aacgatacgg cccagacccc agctcttctc aacgtggata ccgatagatt cagcaatcgc
    18301 tttcgcagag tcgaagttat ccagatccgc catgtcggtt tccgggcgat atttcttgat
    18361 cgcttcacgc atggtcagtt tttcgaacgg tttaccgaag tccagcgtca cgtcgccgta
    18421 ggtcacttcc gtcttaccga gaatatcctg tgccagagta cggaacagcg attcggtcag
    18481 ctcgatcaga tctttgtaat ctgcgtaagc catgtagagt tccatcatgg tgaactctgg
    18541 gttatgacgt acggaaatac cttcgttacg gaagttacgg ttgatttcga atacacgctc
    18601 gaagccaccg accaccagac gcttgaggta cagttccggc gcgatacgca ggtacatgtc
    18661 gagatccagc gcgttatggt gggtgataaa cggacgcgca gcggcaccgc ctgggatcac
    18721 ctgcatcatc ggcgtttcaa cttccataaa gccgcggttc accatgaact ggcgaatacc
    18781 agagaggatc tgcgagcgta ctttaaaggt gttacgggat tcatcgttag agatgagatc
    18841 caaataacgc tgacgatagc gcgcttcctg atcctgcaag ccgtggaatt tatccggcag
    18901 cggacgcagt gctttggtca gcagacgcag ctcggtgcag tggatagaca gttcgccggt
    18961 tttggttttg aacagtttac ctttcgcgcc gaggatgtcg ccgaggtccc attttttgaa
    19021 ctgctcgttg taaacgcctt ccggcagatc gtcacgggca acgtacagct gaatgcggcc
    19081 acccacatcc tgcagggtaa cgaaagacgc tttacccata atacggcggg tcatcatgcg
    19141 gccagcaacg gcgacttcga tgttcagcgc ttccagttct tcgttctctt taccgtcgaa
    19201 ttctgcgtgc aattggtcag aggtatgatc gcgacggaaa tcgttcggga aggcaacccc
    19261 ctgctcgcgc aggttcgcca gcttctcacg acgcgttttc agttcattgt taagatcgac
    19321 taccgcgtca gcgccctgtg cgtgttgttc agacatgttg gttcctcata accctgcttt
    19381 caaacttgct tcgataaatt gatccaggct gccgtccagc acggcctgcg tgttgcgggt
    19441 ttctaccccg gtacgcagat ctttaatgcg ggagtcatca aggacataag aacgaatctg
    19501 gctgccccag ccgatgtcgg atttgttatc ttccatcgcc tgtttctcgg catttttctt
    19561 ctgcatctcc agttcataaa gcttcgcttt catctgcttc atggcctggt ctttgttctt
    19621 gtgctgggaa cggtcgttct ggcattgggt cacgatcccg gttgggatgt gggtaatacg
    19681 caccgcagat tcggtacggt taacgtgctg accgcccgcg ccggacgcgc gataaacgtc
    19741 aatgcgcaga tccgccgggt tgatttcgat atcaatatca tcatcaactt ccgggtaaac
    19801 aaacgcagag ctgaacgatg tgtggcgacg accgccggag tcaaacgggc ttttacgcac
    19861 caggcggtga acgccagttt ctgtacgcag ccagccgtaa gcgtaatcgc cggagatttt
    19921 gatcgtcacg gatttaatac ccgccacttc accttccgac tcttcgatga tttcagtttt
    19981 gaaaccacgc gattcagccc agcgcagata catacgctca agcatgctcg cccagtcctg
    20041 tgcttccgta ccgccagacc ccgcctgaat atcgaggtag cagtcggcgc tgtcatattc
    20101 gccagagaac atacggcgga actcaagctg cgccagtttt tcttccaggg cgtcgagttc
    20161 agcaacggct tcgttaaagg tttcttcgtc gtcagcttct acagccagtt ccagcagacc
    20221 agaaacatct tccagcccct gtttcatttg gtcgagggtg tcgacaacgg cttcgaggga
    20281 ggaacgctct ttacccagcg cctgtgcgcg ttcgggttcg ttccagacat ccggctgttc
    20341 cagctcggcg tttacttctt ccagacgctc tttcttggcg tcgtagtcaa agataccccc
    20401 taagaacgtc ggagcgttcc gtgaggtcct gaatgcgatt atttaccgga ttaatttcaa
    20461 acatggtctg atttctttta ttgagctagt caaaatgcgg tgataagagc gggattgtac
    20521 ccaatccacg ctctttttta tagagaagat gacgctaaat tggccagata ttgtcgatga
    20581 taatttgcag gctgcggttg ccgcgaaact cgttgatatc gagcttataa gccagttgca
    20641 cttcacgcac gccgttatcc ggccagaggg cggtatcgac attaaaagca ataccatcca
    20701 gcagtggacc gccgccgacc ggttcgacca tcaccttcaa atgacgttcg cccaccagcc
    20761 gctgttgcag cagacggaaa tgaccgtcaa acagcggctc cgggaacatc tgcccccacg
    20821 ggccagcatc gcgcagcaac tgcgccactt ccatggtcat ttcggccggg cttaacggac
    20881 cgtctgacac cacttcgcct tgcaatagcg aagggtccag ccactctgta accagttcgc
    20941 caaaccgttg ttgaaagagt tcgaatttat cctcttccag cgacaaaccc gccgccatcg
    21001 catgaccgcc aaacttcagt atcatgccag ggtagagtgt gtctaatcgc tccagtgcat
    21061 cacgcatatg cagcccctga atggagcgac ctgaaccttt cagcgtacca tcacctgctg
    21121 gcgcaaaggc gataaccgga cggtgaaaac gctctttgat gcgcgaagcc agaataccga
    21181 caacgccctg atgccattcg gggtgataca ttgccagccc gccgggtagc gtgtcgcgac
    21241 ttcgctccag tttctcgcac agggtcaggg cttcaacttg cattccttgt tcgatctctt
    21301 ttcgcgtctg gtttagcgca tcgagttcat ttgccagcac gcgcgcttcg ccgatgttgt
    21361 cgcacagcaa cagcgccaca ccgacggaca tatcgtccag tcgcccggca gcattgagac
    21421 gtggccccag cgcaaaacct aaatcgctgg cggcgagttt ttgcgcatcg cgatttgcca
    21481 cttccagcag cgctttaatc cccggacggc actttccggc acggatgcga ctcatcccct
    21541 gccaggtcag aatgcgatta ttagcgtcca gcggcacgac gtccgccact gttcccagcg
    21601 cgaccagatc cagcagttct gccaggttag gaattgcgat gccacgctca tcaaaccagc
    21661 cctgatcgcg caaaaaggtg cgcagcgcca gcatcagata aaacgccaca cccacgcctg
    21721 ccagtgattt cgacgggaaa ttacagtcgc gcaagttagg gttaatgatc gcttccgccg
    21781 cgggtaatgt ttcgcctggc aaatggtgat cggtaacaat aaccggaatg cccaacgagc
    21841 gagcgtgtac aacccccgca tgggaggaaa taccgttatc caccgtgaca attaactgcg
    21901 cgccacgggc atgggcctga tcgaccactt ccgggcttaa gccgtaaccg tcttcgaaac
    21961 ggtttggtac caggtagtcg atattgctgc aaccaagcga gcgcatcgcc agcacgctta
    22021 gagccgtgct ggtcgcgccg tcggcgtcga aatcaccgac cacaataatc cgcgttcctt
    22081 cgcgaaaagc gttgtaaagg atctcaacgg ccttttcgac gccgctcagt tgctgccagg
    22141 gcaacatacc tttaacactg cgttccagtt cttgcgcact gcgcacgccc cggctggcgt
    22201 ataaacggcg cagcaaggga ggcaattcag cgggcaagtc tgccgtttca tcgacttcac
    22261 ggcgacgaag ttgtatctgt tgtttcacgc gaattattta ccgctggtca ttttttggtg
    22321 ttcgtcgagg aattctttca tctctttcgg cggctggtaa cccggaacaa gtgtgccatt
    22381 gctcagcaca actgccggag taccgctaac gccaagctgg acgccaagtg cgtaatggtc
    22441 ggcaatatcc acgtcgcagc tggctggtgc gacgctttta cctgccatca catcatcaaa
    22501 cgctttgttt ttatctttcg cacaccagat agctttcatt tctttctctg catcgctgtc
    22561 cagcccctgg cgcgggaaag caagataacg cacggtgatc cccagcgcgt tatagtctgc
    22621 catttgctca tgcagtttgt ggcagtaacc acaggtaata tcagtaaaca cggtgatgac
    22681 gtgtttttcc tgcggcgctt tataaacgat catctctttt tcaagcgcat tcaactgctt
    22741 taacagcatc ttattggtga cattgaccgg agccgtgccg ctaacgtcat acattggccc
    22801 ctgaatgata tgtttaccat catcggtgat gtacaacacg ccgctgttag tcagaactgt
    22861 cttcatgcca gctacgggcg cgggctgaat atcgctgctt ttgatgccca ttttggctaa
    22921 cgtttgttga attgccgcgt catcagcctg agcaaaacct gaaaacgccg ctaacaaagt
    22981 aaacaacata aaacctttct tcataaatct tcccgttctt ttcagacatc acgcccgcgg
    23041 gtgatgctgt tgatgaagtt gccgcagacg ctcggtagcg acatgcgtat aaatttgcgt
    23101 ggtggaaaga tcgctgtggc ccagtagcat ctgcaccacg cgtaaatccg caccatgatt
    23161 taataaatga gtggcaaaag cgtgacgcaa cacatgcggt gacagctttt cgctgtcgat
    23221 acccgccagc acagcataat gtttaatgcg gtgccagaag gtctgtcgcg tcatctgctg
    23281 cgcacgctgg ctgggaaaca acacgtcaat tgacacacca ttcagcagcc acggacgccc
    23341 atgttccaga taggtttcca gccagtaaac cgcctcttca cctaacggta ccaggcgctc
    23401 tttgttgcct ttaccaatga cccgcactac gccctgacgc aggctgatat cactcattgt
    23461 cagtccgacc agttcagaga cacgcagccc ggtagcatac aacacttcaa gcatggcttt
    23521 atcgcgtagc tccagtggct gatcaattaa tggtgcctgt aataaacgtt cgacctgcgc
    23581 ttcgcttaaa tcttttggca aacgctgggg caatttcggt gaagcgagat gcgcactggg
    23641 atcgtcttca cgaaactttt cgcgataaag atactggaac aatcggcgca ctgcactcag
    23701 caaacgcgct gagctggtgg ctttataccc gccctccagc cgttctgcca gtaatgcctg
    23761 caaatcgtca ctttgcgccg tcgccagcgt caacccgcgg tgatgcaacc actccaccat
    23821 cattgacaga tcgcgacggt aagcgttcaa cgtattttca gccagatttt tttccagcca
    23881 cagagcatca agaaactgct cgatgcgtgc cagatcctgt ttcacttgcg ccccttatgg
    23941 tcactcattt gatccattat gccttattgt gccgtgacta aagcgattct gatacactag
    24001 ctgcaaaagc cacagcagaa tcgagaagct tacgttatga atatgggtct tttttacggt
    24061 tccagcacct gttacaccga aatggcggca gaaaaaatcc gcgatattat cggcccagaa
    24121 ctggtgacct tacataacct caaggacgac tccccgaaat taatggagca gtacgatgtg
    24181 ctcattctgg gtatcccgac ctgggatttt ggtgaaatcc aggaagactg ggaagccgtc
    24241 tgggatcagc tcgacgacct gaaccttgaa ggtaaaattg ttgcgctgta tgggcttggc
    24301 gatcaactgg gatacggcga gtggttcctc gatgcgctcg gtatgctgca tgacaaactc
    24361 tcgaccaaag gcgtgaagtt cgtcggctac tggccaacgg aaggatatga atttaccagc
    24421 ccgaaaccgg tgattgctga cgggcaactg ttcgtgggtc tggcgctgga tgaaactaac
    24481 cagtatgacc ttagcgacga gcgtattcag agctggtgcg agcaaatcct caacgaaatg
    24541 gcagagcatt acgcctgatc tcactgacgg cttagcgcat atgctttgcc gtcatcttta
    24601 tctttgcgtc tcttgttgca acaaaatccg ccgtaaatcc cgccattcag cttcgtccat
    24661 gctgtcggct gccagccata aatgttgccg tttaccgcca tcagaacgta aacgcagcat
    24721 catgccgctc ttaatcatcc acggtgcttt gacgatgctc cactcctgcc cttgccaacg
    24781 caaacgcccg tccatcaaca agcgaatttc cccctggcga gcattaatac gccgctggct
    24841 gcgaacgcaa tcaaacacca ccagcgaaag taacaccatc cataacgggg tgtaactgag
    24901 tggccagggc atgagtaaaa taacagcggc aaccagccca tgaatcagca aggaaagcca
    24961 ctgtgcgcgc caggagacgc gcaaatcaga ttgccacagg accacgttcc cggttccgtg
    25021 tctggatgag tcggaccatc atttccagtt ctgcatcggc cggtttaccg tgattcatca
    25081 gccagttaaa caggtccaga tcgtcacatt ccagcagacg aataaagatg cgtttttcgt
    25141 catcgcttaa gctgtcgtac tcatgttcga aaaacggcat gattgaaata tcgagttcgc
    25201 gcataccacg gcggcatgcc caatgaatgc gggctttgtt gttaatgtcc atcttcttcc
    25261 tgtctcacga aaatccagta ccccgctatt gtaacgtgtt tttcgccttc ttttacggga
    25321 atatcagtaa acaccatcgc gatcgcgaaa ttaattcaca acaattcaat ggcttcattt
    25381 ttttggaagt cgcctcgcag aaggcacaga tcgcgtagtg aaagcacttg cattgcctca
    25441 tagctctttt accattagtc attaatacgc cgttaagcaa ctcaggactc tattatggct
    25501 tttacacctt ttcctccccg tcagcctacg gcttctgccc gtttaccgct gacgctgatg
    25561 acgcttgatg actgggcgct tgccaccatt actggcgcgg acagcgaaaa atatatgcag
    25621 ggtcaggtga cagcagatgt cagccagatg acagaaaatc agcacctgct cgccgcccat
    25681 tgcgacgcca aaggtaaaat gtggagcaat ttacgtctgt tccgcgacgg cgatggcttt
    25741 gcatggattg aacggcgcag cgtgcgtgaa ccgcagctga ctgaactgaa aaaatatgcg
    25801 gtattctcta aagtgaccat cgcgccagac gacgagcgtg tgctgcttgg tgttgccggt
    25861 tttcaggcgc gcgccgcgct ggcaaatctc tttagcgaac tgccttcgaa agaaaaacag
    25921 gtagtcaaag aaggcgcgac cactttgcta tggtttgaac acccggcaga acgtttcctg
    25981 atcgtaaccg atgaagctac tgctaatatg ctgaccgata aactgcgcgg tgaagcggaa
    26041 ctgaacaata gccaacagtg gctggcatta aacattgaag cgggtttccc ggtgattgat
    26101 gccgccaaca gcgggcagtt tatcccacag gcgaccaatc tccaggcgct gggcggtatc
    26161 agctttaaga aaggctgtta taccggacaa gagatggtgg cgcgagcaaa attccgtggt
    26221 gccaataaac gtgcgctctg gttgctggca ggtagcgcca gccgactgcc ggaagctggt
    26281 gaagacttag agctgaaaat gggcgagaac tggcgtcgta ccggtacggt gctggctgcg
    26341 gtaaaactgg aagatggtca ggtcgtggta caggtcgtca tgaataacga tatggaaccg
    26401 gatagcatct tccgcgtacg cgacgatgcg aatacattgc atatcgagcc gctgccgtat
    26461 tcgctcgaag agtaaatctc tttatcgcat caggcattta tcgcctgatg cgacgctgac
    26521 gcgtcttatc atgtctggga tttgatgcct ttatcacaac agggacgagg cccgtagatc
    26581 ggataaggcg ttcacgccga atccggcagt tgtgctccga tgcctgatgc gacgctggcg
    26641 cgtcttatca ggcctacaaa ggcataccca ttacgcctgc ccaatataca aatagatcgc
    26701 cagaaagtgg cacacactac cgccgagcac gaagccgtgc cagatggcat ggttgtatgg
    26761 aatgcgtttg cagacgtaga aaatcacccc gagcgaataa accacgccgc ctaccgccag
    26821 taaggtaacg ctgcccgccg cgagcttaac tgccatttca taaattacca ccagcgacag
    26881 ccagcccatc gccagatagg tcaccagaga taaaattttg aatcggtgcg cgatggtcag
    26941 tttaaacaga atacccagca atgccaggct ccagataaca atcatcaacc cgcgcgctaa
    27001 cggagaatcc agccccacca gcaaaaacgg cgtgtaggtt ccggcaatca acaggtaaat
    27061 agcgcaatgg tcaaatttct tcagccacat ttttgcccgt tgatgaggaa tggcgtgata
    27121 gagcgtcgaa gcgaggaaca gcaggatcat actgccgcca tagaggctgt aactggttat
    27181 cgcagtggcg ctggcattga gatccaccgc ctgaaccagt agcaacacca gcccaacgat
    27241 accaaacacc aacccaatgc cgtgactgac gctgttggca atttcctctg ccagcgaata
    27301 tccctgctta atgaggggct tctgaaccat aacttactcc ggagaaacgt acacgcacat
    27361 gtatacctct ccagcgtaac tgagaatagt tccagtgaac acctgttagc taaaataaat
    27421 tctgatttga gactaatctc ctaaaaatca tgaaattaaa tgcgaaattt caactaacag
    27481 gcgcgagttc aatttaaaga catttaaatt caatcacata aaactgtgtc tgaccgggat
    27541 agatgtcggc aatgaccttt atcagttcag tcagggtcat attttcctgc tctgcatgtt
    27601 tttcagtcag cgtatccagc gttacggttg aggttgcggt gacttcaatc gtgcaaaaat
    27661 aaccgtcatc ttcaaaacgt ccgacacgaa gcacatcacc cgttttgaag tgcgattcag
    27721 actcgtcgcg gatggtgatg gttttacgcc cagccagaat gtcatcctgg aaacgttgaa
    27781 aaaaagtgat gtcgtttggc tgcatggtac tatttcctgt aagaattgac tcatctggag
    27841 cctatgatag tgaaaaaact caccttaccg aaagatttct tatggggcgg cgcagttgcc
    27901 gctcatcagg tcgaaggcgg ctggaacaaa ggcggaaaag ggccgagcat ttgtgacgtt
    27961 ctgaccggtg gcgcacacgg cgtgccgcgc gaaatcacca aagaagtctt gccaggcaaa
    28021 tactatccaa accatgaagc cgttgatttt tatggtcact acaaggaaga catcaagcta
    28081 tttgccgaaa tgggcttcaa atgttttcgg acttccatcg cctggacgcg tatttttcca
    28141 aaaggtgatg aagctcagcc aaacgaagaa gggctgaagt tctacgatga tatgttcgat
    28201 gaactgctga aatacaacat cgaaccggtg atcaccctct cccactttga aatgccgctg
    28261 catctggtgc agcaatacgg tagctggacc aaccgtaaag tggttgattt ctttgtccgt
    28321 ttcgcggaag tggtgtttga acgctataag cataaagtca aatactggat gaccttcaac
    28381 gaaattaaca accagcgtaa ctggcgtgca ccgctgttcg gttactgctg ctccggcgtg
    28441 gtttataccg agcatgagaa cccggaagag acgatgtatc aggtgctgca tcaccagttt
    28501 gtcgccagcg ccctggcggt gaaagctgcg cgtcgcattc acccggagat gaaagtcggc
    28561 tgtatgctgg cgatggtgcc gctctatccc tactgctgta acccggacga tgtgatgttc
    28621 gctcaggagt cgatgcgcga acgctacgtc tttaccgatg tgcagctacg cggctattac
    28681 ccgtcctatg tgttgaacga gtgggagcgt cgcggattta acatcaaaat ggaagacggc
    28741 gatctcgatg tactgcgtga aggcacctgc gattatcttg gtttcagcta ttacatgacc
    28801 aacgcagtga aggccgaagg cggcaccggc gatgcgatct ctggtttcga aggcagcgta
    28861 ccgaacccgt atgttaaagc gtctgactgg ggctggcaga ttgatccagt aggtctgcgc
    28921 tatgcccttt gcgaactgta tgagcgttat cagaggccgc tgtttattgt cgaaaacggt
    28981 tttggcgctt acgacaaagt ggaagaagat ggcagcatca acgacgacta ccgcattgac
    29041 tacctgcgcg cccatatcga agagatgaaa aaagcggtga cttacgatgg cgtggatctg
    29101 atgggctaca caccgtgggg ctgcatcgac tgcgtatcgt tcaccaccgg gcagtacagc
    29161 aaacgctacg gctttatcta tgtgaataaa catgacgacg gtgctggcga tatgtcgcgt
    29221 tcacgtaaga agagctttaa ctggtaccaa gaggtgattg ccagcaacgg cgagaagctt
    29281 taagtcgatg aagtaccgga tgcaatactt gttgcatccg gtcagacaac ttccctgttt
    29341 tatttcccgc ccgccaaatc gataaaactt cccgtgacgt aagaggcttt atcacttagt
    29401 agccagacaa tggcctgcgc gacctcttct gcctgtccac cacgctgcat ggggatgttc
    29461 gacttaacgc gatcgacgcg tccaggctcg ccgccgctgg cgtgcatttc ggtataaata
    29521 aaccctggcc gcacgcagtt aacgcggatc ccctgcgcgg cgacttccag cgatagtccg
    29581 gtggttaacg tatcaatcgc ccctttcgat gccgcgtaat caacatattc ccctggcgaa
    29641 cccaaccgtg aggccaccga agagacattg acgatagcgc cgccactgcc accattttta
    29701 agcgccatgc gttttaccgc ctcgcggcag cagagaaaat atcccgtcac gttggtggaa
    29761 agtactcggt tgattcgctc tgcggtaagg ttttcaacgg tgcactgggt aaacaagatc
    29821 ccggcgttat tgaccagcgc tgctagcggt tcatcgtgct gatcgattgc tgtaaacatc
    29881 gcaacgacct ggttttcgtc gctgatatcc gcctggagca cgaatgcttt gccaccggct
    29941 tgcgttatta agttcatcac ttcctgcgcc gcgtggaggt tttgctgata attaaccgcc
    30001 accgtatacc cttcttgcgc caacagtaat gcagttgccc gcccgatgcc gcgactgcca
    30061 ccggtcacaa gtgctatagc catttgtttt tccgagaaaa attcattcgt tgacgtatct
    30121 ttgagattgc taacaaactg gctcaatctt caatgttggc acgcttttcc ccctcgccct
    30181 ttcagggaga gggccggggt gagggtaaat attcgcgcca gtgccggcct gttcccctca
    30241 ccctctcccc aaaggggcga ggggactgtc tgagcacttt tgtactttgt catctgacta
    30301 aaaaggcgcc gaagcgcctt tagaaaatag tcgaatcagt gaattactgg tattcgctaa
    30361 tcggtacgca ggagcagaac aggttacggt cgccgtaaac atcatccaga cgtttcaccg
    30421 tcggccagta tttgtctgcc acacctgccg ggaatactgc aacctcacgg ctgtacggat
    30481 gcgcccactc ggcgaccagt tcgttctgaa tgtgcggcgc gttcaccagc gggttatctt
    30541 ccagcggcca gacaccggct ttaacctggt cgatttctgc gcggatagcc agcatcgcgt
    30601 cgataaagcg atccagttcc actttgcttt cagattcagt cggttcaacc atcagcgtac
    30661 ccgccaccgg gaacgacatg gtcggcgcgt ggaaaccgta gtcgatcagg cgcttggcaa
    30721 tatccagctc gctgatgccg gtttcttctt tcagcgggcg aatatcgaga atacattcgt
    30781 gcgccacgcg accgtcgcga ccggtataca gcaccgggaa ggcatcctgc aggcggctgg
    30841 caatatagtt ggcgttgagg attgccacct ggcttgcttt tttcagccct tctgcgccca
    30901 tcatgcggat gtacatccag ctgattggca ggatagaggc gctaccgaac ggtgccgcag
    30961 aaactgcgcc ctgacgggtt aacatgcctt cgatttgcac cacgctatga cccggtacaa
    31021 acggtgccag atgcgctttc acaccgatcg gtcccatacc cggaccacca ccgccgtgcg
    31081 gaatgcagaa agttttatgc aggttgaggt gcgaaacgtc cgcgccaata aagcccggcg
    31141 aggtgatgcc aacctgggcg ttcatgttcg cgccatcaag gtaaacctga ccgccgaact
    31201 gatgcacgac ttcacacact tcacggatcg tttcttcata cacgccgtgg gtagaaggat
    31261 aagtcaccat gatacaggag aggttatcgc ccgcctgttc cgctttcgcg cgcagatcag
    31321 tcagatcgat gttgccgttt ttatcacacg ccacaaccac cacctgcatt cctgccatat
    31381 gtgcagaagc ggggttagtt ccgtgcgcag aagccgggat caggcagata tcgcgatgcc
    31441 cttcgttgcg gctttcatga taatgacgaa tcgccagcag gcccgcgtat tcgccctgtg
    31501 cgccggagtt cggctgcata caaacggcgt cgtaaccggt cagtttcacc agccagtcag
    31561 ccagctgcgc aatcatctgc tgataacctt cagcctgttc cggcggacaa aacgggtgca
    31621 gttcggcaaa ttccggccag gtgattggga tcatctcggc ggcggcgttc agtttcatgg
    31681 tgcaggaacc cagcgggatc atcgcctgat tcagcgccag atctttacgc tccagcgagt
    31741 gcatatagcg catcatttcg gtttcgctgt ggtagcgatt aaacaccgga tgggtgagga
    31801 tttcgtcgtc gcgcagcatc gcaggctgga tagagcggct gtcgtgagcc acgtctttgt
    31861 ccagcgtgtc gatgtccagg ccgtggttat cgcccagtag tacgctgaaa agctgcatta
    31921 cgttttcacg cgtggttgtt tcatcaaggg tgatcccaac cgcgttcaga atatcgctac
    31981 gcaggttgat ttcagccgct tcggcacgcg tcagtacgcc cgctttgtcg gccacttcca
    32041 cacacaaggt gtcgaaatag tgcgcatggc gcagtttcag acctttttgt tgcaggcccg
    32101 ccgccaggat atcggtcaga cggtgaatgc ggttagcgat acgtttcagg ccaatcgggc
    32161 cgtgataaac ggcatacagg ctggcgatgt ttgccagcag cacctgggaa gtacaaatgt
    32221 tggagttcgc tttctcacgg cgaatatgtt gctcgcgagt ctgcatcgcc atgcgcagcg
    32281 cggtattgcc agctgcatct ttcgatacac cgataatacg gcccggcatt gagcgtttgt
    32341 attcatcttt cgccgcaaag aaggccgcgt gtgggccacc gtagcccatc ggcacgccga
    32401 agcgttgcgc cgaaccaaaa acaatatccg cgccctgttt acccggcgca gttaacagca
    32461 ccagcgccat aatatcggcg gcgacactga ccacaatttt gcgtgatttc agttcgctaa
    32521 taagcgcggt gtagtcgtgg atttcaccgg ttgtgccaac ctgctgtaac agcacgccga
    32581 agacgtcctg atggtcgagc actttttgcg cgtcatcgac aatcacttca aaaccaaagg
    32641 tttcggcacg agtacggacc acatccagcg tttgcggatg cacatcggaa gccacgaaga
    32701 agcggttggc atttttcagt ttgctgacgc gtttcgccat cgccattgct tcggcggcag
    32761 cggtggcctc gtccagaaga gaagcagagg ccatatccag tccagtcaaa tccagcgtta
    32821 cctgctggaa gttgagcagt gcttcaaggc ggccctggga gacttcaggt tgatacggag
    32881 tgtacgcggt ataccagccc ggattttcca gcatgttacg caggataacc ggcggtagct
    32941 gcacggcggt gtaacccatg ccgatgtaag acgtgaagcg tttattgcga ctggcaatag
    33001 ccttgagttc tgccagtgcg gcgtattcgg tcgccggtgc gccaacctgc ggcggggtcg
    33061 caagctgaat atctttcggc acaatctggc cggtcagcgc gtttaacgat tgtgcaccaa
    33121 cggcattcag catttcttgc tgttgcgcgg cgtccggtcc gatatggcgt tcaataaaag
    33181 cgccgctgtt ttcaagctgg cttaacgtct gtgtcatgag cgatggttcc tgaaacgtgc
    33241 agtgaattgt gaacctctct ccttacgaag agagtgaggg tgaggcataa attttcctca
    33301 ccctgatcct ctcccgcaga agaggaataa agccgttact cgtcttctaa caatgcttcg
    33361 tatgcggtcg catccagcag tgattccagt tcgctttcat cgctggcttt gattttaaag
    33421 atccagccac ctgcatacgg ttcgctgttc accagttccg gggaatcgct cagtgcgtcg
    33481 tttaccgcca cgatttcacc gcttactggc gcataaatgt ctgacgccgc ttttactgat
    33541 tcggcaaccg cgcagtcatc gcccgcgcta accgttgcgc ccacttccgg caggtcaaca
    33601 aacaccatat cgcctaacag ctcctgggca tgttcggtta taccaacggt gtaagtgccg
    33661 tcggcttctt tacgcagcca ttcgtgttct ttgctgtatt tcagttctgc tggtacgttg
    33721 ctcatcaatc aatctccaaa aaagtaaatc acgcgacggc tttaccgtta cgcacaaaaa
    33781 caggtttcgt cactttaacc ggcatttcac ggttgcgaat ttgcacaatc gccgtttcgc
    33841 caataccttc cggcacgcgt gccagcgcaa tgctgtaacc cagcgtcggg gagaaagtcc
    33901 cgctggtgat aatgccttca tgctggttgc cctgcgcatc ggtaaaacgt accggcagtt
    33961 cattacgcag cacgcctttt tcggtcatca ccagaccaac cagtttttct gtgccatgct
    34021 cacgctgcac ttccagggct tcacgaccga tgaagtcacg atctgccggt tcccaggcga
    34081 tagtccagcc catgttggcg gctaaaggag aaatagtttc gtccatctcc tgaccataaa
    34141 gattcatgcc cgcttccaga cgcagcgtgt cacgcgcgcc cagaccgcat ggcttaacgc
    34201 ccgcttccac cagcgcacgc cagaaatcgg ccgctttttc attgggcagc gcaatttcat
    34261 agcccgcttc accggtataa ccagtggtgg caataaacag atcgcccgcc tgcacgccaa
    34321 agaacggttt catcccttcc accgcctgac gctgagcatc attaaacagt gtggcagctt
    34381 ttgcctgcgc attcggccct tgtacggcga tcatggaaag gtcatcacga acggtaattt
    34441 cgatgccgaa aggttcagcg tgttgggtaa tccaggagag gtctttttcg cgggtggcgg
    34501 agttaacaac gaggcggaag aaatcttcag taaagtagta gacgatgagg tcatctatca
    34561 caccgccaga ggcattcaac atccccgagt aaagggcttt gccgcttttg gtgagcttcg
    34621 ccacatcgtt cgccagcaga taacgcagaa actcccgggt gcggctgccg cgaagatcga
    34681 cgatggtcat atgtgacaca tcaaacattc cggcatcggt acgtaccgca tgatgttcgt
    34741 cgatttgcga accgtaatgc agcggcatca tccagccgtg gaaatccacc atgcgagcgc
    34801 cgcaaagcgt gtgttgttcg tacaaaggag tctgttgtgc catcttgtcc tcattgaata
    34861 agcggggctg acaacttttt catggtgaaa ttatcaccac gaaacccagc atcggagcca
    34921 ctcccggtcc ccaacgcaat cgttctcttt tgcctgaact taccaccgaa acagactgtt
    34981 aaccataagg taaaattgat catcacatta gcttatggtt aaaaaacgcc aaaatcgcaa
    35041 cagaataaaa aaccaaaaaa tacacaagtt tttatataaa gatgatgtga tgagaaagtt
    35101 aatttgaata aggcaatatt aagagctgaa aaaatgtcaa aaaacactaa atcaaaaatt
    35161 cgtgacatta gaaaatataa tgcgaaaacg gaggtgaaat tagtttattt caaatgagga
    35221 aaatctcccg gcgaaaaaac cgggagatga aagtgtgatg ggtatcaaat aaacagcaga
    35281 agagaaattt ttaacgcagc cattcaggca aatcgtttaa tcccattgcc tggcggataa
    35341 gttgtggttt aacgccagga agcgtgtcgg ccagtttcaa accaatatca cgcagcagtt
    35401 ttttcgccgg attggtaccg gaaaacagat cgcggaatcc ctgcatacca gccagcatca
    35461 gcgccgcact gtgcttgcgg ctacgctcat accggcgcag gtaaatgtac tgcccgatgt
    35521 ctttcccctg acgatgcaac cgtttcagtt cagcaatcag ctctgcagca tccataaagc
    35581 cgagatttac tccctgcccc gccagcgggt gaatggtatg cgcggcgtcg cccaccagca
    35641 ccagacggtg cgcggcaaac tggcgcgcat aacgccccgt cagtgggaac acctgacgcg
    35701 cgctctcaac cttgcataag cccaggcgat tatcaaaagc gatatttaac gcgcgattaa
    35761 attcatcttc acttgcctgc tgcatccgct gcgcttcctc tggcgacagt gaccagacaa
    35821 ttgagcaaag atgcggatcg ctaagcggta aaaaggccag aatgccttcg ccatggaaaa
    35881 cctgccgcgc caccgcatca tgcggttctt ccgtgcgaat ggtcgctacc agcgcgtgat
    35941 gctgataatc ccagaaagtc agcggaatat ctgctttgtt gcacaaccag gaattagcac
    36001 cgtccgcgcc aatcaccaga cgcgccgtta acatgctgcc atctttcagc gtcaggaaag
    36061 tttcattttc tccccaggcg acctgctgta attctgcggg ggccaacaga gtgatatctg
    36121 acgactgctg cgctttgttc cacagcgcgt agtgaatcac tgagttttca acgatatgcc
    36181 caagatggct atagcccatg ctttgatcgt caaacgaaat gtgaccaaaa ctgtctttgt
    36241 cccacacttc cataccgtga taacaactgg ccctacgaga gagaatgtcc tgccagacgc
    36301 caagacgggt gagtaatttt tcgctggcgg cattgatagc cgaaacgcgc agttgtggtg
    36361 gtgcatccgc cgccagaggt tccggtacgc gctgctccag tacggcaacg cgtaagccgc
    36421 tcccctgtaa gccgcaggca accgccagcc ccaccatgcc gccaccaaca atggctacat
    36481 caacactttg cattgtttat tccttaaaac cgccttcaac gcgccaccca accgagggtg
    36541 cgctgcgcca gcacatcgcg tgccggggtg aataattcca tcgtcatcag tccgatgttg
    36601 cgcccgacaa ccagcggtgc ccaacggttg gcaaaaagat gtaccaggct gtccgtgacg
    36661 ccgatggttg cttcgcgatc actctgtcga cgctgctgat agcggcacaa tatgccgtaa
    36721 tcaccgatgt cttctccgcg ctcatgcgcc tgagtcaggg tttccgcaag gctcatgaca
    36781 tctcgcatac cgaggttaaa cccttgcccg gcaatcgggt gcagagtttg cgccgcattg
    36841 cccaccagca cggtacgatg ggtaatagct ctggcggcgc gggttaacgc cagcggataa
    36901 gcactgcgtt taccggcgtg ggtaattttt ccaagtcgcc agccaaaggc cgactggagt
    36961 tcacggcaaa acttctcgtc gctccacgac agcacctctt cgcgccgttc cagtggatga
    37021 caccagacca gcgaacagcg tccgtcagac atcggcaaca tcgccagcgg gccatgttgc
    37081 gtgaagcgtt caaaagcgcg gccttcgtgc gcaacggaag tcgcaacgtt ggcaatcacg
    37141 gccagttgtt cgtaaggctc ctgctgccag tcaacgccgc acgcggtggc taacgctgaa
    37201 tgggtgccat cagctgctac cagcacgcgg cccgtcagcg tctcgccact ctccagcgtc
    37261 acttcaacgt gactctgagt acgggcaacg ttagccacgc gatcagggca atgcagcgtt
    37321 acgccaggtg ctttacgcag caatgcaaac agccgttgcc cgacattgtg caattcgaca
    37381 acctgtccca gcgccgccag ttggtaatct tctgcggcga gggtgacaaa tccagcgtga
    37441 ccacgatcgc tgacatgcac ggtagtgatg gcggttgcgt aatccgccag agattgccag
    37501 acgccgatgc gcgccagttg ctgacaggtc cccgcagcca gcgcaatagc tcgtccatca
    37561 aagcccggat gagcatgtga ctctggcgcg gtcgcttcaa tcaaatgtac cggcagcgcc
    37621 ccgtgactta accgggaaat agccagcgcc agcgtcgcgc ccgccatgcc gccaccgacg
    37681 atgattacgc tcattgcttt ctcgcagctg ccatcaacgc ttcgatttct tccggctttt
    37741 tcaccacgct ggcggtgagg ttttcgttgc cggtttcggt aatcacaatg tcgtcttcaa
    37801 tacgaatgcc gataccgcga tattgttctg gcacatccgc atccggcgcg atatacagac
    37861 caggctctac ggtcagtacc atccccggtt ccagaatgcg cgagcgatcc tgaccataaa
    37921 cgccaacgtc atggacatct agccctaacc agtggctaag gccatgcata aagaaaggac
    37981 gatgggcgtt ctgagcgatc agttcatcaa catcaccttt cagaatgccg agttttacca
    38041 ggccgctaac catgatgcgc accacttcac cagtgacttc cagaatggaa gttcccggac
    38101 gatacaggcg caagctggtt tcgagagact ccagcacaat gtcgtagatt tcacgctggg
    38161 cctgagtgaa tttgccgttg accgggaagg tgcgggtaat atcgccagcg taacctttgt
    38221 attcgcaacc cgcgtcaatc aacaccaggt cgccgtcgcg catttcacac tcgttttcgg
    38281 tgtagtgcag aatgcagccg ttttcaccgc tgccgacaat ggtgttatag gacggatagc
    38341 gcgcaccgtg gcggttaaat tcgtggtgaa tttcgccttc cagatggtac tcgaacattc
    38401 ccggacggca tttttccatc gcccgggtat gtgccatggc ggtgatttct cccgcgcggc
    38461 ggagtacggc aatctcttct ggcgatttga acaggcgcat ttcatgaaca acaggacgcc
    38521 agtcgatcat cgttgccggt gcggtgagat tttgccgcga acctttacgc agtttttcca
    38581 gcgcactgtt cacgatttca tcagcatatg catattcgcc ctgggcatgg taaaccacat
    38641 ccaggccgtt aagtagttga taaagttgct gattgatttc gctgaatgcc agtgcgcggt
    38701 caacgcccag tttctctggc gcggcatcct ggcctaagcg acggccaaac cagatctccg
    38761 ccgtcaggtc gcgaacgcgg ttaaacagaa cgctgtggtt atgagtgtca tcgcttttaa
    38821 tcagcaccag caccgcttcc ggttcgttaa agccggtgaa gtaccagaag tcactgttct
    38881 gacgataggg gtattcgctg tcggcgctac gtgttacttc tggtgcagca aaaatcagcg
    38941 cggcgctgcc gggttgcatt tgctccacca gggcctgacg gcgacgctga aactcttgcc
    39001 gggatatctc actcataaca ctctccttac gttttttgtt tttagtgtag agtcggtttt
    39061 tgtacttctg gcgcggtcgg ttgcggatga gtaaaggtgt cgtggcataa cagcgcggca
    39121 acacgaacgt attcgatgat ctcttcaagc gacatttcaa gctcttcctg atcttcgtct
    39181 tcgtcgtaac ccagttgcgc aatgttacgc agatcgtcga tagcttcacc ggtttcgccg
    39241 gtcactttat ccagcttcgg ttgcgtaacg ccaagaccaa gcaggaagtg attgacccaa
    39301 cctgccaatg catcagcccg atcgaaaacg ctgacatcat cgccatcagg cagataaagc
    39361 tgaaaaagga agccgtcatc ctgcagggca tcgctggtgg cagagtgcat tttacgcagt
    39421 gcctgtgcca gctcatgacc gaaagccatg ccttcgttcg tcaggtcgtg aagtagcggt
    39481 agccatgagc tgtcatcgtt accgccacat atcatcccac tgattaaacc atgcatctca
    39541 gcaggggtca gacccgtccc ttgttggttc agatactggt tcatttcgtt gtaaccaggc
    39601 atttcgttct gtatagacat aagcattcgt cgtcaaaggg aggaatattc atgatatgct
    39661 accactttgg gccctggtgg accagaaaag ggcttgtctc ttctcatcag ggtagctata
    39721 gtgtcgcccc ttcgcagacc atgggtctaa agacgaaggc agcgcagtca atcagcagga
    39781 aggtggcatg tctgcacaac ccgtcgatat ccaaattttt ggccgttcac tgcgtgtgaa
    39841 ctgcccgcct gaccaaaggg atgcgttgaa tcaggcagcg gacgatctga accaacggtt
    39901 gcaagatctg aaagaacgca ctagagtcac aaatactgaa cagttggtct tcattgccgc
    39961 attgaatatc agctatgagt tagcgcaaga aaaagcgaag actcgtgact acgcggcaag
    40021 tatggaacag cgtattcgga tgctgcagca gaccatagaa caagcgttac ttgaacaagg
    40081 tcgcatcacc gaaaaaacta accaaaactt tgaatgacac ttttcggttt actgtggtag
    40141 agtaaccgtg aagacaaaat ttctctgaga tgttcgcaag cgggccagtc ccctgagccg
    40201 atatttcata ccacaagaat gtggcgctcc gcggttggtg agcatgctcg gtccgtccga
    40261 gaagccttaa aactgcgacg acacattcac cttgaaccaa gggttcaagg gttacagcct
    40321 gcggcggcat ctcggagatt cccttcttat ctggcaccag ccatgacgca actaccagaa
    40381 ctcccactga cattatcccg acaagaaatc cgcaaaatga ttcggcaacg tcgtcgtgcg
    40441 ttaacgccgg aacaacagca ggaaatgggt caacaagccg ctacccggat gatgacttat
    40501 cccccggtgg tgatggcgca tacggtcgct gtattcctct cttttgatgg tgaactcgac
    40561 acccagccgc taatagaaca actctggcgc gccggtaagc gcgtatatct tccagttttg
    40621 catcccttta gtgccggtaa tttgctgttc ctgaattacc atccgcaaag cgaactggtg
    40681 atgaacaggt tgaagatcca tgagccaaaa ctggatgtgc gtgacgtgct acccctttcc
    40741 cgattagacg tgctgatcac accgctggtc gcctttgatg agtacggtca gcgcctggga
    40801 atgggcggtg gtttttatga tcggacctta caaaactggc agcactataa aacgcaaccg
    40861 gtgggttatg cgcatgattg tcagttggtg gaaaaactcc ccgttgaaga gtgggatatc
    40921 cctcttcctg cggtggttac accgtcgaaa gtctgggagt ggtaagggcg atacacctgc
    40981 atcgccctga ttgacatcgt tgattcttta acctaattta gtgagtaagg gtaagggagg
    41041 attgctcctc ccctgtgact gactgttaat aagctgggaa acttatgagt aacagtacaa
    41101 tcagtatgat ggcaagtcgc atcataaccc ttctccttca agccctcgct tcggtgaggg
    41161 ctttaccgtt acagccccat gctgcccagc tatcgtaagc tctctttaaa taaccacaac
    41221 gcattgatct acctttaaat cttttcttcg agcgtgctcg aaaaacccca tgcaaaacag
    41281 agataatttt tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat
    41341 caaaaagttc ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt
    41401 tgacctaatt tagtgagtaa gggtaaggga ggatgactcc tcccctgaga ctgactgtta
    41461 ataagctccg aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac
    41521 ccttctcctt taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc
    41581 gccatcgtaa atccccatta aataaacaca acgcattgat ctgactttga tttattttct
    41641 ggagcagact cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa
    41701 acgggcaagt cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg
    41761 ggcgcgaatg gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc
    41821 gtcagcttca atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc
    41881 ggcgatgttg acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg
    41941 gttttcgtgg atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa
    42001 gttcaccgca gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc
    42061 caggccgata ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac
    42121 gttgtcgaat tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg
    42181 gaatacgtcg attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg
    42241 aatatccacc acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga
    42301 aatctctttc gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac
    42361 cacatcgctc atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg
    42421 cagtttgttt tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc
    42481 caattgcgta ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa
    42541 agaacccgcc gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac
    42601 gccgcgcaat agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga
    42661 gaacggtgcg ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt
    42721 tgttccgata cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc
    42781 agtcagatgg gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa
    42841 ttgttcatca tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag
    42901 gctttccagc gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc
    42961 cagcgatacc tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca
    43021 tccgcctttc aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg
    43081 ttgaggaaat atcacgcaaa ggtaattctt ggatgaatat gctggtttag aggatttatt
    43141 tagagcaatc gacaattgcc tggtaaaagc gtgacacatg tcaccaaatt taatgaagag
    43201 aattttttta acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc
    43261 aggtgtgcca atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac
    43321 gccaggaatc gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg
    43381 gacgtcgagg atcacgttgc cattatcggt caccacgccc tgacggtatt ccggacgacc
    43441 gcccagtttc accagctgac gcgccactgc actacgtgcc atcgggataa cttctactgg
    43501 cagcgggaat ttacccagaa tatcaacctg cttggaagcg tctgcaatac agataaattt
    43561 ttctgcaacc gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat
    43621 ttgcatgtgg ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc
    43681 gttgagatca aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact
    43741 ggaaacggcc ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc
    43801 ggtggaacct gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca
    43861 tcctactgct tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt
    43921 cacacgcatt atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact
    43981 catccataaa atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca
    44041 acggtctggg tatgccgctc cacggtgcga acagaggcaa taacgccacg ccattcaaca
    44101 caaccagggc gcgaactcgc tgagcgaaat gcattagcgc aaatccctga tcttttaagc
    44161 tgcgcgttta accacgaata caaatgttac ccgccgacgg cttcggtata tgcaacctga
    44221 cacaaaattg tgtcatagtg caggaaaaag catttaccag gagcagacaa cagcaatgaa
    44281 acgcccggac tacagaacat tacaggcact ggatgcggtg atacgtgaac gaggatttga
    44341 gcgcgcggca caaaagctgt gcattacaca atcagccgtc tcacagcgca ttaagcaact
    44401 ggaaaatatg ttcgggcagc cgctgttggt gcgtaccgta ccgccgcgcc cgacggaaca
    44461 agggcaaaaa ctgctggcac tgctgcgcca ggtggagttg ctggaagaag agtggctggg
    44521 cgatgaacaa accggttcga ctccgctgct gctttcactg gcggtcaacg ccgacagtct
    44581 ggcgacgtgg ttgcttcctg cactggctcc tgtgttggct gattcgccta tccgcctcaa
    44641 cttgcaggta gaagatgaaa cccgcactca ggaacgtctg cgccgcggcg aagtggtcgg
    44701 cgcggtgagt attcaacatc aggcgctgcc gagttgtctt gtcgataaac ttggtgcgct
    44761 cgactatctg ttcgtcagct caaaaccctt tgccgaaaaa tatttcccta acggcgtaac
    44821 gcgttcggca ttactgaaag cgccagtggt cgcgtttgac catcttgacg atatgcacca
    44881 ggcctttttg cagcaaaact tcgatctgcc tccaggcagc gtgccctgcc atatcgttaa
    44941 ttcttcagaa gcgttcgtac aacttgctcg ccagggcacc acctgctgta tgatcccgca
    45001 cctgcaaatc gagaaagagc tggccagcgg tgaactgatt gacttaacgc ctgggctatt
    45061 tcaacgacgg atgctctact ggcaccgctt tgctcctgaa agccgcatga tgcgtaaagt
    45121 cactgatgcg ttactcgatt atggtcacaa agtccttcgt caggattaat ccatcaaata
    45181 atgcctgata gcacatatca ggcgttgtcc tcacttcttt ttgtattcct tgaatcacat
    45241 cacaaaatag acaaatctca ggcggcaaaa aacgacgtct gaatgcattt tttttgctgg
    45301 cgacaaaccc acgtaaaaag ctcaccgtag gcgcaaatac cctcattttg attgcgtttt
    45361 acggagcaaa taatgtctaa cgtgcaggag tggcaacagc ttgccaacaa ggaattgagc
    45421 cgtcgggaga aaactgtcga ctcgctggtt cagcaaaccg cggaagggat cgccatcaag
    45481 ccgctgtata ccgaagccga tctcgataat ctggaggtga caggtaccct tcctggtttg
    45541 ccgccctacg ttcgtggccc gcgtgccact atgtataccg cccaaccgtg gaccatccgt
    45601 cagtatgctg gtttttcaac agcaaaagag tccaacgctt tttatcgccg taacctggcc
    45661 gccgggcaaa aaggtctttc cgttgcgttt gaccttgcca cccaccgtgg ctacgactcc
    45721 gataacccgc gcgtggcggg cgacgtcggc aaagcgggcg tcgctatcga caccgtggaa
    45781 gatatgaaag tcctgttcga ccagatcccg ctggataaaa tgtcggtttc gatgaccatg
    45841 aatggcgcag tgctaccagt actggcgttt tatatcgtcg ccgcagaaga gcaaggtgtt
    45901 acacctgata aactgaccgg caccattcag aacgatatcc tcaaagagta cctctgccgc
    45961 aacacctata tttacccacc aaaaccgtca atgcgcatta tcgccgacat catcgcctgg
    46021 tgttccggca acatgccgcg atttaatacc atcagtatca gcggttacca catgggggaa
    46081 gcgggtgcca actgcgtgca gcaggtagca tttacgctcg ctgatgggat tgagtacatc
    46141 aaagcagcaa tctctgccgg actgaaaatt gatgacttcg ctcctcgcct gtcgttcttc
    46201 ttcggtatcg gcatggatct gtttatgaac gtcgccatgt tgcgtgcggc acgttattta
    46261 tggagcgaag cggtcagtgg atttggcgca caggatccga aatcactggc gctgcgtacc
    46321 cactgccaga cctcaggctg gagcctgact gaacaggatc cgtataacaa cgttatccgc
    46381 accaccattg aagcactggc tgcgacgctg ggcggtactc agtcactgca taccaacgcc
    46441 tttgacgaag cgcttggttt gcctaccgat ttctcagcac gcattgcccg caacacccag
    46501 atcatcatcc aggaagaatc agaactctgc cgcaccgtcg atccactggc cggatcctat
    46561 tacattgagt cgctgaccga tcaaatcgtc aaacaagcca gagctattat ccaacagatc
    46621 gacgaagccg gtggcatggc gaaagcgatc gaagcaggtc tgccaaaacg aatgatcgaa
    46681 gaggcctcag cgcgcgaaca gtcgctgatc gaccagggca agcgtgtcat cgttggtgtc
    46741 aacaagtaca aactggatca cgaagacgaa accgatgtac ttgagatcga caacgtgatg
    46801 gtgcgtaacg agcaaattgc ttcgctggaa cgcattcgcg ccacccgtga tgatgccgcc
    46861 gtaaccgccg cgttgaacgc cctgactcac gccgcacagc ataacgaaaa cctgctggct
    46921 gccgctgtta atgccgctcg cgttcgcgcc accctgggtg aaatttccga tgcgctggaa
    46981 gtcgctttcg accgttatct ggtgccaagc cagtgtgtta ccggcgtgat tgcgcaaagc
    47041 tatcatcagt ctgagaaatc ggcctccgag ttcgatgcca ttgttgcgca aacggagcag
    47101 ttccttgccg acaatggtcg tcgcccgcgc attctgatcg ctaagatggg ccaggatgga
    47161 cacgatcgcg gcgcgaaagt gatcgccagc gcctattccg atctcggttt cgacgtagat
    47221 ttaagcccga tgttctctac acctgaagag atcgcccgcc tggccgtaga aaacgacgtt
    47281 cacgtagtgg gcgcatcctc actggctgcc ggtcataaaa cgctgatccc ggaactggtc
    47341 gaagcgctga aaaaatgggg acgcgaagat atctgcgtgg tcgcgggtgg cgtcattccg
    47401 ccgcaggatt acgccttcct gcaagagcgc ggcgtggcgg cgatttatgg tccaggtaca
    47461 cctatgctcg acagtgtgcg cgacgtactg aatctgataa gccagcatca tgattaatga
    47521 agccacgctg gcagaaagta ttcgccgctt acgtcagggt gagcgtgcca cactcgccca
    47581 ggccatgacg ctggtggaaa gccgtcaccc gcgtcatcag gcactaagta cgcagctgct
    47641 tgatgccatt atgccgtact gcggtaacac cctgcgactg ggcgttaccg gcacccccgg
    47701 cgcggggaaa agtacctttc ttgaggcctt tggcatgttg ttgattcgag agggattaaa
    47761 ggtcgcggtt attgcggtcg atcccagcag cccggtcact ggcggtagca ttctcgggga
    47821 taaaacccgc atgaatgacc tggcgcgtgc cgaagcggcg tttattcgcc cggtaccatc
    47881 ctccggtcat ctgggcggtg ccagtcagcg agcgcgggaa ttaatgctgt tatgcgaagc
    47941 agcgggttat gacgtagtga ttgtcgaaac ggttggcgtc gggcagtcgg aaacagaagt
    48001 cgcccgcatg gtggactgtt ttatctcgtt gcaaattgcc ggtggcggcg atgatctgca
    48061 gggcattaaa aaagggctga tggaagtggc tgatctgatc gttatcaaca aagacgatgg
    48121 cgataaccat accaatgtcg ccattgcccg gcatatgtac gagagtgccc tgcatattct
    48181 gcgacgtaaa tacgacgaat ggcagccacg ggttctgact tgtagcgcac tggaaaaacg
    48241 tggaatcgat gagatctggc acgccatcat cgacttcaaa accgcgctaa ctgccagtgg
    48301 tcgtttacaa caagtgcggc aacaacaatc ggtggaatgg ctgcgtaagc agaccgaaga
    48361 agaagtactg aatcacctgt tcgcgaatga agatttcgat cgctattacc gccagacgct
    48421 tttagcggtc aaaaacaata cgctctcacc gcgcaccggc ctgcggcagc tcagtgaatt
    48481 tatccagacg caatattttg attaaaggaa tttttatgtc ttatcagtat gttaacgttg
    48541 tcactatcaa caaagtggcg gtcattgagt ttaactatgg ccgcaaactt aatgccttaa
    48601 gtaaagtctt tattgatgat cttatgcagg cattaagcga tctcaaccgg ccggaaattc
    48661 gctgtatcat tttgcgcgcg ccgagtggat ccaaagtctt ctccgcaggc cacgatattc
    48721 acgaactgcc gtctggcggt cgcgatccgc tctcctatga tgatccattg cgtcaaatca
    48781 cccgcatgat ccaaaaattc ccgaaaccga tcatttcgat ggtggaaggt agtgtttggg
    48841 gtggcgcatt tgaaatgatc atgagttccg atctgattat cgccgccagt acctcaacct
    48901 tctcaatgac gcctgtaaac ctcggcgtcc cgtataacct ggtcggcatt cacaacctga
    48961 cccgcgacgc gggcttccac attgtcaaag aactgatttt taccgcttcg ccaatcaccg
    49021 cccagcgcgc gttggctgtc ggcatcctca accatgttgt ggaagtggaa gaactggaag
    49081 atttcacctt acaaatggcg caccacatct ctgagaaagc gccgttagcc attgctgtta
    49141 tcaaagaaga gctgcgtgta ctgggcgaag cgcacaccat gaactccgat gaatttgaac
    49201 gtattcaggg gatgcgacgc gcggtttatg acagcgaaga ttaccaggaa ggaatgaacg
    49261 ctttcctcga aaaacgtaaa cctaatttcg ttggtcatta atccctgcga acgaaggagt
    49321 aaaaatggaa actcagtgga caaggatgac cgccgatgaa gcggcagaaa ttatccagca
    49381 taacgacatg gtggcattta gcggctttac cccggcgggt tcgccgaaag ccctacccac
    49441 cgcgattgcc cgcagagcta acgaacagca tgaggccaaa aagccgtatc aaattcgcct
    49501 gctgacgggg gcgtcaatca gcgccgccgc tgacgatgta ctttctgacg ccgatgctgt
    49561 ttcctggcgt gcgccatatc aaacatcgtc cggtttacgt aaaaagatca atcagggcgc
    49621 ggtgagtttc gttgacctgc atttgagcga agtggcgcaa atggtcaatt acggtttctt
    49681 cggcgacatt gatgttgccg tcattgaagc atcggcactg gcaccggatg gtcgagtctg
    49741 gttaaccagc gggatcggta atgcgccgac ctggctgctg cgagcgaaga aagtgatcat
    49801 tgaacttaat cactatcacg atccgcgcgt tgcagaactg gcagatattg tgattcctgg
    49861 cgcgccaccg cggcgcaata gcgtgtcgat cttccatgca atggatcgcg tcggtacccg
    49921 ctatgtgcaa atcgatccga aaaagattgt cgccgtcgtg gaaaccaact tgcccgacgc
    49981 cggtaatatg ctggataagc aaaatcccat gtgccagcag attgccgata acgtggtcac
    50041 gttcttattg caggaaatgg cgcatgggcg tattccgccg gaatttctgc cgctgcaaag
    50101 tggcgtgggc aatatcaata atgcggtaat ggcgcgtctg ggggaaaacc cggaaattcc
    50161 tccgttcatg atgtattcgg aagtgctaca ggaatcggtg gtgcatttac tggaaaccgg
    50221 caaaatcagc ggggccagcg cctccagcct gacaatctcg gccgattccc tgcgcaagat
    50281 ttacgacaat atggattact ttgccagccg cattgtgttg cgtccgcagg agatttccaa
    50341 taacccggaa atcatccgtc gtctgggcgt catcgctctg aacgtcggcc tggagtttga
    50401 tatttacggg catgccaact caacgcacgt agccggggtc gatctgatga acggcatcgg
    50461 cggcagcggt gattttgaac gcaacgcgta tctgtcgatc tttatggctc cgtcgattgc
    50521 taaagaaggc aagatctcaa ccgtcgtgcc aatgtgcagc catgttgatc acagcgaaca
    50581 cagcgtcaaa gtgatcatca ccgaacaagg gatcgccgat ctgcgcggtc tttccccgct
    50641 tcaacgcgcc cgcactatca ttgataattg tgcacatcct atgtatcggg attatctgca
    50701 tcgctatctg gaaaatgcgc ccggcggaca tattcaccac gatcttagcc acgtcttcga
    50761 cttacaccgt aatttaattg caaccggctc gatgctgggt taatggtttg tcccgtcacg
    50821 atcttcagca atatgctgaa gcatcgtggc ggtataacga tgatttctga ttgaataaag
    50881 atattcctgc atatacatcg gcctgtgcgg cgcatcaaaa tatttcagcg ttgccggatt
    50941 caccagtcgc caggcaaaat gcgggatcac cgtcagaaaa tgtccgcgct ccacggcgct
    51001 gattttggcc ataaaactgt agggacgata aataatggtg ggattgatcc cacaaggtcg
    51061 catattcgca tcaatcattg cttcgaagtt agcccggttt tgaaagcgca tttgtagcca
    51121 gggcagctcc tgaagcagct cttgctcttg acgctcttca taacggcgag aaacgagaaa
    51181 gcccaatcgc agcggtggta attcactgat agtcaggttt tccagttcct gaactcgtgc
    51241 agatacatgc tgtggggaga taataaaatc taactgacgg tcaaaaagat tatcgataac
    51301 accgttttcg ctgaactcaa ctggctgggc ggtcacgcct tcatacttat ctccgagact
    51361 aatcaactga tcgaaaataa ttgtcggata agtattatct atgccaatca caatttctcg
    51421 tgaacgacgc cctgagttat ggatttcatt atcaatcgca gaaagtcgtt gataaaccgg
    51481 gaacagtttt tgatacagtt cctgaccggc tttattgagg ctgatattat tatctttgcg
    51541 ggtaaatagc gtatagccaa tttgttcttc cagcgcggca atgcttttac caaatggcga
    51601 agccgtcata tggatttttt ctgccgcccg ggcaatatta ttggtttgcg ctaataaaat
    51661 gaaattgcgc atctttttcg aaataaaaat gtccatcccc cctccccgca tccttgtccg
    51721 cttaaccgtt tcagtcagcc tatccttgat gaaaccgcga gcaaagatag gtgattacgt
    51781 catggtttta cagaaaatta cagaaaaagg aggcaatatc gggtaaaggc attagcccga
    51841 cgaatacgtc gggctacaag tattattgtg ctgcaggtgt tttagcgggt tgttgatcca
    51901 caggttctaa ctggaagacc acatcgacct gatcatcaaa ctgaatagcg gcctgctcgt
    51961 aagtttcctg ggcagacacc ggcgcggcat cggctttcat catccgcacc attgggctgg
    52021 gctgatagtt ggaaacatgg tagcgcacgc tatataccgg ccccagttta cgatgaaagc
    52081 cgtgcgccag ttcctgcgcc tgatgaatcg cgttatcaat cgctgcctta cgcgctttgt
    52141 ctttataggc atccggctgc gccacgccca gcgacacaga acgaatttcg ttaagacccg
    52201 ccttcagcgc gccatccagc aaggaattca gtttgtctaa ctgacggagc gtgacttcca
    52261 ccgttctcac agcgcggtag cctttcagga tacttttacc atcctgataa tcataatctg
    52321 gctgggtgcg taaattcgct gagctgatat cttttttcgc gatctgattg agttcaagga
    52381 aggaaatgta ttgtgcgacg cgctcatctg cctgtttctt ggctgtagcg gcatccttcg
    52441 cggccacgtt aacttcaatc gcaagagtgg caatgtctgg caccgcatcc acacttgccg
    52501 taccggaggt gacaatatgc ggtccatccg gcaattcgtt agcctgcgct gccatcccgc
    52561 taatacccat taatgccgcc agggcgataa ctttgaactt cacgttagtc tctccgtgtt
    52621 aattgacttg cccaaaattc aggacctctg gaggcaagct tagcgcctct gttttatttt
    52681 tccatcagat agcgcctaac tgaacaaggc ttgtgcatga gcaataccgt ctctcgccag
    52741 ctgcaaggct ataaaccaca tgacacatcc cactaccaga ttgataatgc gctgtgattt
    52801 tgccgtgcgc aggcgcggtg ccagccaggc tgcgagaata gccagaccaa agaaccacag
    52861 gaaagaggcg ctaattgtcc cgagcgcaaa ccaacgtttt ggttcgacgt caagctgccc
    52921 gccaaggctg cccagtacaa caaaagtatc caggtaaaca tgcggattca gccaggtcac
    52981 tgccaacatg gtggcgataa ttttccatct gccttgcttc aggacttcgg cactagctaa
    53041 ctcaatatta ctactcatcg ctgttttaaa agcgccaaaa ccataccaca gcaagaagac
    53101 tacgccgccc caggtgacca gcgccagcaa ccacggcgac tgcatcaata acgcgctacc
    53161 accaaaaatc cccgcgcaaa tcaggaccaa atcgctgata gcgcaaagta aggcaatcat
    53221 aatgtggtac tgacgacgaa tgccctgatt catcacaaaa gcattttgtg ggccgagcgg
    53281 cagaatcata gctgccccaa gagcaagacc ttgaaagtaa taagaaaaca cgttggatat
    53341 tccgaattaa gtatctgatg cagagactat atcgcgggat tgttattagc ggaaattgat
    53401 aatattaatt ggcaatcaga ataactaata agagagaggc cagagagtat tatctggcct
    53461 cagcgttgat tacgcagctt tgtcttcttt cacccgctta aagttcacat ccatttgcgg
    53521 gtacgggaag ctgataccgg cggcatcaaa ttcacgttta atacgctcca gcacatccca
    53581 gtacacgttt tgcagatcgc cgctgttgct ccagacgcgg accacgaaat taatcgacga
    53641 tgcaccaagt tcgttcaggc gcacagtcat ttcgcgatct ttcaaaatgc gatcttcaga
    53701 ctggataata ttggtcagga tctgcttaac ctgatcgata tcggaatcat acgccacgcc
    53761 aataataaat tcgttacggc gaactggctc gcgggagaag ttaataatat ttccggcaat
    53821 aattttaccg ttcggaataa cgataatttt accgtctgca gtacgcatgg tggtggagaa
    53881 aatctgcaca ctcagcacag taccggctac gccgccgagg tcaacatatt ctccggcacg
    53941 gaacgggcgg aacatgacaa gtaacacgcc agcggccagg ttagaaagtg acccctgcaa
    54001 agccagacca acagctaagc ctgcggcacc gagtacagca atgactgacg ctgtttgtac
    54061 acccacgcgt cccagggctg cgattagcgt aaaggcgata ataccgtaac ggactaatgc
    54121 agaaagaaaa tcagcaacag tggcatcgat tttacgggag atcatcaggc gattcaccgc
    54181 gttggaaatc atccgcgcga taatcaaacc aacgatgatg atcgcgagtg ccgccacgat
    54241 gtttactgca taacttagca gcagcgcctg gttagctacc agccagcttc ccgcgccgtt
    54301 tatgctatcg acaacattca aatcttccat tcaatattcc ttttcaactg actcaaaatg
    54361 gagaaataca ctccgccgcc ttatgacggg cagtctgaca agggtaaaca ataaagcata
    54421 attttgccaa atagatcaca gatttctcat ctgaatcacc ttgcttttgg cgaaaaaatc
    54481 cccgcagagc ggggctcaga cagatatcaa tgtgcgcttt gtcatgccgg atgcggcgtg
    54541 aacgccttat ccggcctaca aaaacctgca aattcaatat attgcaggat acgtgtaggc
    54601 ctgataagcg tagcgcatca ggcaattttg cttttggcga aaaaaagacc cgcagagcgg
    54661 gccttgagat aagcagaaag gaatgactta cagaacgtcg atcgcgttca gttcctggaa
    54721 tgctttttcc agacgagcga tcatcgaagt ctgaccggca cgcagccata cgcgcggatc
    54781 gtagtatttc ttgttcggct gatcttcgcc tttcgggtta cccagctgac cctgcagata
    54841 agcttcgttc gctttgtagt agttcagaac gccttcccag gttgcccatt gggtatcggt
    54901 atcgatgttc attttcacta cgccgtagct tacggagtct ttgatttcct gagcagtaga
    54961 accggaacca ccgtggaata cgaagttcag gctgttgtgc ggcaggttgt gtttcttaga
    55021 aacatattcc tgagaatcac gcaggatggt cggagtcaga accacgttac ccggcttgta
    55081 aacaccgtgt acgttaccga aggacgcagc gatggtgaaa cgcgggctga ttttgctcag
    55141 ctcggtgtat gcgtaatcaa cgtcttccgg ctgggtgtac agtgcagaag cgtccatgtg
    55201 gctgttgtcc acgccgtctt cttcaccacc ggtgcaaccc agttcgattt ccagagtcat
    55261 gccgattttg gacatacgct ccaggtattt agagcagatt tcgatgttct cttgcagaga
    55321 ttcttcagac aggtcgatca tgtgagaaga gaacagcggc ttaccggtag ctgcgaagtg
    55381 tttttcaccc gcgtccagca gaccgtcgat ccacggcagc agtttcttcg cgcagtggtc
    55441 agtgtgcagg ataaccggaa caccataatg ttcagccatc tggtgaacgt gatgcgcacc
    55501 agagatcgcg cccaggatag cagcaccctg cggaacgtca gatttcacgc ctttaccagc
    55561 gataaaggaa gcaccaccgt tggagaactg aacgataacc ggcgctttaa ctttagcagc
    55621 agtttccagt acggcgttga tggagtcagt accgacgcag tttactgctg gcagtgcgaa
    55681 gttgttttct tttgctacct ggaaaacttt ctgtacgtca tcaccagtga ttacgccagg
    55741 ttttacgaaa tcaaaaatct tagacatgtc tcttgtcctg tatcgtcggg ccttatactc
    55801 atcatacttc aagttgcatg tgctgcgtct gcgttcgctc accccagtca cttacttatg
    55861 taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagtatg
    55921 aaaaaatgcg tgctctaaaa gcgcgctgaa acaagggcag gtttccctgc cctgtgattt
    55981 tttacttctt agcgcgctct tcgagcatcg ctactgcagg cagtacttta ccttccacga
    56041 attcgaggaa tgcgccgccg ccagtggaga tgtaggagat tttgtcagca atgccgaaca
    56101 ggtcgattgc tgccagagtg tcgccgccgc cagcgatgga gaacgcttcg ctgtctgcga
    56161 tagcgttagc cacgatttca gtacctttgc ggaagttcgg gaattcgaac acgccaaccg
    56221 gaccgttcca cagaatggtt ttcgcattct tcaggatttc agccagttcc tgagcggaag
    56281 catcaccgat atccaggatc tgctcgtcag ctttcacatc gttaacagat ttcagggtag
    56341 ccggtgcagt ttcagagaac tcggttgcta cgcgaacatc agacggaacc gggatgttgc
    56401 aggtggtcag cagacgttta gcttcgtcaa ccaggtcagc ttcgtacagg gatttaccca
    56461 catcgtggcc ttgtgccgcg ataaaggtgt tagcgatacc accaccaaca atcagctggt
    56521 cagcgatttt agacaaggag tccagaacgg tcagtttggt agatacttta gaaccaccaa
    56581 cgatagccac catcgggcga gctggttctt tcagtgcttt acccagcgcg tccagttcag
    56641 ctgccagcag cgggcctgcg cacgcaacgt cagcgaattt accgataccg tgagtagaag
    56701 cctgcgcgcg gtgagcagta ccgaatgcgt ccattacgaa cacgtcacac agtgcagcgt
    56761 attttttgga cagggtttcg tcgtctttct tctcgccttt gttgaagcga acgttttcca
    56821 gaacaaccag ttcaccttca gcaacgtcaa cgccgtcgag gtaatcttta accaggcgaa
    56881 ccgggttaga cagtttgtct ttcaggtagt taacaaccgg cagcagagag aattcttcgt
    56941 tgtactcgcc ttcggtagga cgacccaggt gggaagttac catcactttt gcgccttgtt
    57001 tcagggccag ttcgatggtc ggcagagaag cacggatacg cgcgtcgctg gttactttcc
    57061 cgtcttttac tggtacgttc agatccgcac ggataaatac acgtttccca gcaagatcca
    57121 gatcggtcat cttaattaca gacatggtga atcctctcgt tgattctaaa agttttgcag
    57181 acgctgcttg cgtcttacct gaaagcaaca gtagccatag ctaacgtcgt gtcgagcatt
    57241 cggttagcaa agccccattc gttatcgcac cagaccaacg ttttgatcag gtgtgcgcca
    57301 ctgacccggg tttgggtgcc atcgacaatg gcactgtgcg gatcgtggtt aaaatctaca
    57361 gagaccaacg gcaattccgt atagtcaact ataccatgaa atgcaccttg tgctgctttt
    57421 tgcagcaaca ggttgacttc attggctttt acaggtttct tcaccgtcac gcttaaatcg
    57481 attgccgtca catttatggt tggcacacgt accgcaatcg cttcaaagcg atcgttaaat
    57541 tgcggaaaaa atcgtgtgat accggcggcc agtttagtat cgaccggaat gatcgactgg
    57601 ctggctgccc gggtgcgacg caggtcagga tggtatgcat caataacctg ttgatcgtgc
    57661 atggcggagt gaattgtggt cacagtgccg gactcaatac cgtacgcatc atctaacaat
    57721 ttgatgacgg gaattatgca attcgtggta caggaagcgt tagaaacgat gcggtgttcc
    57781 gcacgaagtt gatcctgatt gacgccgtaa acaacggtcg cgtcgagatc gttactgcca
    57841 ggatgtgaaa agagcacttt tttggccccg gcggcaatat gcgcttcgcc atgctcgcgg
    57901 gagccatata cgccggtgca gtcgaggact acatcaacgc caagttcacg ccaggggagc
    57961 gattgcagtg aacgttcatg caatacgcgg atggcgtcat caccaacaaa aagttgatcg
    58021 cgttcctgtc gtacttccca tgcaaaacgg ccatggctgg tgtcatattt caacaaatgc
    58081 gccatgcccg cagcatccgc cagttcgttg attgccacca cggtaatttc cgcccggcgt
    58141 ccggattcat acaaagcacg aaccacatta cgcccgatgc gaccgaagcc atttatcgct
    58201 acgcgtacgg tcatagatct cctgcaaggt tttccctgag caaatttgcc agacagagta
    58261 atccagcaaa tcgtccgggg aaaccttacc tgtcgcaaac tgcgactgat tggttaattg
    58321 tcgaacattt aatcgactga aacgcttcag ctaggataag cgaaacgtgg aataaaagga
    58381 atgattgtcc agtcaaagaa agcaattatc tgacttacgt cacgttttca gccccgcccc
    58441 tgtggaattt attccacttt gcagaatcac gaataagcgt agcaaaatct tttcgttgtg
    58501 ccgtcacaaa ccctgactta tatcagaata tgactgatgc agcgtcgata ttatccgcaa
    58561 tacgtccgag tttcggagta ctccaatgat gacattgccc tacccatatt cttcctccgc
    58621 acgcttttac gcactgcgcc tcctccccgg gcaggaagtt ttttcccagc tacacgcttt
    58681 tgcacagcag catcaacttc atgccgcctg gattgcaggc tgtaccggta gcctgactga
    58741 cgtcgccttg cgctatgccg ggcaagaagg caccacgctc ctgaacggta cattcgaagt
    58801 gatctcacta aacggcacgc tggaacagag tggtgagcat cttcatctct gcgtttccga
    58861 tccacacggc acgatgcttg gcggtcatat gatgcccggc tgtaccgtgc ggaccacgct
    58921 tgaactggtg atcggtagcc ttgaggaact ggcgtttagc cgccaacctt gcgcgctttc
    58981 tggttacgac gagttgcata tttcccccgt taaataacta ctttcaaaga ggttttctta
    59041 tggcgcgtag ccacttttcc agtcaggcac tggtgttgat cgttatatct atcgccatca
    59101 atatgatcgg cgggcaactg gccagcatgg tcaaattacc tatatttctc gactccatcg
    59161 gcacacttat tagcgcggta ctgcttggtc cggtgatcgg aatgctgacc ggcttactga
    59221 ccaatttact ctggggattg ctgaccaacc cgatcgccgc cgcatttgca cccgtcgcga
    59281 tggtcatcgg cctggtcgcc ggttgcctgg cgcgagcggg ttggtttcgc accctgccaa
    59341 aagtcgttgt cagcggcgtg attattacgc ttgcggtaac ggtcgtcgct gtaccgctgc
    59401 gtactgcgct gttcggcggt gtcaccggta gcggagccga tttgttcgtt gcctggatgc
    59461 actcgatggg gcaaaacctg gtggagtcgg tggcgattac ggtgataggc gcgaacctgg
    59521 tcgataaaat tcttacagcg gtgattgtct ggcttttact gcgccagctg ccgattcgca
    59581 ccacgcgcca ttttccggca atggctgccg tgcgctaaat gcatccattt acctcattaa
    59641 cactgtgggc attggcggcc tgcaccacgc ttatcctgcc cgcgcagacc attttgccga
    59701 tctatagcgc ggcgaccttt ttctgcctta ttgcgctaaa ggccacgcag cggcgggcaa
    59761 aatatgtcgt ctggctgatg ttctcgctgg gagccgggct gtggctggta cacggtggct
    59821 ggctaccaga atggctaagc ggcacgccac gctcgccaga acggtggtcg catgctatta
    59881 cgctctggtt gcggattctg gcgattgttt caacctcaca gttgtggatg cagtacgtcc
    59941 ccgtgcaatg ttttattcgc gctctgtttg cttctcgcct gccgccaggc gtcgcttatc
    60001 tgtttgccgg gccgttattg gtcgttgagc agttaaaacg gcaattagcg attattcatg
    60061 aagcgcagcg tgcccgcggc gtgccgctgg atgagggctg gtatcagcgt ttacgtgcga
    60121 tgcccgcgtt aattattccg ctaacacaca atgcgcttaa cgatctggct gtccgtggtg
    60181 cggcgctgga tatgcgggca tttcggatcc acaaccgacg aacaacgttg tgggcaccag
    60241 ccgatagcac cctacaacgc gtagcgcgtt acaccatgat cttgctgatg ctgacagaat
    60301 tcggagcgtg gatatggtta cgttagaaca gttccgttat tgcccgacac actcaacgca
    60361 tcctccgttc tgttatgact ttcactatgt caaaccgggg atggtcgcca tctttggtga
    60421 caacggcagt ggcaaaagta cgctggcaca gctgatggcg ggctggtatc cggactttct
    60481 gccaggagag ataacaggca cggggacgct cctcggtacg cccattgggc atctcccgct
    60541 taatgaacaa tccgccacca tccagctcgt ccagcaatcg ccctatttgc agctctccgg
    60601 ctgtaccttt agcgtggaag aagaagttgc ctttgggcca gaaaatctgt gtcttgctga
    60661 agcagagatt atggcgcgga ttgatgccgc cctgactctg accgagtgcc agccgttgcg
    60721 ccatcgtcac ccggcaacac tctccggtgg agagacacag cgcgtggtca tcgcctgcgc
    60781 catcgcgatg cagccaaagc tcttaattct tgatgaagcc tttagccgcc tgactccgca
    60841 ggccagcgaa atgctgttgc agcgcttaca acactgggcg tttgaacgtg gttcgctgat
    60901 tattcttttt gaacgtcatc gcacaccttt cctgaactac tgccagcaag cgtggcaatt
    60961 gcaaaacgga gcgctccagc cactatgtta acgttaaata agatatctta tcgctggcct
    61021 ggcgcggcaa cggattgtct ttgcgacatt tctctgcaac tcaaacaagg tgaatggttg
    61081 gcgctgaccg gcgacaatgg cgcgggaaaa tcgacgctgt tacgagtaat ggcaggtctt
    61141 cttacgccca cagccggtac ggttatgctg caacaacagg cgatgaaaaa cctgaaaaac
    61201 cgccagcgcg cggcaaaggt tggcgtactg tttcaggaag cggaaaacca actttttcac
    61261 agcaccgtcg ctgacgagat cgcatttggt ttgaaactgc aaaaatgccc ggcggatgaa
    61321 atcactcaac gtacccatgc tgccctgcaa tgttgtcagt tagcagacac tgccaactcg
    61381 cacccgttgg atctgcatag tgcgcagcga cggatggtcg ccgtcgcctg cctggaagca
    61441 ctctctccgc cactattgct gctggacgag ccgagtcggg attttgacga aaattggttg
    61501 agcgtgtttg aaagctggct ggagaagtgc ggccagcgag gcacgagcgt cgtagcaatc
    61561 agtcatgacg ccgcatttac ccggcgtcat ttctctcgtg tggtgcgact ggaagatggt
    61621 ctaatcagga acgttaatcc accagacgat attcacccgt agcggtcatc attaaggtca
    61681 aatttgcagg cagactctct tccagccccc ggcgaacatt cggcccgtcg gtacggtcat
    61741 aaaaagcttc tgcgtctgcc agcgataccc ctcccgccag cttgcgccct accagtcgtt
    61801 cccgcaacgc agtagctggc gcgttgataa agatcgagaa atcacaaaat tgcgcaagct
    61861 ggcaccactt ctcgtcgtcc agcaacaacc agtttccttc gacgatgacg agtggtgcgg
    61921 taacgtgcaa cgcatcttca acaggatcat gcttttgtcg atcatactgc ggccacgtac
    61981 aatccccttc cactacctgg cacagatttt ccgccagttt cgcaacgtca aatgtctctg
    62041 gtgcgccttt gaaggggcgc aattgatgcg catccagcca gctattgtaa tggtgaaaac
    62101 catccatcgg gagcgtctgg atagcgggta attcagggtc ttgttgcgcg agatattccc
    62161 agaaggtagt taaggtagat ttacccgtcc ccggcggagc gcaaagaaaa accaccgtac
    62221 gtcgctgcgg acttaccgtc tgtaaggccg ccagcatacg cagcaacggt ttatgcacgc
    62281 gttctatctc ttcatcatgg tactgcgctt gcacgttcag cccattcacc gttaattcaa
    62341 ttttcacggc gttaattcct ttaaggtggt cgttactgcc agcgtcagcg ccgttttgac
    62401 cattccgcta aaacgcgcct ctgaccagac ggcgaaatcg gcgaacttca tcgcttttaa
    62461 cgcgtcatag agcgtcgcat tgcaggttat gctattgacg ttgctgataa aatcccaggt
    62521 catgtcatcg gcaaatccgg gcgtttcatc ctgttcgttc aggtaatgcc agtactggga
    62581 aaagtgcgtg atatcggcat acagccattt atccattttc cccagcgcat aaatcaaacg
    62641 cagcgcaaca tcaatatcgt caagcgggcc gctttgggcg agtaacggct tcactgcata
    62701 ttcgacgatc tcttcatcgt tatcgacaaa cagcgacggc agaaactggc gtatcccctg
    62761 gagcaaaata gaatgcgcag ttttcataaa tgaaaataaa ttgtcctgtg catccagttg
    62821 ctcaagcaca tcatcttctg tcagcgtcgc cattggtctt ccgatagatc accatcaatg
    62881 cagggagtct attatattac acgtttggtc cgcgccgccg atcagtaatg tctgcgtccg
    62941 caccccattc gccgtctgtt ggataccgtt caccaactcg ccgcccgtca cgcccgtagc
    63001 gctaaagagg atatcgttcc ccctcaccag ttcatcgagc gagtacacac ggttaacgtc
    63061 aacgcccatt gccttacaac gcttgcgctc ctgctctgca atctgccgat tttccgtgta
    63121 atccccttta gcctgacaaa agtcaatcag ctcggcctgc atatccccac ctaatgcttt
    63181 aacggcacag gcagaaatca cgccttctgg cgcaccgccg atggtgtaca tcacgtcgta
    63241 aggattatcc tgccagcagg tcagcacgct ggcggcgaca tcgccatccg gcagagcgaa
    63301 aaccttcacg cccagctgtg ttgcttcttc aatcgcggca ctcaggcgcg gtttatccag
    63361 cgtaaccatg cgcagcttgt ccagcggttt acctaacgcc cgcgccacgt tacgcaagtt
    63421 gtccgccaga ggcaaagaca gatcaatcgc cccggcagcc agtcgattaa caaccagctt
    63481 tttcatatac atatcgggcg catgtaacag gctatctcga ggggcgaaag ccatgaccgc
    63541 cagcgcattg ctctgcccca tcgctaccat ccgcgtgcct tcaatcgggt caaccgcgat
    63601 atcgacttct ggcccatcgc ctttgcccac ctcttcgcca atccacagca tcggcgcatg
    63661 gtcaatttct ccttcgccga taaccactcg tccacgaaaa gcgacgtcgt ttaatgcctg
    63721 gcgcatcgcg gtgaccgcca ggccatcaat tttatttttg tcgccacatc cggtttgcgg
    63781 ccaggcagcc aacgctgcct gttccgtaac gcgaaataat ggccacgcca gggacatcat
    63841 tgcgcggcct ccccgatatc aacgccaaat tgtgccagca gatatttctc ggcctgttca
    63901 ttccagatgc cgtgcgtttc ctcaaccagc cgcgccaact ctttaaacag cggatcggtt
    63961 ttgccttttt cggcaaaatc ggcaatggca gtgagcggca tggtcacgcc gttataaatg
    64021 agttttttac cgccagggat atccggcaga ttgagcacgg tatctggcac cgcatccagc
    64081 ccaccgatat gcgtgaccat aaaggacggc tgcaactgcc ccgtggcgct taacgcaatc
    64141 gcctctttca tgtcgtccgt tgaaccgccg gatgtaccga ctacgtgcgt gctattgtaa
    64201 tggacgttgt agaaattaaa cggcactttg aagtttttat ccgtcggccc ggcaaagaag
    64261 ttcagacagc catcttccgc cagtaattcg tcagccatct caacgacagc aggcaccgcc
    64321 gcataaacaa aaatgtcatc gaacccgaca tctcctgtca gcgcccgcag cgtctggacc
    64381 ggatcgctca tccctttggt attcacatac accagctcaa tgcctttact ggccgccaga
    64441 tccaccggca gcagcttctg cacctgcgcc aggcgtttgt cgtcgatatc gaccaccacc
    64501 acccgtgacg gttgtatgcc accgttaatg gcgtaatcga tagcgccaat gcccatcgga
    64561 cccgcacacg ccagcagtgc aatattgccg ccaggtttga cgcccatgcg atgctcataa
    64621 acatattgcg tggtgtgata attggcatga taagcaccaa taatgcagca cataggctct
    64681 gccagcgacg ccgcagcaaa ataagagccg tgatacggta atacgcagcc caaattaatg
    64741 gcgatttcgg gaataatcat ataggtggcg ttaccgccaa aatattcgta gctgtagcct
    64801 gctgaatatc cgcttggtaa ccccatcgcc ggttgcaata caaaacgctg gcctttttta
    64861 tatttgccag tgagattttt acccacttcg acaataaccc cggcacattc atgcccggta
    64921 atgaccggat gattttctaa atcgtcgggt acgcgtttat gttcactacc gagtaacgcc
    64981 gctttccagg tcgataaaca gacgctgtca gaaattacac tcactaacag ttcattatcg
    65041 gtaatttctg gcagttcaaa ttcgcgcaga cggacatccc gcttgccata aatagcagca
    65101 actttggttt tcatttttac ctctgtaatc agtgtttatg ttcggcggtt aattgattaa
    65161 ataaagtgtc gagttttgga tcgccaatat agttattaat caatatcagc ggtttatccg
    65221 tcacacgttt cacgcgccct tccagactgg cgtgagtgac gacgatatcg gcatccgcag
    65281 gcacgttttc tatcgcgtaa tgttttactt ctattgccag ccccgctttt tccaggcgtt
    65341 tacggaaggt ggtcgcgccc atcgcactgg agcccatccc cgcatcgcaa acaaaggcga
    65401 tacgcttaac cctgcttaac gagaatgcac cctcttgttt catcgcctta accgcattgg
    65461 ctgactgagc aaactcatct tcgctctccg tttccaccgt tttttccatc ttcagtatca
    65521 gcgaagtaat agcaaaggac accagggtac ctaccgtaac accggcaatt gtcgccagga
    65581 acgagccttt cggcgtcagt gccaggtaag caaagataga ccccggactt gggccagcca
    65641 ccagaccacc gtccagtaag ttaaacatcc aggtaccaga cataccgccc gcaatcatgg
    65701 caataatagt cagcggcttc atcagcacat atgggaaata cagttcgtgg atcccaccga
    65761 ggaagtgaat aatcatcgcc ccgggcgcag aacgtttact catcccttta ccaaacaagg
    65821 taaacgccag cagcagcccc aggcccggac ctgggttaga ggccaccata aagaagatgg
    65881 atttaccgtt aaccgaagcc tgttgcattc ccagcggata atagacgccc tgatcgatcg
    65941 cattattaag gaaaagcact ttcgccggtt cattgatgac tgacagcaga ggcagataac
    66001 ccgcatgtac cagcgcctcg atacactctt tgacgaaagt attggcaatt aacaccgccg
    66061 ggccgatcac ttcaaaaccc agcagacaaa ggagcatccc cgcgatacct aatgagaagt
    66121 tattgataac catctcaaaa ccggcaggta tgcgtttttc cagtgcctta tcgacatatt
    66181 ttatgaccaa cccaccgagc ggccccatga tcattgagcc aaggaacatc gggatctctg
    66241 cgccaacgat cacaccaata gtacctattc cgcccatgac cgccccgcgt ttaccgccga
    66301 ccagatgacc acctgtagaa ccaatcatca cgggcaataa ataggtaatc atcgggccga
    66361 caattttggc gaaatgttcg ttaggcagcc aaccggtggg aataaataac gcagtaataa
    66421 aaccccaggc aataaaagca ccaatattgg ggatgaccat tgcagtcaaa aagcccccaa
    66481 aagcctggac ctttgcacga gcagacttgt tttccataat attatcctgt agaggagaga
    66541 gtaattatcc gcggctgata atgtccgcta attgtttttc tgatgatgct gttaagagtt
    66601 gttcgagtat ttcttcttcg cacagtaatt cacttaacgc ctgaatagcg ccaatgtgcg
    66661 aatcggcatc ggcagcagag agtccgatga gtaatttaat tggttcgtca ttccccggaa
    66721 aataaacacc ttgttcaagt aatgtcaaag acatcccggt tttaagcgcc ccacattccg
    66781 gtcgcgcatg aggcattgcc acgcctggtg cgagaatata ataagggcca ttgttaatgg
    66841 tggaatcttt aattgcctga atgtaattct cgctgatata gtttttatcc agcaatgata
    66901 ccatcgagaa atcgatagct tcctgccaat cttttgctga atgtataact gagattgatg
    66961 attctggaaa ataatcacta agccgcataa attatcctta ttttattttt agggtaccgt
    67021 ccggcggtaa aaataaaata ctcgtgcggt ttaatgaaag caattgagca gggaaaccga
    67081 tgttttgaaa acagtgaatt attaatttct tatataacat tgagttatag atataacaac
    67141 aaatgtcaat acgcatatcg tgatgcatat tacataattg atatttattg catataaata
    67201 tttgtgatct acaacacgct ttatctattg cttgtccgca aacggacata tcaaggtaat
    67261 aaaaaaggtc gccgaagcga cctttttacc cgaaatgcta attacagcag ttcttttgct
    67321 ttcgcaacaa cgttatcaac agtgaagccg aactcttcaa acagcagctc tgccggagca
    67381 gattcaccga aggtggtcat accgacgata gcaccgttca ggccaacata cttgtaccag
    67441 tagtcagcaa tacccgcttc tacagcaacg cgtgcagtaa ccgctttcgg cagtacggat
    67501 tcacggtaag cagcatcctg cttgtcaaat gcgtcggtag acggcatgga caccacgcgc
    67561 gctttcacgc cttcggcagt cagtttttcg taggcagcaa cagccagttc aacttctgaa
    67621 ccggtagcga tgaaaatcag ttccggctga ccggcgcagt ctttcagcac ataaccaccg
    67681 cgcgcgatgt ttgccagttg ctcttcagtt cgttcctgct gcgccaggtt ctgacgggag
    67741 aggatcagtg cggtcgggcc gtcctgacgc tcaacaccgt atttccacgc gaccgcggat
    67801 tcaacctggt cacacggacg ccatgtagac atgttcgggg ttacgcgcag agaagcgacc
    67861 tgctctaccg gctggtgagt cgggccatct tcgcccagac cgatggagtc gtgggtgtaa
    67921 accatcacct gacgctgttt catcagcgca gccatacgta cggcgttacg tgcgtattcc
    67981 acgaacatca ggaaggtgga ggtgtacggc aggaaaccac cgtgcaggga gataccgtta
    68041 gcaatcgcgg tcataccgaa ctcgcgaaca ccgtagtgga tgtagttacc cgcagcatct
    68101 tcgttgattg ctttagaacc agaccacagg gtcaggttag acggtgccag gtcagcagaa
    68161 ccgccgagga attccggcaa cagcggaccg aaagcttcga tagcattctg agacgcttta
    68221 cggctggcga ttttcgccgg gttagcctgc agtttagcaa tgaactcttt tgctttcgcg
    68281 tcgaagtcag acggcatttc gcctttcata cggcgggtaa attcagcggc ttcctgcgga
    68341 taagctttcg cgtaagcagc gaatttctcg ttccatgcgg attctttcgc ctggcctgct
    68401 tctttcgcat cccactgagc atagatttca gacgggattt cgaacggtgc atatttccag
    68461 cccagctgtt cgcgggtcag ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg
    68521 tgagtaccgg ctttgttcgg ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac
    68581 ggtttgtcag tcactgcgcg cgcttcttct actgcgcgtt tgatggatgc cgcgtcatga
    68641 ccgtcgatgt cgcgaataac gtgccagccg taagcttcga aacgcattgc ggtgtcgtcg
    68701 gtgaaccagc cttcaacgtg accatcaata gagataccgt tgtcatcgta gaacgcaatc
    68761 agtttaccca gcttcagcgt acccgccaga gagcaaactt cgtgggagat gccttccatc
    68821 atgcagccgt cgcccatgaa ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg
    68881 ttaaactgcg ccgccagcgt tttttctgca atcgccatac ctactgcgtt ggcaataccc
    68941 tgccccagcg gaccggtggt ggtttccaca ccagcggtgt aacccacttc cgggtggccc
    69001 ggagttttag agtgcagctg acggaagttt ttcagttctt ccatcggcag atcgtaaccg
    69061 gtgaggtgca gcaggctgta gatcagcatg gagccgtggc cgttggacag cacgaagcgg
    69121 tcacggtcag cccaggacgg attctgcggg ttgtgtttca ggaaatcacg ccacaggact
    69181 tcggcaatgt cagccatacc cataggggca cccgggtgac cggatttggc tttctgtact
    69241 gcgtccatgc tcagcgcacg aatagcattg gcaagctctt tacgtgagga cattttgact
    69301 ccagatcgga tgatgaaggg cacgccctta acgacttgac gacagcgcgt tttgggctac
    69361 gccggaaaat ttgccaacaa tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt
    69421 tgccgcttca gaaatctctg gatcatgctc gcatgttgcg caatctactc gcccgtccgc
    69481 tgcgcttttc cttatactga gactgagcgt cgattcacct gcaaacggcg catttttaga
    69541 ataatcctga ccttgtgcgg aagagaaaac atgaaaattc gcgccttatt ggtagcaatg
    69601 agcgtggcaa cggtactgac tggttgccag aatatggact ccaacggact gctctcatca
    69661 ggagcggaag cttttcaggc ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat
    69721 caggcatgtc aggagatgga cagcaaggcg acgattgcgc cagccaatag cgaatacgct
    69781 aaacgtctga caactattgc caatgcgcta ggcaacaata tcaacggtca gccggtgaat
    69841 tacaaagtgt atatggcgaa ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc
    69901 gtctatagcg ggctgatgga tatgatgacg gataacgaag tcgaagcggt gatcggtcac
    69961 gaaatggggc acgtggcgtt aggccatgtg aaaaaaggaa tgcaggtggc acttggtaca
    70021 aatgccgtgc gagtagctgc ggcctctgcg ggcgggattg tcggaagttt atctcaatca
    70081 caacttggtg atctgggcga gaaattagtc aattcgcaat tctcccagcg ccaggaagca
    70141 gaagccgatg attattctta cgatcttctg cgccaacgcg gcatcagccc ggcaggtctt
    70201 gccaccagct ttgaaaaact ggcaaaactg gaagaaggtc gccaaagctc aatgtttgac
    70261 gaccatcctg catccgccga acgcgcccag catattcgcg atcgtatgag cgcggatggg
    70321 gttaagtaag tcagacatac cgatctacaa cataattgta ggtcggtaac gcctgaatcc
    70381 ccgatcagag tagtgatttc actttattta gcaaaacggc tacatcatcc ggtcgggtat
    70441 atcccgtatc agggtctata atcgagctgt aaacatgtgg cataatgcgc ggcacccccg
    70501 cctccagaca agttttcaga atcacactga agttatccaa atcaatcccg ccagtcggct
    70561 caatcagcgt cataccattg cgcgccgccg tggtcgccag cgcgtatagc tccggaagcg
    70621 acctctcgcc gcccatcgga aagaacttcg ccgcgtgtgc gcccatatct agcatcattc
    70681 gcaccgccgt atcgcaggag acgcgggcag gcgtaccctg gctgctgcta acgccggtgg
    70741 aaatcagcac ttcgcccggc gtccccgtcg ggctaaccag cgcgttgaca tgggtctgct
    70801 caccgcccgt agccgcaagc gcgcctgccg cgaaaccaca accggtaaag gtctggttaa
    70861 cgtgtgctgg atgaatctgc gccgcaatca tcgcagcttt gtaatactgc gccgggtcac
    70921 ccgcccccag acctactgaa atggacggta tttccgccat ccaacgttta acttcctgga
    70981 tcccctcatc caccgaggag aactgcgcgg aaagcacgcc aatcactgcg tggccttcgg
    71041 cggcgtcaaa gatttcacgg gcattggcga tatctttcgc cagcacatta attgccacgc
    71101 gattacgata gaaattaatc ttttgcatga tctgcaatct ccttcagtcg cgccacgata
    71161 agtgccattt ccccttccgc caccgtacgc ggatcaaggc taaaaacgcc ctggtggagg
    71221 ttataccgcc gtgcataaat cgcgatatcg ccaccacgca gttgcgcttc gacgacgcga
    71281 gcgtcaactc ccaattcctg cgcatttact cgaatacgaa tacgccagat tgcacgcccg
    71341 gcttcatcct gctcaatatc agccgttaac ccgtgaatcg cagaaatcgc ttcaaccact
    71401 ggctggagct gttccgcggt gatgactgcc tgcccctggt gataattctc cagcgcgtaa
    71461 accagcccaa ccatgttctc cttgccgatt ttcatcgccc tggcaatgcc gtgatgctgc
    71521 gctttgcagg ctgcaatcca gattttttta ccagtgataa ccccggaggt cggcgcgtta
    71581 aaggcttttg cgccgctgta gaccaccata tccgctccgc tcaccaccca ggcacgtaaa
    71641 tcctcttcgg cggcggcatc gacaatcagc ggcaggttgt acatctgcgc gacatgtgcg
    71701 aagtcctcga tgttcaacat tcccttctgc acgcagtgat gcgacttcac atacagcagc
    71761 gcggcggtcg cgtcggtaat ggcactctcc agctgccaga gagccgccag gttacttgaa
    71821 cccacctcca caacgcgccc gccacctaag cggatcgcgc tgaggatcga tgcaccgtaa
    71881 tcgacgttat ggccgcgcag catcaccact tcattggcca tgccggagct atccggcatg
    71941 agcgccaccc gggccttgtc gccgcgtgta atggcggcgg cgacagcgat ggcgatccct
    72001 gctgaggcac aggaggtaac gtagctatcc tccgccccgg taaagcgaga gaccagctcg
    72061 ccggttttgt ccaccagctg gtcaatctcc acgaaagagg atgccgcgcg cgcggtggcc
    72121 tgcatcacct ccggcgcgac gctggagacg ccgagaatcg tcatcttgcc gcaggcgtta
    72181 atcacctgtt tcagtcccag ttgttgatag atattctgcg tcatggctta cagcactccc
    72241 agcagcgagc agaccacgct caacgccaca atcgacagca gaatcgtggt gtaacgcgga
    72301 ccctttttca ccaaatagaa gtagatggcg aacactgccg ccagcggcag caggccgggc
    72361 gcgatggagt ccagaatctg ctgtaccacc acttcagagc ctttcagtgc gctaattttt
    72421 aaaggtgagg ttatcttgac aaagctcgcg gatagcgcgc ccatcatgat aagccccagt
    72481 acgttagcgc cgtagataag ctccttgatg cgccctccct gcagcagact gataatcgaa
    72541 tcgcgcccca gcgtatagcc cttgtgcacc atgccgtagc tgattgccag cgtaatcgcc
    72601 gggtagagaa tcagcggcaa aataccaccc atcgcgctgc cattggcggc gaacgggata
    72661 aaaatagcaa tcaacagcgg catcaccgcc gcccagatga tggagtcgcc catcccggcc
    72721 agcggcccca tcaggccggt tttaatcccg gtgatagagg catcgttaat cgcctcgccg
    72781 cgggtctttt gctcttccat ggcgatggag atcccctgga tcaccgcgcc aaacgtctgc
    72841 tctgagttga agaagttcag atggcgtttt aacgcctcca cctgctcctc cttttgcgga
    72901 tagagctttt tgataatcgg cgtcatcgag gcgcagaaaa tcaggctctg taaacgctca
    72961 taggagctgg aaacttccgc gcccagccag tagataaacc acgctttggt gatatccgct
    73021 ttggtcagcg ccccggactc ccgcgcgcgc tcaaccagtt catgctgcat tacgtcgtcg
    73081 ctcattatgc cgctccttca tttttcgcca ggcctttaat caggaaggcc acgcaggtgc
    73141 cgaaaatcgc catcgccatg atgtccacct tgaggtacag caccgcgaag aatccgccga
    73201 taaaccacgg cagcaggctc tttttaccaa tcaccataat ggtgatggcg aagcccagcg
    73261 ccgggagaat cccgcccatg atttcaaacg agtgggtcag ccagtgcggc atcagcttga
    73321 ggaaactttc cactacgttc tggccgaagt agttggcggc gaacaccact gggaaacgta
    73381 gtaccagccc cagcagcgcc gggtagagaa aggcgcagcg cataatggct gacatattgg
    73441 ccgtttccgc gtgtttatcg gccatatgca cccaggccgc atttagcgtg cgacgcaact
    73501 ggtcgaggaa tacgccgata acgccaaatg gaatcgccag tgcaatcgcc aggttcgggt
    73561 ccatattggc cttaacggcg ataggaatgg cgatacaggc cgccagcgcc gggtctgacg
    73621 gcacattgcc gcctggcgtt gaggtcacac ccagatagac caactgcatc cccgcgccga
    73681 taatcatcgc cgcaggcata ttgccgagca gcaggccgac aaagaccgcc accaccactg
    73741 gctgtaacag catggcggag aaggtatagc caaggcgcaa ccgggcaaac cagtaataca
    73801 gccccatcag gcttgcaaaa agtaaggtat ccataatttg ttatcccatg ttcagagggt
    73861 cagaattttt tcaggatatc gtccagcgac tgcggtttat cttccgggat ggtctggaaa
    73921 acagccttaa tgccgcgact tttcaaatcg ttaagaatgc caacgtcttt ttcatccagc
    73981 gttatgttct ggaacaccgc tttacggttt ggcccgccgc ctaaaccgcc aacctgaata
    74041 tcagccacgt caaagccctg ctgcaccgct tcctgaatcg ccgccagcga cgggaacagc
    74101 accagcacgt tgccgtcgcc cagctggttc tctttccacg cggccgcgaa gctctggttg
    74161 ctaaagcagt ccactttgat attcggcggc gcggccatca ggtagatatt tttcataaac
    74221 ggatcggcgt ccagctcgtc gctgaccacc gcaatacggt tggcctgaga ctgccctacc
    74281 cacttcgtca ccacctgtcc gtggataaga cggctgtcaa tgcgacataa cacgatgttt
    74341 gccatacgat tttccttact gttgtaattg acgaatatgg gagacgacgt ccaggcaact
    74401 gttgcgccca gcttccacca gatcggccac gcaggcggtc agtgggccat gttcgcgctt
    74461 gtccagcgcc tccagcagca gcgaggcatt aagcccggag atcaccgcca ccggataatc
    74521 ggcgctcagc cgcgccgcca cgttagaggt ggtgccgccg ataaaatcgg tcacgataag
    74581 cgaaccttcc ggcatggttt tcaccacggc ttcgacgcgc tggtaaaact cgcccagcgt
    74641 atcgacgggc atcagcgcga tttccgtcac gccttcgatt tcgcccatca ccatgcgcag
    74701 actgttgcag agctgcagcc cccagccgcc gtgagttagc agcagaattt caggtaacgg
    74761 atgaataggt acagttgtgg tcaaaatagc ctcctttgct ccagtgttta catttcatca
    74821 ggagcaaagg acgtgccaga atttgtgtca cgcacagagg aaatgagatt ggatggggcg
    74881 ttaagccccg taaaccaggg gatcagctgt agagcagttc gtagatataa aaatattctg
    74941 cgtccgataa acggatagcg taagcctctt caatcggcaa aaaggaggat ttgatgacgc
    75001 taaaggcacg agcgtccaga tcaagccggt tttctagcgc catctgtaac ggtttgcggt
    75061 taatcacgat acgctcgacc atgcagcagc agtggatcag aaaacgtagt gtcacctggc
    75121 ggctcggttt cagggagagc gtgttggtta agtagttcag aacatcctcg atctctttca
    75181 gaattcgctg cgggttgagc accgaaatat ggttgatgat gctctccatg gtcagcgcgc
    75241 tgataaagcg catggcgctg cgctccatct ccagtcggcg ctcgctgctt gagagatccg
    75301 gcgtcagcag gctgagcacc agctccggcc cttgctcgga aaacagttcc tccagcgaga
    75361 taaacggtat atccggcagt cccggctgga aggtgccgac gatccctgcc agccgttcac
    75421 tggcattaag cgcctgctga atacgctcca ggctgcgcac ctcgttatag tcgagaatca
    75481 ccatacgcgt gtcctgcgac atcagctcgc caaagctctc ctccagcacc tttttgatct
    75541 tttccgccgt ccccattccg gttatacatg agataaccag cactttgcca ccgctctctt
    75601 gctgcggcgt gcagagccga caagggatat ttttactctg cataagcaca gccaattgcg
    75661 atagatcgcc agtttcgtag cttaaatcca gcccaacctc cagcaggctg gtcagagtga
    75721 tatttggcaa caacagaacg tcaatctgga acagcttgct gatggtgctg ccaaaatgga
    75781 ccagcgatcc gatatccacc atcagtatca gccgctgcca gcaccggtcc tgaatcatcc
    75841 gggtcagggt ttccagggtg tcatgcaccg actgctcaaa tggcatatca attgcgtaga
    75901 acagatcgcg ctccagcacc cggttcacat attgcgcctg gctcgttgcg gtggtcgcac
    75961 cgtgagcgat tagaatcacg ccgcaatccg ggctggtgtc gatgcgctgg cggtagtggc
    76021 gacactcttt caggaacagg cacagccaga ccacctccgt cgccgggcac tgaatatgca
    76081 acaactcatt gattttgcgg cacagaagcg tggcattgtc atactcatct ttacagcgat
    76141 ccagaatcag gctggatgaa tagagttgag gtataagccc tcgccgcaca tagccaatca
    76201 gcgcaagaaa gtgtttacgt aacgggttcg ccaggttttc tggcaatgta aatcctaata
    76261 cctgctccac gtaaccgatc agcagcgtca cacgctcctc aatttgatcg ccatagcgcg
    76321 gtggatgcgc cgcactatgg cggctataaa gaccatattc aaaaatcgaa ctgagtttgt
    76381 ttttcaggat agccagcgtt tctgcgggcg gcacgttact gttgcgcaga ttgacatatt
    76441 cacgagtcag gaaactgtaa aacaggtcgc tctcttctat ttccgcgctg gtcgccaacg
    76501 aattttttaa cgcgggcaac gtacgcgcat ccacgttcag acgatcttta ccgccaaaca
    76561 acgcatcgac cagcagacgc tgctctggcg tggtattaaa cgacatctcc gccagtcgtt
    76621 tatctaactc tagcgtatca ttacgctcgg tcattcccga cgcccacgcc tgcgcgcaca
    76681 agaactgaat atcactttta agctggccga tattgccttc caggggttta cttaacaacc
    76741 aatgcaagag cgttttatca aggcttatgg tacgttcaat tttacgactt tcacgctgaa
    76801 agaagcttac aatcaattcg agctgctcct cgaccgaacg ctggcgaata cccggtaagt
    76861 caatacatac ctggatacgc cgctggaagg tacgcaatag tgccgagttg accgactctg
    76921 tagtggcgca aataaggcgc accgaaattg aatatgttgg cccacttgaa ccaagtgggc
    76981 ggtattcacc tttatcgaga atagaaaaga gcttctcttg tccttcgtac gggaggcgat
    77041 gcacttcatc cagaagtaaa taacctccgt cagcttgctc caccaatccc gttttattct
    77101 cacttgcccc ggtaaatgcc cccttatggt gaccaaacag atgtgaggaa agcaactccg
    77161 gattatgagc atattcggca cagttaaagt aaaccaacgg aggtggcgaa cctatggtgt
    77221 gcttgcaagc gaagcaatgc attaactcag caaagaatgt tttgccaaca ccagatgcac
    77281 cggttaacag cacatgtagt ccgtggggat aaagcaccgc tgcgcggcct ttttctaccg
    77341 catcacggag actacggtcg tatccgataa gtccggaaaa cggatcatca tctgtatatt
    77401 tttctttctt tggcaataaa ctagccactg aatgaactat gcattcgcaa tcatccagtt
    77461 ttcgattaat gagcaattct agtgcgtgac gatgtagaaa aaaaacagga cgtccctgac
    77521 tttttaccac caggccttca ttccataatt gattaagatc tttactaacc gagttgcgtg
    77581 ccagcccaag gttaaaacca atcgcctcag cagtaaatgc tttctccttc gctaaatcag
    77641 acagttccag cccatacgtc agccgctcca gctccttgtg aataacatca atccgtctca
    77701 tttctttacc gcttaaaaaa caatagttgt tcagaacata cactatttgt aaacgtaaga
    77761 atgagaacta tcgggaagaa ttaatgttcc ttgtggcaat ctggtttctg aatattcgtg
    77821 ttttaaatat tacttatggt ctgaaacaac caggcaggtg aacagagcta agaaaaagta
    77881 gcgtagaaat ataaaaacat atatcgcact gatgaatgtt tcccggcatg actatgcaca
    77941 ccgggaaata tttagtaaat tactcgccct ttttcgccgc ctgaatatac agcatctcca
    78001 gcgccagcgt cgccgcagcc agagcagtga tttccgactg atcgtatgcc ggagccactt
    78061 ccactacgtc catcccaacg atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc
    78121 gatcggaggt cagaccgcca attaccggcg taccggttcc cggcgcaaat gcaggatcca
    78181 ggcagtcgat atcaaaggtc aggtaaaccg gcatatcacc cacaatttgt ttcacctggg
    78241 cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg gtaaagccgt
    78301 tgtctttatc aaattcggta cgaataccaa tctgcacgga atgattcgga tcgatcagac
    78361 cttctttcgg cgcggtatag aacatggtgc cgtggtcaaa ttcacaaccg ttcgcgtagg
    78421 tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac
    78481 gcagcagcgg cagcgtaaca aagtggtcac caccgaagga gagcatacgc ttaccggcag
    78541 ccagcagctt ctcggcgtgc gcctgcagct tctcgctcat ctcacgggca tcgccgaaag
    78601 catataccag atcaccgcag tccacgacgt tcagacgctc acgcatgtcg aaattccacg
    78661 ggaagcggtt gtgttcccag gccagattcg tcgaaacctg acggatagct gccggaccgt
    78721 ggcgtccccc cgcacgacca gaagtggcca tatcgaacgg cacaccagta atcacccagt
    78781 cagcatcgct gtcatacggc tggaagttca tcggcaggcg taaaaaacca aaggcattgg
    78841 aaaccagtga gttatcgtat tgatgaccta aggtgctcat ggatggactc ctattacaaa
    78901 gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga
    78961 aacacaagtt tgtgctaatt atcgccttaa atgggcgagg catcaagcga ggttgctatt
    79021 tccgacacgg tggcggcgca tagccattgt tatctgccgg aaaggcttgt ccagagcacg
    79081 gcggcagcgc gttttgcgcc tggccttgta attcaaaggc ggcaaagcct attacaccgg
    79141 tattacgcac atcgccttgc gccgaattgg ctgcgtagga atcagcaacg ttggcgaact
    79201 gaaaagccgc ttcggtatgt ttatctttgc ggaatccttt aatcgctaaa gaatctccgg
    79261 cattcacaat atagccgttg ttattcagcg aaccttgctt gccgttcagc acatccaggc
    79321 catcaacagt agcaacgatc tcgtagttgg tgttacgact gtaattacgg acatataatt
    79381 gataattcat tccgactctg gcatggacaa tatagcgtgg ttcattattg gatgaatcat
    79441 aaacacgcgt aataggtaga gaattaaaat taccatcgcg aatagcatat tccagttcgt
    79501 taatacgaat gctataaacg cggtcatagc cggaaggaac attagtcgaa taactaataa
    79561 cgatcatctc tgccggatcg cgccaggctc gctcagcaga tacgccctga accgatgagt
    79621 gcacatcccc accccaggca gtgccgacat tgcccgatgc actgctatta tactgcgcat
    79681 tactggaaca accggccagc agcacgatta atgccaccaa cgcgctacgt actttccatt
    79741 ttttcataac cgttccctct gcatttaatt aatgcagcga ttgtagggag attatttaat
    79801 aagaaaagca gcgagattat ttttaagaat atataaaaag aaagcgggcc gatttacgta
    79861 aataaatcga cccgccagcg gaataatctt aattattcat cttcaagata ggtataaccg
    79921 tacaaacctg cctcgaactc ttcaaggaac tgttgttgca gttcagcatc aagatcggtt
    79981 ttcttcactt gatcgcggaa ctgggttaac agcgttttcg gatcgagctg tacatattgc
    80041 agcatgtccg ccacggtatc gccttcgtca gacagttcta cttctacgct accgtcaggg
    80101 aacacgaaca cgtcaaccgc ttcggtatca ccgaacaggt tgtgcatatt gccaaggatc
    80161 tcctgatatg cgccgaccat aaagaaaccg agcatcggcg gattctctgg atcgtactcc
    80221 ggcattggca ttgtcgtggc aataccatca ccatcaatat agtggtcgat agcaccgtca
    80281 gagtcacagg taatatccag cagcactgcg cgacgttccg gcacttgatc cagcccttcc
    80341 agcggcagaa ccgggaacaa ctggtcgatc ccccatgcat ccggcattga ctggaacagc
    80401 gagaaattga cgtacatttt gtccgccata cgttcctgca gttcgtcgat tatcggacga
    80461 tgcgcacggt tttgcggatc cagctgcttt tgcacttcat ggcacatgct cagatagagt
    80521 tgttctgccc atgcacgttc ttgcaggcta aaggtgccgg aagagtagcc gatatgaatg
    80581 tcgtgcagat ccatctggct gtcgtgtaac cattcacgca gagaacggcg agttcccggt
    80641 tcgtgcattt cctgccaggt ttcccacatg ctttgcagcg cgcgcggcgc atcttctgca
    80701 ggcgcggtcg gcaccgtgta ttcgttacgt tccacgccga tgatattaga taccagcacg
    80761 gtgtgatgcg cagtcaccgc acgacccgat tcggtgatta ccgtcggatg cggcagaccg
    80821 ttttcttcac acgcatcacc aatcgcccag atgatgttgt tggcatattc attgaggccg
    80881 tagttcaccg aacagtcgga ctgcgaacgg gtgccttcat agtccacgcc cagaccgccg
    80941 ccgacgtcga agcactgaat attgacgccc agcttgtgca gttccacata gaaacgcgcg
    81001 gattcacgaa cgcctgtcgc gatatcgcga atattcgcca tctgcgaacc gaggtggaag
    81061 tgcagcagtt gcaggctgtc gagacgcccg gcttcacgca gggtttcaac aagttgcagt
    81121 acctgagtcg cagccaggcc gaacttcgat ttttccccgc cggaggactg ccatttaccc
    81181 gagccctgcg aagccagacg tgcacgcacg cccagacgag gaacgacatt cagacgttct
    81241 gcttcatcca gcacaatggc gatttctgac atcttctcaa tgaccaggta gaccttgtgg
    81301 cccatcttct cgccaattaa tgccaggcgg atatattcgc ggtctttata accgttgcag
    81361 acgatgacgc tacgggtcat gccagcatgt gccagcaccg ccatcaactc tgctttcgag
    81421 cctgcttcca gtcccagcgg ttcgcccgaa tgaatcaggg actcaatcac gcggcggtgc
    81481 tggttaactt tgatcggata aacaaggaag taatcgccgt tatagccgta ggattcccgc
    81541 gcacgtttga acgcggcgtt aatggaacgc aaacggtgct gcaggatctg tgggaaacag
    81601 aacagtgcag gcagacgctg accctgtgct tcacgagttt tcactaactg cgcgagatcg
    81661 acgcgagctt ccgggacgtc cgggtccggg cacacgctga tgtggcccag ctcgttaacg
    81721 tcatagtagt tattgcccca ccaggcaata ttgtaagtac gcagcatctt gctggcttcc
    81781 tgggagctca ttgcaacctc ctgcatggag cgtagtacac cgtgttcgcc cgctgacgaa
    81841 ggcaaaccca tagacatgtc gtcagacata gcgaacctca aattatttta ttaagtgtaa
    81901 aacagttaac gactatcgca gcctggaact gcgataacaa cccataagca catggatttt
    81961 ccagcagtgt atgctgacgc tccgactgcg caaccggttt ctttttcata acattattaa
    82021 gcacataacc gaacgtaagt gtgacagttc ggcgaaacca cgagaaaact cttgttttta
    82081 caagagcgcc cttgttcagt cctcagtaac tgtaaccagc tcttgaatcc tgagaagcgc
    82141 cgagatgggt ataacatcgg caggtatgca aagcagagat gcagagtgcg gggaacgaat
    82201 cttcaccaga acggtgagac aggttaagca gcagacaacg gttcattatt tcgtatcacc
    82261 tccacggccg cctgttaaga cgaacccaca agccaaaact ctgatttcaa cccggctgga
    82321 agtggcaaca cgaaagaaac gtcgtgtgct ttttatttaa gccgcgcgcc gcgttttata
    82381 ccccacaatg gcagaaaatt gcaaaagata aatacgcaga atgccggcat tgtcaggaaa
    82441 aatttccagc cacgttttta acagaatgag acacgattca aaaaaaagtg gaaatcgggt
    82501 gaagaattga cctaaaatag ccatccagat gttaatccat ccataccgat taacactcag
    82561 actgccagtg tttttaactt gcagagtcgt ggtaggatcc gctaccacag aaaatccaca
    82621 caacagtttg agctaaccaa attctcttta ggtgatatta aatatggcaa aacacctttt
    82681 tacgtccgag tccgtctctg aagggcatcc tgacaaaatt gctgaccaaa tttctgatgc
    82741 cgttttagac gcgatcctcg aacaggatcc gaaagcacgc gttgcttgcg aaacctacgt
    82801 aaaaaccggc atggttttag ttggcggcga aatcaccact agcgcctggg tagacatcga
    82861 agagatcacc cgtaacaccg ttcgcgaaat tggctatgta cattccgaca tgggctttga
    82921 cgctaactcc tgtgcggttc tgagcgctat cggcaaacag tctcctgaca tcaaccaggg
    82981 cgttgaccgt gccgatccgc tggaacaggg cgcgggtgac cagggtctga tgtttggcta
    83041 cgcaactaat gaaaccgacg tgctgatgcc agcacctatc acctatgcac accgtctggt
    83101 acagcgtcag gctgaagtgc gtaaaaacgg cactctgccg tggctgcgcc cggacgcgaa
    83161 aagccaggtg actttccagt atgacgacgg caaaatcgtt ggtatcgatg ctgtcgtgct
    83221 ttccactcag cactctgaag agatcgacca gaaatcgctg caagaagcgg taatggaaga
    83281 gatcatcaag ccgattctgc ccgctgaatg gctgacttct gccaccaaat tcttcatcaa
    83341 cccgaccggt cgttttgtta tcggtggccc gatgggtgac tgcggtctga ctggtcgtaa
    83401 aattatcgtt gatacctacg gcggcatggc acgtcacggt ggcggagcat tctctggtaa
    83461 agatccatca aaagtggacc gttccgctgc gtacgcagca cgttatgttg caaaaaacat
    83521 cgttgctgct ggcctggccg atcgttgtga aattcaggtt tcctacgcaa tcggcgtggc
    83581 tgaaccgacc tccatcatgg tagaaacttt cggtaccgag aaagtgcctt ctgaacaact
    83641 gactctgctg gtacgtgagt tcttcgacct gcgcccatac ggtctgattc agatgctgga
    83701 tctgctgcac ccgatctaca aagaaaccgc agcatacggt cactttggtc gtgaacattt
    83761 cccgtgggaa aaaaccgaca aagcgcagct gctgcgcgat gctgccggtc tgaagtaatc
    83821 tttcttcacc tgctttcaaa ggccagcctc gcgctggcct ttttctttta cctcaccgcg
    83881 gtggataggc attcacgccg catccggcaa aaagctgccc gcacaataac atcattcttc
    83941 ctcaacacgt tttatcgcaa attatcatca ctactgaaac cgattacacc aataaca
//
LOCUS       DAZKGS010000004        81740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKGS010000004 DAZKGS010000000
VERSION     DAZKGS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHOQY_000278"
     CDS             complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHOQY_000278"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9676440.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHOQY_000279"
     CDS             complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHOQY_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9676441.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHOQY_000280"
     CDS             complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHOQY_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9676442.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHOQY_000281"
     CDS             complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHOQY_000281"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9676443.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHOQY_000282"
     CDS             complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHOQY_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9676444.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHOQY_000283"
     CDS             complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHOQY_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9676445.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHOQY_000284"
     CDS             complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHOQY_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9676446.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHOQY_000285"
     CDS             complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHOQY_000285"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9676447.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHOQY_000286"
     CDS             complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHOQY_000286"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9676448.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHOQY_000287"
     CDS             complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHOQY_000287"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9676449.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHOQY_000288"
     CDS             complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHOQY_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9676450.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHOQY_000289"
     CDS             complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHOQY_000289"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9676451.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHOQY_000290"
     CDS             complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHOQY_000290"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9676452.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHOQY_000291"
     CDS             complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHOQY_000291"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9676453.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHOQY_000292"
     CDS             complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHOQY_000292"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9676454.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHOQY_000293"
     CDS             complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHOQY_000293"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9676455.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHOQY_000294"
     CDS             complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHOQY_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9676456.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHOQY_000295"
     CDS             complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHOQY_000295"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9676457.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHOQY_000296"
     CDS             complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHOQY_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9676458.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHOQY_000297"
     CDS             complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHOQY_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9676459.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23548..23634)
                     /locus_tag="ACHOQY_000298"
     CDS             complement(23548..23634)
                     /locus_tag="ACHOQY_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9676460.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHOQY_000299"
     CDS             complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHOQY_000299"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9676461.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHOQY_000300"
     CDS             complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHOQY_000300"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9676462.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25983..26027
                     /locus_tag="ACHOQY_000301"
     CDS             25983..26027
                     /locus_tag="ACHOQY_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676463.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHOQY_000302"
     CDS             complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHOQY_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9676464.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            27786..27872
                     /gene="leuL"
                     /locus_tag="ACHOQY_000303"
     CDS             27786..27872
                     /gene="leuL"
                     /locus_tag="ACHOQY_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9676465.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            27965..29536
                     /gene="leuA"
                     /locus_tag="ACHOQY_000304"
     CDS             27965..29536
                     /gene="leuA"
                     /locus_tag="ACHOQY_000304"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9676466.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            29536..30627
                     /gene="leuB"
                     /locus_tag="ACHOQY_000305"
     CDS             29536..30627
                     /gene="leuB"
                     /locus_tag="ACHOQY_000305"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9676467.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            30630..32030
                     /gene="leuC"
                     /locus_tag="ACHOQY_000306"
     CDS             30630..32030
                     /gene="leuC"
                     /locus_tag="ACHOQY_000306"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9676468.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            32041..32646
                     /gene="leuD"
                     /locus_tag="ACHOQY_000307"
     CDS             32041..32646
                     /gene="leuD"
                     /locus_tag="ACHOQY_000307"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9676469.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(32695..33873)
                     /gene="setA"
                     /locus_tag="ACHOQY_000308"
     CDS             complement(32695..33873)
                     /gene="setA"
                     /locus_tag="ACHOQY_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFP9676470.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(33975..34106)
                     /gene="sgrT"
                     /locus_tag="ACHOQY_000309"
     CDS             complement(33975..34106)
                     /gene="sgrT"
                     /locus_tag="ACHOQY_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9676471.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            34195..35850
                     /gene="sgrR"
                     /locus_tag="ACHOQY_000310"
     CDS             34195..35850
                     /gene="sgrR"
                     /locus_tag="ACHOQY_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9676472.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      35884..35983
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            36014..36997
                     /gene="thiB"
                     /locus_tag="ACHOQY_000311"
     CDS             36014..36997
                     /gene="thiB"
                     /locus_tag="ACHOQY_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9676473.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDLRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            36973..38583
                     /gene="thiP"
                     /locus_tag="ACHOQY_000312"
     CDS             36973..38583
                     /gene="thiP"
                     /locus_tag="ACHOQY_000312"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9676474.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            38567..39265
                     /gene="thiQ"
                     /locus_tag="ACHOQY_000313"
     CDS             38567..39265
                     /gene="thiQ"
                     /locus_tag="ACHOQY_000313"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9676475.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(39379..40143)
                     /gene="yabI"
                     /locus_tag="ACHOQY_000314"
     CDS             complement(39379..40143)
                     /gene="yabI"
                     /locus_tag="ACHOQY_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9676476.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(40229..41107)
                     /gene="araC"
                     /locus_tag="ACHOQY_000315"
     CDS             complement(40229..41107)
                     /gene="araC"
                     /locus_tag="ACHOQY_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9676477.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            41446..43146
                     /gene="araB"
                     /locus_tag="ACHOQY_000316"
     CDS             41446..43146
                     /gene="araB"
                     /locus_tag="ACHOQY_000316"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9676478.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            43157..44659
                     /gene="araA"
                     /locus_tag="ACHOQY_000317"
     CDS             43157..44659
                     /gene="araA"
                     /locus_tag="ACHOQY_000317"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9676479.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            44828..45523
                     /gene="araD"
                     /locus_tag="ACHOQY_000318"
     CDS             44828..45523
                     /gene="araD"
                     /locus_tag="ACHOQY_000318"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9676480.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            45598..47949
                     /gene="polB"
                     /locus_tag="ACHOQY_000319"
     CDS             45598..47949
                     /gene="polB"
                     /locus_tag="ACHOQY_000319"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9676481.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            48114..51020
                     /gene="rapA"
                     /locus_tag="ACHOQY_000320"
     CDS             48114..51020
                     /gene="rapA"
                     /locus_tag="ACHOQY_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9676482.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            51032..51691
                     /gene="rluA"
                     /locus_tag="ACHOQY_000321"
     CDS             51032..51691
                     /gene="rluA"
                     /locus_tag="ACHOQY_000321"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9676483.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(51808..52623)
                     /gene="djlA"
                     /locus_tag="ACHOQY_000322"
     CDS             complement(51808..52623)
                     /gene="djlA"
                     /locus_tag="ACHOQY_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9676484.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            52878..55232
                     /gene="lptD"
                     /locus_tag="ACHOQY_000323"
     CDS             52878..55232
                     /gene="lptD"
                     /locus_tag="ACHOQY_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9676485.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            55285..56571
                     /gene="surA"
                     /locus_tag="ACHOQY_000324"
     CDS             55285..56571
                     /gene="surA"
                     /locus_tag="ACHOQY_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9676486.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            56571..57560
                     /gene="pdxA"
                     /locus_tag="ACHOQY_000325"
     CDS             56571..57560
                     /gene="pdxA"
                     /locus_tag="ACHOQY_000325"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9676487.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            57557..58378
                     /gene="rsmA"
                     /locus_tag="ACHOQY_000326"
     CDS             57557..58378
                     /gene="rsmA"
                     /locus_tag="ACHOQY_000326"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9676488.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            58381..58758
                     /gene="apaG"
                     /locus_tag="ACHOQY_000327"
     CDS             58381..58758
                     /gene="apaG"
                     /locus_tag="ACHOQY_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9676489.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            58765..59613
                     /gene="apaH"
                     /locus_tag="ACHOQY_000328"
     CDS             58765..59613
                     /gene="apaH"
                     /locus_tag="ACHOQY_000328"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9676490.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            59638..60237
                     /locus_tag="ACHOQY_000329"
     CDS             59638..60237
                     /locus_tag="ACHOQY_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFP9676491.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            60273..60740
                     /locus_tag="ACHOQY_000330"
     CDS             60273..60740
                     /locus_tag="ACHOQY_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676492.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(60839..61018)
                     /locus_tag="ACHOQY_000331"
     CDS             complement(60839..61018)
                     /locus_tag="ACHOQY_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP9676493.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(61039..61518)
                     /gene="folA"
                     /locus_tag="ACHOQY_000332"
     CDS             complement(61039..61518)
                     /gene="folA"
                     /locus_tag="ACHOQY_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9676494.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(61710..63572)
                     /gene="kefC"
                     /locus_tag="ACHOQY_000333"
     CDS             complement(61710..63572)
                     /gene="kefC"
                     /locus_tag="ACHOQY_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9676495.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANMLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(63565..64095)
                     /gene="kefF"
                     /locus_tag="ACHOQY_000334"
     CDS             complement(63565..64095)
                     /gene="kefF"
                     /locus_tag="ACHOQY_000334"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9676496.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(64203..65534)
                     /gene="yaaU"
                     /locus_tag="ACHOQY_000335"
     CDS             complement(64203..65534)
                     /gene="yaaU"
                     /locus_tag="ACHOQY_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9676497.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(65593..65880)
                     /gene="fixX"
                     /locus_tag="ACHOQY_000336"
     CDS             complement(65593..65880)
                     /gene="fixX"
                     /locus_tag="ACHOQY_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9676498.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(65877..67163)
                     /gene="fixC"
                     /locus_tag="ACHOQY_000337"
     CDS             complement(65877..67163)
                     /gene="fixC"
                     /locus_tag="ACHOQY_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9676499.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(67214..68155)
                     /gene="fixB"
                     /locus_tag="ACHOQY_000338"
     CDS             complement(67214..68155)
                     /gene="fixB"
                     /locus_tag="ACHOQY_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9676500.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(68170..68940)
                     /gene="fixA"
                     /locus_tag="ACHOQY_000339"
     CDS             complement(68170..68940)
                     /gene="fixA"
                     /locus_tag="ACHOQY_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9676501.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            69414..70928
                     /gene="caiT"
                     /locus_tag="ACHOQY_000340"
     CDS             69414..70928
                     /gene="caiT"
                     /locus_tag="ACHOQY_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9676502.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            70959..72101
                     /gene="caiA"
                     /locus_tag="ACHOQY_000341"
     CDS             70959..72101
                     /gene="caiA"
                     /locus_tag="ACHOQY_000341"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9676503.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            72230..73447
                     /gene="caiB"
                     /locus_tag="ACHOQY_000342"
     CDS             72230..73447
                     /gene="caiB"
                     /locus_tag="ACHOQY_000342"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9676504.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            73521..75074
                     /gene="caiC"
                     /locus_tag="ACHOQY_000343"
     CDS             73521..75074
                     /gene="caiC"
                     /locus_tag="ACHOQY_000343"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9676505.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            75183..75968
                     /gene="caiD"
                     /locus_tag="ACHOQY_000344"
     CDS             75183..75968
                     /gene="caiD"
                     /locus_tag="ACHOQY_000344"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9676506.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            75974..76564
                     /gene="caiE"
                     /locus_tag="ACHOQY_000345"
     CDS             75974..76564
                     /gene="caiE"
                     /locus_tag="ACHOQY_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9676507.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(76650..77045)
                     /gene="caiF"
                     /locus_tag="ACHOQY_000346"
     CDS             complement(76650..77045)
                     /gene="caiF"
                     /locus_tag="ACHOQY_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9676508.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(77306..80527)
                     /gene="carB"
                     /locus_tag="ACHOQY_000347"
     CDS             complement(77306..80527)
                     /gene="carB"
                     /locus_tag="ACHOQY_000347"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9676509.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(80545..81693)
                     /gene="carA"
                     /locus_tag="ACHOQY_000348"
     CDS             complement(80545..81693)
                     /gene="carA"
                     /locus_tag="ACHOQY_000348"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9676510.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        19649 a        22510 c        20767 g        18814 t
ORIGIN      
        1 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
       61 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
      121 aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
      181 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
      241 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
      301 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
      361 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
      421 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
      481 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
      541 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
      601 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
      661 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
      721 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
      781 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
      841 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
      901 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
      961 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
     1021 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
     1081 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
     1141 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
     1201 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
     1261 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
     1321 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
     1381 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
     1441 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
     1501 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
     1561 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
     1621 tctacggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
     1681 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
     1741 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
     1801 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
     1861 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
     1921 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
     1981 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
     2041 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
     2101 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
     2161 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
     2221 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
     2281 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
     2341 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
     2401 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
     2461 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
     2521 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
     2581 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
     2641 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
     2701 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
     2761 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
     2821 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
     2881 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
     2941 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
     3001 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
     3061 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
     3121 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
     3181 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
     3241 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
     3301 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
     3361 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
     3421 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
     3481 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
     3541 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
     3601 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
     3661 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
     3721 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
     3781 tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac aaatcttatc
     3841 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
     3901 aacagcgttc gcaccgtttt ttatagctct tataaaattt aaaaaaaaac gactggttca
     3961 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     4021 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     4081 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     4141 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     4201 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     4261 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     4321 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     4381 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     4441 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
     4501 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
     4561 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     4621 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     4681 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     4741 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     4801 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     4861 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     4921 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
     4981 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     5041 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     5101 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     5161 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     5221 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
     5281 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
     5341 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
     5401 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
     5461 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
     5521 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     5581 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     5641 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
     5701 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     5761 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     5821 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
     5881 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
     5941 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     6001 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     6061 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
     6121 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     6181 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     6241 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     6301 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
     6361 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
     6421 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
     6481 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
     6541 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
     6601 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
     6661 cggacgacca cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
     6721 cgcacaaccg tggcgaactt taatcgcttc ggcatcgctt ggcggcgtgc caaaggcgta
     6781 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
     6841 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
     6901 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
     6961 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
     7021 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
     7081 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
     7141 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
     7201 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
     7261 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
     7321 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
     7381 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
     7441 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
     7501 cgccttgatc attgttgttc tgcctgtgca tgattttgtt gctgagtaga ttcctctggc
     7561 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
     7621 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
     7681 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
     7741 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
     7801 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
     7861 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
     7921 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
     7981 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
     8041 tctatttgcg tctggatgat gttgacatct tgggtcataa aggtacccgg ctcacccaat
     8101 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
     8161 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
     8221 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
     8281 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
     8341 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
     8401 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
     8461 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
     8521 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
     8581 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
     8641 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
     8701 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
     8761 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
     8821 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
     8881 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
     8941 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
     9001 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
     9061 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
     9121 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
     9181 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
     9241 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
     9301 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
     9361 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
     9421 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
     9481 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
     9541 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
     9601 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
     9661 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
     9721 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
     9781 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
     9841 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
     9901 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
     9961 cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    10021 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    10081 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    10141 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    10201 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    10261 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    10321 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    10381 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    10441 cgacaatttc cgggttatag gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    10501 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    10561 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    10621 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    10681 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    10741 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggctgcccg
    10801 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    10861 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    10921 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    10981 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    11041 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    11101 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    11161 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    11221 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    11281 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    11341 cggcagcgcc agtacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    11401 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    11461 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    11521 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    11581 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    11641 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    11701 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    11761 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    11821 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    11881 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    11941 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    12001 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    12061 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    12121 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    12181 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    12241 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    12301 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    12361 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    12421 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    12481 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    12541 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    12601 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    12661 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    12721 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    12781 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    12841 cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag
    12901 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    12961 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    13021 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    13081 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    13141 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    13201 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    13261 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    13321 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    13381 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    13441 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    13501 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    13561 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    13621 tcatctcttt cacgttcagc actttctcgc ctttcactcg cagccaggtt tctccctgct
    13681 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatcagcac
    13741 cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc gcattttcgt
    13801 aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg tccatatgat
    13861 cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct
    13921 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    13981 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    14041 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    14101 cttcgcggca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    14161 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    14221 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    14281 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    14341 ccacgcagga aagcccagtg aggcccaggc cgataatgac gacattttta ccctgataat
    14401 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    14461 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    14521 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    14581 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    14641 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    14701 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    14761 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    14821 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    14881 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    14941 aatgacgaag taagccagca gaatgtagaa cagccccagc tgcggcatca catctttaaa
    15001 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    15061 gatacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    15121 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    15181 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    15241 cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    15301 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    15361 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    15421 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    15481 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    15541 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    15601 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    15661 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    15721 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    15781 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    15841 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    15901 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    15961 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    16021 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    16081 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    16141 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    16201 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    16261 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    16321 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    16381 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    16441 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    16501 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    16561 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    16621 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    16681 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    16741 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    16801 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    16861 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    16921 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    16981 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    17041 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    17101 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    17161 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    17221 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    17281 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttta
    17341 acagatcagc cagcgggtag ccaagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    17401 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgaactaa
    17461 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    17521 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    17581 gccagcggcg gcccacttca tcgtcggcgt taacaatcgc ctgaccacaa tgatgttcag
    17641 aataaagcag ccatttcgcg gcttcgtagt gttccatatc accatgataa tcaaggtgat
    17701 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    17761 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgatcc accagccccg
    17821 ccagctcatg ctgaacatcg actgccgaac cggtggtatt ttctgtcggg atcactttcc
    17881 ccagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    17941 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    18001 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    18061 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    18121 cacctttcgc ctctgcaata atggcagtca caccttgcgc tatcgcctgc gggatatatc
    18181 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    18241 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    18301 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    18361 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    18421 atgatggcac caaagaccgg cgctgaaacg gcgccgccgt agtatttacc cgcctgcgga
    18481 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    18541 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    18601 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    18661 acgctttcca tcatatgcac cacagtgcgg acaatggatt ccgggaagac acgttcaccg
    18721 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    18781 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    18841 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    18901 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    18961 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    19021 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    19081 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    19141 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    19201 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    19261 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    19321 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    19381 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    19441 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    19501 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    19561 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    19621 agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaagcgc
    19681 cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta
    19741 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    19801 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    19861 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    19921 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    19981 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    20041 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    20101 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    20161 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    20221 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    20281 cgggtatggt gcgccgtggt taccacagtc accgccgtca aaataatgca aatgaacagg
    20341 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    20401 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    20461 atgcattcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    20521 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    20581 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    20641 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    20701 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    20761 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    20821 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    20881 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    20941 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    21001 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    21061 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    21121 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    21181 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    21241 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    21301 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    21361 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    21421 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    21481 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    21541 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    21601 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    21661 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg tagggggtaa
    21721 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    21781 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    21841 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    21901 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    21961 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    22021 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    22081 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    22141 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    22201 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    22261 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    22321 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    22381 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    22441 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcggcgat agagattacg
    22501 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    22561 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgcaagca ccgggcactg
    22621 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    22681 accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    22741 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    22801 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    22861 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    22921 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    22981 ggcaccaacc acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg
    23041 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    23101 aataatggca tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg
    23161 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgctcaag
    23221 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    23281 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    23341 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    23401 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    23461 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    23521 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    23581 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    23641 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    23701 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    23761 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    23821 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    23881 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    23941 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    24001 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    24061 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    24121 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    24181 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    24241 acaccaggcg attattgcac acctgttcca gcgcctcgct aagtttgctt tccagctcat
    24301 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    24361 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    24421 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    24481 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    24541 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    24601 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagtgcag
    24661 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    24721 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    24781 actgacgagc gcgccactgt tcaatttgct gccaccagtc gcggatctca tccagtggtt
    24841 gatgggcgga ttcttgcgac aagagttcaa gcatttgttc gaggacctgg cgagcatccc
    24901 ccacaatcgg gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat
    24961 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
    25021 gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    25081 cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccagcccca
    25141 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    25201 agcccgccat gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg
    25261 tttgcagagc gcgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
    25321 tactgaccga ctcaggccag acatagggta atttgttcgc cggattaaga atatctttcg
    25381 gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca
    25441 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    25501 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    25561 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    25621 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    25681 ccagaccatc ggccatatgc actgccgcct gctcatgacg aactaaaaca tgatcgatac
    25741 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    25801 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    25861 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    25921 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
    25981 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct
    26041 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa cagagagccc
    26101 acacattcag aataaaattc tacattcaat aaaaaataca gaataaacca gacattcatg
    26161 cctgacctat tctgcattca gttagcgttt gcaaattgag actaattgct cttccatcca
    26221 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctatt
    26281 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
    26341 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
    26401 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
    26461 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    26521 cagtaacggg cccttaattg ttggatgatt tttgctggct accagcacca tttcatcttt
    26581 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    26641 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    26701 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    26761 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
    26821 accaggcaat tcattttgta ctagttgcaa tgcctgacga actgagccaa aaagttgaaa
    26881 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
    26941 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
    27001 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    27061 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
    27121 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    27181 taatcctgaa gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa
    27241 ttcggaaaac ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt
    27301 catttaccac agcataataa tccataatgt ttttgttgcg aaaacaatct aatcatgact
    27361 accgcgaata ctcaatcatc tacaaaatgg attaaatgtg cttatttaat aattaattta
    27421 tatatttaat gcattaattc ttaacattaa ttgatcaata atattcacca aatcaatatc
    27481 aaaaaaaatc gcaaaacata taattcaata caaatcatca ggataggttt tgcaacgcgt
    27541 gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa tttatgctga aatatgtcaa
    27601 ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg cttactttaa ccaccgcagc
    27661 acaattagct aattttacgg gtgcagaact cacgctggcg ggacgttttt attgcgtcag
    27721 ggttgacatc cgtttttgta tccagtaact ctaaaagcat atcgcattca tctggagctg
    27781 atttaatgac tcacatcgtt cgctttatcg gtctactact actaaacgca tcttctttgc
    27841 gcggtagacg agtgagcggc atccagcatt aagccagcac gcagtcaaac aaaaaacccg
    27901 cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct ctaaaagaga caaggaccca
    27961 aaccatgagc cagcaagtca ttattttcga taccacattg cgcgacggtg aacaggcgtt
    28021 acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg ctggcccttg agcgtatggg
    28081 tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg ggcgattttg aatcggtgca
    28141 aaccatcgcc cgccaggtta aaaacagccg cgtatgtgcg ttagctcgct gcgtggaaaa
    28201 agatatcgac gtggcggccg aatccctgaa agtcgccgaa gccttccgta ttcatacctt
    28261 tattgctacc tccccaatgc acatcgccac caagctgcgc agcaccctgg acgaggtgat
    28321 cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac accgatgatg ttgaattttc
    28381 ttgcgaagat gccgggcgta cacccattgc cgatctggcg cgagtggtcg aagcggcgat
    28441 taatgccggt gccaccacca tcaacattcc ggacaccgtg ggctacacca tgccgtttga
    28501 gttcgccgga atcatcagcg gcctgtatga acgcgtacct aacatcgaca aagccattat
    28561 ttccgtacat acccacgacg atttaggcct ggcggtcggc aactcactgg cggcggtaca
    28621 tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc ggcgagcgtg ccggtaactg
    28681 ttcgctggaa gaagtcatca tggcgattaa agttcgtaag gatattctca acgtccacac
    28741 cgccattaat caccaggaaa tatggcgcac cagccagtta gttagccaga tttgtaatat
    28801 gccgatcccg gcaaacaaag ccattgttgg cagcggcgca ttcgcacact cctccggtat
    28861 ccaccaggat ggcgtgctga aaaaccgcga aaactacgaa atcatgacac cagaatctat
    28921 tggtctgaac caaatccagc tgaatctgac ctctcgttcg gggcgtgcgg cggtgaaaca
    28981 tcgcatggac gagatggggt ataaagaaag tgaatataat ttagacaatt tgtacgacgc
    29041 cttcctgaag ctggcggaca aaaaaggtca ggtgtttgat tacgatctgg aggcgctggc
    29101 cttcatcggt aagcagcaag aagagccgga gcatttccgt ctggattact tcagcgtgca
    29161 gtctggctct aacgatatcg ccaccgccgc cgtcaaactg gcctgtggcg aagaagtcaa
    29221 agcagaagcc gccaacggta acggtccggt cgatgccgtc tatcaggcaa ttaaccgcat
    29281 cactgactat aacgtcgaac tggtgaaata cagcctgacc gccaaaggtc acggtaaaga
    29341 tgcgctgggt caggtggata ttgtcgccaa ctacaacggt cgccgcttcc acggcgtcgg
    29401 cctggccacc gatattgtcg agtcctccgc caaagccatg gtgcacgtac tgaacaatat
    29461 ctggcgtgcc gcagaagtcg aaaaagagtt gcaacgcaaa gctcaacaca acgaaaacaa
    29521 caaggaaacc gtgtgatgtc gaagaattac catattgccg tattgccggg ggacggtatt
    29581 ggtccggaag tgatgaccca ggcgctgaaa gtgctggatg ccgtgcgcaa ccgctttgcg
    29641 atgcgcatca ccaccagcca ttacgatgta ggcggtgcag ccattgataa ccacgggcaa
    29701 ccactgccgc ctgcgacggt tgaaggttgt gagcaagccg atgccgtgct gtttggctcg
    29761 gtaggcggtc cgaaatggga acatttacca ccagaccagc aaccagaacg cggcgcgctg
    29821 ttgcctttgc gtaagcactt caaattattc agcaacctgc gcccggcaaa actgtatcag
    29881 gggctggaag cattctgtcc gctgcgtgca gacattgccg ctaacggctt cgacatcctg
    29941 tgcgtgcgcg aactgaccgg cggcatctat ttcggtcagc caaaaggccg cgaaggtagc
    30001 ggacaatatg aaaaagcgtt tgataccgag gtgtatcacc gttttgagat cgagcgtatt
    30061 gcccgcatcg cgtttgaatc tgctcgtaaa cgtcgccaca aagtcacctc aatcgacaaa
    30121 gctaacgtgc tgcaatcctc tattttatgg cgtgaaatcg tcaacgagat cgccacggaa
    30181 tacccggatg tcgaactggc gcatatgtac atcgacaacg ccaccatgca gctgattaaa
    30241 gatccatcac agtttgacgt tctgctgtgc tccaacctgt ttggcgacat tctgtctgac
    30301 gagtgcgcaa tgatcactgg ctcgatgggg atgttgcctt ccgccagcct gaacgagcaa
    30361 ggttttggtc tgtatgaacc ggcaggcggc tcagcaccag atatcgcagg caaaaacatc
    30421 gccaacccga ttgcgcaaat tctgtcgctg gcactgctgc tgcgctacag cctggatgcc
    30481 gatgatgcgg cttgcgccat tgaacgcgcc attaaccgcg cattagaaga aggcattcgc
    30541 accggggatt tagcccgtgg cgctgccgcc gttagtaccg atgaaatggg cgatatcatt
    30601 gcccgctatg tggcagaagg ggtgtaatca tggctaaaac gttatacgaa aaattattcg
    30661 acgctcacgt agtgtacgaa gccgaaaacg aaaccccgct gttatatatc gaccgccacc
    30721 tggtgcatga agtgacctca ccgcaggcgt tcgatggtct gcgcgcccac ggtcgcccgg
    30781 tacgtcagcc gggcaaaacc ttcgccacta tggatcacaa cgtctctacc cagacgaaag
    30841 acattaatgc ctgtggtgaa atggcgcgca tccagatgca ggagctgatt aaaaactgca
    30901 aagaatttgg cgtcgaactg tatgacctga atcacccgta tcaggggatc gtccacgtaa
    30961 tggggccgga acagggcgtc accttgccgg ggatgaccat tgtctgcggc gactcgcata
    31021 ccgccaccca cggcgcgttt ggcgcactgg cctttggtat cggcacttcc gaagttgaac
    31081 acgtactggc aacgcaaacc ctgaaacagg gccgcgcaaa aaccatgaaa attgaagtcc
    31141 agggcaaagc cgcgccgggc attaccgcaa aagatatcgt gctggcaatt atcggtaaaa
    31201 ccggtagcgc aggcggcacc gggcatgtgg tggagttttg cggcgaagca atccgtgatt
    31261 taagcatgga aggtcgtatg accctgtgca atatggcaat cgaaatgggc gcaaaagccg
    31321 gtctggttgc accggacgaa accaccttta actatgtcaa aggccgtttg catgcgccga
    31381 aaggcaaaga tttcgacgac gccgttgcct actggaaaac cctgcaaacc gacgaaggcg
    31441 caactttcga taccgttgtc actctgcaag cagaagaaat ttcaccgcag gtcacctggg
    31501 gcaccaatcc aggccaggtg atttccgtga acgacaatat tcccgatccg gcttcgtttg
    31561 ccgatccggt tgaacgcgcg tcggcagaaa aagcgctggc ctatatgggg ctgaaaccgg
    31621 gcattccgct gaccgaagtg gctatcgaca aagtgtttat cggttcctgt accaactcgc
    31681 gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg gcgaaaagtc gcgccaggcg
    31741 tgcaggcact ggtggttccc ggctctggcc cggtaaaagc ccaggcggaa gcggaaggtc
    31801 tggataaaat ctttattgaa gccggttttg aatggcgctt gcctggctgc tcaatgtgtc
    31861 tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg tgcatccacc agcaaccgta
    31921 actttgaagg ccgccagggg cgcggcgggc gcacgcatct ggtcagcccg gcaatggctg
    31981 ccgctgctgc agtgaccgga catttcgccg acattcgcaa cattaaataa ggagcacacc
    32041 atggcagaga aatttatcaa acacacaggc ctggtggttc cgctggatgc cgccaatgtc
    32101 gataccgatg caatcatccc gaaacagttt ttgcagaaag tgacccgtac gggttttggc
    32161 gcgcatctgt ttaacgactg gcgttttctc gatgaaaaag gccaacagcc aaacccggac
    32221 ttcgtgctga acttcccgca gtatcagggc gcttccattt tgctggcacg agaaaacttc
    32281 ggctgtggct cctcgcgtga gcacgcgccc tgggcattga ccgactacgg ttttaaagtg
    32341 gtgattgcgc cgagttttgc tgacatcttc tacggcaata gctttaacaa ccagctgctg
    32401 ccggtgaaat taagcgatgc agaagtggac gaactgtttg cgctggtgaa agccaatccg
    32461 gggatccatt tcgacgtgga tctggaagcg caagaggtga aagcgggaga gaaaacctat
    32521 cgctttacca tcgatgcctt ccgccgccac tgcatgatga acggtctgga cagtattggg
    32581 cttaccttgc agcacgacga cgccattgcc gcttatgaag caaaacaacc tgcgtttatg
    32641 aattaatccc cttgcccggt caaatgaccg ggctttccgc tatcgtccac gtcatcaaac
    32701 gtctttaacc tttgcggtta aaaataatgc gacaacagaa ataaccgcaa ttacccagta
    32761 gacagcaaag tgcccccaac tttgtgcaat tgctccctga ataacgccag ccagaattac
    32821 cccggtagaa atactgttag taaataaggt ggtagctgcc cccgctcttc caggcattaa
    32881 atcctgaaac catagcatcc caatacccgc aacaatgccg ataaatacag cgttaaaaag
    32941 ttgcagcgtc atcaacgcca tacggctatg aaagaaaatc aatccggtgt aaaacagtac
    33001 tcctgccgcc actgctatga ccatcattcg ccgcttacca taacgtttga catagtagcc
    33061 agccagaatc attgctggta tttccagtcc agctgccgtc cccatcagga aacccgccag
    33121 tttgtctggc aatcctaact cgctactgat ccacaacggc atatcaataa tgtacatggt
    33181 gttgcaggtc cacattaacg tcgaggcgac aaataacatc cgtacgttac tatcctgcca
    33241 gccaccttgc attgataaag cattttccga cggcagttct acccgcgcca cagacggaag
    33301 cataaatgca atcaatacca gactgagtgt gaatatcccg gcggcaatcg aaaacatcac
    33361 cgtaaagccg taattcaacg ccagcataaa ggccaacggt ggaccgataa cccatgccag
    33421 agaaagctgc gcacgcatca ccgagctaaa catcaccact tctcgcgccg agttatccgc
    33481 atattcccgc gccagagcaa ataactgtgg cattgccgta ttggccagag atgccagaag
    33541 cacaccacag gtgataagcg tcagataatg acgattaaat gcaaacaata gcgcattgcc
    33601 gatagccatc aaacagcaaa atataatcag ttttcgccga tcgccctgac tgtcagaacg
    33661 ttttgccaac cagaggctta cgccgatccc agcaatagca ttcaccgtat aaaagagtcc
    33721 gatccagaaa ggttgcgcgc caacctcacg actcagaaat aagctcaatg taggagcctg
    33781 tagcgccccg gccaccccca tcataaaagc gaccagcata aatgccgcgt aaacaccgtt
    33841 catacggcga gccatcgtca ttatccagat catacgttcc ctttttagcg cggcgagaat
    33901 aaaaaaaacc agcaggtata atctgctggc gggtgatttt acaccaatac tcagtcacac
    33961 atgatgcagg caagtcaact ttcagaattg cggtcatccc actgcatcag ttcttcaagc
    34021 atggttaatc gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta
    34081 aaatagtgct gataaaactg acgcatgggg cacctccttg cttcatcgtt aggaattatt
    34141 gacttaatat agggaaaata aaattgctgt cttttgcgca ggagttcccc ttttatgcca
    34201 tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag
    34261 gacacaacgc tcaacgaact ggcagcgtta ttgagctgct cgcgtcgtca tatgcgtacc
    34321 ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt cgggcgcggt
    34381 aaacgctcgc gtctgacatt cctctatacc gggctggcgc ttcagcaaca gcgggcggaa
    34441 gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt tggttggcga caaagcgact
    34501 gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc gccaggggcg gcacatcctg
    34561 cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc
    34621 gaaacccata ttgcccggca aatcttcagt tcgctaacgc gcataaatga ggaaaatggg
    34681 gaactggaag cagacatcgc ccaccactgg cagcaaatat caccgcttca ctggcgtttc
    34741 tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc
    34801 gcctctttaa aacgaatcaa tacgctgccg ctctattcgc atattgcgga cattgtgtcg
    34861 ccgacgccct ggacgctgga tatccatctc acgcaaccgg accgctggtt accgttactg
    34921 ctgggacaag ttccggcgat gatcctgccg cgcgaatggg aaaccctcag taactttgcc
    34981 agccatccca tcggcaccgg tccgtatgcg gtgatccgca acaccaccaa tcaactgaaa
    35041 attcaggcat tcgatgactt cttcggttac cgggcattaa tcgatgaagt taacgtctgg
    35101 gttctgccgg aaattgccga cgagccagct ggaggtctga tgctaaaagg tccacagggt
    35161 gaggaaaaag agattgaaag ccgcctggag gaaggttgct actatttact gttcgacagc
    35221 cgcacccatc gcggggcgaa tcagcaagtc agggactggg taagctatgt gctttctcca
    35281 actaatctgg tctatttcgc tgaggaacag taccagcaac tgtggttccc ggcttatgga
    35341 ctgctccccc gttggcacca tgcccgcacc ataaagagcg aaaaaccggc tggcctggaa
    35401 agcctcatcc tgacctttta tcaggatcac agtgagcatc gggtgattgc cgggatcatg
    35461 cagcagattc tggcaagtca ccaggtcacg ctggaaatca aagagatcag ctacgatcag
    35521 tggcatgaag gagagatcga gagcgatatc tggcttaaca gcgccaactt tacgctgcct
    35581 ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc tgctccaaca ctgtattccg
    35641 atcgactggc aagccgacgc cgcccgctgg cgcaatggcg aaatgaacct ggcgaactgg
    35701 tgccagcaac tggtcgccag caaagcaatg gtgccgctta tccaccactg gctgatcatt
    35761 caggggcaac gcagtatgcg cggcctgcgc atgaacaccc tcggctggtt cgattttaaa
    35821 tcagcgtggt ttgcgccacc ggatccatga ttgctggtag ataacaaaat cactacacta
    35881 acgccgttct caacggggtg ccacgcaaac gcgtgcgctg agaaaatacc cgtcgaacct
    35941 gatccggata acgccggcga agggatttga ggctccttct caagtccttt gccactcttt
    36001 tttgaggtgc aaagtgttaa aaaaatgtct tcccctgctg ttgctgtgca cagcgcccgt
    36061 tttcgctaaa cccgttctga ctgtttatac ctacgattcc ttcgccgccg actgggggcc
    36121 tggtccaaag atcaaaaaag cttttgaagc cgactgtaac tgcgaactga aactggtggc
    36181 gctggaagat ggcgtttcgc ttctcaaccg tctacggatg gaaggcaaaa atagtaaagc
    36241 cgatgtggtg ctggggctgg ataacaatct gttagacgcc gccagtaaaa ccggactatt
    36301 tgccaaaagc ggtgtggcag cggatgccgt taacgttccc ggcggctgga ataatgacac
    36361 tttcgtcccg tttgattacg gctatttcgc cttcgtctat gacaagaaca aactgaaaaa
    36421 cccgccgcaa agcctgaaag aattggttga gagcgatcag aactggcggg tgatttatca
    36481 ggatctgcgc accagtacgc cggggctggg cctgctgttg tggatgcaaa aagtctatgg
    36541 cgatgacgcc ccacaagcct ggcaaaaact ggcgaagaaa accgtcacgg tcaccaaagg
    36601 ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc gatctggtac tgagttacac
    36661 cacctctccg gcttatcaca ttctcgaaga gaaaaaagat aactacgccg ccgcgaactt
    36721 tagcgaaggt cactatctgc aggtggaagt cgccgcccgc accgctgcca gcaagcagcc
    36781 ggagctggcg caaaaattcc tccagtttat ggtttctccg gcttttcaga atgcgatccc
    36841 aaccggtaac tggatgtatc cggtggcaaa tgtcacgctg cctgccgggt ttgaacaatt
    36901 gaccaaaccg gccaccacgc tggagttcac accagctgaa gtggctgcac aacgtcaggc
    36961 atggattagc gaatggcaac gcgccgtcag ccgttaattc ccggctggtt aattccaggt
    37021 gtaagcgccg ccacgctggt ggtagcggtt gcgctcgcgg cgtttctcgc cctgtggtgg
    37081 aacgcgccgc agggtaactg ggtggcagtc tggcaggaca gctacctgtg gcatgtggtg
    37141 cgcttctcct tctggcaggc gtttctctcg gcgctactct ctgtcgtacc cgcgatattc
    37201 ctcgcccgcg ccctctatcg caggcgcttt ccgggtcggc tggcgctgtt gcgtctgtgc
    37261 gcaatgacct tgatcctccc ggtgctagtt gctgttttcg gcattcttag cgtctatggt
    37321 cgtcagggct ggctggcatc gctctgccaa tcgctcggtc tggagtggac cttttcgccc
    37381 tacggcctgc aaggcatttt gctggcgcac gtatttttta atctgccgat ggcgagccgc
    37441 ttattactcc aggcactgga aaacattccc ggcgaacaac gtcagcttgc cgcccagctt
    37501 gggatgcgcg gctggcattt tttccgcttc gtcgaatggc cgtggctacg gcgacaaatc
    37561 ccgccggttg ccgcgcttat ctttatgctc tgtttcgcca gcttcgccac cgtgctatcg
    37621 ctgggcggcg gtccgcaggc aactactatc gagctggcta tttatcaggc gctgagttac
    37681 gactacgatc ctgcccgcgc ggcgatgctg gcgctgctcc agatggtgtg ttgccttggg
    37741 ctggtgctgt tgagtcagcg actaagtaag gccattgcgc ccggtaccac gctgctgcaa
    37801 ggctggcgcg atccggacga tcgtctgcat agccgcattt gcgacacggt gttaatagtg
    37861 ctggcgctgc tgctgttgct gccaccgttg ctggcggtga tcgtcgatgg ggtaaatcgc
    37921 cagttgccgg aagtgctggc acaaccggtg ctgtggcagg cgctgtggac ctcgttgcgt
    37981 attgcgctgg cggcaggtgt attgtgcgta gtgctgacca tgatgctgct atggagcagt
    38041 cgcgaactgc gggcgcggca gaaaatgctg gcgggtcagg cgctggagat gagcggcatg
    38101 ttgatcctcg ccatgccggg gattgtgctg gctaccggct tctttttact gctcaacaac
    38161 actatcggcc tgccacaatc tgctgacggc attgtgattt tcaccaatgc gttaatggcg
    38221 atcccttatg cgctgaaagt actggaaaac ccgatgcgcg atatcaccgc ccgctacagt
    38281 atgttgtgtc agtcgctggg cattgaaggc tggtcgcgct taaaagtggt cgagctgcgc
    38341 gccctgaaac gtccactggc gcaggcgctg gcttttgcct gcgtgctgtc gattggtgat
    38401 tttggcgtag tggcgttgtt cggtaacgat gatttccgta ccctgccgtt ttatctctac
    38461 cagcaaattg gctcctatcg cagccaggac ggcgcggtca ccgcgttaat tctgctacta
    38521 ctctgttttc tgctgtttac cgtgattgaa aaactaccgg ggcgaaatgt taaaactgac
    38581 tgatatcacc tggctttatc accatttgcc gatgcgtttt agcttaacgg tagaacgcgg
    38641 cgagcaggtg gcaatcctcg ggccaagcgg cgcgggtaaa agtaccctgc tgaatttgat
    38701 tgccggtttt ctgacgccag ccagcggttc gctgactatc gacagcgttg atcacaccac
    38761 gacgccgccg tcacgccgcc cggtatcgat gctgtttcag gagaacaacc tgttcagcca
    38821 cctgacggtc gcgcagaaca tcgggctggg gcttaatccg ggattgaagc tgaacgcggc
    38881 gcagcaggag aaaatgcacg ctatcgcccg ccagatgggg attgataatt taatggcgcg
    38941 gttaccgggc gagctttccg gcggtcagcg acagcgcgtg gcgttagcgc gttgtctggt
    39001 gcgcgaacag ccgattttat tgctcgatga accgttctct gcgctcgatc cggcgttacg
    39061 tcaggagatg ctgacgctgg tgagcacgag ctgccagcag caaaaaatga cgctattgat
    39121 ggtgtcgcac agtgtggaag atgcggcgcg gatcgccaca cgctcggtgg tggtcgccga
    39181 cgggcgcatc gcctggcagg gtaagaccaa tgagttgttg agcggtaagg cgagtgcttc
    39241 ggcactattg gggattacgg gttagtatgc actgtcggat gcggcgtgaa cgccttatcc
    39301 gacctacggg tagtgcgttt tgtaggcctg ataagacgcg gtcagcgtcg catcaggcaa
    39361 cccgcacaag actatctcct aacccccaac cactttacgc aaaatatcga tatacaccgg
    39421 catcaacggg tggcgaatta acaccaccag cgccaccacg ccgatggcag aaattaacgg
    39481 cgtcagccac aacaaacggc cgcgcgacaa ataatgactt aaacgatcgg ttgctttacc
    39541 gctgcgccat aaccgccagc acagccagcc accaacccac aaaaataccg ccgtcgccag
    39601 cagcaaccat ttaaactcgc cgctctgcat tccggctgga atatcgatcg ccgcgcccgc
    39661 cagaatccca ggcaggaagt aaaacggtgg ccacagcagg cagccgataa tattcggcgt
    39721 aatgaatttt gccaccggca gatccagcat tcccgccacc attggcacca gcggacgcgt
    39781 cgggccaaca aagcgaccga ccagaatggt gaacatgctg tgttgatgca acgcatgttc
    39841 agttttatcg agtaatgctt tgtttttctt cagaaatgac cagcgatgca acggcttttt
    39901 aaaacgccag cccagccaga aggagatcca gtcgcccagc aggcaaccga cgatccctgc
    39961 cagccaggcg tgccagaaac ttaactcgcc gctgccaatc agcgctccca gccccgccat
    40021 cagcaccgta ccgggtagaa tcaaaccgac cagcgccagc gactccagaa aggccaccaa
    40081 caccaccgcc atcaatgaat acacggtaga ttgggtaata aagtgttcca gcaatgcttg
    40141 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccgtca
    40201 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
    40261 tttcttcaca accggctcgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
    40321 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
    40381 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
    40441 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
    40501 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
    40561 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
    40621 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
    40681 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
    40741 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
    40801 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggataacgac
    40861 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
    40921 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
    40981 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
    41041 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
    41101 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
    41161 gacattgccg tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc
    41221 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
    41281 gtgtctataa tcacggcaga aaagtccaca ttggttattt gcacggcgtc acactttgct
    41341 atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac
    41401 tctctactgt ttctccatac ccgttttttt ggatggagtg aaacgatggc gattgcaatt
    41461 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    41521 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    41581 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    41641 aaaaccgtgc ttgcagagct tagcgtcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    41701 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    41761 ccggagtttg ccgaaaaccc gaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    41821 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    41881 tacattggcg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    41941 caggacaccg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    42001 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    42061 cataagtcgc tatggcacga aagctggggt ggcttgccgc cagccagttt ctttgatgag
    42121 ctggacccga tcctcaatcg ccatttacct tccccgctgt tcactgacac ctggactgcc
    42181 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    42241 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    42301 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    42361 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggta
    42421 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    42481 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    42541 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    42601 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggtcgtcg cacaccgaac
    42661 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cgctccgctg
    42721 ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    42781 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    42841 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    42901 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    42961 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    43021 agcgaacagg cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    43081 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttgcgact
    43141 ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg tcattggcag
    43201 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    43261 taatgcgctg aatacggaag cgaaactgcc ctgcaaacta gtgttgaaac cgctgggcac
    43321 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    43381 tctggtggtg tggctgcaca ctttctcccc ggccaaaatg tggatcaacg gcctgaccat
    43441 gctcaacaaa ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag
    43501 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    43561 cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca
    43621 agcccatgag cgtatcggct cctggatgcg ccaggcggtc tctaaacagg atacccgtca
    43681 tctgaaagtc tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa
    43741 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt
    43801 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    43861 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    43921 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    43981 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagcg
    44041 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    44101 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    44161 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    44221 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    44281 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    44341 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca cggtgaaaac
    44401 accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct
    44461 gccaactgct tccgaagcgt ggatccttgc tggtggcgcg caccataccg tcttcagcca
    44521 tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg cacgacattg aaatcacggt
    44581 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    44641 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    44701 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
    44761 ttgttttccc cctcgcccct ttggggagag ggccggggtg aggggaaacg acctctcagg
    44821 agtcaacatg ttagaagatc tcaaacgcca ggtattagaa gccaacctgg cgctgccaaa
    44881 acacaacctg gtcacgctca catggggcaa cgtcagcgcc gttgatcgcg agcgcggcgt
    44941 ctttgtgatc aaaccttccg gcgtcgatta cagcgtcatg accgctgacg atatggtcgt
    45001 ggttagcatc gaaaccggtg aagtggttga aggtacgaaa aagccctcct ccgacacgcc
    45061 aactcaccgg ctgctctatc aggcattccc ctccattggc ggcattgtgc atacgcactc
    45121 gcgccacgcc accatctggg cgcaggcggg tcagtcgatt ccagcaaccg gcaccaccca
    45181 cgccgactat ttctacggca ccattccctg cacccgcaaa atgaccgacg cagaaatcaa
    45241 cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa acctttgaaa aacagggtat
    45301 cgatgcagcg caaatgcccg gcgttctggt ccattcccac ggcccgtttg catggggcaa
    45361 aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa gaggtcgctt atatggggat
    45421 attctgccgt cagttagcgc cgcagttacc ggatatgcag caaacgctgc tggataaaca
    45481 ctatctgcgt aagcatggcg cgaaggcata ttacgggcag taatgactgt ataaaaccac
    45541 agccaatcaa acgaaaccag gctatactca agcctggttt tttgatggat tttcagcgtg
    45601 gcgcaggcag gttttatctt aacccgacac tggcgggaca ccccgcaagg gacagaagtc
    45661 tccttctggc tggcgacgga caacgggccg ttgcaggtta cgcttgcacc gcaagagtcc
    45721 gtggcgttta ttcccgccga tcaggttccc cgcgctcagc atattttgca gggtgaacaa
    45781 ggctttcgcc tgacaccgct ggcgttaaag gattttcacc gccagccggt gtatggcctt
    45841 tactgtcgcg cccatcgcca attgatgaat tacgaaaagc gcctgcgtga aggtggcgtt
    45901 accgtctacg aggccgatgt gcgcccgcca gaacgctatc tgatggagcg gtttatcacc
    45961 tcaccggtgt gggtcgaggg tgatatgcac aatggcacta tcgttaatgc ccgtctgaaa
    46021 ccgcatcccg actatcgtcc gccgctcaag tgggtttcta tagatattga aaccacccgc
    46081 cacggtgagc tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt ttatatgctg
    46141 gggccggaga atggcgacgc ctcctcgctt gatttcgaac tggaatacgt cgccagccgc
    46201 ccgcagttgc tggaaaaact caacgcctgg tttgccaact acgatcctga tgtgatcatc
    46261 ggttggaacg tggtgcagtt cgatctgcga atgctgcaaa aacatgccga gcgttaccgt
    46321 cttccgctgc gtcttgggcg cgataatagc gagctggagt ggcgcgagca cggctttaaa
    46381 aacggcgtct tttttgccca ggctaaaggt cggctaatta tcgacggtat cgaggcgctg
    46441 aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ctgtcgctca ggagctatta
    46501 ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc
    46561 gccgaagata aacctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag
    46621 atcttccaca aaactgaaat catgccattt ttactcgaac gagcaacggt gaacggcctg
    46681 ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc atctctattt tccgcgaatg
    46741 catcgcgctg gttatgtcgc gcctaatcta ggcgaagtgc cgccgcacgc cagccctggc
    46801 ggctacgtga tggattcacg gccagggctt tatgattcag tgctggtgct ggactataaa
    46861 agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggttgaaggc
    46921 atggcgcagc ctgatccaga gcacagtacc gaaggttttc tcgatgcctg gttctcacgg
    46981 gagaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga tgaagccaaa
    47041 cgtcagggta ataaaccact ttcgcaggcg ctgaagatca ttatgaatgc cttttatggc
    47101 gtgcttggca ccaccgcctg ccgctttttc gatccgcgcc tggcatcgtc gatcaccatg
    47161 cgtggtcatc agatcatgcg gcaaaccaaa gcattgattg aagcgcaggg ctacgacgtt
    47221 atctacggag ataccgactc gacttttgtc tggctaaaag gtgcacattc ggaagaagag
    47281 gcggcgaaaa tcggtcgtgc actggtgcag cacgttaacg cctggtgggc ggaaacgctg
    47341 caaaaacaac ggctgaccag cgcattagaa ctggagtatg aaactcattt ctgccgtttt
    47401 cttatgccaa ccattcgcgg agctgatacc ggcagtaaaa agcgctacgc cgggctgatt
    47461 caggagggcg acaagcagcg gatggtgttt aaagggctgg aaaccgtgcg caccgactgg
    47521 acgccgctgg cccagcagtt tcagcaggag ctgtacctgc gtatcttccg caacgagcca
    47581 tatcaggagt acgtccgcca aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg
    47641 ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct
    47701 catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa agcgcggtcg ccccttgcaa
    47761 tatcagaatc gcggcaccat taagtacgta tggaccacca acggcccgga gccgctggac
    47821 taccaacgtt caccactgga ttacgaacac tatctgaccc gccagctaca acccgtggcg
    47881 gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg
    47941 ctattttgag caaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg
    48001 ccatccagta ccatagcgcc ctttccattc ctggacctga ataacgccac tacctcataa
    48061 gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt
    48121 ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg
    48181 tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt
    48241 acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca
    48301 gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct
    48361 atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg
    48421 atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta
    48481 tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc
    48541 cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc
    48601 atgatgttgg tcgccgccac gcgccgcgcg ttctgctggc tgacgaagtg ggtttaggga
    48661 aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg
    48721 tgctgattat cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt
    48781 tcaacctgcg ttttgcgctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt
    48841 acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca
    48901 aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc
    48961 atcacctggt gtggagcgaa gacgcgccga gccgcgaata tcaggctatt gaacaactgg
    49021 cagagcacgt gccgggcgtt ctgctgctga ccgcaacccc ggaacagttg ggaatggaaa
    49081 gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg
    49141 ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg ctggcaggta
    49201 acaaactgag caatgacgaa ctgaacatgc ttggcgagat gatcggcgag caggatatcg
    49261 agccgctgtt gcaagcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggaac
    49321 tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacccgta
    49381 acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc
    49441 agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc
    49501 gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca
    49561 cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct
    49621 ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac
    49681 tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac
    49741 gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct
    49801 cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggta atgtttgacc
    49861 tgccattcaa cccggatctg ctggagcagc gtattggtcg tctggatcgt atcggtcagg
    49921 cgcacgatat tcagatccat gtgccttatc tggaaaaaac tgctcagtcg gtgctggtgc
    49981 gctggtatca cgaaggtctg gatgcattcg agcacacctg cccgaccgga cgcactattt
    50041 acgatagcgt ctacaacgat ctgattaact atctggcttc accggatcaa accgaaggct
    50101 ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca caactggaac
    50161 aggggcgtga tcgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg
    50221 cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt
    50281 tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgaccccgt
    50341 ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct
    50401 ttgatcgtga agtggcgctg gcgcgtgaag atgctcagtt tattacctgg gaacatccgc
    50461 tgatccgcaa cggtctggat ctgattcttt ctggcgatac cggtagcagc acgatttcac
    50521 tgttaaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt tacgtggtcg
    50581 aagcccaggc gccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta
    50641 tgctgctgga taaaaacggc aacaacctgg cggcccaggt ggagtttgaa acctttaacc
    50701 gccagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc gtgcagcagg
    50761 atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga
    50821 ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag
    50881 cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc
    50941 gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc ctgcgtttga
    51001 tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca
    51061 ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta acaagccgag
    51121 cggtttgttg tctgtgccgg gtcgtctgga agagcacaaa gatagcgtga tgacgcgcat
    51181 tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt
    51241 gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg
    51301 cgagccgaaa aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct
    51361 ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga
    51421 aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgtggcgg ataacacggc
    51481 aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc
    51541 gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc
    51601 accacgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag
    51661 tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg
    51721 ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg
    51781 cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc
    51841 atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa
    51901 acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt
    51961 gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt
    52021 cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc
    52081 aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc
    52141 taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc
    52201 aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca
    52261 gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccactcg
    52321 gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg
    52381 gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat
    52441 cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc
    52501 cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc
    52561 ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg
    52621 catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct
    52681 tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac
    52741 ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg
    52801 tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg
    52861 aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca
    52921 ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg
    52981 tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca
    53041 atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata
    53101 tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac
    53161 caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata
    53221 accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg
    53281 tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga
    53341 aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc
    53401 cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag
    53461 ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct
    53521 atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt
    53581 acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata
    53641 tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg
    53701 aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag
    53761 ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact
    53821 ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca
    53881 gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg
    53941 caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca
    54001 agcagttcca ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag
    54061 acgttaatta ctaccagaat gatgttggtc catttgatac gcgtatttac ggccaggcag
    54121 tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga
    54181 ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg aagttgctgg
    54241 caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg
    54301 acgaatccgt taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac
    54361 gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt
    54421 tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat
    54481 ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg
    54541 ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt
    54601 ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca
    54661 taacatggga gaatgacgac aaaacgggtt cactggtgtg ggcaggcgat acttactggc
    54721 gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg
    54781 tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga
    54841 attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg
    54901 ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg ccaattgccg
    54961 atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact
    55021 ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga
    55081 agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta
    55141 acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt
    55201 cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt
    55261 gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc
    55321 atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc
    55381 aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac
    55441 gctgctcagg caaggcagca acttcctgat gacgcgacgc tgcgccacca aatcatggaa
    55501 cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc
    55561 gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat
    55621 cagatgcgca gccgtctggc ttacgatgga ctgaactaca acacctatcg taaccagatc
    55681 cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc
    55741 ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact
    55801 gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg
    55861 aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc
    55921 ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggc
    55981 tggggccgta ttcaggagtt gcccgggatc ttcgcccagg cattaagcac cgcgaagaaa
    56041 ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac
    56101 ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg
    56161 aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca gattgctgct
    56221 gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc tcaggatcca
    56281 ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt cgatccggcc
    56341 ttccgtgacg ccctgactcg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca
    56401 ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg
    56461 cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc
    56521 tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta atggttaaaa
    56581 cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc
    56641 agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta
    56701 ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc
    56761 ctgcacaacc gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg
    56821 tcactgcggg gcagttagcg attgaaaatg gacattacgt ggtggagacg ctggcgcgag
    56881 cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag
    56941 gcgtcattaa cgacgcaggc attccgttta ccggtcatac cgagtttttc gaagagcgtt
    57001 cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa
    57061 cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag
    57121 tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc
    57181 tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag
    57241 acaccattat tccggtgctc gacgagctgc gggcgcaggg gatgaaactc aacgggccgc
    57301 tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga
    57361 tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca
    57421 ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg
    57481 cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa
    57541 tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt
    57601 cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc
    57661 gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga ccgagccggt
    57721 cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct
    57781 gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt
    57841 taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc
    57901 ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga
    57961 catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa
    58021 agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga
    58081 agttccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt
    58141 tcctcatgca acgatgcctc acccggttaa agatgttcgt gtgttgagcc gcatcaccac
    58201 cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt
    58261 cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc
    58321 gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg
    58381 atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct
    58441 tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga
    58501 gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc
    58561 gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac
    58621 cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa
    58681 atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt
    58741 cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt
    58801 tacgatgaac tgttcgcatt gctgcataaa gtagaattta cccctgggaa agataccctc
    58861 tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct gcgctatgtg
    58921 aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg
    58981 gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg
    59041 ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca aatcgacgaa
    59101 gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct acagaccgcc
    59161 aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc cttctttctc
    59221 gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga
    59281 ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt gctttccaaa cggtcaactg
    59341 gatatgtaca gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg
    59401 attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa
    59461 ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca
    59521 ttaacctgcc tgcgctggga agataagcag tattttgtcc agccgtcgaa ccggcataag
    59581 gatttgggtg agggagaggc ggtcgcgtct taacgaatta ccattcaggg aggaataatg
    59641 gctgactatt ttgaaatacg tgctgattac aatcagcaca ccatcacgat ttatcaggct
    59701 tacaatgatg caatcgctga cgtcgccgta cgtgacgggc gatttggcgc acctttttca
    59761 ttcaaccgca tgacctggat taaaccctct tttatgtgga tgatggagcg tagcaactgg
    59821 ggactgaaaa aagatcaaca acacattctc gcaatccgaa tcaaacgcac cttctttgat
    59881 acattgctgg aacaagcggt gctaaccacg ccggaagccc atgtgtatcc acatgcagga
    59941 atttgggaaa cgttatttgc acaagcaaat gtgtatgttc agtgggaccc agaacgcagc
    60001 ataaatggta aaaaactgga gcaccgatca ttacagcttg gtatttctcg taacttgatc
    60061 agtcaattta acgaagacgc gattgttgct attgatgatc tgacgccgct ggtgcgaaag
    60121 tgtcataacc ttttgataaa cggcaaaaca acacaggcga aatccttcct gccgccagaa
    60181 aaaatttacc cagttagtgc agcagcgaga aaggctttgg gaatgaaaaa ttcctgagac
    60241 aaatacgcac ctattaagcg gaaaatatct aagtgcgaca aatcaacacc gggcttaata
    60301 ccagagaaat tattctggaa atgattgacg aagcagatgc ccccccgttt gctgagcagc
    60361 ggacctggca tcgcaggcga atcgcttttc tatgtgattg catcagcgtt gctgtcgact
    60421 acggcttctt ctgttctgtc tctcagcagt caataatgaa cataaccaag atggctgaag
    60481 atgtatggat aacagaaagt aaaacgaccc aactcattga tatttggcaa caacttaaac
    60541 agctttatcc gccagaaact attccctacg cccagaagtt acccactaag gaattgcttg
    60601 aactgctact ttctagtcct gacgatcttc gaataattga aattcctgac atcatcggtg
    60661 atgtttttga tgaccaaacc tggattcgat tattcaaaaa acactttgct gatgttattc
    60721 agctacgatg tgatgtgtaa caatccaaaa tatatcgcca gacgatagac caaaaagaaa
    60781 gtatttagca gatactcaac attctgactt ctgtacagaa atagcagagt ttaaaaactt
    60841 aaaacttccg gtactcatca gagaaacatc cagtttcttc tgcaaattca tttaatgccg
    60901 agatggcatc ctcgtttact tcattccact gtagagtggc ctgaaggttt agacctacgc
    60961 aatgagcgcg actcacaaaa tcgcgctcac tggtaaaatc aaacgtctta tcgttcaatt
    61021 tgtagtcctg gcaaaaaatt accgccgctc cagaatctca aagcaatagc tgtgagagtt
    61081 ctgcgcatca gcatcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc
    61141 cgggaaatgg gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca gtttctgcgc
    61201 ttttggcagg aactgctcat aaacgcgacc gccgccaatc accatgattt ctggtacgtc
    61261 accacacgcc gcgatggctt catccaccga cttcacccac gttacgcgat cgtccgtacc
    61321 cggttgactg ctgaggataa tatttttgcg tcctggcaac ggacgaccga ttgattccca
    61381 ggtatggcgg cccataatca cgggtttatt taaggtgttg cgtttaaacc aggcgagatc
    61441 ggcaggcagg ttccacggca tggcgttttc catgccgata acgcgatcta ccgctaacgc
    61501 cgcaatcaga ctgatcattg agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa
    61561 gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg
    61621 cgagtccacg ctctctccct ggactcgccg cattacaatg aaacaaaaac aaacagttag
    61681 ctgtaaagtg tgatttacgt cactctttat taggatgagg gtttcgtttc cggttcatcc
    61741 gccatgttgc cggtatgttt accttcttcg gttccctgcc agccatgtcg ttgaattaat
    61801 gacagatgtt cgcggtcctc ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag
    61861 accgccgcgc gggctttggt gtcgttctca accattgcca tctcttccac catctgaata
    61921 ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt
    61981 tccagtgcca gacgcccggt tttcagcgca ccttcgaagg tttcacgctc cggcttttca
    62041 acgcctgcct gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc
    62101 aaatgcggga aatgttcttt caccatctcc gtcagttgca ggttggtttg cggatcatca
    62161 atagcattaa tcagcacttc cgctttcgcc gctccggcag attccagtaa atccatccgc
    62221 gttgcatcgc cataaaacac tttcatacca aatttacgca gcgtttcgat atggtccgga
    62281 tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtaatctgc
    62341 ccaaaacgac cgaatccggc gataatcacg cgcggctgtt cttcgtcgat ctcatcggct
    62401 tcacgcgctt cctcagtaga agattgctca aggcgattga ggatcaccag cagaatcggc
    62461 gttgctgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc
    62521 atattcgcca tctgcgccgc gccaaatacc acaaaggcaa actcactgcc ctgccctaac
    62581 aacaccgcaa accaacgacg ctgtttattt ggcacttgca acggtcgggc aatcagccac
    62641 agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt
    62701 tcaagcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccaacagc
    62761 aaacctttaa atggttcgat atcgctctcc agcgcatgac ggtattccga gcttgccagc
    62821 agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa
    62881 ccaaagccaa acacgaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg
    62941 gctacaaaac gcagcgccgg acgcgtgaca tagcgcccca gcaataccac cagcaccagc
    63001 gcacccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga ggcactgctc
    63061 gttgccagta gcggaatcat cgccaccagc gggatcgccg cgatatcctg gaacagcagc
    63121 accgcaaagg cactgcgacc catttgcgtc accatcagat tgcgttcatt catcgcctgc
    63181 atggcaatcg ccgtagagga gagcgctagc gtcatgccga tcaattccgc gacctgccag
    63241 cgcagcccaa gtaacatgca gaacagcccc agcaggccgc cgcaaatcac catctgcaat
    63301 gcgccaccgc cgaacactgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg
    63361 ccgataataa acagcatcag caccacccca atctcggcaa agtgcagaat agattcggca
    63421 tcggtcacca gtcgcagccc ccacgggcca ataatgcagc cggcgatcag gtagccaagt
    63481 accgatccca gaccaagacg taccgcaatg ggtacaatca gcgctgccga accgagataa
    63541 atcagcgcct gaatcagcgt atggctatcc atgatgggcc tcctgccatt ccagcagacg
    63601 ttgcttatag tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg
    63661 catggcaaac ggtggcagcc agttcagtcc gcagtagatt gccgtcgcct gtagcggctg
    63721 cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt
    63781 cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccatgac cgtaagccca
    63841 gccgtgggag aaaactttat cgatccaaag tttgaggagc ggaggaatgc tgtaccactg
    63901 catcgggtgc tgccagacga tcagatcggc gcgagacagc gcctcctgct cggcggcaat
    63961 atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt
    64021 ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata
    64081 aattataaga atcataggta gcccgcgtga ttacgccttt gttttcccac aaagtgtagt
    64141 catatattca cgcggcttat agtcaataat attgattcag aaaagagaga aatctgaaca
    64201 atttacccca ttctctgccc gcggatcgtc atattgctgg tttgcgccag tgacatccct
    64261 cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat acccgccccc
    64321 attagcatcg tgttactgat accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa
    64381 acaatggtgc caatacggct taaggacata atcacgccca cggcagaggc gcggatatct
    64441 gtcgggaaga gttcattagg atagagccac tgcaaattac ccggcccgcc agagaaaaag
    64501 gcatacaccg caaaggccat cactaccagc cagatcccca tatccgggat cagccccaaa
    64561 accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg ccgtccggca
    64621 gtgtttaacc acagcatcgg cggaatacag ccgagcataa agaacaggct aatcaccacg
    64681 ttccccagcg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat ttgcgggcca
    64741 aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc aacaaacaga
    64801 acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgctg cggctgctct
    64861 tcatcgaaag ccaccggttc gccaaacagt tttatcatca tctcttcgca ctcttttact
    64921 cgccctttgc gtaataacca gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc
    64981 aacaaacagg ggatcgccgc gctacccagc atccagcgcc agccgccttc cacatcataa
    65041 agccagtagc cgaccagatc ggcgcaggtc gcgccgacat accacatggc ggcgataaag
    65101 ctgatggaaa acgcccgctg acgggtactg gagaactcgg tgatcattga ggtggcgatg
    65161 ggataatctg caccgatgac aatgccgata agtacccgca tcaccaacag ttcgacgggg
    65221 gatgaaacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc aatgaggaac
    65281 attttgcgcc gtccgacttt atcggaaata tagccaaaca gcgatgtgcc aacgaacagc
    65341 ccggcgagcg ttcccgcgcc caacaagcca atccagtcag cgtccagttt tagcgccgga
    65401 gtcagttgct ccagcgccac gccaatcatt accagtacat aaccatccag aaacggacca
    65461 ccgcttcccc acagcaaaat gcggcggtga attgaggaga atttgagatc gtcaaagttt
    65521 ctggacggtt gcatgggtta ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt
    65581 atcaaaccaa catcagccgt aacggaactc cacaccaaag gtgccgcgcg ggtattccca
    65641 ctgttccagc gccgacccca gccccagaat gcgacaggtg ccgcactcca gacatccggc
    65701 gtaatcgaag cgcacactgc cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt
    65761 caccagcagc tccagggcct gtttatcggc atcggccttc acaacaatgt gcggatgctc
    65821 ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc acattgacgg gagaagtcat
    65881 aaaacggtca ctcctttcat gccatccttg atcagattga tgaagcccac tttcttgccg
    65941 tggcggagga ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt gaacaggtca
    66001 cgcgccacgc ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag
    66061 gccggtagtt tctggtacat acgcatatcg cgcagcgggc cactctcaag atgctgacga
    66121 tattccgcga gtttttgctt actgaaatcg tcgcttttca tcgctgaaag cacggttttt
    66181 gctgcagctt ccccggcggc aatcgccaga tccataccgc gaatggtaaa accgaggttc
    66241 atacacattc cggcggcatc accggcaatc aatacgccgt caccaaccaa ctccggcagc
    66301 atgttgatgc ctgcttccgg cactacgtga gcggaatatt ccaccagctt gccgcccgcg
    66361 atcagcggtg ccactgccgg atgctgtttg aaatcttcca gcatttgcgg caccgatttt
    66421 tttgcgtcat gcagatgatg cagaccacaa accagcccca gcgacagggt attttcattg
    66481 gtataaagga agccgccgcc catcaggcca tcggtgggtg atcccgcaaa cagacaagcc
    66541 gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc
    66601 agttccttca cgccaaccgc cacatccgtc ggtttaacgc gttttgccat ccccagcttt
    66661 tcggcaagga tggagttcac tccatcagca aggatcaccg ttttcgcttc aatcacatcg
    66721 ccatcggctt ctacaccgac gactttgcca tcgcgctgta cgaggttatc gacgcggatc
    66781 ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca tcagccaggc atcaaattta
    66841 ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt cgtcaccatt gcagtagtcc
    66901 atagtcatcg ctgacttttc cgtcataaac gcgagttttt catgggtgat caggcgttct
    66961 acgggggcgg agtcggcgaa accaggaata atgtgttcca ggctgtgggc atagagacgc
    67021 ccgccggtga cgttcttggc acctgcggaa ttgccacgct cgataactaa cacttgcgcc
    67081 ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg
    67141 gcgtcaaaga tatcttcgga cataagagct ccctgtcagc ggcagggcca aaatgcagcc
    67201 ctgccagagt ggatcaacgc gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt
    67261 caccaacaat gccgtaatcc gcgtactgga agatcggcgc atttttatct ttattgatgg
    67321 cgaaaatggt ttgcgatgca ttagcaccaa ccatgtgctg gatctgcccg gagatcccca
    67381 ccgccaggta cagttcaggt ttcagcatca ggttggagat accgacatag cgttcgtgct
    67441 ccatccattt ttcgttttcc gccaccggac gagaacaggc caactccgca cctatcgcct
    67501 tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc aataccgcga ccgacgctga
    67561 ccaccagacg ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc
    67621 gggtaatcgc cacagccgga gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg
    67681 cgtctggctg agccgcatcg aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg
    67741 caatgcgttc ttcgccaatc gccagaccac cgtaaaccat gtgtttcact gtcgctttac
    67801 cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc cgctttaagg cgataaccca
    67861 gttttgccgc cagtaatttg ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc
    67921 cgtgctggcg aatagtgtca gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg
    67981 gtttgccgtt taatttccag acatgattag cgccgagctg gattgcctgt acgccgtcgg
    68041 catcattgag gacaaaggtg ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt
    68101 ccggcagacg agaaggggta tcgctgaata cccagacttg agaaaacgtg ttcatagcat
    68161 cccctgtaat taaatgactt tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc
    68221 gtcgccttca atcacgatgc gctgacgttc gcgctgtttc ggcgcagcaa cctgttgttc
    68281 agaccaggcg tccacggcgt taaaaccgat atccgccgcc gaccatacct ggactggctt
    68341 tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt ggggagttga tatcagtgga
    68401 aacagcaaca accgcaggca gcggaatgct taaggtttcg gtttcatctt ccagttcgcg
    68461 ctcaacggtg agggtatctg cagtcaggga gataattttg ctgacgccgt taactgccgg
    68521 aatattgagg atttcgccca ccagcagacc aacctgctgg gcataaaggt cggaagaacc
    68581 atcgccacag aggatcaaat caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc
    68641 cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc accacaatca gttcatccgg
    68701 gccgcgcgat agcacatctt tacgcccttt ggcgttggtc agggctttac cgcccacact
    68761 taaggccgtc acctgcgcct ctgctgcctg ttgctttagc tggcaagccg cttcaatagc
    68821 gttcagatcg tattggctta ttttggcatc ggctttgctg aagtctaatg aaccatcagc
    68881 attattgacc gcaatatcct gttcatcagg cacgcactta tagcaagtaa taatcttcat
    68941 tgcatctcca gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa ttacagatat
    69001 cacgcccttt aatttggaaa taaaacttta atcaccaata ttgaaaatgt cacacgcaga
    69061 taaatccaac tttcaatatt gttaagttcc tcaccaatat tgaaaacatc gcgtagcaaa
    69121 aagaaatttt caatattgct ttatggatca ccaatattga aagtcagatg ctattttttt
    69181 actcacaaca caacataaca aactgattaa taatcaaaat taacgagaaa agcgccctct
    69241 attcacccga aacaaaaatg tgataacaat cacagaatgc agcttattga atacccatta
    69301 tgagttagcc attaacgcgt ccacgaggtt aataataatt atattaaatg ttaacaaaaa
    69361 taaaacaaac gggaacgcaa aataaatatt cgttttcaca gtggaattaa ctcatgaaga
    69421 atgaaaagag aaaaacggga atagaaccga aggttttctt tccgccgtta ataatcgtcg
    69481 gcatactttg ttggcttaca gtcagagatc tggatgcagc gaatgtcgtt attaatgctg
    69541 tattcagtta cgtcaccaat gtatggggat gggcatttga atggtatatg gtggtgatgc
    69601 ttttcggttg gttctggctg gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac
    69661 cacctgaatt tagtaccgcc agttggatct ttatgatgtt cgcctcctgt acgtctgctg
    69721 ccgtactgtt ctggggatcg attgagatct actactacat ctccaccccg ccgtttggct
    69781 tagaaccgaa ctcgacaggg gcgaaagagt tggggctggc ttacagcttg ttccactggg
    69841 gacctctgcc gtgggccact tacagcttcc tttcagtcgc cttcgcttac ttcttctttg
    69901 tccgcaaaat ggaagtgatt cgccccagct cgacactggt gccgctggta ggtgaaaaac
    69961 acgccaaagg gttgttcggc actatcgtcg acaacttcta tctcgtcgcc ttgatcttcg
    70021 cgatgggtac cagtctgggc cttgccacgc cgctggtgac cgagtgtatg caatggttgt
    70081 ttggcattcc gcataccctg caactggacg ctatcatcat tacctgctgg attatcctca
    70141 acgccatttg cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta
    70201 gttacctgag cttcctgatg ctgggttggg tgttcattgt cagcggtgcc agcttcatca
    70261 tgaactactt caccgattcg gtggggatgt tgctgatgta tctgccgcgc atgttgttct
    70321 ataccgatcc catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc tactgggcat
    70381 ggtgggtgat ttatgctatc cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta
    70441 ctgtgcgtga actgtgcttc ggcatggtgc tggggctgac agcgtcaacc tggatcctgt
    70501 ggactgtact cggtagtaac actctgctgt tgatagataa aaacatcatc aacattccaa
    70561 atctgatcga acagtacggt gtggcgcgcg ccatcattga aacctgggcc gctctgccac
    70621 tcagcaccgc caccatgtgg ggcttcttca tcctctgctt tattgccacc gttacgctgg
    70681 ttaacgcctg ctcttatacc ctggcgatgt ccacttgccg cgaagtacgc gatggtgaag
    70741 aaccacctct gctggtgcgt atcggttggt caattctggt tggcattatc ggtattgttc
    70801 tgctggcgct cggcggcctg aaaccgattc aaaccgccat tatcgccgga ggatgcccgc
    70861 tgttcttcgt caacattatg gtgacgctct cctttattaa agacgcgaaa cagaactgga
    70921 aagattaatt aacccccaaa atatcaagag gttgaaagat ggattttaat ttaaatgatg
    70981 agcaggaact gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac tgggaggcct
    71041 attttgccga gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa gcactggcgg
    71101 atatgggtat cgacagtctg ctgatccctg aagagcacgg tggtctggac gcggggtttg
    71161 ttactctcgc cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc tatgtgctgt
    71221 accagttgcc gggcgggttc aacaccttcc tgcgcgaagg cacacaagag cagatcgaca
    71281 agattatggc tttccgcggc accggtaagc agatgtggaa ctcagcgatt accgaaccgg
    71341 gcgcgggctc cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat ggtaagattt
    71401 atcttaatgg tagtaagtgt tttattacca gcagcgccta caccccgtac atcgtggtga
    71461 tggcgcgcga cggggcttct ccggacaaac ctgtctacac cgaatggttt gttgatatga
    71521 gcaaaccggg catcaaagtg accaaacttg aaaagctcgg tctgcgtatg gatagctgct
    71581 gtgaaatcac ctttgacgac gtggaactgg acgagaaaga catgttcggt cgggaaggta
    71641 acggctttaa ccgcgtcaaa gaagagttcg accatgaacg tttcctggta gccctcacca
    71701 actacggtac ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaat cagcgcgtgc
    71761 agtttggcga ggctattggt cgtttccagt tgattcagga aaaattcgcc cacatggcga
    71821 tcaaattaaa ctccatgaaa aacatgctgt atgaagcagc gtggaaagca gacaacggca
    71881 ccatcacctc tggcgatgca gcgatgtgca aatacttctg cgccaatgcg gcatttgaag
    71941 ttgtggatag cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca
    72001 gccgcttctg gcgtgatctg cgtgtagacc gcgtctccgg gggatctgac gaaatgcaga
    72061 tcctgacgct gggtcgcgcg gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg
    72121 cgacgctaat gcgtcttatc aggcctacgg gagcacgaat gtaggccgga taaagcgttt
    72181 acgccgcatc cggcagtcat gcgctacata aatttttcag gagctaataa tggatcatct
    72241 acccatgccg aaattcgggc cgttggccgg attgcgcgtt gtcttctccg gtatcgaaat
    72301 cgccgggccg ttcgccgggc aaatgttcgc agaatggggt gcggaagtta tctggatcga
    72361 gaacgtcgcc tgggccgaca ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg
    72421 caatttgcac gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag cgtttctgaa
    72481 attaatggaa accaccgata tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg
    72541 tggcattacc gatgaagtac tgtggcagca caacccgaaa ctggttatcg ctcacctgtc
    72601 cggttttggt cagtacggca ccgaggagta caccaatctt ccggcctata acaccatcgc
    72661 ccaggccttc agtggttacc tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt
    72721 cccgtatacc gccgattact tttctggcct gaccgccacc acggcggcgc tggcagcact
    72781 gcataaagtg cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt atgaagtgat
    72841 gctgcgtatg ggccagtact tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg
    72901 catgagcaaa ggtaaagatc cctactacgc cggttgcggt ctgtataaat gcgccgacgg
    72961 ctacatcgtg atggaactgg tgggcattac ccaaattgaa gagtgcttta aagatattgg
    73021 cctcgcacat ctgctcggta cgccggaaat cccggaaggc actcagctta tccaccgtat
    73081 cgaatgccct tacggcccac tggtagaaga gaaactcgat gcctggctgg cggcacatac
    73141 catcgcagaa gttaaagaac gctttgccga actgaatatc gcctgtgcca aagtactgac
    73201 cgtaccggaa ctggaaagca atccacagta tgttgcccgt gaatcaatca ctcagtggca
    73261 aacgatggat ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca aaaataaccc
    73321 cggacaaatc tggcgcggaa tgccctcaca tggcatggac acggctgcca ttttgaaaaa
    73381 tatcggctac agcgaaaacg acattcagga gttggtcagc aaaggtctgg ccaaagttga
    73441 ggactaaacg cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa
    73501 caaaaatgga tagaggtgca atggatatca ttggcggaca acatctacgt caaatgtggg
    73561 acgatctggc ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag
    73621 tcgttaaccg gtatagttat cttgagttaa atcaggagat taaccgcacg gcaaacctgt
    73681 tttatacgct ggggattcgc aaaggcgaca aggttgcact acatctcgac aactgcccgg
    73741 aatttatctt ttgctggttc gggctggcaa aaattggcgc gattatggtg ccgattaacg
    73801 cccgcctgtt acgcgaggaa agcgcgtgga tcctgcaaaa tagccaggcg tgcctgctgg
    73861 tgaccagtgc gcaattctat cctatgtatc aacagattca gcaggaagat gccactcaat
    73921 tgcggcacat ttgcctgaca gatgtggcac ttcccgctga tgatggcgtg agttcgttta
    73981 ctcaactgaa aaatcaacaa cctgccacct tgtgctatgc accgccgcta ttgactgacg
    74041 atacggcgga aattctcttc acctccggca ccacctcccg accgaaaggt gtggtgatta
    74101 cccattacaa cctgcgcttc gctggatatt actccgcctg gcagtgtgca ctgcgtgacg
    74161 atgacgtcta cctgacggta atgcctgcgt ttcatatcga ttgccagtgt actgcggcga
    74221 tggcggcgtt ttctgccggg gccacctttg tgctggtcga gaaatacagc gcccgcgcct
    74281 tctggggaca ggtgcagaag taccgcgcca ccattaccga atgtattccg atgatgatcc
    74341 gtacgttgat ggtgcagccg ccttcagcga acgatcggca acaccgcctg cgggaagtga
    74401 tgttttatct caacttgtcg gagcaggaaa aagatgcgtt ttgtgaacgc ttcggcgttc
    74461 gcttgctgac gtcttatggg atgacggaaa ccattgtggg cattatcggc gatcgccctg
    74521 gcgataaacg acgctggccg tcgattggtc gggcggggtt ttgctacgaa gcggagatcc
    74581 gcgacgatca caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt attaaaggcg
    74641 taccagggaa aaccatcttc aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc
    74701 tggaagccga cggctggcta cataccggcg ataccggata ctgcgacgaa gagggctttt
    74761 tttatttcgt cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg
    74821 tggagctgga aaatatcatc gccacccatc caaaaattca ggatatcgtg gttgtgggta
    74881 ttaaagattc gattcgcgat gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa
    74941 cattgagcga agaggaattt ttccgcttct gcgaacaaaa tatggcgaaa tttaaagtgc
    75001 cctcttatct ggagatcaga aaagatctgc cacgtaattg ctcggggaaa ataattagaa
    75061 agaatctgaa ataaatgaaa cagcagggaa ctaccctgcc agctaataat cacgctataa
    75121 aacaatatgc attttttgca gggatgttgt cgtccctgaa aaagcaaaaa tggagaaaag
    75181 gaatgagtga atcattacat ctgacccgca atggatcaat tctggaaatt acccttgatc
    75241 gtccaaaagc gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa
    75301 atttccgtga cgatccgcaa ttacgtgtcg ccattattac cggtgccgga gagaagttct
    75361 tttccgcggg ctgggattta aaagcggcag cagaaggcga agcaccggat gctgactttg
    75421 gcccgggtgg atttgcggga ttaaccgaaa ttttcaatct cgacaaaccg gttatcgcag
    75481 ctgtgaacgg ctatgccttt ggcggcggtt tcgaactggc gctggcggca gattttattg
    75541 tttgtgccga taacgccagc ttcgccctgc cggaagccaa gctgggcatc gttcctgaca
    75601 gcggcggcgt gctgcgtcta ccgaagatcc tgccgcctgc catcgtcaat gaaatggtga
    75661 tgaccggcag acgaatgggc gcagaagagg cgctgcgttg ggggatagtc aaccgcgtgg
    75721 ttagccaggc ggaactgatg gataacgccc gcgaactggc gcagcagctg gttaacagcg
    75781 ccccgctggc gattgcggcg ctgaaagaga tcttccgcac caccagcgaa atgccggtag
    75841 aagaagcgta tcgctatatt cgcagcggcg tgttgaaaca ctatccgtcg gttctgcatt
    75901 cggaggatgc cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg gtgtggaaag
    75961 gacgttaaca accgtgagct attacgcctt tgaggggtta attccggtgg ttcacccgac
    76021 ggcgtttgtc catcccagtg ccgtcttgat tggcgatgtg attgtgggag ccggtgtcta
    76081 catcggccca ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc
    76141 caatattcag gatggctgca ttatgcatgg ctactgcgac actgacacca tcgttgggga
    76201 aaacggccat atcgggcacg gagcgatcct gcatggttgt gtgattggtc gcgatgcatt
    76261 ggtcgggatg aacagcgtga ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc
    76321 cgccatgagc tttgtcaaag caggctttca cggcgagaaa cgccagttgt tgatgggtac
    76381 gccagcccgc gctgtacgca gtgttagtga cgacgagtta cactggaaac ggttgaatac
    76441 caaagagtat caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa cacagccact
    76501 gaggcaaatg gaggaaaatc gcccccgttt gcaggggacg acggatgtga cgcctaaacg
    76561 gtgatcttgc cggatgcgac ctggttgctg gataagatgc tacaacatcg catctggcaa
    76621 gcaggacgcc cggtatcaac gagataaaat taacgacgca tactctttga caacatcatc
    76681 tgccacttct gctcgcggtt aagttccgtg agcggaacgg caggaatacg cggcgtcttt
    76741 cttacgccga ctaatttatc ccggctgtta ttgcgcaggc gcgcgtaaat ttgctcatcg
    76801 atatcgacca cttttaccag tcgctggcac tggcatcccc gtccttccag cttattaggg
    76861 atcatcttaa cttcgcagct tatttctgcg acttccgaca gaatataagt cagggtatta
    76921 accgccttgc tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga
    76981 ttttcagcca tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca
    77041 cacatacgaa ctccagtttc acaaattaaa agtctggcta tgactcgttt tgaagcaatt
    77101 aaacaaggcc atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag
    77161 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
    77221 gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcac attagcggat
    77281 gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc tgcaccgaaa
    77341 ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
    77401 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
    77461 tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc ttgatgcggt
    77521 cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta atgcccgctt
    77581 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
    77641 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc aacgcacgac
    77701 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg
    77761 tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac agcgggtcaa
    77821 cacccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa
    77881 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
    77941 ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt
    78001 taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc
    78061 gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
    78121 gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg cccgcctgct
    78181 cgatgtgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt
    78241 caacttctac cgcgtcatca aggaagtgat ccagcaacac tggcgcatcg ttagacacgc
    78301 tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
    78361 gaccgccgag aacgtaagac ggacgtacca ccagcgggta gccaatctct ttcgccttct
    78421 ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct
    78481 caaccgcatg ctggaagcgt tcacggtctt ctgcacggtc gatagcatcc gggctggtgc
    78541 cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc
    78601 cgtactgaac gataacgcct ttcggcttct caatacgcac gatttctagc acatcttcca
    78661 gtgttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtggag acggtttccg
    78721 ggttacagtt aaccataatg gtttcgtaac cgtcttcgcg cagtgccagc gaggcgtgta
    78781 cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg ccgagaacca
    78841 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
    78901 tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta taaaccgggt
    78961 gcaggtcata ctggtcgcgc agcttgcgga tttccgcttc gcgcacgccc gccagttttg
    79021 ccagacgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc
    79081 cagtgatgcc cacttccgct actttctctt ccagacgcac cagttcttca atctgtacca
    79141 ggaaccagcg gtcaatattg gtcaggttga acacgccgtc cacagacagg cccgcacgga
    79201 aggcatcggc gatgtaccag atacgctctg cgccagcatc tttcagttcg cgacggattt
    79261 tggttaacgc ttccgggtca tcgaggctca ctttcgggtc gaatccagtc gcaccgactt
    79321 ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt
    79381 cgccaaccga tttcatctga gtggtcagac ggtcgttagc accagcgaat ttttcgaagt
    79441 tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac
    79501 gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc agtttcgccg
    79561 ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc
    79621 gcgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat
    79681 tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga
    79741 tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtat
    79801 ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat
    79861 cacgcaccac ttccatctcg tactctttcc agccaatcag cgactcatca atcagcaact
    79921 ctttggtcgg agagagatcc agaccgcggg cgcaaatttc ttcaaactct tcgcggttat
    79981 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
    80041 ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg
    80101 tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa
    80161 tcgcatcggc ggtcgcacca atcatagtca cgccgaactc ttccaacacg ccctgacgct
    80221 ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc accgcgtccg
    80281 ggcgctcttt ttcaataatc ttgcgtacca cttcccagtg aatcggctcg atgtaggtcg
    80341 catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgaccc
    80401 ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg
    80461 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
    80521 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactc
    80581 gataaagtgg tcgaacaacg gcgcggcgtc gtgtggacca gggctggctt cagggtgccc
    80641 ctggaagctg aacgccggtt tatcggtgcg atgaatgccc tgtaacgtac cgtcgaacag
    80701 ggatttatgc gtgacacgca ggtttgctgg taatgttgct tcgtccaccg caaaaccgtg
    80761 gttctgggcg gtgatcatca ccacgttttt ctcaacatct ttaaccggat ggttgccgcc
    80821 gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg
    80881 accgaggcag atgccgaaga ccggaatatc ggtttcgagg aatttctgga tggcggtaat
    80941 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatac cgtctggatt
    81001 cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct
    81061 gtccaccaac atgcgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa
    81121 cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
    81181 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cattcagacc
    81241 tgggaacgcg cgggcttttt ctaacgccaa cgccgcatcc gggttatcgc ctgcgataat
    81301 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
    81361 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
    81421 gttgctggca atcagcggca ggtcgcgaat caccagacct tgtgcatgta cctgagaaga
    81481 ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat
    81541 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
    81601 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
    81661 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac
    81721 tttatttgca tattaattca
//
LOCUS       DAZKGS010000005        68904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKGS010000005 DAZKGS010000000
VERSION     DAZKGS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..793
                     /gene="ykgE"
                     /locus_tag="ACHOQY_000349"
     CDS             74..793
                     /gene="ykgE"
                     /locus_tag="ACHOQY_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9676511.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            804..2231
                     /gene="ykgF"
                     /locus_tag="ACHOQY_000350"
     CDS             804..2231
                     /gene="ykgF"
                     /locus_tag="ACHOQY_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9676512.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2224..2919
                     /gene="ykgG"
                     /locus_tag="ACHOQY_000351"
     CDS             2224..2919
                     /gene="ykgG"
                     /locus_tag="ACHOQY_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9676513.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(3165..3830)
                     /gene="ykgH"
                     /locus_tag="ACHOQY_000352"
     CDS             complement(3165..3830)
                     /gene="ykgH"
                     /locus_tag="ACHOQY_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP9676514.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVILFIIAICMAMMLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(4043..5713)
                     /gene="betA"
                     /locus_tag="ACHOQY_000353"
     CDS             complement(4043..5713)
                     /gene="betA"
                     /locus_tag="ACHOQY_000353"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9676515.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(5727..7199)
                     /gene="betB"
                     /locus_tag="ACHOQY_000354"
     CDS             complement(5727..7199)
                     /gene="betB"
                     /locus_tag="ACHOQY_000354"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9676516.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(7213..7800)
                     /gene="betI"
                     /locus_tag="ACHOQY_000355"
     CDS             complement(7213..7800)
                     /gene="betI"
                     /locus_tag="ACHOQY_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9676517.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            7929..9962
                     /gene="betT"
                     /locus_tag="ACHOQY_000356"
     CDS             7929..9962
                     /gene="betT"
                     /locus_tag="ACHOQY_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9676518.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            10535..14518
                     /gene="ehaA"
                     /locus_tag="ACHOQY_000357"
     CDS             10535..14518
                     /gene="ehaA"
                     /locus_tag="ACHOQY_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP9676519.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            14660..15748
                     /gene="pdeL"
                     /locus_tag="ACHOQY_000358"
     CDS             14660..15748
                     /gene="pdeL"
                     /locus_tag="ACHOQY_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9676520.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(15790..16722)
                     /gene="yahB"
                     /locus_tag="ACHOQY_000359"
     CDS             complement(15790..16722)
                     /gene="yahB"
                     /locus_tag="ACHOQY_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9676521.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(16814..17311)
                     /gene="yahC"
                     /locus_tag="ACHOQY_000360"
     CDS             complement(16814..17311)
                     /gene="yahC"
                     /locus_tag="ACHOQY_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9676522.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            17569..18174
                     /gene="yahD"
                     /locus_tag="ACHOQY_000361"
     CDS             17569..18174
                     /gene="yahD"
                     /locus_tag="ACHOQY_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9676523.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            18214..19077
                     /gene="yahE"
                     /locus_tag="ACHOQY_000362"
     CDS             18214..19077
                     /gene="yahE"
                     /locus_tag="ACHOQY_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9676524.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            19067..20614
                     /gene="fdrA"
                     /locus_tag="ACHOQY_000363"
     CDS             19067..20614
                     /gene="fdrA"
                     /locus_tag="ACHOQY_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9676525.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            20614..22032
                     /gene="yahG"
                     /locus_tag="ACHOQY_000364"
     CDS             20614..22032
                     /gene="yahG"
                     /locus_tag="ACHOQY_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9676526.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            22175..23125
                     /gene="yahI"
                     /locus_tag="ACHOQY_000365"
     CDS             22175..23125
                     /gene="yahI"
                     /locus_tag="ACHOQY_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9676527.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            23135..24517
                     /gene="yahJ"
                     /locus_tag="ACHOQY_000366"
     CDS             23135..24517
                     /gene="yahJ"
                     /locus_tag="ACHOQY_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9676528.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            24816..25226
                     /locus_tag="ACHOQY_000367"
     CDS             24816..25226
                     /locus_tag="ACHOQY_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9676529.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            25480..26463
                     /locus_tag="ACHOQY_000368"
     CDS             25480..26463
                     /locus_tag="ACHOQY_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9676530.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            26512..27996
                     /locus_tag="ACHOQY_000369"
     CDS             26512..27996
                     /locus_tag="ACHOQY_000369"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9676531.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            27989..28960
                     /locus_tag="ACHOQY_000370"
     CDS             27989..28960
                     /locus_tag="ACHOQY_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9676532.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            28957..29913
                     /locus_tag="ACHOQY_000371"
     CDS             28957..29913
                     /locus_tag="ACHOQY_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9676533.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            30000..31049
                     /gene="yahK"
                     /locus_tag="ACHOQY_000372"
     CDS             30000..31049
                     /gene="yahK"
                     /locus_tag="ACHOQY_000372"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9676534.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            31292..32107
                     /gene="yahL"
                     /locus_tag="ACHOQY_000373"
     CDS             31292..32107
                     /gene="yahL"
                     /locus_tag="ACHOQY_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP9676535.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            32519..32764
                     /gene="yahM"
                     /locus_tag="ACHOQY_000374"
     CDS             32519..32764
                     /gene="yahM"
                     /locus_tag="ACHOQY_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP9676536.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(32781..33452)
                     /gene="yahN"
                     /locus_tag="ACHOQY_000375"
     CDS             complement(32781..33452)
                     /gene="yahN"
                     /locus_tag="ACHOQY_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9676537.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            33599..33874
                     /gene="yahO"
                     /locus_tag="ACHOQY_000376"
     CDS             33599..33874
                     /gene="yahO"
                     /locus_tag="ACHOQY_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9676538.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(33972..35558)
                     /gene="prpR"
                     /locus_tag="ACHOQY_000377"
     CDS             complement(33972..35558)
                     /gene="prpR"
                     /locus_tag="ACHOQY_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9676539.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            35797..36687
                     /gene="prpB"
                     /locus_tag="ACHOQY_000378"
     CDS             35797..36687
                     /gene="prpB"
                     /locus_tag="ACHOQY_000378"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9676540.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            36732..37901
                     /gene="prpC"
                     /locus_tag="ACHOQY_000379"
     CDS             36732..37901
                     /gene="prpC"
                     /locus_tag="ACHOQY_000379"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9676541.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            37935..39386
                     /gene="prpD"
                     /locus_tag="ACHOQY_000380"
     CDS             37935..39386
                     /gene="prpD"
                     /locus_tag="ACHOQY_000380"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9676542.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            39426..41312
                     /gene="prpE"
                     /locus_tag="ACHOQY_000381"
     CDS             39426..41312
                     /gene="prpE"
                     /locus_tag="ACHOQY_000381"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9676543.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            41651..42910
                     /gene="codB"
                     /locus_tag="ACHOQY_000382"
     CDS             41651..42910
                     /gene="codB"
                     /locus_tag="ACHOQY_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9676544.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            42900..44183
                     /gene="codA"
                     /locus_tag="ACHOQY_000383"
     CDS             42900..44183
                     /gene="codA"
                     /locus_tag="ACHOQY_000383"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9676545.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(44223..45122)
                     /gene="cynR"
                     /locus_tag="ACHOQY_000384"
     CDS             complement(44223..45122)
                     /gene="cynR"
                     /locus_tag="ACHOQY_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP9676546.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            45232..45891
                     /gene="cynT"
                     /locus_tag="ACHOQY_000385"
     CDS             45232..45891
                     /gene="cynT"
                     /locus_tag="ACHOQY_000385"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP9676547.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            45922..46392
                     /gene="cynS"
                     /locus_tag="ACHOQY_000386"
     CDS             45922..46392
                     /gene="cynS"
                     /locus_tag="ACHOQY_000386"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP9676548.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            46398..47579
                     /gene="cynX"
                     /locus_tag="ACHOQY_000387"
     CDS             46398..47579
                     /gene="cynX"
                     /locus_tag="ACHOQY_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP9676549.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(47658..48911)
                     /gene="lacY"
                     /locus_tag="ACHOQY_000388"
     CDS             complement(47658..48911)
                     /gene="lacY"
                     /locus_tag="ACHOQY_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP9676550.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(48963..52037)
                     /gene="lacZ"
                     /locus_tag="ACHOQY_000389"
     CDS             complement(48963..52037)
                     /gene="lacZ"
                     /locus_tag="ACHOQY_000389"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9676551.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(52160..53242)
                     /gene="lacI"
                     /locus_tag="ACHOQY_000390"
     CDS             complement(52160..53242)
                     /gene="lacI"
                     /locus_tag="ACHOQY_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9676552.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(53319..54152)
                     /gene="mhpR"
                     /locus_tag="ACHOQY_000391"
     CDS             complement(53319..54152)
                     /gene="mhpR"
                     /locus_tag="ACHOQY_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP9676553.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            54343..56007
                     /gene="mhpA"
                     /locus_tag="ACHOQY_000392"
     CDS             54343..56007
                     /gene="mhpA"
                     /locus_tag="ACHOQY_000392"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP9676554.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            56009..56953
                     /gene="mhpB"
                     /locus_tag="ACHOQY_000393"
     CDS             56009..56953
                     /gene="mhpB"
                     /locus_tag="ACHOQY_000393"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP9676555.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            56971..57837
                     /gene="mhpC"
                     /locus_tag="ACHOQY_000394"
     CDS             56971..57837
                     /gene="mhpC"
                     /locus_tag="ACHOQY_000394"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP9676556.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            57847..58656
                     /gene="mhpD"
                     /locus_tag="ACHOQY_000395"
     CDS             57847..58656
                     /gene="mhpD"
                     /locus_tag="ACHOQY_000395"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP9676557.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            58653..59603
                     /gene="mhpF"
                     /locus_tag="ACHOQY_000396"
     CDS             58653..59603
                     /gene="mhpF"
                     /locus_tag="ACHOQY_000396"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP9676558.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            59600..60613
                     /gene="mhpE"
                     /locus_tag="ACHOQY_000397"
     CDS             59600..60613
                     /gene="mhpE"
                     /locus_tag="ACHOQY_000397"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP9676559.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            60888..62099
                     /gene="mhpT"
                     /locus_tag="ACHOQY_000398"
     CDS             60888..62099
                     /gene="mhpT"
                     /locus_tag="ACHOQY_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP9676560.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            62201..62740
                     /gene="yaiL"
                     /locus_tag="ACHOQY_000399"
     CDS             62201..62740
                     /gene="yaiL"
                     /locus_tag="ACHOQY_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9676561.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(62865..63698)
                     /gene="fghA"
                     /locus_tag="ACHOQY_000400"
     CDS             complement(62865..63698)
                     /gene="fghA"
                     /locus_tag="ACHOQY_000400"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9676562.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(63791..64900)
                     /gene="frmA"
                     /locus_tag="ACHOQY_000401"
     CDS             complement(63791..64900)
                     /gene="frmA"
                     /locus_tag="ACHOQY_000401"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9676563.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(64935..65210)
                     /gene="frmR"
                     /locus_tag="ACHOQY_000402"
     CDS             complement(64935..65210)
                     /gene="frmR"
                     /locus_tag="ACHOQY_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9676564.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(65396..66169)
                     /gene="yaiO"
                     /locus_tag="ACHOQY_000403"
     CDS             complement(65396..66169)
                     /gene="yaiO"
                     /locus_tag="ACHOQY_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP9676565.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(66171..66752)
                     /locus_tag="ACHOQY_000404"
     CDS             complement(66171..66752)
                     /locus_tag="ACHOQY_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9676566.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            complement(66730..67926)
                     /gene="yaiP"
                     /locus_tag="ACHOQY_000405"
     CDS             complement(66730..67926)
                     /gene="yaiP"
                     /locus_tag="ACHOQY_000405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9676567.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(67936..68607)
                     /locus_tag="ACHOQY_000406"
     CDS             complement(67936..68607)
                     /locus_tag="ACHOQY_000406"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP9676568.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
BASE COUNT        16498 a        17147 c        18679 g        16580 t
ORIGIN      
        1 ttattgatga ttgatccccc ggtggggggg aaacatcggg cataaatggg catgaagtaa
       61 tggagtatta gttgtgaatg tcaatttctt tgtcacctgt attggtgacg ccctgaaatc
      121 aagaatggca cgagactccg tgctgctact ggaaaaactc ggctgtcgcg taaatttccc
      181 ggagaaacag ggatgctgcg gtcagcctgc gatcaatagc ggttatatca aagaagcgat
      241 tccagggatg aaaaatctga tcgccgcact ggaggataac gacgatccca ttatttcacc
      301 ggctggctct tgcacctatg ccgtaaaaag ttacccgacg tatctggcgg atgaacctga
      361 atgggcatca cgtgccgaaa aggttgccgc gcgtatgcag gatctcacct cttttattgt
      421 taataaatta ggggtagtcg atgtaggtgc cagtttgcaa gggagagctg tgtatcaccc
      481 atcttgtagc ctggcccgta agctgggagt gaaggacgag ccacttacgc tgctgaaaaa
      541 tgtgcgtgga ctggagctgt tgacctttgc tgaacaggat acctgctgcg gatttggcgg
      601 cacgttctcg gtcaaaatgg ccgaaatatc cggcgagatg gtgaaagaaa aggttgtgca
      661 cctgatggaa gtccgccctg agtatttaat tggtgctgac gtgagttgcc tgctgaacat
      721 cagtgggcga ttacaacggg aagggcagaa agtcaaagtg atgcatattg ctgaagtgtt
      781 gatgagccgc tgaggatata aagatgtcga tcaaaaccag taatacagat tttaagacac
      841 gcatccgtca gcaaattgaa gatccgatca tgcgcaaagc ggtggcaaac gcgcagcagc
      901 gtattggggc aaatcggcaa aaaatggtcg atgaattggg gcactgggag gagtggcgcg
      961 atcgggccgc ccagatacgt gatcatgttc taagtaatct cgacgcttat ctgtaccagc
     1021 tctcagaaaa agtgacgcaa aacggcggtc acgtctattt tgcaaaaact aaagaagacg
     1081 ccactcgcta cattttacag gttgcccaac gcaaaaatgc ccggaaggtg gtgaaatcta
     1141 aatcgatggt gaccgaagag attggtgtca atcatgtgtt gcaggatgct ggcattcagg
     1201 tgattgaaac cgatctgggt gaatacattc tccagctgga tcaagatccg ccatctcatg
     1261 ttgtggtccc ggcaattcat aaagatcgcc atcagatccg tcgagtgcta cacgaacgtc
     1321 tgggctatga ggggccggaa acgcctgaag cgatgacctt attcatccgg caaaaaatcc
     1381 gcgaagattt cctcagtgct gaaataggta ttaccggctg taatttcgcg gtggcagaga
     1441 caggttcggt atgcctggtg accaatgaag gtaatgcgcg aatgtgtacc acgctgccta
     1501 aaacgcatat tgcagtgatg ggaatggagc gtattgcccc cacgtttgcc gaggtagatg
     1561 tattgatcac catgctggcg cgcagtgccg ttggtgcacg tttgacggga tacaacacct
     1621 ggctgacagg accgcgcgaa gcggggcacg ttgatggtcc tgaagagttt catctggtta
     1681 ttgtcgataa cgggcgttct gaggtgctgg cctctgaatt tcgggatgtg ctgcgctgta
     1741 ttcgctgcgg ggcttgtatg aatacttgtc cggcatatcg ccatattggc ggtcatggat
     1801 atggctctat ttatccaggg ccaattggtg cggtgatttc tccgctactt ggcggctata
     1861 aagattttaa agatttaccc tacgcctgct ctttatgcac tgcttgtgac agcgtgtgtc
     1921 cggtgcgtat tccgctgtca aaactgattt tgcgtcatcg tcgggtgatg gctgaaaaag
     1981 gtatcaccgc aaaagcagag caacgggcga taaaaatgtt cgcttatgcc aatagtcatc
     2041 caggattgtg gaaagtcggg atgatggccg gcgctcatgc ggcaagctgg tttatcaatg
     2101 gcggcaaaac accactcaaa tttggcgcga ttagcgactg gatggaagca cgcgatcttc
     2161 ctgaagctga cggagagagt ttccgtagtt ggtttaagaa acatcaggcg caggagaaaa
     2221 agaatggata atcgaggcga atttttgaat aacgttgctc aggcactggg tcgcccgctg
     2281 cgacttgaac cgcaagcaga agatgcgccg cttaacaact atgctaacga gcggcttacc
     2341 caacttaacc aacagcagcg ctgtgacgcg tttattcagt ttgccagcga tgttatgttg
     2401 acgcgctgtg agctgaccag cgaggcgaag gcggcagaag ctgcaatacg tctgtgtaaa
     2461 gagctgggag atcagtcggt cgtgattagc ggtgacacga ggctggagga attggggatt
     2521 agtgaacgtt tgcagcagga atgcaatgcc gttgtttggg atccggcgaa aggtgccgag
     2581 aatatctcgc aggccgagca ggctaaagtg ggtgtcgtgt atgctgaata tggtttaacc
     2641 gaatcgggag gcgtggttct tttttccgcc gccgggcgcg ggcgttcatt gagcctgctc
     2701 ccggaatctt ctctttttat cctgcgtaaa agcactatct tgccgcgtgt agcgcaactc
     2761 gcagaaaaat tgcatcagaa agcgcaggcc ggtgaacgaa tgccgtcctg cattaacatc
     2821 attagcggcc ctagttcaac ggcggatatt gagcttatca aagtcgtcgg ggttcatggt
     2881 ccggtgaaag cggtgtatct gattattgag gattgctgag agagatatga gatctgaaca
     2941 taaacctgaa gaaggcatac gaggttgaaa atagcgttaa ggacaacctg acttaacccc
     3001 gttccgtcat ggttatgctg ctcttctgta aatatcctgg cattagtaat gccaggatat
     3061 ttacaatgaa aaagacaggc atccattcct gaaaacgggt tcccttcccg gtcattgtca
     3121 cttcagcttt atacaggcac tctctatcag aatgctgttt attactaccc agagttttgt
     3181 tggtattcat ctaccatttt tttgacaagg caaaacatta cagaaattac aatgcttaga
     3241 aaaaatccca gagccaggca aatggcagca gggtatttat tttttatttc tggtgagctt
     3301 atagttacac agccatcacc gttcggatac aatgaccagt tcctggtcag gtatatcatt
     3361 tttgtataaa gctcactgtt aattgctgaa ggtgtttgta tctcatcgag aacttgcctg
     3421 ccattatggt attctgacgc ataccatttg ataatagacg cctgcacgcg agatatttcc
     3481 gcttcagcag ttggtaaatc tctcttatct cctgaccatt ccagctttat ttctctgttc
     3541 ttgacatctg ggctggtgtg aaaaaatgta gtaattcgct gtataacatc ttcagtcatt
     3601 gtggtctgac aggcaaagtt attattgcta tcacagttaa caagtatgcc tggtgttgtc
     3661 tggtttattt ttaggctgta actcactttt atattatctt tattgatata cagaaataaa
     3721 agcatcattg ccatacagat agctataata aaaaggataa cacgaacctt tttcttcaga
     3781 taaaataaaa tctcaatcag atcgatatcc tgtttgattt gttcacgcat aatatatcca
     3841 gaaaataaaa tctgtcgcag ataaggttgt attaatagtc tgtatcagga atgttcgggt
     3901 taaatatcag caaaaagcca gcatcatgga tactggatat gaagcatgag agttacctcg
     3961 gtgtttatat aaggattcgg tccccctctc tggaacggta actctcaatc tgatcggttc
     4021 ctgcgttagt tcacatcacg actcattttt tcgctctcac cggcatccca tttgccacaa
     4081 aatatcccgc cgtgctcctc ggcagcgctt cctgtccacg aatcatatcc gctattttct
     4141 cgccaatcat aattgtcgtg gcgttcaaat tcccggtgat aatctgcggc ataatcgacg
     4201 catccaccac acgcaggcct tctaacccgt gtacgcggcc ttcgccgtca accacggaca
     4261 tctcgtcgta acccattttg caggtaccgc acggatggaa ggcggtttcg gcgtggttac
     4321 gcacgaactc atcgagctgt tcatccgtct gacattccgt gccggggctg atttcgcggc
     4381 cacgatactg atccagcgcg ggttgatgca tgatctcgcg ggtgatgcga attgcgtcgc
     4441 ggaactcctg ccagtcctgc tcgtgcgaca tgtagttaaa cagaatcgcc ggatgctggt
     4501 gcgggtcgcg ggatttaatc cgcacatgcc cacggcttgg cgagcgcatt gagccgacgt
     4561 ggcactggaa accgtgctct ttcactgcat tcgagccgtt atagttaatc gctactggca
     4621 ggaaatggta ctgaatattc ggccacgcaa attcctcacg gctgcgaata aatccacctg
     4681 cttcaaagtg gttgctggca ccaacgccag tgccgccaaa cagccactcc gcaccgattt
     4741 tcggctggtt ccaccactgc agggcagggt agagggaaac cggttctttg cactcatatt
     4801 gcagatacat ctccagatga tcctgaagat tttcgccgac gccgggtaat tcatgcacca
     4861 gcggaatatc aaactccgcc agcagttcag cgttgccgac gccggagcgt tgcaggatct
     4921 gcggtgaggc aatcgcgcct gcacataaca gcacttcttt gttggccgtt gcgcgggttg
     4981 ggatggtgct gtcgccttcc agccattcga cgcccaccgc gcgtttgccg tcaaaaatga
     5041 tgtgatcggt catagcgtga atacgaatgg tcaggttagg gcgcgatttg gcctgatcga
     5101 gataaccgcg cgcggtactg gcgcgacggc cctgcggcgt gacggtgcga tccatcggac
     5161 caaaaccttc ctgctgataa ccgttgagat cgtccgtgcg cgggtagccc gcctgcacgc
     5221 ccgcttcaat catcgcttca aacagcggat tgacgccggg tttggaggta gtgacgctca
     5281 ccgggccatc gccaccgtgg tagtcgttct cgcccacatc gcgagtctcg gccttgcggt
     5341 agtagggcag gcagtcgaga taactccagt tctccagacc gggttcttgc gcccagttat
     5401 cgagatccag cgcattgcca cggatgtagc acatgccgtt gatcagcgac gatccaccca
     5461 ggcctttacc gcgtccgcac tccatgcggc ggttattcat aaacggttca ggctctgttt
     5521 cataggccca gttgtagcgt ttgccctgta gtgggaacgc cagcgctgcg ggcatctggg
     5581 tgcggaagtc aaagcgatag tccgggccgc ccgcttcaag cagcagcacg gaggtattcg
     5641 gatcttcagt cagacgggta gcgagaacgt tgccggctga gccggcacca ataatgatgt
     5701 agtcaaattg caaataaacc tcctggttag aatatggact ggaatttagc catctcaacc
     5761 tggatggact tcacctgggt gtaactctgg agcgtcatca cgccgttctc gcgaccaatg
     5821 ccggagtgtt tgtagccgcc aacgggcatc tctgccgggg attcgcccca ggtgttgatc
     5881 cagcaaatac ccgcttccag ctgatgaatg acgcgatgcg cgcggttcag gtccgccgtc
     5941 acgatgcccg ccgccaggcc gtagtcggta tcattggcgc ggcgaatcac ttcgtcctca
     6001 gattcataag tgagaagcga catcaccgga ccgaagatct cttcgcgcac gatggtcatt
     6061 tcgtcgcggc aatcggtgaa cacggtcggt gcaacccatg cgccgttatc gaagccatcg
     6121 cctttcagta catcgccgcc gcacagtacg cgcgcgcctt cctctttgcc tttggcgata
     6181 tagcgcagca cgttatcgcg atgcgggaag ctgaccagcg ggctgaagtt agtttgcgga
     6241 tcgaaaacgt cgcccgcgcg aatgcgctca acgcgcgcca gaattttctg ctcaaatgcg
     6301 gctttgcatt tcgccggaac gaagacgcgg gtgccgttgg tacacacctg accggagctg
     6361 aagaagttcg ccatcatggc gatatcggcg gcgagatcga gatccgcatc gtcgaaaacg
     6421 atcagcggtg atttaccgcc cagttccatg gtcacttctt tcagggaaga ggccgccgag
     6481 ttagccatca cttttttgcc gctggcgaca ccgccggtaa atgacacttt ggcaatgccc
     6541 ggatgctcgg tcagatattg cccggtctcc gcgccccccc ccggcaacac gttaaatacg
     6601 ccgtccggca ggcccgcttc gctgtaaatt tcagccagct ttaacgcggt aagcggggtg
     6661 acttcgctcg gtttgaaaat cattgcgttg cctgccgcca gcgccggggc ggatttccac
     6721 agggcaatct ggatcgggta gttccatgcg ccaatccccg ccaccacacc cagcggttcg
     6781 cggcgggtat aaacaaatga cgtctcacgc aacgggatct ggctgccttc cagcgccggg
     6841 atcagcccgg cgtagtactc cagcacgtcc gcaccggtaa cgatatcgac ggttgaggtt
     6901 tccgaatatg cttttccggt atcgagggtt tccagttttg cgagttcgtc attgcgttca
     6961 cgcagaatat caacggcccg acgcagaata cgcgagcgct ccatagcggt catcgccgcc
     7021 cagatttttt gcccctgttg ggcactcttc acggcgcgat cgacatcctc acgcccggcg
     7081 gcctgcacgg tcgccagcac gttaccgttg gccgggttaa tggtctcgaa ggtgcgaccg
     7141 ctggtggcgg aggtataacc accatgtata taaagctgct gttctgccat tcgggacatc
     7201 acgtctcctc ggttaatcgg tgggtaaatg ctgagtaata aagtgacggg tcagcgagtg
     7261 tgcgagaggt ttatccagcg gtttgccgct cagtgccgcg cgcagccata atccgtcaat
     7321 cagcgcggca aggccgtagc ccgcttcctg tgcctgttgg cgcggtaact cgcggcgaaa
     7381 ttcgctcacc agattcgaca ataagcggcg gctactgacc tgctgtaaac gatagagcat
     7441 cggctgatgc atactgctgg cccagaacgc cagccaggct ttcatcgccg cactgctcac
     7501 ctgcgtttca tcgaagtttc cgccaacaat cgcctgtaat cgctgctctg cactgccctg
     7561 cggaagtgca tgtaatcgat tcaaaaccgc gtcacgcagc tgactggtga tatcgcgcat
     7621 ggttgcttcc agcagaccat ttttgtccct gaaatagtgg ctgatgatcc ccgtagaaac
     7681 gcctgcacgg cgggcgatct gcgcgatcgt tgcatcgtgc atgcccactt catttattgc
     7741 ttccagtgtg gcgtcgatca gttgtctgcg ccggatcgac tgcatcccca atttgggcat
     7801 tttcgccact ccattcatca gcgttgttta tctattaaag cggttattga ttggacgttc
     7861 aatataaaat gtgtcttaat tgttacgaat ttgattttaa atggtaacaa taacagtggg
     7921 gatactggat gacagacctt tcacacagca gggaaaagga caaaatcaat ccggtggtgt
     7981 tttacacctc cgccggactg attttgttgt tttccctgac aacgatcctg tttcgcgact
     8041 tctcggccct gtggattggc cgcacgctgg actgggtttc taaaaccttc ggttggtact
     8101 atctactggc ggcaacgctc tatattgtct ttgtggtctg tatcgcttgt tcgcgttttg
     8161 gttcggtgaa gctcgggcca gaacaatcca aaccggaatt cagcctgctg agttgggcgg
     8221 cgatgctgtt tgctgccggg atcggtatcg acctgatgtt cttctccgta gccgaaccgg
     8281 taacgcagta tatgcagccg ccggaaggcg cgggacagac gattgaggcc gcgcgtcagg
     8341 cgatggtctg gacgctgttt cactacggct taaccggctg gtcgatgtat gcgctgatgg
     8401 gcatggcgct cggatacttt agctatcgtt ataatttgcc gctcaccatc cgctcggcgc
     8461 tgtacccgat cttcggtaaa cggattaacg ggccgatagg gcactcagtg gatattgcag
     8521 cggtgatcgg caccatcttc ggtattgcca ctacgctcgg tatcggtgtg gtgcagctta
     8581 actatggctt gagcgtactg tttgatattc ccgattcgat ggcggcaaaa gcggcactga
     8641 tcgccttgtc ggtgataatc gccacgatct ctgtcacctc cggtgtcgat aaggggattc
     8701 gtgtgttatc ggaacttaac gttgcgctgg cgctgggatt gatcctgttc gtattgttta
     8761 tgggcgacac ctcgttcctg cttaatgcgc tggtgctgaa tgttggcgac tatgtgaatc
     8821 gctttatggg catgacgctc aacagttttg ccttcgaccg tccagttgag tggatgaata
     8881 actggacgct cttcttctgg gcatggtggg tggcatggtc gccgtttgtc ggcttgttcc
     8941 tggcgcgtat ctcgcgtggg cgtaccattc gccagttcgt gctgggcacg ttgattattc
     9001 cgtttacctt cacgctgtta tggctctcgg tgttcggcaa tagcgcgctg tatgaaatca
     9061 tccacggcgg cgcggcattt gccgaggaag cgatggtcca tccggagcgc ggcttctaca
     9121 gcctgctggc gcagtatccg gcgtttacct ttagcgcctc cgtcgccacc attactggcc
     9181 tgctgtttta tgtgacctcg gcggactccg gtgcgctggt gctggggaat ttcacctcgc
     9241 agcttaaaga tatcaacagc gacgcccccg gctggctgcg cgtcttctgg tcggtggcga
     9301 ttggcctgct gacgctcggc atgctgatga ccaacgggat atccgcgctg caaaacacca
     9361 cggtgattat ggggctgccg ttcagctttg tgatcttctt cgtgatggcg gggttgtata
     9421 aatctctgaa ggtagaagat taccgccgtg aaagtgccaa ccgcgatacc gcaccgcgac
     9481 cgctggggct tcaggatcgc ctgagctgga aaaaacgtct ctcgcgcctg atgaattatc
     9541 cgggcacgcg ttacactaaa cagatgatgg agacggtctg ttacccggca atggaagaag
     9601 tggcgcagga gctgcggttg cgcggcgcat acgtggagct aaaaagcctg ccgccggaag
     9661 agggacaaca gttggggcat ctggatttgt tggtgcatat gggcgaagaa caaaactttg
     9721 tctatcagat ttggccgcag caatattcgg tgccgggctt tacctaccgc gcacgtagcg
     9781 gtaaatcgac ctactaccgg ctggaaacct tcctgttaga aggcagccag ggcaacgacc
     9841 tgatggacta cagcaaagag caggtgatca ccgatattct tgaccagtac gagcggcacc
     9901 ttaactttat tcatctccat cgtgaagcgc cgggccatag cgtgatgttc ccggacgcgt
     9961 gattgttttt gcataaaacc gcgccatgtc tgcatatggc gcgggaatca ttgactcgct
    10021 gtaaaaaatt atgcatatat tatatatgat tgtcatgtca tatcctgcaa ttcctcttgt
    10081 atcacttcat gattttatat tttattaatt aacgatttta ttttgtatta gctgaatgga
    10141 ttcagtccta tagtgttata tatttattaa taagacgatt cttatatttt gatacctaat
    10201 aagagttacc gcttttatcg gctgtatatt aaatgtattc ctcttgcttt tgtttatatt
    10261 ttgagttgat ttgagttcct ggatacatat aatgcgcagg tattagagac tgggaggaat
    10321 gtaatgataa agttaagagt gttaacttaa aataagtttt aagtaataac ttgctatttt
    10381 attaccctct gaactacata ctcagatatg tggttgccaa tgctatgcaa aatttttgaa
    10441 tttttatatg cgatgaacag taaccagaat ggcatttaat gcgttgctgt ttatgcaatc
    10501 ttggttttat ttggatgtat tgctggagat tgtgatgaat aaaatatatc ggctaaagtg
    10561 gaacaggtcc cgtaactgtt ggagcgtctg ctctgagctg gggagcagag taaaaggaaa
    10621 aaagtcccgg gctgttttaa ttagcgcgat aagtttatat tcatctctgg tattcgccga
    10681 tgatgtcatc gtaaaccagg ataaaactat tgattttggc aaagagaacc agagcatcga
    10741 ttaccgtatt acggtgactg acaatgccaa tctggtaatc aatacgacag atacttcccg
    10801 tccgcgtctg actctcgctt ctggtggtgg gttggatatt accggaggaa aggtaacaat
    10861 caatggcccg cttaactttt tgctgaaagg tacggggttc ctgaatgtct ccaatgctgg
    10921 cagcgagtta tatgctgatg atttgtatga gtcaaactca ggcatgagac atgatcaagg
    10981 ctattttaat gtctccaatg gcggcaaaat tcatgttaag ggcaccagtc gtctgactta
    11041 tacgcagggg aatgtaagcg gcgaaggtag tcaggtaaat gcagggacct tctttatggg
    11101 cgtttacggc ggatatggcg gtaatcagtt cctgtcggtt aataacggtg gtgaagttaa
    11161 tgctcgtgaa catattagcc tgggttatta cgaccaacgt tctgatacaa cattagtcgt
    11221 atcagatgga ggtaaaatat ccgcaccgaa aattagttta agcaccaact ctgagttagc
    11281 gttaggtgca caggaaggaa gtgctgcgaa ggctgcaggg attattgatg ccgaaaaaat
    11341 tgagtttgtg tgggcaaaga catccgataa gaaaatcacc ttaaaccaca cggataaaaa
    11401 cgcgactatt tccgcggata ttgtcagtgg cagcgagggc ctgggctata tcaatgcgct
    11461 caatggcacg acttacttaa ccggtgataa ctctgccttt agtggtaaag tcaaaattga
    11521 gcaaaatggc gctttaggga tcaccaaaaa tataggtaca gcagagatca acaaccgcgg
    11581 gaaattacac ctgaaggctg acgatagcat gacctttgcc aataagattt ccggcaatgg
    11641 tacaataagt atcgacagtg ggacagtggc actgaccggc aataactatg cattcagcgg
    11701 atatattgat gtcgcttctg gtgctgtcgc ggttatttct gaagacaaga acatcggccg
    11761 tgcggacctg gatgttgatg gcaaactgca aatcaatgcc aataaagatt gggtatttga
    11821 taacgatctt caaggtagag gtattgttga aataaacatg gggaatcacg aattctcctt
    11881 cgatgagttt gcttatacag actggttcca gggttcactg gcgttccaga acacgacatt
    11941 taacctggaa aagaatgctg agtttctgca gagaggcggg atcactgcgg gtcagggaag
    12001 ccaggtaaca gtgggtaagg gcgctcactc cattagcact ttgggattct ccggcggaac
    12061 cgttgatttt ggtgccctga cagcaggtgc acagatgaca gaagggacgg tcaacgttag
    12121 taaaacgctg gatttgcgcg gcgagggtgt gattcaggtt tctgacagtg acgttgtccg
    12181 ctcagtatct cgtgatattg actctgcgtt atcgctcact gaagtcgatg atggtaacag
    12241 cgccattaag ctggttgatg cgcaaggtgc ggaagttctg ggcgatgcgg gcaatctgca
    12301 attgcaggat aaaaatgggc aaatcctctc cagcagcgcc caacgtgata ttcagcagaa
    12361 tgggcaaaaa gcggccgtcg gcacttacga ctatcgtctg acgagtgggg taaacaatga
    12421 cggtctgtat atcggttacg gcctgaccca gcttgattta cacgctaccg acagcgatgc
    12481 tctggtgctg agctctaacg gtaaaagcga gaatgccgcc gatctcagcg caaagattac
    12541 cggcagtggt gacctggcat tcagcagcca gaagggtcag accgtatcgc tttctaacaa
    12601 agacaacgac tataccggtg ttaccgatct gcgcagtggg acgcttttgt tgaataacga
    12661 taacgtgttg ggtaataccc atgaactgcg tctggcggca gagactgaac tggatatgaa
    12721 tggtcacagc cagaccgtgg gcacgctcaa tggcagcgcc gattcactgc tgagcttaaa
    12781 tggcggcagt ctgacggtta ccaacggggg cacttcaacc ggttcgttaa cggggagcgg
    12841 agagctgaat attcagggcg gcacgctgga catcgcgggc gataacagca acctgacggc
    12901 gaatgtgaac attgctaatt cggctaatgt cctggtaagt catgcgcagg gattgggtag
    12961 cgcaaacgtt gagaacaacg gtaccctggc gttgaataat agcactgaaa aaagagcggc
    13021 tgcgtctgtg aattacaccc tgggcggcaa tctgaccaac aacggtacgc tgatgaccgg
    13081 aatgtcagga cagcaagctg gcaatgtgtt agtggtgaag gggaactacc acggtaataa
    13141 cggtcaacta gtaatgaata cggtactgaa tggcgatgac tcagtaaccg ataaattggt
    13201 tgtcgagggc gatactagcg gcacgactgc cgttacggtg aataacgctg gcggtacagg
    13261 tgcgaaaacc cttaacggta tcgaacttat ccatgtagac ggtaagtctg agggcgaatt
    13321 tgttcaggct ggtcgtatcg ttgcgggggc gtatgactac actctcgcgc gtggacaagg
    13381 ggcaaatagt ggtaactggt atctgaccag cggcagtgat tctcctgaac tgcagccgga
    13441 gccagacccg atgccgaatc cggagccaaa cccgaatcca gagccgaacc ctaacccgac
    13501 acctacgccg ggtccggatc tgaatgtgga taatgacctg cgaccggagg cgggtagcta
    13561 cattgcgaac cttgcagcag cgaatacaat gttcaccacg cgtctgcatg agcgtctggg
    13621 taatacgtac tataccgaca tggtgacggg tgagcagaaa caaaccacta tgtggatgcg
    13681 ccatgaaggt ggtcataata aatggcgtga tggcagcggc cagctgaaaa cccaaagcaa
    13741 tcgctatgtt ctgcaactgg gaggcgatgt cgcgcagtgg agccaaaacg gcagcgacag
    13801 ctggcatgtt ggggtcatgg cgggatatgg caacagcgac agcaaaacca tttcctcgcg
    13861 aaccggttat cgtgcaaaag cgagtgtgaa cggatatagc accggcctct atgccacctg
    13921 gtatgccgat gacgagtcgc gtaatggcgc gtatctcgac agttgggcgc agtacagctg
    13981 gtttgataac acagtgaaag gggatgactt acaaagtgaa tcctataaat caaaaggatt
    14041 taccgcttca ctggaagctg gatacaaaca caaattagct gaatttaatg gcagccaggg
    14101 aacgcgtaat gaatggtatg ttcagccgca agcacaggtt acctggatgg gagtcaaagc
    14161 cgataagcac cgcgaaagca acggaaccct cgttcatagc aacggtgatg gcaatgttca
    14221 aacccgactt ggcgtaaaaa cctggctgaa gagccaccat aaaatggatg acggtaaatc
    14281 ccgcgagttc cagccgtttg tagaagtgaa ctggctgcat aacagtaagg atttcagcac
    14341 cagtatggat ggcgtgtccg tcactcagga tggagcccga aatattgctg agataaaaac
    14401 cggggtggaa ggacagctaa atgccaacct gaatgtctgg gggaatgtgg gcgttcaggt
    14461 tgccgatagg ggatataatg acacctctgc aatggttggc attaagtggc aattctgatc
    14521 tgaataatga gtaaaaggag agagagccga acgggagata attcggctct ctgtgtcatt
    14581 ttcttgcagc cagctttatc agtcagagtg aagactcgca gatttagctg gcgcacaaga
    14641 tttaatcaag gtgtgttcaa tggcttcgca tgatttgagt gtttttttgg aagagttcgg
    14701 ggcaacggtg aatttgacgt tgcctggtat cgtgtccgaa aaagaacggc ttttactcaa
    14761 gctgctaatg gagggaatgt cggttacaga aatctcacag tacagaaatc gcagtgcaaa
    14821 gacaatttca catcaaaaga agcagctgta tgaaaaacta ggcatccaga gtgatattac
    14881 cttctggcgg gatatctttt ttcaatatca tccgcaagtc atcagtggca cgggaaataa
    14941 aaataatttt tatatccctg ataaccgttg tcaccatatc gtcacgcctg aagccatcag
    15001 tctggcgttg gaaaaccacg aattcaaacc gtggatccaa ccggttttct gcgcgcagac
    15061 tggggtactg acgggctgtg aggtacttgt ccgctgggaa catccacaaa cgggaattat
    15121 cccaccggat cagtttattc ctctggcgga gtcatccggt cttattgtca taatgacccg
    15181 ccaactgatg aaacagactg cggatattct gatgccggta aaacatttgc tgccggacaa
    15241 tttccatatt ggcatcaacg tctcggcggg ttgttttttg gcagcgggat ttgaaaaaga
    15301 gtgtctgaac ctggttaaga aattaggtaa cgataaaatc aagctggttc tcgagctaac
    15361 ggaacgtaac cctattccgg taacgccaga agccagagcg atatttgaca gccttcatca
    15421 gcacaacatt acctttgcgc tggatgactt tggtacgggt tatgcgacct atcgttactt
    15481 gcaggcgttc ccggtcgatt ttattaagat cgataagtca tttgtgcaaa tggcgagtgt
    15541 cgacgaaatc tccggtcata ttgtggacaa tattgtcgaa ctagcgcgta agcctggtct
    15601 gagtatcgtg gcggaagggg tagaaaccca ggagcaggcg gatttaatga tcggtaaagg
    15661 cgttcacttt ttgcagggct atttgtactc tccgccagta ccgggtaata aatttatctc
    15721 tgaatgggta atgaaagcag gtggttgatg taaaccgcta ttcacagcgc atcgggaggt
    15781 tggcagcgat taattctccc gatgcagtaa atcctgatag atccccgtta atatgccatt
    15841 tggcgcaaat tgttttttaa tccactgcgt cacctgaccc gtagcagaat gctgagtcgc
    15901 cagtaacatg cgcgaatcct ggcgcggatt atggatttgt cgggtaacca gcagggattg
    15961 cgtcatcgcc tcacggacca tgtaatccgg caaaaaacca attccttcac ctaaaatctg
    16021 gcactgacat ttggtgttga aatctggcac cagaattgac tcctgcccgt gcagcaacca
    16081 gcccactttt ttattaatcg tatgcgcggt gtcctccacc ataatattag ggtagagacg
    16141 cagttggctt tcggcgatgg gttccggcac aaaggctaac gggtgatccg gggcgatagc
    16201 aaaagcccag cgaatcgcgc caatttcagt gtaatcaata ccgccgccgt ccagtagtgt
    16261 atcgggtgcg ccgatggcga tattggcctg attattaata atcgcatccc agacgccgtt
    16321 atacacttcg gtggtgacgg taatctggca ggtaggaaac tgctttttca gcacctgtaa
    16381 caagcgtgcg gtgtgtttgg gcgtataaag cagctgatta atacaaatgc gcacacgcgc
    16441 ttctatgccc tgcgaaatag tatcgatact gcgtttgatg gcataaaaat cattcagcag
    16501 gtcggtagct ttgcggaaaa aataacgccc ggactccgtt aactcaatgc tgcgggtact
    16561 gcgagtgaac agcaccacat ccagccccgt ctccatacgc ttgatggtgt agctaatggc
    16621 ggaagtggtt aaacccaact cttctgcggc tttactgaag ctgccaaaac gcgcagcggt
    16681 agtaaatgcc aacagattct cttcggtaaa aattgagttc attaaaccat tctcgccatt
    16741 atctactatg aatttctcca gctatgactg tagaggtatc ggttaaagat agataatcat
    16801 ttttgaataa cttttaatac ccgtcgcgtt tagtcgtcgc ttcgcttaat ttttgtccac
    16861 catattctga aattatcccc acgcagttac ccgccagaaa acccagtaac gtcaccgtcc
    16921 agttcatccc gctggcaaaa aacagcgtca tgccgagaaa gccaccggga ataaaggaca
    16981 gtaaccagaa acgcccttgc cagaccacta ccgccgagaa gggtacggta gtaattacgc
    17041 tcgcccagaa cgcggacagt ccacttacag aggccagcca accggcagcc agcgcggcga
    17101 aaaatgccca caccatcccg gaataattaa ccgccagact tctgataaac cccgatttac
    17161 cgccgcctgc ggcataaaaa ctgcaaaagg cgacgaagcc aatggttcct aataattccc
    17221 atccctgaga gagcccgaca tgtgaagaca ctaactgcca aagccctgca caaataccga
    17281 cggtgacacc cgttgccgtg agtccgttca tcttgttccc ctctggttag acatggacga
    17341 aagctactct tacatgtgca gactatagag aaataaaacc tgattgagtg aaagggtatc
    17401 gggtcaaaga aacaaatatt gcactaccgc acactgcgaa aagattgttg aacatcattc
    17461 aacaaaaagg cgaaatacag cgaattaaat ttaagcgggt tcttttatca gattatcgtt
    17521 gctattctca attcatcgaa aacagccaca aaaaccaggg gaatgtgaat gagtataaaa
    17581 aatctacctg ccgactattt attggctgca caacagggtg atatcgataa agtaaaaacc
    17641 tgccttgcgc tgggtgtcga tattaatacc tgcgatcgtc aggggaaaac ggcaattacg
    17701 ctggcaagtt tatatcagca atatgcttgc gttcaggcat taattgatgc cggagcggat
    17761 attaataagc aagatcatac ctgtttaaat ccttttttaa ttagctgtct gaacgatgat
    17821 ttaacgctac tacgaattat tttaccggct aaacccgatc ttaattgcgt aacccgtttt
    17881 ggcggtgtcg gcctgacgcc tgcctgtgaa aaaggccatt taagtattgt aaaagagctt
    17941 ttggcgcata cggagattaa cgttaaccag accaaccatg taggctggac gccgctactg
    18001 gaagcgattg tgcttaatga tggtggtatt aaacagcagg cgattgtgca gttattgctg
    18061 gaacacggtg ccagcccgca tctgaccgat aaatatggca aaacgccact ggaactggcg
    18121 cgggaacagg gctttgaaga gattgcgcag ttactgattg ccgcaggtgc ataaaccggg
    18181 aggcttgcta tcaacacacc agaaagacgg tgtgtgtggg cgctaactgc ggatgcggat
    18241 tttctggcgc agcgggggca aggacaggtt gaacaggtct ttgccagagc ggtaaatatc
    18301 gcactcccgg ctcgccagca gttgctgacg ctgctttgtg aagagtacga caatgcgcca
    18361 aacagttgtc ggttggcact cactcacttt gatgatctgt tccggcatgg tgataaggtt
    18421 cagtttgacg atcaaggtat tacggttggt caacatcttc atatagagat gagtcgttgt
    18481 cggcgttggc tgtccccaac cttgcaaatg accgctgtga attttcacct tatcgcctgg
    18541 ctacagtggc acgacattat tcatcagcac ctgggggaaa atgaaaccct gtttaattat
    18601 cgcggcgata atccgtttta tcaggcgtta aataaagaac tacatattaa acgacgggca
    18661 gttattcagg cagtaaacga aaaacaaaat atcgccgcag cggtcgccag tatgattggg
    18721 ttagggattg gccttacgcc atcagccgac gattatttaa caggtctggc gcttatttta
    18781 tttattcccg ggcatccgtc ggaaaaatac aaagaggaat tttatctcgg tatgcagcgc
    18841 ggcagaaata acaccacatt attaagtgct ataacgctgg aagccgcatt acaacaacgt
    18901 tgccgggaaa atattcatcg ttttattcac aacattattt atgacatccc tgggaacgca
    18961 actcaggcaa tagaaaaaat taaacatatt ggctccagtt ccggctgcga catgctgtat
    19021 ggcatggccg atggttgtgc gctgagccaa acctacggag ggaattatgt cagttaaaat
    19081 agtcattaaa ccgaatacct attttgattc tgtctcgctg atgtctatct ccacgcgtgc
    19141 aaataaactc gacggcgtcg agcaggcatt tgtggcgatg gcgaccgaaa tgaacaaagg
    19201 tgtactgaaa aatttaggac tgctgacgcc ggagctggaa caggcgaaaa acggcgacct
    19261 gatgattgtc atcaatggta aatcgggtgc ggacaacgag cagttgctgg tggagattga
    19321 agaactgttc aacaccaaag cgcaaagcgg ctcgcacgag gcgcgttacg ccactattgc
    19381 cagcgccaaa aagcatatcc cggaaagtaa cctggcggtg atttcggtca acggtctgtt
    19441 tgccgctcgc gaagcgcgtc aggcgctgca aaacgatctc aacgtgatgc tgttttccga
    19501 taacgtctca gttgaagatg aactggcgct caagcagttg gcccacgaaa aagggctgct
    19561 gatgatgggg ccggactgcg gcacggcgat tattaacggc gcggcgctct gttttggtaa
    19621 cgccgtgcgt cgcggcaata tcggtattgt tggcgcatcc ggcaccggca gccaggagct
    19681 gagcgtccgc attcatgaat ttggtggcgg tgtttcacaa ctgattggca ccggcgggcg
    19741 cgacctgagc gagaaaatcg gcggcctgat gatgctcgac gccatcggga tgctggaaaa
    19801 cgatccgcaa actgaaatta tcgcactgat ttcaaaacct cccgcgcctg cggtggcccg
    19861 caaagtgctg gaacgcgcac gtgcctgccg caagccggtg gtggtctgct tcctcggtcg
    19921 cgttgaaacg ccggtggatg agcaggggct acagtttgcc cgcggcagca aagaggcggc
    19981 gctaaaagcg gtgatgctct ccggcgtgaa acaggaaaat ctcgacctgc atacgcttaa
    20041 ccagccgttg attgctgatg tgcgtgcgcg tctgcaaccg cagcagaaat acattcgtgg
    20101 cctgttctgc ggcggcacgc tgtgcgacga aaccatgttc gcggtgatgg aaaaacatgg
    20161 cgatgtctac agcaacattc agcccgatcc ggaattccgc ctgcaagata tcaaccgcag
    20221 catcaaacac accttcctcg actttggcga tgacgacttc acgaacggca agccgcatcc
    20281 aatgattgac cccaccaacc gcatcagtcg cttgatcgaa gaggcgcgcg atccagaagt
    20341 ggcggtgatc gtgatggatt ttgtgctcgg atttggatcg catgaagatc cggtcggctc
    20401 caccatcgag gcgatcaaag aagcgaaagc gatcgccgct gccgaaggac gcgagttgat
    20461 cattctcgcc tatgtgctgg gtaccgatct tgatacgcca tcgttagaac aacaaagcca
    20521 gatgctgctt gatgccggag tgattctggc gagcagcagc accaataccg gattgctggc
    20581 gcgtgaattt atctgcaaag gggaggaagc ctgatgagcc agtcactgtt tagccaacca
    20641 ttgaacgtta ttaacgtcgg catcgccatg tttagcgatg acctgaaaaa gcagcatgta
    20701 gaagtgactc aactcgactg gacgccgccg gggcagggca atatgcaggt ggtgcaggcg
    20761 ctggataaca ttgccgattc gccgctggca gacaaaatcg ccgccgctaa ccagcaggcg
    20821 ctggagcgca ttatccagtc gcatccggtg ctgattggtt ttgatcaggc gattaacgtg
    20881 gtgccgggca tgacgccgaa aaccattctt cacgccgggc cgccgatcac ctgggaaaaa
    20941 atgtgcggcg cgatgaaagg cgcggtcacc ggggcgttgg tgttcgaagg gctggcgaaa
    21001 gatctcgacg aggcggctga actggcggcc tccggggaga tcaccttctc gccgtgccac
    21061 gaacacgact gtgtgggatc gatggcgggt gttacctctg cctcgatgtt tatgcacatc
    21121 gtaaaaaaca aaacctacgg caacatcgct tacaccaata tgagcgagca gatggcgaag
    21181 attttgcgta tgggagccaa cgatcagagc gtgatcgacc gcctcaactg gatgcgcgat
    21241 gtgcaggggc caatgctgcg cgacgcgatg aaaattatcg gcgagattga tctgcgctta
    21301 atgctggcgc aggcgctgca tatgggcgat gagtgccata accgcaataa cgccgggacg
    21361 acgttgctga ttcaggcgct gacgccggga attattcagg caggttattc cgtggagaaa
    21421 cagcgcgaag tgtttgagtt tgtcgctagc agcgactact tctccggccc gacgtggatg
    21481 gcgatgtgta aagcggcgat ggatgcggcg cacggcatcg aatacagcac cgtggtcacc
    21541 accatggcgc gtaacggcgt cgagttcggc ctgcgggtca gcggattacc ggggcaatgg
    21601 tttaccggtc cggcgcagca ggtcatcggc ccgatgtttg ccggttataa gccggaagat
    21661 tcggggctgg atatcggcga cagcgccatc actgaaacct acggtattgg cggatttgct
    21721 atggcgaccg cgcctgcaat cgttgcgctg gtgggcggca cggtggaaga ggctatcgat
    21781 ttctctcgcc agatgcgtga aatcactctc ggtgaaaacc ccaacgtcac cattccgctg
    21841 ctcggtttta tgggcgtgcc gtcggcaatc gacatcaccc gcgtgggtag cagcggcatt
    21901 ctgccggtga tcaacactgc catcgcccat aaagatgcgg gcgtcggcat gattggcgcg
    21961 ggcattgtgc atccaccttt tgcctgcttc gagaaagcca ttcttggctg gtgcgaacgt
    22021 tacggcgtct gacctacatg ttcatgccgg atgcggcgtg aacgccttat ccggcctacg
    22081 gatggcccga gaattgtagg cctgataagc gtagcgcatc aggcagtctg gcgttggtca
    22141 taacctaatc accctctgta gcggacataa caacatgaaa gagcttgtgg tcgttgccat
    22201 tggtggcaac agcattatca aagataacgc cagccagtcg attgagcatc aggcggaggc
    22261 ggtgaaagcc gtggccgata cggtgttgga aatgctggct tccgattacg acattgtgct
    22321 gacccacggt aacgggccgc aggtcgggct ggatttacgc cgtgcggaga ttgcccacga
    22381 gcgcgaaggg ctgcccttaa cgccgctggc gaactgtgtg gcagatacgc agggcggcat
    22441 cggctatctg atccaacagg cgctgaataa ccggctggcg cgtcacggcg agaaaaaagc
    22501 cgtcaccgtg gtaactcagg tggaagtgga taaaaacgat ccaggttttg cccatcccac
    22561 caagcccatt ggtgcattct tcagtgaaag ccagcgtgac aaactacaaa aggcaaaccc
    22621 tgactggtgt tttgttgaag atgccggacg gggctatcgc cgcgtggtcg cctcgccgga
    22681 accgaaacgt attgtcgaag cacccgccat taaggcgctg atccaacaag gttttgtcgt
    22741 aattggcgcg ggcggcggtg gaattccggt agtgcgtact gaagcggggg attaccaaag
    22801 cgtggacgcg gttattgaca aagatctctc taccgcgctg ctggcccgtg aaattcacgc
    22861 cgacattctg gtgatcacca ccggcgtcga aaaagtctgt atccactttg gtaaaccgca
    22921 gcagcaggcg ctggatcggg tggatattgc caccatgact cgctatatgc aggaagggca
    22981 ttttccgccc ggcagcatgt tgccaaaaat catcgccagc ctgacgttcc tggagcaggg
    23041 cggcaaagaa gtgattatca ccacgccgga atgcctgcct gcggcgctgc gcggtgaaac
    23101 gggcacccat attattaaaa cgtaaggacg taggatgaaa gaaagcaata gccgccgtga
    23161 atttctgagc cagagcggta agatggtcac cgccgccgcg ctgtttggta cctctgtgcc
    23221 gctcgcccat gcggcggtag ctggcaccct aaactgcgaa gcgaacaaca ccatgaaaat
    23281 cactgacccg cattactatc tcgataacgt gctgctggaa accggttttg actacgaaaa
    23341 cggtgtggcg gtgcagaccc gcacggcgcg ccagaccgtg gagattcagg acggcaaaat
    23401 tgtcgccctg cgcgagaaca agcagcatcc ggacgccacg ctgccgcact atgacgctgg
    23461 cggtaagttg atgctaccca ccactcgcga catgcatatt catctcgaca aaaccttcta
    23521 cggcgggccg tggcgctcgc tcaatcgtcc ggcaggcacc actatccagg acatgatcaa
    23581 gcttgagcag aaaatgctgc cggaactgca accgtacacg caggaacggg cagaaaaact
    23641 gatcgatcta ttgcagtcga aaggcaccac cattgcccgc agccactgca atatcgaacc
    23701 ggtttccggc ctgaaaaatc tgcagaattt gcaggcggtg ctggcgcgac gtcaggcggg
    23761 cttcgagtgt gaaatcgtcg ccttcccgca gcacggtttg ctgctgtcga aatcggaacc
    23821 cttaatgcgc gaagcgatgc aggcgggtgc gcattacgtc ggcgggctgg acccgaccag
    23881 tgtcgatggc gcgatggaaa aatccctcga caccatgttt cagattgcgc tggactacga
    23941 caaaggtgtc gatattcacc tgcacgaaac cactccgtcg ggcgtggcag ccatcaatta
    24001 tatggttgaa acggtagaga aaacgccgca actgaaaggt aagctgacca tcagccacgc
    24061 ctttgcgctg gctacgctca acgaacaaca ggtcgatgcg ctggcgaatc ggatggcggc
    24121 gcagcaaatt tctatcgcct cgacggtgcc gattggcacg ctgcatatgc cgctcaaaca
    24181 gttgcacgac aaaggcgtaa aagtgatgac tggcactgac agcgttatcg accactggtc
    24241 gccttatggt ctgggcgaca tgctggaaaa agccaatctc tacgcgcagc tctatattcg
    24301 tcctaacgaa cagaacctct cccgttcgct gtttctagcc actggcgatg tattgccgct
    24361 gaatgaaaaa ggcgagcgtg tatggccaaa agcgcaggat gatgccagct ttgtgctggt
    24421 ggacgcctcc tgttccgccg aggcggtggc gcgtatctcg ccgagaaccg caacgttcca
    24481 taaagggcaa ctggtgtggg ggagtgtggc aggttgatgc gcatttgaaa cgcctgcaac
    24541 ggtgaattgt aagagattta agccccaggg agtggggcgg gaatggtttg ctaatgaatg
    24601 atcctgaact ttataatctc gcggcataat ttccagggga ttatttgaga gaaatacaca
    24661 aaagtgagtt ttaacaaagg tatttatcag gccataaatg tgacctgctt cggataaaaa
    24721 agattcagta tcgaaaaacc aatgatactc ttgctgatgg ttaccaacag taattaagat
    24781 gtgatttgtt tttccgtaat cgcaaaaata ttgccatgac cataaaagaa aaattctcac
    24841 aaaaatatcc ccatgcttcc ttttgtactt ttggcgattc agcagcgttg gccgatcacc
    24901 tcgcaacgtt gattgcgact ggtgttaaaa cagcttcctg tggttcgctg gcgggatgta
    24961 ttgaagacaa cgcatttccg atgattggtg agtataagat tgtagaaaat agccgtggtg
    25021 agcctgtatg tgttatcaga gtgattggcc tgcatttact gcgcttttct gatgtcaccg
    25081 tggagcttgc ccgtaaggaa ggtgaaggtg acctcagtct tgaatattgg cgtaatgaac
    25141 accgtcgttt tttccaggca gaaggaagct attcaccaga aatggacgtt atatttgaag
    25201 aatatgccct tattgacgtt gtgtagataa ctatttatct acgcgaaata aactcacaca
    25261 cataaaatat cgctaatttt gtttattttg ctatttttta cgtgatgaat gtcacactta
    25321 ttgttgctgc ccgttttgtc catatttata accatatttt taatagctgc ctgaaatagg
    25381 gctgtttatt ctgcataaaa atattcgaaa attcttattc tctccagcgc gctaatttgg
    25441 gcgaacactt cctgactacc ctgcaatgag gctgaaatga tgaataaacg ttttgttatc
    25501 aatatggtgt cttcgctgtt attaggcgct gcgctaattt ctgcgccgct ccaggcagcg
    25561 gaaaaagtcg tggtgaatat ctctaaagtc gatggtatgc cgtggtttaa tcgcatgggc
    25621 gaaggtgtgg ttgaggcggg taacgccttt ggtgttaatg cgtcacaggt ggggccttca
    25681 agtactgatg ctccgcaaca agttaaaatt attgaggatt taatcgcccg taaggttaat
    25741 gcaattacta tcgtgccaaa cgatgccaac gtgctggaac cggtattcaa aaaagcgcga
    25801 gatgccggaa ttgtggttct gaccaacgaa tcccccggtc agccaagtgc taactgggat
    25861 atcgaaatca ttgataacga aaaatttgcc gctgaatatg ttgagcatat ggcgaaacgc
    25921 atggggggca agggcggcta tgtgatttac gtgggtagtt tgaccgtgcc gcagcataac
    25981 ttatgggctg acttgctggt gaaataccag aaagagcatt acccggatat gcatgaagtt
    26041 acccgccgca tgccggttgc cgagagtgtg gatgactcac gtcgtaccac actggacctg
    26101 atgaaaacct atcctgactt gaaagcggtt gtctcctttg gttcaaacgg tccgattggc
    26161 gcgggacgtg cggtgaaaga gaaacgggcg aaaaataaag tcgcagtcta tggcatgatg
    26221 atcccttcac aggcggcatc actgattaaa agtggtgata tcaccgaagg gattacttac
    26281 gattcggcct ctgcaggtta cgcactcgca gccgtcgcca gcacgttgct gaagggtgaa
    26341 gagatcaaac ctggattaga aatgcagaat ctgggtaagg ccgacgtaga tatggataag
    26401 cgaattatcc gtttccataa ggtattactg gtcaacaaag acaatatcga ctcactttat
    26461 taatccattt cttcggggcg aaagccccga ttcttattgc caggggatgt tatggaaacc
    26521 ttcctttccc ttcgtcatat caacaaaacg tttcatgcca cgcgagcgct gcgcgatgtt
    26581 tctctcgact ttatgtcggg tgaagtgcac tgccttgccg ggcaaaacgg ctgtggtaaa
    26641 tcgacgttga ttaaaattat gtccggtgtt tatcgcccgg atgagggagc ggaaattacg
    26701 cttggtggga aaaactggtc aaagctgaca cccgccgctt cggtggcgca ggggattcag
    26761 gtgatttatc aggacctctc tttatttcct aacctgagtg tctgggagaa tatcgccgtg
    26821 aatcactatc accacggtct gtttgttaac cgccgtcgtc tgcgtgaggt ggcgcaggcg
    26881 gcaatgacca gtattaacgt cacattgcca ctggatacgc tcgtttctga gctttcaatt
    26941 gcccgctgtc agctggtagc aatttgccgt gcactggcgc aggacgcacg gttgatcgtg
    27001 atggatgaac caaccgcttc gctgacccat caggaagtgc aagggttatt acaggtggtg
    27061 catcaattgc gggaacgcgg gatctgcgtg gtcttcgtca gtcatcgtct ggaagaagtg
    27121 atggaagttt ccgaccgtat ttcagtgctg aaagatggtg agctggtcgg gacttttccg
    27181 gctgcagaga tgaccacaaa acaactcggt ttcctgatga ccggccagga gttcgaatat
    27241 caggtgcgag agttgtggca gggaaaatcc agcaccccgg tgcttgaggt gcgtaattta
    27301 agtcgtcatg gggaatatct aaatattaac ttgcgggtgg aagcgggcga agtggtatcg
    27361 attgttggcc tgcttggtgc ggggcgcacg gaattatgtc tgagtctgtt tggtatgacc
    27421 cgaccggatg ctggcgagat cctcatcaac ggccagccgg ttacactgca tagtaaccag
    27481 gacgctatcc gccacggtat tggttatgtt tcggaagatc gcatgtcgcg cggtttggtg
    27541 atggcgcaat ctattgaaga caacatcatt agcactgttt ttcacaaggt aaaagatcgc
    27601 ttcggctttc tgagtgaaac taaagttcgt gacctggtgg acaggctaat caaggcattg
    27661 accatcaaag tctctgatcc tcatcttccg gtgaatacgc tttctggtgg taacgcgcag
    27721 cgagtatcga tcgccaaatg gctggcgatt gggccacgac tgttgattct cgatagcccg
    27781 acagtcggtg ttgatatcgc caacaaagca ggaatatacg gcattatcag cgatctggcg
    27841 gctcatggca ttgcggtatt gatgatctgc gatgaaattg aagaagcgtg gtatcaaagc
    27901 caccggattt tggtaatgca aaaaggtcag atcacccata gcttcctgcc tgacagcagt
    27961 tctcaggcca ggattgcgga ggtggtaaat ggctgaactg aaaaaacgcc atgaattctg
    28021 gctggcatta ctgattgtgg tgctgtttgt tggccttgcc tggcgcagcg acgagtttct
    28081 gacattcggt aatttgtacg atctcgccaa taactatgcc atgttgacta ttctcgcctg
    28141 tggtttgttt gtggtgttga tttccggtgg aattgatatt tcgtttccag caatgaccat
    28201 cattgcgcaa tacggcatgg tgctgttgct gcaaaaaatt ggtggcaact tcgctgtcgc
    28261 gtttgcactg gcgggctgca tcggcatttt acttggctta attaacgcct tactggttaa
    28321 tcgcctacgg gtgccttcta tcatcatcac tatctcgacg ctgaatattt tctatggcct
    28381 gctgttatgg ttgagtaaag gtgtgtggct gtacgacttt ccgccgtggt ttgagcaggg
    28441 ggttatgttg ttcaagtaca ccgatgctga tggctatgac tatggccttg gtctgccgct
    28501 gatcgccatg attacggtgg tgctgctgac agcgtttatc atgaatttca ccagtgtagg
    28561 gcgcaaaatt tatgcccttg gcgggaaccg cgaatcagcc agtcgcatcg gctttagcgt
    28621 gctgaaactg caacttttcg tctacggcta tatgggattg atgtctggcg ctgcgggtgt
    28681 agtgcagtcg tggacggtga tgactgtcgc ccccgattct cttctgggtt atgagctgac
    28741 agtactggct gcggtggtgc ttggcggcac tagtttgctc ggcgggcgcg gcacgttaac
    28801 cggtactttg ctcggcgtgg tgttgttggc agtgatgcaa aacgggctaa atttattggg
    28861 agtctcgtct tactggcaaa cattgatcac cggcatcatc atcgttgcca gcattagtgc
    28921 cacggcgtgg agtcagcatc agaaccggag tctgctatga aaaaatcgtg gcgtaataac
    28981 gttgagtttt acttgatcgg cctgctggtg ctaactgttg ccgcgttcag cattactatg
    29041 ccggagattt tctggtcgat aagtaatttc cagtccgtcg cttcgcaaat gcccgtgctg
    29101 ggcattctgg cgttggctat ggcggtgacc atgctttgcg gtggcattaa cttgtcaatt
    29161 atagccactg ctaacgcctg ttcactggtg atggcgtggg tggcgactca gtatccaccg
    29221 ggtattgcta cggttgtcgc tacgttactg gcgggcgctg gcgcggcggt gataatcggt
    29281 ttatgtaacg gcgtattgat tgctggtatt cgagtgtcgc caattcttgc aacgcttggg
    29341 atgatgacgt tgctcaaagg gataaatatt ctggtgacag gtggtagcgc cattgccaat
    29401 tatccctcat gggtgctgtg gttgaatcat gcgcaatggt tcggtattcc gctaccgatg
    29461 tggttgtttg ccgcagtggc actggggctg tggatattgc tggagaaaac gccgctcggt
    29521 aaagccattt acctgattgg gtctaacgaa cgagcaacac tttactccgg cattaacact
    29581 cgtcgggtgc tgatttgggt ttatgtcatt tccgccttgc tttgtgcggt agcggcattt
    29641 ttgatgatgt cgaaactcaa ctcggctaag gcctcttatg gcgaatcgta cttgctggtg
    29701 tcaatcctcg cggcagtact cggcggggtt aatccggacg gcggcagcgg gcggataatt
    29761 ggtatggtgc tggcgctgtt tcttctgcaa attattgaaa gcggcttcaa tattctcggt
    29821 ataagcccct acctgacgat ggccttatgg ggaacgttgc tgctctgttt cattcaggtt
    29881 cgcggcatgt tggggctgga tcgggtggtt taaatattgg ctatattcaa tggacgcgtt
    29941 ttgccgcgat gacatatcag gcatcgccaa atacacatag ctaatcagga gtaaacacaa
    30001 tgaagatcaa agctgttggt gcatattccg ctaaacaacc acttgaaccg atggatatca
    30061 cacggcgtga accgggaccg aatgatgtca aaatcgaaat cgcttactgt ggcgtctgcc
    30121 attccgatct ccaccaggtc cgttccgagt gggcggggac ggtttacccc tgcgtaccgg
    30181 gtcatgaaat tgtggggcgt gtggtagccg ttggtgatca ggtagaaaaa catgcgccgg
    30241 gcgatctggt cggtgtcggc tgcattgtcg acagttgtaa acattgcgaa gagtgtgaag
    30301 acgggctgga aaactactgt gatcacatga ccggcaccta taactcgccg acgccggacg
    30361 aaccgggcca tactctgggc ggctactcac aacagatcgt cgttcatgag cgatatgttc
    30421 tgcgtattcg tcacccgcaa gagcagctgg cggcggtggc tcctttgttg tgtgcaggga
    30481 tcaccacgta ttcgccgcta cgtcactggc aggccgggcc gggtaaaaaa gtgggcgtgg
    30541 tcggcatcgg cggcctggga catatgggga ttaagctggc ccacgcgatg ggggcacatg
    30601 tggtggcatt taccacttct gaggcaaaac gcgaagcggc aaaagccctg ggggccgatg
    30661 aagttgttaa ctcacgcaat gccgatgaga tggcggctca tgtgaagagt ttcgatttca
    30721 ttttgaatac agtagctgcg ccacataatc tcgacgattt taccaccttg ctgaagcgtg
    30781 atggcaccat gacgctggtt ggtgcgcctg cgacaccgca taaatcgccg gaagttttca
    30841 acctgatcat gaaacgccgt gcgatagccg gttctatgat tggcggcatt ccagaaactc
    30901 aggagatgct cgatttttgc gccgaacatg gcatcgtggc tgatatagag atgattcggg
    30961 ccgatcaaat taatgaagcc tatgagcgaa tgctgcgtgg tgatgtgaaa tatcgttttg
    31021 ttatcgataa tcgcacacta acagactgaa aaaattaata aatacgctgt ggtttaacat
    31081 attaacttcg ctctccactt aactttttag ttaaggagag cgaaataata tcaaagtagc
    31141 agtaaaacct ataacgtaaa ttcaaattgt taaattaact ccctccagta cacaatactt
    31201 cacacgttag ttatgagcga tttctgatag tgcctggttt aatgagagct ttattatctg
    31261 cgacgtttat ttttatttaa gagagatcgc gatgatatca ttaaaagctc cgcacaataa
    31321 tttgatgcca tatacgcaac aaagcatact taatacggtt aaaaacaatc agttaccaga
    31381 ggatatcaaa agctccctgg tttcctgtgt cgatatattc aaggttttga ttaaacaata
    31441 ttatgattat ccttatgatt gtcgtgatga tttagtcgat gacgacaaac tcattcatct
    31501 catggctgcc gtacgagact gtgagtggtc agacgataac gcactcacca taaatgtgca
    31561 gtttaatgat ttccctggat tttatgactg gatggattac cctgatcacc cggttaagtt
    31621 tgtttttcgc atacttgaaa atcaaaaagg gacagtttgg gtatatgacc aggatgatgc
    31681 gtttcttgat attaaagcaa atgtgcaggc tggacgcttt accgggctta agaaattagt
    31741 gcagtttatc gattcagtgc gaacagattg caaatgtatt cttcttgaac accatatgcc
    31801 tttgctgaga attttcccta aagggaaaga gtgtatgcgt gtggaaaaat ggcttcgcga
    31861 aatgtcgtct attccggaga cagacgcgcc cattaagcag gcgcttgcgc atggattatt
    31921 attacactta aaaaatatcc accctgtttt tcctgaatca ctggtgatgt tattgctctc
    31981 tgttctggac gtaaaaacat acagagatga cgcacgatta aatgaatgga tatcgaatag
    32041 agtgcaggaa ttaggtgaca gatattatcc tgtaaataag cacgttaaaa tccgctacac
    32101 tctttagttc agaaagggtt ccacctgcaa gttgttattc caccagagag tgaatcgcaa
    32161 agcaggtaat catttcatct gtatatccgt atgccaaatg gcattccacc gcctttttcg
    32221 ccgatatttt gttgtgatcg gcgacgcttc gcttaaaaaa gcaccagcag tggtttcgca
    32281 gctatgcggt gtataaaaaa tgatctcatg cagatgtttt gtgaatgtgt gggttgacat
    32341 tcatataaaa aaaatcataa ttccatcatg tttgtgtggg gtcttttctg taccttagca
    32401 tcgcactcaa gcctgacaga aaatatgctg taaggctcat atcaaaaacc gccattagct
    32461 catcaggaag agcagacgac aaccaggatt gttgtatggt acggggttcg aggcctcgat
    32521 ggcggtccaa cttttcaaaa ctcttctgaa tcagatcccc ttattatcat ccctgcaaag
    32581 tggaacattg ccattgtttg gctattcagg acgggtcaga ccaatgaaaa aagcccacac
    32641 aggggagagt gggctgaaat gggaagctaa agactcaagt aaacttatcg gaaataagga
    32701 ccacgcatta cgggggcaat catcgccgat ggtcgggatt cggcaaatca ggttgattac
    32761 gtagagagta aattatctgc tcaccgctgc gtcacccctt cgtaaatcag gcgtagcgcg
    32821 aatacaccaa taattgcacc aataacccga ctggcaacgc gttgcatacg cccataagca
    32881 cgacgcacag cgggcaaaga aaacgcctga ctaagaaaaa ctcgccagat aattgatgcg
    32941 agcacaatcc ccgcccaggc cattaaacgt gcccatgttg gtgtttcggc atttaatgtt
    33001 actgagaaaa tactgataaa aaataaaacg gtttgcgggt tagagagatc ggtaattaat
    33061 ccgcggcgaa aaaagacata ccagggggcg ctaatcggtt gttgtagtgt gctcatttgc
    33121 ggtgttgact ggcggcgcat gctgcaccac gcaaaccata agagataagc gccgccgacg
    33181 attctgataa gcgaaaaaat ctcctcacac tgcgtaatta gcgttgcaag accaaacaaa
    33241 cccaacccgg aataaaatgc atcgcccagc gccacgccca gcccggtcag cacccctgcg
    33301 cgtcgaccgg aagccaggct ggtttgtact accacaaaga gattggctcc cggattaaaa
    33361 aaagtaatca cgaacagtcc tacggtcagg taaacggcgt gcaaaggatc catagtgatt
    33421 tcatccataa ataagtgaag taacttcatc atatttctac caaaaataat cgggtgcgag
    33481 agagatcaca aagtgtctta tttccggtta ctggcgttta tgccctgact gaactaatta
    33541 ttaatcaacc caataatgtg ggtggatgat agtgtgataa caactctgga gccgtaatat
    33601 gaaaataatc tctaaaatgt tagtcggtgc gttagcgtta gccgttacca atgtctatgc
    33661 cgctgaattg atgaccaaag cggaatttga aaaagttgaa tcgcagtatg aaaaaatagg
    33721 tgatatttca actagcaatg aaatgtcgac tgcagacgcg aaagaagatt tgatcaaaaa
    33781 agcggatgaa aaaggggctg atgtgttggt actgacctcc ggtcaaactg acaataagat
    33841 ccacggcacg gcaaatattt ataagaagaa gtaattctga accctatgta aacatgcccg
    33901 atgcgtaagt ttatcggtga tcatctattg aaatttatgc cggataaagc gttcgcgctg
    33961 cattcggcag ttcagctttt cagccgccgc cagaacgtcg tccggctgat gcctaaataa
    34021 ttcgccgctg ctgttttatc gccattaaat ttctccagtg cctgttgtgg tgtcagtaag
    34081 cgtggagcgg gagttttcgc cgactcgcgc gccagttccg gcagtagcag ctgcaaaaat
    34141 tgcggcgtta aatccggcgt cggttccaca cttaaaaata gcgccagtcg ctccatcata
    34201 ttgcgcagtt cacgaatatt gcccggccag tcgtagtgca ccagcacggt ttcgcttgcc
    34261 tgtaatccca ggcgtaatgc ggcagaaaac ggggcggaga gcgccgccag agacactttc
    34321 aaaaagcttt ccgccagcgg cagaatatcc gccacccgct cacgcagtgg tggcagttgc
    34381 aggcgtaaaa tgctcagccg ataaaacagg tcacggcgaa actgcccttg ccgcatatct
    34441 tcttccagct tgcagtgagt ggcgctaatg acccgcacat ccaccggaac aggctgatgc
    34501 ccgccgacgc gggtaacctc tttttcttcc agcacccgca gcagccgggt ctgcaaaggt
    34561 agcggcattt cgccaatctc atccaaaaac agcgtgccac cgtgggcgat ttcaaacagc
    34621 ccggcgcgac ctccgcgtcg cgagccggta aacgcccctt cctcatagcc aaacagctcc
    34681 gcttccagca gcgattcggc aatcgccccg cagttgacgg caacaaacgg atgcgacttt
    34741 ttgccctgtc gcgcatcgtg gcgggcaaag tattcccgat gaattgcctg ggctgccagt
    34801 tctttgcccg tccccgtttc cccctcaatc aacaccgccg cactggagcg ggcatacagc
    34861 aaaatagtct gccgcacctg ttccatctgt ggtgattgac cgagcatatc gcccagcacg
    34921 taacgagtac gcagggcgtt gcgagtggca tcgtgagtgt tatggcgtaa cgacatgcgc
    34981 gtcatatcca gcgcatcgct gaacgcctgg cgcacggtgg cggcggaata gataaaaatt
    35041 ccggtcattc cggcttcttc tgccagatcg gtaatcagcc ccgcgccgac caccgcttcg
    35101 gtgccgttag cttttagctc gttaatctgc ccgcgtgcgt cttcttcggt aatgtagcta
    35161 cgttgatcga ggcgcaaatt aaaggttttt tgaaacgcca ccagtgccgg aatagtttcc
    35221 tgataagtga caacgccgat agaagaggtg agttttccgg cttttgccag tgcctgtaac
    35281 acatcgtagc cgctcggttt aatcaaaata actggcactg acaggcggct tttcaggtac
    35341 gcgccgttag atccagccgc gatgatggcg tcacagcgtt cgtttgccag tttcttgtgg
    35401 atgtaggtca ccgctttttc aaagccaagc tggatagggg taatgttcgc caggtgatca
    35461 aactcgaggc tgatatcgcg aaacagctcg aacaggcgcg ttacagatac cgtccagata
    35521 accggtttgt cgtcattaag ccgtggtgga tgtgccatag cgcaccgcaa agttaagaaa
    35581 ccgaatattg ggtttagtct tgtttcataa ttgttgcaat gaaacgcggt gaaacattgc
    35641 ctgaaacgtt aactgaaacg catatttgcg gattagttca tgactttatc tctaacaaat
    35701 tgaaattaaa catttaattt tattaaggca attgtggcac accccttgct ttgtctttat
    35761 caacgcaaat aacaagttga taacaaagga tgggctatgt ctctacactc tccaggtaaa
    35821 gcgtttcgcg ctgcactgac taaagaaaat ccattgcaga ttgttggcac catcaacgct
    35881 aatcatgcgc tgttggcgca gcgtgccgga tatcaggcaa tttatctttc tggcggtggc
    35941 gtggcggcag gttcgctggg gctgcccgat ctcggtattt ctactcttga tgacgtgctg
    36001 accgatattc gccgtatcac cgacgtttgt tcgctgccgc tgctggtgga tgcggatatc
    36061 ggttttggtt cttcggcctt taacgtggcg cgcaccgtga aatcgatgat taaagccggt
    36121 gcggcaggat tgcatattga agatcaggtt ggtgcgaaac gctgcggtca tcgtccgaat
    36181 aaagcgatcg tctcgaaaga agagatggtg gatcggatcc gcgcggcggt ggatgcgaaa
    36241 accgatcctg attttgtgat catggcgcgc accgatgctc tggcggtaga ggggctggat
    36301 gcggcgatcg agcgtgcgca ggcctatgtt gaagcgggtg ccgagatgtt gttcccggag
    36361 gcgattaccg aactcgccat gtaccgccag tttgccgatg cggtgcaggt gccaatcctc
    36421 gccaacatta ccgaatttgg tgccacgccg ctgtttacca tcgacgaatt acgcagcgcc
    36481 catgtcgcaa tggcgctgta cccgctttca gcgttccgcg ccatgaaccg cgccgctgag
    36541 catgtctaca acgtcctgcg tcaggaaggc acgcagaaaa gcgtcatcga taccatgcaa
    36601 acccgcaacg agctgtacga aagcatcaac tactaccagt acgaagcgaa gctcgacgac
    36661 ctgtttgccc gtagccaggt gaaataaaac acaacgaaaa atatctcgac cctacaaatg
    36721 acgaggacaa catgagcgac acaacgatcc tgcaaaacag tacccatgtc attaaaccga
    36781 aaaaatctgt ggcactttct ggcgttccgg cgggcaatac ggcgctctgc accgtgggta
    36841 aaagtggcaa tgacctgcat taccgtggct acgatattct tgatctggcg gaacattgcg
    36901 aatttgaaga agtggcgcac ctgctgatcc acggcaaact gccgacccgt gacgaactcg
    36961 ccgcttacaa aacgaaactg aaagccctgc gcggtttacc ggctaacgtg cgtaccgtgc
    37021 tggaagcctt accggcggcg tcgtacccga tggatgtcat gcgcaccggc gtttccgcgc
    37081 tcggctgcac actgccagaa aaagaggggc acactgtctc tggcgcgcgg gatattgccg
    37141 acaaactgct ggcatcactt agctcgattc tcctttattg gtatcactac agtcacaacg
    37201 gcgaacgcat ccagccggaa accgatgacg actccatcgg tggtcacttc ctgcatctgc
    37261 tgcacggcga aaagccatcg caaagctggg aaaaggcgat gcatatttcg ctagtgctgt
    37321 acgccgaaca cgagtttaac gcctccacct ttaccagtcg ggtgattgcg ggcactggct
    37381 ctgatatgta ttccgcgatt attggcgcga ttggcgcact gcgcgggccg aaacacggcg
    37441 gagcgaatga agtgtcgctg gagatccagc aacgctacga aacgccggac gaagccgaag
    37501 ccgatatccg caagcgggtg gaaaacaaag aagtggtcat tggttttggt catccggttt
    37561 ataccatcgc cgacccgcgc caccaggtga tcaaacgtgt ggcgaagcag ctctcgcagg
    37621 aaggcggctc gttgaagatg tacaacatcg ccaatcgcct ggaaacggtg atgtgggaga
    37681 gcaaaaagat gttccccaat ctcgactggt tctctgctgt ttcctacaac atgatgggcg
    37741 ttcccaccga gatgttcaca ccactgtttg tcatcgcccg tgtcaccggc tgggcggcgc
    37801 acatcattga acaacgtcag gacaacaaaa ttatccgtcc ttccgccaat tatgttggac
    37861 cggaagaccg ccagtttgtc gcgctggata agcgccagta aacctctacg aataacaata
    37921 aggaaacata cccaatgtca gctcaaatca acaacatccg cccggaattt gatcgtgaaa
    37981 tcgttgatat cgtcgattac gtgatgaact acgaaatcag ctctaaagtg gcctacgaca
    38041 ccgcacatta ctgcctgctc gacacgctcg gctgcggtct ggaagctctc gaatacccgg
    38101 cctgtaaaaa actgctgggg ccaattgttc ctggcaccgt cgtacccaac ggcgtgcgcg
    38161 tccccggaac tcagtttcag ctcgaccctg tccaggcggc atttaacatt ggcgcgatga
    38221 tccgctggct cgatttcaac gatacctggc tggcggcgga gtgggggcat ccttccgaca
    38281 acctcggcgg cattctggca acggcagact ggctttcgcg caacgctgtc gccagcggca
    38341 aagcgccgtt gaccatgaaa caggtgctga ccgcaatgat caaagcccat gaaattcagg
    38401 gctgcatcgc gctggaaaac tcctttaacc gcgtcggcct cgaccacgtt ctgttagtga
    38461 aagtggcttc caccgccgtg gtcgccgaaa tgctcggcct gacccgcgag gaaattctca
    38521 acgccgtttc gctggcgtgg gtggacggtc agtcgctgcg cacctatcgc catgcgccga
    38581 acaccggcac gcgtaaatcc tgggcggcgg gcgatgccac ttcccgcgcg gtacgtctgg
    38641 cactgatggc gaaaacgggc gaaatgggtt acccgtcagc cctgaccgcg ccggtatggg
    38701 gcttctacga cgtctccttt aaaggtgaat cgttccgctt ccagcgtccg tacggttctt
    38761 acgtcatgga gaatgtgctg ttcaaaatct ccttcccggc ggagttccac tcccagacgg
    38821 cagttgaagc ggcgatgacg ctctatgaac agatgcaggc agcaggcaag acggcggcgg
    38881 atatcgaaaa agtgaccatt cgcacccacg aagcctgtat tcgcatcatc gacaaaaagg
    38941 ggccgctcaa taacccggct gaccgcgacc actgcattca gtacatggtg gcgatcccgc
    39001 tgttattcgg gcgcttaacg gcggcagatt acgaggacaa cgttgcgcaa gataaacgca
    39061 tcgacgccct gcgcgagaag atcaattgct ttgaagatcc ggcatttacc gctgactacc
    39121 acgacccgga aaaacgcgcc atcgccaatg ccattaccct tgagttcacc gacggcacac
    39181 gatttgaaga agtggtggtg gagtacccca ttggtcatgc tcgccgccgt caggatggta
    39241 ttccgaaact ggtcgataaa ttcaaaatca atctcgcgcg ccagttcccg actcgccaac
    39301 agcagcgcat tctggaggtt tctctcgaca gaactcgcct ggaacagatg ccggtcaatg
    39361 agtatctcga cctgtacgtc atttaagtaa actgcggtaa ggcgtaagtt caacaggaga
    39421 gcattatgtc ttttagcgaa ttttatcagc gttcgattaa cgaaccggag cagttctggg
    39481 ccgagcaggc ccggcgtatt gactggcaga cgccctttac gcaaacgctc gaccacagca
    39541 atccgccgtt tgcccgttgg ttttgtgaag gccgaaccaa tttgtgccac aacgctatcg
    39601 accgctggct ggagaaacag ccagaggcac tggcattgat tgccgtctct tcggaaacag
    39661 aagaagagcg cacctttacc tttcgccagc tgcatgacga agtgaacgcg gtggcctcaa
    39721 tgttgcgctc actgggcgtg cagcgcggcg atcgggtgct ggtgtatatg ccgatgattg
    39781 ccgaagcgca tattactctg ctggcctgcg cgcgcattgg tgctattcat tcggtggtat
    39841 ttggtggttt tgcctcgcac agcgtggcgg cgcgaattga tgacgctaaa ccggtgctga
    39901 ttgtctcggc tgatgccgga gcgcgcggtg gcaaaatcat tccctataaa aaattgctcg
    39961 acgatgcgat aagtcaggcg cagcaccagc cacgccatgt tttgctggtg gatcgcgggc
    40021 tggcgaaaat ggcgcgcgtc agcgggcggg atgtcgattt cgcgtcgttg cgccatcaac
    40081 acatcggcgc gcgggtaccg gtggcgtggc tggaatccaa cgaaacctcc tgcattctct
    40141 acacttccgg cacgaccggc aaacctaaag gcgtgcagcg tgacgtcggc ggatatgcgg
    40201 tggcgctggc gacctcgatg gacaccattt ttggcggcaa agcgggcagc gtgttctttt
    40261 gcgcatcgga tatcggctgg gtggtggggc attcgtatat cgtttacgcg ccgctgctgg
    40321 cggggatggc gactatcgtt tacgaaggat tgccgacctg gccggactgc ggcgtgtggt
    40381 ggaaaatcgt cgagaaatat caggttagcc ggatgttctc agcgccgacc gccattcgcg
    40441 tgctgaaaaa attccctacc gctgaaattc gcaaacacga tctctcgtcg ctggaagtgc
    40501 tctatctggc tggagaaccg ctggacgagc cgaccgccag ttgggtgagc aatacgctgg
    40561 atgtgccggt catcgacaac tactggcaga ccgaatccgg ctggccgatt atggcgattg
    40621 ctcgcggtct ggacgacagg ccgacgcgtc tgggaagccc cggcgtgccg atgtatggct
    40681 ataacgtgca gttgcttaat gaagtcaccg gcgaaccgtg tggcgtcaac gagaaaggga
    40741 tgctggtggt ggaagggccg ctgccgccgg gctgtattca gaccatctgg ggcgacgacg
    40801 accgctttgt gaagacttac tggtcgctgt tttcccgccc ggtgtacgcc acctttgact
    40861 ggggcatccg tgacgctgac ggttatcact ttattctcgg gcgcactgac gatgtaatta
    40921 acgttgccgg gcatcgtctg ggcacgcgcg agattgaaga gagtatctcc agccatccgg
    40981 gcgttgccga agtggcggtg gttggggtga aagatgcgct gaaagggcag gtggcggtgg
    41041 cgtttgtcat tccgaaagag agcgatagtc tggaagatcg tgatgtggcg cattcgcaag
    41101 agaaggcgat tatggcgctg gtggacagcc agattggcaa ctttggtcgc ccggcgcacg
    41161 tctggtttgt ctcgcaattg ccgaaaacgc gatccggaaa aatgctgcgc cgcacgatcc
    41221 aggcaatttg cgaaggacgc gatcctgggg atctgacgac cattgatgat cctgcgtcat
    41281 tggatcagat ccgccaggcg atggaagagt aaggtattgt cggatgcgtc gcgtggcgca
    41341 tccgacacca tgcgccgatg cctgatgcga cgcttaccgc gtcttattat gcctacaggc
    41401 ctgaaccgta ggtcggataa ggcgctcgcg tcgcatccga caccccgcgc cgatgcctga
    41461 tgcgacgctg acgcgtctta tcagtatcta cccactaccc actgttttta caccgataat
    41521 ttttccccca cctttttgca ctcattcata taaaaaatat atttccccac gaaaacgatt
    41581 gctttttatc ttcagatgaa tagaatacgg cggatttttt gggtttcaaa cagcaaaaag
    41641 ggggaatttc gtgtcgcaag ataacaactt tagccagggg ccagtcccgc agtcggcgcg
    41701 gaaaggggta ttggcattga cgttcgtcat gctgggatta accttctttt ccgccagtat
    41761 gtggaccggc ggcactctcg gaaccggtct tagctatcat gatttcttcc tcgcagttct
    41821 catcggtaat cttctcctcg gtatttacac ttcatttctc ggttacattg gcgcaaaaac
    41881 cggcctgacc actcatcttc ttgctcgctt ctcgtttggt gttaaaggct catggctgcc
    41941 ttcactgcta ctgggcggaa ctcaggttgg ctggtttggc gtcggtgtgg cgatgtttgc
    42001 cattccggtg ggtaaggcaa ccgggctgga tattaatttg ctgattgccg tttccggttt
    42061 actgatgacc gtcaccgtct tttttggcat ttcggcgctg acggttcttt cggtgattgc
    42121 ggttccggct atcgcctgcc tgggcggtta ttccgtgtgg ctggctgtta acggcatggg
    42181 cggcctggac gcattaaaag cggtcgttcc cgcacaaccg ttagatttca atgtcgcgct
    42241 ggcgctggtt gtggggtcat ttatcagtgc gggtacgctc accgctgact ttgtccggtt
    42301 tggtcgcaat gccaaactgg cggtgctggt ggcgatggtg gcctttttcc tcggcaactc
    42361 gttgatgttt attttcggtg cagcgggcgc tgcggcactg ggcatggcgg atatctctga
    42421 tgtgatgatt gctcagggcc tgctgctgcc tgcgattgtg gtgctggggc tgaatatctg
    42481 gaccaccaac gataacgcac tctatgcgtc gggtttaggt ttcgccaaca ttaccggtat
    42541 gtcgagcaaa accctttcgg taatcaacgg tattatcggt acggtctgcg cattatggct
    42601 gtataacaat tttgtcggct ggttgacctt cctttcggca gctattcctc cagtaggggg
    42661 cgtgatcatc gccgactatc tgatgaaccg tcgccgctat gagcactttg cgaccacgcg
    42721 tatgatgagt gtcaattggg tggcgattct ggcggtcgcg ctggggattg ccgcaggcca
    42781 ctggttaccg ggaattgttc cggtcaacgc ggtattaggt ggcgcgctga gctatctgat
    42841 ccttaacccg attttgaatc gtaaaacgac agcagcaatg acgcatgtgg aggttaacag
    42901 tgtcgaataa cgctttacaa acaattatta acgcccggtt accaggcaaa gaggggctgt
    42961 ggcagattca tctgcaggac ggaaaaatca gcgccattga tgcgcaatcc ggcgtgatgc
    43021 ccataactga aaacagcctg gatgccgaac aaggtttagt tataccgccg tttgtggagc
    43081 cgcatattca cctggacacc acgcaaaccg ccggacaacc gaactggaat cagtccggca
    43141 cgctgtttga aggcattgaa cgctgggccg agcgcaaagc gttattaacc catgacgatg
    43201 tgaaacaacg cgcctggcaa acgctgaaat ggcagattgc caacggcatt cagcatgtgc
    43261 gtacccatgt cgatgtttcg gatgcaacgc taactgcgct gaaagcaatg ctggaagtga
    43321 agcaggaagt cgcgccgtgg attgatctgc aaattgtcgc cttccctcag gaagggattt
    43381 tgtcgtatcc caacggtgaa gcgttgctgg aagaggcgtt acgcttaggg gcagatgtag
    43441 tgggggcgat tccgcatttt gaatttaccc gtgaatacgg cgtggagtcg ctgcataaaa
    43501 ccttcgccct ggcgcaaaaa tacgaccgtc tcatcgacgt tcactgtgat gagatcgatg
    43561 acgagcagtc gcgctttgtc gaaaccgttg ctgccctggc gcaccgtgaa ggcatgggcg
    43621 cgcgagtcac cgccagccac accacggcaa tgcactccta taacggggcg tatacctcac
    43681 gcctgttccg cttgctgaaa atgtccggta ttaactttgt cgccaacccg ctggtcaata
    43741 ttcatctgca aggacgtttc gatacgtatc caaaacgtcg cggcatcacg cgcgttaaag
    43801 agatgctgga gtccggcatt aacgtctgct ttggtcacga tgatgtcttc gatccgtggt
    43861 atccgctggg aacggcgaat atgctgcaag tgctgcatat ggggctgcat gtttgccagt
    43921 tgatgggcta cgggcagatt aacgatggcc tgaatttaat cacccaccac agtgccagaa
    43981 cattgaattt gcaggattac ggcattgccg ccggaaacag cgcaaacctg attatcctgc
    44041 cggctgaaaa tggatttgat gcgctgcgcc gtcaggttcc ggtacgttat tcggtacgtg
    44101 gcggcaaggt gattgccagt acacaaccgg cacaaaccac cgtatatctg gagcagccag
    44161 aagccatcga ttacaaacgg taatttcctc tgtgttgaag agccactgac ctgtgtcagt
    44221 ggctaccgtg attcattctc gccaacatcc gcacattcct ccaacgccat gtgcaaaaat
    44281 gccttcgcgg cggctgtctg ccagctattt ttccgccgca acaaaaccgc cgttctctcc
    44341 agtagtggcg gggcaagaga aatcgcttta agcccgtcat gttgtgtggc aatcgctgct
    44401 ggtaacaatg tggaaaggga agtgcggcga atcagctcca gaaccgcgct aattgagttc
    44461 gcctcaatga ccacctgtgg atgtagcccc gctttctcgc agtagtggtc aatttgctct
    44521 ctggtggcaa attccgcgct gagcaggacc agtttttcat catgcaagcg actcaacgcc
    44581 acctgttcac aggcagccag cggatgatgt tgcgccacga ctaacgctaa actttctgtc
    44641 agtaaaggaa ttgcctccag ctccggcgaa tgcacaggcg cgaaggcaat cccaacgtcc
    44701 agctcgtcgc ggcaaagcag atcttcgatt ttctcctgcg acatttcctg tagctggagc
    44761 gtgatgccgg ggtagcgcgc atagaaatcc gccattaaag ggccgataaa gtagcttgta
    44821 aaggtggggg tgacggcgat acgcagcgat cctcgcgtca gatcggcaac atcatgaatc
    44881 gcccgtttac ccgctcccag ttcctgtaac gcccggctgg cgtactgtcg ccagacttct
    44941 cctgcatcgg tgagacgaat cgttcgcccg ctacggtcaa acagcggcac gcctaaactc
    45001 tcctctaact ggcgaatctg ctgggaaagc gcaggctgag agacgtgcaa cgcactggcg
    45061 gcacgggtga agctgccatg ttcagccacg gcaagaaaat aattgatatg tcgagagagc
    45121 attcgccaac ctataagtaa atccaataga actcatcata aatgagactt ttaccttatg
    45181 acaatcggcg agtagtctgc ctctcattcc agagacagac agaggttaac ggtgaaagag
    45241 attattgatg gattccttaa attccagcgc gaggcatttc cgaagcggga agccttgttt
    45301 aaacagctgg cgacacagca aagcccgcgc acacttttta tctcctgctc cgacagccgt
    45361 ctggtccctg agctggtgac gcaacgtgag cctggcgatc tgttcgttat tcgcaacgcg
    45421 ggcaatatcg tcccttccta cgggccggaa cccggtggcg tttctgcttc ggtggagtat
    45481 gccgtcgctg cgcttcgggt atctgacatt gtgatttgtg gtcattccaa ctgtggcgcg
    45541 atgaccgcca ttgccagctg tcagtgcatg gaccatatgc ctgccgtctc ccactggctg
    45601 cgttatgccg attcagcccg cgtcgttaat gaggcgcgcc cgcattccga tttaccgtca
    45661 aaagctgcgg cgatggtacg tgaaaacgtc attgctcagt tggctaattt gcaaactcat
    45721 ccatcggtgc gcctggcgct cgaagagggg cggatcgccc tgcacggctg ggtctacgac
    45781 attgaaagcg gcagcatcgc agcttttgac ggcgcaaccc gccagtttgt gccactggcc
    45841 gctaatcctc gcgtttgtgc cataccgcta cgccaaccga ccgcagcgta accttatttt
    45901 taaaccatca ggagatccac catgattcag tcacaaatta accgcaatat tcgtcttgat
    45961 cttgccgatg ccattttgct cagcaaagct aaaaaagatc tctcatttgc cgagattgcc
    46021 gacggtaccg gtctggcaga agccttcgta accgcggctt tgctggggca gcaagcgctt
    46081 cctgccgacg ccgcccgcct ggtcggggcg aagctggatc tcgacgaaga cgccattcta
    46141 ctgttgcaga tgattccact gcgtggctgc attgatgacc gtattccaac cgacccaacg
    46201 atgtatcgtt tctatgaaat gttgcaggtg tacggcacaa ccctgaaagc gttggttcat
    46261 gagaagtttg gcgatggcat tatcagcgcg attaacttca aactcgacgt taagaaagtg
    46321 gcggacccgg aaggtggcga acgtgcggtc attaccttag atggtaaata tctgccgacc
    46381 aagccgttct gacagccatg cgcaatcatc aaaagacgtt cacgatgctg ctggttctgg
    46441 tgctgattgg tcttaatatg cgaccactgc tcacctccgt cgggccactg ctaccgcaat
    46501 tgcgccaggc gagcggaatg agctttagcg tggctgccct gttgaccgct cttccggtgg
    46561 tcaccatggg cgggctggcg ctggccggaa gctggcttca tcagcatgtc agcgaacgtc
    46621 gcagtgtcgc catcagtctg ttgctgattg ccgtcggtgc attgatgcgt gagctttacc
    46681 cccaaagtgt gctgctgctt agcagcgcac tgcttggtgg ggtggggatc ggtatcattc
    46741 aggcggtgat gccttcagtg attaaacggc ggtttcagca acgcacgcca ctggtgatgg
    46801 ggctgtggtc tgcggctctg atgggcggcg gtggacttgg tgccgccata actccctggt
    46861 tagttcaaca tagcgaaacc tggtatcaaa cactcgcctg gtgggcgctg cctgccgttg
    46921 ttgcgctctt tgcctggtgg tggcaaagcg cccgcgaggt cgcctcttcc cacaagacaa
    46981 caaccactcc ggttcgcgtg gtattcactc cccgcgcgtg gacgctgggt gtttacttcg
    47041 gtctgattaa cggcggttac gccagcctga ttgcctggtt acccgctttc tatattgaga
    47101 ttggtgccag cgcgcagtac agcggttcct tactggcatt gatgacgctt gggcaagccg
    47161 caggagcttt gctgatgcct gctatggctc gccatcagga tcggcgcaaa ctgttaatgc
    47221 tggcgctggt gttacaactg gtggggttct gcggctttat ctggttgccg ctgcaattgc
    47281 cggtatcgtg ggcgatggtg tgtgggttag gtctgggcgg cgcgtttccg ctctgtttgc
    47341 tgctggcgct cgatcactct gtgcaaccgg ctattgctgg caagctggtg gcgtttatgc
    47401 aggggatcgg ttttatcatc gccgggcttg ccccgtggtt ttctggcgtg ctgcgtagta
    47461 tcagcggcaa ttacctgatg gactgggcgt ttcatgcgct gtgcgtcgtt gggctgatga
    47521 tcataaccct gcgttttgca ccagcacgtt ttccgcagct gtgggtcaaa gaggtatgat
    47581 gcgacgcttg tttcgccaga tgatggagct ataaataatt gtcggatgtg cccagtggcg
    47641 catccgacat taattgctta agcgacttca ttcacctgac gacgcagcag ggaaagcggg
    47701 ccggggccgc taagcgtgaa cacggaaatt aaggtgaagc ccagcgccac cagacccagc
    47761 accagataag cgccctggaa accgatgctt tcatacatat tgcccgccag tacagacata
    47821 aaaatcatcg ccagttgctt aaagaagcag aaacagacca gataaatcgt cgctgaaaaa
    47881 cgcacttcaa actggctggt aatatattta aagcagccca ccagcaggaa cggtacttca
    47941 aacatatgca gcgttttcag aataaccact tccagcgctg aggtggcgaa cgatgagcca
    48001 ataatacgta cagacataat agtgccagcc agcagcaggg cgtttttccc accgatgcga
    48061 ttaatgatca gtggcgcaaa gaacataatc gaggcgttaa gtaattcgcc cattgtcgtt
    48121 acgtagccaa atacccgcgt accctgttca ccggtagcaa agaacgaagt aaagaaatta
    48181 gcaaactgtt ggtcaaaaac atcgtaggtg caggaaacgc caataacata cagtgacaaa
    48241 aaccacagtt ttggctgtct gaacagttcc agtgccagct taaggctaaa tgccgaatgg
    48301 ttggcaccta ccgcattggc aaccgtggcg gaagagggcg catccgtttt ggcgaaaaag
    48361 agtaaaacgg cgaggatgag tgcacagcca gaacccagcc agaaaacaaa ctgattattg
    48421 atggtgaaca tgatgccgac aatcgaggca cacaacgccc agccaacaca gccaaacatc
    48481 cgcgcgcgac caaattcgaa attactgcga cggctgactt tctcaataaa tgcctctact
    48541 gctggcgcac cggcgttaaa acaaaagcct agataaatac caccaacaat cgatcctact
    48601 aaaatgttgt attgtaacag tggcccgaag ataaaaataa agaacggcgc aaacatcact
    48661 aacatgccgg taataatcca cagcaggtat ttgcgcagcc cgagtttgtc agaaagcaga
    48721 ccaaacagcg gttggaataa tagcgagaac agagaaatag cggcaaaaat aatacccgta
    48781 tcacttttgc tgatatggtt gatgtcatgt agccaaatcg ggaaaaacgg gaagtaggct
    48841 cccatgataa aaaagtaaaa gaaaaagaat aaaccgaaca tccaaaagtt tgtgtttttt
    48901 aaatagtaca taatggattt ccttacgcga aatacgggca gacatggcct gcccggttat
    48961 tattattttt gacaccagac caactggtaa tggtagcgac cggcgctcag ctggaattcc
    49021 gccgacactg acgggctcca ggagtcgtcg ccaccaatcc ccatatggaa accgtcgata
    49081 ttcagccatg tgccttcttc cgcgtgcagc agatggcgat ggctggtttc catcagttgc
    49141 tgttgactgt agcggctgat gttgaactgg aagtcgccgc gccactggtg tgggccataa
    49201 ttcaattcgc gcgtcccgca gcgcagaccg ttttcgctcg ggaagacgta cggggtatac
    49261 atgtctgaca atggcagatc ccagcggtca aaacaggccg cagtaaggcg gtcgggatag
    49321 ttttcttgcg gccctaatcc gagccagttt acccgctctg cgacctgcgc cagctggcag
    49381 gtcaggccaa tccgtgccgg atgcggtgta tcgctcgcta cttcaacatc aacggtaatc
    49441 gccatttgac cactaccatc aatccggtag gttttccggc tgataaataa ggtttttccc
    49501 tgatgctgcc acgcgtgagc ggtcgtaatc agcaccgcgt cggcaagtgt atctgccgag
    49561 cactgcaaca acgctgcttc ggcctggtaa tggcccgccg ccttccagcg ttcgacccag
    49621 gcgttagggt caatgcgggt cgcttcactt acgccaatgt cgttatccag cggtgcacgg
    49681 gtgaactgat cgcgcagcgg cgtcagcagt tgtttttcat cgccaatcca catctgtgaa
    49741 agaaggcctg actgacggtt aaattgccaa cgcttattac ccagctcgat gcaaaaatcc
    49801 atttcgctgg tggtcagatg cgggatggcg tgggacgcgg cggggagtgt cacgctgagg
    49861 ttttccgcca gacgccactg ctgccaggcg ctgatgtgtc cggcttctga ccatgcggtc
    49921 gcgttcggtt gcactacgtg taccgtaagc cagagttgtc cggcgctctc cggctgcggt
    49981 agtccaggca gttcaatcaa ctgtttacct tgtggagcga catccagagg cacttcaccg
    50041 cttgccagcg gcttgccatc cagcgccacc atccagtgca ggagctcgtt atcgctatga
    50101 cggaacaggt attcgctggt cacttcgatg gtttgcccgg ataaacggaa ctggaaaaac
    50161 tgctgctggt attttgcttc cgtcagcgcc ggatgcggcg tgcggtcggc aaagaccaga
    50221 ccgttcatgc agaactggcg atcgttcggc gtatcgccaa aatcaccgcc gtaagccaac
    50281 cacgggttgc cgttttcatc atatttaatc agcgactgat ccacccagtc ccagacgaag
    50341 ccgccctgta aacggggata ctgacgaaac gcctgccagt atttagcgaa accgccaaga
    50401 ctgttaccca ttgcgtgggc atattcgcaa aggatcagtg ggcgcagttc tccaggcagc
    50461 gaaagccatt ttttgatgga ccatttcggc accgccggga agggttggtc ttcatccacg
    50521 cgcgcgtaca tcgggcaaat aatatcggtt gcgaaggtgt cggctccgcc gccttcatac
    50581 tgcaccgggc gggaaggatc gacagatttg atccagcgat acagcgcgtc gtgattagcg
    50641 ccgtggcctg actcattccc cagcgaccag atgatcacac tcgggtgatt acgatcgcgc
    50701 tgcaccattc gcgttacgcg ttcgctcatc gccggtagcc agcgcggatc atcggtcaga
    50761 cgattcattg gcaccatgcc gtgggtttca atattggctt catccaccac atacaggccg
    50821 tagcggtcgc acagcgtgta ccacagcgga tggttcggat aatgcgaaca gcgcacggcg
    50881 ttaaagttgt tctgcttcat cagcaggata tcctgcacca tcgtctgctc atccatgacc
    50941 tgaccatgca gaggatgatg ctcgtgacgg ttaacgcctc gaatcagcaa cggcttgccg
    51001 ttcagcagca gcagaccatt ttcaatccgc acctcgcgga aaccgacatc gcaggcttct
    51061 gcttcaatca gcgtgccgtc ggcggtgtgc agttcaacca ccgcacgata gagattcggg
    51121 atttcggcgc tccacagttt cgggttttcg acgttcagac gtagtgtgac gcgatcggca
    51181 taactaccac gctcatcgat aatttcaccg ccgaaaggcg cggtgccgct ggcgacctgc
    51241 gtttcaccct gccataaaga aactgttacc cgtaggtagt cacgcaactc gccgcacatc
    51301 tgaacttcag cctccagtac agcgcggctg aaatcatcat taaagcgagt ggcaacatgg
    51361 aaatcgctga tttgtgtagt cggtttatgc agcaacgaga cgtcacggaa aatgccgctc
    51421 atccgccaca tatcctgatc ttccagataa ctgccgtcac tccagcgcag caccatcacc
    51481 gcgaggcggt tttctccggc gcgtaaaaat gcgctcaggt caaattcaga cggcaaacga
    51541 ctgtcctgac cgtaaccgac ccagcgaccg ttgcaccaca gatgaaacgc cgagttaacg
    51601 ccatcaaaaa taattcgcgt ctggccttcc tgtagccagc tttcatcaac attaaatgtg
    51661 agcgagtaac aacccgtcgg attctccgtg ggaacaaacg gcggattgac cgtaatggga
    51721 taggtcacgt tggtgtagat gggcgcatcg taaccgtgca tctgccagtt tgaggggacg
    51781 acgacagtat cggcctcagg aagatcgcac tccagccagc tttccggcac cgcttctggt
    51841 gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
    51901 tcggtgcggg cttcttcgct attacgccag ctggcgaaag ggggatgtgc cgcaaggcga
    51961 ttaagttgtg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
    52021 tccgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caatttcaca
    52081 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    52141 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    52201 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
    52261 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
    52321 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
    52381 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
    52441 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
    52501 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
    52561 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
    52621 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
    52681 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
    52741 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg agtctggtca gagacatcaa
    52801 gaaataacgc cggaacatta gcgcaggctg cttccacagc agtggcatcc tggtcatcca
    52861 gcggatagtt aatgagcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
    52921 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
    52981 cagcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
    53041 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
    53101 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
    53161 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
    53221 cgtatagcgt tactggtttc acattcacca ccctgaattg actctcttcc gaacgctatc
    53281 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc aacgtaaatg cacgccgctt
    53341 cgccgtccgg ccaccagaat agcctgcgat tcaacccctt cttcgatctg ttttgctacc
    53401 cgttgtagcg ccggaagatg cttttccgct gcctgttcaa tggtcattgc gctcgccata
    53461 tacaccagat tcagacagcc aatcacccgt tgttcactgc gcagcggtac ggcgatagag
    53521 gcgatcttct cctcctgatc ccagccgcgg tagttctgtc cgtaaccctc tttgcgcgcg
    53581 cgcgccagaa tggcttccag ctttaacggt tcccgtgcca gttgatagtc atcaccgggg
    53641 cgggaggcta acatttcgat taattccttg cggtcttgtt ccgggcaaaa ggccagccag
    53701 gtcaggcccg aggcggtttt cagaagcggc aaacgtcgcc cgaccattgc ccggtgaaag
    53761 gataagcggc tgaaacggtg agtggtttcg cgtaccacca ttgcatcaac atccagcgtg
    53821 gacacatctg tcggccatac cacttcgcgc aacagatcgc ccagcagtgg ggccgccagt
    53881 gcagaaatcc actgttcgtc acgaaatcct tcgcttaatt gccgcacttt gatggtcagt
    53941 cgaaaactat catcggaggg gctacggcgg acatatccct cttcctgcag cgtctccagc
    54001 agtcgccgca cagtggtgcg atgcaggccg ctgagttccg ccagcagccc gacgctggca
    54061 ccgccatcaa gtttatttaa catatttaat aacattagac cgcgggttaa gccgcgcacg
    54121 gttttgtatt ccgtctgctc attgttctgc atattaattg acatttctat agttaaaaca
    54181 acgttgtgca cctggtgcac attcgggcat gttttgattg tagccgaaaa cacccttcct
    54241 atactgagcg cacaataaaa aatcatttac atgtttttaa caaaataagt tgcgccgtac
    54301 tgtgcgcgca acggcatttt gtccgagtcg tgaggtactg aaatggcaat acaacaccct
    54361 gacatccagc ctgctgttaa ccatagcgtt caggtggcga tcgcaggtgc cggtccggtc
    54421 gggctgatga tggcgaacta tctcggtcag atgggcattg acgtgctggt ggtggagaaa
    54481 ctcgataagt tgatcgacta cccgcgtgcg attggtattg atgacgaggc gctgcgcacc
    54541 atgcagtcgg ttggcctggt cgagaatgtt ctgccgcaca ctacgccgtg gcacgcgatg
    54601 cgttttctca ccccgaaagg ccgctgtttt gctgatattc agccaatgac cgatgaattt
    54661 ggctggccgc gccgtaacgc ctttattcag ccgcaggtcg atgcggtgat gctggaaggg
    54721 gtgtcgcgtt ttcctaatgt gcgctgcttg ttttcccgcg agctggaggc cttcagccag
    54781 caaaatgacg aagtgacctt gcacctgaaa acggcagaag ggcagcggga aatagtcaaa
    54841 gcccagtggc tggtagcctg tgacggtgga gcaagttttg tccgtcgcac tctgaatgtg
    54901 ccgtttgaag gtaaaactgc gccaaatcag tggattgtgg tagatatcgc caacgatccg
    54961 ttaagtacgc cgcatatcta tttgtgttgc gatccggtgc gcccgtatgt ttctgccgcg
    55021 ctgcctcatg cggtacgtcg ctttgaattt atggtgatgc cgggagaaac cgaagagcag
    55081 ctgcgtgagc cgcaaaatat gcgcaagctg ttaagcaaag tgctgcctaa tccggacaat
    55141 gttgaattga ttcgccagcg tgtctacacc cataacgcgc gactggcgca acgtttccgt
    55201 attgatcgcg tactgctggc gggagatgcc gcgcacatca tgccggtgtg gcaggggcag
    55261 ggctataaca gtggtatgcg cgacgccttt aacctcgcct ggaaactggc gttggttatc
    55321 caggggaaag cccgcgatgt gctgctcgat acctatcaac aagaacgtcg cgatcacgcc
    55381 aaagcgatga ttgacctgtc cgtgacggcg ggcaacgtgc tggctccgcc gaaacgctgg
    55441 cagggtacgt tacgtgacgg cgtttcctgg ctactgaatt atctgccgcc agtaaaacgc
    55501 tacttcctcg aaatgcgctt caagccgatg ccgcaatatt acggcggtgc gctggtacgt
    55561 gagggcgaag cgaagcactc tccggtcggc aagatgttta ttcagccgaa agtcacgctg
    55621 gaaaacggcg acgtgacgct gctcgataac gcgattggcg cgaacttcgc ggtaattggc
    55681 tggggatgca atccactgtg ggggatgagc gacgagcaaa tccagcagtg gcgcgcgttg
    55741 ggcacacgct tcattcaggt ggtgccggaa gtgcaaattc ataccgcaca ggataaccac
    55801 gacggcgtgc tacgcgtggg cgatacgcaa ggtcgtctgc gtagctggtt cgcgcaacac
    55861 aatgcttcgc tggtggtgat gcgcccggat cgctttgttg ccgccaccgc cattccgcaa
    55921 accctgggca atacgctgaa taaactggcg tcggtgatga cgctgacccg ccctgatgcc
    55981 gacgtttctg tcgaaaaggt agcctgatat gcacgcttat cttcactgtc tttcccactc
    56041 gccgttggtg gggtatgtcg acccggcgca agaggtactc gatgaggtca atggcgtgat
    56101 tgccagcgcc cgcgagcgta ttgcggcttt ctcccctgaa ctggtggtgc tgtttgcgcc
    56161 agatcactac aacggctttt tctatgacgt gatgccaccg ttctgtttag gcgttggagc
    56221 gacggcaatt ggtgatttcg gcagtgcggc aggagagctg cccgtgcctg tggagctggc
    56281 ggaggcctgt gcgcatgctg tcatgaagag cgggatcgat cttgccgttt cttactgtat
    56341 gcaggtggac cacgggttcg cccagccgct ggagttcctg ctcggtgggc tggataaggt
    56401 gccagttctg cctgtgttca tcaacggtgt cgccacgccg ctgcccggtt tccagcgtac
    56461 ccgcatgttg ggtgaagcca ttggacgttt caccagcact ctcaataaac gcgtgctgtt
    56521 cctgggttcc ggtgggcttt cccatcagcc gccggtgccc gaactggcga aagccgatgc
    56581 ccatatgcgc gaccgtctgt tggggagcgg gaaagattta cccgccagtg agcgcgaatt
    56641 gcgtcagcaa cgggtgatta gcgccgctga gaagtttgtt gaggatcaga gaactctgca
    56701 tccgctcaac ccgatttggg ataaccagtt catgactttg ctggagcagg gacgcataca
    56761 ggaactggat gccgtcagta acgaagagct ttccgccatt gccggaaagt cgacacatga
    56821 aatcaaaacc tgggtcgccg cttttgccgc tatttctaca tttggcaact ggcgtagcga
    56881 agggcgttat taccgcccaa tcccggagtg gattgccgga tttggctcgt taagcgccag
    56941 aacagagaac tgaatatgca ggagaagatg atgagttatc agccacaaac cgaagccgcc
    57001 accagccgtt ttctgaatgt agaagaagcg ggtaaaacgc tgcgcatcca ttttaatgac
    57061 tgcggacaag gcgacgaaac cgttgtcctg ctgcatggtt ccggcccggg tgctactggc
    57121 tgggcgaact tcagccgcaa tatcgatccg ctggtagagg cgggctatcg ggtaatcctg
    57181 ctggattgtc cgggttgggg caaaagcgat tcgatcgtta atagtggttc acgatcggat
    57241 cttaatgcac gaatcctgaa aagcgtggtg gatcaactgg atatcgccaa aatccacctg
    57301 ctgggcaact cgatgggcgg ccatagttct gtggcgttca cccttaactg gccggagcgc
    57361 gtcggcaaac tggtgctgat gggcggcggt acgggcggca tgagtttgtt tacgccgatg
    57421 ccaaccgaag gtattaagcg actgaatcag ctttatcgtc agccgactat cgaaaacctg
    57481 aagctgatga tggatatctt cgttttcgat accagcgatt tgaccgacgc cctgtttgaa
    57541 gcgcgcctga ataatatgct gtcgcgccgc gatcaccagg aaaacttcgt taagagcctg
    57601 gaagctaatc cgaaacagtt cccggatttt ggcccacgtc tggcggaaat caaagcgcaa
    57661 accctgattg tctgggggcg caacgaccgc tttgtgccga tggatgcggg tctgcgtctg
    57721 ctgtccggca ttgccggttc tgaactgcat atcttccgcg actgtggtca ctgggcgcag
    57781 tgggaacatg ccgacgcttt caatcaattg gtgctgaatt tcctcgcacg cccttaagga
    57841 atggtcatga cgaagcatac tcttgagcaa ctggcggcgg atttacgccg cgccgcagag
    57901 cagggcgaag cgattgcgcc gctgcgcgat ctgattggta tcgataacgc tgaagcggcc
    57961 tacgccattc agcacataaa tgtgcaatat gacgttgtgc aggggcgtcg cgtggtaggg
    58021 cgtaaagtgg gcctgacgca tccgaaagtg caacaacaac tgggcgttga tcaaccggat
    58081 tttgggacgt tatttgccga catgtgttat ggcgataacg aaatcattcc tttttcccgt
    58141 gttctgcaac cccgcattga agcggagatc gcactggtgt tgaaccgcga tttacccgca
    58201 actgatatca ccttcgacga actgtataac gccattgaat gggtacttcc ggcgctggaa
    58261 gtggtgggga gccgcattcg cgactggtcg attcagtttg tcgataccgt ggcagataac
    58321 gcttcctgcg gggtgtatgt catcggcggt ccggcgcaac gtccggcggg gttagacctg
    58381 aaaaactgcg ccatgaagat gacgcgtaat aacgaagagg tttccagcgg gcgcggcagc
    58441 gaatgcctgg gacatccgct taatgccgcc gtctggctgg cacgcaaaat ggccagtctg
    58501 ggtgaaccgc tgcgcgccgg agatatcatt cttaccgggg cattaggccc gatggtggcg
    58561 gtgaatgcgg gcgatcgttt tgaagcccat attgaaggca taggttcagt tgctgcgaca
    58621 ttttcaagcg cagccccaaa aggaagtctg tcatgagtaa gcgtaaagtc gccattatcg
    58681 gttctggcaa cattggcacc gatctgatga ttaaaatttt gcgtcacggt cagcatctgg
    58741 agatggcggt gatggtcggc attgatcctc agtccgacgg tctggcgagt gccagacgta
    58801 tgggcgtcgc caccacccat gaaggggtga tcgggctgat gaacatgcct gaatttgctg
    58861 atatcgacat tgtatttgat gcgaccagcg cgggcgcgca tgtgaaaaac gatgccgctt
    58921 tacgcgaagc gaaaccggat attcgcttaa ttgacctgac gcctgctgcc atcggccctt
    58981 actgcgtgcc ggtggttaac ctcgaggcga acgtcgatca actgaacgtc aacatggtca
    59041 cctgcggcgg ccaggccacc attccaatgg tggcggcagt ttcacgcgtg gcgcgtgttc
    59101 attacgccga aattatcgct tctatcgcca gtaaatctgc cggacctggc acgcgtgcca
    59161 atatcgatga atttacggaa accacttccc gagccattga agtggtgggc ggcgcggcaa
    59221 aagggaaggc gattattgtg cttaacccag cagagccacc gttgatgatg cgtgacacgg
    59281 tgtatgtatt gagcgacgaa gcttcacaag atgatatcga agcctcaatc aatgaaatgg
    59341 ctgaggcggt gcaggcttac gtaccgggtt atcgcctgaa acagcgcgtg cagtttgaag
    59401 ttatcccgca ggataaaccg gtcaatttac cgggcgtggg gcaattctcc ggactgaaaa
    59461 cagcggtctg gctggaagtc gaaggcgcag cgcattatct gcctgcctat gcgggcaacc
    59521 tcgacattat gacttccagt gcgctggcga cagcggaaaa aatggcccag tcactggcgc
    59581 gcaaggcagg agaagcggca tgaacggtaa aaaactttat atctcggacg tcacgttgcg
    59641 tgacggtatg cacgccattc gtcatcagta ttcgctggaa aacgttcgcc agattgccaa
    59701 agcactggac gatgcccgcg tggattcgat tgaagtggcc catggcgacg gtttgcaggg
    59761 ttccagcttt aactatggtt tcggcgcaca tagcgacctt gaatggattg aagcagcggc
    59821 ggatgtggtg aagcacgcca aaatcgcgac gttgttgctg ccaggaatcg gcactattca
    59881 cgatctgaaa aatgcctggc aggctggcgc gcgggtggtt cgtgtggcaa cgcactgtac
    59941 cgaagctgat gtttccgccc agcatattca gtatgcccgc gagctcggaa tggacaccgt
    60001 tggttttctg atgatgagcc atatgaccac gccggagaat ctcgcaaagc aggcaaagct
    60061 gatggaaggc tacggtgcga cctgtattta tgtggtggat tctggcggtg cgatgaacat
    60121 gagcgatatc cgtgaccgtt tccgcgccct gaaagcagag ctgaaaccag aaacgcaaac
    60181 tggcatgcac gctcaccata acctgagtct tggcgtggcg aactctatcg cggcggtgga
    60241 agagggctgc gaccgaatcg acgccagcct cgcgggaatg ggcgcgggcg caggtaacgc
    60301 gccgctggaa gtatttattg ccgccgcgga taaactgggc tggcagcatg ggaccgatct
    60361 ctatgcgtta atggatgccg ctgacgacct ggtgcgtccg ttgcaggatc gaccggtacg
    60421 agtcgatcgc gaaacgctgg cgctgggata cgctggtgtt tactcgagct tcctgcgtca
    60481 ctgtgaaacg gcggcggcgc gttatggctt aagtgcggtg gatattctcg ttgagctggg
    60541 caaacgccgg atggttggcg gccaggagga tatgatcgtt gacgtggcgc tggatctgcg
    60601 caacaacaaa taatgatgac tgccgagaac gtgcattttg tatgcccatc ccagcacgac
    60661 tgccggatgc ggcgtaaaca ccttatccgg cctacgggtg gcgtgagaat ttgtaggcct
    60721 gataagacgc gttagcgtcg catcaggcat ctgcacacga ctgccggatg cgataaacgt
    60781 cttgtccggc ctacatttcg cccgtaggca gtcattaaat agttctgatt acgcggctgg
    60841 caatgtatca gtcgcgattc acctgcactc gcaacgaggt tcatcttatg tcgactcgta
    60901 ccccttcatc atcttcatcc cgcctgatgc tgactatcgg gctttgtttt ttggtcgctc
    60961 tgatggaagg gctggatctt caggcggctg gcattgcggc gggtggcatc gcccaggctt
    61021 tcgcactcga taaaatgcaa atgggctgga tattcagcgc cggaatactc ggtttgctac
    61081 ccggcgcgtt ggttggcgga atgctggcgg accgttatgg tcgcaagcgc attttgattg
    61141 gctcagttgc gctgtttggt ttgttctcgc tggcaacggc gattgcctgg gatttcccct
    61201 cactggtctt tgcgcggctg atgaccggtg tcgggctggg ggcggcgttg ccgaatctta
    61261 tcgccctgac gtctgaagcc gcgggtccac gttttcgtgg gacagcagtg agcctgatgt
    61321 attgcggtgt tcccattggc gcggcgctgg cggcgacact gggtttcgcg ggggcaaact
    61381 tagcatggca aacggtgttt tgggtaggtg gtgtggtgcc gttgattctg gtgccgctat
    61441 taatgcgctg gctgccggag tcggcggttt tcgctggcga taaacaggct gcgccaccac
    61501 tgcgtgcctt atttgcgcca gaaacggcaa ccgcgacgct gctgctgtgg ttgtgttatt
    61561 tcttcactct gctggtggtc tacatgttga tcaactggct accgctgctt ttggtggagc
    61621 aaggattcca gccatcgcag gcggcagggg tgatgtttgc cctgcaaatg ggtgcggcaa
    61681 gcgggacgtt aatgttgggc gcattgatgg ataagctgcg tccagtgacc atgtcgctac
    61741 tgatttatag cggcatgtta gcttcgctgc tggcacttgg aacggtgtcg tcatttaacg
    61801 gtatgttgct ggcgggattt gtcgcggggt tgtttgcgac aggtgggcaa agcgttttgt
    61861 atgccctggc accgttgttt tacagttcgc agatccgcgc aacaggtgtg ggaacagccg
    61921 tggcggtagg gcgtctgggg gctatgagcg gtccgttact ggccgggaaa atgctggcat
    61981 taggcactgg cacggtcggc gtaatggccg cttctgcacc gggtattctt gttgctgggt
    62041 tggcggtgtt tattttgatg agtcggagat caagaatgca gccgtgtgcc gatgcctgac
    62101 gcgccttatt aagcaggggc gaaagggagt tgcccccaat acacccacgg cgtttacagg
    62161 tatactcgct aaaaattatt cagcgggttt ggaaacaaag atggcaaaac ttaccttaca
    62221 agagcagttg ctcaaagcag gattagtcac cagcaaaaaa gcggcgaagg tggagagaac
    62281 ggcgaaaaaa tcgcgcgttc aggcgcgtga agctcgggcg gcggtagaag aaaataaaaa
    62341 ggcacagctt gagcgtgata aacagcttag cgagcaacaa aaacaagctg cgttggcgaa
    62401 agaatataaa gctcaggtga agcagctcat tgaaatgaac cgaatcacca ttgccaatgg
    62461 cgatattggt tttaacttca ctgacggcaa tctgattaag aagatttttg tcgataagct
    62521 cacacaggcg cagttgatta atggtcgtct ggcgattgct cgcttgttgg tcgataacaa
    62581 tagcgaaggt gaatacgcca ttattcccgc cagcgttgcc gataaaatag ctcagcgtga
    62641 tgccagcagt atagtgttgc acagcgcgct cagcgccgaa gagcaggatg aagacgaccc
    62701 atatgccgac ttcaaagtgc ctgatgattt gatgtggtaa acatagataa gtcgtgcgca
    62761 gatgcctgat gcgacgctat gcgcgtctta tcatgcctac ggcctgcggg cgaaatgtag
    62821 gcggataagg cgttcacgcc gcatccggca gtcgtgcact attatcaacg catattcagt
    62881 ttattggcgt ggtaggcaat atgctcgcca ataaaactgg agacaaaata atagctgtgg
    62941 tcgtaaccct cgtgataacg gattaacgtc ttgatattca tctcctggca gatcttttca
    63001 agatttggag tccgcagctg ttccgcgtaa aaatcatcac tcaacccctg atcaaccatg
    63061 atttccgcaa cgcgttgacc ttgtgaaata agactcaccg gatcgtaatt caaccaggca
    63121 tctttatttt caccaagata tgcggcaaag gcttgctgtc cccacggtac ttgcgatggg
    63181 gagacaatgg gcgaaaacgc cgagacgctg acatattcat ctgggttacg taacgccagc
    63241 accagcgcgc ccagcccgcc catagaatga ccagagatag actttctggt cgttgccgga
    63301 aaatgttgca tcactaaatc cggcagctcg ttgcggatat agtcatacat tttgtaatgt
    63361 tcattccacg gcgcttgcgt tgcgttcagg taaaacccgg caccttgccc gagatcgtaa
    63421 cggtcggcat ctgcgacatg actgcctcgc ggactggtgt ccggcgcaac aacaataatg
    63481 ttgtgttcag ccgcgtaacg ctgcatcccc gatttagtaa tgaaattctg ctcgttgcag
    63541 gtcaggcccg aaagccagta cagcaccggc aatttttcat tcgcggcttt tggtgggaga
    63601 tagacgccga cattcatttc acatttcagt gattgggaat aatgccgata cacattttgc
    63661 cagccgccaa agctggcatg tttttcaatg agttccatca gtcattatct cacggttaat
    63721 cccccggacg gctgcccagg gagagtatta agaaccccgt ctgaagtgga cggggtatac
    63781 ctgcgggaaa tcagtaacga attacggttc gaatggattt gccttcatgc atcaggtcga
    63841 aggcgtcatt aatttcatca aggctcatgg tatgcgtgac aaacggttcc agatcgatat
    63901 cacctttcat cgcatcttca accatacccg gtaactggga acgacctttc acgccgccaa
    63961 acgcggaacc tttccatacg cgaccagtga ccaactggaa tggacgggtg gagatttcct
    64021 gaccagaacc tgccaccccg atgatcaccg actgacccca gccgcggtgc gcactttcca
    64081 gcgccgcacg catcacgttg acgttaccga tgcattcaaa ggtatggtcg ataccccatt
    64141 tgttgatatc cagcaggaca tctttaatcg gtttgtcgta gtcattcggg ttaatgcagt
    64201 cggtagcacc gaagcgacgc gccagatcga atttcttcgg gttggtatcg atagcgataa
    64261 tccgtcccgc tttcgcctga cgcgcgccct gaaccactgc cagaccaatc gcgccaagac
    64321 caaacacggc aacagaatca cctggctgga ctttagctgt gttgtgcacc gcgccaatac
    64381 cggtggtcac gccacagccc agcagacaga cgtgttcatg gtttgcttct ggattaattt
    64441 tggccagaga cacttccgcg accacggtgt attcactgaa tgtagagcac cccatgtagt
    64501 gataaagcgg ctgcccgttg taagaaaaac gggtggtgcc gtctggcatc aagcctttac
    64561 cctgggtttc gcgaaccgca acacagaggt tagttttgcc agaacgacag aactcacact
    64621 cgccgcactc cgcggtgtaa agcgggatca catggtcgcc aggtttgacg ctggttacgc
    64681 cttcaccgac ttcaaccaca acgccggccc cttcgtgacc gagaaccacc gggaatacac
    64741 cttccgggtc atccccggag agggtaaatg cgtcggtatg gcaaacgccg gtatgggtga
    64801 ctttaatcag cacttcacct tttttcggtg gtgcaacgtc aatttcaacg atttccagcg
    64861 gtttaccggg agcaaatgca acggcagcac gtgatttcat ctctcgctct tcctcaatat
    64921 ggtaatagat tcagctattt aagataggct cgaaccagtt caatagtgtc gtcaacggat
    64981 tggctgactt cgcggctgta gcagtcattt cggtcaaacg tttcccggat atggctttca
    65041 agcacttctg ccatcagccc gttagccgcg ccccgaacgg cagcgatctg ttggagtatg
    65101 gcacggcatt cggcatcacc ctccatcgac cgttccagag catcaatctg ccctcgaata
    65161 cgacgaactc gagtaaggac ctttttcttc tcttccggag tactgggcat ttcgcacctc
    65221 atcatctgca cgcaatatac tatagggggg tatactacat gtcaatgcat acccccctat
    65281 agtatataac actggagaat aaaatttatc cggtgaatgt ggtcggaaaa caaagaggaa
    65341 aggggggggc taatcggcag ggaaggccgc cccggatagc gggcggcaga aggaatcaga
    65401 atttccaggt cagatgggct gcaagttgca gaccgttata atcatcggtt ggggtgtcgt
    65461 accacacttt acctgccgtc agcccgagat taagttgctc agtcagcggt tgaatacgtt
    65521 gcagactgac gctcttcatg ctgccgtagc gtgtttctgg cgtccagtca taggtgtaag
    65581 cgcctgttcc gcggcttagc catagttgcg tataaccagt gccgcgcggg tcattcaggc
    65641 gcacggaaat catattgcta taactaccac ctgcatcgct ggagtcataa tgggtatagc
    65701 ggtagctggt gatcaccggg ccagtataga gtgatacgcc gccttgccag gcatcgactt
    65761 cgacatcatc gtaatattta gtgtagcgat aaccggtcgt gaaaagcgtt tttggcaaca
    65821 gggccaggtt aatatcctga cgaaaattct ggcgggcaaa gaccggcgta ttatccgcaa
    65881 aggcaatacc cgttcgggtt gtcagccagt tattccattt ataccagacc gcaccctgac
    65941 cgcgagtggc attgaaatgt tcagtttcat aatcacgacg cccctgggaa agattaaaaa
    66001 gcagcgttgc gttttcaact ttcgccacca gttcagcata cgctaaatta cggttgccat
    66061 gatcgccaga ataatcggta aaatcgtagc ccgcggtaat ggaagttaac ccggcataag
    66121 cgggcaatgc gctaaaaatg gccgccgcta ataacgtccg ttttatcatt ttaatctcct
    66181 gtacggataa gttcttgtcg gagtgaataa gttccggcag gtaaattacg ttctacaatc
    66241 acgcgcgggc caagttgcgt gttcggggaa ataattcgcc caggcaaaat tataacttgt
    66301 acgccaaggc gtgaacgctg cccgatataa caacctaatt tatcgcatcc ggtagcgata
    66361 attccctctg gagttcgaac agacacaggc tgttcatcca gacgatgatt actggtacgt
    66421 acttgtgcgc ccaaatatgc ctggtttgca actaccgagt cggcaataaa acattgcgga
    66481 ccaatcgttg cttccgcttc aataaccgca tttttaattt cggtggcaaa accaattttt
    66541 acaccattgc tgattattgt gccaggacga ataaacgcat aattacctat caggcagttt
    66601 gcgccaatca ctaccggacc ctgaataacg gcaccatgac aaatacgggt attcgcgccg
    66661 ataacaactt cacctgcagt ttcatcaata attacctggt ctgcaatctg aatatttttc
    66721 tctttaacat taagcttcag atagtttttc agttcctgtg acagaaggtt gggaataagc
    66781 ggttgacgct tccaccagtg cggaatagcg ggtgggtttg tcgcgctgag gttcgcgtcc
    66841 agtaaaaaag gcaataagtc cataaataat ccagatggca taagctaata atacatacac
    66901 aacggaaagc ggcgctaaag gcaccaacaa ccagcaacga tgaaaccagg cgctaaaagc
    66961 accaataaca caaactacgc cgatccagat aagcggaaac attgccaaca ccacaccatg
    67021 cggcccggtg gtgatgaatt cattaaacca ggtagtgaga taaatcccaa cgccataaag
    67081 cacaaccaac agcataggaa atatactgaa gataccgaag cggctaaata aaagtctttt
    67141 atgcaggcgc atacagaccg cgtatcccac aatccaacgc cgccagcgac gccactcctc
    67201 acgcgggctg ttgcattcct gtgggtatac gatgcagcga ttcgcctgcc gaatacggta
    67261 gccgtttgcc actaatgtcc aggtgagatc aaggtcttcg actttagtac gatccgagaa
    67321 accgaactta cgcaatacat cagtacggaa cattccgcag gcaccgctga taataaacgg
    67381 tgcgccgccc aggagctgct gtagcgtgcg cttcataaca atcatcggca actttacggt
    67441 cgcgcggatg tgcggtaaca gccccgcgcc tttcaacgca gtagagggaa tgccgcctac
    67501 ggcatcagca ccgcgctcaa tttctgccag catatagccc attccgtctt gatcgggcgg
    67561 aacataggtg tcggcatcac ttaaaaaaac ctggtcgcag gtggcataat tgaggccatt
    67621 catcagcgca ccacctttac cggtattttt ttgcgtgacg gcaacaaagc ggtcgcccca
    67681 tttgcgtttg acttccgcca tgaccgcttc ggtattgtcc gtggagccgt cgttaacgca
    67741 aattacccgg caaaaataag ggttacgcag tagattatcc agtgactgcg ccagacacgg
    67801 gccttcgtta tacgcaggta taatggcgtc aatacagcct ttcttttgac tgggtttacg
    67861 tcttagcgta cttaaaaacc atagcaacat tgctatagac atacagataa atatccaggt
    67921 tttcatgcaa tttgctcaaa gaatcatttt atgaattaca aagccttcac ccagatcgct
    67981 attgacctgt tgcccgcgaa attgtgcaac tgcacgcagg cgatcatgac gcatataatc
    68041 acgtcgttcc tggcttttat gtttttttaa tgcagcaagt ttgactgtga aatattcttc
    68101 tttaacggat tcaaaaacct gaggcataaa tgaaagccag gtacttgggg tttcgtagcc
    68161 gagaatttgt ggaatagtgc ggcaggcaac cattgaagct tgataaacag cgagatgatc
    68221 ctgatggcgg tcggcatcat gcatggtata tacccgcatg atttcaacat cagaaggaat
    68281 ttgattttta atgatgtctt ccagagcgga aatcatatca ttgagctgta aatgagcgcg
    68341 ggtgtcagca aaattaagat gaatagtttg gtggcaccct aatatcttta gggcgttgcg
    68401 cgattcttca tggcgatcga ttattccatc tgtaccagag ttgccggtag tcatcaccac
    68461 ggctgcgata taaattcctt tttgcgcaag acgagcaagc gatgcgccac agcctaattc
    68521 tatatcgtca ggatgtgcgc caatagcaag gatacccttt cttttatttg ccgaagaagg
    68581 gagggctgaa tctaaaacct tatccactta atgacactcc attttattta ttatactaca
    68641 agcacaacga tgcactcaga gacgtaatct ctggcgcagc cagatgttaa tttataaatg
    68701 ttaaatgttg ctaataactg aaaagcaagg gctttcagac ccggaggttc gactgggtaa
    68761 tgtttttatg caattcatat gttaagtgtt tgtatgtttt gtatgtatag ttattttgtt
    68821 ttatacattg tatttagatg tgatttagtt tgtcaattaa ttgcatttaa aaaatatgtt
    68881 ctgtgaacaa gtattgttta tata
//
LOCUS       DAZKGS010000006        65557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKGS010000006 DAZKGS010000000
VERSION     DAZKGS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..929)
                     /gene="rfbA"
                     /locus_tag="ACHOQY_000407"
     CDS             complement(51..929)
                     /gene="rfbA"
                     /locus_tag="ACHOQY_000407"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9676569.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(987..1886)
                     /gene="rfbD"
                     /locus_tag="ACHOQY_000408"
     CDS             complement(987..1886)
                     /gene="rfbD"
                     /locus_tag="ACHOQY_000408"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFP9676570.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(1886..2971)
                     /gene="rfbB"
                     /locus_tag="ACHOQY_000409"
     CDS             complement(1886..2971)
                     /gene="rfbB"
                     /locus_tag="ACHOQY_000409"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9676571.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            complement(3343..4236)
                     /gene="galF"
                     /locus_tag="ACHOQY_000410"
     CDS             complement(3343..4236)
                     /gene="galF"
                     /locus_tag="ACHOQY_000410"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9676572.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            complement(4411..5805)
                     /gene="wcaM"
                     /locus_tag="ACHOQY_000411"
     CDS             complement(4411..5805)
                     /gene="wcaM"
                     /locus_tag="ACHOQY_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9676573.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(5816..7036)
                     /gene="wcaL"
                     /locus_tag="ACHOQY_000412"
     CDS             complement(5816..7036)
                     /gene="wcaL"
                     /locus_tag="ACHOQY_000412"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9676574.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(7033..8313)
                     /gene="wcaK"
                     /locus_tag="ACHOQY_000413"
     CDS             complement(7033..8313)
                     /gene="wcaK"
                     /locus_tag="ACHOQY_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9676575.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            complement(8459..9937)
                     /gene="wzxC"
                     /locus_tag="ACHOQY_000414"
     CDS             complement(8459..9937)
                     /gene="wzxC"
                     /locus_tag="ACHOQY_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9676576.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(9939..11333)
                     /gene="wcaJ"
                     /locus_tag="ACHOQY_000415"
     CDS             complement(9939..11333)
                     /gene="wcaJ"
                     /locus_tag="ACHOQY_000415"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9676577.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(11469..12839)
                     /gene="cpsG"
                     /locus_tag="ACHOQY_000416"
                     /gene_synonym="manB"
     CDS             complement(11469..12839)
                     /gene="cpsG"
                     /locus_tag="ACHOQY_000416"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9676578.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(13032..14468)
                     /gene="cpsB"
                     /locus_tag="ACHOQY_000417"
     CDS             complement(13032..14468)
                     /gene="cpsB"
                     /locus_tag="ACHOQY_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9676579.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(14471..15694)
                     /gene="wcaI"
                     /locus_tag="ACHOQY_000418"
     CDS             complement(14471..15694)
                     /gene="wcaI"
                     /locus_tag="ACHOQY_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9676580.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(15691..16170)
                     /gene="gmm"
                     /locus_tag="ACHOQY_000419"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(15691..16170)
                     /gene="gmm"
                     /locus_tag="ACHOQY_000419"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9676581.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(16173..17138)
                     /gene="fcl"
                     /locus_tag="ACHOQY_000420"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(16173..17138)
                     /gene="fcl"
                     /locus_tag="ACHOQY_000420"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9676582.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(17141..18262)
                     /gene="gmd"
                     /locus_tag="ACHOQY_000421"
     CDS             complement(17141..18262)
                     /gene="gmd"
                     /locus_tag="ACHOQY_000421"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9676583.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(18288..18836)
                     /gene="wcaF"
                     /locus_tag="ACHOQY_000422"
     CDS             complement(18288..18836)
                     /gene="wcaF"
                     /locus_tag="ACHOQY_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9676584.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(18852..19598)
                     /gene="wcaE"
                     /locus_tag="ACHOQY_000423"
     CDS             complement(18852..19598)
                     /gene="wcaE"
                     /locus_tag="ACHOQY_000423"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9676585.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(19609..20826)
                     /gene="wcaD"
                     /locus_tag="ACHOQY_000424"
     CDS             complement(19609..20826)
                     /gene="wcaD"
                     /locus_tag="ACHOQY_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9676586.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(20801..22018)
                     /gene="wcaC"
                     /locus_tag="ACHOQY_000425"
     CDS             complement(20801..22018)
                     /gene="wcaC"
                     /locus_tag="ACHOQY_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9676587.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(22015..22503)
                     /gene="wcaB"
                     /locus_tag="ACHOQY_000426"
     CDS             complement(22015..22503)
                     /gene="wcaB"
                     /locus_tag="ACHOQY_000426"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9676588.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(22506..23345)
                     /gene="wcaA"
                     /locus_tag="ACHOQY_000427"
     CDS             complement(22506..23345)
                     /gene="wcaA"
                     /locus_tag="ACHOQY_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9676589.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(23438..25600)
                     /gene="wzc"
                     /locus_tag="ACHOQY_000428"
     CDS             complement(23438..25600)
                     /gene="wzc"
                     /locus_tag="ACHOQY_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9676590.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(25603..26046)
                     /gene="wzb"
                     /locus_tag="ACHOQY_000429"
     CDS             complement(25603..26046)
                     /gene="wzb"
                     /locus_tag="ACHOQY_000429"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9676591.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(26052..27191)
                     /gene="wza"
                     /locus_tag="ACHOQY_000430"
     CDS             complement(26052..27191)
                     /gene="wza"
                     /locus_tag="ACHOQY_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9676592.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            27850..29433
                     /gene="yegH"
                     /locus_tag="ACHOQY_000431"
     CDS             27850..29433
                     /gene="yegH"
                     /locus_tag="ACHOQY_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9676593.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(29707..31560)
                     /gene="asmA"
                     /locus_tag="ACHOQY_000432"
     CDS             complement(29707..31560)
                     /gene="asmA"
                     /locus_tag="ACHOQY_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9676594.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(31582..32163)
                     /gene="dcd"
                     /locus_tag="ACHOQY_000433"
     CDS             complement(31582..32163)
                     /gene="dcd"
                     /locus_tag="ACHOQY_000433"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9676595.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(32255..32896)
                     /gene="udk"
                     /locus_tag="ACHOQY_000434"
     CDS             complement(32255..32896)
                     /gene="udk"
                     /locus_tag="ACHOQY_000434"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9676596.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            33214..36524
                     /gene="dgcE"
                     /locus_tag="ACHOQY_000435"
                     /pseudo
     CDS             33214..36524
                     /gene="dgcE"
                     /locus_tag="ACHOQY_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(36563..37420)
                     /gene="alkA"
                     /locus_tag="ACHOQY_000436"
     CDS             complement(36563..37420)
                     /gene="alkA"
                     /locus_tag="ACHOQY_000436"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9676597.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            37554..38906
                     /gene="yegD"
                     /locus_tag="ACHOQY_000437"
     CDS             37554..38906
                     /gene="yegD"
                     /locus_tag="ACHOQY_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9676598.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(38939..40858)
                     /gene="yegI"
                     /locus_tag="ACHOQY_000438"
     CDS             complement(38939..40858)
                     /gene="yegI"
                     /locus_tag="ACHOQY_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP9676599.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(40855..41616)
                     /gene="pphC"
                     /locus_tag="ACHOQY_000439"
     CDS             complement(40855..41616)
                     /gene="pphC"
                     /locus_tag="ACHOQY_000439"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP9676600.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(41613..42272)
                     /gene="yegL"
                     /locus_tag="ACHOQY_000440"
     CDS             complement(41613..42272)
                     /gene="yegL"
                     /locus_tag="ACHOQY_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9676601.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(42493..42552)
                     /gene="ibsA"
                     /locus_tag="ACHOQY_000441"
     CDS             complement(42493..42552)
                     /gene="ibsA"
                     /locus_tag="ACHOQY_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP9676602.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(42825..42881)
                     /locus_tag="ACHOQY_000442"
     CDS             complement(42825..42881)
                     /locus_tag="ACHOQY_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9676603.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(43153..43209)
                     /locus_tag="ACHOQY_000443"
     CDS             complement(43153..43209)
                     /locus_tag="ACHOQY_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9676604.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            43488..44735
                     /gene="mdtA"
                     /locus_tag="ACHOQY_000444"
     CDS             43488..44735
                     /gene="mdtA"
                     /locus_tag="ACHOQY_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9676605.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            44735..47857
                     /gene="mdtB"
                     /locus_tag="ACHOQY_000445"
     CDS             44735..47857
                     /gene="mdtB"
                     /locus_tag="ACHOQY_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9676606.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPLTILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            47858..50935
                     /gene="mdtC"
                     /locus_tag="ACHOQY_000446"
     CDS             47858..50935
                     /gene="mdtC"
                     /locus_tag="ACHOQY_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9676607.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            50936..52351
                     /gene="mdtD"
                     /locus_tag="ACHOQY_000447"
     CDS             50936..52351
                     /gene="mdtD"
                     /locus_tag="ACHOQY_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9676608.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            52348..53751
                     /gene="baeS"
                     /locus_tag="ACHOQY_000448"
     CDS             52348..53751
                     /gene="baeS"
                     /locus_tag="ACHOQY_000448"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9676609.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            53748..54470
                     /gene="baeR"
                     /locus_tag="ACHOQY_000449"
     CDS             53748..54470
                     /gene="baeR"
                     /locus_tag="ACHOQY_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9676610.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            54661..54993
                     /gene="yegP"
                     /locus_tag="ACHOQY_000450"
     CDS             54661..54993
                     /gene="yegP"
                     /locus_tag="ACHOQY_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9676611.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            55202..55498
                     /locus_tag="ACHOQY_000451"
     CDS             55202..55498
                     /locus_tag="ACHOQY_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9676612.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            55500..55796
                     /locus_tag="ACHOQY_000452"
     CDS             55500..55796
                     /locus_tag="ACHOQY_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9676613.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            55899..57260
                     /gene="yegQ"
                     /locus_tag="ACHOQY_000453"
     CDS             55899..57260
                     /gene="yegQ"
                     /locus_tag="ACHOQY_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9676614.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(57590..57907)
                     /gene="yegR"
                     /locus_tag="ACHOQY_000454"
     CDS             complement(57590..57907)
                     /gene="yegR"
                     /locus_tag="ACHOQY_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9676615.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            58322..59221
                     /gene="yegS"
                     /locus_tag="ACHOQY_000455"
     CDS             58322..59221
                     /gene="yegS"
                     /locus_tag="ACHOQY_000455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9676616.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(59303..60076)
                     /locus_tag="ACHOQY_000456"
     CDS             complement(59303..60076)
                     /locus_tag="ACHOQY_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9676617.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(60182..61222)
                     /gene="gatD"
                     /locus_tag="ACHOQY_000457"
                     /pseudo
     CDS             complement(60182..61222)
                     /gene="gatD"
                     /locus_tag="ACHOQY_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(61270..62625)
                     /locus_tag="ACHOQY_000458"
     CDS             complement(61270..62625)
                     /locus_tag="ACHOQY_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9676618.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(62629..62913)
                     /gene="gatB"
                     /locus_tag="ACHOQY_000459"
     CDS             complement(62629..62913)
                     /gene="gatB"
                     /locus_tag="ACHOQY_000459"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9676619.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(62944..63396)
                     /gene="gatA"
                     /locus_tag="ACHOQY_000460"
     CDS             complement(62944..63396)
                     /gene="gatA"
                     /locus_tag="ACHOQY_000460"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9676620.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(63406..64668)
                     /gene="gatZ"
                     /locus_tag="ACHOQY_000461"
     CDS             complement(63406..64668)
                     /gene="gatZ"
                     /locus_tag="ACHOQY_000461"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9676621.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(64697..65551)
                     /gene="gatY"
                     /locus_tag="ACHOQY_000462"
     CDS             complement(64697..65551)
                     /gene="gatY"
                     /locus_tag="ACHOQY_000462"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9676622.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
BASE COUNT        15611 a        17442 c        16432 g        16072 t
ORIGIN      
        1 gttcaaaaat cagtacatct ggaatttctg ttttaattac gttcatttta ttaataacct
       61 ttaatcattt tcagcagata ctgaccataa gcatttttct tcaacggttc agctaatact
      121 ttcacctgct cagcatcgat aaacccttta cggtaagcaa tttcttccgg gcaggaaact
      181 ttcagcccct gacgttcttc tattgttgcg ataaaattgc ttgcctcaat aagactttga
      241 tgtgtccccg tgtccagcca tgcataacca cgtcccatca tggcaacgga taaacgcccc
      301 tgttccatat aaatacggtt aatatcggta atttccagtt caccgcgggc agaaggctta
      361 agatttttcg ccatttcgac aacgtagtta tcataaaaat aaagcccggt taccgcataa
      421 ttactttttg gttgtagcgg tttttcttcc aggctgatcg ccgtaccgtt tttatcaaac
      481 tcaacgacac cgtagcgttc aggatcatta acgtgatagg caaataccgt tgcaccactt
      541 tctttgttaa cagcgacatc cattaacttc ggcagatcgt gaccgtaaaa gatattatca
      601 cctagaacca aagcacaatc atcaccaccg ataaactctt caccgatgat aaatgcctgc
      661 gcaagaccat ctggactcgg ttgcactttg tactgaagat ttagccccca ctggctcccg
      721 tcacccagca gttgttgaaa acgaggagta tcctgtggcg tactaataat cagaatatcg
      781 cgaagacccg ctaacatcag tgtagacaac gggtaataga tcatcggttt atcataaatc
      841 ggtaacaact gtttactgac agccatagtt acaggataaa gacgagtacc cgaaccacca
      901 gctaaaataa tacctttacg cgttttcatt tcatcattcc ttttaattca tcttgctcca
      961 ccatcacgaa caagatgcaa aaactattaa attgctgtag tcgtaaataa ttcgttgagc
     1021 attcgtttca cgccaacctg ccaatcaggc aaaacaagcg caaatttctg ctgaaatttt
     1081 tctgtattaa ggcgagagtt atgcggacga cgagctggtg taggataggc tgttgttggt
     1141 actgcgttga gcttgttgag tgcaagggga atacctgctt tgcgtgcctc ttcaaaaacc
     1201 agcgcagcat aatcgtgcca ggttgtggta ccactggcta ccagatggta caagcctgcg
     1261 acttctggtt tattcagtgc gacacgaatg gcatgtgctg tacaatcagc cagcagttca
     1321 gcacctgttg gcgcaccaaa ctgatcatta ataaccgcta attcttcacg ctcttttgcc
     1381 agacgtaaca tcgttttggc gaagttattt ccttttcctg catagaccca gctggtccgg
     1441 aaaataagat gctttgcgca atattcctgt aacgcttttt ctccggctaa cttggtttca
     1501 ccgtaaacat ttagtggtgc ggttgcatcc gtctcccgcc atggcatatc gccatttcca
     1561 gggaagacgt aatcagtcga gtaatgaata agccaggcgc cgacttcatt ggctgctttc
     1621 gcaatcgctt cgacacttgt tgcgttaagt aattgtgcaa actccggttc tgattctgct
     1681 ttgtctactg cggtgtgagc ggctgcgttg acaataatat caggccgaat gcttcttacg
     1741 gtttcagcta caccttcagg attactaaaa tcaccgcaat aatcagtaga gtgaacatca
     1801 agagcaatca aattacccag aggtgccaga gcacgctgta gttcccaacc tacctgccct
     1861 gttttgccga aaaggaggat attcattact gacggccctc atagttctgt gcaatccatg
     1921 actgataggc accacttttc acattttcga cccattttgt attggacagg taccattcca
     1981 ctgtcttccg aatcccgctc tcaaacgttt cctgcggttt ccagcccaat tcgcggctaa
     2041 tcttctcagc atcaatcgca taacggcgat cgtgtcccgg acgatcggca acataagtaa
     2101 tttgctcacg ataagattgc tctttcggta caatctcatc caacaaatca caaatagtca
     2161 gcacaacatc gatgtttttc ttttcgttgt gtccgccaat gttataagtt tcacccgctt
     2221 taccttcggt gacaacggta tataacgcac gcgcatgatc ttcaacatat agccaatcgc
     2281 gaatttgatc ccctttgcca taaataggta atgccttacc ttccagagca ttaagaatta
     2341 ccagcggaat caatttttcc ggaaagtgat aagggccgta gttattcgaa cagttagtca
     2401 caatggtcgg taaaccatag gtacgtttcc acgcgcggac taaatgatcg ctagatgctt
     2461 tggatgcgga ataaggactg cttggcgcgt aagctgtcgt ctcagtaaat aagggtaatt
     2521 cttctgtatt atttacttcg tcaggatgag gcaaatcacc atagacttcg tcagtagaaa
     2581 tatgatgaaa acggaagcta tttttcttgt cgctatcaag agcagaccag taattgcgag
     2641 cggcttccaa aaggacataa gtaccaacaa tattggtttc aataaatgcc gcagggccgg
     2701 taattgaacg gtcaacatgg ctttcagcag ccaggtgcat cactgcatcc ggctgatgct
     2761 gagcaaaaat ccgcgccatt gcagctgcat cgcaaatatc cgcatgttca aaaacatagc
     2821 gttcagaatc agaaacatca gcaagtgatt ccaggtttcc ggcgtacgtt aatttatcga
     2881 cattaacaac actatcctgc gtattattta taatgtgacg aactacagca gaaccaataa
     2941 atcctgcgcc accagtaaca agtatcttca ctttattatt ccatattact tcagagcatg
     3001 ctgtgaaata agcggctctt aggttgatta ataggggtat taatgcacgc taccgcccct
     3061 ggctttacag ctaccagtgc actgcatgca tgtctacgat gtgacgagcg ttacccactc
     3121 gcgctaaacc cgaaaaattc aaacgctaat tgtcttacca atccgctctg gaaacaagga
     3181 aaatcctgga aaactttgac taaaatccta ttgctaactc gttgttattc tgattgttta
     3241 tataaaacaa cggcaggaat attcgcaaca aattactttc atcacgaatc ttcactgccg
     3301 ttataatttt cttatcaacc gttacatccg gtcagatttt cattatttgc ttaacagctt
     3361 ctcaatgcct ttacggaact tcgccccttc tttcaggttt cgtaatccat acttcacgaa
     3421 cgcctgcata tagcccattt ttttaccgca gtcgtaactg tccccagtca tcagcatggc
     3481 atcaacggac tgttttttcg ccagctcggc aatagcatca gtcagttgaa tacgtcccca
     3541 tgcaccaggc tgagtacgtt ccagttccgg ccaaatatcg gcagaaagta catagcgacc
     3601 aacggccatg atgtctgagt ccagcgtttg tggctgatcc ggtttttcga taaattcaac
     3661 aatgcggctg actttacctt cgcgatccag cggttctttg gtctggatga cagagtattc
     3721 agagagctca cccggcatac gttttgccag cacctggcta cggcccgttt cattgaaacg
     3781 cgcaatcatg gcagcaaggt tgtagcgcag cgggtcggca ctggcgtcgt cgatcacaac
     3841 gtccggcagt accacgacaa atggattgtc accaatggcg ggtcgtgcac acaaaatgga
     3901 gtggcctaaa cctaaaggtt cgccctgacg cacgttcata atggtcacgc ctggcggaca
     3961 gatggactgt acttccgcca gcagttgacg cttcacgcgc agttcaagaa gagattctaa
     4021 ttcataagag gtgtcgaagt ggttttcgac cgcgttcttg gacgcatgag ttaccaggag
     4081 gatttctttg atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc
     4141 gacgatcggt agcatctctt tgggaatcgc cttagtggca ggcaacatat gcatcccaag
     4201 acccgctaca ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt
     4261 tgctgaatta tagcttgttc gatttttttc gccagcatca attaccctga attgattact
     4321 gaattacttg tgatgttacg ccgcttcgtt gtagattgca gtagcattgt tcctaagtat
     4381 ggctccattt ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt
     4441 aatcttttcc acattaacaa tatggtgatt tatcctgtcg atatcgacgg agctttgccc
     4501 tctttcattc accgcatgaa catttgcaag agatagcagt gtttcttttt tcgccataaa
     4561 cacgccgcga acgtctttgc gcatgtcgaa gttcatgctc aatgcgggtc caacagagga
     4621 ttcctgcatc actttgatat tacgcataaa aaggtgttgc ggcttgttgt gtaactccag
     4681 cgacgcacgc ttcatctcaa cgttagtcag cgccacaaag gagacagcat tcccggcgga
     4741 gatttggatg ccgcgcaatt tgtaagcaag gtgggtatta tccagctgaa tattattcac
     4801 tcggaaattc tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat
     4861 cccggcgcta ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc
     4921 gaccgtggcg ttatcaatac ctgctttctt actgaaatcc ggcgtgatat tgcaggcttt
     4981 gatattacga ataacaaaat gtttaccatt ttcaacatgt atcaactgcc gacaatccga
     5041 tcccgtgatg ttcgccacga caaagttttt cactgcctgg tcttccggat agttgttatc
     5101 ataagtgctt cccgcaagac ctatgccgat gccccaattg attttgccgt tagtacagtt
     5161 gatgcgctcg atgacatggt cagatatcaa aatatcacgg tcgttaatcg ccacattcca
     5221 ttcaatggcg tcgccctgta agtcgctgaa cttacaattg gtgatgttgg caccgataat
     5281 ctggttatga aatccctggc gtaagatggc gtaattagcg tggctaacgg tcaggttatc
     5341 gatggtcagg ttacgcatga cccgtttgtt tttgccgccg atataaatct gcgttaccgg
     5401 accaaagccg ctcatcgcca gccctttgat ggtgcagtca gagccgcgca catccagggt
     5461 gatgttatgc atactgccgc cctcctcccc tgtcacctgg ctgccgtcct gtaagacaaa
     5521 tcgccctctg ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc caggagggat
     5581 aaagatgccg gtgttgatat tgaggcaaac caatccggca ggcacgacga ccgtttgccc
     5641 ttcgctgaag gcttgtttaa atgaggcgat ccagtcgcga gggttgtagt cgttaatgtt
     5701 aacgctttgt cgggcgggaa gcgcgcgagc gaaaggggta tggaggaagg caagcaccga
     5761 gcttgccgtc aggaaggtgc gtcgggagag ttttttaaat ggcatgcgtt ctcctctata
     5821 aagcctgcag caagctggcg agttctcgat tgatcacctg ctggttaaaa tcgtgttcga
     5881 ctttttcgcg cgcacgtttg acgaccggag ccagttcgtc ggtgtccagt tggctaaacg
     5941 ccgccagtcg ttgcgccagt gcgcgagcat cgttctcagg caccagccag ccggatttat
     6001 cggcctccac cagttccggt attccgctat gcagagtaga aaccaccgga atgccgaccg
     6061 ccatcgcttc cattagtgcc accggaatac cttccatatc gccatccgca cctgtaaccg
     6121 atggcaacag gaagacatcc gcgtcgtcga gcatcgcttt cacttcgtgg ctcggtttaa
     6181 agcccggcat ctctaccaca tcttccagtt gatattgttc gatgagggtg cgcaggcgtc
     6241 gttcccacgg gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact
     6301 gacggcaggc ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacggaaa
     6361 tgatttccag cggcgttgcg ggcgctttca ccggacgcgg gctaaaacgc gtcatgtcca
     6421 cgcccatgcg cgatacggcg attttttccc tcgggcagcc cattttttgc aaccttccgg
     6481 cccacagatc gcttattggt agcatcagat cgccacggcg gaacagttgc tgatattcgg
     6541 gagtgtaatg gttgagcacc tcccgactgg agatatcaat gccgtggaag atagtggcaa
     6601 ttttgccgcg aatgacaccc agttcgcgga gttttgctgc ggttaccccc gcagggccaa
     6661 aatgagcgat aaagacatcg gcataaaatg gtgttgcgac ctggccgcaa atggcagaca
     6721 aaatcaggtt tcgcgactcg gcaccatagc gtttgaggtt aagtgcctgc caggtatttt
     6781 tacgatgaat gccgcgtaag atctggctgg cacggtggcg cagtttcgcc actttgcccg
     6841 taggttcgtc ctgtaaccaa cgggttctgg cggcaaggtt gtatttcgtc catgccgcgt
     6901 gggtgttctg ggtgtcgcct ttttgcagcg cgacaatctc cacctcaaag cccatatcaa
     6961 taaacgcggt aatttgattg aggacgaagg tttccgacga cagcggaaat ttcagtaaaa
     7021 agaagccgac cttcatttcg cctccctgat gtgttcaagc acagatttca ccatctgcat
     7081 tcccgtctga cgttcgcgac tgacggcttc gttaagtcgc gtgttcagtg ccggaagctg
     7141 gcctaaggta tccgcaacca tcacttgcag gctgccgtct aataaatgac ggatatcaat
     7201 cgccatctcc ggtagtccca gctgctgcat aatcccggcg gatttatgtt cataattgat
     7261 ggcaatcgcc ggggtggcaa agttcattga gataatggcg gagtgcaggc gcgtaccgat
     7321 ggtgagttca caggccccca gaattttgcc catttccaga tcgttgagtt catccatcac
     7381 tacgtggtaa cgggcaggat cgctgatgtg ctggcgcagg ttgagcgcca ccatgcggtc
     7441 gtctttgtta tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc
     7501 gtcgagaatg cgattgacca ccccggcaaa ggctttttca tatgcttgtt gagtggtgcc
     7561 gagacgtttg tcgaacggtg ccagttcgcg cagggtaatt gccaccgttt tctgttgtgc
     7621 ggcaacgtcc agccagtgtt gaacagcata gctggcggtg aagtcttctg tgtggtgatc
     7681 gaccagccac gcggtatcga cgccatgttc aacttttgaa gtggtgatat tgctgcgttt
     7741 catcagttca aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaaaacgta
     7801 gttcgccagt tggttaaatt gctcatcctg gaacgggcca acactgtgac caatcataaa
     7861 cagcgacttt ttcgccataa acgtgcaaag tgcatgttca aactgtggca cgccgtagag
     7921 atcgacaaaa aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac
     7981 gaagtcggtg aatccctggg cgatggcgat attacgcagc ttgccactgt cggtgacgcg
     8041 tgagagcaat acctgatgct ggtagcggcg acggaggact tttttaacgc gcccgacaac
     8101 gcccgccgcg ctgttgtgtt gtttcatttg caggaacagc ggatcgccca ttaccgggcg
     8161 gttgagtagc caggaagaac tgacaggata gcggctcatc acgtccactt cggcatgtgg
     8221 attgaggatg ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc
     8281 gcaagtgtgg ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt
     8341 gaggctgtgt ttgttgtgtt cgttgccgtt ttttgcctga tgcgagcaca gaaccgtagg
     8401 acggataagg cgttttacgc tgcacccggc aaccgttgtc ggaaccgaaa acaacaactc
     8461 accccgcccg taaaagcatt ttcatttttt cgctgcgaca aaactgacgc ttcacttcca
     8521 ccaccagcgg atggcgcgac agcacaatca tcaccacaaa cgccagcaca cccgcggcta
     8581 tttgcaccgc cagcagcatc cccagcgcca gttgcccttt cagcacaatg cccagcgcat
     8641 aactgaccac cagcgttggc agcgagagat aaaacggcag ccacaaactc aggatgtact
     8701 ggcgataact ggaaccaaga accggtttaa tcatcacgaa atagctcaga atagtgttga
     8761 taatctgcac cagcaggaag ccaagcgtga cgccgattgc gcccgccatc tgcccaccaa
     8821 taacaatcgc cggaataaac agaaatgttt taaatacgtt gaatttaaag ctgatatcga
     8881 cccgcgcttt cgccatcagc agcgaaccaa tcgggttacc cacggagcgc agcagaccca
     8941 ccacacacag cagctgtagc accggaataa tgctgctcca cttttcgcca aataccagcg
     9001 gtacaaagtt attcgacacc accatcagcc ccagcagcgc cggaaagttg ataatcccca
     9061 ctaccgacag cagcttgtag aagttgacgc gcagcttttc ggtatcgtcc tgaattttgg
     9121 caaacgccgg aaacaacacg cgggtgatga tcgggttcag tttcatcggc ggcacaacgg
     9181 ccacgttgta cgccaggttg tatcccccag ccacgcccgc gccgagaata cgcgccagca
     9241 cgagtgttga aaggttggtg ttgagatagt tgatgatgct gtccgccgtc agccaggcac
     9301 caaagcgtaa gttcggtgcc accgacgcca gcgagaaatg cagagcgggg cgatagattt
     9361 tgcggccaaa gtagccaaac agcagcgttc tcaccgcact attgaccaga taaccgagga
     9421 tcgcggtcat cgccagcggc cagaaatggg cgctaaccac cgtaaaagta aagcccgcca
     9481 gcaccgcgct ggtttcgatc atgccgattt tgttgaactc cagctctttt tgcatcaacg
     9541 cgcggaactg ttgcccgtgg gggatcacca caaacgctag tgacaatgtt ttaatcaacg
     9601 gtgccaggtc cgggttattc agcatgtcgc caatggcatc actcaacaaa aataccgcca
     9661 cacacgccac gatccccagc ccgacgttca gccagtacaa cgtggtgagt tcaaggtggc
     9721 tgatttcttt tcgctgaata atggagttag cgataccgaa gtcagaaagc gtatctgcca
     9781 gcgcgataat caccagcgac acagtcagca aaccgaactg gtggttgtcg ataatccgcg
     9841 ccagcacagt catttgcacc agcccgaggc cgatgatgat caccgtggca atcgccgacc
     9901 acttcgcgcc gctgatggtt ttttcacgta agctcatatc aatatgccgc tttgttaacg
     9961 aagcctttga ataccgtcag gaaaacgatt ttgatatcga accagacgct ccattcgcgg
    10021 atgtactcaa ggtcgaactc gacgcgtttt tccattttct ccagcgtgtc ggtttcgccg
    10081 cgccagccat taatctgcgc ccagccggta atgcccggtt tcactttgtg gcgcagcatg
    10141 tagccttcaa tgagctggcg atactgttcg ttatgcgcta ctgcgtgcgg acgtggaccg
    10201 acaatcgaca tccccccggt cagcacattg ataaactgcg gcaattcgtc cagcgaggtg
    10261 cggcgcagaa agttccccac tttggtgacg cgcggatcgt tctgcgtcgc ctgagtcacc
    10321 actttgtcgt tctccatcac tttcatcgaa cggaacttcc acactttgat cggcttgccg
    10381 tccatgccgt agcgagtctg gcggaaaata accggccccg gtgagctcag tttcaccgcc
    10441 aacgcaatgc agcacagcac cggggagatc agcagcagaa tcagcgttgc cagcacaatg
    10501 tcttctgcac gtttgagcag gcggttaacc ccggaaagcg gcgtgtcata cagcggcacc
    10561 actggtacgc cgttcatctc ttcaatacgg gaatggagaa tgttgaaggt aaagacatcg
    10621 gggatcagca gcaccgaaca ggtagtgtcc gccagttgat ggaccagttt tttcactcgc
    10681 gcgccgtcgc acatttgcat cgcgatatag acgttatgaa tcttgcccgc tttcgcatcc
    10741 tcgaccagct gttgcagatt gcccgcccag tcgttagaaa cgccacccgg tttcgggtcg
    10801 tggtaaacgc ccaccacttc aaaccctaac cacggctgat tacggaagct ctccatcagc
    10861 atttgtccgg cggctaaatc ccccgctacg gcgaccatac gcttgttata gccatgatta
    10921 cgcagccagc ccgcgccaat gcgaatacat gaacggcaaa ccaccagtcc gatgctggtc
    10981 agcccatacc acgccagcca gattttcagt tgcgtgtcga aatcattatt gaacgccacc
    11041 agtccggcgc tgaaaatcac gcttaaggtc cagttttgta gcaacagggc aaattctgtc
    11101 gctgcccgaa caccgcgcca tgagcgataa aaatcggtga tgccgcccag catctggaac
    11161 accaccagcg taatcaacgc caccaacagg tgcatgtaga ggaatgacag tccgctcact
    11221 tcgcaaacca gccatagtcc ggcaaacatg atggtgatat ctgaaaagcg ttgcaccata
    11281 gagattaacg atgcattggt tttcgctcgc tcgcgctttt ttagatttgt catcgttgtt
    11341 cctgttctat tgtcgcctcg cccctcaccc cggccctctc cccaaagggg cgagggggag
    11401 aagaaagttc cgtgctcact tctctccgca ggcagtagag cgtcagggtg agggggaaag
    11461 taatgacatt actcgttcag caacgtcagc agagttcgcg ttcgcgcttc catcagcggt
    11521 acatcaccgc gcgattccac attcaaccgc accaccggtt cggtgttgga ggagcgcaga
    11581 ttaaagcgcc agtcggcaaa ggtcatgctg atgccatcgg tgcgatccac cgccagcgcc
    11641 tcacggctaa agtgctgttc cacgcggtta atcgcctcaa cgggttgcgc cagtttgctg
    11701 ttgatctcac cgcttgccgg aaacgccgcc atccggtcgc gcaccagttc gcccagcgtt
    11761 tttcctttca ggcacaccag ttcggcgacc agcagccatg ggatcatgcc gctgtcgcag
    11821 taagcgaaat cacggaagta atggtgggcg ctcatttcgc caccgtagat ggcgtcttcc
    11881 ttgcgcatac gttctttaat aaaggcgtgt ccggttttcg acattaccgg ggtgccgcct
    11941 gcggcagtca ccacatcaac ggtattccag gagagacgag gatcgtggat gatcttcgcg
    12001 ccgggatttt tttcgaggaa cgcttctgcc agcaggccga caatgtagta accctcaata
    12061 aactgccctt tttcgtcaaa caggaagcag cggtcaaaat cgccatcaaa ggcaatgccc
    12121 atatccgcgc cgtgtttgat gaccgcattg cgggtgtcgt cgcggcattc cggcagtagt
    12181 gggttaggaa taccgttggg gaaattgccg tccggcgtgt tgtgcacttt gattaattcc
    12241 acgggtgcgc cgagggcttt aaagcgggct tcaatggcgt ccaccaccgg acccgctgcg
    12301 ccgttcccgg agttgatcac cagcttgagc ggcgtgaggt ttttgacgtt gatataaccg
    12361 aacaggtgat caacgtaagc gtcacgcagg ttgatttgct gatagcgacc gcgtttggtt
    12421 tcatcgacgg gagggaagtc gttggcttca gccagacgct ggacgtcgcg cagtccggta
    12481 tctccgctga tcgggcgagc cccctcgcga accagcttca tgccgttata atccatcgga
    12541 ttatggctgg cggtaacttc aatgccgcca tccacgccga gatggaacgt ggcgaaatag
    12601 atctcttcgg tgccggacat gccaatatcc agcacatcga cgcccgcatc ctgtaaacct
    12661 ttcgccagcg ccagttttaa ggtttcgctg gtgaggcgga catcaccgcc taacacaatg
    12721 gttttcggtt tgagaaattc gccataggcg cgaccaatgc gccaggcaat atcttcattc
    12781 agttcttcgc ctaatttccc gcgaatatca taggctttaa agcaggttaa ttttttcata
    12841 tcgttaccct ttttcaggca atagttggcc ctgaccgaaa cggccaattt tgtttttgtc
    12901 gttatttaca tcgcggtttc gcattcattg cctgatgcga cgctgccgcg tcttatcagg
    12961 cctacaaatc cgaaccgtag gtcggataag gcgttcacgc cgcatccgac aaacagcgcc
    13021 tgatgcgacg tttacacccg tccgtagcga tccgcgaagc gcaccacatc atcctcttcg
    13081 agataagagc cggagcgcac ttcaattaaa tcgagcggaa ttttccccgg gttttccagg
    13141 cagtgcgtcg cccccagcgg aatataaatg gactcgtttt caccaagcag tttgatatca
    13201 ccgtcaatag tgacttttgc cgttcccgcg accaccaccc agtgttccgc gcggtgatgg
    13261 tgcatctgta ccgacaagcc ttcgcccggt ttcacggtga tgcgtttcac ctggtagcgg
    13321 tcgcccgcgt cgatagagtc atatttgccc cacggacgat acacttcgcg atgtacccga
    13381 tgctcatggc gaccatcggc tttgatctgc tcgaccactt ttttcacatc ctgcaccgcg
    13441 ttacggtcgg caatcagcac cgcatctttg gtctgcacta ccaccagatc tttcaccccg
    13501 acggtggtta cgaggccaga ttcggcatac acatagctgt tttctgtttt gtgattaatc
    13561 acatcgccgt ggcaaacgtt gccctcggcg gtgtgggcgc tgatctccca taatgaagac
    13621 caggaaccga catcgctcca gcccgcatcc atcggcacca caacggcatc tgccgtacgt
    13681 tccatgaccg cgtaatccac cgactcttcc gggcaggcga gaaacgcttc ttcatccacg
    13741 cgaataaaat cgagatccgg atcgacggcg ctcatcgctt tttcgcaggc atcgaggata
    13801 tccgggcgat attttttcag ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg
    13861 ctgttccagt aatattcgcc gcttgccaca taggcctggg cggtttccag attcggtttt
    13921 tcgacaaact gcgccacttc aaaggccacc gtatcctgct cacccgcaga cacttcaccg
    13981 cgacgaatat agccataacc ggtttcaggt agatccggca caatgccgaa ggtcaccagc
    14041 ttgcccgctt cggcatatgg catggcatta cgcacggcgg cacggaacgc gtcttcatcg
    14101 gcaatcacat gatccgccgc caataccagc attaacgggt cgctctccgg gctatgacgt
    14161 tttgccgcca gcgccgccag tgcaatggca ggtgcagtgt tacgccctgc cggttcgaga
    14221 ataatgttct cggtgagttt gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc
    14281 tgctcattgc aaatcaccac cgggctttcg cactccacgc cgttcaggcg gcagatggtg
    14341 gtttgcagca tggtgagatc gcctttcagg cataaaaact gcttggggta aagtacgcgg
    14401 gaaagcggcc ataagcggct accggagcca cctgccatca caactggata gagtttcgac
    14461 tgcgccataa ttatccccga atatcattta taaattgacg taacacgttc tctttatcga
    14521 gcgtgcgttc ggcatattca cgtgccaccg tgttgtgttt gggcagcagg agcgcctgac
    14581 ggatccccgc caccagcgcc tcgaccgatt ccggttctac gcaaaccgca atgcccgaaa
    14641 aggtttcgca aagctccccc agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc
    14701 ctaccgccag aatattggtc agtttcgacg gcaatacggc atctgccgcg ccgcgttttt
    14761 gcaccaccag atggcaatcg cccatcttca gcagtgcggg taaagcgtca tacgattgca
    14821 gcgggaaaaa ttgcatgttg tgcagtccac gctgctgcgc cattttttcc agtcgcgctt
    14881 tgccgccacc ctgcccgaca atggcaaaaa tcagtggttc atcgcgcagg cgatcggcag
    14941 cttcaataac gttttccagc ccctgctttt caccaatatt gccggagtaa agaatgattt
    15001 ttttgttatc cggcaggcca agctggttac gaagggcatc aacatcggca tctgcaacat
    15061 gctgaaaacg ggcgatttcc gaccagttgg ggaagaagat gacgttttcc gccgccacgc
    15121 ctttttcgat ggctttattc atcatcgaac gcgaaatcgt ggagacgtta tcgacgttat
    15181 gtagtccgct acgttcgaac gccgttgcca gctgtgccac tttgccgcct ttgccttttc
    15241 cggcaaggcc cagccccagc atggcatcta cttcgtaatc ctgaatatgc agcacggtac
    15301 gcgcgccaga gagtttcgcc agcagacgca ttcccggcgt gcaaaacagc gttggcacta
    15361 cgccgataat gcgctccggc ttccagcgac gttgcgccat cagcgggaaa aaactgctga
    15421 cggcaaaact gccgagatgc aacaggcgtt tcagggtgct cggctgtttt ggcacataca
    15481 gcgggcagcg ccacaccgtg gcggccccct cttctcgttt gtagcgccag gcggaatagt
    15541 tctcgcccac ctgccactgc gggtagtaag gcggtgcggt aatgacccgc acctcatgac
    15601 cttgtgccgc cagccattcc accatctcgc cggtgtattt gccgatgccg gttaactccg
    15661 gcgagtagtt aatgccgtag accagtattt tcataatccg ggtactccgg cacgcttctc
    15721 agcgagaaaa taggcgcggc tgttagcgtg aacattgtca ctggcgagca gcgcgtccgg
    15781 cgtcagccag cggtaatcgt catgctgctc atccggcagt aacagatctt cttcagcgac
    15841 tctgaagcga aaaccgagca ccacatagtg agtggtgaaa tccgtgccgg agaagttatc
    15901 gtcataaaag tgctgccaga caccgtaaaa ctggcctgct gttatcggca gacgcagccc
    15961 cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc gtttcgtctt tctgcacgcg
    16021 ccctcccggc acaaaccagt aaccctgcgc cgggcggttg gttcttttgc caagcagaaa
    16081 ctcgccgcga ctgttctcga caataaagtc gagagagaca agcggtgtgg agcgcactac
    16141 cgtggcaaag tcttcctgac gtaaaaacat cattaccccc gaaagcggtc ttgattctca
    16201 aggaaccact ggtaagtgct ggcaagcccc gcttccagtg atatttcgtg ataccagcca
    16261 agctgatgca ggcgcatcac atccagcagt ttgcgcggtg tgccatccgg tttgctggca
    16321 tcaaaaacca cccgaccttt gtaacccacc actttggcga tggtttgcgc cagctcgcgg
    16381 atagtgcagt caacgcccgt gccgacgtta atgtgcgaca gcatcggctg ggtgttctcc
    16441 agccagactt cgtgcgccag ctccatcaca tgaatgcttg ccgccgccat atcatcgacg
    16501 tgcaggaatt cacgcatcgg tgtaccgctg ccccacacca ccacgtccgg cgcattctgt
    16561 gccgtcgcct cgtggaagcg acgcagcaat gcagggatca catgcgaatt actcgggtgg
    16621 aagttgtcgt gtggcccgta caggttggtc ggcatgactg aacggtaatc tcgtccgtac
    16681 tggcgattgt aagattcgca caatttgatc ccggcgattt tggcaatagc ataaggctcg
    16741 ttagtcggct ccagcgtgcc ctgcaacaac tcgctttctg ccatcggctg ttttgccagt
    16801 ttcgggtaga tgcaggacga tccgagaaac agcagtttgt tcacgtcgtt ctgatgcgcg
    16861 gcgtgaatga tgttgctctc aatcatcatg ttctggtaga tgaaatccgc cggataggtg
    16921 ttgttggcaa caatgccgcc cactttcgcc gccgccagat agacctggtc aatacgttcg
    16981 ctggcaaaga aatcatgcac ggcgcggctg tccagcaggt tcagctcgtc gcgggtgcgt
    17041 aataccagtt ccacatcacc gcgctgttcg agctgtcgct tgatggcgga accgaccatc
    17101 ccgcgatggc cagcgataaa aattcgttgt ttactcatgc ttatgactcc agcgcgatcg
    17161 ccacgtcgta gccgtgagat ttcagcagag agtgtttttt cgccgcttcg aggtcattag
    17221 ccaccatttc agacaccatc tctctgaggg tgatttccgg tttccagccc agtttttcgt
    17281 gcgctttggt cgggtcgccg agcagcgttt caacttccgc cggacggaag taacgcgggt
    17341 caacggcgat aatcacatcg cccggtttaa cgcccggcgc gtcatgcccg gtgacggaaa
    17401 ccacaatgcc cttctcttca acgcccgtgc cttcaaagcg cagtttgatg cccagctgtg
    17461 ctgccgccat ttccacgaac tgacgtacgg agtactgaac gccggtcgcg ataacgaaat
    17521 cttctggctg ttcctgttgc agcatcatcc actgcatttt tacgtagtct ttggcgtggc
    17581 cccagtcacg cagggaatcc atattgccga ggtacaggca cgactccagc ccctgagcga
    17641 tgttggcgat tgcgcgggtg attttgcggg taacgaaggt ttcgccgcgg cgcggggatt
    17701 catggttgaa gagaataccg ttacaggcgt acatgccgta ggattcgcgg tagttaacgg
    17761 tgatccagta ggcgtacagt ttggcgaccg catacggaga tcgcgggtag aacggcgtgg
    17821 tttctttctg cggaatttcc tgcaccagac cgtacagttc agaggtggaa gcctgataga
    17881 aacgagtttt cttttccaga ccgaggaagc ggatcgcctc cagcaggcgc agcgtaccca
    17941 tcgcgtcgac gtcagcggta tattctggtg actcaaaaga gaccgcaacg tggctcattg
    18001 cgcccaggtt gtacacttca tccggctgta cttcacgcaa aatgcgcgtc aggttagagg
    18061 tatcactcag gtcgccataa tgcagatgga atttcgggtt gcaggtgtgc ggatcctgat
    18121 aaatgtgatc cacgcgctcg gtgttgaatg acgatgcgcg acgcttaata ccatgcacct
    18181 cgtaaccttt ttccagcaga aactctgcca ggtaagaacc gtcttgtccg gttacaccgg
    18241 tgatgagagc gacttttgac atgtcttatt cctctgtatt tttgaattta ttcagtttca
    18301 acgcgttcgc gtatcaccac tgcgggattc ccccggcaaa ccacatttgc cggaagcgat
    18361 ttaaaaacac tgcttcgtgc acccacgacg gtgccgttgc cgattgtgac gcctggggca
    18421 acaaagacat cggttgccag ccagcatttc tcgccaatca caataggcgt ggcgttaatg
    18481 gtgaaatgtt gacttgcatg gtcgtggcta ccggtgcata aataactttt ttgcgatatc
    18541 accgaatgtg cgccaatggt tatttcaccg agggtatata aattgacgtc atcgccgacc
    18601 cacgcgtaat cacctaaggt taatttccac ggataggtaa tttttactga cggacgaata
    18661 actacgtttt ttcctatttt tgctccgaat aaacgtaata aaaaagcccg ccagcgatac
    18721 aatacttgtg gcgaccaggc aaatattgtt gcctgtactg cccaccataa ttgcacttta
    18781 atagcgttgc cgccccggaa ccctttcggc accgagaaac cgcttaaatc ttgcattggt
    18841 tctccttata ttcagacttt gttatataag gctttcgtct ttgaggtagt acgttggcgt
    18901 aaatgccagg ataattcagc ccaaaagcca ggtacatgta atatttgtcg ttggactttt
    18961 ttcgcgtcag cacacaattc catattattg gtggtagata ccccacccat ggaaaattca
    19021 gacaccaggc cattgagttt tttaaatgca taaccggctt tatacatttt ggctgccagc
    19081 gcgtagtcgg aagaaacttt atattccagg tcataacgcc attttttcaa gccggataca
    19141 gggaaaaata tcgcctgatg actggcgggc aggctgtgat aaatatacca gcccggtttg
    19201 gcgctacgtt taattttatg cccgtcgcca aaatccagca gcgcatcgcc ggtgatcatc
    19261 atgttatctt tttgcatttt taacttacgg acaaaatttg cggcatcctg atgaaaaata
    19321 tcgcccgaat tgagaaacaa cgcgaacttg ccttgtgcca tcgtaatgcc tttgttcatg
    19381 gcgtcgtaga taccgttatc tggctcgctg acaaagcgta ggttatagat accattgaga
    19441 ttttccagat actcacgggt gccgtcattg gaaccgccat cgacaacaat ccattcgaag
    19501 ctgatatctt ccgcctgcgc cagatgcgcc agcgaggcat gtgttttgac tatcccttcg
    19561 aggttacgaa acgcgacagt gattatgcta agcaacatgt tcttattctt acctcgtaat
    19621 atttaacgct tttcgcaaaa taaacggaca gacgattaaa aatgcatatt ccgggctaaa
    19681 tatcgaaccg gtaaaaaaca gcgacaccgg agtaaaaaga tataattgca cgcgaaaatt
    19741 gcggttatca ccaaaagcgt tgatcatcat ttttatcact ttccccatgt accacagcga
    19801 taaaaacact gcgaaccagg aaaaataaat aatcagcaga tacaaaccat tgtctatggt
    19861 ttttccgaca tccgcaccgt taaatattcc gaatgatgcg acatattcat aaagtgagcc
    19921 aaatctgact acaccgtcaa tatgggtcaa agaataaccg accatcacca gcggaccgac
    19981 aatacgataa tacgatgacg atccttccgt acctaaatcg cccagacggg tggaaatata
    20041 aggaaacgcg attaccacac caaccaggaa tacagccaga gaaatcaatg ctaacggtaa
    20101 ctttttctta atcgcctctt tattcagata ttgaaatgcc cactccagca aataaaacag
    20161 gataaaggtc ataacccctg aaaacgatcc ggataatatt atccctgcga gaatcatagc
    20221 atcggtttta ggcgttttga taccaaactg tttgatgctg agccaaattg agattaatgc
    20281 cagagcgaaa aatgccggtt cgaaataaag tgctgtcgtg cgcttgccgc cgaatttaat
    20341 gaaattcagt acatagctgt tgctgtaaat cagatatttc gaaatcgact ccataatact
    20401 gctgccgccg gtgaggataa tttgcgccat ctccaccgcc gccagcgcca ccaccaaccc
    20461 gaccaccaga taaaagaaac gtaatatctt gcgatggttg tgtggcgaaa ttgttttaaa
    20521 acgaatactc cacaccatgc caataatgat cacaatatag acaaacagca tagttgaagt
    20581 gacgtattta ctggcatcca gcgactgacc aaacagatag ttaaacgccg tgagtcccgc
    20641 accaatccct aaggcaatca tcaatttttt aacgctgatg cgctctaaaa acagcagtaa
    20701 caagacaggt aaaaaagtga cgatggtgat ggggaaactt tcgccaagct gggcgatttt
    20761 gacgttaacc agcagataaa tcagcggcaa caacaggtag ctacagattc tgatagaagt
    20821 tgacatactc ctccagcatc tgttgtccac tgtaggccgc gcggctgcgt tggctgaact
    20881 cagccagcgt ggtaccaaat atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt
    20941 gcagcacgtc ttcttcgctg acggttttac cgccggattt ttgcagcact tcccgcgccg
    21001 cgtcgctatg ggtggcaatc accggcacgc caatcgatag cgcctcacac aaaatcagcg
    21061 ggtagttatc gacgcgagaa ctgaacacca gcgcatccat ctgattgagc gcgctcatca
    21121 acttgcgctt gtcagtttca aagccgtgat taaccacgtt gccagcggtg aacggcgaga
    21181 acttaccaaa ggtatgcagt tcgattttgt cgcccagcgc catcatctca cgcaccagtt
    21241 gctggttagt tttgccgtcg taacgcaggt catgcgccac caccgcgatt ttcggcttac
    21301 cctgggtttc gcgcaccgga ggcagatccg ccagaatcgc ttcggttgcc atatcaatac
    21361 cgttattgat aatccggcaa cgccctggac cgtacaggct attgaaagcg tcagccacat
    21421 gctggctggg ggaaataaac tgacagccca gcgccagcat ctcacggaat aactggcgtt
    21481 tgcccgccac cagctggtgc gcccgatcaa tcttcaccgg cggataatta tttaaggtcg
    21541 ggcatttctg gcagcccgtt ttccagcctt cacaaccgtc agtaaaggcg cagcgcccgg
    21601 taacgctcca gtggtcgtgc agcgtccaga ccagagtgac atccggtttg tggtttttca
    21661 ccttttcgca aaagcgcacc acgctcttaa gatttagcca gtagctgtgc agcacatgaa
    21721 aatgcaggac caccggaccc ggtgtgcaag taatggtgcg atataactca ttgaaattgc
    21781 caaacagatc gcgattaaac agacgaaaca acgcaatgtt cgccatcgcg gtcatccgcg
    21841 gcgtatgttt gacgacctgc ggatagtttt gatggctgac gctctctttg ccgcctttgc
    21901 cgtagccgta gacaaaatgt gacgccagcc cctgttgcag cgcacgctga tggagatcta
    21961 acgccacacc tgccgccccg ccttccgcca gtcgcacatt aaattgcaga atattcattt
    22021 aattaccttc actcgcgctt tttctcccac caccagcgcg ttgtccggga cagagtcgag
    22081 caccacgctg cccgcgccca cggtgacgtt gttaccaagc gtgatatcac caagaataat
    22141 gacgttggca ccgagttcga cgccgttgcc aatgtgtgga catgccatgt tatcggcacc
    22201 acgattgccg atagtgacgc cgtggcgaat ggtgaaatca tcccccgcta ccacgttttt
    22261 attgatcact acggcgtaac cgtgatggat agtaaagcgg cggccaatgg tcgcggcggc
    22321 ctggatttca taaccgaaaa agcattcggt gataatgcga tacagcacca gcagcggggc
    22381 cgcccacaga ttgttgagga cgttcttttt gcgccacacc gaacaaaaat gagcaacacg
    22441 ataggcaaga accatgcagc acgggcgtaa actccagctg ttggcacgca gatcttccag
    22501 catctttagc gcccccgaat accatcagcc agacgtttgc cgttacgcac cgacagcagc
    22561 gtcagcaaag tgcgccaggt catacgttta ttgcggatct ggtagagggt aaacagctga
    22621 tattttttgc ttgcacggtc aaatttatct ttgtgcttgc ggtaaaagtg gaagtacccg
    22681 gagaattttt tcggtgacga ggtgatctgc atttcaccgt gattgatatg caggatctgc
    22741 gtcgcctctt ccactttcca cggttcgccg tactccacca ccatccgcag gaagatgtcg
    22801 taatcctgtg ctgctttcag ttcggtatcg aacaaacact ctttgaaacg ccatgcccag
    22861 gtaaagacct gattgccaat gatattgcgt ttatagaaca ggcggcgtga gtacggcgat
    22921 ttgggataca gtggtaagct tgccggttgg gaataaactt ctccctggca tacgtagtcg
    22981 ttagcgtaca aaaaggcgtg tgtgaccagt tgctgtttat gggcgaggaa gacgctcaga
    23041 cggttgggtg tccattcgtc atcgtcatca atcccggtga tatattcccc ttgcgccagc
    23101 ataatcgcct ggttacgtac cgcgcacgcc ccgctgttaa tatcgttgtg aatgtaagtg
    23161 atacgcggat cgttgagggc ggtgacgtac tgttgcagtt gttcccagga ggtggagcaa
    23221 tcatccacga tgatcatctc ccagttgctg tagtcctggc gcaaaaccga ttttattgcc
    23281 cgaatcgcca gttgttggcg gttccaggtt ggcatataga ttgagatcag cggattgtct
    23341 ttcataggtt gttctcccgg cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc
    23401 ttatccggcc tacggggcgg tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt
    23461 cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg gagttcagaa
    23521 tcaccccttt caccggaata ccattttgct caaagcggct cagactggtt tccacttctt
    23581 tcaatgtgtt gaccgcataa cgcgccacca ttaacgtggt tccaacatga cgaccaacaa
    23641 ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg tcatagtttt
    23701 tactcgccca gttcaccagt tcggcaaagc gttcgctcat caacagttca gaaggatttg
    23761 gcggcacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt ttagcagcgg
    23821 tagtaatatc gccctgacca atcagaattt ccgacaggcc attaacgtta ttagtgccca
    23881 acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacg cgtttattgg
    23941 tctggctgat caccgccgcc aggttggcgc agacaaaggt tttaccgatt gacgggctaa
    24001 ccccggtcat catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc aaactggtac
    24061 gaagactgcg gatggcttca atcgccagat cggttggatt ccccaccgcc agtagctggc
    24121 tctgtttata gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct ttctgccatt
    24181 ccgacaacgg gatgctggca tagacgctga taccgtgttc ttccagcacc tgcgggcttt
    24241 cgatgccgcg attaaacaac gagcgcagca gcacacccac gatagagagc atcaggccaa
    24301 ggataatcgc gccgaggata atcagccctt tcttcggttt tagcacacca ggctgagtga
    24361 ttgccgggtc aacaatgcgc acatcgccga cggtgctggc ttcggtgatt ttcagctcct
    24421 gctctttatt cagcagttgc atatagacct gctgaccaga ttcgacatcg cgggtcagac
    24481 ggacaatttc ctgctgggtt ttcggcatcg ccgttacgcg accattaagt ttggcttttt
    24541 cgtcttccag cgcctgacgt ttctccagca gcgtgcggta cgcggggtga actttggtgt
    24601 acagcttgga gatttccgcc tctttaaagg tcagttcgtt caactgcgcg tcgatgttca
    24661 ccatcgaatc gagcaccgct ttcgcttcca gcggcagatc aacagaatct ttatcctgac
    24721 ggaaggcatt cagtttgttt tcggcaacat cgaggcggct acgtacttcc ggtaactgtt
    24781 gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt gcgctcaata ttttgttcct
    24841 gatagttacg ggcgatgctg ttaagaatgt cgcggatctg ttcacgatct tcaccggtat
    24901 aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccgtcagg ctgttttgca
    24961 gttgattgat catccccagc gtggagtatt tggtgacggt aaactcactg cccgggcggg
    25021 cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg cccgcttgcc
    25081 cacgggcgct aaagccgcca tcactgctca gggtgtagtt tttattgtcc agcacattaa
    25141 gcgtaaacac ctggtccgcc atctctttcg ggcggttaaa ggtggtcact ttcaccgtct
    25201 cgttctgacg tcccatcaaa cgatcccagc ccgcaccgaa aatcgggaac gtgtttttgc
    25261 tcaccgcaat atcgaggtct agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa
    25321 tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc taatgccgag ccaatgtcct
    25381 gcactaacga attgccgctg ttttgttcga tttgtaccag tgcgtcggca ctataaatcg
    25441 gcgtggcgaa gaaagtgtaa accacagcac agagggcaaa tacagcggtg atgccaatca
    25501 cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt tcatcactgc
    25561 ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct gctctgcgtt
    25621 caatgcctgc gcccactggc gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt
    25681 ttcccggctt ttgcgatacg gatcggggat ttcacattcg ttatcccagt gaccaaacag
    25741 catcactttg ccgcgcatct caggtgccat ctcgcataag cgttcgatat ggcgcttttc
    25801 catggtcaaa atcaggtcat agttgcgaca caggcggcgg ctgatttgcc gtgcacagtg
    25861 accttccaga gacagttgat gttctgcggc gacgctgata gcggtaggat cagcgccctt
    25921 accgaccagc gcgccgagtc cagcggactc cactttcagc tccgggtgat aacgttgcag
    25981 taagcgttcc gccgtcgggg aacggcaaat attgccgaca cagacaacta agatgttgtt
    26041 aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta acggacaccg
    26101 ctaatagttg gcagcaactg attgatcaga cggttccagc gggaaaccgg ggcggtggtg
    26161 acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag cgacgtcgca
    26221 tcggacatat ccagctggta gatattggca atcttgccgt tacgcccgcc ctcgcctttc
    26281 aacggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat gccttcggca
    26341 ttgcccagag cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc
    26401 ttcacttcac ccatcacaaa tactttcaga tcatcattac gcggcacgta gagaatatcg
    26461 ccgggataaa gcagtcggtt ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa
    26521 atgcgttctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc
    26581 aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt
    26641 tgaccggatt tattcacctg acctgagata taggcctttt gcgagcggaa ggcggcgata
    26701 ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat atcactgcgg
    26761 atttcagcga gcgttttccc gactacgtga accttgccga tatacgggta aaacatagtg
    26821 ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg
    26881 gtgagttccg ggtgatccca gacggtgaca ttaagaacgt cccccggccc gacgcgatac
    26941 tgataattcg cgatctcgct ttccagcgtc atattggggc gcgctacatt cgggcgtggg
    27001 cgtaattggt caatcaggcg cggggtcagc ggataaacat tcaccatttt gtcgagatcg
    27061 aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg tcgacatatt gctgcccgga
    27121 agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa tttcattttg
    27181 gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca ctattaatag
    27241 caatgtcgcc atgcacattt accttgcagt taattgaata aaaatttaac tggcatcagt
    27301 cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact ggtctttcag
    27361 gcatccagac acgctaccgc ccctggcttt ttagctacca atacactgat ttagcttaat
    27421 ttttcaaacc ctctctgcat acaatcgttg atgagaaagg gttattgcgg aaattaactt
    27481 ccgaatataa ggtgacatta tggtaattga atattggctc tccaataatg caggaggaag
    27541 tgttacagct aacggaatag caggcaaggt aacaattcgg taattggcta tttttaagaa
    27601 ttatattaag tgtcattcaa tatggttttt aggagtttct ttaggttgac aatatttaat
    27661 attgcgcctc cacatgcgat atttcttaaa taatgtttta ttattaccac ttttaattca
    27721 gggataatgt gaggttatta ccctcaaata atatgagata aatagtgctg caacattgca
    27781 tttttgcccc gatttatcca tgatcgaatt atgacatttg tcatacaacg aataggtttt
    27841 gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat cacgctgatt
    27901 gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct cgccgaaaaa
    27961 ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg
    28021 cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc tgactcaacc gctgttcagc
    28081 ttctgctcgt ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg
    28141 ttgttcaaag ccacgatgga gctgaacgaa cggctggaag ggaaagacag caataatccc
    28201 acacaacgca aaggtgcgaa gttctggggc gtggtgacgc aaattgtggt actggacgcc
    28261 atcttctcac tcgactcagt gattaccgcc gtcgggatgg tcgaccattt actggtcatg
    28321 atggccgccg tggttatcgc tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa
    28381 tttgttaaca gtcacccgac catcgttatt ctctgcttaa gcttcctgtt gatgattggc
    28441 tttagcctgg tggcagaagg tttcggcttc gtcattccga aaggctacct gtacgctgcc
    28501 attggtttct cggtgatgat cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt
    28561 tttctttccg ctaaccagac gctgcgccag cggactaccg aagcggtaat gcgcctgctt
    28621 agcgggcaaa aagaagatgc ggaactggac gccgaaaccg cgtccatgct ggtggatcat
    28681 ggtaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt acttaatctt
    28741 aaccagcgta ctgtcagcag cattatgacg tcgcgccacg atattgagca tatcgatctc
    28801 aacgcgccgg aagaagaaat ccgccagttg ctggagcgaa atcagcatac gcggctggtg
    28861 gttaccgacg gtgatgacgc agaagatttg ctcggtgttg ttcacgttat cgacctgcta
    28921 caacagtcac tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca gccgctggtg
    28981 ttcccggaaa ccttgccgtt gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac
    29041 tttgcttttg tagtggatga gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc
    29101 actgaaacca ttgccggtaa cttaccgaac gaagtggaag agatcgacgc ccgccatggt
    29161 attcagaaga atgcggacgg ttcctggacg gcgaatggtc atatgccgct ggaagatctg
    29221 gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat tgccgggctg
    29281 ttgatggaat atttacagcg tattccaaaa cctggcgaag aagttcaggt gggggattat
    29341 ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aggtgcagat tattccgctg
    29401 cataaggatg gcgagttgga gtacgaggtg tgatggtatc ggtcatttta cccgatagcc
    29461 gaaaggaata agatgaggga accgcgccag cggcgattta ccgccgggag cccgggttgc
    29521 cagggtggtg gcgattgagc caccctggca cgttcaccgg cttgagcgtt ccatagtagt
    29581 gaggaacatc aagtgaacgg aacagccttc gatcttacat attcagaata taatggtgcc
    29641 gccgcgaacg cctgatgcga cgctttacgc gtcttatcag gcctacagga caggtcacct
    29701 ggtaacctac atcttctcca gcaacttctt cacatctttg ccattgcggg aatctttatt
    29761 ccgctcggcc cagtcattca ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa
    29821 ctgatccact tgcagactgt aattgagttg ctgccagttg ccataaaccc gcagcggtac
    29881 tggcgtttct ttgaggaaat caatcagttt gctttccccg ttccagccac ccacgacccg
    29941 aatatcaaac tgggtgtcgc aggtttgatc tgccagattc aacatgcctt cccctgtcag
    30001 cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt
    30061 caaatcggtg gtaaagcggt caagacgcgt tacgttatcg aagttttcag cggccttcac
    30121 atcaccacca ttacgttcta ccgcttgctg aatcatctgc tggaagttca tcccttccat
    30181 gcgcgtgtcg gtcatttcga catgtgcctg tccttgccag ttgtggcgga atgcgtcggc
    30241 atctatgtca gcaccggaga aatcaccagc cagtgacatt tttccggtca acgaaatcgg
    30301 atagttaaac gccttcagaa tggtaccaat ctcaacgttt tccagccgtg gctggaagtt
    30361 tatccgcgga tttattgatg tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt
    30421 aagcttgccc tgcagttgag taatttccag caaacccgac ttgttggtca tttgcgtggc
    30481 aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca aaatatcagc
    30541 cgtaaagcct tgcagtccct gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg
    30601 cggcaatgtt gattggctct gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc
    30661 attaagcggg atgaggttgt caagattcag ttgcgggaat tgcagtctca gctgccattc
    30721 aggtttctct gtcagcgtga cctgtgcttg cccgctcagc gtgctgtcat tggcggtcaa
    30781 gctgatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct ggaaactccc
    30841 ctgcccctga ataccttgtt ttggtaaatc tgcaccctgc aactgccagt taatttgttc
    30901 aatagccgcc gttaaatcat gcggataatc agaagcatct accgtaccgt taagggatat
    30961 cgtgagatcg cgctgatcgc gattaacccg cccggagaac tcaaatgagc cacgatgttg
    31021 gggatcttgt tccatttgca ggcggatatt gcggattgtc acctgctcgt catcttcatg
    31081 ctggaacacc agcacgctgt ccgccacctt aagactggat atatcaaacg accatccgcg
    31141 atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt cttcactgcg
    31201 caccgcttcc gtctgcggcg tcagttggat cactgcccct tttagcatca cctgcttaac
    31261 gctcagttga tgactcagta gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg
    31321 aaccagtggc tggcttgcgc cctgggcggt gagagacatt cgcccggaga ggatactaag
    31381 ctgcggccag acgtgccaac gcagtggccc gtcgagctgc aattgataac cgctacgtgc
    31441 agcaacttgc ttgaccatat agtcgcggaa atcattcgga ttcaccagca acactaacgc
    31501 agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat
    31561 ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg cgccctgctg
    31621 gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg
    31681 ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca gcttaccgga
    31741 gttgtagaac tccagcacaa tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac
    31801 gtgcaccatc agccccagac gcgccaaaga ggaacgcccg tccagccagc ccaccagatc
    31861 ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agctctcctg ggtgaagata
    31921 gaacgcctcg ccctcgtcga gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct
    31981 cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg
    32041 aaatttattg cccaggcgta catccaccgt cgcgccgtta atacgctcca ctggcggacg
    32101 tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt cacacagacg
    32161 catggcattt tctcctttcg cgcatcactc ccttaacgcc aattacgtca agggcatacc
    32221 aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga cttattttcg
    32281 ctttcaatat atcgatcgcg atgcggtttt tcccgccgcg cggcacgata atgtccgcat
    32341 attgtttaga aggctcaatg aattgcagga acatcgggcg cacggttttt tgatattgcg
    32401 ccatcacgga atccattgaa cgcccacgct cgttaacgtc acgcttgatg cggcgcatca
    32461 ggcagatatc cagcggggta tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg
    32521 catccgtcag caacaaaatg ccttcgagaa tgatgacctt cttcggctca accgtcaccg
    32581 tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc
    32641 gtttcaacgc ttgtaaatgc tcaagcaaca ggctgtgatc catcgcgctg ggatggtcgt
    32701 agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa tagcagtctt
    32761 cgggaattac gccgatgtgt tcatcaccga cttgctcacg taattcacga taaagggtac
    32821 tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg acgcactgat
    32881 gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga agggggcgac
    32941 gaagcgccaa acgcggcaat tatagggatt tcatccgcct gataccagtc gaatagcgct
    33001 gccgcgcgct cagagttaat tgttgacgaa gaattcccgg tggcaaatta cgttgatcag
    33061 ttttatacaa ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct ttacgtcact
    33121 ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact agcataaaca cagaaacgaa
    33181 tactggcgac ctggtcttgc ggataaagcg gtaatgagca aacaatcaca gcatgtatta
    33241 attgccctgc cccacccgct gcttcacctg gtcagtttag gtttagtctc gtttatcttt
    33301 acccttttct cgcttgagct ttcgcagttt ggcacccaac tcgccccact gtggttcccg
    33361 acgtccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc gggaattgcg
    33421 ctgagctgct cgctgggaaa tatcgccgca tccatcctgc ttttttccac cagctcgctg
    33481 aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt gctgctacgt
    33541 aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg tctggcattc
    33601 ggcagcgcca ttgttccgcc tctgctaggg ggtgttctgg ttgtcctgct gacgcccgga
    33661 gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca
    33721 ctggtgccgc tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccacgg
    33781 ttgctttttg agtcgctgct cacgttagcc atcacactga cgttaagctg gctttcgatg
    33841 ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgc
    33901 ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt gtcgctgatg
    33961 atggccgcgg atccctccct gcttgctacg ccgcgtacat acctgatgag ccatatgccg
    34021 tggctaccgt ttttgctgat cctgctgccc gccaacatca tgacgatggt gatgtatgcc
    34081 tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gttttcggaa cgccatggaa
    34141 tattccgcta tcggtatggc gttagtgggc accgagggac aatggctgca aaccaacaaa
    34201 gcgctctgcc agtttctcgg gtacagtcag gaagagctgc gcggactcac ctttcagcaa
    34261 ctgacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct gataagcggt
    34321 gaaataaaca cctattcaat ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg
    34381 gcgttgcttg ccgtctcact ggtgcgccac acggatggca cgccgctcta ttttatcgct
    34441 cagattgaag acattaacga gctaaaacgc accgaacagg tgaatcagca actgatggag
    34501 cgcatcacgc tggctaacga agcgggcggg attggcatct gggagtggga gttgaagccg
    34561 aatattttta gctgggataa gcggatgttc gagctgtatg aaattcctcc acatatcaaa
    34621 ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa
    34681 gtgattcgtt gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg
    34741 gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac
    34801 gcctgctcgg cattaatatg gatatgactg aggttaaaca gcttaacgag gcattgtttc
    34861 aggaaaaaga gcgcctgcac ataacgctgg actccatcgg cgaagccgtg gtctgtattg
    34921 atatggcaat gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc
    34981 aggaagaagc attaggtgtt ccgctcctga cggtgttgca tattaccttt ggcgacaatg
    35041 gtcccctgat ggagaacatt tacagtgccg acacctcacg ttccgcgatt gaacaagatg
    35101 tggtgttgca ctgccggagc ggcggcagct acgacgtgca ttacagtatt acgccgttaa
    35161 gtactctgga cggcagcaat attggttcgg ttctggtgat tcaggacgtt accgaatcac
    35221 gcaaaatgct gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca
    35281 atcgcgccag ttttgagaag caactgcgta tgctgctgca aacggtaaac agtacacatc
    35341 agcgacatgc cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg
    35401 ggcatgcggc gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc
    35461 gttccagcga cgtgctggcg cgactcggtg gggatgaatt tggtctgctg ctaccagact
    35521 gtaatgttga aagcgcgcgt tttatcgcta cacgcattat cagtgccgtg aatgactatc
    35581 actttatatg ggaaggccgt gtgcatcggg taggtgccag tgccgggatt accttgattg
    35641 atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct
    35701 ccaaaaatgg tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata
    35761 gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga
    35821 tgatgatggc ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga
    35881 tttcgcttaa actctggagt tgcgaaggcg agattattga tgaacaaaaa tttcgtcgta
    35941 gcttcagcga tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc
    36001 agctggccgc aaaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg
    36061 ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc
    36121 taccaccacg gttattacat ctgattattc cggcagacgc gattttagat cacgcagaaa
    36181 gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc
    36241 tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt
    36301 tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc
    36361 acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg
    36421 atacgctttc tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac
    36481 cgctggattt gctggtgaat agcagttatt tcgcgattaa ctgagggagc gtttgcccat
    36541 tgcctgatgc gacgcatcag gcctacaatt cgtccgttcc gtctggttgc cagccttccg
    36601 tataccagat atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc
    36661 ggatttgtgc cggtgtcatc cccggaaatc gctgtttaat cagataatca tccggcagaa
    36721 aaacatcttt cgcctgccag ccacgcaaag cgaaataatt cgccgtccag cgcccgatac
    36781 ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta
    36841 agctgccctc cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca
    36901 tgcctaacgc ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc
    36961 agatatactc cggaaaatca tccagccgtt cgccataaag ctgtacaact ttggcagtca
    37021 attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa
    37081 aagcatcaat acagccgggt aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg
    37141 caccgttaac aatctgcggg ttacattgca gatcaaacag gcggctcatt ttcgccagac
    37201 actcagcggc aacgggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta
    37261 tatccggaat agcagtcacc acgccgcgat attcgcccac cgccagacta cgggcataat
    37321 aatcctcagc gaccgtttcc acaccactca ctgcacgggc ggcgagaaat cccaacatcc
    37381 acgaccagtc atacggcggc tgccagttca gggtatacat cgcatctcct tattcatccg
    37441 ctttcagcat aaacgttatt cagacgctac gctttgcttt catattccgg ttgtcgcgac
    37501 agcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattaa ttcgtgttta
    37561 ttggttttga ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc
    37621 aattactgaa aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc
    37681 gtgaatcggt aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa
    37741 cgcaggcgct gttacgtcgg gcgattcgtt ataaccgcga agaagatatc gatgttacgg
    37801 cgaaaagcgt gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag
    37861 tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg
    37921 tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg
    37981 cgcagctgcc agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc
    38041 tgggcggcga tgaagcaaac gcccaggcgc aagggattct ggaacgcgcg gcgaagcgtg
    38101 ccggattcag ggacgtggta ttccagtacg agccggtcgc ggctgggctg gattacgaag
    38161 ccaccttgca ggaagaaaaa cgggtgctgg tggtggatat cggcggtggt acgactgact
    38221 gttcattgct gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc
    38281 tgggtcacag tggttgccgt attggcggta acgatctgga catcgcactg gcgtttaaaa
    38341 acctgatgcc gttactgggc atgggtggcg aaaccgaaaa aggcatcgcc ctgccgattc
    38401 tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta
    38461 gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg
    38521 ccctgctaca gaaagtctgg cgtgagcgtt taagctatcg cctggtgcgt agcgcagaag
    38581 agtgcaaaat tgctctttca agcgtagcgg aaacccgcgc ctcactgccg tttatcagcg
    38641 atgaactggc tacgctgatt agccagcaag ggctggaaag cgccctcagc cagccactgg
    38701 cgcggattct ggaacaggtg caactggccc tggataacgc ccaggaaaaa ccggacgtta
    38761 tctacctgac cggcggtagc gcccgttcgc cgctgattaa aaaagcgctg gcagaacagt
    38821 tgccgggaat tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac
    38881 gctgggcgga agtggtgttt cgttaaaaga aaatggctca gcagcggctt aaatctgcct
    38941 acgcctgagc cagtttttct gccgcctgac gtaaaggttc catcaacggc atggtccgtg
    39001 ctggagcatg aaggcggaat cgctgcaact ctgtcgcgcc gacagtcaac gtcgattcca
    39061 gccgatgtcg tttggcggtc attttcgcca ttaccgcttg tctgtctgct ggcgttatcg
    39121 cttcattcgg cctgaacaca aaacggcgtt cacagctcgc tttccagtcg ataaccgcct
    39181 gggtcagatg gtcgccaaac cctttcactt gcttaacgcc acgacgggta acatctgcgg
    39241 ctgtttcaat accaaaagac cgtaacgccg ctttacgcgc agggccaacg ccgggaatag
    39301 aggcaacatc aataaaaaat ccctccagaa acttctgctt ctgccgttcc cttgcagtgt
    39361 catgaagtgc tgccagagcg cgtttctctt cttccggtaa cccgagaatt tcgtccttca
    39421 ttttttcgag catcgcccgt ttggcgataa aaccttccag cccgcccaac tgttggatct
    39481 ggctgactaa atgctcatag tccattttcg cgcgattaaa tgcctcacgg cgttgctgga
    39541 tttctgcttt gtacgctttg cttgtcagac tgccaataat ccagatagcc gacagcacag
    39601 gaaccaaaat aatataacgc ggttctgcct gaaggccgca aagcaacagc gataacgctg
    39661 acagtgcgat ctcaatcaga atgatgtatt cgcgccgtaa caggcctaaa ggaagcggcc
    39721 tgccagtcgg ttggaaatga tcgggtaatg gcaattgcaa tgctggcggt gctactgacg
    39781 ccatcaccat cgcccagact ttcgccagca caaaatcacc gccggtggta atgacctctt
    39841 cgccgagatc aataaaatag ataacgcctt gattatccag cgcacaccac gggcagtcgg
    39901 tcaaatgagc gggataaaca tgcattgccg aaacgggaca tttctttaac tgttggcgca
    39961 gagaatcaag tgccgctacc cacgctttag ccgtcggacg cccggttgcc acgccacttt
    40021 ccgtgaatgc ctgctgaaac atggcttcaa catcgcccgg taacatcgat agcggaatcg
    40081 atcgtggcgg cggttttaaa ccacgtcgct gattatctga cgcgtaggca taacggaaat
    40141 gggtaatatc cgtctccagc gcattacccg catcagagat aagcggcaca ccagaataag
    40201 gatgccggcc accaaacaag acgtgaaaaa tcagcaacgc aaggccaaaa ttatcgtgat
    40261 tttctgtgcg ttcaaagccg acaaatgacg acaaggtttg tagctctggc ggcgtaaaat
    40321 gcgacacgcc gacttcgcat aaatgcagtg tgccattggc gttaatctga aaggagtcgc
    40381 tatcaatcag taccactttg ctgtcgcgac ctaccataaa actgttctgg tttacgtcac
    40441 ccacgacgtg cccgtgctcg tgaaccgtag caaaagatga agcaatattg cgcgcaacat
    40501 agagtagaaa atcccacgca caatgagggt aacgttgacg acgatgtgcc gggctataga
    40561 tcatatgaat cggttcttta ccagaaactt ttggcatcat aaaaccgata acttttccgc
    40621 ctcgtccacc gtgaagcgtt gcctgcggcc aggcgacata attcaacaat tgcgcgtcag
    40681 ctgtcgcagc cataaaggca agtttgtcct gttttaaggc gggtggcggc gtgtgataaa
    40741 tcttggcgac gctatcgaca aactcattga tatcataaac cgcgccttca ccgcctttgc
    40801 ccagttcacg gcctggcgta acgcactcac cagtagcagt atataaagtg ggtttcatgg
    40861 taaccacagc gccagtgcca acgttttatc atcatccgta cgctcgttta ccgccgggct
    40921 ggacaaaaac tgctttaaca attcaggtaa taaatcgagt tgttcctgcg ttgctgacgc
    40981 cagtccattg aaaaacgggg taaagaaagg tacatgagga gaattatcca gcatatttaa
    41041 cgccagtcgc tggataccat ccgtaaatgc tgcaacttta tgcacacgct cagtgctggt
    41101 gaaagtttcc agtctggaaa cggcatcttc atcggtaatg aagtgcgtca tattggcata
    41161 ttcacccacc atcggcgtaa gcggcaattg cagaccgtga ccaaaatcca ccaccacgcc
    41221 gccatcgcca atctgcataa tcaaggtgcc attagccgat gaaatcaatc ccagaaacgt
    41281 gcaggcaaaa tcgcgaactg ctaattcttt ggcttccgcc tcggcaaaaa gtcgctgacg
    41341 aacggtcagc acaatatccg tggcgaggat atcattaagc cccaattccc caccctgcac
    41401 tttttgcgac atataagcca tcgcttcatt gacggcgagc gtcgcgcctt ctcctccctg
    41461 cgaaacgcta cctgcgccat cagcaaggaa catcaccagt aatggctgct ggtcatttag
    41521 ccaggcaacc tgcatctggc aggcatcctg gcaaggcaag tccgcgctga tgtgcgatgt
    41581 tccaacagcg gatgcataaa ccagacgcca gctcacactg acgtccagcc ttttggcgct
    41641 tccagcacaa cttccgttcc cggcgtggag cgggaaaccg aacgcagcga actggataac
    41701 cagctaaaca gttcacggaa ttgtaatcct tgcagaggca aaggctgacg aacgctgatt
    41761 tgtgccagag tcttcatatc cgccccctga acaccgatgg aaaagaaagc aaacctttta
    41821 tcttcttccc cccgaaacac tttgttggct gcagcctgcc actcatcggt tggtgcacca
    41881 tcggtaatca ggaaaatcca cgggcgataa tacgaaatac catttgcacg atactcacgt
    41941 ttccgctcct ctaccatatc cagggctttg gtaatggccg cacccattgg tgtatcgccc
    42001 tgggcaaaca ggatgggcgg aaaaaaatta gcggcgctgg taaagggttg ttccacatgc
    42061 accgggccga acgtcacaat gccgagttcc actcttttta aagccagcgg atcggcaagc
    42121 agttcatcac gaaaggtcac caatccggcg ttaagttcat taataggtct gccgctcatc
    42181 gagccagaaa catccagcag caaaatgcag gggcaacgtg gttccgggtt actggcaaaa
    42241 tcgctggtgg caaacgtgat ttgttcgctc ataagaaatc ctttcttaga gaattatcga
    42301 ggtccggtat cataccgact gcaataatga aataagagca aacgaagaaa ctttcagaat
    42361 taggcccggc ggcgcagaaa attttgctat tcgcctcgca agaacggaga gccgtcgatt
    42421 gacactaagg gcggagtgac ataatttcag gagtgagggt tagggagagg tttccccctc
    42481 cccctggtgt tcttagtaag cctggaagct aatcactaag agtatcacca gtatgatgac
    42541 gtgcttcatc ataacccttt ccttattaaa agccctcttc tccgggagag gctttcccgt
    42601 ttcagcgtcc cgctgaaatc atcggcttac ctcctttcgc cctgaatgca gtctatcgct
    42661 aacgcgtgaa taaaatagtt tcctctgtga ttattgcgta acgactcgca aagaatattt
    42721 gctggcagga tcgcagacta caaagcctgc ggattgacaa tcttatcgtg aaggcatact
    42781 ttcaggagtg agggtagagc ggggtttccc ccgccctggt agtcttagta agcggggaag
    42841 cttatgacta agagcaccac gatgatgagt agcttcatca tgaccctttc cttatttaag
    42901 gccccttcct cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct
    42961 cctttcgcca tgcacttagt ctatcgctga cttgcagata aaatagtttc cagtgtgatt
    43021 attgcgtaac gtctcgcaaa gaatttttgc tggcaggatc gcagacggca aagcctgcgg
    43081 attgacaatc ttatcgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc
    43141 gccctggtag tcttagtatg ctgggtagct aatcactaag agcaccacga tgatgagtag
    43201 cttcatcatg accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca
    43261 gcatccggct gaaatctgcg gcttacctcc tttcgccctg tatgcagtct atcgctaacg
    43321 cgtagataaa atagtttcct gtgttattac tggatgcgtg ctcgcaaatg tgcccgccat
    43381 tcagacgatt cccgatagtg tttcataatt cctccatttt tctcccttat tggctggcta
    43441 cactagtatc attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt
    43501 ataaatcccg ttgggtaatc gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt
    43561 tctggcaagg ccgcaatgac tcccggagtg cagccccagg ggcgacgaaa caagcgcagc
    43621 aatcgccagc gggtggtcgc cgtggtatgc gttccggccc attagccccg gttcaggcgg
    43681 cgaccgccgt agaacaggca gttccgcgtt acctcaccgg gcttggcacc attatcgccg
    43741 ctaatactgt tacggtgcgc agccgcgtgg acggtcaact gatggcgtta catttccagg
    43801 aaggccagca ggtcaaagca ggcgatttac tggcagaaat tgaccccagc cagttcaaag
    43861 ttgcattagc acaaacccag ggccaactgg caaaagataa agccacgctt gctaacgccc
    43921 gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc
    43981 tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa
    44041 gcgtcgccag cgcgcagttg caactcgact ggagccgcat caccgcacca gtcgatggtc
    44101 gcgttggtct caagcaggtt gatgttggta accaaatctc cagtggtgat accaccggaa
    44161 ttgtggtgat cacccagacg catcctatcg atttagtctt taccctgccg gaaagcgata
    44221 tcgctactgt agtgcaggcg caaaaagccg gaaaaccgct ggtggtagaa gcctgggatc
    44281 gcaccaattc gaagaaatta agtgaaggca cgctgttaag cctcgataac caaatcgatg
    44341 ccacaaccgg tacgattaaa gtgaaagcac gctttaataa tcaggatgat gcgctgtttc
    44401 ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc
    44461 caacagccgc cctgcaaatg ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa
    44521 acaaggtcag caaacatctg gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc
    44581 gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag
    44641 gggcgaaagt ggaagtggtg gaagcccaga gcgccaccac tccggaagag aaagccacca
    44701 gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag gtgttacccc cgagcagcac
    44761 aggcggcccg tcgcgcctgt ttattatgcg tcctgtctcc accacgctgc tgatggtggc
    44821 gatcttactc gccgggatta tcggttatcg cgccctgccc gtttcggcgc tgccggaagt
    44881 ggactatccg accattcagg tggtcacgct ctacccaggt gccagcccgg atgtcatgac
    44941 ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag atgtctggcc tgaaacagat
    45001 gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg cagttccagc taacattacc
    45061 gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac gctgcgacca acttgttgcc
    45121 gagcgatctg cctaacccgc cggtttacag caaagtgaac ccggcagatc cgccgatcat
    45181 gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa gtggaagata tggtggaaac
    45241 ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg
    45301 tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg attgccgccc tcggcctgac
    45361 cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt aactcggcaa aaggtagcct
    45421 cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga
    45481 gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca attcgtctgg gcgatgtcgc
    45541 aactgtagag caaggtgcag aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc
    45601 cattgtgatg aatgttcagc gccagcccgg tgctaacatt atctccaccg ccgacagcat
    45661 tcggcagatg ctgccacagc tcactgagag tctgccgaaa tcggtgaagg tgacagtgct
    45721 ttccgatcgc accaccaata tccgcgcatc cgtcgatgat actcagtttg aattgatgat
    45781 ggctatcgcg ctggtagtca tgattatcta cctgtttttg cgcaatattc cggcgaccat
    45841 cattcccggt gttgctgtac cgctgtcgtt aatcggcact ttcgcggtta tggtgtttct
    45901 cgatttttca atcaataacc tgacactgat ggcgttaact atcgccaccg gattcgtggt
    45961 cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc
    46021 gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt accattatct cgctgacctt
    46081 ctcactgatt gcggtgttga tcccactgct gtttatgggc gatatcgtcg ggcgactgtt
    46141 ccgcgaattt gctattacgc tggcggtagc gattttgatc tcagcggtgg tgtcgctgac
    46201 cctgacaccg atgatgtgcg cgcggatgct cagccaggag tcgttgcgta aacagaaccg
    46261 cttctcccgt gcctcggaaa aaatgttcga caggataatc gccgcctatg gtcgtggact
    46321 ggcgaaggtg ctgaatcatc cgtggctgac cttaagcgtg gcgctcagca cgctgctgct
    46381 tagcgttctc ttatgggtgt tcattccgaa aggtttcttc ccggtacagg acaacggcat
    46441 tattcagggc actttgcagg caccgcaatc cagctccttt gccaatatgg cccagcgaca
    46501 acgccaggtc gcggacgtga ttttgcagga tccggcagtg caaagcctga cctcatttgt
    46561 tggcgttgat ggcactaacc cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc
    46621 gctggatgaa cgtgatgacc gagtgcaaaa agtcattgcc cgtctgcaaa cggcggtgga
    46681 taaagtgccg ggcgtcgatc tcttcctgca accaacgcag gatctgacta ttgatactca
    46741 ggtcagccgc acccagtacc agtttacctt gcaggccacc tcactggatg cgctcagtac
    46801 ctgggtgcca cagttgatgg aaaaactcca gcaactgcca cagctttctg atgtctccag
    46861 cgactggcag gacaaagggc tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg
    46921 tctggggatc agcatggcgg atgtcgataa cgccctgtac aacgcgtttg gtcagcgact
    46981 gatttccact atttatactc aggctaacca gtatcgcgta gtgctggagc acaacaccga
    47041 aattaccccg ggcctcgcgg cgctggatac cattcgcctg accagtagcg acggcggcgt
    47101 ggtgccgtta agctcaattg ccaaagttga acagcgtttt gctccgcttt ccatcaacca
    47161 tctggatcag ttcccggtaa cgaccatctc ctttaacgtg ccggataact attcgctggg
    47221 cgatgcggtg caggcgatta tggacaccga aaagacgctg aatctgccgg tggatatcac
    47281 cacgcaattt cagggcagca ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct
    47341 gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc attctgtacg agagctttat
    47401 tcacccgctc accattctct cgacgctacc caccgcaggg gtcggcgcgc tgctggcatt
    47461 aatgattgcc ggtagcgaac tggatgtgat tgcgattatc ggcattattt tgctgatcgg
    47521 tattgtgaag aagaacgcca tcatgatgat cgacttcgcg ctggctgctg agcgcgagca
    47581 aggcatgtcg ccgcgcgatg caatctacca ggcttgtctg ttgcgttttc gtccgatcct
    47641 gatgaccacc ctggcggctc tgcttggcgc gctgccgctg atgttgagta ccggggtcgg
    47701 cgcggaactg cgtcgtccgt taggtatcgg catggtcggc ggtctgattg tcagccaggt
    47761 gctgacgctg ttcaccacgc cggtgattta tctgctgttc gaccgcctgg cattgtggac
    47821 caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg aagttttttg ccctcttcat
    47881 ttaccgcccg gtggcgacga ttttactgtc ggtcgccatt accctgtgcg gcatactggg
    47941 cttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat tttccggtga ttatggtcag
    48001 cgcctcgctg cccggtgcgt caccagaaac aatggcgtct tccgttgcca cgccgctgga
    48061 gcgctcactt gggcgcattg ccggagtcag tgaaatgacc tccagcagtt cgctcggcag
    48121 cacgcgtatt attttgcagt ttgattttga ccgggatatc aacggcgcgg cgcgcgatgt
    48181 gcaggcggcg atcaacgctg cacaaagttt gctgcccagc gggatgccca gccgtccgac
    48241 ctatcgcaaa gctaacccgt cggatgcgcc aattatgatc ctcacgctga catccgatac
    48301 ttattcgcag ggtgaactgt acgattttgc ctcgacgcag ctggctccga cgatttcgca
    48361 aatcgacggt gtcggtgatg tcgatgttgg cggcagctcg ctgcccgccg tacgcgtcgg
    48421 gctgaatccg caggcgctgt ttaatcaggg cgtctcgctg gacgacgtac gcaccgccat
    48481 cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa gatggcactc accgctggca
    48541 gatccagacc aatgatgagc taaaaaccgc cgccgaatat cagccgttga ttattcatta
    48601 caacaacggc ggcgcggttc gtctgggcga tgtggcgacg gtgaccgact cggtgcagga
    48661 tgtgcgcaac gccgggatga ccaacgccaa accggctatt ttgctgatga tccgcaagct
    48721 gccggaagcc aatattatcc agacggtaga cagcatccgg gcaaaattac cggagttgca
    48781 ggaaaccatt ccggcggcga ttgatctgca aattgctcag gatcgctccc ccaccattcg
    48841 cgcctcgctg gaagaagtcg agcaaacgct gattatctcg gtggcgctgg tgattctggt
    48901 ggtgttttta ttcctgcgct cgggtcgcgc cactattatt cccgccgttg cggtgccggt
    48961 ttcgctgatt ggtacgtttg cggcgatgta cctgtgcgga ttcagtctca ataacctttc
    49021 gttaatggcg ctcaccatcg ctactggttt cgtggtggat gacgccatcg tggtgctgga
    49081 aaacattgcc cgtcatctgg aagcggggat gaaaccgcta caggccgcgc tacaaggtac
    49141 tcgcgaagtc ggctttacgg tgctatcgat gagtctgtca ctggtggcgg tgttcctgcc
    49201 gctgctgttg atgggcggat tgccgggccg actgttacgc gaatttgccg tgacgctttc
    49261 tgtcgccatt gttatatcgt tgctggtttc tctgacatta acgccaatga tgtgtggctg
    49321 gatgctgaaa gccagcaagc cgcgtgagca aaagcgactg cgtggttttg gccgcatgtt
    49381 ggtagccctg caacaaggct acggcaagtc gctgaaatgg gtgctcaatc atacccgtct
    49441 ggtgggtgtg gtgctgcttg gcaccattgc gctgaatatc tggctgtata tctcgatccc
    49501 gaaaaccttc ttcccggagc aggacaccgg cgtgctgatg ggcgggatac aggcggatca
    49561 gagcatttcg tttcaggcga tgcgcggcaa attgcaggat ttcatgaaaa ttatccgtga
    49621 cgatccggca gtggataatg tcaccggctt taccggcggt tcacgagtta acagtgggat
    49681 gatgtttatc accctcaagc cacgcgacga acgcagcgaa acggcgcagc aaattatcga
    49741 ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat ctgttcctga tggcggtaca
    49801 ggatattcgc gttggtgggc gtcagtcgaa cgccagctac cagtacacgt tgttatccga
    49861 cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc
    49921 ggaactggcg gacgtgaact ccgatcagca ggataacggc gcggagatga atctggttta
    49981 cgaccgtgac accatggcac ggctgggaat cgacgtacaa gccgccaaca gtctgttaaa
    50041 taacgccttc ggtcagcggc aaatctcgac catttaccag ccgatgaacc agtataaagt
    50101 ggtgatggaa gtggatccgc gctataccca ggacatcagt gcgctggaaa aaatgttcgt
    50161 tatcaataac gaaggcaaag cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa
    50221 tgccccacta tcggtgaatc atcagggatt atcggcggcc tcgaccattt cgttcaacct
    50281 gccgaccgga aaatcgctct cggacgccag tgcggcgatc gatcgcgcaa tgacccagct
    50341 tggtgtgcct tcgacggtgc gcggcagttt tgccggaaca gcgcaggtgt tccaggagac
    50401 gatgaactca caggtgatcc tgattatcgc cgccatcgcc acggtgtata tcgtgctggg
    50461 tatcctttac gagagttatg tgcacccgct gacgattctc tccaccctgc cctcggcggg
    50521 tgttggagcg ctgctggcgc tggagctgtt caatgccccg ttcagcctaa tcgccctgat
    50581 agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc
    50641 gcttgaagcc caacggcacg gtaacctgac gccgcaggaa gctattttcc aggcctgtct
    50701 gctgcgtttt cgcccgatta tgatgactac cctggcggcg ctgtttggcg cgctgccgct
    50761 ggtgttgtcg ggcggcgacg gctcggagct gcggcaaccc ctggggatca ccattgtcgg
    50821 cggactggta atgagccagc tccttacgct gtataccacg ccggtggtgt atctcttttt
    50881 cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac
    50941 agatcttccc gacagcaccc gttggcaatt gtggattgtg gctttcggct tctttatgca
    51001 gtcgctggac accaccatcg taaacaccgc ccttccctca atggcgcaaa gcctcgggga
    51061 aagtccgttg catatgcaca tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct
    51121 gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc aatattttct ttaccgccat
    51181 cgtgctgttt actctcggtt cactgttttg cgcgctttcc ggcacgctga acgaactgtt
    51241 gctggcacgc gcgttacagg gcgttggcgg cgcgatgatg gtgccggtcg gcagattgac
    51301 ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg atgacctttg tcacgttacc
    51361 cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt ctgctggtgg agtacgcgtc
    51421 gtggcactgg atctttttga tcaacattcc ggtggggatt atcggtgcga tcgccacatt
    51481 gatgttaatg ccgaactaca ccatgcagac gcggcgcttt gatctttccg gctttttatt
    51541 gctggcggtt ggcatggcgg tattaaccct ggcgctggac ggcagtaaag gtacaggttt
    51601 atcgccgctg gcgattgctg gcctggtcgc agttggcgtg gtggcactgg tgctttatct
    51661 gctgcacgcc agaaataaca accgcgccct gttcagtctg aaactgttcc gcactcgtac
    51721 cttttcgctg ggcctggcgg ggagctttgc cggacgtatt ggcagtggca tgttgccctt
    51781 tatgacaccg gttttcctgc aaattggcct cggtttctcg ccgtttcatg ccggactgat
    51841 gatgatcccg atggtgcttg gcagcatggg aatgaagcga attgtggttc aggtggtgaa
    51901 tcgctttggt tatcgtcggg tactggtagc gaccacgctg ggtctgtcgc tggtcaccct
    51961 gttgtttatg actaccgctc tgttgggctg gtactacgtt ttgccgttcg tcctgttttt
    52021 acaaggaatg gtcaactcaa cgcgtttctc ctccatgaac accctgacgc tgaaagatct
    52081 cccggacaat ctggcgagca gcggaaacag cctgctgtcg atgattatgc aattgtcgat
    52141 gagtatcggc gtcaccatcg ccgggctgtt gctgggactt tttggttcgc agcatgtcag
    52201 cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc tggcttagca tggcgttgat
    52261 catcgccctt ccggcgttca tctttgccag agtgccgaac gatacgcatc aaaatgtagc
    52321 tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc gacccggtat taccggcaaa
    52381 ctgtttctgg cgattttcgc cacctgcatt gtcttgctga tcagtatgca ctgggcggtg
    52441 cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc atggtaatga acaacgatta
    52501 caactgttaa gtgatgcgct tggcgagcag tatgcgcagc atggcaactg gcgcttcctg
    52561 cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat ttgaacacga taattcggaa
    52621 gataaacccg gcccgggtat gccaccgcac ggctggcgta cccagttctg ggtggttgat
    52681 caaaacaaca aagtgctggt tggtccgcga gcgccgattc cacctgacgg tacacggcga
    52741 cccattctgg tcaacggtgc ggaagttggc gcggtgatcg cctcccccgt tgagcggtta
    52801 acgcgcaata ctgatatcaa tttcgataaa caacagcggc aaaccagctg gctgatcgtc
    52861 gccctggcaa cgttactcgc ggcgctcgcc acctttctgc tggcgcgcgg tttgctggca
    52921 ccggtaaaac gacttgtcga tggcacgcac aaactggcgg cgggcgattt cactacccgc
    52981 gtaacgccca ccagtgaaga tgaactgggc aaactggcgc aagacttcaa ccagcttgcc
    53041 agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta tggccgatat ttcacacgaa
    53101 ctgcgtacgc cattagcggt gctgcgcggt gaactggaag ccattcaaga tggcgtgcgc
    53161 aaattcacgc cggagacggt ggcgtcttta caggcggaag tcggtacact gactaaactg
    53221 gttgacgatc ttcatcagtt gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca
    53281 ccggtagatt tgatcccact gctggaagtg gcgggcggcg catttcgcga acgatttgcc
    53341 agccgtggcc tgaaactgca gttttccctg ccagacagta ttaccgtatt tggcgatcgc
    53401 gaccgtttaa tgcagttatt caataactta ctcgaaaaca gcctgcgcta cactgatagc
    53461 ggcggcagcc tgaaaatctc tgccgagcag cacgacaaaa cggtgcgcct gacctttgcc
    53521 gacagtgcgc caggtgtcag tgacgagcag ctacaaaaat tatttgaacg tttttatcgc
    53581 accgaaggtt cccgcaaccg cgccagcggt ggttccgggc tggggctggc gatttgcctg
    53641 aacattgttg aagcacataa tggccgcatt attgctgccc attcgccttt tggcggggta
    53701 agcattacag tagagttacc gctggaacgg gatttacaga gagaagtatg accgagttac
    53761 caatcgacga aaacacaccg cgtattttga tcgtggaaga tgaaccgaag ctggggcagt
    53821 tgctcattga ttatctgcgt gctgcgagct atgcgccgac gcttatcagc cacggcgatc
    53881 aggtactgcc gtatgtgcgc cagacaccac cggatctgat cctgttagat ctgatgctcc
    53941 ctggcaccga tggcctgacg ctgtgccggg aaattcgtcg tttttctgac attccgatcg
    54001 tgatggtgac ggcaaaaatc gaagagatcg atcgcctgct ggggctggag attggcgcag
    54061 atgattatat ctgtaagccg tacagcccac gggaagtggt agcgcgcgtc aaaaccattt
    54121 tgcgccgttg caaaccgcag cgcgagttgc agcaacagga tgctgaaagc ccgttgatta
    54181 tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat gcttgacctg acgcctgcgg
    54241 aatttcgtct gctgaaaacg ctctctcatg aaccaggaaa agtgttctcc cgcgagcatt
    54301 tgctcaatca tctttatgac gactaccgcg tagtaaccga ccgcaccatc gacagccaca
    54361 ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga acagtcattt atccgcgccg
    54421 tttacggcgt tggttaccgc tgggaagccg acgcctgccg catcgtttag ttttagcgac
    54481 atgactttgt tagccggaga tggcgttcag accagatccg gcaacattat cccacgcatg
    54541 gtcagcaaac tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta
    54601 atcggtaaac agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt
    54661 atggctggtt ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa
    54721 gcgggcaatg gtgagactat cctcaccagc gagctgtata cctcaaaagc ctctgcggaa
    54781 aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg
    54841 gcaagtaacg gcaaattcta tttcaatctg aaagccgcta atcatcaaat tatcggctcc
    54901 agccagatgt acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat
    54961 ggcacaagcc agacggtgaa agacaatacg taattacgat gccgggcgtg ggttgttggc
    55021 gtccggcttt atatccaaat aacgcttagt tattaataat taagagtaac cgccgccatt
    55081 aaaatagtgg gcactgacga gcactcgttg tgctttcttt gtttaccatc ccatacttat
    55141 cctcctgaac atgttcatgt gccaaaatat cttctaatac tttcgtgacg ctatccggtt
    55201 tatgacatac actgatgtaa gatttttact tacaggcggg cgtaaaatgg ccagaacgat
    55261 gacggttgat ctcggcgatg agttgcgtga atttatcgag tccttaattg aatccggcga
    55321 ctatcgcacc caaagcgaag tgatccgtga gtcactgcga ttacttcgag aaaagcaagc
    55381 agagtctcgc ctgcaagcgt tacgtgacat gctcgcagaa gggttgagta gtggtgaagc
    55441 tcagccgtgg gaaaaggacg cgttcttacg gaaggtgaaa gcagggataa ggaaataatg
    55501 tgcgtattat taaactcatg ccaaaagcca atgaggattt agaaggcatc tggtattaca
    55561 gctatcacca tttcggcgaa ccacaagccg acagatatgt ggaacatctt tcagacgttc
    55621 tccagattct gagtaacaat aatataggta cgcccagacc tgaattaggc gaaggtattt
    55681 ttgttcttcc ttttgaacgc cacgtcattt atttcctgca atcaccaggg gaaattatcg
    55741 tcattcgtat ccttaaccaa aaccaggacg ccaccagaca tttacactgg agctaaaccc
    55801 gccgattgcg ctacaatgcc cgcccttaaa gtgggggcac tcccctaacc gcttcatcag
    55861 gtgaagcgga tctgacctgt catcagaacg agagaattat gtttaaaccg gaactccttt
    55921 ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc ttatggcgca gatgctgttt
    55981 atgcgggcca gccgcgttat tccctgcgtg tgcgcaacaa cgaattcaac cacgaaaatc
    56041 ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca
    56101 ttgcaccgca caacgccaag ctgaaaacct ttatccgtga cctgaaaccg gtggtggaaa
    56161 tggggccgga tgcgctgatt atgtccgatc cagggctgat tatgctggtg cgtgagcact
    56221 tccctgaaat gccgatccac ctttcggtgc aggctaacgc cgtgaactgg gcgacggtga
    56281 aattctggca gcaaatgggc ctgacccgcg tgatcctctc tcgcgagctg tcgctggaag
    56341 agattgaaga gatccgcaat caggtgccgg atatggagat cgagatcttc gttcacggcg
    56401 cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg ctatatcaac aagcgcgacc
    56461 cgaaccaggg cacctgcacc aacgcctgcc gctgggagta caacgtccag gaagggaaag
    56521 aagatgatgt tggcaacatc gtacacaagt acgagccgat tccggtgcaa aatgttgagc
    56581 cgacgctggg tatcggcgca ccaaccgaca aagtgtttat gatcgaagag gcccagcgtc
    56641 cgggcgagta tatgaccgcg tttgaagatg agcacggcac ttacatcatg aactcgaaag
    56701 atctgcgcgc catcgcccat gtagaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa
    56761 tcgaaggtcg taccaaatct ttctactatt gtgcacgcac cgcacaggtt taccgcaaag
    56821 ctatcgatga cgccgctgcg ggaaaaccgt tcgataccag cctgctggaa actctggaag
    56881 gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg tcatactcac gacgattatc
    56941 agaactacga atacggttat tcagtttctg accgccagca gtttgttggt gagtttaccg
    57001 gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa taaattctcc gttggcgaca
    57061 gccttgagct gatgacgccg caaggcaaca ttaattttac ccttgagcac atggaaaacg
    57121 ccaaaggcga agctatgccg atagcaccag gcgatggtta tactgtgtgg ctcccggtcc
    57181 cgcaggatct tgagctcaat tacgcgctgc tgatgcgtaa tttctccggg gaaaccacgc
    57241 gtaatcccca cggtaagtga ttaatttcga ttatttttcc cggatggaaa attcttagaa
    57301 accgatcaca tacagctgca tttattaagg ttatcatccg tttcgctgaa aaacataacc
    57361 cataaaatgc tagctgtacc aggaaccacc tccttagcct gtgtaatctc ccttacacgg
    57421 gcttattttt tatgcataag ccctatccct ggtcaccgtc ttccattgac cacatcgata
    57481 gaatctccct tcatagcacg atgcctttca cgtaacggca tcgtgctcgc acaggttccg
    57541 gctaagcaca accagaacgc gcatgtttga cgcttaccaa aaaatattct cactctccac
    57601 atttgaatgt cagacgagcg acgccatgta atcctgcacc ttctgtcttc aggtcaacta
    57661 tctgcatttt tttgccctga gtaacacaga aatgggctgc atcatttttt actatatttt
    57721 ctgcaccaga tattctgccc ctggctaaag aagcttcggc ttcggtgtag tattggttat
    57781 cgagtttacg ctgaatatta cttttatatg caagaccaaa tttaccgata cttgtctcat
    57841 cattatgcac agcacaacca gacataataa aaatactaat taatgatata gcagctatct
    57901 ttttcatctc accttccccc attaaatacc aacgacactc tctagtgttt aaatataata
    57961 atggcatgat tattataatt gaataggatt ataataaatg ttctgtacaa catttcctac
    58021 ataagtagga attacggaca ttgaggccct tcagggtaac tccatggggg ctttaatata
    58081 tttggaatag tatatttaag atgccactgt ttggttgaat attaggcata tgctcttttt
    58141 tgaaatttat cggtggcagc cgttagtatt cgctgtcccc attgcaagct cctggtggta
    58201 accactgaat cctccatact tgaactgact ttttatcctc cgactttcat cctgttctga
    58261 ctccaccttt tgttttctgc tctacactat ctacagacca atcataaagg cacatacgat
    58321 catggcagaa tttcccgcca gcttactgat tcttaatggc aaaagtactg acaatctacc
    58381 cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac
    58441 ctgggagaaa ggcgatgccg cacgatatgt agaggaggcc cggaagttgg gcgtcgcaac
    58501 ggtgattgcc ggtggtggcg atggcaccat taatgaagtt tctacggcgt tgattcagtg
    58561 tgagggggat gacatacccg cgctgggaat tttgccatta ggaaccgcca atgattttgc
    58621 caccagtgta gggattcctg aggcactgga taaggcgctg aaactggcaa ttgccggtaa
    58681 cgccattgcg atagatatgg cgcaggtcaa caaacaaacc tgttttatta atatggcgac
    58741 aggcggattt gggacgcgta ttaccacaga aacgccggaa aaattaaaag ccgcgctggg
    58801 tggcgtctct tacatcattc atggcttaat gcgcatggat actctgcaac cggaccgttg
    58861 tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc ctggtcattg gtattggtaa
    58921 cgggcgtcag gccggtggcg gtcagcaatt gtgtccgaac gcgttaatta acgatggctt
    58981 gctgcaactg cgcattttta ccggcgatga aattcttccg gctctcgtat caaccttaaa
    59041 atctgacgaa gataacccga atattatcga aggcgcttcg tcgtggtttg atatacaagc
    59101 cccacacgaa atcactttta atcttgatgg cgaaccgttg agcggacaaa actttcatat
    59161 tgaaatactt ccggcggcgt tgcgttgtcg attaccacca gattgtccat tattgcgtta
    59221 atcaaactat tctctcttga accagggaga atccgaaatg ccggatgcgg tgtaaatacc
    59281 ttatccggca tacagaacaa tactacaccg taattaattt tacccgtgtc ttctgcaatg
    59341 ccagtttatc cacctctgat acagcatcat cggtaataat aatgtcaatt ttttcgattg
    59401 gcagtacctg attaaaaccg cgacggttaa atttcgatga atcgagaacg gcaatcactt
    59461 tattagcggc ggtgaccatc gcaccgctaa tcgaataacc ttcattaaag gtcgtaatac
    59521 cattaaccgc atcaatacca tcagcaccga caaacattaa atcggcatta atatcctgca
    59581 gggaacgttc agcaatagat ccatgcatag atcgcgtttt atggcggacc gtgccaccgc
    59641 agacgaccag agtaatgtct ttattttcgg aaagggcaaa cgcagccggg agactgttgg
    59701 taatcaccgt gatatttttg gcggtcatta atccttcggc gataagcatt gtggtacttc
    59761 cgctgtcgag aataacggtc atcccttcat ggatcatttt gactgccgcc tgcgctattc
    59821 tgtttttcgg cgcgctggcg agctgaaatc gctctttaaa cccgacttcc tgggtctcgg
    59881 tttcactatt accggacatt attttcgccg caccgccatg aaagcgcgta accacgcctt
    59941 tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc cgaggcagca aatactcccg
    60001 ccagatcctg aacaagcacg gttccctgtt cattcactaa ttggatgatc ttattccttc
    60061 gctcgaatga attcatcgtc atttacccat attcaattgt ggctagtgta aacgaagggg
    60121 gaccattggt gaatgatctg ttttgcacaa taccgggcca gcatcacgct ggcccgcggt
    60181 ttcagggaat gagcaacact ttgcccggca tagcattacg agcgatgtca cgcaccgcct
    60241 gggcgaagct ttcaaagctt ccacggtgag cgattaatgg ctccaggctt aacttacgtt
    60301 ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg ccaagggctg gagtagttca
    60361 tccaactgcc gataaccgtc agctctttac gcaatatttt gccaaacgtt gccgatgtta
    60421 aatgcagatc ctgatgcaac gtgcccacca gcgccagttg ggcatgagga ccggcaatct
    60481 ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc gaggataagc tgattaaagc
    60541 gcagttagcg taaaacgctc tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt
    60601 gcatcgcacc gaaagatttt gccagtgcca gtttttctga actaatgtcg atcgccgtca
    60661 cactctttgc tcccagcgcg acagcgcact gaatggccag caggccaatg gttccggcac
    60721 caataataat aacgttttta ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa
    60781 cggtaatcgg ttcaataaaa gccccatcct caataggcat atcagtaggt agagcaaaga
    60841 catttttccg cttaacgaca atatattcgg caaatccacc atcacgccgt gagccaataa
    60901 aatcatattt tgcgcactgg gaataaaacc ctttcaaaca ctctggacaa gtaaaacagg
    60961 gtaataacgg cacacaggca accgcatcgc caggatgtaa atcatcaaca ccggatccca
    61021 ccgcatcaat atagccgcta aattcatggc ctaacgttat tggataataa tgtgcaccat
    61081 ttttaaatat cctgggtaaa tcggaaccac ataagcccga gctggcaatt tttacccgca
    61141 cctcatcctg atgtttaatt tcaggaatga cgctttctgc aacgcgcacg ataccatcag
    61201 tatcattcac cactgatttc ataaaaactc ctgattgtta agggggataa cctccccctc
    61261 aggtaaaaat tattctgcga gaacgacttt ctcttgttta ataaagccac gcgctctacg
    61321 ccaggtcatg aaaataccgg taagataaat tgcgccgata ataatgaatc cgggaatatt
    61381 ttgcggggag aaaacctgaa tcagtaacca ggtaatggga gaaccgccct gatccattga
    61441 agccaccata cccccggctt ttaacgcccc agcattagcc gccagttggg tgtgcaaacc
    61501 aatagtttgc gtcgcgatcc acagagtgat gctcatgatg atgacacccg agattaaggt
    61561 gcggaacaga tttccacgat gcacggcgac tgccatcgcc acaaagaagc cgatggtggc
    61621 aagatcgcca aacggcagca cctgattacc cggaacacaa acagcaatta aaatggtgag
    61681 cgggataaaa atcaggcttg ccgataccac cgccgtatgc cccagcagta atgcgggatc
    61741 caggccaatc aggaactcct gaccgccgaa cttcgcctgt aaacgactac gggcctgctt
    61801 agcgatgggc gttaaaccat ccatgatggg tttaatcacc cgtggcatta gcagcatcac
    61861 tgccgccgtt tttaccgcca gctgcaatac acctttgaca tcgtaacccg cgaggatgcc
    61921 gataatcagc cccatcacaa aacccacggt gacaggctcg ccaaatggac caaatttgcg
    61981 ctgaatatcg tcggcgctaa atttaattcg gttaacgcct gggattttct cgatgatagc
    62041 atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa tagcaatacc
    62101 ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct tataaacaaa
    62161 cgccgcgtga attaccacac ctgccatccc tatcatccat gaaccggttg ccaggtgcag
    62221 caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta ccacccgcgt
    62281 catacgggtc agtaacatcg ccacgttaac caatatcgca atcggaatcg ccaccagcgc
    62341 aatttgcgac gcccaggtca ttggtgaaga gcccggccag ccgacatcga ccacatgcag
    62401 attcaggtcg aaattttccg ccatcgcttt cgccgccgga ccaatggaat ccagcattaa
    62461 gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacccg ctttaaagca
    62521 atcgcctgcc ttcatgccta atattttaga aaaaataatg atgacaatcg gaagcatcac
    62581 cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc acccctgtaa
    62641 gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaataa aaggcatgcc
    62701 gtgaactaac ggaatatcgc caaaactacg atccactttg gcagtggtgc atatcaaatg
    62761 cacaccatcc atataggttt ctatttcatt aacccgacac tggattaatt caacaggaat
    62821 attatgattc tgacacaact ctttaatttc ttccgccgcc atcgtagagg tcgcaaccgc
    62881 gcctccgcaa gcgacaataa tcttgcgttt cataaatagt cctttttatt gttacagagg
    62941 gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact gagtttcagg
    63001 aagagtaatt agtgtctcga cgatatcggg ctgttgtaac ttgccaaata aacagcgtaa
    63061 aagtttcaat tgctgctgcg gattttccac aatcaacgca ataaccaacg acacctccac
    63121 gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta acagataaat
    63181 ggctgacgac ttagcatgaa tcgcctcaca atgcggtata gcaatggcgt gctgctcaag
    63241 cattatcccg gtagggaatt ctgcttctct ggcaattaat gcctgtggcc aggtatcata
    63301 aaccacacct ttcgcgagca tctcattacc gatatgggtt aaaacttcgc tacgatcgac
    63361 aaaagaaatt ccgctacgaa caaacaggtt agtcatatac cgtccttatt ccgcacagcc
    63421 gtagcgatag gcgcgcaaaa catcataaat tttatccata atcagctgat gcggtattgc
    63481 tgataattcc ccggactgaa tgcgttcaaa ttgtttggga agatactgac taatcatgcc
    63541 cagtgggatg tccacgcctt caagattcac catcatcgtt tcgacgctat ttttaatccg
    63601 actatgcggc cagtaataac gaatacgatc cgacaggctg taacgaatat ccagtaatga
    63661 atcgttaaaa cccgtacgat aatatttttt ccagtattgc ggttcgtcga gcatcacttc
    63721 ttcaattacc gccaggcaac cgctgcgatt ttcaggggcg ataagttcct gctcaatttg
    63781 tgccagtgca aatatcgcct cgcgtaaagc aaaggttaat gcgggaccga ctttcaatat
    63841 tgcaaagtga tcgcggacta attcccaata agccgtccgg gtctggtaat cggtagaatg
    63901 tgcttcataa accattcggg tgctttctat ccattgcgcc agcggctgcg cttcctgcgg
    63961 ctgataatgg ataatattgc tgtgatcaaa ttccacgccc ggctgcacca cgatggcaat
    64021 cacgcgtgtt aacgcctctg tcagcccacg ggcaataaag gccttttgat gcgtacgtaa
    64081 agtattggcg gcatcttcaa catgggtgat gtgtactgac tgaatggcgc tggcctcacc
    64141 gcccggaacc ggaacttcgg tgccaatgac atagctcagt tgctcacgct ggcaatctgt
    64201 cgccacactt tccgcagcaa agcaaagcac agcagctcgt tccgcaaccg tttctggtgc
    64261 taacggtatg ggatcccccg cgcaggacat tgacgcatca agatgaattt tactgaaacc
    64321 ggcacgaaca tattccttta ccagctcgac ggatttttcc atcgccgcat ccgcattttc
    64381 ttgctgccag cagtttggcc ccagatgatc gccgccgaga ataatgcgtt cgcgtgcaaa
    64441 cccaacttta tcggcaatcg taaaaacaaa ttcgcgaaag tctgccggtg tcattccggt
    64501 ataaccgcca aattgattga cctggtttga cgttgcttca atcagcactt tgcgcgtgct
    64561 gttgcgatca aatgccagcg ccgcttcgat aaccaacgga tgggcagaac agactgaaca
    64621 tatgccgata tgttcaccag ctttatgccg ggcaattaac gttttcatgt tttttcctta
    64681 ttaaatggca ggtgcgttat gccctgccct cgcagccaca atcggcaatc actttgctca
    64741 ccacatcgcg cattgcggat ttagccgact gcaaataatc ccggggatcg gtcgcttcag
    64801 ggtgctcggt caggtaattt tttaacgcct gcgagaaggc atttttcagc tccgttgcaa
    64861 cgttgatttt gcatatcccc agtttgatgg tttgctgaat atccttagtc gataaccctg
    64921 acgcgccatg cagcaccagc ggtaagttca cccactggcg aatgttctcc agtcgagaaa
    64981 aatcaagcgc cggtgcgctg gcatacatcc catgagccgt gccgatggcg accgccaggg
    65041 aatcaattcc ggttgcctcg gcaaattcac gcgcctgagc ggggttggta taaaaagcat
    65101 cggcttcatt gacttgcaca tcatcttcct ggccgccaag ttgccccagc tccgcttcga
    65161 cgctgacatc aaagcgatgg caaaaatcca ccacctcttt gacccgtgaa atattttgcg
    65221 caaaaggcaa atgcgaggcg tcaatcatga ctgagcgcac gccagaacga accttctgag
    65281 cgatatcgtc aaatttcgtg tgatggtcga gatgaattgc cagtggatgg tgatattgct
    65341 tcgccatcgc gctgaccagc gccaacagat tttctgtacc agcatgagta aatgtgccag
    65401 gcgttccggc gatgatgacc ggcgcatgca ggttggcagc ggtttccacc accacttgca
    65461 tcgtttcgag attgtgaata ttgaatgccg gaaccgcata accgccgcgc tgtgcgttgt
    65521 tcagcatctg ctttgtcgat accacgtaca ttttcat
//
LOCUS       DAZKGS010000007        62903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKGS010000007 DAZKGS010000000
VERSION     DAZKGS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHOQY_000463"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHOQY_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9676623.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHOQY_000464"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHOQY_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9676624.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHOQY_000465"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHOQY_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9676625.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHOQY_000466"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHOQY_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9676626.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHOQY_000467"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHOQY_000467"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9676627.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHOQY_000468"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHOQY_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9676628.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHOQY_000469"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHOQY_000469"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9676629.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHOQY_000470"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHOQY_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9676630.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHOQY_000471"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHOQY_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9676631.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHOQY_000472"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHOQY_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9676632.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHOQY_000473"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHOQY_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9676633.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="ACHOQY_000474"
     tRNA            complement(13705..13781)
                     /locus_tag="ACHOQY_000474"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="ACHOQY_000475"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="ACHOQY_000475"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9676634.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHOQY_000476"
     CDS             complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHOQY_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9676635.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17564..17650)
                     /locus_tag="ACHOQY_000477"
     tRNA            complement(17564..17650)
                     /locus_tag="ACHOQY_000477"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHOQY_000478"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHOQY_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9676636.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHOQY_000479"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHOQY_000479"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9676637.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHOQY_000480"
     CDS             complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHOQY_000480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9676638.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHOQY_000481"
     CDS             complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHOQY_000481"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9676639.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHOQY_000482"
     CDS             complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHOQY_000482"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9676640.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHOQY_000483"
     CDS             23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHOQY_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9676641.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHOQY_000484"
     CDS             complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHOQY_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9676642.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24455..25888
                     /gene="dacB"
                     /locus_tag="ACHOQY_000485"
     CDS             24455..25888
                     /gene="dacB"
                     /locus_tag="ACHOQY_000485"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9676643.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHOQY_000486"
     CDS             complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHOQY_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9676644.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHOQY_000487"
     CDS             complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHOQY_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9676645.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHOQY_000488"
     CDS             complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHOQY_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9676646.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHOQY_000489"
     CDS             complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHOQY_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9676647.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29056..30027
                     /gene="ispB"
                     /locus_tag="ACHOQY_000490"
     CDS             29056..30027
                     /gene="ispB"
                     /locus_tag="ACHOQY_000490"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9676648.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHOQY_000491"
     CDS             30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHOQY_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9676649.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHOQY_000492"
     CDS             complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHOQY_000492"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9676650.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHOQY_000493"
     CDS             complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHOQY_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9676651.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHOQY_000494"
     CDS             complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHOQY_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9676652.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHOQY_000495"
     CDS             complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHOQY_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9676653.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHOQY_000496"
     CDS             complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHOQY_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9676654.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHOQY_000497"
     CDS             complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHOQY_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9676655.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHOQY_000498"
     CDS             complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHOQY_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9676656.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHOQY_000499"
     CDS             35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHOQY_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9676657.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHOQY_000500"
     CDS             36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHOQY_000500"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9676658.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHOQY_000501"
     CDS             37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHOQY_000501"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9676659.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38181..38756
                     /gene="lptC"
                     /locus_tag="ACHOQY_000502"
     CDS             38181..38756
                     /gene="lptC"
                     /locus_tag="ACHOQY_000502"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9676660.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38725..39282
                     /gene="lptA"
                     /locus_tag="ACHOQY_000503"
     CDS             38725..39282
                     /gene="lptA"
                     /locus_tag="ACHOQY_000503"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9676661.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39289..40014
                     /gene="lptB"
                     /locus_tag="ACHOQY_000504"
     CDS             39289..40014
                     /gene="lptB"
                     /locus_tag="ACHOQY_000504"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9676662.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHOQY_000505"
     CDS             40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHOQY_000505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9676663.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41518..41805
                     /gene="hpf"
                     /locus_tag="ACHOQY_000506"
     CDS             41518..41805
                     /gene="hpf"
                     /locus_tag="ACHOQY_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9676664.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHOQY_000507"
     CDS             41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHOQY_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9676665.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHOQY_000508"
     CDS             42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHOQY_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9676666.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43311..43583
                     /gene="npr"
                     /locus_tag="ACHOQY_000509"
     CDS             43311..43583
                     /gene="npr"
                     /locus_tag="ACHOQY_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9676667.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHOQY_000510"
     CDS             43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHOQY_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9676668.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHOQY_000511"
     CDS             complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHOQY_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9676669.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHOQY_000512"
     CDS             complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHOQY_000512"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9676670.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHOQY_000513"
     CDS             complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHOQY_000513"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9676671.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHOQY_000514"
     CDS             complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHOQY_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9676672.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50070..54530
                     /gene="gltB"
                     /locus_tag="ACHOQY_000515"
     CDS             50070..54530
                     /gene="gltB"
                     /locus_tag="ACHOQY_000515"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9676673.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54543..55961
                     /gene="gltD"
                     /locus_tag="ACHOQY_000516"
     CDS             54543..55961
                     /gene="gltD"
                     /locus_tag="ACHOQY_000516"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9676674.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHOQY_000517"
                     /pseudo
     CDS             56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHOQY_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHOQY_000518"
     CDS             complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHOQY_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9676675.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHOQY_000519"
     CDS             complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHOQY_000519"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9676676.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHOQY_000520"
     CDS             complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHOQY_000520"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9676677.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHOQY_000521"
     CDS             complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHOQY_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9676678.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHOQY_000522"
     CDS             complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHOQY_000522"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9676679.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHOQY_000523"
     CDS             complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHOQY_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9676680.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15395 a        17093 c        15527 g        14888 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg cccagcaact
     1681 gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt acttcacccg
     1741 gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg ttaccgatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatcctgct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgcagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtatagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tgtccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc
     5161 ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg atcggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagag gaaccgttgg attcagtgat ttcagacaca acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcagta cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc gatttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tggtttcgcc gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacgaccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    13801 tactcgtttt gggtaaaaga tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaataac
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aggcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cgcccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgagat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    20281 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    20341 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    20401 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    20461 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    20521 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    20581 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    20641 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    20701 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    20761 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    20821 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    20881 agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc cagattggtc
    20941 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccata gatgatctct
    21001 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    21061 ctgattgcgt cgccttcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    21121 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    21181 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    21241 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    21301 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    21361 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggggccaa tggtacgcga
    21421 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    21481 tgacggtcga aacggccagg acgcagcagg gccgggtcga gaacgtccgg acggttagtc
    21541 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    21601 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    21661 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    21721 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    21781 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    21841 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    21901 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    21961 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    22021 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    22081 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    22141 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    22201 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    22261 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    22321 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    22381 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    22441 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    22501 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    22561 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    22621 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    22681 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    22741 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    22801 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    22861 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    22921 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    22981 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    23041 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    23101 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    23161 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    23221 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    23281 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    23341 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    23401 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    23461 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    23521 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    23581 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    23641 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    23701 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    23761 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    23821 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    23881 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    23941 accgaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    24001 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
    24061 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    24121 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    24181 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    24241 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    24301 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    24361 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    24421 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    24481 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    24541 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    24601 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    24661 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    24721 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    24781 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    24841 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    24901 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
    24961 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
    25021 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    25081 aggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
    25141 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    25201 ggatggagcc agctatgccg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    25261 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    25321 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    25381 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
    25441 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    25501 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    25561 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    25621 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    25681 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    25741 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
    25801 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    25861 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
    25921 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcat
    25981 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
    26041 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    26101 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
    26161 agatcagata atatttatct tcccagccca gcgcttcagc aatcgctttc gctttctctt
    26221 cagcttctgc cttatccagc agatcgatct tattgaacac taaccaacgc ggtttcgccg
    26281 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    26341 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    26401 gcttcaggaa gcggataccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    26461 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    26521 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    26581 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    26641 gcagcagctc acgcttatca cccggcgtac cgttggtttt ctgccgtggt gtacggttaa
    26701 cggacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    26761 gctgaccgtg tttagtcata tcgcccatgg tttcaccagt accttggtcg attatacgcg
    26821 tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacaa tcgcggcttg
    26881 cgccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
    26941 tcaggttttc gtcggcttcc atccatacgt caccaccatc accaccgtcg ccgccatccg
    27001 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    27061 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    27121 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    27181 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    27241 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    27301 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    27361 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    27421 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    27481 caattgtaca taaagcgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    27541 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    27601 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    27661 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    27721 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    27781 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    27841 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    27901 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    27961 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    28021 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    28081 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    28141 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    28201 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    28261 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    28321 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    28381 cgccaccgaa acgcttaacg cccaagcgtt tagcttctga atcgcgaccg ttacgtgtgg
    28441 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    28501 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    28561 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    28621 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    28681 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    28741 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    28801 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    28861 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    28921 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcactc
    28981 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    29041 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    29101 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccagctgat caatcagtta
    29161 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    29221 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    29281 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    29341 aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    29401 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    29461 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    29521 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    29581 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggaaga ggagaaaggc
    29641 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    29701 tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg acgatctgaa cgaaggtaaa
    29761 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    29821 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    29881 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    29941 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    30001 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcggggt
    30061 ggttccagta aattccataa aaacacttac tcagctctat aacacctgaa aaacaggctg
    30121 tgcatcttta tatattctga atattcacac tccttacagg aactttttag agcaatcggc
    30181 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    30241 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    30301 tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt tgagttcctc
    30361 aacgctggcg aatgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    30421 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    30481 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    30541 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    30601 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    30661 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    30721 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    30781 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    30841 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    30901 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    30961 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    31021 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    31081 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    31141 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    31201 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    31261 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    31321 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    31381 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    31441 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    31501 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    31561 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    31621 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    31681 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    31741 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    31801 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    31861 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    31921 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    31981 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    32041 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    32101 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    32161 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    32221 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    32281 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    32341 caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt tgttgccctg
    32401 ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat ccacgcggga
    32461 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    32521 cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    32581 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    32641 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    32701 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttt
    32761 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg gccattcggg
    32821 tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg ttctggcgca
    32881 atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    32941 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    33001 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    33061 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    33121 aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc tgccgcggtt
    33181 gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    33241 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    33301 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    33361 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    33421 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    33481 tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    33541 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    33601 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    33661 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    33721 gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa gatacccacc
    33781 caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg cggtcagcac
    33841 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    33901 aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc acgtcaccgt
    33961 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    34021 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    34081 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    34141 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    34201 gctggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    34261 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    34321 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    34381 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    34441 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    34501 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    34561 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    34621 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    34681 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    34741 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    34801 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    34861 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    34921 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt
    34981 cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc atcatcactg
    35041 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    35101 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    35161 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    35221 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    35281 ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    35341 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    35401 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    35461 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    35521 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    35581 tcagcggacc ggcgagtata cctgaagaaa ggatgttaga tgcttttagc tacggcactg
    35641 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    35701 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    35761 ggtacatcgt taccagaagt catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    35821 ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat cctcggtctg
    35881 gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga attaccctta
    35941 atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact tagtcgcagc
    36001 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    36061 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    36121 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    36181 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    36241 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    36301 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    36361 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc aggagagatt
    36421 gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    36481 ttgctgtgct ggcggcgctc cccacaaccg ggccgtggtg taggggtatt attaactggc
    36541 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    36601 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    36661 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    36721 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    36781 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    36841 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    36901 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    36961 tgtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    37021 ggcgcgcgct gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    37081 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    37141 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    37201 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    37261 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    37321 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    37381 tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat
    37441 gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    37501 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    37561 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    37621 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatggca
    37681 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    37741 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    37801 attcgttgtg tgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    37861 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    37921 aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga aaatgtggct
    37981 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    38041 gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg cattgctggc
    38101 ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg caaactggat
    38161 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt gtgctatcac
    38221 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    38281 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    38341 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    38401 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    38461 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    38521 acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg acggataacg
    38581 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    38641 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    38701 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    38761 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    38821 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    38881 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    38941 gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    39001 gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc ttcccagatg
    39061 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt
    39121 gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa aatgcaggct
    39181 ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa
    39241 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    39301 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    39361 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    39421 actttctata tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    39481 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    39541 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    39601 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    39661 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    39721 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct cgacgaaccg
    39781 tttgccggtg ttgacccgat ctcggttatc gacattaaac gcatcattga gcacctgcgc
    39841 gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    39901 gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc
    39961 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    40021 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    40081 tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt cgtctgttgc
    40141 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    40201 ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac agtgaaacgc
    40261 tggacaccgc cgatgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    40321 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    40381 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    40441 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    40501 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    40561 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    40621 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    40681 ataaaaccac gccgtggctg gaagaggcca gattgatcat tagcgatcat ctcgatctgt
    40741 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    40801 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    40861 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacag
    40921 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    40981 gcaataacgc tcgcaatgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    41041 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    41101 tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa ccgatggtac
    41161 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    41221 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    41281 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    41341 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    41401 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    41461 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    41521 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaca
    41581 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    41641 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    41701 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    41761 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    41821 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    41881 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    41941 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag
    42001 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    42061 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    42121 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    42181 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    42241 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    42301 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    42361 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    42421 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    42481 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    42541 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct ggccgatcgc
    42601 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    42661 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt cctggatgcc
    42721 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    42781 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    42841 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    42901 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    42961 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    43021 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    43081 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgaa
    43141 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    43201 accggcggaa agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    43261 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    43321 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    43381 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    43441 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    43501 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    43561 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    43621 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    43681 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagagacatt gtttaggttt
    43741 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    43801 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    43861 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    43921 agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg
    43981 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    44041 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    44101 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    44161 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca
    44221 gtgagtcaga ggttatcccg gtcgtctgtg acaacattgg tgaaagcaca ttgattccac
    44281 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    44341 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    44401 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    44461 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    44521 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    44581 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa
    44641 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    44701 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    44761 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    44821 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    44881 agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga
    44941 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    45001 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    45061 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    45121 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    45181 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    45241 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    45301 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    45361 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    45421 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    45481 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    45541 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    45601 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    45661 aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    45721 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    45781 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    45841 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    45901 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    45961 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    46021 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    46081 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    46141 gaaggtccgg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    46201 ccgccgcacc tttaatttta tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca
    46261 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    46321 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    46381 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    46441 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    46501 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    46561 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    46621 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    46681 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    46741 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    46801 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    46861 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    46921 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    46981 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    47041 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    47101 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    47161 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    47221 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    47281 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    47341 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    47401 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atacggctca
    47461 ggccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    47521 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    47581 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    47641 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    47701 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    47761 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    47821 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    47881 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    47941 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    48001 tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    48061 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    48121 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    48181 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    48241 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    48301 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    48361 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    48421 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    48481 tatccacgga cgttccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    48541 ctcgaccatt cccgtccatc gattttcgca ccacagagga gcaagcagcg tcgggcgacg
    48601 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    48661 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    48721 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    48781 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac
    48841 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    48901 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    48961 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    49021 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    49081 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc
    49141 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    49201 ctcggcaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    49261 gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt
    49321 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    49381 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    49441 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    49501 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    49561 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgtcatta
    49621 ccaattaagg cagtataaaa tgctggcttt ctcgtcagtt caaggcagga taagggttaa
    49681 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    49741 agataaaatc tattttaatt tcagtcattt aataaagaat tttgcgctaa agcacatttc
    49801 tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct
    49861 ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct
    49921 tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga
    49981 cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg gaagtggggt
    50041 tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata
    50101 actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta
    50161 ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa
    50221 ccggcgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc
    50281 aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg gatgctattc ctgaataaag
    50341 atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct
    50401 tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc
    50461 tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc
    50521 gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg
    50581 acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa ggtctgtgta
    50641 tgccggcgga tctgccgcgc ttttatctgg atcttgcgga cctgcgtctg gaatcggcca
    50701 tctgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg ctggcgcagc
    50761 cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac cgccaatggg
    50821 cgcgtgcgcg tacctataaa ttccagacgc cgcttatccc tgatctgcac gacgccgcac
    50881 cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactgctgc
    50941 tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc gcctggcaga
    51001 acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg
    51061 agccgtggga tggcccggcg ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc
    51121 tcgaccgtaa cggtctgcgt ccggcacgct acgtcattac caaagacaag ctcatcacct
    51181 gcgcctctga agtcggtatc tgggattatc agcctgacga agtggtcgaa aaaggccgcg
    51241 tcgggccagg cgaactgatg gtgatcgaca cccgcagtgg gcgtattctg cactcggcag
    51301 aaaccgatga cgatctgaaa agccgccatc catataaaga gtggatggag aaaaacgtcc
    51361 gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaactggacg
    51421 acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag ctggactccg
    51481 taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc gatgataccc
    51541 cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc cagcagtttg
    51601 cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca
    51661 ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg caccgtttaa
    51721 gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg atgaaagagg
    51781 agcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag
    51841 cgacagtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc accgtgctgc
    51901 tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg
    51961 ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg
    52021 tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggc ttcggcgcga
    52081 cggctattta tccatacctt gcctatgaaa cgctgggccg cctggtagac acccatgcga
    52141 ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac aaaggcttgt
    52201 acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt
    52261 ttgaagcggt cggtctgcac gatgatgtag tgggcctgtg cttccagggg gcggtcagcc
    52321 gcattggtgg ggcgagcttt gaagacttcc agcaggatct gctgaacctg tcgaaacgtg
    52381 cctggctggc gcgtaagccc atcagccagg gcggcctgct gaaatacgtc cacggcggcg
    52441 aataccacgc ttacaacccg gacgtggtgc gcacgctgca acaggcggta caaagcggcg
    52501 agtacagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctgc
    52561 gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg
    52621 caagcgaact gtttaaacgc tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg
    52681 aagcccacga ggcgctggcg gaagcgatga atagcatcgg cggtaactcg aactccggtg
    52741 aaggcggcga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg
    52801 cttctggtcg cttcggcgtt actccggcgt atctggtcaa tgccgacgtc attcagatta
    52861 aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc
    52921 cttacatcgc caaactgcgc tattcggtgc ccggagtgac gctgatctcc ccgccgccgc
    52981 accacgatat ctactctatc gaggacttag cgcagctcat tttcgacctc aagcaggtta
    53041 acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga
    53101 ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggtggcaccg
    53161 gcgcaagccc gctgtcgtca gtgaaatacg caggttgccc gtgggagctg gggcttgttg
    53221 aaacccagca ggcgctggtt gccaatggtc tgcgtcataa gatccgtttg caggtcgatg
    53281 gcggcctgaa aaccggtgtt gatatcatca aagcggcgat tctcggcgca gaaagcttcg
    53341 gcttcggcac tggcccgatg gtggcgctcg gctgtaaata tctacgtatt tgccatctga
    53401 acaactgcgc aacgggtgta gcaactcagg atgacaaact gcgtaagaac cactatcacg
    53461 gcctgccgtt caaggttacc aattactttg aatttattgc ccgtgaaacc cgcgagctga
    53521 tggcacagct gggcgtaacg cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag
    53581 aactggacgg tttcaccgcc aaacagcaaa aactggcgct gtcgaagctg ctggagaccg
    53641 ccgaaccgca tccaggtaag gcactctact gcaccgaaaa caacccaccg tttgataacg
    53701 gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca
    53761 aaaccttctg gttcgatatt cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct
    53821 atatcgccca gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca
    53881 acggcaccgc aggccagagt ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga
    53941 ccggggatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc gccattcgtc
    54001 ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac acctgcctgt
    54061 atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc
    54121 ggaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga
    54181 cgggcggtat tgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg
    54241 gcggcttcgc ttacgttctc gatgaaagcg gtgatttccg caaacgcgtt aacccggaac
    54301 tggtcgaggt cttaagcgtt gacgatctgg cgatccatga agagcatctg cgcggtctta
    54361 tcaccgagca tgttcagcat actggctctc agcgcggtga agagattctg gcgaactggt
    54421 caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc
    54481 tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa
    54541 caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg ccaaagaaac
    54601 cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc gaaggccagg
    54661 ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc
    54721 cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt atttttgaag
    54781 cggcggaact gtcgcaccag accaaccccc tgccggaagt ttgcggacga gtctgcccgc
    54841 aagaccgtct gtgcgaaggt tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg
    54901 gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt ccggatatgt
    54961 ccggcgtgaa gcaaaccggt aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg
    55021 cgtgtgcgga tgtcctgacg cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag
    55081 aaattggcgg gctgctgacc ttcggtattc cggccttcaa gctggaaaaa gaggtaatga
    55141 cgcgtcgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg
    55201 gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtcttc cttggcgtcg
    55261 ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag
    55321 cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg
    55381 aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga
    55441 tggactgcgt gcgtacgtcc gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc
    55501 gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg
    55561 tagagttcaa attcaacgtc cagccactgg ggattgaagt gaacggtaac ggtaaagtca
    55621 gcggcgtaaa aatggtacgt accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg
    55681 cggagatcgt tgcaggttcc gaacatatcg ttccggcaga tgcagtgatc atggcgtttg
    55741 gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcacaag
    55801 gccgcatcat cgccccggaa ggcagcgaca acgccttcca gaccagcaac ccgaaaatct
    55861 ttgctggcgg cgatatcgtc cgtggttccg atctggtggt aaccgctatt gccgaaggtc
    55921 gtaaggcggc agacggtatt atgaactggc tggaagttta agctctgtcc cagcgccgcc
    55981 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    56041 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    56101 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    56161 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcatctg gtcgatagcg
    56221 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    56281 gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc
    56341 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    56401 acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt cgccagacag
    56461 tgtctggcga attaacacct gccctggtcc acctacgtcc gtttactttc cgttaaaaac
    56521 gctgacgccc gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag
    56581 ctagaacggc aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca
    56641 gccatgctgc aacaacacgc tcctaccgag acgcatattc ttccgcaaca ggcgatacgc
    56701 gatccctttg tgctcgaatt tctggaattg aaagatgaat attcagaatc tgatttcgag
    56761 gaggcgctga tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt
    56821 gttggtcggc agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt
    56881 ttccaccgcc gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat
    56941 agcgatgccg gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg
    57001 gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat
    57061 tatgctctgg caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct
    57121 gatgagaaac gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat
    57181 agacgccgtt aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa
    57241 cgaactaagt tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct
    57301 ccaggctcgc cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac
    57361 attcccggtt ttaagatgat ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca
    57421 tcctcatggt ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt
    57481 tcctcaccgt ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc
    57541 tcgacgggcg attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat
    57601 tcgtaacgac ccggcgcttt ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc
    57661 tgtaacgcag gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt
    57721 tttctccctg ggccaacagc gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg
    57781 ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca
    57841 gatacccttc tgccagccca acgctgccac cgaccaccac gcactggcaa tcagttgtgg
    57901 ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcggtgaatc agctgctgcg
    57961 cctgctcgtc gccctgcccg gcgcgtgtga aaatagttct cgcatccgcg cccgccagtt
    58021 caccctgcgc tgccgccgca atgccgcgac cagaagcaat cgcctccacg caacccgtgc
    58081 gtccacagcc acagaccggg ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca
    58141 gaccgccagg gccggtaagc agtttgccgc cgctcactac accgccgccg acgccagtgg
    58201 aaacggtgat aaagaccata tcggttatat cgccttccag cgcctgatac tctgcccatg
    58261 ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt
    58321 tgactaacgg aaagtgtagc aatccaccga gattatgcgg attcagcgcc agcaaactgc
    58381 cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag
    58441 agactaatgc ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa
    58501 gttcacgacg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac
    58561 cgccgatatc aatcgccagc gtggtcatag caccgccttt ttcatcgctg tgttgtacca
    58621 ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg
    58681 gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcaccctaca
    58741 tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt
    58801 ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc
    58861 gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag
    58921 cagcgtttca acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc
    58981 gcccgcctgc gccagcgcat caacatcttc aatataggcc gtgatgcgta ccggagaatc
    59041 ctccagatcg cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg
    59101 cagatttgcc acaccttcaa tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc
    59161 catggcggcg acgatttcgg gtttatcgag cgggctgtcc ggaaccggct ggcaggagac
    59221 aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct
    59281 tcccttagcg aaaggcccgg tacatagacc gggcaacagg attaactttt ggttttgact
    59341 aaatcgtttt tggcgctgcc aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg
    59401 tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa cgcgggaagg catatccaga
    59461 ccaatcagca ggatcaccac aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc
    59521 agatccagac gttgagcgat caacgcgccg atgattgggg ccagtgcgcc gcccaatgca
    59581 ccaacgttgt aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca
    59641 atcagtttcg gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac
    59701 agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat caacagctgc
    59761 gaggccaaca ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagccg
    59821 ccgacacagc accccaccgc agccccaaag ccgctaaaga acagcacatt ggcgacagta
    59881 tgcgggttat aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat
    59941 gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgttta
    60001 cccgtacttt gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg
    60061 acgatcgcgg cattttgcag gttaccggcg aagcaaaacc acagcgcagt agccgccgcc
    60121 agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatatc taccatcgtg
    60181 cgtactggtg ctttacctgc atgtttctct ttccagtctt ccgcttccgg gatgttttta
    60241 cgcagccaga gagcaaagat gataggcaaa atgccgataa agaacagcgc acgccagccc
    60301 cagaccggaa ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct
    60361 gaaatcagaa aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag
    60421 gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac
    60481 atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg
    60541 ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg
    60601 ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc
    60661 ccgaattcac cttgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca
    60721 agcagatatc ccaaccaggc agcggaaaat gcccgccatt gtgcacgatt gagatggcga
    60781 taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc
    60841 acacgctttg gtatgaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg
    60901 ggcgagcgag gggaaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    60961 cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga
    61021 aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    61081 taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga tatcgccttc
    61141 tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg tactaccgat
    61201 accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    61261 cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaagtctg
    61321 tttcagcgca ccgacgccag gcaacgtaac cagggtgttg atctgatcca gggtcagttt
    61381 taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg ctgaatcaat
    61441 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    61501 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    61561 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    61621 gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc
    61681 acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc gacgcagact
    61741 cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca ttacgccacg
    61801 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    61861 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    61921 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag
    61981 cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc
    62041 ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg
    62101 cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg
    62161 ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac
    62221 gaatgaatgc cgcgttgtta tccagcgact gactgttaat ttccagtgct tttgccagca
    62281 aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccaggctg gattcaaaga
    62341 acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca
    62401 tgccggaaag ctcgccgatg atggtgtccg cagaaggacg tgagacgcgg gcacgttcgc
    62461 cgttgttgat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttcg cgcacagaag
    62521 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc
    62581 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    62641 tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt
    62701 gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt
    62761 gacatcctat cacaggattg aaagtagggg aaaatggcag gtttttctct ttgtgcctca
    62821 tcattaccat aattaacgga ataattaact attgcgaaaa attaatgtaa cgcagataaa
    62881 aacatcccgt ttgaattatt tat
//
LOCUS       DAZKGS010000008        59231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKGS010000008 DAZKGS010000000
VERSION     DAZKGS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..533
                     /gene="alaE"
                     /locus_tag="ACHOQY_000524"
     CDS             84..533
                     /gene="alaE"
                     /locus_tag="ACHOQY_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9676681.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(570..914)
                     /gene="ygaC"
                     /locus_tag="ACHOQY_000525"
     CDS             complement(570..914)
                     /gene="ygaC"
                     /locus_tag="ACHOQY_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9676682.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1066..1395
                     /gene="ygaM"
                     /locus_tag="ACHOQY_000526"
     CDS             1066..1395
                     /gene="ygaM"
                     /locus_tag="ACHOQY_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9676683.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1643..1888
                     /gene="nrdH"
                     /locus_tag="ACHOQY_000527"
     CDS             1643..1888
                     /gene="nrdH"
                     /locus_tag="ACHOQY_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9676684.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1885..2295
                     /gene="nrdI"
                     /locus_tag="ACHOQY_000528"
     CDS             1885..2295
                     /gene="nrdI"
                     /locus_tag="ACHOQY_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9676685.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2268..4412
                     /gene="nrdE"
                     /locus_tag="ACHOQY_000529"
     CDS             2268..4412
                     /gene="nrdE"
                     /locus_tag="ACHOQY_000529"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9676686.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4422..5381
                     /gene="nrdF"
                     /locus_tag="ACHOQY_000530"
     CDS             4422..5381
                     /gene="nrdF"
                     /locus_tag="ACHOQY_000530"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9676687.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5736..6938
                     /gene="proV"
                     /locus_tag="ACHOQY_000531"
     CDS             5736..6938
                     /gene="proV"
                     /locus_tag="ACHOQY_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9676688.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6931..7995
                     /gene="proW"
                     /locus_tag="ACHOQY_000532"
     CDS             6931..7995
                     /gene="proW"
                     /locus_tag="ACHOQY_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9676689.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8053..9045
                     /gene="proX"
                     /locus_tag="ACHOQY_000533"
     CDS             8053..9045
                     /gene="proX"
                     /locus_tag="ACHOQY_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9676690.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9237..10421
                     /locus_tag="ACHOQY_000534"
     CDS             9237..10421
                     /locus_tag="ACHOQY_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9676691.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10545..11282
                     /gene="ygaZ"
                     /locus_tag="ACHOQY_000535"
     CDS             10545..11282
                     /gene="ygaZ"
                     /locus_tag="ACHOQY_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9676692.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11272..11607
                     /gene="ygaH"
                     /locus_tag="ACHOQY_000536"
     CDS             11272..11607
                     /gene="ygaH"
                     /locus_tag="ACHOQY_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9676693.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11698..12228
                     /gene="emrR"
                     /locus_tag="ACHOQY_000537"
     CDS             11698..12228
                     /gene="emrR"
                     /locus_tag="ACHOQY_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9676694.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12355..13527
                     /gene="emrA"
                     /locus_tag="ACHOQY_000538"
     CDS             12355..13527
                     /gene="emrA"
                     /locus_tag="ACHOQY_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9676695.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13544..15082
                     /gene="emrB"
                     /locus_tag="ACHOQY_000539"
     CDS             13544..15082
                     /gene="emrB"
                     /locus_tag="ACHOQY_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9676696.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15146..15661)
                     /gene="luxS"
                     /locus_tag="ACHOQY_000540"
     CDS             complement(15146..15661)
                     /gene="luxS"
                     /locus_tag="ACHOQY_000540"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9676697.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15811..17367)
                     /gene="gshA"
                     /locus_tag="ACHOQY_000541"
     CDS             complement(15811..17367)
                     /gene="gshA"
                     /locus_tag="ACHOQY_000541"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9676698.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(17440..17868)
                     /gene="yqaA"
                     /locus_tag="ACHOQY_000542"
     CDS             complement(17440..17868)
                     /gene="yqaA"
                     /locus_tag="ACHOQY_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9676699.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17865..18431)
                     /gene="yqaB"
                     /locus_tag="ACHOQY_000543"
     CDS             complement(17865..18431)
                     /gene="yqaB"
                     /locus_tag="ACHOQY_000543"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9676700.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18579..18655)
                     /locus_tag="ACHOQY_000544"
     tRNA            complement(18579..18655)
                     /locus_tag="ACHOQY_000544"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18619..18621),aa:Arg,seq:acg)
     gene            complement(18854..18930)
                     /locus_tag="ACHOQY_000545"
     tRNA            complement(18854..18930)
                     /locus_tag="ACHOQY_000545"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18894..18896),aa:Arg,seq:acg)
     gene            complement(18994..19070)
                     /locus_tag="ACHOQY_000546"
     tRNA            complement(18994..19070)
                     /locus_tag="ACHOQY_000546"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19034..19036),aa:Arg,seq:acg)
     gene            complement(19074..19166)
                     /locus_tag="ACHOQY_000547"
     tRNA            complement(19074..19166)
                     /locus_tag="ACHOQY_000547"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19130..19132),aa:Ser,seq:gct)
     gene            complement(19482..19667)
                     /gene="csrA"
                     /locus_tag="ACHOQY_000548"
     CDS             complement(19482..19667)
                     /gene="csrA"
                     /locus_tag="ACHOQY_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9676701.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(19902..22532)
                     /gene="alaS"
                     /locus_tag="ACHOQY_000549"
     CDS             complement(19902..22532)
                     /gene="alaS"
                     /locus_tag="ACHOQY_000549"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9676702.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(22660..23160)
                     /gene="recX"
                     /locus_tag="ACHOQY_000550"
     CDS             complement(22660..23160)
                     /gene="recX"
                     /locus_tag="ACHOQY_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9676703.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23403..24464)
                     /gene="recA"
                     /locus_tag="ACHOQY_000551"
     CDS             complement(23403..24464)
                     /gene="recA"
                     /locus_tag="ACHOQY_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9676704.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(24544..25041)
                     /gene="pncC"
                     /locus_tag="ACHOQY_000552"
     CDS             complement(24544..25041)
                     /gene="pncC"
                     /locus_tag="ACHOQY_000552"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9676705.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25186..26271)
                     /gene="mltB"
                     /locus_tag="ACHOQY_000553"
     CDS             complement(25186..26271)
                     /gene="mltB"
                     /locus_tag="ACHOQY_000553"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9676706.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            26527..27090
                     /gene="srlA"
                     /locus_tag="ACHOQY_000554"
                     /gene_synonym="gutA"
     CDS             26527..27090
                     /gene="srlA"
                     /locus_tag="ACHOQY_000554"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9676707.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27087..28046
                     /gene="srlE"
                     /locus_tag="ACHOQY_000555"
                     /gene_synonym="gutE"
     CDS             27087..28046
                     /gene="srlE"
                     /locus_tag="ACHOQY_000555"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9676708.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            28057..28428
                     /gene="srlB"
                     /locus_tag="ACHOQY_000556"
                     /gene_synonym="gutB"
     CDS             28057..28428
                     /gene="srlB"
                     /locus_tag="ACHOQY_000556"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9676709.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28432..29211
                     /gene="srlD"
                     /locus_tag="ACHOQY_000557"
     CDS             28432..29211
                     /gene="srlD"
                     /locus_tag="ACHOQY_000557"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9676710.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            29316..29675
                     /gene="gutM"
                     /locus_tag="ACHOQY_000558"
     CDS             29316..29675
                     /gene="gutM"
                     /locus_tag="ACHOQY_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9676711.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            29742..30515
                     /gene="srlR"
                     /locus_tag="ACHOQY_000559"
                     /gene_synonym="gutR"
     CDS             29742..30515
                     /gene="srlR"
                     /locus_tag="ACHOQY_000559"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9676712.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            30508..31473
                     /gene="gutQ"
                     /locus_tag="ACHOQY_000560"
     CDS             30508..31473
                     /gene="gutQ"
                     /locus_tag="ACHOQY_000560"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9676713.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31470..32984)
                     /gene="norR"
                     /locus_tag="ACHOQY_000561"
     CDS             complement(31470..32984)
                     /gene="norR"
                     /locus_tag="ACHOQY_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9676714.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            33171..34610
                     /gene="norV"
                     /locus_tag="ACHOQY_000562"
     CDS             33171..34610
                     /gene="norV"
                     /locus_tag="ACHOQY_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9676715.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            34607..35740
                     /gene="norW"
                     /locus_tag="ACHOQY_000563"
     CDS             34607..35740
                     /gene="norW"
                     /locus_tag="ACHOQY_000563"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9676716.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(35966..38218)
                     /gene="hypF"
                     /locus_tag="ACHOQY_000564"
     CDS             complement(35966..38218)
                     /gene="hypF"
                     /locus_tag="ACHOQY_000564"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9676717.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38371..38898)
                     /gene="hydN"
                     /locus_tag="ACHOQY_000565"
     CDS             complement(38371..38898)
                     /gene="hydN"
                     /locus_tag="ACHOQY_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9676718.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39047..40057)
                     /gene="ascG"
                     /locus_tag="ACHOQY_000566"
     CDS             complement(39047..40057)
                     /gene="ascG"
                     /locus_tag="ACHOQY_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9676719.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            40317..41774
                     /gene="ascF"
                     /locus_tag="ACHOQY_000567"
     CDS             40317..41774
                     /gene="ascF"
                     /locus_tag="ACHOQY_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP9676720.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            41783..43207
                     /gene="ascB"
                     /locus_tag="ACHOQY_000568"
     CDS             41783..43207
                     /gene="ascB"
                     /locus_tag="ACHOQY_000568"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9676721.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(43321..43740)
                     /locus_tag="ACHOQY_000569"
     CDS             complement(43321..43740)
                     /locus_tag="ACHOQY_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9676722.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(43737..44048)
                     /locus_tag="ACHOQY_000570"
     CDS             complement(43737..44048)
                     /locus_tag="ACHOQY_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP9676723.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(44222..44965)
                     /locus_tag="ACHOQY_000571"
     CDS             complement(44222..44965)
                     /locus_tag="ACHOQY_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676724.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(45008..45478)
                     /gene="hycI"
                     /locus_tag="ACHOQY_000572"
     CDS             complement(45008..45478)
                     /gene="hycI"
                     /locus_tag="ACHOQY_000572"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9676725.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(45471..45881)
                     /gene="hycH"
                     /locus_tag="ACHOQY_000573"
     CDS             complement(45471..45881)
                     /gene="hycH"
                     /locus_tag="ACHOQY_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9676726.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(45878..46645)
                     /gene="hycG"
                     /locus_tag="ACHOQY_000574"
     CDS             complement(45878..46645)
                     /gene="hycG"
                     /locus_tag="ACHOQY_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9676727.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(46645..47187)
                     /gene="hycF"
                     /locus_tag="ACHOQY_000575"
     CDS             complement(46645..47187)
                     /gene="hycF"
                     /locus_tag="ACHOQY_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9676728.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47197..48906)
                     /gene="hycE"
                     /locus_tag="ACHOQY_000576"
     CDS             complement(47197..48906)
                     /gene="hycE"
                     /locus_tag="ACHOQY_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9676729.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(48924..49847)
                     /gene="hycD"
                     /locus_tag="ACHOQY_000577"
     CDS             complement(48924..49847)
                     /gene="hycD"
                     /locus_tag="ACHOQY_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9676730.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(49850..51676)
                     /gene="hycC"
                     /locus_tag="ACHOQY_000578"
     CDS             complement(49850..51676)
                     /gene="hycC"
                     /locus_tag="ACHOQY_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9676731.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(51673..52284)
                     /gene="hycB"
                     /locus_tag="ACHOQY_000579"
     CDS             complement(51673..52284)
                     /gene="hycB"
                     /locus_tag="ACHOQY_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9676732.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(52409..52870)
                     /gene="hycA"
                     /locus_tag="ACHOQY_000580"
     CDS             complement(52409..52870)
                     /gene="hycA"
                     /locus_tag="ACHOQY_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9676733.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53082..53432
                     /gene="hypA"
                     /locus_tag="ACHOQY_000581"
     CDS             53082..53432
                     /gene="hypA"
                     /locus_tag="ACHOQY_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9676734.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            53436..54308
                     /gene="hypB"
                     /locus_tag="ACHOQY_000582"
     CDS             53436..54308
                     /gene="hypB"
                     /locus_tag="ACHOQY_000582"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9676735.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54299..54571
                     /gene="hypC"
                     /locus_tag="ACHOQY_000583"
     CDS             54299..54571
                     /gene="hypC"
                     /locus_tag="ACHOQY_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9676736.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            54571..55692
                     /gene="hypD"
                     /locus_tag="ACHOQY_000584"
     CDS             54571..55692
                     /gene="hypD"
                     /locus_tag="ACHOQY_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9676737.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            55689..56699
                     /gene="hypE"
                     /locus_tag="ACHOQY_000585"
     CDS             55689..56699
                     /gene="hypE"
                     /locus_tag="ACHOQY_000585"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9676738.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            56773..58841
                     /gene="flhA"
                     /locus_tag="ACHOQY_000586"
                     /pseudo
     CDS             56773..58841
                     /gene="flhA"
                     /locus_tag="ACHOQY_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(58878..59231)
                     /gene="ygbA"
                     /locus_tag="ACHOQY_000587"
     CDS             complement(58878..59231)
                     /gene="ygbA"
                     /locus_tag="ACHOQY_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9676739.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        13946 a        16030 c        15290 g        13965 t
ORIGIN      
        1 tatgcgaaaa tgcagatggc aatgagatcc actgctttca tctccattaa catcccatta
       61 cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg
      121 cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc
      181 tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggcgaaca
      241 tcttaattgc atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg gcacgcaaag
      301 ttagcccgtc gggctggata aaaaatctgg ctgatatcct ggcttatgtg acgttccagt
      361 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
      421 cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
      481 attattgccg ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct
      541 tttcgcgact ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa
      601 taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc
      661 tcgcctgcta aatagagcgg aaattgctgg taatgatcgc aagttttaat atcggaagcg
      721 ggttcagcca gtgttgaact gcgttctttt aatgttggat gaattaccag agcggtacgt
      781 cccattcgcg cttcgcgatt aacatagaca taattttccc cacggcggta accatacgct
      841 ttctgggtta ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct
      901 ggtcgtaaat acatatttga tcctcgttat tatcaaccgc gggcctacct tacctgattg
      961 cgcattcaca acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat
     1021 tatggtctac actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg
     1081 aaccgcaacg acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct
     1141 gacagcctcg aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga agctgaagcc
     1201 gcacgcagta aagctcaggc attgctgaaa gaaaccaggg cgcgaatgca tggtcgtact
     1261 cgcgtccagc aagccgcacg cgatgccgtt ggctgcgcgg attcttttgt tcgtgaaaga
     1321 ccctggtgta gcgtgggtac agcagctgcg gtaggtattt ttatcggcgc actgttaagc
     1381 atgcgcaaat cgtagtgcaa aaatgataat aaatacgcgt ctttgacccc gaagcctggc
     1441 ttcggggttt ctttttgcct ggtgaatcac aaaaatcccc ctaccccgtc acgctcatat
     1501 ccagggtaat ttcgaccact atttgctata tattgtgtgg ttgaatcttt tttcaactac
     1561 atctagtatc tctgtatcaa cagagagaca acccgacgcg tatcatcgcg ccgtatcttc
     1621 attttaaacg gaaatacgaa tcatgcgcat tactatttac actcgtaacg attgcgttca
     1681 gtgccacgcc accaaacggg cgatggaaaa ccggggcttt gattttgaaa tgattaatgt
     1741 cgatcgcgtt cctgaagcag cggaagcgtt gcgtgctcag ggctttcgtc agttgccggt
     1801 agtgattgct ggcgatctta gctggtctgg tttccgtccg gacatgatta accgtctgca
     1861 tccagcgcca cacgcggcca gtgcatgagc cagctcgtct acttctccag cagctccgaa
     1921 aacacgcagc gttttatcga acgtttaggt ctgcccgcgg tgcgcatccc gctcaatgag
     1981 cgggaacgga ttcaggtaga cgagccttac atcctgatcg tgccctctta cggcggcggc
     2041 ggtacggctg gcgcggtgcc acgacaggta attcgctttt taaacgacga gcacaaccgg
     2101 gcgttgcttc gcggcgttat tgcttctggt aatcgcaact ttggtgaggc gtatggccgc
     2161 gccggagatg tgattgcccg gaaatgcggc gtgccgtggc tgtaccgttt tgaactcatg
     2221 ggtacgcaaa gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg gcaacgacaa
     2281 ccgcagaatg cctgacgcag gaaacgatgg attaccacgc gctgaatgcg atgcttaacc
     2341 tctacgatag cgcaggtcgc attcagttcg ataaagaccg ccaggccgtt gacgccttta
     2401 ttgcgacgca tgtgcgtccg aacagtgtga ccttcagtag ccagcagcag cgcctgaact
     2461 ggctggtcaa cgaaggttac tatgatgaaa gcgttcttaa tcgctactct cgcgactttg
     2521 tcattacgct gtttgcccac gcacacacca gcggttttcg tttccagaca ttcctcgggg
     2581 catggaagtt ttacaccagc tatacgttga agacattcga cggtaaacgt tatctggaag
     2641 attttgccga tcgagtcacg atggtggcgc tgacgctggc acaaggcgat gcgacgctgg
     2701 cgttgcaact gaccgatgaa atgctgtcag gacgctttca gccagccacg ccaacattcc
     2761 tcaactgcgg taagcagcag cgcggcgaac tggtttcctg ttttctgctg cgtattgaag
     2821 acaatatgga gtcgattggc cgggcggtaa attccgcact gcaactgtcg aaacgcggcg
     2881 gcggcgtggc atttttgctg tcgaatctgc gggaagcggg cgcgccaatt aaacgtattg
     2941 aaaatcaatc ttctggcgta attccggtga tgaaaatgct ggaagacgca ttttcctatc
     3001 ccaaccaact cggcgcgcgt cagggggctg gtgcagtcta tttacatgct catcatcccg
     3061 atattctgcg ttttctcgac acgaaacggg aaaatgccga cgaaaaaatc cgcattaaaa
     3121 cactgtcgct tggcgtggtg atcccggata tcactttcca tctggcaaaa gagaatgcgc
     3181 agatggcgct gttttcgccc tatgacgttg agcgagttta tggcaagccg tttgccgata
     3241 tcgccattag cgaacactat gacgaactgg ttgccgatga acgcatccgc aaaaaatacc
     3301 tcaacgcccg tgatttcttc cagcgactgg cagaaatcca gtttgagtcc ggctatccct
     3361 acatcatgta tgaagacacg gtaaaccgtg ctaaccctat cgccgggcgc ataaatatga
     3421 gtaatctctg ctcagaaatt ttgcaggtta acagcgcctc agagtatgac gagaatctcg
     3481 actatgcccg tacaggccat gatatttcct gcaatttagg ttcgttgaat attgcccaca
     3541 ccatggattc ccccaacttt gcccgcacgg tagagactgc cgtacgcggt ttaacggcgg
     3601 tgtcagatat gagtcatatc cgcagcgtgc cgtccatcga agccggaaat gccgcctcgc
     3661 acgccatcgg actggggcag atgaatttac atggttatct ggcgagggaa ggcatcgctt
     3721 atggttcgcc ggaagccctg gatttcacca atctctattt ctataccatc acctggcacg
     3781 cactgcgtac ctcgatgttg ctggcgcgcg aacgcggtga aaccttcgcc gggttcaaac
     3841 agtcacgcta tgccagtggt gaatatttca gccaatatct gcaaggtaac tggcagccga
     3901 aaacggcgaa agttggcgaa ctgtttgccc gcagcggtat tacgttacct acccgtgaga
     3961 tgtgggcgca actgcgcgac gacgtgatgc gctacggcat atacaaccag aatctccagg
     4021 cggtgccgcc aaccggttct atctcttata tcaaccatgc tacgtcgagt attcatccga
     4081 ttgtggcgaa agtagagata cgcaaagagg gcaaaacagg acgcgtttac taccctgccc
     4141 cgtttatgac taacgagaat ctggcgctgt atcaggatgc ttacgaaatc ggcgcagaaa
     4201 agatcatcga cacctacgcg gaagcgactc gccatgtcga tcagggtttg tcgctgacgc
     4261 tgtttttccc cgataccgcc accactcgcg atatcaacaa agcgcagatt tacgcctggc
     4321 gcaaaggtat caaaacgctc tattacattc gcctgcgtca gatggcgctg gaaggcactg
     4381 aaattgaagg ctgcgtctcc tgtgcacttt aaggaatatc tatgaaactc tcacgtatca
     4441 gcgccatcaa ctggaacaag atatctgacg ataaagatct ggaggtgtgg aatcgcctga
     4501 ccagcaattt ctggctacca gaaaaggtgc cactgtcgaa cgatatacct gcatggcaga
     4561 cattaagtgt cgtagaacaa caactgacaa tgcgcgtttt tactggcctg acgctgctcg
     4621 acacgctgca aaatgttatc ggcgcgcctt cgctgatgcc cgatgcgctc acgcctcatg
     4681 aagaggcagt attatcgaat atcagcttta tggaagcggt tcatgcccgc tcttacagtt
     4741 cgattttctc gacgctgtgt cagaccaaag atgtcgatgc cgcctacgcc tggagtgaag
     4801 aaaacgcacc gttgcagcga aaagctcaga ttattcagca acattatcgc ggtgatgatc
     4861 cgctgaaaaa gaaaatcgcc agtgtgtttc ttgaatcttt tttgttctat tccggtttct
     4921 ggctgccgat gtatttttcc agccgcggaa agctgaccaa taccgcagat ctgatccgtc
     4981 tgattatccg cgatgaagca gtccacggtt actacatagg ctataagtat cagaaaaaca
     5041 tggaaaagat atctctggga caacgtgaag agttgaagag tttcgccttc gatttgttgc
     5101 tggaactcta cgacaacgag ttgcaataca ccgatgagct gtacgccgaa accccgtggg
     5161 ctgacgatgt gaaagcgttt ctctgttaca acgccaataa ggctttgatg aatctgggct
     5221 acgaaccgtt atttcccgca gaaatggcgg aagtgaatcc ggcaatcctc gccgcgcttt
     5281 cgccgaatgc cgatgaaaat cacgatttct tttccggttc aggctcctct tatgtgatgg
     5341 ggaaagcggt tgaaacagaa gatgaagact ggaatttctg agggtgttat tttcaaaaat
     5401 atcactaccc gcagcaggga aataattccc gccaaatagc tttttatcac gcaaatattc
     5461 tgtagtgatc tacactgata ctctgttgca ttattcgcct gaaactacaa tattcaggcg
     5521 ttttttcgct atctatgaca aaaaatatca acttttctcg atttgctctc agcccttata
     5581 tcacgggaaa ttccggcgat ttgctcgcat caatattcat gccacatttg ccctcagggg
     5641 ttgcctcaga ttctcagtat gttagggtag aaaaaagtga ctatttccat tgggtaatat
     5701 atcgacataa acaaataaag gaatctttct attgcatggc aattaaatta gaaattaaaa
     5761 atctttataa aatatttggc gagcatccac agcgagcgtt caaatatatc gaacaaggac
     5821 tttcgaaaga acaaattctg gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc
     5881 tggccattga agaaggcgag atatttgtca tcatgggatt atccggctcg ggtaaatcca
     5941 caatggtacg ccttctcaat cgcctgattg aacccacccg cgggcaagtg ctgattgatg
     6001 gtgtggatat tgccaaaata tccgatgccg aactccgtga ggtgcgcaga aaaaagattg
     6061 cgatggtctt ccagtccttt gccttaatgc cgcatatgac cgtgctggac aatactgcgt
     6121 tcggtatgga attggccgga attaatgccg aagaacgccg ggaaaaagcc cttgatgcac
     6181 tgcgtcaggt cgggctggaa aattatgccc acagctaccc ggatgaactc tctggcggga
     6241 tgcgtcaacg tgtgggatta gcccgcgcgt tagcgattaa tccggatata ttattaatgg
     6301 acgaagcctt ctcggcgctc gatccattaa ttcgcaccga gatgcaggat gagctggtaa
     6361 aattacaggc gaaacatcag cgcaccattg tctttatttc ccacgatctc gatgaggcta
     6421 tgcgtattgg cgaccgaatt gccattatgc aaaatggtga agtggtacag gtcggcacac
     6481 cggatgaaat tctcaataat ccggcgaatg attatgtccg taccttcttc cgtggcgttg
     6541 atattagtca ggtattcagt gcgaaagata ttgcccgccg gacaccgaat ggcttaattc
     6601 gtaaaacccc tggcttcggc ccacgttcgg cactgaaatt attgcaggat gaagatcgcg
     6661 aatatggcta cgttatcgaa cgcggtaata agtttgtcgg cgcagtttcc atcgattcgc
     6721 ttaaaaccgc gttaacgcag cagcaaggtc ttgatgcggc gctgattgat gcgccgttag
     6781 cagtcgatgc acaaacgcct cttagcgagt tgctctctca tgtcggacag gcaccctgtg
     6841 cggtgcccgt ggtcgacgag gaccaacagt atgtcggcat catttcgaaa ggaatgctgc
     6901 tgcgcgcttt agatcgtgag ggggtaaata atggctgatc aaaataatcc gtgggatacc
     6961 acgccagcgg cggacagtgc cgtgcaatcc gcagacgcct ggggtacacc ggcgactgca
     7021 ccgactgacg gcggtggtgc tgactggctg accagtacgc ctgcgccaaa cgtcgagcat
     7081 tttaatattc tcgatccgtt ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa
     7141 gggatcgact gggttgttac ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt
     7201 gattacattc tcaacggttt ccagcaattg ctgctgggta tgcccgcacc ggtggcgatt
     7261 atcgttttcg ctctcatcgc ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg
     7321 gtttcgctga ttgccatcgg cgcaatcggt gcctggtcgc aggccatggt taccctggcg
     7381 ctggtgttaa ccgccctgct gttctgtatc gtcattggtt tgccgctggg gatctggctg
     7441 gcgagaagtc cgcgagcggc gaaaattatt cgtccactgc ttgatgccat gcagaccacg
     7501 ccagcgtttg tttatctggt gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc
     7561 gtggtggtga cgatcatctt tgctctgccg ccgattatcc gtctgaccat tctggggatt
     7621 aaccaggttc cggcggatct gattgaagcc tcgcgctcat tcggtgccag cccgcgccag
     7681 atgctgttca aagttcagtt accgctggcg atgccgacca ttatggcggg cgttaaccag
     7741 acgctgatgc tggccctttc tatggtggtc attgcctcga tgattgccgt cggcgggctg
     7801 ggtcagatgg tacttcgcgg tatcggtcgt ctggatatgg ggcttgccac cgttggcggc
     7861 gtcgggattg tgatcctcgc cattatcctc gatcgcctga cgcaggccgt tgggcgcgac
     7921 tcacgcagtc gcggcaaccg tcgctggtac accactggcc ctgttggtct gctgacccgc
     7981 ccattcatta agtaactctg cacttgcccg gtgacgccgg gcattatcac cctgccaaaa
     8041 aaaggaataa caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct
     8101 acacaaactt ttgctgccga tctgccgggc aaaggcatta ctgttaatcc agttcagagc
     8161 accatcactg aagaaacctt ccagacgctg ctggtcagtc gtgcgctgga gaaattaggt
     8221 tataccgtca acaaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc
     8281 ggcgatgcaa ccttcaccgc cgtgaactgg acgccactgc atgacaacat gtacgaagct
     8341 gccggtggcg ataagaaatt ttatcgtgaa ggggtatttg ttaacggcgc ggcacagggt
     8401 tacctgatcg ataagaaaac cgccgaccag tacaaaatca ccaacatcgc acaactgaaa
     8461 gatccgaaga tcgccaaact gttcgatacc aacggcgacg gaaaagcgga tttaaccggt
     8521 tgtaaccctg gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatgaactg
     8581 acccataccg tgacgcataa tcaggggaac tacgcggcga tgatggccga caccatcagt
     8641 cgctacaaag agggcaaacc ggtgttttac tacacctgga cgccgtactg ggtgagtaat
     8701 gagctgaagc cagggaaaga tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc
     8761 gataaaaacg ccgataccaa actgccgaat ggtgcgaatt atggcttccc ggtcagcacc
     8821 atgcatatcg ttgccaacaa agcctgggcc gagaaaaacc cggcagcagc gaaactgttt
     8881 gccattatgc agttgccagt ggcagatatt aacgcccaga acgccattat gcatgacggc
     8941 aaagcctcag aaggcgatat tcagggccat gttgatggct ggatcaaagc ccaccagcag
     9001 cagttcgatg gctgggtgaa tgaggcgctg gcagcgcaga agtaattttt attcgggcgg
     9061 ataaggcgtt tttgcctcat ccgccgctct gtacacaatg cctgatgcga cgctgacgcg
     9121 tcttatcatg cctacaaacc acatcgcaca tttcaacaag ctatctttca tcccatattc
     9181 atcaacatcc gctattattg atttccagct taatcatcac ctgatgaaca aaaataatga
     9241 ctaaacctaa tcatgagctt agcccggcgc tgatcgtgct gatgtctatc gccaccggtc
     9301 tggcggtcgc cagtaactat tacgcccagc cgctactcga caccatcgcg cgtaactttt
     9361 ccctttccgc cagttcggca ggctttattg ttaccgccgc gcaattgggc tatgcagcag
     9421 gtctgctgtt tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg attgtctcga
     9481 tgaccttact ggcagcgggc ggtatgttga ttaccgccag cagtcagtcg ctggcgatga
     9541 tgctcctcgg tacggcatta accggtttat tctctgtcgt ggcacaaatt ctggttccgc
     9601 tggcagcgac gctggcttca ccggataaac gcggcaaagt ggttggcacc attatgagcg
     9661 gtctgctgtt ggggatattg ctggcacgaa ccgttgccgg gttactggca aacctcggcg
     9721 gctggcgcac cgtcttttgg gttgcctcgg tgttaatggc tctgatggcg ctagcattat
     9781 ggcgtggtct gccacaaatg aaatcagaaa cccacctcaa ctacccacag ttgttgggtt
     9841 ccgttttcag catgtttatc aacgataaga tcctgcgcac ccgcgcattg cttggctgcc
     9901 tgacctttgc caacttcagc attctctgga cctcaatggc ctttttgctt gccgctccac
     9961 cttttaacta cagcgatggt gtcattggtc tgtttggact tgcgggagct gccggcgcgt
    10021 tgggcgctcg tccggcgggc ggttttgccg ataagggtaa atcgcaccac accacaactt
    10081 tcggtctgct gctgctatta ctttcatggc tggcgatctg gtttggtcac acttccgtac
    10141 tggcgttgat tatcggaatc ctggtgctgg atctcaccgt acagggcgtg catatcacta
    10201 accagacggt aatttatcga atacaccctg atgcgcgtaa tcgcctgacc gcaggttaca
    10261 tgaccagcta ctttattggc ggtgccgccg gttcgctaat ttcagcctca gcctggcaac
    10321 atggcggttg ggctggcgtt tgtctggctg gcgcgacgat tgccctggtt aacttactgg
    10381 tctggtggcg aggttttcat cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc
    10441 gtggctttac acttataagg gtgttaagaa gcccatcagt ctgataaggt taagatattc
    10501 attcagtcta tttataatat taacaatcgt taagcgtaca ctctatggaa agccctactc
    10561 cacagcctgc tcctggttcg gcgaccttca tggaaggatg caaagacagt ttaccgattg
    10621 ttattagtta tattccggtg gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct
    10681 ctcctctcga aagcgttttt ttctcctgca tcatttatgc aggcgcgagc cagttcgtca
    10741 ttaccgcgat gctggcagcc gggagtagtt tgtgggttgc tgcactgacc gtcatggcaa
    10801 tggatgttcg ccatgtgttg tatggcccgt cactgcgtag ccgtattatt cagcgtctgc
    10861 aaaaatcgaa aaccgccctg tgggcatttg gcctgacgga tgaggttttt gccgccgcaa
    10921 ccgcaaaact ggtacgcaat aatcgccgct ggagcgagaa ctggatgatc ggcattgcct
    10981 tcagttcatg gtcatcgtgg gtatttggta cggtaatagg ggcattctcc ggcagcggct
    11041 tgctgcaagg ttatcccgcc gttgaagctg cattaggttt tatgcttccg gcactcttta
    11101 tgagtttcct gctcgcctct ttccagcgca aacaatctct ttgcgttacc gcagcgttag
    11161 ttggtgccct tgcaggcgta acgctatttt ctattcccgt cgccattctg gcaggcattg
    11221 tctgtggctg cctcactgcg ttaatccagg cattctggca aggagcgccc gatgagctat
    11281 gaggttctgc tgcttgggtt actggttggc gtggcgaatt attgcttccg ctatttgccg
    11341 ctgcgcctgc gtgtgggtaa tgcccgccca accaaacgtg gcgcggtagg tattttgctc
    11401 gacaccattg gcatcgcctc gatatgcgct ctgctggttg tctctaccgc accagaagtg
    11461 atgcacgata cacgccgttt cgtgcccacg ctggtcggct tcgcggtact gggtgccagt
    11521 ttctataaaa cacgcagcat tatcatccca acactgctta gtgcgctggc ctatgggctc
    11581 gcctggaaag tgatggcgat tatataaccc acaagaatca tttttctaaa acaatacatt
    11641 tactttattt gtcactgtcg ttactatatc ggctgaaatt aatgaggtca tacccaaatg
    11701 gatagttcgt ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat
    11761 tttccttatc aggagatcct tctgactcgt ctttgcatgc acatgcaaag caagctgctg
    11821 gagaaccgca ataaaatgct gaaggctcag gggattaacg agacgttgtt tatggcgttg
    11881 attacgctgg agtctcagga aaaccacagt attcagcctt ctgaattaag ttgtgctctt
    11941 ggatcatccc gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg cggttggatc
    12001 gaacgtcgtg aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt
    12061 cacgagtttt tgcgcgaggt tttaccaccg cagcataact gcctgcatca actctggtcc
    12121 gcgctcagca caacagaaaa agatcagctc gagcaaatca cccgcaaatt gctctcccgt
    12181 ctcgaccaga tggaacaaga cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg
    12241 ctcaaaaatc cagatttata aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct
    12301 ttttggcaag cagctcggct cagccgatga gttaagaaga tcgtggagaa caatatgagc
    12361 gcaaatgcgg agactcaaac cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt
    12421 ctgctcctcc ttctcacctt gctctttata attattgccg tagcgatagg gatttattgg
    12481 tttttggtac tgcgtcattt cgaagaaacc gatgacgcat acgtggcagg gaatcagatt
    12541 caaattatgt ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa caccgatttt
    12601 gtaaaagaag gcgatgttct ggtcactctc gacccgacag atgctcgcca ggcgtttgaa
    12661 aaagccaaaa ctgcactggc ttccagcgtt cgccaaaccc accagctgat gattaacagc
    12721 aaacagttgc aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac
    12781 tacaaccgcc gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac
    12841 gcccgcgacg ccgtcaccag tgcccaggcg caactggacg tcgctattca acaatacaat
    12901 gccaatcagg cgatgattct ggggactaaa ctggaagatc agcctgccgt gcaacaggct
    12961 gccaccgaag tacgtaacgc ctggctggcg ctggagcgta cccgtattgt cagtccgatg
    13021 accggttatg tctcccgccg cgcggtacag cctggggcgc aaattagccc aacgacgccg
    13081 ctgatggcgg tcgttccagc caccaatatg tgggtggatg ccaactttaa agagacgcag
    13141 attgccaata tgcgtatcgg tcagccggtc actatcacca cggatattta cggcgatgat
    13201 gtgaaataca ccggtaaagt ggttggtctg gatatgggca caggtagcgc attctcactg
    13261 cttccggcgc aaaatgcgac cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt
    13321 atcgaactgg accagaaaca gctggagcaa tacccgctgc gcattggttt gtccacgctg
    13381 gtgagcgtca ataccactaa ccgtgacggt caggtactgg caaataaagt acgttcaaca
    13441 ccggtcgcgg taagtaccgc gcgtgaaatc agcctggcac ctgtcaataa actgatcgac
    13501 gatatcgtaa aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc
    13561 gctggaaggc gcgcaactgg tcattatgac gattgcgctg tcactggcga cattcatgca
    13621 ggtgctggac tccaccattg ctaacgtggc gatccccact atcgccggga atctgggctc
    13681 atcgctcagc cagggaacgt gggtaatcac ttctttcggg gtggcgaatg ccatctcgat
    13741 cccgcttacc ggctggctgg caaagcgcgt cggggaagtg aaactgttcc tttggtccac
    13801 catcgccttt gctattgcgt cgtgggcgtg tggtgtctcc agcagcctga atatgctgat
    13861 cttcttccgc gtgattcagg ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct
    13921 attgctgaat aactacccgc cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac
    13981 ggtgattgtc gcgccaattt gcggcccgat cctcggcggt tatatcagcg ataattacca
    14041 ctggggctgg atattcttca tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact
    14101 gcaaactctg cgcggacgtg aaacccgcac cgaacggcgg cggattgatg ccgtggggct
    14161 ggcactgctg gttattggta tcggcagcct gcagattatg cttgaccgcg gtaaagagct
    14221 ggattggttt tcatcacagg aaattatcat ccttaccgtg gtggcggtgg tggctatctg
    14281 cttcctgatt gtctgggagc tgaccgacga taacccgata gtcgatctgt cgttgtttaa
    14341 gtcgcgcaac ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg
    14401 cgctattgtt ctgctgccgc agttgttgca ggaggtctac ggttacacgg cgacctgggc
    14461 aggtttggcc tctgcgccgg tagggattat tccggtgatc ctgtcgccga ttatcggccg
    14521 cttcgcgcat aaactggata tgcggcggct ggtaaccttc agctttatta tgtatgccgt
    14581 ctgcttctac tggcgtgcct atacctttga accaggtatg gattttggcg cgtcggcctg
    14641 gccgcagttt atccaggggt ttgcggtggc ctgcttcttt atgccgctga ccaccattac
    14701 gctgtctggt ttgccaccgg aacgactggc ggcggcatcg agcctctcta actttacgcg
    14761 aacgctggcg gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc
    14821 gatgcaccat gcgcagttga ctgagtcggt aaacccgttc aacccgaatg cccaggcgat
    14881 gtacagtcaa ctggaagggc ttgggatgac gcaacagcag gcatcaggct ggattgccca
    14941 gcagatcacc aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg
    15001 aatattcctc gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg
    15061 cggcggaggc ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca
    15121 attaattgtg aagatagttt actgactaga tgtgcagttc ctgcaacttc tctttcggca
    15181 gtgccagttc ttcgttgctg ttgatgcgta cgtcacgttc cagaatgcta cgcgcaatat
    15241 cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg
    15301 ggatctgatt ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag
    15361 caacacgctg ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca
    15421 ttggcgagat atcgataatc tctacaccct taccgttaag atggttacgc ataaaaccag
    15481 caaacaggtg ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc
    15541 agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca
    15601 cccgaactgc aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca
    15661 tttagccacc tccggtaatt ttttttaaaa ttttctgaac tctttcttcc caggcgagtc
    15721 tgagtatatg aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg
    15781 gccactcacg agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa
    15841 cggttcggta tcagcggttt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc
    15901 tacaaaatcc tcttcgcgca gaatttccag cggctcttca cgcagcagat tacggtaggc
    15961 ttctgcaaac gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg
    16021 ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc agttcatcac acactttctg
    16081 atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa
    16141 cagatcttta cccacctgca gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt
    16201 caggcccggt ttgcgacctt caaggatcac ccggttccag ttaacgcgcg tacaggcaag
    16261 ttcgctactg ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag
    16321 gaatcgcacc tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg
    16381 cacttcaata tattcaatgc cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg
    16441 ggtaacgcgt tttggacgaa tcggcgcgta cagttcgttt tcaatctgca gcacgttgct
    16501 attgatttgc agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg
    16561 cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc
    16621 aagattgctt tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata
    16681 cggcaggtaa tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg
    16741 caggaaagaa gaacaaatcg ccggagatgc accaaacaga taaggaatga cccaaccgaa
    16801 acgatagtaa ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc
    16861 gcccgagata tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg
    16921 cacgccggaa atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag
    16981 cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc
    17041 tgcgatgtag catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt
    17101 ataacgatgc agatcgcgca taaaggtcaa catatgttca atatcaccat ccactggtgt
    17161 aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga
    17221 acctaatgct tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt
    17281 ttcgcgctcc agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc
    17341 cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt
    17401 agcataactg taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc
    17461 ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa
    17521 gattactggc ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac
    17581 cacgggcatc cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc
    17641 tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt
    17701 taaccctcca aggctattac ccattgttgc tgttaaaact aaaacccagg gataactgat
    17761 cccggaaagc aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct
    17821 gagaaaacta ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca
    17881 tccacggcgt ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag
    17941 accacacact gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc
    18001 gcgggtttat ggtatttgac gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat
    18061 cccaggtgcg ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc tacagccatt
    18121 gggcgacgac catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc
    18181 agcagcatac ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg
    18241 gcctgattca gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca
    18301 atcattgcct gaacatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta
    18361 cggtgcgtag gctccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa
    18421 cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt
    18481 gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta
    18541 gaaatgagat atttttgaag gaaatttttg ggagaagatg gtgcatccgg gaggattcga
    18601 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg
    18661 gaaaatttat tttactgctg atattgatat cgctactaaa gccatatcaa gtaagagatg
    18721 gtgcatccgg gaggattact cgccttgcgg ctcgcccttc gggccgttgc taaagcaacg
    18781 ttatcatccc tggtgcctgc ggttaattcc gctacctctt cgataccttc attgctgaag
    18841 atttcggaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc
    18901 gagtactcta tccagctgag ctacggatgc atcgggaaaa tttattttac tgctgatatt
    18961 gataccgcta ctaaagccat atcaagtaag agatggtgca tccgggagga ttcgaacctc
    19021 cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc aaatggcggt
    19081 gaggcgggga ttcgaacccc ggatgcagct tttgaccgca tactccctta gcaggggagc
    19141 gccttcagcc tctcggccac ctcaccacac gcctcttacg agtgcttcga agaacttgtt
    19201 tatcgctcat cgtcgctgcg tggcgcacat attactttct gagacttata agtcaaacaa
    19261 tttttcctac acttttatcg tttgcacact tcacgttcaa ttagtctgta aaaacgtcaa
    19321 aaagagtgtt ttatcaacag aaaaatggag gtctgacaga tagtagtaat gcaaaaaaat
    19381 ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac tatagagtga aggagaaatt
    19441 ttgagggtgc gtctcaccga taaagatgag acgcggaaag attagtaact ggactgctgg
    19501 gatttttcag cctggatacg ctggtagatc tcttcacggt gaacagaaac ttccttcggg
    19561 gcatttacgc caatacgtac ctggttgccc tttaccccta aaactgtcac ggtgacctca
    19621 tccccaatca tgagggtctc accaactcga cgagtcagaa tcagcattct ttgctccttg
    19681 aaagattaaa agagtcgggt ctctctgtat cccggcatta tccatcatat aacgccaaaa
    19741 agtaagcaat gacaaacaca ttacatgtaa gcagtcatgg cattacattc tgttaaacct
    19801 aagtttagcc gatatacaca acttcaacct gactttatcg ttgtcgatag cgttaatgcg
    19861 aatgccgtga agcgagtcca cggcattgcc tgacgcttat attattgcaa tttcgcgctg
    19921 acccagcctt tcacactggc taacgctgca ggtaaggccg cagcatccgt accaccggct
    19981 tgcgccatgt caggacgtcc accacccttg ccgcccacct gctgagcgac cataccaatc
    20041 agttcccctg ctttcacacg atctgtgacg tccttagata cgcctgcaat cagagaaacc
    20101 ttaccttcgg ctaccgttgc cagcacgata attgtcgacc ccagctgatt ttttaaatcg
    20161 tcaaccatgg tacgcaacat tttcggctca acaccgctaa gctcgctaac caacagctta
    20221 acaccattaa catcaattgc cttactggaa agatttgcgc tctcctgtgc ggcagcttgt
    20281 tctttaagct gttgtagttc tttttctaac tgacgcgtac gttccagtac tgagcgcact
    20341 ttatccgcca gattattgct atcgcctttc agcagatgcg cgacttcgct taagcgatcg
    20401 ctgtctgcat gaacggtggt gatagcgcct tctccggtta ccgcttcgat acgacgaacg
    20461 cctgcagcag tacccgattc agagatgatg cggaacagac caatatcacc agtgcggctg
    20521 gcgtgagtac cgccacacag ctcggtggag aaatcgccca tgctcagcac gcgtacacgc
    20581 tcatcatact tctcgccgaa cagcgccatc gcacctttcg ctttcgccgc ttcgagatcc
    20641 atgatgttgg tttcgatcgg caaattgcga cgaatctgtg cgttcaccag gtcttcgacc
    20701 gcacgaatct cttccggttt catcgcttcg ttgtgtgaga agtcgaagcg cagcaccttg
    20761 tcgttaacca gtgaaccttt ctgcgataca tgagtcccca gaacctggcg cagcgcagcg
    20821 tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc ctcatcaaca
    20881 tcagcctgca cagcgtcgcc cactttcaga gaacccgcag caagtttacc gatgtgacca
    20941 atcgcctggc cgtatttctg cgtatcttcc accgcgaagg agaagttagc gcctttcagt
    21001 tcgcctttat caccaacctg accgccggat tccgcataga atggtgtttg atccagcacg
    21061 accacagctt cctggcctgc attgattgca tcaaccgctt taccatcaac aaacagcgcg
    21121 gtcactttgc cgttcagttc cagatggtca tagcctttaa attcagatgc actatcaaca
    21181 cggatcattg cgttgtaatc ggcaccaaag ccgctggctt cgcgcgcgcg acgacgctgc
    21241 tcttccattg ctgcttcaaa accagcttcg tcaactttga tgttgcgctc acgacaaaca
    21301 tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca gacggaaagc cgtttcacca
    21361 tccagcgtat caccagaaag ttttgccagc tcttcatcca gcaacgccag accgcgctcc
    21421 agagtacggg caaactgctc ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg
    21481 cgtttcaggt cttcacccgc agagcccata acgtcgatca gcggaccaac cagtttgtag
    21541 aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca ctgcgcgacg aatgatacga
    21601 cgcagtacat aaccacggtt ttcattggac ggcattacgc catccgcgat caggaacgca
    21661 caagaacgaa tgtggtcagc gattacgcgc agcgatttat tgctcagatc ggtcgcgcca
    21721 gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt cgatgtcata gttagagtta
    21781 acgtgttgca gcaccgcagc aatacgctcc agacccatac cggtatctac agacggcttc
    21841 ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact gcatgaagac gatgttccag
    21901 atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg gaggaccacc ccaaatgtgg
    21961 tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc acccatctgc
    22021 cagaagttgt cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg
    22081 cgcgggatcc cgacttcttt ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg
    22141 gtaacccaca gacgctcttt cggcagggca aaccattttt cgctggtcag cagttcccat
    22201 gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg
    22261 aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca ggtcgttgtg tttaccaccc
    22321 gcacgcacgc agcgttggga agtggtagcg cgggaataat tacgcttgtc gagcccaagg
    22381 aacacatcct tgaactggtt catcccggcg ttggtaaaca acaaagttgg gtcgttatgg
    22441 ggtaccaggg agctgctggc aactacctga tgtcccttac tatggaaaaa gtcgagaaac
    22501 gcctgacgga tctcagcggt gctcttgctc ataattatcc tgaaatcaag ctaacgaaat
    22561 atcgccacca gctccagcgt gtcttaaccg ccgggctggt aactgaaaag tgggaataag
    22621 ataagttttc ttgactggga agtaaaatac cgtatgcgtt cagtcggcaa aatttcgcca
    22681 aatatcctgg atatcttcca tcagatagcc acgatagagc agaaaacgct ggatcttaac
    22741 tttttctgaa aagacagttg gcaaaggttc accatatttt cgcgtcgcct gatcgcgcgc
    22801 cagtgcgcac cagtcgatgt cacattcacg catcgctttt tctgtcgctt cgcgggaaat
    22861 acctttctga ttcagttcct gacgaatacg tgcaggtcca taacctttgc ggctacggct
    22921 ggcgataaag cgcgcaacaa atcggctgtc atcgagatag ccatgttcat ggcaccaggc
    22981 aataacgcgc tcgtaatctt ctgccgtagc atcaatctct tctgggccat ttttgcccat
    23041 aatcggtgcc acgagtttac gtcgcagttc ttgctcactg tgatcgcgca ccgccagaat
    23101 gcgtaccgca cgatccaaca ggcgagcata tgccgggcga cgggacgttg attctgtcat
    23161 ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga tgcgacccag gttttgctga
    23221 taaacagatt taatagtcaa tgagtgtgca gaatgttccc gcaggcccaa ttcgttagag
    23281 gatcgaggtg agagtaattt ttcacgccag tgctggcctg ttcccctccc cctaaccctc
    23341 tccccaaagg ggcgagggga ccgtccgtgc acacattatc tattatcatc aataacgaac
    23401 gattaaaaat cttcgttagt ttctgctacg ccttcgctgt catctacaga gaaatccggc
    23461 gttgagttcg ggttgctcag cagcaactca cgtactttct tctcgatctc tttcgcggtt
    23521 tccgggttat ctttcagcca ggcagttgca ttcgctttac cctgaccgat cttctcacct
    23581 ttgtagctgt accacgcgcc tgctttctcg atcagcttct cttttacgcc caggtcaacc
    23641 agttcgccgt agaagttgat accttcgccg tagaggatct gaaattcagc ctgtttaaac
    23701 ggtgcagcga ttttgttctt caccactttc acgcgggttt cgctacccac cacgttttcg
    23761 ccctctttca ccgcgccgat acgacggatg tcgagacgaa cagaggcgta gaatttcagc
    23821 gcgttaccac cggtagtggt ttccgggtta ccgaacatca caccaatttt catacggatc
    23881 tggttgatga agatcagcag cgtgttggac tgcttcaggt tacccgccag cttacgcatc
    23941 gcctggctca tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc gccttcgatt
    24001 tccgctttcg gcgtcagtgc cgccacggag tcaacgacga taacgtctac tgcgccagaa
    24061 cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg tgtccggctg ggagcacagc
    24121 aggttatcga tatcgacgcc cagtttacgt gcgtagattg ggtccagcgc gtgttcagca
    24181 tcgataaacg cacaggtttt accttcacgc tgcgctgcgg cgatcacctg cagcgtcagc
    24241 gtggttttac cggaagattc cggtccgtag atttcgacga tacggcccat cggcagacca
    24301 cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag agatggtttc cacatccatg
    24361 gaacggtctt cacccaggcg catgatggag cctttaccaa attgtttctc aatctggccc
    24421 agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg
    24481 ccgggtaata ccggatagtc aatatgttct gttgaagcaa ttatactgta tgctcataca
    24541 gtatcaagtg ttttgtagaa attgttgcca caaggtctgc aatgcatacg cagtagcctg
    24601 acgacgcacc gcatcacggt caccgctgaa gcattcccgc cgggtaatgc cttcaccgcg
    24661 ggcagtggca aaagcaaacc agacggtgcc gacaggcttc tcttcactgc cgccatccgg
    24721 cccggcgata ccactaatag acacggcata atcagcacga gccgctttca gtgcacctat
    24781 cgccatttcc accacgacgg gttcactcac cgcgccatgc tgcgccagcg tctcttcgcg
    24841 tacgccgatc atctgcgctt tggcttcgtt actgtaggtg acaaatccgc gttcaaacca
    24901 ggcggagcta ccggcaatat cggtaatcac tttcgctacc caaccaccgg tacaagactc
    24961 ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt cacttaactg
    25021 catcagttca ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc cggacaagat
    25081 agcactttcg tcaggaagat ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa
    25141 agctgattag ccagagggaa gctcacgccc ccctcttgca aatagttact gcacgcgcgc
    25201 cagcgccacg gcttgtccta actgccagac cgccattgcg taatgggtgc tgtggttata
    25261 acgggtaatg gtgtagaagt tcggcagacc gtaccagtac tggtaaccgg taccgacatc
    25321 cagacgcagc aggctggctt gttgatggtt gcccagcggc tgctgtggcg ttaaacctgc
    25381 cgtggcaagc tgcgaaatgc tgtacctggt tttgaagcca tttggcaagc ctggagcctg
    25441 accgtttgcc attaccgcga cctgatcgcc tttcacccag ccgtgcgctt tgaaatagtt
    25501 cgccacgcta ccgatcgcat caaccggatc ccacaggttg atatgcccgt cgccgctgaa
    25561 atctaccgca tattgtttgt aagacgacgg cataaactgt acgtagccca tcgccccggc
    25621 aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg cgcgccatca acaggaaggt
    25681 ttccagctcg ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag
    25741 cgcatcgagg atgcgagttt tccccatcac gcgcccccag cgggtttcaa cgccgataat
    25801 cccgacgata atttccggcg gtacgccata cacctgccac gcgcgattca acgcatcttc
    25861 atactgattc cagaaaacca caccgttctg cacgttgtcc ggcgtaataa attttttgcg
    25921 ataacggagc catgcgccgt tcggacctga tggcggtttc accgatgtgg ttggtgcctg
    25981 gttatccatc agccgcagta ccgaatccag acgcttcgcc tgggagagaa tttcctgcaa
    26041 ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg atgaactgct gggcattcgg
    26101 gttattagcg aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc
    26161 gccagacggc gttccggtgg tcgtttcagt ctcagtaggt tttggcttgc tgctacaggc
    26221 ggcaagcaac acaaaaaggg gaagcaatgt tacataacga cgcttgaaca tgaggggtcc
    26281 atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc acgccccaca caaggaagcg
    26341 gtagtcactg cccgatacgg actttacata actcaactca ttcccctcgc tatcctttta
    26401 ctcaaacttt caaattaaaa tatttatctt tcattttgag atcaaaataa cacttttaaa
    26461 tctttcaatc tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag
    26521 agaacaatga tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag
    26581 ggcggagagg tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg
    26641 gttatcatga acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa
    26701 cgttgcgccg gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc
    26761 ttttgcaatc cgatgacctt aagcctgggt cgctttatgc cggaaaagta caaacccagc
    26821 tactacgcgg cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac
    26881 cctggcgaac tgtttgttta tcttggcatt gccagcggtc tgacaacgct gaacctgcca
    26941 cttggcccac tggcggtgag ttatctgctg gttggtctgg tcaccaattt cttccgcggc
    27001 tgggtgaccg atctgaccac cgccattttt gagaaaaaga tgggcattca acttgaacaa
    27061 aaagttcacc tggcaggagc aacatcatga cgcatattcg gatcgaaaaa ggaacgggtg
    27121 gctggggcgg cccgcttgag ctgaaagcca cgccgggaaa aaaaatcgtc tatatcaccg
    27181 ccggtacccg gcctgcgatt gttgacaaac tggcacagct tactggctgg caggctattg
    27241 acggatttag agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta atcgactgtg
    27301 gcggcacatt acgctgcggc atctatccga aacgacgtat tcccaccatt aatatccact
    27361 cgacgggcaa gtccggtccg ctggcgcagt acattgtgga agatatttat gtctctggcg
    27421 taaaagaaga aaacatcact gtagtgggtg atgcgacacc acaaccctct tccgtgggcc
    27481 gtgactatga caccagtaag aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg
    27541 gaatgggcat ggggtccgcc gttgcggtgc tgtttcaatc tggtcgtgac accatcgaca
    27601 ctgtattaaa aaccattctg ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta
    27661 tggcttctgg ccttggtgac tggattgccc acggtcttgc tccgctggcg agccatccac
    27721 tgggtctggt catgctggcg ctcatctgct ccttcccact gctttcacct ttcctcggcc
    27781 caggcgcagt tatcgcacag gttatcggcg tattgattgg cgtgcagatt ggtctcggca
    27841 atattccgcc gcatctggct ttaccggcac tgtttgccat caacgcgcag gcggcctgcg
    27901 acttcatccc ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg
    27961 tcccttctgt actggtgagc cgctttttaa ccggcgcacc gactgtactg atcgcctggt
    28021 ttgtctccgg ttttatctat caatagaggc tgaaacatga ccgttattta tcagaccacc
    28081 atcacccgta tcggcgcgag tgccactgac gccctcagcg accagatgct catcaccttt
    28141 cgtgaaggcg cgccagcgga cctcgaagag tattgcttca ttcattgcca cggcgagttg
    28201 aaaggtgcac tccatcccgg tttgcaattt acgctcgggc agcatcgcta tccggtgacc
    28261 gctgttggca gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc
    28321 gatggtttaa acgaagcgga atttccgggc actgtccatg tggcaggccc tgtacccgac
    28381 gatatcgcgc cgggatcggt tttgaagttt gaatctgtta aggagtaaaa aatgaatcag
    28441 gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct
    28501 gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc aaatgtggca
    28561 caagaaatta acgccgaata tggtgaaagt atggcgtacg gttttggtgc tgacgccact
    28621 agcgagcaaa gcgttctggc gctctctcgt ggggtagatg aaatctttgg tcgcgtggat
    28681 ttgctggtct atagcgccgg aatagcaaaa gcagccttta tcagcgactt ccagctcggc
    28741 gattttgacc gttcgctaca agtgaatctg gtgggttatt tcctgtgtgc ccgtgaattt
    28801 tcgcgtttga tgatccgcga cgggattcag ggtcgcatta ttcagatcaa ctcgaaatcc
    28861 ggcaaagtgg gcagcaaaca caactctggc tacagcgcag cgaaatttgg tggcgtcggg
    28921 ctgactcaat cactggcgct ggatctggcg gagtacggca ttacggtgca ttcactgatg
    28981 ctcggtaacc tgctgaaatc gccgatgttc cagtcactgt tgccacaata cgcgaccaag
    29041 ctgggtatca aaccggatca agtcgagcag tattacatcg acaaagtacc gctcaaacgc
    29101 ggctgcgatt atcaagatgt gctgaatatg ctgctgttct acgccagtcc taaggcgtcg
    29161 tactgcaccg gacagtcgat caatgtcacc ggcggtcagg tgatgttctg atcaacagcg
    29221 gagatccatt aaggatctcc gtgagactat agaatgcctg atgcgctacg ctcatcaggc
    29281 atacaggact tccgccacta cattaaggaa aagttatggt atccgcactc atcaccgtcg
    29341 ccgttatcgc ctggtgtgcg caactggcct taggcggctg gcaaatttct cgttttaacc
    29401 gtgccttcga cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct
    29461 ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca gcagcgcatc gtcgacacct
    29521 tgtttatgaa aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta
    29581 tgcatgcggg tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg
    29641 ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg
    29701 cgaagttatc attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc
    29761 aggccgccat tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc
    29821 aatactttga caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg
    29881 gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta
    29941 tcgatcataa aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta
    30001 gttttatcca tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg
    30061 ttcccctgct ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca
    30121 atgcgctatc cgaactggat aacgaacaaa ctatcctgat gccaggcgga acgtttcgta
    30181 aaaaatcggc ctcatttcac ggacagctgg cagagaatgc cttcgaacat ttcagtttcg
    30241 ataaattgtt tatgggcacc gacggcatcg atctcaatgc gggcgtaacc accttcaacg
    30301 aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg
    30361 actcatcaaa gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata
    30421 agctgattac cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga
    30481 tcgatgtgat cataaccgga gagagcaatg agtgaagcac tactgaacgc gggacgtcag
    30541 acgttaatgc tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt
    30601 gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc
    30661 aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg cacaccggct
    30721 ttttttgtcc atccggcaga agcactgcac ggcgatctgg ggatgattga aagccgcgat
    30781 gtgatgctgt ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt
    30841 ctggaagata aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc
    30901 ctggcggcaa aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt
    30961 gcgccgacct ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc
    31021 atgcaggcgc gcggatttaa tgaagaagat tttgcccgct cccacccagc cggggcactg
    31081 ggcgctcgct tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag
    31141 gtggcgttaa ccgccagcgt gatggatgcg atgctggaac tcagccgcac cggtctgggg
    31201 ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta
    31261 cgtcgctggc tggttggcgg cggcgcactc accacgccag ttaatgaagc gatgacgaca
    31321 ggcggcacca cattacaggc acaaagtcgc gccatcgacg ccaaagagat cctgatgaag
    31381 cgcaaaatca ctgccgcacc ggtggtggat gaaaacggca aactcaccgg cgcaattaac
    31441 ctgcaggatt tctatcaggc cgggattatt taatccttca atcccagacg tttcgccagc
    31501 cgatgcaggt tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttgtgatga
    31561 ttttgtgcca gtgcctggcg aatggtttca cgctggaacg cttctgtcgc ttcacgcagg
    31621 ttttgcttaa caacgggcac cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa
    31681 gcaaaatgtt gtgcctcaag aatcacttca tcgccgctgc gagtggctct cgccagcact
    31741 accgcccgat gaatagcatg ttccagttcg cgcacgttcc ccggaaaacg atagtgttgc
    31801 agtaaatttc gcgctccggc acttaatacc acgcgggaga gccccagccg caaacgacac
    31861 tgctcgcaga aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc
    31921 accgaaagtg gaaacacgct caggcgatga aacaaatcgg cgcggaatcg ccctgccagc
    31981 acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaacta
    32041 cggtcatcgc caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc
    32101 aatgccaacg acaactcgcc gatctcatcg agaaacagag tgccgttatc cgccatttca
    32161 aacttcccgc tgcggttact gatagcgcca gtaaacgctc ctttcacatg cccgaacaac
    32221 tcactttccg ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc
    32281 gcccgtggcg aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca
    32341 ccgctgatca gaacgttgag atcggacgcc gccacaatct caatctcttt tttcaattgc
    32401 gtcatgcctg gcgacaagcc aatcatctgc gtctgtttca ccgcttcaaa cggcgcggca
    32461 tcgcccggca gcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct
    32521 cccgccgcca gcgcggcaat cagccgtaac tcttcgtcgc tgaaaacatc gaactgatcg
    32581 ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt gcccggcaaa caatggcaga
    32641 ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc atagggatcg
    32701 ggcaattcgc tgtctgcagg aaagcgcacc acgtccccgg cgcgggcaat cgcttcaagc
    32761 cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat cctttgccag accgtcgatg
    32821 gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc
    32881 acctgacgta gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc
    32941 aattcgatgg cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca
    33001 atttgactat agatattgtc atatcgacca tttgattgat agtcattttg actactcatt
    33061 aatgagcata attttattta tagagtaaaa acaatcagat aaaaaactgg cacgcaatct
    33121 gcaattagca agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg
    33181 tggtgaaaaa taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg
    33241 gcacggaata taaaacgctg cgcggcagca gttacaacag ctacctcatc cgcgaagaaa
    33301 aaaacgtgct gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc
    33361 gtaatgaaat cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc
    33421 acgctggggc gctgaccgaa ctgatggcgc aaattcccga tacgccgatc tactgtactg
    33481 ccaacgctat cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg
    33541 tgaaaactgg cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacac
    33601 caatgctgca ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca
    33661 gtaacgatgc tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc
    33721 agacggagct tttcgagcag tgccagcgtt actacgccaa tatcctgacg ccgttcagcc
    33781 gcctggtaac gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag
    33841 cgacctccca cggcgtggtc tggcgcgata acccgacgca aattgtcgag ctgtacctga
    33901 agtgggccgc tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata
    33961 acacccgcat gatggctgac gctatcgccc agggaattgc ggaaaccgac ccacgcgtgg
    34021 cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc
    34081 gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg
    34141 ccgggctggt ggaggagatg actggtttac gcttccgtaa caaacgcgcc agtgctttcg
    34201 gctctcacgg ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg
    34261 gtttcgaaat gtcgcttagc ctgaaagcca aatggcgacc agaccaggac gctctggagt
    34321 tatgtcgtga acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga
    34381 gcacggtgaa tacggtggtt aaagaagaaa cctctgccac cacgacggct gacctcggcc
    34441 cacggatgca gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc
    34501 aggacgttgc gccaggaacg ccgtggagtg aagttccgga taacttcctc tgcccggaat
    34561 gctccctcgg caaagacgtc tttgaagaac tggcatcgga ggcaaaatga gtaacggcat
    34621 tgtgatcatt ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga
    34681 cgccactatt ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaacctga
    34741 cctcagccat gttatcagtc aggggcaacg tgccgacgac cttacccgcc agacggcggg
    34801 tgaatttgcc gagcagttta atctgcgcct gtttccgcac acctgggtga cggatatcga
    34861 tgccgaagcc cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctagtgct
    34921 ggcaaccggt gccagcgcct ttgtcccgcc agtgcctggg cgtgagttaa tgctgacgtt
    34981 aaatagtcag caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt
    35041 gattgtcggt ggtggcttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa
    35101 agcggtcacg ctaatcgaca acgctgccag tattctggcg tcattaatgc caccggaagt
    35161 aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt catctgttgt tgaaatctca
    35221 gttgcagggg ctggaaaaaa cagattctgg cattctggca acgctggacc gccagcgcag
    35281 tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg
    35341 acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa
    35401 tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct
    35461 ccagccgatt caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc
    35521 actgaaacta ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc
    35581 aggcgaaacc cagcgtcagg atttacgctg gcagattaat accgaacgcc agggaatggt
    35641 ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg aggatcggat
    35701 gaaagaggca tttggattgt tgaaaacgtt gccgatgtag gtgggctact gtgcctaaaa
    35761 tgtcggatgc gacgctggcg cgtcttatcc aacctacggg gagcgcatgt gttggtcaca
    35821 tactgcgttc atgtcgcatc cacctactgt gcccaaaatg tcggatgcga cgctggcgcg
    35881 tcttatccga cctacgggga cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg
    35941 caatggtgtc caaatgcaac acgttttatc cgttctggac ttcacccgct aaccaacgcg
    36001 ccgcagcaat aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg
    36061 gaaagagcaa tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat
    36121 gaataacccc gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca
    36181 tcaacgcggc aaaaccctgc gccagcgcat catgaaacgc ccacgcgcgt tgattaaccg
    36241 gtgcctgcca gttcagccac tgctgccaga aagtggcgag atccagttga ttgtccacca
    36301 gcggcattgt caccggatgc gtcactccgt ggcacgaagc tgcgagtgcc tccagagcac
    36361 aagccgcttc accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccacgg
    36421 catcgaacaa ccgcccacac gatgacgcca gcggcgcgtt aattccacgc tcaatggccc
    36481 gcgccagcac gctccagttt tgctgttgca cacttgctgt ttcagaataa ttctgccact
    36541 ccggcacaaa gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc gccgccaaat
    36601 cgccacccgg aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca
    36661 cccgcagaca ctcgccgccc cacaaagcgc cgttctcccc cataccgata ccatcgagcg
    36721 tcaaagcaat gacatccccg ccatccagcg gccactgatg ctctgccaga cacgccgctg
    36781 cgtgggcatg atgatgcagc accgtttgcg tcggcatatt catttcgctc gcccactggc
    36841 tggagacata gcccggatgc acgtcatgca caacgtattg cggagtgaaa tcgtagatgt
    36901 tttgcatcag gcgtaacgct tcgcgccact gcatctggat gccatcgtca cttaaatcgc
    36961 ccagatgctg actcaacacc gcttgttcac cgcgcaccag gcagaaggtg tttttcagat
    37021 ccgcgccgag acacagcaca ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat
    37081 ccggcacata cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat
    37141 ccatccgctg cacgatgtcg cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat
    37201 ccgccagcgc ctgttcgttg ctgatagctg gtggtttacc gctcaggttg ccggaggtca
    37261 tcaccagcgg gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca
    37321 ttaccccgac ttcgttaagg tcaggggcga tatcatcaca aagctcagga acgtattttt
    37381 tatccaccag cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta
    37441 aaccgtcagc cactggcaac atgactgcca gcggtttcgc cgggcggtgt ttgcgctccc
    37501 gcagtgtcgc caccgcgtta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc
    37561 ctttgatggc gacaattttg cccattttta actgtgcgat agctgcctgt aatgccgcct
    37621 cttgttccgc atgttcacca tgacttaccc attcaagatg cgggccacac tccgggcagg
    37681 ccaccggctg ggcgtggaag cgacgatcga gcgggtcgcg gtactcttta tcacaggctg
    37741 gacacagcgg aaactccgcc attacggtaa acgggcggtc gtaaggcatg gcgcgaataa
    37801 tggtgaaacg cgggccgcag tgggtacagt tgataaacgg ataacgataa cgccgttcgc
    37861 ctggggtatt catttcggca aggcaagcag ggcaagtagc ggcatccggg acaatttgcg
    37921 tattcatggc acctcccgcg ctctggcgga tagtgaactc ggtgggcagt tgtgaccaga
    37981 taaacggctc acgctcgacg ctatcaatac gcgccagtgg cggacagtgc tgatgcaatt
    38041 gaacaagaaa cgtttccggg tcttccagca gccggacttc tacgccatcg ccgtcattac
    38101 agacatcgcc gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga
    38161 cgccctgcac tttgccacga atacgcagtt ggacaccgca agatgtgttt tttgccattg
    38221 agttattccc gccatcatga attgcgtaac ccgtccagcc ggacacgaca gcgtcgcatc
    38281 cggcagtcac aggtcggcga taccgcccgc ttcgtattct acgaatattt ccgggaattc
    38341 ctttgatgcc agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt
    38401 ttctgcgctc agttgttcaa gtttattacg atcgacacaa attagcgcat gggtcgggca
    38461 agccgccata cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc
    38521 agctttgtca gcccgtacat tcagccccac gccgctgttg cgaatcaccg gacgtaccac
    38581 cacttccatc gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa tacaacgttc
    38641 ctgcatcaca tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc
    38701 gcacggtgca tcttcacact gacggcaaac tgtcgccgtg gaaatgttca cacctttaat
    38761 gacatggata cgcggtaaaa atgtttccgg ggtcagcgat gcacagtcct gattttcctg
    38821 atgagaaacc acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc
    38881 aatgatgaaa cggttcatca aattctccag caatgacagt taatgcgccg atacattcac
    38941 aaatcatgcc agtttttaat ttactgttat ttaaggaaat taatttctgt aatgcaggaa
    39001 aaacgatgtc atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaaggg
    39061 caatgagtga gtcgcggcag atcaattttc cgctgaaggt tttcggcggt gagaaatccc
    39121 cgccatcgag cataaaaatc agccgtccaa taatttcctg aatcatctca gtcaccggaa
    39181 tttttacgct ggagagcgcc ggaacgatat agggggcaat tgcgatatca tcgaatccga
    39241 taactgacac ctgctctggc accgctacgc cgcgctcgtg caacgctttc atcgcaccta
    39301 tcgccatatc gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaaca
    39361 tttctacccc ttcggccccg ctggcaggcg tccatctacc gttagcgata agtttttcat
    39421 tgagcgcaat accgtgctgc gccagcgcgt ctttatatcc ggcaagacgt tcaacgctgg
    39481 tgggagaatc catcgaaccg gtaaggaaag caatctcctg atgtccggcg tttatcaact
    39541 ctgccacggc gttaaaactg gtctgtttat gatcgcacca gacgctatgg ctgctgtttt
    39601 tgcgcaggcg gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcga
    39661 tctcatccac gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca
    39721 gatactgaat cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact
    39781 gccgcccttt ctcttccgcc attcgcgcgg catgaaacag taattcacta aaataaatgc
    39841 cgtggtaaag cgtgttggtc actaccagcc ccagcgtctg agtactcttc gccgacaaat
    39901 tgcgcgccag caagtttggg cggtaaccgc tctcttctac cgcctgaaac acgcgatctt
    39961 tagtctcctg gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa
    40021 ccccggcgcg cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa
    40081 tcaacgcagt gtactgcacc gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt
    40141 caggtgaccg gtttcacaaa tataaaaaat gaacaattaa ctctcttgct tatttagtga
    40201 caactattca tgattttgtg aaaccggttt cctaattccg tttcagcatc ggcatttttc
    40261 cgttacgtcg actgataaca actacatcta ccctactgat aacaggataa aatccgatgg
    40321 ccaaaaatta tgcggcgctg gcacgctcgg tgatagcggc actgggcggc gttgataaca
    40381 tctcggcggt cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat gaccagctta
    40441 tcgacagccc gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc
    40501 agtgtcaggt gattatcggc aataccgttt cacaagcctt tcaggaagtc gtcagcctac
    40561 tgccgggaga tatgcagccc gcactgcccg tgggtaaacc caaactcacg ctgcgtcgca
    40621 ttggtgcagg gatcctcgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta
    40681 tcggcggatc gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa
    40741 aaggatcgcc gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc
    40801 cgctgatggt cgcggcatct gccgccatca aatttaaaac caatatgtcg ctggcgattg
    40861 ccatcgcggg tgtgctggta catccgagtt ttattgaact gatggcgaaa gcggcccagg
    40921 gtgaacatgt cgaatttgcc ctgattccgg tcaccgcggt gaaatacacc tacacagtga
    40981 tcccggcgct ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta
    41041 caccggcggt gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc
    41101 tggcaatcct gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg
    41161 tttacaccat tcatggttat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc
    41221 ctctgctggt aatgaccggg atgcaccgcg tctttacgcc aaccatcatt cagaccattg
    41281 ccgaaaccgg caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg
    41341 gcggttcatc actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc
    41401 tggctgcggc ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg
    41461 cgatccgcct gaaacgtccg cttatcgcca gtcttatcag cggctttatt tgcggcgcgg
    41521 ttgccggtat ggcggggctt gccagccact caatggcagc gccggggcta tttaccagcg
    41581 tgcagttctt cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc
    41641 tggcggtcat gctgtcgttt atcctcacac tgttgctcgg ctttgaggat attcctgttg
    41701 aggaagcgac tgccgaggcg cgaaagcatc agagcgtaca accgaccgtc gccaaagaag
    41761 taagtcttaa ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc
    41821 gcttgccgcc aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaagggc tgaccactgt
    41881 cgatatgatc ccacacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt
    41941 tcagttgcgc gatgacgagt tttatcccag ccatgaggcg acggattttt atcatcgtta
    42001 taaagaagat atcgccctga tggcagagat gggattcaag gttttccgta cctcaattgc
    42061 ctggagccgt ctctttccgc agggcgatga aatcacgccc aatcagcagg gcattgcttt
    42121 ttatcgttct gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg
    42181 ccacttcgat gtgccgatgc atctggtcac cgaatatggc tcctggcgta accgcaagct
    42241 ggtggagttt ttcagccgct acgcccggac ctgctttgaa gcatttgatg gtctggtgaa
    42301 atactggctt accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg
    42361 tctggtgttt gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca
    42421 gctggttgcc agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt
    42481 gggctgtatg ctggcgggcg gtaacttcta cccttacagt tgcaagccgg aagatgtctg
    42541 ggcggcgctg gagaaagacc gggaaaacct gttttttatc gatgtgcagg cgcggggcgc
    42601 gtatccggct tactctgccc gcgtattccg cgaaaaaggg gtaaccatcg acaaagcacc
    42661 gggcgatgat gaaatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc
    42721 gcgctgcgcc tcggcggaga tgaacgccaa caatagcagt gcggcgaacg tagtgaaatc
    42781 gctgcgtaac ccgtatctgc aagtgagcga ctggggctgg ggtattgatc cactcggtct
    42841 gcgtatcacc atgaacatga tgtacgaccg ttatcagaag ccgctgtttc tggtggaaaa
    42901 cggcctcggc gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat
    42961 cagctattta cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct
    43021 gatgggctac accacatggg gctgtattga tttagtttcc gcctctacgg gtgaaatgag
    43081 caaacgctac ggttttgtct acgtagaccg tgacgacgca ggcaacggca cgctgacgcg
    43141 cacacgtaag aaatcgttct ggtggtataa aaaagtgatt gccagtaatg gtgaagattt
    43201 agagtaagca acagtgccgg atgcggcgtg aacgccttat ccggcctaca tgtcgcacga
    43261 accaggaagg cccgataagc gcagcgcatc gggcaatctg gggtttggca tcagtcaaaa
    43321 tcaaagaaac cattccggct taacgccaaa tcgggctgaa agtgccttaa tgtgggatat
    43381 cgtcagggat cgttgtccag acaaaatctg actgaccagg gatttagagc cgatttcgtt
    43441 cttcaaatcc gcataggtca gattatgttg atcaatcagc gtgcgaagta acgccacgcc
    43501 aacaggcatt gccgcaacgg ctttgtcgaa ctctgcaaac ttttcgttgt tgttttcata
    43561 ctctgcaatg cggctcgcca gaaagtcgat aagtggattg gtgtcatctt cttcaatcaa
    43621 atattcgacc agttccagtg cttcacgata atcatcttct gagtcactgc ctcccagaaa
    43681 ggggactgca ttgaccagtt ctcttgttgc tttaacagcg cgtgcagcat tagcagtcat
    43741 tctttgttct ctctgtagta acgtgtcagt ttgtcatact ctgcgtgatt cgttatgtgc
    43801 ttcacaaaga accgcttatt aacaaagttt atgtaggcaa taaccctaag attgttcccg
    43861 ccgacatcca gaacgtacca tttgttgcga tatctaaaat tgtcgaggtt gggaaacgcc
    43921 gttcgcattt cttctggtgt agaaaaatcc gtttccttaa taacccgata caatgcctga
    43981 tgagccagag cgtcgttagg atatttacgc gcagactctt caaatggcgc tttagaaatg
    44041 atgtgcattt ttaagtttta acttccttat agtaagtaaa cagccacatc tgcgatgttc
    44101 tatccttatg tgttcggaaa ctatagctca tcaaatccaa gttcacaata tgtaaacact
    44161 tgtgcgcgat ataattaccc ccaaaatcct catagccact ctgcaaaaag tcagttctgg
    44221 ttcacccctc aagcacatcc ccctttatca agcaacgctg cgtctgcgcc agatcttccg
    44281 tttgcgggtc gcgcccggcg atgggtttga ggacgagttt acggttgggg gtataagtaa
    44341 aacgcgtgag cgcgggatag cttaacttgc caaaccagcc gtgaacacgc tgccatttgg
    44401 cgtcttttac ctgatacaga ctggttatcc agtaaccaga attaaactgc atatccagca
    44461 attgcgtgtg accattgcct tgcaaatcca gcaaatcatc tacgccagtt ttgcttgcgg
    44521 tgtagaatcc cctgggttca aagacacagg gacggccttc gcgcgtgaac gtcataagaa
    44581 ttaaatgtgc ataaggagcc agaccattac cggtacttgg taaccagata accagatcct
    44641 gaatgccgtt acgatcaaga tcggcgcgat agagttgccc tttgcccgct atgtttgcca
    44701 gcatacccac aggcacagtc caggatttac cggcgcggtc tttagcggtt aattgccagt
    44761 aatcttcttc atttggcatc ttctcaagcg tcacgccagc aacgcccact ttcctaaaat
    44821 gccaggtttg ctcggcatca taaggcatgg tcaggagagg tttcagcgcc cgcttttgcg
    44881 cgtattcgct gtcactcatt ggcagatatt cggtaaagct ggtggcctgt gcaggcagca
    44941 ctaagctcaa taaaaaagcg cacatccctg ttctgatgtt caattccctg aattccctga
    45001 tggagaatta ttcttcttcc accgctaact gcgcaaatcc accatttcct tcccagcctt
    45061 ccagtcgttg ataaacggtt tctaccgcat ctttaatcgg ctgggtcatc gggtagtaaa
    45121 agccgacgat atccggctga atgccgagga aaatcacttc accaatgtct tcttttaact
    45181 ggtcgataag gtaattcaac ggcatgttat gggtagtcat cataaacatc tcggcgatat
    45241 catccgggtc gatgatgcgg atttcgccgg ggtttagccc catatccgtg gcgtcgacaa
    45301 tcagcagtcg tgtcgggcgc agttcacgga tggcgacgat gtcgttttcc ggtgcactac
    45361 cgccgtcaat caccacccag ttacctttcg gcgcggcggc gcacttttcc gccagcagcg
    45421 ggccagcgcc atcatcgccc atcatgctat tgccaacaca gagtaaaacg tcagtcacgc
    45481 agtctcctca ccatcaaata gatggcgttt tcctgatgaa tatcatgcag catacttaac
    45541 agcattgtac tccattcctt ctgctgcgaa gtttgcgctg cccgggcttt atcgaatgcg
    45601 ttggccagca tcggcacatg gttaatgtcg atgacgatct cgccatattt cggtacgcct
    45661 tccattttgc ggcgggcttc gctgcccgcc tccagagtgg caatccatgc cagatattca
    45721 tcccatgggc aggtgagcgc cgcttccagg caatcgataa ccccaaggtg gtgaccaatc
    45781 gccaggctgt aatagaccac ctgctgcgcc tcggcgggcg tggcatcgtt ctcatcaata
    45841 aatttacggc tcagttgact gaacaccacc ttttcactca tcggatacgc gcctcttcaa
    45901 caacatgatt cagatggctg acaatctcgt tcagacgcgg atcgttttcc gcttccagcc
    45961 agcgtgcaac ctgttcttcg ccctgcccta actgtgtaag gtaatcatcg gcaatctgac
    46021 gaccgtaacg ataacccgcc aggcgacgtg cttcgcgatc cactttcacg cgcagcggct
    46081 gcaccatatc gccatgcaga atctcagccg gttgttcatc ctgctcaccc gggccacggg
    46141 cgtgaatttt ctgctccagc aggccgagtg ccattgcaaa gccgtacagc gtggcggcag
    46201 gcgttggcgg gcaacccgga atgtagacat ctaccgggac gattttatcc gtaccgcccc
    46261 acacgcagta gagatcgtgg aaaataccac cgctgttacc gcaggcaccg taggagatac
    46321 aaattttcgg gtccggcgcg gactgccacg cacgcagcgc aggggatcgc attgcacggg
    46381 tgaccgcgcc ggtaaacagt aaaatatccg catgacgcgg tgaaggaacg actttaatgc
    46441 cgaagcgttc tgcatcaaac agcggcgata aagtggcgaa aatttcgatt tcgcagccgt
    46501 tgcagccgcc gcagtccacg cggtaaacat aggcagaacg tttgattttt ttcagtaacg
    46561 acgccttcat gctggcgatg gattcatcca ccgtcatcgg gaccggaatg ccgttggcgt
    46621 cacgggggcc taataaattg ctcatcagat ggcctctttc atatggcgag tcagttcaat
    46681 acggtcggac ggcaccaggc atttctggcg cttacattcc gggcaagtct caaagctttc
    46741 gcggtggttt tccgcgcggc tgtcaccatt gtgcttaagc agcgcaatgg cgtagtcgat
    46801 ctctttctgg acggcgaaag gacgattgca gacgcgacag ctgcacagca caaaacggga
    46861 ctgttgcagg aaatcttctt tcttccacac cgccagttcg tactcttgcg acagtttgat
    46921 cgctgccgtc gggcagactt cttcgcagcg tccacagaag atgcagcgcc cgagattaaa
    46981 ctcccacgcc agctcaccgg tggcgaggtc agtttcaacc gttaaggcgt ttgacgggca
    47041 ggcattgacg caggccgcac agccgatgca ctgctgcggg ttctgctccg gcttaccacg
    47101 gaagttttta tcaaccgcaa tcggctccag cggataagac gaggtcgccg tgccggtttt
    47161 gatgactttt ttgataaagg taaacatggc gattccttat ttcagcggcg agtttttacg
    47221 ctcaatgctg taacgctcga gttctttgta cggcaccact ttgctctttt tcttacgcac
    47281 atcgaccacg gtcatgcggt cggtacagga gtagcaaggg tccaggctac cgataatcag
    47341 cggcgcatcg gaaacggtgt taccgcgcag catgtagcgc agggtcggcc agttcgcgta
    47401 ggtcgcggca cggcagcgcc agcggtacag cttctggttg tcgccggtca tgctccagtg
    47461 gatatcgtcg ccgcgcggcg cttcggcaaa gcccagcgcg aaacggtgcg gaatgtaggt
    47521 aaagccttcc accatcagcg gaccgcccgg caggttatcc agaccgtagt cgatcatgtt
    47581 cagcgcggta taaacttcgt tgatacgcac tttcagacgg gaaataacgt cgcagccctg
    47641 ctcgctgtgg acttccattg gcagcaggcc ataaccgaca aacgggtgat cggcgcgagt
    47701 atcgcgggcg tgaccgctgg cgcgaaccat cgggccaacg ttactgaaat cgcgggcgat
    47761 ttccgggtcc agacgaccaa tgccgacggt gcgctgttcc atgttcggcg tactcagcag
    47821 cacatccacc agctcctgca cttcacgacg catctgttgc gccagctggc gggtctggat
    47881 catgtcatct ttcaacagat cgcgacgaat cccgccgatc aggttcaggc cgtaggtttt
    47941 acgcgcaccg gtgaggatct ctgccatttt catggaggtt tcacgcacgc ggaagaactg
    48001 cataaagccg gagtcaaagc cggtaaagtg gcaggcgaga ccgaggttga gcagatgcga
    48061 gtgcaggcgt tccacctcca gcagaatggc gcggatcatc tgcgcacgtt ctggcacctg
    48121 aatacccatc gcgttttcta ccgacgtggt gtaggcggtg ctgtgggcaa agccgcagat
    48181 cccgcacaca cggtcagaga ggaaggtcac ttcgttgtaa cccatacggg tttccgccag
    48241 tttttccatg ccgcgatgaa cgtagaacag acggtagtcg gcgtcgataa tgttttcgcc
    48301 atcgacgaac agacggaagt ggcccggttc gtcagaagtg acgtgcagcg gaccaatcgg
    48361 cacgacgttg tttttcttgt cgcccagttc gttgatgaac tcgtaggttt cagcatcggt
    48421 agtcggtgcc ggacgctgac gataatccat gctgtcttta cgcagcggat aaagttcatc
    48481 cggccagtca tccggcagca ccagacgacg ttcatccggc agaccaaccg gaatcaaacc
    48541 gtacatatcg cgcacttcgc gctcgcccca caccgccgcc ggaacgcgcg gcgtcacgga
    48601 cggatactcc ggtttgttgg cgtcaacttc gacgcgaacc gttacccaac acttggtgcc
    48661 cttctccatc gacagcacgt aataaacggc gtaatgacca ttcagtttgc gttcgtcgtt
    48721 accaaacagc accgacagcc agccaccctg cttgtagtag agaaactcca ccacttccgg
    48781 caggtagttc accttcacgg tcacggtcag ctgatctttg gtctgccagg cgtggtccag
    48841 cacgacaccc ggaaatgcct cattcagcgc ggcgagataa tgttgaccta atttttcttc
    48901 agacatgctc aaactctctt taatcacgcc gccagcaagg agacgaacgc taaaaatgca
    48961 aagccaaacc cagcccaggt aatgcgcgga gtaatatcaa gacgcagacg ggccatgctg
    49021 ttttcgaaca gcgcgataac caggacgccg acgaccagtt tgacgatggc aatcaccagc
    49081 gccagcagca gcccaccggc ggtgaaggtt tccatctgtc cccacgggat aaatacgccg
    49141 acgaacatct gcagcaccac cagctgtttc aggctgatac cccatttcat gacgccaaag
    49201 ccgctgccgc tgtattcaga gagcgggcct tcctgcagct cctgctcggc ttccgccagg
    49261 tcgaacggca gtttgcccat ttcgataaag gtggcgaacg cacaggcaca aagcgccagt
    49321 accagcggga tgctctggct cagcggccag tgataaacgg tgtcggtgat gttgctgatg
    49381 ttggtggaac cggcaacctg tgcggcaacc cacagaccaa gcagcagcat cggttcgacc
    49441 agcacgccaa gcatcgcttc acggctcgcg ccgatagcgg taaacgggct accggtatcc
    49501 agaccagaaa tggcaaagaa gaaacgcgcg atggcaaaga gatacagtaa ggtgattaaa
    49561 tcacccagtt gcggcagcgg agaaccgacg gtcaccaccg gcagcgcagt agcgatagtc
    49621 agcatgacgc ccaccatcac atacggcgtc aggcggaaca cccagccgga ggcatccggg
    49681 ccaacgctct ggcggcccag cagtttgata atgtcacgat actcctgcaa cacacccggc
    49741 ccacgacggt tatgtaagcg ggctcgcgcc acgcgggtaa taccggagag cagcggcgca
    49801 acggcaaata gcaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    49861 gtgaaacaat aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca
    49921 acgcactccc ctcgcactgc cagcccggca ccagagacac cggattcagc catttgcgta
    49981 gtttcagcac cggcgcaaac gcctgtttca ccggcatggc aaaaccgtga gcggtaatca
    50041 ccattgattt ttcgtggtcg tagccgcaca cccaggccgc gccgcgggaa cgcgatggca
    50101 aacgatcgcc tttgcaaatc gccataatga tgaatggcag cagcggacag gcaatcagca
    50161 gcaatgtgat cattggttga gaaacggtgg tgttagcagg ctccagcggc agaggtacag
    50221 cagcagacag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    50281 ccagtgccac tacgcttacc ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt
    50341 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    50401 gcgcaccggt aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg
    50461 cgccgctatt gctcagtttg aagaaggatt gatagataac ccattccccg gcaaacccgt
    50521 tcagcggcgg cagcgcagcc attgccatta acccgactaa catggcgatg gagataaccg
    50581 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    50641 cgctccccgc cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    50701 gaccaccgac caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgttacgc
    50761 cagcgcccag ccccagcagg ataatgccga tattttccag ggtgtggtaa gccagcaggc
    50821 gctggatgtt gtgctccatc agcgcataca gaccaccgac aaacgcggtg atcatgccga
    50881 gcaccagcag cgcgatcccc caccacagcg gtgcattacc gcccagcagt gacagggtta
    50941 aaatgcccag caggccaatt ttcatgacta ccgtagaaaa caacgcggca gctggcgcag
    51001 aggcgttagc atgtgcctgc ggcacccagc cgtgcagcgg aataatcccg gcgagcaggc
    51061 caaagccaat cacaccgagc agccagatat cggaaccgag cggcagctgt tgcatacgca
    51121 tatccagcag gcgcagatcc agcgtgccat aacgctgcca cagcagccag caagcaatcg
    51181 ccagcagcag agtgccaaga cgccccagcg caaaccacag tttgccctct ttgctgttgc
    51241 tagtgaggaa caccgcgcac agggccatga tttcggccat taccacgaac atgccgaggt
    51301 tgctggcaat gacggcgcag acggcggcag ccatcaacat attgatctgc aagccgttgc
    51361 acttcacctg cgcgtggcga tgccagtcaa tgttatagag gctgacaaac agaccgcaaa
    51421 ggccgagggt aatcagccaa atcgcgttaa gcggggagat ttgcacatcg tagcttacca
    51481 gcgacagcgc accgctcacg ccaaccgcgc cagtcagcac agtgaagccc gcggctgccg
    51541 tatacaggct accaaccgcg ccgccaattc cggctatcca gccacttagc gtcttttgaa
    51601 aagaaaagag aaatgccaga acagcggcgg cgacaaacca cgccacgccg ctattgatca
    51661 gggaaattgc gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc
    51721 aaagtccgtg ttaaaggtca gctcacgctt acgtttgctg acgcgggcga tatcggtgtt
    51781 atccaccaga tgcagggctt tagtcgggca catccgcacg caggccggac cttgttcatc
    51841 aaagctacaa aggtcacatt tgacggcgat cgcgcgaata cctggcaccc agtcaagcaa
    51901 tgtgctgaca cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcgtttgc
    51961 cggaatatcc agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc
    52021 gcacagcttg cagcttacgc acaggctttc attcaactgc acggccccat caacgcgggt
    52081 gatggcgtta accgggcaga ccaccgcgca gggtgcatct tcacagtggt gacagagctg
    52141 cggcgcagat tctttttcat tcagcatcac tctcaggcgc ggcattgatt gcaggccgtg
    52201 ctggcgatgc gtctctgaac aggcggcctc acaagtgtgg cagccgatac agagcgtgga
    52261 gtcagcaatt acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa
    52321 acatgccgat gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta
    52381 acgcctgcaa aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt
    52441 ggcttaaatt caccggcaca ttgttttcaa tagcggtgta taagctgtcg taaacgccag
    52501 cgattttttc gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat
    52561 caatcatgcc gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt
    52621 ttgtttcgac gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa
    52681 tgcgaaaatg ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt
    52741 gatgcaggcg cgctttcgat aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc
    52801 actggtcctg taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc
    52861 aaatagtcat tgtcaggtta cccgtttaac agagatgcca gctttaagca ttttctgtgc
    52921 caacttttaa tttattgtta ttaaagagat ttttaagcta aagatgaatt tcgtcgccgt
    52981 gtcgacgtgt catttcgaca tcatcgacat tattcaccgc agggataatc aacactggca
    53041 caattattgc ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct
    53101 gccaacgggc actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa
    53161 ctggggtctg gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt
    53221 gttttgatct ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac
    53281 aagaggccga atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg
    53341 tccgccgctg tccacagtgt catggtgaca tgctgcagat tgtggcagac gacggtttac
    53401 agattcggcg gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg
    53461 aaggcaacct gtatatcgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc
    53521 catttgcccc ggcggcacgc ccgaagatga aaatcaccgg cattaaagcg cctgaattta
    53581 cccccagcca gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcacg
    53641 caccgggcat gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaata
    53701 accgtctggc tgaacgcaac cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc
    53761 tggtttccag cccaggttcc ggtaaaacca ccctgctgac ggaaacctta atgcgcctga
    53821 aagacagcgt tccgtgcgcg gttattgaag gtgaccagca aaccgtgaac gatgccgcac
    53881 gcattcgcgc taccggcaca ccagcgattc aggtgaacac cggtaaaggc tgccatcttg
    53941 acgcacagat gattgccgac gccgcaccgc gtctgccact ggacgataac ggtattctgt
    54001 ttatcgaaaa cgttggcaac ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca
    54061 aagtggcggt gctttccgtt accgaaggtg aagacaaacc gctgaaatat ccgcatatgt
    54121 ttgccgccgc ctcgctgatg ctgctcaaca aagttgacct gttgccgtat ctcaactttg
    54181 acgttgagaa gtgcctcgcc tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta
    54241 tttccgccac cagcggtgaa gggatggacc agtggctgaa ctggctggag acacagcgat
    54301 gtgcataggc gttcccggcc agatccgcac cattgacggc aaccaggcaa aagtcgacgt
    54361 ctgcggcatt cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca
    54421 gccgcgcgtg ggccagtggg tactggtaca cgttggcttt gccatgagcg taattaatga
    54481 agccgaagca cgcgacactc tcgacgcctt acaaaacatg tttgacgttg agccggatgt
    54541 cggcgcgctg ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg
    54601 gaacaggtga tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacacc
    54661 gccgaacgcc ctctgcggat tatggaagtg tgcggtggtc atacccacgc tatttttaaa
    54721 ttcggcctcg accagttact gcctgaaaac gttgagttta tccacggtcc gggttgcccg
    54781 gtgtgcgtac tgccgatggg tagaatcgac acctgcgtgg agattgccag ccatccggaa
    54841 gtcatcttct gtacctttgg cgacgccatg cgcgtaccag ggaaacaggg atcgctgttg
    54901 caggcaaaag cacgcggtgc cgatgtgcgc atcgtttact cgccgatgga tgcgttgaaa
    54961 ctggcgcagg agaatccaac ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccacc
    55021 atgccgacca ccgccatcac tctgcaacag gcgaaagcgc gtgatgtgca gaatttttac
    55081 ttcttctgcc agcacattac gcttatcccg acgctgcgca gtttgctgga acagccggat
    55141 aacggtatcg acgcgttcct cgcgccgggc cacgttagta tggttatcgg cactgatgcc
    55201 tataatttta tcgccagcga ttttcatcgt ccgctggtgg tcgctggttt cgaacccctt
    55261 gatctactgc aaggcgtggt catgctggtg gagcagaaaa tagcggccca cagcaaggta
    55321 gagaatcagt atcgtcgggt ggtaccggat gccggtaacc tgctggcgca acaggcgatt
    55381 gccgatgtgt tctgtgtcaa cggcgacagc gaatggcgcg gcttaggcgt gattgaatct
    55441 tctggcgtgc acctgacgcc ggattatcaa cgattcgatg ccgaagcaca tttccgcccg
    55501 gcaccgcagc aggtctgcga tgacccgcgc gcgcgttgtg gtgaggtatt aacgggcaaa
    55561 tgtaagccgc atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcatttggt
    55621 gcactgatgg tctcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag
    55681 agtgaagcgt gaataatatc caactcgccc acggcagcgg cggccaggcg atgcagcaat
    55741 taatcaacag cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagatc
    55801 aggcacgtct tgatctggcg cagctggtag cagaaggcga tcgtctggcg ttctccaccg
    55861 acagttacgt cattgacccg ctgttctttc ctggcggtaa tatcggcaag ctggcgattt
    55921 gcggcactgc gaatgacgtt gcggtcagtg gcgctattcc gcgctatctc tcctgtggct
    55981 ttatcctcga agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag
    56041 aaaccgcccg cacggcaggc attgccatcg ttactggcga tactaaagtg gtgcagcgcg
    56101 gcgcggcaga taaactgttt atcaacaccg ctggcatggg cgcaattccg gcgaatattc
    56161 actggggcgc acagacgcta accgcaggcg atgtattact ggttagcggc acgctcggcg
    56221 accacggggc gactatcctt aacctgcgtg agcagctggg gctggatggc gaactggtca
    56281 gcgactgcgc ggtactgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag
    56341 cgctgcgtga tgccacccgt ggtggtgtga acgcggtagt tcatgagttc gcggcagcct
    56401 gcggttgtgg tattgaaatt tcagaagcgg cactgccggt taaacctgcc gtgcgcggcg
    56461 tttgcgaact gctgggactg gacgccctta actttgccaa cgaaggcaaa ctggtgatcg
    56521 ccgttgaacg caacgcggca gagcaagtgc tggcagcgtt acattcccat ccactgggaa
    56581 aagacgcggc gctgattggt gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt
    56641 atggcgtgaa acgaaccctc gatttaccac acgccgaacc gcttccgcgt atatgctaat
    56701 aaaattctaa atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc
    56761 tggaagaaca aaatgtcata tacaccgatg agtgatctcg gacagcaagg gttgttcgac
    56821 atcactcgga cactattgca gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa
    56881 ctggtaaagc gttctgcgct cgccgacaac gcggctattg tgttgtggca agcgcagact
    56941 caacgtgcgt cttattacgc atcgcgtgaa aaagacaccc ccattaaata tgaagacgaa
    57001 actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac gctgcattgc
    57061 agttacgaag aattttgtga aacctggccg cagctgggct ataccccaaa tttggtcact
    57121 attgcctgat gccactggcg gcggaagggc atatttttgg tggctgtgaa tttattcgtt
    57181 atgacgatcg tccctggagc gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg
    57241 tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac tatgagttgt
    57301 tatgccggga gcgcgataac ttccgcatcc tggttgccat taccaacgcg gtgctttccc
    57361 gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac tatttcgaca
    57421 ttgacgatat cagcattgtc ttacgcagcc accgtaaaaa caaactcaac atctactcca
    57481 ctcactatct tgataaacaa catcccgccc acgaacagag cgaagtcgat gaagccggaa
    57541 ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gatcaatctc cacgagcggg
    57601 acgatttagc cccctatgaa cgcatgttgt tcgacacctg gggcaaccag attcaaacct
    57661 tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat
    57721 gcgaagagaa agtgtttacc actaccaatc tgaatttgct gcgccagatt gccgaacgtg
    57781 tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg aaagaacggc
    57841 tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt gaatttggcg
    57901 agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa atggtggcgc
    57961 aaagtgacag taccgtgctg atcctcggtg aaactggcac gggtaaagag ctgattgccc
    58021 gcgcgatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg
    58081 cgatgcctgc cggattgctg gaaagcgatc tgtttggtca tgagcgtggg gcttttaccg
    58141 gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga taaaagctct ctgttcctcg
    58201 acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta ttgcaggaac
    58261 aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtacgt ttaattgccg
    58321 cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc gatctctatt
    58381 accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg gaagatattc
    58441 cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca
    58501 gcattcctgc cgagacgctg cgcaccttga gcaacatgga gtggccgggt aacgtacgcg
    58561 aactggaaaa cgtcattgag cgcgcggtat tgctaacacg cggtaacgtg ctacagctgt
    58621 cattgccaga tattgtttta ccggaacctg aaacgccgcc tgccgcaacg gttgtcgccc
    58681 aggagggcga agatgaatat cagttgattg tgcgcgtgct gaaagaaacc aacggcgtgg
    58741 ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac
    58801 ggatgaagcg gctgggaatt gataaatcgg cattgattta actgcaaatt gccggacaga
    58861 tctgcctgtc cggcatacta ttcacgaggt tttttcggac gatatttttc cggcagttct
    58921 ggcaccggac gcttgtcatc gatgagatga cgcacggtta agatcggatg acgccacagc
    58981 attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc aggctggtaa
    59041 cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca tttatccagc
    59101 cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga cgcctgcggg
    59161 catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg agagatacgc
    59221 ttgccggaca t
//
LOCUS       DAZKGS010000009        58564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKGS010000009 DAZKGS010000000
VERSION     DAZKGS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..480)
                     /gene="folK"
                     /locus_tag="ACHOQY_000588"
     CDS             complement(1..480)
                     /gene="folK"
                     /locus_tag="ACHOQY_000588"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9676740.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLAVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(477..1874)
                     /gene="pcnB"
                     /locus_tag="ACHOQY_000589"
     CDS             complement(477..1874)
                     /gene="pcnB"
                     /locus_tag="ACHOQY_000589"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9676741.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1934..2830)
                     /gene="gluQRS"
                     /locus_tag="ACHOQY_000590"
     CDS             complement(1934..2830)
                     /gene="gluQRS"
                     /locus_tag="ACHOQY_000590"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9676742.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2897..3352)
                     /gene="dksA"
                     /locus_tag="ACHOQY_000591"
     CDS             complement(2897..3352)
                     /gene="dksA"
                     /locus_tag="ACHOQY_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9676743.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3530..4234)
                     /gene="sfsA"
                     /locus_tag="ACHOQY_000592"
     CDS             complement(3530..4234)
                     /gene="sfsA"
                     /locus_tag="ACHOQY_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9676744.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4249..4779)
                     /gene="thpR"
                     /locus_tag="ACHOQY_000593"
     CDS             complement(4249..4779)
                     /gene="thpR"
                     /locus_tag="ACHOQY_000593"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9676745.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4853..7282
                     /gene="hrpB"
                     /locus_tag="ACHOQY_000594"
     CDS             4853..7282
                     /gene="hrpB"
                     /locus_tag="ACHOQY_000594"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9676746.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7414..9948
                     /gene="mrcB"
                     /locus_tag="ACHOQY_000595"
     CDS             7414..9948
                     /gene="mrcB"
                     /locus_tag="ACHOQY_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9676747.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10168..12411
                     /gene="fhuA"
                     /locus_tag="ACHOQY_000596"
     CDS             10168..12411
                     /gene="fhuA"
                     /locus_tag="ACHOQY_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9676748.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TVAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12462..13259
                     /gene="fhuC"
                     /locus_tag="ACHOQY_000597"
     CDS             12462..13259
                     /gene="fhuC"
                     /locus_tag="ACHOQY_000597"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9676749.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13259..14149
                     /gene="fhuD"
                     /locus_tag="ACHOQY_000598"
     CDS             13259..14149
                     /gene="fhuD"
                     /locus_tag="ACHOQY_000598"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9676750.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14146..16128
                     /gene="fhuB"
                     /locus_tag="ACHOQY_000599"
     CDS             14146..16128
                     /gene="fhuB"
                     /locus_tag="ACHOQY_000599"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP9676751.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16163..17443)
                     /gene="hemL"
                     /locus_tag="ACHOQY_000600"
     CDS             complement(16163..17443)
                     /gene="hemL"
                     /locus_tag="ACHOQY_000600"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9676752.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17609..17656
                     /gene="yadX"
                     /locus_tag="ACHOQY_000601"
     CDS             17609..17656
                     /gene="yadX"
                     /locus_tag="ACHOQY_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9676753.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17668..19089
                     /gene="clcA"
                     /locus_tag="ACHOQY_000602"
     CDS             17668..19089
                     /gene="clcA"
                     /locus_tag="ACHOQY_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9676754.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19113..19178
                     /gene="yadW"
                     /locus_tag="ACHOQY_000603"
     CDS             19113..19178
                     /gene="yadW"
                     /locus_tag="ACHOQY_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9676755.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19171..19515
                     /gene="erpA"
                     /locus_tag="ACHOQY_000604"
     CDS             19171..19515
                     /gene="erpA"
                     /locus_tag="ACHOQY_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9676756.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19562..20185)
                     /gene="yadS"
                     /locus_tag="ACHOQY_000605"
     CDS             complement(19562..20185)
                     /gene="yadS"
                     /locus_tag="ACHOQY_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9676757.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20223..21023)
                     /gene="btuF"
                     /locus_tag="ACHOQY_000606"
     CDS             complement(20223..21023)
                     /gene="btuF"
                     /locus_tag="ACHOQY_000606"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9676758.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21016..21714)
                     /gene="mtnN"
                     /locus_tag="ACHOQY_000607"
     CDS             complement(21016..21714)
                     /gene="mtnN"
                     /locus_tag="ACHOQY_000607"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9676759.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21798..23315
                     /gene="dgt"
                     /locus_tag="ACHOQY_000608"
     CDS             21798..23315
                     /gene="dgt"
                     /locus_tag="ACHOQY_000608"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9676760.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23445..24869
                     /gene="degP"
                     /locus_tag="ACHOQY_000609"
     CDS             23445..24869
                     /gene="degP"
                     /locus_tag="ACHOQY_000609"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9676761.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25026..26177
                     /gene="cdaR"
                     /locus_tag="ACHOQY_000610"
                     /pseudo
     CDS             25026..26177
                     /gene="cdaR"
                     /locus_tag="ACHOQY_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26266..26652)
                     /gene="yaeH"
                     /locus_tag="ACHOQY_000611"
     CDS             complement(26266..26652)
                     /gene="yaeH"
                     /locus_tag="ACHOQY_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9676762.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26814..27626)
                     /gene="yaeI"
                     /locus_tag="ACHOQY_000612"
     CDS             complement(26814..27626)
                     /gene="yaeI"
                     /locus_tag="ACHOQY_000612"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFP9676763.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27681..28505)
                     /gene="dapD"
                     /locus_tag="ACHOQY_000613"
     CDS             complement(27681..28505)
                     /gene="dapD"
                     /locus_tag="ACHOQY_000613"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9676764.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28536..31208)
                     /gene="glnD"
                     /locus_tag="ACHOQY_000614"
     CDS             complement(28536..31208)
                     /gene="glnD"
                     /locus_tag="ACHOQY_000614"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9676765.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31270..32064)
                     /gene="map"
                     /locus_tag="ACHOQY_000615"
     CDS             complement(31270..32064)
                     /gene="map"
                     /locus_tag="ACHOQY_000615"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9676766.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32432..33157
                     /gene="rpsB"
                     /locus_tag="ACHOQY_000616"
     CDS             32432..33157
                     /gene="rpsB"
                     /locus_tag="ACHOQY_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9676767.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33415..34266
                     /gene="tsf"
                     /locus_tag="ACHOQY_000617"
     CDS             33415..34266
                     /gene="tsf"
                     /locus_tag="ACHOQY_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9676768.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34413..35138
                     /gene="pyrH"
                     /locus_tag="ACHOQY_000618"
     CDS             34413..35138
                     /gene="pyrH"
                     /locus_tag="ACHOQY_000618"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9676769.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35430..35987
                     /gene="frr"
                     /locus_tag="ACHOQY_000619"
     CDS             35430..35987
                     /gene="frr"
                     /locus_tag="ACHOQY_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9676770.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36079..37275
                     /gene="ispC"
                     /locus_tag="ACHOQY_000620"
     CDS             36079..37275
                     /gene="ispC"
                     /locus_tag="ACHOQY_000620"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9676771.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37464..38222
                     /gene="ispU"
                     /locus_tag="ACHOQY_000621"
                     /gene_synonym="uppS"
     CDS             37464..38222
                     /gene="ispU"
                     /locus_tag="ACHOQY_000621"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9676772.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38235..39092
                     /gene="cdsA"
                     /locus_tag="ACHOQY_000622"
     CDS             38235..39092
                     /gene="cdsA"
                     /locus_tag="ACHOQY_000622"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9676773.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39104..40456
                     /gene="rseP"
                     /locus_tag="ACHOQY_000623"
     CDS             39104..40456
                     /gene="rseP"
                     /locus_tag="ACHOQY_000623"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9676774.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40486..42918
                     /gene="bamA"
                     /locus_tag="ACHOQY_000624"
     CDS             40486..42918
                     /gene="bamA"
                     /locus_tag="ACHOQY_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9676775.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGEQYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43040..43525
                     /gene="skp"
                     /locus_tag="ACHOQY_000625"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43040..43525
                     /gene="skp"
                     /locus_tag="ACHOQY_000625"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9676776.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43529..44554
                     /gene="lpxD"
                     /locus_tag="ACHOQY_000626"
     CDS             43529..44554
                     /gene="lpxD"
                     /locus_tag="ACHOQY_000626"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9676777.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44659..45114
                     /gene="fabZ"
                     /locus_tag="ACHOQY_000627"
     CDS             44659..45114
                     /gene="fabZ"
                     /locus_tag="ACHOQY_000627"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9676778.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45118..45906
                     /gene="lpxA"
                     /locus_tag="ACHOQY_000628"
     CDS             45118..45906
                     /gene="lpxA"
                     /locus_tag="ACHOQY_000628"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9676779.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45906..47054
                     /gene="lpxB"
                     /locus_tag="ACHOQY_000629"
     CDS             45906..47054
                     /gene="lpxB"
                     /locus_tag="ACHOQY_000629"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9676780.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47051..47647
                     /gene="rnhB"
                     /locus_tag="ACHOQY_000630"
     CDS             47051..47647
                     /gene="rnhB"
                     /locus_tag="ACHOQY_000630"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9676781.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47684..51166
                     /gene="dnaE"
                     /locus_tag="ACHOQY_000631"
     CDS             47684..51166
                     /gene="dnaE"
                     /locus_tag="ACHOQY_000631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9676782.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51179..52138
                     /gene="accA"
                     /locus_tag="ACHOQY_000632"
     CDS             51179..52138
                     /gene="accA"
                     /locus_tag="ACHOQY_000632"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9676783.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52237..54378
                     /gene="ldcC"
                     /locus_tag="ACHOQY_000633"
     CDS             52237..54378
                     /gene="ldcC"
                     /locus_tag="ACHOQY_000633"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9676784.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54435..54824
                     /gene="yaeR"
                     /locus_tag="ACHOQY_000634"
     CDS             54435..54824
                     /gene="yaeR"
                     /locus_tag="ACHOQY_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9676785.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54889..56187
                     /gene="tilS"
                     /locus_tag="ACHOQY_000635"
     CDS             54889..56187
                     /gene="tilS"
                     /locus_tag="ACHOQY_000635"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9676786.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56236..56496)
                     /gene="rof"
                     /locus_tag="ACHOQY_000636"
     CDS             complement(56236..56496)
                     /gene="rof"
                     /locus_tag="ACHOQY_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9676787.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56483..56683)
                     /gene="yaeP"
                     /locus_tag="ACHOQY_000637"
     CDS             complement(56483..56683)
                     /gene="yaeP"
                     /locus_tag="ACHOQY_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9676788.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56849..57394
                     /gene="yaeQ"
                     /locus_tag="ACHOQY_000638"
     CDS             56849..57394
                     /gene="yaeQ"
                     /locus_tag="ACHOQY_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP9676789.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57391..57813
                     /gene="arfB"
                     /locus_tag="ACHOQY_000639"
     CDS             57391..57813
                     /gene="arfB"
                     /locus_tag="ACHOQY_000639"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9676790.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57827..58537
                     /gene="nlpE"
                     /locus_tag="ACHOQY_000640"
                     /gene_synonym="cutF"
     CDS             57827..58537
                     /gene="nlpE"
                     /locus_tag="ACHOQY_000640"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9676791.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13745 a        14545 c        16309 g        13965 t
ORIGIN      
        1 ttaccatttg cttaatttgt caaatgctct tgtatgtaag acttcacgca acgtctcccc
       61 atcaggaaac gccagctccg gtgcaatctc aaacagcggc cacagcataa atccacgatt
      121 cttcatatcg tagtgcggaa cggccaggcg ttcggtattt atcacttcat taccaaacag
      181 catgatatcg agatccagcg tgcgtggtcc ccagcgttca gctttacgaa cacgaccctg
      241 ttgcagttct atgcgctgtg tatggttgag taactcttcc ggggcgagcg aagtttcaag
      301 cgccacggcg gcgttgaggt aatcgggttg atcttgcggc cccagcggtg gggtgcggta
      361 aaacgaagaa acggcaagaa tgtggctttc agggatatcg cctaatgctt tcagggcagc
      421 attgacctgc tccagcggag aggccagatt gctgcctatg gcaatatacg ccactgtcat
      481 gcggtaccct cacgacgcgg tgcgcgtttg cgtggacgac gagtacggcg acgcggtgac
      541 ggctcttcat ccaactcgtt aagcatccct ttttgatctg gtggcgcgga aacctggaac
      601 tcaccccacc atttcaccag acgctgcagt tcagcgttac gttcaacttc agctcgcaag
      661 gccaacaggt cataagccgc acggaactta ggatgctcca gcagtttcca tgcgcgttta
      721 ccctgacgac gggacatacg caactgcaac tgccagatat cgcgggttaa cgtcgtcaga
      781 cgtttcggga ttgccagtga acggcaggct tcgtccagca cgtcgttcat cgccagcgcg
      841 aaagcgtcgt gataggtcag gccgctttcc tgggcaatct tctgtgccgt ctccagcagt
      901 gggtaccaga acatggcggc aaacaggaac gccgggttca cgcgcatatc gttatggata
      961 cgcgtatcgg tattcttcag cacctgttca atgatccgct ccatcgggct gtcgccattt
     1021 tccgtgaagt agcgggtaat ggtcgggaac agcggctgga acagatgata ttcacacaac
     1081 agcttatagg tatcgtaacc gtagcccgct tgtagcagtt taagcgattc ttcaaacagg
     1141 cgtgccggtg ggatatcgtt caacagggta gcgaggcgag ggatcggttc tgcggtttcc
     1201 gggctgatgc gcatacccaa tttggcggca aaacgtaccg cgcgcagcat acgtaccgga
     1261 tcttcacggt agcgcgtttc cgggttacca atcagacgga taacgccgtc cttcagatcc
     1321 ttcataccgc caacgtaatc acggacggta aaatccgcta cgctgtaata gaggctgttg
     1381 atagtgaaat cgcggcgctg ggcgtcttct tcgatggagc cgaaaatgtt gtcgcgcagc
     1441 aacatgccgt tttgcccgcg ttgggaggtc gtgcggtcgc tgacgttacc ttcgtggtgt
     1501 ccacggaagg tcgcaacttc gataatctcc gggccaaaca ttacatgagc cagacggaaa
     1561 cggcgaccca ccaggcggca gttacggaac agtttgcgca cctgctcagg cgtggcgtta
     1621 gtggttacgt caaaatcttt cggctttttg ccaagtaaca ggtcgcgcac gccgccgcca
     1681 accagccagg cttcgtatcc cgctttgttg agcctgtaca ttaccttcag ggcattttca
     1741 ctgatatctt tgcgggaaat agcatgctgc tcacgcggga tcaccgtcac ctgtggacgg
     1801 gcgactgcct gttcagcctc gctttcctcg cggcttagca ccttgcggca aaaattagcg
     1861 actcgggtaa aaatagtaca cctcggtagt gtcaaacatc attcaggaca aaaaaatagc
     1921 ggctaatcat agctcagcat gacgcatttg agaatgttga atttacaatt gccgactcgg
     1981 gcacggcggt aagccgccag tttttgacgg ctgactgaag gatttgctcg acgctgaaat
     2041 cctgccagtg tgcttctgcc tgctgcccca gaaattgaag tgccgcgatt agtaccgggc
     2101 gtggatcgcc tttcggcaac gcaggcgcat gattctgctt ggaaagttta gcgccttgtg
     2161 gattaagcgc cagcggcaga tgaatgtaat ctggcacttt ccagccaaaa agctggtaca
     2221 gcgagatttg ccttactgtt ggttcaatca gatcagcccc acgcactatt tctgtaacgc
     2281 cctggaaatg atcatcaacc acaacagcca ggttgtaggc gaacaaccca tcacggcgat
     2341 gaatgataaa atcttcccgt gccagttttt cgtcggcgtg aataatgccg cgcagctggt
     2401 cagtaaattg cgtgaccgga tgctgctggc ggatacgcac tgcggcattg tctggtccat
     2461 gatgcaacac ccggcaatga ccgtcgtaaa taccgccaat gctttgaata cgcgcacgcg
     2521 tacaggtgca gtaataactt agtccttgtt catgtaacca ggcgagtgct tcacgatagg
     2581 cgtggtgacg ttgcgattgc cagagaacat cgccgtccca gtgcagaccg taatgttcca
     2641 gctggcgcag gatagtttct gcggcaccgg gaacttcacg aggcgggtcg atatcttcta
     2701 tgcgtaccag ccagcgacct tgccgggcgc gagcctgcaa atagctgccg agcgcggcga
     2761 tcagagagcc aaaatgaagc tcgccggaag gagagggggc gaagcggcca atatactgtg
     2821 tgtctgtcat ctctttgaac aaaaaataag gcgggagcat ttcccgcctg tggtaaacgt
     2881 gatggaacgg ctgtaattag ccagccatct gtttttcgcg aatttcagcc agcgttttgc
     2941 agtcgatgca cagatcggct gtcgggcgcg cttccagacg gcgaatacca atttcaacac
     3001 cgcaggattc gcagtagccg aaatcttcgt cttccacttt tttcagcgtc ttctcgatct
     3061 ttttgatcag cttacgctcg cgatcgcggt tacgcagttc gaggctgaac tcttcttcct
     3121 gggctgcacg gtctaccggg tccgggaagt tggctgcttc atcctgcata tgtgtaacgg
     3181 tgcgatcgac ttcatccctg agttgattac gccatgcttc cagaatacga cggaagtgcg
     3241 ccagctgggc ttcattcata tactcttcgc ccggcttctc ctgatatggt tccaccccag
     3301 cgatggcgag aatactcagg gacgatgttt tacggttttg cccttcttgc atgttgcttc
     3361 tccttaacac gcactatcga tccccatgtt cgggggaaaa atgaggccgc tataaatagc
     3421 agatgctttt ccggatagca attatctaaa cgtaacactt gacaactgtg tgaggaaaag
     3481 cgtatttgcg cacgcgacca gaatgtaaat taaccagtta cttactttac tacaatgtaa
     3541 ccggcagtga ttttttaaga gccatgcctt cagcagaaat ttccgctttg taagccagaa
     3601 tttctacccc cctctgttga gcttctgaca atagttgcgc gtatttctca tcgatgtggc
     3661 gcgcgggtga aaaccgtgta atggctgaat gcagcacggc gaaaaagata accgcacgct
     3721 ggccttcagc cgctacgctc atcaactccc gaaggtgttt ctgacctcgt tcagtgaccg
     3781 catcgggaaa atatccctgt tcgttctccg ctaacgtaac cgatttcact tcaatatagc
     3841 agtctggacg cgaatccgcc tgcaacataa agtcaatacg gctgcgttct gcgccgtatt
     3901 ttacttcgct tttcagcgag ctatagcctg acagttctga aattgattca ttaaggatag
     3961 cctctttcgt caacctgtta gcccaaagcg tgttgacgca aataaatgcg ccgctctggc
     4021 tttgagttaa ttcccaggtg tgtgggtatt tccgtttggt gttgtctgaa gtcgaatacc
     4081 agacggtatc gccaggcgtt gcacaaccgg tcattgcacc cgtattcggg cagtgtagcg
     4141 ttaattcgcg accatcgggt gtgatcacat cggctaaaaa acgtttgtaa cgctgaatta
     4201 gcgtcgcgcg ctgtagaggg ggagaaaatt ccattgcgac aatccttgtt attgcgttag
     4261 cgcccagcgt tttagcggcg tgtagcgtgt gcgtccacgg gcaaacgagg aggcgtaaag
     4321 ggtgaactcc gtcaccgcat acgaccagtt aaaacctggc ggcgggattg tcaccgcctc
     4381 gctggcgtcg cgcaataagg taatatgtgg atgaaacgga cgattgcttt gaaaacaacc
     4441 gctgcgggca gcctgtgaac ggagcatatt cgccagctgg attaagccgc gtggcggctg
     4501 acgcatccct aaccacacca cacgcgaacg cagccattgt ccggcgtcat caagcgtgag
     4561 tgtgaaacca ggttgacgaa tccgtccggc taaaagagaa agcgccttct ctttctcggc
     4621 gctcacttcg cctaaaaatg ccagagtcag atgcaaatta tcggcggcga ccggacgtcc
     4681 cgcctcaggt gggaagtgtg tggcgcgcca gtggataatc tgttcgcgga tttctgcagg
     4741 taagtcgata gcaaagaaca gacgttgcgg ttcagacatg tgaggcactc ggttatgaat
     4801 taccgcgatg ctacaatgtg gcgcgaagaa tgttaaccct ctggagcgtt ttgtgtcgtc
     4861 gttgcccgtt gctgccgtct tacctgaatt acttaccgct ctcgattgtg cgccgcaggt
     4921 attattaagt gcgccgaccg gggccgggaa atcaacctgg ctgccgctgc aactgctggc
     4981 gcatcccggc attaacggga aaattatcct gctggagccg cgtcgtctgg cggcgcgtaa
     5041 cgtcgcgcaa cggctggcgg agctgcttaa cgaaaagcca ggcgataccg ttggctaccg
     5101 gatgcgtgcg caaaactgcg tcgggccgaa tacccgcctg gaagtagtta ccgaaggcgt
     5161 gctgacgcgg atgatccagc gtgacccgga gttgagcggt gttggattgg tgatcctcga
     5221 tgagtttcat gagcgcagct tgcaggcgga tttggcgttg gcgctgttac tcgatgtgca
     5281 acaaggtctg cgtgatgacc ttaaactgct gattatgtcg gctacgctgg acaacgaccg
     5341 cttgcagcaa atgctgccag aagcgcccgt cgtcatctca gaagggcgct cgtttccggt
     5401 tgaacgccgt tatttaccac tgcccacgca tcagcgtttt gacgaagccg ttgcggtagc
     5461 caccgctgaa atgctgcgtc aggaaagtgg atcattactg ttatttttac ctggcgtcgg
     5521 agaaattcag cgtgtgcagg aacaactggc ttcgcgcatc ggcagcgatg tattgctctg
     5581 cccgctgtat ggcgcgttgt cgctgaacga tcagcgaaaa gcgatcctcc cggcaccgca
     5641 agggatgcgc aaagtggtgc tggcgaccaa tattgctgaa accagtttaa ccatcgaagg
     5701 tattcgtctg gtggtggatt gtgcccagga gcgtgtggcg cgttttgatc cgcgcacggg
     5761 gcttacgcga ctgattactc aacgcgttag ccaggcatcc atgacgcagc gtgccgggcg
     5821 cgccgggcgt ctggagccgg gtatctgcct gcatttaatc gccaaggaac aagcagaacg
     5881 cgccgcggcg caaagtgaac cggagatatt acaaagcgat ctttccggtt tgctgatgga
     5941 attactgcaa tggggatgca gcgatccggc gcagatgagc tggctggatc aaccgccagt
     6001 agtgaatcta ctggccgcga aacgtctgtt acaaatgctg ggggcactgg agggtgaacg
     6061 gcttagtgcg caagggcaaa aaatggcagc gctgggtaac gatccgcgtt tagcggcaat
     6121 gctggttagc gcgaagaacg acgacgaagc tgctaccgcg gcaaaaattg ccgccattct
     6181 cgaagagccg ccacggatgg gcaatagtga cctgggcgtg gcgttttcgc gcaatcaacc
     6241 agcctggcag caacgtagtc agcaactgtt aaaacgctta aacgtacgtg gcggtgaggc
     6301 agacagttcg cttatcgcgc cgctacttgc cggggcgttt gccgatcgca ttgctcgtcg
     6361 ccgtgggcaa gatggacgct atcaactggc aaacggcatg ggagcgatgc tcgatgccaa
     6421 cgacgcgcta agccgccacg aatggttgat cgcaccgtta ttattgcagg gcagcgcctc
     6481 gccggatgcg cggattttac tggcgctgcc ggtcgatatt gatgagttag tacaacgctg
     6541 cccgcagctg gtacagcagt ctgacactgt ggagtgggat gacgcgcaag gtacgctgaa
     6601 agcctggcgt cggctgcaaa tcggtctgct gacggtgaaa gtgcagccgc tggcgaaacc
     6661 ctcggaagac gagttgcatc aggcgatgct taatggcatc cgcgataaag atttaagcgt
     6721 gctcaactgg acggcggaag cggaacagct acgcttgcgt ttgttatgcg ccgcaaagtg
     6781 gttgccggaa tatgactggc cagcggttga tgatgaaagt ttattggcga cgctggaaac
     6841 gtggctgctg ccacatatga ctggcgtaca ttcactacgc ggcctgaaat cactcgacat
     6901 ttatcaggca ctacgcggat tacttgattg gggaatgcag caacgtctgg atagtgaatt
     6961 gcctgcgcat tacactgtgc cgacgggaag ccggatcgcc attcgttatc atgaagataa
     7021 cccgcccgcg ctggcggtga gaatgcaaga gatgtttggc gaggccacca atccgacgat
     7081 cgcccagggg cgcgtgccgc tggtgctgga gttgctttca cctgcccaaa ggccattaca
     7141 aatcacacga gatttgagcg ccttctggaa aggagcgtac cgtgaggtgc aaaaagagat
     7201 gaaagggcgt tatcccaaac atgtctggcc ggacgacccg gcaaatactg caccgacgcg
     7261 acggacgaaa aagtattcgt aaggttggtt ttctccctct ccctgtggga gaggggaccg
     7321 atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa cagaaaatcg
     7381 ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg cgagccaatt
     7441 ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg tcgttacgaa
     7501 gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat gccgcgcaaa
     7561 ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct atggctgctg
     7621 ttaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta tctcgatcaa
     7681 aaaattcgta gccgtattga tggcaaggtc tggcaactgc ctgcggcagt ttatggccga
     7741 atggtcaacc ttgagccaga tatgaccatc agcaagaacg agatggtgaa gctgctggag
     7801 gcgactcagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac cgtgcaggcc
     7861 aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga agggcaggtg
     7921 cgtgcgcgtc tgacctttga tggcgatcat ctggcgacga tcgtcaatat ggagaacaac
     7981 cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc ctcgccaaac
     8041 ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt ggatactttg
     8101 ctggcgacag aagaccgtca tttttacgag catgacggca tcagcctcta ctccatcgga
     8161 cgtgcggtgc tggcaaacct gaccgccgga cgcacggtgc agggagcgag tacgctgacg
     8221 caacagctgg tgaaaaacct gttcctctcc agtgagcgtt cttactggcg taaagcgaac
     8281 gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat tcttgagctg
     8341 tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg
     8401 gcgagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca gcaggcgctg
     8461 ttagtcggca tggtgaaagg agcgtccatc tacaacccgt ggcgtaaccc aaaactggcg
     8521 ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat tgatcaagaa
     8581 ctctatgaca tgttgagtgc acgtccgctg ggtgttcagc cacgcggtgg ggtgatctct
     8641 ccacagccag cctttatgca actggtgcgt caggagctgc aggcgaaatt aggcgataag
     8701 gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc ccaggacgcg
     8761 gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa gttgagcgat
     8821 cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc gatggtcggt
     8881 ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt
     8941 tcccttgcaa aaccagcgac ttatctgact gccttaagcc agccgaaaat ctatcgtctg
     9001 aatacgtgga ttgcggatgc gccaatcgcg ctgcgtcagc cgaatggcca ggtctggtca
     9061 ccgcagaatg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt ggatgcgttg
     9121 acccgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct gcctgcggtt
     9181 acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt tccggcgatg
     9241 ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcgttcca gaccatcgcc
     9301 agcggcggta accgtgcacc gctttctgca ctgcgttcgg taattgcgga agatggcaag
     9361 gtgctgtatc agagcttccc gcaggcggaa cgcgctgttc cggcgcaggc ggcgtatctg
     9421 acactatgga ccatgcagca ggtggtacaa cgcggtacgg gtcgtcagct tggggcgaaa
     9481 tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt agatacctgg
     9541 tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga taacaaccag
     9601 ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta tctggctaac
     9661 cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat gggcgtggac
     9721 tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg gaccagcgat
     9781 ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa tccgtttgat
     9841 cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga gcagaaagac
     9901 agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca tctaagcgtg
     9961 aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga tattaaccct
    10021 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
    10081 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
    10141 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
    10201 tcactgcgta aaatcgcagt tgtagtagcc acagcggtaa gcggcatgtc tgtttatgca
    10261 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgttgcacc tgcgccgcaa
    10321 gaaagcgcat gggggcctgc tgcaactatt gcggcgcgac agtctgctac cggcactaaa
    10381 accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc cgaagagatg
    10441 gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg tgtctctgtt
    10501 ggtacgcgtg gcgcatccaa cacctatgac cacctgatca ttcgcggctt tgcggcagaa
    10561 ggccaaagcc agaataacta tctgaatggc ctgaagttgc agggcaactt ctataacgat
    10621 gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc ggtttccgtg
    10681 ctttacggta aaagcagtcc tggcggcctg ttgaatatgg tcagcaagcg tccgaccacc
    10741 gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca gactggtttt
    10801 gactttagcg attcgttgga tgatgacggt gtttactctt atcgcctgac cggtcttgcg
    10861 cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat tgcaccggcg
    10921 ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt ccagaacgag
    10981 ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc gctgccgaac
    11041 ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta ttctcgtaat
    11101 gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac tgtgcgtcag
    11161 aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta cggcgtctgc
    11221 tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc ggataaaggc
    11281 cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt ctccgttgat
    11341 acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct gtccggtgtc
    11401 gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga ctctgtgcca
    11461 ctgctcaatc tgtacaatcc ggtgaatacc gatttcgact tcaatgccaa agatccggca
    11521 aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta tgttcaggat
    11581 caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg ggcagatcaa
    11641 gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca gtttacctgg
    11701 cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag ctatagcgaa
    11761 tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc gtctaaaggt
    11821 aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt agttactggt
    11881 gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg ttccttcttc
    11941 tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa agcggcgctg
    12001 tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata caccaccgat
    12061 actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc gctgtgggct
    12121 gactatacct tctttgacgg tccgctttca ggtctgacgc tgggcaccgg tggtcgttat
    12181 actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta tacggtcgtg
    12241 gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa cgtggcgctg
    12301 catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac ttatggctgc
    12361 ttctggggcg cagaacgtca ggtcgttgca accgcaacct tccgtttcta atttctcttt
    12421 tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa tacacgaatc
    12481 attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg cgcacgcttt
    12541 tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt ggtcacaacg
    12601 gttctggtaa atccactctg ctcaaaatgc ttggccgtca tcagcctccg tcagaagggg
    12661 agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt gcccgcaaag
    12721 tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt gaactggtgg
    12781 cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca gatcgcgaaa
    12841 aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg ctggtcgata
    12901 gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg caggatagcc
    12961 gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag gttgatgtgc
    13021 tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc gtgctgcacg
    13081 atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcggt ggtgaaatga
    13141 ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg atttatggca
    13201 tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt gtttattgat
    13261 gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc tttctccgtt
    13321 gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta ttgtggcgct
    13381 ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg gcgtggcgga
    13441 taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag tgattgacgt
    13501 cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt cgtttatggt
    13561 ctggtcggca ggatatggcc cttcaccaga aatgctggca cgtattgcgc cgggtcgcgg
    13621 atttaacttc agtgacggca aacagccgtt ggcgatggcg cgtaaatcgc tgacggaaat
    13681 ggcagattta cttaacctgc aaagcgcagc agaaacgcat ttagcgcact atgaagactt
    13741 tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac tgctgacgac
    13801 gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc aggaaattct
    13861 cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg gcagtaccgc
    13921 cgtcagtatc gatcgtctgg cggcgtataa agacgttgat gtgctctgtt ttgatcacga
    13981 caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca tgccgtttgt
    14041 ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa cgctttcggc
    14101 aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag taaacgaatt
    14161 gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc gctaacctgg
    14221 atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg gtcgccggat
    14281 attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct ggcgatttcg
    14341 ctgctggtgg gcgcaggcct ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaat
    14401 ccgctggcgg aaccgacgac acttggcgtt gctacaggcg cgcaactggg aattaccgtc
    14461 accacacttt gggcgatccc aggtgctatg gcgagccagt ttgccgcact ggtgggcgct
    14521 tgtgtcgttg gcttaatcgt ctttggcgtc gcgtggggga aacggctttc gccggttacg
    14581 cttattctcg cggggctggt ggtgagcctt tattgcgggg caattaatca gctactggtt
    14641 atcttccatc acgaccagct gcaaagcatg ttcctgtgga gcaccggaac gctgacgcaa
    14701 accgactggg gcggcgttga gcgtttatgg ccgcagttgc tgggcggcgt gatgttgacg
    14761 ttattgctac ttcgcccgtt aaccctgatg gggcttgatg atggcgtggc gcgcaatctc
    14821 gggctggcct tgtcgctcgc gcgtctggca gcgctgtcgc tggcgattgt catcagtgcg
    14881 ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc gctgctggca
    14941 aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgct gattggggcg
    15001 ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg gatggaagtg
    15061 tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct gttgccgcgt
    15121 ttacgcagca ttagcgcgcc ggatatgaag gtcaacgatc gtgtcgcggc tgaacgccaa
    15181 catgtgctgg cgtttgccct cgcgggcggc gtgctgctgt tgatggctgt ggtggtggcg
    15241 ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt gcttgaggat
    15301 ttgatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt catgctggcg
    15361 gtggcgggct gtattattca gcggctgacc ggaaacccga tggcaagccc ggaagtgctg
    15421 gggattagct ctggcgcagc gtttggcgtg gtgttgatgc tgtttctggt gccgggtaat
    15481 gcctttggct ggctgttacc tgcaggcagt ctcggcgcgg cggtgacgct gttgatcatt
    15541 atgatcgccg ccggccgcgg tggattttcc ccacaccgca tgttactggc ggggatggcg
    15601 ttaagcaccg cgttcaccat gcttttgatg atgttgcagg caagtggtga cccgcgaatg
    15661 gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc gcaggtctgg
    15721 cgcacgggaa ttgtgatggt gattttgctg gcgattaccc cgctgtgccg ccgctggctg
    15781 accattttac cgctgggtgg tgataccgcc cgagccgtag gaatggcgct gacgccgacg
    15841 cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat gactattgga
    15901 ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg ctttcgacgg
    15961 acgatgccac acatcgtaat ttcggcgctg gtgggtggtt tactgctggt gttcgctgac
    16021 tggtgtgggc ggatggtgct gtttccattc cagatcccag cagggctgct gtcgaccttt
    16081 atcggcgcgc catattttat ctatttgttg agaaagcaga gccgttaata gaatgcctcg
    16141 cccggtgggc gaggcaaaat catcacaact tcgcaaacac ccgtcgtgca gcatcgatgg
    16201 tgttattgat atcttccatg ctgtgcgcca cggacataaa gcccgcttca aacgctgacg
    16261 gtgccaggta aacaccttcg tccagcatca tatggaagaa acgcttaaag cgttccacgt
    16321 cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag aaaataccga
    16381 acatgccgcc aacgtggtta acgaccagcg gaattccggc ttcttctgcc gcttccagca
    16441 gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaacgccc ggctgcgcga
    16501 cttcattcag acaggcgaaa cccgctgcca tcgcaattgg gttaccggaa agcgtacccg
    16561 cctgatagac cggacccgtc ggggccagcg catccattac atcacgacga ccaccgaatg
    16621 cgcctaccgg cattccaccg ccgatgattt tgcccaggca ggtgagatcc ggttccacac
    16681 cgtaataatc ctgtgcgcca gctagcgcta cgcggaagcc agtcattact tcatcgatga
    16741 tcagcaatgc gccaaattcg tcgcacagcg cgcgcagacc tggcaggaac tctggcagcg
    16801 gtggaacgca gttcatattg cctgccaccg gctcgacgat aatacaggca atctcttgcg
    16861 ggtattgctc aaacgcggcg cgtacagaag ccagatcgtt ataagtacag gttaaggtat
    16921 gtttggcgaa atctgccgga acacccggcg agtttggctg gcctaacgtg agtgcgccag
    16981 aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca aatttaatga
    17041 ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc gcttcagtgc
    17101 cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc agttgcgcca
    17161 ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca
    17221 cggcattgcg gatagccgga tggttatggc ccagcaccat cggcccccag gaaccgacat
    17281 aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct ttttcgataa
    17341 acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga
    17401 tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt tcctgattcg
    17461 tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata ctcgtcaaac
    17521 atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt ttgtgcattt
    17581 gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta
    17641 tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt agaaacaccg
    17701 caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg cgataaaacc
    17761 ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct ggcagcggtt
    17821 gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct ggtacatact
    17881 gctgataatt atccgcttct gttaaccgtc gcttttctct gttcggcggt gctggcgatg
    17941 ttcggctact ttttggtgcg caaatacgcg ccggaagcag gtggttcggg gatcccggaa
    18001 attgaagggg cgctggaaga tcaacgtccc gttcgctggt ggcgtgtgtt gccggtgaaa
    18061 ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga aggaccaacc
    18121 gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct gaaaggtgat
    18181 ggagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc ggcctttaac
    18241 gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt tcgctatacg
    18301 ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat gtaccggatt
    18361 tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg
    18421 ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat ttttaataaa
    18481 tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg
    18541 gtactgatgg gtggtgcgat tggcggtctg tgtggattgc tggggtttgt ggcaccagca
    18601 acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt cagcatggga
    18661 atgctggtgt ttattttcgt cgctcgggtc attaccacct tactctgctt ctcttccggc
    18721 gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg aaccgctttc
    18781 ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac gtttgctatt
    18841 gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg gatcattctg
    18901 gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac cggtcttggc
    18961 gcaacactat tagcgcaatt taccggcggg aaaccgctat actcggcgat tcttgcgcgc
    19021 acgctggcaa aacaggaagc tatgcaactg gcgcgaagca aggccgcatc agccagcgag
    19081 aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg ttagaatttg
    19141 cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact gccgctggag
    19201 tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga taacccgaat
    19261 ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta tggtttcacc
    19321 tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt tggcttggtg
    19381 gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactatac cgaaggactg
    19441 gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct
    19501 tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac atcttctctc
    19561 ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa cgaatagccg
    19621 ccagccgaat caagagcgtc acgaccatgc ccatcatact ggctgtttcc agtggtacgg
    19681 aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca gttgcgtaga
    19741 tttctgtacg taaaatcatg gggatttcgc gggccagaac atcacgaatg atcccgccgc
    19801 caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc gcattaaagg
    19861 ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc accggcaaca
    19921 tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg ctggttacca
    19981 ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga tccagcgcca
    20041 tgtcgcgaat tgtcccgccg cctacagcgg taaccacgcc cagtaccaga acaccaaaag
    20101 ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc gctgtgccga
    20161 ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga aaaggtcagg
    20221 cactaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat aatacgtggg
    20281 cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag ctgttcaccc
    20341 cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat cgcctgtggc
    20401 gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct gtctttaaag
    20461 atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt tccactggta
    20521 aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc agcatattgc
    20581 gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcggc cttgtctggt
    20641 tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca
    20701 tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg ctcggcatta
    20761 cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc cagattcatc
    20821 ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg
    20881 ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc gggagaaagc
    20941 gtgatgacgc gcggcgcggc gttgagccac agtggcgcaa gaaaagacag ggcgaccagc
    21001 gccctgaaca gtgacttagc catgtgcaag tttctgcacc agtgactcaa ccatcaggct
    21061 ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact gttgatcggc
    21121 cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc agacatgggc
    21181 gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc ggattttcgc
    21241 cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt
    21301 aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt cagctttaaa
    21361 gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga catccgcgtc
    21421 gtgataacgt gcttcgtccg agacaacgat atcgcccact ttcaacgttg gtgccaggcc
    21481 accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca gcaaagtggc
    21541 acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct cggttccatt
    21601 cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacgtt tttcgatttt
    21661 gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt tcatagattt
    21721 actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg aagcgtattt
    21781 ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac tggcatcgtc
    21841 gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc ttcgagagcg
    21901 atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag acgcaggttt
    21961 ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc
    22021 aggtggggcg ctacatcgcc aaagaaattt taagccgtct gaaagagctt aaattgctgg
    22081 aagcatacgg cctggatgaa ctgaccggac cttttgaaag cattgttgag atgtcatgcc
    22141 tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg ataaatgact
    22201 ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact gacgatcgct
    22261 gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag ctgcggcgca
    22321 agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt ctggtgcata
    22381 cattgatgcg gatgaatctc acctgggcac aggttggcgg tattttaaaa tatacccgtc
    22441 cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa aagccgggtt
    22501 attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat ttggcgcttt
    22561 acagtcgttt tccattaact tggattatgg aagctgccga cgacatctcc tattgtgtgg
    22621 cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt tatcatcatt
    22681 tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg gttgaaaatg
    22741 cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag ttttttatgt
    22801 atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga tttattgata
    22861 atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat gccagcgaat
    22921 gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt agccatccag
    22981 atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta gagatttatc
    23041 gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa gaacgggtga
    23101 aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat cggctggcct
    23161 atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta tgggaatatt
    23221 attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc tatgcgtggg
    23281 atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa gacgaacaat
    23341 aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa cgaatctgaa
    23401 gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa aaaaccacat
    23461 tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc tctgcaacgg
    23521 cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca ccgatgctcg
    23581 aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc gttaatacgc
    23641 cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc tgccaggaag
    23701 gttcaccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat ggcggcggcc
    23761 agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat aaaggctatg
    23821 tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag ctgggcgatg
    23881 gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc gcgctgattc
    23941 agatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat gcactgcgcg
    24001 tgggtgatta caccgtagcg attggtaacc cgtttggcct gggagagacg gtaacttccg
    24061 ggattgtctc tgcgctgggg cgtagcggcc tgaatgccga aaactacgaa aacttcatcc
    24121 agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac ctgaacggcg
    24181 aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc ggtatcggtt
    24241 ttgctatccc gagcaacatg gtgaaaaacc tgacctcgca gatggtggaa tacggccagg
    24301 tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg gcgaaagcga
    24361 tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat tcctccgctg
    24421 caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag ccaatcagca
    24481 gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa ctgaccctgg
    24541 gcttgctgcg cgacggtaag caggtcaacg tgaatctgga actgcagcag agcagccaga
    24601 atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg agcaacaaag
    24661 gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct gcgcagatcg
    24721 gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa aacatcgctg
    24781 aactgcgtaa agttcttgac agcaaaccgt ctgtactggc actgaacatt cagcgcggcg
    24841 acagctccat ctacctgtta atgcagtaat ctccctcaac cccttcctga aaacgggaag
    24901 gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat catcctttag
    24961 gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca taacggagga
    25021 cttatggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt accatgcgca
    25081 tcatcgatac caatatcaac gtaatggatg cccgtgggcg aattatcggc agcggcgatc
    25141 gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag ggacgagtcg
    25201 tcgatatcga tgacgcggtg gcacgtcatc tgcacggtgt gcggcaaggg attaatctac
    25261 cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc
    25321 tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt
    25381 tgatgcactt gttggctcag gatagccgtt tgcgggagga actggtgatg aacctgattc
    25441 aggcagagga gaatactccc gcacttactg aatgggcgca acggctgggg atcgatctca
    25501 atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggt gtggacagcg
    25561 caatggcgga gttacaacaa ctgcaaaacg cgctgactac gcccgagcgt aataatctgg
    25621 tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc
    25681 gctgggatgc agaagatcat cgtaagcgag ttgaacaact gattacccgc atgaaagagt
    25741 acggccagct gcgttttcgc gtttcactgg gcaactattt taccggtcct ggcagtattg
    25801 cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg atgccagaaa
    25861 gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg
    25921 actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gacaataacg
    25981 gcttgctgcg acgaacgctg gcggcgtggt ttcgtcacaa tgtgcaaccg ctggcaacgt
    26041 caaaggcgtt gtttattcat cgtaataccc tggagtatcg gcttaatcgt atatcggaac
    26101 tgaccgggct tgatttgggc aattttgatg acaggttgct gctgtatgtg gcgttacaac
    26161 tggatgaaga gcggtaggtt atgcgttaag gttgaggcgg cgctggctca ttcccctcag
    26221 cccgaagggg agaggggaat gcccgaatta accgtcaatt cgggattatt tacgcgttag
    26281 tttttccaga tcggcttcaa tctcgctgat cttattggtt acgactgact ccaggtgacg
    26341 taagtcgtcg aggatcttac gcttaagatc aacttcgctg cggtcacgct ggcagatttg
    26401 atcaagctca tcaatgatat accgtagatt cgggctgatt tcctggactt ctttataacc
    26461 ctgacccaca ccatcagcga cgaccgtttt acgctgacgc ggatatttaa acttaacgct
    26521 cttggcgaaa aactcgcctt tgtctttctg gaaatagatt ttcagaatat cgttgttggc
    26581 ttcctgccgg aggctgtaac gatcaatttc ttcaggattg gtaataccca gacttttcag
    26641 attgtcgtac atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt ttagcatttg
    26701 gcctggcccg cccggctcga ctgtgatcgc agaaagcctt tttcttccag acttctatca
    26761 tggcgtaaaa atcaaaaaat tacctgcttt attgtggtga taaaattcac gttctacacc
    26821 agttccagca tcgttacttc cgggcggcag ttcagacgca aaccatacaa actgcccacg
    26881 ccacgggttg tgtagatgtg tcgttcgcca aaggcattta atccggcgac gtaacgttta
    26941 tcttcgacag gggcaaaagg ttcaccaacc aacggtacgc gcagttgccc gccgtgagta
    27001 tggccgcaca gcatcagatc ccagggttca tcacgcatga cttctttgct gtcgggatta
    27061 tgcgccagca ccagtcttgg cagattggct tcgctggcgg gaggcggttt gcattgtccg
    27121 gcccataagt caccggtgcc taccagttcg aattgcctgt tcggcgtggc gatcaccgta
    27181 gcctggttaa acaacaccgt gatgcccgct gatttcaacg tctcgccaat taagtgattt
    27241 ttttctgtac caacagggcg atcgtggttg ccgaagcagg caaacgtcgg cgcacattcg
    27301 gcaagggggg agaggacatc actaaacgcc gaaaaattca gcggcatatc aaataatacg
    27361 taatcgccgc ccagtaatat caaatcgggc ttttgttcta tgccaagagc aatcgcgtca
    27421 gaaatcaggc ttaaaggaac aaaacgagag taatggagat cggccagaaa aagaattttg
    27481 aatggtgctg cgttgtcttt aaaaaaggcg aggcggtgac ggattaattc aaaccagcct
    27541 ggctcccagt aatgcatata accgaaacct gagctcgtgg ctatcgtcgc ggcagtagcc
    27601 tgcaacaatc ggcggcgtga aatcatcgct catccctgca aaaaaaatcg ggcagcgtcg
    27661 tgctgcccgt gtgcatactt ttagtcgatg gtacgcagca gttcgttaat gccgactttg
    27721 ccgcgagttt tcgcgtcaac tttcttaacg ataaccgcac agtagaggct gtatttgcca
    27781 tcttttgacg gcagattacc tgaaacaacc acagaccccg ccggaacgcg accgtagtgg
    27841 atttcgccgg tttcacggtc gtaaatacgg gtgctctgac caatgtatac gcccatggaa
    27901 atgacggaac cttcttcgac aatcacccct tcaaccactt cagagcgtgc gccaatgaag
    27961 cagttatctt caatgatggt tgggttagcc tgcagcggtt ccagcacgcc gccgatgccc
    28021 acgccaccgg aaaggtggac gtttttacca atctgcgcac aagaaccgac ggtcgcccag
    28081 gtatcaacca tggtgccttc atcaacatat gcgccgatgt tgacgtaaga cggcatcagc
    28141 acggtgttac gggcaataaa cgcaccctga cgtaccgccg ctggtggcac aacgcggaag
    28201 ccttctttct ggaaacgtgc ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag
    28261 tagcggcttt ctgccccttc gatcacctga ttatcattaa tacggaaaga gagcagcacc
    28321 gcttttttca accactgatg cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt
    28381 gcaccggaat ccagcagggc gatcacctga tttaccgctt cgcgggtaac ggtgtctgcg
    28441 ttggctggcg tgatctcggc acggcgttca aaagcggttt caataatgtt ctgtaactgc
    28501 tgcattgtta aactcttttc atatcagtaa acacatcacc ctttatcgtt tggattgagg
    28561 gcctctgtca accgctgatg cacttcctgc tgcaactcgt tattaagtgc acgccggtca
    28621 gcggtggcaa ttatgaataa atcttctact cgctcgccaa tggttgtaat tcgggcacca
    28681 tgaagcgaaa ttcccagatc ggcaaaaatt ttcccgactc tcgccagcag tcctggctgg
    28741 tcgagcgcga taagctcaag gaacgatttg cggtcggtat gggtcggcaa aaacgttact
    28801 tcggtttcaa cagtaaaatg gcgtaatttg gcgggttggc gacggggctg cggtggctgc
    28861 cagctacttt gcgtcagtac ttgctccaga ccaaaccgaa taacctcatg acgatctgcg
    28921 gacagcgggc tgccatcggg ttccagcacg ataaaggtat ccatcgccat accgtcgcga
    28981 gtggtgaaaa tttgtgcgtc gtgaacactt aaattgcggc ggtctaattc ggcacagacg
    29041 gcggcaaaca gataggggcg gtccgggctc cagataaaaa tctcggtgcc tccacgcgta
    29101 gcctgcgggc taagcaatac cagcggtttg cttaaatcat gctgtaataa atggcgggca
    29161 tgccaggcca gttgatttgg gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa
    29221 atttggtgca gcgcctcttc gtcgatgtta tccatgcgca gtagtgccag tgcctggagt
    29281 tggtgatgac gaacccgttc gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc
    29341 ttttcggtgg caaaatagag ttcgcgcaac agactttgct tccagctatt ccacagcgtt
    29401 tcgttggtgg cgcaaatgtc agccaccgtc aggcatacca gataacgaag tcggttttct
    29461 gtttgcactt cttcggcaaa ctgcttgatg acttccgggt cctgaatatc gcggcgttgg
    29521 gcggtcaccg acatcaacag gtgctggcga accagccagg caaccagctg tgtttcgcgc
    29581 gagttaagcc cgtggagttc ggcaaaatgc actacatcct gagcaccgag aatggagtgg
    29641 tcgccgccgc gtcctttggc gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt
    29701 gacggcaggc gcggccagac gtccacacac aacggatggc gctggcgcgt ttcttcactg
    29761 gcaaaactct ccagtttcag catcacgcgg atagtatgtt catccaccgt gtaggcgtgg
    29821 aacagatcaa actgcatctg cccgacgata tgcgaccatt gcggcatata cgcgccgagc
    29881 acgctatggc gatgcattgg caatagcccg cgccgcaccg ctccggggtg acgcagaatg
    29941 ctcaaaaaca gttttcgtgc ttccggaata ttacacagcg gttgttgcag atggcgacgg
    30001 gcatggcgta actggcgcag cgtggtggag taaatgccgg tgatcgcact gttgcgcacc
    30061 atggtgtaga acatacgcaa gatggcttcc ggctggcgca taaatagtgt ttcatcacgc
    30121 aggtcgatta gcgtaccgcg tagctgaaac tcatcgtcga gtggacgtgg tttttcgtcg
    30181 gcgggaaggg cgaggatggc ttcatcgaac agttgcagca gcatctggtt gagttcactg
    30241 acgcggcgtg taacgcggaa gtaatccttc atcatccgct cgaccggttc gttgccttca
    30301 ccactgtaat tcagacgctg agcgacgcta agttggcgat cgaataacag acgattatcg
    30361 taacggctga cgaccagatg cagggcaaag cgaatacgcc acagtatatg cagacattcg
    30421 tttaattccg cccgctccgc tgaggttaag aagccaaacc caaccatttc atccagcgat
    30481 gttgcgccaa aatgacggcg ggccacccat tgcagagtgt ggatatcgcg caagccgcca
    30541 gggctacttt tgatgtctgg ttcaagattg tagctggtgc cgtggtaacg ctgatggcgc
    30601 tggttctgtt cttcaacttt cgccgcgtag aacttgtcgg aaggccagaa tccttcgcta
    30661 aaaatatgtt tttgcagttc gaggaagagc gcaacatcgc caattaataa gcgggattcg
    30721 attaaattgg tggcgacggt taaatccgat aacccttcca gcatgcactc ttcaagcgtg
    30781 cgcacactat gaccgacttc cagctttaca tcccagagca gcgttaacag ctcgcccact
    30841 ttttgcgcct gatcgtccgg gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa
    30901 agtggatgca gctcgccacg accgtagcca ccgacggcga ccaatgccag gtcggcaatc
    30961 tggctgaatc ccgcttcaat ccataatcgt tgcaggagct ggtcgataaa ctcggtgcgc
    31021 gcctcaatca actgttctgc agagatccca ttgtcaaagg catcacccag ccaacgctgg
    31081 aaagtatcga tatgggcttt tatcccaccg acggttaatt catcacgggg ccagacgcat
    31141 ggattttgcg gttgaccggg cagggtgggg agagcggtgt ttgcgtactg ttctggaagg
    31201 gtattcattg tgcgccaccc ataagattaa attatcgcat taaaaaagcc ggcattcgcc
    31261 ggcttcatct tattcgtcgt gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt
    31321 cagaatttcg cagccgttat cagtcaccac aatagtatgc tcatattgtg cagacaagct
    31381 gcgatctttg gtttttaccg tccagccatc tttcatggtg cggatctctt ttttacccgc
    31441 gttgaccatt ggctcgatgg tgaacgtcat cccaggtttc agtacgacgt tggtttcacg
    31501 ggagtcatag tgcagcacct gcggttcttc atggaagccg cgaccaatac cgtgtccgca
    31561 atattcacga acgacggaga agccttctgc ttcgacaaat ttctgaatcg ccgcaccgat
    31621 ttcgcgcaga ttaatgcctg gtttgaccat gcgcagcgcc aggtacaggc tttcttgcgt
    31681 gatgcggcac agacgttcgc ccatgatggt cggcttaccg acgataaaca ttttcgaggt
    31741 atcgccgtgg aaaccatctt tgattacggt gacatcaatg ttaacgatat cgccatcttt
    31801 cagcagcttc gcatcgtccg ggataccgtg gcacaccact tcattaatag agatgcaaac
    31861 ggatttcgga tagccgtgat agccgaggca ggcagaaacc gtgtgttgtt cattaacaat
    31921 gtaatcatta cagatgcgat ccagctcgcc ggtgctgacg cccggtttaa catacggttc
    31981 gatcatctcc agcacttcgg cagccagtcg gccagcgacg cgcatttttt cgatatcttc
    32041 tggggtcttg attgagatag ccattaattc tgtccatcag cgtcggtgat accgacaata
    32101 tatatgtaag tgccgtcaat ggtatcacac ccgggcaaat tgagaatcat tctgaatttc
    32161 gccaaacgtg ccactgaacg ttttctataa tagaaaattc aacgtctgat gctgtacaca
    32221 gcgccaacaa ttattggtgt ccatgacgta tttgtggtat aaagcgcgcc ggacttccga
    32281 tccatttcgt atacacagac tggacggaag cgacaatctc actttgtgta acaacacaca
    32341 cgtatcggca catattccgg ggtgcccttt ggggtcggta atatgggata cgtggaggca
    32401 taaccccaac ttttatatag aggttttaat catggcaact gtttccatgc gcgacatgct
    32461 caaggctggt gttcacttcg gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt
    32521 catcttcggt gcgcgtaaca aagttcacat catcaacctt gagaaaactg taccgatgtt
    32581 caacgaagct ctggctgaac tgaacaagat tgcttctcgc aaaggtaaaa tccttttcgt
    32641 tggtactaaa cgcgctgcaa gcgaagcggt gaaagacgct gctctgagct gcgaccagtt
    32701 cttcgtgaac catcgctggc tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc
    32761 catcaaacgt ctgaaagacc tggaaactca gtctcaggac ggtactttcg aaaagctgac
    32821 caagaaagaa gcgctgatgc gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg
    32881 tatcaaagac atgggcggtc tgccggacgc tctgtttgta atcgatgctg accacgaaca
    32941 cattgctatc aaagaagcaa acaacctggg tattccggta tttgctatcg ttgataccaa
    33001 ctctgatccg gacggtgttg acttcgttat cccgggtaac gacgacgcaa tccgtgctgt
    33061 gaccctgtac ctgggcgctg ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc
    33121 ttcccaggcg gaagaaagct tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa
    33181 atagttcgag ttgcagaaag gcggcaagct cgagaattcc cgggagctta catcagtaag
    33241 tgaccgggat gagcgagcga agacaacgca tctgcggcgc gaaatatgaa gggggagagc
    33301 ccttatagac caggtagtac acgtttggtt agggggcctg catatggccc cctttttcac
    33361 ttttatatct gtgcggttta atgccgggca gatcacatct ccgaggattt tagaatggct
    33421 gaaattaccg catccctggt aaaagagctg cgtgagcgta ctggcgcagg catgatggat
    33481 tgcaaaaaag cactgactga agctaacggc gacatcgagc tggcaatcga aaacatgcgt
    33541 aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc
    33601 aaaaccaaaa tcgacggcaa ctacggcatc attctggaag ttaactgcca gactgacttc
    33661 gttgcaaaag acgctggttt ccaggcgttc gcagacaaag ttctggacgc agctgttgct
    33721 ggcaaaatca ctgacgttga agttctgaaa gcacagttcg aagaagaacg tgttgcgctg
    33781 gtagcgaaaa ttggtgagaa catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt
    33841 ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac
    33901 gaagagctgg ttaaacacat cgctatgcac gttgctgcaa gcaagccaga attcatcaaa
    33961 ccagaagacg tatccgctga agtggtagaa aaagaatacc aggtacagct ggatatcgcg
    34021 atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa
    34081 ttcaccggcg aagtttctct gaccggtcag ccgttcgtta tggaaccaag caaaactgtt
    34141 ggtcagctgc tgaaagagca taacgctgaa gtgactggct tcatccgctt cgaagtgggt
    34201 gaaggcatcg agaaagttga gactgacttt gcagcagaag ttgctgcgat gtccaagcag
    34261 tcttaattat caaaaaggag ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat
    34321 tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc
    34381 atttcaatcg ttgacagtct caggaaagaa acatggctac caatgcaaaa cccgtctata
    34441 aacgcattct gcttaagttg agtggcgaag ctctgcaggg cactgaaggc ttcggtattg
    34501 atgcaagcat actggatcgt atggctcagg aaatcaaaga actggttgaa ctgggtattc
    34561 aggttggtgt ggtgattggt gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg
    34621 gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc gaccgtaatg aacggcctgg
    34681 caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg tctgatgtcc gctattccat
    34741 tgaatggcgt gtgcgacagc tacagctggg cagaagctat cagcctgttg cgcaacaacc
    34801 gtgtggtgat cctctctgcc ggtacaggta acccgttctt taccaccgac tcagcagctt
    34861 gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg
    34921 tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat gtacgagcaa ctgacttaca
    34981 gcgaagtgct ggaaaaagag ctgaaagtca tggacctagc ggccttcacg ctggctcgtg
    35041 accataaatt accgattcgt gttttcaata tgaacaaacc gggtgcgctg cgccgtgtgg
    35101 taatgggtga aaaagaaggg actttaatca cggaataatt cccgtgatgg ataaataagg
    35161 gtaagattcc gcgtaagtat cgcgggggcg ttagtctggt tataaggcgt tattgttgca
    35221 ggcagtttgg tcacggccag cgcgcagcaa ccggagcgta caaaagtacg tgaggatggc
    35281 gagcactgct cggggccaaa atggcaaata aaatagccta ataatccaga cgattacccg
    35341 taatatgttt aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg
    35401 actgagacaa gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag
    35461 tacgcatgga caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc
    35521 gtgcttctcc cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc
    35581 tgcgtcagct ggcaagcgta acggtagaag attcccgtac actgaaaatc aacgtgtttg
    35641 atcgttcaat gtctccggcc gttgaaaaag cgattatggc gtccgatctt ggcctgaacc
    35701 cgaactctgc gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta
    35761 aagatctgac caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaacg
    35821 tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag
    35881 acgacgatcg ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa
    35941 ttgaagcggc gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac
    36001 aaaaacgccg ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt
    36061 ctcaactctg gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt
    36121 tgcagcacgc tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg
    36181 gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc
    36241 gtaatggacg atgaagcgag tgcgaaactt cttaaaacga tgctacagca acagggtagc
    36301 cgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata tggcagcgct tgaggatgtt
    36361 gatcaggtga tggcagccat tgttggcgct gctgggctgt tacctacgct tgctgcgatc
    36421 cgcgcgggta aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg
    36481 tttatggacg ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac
    36541 gccatttttc agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag
    36601 caaaatggcg tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca
    36661 ttgcgcgatt tggcaacaat gacgccggat caagcctgcc gtcatccgaa ctggtcgatg
    36721 gggcgtaaaa tttctgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa
    36781 gcgcgttggc tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca
    36841 gtgattcact caatggtgcg ctatcaggac ggcagtgttc tggcgcagct gggggaaccg
    36901 gatatgcgta cgccaattgc ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg
    36961 aagccgctcg atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgatcgt
    37021 tatccatgcc tgaaactggc gatggaggcg ttcgaacaag gtcaggcagc gacgacagca
    37081 ttgaatgccg caaacgaaat caccgttgct gcttttcttg cgcaacaaat ccgctttacg
    37141 gatatcgctg cgttgaattt atccgtactg gaaaaaatgg atatgcgcga accacaatgt
    37201 gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag tcgccagaaa agaggtgatg
    37261 cgtctcgcaa gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc
    37321 ttcagtgata tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg
    37381 ccgtggtttt acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc
    37441 tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc
    37501 cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa
    37561 agcaagggaa gattcgtgcc tttgggcata aagccggggc aaaatccgtc cgccgggctg
    37621 tctcttttgc ggccaacaac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa
    37681 actggaaccg accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg
    37741 atagcgaagt aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca
    37801 gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagccggga
    37861 atagcggtct gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg
    37921 gagtcaggca actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg
    37981 aagagatgct aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta
    38041 ggactggggg ggagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac
    38101 tttactttac agatgttctc tggcccgatt tcgatgaaca agactttgaa ggggcgttaa
    38161 atgcctttgc taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct
    38221 gatgggggtc gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg
    38281 tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc catcgtaacg ctggtggtct
    38341 gcatgctggc agcgtgggaa tggggacagc ttagcggttt taccactcgt tcgcagcgag
    38401 tatggttggc ggtgttatgc gggttattgt tggcgctgat gctttttctg ttgccggaat
    38461 atcaccgaaa tattcatcaa ccgctggttg aaatctcact ttgggcttcg ttgggttggt
    38521 ggattgtcgc gctattgctg gtgctgtttt acccaggttc cgcagcaatc tggcgtaact
    38581 ctaaaacatt gcgccttatt tttggcgtgc taaccattgt tcccttcttc tggggcatgc
    38641 tggcgttacg ggcctggcac tacgacgaga atcattacag tggcgcaata tggctgctct
    38701 atgtcatgat cctggtatgg ggcgctgact ctggcgcata tatgtttggc aaattgtttg
    38761 gtaaacataa gctggcaccg aaggtttctc cgggtaaaac ctggcaaggc tttataggtg
    38821 gactagcgac cgcagcggta atctcatggg gttatggcat gtgggcgaat ctcgacgtcg
    38881 ctcccgtcac cttactcatt tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc
    38941 tgaccgagag tatgtttaag cgtgaagcag gaattaagga cagcggtcat ttaattccag
    39001 gacacggtgg tattttagat cgtattgata gcctgacggc tgcggtaccg gtctttgctt
    39061 gcttgttgtt actggtattc aggacgcttt aacggaaggt aatatgctga gttttctctg
    39121 ggatttggct tcgttcatcg ttgcactggg tgtacttatc accgtgcatg aatttggtca
    39181 tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa
    39241 ggcgctctgg cggcgaactg ataagctcgg caccgaatat gttatcgccc tgatcccgct
    39301 gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg aactccgcca
    39361 ccatgccttc aataataaat ctgtcggcca acgagcggcg attattgccg caggtccggt
    39421 tgcaaacttc atttttgcta tctttgccta ctggctggtt tttattattg gtgtgcctgg
    39481 cgtacgtccg gtggttggtg aaatagcagc caattcgata gctgcggaag cacaaattgc
    39541 accaggtacg gaactaaaag ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg
    39601 tttgcagttg gtcgataaaa ttggcgatga aagcaccacc attacagtag cgccatttgg
    39661 cagcgaccaa cggcgggatg taaagctcga tttacgtcac tgggcgtttg agcctgataa
    39721 agaagatccg gtatcttcgc tggggattcg tcctcgtggg ccgcaaattg aacctgtact
    39781 ggaaaatgtg caaccaaact cggcggcaag caaggcaggt ttgcaagcag gcgacaggat
    39841 cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga
    39901 taacccgggt aaatccttag cgttagaaat cgaaaggcag ggaagtccct tgtctttgac
    39961 attaatcccg gagagtaaac cgggtaatgg taaagcgatt ggttttgtcg gtattgagcc
    40021 gaaagtcatt cctttgccag atgagtataa agttgtacgc cagtatgggc cgttcaacgc
    40081 catcgtcgaa gccacggaca aaacgtggca gctgatgaag ctgacggtca gtatgctggg
    40141 aaaattgatc accggtgatg tgaaactgaa caacctcagt gggccgatct ctatcgccaa
    40201 gggggctggg atgacagcgg aactcggggt agtttattac ctgccgtttc ttgcgcttat
    40261 tagcgtgaac ttagggataa ttaacctgtt tccgttgccc gtacttgacg gggggcatct
    40321 gctgttcctt gcgatcgaaa agatcaaggg cggaccggta tccgagcggg ttcaagactt
    40381 ttgttatcgc attggctcga ttctgctggt gctgttaatg gggcttgcac ttttcaatga
    40441 tttctctcgg ttatgagagt tagttaggaa gaacgcataa taacgatggc gatgaaaaag
    40501 ttgctcatag cgtcgctgct gtttagcagc gccaccgtat acggtgctga agggttcgta
    40561 gtgaaagata ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc cctcctcagt
    40621 atgccggtgc gcacaggcga cacggttaat gatgaagata tcagtaatac cattcgcgct
    40681 ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga tacccttctg
    40741 gttcaggtaa aagaacgtcc gaccattgcc agcattactt tctccggtaa caaatcggtg
    40801 aaagatgaca tgctgaagca aaacctcgag gcttctggtg tgcgtgtggg cgaatccctc
    40861 gatcgcacca ccattgccga tatcgagaaa ggtctggaag acttctacta cagcgtcggt
    40921 aaatatagcg ccagcgtaaa agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta
    40981 aaactggtgt tccaggaagg tgtgtcagct gaaatccagc aaattaacat tgttggtaac
    41041 catgctttca ccaccgacga actgatctct catttccaac tgcgtgacga agtgccgtgg
    41101 tggaacgtgg taggcgatcg taaataccag aaacagaaac tggcgggcga ccttgaaacc
    41161 ctgcgcagct actatctgga tcgcggttat gcccgtttca acatcgactc tacccaggtc
    41221 agtctgacgc cagataaaaa aggtatttac gtcacggtga acattaccga aggcgatcag
    41281 tacaagcttt ctggcgttga agtgagcggc aaccttgccg ggcactccgc tgaaattgag
    41341 cagctgacta agatcgagcc gggcgagcag tataacggca ccaaagtgac caagatggaa
    41401 gatgacatca aaaagcttct cggtcgctat ggttatgcct atccgcgcgt acagtcgatg
    41461 cctgaaatta acgatgccga caaaaccgtt aaattacgcg tgaacgttga tgcgggtaac
    41521 cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg
    41581 cgtcgcgaaa tgcgtcagat ggaaggtgca tggctgggaa gcgatctggt cgatcagggt
    41641 aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt
    41701 ccgggtagcc cggaccaggt agatgtcgtc tacaaggtaa aagagcgcaa caccggtagc
    41761 ttcaacttcg ggattggtta cggtactgaa agtggcgtga gcttccaggc tggtgtgcag
    41821 caggataact ggttaggtac aggttatgct gttggtatca acgggaccaa aaacgattac
    41881 cagacctatg ctgaactgtc ggtaaccaac ccgtacttca ctgtagatgg tgtaagcctc
    41941 ggtggtcgtc tcttctataa tgacttccag gcagatgacg ccgacctgtc cgactatacc
    42001 aacaagagtt atggtacaga cgtgacgttg ggcttcccga tcaacgaata taactcgctg
    42061 cgtgcaggtc tgggttatgt acataactcc ctgtccaaca tgcagcctca ggttgcgatg
    42121 tggcgttatc tgtactctat gggtgaacat ccgagcacct ctgatcagga taatagcttc
    42181 aaaacggacg acttcacgtt caactatggt tggacctata acaagcttga ccgtggttac
    42241 ttcccgacag atggttcacg tgtcaacctg accggtaaag tgaccattcc tggatcggat
    42301 aacgaatact acaaagtgac gttagacacg gcgacttatg tgccgatcga tgacgatcac
    42361 aaatgggttg ttctggggcg tacccgctgg ggttatggtg atggtttagg cggcaaagag
    42421 atgccgttct acgagaactt ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc
    42481 aatacaattg gtccgaaagc agtttacttc ccgcatcagg ccagtaatta tgatccggac
    42541 tatgattacg aatgtgcgac tcaggacggc gcgaaagacc tgtgtaaatc ggatgatgct
    42601 gtaggcggta acgccatggc ggttgccagc ctcgagttca tcaccccgac gccgtttatt
    42661 agcgataagt atgctaactc ggttcgtact tccttcttct gggatatggg taccgtttgg
    42721 gatacaaact gggattccag ccaatattct ggatatccgg actatagtga tccaagcaat
    42781 atccgtatgt ctgcgggtat cgcattacaa tggatgtccc cattggggcc gttggtgttc
    42841 tcctacgccc agccgttcaa aaagtacgat ggagacaagg cagaacagtt ccagtttaac
    42901 atcggtaaaa cctggtaagt gttctccaca aaggaatgta gtggtagtgt agcgatgact
    42961 ttaggcgatc aatataagat cgccgggcca cgcaaagaac tgcaccctcc ggtgcaaatg
    43021 ggatggtaag gagtttattg tgaaaaagtg gttattagcg gcaggtctcg gtttagcact
    43081 ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc aacatgggca gcctgttcca
    43141 gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa aatgagttca aaggccgtgc
    43201 cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa atgaaaaagc tgcagtccat
    43261 gaaagcgggc agcgatcgca ctaagctgga aaaagacgtg atggctcagc gccagacttt
    43321 tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg aagaacgcgg
    43381 caaactggtt actcgtatcc agactgctgt gaaatccgtt gccaacagcc aggatatcga
    43441 tctggttgtt gatgcaaacg ccgttgctta caacagcagc gatgtaaaag acatcactgc
    43501 cgacgtactg aaacaggtta aataagtaat gccttcaatt cgactggctg atttagcgca
    43561 gcagttggat gcagaactac acggtgatgg cgatatcgtc atcaccggcg ttgcgtccat
    43621 gcaatctgca caaacaggtc acattacgtt catggttaac ccaaaatacc gtgagcattt
    43681 aggcttgtgc caggcgtccg cggttgtcat gacccaggac gatcttcctt tcgcgaaaag
    43741 tgccgcgctg gtagtgaaga atccctacct gacttacgcg cgcatggcgc aaattttaga
    43801 taccacgccg cagcccgcgc agaacattgc acccagtgcg gtgatcgacg cgacggcgaa
    43861 gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg ttgaactggg
    43921 cgataacgtg attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa tcggtgcagg
    43981 ttcgcgtctc tgggcgaacg taaccattta ccatgagatc cagatcggtc agaattgcct
    44041 gatccagtcc ggaacagtgg taggcgcaga cggctttggt tatgccaacg atcgtggtaa
    44101 ctgggtgaag atcccacaga ttggtcgcgt aattattggc gatcgcgtgg agatcggtgc
    44161 ctgcacaacc atcgatcgcg gcgcgctgga tgacactatt attggcaatg gcgtgatcat
    44221 tgataaccag tgccagattg cacataacgt cgtgattggc gacaatacgg cggttgccgg
    44281 tggcgtcatt atggcgggca gcctgaaaat tggtcgttac tgcatgatcg gcggagccag
    44341 cgtaatcaac gggcatatgg aaatatgcga caaagtgacg gttacgggca tgggtatggt
    44401 gatgcgtccc atcactgaac caggcgtcta ttcctcaggc attccgctgc aacccaacaa
    44461 agtctggcgc aaaaccgctg cactggtgat gaacattgat gacatgagca agcgtctgaa
    44521 atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt cgccaaactt
    44581 tacggcctgt ctcattctta cgattgcggc aggccgtgtt attattgtcg tttcttatat
    44641 tttgacagga agagtatctt gactactaac actcatactc tgcagattga agagatttta
    44701 gaacttctgc cgcaccgttt cccgttctta ctggtggatc gcgtgctgga ttttgaagaa
    44761 ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt ccagggccat
    44821 ttccctggaa aaccgatttt cccgggtgtg ctgattctgg aagcaatggc acaggcaaca
    44881 ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta ctacttcgct
    44941 ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat gatcatggaa
    45001 gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc tctggtcgat
    45061 ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc ctgatacgtg
    45121 attgataaat ccgcctttgt gcatccaacc gccattgtgg aagagggcgc gtcaattggc
    45181 gcgaacgcac acattggtcc tttttgtatc gttggacccc atgtcgaaat tggtgagggt
    45241 accgtactga aatctcacgt tgtcgtgaat ggtcatacta aaattggccg cgataatgag
    45301 atttatcagt tcgcctccat cggcgaagtt aaccaggatc tgaaatatgc tggcgaaccg
    45361 acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat tcatcgtggc
    45421 acagtccagg gcggtggatt gacgaaggtg ggcagcgaca acttactgat gatcaacgcg
    45481 cacattgcgc acgattgtac ggtaggtaac cgctgtattc tcgccaacaa cgcaacgctg
    45541 gcgggtcacg tatcggttga cgacttcgcg atcatcggcg gcatgaccgc agtccatcag
    45601 ttctgcatca ttggtgcgca cgtgatggtt ggcggctgct ccggtgtggc gcaggacgtc
    45661 cctccttatg tcattgcgca gggtaaccac gcaacgccgt tcggtgtcaa tatcgaaggg
    45721 ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta tccgcaatgc gtataagctg
    45781 atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga actggcggaa
    45841 acatatccgg aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg cggtctgatt
    45901 cgttaatgac tgaacagcgt ccattaacga ttgccctggt cgccggagaa acctccggcg
    45961 atatcctggg ggccggttta atccgcgctc tgaaagaacg tgtgcccaac gcccgctttg
    46021 ttggtgttgc cgggccacga atgcaggctg aaggctgcga agcctggtac gaaatggaag
    46081 aactggcggt gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta ctgcatattc
    46141 gtgccgatct gacaaagcgt tttggcgaac tgaagccaga tatttttgtt ggtattgatg
    46201 cgcctgactt caatattact cttgaaggta acctcaaaaa gcagggtatc aaaaccattc
    46261 attacgtcag tccatccgtc tgggcgtggc gacagaaacg cgttttcaaa ataggcagag
    46321 ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc gttttatgac aaatacaatg
    46381 taccgtgccg ctttatcggt cataccatgg ctgatgccat gccgttagat ccagataaaa
    46441 atgccgcccg tgatgtgctg gggatccctc acgatgccca ctgcctggcg ttgctaccag
    46501 ggagccgtgg tgcagaagtt gaaatgctta gtgccgattt cctgaaaacg gcccagcttt
    46561 tgcgccagac atatccggat ctcgaaatcg tggtgccact ggtgaatgcc aaacgccgcg
    46621 agcagtttga acgcatcaaa gctgaagtcg cgccagacct ttcagttcat ttgctggatg
    46681 ggatgggccg tgaggcgatg gtcgccagcg atgcggcgct actggcgtcg ggtacggcag
    46741 ccctggagtg tatgctggcg aaatgcccga tggtggtggg atatcgcatg aagcctttta
    46801 ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt ctcgctgcca aatctgctgg
    46861 cgggtagaga gttagtcaaa gaattattgc aggaagagtg tgagccgcaa aaactggctg
    46921 cggcgctgtt accgctgttg gcgaacggga aaaccagcca cgcgatgcac gataccttcc
    46981 gtgaactgca tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa gccgttctgg
    47041 agttagcaca atgatcgaat ttgtttatcc gcacacgcag ctggttgcgg gtgtggatga
    47101 agtcggacgc gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc ttgacccggc
    47161 gcgcccgatt gccgggctga atgattccaa aaagctgagc gaaaaacgcc gtctggcgct
    47221 ctatgaagag atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg aaccccacga
    47281 aatcgacgag ctgaacattc ttcatgcgac catgctggcg atgcagcgtg ccgtcgctgg
    47341 gctgcatatt gcgccggaat atgtgttgat tgatggtaac cgctgcccga aattaccgat
    47401 gcctgcgatg gctgtggtga aaggcgatag ccgcgtaccg gaaatcagtg ccgcgtctat
    47461 cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg gatattgttt tcccgcaata
    47521 tggttttgcc caacacaaag ggtacccaac cgcttttcat ctggaaaaac tggctgaaca
    47581 cggcgcgacc gaacaccatc ggcgcagctt tgggcctgtc aaacgcgcac tgggacttgc
    47641 gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg aaccacgttt
    47701 cgtacacctg cgggtgcaca gcgactactc gatgatcgat ggcctggcca aaaccgcacc
    47761 gttggtaaaa aaggcggcgg cgttgggtat gccagcactg gcgatcaccg atttcaccaa
    47821 cctttgtggt ctggtgaagt tctacggagc gggacatggc gcagggatta agcctatcgt
    47881 cggggcagat tttaacgtcc agtgcgacct gctgggtgat gagttaaccc acctgacggt
    47941 actggcggcg aacaataccg gctatcagaa tctgacgttg ctgatctcaa aagcgtatca
    48001 gcgcgggtac ggtgccgccg ggccgatcat cgatcgcgac tggcttatcg aattaaacga
    48061 agggttgatc cttctttccg gcggacgcat gggcgacgtc ggacgcagtc ttttgcgtgg
    48121 taacagagcg ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta
    48181 ttttctcgag ctgatccgca ccggcaggcc ggatgaagaa agctatctgc acgcggcggt
    48241 ggaactggcg gaagcgcgcg gtttgcccgt cgtggcgacc aacgacgtgc gctttatcga
    48301 cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc cacgacggct ttaccctcga
    48361 cgatcctaaa cgcccgcgta actattcgcc gcagcaatat atgcgtagcg aagaggagat
    48421 gtgtgagctg tttgccgaca tccccgaagc ccttgccaac accgttgaga tcgccaaacg
    48481 ctgtaacgta accgtgcgtc ttggtgaata cttcctgccg cagttcccga ccggggacat
    48541 gagcaccgaa gattatctgg tcaagcgtgc aaaagagggc ctggaagagc gtctggcctt
    48601 tttattccct gacgaggaag aacgtgttaa gcgccgcccg gaatatgacg aacgtctgga
    48661 gactgaactt caggttatca accagatggg cttcccgggc tacttcctca tcgttatgga
    48721 atttatccag tggtcgaaag ataacggcgt accggtaggg ccaggccgtg gctccggtgc
    48781 gggttcactg gtggcctacg cgctgaaaat caccgacctc gatccgctgg aatttgacct
    48841 gctgttcgaa cgtttcctta acccggaacg tgtctccatg cctgacttcg acgttgactt
    48901 ctgtatggag aaacgcgatc aggttatcga gcacgtagcg gacatgtacg gtcgtgatgc
    48961 ggtatcgcag atcatcacct tcggtacaat ggcggcgaaa gcggtgatcc gcgacgtagg
    49021 ccgcgtgctg gggcatccgt acggctttgt cgatcgtatc tcgaaactga tcccgcccga
    49081 tccggggatg acgctggcga aagcgtttga agccgagccg cagctgccgg aaatctacga
    49141 agcggatgaa gaagttaagg cgctgatcga catggcgcgc aaactggaag gggtcacccg
    49201 taacgccggt aagcacgccg gtggggtggt tatcgcgccg accaaaatta ccgattttgc
    49261 gccgctttac tgcgatgaag agggcaaaca tccggtcacc cagtttgata aaagcgacgt
    49321 tgaatacgcc ggactggtga agttcgactt ccttggtttg cgtacgctca ccatcatcaa
    49381 ctgggcgctg gagatgatca acaagcggcg ggcgaagaat ggcgagccgc cgctggatat
    49441 cgctgcgatc ccgctggatg ataagaaaag cttcgacatg ctgcaacgct cggaaaccac
    49501 ggcggtattc cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc tacaacctga
    49561 ctgcttcgaa gatatgatcg ccctagtggc actgttccgc cccggtccgt tgcaatcagg
    49621 gatggtggat aactttatcg accgtaaaca tggtcgtgaa gagatctcct atccggacgt
    49681 acagtggcag catgaaagcc tgaaaccggt actggagcca acctacggca ttatcctgta
    49741 tcaggaacag gtcatgcaga ttgcgcaggt gctttctggt tataccctcg gtggcgcgga
    49801 tatgctgcgt cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc aacgttctgt
    49861 atttgctgaa ggtgcagaaa agaacggaat caacgctgaa ctggcgatga aaatcttcga
    49921 cctggtggag aaattcgctg gttacggatt taacaaatcg cactctgcgg cctatgcttt
    49981 ggtgtcatat caaacgttat ggctgaaagc acactatcct gcggagttta tggcggcggt
    50041 aatgaccgcc gatatggaca acaccgagaa ggtggtgggt ctggtggatg agtgctggcg
    50101 gatggggctg aaaatcctgc caccagatat aaactccggt ctttaccatt tccacgtcaa
    50161 cgacgacggc gaaatcgtgt atggtattgg cgcgatcaaa ggggtcggtg aaggtccgat
    50221 tgaggccatc atcgaagccc gtaataaagg cggctacttc cgcgaactgt ttgatctctg
    50281 cgcccgtacc gacaccaaaa agttgaaccg tcgcgtgctg gaaaaactga tcatgtccgg
    50341 ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt
    50401 aaaagcggca gatcaacacg cgaaagcgga agctatcggt caggccgata tgttcggcgt
    50461 gctggccgaa gagccggaac aaattgaaca atcctacgcc agctgccaac cgtggccgga
    50521 gcaggtggta ttagatgggg aacgtgaaac gttaggcctg tacctgaccg gacaccctat
    50581 caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga aagacatgca
    50641 cccgacagaa cgtggtaaag tcatcacggc tgcggggctc gttgttgccg cgcgggttat
    50701 ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct
    50761 ggaagtgatg ttgtttactg acgccctgga taaataccag caattgctgg aaaaagaccg
    50821 catacttatc gtcagcggac aggtcagctt tgatgacttc agcggtgggc ttaaaatgac
    50881 cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat
    50941 ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac cgactccgtc agtctctgga
    51001 accccaccgc tctgggacaa ttccagtaca tctctactat cagagggcgg atgcacgcgc
    51061 gcggttgcgt tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat taaacgatct
    51121 ccgtggcctc attggttcgg agcaggtgga actggagttt gactaataca ggaatactat
    51181 gagtctgaat ttccttgatt ttgaacagcc gattgcagag ctggaagcga aaatcgattc
    51241 tctgactgcg gttagccgtc aggatgagaa actggatatt aacatcgatg aagaagtgca
    51301 tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc ttcgccgatc ttggtgcatg
    51361 gcagattgcg caactggcac gccatccaca gcgtccttat accctggatt acgttcgcct
    51421 ggcatttgat gaatttgacg aactggctgg cgaccgcgcg tatgcagacg ataaagctat
    51481 cgtcggtggt atcgcccgtc ttgacggtcg tccggtgatg atcattggtc atcaaaaagg
    51541 tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg
    51601 caaagcactg cgtctgatgc aaatggctga acgctttaag atgcctatca tcacctttat
    51661 cgacaccccg ggggcttatc cgggcgtggg cgcagaagaa cgtggtcagt ctgaagccat
    51721 tgcacgcaac ctgcgtgaaa tgtctcgcct cggtgtaccg gtagtttgta cggttatcgg
    51781 tgaaggtggt tctggcggcg cgctggcgat tggcgtgggc gataaagtga atatgctgca
    51841 atacagcacc tattccgtta tctcgccgga aggttgtgcg tccattctgt ggaagagcgc
    51901 tgacaaagcg ccgctggcag ctgaagcgat gggtatcatc gctccgcgtc tgaaagaact
    51961 aaaactgatc gactccatca tcccggaacc gctgggtggt gctcaccgta acccggaagc
    52021 gatggcggca tcgttgaaag cgcaactgct ggcggatctg gccgatctcg acgtgttaag
    52081 cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt acgcgtaatt
    52141 cgcaaaagtt ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac ggtttgagca
    52201 ggctatgatt aaggaaggat tttccaggag gaacacatga acatcattgc cattatggga
    52261 ccgcatggcg tcttttataa agatgagccc atcaaagaac tggagtcggc gctggtggcg
    52321 caaggctttc agattatctg gccacaaaac agcgttgatt tgctgaaatt tatcgaacat
    52381 aaccctcgaa tttgcggcgt gatttttgac tgggatgagt acagtctcga tttatgcagc
    52441 gatatcaatc aacttaatga atatctcccg ctttatgcct tcatcaacac ccactcgacg
    52501 atggatgtca gcgtgcagga tatgcggatg gcgctctggt tttttgaata tgcgctgggg
    52561 caggcggaag atatcgccat tcgtatgcgt cagtacaccg acgaatatct tgataacatt
    52621 acaccgccgt tcacgaaagc cttgtttacc tacgtcaaag agcggaagta caccttttgt
    52681 acgccggggc atatgggcgg caccgcatat caaaaaagcc cggttggctg cctgttttat
    52741 gattttttcg gcgggaatac cctcaaggct gatgtctcta tttcggtcac cgagcttggt
    52801 tcgttgctcg accacaccgg gccacacctg gaagcggaag agtacatcgc gcggactttt
    52861 ggcgcggaac agagttatat cgttaccaac ggaacatcga cgtcgaacaa aattgtgggt
    52921 atgtacgccg cgccatccgg cagtacgctg ttgatcgacc gcaattgtca taaatcgctg
    52981 gcgcatctgt tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg
    53041 ttggggattc ttggtgggat cccgcgccgt gaatttactc gcgacagcat cgaagagaaa
    53101 gtcgctgcca ccacgcaggc acaatggccg gttcatgcgg tgattaccaa ctccacctat
    53161 gatggcttgc tctacaacac cgactggatc aaacagacgc tggatgtccc gtcgatccac
    53221 ttcgattccg cctgggtgcc gtacacccat tttcatccga tctaccaggg gaaaagtggt
    53281 atgagcggcg agcgtgttgc gggaaaagtg atcttcgaaa cgcaatcgac ccacaaaatg
    53341 ctggcggcgt tatcgcaggc ttcgctgatc cacattaaag gcgagtatga cgaagaggcg
    53401 tttaacgaag cctttatgat gcataccacc acctcgccca gttatcccat tgttgcttcg
    53461 gttgagacgg cggcggcgat gctgcgtggt aatccgggca aacggctgat taaccgttcg
    53521 gtggagcggg cgctgcattt tcgcaaagag gtccagcggt tgcgggaaga gtctgacggt
    53581 tggtttttcg atatctggca accgccgcag gtggatgaag ccgaatgctg gcccgttgcg
    53641 ctaggcgaac agtggcacgg ctttaacgat gcggatgccg atcatatgtt tctcgatccg
    53701 gttaaagtca ctattttgac accggggatg gacgaacagg gcaatatgag cgaggagggg
    53761 atcccggcgg cgctggtggc aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc
    53821 ggcccttata acctgctgtt tctctttagt attggtatcg ataaaaccaa agcaatggga
    53881 ttattgcgtg ggttgacgga atttaaacgc tcttacgatc tcaacctgcg gatcaaaaat
    53941 atgctacccg atctctatgc tgaagatccc gatttctacc gcaatatgcg tattcaggat
    54001 ctggcgcaag ggatccataa gctgattcgt aaacacgatc ttcccggttt gatgttgcgg
    54061 gcattcgata ctttgccgga aatgatcatg acgccacatc aggcatggca gagacagatt
    54121 aaaggtgaag tagaaaccat tgcgctggaa caactggtcg gtagagtatc ggcaaatatg
    54181 atcctgcctt atccaccggg cgtaccgctg ttgatgccag gagaaatgct gaccaaagag
    54241 agccgcacgg tactcgattt tctactgatg ctttgttccg tagggcaaca ttaccccggt
    54301 tttgaaacgg atattcacgg cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc
    54361 ctaaaaatgg cgggataact tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa
    54421 ataaaggaga cgttatgctg ggtttaaaac aggttcacca tattgcgatt attgcgacgg
    54481 attatgcggt gagcaaagct ttctactgcg atattcttgg tttcacgctg caaagcgaag
    54541 tctatcgcga agcgcgcgac tcgtggaaag gggatttggc gcttaatggg caatatgtga
    54601 ttgagctttt ctcttttccg ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc
    54661 tgcgtcatct ggcttttagc gttgatgaca tcgatgcggc agtggcgcac cttgaaagcc
    54721 ataacgtgaa gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc ttcaccttct
    54781 ttaacgatcc ggacgggctg ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc
    54841 atttgcccgg taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat gacactcacg
    54901 ctcaatagac aacttctcac ctcacgccag attctggtgg cctttagcgg cgggcttgac
    54961 tccaccgttc tgctgcatca gttggtgcag tggcggacgg aaaatccggg cgtcactctg
    55021 cgcgctattc atgtgcatca cggtttaagt gccaatgccg atgcctgggt tacgcattgc
    55081 gaaaacgtct gccaacagtg gcaggtgccg ctggtggtcg aacgcgtgca acttgcgcaa
    55141 gaaggactgg gcattgaggc ccaggcgcgg caggcgcgtt atcaggcatt tgcccgcacc
    55201 ttgttgcccg gtgaagtgct ggtcaccgcg caacatctcg acgatcaatg tgaaactttt
    55261 ctgctggcgc taaaacgcgg tagcggccct gccgggcttt cggctatggc ggaagtttcg
    55321 gagtttgccg gaacacggct tatccgcccg ttgctcgccc gcacgcgggg agaactggag
    55381 cagtgggcat tggcgcatgg tttacgctgg attgaagacg aaagtaatca ggacgactca
    55441 tacgatcgta actttctgcg cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat
    55501 tttgcggaag caacggcccg cagcgccgca ctttgtgctg aacaagagag tctgttggat
    55561 gaactgctgg cggatgattt agcacactgc caaacgtcgc aggggacgct gcagattgcg
    55621 ccaatgctgg cgatgagtga tgcccgccgc gcggcgatta tccgtcgctg gctggcaggg
    55681 cagaatgcac caatgccttc ccgcgacgcg ctggtgcgga tctggcagga agtggcgctg
    55741 gcgcgggaag atgcctcccc ctgtttacgc ttgggcgcgt ttgaaatccg ccgctatcag
    55801 tcgcaactgt ggtggattaa atccgtcacc gggcaaagtg aaaccattgt gccgtggcag
    55861 acgtggctac aaccgctgga attaccggcg gggctgggaa gcgtacagct tactgcggga
    55921 ggcgatattc gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa agcggcagga
    55981 ttgctgcata ttgtcgggcg caacggcgga cgtaagctga agaaaatctg gcaagagtta
    56041 ggcgtgccgc cgtggctacg tgacaccacg ccactgctgt tttatggcga aacgctgatt
    56101 gcggcggcag gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa tggcgtaagt
    56161 tttgtctggc agaaaacgct tagttaagtg aaagccggat aagacgcatc aaacgtcgca
    56221 tccggcgaaa gtcaatcagg actcgctcac caccaccgta ccgatttccg ggtggctaaa
    56281 gctggtaatt ttatccagac gtagctcacg ggtttcgcca gcggcctcga cgaccagata
    56341 ctccacattt ttgcgggaga ctaaatcgct ggcttttgcc tgcaattttt cgccatcttt
    56401 cagctcaagt gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc
    56461 acaattgatt ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg
    56521 cagcgtatgc tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat
    56581 tcagcacttt caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga
    56641 tttccgcgta ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca
    56701 ctctgactta accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt
    56761 gactatacca cactcatgtc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat
    56821 ggcctcctga ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac
    56881 ggttaatgtg gccgatctcg accgcaacca gtttctcgat gcctctctga cgctggcgcg
    56941 ccacccttca gaaacccagg agcgtatgat gctgcgcttg ctggcgtggc tgaaatatgc
    57001 tgatgaacgt ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct
    57061 gcgtaacgat cacctgggca ttgatttgtg gattgagctg gggctgccgg atgagcggcg
    57121 gattaagaaa gcctgcaccc aggccgcaga agtggcgctg tttacctata atagtcgggc
    57181 ggcgcaaatc tggtggcagc aaaatcagag caaatgtgtg cagtttgcca atctttccgt
    57241 ctggtatctg gacgatgaac aactggcgaa agtaagcgcc tttgccgatc gtaccatgac
    57301 gctgcaggca acgattcagg atggcgtgat ctggttatcg gacgataaga ataatctgga
    57361 agttaactta accgcctggc aacaaccttc atgattgtga tttcccgaca tgttgctatt
    57421 cccgatggtg agcttgagat caccgccatt cgtgcgcagg gcgcgggcgg gcagcatgtt
    57481 aataagacct caacggccat tcatctgcgt tttgacattc gggcgtccag cctgccagag
    57541 tattacaaag agcgtctact cgccgccagc catcatttga tcagcagtga tggcgtgatt
    57601 gtcattaagg cacaggaata ccgcagtcag gaactgaacc gcgaagccgc gctagcccgg
    57661 ctggtggcag tgattaaaga tttaacaaca gaacaaaaag cccgacgacc cacgcggccc
    57721 acccgtgcat cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg
    57781 atgcgcggca aagtacgcag cggtcgggaa taaaaagaag gaatagatgg tgaaaaaagc
    57841 gatagtgaca gcgatggctg taatcagcct ctttactctg atgggatgta ataaccgggc
    57901 cgaagtcgat acgctttctc cagcacaggc tgccgaactg aaaccgatgc cgcaaagttg
    57961 gcgcggcgtg ctgccgtgtg ctgattgcga aggaatcgaa acctctctgt tcctcgaaaa
    58021 agacggaaca tgggtgatga atgagcgtta tctcggtgct cgtgaagaac cttcctcctt
    58081 cgcttcctac ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg
    58141 tgaaaagtca tattatcggg cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa
    58201 tccgattgaa tcgcagttca actatacgct ggaaccggca caatctagtt tacctatgac
    58261 gccgatgacc ctgcgaggca tgtattttta tatggctgat gcggcgacct tcactgattg
    58321 cgcgaccgga aaacgtttca tggtagcgaa taacgcagag ctggagcgta gctacctggc
    58381 tgcgcgcggt cacagtgaaa aaccggtgtt actgtcagta gaaggtcact ttacgcttga
    58441 ggctaatccg gatactggtg cgccaaccaa agtattagcg cccgatacgg cgggtaaatt
    58501 ttacccgaat aaggattgca gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac
    58561 gcaa
//
LOCUS       DAZKGS010000010        55749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKGS010000010 DAZKGS010000000
VERSION     DAZKGS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..729
                     /gene="pspA"
                     /locus_tag="ACHOQY_000641"
     CDS             61..729
                     /gene="pspA"
                     /locus_tag="ACHOQY_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9676792.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            783..1007
                     /gene="pspB"
                     /locus_tag="ACHOQY_000642"
     CDS             783..1007
                     /gene="pspB"
                     /locus_tag="ACHOQY_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9676793.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            1007..1366
                     /gene="pspC"
                     /locus_tag="ACHOQY_000643"
     CDS             1007..1366
                     /gene="pspC"
                     /locus_tag="ACHOQY_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9676794.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            1375..1596
                     /gene="pspD"
                     /locus_tag="ACHOQY_000644"
     CDS             1375..1596
                     /gene="pspD"
                     /locus_tag="ACHOQY_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9676795.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            1671..1985
                     /gene="pspE"
                     /locus_tag="ACHOQY_000645"
     CDS             1671..1985
                     /gene="pspE"
                     /locus_tag="ACHOQY_000645"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9676796.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            2197..3876
                     /gene="ycjM"
                     /locus_tag="ACHOQY_000646"
                     /pseudo
     CDS             2197..3876
                     /gene="ycjM"
                     /locus_tag="ACHOQY_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            3890..5182
                     /gene="ycjN"
                     /locus_tag="ACHOQY_000647"
     CDS             3890..5182
                     /gene="ycjN"
                     /locus_tag="ACHOQY_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9676797.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            5203..6084
                     /gene="ycjO"
                     /locus_tag="ACHOQY_000648"
     CDS             5203..6084
                     /gene="ycjO"
                     /locus_tag="ACHOQY_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9676798.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            6071..6913
                     /gene="ycjP"
                     /locus_tag="ACHOQY_000649"
     CDS             6071..6913
                     /gene="ycjP"
                     /locus_tag="ACHOQY_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9676799.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            6944..7996
                     /gene="ycjQ"
                     /locus_tag="ACHOQY_000650"
     CDS             6944..7996
                     /gene="ycjQ"
                     /locus_tag="ACHOQY_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9676800.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            8014..8802
                     /gene="ycjR"
                     /locus_tag="ACHOQY_000651"
     CDS             8014..8802
                     /gene="ycjR"
                     /locus_tag="ACHOQY_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9676801.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            8812..9867
                     /gene="ycjS"
                     /locus_tag="ACHOQY_000652"
     CDS             8812..9867
                     /gene="ycjS"
                     /locus_tag="ACHOQY_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9676802.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            9864..12131
                     /gene="ycjT"
                     /locus_tag="ACHOQY_000653"
     CDS             9864..12131
                     /gene="ycjT"
                     /locus_tag="ACHOQY_000653"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP9676803.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            12128..12787
                     /gene="pgmB"
                     /locus_tag="ACHOQY_000654"
     CDS             12128..12787
                     /gene="pgmB"
                     /locus_tag="ACHOQY_000654"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9676804.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            12801..13883
                     /locus_tag="ACHOQY_000655"
     CDS             12801..13883
                     /locus_tag="ACHOQY_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9676805.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            13928..14833
                     /gene="ompG"
                     /locus_tag="ACHOQY_000656"
     CDS             13928..14833
                     /gene="ompG"
                     /locus_tag="ACHOQY_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9676806.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(14944..15942)
                     /gene="ycjW"
                     /locus_tag="ACHOQY_000657"
     CDS             complement(14944..15942)
                     /gene="ycjW"
                     /locus_tag="ACHOQY_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9676807.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            16097..17494
                     /gene="ycjX"
                     /locus_tag="ACHOQY_000658"
     CDS             16097..17494
                     /gene="ycjX"
                     /locus_tag="ACHOQY_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9676808.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            17491..18552
                     /gene="ycjF"
                     /locus_tag="ACHOQY_000659"
     CDS             17491..18552
                     /gene="ycjF"
                     /locus_tag="ACHOQY_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9676809.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            18700..20241
                     /gene="tyrR"
                     /locus_tag="ACHOQY_000660"
     CDS             18700..20241
                     /gene="tyrR"
                     /locus_tag="ACHOQY_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9676810.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(20285..20791)
                     /gene="tpx"
                     /locus_tag="ACHOQY_000661"
     CDS             complement(20285..20791)
                     /gene="tpx"
                     /locus_tag="ACHOQY_000661"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9676811.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            20910..21875
                     /gene="ycjG"
                     /locus_tag="ACHOQY_000662"
     CDS             20910..21875
                     /gene="ycjG"
                     /locus_tag="ACHOQY_000662"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9676812.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(21850..22578)
                     /gene="mpaA"
                     /locus_tag="ACHOQY_000663"
     CDS             complement(21850..22578)
                     /gene="mpaA"
                     /locus_tag="ACHOQY_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9676813.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(22887..23525)
                     /locus_tag="ACHOQY_000664"
     CDS             complement(22887..23525)
                     /locus_tag="ACHOQY_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9676814.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            complement(23543..24475)
                     /locus_tag="ACHOQY_000665"
     CDS             complement(23543..24475)
                     /locus_tag="ACHOQY_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9676815.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            complement(24475..25395)
                     /locus_tag="ACHOQY_000666"
     CDS             complement(24475..25395)
                     /locus_tag="ACHOQY_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9676816.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            25533..26432
                     /gene="pgrR"
                     /locus_tag="ACHOQY_000667"
     CDS             25533..26432
                     /gene="pgrR"
                     /locus_tag="ACHOQY_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9676817.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            26769..28382
                     /gene="mppA"
                     /locus_tag="ACHOQY_000668"
     CDS             26769..28382
                     /gene="mppA"
                     /locus_tag="ACHOQY_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9676818.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(28433..29464)
                     /gene="ynaI"
                     /locus_tag="ACHOQY_000669"
     CDS             complement(28433..29464)
                     /gene="ynaI"
                     /locus_tag="ACHOQY_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9676819.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            29708..29965
                     /gene="ynaJ"
                     /locus_tag="ACHOQY_000670"
     CDS             29708..29965
                     /gene="ynaJ"
                     /locus_tag="ACHOQY_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9676820.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(30015..30965)
                     /gene="uspE"
                     /locus_tag="ACHOQY_000671"
     CDS             complement(30015..30965)
                     /gene="uspE"
                     /locus_tag="ACHOQY_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9676821.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(31117..31869)
                     /gene="fnr"
                     /locus_tag="ACHOQY_000672"
     CDS             complement(31117..31869)
                     /gene="fnr"
                     /locus_tag="ACHOQY_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9676822.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(32064..32579)
                     /gene="ogt"
                     /locus_tag="ACHOQY_000673"
     CDS             complement(32064..32579)
                     /gene="ogt"
                     /locus_tag="ACHOQY_000673"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9676823.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(32590..34116)
                     /gene="abgT"
                     /locus_tag="ACHOQY_000674"
     CDS             complement(32590..34116)
                     /gene="abgT"
                     /locus_tag="ACHOQY_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP9676824.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(34153..35598)
                     /gene="abgB"
                     /locus_tag="ACHOQY_000675"
     CDS             complement(34153..35598)
                     /gene="abgB"
                     /locus_tag="ACHOQY_000675"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP9676825.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(35598..36908)
                     /gene="abgA"
                     /locus_tag="ACHOQY_000676"
     CDS             complement(35598..36908)
                     /gene="abgA"
                     /locus_tag="ACHOQY_000676"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP9676826.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            37084..37992
                     /gene="abgR"
                     /locus_tag="ACHOQY_000677"
     CDS             37084..37992
                     /gene="abgR"
                     /locus_tag="ACHOQY_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9676827.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            38322..38885
                     /gene="smrA"
                     /locus_tag="ACHOQY_000678"
     CDS             38322..38885
                     /gene="smrA"
                     /locus_tag="ACHOQY_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9676828.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(38906..40138)
                     /gene="dgcM"
                     /locus_tag="ACHOQY_000679"
     CDS             complement(38906..40138)
                     /gene="dgcM"
                     /locus_tag="ACHOQY_000679"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9676829.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            40332..40379
                     /gene="ydaN"
                     /locus_tag="ACHOQY_000680"
     CDS             40332..40379
                     /gene="ydaN"
                     /locus_tag="ACHOQY_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9676830.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            40393..41376
                     /gene="zntB"
                     /locus_tag="ACHOQY_000681"
     CDS             40393..41376
                     /gene="zntB"
                     /locus_tag="ACHOQY_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9676831.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            41651..41824
                     /gene="ynaL"
                     /locus_tag="ACHOQY_000682"
     CDS             41651..41824
                     /gene="ynaL"
                     /locus_tag="ACHOQY_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9676832.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            41854..43227
                     /gene="dbpA"
                     /locus_tag="ACHOQY_000683"
     CDS             41854..43227
                     /gene="dbpA"
                     /locus_tag="ACHOQY_000683"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9676833.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(43356..44291)
                     /gene="ttcA"
                     /locus_tag="ACHOQY_000684"
     CDS             complement(43356..44291)
                     /gene="ttcA"
                     /locus_tag="ACHOQY_000684"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9676834.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(44343..45578)
                     /gene="intR"
                     /locus_tag="ACHOQY_000685"
     CDS             complement(44343..45578)
                     /gene="intR"
                     /locus_tag="ACHOQY_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9676835.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNMWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            complement(45580..45795)
                     /gene="xisR"
                     /locus_tag="ACHOQY_000686"
     CDS             complement(45580..45795)
                     /gene="xisR"
                     /locus_tag="ACHOQY_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFP9676836.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            complement(45895..46083)
                     /locus_tag="ACHOQY_000687"
     CDS             complement(45895..46083)
                     /locus_tag="ACHOQY_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9676837.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(46121..46270)
                     /gene="ralR"
                     /locus_tag="ACHOQY_000688"
     CDS             complement(46121..46270)
                     /gene="ralR"
                     /locus_tag="ACHOQY_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFP9676838.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            complement(46327..47136)
                     /gene="recT"
                     /locus_tag="ACHOQY_000689"
     CDS             complement(46327..47136)
                     /gene="recT"
                     /locus_tag="ACHOQY_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFP9676839.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            complement(47129..49729)
                     /gene="recE"
                     /locus_tag="ACHOQY_000690"
     CDS             complement(47129..49729)
                     /gene="recE"
                     /locus_tag="ACHOQY_000690"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFP9676840.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDYEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            complement(49831..50106)
                     /gene="racC"
                     /locus_tag="ACHOQY_000691"
     CDS             complement(49831..50106)
                     /gene="racC"
                     /locus_tag="ACHOQY_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFP9676841.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            complement(50181..50351)
                     /gene="ydaE"
                     /locus_tag="ACHOQY_000692"
     CDS             complement(50181..50351)
                     /gene="ydaE"
                     /locus_tag="ACHOQY_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFP9676842.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            complement(50351..50572)
                     /gene="kilR"
                     /locus_tag="ACHOQY_000693"
     CDS             complement(50351..50572)
                     /gene="kilR"
                     /locus_tag="ACHOQY_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFP9676843.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            complement(50993..51145)
                     /locus_tag="ACHOQY_000694"
     CDS             complement(50993..51145)
                     /locus_tag="ACHOQY_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9676844.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
     gene            complement(51444..51863)
                     /locus_tag="ACHOQY_000695"
     CDS             complement(51444..51863)
                     /locus_tag="ACHOQY_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9676845.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            51943..52197
                     /locus_tag="ACHOQY_000696"
     CDS             51943..52197
                     /locus_tag="ACHOQY_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9676846.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            52194..52616
                     /gene="ydaT"
                     /locus_tag="ACHOQY_000697"
     CDS             52194..52616
                     /gene="ydaT"
                     /locus_tag="ACHOQY_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9676847.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            52629..53486
                     /gene="ydaU"
                     /locus_tag="ACHOQY_000698"
     CDS             52629..53486
                     /gene="ydaU"
                     /locus_tag="ACHOQY_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP9676848.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKAT
                     NKDTDKDLKTDPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPD
                     SAIPPGFRG"
     gene            53493..54239
                     /gene="ydaV"
                     /locus_tag="ACHOQY_000699"
     CDS             53493..54239
                     /gene="ydaV"
                     /locus_tag="ACHOQY_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9676849.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIEK"
     gene            54262..55023
                     /locus_tag="ACHOQY_000700"
     CDS             54262..55023
                     /locus_tag="ACHOQY_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9676850.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            55039..55461
                     /locus_tag="ACHOQY_000701"
     CDS             55039..55461
                     /locus_tag="ACHOQY_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9676851.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFPSEQARKDWKNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            55458..>55749
                     /locus_tag="ACHOQY_000702"
     CDS             55458..>55749
                     /locus_tag="ACHOQY_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9676852.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT        13895 a        13290 c        14225 g        14339 t
ORIGIN      
        1 caaattgtat taacagttca gcaggacaat cctgaacgca gaaatcaaga ggacaacatt
       61 atgggtattt tttctcgctt tgccgacatc gtgaatgcca acatcaacgc tctgttagag
      121 aaagcggaag atccacagaa actggttcgt ctgatgatcc aggagatgga agatacactg
      181 gttgaagtac gttctacttc ggcgcgcgcg ttggcagaaa agaaacagct gactcgccgt
      241 attgaacaag cgtcggcgcg tgaagttgaa tggcaggaaa aagccgaact ggcgctgctg
      301 aaagagagag aggatctggc acgtgcagcg ttaattgaaa aacagaaact gaccgatctg
      361 attaagagcc tggaacatga agtgacgctg gtggacgaca cgctggcacg catgaagaaa
      421 gagatcggtg agctggaaaa caaattgagc gaaacacgcg ctcgccagca ggcattgatg
      481 ttacgtcatc aggcggcaaa ctcgtcgcgc gatgtgcgtc gtcagctgga cagtggcaaa
      541 ctggatgaag caatggctcg tttcgaatct ttcgaacgtc gtattgacca gatggaagcg
      601 gaagcagaaa gccacagctt cggtaaacaa aaatcgctgg acgatcagtt tgccgaactg
      661 aaagccgatg atgcaatcag cgaacaactg gcacaattaa aagccaaaat gaagcaagac
      721 aatcaataat aacatccagg cggcgtccga acgcgccgcc gctcatcgtc taaggagtac
      781 ttatgagcgc gctatttctg gctattccgt taaccatttt tgtgctgttt gttttaccga
      841 tctggttatg gctgcattac agcaatcgtt ctggtcgcag tgaattgtcg caaagtgagc
      901 agcagcgatt agcgcaactg gctgatgaag caaaacggat gcgcgaacgt attcaggcgc
      961 tggaatctat tcttgatgca gaacatccga actggaggga tcgctaatgg cgggcattaa
     1021 tctcaataaa aaattatggc gtattccaca gcagggcatg gtccgcggcg tctgcgccgg
     1081 gattgccaac tattttgatg taccggcaaa actggtgcgt atcctggtgg tgctgtcgat
     1141 tttcttcggt ctggcgctgt ttaccctggt tgcttacatc attttgtcat ttgcgcttga
     1201 tccaatgccg gacaacatgg cctttggtga gcagctacct tccagcagcg aattgctgga
     1261 tgaagtcgac cgtgaactgg cggcaagtga aacgcgttta cgcgagatgg aacgttatgt
     1321 cacttccgat actttcacgt tacgtagccg tttccgtcaa ctgtgaggaa agttatgaat
     1381 actcgctggc aacaggccgg gcaaaaggta aagcctggtt tcaaattagc aggcaagctg
     1441 gtacttctta ccgcactgcg ctatggcccg gcgggtgtag cgggctgggc gataaaatca
     1501 gttgctcgcc gaccgctgaa aatgttgctg gctgtggcgc tggaaccgct gttaagtcgg
     1561 gctgctaata aactggcaca gcgttataaa aggtgagggg agtttcgcaa aaattgttaa
     1621 atctcaggcg tataatggat ggcaattttc atccatagaa ggacgcttac atgtttaaaa
     1681 aaggcttact tgctctggca ctggtgtttt cactgcccgt tttcgccgct gaacactgga
     1741 tcgatgttcg tgttccagag cagtatcagc aagagcacgt tcagggggcc atcaatattc
     1801 ccctgaagga agtgaaagag cgcattgcca ccgccgttcc ggataaaaac gacaccgtga
     1861 aagtgtattg caatgccgga cgccagtcag ggcaagcaaa agagatcctt agcgagatgg
     1921 gatataccca cgttgagaac gccggtggcc tgaaagacat cgcaatgccg aaggtcaaag
     1981 gttaaaagaa ttcaccatga gcggcgctta tgcgccgttt tttttctgtc acaccttatt
     2041 tacatcccca tagattattt gcgtcagctc acaaatacgc tttttccctg gtaaaaaatg
     2101 atttcctgcg tgactaaaac ccttgtgctc aattgacagt ttattttctg cggagtagtc
     2161 tctcgtttca tgggaccgct accacggaaa ggcaacatga aacagaaaat tacggattac
     2221 ctggacgaaa tctacggtgg aacatttacc gcaactcatt tacagaaact tgtaacgcgt
     2281 cttgagagtg cgaaacgatt aattacacag cgacgtaaaa aacactggga tgaaagtgat
     2341 gtcgtgttaa ttacctatgc cgatcaattt cacagcaatg atttaaaacc attacccaca
     2401 tttaatcagt tttaccatca atggctgcaa agcatttttt cacatgttca tttgttgccg
     2461 ttttatccat ggtcatctga tgatggcttt tcggtaattg attatcatca ggtcgccagt
     2521 gaagcggggg agtggcagga tattcagcaa ctcggtgaat gcagtcattt aatgtttgat
     2581 tttgtctgca accatatgtc ggcaaaaagt gaatgattta aaaactattt acaacagcat
     2641 ccaggttttg aagatttttt tattgccgtt gacccgcaaa ccgatctcag cgccgtcact
     2701 cgcccgcgtg cgttaccgtt attaacgcca ttccagatgc gcgatcattc aacgcgccat
     2761 ttatggacca cctttagtga cgatcaaatt gacctgaatt accgtagccc tgaagtgttg
     2821 ctggcgatgg tggatgtttt actctgttac cttgcgaaag gtgctgagta tgtccgcctg
     2881 gatgccgttg gctttatgtg gaaagagccg ggaacaagct gcatccatct ggaaaaaaca
     2941 catctgatta tcaaactgtt acggtcgatt attgataacg ttgcgccagg tacagtgatc
     3001 attaccgaga ccaatgttcc gcataaagac aacattgctt actttggcgc aggcgatgac
     3061 gaagcacata tggtgtacca gttctcgctg ccgccactgg tgctgcatgc ggtgcaaaaa
     3121 cagaacgttg aggcgctttg tgcgtgggcg caaaacctga cactaccttc cagcaacacc
     3181 acctggttta acttcctcgc ctctcacgat ggcatcgggc taaacccgct acggggcttg
     3241 ttgcctgaaa gcgaaatatt agagctggtc gaggcgttac agcaggaagg tgcattagta
     3301 aactggaaaa ataatcccga cggtacacgc agtccgtatg aaataaatgt gacctatatg
     3361 gatgcgttaa gccgccgtga gagtagcgat gaagaacgtt gcgccaggtt tattctcgcc
     3421 catgcgattt tgttaagttt ccccggtgtg ccagcgatat atattcaaag tattcttggc
     3481 tcgcgtaatg attacgcagg tgtcgaaaaa ctcggatata accgtgcgat taaccgtaaa
     3541 aaatatcaca gtaaagagat aacccgagaa ctgaacgatg aagctacatt aaggcatgcg
     3601 gtatatcatg agttgtcgcg tttaattaca cttcgtcgca gccataacga gtttcatccg
     3661 gataataatt ttaccatcga tacgattaat tcatccgtaa tgcgtattca aagaagtaac
     3721 gctgatggta attgtctgac tggattgttt aatgtcagta aaaatattca gcatgtaaat
     3781 attactaatc tgcatggtcg ggatctgatt agtgaagttg atatattggg taatgaaata
     3841 acgctgcgcc cctggcaggt tatgtggatt aaataaaaag gaacatctca tgattaaatc
     3901 aaaaatcgtg ctgttatcag cactggtttc ctgcgccctg atttcagggt gtaaagaaga
     3961 aaataaaacg aatgtatcca tcgaatttat gcattcttcg gtggagcagg agcgccaggc
     4021 cgttatcagt aaattgattg cccgttttga aaaagaaaac cctggcatca ccgttaagca
     4081 agtgcccgtg gaagaagatg cctataacac taaagtcatt actctttcac gtagcggttc
     4141 gctgccggaa gtgatagaaa ccagccatga ctacgccaaa gtgatggaca aagagcagct
     4201 tatcgatcgc aaagcggttg ccacagtcat cagcaacgtt ggtgaaggcg cgttttacga
     4261 tggcgtactg cgtattgtgc gtaccgaaga tggtagcgca tggaccggtg ttcctgtcag
     4321 cgcctggatt ggcggtatct ggtatcgcaa agatgtgctg gcaaaagcgg ggcttgagga
     4381 gccgaaaaac tggcaacagc tgctggacgt tgcacagaaa ctgaatgacc cggcgaataa
     4441 aaaatacggc attgcgctgc ctacagcaga aagcgtgttg acggaacaat ccttctccca
     4501 gtttgcgtta tccaaccagg ctaacgtctt taacgccgaa ggcaaaatca cccttgatac
     4561 accagagatg atgcaggcac tgacctatta ccgcgacctt gctgccaaca ctatgccggg
     4621 ttctaacgac atcatggaag tgaaagacgc ctttatgaac ggcaccgcgc cgatggcgat
     4681 ttactccacc tatatccttc cggctgtgat taaagaaggc gacccgaaaa acgtcggttt
     4741 cgtggtgcca accgagaaaa actctgcggt ctacggcatg ttgacctcgc tgaccattac
     4801 cgccgggcaa aagaccgaag agacggaagc agcagaaaaa tttgtcacct ttatggagca
     4861 ggcagacaac attgccgact gggtgatgat gtcgccaggt gctgcgctgc cggtgaataa
     4921 agcggttgtc actaccgcca cctggaaaga caacgacgtt attaaggcgc tgggtgaact
     4981 accgaatcag ctaatcggtg aactgccaaa tattcaggtc tttggtgcag taggggataa
     5041 aaactttacc cgcatgggtg atgtgacggg gtctggcgtg gtgagctcca tggtgcataa
     5101 cgtcaccgtg ggtaaagccg atctccctgg cacgctgcaa gcgagccaga aaaagctgga
     5161 tgaactgatc gaacagcact aaacccagga caggaatccg caatgaacag gcttttttca
     5221 ggtcgttccg atatgccctt tgcgctgctg cttctcgcgc ccagcttatt actgctgggt
     5281 ggtctggtgg cctggccaat ggtgtcgaat atcgaaatca gttttttacg tctgccgctc
     5341 aatcccaaca tcgagtcaac ctttgttggg gtgagtaact atgtgcgtat cctctccgat
     5401 cccggcttct ggcactcgct gtggatgacg gtctggtata ccgcgctggt ggtggcgggc
     5461 agcaccgtgc tcgggctggc agtggcgatg tttttcaacc gcgagttccg tctgcgcaaa
     5521 accgcgcgtt cgctggtgat cctctcctac gtaacgccgt ccatttcgct ggtgtttgcc
     5581 tggaaataca tgttcaacaa cggctacggc attgtgaact acctcggcgt cgatcttctg
     5641 catctctatg agcaggcacc gctgtggttc gataatccgg gcagtagctt tgtgctggtg
     5701 gtgctgttcg ccatctggcg ctacttcccg tatgccttta tctcgtttct ggcgattttg
     5761 cagaccatcg acaaatcgct gtacgaagcg gcagagatgg atggcgctaa tgcctggcaa
     5821 cggtttcgta tcgtcacgct gcccgcgatt atgccggtcc tggcgacggt ggtgaccctg
     5881 cgcaccatct ggatgttcta catgttcgcg gatgtttatt tgctgacgac caaggtcgat
     5941 attctcggtg tatatctcta caaaaccgcc tttgccttta atgatttagg aaaagcggcg
     6001 gcgatctcgg tggtgctctt catcattatt ttcgctgtca ttctgctgac caggaaacgg
     6061 gtgaacctca atggcaacaa ataaacgcac actcagtcgc atcggttttt actgcgggct
     6121 ggcgctgttt ctcatcatca cgctgtttcc attttttgtg atgctgatga cctcgttcaa
     6181 gggcgcgaaa gaggcgatct cactgcatcc tacgctgctg ccgcagcagt ggacgctgga
     6241 gcattacgtc gacattttta acccgatgat tttcccgttt gtcgactact tccgtaacag
     6301 tctggtggtg tcggtggttt catccgtggt ggcggtattc ctcggcattc ttggggctta
     6361 cgcgctttcc cgcctgcgct ttaaaggtcg gatgaccatc aatgccagct tttacacggt
     6421 gtatatgttc tctggcattt tgctggtggt gccgctgttc aaaatcatca ccgcgctggg
     6481 catttacgac accgagatgg cgctgatcat caccatggtg acgcaaactc tgcccaccgc
     6541 cgtgtttatg ctgaaaagct acttcgacac catccccgat gagatcgaag aagcggcgat
     6601 gatggatggc ctcaaccgtc tgcaaattat cttccgcatc accgtgccgt tggcgatgtc
     6661 cgggctgata tccgtgttcg tctactgctt tatggtggcg tggaacgact atctgtttgc
     6721 gtcgattttc ctctccagcg ccagcaattt caccttaccg gtgggcctga acgcgctgtt
     6781 cagtacgcca gattacatct ggggacggat gatggcggcc tcactggtga ccgcattgcc
     6841 ggtggtgatt atgtatgcgc tttcggaacg ttttattaag agtggtttga ccgccggtgg
     6901 cgtgaagggc taaagcggcc agtttcttta caaggagttt taaatgaaaa agttagtagc
     6961 cacagcaccg cgtgttgctg cgctggttga gtatgaagat cgggcgattt tagccaatga
     7021 agtgaaaatc cgcgtgcgtt tcggcgcacc gaaacacgga acggaagtgg tcgacttccg
     7081 cgccgccagc ccgtttatcg atgaagactt taacggcgaa tggcagatgt tcactccgcg
     7141 tccggcagat gcgccgcgcg gcattgagtt tggcaaattc cagcttggca acatggtggt
     7201 tggcgacatt atcgagtgcg gcagcgacgt taccgactac gcggtgggcg acagcgtatg
     7261 cggctacggc ccgctctccg agacggtcat cattaacgcg gtgaataact acaagctgcg
     7321 caaaatgccg gaaggcagct cgtggaaaaa cgccgtttgt tacgacccgg cgcagtttgc
     7381 catgagcggc gtgcgcgatg ccaacgtgcg cgtgggtgat tttgtggtgg tggtagggct
     7441 tggcgcgatt ggtcaaatag ccatccaact ggcgaaacgc gctggcgcgt cggtggtgat
     7501 tggcgtcgat cctatcgcct atcgctgtga tattgcccgt cgccacggtg ctgatttctg
     7561 ccttaacccc attggcactg acgtaggaaa agagatcaaa acgctgaccg gcaagcaggg
     7621 tgccgatgtg attatcgaaa ccagcggtta cgccgacgcg ctgcaatcgg cgctgcgtgg
     7681 ccttgcttat ggcggcacca tctcttatgt cgcgtttgcc aaaccatttg ctgaaggttt
     7741 taacctcgga cgcgaagcgc atttcaataa cgccaaaatt gttttctccc gtgcgtgcag
     7801 cgaaccgaac ccggattatc cgcgctggag ccgtaagcgt attgaagaaa cctgctggga
     7861 actgctgatg aacggttatc tcaattgcga agatttaatc gacccggtag tgacctttgc
     7921 caagagcccg gaaagctaca tgcagtatgt cgatcagcat ccggaacaga gcatcaaaat
     7981 gggcgttacg ttttaattca aaggaatgaa aaaatgaaaa taggcacaca gaatcaggca
     8041 ttctttccgg aaaacattct ggagaaattt cgttatatca aagagatggg cttcgatggt
     8101 tttgagattg acggcaaact gctggtcaac aatctcgaag aagtcaaagc agcaatcaaa
     8161 gaaaccggtt taccggtgac caccgcctgc ggtggctatg acggttggat tggcgacttt
     8221 atcgaagagc gtcgtcttaa tggcttaaag cagatcgaac gcattctcga agcgctggca
     8281 gaagtggggg gtaaaggtat cgtcgttccg gcagcgtggg gcatgtttac cttccgctta
     8341 ccgccgatga cctcgccgcg tagcctggac ggcgaccgca aaatggtgag tgattccctg
     8401 cacgtactgg aacaggtcgc cgcgcgtacc ggaaccgtgg tgtatctcga accgttaaac
     8461 cgctatcagg atcatatgat caacaccctc gccgatgccc gccgttacat cgtcgaaaac
     8521 gatcttaaac atgtacagat tatcggcgat ttctatcaca tgaatatcga agaagataac
     8581 ctggcgcagg cgctgcatga caaccgcgac ctgctcggtc atgtgcatat tgcggataac
     8641 catcgctacc agccgggcag cggcaccctg gatttccacg cgctgtttga acagctgcgc
     8701 gcggataact atcagggcta tgtagtgtac gaagggcgta tccgggcgga agagcctgcc
     8761 caggcgtacc gtgattcgtt ggcctggttg cgtacctgct aagaggtctt tgtgaaaagt
     8821 gcaatgacaa gctctccgct gcgggtcgcg ataataggcg caggccaggt ggcggataag
     8881 gttcatgctt cgtactactg cacccgcaac gatctggaac tggtggctgt ctgtgacagc
     8941 cgcctttccc aggcgcaggc gctggcagat aaatacggta atgcatccgt gtgggacgat
     9001 ccgcaggcca tgctgctggc ggtgaaacct gatgtggtta gcgtctgctc acctaaccgt
     9061 tttcattacg aacataccct gatggcactg gaagcgggct gccatgtgat gtgcgaaaaa
     9121 ccgcccgcca tgacgccaga acaggcgcgg gaaatgtgcg ataccgcgcg caaactgggc
     9181 aaggtgctgg cctacgactt tcaccaccgt tttgcgctcg atacgcaaca gctgcgtgaa
     9241 caggtgacca acggcgtttt gggagagatt tacgttacca ccgcccgcgc cctgcgtcgc
     9301 tgcggcgttc ccggctgggg tgtctttacc aataaagaac tgcagggtgg tggcccgctt
     9361 atcgacatcg gcattcatat gctggatgcg gcgatgtatg tgctgggctt tccggcggtg
     9421 aaaagcgtga atgcgcatag ctttcaaaag atcggcacgc aaaagagctg cggtcaattt
     9481 ggcgagtggg atccggcaac ttacagcgtc gaagattcgt tatttggcac cattgaattt
     9541 cataacggcg gcattctgtg gctggaaacg tcatttgcac tcaacatccg cgaacagtcg
     9601 attatgaacg tcagcttttg tggtgataaa gccggtgcga cgctgtttcc agcacatatc
     9661 tacaccgata acaacggtga attaatgacg ctgatgcaac gggaactggc agacgacaac
     9721 cgccatttgc gcagcatgga agcctttatc aatcacgtac agggcaagcc cgtgatgata
     9781 gccgacgccg agcaggggta catcatccag caactggtgg cggcgttgta tcaatccgca
     9841 gaaacaggga cgcgtgtgga attatgacca ggccagtaac gttatcagaa ccccatttca
     9901 gccagcatac cctgaacaag tatgcatcgc tgatggcgca ggggaacggc tatcttgggc
     9961 ttcgcgccag ccatgaagaa gattacacgc gccagacgcg agggatgtat ctggcggggc
    10021 tgtatcatcg ggcgggaaaa ggtgaaatca acgaactggt gaacctgcct gatatcttgg
    10081 ggatggagat tgccataaat ggtgaggttt tctcgttatc ccgcgaagcc tggcagcgtg
    10141 aacttgactt tgccagtgga gaattacgcc gtagcgttgt ctggcgtacc agcaacggca
    10201 caggttacac catcgccagc cgtcgctttg tttcggcaga ccaactgccg ctcattgtgc
    10261 tggaaatcac tattacgcca ctggacgccg acgcgtcagt gctgatttca acaggtatcg
    10321 acgccacgca aaccaaccac ggtcgccaac atctcgacga aacccaggtg cgggtgtttg
    10381 gtcagcatct gatgcagggg atctacacca cccaggatgg acgcagtgat gtggccatca
    10441 gctgttgctg tatggtgagc ggtgatgtgc agcaatgcta taccgccaaa gagcgccgtt
    10501 tgcagcaaca taccagtgcg cagcttcatg caggcgagac agtgacgttg caaaaactgg
    10561 tgtggatcga ctggcgggat gacaggcaag ccgttttaga cgagtggggc agcgcgtcgc
    10621 ttcgccagct tgaaatgtgc gcgcagcaga gttacgacca acttcttgca gtatcaacag
    10681 aaaactggcg tcaatggtgg cagaaacgtc gtatcacggt aaatggcggc gaagcgcacg
    10741 atcagcaagc gttagattat gcgctttatc atctgcgcat catgacgcct gcccacgacg
    10801 agcgcagcag cattgcggca aaaggcttaa ccggcgaagg ctacaaaggc cacgttttct
    10861 gggatacaga agtatttttg ttaccgtttc atctgtttag cgatccgacg gttgcccgaa
    10921 gtttactgcg ttatcgctgg cacaacttgc caggcgcgca ggagaaagcg cgacgcaacg
    10981 gctggcaggg cgcgctattt ccgtgggaaa gcgcgcgcag cggcgaagaa gagacgccgg
    11041 aatttgccgc cattaacatt cgcaccgggc tgcggcaaaa agtggcctcg gcgcaggcgg
    11101 aacatcatct ggtggccgat atcgcctggg cggttattca atactggcag accacggggg
    11161 atgaaagttt cattgcgcat gaaggcatgg cgctacttct ggaaactgca aagttctgga
    11221 ttagccgcgc ggtgagggtt aacgaccgtc tggaaattca tgatgttatt gggcctgacg
    11281 aatataccga acatgtcaat aataacgcct tcaccagcta tatggcgtat tacaacgtcc
    11341 agcaggcgct gagtattgcc cgccagtttg gctgtagcga cgatgcgttt atccatcgcg
    11401 ccgaaatgtt ccttaaagaa ctgcggctgc cagaaattca gcccgacggc gttttgccgc
    11461 aggatgattc gtttatggct aagccggcga ttaatctggc gaaatacaaa gcggcggcgg
    11521 ggaagcaaac cattctgctg gattattcac gcgcagaagt gaacgagatg caaatcctca
    11581 aacaagctga tgtggtgatg ctcaattaca tgctgccgga gcagttctca gcggcatcgt
    11641 gtcttgccaa tctgcaattt tatgaaccgc gcactattca cgactcgtca ttaagtaaag
    11701 caatccacgg cattgttgcc gcacgctgtg gcctgctgac ccaaagttat cagttctggc
    11761 gcgaggggac tgaaatcgat cttggtgctg atccgcatag ttgtgatgat ggtatccatg
    11821 ctgccgcaac tggcgctatc tggctggggg cgattcaggg ttttgccggg gtgagcgtgc
    11881 gtgacggtga attgcatctc aatccggcgt tacctgagca gtggcaacag ttgtctttcc
    11941 ctctgttctg gcagggctgc gaattacagg tcactcttga cgcgcagcgt attgcgattc
    12001 gaacttctgc gcccgtttca ctgcgtttga acggtcagct tatatccgtg gctgaagaat
    12061 ctgttttctg tttgggtgat tttattttgc ccttcaatgg gaccgctacc acacatcagg
    12121 aggatgaatg aaactgcaag gggtaatttt cgatctggat ggtgtaatca ccgataccgc
    12181 gcatctgcat ttccaggcgt ggcagcagat tgccgctgaa attggcatca gcattgatgc
    12241 gcagtttaac gaatccctaa aagggatcag ccgcgatgag tctctgcggc gcattctgca
    12301 acacgggggc aaagagggcg actttaactc gcaggagagg gcgcaactgg cgtatcgcaa
    12361 aaatctgctc tatgtccact cactgcgcga gttgacggtc agcgctgttc tacccggcat
    12421 tcgctctttg ctggcagatc tccgtgcaca gcagatcccg gttgggctgg cttctgtctc
    12481 cctgaatgcg ccgacgattt tagcggcgct ggagctgcgc gagtttttca ccttctgcgc
    12541 ggatgcttcc caacttaaaa actcgaaacc ggacccggaa atctttctcg ccgcctgtgc
    12601 cgggctgggc gtgccgccgc aggcatgtat cggcattgaa gatgcgcagg cgggcattga
    12661 cgccattaac gccagcggta tgcgctcggt ggggatcggc gcgggcttaa ccggggcgca
    12721 attactgttg ccttcaacgg actctctgac ctggtcgcgg ttatcggcct tctggcaaaa
    12781 cgtatagcaa aggaatcaac atggctcagc tttcgttaca acatattcaa aaaatctacg
    12841 ataaccaggt gcatgtggtg aaggacttca acctggaaat tgccgataaa gagttcatcg
    12901 tgtttgtcgg cccgtcgggc tgcggtaagt cgaccaccct gcgcatgatt gccgggcttg
    12961 aggagatcag cggcggcgat ctgttgatcg acggcaaacg aatgaatgac gttccagcca
    13021 aagcacgcaa tatagcgatg gtgttccaga actacgcgtt gtatccgcat atgacggttt
    13081 acgacaacat ggcgtttggc ctgaagatgc aaaaaatcgc caaagaggtg attgatgagc
    13141 gggtgaactg ggcggcgcaa attctcggcc tgcgtgagta cctgaaacgc aagccgggcg
    13201 cgctttccgg cgggcaacgc cagcgtgtgg cgctcgggcg ggcgattgtg cgcgaagcgg
    13261 gcgtgttttt aatggatgaa ccgctctcta accttgatgc caagctgcgc gtgcaaatgc
    13321 gcgcagagat cagcaagcta caccagaagc tgaataccac catgatctac gtgacccacg
    13381 atcagaccga agcgatgacc atggcgacgc ggattgtgat tatgaaggat gggattgttc
    13441 agcaagttgg tgcgccgaaa acggtttata accaaccagc gaatatgttt gttgccggat
    13501 ttattggatc accggcgatg aattttattc gtggcacgat cgatggcgat aaattcgtta
    13561 cggaaacgct taaattaacc attcccgaag agaaattagc ggttctgaaa acacagggaa
    13621 gtttgcataa gcccatcgtg atgggaatac gtccggaaga tattcatccg gacgcgcaag
    13681 aggaaaataa catttccgcc aaaattagcg tggcagaatt aaccggtgcg gaatttatgc
    13741 tctacaccac ggttgggggg cacgagttag tggtccgtgc tggtgcgtta aatgattatc
    13801 atgcaggaga aaatatcact attcattttg atatgacgaa atgtcatttc tttgatgcag
    13861 aaacggaaat agcaattcgc taaatacagg gggaaggcat tcccccagaa taatacaagg
    13921 aacaataatg aaaaagttat taccctgtac cgcactggtg atgtgtgcgg gaatggcctg
    13981 cgcacaggcc gaggaaagga acgactggca ctttaatatc ggcgcgatgt acgaaataga
    14041 aaacgtcgag ggttatggcg aagatatgga tgggctggcg gagccttcag tctattttaa
    14101 tgccgccaac gggccgtgga gaattgctct ggcctattat caggaagggc cggtagatta
    14161 tagcgcgggt aaacgtggaa cgtggtttga tcgcccggag ctggaggtgc attatcagtt
    14221 cctcgaaaac gatgatttca gtttcggcct gaccggcggt ttccgtaatt atggttatca
    14281 ctacgttgat gaaccgggta aagacacggc gaatatgcag cgctggaaaa tcgcgccaga
    14341 ctgggatgtg aaactgactg acgatttacg tttcaacggt tggttgtcga tgtataaatt
    14401 tgccaacgat ctgaacacga ccggttacgc tgatacccga gtcgaaacgg aaacaggcct
    14461 gcaatatacc ttcaacgaaa cggttgcctt gcgagtgaac tattatctcg agcgcggctt
    14521 taatatggac gacagccgca ataacggtga gttttctacg caagaaattc gcgcctattt
    14581 gccgctgacg ctcggcaacc actcggtgac gccgtatacg cgcattgggc tggatcgctg
    14641 gagtaactgg gactggcagg atgatattga acgtgaaggc catgatttta accgtgtagg
    14701 tttattttac ggttatgatt tccagaacgg gctttccgtt tcgctggaat acgcgtttga
    14761 gtggcaggat cacgacgaag gcgacagcga taaattccat tatgcaggtg tcggcgtaaa
    14821 ttactcgttc tgataatggg ctaaattgcc ggatgcggca cgagtacttt atccgaacta
    14881 taaatgtagg ccggataaga tgcgctagca tcgcatccgg cattcaggca aggtagctgg
    14941 tatttatttc agcgtcatat gcgtggcaac ggtaatattc tgtggtgacg gttttccaga
    15001 aattaagcgg aataataact cacagctttg ttgacctaac tcctgtgttg ggatatcaat
    15061 accgcctggc gcaggcgtta aaataaacga cagcgtttca ttactataac ccaccaccgc
    15121 taactgctgc ggaatagcaa tatttttctc tgctgccgca cgataaatac tcattaattt
    15181 caggctgtca gtagcaaaca ctgcctcagg caacggtgac tggcttaata attcccgcgc
    15241 ggcttgcagt gctgtttcat gggtataacc gccgtcaaca atccattcat cacgcactgc
    15301 aatattatgc gcagccaggc tctgcttata accatttacc cgatccactg aaacatggac
    15361 atcaagcgga gcatgcaggc aggcaatatt ttgatgccca ctttcaatta atgcatcggt
    15421 caacgcaatg ctgtcgccaa aattatcggt atcgacagaa taaacatggg catattgacc
    15481 ttcaactttg ccaatcacca cgacaggaat atcgtattta tcaagttggg caaaaaatga
    15541 ctcatccgct ggcgaactta gcataataat gcctttaatc attttctgct taattttgct
    15601 ttcacatttt tgtaagtctt ctgccggatt gtgcgacgtc tgcaatatca cgtcgaaacc
    15661 ttcctcttca gctttggcgg tgatggcatg caaaacttca gagaaaaacg gattacccgc
    15721 cgtagttttg gtcgaacggg tagaaatcac cataatggca tcaaaacccg aagaggtcag
    15781 cgcgcgggcc agcttgtttg gctgatactg taattcttca atggcccgta acactttttc
    15841 gcgcgcttcc ggggagatat tagtttgctt attcagcacg cgtgatacgg tggattttga
    15901 tacgcctgca accctggcaa tatcataaat agtaggggac ataggtcagg aactccgtcc
    15961 gaacgtgaat gctgcacatc ttatggagtt cgcccgtcga tgacaacacg ggcagttgat
    16021 aatcaatggc ctggccccca cattcatatc cttacgaatg atttttttct ggtcttcaga
    16081 gcagcacagg acagcaatga agcgacttaa aaatgaactt aatgcgctgg tgaatcgggg
    16141 tgtcgacaga catctgcgcc tcgccgtaac cggactaagc cgcagcggca aaacagcgtt
    16201 tatcactgcg atggtcaatc agttgctcaa tattcacgcc ggagcacgct tgccgctgtt
    16261 aagcgcagtg cgtgaagagc gcctgctggg cgtaaaacgc attcctcagc gtgactttgg
    16321 cattccgcgc ttcacatatg atgaaggact ggcgcagtta tatggcgatc ctcccgcctg
    16381 gccgacgcca acgcgcggcg tcagtgaaat ccgcctggcg ctacgtttta aatcgaacga
    16441 ttcgctgcta cgccacttca aggacacctc cacgctgtat ctggagattg tggattatcc
    16501 cggcgaatgg ttgctcgacc tgccgatgct ggcgcaggac tatttaagct ggtcacgcca
    16561 gatgacgggc ttactcaatg gtcagcgcgg cgaatggtcg gtcaaatggc gaatgatgtg
    16621 cgaagggctg gacccgctag cgcctgccga cgaaaaccgg ctggcagaca ttgccgccgc
    16681 gtggaccgat tatctccacc actgtaaaca gcaggggctg cactttattc agcctgggcg
    16741 ctttgtcttg ccgggagata tggcaggtgc gcccgcgctg caattcttcc cgtggccgga
    16801 tgtcgatgcc tggggcgagt ccaaactggc gcaggccgat aagcacacca atgccggaat
    16861 gctgcgcgag cggtttaatt attactgcga gaaggtggtg aaggggttct ataagaatca
    16921 ttttctgcgc tttgaccgcc agattgtgct ggttgattgc ctgcaacctc tcaacagtgg
    16981 gccacaggca tttaatgata tgcgtctggc actgacgcag ctgatgcaaa gtttccacta
    17041 cgggcagcgt accttgttcc ggcgtttgtt ttcgccggtt atcgataagc tattgtttgc
    17101 tgccactaaa gcggaccatg tgaccatcga tcagcacgcc aatatggttt cattactgca
    17161 acaactgatt caggatgcct ggcaaaatgc ggcgtttgaa gggattagta tggattgcct
    17221 ggggctggcc tcggtgcagg cgaccaccag cggcattatt gacgtcaacg gcgagaaaat
    17281 tccggcattg cgcggtaacc ggctcagcga tggtgcgcca ctcaccgttt atcccggtga
    17341 agttcctgcg cgtttgcccg gtcaggcgtt ctgggacaag caagggttcc agtttgaagc
    17401 gtttcgcccg caggtgatgg atgtcgacaa accgctgccg catattcgtc ttgatgccgc
    17461 gcttgaattt ttaataggag ataaattgcg atgaccgaac cgttaaaacc acgtattgat
    17521 ttcgacggtc cgctggaggt cgatcagaat ccaaaattca gggcgcagca gacctttgac
    17581 gaaaatcagg cgcaaaattt tgccccggcc acgctcgatg aagcgcctga agaagagggg
    17641 caagttgaag cggtaatgga tgcagcgtta cgtccgaaac gcagcctgtg gcgcaaaatg
    17701 gtgatgggcg ggctggctct gtttggcgca agcgttgtcg ggcagggtgt acagtggaca
    17761 atgaatgcct ggcaaactca ggactgggtg gcgctgggtg gatgtgccgc tggggcattg
    17821 attatcggcg ctggcgtagg ttctgtggta acagagtggc ggcgcttatg gcgcttgcga
    17881 cagcgcgccc atgaacgcga cgaagcgcgc gatttgttgc acagccacgg cacgggcaaa
    17941 ggccgcgcat tttgcgaaaa actggcgcag caggcgggta ttgatcagtc tcatccagcg
    18001 ctgcaacgct ggtatgcctc aatccatgaa acgcaaaacg accgtgaagt ggtcagtttg
    18061 tatgcgcatt tggtccagcc agttttagat gcccaggcgc ggcgcgaaat cagccgttcg
    18121 gcggcggaat caacgttgat gattgcggtc agcccgctgg cgttggtcga tatggcgttt
    18181 atcgcctggc gcaatctgcg tttaattaat cgcatcgcca cgctgtatgg cattgaactg
    18241 gggtattaca gccgtttgcg tctgtttaag ctggtattgc tgaatatcgc ttttgccgga
    18301 gccagcgaac tggtgcgcga agtggggatg gactggatgt cgcaagatct cgctgctcgt
    18361 ttgtctaccc gcgcagctca gggaattggt gctggacttc tgacggcacg actcgggatt
    18421 aaagctatgg agctttgccg cccgctgccg tggattgacg atgacaaacc tcgcctcggg
    18481 gatttccgtc gtcagcttat cggtcaggtg aaagaaacgc tgcaaaaagg caaaacgccc
    18541 agcgaaaaat aatgcaatat cgggtgctga ccggatatct ttacgccgaa gtgcccgttt
    18601 ttccgtcttt gtgtcaatga ttgttgacag aaaccttcct gctatccaaa tagtgtcata
    18661 tcatcatatt aattgttctt ttttcaggtg aaggttccca tgcgtctgga agtcttttgt
    18721 gaagaccgac tcggtctgac ccgcgaatta ctcgatctac tcgtgctaag aggcattgat
    18781 ttacgcggta ttgagattga tcccattggg cgaatctacc tcaattttgc tgaactggag
    18841 tttgagagtt tcagcagtct gatggccgaa atacgccgta ttgcaggtgt taccgatgtg
    18901 cgtactgtcc cgtggatgcc ttccgaacgt gagcatctgg cgttgagcgc gttactggag
    18961 gcgttgcccg aacctgtgct ctctgtcgat atgaaaagca aagtggatat ggcgaacccg
    19021 gcgagctgtc agctttttgc gcaaaaattg gatcgcctgc gcaaccatac cgccgcacaa
    19081 ttgattaacg gctttaattt tttacgttgg ctggaaagcg aaccgcaaga ttcccataac
    19141 gagcatgtcg ttattaatgg gcagaatttc ctgatggaga ttacgcctgt ttatcttcag
    19201 gatgaaaatg atcaacacgt cctgaccggt gcggtggtga tgttgcgatc aacgattcgt
    19261 atgggccgcc agttgcaaaa tgtcgccgcc caggacgtca gcgccttcag tcaaattgtc
    19321 gccgtcagcc cgaaaatgaa gcatgttgtc gaacaggcgc agaaactggc gatgctaagc
    19381 gcgccgctgc tgattacggg tgacacaggt acaggtaaag atctctttgc ctacgcctgc
    19441 catcaggcaa gccccagagc gggcaaacct tacctggcgc tgaactgtgc gtctataccg
    19501 gaagatgcgg tcgagagtga actgtttggt catgctccgg aagggaagaa aggattcttt
    19561 gagcaggcga acggtggttc ggtgctgttg gatgaaatag gggaaatgtc accacggatg
    19621 caggcgaaat tactgcgttt ccttaatgat ggcactttcc gtcgggttgg cgaagaccat
    19681 gaggtgcatg tcgatgtgcg ggtgatttgc gctacgcaga agaatctggt cgaactggtg
    19741 caaaaaggca tgttccgtga agatctctat tatcgtctga acgtgttgac gctcaatctg
    19801 ccgccgctac gtgactgtcc gcaggacatc atgccgttaa ctgagctgtt cgtcgcccgc
    19861 tttgccgacg agcagggcgt gccgcgtccg aaactggccg ctgacctgaa tactgtactt
    19921 acgcgttatg cgtggccggg aaatgtgcgg cagttaaaga acgctatcta tcgcgcactg
    19981 acacaactgg acggttatga gctgcgtcca caggatattt tgttgccgga ttatgacgcc
    20041 gcaacggtag ccgtgggcga agatgcgatg gaaggttcgc tggacgaaat caccagccgt
    20101 tttgaacgct cggtattaac ccagctttat cgcaattatc ccagcacgcg caaactggca
    20161 aaacgtctcg gcgtttcaca taccgcgatt gccaataagt tgcgggaata tggtctgagt
    20221 cagaagaaga acgaagagta agcgcgaata tgcctgatgg tgcaacacca tcaggcatat
    20281 taacttatgc tttcagtaca gccagagctg cttcgtaatc cggctcggtg gtgatttcat
    20341 ccaccagctg gctgaaaatc acattgtcat tttcgtcaat aaccacaacg gcacgcgctg
    20401 ccagaccttt cagtgggcca tcagcaattg ccacaccgaa ggcttgcaga aattcagcgt
    20461 tacggaaagt ggagagggtg ataacgttgt tcagaccttc tgcgccgcag aaacgagact
    20521 gggcgaacgg cagatcggca gagatacaca gcacaacggt gttgtcgatc tcagttgcca
    20581 gttggttaaa cttacgtact gatgcggcgc aaacaccggt atcaatactc gggaaaatgt
    20641 tcagcacttt gcgtttaccc gcaaactgac cgagggtgac gtcagacaga tcttttgcca
    20701 cgagagtaaa agtctgcgct ttgctacccg cctgcgggat ggaattggcg actgtaaccg
    20761 ggttgccctg gaaatgaacg gtttgtgaca tgattatctt tcctgtttac atatagttaa
    20821 cgtcacacct agtttatgct aactgtcaat aacacagcaa acgctatttg cgcttaatcc
    20881 gcagaccacc gcgacaacaa ggagtaaaga tgagaaccgt taaggtattt gaggaagcct
    20941 ggcccttaca taccccgttt gtgattgccc ggggaagtcg cagtgaagcg cgcgtggtgg
    21001 tggttgaact ggaagaagag ggtattaaag gcaccggcga atgcacgccg tatccgcgtt
    21061 atggggaaag tgatgcctcg gtaatggcgc aaattatgag cgtcgtgccg caattagaga
    21121 aagggctgac acgggaggag ttgcaaaaaa ttctccctgc cggcgcagca cgtaatgcgc
    21181 tggattgtgc attgtgggat ctggccgcgc gaaaacagca gcaatcgctg gctgatttga
    21241 tcggcataac gcttcccggg acagtcacca ctgcacagac ggtagtcatc ggtacgcctg
    21301 atcagatggc caatagtgca tcaacactct ggcaggcagg cgcgaaatta ctgaaagtga
    21361 agctggataa ccatcttatc agtgagcgga tggtggcaat tcgcacagct gtgcccgatg
    21421 cgacgctgat cgttgacgca aatgaatcct ggcgtgcaga agggttggcg gcgcgttgcc
    21481 agctattggc ggatttaggc gttgcgatgc ttgaacaacc gcttcctgcg caggacgatg
    21541 cggcgctgga gaattttatt catccgttac cgatttgtgc agatgaaagt tgtcatacac
    21601 gtagcaatct gaaggcgctg aaagggcgct atgagatggt taacattaag cttgataaaa
    21661 ccggtggtct gacggaagcg ctggcgctgg cgactgaagc gcgtgcacaa ggttttcgtc
    21721 tgatgctggg ctgcatgttg tgtacctctc gggccattag cgccgcttta ccgctggtgc
    21781 cgcaggtcag tttcgtcgat cttgacggac cgacctggct ggcggtagat gtggaaccgg
    21841 cgcttcagtt cacgacgggc gaattgcatc tttaggatgc cagcgcagca agtttgccat
    21901 cgcaaagaga tatttttcgc tggcttcgtc ggaggagatg ggcggaaact ctgcggtgat
    21961 gcaatgcagg ttgagatccg cacaccaact accgaaagag ccaggtgttt cataaccgac
    22021 gctggttacc agaggcaatt caaacgcctg ggccagccat tcgcctaatt cgctgtgtct
    22081 gggatcttca atacaggcca gcggatcgtg gaatgagacc acccaggcgg gttgaatgcg
    22141 gtgtataagc tggcacagcg cctgggtttc aggttcagag ccaggtttgt cacctgtcag
    22201 caatacgaca tcacgttctt cagcggcgct gttccagcga taaactgttt caccttcctt
    22261 ccagttcgcc gccggaaagt ttcggtttaa atccacacca ttagcattgg cccgtaaccc
    22321 cagctggcag ccgtcaggat tcacacacag cactacatga tggcggcgca aagaaggtgt
    22381 caaagtccgc agagcacagg agagcgtgac gaccgaagag ttttcatcac cgtgagtgcc
    22441 agccagaatc aaaccacttt cgcgactggc tgcagtggcc ggaaaccaga ttaacggcgc
    22501 gcccaataat gaacgtccgt aatgttctgt tccgggcgga aatgcgccgc gttcggcgcg
    22561 tgggcggttt acggtcatga tcgtctctgg ttcaggaagt attacaggca gtgttgtgca
    22621 aattttgcgg attatcaaat ccttttcttc aacgctatgt catcgatctt gtatcacttt
    22681 tttgccggaa gttgtttgca tttttattga tgtgcagata ttagaattat gctgacttag
    22741 atgtaagtcg taatgttata tacatggcgt attttattct tactggttct acacatgtta
    22801 atcgcatcgg attgatcagt tcaccttaat aagtttaata aatcgtactg cacatagtac
    22861 attacgatgg taactatgta attgatttac atgaataatg gcttgtcacc atcagtcacg
    22921 ggttgattga tacctatatt ctcaccaatg tgtttctcag gtttatcgat gatatctgtg
    22981 attaatgctg cgacactctt acgtgatacg atcgtacctt tgaatggttc attgcgtgag
    23041 gtaagctcat aatcaataat atcttcatca gtcagccagg cagggcgaag aattgtatat
    23101 tccagtccag aagcctcaat tgcatccgca gccctgcgga atggttttag gggctcacca
    23161 ataactgcat tattccattc gccaaatttt cctggaactt catcataaat accgagggaa
    23221 agaacaaaga tcagacgttt aacatcacag gctttcatcg cagcgatcac tgaatttgcc
    23281 tgaatatcta aatcttcccc cgtaaggttg gcatacacaa cgtcctgtcc ctgcatggcc
    23341 tgcttcagtg ctgcatgatt cagtacatct cccataataa tcttgctatt agttggatag
    23401 ggtttgtgga tttttgctgg ctgacgggca aataaagttt gtttaatggt ttgtttatct
    23461 gccagttgat taatcacatg gcgggctatc tgaccgccag caccaagaat cagtacattt
    23521 ttcataatat ttctcatgaa acttatccca gatgggtgtt gaaaaaagga ataacttttt
    23581 ccagcgcctt ctgaactgct tccggtttgt catacagttc ataatgtgag aaatcgggta
    23641 atgaaactaa ctgacgatcc tgcgaaacca tcgcccggcc atagacttcc agtccatcac
    23701 gataagcacc gaaggcaccg actttttcac caactactac catcaccggc tgagtcagta
    23761 acacttcagt aaatgcgaag gcatcccagg ccagcgtttt ctgtgcatgt gagaacaaca
    23821 tttttgtcgc acctcccggt tgttgcccgc gtggtgtttt atagtagtca gttgcttcat
    23881 aaacatcgcg ttcggtcagt ccatgggctt ttgctgcatc caggctggcg ggaagtaatt
    23941 cgttgatctg taattctcca ccacgagcct ctttggttcg ttgcgacgcc attgcattca
    24001 atgcgccaat cggatcgtaa ttgctgaaac cttcccgaaa aagacgcccg atattaacac
    24061 ccgtaatact cactactgct ttaattcgtt tttctgtcag cgcggcatta atggcatagc
    24121 caccaccccc acacactccc agcacgccaa tacgttcagc attaacatat ggcagagtta
    24181 ctgcataatc gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccagc
    24241 gaggctcccc accagattct ccctggaaac tggcgtcata agcgagaacg agatagcctt
    24301 tttcagccaa tgctttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag
    24361 gatggacgct gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa
    24421 tattggcagc aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag
    24481 agtttcctca aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc
    24541 ataaagatcc atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc
    24601 tcgatcgtac aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg
    24661 cgtcggctga gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt
    24721 aaggctgcgc aaagtagcgt aacccggtgc tgttggatac tgcgcgcgag gggtgtggta
    24781 atactcccag gcctgacgca gttcttcgtt cggcgcatcg gactccttca ttggtgccag
    24841 tggcataatg gcgtattctc cgctgctaat atcgctggtt ctggcgtttg aacccgcttc
    24901 aacgtatggc agggcatcaa tagatttcac attgttttcc caaccattac ggaatatcga
    24961 accaatattg accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc
    25021 attggctgta tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac
    25081 ataagaaagc gttgtcaggt aatcaatcac ggcactgatg tcttcagtac gaatgtatgg
    25141 gttttctaac tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat
    25201 agtgacaaat cccttttccg ccagtttttt ggcataggtt ccggccgttt gttctttaac
    25261 gcccccacct gggtgagata ccacaattgc ctgatactga cgggtttcat caaattttgg
    25321 agggaaatag ctcactgcag acaaagagat ggttggatta ttgctgttag tgaagctgac
    25381 tttattattc atcattccgt tcctcatgga gttgtcggtt cgttttaacg gttggtgata
    25441 tcactataga tattgatcat taagttgatt agacccaaaa tcatgattag acctatcatt
    25501 taaatgatta atagattgca tggagatact gaatgaagcg tgaagaaatc gctgatctga
    25561 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg tgcagcagcc cgcctgagca
    25621 tggcgcagtc agctttaagc cagatagtgc gtcgtataga agaacgattg ggattgcggc
    25681 ttctgacgcg aaccacgcgc agcgttgttc caactgaagc gggcgagcat cttttgtctg
    25741 ttcttggccc tatgttgcat gacatagatt cagccctggc atccctgagc gagctgcaga
    25801 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
    25861 taccagcaat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag ctcaccattg
    25921 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtccgt ctgggtgggg
    25981 agatggataa agatatgatc gccattcgaa tcgggccaga tataccaatg gctattgttg
    26041 gctcaccgga ttatttttct cgccgaagtg ttccaacgtc agtgtcacaa ttaatagatc
    26101 atcaggcaat taatttgtat cttcccacat cgggtacagc aaatcgctgg agattaatac
    26161 gcggtggacg tgaagttcgt gttcgcatgg aaggtcagct tttactgaat acgatagacc
    26221 tgatcattga tgctgcaatt gatgggcatg gattggcgta tctaccttat gatcaggttg
    26281 agcgggctat taaagaaaaa aaactgatac gtgttttgga taaattcaca ccagatttac
    26341 ccggtcatca cctgtactat ccacaccgtc gacatgctgg ctcggcattc tcattattta
    26401 tagataggct gaagtataaa ggtgctgttt agcactactt gctgatacat taatttaatt
    26461 tctctcttaa cgtattctca gttcctttca acgttttggt tattttttat tcttcgtaca
    26521 atggcgacag atgctgatta tgataccgaa aactggtttg aacgtgcgaa gcctgaacgt
    26581 agtgttcggt gttctatgtg ctttaccgca ttttggcgtc gattatttac actaaatctg
    26641 atttaatata ttgatacata aaacatttta tgcttcctgt gtcacttttt tgatggaagt
    26701 tgtttgcatt tctttaaggc gaaacaaata attacgcatc aattttaatg tcggttagag
    26761 ggaaacttat gaagcactct gtttcagtca cgtgttgtgc gctgttggtc agcagcattt
    26821 ctctttcgta tgctgcagaa gttccgagcg gcacagtact ggcagagaag caggagctgg
    26881 tgcgccacat taaagatgag cctgcgtcgc tggatcccgc taaagccgtg ggcctgccag
    26941 agattcaggt cattcgcgat ctgtttgaag gactggtgaa tcagaacgag aaaggggaga
    27001 ttgtccccgg cgttgcgact cagtggaaaa gtaatgacaa ccgtatctgg acttttaccc
    27061 tgcgcgataa cgcaaaatgg gcggatggca caccggtaac ggcgcaagat tttgtctaca
    27121 gctggcaacg tctggtggac ccgaaaacgc tatcgccatt tgcatggttt gccgcgctgg
    27181 cgggaatcaa caacgcacag gcgattattg atggtaaagc tacgccagac cagcttggcg
    27241 tcaccgcagt tgatgcccat actttgaaaa ttcagcttga taaaccgttg ccgtggtttg
    27301 tgaatttaac cgctaacttt gccttcttcc cggtgcaaaa agccaacgta gaaagcggta
    27361 aagagtggac gaaacccgga aatctgatcg gcaatggcgc ttatgttctt aaagatcgcg
    27421 tagtcaatga aaaactggtc gtggtaccga atacccatta ttgggataac gccaaaacgg
    27481 tactgcaaaa agtgaccttc ctgccaatta atcaggaatc cgcagccact aagcgttacc
    27541 tcgcggggga tattgatatc accgaatcct tcccgaaaaa tatgtatcag aagctgttga
    27601 aggatattcc ggggcaggtt tatacgccgc cacagctcgg gacctattat tatgcgttta
    27661 acacgcaaaa agggccgacg gcagatcagc gcgttcgcct ggcattaagt atgacgatag
    27721 atcgccgcct gatgaccgaa aaagtgttgg gaacgggcga aaagccagcg tggcatttta
    27781 caccagatgt taccgccgga tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg
    27841 aagaactgaa tgcgcaggca aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac
    27901 cgctgaagct gacgcttttg tataacactt cagaaaacca tcaaaaaatt gcgattgctg
    27961 tagcatcgat gtggaaaaag aaccttggcg tagatgttaa attgcaaaat caggaatgga
    28021 aaacctatat cgatagccgt aacaccggca attttgatgt tatccgcgcc tcatgggtgg
    28081 gggattataa tgaaccctcc actttcctga cattattaac gtcaacgcat tcaggaaata
    28141 tttcacgctt taacaatccg gcatacgaca aagttctggc ccaggcatcg atggaaaata
    28201 ccgttaaagc gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg gagcaagcac
    28261 cgattgcacc aatttatcaa tataccaatg gacgattaat caagccgtgg ctgaaaggtt
    28321 atcccattaa taacccggaa gatgttgcgt acagccggac tatgtatatt gtgaagcatt
    28381 gatgtgatgg gaactggcgt tacccttgtg gataacgcca gtgatgtctg atttagcgcc
    28441 cctgttccgg cggtgtaatg ttatccatat acagcgtctg gctggggaag gcaaagtccg
    28501 cgccgtgtga ctgcacaata tcgataatct tcaaataaac gtcttgctgt gcagcaagcc
    28561 attcagccca taccgtggtt ttggtaaagc aataaaccat aatattcaat gacgagtcgg
    28621 caaactggtt gaaataaacc agtaaggttt gtcgctggtc gatggccggg tgatttttca
    28681 gcatctcacg tacggcttcg acaataacgc ccacttttgc cgcatcctca taacgtaaac
    28741 caatggtcgt ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg ctgatcgacg
    28801 aaaacagcga gttcggtacg tacaatggac gattatcaaa ggtcgtaatt ttggtaattc
    28861 gccagccaat ttccgctact gtaccttcga tatttctgtc cggtgaacgg atccagtcgc
    28921 caatactgaa aggacggtcg aaatagagca tgatcccgga aaagaaatta ctcagaatat
    28981 ctttaccggc cataccgaca gccagaccac caataccacc aaaggtcagc aagccagaaa
    29041 ggctcatgcc gaaatgttcg ccataaagca gaacaagcac cacaataatg gtgattttga
    29101 taatgcgcga cataatccgc gcactggtga tatcgcgacc ttttttaatc tgctgttttt
    29161 caaactgatt aatcagcaaa aatagcttaa tcgtcagaat aaccgcaatc agggacgtac
    29221 agataaaatc gataacgcct ggggtgataa atttgagttt atagttttct ataacataat
    29281 taataatgct accaacagca ctgataatta tggtgtagat taaaaattgc accgcatgga
    29341 ataaaaaacc ttttctttta cgatttccgc ggcgaaacca aaagctcatc agaatcaatg
    29401 ctgcgcagct accgaaaata atgaccagat taagcgcatt atttgtaaac agttcagcga
    29461 tcattgtttt atcaggctcc tccagataat tgtcgtcatg ccggaaaccc ctggcggggc
    29521 tattttaccg cgacaattca ttcagatcat caatagtcag ggaaggaagt agcaacatta
    29581 gctaactaat caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac
    29641 aacggtgaaa tgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga
    29701 ttacatcatg attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt
    29761 gttggcggtt ttcattattg cggcgtcggt tgtgactcgc gcgactatcg gcggcgttat
    29821 agaacagtac aatattccgc tgtctgagtg gacgacatca atgtatgtga ttcagtcatc
    29881 gatgattttt gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta
    29941 tttccttggc ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag
    30001 ttattgaaga gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg
    30061 tcaggtttaa tgaccaacag atcgcagcga agatgatcaa tcacctgttc cgccgtgttg
    30121 ccgaggaatg ctgctgaaat accggtgcgt cctaccgtgc ccagaaccac aatccccgcc
    30181 tgtaagtgct ccgccaaatc aggaatcacc tcttctggca gacctttttc tacgtgcgtc
    30241 atgttttcat taatgccgaa tttctgccgc agagctttca ttgccagcaa atgttgccca
    30301 cgaatggcat cgttataaac gctcggatca aattccggca gttcaatcgc gatattaatt
    30361 ggcgttaccg gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt
    30421 tcgatcgtct ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg
    30481 agattcaccg ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac
    30541 accgggcttg ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc
    30601 agacggtcat gttggtgcgc catttttagc accaaatcgt gcccgccgct gatcacttcc
    30661 tgaatgatgg cttcgaaggg acggttatgc cagaccactt taatttcaat gggaacgcca
    30721 gcattgagat aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc
    30781 tgacgcatag cggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca
    30841 tagatcggca aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc
    30901 cgcaatgctg gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata
    30961 gccatacagg gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca
    31021 tatctggtga atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa
    31081 tgatatgaca gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc
    31141 agcaagctgg gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag
    31201 cataccgctt ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag
    31261 atagttaccg atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg
    31321 ttgggcaaaa cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt
    31381 cttcgacaac agcaagatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat
    31441 catctgctga cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacgggat
    31501 ttcacatacc atcgaggttt ccagcgcctg cgcgaagctc gggtgatggc cgctgccgat
    31561 ggcgtcaaat cccaccaggt cgccagctaa atggaaacca gtgatttgct cgtcgccttg
    31621 ctcagtgatg gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc
    31681 atcaccagcc ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt
    31741 atcaagctga tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct
    31801 gcaatcctgg caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc
    31861 cgggatcata ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg
    31921 gagcaagtaa gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata
    31981 tgtctatcta tttgaaaacc cttaagttgt taagggtaac tttacataaa agtgtgaaca
    32041 agctggcaca aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac
    32101 tcttttcgct gaactccgcc tgcatatccg gtcatggtgc cgtttcgacc aataacccga
    32161 tggcaaggta cgacgatgct gatgggattc gatccatttg ccgcacctac ggcacgcgcc
    32221 gcgccagggc ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg
    32281 atagtgcgta gagttttcca gacttcgcgc tgaaatggcg tcccccctgt ggcagtggga
    32341 agcgtatcaa taatgctaag attaccggca aaataatcac gaagcttgtc gcttaaaccg
    32401 cctgggttgg tggcagaaat gcgctcatag ccttctttac gataatggat gtccagcagc
    32461 tgcaccatgc gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg
    32521 cacatcaccc acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc
    32581 cgttctctct taagacaaac gtgggtaaat ccccggacct atcggcagac caacaagata
    32641 ccacgccagc aacatcagca gccataccac caaaaagata agcggatagg gcaagactaa
    32701 cgaatagtaa gtacccagtt tcgcgtctgg tttgtagcgt tgcaggaatc caagaaacag
    32761 tggaacaaaa ggagataccg gcgctaaagg caatacggat gagtcggcaa tacgaaagag
    32821 gatttgcgca aatgccgggt gaaagccaag tagcataaac attggtacga aaatgggggc
    32881 cagaatcgac cagattgcgg aaccgctggc aataaacatg cataagaaag aggaaagcaa
    32941 cgccagaccg acaaacgccg ggatgccgct aagccctgaa ctttcaagta tatcggtcag
    33001 ccccacggcg atgaatttcc ccatgttgct ccagttaaac atggcgacaa actgggcgag
    33061 gggaaaaacc atcacgataa atcccgccat ctctttcatc ggttcaatca ttaaatgcgg
    33121 taaatccgcc tgacgtcgaa ttgtgcgggt agcgatgcca taagccagcg aaaccacaaa
    33181 gaaaaaaaga atgattagtg gcacgatacc tttaataaag ggtgatggca tcacggtgtg
    33241 attaatcgga tcgcgcaata tcccgttttc cgggatcacc atcagcgcga tcgcagcaat
    33301 aaaaagtagc gatacgacac ctgctatgcg taaaccaaaa cgctgacttt cggtcaatgt
    33361 ctgcagtttc tcatcgctgt ttccctgcca ttgacctaac cgtggctcga tgattttgtc
    33421 ggttatcaag ccgccaacaa tcgtcagtac gactacggag ctggccataa aataccagtt
    33481 atcaattaca ctgacgtgca tttgcggatt gaacgcagcc gccgcttccg tgctgatccc
    33541 cgacagcaac acgtcggttg tgacaatcag taaattagcc gtaaagccgc aacctacgcc
    33601 tgcaatggca gccagtaaac ctgcaactgg atgcctgccc accgccagaa aaatcagcgc
    33661 acccatcggt ggcatgatca ctaacgccgc atcggaagaa atgtggctga aaaaagcaat
    33721 aaacagcacc atataactgg cgtagcgggc attaacatgc gatgccattt taaccattag
    33781 cgctggcagt aagccgacgc gctccgctag gccggcacct aaaaccagcg ccaggatcgc
    33841 tccaagtgga gcaaaaccgc taaagttttt aatcacattg ggtaaaaacc agtgtaatcc
    33901 ttccacactg agcaggtttt tcaccaccac cggcgtacca tcggtcgggt ttttcgcact
    33961 gacgccaaag gctgacaaaa ttgccgtcgt caccatgagt acgattatca aatagataaa
    34021 gagcaaaaaa ggatggggaa ccttgttacc aattctttcg acccagccat agagcttccc
    34081 ggattgggag gacgacggta tggatgacat actcatgggc attcctcggt tgttgtgttt
    34141 gcgttgttgt tgttatttta aaggtgacgg tgtcacgttt ttcgggatag ggcagtgata
    34201 cggttgcgtg tccgtaactt gctgatgctc ttgttggcat tcttgcaata gccctgaatc
    34261 aatgaagaga ttgactgtgg ttgctgccat agttttcgcc gccagcagca ttcctttatg
    34321 agcaatagat gttcgcccct ggctaaccag ttgccacgta tgtagcggtg taccgacggc
    34381 aaaacagggg ctgaaacact gggcaacagg cagtttccaa ctgacgtcgc cgacatcagt
    34441 cgatgccgca agcacgttat cggtggcggc atatggagcg acctcattcg ccagtaccgt
    34501 ttcacgatga cgtagtgcaa aagccttgcc gttttcgcca ccggttgcgg cgatattatt
    34561 cagactgttt tgccgatcgt tgggggtgag cgtagcctga atttgtttcg caaaagccag
    34621 ttcttcggag ttccattccg gggtaccaaa atgggatagg gcatggtaca tggcattttc
    34681 taaggtgcga ttcgggagat aactggaaca ggctttgtcg aagcggcatt caaccgtggt
    34741 ttcggtcatc aatgccgcac cttcggcgat tttggcgacc cgatcataaa tatgctgcac
    34801 gtcggtcatt tcgggggcgc ggataagata aagcacttct gcctgcgcct ggaccacgtt
    34861 gggcgagatc ccgccgctat ttgtgatggc atagtgtacg cgcgcttttt caataatatg
    34921 ttcgttgagg aagttggtgc cagtggtcat caacgttacg gcatcaaggg cgctgcgtcc
    34981 caaatgaggg gaattcgcgg catgtgctgc gatcccttta aagcaccatg atgcctgaat
    35041 gtttgccagc gtgcgggtat tgaacatgcc ggcaaaggct tccgggtgcc aggtgagtgc
    35101 cgcatccaca tcatcaaata ccccctcgcg gaccatgaac gttttacccg agccgccttc
    35161 ttcgccagga caaccataaa agcgcactgt gccgccttgc ccatattgtt ccagccattt
    35221 cttgacggct attgcagcgg caaaggcggc ggttcccagc aaattgtgtc cgcaaccgtg
    35281 accattttca ccgggcgtcg cggatgtagg ttgcgcgcaa ccagcttgct gacttaaacc
    35341 tgccagggcg tcatattctc ccagcagggc gataaccggt ttgccttgac caaacgaagc
    35401 aataaatgca tttgggatat tgcctacgtt gcgggtaacg gtgaagcttg cagattccag
    35461 cgccgaagcc agatgctccg ctgaccagaa ctcttcaaaa cgtgtttctg gatgatccca
    35521 gatttgatcg gcaatatcgg tataacgttg gcgatcggct tcaatcgcat cgtcgataaa
    35581 acgatagatt tcctgcatca gatacctcgc gtccagggaa aattgagcgc ggtgcgcgcc
    35641 agcgtttcga cggcaatagc gagaacctgc tcgtcaaaat cgaatttttc gttgtgatga
    35701 cctgccgcca gctgtgtgcc aaacaccacg taggaggctt gcccttgatg gtgctgcacg
    35761 cgggccatca ttaatgtggc atcttcggaa cccgcaggcg cttcaacacg ttcaatggcc
    35821 tgattgaccc ccgcgacctg agccgcctga ctttgcaacc atgcgaccca ttgcggcgaa
    35881 ggagaactgg cggtagctgc acccatcagg cgagtttcaa cgccgacacc atacatggtt
    35941 gctgcgccct gaatcgcttg ttgtgcacgg tcaaaaacat attgattaat gacgtcgctg
    36001 gccccgcgtg tttccacttt cagcaacgcc gaggcaggaa caacgttacg gccgcttcct
    36061 gcctgcataa cgcccacgtt tactctggaa gctccttcgc tgtgcggggc gattgcatgc
    36121 agtgcaagag ttgcttgtgc tgccgccaac aaggcattgt gaccgtcttc tggttttgcg
    36181 cctgcgtgag cggcggtccc ggtgaagtgc gcgtcaaatt tggtggttgc cataaaatta
    36241 tcactgccgc acaccacggt gcccgcaggt acgccagtgc caatgtgcac ggcagtaaaa
    36301 taatcaacat catctacgac acctgcatcg accatcgccc gcgcgccacg cgtaccttcc
    36361 tctgcaggct gaaaaatcag tttgatgacg ccatgtagtc cggactcgaa ttgtttaagg
    36421 gtatgcgcca gcccaagccc aatggcggta tgcccatcat gaccacaggc atgcatcatt
    36481 ccggcgttac atgacgcaaa accgtcgcgg taggggcgat ggctgacatc ctgctcttca
    36541 ctgagatcca gcgcgtccat atcgacacgg aaagccataa ccggaccggg gcgaccggta
    36601 tccagggtgg cgacgatacc cgtgaaacca ccttcaaaaa ccgcaatcca ttgtgctagc
    36661 gcaccctgtt gacgagcgcg ctcgaattcg cgttgtagag tgagttcatc aggtagtccc
    36721 atccggctac tttcattaac tacttcgcgg cccagcgcca gtgaatagcc gagctgctgc
    36781 aattcttccg caacaagggt ggcagtgcgg aattccaccc agccagactc tgcatagtga
    36841 tgaaaatcac gtcgccagtg cgataatttt ggggcaagcg aattaacaaa ttgattcaaa
    36901 gactccataa cctttcccgt catcagtaaa aagtgtgacc cggttcacgt agcgatagtt
    36961 tttacttatc actaactgat ttttcacagt tttaaccgtt cataaattac cctgacacaa
    37021 tcatctgcat taaagtagat gccagtttct ttggtctgat aaataacggt tatcggtggc
    37081 gtcatggctt ttcaggtaaa aattcatcaa attcgggctt ttgttgaagt ggctcgtcag
    37141 ggcagcattc gcggagcgag ccgaatgttg aatatgtcgc aaccggcact gagtaaatct
    37201 attcaggagc tggaagaagg attagcggcg caactctttt ttcgccgtag taaaggcgtg
    37261 acgttaactg atgccggtga aagtttttat cagcacgcca gtcttattct tgaagagctg
    37321 cgcgcagcgc aagaggaaat tcgccaacga caagggcaac tggcagggca gattaatatc
    37381 ggcatggggg ccagtatttc ccgcagtctg atgccagctg tcatatctcg ttttcatcag
    37441 cagcatccgc aggtaaaagt acgcattatg gaagggcaac tggtgtcgat gattaatgaa
    37501 ttgcgtcagg gagaattgga tttcaccatc aatacctatt atcagggacc gtacgaccac
    37561 gaatttactt ttgagaaatt actggaaaag caattcgcga tcttttgccg cccgggacac
    37621 cccgccattg gtgcccgttc gatcaaacag ttactggatt acagctggac aatgccgacg
    37681 ccacacggca gttactacaa acagttgagt gaattgcttg acgatcaggc gcaaacgcca
    37741 caggtcggtg tagtctgcga gacgttctca gcctgtatca gtctggtggc aaaaagcgat
    37801 tttctcagca tactgcctga agaaatgggc tgcgatccct tgcacggaca ggggctggtg
    37861 atgttgccgg ttagcgaaat tttaccgaaa gcggcctatt atttgattca gcggcgtgat
    37921 agtcgccaga caccactgac cgcgtcatta atcacgcaat tccggcgaga atgcggctat
    37981 ctgcaaagtt aaaactgcat aaaaaaatag agtctgtcga catccgccag actctacagt
    38041 acacacagca gtgcatccgc gtcttaagtc cggcattgtc tcctctgcgc cggtgactgt
    38101 gttataacgg ttttaacctt tagttgccaa ttttcttcag tgacagattt cacgaaaata
    38161 taagttacat tattaatatc gtgaatgaat aatcatgcat aagcattttg cttaaaatat
    38221 cggcaatatt tggaacttat tactggaatt ttgggtaaga cgttgttgga ccgacccggt
    38281 ctggttatca tatcgcgctc ttaattgcgg gaggatgtaa catgaacctt gacgacaaat
    38341 cgctgtttct tgacgccatg gaagatgtcc agccgctgaa acgtgctacc gatgtccact
    38401 ggcatccaac gcgtaaccaa cgtgcgccgc agcgtatcga cacgctgcag cttgataatt
    38461 tcctcaccac cggatttctc gacatcatcc cactaagtca gccgctggag tttcggcggg
    38521 aagggttgca acatggggtg ctggataagc tgcgcagtgg taaatatccg caacaggcga
    38581 gcctgaatct tttgcgccag ccggtggaag agtgccgcaa aatgatgttc agtttcattc
    38641 aacaagccat ggctgatggt ttgcgtaacg tgctgattat tcatggcaaa ggacgggatg
    38701 ataaatcgca tgccaatatt gtccgcagct atgtggcgcg ctggctgacc gaatttgatg
    38761 atgttcaggc atattgcacc gcgctaccgc atcatggcgg cagcggggcg tgttacgtcg
    38821 cactacgtaa aacggcgcag gcgaagcaag aaaactggga gcgccacgct aagcgcagcc
    38881 gttgatctcg agacgcatcc gcggcttatg ccgccagcac gcggttgcgt ccatcatttt
    38941 tcgcccgata caaagcatca tcaacgcgtt taaacagttc atcgatgctt tcatttcctt
    39001 cgtgatgcgc cacaccaatg ctgacggtaa aacgtggtaa gcccgaaata ctcacttttg
    39061 ccacgcttac acggatagtt tcagccagcg aaagcgcggt atccagtggg gttcttggta
    39121 gcaataagac aaactcttcg cctccccaac gaaacaccaa atcgcctttg cgagcgcaac
    39181 tttcgagggt gcgggcgagg gcgcataaca cctcatcacc tttagaatgc ccatagagat
    39241 cgttaatgtg tttaaaacga tcggtgtcga tgagcaacaa gctgtaatcc tgggcgatgg
    39301 cgaggtgctg catttggcct ggctcggtaa tgtgataaaa ctgtcgccga ttcagtaatc
    39361 cggtcatcgc gtcatggtga gcagcatgtt ccagctgctc ctccagccgt ttttgctcag
    39421 taatatcatg cacaatacat aacatgagct tgtcgccata aatttcaatc ggcccggcat
    39481 aggtctgcac atgacgagta gatccatccg ccagtttatg aacaaaattc aaaggtttat
    39541 gaccgccggg taaatgcgaa atttcatgca tgataggcat gacgcgacgc ccgagcatat
    39601 taatttccca ggtatgtttc tggcacatcg tttcatggtt ataaccatag aaattgagcg
    39661 cggcgaggtt agcatcgacg atttggccat ctcgtgaggg gtcaatcaac aacattggtg
    39721 cagagttagt cagaaaaaag cgcgcataaa aaccttgttt tttgcgctga taatttgccg
    39781 agcgactggc ttttaaaccc agcgttgccg gcgcttcgat accttcgaaa ataatcaccg
    39841 gttctgtttc tgtcagctct cgcaaaacaa gccgacagct taatgctgtt tcctcttctt
    39901 tacgctgaac agtgaggatt tcgataatat cgtgttggtt ttgcagatcg gagaggtatt
    39961 tcggcagttc tttttgtgag gagacggaat agggtccggt tcgtagctga ctaaacgtga
    40021 ggtcttgcat caacagtcgc gccgcgctat tggcataaat taactgttcc tcaaagggcg
    40081 aaacaatcca gacaggactg gtgagtaagt ccagggtatt gaagttgtgc gtaatcattg
    40141 agatcccgtt atttttatca atttttgttg ctatccgatc gcaaaaaagc cacgtcatat
    40201 gatcagataa ttctgataat gatagacgct atttaacact tcacacggtt tgtatacgga
    40261 aaagcatttt gctttttgta ttcaatttag acagaatttt attaatcatt tcagggtaat
    40321 ggggtgatga gatgttgcgt aacagggcca gaaggctaga ctacaaaata atgcgttgat
    40381 gatggaggca ctgtggaagc gattaaggga tcggacgtta atgtcccgga tgcagtattt
    40441 gcctggatgc tggatggtag aggcggcgtt aaaccgctgg aaaatacaga tgtgattgat
    40501 gaagcgcatc cctgctggct ccaccttaat tatgtacacc atgatagcgc ccaatggctg
    40561 gcgacaacac cgctgcttcc caataacgta cgtgatgcgc tggcgggcga gagcacacgg
    40621 ccccgagtca gccgtctcgg tgaaggcacg cttattacat tgcgctgtat aaacggcagc
    40681 accgatgaac gccccgatca actggtcgcc atgcgtgtat atatggacgg gcggttaatt
    40741 gtttcgaccc gacaacgcaa agtgctggcg ctggacgatg tggtgagcga tctggaagag
    40801 ggcacgggtc cgaccgattg cggagggtgg ctggtggatg tgtgcgatgc gttgaccgat
    40861 cattccagcg aatttatcga gcagctgcac gataaaatta tcgaccttga agataatctc
    40921 cttgatcagc aaattccacc gcgtggattc ctggctctgc tgcgcaaaca attaatcgtg
    40981 atgcgtcgct atatggcacc gcaacgtgat gtctatgctc gtcttgccag tgaacgtttg
    41041 ccgtggatga gcgatgacca acgccgtcgg atgcaggata ttgccgatcg ccttgggcgc
    41101 ggccttgacg aaatcgacgc ctgtatagca cggactggtg tgatggcgga tgaaatcgct
    41161 caggtgatgc aggaaaattt agctcgtcgt acctatacaa tgtcattgat ggcaatggtc
    41221 tttttaccca gtacctttct gacagggtta tttggcgtca accttggtgg gatccctggc
    41281 ggcgggtggc aattcggatt ttcaattttt tgtattctgt tagttgttct tattggtggt
    41341 gttgctttat ggttgcaccg tagtaaatgg ttgtaacaaa agcaattttt ccggctgtct
    41401 gtatacaaaa acgccgcaaa gtttgagcga agtcaataaa ctctctaccc attcagggca
    41461 atatctccat tgcaggtgaa tgcaacgtca agcgatgggc gttgcgctcc atattgtctt
    41521 acttcctttt ttgaattact gcatagcaca attgattcgt acgacgccga ctttgatgag
    41581 tcggcttttt tttgcctgtt atttatcagc gtctaccctt taagagtcca cccaatgacc
    41641 ggagggaaat atgacgacac ttatttattt gcaaattcct gtccctgaac cgattcctag
    41701 cgatcctgtt ccagtgcccg atccgatccc tcgcccgcaa cccatgcctg acccaccacc
    41761 cgatgaagaa ccgattaaat tgtcgcatcg tgagcgtaga tctgcgagga tacgcgcctg
    41821 ctaactttgc gtcgatgacc acgagaatag attgtgaccg ctttttctac cctgaatgtt
    41881 ttgcctcccg cccaactcac gaaccttaat gagttgggtt atttaaccat gacgccggtg
    41941 caggccgccg cgcttccggc gatccttgcc ggaaaagatg ttcgcgtgca ggcgaaaacc
    42001 ggcagcggca aaacggcggc ttttggcctc ggcttgttac agcaaattga tgcgtcgcta
    42061 tttcaaaccc aggctttagt gctgtgtcct acgcgtgaac tggcggatca ggtggcaggt
    42121 gaattgcgtc ggctggcgcg ttttctgcca aataccaaaa ttttgacgtt gtgcggtggt
    42181 caaccgttcg gtatgcagcg tgattcgttg caacatgcgc cgcatattat cgtggcaacg
    42241 ccgggtcgtt tgctggatca cttgcaaaaa ggcacggtat cactggatgc gctgaatact
    42301 ttggtgatgg atgaggccga ccgcatgctg gatatgggat ttagcgatgc cattgatgat
    42361 gtcatccgtt ttgcgcctgc atctcgacag acgcttctgt tttcggcaac ctggccggaa
    42421 gccatcgccg caatcagcgg acgagtacaa cgcgatcctt tggcgattga aattgactca
    42481 acagatgctt tgccacccat tgaacaacaa ttttatgaga catccagcaa aggcaaaatc
    42541 cctctgttgc aacggttatt aagcttgcat cagccatcct cttgcgtggt gttttgcaat
    42601 accaaaaaag attgccaggc tgtctgcgac gcgctgaatg aagtagggca aagtgcattg
    42661 tcgttacacg gcgatctgga gcaacgcgat cgcgatcaga ctctggtacg ttttgctaac
    42721 ggtagcgccc gtgtactggt tgctactgat gttgctgcgc gtggtctgga tattaaatcg
    42781 cttgagctgg tggtgaactt tgagctggcg tgggaccctg aagttcatgt acatcgcatc
    42841 ggtcgtacag ctcgtgcagg aaatagcggt ctggcgatca gtttctgtgc tccggaagaa
    42901 gcacagcggg ccaatatcat ttctgacatg ttgcagataa aacttaactg gcaaacgccg
    42961 ccagctaata gttccattgt gccgctggaa gcagaaatgg caacgttgtg tatcgatggc
    43021 gggaaaaaag ccaaaatgcg cccgggtgat gtattaggtg cactgacagg agatatcggg
    43081 cttgatggcg cagatattgg caaaatcgcc gtgcatccgg cgcatgtcta tgtcgcggtc
    43141 cgtcaggctg ttgctcataa agcatggaaa cagttacagg gcgggaagat taaaggaaaa
    43201 acgtgccggg tgcggttatt aaaataatga aatgttgaat tgccgggtgc aagagtaaac
    43261 atcttattcg ggattgccgg atgcgacgct ggccgcgtct tatccggcct ccataagagt
    43321 agcccgatac gcttgcgcat cgggcgctat cctggttatt tcacttcaac cacattcagt
    43381 cgtaactcat ccaactgatt ttcatcttct tctggctgcc agcccgccgg ttgtagtggg
    43441 atctcttcgc gatcaaacgc cagatcaccc ccgttaacca cttcagaacc gtgggtaatg
    43501 cctttaaaat cgaacaggtt ggtatcgcac agatgcgacg gcaccacatt ctgcatcgcg
    43561 ctgaacatcg tctcgatacg ccctggataa cgtttatccc agtcacgcaa catgtcagca
    43621 atcacctgac gttgcaggtt aggctgtgaa ccgcacaggt tgcacggaat aatcgggaac
    43681 gcttttgcat cggcaaatcg ctgaatatct ttctcgcggc agtaggccag cggacgaata
    43741 acgatatgtt tgccatcatc gctcatcagt ttcggaggca tacctttcat cttgccgccg
    43801 tagaacatat ttaagaacaa cgtttgcagg atatcgtcac gatggtgacc caacgcgatc
    43861 ttcgtcgccc ccagttccgt tgcggtacga taaaggatac cgcgacgaag gcgagaacac
    43921 agtgagcaag tggttttgcc ctctggaatc ttctctttca cgataccgta agtattctct
    43981 tcaacaatct tgtactcaac gcccagcttt tcaagatact cgggcagaac gtgttccggg
    44041 aagcccggtt gcttttgatc gaggttaaca gccaccagcg aaaaattgat tggcgcgctt
    44101 tgctgcaaat tgcgcagaat ctccagcatg gtatagctgt ctttaccccc ggagaggcaa
    44161 accatgatgc gatcgccttc ttcaatcata ttgaagtcag caatggcttc gcccacgtta
    44221 cgacgcagac gtttttgtaa tttgttcagg ttgtattgtt ctttctttgt aatttgttga
    44281 ttttcttgca ttatttcagt tctctggtac taaatggggc aaattggggg caaactttgc
    44341 aactatgata accgcgcatt caacatggct atctgttcgt cgttcatgtc atcaatccac
    44401 ataccgtaaa tttcatacac catctgcgca gtttcatgcc ccatttggct ggctataaat
    44461 gccgggttcg ctcctgccgt caacagccag caggcaaaag tatgccgcgt atggtacgga
    44521 ttacggcggc gaataccagc acgttttact gctgcattcc accttgcccc caaactgctt
    44581 accgagtaat aaggtttttg ttttccgtta cacaccctgg gcatgaaaac aaaatgcagt
    44641 ttttgctttt cggttctgcc gtactcccga tgataaaagg tgatttcgct tttgcgatga
    44701 tgcccggtca gtttgtattg ctccttcagt gcttcaagag caggctgcag tagtgttact
    44761 gtccggatcc cggcatttgt ttttggggga ccgaacatat caagtatcgt caggtttctt
    44821 ctgacattca ctattccctt ttcgagatcc acatcctccc acgccagagc tgccagttcc
    44881 ccgtgacgaa gtcctgagta aactgcaaat ttccacatgt tctggctctg tcctttttca
    44941 ctttccatta atgcattgaa ttctgtttta gataacggat caggctttat tctgtttcgc
    45001 tgtaattttt ttactccttc aaatggtttg gttgatataa atcccgactg atacgcaaaa
    45061 cgtaacagcg aacagagcag ggcgatatag ttatcaactg tgcgcacggt tcttcctttt
    45121 ttgttggatc ttggattatc caggtaaagc gtttctccat gcagcagttc attccggtag
    45181 tttaagatat cgctataacg aatatgtgat atcagggtac tttcacaaat tattattctg
    45241 agtgttttta attgtgattt cgttttcttc attgtgtttg ttgttaactc tgtctcttta
    45301 atttttgtcc agatatcaca aagctctccg aacgttttta tgactctcgt tgtcaccatt
    45361 tttgccccag tgctggactg gggaaaacgt cttaaatact caaattcacc ggagtttatt
    45421 tcatgaacta tcagcgctct taaatttccg gcctttttaa tattactgtt tgtaatctcc
    45481 cagcctttta atgtttcccg acatcgtttt cctcgaaaca tgaaccagat gcgaatgtat
    45541 ctacctctaa tctcgacacc tgttggtaat ttagacatat catgagtctt tgataaactg
    45601 atttatcttt ggatagttgt accagataat ccctcgtttg ctgtctggct tacctaaagg
    45661 agatactcgt ttgaagtgga agccctccac ccaacagttc tggcggtatg cttcaatttg
    45721 tctggccccc agaccagtgc gaagcatcag gccgtattca accatccact cttcattaaa
    45781 gattacttgt gccatcgcat cacctctggc aggcgccaat gttagactga aattgacgcc
    45841 cgatgttgat tattaataat cagctatgaa gttttaattt gaatacaatg caattcacga
    45901 ggactgaagt ttctcgcaat taaaatttat cagttttact ttctgctctc tggaaacgcc
    45961 tgcttctttt ttacctgaga gcattttttc gcattctgat ttcgttagtt tagattttga
    46021 atatcttgtc cagttagtag gagtgccacc ttccttttca atagtggcgg taattttata
    46081 catgaacacc tccattatta tttccagtgg ttcgtttatt tcatcgttcg agtgcttctt
    46141 tttcacttcc accataaccg gttcgggatt cgcatccgtt acacttcgct cggtaatatc
    46201 ctgaaatggc tttcaccgtt actgatggac aaccacaaaa tggacatggt ttaacattgt
    46261 catatctcat aatttttctc ataaaaactg tctcaagttg gcggtgcatt acaccgccag
    46321 gctgaattat tcctctgaat tatcgattac actgtattcc ccggttaata cagaggaatc
    46381 tgcaggatcg attgtcagtg gttccttttc atccattgat actgcacgct ggatctcaat
    46441 tgatacgggc agatatttga acaggcgacg aatagccgtt ttctttgcca tttcttccca
    46501 gtgagttacc cacggcccgt tattaccagc tttactctgg ctgcgcacca gctcaatctg
    46561 tttgcgcgtc ataacttcaa actgagtacc tccgtctttc agtcttgcga cagcatagac
    46621 gtgggtaaca ggggcatctt cgttttctcc tgggcggtgt attaactttt catcaaggcc
    46681 aaattcgaaa ttaaactcgt caccttcacg gacaacacgg gctgacaggc tggcgatttg
    46741 acctgaacgg cgagccagat caatcatgcc gcgatagcca atgattagct gaacgttttt
    46801 tttaccgctc ttttcgtttt tattaccaaa aggcagtaaa tatgcatgac cgagggcgct
    46861 accgggctca agtccgagct gtgaacactg tacgattgca ctgacaaaac tcatagtgtc
    46921 acagtttcct aacgccggaa ctttacgaat ttctgtggtg gcgatacgga tcatacgttc
    46981 agccgtcata tggcgtggaa gagctgctgc cagttgctct ttcattgatg gctggttaat
    47041 aaaactaatc acgtcgttat ttttaattgc tgctggtgca cggtttccct gagttttttg
    47101 cagatcggct tttgcgattg gtggttgctt agtcatttgc atattcctta gcccagcggg
    47161 gcagtgataa cgtcttaata gctggccatt catcggtatt gaggcagtca gccagggttc
    47221 gcagattgcg gtgatattcc agctgacctg ccagttttgc ttcttcgccc atcatgaaaa
    47281 tttcaaccgg ataacgtccg cattcaatag ttgtgctggc aaccagaaaa acgaaagttg
    47341 gctgcactcc aaactgtgct tcataaccgt cactgtagaa tgcatcctga acgtgatagc
    47401 ggtagtcgta ataagcggtt ttgaatcgtt gaatatccgc cgtagttttc acgtccatga
    47461 tccagtgaaa ttcagggata attttgtccg gacggcaccg acacaaaatt gctgtttcag
    47521 gatcttccca gtaaattgat gattcagcgt gtccggcgct ttcaacaagc cattgcccca
    47581 gcggcaaagc cataacgctc tgatacatga gttcaatttt ccggccttct tcggcagtga
    47641 taaccgtttt tcctgtgctt gcgcattccc tcagaaacgc tttctcttct tcttttccgg
    47701 agtttgtccg gcggttaaat tcaggtgcta cgataaagcg gttactgaat tcttccggct
    47761 caagtacccg gcagtggaaa gcggttccta aatcgagcgt ttttgtcttt gtggtgtcca
    47821 cgggggcatt tttacgccac aaatacagtg ccggagtatc agcaatgtca tcgagctgag
    47881 acttactgac accgggaccc gcgtggtaat tctcattcga aattccgtaa taaatacctg
    47941 gctctatgtc ttctacgatt acgggatctg cgacttcgcc agtttcatca ctgcaatcgc
    48001 gatgcggatc gctgccagca ttctcattgt gcggatgttc agcgccttcc atttcctccg
    48061 gatcattttc cttagcttca acctgactct cttcatcgaa tgtttcctgg tatgttgcgt
    48121 cgcccatcac cgcaccacag tcagggcagt tatccccgcc agtctggccg caggcattgc
    48181 aggctatttc cggttcctgt tgcactactg gctcaggttg attcatatcc gggctggttt
    48241 tttccgtttc tggctggttc tggtacacac aatcgcgagt ctggatcccc tttacccatt
    48301 tcggatcgtt cgggtcgcta attccgtcaa caaattcacc acgtgatgca gcaagcaatt
    48361 tatcggcatc gacaggattt tttgatggaa tgtttttccg ggcttcatgg agttctgccc
    48421 gcagttcctg atatttcgca tcaacagaat ttacctgtga ctgagcatcc agcggctgcg
    48481 tgtcctgatg atgttcagtt gcgtccggtt ccattgtttc agcctctccc tgttcaactg
    48541 ccgttgttcc agatggttgc ggtttttctt cataatcctg ttttccttct tctgttactc
    48601 gctgcggcat cggggcagag gagcgaccgc aggcaatatc cacgatttcc ggatcagggt
    48661 tggcatgatc ggtttcagtc agtactttgt tcagatattc agtgacgtgc gcggggatga
    48721 cctcgatccc aattggtgct tcttttacgg acgcaaccac gatggcgcgg gaataatcca
    48781 gcccgccagg catggtgatg aatttgtcgc ggaaaacaga aaagggcggt ttattttcag
    48841 cgataatttc ctcaatgcgt ttagcgtgtg ccggatgaag gttatagatg tccagatcca
    48901 ttgaacgggc cagtacgcca gtggctacgt cccgcgccag tgacgtcaga tcgtgtacga
    48961 aaccttcgcc gcgatcggtg aggtttccgc cgccagcatt agcaccggaa gccgtgcgag
    49021 tgatgtgtga aacacgatta cccttcatcc actcttttgt cagcagtcct cgatcggtgt
    49081 agtcagcgtt caggtatgct tcgaaaaaag cagttatcag tcccaggttt gaattaccag
    49141 gattagggaa aactttgtca gtgtcacgaa ccagtttgtg gagttcgcga atttccagcg
    49201 ggtcgagcag gctggttttg tgggaaacag ccagggcagt aacagccggt agttcttcag
    49261 cccgagcaat gtgtaatgcc tggagtccgt cgcgtgaaac gtgcgttacc ggtttttcgc
    49321 tgccgtgttg agcaagccaa cgaatgggca gttcctggcc agaaattggg agtagcatat
    49381 tctcctcaat ctcagtcatg tcttcgccgt tgacgttggt attgccttga tagtgagcgt
    49441 tgtctggtgc tgctcccggt tttagttccc atgtcatgga gtctttgctg agttgatagc
    49501 gttcactcca ggtaaaatcg atctcacctt cagcgggcag gtcattaacg acaggaaaat
    49561 tcgtggcaac agctttaaaa tagctgctca gttttttacc tgacttaacg atcaggtagt
    49621 ccagagtggc acaggtcgat tcaaaatcgt tgcttgccca caggacgacg tcaggttcac
    49681 cggatgattt tttcgctttc cgtaacagga agagtggttt tgtgctcatt gttttttaac
    49741 ctcaactcag attaaaattc gttttgttca gtgaatgatc ttgccggata cacactgttc
    49801 atagcctgcg ccatacgcag gctatttctt tcagatttca ccttttaatt tcattgcaat
    49861 tagagttgcc agaaattcgg cttttttttc tgcgggcaga ttctttccga tatgcaccag
    49921 gcacattttt ttgacacctt catcaagtgt ttttacgttg cctgatggac catcgatatc
    49981 aaccacagtg aatggggttt ctttattttc tgttttaatt acgtagccaa tgcgctttcc
    50041 ttccagattc acctcgtgaa caatgtcatc ggtagttaca acagtggctt cataattggt
    50101 aatcatgttt ttctccttaa ttaaggttga gcgaatacct gccatttctg gcataaattc
    50161 agtttcgaat agtcaattaa ttaaagttca tgtgccatct ggtctttttc ggcacaagct
    50221 tcactgcaat attttctcgg ttcgtctttt gataaaatcc cgtgcatgaa gtgaagcatt
    50281 ctttcaatag ctttgctttc ttcaacgtct tttttgcaaa ggtggtaagc acattttatt
    50341 ttcttagtca tcaccatgac tccgccttta caggtaaacc atcacgaccg aggaagactt
    50401 taatcatgcg gtcagtaatg catgtttttg tggtcaggtt acgaatatat agttttcgct
    50461 ttttaatatt gtttgccgag gcaatatatg tccggccttc atgaagaaca taatcgccag
    50521 gagtcacaca ctgacgtggt atttcatcag ttccgaagtg atgtgcaatc ataattatct
    50581 ccatttttac aaatgaactt tgttgatgcg gtgcctggtg cctccaggtg actgcaacca
    50641 gttaacaatt acagtcggct ttcccaccca aaccaataag gactaacatg acttttaact
    50701 gtgccgcgtg cgcttagccg cattcaccgc atcacaaaat tcactttaaa aagggcggac
    50761 atcagtcgaa cttcaagaaa aaactgatgc cgccaagact acacacagca gtgttgttat
    50821 tcacaaccgg aggcgcactc ccaccattta aatttaacag acaagaccga ctctttatgg
    50881 ataccggaaa tgcgccttcg tgttgtgccc ggttttattt caccacctcc gggctttggt
    50941 ggcctcggct atacccctac agcaagaata ttgaattaat ccaataaatg gtttagctgg
    51001 tatttttggc aagccagcga cgggcaccat tttcggtttt aaacgttttg cttttggtat
    51061 acgtcattgc ggtgaacgtg ccgtcctggt ttggaaacac gccgtacacc agagatgcgt
    51121 tgttgccaag atcgatagta tccatgctga cctcatttcc ccttaacgcc ggggtagcgg
    51181 aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc atacgcctcg
    51241 ggctggctac ttaacccctg accactgccg ggtaactcga agtattgccc tgcgttctgt
    51301 ggggcggggt gggttggtag gtatataatg tactttgtgt tcattgatgt aaagtacttt
    51361 aagtacattt tgtgtgtaaa aaaatgagat gggataaagt gaagcacaaa cccggagggg
    51421 gacgctaccg gatttatgct ggtttaagag gctttttgtt ttttctttcg tgctagctct
    51481 tcgtaaattg cattgtactt ctgttttttc tcctcaagag tttttaaaag ttcatctgtc
    51541 tcactgtcag ggagctcgtc cagaaggtca atgatgattt tttgtcttgg atttaactcc
    51601 tgatagaaac gtacctgtcc actttcttct gtatcctctc ccaaaagata ggttggtgtt
    51661 gttccaatga gtgttgctaa ttcccttaat ttctctcggc gaggaattgt ttcgccatta
    51721 aaccatttgc taaccgcttt tggtgttaat ttcattcgac gggcaatttc tgcctgcctt
    51781 ccatgttgtt cataaccagc gttttcacag gctagcgcaa gcctactggc gaactcttta
    51841 cgcgctttat cttcatgaac cataagttca atgatattcg ctcttgaatg tactgtcagt
    51901 tctgttatag catgtactca aagttcacat tgtgagggtg atatgaacca gaaaacactt
    51961 gaagatgtaa tcaaaactgt tcgcgtttct gttgtggccg acgtttgtgg tgtcagccaa
    52021 agagcaatct acaaatggat ggataacgga aaattgcctc gcacagaata taccggcgaa
    52081 acaaattacg ctgaaaaaat cgctcatgca tcaaacggat tattttctgc tgatgtaatt
    52141 ttaactattg gcagaaataa aactactacg aaaaagctga tgggagttga ttcatgaaaa
    52201 tcaagcatga gcacatcgag tcagtgttgt tagccctggc agccgaaaaa gggcaggcat
    52261 gggtagccaa tgccattact gaagaatatc tgcgccaggg gggcggcgaa ttgcccctgg
    52321 taccaggcaa ggactggaat aatcagcaga atatctatca ccgttggttg aaaggtgaaa
    52381 cgaaaacgca aagagaaaaa attcagaagc tgatcccagc aattctggca atccttccgc
    52441 gcgagctgcg tcaccgactc tgcatcttcg ataccctgga acgccgtgca ttactggcgg
    52501 cgcaggaagc gttaagtacg gcaattgatg cgcatgatga tgcagtccaa gccgtttacc
    52561 ggaaagcgca tttcagcggc ggcgggtctt ccgacgattc tgtcattgtt cattaagcaa
    52621 aagtttccat gctgtttgtg cttattctaa gccaccgggc agcatcatac ggggcaatta
    52681 tggccgcatt accatacatg caactgtaca tagctgatta cctggctgac accatgcatt
    52741 tgtcagcaga ggagcatggt gcgtatttgt tgctgatgtt caattactgg caaacaggaa
    52801 agccaatacc taaaaacagg ctggcaaaaa ttgcccgtct gactaacgag cgatgggctg
    52861 atgttgaacc atccttgcag gagttttttt gcgataacgg cgaggaatgg gtgcatcttc
    52921 ggattgagga agatctggca tcagtcaggg aaaaattaac caaaaaatca gccgcaggaa
    52981 aagcatctgt tcaggccaga agaagcagaa aggaagcaga tgttcaaaca aaacaagaga
    53041 gaaatttaac aggtgttcaa acagatgttg aagtggtgtt tgaacatgat gtcaacacaa
    53101 aggcaactaa taaagataca gataaagatc taaaaacaga tcccccccta aatccccccc
    53161 gggggaatcg aggtgtcaaa aagtttgacc ctctggatat tgctttgccg aactggattt
    53221 ctgtctcgct ttggcgtgag tgggttgaat ttcgccaggc attgcgaaaa ccgattcgaa
    53281 cggagcaggg cgctaacggg gcgatacggg agctggaaaa attccgccag cagggttttt
    53341 cacctgagca ggtgattcga cacagcatcg ccaatgaata ccagggcttg ttcgcgccga
    53401 aaggtgttcg acctgagacg ttactccgac aggttaacac cgtctcgtta ccggatagtg
    53461 cgatcccgcc aggcttcagg gggtaactga ccatgaaaaa tattgcgaca ggcgacgttc
    53521 ttgaacgtat ccgcagactg gccccgtcac atgtaaccgc gccattcaag acggtagcgg
    53581 agtggcgcga gtggcaactt tccgaaggcc agaaacgttg tgaggagatc aaccgtcaga
    53641 atcgtcagtt gcgggtggaa aaaattctga atcgctctgg catccagcca ttgcaccgca
    53701 aatgctcgtt ttcgaattac caggtgcaga acgaagcgca gcgatacgcg ttgagtcagg
    53761 cgaaatccat cgctgatgaa ctgatgaccg ggtgtacaaa ttttgcgttc agcggaaaac
    53821 ctggtaccgg gaagaaccac ttagcggcag ctatcgggaa tcgcctgctg aaagacggtc
    53881 agacagtgat tgtggttacc gtggctgatg ttatgagtgc cctgcacgcc agctatgacg
    53941 atgggcagtc aggcgaaaaa tttttgcggg aactatgcga agtggatctg ctggttcttg
    54001 atgaaattgg cattcagcgc gagacgaaaa acgagcaggt ggtgctgcac cagattgttg
    54061 atcgccggac agcgtcgatg cgcagcgtgg ggatgctgac aaacctgaac tatgaggcca
    54121 tgaaaacatt gctcggcgag cggattatgg atcgcatgac catgaacggc gggcgatggg
    54181 tgaattttaa ctgggagagc tggcgtccga atgtcgtcca gccaggaatt gagaagtaat
    54241 ttttaccggg aggaaatttt tatggagact gtttttgacg cactgaaagc aatgggaaaa
    54301 gccacatcca tagaacttgc tgcgcgactt gatatcagtc gtgaagaagt gctgaacgaa
    54361 ctatgggaac tgaaaaaagc tggtttcgtt gataaaagcg cgtacacctg gcgtgtggct
    54421 gataacaacg ttcagcagga acagccaatg caggcagaac tgccggaaga aaccaccacg
    54481 gcaactgtag tgaaaatctc agagtgcgat ttaaccgcga cgattgaaca acgcggacca
    54541 caaacggcgg atgaactggc tacgctgttc ggtacaacat cccgcaaagt tgcttcaacg
    54601 ctggcaatgg caatcagcaa aggacgcctg attcgcgtaa accagaacgg taaatttcgt
    54661 tactgcatgc cgggcgataa tttaccagca gagccgaaag ctgcatcggt agcggaaact
    54721 gatggtaaag cctttcctca gccagccggt gttgcgttac cagtacagga agcggcaaca
    54781 caggaagata ttaaaacaga aactgtggcg gacattgtgc agtcgctgcc atcgtttact
    54841 gaaacgcaag cggatgacct agttttacca tcgctgcata tggcaaaccg cgaactgcgt
    54901 cgggcgaaaa atcatgtcca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
    54961 aagcaccggg atattgtccg acagattgtc gattcctcca gtcgtattgt gtcggaaaag
    55021 tgattgccgg aggcgcttat ggcaaaagtt tttacacagg aagagcgtga aaaaattaag
    55081 gagcaggttg ttgagttggt acgccagagt gggcgcgaga cgttaaggca actggaggcc
    55141 aagacaggtg cgacaagata tctgatgagc attcttgcca gagagttggt tgccagtggc
    55201 gatgtataca actctggcta tgggttattc ccgtctgaac aggctcgtaa ggattggaaa
    55261 aacgcccgca aaaaactatc cagggcaaag gtgaaaaaaa cggttgtggt tgatcctgac
    55321 cttatctggt cattaccaga cggagaaata cgccgctatg acaggcgtca gaacataatt
    55381 tgtcgtgaga gcaggaggag cgaagttatg cagcgtgtgc tggcgttcta tcggggaaac
    55441 tttcaggagg tgatggagtg agggtgagag tttatattgc cggtccaatg acgggatatg
    55501 aaaatttcaa ccgtgaggca tttcacaagg tggaagagga actgaaacgg gaagggcata
    55561 ccgtcttaaa cccggcagta cttccggacg ggctgacaca gccgcactac atggatattt
    55621 gcatggcaat gattcgttgt gtggatgcga tttacatgct gaatggctgg cagcggtcag
    55681 cgggcgctaa ggcagagctg gcactggcgg agaaactggg gcatgcggtg atttatcagg
    55741 aggtggctc
//
LOCUS       DAZKGS010000011        55414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKGS010000011 DAZKGS010000000
VERSION     DAZKGS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..1512
                     /gene="yajR"
                     /locus_tag="ACHOQY_000703"
     CDS             148..1512
                     /gene="yajR"
                     /locus_tag="ACHOQY_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9676853.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1640..2131)
                     /gene="yajQ"
                     /locus_tag="ACHOQY_000704"
     CDS             complement(1640..2131)
                     /gene="yajQ"
                     /locus_tag="ACHOQY_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9676854.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2299..3210
                     /gene="panE"
                     /locus_tag="ACHOQY_000705"
     CDS             2299..3210
                     /gene="panE"
                     /locus_tag="ACHOQY_000705"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9676855.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3173..3763
                     /gene="yajL"
                     /locus_tag="ACHOQY_000706"
     CDS             3173..3763
                     /gene="yajL"
                     /locus_tag="ACHOQY_000706"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9676856.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3817..5265)
                     /gene="thiI"
                     /locus_tag="ACHOQY_000707"
     CDS             complement(3817..5265)
                     /gene="thiI"
                     /locus_tag="ACHOQY_000707"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9676857.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5471..5713
                     /gene="xseB"
                     /locus_tag="ACHOQY_000708"
     CDS             5471..5713
                     /gene="xseB"
                     /locus_tag="ACHOQY_000708"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9676858.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5713..6612
                     /gene="ispA"
                     /locus_tag="ACHOQY_000709"
     CDS             5713..6612
                     /gene="ispA"
                     /locus_tag="ACHOQY_000709"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9676859.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6637..8499
                     /gene="dxs"
                     /locus_tag="ACHOQY_000710"
     CDS             6637..8499
                     /gene="dxs"
                     /locus_tag="ACHOQY_000710"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9676860.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGMELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8679..9653
                     /gene="yajO"
                     /locus_tag="ACHOQY_000711"
     CDS             8679..9653
                     /gene="yajO"
                     /locus_tag="ACHOQY_000711"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9676861.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9707..10225)
                     /gene="pgpA"
                     /locus_tag="ACHOQY_000712"
     CDS             complement(9707..10225)
                     /gene="pgpA"
                     /locus_tag="ACHOQY_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9676862.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10203..11180)
                     /gene="thiL"
                     /locus_tag="ACHOQY_000713"
     CDS             complement(10203..11180)
                     /gene="thiL"
                     /locus_tag="ACHOQY_000713"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9676863.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11258..11677)
                     /gene="nusB"
                     /locus_tag="ACHOQY_000714"
     CDS             complement(11258..11677)
                     /gene="nusB"
                     /locus_tag="ACHOQY_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9676864.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11697..12167)
                     /gene="ribE"
                     /locus_tag="ACHOQY_000715"
     CDS             complement(11697..12167)
                     /gene="ribE"
                     /locus_tag="ACHOQY_000715"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9676865.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12256..13359)
                     /gene="ribD"
                     /locus_tag="ACHOQY_000716"
     CDS             complement(12256..13359)
                     /gene="ribD"
                     /locus_tag="ACHOQY_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9676866.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13363..13812)
                     /gene="nrdR"
                     /locus_tag="ACHOQY_000717"
     CDS             complement(13363..13812)
                     /gene="nrdR"
                     /locus_tag="ACHOQY_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9676867.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13963..14502
                     /gene="yajI"
                     /locus_tag="ACHOQY_000718"
     CDS             13963..14502
                     /gene="yajI"
                     /locus_tag="ACHOQY_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9676868.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14801..15679
                     /gene="tsx"
                     /locus_tag="ACHOQY_000719"
     CDS             14801..15679
                     /gene="tsx"
                     /locus_tag="ACHOQY_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9676869.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNFN
                     VRSTGWGGYLVVGYNF"
     gene            complement(15856..16203)
                     /gene="yajD"
                     /locus_tag="ACHOQY_000720"
     CDS             complement(15856..16203)
                     /gene="yajD"
                     /locus_tag="ACHOQY_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9676870.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16332..17303)
                     /gene="secF"
                     /locus_tag="ACHOQY_000721"
     CDS             complement(16332..17303)
                     /gene="secF"
                     /locus_tag="ACHOQY_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9676871.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17314..19161)
                     /gene="secD"
                     /locus_tag="ACHOQY_000722"
     CDS             complement(17314..19161)
                     /gene="secD"
                     /locus_tag="ACHOQY_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9676872.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19189..19521)
                     /gene="yajC"
                     /locus_tag="ACHOQY_000723"
     CDS             complement(19189..19521)
                     /gene="yajC"
                     /locus_tag="ACHOQY_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9676873.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19544..20671)
                     /gene="tgt"
                     /locus_tag="ACHOQY_000724"
     CDS             complement(19544..20671)
                     /gene="tgt"
                     /locus_tag="ACHOQY_000724"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9676874.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20727..21797)
                     /gene="queA"
                     /locus_tag="ACHOQY_000725"
     CDS             complement(20727..21797)
                     /gene="queA"
                     /locus_tag="ACHOQY_000725"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9676875.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21890..22471
                     /gene="acpH"
                     /locus_tag="ACHOQY_000726"
     CDS             21890..22471
                     /gene="acpH"
                     /locus_tag="ACHOQY_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9676876.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22476..24293)
                     /gene="malZ"
                     /locus_tag="ACHOQY_000727"
     CDS             complement(22476..24293)
                     /gene="malZ"
                     /locus_tag="ACHOQY_000727"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9676877.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24449..25822)
                     /gene="proY"
                     /locus_tag="ACHOQY_000728"
     CDS             complement(24449..25822)
                     /gene="proY"
                     /locus_tag="ACHOQY_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9676878.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25898..27217)
                     /gene="brnQ"
                     /locus_tag="ACHOQY_000729"
     CDS             complement(25898..27217)
                     /gene="brnQ"
                     /locus_tag="ACHOQY_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9676879.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27624..28919)
                     /gene="phoR"
                     /locus_tag="ACHOQY_000730"
     CDS             complement(27624..28919)
                     /gene="phoR"
                     /locus_tag="ACHOQY_000730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9676880.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28977..29666)
                     /gene="phoB"
                     /locus_tag="ACHOQY_000731"
     CDS             complement(28977..29666)
                     /gene="phoB"
                     /locus_tag="ACHOQY_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9676881.1"
                     /translation="MTRRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29856..31058
                     /gene="sbcD"
                     /locus_tag="ACHOQY_000732"
     CDS             29856..31058
                     /gene="sbcD"
                     /locus_tag="ACHOQY_000732"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9676882.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31055..34198
                     /gene="sbcC"
                     /locus_tag="ACHOQY_000733"
     CDS             31055..34198
                     /gene="sbcC"
                     /locus_tag="ACHOQY_000733"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9676883.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34324..35508
                     /gene="araJ"
                     /locus_tag="ACHOQY_000734"
     CDS             34324..35508
                     /gene="araJ"
                     /locus_tag="ACHOQY_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9676884.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35651..36559)
                     /gene="mak"
                     /locus_tag="ACHOQY_000735"
     CDS             complement(35651..36559)
                     /gene="mak"
                     /locus_tag="ACHOQY_000735"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9676885.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36684..37595
                     /gene="rdgC"
                     /locus_tag="ACHOQY_000736"
     CDS             36684..37595
                     /gene="rdgC"
                     /locus_tag="ACHOQY_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9676886.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37673..37756)
                     /locus_tag="ACHOQY_000737"
     CDS             complement(37673..37756)
                     /locus_tag="ACHOQY_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676887.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37753..38094)
                     /locus_tag="ACHOQY_000738"
                     /pseudo
     CDS             complement(37753..38094)
                     /locus_tag="ACHOQY_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38242..38526)
                     /gene="ppnP"
                     /locus_tag="ACHOQY_000739"
     CDS             complement(38242..38526)
                     /gene="ppnP"
                     /locus_tag="ACHOQY_000739"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9676888.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38598..39275)
                     /gene="aroM"
                     /locus_tag="ACHOQY_000740"
     CDS             complement(38598..39275)
                     /gene="aroM"
                     /locus_tag="ACHOQY_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9676889.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39533..39724)
                     /gene="yaiA"
                     /locus_tag="ACHOQY_000741"
     CDS             complement(39533..39724)
                     /gene="yaiA"
                     /locus_tag="ACHOQY_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9676890.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39774..40298)
                     /gene="aroL"
                     /locus_tag="ACHOQY_000742"
     CDS             complement(39774..40298)
                     /gene="aroL"
                     /locus_tag="ACHOQY_000742"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9676891.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40481..40939)
                     /gene="yaiI"
                     /locus_tag="ACHOQY_000743"
     CDS             complement(40481..40939)
                     /gene="yaiI"
                     /locus_tag="ACHOQY_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9676892.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41059..41868
                     /gene="proC"
                     /locus_tag="ACHOQY_000744"
     CDS             41059..41868
                     /gene="proC"
                     /locus_tag="ACHOQY_000744"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9676893.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41885..43000)
                     /gene="adrA"
                     /locus_tag="ACHOQY_000745"
                     /gene_synonym="dgcC"
     CDS             complement(41885..43000)
                     /gene="adrA"
                     /locus_tag="ACHOQY_000745"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9676894.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43102..43422)
                     /gene="psiF"
                     /locus_tag="ACHOQY_000746"
     CDS             complement(43102..43422)
                     /gene="psiF"
                     /locus_tag="ACHOQY_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9676895.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43541..44956)
                     /gene="phoA"
                     /locus_tag="ACHOQY_000747"
     CDS             complement(43541..44956)
                     /gene="phoA"
                     /locus_tag="ACHOQY_000747"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9676896.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45057..45317)
                     /gene="iraP"
                     /locus_tag="ACHOQY_000748"
     CDS             complement(45057..45317)
                     /gene="iraP"
                     /locus_tag="ACHOQY_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9676897.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(45499..45702)
                     /locus_tag="ACHOQY_000749"
     CDS             complement(45499..45702)
                     /locus_tag="ACHOQY_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9676898.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            45780..46874
                     /gene="ddlA"
                     /locus_tag="ACHOQY_000750"
     CDS             45780..46874
                     /gene="ddlA"
                     /locus_tag="ACHOQY_000750"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9676899.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(46898..47110)
                     /gene="yaiZ"
                     /locus_tag="ACHOQY_000751"
     CDS             complement(46898..47110)
                     /gene="yaiZ"
                     /locus_tag="ACHOQY_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9676900.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            47370..47678
                     /gene="yaiY"
                     /locus_tag="ACHOQY_000752"
     CDS             47370..47678
                     /gene="yaiY"
                     /locus_tag="ACHOQY_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9676901.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(47737..48831)
                     /gene="yaiW"
                     /locus_tag="ACHOQY_000753"
     CDS             complement(47737..48831)
                     /gene="yaiW"
                     /locus_tag="ACHOQY_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9676902.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(48844..50064)
                     /gene="sbmA"
                     /locus_tag="ACHOQY_000754"
                     /pseudo
     CDS             complement(48844..50064)
                     /gene="sbmA"
                     /locus_tag="ACHOQY_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
     gene            50416..51573
                     /gene="ampH"
                     /locus_tag="ACHOQY_000755"
     CDS             50416..51573
                     /gene="ampH"
                     /locus_tag="ACHOQY_000755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9676903.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(51574..52197)
                     /gene="iprA"
                     /locus_tag="ACHOQY_000756"
     CDS             complement(51574..52197)
                     /gene="iprA"
                     /locus_tag="ACHOQY_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP9676904.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(52285..54982)
                     /locus_tag="ACHOQY_000757"
                     /pseudo
     CDS             complement(52285..54982)
                     /locus_tag="ACHOQY_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(55012..>55414)
                     /locus_tag="ACHOQY_000758"
     CDS             complement(55012..>55414)
                     /locus_tag="ACHOQY_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9676905.1"
                     /translation="SRAVIGWSMSPRMTAQLACDALQMALWWRKRPRNVIVHTDRGGQ
                     YCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMRATV
                     FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT        13804 a        14670 c        13776 g        13164 t
ORIGIN      
        1 caacatatag agcattcatt ttatgaatgc aaaaaaacat acctgacaca caaattactc
       61 tcctctgtaa tattttctca ttaacactct gtttacccaa catgcgctcc gcactacact
      121 atgtgggcat ttttttaagg ggtggtaatg aacgattata aaatgacgcc aggtgagagg
      181 cgcgcgacct ggggtttagg gaccgtattc tcgttgcgca tgctgggcat gtttatggtt
      241 ctgccggttc tgaccacgta cggcatggct ctgcaaggtg ccagcgaagc attaatcggt
      301 attgccattg gtatttatgg tctgactcag gccgtttttc agattccgtt tggcctgctt
      361 tccgaccgca ttggtcgcaa accattaatt gtcggtgggc tggcggtgtt tgccgccggt
      421 agcgttatcg ctgcgctttc cgactccatc tggggaatta ttctgggccg ggcgctacaa
      481 ggctctggag caattgccgc cgccgttatg gcgctgcttt ccgatctcac gcgcgaacaa
      541 aaccgcacca aagcgatggc gtttatcggc gtgagctttg gcattacctt tgccattgcg
      601 atggtgcttg gcccgatcat cactcacaaa cttgggctgc acgcgctgtt ctggatgatc
      661 gctattctgg caacaaccgg cattgcgttg accatttggg ttgtacccaa cagtagcact
      721 cacgtactta atcgtgagtc cggaatggtg aaaggcagtt tcagtaaagt gctggcggaa
      781 ccgcggctgc taaaactcaa ctttggcatt atgtgtctgc acatgcttct gatgtctacg
      841 tttgttgccc tgcccggaca gctagctgat gcggggttcc cggcggctga acactggaag
      901 gtctatctgg cgacgatgtt aatcgccttt ggctcggtcg tgcctttcat tatctacgct
      961 gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg tcgggttgat cgtggttgcg
     1021 gaaattgtgt tgtggaacgc acaaacgcag ttctggcaac tggtggtcgg cgtgcagctt
     1081 ttctttgtgg cgtttaattt gatggaagcc cttctgccct cacttatcag taaagagtcg
     1141 ccagcaggtt acaaaggtac ggcgatgggt gtctactcca ccagccagtt tcttggcgtg
     1201 gcgattggcg gttcgctggg cggctggatt gacggcatgt ttgacggtca gggggtattt
     1261 ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg ccagtaccat gaaagaaccg
     1321 ccgtatgtca gcagtttgcg cattgaaatc ccggcggaca ttgccgcaaa cgaagcgtta
     1381 aaagtgcgtt tgctggaaac tgaaggcgtc aaagaagtgt tgattgcaga agaagaacat
     1441 tcagcttatg tgaaaatcga cagcaaagtg acgaatcgct ttgagataga acaggcaatt
     1501 cgtcaggcat aaaaaacgcg gcgaattgcc tgctgcgcta cgcttatcag gcctacaaag
     1561 tttctgaatt acaaaccttt gtaggccgaa tcaggcgttc atgccgcatc tgacatgaac
     1621 aaaacgcaca tagtcgcgat taatcgcgga agtttttgaa ctggaacggc tgaccgagat
     1681 cgccaccacg taccatcgcc atgacagcct gcaaatcatc acgagatttg cccgttacgc
     1741 ggatttcatc gccctgaatt tgcgcctgca ctttcagttt gctgtctttg atcatcttga
     1801 cgattttctt ctgggtcgcg ctctcaatgc cctgtttcag tttcgcttcc acaaaccagg
     1861 ttttaccgct atgaacgata ttctccggta catccagcga actgccttca atgccgcgct
     1921 tcagcagctt ggcacgcaga atatccagca actgattgac ctggaaatcg gactcgctca
     1981 acactttgat ggttttgctg gcgtcgttca gctcaaatga ggcttcaacg ttacggaagt
     2041 caaaacggga ctccacttcg cggctcgcgt tatcgaccgc gttacgtgct tcctgaagat
     2101 caacttcaga gacaatatcg aaagatggca tcttttcctc tcccttcatt tttgatgcga
     2161 agcataatac ccgcaaagtt aaataaccga cagtcctgca ccaggacgta tcccttaatt
     2221 gtgcatagtc gcaacagcat gcacaattaa gggatagcct tattgaacac ccggagtggt
     2281 tgcgggtgag gaggaacaat gaaaattacc gtattgggat gcggtgcctt agggcaatta
     2341 tggcttacag cactttgcaa acagggtcat gaagttcagg gctggctgcg cgtaccgcaa
     2401 ccttattgta gcgtgaatct ggttgagaca gatggttcga tatttaacga atcgctgacc
     2461 gccaacgatc ccgattttct tgccaccagc gatctgctcc tggtgacgct gaaagcatgg
     2521 caggtttacg atgccgtcaa aagcctcgcg tccacactgc ctgtaaccac gccaatactg
     2581 ttaattcaca acggcatggg caccatcgaa gagttgcaaa acattcagca gccattactg
     2641 atgggcacca ccacccatgc cgcccgccgc gacggcaatg tcattattca tgtggcaaac
     2701 ggaatcacgc atattggtcc ggcacggcaa caggatggcg attacagtta tctggcggat
     2761 attttgcaaa ccgtgttgcc tgacgttgcc tggcataaca atattcgcgc cgagctgtgg
     2821 cgcaagctgg ccgtcaactg tgtgattaat ccactgaccg ccatctggaa ttgcccgaat
     2881 ggtgaattac gtcatcatcc gcaagaaatt atgcagatat gcgaagaagt cgcggcagtg
     2941 atcgagcgcg aagggcatca tacttcagca gaagatttgc gtgattacgt gatgcaggtg
     3001 attgatgcca cagcggaaaa tatctcgtcg atgttgcagg atatccgcgc gctgcgccac
     3061 accgaaatcg actatatcaa tggttttctc ttacgccgcg cccgcgcgca tgggattgcc
     3121 gtaccggaaa acacccgcct gtttgaaatg gtaaaaagaa aggagagtga atatgagcgc
     3181 atcggcactg gtttgcctcg cccctggtag tgaagagact gaagccgtca ccactatcga
     3241 tctgctggtt cgcggcggta tcaaagtcac cactgccagc gtcgccagcg atggcaacct
     3301 ggccattacc tgctcgcgcg gcgtgaagct gctggcggat gcgccgctgg tcgaggtggc
     3361 tgatggcgaa tatgacgtga tcgtactgcc tggtggcatt aaaggcgcag agtgttttcg
     3421 cgatagtacc ctgctggttg aaaccgttaa acagttccac cgttccgggc gtatcgtcgc
     3481 ggctatttgc gccgcgccag ccaccgtgct ggtgccgcac gatatcttcc cgattggtaa
     3541 tatgaccggc ttcccgacgc tgaaagacaa aattcccgct gaacaatggc aggacaagcg
     3601 cgtcgtctgg gatgcacggg taaaattgct gaccagccag gggccgggga cagctatcga
     3661 ctttggtctg aaaattatcg acctgctggt tgggcgcgag aaagcccatg aagtggcatc
     3721 acaactggtg atggcggcag ggatttataa ttattacgag tagtgtcgga tgcggtaagc
     3781 gtcgcatctg accagatgcg acgttacaac cacaaattac gggcgatata ccttcacatt
     3841 gttaaagccc tgctcgcgca gatagagcgc ctgcagacgg ctcatcaccc cgcgctcaca
     3901 ccacagtagc caggttctgt tctggtcgag atcgccaaac ttggtgctca gtttatagaa
     3961 cggcagagaa actacatcaa tcccttcgac tttcagtggc ttatcttcct gttcatcgat
     4021 agaacggata tcgaggatca cgtcgttcgg gccgaagcca ttgacggttt ccacttccac
     4081 cacttcctgc tcggtctgct gggcgatttc gcggatatca acgttattcg cttcttcaac
     4141 cactttatcg agaatgctga agtcgaattt ctcttcttcc gcttcaatct tcgacttaac
     4201 cgctttcacc gtcgggcttt tggagataac accgcaatat tccggcatcg tgcgagcaaa
     4261 gtcttcggtg ccaatctggc gagccaggtt gatgatgtgc tctttgtcgt aagaaatcag
     4321 cggacgcagg atcagcgtat cggagacgtt atcaatcagg cgcaggttgg tcagcgtctg
     4381 actggacacc tggccgagcg cttcgccggt gaccagcgcc tgtacgccgt aacgttcagc
     4441 cactttagac gcggcacgca ccatcatacg tttgaggata acgcccatct gaccgtcgtc
     4501 gattttctcg agaatttccc cgacgaccgg ttcgaaatta atagcgacaa aacgcacgcg
     4561 gtgggagctg ccaaaacggt tccacagata atgcgccacc tgacgcacgc caatttcatg
     4621 cgccgcgccg ccaaggttaa agaaacagta atgcacgcgg cagccgcgac gcatcaacat
     4681 ataactggaa acaccggagt cgaaaccacc ggaaatgagc gacagcacat cttcctgggt
     4741 gccgatcggg aaaccgccaa taccttcgta gcggccttta atcagcagga gacgatcgtc
     4801 ttccacttcc agatggacag tcacttccgg attggtcagc ttcacgcgcg cggactcaat
     4861 atgctgattt aaaccgccgc cgacgtaacg ttccacatca atcgagctaa aatcatgttt
     4921 gccacggcgc ttcacgcgta cgcagaaggt tttgccttcc agctgatcgc gatactgaac
     4981 caacgctttc tcaaaaatat cgtgcatgtc ggtaaacggc acgtcttcga cttcgagaat
     5041 atggtggata cccggaatac gggtcagggc gtcgcgaata gccagacgct ggttttcatc
     5101 ttttgcgcga acttcgatgt tatcccagtg gcggacgaca gcgagcgtct catcatagtg
     5161 ctttaaaacg ttacgaatgt tcccggtaag gatttttata aagcgcaagc gcacagattg
     5221 gcttttgatg gtgatttccg ggaacaattt aatgataaac ttcatggcgg caatggttcg
     5281 ttggcaagcc ttaagcgact tgtataggga aaaatacagc agcccacacc tgcggctgca
     5341 tccaggcgcg gaagtatacc actaacatcg ctttgctgcg cacatcacct taccattgcg
     5401 cgttatttgc tatttgccct gagtccgtta ccatgacggg gcgaaaaata ttgagagtca
     5461 gacattcatt atgccgaaga aaaatgaggc tcccgccagc tttgaaaagg cgctgagcga
     5521 gctggaacag attgtaaccc gtctggaaag tggcgacctg ccgctggaag aggcgctgaa
     5581 cgagttcgaa cgcggcgtgc agctggcacg tcaggggcag gccaaattac aacaagccga
     5641 acagcgcgta caaattctgc tgtctgacaa tgaagacgcc tctctaaccc cttttacacc
     5701 ggacaatgag taatggactt tccgcagcaa ctcgaagcct gcgttaagca ggccaaccag
     5761 gcgctgagcc gttttatcgc cccactgccc tttcagaaca ctcccgtggt cgaaaccatg
     5821 cagtatggcg cattattagg tggtaagcgt ctgcgaccct tcctggttta tgccaccggt
     5881 catatgtttg gcgttagcac aaacacgctg gacgcacccg ctgccgccgt agagtgtatc
     5941 cacgcttact cattaattca tgatgattta ccggcgatgg atgatgacga tctgcgtcgc
     6001 ggtttgccga cctgccatgt gaagtttggc gaagcaaacg cgattctcgc tggcgacgct
     6061 ttacaaacgc tggcgttctc gattctaagc gatgccaata tgccggaagt gtcggatcgc
     6121 gacagaattt cgatgatttc tgaactggcg agcgccagcg gtattgccgg aatgtgcggt
     6181 ggtcaggcat tagatttaga cgcggaaggc aaacacgtac ctctggacgc gcttgagcgt
     6241 attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg ttcgccttgg tgcattaagc
     6301 gccggagata aagggcgtcg tgctctgcca gtactcgaca agtacgcaga gagcatcggc
     6361 cttgccttcc aggttcagga tgacatcctg gatgtggtag gagatactgc aacgttggga
     6421 aaacgccagg gtgccgacca gcaacttggt aaaagtacct accctgcact tctgggtctt
     6481 gagcaagccc ggaagaaagc ccgggatctg atcgacgatg cccgtcagtc gctgaaacaa
     6541 ctggctgaac aatcactcga tacctcggca ctggaagcgc tagcggacta catcatccag
     6601 cgtaataaat aaacaataag tattaatagg cccctgatga gttttgatat tgccaaatac
     6661 ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta
     6721 ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg
     6781 cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac
     6841 accccgtttg accaattgat ttgggatgtg gggcatcagg cttatccgca taaaattttg
     6901 accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggcctgca cccgttcccg
     6961 tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccatcagt
     7021 gccggaattg gtattgcggt tgctgccgag aaagaaggca aaaatcgccg taccgtctgt
     7081 gtaatcggcg atggcgcgat taccgcaggc atggcgtttg aagcgatgaa tcacgcgggc
     7141 gatatccgtc ctgatatgct ggtggtcctc aacgacaacg aaatgtcgat ttccgaaaat
     7201 gtcggcgcgc tcaacaacca tctggcgcag ctgctttccg gtaagcttta ctcttcactg
     7261 cgcgaaggcg ggaaaaaagt tttctctggc gttccgccaa ttaaagagct gctcaaacgt
     7321 accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgaaga gctgggcttt
     7381 aactacatcg gcccggttga cggtcacgat gtgctggggc ttatcaccac gctgaagaac
     7441 atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat
     7501 gaaccggcag aaaaagaccc catcactttc cacgccgtgc ctaaatttga tccctccagc
     7561 ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg
     7621 ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa
     7681 ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gttacttcga cgtggcaatc
     7741 gccgagcaac acgcggtgac ctttgccgcc ggtctggcga ttggtgggta caaacccatt
     7801 gtcgcgattt actccacttt cctgcaacgc gcctatgatc aggtgctgca tgacgtggcg
     7861 attcaaaagc ttccggtcct gttcgccatc gaccgcgcgg gcattgttgg tgctgacggt
     7921 caaacccatc agggcgcttt tgacctctct tacctgcgct gcataccgga aatggtcatt
     7981 atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac
     8041 gacggcccgt ccgcggtgcg ctacccgcgc ggtaacgcgg ttggcatgga actgacgccg
     8101 ctggaaaaac tgccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggcgatc
     8161 cttaactttg gtacgctgat gccagacgcg gcgaaagtcg ctgaatcgct gaacgctacg
     8221 ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt taattctgga aatggccgcc
     8281 agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggcagcggc
     8341 gtgaacgaag tgctaatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgcct
     8401 gacttcttta ttccacaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc
     8461 gccggtatgg aagccaaaat caaggcctgg ctggcataat ccctactcca ctcctgctat
     8521 gcttaagaaa ttattcatag actctaaata attcgagttg caggaaggcg gcaaacgagt
     8581 gaagccccag gagcttacat aagtaagtga ctggggtgaa cgaatgcagc cgcagcacat
     8641 gcaacttgaa gtatgacgag tatagcggga gtggcagcat gcaatacaac cccttaggaa
     8701 aaaccgacct tcgcgtttcc cgactttgcc tcggctgtat gacctttggc gagccagatc
     8761 gcggtaatca cgcatggaca ctgccggaag agagcagccg tcccatcatt aaacgcgcgc
     8821 tggaaggcgg cataaatttc tttgataccg ccaatagcta ttccgatggc agcagcgaag
     8881 agatcgttgg tcgcgcactg cgggatttcg cccgtcgtga agacgtggtc gttgcgacga
     8941 aagtgttcca tcgcgttggt gatttaccgg aaggattatc ccgtgcacaa attttgcgct
     9001 ctatcgacga cagcctgcgc cgtctcggca tggattatgt cgatatcctg caaattcatc
     9061 gctgggatta caacacgccg atcgaagaga cgctggaagc cctgaacgac gtggtaaaag
     9121 ccgggaaagc gcgttatatc ggcgcgtcat ccatgcacgc ttcgcagttt gctcaggccc
     9181 tgaagctaca aaaacagcac ggctgggcgc agtttgtcag tatgcaggat cactacaatc
     9241 tgatttatcg cgaagaagag agcgagatgc tgccactgtg ttatcaggag ggcgtggcgg
     9301 tgattccgtg gagcccgctg gcgcgggggc gactgacgcg tccgtgggga gaaactaccg
     9361 cacgactggt gtctgatgag gtggggaaaa atctctataa agaaagcgat gaaaatgacg
     9421 cgcagatcgc agagcggtta acaggcgtca gcgaagaact cggtgcaaca cgagcacaag
     9481 ttgcgctggc ctggttgttg agtaaaccgg gcattgccgc accgattatc ggaacttcgc
     9541 gcgaagaaca gcttgatgag ctattgaacg cggtggatat cactttgaag ccggaacaga
     9601 ttgccgaact ggaaacgccg tataaaccgc atgcggtcgt aggatttaaa taagagtcca
     9661 ggcctgatga gacgtgacaa gcgtcacatc aggcatcggt gcacaactac gacagaatac
     9721 ccagcggcca gtgatgaccg ataaagtaca ggatgcctgc ggaaatcacc ccggcgacaa
     9781 tatcgtcgat catgatcccc atgccgccat gtacattgcg atcaaaccag cggatcggcc
     9841 acggcttcca catatccaga atacggaaaa tcacaaaccc ggcggcaacc cactgccagt
     9901 cattggtcgg cagcgccatg agcgtgatcc acataccaat aaattcgtcc cagacaatgc
     9961 tgccgtgatc gtgcacgccc atatctttcg ccgtttgatg acaaagatag acgccgatac
    10021 agatccccaa catcaccacc agcgagtaga gctgccaggg taaaaaggtc atcagatacc
    10081 agaacggaat cgctgccagc gatcccatcg tgccaggaac gattgggctt aatccacttc
    10141 cgaatccgac agcaagcaga tgccacggat tactcatctt caggcgactt ttcgcgacat
    10201 ctttatggcg tggcaaaatg gtcatatcct ttccagtcaa acgtgacagg ttcgccgtca
    10261 cgaataaaac aaagcccttc gatatcggcg gtcatttgcc cgatacaggt aaacggtacg
    10321 cccaggtgtc cgagagccac atccagcgcg ccacggttca gttccggcac agtgaaacac
    10381 aactcgtaat cttcaccgcc agagagcgcc cagcgcagcg cctgttccgg ttcaacatgg
    10441 cgagaaagcg catcagaaaa cggcagcaat gccaggtcaa tacgtgcgcc gcagtcgctg
    10501 gctttcacga tatgcccgag atcggaaatc agaccgtcag agagatcgat agctgaattt
    10561 gccagatcgc gcagtgcctg cccctgtaaa atacgcggcg atggacggag atgacgtttg
    10621 atcaagtagt cagcatcttt agcatcggca acctgcaaac ggttttgcaa aatcgccagc
    10681 ccggcggcgc tatcgcccgg tgtaccggtc acatagatcc agtcacccgg tttcgcccca
    10741 gagcgcgtta aggctcgtcc catcggaaca aagccgtgga tacccaacgt cattgataat
    10801 ggcccacgcg tggtatcgcc gccaatgagt tgcatatcgt aataattgag aagatcaaac
    10861 aaactgtcgc tgaaggactc aagccacgct tcgtctacgt ccggtaaggt taatgccagc
    10921 gtcagccagg ccggatcggc ccccatcgct gccagatcgc ttaggttcac tgccagtgct
    10981 ttatacgcca gatcagcagg atcgatatca tggaggaagt ggttgcccgc caccagcgta
    11041 tcagtgctga tcgccagggt ctgtttctcg gggatattga gaagagcgca atcgtcgccg
    11101 atgcccagtt cgacatcaag acgagaactt cttacacggt caaaataacg ggcaatcagg
    11161 gagaactcgc cacatgccat acgttatgcc tcagcaggta aaaaagaaaa ggccggcgat
    11221 cgcggaacgg tcttccgtga atctaccggc ctggatatca ctttttgtta gggcgaatca
    11281 caggtgctgc tttatcgagt acgccgttga cgaacttatg gctgtcttct gcgccgaacg
    11341 atttcgccag ttcgatcgct tcgttaatgg ccactttgta tggcacatcg ctacgtttag
    11401 acagctcgta cagcgcaatg cgcagtactg ctttttctac ctgtcccagt tcttccagca
    11461 ggcgggacag gtatggcttc atcagtccgt cgaggtatgc ggtgttagtc gccaccccgg
    11521 ccagcagctc acggaagtac aggacgtcaa cgtcttttac atcctgttca gccaggaact
    11581 ggtattcaac atcagcgatg tcgttctggg acaactgcca ggagtagagc gcctggacgg
    11641 cacactcacg agcgcggcga cgagcagcag gtttcacgga tttcccctta ctaatttcag
    11701 gccttgatgg ctttcaatac attaatcatt tcaagcgcgg tcagtgcagc ttctgcacct
    11761 ttgttgccag ctttggtgcc agcacgttcg atcgcttgtt caatgctttc agtggtcaga
    11821 accccaaaag caaccggaat ttcgctgtcc tgggcaacat gcgccaggcc gttgcttgca
    11881 ccaccagcga catattcaaa gtgggcagtg ccaccacgaa taaccgtacc cagcgcaatc
    11941 accgcgtcgt atttaccggt tttagccagt gcacccgccg ccagcggcag ctcataggca
    12001 ccaggcaccc aaacaacggt aatgttttca tcttttacct gaccgatacg tttcagtgcg
    12061 tcaattgcac cttccagcag gctgtcattg ataaagttgt tgaaacgcgc gatggtgatg
    12121 gcgacgcgag cgtccggggt agcaacgtta gcttcaataa tgttcatttt cttccttcgg
    12181 gttcgagtat ggccccgcag gggggcggat tttagcataa tatttcgtgc gctgcttccc
    12241 tttcgagccg ggagatcatg cacccactaa atgcaggcaa acatccgggc ctacatgacg
    12301 tatctctttg aatttaaatt ggggggcgtc ggctaatttc tcaagccctg gcagcgtgca
    12361 taatccgcgg gcgtcgctgc ctaatagttt aggtgcgata tagacaatca gctcatcgac
    12421 taaacccgcc tgcagcaatg cgccagcgag cgttggcccc gcttccaccc agatactgtt
    12481 aatttgctgt ttacccagtt gcatcatcag tacaaccaga tccagatgac ctttatgctc
    12541 tggaatcagc aaggtacgca ccgtttccgg ccactcacta gaatcttcct gcgtacgcgc
    12601 gaaccaggtt tcgccgggct gctgcacaat gcgatgttcc ggcgtcacgc gattttggct
    12661 atcaatcaca atacgtactg gctggcggag attttgttgc gggtagagcg cctgagtttg
    12721 ttcatccagt tcagaccaac gcactgttaa ggctggatca tccgccagca ccgtggcgct
    12781 gctagttaaa atggcatgac tttgtgcgcg ctgtcgttgt acgtcgcgcc gcgcctgagg
    12841 cgaagtgatc cactggcttt cgccgctcgc catcgccgtg cggccatcaa gcgatgcgcc
    12901 aagttttaac tgaatataag gaaagccggt gcgcatccgc ttgagaaagc ctttattcaa
    12961 ttgctcggct tcactcatca tcagcccgtg gctgacgtca atgccagcct gttgcagacg
    13021 gtaaagtcca cgcccagcga cctgcgggtt aggatcttgc atcgaggcaa ccacgcgcgc
    13081 tacgccagcg gcgatgagtg cgtcacagca cggtggcgta cgaccatgat ggctacaggg
    13141 ttcgagtgtg acataggcgg tcgcaccttt ggctttttca cccgccatac gcaacgcgtg
    13201 tacttcggca tgtggttcac ccgcacgttg gtggtaacct tcaccgacaa tttcgccatc
    13261 tttgacaatg acgcacccga cattcgggtt gggatgcgtg gtaaaacgtc ctcgttgcgc
    13321 cagctttagc gctcgcgcca tgtaatactc gtcctgcacg gcttagtcct ccaggcgcgc
    13381 gatctcttcg ccaaattctt tgatatcttc gaaactgcgg tagacagagg caaaacggat
    13441 ataggcgact ttatcgagct ttttcaattg ctccatcacc agattgccaa tcatcttgct
    13501 cggcacttcg cgctcaccgg tggcgcgcag ctgcgattta atatgattga ttgccatttc
    13561 gacgtcatcg gaactcaccg gacgtttttc cagcgcccgc agcattccgc tacgcaattt
    13621 ctcttcatta aacggttcac gcacgtcgtt gctttttaca acacgcggca taaccagctc
    13681 cgccacttca aaggtggtga aacgttcatt acacaccaga cactgccgac ggcggcgtac
    13741 ggatgaaccc tcgcccacga gacgagagtc aattacctta gtgtccacgg cgaaacagaa
    13801 tgggcaatgc atacggcgtc ctgaccaggt ggttaacaga aatctatttt accctgaact
    13861 gacgatacaa caaaggcgca acgcttttga gataaaggat cgatgctttc gctgctttta
    13921 ccctctacca ttaggctaat tgcgacgaga aagggacgac ctatgaatac taacgttttt
    13981 cgactgctcc tgctgggaag cctgttcagc cttagcgcct gtgtgcagca gagcgaagtg
    14041 cgacagatga aacacagcgt cagcacgctg aaccaagaga tgacgcaact caataaagaa
    14101 acggtcaaaa tcactcagca aaacaggctg aatgcaaaat ccagcagtgg ggtttacctt
    14161 ctgccaggag cgaaaacacc ggcaagactg gaaagccaga tcggtacttt acgtatgtcg
    14221 ctggtgaata ttacgcctga tgcagatggc accaccctga cgctacgtat tcagggcgag
    14281 tcgaatgatc ccttgcccgc attcagcgga actgttgaat atgggcaaat tcagggaaca
    14341 atagacaact ttcaggaaat caatgtgcag aatcaattga ttaatgcccc tgccagcgtc
    14401 ctcgccccca gcgatgttga tattccgtta cagttaaagg gtatttctgt agaacagtta
    14461 gacttcgtac gtatccacga tattcaacct gttatgcagt aaacgtattt cggggcgatt
    14521 ttgtgcgtct cgcaacatct ttccccgtca ttttgttact ccgcttacat caccctgatt
    14581 gatagtaaaa gtttgcaaca agggtgaaag tcagtacaat ccctgcccga atgtgtgtaa
    14641 acgtgaacgc aatcgattac gtaaatgata gaactgtgaa acgaaacata tttttgtgag
    14701 caatgatttt tataataggc tcctctgtat acgaaatatt tagaaacgca atttgcgcct
    14761 ttttcactcc cgcaagggat tttcaaacag tggcatacat atgaaaaaaa cattactggc
    14821 agccggtgcg gtactggcgc tctcttcgtc ttttactgtc aacgcagctg aaaacgacaa
    14881 accgcagtat ctttccgact ggtggcacca gagcgttaac gttgtcggaa gctatcacac
    14941 ccgtttcgga ccgcagatcc gcaacgatac ctaccttgag tacgaagcat tcgctaaaaa
    15001 agactggttc gacttctatg gttatgcgga tgcgccggta ttcttcggcg gtaactccga
    15061 tgctaaaggt atctggaacc acggttctcc gctgtttatg gaaatcgaac cacgtttctc
    15121 catcgacaag ctgaccaata ctgaccttag cttcggtccg ttcaaagagt ggtacttcgc
    15181 gaacaactac atttacgaca tgggtcgtaa taaagatggt cgccagagca cctggtacat
    15241 gggtctgggt accgacatcg atactggtct gccgatgagc ctgtccatga acgtctatgc
    15301 gaaataccag tggcagaact atggcgcagc gaacgaaaac gagtgggacg gttaccgttt
    15361 caaaattaaa tactttgtgc cgattaccga tctgtggggc ggtcagctga gctacatcgg
    15421 cttcaccaac ttcgactggg gttccgattt aggggatgac agcggtaacg caatcaacgg
    15481 tattaagacc cgtactaata actctatcgc ttccagccat attctggctc tgaactacga
    15541 tcactggcac tactctgtcg tagctcgtta ctggcacgac ggtggtcagt ggaacgacga
    15601 tgcagaactg aacttcggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    15661 tgtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttcagattgt
    15721 taacaacgtg cgctttgttc atgccggatg cggcgtaaac gctttgtccg acccacaaaa
    15781 gcacgtaaat tcaatatatt gctgaaataa cgtagtcctg ataagcgtag cgcatcaggc
    15841 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt
    15901 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    15961 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac aaagcaattc
    16021 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacagtaag
    16081 ttcacgcagg ttggaataaa caaactcgcg ggaacagcga ccgcagaccc acggatatat
    16141 ttttaatgct ttttcgcgat agccgctttc taaccgcgca tagttttttg ggatgatagc
    16201 catgaagagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    16261 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    16321 cgggaacttg attacggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    16381 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc cacatagatg
    16441 gaagatgcag taccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    16501 ggaccaccga agaggtacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    16561 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    16621 atcttgcgga agttttcacg aatacggtcc gataccacga tactgtcgtt aagcgagtaa
    16681 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    16741 aaaataccca gcgtaatgat aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt
    16801 cgccactcaa agcgaaaacc tacgtacaca aggatagaca gcagcgccgc catcaacgcc
    16861 atcgcaccgg tttgcgcaag gtctgccccc acgctcggac cgacgaactc aatacgcttc
    16921 actgctgcat tctgattggt ggattcgtta atcaccttca gaacctggct gcccagcacc
    16981 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    17041 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    17101 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtgaa atccagcccc
    17161 cagttaaagc cgcgcacgcc cataataacg atagcggcga ttaacagcag accagagatg
    17221 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    17281 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    17341 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    17401 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    17461 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    17521 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    17581 acgttcgttg atcagtacgt tcgcatcgac cgccaccgca agggttaaga cgatacccgc
    17641 gatacctggc atactcagcg ttgcgcctgg caacagcgac ataatgccga cgattaagat
    17701 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    17761 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgtt caatgttctg
    17821 catacccagg gttgggccaa tggtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    17881 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    17941 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    18001 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    18061 ggttgccatc ggtttgccga tgttgtcctt agtgaagtta gacatgatgt taccaccagc
    18121 gctatcgagc gagatgttaa cctgcggttg gttgtattcg tcctggctgg aagtggagtc
    18181 ggtgatatgg tcaccggtca gaattacgcg tttgtacagc acaactggct gaccttcgcg
    18241 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctggtcaac
    18301 gttggtgttt accagacgga attccagcgt tgccgtcgca cccagaatct ctttcgcacg
    18361 cgcagtgtcc tgaatacctg gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    18421 caccggctcc gccacgccaa gttggtttac acggttacgc aggatattga tgttctgctg
    18481 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    18541 gctgccctgg ctactaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    18601 atcacgagct ttagcatcgc ggaaagtaat gctcaggccg tagttgtttt ctttacgaac
    18661 ggtggtatac gggatgccct tttcacgcag atcgctgcgc aggctatcga tattttgttc
    18721 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    18781 caggtcaagg ccgagcttca tcggctcagc gtgaatagct gccagccagc gcggcgttgc
    18841 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    18901 agcgcgcagc tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    18961 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    19021 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    19081 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    19141 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    19201 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    19261 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc gaccaaccag
    19321 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    19381 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    19441 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    19501 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    19561 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    19621 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    19681 tgttgagtcg cgcgcctaat atttcgttgc aacggtcaag atgatgcaag taagcgcgtg
    19741 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    19801 acttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    19861 tgcgggttgg cattacgcag tcaaacatat cgataccacg acgtacgcct tcaaccaggt
    19921 cttctggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcgggcata
    19981 catgctccag aatgcggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    20041 cgtagccatc aaaaccgata tctaccagac ctttaacaga aatatcacgt aaatcttcgt
    20101 aaacgctgcc ctgaatgata ccaaacagcg catttttgtt tccgagactg tcaaaacgct
    20161 cacggctacg cttcgcccaa cgcagagaca tctccatgga gcgttttgcg taatcccagt
    20221 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    20281 gaatctccat tgatttttca ggatcgagga aaatcggatc gccgttgatc gggttacgga
    20341 agtgcacgcc ctgttcggtg attttacgaa tatcgccaag gctgaagacc tggaagccgc
    20401 cggagtcggt gaggatcggc cccttccact gcataaaatc gtgcagatcg ccgtgcagtt
    20461 tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg
    20521 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    20581 gcataaaaca aggcgtttcc actacgccac aatcaaagac caggcggcca cggcgtgcgc
    20641 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    20701 caacgtttta aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga
    20761 ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgct
    20821 gctttatagg cgttcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc
    20881 gtcgattccg gcaggtggaa gtttgtcacc agcgcatcga ccactttgta ctggaagccc
    20941 ggatagataa agatttgggt atcgtcaaag aacggttcga taagatcgtt tttcgccgcc
    21001 tgagccgcgc tttccagcga acgtactgag gtggtgccaa ccgcaatcac ccggttaccg
    21061 cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gcacttcagc gtattccgag
    21121 tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg
    21181 tgcaacgtca caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggttcg
    21241 tcaaaatgca gacctgcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt
    21301 tgataaagtt cgcggtcagc gtcttcgtcc ggacggtcga tatacggcgg cagcggcata
    21361 tggccgatgc tgttgagaat atccagcacc gagcgttcat cattaaattc gacttcaaac
    21421 agtgcgccgt ggcgcgcggt cattgttgcg ttaatacttt cgtcatcgcc cagcagcagt
    21481 tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg
    21541 agcatccgtt caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacaggcgc
    21601 gccgggatca cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcaagt
    21661 aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga
    21721 cagctactgc gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag
    21781 aaatcggtaa cgcgcatgac actgactcag actaaaataa gaggcgggta gtctagtgcc
    21841 ggggcgctct ccctgcaacc tttaccccct tttggataga atataatcga tgaatttttt
    21901 agctcacctg catttagccc atctcgcgga aagctcgctt tccggcaatt tactggctga
    21961 tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat
    22021 gcatcgacgt atcgacgtat taaccgacaa tctgccggaa gtccgcgaag cacgggaatg
    22081 gtttcgtagt gaaactcgcc gcgttgcgcc tattacgctg gatgtcatgt gggatcactt
    22141 tctttcccgc cactggtcgc agctgtcgcc ggactttccg ctacaggaat ttgtctgtta
    22201 tgcccgcgag caagtgatga cgattttgcc ggactcaccg ccacgtttta tcaatctgaa
    22261 caattacttg tggtcagagc agtggctggt gcgctatcgc gatatggatt tcatccagaa
    22321 cgtgttaaac ggcatggcaa gccgccgccc acgtctggat gccctgcgtg actcctggta
    22381 cgatttagac gctcattatg acgccctcga aacccgcttc tggcagtttt atccgcggat
    22441 gatggcgcag gcgtcacaca aggcgttata aaacgttagt tcatccatac cgtagccgaa
    22501 atggcaggca aagccagaat cccgtcagtc agttgcccat gcccttcttt gcattgccat
    22561 tgcacggcat tgagcaacgg tgacgcgggt agcaccactt cacaagcttc gccacggttg
    22621 attgccacca gtacacgctg ctgattcagc acgcggacaa ataccaccac gttatcttcc
    22681 gcatacagca cctgacagcc gccatgacgt agcgcctgac ttttcttacg cagcgcaatc
    22741 attcgctggt acagcgcgaa taacgccgta tcctgctttt ccacctgcca ggggaacggt
    22801 ttacggcaaa acggatcgtt tttgccatcc agtcctactt catcaccgta ataaatgcac
    22861 ggtacaccag gccaggtgaa cagccagacc accgccagcg gcaggcgcgc aatatcccga
    22921 ccgagcagcg ttttaaatcg cgcagtatcg tggctgtcga gctgattaaa catacgtaat
    22981 tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc aggccataca ggtttgggca
    23041 tcaatttgct gcggatcgta agagatatcg gtattggcaa gaaatcccca caacgggaat
    23101 gtgaagccac gatagttcat ggcggcatct tccacatcgg cctgtaacca ttgccgtgca
    23161 tcgccaaaat gttcgccgac aatatacgct tccggctggg tttctttcgc cgcttcggtg
    23221 atcccagcaa cgtgctgcat attattgcgc gccccacccg cctcccccag catatgcacc
    23281 acatccagcc gccagccgtc catactccac ggcgctttca gccaatggcg gacaatactg
    23341 tcttccccgc gataaatttc attcaccaga ctttccgact gataatccag cttcggcaag
    23401 ctggcatagc caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc
    23461 cagggcgatt cagggttgtg acaagctccc cccgtgccac gattgtgcct gtcaaaccag
    23521 gcatgggaat cgccactgtg gttaaacacg ccatccagca ccagccgcat tcccagctgc
    23581 tgcgtattgt ggcgtaagcg cagcaacgcc ccatcaccgt caaactgcgg atcgacatgg
    23641 cgataatcct cggtatcgta tttatgtacg ctgggagctt taaacaccgg attgagatac
    23701 agcgccgtca cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg
    23761 ccgccataga acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag
    23821 atgatctctt gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga
    23881 agactacgcg caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt
    23941 gggccgatat ccggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg
    24001 aagccctgcg gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa actgtaacgc
    24061 cgccggggtt gtccgctgga gagatcaatc gccgcacgcc atgcggtgac gccaggctgc
    24121 tgctgactgc gctgcttatg catcgatact gacatttctt cgttatcgtg ttctgtacgc
    24181 agcataatgc gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag cagttgatct
    24241 ttgctttgtt taacaaatgg gggcaccggc aggtgccatg catttaacat cataattccc
    24301 ctgtgatgaa aatctgatca ccttgccata gcctaatgca ggatcactca tcatctgaag
    24361 gatatttggg ggaggaggac gggggcggat aactttcgga atagccggat caggatttcc
    24421 tcctgaaccg gcttacagaa cgcaagtatt attggttttc agccagctga cgatcgtggc
    24481 ggcgtttaaa catccagcca atcaacagca caacaatcca cgcgaaaccg acatacagcg
    24541 agatacgcgt atccgggtga taaccaatca acccgataat aaagagcagg aaaatcagcc
    24601 cgccgatggt cgttgctacc ccacccggca ctttaaattt cagcgcctta acttcttctg
    24661 gcggcaaacg gcgacggaag gcaatttgcg acagcaggat cataatccac acccacaccg
    24721 tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt ttccggcatg atgtagttca
    24781 gatacaccgc aaacagcagc gcggtagtca tcaccagcac cgtaacccac ggaataccgc
    24841 gacgcgacgt tttgctgaaa attttcggcg cgctgccctg ctctgccata ccgtggagca
    24901 tacggcctac gccaaatacg tcactgttaa ttgccgacag cgaagcagtc agcacaacaa
    24961 agttaagaat gctggcggca aaggtaatgc ccatatgctg gaacgtcagc acgaacgggc
    25021 taccggcagt gccaacctga ttccacgggt agatagacat aatgacgaac agcgtaccga
    25081 cgtagaacac cagaatacgc atcggcacgg agttaatcgc acgcggtatc gatttctcag
    25141 gatctttcgc ttcaccggcg gtaatcccga taatttcgat cccaccgtaa gcaaacatca
    25201 ccatttgcaa cgacatcacc atcccaagcc agccgttact gaagaagccg ccgttgctcc
    25261 acaggttatg aataccggtc ggttgcccgc cgttgccaat cccccagatg atgatgccga
    25321 aaccggcgac aatcatgatg atgatggtgg cgactttaaa gaacgagaac cagaattcca
    25381 gctcaccgaa gaccttcacg ctcatcaggt ttacggcgca aatgatcagc accacgctca
    25441 gtacccaaat ccagtgcggc accgtcggga accagacacc catatagata ccaaaagcgg
    25501 tcacatcggc gatggcgaca ataaggattt caaagcaata ggtccagccg gtaatgtaac
    25561 ctgccagcgg gccgaggttt tcctgcgcat aacgcgagaa agagctggct gccgggttat
    25621 gtaccgacat ttcccccagc gcacgcataa tgatatacgc cgcgatacca ccgataatat
    25681 aggccaacaa cacgctcgga ccggccattt tgatggcgtc tgccgaaccg taaaacagcc
    25741 cggtgccaat tgctgaaccc agtgccataa agcgtatgtg gcgggtactt agcccacgct
    25801 ttagcttgtt cttactttcc atcaattcct aacccatcaa cacaataaaa aaccacgggg
    25861 catcgcagcc ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg gaggtcacct
    25921 gacgacctgc cgcgcgatcc cagataatgg ccagaaccac catcaccact gttggcatta
    25981 accacgccag accttgttcg gccagcggta aacgctgcgc ccaggacggt aagatatcgc
    26041 tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag caggctgata aacatcggcg
    26101 gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat acaaccagtg
    26161 cgatacacgg cggataaata gcggtcagca ccggtacgga gatctggatc agctggctta
    26221 agccgaggtt agaaaccacc atcgagaagc cgccgaggat aaacaccagc gtacgataag
    26281 agagcggtac gtactgggca aagaattctg cacaagcaca ggtcaggcca actgccgtta
    26341 ccaggcaggc gatgaagatt aacgccgcca gcaggaagct accgccgccg ccaaaggtgt
    26401 gctgaacgta agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc
    26461 tgtctgaacc cagacggaac agcgccaggt acagcagagt cagaccaaca cccgccatca
    26521 ggccagccca gacggtataa cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg
    26581 ccgcgttaac aataacgata ccaaacacca ttgcgcccag cgtatccatg gtcagatagc
    26641 cgttaacgaa gccgttagaa aacgcagcgt tttgataagc ctcagtcgcc gtgctgatag
    26701 aacccgccgg ccagacaatc gcggcaacag acaggatgac cagcgcgata attttcagcg
    26761 gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg atagagcgaa accagaataa
    26821 cgatagcgaa atagaccagg ctgtagataa acagcggcag cgcggaatca cccgtcagcg
    26881 gcgcaatccc cacttcaaag gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca
    26941 ccgccaggta acaaaccgtt gccagcagta cgccagcgac tttaccgatt ggcgtgctga
    27001 ggctgtcaac accgccgcca acttttgcca gcgccactac cgttaatacc ggcaggccaa
    27061 cggcagtaat gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc tgtaagccga
    27121 ccattggagg gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca
    27181 gagcgatgat atcgcgcgat cttaattgat gggtcataaa tcatactgcc tgtggatgtg
    27241 gtgttgtaac gttaaatttt tgtgccaatc cctggggatg atacagcggt atatttaaac
    27301 aattttagcg ggaaatactc ccaatcaggc gcggcgaaga atagtttttc gatttaccgc
    27361 gttaatgcag tcagtatgac agcacctgaa gcgcaattta aacgcttata acgttttaaa
    27421 gcaaggaggg atctaaaaac cagaggtata tagcgataag aaatcaccaa ccagtataaa
    27481 acactatttc cgtgaaaaag acatggctaa tcatgcgaac aaaaaatcac caaccgttgt
    27541 aaaatctaac gcccgtattt tgactgcaaa gaaaataagc cagcatcggc tggcttatgg
    27601 caataaaaga tgacaaaggc ggattaatcg ctgtttttgg caattaaacg ttccgggata
    27661 acaaaactga aacgtgttcc ttttcctact gtactctcaa tattcaggcg actttcgtga
    27721 tgattcaccg catgtttcac gatcgctaac cctaatccgc taccgccggt ttgccgggaa
    27781 cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg
    27841 cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg gcactcgcag ccagcgtacg
    27901 gtgatatgcg tgccttccgg cgtatgattc acggcgttat agaccaggtt cgaaatcgca
    27961 ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt tatctatctc aaaggtaaat
    28021 gtctgttttt tctgactcag agtctgagcc tcgcgctcaa caacgcgcag catcatcggc
    28081 acatcaacct tttcattgag caattgcgtc ggcgcggctt caattttcga cagcgttagc
    28141 aactgcttca ccagcccttc catccgctgg gtctgctcgc gcatggtgtg caacgctttt
    28201 tcgcgtaccg cgccttccag cggctgctca tccatcatct ccaggtaacc ctgtaacacg
    28261 gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa agttacgccg cgccccttcc
    28321 agttgatgca tttgcgtgac atcacgcgcc accatcagca actgtttgtg ggtataaggc
    28381 atgacgcgaa tttccagatg ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa
    28441 tcacgcgttt tcagatattg cgtaaactcc gggtaacgca gcaggttaag gatgttctgc
    28501 ccgttatctt ccggccagcg caaaccaaga atttgttgcg ccagaccgtt acaccagaaa
    28561 ataccgccct cttccgtggt cagcaccact gcgtcgggca gcgactccgc gccgctacga
    28621 aagcgtttaa tcagattgcc cagttcacgg cggcgttttt tatttcgcag ctgcatctgg
    28681 tgtaagccgt atagtagcgg ttcccagcta ccacgccccg gtggcggggt catactacga
    28741 tccacccaca gccaccatga aaggcgcaat aaattccaga aatgccagat aagcagtccc
    28801 gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag gatgaaagcc
    28861 gggaggcagc aaagtagcag ctccagcacc agccttttcc acgacagccg ttccagcacg
    28921 taagatactc cagttaagaa atcataagcc ctgctctgcg tccgatgagc aaggcgttaa
    28981 aagcgggttg aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg
    29041 ccgggctcca gtgctttacg caggcgacga atgtggacat cgaccgtgcg gtcttccaca
    29101 tacacgttag ttccccagac gtggtttaac agctgctcgc ggctgtacac gcgctcagga
    29161 tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc ccatctccag cggctcttcg
    29221 cccgccatca ctcggtgaga tgtcgggtcg agacttaatc cctgcatctc aatcacctct
    29281 tccaccgcca ttggcgaaat acggcgcatt accgctttga ttcgcgccac cagctccttc
    29341 ggcgaaaacg gcttggtgat atagtcatcc gcgccggttt caaggccgcg cacgcgatct
    29401 tcttcttccc ctctggcggt caacatcacc actggaatat cccgggtcat cgactcgcgc
    29461 ttgaggtgtt tgatgaactg gataccggag ccgccaggta acatccagtc aaggagaatt
    29521 aaatccggcc agggttcatt cagttgattc acagcactgt cataatcttc cgcttcgacc
    29581 ggctgaaagc cattttgttc gagcacgaag cagaccattt cgcgaattgg agcttcatct
    29641 tctacgacca gaatacgtct cgtcatgatt tgccctgttg taataaatag gttgcgatca
    29701 ttaatgcgac gtcattatgc gtcagattta tgacagattt atgaaaagct cgtcgcacat
    29761 atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta
    29821 taatcgcgcc catgcttttt cgccagggaa ccgttatgcg catccttcac acctcagact
    29881 ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg
    29941 actggctgct ggagacagca caaacccatc aggtggatgc gattattgtt gccggtgatg
    30001 ttttcgatac cggctcgccg cccagttacg cccgcacgtt atacaaccgt tttgttgtca
    30061 atttacagca aactggctgt catctggtgg tactggcagg aaaccatgac tcggtcgcca
    30121 cgctgaatga atcgcgcgat atcatggcgt tcctcaatac taccgtggtc gccagcgccg
    30181 gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca
    30241 ttccgttttt acgtccacgt gacattatta ccagccaggc ggggcttaac ggtattgaaa
    30301 aacagcagca tttactggca gcgattaccg attattacca acaacactat gccgatgcct
    30361 gcaaactgcg cggcgatcag cctctgccca tcatcgccac gggacattta acgaccgtgg
    30421 gggccagtaa aagtgacgcc gtgcgtgaca tttatattgg cacgctggac gcgtttccgg
    30481 cacaaaactt tccaccagcc gactacatcg cgctcgggca tattcaccgc gcacagatta
    30541 ttggcggcat ggaacatgtt cgctattgcg gctcccccat tccactgagt tttgatgaat
    30601 gcggtaagag taaatatgtc catctggtga cattttcaaa cggcaaatta gagagcgtgg
    30661 aaaacctgaa cgtaccggta acgcaaccca tggcagtgct gaaaggcgat ctggcgtcga
    30721 ttaccgcaca gctggaacag tggcgcgatg tatcgcagga gccacctgtc tggctggata
    30781 tcgaaatcac tactgatgag tatctgcatg atattcagcg caaaatccag gcattaaccg
    30841 aatcattgcc tgtcgaagta ttgctggtac gtcggagtcg tgaacagcgc gagcgtgtgt
    30901 tagccagcca acagcgtgaa accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc
    30961 gtctggcact ggaagaactg gatgaatcgc agcagcaacg tctgcagcat cttttcacca
    31021 cgacgttgca taccctcgcc ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa
    31081 aaacctgaac tcattaaaag gcgaatggaa gattgatttc acccgcgagc cgttcgccag
    31141 caacgggctg tttgctatta ccggcccaac aggtgcgggg aaaaccaccc tgctggacgc
    31201 catttgtctg gcgctgtatc atgaaactcc gcgtctctct aacgtttcac aatcgcaaaa
    31261 tgatctcatg acccgcgata ccgccgaatg tctggcggag gtggagtttg aagtgaaagg
    31321 tgaagcgtac cgtgcattct ggagccagaa tcgggcgcgt aaccaacccg acggtaattt
    31381 gcaggtgcca cgcgtagagc tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt
    31441 gaaagataag ctggaactga cagcgacgtt aaccgggctg gattacgggc gcttcacccg
    31501 ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg
    31561 cgcggaattg ctcgaggagt taaccggcac tgaaatctac gggcaaatct cggcgatggt
    31621 ttttgagcag cacaaatcgg cccgcacaga gctggagaag ctgcaagcgc aggccagcgg
    31681 cgtcgcgttg ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc aggtacttac
    31741 tgacgaagaa aaacagttac ttaccgcgca gcagcaagaa caacaatcgc taaactggtt
    31801 aacgcgtcag gacgaattgc agcaagaagc cagccgccgt cagcaggcct tgcaacaggt
    31861 gttagccgaa gaagaaaaag cgcaacctca actggcggcg cttagtctgg cacaaccggc
    31921 acgaaatctt cgtccacact gggaacgcat cgcagaacac agcgcggcgc tggcgcatat
    31981 tcgccagcgg attgaagaag taaatactcg cttacagagc acaatggcgc ttcgcgcgag
    32041 cattcgccac cacgcggcga agcagtcagc agaattacag cagcagcaac aaagcctgaa
    32101 tacctggtta caggaacacg accgcttccg tcagtggaac aacgaactgg cgggttggcg
    32161 tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc agcaacagtt
    32221 aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc acgttgacgt taaccgccga
    32281 tgaagttgct accgccctgg cgcaacatgc tgagcaacgc ccactgcgtc agcgcctggt
    32341 cgcgctgcat gggcagattg ttccccaaca aaaacgtctg gcgcagttaa tggtcactat
    32401 ccagaatgtc actctggaac agacgcaacg taatgtcgca cttaacgaaa tgcgccagcg
    32461 ttataaagaa aagacgcagc aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg
    32521 catcaaaacg ctggaagccc agcgtgcaca gttacaggcg ggtcagcctt gtccactttg
    32581 tggttccacc agccatccgg cggtcgaggc gtatcaggcg ctggagcctg gcgttaatca
    32641 gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct
    32701 gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc gatgaaaacg aagcgcaaag
    32761 cctccgacaa gatgagcaag cacttactca acaatggcaa gccgtcacgg ccagcctcaa
    32821 tatcaccttg cagccacagg acgatattca accgtggctt gatgcacaag atgagcacga
    32881 acgccagctg cggttactca gccaacgaca tgaattacaa gggcagattg ccgcgcataa
    32941 tcagcaaatt atccagtatc aacagcaaat tgaacaacgc cagcagcagc tcttaacggc
    33001 attggcgggt tatgcactga cattgccaca ggaagatgaa gaagagagct ggttggcgac
    33061 acgtcagcaa gaagcgcaga gctggcagca acgccagaac gaattaaccg cgctgcaaaa
    33121 ccgtattcag cagctgacgc cgattctgga aacgttgccg caaagtgatg atctcccgca
    33181 cagcgaagaa actgtggcgc tggataactg gcggcaggtt catgaacaat gtctcgcatt
    33241 acacagccag cagcagacat tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa
    33301 agcccaggcg cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc agcaggcgtt
    33361 ccttgcggcg ctaatggatg aacaaacact aacgcagctg gaacagctca agcagaatct
    33421 ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa cgctggcaca
    33481 gcatcaacaa caccgacctg acgggttggc tctcactgtg acggtggagc agattcagca
    33541 agagttagcg caaactcacc aaaagttgcg tgaaaacacg acgagtcaag gcgagattcg
    33601 ccagcagctg aagcaggatg cagataaccg tcagcaacaa caaaccttaa tgcagcaaat
    33661 tgctcaaatg acgcagcagg ttgaggactg gggatatctg aattcgctaa taggttccaa
    33721 agagggcgat aaattccgca agtttgccca ggggctgacg ctggataatt tagtccatct
    33781 cgctaatcag caacttaccc ggctgcacgg gcgctatctg ttacagcgca aagccagcga
    33841 gacgctggaa gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg atacccgtac
    33901 cctttccggc ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc tttcggatct
    33961 ggtcagccat aaaacacgta ttgactcgct gttccttgat gaaggttttg gcacgctgga
    34021 tagcgaaacg ctggataccg cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac
    34081 catcggtgtg attagccacg tagaagcgat gaaagagcgt attccggtgc agatcaaagt
    34141 gaaaaagatc aacggcctag gctacagcaa actggaaagt acgtttgcag tgaaataact
    34201 attcagcagg ataatgaata cagaggggcg aattatctct tggccttgct ggtcgttatc
    34261 ctgcaagcta tcactttatt ggctacggtg attggtagcc gttctggtag ttgtgatggt
    34321 ggtatgaaaa aagtcatttt atctttggct ctgggcacgt ttggtttggg gatggccgaa
    34381 tttggcatta tgggcgtgct cacggagctg gcgcataacg taggaatttc gattcctgct
    34441 gccgggcata tgatctcgta ttatgcgctg ggagtggtgg tcggtgcgcc aatcatcgca
    34501 ctcttttcca gccgctactc actcaaacat atcttgttgt ttctggtggc gttgtgcgtc
    34561 attggcaacg ccatgttcac gctctcttcg tcttacctga tgcttgccat tggtcgcctg
    34621 gtttccggct ttccgcatgg cgcatttttt ggcgtcggcg cgatcgtgtt atcaaaaatt
    34681 atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg tttccgggat gacagtcgcc
    34741 aatttgctgg gcattccgct gggaacgtat ttaagtcagg aatttagctg gcgttacacc
    34801 tttttattga tcgctgtttt taatattgcg gtgatggcat cggtctattt ttgggtgccg
    34861 gatattcgcg acgaggcgaa aggaaagctg cgcgaacaat ttcacttttt acgcagcccg
    34921 gccccgtggt taattttcgc cgccacgatg tttggcaacg caggtgtgtt tgcctggttc
    34981 agctacgtaa agccatacat gatgtttatt tccggttttt cggaaacggc gatgaccttt
    35041 attatgatgt tagttgggct agggatggtg ttgggaaata tgctaagtgg caggatttca
    35101 ggacgttatt caccactgcg cattgcagca gtgactgact ttataattgt actggcactg
    35161 ctgatgctct ttttctgctg cggcatgaaa acaacgtcgc ttatttttgc ttttatttgt
    35221 tgcgcgggat tatttgccct ttcagcacct ctgcaaatat tgttactaca aaacgccaaa
    35281 ggcggagagt tattaggtgc cgcaggtggg caaatagcgt ttaacctcgg tagcgccgtc
    35341 ggcgcatatt gcggaggtat gatgctgacg ctggggctgg catataatta cgtggcgctg
    35401 cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc tgctgtatgg tcgctataag
    35461 cgccagcaag cggcggatag tccggtgctg gcgaaaccac tggggtagat tactgtcacg
    35521 gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta tccgccctac gcggttctgg
    35581 cacattttgt aggcctgata agacgcggca agcgtcgcat caggcatgat gcgccaattg
    35641 cctacgtttt ttactcttgt ggccataacc acgcagcgcc gcgtacgccg ctggaatcac
    35701 cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc gaagacaaat tgtttaatca
    35761 actgcggaac cgtttgatat aaacggtcta cattgctcat cccgcccccc aggacaatca
    35821 catccggatc gagaatattc acgacatgtg ccagcgattt tgccagccgc agctcgtagc
    35881 gacgcaatgc cagttccgct accggatcgc tttcttcaac caggctgata atttcactgc
    35941 ctttcagcgc atgtccgctc aaacgacgat aatccgtcgc gaatccagtg cccgaaataa
    36001 aggtttcaat acaaccttgt ttaccgcaat aacaagggac ttcctcgcga taacgcagtt
    36061 cgtcttcgtc catccacggt agcggattgt gtccccactc acctgccgtg ccattgccgc
    36121 cgatatgcgc ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg ataatcacgg
    36181 caaataccgt ctgcgctccc gctgccgcgc catctaccgc ttctgaaacc gccagacagt
    36241 tggcgtcatt tgccagccgc acttcccgct gcaaccgcgc gcttaagtct ttatcgaatg
    36301 gctgaccgtt gagccaggtt gaattggcat tcttcaccac gccggtgtag ggcgaaatcg
    36361 aacctggaat gcccatacct accgttccgc gctgccctgt cgcctgctcc gccatatcaa
    36421 ccaacgtggc gatcgtttca atagtttgcc ggtaatcatc acgcggcgtg ggcagacgat
    36481 ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac ttcagttttg gtgccgccta
    36541 aatcgatacc tatacgcacg gtactctcct tatttttttc aatatcaata gcgtagagac
    36601 ggacaaccgg attggcaatg caaggccgcc gacaattcgt tatcatgccc gctaaattta
    36661 acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt taccgtctta
    36721 gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg atggcattta
    36781 ccccatgcgg cagccaggac atggcgaaga tgggctgggt tcctccgatg ggatcgcaca
    36841 gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat ctgcgcgcgc aaagaagaaa
    36901 aaatcctccc gtctccggtg attaaacagg cgctggaagc gaaaatcgcc aaactggaag
    36961 cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac gaagtgctgc
    37021 actctctgct gccgcgtgct ttcagccgtt ttagccagac aatgatgtgg atcgacacgg
    37081 ttaacggttt gattatggtg gactgcgcca gtgccaaaaa agcggaagat acgctggcat
    37141 tactgcgtaa aagcctgggg tcgttaccgg ttgtaccatt gagcatggaa aacccgattg
    37201 aactgacgct gaccgaatgg gttcgctccg gtagtgcggc acagggcttc cagctgcttg
    37261 atgaagccga gctgaaatcg ttgctggaag atggcggcgt gatccgcgcg aagaaacaag
    37321 atctgaccag cgaagagatc accaatcaca ttgaagccgg aaaagtggtg actaaactgg
    37381 cgctcgactg gcagcagcgc attcagtttg tgatgtgcga cgatggttcg ctcaagcgtc
    37441 tgaagttctg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa gatttcgccc
    37501 agcgttttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta attcaaaacc
    37561 tgattgaagg attaggtggc gaagcacaac gttaattgct gattttcctt taatgccgga
    37621 tgcgacgcct gccgcgtctt atccggcgta cgaagccaca ccaggcatat aattattcgc
    37681 tacggcgggc aataattttt agcgcagcaa tattatgcgt tttatgctgt aacttgctcc
    37741 atggacgttg tgtcattgtt tttcctcaag ccagtgacgc gcttcactgc gaattttccg
    37801 cattgccccg gttttcacta aatcccgtaa cagcgaaaga tcgggttgtt cgacaaataa
    37861 gagtaatgat ggatcctctt ttaaccacgt cgttttcaca tcggcagcga gctgtggatt
    37921 attcatagct agcgaccgat gccgagactc ggtcaatgtc gttaacaacg acgccggggt
    37981 atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca gcaatgcacg
    38041 ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaacaa tatctggaga
    38101 tcttcactga tatttatatc aaccttgatg aattatgaat ataccggaaa ttgaaaaaat
    38161 cgcagcaaag gaaatattat tatgggttaa agagaggata tttcagaaaa atgccctccc
    38221 gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg gttcggcaac
    38281 ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt catacacctg
    38341 ccagtcggtc gcgtcaggca gtaacacatt cagcgcgcca ctgattactg tcatctcttc
    38401 cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc tggcgcgacc
    38461 agtgctgctg ctggaaaagc cgattgattt cactttgccg gaaaagtact cattactttg
    38521 aagcataaac tggccctgtc tggtagttga acatctactt cactataggg gccagaggcg
    38581 ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat caatacgtta
    38641 gacagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg ttggtgaaat
    38701 cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt cccggcatca
    38761 atgatttttt gttctgaatc atgaatgggg ttgcccaatg aaaataccgg cgatttctgc
    38821 aaaatttgcc atttttgcgc ctgaacggtc agcatctcct caaccggaac gataaccccc
    38881 acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga cggctcaaga
    38941 aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa taaaatgacg
    39001 tcataaccct gattatcgag cacttcaacc acaccttgca ggtctcgctc cactttacga
    39061 cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc ttcgcctgct
    39121 tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcaa gctatgatgg
    39181 gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg cataggtaca
    39241 atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta cttttctttc
    39301 gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt gctacaaatg
    39361 acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat gattccagta
    39421 atggattgtt attgtttaat attctaatta ttagaaaaac atgaattatg aaaaatgtga
    39481 cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc ggtcactctt
    39541 tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagccgtc ggatgttcac
    39601 tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt accgtctgtc
    39661 ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc ggtttcgttg
    39721 gcatcgttct tccttatttc acgggatgaa cgttaagtat aggcgctcta aaatcaacaa
    39781 ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg ttcgtttgtt
    39841 gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc cagcacttcc
    39901 tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc ttccggtgca
    39961 gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac gatcccgtta
    40021 ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt agcgataacg
    40081 gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa tcccgcccac
    40141 tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa ccactgatcg
    40201 gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt tgttttacca
    40261 cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc ccaataggtc
    40321 gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat gccatcaaac tagtacaatt
    40381 tcgattgtaa agaaaaaatt ccacttaaag tgaaaatctc aatacatccc ttactatacc
    40441 aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga ctacgttgca
    40501 cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt tgtgaaaggc
    40561 tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa tcgcgcatcg
    40621 tcaggcgctc acgaatggtc gctggcgtgt aacgttcgcc gcgcggatta agcgccgctg
    40681 cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc aaatcgcccg
    40741 cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg acgcgcagcg
    40801 tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt accagcggca
    40861 tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga cacgcgtcgg
    40921 catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt gtgtcgcgct
    40981 tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa caatgaattt
    41041 cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg caatatggga
    41101 aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca aatctgggta
    41161 tacaccccct ccccggataa agtcgccgcc ctgcatgacc agttcggcat caacgccgca
    41221 gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt taaacctggc
    41281 atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc tctggtcgtt
    41341 tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca tgaccggaaa
    41401 attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac ctccgtaacg
    41461 ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt ccgctgcttt
    41521 ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tggtcggtgt gagcggttct
    41581 tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt gctgggcggg
    41641 atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc cgcaaaaatg
    41701 gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc accgggaggc
    41761 accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc agtgatcgaa
    41821 gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg actttcgccg
    41881 gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg taaagcgcca
    41941 aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc ggcgcaaccc
    42001 ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg tttagccctt
    42061 cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac atgattactg
    42121 caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta atttgtaact
    42181 gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta tcgttgatgc
    42241 tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc cgccgacagt
    42301 tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg cccgtcatgc
    42361 cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg gttgccgtct
    42421 ggtagctgac ccagccaaac agcagaggat aaacgacaat aatgggaagg gagagccacc
    42481 attccagcgg cgcactattg aacgacacgg taatgcccgt aagctcgagg gtgacaaggc
    42541 aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct gcccccatca
    42601 aattcagaca cataatcatc aacatagcgg tggaaggcag cacgtttacg cccattacgc
    42661 ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc cggctaagcg
    42721 gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac gcccagccga
    42781 ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa gcaatcggta
    42841 agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg aagcgcagcc
    42901 cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc gccgccgctt
    42961 ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa ttttcccaaa
    43021 ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact ttcgtagctc
    43081 acccgttagc actatcaggt atcaggcggc tttcttgagg caggcactca taaacttatt
    43141 acgatcatca cctttcagag attgttgcgt tgcttgatta ttacattcgc gcatcttttg
    43201 ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca ggcaatcact
    43261 catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct gattacagga
    43321 tgtcatacgc tgttgttgtg gggttaaggt tctctcggca gcgccgacgg tggttaaaaa
    43381 aaccagacca aaaagcaagg taaccagtaa tgttattttc atagcaccat ccctcttcat
    43441 gttttaacca tgagcgtatg cgcccgtgat ctgccattaa gtctggttgc taacagcaaa
    43501 aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc cccagggcgg
    43561 ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacaacattg gcggcatgcg
    43621 ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc tcttcggagt
    43681 tcccgtagct catcaccatc actgcgccat ctttggtatt tagcgcctgg gtgaggcccg
    43741 gagctttggt atccggcgcg acaatctggc tggcgtgggc gtgatcagcg gtgactatga
    43801 ccagcgtgtt gccatccttt ttagcgaatt ccagcgcccg ttgtacggct tcatcgagat
    43861 cgaccgtctc gccaatttgc ccacaaggat tcgcagcgtg atcctgttta tcgattgacg
    43921 caccttcaac ttgcaggaaa aatcctttct catttttact caacaattca atggctttgt
    43981 cggtcatctg cgccagggtt ggtacactgt cattacgttg cggattaggc gtacaggtga
    44041 ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg tcctagccag cgcactggca
    44101 tattgccgtc agcaaacagt ccaagcagag gtttttgctg attcgcttcc gtcaccgaat
    44161 tcagtgaggc agcatcgctg accaactgat aaccacgcgc ctgtgcctgt tcacgcagcg
    44221 tttttccctg ccattcaccg gcggttgccg tttcagcaaa ggtttttgcg ccaccgccaa
    44281 gcgtaacgtc ggcacgagcg ttaagcagct gttcggtaat cgatcctttt ccgccttttt
    44341 ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat ttgcgcgagg
    44401 tcacatgcgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta gaaacgttac
    44461 cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct ttttcgtgaa
    44521 tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg gttgctgatg
    44581 cagccgaatc ggtgacgtag tccggtttgc cggttttttt attcagcgca tagtgagtgt
    44641 attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca ccttcggcat
    44701 aattacgtgc ggctgtaatt tccgaatccc ccatcccatc gccaatcagc aaaataatat
    44761 tttttgcagg tttatcgcta agagaatcac gcagagcggc agtctgatca cccgttaaac
    44821 ggcgagcacc gccggatgca gtaatatcgc cctgagcagc ccggttttcc agaacaggca
    44881 tttctggtgt ccgggctttt gtcacagggg taaacagtaa cgataagagt gccagtgcaa
    44941 tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat aaaaacaaag
    45001 cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt aactttttac
    45061 tgacgaggat gcttcaataa cttctttacg taatcgcgca gcagctccgt atcgtcgtca
    45121 ggaatgctgg catcgggctt tacctcgtac agcgccccct ctacctgatc aatcaagcgc
    45181 tgttggtcat tttgcgccat attgcgaagc attgcagtga cgataatctc caaagcttct
    45241 acctgcgcac acagttcttt cgactcttct tctttttggg caagcttaaa taacaactca
    45301 gcaatgagat ttttcatgtc tgtatttcct tatccaaagt atggagaagt taacattatg
    45361 tttcacaact tcatagtgtc tggtagtatt atttttatga caaaggaaat actttgcgca
    45421 aatatttttt gtttgattac cagcgaaacg tttctcttat ttaaataatt acccacacaa
    45481 aatataggct tttaattatc aacctttatt ataaataaaa gttatgaaat atattctctt
    45541 cgttaactta taatttagag aaaaaaactc accgcatggc catttaagcc aggaataaat
    45601 tagcttcccg ttcatcattt ttgtgatctt gcgcacatct ttgagataac gcactttctt
    45661 caccagtacg ttgcacgctt tgtcagcaga agattgcaac aatctttcag aaggccgcga
    45721 atcccccgtt ttgtcgtggt aatgtatgca ctttgtgtta gacgggatag gtttttaaga
    45781 tggaaaaact gcgggtagga atcgtttttg gtggtaaatc agcggaacat gaagtgtctc
    45841 tgcaatcggc aaaaaacatt gtcgatgcca ttgataaaag tcgcttcgac gttgtgctgc
    45901 tgggcattga taaacaaggg caatggcacg tcagcgatgc cagcaattat ctgctaaatg
    45961 cagacgatcc tgcccatatt gcgttacgcc cttcggcgac cagccttgcg caggtgccag
    46021 gtaaacatga gcatcagctt atcgacgcgc aaaacggtca gccgttgccg acggtggatg
    46081 tcattttccc gattgtccac ggtacgctgg gcgaagatgg ttccttgcag ggaatgctgc
    46141 gggtcgccaa tttaccgttc gtaggttctg atgttctggc ttcagcagcc tgtatggata
    46201 aagatgttac caaacgtctg ctgcgcgatg ccgggctgaa cattgcgcca tttattaccc
    46261 tgacgcgcgc caatcgtcac aacattagtt ttgccgaagt ggagtctaaa ctggggttac
    46321 cactgtttgt aaaaccggct aatcagggct cttctgttgg tgtcagcaaa gtaaccagtg
    46381 aagaacagta cgcaattgcc gtcgatctgg cgttcgaatt cgatcataaa gtgatcgttg
    46441 agcaagggat caaaggtcgt gagatcgaat gcgcagttct gggcaacgac aacccgcaag
    46501 ccagcacctg tggcgagatc gtactcacca gcgacttcta tgcctacgac accaagtaca
    46561 ttgacgaaga tggcgcgaaa gtggtagttc cggcagccat tgcgccagaa atcaacgata
    46621 agatccgggc gattgccgtt caggcttatc aaacgttggg atgcgcaggc atggcgcgtg
    46681 tagacgtgtt tttaacccca gaaaacgaag tggtgatcaa cgagatcaac acactgcctg
    46741 gcttcaccaa catcagtatg tatccgaagc tgtggcaagc cagcggtctg ggttacaccg
    46801 atctgatcac acgtttgatt gagctggcgc tggagcgtca cgctgcggat aacgcattga
    46861 aaaccacaat gtaatatttt aacgcccgat aatattctta ttgggcgttt tcagtttctt
    46921 catcgcgacg acgaataatc agccccgcca gccagaaact aatcacccac gtcaccagac
    46981 cgacggcata ggtctgccaa ccttttgctt caaatcccag taaccccacc acgccattaa
    47041 gaatgaatat aaggccaatg gcgaacgcat agtagtgcca gtcacggcgg atttttacag
    47101 gcagattcat ggcagctccg ttaacgaaaa acaacatcct tgcatattct gtaacaaccg
    47161 tctgtggtgt agacaacatt ttcgtaattg ttaataaatt taacttaagg ttgccatttt
    47221 gtgactcaca acgaagattc acgcttcggg aatattccca acatcaattt accatgattc
    47281 tgatattgac aatctaaatg acctgtcaga cttgccataa gccgctggta tcacaccaga
    47341 agagaagcag atgacttccg gaggttttta tggctgattt caccctgtca aaatcgctgt
    47401 ttagcggaaa atatcgcaat gcctcttcaa cgcctggcaa cattgcctat gcgttgtttg
    47461 tgctgttttg cttttgggct ggggcgcaat tgctgaacct gttagtgcat gcgcccggcg
    47521 tctatgagcg tttaatgcag gtccaggaaa caggtcgccc acgggtggaa attggtttag
    47581 gtgtcggcac cattttcggg ctgatcccgt ttttagtagg ctgcctcatt tttgcagtgg
    47641 tggcgctatg gctgcactgg cgacatcgcc gccagtaaga tttacgttgc cagatacggc
    47701 ctaaaacgcc ttatctggca tgacattcct gtttgatcac tgtttcatac aacgcgcccg
    47761 ccgttcgtct acgcgcttcg caaaccaggc cgtagtcagg ttgcgggtga ttttcgggct
    47821 ttccagttga atgccaggca acatttcgcg gggtagtgat ttacccgttt tcgcttcggc
    47881 aagttgataa actttcttgt acagcgccgt ctcctcaaaa gagaagctgt cacctttctc
    47941 taactgacgg cgaatttcgc tgtcgctcat ccccagtttt cccgccagtt tgcgcgttgc
    48001 cagttcggtt ttcccgggtt ctttactgtc gtaacgaatt aaatcaccgt ccagtgccag
    48061 cttcacgccg ctggccttac tgactgcgtt ctgaaacgcg gcattacggc tggcgtacca
    48121 gccagcgtta aaatcagcaa agcggtatat cggtgcgcta tagctggcgg gataattcag
    48181 taaatggtaa gtaccaaacc acaacccgcc gcggcgactg aagacttcct gacgaactgt
    48241 accgtccatt ttccacggat accctttggt atgctgttcg gcaaaagcaa tgctgacctg
    48301 catcggccca ccggtgcgca caggattaag cgaaccaaac agcgtctgcc ccattggtac
    48361 catgctgatc agatcgtcaa aaatcgcgct caattgcttt tctgtacgca ccgaatctaa
    48421 ccgttcgcta taacttttgc cgtttggcga tttgattttt agcgcggtat gaaccagaaa
    48481 agcaggaatg tgcatccgtt cagcacgacg gtcaatctct tgccaggcga tcttgcttaa
    48541 gcccggaacg gccggatctg cctgataatt tgactcctgc tgcgccaccg ccagcaccga
    48601 gcagacgttt tccagcgtcg gtgccagccc ctgactttca aaggtggtgg cgagatcttt
    48661 cgcccaggca tcgcggtctt tcacgctcgc aggcatcttc tgccgcacga cgctcgctac
    48721 atctatcgct ttttcgccct ttttcaacgg ttgcggagcc tgactactac accccgctaa
    48781 aaccagaacc gccaacaatg acagcgaaga tagatgattg acgcgcgaca ttacttctcc
    48841 tttttagctc aaggtatggg ttacttcctg aattttgtca ccatccagct catgttcaaa
    48901 gctgcgcaga cgtttgtaga tagacatcaa ctcaaccagt gtggtccatg agttaatcag
    48961 gtactggaaa gcaccgcgaa cctgaccaaa aacgttggta atctgcgtca tcaggccgag
    49021 cgtaatcgta ccggcaacaa ttgacggaaa cagcaagaac aaaccgaaaa cgttatcgac
    49081 ctgcaaatag agaatgcggg cgatgttgaa atacatatag tgaaaataga ggcgaaaata
    49141 gtttttccgt acggcgctaa acagctcgcg taccgtaggc ggcgtcgcgc gcgtggcatc
    49201 gtcttcacca taaaccagct ctttacggta ggcggcctct actcgctggt ttttaaactc
    49261 cagccccggc agtttgatcc ctaccactgc cagcaatccg gtccccatca gcgaccagac
    49321 gatagcggca atcaccagac catacggaat gtgcccgata atcggcagct ccggcacatg
    49381 cgcggagagc gttaccagca ccggcagaaa ggcgatcaac gtcatgatgg cgttgataaa
    49441 actgacgccc atattctcca gcgttgaagc aaaacgcatg gtgtcttcct gcacacgctg
    49501 tgcggcacct tcgatatgac gcagttgttg ccagttcgcc atgtaatatt cgttcatcgc
    49561 cgtacgccag cggaacacat agtgactgac aaagaagttg ttcagtacac tgatcaccac
    49621 cgcgatcagc gcaatcccca gaaagacgcc cacttcgcgg taaaattgtt cgatagtgac
    49681 tttatgcggc gaacttagcg cggtttgaat cagatcatag aacggtgcat actaggcgtt
    49741 gacggcgacc ccgacttcca ccaaaaacca ggtgacgaag atgatcagtg cagtaccgag
    49801 tattgaccag tattgccaac ggtgcgggct gtagataaac cagaaaaatg caaaaagtcc
    49861 tacgcaaaca atgtagtaag cgtaaaaaat caggaaatcc aacgaccaga aacgcgcggc
    49921 gctaatcggg atctgcccgg aagcgccggt gatacgcgcc acccagtcac ccccaccggc
    49981 ttgccagaag ataacggcaa tcaatgccca aacaaaggcc gagagaaaaa acgttcccgg
    50041 ctttgggaaa aaagacttaa acatatgcac tcctgctaac ttcttatcgt tttaacaact
    50101 gctgtgtatt aaccgcatga ccgctacaaa gcgtcagcca aatgaaaccg cccattgtga
    50161 cccatagcaa ggcgcttagt tcgccagcct gaccataaga attgtttcga cggatttcac
    50221 aaaccaaaga ttgtttacat aagcggatta acacgcgcag gatcacaaat gcaggcattg
    50281 gtgagcagga aaaagatcgt gtttacgcgc cgttacgttt ttaacactat tattgcacta
    50341 attcgccctt tgcaatccat caatgagtag tataaatacg ctcagttacc ttcattcaat
    50401 ctatggacac caccgttgaa acgtagtctg cttttttctg ccgtgctgtg tgcggcgtca
    50461 ttgacctccg tccatgcggc gcagccgatc actgaaccgg agtttgcctc tgatattgtc
    50521 gatcgttatg ccgatcatat tttttacggc agcggtgcca cggggatggc gctggttgtt
    50581 atcgacggta accagcgcgt ctttcgcagt tatggcgaaa cgcgacctgg taataacgtt
    50641 cgcccgcagc tggattcggt cgtgcgtatt gcttccctca ccaagctaat gaccagtgaa
    50701 atgctggtga aattgctcga ccaggggacc gtgaagttaa acgatccgct aagtaaatat
    50761 gctccgccag gcgcacgcgt gccaacctac aatgggacgc ccattacgct ggtcaatctg
    50821 gcaacccata ccagcgcgct gccccgtgaa cagcccggtg gcgcggcaca tcgtccggtg
    50881 tttgtctggc caacgcgcga gcaacgctgg aaataccttt ctacggcgaa gctgaaagcc
    50941 gcgccaggca gtcaggcggc gtactctaac cttgcgttcg acctgctggc tgatgcactg
    51001 gcgaatgcgt ccggcaagcc ttatacccag ttgtttgaag agcagattac ccgtccattg
    51061 gggatgaaag acaccaccta caccccctca ccggatcagt gccgccgttt aatggttgca
    51121 gagcgcggtg ccagtccatg caataacacg ctggcggcaa ttggcagcgg cggcgtctat
    51181 tccacgcctg gcgatatgat gcgctggatg cagcagtatt tgtcatctga tttttatcag
    51241 cgcagtaacc aggcagatcg catgcagacg ttgatttatc agcgcgcgca gtttacgaaa
    51301 gtgattggca tggatgtccc cggcaaagcg gatgcgcttg gtttaggctg ggtatatatg
    51361 gtcccgaaag agggtcgtcc ggggattatt cagaagacag gaggtggtgg cggtttcatt
    51421 acctatatgg cgatgatccc gcagaagaat attggcgcgt ttgtggttgt cactcgttcg
    51481 ccgttgacgc gctttaaaaa tatgagcgat ggcatcaatg acctggtgac cgagctaagc
    51541 gggaataaac cgctggttat ccccgcgtcc tgattaatac tctgaaggca aacggttgat
    51601 agcgaccagt ttgcctttat tcatttcgat atagccgcct ttacgcaaag cagcgagaac
    51661 ttctgcgacg acagaacgcg atatgcgtgt acgttgatgg atataattca tcacaccaat
    51721 acgtgatcgc aattcttcat tccagtcaat cattgataat aatgttgcgc ggatttgttc
    51781 gtaggaatta tgcccaatga gctgcacgtc gcgtaattcc agaattctat tttgccaggc
    51841 taaccagtaa aaagcgtctc gccagagttg attttgttca ataagcgtaa tggtttgttt
    51901 ggctggtaga tgatatcccg tacaatttcc ttctgatatt aatttgtatg gtatatcgtt
    51961 tttcattaaa ccatcagcca gccccataat ataaggagcc tgggtaatac cgataagtac
    52021 gttttcttct ctacgcagag agataactcc ctctaaaata acgaaagtat cttcgttatt
    52081 gacatcactg gaaaatataa cttgcttttc attattaaac tcgaagcgcg taccgtatct
    52141 ggacaaacat ttatcgagct taccaaattc ctgaagaggt ttaactacag ataacatttg
    52201 cgcgtccttt gcagtaatgc ccgtcaaatc cttgacgggc attatttaga ttaaattacc
    52261 agaatttctt cggagtgaag aatattacca ggtatattta acacccacgt tcgcggacca
    52321 gtcttgatct acgtcaccac caccgaggta gttagcatcg gtataggcgc tgaagttctt
    52381 ggtgaagctg aactgagtac ccagaccaac acgtaccgca gacccttcag taccgttatc
    52441 gatggaatca ccgttcacat cgttatcgtt gttagagtcg tcgtagacgt aagccagttt
    52501 gaagtacgga gtcagagcct ggtcttcgct gtaggtgaag gtataacctg catctacacc
    52561 cagttcataa cgcatgctgt cgtaagactg accgtcaact ttcatgtcgt tgctcagctg
    52621 gtagtcatca ccagactgga acagaccaga aacgctgccg taaggagtca cgtaaccagc
    52681 atcacccagt ttgaagtcgt aaccggcttt caaaccgaag ccccaagcgt cggagttggt
    52741 gctaccgtca acgtaagtac cgttgctcat ggttgcagac aggtcgttgt tgaagtgaga
    52801 gtagctcaag ctaccatcaa caaagacgtt gttcgcgaag tgagcagaag agtagatgta
    52861 ggcagtctgg ctgtcttgat ccacctgacc agaacggtca ttcatgtcac ctttagcgaa
    52921 gcctgcagcc gcaccgacga tccacttagc gttgttaccg tcaattttgg tatcaacacc
    52981 gaccatgatg ccgttaacat cctgatcata gttgatggtg ccgttgtcgc cgttgaagtt
    53041 accaccgaag tagcttaccc atgcgccgcc gttatcagcc aggccatgac gagagttggt
    53101 cagacgagta ccaacggtgt cttgttccag gttccagata ttggtgttcg cagacgggat
    53161 gctcagcgcc atgttagcgt agtcggtcag ctccatctgt tgcagaacaa cggtgttacc
    53221 gcgctgttca gcctgatagg tgtatgcacc caggtcagct ttgttagcag cagagaaggt
    53281 cgcgttggtg ttgacgtcgt ttacgtagat aatttcttta tctttgtaat cagcgatagc
    53341 gccagcgcct gttgcgttgt cgatacgaac tttgtagtta ccggttgcag caacaacgtt
    53401 gtcaacagag ctgttatcca gttcgtcgcc gttgttcatg tcaccgttac cgttgatagt
    53461 cagatgacca tcagagttca tagcaacaat accgtagccg tagttagact tggaagtatc
    53521 ccaggtacgg tcgttcacca aatcagcgtt cagaacataa tcgctgctgt ggatatcgaa
    53581 tacaccagac actacgttgc cgttatggtc agcgatgttg ccgctggtca gttccaaagt
    53641 atcagtctgg aacggattat catcgaactg gtctacatta atatccagta aaccaccgtt
    53701 agtcacagtt attgtatttg ctaacagcga cggagtgtaa taaccattat agatacttga
    53761 attgtcatca ctagacaacg ctacagtact gtagctatca actgtcagat gatcagttcc
    53821 aacataacct tcatctccaa tgttcaaaga agaaccattg gtcagagcga tgctatcaga
    53881 aatcaagcta gattcagaca cattaacctg agagccatta ttcacagcta aagtatcaat
    53941 gtttgaagcg ttaacggtat tccattcaga accattattc agagtgacgt taaacacacc
    54001 gctctggaat acttgatcat caatcgtacg accccaagca tttgcttctg ttacaacgcc
    54061 cggatacagg ctattacctg taacatcata ccagtcagtc gaagcagtag attcatcatc
    54121 aacatttgct gatgtagtag ctgagcctac ccacttagaa ccattatcca gagtgatatt
    54181 cagttcatca acattaagtg agtcatcagc ccagccacca ttggtgtcca agtacgccat
    54241 cggcattact gtcatgacct tgaggataga agtttgcgtt gaaagtactt gcgaaaacaa
    54301 catcacccag cagagtagaa ttgctgaaat aaacattgtt tttcatggag taatttgaag
    54361 ccgcactatc actgaaatac agagctacat cattagcttc gccgttacca gagtaatcac
    54421 taggttccgc agagttacca tagaaaccac tgtcttccag agtagttaca gaacctgaag
    54481 tcactgtgga atcggtaact gagatattgt tatcccaagt atgagattca gtagtgtaag
    54541 tattgtttac ggtattaccc tgagtcaggc tgataccagc aacgcgggag ttgttagaaa
    54601 tattgatgtt actttcgaca tcaagattca ctgccagccc aaggccaaca ccaccgtttg
    54661 caaacaaacc ggcgtattct tcaccttcac cagctggggt atggaaggtt tcgttagtat
    54721 atttggtaac gtcaccgtcc agatatgaat cagtgaagta taaaccttca taatcgtcat
    54781 caattgtaga attagcaata tttaaagtga atacatcacc atcatgccaa ttatcatcaa
    54841 ttacacgatc agtaccgtat tcagagaact gatagacata atgaccatca ctgctttcac
    54901 ccataggcag cattgaagtg attgaaccat gaatcaggga gttgctgatg ttcaactggt
    54961 ttgttgttcc atcagtaaag ttgatcctac ccacgtaata tggacacagg cctaagcgag
    55021 gttcttgttt tcaaattgtt ccggactgag gccgccacac aaactgtgcc gccgccaccg
    55081 attgtaatca cattcgatat aattaaatac cgttgcccgc attatttccc ggctgataaa
    55141 gtgttctcca tggatacatt ccactttcag cgaatgaaag aagctttcca cgcaggcatt
    55201 atcgtagcag caaccttttg cgctcatact tccacgcaga ttatgccgct tcagttgcgc
    55261 ctgataatct gctgaacagt actggcctcc acggtccgtg tgaacgataa cgttccgggg
    55321 cctcttacgc caccacagcg ccatctgcag ggcatcgcag gccagttgcg ccgtcatgcg
    55381 tggcgacatt gaccagccaa taacggcacg tgac
//
LOCUS       DAZKGS010000012        53408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKGS010000012 DAZKGS010000000
VERSION     DAZKGS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..2689
                     /gene="malP"
                     /locus_tag="ACHOQY_000759"
     CDS             296..2689
                     /gene="malP"
                     /locus_tag="ACHOQY_000759"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9676906.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2699..4783
                     /gene="malQ"
                     /locus_tag="ACHOQY_000760"
     CDS             2699..4783
                     /gene="malQ"
                     /locus_tag="ACHOQY_000760"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9676907.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4828..6144)
                     /gene="gntT"
                     /locus_tag="ACHOQY_000761"
     CDS             complement(4828..6144)
                     /gene="gntT"
                     /locus_tag="ACHOQY_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9676908.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKILADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6505..7080)
                     /gene="nfuA"
                     /locus_tag="ACHOQY_000762"
     CDS             complement(6505..7080)
                     /gene="nfuA"
                     /locus_tag="ACHOQY_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9676909.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7139..7822)
                     /gene="gntX"
                     /locus_tag="ACHOQY_000763"
     CDS             complement(7139..7822)
                     /gene="gntX"
                     /locus_tag="ACHOQY_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9676910.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7860..8630
                     /gene="bioH"
                     /locus_tag="ACHOQY_000764"
     CDS             7860..8630
                     /gene="bioH"
                     /locus_tag="ACHOQY_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9676911.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8659..9537)
                     /gene="rpnA"
                     /locus_tag="ACHOQY_000765"
     CDS             complement(8659..9537)
                     /gene="rpnA"
                     /locus_tag="ACHOQY_000765"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP9676912.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(9740..9976)
                     /gene="feoC"
                     /locus_tag="ACHOQY_000766"
     CDS             complement(9740..9976)
                     /gene="feoC"
                     /locus_tag="ACHOQY_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9676913.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(9976..12297)
                     /gene="feoB"
                     /locus_tag="ACHOQY_000767"
     CDS             complement(9976..12297)
                     /gene="feoB"
                     /locus_tag="ACHOQY_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9676914.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12314..12541)
                     /gene="feoA"
                     /locus_tag="ACHOQY_000768"
     CDS             complement(12314..12541)
                     /gene="feoA"
                     /locus_tag="ACHOQY_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9676915.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(12979..15300)
                     /gene="yhgF"
                     /locus_tag="ACHOQY_000769"
     CDS             complement(12979..15300)
                     /gene="yhgF"
                     /locus_tag="ACHOQY_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9676916.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15397..15873)
                     /gene="greB"
                     /locus_tag="ACHOQY_000770"
     CDS             complement(15397..15873)
                     /gene="greB"
                     /locus_tag="ACHOQY_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9676917.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            16101..16820
                     /gene="ompR"
                     /locus_tag="ACHOQY_000771"
     CDS             16101..16820
                     /gene="ompR"
                     /locus_tag="ACHOQY_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9676918.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            16817..18169
                     /gene="envZ"
                     /locus_tag="ACHOQY_000772"
     CDS             16817..18169
                     /gene="envZ"
                     /locus_tag="ACHOQY_000772"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9676919.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(18245..19867)
                     /gene="pckA"
                     /locus_tag="ACHOQY_000773"
     CDS             complement(18245..19867)
                     /gene="pckA"
                     /locus_tag="ACHOQY_000773"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9676920.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20246..21970
                     /gene="yhgE"
                     /locus_tag="ACHOQY_000774"
     CDS             20246..21970
                     /gene="yhgE"
                     /locus_tag="ACHOQY_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9676921.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(22105..22983)
                     /gene="hslO"
                     /locus_tag="ACHOQY_000775"
     CDS             complement(22105..22983)
                     /gene="hslO"
                     /locus_tag="ACHOQY_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9676922.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(23008..23409)
                     /gene="hslR"
                     /locus_tag="ACHOQY_000776"
     CDS             complement(23008..23409)
                     /gene="hslR"
                     /locus_tag="ACHOQY_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9676923.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(23420..24088)
                     /gene="yrfG"
                     /locus_tag="ACHOQY_000777"
     CDS             complement(23420..24088)
                     /gene="yrfG"
                     /locus_tag="ACHOQY_000777"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9676924.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(24153..26288)
                     /gene="igaA"
                     /locus_tag="ACHOQY_000778"
     CDS             complement(24153..26288)
                     /gene="igaA"
                     /locus_tag="ACHOQY_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9676925.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            26608..27168
                     /gene="nudE"
                     /locus_tag="ACHOQY_000779"
     CDS             26608..27168
                     /gene="nudE"
                     /locus_tag="ACHOQY_000779"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9676926.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(27334..29886)
                     /gene="mrcA"
                     /locus_tag="ACHOQY_000780"
     CDS             complement(27334..29886)
                     /gene="mrcA"
                     /locus_tag="ACHOQY_000780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9676927.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            30006..30785
                     /gene="hofM"
                     /locus_tag="ACHOQY_000781"
     CDS             30006..30785
                     /gene="hofM"
                     /locus_tag="ACHOQY_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP9676928.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            30785..31324
                     /gene="hofN"
                     /locus_tag="ACHOQY_000782"
     CDS             30785..31324
                     /gene="hofN"
                     /locus_tag="ACHOQY_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9676929.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            31308..31748
                     /gene="hofO"
                     /locus_tag="ACHOQY_000783"
     CDS             31308..31748
                     /gene="hofO"
                     /locus_tag="ACHOQY_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9676930.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            31738..32142
                     /gene="hofP"
                     /locus_tag="ACHOQY_000784"
     CDS             31738..32142
                     /gene="hofP"
                     /locus_tag="ACHOQY_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9676931.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            32054..33292
                     /gene="hofQ"
                     /locus_tag="ACHOQY_000785"
     CDS             32054..33292
                     /gene="hofQ"
                     /locus_tag="ACHOQY_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9676932.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            33693..34214
                     /gene="aroK"
                     /locus_tag="ACHOQY_000786"
     CDS             33693..34214
                     /gene="aroK"
                     /locus_tag="ACHOQY_000786"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9676933.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            34271..35359
                     /gene="aroB"
                     /locus_tag="ACHOQY_000787"
     CDS             34271..35359
                     /gene="aroB"
                     /locus_tag="ACHOQY_000787"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9676934.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            35451..36737
                     /gene="damX"
                     /locus_tag="ACHOQY_000788"
     CDS             35451..36737
                     /gene="damX"
                     /locus_tag="ACHOQY_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9676935.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            36844..37680
                     /gene="dam"
                     /locus_tag="ACHOQY_000789"
     CDS             36844..37680
                     /gene="dam"
                     /locus_tag="ACHOQY_000789"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9676936.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            37698..38375
                     /gene="rpe"
                     /locus_tag="ACHOQY_000790"
     CDS             37698..38375
                     /gene="rpe"
                     /locus_tag="ACHOQY_000790"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9676937.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            38368..39126
                     /gene="gph"
                     /locus_tag="ACHOQY_000791"
     CDS             38368..39126
                     /gene="gph"
                     /locus_tag="ACHOQY_000791"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9676938.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            39119..40123
                     /gene="trpS"
                     /locus_tag="ACHOQY_000792"
     CDS             39119..40123
                     /gene="trpS"
                     /locus_tag="ACHOQY_000792"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9676939.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            40392..41297
                     /gene="yhfZ"
                     /locus_tag="ACHOQY_000793"
     CDS             40392..41297
                     /gene="yhfZ"
                     /locus_tag="ACHOQY_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP9676940.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            41314..41676
                     /gene="yhfY"
                     /locus_tag="ACHOQY_000794"
     CDS             41314..41676
                     /gene="yhfY"
                     /locus_tag="ACHOQY_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9676941.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            41760..42923
                     /gene="yhfX"
                     /locus_tag="ACHOQY_000795"
     CDS             41760..42923
                     /gene="yhfX"
                     /locus_tag="ACHOQY_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP9676942.1"
                     /translation="MFVEALKRQNPALISAVLSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            42923..44149
                     /gene="yhfW"
                     /locus_tag="ACHOQY_000796"
     CDS             42923..44149
                     /gene="yhfW"
                     /locus_tag="ACHOQY_000796"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9676943.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            44146..45024
                     /gene="php"
                     /locus_tag="ACHOQY_000797"
     CDS             44146..45024
                     /gene="php"
                     /locus_tag="ACHOQY_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP9676944.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            45035..45388
                     /gene="yhfU"
                     /locus_tag="ACHOQY_000798"
     CDS             45035..45388
                     /gene="yhfU"
                     /locus_tag="ACHOQY_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP9676945.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            45400..46704
                     /gene="yhfT"
                     /locus_tag="ACHOQY_000799"
     CDS             45400..46704
                     /gene="yhfT"
                     /locus_tag="ACHOQY_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP9676946.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            46716..47801
                     /gene="yhfS"
                     /locus_tag="ACHOQY_000800"
     CDS             46716..47801
                     /gene="yhfS"
                     /locus_tag="ACHOQY_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9676947.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(47953..48684)
                     /gene="frlR"
                     /locus_tag="ACHOQY_000801"
                     /gene_synonym="yhfR"
     CDS             complement(47953..48684)
                     /gene="frlR"
                     /locus_tag="ACHOQY_000801"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP9676948.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(48784..49569)
                     /gene="frlD"
                     /locus_tag="ACHOQY_000802"
     CDS             complement(48784..49569)
                     /gene="frlD"
                     /locus_tag="ACHOQY_000802"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP9676949.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(49566..50396)
                     /gene="frlC"
                     /locus_tag="ACHOQY_000803"
     CDS             complement(49566..50396)
                     /gene="frlC"
                     /locus_tag="ACHOQY_000803"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP9676950.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(50446..51468)
                     /gene="frlB"
                     /locus_tag="ACHOQY_000804"
     CDS             complement(50446..51468)
                     /gene="frlB"
                     /locus_tag="ACHOQY_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP9676951.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(51489..52826)
                     /gene="frlA"
                     /locus_tag="ACHOQY_000805"
     CDS             complement(51489..52826)
                     /gene="frlA"
                     /locus_tag="ACHOQY_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP9676952.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(53121..53288)
                     /gene="yhfL"
                     /locus_tag="ACHOQY_000806"
     CDS             complement(53121..53288)
                     /gene="yhfL"
                     /locus_tag="ACHOQY_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9676953.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        12564 a        13488 c        14686 g        12670 t
ORIGIN      
        1 attttactta tgaaactgtt tcactccttt acttaattta tagagttacc ttccgctttt
       61 tgaaaatacg caacggccat tttttgcact tagatacaga ttttctgcgc tgtattgcat
      121 tgatttgctg ctaatcctgt ggtttgcact agctttaagt ggttgagatc acatttcctt
      181 gctcatcccc gcaactcctc cctgcctaat cccccgcagg atgaggaagg tcaacatcga
      241 gcctggcaaa ctagcgataa cgttgtgttg aaaatctaag aaaagtggaa ctcctatgtc
      301 acaacctatt tttaacgata agcaatttca ggaagcgctt tcacgtcagt ggcagcgtta
      361 tggcttaaat tctgcggctg aaatgactcc tcgccagtgg tggctagcag tgagtgaagc
      421 actggccgaa atgctgcgtg ctcagccatt cgccaagccg gtggcgaatc agcgacatgt
      481 taactacatc tcaatggagt ttttgattgg tcgcctgacg ggcaacaacc tgttgaatct
      541 cggctggtat caggatgtac aggattcgtt gaaggcttat gacatcaatc tgacggacct
      601 gctggaagaa gagatcgacc cggcgctggg taacggtggt ctgggacgtc tggcggcgtg
      661 cttcctcgac tcaatggcaa ctgtcggtca gtctgcgacg ggttacggtc tgaactatca
      721 atatggtttg ttccgccagt cttttgtcga tggcaaacag gttgaagcgc cggatgactg
      781 gcatcgcagt aactacccgt ggttccgcca caacgaagca ctggatgtgc aggtagggat
      841 tggcggtaaa gtgacgaaag acggacgctg ggagccggag tttaccatta ccggtcaagc
      901 gtgggatctc cccgttgtcg gctatcgtaa tggcgtggcg cagccgctgc gtctgtggca
      961 ggcgacgcac gcgcatccgt ttgatctgac taaatttaac gacggtgatt tcctgcgtgc
     1021 cgaacagcag ggcatcaatg cggaaaaact gaccaaagtt ctctatccaa acgacaacca
     1081 tactgccggt aaaaagctgc gcttgatgca gcagtacttc cagtgtgcct gttcggtagc
     1141 ggatattttg cgtcgccatc atctggcggg gcgtaaactg cacgaactgg cggattacga
     1201 agttattcag ctgaacgata cccacccaac tatcgcgatt ccagaactgc tgcgcgtgct
     1261 gatcgatgag caccagatga gctgggatga cgcctgggcc attaccagca aaactttcgc
     1321 ttacaccaac cataccctga tgccagaagc gctggaacgc tgggatgtga aactggtgaa
     1381 aggcttactg ccgcgccata tgcagattat taacgaaatt aatactcgct ttaaaacgct
     1441 ggtggagaaa acctggccgg gcgatgaaaa agtgtgggcc aaactggcgg tggtgcacga
     1501 taaacaagtg catatggcga acctgtgtgt ggttggcggt ttcgcggtga acggtgttgc
     1561 ggcgctgcac tcggatctgg tggtgaaaga tctgttcccg gaatatcacc agctatggcc
     1621 gaacaaattc cataacgtca ccaacggtat taccccacgt cgctggatca aacagtgcaa
     1681 ccctgcactg gcggctctgt tggataaatc actgcaaaaa gagtgggcta acgatctcga
     1741 tcagctgatc aatctggaaa aattcgctga tgatgcgaaa ttccgtcagc aatatcgcga
     1801 gatcaagcag gcgaataaag tccgtctggc ggagtttgtg aaagttcgta ccggtattga
     1861 gatcaatcca caggcgattt tcgatattca gatcaaacgt ctgcatgagt acaaacgcca
     1921 gcacctgaat ctgctgcata ttctggcgtt gtacaaagaa attcgtgaaa acccgcaggc
     1981 tgatcgcgta ccgcgcgtct tcctcttcgg cgcgaaagcg gcaccgggct actacctggc
     2041 gaagaatatt atctttgcga tcaacaaagt ggctgacgtg atcaacaacg atccactggt
     2101 tggcgataag ttgaaggtgg tgttcctgcc ggattattgc gtttcggcgg cggaaaaact
     2161 gatcccggcg gcggatatct ccgaacaaat ttcgactgca ggtaaagaag cttccggtac
     2221 cggcaatatg aaactggcgc tcaatggtgc gcttactgtc ggtacgctgg atggtgcgaa
     2281 cgttgaaatc gccgagaaag tcggtgaaga aaatatcttt atttttggtc ataccgtgga
     2341 acaagtgaag gctattctgg ccaaaggcta cgacccggtg aaatggcgga agaaagataa
     2401 ggtgctggac gcagtattga aagagctgga aagcggtaaa tacagcgacg gcgataagca
     2461 tgccttcgac cagatgctgc acagtatcgg caaacaaggc ggcgatccgt atctggtgat
     2521 ggcggatttc gcagcctatg ttgaggcaca aaagcaggtg gatgtgctgt accgcgacca
     2581 ggaggcctgg actcgcgcgg cgatcctcaa taccgcccgc tgcggtatgt ttagctcgga
     2641 tcgctctatt cgcgattatc aggctcgtat ctggcaggca aaacgctaag gaagctcgat
     2701 ggaaagcaaa cgtctggata atgccgcgct ggcggcgggg attagcccca attacatcaa
     2761 tgcccacggt aaaccgcagt cgattagcgc cgaaaccaaa cggcgtttgc ttgacgcgat
     2821 gcatcaacgt accgccacga aagtggcggt aacgccagtc ccgaatgtca tggtttatac
     2881 cagcggcaaa aaaatgccga tggtggtgga gggcagcggc gaatatagct ggctgctgac
     2941 caccgaagaa ggaacacagt acaaaggcca tgtaacgggg ggcaaagcgt tcaatctacc
     3001 gacgaagctg ccggaaggtt atcacacgct gacactcacc caggacgacc agcgcgcgca
     3061 ttgccgggtg attgtcgccc cgaaacgctg ttacgaaccg caggcgttgc tgaataaaca
     3121 aaagctgtgg ggtgcctgcg ttcagcttta tacgctgcga tcggaaaaaa actggggtat
     3181 tggggatttt ggcgatctca aagcgatgct ggtggatgtg gcaaaacgtg gcgggtcgtt
     3241 catcggcctg aacccgattc atgcgctcta tccggcaaat ccggagagtg ccagcccata
     3301 cagcccgtct tctcgccgtt ggctgaatgt gatttatatc gacgttaacg ccgttgaaga
     3361 tttccatctt agcgaagagg ctcaggcctg gtggcagttg ccgaccacgc aacagacgct
     3421 gcaacaggcg cgcgatgccg actgggtcga ttactccacg gttaccgccc taaaaatgac
     3481 agcattacga atggcgtgga aaggtttcgc gcaacgtgat gatgagcaga tgaccgcgtt
     3541 tcgccagttt gttgcagagc agggcgacag cctgttctgg caggcagcct ttgatgcgct
     3601 acatgcccag caagtgaaag aggacgaaat gcgctggggc tggcctgcat ggccagagat
     3661 gtatcagaac gtggattcac cagaagtgcg tcagttctgc gaagaacatc gtgatgacgt
     3721 cgatttttat ctctggttgc agtggctggc ttacagccag tttgccgcct gctgggagat
     3781 aagccagggc tatgaaatgc cgattggctt gtatcgtgat ctggcggttg gcgtagcgga
     3841 aggtggggcg gaaacctggt gtgaccgtga actatattgc ctgaaagcgt cggttggcgc
     3901 gccgccggat atcctcggcc cgttggggca gaactgggga ttaccgccaa tggacccgca
     3961 tatcatcacc gcgcgtgcct atgaaccgtt tatcgagctg ttgcgtgcca atatgcaaaa
     4021 ctgcggcgca ttacgaattg accatgtgat gtcgatgctg cgtttgtggt ggataccgta
     4081 tggcgagacg gcagatcagg gcgcgtatgt tcactatccg gtggatgatc tgctctcgat
     4141 tctggcactc gaaagtaaac gtcatcgctg tatggtgatt ggtgaagatc tcggtaccgt
     4201 accggtagag attgtcggta agctgcgcag cagcggtgtg tactcctaca aagtgctcta
     4261 tttcgaaaac gaccacgaga agacgttccg tgcaccgaaa gcgtatccgg agcagtcgat
     4321 ggcggttgcg gcgacacatg acctgcctac gctacgcggt tactgggaga gcggggatct
     4381 aacgctgggc aaaaccctgg ggctgtatcc ggatgaagtg gtactgcgcg gtctgtatca
     4441 ggatcgcgaa ctggcgaagc aagggctgct ggatgcactg cataaatatg gttgtctgcc
     4501 aaaacgtgcc gggcataagg catcgttgat gtcgatgacg ccgacgctga accgtggttt
     4561 gcagcgctac attgccgaca gtaacagtgc tctgttagga ctacagccgg aagactggct
     4621 ggatatggcc gaaccggtga atattcctgg caccagttac cagtataaaa actggcgacg
     4681 caagctttcc gcaacgcttg agtcgatgtt tgccgatgat ggcgtgaaca agttgctgaa
     4741 ggatttggac agacggcgca gagctgcagc gaagaagaag tagagtgcgg ttgatgccgg
     4801 atgcgatgca tccggcagtc agtgttatca aatcaccata ttcagcagca gacagcctac
     4861 cagaccgcac accgagataa tggtttccag catcgaccag gatttgatgg tctcgccgat
     4921 agtcaggtta aagtactctt tgaacagcca gaagcccgga tcgttcacat gagagaaaat
     4981 cacactaccg gaacccaccg caataaccat cagctccggg ctaacacccg tcgttgcaat
     5041 cagcggtgcc gcgataccac ccgcagtgat tgccgcaacg gttgcggaac ccagcgcgat
     5101 acgcagtacg gcagcaatcg accaggccat cagcagcgga gaaatgttgg tttcgtgcat
     5161 catggaagca atgtatttgt ccacgccgct gtctaccagc acctgcttga acgcaccgcc
     5221 accaccgatg atcaacagca tcatcgcaat gattttgatg gaagaaacca gcgtgtcgtt
     5281 aatctgatcc attgaacgac cacggttcag accaaaggtg aacatggcaa tcagcacggc
     5341 aatcagcgta gccattaccg ggtcaccgag gaactccgct accggcagga aagcgtgacc
     5401 tttcggcagg atcatttcgg caatcgcacg catcgccatc agtactaccg gcaccagaga
     5461 ggtccagacg ctgacgccaa agctcggcat ctcttcttcg ctgaaggttt tcgcgctgta
     5521 gagaccttcc ggaattggct tatcgatacc tttcaacacg cgagcgtaaa ccggaccggc
     5581 gagaatcacg gtcgggattg ccagaatagt accgtacagc agggttttac ccatatcggc
     5641 attgaaaatg gtggcaatcg cagtcggacc cggatgcggt ggcaggaagc cgtgggtcac
     5701 agacagtgca gctgccattg gtacaccaac atacaacagt ggaatattcg cagaagccgc
     5761 gatggtaaac accagcggca gcatcagcac aaagcccact tcatagaaca gggcaaaacc
     5821 aacggtaaaa ccggtcagta ccaccgccca ctggatgtgt tttttaccaa atttggcaat
     5881 cagcgtggtg gcgatacgtt gtgcgccacc gcagtctgcc agaattttgc ccagcattgc
     5941 gccaaaaccc atgatcaggg caaggctacc gagcgtcccg ccgacaccgg ctttgatgga
     6001 gccaataact ttatccagcg gcattccttg cattaatcca acagcaagcg ccacgaggac
     6061 gagagcgatg aagccgttca ttttgaagcg gatcatcagg agcaacaaca agattacacc
     6121 gatagcaaca atgactaatg gcatgattta cctggccttt catttgttat gggtaacgtc
     6181 aattttctga cgacaaactc taattatccc aatcgggaac agagatattg cggcaccacg
     6241 actgataccc actaaaacta attattgtag tcagatgtca ggagtatgtt tggtacccat
     6301 gtgaatgata cgggtaacat ctggcgtttg agaatcacca gagcggggta aatttaaatt
     6361 atgagaggtt ggtcatatta tcgcggggaa acgaagggag ggatttgcca aagcaatgct
     6421 gtgccaacgt ctggcacatg ttcaacgtag gcccgaaatg acgctttagc gtcgcatcgg
     6481 gcaatctaca aaagagggga taacttagta gtaggagtgt tcgccgcgct ggtgttcggt
     6541 gagatcgcgc acacctttca gctccgggaa ttcgttcagc agctgcttct cgatcccttc
     6601 tttcagcgtc acatcgacca tggaacaacc gttacagccg ccgccaaatt gcagaatggc
     6661 gtaaccgtct tcggtgattt ccatcagcga aacgcgacca ccgtgaccag caagctgtgg
     6721 gttgatctgc gactgcagca tatactccac gcgctccatc agcggtgcat cgtctgccac
     6781 tttacgcatt ttggcgttcg gggctttcag cgttaactga gaacccaact ggtcggtaac
     6841 aaagtcgatc tctgcatctt ccaggtatgg tgcgcttaac tcatcaacat acgcggtcag
     6901 caggtcaaat ttcagggcag tgtcggtggc ttccacggca tccggcggac aataagaaac
     6961 accacattca gcgttaggcg tgccagggtt aatcacaaat acgcggattt gtgtcccttc
     7021 ttcctgattt gccagcagtt tggcaaagtg cgcttgtgca gcatcggaaa tacggatcat
     7081 agtaatggcc taatagttga ctattttagt tggttataat acgcccatca tcgaggctct
     7141 acaaggttcg acaaaggcac cagacctgga cagtcgccgc accattgcgt aaaagcaact
     7201 gcgcaatctc tgcgacggta cttccggtgg taacgacatc atccacaatc accatatggc
     7261 gaccttgcac gggcaattca agacgaaagg catttttcag gttgcgcttg cgcagccggg
     7321 cactgagaaa atgctgggtc gcagtggccc gtgtacgtgt gacggcttcg ctatcccatt
     7381 ggcaatgcaa ccagcgtgat aacggctgac acagcaaatc gctctgatta aatccccgac
     7441 gccagtgacg ccgctgccat aacggaacgc tgatgatgcg atccggcaat tgcaacccgg
     7501 tggtgcgacg agcgtgtaag acttccaata gtaacagacg tgacagggcg ctggcgattt
     7561 cactgcgccg ggaaaattta agctggtgga taagcggact taacggcggc gcatagtcgg
     7621 caaccgtgac cagtctttgc cagggcggcg gtttttgcag gcagcgaccg cagggaagat
     7681 gggagtgtgt ggcgggtaat ccacattgtg ggcataacgt tttatctgtg cgggtggcgc
     7741 gtgaacagac cgaacaaatc ccccaatgac ctaacgccag tggcattcgg catagccagc
     7801 ataatcccgg tactgttagc atatgttcat ccttgtaagt caaaagagaa caatagcgga
     7861 tgaataacat ctggtggcag accaaaggtc aggggaatgt tcatcttgtg ctgctgcacg
     7921 gatggggact gaatgccgaa gtgtggcgtt gcattgacga ggaacttagc tcgcatttta
     7981 cgctgcacct tgttgacctg cccggcttcg ggcgtagccg gggatttggt gcgctgtcac
     8041 ttgctgatat ggccgaagcc gtgctgcaac aggcacctga taaagccatt tggttaggct
     8101 ggagtctggg cgggctggtg gcaagccaaa ttgttttaac ccatcccgag cgtgttcagg
     8161 cgctggtcac cgtggcgtcg tcaccttgtt ttagtgctcg tgacgagtgg ccggggataa
     8221 aaccggacgt actggcggga tttcagcagc aactcagtga tgattttcag cgtacagtgg
     8281 agcggttcct ggcgttacaa accatgggga ctgaaacggc gcgccaggat gcgcgggcgt
     8341 tgaagaaaac cgttctggcg ttaccgatgc cggaggttga cgtgcttaat ggcgggctgg
     8401 aaatcctgaa aacggtcgat ctccgtcagc cgctgcaaaa cgtgtccatg ccgtttttgc
     8461 gattgtatgg ctatctcgac ggtctggtgc cgcgcaaagt ggtgccgatg ctggataaac
     8521 tttggcctca cagcgaatca tatatcttcg ccaaagcggc ccatgcgccg tttatttcgc
     8581 atccggtcga gttttgtcac ctgctggtgg cgttgaagca gagggtgtag gcaacttttg
     8641 aaatggcgag acagaaattt actggctcgc cgcagccaac tcttcttctg acaccccggt
     8701 aaagcgcatg atgtctgtaa gagggactcc ggattcaagc attattttgg ctatatgcag
     8761 ggctttggat tgttccccct cctgccgcaa tctttccgca attgtcatta aactctcctt
     8821 gtgtttcggt gaacgttcgg caacgccgtc gataaaatcg ttaaaacgta ccgcgtcgcc
     8881 ggtttgcagt atgtaattaa acagcccttt gatttgtctg tcattagcgt atccactact
     8941 taataagcag gccatttgct ctaccagccc catcagatcg cgttgacgaa tatgtttttg
     9001 aattaactcc agcagcgcca tacgtcggtg ctgcatgatt tcatcatcag gcatgacggt
     9061 gacatcaatc agcggaaatg cggaggcata aagctgcctt gccagtttgg gatcggcgaa
     9121 acaatccagc caacacagcg aatagggata ggggctttca ataccgtggt aaaacaacaa
     9181 tggcaccacc atcggcaacg ttttgtatcc agcatcaagg tgattttgca ttgcggcaat
     9241 agcgtaacgc atcatgcgaa atgcgatcag tttgtttgag gtgctttgat gttcaatcag
     9301 acaatagatg tatcctggtc cttgttccgt tttcaccgac cacagcacat cggaatagct
     9361 ttcacgcaga tcgtcatcaa taaagctgct cgactccagc ttgagggttt tcatatcaca
     9421 aagcgcgtga atgggtgccg gtaaatgaaa cgcaagaaaa tcacgagccg tgtccggttg
     9481 gcgtaaaaag agtttgaaca gcgcatcgtg tggggtggaa ctctgctttt tgctcatggc
     9541 gttccttcac ctgttgtttg aatgccgcga ctctacgcgc cactccacac ctgtgtatta
     9601 gcgagttttc atccctgcga gatgctttca aaggaactta cgccgtgctg caagagttgc
     9661 atttttatgg aaagccgcac gcaaaaaagc ccggatgcag acgcataccg ggctttccaa
     9721 aacaggcgat ggagtaaggt taacgcagcg cccaccactc gcgcagacag gcttttcctt
     9781 ccgggcagct tttacaactg ccagagaggc agccgtcagg ttcttcctga atccgcacgg
     9841 ctttgcccat actttccagt tgttgcagca tggcgttaat cattggctgt ggagtgttca
     9901 atgtctggct tatctgggcc gcttccatac ggccccgtaa cgccagcaaa tcgcgcacct
     9961 gaataagtga agccattaat ggcaatcacc ggtggtgctg gctgcgcagc aactgcttac
    10021 cgacttgcgg gttgccagca gttcgatatc cacccggcta cgcgcgcggc gcagcagacc
    10081 gataaccacg atgttaaaca ggataaccgc cagaatgcac accaggctgt aagttgggtg
    10141 ctgactgtag ctggcgacct gatagaacaa tgttgccagc gagtaagcga tattcagccc
    10201 ccacaggatg gagaagccca tccagccacg gcttgattca cgggcgatgg ctcccatcac
    10261 cgagatacat ggtacataca gcaggacgaa aatcaggtag ctgtaagctg ctgctgcgct
    10321 accgaatttc tgatccatca cgcccatcgc cccggtaccc atttcgccgt cgcctttgct
    10381 ggcttcaatg gggttcatca gtacgctaag gctgaaggtg tctttcaggc tctgccaggt
    10441 ttcatctatc gcactgaaca gctcttcacc gaggttaaac tctgccggat tgaactcttc
    10501 gtcctgaata ttttctgcgg tgtagagggt gttgagcgta cccactacca cttctttcgc
    10561 catggcacct gtaaacaggc caaccgttgc ctgccagtta tcttcatgca cgccaattgg
    10621 cttgaagacc ggggtgatca cccggctgac ggacgccagc gccgagtcgt tgatgttatc
    10681 gacgattttc ccgctcagcg agaagctgtt gaaagcgctc aggaaaatgc tgacgatgat
    10741 gatcacttta ccagcacgca gaacgaagcc tttcagacgc tgccaggtct ggataatcag
    10801 gcttttaacg tgtggtacat gatagaccgg caactccatg acaaacggcg tcgcttcacc
    10861 gcgcatgatg gtgtacttga gcatcaggcc agtcagcacc gccatcacaa tacccagcat
    10921 atacagcgag aagaccgcca gcgcaccgtt ctgcccgaag aaggcagccg cgaatactgc
    10981 gaagatagcc agacgcgcgc cgcaggacat aaacggtgcc atcatgatgg tcatcagacg
    11041 ttcacgcggt gcatcaagcg tacgtgcacc cattaccgac ggtacgttac aaccgaaacc
    11101 gacgatcagc ggcacaaagg atttccccgg caagcccagc gcctgcatca gacggtccat
    11161 cacaaacgcc gcacgcgcca tatacccgga gtcctcaagg aaggagagga acaggtacat
    11221 catgccaatc tgcggcacca gtggcagcac ggtgttaatg ccgccaccca ggccctgggc
    11281 gaggaagata gtcagccagt ccgggaagtg gagcgtgtag ccaatccatt gaataccatg
    11341 cacaaatagc gccacggagc cgacgtcaaa cagcggctgt aacgccccgc cgatgttgat
    11401 agccagcagg aacatcaggt acatcacaaa gaggaaaatc ggcagaccga ggaaacggtt
    11461 gagcacgatt ttatctaccg cagtggtgaa acggctgggt tctgccgtca gggtgttgct
    11521 taccacatca cagatggcag caatgcactg gtaacgcgca tcggcaatgt gcagcgccgg
    11581 atcgtccatc tcattacgca gacgggcgag ggcggcatcc agatgctgcg acgcttcacc
    11641 ggcgtaggcg cggctgtaga tatcgccttc cagcatttgc aggcccagcc agcgacgttg
    11701 tttcagcggg atgtcggaag gcatcacttt tgccagtgaa tctgcttcgt tgagtagcgg
    11761 ctgtgcgtaa tgcaccagtt ccacattctc gttagcttta tagcgatcaa tcgccagctt
    11821 gagcgcttca ataccgcgac cacgggttga aaccagcggg atcactgggc agccaagacg
    11881 cgccgacaga gcatcaattt caatacgaat attttgcttc tcggcaatgt cgagcatgtt
    11941 cagtgccacg atgcagggaa tgccgagttc cagcagttgt agcgtcaggt acaggttacg
    12001 ctcaaggtta gacgcatcca ccacgttaat cagcaggtcg gcgtcgccac tcaaaatgta
    12061 gtgacaggcg atttgctcat cgagcgaggt ctgcgatgag atggtggtca gagaataggt
    12121 gccgggcagg tccaccagcg tgacctgatg atcggtggtg gagaattgcc cttctttacg
    12181 ttcgacggta acgccagccc agttacctac acgctgacgt gcgccagtga gctggttaaa
    12241 taaggttgtc ttgccagaat ttggattacc aattaagcct atggttaatt ttttcattgt
    12301 tgttatcacc gtattaacag gaaaccgctt ccacttctaa taaggccaga tcttttttgc
    12361 gtaataccag gctcacacga cgggtttcga tatgaatggg gtcgccgagt ggagcgacgc
    12421 gcaccacatt aaaagaggag ccaggtaaca tgccaagaga aagcagtttt tggcgatatg
    12481 ccgggctgat ttcacgggaa aagccagtga ttttccacgc agtatctgga gtgtattgca
    12541 taggtgccta cttgtttctc attaactgga taactacctc atggggcgtt tgacagcgac
    12601 atgagaccag atgatttgcc aacgaaggag agattaaata atcaacagca caatgataat
    12661 gagaatggtt tctatcagca atacttaaaa tgtgtgcgaa tgttggtttg aataataatc
    12721 tgactcaatg ttgatgtggc tcaaggttat atcgcagcga aaatataatt cgaatagcga
    12781 attaccagga ctggctaatg tttaattaag gtttcagctg ttaatgaagt gataaatgtt
    12841 gccggaatat attgtgagtt tggctgtgga ataaaaatgc cggagatatt ctccggcact
    12901 tttatgtcag acaaggcgtt cgttgccaga tgcggcgtgg acaccttatc gcgcaggttt
    12961 aggggtcaat cagggcgttt aacgtttttt gcccattgcc gccgccagcg catccatcat
    13021 cgcgctatta ccggcaggct gcgcttcacg accgcgtggt ttggctgccg ggcggttgtt
    13081 ttgcgggcgt tcattaccgc cgccgcgacg ggcgttggtt tcgccaggct gctcgtccag
    13141 acgcatggtc agggcgatac gtttacgctg aagatccact tccagcactt tcaccttcac
    13201 aatgtcgccc gctttcacca cggtatgcgg atcttccaca aacttgttcg acaatgaaga
    13261 gatgtgaacc aggccgtcct gatgcacgcc aatatcgaca aacgcgccaa agttggtgac
    13321 gttggtgaca gcgccttcga ggatcatacc cggttgcagg tcgttcattg tctcgacgcc
    13381 atcggcaaac tgagcggttt taaattccgg acgcggatcg cgacccggtt tttccagctc
    13441 tttgatgatg tcagttactg tcggcacacc gaatttttca tcagtaaagt cagacgcttt
    13501 caggttacgc agttcgctgc tgttacccat cagatctttc agtgcctgct gtgttgctgc
    13561 cagaatgcgt tccaccaccg gataggcttc cgggtgaacg gtagaggcgt ccagcgggtt
    13621 atcaccgtgg ttaatgcgca ggaagcccgc gcactgctcg aaggctttcg gccccagacg
    13681 gctgactttc agcagttgct gacggttctt gaactggccg ttctcatcgc gccaggccac
    13741 gatgttttgc gccatcatgc gcgtcaggcc cgccacgcgg gttaatagcg gaacagaagc
    13801 ggtgttgaga tcgacgccaa cggcgtttac gcagtcttct actactgcat ccagtttgcg
    13861 ggccagttgc gtctggctga cgtcatgctg atactgacct acgccgatag atttcggatc
    13921 gattttcacc agctccgcca gcggatcctg caaacggcgg gcgatagata ccgcaccacg
    13981 cagcgaaacg tcgagatccg ggaactcctg cgctgccagc tcggaagccg agtaaaccga
    14041 cgcgccagct tcgctgacga tcactttctg cgcggtcact ttcgggaact gcttctgcac
    14101 gtcgagatag aaacgttcgg tttcgcggga agctgtaccg ttgccgatcg ccaccagttc
    14161 aacgttatgc ttttcacaca aggcagcaac ggtcatcgct gcttttgctg tttgtccggt
    14221 gtgcgggtaa atggtatcgg tcgccaccag tttgccagtg gcatcgacca ccgctacttt
    14281 taccccggta cgcagacccg gatcgaggcc catcgtggca cgcagtccgg caggggccgc
    14341 catcagcaga tcgtgcaggt tacgggcaaa gacgttgatt gcttcatctt ccgcgcgttc
    14401 gcgcacggtg cccatcagtt cggtttccag atgcatcagc accttgatgc gccaggtcca
    14461 gctcaccacg cctttgcgcc agctatccgc cggagcattg ttcaggcgca ggccgagatg
    14521 atccatgatg atttgctcgc aatagctctc ttttggcggc tcttcgaact gcggatcggc
    14581 attcagcgaa agctggagta cgccttcgtt acgcccacgg aacatcgcca gcgcgcggtg
    14641 agaaggcacc gtggacagcg gttcgtgatg atcgaaatag tcgcggaatt ttgccccttc
    14701 ctcttcttta ccgctcatca cggttgagac cagatgcgcg ttcttccaca gataatcacg
    14761 cactttcgcc agcagcgcgg catcttcggc aaaccgttcc atcaggatat agcgcgcgcc
    14821 gtccagcgcg gctttggtat ctgccacgcc tttatctgca tcaacatatt gtgcagcggc
    14881 gacttctggc gtgtgtgacg gatcgctcca cagcaggtca gccaacggct caagccctgc
    14941 ttcaatggcg atttgcccgc gggtgcggcg tttaggtttg tagggcaggt agagatcttc
    15001 gagttcggtt ttgcttagtg tggcgttgat ggccttcgcc agatcatcgg tgagtttgcc
    15061 ttgctcggaa atggatttga ggatcgcctg acgtctctct tccagctcgc gcagatagct
    15121 cagacgcgtt tccagattac gcagctgcgt gtcatccaga ccgccggtga tttccttacg
    15181 ataacgtgcg ataaacggca cggtattccc ttcgtcaagc aggcgaacgg cagcgtcaac
    15241 ctgttccggg cgcgcctgaa tttcacccgc aataatgcgg cagaacgaat cattcatcat
    15301 ggtttgagtt catcttttcg gatcaaaaat caggggatag ttatacggac tggctggcaa
    15361 aaatgccagc catcggcagg aggttaagac tcttccttac ggtttcacgt actcgatagc
    15421 attgacatac cagctcgctt ccccggcagg ggtattcacc accgccagat cgccgacttc
    15481 ttttttcagc aatgcgcggg ccatcgggga atcgatagag atgtaatctt tacggccaaa
    15541 aatttcatcg tagccgacaa tacggaaacg gtgagtcacg ccatcgtcgt tttcaatctc
    15601 cacccatgcg ccaaaaaaga ccttgccttc ctgctgaggg gagtaatcga cgattttgag
    15661 attttccatg catttagtga gatagcgcac gcgacggtcg atttcacgca gacgcttttt
    15721 attatactga tagtcagcat tttcgctgcg gtcgcccaga cttgcggccc aggtcacctt
    15781 ttttgtgacc tccgggcgtt cttcacgcca gagataatta agctcttgtt tgagtttttc
    15841 atacccttcc cgggtaacca ggggcgtttt catctcgttg attccctttg tctgtttgat
    15901 aatgcgcaca ttgggtataa cgtgatcata tcaacagaat caataatgtt tcgccgaata
    15961 aattgtatac ttaagctgct gtttaatatg ctttgtaaca atttaggctg aaattcatac
    16021 cagatttagc tggtgacgaa cgtgagcttt tttaagaata cacgcttaca aattgttgcg
    16081 aacctttggg agtacaaaca atgcaagaga actacaagat tctggtggtc gatgacgaca
    16141 tgcgcctgcg tgcgctgctg gaacgttatc tcaccgaaca aggcttccag gttcgaagcg
    16201 tcgctaatgc agaacagatg gatcgcctgc tgactcgtga atctttccat cttatggtac
    16261 tggatttaat gttacctggt gaagatggct tgtcgatttg ccgacgtctt cgtagtcaga
    16321 gcaacccgat gccgatcatt atggtgacgg cgaaagggga agaagtggac cgtatcgtag
    16381 gcctggagat tggcgctgac gactacattc caaaaccgtt taacccgcgt gaactgctgg
    16441 cccgtattcg tgcggtgctg cgtcgtcagg cgaacgaact gccaggcgca ccgtcacagg
    16501 aagaggcggt aattgctttc ggtaagttca aacttaacct cggtacgcgc gaaatgttcc
    16561 gcgaagatga gccgatgccg ctcaccagcg gtgagtttgc ggtactgaag gcactggtca
    16621 gccatccgcg tgagccgctc tcccgcgata agctgatgaa ccttgcccgt ggtcgtgaat
    16681 attccgcaat ggaacgctcc atcgacgtgc agatttcgcg tctgcgccgc atggtagaag
    16741 aagatccagc gcatccgcgt tacattcaga ccgtttgggg cctgggctac gtctttgtac
    16801 cggacggctc taaagcatga ggcgattgcg cttctcgcca cgaagttcat ttgcccgtac
    16861 gttattgctc atcgtcacct tgctgttcgc cagcctggtg acgacttatc tggtggtgct
    16921 gaacttcgcg attttgccga gcctccagca gtttaataaa gtcctcgcgt acgaagtgcg
    16981 tatgttgatg accgacaaac tgcaactgga ggacggcacg cagttggttg tgcctcccgc
    17041 tttccgtcgg gagatctacc gtgagctggg gatctctctc tactccaacg aggctgccga
    17101 agaggcaggt ctgcgttggg cgcaacacta tgaattctta agccatcaga tggcacagca
    17161 actgggcggc ccgacggaag tgcgcgttga ggtcaacaaa agttcgcctg tcgtctggct
    17221 gaaaacctgg ctgtcgccca atatctgggt acgcgtgccg ctgaccgaaa ttcatcaggg
    17281 cgatttctct ccgctgttcc gctatacgct ggcgattatg ctattggcga taggcggggc
    17341 gtggctgttt attcgtatcc agaaccgacc gttggtcgat ctcgaacacg cagccttgca
    17401 ggttggtaaa gggattattc cgccgccgct gcgtgagtat ggcgcgtccg aggtgcgttc
    17461 cgttacccgt gcctttaacc atatggcggc tggtgttaag caactggcgg atgaccgcac
    17521 gctgctgatg gcgggggtaa gtcacgactt gcgcacgccg ctgacgcgta ttcgcctggc
    17581 gactgagatg atgagcgagc aggatggcta tctggcagaa tcgatcaata aagatatcga
    17641 agagtgcaac gccatcattg agcagtttat cgactacctg cgcaccgggc aggagatgcc
    17701 gatggaaatg gcggatctta atgcagtact cggtgaggtg attgctgccg aaagtggcta
    17761 tgagcgggaa attgaaaccg cgctttaccc cggcagcatt gacgtgaaaa tgcacccgct
    17821 gtcgatcaaa cgcgcggtgg cgaatatggt ggtcaacgcc gcccgttatg gcaatggctg
    17881 gatcaaagtc agcagcggaa cggagccgaa tcgcgcctgg ttccaggtgg aagatgacgg
    17941 tccgggaatt gcgccggaac aacgtaagca cctgttccag ccgtttgtcc gcggcgacag
    18001 tgcgcgcacc attagcggca cgggattagg gctggcaatt gtgcagcgta tcgtggataa
    18061 ccataacggg atgctggagc ttggcaccag cgagcggggc gggctttcca ttcgcgcctg
    18121 gctgccagtg ccggtaacgc gggcgcaggg cacgacaaaa gaagggtaaa taaacgggag
    18181 gcgaaggtgc ctcccgtttt gctttctata agatactgga tagatattct ccagcttcaa
    18241 atcattacag tttcggacca gccgctacca gcgcggcacc cgcaggggtg tcggtgtatt
    18301 tatcgaagtt gtcgataaac agtttcgcca gggtttcggc tttttcctgc cactgttccg
    18361 gagaagcgta ggtgttacgc ggatcgagaa tcttcgtgtc tacgcccggc agttcggttg
    18421 ggatcgccag gttaaacatc ggcagagtga aggtttctgc attatccagc gaaccgttga
    18481 ggatggcgtc gataatggcg cgggtatctt taatcgagat acgtttgcca gtgccgttcc
    18541 agccagtgtt aaccagataa gcctgcgcgc ccgccgcctg catacgtttc accagcactt
    18601 ctgcgtactg agtcgggtgc agcgacagga acgccgcgcc gaagcaagcg gagaaggttg
    18661 gcgtcggttc ggtgatgcca cgctcagtac cggccagttt ggcggtgaag ccagagagga
    18721 agtgatactg ggtttgatcg gcagtcaggc gagaaaccgg cggcaacacg ccgaaagcat
    18781 cagcagtcag gaagataacc ttagtcgcgt ggcccgcttt ggaaaccggc ttaacaatgt
    18841 tatcgatgtg atagatcgga taagaaacgc gggtgttctc ggtttttgaa ccatcatcaa
    18901 agtcgatagt gccatcttca cgcacggtga cgttttccag cagcgcatcg cgacggatag
    18961 cgttgtagat ttcaggttcc gcttctttcg acagcttgat agtttttgcg tagcagccgc
    19021 cttcgaagtt aaacacgccg tcatcgtccc agccgtgctc gtcatcacca atcaggcgac
    19081 gtttcgggtc ggtggaaagg gtggttttac cggtgccgga aaggccgaag aacaccgcaa
    19141 catcgccttt ctcaccaacg ttggcggagc agtgcataga agcgatacct ttcagcggca
    19201 gcaggtagtt catcatcgag aacatccctt tcttcatttc gccgccgtac caggtgccgc
    19261 caatcagctg catgcgctcg gtcaggttaa acgccacgaa gttttcggag ttcagaccct
    19321 gttctttcca ctgcgggtta gtgcacttcg cgccgttcat aacgataaag tctggtttga
    19381 aacctgccag ctcttcatcg ctcgggcgaa taaacatgtt tttgacaaaa tgcgcctgcc
    19441 aggccacttc ggtgatgaaa cggacggaaa gacgagtatc cgggttcgca ccacagaaag
    19501 cgtcgacaac gaacagacgt ttgccggaaa gctgcctggt caccaggcct ttcagatgct
    19561 gccaggtttc cggagagaga ggtttgttgt cgttcttacc tttgcctttg tctgcccacc
    19621 agaaagtatc gcgagtggta tcgtcacgga cgatatactt atcttttggt gaacgaccgg
    19681 tgaagatccc ggtatcgacg gcaacggcac ccagattagt taacaccccg cgctcataac
    19741 ctgtcaggct cggatcgagc tcttcctgat acagcaggtc gtagcttggg ttgtaaacga
    19801 tatcatgtac gtcactgata ccataagcct cgagttcttg cggggtcaaa ccattgttaa
    19861 cgcgcatttc actgctcctt agccaatatg tattgcctga atagtaaagt ctttttgggg
    19921 gtgttaaccg cgacaaggct catagattta cgtatctgga gaaattcata tttgacaaaa
    19981 aactccgtga ttcctgtcac gaaacggttg ctattatcgc agaaatagca ttttaggtgt
    20041 ggaaaatgtt ctcaaaaggt taaatcttgg tttatatggc gggaatattg tgagtggaat
    20101 cgcattcctt aatgaaattg atgcaaattc aatcggtaaa gtatcgattc accacgctaa
    20161 ctttttttga ttattttctg aaaaaagcaa aatactactg cccggatgcg ataattaacc
    20221 caccttttga ttctggataa ccaacatgga taacgttgaa ctttcaccgg caacacgctg
    20281 ggggatgatt gctaccggat tacttcaggg gctggtttgc tacctgttga tcgcctggct
    20341 tgccggaaaa aatcacagtt ggattgttta tggcgtgcct gcgaccgttg cgttttcatc
    20401 cgttttactc ttctccgtga tctcttttaa acagaagcgc ctttggggat ggctggcgct
    20461 ggtgtttatt gccacgacag ggatgagcgg ttggctgaag tggcaaactg atggcatgaa
    20521 cccctggaga gctgaaaagg cgatttggga ttttggctgc tatctgctgt tgatggcaat
    20581 gttgttgcta ccgtggatac aacaaagcct tcgtatccgc aatggcagta gccgctatag
    20641 ctacttttac aagtcagtat ggcataacgt acttatatta ctggtgattt ttctcgccaa
    20701 tggcctgacg tgggtggtgc ttttactgtg gggtgagttg tttaaacttg ttggcatcaa
    20761 gtttttcaat acgctctttt ttgcaaccga ctggtttatg tatcttacat taggtctggt
    20821 taccgcactg gcggtgattc tcgcgcgcac acagtcacgt ttaatcgact ctattcaaaa
    20881 gttgttcacg ttaatcgcca cggggttgct gccgttagta tcattgctaa ccctgatgtt
    20941 tatcatcact ctgccgttta cgggcctgag cgcgatttct cgccacatct ccgccgccgg
    21001 gttgctgtta acgctggcct ttttgcaatt gatcttaatg gctattgtcc gcgatccgca
    21061 aaaagcgtca cttccctgga cggggccgtt gcgttgcctg attaaaaccg ctttgctggt
    21121 tgctccgctg tatgtgttcg tcgccgcctg ggcgttatgg ctgcgggtcg ctcagtacgg
    21181 ctggactgtc gaccgcttgc agggcgcgct ggcggtgctg gtgttactgg tgtggtcgct
    21241 ggggtatttt gtcagtatcg tctggcgtaa tgggcaaaat ccccttgttc ttcagggcaa
    21301 agtgaacctt gcggtttcgt tattggtgtt ggtgatactg gtgcttctta attcgccggt
    21361 gctggatagt atgcgtatta gcgtgaacag tcatatggcg cgttatcaga gcggcaaaaa
    21421 cacgccagac caggtaacta tctacatgct cgaacagagc ggtcgctatg gacgtgcggc
    21481 gcttgagtcg ctgaaaagcg atgccgggtt tatgaaagac ccgaaacgcg cgcgggatct
    21541 gctgatggcg ttagatggag agcaacatct tcaggaacag gtatcggaaa aagtattagc
    21601 cgaaaatgtg ttaattgccc ctggttctgt taaaccggat gcgacattct ggtcggcctt
    21661 aatccaggat cgctataacg tgatgacctg tattgaaaaa gacgcctgcg tcctggtcga
    21721 gcaagatctg aatagtgatg gtcaggcgga gcggatcctg tttgctttta atgatgacag
    21781 agtcattgtc tatggctttg actcagacag aaaagaatgg gacgcgcttg atatgagttt
    21841 acttccgaac gaaataacga aagaaaaatt actcacagct gcgaaggatg gaaaactggg
    21901 gacgaggccg aaagcgtggc gcgatctgac ggtagatggt gaaacgctgg aaataaactt
    21961 aagtaagtga caactctttt aacggatacg acgctgtatc tgaccaacaa taatttgcga
    22021 tttatttcac aggcctggta agcgcagcac atcaggccat tacgtcgtaa aatccggcag
    22081 agccctctct gccggacata ctcattaatg aacttgcgga tctgccggag acgcgttgtt
    22141 gcggatttca gcaatatcca tcgcattgaa cagatagtgg ttaccgcagt aatcacaatg
    22201 catgtcaatt tcgccatctt ccgtcaggat gctatcaact tcttcatcag gcagcgtttt
    22261 cagcgcatcg gcgcaacgtt cacgcgagca ggtgcatttg aactccacat cctgcggatc
    22321 gtaaaccgtc acctcttctt cgtgatacaa acgccacaac acttcgtttg ccggtaaggt
    22381 cagcagttct tcggttttga tggtttcggt tagcgtcgcc aggtggtcaa agtcgtcctg
    22441 ctgggcattt tgcgcaggca ttacctgcaa caacataccg cctgcagccg gtttgccgtc
    22501 tacgtcgccg gtgcgaataa acaggcgcgt cggcagctgt tcagaacgca taaagtaatc
    22561 ttccaggcag gccgccaggg tatcaccttc cagaccaacc acgccctgat agcgttcgcc
    22621 ttcgctcggg gtaatggtga tcaccacgta accattgccg accagcgttt tcaggtcggc
    22681 attttctgga atttcgccct gcacgcgcgc cacaccgcgc atttgctggt tattgttacc
    22741 gttaataacc gccagattca tcggaccgtc gccttgcagc tgtacggtga tatcaccatc
    22801 aaacttcagc gtagcggtta acaggctggt cgcaaccagc agttctgcca gcacgttttt
    22861 aacgggctgc ggataatcgt ggttctcaag gatctgttgc agggtttccg aaacggttac
    22921 cagttcgccg cgaacggcaa aattttcaaa cagatagcga tgtaattggt catgttgcgg
    22981 cataatcatc tctcttgcag gtgacagtta ttcactgtcg ccgtgtttaa atcgtaacag
    23041 gtcgcggcgc tcttttttgt ccgggcgtcg gtccgggtgc ggcatggtta aggcattaag
    23101 tttacgtgcc atcgccattt tttcgcgttt ctctacactt tccgcagtct cttcatacag
    23161 caaggctgcc tcgctggcgg ggcgacgctg ttcagtaatc gcctttacaa tcaccgtgcg
    23221 ttcgtcattt ccctggcgca gagtgagcgt ggcattcagc tcgacgattt tgctcggctt
    23281 gctgcgctgc ccgttgtaat gcaccttacc gccttcaatc atttcacggg ccagcgcgcg
    23341 ggttttataa aaacgggcag cccatagcca tttatccagt cgaacctcaa cagcaggttt
    23401 ctctttcatg gcgtctcctt cacattagcg aggggatcag gcggcggtag tcattcagtg
    23461 acggatggcg ttgatactgt ttctcggcaa tcccggaatc aggattagtc acgccgaggc
    23521 agtaacgaat accaaattgc gcggcagcat cgagaatcgc ttcgctgtca tcaataaaca
    23581 gcgttctttc agctttcaga cccgtagctt cggccaccgc atgccataac cgctgatcct
    23641 ctttcggata accaaatgtg tgggtggaaa gtaataaatc aaggtgtgcg tccagaccgg
    23701 tatgctcaag ttttaccgcc aggttgtgcg gatgcgcatt ggtgagcaaa attcgctgct
    23761 taccgctggc tttcagtgcc tcaagaaacg gaatggtatc ttcacgcagt acggcacgcg
    23821 gtcccatctc ggtggtcatc gcacagatat ccagacccag ttgctcactc cagtaatcaa
    23881 gacagtacca gtttagcgta tgctgtacgt cgtgatattg ctggcgcata tattccatcg
    23941 cttcctgtgg cgtaaccccg tttttggcgc cccatgtttc aggcaccagc ttttgccaga
    24001 aatagttatc gaaggcgagg tcgagcaacg tgccgtccat atccagcaga acggtatcta
    24061 cgtcctgcca ggcaatgttg atatgcatga gggaaatctc cagagtgaag caatttgcgc
    24121 gacagggtag cataacctgc cgcgcaaacg tgttattcga taaggctttc tgaaggggtg
    24181 atcagttgcg ggttcaggca gctttcataa taggcctgaa tctccatcat gcgagtgcga
    24241 tgacgctggt aacgacgcca ggcctgtacg ccattgtaaa tggcgctacc cagcatcgtc
    24301 agtagcagca atgtggtgct gagatagcgc cacagtccgg aacgatccgg gatcggatga
    24361 aggccaatat gctgcgtacc attggcgtca gtgaagattt ttgtgacgat accttcggcg
    24421 ttaaacggcg tatgcatcag catttgtgcc agtttctgga aagcgttcca ctgttcttgc
    24481 ggcgggtagt cgtaaagtga tgccgaaggc cagggctgat caacaaaatc gctgccttcg
    24541 tcgctgacaa tcaggaatcc gccgggggcc ggactgttta gtgattgtgc cgctcgcgcc
    24601 gtttcatgcg tgataaacgg cgcggtggag gttgccacca ggttatccag cgattctgca
    24661 ctcaccgggc gtaataacac attcacgcca tcgagcttcc cggcgttggc gcgttttacc
    24721 agcgcgtccc agtctttact gttgccgaga ttgaccagcg catttttcag tcgcacacag
    24781 tcatctttgg cagaacataa atccgctgtc ttcagtacaa tgtcgccaaa atcatcaagc
    24841 aataccatgc cggatttttg aatcgctgaa cgtaatgagg cactgacgcg agattcatct
    24901 tccggtttag ggtgcagctg gcgattaact gcttcagtca atgccgtcgc tttgttgacc
    24961 agttcagatt ctggtaatgg taatgagcgg gcgtcattcc agatgatctg cgagcagtca
    25021 aacggtaaaa aaggtgaatt ggttttcgcg ctccaggttc cggaagtgcg aatattacac
    25081 attcccgtac cgctaatacg caatgtatcg cctacccgca cgccagcgtc agccagttgt
    25141 tttacgctgg tggcttcaat ggtctgcgcg cctttcatcc atgagagagt gaatttcagc
    25201 ggcatatcca gcgggaccca gaataacagc ataaacagca ccagcagcga gcccgcagcg
    25261 ataatcgtac tgcgcagcca gtgctgtaac ggaaagtttt ttacttcatc atgcagcgaa
    25321 agatatcgcc cctgacgcac tacgtggcgg tcgagataga tatcaatatc ggtttgttga
    25381 ccgagatcct gagcaatgta tggctgccag tgtgcgggat agaccaggtc gataataccg
    25441 agtgaaatat tgttgatctg ttcctgatcg ttttcgccaa acaatcccca acgacggggt
    25501 gtaccgcgca gacaatgaat ttctcgcagg gaggattttg ccgggggcgc gaatagcccc
    25561 cacagacctg cgcccagcag cagtaatgcg ccgcctgcca gccacggaac aaatacatcc
    25621 ggggtaatca ggcagaaaaa gaacatcagg aaggaggcga cgatcagcaa cgcttcacgc
    25681 agcccgcgcg gacgactcag cgcatattct tcatgcgttt ctttgcgaat attgagtagc
    25741 tcgatttgct cactctcttc cccgcgaatc gacgcctgcg tggacggaac aggttgcaag
    25801 gcatagccgc gcgtttcctg catgtactcc tgcagcgtat gaccgttgag tgaaataacc
    25861 agcggcagcg aatcggtata aatcagttca acggtattct catcgttgat gtattgttcc
    25921 cagaaagggg gcaggtggac ttctaccgaa tcgaggtagt aacgccattt attcggatcg
    25981 tcggtagaga tgccgtaacg cgtgatagcg tgtgtcagca tcatgacgtt gttgctttcg
    26041 gcattcagcg ccagagagat cggtgccgcg ctggctcccg ttgggccagg cacctgtaat
    26101 acctgcgtca ggctctcaag ataattttca acggcgctac gttcttcagg tgtgagttta
    26161 cgcgtttgcg catccgcgaa ggcgttggtc cagggcagct gacgccgtct ggatcgcact
    26221 tttatcagcc atcccgcaag tagtgagcag gccagcaaag cagctaaaaa aatcacaatg
    26281 gtgctcatgc tttccccatc ttacttagtc tgtcaggcgt ggtcagtcgc accagacaca
    26341 atacatgatt ctgcggttgg ctatcggcaa gcgtggagtt taacttgagc atcattaagc
    26401 acatcacagt aaccgctgat catagcaaac cctctgaagg cggaatatca gcaaattcct
    26461 ggagttattt caacctattg acttttaatt tgatttgagg acgattattt attaagttac
    26521 ataaatgtaa ataacatgca attcacattg ccgaaagcgt gtccgatatc gcacaataac
    26581 cgctgatttc acagcataga tcccacgatg agcaaatcat tacaaaaacc caccattctg
    26641 aatgttgaaa ctgtagcccg ttcccgactg tttaccgtcg agagcgtgga tctggagttc
    26701 agcaatggcg tgcggcgtgt ttatgaacga atgcgtccaa ccaaccggga agcagtgatg
    26761 attgtgccga ttgtggacga tcacctgatc ctgatccgcg aatacgcagt gggaactgaa
    26821 tcctacgaat taggtttttc gaaaggatta attgatccgg gtgaaagcgt ctacgaagcc
    26881 gctaaccgcg agctaaaaga agaggttgga tttggtgcga acgatctgac ttttttgaag
    26941 aagctcagca tggcaccgtc ttacttttcc agcaaaatga atatcgtggt cgcgcaagat
    27001 ctctacccgg aatcgctgga aggcgatgag ccagagccgc taccacaggt gcgctggccg
    27061 ctggcgcata tgatggattt gctggaagac cctgacttca acgaagcgcg taatgtcagc
    27121 gcgctgttcc tcgtgcgcga atggttgaaa gggcaggggc gagtgtaaat gcgtgaagtt
    27181 tgcctgatgc acaacgctta tcaggcttat gggtttcctg caatatattg attttttcac
    27241 gattttgtta gccggataac gcgttcaccc gcatccggca taaacaaatg cactttgtca
    27301 gccaactgaa aaggcgccga agcgcctttt taatcagaac aattcctgtg cctcaccatt
    27361 atcgataatg gtcgttccca cctcgtgcac cgcctgttgt gtcggctgcg taccttcgat
    27421 gaaatactct tcgcggctgt tgccaccatt agctaactgc ccggtgctgc gatcgatatt
    27481 caccgtcaca atacccggag gcggcgtcag cggctgctcc ggcacacctt caagaacggc
    27541 tttcatataa gcgtcccatg caggctgggc actcttggca ccgccttcgt aacctgagat
    27601 ctgatcttta atcgctccgg aagccgttgt atgaccgaga ttacgacggt gatcatcaaa
    27661 gccaatccag accgaggtca caacgcccgg accgtaaccc gagaaccacg catctttcga
    27721 actgttagtg gtaccggttt tcccgccgat atcgcgacgc tgcaaatcac gacctgcacg
    27781 ccagccggta ccctgccagc ctggttcacc aaagatattg gtgttcaaag cactcttaat
    27841 caggaatgcc agcggagtgt tgatgacgtg cggtgcgtac tcctgcgcgc cagtcttcgc
    27901 cactaacgcc tgatttgcct gctccagctg cggcattggt acagaaacat tctgctgctc
    27961 gcgggagata gcgacatctt caacatcgtt attttccagc acgttcgatt tctgcgtatc
    28021 accgtaaatc accggaatat cgcattccgg gcaggctact ttcggtttcg cttcgaaaat
    28081 cacgccgccc tgatcgtttt caattttgct gataaaccac gggtccacca ggaagccacc
    28141 gttcgccatg accgcgtagc cgcgcgccac ctgcattggg gtgaaggacg ctgaacccag
    28201 cgccagcgat tcggtgtgga caatgttttg cgccgggaag ccgaagcgtt gcagatattc
    28261 tgcagcgtag tcgacgccca tcgcccgcat tgcgcgtacc atcaccacgt ttttcgactg
    28321 acccagcccc tgacgtaagc gaattggacc agcatactgc ggtggtgagt tcttcggctg
    28381 ccagtcagaa ccggcacctg catcccagcg agaaattggc acatcgttca acatacttgc
    28441 cagcgtcaga cctttatcca tcgccgcggt gtagaggaac ggtttgatgt tggaacccac
    28501 ctgacgcagt gcctgggtgg cgcggttaaa cttgctctga ttgaaatcaa agccaccgac
    28561 cagcgccata acggcaccgt tttgcggatt gatcgacacc agcgccgagt tcacttccgg
    28621 cacttgtgcc agccaccatg catcgccaac ctgacgaacc cagatttgct gacccgtttg
    28681 cagaacatcg gtcactttac gcggcgtcgg tccttgctga gtatccgaac ggtaaggacg
    28741 cgcccagcga acgccttcca tactcaatgc gacggtcgac ccgtccgcca gcatcgccgt
    28801 cgcttgctga ggattggcgc tggtgactgc ggcaggcagc agcggaccat aggttggcag
    28861 cgccttcagc gtatcggtaa tcttgttgtt atcccacgcc gactcgccca ctttccacag
    28921 cacatttgcc gggccgcgat agccgtggcg catgtcgtag tccagcacgt tattacgtac
    28981 cgcctgctgt gcggcctgct gcactttgcg ggtgatggtg gtgtaaatgc gataaccgtc
    29041 ttcataggca ctttcgccat aacggttata catctcctgg cgcaccattt cgctcaggta
    29101 cggcgcagag aaagcaatct ccggcgcgtg atagttagcg ttaatcgcct cagtgcgtgt
    29161 ctgatcgaac tgttgttggg tgatataccc ttcatccagc atccgcgaca gtacgacgtt
    29221 acgccgcgcg acggcacgat ccattgagta gagcggatta aaggtggaag gcgctttcgg
    29281 cagcccggcg atcacagcca tttcgttcag cgtcagttgg tcgaccgttt taccgaaata
    29341 gacttgtgcc gcagcaccga caccataggc gcggtaaccg aggtaaatct tgttcagata
    29401 aagctcgagg atctcgtctt tcgtcagcaa ctgttcaatg cgaatcgcga ggaagacttc
    29461 cttaatctta cgcatcagcg tgcgttctgg actgaggaag aaatttctcg ccagctgctg
    29521 ggtaatggta ctcgcccctt gtgacgcgtg gccggagaac agcgccacac ttgccgcacg
    29581 gaagatcccc accgggtcaa cgccgtgatg ctcgtagaag cggctgtctt ccgtcgcgat
    29641 aaaggctttc accatctccg gtgggatttg atccaacgta accggaatac gacgtttctc
    29701 accgtattga gcaatcagct cgccatcggc gctgtaaatc tgcatcggaa tttgcaggcg
    29761 aacatctttt aatgtcgcca catccggcag ttgtggctcg atgtagcggt ataggccata
    29821 aatcgagcct gctcccagca gaatgcaaca gactgcaagg atcaaaaaat actttacgaa
    29881 cttcactgga aatttcccat ttagtttcat ttgggcagtt tataaacaaa cgcgcggtag
    29941 tataaaggca agccagacgc attgatatac ccgtcagagt gacgggtgat aaggagagca
    30001 tcacaatggc atttaagatc tggcaaattg gtttgcattt acaacagcaa gaagcggtag
    30061 cggttgcgat cgtgcgggga gcaaaagaat gctttttgca acgctggtgg cggttgccgc
    30121 tggagaacga cattatcaaa gatgggcgga ttgttgatgc gcagcagctg gctaaaacgt
    30181 tgttaccgtg gagtcgcgaa ctgccgcagc gtcatcacat tatgttggcg tttcccgcca
    30241 gtcgcacatt acagcggtca tttccgcgcc cgtcgatgtc ccttggtgag cgggagcaaa
    30301 cggcctggct gtcagggacg atggcccgcg agctggatat ggatccggac tccctgcgtt
    30361 tcgattacag cgaagactca ctcagtcccg cctataacgt gactgccgcg caaagcaaag
    30421 agctggcaac gctgcttact ctggcagaaa ggttgcgtgt tcatgttagt gcgatcaccc
    30481 cggatgccag tgcattacag cgattcctgc cttttttacc ttctcatcag caatgtctgg
    30541 cctggcgtga taacgaacag tggctgtggg cgacgcgcta tagctggggg cgcaaactgg
    30601 cggtggggat gactagcgcg aaggagctgg cggcagcgtt atccgttgat cccgacagcg
    30661 tcgcgatatg tggcgaaggc ggatttgacc cctgggaggc cgtttctgtt cgtcagccgc
    30721 cgctaccgcc gcctggtgga gactttgcca tcgcgctggg gctggcgctt gggaaggcgt
    30781 actgatgaac ccgccaatta attttttgcc ctggcgacag caacgccgga ccgcttttct
    30841 gcgtttctgg ttgctgatgt tcgttgcgcc tctgctgctg gccgtcggga taacgctaat
    30901 actgcgtctg acaggcagcg ccgaagcgcg catagacgca gttttgcttc aggcggaaca
    30961 acaactcgcc cgcagcttac agataacgaa gccacgtttg ctggagcaac agcagttacg
    31021 cgaacagcgt tcgcaacggc agcgccagcg acaatttacc cgcgactggc aatctgcgct
    31081 ggaagcactg gcggcgcttt tacccgagca cgcctggctg acaacgataa gctggcagca
    31141 gggaacgctg gagatcaagg ggctgacaac aagcattacc gcgttaaacg cactagaaac
    31201 gtcactccgc caggatgcgt cttttcatct caatcagcgg ggagccacgc agcaggatgc
    31261 gcagggacgc tggcaatttg agtatcagtt aacaaggaag gttagcgatg aacatgttct
    31321 ttgactggtg gttcgccaca tcaccccgcc tccgccagtt ttgctgggca ttctggttgc
    31381 tgatgttagt tacgctcatt tttctgtcat cgacacacca tgaagagcgc gacgcattaa
    31441 ttcgactacg ggcaagtcat caccagcagt gggccacact gtatcgcctg gttgacacca
    31501 ctcccttcag cgaagaaaaa acgctgccct tttcgccact ggattttcag ttgcccggcg
    31561 cgcaactggt ttcctggcat ccatccgcgc agggaggtga gttggcgttg aaaacgctgt
    31621 gggaagcagt gccgtcggca tttacacggc tggcagagcg caacgtcagc gtgagccgtt
    31681 tttcgttaag cgtggaaggt gatgatcttt tgttcacgct acaactggag acaccgcatg
    31741 agggttaaac gctggttgtt ggcaggtatt gcattgtgcc ttttaaccgg tatgcgtgac
    31801 ccttttaaac cgccggaaga tctatgccgg attagcgaac ttagccagtg gcgctatcag
    31861 gggatggtag ggcgaggcga gcacatcatc ggtgtaataa aagacgggca aaagaaatgg
    31921 cgacgggtgc agcaaaacga tgtgctggaa aacggctgga caattttaca gctgacgcca
    31981 gacgcactaa cgctgggaac cgggacaaac tgcgaaccgc cacaatggtt gtggcaacgg
    32041 caaggagata caaatgaagc aatggatagc cgcactactg ttgatgctga tacccggcgt
    32101 acaggcggca aagccgcaaa aagtgacgct gatggtggat gacgttccgg tagctcaggt
    32161 gttgcaggcg ctggctgaac aggagaagtt gaacctggtc gtgtcgccag acgtcagcgg
    32221 tacggtgtcg ttacatctaa cagatgttcc ctggaagcag gcactacaaa ctgtagtgaa
    32281 aagcgccgga ctgataacgc ggcaggaaga caacattctc tcagtgcatt ccattgcctg
    32341 gcagaataac aatatcgccc gccaggaggc ggagcaggcg cgggcgcagg caaatctgcc
    32401 gctggaaaat cgcagtataa ccctgcaata cgccgacgcg ggagaactgg cgaaagcggg
    32461 ggagaagcta ctgagtgcca aagggagtat gaccgtcgat aaacgcacca atcgcctttt
    32521 gctacgagat aacaaaacgg cgttaagcgc gcttgaacag tgggtagcgc aaatggatct
    32581 gccggtcggg caggttgagc tgtcggcgca tattgtcacc attaatgaaa aaagtttgcg
    32641 tgagttaggc gtgaaatgga cgctggccga tgcgcaacac gctggtggcg ttgggcaagt
    32701 caccacgctt ggtagcgacc tctccgtagc gacggcgaca acgcatgtcg gttttaacat
    32761 tgggcgcatc aacggacgct tgctggatct tgagctttct gcgctcgaac aaaaacagca
    32821 gctggatatt atcgccagtc cgcgtctgct ggcctcacat cttcagcctg ccagcattaa
    32881 acaggggagc gaaattccat atcaggtttc cagcggggaa agtggcgcga cgtcggtgga
    32941 atttaaagag gccgtcctgg ggatggaggt cacgcccacg gtgttacaaa aaggtcgcat
    33001 ccggctgaaa ttacacatca gccagaacgt tccggggcag gtgctacagc aggccgatgg
    33061 cgaagtgctg gcgattgata agcaggagat cgaaacgcag gtcgaggtca aaagcggaga
    33121 aacgttggcg ctgggcggca tttttacccg taaaaataaa tcgggtcagg atagcgtacc
    33181 gttgcttggc gacattccct ggttcgggca attatttcgt catgacggaa aagaagatga
    33241 acgacgcgag ttagtggtgt ttatcacgcc acgactggtt tccagtgagt aaacagccgt
    33301 aaaagcggta atgtttttat gctgaacgtg tttcatctat ttgacgcgcg caggtattta
    33361 gcatacaagg agtaccgatt tgagagttgg tgctcttcgc tgcctgcgtt ccatgatgat
    33421 gatttatcat tcaggcggca ttttgctgtc ttttttacgc taatcttacc cggtgattta
    33481 tcgccagagc ggtggtagca aggcagcgcg cttgcagcga ccagatatgc agagggatgg
    33541 gtgatttatt cagttgccaa acccgctgga gtattgagat aattttcagt ctgactctcg
    33601 caatatctta tgaggtttca gttcatgtcc tgcggcgctc tctgagcgaa gcgggtttat
    33661 cattaacgaa tagtcttagt agtaccgaaa aaatggcaga gaaacgcaat atctttctgg
    33721 ttgggcctat gggtgccgga aaaagcacta ttgggcgcca gttagctcaa caactcaata
    33781 tggaatttta cgattccgat caagagattg agaaacgaac cggagctgat gtgggctggg
    33841 ttttcgattt agaaggcgaa gaaggcttcc gcgatcgcga agaaaaggtc atcaatgagt
    33901 tgaccgagaa acagggtatt gtgctggcta ctggcggcgg ctctgtgaaa tcccgtgaaa
    33961 cgcgtaaccg tctttccgct cgtggcgttg tcgtttatct tgaaacgacc atcgaaaagc
    34021 aacttgcacg cacgcagcgt gataaaaaac gcccgttgct gcacgttgaa acaccgccgc
    34081 gtgaagttct ggaagcgttg gccaatgaac gcaatccgct gtatgaagag attgccgacg
    34141 tgaccattcg tactgatgat caaagcgcta aagtggttgc aaaccagatt attcacatgc
    34201 tggaaagcaa ctaattctgg ctttatatac actcgtctgc gggtacagta attaaggtgg
    34261 atgtcgcgtt atggagagga ttgtcgttac tctcggggaa cgtagttacc caattaccat
    34321 cgcatctggt ttgtttaatg aaccagcttc attcttaccg ctgaaatcgg gcgagcaggt
    34381 catgttggtc accaacgaaa ccctggctcc tctgtatctc gataaggtcc gcggcgtact
    34441 tgaacaggcg ggtgttaacg tcgatagcgt tatcctccct gacggcgagc agtataaaag
    34501 cctggctgta ctcgataccg tctttacggc gttgttacaa aagccgcatg gtcgcgatac
    34561 tacgctggtg gcgcttggcg gcggcgtagt gggcgatctg accggcttcg cggcggcgag
    34621 ttatcagcgc ggtgttcgtt tcattcaagt cccgacgacg ttactgtcgc aggtcgattc
    34681 ctccgttggc ggcaaaactg cggtcaacca tcccctcggt aaaaacatga ttggcgcgtt
    34741 ctaccagcct gcttcagtgg tggtggatct cgactgtctg aaaacgcttc ccccgcgtga
    34801 gttagcgtcg gggctggcag aagtcatcaa atacggcatt attcttgacg gtgcgttttt
    34861 taactggctg gaagagaatc tggatgcgtt gttgcgtctg gacggtccgg caatggcgta
    34921 ctgtattcgc cgttgttgtg aactgaaggc agaagttgtc gccgccgacg agcgcgaaac
    34981 cgggttacgt gctttactga atctgggaca cacctttggt catgccattg aagctgaaat
    35041 ggggtatggc aattggttac atggtgaagc ggtcgctgcg ggtatggtga tggcggcgcg
    35101 gacgtcggaa cgtctcgggc agtttagttc tgccgaaacg cagcgtatta taaccctgct
    35161 caagcgggct gggttaccgg tcaatgggcc gcgcgaaatg tccgcgcagg cgtatttacc
    35221 gcatatgctg cgtgacaaga aagtccttgc gggagagata cgcttaattc ttccgttggc
    35281 aattggtaag agtgaagttc gcagcggcgt ttcgcacgag cttgttctta acgccattgc
    35341 cgatagtcaa tcagcgtaac aacaagaaag gtcaggccgc ttatcaagcg gtctattagc
    35401 ttcaggttaa ttgcaatgtg gtaagcatta accttttagt ggggtgttaa atggatgaat
    35461 tcaaaccaga agacgagctg aaacccgatc ccagcgatcg tcgtactggt cgttctcgtc
    35521 aatcttctga acgttctgag cgtactgaac gtggcgaacc gcagatcaat tttgatgata
    35581 ttgaacttga tgacactgac gatcgccgtc cgactcgtgc gcaaaaagag cgcaatgagg
    35641 aaccggaaat cgaagaagaa attgacgaat ccgaagatga aaccgtggat gaagagcgcg
    35701 tagagcgtcg tccgcgtaag cgcaaaaaag cagccagtaa acccgcttct cgtcagtata
    35761 tgatgatggg cgtcggcatt ctggttctac tgctgttgat catcggtatc ggttctgcgc
    35821 taaaagcccc ctcgacctct tccagcgatc aaaccgcgtc tggcgagaag agtattgatc
    35881 ttgcaggcaa tgcgaccgat caggcgaatg gggtgcagcc agcgccggga accacgtctg
    35941 cggaaaatac tcagcaggat gtttctctgc caccgatctc ttctacgccg actcaagggc
    36001 aaaccccggc ggcaacggat ggtcaacaac gtgttgaagt gcagggtgac ctgaacaatg
    36061 cgctgaccca gccacaaaat cagcaacagt tgaacaatgt ggcggtcaat tctacattgc
    36121 cgactgaacc agcaactgtc gcgccagtac gcaatggcaa tgcatcgcgt gacacggcga
    36181 aaacgcaaac tgctgaacgt ccggccacta cgcgtccagc tcgtcagcag gcggtgattg
    36241 aaccgaaaaa accgcaagca accgtgaaaa cggaaccgaa gccggtagca cagacgccga
    36301 agcgtactga accagctgct cctgtggcga gcacgaaggc accggctgcg acttctgcgc
    36361 cagcaccaaa agagacggcg actacggctc cagtacagac ggcatccccg gcgcaaacca
    36421 cggcaacacc agccgctgga gggaagaccg caggtaatgt tggttcgttg aaatcggcac
    36481 cgtccagcca ttacactctg cagctgagca gttcctctaa ctacgacaac ctgaacggtt
    36541 gggcgaagaa agagaatctg aaaaactacg ttgtctatga aacgacgcgt aatggtcagc
    36601 cgtggtatgt cctggtttct ggcgtgtacg cttcgaaaga agaggcgaaa aaagcggtat
    36661 ctacattgcc agcagatgtt caggccaaaa acccgtgggc gaaaccgctg cgtcaggtac
    36721 aggccgatct gaagtaatca aggttatctc ccgcaatggg ttatcgttgc gggagttgcc
    36781 tgaagcgctg gatgctgtcg gagctttctc cacagccgga gaaggtgtaa ttagttagtc
    36841 agcatgaaga aaaatcgcgc ttttttgaag tgggcagggg gcaagtatcc cctgcttgat
    36901 gatattaaac ggcatttgcc caagggcgaa tgtctggttg agccttttgt aggtgccggg
    36961 tcggtgtttc tcaacaccga cttttctcgt tatatccttg ccgatatcaa tagcgacctg
    37021 atcagtctct ataacattgt gaagatgcgt actgatgagt acgtacaggc cgcacgcgag
    37081 ctgtttgttc ccgaaacaaa ttgcgccgag gtctactatc agttccgcga agagttcaac
    37141 aaaagccagg atccgttccg tcgggcggta ctgtttttat atttgaaccg ctacggttac
    37201 aacggcctgt gtcgttacaa tctgcgcggt gagtttaacg tgccgtttgg ccgctacaaa
    37261 aaaccctatt tcccggaagc agagttgtat cacttcgctg aaaaagcgca gaatgccttt
    37321 ttctattgtg agtcttacgc cgatagcatg gcgcgcgcag atgatgcatc cgtcgtctat
    37381 tgcgatccgc cttatgcacc gctgtctgcg accgccaact ttacggcgta tcacacaaac
    37441 agttttacgc ttgaacaaca agcgcatctg gcggagatcg ccgaaggtct ggttgagcgc
    37501 catattccag tgctgatctc caatcacgat acgatgttaa cgcgtgagtg gtatcagcgc
    37561 gcaaaattgc atgtcgtcaa agttcgacgc agtataagca gcaacggcgg cacacgtaaa
    37621 aaggtggacg aactgctggc tttgtacaaa ccaggagtcg tttcacccgc gaaaaaataa
    37681 ttctcaagga gaagcggatg aaacagtatt tgattgcccc ctcaattctg tcggctgatt
    37741 ttgcccgcct gggtgaagat accgcaaaag ccctggcagc tggcgctgat gtcgtgcatt
    37801 ttgacgtcat ggataaccac tatgttccca atctgacgat tgggccaatg gtgctgaaat
    37861 ccttgcgtaa ctacggcatt accgccccta tcgacgtaca cctgatggtg aaacctgtcg
    37921 atcgcattgt gcctgatttc gctgccgctg gtgccagcat cattaccttt catccagaag
    37981 cctccgagca tgttgaccgc acgctgcaac tgattaaaga aaatggctgt aaagcgggac
    38041 tggtatttaa cccggcgaca ccgctgagct atctggatta cgtgatggat aagctggatg
    38101 tgatcctgct gatgtccgtc aatcctggtt tcggcggtca gtcgttcatt cctcaaacac
    38161 tggataaact gcgcgaagta cgtcgtcgta tcgacgagtc tggctttgac attcgtctgg
    38221 aagtagacgg tggcgtgaag gtgaacaaca ttggcgaaat cgctgcggcg ggcgcggata
    38281 tgttcgtcgc cggttcggca atcttcgacc agccagacta caaaaaagtc attgatgaaa
    38341 tgcgcagtga actggcaaag gtaagtcatg aataagtttg aagatattcg cggcgtcgct
    38401 tttgatcttg atggtacgct ggtcgacagt gctcccggtc ttgctgctgc ggtagatatg
    38461 gcgctgtatg cgctggagtt gcccgtcgca ggtgaagaac gcgttattac ctggattggt
    38521 aacggcgcag atgttctgat ggagcgcgca ttgacctggg cgcgtcagga acgtgcgact
    38581 cagcgtaaaa caatgggtaa accgcccgtt gatgacgaca ttccggcaga agaacaggta
    38641 cgtattctgc gtaaactgtt cgatcgctac tatggcgagg ttgccgaaga ggggacgttt
    38701 ttgttcccgc acgttgccga tacgttgggc gcgttgcagg ctaaaggcct gccgctaggc
    38761 ctggtcacca acaaaccgac gccgttcgtc gcgccgctgc tcgaagcctt agatattgcc
    38821 aaatacttta gcgtggttat cggcggcgat gatgtgcaaa acaaaaaacc gcatccggac
    38881 ccgctgttac tggtggctga gcggatggga attgccccac aacagatgct gtttgtcggc
    38941 gactcacgca atgatattca ggcggcaaaa gcggcaggtt gcccatcagt tggcttaacc
    39001 tacggatata actacggcga ggctatcgat ctcagccagc ctgatgtaat ttatcagtct
    39061 ataaatgacc ttctgcccgc attagggctt ccgcatagcg aaaatcagga atcgaaaaat
    39121 gactaagccc atcgttttta gtggcgcaca gccctcaggt gaattgacca ttggtaacta
    39181 catgggtgcg ctgcgtcagt gggtaaacat gcaggatgac taccattgca tttactgtat
    39241 cgttgaccaa cacgcgatca ccgtgcgcca ggatgcacag aagctgcgta aagcgacgtt
    39301 ggatacgctg gccttgtatc tggcttgtgg tatcgatcct gagaaaagca ccatttttgt
    39361 tcagtcccac gtaccagaac atgcgcagtt aggctgggca ctgaactgct atacctactt
    39421 cggcgaactg agccgcatga cgcagtttaa agataaatct gcgcgttatg ccgagaacat
    39481 caacgctggt ctgtttgact atccggtgct gatggctgcg gacatcctgc tgtatcaaac
    39541 taatctggta ccggtgggtg aagaccagaa acagcacctg gaactgagtc gcgatatcgc
    39601 ccagcgtttc aacgcgctgt atggcgagat ctttaaggtg ccggagccgt ttattccgaa
    39661 atctggcgcg cgcgtaatgt cgctgctgga gccgaccaag aagatgtcca agtctgacga
    39721 taaccgcaat aacgttatcg gcctgctgga agatccgaaa tcggtagtga agaaaatcaa
    39781 acgcgcggtc actgactccg acgagccgcc ggtagttcgc tacgatgtgc agaacaaagc
    39841 gggcgtttcc aacctgctgg atatcctttc tgcggtaacg ggccagagca ttccagaact
    39901 ggaaaaacag ttcgaaggca agatgtatgg tcatctgaaa ggtgaagtgg ctgatgccgt
    39961 ttccggtatg ctgactgaat tgcaggaacg ctatcaccgt ttccgcaacg atgaagcctt
    40021 cctgcaacag gtgatgaaag acggcgcaga aaaagccagt gtacacgctt cccgtacgct
    40081 gaaagcggtg tacgaagcga ttggttttgt ggcgaagccg taatatcaaa acgctcggac
    40141 ggtcccctcg cccctttggg gagagggtta gggtgagggg agcaggcaag cgccagtggg
    40201 aattaccctt tgctcgcatt gtcgaataaa tcacttcgtc atcactctga aaccggggaa
    40261 acccggtttt tttatcctca tttgcaataa tccgaaaaaa tgtgaagcgc ctcgccgttt
    40321 ccacatctca cagttgccac ttatcttcat tttaatgaag atataaacat tcattttatt
    40381 gaaaatatat tatgcgtcga acgtttatca aaaaagaagg cgtcgttatc acgacgctgg
    40441 cccgttattt gttgggtgaa aaatgcggta ataggttgaa aaccatagat gagctggcaa
    40501 ctgaatgccg ttcatccgtt ggcctgacgc aggccgcgtt gaaaacgctg gaatcaagcg
    40561 gagcgatacg gattgaacgc cgtgggcgca atggcagtta tctggtcgag atggataaca
    40621 aagcattgct gagtcatgtg gatatcaaca acgtggtatg tgcgatgcct ttgccctata
    40681 cccgcttgta cgaaggttta gcgagcggat tgaaagccca gtttgatggc attccttttt
    40741 actatgcgca tatgcgcggc gcggatattc gcgtggagtg tttacttaac ggcgtgtatg
    40801 acatggcggt ggtttcccga ctggcggcgg aaagttatct cacgcaaaaa gggttttgcc
    40861 tcgcgctgga gttggggccg cacacctacg ttggcgagca ccagttgatt tgccgtaaag
    40921 gcgagtccgc aaacgtgaag cgcgtggggc tggataaccg ttcggcggat cagaaaatca
    40981 tgaccgatgt cttttttggc gatagtgatg tggaacgtgt cgatctctct tatcacgaga
    41041 gtttacaacg cattgttaaa ggcgatgtcg atgcggtgat ctggaacgtg gtggcggaaa
    41101 acgaactgac catgctggga ttagaggcga cgccgctcac agacgatccg cgatttttac
    41161 aggccaccga agccgtggtt ctgacgcgag tcgatgatta cccaatgcaa caactgctgc
    41221 gtgccgttgt agataaacac gccctgctcg cccatcaaca acgggtagtg agtggggaac
    41281 aggaaccgag ttattaaacg aaaggcatct gatatggaaa ccagactcaa cctgctttgc
    41341 gaggcaggcg ttattgataa ggacatctgc aaaggcatga tgcaggtcgt caacgtactg
    41401 gaaacagagt gccatctgcc ggtgcgcagt gagcaaggaa cgatggcgat gacacatatg
    41461 gcgagtgcac tgatgcgcag tcgccgtggt gaagaaatag agccgctgga taacgaattg
    41521 ctggcagagc tggcgcaatc cagccactgg caagccgttg tgcaattgca tcaggtgttg
    41581 ttgaaggaat tcgcactgga agttaacccg tgtgaagaag gctatttgct ggccaacctt
    41641 tatggattat ggatggctgc taacgaagag gtttgaagct cccgacaaat ccacgtcgca
    41701 caacctaaat attcaaaaaa atgaatattt aattcaagga acagaaacga ggcaacaaga
    41761 tgtttgtaga agcattgaaa cgccagaacc cggcgctgat ttccgccgta ctaagtttgt
    41821 ggcagcaggg caagatcgcc ccggacagct gggtgatcga cgtggatcag gtactggaaa
    41881 acggtaagcg actgattgag acggcgcggc tttacggcat tgaactgtat ctgatgacca
    41941 agcagtttgg ccgtaaccca tggctggcgg aaaaattgct ggcattaggt tacagcggca
    42001 ttgtggcggt ggattacaaa gaggcgcgag tcatgcgccg cgctggtttg tcggtggcgc
    42061 atcaggggca tctggtacaa atcccttgtc atcaggttgc tgacgccgtt gaacagggca
    42121 ccgacgtcat caccgtgttt actctcgaca aagcgcggga agtttctgcg gcagcggtga
    42181 aggccggacg tgtgcagttt gtgctgctca aagtttatag cgacgatgat tttctttatc
    42241 cggggcagga gagcggtttt gtacagcatt cgctgcacga ggtggttgct gaaattaaga
    42301 aactgccggg tctgcattta gccggactta cccatttccc ttgcctgctt tgggatgagg
    42361 ctgccgggaa agttttgccg acaccgaatc ttcacacgct ggtacaagca cgggatcaac
    42421 tggcgaaatc tggtattgcg attgagcaac tgaacgcgcc ttctgcgacc agctgcactt
    42481 cgctgccgat gctggcggaa tacggtgtga ctcatgccga acccggtcat gcgctgacgg
    42541 gcactattcc ggcaaaccag cagggcgatc agcctgaata tatagcgatg ctttggttaa
    42601 gtgaaatctc ccatcatttc cgtggcgaca gctactgcta cggcggcggt tactaccgtc
    42661 gtggtcatgc gcaacatgcg ctggtgttta cgccagaaaa tcaaaagatt actgaaacca
    42721 atctcaaaac tgtggatgac agcagtatcg actacaccct gccgctggca ggcgagtttc
    42781 cggtaagcag tgcagtagtg ctctgttttc gcacgcagat ttttgtcact cgtagcgatg
    42841 tggtgctggt gtccggtatt caccgtggcg aaccgaaaat cgtcggtcgt tatgacagtc
    42901 ttggaaactc tctgggggcg taatggcgcg atttgtggtg ttagtgattg atagctttgg
    42961 cgtaggggca atgaaagatg tcacgctggt gcgcccgcaa gatgccgggg cgaatacctg
    43021 tggtcacatc ctgagccagt tgccgcattt gcagctacca gcgctggaga agctggggct
    43081 aatcaacgca ttgggttatg caccaggtga tatgcaaccg tcagattccg caacctgggg
    43141 ggtggcagag ctgcaacatg aaggtggcga tacctttatg gggcatcagg aaattttagg
    43201 cacgcgcccg ttaccgccgc tgcggatgcc ctttcgcgat gtgattggcc gtgttgagca
    43261 ggcattagtt tccgctggct ggcaggtgga gcgccgtggc gatgatctgc aatttctgtg
    43321 ggtcaatcag gcggttgcga ttggcgataa tctcgaggcg gatttaggcc aggtctataa
    43381 cattaccgcc aatctctctg tgatctcttt tgacgacgcg atcaaaattg gtcgtatcgt
    43441 gcgtgagcag gtacaggtcg gtcgggtcat tacatttggc ggcttgttaa ccgacagtca
    43501 gcgtattctc gatgccgcag aaagcaaaga agggcgcttt attggtatta atgcgccgcg
    43561 ttctggcgct tatgacaacg gcttccaggt cgtgcatatg ggctacggtg ttgatgaaaa
    43621 agtgcaggtg ccgcaaaaac tgtatgaggc gggcgtgcca accgtgctgg taggtaaggt
    43681 ggcagatatc gtcagcaatc cttatggcgt gagctggcaa aacctggtgg atagccagcg
    43741 gattatggat atcaccctcg acgaatttaa cacgcatccg acggcgttta tttgcatcaa
    43801 tatccaggaa accgacctcg ctggtcatgc agaagacgtc gcacgttatg ccgaacgttt
    43861 gcaggtcgtt gaccgtaacc ttgcccggct tgttgaggcg atgcagccag atgattgcct
    43921 ggtcgtgatg gcggatcacg gtaacgatcc gaccattggt cacagccacc atacccgtga
    43981 agtggtgcca gtgctggttt atcagcaagg gctggtccac acgcaactcg gtgtacgcac
    44041 cacgctttct gatgtggggg ctaccgtgtg tgaatttttc cgtgcgccac cgccacaaaa
    44101 tggtcgctct tttctttcct ccctccggtt tgcaggagac accctatgag tattgatccg
    44161 acgggttaca ccctggccca tgagcatctg catattgatc tctccggctt taaaaacaac
    44221 gtggactgcc gccttgatca gtatgcgttc atttgccagg agatgaacga cctgatgacc
    44281 cggggcgtgc gtaatgtgat tgagatgacc aaccgttaca tggggcgcaa tgcgcaattt
    44341 atgctcgatg taatgcgcga gacggggatc aacgtggtgg cctgcaccgg ttattaccag
    44401 gacgcttttt tcccggagca tgtggcgacc cgcagcgtgc aggaactggc gcaggagatg
    44461 gtcgatgaaa ttgaacaggg tatcgatggc accgacctga aagccgggat tatcgcggag
    44521 atcggctcta gcgaagggaa gattacgccg ctggaagaga aggtgtttat tgccgctgcg
    44581 ctggcgcata accagaccgg acgcccgatc tccacgcata cttcgttcag caccatgggg
    44641 ctggaacaac tggctctgtt gcaagcccac ggagttgatc tttcgcgcgt caccgttggt
    44701 cactgcgatc tgaaagacaa tctcgacaac attttgaaga tgatcgatct cggcgcgtac
    44761 gtgcagttcg acaccattgg taagaacagt tactacccgg acgaaaagcg cattgcgatg
    44821 ctccacgcgc tacgtgaccg tgggttgctg aaccgcgtca tgctgtcgat ggatattacg
    44881 cgccgctccc atttaaaagc caatggtggt tatggctatg actatttatt gaccaccttt
    44941 attccgcaat tgcgccagtc aggattcagt caggccgatg tggatgtgat gttacgtgaa
    45001 aatccctctc aatttttcca ataaggacag actcatgaaa aagattggcg ttgcaggctt
    45061 acagcgtgag cagattaaaa aaaccattga agcgacggct cctggcagtt ttgaagtttt
    45121 cattcacaac gacatggaag cggcaatgaa agtgaaatcc gggcaactgg attactacat
    45181 cggtgcgtgt aataccggtg cgggcgcggc attgtcgatt gccatcgcgg tgattggcta
    45241 taacaaaagt tgcaccattg ccaaaccagg cattaaagcg aaagacgagc atatcgccaa
    45301 aatgatcgct gaagggaaag tggcgtttgg cctttccgtt gagcacgtcg aacacgcgat
    45361 tccgatgctg attaaccatc tgaaataaaa ggcacgacta tggatctgta tattcagatt
    45421 atcgtggtgg cgtgcctgac gggtatgaca tcgcttctgg cgcatcgctc ggcggctgtt
    45481 tttcatgacg gcatccgccc gatcctgccg caactgattg aaggctatat gaatcgtcgc
    45541 gaggcgggga gtatcgcttt tggtctgagc attggttttg tggcctcggt ggggatctct
    45601 tttaccctga aaaccgggct gctcaacgca tggttactct ttcttcctac cgatatcctc
    45661 ggcgtactgg cgataaacag cctgatggcg tttggtcttg gcgctatctg gggcgtgttg
    45721 atccttactt gcctgttgcc agtaaaccag ctgctgaccg cgctgccggt ggatgtatta
    45781 ggtagcctcg gggaattaag ctcgccggtg gtttctgctt ttgcactgtt cccgctggtg
    45841 gcgattttct accagtttgg ctggaagcaa agtctggtcg ccgccgtggt tgttctgatg
    45901 acccgtgtgg tagtcgtgcg ctatttccca catcttaacc ctgaatccat cgaaatcttt
    45961 attggcatgg tgatgctgct ggggatcgcg ataactcacg acctgcgtca tcgtgatgaa
    46021 aatgacatcg atgccagcgg gctttcggtg tttgaagagc gcacgtcgcg gattatcaaa
    46081 aacttacctt atatcgccat cgtgggagca ttgattgccg ccgttgccag tatgaagatt
    46141 tttgctggta gcgaagtttc gatcttcaca ctggagaaag cgtattccgc aggcgtgacg
    46201 ccggaacaat cgcaaacgct gattaatcag gcggctctgg cagaatttat gcgcggactg
    46261 ggttttgtgc cgttgattgc caccaccgcg ttagccactg gcgtgtatgc agttgcgggc
    46321 tttacctttg tttatgcggt gggctatctc tcgccaaatc cgatggttgc ggcggtatta
    46381 ggcgcagtgg ttatttcggc ggaagtcttg ctgcttcgtt cgatcggcaa atggctggga
    46441 cgctacccgt cggtgcgtaa tgcgtcggat aacatccgta acgccatgaa tatgctgatg
    46501 gaagtggcgc tgctggtcgg ttcgattttc gcagcaatta agatggcggg ttataccgga
    46561 ttctctatcg cggttgccat ttacttcctc aacgaatccc tgggccgtcc ggtacagaaa
    46621 atggcggcac cggtcgtggc agtaatgatc accggtattc tgctgaatgt tctttactgg
    46681 cttggcctgt tcgttccggc ttaaggaagg cacctatgaa gacgtttcct ctgcaaagcc
    46741 tgacgattat tgaggcgcag caaaagcagt ttgcgctggt ggatagcatt tgtcgccatt
    46801 tccccggcag cgagtttctt actggcggtg atttagggtt aactccaggt ctgaatcaac
    46861 cgcgtattac ccaacgggtg gagcaggtgc tggctgatgc atttcacgca caggctgcgg
    46921 cgctggtgca gggcgcgggg actggcgcga ttcgcgccgg gctggcggct ttgctcaaac
    46981 cggggcagcg tcttctggtg catgacgcgc ctgtttaccc gacgacacgg gttattattg
    47041 agcagatggg gctgacgctt attactgttg atttcaatga cctgtcggca ctgaagcagg
    47101 tcgtcgacga gcaacaaccg gatgcggcgc tggtgcagca tacgcgccag cagccgcagg
    47161 acagctacgt gctggcagat gtgctggcaa cgttgcgcgc ggcaggtgtt ccagcgttaa
    47221 ccgatgacaa ctatgcggtg atgaaggtgg cgcgaatcgg ctgtgaatgc ggcgcgaatg
    47281 tctcgacatt ttcctgcttc aagctatttg ggccagaggg tgttggtgca gtggtcggcg
    47341 atgctgatgt tatcaaccgt attcgcgcca cgctttactc cggcggtagc cagatccagg
    47401 gcgcacaggc gctggaagta ttgcgtggtc tggtgtttgc gccagtgatg cacgcggtgc
    47461 aggcaggggt atctgaacgg ttgctggctt tgcttaacgg tggtgcggtg ccggaagtga
    47521 aaagcgcggt gattgctaat gcgcagtcga aggtgttgat tgtcgagttt catcagccga
    47581 ttgccgccag agtgctggaa gaggcgcaaa agcgcggtgc cttgccttac ccggtgggtg
    47641 cagagtcgaa atatgaaatc ccgccgctct tttatcgcct ttccggaacg tttcgccagg
    47701 cgaatccaca atcagaacat tgtgcgattc gcattaaccc gaatcgcagc ggtgaagaga
    47761 cggtgctgcg gattttgcgt gagagtattg ccagtattta atgctggata gcaccgtcaa
    47821 gctaaattcc gtactgaacg gtcccctcgc ccctttgggg agagggttag ggtgagggga
    47881 acgtacggga tgtaaaattc aacgcgccag catcgccgga tgcgatgctg gcgcatctta
    47941 tccggcctac ggttaatgtc ttttattatc aatcgttaag gtgatgcgat tggcgcggca
    48001 gaagagttcg gagacgtgca ccggtttgtc attctgatca aaggcgattt tgctgatgcg
    48061 aaacaacggt tcgcccagtt cgcatttcag ccattttgcc tgcgggcggg tggcggcaaa
    48121 gatatcgatg gtctttttgt cgctgaccac tcgcgtgtcg aaacgctcct gaaataactg
    48181 ataagtggag cttccttcaa cgtaaatctc atcaaagtca ggataacgcg acagtgggat
    48241 ccaggaacta tcaataaaca gcggctcttt atcgagatac atcacccggc aaagatggaa
    48301 cacttcgctg ttgccaggga tgttcagctt ttcgcaaaac ggcgcggcgc tgactcgttc
    48361 ctgctcgatc actttctctt tcgtcgcctt gccttgtgag acgccaaaat cggtaaaacc
    48421 actgacggtc agcagggcgt tttcgacctt ctggctttgt acaaaggtgc ctttgccctg
    48481 ccagcggatc agtacgccgt ctgccactaa gtcgctgata gctttgcgaa tagtaatgcg
    48541 gctgacgtta tattgcgtac aaagctcgtt ttcggtaggg atctgttgcc cggcctggta
    48601 aaccccctgc gcgatatcat ccagcaaccg ctggcggacg gtagcgtaga ggagttgatg
    48661 agagtagcgg tccgtagctg acataatcga ccctgaaata aagaatgaat tactcggatt
    48721 atagggtcgt tttccggcaa gtaacagcac gttggcgtga agatgctcac gccaacgtta
    48781 tacctaccag gcaccgtggt actgaatggt tttcgccgcg cacgccgttc cctgcgccat
    48841 cgcctgcggt aatgtcatcc ccgcagacca gccgcaaagg aatccggcaa tgaacgaatc
    48901 tccggcaccc atggtgtcga taaccgtcac cggttcagga gcctgacgcc agaactgcgc
    48961 gccatcccag gcaatgctgc cgttttcacc cagcgtgaca atcaccacgc ctgcgccacg
    49021 ggcaacaatc gctttcatct tcagacgcag cgcttcgtct tcttgcggtg cggaggcaaa
    49081 ggcaaaatcg agatgcggca ccagtgtctg ccagagcgag ctgtcccact tgtcggagaa
    49141 gtcgaaagcg gtaagtttgc ccgcagcgtg cagctgtggg aatgcgtctt ccgcatgtcc
    49201 ccagattgcc gcgtgcacaa tgtcatactg cgccagccag gcgtaatcct cttcactcag
    49261 ggcaaagtcg gccatcacgc cttcggtgta gtcgccaaaa acgcgatcat tgtcgtgcag
    49321 ttccacctga gtttgtgcgg taacgccgtg tttcgtatgg acatggctga tatcgacgcc
    49381 catgcgggcg agatcctgct tcagctttgt gccgtagtca tcgtcaccca cccaggtaat
    49441 gcatcccggc tgtatgccgt agcgagtgca gtacaccgcc acattgaccg cattaccgcc
    49501 agaaaacgct ttattcagtt gcgggtagat atcgacgcag ttatcgccga ttgtcgccag
    49561 ggttttcatc tctcatcctc cggcagcagc gcgcgaaagc gttccagcgc ctggcgggca
    49621 tagagtctgg gctcgttcat atacatcgtc accagctcca ccgtacagta accctcatag
    49681 ccccggtcga taatatcgcg catcagttcc ctcagcggca ttttgccttc tccaggaatg
    49741 taatgcgtgt cgctggcccc gtcgctgtcg acaatatgca gatgacgtaa tttatcgccc
    49801 agtttgtcga aataactcat caccggttcc gcctggacat acggcgcgca aatgtcgacc
    49861 atgctgaaca ggcgcggcga aggcaccagc gccagcgcat gaagcacatc attagcatta
    49921 cacacgacgt tcgattcata cggcgttaac ggttcgagga ttagatccat gccgatgttt
    49981 tccgcgtact cacacagttc gcttaggttc tctgccagcc gtccccagat aacattaggt
    50041 ggcgtgagat agcccgcgtg ggccgcggaa atcagcgtat aacccgcgtt catctctttt
    50101 gccatatcca tcgccagctt gatcatgtcg aggctttcgc gacgcatatg ttcatcgccc
    50161 agcatcatgt tatacggata gccgttggtt tctggcgtat agccgataat cggcatctga
    50221 tacgtctgcg ccagcgcctt gatttgtttg atgccgcccg cttttaagtc cggcgcgaac
    50281 gcgtgcgggc gaccgcccca aatttcgatg ccgtcgtaac ccagctcgct tgcatcacga
    50341 aatgcgtgtt caataggcag ccgctggtgg ccgcaggtaa acatacctgt tttcatcggg
    50401 taactccttg cgtaaattcg gcccgttgcc gggccgggag agggattaat attccaccag
    50461 accaccgtaa tagcggcgtt catccgggtt gtgatctttg taaatagaca ggtagtagca
    50521 gagccactcc attggcacga acatcaggaa cggtgccagc cacgggtgca gcccttgcga
    50581 aatttcggcg taatcgatga cgatcacgtt gtcagtacgc tgtttaacaa agttaatggc
    50641 gcgttcggtg gtgtggcgac tttcatcatt gccgagcagg aacaggaacg gaacgcccgg
    50701 ttcgacaatc tccagcgggc catggcggaa ctctccgctc tcaatcacgc agccgtgcgt
    50761 ccaggtaaat tccatcagcg ttacaatgcc ttctttgtaa cccagcggac gcagcggacc
    50821 cgcagcaacg gtataaatca tcggccactg gctggccagt tcaccaagct ggcggccttt
    50881 ttcttcccag gtgcgtacca gttgaccgag cgcattcggc aactgcttga gatcgttttt
    50941 gatcttgccg atttccgcgt tcggcgcgag gcgggtgatc atctccagca ccacgctgta
    51001 gcagagcagc aggtgaattt cccagataca gtcggcctga taatcaatgc taaattccgc
    51061 cgccgaggta atcgggctgt ccgcgcgttt ggtgaacgcc gcagtgagtg cgccgcaggc
    51121 ccggcccagc tccagcgctt tgattacctc ttccgtttta ccgtagtcag aaacgccaat
    51181 caccgcgcaa cgatcgtcga ggcgatacgg ggtgttatcg cagaactccc agccggaaat
    51241 ggcgtagacc tgaagatcgg aaaagcgatc cgccagatgt ttcgccgttt gcgccgcgtt
    51301 gagtggcgat ccgcaggcaa cgaaataaat acgatcaatg tcgcgcttca ccatctcttc
    51361 cacgatggtg tgcaccagcg gaacgtcatg gctgagaact ttttccactt cctgaaccat
    51421 attttcggtg accagaaagt ccacggtgct tttatcaata tccaacattt tattttctcc
    51481 agataacatt acgacaaagc gttgagctgg cggctacgct tcgcccagaa ggcgtaagca
    51541 ggcagaccag tagcaataac gatgacggcg cagataaggc cgggaatcgg tgcccagaca
    51601 aaggttgagg cgaccagaat gaggcttgac gcaatggcga gggtggtcat cagcccgaaa
    51661 gccggagtac gccacagcgg tttgtaatcg tcgcgtttac gacaccagat gatggagccg
    51721 aaggtgaggg tatttttgaa acacatcacc agggtgaaat aacccagcag gctggtgaga
    51781 tcggaaacga agatgaagaa gatccccagc gccccttgca ggatgatgga gacatccggc
    51841 gtgttgtatt tcggatgcac atggccgaag catttaaaga acaggttgtc tttcgccatc
    51901 gcgtattcca gccgcggctg gtacatcacg cagctggaaa gcgaaccaag aatgacgatc
    51961 atcgccgtga tggcaacaaa gatcccagcg gtgctgccga gtgcggggat ccaggtcagg
    52021 gcgtcggaaa tcggcgtttc agaattggcg agtttgtcga agggcatcag gccggaaatc
    52081 accagcgcca gcagggtgta gagcaccaga accagcagac aggaaccaat cagcgctcgt
    52141 ggcatggttt ttccggggtt tttaatttcg ccggtcatat aacagataga ggccatgccg
    52201 gtatacgacc aactggtggc agagatcccc gccagcagcg ccataaagct gcccgttgcg
    52261 ccaatcgcag tggtggtagg ggcggcaaaa ttctccgctt taaaccagaa gatcccaagg
    52321 ccaatgacga tagtgaacgg gataattttg gcgatggtaa ttagcgtctg aaacgctgcg
    52381 ccgccttcaa cggagcgcag gtgtagcaac ataaaggcga taattaatcc ggcggcgata
    52441 aatttaccga gcaacggatc gataggcgtt aaaaagccaa gattgctgac aatcgccagc
    52501 gccataatcg acaatgacgg cgcatcgttg gcccagaagc tggcccagcc ggagaggaaa
    52561 gccagcggtc ggcttccggc atttttcaga taaacataat ctgcgccatt ttccggataa
    52621 gcggtggata gttccgcata gacgcacatt tgcgggatca caattaaccc gccaatgaca
    52681 aacgcgagca ccgtaagcca cggcgtgccc gctgcttttg ccacttcacc cacagataca
    52741 aaaataccgg agccgacggt tgtcccgacg gcgattgcaa gaacggccca aaatccgagc
    52801 ttgcgttgga gttcctggct tcccatagcg ataccttttt cttgtccaat acgttgtagg
    52861 ggagagtttg cagtcattta aaaggtaaca tgacaatgca tgatgaatat aacaacatat
    52921 gatgttatgt aatgtggagg aagatcacaa aatttcgcac aggatcgcgc tgtggctaat
    52981 ggatgtagtt atcaaattga atttaaagtg aaaatatttt tacgggcggg ggcaagaagg
    53041 acatagaaac aaatacgccc tcggaaaatc cagagggcgt cgggcaatta aaccggtgtt
    53101 agccgatttc tgtcagagac ttactgtgca gtaggaccgc aaccgccaat gattttagaa
    53161 atagaaattg ccgggtgcag caggtagtcg taagagcagt tcttatcacg gttttcaata
    53221 tgaccagtac atgcagttaa cgtagccagt actgcaccta ccagtgcaac tttaataaat
    53281 ttgttcattt acattcccta tgtattatat attaattaat attcattgat tatatgaata
    53341 taagcagact gctcgttaag aacgatacag gaatatttaa ttggcgtcag tgagcgaatg
    53401 acttaatt
//
LOCUS       DAZKGS010000013        52492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKGS010000013 DAZKGS010000000
VERSION     DAZKGS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(78..479)
                     /gene="crl"
                     /locus_tag="ACHOQY_000807"
     CDS             complement(78..479)
                     /gene="crl"
                     /locus_tag="ACHOQY_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9676954.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(537..1781)
                     /gene="frsA"
                     /locus_tag="ACHOQY_000808"
     CDS             complement(537..1781)
                     /gene="frsA"
                     /locus_tag="ACHOQY_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9676955.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(1873..2331)
                     /gene="gpt"
                     /locus_tag="ACHOQY_000809"
     CDS             complement(1873..2331)
                     /gene="gpt"
                     /locus_tag="ACHOQY_000809"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9676956.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2592..4049
                     /gene="pepD"
                     /locus_tag="ACHOQY_000810"
     CDS             2592..4049
                     /gene="pepD"
                     /locus_tag="ACHOQY_000810"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9676957.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4106..4720)
                     /gene="prfH"
                     /locus_tag="ACHOQY_000811"
     CDS             complement(4106..4720)
                     /gene="prfH"
                     /locus_tag="ACHOQY_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP9676958.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4717..5856)
                     /locus_tag="ACHOQY_000812"
     CDS             complement(4717..5856)
                     /locus_tag="ACHOQY_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP9676959.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(6102..6554)
                     /gene="yafP"
                     /locus_tag="ACHOQY_000813"
     CDS             complement(6102..6554)
                     /gene="yafP"
                     /locus_tag="ACHOQY_000813"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9676960.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(6551..7606)
                     /gene="dinB"
                     /locus_tag="ACHOQY_000814"
     CDS             complement(6551..7606)
                     /gene="dinB"
                     /locus_tag="ACHOQY_000814"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9676961.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7677..8600)
                     /gene="lafU"
                     /locus_tag="ACHOQY_000815"
     CDS             complement(7677..8600)
                     /gene="lafU"
                     /locus_tag="ACHOQY_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9676962.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPQVQKLDKQQVLSQRTR"
     gene            complement(8603..9466)
                     /gene="motA"
                     /locus_tag="ACHOQY_000816"
     CDS             complement(8603..9466)
                     /gene="motA"
                     /locus_tag="ACHOQY_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9676963.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(9479..10195)
                     /locus_tag="ACHOQY_000817"
     CDS             complement(9479..10195)
                     /locus_tag="ACHOQY_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFP9676964.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(10215..10682)
                     /locus_tag="ACHOQY_000818"
     CDS             complement(10215..10682)
                     /locus_tag="ACHOQY_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFP9676965.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(10690..11751)
                     /locus_tag="ACHOQY_000819"
     CDS             complement(10690..11751)
                     /locus_tag="ACHOQY_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9676966.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(11748..12059)
                     /locus_tag="ACHOQY_000820"
     CDS             complement(11748..12059)
                     /locus_tag="ACHOQY_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFP9676967.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(12064..12456)
                     /gene="fliS"
                     /locus_tag="ACHOQY_000821"
     CDS             complement(12064..12456)
                     /gene="fliS"
                     /locus_tag="ACHOQY_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9676968.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(12479..13795)
                     /gene="fliD"
                     /locus_tag="ACHOQY_000822"
     CDS             complement(12479..13795)
                     /gene="fliD"
                     /locus_tag="ACHOQY_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9676969.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(14002..14916)
                     /gene="lafA"
                     /locus_tag="ACHOQY_000823"
     CDS             complement(14002..14916)
                     /gene="lafA"
                     /locus_tag="ACHOQY_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFP9676970.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            15402..16247
                     /locus_tag="ACHOQY_000824"
     CDS             15402..16247
                     /locus_tag="ACHOQY_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP9676971.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDDGKQQNIIKTIPKKGYLCNKE
                     YVSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGST
                     VGYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLS
                     AHNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLN
                     TLQKC"
     gene            complement(16302..17279)
                     /locus_tag="ACHOQY_000825"
     CDS             complement(16302..17279)
                     /locus_tag="ACHOQY_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFP9676972.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(17296..18225)
                     /gene="flgL"
                     /locus_tag="ACHOQY_000826"
     CDS             complement(17296..18225)
                     /gene="flgL"
                     /locus_tag="ACHOQY_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9676973.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(18240..19616)
                     /gene="flgK"
                     /locus_tag="ACHOQY_000827"
     CDS             complement(18240..19616)
                     /gene="flgK"
                     /locus_tag="ACHOQY_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9676974.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(19798..20097)
                     /locus_tag="ACHOQY_000828"
     CDS             complement(19798..20097)
                     /locus_tag="ACHOQY_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFP9676975.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(20097..21197)
                     /locus_tag="ACHOQY_000829"
     CDS             complement(20097..21197)
                     /locus_tag="ACHOQY_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9676976.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(21212..21877)
                     /gene="flgH"
                     /locus_tag="ACHOQY_000830"
     CDS             complement(21212..21877)
                     /gene="flgH"
                     /locus_tag="ACHOQY_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9676977.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(21960..22745)
                     /gene="flgG"
                     /locus_tag="ACHOQY_000831"
     CDS             complement(21960..22745)
                     /gene="flgG"
                     /locus_tag="ACHOQY_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9676978.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(22824..23561)
                     /locus_tag="ACHOQY_000832"
     CDS             complement(22824..23561)
                     /locus_tag="ACHOQY_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFP9676979.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(23561..24763)
                     /gene="flgE"
                     /locus_tag="ACHOQY_000833"
     CDS             complement(23561..24763)
                     /gene="flgE"
                     /locus_tag="ACHOQY_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9676980.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(24787..25500)
                     /locus_tag="ACHOQY_000834"
     CDS             complement(24787..25500)
                     /locus_tag="ACHOQY_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFP9676981.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(25500..25931)
                     /gene="flgC"
                     /locus_tag="ACHOQY_000835"
     CDS             complement(25500..25931)
                     /gene="flgC"
                     /locus_tag="ACHOQY_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9676982.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(25934..26269)
                     /locus_tag="ACHOQY_000836"
     CDS             complement(25934..26269)
                     /locus_tag="ACHOQY_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFP9676983.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            26351..27088
                     /gene="flgA"
                     /locus_tag="ACHOQY_000837"
     CDS             26351..27088
                     /gene="flgA"
                     /locus_tag="ACHOQY_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9676984.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            27169..27447
                     /gene="flgM"
                     /locus_tag="ACHOQY_000838"
     CDS             27169..27447
                     /gene="flgM"
                     /locus_tag="ACHOQY_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9676985.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            27460..27888
                     /gene="flgN"
                     /locus_tag="ACHOQY_000839"
     CDS             27460..27888
                     /gene="flgN"
                     /locus_tag="ACHOQY_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFP9676986.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            28015..28944
                     /locus_tag="ACHOQY_000840"
     CDS             28015..28944
                     /locus_tag="ACHOQY_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFP9676987.1"
                     /translation="MSFIECYEPEYVRKFLARPPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            29123..31567
                     /locus_tag="ACHOQY_000841"
     CDS             29123..31567
                     /locus_tag="ACHOQY_000841"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9676988.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(31685..32080)
                     /locus_tag="ACHOQY_000842"
     CDS             complement(31685..32080)
                     /locus_tag="ACHOQY_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFP9676989.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(32083..32517)
                     /gene="fliJ"
                     /locus_tag="ACHOQY_000843"
     CDS             complement(32083..32517)
                     /gene="fliJ"
                     /locus_tag="ACHOQY_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9676990.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(32520..33857)
                     /gene="fliI"
                     /locus_tag="ACHOQY_000844"
     CDS             complement(32520..33857)
                     /gene="fliI"
                     /locus_tag="ACHOQY_000844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9676991.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(33850..34560)
                     /gene="fliH"
                     /locus_tag="ACHOQY_000845"
     CDS             complement(33850..34560)
                     /gene="fliH"
                     /locus_tag="ACHOQY_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9676992.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(34563..35573)
                     /locus_tag="ACHOQY_000846"
     CDS             complement(34563..35573)
                     /locus_tag="ACHOQY_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9676993.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(35551..37197)
                     /gene="fliF"
                     /locus_tag="ACHOQY_000847"
     CDS             complement(35551..37197)
                     /gene="fliF"
                     /locus_tag="ACHOQY_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP9676994.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(37202..37543)
                     /locus_tag="ACHOQY_000848"
     CDS             complement(37202..37543)
                     /locus_tag="ACHOQY_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9676995.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(37558..38544)
                     /locus_tag="ACHOQY_000849"
     CDS             complement(37558..38544)
                     /locus_tag="ACHOQY_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFP9676996.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            38931..39782
                     /locus_tag="ACHOQY_000850"
     CDS             38931..39782
                     /locus_tag="ACHOQY_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP9676997.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            39775..40146
                     /locus_tag="ACHOQY_000851"
     CDS             39775..40146
                     /locus_tag="ACHOQY_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP9676998.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            40143..40895
                     /gene="fliP"
                     /locus_tag="ACHOQY_000852"
     CDS             40143..40895
                     /gene="fliP"
                     /locus_tag="ACHOQY_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9676999.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            40898..41170
                     /locus_tag="ACHOQY_000853"
     CDS             40898..41170
                     /locus_tag="ACHOQY_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFP9677000.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            41172..41954
                     /gene="fliR"
                     /locus_tag="ACHOQY_000854"
     CDS             41172..41954
                     /gene="fliR"
                     /locus_tag="ACHOQY_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9677001.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            41944..43083
                     /gene="flhB"
                     /locus_tag="ACHOQY_000855"
     CDS             41944..43083
                     /gene="flhB"
                     /locus_tag="ACHOQY_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFP9677002.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            43067..45160
                     /gene="flhA"
                     /locus_tag="ACHOQY_000856"
     CDS             43067..45160
                     /gene="flhA"
                     /locus_tag="ACHOQY_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9677003.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            45265..45543
                     /locus_tag="ACHOQY_000857"
     CDS             45265..45543
                     /locus_tag="ACHOQY_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9677004.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            45536..45892
                     /locus_tag="ACHOQY_000858"
     CDS             45536..45892
                     /locus_tag="ACHOQY_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9677005.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(45949..46722)
                     /gene="yafL"
                     /locus_tag="ACHOQY_000859"
     CDS             complement(45949..46722)
                     /gene="yafL"
                     /locus_tag="ACHOQY_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9677006.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            46908..47168
                     /gene="dinJ"
                     /locus_tag="ACHOQY_000860"
     CDS             46908..47168
                     /gene="dinJ"
                     /locus_tag="ACHOQY_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP9677007.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            47156..47443
                     /gene="yafQ"
                     /locus_tag="ACHOQY_000861"
     CDS             47156..47443
                     /gene="yafQ"
                     /locus_tag="ACHOQY_000861"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFP9677008.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            47599..48339
                     /gene="dpaA"
                     /locus_tag="ACHOQY_000862"
     CDS             47599..48339
                     /gene="dpaA"
                     /locus_tag="ACHOQY_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9677009.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(48310..49077)
                     /gene="yafJ"
                     /locus_tag="ACHOQY_000863"
     CDS             complement(48310..49077)
                     /gene="yafJ"
                     /locus_tag="ACHOQY_000863"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9677010.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(49283..49861)
                     /gene="lpcA"
                     /locus_tag="ACHOQY_000864"
     CDS             complement(49283..49861)
                     /gene="lpcA"
                     /locus_tag="ACHOQY_000864"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9677011.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            50101..>52492
                     /gene="fadE"
                     /locus_tag="ACHOQY_000865"
     CDS             50101..>52492
                     /gene="fadE"
                     /locus_tag="ACHOQY_000865"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9677012.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEEL"
BASE COUNT        11500 a        14443 c        13954 g        12595 t
ORIGIN      
        1 gatgatattg tcgcggttgg catgacctat cagttttaat gattattgcc ggatgtgatg
       61 catccggcac atttcactca cgccgttaac ttcaccggct cgtcacgaaa atcatccgcc
      121 ggttccagct tcagattcag cgtcgtcagc agttcacgca gcttctcgtg aaactcacgc
      181 agggtgtgct ccagtcgttc aaccacttca gtgtctttta ccggaacact cttccagtcg
      241 cctgctttat cgaacagacc aaactggtaa ctgtaggtaa aacgggattc ctgcgcttca
      301 agctccatcc accagcccca gaactcacgc acttccggtg ccggtttcac gttgacgcat
      361 acagccagac aatcgaaaaa gaatcgatta tcttcgcact taccttcacg aatatacggg
      421 cctagtgcgg taaatttttt gatcaatctg ctcttcgggt gtccactcgg taacgtcatt
      481 gctatctcct gttgtgatgc aactgtttta ccaaattggc aaaatttagc aaatttttaa
      541 cacaagcgtt tttcgatcca gccggtgatt tcctgaagac ctttgtcaaa attccgatac
      601 accgggttaa atgggatctc taataattta ccgtcagcag atgatgaggt gattaagcgt
      661 gagtcctctt ccgggctgaa cggatcgttc ttccagtagc ctgataacat tggcgttggg
      721 cagcgacgcc ccagcaatcc ttgcactttt aatgaatagc gattcagctc cacgcgtaac
      781 gcgtcatcgg aagcatcatg catccccaaa cgactcgcca gaacgtcaag atacatttcc
      841 ggcacctgtt gctggcactt aaaatcactc aacagggtat gaactaccgg accaagacag
      901 gcaaccgctt tcagacgcgg cgattcaaga tatgccagac gcacggcaac gttagcgccg
      961 aaacggaaac caaaggccgc gacgcgagtg tgatccaccc acggtacgtt aggcagcgcc
     1021 tttaagacgt gctgatgcaa caggctggag tcctgggtga gcttccattt tgaagaaaag
     1081 cccaccgatg gcatatcaat agtcagcatc gcaatgccgc gcggcgcaaa ataacgttca
     1141 tacaggctgt aatagtccgt ctgcatcgca tccagaccac cacacattaa tactgtcggg
     1201 aacgggccat cgcctttcgg catatgcaaa aagccggtga tgggcgcacc gccgggtacg
     1261 gtaaactcca tctgccgcat cgtgcccggc agacgctggg cggcctcttc ataggcgcgg
     1321 tttgataaag cctgcgcttg ctcggccagg tcatctcctt tcagatgagg ataggcggca
     1381 atgttgtaca acgtagccgc atgcagccag tgtcgaccac tgagttgcgg atcttcttcc
     1441 gcacaggctt tttgttgcca caccatcgcc tgggtggccc actcataaat ccagttgcca
     1501 ccacggtagc caatcacagt atcgtataaa tcatcgtcgg tacgttcagc atcgctcatc
     1561 acaatacgtg cctggacgtc gaggatttca cgtgggtcaa taccgcgcca gatccacatc
     1621 agacggttaa tcatgcgata ccagtgaggg atggttttac catcaagagc cgactgcaca
     1681 ggcggttgtg cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg atgtttaaag
     1741 cggggtttga acagggtttc gctcaggttt gcctgtgtca tggatgcagc ctccagaata
     1801 cttactggaa actattgtaa cctgcctgaa gttaaaaaga acaacgcccg gcagtgccag
     1861 gcgttgaaaa gattagcgac cggagattgg cgggacgaat acgacgccca tatcccacgg
     1921 ctgttcgatc caggtatctt gcgggatatc aacaacatag tcatcaacca gcggacgacc
     1981 agccggtttt gcgaagatgg tgacaaagtg cgcttttgga tacatttcac gaatcgcaac
     2041 agcagtacca ccggtatcca ccaggtcatc aataacgatg aagccttcgc catcgccttc
     2101 tgcgcgtttc agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg aaatacaaac
     2161 ggtatcgaca tgacgaatac ccagttcacg cgccagtaac gcacccggta ccagaccgcc
     2221 acggcttacg gcaataatgc ctttccattg ttcagaaggc atcagtcggc ttgcgagttt
     2281 acgtgcatgg atctgcaaca tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt
     2341 cccagcctgt ttatctacgg cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt
     2401 atagagatct ggcgcactaa aaaccagtat ttcacatgag tccgcgtctt tttacgcact
     2461 gcctctccct gacgcgggat aaagtggtat tctcaaacat atctcgcaag cctgtcttgt
     2521 gttgacaaca ttttctgcta accctgtgac ctgcaatact gttttgcggg tgatcgacaa
     2581 ggagacttaa cgtgtctgaa ctgtctcaat tatctccaca gccgctgtgg gatatttttg
     2641 ccaaaatctg ttctattcct cacccgtcct atcatgaaga gcaactcgct gaatacattg
     2701 ttggttgggc aaaagagaaa ggtttccatg tcgaacgcga tcaggtaggt aatatcctga
     2761 ttcgtaaacc tgctaccgca ggtatggaaa atcgtaaacc ggtcgtctta caggcccacc
     2821 tcgatatggt gccgcagaaa aataacgaca ccgtgcatga cttcacgaaa gatcctatcc
     2881 agccttatat tgatggcgaa tgggttaaag cgcgcggcac cacgctgggt gcagataacg
     2941 gcattggtat ggcgtctgcg ctggcggttc tggctgacga aaacgtggtt cacggcccgc
     3001 tggaagtgct gctgaccatg accgaagaag ccggtatgga cggtgcgttc ggcttacaga
     3061 gcaactggtt gcaggctgat attctgatta acaccgactc cgaagaagaa ggtgaaatct
     3121 acatgggttg tgcggggggt atcgacttca cctccaacct gcatttagat cgtgaagcgg
     3181 ttccagctgg ttttgaaacc ttcaagttaa ccttaaaagg tctgaaaggc ggtcactccg
     3241 gcggtgaaat ccacgttggc ctgggtaatg ccaacaaact actggtgcgc ttcctggcgg
     3301 gtcatgcgga agaactggat ctgcgcctta tcgatttcaa cggcggcaca ctgcgtaacg
     3361 ccatcccgcg tgaagccttt gcgaccattg ctgtcgcagc tgataaagtc gacgtcctga
     3421 aatctctggt gaatacctat caggagatcc tgaaaaacga gctggcagag aaagagaaaa
     3481 atctggcctt gttgctggac tctgtagcga acgataaagc tgccctgatt gcgaaatctc
     3541 gcgatacctt tattcgtctg ctgaacgcca ccccgaacgg tgtgatccgc aattccgacg
     3601 tggcaaaagg tgtggtcgaa acctccctga acgtcggtgt ggtgaccatg actgacaata
     3661 acgtagaaat tcactgcctg atccgttcac tgatcgacag cggtaaagac tacgtggtga
     3721 gcatgctgga ttcgctgggt aaactagctg gcgcgaaaac cgaagcgaaa ggcgcatatc
     3781 ctggctggca gccggacgct aattctccgg tgatgcatct ggtacgtgaa acctatcagc
     3841 gtctgttcaa caaaacaccg aacatccaga ttatccacgc gggcctggaa tgtggtctgt
     3901 tcaaaaaacc gtatccggaa atggacatgg tttctatcgg gccaactatc accggtccac
     3961 actctccgga tgagcaagtt cacatcgaaa gcgtaggtca ttactggacg ctgctgactg
     4021 aactgctgaa agaaattccg gcgaagtaat tatttgattt gctgccggat ggcgtttaat
     4081 cgccttccgg cagtttcatc cttcattatc cttcgataaa agccatccct gtaaatgtcc
     4141 gtctcgggtt gccacgttca atctggtgat ggaacattcg ccgcagcgat ttcagcaccg
     4201 cgctattttc ctgttgctgc tgctccagct tccaggcaat cagcaatcgt gccagccgct
     4261 tgttagcatg ctgactacgc tctgactgaa ccttcacgct aataccggat gccaaatgcg
     4321 tggcgcgtac cgccgagtcg gttttattga catgttgacc gcccggcccc gacgaacgca
     4381 acgtctcata acggattgca tccgattgtt cctgctcatc agcggtaaaa cgcccaatgc
     4441 ccagaaacca gtttttgcgc ccatgatgag gccgatacgg actcggacaa atccactgaa
     4501 tagtgccaca ccagctttcg cttaatgccc atgcgttatc gccatccaga gaaatcagcg
     4561 ccgaacgcag cgtgtcagag tagtggcccg tttctgtttc cagcaccgtt accgcgacgt
     4621 cttgtcgggc agcttcttta attagcctgt ccagcgcctt tttcactgcg agacaacatt
     4681 cttccggccc ctgcgctgag gagagttgta gcaagatcat ccttttttcc ctccactgtt
     4741 tttgagcgtt agcaccggac gcagccgcgc gacaggaata attaaaccgg cctgcaccag
     4801 acattgcacc acgctttcag ccgatttata ggcttgtggc gcttcttcaa agatgagttg
     4861 tttatcgcga caaattaccc gactgccaag ttcagtacgc gagagttgcg tcgccgtgta
     4921 tttcgctgcc agacgccctt tacactcggt gcgcccccat ttacgccctg ccccatgcgc
     4981 cagcgagtgg agcgtttttt cgttcgcaac aggctgtaca atccaggagt aatcaccacg
     5041 tgaaccggga ataatcacca gaccgttgtc atccggcgtg gcacctttac gatgcaacca
     5101 gccctgttga tcgccgattt gacacgcgct aacaaagtta tgcgccacat ccagaaccgg
     5161 acttcccgtg gccttaactt gttgcattat gcgcaaagcg atcatctggc gattaataca
     5221 cgcaaacgcc agcgcatcgt catgttccgc aatataccgt agcgcgtcgt cactgccttg
     5281 aggcaaacca tgatgcgaga acgaggcaat atgccgctgt aaaatagact gccccacccc
     5341 ccgcgagccg ctatgaacca acagttgcag atgttgtgca tccagacctg ccagtgcaaa
     5401 cagttcagcg ttgatgattt gatcaacttg ttgcagttca gcgaaatgat tgccgccacc
     5461 gatagagcca agcgagctgc gccacggatg ctgtgcaaat gctgacggca ggttttcctc
     5521 cagccagctt tcttcagcaa cgtcatccag atctgataat cgcttttcaa acttatcggc
     5581 gttgtatttg cgagcgagaa tatctgtttg ccatagcgcc ataccgcagc cgatatcatt
     5641 gccgaccagt gccggataaa aacgaccaac agagaagaac gctgcgccaa tcggatagcc
     5701 gcgtccggga tgtaaatctg gcatccccac tacgcgctgc atgttgggta aatttgccgt
     5761 ggtgtgtaat tgttggatcg ctaaactctc gatccacagg tcatcagatg cgatggtaaa
     5821 taccgcatcg gataagggac gaatataatt gcccatatac cattccgtaa atagactcgt
     5881 catacttcaa gttgcatgtg ttgcgacttg aattattgag agtaacaatt aaaaaatcag
     5941 gaaatggcag acgaatccag ggcaagaaaa aaagaattaa tcctggaaag gtctgcaaag
     6001 agtcattgat aaaggatttg tcatgattta cctccttgca gtttgtggaa taagaaaacg
     6061 cgcacatact aggcttgcgt tattttcatt gcaagctgga tttaatgttg ctgtttataa
     6121 cgcatataaa aattaataaa ccactctccc cgacattcaa cgcgttgctg cttaactgtc
     6181 tgaaaaccat aacgttcaaa aaagggtttt gcggttatgc ttgcgtccac cgtaagttcg
     6241 gattcagact taatcaaagg ttttaacaaa gcgctggcaa ccccacggcg ggtgtattca
     6301 gggtcaacaa ataacatatc gatatagtgt tcaacgcagg taataaagcc gaccagtttt
     6361 gcattaatga ctgcaacccg cacttgtgat ttcgcgagtt tctccttcca gcgagattcg
     6421 tcaatctgcg cccaggcggc aatttgttgt ggtgaataat gctgactggc agtcatcgta
     6481 accgctctaa tgaaaatagc gcatagttgc tgaaaatcgc caggctgata gtctcttatt
     6541 tgtatgttat tcataatccc agcaccagtt gtctttccat ttgcgggtca agcaacgtca
     6601 catgcagccc caccagtcgc acaccgcgcc cgccgcggcg ttcatcccag gttttacgcg
     6661 cggtggcgat taagtcagct ttattcagcc gcggccagac gtgctcctga gtggtttgct
     6721 gaaaatcatc aaactttaat ttcactccct gacgggcgat cagtaaatca ggttttacct
     6781 ttgccagacg gcgttcaagt tccggataca gccgctcgat aatcgcttca cattcagacc
     6841 agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac ggattttcgt aaccgttcgc
     6901 tgttaacgtc gcgctcgtca atcccctgac tacgctccca caaaatgcgg ccaaatttgc
     6961 caaagcgttt aagcagcatc accagatcac acttttgtac atcaccgcag gtccgcagcc
     7021 ccatcgcttc cagttttgcc gccgagactt tgccgacgcc ggggattttt gccagcggta
     7081 aggtttgtaa aaatgccgga acttctgccg gcgtaatcac aaactgaccg ttgggtttat
     7141 tcatgtcgga ggcgattttg gcgagaaact ttactggtgc cacgcccgca gacgctgtca
     7201 gttgcagctc gctgaaaatc gtctggcgga tttcctgggc gatgagggtc gcagaaccgt
     7261 ggcaatggac gctatcggtg acgtcgagat aagcctcatc cagtgacaac ggttcaatac
     7321 gcgaggtgta gcgcgagaag atttcacgga tatgatttga ggcttctttg taggcgtcaa
     7381 agcgccccgg aagcaaggtg agatgcgggc ataatttgag cgccatccct gtcggcatag
     7441 cgctacgtac gccaaattta cgcgcgggat aattggcggt gctgatcacc ccccgacgtt
     7501 cgcggctgcc gccaatagca atagggatat cgcgcagggc gggattgtcg cgcatctcca
     7561 ccgctgcgaa aaagcagtcc atatccacat gaatgatttt acgcattact cacctctcaa
     7621 cactggtaaa gtatacagtg atttcagggt ttgagaaatg cgtaaagatt cagcatttaa
     7681 cgcgttcgct gcgaaagcac ctgctgctta tccagctttt gcacctgcgg ctgcaccact
     7741 ttatccccat gctgaccaaa gtattgatac agcgtatcgg acgcactttt ggtcagcacc
     7801 ataatctcaa tgcgccggtt gcccgcgctt tgcggatttt tggaatccag cagcatctgg
     7861 tccgccattg cgcttacctg catcacttta tcttccggca ttccggcctc ttccagcacc
     7921 cgacgagccg aaagcgcgcg gtcacccgaa aggttccagt tgttgtagat attgtttttg
     7981 taagccatcg catcggtatg cccggtaata ataattttgt tatcgagcga gtcgaaaact
     8041 ggcgcgagct ccaccagcag cgttttaaag aacggcataa tctgcgcgct gccgcgttcg
     8101 aacatattgc gattctggtc gtctttaatc agtacgcgta atccctgggg aacaatctcc
     8161 atctccagat tcgcttccat atgcgcatca cgggcaatgg tgttgatact ggttgccagc
     8221 tcgccaagct cggtggcgga tttcttcttc agcagcgccg ttttctcatt cacatcacgc
     8281 gcttttttct ccgtctcttc cggcaccgcc acggtggcag gttttggcgg atgagacggg
     8341 ctgattttat ttaacggcga cagcccgcca ccgttaaaaa tcgactgccc gtgcagcgcg
     8401 gcgataatgc tttcacgctc ggatttactg acgctgttga caatccacag cgtcataaac
     8461 agcgccatca tcgccagggt aaagtcggcg aacgccacct tccacgcccc gccatgatgc
     8521 ccggcatgat tctttttgat ctgccgccgg acgatggtgg tttttgcccc acgctcgcgg
     8581 cggttgcccg tctttctcat gcttattctt gctccagcat cgcattcacc cacgcgtcga
     8641 ggttggcgaa ggtcggtttg gacgccagat gcagcagctt acgcccggca tccaccgcca
     8701 gcagcgtcgg tttgccgcct gcctgcgcca ccagcacagt gcgcacgcat tccagcaaag
     8761 aatgttctgc ccgcgcctgt tgttccatgg cgttggcgag cgggtccatc aggcagtagc
     8821 agataaagac gcctaaaaac gtccccacca gcgctgccgc cactttcagg ccgatcaggg
     8881 caatagagcc gtcaatcgac tgcatggtga tgatgatccc cagcactgct gcacaaatac
     8941 caaatcccgg catcgcttcg gcggtacgtt gcagcgagcg cgacggcgtg agcagcgact
     9001 cttccgcagt gtccagttcc tgatcgagaa tgccttccag ttcatgggcg tcgattttac
     9061 ccatcgccat caggcgaaag ttatcggcaa taaaggtcac cagccgcttc tgggttaaca
     9121 ccagcgggta tttctggaaa atggtgctct cttccggttg ctcaatatgt tgatccagca
     9181 tgcgcagacc accgttttgc accatttcga gcaactcata caggcacatt aatagctgac
     9241 gctgaaactc cggcccgagc tgttttttac tgatcacacc tttaatttga tgggcaatct
     9301 ctttcagcac atgtttcggg ttaccaataa tcatcgcccc gaaaccggca cccaaaataa
     9361 tgatcatctc cgccggttgc cagatagcca ctaattgccc gcccgcttca aaataaccgc
     9421 caaatacgca gccgagcacc accagtaaac ctaatatttt ttgcatgatt tcttcacttt
     9481 aattcggata gacaaaatca cgaattttct gcgcgatctg tttattcagt tgacaaattc
     9541 gggcctcggt tagccctaat accagcgcaa tctctttgag attcatctca tgctggtaat
     9601 acatggagag gatcagttgc tctttttcgc ttaattgtga aagcgcctgt tgcagcattt
     9661 gctgagtgac aaactgatct tccagctccc ggccaacaag cggaatgcct ggcccttcgc
     9721 cgttcagcag ttcgtccagg ctggcgagcg tttccgcccc ttccagttgc tgatattcga
     9781 gataatcttc atgtgagatc ccttccacgg ccagttctga ccactccggt tcgtgcccca
     9841 gttttttgcg cgtttcgcga atcagatctt tcatctgatg atatttctgt cgcagctggc
     9901 gcggtcgcca gtcgagagcg cgcagctcat ccaggatcgc gccgcgaata cggtgcacgg
     9961 catagcccgc aaaaccttcg tccggcagcc cgtagcggcg gatggcatta agcagcccca
    10021 ttaacgccgt ttgttccata tcctgacgat caataatgca attacactgc ggggcaagtt
    10081 gccgcaccac tttacgcacc aacggtaaat aggcctgcaa gtagtggctc tcctgctgcg
    10141 gggttaatac cggcgcggca gcaaattctt ctgactcgaa tacatcctgt agcatgttta
    10201 tatccgcctg gtgattactg gaagaccact tttttcacca gcaaatcctt atacggtgcg
    10261 ctcattttgc gacgctgaag atctttcttt aacgcgtcag aaatgagttt gcggatggca
    10321 gatattttca tgccgcgcaa atcttcaaat ttcatttccg tcaggcattc aaccgcaata
    10381 ctttgataca gcggctcctg atgttttatt ttttccgctt ctttgtcact ggcgaccacc
    10441 atcactaatt ccgccgacaa ataatggtcc gcgccgttgt tgtcatgcag cgtgacaatg
    10501 gtttccggca acgtcacaaa cacgcttttg ctttcatcca gggtttccac cgtttccgtc
    10561 tgcgcggcgg cagtcggttt tccttttccg gcataatgat gccagacacc ccatccagct
    10621 ccggccccca ccaccaacgc cagaacggcg ctgaccacgc ccgccaccac gattttcttc
    10681 atattgcctt tacactttga tcaaaacgga ttcgttctgt tgaaattgag gcgcatcatc
    10741 aagctgtaag gcggcactca cctcttcctg ctgatggccc gactgttgtt gttgctgcga
    10801 ctgcccttcg gaggaaacct gtacgttcac ctccataaaa ttttgcgccg tcagatgctg
    10861 gcggagatcg tcgctaaact gctgtaacgc gcggcaaact tcactttggt tcgcgccgat
    10921 gtgcaccatc agtttgccgt tgtcgagttg tacggcgatc tcgagcttgc caagcgacgg
    10981 tggatcgaga cggatggtgg agatctgctg ttgtttgttg agctgaaagt ggatctgatc
    11041 tttcaaaagc gtcgtcagtt tttcgcccag ttcgtccatc gaaagcgtgg ctggtttggc
    11101 gacaatctcc tgatgatcgc tgagcagctc cggcgtctta acctgcaaca ccgcacgcgc
    11161 aataaccggt tcactgtccg gcactttttc cgttttacgc tcgacctgcg ggcgcgcggt
    11221 cacacgggtt agttcagatt tcgggcgagc tggctgtgtt gagacgcgcg gctgcgtcgg
    11281 cgtaatggca tgtaaatctt cactagcgta agtcgccagt ctggcctgct gttcgggctt
    11341 cgcctgcggc aactgactga tcagttcctg caactgcggc ggcaacggcg tgagttcctg
    11401 ctgttgcgtc tggcgttgcg gcgcgttttg caccaccgct ttcgtcaatt gctggataac
    11461 cggcgatgtt gcgacgtttc gcggctgcgg cgtgtcctga tgcggcgcgg caggttgcgg
    11521 aagtaacagc gccattagcg cctgcatcgc caccggatcg ttctcctgcg ggcgcgtcgc
    11581 ctgttgctga ctggtttttg cgctatgaac gcgctccgcc accgccgcga ccgccatttt
    11641 cggcagggta aacgcgggcg cgttttcacc gctcaccggc ggtaaaatat ccgccttcaa
    11701 cgaagcagtt tccgcaagcg ttccgagcgt ggcaagcaat gtcggattca tgacttctcc
    11761 gaaagtaaat taacaattga gtaggccagt acgccttcct gatgccgctg atgctgttcc
    11821 atacgcgctt tcaacacttc tgcgcgctgc tcgcgccgtt taatcagggc gttaaacgcg
    11881 tcatacagtc gcgctttcac tgcatccaac tgcgccgcat cggtttccgc cgccgcgtaa
    11941 tcgtgaaact ggcgtatcaa ctgctgatgc agtgccggaa ggcgtcgcca gcgttgcttg
    12001 tcgagggctt cattcatcgc ctcgaccagt gcgaaaagtt tcctgcgttg tttttccatc
    12061 gtcttaaccc agcttgcccg aaagcccttc ccagccttcg cgcaggttgc ttaagatcag
    12121 catcacttca tcgattttct ctgccgacag ttcgccgctg gcctcataca atcggtaaac
    12181 gcagtggtca tagagtcgcg aaaggttgac caccagctcg ccgccagttt caaactccag
    12241 cgaactggtg agcgcattga gaatgtcgat gcatttattg atgctctggg ctttcttctc
    12301 gaaacgccgc ccttcaatat ggcttttggc gcgttccagt tcgtccatca ggccagaaaa
    12361 cagcaccagc accagttcca gaggcgatgc cgccgcagta cgcgccgcga gatcgatctc
    12421 tttatattgc gaatagccgt cctgtacttg gtacatcttt agggatattc cttctgagtt
    12481 acgcgaatgc cgccatgctg gctttcatgg tgtacacctc gaccagcgtg ttggtgtact
    12541 cctcgagata acggtcgtag ttggcgttat aggtatcagt aagctgatcg ccttcgtcct
    12601 gaattttgct catctgatcg tcaatgttct gctggcgcag ggtgatgatg ccgttgctgg
    12661 agtccgtgta ggtgttaata aggtcgtcca tctgcgccac catgctgttg tcgccaacga
    12721 aaatagaggt cagaccgtca ggatttttcg ccatttcgtc gttgaactgg tcggagtcaa
    12781 tctgtaagtg gccgtgagaa tcgagggtga tgccatagtc aacaatcgac acgccgttgt
    12841 agctggcatg ggcgatgtca tcgagctggt tcgccagtga actgagtccg gcgtcgcctg
    12901 caaatacccc ggcgctggta ctgtcatcac cgtgggtggt tagcgaatca accgtatcaa
    12961 tcaaggtgtt ataggcatcg acaaaggtct gtactttctc ctggctggcg ctggaatctt
    13021 cgctgatgtt aaaggtagtg agatcgctgt cagaatcgct gacttcggtg aaagtcatgg
    13081 tgacaccggg aatcacatca tcaaacgtat tgctgctgtt ggtgatctca ggccccgtgg
    13141 cgctgccaag atgaataatc gcgtcctgag cggaggaaac atccgtggca acgggcgcgc
    13201 tggtgctatc gttgctggca tcatgccccg ttacgctcac cgaaaacgcg ctctgcgccc
    13261 cggtgctatc cgaggtgagc ataatcgttg tggtgccgtc ggtttttacc agcgccgccg
    13321 acacgcccgg attatcatcg gagtcgttaa tcgcattcac cagttcactg gcgtcgataa
    13381 aaccgtcgcc gtcaccattc tgatctgccg ccgacagatc gatatccatg gtgctgtcgc
    13441 ccatcgtcag ttcaaaagtg ccggtggcgg aaaaggcgtc gtcgcccata ctgaacgtgg
    13501 tttgctgccc ctgtgccagt tgctcaacga aaaatgagta gcttcccgcc tgcgcctggg
    13561 aattggcgga aacgctcgcc gagtcattat tgctggtggc ggcaaacgtc accgggccgt
    13621 cggtatcgct gttcagcgcg tcaacggcgc tctgaaaatc gctcaacgct gaactgagcg
    13681 aatccaggcc gctgctttca gcatccagct ctgtttgctt ctcctgcaag tttgccgcct
    13741 gagtggcgac atccgcatac gcgatttctt gcgcaatggt tcttgggttg atcattgatg
    13801 actccggata acgatgtcag gttattggca aaaggcgtgc caatattttt tatgtgaata
    13861 atcaatagat taaaaaacag gcggaaatgg cgtttccggt ctggcggaaa aaagcctgcc
    13921 gcagaaagca caacaccgcc cgcaggcggt gttgttcgta gtccgaataa ggcgtttacg
    13981 ccgcatccgg caagtccgcc attactgcag caggctggaa accatgctgg acatgctgtt
    14041 ggactgtttc agcatggtga tgccagtctg catcagcacc tgctgtttgg tcatattgga
    14101 cgcttccgtc gcgtagtcgg tatccataat gttaccgata gcgacttcgg tgttgtcctg
    14161 catactggtc aggttgttgg cggtgtcgtt cagacggttg atagacgcac ccagcttgga
    14221 ctggatctgc gaaacgtcat ccatcgcggt agagatgctg gtgatcatct ggttagccga
    14281 gccagacgcg gtcagctcag taccgccaga aacacctgca gtacccgtgg tgtctgcctg
    14341 atccgcgcta aaggagttgc tgatagcaga aagatcggtc accagcgtgt tcagttggct
    14401 ggagatgttc acggtcatgg tgtcggaaga ttccgcgccc acctggaaag ttaccgcgct
    14461 ctggaacaga ccgtcagtac cggtgttgct ggtaccggaa acaccgaaca ggttggtgcc
    14521 gccgtaagtg gtgttttgca gcatgtcaga catctgctga cccagttcgt cgtattcgtc
    14581 ctgcatcgcc tgcagatcgt cgtcgctgta ggtgccgttc gctgcctgag ttgacaggtc
    14641 tttcatacga cccagcacat cagacatttc atcgaacata ctatcggcgg tttgcagcat
    14701 cgcggttgcg tcattgatgt tgctcagcgc cacgcccata ccgctggact gtgctgtcag
    14761 acggttagca atttgtttgc ctgcggcgtc atcggcagaa gagttaatcc ggttacctgt
    14821 cgccaggcgt tccatagatg tagacaaaga agagctgctt ttgctgattg cattaacggc
    14881 ggccattgag gcgttattgg tgttaataga taacatgata ctgctccttg ggattaatcg
    14941 ttttcgtttc gataccctgt aaaaacgaca cctaaggagc aaacttaaaa ccgccgttaa
    15001 aaaaatttca ctgcgattta tctgatgcca cttttagcta aaaaaagcag agtttaaatt
    15061 cagataaaaa gattattagc aatatctgcc acatggccag gtatcaaaat atgtctttat
    15121 caaacgcttg atatagttaa gattaccatc aattaacgcg ttttcttctc aatgtactga
    15181 tgccaaaatt tacattgcgt tgcaaatagt acacatttgg ctattgtacc agtcctgtca
    15241 tttcgttaca tttccatgcc atatagtgat taagaataat cctattacca taaaatatta
    15301 gttatcacgc agaaaaatca tcttctcagg gacgtataag actgtgcttt atgcaaagca
    15361 ctatcaggaa acatttatcc cttgctaagg taaactcaac tatgagtatt attatcaaca
    15421 actggcggat ggacccgtcg cttaatgcct taatacattg cgaaacgggt gaaacacgtc
    15481 gtctcggcga ataccatttt attttgctgg aaaaattagc aaaaaatgcc gatgtggttt
    15541 tatcgcgatc ttatttatgt gccgaagtat ggaagaaccg cattgtcggc ggaaacagcc
    15601 tgccgacggc gatccatgcg ttgcgcgttg ctatcgatga tgatgatggc aaacaacaga
    15661 acattattaa aacaattccg aagaaaggtt acttatgcaa taaagagtat gtgtctttgc
    15721 cggactcatc gcctgcaaaa aaactgatta ttaccgacca gatacaggaa acagtaccag
    15781 aagagatatc ttctacaaca ccgcctgtgc ctgttagaaa aaaacataaa gtaataatgg
    15841 ggctagcgtt agccgcggct gttatattta ttggctcgac cgtggggtat tcacacttga
    15901 aaagtacacc tgatgccccg caactggtaa aagagtcaat taacagtcca agaataaaaa
    15961 tatttcacct tagttctgga aaagaaaata attccgtacc gttactgtca caaaccctcg
    16021 caccaggaaa agacaaactc gataatttat tgtcagcgca taatatgaca atgacaacct
    16081 attataaata tgttcgtaac cgactggaaa gtgatattgt tctgcgcaac cagtgcaatg
    16141 gtagctggca attaactttt aatgtggaag gctggcagaa cagcgatatt aatagcgcga
    16201 tgtatcagaa tttagagaag ttgttaaata ctttgcagaa atgctgatga gtgccaatat
    16261 atgccggatg cgacgctaaa cgcgtcttat ccggctgata attaccccaa taaattcctc
    16321 accgtctggc tggaaattcg cgcctgacca ttcaccgcct gcaataaaac ctgatagtta
    16381 tgattcgcgc tatccagcac gccgccgagg ttttccgacg cctgcaccgc gttttccgtt
    16441 tgcgggaagc gcgacatgcc gtcgatcaac tgccgcgcct tttcctgctg taccgccatc
    16501 tgcgcacgct gctgattaat gctttctaac gtctgagaaa ttcccgcacc gccctgttgc
    16561 aggctttgag ctaaacggtc tgcctcagac ggctcggcaa aagttttcag cggcataaac
    16621 gccgacttat cgccatcggt gcgcacgctt aaggtacgtt cgatatgggg ccactgtttt
    16681 tcggtggtgg agaaactgat gccgtccgct tgctgatgta gctgcacgcc cacgcggcgc
    16741 agggcgttgg tcagccgcgt ctgatactgc gcggcgctgt catcctcaga cagcatcacc
    16801 gccgaaagct gagtctgccg cccgtcagag acgctgaaca tgcgcgttcc cggcgtggga
    16861 ttgtgtacca gattcgccag atccggcgag tgaaaggtca cccgcgcttc gccctgtaac
    16921 accggctgca actggcggtc caccgcaccg ccggaaagcg cggtgcgttt gtccagcatc
    16981 tgcatcagcg ccgtattctg cgcctgcccg cctttacgct ggctgtgacg atagtcgagc
    17041 aactgctgct ccagttgccc gagataatga tccgcctgct gcaacgtggt gagctggtcg
    17101 ttaagctgca cgctgtaacg ctgcgggcga gtggtaatca gcggcgaggc cggatattca
    17161 ctcgccgtcg ccgggagttt ttgccgcacc gcctgagtgg gcgcaacggt tgacgacgat
    17221 accgcagcgc tatgcgcgcc gccggtggcg agtgatgaag atgtaagtcc gacctgcata
    17281 ctgttcctga aagagttaga gaacgctgaa caaattcaac tcactcactt tcgagtaagt
    17341 cttgagggta acttccattg ccacttccag tccggtaaag gtgatcgacg ccgggccgta
    17401 atcgagatcc tgtaatgaac caatcagctc atcattagag gtggagatat cggtctgcgc
    17461 atcgctcagc atcgacatgg tattttgcgt ctcgccgagt gacgcaatcg aggcgttgag
    17521 gtcgtccgag gcggtgtcca ccacatcaac ggcattctga atatcgctct gcacctgcgg
    17581 atctgccggg tcgacgtccg ggttttgcaa ctcctgggat agggaattca gggtgttgag
    17641 cacgtctaaa ttgctgccaa agaaatcact cgccgccacg ttggtgtcca cttccacgcc
    17701 gttcgacacc gtggtctggc ggtggtcgct gttaccctga tagctgtagc tgtcggtgac
    17761 gccatcgccg tcatcgtcca ctgccacaat cggcggctga tcgttgatgg tgccgccaaa
    17821 cacgtagtgc ccgtcttcgt cctgataatt gagcgccgcc accattgatt cggtgagcga
    17881 ctcaatatcc tgcccgaggc ttgcgagtga atcggcggta ttggtgccat tcgccgcttc
    17941 cagcaggtcg tcgcgcaccg ccagcaggct gttgttgacg ccatcaagga tcgactcctg
    18001 ctggcttaag cccgccgacg cggagtcgat attgctctga tactgctcaa tcgccgactg
    18061 ctggcggttt aactgggtga ttcgcgtggc ggcgatcggg tcatccgacg gctgcaaaat
    18121 ctgcttgccg gttgccatct gctcgacaac atgcgccaga tcgccagaga gattattaaa
    18181 actttgcgtc atcgagacgt aggtttgttg ggtagttact cgcattttcg cgctcccgct
    18241 tagctgcaca tttccagcac cgaatcgaaa atctcggccc cggcggaaat gactttcaga
    18301 ttggcttcat aaatttgttg ataggtgatc aggttcaccg cctcttcgtc catgctgacg
    18361 ccgctgacgc tgctctgctg gttttgcgct gcggaataga cattggacgc ggcatcgact
    18421 tccgtctggt tttgctgact gtaaatgccg atattgctga tgattgacga gcacgcctgc
    18481 ccgaccgtca cgctgccgag gttggctatt tccagcggtt cggtggagat attgatcagc
    18541 gcctgcaggt tgtcgctgtt gccgctttcg tccggtgaac tggaaaacgc cagctcatcg
    18601 gcggtgatat tcgggttaac ggtcagcggg ccatcggcat tgctggcgtc ataaataaac
    18661 agcggctcgc cggggttacc gttgagatcg tagccctgcg ccagctggtt gttgaccgca
    18721 tcggcgaact gcgacgccat gctgttgatg gtgtcggtca gcggcgtcag cacatcgttt
    18781 tgataatcaa acagtgcgcc taatgaaccg ccggtgtcgg tggtcatcgt cgaggtggtg
    18841 ccagcgaagg tcagcgacat ggtcgacgtg ccatcggcgt tggtttccag cgcgatggtc
    18901 gaactttgct gcccactcac cagcggctga ccgtttttca gggtaacgtt gtagttgccc
    18961 tggtcgtcga tattgacctg cacatccatc atcccgctca gttcttccac catctggtcg
    19021 cgggcgtcgt acagcgcgga ggcgttatcg ccattggctt cagcctgagc gatttgctgg
    19081 ttatagctgg cgatgccgct ggtgagcgta ttgatttgcg acaccatcgc ctgctgctgg
    19141 ctgatgattt ccgtgctttg cgagtcgatg taatccagcg tgttatcaat acgcaacgac
    19201 agcgccccgg cttcactgat cacctgctcg cgcagggcgg aatcatcggg gctggtggtt
    19261 gcttcgttaa gggcggcgaa gaagttatcg aagccgccgc tcaggctgct gttatcgtcg
    19321 ctcagtaccg cttccagttg gctgagatcc ccctgctggg tgctgtaata gccgtaatcg
    19381 ctggcggcat accacacctg attcacctga tactggttgg agacgcggcg aatgctgtcg
    19441 acctgtacgc cgttaccggc gctgttcggc gaaccgccgc tggcaccgat ggtgctgatc
    19501 tccgccacct gacgggtgta gcccgtggtc atggcgttgg cggtgttttg cgccgtgacg
    19561 ttaagctcca cctgcgcggt tgatgcaccg ctgtagccga tgttaatcat gtccatgttg
    19621 gatccttacc ggtaaatcgt gccggttaaa agcggcgtaa agttggggat tcttaagttc
    19681 cgtaaaccgg gtgccgaacg ccggatgcgg cgtggacgcc ttatccggcc tacatacggg
    19741 cgactcgtag gcctgataag acgcgacagc gtcgcatcgt gcaccgaacg ccgaagctca
    19801 tttatgcttc ggcgacaact gcttcacaat catcgccgca atgccaatcc cgtgctgctg
    19861 cgccagggtg ttacacaact catcgtcata aaacccctgc atcatgcgca ccgaatcgct
    19921 gttaaacggg ttatcttttg aatcaaacaa ctcgttggtt ttccgcatct ctttcagcat
    19981 gttgcgtaaa aaaatcgcct caaactgttc ggcggcctgc ttgatatcat tggcctgaac
    20041 cttcggcccc attaacgcgt cgctgccgtc aatcccgccg ctggcgttca ctttcatcag
    20101 atcacctcca ggtcggcatc cagcgcgccc gcctcatgca gcgcctgcaa aatcgacata
    20161 atgtcgtccg gcgtcgcgcc taaactgttc agcgcattga cgagcgtttt caggctgctc
    20221 gactccggca aactcaccac gcccggacgg gcatgattca ccgcgatatt gctctgcggc
    20281 gtaaccgccg tgcgcccacc cgcaaaggca ttcggctggc tgacgttgct ggtttcgtta
    20341 atcgaaacgg tcaggctgcc gtgcgacacc gccgccgcgt gtagcgcaac gccatcgccc
    20401 attaccaccg tgccggtacg cgagttaaac accacgcgag cgcggatttt ctccgcctgc
    20461 acctgcacat cgtccagttg cgacataaat gccacccgcg cgccggggct ggtgggcgcg
    20521 cgcacggtaa cgttggtgga actctgcgcg gtggcaatac cgccaaacga ggcgttgatc
    20581 gccgccgcaa tgttgttggc gtctttaaac gacgggcgtt tcaggttgag ggtgatggta
    20641 tcgcccatct gaaaatcgct ggggatctcg cgctccaccg ttgcgccgtt aggaattaac
    20701 ccggcggttg gcgtattaac cgttacgctg gaaccgctgg ccccggtggc gttcatcccg
    20761 cccaccacca cgctgccctg tgccagggcg taaacttccc catcagcgcc gtgcagttgg
    20821 gtcagcagca aagtgccgcc gcgaatactt ttggcatcgc caatcgacga aacggtgaca
    20881 tcaatggtct gcccgcgcga gtacatcggc ggcagcgtgg cgctcaccgc caccgcggcg
    20941 acgtttttca ccttcgggtc gattttattc ggcagttgca cgccaaactg gcgcagcata
    21001 ttggtgatgg tctggttggt gaatttgacc tggtttttat caccggtgcc gtcgaggccg
    21061 accaccaggc tatagcccac cagttggttt tcgcgcacgc cctgtacgtt gaccagcgac
    21121 tccagcgatt gcgccagcgc cacaccaggc agcgccaggc tcacggcgat aactgcggcc
    21181 tttatccagt tttgcattgc actctccggc attaaatcgg gaacagagga tggttaaaca
    21241 gccgcgtcag ccagcctgcg gcgttggcgt cgcttaacgc gccgcgtccg gcataggaga
    21301 tgcaggcatc ggcgatccgc tgggaggaaa ccgagttgtc gttctggatg tcgtcggcgc
    21361 gcaccaggcc gcttaagcgg atgtactcat cgccctggtt gagggtgagc catttctcgc
    21421 cgcgaatttc caggatgccg ttgggctgca ccgcatggac ggaaacggtg atctccccgc
    21481 gcaggctgtt ctgctgctgg gaagtggcgc tgccgtcaaa gtcgcggttg gcgtcgatag
    21541 agccggaaag cttatcttta gtgtgactaa aaatggtcgg tgcgccgata tcgacggtgt
    21601 tgcttttgcc gaagttggtt ttcgcctgtt tgctcgactg ggtggattct tcgaggatca
    21661 ccgtcaggat atcgcccacg cgataagccc gccgatcggc ggtcagcgac cagttgtagc
    21721 cagtttcaaa tacaccgcca gcacgcccgt ctgccggagg cggtgcttcg gttttcggtg
    21781 gcgcaaagta tgcgtcgtct tttttgacca gaatggcctg cgattcacac ccgctaagca
    21841 ggggaagcag cgccaccagc cagagataat ttttcacgtt ctgcctgttt aaatacagtg
    21901 agttatgcgc ttttgtaggt cggataagac gcgtcagcgt cgcatccgac atttcggcct
    21961 tacagcgtct gactgatgta ctgcaacata tcgtccgcag ccgacaccat tttggcgttc
    22021 atctcgtaag cccgttgcac tgtaatcatc gccaccattt cgttgacgat atctacgttc
    22081 gagccttcga gggcattgtc ctgcaaagtg ccaagcccat cttcgcccgg cacaccttcg
    22141 gtcggctgac cgctggcggc ggtttccaga tacaggttgt caccctcagc ggaaagccca
    22201 gccgggttag cgaagttaac cagcgtcagt tgccctaact cggtcacgtc gctgtcgcca
    22261 ggaagaatcg ccgtcaccgt gccgtcctca ccaaacgcaa cattcgtcgc ccccgccgga
    22321 acgtcgattt ccggctgcaa tggcaagccc tgtgagttgg tcagtacgcc atcggcgttc
    22381 acctgcaaat tgccgtcgcg ggtataggcg atatcgccgt tggccttttg cacctgcaaa
    22441 aatccctgcc ccataatggc gacattcatc gcgttgtcgg tggtttcgac gttgccttcg
    22501 gtgaaggttt tctgggtgcc gacaatgcgc acgccgctac cgaattgcag cccggtcggc
    22561 atgatgttgt tctggtcgag catcgcgccc ggcgcttcct gggtctggta gaacagatcc
    22621 tgaaacatca cccggtcacg cttgaagccg gtggtgttaa cgttggcgat gttgttggca
    22681 atcgcgctca tttcagcatc ctgcgccgag aggccggttt tgctgatcca taatgctgcg
    22741 ttcatggtgg taaattccgt gttatttttg ggccgcggtt gccggatgcg gcgtgaacgc
    22801 cttatccggc ctacgatgtg cggttacgag ccgcgcaata agcggttgcc cgcgtcgctg
    22861 atatcttcgg cggttttcat catcttgatc tgtgcttcga actggcggtt catggcgatg
    22921 gacgacatca tttcgcttac cgccgagacg ttgctgccct cgagaaaacc gccgctgact
    22981 ttgatgtttt catcgcgctg ggccggaacg ccgtcggcag tcaccagcat cccttcagca
    23041 tttttctcca ggttagccac cgggatatcc accagcttca ggcgatcgat atccatcgtc
    23101 gcggtcacat cgccgtcatc gggcgtcacc gacagcgtgc cgtcgctgcc aaacgaagca
    23161 atggcgttcg gcggcagaat gatcggcccg ttatccccca gcaccagatt gccgtcgatg
    23221 gtcagttgcc cctggtcgtc ctgctgaatg ttgccgttgc gggtatagac ttcgttaccg
    23281 tttttatcct gcaccgcaat gtaacccgcg ccctgaatcg ccacgtccag cgggcgttcg
    23341 gttttctccg caacacccgt gctgtcgttc acgccgctgg ccccttcctg cgccatatag
    23401 cgcgtgtcga agccgccgcc tttcaccttg tcgttggttg ccattgccat atcggcgcga
    23461 aaaccattgg tgttgacgtt cgccaggttg ttggcgctaa tttgctgctc atacagggtc
    23521 tgagaagcac cgctcagggc ggtataaatc aggcggtcca ttacattgcc tggaacagcg
    23581 cgtcatcgag ctgcgtgctg gtggcgataa ctttggtgtt ggcctgatag ttacgctggg
    23641 cggtcatcag gttgaccagt tcgctggtga tatcgacgtt agagctttcg agcacgcccg
    23701 acgacagcgt accgcaggtg ccggagccgg gaacgccaat cagcggggtg ccggattcgc
    23761 ccgtttgtac ccatgcggtg ccgctcactg cctccaggcc gttttcattc gggaaagtcg
    23821 ccagcaccac ctgcccctgc aacatgcgct cgccgttgct gtaggtggcg taaactttgc
    23881 catcgtcatc aacctgaaca ccgttttgcg tggcggaggc gtagccgttg gcggcgttgg
    23941 tggtgaccga aaattcagag ccgtattgcg tacaggtgga gtaatcgacg gttaagtcga
    24001 cgggcgcggc ggcgtcggtt tgaaattcaa tggtctgcgg cgtggttgga gaggtcagct
    24061 tcccggtgtt cggatcgaag gttaacgtcg tcgccggaac gccggtctgc tgctgaccgt
    24121 cgaaggtgta ctgcacttcc caggtgttgt cgctggtttt ggtgaagtac tggcataccg
    24181 aatgttcgtt gcctaatgag tcatacaccg tggtggtgta gctgtcggtg tacgaactgc
    24241 tgttagtcgc gtcgaacgga acggtggtgc gatcgatagc ttcatcgctg gcgttgaagt
    24301 tagcggtaaa ggtcaggctg ctgctggcct gcgccgggat attgccggtt ttgatctgga
    24361 tatcggtgac cgtaccggtt tgcagagtgc cagtgtcatc gaccggatag ccctgcaaat
    24421 aagcgcccga ggcgttgacg atatagccgt ttttgtcggt ttcaaacgat ccggcgcggg
    24481 tataggagat attgcctgcg ctgtcacaca tgacaaaaaa tccgtcgtcg ttaatcgcta
    24541 aatcgagcgc attgccggtg gagaccagcg aaccgccacg cgaaatactt tgcgcggtgc
    24601 ccgccacgct gacgcccatc gcctggcttc cggcatacat ggcggaaaac tgggtggtca
    24661 tcgacttata gcccaccgtt ccggcgttag cgatgttgtt actgatcccg tccagctgtt
    24721 cgttaacggc attcagcccc gttgcggcaa tttcataact cattgtgtct tcctgttaat
    24781 tgattcttac gccacatcac tgtcggcatc gggtacgcca aactggctaa tcatggtgaa
    24841 cggcacttcg cccacgcctg caacgttaag caccggggaa ctgccgtcga gcgggatgcg
    24901 cacatcttcc acttcgccgg agacttcaat cggcacctct tcttccccgg tattggtcac
    24961 caccgagacg tcgtaatcgc ccggcgggat gccgtaatcc gcgggattca ccgagaaatc
    25021 aacctggcct ggtccgacgg acgaactgcc ttctgggatc agcgagacgg tgtagtcatc
    25081 gcctgcggca tcggtaaagt ggagatcggc ggtggtacag gcgtcgtcca gcgtagcgcg
    25141 accgttaatg gtctggtcgc tcacctgcaa ggcggtggtc tgcaccataa tgtcgtcgcc
    25201 caccaggtta ccgagcgcca tcacctgaat gttgctcatc agcaccgcgt tggtgttggc
    25261 ctgcacgctc atctcttcca tcatcgccac ctgcgccatc gacgacagct ggtcgatgta
    25321 ttcggtagcg tcggtcgggt cggtcgggtc ctggttttgg atctcggcga caaacagcgt
    25381 caggaaggta tcgacaggcg agctatcgct gctgctttgc gtgtcgctgg ccgccacatt
    25441 attgctttcc gccgtggcgg tcggctgcgg cgattgcgca ttcagcgcca gggtattcat
    25501 caggcttctc ccagtttcag caaactttgc tgcatacttt tcacgttgtt gagcacatcg
    25561 acgttggttt cgaaatcgcg cgaggccgac atcatgtccg ccatctgctc caccacgttg
    25621 acgtcgggat accagacgtc accgttggcg tcggcgagcg gagaatgcgg ttcatagcgt
    25681 ttcaccgccc cgccggtttg cagcacgtcc tgtacctgca cgctggcacc gtcaatggca
    25741 tgacgatgtg tacccgccag caggctgtgg tgatacaccg cggcaaacac cgggctgcgc
    25801 gctttatacg cctgcgcctc gctgctggcg ggcgcattgg cattcgccag gttactggca
    25861 acggtattca gacgtagcgt ttgcgccgtc atcgccgagc cggaaatctg atagatatca
    25921 gtaaatgaca tgcttatttc ccttcaatgg cctctctgat gccgctgatt tgcagattca
    25981 gaaaggtcaa actgctttga taatccatac tattttgtga aaaccgcgcc tgctcgctgt
    26041 tcagttccac ggtgttgcca tcttccgacg gctgcatcgg cacgcggtac tggacatcca
    26101 ccgccgcgtt atttgcccgt tgcatctccc tggcaaaatc aatatcttta gccttgaaat
    26161 ttggcgtatc gacgttcgcc agattcgcag tcagtagctc ggtcctctca agacgtaact
    26221 tcactgcctg cggatgtact cccaatgcct gctgaaaact gatccccatt tcctgcatca
    26281 ctcctgcttc aatccgcata cgctaagcaa tcgccatgcc attttttaag tcactgatga
    26341 gaaagggttt atgagttttc aattttgcag gcggaagttt tacttcctcc tggcgctggc
    26401 cttaccgggc tacgccgccg tccatcccgt gcaacacagc gcgcgggaac aagtcaacgc
    26461 ccaggtgctg aatgccgcga gccaaaaaat tgaatcgctg gcgcagcagc ggcagtggca
    26521 tgactatcgc tacaccttta agatctacat tccctcgcag attgcgaccg ccgcgccctg
    26581 tactaaaaca cccggcgtca cgctgacttc gcctgcggaa atcgccctca accggatgaa
    26641 cttcaccgtt agttgcccgc aaagctggca gatgaacgtg gcggtgcgcc cggatgtact
    26701 ggtgccggtg gtaatgacga agtcactggt tgcccgcgat atgccgctca ccgcgaatga
    26761 tgtggagctt aagccctaca acgtcagcgc ccagcgccgc gatgtgttga tggagcttga
    26821 tgacgcgatt ggtttaagca gtaagcacgc attgcaacca ggcagaccca tcaccaaaga
    26881 agaactggtg tctccggtgc tggtcgggcg cgatcagccg gtgatgattg tttaccagag
    26941 cgcggggatc accgcctcga tgcccggcgt ggcgctgaaa aatggtcgta agggcgagat
    27001 ggtgaaagtg cgcaatgcca gcagccagcg ggtcatcagc gcgatggtgg cggaaagcgg
    27061 cgtggtgaca acggtaagcg cggagtaatt tgcgcaggcg attaaagttt tgccgcctgt
    27121 taaccgcttt accgcagtaa cccacccaaa taccgagacg aggttgcgat gaaaatcacc
    27181 ccgactatgc ctggcaatcg cccgacagcc acaacggcag gctccagcca gccgcgcaaa
    27241 accacggtga ccaccaccac gacgctgagc gccgacgaca ttactcaggc gggattgcag
    27301 tctgcccagc aaacgctgaa cgaccagcaa gagagcgata tcgacagcaa caaagtggcg
    27361 caagtgcagg cgatgctggc gtccggcagc ccgcaagtgg acactgaaca gttagccagc
    27421 gacatgctga gttttttcca gaactaagag gccgatgaag tgagtatccg gttacaacag
    27481 gtgaaggctt tgttgcaggg cattcgtgac gatgacgcgc tgtatgattc cctgcgcgaa
    27541 cttctccagc gtcagcggat ctgcatgatt cgccgcgcca gcgaagagtt gctggcggta
    27601 aacgatgaga taacccatca ctatgagcag ttgcatggac atagccatca gcggcatagc
    27661 ctgctgaaaa tgctcggtgt tagcgtaaat cgtgacggtc tggcgcaggt tttcacctgg
    27721 cttccggctg ttcaaaaagc ggctgcacaa cagttgtggc aacgccttga gcaaaaagcg
    27781 gaacgctgca aagcctacaa cgacaaaaac ggcgagctgt tgattcgcca gtatgaattt
    27841 attcagtcgt ttttaggcag cgaagcagat tttctctatc aggaataacg cgaatacctt
    27901 ccccatagcg gaaatggatt ttccgcctga tttccaatcc cttgttttta attacaaaaa
    27961 tccccggcac gcttattgca tatcctgcac agcattaaag actggaagcc acctatgagt
    28021 tttattgaat gttacgaacc ggaatacgtg cgcaaatttt tagcgcgtcc ccccggttcg
    28081 cctttatacg tccgcaaggt gtggaaaaac gagattcgcc tgagcctgtc attgaccgat
    28141 attgccagct gcgaagcggt gctcaacgat gtcagggcct ttgatttaca gctcacctac
    28201 cgccctgtgg aagataattc cgcagagctt tccgcgcgcg atgcgatagt aaaccaggtg
    28261 attttgagta ccctcacgct ccgtgattta accccggagc tttccctgta tgctgtcggg
    28321 attttgctct cctgcgccag caaaatgccg ggcagagacg gagacatcct ggcccgtttg
    28381 acgacgctgc cacaggcgct ggccgatcat gcgaaaaaag gtacgctaca ggcacagtac
    28441 gcccagctcc cgcccgtgcc acagctggcg cgccagttga tgacgctgct cggcggttgt
    28501 gcgtttgact ggtcgatttt gccggaaagc ccgcgcaaag cctcgctgcc gttgcaggtt
    28561 actttactga cgctgcatga cgccaacagc gaagccctgt tacaacagca gcttcagacg
    28621 cagtggcaaa ccacctggca acagcatttt gccaccgcgc cctggatgat gcgtaactgg
    28681 ctgatttacc gcgtttacca cgatatgatt gggcagactg acggggcgga ttatttcccg
    28741 ctggtgtgcg atttttatct cctccgcacg ttaatcagtt tatggacgct ggatggttct
    28801 ccgctgcggc aggaggatat tttcgccctc tttgccatgt ttgaacgctg gcgagccagt
    28861 gaaaatgcgc tgttggttcg ccagcaaatt cagtcgttat gtgccgccga cccgctgctt
    28921 tcagcgtttt cgctgctaac gtgagtcgct ctgcgcgcgc tttgcccctc accctaaccc
    28981 tctccccaaa ggggcgaggg gaccgatcga gcacggtgtt aaaatcggcg ctgattttct
    29041 ccctctccct gtgggagagg gtcggggtga gggcatcagc gcgcacgttc atcacattca
    29101 cacattaact cacccaaaca ccatgccaac accaaccatc atcaaaacac tcgccaccca
    29161 ccgcgaaaaa ccgtttgtca gcgtcgtcac gccaacctgg aaccgcggcg cattcctgcc
    29221 gtacctgctc tatatgtacc gctatcagga ttacccggca gaccgccgcg aactcatcat
    29281 cctcgacgat tccccacaaa gccatcaaca catcatcgac cgcctgacca atggcacgcc
    29341 ggaagccttt aacatccgct acatccatca cccggaaaaa ctgccgctgg gcaaaaaacg
    29401 caacatgctc aacgaactgg cgcgcggcga atacatcctc tgcatggatg atgacgacta
    29461 ctatccggca gataaaatct cttacaccat cgctatgatg caaaaacacc gggcgctcat
    29521 ctccggttcc gaccagatcc ccatctggta cagccatatc aaccgtatct ttcaaagccg
    29581 cagctttggc ccacataata tcctcaacgg caccttttgc tatcaccgta actacctgaa
    29641 aaagcaccgt tacgacgatg attgcaattt aggcgaagag aaaagtttca ccgacaattt
    29701 cagcgttaac ccgctgcaac tgcccggcga gcgcacgatt ttgtgtatct cgcatagcca
    29761 caacaccttt gataaagact ttattatcgg ggccagcacg ccggtaaaca cggcgcttac
    29821 ggatatcgtc cgcgatccgc tgctgcgcaa tgcttatctg agtctgcata acgccactca
    29881 tcaccaggcg atcaaccatc aggcgatcga tcaggtggtc gtcgtgaatc tggataaacg
    29941 cccggatcgc ttacaacaga tccgtgaaga actggcattg ctgcatattc cacccgagaa
    30001 gatcacccgc cttgccgcca gtgaagatga aaacggtcaa cggggccgcc gtcagtcgca
    30061 cttgcaggcg ctgcgtctgg cgcaacagca cggctggcaa aactatctgt tactggaaga
    30121 tgacgcggtt attctcaagc aggagaaaca tattcaggta ctgaacacgc tgctggcgag
    30181 cctggcgaaa attccctggc aggtgatgat cctcggcggc gaaatttcgc aaggaacgat
    30241 gctaaaaagt ttgcctgggc tggtgcacgc gcgggactgc cgcaaagtgt gcgcttatct
    30301 ggtgaacagc cgctactacc cgcaactggc gcagcagatg agcaacgatg agcactcgct
    30361 ggaagactgc tggcaaccgc tgctacgcgc cgacaaatgg ctggcctgtt acccgagcct
    30421 ttgttatcag cgaccgggct ttagcgatat ccagaaaaaa acgactgata atattgctcg
    30481 ctatttcaat aagttacccg taaccaccaa gccatccgcg ttacccatcg ccgacaccat
    30541 cggctttttt atggaaacct cattccacta cgcgctgtac cgcccaatca tcaccgccct
    30601 acaggcgcaa gggctgagct gcacgctggt gatcaacgac cgcgtgttta aaccgtttct
    30661 ggatgagatg ctggaaacac tgaaaaacat cgacgatccg cagcttaaag gtatgcgtct
    30721 gtcagaaatg cagacacatg gtcagcgggt gaaatgcctg gtcagcccct accacacgcc
    30781 tgcgctcaat ggcctggcgg cggtgaacat ccgcgctatg tacggcttag ccaaagagac
    30841 gtggaaccac gccgactgga atcgttttta ccaaagcatt ttgtgttata gccactacag
    30901 tcagcaggcg cttgcgcatt tcggcagcgc aaaagtggtc ggcaacccgc gcttcgacgc
    30961 ctggcataac ggcacgttcg atcgcgcgtt gccggaaaac atccaatcag attaccgtaa
    31021 acctacggtg ctctatgccc caacgtttgg cgcattaagc tccctgcccc actgggctga
    31081 aaaattgggc cgcttgagcg gtgatgtgaa cctgatttgc aaactgcacc atggcacctg
    31141 ctcacgcccg gaagaagcag catcgctggc cctggtgcgc aggcatttaa aacagcgcac
    31201 cgactccgcc cgccacacgc tggcgctgct ggcaaaagcc gattacgtgc tgaccgataa
    31261 cagcgggttt attttcgatg ctatccacgt tgataagcgg gtgatcctgc tcgactttcc
    31321 gggcatgaat gatttgctcg acggtgagaa aagttattcc acggcagaga gcgccgacca
    31381 gcgaatccgc gaaatattgc cggttgctca tgatgttgca gaactgcgtt atttgttgtc
    31441 agaggcgttt gactgggggt cagtgcaggc gcggcttacg gagattcgcc atcactattg
    31501 cgatgcgttt atggatggca aggcgggaga acgggcggcg attgtgattg tggaggcgct
    31561 ggggtaaacc gcgcaactgc ctgatgcgct tctcttatca ggcctacgga tcgatcacgt
    31621 cctgtaggcc ggataaggcg tttacgccgc atccggcaag aaaatacatc tctgttttag
    31681 tactttataa aagccctcta atcacctcaa taatccccgt cgtcgacacc gacggcgtgc
    31741 gggggaaata gaccacctca caaatatacg caaagctgtc aaacttccct gcccagtcat
    31801 cccccatcac taaaatatcc gcactgtacc cgcgcaaata ctccgctttc tgctctagcg
    31861 attcttccag aaatacgcca tccacgcacg ccagcccggc gatgatcgcc atgcggtcat
    31921 cctgatggta aaccggtgcc ctgccctttt tggcgatgtt cagggcgtcg gacgatacgc
    31981 cgaccagcag ccgctcgccc aatgcgcggg cgcgttgtaa aaggcgcaga tgaccgacat
    32041 gaaacacatc gaaggtgccg aaggtgatga ccgttttcat ggctactccg ctaatttgtg
    32101 ccgcagccag cactgcacgg cgatgtcgtc caccgctttt tgcgctttcg ccgtggcgct
    32161 ttcttgttgc tcgcggcgtt tgtcgtccag cgtcagggcg actactttct cttcgcacgc
    32221 cgccgccgtg aggtttttct gcattcgcgt ggctttgata ttggccagcg ttttttcctg
    32281 ctcctgccag gcgattacct tgcgcagggt gcctttgtag ccgctgacgt ttttcagcga
    32341 ttccaccgac accgccgcac ccgcagatgt tttctcgaca aggtagccta acgccttgat
    32401 attgttgtca taacccgcgc aaatctgttt ttgctttgcc agctcgacgg ttttgtcttt
    32461 gaccgattta tcccgcaggc gctgcaactg cgtcagggtg tcgatgattt gtcgcatagt
    32521 tactgttctg cctttttgac accaggaaag agaatttgca ggcggctttg caccagttcg
    32581 agggaggcgg gttcgcgcat ctcctggcgc aaaaatcgct cgatggccgg gaacattttt
    32641 accgctttgt cgacgctggc gtcggcacct gccacataac cgccgagcgg gatcagaggt
    32701 ttgatctcca tataggcggc atagttttgc tttaacgcgc gggcggattg ctgatgttcc
    32761 aggctggtga cctggttcat gcagcggctg atcgactggc cgatatcaat ggccggatag
    32821 tgccccgctt ccgccagttt gcgggttaat acgatatgcc cgtccagcac cgcgcgcgcg
    32881 cagtcgacaa tcggatcttg ctggtcgtca ccttccgcca gcacggtgta gatggcggtc
    32941 atcgatcccg cgctttcgct gttgcctgcg ctttcgacca gtttggggat catgccgaat
    33001 gccgatggcg gataaccttt ggtggcgggc ggttcgccga gcgagagggc gatttcccgc
    33061 tgcgccatcg cgtagcgggt cagggaatcc accagcagta agacgtgatg cccgcggtcg
    33121 cgaaaccagg cggcgatgga gtggcacagt tcggtggctt tcagacgcat cagcggcgat
    33181 tcatcggcag gcgcgacgac gacgatagac tttgccagcc cgtccgcgcc cagcgagtgg
    33241 tcgataaact ctttcacttc gcgcccgcgt tccccaatca gcccaaccac cacgatatcg
    33301 gctttggtct ggcgggtgat catccccagc agcacgcttt tcccgacgcc gctgcccgcc
    33361 attagtccga cgcgctggcc tttgccgatg gtcagcaggc cgttgatcgc tttcacgccc
    33421 acgtccagcg gttcgtctac cgaacgacga gttaagggat taaccgaggg cggcaacggc
    33481 ggcagaaggt cgttgccgtt caggcggcct ttgccatcca gcggttcgcc gaggccattc
    33541 accacgcggc ctaaccagct ttcgcctatc aggatgtcgt gggctttttc ttccgggaag
    33601 acgcgtgcgc ctgccatcag gccgaccggc tgtttaaacg gcattaaata ggtgatgtcg
    33661 cgattaaagc ccacggcctg cgcgtcgatc agcgtgtgat tggcactttc gatgcggcat
    33721 agctgcccgg tcattagcgg gcagccgaca ctctccagca ggatgccgtt aaggcgcacc
    33781 agccgcccgg cgacgcgggc cagcgggatc gactccagcg agcgcagggc gttatcgaac
    33841 gcagaaaaat cactcaccct gcgactccgg cgtgagtgtt tctttcagcg cggtcatgca
    33901 ttgttcaagg cgatgctcgc agccgatatc cagttcggat ttgtcggtga tcacccggca
    33961 ttcgcccggt ggcaaatccg gcgaaggcgt cagcccccat tcgctgacct tttccggcac
    34021 cgcgtcgcgc aggcggttaa actcttccgt gctcagcatt acttgcaggg tttccggcat
    34081 cgccgggaag gccgccagcg cctcttccac cagcgccagc aactgcgtgg gttgcagcgc
    34141 cagttcacaa cggatcacct ggcgggtgac tttttccacc agttgcagca aatcttcccg
    34201 ctgtttacgt tcgatatgag cgaggaagtc gttcacttta ccggtgatgg cttccagcgg
    34261 ctgcgccgcc tcaacaaact gtttgcgccc ttcctgctgc ccggcgaggc tgccttcggt
    34321 gtaaccctgg cgtcgcccct cggcaaaacc ttgctgatga ccttcgctaa agccctgctc
    34381 ctggccttcg gtcattcctt gagcaaaccc tttttgcagc ccttcctgaa atccgtccat
    34441 caactggcgc tggtaatcgg cggcgctaat actggtagtg agcggttctc cggtcaccgc
    34501 acgctggcgc ggcgggaaac ggtgcaggcg atagcgcccg cgcagtgttt caatggtcat
    34561 ggttactccg ccgtctgttc cgcaaagagc tgtagctgaa tttcgcccgc ctcttccagt
    34621 tcacgggcga tgcccatgat ttcgcggcgg atctgttcga tacggctgac cggcaccggc
    34681 cccagacgcg cggtgatggc ttccagttgc tgcgcctgcc gttttggcat taccgcgtaa
    34741 atcgaacggc gcagcagggc ttcggtgcct ttcagcgcca ccgcccagtc ttccatcggc
    34801 acttcgtcaa gcagacgtcg acgcacctct tcgttctggc ggctgaggat aaagaagtcg
    34861 tacatctcat cctgaagctg ctccagcacc tcttcgtcgc gctcgcgcat ctggtcgagg
    34921 atcacctgct ggttgccctg gaagcggttg acgatatccg ccgcctgttt gatgcctttc
    34981 acttttgagc cgtgttccga aagcaccgac aggccgcgtt cgatcagcct gtccagttca
    35041 tccaccacgt cgcggttgac gtcgttgagt ttcgccaccc ggtagagaat ttcgttttgc
    35101 accgattcgt tgagatacga gagcaccgct gccgagattt ccggcgtcag aaacgcgagg
    35161 aacaccgcct gtaattgcag atgttcttcg gagatcagca tcgccagctg gcgtggttcc
    35221 acccattgca ggcgcgccat ccgcgagcgg atctcatcgc cgtagatgcc gttgatcaca
    35281 ctgctggcga tctcggtgcc gagggctttg ttgagaatac cctgcaacat ggatcgcgat
    35341 gcgccgttga tcccgctctg ttcgcggaac tcatcaaaga agttattgat cactttgcgc
    35401 gccatgctgg ttttcacgcc ggaaagccgt gccatgtttt cgctcaggcg caccacttct
    35461 tcgcggctga gtttttgcat caccgtcgcc gccgcctctt cgcccagaca cagtagcagg
    35521 atcgccgcct gttccaggta gctgttgctc ttatttgttg tcaattcgct cattgctggt
    35581 gatccattgt ctgagaactt cggctacgcg atcggtgtcg ctcatggcca gtttttgcag
    35641 gaactcgagt tttacttcca ggccggaact ctgagacggc aggctgtcat cgccgggcag
    35701 cgacggcaga tcgacgtttt tgcgctcctc ttccggcatg gcaatccacg gttcgctgac
    35761 ggcttccgat tctggtgtga taatcggtgc acgtaccgcc gtcagccgtt tcattaccgg
    35821 gcgcaccacg aagaacagca acaacagcgc cagcaggccg cagccaatca ggcgcaccca
    35881 ggccagcacg ctgtcatctt tccacagcgg aataattggt tcgaccggca ccgcctgcgg
    35941 cacaaagtta agcagtgaaa gcgtcaggtt atccccacgt tgcgcgtcaa taccggcggc
    36001 attgttcagc aacgcggtaa gctgcgcggt ctgctccggc ttccagtttt tcagcgccgg
    36061 agcgttttga ttgagcacca ccgccacatt gaggcgtttc accgcaaagc cgggatgctg
    36121 aatatgctgg acgctacggt cgtaagagta atcgcgcttg ctttcgctgt gtttggataa
    36181 cgcttcaggc gagcgatttt cttccgtacc gttagtcatt tgattcgccg caaccggcgg
    36241 acggttgctg agagagccgg gcacacccat cgccacctga ttggtgtcgc tgtccagcac
    36301 gctctcttcg cggtttattt tcggcgcgtc gccgtagtgt tcctgagttt cgtcgatggt
    36361 gctgaggtcg agatccggca tcacgctgac acggtaattc ccgctgccga ccagcgaatc
    36421 cagcacgttc gccacgctgg cgtgggtttt gtcctgaatg tctttgagga tctgatcgcg
    36481 tttacgggta gcggcagaga ccgcctcgcc cgcgccgatg ccgtcggtca gcagatttcc
    36541 cgcctgatca accacgctaa ctttgctggc gtgcagcccc ggaatgctgc cggaaaccag
    36601 atgcacaatg gcgttcacct ggtccatatt cagcttcgcg ccgtagtgca gacgcaccac
    36661 caccgaggcg ctgttttgcg gctcatcgct gaccacaaac gagctttctt cgttgagcgc
    36721 cagatgcacc cgcgcgctct ccaccgcgtc cagcgacata atgctctgcg ccagctcccc
    36781 ttcgaggctg cgtttatagc gcacgttctg cacgaactgg ctgctgccaa gcacttcgtc
    36841 tttgtccatc agctcgtagc cgctgggcaa aatcgcctgc acgcctttgg cggcaagggt
    36901 catgcgcgtt ttcgacagcg cctcttccgg caccagtatc tgcccgcttt gcgggtcaat
    36961 gcggtaggtg agcttttcgc cgtccagcac ggtgacaatc tgcgacacgg gcagattttc
    37021 ctggcgtccg tagagcgaga cgtagccgcg attgccggtc cacagcacgc taacgataat
    37081 cgccgtcgcc gccacggcaa gccccacacc cgccatcagc gcccagcgtt tgttatctgc
    37141 gagacgcagt cgaaacgccg gaaacgccag ggttaacttc tttatttgtg cattcatagg
    37201 gttagatcga catactcatc agatcgttaa atccggtggc gattttgttg cgcacctgca
    37261 ccagcgcgga gaaagagagc gacgcctgct ggctggcgat catcgcacct gccagatcat
    37321 cgcttttgcc catgtcgata gccgtttgct gctgctccgc cacctgctgg aactggtcaa
    37381 cgtgaccgag cgcgccctgc ataatgcggt gaaacgaaac gtccgggtcg gtggaactga
    37441 aggtcatggc gggcagcacc ggcgcctggg cgttggcctg catccgctgc acgtcctgca
    37501 tgatctgcgc ctgcatggtg ctgttgattg tggtaatgct catttttaac ttccgggtca
    37561 ggagaaaact tcgataccgt gcttgcgcat ggaggccagt cgatagcgca gtgcgcgcgg
    37621 ggtaatacct aacaggtcgg cgattttgct gcggttgccc tgatatttac gcatcaggtc
    37681 agcgatatat tgatactggg cgcttcgtcc gtgctggcct aaatcgctgg tttcggcaat
    37741 gtgtacggcg ggctgagcag gccattgcag ttcgctgtgg tcggcaatgt cattttccgg
    37801 caggcctaag gcgtccgggt aaatcacgcc gtcgcgattt aaaatcattc cccgctgaat
    37861 ggcgttctcc agctggcgca cattgccggg ccagcggtaa ttcagcatcg cacggcgggt
    37921 agattctgaa agggtgatat ttttcaccag caccgtggag tattttttta taaatgactc
    37981 tgccagcgga ataatatcgt tcagacgttc acgcagcggc ggcatggtaa taggaataac
    38041 cgccagacga taatagagat cttcacgaaa acgcccggcg gcgacttcct gctcaaggtt
    38101 tttattggtg caggcaatca ggcgaaaatt gagtttaatt tgtcggttgc tgcctaatcg
    38161 ctcaacctgt tgttcctgca atacgcgtaa tattttggcc tgtaacgcca gcggcatatc
    38221 gccaatttca tcgagtaata aggtgccgtt attcgccagt tccatttttc ctggcacact
    38281 ggcaattgcg ccggtaaatg cgcctttgtc gtagccaaat aaggttgctt ccagcatgtt
    38341 ttccgggatc gcagcgcagt tcacgccgat atagggcgcg ttttcatttt caccaaaggc
    38401 gactgtgtga atatatttcg ccacgcactc tttgcccgcg ccggtttcgc cctgaatgag
    38461 caccggcacg ttaaatgcgg cgacacgctt tgccagagta aaagcgttaa tgctggaagc
    38521 ggcagttgca ataagttcag acatagggtg aacgatataa gtagcgacaa taatcaggat
    38581 gttctgaagc gtatcaccgg cgggattaac aagttcgaat taaacgataa taaaaacatt
    38641 atttgtttat aaacaatgat ttatccatgt aggccggata aggcgtttac gccgcatccg
    38701 gcaattgtgc accggtgcct gatgcgatgc ggcgcgtctt atcaggccta cggttcgtgc
    38761 aatgtgttat gacgatgcct gatatctcgc cctttaataa tttaattatt ttttcaccct
    38821 attaataatt tgcccctttc acgccccctc tatttaatac ctcaccttgt gtcactgccg
    38881 gatttgcgtg cacaaaatcg cccataccat tacagcagag aaagtaagcc atgctgaagt
    38941 acagtaaaac gccaggcatc tttaaattag aaggtaatcg gctcggacgc ccctaccatc
    39001 gcctgccgac cctgtttaca gggaattttg acgtgattga ttcgcatctg ggcagctact
    39061 ttctcaaaaa gcatcgcagc aatatcaccc taaaaaagat tggctgcgaa atggatatta
    39121 ttaataaaaa cgctgaactg atggtttccc aggtcgggca tctggcgttt gatattgatc
    39181 gctctttgct gttaatgctg ctgggcaatt tttatggtct ggagtcctct cttgaagagg
    39241 cgaaagcgca taacgggctg ccgaccaaaa ccgaaacgcg gttaaaaaac cgtctggcgc
    39301 tggatatctg cacgctgatt tttaatctgc aaacctccgg tattgcgctg aaattaaagc
    39361 tggacagcag cacggtaatt acccactggg cgtatcaatt aacctttacc cttgcgggcg
    39421 atgaagagag ctgttttcgt attttgctcg acgacgccca caccgatttt attttaaatc
    39481 tgatccgcca cagcgagcac agtaaaccgc agcaggtggc gaaatcggtc aataaaccgg
    39541 ccctgattaa agagatcatc cgctccctgc cgctgaccct gaacgtcaaa atcgccgagc
    39601 tatcgatgaa tgtcgcggat ctcacgcaga taaaagccgg ggacatttta cccatctcgc
    39661 tgggagaaac gttcccggtg gcgattgggc aatctgaatt atttagcgcc ctgattgtgg
    39721 aagacaaaga caaactgttt ttgtcggagc tggcgggcaa aaatgagaat agccatgagt
    39781 aagcaagaag atattttagc cggggatttt ggcctcaccg atgaagttgc gcctgtcgca
    39841 aaatccgccg acgctgaaac gctggtcacc cgcctggaag accgtttttc cgactcgatg
    39901 acgctgctaa aacgcattcc ggtgacctta acgctggaag tgtcatcagt agaaataatg
    39961 ttagccgacc tccttaacat cgacgacgac acggtgattg agctggataa actcgccggg
    40021 gagccgctgg atatcaaagt gaacaatatc ctgctgggca aagcggaagt ggtggtggtc
    40081 aacgagaagt acggcctgcg cgtgctggaa tttaacaccc gcgatatcaa cgacctggca
    40141 ccatgaaacg ccgcactcaa ctgacgctgg gccttgggct gctggcgctt gcgccggtgg
    40201 cgttggccca gggcggcgat attgcgctgc ttaacgtggt cacccacggc aacactcagg
    40261 agtacagcgt caaaatccag gtgctgattc tgatgaccct cgtcggtttg ctgcctacga
    40321 tggtgctgat gatgacctgc tttacgcgct ttattatcgt gctgtcgctg ctgcgccagg
    40381 cgctagggct acaacaaacg ccgccaaacc gcattctgat tggcattgcg ctctccctca
    40441 ccatgctggt gatgcgcccc atctggctca atatttacga ccacgcagta gtgccgtttg
    40501 aaaatgatca gattaccctg accgatgcgt taagcaccgc cgcgacgcca ctcaaacgct
    40561 ttatgctggc gcagactgac aaaaaagcga tggcgcaaat catgactatc ggcggcgcga
    40621 agggccatgc cgccgaccag gatctctcga ttgtggtgcc cgcctatgtg ttgagcgagc
    40681 tgaaaaccgc cttccagatt ggctttatga tttacatccc gtttctggtt atcgacctga
    40741 ttgtcgccag cgtactgatg gcgatgggga tgatgatgct gtcgccgctg atcgtttcgc
    40801 taccctttaa gctgatgctg tttgtgctga tcgacggctg gtcgctgacc atcggtacac
    40861 tcaccaccag cattcgcggg ctggggctgg gctgatgatg ctgaccatag acgtagccgc
    40921 cgacattgtc gccagcggca taaaagtggt gattttgctg gtttcggtgc tggtagtgcc
    40981 gagcctgctg gtcggcctgc tggtgagcgt gttccaggcg gtaacgcaga ttaacgaaca
    41041 gaccctaagc tttttgccga ggctgattgt cacgctggtg gtgctgggcg tgtgcggtaa
    41101 atggatgatt atccagttgc atgatctctg cattcacctg tttacccagg ccgcgttgct
    41161 ggttcattga gatgcgcaca agcgacgtta cgcaactgat ggatctggcg ctggggctgt
    41221 ggtttccctt tgtgcgcatt atggcgtttc tgcgctatgt gcctgtgctg gataacagcg
    41281 ccctgaccgt gcgcgtgcgg attattttgt cgctggcgct ggcaatcatc atcacgccgt
    41341 tgatccccca tcctgttccc cacgacctga tatcgctcaa cagcctgatc ctgacggtgg
    41401 agcaaatttt atggggcatg ttattcgggc tgatgtttca gtttttgttt ctggcgctac
    41461 agctggccgg gcaaattctc tcgttcaata tggggatgag catggcggtg atgaacgacc
    41521 cgagcagtgg cgcatcgacc accgtgctgg cggagctcat caacgtctac gcgatcctgc
    41581 tgttttttgc catggacggg catctgctgc tggtgagtgt gctgtataag ggctttacct
    41641 actggccgat tggcaacgcg ctgcatccgc aaaccctgcg caccatcgcg ctggccttta
    41701 gctgggtgct ggcgtccgca tcgctgctgg cgctgccaac cacttttatc atgctgattg
    41761 tgcagggctg ctttggcctg ctcaaccgca ttgcgccgcc gctgaacctt ttttcactcg
    41821 gttttcccat caacatgctg gcggggctgg tctgctttgc caccctgctc tacaacctgc
    41881 cggatcacta tctgcatctg gcgaattttg tgttgcagca actcgacgcg ctgaaaggtc
    41941 actatggcgg atagcagcag cgaagaaaaa acagagaaac cctcggcgca aaagctgcgt
    42001 aaagcgcgcg aagaggggca actgccgcgc tcgaaggata tggggctggc ggcgagcctg
    42061 tttgcggcat ttgtggtgat ctccagcagc ttcccgtggt atgccgattt tgtgcgcgag
    42121 agttttatca gcgtgcacca gtatgcacag gagattaata acccagaggt gatcggccag
    42181 tttctgcgcc atcacctgtt aattctcggc aagtttattc tcaccttgct gccgatgccc
    42241 gccgccgcgc tactctcctc actagtgccc ggcggctggc tgtttctgcc taaaaaaatc
    42301 ctcccggatt tcagcaaaat cagcccgtta aaaggcattg agcggctgtt ttccagtgaa
    42361 catctggcgg aaacgggaaa gatgacggtg aagtcggtgg tggtgctggt gatgctgtgg
    42421 gtaagcctgc gcaataactt tgcctccttt ctcggcctgc aggcgctacc gttcaaactg
    42481 gcgatgaatg agggtttatc gctctacgcc tgcgtgatgc gcaactttgt catcctgttt
    42541 atcttctttg cgttaatcga cgtgccgctg gcgaaagccc tgtttaccaa agggctgaag
    42601 atgaccaaac aggagctgaa agaagagtat aaaaaccagg aaggcaagcc ggaagtgaag
    42661 gcgcgcgtgc ggcgtctgca acggcagttg gcgatggggc aaattcgcaa agtggtgccg
    42721 aaagccaacg tggtgatcac caacccgacc cactacgccg tggcgttgca gtatgactcg
    42781 tcgcgcgccg ccgcgccgtt tgtggtggca aaaggcaccg atgaaatcgc cctctatatt
    42841 cgccaggtcg ccgctgagaa tcaggtggaa gtggtcgagt tcccgcgact ggcgcgctcg
    42901 gtttactaca ccacccaggt gaaccagcaa attccgtttc agctttaccg ggccatcgcc
    42961 cacgttctga cctacgtcct gcaaatgaaa cactggcgcg agggtgcaca gccgcgcccg
    43021 gccctgaaca gacatatttc cattcccaaa gaggtgctta aactggatgg cgaaaacaac
    43081 taaatcgttc ctcgcgctgc tgcgcggcgg caatcttggc gtgccgctgg tgatcctgtg
    43141 tattctggcg atggttattt tgccgctgcc gcctgcgctg ctggatattc tgttcacctt
    43201 caacattgtg ctggcggtga tggtgctgct ggtggcggtg tcggcgaaac gaccgctgga
    43261 attcagcctg ttcccgacca ttttgctgat caccacctta atgcgcctga cgctcaacgt
    43321 cgcctctacg cgcgtggtgc tgctgcacgg gcatctcggc gcggacgcgg cgggtaaggt
    43381 gattgagtcg tttggtcagg tggtgatcgg cggcaacttt gttgtcggct ttgtggtgtt
    43441 tatcatcctg atgatcatca actttattgt cgtcaccaaa ggggccgagc gtatttccga
    43501 ggtttcagcc cgctttaccc tggatgcgat gcccggtaag cagatggcga ttgacgccga
    43561 tcttaatgcc gggttgatca atcaggcgca ggcacaaacc cgacgtaaag atgttgccag
    43621 cgaggccgat ttctacggcg cgatggacgg ggcatcgaaa tttgtgcgcg gggatgctat
    43681 cgccgggatg atgattctgg cgatcaacct gatcggcggc gtctgtatcg ggatcttcaa
    43741 atacaacctg agcgccgacg ccgccttcca gcagtatgtg ctgatgacca tcggcgacgg
    43801 gctggtggcg caaatccctt ccctgctact ctccaccgcg gcggcgatta tcgtcacccg
    43861 cgtcagcgac aacggcgata tcgcccatga cgtgcgtcac cagttgctgg caagcccatc
    43921 ggtgctctac accgctaccg gaattatgtt cgtactggcg gtagtgcctg gaatgccaca
    43981 tctgccgttt ttgctgttca gcgccctgct tggctttacc ggctggcgga tgagcaaacg
    44041 cccgcaggcg gcggaagcgg aagagaaaag tctcgaaacg ctgacccgca ccatcaccga
    44101 aaccagcgag caacaggtca gttgggaaac cattccgctg atcgagccca tcagcttaag
    44161 cctcggctac aagctggtgg cgctggtgga caaagcccag ggcaacccgc tcacccagcg
    44221 tattcgcggc gtgcgacagg tgatatccga cggcaacggc gtgctgctgc cggagatccg
    44281 cattcgggaa aacttccgcc ttaagcccag ccagtacgct attttcatca acggcattaa
    44341 ggctgatgaa gcggatattc cggcggataa actgatggcg ctgccctcca gcgaaaccta
    44401 cggcgagata gacggcgtgc tggggaacga cccggcgtac gggatgccgg tgacctggat
    44461 ccagcctgcg cagaaagcga aggcgctgaa tatggggtat caggtgatcg acagcgccag
    44521 cgtgattgct acccatgtaa ataagattgt gcgcagctat attcctgatt tatttaacta
    44581 tgacgatatt acgcagttgc ataaccgttt ggcgtcgatg gcaccgcgcc tggcggagga
    44641 tttaagcgcg gtgctcaatt acagccagtt gctgaaagtg taccgggcgc tgctgaccga
    44701 aggcgtttcc ctgcgcgata tcgtcaccat cgccaccgtg ctggtcgcca gtagcgcggt
    44761 gaccaaagat catattctgc tggcagccga tgtgcgcctg gcgctgcggc gcagcattac
    44821 ccatccgttc gttcgcaagc aggagctgac ggtgtatacc ctgaataatg agctggaaaa
    44881 tctgctgact aacctagtga atcaggcgca acagggcggt aaagtgatgc tcgacagcgt
    44941 gccagtggac ccgaatatgc tcaaccagtt ccagagtacg atgccacagg tgaaagagca
    45001 gatgaaagcg gcggggaaag acccggtgct gctggtgcca ccgcagctgc gccctttgct
    45061 ggcgcgttat gcaaggttgt ttgcgccggg gctgcatgtg ctgtcgtata acgaagtgcc
    45121 ggatgagctg gagttgaaga ttatgggggc gttgagttga tgggggattc ttttccatgc
    45181 tttgcagcac ctgacaccat taattgacgt cacactgcaa agagactacg atgacaccat
    45241 acaatgacgt catggattga tagtatgatt aaggtcagcg cgctaggttc tcgtcataga
    45301 aaaacattcg acgatgtaat gacggagccg ccaaaatctg gaattaaatg ggatgatgtt
    45361 gttgcactca ttaaagctgt gggtggaaca ataaaaaata acaacggctc cagacgaaaa
    45421 tttcagattg gaacgaccaa attcagcaca catgagccac atccaaaaaa tgtgatggat
    45481 aaaggcgcag tagcaggtct taaggagtgg tttgttaact gtgtaggggt agattatgaa
    45541 taaagcatcg aacattatca caattaaagg tcaaccagcc gcagtaacat ttgaagccga
    45601 tatcaacgca tttcgcggca agttcctcaa tgtaaacggt tattgtgact ttgtagccac
    45661 aagtattgag gcattatatc gtgaaggcga aagcgcgctg gatgagtgga tggcggattg
    45721 tgaagaggac ggtattgccc cttttcgtga agaagaagag caaaaacgcc tgacgcttcg
    45781 tgttcctcat cacattgatt cgcggctgac catcgtcgcc agacaacatt ccatttcaaa
    45841 aaaccagctc attgttgatg tcttagaacg tgagttttca gcccagatgt aaacaggtcg
    45901 gatgccgcgt ggcatccgac cttttgccag ttcgtcaata ttacccattt acaaaatcgt
    45961 ctcctccgtc aaaatccgcc gcgcgcctaa aaagtgatcc tgccagaaag gttcagctaa
    46021 ctgacttacc cgaatggttt ccccggtgcg cggtgactca ataaactgac cgtcgcccag
    46081 atacaccccc atatggtcgg caatctcgcg gctgtggata tggaaaaata gcaaatctcc
    46141 ccggcgcagg tcgttattcg ccacaatggt tgcccggcga tagtgataca tctcattagc
    46201 cgtgcgtggg agtttcgcct caaggatctt gttgtaggca taaaaaacta acccgctaca
    46261 gtcgaagcct tgatcgggcc gcgtaccgcc ccagacgtaa ggcttgccaa gctgttgctc
    46321 cagacggtga atcgccactt cggtgatgtt atgcagatgg tcgctgctta agaaatggtt
    46381 gttttccgcc agcgcctgcc agcgtctgtc actcgccttg agcggcgcag gaaaccattc
    46441 tggatgctgt tttatctgtt cgcggttgtg ctggcgtagc gcccttctgc tttcggcatt
    46501 gccttccaca atatagctgg cctgtttttt cagatgggtt tggtactgtt ttaataaacg
    46561 cgcacgattt tgcatccgct ggcgggcggc gtagctgaaa gtgatatgtg atgtggtggc
    46621 gctggcaaat gaagaaaacg gcaagcaaag caacagtaat cccgacaaca caaaagaggg
    46681 aatcgacggc aaggacatac tcaataatgg ctgatcatgc attgatgttg cagtgtatcc
    46741 cggcccgtac ctgaacaaac ttaataaacc attattataa aattaataaa tatatttcag
    46801 ttaattatgt aaaaacatcc gtttattaaa ttactcatgg attgtcgctg ttgctcattt
    46861 gagctacaat tcaagctgaa taaatataca gcacaggaga taccccaatg gctgctaacg
    46921 catttgttcg cgcccgaatc gatgaagatc tgaagaatca ggcggcggac gtactggccg
    46981 ggatggggct gaccatctct gacctggttc gcataaccct cacaaaggtc gcgcgtgaaa
    47041 aggcattgcc gtttgattta cgcgagccta atcaattaac cattcaatca atcaaaaaca
    47101 gcgaagctgg cgttgatgtt cataaggcca aagacgccga tgatttattt gataaattag
    47161 gaatttaaat caaagggata ttgaatactc gggacaattt tcaaaggatg taaaacttgc
    47221 acaaaagcgt cataaggata tgaataaatt gaaatatctt atgacgcttc ttatcaataa
    47281 tgctttaccg cttccagctg tttataaaga ccacccgctg caaggttcat ggaaaggtta
    47341 tcgcgatgct catgtcgaac cggactggat cctgatttac aaacttaccg ataaactttt
    47401 acgatttgag agaactggaa ctcacgcggc gctctttggg taaagcacgc tgccgatgaa
    47461 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
    47521 ccaaatccag actattccga tttgggatct attttgccgt cctggcgtgt gtaaccgttt
    47581 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
    47641 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
    47701 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
    47761 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
    47821 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
    47881 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
    47941 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
    48001 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
    48061 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
    48121 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
    48181 tgggagcagc tgaagccggg ctacgactac tttgagcaaa cccgtaagcc accgaccgtt
    48241 tctgtggtca atggccgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
    48301 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
    48361 cttttgccag gtttcattgc ccgtcagcgg ctgtgttgca atcaccgtga ccacatcatt
    48421 cggtgtggtc tgcgagctga agtcgatttc cacatcctga tccagcagcg ttgccacgcc
    48481 aaacggcgcg cggcgggtga tccagtgtaa attagtcgag caatacgcca tcacatagcg
    48541 cccgtcagaa agcagcatgt tgaaaacgcc cttctgccgc agttcatccg ccagtgaggc
    48601 gatatattta aacactgccg ccatgttgcc cggtgtgcgc gggtaacgct gcgttaattt
    48661 atgcaggagc cagcagaagg ctttttcgct atcggtttca ccgaccgggc ggaagttgcc
    48721 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
    48781 ccataactcg cgggtaaatg ggtgagtatt ttccagcgcc acctcgcccc gattagcctg
    48841 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
    48901 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
    48961 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
    49021 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
    49081 taacctccac aacagcggga gcgaatggcc cccggcgaag attgatttaa gaattttcag
    49141 gtcggatgcg gcgtaaacga attattcggc ctacggttcg ggtacagatt tgtaggcatg
    49201 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
    49261 ccttatccag cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
    49321 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
    49381 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
    49441 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
    49501 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga
    49561 aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag
    49621 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
    49681 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
    49741 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
    49801 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcctggtaca
    49861 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
    49921 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
    49981 cagcaacaat gtgagctttg ttgtaattat attgtaaaca cattgctaaa tgtttttaca
    50041 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
    50101 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
    50161 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagcctccct cggcgttgct
    50221 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
    50281 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gcggtttccg taaggtgatg
    50341 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
    50401 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
    50461 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
    50521 gatttccaga tcacccatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
    50581 gagcatcgtt tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga gttctcggct
    50641 tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga gcgggatcct ggcgattacc
    50701 gtcggcgtgc caaactcatt aggcccgggc gaactgttgc aacattacgg cactgacgag
    50761 cagaaagatc actatctgcc gcgtctggcg cgtggtcagg agatcccctg ctttgcactg
    50821 accagcccgg aagcgggttc cgatgcgggc gcgattccgg acaccgggat tgtctgcatg
    50881 ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa acgctacatt
    50941 acgctggcac cgattgcgac tgtgcttggg ctggcgttta aactctccga cccggaaaag
    51001 ctgctgggtg gcgcagaaga tttaggtatt acctgtgcgc tgatcccgac caccacgccg
    51061 ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtac cgttccagaa cggaccgacg
    51121 cgcggtaaag atgtcttcgt accgatcgat tacatcatcg gtgggccgaa aatggccggg
    51181 caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc
    51241 aactcaaccg gcggcgtgaa atcggtagcg ctggcaacgg gcgcgtatgc tcacattcgc
    51301 cgtcagttca aaatctctat cggtaagatg gaagggattg aagagccgct ggcgcgtatt
    51361 gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg cattatgctc
    51421 ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca ccgcgggcag
    51481 cagtcgatta ttgatgcgat ggatatcact ggtggcaaag gcattatgct cgggcagagc
    51541 aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga aggggccaac
    51601 attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca tccgtacgtg
    51661 ctggaagaga tggaagcagc gaagaacaat gacgtcaacg cgttcgataa actgttgttc
    51721 aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg cctgacgcgc
    51781 ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca gcacctgaac
    51841 cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggctgtgct gggcggcagc
    51901 ctgaaacgtc gtgagcgcat ctcggcacgt ctgggggata ttttaagcca gctttacctc
    51961 gcctctgccg ttctgaagcg ttatgacgac gaaggccgta atgaagccga cctgccgctg
    52021 gtgcactggg gcgtacaaga tgcgctgtat caggctgaac aggcgatgga tgatttactg
    52081 caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt cccgaccgga
    52141 cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat tttacaagtg
    52201 ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag cgagcataat
    52261 ccggttggct tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc aattcatcag
    52321 cggatctgta aagagctggg taaaaacctg ccgtttaccc gtctggatga actggcgcac
    52381 aacgcgctgg cgaaggggct gattgataaa gatgaagccg ctattctggt gaaagctgaa
    52441 gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct gg
//
LOCUS       DAZKGS010000014        51792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKGS010000014 DAZKGS010000000
VERSION     DAZKGS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(105..1469)
                     /gene="mnmE"
                     /locus_tag="ACHOQY_000866"
     CDS             complement(105..1469)
                     /gene="mnmE"
                     /locus_tag="ACHOQY_000866"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9677013.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1575..3221)
                     /gene="yidC"
                     /locus_tag="ACHOQY_000867"
     CDS             complement(1575..3221)
                     /gene="yidC"
                     /locus_tag="ACHOQY_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9677014.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3224..3481)
                     /gene="yidD"
                     /locus_tag="ACHOQY_000868"
     CDS             complement(3224..3481)
                     /gene="yidD"
                     /locus_tag="ACHOQY_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9677015.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3445..3804)
                     /gene="rnpA"
                     /locus_tag="ACHOQY_000869"
     CDS             complement(3445..3804)
                     /gene="rnpA"
                     /locus_tag="ACHOQY_000869"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9677016.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3821..3961)
                     /gene="rpmH"
                     /locus_tag="ACHOQY_000870"
     CDS             complement(3821..3961)
                     /gene="rpmH"
                     /locus_tag="ACHOQY_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9677017.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4191..4271)
                     /locus_tag="ACHOQY_000871"
     CDS             complement(4191..4271)
                     /locus_tag="ACHOQY_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677018.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4568..5971
                     /gene="dnaA"
                     /locus_tag="ACHOQY_000872"
     CDS             4568..5971
                     /gene="dnaA"
                     /locus_tag="ACHOQY_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9677019.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5976..7076
                     /gene="dnaN"
                     /locus_tag="ACHOQY_000873"
     CDS             5976..7076
                     /gene="dnaN"
                     /locus_tag="ACHOQY_000873"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9677020.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7076..8149
                     /gene="recF"
                     /locus_tag="ACHOQY_000874"
     CDS             7076..8149
                     /gene="recF"
                     /locus_tag="ACHOQY_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9677021.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8178..10592
                     /gene="gyrB"
                     /locus_tag="ACHOQY_000875"
     CDS             8178..10592
                     /gene="gyrB"
                     /locus_tag="ACHOQY_000875"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9677022.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10832..11230
                     /gene="yidB"
                     /locus_tag="ACHOQY_000876"
     CDS             10832..11230
                     /gene="yidB"
                     /locus_tag="ACHOQY_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9677023.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11345..12157
                     /gene="yidA"
                     /locus_tag="ACHOQY_000877"
     CDS             11345..12157
                     /gene="yidA"
                     /locus_tag="ACHOQY_000877"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9677024.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12203..12859)
                     /gene="yidX"
                     /locus_tag="ACHOQY_000878"
     CDS             complement(12203..12859)
                     /gene="yidX"
                     /locus_tag="ACHOQY_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP9677025.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13137..13826
                     /gene="dgoR"
                     /locus_tag="ACHOQY_000879"
     CDS             13137..13826
                     /gene="dgoR"
                     /locus_tag="ACHOQY_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP9677026.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13823..14701
                     /gene="dgoK"
                     /locus_tag="ACHOQY_000880"
     CDS             13823..14701
                     /gene="dgoK"
                     /locus_tag="ACHOQY_000880"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP9677027.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14685..15302
                     /gene="dgoA"
                     /locus_tag="ACHOQY_000881"
     CDS             14685..15302
                     /gene="dgoA"
                     /locus_tag="ACHOQY_000881"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP9677028.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15299..16447
                     /gene="dgoD"
                     /locus_tag="ACHOQY_000882"
     CDS             15299..16447
                     /gene="dgoD"
                     /locus_tag="ACHOQY_000882"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP9677029.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16522..17859
                     /gene="dgoT"
                     /locus_tag="ACHOQY_000883"
     CDS             16522..17859
                     /gene="dgoT"
                     /locus_tag="ACHOQY_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677030.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17856..18920)
                     /gene="cbrA"
                     /locus_tag="ACHOQY_000884"
     CDS             complement(17856..18920)
                     /gene="cbrA"
                     /locus_tag="ACHOQY_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9677031.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            19021..20235
                     /gene="yidR"
                     /locus_tag="ACHOQY_000885"
     CDS             19021..20235
                     /gene="yidR"
                     /locus_tag="ACHOQY_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9677032.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20237..20569)
                     /gene="yidQ"
                     /locus_tag="ACHOQY_000886"
     CDS             complement(20237..20569)
                     /gene="yidQ"
                     /locus_tag="ACHOQY_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9677033.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20809..20881
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20875..21288
                     /gene="ibpA"
                     /locus_tag="ACHOQY_000887"
     CDS             20875..21288
                     /gene="ibpA"
                     /locus_tag="ACHOQY_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9677034.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21400..21828
                     /gene="ibpB"
                     /locus_tag="ACHOQY_000888"
     CDS             21400..21828
                     /gene="ibpB"
                     /locus_tag="ACHOQY_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9677035.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22025..23686
                     /gene="yidE"
                     /locus_tag="ACHOQY_000889"
     CDS             22025..23686
                     /gene="yidE"
                     /locus_tag="ACHOQY_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9677036.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23683..24399)
                     /gene="yidP"
                     /locus_tag="ACHOQY_000890"
     CDS             complement(23683..24399)
                     /gene="yidP"
                     /locus_tag="ACHOQY_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9677037.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24695..26311
                     /locus_tag="ACHOQY_000891"
     CDS             24695..26311
                     /locus_tag="ACHOQY_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9677038.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26311..27125
                     /locus_tag="ACHOQY_000892"
                     /pseudo
     CDS             26311..27125
                     /locus_tag="ACHOQY_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27122..28015)
                     /gene="yidL"
                     /locus_tag="ACHOQY_000893"
     CDS             complement(27122..28015)
                     /gene="yidL"
                     /locus_tag="ACHOQY_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9677039.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28182..29897
                     /gene="yidK"
                     /locus_tag="ACHOQY_000894"
     CDS             28182..29897
                     /gene="yidK"
                     /locus_tag="ACHOQY_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9677040.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29894..31387
                     /gene="yidJ"
                     /locus_tag="ACHOQY_000895"
     CDS             29894..31387
                     /gene="yidJ"
                     /locus_tag="ACHOQY_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9677041.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31434..31883)
                     /gene="yidI"
                     /locus_tag="ACHOQY_000896"
     CDS             complement(31434..31883)
                     /gene="yidI"
                     /locus_tag="ACHOQY_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP9677042.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31993..32340
                     /gene="yidH"
                     /locus_tag="ACHOQY_000897"
     CDS             31993..32340
                     /gene="yidH"
                     /locus_tag="ACHOQY_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9677043.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32330..32692
                     /gene="yidG"
                     /locus_tag="ACHOQY_000898"
     CDS             32330..32692
                     /gene="yidG"
                     /locus_tag="ACHOQY_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9677044.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32689..33186
                     /gene="yidF"
                     /locus_tag="ACHOQY_000899"
     CDS             32689..33186
                     /gene="yidF"
                     /locus_tag="ACHOQY_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9677045.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33194..34378)
                     /gene="emrD"
                     /locus_tag="ACHOQY_000900"
     CDS             complement(33194..34378)
                     /gene="emrD"
                     /locus_tag="ACHOQY_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9677046.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34460..34534
                     /gene="ysdE"
                     /locus_tag="ACHOQY_000901"
     CDS             34460..34534
                     /gene="ysdE"
                     /locus_tag="ACHOQY_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9677047.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34797..34886)
                     /gene="tisB"
                     /locus_tag="ACHOQY_000902"
     CDS             complement(34797..34886)
                     /gene="tisB"
                     /locus_tag="ACHOQY_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP9677048.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35451..35549
                     /gene="ivbL"
                     /locus_tag="ACHOQY_000903"
     CDS             35451..35549
                     /gene="ivbL"
                     /locus_tag="ACHOQY_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9677049.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35655..37343
                     /gene="ilvB"
                     /locus_tag="ACHOQY_000904"
     CDS             35655..37343
                     /gene="ilvB"
                     /locus_tag="ACHOQY_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9677050.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37347..37637
                     /gene="ilvN"
                     /locus_tag="ACHOQY_000905"
     CDS             37347..37637
                     /gene="ilvN"
                     /locus_tag="ACHOQY_000905"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9677051.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37712..38302
                     /gene="uhpA"
                     /locus_tag="ACHOQY_000906"
     CDS             37712..38302
                     /gene="uhpA"
                     /locus_tag="ACHOQY_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9677052.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38302..39804
                     /gene="uhpB"
                     /locus_tag="ACHOQY_000907"
     CDS             38302..39804
                     /gene="uhpB"
                     /locus_tag="ACHOQY_000907"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9677053.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39814..41133
                     /gene="uhpC"
                     /locus_tag="ACHOQY_000908"
     CDS             39814..41133
                     /gene="uhpC"
                     /locus_tag="ACHOQY_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9677054.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41389..42780
                     /gene="uhpT"
                     /locus_tag="ACHOQY_000909"
     CDS             41389..42780
                     /gene="uhpT"
                     /locus_tag="ACHOQY_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9677055.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42825..44591)
                     /gene="adeD"
                     /locus_tag="ACHOQY_000910"
     CDS             complement(42825..44591)
                     /gene="adeD"
                     /locus_tag="ACHOQY_000910"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9677056.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            44766..46100
                     /gene="adeQ"
                     /locus_tag="ACHOQY_000911"
                     /gene_synonym="yicO"
     CDS             44766..46100
                     /gene="adeQ"
                     /locus_tag="ACHOQY_000911"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP9677057.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            46153..46605
                     /gene="yicN"
                     /locus_tag="ACHOQY_000912"
     CDS             46153..46605
                     /gene="yicN"
                     /locus_tag="ACHOQY_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9677058.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            46816..48006
                     /gene="nepI"
                     /locus_tag="ACHOQY_000913"
     CDS             46816..48006
                     /gene="nepI"
                     /locus_tag="ACHOQY_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9677059.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(48047..48340)
                     /gene="yicS"
                     /locus_tag="ACHOQY_000914"
     CDS             complement(48047..48340)
                     /gene="yicS"
                     /locus_tag="ACHOQY_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9677060.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            48562..49380
                     /gene="nlpA"
                     /locus_tag="ACHOQY_000915"
     CDS             48562..49380
                     /gene="nlpA"
                     /locus_tag="ACHOQY_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9677061.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(49384..50307)
                     /gene="yicL"
                     /locus_tag="ACHOQY_000916"
     CDS             complement(49384..50307)
                     /gene="yicL"
                     /locus_tag="ACHOQY_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9677062.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(50418..51602)
                     /gene="setC"
                     /locus_tag="ACHOQY_000917"
     CDS             complement(50418..51602)
                     /gene="setC"
                     /locus_tag="ACHOQY_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFP9677063.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT        12097 a        13059 c        13918 g        12718 t
ORIGIN      
        1 acgtaagaat tttaatgttt aaaaaaaggg ttgaggaata acaggagtgc taacaatgaa
       61 gcgaagccgc atctgacagt cagaatacgg cttcgtaagc gcggttactt accaatacag
      121 aagctggaga aaatccgccc cagcaggtcg tctgaagtaa attccccggt gatttcgctt
      181 aagttctgct gtgccaggcg caactcttcc gccagcagtt cacccgccca ggcacctaac
      241 agttgcgctt taccttgctg caaatgctcc gctgcctgtt ccagcgcctg taagtggcga
      301 cggcgcgcca ggaaaccgcc ttccatgttg gtgtcaaagc ccatgctctg tttgagatgg
      361 ttacgcagca cgtccacgcc ttcaccagtc cttgccgaga gacgaattaa cgcgtgaccg
      421 ttcacttcac tcattcccag cgtttcgccg gtgatatcgg ctttattgcg caccacggtg
      481 atcggcagtt tcgctggcag acgggcgata aattccggcc agatctctgc cggatccacg
      541 gcgtctgttg tggtgccatc gaccataaac agcacccggt cggcctgttc aatttcctgc
      601 cacgcgcgct cgatcccaat acgttccact tcgtcactgg cttcacgtag cccggcggtg
      661 tcgatgatat gcagcggcat tccgtcaatg tggatatgct cacgcaacac gtcacgcgta
      721 gttccggcga tatcggttac gattgctgct tcacggccag ccagcgcgtt taacaggctc
      781 gatttaccgg cgttaggacg tccggcaatc accactttca tcccttcgcg caacaaacta
      841 ccctgacgtg cttcagcacg cactgcatca agatcggcaa taacgtcatt gagctgggct
      901 tcaattttgc cgtcggagag gaaatcgatc tcttcatcag ggaaatcaat tgccgcttcg
      961 acgtaaatgc gcaagtgggt gagggcttct accagatgat taacccgtgc ggagaatgcg
     1021 ccttgcagcg agttaagtgc cgaacgggcc gcctgttccg agctggcgtc gataagatcg
     1081 gcaatcgcct cagcctgggc taagtcgagt ttatcgtaaa gaaacgcgcg ttcggaaaac
     1141 tcaccagggc gagcaatccg caggccggga atggtcagaa tgcgttttaa cagcaggtcg
     1201 aggatcaccg gaccgccatg accctgcagt tcaagcacat cttcgccggt gaacgagttc
     1261 gggccaggga accatagcgc aatcccctga tcgagcacgc tgccgtcggc gtctttaaat
     1321 ggcaaataat cggcgtagcg cggcttaggt agtttaccca gcacggtttc ggcaacttca
     1381 cgggctttga ggccggagat gcgcaggatg ccaacaccgc cacgtcccgg aggcgtggcc
     1441 tgggctacga tagtgtcatt atcgctcatg atgttcctgt tgctttgtgt ggcggatgcg
     1501 cggtgcttat ccgccctacg aaaagaaaaa aggcggtcaa ctgaccgccc ttattttagc
     1561 gaaaactcac cgaatcagga ttttttcttc tcgcggctat gcaggccacg tttttccaga
     1621 ccacggtaaa tcagctgctg ctgaataatg gttaccaggt tgctgacgat atagtacagc
     1681 accagacctg acgggaacca caggaagaac acggtgaaga tgaccggcat aaaggtcatg
     1741 atcttctgct gcatcgggtc ggtcactgtg gtcggcgaca tcttctgaat gaagaacatc
     1801 gttacgccca tcaggatcgg caggatgtag tacgggtcct gtgccgacag gtcgtggatc
     1861 cacagtgcaa acggtgcctg acgcagttca acggaaccca tcagcatgta gtacaacgcc
     1921 aggaagattg gcatctggat cagcagcggg aagcagccgc ccagcgggtt aaccttctca
     1981 gctttgtaca gcgccatcat ttcctggctg atacgctgtt tgtcatcgcc cagacgctca
     2041 cgcattgcct gaatcttcgg ctgcagcata cgcatcttcg ccatggaggt gtactgcgct
     2101 ttggtcagcg ggtacatgat gccacgaacg ataaaggtga tgatgataat ggagaagccc
     2161 cagttaccca caaagctatg gatccatttc agcagtttga acagcggctg agagatgaac
     2221 cacaaccaac cgtaatcaac ggtcagatcc aggtgcggag caacagctgc cattttgtcc
     2281 tggatttccg ggccaaccca cagggtgctg ttcatcgcgc cagtctgacc aggctgaacc
     2341 agtaccggct gagatttata gccgatagcg gcgatgccgt tacccagatt agcggtatag
     2401 aagttgttgg taccgtcgtt atgcgggatc cacgccgtcg cgaaatactg ttgcagcatc
     2461 gccacccaac cacctttcga agagatgttc aggttttcgt tatcggcaat ggtatcgaac
     2521 ttgtatttct catacttctc gtcaggcgtg gagtacgccg cgccacggaa ggtgtgcagt
     2581 gcgaagttgc tgcttccggt atcgagatgc ggtggcagag tgatggattg cttcaactga
     2641 ccaaaggtgg agatttccag cggtttctcg ccagcgttct gcacgttgta gttgacgttg
     2701 acagcgtaat caccacgttt caggacaaac gttttggtaa acgtgttgcc tgccgcgtcg
     2761 gtatacgtca tcggcacctg cagttcgttt tgaccttcag ccagcacata agcgtctttt
     2821 tcaacgttat acagcggacg cgggccgtta gccgggttat ccgggccatc acgaccggtc
     2881 agaccgctct gtgcctgata aataaactgc ggtgaagttt ccagcagctg gaacggctgg
     2941 gtagagttca gctctttcgg gtaagcaggc agcagagctt gctcaacatc accaccacgg
     3001 gtgttgatgg tcagatcaag cacgtcggtc ttaaccgaga tcagtttccc ctggccactg
     3061 gccggtacgc cctggtcggc ggcgctaccc gctgcggtgg tcgttgtctg cgtggtctgt
     3121 tgggcctgag gttgcgggtt tttatcctgc tcccaggctt gccagatcat gaaagacacg
     3181 aacagcaaag cgatgactaa aagattgcgt tgcgaatcca tcgttagtgt tctctggtat
     3241 caaatggtcc gggcgggacg ggatcgtcac caccagggtg taaagggtgg cattttaata
     3301 cgcgtttcac cgtcaaccaa ctgcctttta tcactccaaa cctgcgcaat gcctcaattc
     3361 cgtagcttga acaggttgga gtgaaacgac aatgcggccc gagtagcgga ctaatcaggc
     3421 gttgatagac ccgaatgagg gctatcagga cccgcgagcc aggcgacagt ggcggcgcca
     3481 taatttttcc aacgcttccg agagagcacg gttatcgagg tcggcaaccc cttttttcgc
     3541 caccaccacg aaatccatag ccgggagttc atgttggcgc agacggaagc tttcacgcgt
     3601 cagacgttta atccgattgc gttcatgggc gcgtcgaacg tttttcttgg cgactgtaag
     3661 accgatacgg ggatgcccca gcgaattcag gcggccgaga atggtaattt gcggcgtgcc
     3721 agcccgttgt ggctgctgga agacgaatgt gaattgactg ggagttaaca agcgtaactc
     3781 cctgggaaat gcgagcttaa ccactcaggg gttagcttta ttacttagaa acggtcagac
     3841 gagcgcggcc tttagcacga cgacgtgcca gaacctgacg accattttta gtagccatac
     3901 gagcacggaa gccgtgagaa cggttgcgct tcagtacaga cggttgaaaa gtgcgtttca
     3961 tggcgatttc tacctaaact tgaataaatt caatggcttt attggatatc cgccgaaaaa
     4021 tgaaacgatg gacaccgaag ccatgggtga ttaaagaggc cggattgtaa taattgtaca
     4081 ctccggagtc aattctcttt ccttatttac cgcgcttttc cgcacctttt cgcagggaaa
     4141 atgtacgacc tcacaccagt gaaaaccagc atggcgcgcc gggtggagga ttatacgggc
     4201 tgatgggtaa agcgcaagga tcgtcctgga tctttattag atcgattaag ccaatttttg
     4261 tctatggtca ttaaattttc caatatgcgg cgtaaatcgt gcccgcctcg cggcaggatc
     4321 gtttacactt agcgaggtct ggaaagtcct gtggataaat cgggaaaatc tgtgagaaac
     4381 agaagatctc ttgcgcagtt taggctatga tccgcggtcc cgatcgtttt gcaggatctt
     4441 gatcgggcat ataaccgcag acagcggttc gtgcgtcacc ctcaagcagg gtcttttcga
     4501 cgtacgtcaa caatcatgaa tgtttcagcc ttagtcatta tcgacttttg ttcgagtgga
     4561 gtccgccgtg tcactttcgc tttggcagca gtgtcttgcc cgattgcagg atgagttacc
     4621 agccacagaa ttcagtatgt ggatacgccc attgcaggcg gaactgagcg ataacacgct
     4681 ggccctgtac gcgccaaacc gttttgtcct cgattgggta cgggacaagt accttaataa
     4741 tatcaatgga ctgctaacca gtttctgcgg agcggatgcc ccacagctgc gttttgaagt
     4801 cggcaccaaa ccggtgacgc aaacgccaca agcggcagtg acgagcaacg tcgcggcccc
     4861 tgcacaggtg gcgcaaacgc agccgcaacg tgctgcgcct tctacgcgct caggttggga
     4921 taacatcccg gccccggcag aaccgaccta tcgttctaac gtaaacgtca aacacacgtt
     4981 tgataacttc gttgaaggta aatctaacca actggcgcgc gcggcggctc gccaggtggc
     5041 ggataaccct ggcggtgcct ataacccgtt gttcctttat ggcggcacgg gtctgggtaa
     5101 aactcacctg ctgcatgcgg tgggtaacgg cattatggcg cgcaagccga atgccaaagt
     5161 ggtttatatg cactccgagc gctttgttca ggacatggtt aaagccctgc aaaacaacgc
     5221 gatcgaagag tttaaacgct actaccgttc cgtagatgca ctgctgatcg acgatattca
     5281 gttttttgct aataaagaac gatctcagga agagtttttc cataccttca acgccctgct
     5341 ggaaggtaat caacagatca ttctcacctc ggatcgctat ccgaaagaga tcaacggcgt
     5401 tgaggatcgt ttgaaatccc gcttcggttg gggactgact gtggcaatcg aaccgccaga
     5461 gctggaaacc cgtgtggcga tcctgatgaa aaaggccgac gaaaacgaca ttcgtttgcc
     5521 gggcgaagtg gcgttcttta tcgccaagcg tctacgatct aacgtacgtg agctggaagg
     5581 ggcgctgaac cgcgtcattg ccaatgccaa ctttaccgga cgggcgatca ccatcgactt
     5641 cgtgcgtgag gcgctgcgcg acttgctggc attgcaggaa aaactggtca ccatcgacaa
     5701 tattcagaag acggtggcgg agtactacaa gatcaaagtc gcggatctcc tttccaagcg
     5761 tcgatcccgc tcggtggcgc gtccgcgcca gatggcgatg gcgctggcga aagagctgac
     5821 taaccacagt ctgccggaga ttggcgatgc gtttggtggt cgtgaccaca cgacggtgct
     5881 tcatgcctgc cgtaagatcg agcagttgcg tgaagagagc cacgatatca aagaagattt
     5941 ttcaaattta atcagaacat tgtcatcgta aacctatgaa atttaccgta gaacgtgagc
     6001 atttattaaa accgctacaa caggtgagcg gtccgttagg tggtcgtcct acgctaccga
     6061 ttctcggtaa tctgctgtta caggttgctg acggtacgtt gtcgctgacc ggtactgatc
     6121 tcgagatgga aatggtggca cgtgttgcgc tggttcagcc acacgagcca ggagcgacga
     6181 ccgttccggc gcgcaaattc tttgatatct gccgtggtct gcctgaaggc gcggaaattg
     6241 ccgtgcagct ggaaggtgaa cggatgctgg tacgctccgg gcgtagccgt ttttcgctgt
     6301 ctaccctgcc agcggcggat ttcccgaacc tcgatgactg gcagagtgaa gtcgaattta
     6361 ccctgccgca ggcaacgatg aagcgtctga ttgaagcgac ccagttttcg atggcgcatc
     6421 aggacgttcg ctattactta aatggtatgc tgtttgaaac cgaaggtgaa gaactgcgca
     6481 ccgtggcaac cgacggccac cgtctggcgg tctgttcaat gccaattggt caatctttgc
     6541 caagccattc ggtgatcgta ccgcgtaaag gcgtgattga actgatgcgt atgctcgacg
     6601 gcggcgacaa tccgctgcgc gtgcagattg gcagcaacaa tattcgcgcc cacgttggcg
     6661 actttatctt cacctccaaa ctggtggatg gtcgcttccc ggattaccgc cgcgttctgc
     6721 cgaagaatcc ggacaaacat ctggaagctg gctgcgatct gctcaagcag gcgtttgccc
     6781 gtgcggcaat tctctctaac gagaaattcc gcggcgtgcg cctgtatgtc agcgaaaacc
     6841 agctgaaaat caccgccaac aacccggaac aggaagaagc ggaagagatc ctcgacgtta
     6901 cctatagcgg tgcggagatg gaaatcggct tcaacgtcag ctatgtgctg gatgttctga
     6961 acgcgctgaa atgcgaaaac gtccgcatga tgctgaccga ttcggtttcc agcgtgcaga
     7021 ttgaagatgc cgcatcacag tcggctgcct atgttgtcat gccaatgaga ctgtaatgtc
     7081 cctcacccgc ttgttgatcc gcgatttccg caacattgaa accgcggatc tcgctttatc
     7141 tcccggcttt aactttctgg taggtgccaa cggcagtggc aaaaccagcg tgctggaagc
     7201 catctatacg ctcggccatg gtcgggcgtt tcgcagtttg cagattggtc gcgtcattcg
     7261 ccatgagcag gaggcatttg ttctccatgg gcgattacag ggcgaagagc gcgagacggc
     7321 gattggctta accaaggaca aacagggcga cagcaaagtc cgcatcgacg gtactgacgg
     7381 gcataaagtc gcggaactgg cgcacctgat gccaatgcag ctgataacgc cagaagggtt
     7441 tactttactc aacggcggcc ccaaatacag aagagcattc ctcgactggg gatgctttca
     7501 caacgaaccc ggatttttca ccgcctggag caatctcaag cgattgctca agcagcgcaa
     7561 tgcggcgctg cgccaggtga cacgttacga acagctacgc ccgtgggata aagaactgat
     7621 cccgctggcg gagcaaatca gcacctggcg cgcggagtat agcgccggta tcgcggccga
     7681 tatggccgat acctgtaagc aatttctccc tgagttttct ctgactttct ctttccagcg
     7741 cggctgggag aaagagacag aatatgctga ggtgctggaa cgtaattttg aacgcgatcg
     7801 ccagctaacc tacaccgcgc atggcccgca taaagcggac ttacgcattc gcgccgacgg
     7861 tgcgccggtg gaagatacct tatcgcgtgg gcagcttaag ctgttgatgt gcgccttacg
     7921 tctggcgcaa ggagagttcc tcacccgtga aagcgggcgg cggtgtctct acctgataga
     7981 tgattttgcc tctgagcttg atgatgagcg tcgtgggttg cttgccagcc gcttaaaagc
     8041 gacgcaatca caggtctttg tcagcgcgat cagtgctgaa cacgttatag acatgtcgga
     8101 cgaaaattcg aagatgttta ccgtggaaaa gggtaaaata acggattaac ccaagtataa
     8161 atgagcgaga aacgttgatg tcgaattctt atgactcctc cagtatcaaa gtcctgaaag
     8221 ggctggatgc ggtgcgtaag cgcccgggta tgtatatcgg cgacacggat gacggcaccg
     8281 gtctgcacca catggtattc gaggtggtag ataacgctat cgacgaagcg ctcgcgggtc
     8341 actgtaaaga aattatcgtc accattcacg ccgacaactc tgtctctgta caggatgacg
     8401 ggcgcggcat tccgaccggt attcacccgg aagagggcgt atcggcggcg gaagtgatca
     8461 tgaccgttct gcacgcaggc ggtaaattcg acgataactc ctataaagtg tccggcggtc
     8521 tgcacggcgt tggtgtttcg gtagtaaacg ccctgtcgca aaaactggag ctggttatcc
     8581 agcgcgaggg taaaattcac cgtcagatct acgaacacgg tgtaccgcag gccccgctgg
     8641 cggttaccgg cgagactgaa aaaaccggca ccatggtgcg tttctggcct agcctcgaaa
     8701 ctttcaccaa tgtgaccgag ttcgaatatg aaattctggc gaaacgtctg cgtgagttgt
     8761 cgttcctcaa ctccggcgtt tccattcgtc tgcgcgacaa gcgcgacggc aaagaagacc
     8821 acttccacta tgaaggcggc atcaaggcgt tcgttgaata tctgaacaag aacaaaacgc
     8881 cgatccaccc gaatatcttc tacttctcca ctgaaaaaga cggtattggc gtcgaagtgg
     8941 cgttgcagtg gaacgatggc ttccaggaaa acatctactg ctttaccaac aacattccgc
     9001 agcgtgacgg cggtactcac ctggcaggct tccgtgcggc gatgacccgt accctgaacg
     9061 cctacatgga caaagaaggc tacagcaaaa aagccaaagt tagcgccacc ggtgacgatg
     9121 cgcgtgaagg cctgattgcg gtcgtttccg tgaaagtgcc ggacccgaaa ttctcctccc
     9181 agaccaaaga caaactggtt tcttctgagg tgaaatcagc ggttgaacag cagatgaacg
     9241 aactgctggc agaatacctg ctggaaaacc caaccgacgc gaaaatcgtg gttggcaaaa
     9301 ttatcgatgc tgcccgtgcc cgtgaagcgg cgcgtcgcgc gcgtgaaatg acccgccgta
     9361 aaggtgcgct cgacttagcg ggcctgccgg gcaaactggc agactgccag gaacgcgatc
     9421 cggcgctttc cgaactgtac ttggtggaag gggactccgc gggcggctct gcgaagcagg
     9481 ggcgtaaccg caagaaccag gcgattctgc cgctgaaggg taaaatcctc aacgtcgaga
     9541 aagcgcgctt cgataagatg ctctcttctc aggaagtggc gacgcttatc accgcgcttg
     9601 gctgtggtat cggtcgtgac gagtacaacc cggacaaact gcgttatcac agcatcatca
     9661 tcatgaccga tgcggacgtc gacggctcgc acattcgtac gctgctgttg accttcttct
     9721 atcgtcagat gccggaaatc gttgaacgcg gtcacgtcta catcgctcag ccgccgctgt
     9781 acaaagtgaa gaaaggcaag caggaacagt acattaaaga cgacgaagcg atggatcagt
     9841 accagatctc tatcgcgctg gatggcgcaa cgctgcacac caacgccagc gcaccggcat
     9901 tggctggcga agcgttagag aaattggtgt ctgagtacaa cgcgacgcag aaaatgatca
     9961 accgcatgga gcgtcgttat ccgaaagcaa tgctgaaaga gcttatctat cagccgacgc
    10021 tgacggaagc cgacctctct gatgagcaga ccgttacccg ctgggtgaac gcgctggtca
    10081 gcgaactgaa cgacaaagaa cagcacggca gccagtggaa gtttgatgtc cacaccaatg
    10141 ccgaacaaaa cctgttcgag ccgattgttc gcgtgcgtac ccacggtgtg gatactgact
    10201 atccgctgga tcacgagttt atcaccggtg gcgaatatcg tcgtatctgc acgctgggtg
    10261 agaaactgcg tggcttgctg gaagaagatg cgtttatcga acgtggcgag cgtcgtcagc
    10321 cggtagccag cttcgagcag gcgctggact ggctggtgaa agagtcccgt cgcggcctct
    10381 ccatccagcg ttataaaggt ctgggcgaga tgaacccgga acagctgtgg gaaaccacca
    10441 tggacccgga aagccgtcgt atgctgcgcg ttaccgttaa ggatgcgatt gccgctgacc
    10501 agttgttcac cacgctgatg ggcgacgccg ttgaaccgcg ccgtgcgttt attgaagaga
    10561 acgccctgaa agcggcgaat atcgatattt aatggcgcta accatgcgag cgtgcctgat
    10621 gcgctacgct tatcaggcct acgaaaatct tgcaatgtat tgaattttca tgattttgta
    10681 ggccggataa ggcgttcacg ccgcatccgg caagaagcta taagaaaagg gcggagatca
    10741 tctccgccct ttttatttct gcaatccgca attatcatcc cgcaaatcta tactttttgg
    10801 gtcaacacgt aacgctttac aggtgattat catggggctt tttgatgaag ttgtcggtgc
    10861 ctttctgaaa ggcgatgcgg ggaaatatca ggctatttta agttgggttg aggagcaggg
    10921 cggcattcag gtgctgctgg aaaaactgca aagtggcggc ttaggggcca ttctctcaac
    10981 ctggctgagt aatcaacagg gcaatcaatc ggttagtggc gagcaactgg aatcggcgct
    11041 cggcacaaat gcggtgtccg atcttggaca aaaacttggc gtggatacca gtacagcttc
    11101 cagtttactg gcagaacaat tgccgaagat tattgatgcg ctctcaccgc aaggtgaagt
    11161 gtctccacaa gccaataacg atctgctttc cgcaggcatg gaactgctga aagggaaact
    11221 cttccgctaa gcaaatgggg agcatgcaac ccgcgttgca tccccctttt ccctcgggtg
    11281 ttgtactgat ttttgagcgg aatcgcgtta gcatgggtca ggaactaatc tacctgggga
    11341 actcatggct atcaaactca ttgctatcga tatggatggc acccttctgc tacccgatca
    11401 caccatttca cccgccgtta aaaatgcgat tgccgcagct cgcgcccgtg gcgtgaatgt
    11461 cgtgctaacg acgggtcgcc cttatgcagg tgttcacaat tacctgaaag agctgcatat
    11521 ggaactgccg ggcgactact gcattactta taacggcgcg ctggtacaga aggccgctga
    11581 tggtagcacc gtggcgcaaa ccgctctcag ctatgacgac taccgtttcc tggaaaaact
    11641 ctctcgcgaa gtcggttctc atttccacgc cctggaccgc accacgctgt acaccgccaa
    11701 ccgtgatatc agctactaca cggtgcatga atccttcgtt gccacaattc cgctggtgtt
    11761 ctgcgaagcg gagaaaatgg accccaatac ccagttcctg aaagtgatga tgattgatga
    11821 acccgccatc ctcgaccagg ctatcgcgcg tattccgcag gaagtgaaag agaaatatac
    11881 cgtgctgaaa agtgcgccgt acttcctcga aatcctcgat aaacgcgtta acaaaggtac
    11941 gggggtgaaa tcactggccg acgtgttagg tattaaaccg gaagaaatca tggcgattgg
    12001 cgatcaggaa aacgacatcg caatgattga atatgcaggc gtcggtgtgg cgatggataa
    12061 cgctattcct tcggtgaaag aagtggcgaa ctttgtcacc aaatccaacc ttgaagatgg
    12121 cgtggcgttt gctattgaga agtatgtgct gaattaatca gtggacgggc aaacagcata
    12181 aacgcgtttg cccgcttact gattattggt ttactgcata tacaaccaga aattgcagcg
    12241 gtgcagtaat gatgtccagc gtaacagtca ctggcagtaa tgctgcggaa acaccatccg
    12301 gaatgcctct gggtgaatag tattcataaa atcgcacttt aaacggatga tagaacgcca
    12361 tcacctttga gtaatccatg tttttatttt tctcatggat tgtgcctttt aggttttcca
    12421 gtgacctggt acagtgttga acaccacagg caaaaccata atgcgttgcc agtgcctttt
    12481 cttcttcgtt attccagtgg tagtaaagcg atattgtgcc ggtaaatttc tttttccccg
    12541 catttaaaac aaatcttgcg tcatcggcaa cctgaatatt gtgccggtta agtgctggat
    12601 cattgagcat cttaacgatg tcatcggcac catcagtgat aaggtaatcc agcgattccc
    12661 ctacaaaaac atagcctttt gttccattac tatcttttgc ctgcgatagc ccagtgatgg
    12721 tgtctgactg cgattgatac tttcctgaac taccctttgc ggtatgggaa accagagcat
    12781 acgagataca gcctgaaagc atcagcgcca gtgggaataa accggcagcc ttaaaaaatc
    12841 ccttaaaatt caacttcatg attaatcctt taaacgagta gctaagccgt tgaaagcata
    12901 ctcttctttc cctcctgttt agcgcctgat atcccttttc agatttctgc ccgacgcatg
    12961 tcattttttt atgcattgtt ctttttgtga tctaaattgt agtacaacaa tataagtttg
    13021 tactacatta cgcacacggc aaacgcgaac gtcatcacgc tggtactaca aagttgccgc
    13081 gttatgcatc gatcggggta aagtagagaa gaacatacag agcacaagga ctctccatga
    13141 ctctcaataa aaccgatcgc attgtcatta cgctgggtaa acagattgtt cacggcaaat
    13201 acgtacctgg ctcgccactt ccggctgagg cggagctctg tgaagagttt gcaacctcgc
    13261 gcaacatcat ccgtgaggtg ttccgttcgt tgatggcgaa acggctgatt gaaatgaaac
    13321 gttatcgcgg cgcgtttgtg gcaccgcgta accagtggaa ttacctcgac actgacgtac
    13381 tgcaatgggt gctggaaaac gactacgacc cacggcttat cagtgccatg agcgaagtgc
    13441 gaaatctggt ggaaccggcg attgcccgtt gggcagcaga gcgcgcgact tccagcgatc
    13501 tggcgcagat tgaatcggcg ctgaacgaga tgattgccaa caatcaggac cgcgaagcgt
    13561 ttaacgaagc ggatattcgc taccacgagg cggtgctgca gtcggtacat aacccggtgt
    13621 tacagcaact tagcattgcg atcagttcgt tgcagcgggc ggtttttgaa cgaacctgga
    13681 tgggcgatga ggccaacatg ccgcaaacgc tccaggaaca taaggcgctg ttcgatgcaa
    13741 tacggcatca ggacggcgat gcggcagagc aggcggcgct taccatgatc gccagctcga
    13801 cacgaaggtt aaaggaaatc acatgacagc tcgctacatc gcaattgact ggggatcgac
    13861 caatcttcgc gcctggcttt atcagggcga ccactgcctg gagagcaggc aatcagaagc
    13921 aggcgtcacg cgcctgaacg gaaaatctcc ggctgcggtg ttagcagaag tcacgaccga
    13981 ctggcgtgaa gagaatacgc cagtggtaat ggcaggaatg gtcggcagca atgtcggctg
    14041 gaaagttgct ccgtatttat ctgttcctgc ccgtttttcg tctattggcg aacaattaac
    14101 gtctgttggc gacaatatct ggattattcc cggattatgc gtctctcatg acgataacca
    14161 caatgtgatg cgcggcgaag aaacacaatt gatcggcgcg cgaactctgg ctccttcctc
    14221 tctttatgtt atgcccggta cgcattgcaa atgggtgcag gccgatagcc agcaaatcaa
    14281 cgattttcgc accgtgatga ccggtgaatt acatcattta ctgttaaatc actcattgat
    14341 tggcgcaggt ttgccgccgc aggaaaactc tgccgatgcc ttcgcggctg gccttgagcg
    14401 cggccttaat gcgcccgcca tattgccgca gctttttgaa gttcgcgcct cgcatgtgct
    14461 gggaacactt ccccgcgaac aggtcagcga atttctctct ggtttgttga ttggcgcaga
    14521 ggttgccagt atgcgcgact atgtgaccca tcaacacgcc atcacccttg tcgccggaac
    14581 atcgctgacc gcgcgctacc agcaagcctt tcaggcgatg ggttgcgacg tgacggcggt
    14641 ggcgggcgac acggcatttc aggctggtat aaggagcatc gctcatgcag tggcaaacta
    14701 aacttccgct gatcgccatt ttgcgcggca ttaagcccga cgaggcgctg gcgcatgttg
    14761 gcgcggtgat tgacgccggg ttcgacgcgg ttgaaatccc gctgaattcc ccacaatggg
    14821 agcaaagcat tcccgccatc gttgatgcgt atggcgacaa ggcgttgatt ggcgcaggta
    14881 cggtactgaa acctgaacag gtcgatgcgc tcgccaggat gggttgtcag ctcatcgtta
    14941 cgcccaatat ccatagtgaa gtgatccgcc gtgcggtggg ctacggcatg accgtctgcc
    15001 ccggctgcgc gacggcgacc gaagccttta ccgcgctcga agcgggcgcg caggcgctga
    15061 aaatatttcc gtcatcggct tttggtccgc aatacatcaa agcgttaaaa gcggtattgc
    15121 catcggacat cgcagtcttt gccgttggcg gcgtgacgcc agaaaacctg gcgcagtgga
    15181 tagacgcagg ttgtgctggg gcgggcttag gcagcgatct ctatcgcgcc gggcagtccg
    15241 tagaacgcac cgcgcagcag gcagcagcat ttgttaaggc gtatcgagag gcagtgcaat
    15301 gaaaatcacc aaaattacca cgtatcgttt acctccccgc tggatgttcc tgaaaattga
    15361 aaccgatgaa ggcgtggtcg gttggggcga gcccgtgatt gaaggccgcg cccgtacggt
    15421 ggaagcggca gttcacgagc tgggtgacta tttgattggt caggatcctt cgcgcatcaa
    15481 tgacttatgg caagtgatgt atcgcgccgg attttatcgt ggcggtccaa tcctgatgag
    15541 cgccattgcc gggatcgacc aggcgttatg ggatatcaaa ggcaaagtgc tgaatgcgcc
    15601 ggtctggcaa ctgatgggcg gcctggttcg cgacaaaatt aaagcctaca gttgggtcgg
    15661 cggcgatcgt ccggcggatg ttatcgacgg cattaaaacc ctgcgcgaaa tcggcttcga
    15721 taccttcaaa ctgaacggtt gtgaagaact ggggctaatt gataactccc gcgcggtaga
    15781 tgcggcagtc aacaccgtgg cacaaattcg tgaagctttt ggcaatcaga ttgagtttgg
    15841 tcttgatttc cacggtcgcg tcagcgcgcc aatggcgaaa gtgctgatta aagaactgga
    15901 gccgtatcgc ccgctgttta ttgaagagcc ggtgctggcg gaacaagccg aatactaccc
    15961 gaaattggcg gcacaaacgc atattccact ggcggcaggt gagcgcatgt tctcacgttt
    16021 cgattttaaa cgcgtgctgg aggcaggcgg tatttcgatt ctgcaaccgg atctctccca
    16081 tgcaggcggt attaccgaat gctacaaaat tgctggaatg gcagaagcct atgatgtgac
    16141 ccttgcgccg cactgtccgc tcggaccgat tgcactggca gcttgcctgc atatcgactt
    16201 tgtttcctat aacgcggtac ttcaggaaca aagtatgggc attcattaca acaaaggcgc
    16261 ggagttactc gactttgtga aaaacaaaga agacttcagt atggttggcg gcttctttaa
    16321 accgttaacg aaaccgggct taggtgtgga aatcgacgaa gctaaagtta ttgagttcag
    16381 taaaaatgcc ccggactggc gtaatccgct ctggcgtcat gaagataaca gcgtagcaga
    16441 gtggtaattc ctgccacgta agcccctcat cgggcactaa aacagcaata caaaaatata
    16501 accctctgta aattacaggg catggtgagc ggcttcgcta tgcccaaaat ctggagaaag
    16561 ctcgctatgg atattcccgt taatgcagca aagccggggc gtcggcgtta tctgacgctg
    16621 gtgatgatct ttattacggt ggtcatttgt tatgttgacc gcgctaacct ggccgtggct
    16681 tccgcccata ttcaggaaga gttcggcatt acgaaagcgg aaatgggcta tgtattttcg
    16741 gccttcgcct ggctttatac gctatgccag atccccggcg gttggttttt agatcgcgta
    16801 ggttctcgcg tgacttattt tattgcgata tttggttggt cagtggcgac tttattccag
    16861 ggctttgcca cgggattaat gtcattaatt ggtctgcgtg cgataaccgg tattttcgaa
    16921 gcgcccgctt tcccgaccaa taaccggatg gtgaccagct ggttcccgga acatgaacgc
    16981 gcttccgccg ttggttttta tacgtctggt cagtttgtcg gtctggcgtt tttgactccg
    17041 ctgctgatct ggattcagga gatgttgagc tggcactggg tgttcattgt cactggtggt
    17101 atcggcatta tctggtcgct gatttggttt aaggtttatc agccgccgcg cctgaccaaa
    17161 ggtatcagca aagctgaact ggattacatt cgtgatggcg gcggtctggt ggatggtgat
    17221 gcgccggtga agaaagaggc gcgtcagccg ttaacagcca aagactggaa actggtgttc
    17281 catcgtaaac tgatcggcgt ttatcttggg caatttgcgg tggcttctac actgtggttt
    17341 ttcttaacct ggttcccgaa ctatttaacc caggaaaaag gaatcacggc gctgaaagca
    17401 ggctttatga ccacggtgcc attcctcgcg gcgtttgtcg gcgtcctgct ctctggctgg
    17461 gtcgcggatc tgctggtacg taagggcttt tcactgggct ttgcgcgtaa aacgccgatt
    17521 atctgcggct tgctgatctc cacctgcatt atgggcgcta actacaccaa cgatccgatg
    17581 atgattatgt gcctgatggc gctggcattc ttcggcaacg gttttgcttc gattacctgg
    17641 tcgctggtct cttctctggc accgatgcgc ctgattggtt taaccggcgg cgtgtttaac
    17701 ttcgccggtg gtctgggcgg catcaccgtt ccgctggtgg tggggtacct ggcgcagggt
    17761 tacggtttcg cacctgcact ggtttatatc tccgccgtcg cgttgattgg cgcgctctct
    17821 tacatcctgc tggtgggcga tgtgaagcgc gttggctaat ctttcaactg tggaatatgc
    17881 gccataccac tgcgcataat ccgctttctt aaagccggtg cggtcaggca tcggcttttt
    17941 actatcttgc cgaagagttt taaacgcagt ttgcgggtgg cgcgccagta agcggtattg
    18001 agcttctctg gctgcttcag taacaccgaa cgcagaatgt ctgcgctatc cagcgcatag
    18061 ctaatcccct ccagcgaact ggcgctgata aatcctgccg cttcgccaat cagaaaggcg
    18121 ttgtccttac cgcagacaaa atcctgccag cgtgagggaa acagcaccgt gcatttttcg
    18181 cttttcaccg ccttaccaaa ctggaactga aaggcgctca ttttctcttt cagcgtcgtg
    18241 aaacgcgtct gaccgtcttt cattggataa gcaccgccaa agataaaata accgtctttg
    18301 ctgatactcc atgaataaca gttagttatc gaattatcaa agatgcagga gtagaacggc
    18361 accggatgtt tctctgcgaa ccactgctgg atagcgacat atttacggat ttgatgatcc
    18421 gggtagagat gtcgccgcac catcgagttt gcgccatcgg caccgaccag atagcgggca
    18481 gtaatatgtt gctcccagcc gtctgcacga aaaatgacat gccatttatc atcctcacgc
    18541 cagatttttc gacacaggct gtcgtgataa atctcaacgc tggcggggat cagcgatttc
    18601 atccacaagt cgaaagcatg gcgattaata ttgatatagc ttcgctggta gttacgcgtg
    18661 agcgatgcgg cgacgtcgac agttttgacg ctgaaaatct gcgggttggc gatcacatcg
    18721 acaggaagcg tcagtccatc gcgaataaaa gatcgctgcg cgtccggtgc cagcagaccg
    18781 ccgcaaggtt tgctgaaacc ttcagtacca cactggtgct ttttatccag cgcgatcacc
    18841 tgcattttgc ctgctaattt tcgcgccaac gccgatcctg ccgggccgag gccaataatc
    18901 gccacgtcga aatgttccat cttttcactc acattcatca cgatggcaaa agactattgt
    18961 ctgaaagtga agtccttgtg aagtatgatg gcgggacccg ttttgtatca ggaccgcgcc
    19021 atgaaacaga taacctttgc tccccgtaat cacctgctca ccaataccaa tacctggacg
    19081 cccgacagcc agtggctggt atttgacgtg cgtccttctg gggcgtcgtt taccggcgag
    19141 accattgagc gtgtgaatat ccataccggc gaggtcgagg ttatctatcg cgcgtcacag
    19201 ggcgcacacg tcggcgtggt gaccgttcat ccgaagtcag agaaatatgt tttcatccat
    19261 ggcccggaaa atcctgatga aacatggcat tacgatttcc atcatcgacg cggagtcatt
    19321 gctgaaggcg gcaaggtgag caatctcgat gcaatggata ttaccgcgcc gtatatgcca
    19381 ggagcgctgc gcggcggcag ccatgtgcat gtctttagcc cgaacggtga aagggtgagc
    19441 tttacctata acgaccatgt aatgcatgaa ctcgatccgg cgctggattt gcgaaacgtc
    19501 ggtgttgctg cgccgtttgg cccggtcaac gtacaaaagc agcatccgcg tgaatacagc
    19561 ggtagccact ggtgcgtgct ggtgagtaaa accacgccca cgccgcagcc tggcagcgat
    19621 gaaatcaatc gtgcttatga agaaggatgg gtaggaaatc acgcgctggc gtttattggc
    19681 gacacacttt cgccaaaggg cgagaaagtg ccggagctgt ttatcgttga gttaccgcaa
    19741 gatgaagctg gctggaaagc ggcaggtgat gcgccgttaa gtggaacgga aaccaccctg
    19801 cccgcgccac cgcgcggcgt cgtgcagcga cgtttaacct ttacccacca tcgggcttat
    19861 ccggggttag tcaacgtccc gcgccactgg gtgcgctgta atccgcaggg tacgcaaatc
    19921 gcgtttttaa tgcgtgatga taacggcatt gtgcaactgt ggcttatctc gccacagggc
    19981 ggcgagccgc gccagttaac ccataacaaa acggatattc agtctgcatt taactggcat
    20041 ccgtcaggag aatggttggg ctttgtgctg gataatcgaa ttgcttgtgc ccatgcgcaa
    20101 agcggcgagg ttgagtattt aaccgaaaac cacgccaatc caccttctgc ggacgccgag
    20161 gtcttctcgc cggatggtca atggctggcg tggatggaag gtggccagct gtggatcacc
    20221 gaaactgatc gctaaatcag ttatcaggca ttctggccgg tggaataacc gcattggtgg
    20281 cttgcgcgtt ggcttcgctt ttttcaacgc gtgagcgcac cgagctgtct ttgcggaaca
    20341 catcccacgg cagtaaaagc gtgtccatca cagcggtaaa cggcatatcg agaatcgcca
    20401 gcgacttagt gccccagttg gtttcatcgt cgccaatcat ggttgcgctg gcgcgggtgc
    20461 cgggatacgt tccttctttc cctccggtat gggacatcac gctggagcat cctccgagta
    20521 atgtcattcc gctgcatatc atgaacgcta acaacacatt tcttatcata ataaattcat
    20581 ctgttgatcg tgggtgttgg cctgatgagt tatagcgatc ccttgctgaa aataacatca
    20641 tcattacgtc gcactgtggc ggctatcgca ctttaacgtt tcgtgctgcc ccctcagtct
    20701 atgcaataga ccataaactg caaaaaaaag tccgctgata aggcttgaaa agttcatttc
    20761 cagacccatt tttacatcgt agccgatgag gacgcgcctg atgggtgttc tggctacctg
    20821 acctgtccat tgtggaaggt cttacattct cgctgatttc aggagctatt gattatgcgt
    20881 aactttgatt tatccccgct ttaccgttct gctattggat ttgaccgttt gtttaaccac
    20941 ttagaaaaca accagagcca gagtaatggc ggctaccctc cgtataacgt tgaactggta
    21001 gacgaaaacc attaccgcat tgctatcgct gtggctggtt ttgctgagag cgaactggaa
    21061 attaccgccc aggataatct gctagtggtg aaaggtgctc acgccgacga acaaaaagag
    21121 cgcacctatc tgtaccaggg catcgctgaa cgcaactttg aacgcaaatt ccagttagct
    21181 gagaacattc atgttcgtgg cgctaacctg gtaaatggtt tgctgtatat cgatctcgaa
    21241 cgcgtgattc cggaagcgaa aaaaccgcgc cgtatcgaaa tcaactaatt ccctaaggcc
    21301 gcctggcgcg gcctgacatc tccatgctcg ccgttaggga gcatatgcga atcttcggat
    21361 ttgcaggtac ttactcgctt cttagaagga gaaatgacta tgcgtaactt cgatttatcc
    21421 ccactgatgc gtcaatggat cggttttgac aaactggcca acgcactgca aaacgccggt
    21481 gaaagccaga gcttcccgcc gtacaacatt gagaaaagcg acgataacca ctaccgcatt
    21541 acccttgcgc tggcaggttt ccgtcaggaa gatttagaga ttcaactgga aggtacgcgc
    21601 ctgagcgtaa aaggcacgcc ggagcagcca aaagaagaga aaaaatggct gcatcaaggg
    21661 cttatgaatc agccatttag cctgagcttt acgctggctg aaaatatgga agtctctggc
    21721 gcaaccttcg taaacggttt actgcatatt gatttaattc gtaatgagcc tgaacccatc
    21781 gcagcgcagc gtatcgctat cagcgaacgt cccgcgttaa atagctaact agctattctc
    21841 tttgccccgc cattctggtg gggctttttt tgtcttactt actcaacaat gcgttaaatt
    21901 tcgactgttt aagatatttc ggcacgttct ttcccgtgat aatgtgcgct ataaggcaaa
    21961 tctgcttcac cgattcagcc ataatccgta gcaattaaaa atgttatctc aagagaaatg
    22021 gacgatgagt gatatagcat taacggtcag tattctggct ttggtggcag tcgtcggttt
    22081 gtttatcggc aatgtcaaat ttcgcggcat aggattaggt attggcggcg tgctgtttgg
    22141 tgggatcatc gtcggccatt ttgtttctca ggcggggatg acattaagta gcgatatgct
    22201 gcatgttatt caggaatttg gcctgatcct gttcgtttat actatcggga ttcaggtagg
    22261 gccgggcttc tttgcctcat tgcgcgtctc cggattacgc ctcaacctgt ttgctgttct
    22321 gatcgtcatc atcggtggtc tggttaccgc catcctgcat aaactgtttg atattccact
    22381 gccggtagtg ctggggattt tctccggtgc ggttaccaat acgccagcgc tgggggcagg
    22441 gcagcagatt ttgcgcgact tgggtacacc aatggaaatg gtcgatcaga tggggatgag
    22501 ttacgcgatg gcgtatccat tcggcatttg cgggattttg ttcaccatgt ggatgttgcg
    22561 ggttattttc cgcgtcaatg tcgagacaga agctcagcag cacgagtctt cacgcaccaa
    22621 tggcggcgcg ctgatcaaga ctatcaatat tcgcgttgag aaccctaacc tgcatgattt
    22681 agccattaaa gatgtaccga ttctcaacgg cgacaaaatt atctgctcgc gtctgaaacg
    22741 cgaagaaacc ctaaaagttc cttcgccaga taccattatc caactgggcg atttgctgca
    22801 tctggtgggg cagccagcgg atttacataa tgcgcaactg gtgattggtc aggaggtcga
    22861 tacttcgctg tccacgaaag gcactgattt gcgcgtcgag cgtgtggtgg tcaccaatga
    22921 aaacgtgctc ggaaaacgta ttcgcgacct gcactttaaa gaacgctatg acgttgttat
    22981 ctcgcgcctg aaccgtgccg gggtcgaact ggtcgccagt ggcgatatca gcctgcagtt
    23041 cggcgatatc ctcaatctgg tggggcgtcc gtccgcaatt gatgccgttg ccaatgtgct
    23101 ggggaatgcg cagcaaaaac tgcaacaggt tcagatgctg ccagtgttta ttggcatcgg
    23161 gcttggcgta ttgttaggtt ctattcccgt ctttgtgcct gggttcccgg ccgcgttgaa
    23221 actggggctg gcgggcggtc cgctgattat ggcgttgatc ctcgggcgta tcggcagtat
    23281 cggcaagctg tactggttta tgccgccaag tgccaacctc gcgctgcggg agctggggat
    23341 cgtgctgttc ctctcggtcg ttggtctgaa atctggtgga gattttgtga ataccctggt
    23401 caatggcgaa gggctaagct ggattggtta tggtgccctg atcaccgccg ttccgctgat
    23461 tactgttggc attctggcgc ggatgttagc caaaatgaat tacctgacca tgtgcgggat
    23521 gctggcaggt tccatgaccg atcctccggc gctggcgttt gctaataatc ttcatccaac
    23581 cagcggtgcg gcggcgctct cttacgccac tgtctatccg ttagtgatgt tcctgcgcat
    23641 tatcaccccc caattactgg cggtgctctt ctggagtatc ggttaactct tttccgggtg
    23701 gagtcgccgt aaatggtact ccacctggta ttcgctggca tttctgaaca tcactgaata
    23761 attcaaaaac tccccgctct cgctatatga caacgaggtg atccgcagca gtggcgtgtg
    23821 ttccgctacc ttcatttgcc gcgccagcag tctatcggcg agcactggcg tcaggctttc
    23881 ataattaccg ccaatcaaaa tcccgcactc ttgttcaata tattcaaact tcgacccttc
    23941 cagatgttgc agcgaaagat tacggaacag ttttaccggc atatagctgt cttccagcat
    24001 cagcggtttc ccttccacaa aacgaacgcg acgggagaag tagatctgct cgttgatttg
    24061 aatccgtaac tggctggcaa tggccggagg cgcaggcatg atttcaaaaa tcagcacctg
    24121 gctggtgacc gtttttccct gccgttttaa cacctccacc agcccggtca ggctggcggt
    24181 ttgatgcagt acatctttgc gcaccaggta agtgccgctg ccgtggcggc ggaccaccaa
    24241 tccccacgct accagcaggt caatggcttt acggatggtc atccgcgata ccgcaaattc
    24301 ttccgccaac tttttttcac cgggaagaag gctatttagc gtgaaatctg cggaattaag
    24361 tcgaattctt aaccgctccg caatgctttt gtagatcatc tctagatctc ttttttgcct
    24421 gcaagcacgg gtaatgcgcc cacaagttct gcttaatcga ttgaaaaaac atcttttttt
    24481 taaagatgtg ttcgatgctg tgtacctgtg ctcacagatg tctacttttt cgcgaaaacg
    24541 tagatctcta ccgcccaacg aaaagcatga aagcgatcac gaatcccatt gggtcgtcat
    24601 gttctgccga tcgcatcttc ctatcctcgc tccaggcctg ccgcataacc aatcaggctt
    24661 cctacttaca gaattgagaa aagaggatgt ggaaatgctc agtcaaattc aacgctttgg
    24721 cggcgcgatg ttcacgccag tgctgctgtt tcccttcgcc gggattgtgg tgggtcttgc
    24781 catcttgctg caaaacccga tgtttgtcgg ggaatcactg accgatccga acagtttatt
    24841 cgcgcaaatc gtacacatta ttgaagaggg cggttggacg gtgttccgta atatgccgct
    24901 gatttttgct gtcggtttac ccattggcct tgctaagcaa gcgcaggggc gtgcttgtct
    24961 ggcggtgatg gtgagtttcc tgacttggaa ctatttcatc aacgcgatgg gaatgacctg
    25021 gggaagctac ttcggcgtcg atttcactca ggacgcggtg gcaggtagcg gtctgacaat
    25081 gatggccggg attaaaaccc tcgataccag cattatcggc gcaattatca tttccggcat
    25141 tgtgacggcg ctgcataacc gtctgttcga taaaaaactg ccggtttttc tcggcatttt
    25201 ccaggggacg tcttatgtgg tgattatcgc cttcctggtg atgatcccct gtgcctggct
    25261 gacgttgctc ggctggccaa aagtacaaat ggggattgaa tctctgcaag cgttcctgcg
    25321 ttcggcgggt gcgcttgggg tgtgggttta caccttcctc gaacgtattc tgatcccaac
    25381 cggtttacac cacttcatct acggaccgtt tatctttggt ccggcagctg ttgaaggcgg
    25441 cattcagatg tactgggcgc agcatctgca agagttcagt ctgagcgccg agccgctgaa
    25501 atcgttgttc ccggaaggcg gttttgccct gcacggtaac tcaaaaatct ttggtgccgt
    25561 gggcatttct ttagcgatgt acttcactgc cgcaccggaa aatcgggtaa aagtggcggg
    25621 cttgctgatt cccgcaacct taaccgccat gctggtggga attaccgaac cgctggaatt
    25681 taccttcctg ttcatttcac cgttgctgtt tgcggtacac gccgtgctgg cggcctcaat
    25741 gtcgaccgtg atgtatctct ttggtgtggt gggcaacatg ggcggaggtc tgattgacca
    25801 ggttttaccg caaaactgga tcccgatgtt cagcaaccac gcggatatga tgctgaccca
    25861 aatcgccatt gggttgtgct ttaccctgct gtacttcgtg gttttccgca ccctgattct
    25921 gcagttcaac atgtgcacgc cgggacgtga agatgcggaa gtgaaactct actcaaaagc
    25981 cgaatacaaa gcctcgcgag gccaaaccac cgcggcagag ccaaaaaaag agctggatca
    26041 ggctgccggt attctgcaag ccctgggcgg ggtcggcaat atctccagca ttaacaattg
    26101 cgcgacgcgt ctacgcattg cactgcatga catgtcacaa acgctggatg acgaagtctt
    26161 taaaaagctg ggagcgcacg gcgtcttccg tagtggcgat gccattcagg tgatcattgg
    26221 tctgcatgta tcccaattgc gtgaacagct cgatagctta attaattctc atcaatcagc
    26281 agaaaatgtt gccattacgg aggcagtata atgaccaaat tctcagtggt tgtcgcaggc
    26341 ggtggaagca cctttacgcc aggcatcgtg ttgatgctcc tggcgaatca ggaccgtttc
    26401 ccgcttcgtg cactgaaatt ttatgataac gatggcgcgc ggcaggaagt gattgccgaa
    26461 gcctgtaaag tcatccttaa agaaaaagcg ccggacattg cgtttagtta caccaccgat
    26521 cctgaagtag cgttcagcga cgttgatttt gtcatggcgc acatccgcgt cggcaagtac
    26581 ccgatgcgcg aactggatga aaaaatccca ctgcgccacg gtgttgttgg tcaggaaact
    26641 tgcggacccg gcggaatagc gtacggcatg cgttccattg gcggcgtcct ggaactggtg
    26701 gattatatgg aaaaatattc accaaacgcc tggatgctca actactccaa cccggcagca
    26761 attgtcgcag aagccacgcg tcgtctgcgc ccgaatgcga aaatcctcaa catctgtgac
    26821 atgccaatcg gtattgaaag ccggatggcg caaattgttg ggctgcaaga tcgcaaacag
    26881 atgcgcgtgc gctactacgg cctgaaccac tggtggtcgg cgatatcccg cagtttcaga
    26941 aagggttaat gagtcagcaa gtggcggtgg aaaaactggt cgtggacgcc tgggaacagc
    27001 gttcatatca gcacctgtgg caggcgatta cgttgtcgaa aacggtaccg agcgcctcgg
    27061 tcgccaaagc tattctggat gagttgctgg aggccaacaa agcgtactgg ccagagttac
    27121 gttaattgtg tggactggca tccgttgggt gctggtccgt attctttagc acggctgacg
    27181 gcgcataacc aaatttatgt ttaaaggctt tgctgaaatg aaaggagtcg aagaagttga
    27241 gcgtatcagc gacttcgcca accgagtttc cttgttgctt taatagcgat agcgccagat
    27301 ccagacgcgc atcgaggtaa tattctttcg gcgtcttgcc ggtatagcgt aaaaacagac
    27361 gacgcaacca ggcttcgcta caggggatcg tggcagccat atcagctacg ctccagcgtt
    27421 gttgcagact ggcatgtaaa gtggcaatta atttttcaat ttgccgccgt tgtggatctt
    27481 ttttaccatc tgcgtacata agacaaatcc actgataaat aattttcgtc agaaaagcga
    27541 ctgccagatt attttttatt gcttctggtg aagttattaa ctcagcaact tcggtgagtt
    27601 cctgattata aatttcgcca ttataaataa cgctttgctg accaacggga atatccatca
    27661 tactggtggg ggtaaattcc atccagtact gttcccagac taaaccttca cagtgataag
    27721 agtgaatatc cattggcttt aagaatataa tacagttgcc atgcagtgtt atttgttcgc
    27781 cattctttaa aagaattttc ccacatcctt gtacggtata caccgcaacc catgagttgg
    27841 cgataagcgg tttcttcttg tcccgcggcc agatcacccg gtaatgctcg tcggcgtagg
    27901 tatgccagag cgagatcaga gaaattccgc tggagatcac attttcgtta atcaataagg
    27961 gaatattgta cggagtttcg ttttttacat ctgaactttg caattttcca ttcattttaa
    28021 tatccctccg gcgaattatg ctcacaacct gaacttaaca gaaaacaatc cgttcaggga
    28081 tacgacgttt gtattttaag aatctgactg cctgacccga cgcattttac ctctccctat
    28141 attcatgcgt ccgggacata aacataaata agggctatga gatgaattcg ttacaaatct
    28201 tgagttttgt cggttttacg ctgctggtgg cggtgatcac ctggtggaag gtccgcaaaa
    28261 cagataccgg atcgcaacaa ggctattttc ttgccggacg ttcactaaaa gcgccggtta
    28321 ttgccgcttc gttaatgcta accaaccttt ccacggaaca actggtcggc ctttccgggc
    28381 aggcctacaa aagcggcatg tcggtgatgg gctgggaagt gacttcagcg gtgacgctga
    28441 tcttcctcgc gctaatcttt ttaccgcgct atctgaagcg cggcattgcc accatccccg
    28501 attttctgga ggaacgttat gataaaacga cgcgtattat catcgacttc tgcttcctca
    28561 ttgccaccgg cgtctgcttt ctgccgattg ttctctactc cggcgcgttg gcgctcaaca
    28621 gcctgtttca cgtcggggaa tcgctacaga tttctcacgg tgcggctatc tggctactgg
    28681 taattttgct tggtctggcg ggaattttgt atgcggtgat cggcggactg cgcgcaatgg
    28741 cagtggcgga ctccatcaac ggtattgggc tggttatcgg cgggttgatg gtgccggtat
    28801 ttggcctgat cgcgatgggc aagggcagct ttatgcaggg cattgagcaa ctcaccaccg
    28861 ttcacgccga gaaattaaac tcaatcggtg gcccgaccga tcccttgccg attggcgcgg
    28921 catttaccgg tttgattctg gtgaacacct tttactggtg tacaaatcag ggcatcgtgc
    28981 aacgcacgct ggcgtcaaaa agcctggcgg aagggcaaaa gggggcgctg ttaacggcgg
    29041 tgctgaaaat gctcgacccg ctggtactgg tgctgccagg gttgattgcg tttcatctgt
    29101 atcaggattt accgaaagcc gacatggcct acccgacgct ggtcaataac gttctgccag
    29161 tgccaatggt gggtttcttc ggcgcggtgt tatttggtgc ggtgatcagt accttcaacg
    29221 gctttctgaa tagcgccagt acgttattca gtatgggtat ttaccgtcgc atcattaacc
    29281 agaatgccga gccgcagcag ctggtcaccg tcgggcgcaa atttggtttc tttatcgcta
    29341 tcgtttcggt gctggtcgcg ccgtggatcg ccaacgcgcc gcaggggctg tatagctgga
    29401 tgaaacagct caacggcatt tacaacgtgc cgctggttac catcatcatt atgggctttt
    29461 tcttcccgcg catcccggcg ctggcggcaa aagtggcgat ggggattggc ataatcagct
    29521 acatcaccat caactatctg gtgaagttcg acttccattt cctctatgtg ctggcctgta
    29581 cgttctgcat caacgtggtc gtgatgctgg tgatcggttt tatcaaaccg cgcgccacgc
    29641 cgttcacctt caaagatgcg tttgcggtgg acatgaaacc gtggagaaac gtcaagatcg
    29701 cgtcaattgg catcctgttc gcgatgattg gcgtctatgc cgggctggct gaattcggcg
    29761 gctacggtac gcgctggtta gcgatgatca gttatttcat tgccgccgta gtgattgtct
    29821 acctgatttt tgacagctgg cggcatcgtc acgacccagc cgtaaccttt actcccgacg
    29881 cgaaggatag cctatgaaac gccccaattt tctgttcatc atgaccgata cccaggccac
    29941 caatatggtc ggttgctata gcggtaaacc gctgaatacg caaaatattg atagtctggc
    30001 ggcggaaggt attcgcttta attccgccta cacctgttca ccggtttgta cgcctgcacg
    30061 cgccggacta tttaccggta tctacgctaa ccagtccggc ccgtggacca acaacgtcgc
    30121 gccaggcaaa aacatctcca ctatggggcg ctactttaag gatgccggct atcacacctg
    30181 ttacatcggc aaatggcatc tcgacggtca tgactatttc ggcactggcg agtgtccgcc
    30241 ggagtgggac gctgattact ggttcgatgg ggcgaactac cttagcgaac tgacggaaaa
    30301 agagatcagc ctgtggcgca atggcctaaa cagcgttgag gatttacagg cgaaccatat
    30361 cgacgaaacc ttcacctggg cgcaccgcat cagcaatcgg gcagtagatt ttctgcaaca
    30421 gcccgcgcgc gccgaggaac ccttcctgat ggtggtttcg tatgatgagc cgcatcaccc
    30481 gttcacctgt ccggtggagt atttagagaa atacgctgat ttttactacg atctgggcga
    30541 gaaagctcag gatggcctgg cgaacaaacc ggaacatcac cgcttatggg cgcaggcgat
    30601 gccatcgcca gtcggtgatg acgggcttta tcaccatccg ctctattttg cctgtaatga
    30661 ctttgttgat gaccaaatcg gacgggtcat caatgcctta acgccagagc aacgtgaaaa
    30721 tacgtgggtc atttatactt ccgatcacgg cgaaatgatg ggcgcacata agctgatcag
    30781 taaaggagcg gcgatgtatg acgacatcac ccgtattccg ctgatcatcc gttcgccgca
    30841 aggggagcgg cgacaggtcg atacgccagt cagtcatatc gatttactgc cgacaatgat
    30901 ggcgctggca gatattgaaa aaccagagat tctgccgggg gaaaatatcc ttgccgtgaa
    30961 agagccacgc ggtgtaatgg tggaatttaa ccgctacgag attgagcatg acagctttgg
    31021 cggttttatt ccggtgcgtt gctgggtgac ggatgacttt aaactggtac tcaacctctt
    31081 caccagtgat gaactttacg atcgccgtaa tgacccaaat gaaatgcata acctgatcga
    31141 tgatatccgt tttgcagacg ttcgcagcaa aatgcatgac gccttattgg attacatgga
    31201 caaaattcgc gatccgttcc gcagttacca atggagcctg cgtccgtggc gtaaagatgc
    31261 actgccgcgc tggatggggg catttcgtcc acgcccacaa gatggctatt cgccggtggt
    31321 acgtgactat gacaccggcc taccgacgca aggagtgaaa gtggaagaga aaaaacagaa
    31381 gttctgacga taatgcccgc tgatgaacac ccggcgggca ttattcaggc tgcttaaacc
    31441 agtgaaccgc cggtcaggaa ggaaaaaatc aggaatatca gcaaaatgct gcacacgatg
    31501 ccgataccaa aaagcaaggt atggaaagcc agcgtcccgc cgccaaaaac cagcgcgccg
    31561 cgaatgcccc agcgattttc cttacgcata ccgccaataa atgcgcaaag tagcgcagca
    31621 atggccagtg cgataccgct ttgatcgagg attttatcga catctacgtc ctcttttttc
    31681 tccacggtgg taattttttc gccttttatt ccggcgagaa gacctctttt aatttcagcc
    31741 gttttgtccg ccacgatgct ttcaaacgtt ggcggtggtg cggaaaaagg gccgaatgaa
    31801 aagtggataa tccccatcat taaagcgatg gcaccgaaca gcatgcccaa tgaactgatt
    31861 ttattttgag ctatgatacc catctgacct ccttgtagcg gatggcgaaa gcatatcagg
    31921 aaacgggcgg acgaaattct taaaaaaaaa ccgctaaagg tagagcgtta gtgaaattgc
    31981 aaaggagcaa caatgaaaat ttcccgcctt ggagaagcgc ccgattaccg cttctcgctg
    32041 gctaatgagc gtacttttct ggcgtggatc cgtaccgcac taggatttct ggcggcgggg
    32101 gttgggcttg atcaacttgc gccagatttc gccacgcccg tcattcgtga actgctggcg
    32161 ttgttgttgt gtctgttttc cggcgggtta gcgatgtatg gctacctgcg ctggttacgc
    32221 aatgaaaaag cgatgcgtct gaaagaggac ctgccctaca ccaacagctt attaatcatt
    32281 agcttaatat tgatggtcgt ggcggtgatc gttatgggac tggtgttgta tgccggatag
    32341 ccgcaaagcc agacgcattg ccgacccagg actgcagccg gagcgcacat cactggcgtg
    32401 gtttcgtacc atgctgggct acggcgcgtt gatggcgttg gctatcaaac acaactggca
    32461 ccaggcgggc atgttattct ggatttcgat tggcattctc gccatcgtgg cgctgatcct
    32521 ctggcactac acccgtaatc gcaatttaat ggatgtcacg aatagcgatt tttcccaatt
    32581 tcacgtagtc cgtgacaaat ttttgatctc cctcgcggtg ttatctctcg caatactgtt
    32641 tgctgtaacg catatacatc aacttatcgt atttattgag agagtcgcat gacaggaagt
    32701 caggtcattg atgctgaaga ggacaggcat aaactggttg tagagtacaa ggatgctctg
    32761 caacctgccg atttttatca caatttcaaa cagcggggca tccgctccgt acagctcatt
    32821 ccctatcttg aatttgatga ccggggcgat ctgacggccg cttcggtgac agcagagctg
    32881 tgggggaaat ttttaattgc cctgttcgag tgttgggtgc gggcagatat cagtcgtatc
    32941 tcgattgagc tttttgacgc caccttacaa aaatggtgtg gcagcgaaaa cccgcaaccg
    33001 cgccgtgact gccaggcgtg cgactggcat cgcctgtgcc cgcacgctcg ccaggagacg
    33061 ccagacagcg tgctttgtgc gggctatcag gcgttttatt cttactccgc accgcatatg
    33121 cgggtgatgc gtgatctgat aaagcagcat cgttctccca tggagctgat gacgatgctg
    33181 cggtgacgtg cgcttaaacg ggctgcccct gatgcgacat ccgcgtcgcc agcggcagcc
    33241 agcacagcac gatcaacaat cccattaagg tcatcaacaa ccccaggctg ccctgaccgg
    33301 tttgcggcaa catcgcagag agcgacgcca gcacgccgga accaatgttt tgcagaccgc
    33361 cgaccagcgc gccagccgtg cccgccagga aggggaacgg ctccatcgcg ccgctggtcg
    33421 ccagcggaaa cagcatcccg gcaccgaaaa agaacagcgc ggcgggaacg agcagcgtcc
    33481 agacattcat cacgccaaac cagtcgggga tccacatcag caagccagcc agcaggcagc
    33541 agataaccga ctgccacatt aacgtggaga agcgtttatt gggacgtccg gcaaaccatg
    33601 cgccaaaaaa cgctgccgga atcggcagaa taaacaaaat actgaccgtc atactgctca
    33661 gccctaacac cgcgcccatc agcacgccgg agcaggcttc aaaggcggca atcccggcca
    33721 gaccgccaat cagcatcagc aaataacagt taaaaccgct gttaccgaaa agcgttttat
    33781 aactggtaag caggcgcgtg cgcggtgcat cgaccggacg cgtttccggc atccagcggg
    33841 ccatactgaa ggtcacacca gcacaaagaa ccaacaaaaa gagataacag gcgcgccagt
    33901 tccacatcgt atccagcaga ccgccgatta gcggtgcgag caacggactg acgagaatcc
    33961 ccatgtttaa caggctgtta gcatggcgca actgtgtccg ttcatataaa tctcgcggca
    34021 aagtacgcgc cattacgccg ccaacgccgg tgcccatccc ctgcatcgcg ctggcggcaa
    34081 tcaacaccgt caaactggag gtcgtgaccg cgaccagcgt tgccagcata aaaatggaca
    34141 ttccgacgag gatcaccggt cggcggccca cgcggtcgga aatcgggcca taaaacagct
    34201 gtgagacacc gtaagtcagc agataagcgc ccattacgct ctgcaccgcc ccttcacgga
    34261 cgttgagatc gcgcgccata tcggcaatag ctggaatata aatggtttgc gccatctgac
    34321 cgacggccac gagtaatacc aacatcaata acaaattgac gtttctttgc cttttcatta
    34381 tcacggatgc ttttataaaa ataattagaa taagtcacta cgcattccca taaatgcggg
    34441 agaggaatct atcacattga tgaatgttag ccagatatac gccaggaatg gggaattgtt
    34501 tagcggcaga atatgtaaac aaaagcggca ataaatgttg ccgggaggga ttacgtgcga
    34561 actgtaaccc tctccccaaa ggggcgaggg ggaaaccacc gttccctttc agaaaatgag
    34621 ctaaacattg cacgggacgg tcccctcgcc cctttgggga gagggctagg gtgaggggca
    34681 caggcagcgc aggtgcaaac attctgctgg tagaagaagc ccccaactcc aacccgcacg
    34741 ctaaatataa aaggggagcg gttacccgct cccctttggt gcgacttgaa tctgaattac
    34801 ttcaggtatt tcagaacagc atcaagcagt tgcagtgctg caacaatgag tttgaggata
    34861 agaatggcga tatccaccag gctcatacgc gtctcctgtg gttcaggagc actgccagct
    34921 gacgtacctt tccgctgcct gttgccagct gttgtgcacg cggtatctta aggagaaacg
    34981 ttgtgcttgt tgacataaca cagtgtgctc acgcggggtt aaggcgctga cggcaccacc
    35041 cgtttcagcc aggacttagt gcgccgggta acgatgcggc ccccgcataa cacattgcgt
    35101 acagtgatat tatagaagtt tactgtataa ataaacagta atatttggac aaaacgcaaa
    35161 ctgtgatcga tactcaacta tgccctaaat acgtcaaaat tcgtgccgaa attgcgcgtt
    35221 ctgcgcggaa cacgtatact ttcagtgttg acataataca gtgtgctttg cggttaccag
    35281 ccgcaggcgg ctgatgaaac ctcgctccgg cggggttttt tgttacctgc aattcagtac
    35341 aaaacgtgat cagcccctca attttccctt gctgaaaaat tttccattgt ctcccctgta
    35401 aagctgtgct tgtataaata ttgttaaaca caaaaccaac aaggtcccca atgactactt
    35461 ccatgttcaa cgcaaaacta ctaccaactg cgctatccgc cgcagtggtc gtcgtgcgtg
    35521 tggtggtggt cgtcggcaat gcgccgtagg gactggaaca acacacgatt ccaaaacccc
    35581 gccggcgcaa accgggcggg gtttttcgtt taagcacctc ccggaaagtc ggcccagaag
    35641 aaaaggactg gagcatggca agttcgggca caacatcgac gcgtaagcgc tttaccggcg
    35701 cagaatttat cgttcatttc ctggaacagc agggcattaa gattgtgacg ggcattccgg
    35761 gcggttctat cctgcctgtt tacgatgcct taagccaaag cacgcaaatc cgccatattc
    35821 tggcccgtca tgaacagggc gcgggattta tcgctcaggg gatggcgcgc accgacggta
    35881 aaccggcggt ctgtatggcc tgtagcggac cgggtgcgac taacctggtg accgccattg
    35941 ccgatgcgcg gctggactcc atcccgctga tttgcatcac cggtcaggtt cccgcctcga
    36001 tgatcggcac cgacgtcttc caggaagtgg acacctacgg catctctatc cccatcacca
    36061 aacacaacta tctggtcaga catatcgaag aactcccgca ggtcatgagc gatgccttcc
    36121 gcattgcgca atcaggccgc ccaggcccgg tgtggataga cattcctaag gatgtgcaaa
    36181 cggcagtttt tgagattgaa acacagcccg ctatggcaga aaaagccgcc gcccccgcct
    36241 ttagcgaaga aagcattcgt gacgcagcgg cgatgattaa cgctgccaaa cgcccggtgc
    36301 tttatctggg cggcggtgtg atcaatgcgc ccgcacgggt gcgtgaactg gcggagaaag
    36361 cgcaactgcc taccaccatg actttaatgg cgctgggcat gttgccaaaa gcgcatccgt
    36421 tgtcgctggg tatgctgggg atgcacggcg tgcgcagcac caactatatt ttgcaggagg
    36481 cggatttgtt gatagtgctc ggtgcgcgtt ttgatgaccg ggcgattggc aaaaccgagc
    36541 agttctgtcc gaatgccaaa attattcatg tcgatatcga tcgcgcagag ctgggtaaaa
    36601 tcaagcagcc gcatgtggcg attcaggcgg atgttgatga cgtgctggcg caattgatcc
    36661 cgcaggtgga agcgcaaccg cgtgcagagt ggcaccagtt ggtagcggat ttgcagcgcg
    36721 aattcccgtg tccaatcccg aaagcgtgcg atccattaag ccattacggc ctgatcaacg
    36781 ccgttgccgc ctgtgtcgat gacaatgcga ttatcaccac cgatgtgggt cagcatcaga
    36841 tgtggaccgc gcaagcttat ccgctcaatc gcccacgcca gtggctgacc tccggtgggc
    36901 tgggcacgat gggttttggc ctgcctgcgg cgattggcgc ggcgctggcg aacccggatc
    36961 gcaaagtgtt gtgtttctcc ggcgacggca gcctgatgat gaatattcag gagatggcga
    37021 ccgccagtga aaatcagctg gatgtcaaaa tcattctgat gaacaacgaa gcgctggggc
    37081 tggtgcatca gcaacagagt ctgttctacg agcaaggcgt ttttgccgcc acctatccgg
    37141 gaaaaatcaa ctttatgcag attgccgccg gattcggcct cgaaacctgt gatttgaata
    37201 acgaagccga tccgcaggct gcattgcagg aaatcatcaa tcgccctggc ccggcgctga
    37261 tccatgtgcg cattgatgcc gaagaaaaag tttacccgat ggtgccgcca ggtgcggcga
    37321 atactgaaat ggtgggggaa taagccatgc aaaacacaac tcatgacaac gtgattctgg
    37381 agctcaccgt tcgcaaccat ccgggcgtaa tgacccacgt ttgtggcctt tttgcccgcc
    37441 gcgcttttaa cgttgaaggc attctttgtc tgccgattca ggacagcgac aaaagccata
    37501 tctggctact ggtcaatgac gaccagcgtc tggagcagat gataagccaa atcgataagc
    37561 tggaagatgt ggtgaaagtg cagcgtaatc agtccgatcc gactatgttt aacaagattg
    37621 cggtgttttt tcagtaaccg ctcaaggctt gaacaacatc gcgcttatcg ttaaggtaag
    37681 cgcgtttttt tttacccgcc aggacaagac catgatcacc gttgccctta tagacgatca
    37741 cctcatcgtc cgctccggct ttgcgcagct gctggggctg gaacctgatt tgcaggtagt
    37801 tgccgagttt ggttcggggc gcgaggcgct ggcggggctg ccggggcgcg gtgtgcaggt
    37861 gtgtatttgc gatatctcca tgcccgatat ctccggtctg gaactgctaa gccagctgcc
    37921 gaaaggtatg gcgacaataa tgctctctgt tcatgacagt ccggcgctgg ttgagcaggc
    37981 gcttaacgcg ggggcacgcg gctttctctc caaacgctgt agccctgacg aactgattgc
    38041 ggcggtgcat acggttgcca cgggcggctg ttatctgacg ccggatattg ccattaaact
    38101 ggcatccggt cgtcaggacc cgctaaccaa acgcgaacgg caggtggcgg aaaaactggc
    38161 gcaaggaatg gcggtgaaag agattgctgc cgaactgggc ttgtcgccga aaacggtgca
    38221 cgtccatcgc gccaacctga tggaaaaact gggtgtcagt aacgacgtag agctggcgcg
    38281 ccgcatgttt gatggctggt gatgaagacg ttgttctccc gcttaattac cgttattgcc
    38341 tgctttttta tcttctctgc cgcatggttt tgcctgtgga gtatcagcct gcacctggtt
    38401 gagcgccctg atatggcggt gctgttattt ccgtttggtc tgcgtctggg gctaatgctg
    38461 caatgcccgc gcggatactg gccggtgctg ctgggcgcgg agtggctgct gatttactgg
    38521 ctaacgcagg cggtcggttt aacccatttt ccgttattga tgatcggtag tttactgacg
    38581 ttactgcccg tggcgctgat ctcgcgctat cgccatcagc gtgactggcg caccttgctg
    38641 ttacagggtg cggcgttaac ggcggcggcg ttgttgcagt cgctgccctg gctttggcac
    38701 ggcaaagagt cgtggaatgc gctgttgctg actttaactg gcggcctgac gctggccccg
    38761 atatgtctgg tgttctggca ctatctcgcc aataacacct ggctgccgct cggcccgtca
    38821 ctggtttctc agccgatcaa ctggcgcggg cgacatctgg tctggtactt gctgctgttt
    38881 gttatcagtc tctggctcca gttgggattg ccggacgaac tgtcgcgctt tacgccattt
    38941 tgtctggcgc tgccgattat cgcgctggcc tggcactatg gttggcaagg ggcgctgatt
    39001 gcgacgttga tgaacgccat cgcgctgatc gccagtcaaa cctggcgcga tcatccggtg
    39061 gatttattgc tctcgctgct ggtgcaaagt ctgacagggt tgttgcttgg cgctggcatc
    39121 cagcggttgc gtgaacttaa ccagtcgctg caaaaggaac tggcgcgcaa tcagcatctg
    39181 gctgaacggt tgctggaaac cgaagagagc gtgcgccgtg atgtggcgcg tgagctgcat
    39241 gatgatatcg gtcagaccat cacagccatt cgtactcagg cgggcattgt tcagcggctg
    39301 gcggcagata acgccagcgt gaagcagagc gggcagctca tcgaacaact atcgctgggc
    39361 gtttacgacg cggtgcgccg tttgttgggt cggttacgtc cgcgccagtt ggatgatctc
    39421 accctggagc aggccatccg ctcactgatg cgggaaatgg agctggaagg gcgcggtatt
    39481 gtcagccatc tcgaatggcg aatcgatgaa tcagcgttaa gcgaaaacca gcgcgtgacg
    39541 ctgtttcgtg tctgccagga agggctgaac aacattgtga aacatgctga tgccagcgcg
    39601 gtcaccctgc aaggctggca gcaggatgaa cggttgatgc tggttattga agacgatggc
    39661 agcggtttgc cgccgggttc cgggcaacaa ggttttggcc tcaccggaat gcgcgagcgc
    39721 gtaacggcgc tgggtggcac attacacatt tcctgtctgc acggcacgcg tgtcagcgtt
    39781 tctctacctc aacgctatgt ctaaggtttg atgatgttgc cgtttctgaa agcgcctgcc
    39841 gatgcgccat taatgactga taaatatgaa attgatgccc gctatcgcta ctggcgtcgg
    39901 catattctgc tgaccatctg gctgggttac gcgctgtttt acttcacgcg gaaaagtttt
    39961 aacgccgcgg taccagaaat ccttgctaac ggcgtgctca gccgtagcga tatcggcctg
    40021 ttagcgaccc tgttttacat tacttacggc gtgtcgaagt ttgtctccgg cattgtcagc
    40081 gatcgctcaa atgcccgtta ttttatgggg atagggctta tcgccacggg catcatgaac
    40141 attctgtttg gcttctcgac gtcgctatgg gcgtttgccg tgctctgggt gctgaacgcc
    40201 tttttccagg gctggggttc accggtgtgt gcgcgtctgt taacggcctg gtattcacgt
    40261 accgagcgcg gcggttggtg ggcattatgg aacacggcgc ataacgtcgg cggcgcactc
    40321 attcccattg tgatggcagc gagcgcgctg cattacggct ggcgtgccgg gatgatgatt
    40381 gctggttgta tggcgatagt cgtggggatt tttctctgct ggcggctacg cgatcgcccg
    40441 caggcgttag gtttaccggc ggtcggtgaa tggcggcacg acgcgctgga aattgctcaa
    40501 caacaagaag gggcagggtt gacgcgtaaa gagatcctga ccaaatatgt gttgctgaat
    40561 ccgtatatct ggctgctttc gttttgctat gtgctggtct atgtggtccg ggcggcaatc
    40621 aacgactggg gcaatttgta tatgtccgag acgctgggcg tcgatctggt cacggcgaat
    40681 acggcagtga cgatgtttga actgggcgga tttatcggtg cgctggtagc cggttggggc
    40741 tcggacaaat tgtttaacgg caaccgtggg ccgatgaatt tgattttcgc cgccggaatt
    40801 ttgctttcag tcggctcgct gtggctgatg ccatttgcca gctacgtgat gcaggcaacc
    40861 tgcttcttca ccattggttt ttttgtcttt ggcccgcaga tgttaatcgg catggcggcg
    40921 gcagagtgtt cccacaaaga ggctgcaggg gcggcgacgg ggtttgtcgg cttgtttgct
    40981 tatctggggg cgtcgcttgc tggttggccg ctggcgaaag tactcgatac ctggcactgg
    41041 agcggatttt ttgtggttat ctctatcgcc gccggggttt ccgcactgct gttactgccc
    41101 tttttgaacg cccaggcacc gcgcgaagcg tgatgcatct caccttttca cttcatatcc
    41161 ggcaaaacta agaaattttc caggttttgc ctggacgcta tctcaggcct gatttgctgc
    41221 tgatttttac aatgccatca ttcattaatt ctttcccaaa ataattcaag ttgcaggaac
    41281 gcagcaaacg cgtgagtccc gggagcatag tgaactatgt gaccgggagg aatgcgtgca
    41341 gctaaggcac ctgcggcttg aagtatgaag ggaaatcagg agtaacccat gctggctttc
    41401 ttaaaccagg ttcgcaagcc gaccctggac cttccgctcg aagtgcggcg caaaatgtgg
    41461 ttcaaaccgt tcatgcaatc ctacctggtg gtctttatcg gctacctgac gatgtacctg
    41521 attcgcaaga actttaacat cgcgcagaac gacatgatct cgacctacgg gttgagcatg
    41581 acgcagctgg ggatgatcgg cctgggtttc tccatcactt atggcgtggg taaaacgctg
    41641 gtttcctact acgccgacgg caaaaacacc aaacaattcc tgccgttcat gctgatcctg
    41701 tctgccattt gtatgctggg cttcagtgcc agtatgggca gcggctcggt tagcctgttc
    41761 ctgatgattg ccttctacgc cttaagcggc tttttccaga gtaccggcgg ttcgtgcagt
    41821 tactccacca tcaccaaatg gacgccgcgt cgtaaacgcg ggacattcct cggtttctgg
    41881 aatatttctc acaaccttgg cggtgcaggc gcagcaggtg tggcgctgtt cggggcaaat
    41941 tacctgttcg atggccatgt catcggcatg tttatcttcc cgtcgattat cgcgctgatt
    42001 gtcggtttta tcggcctgcg ttacggcagc gactccccgg aatcttatgg cctcggcaaa
    42061 gctgaagaac tgttcggcga ggagatcagc gaagaggaca aagagacaga atctaccgat
    42121 atgaccaagt ggcagatctt tgttgagtat gtgctgaaaa acaaagtgat ctggctgctg
    42181 tgcttcgcca acattttcct ctatgtggta cgtattggta tcgaccagtg gtcaaccgta
    42241 tacgcgttcc aggaactgaa actctctaaa gcggtggcga ttcagggctt tacgctgttt
    42301 gaagctggtg cactggtcgg tacgctgctg tggggctggc tctctgacct ggcgaacggt
    42361 cgccgtggcc tggtggcctg catcgcgctg gcgctgatta tcgccacgct cggtgtgtat
    42421 caacatgcca gcaacgaata tatctatctg gcttctctct ttgcgttggg tttcctggtc
    42481 tttggcccgc aattgttgat tggtgtggct gctgttggct ttgtacctaa aaaagcgatt
    42541 ggcgctgccg atggtattaa aggcaccttt gcttacctga ttggtgacag ctttgccaag
    42601 ttaggtctgg gaatgattgc cgatgggacg ccggtattcg gccttaccgg ctgggcaggc
    42661 accttcgccg cacttgatat cgccgcgatt ggttgtatct gcctgatggc gatagtggcg
    42721 gtgatggaag aacgcaaaat ccgccgcgag aaaaaaattc agcagttgac agtggcataa
    42781 acgtaactgg tgacttttgc ccggcatgac gccgggcttt tttattattc cgtgacttcc
    42841 agcgtagtga aggcaaactt ctcgccatca aatagcccct gactggttag ttttagcgcg
    42901 gggatcactg gcagagaaag aaacgccatc tgaataaacg gctcatcggg taacggaccg
    42961 cattcacggg cggcggcttt caaggcgtca atttgttccg ccagcgactg cgccgtgtcg
    43021 gtgctcatca ggccggcaat gggcaacggc agatgacttt ggacctggcc gttacgtacc
    43081 acgcacagcc cgccgccatc ctgaatcacc tgattgaccg ccagcgccat ctcttcggca
    43141 ctgcgaccga tcaccacaat attatggctg tcatggctga ccgtcgcagc cagcgcacct
    43201 tcattcagtc caaagccgcc aagcaaacca caagccggag ccagccgttg cccgtaacgc
    43261 tcaagtacgg caataaagct cacatcatcg cgatcaaaac cattttcgct gtagacgctg
    43321 gagtgggagt gcgtaatcaa ttcgttatgg atgacgtcaa tgacccgata gcgttttccg
    43381 ggcgtaaatt gcagggcaaa gtcgctggcg gaaactggct ggcgggcaat ggtgttgcca
    43441 tatggcggag cggattgtgc cagtctcgcc gactcttccg cctgtaaggt ttgcgcatca
    43501 atcggctcgc ctttcaccag tacctgctgc accgtgacct tacgcgcatc gctcaacagg
    43561 acgatatcgg cctgcttgcc gggtgccagt aagccgaggt gattcagacc aaagtggcgc
    43621 gccgtcgacc agctggcgac gcgatatgcc acatgcagcg gcacattgtg ttgttcgatc
    43681 aggcggcgaa ttaaggcatc gatgtgtcct tcatgggcga tctcccacgg gttacggtca
    43741 tcggtacaga gcatgcattg cgggctgtta aattcgttga tcaacggtgc cagcgcgttg
    43801 agattgcggg cagcggaccc ttcgcggatc atcaacgaca tgccgagttg taatttccgg
    43861 cgtccttctt ccagctgata actttcgtgg cagttttcaa tacccgcagt aatataggcg
    43921 ttaagttctt taccacccaa acccgggcag tgaccgtcca gcgtcaggtg gcgaaatgca
    43981 tccagtttat cgagcagcgc attctgcccg ctaattacgc cagggtagtc catcatttct
    44041 gcaaggccgg taacctgcgg atggtcccgc caggcgagca tctgttcaag ggtaaaactg
    44101 gcaccgttaa catcgcagcc ttccagggcg ggtacgcaag agctgacctg taagtactgg
    44161 ttttgccttg cctgttcggc acagcgggca aaccaggcga atccggcttc tcccatcacg
    44221 ttgacgattt catgagggtc gcaaataacg gtcgtcaggc cgcgcggcag ggtagcggtt
    44281 tcaaaagtga ccggcgtcat catgctggat tcaatatgca ggtgagcatc aataaaccct
    44341 ggcaccgccg ttgcgccgcg agcatcaatc cgctgcaaag ccggagcatc agcgtattct
    44401 gcgccaacac cggcaatgta acgtccttta atcacaattg ggccggaaat ttctccgcca
    44461 ttgatcaggt cgagaataga gacattatca ataatataat cggcaacagc gtcgccacgg
    44521 gaaacggcta acaattcctg gtattcagcc cggctaatgt gatgaaattt atggttaata
    44581 gaattattca tatttttctc attttgattt tatcaatttg attattattt atcataaaga
    44641 aacgtcgcga gattattaaa gaatatccat taatgtgcaa ttgaaatgtg ataattatca
    44701 ctgaaccgtt gttgtcattt gttttacaaa agcaagggat taaatctcct ccaatggaca
    44761 aaaaaatgaa taatgacaat accgattacg tgagtaatga atcagggacg ctttcgcgat
    44821 tatttaaact acctcagcat gggaccaccg tccgcacaga attgattgcg gggatgacca
    44881 cttttttaac catggtgtac atcgtttttg tgaacccgca aatcctcggc gcggcacaaa
    44941 tggacccgaa agtggtgttt gttaccacct gtttgattgc cggtatcggc agtattgcga
    45001 tggggatatt tgctaactta cccgtggcgc tggctccggc aatggggctg aacgccttct
    45061 ttgccttcgt ggtcgtgggg gcgatgggca tctcctggca gaccgggatg ggcgcaatat
    45121 tctggggcgc agttggacta tttttgctca cgctgtttcg tatccggtac tggatgatct
    45181 ccaacattcc cttaagttta cgtattggta tcaccagcgg aattggatta tttatcgcct
    45241 taatgggatt aaaaaatact ggcgttattg tcgccaataa agacacgctg gtgatgattg
    45301 gcgatttaag ttctcacggc gtgttgttag gtattttagg gttttttatt ataaccgtgt
    45361 tgtcatcacg tcattttcat gccgcggtgc tggtttctat tgtggtgacg tcttgctgtg
    45421 gattattttt cggtgatgtt cattttagcg gcgtctattc cattccgcct gatattagcg
    45481 gcgtcattgg tgaagtagat ttgagcggcg cgttaacact tgaactcgcc ggtatcattt
    45541 tctcctttat gctgatcaac ctatttgatt catcaggaac attaattggt gtaactgata
    45601 aagcgggctt aatagatagt aacggtaaat tccccaatat gaataaggcg ctgtatgttg
    45661 atagcgtcag ttcggtggcg ggggcgttta tcggcacctc gtctgttact gcctatattg
    45721 aaagtacttc tggtgtggca gtcggtggtc gcacggggct gactgcggtt gtggttggcg
    45781 ttatgttcct gttagttatg ttcttctcac cgctggtggc gatggttcct ccttacgcaa
    45841 ccgccggagc gttaatcttt gttggcgtgc tgatgacttc gagcctggcg cgcgttaact
    45901 gggatgattt taccgaatcg gtgcctgcgt ttattaccac ggtgatgatg ccctttactt
    45961 tctcgatcac cgaagggatt gcactcggct ttatgtcgta ctgcatcatg aaagtatgca
    46021 ccgggcgctg gcgcgatctg aacctgtgtg tggtggtggt cgcagctctg tttgcactga
    46081 agattattct ggtggattag taactggcga gggtttccag atatcatgag ttctgattac
    46141 gcaggagaac tcatgatctg gataatgctc gccacgctgg cggtagtgtt tgtggttggt
    46201 tttcgggtgc tgacatccgg ggccagaaaa gcgattcgcc gtctcagcga tcggctgaac
    46261 atcgatgtcg tacccgtgga gtcgatggtc gatcaaatgg gaaagtcagc tggtgacgaa
    46321 tttttacgtt atttgcatcg tccggatgag tcgcacctgc aaaacgccgc gcaggtgttg
    46381 ctcatctggc aaattgtcat tgtcgatggt agcgaacaga acctgctgca atggcatcgg
    46441 attttacaaa aagctcgcct cgccgcgccg attaccgacg ctcaggtcag gctggcgcta
    46501 ggttttctgc gcgaaaccga acctgaaatg caggatatta atgcttttca gatgcgctat
    46561 aacgcgttct ttcagcctgc cgagggcgtt cactggttgc attgaaaccg tttcgtctta
    46621 tcagcctaca gaccgcgcct gacctgtcgg gtggataaga cgttcacgcc gcatccggta
    46681 atctttgccc tgttccgttt cgatcttaac tccagctcaa gcaacaccgt aaaagttgca
    46741 aaaacgttaa atgcgtcaca catttcaatg ttaaagtggt cggcttttcc cctgaaacat
    46801 gccacgggta acaccatgag tgaatttatt gccgaaaacc gcggcgcgga tgccatcacc
    46861 cgaccgaact ggtcagccgt tttctcggtg gcgttttgtg tcgcctgtct gattatcgtt
    46921 gagtttttgc ccgtcagttt gttgacgcca atggcccagg atttaggcat ttcggaaggg
    46981 gttgccgggc aatcggtgac cgtgaccgcc tttgtggcaa tgtttgccag tttgtttatt
    47041 acccagacaa ttcaggctac tgaccgccgc tacgttgtta ttttgtttgc cgttttgctg
    47101 acgctctcct gcttgctggt ttcctttgct aactcattca gtttgctttt aatcggtcgt
    47161 gcctgtctgg ggctggcgct gggcgggttc tgggcgatgt cggcgtcgct gaccatgcgt
    47221 ctggtgccgc cgcgtacggt gccgaaggcg ctgtcggtga tcttcggcgc ggtttctatt
    47281 gcgctggtga ttgccgcgcc gttgggcagt tttttaggcg agcttatcgg ttggcgcaat
    47341 gtctttaatg cggcggcggt gatgggcgtg ctgtgtattt tctggattat caaatcattg
    47401 ccttcactgc caggcgaacc ctcgcatcag aaacaaaata ctttccgctt attacaacgt
    47461 ccgggtgtga tggcagggat gatcgccatc ttcatgtctt tcgccgggca gtttgctttc
    47521 ttcacatata ttcgcccggt gtatatgaac ctggcgggat tcagcgtgga tggcttaacg
    47581 ctggtgctgt tgagttttgg cattgccagc tttatcggta cgtcgctttc gtcattcatt
    47641 cttaaacgtt cggtaaaact ggccttagca ggcgcgccgt taatactggc tgtgagtgcg
    47701 ttggtgctga cgttgtgggg aagcgataaa atcgttgcta ccggcgtggc gattatctgg
    47761 gggctaactt ttgcattggt tcccgtcggc tggtcaacgt ggatcacccg ctcgctggcc
    47821 gatcaggcag aaaaagccgg gtctattcag gtggcggtta ttcagcttgc taatacctgt
    47881 ggcgcggcaa tcggcggtta tgcgctggat aatattggtc tgacttcgcc gttgatgttg
    47941 tccggcacat tgatgttgct gactgcattg ttggttactg caaaggtgaa aatgaagaaa
    48001 tcctgatatc aggtaaagaa gcgttggtca gtagttgttg ctgttcttac atatccgggc
    48061 attctacctt acccaacgct tcccaataat tgctctgatt attacgttcc atcgccacga
    48121 tggcctcgtg tatatgttgt ttattgtttt catctgacaa cattaacttt tcccacgctt
    48181 catctgataa tgacgcctgt ggcgtgcaaa ttttacgtaa atcttgtaat accgtggttt
    48241 tctcttttgc ggactgcagt gagctaaaag gcgactcggc gtatacgatg cctggcatag
    48301 cgaaaaaagt gaaaataagc agtagtgtcg ttggtttcat ggattcaacc ttttacaaat
    48361 gtgtatcgaa tgaagcataa gtgtagtttg ctttcatcgc tctcatcatg ctgtacgaaa
    48421 cgcgcttgcg tggtcagtaa gaagtgccag actttatatt ccacttttat tcctttttat
    48481 tcttatcgat agcgtttcgt tttttaaacc gcagcgacct taccgctata gtcaggtaat
    48541 cattaataaa aggataaaaa aatgaaactg acaacacatc atctacggac aggggccgca
    48601 ttattgctgg ccggaattct gctggcaggt tgcgaccaga gtagcagcga tgcaaaacac
    48661 attaaagttg gcgttataaa tggcgcagaa caagatgtcg cggaagtcgc taaaaaggtg
    48721 gcaaaagaga aatatggcct cgatgttgaa ctggtaggat ttagcggttc gttgctaccg
    48781 aacgatgcga caaatcatgg cgaacttgat gcgaatgtct tccagcatcg accctttctt
    48841 gaacaagata atcaggcgca tggttataaa ctggtagcgg tgggaaatac gtttgtcttc
    48901 cctatggcgg gttattccaa aaaaatcaaa accgtggcgc aaataaaaga aggcgcgaca
    48961 gtggcgatcc ccaatgaccc gaccaacctt ggtcgcgcac ttttactgtt gcaaaaagag
    49021 aagctaatta ctctgaaaga gggaaaaggg ttattaccta ccgcgctgga tataaccgat
    49081 aatcctcgtc atttgcagat tatggaactc gagggggcgc agctcccgcg cgtactggat
    49141 gatcccaaag ttgatgtagc gattatcagc accacttaca ttcagcagac agggctttct
    49201 ccggtgcacg acagcgtatt tattgaagat aagaattcgc cgtatgtgaa tattttggtg
    49261 gcacgggaag ataataagaa tgcagaaaac gtgaaggaat ttctgcaatc ttatcaatca
    49321 cccgaagtcg ctaaagcggc agaaaccatt tttaacggtg gcgcggtgcc tggctggtaa
    49381 ttctcactca tgccgcgccg gacgatttat ttttctggcg cggcgacggg aatccattga
    49441 aatcaaaatt accgatgaca gaattagcag cgttcccagc cagtccggta atgtgaacgt
    49501 gatccccagc aacagcaaag agagtagcgc gctacttaat ggttctgcac agctcaaaat
    49561 gctggctttt ggaccgccaa ttaattgtgc tcctttcagg tacagactaa atgtcaggga
    49621 cgtaccaatg acgatcaaat aaaaaaacgc cagaatcaaa ctgccgttaa cgacaaagtt
    49681 tgttccttgt ctggcataaa aaggcaacag aatcagaccg ccaatcagca tactccagcc
    49741 gacgactggt aacgtgccat agcgggcaat tagtgttgag ggataggtgg tatagaatgc
    49801 agcagcaaat gccgaggcaa tgccccagaa caacgcggca ggagagatcg ataatgacgt
    49861 cggattaccg tgtgtcacca ataaaaaagt cccgaccagc gatgtcaaaa tagcgcagaa
    49921 aaccagaatg cccgggcgcg atttacgcac cagtgagaac caggcgacga taatcgtcgg
    49981 tgagaggaat tgcagcaccg ttgccgtggc tgcgttcgat ttttcgatgg ttagcaaaaa
    50041 agtgagctgt acagttagcg cgccaaccac ggaaaaaatc agcaggctaa tggcatcttt
    50101 atgattgtta ataatagaaa agattttatc gccatgaaca aatgacagcg tcagtagaat
    50161 caaaccggcg aatatcaaac gcgtcatagt caaaaactgc gacgacatct ggctttgctc
    50221 catgatgtat tgcgcacaga cccctgaact tccccacaac acggcggcaa tcagaacgtt
    50281 cagcatcccc tttctggtgg aacccatttt tccctctgca tactgttttg tcgtttatgt
    50341 atgcaggcat gatagcaaaa atggcgagga tggctctgtt tgcgccacgc cgatttttct
    50401 tagcccagac tggtgagcta aatatcttta ataaacagca ggcaaatcat cgcaataccc
    50461 aacatcccta tcgccagcca gaacaacgca tggtagctcc agatttcaac cataattccg
    50521 tcaacagagc cggcgataat ccagccgacg cgtgaagtat ttgcatataa cgttgtcgca
    50581 gagcctattt tttcaggcat caagtcctga aaataaagca tgccgatacc acagagaata
    50641 ccaaggaaga tggcattaag aatttgcaat tccagctcaa ccgccggagt cgtcgccatg
    50701 agtacgctgg cgtaaaaaca cattccactc acgatagcaa tgagcattaa tagtcgcttg
    50761 ccaatacgtt tcatgtaata gcctgcgatt aacatcatcg gaatttccag accggcagcg
    50821 atacaaatca tttctccagc cagtttatcg gttagatgca gttcatcaat aataaatagc
    50881 ggcatattta tcatgtagag attattcgcc gcccacatca ttgaacagac cacaaaaagt
    50941 agccgcgtat cccgcttcct gtgggtggag ggtaaaattt ctacgggttg ggtaacgaca
    51001 ggaatatttc tttgtattga tggcaaaaac aaccagacta tcagtccgca aacaacaaat
    51061 gcgatggcag cggtgagata catcacttta aaactaaatc ccattgccag ttcataagcg
    51121 agcggtggcc cgataaccca ggcaagcgag atctgagcac gtaaaaatgt actgaacatg
    51181 accgtctcac ggcctgttct gtcggcgtgt tcacgggcga gggcgaacat ttgcgggttt
    51241 gcggtggaag caaaactact cagaagtacg cccgttgaga ggagaatgaa gtagttgcga
    51301 ttccacgcaa aaagcgtgca ggccagcact ccaaataagc aacatagcag aatcagtaat
    51361 ttacggtcgc cttgtttatc ggagtgcctt gccagaaatt gactgaccag aattcccata
    51421 atagcgctac cggtgaagaa aaaatctacc attataggac gggctttcag ttcatctccg
    51481 aggaatatac ttagggtagg agtctgaaga gcgcccgcaa tacccgtcag aaaggcgaca
    51541 agtaaaaatg ccgcggcagt gagatcaagt atttttgatg gagtggtagc cgttttttgc
    51601 attcttctga atcaccgggc gacaaaatat aactgagtta caaataagta ccagtcgaat
    51661 gagtattgta ttaagaattt ttgagtatcg ctgatacaac cagaaagcaa tgctagcgca
    51721 atattatagg gccagcaatt gagattatta gcaaaagcat gttaagaatt aattaccgaa
    51781 tttatatatt aa
//
LOCUS       DAZKGS010000015        48832 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKGS010000015 DAZKGS010000000
VERSION     DAZKGS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            326..1543
                     /gene="alaA"
                     /locus_tag="ACHOQY_000918"
     CDS             326..1543
                     /gene="alaA"
                     /locus_tag="ACHOQY_000918"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9677064.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1627..2226
                     /gene="yfbR"
                     /locus_tag="ACHOQY_000919"
     CDS             1627..2226
                     /gene="yfbR"
                     /locus_tag="ACHOQY_000919"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9677065.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2285..4117)
                     /locus_tag="ACHOQY_000920"
     CDS             complement(2285..4117)
                     /locus_tag="ACHOQY_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9677066.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4204..4854)
                     /gene="hxpA"
                     /locus_tag="ACHOQY_000921"
     CDS             complement(4204..4854)
                     /gene="hxpA"
                     /locus_tag="ACHOQY_000921"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9677067.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4865..5359)
                     /locus_tag="ACHOQY_000922"
     CDS             complement(4865..5359)
                     /locus_tag="ACHOQY_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9677068.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5442..5897)
                     /gene="yfbV"
                     /locus_tag="ACHOQY_000923"
     CDS             complement(5442..5897)
                     /gene="yfbV"
                     /locus_tag="ACHOQY_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9677069.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6235..7437
                     /gene="ackA"
                     /locus_tag="ACHOQY_000924"
     CDS             6235..7437
                     /gene="ackA"
                     /locus_tag="ACHOQY_000924"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9677070.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7512..9656
                     /gene="pta"
                     /locus_tag="ACHOQY_000925"
     CDS             7512..9656
                     /gene="pta"
                     /locus_tag="ACHOQY_000925"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9677071.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9846..11366
                     /gene="yfcC"
                     /locus_tag="ACHOQY_000926"
     CDS             9846..11366
                     /gene="yfcC"
                     /locus_tag="ACHOQY_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9677072.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11399..11941)
                     /gene="yfcD"
                     /locus_tag="ACHOQY_000927"
     CDS             complement(11399..11941)
                     /gene="yfcD"
                     /locus_tag="ACHOQY_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9677073.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11999..12550)
                     /gene="yfcE"
                     /locus_tag="ACHOQY_000928"
     CDS             complement(11999..12550)
                     /gene="yfcE"
                     /locus_tag="ACHOQY_000928"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9677074.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12606..13250)
                     /gene="yfcF"
                     /locus_tag="ACHOQY_000929"
     CDS             complement(12606..13250)
                     /gene="yfcF"
                     /locus_tag="ACHOQY_000929"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9677075.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13386..14033
                     /gene="yfcG"
                     /locus_tag="ACHOQY_000930"
     CDS             13386..14033
                     /gene="yfcG"
                     /locus_tag="ACHOQY_000930"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9677076.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14090..14452
                     /gene="folX"
                     /locus_tag="ACHOQY_000931"
     CDS             14090..14452
                     /gene="folX"
                     /locus_tag="ACHOQY_000931"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9677077.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14473..15366
                     /locus_tag="ACHOQY_000932"
     CDS             14473..15366
                     /locus_tag="ACHOQY_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9677078.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15414..16304)
                     /gene="rpnB"
                     /locus_tag="ACHOQY_000933"
     CDS             complement(15414..16304)
                     /gene="rpnB"
                     /locus_tag="ACHOQY_000933"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP9677079.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16501..17274)
                     /gene="hisP"
                     /locus_tag="ACHOQY_000934"
     CDS             complement(16501..17274)
                     /gene="hisP"
                     /locus_tag="ACHOQY_000934"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9677080.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17282..17998)
                     /locus_tag="ACHOQY_000935"
     CDS             complement(17282..17998)
                     /locus_tag="ACHOQY_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9677081.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17995..18681)
                     /gene="hisQ"
                     /locus_tag="ACHOQY_000936"
     CDS             complement(17995..18681)
                     /gene="hisQ"
                     /locus_tag="ACHOQY_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9677082.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18771..19553)
                     /gene="hisJ"
                     /locus_tag="ACHOQY_000937"
     CDS             complement(18771..19553)
                     /gene="hisJ"
                     /locus_tag="ACHOQY_000937"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9677083.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19774..20556)
                     /gene="argT"
                     /locus_tag="ACHOQY_000938"
     CDS             complement(19774..20556)
                     /gene="argT"
                     /locus_tag="ACHOQY_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9677084.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20822..21391)
                     /gene="ubiX"
                     /locus_tag="ACHOQY_000939"
     CDS             complement(20822..21391)
                     /gene="ubiX"
                     /locus_tag="ACHOQY_000939"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9677085.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21486..23003)
                     /gene="purF"
                     /locus_tag="ACHOQY_000940"
     CDS             complement(21486..23003)
                     /gene="purF"
                     /locus_tag="ACHOQY_000940"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9677086.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23040..23528)
                     /gene="cvpA"
                     /locus_tag="ACHOQY_000941"
     CDS             complement(23040..23528)
                     /gene="cvpA"
                     /locus_tag="ACHOQY_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9677087.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGI
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23787..24449)
                     /gene="dedD"
                     /locus_tag="ACHOQY_000942"
     CDS             complement(23787..24449)
                     /gene="dedD"
                     /locus_tag="ACHOQY_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9677088.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24439..25707)
                     /gene="folC"
                     /locus_tag="ACHOQY_000943"
     CDS             complement(24439..25707)
                     /gene="folC"
                     /locus_tag="ACHOQY_000943"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9677089.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25777..26691)
                     /gene="accD"
                     /locus_tag="ACHOQY_000944"
     CDS             complement(25777..26691)
                     /gene="accD"
                     /locus_tag="ACHOQY_000944"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9677090.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26847..27506)
                     /locus_tag="ACHOQY_000945"
     CDS             complement(26847..27506)
                     /locus_tag="ACHOQY_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9677091.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27589..28401)
                     /gene="truA"
                     /locus_tag="ACHOQY_000946"
     CDS             complement(27589..28401)
                     /gene="truA"
                     /locus_tag="ACHOQY_000946"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9677092.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28401..29414)
                     /locus_tag="ACHOQY_000947"
     CDS             complement(28401..29414)
                     /locus_tag="ACHOQY_000947"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9677093.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29480..30616)
                     /gene="pdxB"
                     /locus_tag="ACHOQY_000948"
     CDS             complement(29480..30616)
                     /gene="pdxB"
                     /locus_tag="ACHOQY_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9677094.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30715..31710
                     /gene="flk"
                     /locus_tag="ACHOQY_000949"
     CDS             30715..31710
                     /gene="flk"
                     /locus_tag="ACHOQY_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9677095.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31707..32885)
                     /locus_tag="ACHOQY_000950"
     CDS             complement(31707..32885)
                     /locus_tag="ACHOQY_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677096.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33169..34389)
                     /gene="fabB"
                     /locus_tag="ACHOQY_000951"
     CDS             complement(33169..34389)
                     /gene="fabB"
                     /locus_tag="ACHOQY_000951"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9677097.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34548..36554
                     /gene="mnmC"
                     /locus_tag="ACHOQY_000952"
     CDS             34548..36554
                     /gene="mnmC"
                     /locus_tag="ACHOQY_000952"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9677098.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36675..36953)
                     /locus_tag="ACHOQY_000953"
     CDS             complement(36675..36953)
                     /locus_tag="ACHOQY_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9677099.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36987..37535)
                     /gene="epmC"
                     /locus_tag="ACHOQY_000954"
     CDS             complement(36987..37535)
                     /gene="epmC"
                     /locus_tag="ACHOQY_000954"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9677100.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37535..38344)
                     /locus_tag="ACHOQY_000955"
     CDS             complement(37535..38344)
                     /locus_tag="ACHOQY_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9677101.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38344..39168)
                     /gene="mepA"
                     /locus_tag="ACHOQY_000956"
     CDS             complement(38344..39168)
                     /gene="mepA"
                     /locus_tag="ACHOQY_000956"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9677102.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39172..40257)
                     /gene="aroC"
                     /locus_tag="ACHOQY_000957"
     CDS             complement(39172..40257)
                     /gene="aroC"
                     /locus_tag="ACHOQY_000957"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9677103.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40292..41224)
                     /gene="prmB"
                     /locus_tag="ACHOQY_000958"
     CDS             complement(40292..41224)
                     /gene="prmB"
                     /locus_tag="ACHOQY_000958"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9677104.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41390..41941
                     /gene="smrB"
                     /locus_tag="ACHOQY_000959"
     CDS             41390..41941
                     /gene="smrB"
                     /locus_tag="ACHOQY_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9677105.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42014..42865)
                     /locus_tag="ACHOQY_000960"
     CDS             complement(42014..42865)
                     /locus_tag="ACHOQY_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9677106.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVMPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42867..43406)
                     /locus_tag="ACHOQY_000961"
     CDS             complement(42867..43406)
                     /locus_tag="ACHOQY_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677107.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43403..43891)
                     /locus_tag="ACHOQY_000962"
     CDS             complement(43403..43891)
                     /locus_tag="ACHOQY_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677108.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43888..44397)
                     /locus_tag="ACHOQY_000963"
     CDS             complement(43888..44397)
                     /locus_tag="ACHOQY_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677109.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44413..45165)
                     /locus_tag="ACHOQY_000964"
     CDS             complement(44413..45165)
                     /locus_tag="ACHOQY_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9677110.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45185..47830)
                     /gene="yfcU"
                     /locus_tag="ACHOQY_000965"
     CDS             complement(45185..47830)
                     /gene="yfcU"
                     /locus_tag="ACHOQY_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFP9677111.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGIGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47912..48475)
                     /locus_tag="ACHOQY_000966"
     CDS             complement(47912..48475)
                     /locus_tag="ACHOQY_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677112.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11978 a        13192 c        12178 g        11484 t
ORIGIN      
        1 ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg aattcttaaa
       61 tgaggcattt tcacacaatt atcttacaga taaaaaaacc agacttacaa ttaagaatca
      121 gaacaattca ctatataaca ttgcatgtaa agcatataca cctcattatt ttgtcattat
      181 taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc tgcagacctt
      241 caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca gggagtggcg
      301 ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca aattagagaa
      361 tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg aagaagaagg
      421 taacaaggta ctgaaactga acatcggcaa cccagccccg ttcggttttg acgcgccaga
      481 tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt gcgattccaa
      541 aggtctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg gcatgcgtga
      601 tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat
      661 gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt acccactatg
      721 gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg atgaatcctc
      781 tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta cgcgtgggat
      841 cgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc ttttaatgga
      901 gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa tttatgacaa
      961 aattctttac gacgacgctg agcatcactc aattgcgccg ctggcacctg acctgctgac
     1021 cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat
     1081 ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg aaatgctggc
     1141 ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg cgctgggtgg
     1201 ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc agcgtaaccg
     1261 cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc gtggtgcgct
     1321 gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt cacgacgatc agaaaatggt
     1381 gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat tcaactggcc
     1441 gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt
     1501 gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat ttcactgccg
     1561 gagattgcat ccggcagcgt tatcccgcca caatgacctg atgatgtcat catacgtaag
     1621 gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact cattaaccgc
     1681 tggccgctca tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt gcaggtagcg
     1741 atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa tgtcaacgcc
     1801 gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac cggcgatctc
     1861 cctactccgg tgaaatactt caattcgcaa atcgctcagg agtacaaggc tattgaaaaa
     1921 atcgctcagc aaaaactggt cgatatggtt ccggaagagc tgcgggatat ctttgcgccg
     1981 ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca
     2041 ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga attcttgctg
     2101 gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat ggactacttc
     2161 atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca ggattcaccg
     2221 ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc gccggatgcg acatcaggct
     2281 cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc acgataatgg
     2341 tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga cctaaaacca
     2401 gtgtgttaac aggtgaagaa accggtgtca taaaggcggc ggatgctgcc atcgccacga
     2461 ccatcgcgaa tggataaggc gacaccccca tcgttttggc agcagccagc gcaatcggag
     2521 ccatcaacac cgccgtcgcg gtattagaga taaatagccc aataaccgcc gacaagacaa
     2581 acaaacaccc cagcatcata tgtggcccgt aaccaccgcc aatgtccatc agccctttca
     2641 ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc atcccaacga
     2701 tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct atacagcgga
     2761 atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt tcatctgtca
     2821 gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg
     2881 gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg
     2941 ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc agcagcagag
     3001 gctcatccgc cagcgaacct tccagcgcca cgccattgcg ctttagcccc accacattca
     3061 gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt tctgactcag
     3121 gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa tactcgccgc
     3181 gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg acatcagccg
     3241 cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca ttaacgttca
     3301 cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg ccataacgct
     3361 cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg cgaatagcca
     3421 gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag gttcgacgcg
     3481 tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc atcactaaca
     3541 tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta aagaaactga
     3601 agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc ggcgtcgcca
     3661 ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc agacgtgacg
     3721 gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata gcgacaacgc
     3781 cggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa accaacattt
     3841 tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg gcaacaccgg
     3901 tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg ttaggatcag
     3961 aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac gcgacaataa
     4021 caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg atggcaacgg
     4081 ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt taattgaggg
     4141 ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc atgatcaggt
     4201 gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt gctccagact
     4261 gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt taaccgcaat
     4321 gacatggcaa cccgccgcca ggccagaaag cacgccagcg ggagcatctt ccaccaccac
     4381 acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat caggttctgg
     4441 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
     4501 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
     4561 tgctttattc aaatgattga gtaaggcgat tgcccccgga agcgcggtaa taccttcggt
     4621 ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt
     4681 gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga aagccagcac
     4741 ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg
     4801 cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc
     4861 ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc
     4921 aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa
     4981 aaccgtgagt tccggcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga
     5041 cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt
     5101 catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca tgatacatct
     5161 ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca
     5221 attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga cggtaacgtt
     5281 cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt
     5341 gcgcgttggt catttccatt gttgactcct gtatcactat actacggtga aaaaaaagaa
     5401 ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa
     5461 gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc
     5521 ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa
     5581 aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat
     5641 ccctgcatgg gtaaacttaa ggcgaacagc gcagtggcaa ctgccggccc aagctgaccg
     5701 cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca
     5761 taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg tttttccagc
     5821 ggccacgtct ttgagtaatg ctgtccccgg cgaaacaagc taaaaaaatt aacagaacga
     5881 ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca
     5941 aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt
     6001 atcctttagc agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata
     6061 cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc
     6121 atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta
     6181 cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg
     6241 agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat
     6301 gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct gcccgaagca
     6361 cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct
     6421 cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct
     6481 gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc
     6541 gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg
     6601 cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa
     6661 gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac
     6721 ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac
     6781 ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa
     6841 gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac
     6901 ggtaaatgcg ttgacacctc tatgggcctg accccgctgg aaggtctggt catgggtacc
     6961 cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc
     7021 gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg
     7081 accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca
     7141 atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat
     7201 ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc gatggttcgt
     7261 gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg
     7321 gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt
     7381 atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt
     7441 cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa
     7501 gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga
     7561 ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt
     7621 tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg
     7681 tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag
     7741 gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg
     7801 ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc
     7861 agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg
     7921 ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca
     7981 acagcttcgg cggtgccaaa aacaccaaca tcaccggcgt tatcgtcaac aaactgaacg
     8041 caccggttga tgaacagggt cgtactcgcc cagatctgtc cgagattttc gacgactctt
     8101 ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc ccgctgccgg
     8161 ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc
     8221 gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg
     8281 tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc
     8341 tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg
     8401 gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta
     8461 aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct
     8521 ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgagc
     8581 gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc
     8641 tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc tgcgttccgt tatcagctga
     8701 ctgaacttgc gcgcaaagcg ggcaaacgta tcgtactgcc ggaaggtgac gaaccgcgta
     8761 ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta
     8821 atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactgggt gcagggattg
     8881 aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gaactgcgta
     8941 agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg
     9001 gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta
     9061 ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg
     9121 tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga
     9181 tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg
     9241 ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg
     9301 gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc
     9361 ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga
     9421 aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc ttcccggatc
     9481 tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg
     9541 ggccgatgct gcagggtatg cgcaagccgg ttaacgacct gtcccgtggc gcactggttg
     9601 acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaatctc
     9661 gtcatcatcc gcagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt
     9721 tttttattct cttaatctgc attaatcctt tctgattatc ttgcttaact gcgctgcatc
     9781 aatgaattgc gccatttcac tttgcatact taccactttg ttttgtgcaa gggaatattt
     9841 gcgctatgtc cgcaaccact gaatccaaac caacaagaag atgggcaatg cccgatacgt
     9901 tggtgattat cttttttgtt gctattttaa ccagccttgc cacctgggta gttccggtgg
     9961 ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag
    10021 tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc
    10081 gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag
    10141 gattaacctc aggatcgaaa tacgggacag ctgttggcat catcatgttt atgctggtga
    10201 ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc
    10261 ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt
    10321 cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg
    10381 caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg acctatattg
    10441 ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtc gttgctcagg
    10501 ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg
    10561 ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc
    10621 ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggct gatgttgaac
    10681 aacgtccgtt tacctttggt gactggctgg tattgattgt cctgaccgcc gtaatggtct
    10741 gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct
    10801 tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg
    10861 ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc cctgccctgc
    10921 tggtgggttt cgccaaaggg attttgctgc tggtcggtaa tggtgaagca ggtgatgcca
    10981 gcgtgttaaa taccatcctc aacagcattg ccaatgccat tagcggtctg gacaacgctg
    11041 tcgcggcctg gtttatgttg ctcttccagg cggtatttaa tttctttgtg acatccggtt
    11101 ctggtcaggc ggcgttaacc atgccgttac tggcaccgct tggcgatctg gtcggtgtta
    11161 accgtcaggt taccgtgctg gcattccagt ttggtgatgg cttcagccac atcatttacc
    11221 caacctcagc ttcgttaatg gcgacgctcg gtgtttgccg ggtggacttc cgtaactggc
    11281 tgaaggtggg cgcgacactg cttggactgc tgtttattat gtccagcgtc gtggtgatcg
    11341 gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc
    11401 attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca
    11461 gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca
    11521 gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcagc
    11581 tgaacaatgc gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcag
    11641 caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca
    11701 gttgctcatc ggcctggacc acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt
    11761 cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt
    11821 aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga
    11881 cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca
    11941 ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt
    12001 acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta
    12061 cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata ctcaccgagc
    12121 cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatggccgt
    12181 acaccagcac atcgttctgg tttaaagcag gcagattttc cgggccaaaa agatgaccat
    12241 gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga
    12301 aatgcagcag catttgatcc acttcgctgt cgcagttgcc gcgcacagcg ataaccttat
    12361 gtgccacttc attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat
    12421 tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttggg
    12481 caaacaactc cagaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca
    12541 tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt
    12601 tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc
    12661 cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg
    12721 cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc
    12781 tgacccagta ctaacaaatg ttctgccatc gcaaacagtt tctctgcact ggcttttccc
    12841 tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg
    12901 cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg cgcacgattt
    12961 tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac
    13021 tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg
    13081 cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc gaggtcgatg
    13141 gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa
    13201 ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg
    13261 gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat
    13321 actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag
    13381 ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc
    13441 tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt
    13501 ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt
    13561 ctcctgccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt ttgttgtatc
    13621 tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac
    13681 agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta
    13741 atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc
    13801 gtctttacca tgtactgaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact
    13861 acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg
    13921 acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca
    13981 ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac
    14041 aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc
    14101 tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa ttaaggaaga
    14161 agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa
    14221 agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat
    14281 tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc aggatgtgct
    14341 cgatatcgca cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca
    14401 cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt aaccgccata
    14461 tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg
    14521 attccgcgtt tgctggagct gggccatcaa atcacggtag tgacgcgtaa cccacagaaa
    14581 gccagttccg ttctcggccc tcgggtgacg ctatggcaag ggctagccga tcaaagcaac
    14641 ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg
    14701 actcacgagc aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc
    14761 gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc ggcaacgggc
    14821 tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt
    14881 actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg
    14941 cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa
    15001 atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg
    15061 gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga taacgacctg
    15121 cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg
    15181 ctcggtcaag cgctgcatcg cccggccatt ttgcgcgtcc ccgcaaccgc cattcggctg
    15241 ttaatgggcg aatcttcaat actggtatta ggtggacaac gcgcgctgcc taaacggctg
    15301 gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt
    15361 cgctgatgtg gtttacagca aacatccgcc agttaactcc cggtgttaca ggattagtgg
    15421 ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct
    15481 aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt
    15541 gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg
    15601 cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg
    15661 taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca
    15721 atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac
    15781 agcgccattt tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac
    15841 ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag
    15901 cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat
    15961 ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcatc
    16021 atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac ataaatataa
    16081 cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg ccgcaggtcg
    16141 tcatcaataa aactgtttgg ttccagttta agcgtcgtta aatcacacag tttgcgcagc
    16201 ggcgcgggaa gatgaatatc aataaaatcc cgcgcggtgt ctggatggcg taaaaaagat
    16261 ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt ccgtcacccc
    16321 tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca ggcagttttt
    16381 atctttggac agcgaattca gaagtatttt tagcggactg caacttttac atttcagcgg
    16441 aagctgactc acaaattccc catgaaaata ctccggcagc gttagccacc ggagaggaaa
    16501 ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt tgccaaataa
    16561 ctgctccggc gcgccctctt cttctatttt tccctgatgg aggaaaatga cgtgagtaga
    16621 aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc cctcttctgc
    16681 cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg ccgaggtagg
    16741 ttcatcaaac agtaaaactt ccggttccat cgccagcgcc cgcgcgatag aaacacgctg
    16801 ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt ctatcccgac
    16861 tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc ccaacacctg
    16921 aatcggcgct tccatgacgt tttccagcac cgtcatatgg ctccagagat tgaagtgctg
    16981 gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg cgactttgag
    17041 ttgaccatct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga tcgacccttc
    17101 actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg atcccgacga
    17161 tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt tcagcacttc
    17221 atgttcgccg tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg acatcgtatt
    17281 ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct ctgcgaaaga
    17341 ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg aaggcggtaa
    17401 aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcagatcc ggcacagtgg
    17461 ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg tatgccggta
    17521 acgcaatacg cagcgcagaa ggcaaaataa tgcaacgata cattttaaaa gtcgagaagc
    17581 cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc ccagcaaaaa
    17641 tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag ttcaggccac
    17701 tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg tacatgccgg
    17761 aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag gtaaataacc
    17821 agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga aacagcgcca
    17881 ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc acaccagtaa
    17941 agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt tcgatcacag
    18001 gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca caccattgga
    18061 aacagtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga acggttccca
    18121 ggtacttttg cctgccagtt gggtggcttt gaccacatct tccaggccga gtaacgaaac
    18181 cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca gggcgtaacg
    18241 catcatcgcc ggaaacatga tccgccgaaa cacttgccca cgagtaaaac cgaacgccgt
    18301 cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg tttcggtaaa
    18361 ataagcaccg tagataaaac cgagagtaat aataccagcg accatcggat cgatatcaat
    18421 ctgcccgacg cccatcgcct ccgtcaccgt gtttagtgca atctgcaaac cgtagaaaat
    18481 cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc cttcgaaaat
    18541 cagccccgaa agccgatttt gcgagagctt accgccagcg ccaattaaac cgatgattac
    18601 agcgagcact acagaactga tagccagctc cagcgtgacg agcgcaccct gtaaaataac
    18661 acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt accgcctctt
    18721 gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga ttaaccgcca
    18781 taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc agcgcgcatt
    18841 tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg caggcccatg
    18901 ccggtcccta cgccaaacag tttttcatct ttaacagacg ggccaccgaa tttgtaatct
    18961 ttaccgacag gttgtttgag gaaaccttcg ctggcagcga cctcatcctg gaacgcggca
    19021 tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata cgagacgatt
    19081 tcaatgcctt ttggtgccca atgttcatta ccgaacgtct cctgggtggt gccctgcaat
    19141 acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcgga atttttcgcc
    19201 accaccaaac gagaatcggc agcgtacagt ttgtcggtga aggctatttc ttgctgacgt
    19261 ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt taaggacggg
    19321 attaacgcat ccagcggatt ttcgacaaac gtacattgcg tattgatgcg tttgcataat
    19381 tcctttgcca gatcgatatc gaagccaacc agttcgcctt gtgaattttt tgattcaaat
    19441 ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaatcgcagc aaacgccgca
    19501 gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt cataatgtcc
    19561 tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat cgtgccataa
    19621 aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc attaaaaatg
    19681 cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc accatgatga
    19741 tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat taaagtcgaa
    19801 atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa gcgccttatt
    19861 gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc catcaccgaa
    19921 gtattttttg tcttttactg atgagccagc aaaggcgaaa tctttaccag caggttgctt
    19981 gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac gtcctgcagc
    20041 cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc ctttgctacg
    20101 ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa catgtttacc
    20161 tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca aacgagaatc
    20221 tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg taatggaaag
    20281 cgacgaaata atggcgtcga tttttttcgc tttcagtgag gggatcagcg cgtcaaagtc
    20341 actggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac cgagatcgat
    20401 atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacggcgcat aggtggtatc
    20461 ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg cggagagacc
    20521 gactaacaaa gacagagcga gaatcgactt cttcatacat aaccctcaag tgaaaatggc
    20581 ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg tcttatgcca
    20641 tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg cattattgtc
    20701 ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg aatggcagaa
    20761 acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg caacagcgga
    20821 gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca aactggtcaa
    20881 gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga tgataaaacg
    20941 ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt aaacgcagat
    21001 ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc tctttcagca
    21061 ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg ccggaaaggg
    21121 ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg gaagagatgc
    21181 tggcggaaat atcgcgcgca tcgtgcgtga catcagctaa tgcctgcact tcgcgcaggg
    21241 aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc agatgcgttt
    21301 cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc gcgccgctgg
    21361 caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga cttgcaggca
    21421 gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca ccctcagcga
    21481 aggcatcatc cttcgttatg catttcgaga ttttccactt cgttctgacg ttgcactgct
    21541 ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc aacatctttg
    21601 gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg attttcagcg
    21661 cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc accgatgatc
    21721 tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct cggcatatca
    21781 ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta cactttcttc
    21841 gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg gacgatggag
    21901 tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt cagtttacgg
    21961 cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc aacatagcgg
    22021 tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc cagcgcgata
    22081 tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc ccattcgcgg
    22141 gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac ggaaattttg
    22201 tcgatgaacg agtccgggcg agcaaagtat acatactcaa acaggcacgg attgctgacc
    22261 ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta aatcgcttcg
    22321 cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac gctttcggaa
    22381 gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac cagcggacga
    22441 atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc cacacaggca
    22501 tatgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt gtcggcttcc
    22561 agcgggtagt ggcggaagtt gtccagctcg ctagcgaaga tattaagcag aatttccgag
    22621 tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg caactcgtga
    22681 gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggagagtt aacgtaaaac
    22741 ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg accaatgccc
    22801 atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac cagcccgttc
    22861 gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc ggcatcctga
    22921 ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg cataacaccg
    22981 gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc tcagagcact
    23041 tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca tctgatgata
    23101 aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cgcttttcga caccccggta
    23161 aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa cgcaccgaaa
    23221 cagatgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa ctggcctatc
    23281 acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc gatggcaatt
    23341 ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta agtgtagtaa
    23401 tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc acgaacaaag
    23461 ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc gtaatcaatc
    23521 cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg cattctaaca
    23581 gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa tggcgctgaa
    23641 aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg cgtgtcggat
    23701 gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaggcctg ataagacgcg
    23761 ccagcgtcgc atcaggcaag actgtattaa ttcggcgtat agcccattac cacgccactt
    23821 aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt cgaggcatcc
    23881 ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga cgtataaacc
    23941 cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc atttttcagc
    24001 gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc cacgaccggc
    24061 ttcggttctg gcttcggcgc aggtggtgct tcaaccttcg gtttaggcgg ctccaccggt
    24121 ttcgacttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt attggctgca
    24181 atagtggtcg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc ttccgctgcg
    24241 ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc aggctcatcg
    24301 cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata atgttttttc
    24361 tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac gatcgtgccc
    24421 actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca cttccatgac
    24481 atgtgcgaca gtgtggaaag aaccacacac cagcacggtg tcttccgctt tagcgtccgc
    24541 cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac ccaaatgctc
    24601 aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat accagtcatc
    24661 aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta gcataccgat
    24721 aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga gatattccgc
    24781 cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca caatctggaa
    24841 acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac tgacttccag
    24901 cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga caagcggcaa
    24961 cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat cggtgacgga
    25021 atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgtg ccacatcagc
    25081 aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc ggaagatgcc
    25141 tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat gatccagcgc
    25201 aatactggtt actaccgcga catcggcatc gacaatattg gttgcgtcca gacgaccgcc
    25261 cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc acaacgccga
    25321 cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt caatctccgc
    25381 aaaagaggcg gtgtgggccg attccggcaa ttcctggccc tgcacacgta cgcgctcggt
    25441 ataacgcacc agatgcggtg aactgtagac gcccactttg taccctgccg ccatcagaat
    25501 cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg taaacacaaa
    25561 tggcgctggt ttcagaacgc caagacgcgc cgcgacctgg ctcacgcgct caaggccgag
    25621 atcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca gaggcgacgc
    25681 ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt gagaatagca
    25741 aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt cctgatccgg
    25801 taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg gcagattcat
    25861 caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga cgatcatgtc
    25921 gatcgcgcct ttctcgatca ggaattcact gcgctggaat cctggcggca gtttttcgcg
    25981 aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttcg gttcagcgat
    26041 gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg ggtcggtcag
    26101 cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag aggttttcgc
    26161 catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg cggagaagca
    26221 gatcagcggg cagttatctt ccagcgcctg ctcaacggca cgcacgaaac gtgcacccac
    26281 aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga caaccggcat
    26341 tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccagttt ctttctgcgc
    26401 agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagcacat ctttcggctc
    26461 aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca ggcgattacg
    26521 cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac gttccagctc
    26581 agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt caggaatgct
    26641 cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca ttagggacct
    26701 ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat gccatttttg
    26761 cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact ttggataaaa
    26821 aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc gcgtttgtga
    26881 cgaataaatt cgaaaacccc aggcaatatt gaaacaaaaa taatgccaac aatcatcagc
    26941 ttaaggttgc tctgaatgaa ggggagcgtg ccgaagaaat aacccgcgta ggtaaaaatc
    27001 agtacccaca acagtgcgcc gatcacgtta taagcggcga aatgacggta cgacatgtgg
    27061 cccattcccg caacaaacgg cgcgaacgtt ctgacgatcg gtacaaaacg ggcgagaata
    27121 atcgttttgc cgccatgtct ctcgtaaaac tgatgagtct tatcaagata actgcgacga
    27181 aagattttcg aatccgggtt actgaacagt ttgtcaccaa ataaccgtcc aatggtatag
    27241 ttcactgcat caccgacaat agcggcaatc aacataagca ccaccatcat atggacattg
    27301 agatcgttag tttccagcga cgccaatgca ccagcaacaa acagcagcga atcaccaggc
    27361 aggaatggcg ttaccaccag ccccgtttcg cagaacagaa tcaaaaacag aatggcataa
    27421 acccagacgc cgtactccgc gaccagctct gccagatgca cgtcaatgtg caggataaaa
    27481 tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc atctttcagg
    27541 ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt agtccgccag
    27601 aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat ccaccgcgac
    27661 cagatacagc ccttccgctt ttgccgttgc tgccgcccgc gttctgtcct ttgccgccag
    27721 caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca ggctgccgac
    27781 aatattcctg accatatgat gtacaaaggc attcgctttg atatctacca ccacataagg
    27841 accgtgacgc atgacattaa tatgcataac gttgcgccac ggggttcggg actggcactg
    27901 caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag cccgatgcat
    27961 ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta ccgccgggcg
    28021 cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa atcgggcatg
    28081 aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta aattcgcatt
    28141 tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat gcaccacttg
    28201 cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga tgggttcgtt
    28261 cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt cattctgccg
    28321 ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt tataaactgg
    28381 cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc ggcgattttt
    28441 actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa ctggacttgc
    28501 tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt acccgaagca
    28561 tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc gccctgagca
    28621 aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt gaccatctgg
    28681 gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag cccttcgtcc
    28741 tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct accttcgcta
    28801 tccggcaaca acggcagcat attgaacgcc agttgacgac cgaagaaatc ttcttcgtca
    28861 atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac cgcttttttg
    28921 ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc gccctgatcg
    28981 attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac ggcgatgaca
    29041 ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac cagcggtacg
    29101 tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga gttggtcgct
    29161 tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag ctgcgcctgc
    29221 gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa gcgcagttgt
    29281 tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa ctgacgttca
    29341 gccagcgttt caagcagggc ttcgcccaca gcgccagttg cgcccaggac ggcaatgttc
    29401 cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca atgttacagc
    29461 agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa cccagtttac
    29521 acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac cattcacggc
    29581 gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg actttacgca
    29641 gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc agcgtcggtt
    29701 gatcgagcgg gccatgcagc gtaatgcgac caaactctgg cgcgggcagt aatgtatcca
    29761 gcgcaacgtg ctgttcatgc ccaataaact tgctataggc ttcaaacact tgcgtagtac
    29821 cgcgtgcttt accttccagg gtataacctg cgatatgcgg cgtgccgata tccacttttg
    29881 tcagcaactc cacgttaagt tccggttcgc cttcccagac atccagcact acgcttaact
    29941 tctggccttc attcaggcag gtcagcaacg cagtattatc gacgactgcg ccacggcagg
    30001 cgttaatcag aatcgctccc ggcttcaggc tacggatcag tttttcatcc gccagatgca
    30061 gcgttttgta cggcccatct ttgaagagtg gcgtatggaa agtcagaata tccgcatgct
    30121 gaactaattc atccagcgag cggaaatcac cctcatcccc acggtcagcg cgaggcggat
    30181 cgcaaagcaa tgttttaatc cctaacgctt ccagtcgcgc ctgcaatcga cgtccaacgt
    30241 taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg cgttcggcaa
    30301 gcatcagtag tgaggagaaa acatattcca ccaccgcaat cgcattacag ccaggtgcag
    30361 cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg gtccccgcag
    30421 tggcagtgcc aacaaattta atgggttttc ctgccagcaa agattcattc actttcgtga
    30481 ccgaacgcac catcagcgca tccgcgtctg ccagttgagc aacggggatt ggacgcccgg
    30541 gaaccgcggt cacctcaccc aaacggctaa ataagtcgcg ggcataaggc atattttcat
    30601 caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg gacaagtgtg
    30661 ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata cgtaatgata
    30721 caacctattt ccggccctcc tcctgggcaa ccaccaggtc agggagataa cctgccgtct
    30781 ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag cttaatgacg
    30841 aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat caggcacgat
    30901 attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc tgagcataat
    30961 ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct tttagcgcaa
    31021 ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta tatccgtcat
    31081 aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac cattctcacc
    31141 ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc gaccgaccgt
    31201 cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact tgcggcggca
    31261 acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg ggtgaaagat
    31321 ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggttgca ggcgcgccag
    31381 acgttaagcc agcaaaatac gccgacgctg gaatcactac agatggcgct aaaacaacct
    31441 ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata tggcctttct
    31501 gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct ttatcaacgg
    31561 cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt tccaccgatg
    31621 atggacacgt tgcaaaatat ggcaacgcgt cccgcgctgt ggatactgtt agtcgcgatt
    31681 atcctgatgc tggtctggct ggttcgttag ccccgacgaa atgacagtat cgtgacaata
    31741 attcccagca ccgcagagat cgccccggca agaaataccg aagagtaacc aaacgtggtc
    31801 gccagcattc ccgccagcgg cccggagaca ccgagggcga tatcctgaaa cgcggcgtaa
    31861 ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc cacgcccagc
    31921 gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa tgcgacccac
    31981 gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag agagacaatc
    32041 gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg catcacgaca
    32101 aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt gctggcaaag
    32161 tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc cagccctaac
    32221 cctggtttcc agataaaccc gacaacgctc cacagcgatg gacgttctcc cgccagggcc
    32281 ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac cattgtggtg
    32341 atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg agcaccaaca
    32401 gcgagggcac cgtaaatcgc cattccattc catgacatca ctttgccaga gtgttttggc
    32461 cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc accaaaccca
    32521 agaatcaaac gcccgatgac caacagggca aatttgaacg gtgcggagac aggcaaaatc
    32581 gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg aagcgccgaa
    32641 cgttttgcac catattgatc ggccagtcgc ccggcatagc cacgcgtcag caccgtagcc
    32701 agaaactgaa tcccgacggc aatcccgacc atggtattgc catagcccag atcatgatga
    32761 acaaacagcg ggataaccgg caacggcagc cctacggtca tgtaggtgag aaaaaccgca
    32821 aaagcgatgc ggaagagcga aaaattggca gaagatcgtg tttcggtttg gcttacagca
    32881 gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc atctctctgg
    32941 tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga aggaaatcga
    33001 caaagcggga agtttgcctg gaacaggcgg cgattgtcaa tgatgtgaaa aagggaacca
    33061 tcaggttccc ttttgcgtta gtgccggagg ccgcattagc gcgtaacgtc ggatgcgacg
    33121 ctggcgcgtc ttatccgacc tactccgact actccggcct actgcgaatt aatctttcag
    33181 cttgcgcatt accagcgtgg cgttggtgcc gccgaagccg aagctgttag acataacggt
    33241 ggtcagttcg cgatcggtcg tttcggtcac gatgttcaga cctgcagcct gctcgtccag
    33301 ctcttcaatg ttgatgctcg gggcgataaa gccgtgttcc agcatcagca gagagtagat
    33361 agcttcctgt acgccagcag cgcccagaga gtgaccggtc atggctttgg ttgcagaaat
    33421 cgccgggctc ttatcgccga acacttcacg gatagctgcc agttctttca cgtcgccaac
    33481 cggagtcgaa gtaccatgag agttcaggta atcgattggg gtatcaacgc cgtgcatcgc
    33541 catcttcatg cagcgtactg cgccttcgcc agacggagca accatgtctg caccatcaga
    33601 ggttgcgccg tagccaacga tttcagcata gatgtgagca ccacgcgcca gcgcgtgttc
    33661 cagctcttca accactacca taccgccgcc gccagcgata acgaaaccgt cacggtgagc
    33721 gtcgtaagta cgggaggctt tttccggggt gtcgttgtat ttagtagaca gcgcacccat
    33781 cgcgtcgaat tcgcaggcca tttcccagca cagctcttcg ccgccgccag caaacacgat
    33841 gtcctgtttg cccagttgga tctgctctac tgcgttaccg atacagtgtg cggaagtcgc
    33901 acacgcggag ctgatggagt agttaacgcc atgaatttta aacggcgtgg cgaggcaggc
    33961 agaaacgccg gatgccatcg ctttggtgac cacatacggg ccaaccgctt tcaggccgcg
    34021 cgggccgcgc attgcgtcag cgccgaacac ctggaaacgc ggggaaccgc cgccagaacc
    34081 tgcaatcagg ccaacgcgcg ggttattctg gtaagcttcc ggagaaaggc ccgcatctgc
    34141 gattgcctgc tccatagaaa ggaatgcata aatggatgcg tcgctcataa agcgcacaac
    34201 tttgcggtca atgaggccag tggtatccag ttttacgttg ccccagacgt ggctacgcat
    34261 gccggaatcc ttcagctcct gagagaaagt gatccctgaa cgtccttcac gcagagatgc
    34321 caggacttcc tgctggttat taccgatgct ggaaacaatg cccaggccag taatcactgc
    34381 acgtttcatt caatacctct gtaagtcgca catagagtaa gtttcgaatg cacaatagcg
    34441 tacacttgta cgccgaacaa gtccgatcag ccatttaata gagaaatttg cgcagcctta
    34501 cacacatcgc taagatcgag ccaccgcctg taagacgagt aacttacgtg aaacactact
    34561 ccatacaacc tgccaacctc gaatttaatg ctgagggtac acctgtttcc cgagattttg
    34621 acgatgtcta tttttccaac gataacgggc tggaagagac gcgttatgtt tttctgggag
    34681 gcaaccaatt agaggtacgc tttcctgagc atccacatcc tctgtttgtg gtagcagaga
    34741 gcggcttcgg caccggatta aacttcctga cgctatggca ggcatttaat cagtttcgcg
    34801 aagcgcatcc gcaagcgcaa ttacaacgct tacatttcat tagttttgag aaatttcccc
    34861 tcacccgtgc ggatttagcc ttagcgcatc aacactggcc ggaactggct ccgtgggcag
    34921 aacaacttca ggcgcagtgg ccaatgccct tgcccggttg ccatcgttta ttgctcgatg
    34981 aaggccgcgt gacgctggat ttatggtttg gcgatattaa cgaactgacc agccaactgg
    35041 acgattcgct aaatcaaaaa gtagatgcct ggtttctgga cggctttgcg ccagcgaaaa
    35101 acccggatat gtggacgcaa aatctgttta acgccatggc aaggttggcg cgtccgggcg
    35161 gcacgctggc gacatttacg tctgccggtt ttgtccgccg cggtttgcag gacgccggat
    35221 tcacgatgca aaaacgtaag ggctttgggc gcaaacggga aatgctttgc ggggtgatgg
    35281 aacagacatt accgctcccc tgctccgcgc cgtggtttaa ccgcacgggc agcagcaaac
    35341 gggaagcggc gattatcggc ggtggtattg ccagcgcgtt gttgtcgctg gcgctattac
    35401 ggcgcggctg gcaggtaacg ctttattgcg cggatgaggc ccccgcactg ggtgcttccg
    35461 gcaatcgcca gggggcgctg tatccgttat taagcaaaca cgatgaggcg ctaaaccgct
    35521 ttttctctaa tgcgtttact tttgctcgtc ggttttacga ccaattaccc gttaaatttg
    35581 atcatgactg gtgcggcgtc acgcagttag gctgggatga gaaaagccag cataaaatcg
    35641 cacagatgtt gtcaatggat ttacccgcag aactggctgt agccgttgag gcaaatgcgg
    35701 ttgaacaaat tacgggcgtt gcgacaaatt gcagcggcat tacttatccg caaggtggtt
    35761 ggctgtgccc agcagaactg acccgtaatg tgctggaact ggcgcaacag cagggtttgc
    35821 agatttatta tcaatatcag ttacagaatt tatcccgtaa ggatgactgt tggttgttga
    35881 attttgcagg agatcagcaa gcaacacaca gcgtagtggt actggcgaac gggcatcaaa
    35941 tcagccgatt cagccaaacg tcgactctcc cggtgtattc ggttgccggg caggtcagcc
    36001 atattccgac aacgccggaa ctggcagagc tgaagcaggt gctgtgctat gacggttatc
    36061 tcacgccaca aaatccggcg aatcaacatc attgtattgg tgccagttat catcgcggca
    36121 gcgaagatac ggcgtacagt gaggacgatc agcagcagaa tcgccagcgg ttgattgatt
    36181 gtttcccgca ggcacagtgg gcaaaagagg ttgatgtcag tgataaagag gcgcgctgcg
    36241 gtgtgcgttg tgccacccgc gatcatctgc caatggtagg caatgttccc gattatgagg
    36301 caacactcgt ggaatatgcg tcgttggcgg agcagaaaga tgaggcggta agcgcgccgg
    36361 tttttgacga tctctttatg tttgcggctt taggttctcg cggtttgtgt tctgccccgc
    36421 tgtgtgccga gattctggcg gcgcagatga gcgacgaacc gattccgatg gatgccagta
    36481 cgctggcggc gttaaacccg aatcggttat gggtgcggaa attgttgaag ggtaaagcgg
    36541 ttaaggcggg gtaatctgct ctcgcagtgt ttgccggatg cggcgtgaac gccttatccg
    36601 acctacgtgt gacgtgtagg cctgataaga cgcgataagc gtcgcatcag gcatggtgct
    36661 ccagacgccg caacttactg ctgtgacgcc tgctgaaaca ggttttccca catatcggtc
    36721 accagcgcct ggtcacgcgg cgacaattca ccggcaccaa tggctttttc cagactctgg
    36781 ctaacggtcg tatgcaccgc ctgagcggag tggtcatcac cactttccag ttctgcgatg
    36841 gctaatgtca ggtggccacg caaataccca ctggcaaaca actcatcatc actggcatgg
    36901 tcaaccatac cgtcgattaa tgccagaatg cgtgattcaa actccgcgat catcttcttt
    36961 cctcattgta aaacgtggca ccggcttcag ttgagcgctt ccggccacgg gaactgttcc
    37021 gccgtaagtt ccggcgtgtg ataataattt tgtagtgctt taatgaagcg tgccgggcgt
    37081 tcaggaatgc cgtttgctag gtaatccatc acctgcgcat gtacccgccg ttgaaacacc
    37141 acacgatccg gttcgaagtc gccttccaga ttgtcgcaac tgacgttaaa cggatatccc
    37201 gccgccacac agaacaacca gtcgaacgcc tgcggcttca cttcaacatc ttcaaactgg
    37261 ctttgcgttt gggcatcgcg tccgtccggg caataccagt agccgaaatc aaccagctca
    37321 cggcgcgctt tcccagcaat acaccagtgc gaaatctcat gaattgcgct ggcataaaag
    37381 ccatgagcaa agacgattcg gttatacggt acttccgcat cagcaggaag atagatcggt
    37441 tcgtcgtcgc ctttaatcag acgggtatta aattcatcgg caaagcagct attaaaaatt
    37501 tcaatcaact gctcatagtg gtgtgtactg ttcattagtt catccccaac cagtggagga
    37561 tctcctgtcc gtggctatca aaaagtaatt tggcactcat caccgctgag acaataacaa
    37621 tcatcgggcg gatcagcttt tgtcctttgc tcaacaccag tcgtgaaccc atgcgcgcgc
    37681 cgaggaactg ccccaccagc atgacaaagc ccgtcgccca aatcactttg ccaccgagaa
    37741 taaacagcag taaaccgccg atgtttgacg ttgcgttgag taatttggcg tgcgccgtgg
    37801 ctttggcgag gttgaatccg cacagcgtaa caaaggccag tgcgtaaaac gatccggctg
    37861 ccggaccaaa gaatccgtca taaaaaccga cgcagccacc ggcaattaac gcgaagggta
    37921 aaccgtacat ccgacgctgg cgatcttctt cacccagctt tggcatcagc aagaaataga
    37981 ggccgataca aattaccaga atgggcaaaa tctgccgtaa gacatcagcc tgaacgtatt
    38041 gcaccagcag tgcgccgctc attgagccga caaaggtcat ggcgatattg agtttctgat
    38101 cgcttaaact aaccactttg cggcgaataa agtagatagt agcggaaata gagccgccgc
    38161 aggcttgcag tttattggtt gccagcgcat tagcgggaga catccccgct gccatcaatg
    38221 ccggaatggt gagtaaccca ccgccaccgg caatcgagtc gataaatccc gccagcatgg
    38281 cgacaaaaaa gagaactccc agcaacagcg gggaaaccat aaacaggcta ttaaacgttt
    38341 ccattagatc acgtgctcat ccagtagcgc ctggcaggaa ggcggcaacg gaggcggtgt
    38401 cttcttctca ggctttgttg ttcccggttt tggaggttca aaccagcttt gcagttctgc
    38461 cccgcaacca tcgcctggtg gcggtaaagg ttgatcttca cactccagac tatcggcagg
    38521 acaacgtaat cgtacatgca tatgcgcacg atgctggaac cagggtcgca ctttgcgcaa
    38581 ccagtcgcga tcggtgcccg catctaggca aagttgttgt ttaatcgccg gattaacaaa
    38641 aatgcgcgtg acgtttttgt cctgggcggc gagtttgatc aagctgaaaa tttctggctt
    38701 ccacagcgag gagacaacgt gtttaccgtc gcgggaaact aagtccagtg cttgcgggcg
    38761 caagagctgc gcggaggtcc agcgagtttt cggcagttgc agaaagatat cgacatccag
    38821 tccggtctgg tggctggcat gaccgccgtt gaaacgccca ccagcaggca tccccatatc
    38881 gccaatcagc accgtaccca tgcccagatt gctcacctgg ctactcagac gctggataaa
    38941 catcaccaga tccgggtgac cgaaatagcg acgctgatcg gtacgcatga cctgataatg
    39001 ttcggactgt atcggcagcg tgtcagcgcc gacaatacag ccattagaaa aactgcctat
    39061 cgattgtgcg ctacccggca caggttgggt tattttttgc cacggcgtcg ctgccaggct
    39121 agcgctactg gcaagcagag ccagcagcgc aatcgcggtt ttgttcattt tttaccagcg
    39181 tggaatatca gtcttcacat cggcattttg cgcccgctgc cgtaacaggt gatccattaa
    39241 aacgatcgcc agcatcgctt ctgcgatcgg cactgcacgg atcccgacac agggatcgtg
    39301 acggcctttg gtgatcatct caacttcttc gccaaagcgg ttaatagtgc gccccggcac
    39361 ggtaatgctg gaggtcggtt tcagcgccat atgggcaatg atttgctgcc cgctgctgat
    39421 accgccgaga atgccgcccg catggttgct ctggaaaccg tctttggtga tttcgtcgcg
    39481 gttctggctg ccgcgcagcg ccactacgtc aaaaccgtcg ccaatttcca cgcctttcac
    39541 cgcgttgatg ctcatcagcg catgggcgat gtcggcatcc aggcggtcaa agaccggctc
    39601 gccaagtccg gcggggacac cactggcaac aacggtgact ttcgcgccga tggagtcgcc
    39661 ctcttttttc agcgcgcgca tcagttcatc taacgcgtcg attttgtccg ggtccgggca
    39721 gaagaacgga ttttgttcaa ccagcgacca gtctttgatt tccagcggaa tgtcgcccat
    39781 ctgggtcaag cagccgcgga tttcaatacc aaatttctcg gcgagatatt ttttggcaat
    39841 cgcccctgcc gccacgcgca tggcggtttc gcgggcggaa gaacgtccac cgccgcgata
    39901 atcgcgcaga ccgtattttt gttcgtaggt gtaatcagca tggcctgggc ggaaaacgtc
    39961 tttaattgcg ctgtaatcct gagagcgctg gtcagtgttt tcgatcaaca agccaatgct
    40021 ggtgccagta gtaacgcctt caaaaacacc ggagagaatt ttgacctgat ccggctcgcg
    40081 gcgctgggtg gtatagcgcg atgtcccagg gcgacgacgg tcgaggtcat gttgcaggtc
    40141 cgcttccgtc agcggaatgc ctggcggaac accatcgacg atgcagccga gcgccagccc
    40201 atgcgattcg ccgaaggtgg ttacgcgaaa gagttgtcca attgtgtttc cagccatcac
    40261 ggctccgtta ttgttgtgtt tgcgtgttta cttaatcttt ataaatcgcg aaatattcac
    40321 gggccgccag cagctgctct ttggtgagca taaacacacc atcgccgccg ttatcaaact
    40381 ccagccaggt gaacggaaca tccggatatt gttccataag atgtaccatg ctgttgccga
    40441 cttcacaaat cagcacacca tcatcagcaa ggtaatctgc cgcgttaccg agaatgcgac
    40501 gcgtcagttt caggccgtcg gtgccagatg ccaggcccag ttccggctcg tgacggtatt
    40561 cgtttggcag gtcggacata tcttccgcgt cgacatacgg cgggttagtg acaatcagat
    40621 cgtattgcac tttcggcaaa tcacggaaca gatcggaacg aatcggaatg acgttgtgga
    40681 tcagaccgtg ttcttcgatg ttctgttcgg caaccgccag cgcgtcggga gagatatcta
    40741 ccgcgtcgac ttccgcttcc gggaaggcat aagcacaggc aatggcgatg cagccgctac
    40801 cggtacacat atctaaaata tgctgcggtt gcttgctgat gagtccggca aatttattgt
    40861 tgatcagttc accaatcggt gagcgcggca ccagcacgcg ttcatcgacg taaaattcat
    40921 gaccgcagaa ccaggctttg ttggtcaggt aagccaccgg aatgcgctcg ttgacgcggc
    40981 ggatcacgcg ttcaacaata cggtgttttt cgctggaggt cagacgcgcg gtgcgcatat
    41041 cttccgggat atccagcggc aggtagagcg atggtaacac cagctgtacg gcttcatccc
    41101 acgggttatc ggtgccgtga ccgtaccaga tatttgccgc gctgaagcgg cttaccgacc
    41161 agcgcaacat gtcctgaatg gtttgcagct catttactgc ttcatcaacg aaaattttat
    41221 ccacgtattc ctccagggca tgatcgcaat aatttcggcg gctagtttgc catgaagatg
    41281 acgataaatc agcattcacg cgcggtgagt gaggaaaaat acgtttaaaa cgatcgattg
    41341 tgctacgagt cgggtaaact gtaggaaaat tagaaataga gacagataaa tgaaaaagaa
    41401 aacaacactt agcgaggagg accaggctct gtttcgccag ttgatggcgg ggactcgcaa
    41461 gattaagcag gacacgattg tccaccgacc gcagcgtaaa aaaatcagcg aagtgccggt
    41521 gaaacgcttg atccaggagc aggctgatgc cagccattat ttctccgatg agtttcagcc
    41581 gttattaaat accgaaggtc cggtgaaata tgttcgcccg gatgtcagcc attttgaggc
    41641 gaagaaactg cgccgcggcg attattcacc ggaattgttt ttggatttac acggtctgac
    41701 gcagctgcag gccaagcagg aactgggcgc gttgattgcc gcctgtcgcc gcgaacatgt
    41761 gttttgtgcc tgcgtgatgc atgggcacgg gaagcatatt ttgaagcaac aaacaccgct
    41821 gtggctggca caacatccgc atgtgatggc atttcaccag gcaccgaaag agtacggcgg
    41881 tgatgcggct ttgttggtgt tgattgaagt ggaagagtgg ttgccgccgg agttgccctg
    41941 aggagttgag cgtgttgtgc caggagcgcg gaatggcagg ggcgaatgac cctgccaggt
    42001 gagaaaaggt gcgctaatac atattcagta tgtaagtgaa tcggcaggta gaatctatgg
    42061 gctgtcgaga tgtcgtgtcc acgcgtagct tcatatcatg cagttgcctg gacgtgatcg
    42121 gttgcggctc tggcatcacc gtcactttgg cattatcaca gccggaagtc gagaaggtca
    42181 tctgctgtgc gacaaaacgg cagtaggctc cacgctcgtc aaaattactg ccatcacaca
    42241 gtttgccgtt ttccatcagg aaagtgggca gtgtttgctc aataacacca cctgttgact
    42301 gcaacctgac gactttgtta tgtacaacat aattttcatt ccaggaaaca tcatacggtg
    42361 ccagggactc tgtcgggcag tcagagctaa cttttgttgg tcctataaaa attccggccc
    42421 cgaaacgctg atccatagta ctggcgcttt caacataaga cgacacaatc cagggacaac
    42481 cgtgtaaatc tccatcgtaa taaacgccaa gtactaattc attacatgac caggccgtta
    42541 ctggagagag agcatcatac tcttctaatc cggggcaggt agaatccggt aaggttgtat
    42601 tattgcgctc catcctgagt tcagggccgg gaggactccc ggtccaacta actaaaggca
    42661 aacttgctgc cgcagttgaa ttagattcat acgtgccata ttcaactgat tgaggggtca
    42721 ggactgaaag atgaaaacga tactttgcaa caacgctggt cgtccccgcg ggtacataga
    42781 gtgtggcgat ctttgtcgca gcctgtatcc cagaagcgca cagagccagc accaataaac
    42841 atataacatg acgtatttta tgcattttat atatactcaa acgtagctgt caggctggcg
    42901 gtgaaatcac cgatggttac atcacgaccg cttttcgcct ggatccaggc ccggaaatta
    42961 atatcattgt tgccgttggt aagcgcaaaa gtcgccccgg tggtgttgtt aatatccacc
    43021 cgcttgccgt cgagcgtttc catccctatc cccacgccct gcgcggtgga ggtgtcgtcg
    43081 actgccagaa aacccggctg accgctgtct tccgttccgg tgagggtgac tttgacgttg
    43141 tatcccgccg gccctttgca gtctttcagc ttaaacacaa ccggcagatg cgcggactgc
    43201 ccggcgacca tcaaatcccg gttgctgacg gtgggaaaat gaatttccgc cagattggca
    43261 ccatccacca ccagcgtgca ggatttactc agcaggttgc cgtaaaaatg caggttgtta
    43321 tcgactgccc gtgcccccgt gctggcggtc agcagcaggc ctgctaacag cgcataaccg
    43381 ggtgaataaa tgtgtgctgt tttcattggt aatccacccg taaggttgcg taagcagaaa
    43441 aattcgtgtc cgtcagcacc gcatcagccg ctttgaccgg taccgcctcc agctttggct
    43501 tctggctaaa atcggtggcg ttaatggaaa gcggctcgtt gagcttaaaa cgctgaccgt
    43561 catgctgaag ctcaattccg aagcccggta catccgtctc aatagcagag tcgttgtagg
    43621 aggtggcagt ccccgtccag gagagtgtca tattccactc gggatcgcgg acatccgagt
    43681 cgcagctcag ttcgtagtcc actttctgac gaccataatt gccatcaatg ctgtcgataa
    43741 taagatcgcg aaattgcact tcgatggttt gcccgccgct gatgctacag gcgggcggcg
    43801 acatcagcgt accgtggaat gtgatgtcat cttccgccgc cagcacagcc ccgcaaccag
    43861 cgcacagtaa aaggagcagg aattttttca ttggtaatcc accaccatgg tcatcgtggc
    43921 gttaaactcc gacggcgtca gttgcacccc gctctgtttt accggcaccg ctttcagctg
    43981 aggctgagtg ttgacattaa aaggcgtcca gttattttca ccaactgcaa acagggagtt
    44041 gtcggcggcg ttttctatcc gcacccccag gccggcgata tccgtgctga gaaccgtttc
    44101 tccgttgatc acggcggtat tgcctttgag ctgcatccgt aagtcatcgc tgactttatt
    44161 accgcagttg agggtgtatt tcgcatcctg ttggtaattg gtaccatcga tcctgttgat
    44221 tatcacattg ccaaatttca cagcgcttcc cttcacggag caaggcggcg gtccatcgac
    44281 caggattcgc agcgtcagat ccacctgttt gatatcactg ccagcaacag ccaaaccgct
    44341 gatgccccat ccggccagca ccagcaaccc tgcgcccgct acaaacttgt gactcataag
    44401 tccctcccgg catcagcctt tgcggctctg ctcttcatcc acctggcagc tgttgccggt
    44461 gcatatgaag tacagcggca tccgcgcgcc gtagtcgttg atgtaagtca gcaccggcac
    44521 gctgtccatc ttcgcattca gcggtgcgga ggttttcggc tccatcatca gcggtgtgaa
    44581 tcccgccggc gggttgccgc cttttttagc gctggcgttg ctgatgatca cgtagtacgg
    44641 cgtcgggtta ttgacggtga agtcctggcc gttacggatg agcgtcacct tatgctgcca
    44701 cggacttttc atgctggtgt tctccagcgc cttcggtcgc cagaacagtt tgatacgcgt
    44761 ctgcagcgca atctgcagtg tgttcggttt atttgatttc ggcggaattt cgcgcacgtt
    44821 gaaatagaac acgctctcgc ggtcggccgg cagtttgttg atatccggca gcccctgcac
    44881 cttcacctgg ccgttcatca tcgcatcgat acgctgcact ggcggaagca ccgcaagcgg
    44941 cgagctgatt ttgttgccct ttgcatcctc tatccagctc tgcgccagat aaggcagtgt
    45001 gggatcgttg ttgcgcagcg tgacgctgat ggacttgtcg ctttcgttaa acaccagccg
    45061 tgtcctgtcg ggcgtcaccg aagcgtggct ggagaaactg accactgtcg ccagcagcgc
    45121 cagtgccggg acaacgattt ttgcagtgtt agtcagatta aacatggatc attccttaat
    45181 cacgttactg gtttactgaa attgaagtcg gaggttccgt tggcgtcacc cgctgtgtct
    45241 gttcctgaat cgccggtttg accgccggcg aagtgatagg tgctacattg cctttatgct
    45301 ggcatggcag taacaagccg ttaaacagat cggcaggcag cgggtccggc aggttgatat
    45361 cgcaatgcgc aacaccactc cagaacacac tcatgtgttc gccaggtttc accccagcca
    45421 gataaacatt gccgtcatca tcgacaaggc ccactgtctg ctcgttatca ttttttactt
    45481 ctgcgccaaa cggaggatgg ctgccgtcgc tcaggcgcag caccgccatc gctttttgtc
    45541 cgctaatgac agcaaattta cggtagccaa tggcaccttc agttagcgtg gcttgcacca
    45601 ccgactgggt tgcttcagcg ttttcaggca atctgttgag gtcgatatac gcctgattgc
    45661 ggtaatagtt attgacgtca gaaacgacgg ctttaccaaa catattggta taaacagcag
    45721 ccccgttacc ttccaccgga acatcggcaa cgccatcggc atcaatcaac aatcgtgtcc
    45781 cgcccatatt ctgggtacgg tgaagtgcgc caccgtgggc agtaagcgtt gcgccgccct
    45841 gtaacgacaa gcccgcagag gtgtattgcc cttcatggta gttcgcactg aggtcaacct
    45901 gcgccagcga accatcatgg ctgtaatagc cgtcaacgct ggtgtgtttg tcactggtgc
    45961 cgacgttcaa ctgatagtga gtcgcgtcat cgacacggct gaaataaccg acctgactgc
    46021 tgtccgtccc actgccatag ttaccgttat agctaacggt actgttgtcg ccccacggca
    46081 tactgagcga aatgtacatg cctttgtcgg cctggttgtc atactcgtaa cggtagccag
    46141 tcatcgagat gctgacatta cgaatactgc ccatattgaa atagtgcgag agcatgatgt
    46201 tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg ggtatagttg agataaaccg
    46261 aaacacccgc atcgcggaag ttctggttat aagtggcggt gtacatctct ttgtcgttgc
    46321 ccgtgcgtac cattccgctg tcactggcat ccagatactc gctcatggtc ataaagttct
    46381 cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt gagctggtca aagtctttgg
    46441 aataactcac acggaaggag ttaccgtcca gcgaaccttt gccataagcg gtgtctttat
    46501 ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc gccgaatgta gacaaatcgc
    46561 gaccgatgcc aagcgccgca gactgatagt tttcatctcc cagtgcgccg ccatataacg
    46621 accagccgtt agcgatcccc caggaagctt cagcaccaga aaaaaattca ccctcgatat
    46681 ggtgtcccca ctcttgcgga cggcccatca tgatcttata gcgaacctga cctgggcgag
    46741 tgaggtatgg catcgaggcg gtgctgatgt catattcctg cacctggccg ttctgttctt
    46801 caatgcgaat atgcaacgta ccggagacag aatcaccaag atcctgaata cgaaacggtc
    46861 cagccggaac ctgcgtttcg taaatcacac gccccatctg gctgacggtc acttttgctg
    46921 tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg cagattggga ggcaacattt
    46981 gatcgtcagt actgacactg ccaccaacat agttaaagcc atcaaaaata tcggatctga
    47041 ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg taacgcccgc caggcatagt
    47101 agcgactcca ctcccatttt ttttgagttt catcgccgct gaattcatcg tcatcatcat
    47161 tactgcgagt atgttgatag ttagtctgcc agtcagcacg catacgccac ggccccaggt
    47221 taaccccgac cgtcccgttg ccgctgatct cgttactatc atcaccgcca ttttcttcgt
    47281 gccgggtttg tgcgttgatg ctgtagtccg cgacgatccc ggagatgccg tcatcccaac
    47341 gtgaaggcgg atcccagtcg ggataagtat attcgaggta agcctgtggc agtgaaatga
    47401 ctaatgcgga ctggcttaaa tcagccttaa tttccatgcc ttccagttga ccggatttca
    47461 ggcatttagc atcgtggctc cattgcagat ttttcgccac gtcttctttt aaaccaaact
    47521 gcgctaccag ttccggtgtc agacaagcat agcttttgct cgcgtcatct tcaccagcat
    47581 accagtaaat atcgtactct tccgccaatg gctgtttatt tagttgaacc tgtaaattgt
    47641 attttccggg ctcaacatat ccctggctgg aaaaacgctt cagatcaatt tttgtgtcgc
    47701 cttttaattc cagaaaacgg gaatcaaact gaatgtcgtc ttcagcccag acactgctat
    47761 aactccctga cattgccagc gcaatgcacc acggaagaat ccgtaatcga aaaagagaat
    47821 ggtcaggcat acacgtttct atccgttttt attcttgttc ggtcaaaatc catttaaatc
    47881 ccccatagca gtgtcgctat gggggtatgc cttacaggta agtaatctgg aaggtggtat
    47941 ttgcttcgaa attacccaga tctggcgcat cagctgcgcc gaccagccag gctttaaagt
    48001 tcagtgtctg cttcgctttg cctttattgg tagaagtgtc tttcactatt ttctggtcaa
    48061 tacccatgcc gcttttgtaa gaggtaccca gagagtcacc gatcgccagg ctaacattgt
    48121 taaatgccgc accggtatcg gtattgaaaa tggtgtaata attgccgcta tttgcagaag
    48181 aaacggtacc agtaaaggta gtggtcattg tttcctgagt atcaaatacg caatcttgca
    48241 gacgaatctg gaaacttttc ggcgtggtgg tgccgttatt tttcagtttt tcggcaccga
    48301 tatcacccat atcaacttcc agtttgtgat cgtccggaac gatagaacac actgagtctt
    48361 caatggtgcc gtaaaaacgt gcagtaccgt tgttacccgc agcgattgtt gccgtagagg
    48421 tgaaaacgcc catcaccatt gccgcagcaa tagctgtttt aacaaactta ctcattattt
    48481 gtccttaaac tataaataat taaaaatata aacattacaa tacattgatt cagtcaatac
    48541 ccaatgttta tacccagcca ttgaatacgg catgcgatcc aaaaagaaca ggtcgtagaa
    48601 aattgtattc tttggtgtgg ggccgcatag tgatgttttc acccccctga tgcaatttaa
    48661 ttttactaaa gagtaaatgt agatttaatt aatatattga cagaagaaag gttatcttaa
    48721 ggacatcctt aaaaaatacg gaataagtat ttagaaaaaa tagaaagtat atcaataatg
    48781 aatatcaaca gcttaaaaaa caaacaatga acatataatg cgattggcat tt
//
LOCUS       DAZKGS010000016        47633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKGS010000016 DAZKGS010000000
VERSION     DAZKGS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHOQY_000967"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHOQY_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9677113.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHOQY_000968"
     CDS             complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHOQY_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9677114.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHOQY_000969"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHOQY_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9677115.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3208..3750)
                     /gene="ycfP"
                     /locus_tag="ACHOQY_000970"
     CDS             complement(3208..3750)
                     /gene="ycfP"
                     /locus_tag="ACHOQY_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9677116.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3773..4798)
                     /gene="nagZ"
                     /locus_tag="ACHOQY_000971"
     CDS             complement(3773..4798)
                     /gene="nagZ"
                     /locus_tag="ACHOQY_000971"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9677117.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4809..5633)
                     /gene="thiK"
                     /locus_tag="ACHOQY_000972"
     CDS             complement(4809..5633)
                     /gene="thiK"
                     /locus_tag="ACHOQY_000972"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9677118.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5614..6255)
                     /gene="lpoB"
                     /locus_tag="ACHOQY_000973"
     CDS             complement(5614..6255)
                     /gene="lpoB"
                     /locus_tag="ACHOQY_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9677119.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6269..6646)
                     /gene="ycfL"
                     /locus_tag="ACHOQY_000974"
     CDS             complement(6269..6646)
                     /gene="ycfL"
                     /locus_tag="ACHOQY_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9677120.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6649..7008)
                     /gene="hinT"
                     /locus_tag="ACHOQY_000975"
     CDS             complement(6649..7008)
                     /gene="hinT"
                     /locus_tag="ACHOQY_000975"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9677121.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7342..9531
                     /gene="fhuE"
                     /locus_tag="ACHOQY_000976"
     CDS             7342..9531
                     /gene="fhuE"
                     /locus_tag="ACHOQY_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9677122.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9591..11024)
                     /gene="ptsG"
                     /locus_tag="ACHOQY_000977"
     CDS             complement(9591..11024)
                     /gene="ptsG"
                     /locus_tag="ACHOQY_000977"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9677123.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11319..12116)
                     /gene="ycfH"
                     /locus_tag="ACHOQY_000978"
     CDS             complement(11319..12116)
                     /gene="ycfH"
                     /locus_tag="ACHOQY_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9677124.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12127..13131)
                     /gene="holB"
                     /locus_tag="ACHOQY_000979"
     CDS             complement(12127..13131)
                     /gene="holB"
                     /locus_tag="ACHOQY_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9677125.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13128..13769)
                     /gene="tmk"
                     /locus_tag="ACHOQY_000980"
     CDS             complement(13128..13769)
                     /gene="tmk"
                     /locus_tag="ACHOQY_000980"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9677126.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13759..14781)
                     /gene="yceG"
                     /locus_tag="ACHOQY_000981"
     CDS             complement(13759..14781)
                     /gene="yceG"
                     /locus_tag="ACHOQY_000981"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9677127.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14784..15593)
                     /gene="pabC"
                     /locus_tag="ACHOQY_000982"
     CDS             complement(14784..15593)
                     /gene="pabC"
                     /locus_tag="ACHOQY_000982"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9677128.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15713..16954)
                     /gene="fabF"
                     /locus_tag="ACHOQY_000983"
     CDS             complement(15713..16954)
                     /gene="fabF"
                     /locus_tag="ACHOQY_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9677129.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17042..17278)
                     /gene="acpP"
                     /locus_tag="ACHOQY_000984"
     CDS             complement(17042..17278)
                     /gene="acpP"
                     /locus_tag="ACHOQY_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9677130.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17489..18223)
                     /gene="fabG"
                     /locus_tag="ACHOQY_000985"
     CDS             complement(17489..18223)
                     /gene="fabG"
                     /locus_tag="ACHOQY_000985"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9677131.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18236..19165)
                     /gene="fabD"
                     /locus_tag="ACHOQY_000986"
     CDS             complement(18236..19165)
                     /gene="fabD"
                     /locus_tag="ACHOQY_000986"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9677132.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19181..20134)
                     /gene="fabH"
                     /locus_tag="ACHOQY_000987"
     CDS             complement(19181..20134)
                     /gene="fabH"
                     /locus_tag="ACHOQY_000987"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9677133.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20202..21272)
                     /gene="plsX"
                     /locus_tag="ACHOQY_000988"
     CDS             complement(20202..21272)
                     /gene="plsX"
                     /locus_tag="ACHOQY_000988"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9677134.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21353..21526)
                     /gene="rpmF"
                     /locus_tag="ACHOQY_000989"
     CDS             complement(21353..21526)
                     /gene="rpmF"
                     /locus_tag="ACHOQY_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9677135.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21578..22099)
                     /gene="yceD"
                     /locus_tag="ACHOQY_000990"
     CDS             complement(21578..22099)
                     /gene="yceD"
                     /locus_tag="ACHOQY_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9677136.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22298..22882
                     /gene="yceF"
                     /locus_tag="ACHOQY_000991"
     CDS             22298..22882
                     /gene="yceF"
                     /locus_tag="ACHOQY_000991"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9677137.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22994..23953)
                     /gene="rluC"
                     /locus_tag="ACHOQY_000992"
     CDS             complement(22994..23953)
                     /gene="rluC"
                     /locus_tag="ACHOQY_000992"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9677138.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24526..27711
                     /gene="rne"
                     /locus_tag="ACHOQY_000993"
     CDS             24526..27711
                     /gene="rne"
                     /locus_tag="ACHOQY_000993"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9677139.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27907..28860)
                     /gene="flgL"
                     /locus_tag="ACHOQY_000994"
     CDS             complement(27907..28860)
                     /gene="flgL"
                     /locus_tag="ACHOQY_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9677140.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28872..30515)
                     /gene="flgK"
                     /locus_tag="ACHOQY_000995"
     CDS             complement(28872..30515)
                     /gene="flgK"
                     /locus_tag="ACHOQY_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9677141.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30581..31522)
                     /gene="flgJ"
                     /locus_tag="ACHOQY_000996"
     CDS             complement(30581..31522)
                     /gene="flgJ"
                     /locus_tag="ACHOQY_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9677142.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31522..32619)
                     /gene="flgI"
                     /locus_tag="ACHOQY_000997"
     CDS             complement(31522..32619)
                     /gene="flgI"
                     /locus_tag="ACHOQY_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9677143.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSLVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32631..33329)
                     /gene="flgH"
                     /locus_tag="ACHOQY_000998"
     CDS             complement(32631..33329)
                     /gene="flgH"
                     /locus_tag="ACHOQY_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9677144.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33382..34164)
                     /gene="flgG"
                     /locus_tag="ACHOQY_000999"
     CDS             complement(33382..34164)
                     /gene="flgG"
                     /locus_tag="ACHOQY_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9677145.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34302..35057)
                     /gene="flgF"
                     /locus_tag="ACHOQY_001000"
     CDS             complement(34302..35057)
                     /gene="flgF"
                     /locus_tag="ACHOQY_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9677146.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35077..36282)
                     /gene="flgE"
                     /locus_tag="ACHOQY_001001"
     CDS             complement(35077..36282)
                     /gene="flgE"
                     /locus_tag="ACHOQY_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9677147.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36307..37002)
                     /gene="flgD"
                     /locus_tag="ACHOQY_001002"
     CDS             complement(36307..37002)
                     /gene="flgD"
                     /locus_tag="ACHOQY_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9677148.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37014..37418)
                     /gene="flgC"
                     /locus_tag="ACHOQY_001003"
     CDS             complement(37014..37418)
                     /gene="flgC"
                     /locus_tag="ACHOQY_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9677149.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37422..37838)
                     /gene="flgB"
                     /locus_tag="ACHOQY_001004"
     CDS             complement(37422..37838)
                     /gene="flgB"
                     /locus_tag="ACHOQY_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9677150.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37993..38652
                     /gene="flgA"
                     /locus_tag="ACHOQY_001005"
     CDS             37993..38652
                     /gene="flgA"
                     /locus_tag="ACHOQY_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9677151.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38728..39021
                     /gene="flgM"
                     /locus_tag="ACHOQY_001006"
     CDS             38728..39021
                     /gene="flgM"
                     /locus_tag="ACHOQY_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9677152.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39026..39442
                     /gene="flgN"
                     /locus_tag="ACHOQY_001007"
     CDS             39026..39442
                     /gene="flgN"
                     /locus_tag="ACHOQY_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9677153.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39482..41017)
                     /gene="murJ"
                     /locus_tag="ACHOQY_001008"
     CDS             complement(39482..41017)
                     /gene="murJ"
                     /locus_tag="ACHOQY_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9677154.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41127..42050)
                     /gene="yceM"
                     /locus_tag="ACHOQY_001009"
     CDS             complement(41127..42050)
                     /gene="yceM"
                     /locus_tag="ACHOQY_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9677155.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42052..42699)
                     /gene="yceH"
                     /locus_tag="ACHOQY_001010"
     CDS             complement(42052..42699)
                     /gene="yceH"
                     /locus_tag="ACHOQY_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9677156.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42710..43294)
                     /gene="rimJ"
                     /locus_tag="ACHOQY_001011"
     CDS             complement(42710..43294)
                     /gene="rimJ"
                     /locus_tag="ACHOQY_001011"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9677157.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43530..44738
                     /gene="mdtH"
                     /locus_tag="ACHOQY_001012"
     CDS             43530..44738
                     /gene="mdtH"
                     /locus_tag="ACHOQY_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9677158.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44802..45449
                     /gene="grxB"
                     /locus_tag="ACHOQY_001013"
     CDS             44802..45449
                     /gene="grxB"
                     /locus_tag="ACHOQY_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9677159.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45583..46143
                     /gene="yceB"
                     /locus_tag="ACHOQY_001014"
     CDS             45583..46143
                     /gene="yceB"
                     /locus_tag="ACHOQY_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9677160.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46249..47295
                     /gene="pyrC"
                     /locus_tag="ACHOQY_001015"
     CDS             46249..47295
                     /gene="pyrC"
                     /locus_tag="ACHOQY_001015"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9677161.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47369..47614
                     /gene="dinI"
                     /locus_tag="ACHOQY_001016"
     CDS             47369..47614
                     /gene="dinI"
                     /locus_tag="ACHOQY_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9677162.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11518 a        12900 c        11847 g        11368 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc gccaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 tgattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccat tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt
     2881 tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc
     2941 aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc gggtgagatc
     3001 aaaaaacaga gtttattgtt tattatttga gcaagtccgg aagggcgata tagggtgaag
     3061 cgtatatcga gggcgtttgg tcgtcctgat aagaggcgca tcaggcattg atgctcgaat
     3121 gtcggatgcg gcgtgaacgc cttatccgac ccacagaatc cggagcgcat caggtaccgg
     3181 caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt taatgcgctg
     3241 taaatgcggg gagatattct tgaatttgtg cgtctgctct tcgtcccaga caatctcgta
     3301 ataatgatgc agctcttcag atgtccgctg gctgttaagc gcttcatcat tacgcgacaa
     3361 aatcaccagg caacgatcgc gattcttctc acggaagttg gtcacacact tagtggcaat
     3421 atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag ggaacaaatt
     3481 agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg cccagtatcc
     3541 gcccagacca acgccgcaaa tcagcggacg ctcgtcgacg ttcagttgca acattttgtc
     3601 cacttctttg agcagatgct gcatatcatg tttcggatgc cgcgtactgt agcttatcaa
     3661 gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac ccggactgtt
     3721 agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag ggttagtgac
     3781 ctgctttctc ttcctgccag cgttcatgta actgattcag acgggtgctg atcgctttcc
     3841 agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa
     3901 cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct ttacgattat
     3961 tgcagaccag gatcatatcg caacccgcat ccagtgatgc ctgcccgcgt tcggcataac
     4021 tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac
     4081 ccagttcctg acgcaaaacg gttttcagcc agtagggaga accgctcgcc ggacgcggat
     4141 caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt tcgcggatta
     4201 aggaactgaa gaccgacata tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg
     4261 gtgtttcttt gtgtgagtct gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg
     4321 ttttcattcc ggcttcatgc ataccatcaa taaaccggct ggcaattgcc agggcttttt
     4381 gtggatcggc atgataagaa cgctcgccaa tcgccgcgct gatatgcccg acatccagca
     4441 ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc agccaacccg
     4501 cctcttgcgc cagtttgccg ccctcttcca ttcctgacag cgcagcgaat gattgcgccg
     4561 ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg tccaccttcc tgatctaccg
     4621 ccaccaccag atgattgcgc gaagctgcgc ggatctggcg caccagttca cgcaactggg
     4681 caggatcatg atagttacgc gtaaagagaa tcagccctcc caccagcgga tgcgcaagta
     4741 tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact ggacccacac
     4801 tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag cctgataaat
     4861 tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag catcagcagc
     4921 cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt cgcgcgagtg
     4981 gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac tgccgccagt
     5041 tccagcgcga tatcaccatc tccggcatac tcccagtcga tgagttttaa ccctgacgcg
     5101 ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg taaaggccgt
     5161 ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt ccgccgcgcc
     5221 ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg ccagccaaaa
     5281 cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc cggcaaatac
     5341 gtttttacct cgccgggcag atagtcgact accatccagt cacggagata taaatgcggc
     5401 ttcggtgcaa tgcttgcggg tagttgtgat aaagcccgat actggcgcaa gaacgcggac
     5461 tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg atgttcaatg
     5521 agaaaactcc cgccactaag cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa
     5581 aagcgcgaca gcaattcgtc gcgcatgatg ggattattgc tgcgaaacgg cacctttacc
     5641 tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg tcggagcgtt
     5701 aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc gggcaatgcc
     5761 tatggcttta ctacgggtac ctaaactgtc ctgcggcgac aaacctaact gttgcttagc
     5821 catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca gcgcatttcg
     5881 cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt taacgctatc
     5941 aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac tgaccatcgg
     6001 ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg ccgtttgatc
     6061 ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca ctgtggggac
     6121 aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta ccggtgcagg
     6181 ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca aggcgtagcg
     6241 actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg actttgtgcg
     6301 cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga atggtaacgc
     6361 tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag taaaaacgat
     6421 aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct gaaggttgaa
     6481 tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat aaagatgact
     6541 ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga cagcccacca
     6601 gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt acagaccttt
     6661 atgtgccagc attggtccca gcggacggcc acccaacaag tgcatatgga tgtggtaaac
     6721 ctcttgtccg ccatggcggt tggtattcat gatcagacga tagccatctt cggcaatacc
     6781 ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct gctcatgctc
     6841 agctgacacg tcgttcacag tcggaatgag gatattcgga atgatcagaa tatgcgttgg
     6901 cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga cgatatcgga
     6961 ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga cattttcctt
     7021 tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca cactgcgagt atagagtatg
     7081 gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa aaatcgtttt
     7141 gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga aaagacattc
     7201 tctgtttggt ttacacgtca aatatggatg cgtatatttc tcatttgcat ttacatacaa
     7261 aattattcgc acactaaata taaccgctca tcaccctcct ccggcatgag cctgacgaca
     7321 acataaacca agagatttca aatgctttca acacaattta acagggataa tcaatatcaa
     7381 gccagcacca aaccatcact acttgccggt tgcatagcat tggcactatt accttctgcc
     7441 gcttttgccg ctccagccac tgaagaaacg gtgattgttg agggttcagc cacagctcca
     7501 gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa aatgcagatg
     7561 actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat ggaagatcag
     7621 cagttacaaa cgctgggcga agtgatggaa aacacgctgg ggatcagcaa aagtcaggcg
     7681 gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta tatggttgat
     7741 ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc tgatatggca
     7801 ctctttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg gacgggtaat
     7861 ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt taaaggcgat
     7921 gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt acaaagccca
     7981 ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa taacgactca
     8041 tggctggacc gctacaacag tgaaaagacc ttcttctccg gcattgtcga tgctgattta
     8101 ggcgatctta cgacgctttc agccggttac gaatatcagc acattgatgt taatagccca
     8161 acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta cgatcgcgca
     8221 cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt ctttatgacc
     8281 ctgaagcagc ggtttgctga tacctggcaa gcgacgctga atgccaccca ctctgaagtc
     8341 gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga tggtatgctg
     8401 attgggccat acagtaatta tggacctggc tttgattatg tcggcggcac cggttggaac
     8461 agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtagtta tgaattgttt
     8521 ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa tcgttacttc
     8581 agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt taatggcaat
     8641 ttcccacaaa ccgactggtc accacagagc ctggcgcagg acgataccac acatattaaa
     8701 tcgttatatg ctgccactcg tgtcaccctt gccgatccgc tgcatctgat cctcggcgca
     8761 cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa ccacaccacg
     8821 ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc cagctatacc
     8881 tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc tccaatcacc
     8941 ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct gaccaccacg
     9001 ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac acctatcccc
     9061 ggcagcaacg gcgaaaccgc ctataaagca gtggatggga cagtcagtaa aggggtggaa
     9121 tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc aacgcgctat
     9181 attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac cacggttaaa
     9241 atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg tggtgttaac
     9301 tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg cgccgagcaa
     9361 ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa cttctcgtta
     9421 caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga aggttctatc
     9481 gtctacggcg caccgcgtaa tttcagcatt accggcacgt atcaattctg aatagcacct
     9541 gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacaga ttagtggtta
     9601 cggatgtact catccatctc ggttttcagg ttgtcggatt tagtaccgaa aatcgcctga
     9661 acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc agcctgatcc
     9721 actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc gaggttagta
     9781 atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct ggtacctgtc
     9841 gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag tgctttaatc
     9901 agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat cgggaacagc
     9961 cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg cgagaacgac
    10021 gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc cagaatcgcg
    10081 tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg ctcggtgata
    10141 ccggtcagga acgaggtcag cgccgcggag atcataatac cgcccacttt cgcgcggttt
    10201 tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta cattttgaac
    10261 aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg cggaatgtcg
    10321 ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg gaaaggtacg
    10381 ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc gtaaatgcca
    10441 aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat tgcagaacca
    10501 atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag gccagaaatg
    10561 atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt aatacggtag
    10621 aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccctccgag tacgccagta
    10681 tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag tggcgcaacc
    10741 acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc ggatacgcca
    10801 tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat gtttgcaaag
    10861 acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca gctgaaattc
    10921 gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg cagcatcagc
    10981 gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga gagtgctcct
    11041 gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc tcgccgtgta
    11101 cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt gccaatttgt
    11161 ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt tgacaaaaca
    11221 tatgtcacaa tatttcaaac gtggttattc accgcccaat tttcggcggt gaactttact
    11281 cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga ttgaaggcgg
    11341 gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc cagttcttca
    11401 acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat cgcaggttga
    11461 ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc caccagtaat
    11521 cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg gaaggtcaca
    11581 atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt ttctctgtcc
    11641 tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg cagaattgcc
    11701 agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag ttcacgacca
    11761 atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg tgtgtaataa
    11821 taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag acggcgtaaa
    11881 tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga aaatacgacg
    11941 ttgtcacgtt cgcctaccag atcccgcata tgtaaataac ccggtaatgt tgtggcgact
    12001 gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc cacgtcctta
    12061 tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa aaacatgatg
    12121 tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat gctcaatacg
    12181 cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtaacagaca ttaactgttc
    12241 acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa gatggtttgc
    12301 cagttcggcg accaggcccg gcacatcaac attggtcacc tgcgcagcac catgatggcg
    12361 ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag cttgttcatg
    12421 attaagggcc gctagcagcg aataccagtc gcccgatggc acgctatatg ccaacgcctg
    12481 acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg ccgcgccagg
    12541 cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg tcacttcgcg
    12601 tgaaagccag gtcacggcgt actgttccgg cggcggcgca aggtaatgta accgacaacg
    12661 actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa accaggtttc
    12721 tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg tcagtaaggc
    12781 agcatcggtt acccagacaa ctttcgcacc acctaggcgt gcgtgctcat tcagcttttc
    12841 ggtgacctca cgtaccgcat caatgcccag catatttttc cctttttcgg gagccagggt
    12901 gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt gaccgcaact
    12961 tttgtggccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga ttaaagcatc
    13021 atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc cggcctgata
    13081 gctggctacc agtttttcga aatcaggtcg taaccatgga taccatctca tgcgtccaac
    13141 tccttaaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc cagcggctgg
    13201 gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata gcgggcgcgg
    13261 gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc gcgcgcacgc
    13321 gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc ggggcgaaaa
    13381 tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc acgtcctccg
    13441 ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa tcacccaggt gccgttagcc
    13501 agagctggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa catcagaact
    13561 tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac caggcttctt
    13621 aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc gcggataccc
    13681 agttgctcaa gcgtctcaac caccacatta cgcgcggtag ttttgcctgc gccttccagc
    13741 ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca gataatcctg
    13801 cacagacttg ttatggctgg caagattggt attaaacgtg tgaccacctt taccatcggc
    13861 cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg aatccgcccc
    13921 cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat acgctgtcgg
    13981 cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc cgtaaatcac
    14041 ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata ccgaggcaac
    14101 ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca tcgtcaccag
    14161 ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc tatcgaccgc
    14221 tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat tggcggtata
    14281 catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca gttcaagtgc
    14341 ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg gagcctcacg
    14401 caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg ggaactgtgc
    14461 ttctttaccg ctttccaaca atttcagcat ctcgcgcacg gtcatctgcg gtgtaaagcg
    14521 gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcagcc attgaaaaac
    14581 ccgtgggcga ttgatgatct tatcagcata aagctgttca ccgagcgcca gacgtccggt
    14641 ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt cggcaagatg
    14701 gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca ataacaagat
    14761 aatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc taaatattca
    14821 tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat cactggcatt
    14881 aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga ggcttgcact
    14941 tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg cataatgccg
    15001 ttaacacctg cctgatccag tcgcggcgta taaactacat tgcccttccg ccagaacaaa
    15061 ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc ctcatcagcg
    15121 tttgtctgct caagatgaga gcggatcaat acttgctcaa gccgattaag atgtttaata
    15181 cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt catcccttcg
    15241 ttacgcaaac ggtcgtaatg tgcaggataa accgtaacgg agagaatccg cgttgccggt
    15301 ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac gactttcagc
    15361 acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg aggccagaag
    15421 tcacagaaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg cgccgataac
    15481 aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc aaactgcgtt
    15541 gcccgatcgc ttactgccag cgattcctgc ttataaccgt taattaagaa cataccggct
    15601 ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc ggaataaaag
    15661 ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg gaaaatgaca acttagatct
    15721 ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac agcgtgtatt
    15781 ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa ccttcatccg
    15841 ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg atggagtaga
    15901 tagattctac tgcacccgcc gcacctaaca ggtgaccggt catagatttc gtggagctta
    15961 ccaacacacg gcttgcagct tcaccgaaga tggttttcac cgcctgcgct tcagctttat
    16021 cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc aatctgactc gcttcaatgc
    16081 ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt tctggcggtg
    16141 acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg taaattttcg
    16201 caccgcgttt tttcgcgtgt tcgtactctt caagtaccag cataccggca ccatcgccca
    16261 gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg ttatcattgc
    16321 gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc cagcggcgta ctggctttct
    16381 ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc gcagcatgac
    16441 caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg ccacgcaggc
    16501 catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga acgaagaatg
    16561 ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg atcagtccga
    16621 ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct tccgttattt
    16681 caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga atgaaggcat
    16741 ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc tttactaagc
    16801 cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg atgccactct
    16861 gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga gacaacatgc
    16921 ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga gggaaaaaat
    16981 gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg gagatgttca
    17041 cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg attttctcag
    17101 cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc agctcaacgg
    17161 tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta acttcttcct
    17221 gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata gtgctcatac
    17281 tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat aaaatgttga
    17341 aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt tgaggcctta
    17401 ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc agactgcgcg
    17461 caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg ttcacatgca
    17521 aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc gcgttggcga
    17581 tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc gcacgctggt
    17641 catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca acgtttacag
    17701 taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc aagcccgctt
    17761 tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca gaaccgatag
    17821 tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt gacagacgga
    17881 aaacagatga aaggttggtt tcgataattt cgttccactc ttcatctttc attcgcatta
    17941 acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca ccaaattctg
    18001 cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc aacatcagac
    18061 ctttgccgtt ggcacctaaa taatcgctga tcgcctgagc gccattttca ctggtcgcag
    18121 tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg ccaattccgc
    18181 ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc ctcttttaaa
    18241 gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc agggtgtcga
    18301 caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc gacttcatag agatgttcta
    18361 cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta tacaactgac
    18421 gtaccagtgc gtcacggatg gcatcaccat tggtttcgca tttcacatca acgttattca
    18481 caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc agtttgtcgg
    18541 ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc agcgcgcgtt
    18601 ttgcgccagc agctttacag gcagcgccag cacgctcaac cgcttcttta tgaccggcaa
    18661 taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct tctgcagctt
    18721 cttcacacgc tttcgcaata gacgcatcat ccagaccgat gattgccgcc atagcgccag
    18781 tgccttccgg tacggcttct tgcatgaact taccgcgcat ctcaaccaga cgcaccgcat
    18841 cagcgaaatt aatcacgcca gcgcaaacca gggcggagta ttcccccagg ctgtgaccgg
    18901 ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc gcaacagatg
    18961 caatcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct ggcccctgct
    19021 gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac gtttcttcga
    19081 caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa ccctgtccag
    19141 ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga accagcgcgg
    19201 agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc ggcttaatgc
    19261 gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag gtattaccgt
    19321 ggcgatccag cgtcaccacg acattgtcca tagacatacc gagttttttc gccgttgcac
    19381 tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa cggtcaagat
    19441 tatttgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca accttgaaga
    19501 cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacacgg tcagcattag
    19561 gcagcgtcag caactcacca tagctaccgt cggcatgcag atgggtggag atgattcccg
    19621 gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata ataatagtcc
    19681 cacgatcggt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc agagcatact
    19741 tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg aaacctgcgc
    19801 aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc caacatgctt tgaatctgac
    19861 aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc aggccaatct
    19921 ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag cccatggttg
    19981 aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga gtgacaatcc
    20041 actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact tgttcgggca
    20101 gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt ttcggttata
    20161 taccgtcact tgcaaactgc gagttcgctg gcagcgtcct gctaccgcag agttccgctt
    20221 ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg agcggcaatt
    20281 cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac cgcaaaagct
    20341 cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa cagacaggcg
    20401 ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt ttgtagccaa
    20461 cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga tttcagcaga
    20521 gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc tgtaaagccg
    20581 tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag atagccgata
    20641 taattgatag aagggattgt tttaagcacc gctgaggcat cccgaatact gtcgagaccc
    20701 tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat ttccaccacc
    20761 tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct gtcacaatcg
    20821 acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg taataccgtc
    20881 accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc cagccccatc
    20941 agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt caccagctcc
    21001 agggccatac gcattgaact cccacgactg gcgcggatag cttgcgaagg ccgggcatca
    21061 ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc aaagtcagct
    21121 ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt gagttgcgaa
    21181 ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa atcccctccc
    21241 atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg tttggtcttt
    21301 ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt gattacttag
    21361 cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc aggtgttttt
    21421 caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca tgggaacgac
    21481 gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac cttactcctc
    21541 aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt aagctggcta
    21601 atacggcaaa tgggtttggc ttttgcgctt cttcaggcag ttcaccaaag accatgtccg
    21661 cttcggacac ttcacagtgt tcagaatcat gcaccggaac taccggcaag gcgaggatga
    21721 tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc tcaatcggtt
    21781 catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta aaacaatacg
    21841 ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc gttaccgtca
    21901 ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac gacatggagc
    21961 attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc tgatcagggg
    22021 tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg agagtcaggg
    22081 gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata gagtcaaaga
    22141 aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg ttactgcttt
    22201 attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat agtttaaaat
    22261 ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta ttttagcctc
    22321 cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg aatgtgcagc
    22381 accagaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg tgcttcgact
    22441 ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa ttattggttc
    22501 agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg aagaaaatgc
    22561 ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg gactggcgtt
    22621 gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg tccatttccg
    22681 tcacctgagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc tgcactgcgc
    22741 gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag agggacgtga
    22801 ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac gtcgggaagg
    22861 gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc atccggcaat
    22921 cggtgcaggc ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc attatcaggc
    22981 ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc aaaccttcat
    23041 ccatcggcgc ttcgatacgc atcacctcac cggtccccgg atgggtaaac ttcaacgctg
    23101 cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt ctgtcaaatt
    23161 cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt gtatgcacac
    23221 ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca aatgcatagc
    23281 gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca cgcacgatac
    23341 gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc acatgcgact
    23401 gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca cgtaattgct
    23461 catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct gaagtgtccc
    23521 ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc cgcaaacctt
    23581 cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa ggtttattca
    23641 gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc accttttgca
    23701 gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg atacgcacct
    23761 catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc cgcacttcac
    23821 ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg gtacgcaaaa
    23881 agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt acggatggag
    23941 tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca ctcatttttc
    24001 tatgcttata tttagtttta caccttatta cttcactacg tgatcacttt attgatggtt
    24061 attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga taaatggtaa
    24121 aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc gtgtccatcc
    24181 ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc
    24241 aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac
    24301 cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg
    24361 tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg
    24421 tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt
    24481 tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta
    24541 atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat
    24601 gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa
    24661 atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac
    24721 ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat
    24781 ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat
    24841 aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc
    24901 tatctggttc tgatgccgaa caacccgcgc gcgggtggca tttctcgccg tatcgaaggc
    24961 gacgaccgta ccgaattaaa agaagcactg gcgagccttg aactgccgga aggcatgggg
    25021 cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc
    25081 ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc ggctccgttc
    25141 ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt acgtcaggac
    25201 atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct
    25261 gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg
    25321 ttcagccact accagatcga gtcgcagatc gaatccgcct tccagcgtga agtccgtctg
    25381 ccgtccggtg gttccattgt tatcgacagc accgaagcgt taaccgccat cgacatcaac
    25441 tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac caacctcgaa
    25501 gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc
    25561 gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa
    25621 gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg
    25681 gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat ccagtcatca cgtttgtccg
    25741 cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt
    25801 ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgtg
    25861 ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgccat tgaaactcgt
    25921 caggacggtg tgcgctgtgt aattgtgcca aacgatcaga tggaaacccc gcactaccac
    25981 gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct gccgaagctg
    26041 catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa
    26101 caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcgccaac gccagctgaa
    26161 cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac cggcaacacc agcaactcct
    26221 gcacaacctg ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt cagcggtagt
    26281 gaagaaacca aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa
    26341 caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg ccgcgacacc
    26401 cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag
    26461 gcacagcagc agactgccga gacgcgtgag agccgtcagc aagctgaggt aacggaaaaa
    26521 gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat
    26581 gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag
    26641 gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc
    26701 aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt
    26761 gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg
    26821 gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat
    26881 gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg
    26941 cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca
    27001 atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc
    27061 tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta
    27121 caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc tgttgttagc
    27181 gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg
    27241 caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag
    27301 ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga acacgcagaa
    27361 ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag
    27421 gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc
    27481 gcagcagaag ttgaaacggt agccgcggtt gaaccagaga tcaccgttga gcataaccac
    27541 gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcgtcac
    27601 agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg
    27661 gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct
    27721 caaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg aatcaacata
    27781 ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaatgcc
    27841 cgccccggcg atcccagagc aggcagacaa aacataccca gtttcatgat atgttttaaa
    27901 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    27961 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    28021 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaatgaatc
    28081 cagcgactcc agttcgttca gctgcgtgcc taattccgcg cgaactgtaa ggacattatt
    28141 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    28201 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    28261 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    28321 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgcct
    28381 aatactttct gctccaccga cgtatttccc tttctcttcg ctaaacggcg cagtctctgt
    28441 tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg gtgttcgcca gattcagcaa
    28501 ctggtcacga agcccctgga tatccgtagc cagcgaggcc cggtcatcgt cactcaaagt
    28561 gccattgctg gcgtagacaa ttttttcctg agcattctgg atagcagtgg tgacctggct
    28621 aagtacactc tcttccagtg acactttttg agtggcgaaa gtacgcgcca gcgtgtactg
    28681 gctgttttgc gcctgtgcct gggagagaac aaccgcttgt gatgctgcaa tgggatcgtc
    28741 agaagggtta acgactcgct tacctgtcga catctgctcg ccgtacttca tccattccgc
    28801 ctgagaattg gtgatgccac gcatgttttg ctggtacatc atctgggtac tgaaacgcat
    28861 cttatctccc cttagcgaat gttaatcagc gcatcaaaaa tcgcgtttgc cgtctgcaga
    28921 acctgcgcat tcgccaggta atactgctga aaacgttgca gatttccgta ctcctcatcg
    28981 agattgacac cggaaatcga ctgctgctga ttggaaagct gcgtcaccac attaccttgc
    29041 gtggcgctac tggttttcaa cgtcgcggtt ttattaccga tatcactcac taacgaggca
    29101 taagcgtcgt taaaggattt cgcaccgccc accgttttac tgttgctttg cagatccagc
    29161 agggcctgac cgttgcggtt atcgctatca cccgcatctt cttcgctcgc catcgctatt
    29221 ttcgcttcgt cggtgattaa tacatccatg ttgacgatag cgtcacttac tggtttcagc
    29281 gtgaagctgt cgttaacagc aggcgttcct gtaaacgtca actccagacc atcaaatgcc
    29341 actttaccgt tggcatccgg cgtcaccgta aaagtggtat tactggcaag gcgggtgacc
    29401 tgccactgat tattatcgaa cgagattttg taatcagtcg ccagtaccgc ggaggcatca
    29461 gttaccgtgg caccgatcgc aacgtcacct gtgtttttcg tgttttgcag aaccgcgggc
    29521 ttaccgatag caaagaaatc ttcaccagca tcgccgttgg catcaaatcc ggctttgtgt
    29581 tggctgttga aagcctcggc aaatgccagc gccagttgtc caagcgtatt acgcgtctgg
    29641 tccagatcct gagaacggaa tgtcagaatg ccgcccagcg acccggtatt cagtaatttc
    29701 tccgggatct caatattgcc tgccgtccca tcaacgtaag cgacagtcgt acgagaaggg
    29761 tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac ttccctgaac cagtgagtaa
    29821 ccattggcca tcgtgatgtt ataagtaccg ccatcctgaa cgctgacttc tacgccaaca
    29881 atctggttta attcgctcac cagttgatcg cgttgatcca gcagattgtt aggtgacgcc
    29941 cctgccccca cgcctgtcag gcgcgagatt tgatcgttca ggctggcaat ttgtttagcg
    30001 taattgttga tctgatcaac gctggcacct atcgcgatat tgacctgttt gtcctggtcg
    30061 cgcagatatt gatcggtggt tttaaactga ttcaccaacc cttctgattt cccaatcagc
    30121 gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg tttgcaggct ggtgaagaaa
    30181 tcctgcatct gtgttgccag cgaagaggta ctggtggaga gcatattgtc gattttcgac
    30241 atctgctcat agcgggcagt caggccgcta ctttgcgtct gcgccgcacg taactggttg
    30301 gtaataaaag catcatactc acgctgcaca ccagaaacgt agacaccatt gccaacccag
    30361 ccgccagcgc ccaacgtgct attggcctgc gccataatag tggtttggcg ggtatatccg
    30421 gcaacgttat agctggagat attattactt gccgtattta acgccgcctg ggccgcgttc
    30481 agtccgctca tggcgttatt gatcaagctg gacatggagg ttccttataa gccttcaatt
    30541 acagagtatt atcggcagcg acccgccgga cttgagttat tcagaacaga ttatcaatgt
    30601 tcatgctgta ggttttgctc accttgtcgc ttatcgattt catctgctgg atcatgttgg
    30661 tgagtttacg ggcatagtga ggatcggtag cataccccgc gtcctgtagg gcctgcgccc
    30721 cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg cgggttacgt gttaacagcc
    30781 caacgtaatc cgacaatgct tccagatacg agctgtagac ccgaaacttc gcttttactt
    30841 tcttcgcttc gccgttttca tattcagtcg tggtgatttc agtaactggc cctttccagt
    30901 tgccagaggc tttgacacca aacaggttat agctcggctc gccgttttcg cggcggattt
    30961 gccgttgccc ccaaccagat tccagtgccg cctgagcgag gatcaaatga tgtggcacac
    31021 cgctttgctg gcttgccagt tgggcgggca gcgagagttg cgcgaggaat gctttactgt
    31081 cacccggcag cgaatcatcg tagttacgtg gcacggcctt ttgcaccagc tgcgaaagcg
    31141 cctgattttg ataacgcacc acagtttcga gcgggaattt catcggtgct gctggcgtgg
    31201 actcctctgg caatggttgt tctggcgtca tctgtttaac catcatctct gcaagcccca
    31261 gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc atacatactg gtatacaggc
    31321 gagtgtgctc gctgctgaac aggccatctt ttggtaaagc gtcgcgcatg cttttcaaca
    31381 tcatctgcac gaacatccct tccacctgac gggccaccgg acggatattt gccgccggat
    31441 cttcgcccgc tttcgccttt agttcgttga gtgattgcgc atcccaggcc gcacttgcca
    31501 gtagtttgct gtcgctgatc atcagatgat ttccagtttt gcccgcagac atcccgcact
    31561 ttgcattgat tgcagtatgg acatcagatc catcggcgta gcgcccagcg cattgagcgc
    31621 gcgcaccacg ttattgaggc tggcgctgga acgtacgctt tgcagcgaac cgccgctctg
    31681 gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt ccaccaccaa acggtgtatc
    31741 tggctggctg acattggcct gacgattgac tgttactgag agatttccct gcgctaccgc
    31801 gcagctgtcg agggtcactt cgcgattcat caccaccgaa ccggtgcgcg agttaatcac
    31861 tactttagcg tcctgcgggg tgacattaac ctgcatattc tggatatctg caaggaaacg
    31921 gaccagggaa ctgttgccac ttggtacgcg cacctgaata gtccgcgcat ctaacgcggt
    31981 ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg atttgctgcg ccatgctgaa
    32041 atcttcgtcg ttaagttgca aattaagggt attcccgacg ccaaactggc tgggcaattc
    32101 acgttcaata accgcaccat tggtgatccg tccaccgttc agttggttca cctgaacact
    32161 gctaccgcca gcggaggctc ctgcgccgcc aaccagaata ttgccctgcg ccagcgcata
    32221 cacctgactg tcaacgccct taagcggcgt catcaacaac gtacctccac gcaagctttt
    32281 ggcatttccc atggaagaaa ccaccacatc gatggtttgc ccctgacgtc caaacggagg
    32341 aagtgacgct gtcaccatta ccgcagcgac gttttttagc tgcatattgg tgcccgtcgg
    32401 aacggtaatt cccagctgtg agagcatgtt attaagcgtt tgtgtggtaa acggcgtctg
    32461 ggttgtctgg tcaccggtgc catccagccc caccaccaga ccatagccaa tcagtgagtt
    32521 ttgccttacc ccctgaacac tggtgagatc gcgaatacgc tcagcctgag ccgccgtcgt
    32581 aaccagtaga agaattaatg cagagagaaa tttaatcacc acagcctcac ttacattggc
    32641 gacaggttaa ggaagaaacg ctgcaaccag cccatatttt gcgcttcgtt aatgtagcca
    32701 ttgcctacgt attcaatgcg cgcatccgcc acctgagtag acggtacggt attgctgccg
    32761 ctgatagtgc gtggattaac cacgccagag aagcgaataa attcggtacc ctgattaatg
    32821 gcaatctgtt tttcacccac cacatgcagg ttgccgttga ccagtacctg gtcaaccgtc
    32881 accgtcaacg tgccgctaaa ggtattgctg gcattggccc cgccctttcc gttgaacgtg
    32941 ttaccaccgg aggcttcgac atcggcacga gcgttaccaa acagcccctg caaatagcgc
    33001 ggcacagtat caaagccaaa attagtttta ccgtcacggc tggcattcgc agaggagctt
    33061 ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg tatcgccaat attgcgtggt
    33121 cgacgatctt caaacagcgg ttgatagcca tagttaatcg gctgagcaga ctggaaaata
    33181 gaaccgttgg cgacgggcgt cggcccggga accggttgtg cactggtcgc cccctgcacc
    33241 agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa gcaccaacaa gctggaaatg
    33301 gcataagtat gcgcagcgtt tttttgcatg gctatcatct tcaaaaatca gtaaaccggt
    33361 gaacctgcca ccggttaagc cttagagttg cgtcagtttt tgcagcatct gatcggtggt
    33421 ggacaccgct ttactgttga tttcgtaagc gcgttgcacc tgaatcatat tgaccagttc
    33481 ttccgccacg ttgacgttag acgtttcaac atacccttga tacagcagtc ccgcgccgtt
    33541 caggcccggc gtgctttcgt tcggtgcacc agaggattgc gtttcggtgt agaggttttc
    33601 gccaatgctc tccagcccgg tgtcattcat aaaggtggtg agattgagct gcccaacctg
    33661 aaccggagct gcctggcctt gttgggttac gctgaccacg ccatcacgac cgatggtgat
    33721 acttaacgca ttcgccggaa tggtgatcgc tggctgcacc tgaaaaccac cagccgtcac
    33781 cagctgcccg ttctgatcca cctggaaaga gccgtcacgg gtataggctg atgaaccatc
    33841 tggcaacatc acctggaaaa agccctgccc tttaatcgcg acatctttgc tgttgttggt
    33901 ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg accgggcgta cccccgtgcc
    33961 gatttgtaat ccggagggta aggtggtttg ttcggaagac tgtgcccccg gctggcgaat
    34021 ggtttgataa agcagatctt caaacaccgc gcgctgacgc ttaaaaccgt tagtactgac
    34081 gtttgccagg ttgttggcaa tgacgtccat attggtttgc tgggcgtcaa ggcccgtttt
    34141 ggcgatccat aatgaactga tcataaaatg tcctgtagtg gattagtgac aagcagagca
    34201 gaagcacgcg acagtgtgaa tttcccctca ccctaaccct ctccccaaag gggcgaggga
    34261 atagtccgcg cgcttttatt gcaatgtcat caatcgttca attaactcat cgacagcagt
    34321 tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa
    34381 cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc
    34441 agaacccccg acatcacacg caaggttgga tctgcctgca gtatcggccc acgcgtggcc
    34501 tggctttctg cgcttaaacg aaaaatgccg tcgtcaccgc gttgcacttc gctgcccgtg
    34561 gctttcacca gtttcagacg ccccactggc gcaaccgtat ttgccggatc gcccggattg
    34621 agcgccgaga ttgtgccatc ggcagcaata gtgatttccg ccccttccgg cacagcaatt
    34681 ggcccagcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt gggatcaacc
    34741 tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggactgcac ggccagccag
    34801 ccatcctgct gcaatgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc
    34861 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg tgggcagtga aagcccttcc
    34921 accggcactg cacgcaaggc gtttaactgc gcgcgaaaac cgggcgtaga ggcattggcc
    34981 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcggta
    35041 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt
    35101 gaggatctgg tcctgggttt tgatggtctg ggcgttagac tgatagttac gctgggcaac
    35161 gatcatattg accagttctt tactgagatc gacgttggac gcttccaacg caccgttggt
    35221 cagggtgcca aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    35281 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    35341 tacaatctgc cccagcagtt gtgtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    35401 atcattgatt tgataactca ccagatcgcc cggtttgtag ccattctggg tggttgccac
    35461 aatattgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc
    35521 gggatctgcg ccgttaattg cgccagtggt aatattctct gttggatttg aggtcagaac
    35581 gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct
    35641 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc
    35701 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    35761 attgctggcg ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg
    35821 catcgatgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt
    35881 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac ccgtcagctg
    35941 taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct
    36001 gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    36061 gtccagacct cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    36121 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    36181 tgatttaaag ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    36241 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    36301 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    36361 cagcgtatta ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    36421 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg
    36481 cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtgaactc cggcggtcag
    36541 ttcaccaata tcaatggtgc gcacaaccgc accattttta tcggtgatgg tggccgtaac
    36601 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagctccttc
    36661 ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccgatcag
    36721 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagtgtggt
    36781 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    36841 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa
    36901 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    36961 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    37021 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacgttggcc
    37081 tgatagctgc gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc
    37141 atttttacgt agccctttgc atctgccagc ggattacccg gttcataaac cagtttgtcc
    37201 ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    37261 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    37321 ctatcagcat ttgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    37381 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    37441 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    37501 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    37561 ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccggg
    37621 atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc
    37681 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatcg
    37741 gcattggcga tgtttgctgc cagcacttcc tgacgctggg cgcgcagatt gagcgcctct
    37801 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    37861 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    37921 gtcgctattt atgccgttga tggtcattgc ggacaggtac aattcacgtt gtagaaatgg
    37981 ctgggggcga aaatgctgac aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    38041 ccgttaagta cggcgagcaa tctcacatcg caattgcaca acttttttag cgcccaactc
    38101 gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag cgcccaatct actaccgcca
    38161 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    38221 ttggcacgct gcggtaacga caaacgatat ttacaggtta atgtgcaggc cacaggaaat
    38281 tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    38341 ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    38401 gttgatgcca ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    38461 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    38521 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    38581 gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    38641 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    38701 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct
    38761 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag ccgggcggca
    38821 aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    38881 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    38941 aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag
    39001 caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc
    39061 cgctgtgctt aacgatctca aaacggtaat ggatcaagag cagcaacatc tctctatggg
    39121 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac
    39181 gctggattac ctcgaacagt tacgcaagaa agaacccaat acagcaaata gcgttgatat
    39241 tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    39301 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    39361 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg
    39421 cggtaaaaag atttcgatct gaatattatc tccggcctgc actgcaggcc ggaatgcatt
    39481 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    39541 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagtaagcgc cagggcatgg
    39601 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    39661 gtaccgccac caccagacgc aacagaaacg ccatccagcc gggttgcgga gtaaagattt
    39721 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    39781 aaagtgacag cccggcatgt ttcaacggac caataaacgc caggttcatc aattgcgtta
    39841 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    39901 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    39961 cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg
    40021 ttaacggacc agaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa
    40081 gacgcaaccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    40141 tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    40201 ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa
    40261 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca
    40321 tctgtttcac cacgcgcatt gttccggcat cgtggaagtt aatgcgcggc aggaccagca
    40381 tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggatgccg ccgaccgtaa
    40441 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    40501 caatcatgct gatgttaagc agtgttggag caaacgccgg aatcgagaag cggttccacg
    40561 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    40621 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    40681 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcga
    40741 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    40801 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    40861 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    40921 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    40981 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    41041 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    41101 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    41161 acgatacgtt gcgccagcac ggcctgttcg ccggcggttt gcggaactgt ctgattttgc
    41221 acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgttcaag agtgctctgc
    41281 caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata
    41341 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    41401 gcacggcgat gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    41461 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    41521 cccgacaacc acagcacggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    41581 agatcgtgtg gcccgacact attgctacga tgtttatcca ttcttagcga agctgcggta
    41641 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    41701 aacgtcagtt ttttacgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    41761 gccagcggtt tatcgacaca gacatgtaca cccgcattga gtaacgtact gaccacgtca
    41821 aagtggctgg cggtgctgga atgcacaaaa accgcatcgc aactggcggc aaggctggat
    41881 aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta
    41941 ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    42001 ttttgcgcaa tgccacctaa tcccactacg ccgatacgta attttttcac tttaatctcc
    42061 cagatgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    42121 ttcgacgcgg gcctgtaaat caccatcaac cgcgttcgac atatccgtca ccgccggctg
    42181 atcttcaacc tcaccactga aaagatgcat gtagcggctt tcgcgtttac ccggttcgcg
    42241 ggccagacgc accacaaaag gaccatcttc gcgatttgcc agttgttcca gcgtcgactc
    42301 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacctgg
    42361 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttccgctg cgctcagttt
    42421 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    42481 accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    42541 ggattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac
    42601 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc
    42661 aatcacgcga gcttcaaggg cagtaagttg atatttcatg agattctcct tagcggccgg
    42721 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    42781 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    42841 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    42901 tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc
    42961 caatcgaata accgagatag caggcatgaa aagagccacg aacaacattg gaaaaattgg
    43021 caacgccaat aatctctttt tcgtccgggt cgaataagcc aaagtagaaa gctgaacctt
    43081 gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga taacagtggc
    43141 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    43201 caagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    43261 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    43321 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa
    43381 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    43441 taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    43501 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg
    43561 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    43621 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    43681 ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    43741 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg
    43801 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    43861 tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    43921 aacaacgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    43981 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    44041 ccgttttgtt tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca tggaaactct
    44101 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg
    44161 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    44221 tgccaattat ggtcaacgac gtggctggcg caccctctgc cgttaaatgg atgtatgcca
    44281 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    44341 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    44401 tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    44461 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    44521 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata
    44581 tcggtggtgg ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga
    44641 tgatgctggg cattattggc atcttcactt tccttgcgct gggttggcag tttagccaga
    44701 aacgcgccgc gcgtcgtttg cttgaacgcg acgcctgatt tgatactccc ccctgactcc
    44761 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    44821 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    44881 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    44941 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gacatcgttc
    45001 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    45061 aagagtggct gcgcaaggtc aatggctacg caaacaaact gctgttgccg cgttttgcca
    45121 aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    45181 aggccagcgc gggtaattta gccgacctgc tggcccactc tgacggtctg attaagaata
    45241 tcagcgatga tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    45301 aactttcgga agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg
    45361 gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    45421 tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg
    45481 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    45541 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    45601 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    45661 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc
    45721 ggtttacccg gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt
    45781 cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg
    45841 ttcggtagtc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    45901 gaaaaaggtg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa
    45961 aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac
    46021 tttaaccagc aacctgctta cgtcctgcgc gaagatggca gtcagggcga agcaatggcg
    46081 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat
    46141 taatgacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    46201 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    46261 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    46321 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat tgtaatgccc
    46381 aatctggctc cgcccgtgac cactgttgag gctgccgtgg cgtatcgcca gcgtattctt
    46441 gatgccgtac ctgccgggca caatttcacc ccattgatga cctgttattt aacagattcg
    46501 ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt
    46561 tatccggcaa acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg
    46621 ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    46681 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct
    46741 ctgcgccagc gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct
    46801 gccgactatg tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg
    46861 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc
    46921 atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat
    46981 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc
    47041 ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa
    47101 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat
    47161 ggcttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa
    47221 agcatcgcac tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg
    47281 tccgttaaac aataaaaatc gtagcttcct gttgtcatta ggttatttta cctgtataaa
    47341 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    47401 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    47461 tgcgtttcct gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc
    47521 ggttattggc gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    47581 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg ttt
//
LOCUS       DAZKGS010000017        45810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKGS010000017 DAZKGS010000000
VERSION     DAZKGS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            172..798
                     /locus_tag="ACHOQY_001017"
     CDS             172..798
                     /locus_tag="ACHOQY_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9677163.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            819..2921
                     /locus_tag="ACHOQY_001018"
     CDS             819..2921
                     /locus_tag="ACHOQY_001018"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9677164.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2934..3572
                     /locus_tag="ACHOQY_001019"
     CDS             2934..3572
                     /locus_tag="ACHOQY_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9677165.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3636..4304
                     /locus_tag="ACHOQY_001020"
     CDS             3636..4304
                     /locus_tag="ACHOQY_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9677166.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4301..5086
                     /gene="phsC"
                     /locus_tag="ACHOQY_001021"
     CDS             4301..5086
                     /gene="phsC"
                     /locus_tag="ACHOQY_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9677167.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5090..5902
                     /gene="ydhT"
                     /locus_tag="ACHOQY_001022"
     CDS             5090..5902
                     /gene="ydhT"
                     /locus_tag="ACHOQY_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9677168.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5914..7519)
                     /locus_tag="ACHOQY_001023"
                     /pseudo
     CDS             complement(5914..7519)
                     /locus_tag="ACHOQY_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7645..7950)
                     /locus_tag="ACHOQY_001024"
     CDS             complement(7645..7950)
                     /locus_tag="ACHOQY_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9677169.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8058..8134)
                     /locus_tag="ACHOQY_001025"
     tRNA            complement(8058..8134)
                     /locus_tag="ACHOQY_001025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8098..8100),aa:Val,seq:gac)
     gene            complement(8139..8215)
                     /locus_tag="ACHOQY_001026"
     tRNA            complement(8139..8215)
                     /locus_tag="ACHOQY_001026"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8179..8181),aa:Val,seq:gac)
     gene            8523..9779
                     /locus_tag="ACHOQY_001027"
     CDS             8523..9779
                     /locus_tag="ACHOQY_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9677170.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9820..11193)
                     /gene="mdtK"
                     /locus_tag="ACHOQY_001028"
     CDS             complement(9820..11193)
                     /gene="mdtK"
                     /locus_tag="ACHOQY_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9677171.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11408..12049
                     /gene="ribE"
                     /locus_tag="ACHOQY_001029"
     CDS             11408..12049
                     /gene="ribE"
                     /locus_tag="ACHOQY_001029"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9677172.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12089..13237)
                     /gene="cfa"
                     /locus_tag="ACHOQY_001030"
     CDS             complement(12089..13237)
                     /gene="cfa"
                     /locus_tag="ACHOQY_001030"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9677173.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13528..14739)
                     /gene="punC"
                     /locus_tag="ACHOQY_001031"
     CDS             complement(13528..14739)
                     /gene="punC"
                     /locus_tag="ACHOQY_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9677174.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14852..15784
                     /gene="punR"
                     /locus_tag="ACHOQY_001032"
     CDS             14852..15784
                     /gene="punR"
                     /locus_tag="ACHOQY_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9677175.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15781..16806)
                     /gene="purR"
                     /locus_tag="ACHOQY_001033"
     CDS             complement(15781..16806)
                     /gene="purR"
                     /locus_tag="ACHOQY_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9677176.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17105..17194
                     /gene="cydH"
                     /locus_tag="ACHOQY_001034"
                     /gene_synonym="ynhF"
     CDS             17105..17194
                     /gene="cydH"
                     /locus_tag="ACHOQY_001034"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9677177.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17360..18529
                     /locus_tag="ACHOQY_001035"
     CDS             17360..18529
                     /locus_tag="ACHOQY_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677178.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18675..19256)
                     /gene="sodB"
                     /locus_tag="ACHOQY_001036"
     CDS             complement(18675..19256)
                     /gene="sodB"
                     /locus_tag="ACHOQY_001036"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9677179.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19384..20211)
                     /gene="mepH"
                     /locus_tag="ACHOQY_001037"
     CDS             complement(19384..20211)
                     /gene="mepH"
                     /locus_tag="ACHOQY_001037"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9677180.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20546..20893
                     /gene="grxD"
                     /locus_tag="ACHOQY_001038"
     CDS             20546..20893
                     /gene="grxD"
                     /locus_tag="ACHOQY_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9677181.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20944..25560)
                     /locus_tag="ACHOQY_001039"
     CDS             complement(20944..25560)
                     /locus_tag="ACHOQY_001039"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP9677182.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPDGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25653..26300)
                     /gene="rnt"
                     /locus_tag="ACHOQY_001040"
     CDS             complement(25653..26300)
                     /gene="rnt"
                     /locus_tag="ACHOQY_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9677183.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26403..26810)
                     /gene="gloA"
                     /locus_tag="ACHOQY_001041"
     CDS             complement(26403..26810)
                     /gene="gloA"
                     /locus_tag="ACHOQY_001041"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9677184.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26891..27988)
                     /gene="nemA"
                     /locus_tag="ACHOQY_001042"
     CDS             complement(26891..27988)
                     /gene="nemA"
                     /locus_tag="ACHOQY_001042"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9677185.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28025..28624)
                     /gene="nemR"
                     /locus_tag="ACHOQY_001043"
     CDS             complement(28025..28624)
                     /gene="nemR"
                     /locus_tag="ACHOQY_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9677186.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28727..28966
                     /locus_tag="ACHOQY_001044"
     CDS             28727..28966
                     /locus_tag="ACHOQY_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9677187.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29015..29911
                     /locus_tag="ACHOQY_001045"
     CDS             29015..29911
                     /locus_tag="ACHOQY_001045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9677188.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29992..30513
                     /gene="sodC"
                     /locus_tag="ACHOQY_001046"
     CDS             29992..30513
                     /gene="sodC"
                     /locus_tag="ACHOQY_001046"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9677189.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30514..32526)
                     /locus_tag="ACHOQY_001047"
     CDS             complement(30514..32526)
                     /locus_tag="ACHOQY_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9677190.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32526..33383)
                     /locus_tag="ACHOQY_001048"
     CDS             complement(32526..33383)
                     /locus_tag="ACHOQY_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9677191.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33386..33622)
                     /locus_tag="ACHOQY_001049"
     CDS             complement(33386..33622)
                     /locus_tag="ACHOQY_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9677192.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33823..34257
                     /gene="slyA"
                     /locus_tag="ACHOQY_001050"
     CDS             33823..34257
                     /gene="slyA"
                     /locus_tag="ACHOQY_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9677193.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34304..34771)
                     /gene="slyB"
                     /locus_tag="ACHOQY_001051"
     CDS             complement(34304..34771)
                     /gene="slyB"
                     /locus_tag="ACHOQY_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9677194.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35045..36154
                     /gene="anmK"
                     /locus_tag="ACHOQY_001052"
     CDS             35045..36154
                     /gene="anmK"
                     /locus_tag="ACHOQY_001052"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9677195.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36252..36581
                     /gene="mliC"
                     /locus_tag="ACHOQY_001053"
     CDS             36252..36581
                     /gene="mliC"
                     /locus_tag="ACHOQY_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9677196.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36640..37296
                     /gene="pdxH"
                     /locus_tag="ACHOQY_001054"
     CDS             36640..37296
                     /gene="pdxH"
                     /locus_tag="ACHOQY_001054"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9677197.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37425..38699
                     /gene="tyrS"
                     /locus_tag="ACHOQY_001055"
     CDS             37425..38699
                     /gene="tyrS"
                     /locus_tag="ACHOQY_001055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9677198.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38758..39621
                     /gene="pdxY"
                     /locus_tag="ACHOQY_001056"
     CDS             38758..39621
                     /gene="pdxY"
                     /locus_tag="ACHOQY_001056"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP9677199.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39665..40270)
                     /gene="gstA"
                     /locus_tag="ACHOQY_001057"
     CDS             complement(39665..40270)
                     /gene="gstA"
                     /locus_tag="ACHOQY_001057"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9677200.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40376..41878)
                     /gene="dtpA"
                     /locus_tag="ACHOQY_001058"
     CDS             complement(40376..41878)
                     /gene="dtpA"
                     /locus_tag="ACHOQY_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9677201.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42489..43124)
                     /gene="nth"
                     /locus_tag="ACHOQY_001059"
     CDS             complement(42489..43124)
                     /gene="nth"
                     /locus_tag="ACHOQY_001059"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9677202.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43124..43819)
                     /gene="rsxE"
                     /locus_tag="ACHOQY_001060"
     CDS             complement(43124..43819)
                     /gene="rsxE"
                     /locus_tag="ACHOQY_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9677203.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43823..44443)
                     /gene="rsxG"
                     /locus_tag="ACHOQY_001061"
     CDS             complement(43823..44443)
                     /gene="rsxG"
                     /locus_tag="ACHOQY_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9677204.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44447..45505)
                     /gene="rsxD"
                     /locus_tag="ACHOQY_001062"
     CDS             complement(44447..45505)
                     /gene="rsxD"
                     /locus_tag="ACHOQY_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9677205.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45506..>45810)
                     /locus_tag="ACHOQY_001063"
     CDS             complement(45506..>45810)
                     /locus_tag="ACHOQY_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677206.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT        11522 a        11333 c        11951 g        11004 t
ORIGIN      
        1 tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat acactatcgg
       61 gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg aatgcttaat
      121 atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg
      181 gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg tatatcagga
      241 aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg ttgcaaacag
      301 gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt gctggtgacc
      361 cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat
      421 ggctcagtag ggacattctt ctcccgtatc aaaatccacc gcaattattt ctttggcgac
      481 aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc ggacacctgc
      541 cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat tacctggcag
      601 cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag cgcctgtact
      661 acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt
      721 gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat tatcgagtgg
      781 aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt tggacaggta
      841 atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc agtaagttta
      901 aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag
      961 gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca ttaactggat
     1021 ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt accaaaggaa
     1081 atttagttgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa ttcgctggat
     1141 acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag attaaagatg
     1201 acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc gcaacgacgg
     1261 aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg
     1321 aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc ggtgcgggaa
     1381 ctggcgcaat tatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg
     1441 tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg actgaactta
     1501 ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca gagtattcag
     1561 atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct gaaggtggtc
     1621 cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt cgtacctata
     1681 aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg agtggctgcc
     1741 actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg
     1801 tgcccagcac aggtggtaac acttgtgtag ctaactttgt ccataccacc atcttcccga
     1861 acgggccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac ctggttggtc
     1921 tgaatttgtt cgatgactac ggcctatggt gtaactacgg gcagctgcat cgcgacttta
     1981 cttattgtta cagcaaaggg gtgttcaagc gtgttctgcc agctgaagag tatgcggaaa
     2041 ttcactggga tcaactggaa gcgggtgacg ttaatttcat taaagatttt tactaccgtc
     2101 tggcgcatcg tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc atcgcagaac
     2161 gctggaattt gggtgaagag tactggggct acgcaaaaaa taaactctgg tcgccgtttg
     2221 gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt gttaactgta
     2281 tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcg ggtttgccat
     2341 tgaaactgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgcc tacgatgaaa
     2401 ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgcaaagtgg tcgctgttgc
     2461 gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg accgtttcgc
     2521 cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc ttcaaagcga
     2581 tcaccggcga agagatgact caggaaaaat tagatttagc tgcagagcgt atctttacgt
     2641 tgcatcgtgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat gaacacgatc
     2701 ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact gaaggtactg
     2761 acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa gaaatgggct
     2821 gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg ctggaagata
     2881 ttgccgccga tctggcagca cacaatctac tgcctgtgta aggaatgggt gagatgaacc
     2941 atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag cggcaatggt
     3001 tacaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct gaaatcctgg
     3061 cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc gtgtttctct
     3121 cgccacccta ttcggttgca gaagaggagc tgtcagtttt gctggaaagc attaagcaga
     3181 acggagacta cgcggatatc gcttgtatga cgggaagcca ggatgattac tattactcaa
     3241 cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag caggacattt
     3301 gtcgcgccat tgcacacgcc gttcgttttg agtgccagac atatcctcgt ccttacaaag
     3361 tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa gccgcgatcg
     3421 ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca tcgacggcgg
     3481 tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc ttatgcgaat
     3541 ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca tggatagggt
     3601 ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga cgcaaatttg
     3661 ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg ttagcgaaca
     3721 cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg tgcaatggct
     3781 gtaatatttg cgcccgcgca tgccgtaaaa ctaaccacgt ccccgctcag ggaagccgtt
     3841 tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat cacttttttc
     3901 gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg acaggggcgt
     3961 cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc ggctgcagtt
     4021 attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact aaagtggcag
     4081 ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc atttgcgtca
     4141 gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa attcaggcgt
     4201 ggttgcagca aaataaatac tatcaatatc agctacctgg tgccgggaaa ccgcatctgt
     4261 atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt cgcaacatgc
     4321 tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg aattttggcc
     4381 ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc tggggctgca
     4441 tgccttgctt cgtgctcgtg gcgtgaagaa atcagtcacc gattatggtg agaagattta
     4501 tctttactgc aaagcggtca gattatggca ctggtcgaat gcgttactct ttgtattgtt
     4561 gctggcaagt gggctgataa atcactttgc gctggtgggc gcaactgcgg ttaaaagtct
     4621 ggttgcggtg catgaagttt gcggattttt gttactcgca tgctggctcg gctttgtgct
     4681 gatcaatgcc gttgggggta atggtcacca ctatcgcatt cgtcgtcagg ggtggctgga
     4741 gcgagcggca aaacaaacgc gattttattt gtttggcatt atgcaggggg aagaacatcc
     4801 tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct atgttggtgt
     4861 tatgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct atccgcaagc
     4921 cgtgggagat gtgtttcctg gcgtaagata ctggttattg caggcgcatt ttgctctggc
     4981 atttataagc ctctttttta tcttcggtca tctttatctt tgcaccacgg ggcgtacgcc
     5041 acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa tgatcattac
     5101 acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt tcctgcgcca
     5161 tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg aaggatatac
     5221 ggactgggcg gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg atgaaaactt
     5281 cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gaccccggag agataccatt
     5341 ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg aggataacgc
     5401 ccgaatcgca accgctgggt atgccgcgca aattgccagt atgggttata gcgtgcggat
     5461 tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac gggttgctgt
     5521 aaccggtaat tcttcgcgga taagcagcgc tggagatagt agtcggattg cgaataccgg
     5581 aatgcgagta cgcgtttgta cattgggtga acggtgtcat gtcgccagta atggtgacct
     5641 ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg acaatgtaca
     5701 catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg actcaattat
     5761 tctcgggcca gggggaagcg ccgcactggc atatcatgac ggcgaaagag ttcgcttcgc
     5821 cgtcgccatt gaaggtgaaa acaatattcg aaccggagtc cgctatcgtc ttaatgaaca
     5881 gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac gccgacgagc
     5941 acgggatgca ggcttcacga ccgcccgagc catcgcctca ccaatttctg cacatgccgt
     6001 aagtccctga acgaaaggct ggtcgtgcat caaccagggc aacgcgccga acgctacgcg
     6061 tccacgaata tcttcgggtt gctgtaacgt ataatcttcg ccaatatcag ggatttcatc
     6121 ccctgttttc tgtaattgct cgcgtagccc agggaaagga atatctttca ctttaagcgg
     6181 acgctgtccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt ccgttttcag
     6241 gacggtgcgc gactcattaa tttccatttt gtagtcttca ccgagggcga gaatatgaat
     6301 gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga ttgccgcata
     6361 attatcgatg aaaaccctgg caaggccttt actgaaccgt ttatggtcgt gttcattcag
     6421 atgaggaaca atttcctgta cggcttcatg caggcgcaga atgacataac gccagggaac
     6481 agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg cccagtcaaa
     6541 tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga cattcaggct
     6601 ctctaaggct atgcgttggc tccagtctgg atcagcaaac ttgatttcct ctactatcaa
     6661 tctaaatacc cgatccaaaa ggccctcttc acctttttga atctcagcat ttaatgcctg
     6721 atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg cttcgggtaa
     6781 aatacccgtg cgcgacatca acgtaatatt tagcttttca cttgcgttat cgcggttaaa
     6841 aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca ctgccattgc
     6901 cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat cgacctttgc
     6961 ttccatcagg cctgaccacg gactgggaaa atacgttctg gttgcttctt cttcatcagg
     7021 ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta aatcctgatt
     7081 tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacctggc atgattcata
     7141 aacagccact gcaaattttt gctgtcgtgc ctggtctact aatcgtaaaa attgatcgcg
     7201 gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg tttctttttt
     7261 taacgccata acgctggaga tggctggctt cttgcttttg tagccattcg agatacgtac
     7321 aattaatcgg cggtatttca atactggcaa tatttgccag catcattttt gagttttcct
     7381 catcactgta tggcatcccg acaccggcct cgtcagcctg ctcgaagata gaaatagaaa
     7441 gtggagtttg ttgctgtaga agcgagaata aggtgtagat ccccgtaggc ccggcaccca
     7501 caatagcaat ttttttcatt gttacttacc tgtacgcatt ctttcgaatt tagtgttatc
     7561 agataagttt ggttgagatt tttagaattt gagaaatttc agacaggtat gaaaagggcg
     7621 ctaaccgccc ttgattaaat tctgtcaggc gagttttgcc tgattgattt gactcagtgg
     7681 ctcattgaca tcgaaaactt tggcgacaac ttcctcaacg ccgagatttt taagtcgggc
     7741 agtatgtttt tccagatagg caagggcgct tttttcatca gtaaaaagat agatcccacc
     7801 ggcttcgtgg ttcttttcac tttctgtcca taccttccac agaaaaccag gttcctgatt
     7861 aatcgactca gcaagtggtt taagctgctc agccattgcg tcgccaaatg ggccattaaa
     7921 agcaaaatga agttgtaaca aagttgccat agcaattctc cttctgataa cgcatacgaa
     7981 gacagtgtaa atcttttaac gtattggata cagagaagaa acagaggaaa aaaggagaag
     8041 accagattaa gaaaatctgg tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc
     8101 aaggtggtgc tctaaccaac tgagctacgg acgcaggatg gtgcgttcaa ttggactcga
     8161 accaacgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt
     8221 tgtaggtgac aacggggacg aatattagcg gcagagtggg aaggtggcaa gaggcaaaac
     8281 gtaattttct gcgctatttc gaccgtttgc agagctttta agcaaattgg ctatattttg
     8341 ttgatttgca agggcgattt ttattcagga tcgcatttac atctgataca acccaataat
     8401 tcgtgtatca gagtttaaac agagttgttt gccaccatct caaatctgtc gtggataaat
     8461 cctccgtagc ctttacactt aggtagcaat aaaggcacac agatgcagca aaggaataaa
     8521 tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa tgtgcaaatt gttaagaccg
     8581 gcgaggtcat tggcgcgacg caacttactg aaggcgagtt gattgttgaa gctggcggaa
     8641 gagccgaaaa taccgtggtc acgggggctg gctggttgaa agtggcaacc ggtgggatcg
     8701 ccaaatgcac acagtacggt aacaatggca cgctatcggt cagcgacggt gccattgcca
     8761 cagatattgt tcagtccgag ggaggcgcaa ttagtctctc tacgctcgct acggttaatg
     8821 gccgccatcc cgaaggtgaa ttcagcgttg atcagggtta tgcctgcggt ttgttgctgg
     8881 aaaatggcgg taacctgcgt gtactggaag gacatcgcgc ggaaaaaatc attctcgatc
     8941 aagagggcgg cctgttggtc aatgggacaa cctcagctgt cgtggtagat gaaggtggtg
     9001 aattgttggt gtatccaggt ggggaagcca gcaattgtga aattaatcag ggcggcgttt
     9061 ttatgctggc cgggaaagcc agtgatacgt tgcttgctgg tggcaccatg aataatctcg
     9121 gtggtgaaga ctctgacact attgttgaga atggatccat ttatcgtctg gggacggatg
     9181 gccttcagct ctacagttcc ggtaagacgc aaaacctgtc cgtgaatgtg ggtggtcggg
     9241 ctgaagtgca tgccggtacg ctggaaaatg cggtaataca aggtggaaca gtgatcctgt
     9301 tgtcacccac cagcgcggac gaaaattttg tcgtagagga agatcgcgca ccggttgaac
     9361 tgaccgggag tgttgcatta ctggacggcg cttcaatgat tattggctat ggtgcagatc
     9421 tgcaacaatc aacgattact gtacagcagg gcggtgtgtt gattctcgac ggcagtacgg
     9481 taaaaggtga cagtgtcact tttagtgttg gtaacatcaa tctgaatggc ggaaaactgt
     9541 ggctgatcac tggtgcggca acgcatgtgc aactgaaagt gaaacgcctg cgcggagagg
     9601 gagcgatttg cctgcaaacc agtgcgaaag aaatctcacc tgacttcatc aatgtgaaag
     9661 gggaagttac cggggatata cgcgttgaga taacagatgc cagtcggcaa actctgtgta
     9721 acgcactgaa actacagcca gacgaagacg ggattggcgc aacgctccag cctgcgtaaa
     9781 aacacaggcg caacccgtga aggttgcgcc ttgtctttat tagcgggacg ctcgttgcag
     9841 aatgatggct gacggcagac gttgcaggaa ccgcatacgc agcatcatca taatggctgc
     9901 cgacgtcagg ccaataataa agcctatcca gaagcctgct ggccccatag gttcaacgac
     9961 cagatcggtc agtgccagaa tatagccgct tggcaagccc agcacccagt aagccgtaaa
    10021 ggtaatatag aaaatggaac gcgtatcttt ataaccacgc aaaatcccac tgccaatcac
    10081 ctggatcgag tcagaaatct gatataccgc cgccagcaac atcaaatgcg cagccagcgt
    10141 tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa
    10201 tatggccgtc agggttgcca tacagacacc caccataagc ccggtccgcg cagcggtttg
    10261 cgcatccagc gttgagccct gacccagacg ataacctacg cggatagtta ccgctgccgc
    10321 cagcgacatg ggaagcacga acattagtga actaaagttc agggcaatct ggtgtcctgc
    10381 gacatcaaca ataccgagcg gagacactaa cagagccacg acagcaaaca gtgtcacttc
    10441 aaagaacagt gccagcgcaa tcggcaaacc gagttgaatc agtcgtttca taaccgcagg
    10501 atcgggtttc gcggtgcctt tttcgttacg aatatcgcgc atggagcggg cgcgtttaat
    10561 gtaagaaacc atggcaagga acataaccca gtacaccgcc gcagtagcca cgccacaacc
    10621 aacgccaccg agctcaggca taccgaaatg accataaata aagatatagt tcaccggaat
    10681 gttcaccagc aggccgataa agcccattac catacccggc ttggtttttg ccagaccttc
    10741 acactggtta cgggcaacct ggaagaacag atagcccggc gcgccccaca acaacgcacg
    10801 cagataaccc acggctttgt ccgccagagc cggatcgatg ttttccatgg agcggataat
    10861 gtaacctgca ttccacagca ccagcataat gagaacggaa acaaaacctg ccagccagaa
    10921 accttgtcgc acctgatgcg caatgcgctc acgtcgaccg gaaccattta attgcgcgat
    10981 aaccggcgtt aatgccagca gcagtccgtg accaaagagg atcgccggaa gccagataga
    11041 agtaccgata gcgaccgccg ccatgtcggt ggcactatag ccgcccgcca tcacggtatc
    11101 gacaaaaccc atcgcagttt gggcgatttg cgcgagaatc accgggattg ctaatgctaa
    11161 taacagacgc gcttcactga tatacttctg cacgtgaaca ccttttattt gtagttatat
    11221 gagagactaa aaagccgcct gaatgggcag caaaaagaag aagcagggga aattcagtct
    11281 attgtagcga ggatttatta attctccagt gaaaaaatag tcattcttac cccttcactg
    11341 gtaagggcgt ttgccacctg ctattgtgct gaacagaatg tcttaactga tttcaggagt
    11401 tgtaagtatg tttacgggga ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa
    11461 accaaatttt cgtacgcatg tggtggagtt acccgaccac atgctggacg gcctggaaac
    11521 cggtgcgtcc gtggcgcata acggttgctg cctgaccgtg acggaaatta acggcaacca
    11581 tgtcagtttt gacctgatga aagaaacgtt acgcattacc aatcttggcg atttaaaagt
    11641 gggggattgg gtaaacgttg agcgtgcggc gaaattcagt gatgaaattg gcggacactt
    11701 aatgtcaggt catattatga ccactgctga agtggcgaaa atattaacct cagaaaataa
    11761 tcgccagatc tggtttaaag tccaggatag tcagttgatg aaatatattc tgtacaaagg
    11821 atttattggc atcgacggta ttagcctgac cgtcggcgaa gtcacgccaa cgcgtttttg
    11881 cgtccattta attccggaaa cactggaacg cacgactctt gggaagaaaa aactcggcgc
    11941 acgcgtcaac attgaaattg atccacaaac tcaggcagtg gtagatacgg tagaacgtgt
    12001 gctggcggca cgagaaaatg ccatgaatca accaggtaca gaagcctgat ctgaactcgc
    12061 ccccgaagag ggggcgatag aatagccttt agcgagcgac tcgaaggccg ttttcaacac
    12121 cgcgtgagaa caccacctgc cagagctgaa tatcacgggc gcggaaagca cctgcacaag
    12181 cattcagata ataggtaaac attcgtttaa agcgttcact gtagttatct gcaatttctg
    12241 gccatgcggc gaggaatcgt tcataccacg ccatcaacgt agtatcgtaa tcagcaccga
    12301 agttatgcca gtcttccatc acaaagtggg gttcgctgga ctgagcaatc tggcgtacag
    12361 agggcaggca accgttcgga aaaatatatt tattaatcca gggatcaaca ttcagatcgg
    12421 tttttttcga accgatagta tggagcagga atataccttc cggtttcaaa ttacgatcca
    12481 ccaccgcaaa ataggtatcg taatttttcg gtccgacgtg ctcgaacatc cccacagaaa
    12541 caatacgatc aaactggtcg ttcaggtcac gataatcttg cagcaaaatg gtgacatcca
    12601 ggccttcaca gcgttcctga gccatttttt gctgttcggc agaaatggtg acgccgacca
    12661 cgcttacgtc atagttagat gccatgtagt gcgccagtcc gccccagccg cagccaatat
    12721 ccagtacgcg catccctggt tttaactgca atttttcaca aatcattttg agcttcgcct
    12781 gctgggcaga ttccagatta tcggcatctt tccagtaagc gcaggaatat tgcatgaagg
    12841 gatcaagcat gcgactgaac aagtcattac ccaaatcgta atgctctttg ccgactatcc
    12901 aggcacgttt tttactctgc agattgaaga gacgggcgcc tgcaatacgc agcgtgtctt
    12961 tgaaatgatg ggggagttgg ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat
    13021 ccagtcggtc acattcccac cagccatcca tataactttc gcctaacccc aaagagcctt
    13081 cttgcagaac gcgtttaaaa aaatcggggt ttttcacacg aatatccgcc ggggcagaac
    13141 cgttaatggc tataccggca cggctaagta attcgttggc gatacggtac cagttgtcat
    13201 ccggtacact gacttcttct atacacgatg aactcatagt ttctccatca ctggtcggtg
    13261 atcagaacct taaaacagcg tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa
    13321 accgcgagtc tataatccga cgtagaacag agggaaagga ggaacccttg ccgaaatgac
    13381 catccatagt gagtcgggaa cgatcctgtc cccgtaaaaa cataatattt atcgtagctt
    13441 taacaactcg aaattagtat aggccgctaa tttcaatcat acaacaaaat tttgttagca
    13501 gcctaagtat aagtatatcg atatagatca gtgtgattcg ctatgagcga cttcggcatt
    13561 gccatgattc tggcagccaa cttcttcaca acgttgcatc atgtaaccca gcgcgaccag
    13621 cactactgtt gataacatca cgctggtggt ggtgagcaat ggcgtgctga tactgatcag
    13681 ccaggaaact accagacttg cgaggaagca cagacccagt tgcagagtgt tctgcaacgc
    13741 tgcggcgcga ccggttgcat gtgggaaggg acgcagcgcc tgggcgacaa caatagggta
    13801 gatcgcgcca ttggcaatcg ccatcacaca gaatgggatc aggatttcga ccagcgacac
    13861 atggctaatg aagcccgcag cccaggtcgc aatgacgctg acagcaaaca gcaccagcaa
    13921 ccacggtaat aactgcttgc cttgccattt ctgcagtgcg gcgcgacagc cataaccacc
    13981 aatcagaaac gcgatagttt gcgggacata acttaaacca ataactgccg ggctgtagcc
    14041 catttcacta aggatgaacg gtgaaccggt cagccatgca aaaaaactgg ctgaacaggc
    14101 tgcatatatc agcacgttgc cgcgataggt tttagaacgt agcaggtcgg taaaggtcag
    14161 accatcctga ctattgttac gggccttcgt cgtgggtttg agccagaaaa taggcagaat
    14221 cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc tgccaggaaa aatggaccag
    14281 cagccagctt cctaacaggg gagccagtgc cggagacaga cccaccagcg gcatgatggt
    14341 cgcaaaaata cggttgactt tctgtgaagg ataataatct gtcactaatg cttgccagat
    14401 aaccgccgcg gcgcagaccc ccacagcctg tacaaaacgc aataccagca gcgtagcggc
    14461 gttttctacc cacagcatcc ccagactacc taacgcaaaa attgtcaggc cgattaataa
    14521 caccggttta cgaccataac ggtcggagag tggcccccac agaagctggg ctgcggcaaa
    14581 accggcaagg aacagactaa ggctggcact gacagcagac gcaggcgttt gcaggtcggc
    14641 ctgtatggcg gcgaaagcag gcagatacat atcggttgcc agaaaaccga gtacgctcaa
    14701 acccgccagc cagactaaaa atcttttccc aggttgcatg tatattctct aaatctctca
    14761 attaaacacc gccgcaaagt gtagggagtg aaattagtgt tgtgaaacgg taatatttga
    14821 tgactggttt caaaaatttt gcaggcagac tatgtggtca gaatattcac tcgaagttgt
    14881 tgatgcggta gcgcgtaatg gtagttttag cgctgcggca caggagctgc atcgcgttcc
    14941 ttctgcggtc agctataccg tgcgtcagct ggaagagtgg ctggcggtac cgctctttga
    15001 acgacggcac cgtgatgtgg agctgaccgc tgctggagcg tggtttctca aagaagggcg
    15061 ctctgttgtc aaaaaaatgc agatcacccg ccagcaatgt cagcagatag cgaacggctg
    15121 gcgcggtcag ttagctatcg cagtggataa tattgtcagg ccagaacgta cacggcagat
    15181 gatcgttgat ttttatcgcc attttgacga cgtcgaactt ctggtctttc aggaagtgtt
    15241 taacggtgtc tgggatgcgc tttccgatgg gcgcgtggaa ctggcgattg gcgcaacacg
    15301 cgcgattccg gtaggcggtc gttatgcctt ccgggatatg gggatgctaa gctggagttg
    15361 cgttgttgcc agccaccacc cgctggcgtt gatggatggc ccgttcagcg atgatacgtt
    15421 gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga acgctaccta aacgtattac
    15481 ctggttgctg gataaccaaa aaagagttgt cgtgcctgac tgggaatcat cggcaacctg
    15541 tatctcggca ggattatgca tagggatggt gccaacacat ttcgccaaac cgtggcttaa
    15601 tgaggggaag tgggtggcat tagaactgga aaaccccttt ccggattcgg catgttgtct
    15661 gacatggcag caaaatgata tgtcgcctgc gttaacctgg ctgcttgagt atctcggcga
    15721 tagtgagacg ctgaataagg agtggctgcg ggagccggaa gagactcccg caacgggtga
    15781 ttaacgacga tagtcgcgga acgggccgtc agccacggag cggcgttcaa tcaagcgcgg
    15841 atgcacttca atggactgcg gttcttcacg tttgttgacg atacgatcca acagcatgtt
    15901 gaacgctgtt tcacccagcg aatcttttgg ctggtggatc gtggtcagcg ccggcgtaaa
    15961 atagcgggcg ttgcgcacgt tatcataacc gatcagcgaa acatcctgcg ggacgcgcag
    16021 acccatttca tcagcagcac aaagtgcgcc cattgccatg atatcgccac cacagaagac
    16081 ggcagtaggg cgatgcggct gcgacagtat ttgctgcatg gcgcgataac cagattcggg
    16141 ttcaaagtca ccctgaacaa tccagctttc cggcaccttg atcatcgctt cttccatcgc
    16201 cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt tccagcgggc cggggatgac
    16261 gccgatttcg cggtgaccgc gttcaatcag ataacgcccg gccatgtagc cgccttcgaa
    16321 cgcgttatca atgaccgcat cggtgaagtc agcttttgct tcaccccagt ccatcaccac
    16381 cattgggata tggcgatact cttccagcat cgccagcaac ggctctgggt actcagaaca
    16441 catcaccagc agaccatcga cgcgtttttg cgccatcatc gacagataag cccgctgttt
    16501 ctcaagattg ttccacgcat tacccagaat cagggtgtaa cctttctgga agcaattttt
    16561 ttcaaccgct tcaatgatct cggcaaaata ggccgcttcg ctgctggtcg ccagcaaacc
    16621 gatagacttg gtgtggttaa ccttcaggct acgcgccacc gcgctagggg agtagtgtaa
    16681 ttctttaatc gctgcccaca cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt
    16741 gattacgtgt gacacagttg tagtggaaac gtttgctcgt ttcgctacat cttttattgt
    16801 tgccattcca tttcactcca gaccctaact tcagctcctg caaaatcgca aggtaaacgt
    16861 ttgcctttac acaccttacg cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg
    16921 agcgtcagga aggggtcaat ggccagtacg caaatagtgt aagcgtggaa ttttggccga
    16981 tcttgacgaa aaggggaagt ggtaaaaaca cttatcatta taaatgacgg gagatttttt
    17041 catcacagtg tgtaaaaatg cgtaatatca ctatttgtgt ggtaataaaa aaggagaaaa
    17101 cttgatgagc accgacctta aattttcact ggtaacaacg attatcgtcc tcggtttgat
    17161 cgtggccgtg ggtttgactg ccgcgctgca ctgatttcct ctcgagggag cgcgttctcc
    17221 ctcgcatctc cttcctgttt gttagcaaaa tggcaaaagt gttttctctt tttgttattt
    17281 tcattttttt gtgattgctg tcatgctttc gccactttaa tgaatatcgc gtaatgacgc
    17341 gaagaggtgg agataaggca tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc
    17401 gtttggtatc gggacaacgg agttctctcc aatgggtttg ttgcccgtca ttgcgcgcgg
    17461 tgtggatgtc tcgattcccg ctgccggaat gttaatcagt gcctatgcag ttggcgtaat
    17521 ggttggcgcg ccgctgatga cgcttctact ttctcatcgt gcccgccgca gtgcgttgat
    17581 tttcctgatg gcaattttca cgctcggcaa cgtactttcc gccatcgcgc cggattatat
    17641 gaccctgatg ctttcacgca ttttgaccag cctgaatcac ggagcatttt ttggtttggg
    17701 ttcagtcgtg gccgcaagcg tggtgccaaa acataaacag gccagcgcag ttgccactat
    17761 gtttatgggg ttaaccctgg caaatatcgg tggcgtgccg gcggcgacct ggttgggtga
    17821 aaccatcggc tggcggatgt catttctggc aacggcgggg ctgggagtga tttcaatggt
    17881 aagtctgttc ttctcattac ctaaaggtgg tgcaggggca cgacctgaag tgaaaaaaga
    17941 gctggcggta ttaatgcgtc cgcaggtgct gtctgcattg ctgacgacgg tactgggagc
    18001 tggtgcaatg tttactctct acacctatat ctctccggta ctacaaagta ttacccacgc
    18061 aacaccagtg ttcgtcacgg caatgctggt gctgattggt gtcggattct ctatcggtaa
    18121 ctatctcggc ggcaaactgg cagatcgttc agttaacggc acgttgaaag gctttttgtt
    18181 gttgttgatg gtgattatgc tggcaatccc gttcctggcc cgcaatgagt tcggcgcagc
    18241 tattagcatg gtggtgtggg gcgcagcaac ctttgcggtc gtaccgccgt tacagatgcg
    18301 cgtgatgcgt gtcgccagtg aagcgccagg tctgtcttca tcagtcaata ttggtgcctt
    18361 taatcttgga aatgcgctgg gagcagctgc tggtggtgcg gtaatttccg ctgggctggg
    18421 atacagcttt gtgccggtga tgggagcgat tgtcgcggga ctggcattat tgctggtgtt
    18481 tatgtcagcc agaaaacaac ctgaaacagt ttgcgttgct aacagctaat taaacaaaaa
    18541 cgcagaagga taaccttctg cgttacagtt gctgacaacg tgcgatttat cttgccgaat
    18601 gcgctgttaa cgcctcatcc gcgttggcat ggtaagcgaa gcgcatcagg caatgctgca
    18661 tttgccatca gttattatgc agcgagattt ttcgctacga attcccagtt caccagcgcc
    18721 cagaagtgct ccagatagcc aggacgtgca ttgcgatagt cgatgtaata agcgtgttcc
    18781 cagacatcaa cggtcagcag cggagtcgca tcggtggtca gcggagtacc cgcgttagag
    18841 gttgaaacga tagccagttt gccatcgctg tttttcacca gccaggtcca gccagaacca
    18901 aagtttttga tcgctgcatc agtaaactgc gctttgaaat cggcaaagct accaaaagat
    18961 gcggcgatag cttcagcgac ttttccagtc ggttcgccac cggcgttcgg tgccaggcag
    19021 ttccagtaga aagtatggtt ccagacctga gctgcgttgt tgaatacgcc accttcagag
    19081 ctgcgaataa tctcttccag tgatttacct tcaaacgcgg tacctttaat caggttgttc
    19141 aggttagtga cataagtctg atgatgcttg ccgtagtgat actcgatagt ttccgcagaa
    19201 atgtgcggtg ccagagcatc tttagcatat ggtagtgcag gtaattcgaa tgacattgct
    19261 actctccttt attattaatt tgcatacgta caatagcctt attgtgcgta tggatagggt
    19321 agcaaattaa aaggtgagac aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa
    19381 gttttagcga agtgtttttg gggtcattac ccgacgagcg ccaacatagt ggcgctgcca
    19441 gtagtcttca ctgagagaag tgatttgaat ttcctgacct gtgcgcggtg actgaataaa
    19501 tttgccgttg ccgacataca cgccgacatg atcggctgtg ccgcgtccct gagtacggaa
    19561 aaagaccagg tcgccgtttt tcagttcact acgttcgatt ggcgctgcat cacgcaggtg
    19621 atacatttca ttcgccgtac gcggaatacg aattttcacc aaatctttat aagcgtaata
    19681 aaccaggccg ctgcaatcaa aaccggtacg cggtgagctg ccaccccaac gatatggctt
    19741 accaatttgc tgcatcagtt tattcattgc cactttcgta gctttttgca ccttcgcttt
    19801 gtgcgcgtcg gcaagagttc ctgacgcggc atttttgact ttcacacaat gcgatttata
    19861 gcccttacgt ttggtacact tttcggtaaa gctggctgag gccgttttat tgacggcgtg
    19921 tttgctgcga gttttggcgt ttttagaagc ggtaatggaa gattttttaa cggaagaagc
    19981 ggttttgctt ttggtcgtac ttttactggc ggcttttttc gctgttttct ggctggcttt
    20041 ttgggtcttt ttggtggttg ccgtgctttt ctttttatct gcttttttgg tgatatgagt
    20101 ggtagcagaa ctctccctgg cttgctttga agcatgggcc ataggcgtta aaggcagggt
    20161 ggtaaaaagt aaagcacaga gcgtgatcga aatacggttt atccgcgcca ctgagcgtcc
    20221 ctctgttaag gcaggtctat catgcctgcg tctgatttac aaaacttgat gattctaatg
    20281 catagaagaa gagaaagtta atagctttta ttgttaaaaa atacgtttcg ttggcgagcg
    20341 caaaaaaatg cagcagtgaa agctaaaaat ggttataaat acgccagttt gccgagcatc
    20401 tgggaagaaa cgaatattta aaatgcaaca aatagagcaa agggataaaa tagcaaaagc
    20461 gtgatgaaaa tgttattttc cgatgccgca ctgacccgct acaatgtcag attactgccg
    20521 taaagaagtt tgaggaagca agacaatgag caccactatc gaaaaaatcc aacgccagat
    20581 tgctgaaaac ccgatcctgc tgtacatgaa aggttcaccg aaactgccga gctgcggttt
    20641 ctctgcccag gcagtccagg cgcttgccgc atgtggcgaa cgttttgcct atgttgatat
    20701 tctgcagaac ccggacattc gtgcggaact gcctaaatat gctaactggc cgaccttccc
    20761 gcaactgtgg gttgacggcg agctggtagg cggttgtgat atcgtgatcg aaatgtatca
    20821 gcgtggcgaa ctgcagcagc tgatcaaaga aactgccgct aaatacaagt ctgaagagcc
    20881 ggacgcggaa taatccgtcc tgcaatactg acggatgcga acgggcatcc gtcagtcctt
    20941 tctctatccc caatccagcc cttgtggcga acttgaaaat cccgcctcac gcagcagcgt
    21001 aaacatcggc gtttgccgga ccggtagatc attcacttct gttagcgtaa agcgcagccg
    21061 tggttcgcga cgcagtgcgg tagtcagcgc gtggaaaacc tctggggcga gtaattcctc
    21121 tttttcctgc cagaccagca tttttttgcc accttgcgcc agatagagta acaatttgcc
    21181 gccagaaacg accaccagcg cacccgcccg acgcgttgga accagcgttg ccggatgtgc
    21241 aggccagggc agaagatttc cccacacatt agccggatcg ttggcagaga gcgccactgg
    21301 tgtatagtgg cgcgtttgcg tggcttgtgt cgccagatcg cgcaatcggt caatagtcag
    21361 acgttcagcg aattgcgcgc cacccagacc ttctacaaaa cgacctcgca taattcgccc
    21421 ggagtcttcc atacttcgac aaagcgtttg catcgatgga aacccgtcag ggatattttc
    21481 ggctatcacc gcctgacgac tgatgatgcc gtagcggtcg agcatatttt ccgccagcgc
    21541 cagcatcctt tcggtatcgt ttagtggctc cacctgcaat aacgaccagc gtccagccag
    21601 atttggtgtg ttgtaagata ccagcggcga gacgggttgc gcatagacag gacgtccacg
    21661 acgagcccgg tgactgcggc gagttgaggt gcgtgcgttg gaactgctgc gggtgagggc
    21721 gcgtaacggt gcccaaatgt cgctggtgat gacgccttgc cagaccagcg cccataacgc
    21781 ctcttgcagg gcagagagat caaccgattc gccgattttg tcgcgtatcc gttggctgat
    21841 ttgttgtgca aaccaggctc ctccgtcagc cagaacagcg attatcgctt gctgcagcgc
    21901 cgaacgattc gcctgatccg cttcggcggg agtgaacgat tctgcggcat attcctgtag
    21961 atgcagtgcc accaggccgt catcttcacc cagctttttt tgtcccgacc agataaccgc
    22021 tccggttgcc agtaattcat cgagcatttc tggcgaatag tcgcgtacgc gggcaggcag
    22081 gatctggctt tcccagagtg aggcgggtaa accgactccg gcaagctgtt cgatcacccg
    22141 catcacgcca tctacgccct cataaacgcc tggcgatgtt gaggcaaaga gcgccgggct
    22201 accatcggtg gcgggtaata cgccctgacg ttccagcagc aatcgcgcat aggtcgtggc
    22261 tgcaacggga cgcgtcgctt ctctggcggc ttgcagcgag cgcaaacgca gacgacgaaa
    22321 tacttcatcg ctcacccaga tgtcttgttg cagattcatc accagaccct gttcacgcag
    22381 ttgctgaagc tgctcttcga caatggcaat accgagacta aactcatgag ccagttgttc
    22441 agccgtgacc agagcatgag cccggagata gcgcagaaag aggtcgcgaa gcgggtaact
    22501 gactctatgt aaataaatct ctggcaatga ctctggtagt cgtacgccga gggcatcacg
    22561 cagcctggcg gcatcatcca tacaggcaag acgctcctgt ccgctaatca tcgctgggaa
    22621 gattcgtttt actgcaagaa gattttccag atacgacgca acctcttcac tgctgcctgt
    22681 atgccgttgc gccaggtctt caacggtcat tggccccagt tcgcgcagca ggtcgaacaa
    22741 tccttcttca cctttcgctc ttctgccagg agccagtcgt tgcaactctt cttccacctg
    22801 gcgaatgacc tgcgggtcga gtaattcccc cggatcgacc tgtccgagta gattgcgcag
    22861 taactcgctg tccagcgaca gtacggatgc ccggcgctct gccagcgggg cgtcgctctg
    22921 gtacataaat tccgcgacat agccgaacaa taaacttgtg gcgaaaggcg agggcgtagt
    22981 ggtcgttaca tcggatattt gaatttcgcc accgttcagg cgacgcatca aacgttccag
    23041 tgcgggaaga tcataaacat cttgcagaca ttcgcgtagg gtttcgagaa tgaccggaaa
    23101 atccggatat ccctgagcga tttccagcaa ctgactggcg cgcagccgtt gttgccaaag
    23161 cggggtgcga tggcccggag tgcgccccgg cattaataat gcccgcgcgg tgcattcgcg
    23221 aaaacgggcg gcgaaaagtg ccgagctgcc taccgcctcg cggacaattt gcagcaactt
    23281 ttctggttca aacaaaaaaa tcgcggcgtc gggcaattta ccatcagtgt caggaatgcg
    23341 cgcaacaatg ccgtcatcac tggcgaccac agacgcgtca gcgccccata gggcatgtat
    23401 tcgcccggca atcgccagcg cccagggttc atgcacccgt cttccatagg gagagtgcaa
    23461 aataatacgc cagtcgccaa tttcatcacg gcaccgttcg aggaccagat ggcgactccc
    23521 cggaacgatg cccgtcgcgt tgcgttgctc gtcaatcaac ccctgaatat tggcgttcgt
    23581 attttcttct gccagccacg ggggaatagt cccggaaaag aacgcgccat ccgccagtaa
    23641 atgaagaaaa tcgccgatca tctcgcctaa ttcagccgga cgtccgttac cttcaccacg
    23701 ccagaagggg agccgggcag aacgacccgg agcaggagtc acaatcacct gatcgcgggt
    23761 gatttgctgg atccgccatg aggtagcgcc gagagtgata atgtcgctca cccgcgactc
    23821 atataccatc tcctcatcca gttcacccac ccgccgcgaa ccggcctgtt cttcaccttc
    23881 gggtaataac acgctataca tgccacgatc cggaatggtg ccgccgctgg taaccgccaa
    23941 caattgagcg ccaggtcggg cggtcaatat cccggtctcc ctgttccaga ccagtttggg
    24001 gcgaaaagca gaaaaatcgc cagagggata gcgcccggaa agcatatcca gcgtggcgtc
    24061 aaaaacacgt cttggcagat ctttccacgg tgcggcacgg cgtacgcggg agtaccattc
    24121 gtctacctgt aatgcatcca tcgccgcggc ggcaacggtt tgctgcgcaa ggacgtcgag
    24181 tggattatgc ggcggtgtca ggttttccag cctgcctgcg aacatacact ctacaatgac
    24241 tgcggaatcg actaagtcac gccgggtacg agggaaaaac agccctttag atacaccgcc
    24301 aacctgatgt ccggcgcgac caatgcgttg taacccactg gcaacagaaa gcggcgttgc
    24361 cacctgaatc accagatcca ccgcgcccat atcaatcccc agttcgagac tggaggttgc
    24421 gaccacgcag cgtaattcac ccgatttcag cgcctgttcg gtgattgctc gttgttcttt
    24481 agagacggag ccgtggtgtg agcgggcaat aaaaacgtcg ctactttgta catggttaga
    24541 ggttgcgccg gaggtcgact cgaaatgggc cgtatcaacg gcgatagacg gggaacgctg
    24601 taagcgtgcg gcgtaaagct catttaatcg tgccgtcagt ttttccgcca gcccacgtga
    24661 attagtaaag acaatggtcg agcgatggcg caacacttca tcaaggatac ccgtttcaat
    24721 atatggccag atggagcctt cccggccggc atggctgtct tcgccggtgc cgctggcgac
    24781 cgatgagaca tcatccatat tggcgaccgg tacgacaatt cgtatctgcg gatggcgcat
    24841 tgcgggcggg ttgactaccg taaccgggcg atcgccacca agaaatgctg ccacatcgct
    24901 ggctgagcgc acagtggcag aaaggccaat tcgctgtgct gaggtgtgga gcagcgcatc
    24961 gagccgctcc agacttaacg ccagatgcgc accacgttta ctgcccgcta ccgcgtggac
    25021 ttcatcaata attaccgttt cgacgccgcg tagcgtttcg cgcgcgcggg aggtcagcat
    25081 cagatagagt gattcgggtg tggtgatcag aatatccggc ggattacggg tgagtttgct
    25141 gcattcctgt gcaggcgtat cgccagtacg gatccctacg cgaagattga cttccgtttc
    25201 gccgcgccgc cgccgttcat cggcaatacc cttcaacggg agctgcaagt tgcgctgaac
    25261 gtcggtgccc agagctttta tcggtgaaat atagaggatg cgtgaggttt tacgcttatg
    25321 cgcctcgcgg gtatcttcgc cgccttcgcg gaagagccga tcgagggcgt agagaaatgc
    25381 tgccagcgtt ttcccggagc cggtcggtgc aatcaccagc gcatgttcgc ttcgcgccgc
    25441 cacatgccag gtttgcgact ggacagcggt cggctgttta aaggcgcgaa gaaaccagtc
    25501 gcgggtcgcc ggtgaaaaca cgtccggcag gagcgatgaa gggtctggat tatctgccat
    25561 taacgctatt ccccgtgaat atcatgcttg atgcgtggaa tagcctcact atagatgcct
    25621 gaatcattgc atgtcatcag gcatcgactc gattacacct cttcggcggc aggtagcggc
    25681 cagcctccca gacgtttcca gcggttgacg atttcacaaa acagcacagc agtgcgttcg
    25741 gtgtcgtaca gcgcggagtg cgcctgggtg ctgtcgaagt ccatgccagc ggtctggcaa
    25801 gcctttgaca atacggtttg tccgagtgcc agcccggcca gtgcagcagt gtcaaaagtg
    25861 gcgaaagggt ggaacgggtt acgtttcagt gaggcgcgtt ctgcggcggc catcataaag
    25921 ctgtgatcaa aattggcatt gtgcgccacc ataatggcgc ggttacagcc gctcgcttta
    25981 atacctttac gtacaacttt aaaaatttcg tgcagcgcct cgtattcgct gaccgcgccg
    26041 cgatcgggat cgttcgggtc aatgccgttg aaggcgaggg cctctggctg caaatttgcg
    26101 ccgacaaatg gttcgacgtg gaaatgtaat gtggtgtccg gcatcagcca gccttgttca
    26161 tccattttta gggtgatggc ggcaatctca agcagcgcat cggttttggc gttaaatccg
    26221 gctgtttcaa catcgatcac aacaggataa aaaccacgaa aacggtcgca cagaccggta
    26281 agttgagcgt tatcggacat ctgattctct taatacggat aaattgcagc gcgcattatg
    26341 acaaatatta cttgtcgatg cagtaaagat gcgggcgcga tgagttcacg cccggcagga
    26401 gattagttgc ccagaccgcg accggcgtct ttctcttcga ttaactcaat tttgtaaccg
    26461 tccggatctt ccacaaacgc gataaccgta gtaccgcctt ttaccggacc cgcttcacgg
    26521 gtcacgttac ccccgttttg acggattttt tcgcacgctt cagcggcgtt atctacgcta
    26581 agcgcgatgt gaccataagc agtgccgagt tcgtatttat ccacgcccca gttgtaggtc
    26641 agttcaatca ccgcttcttc ggtttccggg ccgtagccaa caaacgccag tgagtatttg
    26701 tattccgggt tttcgctggt acgcagcagt ttcatgccca gcactttggt ataaaaatcg
    26761 atggagcgtt gcagatcgcc aacgcgcagc atggtatgaa gaagacgcat ctttttatcc
    26821 tcaaaatggc taacagattc aaaatgttgt tttagtatag cggcggcttt acgccgctcg
    26881 caatgttgga ttacaacgtc gggtaatcgg tatagccttc cgcgccgcca ccgtagaaac
    26941 tttcggcacg ctgtgggtta agctcagctt tgcgctgcaa gcgggcgacc agatccgggt
    27001 tcgcaatcca gtcacgacca aatgccaccg catcaattaa ccctttgccg atcagcgttt
    27061 cagctttttc tactgtgtat gcacctgcgc cgataatcgg accgtggaaa cgggcgcgta
    27121 ctttttcgcg gaacgcatca gtatacggtt caccccccgc ccaatctggt tctgacatat
    27181 gcagataagc aatgccgcgt ttacccagtt gttcaatcag atacagtgca tcggcttctt
    27241 cattcgcgcc gttatccgtg ttctggaaag taccgattgg cgaaacgcga atgccaatgc
    27301 gatcggcacc ccattcttca atcccggcat cgaccacttc cagtaccaga cgtgcgcgat
    27361 tttccacgct gccgccgtac tgatcggtac gatggtttga agaaggagaa aggaactgat
    27421 gcagcaaata accgtgagca gagtggagct ctaccagatc aaaaccggct tcacgcgcgt
    27481 tagcaatggc ctgacggaaa tcattgacga tacctggaat ctcttccagt tcaagcgcac
    27541 gcggcatgga tgtttcaaca cggatcgcct gaccattttc atcgcgcaga gaagtacgtg
    27601 ttcccgcgct aagtgctgaa ggcgctaccg gtgcctgacc gccaggttgc aggctggcgt
    27661 gagaaatgcg tccggtgtgc cacagctgca cggccatatg accattttca gcatgaacgc
    27721 cagcggtgat ttttttccat gcggcaattt gctccggact atggatgcca ggcgcacctg
    27781 catatccttt tgcctgggca gaaatttgcg tggcttcact aataatcaaa ccggcactgg
    27841 cacgttggcg atagtattcc gccatcaacg gggtaggaat gtcacccggt tcaatactgc
    27901 gcagacgcgt cagcggtgcc ataaaaatac ggtttgccgc cgtgatcgcg cccactttca
    27961 gtggggaata cagtttttca gatgacataa aggctcccgg tcgtctggtg ataaaaaatg
    28021 cctgctaaac ggcaggcgtc gcaataatgt tttttacatg ggccagcgcg ttttccagtg
    28081 gctcgaaact gcgcgaaatt ttggcctgca gattcgcacc cagccatagt gcgtaaagca
    28141 cttgtgcctg ttgtagcggt tcgccacaaa aggttaaaca atggttctca cggccatttt
    28201 ccagcgcctg agagagcagg gcgatcacgc cgcgagcacc tttatccatc gcgctgcgca
    28261 tatcttctga cagatcgcac acttcggcag agagttttac tgtcaggcaa ccactgatgg
    28321 ttccatgttg gcaaaactgg ttcagtgttt gctggtaata agccagtatg cggtcgcggt
    28381 agttaccttc gccggattgc agcaactcag tcagtcgctg gtgatatgcg gcgtaatgac
    28441 gctcaagcat ggcaacgcca aacgcttctt tagagcgaaa gtagtgatag aaggaccctt
    28501 tcggcacttc agcggttttt agtaattcgc ttagccccat cccggtgaat ccacgttgca
    28561 ggcaaagttg ctcgcccgtc gccaggagat gttcgcgagt atcatgttcg gtgtgtttgt
    28621 tcatgcgttg gagtgtagta gaccagtcgg tctagtgcaa gcaaagttgc tactttggac
    28681 attaacagct tcaataatgg taataatctt atctgaggag ggcgaagtgg cggagcaatt
    28741 agagttcttt cccgtccaga gcccgtgccg gggaatttgc cagtctgacg aacgcgggtt
    28801 ttgtcgcggc tgttttcgta gtcgtgatga gcgttttaac tggaataaaa tgagcgatgg
    28861 cgaaaaacaa gaggtgctgc gcctttgtcg gcagcgatta atgcgcaaat tacgcgcaaa
    28921 taagccagcg tcatcagacg aaccggagca accatcactc ttttgaacct aatcttgcgt
    28981 atactcctct catatctgat aagaggaagt gattatggtt cagcgtatta ctattgcgcc
    29041 gcaaggcccg gagttttccc gttttgtgat gggctactgg cgattgatgg actggaatat
    29101 gtccgcccgc cagctggtca gttttattga agagcatctg gatctcggcg tgaccaccgt
    29161 ggaccatgct gatatttatg gtggctatca gtgcgaagcg gcgtttggcg aggcactgaa
    29221 actggcacct cacctgcgtg aacggatgga aatcgtcagt aaatgcggta tcgcgacgac
    29281 cgcgcgtgaa gaaaacgtca ttggtcatta catcactgac cgcgatcaca tcattaagag
    29341 cgccgaacag tcgctaatta atctcgcgac cgatcatctg gatctgctgt taatccaccg
    29401 accagacccg ttaatggatg ccgatgaagt ggcggacgcg ttcaaacatc tgcatcagag
    29461 cggcaaagtg cgtcattttg gcgtatcgaa ctttacgcct gcgcaatttg ccctgttgca
    29521 atcacgtctg ccgtttaccc ttgccactaa tcaggtggaa atatccccgg tgcatcagcc
    29581 gttactgctg gatggcacgc tcgaccaact acaacaactg cgtgttcgtc cgatggcgtg
    29641 gtcctgcctt ggtggtggtc gtctgtttaa tgatgattat ttccagcagc tgcgtgatga
    29701 actggctgtg gtggcagagg agttaaacgc gggctcgatt gaacaggtgg tttacgcctg
    29761 ggtattacgt ttaccatcgc agccgctgcc aattatcggt tcaggtaaaa ttgagcgcgt
    29821 acgggcagct gtcgaagcag aaacactgaa aatgacccgt caacaatggt ttcgtatccg
    29881 taaagcggca ctggggtacg acgtaccgta agcactttta ggaatagccg ccgttcaaaa
    29941 atgtgtcact ggtttacact tattcaggaa tgcacaatga acggaggtcc tatgaaacgt
    30001 tttagtctgg ctattctggc gctggttgtt gcaaccggcg cacaagctgc cagtgaaaaa
    30061 gtcgagatga acctcgtcac gtcgcaaggg gtagggcagt caattggtag cgtcaccatt
    30121 actgaaaccg ataaaggtct ggagttttcg cccgatctga aagcattacc ccccggtgaa
    30181 catggcttcc atattcatgc caaaggaagc tgccagccag ccaccaaaga tggcaaagcc
    30241 agcgccgcgg aatccgcagg cgggcatctt gatccacaaa ataccggtaa acatgaaggg
    30301 ccagaaggtg ccgggcattt aggcgatctg cctgcactgg tcgtcaataa tgacggcaaa
    30361 gctaccgatg ccgtcatcgc gcctcgtctg aaatcactgg atgaaatcaa agacaaagcg
    30421 ctgatggtcc acgttggcgg cgataatatg tccgatcaac ctaaaccgct gggcggtggc
    30481 ggtgaacgct atgcctgtgg tgtaattaag taattaagag gccagcgtac cttgcggtgg
    30541 tgcttgctca agttgcgaaa gcgagcagta cagccgccag acaattgccg ccagctcacg
    30601 ggcggcaggt tgatgatgac gggcaaggct gtcgcaaatc cgctgtaatt cttcaagtgt
    30661 ggccgccagt gatttttgct gaacgccacg ctcactcatc actccccgca acagtgaaat
    30721 acagttatcc cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc gcaattgcca
    30781 gacaacatga gaacagttca gcagcactac accccagcgt aataaccaac ggcgagccag
    30841 cgcatcctgg ctgttactca actgactgac gtgatgataa gtgagcgatt caaattcgct
    30901 ttcactcagt gttggatggc ggcttagctg atcgacaaaa tcccggcgca gcgcgcgaat
    30961 atggcggcgg cttttacgag catccgatcc tggacgcaga atggcgaacg ctagccaagc
    31021 cagcgcaacg ccaacgattt ttgccagatt atcgttaaga aaatcagcaa aatcatacac
    31081 cgggggatta gtgacagcga taaacgaacc cataaaaaca atcagttgcc cccacagtgc
    31141 ggcaaatttg ggcatctgca atttaagaag ctgcattgtc gccagcagtg gaaagagaaa
    31201 cagtaaaaat tgccacagat cgctaatctg gaccatcagg ccaaatttga ccacaaagct
    31261 gaatagcgaa agtaacacca gcgtgcgcat cagcagtgac aacgacttaa acggtgctgc
    31321 gacggcggag tagagtacgc agctaattgc tgccagcgtt aatgcattgg caccggcatc
    31381 ccattgcgat gcaatactcc atgcgccaat catcatcaac gtacaaaatg tacgcagccc
    31441 gctccacata gcttcggcgt tgtcggtatg acgagccaga gcggatgtcc gtgcgagtct
    31501 ggcatgatca tctacaccgc tttgcagacg atgtaattcc tgactacttt gcagataaag
    31561 gcggcaaaaa taacgtagtc gctgccagaa ggcgacgtgc cggtagtcgg cgacgttggt
    31621 cgggcgtagc ggggcgataa tacgtgcgac ggtgtaagca tctgtttgcg aactggcgag
    31681 cgccgtcagc aattgttcga gaatttctcg cgtggcacct ggcggtgagg gccagttgag
    31741 caacatacgt cgcaggctgg agatgacact ggtcatacgt aattgctggt ggagtagcgc
    31801 attaaggcgc gcgttttgct ggcgaaaacg atagtggctc cagaaagcct ggatacgcag
    31861 caaattcatg gtcagtatct gcccaatcac cccctcatgt gctgcacgaa tggcatcgtt
    31921 tgtttcaggc tgccagagta aactggcatg ttccagtaat cgggcgtgca tgtttttcaa
    31981 tgcggttaaa agggctgtag catcggaact gctaggcagg atcatcatca tcatgccgcc
    32041 gcacaaaata ccgactatca cctcgcaaac gcgcgcctga gcgatatccc acagctggct
    32101 ggcctcagta atattaacca tcggaaaggc gatgatggca gccgtgtagc ccgccagttg
    32161 aaatgcatac gcgacgttat tcgtgaagtg cgcacaggcc caggtacaaa agccaagcca
    32221 cgccgacatg ctcaatagaa aaaaccacgg ctcattgagc gtatgcccgg caagaagcag
    32281 tgccgcaatg gctcccagta aactgccagc gatgcgtccg aggcttttgc tgatgacacc
    32341 gccaacggtg ggaaagctaa ccactgcagc cgaggtcatc gcccaatagg gttcatccag
    32401 attcaaataa taggcaaccg tcagcgccag acacatggca atggtattgc gtaacgcata
    32461 acgccattgc gccagcgtgg ccttgaacca gggcaaattg cgcaaggacc atgacgatgc
    32521 gttcattatt gacctacggc aatgctacag gtggtgcccg acaccagcgt gacatcgcct
    32581 ggaactttat ccagcgcaaa gcggacggga acgcgttggg cgagacgaac ccaggggacg
    32641 ttaggtttca catccggaat aaggcttgag tcactttcaa cgctttgatc ataaatcgcc
    32701 cgaccgatac tggaaacgtg accccgtaac gttttgttat cactatagag ggtaatttgt
    32761 gctggtgcac cttcgcggat atggcgcaac ttggtttctt caaaataacc aatgacgtaa
    32821 aacgagtggc tgtcgacaag ggcaaacagc ggtttcccgg tgtcagcgta atcgccgatg
    32881 cgggtagtga ggttagtcac ccaaccactc accggggcgc gaatttccgt ttgcgccagt
    32941 tgccattgtg tctgtttcag cgtggcctgt gcggcattaa cactggcctg catcgcttta
    33001 acattgaggt tagcggtatc gagttcttcg gcagaaataa aattttgtga gagatgacga
    33061 cgacgattcg cttcgttatt ggcttttgcg aggtcagatt gcgctttcgc cagctgagcc
    33121 tgtgcgttca gctcggcgat ctgaaagggc gttttatcga tcgtgagcag aagatccccc
    33181 gcgttaacca gttggttgtc ttttatattc agctcaacaa tacgtcccga cacctgtggg
    33241 gtgatagaaa ccttttctgc gcgtattttc ccgtcgcgtg tccacggaga ttgcatgtaa
    33301 taattccaca gccaccatcc ggcaagaacc gcaactatcg ctacaatgat tgttgagaaa
    33361 tacttaattg ttttaattga catagttacc acgcaatcag tattgccaga gcaaggcaaa
    33421 cgcaaatcgc aaacagcgat aaatccatta acaagggatg ccagatgtca ccggcgtaga
    33481 tccagccacg aagcaggcgg tgtacgacaa gccagatgac gaatccaaac gcgaaggctt
    33541 tgaaaaacgg aggaaaatag acggacgcac cgaacaccag atcttgtaag ggcaatcctg
    33601 ttgcattgag cataaacttc acaaagtaga ttcctttacg accgaatata ttgcgttaga
    33661 ttaataaata ttctttaagt gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac
    33721 ggtaaccaaa tgcagcaata catttgtttt agcaatacaa ttgctgcaca ctattctaaa
    33781 agccgcataa tatcttagca agctaattat aaggagatga aattggaatc gccactaggt
    33841 tctgatctgg cacggttggt gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg
    33901 ctggagttaa cacaaaccca ttgggttacg ttacacaata tccatcagtt acctccagac
    33961 cagtcgcaaa ttcaactggc aaaagcgatt ggcatcgagc agccatcact ggtccgtact
    34021 ctggaccaac tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg
    34081 gctaaacgta ttaaactgac ggaaaaggca gagccactga tcagcgaaat ggaagctgtt
    34141 attaacaaaa cccgcgcgga aatattacat ggcatctccg cagaggaact ggagcaactg
    34201 attacgctca tcgcaaaact tgagcataat atcattgagt tacaggccaa agggtgaaat
    34261 gaagggggcg tgtggtcagt aacctgacca cacgcaaact tatttagcgc ggagaaacgg
    34321 tcacctgact gccattgctg gccagtacga cacgttggcc cggagagaaa cgagtgttgc
    34381 cttgtttctg taccaccatg atggtattac catcgtcttt acgaatttcc agctcgacac
    34441 cctgcgtttt gttcattgca ctctgtacgc cctgaccagc tacgccacct gcaacagcgc
    34501 ctgctgcagt agccagagaa cgcccggttc cgccaccaac agtattcccc aggaaaccac
    34561 caagaacagc accgccaatt gcaccgataa cgttggaatc atcaccgccc tgaatctgta
    34621 ccggacgtac gttaacgatg gtgccatagc tgacattctg tacttgtttc gcttcagaag
    34681 cggtataaac atcccctgac agggtgtcgt tattaacaca accgacaaga gacagaccta
    34741 ccattgaaac aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac catgaaacgc
    34801 cacgcaagca tccctcatgg tcaaagtata tggcatatgt gaatccatgg ttacatgttc
    34861 taccaaatat gagcaaatca taatgaaagt aggatcaacc aattataaac aaaacattca
    34921 tgatgattat tgatttgaag ataatgtttt actaccaacg gtaaaaatcg gtcattccgc
    34981 atggtattca cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg aaaaaggatg
    35041 aactatgaaa tcgggccgct ttattggcgt tatgtcaggc accagccttg atggtgttga
    35101 tgttgtgttg gcgacaatag atgaacaccg ggtcgcacag ctggcaagct tgagctggcc
    35161 gatcccggtg tctctgaaac aggctgtact ggatatttgc cagggccagc agcttacact
    35221 ttcgcagttt ggacagcttg atactcaact cgggcgactt tttgctgatg cggtcaatgc
    35281 cttgcttaag gaacaaaacc tgcaggcgag agatatagtt gcgatcggct gtcacggtca
    35341 aaccgtctgg catgaaccga cgggcgtgga accacacact ttacagattg gcgataacaa
    35401 tcaaattgtg gcacgcaccg gaattacggt tgtcggtgat tttcgccgtc gcgatattgc
    35461 cttgggagga caaggcgcac cgctggtacc tgcgttccat catgccttgc tggctcaccc
    35521 aaccgagcga cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac tgctcattcc
    35581 tgggcagccg gttggcggct acgataccgg tcctggtaac atgctgatgg atgcctggat
    35641 ctggcgtcag gccggtaaac cttacgataa agatgccgag tgggcacggg cgggtaaagt
    35701 tattctccca ctgctgcaaa atatgctcag cgacccatat ttctcgcaac ctgcaccgaa
    35761 aagcaccgga cgcgaatact ttaactacgg ctggctggag cgccatttac gccattttcc
    35821 gggtgttgat ccccgtgatg ttcaggccac actggcagaa ctcaccgccg tgaccatttc
    35881 tgaacaagtt ttgttgagcg gtggctgcga acgattgatg gtatgtggtg gaggtagtcg
    35941 taatccgcta ctcatggcgc gtctggcggc attactgcca ggcacagaag tcaccaccac
    36001 cgatgccgtt ggcattagtg gcgatgacat ggaagcattg gctttcgcct ggcttgcctg
    36061 gcggacgctg gcgggattac caggaaatct gccttccgtc actggcgcaa gccaggagac
    36121 ggtactgggg gctattttcc ctgctaaccc gtgatatcag agttaactga atttttctta
    36181 ccgcgtctgc cgttacactt ggaacgttag ggagggcgta ttgccctcca gaccaggaaa
    36241 gtcttcggga aatgtctatg aaaaaactgt tactgatctg tttgccggtg cttctcaccg
    36301 gctgtagcgc gttcaatcaa ctggttgagc acatgcaaac cgctacgctg gaataccagt
    36361 gtgatgaaaa accgttgacg gtcaaactga ataatccgcg ccaggaagtc agttttgttt
    36421 acgataatca actactgcat ctcaaacagg gcatttcagc gtctggtgca cgttacactg
    36481 acggaatcta tgttttctgg tcgaaaggcg atgaagcgac tgtctataaa cgcgaccgca
    36541 tcgtattgaa taactgtcag ttacaaaatc cacagcgttg agatttttcc aggggcggcg
    36601 cacaatagcg tcacccactg acaatccgta aagaaaacca tgtctgataa cgacgaattg
    36661 cagcaaatcg cgcatctgcg ccgtgaatac accaaaggcg ggttacgccg tcgcgatctt
    36721 cccgccgatc cattaaccct ttttgaacgt tggctctctc aggcgtgtga agccaaactg
    36781 gcagacccta ccgcgatggt ggtcgctacc gtggatgaac atgctcagcc ttatcaacgc
    36841 atcgttttac tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa cctcggtagc
    36901 cgtaaagcac atcaaattga aaataatccg cgcgttagcc tgctgttccc gtggcatacc
    36961 cttgaacgcc aggtgatggt gatcggtaaa gcagaacgac tttcgactct cgaagtgatg
    37021 aaatattttc atagccgccc gcgtgatagc cagattggtg catgggtttc gaagcagtcc
    37081 agtcgcattt ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa gcagaagttt
    37141 caacagggcg aagtgccatt gcccagcttt tggggcggtt ttcgcgtcag ccttgaacag
    37201 attgagttct ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta ccagcgagaa
    37261 aatgatgcgt ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat cttgctttaa
    37321 tcgctggtac tcctgattct ggcactttat tctatgtctc tttcgcatct ggcgaaaagt
    37381 cgtgtaccgg caaaggtgca gtcgttatat acatggagat tttgatggca agcagtaact
    37441 tgattaaaca attgcaagag cgggggctgg tagcccaggt gacggacgag gaagcgttag
    37501 cagagcgact ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct accgctgaca
    37561 gcttgcattt ggggcatctt gttccattgt tatgcctgaa acgcttccag caggcgggcc
    37621 acaagccggt tgcgctggta ggcggcgcga cgggtctgat tggcgacccg agcttcaaag
    37681 ctgccgagcg taagctgaac accgaagaaa ctgttcagga gtgggtggac aaaatccgta
    37741 agcaggttgc cccgttcctc gatttcgact gtggagaaaa ctctgctatc gcggcgaaca
    37801 actatgactg gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt ggcaaacact
    37861 tctccgttaa ccagatgatc aacaaagaag cggttaagca gcgtctcaac cgtgaagatc
    37921 aggggatttc gttcactgag ttttcctaca acctgttgca gggttatgac ttcgcctgtc
    37981 tgaacaaaca gtacggtgtg gtgctgcaaa ttggtggttc tgaccagtgg ggtaacatca
    38041 cttctggtat cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc ctgaccgttc
    38101 cgctgatcac taaagcagat ggcaccaaat ttggtaaaac tgaaggcggc gcagtctggt
    38161 tggatccgaa gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac actgcggatg
    38221 ccgacgttta ccgcttcctg aagttcttca cctttatgag cattgaagag atcaacgccc
    38281 tggaagaaga agataaaaac agcggtaaag caccgcgcgc ccagtatgta ctggcggagc
    38341 aggtgactcg tctggttcac ggtgaagaag gtttacaggc ggcaaaacgt attaccgaat
    38401 gcctgttcag cggttctttg agtgcgctga gtgaagcgga cttcgaacag ctggcgcagg
    38461 acggcgtacc gatggttgag atggaaaagg gcgcagacct gatgcaggcg ctggtcgatt
    38521 ctgaactgca accttcccgt ggtcaggcgc gtaaaactat cgcctccaat gccatcacca
    38581 ttaacggtga aaaacagtcc gatcctgaat acttctttaa agaagaagat cgtctgtttg
    38641 gtcgttttac cttactgcgt cgcggcaaaa agaattactg tctgatttgc tggaaataat
    38701 gcattaagtg gaaaggggga gtgagaaatc actccccctg gtttttatac agggaacatg
    38761 atgaaaaata ttctcgctat ccagtctcac gttgtttatg gtcatgcggg taacagtgcg
    38821 gcagagtttc ctatgcgccg cctgggcgcg aacgtctggc cgctgaacac cgttcaattt
    38881 tctaatcaca cccaatacgg caaatggact ggctgcgtga tgccgcccag ccatttaacc
    38941 gaaattgtgc aaggcattgc cgccattgat aaattacaca cctgtgatgc cgtgttaagt
    39001 ggctatctgg gatcggcgga gcagggtgaa catatcctcg gtatcgtccg tcaggtgaaa
    39061 gccgcgaatc cgcaggcgaa atatttttgc gatccggtaa tgggtcatcc ggaaaaaggc
    39121 tgtatcgttg caccgggtgt cgcagagttt catgtgcggc acggtttgcc tgccagcgat
    39181 atcattgcgc cgaatctggt tgagctggaa atactctgtg agcatgcggt aaataacgtc
    39241 gaagaagcgg ttctggcagc gcgcgaactc attgcgcaag ggccacaaat tgtgttggtt
    39301 aaacacctgg cgcgagctgg ctacagccgt gaccgttttg aaatgctgct ggtcaccgcc
    39361 gatgaagcct ggcatatcag ccgtccgctg gtggattttg gtatgcgcca gccggtaggt
    39421 gttggtgatg tgacgagcgg tttactgctg gtgaaactgc ttcagggggc aacgctgcag
    39481 gaggcgctgg aacatgtgac cgctgcagtc tacgaaatca tggtgaccac caaagcaatg
    39541 caggaatatg agctgcaagt ggtggctgct caggatcgta ttgccaaacc agaacattac
    39601 ttcagcgcaa caaagctctg aaatatctca tttcggccca caacgacctg tgggccgatg
    39661 gatattactt taagccttcc gctgacagcg cgtcttgtac ttccggacgt tcagccatac
    39721 gttgcataaa tgctgcaatg tgctctaacc cttccagatt cagtttcacc gcgtatgccc
    39781 agcgcagaac cgtaaacaga taggcatcag caattgtaaa tctttgcccg cagatccagt
    39841 gctcatcctt cagtgcctcg ttcacatatt gcagcttctt ctccagctgc gcgcgaactg
    39901 tcggtttgta ctcttccggt gtatcagggc gaaacagagg tgtgaaacct ttatgcagct
    39961 cggtggcgat gtaattcagc cattcgatgg ttttatagcg ggaaatactg tttaccggtg
    40021 ccagcaactg gcggtcgggg acgctgtcgg caagatactg cataatcgct acgccttccg
    40081 tcagcaaagt accgtcatcc agcagcaatg caggcacctg ccccttaggg ttaacggcaa
    40141 agtaatcgtc accgttttcg agacgttttt tcattaaatc cacactgacg agggtaaaat
    40201 cctttccgct ctcacgcagg gtgatatggg aagcgagaga gcaggcaccc ggtttgtaga
    40261 acaatttcat cggtaactcc ttttgtctgt ggtttaaagt atggtagtgc gttgctgaac
    40321 aaaaaaaaag ccgctaacga aaagttagcg gcttgtaaaa gagtttccct gaattttacg
    40381 ctacagcagc tttcgccgct ttgtctgcag cgtcatcctg cgtcatacgg tgcagtttag
    40441 gcgcggtcag cagcatcagt actgcaataa tggcagtagc gacaccaatc tgcaagaata
    40501 cgcgaccata aacttccagt gacatcagcg ggtcggtaac gttatccggc acagccatca
    40561 tacccgcaac ataaccacca atcaggtttg caccggcagt ggtcaggaac cagctaccca
    40621 taatgaagcc catcagacgc tgcggaacga gttgtgcaac cattgccaga cccagaccag
    40681 agatcatcag ttccccgatg ctctgcaggc catagcttgc gaccagccag cttacagaca
    40741 cgataccagc gtcagacgcg aatttcgcac ccagcggcag aatcaggaac gcaccagaac
    40801 acatcaccat gccgattgca aacttggttg gcatcggcag ggtatcgccc atcttgttat
    40861 agatagcggc cagaatcgga ctaccgatga tgatccagaa cgggttcagt gcctgatact
    40921 gctcaggttc tacggccaga cccagaatgg agtgctcaac gttacgaatc gcaaagaagt
    40981 tcagtgatgt cggcatctgg ctgtacagca cgaagaagat gattgcttcg agcatcagga
    41041 tgaaggcaac gatcatttta cgacgcgcag cacctttcat cgcgaaggct tctttaccga
    41101 agataaccac gataccgaag gcaacaacgc ccagcgccat acgcgcaact tcctgattgt
    41161 gcagcagcca ggtggcgata gcgatcagtg ccacaacacc aataatggtt agcagcaggt
    41221 tacggtagtt gataggctcg aagtctggtt ttgaaccgta ctgtttaacc cagcgttggc
    41281 agaaggcgaa gttaacgata gtgatcagca ggcctacaac gctcaatgca aacgcaacgc
    41341 tccagccgta tttcgcggcc agccacggcg tagcaatcat agagaagaaa gagccgatgt
    41401 tgacggacat gtagtacatg gtgaatgcac cgtccagacg cgggtcgttt ttctcatagc
    41461 atgtagaaag cagagaagac gggttagcct taaacaggcc gttaccgacc gcaatagccg
    41521 ccatacccat ataaacaata ccggcgtcgt gaccagacca ggcaaccaga gcataaccaa
    41581 tcgccagcac aatagcgccg agcataatta cgcgtttagt acccagtacc ttgtcaccta
    41641 accagccgcc gatagcgacc agaccataaa ccagggcact aaaggaagag aaaagggtga
    41701 ttgaatccgc ttcagacata cccagttgtt taaccaggta aacagccata attccttgta
    41761 ggccgtaata accaaaacgt tcccataact caatcgagaa gatgagatag aacgccttcg
    41821 gttgtttgaa agcgttcaaa ctgacgcttt cagttggttt ttggtttgca gtggacactt
    41881 ttacctcttt ttttacatcc catattaacg ggggtgcttt ctcgcgttat gtctaaaaaa
    41941 catacgcgca attgttatag tgggagggga aacggcaggt aatgttcact atcttgcact
    42001 ttcagacaag tcctttgtaa taatctgtta catataactg aggcggggaa ttcaaccatt
    42061 ccgggaaatg ttatccagcg ttaaattttt caaaaaacgt aacggcagat tttttcactg
    42121 cactgagagg ggggtgatgg cttaacggcg atatgttctg ctatttagcg tttttactag
    42181 tgagatagtc cagtttctga aaaatagcca gtgtaatgtt ttgtaggtca atatttgttg
    42241 cgtgtgtaaa tttaaatgat tttaacctga tttttgcaat gtttttacat tgccacagca
    42301 tcgtgatctt gatcacgaat gaatagaaaa tttcgctatt aaaaaaacaa ttaacctgca
    42361 tttttggtta tccgcaaacg gattacctga tgataacgct aagcaatggc atcattcacg
    42421 ttaaaaagat gttttaaaag aggataaaga aaggttatca atggggtaat cggtgttacc
    42481 ccttttcttc agatgtcaac tttctctttg tattcacaaa gatcttcaat aatacaagag
    42541 ccacagcggg gcttgcgggc aatgcaggta taacgcccgt gcaggatcaa ccaatggtgg
    42601 cagtcgactt taaactctgc cggaaccact tttagtagct tttcttctac ctgttcgaca
    42661 tttttccccg gcgcaaattg agtacgatta caaacgcgga aaatgtgcgt gtcgacagca
    42721 atagtcggcc agccgaatgc agtgtttaat acgacgttgg ctgttttacg acctacgccg
    42781 ggcagggctt caagcgcagc acgatcttcc ggaaccaagc cattatgctg ctccagtaag
    42841 atacggcagg ttttgatgat attttctgct ttgctgttat aaagcccaat cgttttgata
    42901 taggttttaa ccccttcaac gcccagttca agcatcgctg caggcgtatt cgccaccggg
    42961 tagagtttcg ccgtcgcctt attaacactg acatcggtcg cctgagcgga aagcagtacg
    43021 gcaatcagca attcaaaagg cgaactgaaa ttaagctcgg tggtgggatg aggattgttc
    43081 tcacgcaggc gagtgaggat ctctaggcgt tttgctttgt tcatcagaca ttccctgttt
    43141 caccgtttgg caatgcacgt tcggcagctg cttcagtacg gcgctttttc attctttcat
    43201 caatcaggta ttttcctgcc agcatcagtc ccaggccaat aaatgcacct ggaggcagca
    43261 tcgccagcag gaaaggggag tcggtgtgga aaatttccac gcgtaatact tttgcccagc
    43321 tacctaacag cgcatctgca ccgtcaaaca atgtgccatt gccgataatt tcgcgtagtg
    43381 aacccagcac gaacatggcg caggttgcgc ccataccaat tgaaaagccg tccagtgccg
    43441 aaagcgccgg accttttttg gcggcgaagg cttcagcgcg gcccacaacg atacagttag
    43501 tgacaatcag cggaataaaa atccctaatg attgatacag gccaaaggcg taggcgttga
    43561 tcagcatctg tacagcgctg accaccgagg cgatgatcat cacgtaaatg ggaatgcgga
    43621 tctcggctgg cgtccagtga cgcagcgtcg aaatggtcag gttggtcagc gtcagtacca
    43681 gcgtagtcgc aagtcctaaa cccagagcgt tagtggcagt ggacgtgacc gccaacagag
    43741 gacaaaggcc gagcaactgg accagcgcag agttgttttt ccacaacccc tgaacaataa
    43801 cgtctttaat ttcgctcacg ctttattctc cacaggcagg aagttgagaa agttgtgccg
    43861 gtaacgtctg agcgtacaat ccggcgcgtt ttaccgcatt aaccaccgcg cggggagtaa
    43921 tcgtcgcgcc ggtgaactgg tcgaaatcac caccatcttt cttcaccgcc cagtgcgcat
    43981 catctgcacc actgattttt ttacccgcaa aatgggtgat ccagtcagaa aggcgcagtt
    44041 cgattttatc gccaagccct ggcgtttcgt ggtgctctgt cacgcgcgtg ccaagtaccg
    44101 tgccgttaaa atcggctccc accagcagct gaatcgcacc ggaatagcca tctggcgcgg
    44161 ttgcttccag aacggcggct accggtttgt catcctgttt ggcgatgtaa acccgatgct
    44221 cacctttacc taactctggc gcagttacca gatagcaact ctgtgccagc gcattgttat
    44281 agcgttcggc tggcagcacc tgatcaaata atgccttttg ttgcagactg gcctgttcag
    44341 caatcgtcgt tttggtcatc tggttgatgg ccgcagttaa ccctgttgaa cccgctgcaa
    44401 atagcgctaa cgtaatgccg tgttttcgga tagttttcag catggtttac cctttgcgat
    44461 ggccgtagac gcgcggacgc gtgtagtaat cgatcagagg aaccgtgatg ttcgccagca
    44521 ggacggcaaa agccacgccg tcaggatagc cgccgaaact gcggatcaac cagactaata
    44581 agcccgccag cgcgccgaaa ataagacgac cacgattggt cgtagaagcg gtaaccgggt
    44641 cagtcaaaat aaagaatgcg ccgagcatgg tcgcaccaga cagcagatga atttgcggtg
    44701 ctgccagtgt ttctggtgag aacaaccagc ccaacgttgc gcataacgcc agcgttacta
    44761 agaagctgag gggaatatgc cagcgaatcg ctttctgcca tagcaaccac aggccgccag
    44821 ccagccaggc gagatttacc cattgccaac cagcgcccgc cagaataccg ctgtagatcg
    44881 gatattgcat aatctgttca accgaatgac cggcacggac agaggtttta aatgtatcca
    44941 gcggtgtcgc ctgactaatg ccatcaatac ctaagcgtag tgtgttcata tcaccaccac
    45001 tggcggtatg tccgctgaaa ataacctgta tggcgtcgat aaaaccaggg atgttgaccg
    45061 caatttcatg tggcggtaac cagctggtca tctgcaccgg gaaggagatc agtaagacca
    45121 cataaccaat cattgccgga ttaaacgggt tttgccccag accgccatac aactgtttag
    45181 cgatgatcac cgcaaacacc gtacccagca cgaccatcca ccatggcgcg agggggggaa
    45241 tacttaccgc cagcaataag cctgtcagca atgctgagtt atctttcaac gttgcggcta
    45301 ccgactgctt gcgtagtttg agtacgagag cttcggctaa cagagcacta accgatgcca
    45361 acaggatctg aacgagagta ccccaaccaa aaaaccacag ttgcgctgcg attcctggca
    45421 cggctgcgag caacaccaac agcataatgc gcgatgtctg gcgctggtta tgggtataag
    45481 gggagctagc tattctgaat accatttagt cctcgtttac aaccttctgc tgggcggctt
    45541 ttttggcctg agcgcgggca atagccgcgg caactgccgc tttgcgcgga tcaacctgtt
    45601 cttctggttc tgcgttagtc tgttgctgtt ccagcttgcg cgctttggca cgggcaatag
    45661 cggcttcgac ggcggctttg cgcggatcaa cctgttcttc tggttccgca ttagcctgtt
    45721 gctgttccag tttgcgcgct ttggctcggg caatagcggc ttcgacggcg gctttgcgcg
    45781 gatcaacctg ttcttctggt tccgcattag
//
LOCUS       DAZKGS010000018        44338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKGS010000018 DAZKGS010000000
VERSION     DAZKGS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..932)
                     /gene="prmA"
                     /locus_tag="ACHOQY_001064"
     CDS             complement(51..932)
                     /gene="prmA"
                     /locus_tag="ACHOQY_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9677207.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(944..2395)
                     /gene="panF"
                     /locus_tag="ACHOQY_001065"
     CDS             complement(944..2395)
                     /gene="panF"
                     /locus_tag="ACHOQY_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9677208.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2385..2627)
                     /gene="yhdT"
                     /locus_tag="ACHOQY_001066"
     CDS             complement(2385..2627)
                     /gene="yhdT"
                     /locus_tag="ACHOQY_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9677209.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2720..3655)
                     /locus_tag="ACHOQY_001067"
     CDS             complement(2720..3655)
                     /locus_tag="ACHOQY_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9677210.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(3687..4517)
                     /locus_tag="ACHOQY_001068"
     CDS             complement(3687..4517)
                     /locus_tag="ACHOQY_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9677211.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            4859..5713
                     /locus_tag="ACHOQY_001069"
     CDS             4859..5713
                     /locus_tag="ACHOQY_001069"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFP9677212.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            5749..6639
                     /locus_tag="ACHOQY_001070"
     CDS             5749..6639
                     /locus_tag="ACHOQY_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9677213.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            6700..8199
                     /locus_tag="ACHOQY_001071"
     CDS             6700..8199
                     /locus_tag="ACHOQY_001071"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9677214.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            8200..9189
                     /locus_tag="ACHOQY_001072"
     CDS             8200..9189
                     /locus_tag="ACHOQY_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9677215.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            9211..10173
                     /locus_tag="ACHOQY_001073"
     CDS             9211..10173
                     /locus_tag="ACHOQY_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9677216.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            10170..11216
                     /locus_tag="ACHOQY_001074"
     CDS             10170..11216
                     /locus_tag="ACHOQY_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9677217.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(11308..12657)
                     /gene="accC"
                     /locus_tag="ACHOQY_001075"
     CDS             complement(11308..12657)
                     /gene="accC"
                     /locus_tag="ACHOQY_001075"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9677218.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12668..13138)
                     /gene="accB"
                     /locus_tag="ACHOQY_001076"
     CDS             complement(12668..13138)
                     /gene="accB"
                     /locus_tag="ACHOQY_001076"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9677219.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(14004..14834)
                     /locus_tag="ACHOQY_001077"
     CDS             complement(14004..14834)
                     /locus_tag="ACHOQY_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677220.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(14871..15845)
                     /gene="yhdH"
                     /locus_tag="ACHOQY_001078"
     CDS             complement(14871..15845)
                     /gene="yhdH"
                     /locus_tag="ACHOQY_001078"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9677221.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEDDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15997..17937
                     /gene="csrD"
                     /locus_tag="ACHOQY_001079"
     CDS             15997..17937
                     /gene="csrD"
                     /locus_tag="ACHOQY_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9677222.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            18242..19285
                     /gene="mreB"
                     /locus_tag="ACHOQY_001080"
     CDS             18242..19285
                     /gene="mreB"
                     /locus_tag="ACHOQY_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9677223.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            19351..20454
                     /gene="mreC"
                     /locus_tag="ACHOQY_001081"
     CDS             19351..20454
                     /gene="mreC"
                     /locus_tag="ACHOQY_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9677224.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            20454..20942
                     /gene="mreD"
                     /locus_tag="ACHOQY_001082"
     CDS             20454..20942
                     /gene="mreD"
                     /locus_tag="ACHOQY_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9677225.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            20951..21544
                     /gene="yhdE"
                     /locus_tag="ACHOQY_001083"
     CDS             20951..21544
                     /gene="yhdE"
                     /locus_tag="ACHOQY_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9677226.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            21534..23003
                     /gene="rng"
                     /locus_tag="ACHOQY_001084"
     CDS             21534..23003
                     /gene="rng"
                     /locus_tag="ACHOQY_001084"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9677227.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            23071..26871
                     /gene="yhdP"
                     /locus_tag="ACHOQY_001085"
     CDS             23071..26871
                     /gene="yhdP"
                     /locus_tag="ACHOQY_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9677228.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            27027..28472
                     /gene="tldD"
                     /locus_tag="ACHOQY_001086"
     CDS             27027..28472
                     /gene="tldD"
                     /locus_tag="ACHOQY_001086"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9677229.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(28600..29529)
                     /gene="aaeR"
                     /locus_tag="ACHOQY_001087"
     CDS             complement(28600..29529)
                     /gene="aaeR"
                     /locus_tag="ACHOQY_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9677230.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            29712..29915
                     /gene="aaeX"
                     /locus_tag="ACHOQY_001088"
     CDS             29712..29915
                     /gene="aaeX"
                     /locus_tag="ACHOQY_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9677231.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            29923..30855
                     /gene="aaeA"
                     /locus_tag="ACHOQY_001089"
     CDS             29923..30855
                     /gene="aaeA"
                     /locus_tag="ACHOQY_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9677232.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            30861..32828
                     /gene="aaeB"
                     /locus_tag="ACHOQY_001090"
     CDS             30861..32828
                     /gene="aaeB"
                     /locus_tag="ACHOQY_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9677233.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            32920..33192
                     /gene="yhcO"
                     /locus_tag="ACHOQY_001091"
     CDS             32920..33192
                     /gene="yhcO"
                     /locus_tag="ACHOQY_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9677234.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(33248..33511)
                     /gene="yhcN"
                     /locus_tag="ACHOQY_001092"
     CDS             complement(33248..33511)
                     /gene="yhcN"
                     /locus_tag="ACHOQY_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9677235.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(33876..34346)
                     /gene="argR"
                     /locus_tag="ACHOQY_001093"
     CDS             complement(33876..34346)
                     /gene="argR"
                     /locus_tag="ACHOQY_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9677236.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            34781..35719
                     /gene="mdh"
                     /locus_tag="ACHOQY_001094"
     CDS             34781..35719
                     /gene="mdh"
                     /locus_tag="ACHOQY_001094"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9677237.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(35782..36849)
                     /gene="degS"
                     /locus_tag="ACHOQY_001095"
     CDS             complement(35782..36849)
                     /gene="degS"
                     /locus_tag="ACHOQY_001095"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9677238.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(36939..38306)
                     /gene="degQ"
                     /locus_tag="ACHOQY_001096"
     CDS             complement(36939..38306)
                     /gene="degQ"
                     /locus_tag="ACHOQY_001096"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9677239.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(38460..38858)
                     /gene="zapG"
                     /locus_tag="ACHOQY_001097"
     CDS             complement(38460..38858)
                     /gene="zapG"
                     /locus_tag="ACHOQY_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9677240.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            39052..40179
                     /gene="zapE"
                     /locus_tag="ACHOQY_001098"
     CDS             39052..40179
                     /gene="zapE"
                     /locus_tag="ACHOQY_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9677241.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            40398..40826
                     /gene="rplM"
                     /locus_tag="ACHOQY_001099"
     CDS             40398..40826
                     /gene="rplM"
                     /locus_tag="ACHOQY_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9677242.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            40842..41234
                     /gene="rpsI"
                     /locus_tag="ACHOQY_001100"
     CDS             40842..41234
                     /gene="rpsI"
                     /locus_tag="ACHOQY_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9677243.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            41629..42267
                     /gene="sspA"
                     /locus_tag="ACHOQY_001101"
     CDS             41629..42267
                     /gene="sspA"
                     /locus_tag="ACHOQY_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9677244.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            42273..42770
                     /gene="sspB"
                     /locus_tag="ACHOQY_001102"
     CDS             42273..42770
                     /gene="sspB"
                     /locus_tag="ACHOQY_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9677245.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(42813..44180)
                     /gene="dcuC"
                     /locus_tag="ACHOQY_001103"
     CDS             complement(42813..44180)
                     /gene="dcuC"
                     /locus_tag="ACHOQY_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9677246.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT        10927 a        10765 c        11638 g        11008 t
ORIGIN      
        1 tgtgagcttg ctcgcacttc gccccgcgtc accctacggc gatgcgaagg ttaattctta
       61 cgaccggtaa tacggcacca ctcttctttt tccacgaccg ggtccagtgc gaagctatcg
      121 gcataagctt cacaaacgct ctctgcctgg cttgccagaa taccggaaag gcccagcaaa
      181 ccgcctgaaa ccggcaggac gctgattaac ggtgccagtt cacgtaatgg gcctgcaagg
      241 atgttagcga ccaccacgtc ggctttcatt tcttctggct gatctttcgg taagtagagt
      301 tccagacggt cagaaacgcc attacgttcg gcgttgtcgc ggctggcctg aatcgcctgc
      361 ggatcgatat caataccaat ggcttttgct gcacccagtt tcagcgccgc gatcgccaga
      421 atgccggaac cacagccaaa gtcgatgact gttttaccgg ttaaatcgag gctgtcgagc
      481 cattgcaggc acagagaggt ggttggatgg gtacccgtac caaacgccag ccctggatct
      541 aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc agctagggca gatccacagt
      601 cgttcaccaa agcgcatcgg gtggaaatta tccatccatt cgcgctccca gtctttatct
      661 tctagttgtt cgattttatg cgcgaagcct gcgccgagca gcggatggtt ttccagaatc
      721 gccaccacgt cgttcatatc ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc
      781 cacaggcgcg tttcgcccgg cagcggctca aatactggcg tatcgtgggt atcctgaaaa
      841 gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa gatcttccgc gttcgcgccg
      901 gtggtgttca gtttcagttg gatccaaggc atggcaaaac tctttattta tcagtagtca
      961 aaacggtagc ttgcgggacg gatgtaccga aacggtttcc gaccaggaaa gccagcaaac
     1021 ttagtagtaa cgagggcaca atagggtgga agcccaggta ctgaatattc agcgtcgcga
     1081 gtacggcata cagcacgccg ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc
     1141 gttcccagta aagacccagc accagcggcc acaggaaaac ggcttccagc ccaccgaagg
     1201 ccagcaaatt cagccagatg atcatttctg gcggcttcca ggcggcaagc agcagcaacg
     1261 cgccgagaac taacgtaatt accgccgaca tccgcttcag acgcgtctcg ttttgcattt
     1321 gatccggacg gatattcaga tagagatctt taatgatcgt agcggaactt tgcagcaact
     1381 gggcattgat cgtcgacatg atcgcagcca taggtgcagc caggaagatt ccggcagcaa
     1441 acggtggcag cacttttacc attaacgtcg ggatcaccag atccggtacg gtgagatcgg
     1501 ggatcactgc acgacctaac gctccggcca ggtgcatacc gaacatcagg attgcgacta
     1561 caatcgtacc gatgatgatc ccccggtgta ctgctttgct gtctttataa gagatacagc
     1621 gcaccgcagt atgcggcagg ccaatcacgc caaagcacac cagtacccag aacgacgtca
     1681 taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg
     1741 tttgcaaggt ctgtactgcg ttgcttaagc cgccagcggc atgtaccacg ccaataagca
     1801 gcacaacggt gccaatcagc atcacaagcc cttgcatggt gtcgttcagc acgctggcgc
     1861 gaaagccacc aaaggcggta tataacgcaa tgctgatacc aaaaatcagc agcccggttt
     1921 cataaggaat acccgccgcg gtttccagca ggcgcgcacc gccgataaac tgcacggtca
     1981 tcgcaccaac gaacgcaacc agcaaactca aactcgccag ccacaccaga agacgactct
     2041 ggtagcgggc aaacagcata tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg
     2101 caaacttctt gccgagaata ccgagtgaaa gccagactgc aggaagctga atcatcgcca
     2161 gcaataccca gcccagcccg tatttataag cagctcctgg cccgccgata aacgaactgg
     2221 cactgatata ggtcgcggtg agcgtcatcg ccagcacaat accgcccata gagcggctgc
     2281 cgaggaaata ctcattaagg aaggtgccgg tgctccgttt acgcatcgca taaaccgaga
     2341 taccgaacac caccaccaga taggcgacca gcggtagaat tacttcaagc tgcatcgtca
     2401 tcctccagtg ggatatcgcg atagataaat ttcaccatcg cccagcacag tccaataaac
     2461 agcagcggcg tcaggatgca ggccatctca aaccagcgcg gaaagccggt aaaaccgggg
     2521 gcaacgccag gtaagtaagc ggctactaac caaactgcca gatacaaaag ggtcagcccc
     2581 agcgcccagc gcgcctcttt atgggcctga acaaaacgag tgtccacgga agggttcctc
     2641 ctcatggggg gatacgagaa acgggcaatt ctacggcaac tcttcaggca gggaaaataa
     2701 tctgacagaa aattgccgtt cagtgctgct gcaaaaagtc ttcaatgtca gccagtgagg
     2761 atatcccttc catcggacct tgccgcatta cggcgagcgc gccgctggca tttgcatatt
     2821 gcagtgcctt atgtgccggg aaaccggata agaaaagagt gataaacgtt gcgccaaagc
     2881 agtcgcctgc acccgttgga tcgataattt cgatatcgtg gcctgtaaca tgctgggcgt
     2941 gtaatgtacc gtttttaagc ttgtaatagc tggccccacg ctgggcgcgt tttatcgcca
     3001 catgttttac gccgccgtgg agaagatcgc taacaatctg ttcttctgac agatttttgt
     3061 gacgcgcgaa gaaagggagt tcgctttcgc tggggatgaa aatatccgta tattcaatca
     3121 aataatcgag agcctgcgcc atttcaggaa tgctcagcat ctctttgcga atattgggat
     3181 cgaaagaaac ggtgccgcca gccgatttga tcgtcgttat tgctttacgc atgacatcga
     3241 tcatgcgaaa tgagaacaac gatgagccca caatatggag atggttacac tgttttagca
     3301 gatccttatc aatgtgctca gcagtaaaca agccgcaggc gctgttaggg atattaaaga
     3361 cgaaatcgcg ctgctgggga ttttgataac tcacgaacgc cgtacctgtg acggcattgt
     3421 tcataacatg gatcccatcg gtaatgacac cttcatgacg gaggcgctct ataatgagtc
     3481 ggccaaaatc atcattacca acacatccga agaagatggc ccgatgggac agttttgcca
     3541 cctgagcagc aaaaattgct ggcgcaccgc tgggaaatgg cccgataaac tccccaggct
     3601 gagtgaattt ttgatgtggg ttgcgggaca aaaactcggc cagcaattcg cccatagcgc
     3661 agatagttat tgtcacaaaa cccctctcat tcctgaagcc tttcgcgatt ggaccaggta
     3721 aagcactcat tactggtata aaggataacg aaatcagccg actgcaattg ttgctgatat
     3781 tgttcgttaa tgctgcggct gacgatcaca ttttctgcct gatgcaaatg cccgatagca
     3841 gccgttgatg gcgcaggatc ggcggcaacg ataaccgtct tattctgggc atgatcgaaa
     3901 cagagctgcc aggcagcggc cagtctggga acgttgatat ttaaagtacc atcagcatcc
     3961 agatggctga tttccagaat gctatgtgta atggtgaaat gctgaagtgc ctgttcaagc
     4021 ggttttccgg ccagtgcggt gtcggggcgc agcatttcac tgccagccag tattatttcg
     4081 ccatcaataa actgatttgc cagcagcgca tgttcgggat cgttggtgat cagacgcagt
     4141 tttttcactt cgctgagcaa tggaatgatc ttacggcaga ttgtcccatg tccaagaaat
     4201 agcgtgtcac gatcgcgaat taaccgtgcg caatgtcgtg ctatttccat ttgttgtgcc
     4261 agagacaatg gcgcgatcgg ttgtatcgct tctggttcag aggagagtgg tgtcgcaata
     4321 gcaccgccaa atgatcgctt taaatatccc tgttgctcca gataactgag atcgccgcgg
     4381 atcgtcacgc cagagacacc aagttgcgca gataagtcat caactttgat ttcaccttgg
     4441 gcggcaacaa tttctgcaat ttttaatctg cgtttcagta ggttagacga ttttctcggc
     4501 acgttaacac tcttcattgg cctttaatgc gtaacactat agccccttgt atttttcttt
     4561 gaaagttatc cttcaataat atcgacaatt cgaaaagaaa atcatgggcc aaaaatcgca
     4621 acaaaaatag attaattcat tgagaaataa atgaatttat tcatatttat gcagagatgt
     4681 tattggtttc acttgtaaag tgaaaaacaa attaagttat tttttgtaag ttattttgat
     4741 gatattcgac atgcatcacg ctttttgccg cttttgctgt gctagtttgc tttcaaaatg
     4801 aaagtgaata ttctctttct tcattttgta aggcgaacga caaataacag gagctgcgat
     4861 gtacatcatt tcaagcaaaa acatgctgaa aaaagcccag tcagagggat atgccgtccc
     4921 cgctttcaac attcataacc ttgaaacttt acaggtagtt gttgagagcg ctgcacaact
     4981 tcgctctccc gttatgctgg cgggaacacc cggtacttat cgttatggtg gcgtgggaag
     5041 tttaatttcg ttagtacaat cgctggctcg cgagtacaac ctgccgttag tcctgcatct
     5101 cgaccatcat gaagagagtg atgacatttt taacaaggtt cgcgcaggga tccgttctgt
     5161 catgattgat ggctcccatt tgccatttga agacaacatt gcgcgagtgc aagaagttac
     5221 caccttttgc catcgctttg atgttagcgt tgaggccgag ctagggcgtc ttggcggcca
     5281 ggaggacgat ctgctggtgg atgaaaaaga ttccgcttat acctcaccag ccgcagctgc
     5341 agagtttgtt caacgtaccc aaattgactc cctggccgta gcaatcggca cagcacatgg
     5401 tttatatgcg catgaaccac gactggattt tgtgcggctg gaacagattc gtcagcgagt
     5461 ggagattcca ctggtgttgc atggtgcttc gggcttatca gaacaggacg ttcagcactg
     5521 tattcgccgc ggtatttgta aagtgaatgt cgcgacagaa ttgaaaattg cgtttgccga
     5581 tgcgttaaaa ggatatttct acaacaatcc tgcggcaaat gatccacgcc actatatgca
     5641 gcccgcaaag gccgccatga aagaagtcgt aactcgtatt attggtgtct gtggtagtga
     5701 aggaaaaatt taagacgttg taccctacaa aaacaatgag gattgtttat gaaaaatatt
     5761 aaattacttg gttcagctat gttggtgagc ctggcattgt tctcgcaaaa ttcgttggca
     5821 aaagagtata aaattggtgc gtcattatta acacagcaac atcctttcta tattgatctt
     5881 gcgaatgcca tgaaagaaga ggcaaaaaaa gacaatgtca atttaagtgt ctctattgcc
     5941 aatcaggatt taaacaaaca actttctgat gttgaggatt ttattactaa aaaagtggat
     6001 gccattatta tctctccggt cgattcgaaa ggcgtccagg cggcgataat taaagccgat
     6061 aaggccggta ttcccgttat caccgtcgac gtcgctgcgg aaggcgttcc ggtcgtatcg
     6121 catgttgcga ccgataacta tgcgggaggc gttgaagcag gcaagttaat gggcaaactt
     6181 ttgaatggta aaggtactgt agccattatt tcttatccgg cactacaatc ggtggttgcg
     6241 cgcgttgatg gttttaagaa agggttaagc gatacaccgg atattaaaat tgttgctgaa
     6301 cagcctggta ttacgcgggc tgaagcgtta acgactgcgc aaaacattat gcaggcaaat
     6361 gccaacctga atggtctgtt tggctttggt gatgatgcgg cgctggcagc ggttatcgca
     6421 gcaaaatctg cgcataacga caacatcaaa atcattggtt ttgacggcat gaaagaagcg
     6481 cgtgatgccg ttgacagtga aaaaacgttt gcggccgtga ttcggcaata tcccgatcaa
     6541 atgggtgcta aagccattga tgccgctgtt gatcacctca atggtaaacc ggtagaaaaa
     6601 atgatcccgg tacaacccgg cgtttataca ggtaaatagt aaacatcttc acctcacaac
     6661 aaaacgttat tggcgttttg ttgtgacacc gaggtcatta tgacggatgt tattcttgat
     6721 gtttcgcata ttgcgaagac atttggtcat gttcaagcat taaaagatat taccctctct
     6781 ttacgtaaag ggcgggtgca tactttatta ggcgagaatg gtgccggtaa atcgacatta
     6841 atgaaaatac tggccggtgt ttatccgcca acccagggaa cgataacact acgtggtgaa
     6901 actatcacca ttaataatcc gcagcattca cgccagttag gaattgcgat tatttttcag
     6961 gaattgagcc tgagtaataa tatgaccgtg gcggaaaata tttatgccaa taatgaaccg
     7021 cgacgttttg gcattattaa cgataaaaaa atgctcgcag attgccagaa tctcctcgcg
     7081 gaactgggca taccactaga cccactagaa atggtcggca atatgtcgat ggcacatagg
     7141 caactggtag aaattgctaa agccttaagc tatgcagcgg atgtcgtgat tatggatgaa
     7201 ccgacatctt cgctaagtga taatgaagcg gagattctgt ttaacattat tgaaaaatta
     7261 aagcagcgag gctgtgcggt tatttatatc tctcatcgca tggatgaaat tatgcggatt
     7321 tcagatgata tttcggtgat ccgcgacggt gagtatatcg ccacgcatga aaagaaaaac
     7381 agtgatatcc agcatttgat tgcccaaatg gttggtcggg aaatgaaaaa tatttggcca
     7441 gcacgtcttg gggaaatacc ggatgaaaat gttcctgcaa aactggaagt aaaaaatctt
     7501 tcccatcctt cgctttttaa agagatcagt ttcgctgtgc gtccggggga ggtcctcgga
     7561 ttttttggcc tggtgggggc cgggcgttcc gatgtgatga aggcgctctt tggcctcgtt
     7621 tcgtatcagg ggactgtgtt gattgatggt aaggaagtca ggatcgctaa tcctaaacag
     7681 gctattgatc atggcattgc ttttgtgacc gaaaaccgca aggaagaagg gttagtgctg
     7741 atgcacgacg tcaatatgaa cacgcaccat gtggcgtttc aatataacgc ttcgcggatg
     7801 ggactcatca atcaccgaca agaagaggcg aagacgctgc aatcaattgc cagaatgaac
     7861 actaaagtga gttctgtaca tcaggcagtg ggagcgctga gcggcggtaa tcaacagaaa
     7921 atcgtgttat cgaagtggct ggagaaaact ccgcgtattt tattgctcga tgagccaacg
     7981 cgcggagtgg atgtcggcgc aaaatttgaa atctataacg tgatacgtca actggctgcg
     8041 gcagggacag caattattct ggtgtcctct gaactgccag aagtcatggc acttagcgat
     8101 cgtctggtgg taatgcgtaa taaaacgatt gccgatattt attcatgtga aaatttaacg
     8161 cagacacagg taatgaccgc tgcaacagga gtacgttaaa tgacagagaa aactcttgcc
     8221 agtccgaaac cttcggaaat tcccattgca aataatttta aaaaagtctt caggcaatat
     8281 ggcggtatcc tcagtggaat ggtggtgtta atcattctgt tcagttttat taatgacagt
     8341 ttttttaccg ccaacaacat tactaatatt attcttcagg tttcgataat tgcaattacc
     8401 gcctatggaa tgacttatgt acttttgctt ggcgatattg atttatcggt cggttcaacc
     8461 attgcattga ttggcacatt tgctgcgctt ggggcttcat ggggaattcc atttatttta
     8521 ttggtcccat tatccattat tgcagcactg gctctgggga tgataaatgg tggcttgacg
     8581 gctatagcgg ggatcccgtc gtttattgtc actgttgcta ctatggggat cttccgtggt
     8641 atcgcttata tagtgaccga tggtatgccc atcatgatta aagatgatgc ctttctggca
     8701 ctggggaatg gagaatttct ctatatacct attccaatct ggatattaat catcttattg
     8761 ctgattaacc acttcattct tactaaaaca acctttggac gaaaaattta tatcactggt
     8821 ggtaataaag aagcggcaat ttattcaggg ataaatgtca cccgcttgaa aattaaagtc
     8881 tttatgatta cagccgtgct ggcaggtatc agcgggatga tcctcgcctc acgccgctat
     8941 tccgggcaac cgaattccgc gttgagctac gaactggatg ctattgctgc ggctgtactg
     9001 ggaggcacca gcctgaacgg cggctatgga accgtggtgg ggacggtgat cggcgcatta
     9061 actatcggcg ttattaataa tggcatgaac ttaatgaatg ttccctattt ttaccagatg
     9121 gtggttaaag gattagtcat tcttgtagcc gtttattttg acgtaagaaa taaaagaaaa
     9181 cgcagctaat catttcaagg aattaatcat atgagtaagg tcttcacaat tggagaaatt
     9241 ctggttgaaa ttatggcgag taaaatcggc cagccatttg atcaaccagg tatctggaac
     9301 ggcccttatc ccagtggtgc gcccgctatt tttatcgacc aggtgacacg tcttggcgtt
     9361 ccgtgcggca tcattagctg tgtggggaat gatggctttg gtgatattaa tatacatcgt
     9421 ctggctgctg acggtgtgga tatcaggggg atttcagtct tacctctgga agctaccggg
     9481 agtgcctttg ttacttatca taactctggc gatcgcgatt ttatttttaa tattaagaat
     9541 gccgcttgcg gtaaattatc ggcgcagcat gttgatgaaa atattctgaa ggactgtacc
     9601 cattttcata ttatgggctc ttccctgttt tcgtttcata tggtagacgc agtaaaaaaa
     9661 gcggtaacta tcgttaaagc aaatggtggt gttatttctt tcgaccctaa tattcgtaaa
     9721 gagatgctcg atattccaga aatgcgtgac gccctccact ttgttcttga gttaacggat
     9781 atctatatgc ccagcgaggg cgaagtgttg ctcttgtcgc cacattcgac gccggagcgt
     9841 gcaatcgcag gttttcttga agaaggtgtt aaagaggtga ttgtgaagcg cggcaatcaa
     9901 ggtgccagtt actactccgc gaatgagcag tttcatgttg agagctatcc ggtggaagaa
     9961 gttgacccga ccggtgcggg agactgcttt ggtggcgcgt ggattgcctg ccgccagcta
    10021 gggtttgacg cccatcgagc gctgcaatat gccaatgcct gcggtgcgct ggcggtaacc
    10081 agacgtggcc caatggaggg aacgtctcgc cttgcggaaa tcgaagcgtt catccagcgc
    10141 catgacatgt ccattcggga agcagcacaa tgaaaaacga aatttggtcg tggattggcg
    10201 atctctcgca ggttgccgga attaaacact atgagttacg tagtggcaga gcaaagggta
    10261 cagaagcgtt tgatgtccgt actggtgcgg gactggcatt tacggtggta aaagatcgcg
    10321 cgctggatat tgcatgggcc agctacaagg acacggcgct ttcttttatc acccccaacg
    10381 gcattgtcgc ccccgctttt tttgagtctc agggcaacgg atttttacgt agcttttacg
    10441 cggggttact gactacctgc ggacttagct acattggcac gccttgtgag gatgaaggag
    10501 aaacgctggg gttacacggg cgtttggctg cgactccagc agaagaggtg gggtatcgca
    10561 cagaacggac cgacgacggc attgaatttg tcatcaatgg caaagtacgg gaaacgcgac
    10621 tgttcggcga aaatctgacg ctggaaagga ccattcgctg ccgttatggc gaaaatgtgc
    10681 tgcgcattga agacaaggtg actaatcatg gtttcacccg ccagccgctg caaattcttt
    10741 atcactttaa ttacggctgg cctttattat cgccacaggc agaaatcttg ctgtcggcta
    10801 aaagcgtaac gccacgaact ccgcatgcgg cggaagggtt aaccagtcat ctggagattt
    10861 gtaccccaca gcccggtttt gacgagcagg tttactatct cacgttgaat agcgacagtc
    10921 aggggatgag taaagttgct ctggtgaacg ccgaactggg gtggggaatt tacgagaaat
    10981 tcgacaccat gcagctaccc aactttatcc agtggaaaaa tttaggcgcg ggtgagtatg
    11041 ttatgggact ggaggtcagt aatagttttc ctgacggtcg tgataaagaa cgagcgcagg
    11101 ggcgtctgcc ctttattgag ccaggggaaa caaagaaata ctgctttgag ctgggaattg
    11161 ttgatggcga tgctgaaatg agcgcgttga aagcggaaat tgcaggctac cgttagctaa
    11221 cggcaataaa aacgccgggc agcgttaaca ggcccggcgt tgattacgtt ctttgctaag
    11281 cgcgatgaaa acaccgcgcg tcacaaatta tttttcctga agaccgagtt ttttctccag
    11341 atagtggatg ttagtgccac catgctggaa gttctcgtca ttcatgatgc ggatctgcag
    11401 atcaacgttg gttttgatac cgtcgatgat cagctcctgc agcgcattct tcatgcgggc
    11461 aatcgccacg tcacggtttt caccgtagca aatcagctta ccgatcattg agtcatagta
    11521 cggcggtacg gtgtagcccg cgtagatatg agactcccaa cgtacgccaa aaccgccagg
    11581 tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag gtgttcggat cttcggcgtt
    11641 gatacgacat tccaccgcat ggccgcgaac gtgaacttct tcttgcttga tcgacagcgg
    11701 ttgaccggca gcgatacgca gctgttcttt gatcaggtca acgccggtga tcatttctgt
    11761 aaccgggtgt tctacctgaa tacgggtgtt catttcgatg aaatagaact cgccgttttc
    11821 gaacaggaac tcgaaagtac ctgcaccgcg atagccgata tcaacacacg ctttagcgca
    11881 acgttcgccg atgtagcgac gcagttccgg ggtaatgccc ggtgctggtg cttcttcgac
    11941 cactttctgg tggcggcgct gcatggagca gtcacgttcc gccagataga tagcgttgcc
    12001 ctgaccgtca gccagtacct gaatctcgac gtggcgagga ttttccaggt atttctccat
    12061 gtaaaccata tcgttgctga aagcagcttt cgcttccgca cgggtcatgg agatggattg
    12121 tgccagttca gcgtcgccgc gcactacgcg cataccgcga ccaccgccgc cgccggaggc
    12181 tttgataatc accggataac caatgcgttt agcaatggca cggtttttat ccatatcgtc
    12241 gcccaacggg ccgtcagaac ccggtacgca agggacgccc gcttttttca tcgccgcgat
    12301 tgcggatact ttgtcgccca tcaggcgaat ggtttctgct ttcgggccga tgaagataaa
    12361 gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg gagaggaagc cgtaacccgg
    12421 atggattgct actgcgccgg tgatttcagc ggcgctgatg attgccggga tgttcagata
    12481 actttttact gacggagcag ggccaataca gaccgtttca tctgccagta atacgtgttt
    12541 tagatcgcga tccgcgctgg agtgcacagc gacagtcttg atgcccagtt ctttacaggc
    12601 acgaagaata cgcaatgcaa tctcgccgcg gttggcaata acaattttat ccagcatgtt
    12661 cgcctcgtta ctcgatgacg accagcggct cgtcaaattc taccggttgt ccactttcga
    12721 ccagaattgc tttcacggta ccggatttgt ccgcttcgat ctggttcatc attttcatgg
    12781 cttcaacgat gcacagggta tcgcccacgt tgactttctg acccacttcg atgaacgctt
    12841 ttgcgtccgg gcttggggtg cggtagaaag taccaaccat cggggaacgt acgatgtgac
    12901 cactgatttc cgctgctgct ggcgcttcca tggaaggaac ggtcgccgga gcggctgcgt
    12961 tagattgagc tggctgctgc attattggtg cagcgtaagc ttgttgcatc acagggaaac
    13021 ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc gccttcagaa atttccagtt
    13081 cggagatgcc tgattcttca accagctcga tcagtttttt aatcttacga atatccatga
    13141 gtgggttccg tactctttgt ttagtgtgat tgtgacaggc gtttcaccgc cgtctgtaaa
    13201 gcgtatgccc cgacagctgt atgcatagcg ataaattcca gcaggccggg acgccggtct
    13261 attttgcccc agagtgtcaa tgttagactt gacggacatt gtgcagcgat ctgctgctat
    13321 cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg tcaccttttc cgcagcgcaa
    13381 aaactgcgtc agggaggacg aggtcgcaca ttataacgat ttcgtagcaa ttggcagcta
    13441 aatactggtc ttatcaggga agataatcaa cagctaacat gtaaataacc ttcaacaccg
    13501 tgtaatttgc aacaagccgc acaattcacg aaattagcgc catcatcgac ggaacttctt
    13561 ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag atgaggggct gcaatgagat
    13621 aatcttcaca gaccacaaat aatgtaggga tgccaggatc gcgacaagat agacgacatt
    13681 acgcagcggt tgctggggat tacccaattt tcactgcatt gtctggatag atgttaatgc
    13741 taaagcacgc aaaattaacc gattgacaat gctcagcatt aaataggttc gagtgattaa
    13801 tttgtagtca aacaaaatca gattattccc cccattttgc agaaggtaaa ctgttttgtc
    13861 aggcggcagt gcggctatct gatgctttca gaatttgcca cctgacaata aaaaagccga
    13921 tttggtagca aaatcggctt cggtgagttt gcagttttca tcacatactc cgacaagctt
    13981 tttcaagcat gacggaggtt gcgttaatgc aaggtttcta cccagggata aaagtgaccg
    14041 ttacggcgga tgaatatctg tgttgtcaga ggatggtcat cctcacggaa gcattgtttt
    14101 gttagctgtc gtaatgcact gagatttgct ggctgatcat tgtgggtgaa aaacagtttt
    14161 cctctggagg gaacggtcac taacaggtcg ccgcattcag gatcgggtga ttgcacccaa
    14221 agatcgtcaa tgagcatcag actgggctca taagtacccc ctgcggagat aacggcaact
    14281 tttccatcgt ttgcgcctgt atcaacgatt tcagccgtaa cttccggcaa tatttgcttc
    14341 aaattactga tggcttgttc gcggtcaatg gtcacttcgt agcgcatcag atagcgcatg
    14401 gtttcgctac catcttcagc aaacaagata attaactcat cgttaaagtc ttcccaggca
    14461 aaatccagcg aggcgttgag gacctctgga tcagactcat actggctaag ctgttggcga
    14521 acggtatcca gccaggcttt ttctttaatc accggaacaa tacagcgccc ggtcggcagc
    14581 atttctgcaa gctgtttcag catatctgca gccacttctt gcagcgggtc tggtgcggct
    14641 tgataacgtt tgtaggcatc atcaagggtg atttccaccg tttggtgacc cgtatcaatg
    14701 cgcatcaatt tgtggtcagt cgaaatcatg actttccagt ttggatgatg ttgtacaact
    14761 tcctgaccaa ggtgttcgac gaaagctgtc cggctaaccg cttttcccgt taaccgattg
    14821 aataatgatg tcaaaatgag ttccctctct tattattcct tgctaaatgg ttagttaacc
    14881 ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg cctcggcaaa gttcggtgcc
    14941 tctgacagag atatctcttt tgccgcctgg gtatagaatg attccggtaa atcggcgacc
    15001 agtcgctgcc aggcttgtgc gcggcgttct ggtggcgtca ttactgaatc caccccttgc
    15061 aaacggacat tacgcagaat aaatggcatg accgtggttg gcagagtaaa accacccgcc
    15121 agaccacagg cagccacgca gccgccgtaa ttcatttgcg ccagcacttt tgccagcact
    15181 ttgtcgccaa cagtgtcaat tgccccagcc cagacttgtt tttccagagg acgggattcg
    15241 gcaaactcat cacgagggag aatacgactg gcacctaaac ttttcagata ttcatgggta
    15301 ctttcgcgac cggaaacggc aacgacctga taacccaact tatgcagcag cgccacggcg
    15361 gtactgccga cgccaccact ggcacccgtc acgacaatct ccccgtcctg cgggcgaaca
    15421 ccggcatctt ccagcgccat cacacacagc atggcggtaa aaccggcagt accgataatc
    15481 attgctttac gcgcgtccag cccttgcggc atggcaacca gccagtcacc tttcactcgc
    15541 gcctgctccg ccagcccacc ccagtggttt tcaccaacgc cccagccagt gagtaacacc
    15601 tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta cagttccggc aaaatcgatc
    15661 ccaggaatca tcggaaaatt acggatgatt tttcccttac cggtaatcgc cagcgcatct
    15721 ttatagttca ggctcgacca gtgaacatca accgtgacat cgtcctccgg caggcgactt
    15781 tcgtcaagtg tctgtaccga tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc
    15841 tgcataagtg gtcctcatgt gcatgatgga ttggaaaata atttctgaag actatactcg
    15901 ctaatggaaa agcaaagccg atgaagcgca ataaattgcg agataaatct gatttgctag
    15961 tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac gaaattttcg
    16021 gcctttgtta cgctgctcac cgggttaaca atttttgtga ctttgctggg ctgttcgcta
    16081 agtttctaca acgcaattca gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg
    16141 attgataccc accttgtgtc gaatgacttc agcacattaa ggccacaaat taccgaatta
    16201 atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg gtgataaaca ggtttatacc
    16261 ctggccagaa atggtagtta tcgtccggtt ggctccagcg atctgtttcg cgaactgagt
    16321 gttccgttga taaagcatcc ggggatgtcg ctgcgtctgg tttatcagga tccgatgggc
    16381 aactatttcc attctttaat gactaccgcg ccgctcacgg gggcgattgg ctttatcatt
    16441 gttatgctct tcctggcggt acgctggtta caacggcaac ttgccgggca agaattgctg
    16501 gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt gggaaccatc
    16561 tatgaatggc cgcccagaac cagcagtgcg ctagatacgc tgcttcgtga aattcagaac
    16621 gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc ccaggacgtg
    16681 aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt actggaagat
    16741 caggagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga tttcaatatg
    16801 ttgagcgata cttgggggca cagccaggtt gaagaacagt tcttcactct gacgaatctg
    16861 ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca ccgcagtgat
    16921 tttgctgcgc tgttaccgca ccggacgtta aaagaggcag agagcatcgc cggtcagtta
    16981 atcaaagccg tcgatacctt gccgaacaat aaaatgctcg atcgcgacga tatgatccac
    17041 attggtatct gcgcctggcg tagtggtcag gataccgagc aggtaatgga acatgcagag
    17101 tctgccacgc gtaatgcggg attgcagggc ggcaatagct gggctattta cgatgactcg
    17161 ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca aatgctcagt
    17221 cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat
    17281 cgcgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc ggagtatatg
    17341 ccgatggtct tgcagtttgg cttatcggaa gagtatgacc gtctgcaaat cagccgtctt
    17401 attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac cgttgagtcg
    17461 ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca
    17521 caacgaaaac gcataattat tgaacttgca gaggccgatg taggtcaaca tatcagtcgt
    17581 ttacaacctg ttattcgttt agtgaatgct ttaggggtac gggtagccgt caaccaggct
    17641 ggtttgacgc tggtaagcac cagttggatc aaagaactta atgttgagtt actcaagctc
    17701 catccggggc tggtcagaaa cattgagaag cgaacggaga accagctgct ggttcaaagc
    17761 ctggtggaag cctgctccgg gaccagcacc caagtttacg ccaccggtgt gcgttcgcga
    17821 agcgagtggc agaccctgat tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg
    17881 tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc ggtttaacct
    17941 gccgtttaat ctgttttcac gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt
    18001 ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg ttgcaaacag
    18061 gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt tgtaacaaag
    18121 gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg
    18181 tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat tatcccttag
    18241 tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc tgggtactgc
    18301 gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt ccgtggtggc
    18361 cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa
    18421 gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga aagacggcgt
    18481 tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag
    18541 caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca
    18601 ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct
    18661 gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg
    18721 ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct ccttgaacgg
    18781 cgtggtttac tcctcttccg tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa
    18841 ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc accgcagaac gtatcaagca
    18901 cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa
    18961 cctggcagaa ggtgttccac gcggttttac cctgaactcc aacgaaatcc tcgaagcact
    19021 gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc
    19081 ggaactggct tccgacatct ccgagcgcgg catggtgctc accggtggtg gcgcactgct
    19141 gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg ttgctgaaga
    19201 cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg
    19261 cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaaatgtct gtttaccctg
    19321 cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg tggcccgtcg
    19381 ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat tgccgacagc
    19441 cgcctgggga cgttcagtca aatccgtact tatatggata ccgccgtcag tcctttctac
    19501 tttgtttcca atgctcctcg tgaattgctg gatggcgtat cgcagacgct ggcctcgcgt
    19561 gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa
    19621 ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc
    19681 ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt taacgatcct
    19741 tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga aggccagccg
    19801 gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact gaccagtcgc
    19861 gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg caacgatatc
    19921 cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca tctgccagcg
    19981 aatacggata ttcgtgttgg tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg
    20041 gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg cgcttatact
    20101 gtgattcagg cgcgtccgac tgcaggactg caacgtttgc gttatctgct gctgctgtgg
    20161 ggcgcggatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg tgttgctaat
    20221 gaacgtctga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat ggggccaaag
    20281 ttacctgaac cggcaacggg gatcgctcag ccgactccgc agcaaccgac gacaggaaat
    20341 gcagctactg cgcctgctgc gcccacacag cctgctgcta atcgctctcc acaaagggct
    20401 acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga
    20461 gctatcgtag ccagggacgt tgggtaatct ggctctcttt cctcattgcg ctgttgctgc
    20521 aaatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg ttactcatct
    20581 tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt gggcacaggt tttgtgatgg
    20641 gtgccatact ggatctgatc agcggctcga cgcttggcgt acgcgtattg gcgatgagca
    20701 tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca ttatggcagc
    20761 aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc tgggcagagt
    20821 ttttagtgat taacgtctct ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg
    20881 tgctctggcc gtggattttc ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat
    20941 aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc aggagttact
    21001 tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc attgaggagc agcgtcagcc
    21061 gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc gagaaagcac aggcgggtgt
    21121 cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggat actatcgtta tcctgaatgg
    21181 agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc
    21241 gggtcagacc catcaggtga tgacggcagt ggcgttggcc gacagccagc acattctcga
    21301 ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca tcgcgggcta
    21361 tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac ggtattcagg ggctgggtgg
    21421 ctgttttgtc aggaagataa atggcagcta tcacgccgta gtcggcttac cgctggttga
    21481 aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata aacatgacgg
    21541 ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt ggcgtatatt gatggcggta
    21601 ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca
    21661 agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat attgggctgg
    21721 ataaagccgc gtttcttcat gcatccgata tcatgccgca caccgaatgt gtggcgggtg
    21781 aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc
    21841 tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg accaccgata
    21901 tcacgctgcc ttctcgctat ctggtgttta tgccaggggc ttctcacgtt ggggtttccc
    21961 aacgtattga aagcgaatca gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg
    22021 acgagcaggg cgggtttatc atccgtaccg cagcggaagg ggttggcgag gctgaactgg
    22081 cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt aaaaaacgcc
    22141 cgcagacccg ttatcagctg tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt
    22201 tcgccgacgc cgaactggac cgcattcgcg ttgactcacg cctgacttac gaagcattgc
    22261 tggagtttac ctcggagtac attcccgaga tgaccagcaa gctggagcat tacaccggac
    22321 gccagccgat tttcgatctc tttgatgtcg aaaacgaaat ccagcgagcg ctggaacgca
    22381 aagtagaact gaaatccggt ggttatctga ttatcgacca gaccgaagcg atgaccaccg
    22441 tggacatcaa taccggagcg tttgtcggtc atcgcaatct ggacgacacc attttcaata
    22501 ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgtaat ctgggtggga
    22561 ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgccgc gtgctgcact
    22621 cgctggagca ggcgttgagc aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc
    22681 tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac gtactgtgta
    22741 acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg
    22801 aaatcatgcg cgagattgtt cgtgtccacc atgcttacga ctccgaccgt ttcctggtct
    22861 atgcatctcc ggcagtagct gaagccttga aaggcgaaga gtcacactcg ctggcggaag
    22921 tggaaatttt cgttggcaaa caggttaaag tacaaattga accgctctat aaccaggagc
    22981 agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa gggtttttga
    23041 gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat tttactgctt
    23101 actggagccg cgctcgttgt gatcgctgct ctgctggtta gcggcctgcg tattgcttta
    23161 ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc gactggcatg
    23221 ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggcccgac gcttgaagca
    23281 cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg cgttactctg
    23341 gcgctggatg tctggcagag cctgttacat atgcgctggc agtttcgcga cctcactttc
    23401 tggcagctgc gctttcgcac caacactcct atcaccagcg gtggtagcga tgacagtctg
    23461 gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga tctccgcgac
    23521 agtgaagtca gttttctgac gccatccggt cagcgcgccg agctggcgat cccacaactc
    23581 acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt
    23641 accggacagc acggcgtgat gcaggtgcgc atggatttgc gcgatgatga ggggttgtta
    23701 agcaatggtc gcgtctggct acaggcggat gacatcgacc tgaagccgtg gctcggtaaa
    23761 tggatgcagg acaatattgc tctggaaacg gcacagttct cccttgaagg ctggatgacg
    23821 atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg tgccagctgg
    23881 ttgggcgaga agcaaacgca tacgctgtcg gtggataatc tgaccgcgca tattacgcgt
    23941 gaaaatccgg gctggcagtt ctctattccc gatacacgga tcacgatgga cggcaaaccc
    24001 tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg cggcaaagac
    24061 aataaacgca gtgacgaact ccggattcgc gccagtaatc tggagctggc aggcctggag
    24121 ggcatacgcc cgctggccgc gaaactttca cctgcactgg gtgatgtttg gcgctccaca
    24181 caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc ggcagacaag
    24241 acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt attaccgggt
    24301 gcggaacact tctccgggac gctttccggc agcgttgaaa atggtttgct taccgcgtcg
    24361 atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactgga aatcgccgac
    24421 ggccaggcaa ctataagctg gctgaacaat gacaaaggtt tccagctgga tgggcgtaat
    24481 attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct
    24541 aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg ttcacaagcc
    24601 tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattactt aagtggcgcg
    24661 attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc gcaactcttc
    24721 ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt
    24781 gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga ctttattaac
    24841 gacggtttat ggatgaaaac cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc
    24901 accgcagtga tccctgacta ctcgaaagaa aaactgctga ttgacgctga cattaaaggt
    24961 ccgggtaaag ccgttggccc ttactttgat gagacaccgc tgaaagattc tctgggtgcg
    25021 accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct tgatatcccg
    25081 ctgaacggcg aactggtaac cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt
    25141 atcaaaccac tcgacagcac cctgaaaaat ttgagcggta aattcagctt tatcaatggc
    25201 gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa cgtggatttt
    25261 tccaccaaag aaggggcaaa agcctaccag gttgcggtga atctcaacgg taactggcaa
    25321 ccggcgaaaa ccagcgttct gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg
    25381 tgggatggta aagtgggcat tgatctgcct tatcatgctg gcgcgaccta taacgtagag
    25441 ctgaacggcg atctgaataa tgtgagtagt cacttacctt caccgttagc caaacctgcg
    25501 ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt tgaattaacc
    25561 ggacaggctg gtgcggataa ccatttcaat agccgctggt tgcttggtca aaagctgacg
    25621 ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt
    25681 ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt ggctggccct gttccagaaa
    25741 ggcgctgcgg agagtgtcgg tggtgcagcg agtttcccac aacacataac gttacgtacg
    25801 cctatgttgt cactgggaaa tcagcaatgg aataacctga gtattgtttc gcaaccgacg
    25861 gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt
    25921 aataacgcgc cgtggctggc gaatatcaaa tatctttatt acaacccgag cgtggcgaaa
    25981 actcgtggtg attcaacgcc gtcatcacct ttcccgacaa cggagcgcat taacttccgt
    26041 ggctggccgg acgcacaaat acgatgcaca gagtgctggt tctgggggca aaaattcggt
    26101 cgcattgaca gtgatatcac catttctggc gatacgttaa cgctgaccaa tggactgatt
    26161 gatactggtt tctcgcggct tactgccgat ggtgaatggg ttaataatcc ggagaatgaa
    26221 cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg atgccgccgc agaatttttt
    26281 ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt acactggcgc
    26341 aaagcaccct ggcaaccaga tgaggcgacg ttgaatggca tcattcatac tcaactgggt
    26401 aaaggcgaaa ttaccgaaat caataccgga catgccgggc aattgctgcg cttattgagc
    26461 gttgatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg cgaagggttc
    26521 tattttgact ccattcgcag caccgcgtgg attaaagacg gggttatgca caccgacgac
    26581 acgctggtgg atggcctgga ggcggatatc gccatgaaag ggtcggtaaa tctggtacgt
    26641 cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt gggcgtggct
    26701 gcggcttttg cggttaaccc cattgttggc gcggcagtat ttgcagccag taaagtgctg
    26761 gggccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc gctggacgat
    26821 ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag
    26881 ggcgcgtaat tgccccaatc tcatatgata atcgttgcca aaggccaacg agccggaaca
    26941 taactgtagg ccggataggg cgttcacgcc gcatccggca gccgtaaaaa atcctctact
    27001 gcagtaacta acgagtagca aaaacgatga gtctgaacct ggtaagtgaa caattgctag
    27061 cggcgaacgg cctgaaacat caggacttgt tcgcgatcct cggtcaactg gccgaacgtc
    27121 gccttgatta tggcgatctc tattttcagt cgagctatca cgaatcctgg gttttagaag
    27181 accgcattat taaagatggt tcttacaaca tcgatcaggg cgttggtgtg cgtgcaatca
    27241 gtggtgaaaa aaccggattt gcttacgctg accaaatcag cctgctggcg ctggaacaga
    27301 gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga tggcaaagta cagacgctgg
    27361 gcgcggtaga gcatagcccg ttgtatacct cggtagatcc gctgcaaagc atgagccgtg
    27421 aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc ccgcgaagcg gacaagcgcg
    27481 tacaagaagt gactgccagc ctcagcggcg tttatgaatt aattctggtt gcggccaccg
    27541 acggcacgct ggcggcggat gttcgtccgc tggtgcgtct ttccgtgagc gttctggtcg
    27601 aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt
    27661 tcttccttgc cgatcttgac ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc
    27721 gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc gggcaccatg ccggtagtac
    27781 ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg
    27841 acttcaactg ccgcggcact tcagtattta gtgggcaggt cggggagctg gtggcttcag
    27901 aactgtgtac cgtggttgat gacggcacga tggtcgatcg ccgtggttcg gtggcgattg
    27961 atgacgaagg tacgccaggc cagtacaacg tgctgattga gaacggcatt ctgaaaggct
    28021 acatgcagga taaactcaac gcgcgtttga tggggatgac gccgactggc aacggtcgcc
    28081 gtgaatccta cgcccatctg cccatgccgc gtatgaccaa cacctatatg ctgccgggta
    28141 aatcgacccc gcaggaaatt attgaatccg ttgagtacgg tatctatgca ccgaactttg
    28201 gtggcggtca ggtggatatt acatccggca aattcgtttt ctccacttca gaagcgtatc
    28261 tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc gacgttgatt ggttccggta
    28321 tcgaaaccat gcagcagatt tcgatggttg gcaacgacct gaaactggat aacggcgtgg
    28381 gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt gggccagcca acgctgaaag
    28441 ttgataacct gactgttggc ggtactgcgt aataatttca ttattttcag aaggataatt
    28501 aatctttcta cgtgcacgaa cggtcccctc gctcctctgg ggttagggtg aggggaaccc
    28561 gttggcacag gtttgtagaa cgtaacagta caatatgaat tacttctctt tcccgcgccc
    28621 gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg agttgatcac
    28681 gacctgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg
    28741 atctgactga taacgcggca gcaggatctc cagctcaccg cgattgatct cgttgatcac
    28801 ccacatcagc ggcacgtagg cgatcccggc acccgccgtt agccagcgca ccagcgtcat
    28861 cggatcatta gtcacaaatc tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc
    28921 aatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat ggctacttaa
    28981 atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg cgcacaccac
    29041 cattggcatc gcgccaagac ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg
    29101 gatcaccaca tccagaccgt cggcaatcag gtcgggggct ggaattccgg taaccagatt
    29161 gacgctcaaa cctgggtatt ctttcagcat tttggctgtc agcccggcga gaacattttg
    29221 tgccatagtt gaagaacagc caatgcgtag cgtcccgatg ggggtgttat tgaaggcata
    29281 cagttgctca tgaacatcct gcacttcatg aagcatacga cggcagccct ggtagtaaat
    29341 tctaccggct tcggtcaggc caatgctgcg tgtgctacgg tttaacagct ttacctgcaa
    29401 ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct gtagctgtct
    29461 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
    29521 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
    29581 taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
    29641 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctatacaa
    29701 ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
    29761 tattttttga attgctttta tcactggcga ttttctggct ggtgcgccgg gtacttgtgc
    29821 caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
    29881 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
    29941 aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
    30001 aatgcctggg tctattacac cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac
    30061 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcatgataac
    30121 cagctggtga aaaaaggaca gatactgttc accatcgacc agccgcgcta tcaaaaggcg
    30181 cttgaggaag cgcaagccga tgttgcctat tatcaggtac tggcacagga gaaacgccag
    30241 gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag
    30301 gctaacaacg tactacaaac ggttctgcat cagttagcga aagcgcaggc gacccgcgat
    30361 ctggcaaaac tggatcttga acgcacggtg atccgcgcgc cagcagatgg ctgggtgacc
    30421 aacctcaacg tctataccgg tgagtttatt actcgaggat caacggcggt tgcgctggtg
    30481 aaacagaact ccttctatgt actggcctat atggaagaaa ctaagctgga aggggtgcgt
    30541 ccggggtatc gtgcagagat cacgccgctt ggcagtaaca aagtgctgaa agggactgtt
    30601 gatagtgttg ccgctggggt caccaacgcc agcagcacgc gtgacgacaa agggatggcg
    30661 accatcgact ctaaccttga atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt
    30721 ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacagt ggtggtcact
    30781 ggcaaacaag atcgcgacga aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg
    30841 cgtgagtttg gttaatcacg atgggtattt tctccattgc taaccaacat attcgctttg
    30901 cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac ttccagctgg
    30961 aaacgccacg ctgggcggta ctgacagcgg cgattgttgc cgccggtccg gcctttgctg
    31021 cgggaggtga accgtattct ggcgctattc gctatcgtgg ctttttgcgc atcatcggca
    31081 catttattgg ctgtattgcc ggactggtga tcatcattgc gatgatccgc gcaccattat
    31141 tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc tcgctggtac
    31201 gaatagaaaa ctcgtacgcg tgggggctgg ccggttatac cgcgctgatc attgtgatca
    31261 ccattcagcc ggaaccattg cttacgccgc agtttgccgt cgaacgttgt agcgagatcg
    31321 ttatcggtat tgtgtgtgcg attatggcgg atttgctctt ttctccgcga tcgatcaaac
    31381 aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta atgcaactct
    31441 gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca
    31501 ccacggcgct acaaagcatg cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg
    31561 ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc caatcctgcg
    31621 aaacttatct tattcagaat acgcgcccgg aattgatcac tgatactttc cgcgaatttt
    31681 ttgacacgcc ggtagaaacc gcgcaggacg tccacaagca gctcaaacgc ctgcggagag
    31741 ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg
    31801 caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg
    31861 aagaagagat cctgcaaggc gaaccggaag tcaaagtaga gtcagccgaa cgtcatcatg
    31921 cgatggttaa cttctggcga accacacttt cctgcattct gggcacgctt ttctggctgt
    31981 ggacgggctg gacttccggc agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg
    32041 caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctacggg acgctggccg
    32101 cgctgccgtt agggctgctc tactttttgg tgattatccc taatacccaa cagagcatgt
    32161 tgctgctgtg tattagcctg gcagtgctgg gatttttcct cggtatagaa gtacagaaac
    32221 ggcgactggg ctcgatgggg gcgctggcca gcaccataaa tattatcgtg ctggataacc
    32281 cgatgacttt ccatttcagt cagtttctcg acagcgcatt agggcaaatc gtcggctgtg
    32341 tgctcgcgtt caccgttatt ttgcttgtgc gggataaatc gcgcgacagg accggacgtg
    32401 tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat gtggcacgtc
    32461 gtaaagagaa ccacctcccg gcactttatc agcagctgtt tttgctgatg aataagttcc
    32521 caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc
    32581 gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca
    32641 cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac tttggccagt
    32701 tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagcg ccaccgcagg
    32761 tgacggaacc ggttcatcgg ctggcgggga tgctccataa gtatcaacat gcgttgaccg
    32821 atagttaagt caaaaccgac gccaaaagcg tcggtttttt catggctata cttagcgatg
    32881 cacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta tacctttgat
    32941 tttgatgaga ttgagagtca ggaggatttt tatcgtgact ttagccaaac ctttggtctg
    33001 gctaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa cgatgtcctg
    33061 ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg ccgttttggc
    33121 gcgttaatat tgctgtttga tgaggcagag gaagagctgg aagggcattt gcgttttaat
    33181 gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc aatatcgtcg gacaagacga
    33241 tgagggttta tttgtacagt tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag
    33301 taatctggta ggccgttgcg cctttctctt ccgctttttt gttcagcatt tcacgcatat
    33361 ccattggcga agacgccaca ccacttacgg atacggtccc gattgcttca cgattttgtg
    33421 cttgtgcagc atcaatggag tcggcagcga atgcaccgaa agagagaaca gaaagtacgc
    33481 ttaatgcagc tacagtggtt ttgattttca tgatttttac ctcgacataa tcttttagct
    33541 gggcctttgt ttcgtgaccc atatcacaaa atcaagtata cactaattat tgaactaatt
    33601 aataccacgc taactgtttc agttgatact aagtgttaaa aatgtgaatt ttaataacaa
    33661 aaaagtataa aaaacacggt tatttattat taattatagt attttcaatt agataatgaa
    33721 ttttgcataa tgtgcttttt ttaccgtatg tattcatttt gtgaatgatg tgtcgcggta
    33781 aaacgcacta ttcgttaaat gtatcgcagg agaaagaagg ggaatgagag ggataagcaa
    33841 cattttcccc gacgccagaa gcgacggggc agagattaaa gctcctggtc gaacagctct
    33901 aaaattgctt cgtacaggtc tttgacggtg aaaccgttag caggggtggt aaagatggtg
    33961 tcatcgccag cgatggtgcc cagaatacct tctgctttgc ccagtgagtc cagcaggcga
    34021 gcaattaact gcgccgcgcc agggctggta tgaatcacga caactgcatc gttgtagtcg
    34081 atatccagca ccagattctt caatggactg gaggtagttg gtacacccag ttcagctggc
    34141 aggcagtaaa ccatttccat tttggcattg cgtgtacgta cagcaccaaa cttggtcaac
    34201 atccgcgaga ctttagactg attaatattg tcaaagcctt gctcctgcaa cgcggcgacg
    34261 atttcgccct gggagctaaa tttctcttct ttaagtaatg ctttaaatgc tttaactagt
    34321 tcttcttgct tagccgagct tcgcataagt cacccgatat ggtggttgat acaacattat
    34381 tgtgcataca gatgaatttt tatgcaaaca gtcagccctg aagaaggctg aaataatgtt
    34441 atgaaagagc gggattttat caaatttcgt tattgagaaa catgcctgcg tcacggcatg
    34501 caaattctgt ttaaaagtaa attaattgtt atcaaattga tgttgttttg gctgaacggt
    34561 agggtatatt gtcaccacct gttggaatgt tgcgctaatg cataagcgac tgttaattac
    34621 gtaagttagg ttcctgatta cggcaattaa atgcataaac gctaaacttg cgtgactaca
    34681 cattcttgag atgtggtcat tgtaaacggc aattttgtgg attaaggtcg cggcagcgga
    34741 gcaacatatc ttagtttatc aatataataa ggagtttagg atgaaagtcg cagtcctcgg
    34801 cgctgctggc ggtattggcc aggcgcttgc actactgtta aaaacccaac tgccttcagg
    34861 ttcagaactc tctctgtatg atatcgctcc agtgactccc ggtgtggctg tcgatctgag
    34921 ccatatccct actgctgtga aaatcaaagg tttttctggt gaagatgcga ctccggcgct
    34981 ggaaggcgca gatgtcgttc ttatctctgc aggtgtagcg cgtaaaccgg gtatggatcg
    35041 ttccgacctg tttaacgtta acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa
    35101 aacctgcccg aaagcgtgca ttggtattat cactaacccg gttaacacca cagttgcgat
    35161 tgctgctgaa gtgctgaaaa aagccggtgt ttatgacaaa aacaaactgt tcggcgttac
    35221 cacgctggat atcattcgtt ccaacacttt tgttgcggaa ctgaaaggca aacagccagg
    35281 cgaagttgaa gtgccggtta ttggcggtca ctctggtgtt accattctgc cgctgctgtc
    35341 acaggttcct ggcgttagtt ttaccgagca ggaagtggct gatctgacca aacgtatcca
    35401 gaacgcaggt actgaagtgg ttgaagcgaa agccggtggt gggtctgcaa ccctgtctat
    35461 gggccaggca gctgcacgtt ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg
    35521 cgttgtcgaa tgtgcctacg ttgaaggcga cggtcagtac gcccgtttct tctctcaacc
    35581 gctgctgctg ggtaaaaacg gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt
    35641 tgaacagagc gcactggaag gtatgctgga tacgctgaag aaagatatcg ccctgggcga
    35701 agagttcgtt aataagtaat taattagcga ataataaaaa accggagcac agactccggt
    35761 tttttgtttt gagcactcga cttaattggt tgccggatat tcctgaatgg tgacctgcag
    35821 cgttaactgc ttatcatcac gcatcactac aaccgggatc accgaaccag ggcgaatttc
    35881 tgccacctga tccatcgtct ccagagcaga gatggccggt ttgttatcca ccgaaataat
    35941 cagatcgttg acctgaatac ccgcattcgc cgccgggccg tcaggtgaca cttcattaac
    36001 cacgatccct tgcagttgat ctataccacc gccctgcgcg tgcagtggtg cgatctcgcg
    36061 tccgccgata ccaatgtagc cgcggatcac gcgaccatcg cggatcagct tatccataat
    36121 tttggttgct aactggaaag gaatcgcaaa gccgatacct tccggcgttt cgccatcgtt
    36181 actcttatca aacgacagcg tgttaatgcc catcagttct cccagcgagt tcaccagcgc
    36241 gccgccagag ttaccgtggt taatggaagc atcggtttgc aggaagtttt gccgcccggt
    36301 cgggttcaga ccgattcgac ccgtagcact aataatcccc tgggtaatgg tctgcccgag
    36361 gttgtacggg ttaccgatcg ccagtaccac gtcgccaatg tgcggtacgc gacgtgcatt
    36421 gatcggaatg gtaggtaaac cgccagtggc attaatttta agtaccgcca gatcggttag
    36481 agagtcagat cccaccagca atgcttcaaa tacacgtcca tcctgtaagg cgacgatgat
    36541 ctgatcggcg tcgttgatga cgtgtttatt ggtgatgata taaccgcgtt gatccatgat
    36601 tacaccggat cccagggtgc ggatctcaag ctggttgtga gagttggtgt tcaaaccacg
    36661 gttgtaaacg ttaaccaccg ctggcgcggc gcggcgaacc gccagattat aactggcagg
    36721 cgtctcatcg gtactgtcaa attgcggagt ggaaagcggg ttaaggctgc gcagcgaagg
    36781 catggcaacc agcagaatag cgccgacaat taatccaatc gcaacggaac gtaagagctt
    36841 cacaaacatg atggaggcgt cattaaaaaa gggaacggca gcagcatacc acgagttaac
    36901 cggacatcac acgtaagcct gatgcccggt ttacgacatt aacgcatcag cagatagatg
    36961 ctttcattac cgcgtacaat ttgcagggcg atgatggccg gttttgccgc cagcacttta
    37021 cgcatttcag caatcgagtt cacccgatcg cggttgacgc caatgatcac atcgtctttt
    37081 tgcaagccag cctgagcagc tgggcttcct ttgacaactt catcgatctt aatacctttg
    37141 ccgccatctt ttagctgacc atcgctcaac gttgcacctt ccagcgctgg cgtgatcatt
    37201 tcagcgctgg ccgacgaaga ggtgctggta tcaagcgtca cttctacttc cagtggtttg
    37261 ccgttacgca gcaggccaag cttcactttc gtgcccggct ctgtagtcgc gatacgagag
    37321 cgcaactcag caaagctatt cagcggtttg ccgttgaggc tggtaataat atcgcccgct
    37381 ttgacgcccg cttttgccga gccagaacct ggcaacactt cgctgacaaa cgcgccacgc
    37441 tgcacgtcaa ggttgaaggc tttggcgata tcggcactca tctcggtgcc tttgatgcct
    37501 aacaaaccgc gtttgatttc accaaagtcg ataagctgct gcgccagtgt tcgcgccata
    37561 ttactgggga tggcaaatcc aatcccgacg ctcccgccgc caggcgcaag gattgcagtg
    37621 ttgatgccaa ttaactcacc gttaaggttt aatagtgcac cgccggagtt accgcggtta
    37681 atggaagcat ctgtctggat aaagttttcc agaccttcaa gattcaaccc gctgcggcct
    37741 aatgcggaaa caatgccaga ggtggcggtt tgcccaaggc caaatgggtt accgaccgct
    37801 acggcaaaat caccgacgcg caatttatcg gagtcggcaa tagcgatttg cgttaatttg
    37861 ctcgggtttt gaatttgtaa cagggcgata tcgctctggt catcgctacc aatcagtttt
    37921 gcatcaaact cgcgcccatc attgagctga atactgattt tctgtgcctg attaatcaca
    37981 tggttgttgg tcagcacata gcctttactg gcgttgatga tgacaccgga acctaaacct
    38041 tcgaagggtt gtgcaggttg atccggtaaa tcatcaccaa aaaacttttt gaattcttcc
    38101 gggattttct gtccctgact ggccgttcct tccacccgta cgctcaccac tgccggaagc
    38161 actttttcca gcattggagc cagactgggg agaggggcct gatcggcaac ctggcctgga
    38221 atcgacgcga cggcctgaaa tgacgccgag agagttaacc cgacacttaa cgctaatgca
    38281 ctcaacagct gggtttgttt tttcattatt cctgctctcg tacctgaatg ataagaaaag
    38341 agattcaaaa cagtttgatg ttattgaatt ttcagacggc taacaatgag acaccagatt
    38401 aaatgatagt cggggaggga gagaagagga gggcgcaatg gctgcgcccg aaaaataaat
    38461 tagtcgcgct tcgcgccagt acgcagcagg ccggatgcgc cttcagaata gtcgcgaggc
    38521 atctgcaccg gtgcctgatc gttgctggct tcagactctg ccagacgatt acggaacggg
    38581 tttgcttcag cagacagttc cggcagcagg ctgctggagc ttttcgccat gtgctgatac
    38641 agctggcgat agtcgtgcgc catggtatcc agtaattccg cgctgcgggc aaagtggcta
    38701 accagctctt cgcgatactc gtccagttca gctttattct tttccagttc gtactgcaac
    38761 gcctgttgct ggcgtagttt acgattacca aaacgcatgg ccacagcacc aataatgatg
    38821 ccgacgacta acccaattag cgcatattcc caggtcatga acatctcccg ttgtcttttg
    38881 tttccgtagg gtgttggctt caggctccat gcctgcggct gattatgcca ctataaccgt
    38941 taattccaca gaagtggaat cccgactgca tatcgcgtag tgtagaacgg cctttttttc
    39001 gtcaaccgtg aacaacggcg caccgattat tcaaggaata acaataagat catgcaaagc
    39061 gttaccccaa catcgcaata cctgaaggcg ctcaatgaag gcagccatca acccgacgac
    39121 gttcaaaaag aggccgtcag ccgcctggaa attatttatc aagaactcat caatagcagg
    39181 ccaccagccc ccaggacgag tgggctaatg gcgcgggtcg gtaagctgtg gggtaaacgc
    39241 gaagacacaa agcatacgcc agtgcgtggc ttatatatgt ggggcggtgt aggacgcggg
    39301 aaaacctggc tgatggacct tttctatcaa agcctgccgg gagagcggaa acagcgcctg
    39361 cactttcacc gttttatgct gcgggtgcac gaagagctaa ctgccttaca ggggcagacc
    39421 gatccgctgg aaattattgc cgatcgcttt aaagccgaaa ctgacgtgct ctgttttgac
    39481 gaattttttg tttctgatat taccgacgcc atgctacttg gcggtctgat gaaagccctg
    39541 ttcgcccgcg gcattaccct ggtagcgacg tcaaatattc cgccggatga actttatcga
    39601 aatggcctgc aacgtgcgcg ttttctgcct gcaatcgatg ccattaaaca gcattgtgat
    39661 gtaatgaacg tggacgctgg tgttgattat cgactgcgta cactcactca ggcgcatctg
    39721 tggctttcgc cactcaacga tgaaacccgg gcgcagatgg ataaactatg gttggcgctg
    39781 gcgggggcga aacgagaaaa ttcaccgacg ttagaaatca accatcggcc attggcgaca
    39841 atgggcgtcg agaaccagac gctggcggtc tcttttacta cgctgtgcgt cgacgcccgc
    39901 agtcagcatg actatattgc gctctcacgt ctctttcata cggtcatgtt gtttgatgta
    39961 ccagttatga cgcggttgat ggagagcgaa gcgcggcgct ttattgcgct ggtggatgag
    40021 ttttacgagc gccatgtcaa attagtggtg agtgcagaag tgccgctgta tgaaatttat
    40081 cagggcgatc ggctgaagtt tgagttccag cgttgcctgt cacgtctgca agagatgcaa
    40141 agcgaagagt atctgaagcg cgagcatttg gcgggttaaa acctgtcaca aatcacaaaa
    40201 aggggtcgat ctttgacccc gacttctcta taatcctgcg accccacgtt acaagaaagt
    40261 ttttttccca aaactttttg tgtgctggca taggctattc gaaggggtag gtttgccgga
    40321 ctttgtcgtg tgaacctcaa caattgaaga cgtttgggtg ttcaccaacg tgtaactatt
    40381 tattgggtaa gcttttaatg aaaactttta cagctaaacc agaaaccgta aaacgcgact
    40441 ggtatgttgt tgacgcgacc ggtaaaactc tgggccgtct ggctactgaa ctggctcgtc
    40501 gcctgcgcgg taagcacaaa gcggaataca ctccgcacgt agataccggt gattacatca
    40561 tcgttctgaa cgctgacaaa gttgctgtaa ccggcaacaa gcgtactgac aaagtgtact
    40621 atcaccacac cggccacatc ggtggtatca aacaagcgac ctttgaagag atgattgctc
    40681 gccgtcctga gcgtgtgatt gaaatcgcgg ttaaaggcat gttgccaaaa ggcccgctgg
    40741 gtcgtgctat gttccgtaaa ctgaaagttt acgcgggtaa cgagcacaac cacgcggcac
    40801 agcaaccgca agttcttgac atctaatcgg gattataggc aatggctgaa aatcaatact
    40861 acggcactgg tcgccgcaaa agttccgcag ctcgcgtttt catcaaaccg ggcaacggta
    40921 aaatcgtaat caaccaacgt tctctggaac agtacttcgg tcgtgaaact gctcgcatgg
    40981 tagttcgtca gccgctggaa ctggtcgaca tggttgagaa actggacctg tacatcaccg
    41041 ttaaaggtgg tggtatctct ggtcaggctg gtgcgatccg tcacggtatc acccgcgctc
    41101 tgatggaata cgacgagtcc ctgcgttctg aactgcgtaa agctggcttc gttactcgtg
    41161 acgctcgtca ggttgaacgt aagaaagtcg gtctgcgtaa agcacgtcgt cgtccgcagt
    41221 tctccaaacg ttaattggct tctgctccgg cagaaaacaa tttgcgaaaa aacccgcttc
    41281 ggcgggtttt tttatggtta aattctgaat cagcgtaaaa attggaaagt tgctttttgc
    41341 taccgtctga cagacaggca aaacaaaaac cacatcgcca ataagggact aagtcaacta
    41401 tttcagacta aagcgcatct ctttttcccc atttccggca tcgactcacc acaaaggtcg
    41461 caaaatctgg taaactatca tccaattttc tgcccaaatg tcgggtattg ctcatttttt
    41521 gtttgatttt cgaacaaaga gagtcgttcc ttactgggta acacaacttc tgactggcca
    41581 cctggtggct ggtagcagta aaaattctga ctatacctgg aggttttcat ggctgtcgct
    41641 gccaacaaac gttcggtaat gacgctgttt tccggtccta ctgacatcta tagccatcag
    41701 gtccgcattg tgctggctga gaaaggtgta agtttcgaga tcgaacacgt ggaaaaggac
    41761 aatccgcctc aggatctgat tgacctcaac ccgaatcaga gcgttccgac cctggtggat
    41821 cgtgagctga ccctgtggga atctcgcatc attatggaat atctggatga gcgtttcccg
    41881 catccgccac tgatgcctgt ttacccggta gctcgcggtg aaagccgtct gtacatgcat
    41941 cgcatcgaaa aagactggta cacgctgatg aacaccatca tcaacggttc agcttctgaa
    42001 gcagatgccg cacgtaagca actgcgcgaa gaactgctgg cgattgcgcc ggtcttcggt
    42061 cagaagccgt acttcctgag cgatgagttc agcctggtcg attgctatct tgctccgctg
    42121 ctgtggcgtc tgccgcaact gggcatcgag ttcagcggcc cgggtgcgaa agagctgaaa
    42181 ggctatatga cccgcgtctt tgagcgtgac tctttccttg cttctttaac tgaagcagaa
    42241 cgtgaaatgc gtctgggccg gagttaatct gtatggattt gtcacagcta acaccacgtc
    42301 gtccctatct gctgcgtgca ttctatgagt ggttgctgga taaccagctc acgccgcact
    42361 tggtggtgga tgtgacgctc cctggcgtgc aggttcctat ggaatatgcg cgtgacgggc
    42421 aaatcgtact caacattgcg ccgcgtgctg tcggcaatct ggaactggcg aatgatgagg
    42481 tacgctttaa cgcgcgcttt ggcggcattc cgcgtcaggt ttctgtgccg ctggctgccg
    42541 tgctggctat ctacgcccgt gaaaatggcg caggcacgat gtttgagcct gaagctgcct
    42601 acgatgaaga taccagcatc atgaatgatg aagaggcatc ggcagacaac gaaaccgtta
    42661 tgtcggttat tgatggcgac aagccagatc acgatgatga cactcatcct gacgatgaac
    42721 ctccgcagcc accacgcggt ggtcgaccgg cattacgcgt tgtgaagtaa tacaaaaaca
    42781 ggccctggtg gcctgttttg tcttttaatg gcttaataaa atagcgtgat tgtggcaatc
    42841 atattcacca cgaaaccgac tgccatgggg atcgctgttc gcttcaccac cgcaaaaggc
    42901 gaaacgcctg caattcctga cgtaacgacc accaccgcag taatcggcga aaccgcgcgc
    42961 gctagcgtcg tggcaaaatg catcggcata atcattacaa ccgctggtac atgtagtccg
    43021 gctgcgatat tcggaataag actggcaaat gacataaacg gcgcattgcc agagcccatc
    43081 acaatggcac aaatggcaat gactagcgcc ataataatca tcacgccaat accgcccaga
    43141 ccagaatgct ccgcacccct gataaccgca tcaacagtgc caatcgtcgt taagcctttc
    43201 gcaaatattt cacccgcgac caccagcgtt accacgttgg caaactgcgt acccatgccg
    43261 tcaaaaaacg cctgcacatc gtccattgtt tcgcgcaagt tatgcttgcg gaagaactca
    43321 acaaacatcg tcaccgtcaa actcagtaac atcaccgcca ccagatgcag ttctgactgc
    43381 atcagcccga tgtgggcgag gaacagcgag ccgagcatca agattaacgg catcacaggt
    43441 aaaatggcgt aatagagcgg cgggacatta tcgagagctt ttagctctgc ctgttcgtga
    43501 ttgatatttt tatctttttt gtcaaaagcg cgttgcacga aaaagtggga gatcgccacc
    43561 gagataatga cgcaagaggc gaccggaagc tggtagtgga agaagtaagt ggcaattttc
    43621 attcccgcga cctgggctgc aaaaatggag ttcgtttcca gaatccccca ctcaatggac
    43681 atcgtggttg cgataaccgc caccgcagag aggcgactta ctcccagact caccagcgtc
    43741 ggaaataagg tgaccatcag caacataccc aggccggagg cgctggtaat aaactgcgcc
    43801 atgatttggc cgatgacgta agttgccgac agaataatat acggcgagcg aatgagtttt
    43861 aacgggcggc ttagcaagct caccatcgcg cgactggctc ctgtgcgctc catgtagcgg
    43921 gcataaccgc ccactgccat aatcgacagc cccaatccgg caatgcggtt ggacagcatg
    43981 cgcaggattt cattataaat atcgacgatc aagtagccgc tgcttttggc gggatcgagt
    44041 acaccaccga accctaacca gacaccgcac atcatcagga agatacctgc ggcagccagc
    44101 accacctgag gtttgtagtt tttcaggatc aaatagccca tcgtaattaa tacgatgaca
    44161 gatataatta tgccgaacat tgttgaactc cgtgtcaaaa gaaaacggtc aatcccataa
    44221 acggcagatt gaaaacaatg atgttatatt ttttgcaagt ctatttatgg tgcggatgtc
    44281 gtgtttttaa ttgtaggtga ggtgattttt cattaaaaaa tatgcgctta tgattatt
//
LOCUS       DAZKGS010000019        42721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKGS010000019 DAZKGS010000000
VERSION     DAZKGS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            163..1383
                     /gene="ybdN"
                     /locus_tag="ACHOQY_001104"
     CDS             163..1383
                     /gene="ybdN"
                     /locus_tag="ACHOQY_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9677247.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVTLPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1356..1985
                     /gene="ybdM"
                     /locus_tag="ACHOQY_001105"
     CDS             1356..1985
                     /gene="ybdM"
                     /locus_tag="ACHOQY_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9677248.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1986..3146)
                     /gene="ybdL"
                     /locus_tag="ACHOQY_001106"
     CDS             complement(1986..3146)
                     /gene="ybdL"
                     /locus_tag="ACHOQY_001106"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9677249.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3255..4343
                     /gene="hcxA"
                     /locus_tag="ACHOQY_001107"
     CDS             3255..4343
                     /gene="hcxA"
                     /locus_tag="ACHOQY_001107"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9677250.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4353..4550)
                     /gene="ybdD"
                     /locus_tag="ACHOQY_001108"
     CDS             complement(4353..4550)
                     /gene="ybdD"
                     /locus_tag="ACHOQY_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9677251.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4732..6837)
                     /gene="cstA"
                     /locus_tag="ACHOQY_001109"
     CDS             complement(4732..6837)
                     /gene="cstA"
                     /locus_tag="ACHOQY_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9677252.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7018..7431)
                     /gene="entH"
                     /locus_tag="ACHOQY_001110"
     CDS             complement(7018..7431)
                     /gene="entH"
                     /locus_tag="ACHOQY_001110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9677253.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7434..8180)
                     /gene="entA"
                     /locus_tag="ACHOQY_001111"
     CDS             complement(7434..8180)
                     /gene="entA"
                     /locus_tag="ACHOQY_001111"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9677254.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8180..9037)
                     /gene="entB"
                     /locus_tag="ACHOQY_001112"
     CDS             complement(8180..9037)
                     /gene="entB"
                     /locus_tag="ACHOQY_001112"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9677255.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9051..10661)
                     /gene="entE"
                     /locus_tag="ACHOQY_001113"
     CDS             complement(9051..10661)
                     /gene="entE"
                     /locus_tag="ACHOQY_001113"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9677256.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10671..11846)
                     /gene="entC"
                     /locus_tag="ACHOQY_001114"
     CDS             complement(10671..11846)
                     /gene="entC"
                     /locus_tag="ACHOQY_001114"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9677257.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12215..13171
                     /gene="fepB"
                     /locus_tag="ACHOQY_001115"
     CDS             12215..13171
                     /gene="fepB"
                     /locus_tag="ACHOQY_001115"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9677258.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13175..14425)
                     /gene="entS"
                     /locus_tag="ACHOQY_001116"
     CDS             complement(13175..14425)
                     /gene="entS"
                     /locus_tag="ACHOQY_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9677259.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14536..15540
                     /gene="fepD"
                     /locus_tag="ACHOQY_001117"
     CDS             14536..15540
                     /gene="fepD"
                     /locus_tag="ACHOQY_001117"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9677260.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15537..16529
                     /gene="fepG"
                     /locus_tag="ACHOQY_001118"
     CDS             15537..16529
                     /gene="fepG"
                     /locus_tag="ACHOQY_001118"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9677261.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16526..17341
                     /gene="fepC"
                     /locus_tag="ACHOQY_001119"
     CDS             16526..17341
                     /gene="fepC"
                     /locus_tag="ACHOQY_001119"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9677262.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17338..18471)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQY_001120"
     CDS             complement(17338..18471)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOQY_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9677263.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18687..22568)
                     /gene="entF"
                     /locus_tag="ACHOQY_001121"
     CDS             complement(18687..22568)
                     /gene="entF"
                     /locus_tag="ACHOQY_001121"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9677264.1"
                     /translation="MSQHLPLVAAQPGIWVAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22565..22783)
                     /gene="ybdZ"
                     /locus_tag="ACHOQY_001122"
     CDS             complement(22565..22783)
                     /gene="ybdZ"
                     /locus_tag="ACHOQY_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP9677265.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22786..23987)
                     /gene="fes"
                     /locus_tag="ACHOQY_001123"
                     /pseudo
     CDS             complement(22786..23987)
                     /gene="fes"
                     /locus_tag="ACHOQY_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            24230..26470
                     /gene="fepA"
                     /locus_tag="ACHOQY_001124"
     CDS             24230..26470
                     /gene="fepA"
                     /locus_tag="ACHOQY_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP9677266.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26510..26608
                     /locus_tag="ACHOQY_001125"
                     /pseudo
     CDS             26510..26608
                     /locus_tag="ACHOQY_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26645..27265
                     /gene="entD"
                     /locus_tag="ACHOQY_001126"
     CDS             26645..27265
                     /gene="entD"
                     /locus_tag="ACHOQY_001126"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9677267.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27387..27539)
                     /gene="hokE"
                     /locus_tag="ACHOQY_001127"
     CDS             complement(27387..27539)
                     /gene="hokE"
                     /locus_tag="ACHOQY_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9677268.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27890..28042)
                     /gene="hokE"
                     /locus_tag="ACHOQY_001128"
     CDS             complement(27890..28042)
                     /gene="hokE"
                     /locus_tag="ACHOQY_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9677269.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28011..28280
                     /locus_tag="ACHOQY_001129"
     CDS             28011..28280
                     /locus_tag="ACHOQY_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677270.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28484..29602
                     /gene="ybdK"
                     /locus_tag="ACHOQY_001130"
     CDS             28484..29602
                     /gene="ybdK"
                     /locus_tag="ACHOQY_001130"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9677271.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29668..29916
                     /gene="ybdJ"
                     /locus_tag="ACHOQY_001131"
     CDS             29668..29916
                     /gene="ybdJ"
                     /locus_tag="ACHOQY_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9677272.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            29981..30349
                     /gene="ybdF"
                     /locus_tag="ACHOQY_001132"
     CDS             29981..30349
                     /gene="ybdF"
                     /locus_tag="ACHOQY_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9677273.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30443..31096
                     /gene="nfsB"
                     /locus_tag="ACHOQY_001133"
     CDS             30443..31096
                     /gene="nfsB"
                     /locus_tag="ACHOQY_001133"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9677274.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31204..32451
                     /gene="ybdG"
                     /locus_tag="ACHOQY_001134"
     CDS             31204..32451
                     /gene="ybdG"
                     /locus_tag="ACHOQY_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9677275.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32519..33895)
                     /gene="pheP"
                     /locus_tag="ACHOQY_001135"
     CDS             complement(32519..33895)
                     /gene="pheP"
                     /locus_tag="ACHOQY_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9677276.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33997..37140)
                     /gene="cusA"
                     /locus_tag="ACHOQY_001136"
     CDS             complement(33997..37140)
                     /gene="cusA"
                     /locus_tag="ACHOQY_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9677277.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37152..38375)
                     /gene="cusB"
                     /locus_tag="ACHOQY_001137"
     CDS             complement(37152..38375)
                     /gene="cusB"
                     /locus_tag="ACHOQY_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9677278.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQVSQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38391..38723)
                     /gene="cusF"
                     /locus_tag="ACHOQY_001138"
     CDS             complement(38391..38723)
                     /gene="cusF"
                     /locus_tag="ACHOQY_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9677279.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38881..40254)
                     /gene="cusC"
                     /locus_tag="ACHOQY_001139"
     CDS             complement(38881..40254)
                     /gene="cusC"
                     /locus_tag="ACHOQY_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9677280.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            40411..41094
                     /gene="cusR"
                     /locus_tag="ACHOQY_001140"
     CDS             40411..41094
                     /gene="cusR"
                     /locus_tag="ACHOQY_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9677281.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41084..42532
                     /gene="cusS"
                     /locus_tag="ACHOQY_001141"
     CDS             41084..42532
                     /gene="cusS"
                     /locus_tag="ACHOQY_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9677282.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10221 a        11815 c        10914 g         9771 t
ORIGIN      
        1 aaatgagagt ttttgatata taactacaga cagctactat aactttcatc tatttattca
       61 cagcgcgaat gaaaaataaa acactctgct ttgcagtgtt tactttataa ttaacagact
      121 tatctcactg atcaccctgt aacgttcaga gagcgtcttc agatgtctat ttataaaatt
      181 cctcttccgc tcaatattct ggaagctgca cgagagcgta ttacctggac actcaacacc
      241 ctaccccgcg tttgtgtctc attttctggc ggcaaagact ctggtttaat gctgcatctg
      301 acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat cgactgggag
      361 gcgcaattct cttgcaccat taactatgtt cagtccctgc gcgagttgta caccgatgtc
      421 atcgaagagt tttactgggt tacgctcccg cttacgacgc aaaattccct ttcacaatac
      481 caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc cccgcaagat
      541 gcgataaccg atcctgactt tttctgcttt taccagccag gcatgacctt cgaacaattt
      601 gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc
      661 cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt
      721 gccgacgata aaccatggac tacggccgca ccaggcggtc atagctggta catttacccc
      781 atttacgact ggaaagtggc tgatatctgg acctggtatg caaatcatca aaatctctgc
      841 aacccactgt ataacctaat gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc
      901 gaaccttttg gcccggagca acgacaagga ttatggctct atcacgttat cgaaccggat
      961 cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga
     1021 catgataatc atttctatgg gcatcggaaa atcctcaagc cagaacattt agactggcaa
     1081 gaatatgcat tattgctgct caatagcatg ccggaaaaaa cagctgagca ttaccgcaat
     1141 aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc acaaacccag
     1201 caaggggaca ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa agttttactc
     1261 aataacgatt actggtgtcg ggcattatca tttagtccta cgaaagcgaa gaactatcag
     1321 cgttataacg aacggataaa aggaaaacgt caggaatggg ggatactatg caacaacgat
     1381 taacgcagga tttaacgcaa tttctcgcca gcttgccgga agatgatcgc atcaaagcga
     1441 ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt
     1501 gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg
     1561 caccacccga aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt acccaaccca
     1621 ttgtggtcac gcatacggat aaaaatgccc tggaaatcgt ggatggtttt caccgccatg
     1681 aaattggtaa aggctcatca tcattaaaat tacgcttaaa aggttatttg ccggtgacct
     1741 gtctggaagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg
     1801 gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga acttagccag ttgggatggg
     1861 acgataataa aattggcaaa gagctaggaa tggacagcga cgaagtgctg cgcctgaagc
     1921 aaattaacgg cctgcaagag ctgtttgccg accgtcaata ttcccgcgcc tggacggtta
     1981 aataactaaa gctggcgcag gcgttcagct gctgccagca acgtcgattc cttcttggca
     2041 aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga cagcggaatc
     2101 gccgctacgc cgtgctcctg cgtcagccac tggcaaaact caacatcatc cagggtagaa
     2161 acctcgctgt aatccaccag caaaaagtat gtaccttcac acggtaaaat ctccagccgg
     2221 ctttcattta aagcattcac cagaatatcg cgcttctggc gataaaagtc cggtaacgca
     2281 agataatgct caggttctgc acgtagcata tcagcaagcg ccagctgtgc cggggtattc
     2341 accgaaaagg tcagatactg atgtaccttg cgaatttcgg cgctgatggg cgctggcgca
     2401 acacaataac ccactttcca gccggtcata tgataggtct tgccaaatga agaaaccgcc
     2461 actgcccgct cacgcagctg cggatgcgcc agcacactgg catggccctg ttgtgaaaag
     2521 ttgatgtgct cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc
     2581 cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tggggttatg cggagtgttg
     2641 aggatcacca gtctggtgcg ctcgcttaat aatgcggcaa attcctgcca gtcaacgcga
     2701 aaatgcggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg
     2761 gagtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg
     2821 gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca
     2881 tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc
     2941 acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc
     3001 tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt
     3061 tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt
     3121 tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg
     3181 cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat
     3241 aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact
     3301 tttcacatcc aggaagtttc aatcacctgc acgatttttt cactgatgaa caactttctc
     3361 gcgcggtgtg gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag
     3421 cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg
     3481 tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg
     3541 gcgcactgct cgacaccgcg aaagccctcg cccgccgtct cggtctgccg tttgttgccg
     3601 ttccgacgat cgccgccacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg
     3661 ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac
     3721 cggagattat cctcaatgca ccgcaacaat atctgctggc ggggatcggt gacacgctgg
     3781 cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc
     3841 gactggggat caataatgcg caggccattc gcgacgtatt gttaaacagt agcgaacagg
     3901 cgctgagcga tcagcaaaat caacagttaa cgcaatcatt ttgcgatgtg gtggatgcta
     3961 ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg
     4021 ctcatgccgt gcataacggt ctgactgtcc tgccgcaaac cgagaaattt ctccacggca
     4081 ccaaagtcgc ttatggcatt ctggtacaaa gcgccttgct gggtcaggat gatgtgctgg
     4141 cgcaattaac cggggcgtat cagcgttttc atctgccgac cacgctggcg gagctggaag
     4201 tggatatcaa taatcatgcg gagatcgaca aagtgattac ccacaccctg cgtccggtgg
     4261 aatccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa
     4321 aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca
     4381 ccgtagcgcg cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg cgtttgatcg
     4441 ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc
     4501 ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc
     4561 gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     4621 tgctatcaac gcgctcggtc agtcccctcg ccccatcggg gagagggtta gggtgaggag
     4681 gaaccctcgg cactatgcca acattcgcca acatcccccc tcactctgac tttagtgtgc
     4741 gccttttgcc tgtgccacga tctcctcgac attttccggc attggctcat acggcgtttc
     4801 tttcgccgtt ggcttcggat ctttcaatgc cgccagtgcc gtcttaatcg agaacagcgc
     4861 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
     4921 caccagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat
     4981 tgcctggaac ttattagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt
     5041 ctgccagcct gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca
     5101 ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc
     5161 tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatgc cgcccagcgg
     5221 atcgaccacg ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ctgttgccag
     5281 caggttagca ggcagtgaat cggtccgctt caggcccgga gaaaccacgc ccagcagatc
     5341 ctgcaacata aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc
     5401 ttcaaacaga atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc
     5461 gtgcaggatg taagccatcc ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat
     5521 cgactgttca cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc
     5581 ccagctactc accacctgcg cggcagaagc gactacatcc gccgtccctg ccggagccag
     5641 caccgccatt gggctgttca tggcaaaata cacgcccgga tcgatgatac aggcagaaac
     5701 cagcgccata atcgccacga aggattccat caacatccca ccgtagccga taaagcacgc
     5761 ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc
     5821 agacaccgca ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac
     5881 cgggccagtg ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat
     5941 caaaatgcct accgccagac cgacgatagt cccgattttc aggaaggtcg agagatagtc
     6001 acgcggggcc agcagcaacc acaccggcag caccgccgcc acaaaaccgt agcccaccag
     6061 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
     6121 cacccagccg ccagagataa tggcgaaaat caggaacacc aggccaatca ctgacacttc
     6181 gccaatgcgg cccggacgca gatagcgcag gtagattccc ataaaaatcg ccagcggaat
     6241 ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat
     6301 cgccagtact gcaaggataa tgaccatgat cataaagcag gctaccagcg caatcacccc
     6361 ggcagttggc cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt
     6421 agaaacaaac agcaccatga aatcctgcac cgcaccagcg agaaccaccc cggcgagcag
     6481 ccagatcatc cctggcaggt aacccatttg cgccgccagt accggcccaa ccaacggacc
     6541 tgctccggca atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
     6601 gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa ccgccagcac
     6661 atttttggcg atatacagcc cataaaaacg gtaagcgatc agatagatac agaccgacgc
     6721 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc
     6781 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
     6841 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtaag
     6901 atttaacaac acggtaacca tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
     6961 taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca
     7021 tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac
     7081 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac
     7141 cttgccttca gacaccgggc gatgatgggt ggcgttaagt tccgtaccca ccacacactg
     7201 cccgtcacgg gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga
     7261 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc
     7321 ttccagcaca tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt
     7381 atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
     7441 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca
     7501 gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga
     7561 atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc
     7621 acccacagcg tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc
     7681 acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc
     7741 gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg
     7801 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac
     7861 gcaccgccga cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc
     7921 gcgcccatgc gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct
     7981 aacagtcgct gacatacctg cgcgacctgc gcagcatcgg caacatccat cacttcggtc
     8041 gcaaagggat attgctcctg agcgaacgct tgatcaaaac cggtaacttt cgctcccgcc
     8101 tcaacaaacg ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca
     8161 tttttaccgc tgaaatccat tatttcacct cgcgggagag tagcttccac caggcgtcga
     8221 tggtcgggtt tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg
     8281 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
     8341 acggttcatc ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg
     8401 cggggacagg tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca
     8461 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca
     8521 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
     8581 catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg
     8641 gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
     8701 tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat
     8761 cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaaccg
     8821 gaatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca
     8881 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
     8941 acgcggcacg ttgcggttca aaggcccagt caactttatt cttagggata tcgtgagact
     9001 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
     9061 gcgcgtgacg ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc
     9121 ggaagtgaat ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
     9181 cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcgcag
     9241 cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcgtagat caccgccggg
     9301 tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcaccgcc acggttgatc
     9361 tgatctttct cgcgcccctg cacggtgatg tacccctctg gatcaataga gatcagatcg
     9421 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg gcttttgtaa
     9481 tagccacgga aggtgtacgg accgcgggtc atcaggcgtc caacttcccc ttgcggcagt
     9541 ggatttcctt cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg
     9601 gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc
     9661 atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg
     9721 gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt
     9781 tcaccttcgg tcagcgcctg caaccacagg ctgactgccg gtggcaccag cgcggtgacg
     9841 ttcacctgat gtttttcaat caatgggaag caaagcgtgg cgctgggatc ggccgccaga
     9901 acaaccgttc ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagtta
     9961 tgagccgccg ggatcgcgca caggtagcgt gtctgttgtg tgaactgaca aatctcgacg
    10021 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt cggtgtgccg
    10081 gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgatgg cgtggcagta
    10141 aaatcctcag ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc
    10201 agttgcacca cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg
    10261 ctaaacagcg catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag
    10321 gcgttcagtt cactacgctg atggctgaac agcgccagca ccggcgcaac gcccagtttc
    10381 agcagcgcga aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt
    10441 tcaccaggtt taatgccctg acggcgtaaa ctacacgcga ggttatccgc cgcctgattc
    10501 agctcccggt aactcaactg tcgctcgccg tcgataaccg cgatgctgtc actcgcagcg
    10561 tggcgagtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga
    10621 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat
    10681 ccaaaaacgt tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt
    10741 gacgacgcag gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    10801 gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg
    10861 ccgccaaaca gttcgcggtc gaatggctcc agttcagcaa taacctgggt cgcggcctga
    10921 tgcgggaaac cgctcagcgc gggggttgga tgcagcagac aggccagagt cagtgcgttt
    10981 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta
    11041 atcaattgtg gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc
    11101 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    11161 cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg
    11221 gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg
    11281 ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    11341 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa tcgtgacaac
    11401 accactttgt cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac catctgttca
    11461 aacgtggttt gttcaggaat tgcctggcgt tccaccacgt tcagcgactg gctgcgggta
    11521 aaacggcggg ctgaggtctg tttttcctgg cgggagaacg actgccagga ttcgggaata
    11581 tacagcgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg
    11641 atgccctgcg ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    11701 ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    11761 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    11821 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    11881 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    11941 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga
    12001 agcattattg atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc tgccccctct
    12061 cccttccagg gtgagggctg gggtgagggt taatgttcgc accagtgctg gctgttcccc
    12121 tcaccctctc cccaaagggg cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac
    12181 gcccataacc ttattaataa caggaagctg atttgtgaga ctcgccccgc tctaccgcaa
    12241 cgcccttcta ttaacaggac ttttgctttc aggaatagcc gcagttcagg ccgctgactg
    12301 gccgcgtcag attactgaca gccgtggcac acatacactg gaaagccagc cgcagcgtat
    12361 tgtttccacc agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc
    12421 cagcggcgcg accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg
    12481 gagcaaggtg gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcaccga
    12541 agccgttgcc gcgcaaatgc cggatctgat tttaattagc gcaaccggcg gggattcggc
    12601 gctggcactg tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga
    12661 caaaagctgg cagtcgctgt taacgcagct tggcgaaatt accgggcatg agaaacaagc
    12721 ggcagagcgg attgcacagt ttgataagca actggcagcg gcgaaagagc aaatcaaatt
    12781 accgccgcag ccggtcactg ccattgtcta taccgccgct gcacacagtg ccaatctctg
    12841 gacgccggaa tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt
    12901 gcccgcaggg ttaaacgcca gccaaagcca gggcaaacgc catgacatca ttcagcttgg
    12961 tggggaaaat ctggctgctg ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca
    13021 gaaagatgcc gatgcgattt atgccaatcc actgctcgcg caccttcccg cagtacaaaa
    13081 caagcaggtt tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccacgca
    13141 agtgctggaa cgacttaagg cgctgtttta agcattaact gtcagacgct gtcacctgcg
    13201 gcggcgtctg gcgaaaacgt cgcaactcca ccagcaccag caataacaac acgccgataa
    13261 tcaacaaacc aaaaccgctt gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac
    13321 cacccagcag cgccgcgcct atggcatcgc ccgtcacgtt ctgcgccgtc cacaaaccgt
    13381 taatccgccc taacatcgct tccggggttt gcgtttgcag cattgtatat tgcagcaacg
    13441 aactaaccgc gctcagccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg
    13501 gcatcaggcc aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata
    13561 accctggtcg cgcactatgt gccagcttcc cgctggttaa cgcaccaata gccgcgccga
    13621 gcgggatcgc cgcgtagaga aaaccaatct gtgccgctga catctgccag ttgtcagcca
    13681 gcgccggata cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc agcagcgcaa
    13741 tcccgcccac cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg
    13801 gatgctcacg cggctgcggt ggcggtggca acgccggaag gcttaacaac ggtagcaagg
    13861 taataaacgt gcccgccgcc gccagcccgt agttccaggc tacgccaccg gtcgccagca
    13921 ataaaccgcc aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg
    13981 ccccggcctg cattaagttt tcacgcccta ccagtgctgg tgtcgccgcc aatagcgccg
    14041 taacgccaag cgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg
    14101 acggctccgg cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc
    14161 gcgccagcaa aatcactttt ttgcgctcat agcgatccgc cagcacaccg ccaaccatca
    14221 ggccaacaaa catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat
    14281 gcgtcatcat ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg
    14341 agataaaacg agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt
    14401 taagcagcca ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta
    14461 ttcttaggct gcacatgcta acatatccaa ataagataga taacgataat taatttcatt
    14521 atcatggaag ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga
    14581 ttgctgttat tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc
    14641 cccgcttccg tagtgctgga ggccctctcc ggcacctgcc agagcgccga ctgcaccatc
    14701 gtgctcgacg cgcgactgcc gcgcaccctt gccggtttac ttgcaggcgg cgcgcttggc
    14761 cttgccgggg cgttgatgca aaccctcacc cgaaatccac ttgccgaccc cggcttgctt
    14821 ggcgtgaatg ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca
    14881 tccgcgcagg aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt
    14941 gcctttaccg gcagtcaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc
    15001 gtggcgctgg cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgat
    15061 gtctacgatc aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc
    15121 ttaaaagtgg tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt actgagccgc
    15181 gcgctgaaca gtttaagcct cggcagcgac accgcgacgg cgctgggcag tcgcgtggcg
    15241 cgcacacagt taattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta
    15301 gttggcccga ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt
    15361 gccgatcatc gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt
    15421 gccgatatca tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg
    15481 tttattggtg caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggcgcatga
    15541 tttacgtctc tcgccgatta atcatcacct gtttgctgtt ggtttccgcc tgtgtggttg
    15601 caggtatctg gggattacgc agcggtgccg tcacgctgga aacctcgcag gtattcgccg
    15661 cgctgatggg cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac
    15721 gcgtgctgat ggcgctgttg attggcgcgg cactgggcgt cagcggcgcg atttttcagt
    15781 cgctgatgcg taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga
    15841 gcggcgtgct ggtagcgatg gtgctgtttg gtcaggacct gacggctatc gcgctggcag
    15901 caatggtggg cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggca
    15961 tcgacacctt tcggttgatt attatcggta tcggcgttcg tgccatgctg gtggccttta
    16021 atacctggct gttgctgaaa gcgtctttag aaacggcgct aacagcgggt ttgtggaatg
    16081 caggatcgct caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat
    16141 tgatgctcat tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg
    16201 ataccgcgtg tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg
    16261 cagtggtgct taccgctgcg gcaaccgcgc ttgccgggcc gatttccttt attgctttag
    16321 tcgcgccgca cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg
    16381 cgctgtgcgg ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc
    16441 cgtatcaact tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct
    16501 tgttaattca ggagtctcgc aaaaaatgaa cgaatctgta gcccgtttgc gcggcgaaca
    16561 gttaaccctc ggatatggca aatataccgt tgcggaaaat ctgactgtag aaatacctga
    16621 tggtcacttc acggcaatta tcgggccaaa tggctgcggt aaatccactt tactgcgtac
    16681 tttaagccgc ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca
    16741 acattacgcc agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa atgctaccac
    16801 gccaggcgat atcaccgtgc aggagctggt ggcgcgtggg cgttacccgc atcaaccgct
    16861 atttacccgc tggcgcaaag aagatgaaga agcggtaacg aaagcgatgc aggccacggg
    16921 aataactcat ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgcgc
    16981 gtggatcgcg atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac
    17041 ctggctggat atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga
    17101 gaaaggctat accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag
    17161 ccatttgatt gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt
    17221 cactgctgaa ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt
    17281 agccggaacg ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta
    17341 aactaagtgg tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa
    17401 cacgctacca caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt
    17461 acccggaccg tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa
    17521 attcacatcg ttgatatttg cttttgttaa ctgctcaaca agatactgac gattacgtaa
    17581 ttcaccgttc agttccgcaa cgtcagtgac cgctttttca atttccagtt tgcgttcaat
    17641 accgtctgcg ccgagagaaa tagagaaatc ggggtcatct ttaacggcct gaccattact
    17701 gtatacgggc tttttaattc ccgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg
    17761 aatgtttgca tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc
    17821 aaactgggtt ttgatttcca gtttattacg gacgttttct agtgactctt tcaccaccaa
    17881 cgcagagata taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc
    17941 ggtaaaactt agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt
    18001 gtcatcaacc gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc
    18061 atcgattttc gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc
    18121 cagcaagcta accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat
    18181 tttgatatcc agatccagca ctcgaagctt agtgaatgtt ttctccagct cttgccactg
    18241 aacaggttct ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga
    18301 gatcagcaag cctgcgcagg caaacgcaaa aatgaccgcc atgaccgttt ttttggcccg
    18361 ccataaaact gcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg
    18421 atactcagga aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa
    18481 cctgaaaatg agtccaatag gggaaatctc cacggcgctc actttaccag ttggtgataa
    18541 ttatccgaag ctgaagtttg taaattcctt ccactgaatg caacattcac tctatttaag
    18601 agaaatattc atcaaaccta taaatcaaac aaataaaaaa acccataatt attttctgta
    18661 attatgggtt tatagatatt attaatttac ctgtttagcg ttgcgcgaat aatcggccca
    18721 atttttacaa acgcccctgg agagataata tccacatgcg cacaatcctg acgatagata
    18781 tccagctccg caatccacgg cgaccaggca cgttcgggac tcataccttc ctgaagtgtg
    18841 cgctcagcaa caaataacgt tgcttttcca tcaaacggaa cgctatgggc ggtggttaac
    18901 aagcgcacag catcagcgta gttgccttca atggtggtaa acaactccgt tgaagtactt
    18961 ccctgctgtg ccgccaggaa ggcctcgcgt tcgcggttaa tctccgccag cacttccggg
    19021 tccagaccat tagcctcttt ttcctgccag ttttgcgtct ctggcggcca ggtatccagc
    19081 aaaccaagaa atgccacctg ttcgccacgg gcacgcagtc gcgccgcaat accctgcgcc
    19141 agcgtaccgc caagggaata ccccagcagg taataagggc cgtgcggttg ttgctcaagt
    19201 aacgttgcca gatgcgcttc gcagacttca tccaggtttg tcgctgtctg catgggtcca
    19261 tgagggcgcg gcgactgaat gccaataatc gaccattgtg gatcgagata acgcgagagc
    19321 acgctaaact gccaggcaaa accggacgcc ggatggaaac aaaacagcgt cgggccatta
    19381 ccttcacgca acggcagaat ggtttcgaat cccatgcgcc gggagctgtc ctcttcacca
    19441 tcaataatcg ttgccagttt ggcgacggtt gacgcgacca tcacctgccc cggcgtcacc
    19501 tgacgagcaa actgtcgact taactgcgct gctagtttca ttgccagtag cgaatgaccg
    19561 cccagcgcaa agaaatcagc atcggcatcc tgcacgtcac aacccagcaa cgacgcgaat
    19621 gccgcggcga taatcgtttc actgcccgct ttcggtgcac gtcccgacgc ttgtgtcttc
    19681 agttcaggca acggtaaggc tttgcgatcc agcttgccgt tggcgctaag cggcaactgc
    19741 ggaagttgca gcagaacgac cggcaccata tgcggcggca atgtttcgcg aagctgtgcc
    19801 tgtaatgcgc tggtatccaa cggcagtccc gattgcgaca ccagatagcc caccaactga
    19861 cgcgcatcac caccggtggc tgccgcctgg ttaatcacac aggcgtgggt aactgcctgt
    19921 tcgacatccg gcagcgcctg catcacgcga tcgatttcgc ccagttcgat acgctgcccg
    19981 cgaattttta gctgatcgtc actgcgcccg aggtactcca ccgcgccgtt atccagccag
    20041 cgggcgacgt ctccggtacg atacatccgt tcacctgggg caaaaggatc ggcaataaaa
    20101 cggctggcgg tcagatcggg tcgtccaaga tacccctgcg ccagttggat gccggtgaga
    20161 tagagatcac ccgccacacc cggcggcacc ggatgcatca tcgcatcaag aatacgcaga
    20221 cccgtattcc ataccggata accaatcggc acactgctgc cgcgcacctg tgccagttcc
    20281 tcgccaaaag ccggatacca gctgacatct accgccgctt ccgtcgggcc atacaggtta
    20341 tgcaacggcg cgccggttaa ctgttgccat tcgcggcata aatcagccgg taaggcctca
    20401 ccactacaga aaacctgttt caacgtcgcg caattctggc gagcggtttg cggcgtcagc
    20461 gatgcaacaa atgccgccag catcgacggc acaaagtgcg tggtcgttac gccatattcg
    20521 gcaaagaatt gctgcatagc gagcgggtca cggtgcgctt ccggttcagc catcaccagt
    20581 tttgcccctg cgataaacgg ccagaaaaac tcccacaccg agacatcaaa actgcacggc
    20641 gttttttggg caacgacatc ttcgcctgta agcgggtagt gattttgcat ccataacagg
    20701 cggttaacga tagccgtctg cccgaccatt acccctttcg gcctgccggt ggagccagag
    20761 gtaaagatga tataagccgt gtggtgcggt tgtgaaagtt gcagcggcgc actgccctgg
    20821 ggggtaagcg gggcgttata gcaaaggctt gttaaattgg gaacatcgct aaagcgcggc
    20881 agttgatcgt cggtggtaat taacagcgac ggacgcgcat cttccagcat cattttcagg
    20941 cgatcgtccg gatagccggt atccagcggt agccaggccg cgccagcttc aactattgca
    21001 tggagtgcca gggtcaaaaa gaccgagcgc ggtagtgcca ccgccacgct gtcccctggt
    21061 ttaacgccgc gctcacgcag cagattcgcc agcgccacca cctgctcgcg catttcccga
    21121 tagctgaaca ggtaacgcgc atctgccagc gccggagcat ccggtgtttt tgccgcttgt
    21181 tctgccacca gcgcgctcag cgtggtttct ggaatctcaa cctgagtggc gttgatctgc
    21241 gccagctgcg catactcacc tggcagcata atatcgacat cgccgcacaa cagcgccgga
    21301 tccgcggcga actgggcaat cagcattttc aggcgttcag catgctggat taacgttggc
    21361 tcatcgtaac gctgtttatt ggcgaggatc tcaatactca aatcaccgtg ttcatccggg
    21421 aacagggcca gttcaaggtc attaaccgga ccggttgcca gggtatgggt ttgcgtctga
    21481 acaccaggaa tatccagttg gtaatcaaat accttgatat tgagtaccgg accgaacagc
    21541 ggttcctctc ctgccgctcg cccgctgtca cggacaattt gttcggcatc gtaacgttga
    21601 tgacgacgca tttttttcag ttgtgctgcc agtcgggttg ccagttccgg cagcgtttct
    21661 tgcgccgcaa tgtgaatacc caacggtaaa acgttgagca cgggtccgct agccgtcagc
    21721 gccgccgagc ccagtcgacg cataaagata aatccggcgg catagtccat gcgattgcac
    21781 aatcgcccca gccacaaggc tgccagcgca agggctaaat cggtacgctg cacacctgaa
    21841 agttgcgtag ccagctggcg gaattccccg tcggtaaatt ccagtttcag gcgcagaata
    21901 tctgccgaag cgctgcgccc cgctaaaggt gccggagaaa gtgacgcggg cggcggcagt
    21961 tgacgacgct gttctgccca gaatgccgca tcacgctgcc aggcttcgct ttcgcggtat
    22021 tgctggtact cttccactac atcagcgaaa ggcgtaaacg gcgaagcagg cgttggttcg
    22081 ccacgcagcc atgtgcagta aatattggcg atctggcgag taatggccgg gaaactgaag
    22141 ccatcgacca gcaaatggtg ataacgctga taccagtacc agcggttatc cgcaacatgt
    22201 atcagctgat gaaagaccag tggtttaccg ctatcgacgc gcaaatcttg ttgcaaatcc
    22261 gccagcatta atgcctgcgc agtaccgtgc ggatcaatat tggttcgcag gtcgataatt
    22321 tctggcagtt cgaatatcag cgcatcatcg acccactgcc agacttcgcc gttatcttcc
    22381 gtaaaacgca tccgcaacgt atctgcttgc gctagtccga caaccaccgc gcgggccagt
    22441 aatggcgcat caacatctcc agttaactca acgtaatgcg ccacgctcca ggcggagggt
    22501 aaatccgaca gtttttctgc cacccagatg ccgggctgtg cggcgaccaa aggtaaatgc
    22561 tggctcattg tgcctcctgc aactgggtga aattggccgg tgtcagagta cgccagtgga
    22621 cttccagcca ctgctggcag gacgcctgtg actgcggctg acacacaatg tcccagcctg
    22681 ccggtaaggc gcattgttgc ggccacagac tgaattgccc ctgcgcattg cgcaaaatgt
    22741 aaaacgctcc ctgtggatca tcgaagggat tactgaatgc catattcaac tcctgtcatg
    22801 gaaagtggtt gccagaggtc gattagcccc tgcatcaagc caccgcgcca acaaagcgca
    22861 tcatgtccgc cgtcaacctg acgccagaaa atggattctt ttatggggtg taattgcgca
    22921 tacagcgcct gattggcccg cataatcatc ggctcgcgaa tacccgcttc cagcacaatg
    22981 cgcagacctt cggcgctaac ttcaccagct ttaagctttt caagtaacac gccctcttgc
    23041 tgcccgcccc gatgcggcca ccagtacgat cctgactggc ttaatacaca gccaaagcgt
    23101 tcaggccagt gcagtccggc atacagcgcg gacagcccac caaaactctg cccggcgacc
    23161 acggtgcgat cggcgcgatc gctaaaaggg gcaatagctt tcaccagggg taataactct
    23221 tgctgtactg ccagccagaa atccgcatta cacggcagtt cgtgggcgcg gtgcgtggtg
    23281 tcgatagcgt cgatcaacac atacacggcg ggaggaagtt gctgacgatg ggtcagcgaa
    23341 gtcagcacag gccagacggg catactttgc gcccaaaatt cgccatcgag caaaactgcc
    23401 agcggacgtt cttctgctgt tgcatcgccg gtggtaaaaa tccatacacg acgtgaattt
    23461 ttcaaccgtt cacttttcca gataatttct ttggctgcta tttctggcgc ttgcggacaa
    23521 tcccatcccg gttgcagagg cgcttgcggc atttcgagtg cagaaacagc gtgccctcgc
    23581 ccgcctttcc agctttgtgg attcagcgga tcggctattg cctggggtaa tagttttcgc
    23641 cagccttcac gtaattcgag gcgatcgggg gatggtgcag aaaaaatgtc atcgcgttcg
    23701 gtgggaataa agcagtagct gccgcgccag ttggcattga gttgtgtcgt ccactgccag
    23761 acatcagtac ctgcaattcg ctgcatcgac tggggctggc tgttctgatg atgatcggtc
    23821 acaccagtga tgtagatcca tacgcgcttt atcgtcgagt attcttcaga accttgggga
    23881 tcacgccacc agaaagtgac ctcaaacatt tcgtcattca gacgctgcca ttccgggcca
    23941 tgtttcgact gccaccagct ctcacttcct acttttaacg ccgtcacacc ataaccccat
    24001 gtttactgtg caatttttca ttgattgcag aaatatattg ataatattat tgataactat
    24061 ttgcatttgc aatagcgtaa tggcgcgccg tgggaagcgc ggacatttat taaccaactg
    24121 cactgcgtgt ctttcaggat caaaggtttt cgcggtagcg ggatgcgtcg tgttgaggac
    24181 gaccatgccc gacagttgca attcgtggca aaaatgcagg aataaaacaa tgaacaagaa
    24241 gattcattcc ctggccttgt tggtcaatct ggggatttat ggggtagcgc aggcacaaga
    24301 gccgaccgat actcctgttt cacatgacga taccattgtc gttaccgccg ccgagcagaa
    24361 cttacaggcg cctggcgttt cgaccatcac cgcagatgaa atccgcaaaa accccgttgc
    24421 ccgcgatgtg tcggagatca tccgtaccat gccaggcgtt aacctgaccg gtaactccac
    24481 cagtggtcag cgtgggaata accgacagat tgatattcgc ggtatgggtc cggaaaacac
    24541 gctgattttg attgacggca agccggtaag cagccgtaac tcggtgcgtc agggctggcg
    24601 tggcgagcgc gatacccgtg gtgatacctc ctgggtgcca cctgaaatga ttgaacgtat
    24661 tgaagttctg cgtggtccgg cagctgcgcg ttatggcaac ggcgcggcgg gcggcgtggt
    24721 taacatcatt accaaaaaag gcagcggtga gtggcacggc tcctgggacg cttatttcaa
    24781 tgcaccagaa cataaagagg aaggagccac taaacgcacc aacttcagcc tgaccggtcc
    24841 gctgggcgac gaattcagct tccgcttgta tggcaacctc gacaaaaccc aggctgacgc
    24901 gtgggatatc aaccagggcc atcagtccgc gcgtgccgga acgtatgcca cgacgttacc
    24961 agccgggcgc gaaggggtga tcaataaaga tattaacggc gtggtgcgct gggacttcgc
    25021 accattgcaa tcgctggaac tggaagcggg ttacagccgc cagggtaacc tgtatgcggg
    25081 cgatacccag aacaccaact ctgacgctta cactcgctcg aaatatggcg atgaaaccaa
    25141 ccgcctgtat cgccagaact acgcgctgac ctggaacggt ggctgggata acggcgtgac
    25201 caccagcaac tgggtgcagt atgaacacac ccgtaactcg cgtattccgg aaggtctggc
    25261 gggcggtacc gaagggaaat ttaacgaaaa agcgacacag gatttcgtcg atatcgatct
    25321 cgatgacgtg atgctgcaca gcgaagttaa cctgccgatt gatatcctcg ttaaccagac
    25381 gctgacgctg ggtacggagt ggaatcagca acggatgaag gacttgagtt ccaataccca
    25441 ggcgctgacc ggggcgaaca ccggcggcgc tattgatggt atgagtgcca ccgaccgtag
    25501 cccgtattca aaagcagaaa ttttctcgct gtttgccgaa aacaacatgg agctgactga
    25561 cagcaccatc gtaacgccgg ggctgcgttt cgatcatcac agtattgtcg gcaataactg
    25621 gagcccggcg ctgaacatat cgcaaggttt aggcgatgac ttcacgctca aaatgggcat
    25681 tgcccgtgct tataaagcac cgagcctgta ccagactaac ccgaactaca ttctctacag
    25741 taaaggtcag ggctgctatg ccagcgcggg cggctgctat ctgcaaggta acgatgacct
    25801 gaaagcagaa accagcatca acaaagagat tggtctggag ttcaaacgcg acggctggct
    25861 ggcgggcgtc acctggttcc gtaacgatta tcgcaataag attgaagcgg gctatgtggc
    25921 tgtagggcaa aacgcagtcg gcaccgatct ctatcagtgg gataacgtgc cgaaagcagt
    25981 ggttgaaggt ctggaaggat cgttaaacgt accagttagc gaaacggtga tgtggaccaa
    26041 taacatcact tatatgctga agagtgaaaa caaaaccacg ggcgaccgtt tgtcgatcat
    26101 cccggagtac acgttgaact caacgctgag ctggcaggcg cgggaagatt tgtcgatgca
    26161 aacgaccttc acctggtacg gtaagcagca gccgaaaaaa tacaactaca aaggtcagcc
    26221 agcggttggc ccggaaacca aagaaatcag tccttacagc attgtgggtc tgagcgcgac
    26281 ctgggatgtg acgaagaatg tcagtctgac cggcggcgtg gacaacctgt tcgacaaacg
    26341 tttgtggcgt gcgggtaatg cccagaccac gggcgatctg gcaggggcca actatatcgc
    26401 gggtgccggg gcgtatacct ataacgagcc gggacgtacg tggtacatga gcgtaaacac
    26461 tcacttctga tgctaacgtc agattgttga caaagtgcgc gtcgttcatg ccggatgcgg
    26521 cgtgaacgcc ttatccggcc tacaaaaatc ttgccaattc aatatattgc aggaccatgt
    26581 aggcctgata agcgtagcgc atcaggcagt ttagcgtttg tcatcagtct ctaatatggt
    26641 cgacatgaaa actacgcata cctccctccc ctttgccgga catacgctgc attttgttga
    26701 gttcgatccg gcgaattttt gtgagcagga tttactctgg ctgccgcatt acgcgcaact
    26761 gcaacacgct ggacggaagc gcaaagccga acatttagcc gggcggatcg ctgcaattta
    26821 tgcgctgcgg gaatatggct ataaatgtgt gcccgcaatc ggcgagctac gccaaccggt
    26881 gtggcctgcg gaggtatacg gcagtattag ccactgcggg gctacggcat tggccgtggt
    26941 ttcccgtcaa ccgattggcg ttgatatcga agaaattttt tctgcgcaaa cagcgaccga
    27001 attgacagac aacattatta caccagcaga acacgagcga ctcgcagact gcggtttagc
    27061 cttttctctg gcgctgacac tggcattttc cgccaaagag agcgcattta aggcaagtga
    27121 gatccaaact gatgcaggtt ttctggacta tcagataatt agctggaata aacagcaggt
    27181 catcattcat cgtgagaatg agatgtttgc tgtgcactgg cagataaaag aaaaaatagt
    27241 cataactctg tgccaacacg attaattgac aacatctggt acgattcgcc cgcagccatc
    27301 actgaccgcg ggcgaaagtg taaagcaggt gccttaccac tctgacctga caaccggata
    27361 tgcggggatt gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta
    27421 aactcaatat tacgttcctt tacggtgaac tcacacagcg agtctccgac cagaagcgta
    27481 aatcccagta ccgtcaaaca cagcactatt actgccacaa gggcatattt cgtcagcatg
    27541 tttatattgc ctccatgcga agaagggact accatccgag ttgttgaggc tcggagtggc
    27601 agccccagga ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagttaac
    27661 ctgaaaccgg aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatatctg
    27721 tttatttata cagtgaatcc tgtttcggac ttctgtcggc aaaactcatc attgctctta
    27781 aaacgaaatc gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac
    27841 catcctgacc tgacaaccgg atatgcgggg attgctcccc gcacaacggc tacttcttcg
    27901 gttcgtaagc gagaacagcc ttaaactcaa tattacgttc ctttacggtg aactcacaca
    27961 gcgaatcccc gaccagaagc gtaaatccca gcaccgttaa acacagaact atgactgccg
    28021 caagggcata tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc
    28081 aaattgttca ggtttgggta ggtagcccca gggttgatag aaatatcgtc ctgggacttc
    28141 atctttccag acctcagagt aaacctgaaa ccggaaagct tcaggcaccc gcagttatct
    28201 tacccttgct tagcaaaata gctgtttatt tatacagtaa gtcctgtttc ggacttctca
    28261 ctccccattt gatcaactga agctgaaccc gtttttttct gcatgcctca atcgtcaaaa
    28321 ttcttcgcca ccagccagcc gtgccataac ttgtcacatt gcaaaagtag ccaaattgct
    28381 tcagtcacca tcaccaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc
    28441 cgacttctat actgaataga aaacgccaac ttaagagaaa cctatgccat tacccgattt
    28501 tcatgtttct gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc
    28561 gggctatgac ttaagccagg actcttcaat gctgattgac gcggttaaaa atgagatcac
    28621 ggccggagag gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg
    28681 ccgtgatatc aaccaggctg ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc
    28741 agccgcagac catcatctgg aaatttgcgg cggtggcacg cacccgtttc agaaatggca
    28801 gcgtcaggag gtatgcgaca acgaacgcta tcaacgaacg ctggaaaact ttggctatct
    28861 catccagcag gcgaccgttt ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga
    28921 cgccatttat ttgctgcacg gcctgtcacg gtttgtgccg cacttcatcg ccctttccgc
    28981 cgcgtcgccg tatatgcagg gaacggatac gcgttttgcc tcctcacgac cgaatatttt
    29041 ttccgccttt cccgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc
    29101 cctgtttcgc tgcctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga
    29161 tattcgcccc agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac
    29221 ccttagccac gcggtaaata tggcgggatt aattcaggcc accgcccact ggttactgac
    29281 ggaacgcccg tttaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca
    29341 ggcctgccgc tatgggctgg aaggcgtcat taccgatccg cacactggcg atcgtcgacc
    29401 gctaacggaa gataccttgc gattgctgga aaaaatcgcc ccttccgcac ataaaatggg
    29461 tgcatcgagc gcgattgagg ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca
    29521 gctgatgcgc gatttcgtcg ccgatggtgg ctcgctgatt gggctggtga aaaagcattg
    29581 tgagatctgg gccggtgact aagtagggaa ttgccttaca gaagaatatc cctgacaatg
    29641 gtgttttaac ttacatttaa caatgttatg aaacaccctt tagaaacctt gactaccgca
    29701 gcaggcattt tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg
    29761 ctgacgctgg cacaaaaact ggtgaccatg ttccatctga tggatcttag ccagctttac
    29821 actttattgt tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc
    29881 tttatctggc aacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc
    29941 ggcatacttt gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca
    30001 cgaaacggcg aaacgcctag cccttgagtt accctttgtc gagctttgct ggccttttgg
    30061 cccggagttc gatgttttta aaattggcgg caaaattttt atgctgtcgt cggagctgcg
    30121 cggcgtcccc tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcagat
    30181 atacccgagc attaagccag ggtatcacat gaataaaaag cactggattt cggtgtatcc
    30241 cggcgaggaa atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt
    30301 ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct
    30361 atagcgcatt tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa
    30421 attactttca catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac
    30481 taaggcattt gatgccagta aaaaacttac cccggaacag gccgagcaga tcaaaactct
    30541 cctgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac
    30601 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg
    30661 taaaatactt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
    30721 ctggctgaag ctggttgttg accaggaaga tgctgatggc cgctttgcca cgccggaagc
    30781 gaaagccgcg aacgataaag gtcgcaagtt cttcgctgat atgcaccgta aagatctgca
    30841 tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg
    30901 cgtggcggcg ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga
    30961 tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca
    31021 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacatcac
    31081 cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc
    31141 tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac
    31201 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc
    31261 acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt
    31321 ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg
    31381 ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt
    31441 atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg
    31501 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
    31561 gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
    31621 atatttcagg ggattaaact gatcggcgca atcctggtcg gcattttgat gatctcgctg
    31681 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg
    31741 atgttggttt ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac
    31801 gatatgctga aactgggcga ctggctggag atgccgaaat acggcgcgga tggcgcggtg
    31861 atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt
    31921 cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc
    31981 gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
    32041 gacgaaatgc aacgtctgaa taaagcgcac ttgttaaaac cttatttaac cagccgccat
    32101 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc
    32161 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg
    32221 cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta
    32281 ccgctcgaga tctacgcgtt tactaacacc gtggtgtggc tggaatatga aagcattcag
    32341 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
    32401 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
    32461 ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt
    32521 atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga
    32581 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
    32641 caatgcaaag atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct
    32701 gacgtcgcat cgctgcacga aaacgcagat gcgccagaca gatcataatc cagttcaaca
    32761 gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt
    32821 tgattaatac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac
    32881 cgcgacggct gacgcgagtc aaaaactttg gcgcattacc ctgcacagaa aggccaaaca
    32941 gcatgcggct gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga
    33001 cgaagttcag cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg
    33061 ggctactgtt ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgaac
    33121 cgatgtaaaa cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt
    33181 ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca
    33241 taattaccgc cagcgacaaa atcagcccat tccagccggt ggcaaagaaa ccaccgtagc
    33301 gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc
    33361 caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag aactcggttt
    33421 caccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag
    33481 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
    33541 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
    33601 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
    33661 ccaccatctc gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
    33721 ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag
    33781 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
    33841 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
    33901 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
    33961 gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg
    34021 cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat
    34081 catgccgcca atcatcggtg cggcaatccg gctcatcacc tctgaaccag cccccgttcc
    34141 ccacagaatc ggcagcagac cggcgataat caccgccacc gtcatcgctt tcgggcgcac
    34201 gcgcaggacc gcgccgtgat ataacgcctc atccagcttc tgctcgctga atgtttgtgg
    34261 attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc
    34321 aaattcggcg gcgaccccgg cgagggcgat aaagccagtg cccgtcgcca cggaaagatg
    34381 aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat
    34441 aatcagcaac gcttcgccca cccgacggaa cgccagatac aacagcacga agataatcat
    34501 caacgtcatc ggcaccatga gtttaagctt atggttggcg cgctccagta gctcgaactg
    34561 cccggagaat gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg
    34621 caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt
    34681 cgggcgcgca ttctcggttt tcagcattga cggtccggta gagactttaa tgtcggccac
    34741 gtctgccagg gtgatttgct gcttcatcgg cgtcaggatc ggcaactggc gcagtgcctg
    34801 cggactatcg cgccagcttt gcggataacg cagattaatt ggataacggg caatcccttc
    34861 caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact gcacatccgc
    34921 caccgtcata ccgtaacgtg cggctttttc acggttaatc tcaacgttga tatagcgccc
    34981 accttccaga cgctcggcta gagcagaagc cacgcctggc accgttcgcg ctacttcttc
    35041 aatttgctca gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat
    35101 ggggctttta atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag
    35161 attcgccagc cccggcagac gcacggtgtt atccagttcc tcaatgattt tgtccattgt
    35221 catgcctggc cgccactgct cctgcggctt aagctggatg gtcgtttcta ccatctccag
    35281 cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc
    35341 aggtacgctc ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga
    35401 aatccccggc agcgtcgatg gcatatacaa caggtcgcct tcattgatct gcggtaaaaa
    35461 ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg ctgccaccaa
    35521 cagcgtggtt ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa
    35581 aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc agtaacccat
    35641 caggataggg atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt
    35701 gaacgccagc gggccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa
    35761 cgacaacgtg ataatcagca gactgataaa tagcgccgga cccacttcaa cagatgcatc
    35821 ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca
    35881 ctcttccagc cgtttatgtg cattttcgat catgacgata gcggcatcga ccatcgcccc
    35941 gacggcaatc gcaatgccgc ccaacgacat aatattggca ttcagtccct ggaagtgcat
    36001 gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg
    36061 cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt
    36121 gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat
    36181 ctccacgcct tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat
    36241 cacttctcgg gcgtttttgc cggatcgcag gatcaccacc ccgcccgcca cttcgccttc
    36301 gccgtttagt tcggcaatgc cccggcgcat ctccgggcca atctggacct tcgcaacatc
    36361 gcgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc
    36421 gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga
    36481 cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat
    36541 gccatactgc gccaggcgct ggggatcgat aaccacctga tactctttca ccacaccgcc
    36601 caccgacgcc acttccgcaa cgtcagggat ggttttcagc tcatatttga gaaaccagtc
    36661 ctgcaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc
    36721 atagatccag ccaacacccg tggcatctgg tcccagctcg gcgctgactc ccgcaggcag
    36781 cttaccctgt acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt
    36841 gccatcttcg aaaatgacat acacataaga gtcgccaaac tgtgagaaac cgcgcacagt
    36901 cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac
    36961 gatttgtggt gcctgaccgg gatagctggt tttaataata acctgcacat cggagagatc
    37021 cggcagcgca tccactggcg tattaatgat ggtccaggtg ccccagatgc tcagaaacaa
    37081 cgcgcccatc agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat
    37141 tggttattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca
    37201 gaaatattgg cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc
    37261 gccagaccag agcgtaatgc ggtgacgcct tgcgatacct ggaaaacagc aacgcgtttc
    37321 ggtacaaagc gcccgtcggc atcaacggta atcacccgct gttcgctgcc ggtatcaatc
    37381 agcgcttgtg acggaatgag cagcatcggt tcgctggcgg tgttgagttg caaccaggcg
    37441 ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg
    37501 cgggtcgcgg catccacgcc aggtagcagc gtccatttgc ggatggtgag tgttttatcc
    37561 ggtcgcgccg gaacggtgag ggtaaactgc gaggcatctt tcaccagcca ggcgatggac
    37621 tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc gaccacgtta
    37681 tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc
    37741 gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc
    37801 tccggcattc ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg
    37861 ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcagg aatggtcagg
    37921 tcgagaagcg gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg
    37981 ataaacccgg cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc
    38041 gccgggaaac tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc
    38101 agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca
    38161 tatttcggca ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta
    38221 ttggggtaca tcgggtcgta ccagaataag attttacgtt ctgcggtcga cgttttttct
    38281 gcgggtggtt cagcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg
    38341 atcatgctgc cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg
    38401 actttaatat cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg
    38461 tcgccagttt taatttcact cattttcgtc tgcggagtga tggtaaagcg catggtcatc
    38521 tccggccagt tcacggcagc aatcggatcg tgatggatgg tgattttttt gctttccaga
    38581 tcaataccct ttaccacgcc agtggcgcta ataacctgcg gttgtacttc gctcatggtt
    38641 tcatgatgat gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt
    38701 gcgacttgca gtgctttttt catttattct ctcctggagt taaaagtcat actctaaata
    38761 attcgagttg caggaaggcg acaagcgagt gaatccccag gagcttacat aagtaagtga
    38821 ctggggtgag cgaacgcaga cgcagcacat gcaacttgaa gtatgacgag tatattgctg
    38881 tcaaccgcca cctaaggcgg tatacaaaga aatttcgtta acctgacggg cataattcag
    38941 gtcgagtaaa gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata
    39001 acttactgcg ccgtgctgat ataatgcccg cgcccgttgc aaagtaattt gcagcgacgc
    39061 cagataacgc tgctgggcgc tgatttgatc gttcaggctt tgacgtaatg ccagcgcatc
    39121 tgccacttct ttaaaggcgt tctggatttt ctgttcataa ttcactaccg actgctgctg
    39181 gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc
    39241 aattttagga ataaaattcc acatcccgct gctggcgtta aataatgacg acagatcgct
    39301 gctggcggtt gatattccgc tggtcagcct tatagatggg aaaaatgccg cgcgtgcggc
    39361 cccaatattg gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg
    39421 caataagatt tgcgacgaca atcccgccgg taatttaacg ctttgcaggc tgtcgctgtt
    39481 tactgtctgc gcttgcggca gcttgccgta gcttcccaat aacagttgca atgcattatt
    39541 cgcctgcgcc agttccccct gacgtttagc gatgtcgctg cgggtacttt caatcacccc
    39601 gcgagcctgt tccagcgcca gaacattact gctacctgtc aacagttgtt tttcgacaaa
    39661 cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc
    39721 tagctgctga ttgaaataac tttgcgcgac attagaaacc agcaaaatat gcaccgcgcg
    39781 ctgggcttcc tcagtggcta aataattttg gcgctcggct tcgctcatgt tctttaagcg
    39841 accgaaaaaa tcgagatcaa agctggcgtt aaggccggtc gaaaactccc gtgtcgtggc
    39901 ggtattccct ttaagattac cgctccagct gccgctgccc tcgccattga gctgtgggta
    39961 gcggtcggca tcggtcagac gatattgcgc ccgcgtttcc tgcactttca gcgtcgccat
    40021 gcgcaaatcc cggttattca ccagcgcctc gctaatcagc gtctttacct gattatcaac
    40081 aaaaaaggtg cgccagccca cgttctggta gttatctgct gcgttaacca agccgttctg
    40141 gctgagtgag aactgctgcg gcacgggcat tgccggacgc tgataatccg gtgccagtga
    40201 acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc
    40261 ataatttctg gtgattttat accgccaact ttactcgcca ggctctgatt ttccggtgac
    40321 aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct
    40381 agaattgccc gggcaacatg cggaggaaaa atgaaactgt tgattgtcga agatgaaaag
    40441 aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc
    40501 gacaacgggc tgaatggcta ccatctggcg atgaccggtg attatgatct gataatcctc
    40561 gatattatgc tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg ttccgccaat
    40621 aaagggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg
    40681 ctggagttgg gggcagatga ctacctggtg aaaccattcg cttttgctga actgctggcg
    40741 cgggtgcgca ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt
    40801 gccgatctga tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgcatcact
    40861 ctgaccagta aagagtttac tctgctggag tttttcctcc gccatcaggg cgaagtgctg
    40921 ccccgctcgc ttatcgcctc gcaggtgtgg gacatgaatt tcgacagcga cactaacgcc
    40981 attgatgtag cggtgaagcg gctgcgcggc aaaatcgaca acgactttga gccaaagcta
    41041 attcagaccg tgcgcggcgt gggttacatg cttgaggtgc cggatggtca gtaagccatt
    41101 tcagcgcccg ttttcgctgg caacccgcct gacctttttt atcagcctgg ccaccatcgc
    41161 agcgtttttc gcctttgcat ggatcatgat ccactcagta aaagtgcatt ttgccgagca
    41221 ggatattaat gatttaaaag aaattagcgc cacgcttgaa cgggtactta atcatcctga
    41281 cgagacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt
    41341 gttgatctcc ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgcctga
    41401 tatccgcgag tttgcacgtg atgccatacc cgataaagac gcgcggggtg gcgaggtgta
    41461 tctcctttcc ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag
    41521 caactggcgg atgattaact tgtcggttgg cccgttggtg gacggcaaac cgatttatac
    41581 gctctacatc gcgctttcga tcgattttca tcttcattac ataaatgatt tgatgaataa
    41641 acttattatg accgcatcgg taatcagcat cctgatcgtc tttatcgtac tgttggcggt
    41701 acataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa
    41761 agatctcgac gttcgccttg acccgcagac cgtgcccatt gagctggaac agctggtact
    41821 gtcgttcaac catatgatcg agcgtattga ggatgtcttt acccgccagt ccaatttctc
    41881 agcggatatc gctcacgaaa ttcgcacgcc gattaccaat ctcatcacgc aaacggaaat
    41941 cgccctcagc cagtctcgca gccagaagga gctggaagat gtgctctact ctaatctcga
    42001 agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa
    42061 caaccagcta atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt
    42121 cgattttttc gaggcgttag cggaagatcg cggcgtggag ttgcgatttg ttggcgacga
    42181 atgtcaggtt gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa
    42241 tgccctgcgt tatacgccgc ccggagaggc aattgtagtg cgctgccaga cggtcgatca
    42301 ccaggtgcaa gttaccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg
    42361 attgtttgac cgtttctacc gcgttgaccc ttcccgccag cgaaaaggcg aaggtagcgg
    42421 cattgggctg gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcagtaac
    42481 gtcagatgcg cgggggacaa ggtttgtgat tatgctgccg gagagagagt gattattaag
    42541 ttcaaattca gggcgtggtg catcacctcc tggtattgtt gatcaacaaa ttagagaaaa
    42601 acttattaaa catatgaaat atcgaaggct tttatttttc aaagtttcac gagatgtttg
    42661 tatatgaaac ttagttaaat ctgtgtttct tccaattgta gatcacaatg ccctttctaa
    42721 a
//
LOCUS       DAZKGS010000020        41064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKGS010000020 DAZKGS010000000
VERSION     DAZKGS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(109..4011)
                     /gene="hrpA"
                     /locus_tag="ACHOQY_001142"
     CDS             complement(109..4011)
                     /gene="hrpA"
                     /locus_tag="ACHOQY_001142"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9677283.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILLMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4212..4817
                     /gene="azoR"
                     /locus_tag="ACHOQY_001143"
     CDS             4212..4817
                     /gene="azoR"
                     /locus_tag="ACHOQY_001143"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9677284.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4868..6184)
                     /locus_tag="ACHOQY_001144"
     CDS             complement(4868..6184)
                     /locus_tag="ACHOQY_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP9677285.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6174..7931)
                     /locus_tag="ACHOQY_001145"
     CDS             complement(6174..7931)
                     /locus_tag="ACHOQY_001145"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP9677286.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(7947..8843)
                     /locus_tag="ACHOQY_001146"
     CDS             complement(7947..8843)
                     /locus_tag="ACHOQY_001146"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9677287.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8843..9448)
                     /locus_tag="ACHOQY_001147"
     CDS             complement(8843..9448)
                     /locus_tag="ACHOQY_001147"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9677288.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(9619..11925)
                     /locus_tag="ACHOQY_001148"
     CDS             complement(9619..11925)
                     /locus_tag="ACHOQY_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP9677289.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(11989..12849)
                     /gene="pdxI"
                     /locus_tag="ACHOQY_001149"
     CDS             complement(11989..12849)
                     /gene="pdxI"
                     /locus_tag="ACHOQY_001149"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9677290.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(13080..13670)
                     /gene="paaY"
                     /locus_tag="ACHOQY_001150"
     CDS             complement(13080..13670)
                     /gene="paaY"
                     /locus_tag="ACHOQY_001150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP9677291.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(13652..14602)
                     /gene="paaX"
                     /locus_tag="ACHOQY_001151"
     CDS             complement(13652..14602)
                     /gene="paaX"
                     /locus_tag="ACHOQY_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFP9677292.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(14703..16016)
                     /gene="paaK"
                     /locus_tag="ACHOQY_001152"
     CDS             complement(14703..16016)
                     /gene="paaK"
                     /locus_tag="ACHOQY_001152"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFP9677293.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(16043..17248)
                     /gene="paaJ"
                     /locus_tag="ACHOQY_001153"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(16043..17248)
                     /gene="paaJ"
                     /locus_tag="ACHOQY_001153"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFP9677294.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(17248..17670)
                     /gene="paaI"
                     /locus_tag="ACHOQY_001154"
     CDS             complement(17248..17670)
                     /gene="paaI"
                     /locus_tag="ACHOQY_001154"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFP9677295.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(17660..19087)
                     /gene="paaH"
                     /locus_tag="ACHOQY_001155"
     CDS             complement(17660..19087)
                     /gene="paaH"
                     /locus_tag="ACHOQY_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFP9677296.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(19089..19877)
                     /gene="paaG"
                     /locus_tag="ACHOQY_001156"
     CDS             complement(19089..19877)
                     /gene="paaG"
                     /locus_tag="ACHOQY_001156"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFP9677297.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(19877..20644)
                     /gene="paaF"
                     /locus_tag="ACHOQY_001157"
     CDS             complement(19877..20644)
                     /gene="paaF"
                     /locus_tag="ACHOQY_001157"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFP9677298.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(20641..21711)
                     /gene="paaE"
                     /locus_tag="ACHOQY_001158"
     CDS             complement(20641..21711)
                     /gene="paaE"
                     /locus_tag="ACHOQY_001158"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFP9677299.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(21719..22216)
                     /gene="paaD"
                     /locus_tag="ACHOQY_001159"
     CDS             complement(21719..22216)
                     /gene="paaD"
                     /locus_tag="ACHOQY_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFP9677300.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(22231..22977)
                     /gene="paaC"
                     /locus_tag="ACHOQY_001160"
     CDS             complement(22231..22977)
                     /gene="paaC"
                     /locus_tag="ACHOQY_001160"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFP9677301.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(22986..23273)
                     /gene="paaB"
                     /locus_tag="ACHOQY_001161"
     CDS             complement(22986..23273)
                     /gene="paaB"
                     /locus_tag="ACHOQY_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFP9677302.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(23285..24214)
                     /gene="paaA"
                     /locus_tag="ACHOQY_001162"
     CDS             complement(23285..24214)
                     /gene="paaA"
                     /locus_tag="ACHOQY_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFP9677303.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            24499..26544
                     /gene="paaZ"
                     /locus_tag="ACHOQY_001163"
     CDS             24499..26544
                     /gene="paaZ"
                     /locus_tag="ACHOQY_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFP9677304.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            26792..29065
                     /gene="tynA"
                     /locus_tag="ACHOQY_001164"
     CDS             26792..29065
                     /gene="tynA"
                     /locus_tag="ACHOQY_001164"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFP9677305.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(29123..30622)
                     /gene="feaB"
                     /locus_tag="ACHOQY_001165"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(29123..30622)
                     /gene="feaB"
                     /locus_tag="ACHOQY_001165"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFP9677306.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            30858..31763
                     /gene="feaR"
                     /locus_tag="ACHOQY_001166"
     CDS             30858..31763
                     /gene="feaR"
                     /locus_tag="ACHOQY_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFP9677307.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(31935..32264)
                     /locus_tag="ACHOQY_001167"
     CDS             complement(31935..32264)
                     /locus_tag="ACHOQY_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9677308.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(32269..32454)
                     /locus_tag="ACHOQY_001168"
     CDS             complement(32269..32454)
                     /locus_tag="ACHOQY_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9677309.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(32451..35090)
                     /locus_tag="ACHOQY_001169"
     CDS             complement(32451..35090)
                     /locus_tag="ACHOQY_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9677310.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            35298..36287
                     /gene="ldhA"
                     /locus_tag="ACHOQY_001170"
     CDS             35298..36287
                     /gene="ldhA"
                     /locus_tag="ACHOQY_001170"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9677311.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            36398..36820
                     /gene="hslJ"
                     /locus_tag="ACHOQY_001171"
     CDS             36398..36820
                     /gene="hslJ"
                     /locus_tag="ACHOQY_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9677312.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(36817..37083)
                     /locus_tag="ACHOQY_001172"
     CDS             complement(36817..37083)
                     /locus_tag="ACHOQY_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9677313.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            37357..40881
                     /gene="nifJ"
                     /locus_tag="ACHOQY_001173"
     CDS             37357..40881
                     /gene="nifJ"
                     /locus_tag="ACHOQY_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9677314.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        10270 a        10987 c         9864 g         9943 t
ORIGIN      
        1 ttgtattttt ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc
       61 ggcgctaaat gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat
      121 ctgctccatc gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg
      181 agcgaagtaa ctaacgcgca actcttctat catccaacgg atctctttca cgtcttcatc
      241 ctcacgacgt gcgggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc
      301 gactttcagc atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt
      361 ttcaatcgcc tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac
      421 aaaaccgcga tataccaacc cgcccatctg cgctttaatg tcagaaagcc ccagtgccat
      481 ggtcatatcc acccgccctt tcagacgttt gttgatattg aacaccgcag tgaggatttg
      541 ctcgacctgc ttcgcaatat ccaccaccgt gtcattcagt tcggcgcgca ctttttcatg
      601 cagcgcagca aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaattgatc
      661 cacaccgcag gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta
      721 cagtcccagc ttggctttgt tcggtaactt ttcatgtaaa tatttgattg gcgatggaat
      781 attcagcagc agtagacggc gaagaccgtt ccacattgcc tgcttttgct ccagcgggtt
      841 atcaaacagt ttgatcgcca cactgtcgcg ctcatccacc agcgccggcc acgccttcac
      901 tttgtagttg ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg
      961 taagccgctc tgctcgatac cgtcatccgc caccgcagat agcgtttcct gcactttgcc
     1021 tttcagcgca tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc
     1081 atccaccacg cggaaggtaa ttttcaggtg atcaggcacc tgatcccagt gccagtcttc
     1141 gcggtcaacg gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg
     1201 taactccagc ggtttgacgc ggcctaaaaa cgcttcggca tagtttggcg cgggtacaaa
     1261 attacggcgt accggtttcg gcaacgattt aatcagagca atcaccagtt cgcggcgcag
     1321 accggggatc tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat
     1381 atgtacggtc acaccgtcag catccgcgcc gggctcaaac tgatagctca aacgcagctt
     1441 gagattgccc tgatgccaga agttcgggta atccagcttg ctgatttttt ctgcgccctc
     1501 tttgatcaac atgctttttt caaagttgag caaatcaggg gtttcgcggc tgactttttt
     1561 ccaccagctg tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa
     1621 ctcaaacaac gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc
     1681 ttccacttcg gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc
     1741 accttccacc agcgcatggc gaataaagag ttcacggcat aacgccggat cgatctggct
     1801 gtagttgacc ttgcgcgcgg caacaatcgg caaaccataa acagtgactt tttccgttgc
     1861 catcaccgcg ccctgcgccc gttcccagtg aggttcgctg taggtgcgtt taatcaaatg
     1921 ctgagcgaca ggctccaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg
     1981 gctggtttct accagttccg ccaccattac ccatttcggc ggttttttga ataaaccaga
     2041 accgggaaag atggagaaac gcgcgttacg tgcgccggta tattcttgtt tatcggcatc
     2101 tttcatgccg atatgggaaa gtaaacccgt cagcaacgca atgtgaattt cgcgatactc
     2161 cgccggttcg ctgttaaccg gaatgccaag ttctttcacc acctggcgca actgggtgta
     2221 gatatcctgc cattcgcgca cgcgcagata gttgagataa tcgatacgac acaggcgacg
     2281 gaaggcgttg gaagaaagcg ccttttgctg ctcgccaaga taattccaca gattcacaaa
     2341 cgcgaggaag tcagactctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg
     2401 tttatccatc ggacgctcgc gcggatcctg aatggaaagc gcggacgtga taatcatcgc
     2461 ctcacgcacg cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtggatcgac
     2521 aggcaactgc gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc
     2581 cgtggtgatc gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc
     2641 cggtgcttcg acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatcagcaa
     2701 aataaccgag gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgaaag
     2761 gaaatcgtct tcggaataga gacgaataca gatcccttcg gacacacgcc cacagcggcc
     2821 tttacgctga ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt
     2881 gcgatagctg tagcggctga tacgtgctgt accggggtcg ataacgtatt taatccccgg
     2941 tacggtcagc gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga
     3001 ctggaacacg cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt
     3061 atgacgcaga tttagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact
     3121 cataaagatc agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat
     3181 cgcctgcaac tgatcgcgct cggtgtcgtc ggcttcttca acaatcggtc gatagcgcac
     3241 ttccaccgga taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga
     3301 aaagcgttcc gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg
     3361 cagcaactct ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgtgcttc
     3421 gtcaataatg atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag
     3481 gataccgtcg gtcatcagct tgaccatcgt gttatcacta acgtgatcgc tgaaacgcac
     3541 tttgtaaccg atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac
     3601 cgttcttgcc gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc
     3661 cagctccata cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac
     3721 gatcaccacc tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg
     3781 taaattgtca ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc
     3841 cgcctggtca atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt
     3901 aaccttctta acaccgtgca gacgccgaga aaaacgcagt ctgtcgcgca gcatcagcga
     3961 atccagccgc tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt
     4021 gggcagtagt ctgcctgatt aattttcttt taaatgatag atatagagta ccacatcgcg
     4081 gctttctatg cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg
     4141 tggcaggatt gtgaataaag tgtcagcaag caacggggca tcgcccattc aaacatctat
     4201 aaggaaacac catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc
     4261 agtctaatca gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg
     4321 aaatcaccgt tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg
     4381 gtgcactgcg tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt
     4441 ccgatgagct gattgccgag ctgaaagccc acgacgttat cgttattgcg gtaccgatgt
     4501 ataacttcaa catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg
     4561 ttactttccg ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg
     4621 ttattaccag ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc
     4681 tgtccacgtt cctcggcttt atcggcatta ccgatgtgaa atttgtcttc gccgaaggga
     4741 tcgcatacgg tccggaaatg gcagcgaaag cgcagtctga cgcgaaagcg gccatcgaca
     4801 gcattgttgc tgcataaccg gacacgatct tgatgtggac tgtagttcag tccacatctc
     4861 aatccactta cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa
     4921 tatgcgaacg tctggctcga atataactaa tcgcttcatc aacggtttta cagtgtccgt
     4981 aacataacaa ccatgccgcc actaccagcg cactgcgcga taatcccaac gcgcaatgga
     5041 ccagaacgct gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct
     5101 ccccctcttc cggaaccacc agatccagca tcggtacaca gaaatagagt cgatcttttg
     5161 tcgctcgtcc ccgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat
     5221 gtcgtggaaa cgcccctaaa taaacaccag cagtaatttt gctcaccggc tccaggcgac
     5281 gacaaaacca acgcatagac agccacatcc cgatgcgcca gggcaatgtc agccagtaaa
     5341 cggcgggcgg gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt
     5401 acccacggcc aataatgagt agcgataata ctggccaaca taaccagact gaccaccata
     5461 actgaatcat caccattagc tccatcaaca caatgcacga gcccgcgcct acgacatatg
     5521 gcagtgctat tttgattcga cgttgatcag gtttctgata attccaacga cgatcgacgg
     5581 gcaccatcca gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat
     5641 gctgccaggt cgtcagagta gaaatagcga tgagtaaaaa ccatccgcca cagactttac
     5701 gccacctctc agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat
     5761 gcagcgaagg agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc
     5821 ccgtcacccc actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac
     5881 cgcagcaggc cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg
     5941 tcgtgctaca aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa
     6001 tagtccaggg aagaaaaggg atcgccgttt cccaaccgaa tacctgactg gggatatcat
     6061 ggctgttaag gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa
     6121 acggggccag caacaataac cagccagcgc cttgtagcaa tacgttccga cgttcagtta
     6181 tcacgacgca ccgccatcga aaccgtaaaa ataccccact catcaatccg ttgtgtgcat
     6241 ttatcaaatc cggcatcacg cacgagtgaa tccatctccc cttgcgaacg cacgcgcatt
     6301 acccacggtt taccatcttt atgactggtt aacaccccgg caatcatctc cagttgaggg
     6361 tgccacggct gcccggtgta gatgagaatg ccgcccggtt cgatggcatt ggcaagacct
     6421 gcgagtgagt tttttacctg ctcattttcg ggaaaaagct catacaggcc agagacaatc
     6481 gccagcgtag gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt
     6541 tcaaaacgca cccgcccaga cattccccgt tgggcaatca tcgcttgccc ctgtgcaaca
     6601 tttaactcac tgtaatcacg taacaaaata tcgcttacgg caggctcgtt tgccagcgca
     6661 tccagtacat agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg
     6721 gcgtggagat cggcaacggc ttgtttaatc agtatttgta aatgggtttt acgctggcga
     6781 ataccgcgcc agccaacact gttcaggtag attttgtcaa ttaagcgccc gaatgcgtta
     6841 ctgccttgcg gttgattttg atagacataa tccagggaac tgcctgaatc aaagccggtg
     6901 ctcattccga gatgcaggcc cgcagagtgc gccccgaaca atttcatcgc ctttcgcata
     6961 aagcgatacg ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gagccgccag
     7021 cgatccgctg atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta
     7081 gcatataaac gactaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca
     7141 cccaacgtgt cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat
     7201 ctctgataaa aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga
     7261 agttgcgtgg ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttgtag
     7321 agatcgagca agatattaac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc
     7381 gccacccgtt gccgatcgtg ggtcaaatag cgtcctttca cataggaatt aataaaaaac
     7441 agaccacgca gacgatgcca taacgccagc gcaggacgtg ccagcggtac atacaattta
     7501 accttaaagg ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc
     7561 catgtggcaa ccagcactgc gccgacgctt tgcgcgatca ctaccacctc atccagtccg
     7621 acctggctat cgctggcagc aaaacgaaca aattcatcga catcctgcac tgaacgcgca
     7681 agagatgggc tataaccacg cggccccgaa gtttgtccat gccctcgggc atcccaggca
     7741 taaaaaacag tatctggcat cgccagttca tcaacgagat gttgtagacg accagaatgt
     7801 tcatgaccgc gatgaaataa gacgatgacc tttttagccc cgggctgtaa agcgggccag
     7861 tgccgataaa acaacgctgt attatcactg ctggtaaaaa aatgttcccc atggatgcgt
     7921 gaattttcca ttttctctat tttccttcag taacagcagt agcgaataaa ataaaaaaat
     7981 accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg tcctggtaat
     8041 agtttcccac tatctttaac accaatatct cgtttgatgg ctgacatcac gacatcgccg
     8101 caaaaaccac taataccaat taacaaaccc gctaataatg cctgtaatgt atttagcggt
     8161 gtcagcaacg gtccaataat cagtgacgca atcatggtgg tgattacgcc ccccaccaga
     8221 ccttccagcg tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc
     8281 ccccataaat actgtgcaat atcgtttgac tcggttaatg ccaaaagaaa taacaccagt
     8341 aacgccccgc cctggatatt tgtggttggc aacattaata accaggcggc atgactcaag
     8401 gcgaaaacgg tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag
     8461 gggtccccca taaaaacttg cccggtggct aatattagaa acccagccaa aggtataaat
     8521 aatagataca gctcaaagca attaaaaccg ataaacaaat agttaagagg aataacccaa
     8581 tataaccacc gcggaaaatg tacagatata agcgtacaat attctttcaa tgccagaaaa
     8641 cttatcagcg caaaaaacgt caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa
     8701 caaatgacga tgccccacca ggtacgaata cgtaatgtaa cttcatcctc ttttctctcc
     8761 ggtaaccgcc atagcagaaa atggtttatc agcgttgcta aaattaacaa cgcgaaaagt
     8821 gttgccagag atttttccag cattatcttt cagcgctcat aagaagcaca ctccgacaac
     8881 gattaatcgc agtccagaga agcagaattg aagcaatgct ccataaaaga ttattccact
     8941 gcatccactg tggataaatg gcaacggcca gaccccacag accaaatatt aacgcgcgat
     9001 cgcttttgcc aaatggaccg gcataactgc gaacaccgtt aatcgtctgc gcgagtaaac
     9061 cgcaaaactc ggtcaatatg gtgcaaaata acatgagtat cacgagtgat gcgttacttt
     9121 ccggtaaaaa taaaaatggt aaatagagcg caatatcgga aataacatca ccagtctcat
     9181 tcaaaattgc cccaagccgt gtttgctgat tgcactcacg cgccaacatg ccatccagcg
     9241 cattgagcgc catacggata aaaagcacga tgggcaatag cataaagagg atgggttgtg
     9301 ccaccaacat caacagcaat ccggtaagaa gagaaagcgc cagtgcagca agagtgatgt
     9361 gattcgctgt aacgtggtgc ttgtaaagcc aaaacatcgt cggccttaac agcgactgaa
     9421 agagcggttt tatctggtag agcgtcatta tcaatccttg ataattcggt ttgcgctatg
     9481 gtgcatcaat tcccctgacg gagatatcgc aacacgccgc ttcattaaaa attcagaata
     9541 tatcaaggta ttggggactc actgtcagga ataattatcc ccaattaatc aaagggttaa
     9601 gaaatattac gttgtggatc aatacaactc atcaagtttt ctcatcgctt gttcccgtat
     9661 tgcgcgcgtc ttaccgttaa gcgccagctg gcggactaaa tccagttcag ggttaacaag
     9721 ctctaacgcc agcaaaggat cacgtttcag cctcgccttt aatacatcaa ctggaaaacg
     9781 gggattattc atcgccacaa tccaccactc gggatcaatt tctgccgcca gtactgcagg
     9841 cggtgtgtgg cggttgttaa gtgcgccgaa cttgaaaaat gtgagattgc ttttcgccag
     9901 tgtactccac catatacgat ccggttgttt tgccagtaca tccagttgtg ctaacgcatc
     9961 ggggtcgagt gcataataga cgcgtaatgc ttcatcaacc tgcgcacggg agctgttttc
    10021 ctgcctcaga aaaaacttcg cccaaatatc attgcgtggc gtattttgct gtaattcctt
    10081 gaggacaaca gcgggtaaat tatcccaact aactaatgca ctctctaaaa agtcccagtc
    10141 ttccaaacgt atcagggcgg tggcatttaa tagcatctga tcatcaactt caccgtggct
    10201 gataagctca gacaaaatca tatcagcgct ggtttcatac gcagctgaat ctgctttcgg
    10261 attatttttc cgatgcgata aagcacgttg ccaaattttt tgtttcatcg acgtgctcag
    10321 atactgattt ttcgccagat tactccaccc ctcaggatcg ttgaaacgtg tcaataaccg
    10381 ttgtagagca gcgtcagagt gaattacctg catatcccga cagcaaaagt aggcagggga
    10441 ttttttctcc atccttttaa caaaattaaa ctgtaagtta tctggcaatg ctaaaaataa
    10501 cacacgtatt agatcatcgt cattctcgaa tttttggtat aaataggctg caactttgtc
    10561 actctcttgt ggcgtcatcc gcaataattg accatggtgt ttcatctctg ccagcttgaa
    10621 cgctaagctt gtgacaacat tgtttgatga atcgttcgcc aactgcattg cctgattgta
    10681 ggtgagttgt cctccagcac cgacctgacg aatagaggaa tcatcactgc ggatatagcc
    10741 atcaataatg gattcaggca ataaccacgc atctttataa agctcctgaa tgatcatatc
    10801 ccaatctctt ttcgccagag tcattacgat atccagaggg agattttcac gtagcgcaag
    10861 attcaacgga ccgacaccat caggtagacg actcattcgc gtcattattt cgacagagag
    10921 cggttcacgg tcgagcaacc ccagaacaac aggctggtaa cccaattgct gatcggtcaa
    10981 ctccagaatc tcattgtcag gcatatatct cgccatactt tcaatcgttt gttcatcatt
    11041 gctattaata agttgtttgc gtaggttagc tgaaattcct ttaagccttg ggagagttcg
    11101 cattatggca agatcaaatg tcgcaatatc tcgtccttca aatgcagcaa cgttgccttc
    11161 atccagcatt tgcaattcct gcactaactc aggagaaggc ttagggcgaa acagcgcttc
    11221 ttttttcatc tgattacaga cattccctgc catgcgaata ttgcttcgct taaaaatttc
    11281 tttcagagca tcttcatcat ttattagacc ccaataatat gcatcaggat ttattatcat
    11341 cagcgctttt ttgacatttt cagtgtcatt tttgtcactc gcacggacca tgaattcact
    11401 atttgtcgaa gacgcaacaa caggtgcaat tatgtcattg ataaatattc cacgcgcatt
    11461 aatactcccc ttaatcgaag acggattatc gtaatatgag tcgataaata ccagtccact
    11521 tgcatttatc gaaccggtaa ttaacagtgg tgaatccgta actatattcg ttgccgtcac
    11581 gttaccgaga ataacccatg gattagaaag agagaaagtg gaaatattac ctttgactgt
    11641 caaattacca agaacaatca atttttgatt actctctata ttaccttcta ccactaaatc
    11701 gccacgatgc actttccagt ccgatgcaga taaaccgaag gggacaagat tcacgctact
    11761 gttgatagct tgttcaactt gttgtttatc ttcagcggta gccgcagaaa aaaatggttc
    11821 aatatcaact gtatttgcaa cacatcgaag ttccgcctgt actggaagac tcagcataaa
    11881 caggggaagt aaacctatcc agccgcaaat cctggactta aacatatgca ctccatgcca
    11941 ttaaaaaaga aacaaaaggg aaatgcatct ttcccttttg tatattcgtt attctcgcga
    12001 aataccatcc aacgtagaca acacttcctc agaaagatgc aatttttcag cagccatatt
    12061 ctcccgtaaa tgcgcaacag aagacgtccc tgggatcagc aaaatattcg gtgaacgttg
    12121 taacagccac gccagcgcca cctgcatggg tgttgcaccc aggctcgcag caacatccga
    12181 cagcgtagag gattgcagcg gtgtaaagcc cccgagcggg aagaacggca cgtaggcaat
    12241 gccatcgtgg gccaaagcat caatcattgc atcatcagca cggtgcgcga tgttgtattc
    12301 gttttgcaca cagacaattt cggcaatctt gcgcgcctct gcaacctgcg tcggtgtgac
    12361 gttgctcagg ccaatatgtt ttaccaggcc ttgttgttgc atctctgcca gcacggtcag
    12421 gctggcctca atcgatcctt ccgctgggcc atgaccatct cccatcataa cgcgcaggtt
    12481 aaccacatcc agtacgtcca ggccgagatt acgtagatta tcgtgcaccg ctttttgcaa
    12541 ctctgccgga gaaaaggcgg gcaaccagga agcgtcctct ccacgccgcg caccaatttt
    12601 agtgacaatt gtcaggtcgt cagagtaagg ataaagcgct tcgcggataa tctgattggt
    12661 gacgtgcgga ccataaaagt cgctggtatc aatgtgattg acgcccaatg ccagcgcctc
    12721 gcgcagcaca gttatagcga cgtggcgatc tcttggggga ccaaaaaccc caggacctgc
    12781 cagttgcatc gcgccataac caagacggtt aacggatttt gtaccgagag taaatgtatt
    12841 gctgctcatc gcaccctgcc ctgtaataaa acggattaag aaaaatcagt atagaaacga
    12901 aaagagataa ataatgcgag ggcgtaaaca agattactgg atgagttgct ctattgtcct
    12961 gaaaaacaca aaacaaaacg cgtaaagcgc cgcatccggc aataaaaaaa ccgcaacctg
    13021 tatcagcgtt gcggtttagg tatcacatgc taaaaattag aatgttcagt atctggacgt
    13081 tactgtttcg gtcgcagatt ctcatcaaat accagacgtt tcctgttcgg ttcaacttcc
    13141 cgcaatggct caacttgatg caacgtctgc ttacaacgag tcaccagcac ctgatactca
    13201 tgcgtaccct gctttttcca tgccagctcc tgctcactga gttcacgaat cgctttcgcc
    13261 gggctgccga caatcaggta attggcaggc atttctgctt tggctttcac aaatgcggat
    13321 gcgccaacaa tgctgttctc gccaatcacc gcgccgtcca tcactaccgc gttcattccc
    13381 actaatgcat tgcggcggat aatgcaacca tgaaggatag cgctatgacc aatatgtcca
    13441 tcttcttcca cgacagtatc ctgctcggga aaaccgtgca taacgcaatt atcctgaatg
    13501 ttcgcgccat ctttcaccac gatacgacca aaatcgccac gcaggctggc atttggccca
    13561 acgtaaacgc ccttgcctag aataacatcg ccgatcaata ccgctgtcgg atggacaaaa
    13621 ctctcttctg gcacaaccgg agtcagacca tctatctggt aaattggcat atcgcctcct
    13681 gttcaatatt caaaccgcca aaacgttgaa aatacaggct tcccggcgca ggcagttcac
    13741 cgaccgaggt ttcacctttt tcactaacga acgctaaagc agcaggcgct acgcgctgat
    13801 aaatgttgat acacagctga cgcgccgtat gccctgccca gtgcgccgga agcaactcct
    13861 ctggcaacaa tgggtcttta aggacgacac ggcgataaaa atggatcagt aaaagctgaa
    13921 tatgaaatgc acgctccggg gttaactcgt ctgctgcctc ttttaacagc ggcaccagcg
    13981 ggcggaatga ctgaataaag gtttcgtaca tggcattttg ttcagttaaa tgccagcact
    14041 cttccactct ggcacgcagt gctgcacgag aaagcgccag tggtatttgc gcttcaaaac
    14101 aaatcacgtt atccgccacc cccgcttcat gcaaaagtgt ctgtacatcc gccagttttt
    14161 gcgacggcga tgccatcagg ctgggtgcca gtgcgccaaa accttgccag atcaactgct
    14221 ttttgacatc agccagcgtt gatttatcta aaccttccga gagcaacagg agccatttac
    14281 catcccatgc aggttgctct gcgcgataaa ttttactttc tgcccgtcgc gtcaggcgca
    14341 agcctttatc actgaggcta tagaaactgc gtcgcccgat gcgggaaaca tccagccagc
    14401 cttctttatt aagacgaaac aaagcggtgc gcacgaaacg ctcaccaaat cccagccctt
    14461 ccagcaaagc agccagacta cccagccaga tttcaccacc acgatgggaa agcgaatcac
    14521 catacagaga ggagatcaaa gatgtgccac tgaccggaac agcgtttaca gcatgttgga
    14581 taaaagtaac aagtttactc attcgattca gtctgttgtt attgttgtga tttatgaatc
    14641 atagcacagg tcgtatcgac ttctggccct ggcgctttac ctcaccaggg ccagacgaca
    14701 cgtcaggcac caacaatatt gcgcagatca aacacccgac acgccttgcc ttctgaacgc
    14761 gggatactgc cacagttaac gatcatcaca tcggtagaga tccccaccat cgacttaatc
    14821 cgatggcgca gctggtggca cacctggcaa cgctgctcat gcgtcaatgt caggctgctt
    14881 tctttcaact ccactttcac agaaagtgaa tcaagatgcc cccggcggtt cacctccagt
    14941 tggtaatggg gcgataaatg ttcgaatttg acaatctctt cttccagttg tgacgggaag
    15001 acatttacac cgcgaatgat caacatgtca tcgctgcgtc cgctgatgcg atccatgcgg
    15061 cgcatagtcc gcgccgttcc tggtaacagt cgggttaaat cacgcgtgcg gtaacgaatg
    15121 accggcaatg cttctttggt cagcgtggtg aataacagtt cgccatgttc accatcggca
    15181 agcggtgtgc cgtcatgagg attaacaatt tcaggataga aatgatcttc ccaaatggtt
    15241 gggccgtcgg tagtttccag acactccatc gccacccccg gccccatcac ttctgacagg
    15301 ccataaatat ccagtgcggt gatccccaga cgccgttcaa tctctttacg catggcctgt
    15361 gtccacggct cagcaccaaa cactccaacc cgcagcgagc aaccgctggc atcaccgccc
    15421 aactgccgct ccagctcttc aatcaggtta aggcaataag atggcgtaac catgatcata
    15481 tctggttgaa aatcgcggat cagttgcgct tgtttttccg tctgcccacc agacatcggg
    15541 atcaccgtag cgcccagacg ttcggcacca tagtgcgcgc ccagcccacc agtaaacagg
    15601 ccgtaaccat aggcaacatg aattttgtct ttgggcgtgc cacctgccgc acgcaaggaa
    15661 cgcgctacaa tattggccca gttatcaata tcgttttgcg tatagccgac aactgtcggt
    15721 ttacctgtgg ttcccgaaga agcatgaatg cgcaccactt gttccatcgg cacggcaaag
    15781 gtgtcgaagg gatagttgtc gcgcagatcc tgtttggtgg tacagggaaa tttacgcaga
    15841 tctgaaagtt ccctgaaatc atcaggatgt acgcctgctg cgtcaaattt gcgccgatac
    15901 atcgggacat tttcatacgc gtgtttgagc gtccatttca gacgttgtgt ttgcaacgcc
    15961 tgtaactcat caacagacgc ggtttcaatc gggtcaagct ttgtattggt tatcattgta
    16021 gggtactcgc aggttgatat gctcaaacac gctccagaat catggcgatg ccctgaccga
    16081 caccgatgca catggtgcac aatgcgtaac gaccgttacg ccgatgcagc tcatggctgg
    16141 cagccagtgc caggcgggca ccactcattc ccaacggatg gcctaaggcg atagcgcctc
    16201 cgttggggtt aacatgtggg gcatcatcag gcagccccaa ttcgcgtagt acacccaacg
    16261 cctgggccgc gaacgcttcg ttcagttcaa tcacgtccat atcgtgaata ctcagccccg
    16321 cgcgttccag cacccggcga gttgcaggca ccggaccaag ccccatcagg cgcggttcca
    16381 ccccggcggt tgccatggct acgattcgcg cccgcggtgt cagtccttgc gctgctgcca
    16441 tctgttcact ggcaataatc aacgcagcgg ctccgtcatt caccccggag gcattgcctg
    16501 cggtaatcac cccattggca cgaaatggtg cttttaaccc acgtaactgt tccagcgtcg
    16561 tttccgggcg cagatgctca tcatgttgta tttctgttac aacgcctttc ttgtttttca
    16621 acacaaccgg aacaatctcc tcagccagaa tgcctgagga ttgcgctttt gccgtacgtt
    16681 gctgactgcg tagcgcaaaa ctatcttgat cttctcgcga tatttttaac agttcagcta
    16741 cattctctgc cgtttccggc atgctgtcag ttccaaattg ttgagccatg agcgggttca
    16801 caaatcgcca gccaatagtg gtatcaaaca tctcagcctg acgagaaaat gcactggttg
    16861 ccttgcccat aacaaacggt gcccgagaca ttgactccac gccaccagcg atcagcaaat
    16921 cgccatcgcc cgctttaatc gcccgtgcgg cgaaccccag tgcgtccagc ccggaaccac
    16981 acaagcggtt aatggttgtg ccggaaacac tatgcggcag ccccgccagt aacgtcgcca
    17041 tccgggctac gttacggttg tcttctcccg cctgattagc acagccgagg atcacatcat
    17101 cgatacactc cgcatcgaga cgcgggtttc gcaccagcag ctcccgcaaa gggatagcag
    17161 ccagatcgtc agcccgaaca cctgataatg ccccaccata gcggccaatt ggcgtacgaa
    17221 ttccgtcaca gataaaggct tcacgcatca ggcttctcct gtaatggtgc cgccgatgcg
    17281 gtgagattta ccgcgaaaca gcgcaaccgt tttttgttgt tggttaacaa tttcgatgtc
    17341 gtaaacaccg gtttgcttgc cctgatgacg tacctgcgca gtagcagtta aggtgtctcc
    17401 ggcaaagcct ggacgcaaaa aatcaatcgt gcaggcagaa gcgacggctg ccagcccctg
    17461 gctattgcag gcgtaggcaa aggcagtatc agccagtgaa aatagctgcc cgccgtggca
    17521 actttgatga ccgttaagca tttgtgcagt gacggtcatg gtcactacag caaagccttc
    17581 atccattgag ataatgtcga tgccaagcgc tttggcgcag gcatcgttct catacattgc
    17641 atgggcattt tgccaggcct tatgactcat aaccgctctc cagaagcgct cgttggcgca
    17701 gcaatgaaca tgggcgatag cgttcttcgc catagtgatg ttgtagattt tccaggagcc
    17761 ttaatattcg ctgccagcca agttgcgctc cccaggcaag cgggccatat ggataattca
    17821 ccccaagacg catggcggta tcgatatcct gttcagaggc cacgcctttt tgcagcgcat
    17881 caagggcttc attgatgatc attgctaccg ttcgccaaat cagcattcct gggtaatctg
    17941 caatttgcag cactgttttg ccctgctgtt gcaggtaata aatggccttg cgggtcgctg
    18001 agtccgggtt caccgctgcc gcagcaatgg tcaccacctt gcccgccatt ttgtcgacca
    18061 ctaccaccgg gcgtgccagt cgtattgcca gcgcctgtgc cgtctcgcct tgtgtctcaa
    18121 tcaataaaac atcgtcaatt tccgtgacgc cgtcactttt cttttctact ttcattgggc
    18181 taaaactgtc gcttaccgct tccaggccaa caactgcctc acgttccgcg cgccagtcgt
    18241 acacgcccag cccacttttc ttgcccaacc gtccaccaat caccagttcc tgttgcacca
    18301 gcgaaggtaa aaaacgacgc tcctgccaga aagcgttaaa caccgaacag gtgacagcaa
    18361 aattgacgtc ctgaccaatc agatcggtta attccagcgg ccccatcggg aaaccagcgc
    18421 catcgcgaag tgcagcgtca atcacttctg gtgcagcaac ctgctcttcc agtgcccgcc
    18481 aggcctcgga ataataagga cgcgccacac ggttaacgat aaatccaggg gtcgaatgac
    18541 agcgcacagg ctgcttaccc caactcaacg ttagttcaca caactgctca acaacttccg
    18601 ctgccgttgc cagcccactg accacctcca ccaacttcat caccggtgcc gggttaaaaa
    18661 aatgcagccc cgcaacacgt tcaggatttt ttacctccgc agcaatcgcg gttatagaga
    18721 ttgacgaagt gttagtggtc aatagcgttt gtggcgggca aacttccgcc agctgtgcaa
    18781 agagcgcttt tttgacttcc agacgttcag acgccgcttc aatgaccagg tccgcagctg
    18841 ccagcgcgtg aatatcggtc accgggatca ggcgtttcaa tgtgcgttca caggtttcag
    18901 cagtcagttt tccccgcgtc acgcgtgaat ttagccgcgc gtgtatcccg tcgattgcgc
    18961 gggtcagcgc ttcagcagaa atgtcataca gtaaaacctg atgtccatga ctggcggcaa
    19021 cttcagcaat gccagccccc atggtgccgc tcccaatcac tgccacagtt tgcacattta
    19081 tcatcatgct atttccccgt gaactgcggt gagcgtttag ccaggaacgc actgacacct
    19141 tcacgataat ctgcgctccg tccggcaagt cgctgatagt cgcgttccag atccagttgc
    19201 gtatcgagcg tattggtttc agcgctattt atcgcttgct tgataagtcc aagaccaaat
    19261 gtcggttgtg tcgccagatg ccgtgccagc tgttgcgcgg tatctgccag cgtttcatca
    19321 tcaacaacct gccagatcat cccccattcg tgcgcctgtt cagcactcag ttgattcccc
    19381 agcagtgcca gccccatggc gcgcgctcgc ccagcaacgc gtggcagtaa ccaggttcca
    19441 ccgcaatcgg gtattaagcc taacttacta aacgccatga cgaattttgc tgaacgggca
    19501 gcaataacga tgtcgcaccc cagtgccagt gttgcgcctg cccctgccgc cacgccattg
    19561 actgcacaga tcaccggttt tggcagtttt gccaggcgac gtaccagtgg gttatagaaa
    19621 cgttcaactg acatccctaa atccggtgcg gggcctgtgg gatcaacgtt acgatcgtta
    19681 agatcctgac cagcacaaaa tccgcgcccg gcacctgtaa gtaacaggca acggatagtg
    19741 tcgtcgcgct cgacctgttt caggcactct gccagttgtg cgtgcatctc atcattaaaa
    19801 ctgttcaggc gttccgggcg gttgagcgtt agtgtcatca cgcccttttc tacatgactg
    19861 aggatgaatt ccatcattag cgtcctttaa agtcgggcgt gcgtttttgt aagaaagcgg
    19921 agatgccttc atgacgatct tctgttgccg ccagcaaggt gaataactgt cgctcctggg
    19981 caagtccggc ttgcaaagcc acttcctgcg actggcgcag cgcttgcttt gccgcttgta
    20041 aggccaacgg cgagtgacgt gccattttcg atgccagctg taaggcgtat tcgagggtta
    20101 aatcgctggg gaagacgtcg ctaaccagcc cggcctgctg tgcttgctgg gcggtgatac
    20161 tttctccgct cagcaccatt ttgctggcta acgatttacc gacactacgg attaaacgtt
    20221 gcgttccgcc tgcgccaggc atgatgccga gagtgatttc cggcaaccca aaacgcgcgt
    20281 tctctccggc aaccaccaca tcgcacaaca atgccagttc gcaacccgcc ccaagcgcgt
    20341 aaccattgac ggctgcgata agtggtttat taaaggcctg caatcgcgcc catagctgcg
    20401 gacgtgtatc gtttaaggtg gccgcgagat ctttttctgc catttcgttg agatcggccc
    20461 cagcggcaaa aaagcgtgca ttaccggtaa tcacacagac cgaaatgctg gtatcggtag
    20521 ccgcagcttc cagttcattt accagttgca tcagcagggc attatttagc gcattacgtg
    20581 cggcgggacg gttaagggtc agcaacaata ctcgttgctg acggctgacg atcagttcgc
    20641 tcatgccatc cccttcgcgt caaagtcaac caccacatcg ctggtcagcg gcagtgcctg
    20701 gcaactcaac acataacctg cggccagttc atccggttcc agactgtaat tggtttccat
    20761 cgccactttg ccacgcagca ctttgcattt gcaggtcgca cagacgccgc ctttgcaggc
    20821 atagggcaga tcagccccct ggcgcaatgc cgcatcgaga atgctttcat cgtcggcatt
    20881 aagcacgatt tcccgatccc gcccatcctg acgtacagtc actttttgtc cgtcactttg
    20941 cacgttaacg ctacgtttga cgcgcgtgcc aggcgtatta aaccgctcca gatgaatggt
    21001 tttatctggc attcccagtg cttttaaggc ggcttccgtc tcatccatca tcgccgccgg
    21061 accacaaata aatgcctcat cataaagacg aaaattaatg agcgaggccc caagtgactg
    21121 taatctttca ccgtcaatac gcccgtgaag cagatcgctg tcgagggttt cctgactgaa
    21181 aatgcacaac aactgtaaac gctgaggata tttgtctttc aggtctgcca gtgcctggcg
    21241 aaacatcatg ctctggctgg tacggttacc gtagatcagg gtgaactgac tttcaggctc
    21301 ggtttgtaaa gtggtggcga taatcgccag cattggcgta atacctgatc ctgctgcaat
    21361 agccagatag cggccctggc gttcggcctg gggctgatag ccgaaatgcc cctgcggcac
    21421 catgacctcc agcgtcatac cctggcggat gtgttcgcgg gcatagcggg agaaacgtcc
    21481 gccttcaatg gctttcaccg ccacactaat ttcgccaggc aggtagctgc ggcaaatgga
    21541 gtaacaacgg cgtaattctt caccatcaag actggctttt aaggtcaaat gttgaccggg
    21601 gcgaaagcga tacgcctcct gcaaaggctg gggcaccgca aaggtaatgg tcaccgcatc
    21661 acgggtttcc ggctcgactt ttgccactgt taaggaatga aacgttgtca tggcatcctc
    21721 aaatacattt gaaataatcg aaaggttcgc ggcaactatc gcagcggtac aatgctttgc
    21781 aggccgtgga accaaattca ctgataagtg tggtatggac gctggcgcag cgcgggcaac
    21841 gtacttctgg cggcaaatgg gcatggcaac tgtgtccggc gggcgggcta atgccatact
    21901 gtcgcagacg ctcacgggca tcgggggtca tccagtcggt ggtccatgcc gggtcgagtt
    21961 gcagcacaac ctgaacgggg gtaaacccgt gggttgtcat tgcctcacgt atcgcgccaa
    22021 tcaaatgttc cgttgccgga caaccggaat aagtcggcgt aaagccgatc acccatcctt
    22081 ctcccatctg tgtcacatta cgcaccatgc ctaaatcggt aatggtcagc accgggatct
    22141 ccgggtccgg gatctggctg agtagcgccc atatctcatg aacctgtggc ggtgcaatgg
    22201 tggccagacg ttgcataccc atctcctctg ttaccattgc tgaccgggca agacacgctg
    22261 gagatactgc atttctgcca gcatgggtcc aaggtgttcg gtatgcagtc cttttttacc
    22321 gccagtgcga tacgcctgct cttgcggtac gttcaatgtg gcttcgttga ttccggcaaa
    22381 aacttcggct tcccacgctg cgcgtaaagt gcgtggatca accgcaatac cctcttcact
    22441 cagtgcaata tcaatctcgt cggcatcgaa cagttcggca gtaaaacgcc acaacttgtc
    22501 gatggcctgc tgcatctttt gccctgatac gtcagtacca ttgcccagtc gctccagcca
    22561 gccacgacta aaacgcaggt gataacgcgc ttctttaatt gctttggcag aaatcgccgc
    22621 cagttgcgga tcacggcttt ccatcagacg ggtaaagagc gccacatgcc atgcatcgat
    22681 gaaatactgg cgtgcaatgg tgtcggcaaa attgccgttt ggttgttcaa ccaacaataa
    22741 gttgctgaac tggcgctcgt ctcgggtgaa ggccagggta tcttcatcac cttctcccgc
    22801 taattcagcg gcatacgata agaagttacg tgcctgacct aataaatcga ggccaatgtt
    22861 tgccagtgcg agatcgattt ccagttccgg tgcgtgaccg caccattccc ccaaccgctg
    22921 ggagagcacc agatagttat cgcccaggcg caaggtgtaa gccgttaact gattcatttc
    22981 cgacctcaca tgtgctcaat gccatcaggg atggtgtaaa acgttggatg gcgatagacc
    23041 ttgctttcag ccggatcaaa aaattcaccg cgttcttccg gttgcgaggc aacaatttca
    23101 ctcgccttca ccacccaaat tgaacatcct tcgctacgac gggtgtaagc atcacgggca
    23161 ttttccagtg ccatccgctc atcggcagca tgtaaactgc caacatggcg gtgtgataag
    23221 ccctgtttgc cacgcacgaa aacttcgtat aacggccagt aaacattact cattttgaat
    23281 ctccttatgc gaccttacgg gcaagttgtt tttgtgcatg ggccagcgct gcttcccgta
    23341 cccaggtacc ttcttcccag gctttacgtt tggcgtcgag cctctcctga ttacaaattc
    23401 cgcgaccgtt aattacttcg ttaaactcct gccagtcgat ctcaccaaag cggtagtgac
    23461 cgctttcagt gtcaaaatgc agatccgggt caggaacggt cataccgagc atttcaacct
    23521 gtggaacggt gttgtccacg aaacgctggc ggagttcgtc gttggtgaaa cgtttgattt
    23581 tccaggcgag acttctggcg ctgtttggcg agttatcatc gtttggcccg aacatcatta
    23641 aggctggcca ccagaaacgg ttaatggcgt cttgcaacat ctgcttttga gcttcgctgc
    23701 cctgcgccag tgccatgcag gcttcaaacc cctgacgctg gtgaaaactc tcttctttgc
    23761 agattttcac cattgctctg gcatacgggc cataagaagt tcggcataac gccacctgat
    23821 tgacgatcgc tgcgccatcc accagccagc cgataacgcc gatatcagcc caactcaatg
    23881 tcggataatt aaagatggag gaatatttca tccgtccgtc gagcattttt tggtagatgt
    23941 cttctctggc acagcccagt gtttcggcgg cgctatagag atacagacca tgaccggctt
    24001 catcctgcac tttggctaac agaatggctt tacgccgcaa ggttggtgcg cgagtgatcc
    24061 agttaccttc aggcaacata ccaacaatct cggagtgcgc atgctgccca atctggcgga
    24121 tcaatgtctt tcggtaagca tcgggcatcc agtcctgcgg ctcgatagcc gtttcctggg
    24181 ctatccgttg ctcaaagcgt tcttcttggg tcacgtcatc acctttacga ttccagtttt
    24241 cgtgaattaa ttaaaaacac gaatcacgtt gttttatgaa agttacacaa tggttaacta
    24301 aagtcacaaa ttttgtatac ttgaactgtg acagatttcg cactctattt tgataactca
    24361 ttatttatca gtgcattaat gtttttagta gttgatatac agatcacgca gttaacattt
    24421 cgttaaaaga tcccttgctt tttatgattc gcgatttaac tattagcaac agaaatgtga
    24481 aacatctgga gagtagcgat gcagcagtta gccagtttct tatccggtac ctggcagtct
    24541 ggccggggcc gtagccgttt gattcaccac gctattagcg gcgaggcgtt atgggaagtg
    24601 accagtgaag gtcttgatat ggcggctgcc cgccagtttg ccattgaaaa aggtgccccc
    24661 gccctccgcg cgatgacctt tatcgaacgt gcggcgatgc ttaaagcggt cgctaaacat
    24721 ctgctgagtg aaaaagagcg tttctatgct ctttctgcgc aaacaggcgc aacgcgggca
    24781 gacagttggg ttgatattga aggtggcatt gggacgttat ttacttacgc cagcctcggt
    24841 agccgggagc tgcctgacga tacgctgtgg ccggaagatg aattgatccc cttatcgaaa
    24901 gaaggtggat ttgccgcgcg ccatgtactg acctcgaagt caggcgtggc agtgcatatt
    24961 aacgccttta acttcccctg ctggggaatg ctggaaaagc tggcaccaac gtggctgggc
    25021 ggaatgccag ccatcatcaa accagctacc gcgacggccc aactgactca ggcgatggtg
    25081 aaatcaattg tcgatagtgg tcttgttccc gaaggcgcaa ttagtctgat ctgcggtagt
    25141 gctggcgact tgttggatca tctggacagc caggatgtgg tgactttcac ggggtcagcg
    25201 gcgaccggac agatgctgcg agttcagcca aatatcgtcg ccaaatctat ccccttcact
    25261 atggaagctg attccctgaa ctgctgcgta ctgggcgaag atgtcacccc ggatcaaccg
    25321 gagtttgcgc tgtttattcg tgaagttgtg cgtgagatga ccacaaaagc cgggcaaaaa
    25381 tgtacggcaa tccggcggat tattgtgccg caggcattgg ttaatgctgt cagtgatgct
    25441 ctggttgcgc gattacagaa agtcgtggtc ggtgatcctg ctcaggaagg cgtgaaaatg
    25501 ggcgcactgg taaatgctga gcagcgtgcc gatgtgcagg aaaaagtgaa catattgctg
    25561 gctgcaggat gcgagattcg cctcggtggt caggcggatt tatctgctgc gggtgccttc
    25621 ttcccgccaa ccttattgta ctgtccgcag ccggatgaaa caccggcggt acatgcaaca
    25681 gaagcctttg gccctgtcgc aacgctaatg ccagcacaaa accagcaaca tgctctgcaa
    25741 ctggcttgtg caggcggcgg tagccttgcg ggaacgctgg tgacggctga tccgcaaatt
    25801 gcgcgtcagt ttattgccga cgcggcacgt acgcatgggc gaattcagat cctcaatgaa
    25861 gagtcggcaa aagaatccac cgggcatggc tccccactgc cacaactggt acatggtggg
    25921 cctggtcgcg caggaggcgg tgaagaatta ggtggtttac gagcggtgaa acattacatg
    25981 cagcgaaccg ctgttcaggg tagtccgacg atgcttgcag ctatcagtaa acagtgggtg
    26041 cgcggtgcga aagtcgaaga agatcgtatt catccgttcc gcaaatattt tgaggagcta
    26101 caaccaggcg acagcctgtt gactccccgc cgcacaatga cagaggccga tattgttaac
    26161 tttgcttgcc tcagcggcga tcatttctat gcacatatgg ataagattgc tgctgccgaa
    26221 tctattttcg gtgagcgggt ggtgcatggg tattttgtgc tttctgcggc tgcgggtctg
    26281 tttgtcgatg ccggtgttgg tccggtcatt gctaactacg ggctggaaag cttgcgtttt
    26341 atcgaacccg taaagccagg cgataccatc caggtgcgtc tcacctgtaa gcgcaagacg
    26401 ctgaaaaaac agcgtagcgc agaagaaaaa ccaacaggtg tggtggaatg ggcggtagag
    26461 gtattcaatc agcatcaaac cccggtggcg ctgtattcaa ttctgacgct ggtggccagg
    26521 cagcacggtg attttgtcga ttaatcggtg aatgaagggc aacggccaat agttgccctt
    26581 ttatttcact aagttttgtg acgttgtcac attatgcatg atgtgtacat ctattttcag
    26641 ggcacccact gtatgaaaag ctggcacacc tgccaaacaa cctggcaggt gcaggcaatc
    26701 ccctttgcat cagtactgat aatgtgaacc tgactaaacc gcccacagag cgcggttgct
    26761 aacaagaaca caacatctga cgaggttaat aatgggaagc ccctctctgt attctgcccg
    26821 taaaacaacc ctggcgttgg cagtcgcctt aagtttcgcc tggcaagcgc cggtatttgc
    26881 ccacggtggt gaagcgcata tggtgccaat ggatgaaacg cttaaagaat ttggtgccga
    26941 tgtgcagtgg gacgactacg cccagatctt taccctgatt aaagatggcg cgtacgtgaa
    27001 agtgaagcct ggtgcgcaaa cagcaattgt taatggtcaa cctctggcac tgcaagtgcc
    27061 ggtagtgatg aaagacaata aggcctgggt ttctgacacc tttattaacg atgttttcca
    27121 gtccggtctg gatcaaacct ttcaggtaga aaagcgccct cacccactta atgcgctaac
    27181 tgcggacgaa attaaacagg ccgttgaaat tgttaaagct tccgcggact tcaaacccaa
    27241 tacccgtttt actgagatct ccctgctacc gccagataaa gaagctgtct gggcgtttgc
    27301 gctggaaaac aaaccggttg accagccacg caaagccgac gtcattatgc tcgacggcaa
    27361 acatatcatc gaagcggtgg tggatctgca aaacaacaaa ctgctctcct ggcaacccat
    27421 taaagacgcc cacggtatgg tgttgctgga tgatttcgcc agtgtgcaga acattattaa
    27481 caacagtgaa gaatttgccg ctgccgtgaa gaaacgcggt attactgatg cgaaaaaagt
    27541 gattaccacg ccgctgaccg taggttattt cgatggtaaa gatggcctga aacaagatgc
    27601 ccggttgctc aaagtcatca gctatcttga tgtcggtgat ggcaactact gggcacatcc
    27661 catcgaaaac ctggtggcgg tcgttgattt agaacagaaa aaaatcgtta agattgaaga
    27721 aggtccggta gttccggtgc caatgaccgc acgcccattt gatggccgtg accgcgttgc
    27781 tccggcagtt aagcctatgc aaatcattga gcctgaaggt aaaaattaca ccattactgg
    27841 cgatatgatt cactggcgga actgggattt tcacctcagc atgaactctc gcgtcgggcc
    27901 gatgatctcc accgtgactt ataacgacaa tggcaccaaa cgcaaagtca tgtacgaagg
    27961 ttctctcggc ggcatgattg tgccttacgg tgatcctgat attggctggt actttaaagc
    28021 gtatctggac tctggtgact acggtatggg cacgctaacc tcaccaattg ctcgtggtaa
    28081 agatgccccg tctaacgcag tgctccttaa tgaaaccatc gccgactaca ctggcgtgcc
    28141 gatggagatc cctcgcgcta tcgcggtatt tgaacgttat gccgggcctg agtataagca
    28201 tcaggaaatg ggccagccca acgtcagtac cgaacgccgg gagttagtgg tgcgctggat
    28261 cagtacagtg ggtaactatg actacatttt tgactggatc ttccatgaaa acggcactat
    28321 tggcattgat gccggtgcta cgggcatcga agcggtgaaa ggtgttaaag cgaaaaccat
    28381 gcacgatgag acggcgaaag atgacacgcg ctacggcacg cttatcgatc acaatatcgt
    28441 gggtactaca caccaacata tttataattt ccgcctcgat ctggatgtag atggcgagaa
    28501 taacagcctg gtggcgatgg acccagtggt aaaaccgaat actgccggtg gcccacgcac
    28561 cagtaccatg caagttaatc agtacaacat cggcaatgaa caggatgccg cgcagaagtt
    28621 tgatccgggc acgattcgtc tgttgagtaa cccgaacaaa gagaaccgca tgggcaatcc
    28681 agtttcctat caaattattc cttatgcagg tggtactcac ccggtagcaa aaggtgccca
    28741 gttcgcgccg gacgagtgga tctatcatcg tttaagcttt atggacaagc agctttgggt
    28801 aacgcgttat catcctggcg agcgtttccc ggaaggcaaa tatccgaacc gttctactca
    28861 tgacaccggt cttggacaat acagtaaaga taacgagtcg ctggacaaca ccgacgccgt
    28921 tgtctggatg accaccggca ccacacatgt ggcccgcgcc gaagagtggc cgattatgcc
    28981 gaccgaatgg gtacatactc tgcttaaacc atggaacttc tttgacgaaa cgccaacgct
    29041 aggggcgctg aagaaagata agtgattgtt tcagacaaaa aaacggcacc aggtgccgtt
    29101 ttttacttat gagcgaacca gattaatacc gtacacacac cgacttagtt tcacaccaac
    29161 cgtccagcca gtcggggcca aaatcacggc ccgttcctga ctgcttcatc ccaccaaacg
    29221 gtaagttagc gtcaattaag gtatggctgt ttacccacac cgtccctgcc tgtaagcgat
    29281 cgctatattc cagagcctgg gagagatttt gcgtccagac actggcagtt aagccatatt
    29341 ccgtgtcgtt tgccagttgt aacgcctctt ctccatccgc tactcgcacc aggtttacca
    29401 ccggaccaaa cacctcttca cgagttaagc gcaatttagc atcgggattt accaccagcg
    29461 ttggcgcaac ataatacccc tctccggctg gtccattcga cccgcgaatc agctctgctt
    29521 gctgtgcctg cgcatcgtcg aggaatgaac acactttgtc gcagtgcgca cgagaaacca
    29581 aagggttaat ctgtgcaaca ggtgacatcc ccggtcccac ttgcaacgat tttaccgcct
    29641 gctcaaatcc actaaccagc gtgtcaaaca acggcgcttc aatataaatt cgcgaactgg
    29701 cggcgcatac ttgcccttga ttcaggaagc ttccggtcat caagccttca ataacccatt
    29761 gcggatcagc atcttttaat acaattgccg ggtttttacc gcccagttcc agcgttacac
    29821 gcgttaagtg atcagcagca gttctggcaa taccttttcc cgtcgcggtt gaaccggtaa
    29881 aactgatttt cgcaacatga ggatgtgacg tcagggccgc gccgcataca gcacctgacc
    29941 cggtgacgac attaaaaacg ccatcaggga taccagcctc gctggccagt tccgccacgc
    30001 gcaacatcgt cagtggcgtg gtttccgaag gcttaatcac gattgaacag cctgctgcca
    30061 gtgctggcat caccttccac ataccaatca tcaacggaaa gttccatggc acaattcccg
    30121 ccactacgcc aaccggctct ttacgcgtcc aggcctgata acgcgccccc tggggtaagg
    30181 gaatcgacaa gtccagcgtt ttacccgcga ttttggtcgt taacccggcg gtataacgca
    30241 tccagttcag cgtacagccc acttcaaaag cacgagaaat ggcaattgac ttgccttgct
    30301 ccagggtttc cagttgcgcc agctcctcac tgtgctgctc caccagatca gcaaaacgta
    30361 gcagaatacg ttcacgctct gcgggtaatc gcccggccca gcgacgcgag acaaaggccc
    30421 gccaggcaga catgactgcg ttatctacat ccgcttcgtt ggcatcagca gtagacgcaa
    30481 tttcttgccc ggtggccgga tcaaagatcg ccaatcgttt ttcactttgt gcggggcctg
    30541 gacgaccatc aatataaaga ccgtgttgac gatcgagaaa ctgttggacc tggcttaata
    30601 ctgctacatg cggctctgtc atcacttttc cttattattt acccagtgtg atttcagtgt
    30661 acgcagcgac aagaaaagtc acttttttcg ctgtgacatt cgctttgtca gctgtgacaa
    30721 gctccgcaaa tcgtgacaat aaccgcttat gtctctatgc ctgagaagac ttatttccta
    30781 tagtcaggct cattagcttc gtttactgca acacaaatgc aacaataaaa atacatttca
    30841 cagagcgaaa acgtgccatg aaccccgcag tggataatga gtttcagcaa tggctttccc
    30901 aaatcaatca ggtatgcgga aattttaccg gacacctgct gactgagcgt tacacgggtg
    30961 tactggatac gcattttgcc aaaggactaa agctgagtac cgtgacaacc agcggggtga
    31021 atttatcccg cacctggcag gaagtaaaag gcagcgacga tgcctggttt tacaccgttt
    31081 ttcagcttag tggtcaggca ataatggagc aggatgagcg tcaggtgcag attggcgctg
    31141 gcgatattac gttactcgat gcctcacgcc cctgttcgct ttactggcag gagtcttcta
    31201 aacagatttc attacttttg ccacgcactc tgctggaaca atattttccc catcaaaaac
    31261 ctgtctgcgc agaaagactg gacgctgact tacccatggt gcaactcagt catcgcctgt
    31321 tacaggagag catgaataat ccggcacttt ctgaaacaga aagtgaagct gcgctacagg
    31381 cgatggtgtg tctgctgcgc ccggtacttc atcagcggga atctgttcaa cctcgtcgtg
    31441 aacgtcagtt tcaaaaagtg gttacgttga tagacgataa tattcgcgaa gagatattac
    31501 gcccggagtg gatagccgga gagacaggta tgtcagtacg tagtttgtac cgaatgtttg
    31561 ccgataaagg tttggtagtc gcgcaatata ttcgtaaccg tcgtctcgat ttttgtgcag
    31621 atgcgattcg ccatgccgca gatgatgaaa aactggcagg catcggcttt cattggggat
    31681 tttctgacca gagtcatttt tcaacggtat ttaagcaacg ctttgggatg acgccaggcg
    31741 agtatcgacg taaattccgc taactaattg gcgttgtgtg tactgcaacg ccaattagtt
    31801 aaaacagata tttgactatg catttttcag ggcgaaaggc aatttgacag gagtttttga
    31861 atattcacct tgtttcagga aaatagatca atcgctttct cctgtagcgt tatcccgata
    31921 ttgcggtttt gctattaaaa ttttcgcacc catttaccgt ttatcccttg tacatattcc
    31981 cccggtttgg cccgagctac gagcttttgc ccggcgagtt tcgcgatatc atctacagac
    32041 acattatttt gctttgccag ttgttggtaa ctcgctttac gttcggcatt aatgtcggtt
    32101 actaatttct ctgtttcagc atccgttttc agcgcaacca gataaccata aaatgtttca
    32161 cctacccgcc cctgagttct ggcttcatcc agagtcaatg ccattacatt gctgcttacc
    32221 agcccaacaa gaaacgcaca aaaaagtaat gttttcttca tcatcgcctc agaaaagatc
    32281 gctacgagtt tcaagcagct cttcgacatc tttgtctgcc ttgatgatga tctcatgctc
    32341 aattttaacg ttcatattga tagtgatcgg ttccttgggc gcagcgactt caatacgcgg
    32401 cgtacaggca gccagcataa aagatgacgt caacgcagcc agtaaaattt tcattgtttt
    32461 tcctcacact cttttccttg cggacagtca tttcccggca gacgtgcgtt ctgctccaac
    32521 catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat attctcttca
    32581 tgatggtaat taaggtttac cgtaccgctt ttaccatcaa cgcgactggt acctgtaatg
    32641 ttggcctgca tggttaataa accgagatta tctaagttaa tttttgtcga tgaccggcta
    32701 atttccatat agcgcaacca gttaattgct gaacccgcag tcatattgtc tttcaccacc
    32761 gcatctgcgg tgtctttatc cagccgcaag gtcattggcc cgctatttgc cagccaacca
    32821 tctttcacta tccatttttc gctgtttagc cataacggca atgcaccact aaatgccccg
    32881 gacatggcga attgtttcgg attgacggcg ctaaccagtt cgctggatga gagattattc
    32941 agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag tacgttaccg
    33001 cctaacacat cgacgctaac atcagtcaac agcaagggtt cttcttcagt ccacggataa
    33061 cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac ttcggcaatt
    33121 cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa acggaaaggc
    33181 aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc gcctttcaac
    33241 acgccgtgtc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc aacctgagca
    33301 tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg ctggaataca
    33361 gtcagtgatt gttttggcca ccaggcgtta ccgcgcagac gaataccgtc ccagcgacca
    33421 tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag gaaatacgtc
    33481 ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact gccgccagtg
    33541 aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg ctgtgcgtca
    33601 cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt catcgtaccg
    33661 tattgtaact ggtcgaaacc ggtggagagg tcggtcagcg taatcgtgct gtcatgccat
    33721 tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactgcc ttttccccag
    33781 tagcgccact gccagcggcc cgcgtcaggg agaaaatcat tcgccagccc atccatatgc
    33841 agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcaa acgtccgtca
    33901 acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc gatgtccagc
    33961 gaatcgatga ctcttccctt cgaacgtaat aacgcgcctg gctcaaaggc cagcgttggg
    34021 tcagtcagac ttccacttag ctgcgcgggt aagcgggcat ataaaatgag gtccgcctgt
    34081 ttcgcttcac cggtcagttg cagaggcagc tggctgttat ccatgcttaa ttttcctggg
    34141 ccaaaattta gcaccgcgtt gcctttaccc gcttgcccct gggtcagcac actcagtcgt
    34201 ccgctgatca gagcgttctc aagccctgcc tgccagttgt cgactttgac acccagtcgg
    34261 ccactcaaag gaaaacctgc atacggccaa ctccagcgac catcgcttac ggtcaattgt
    34321 tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg tgccagcaca
    34381 atcaattgcc cgctattttc ctgccagtcc agctctgcat ccaccagtga cggttcctgc
    34441 ggcaagttta acgtcgcagt agcatgccca cttacaggaa gtccatccgg cacgagcggc
    34501 atagtaaatt cccccaccag tttaaccggc ggctgatttt caaacgcgac gacatccagt
    34561 tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt ttcgccctga
    34621 taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg ccattcctgc
    34681 cagggagaaa aaatcagttt atcgatattg atccaggtgt taggcagcat ggactgccac
    34741 tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt cgccagacaa
    34801 gcagaatcaa gttctaccgt gccgacgttc aataaccagc ggctgggatg tgaaaggctg
    34861 gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc gggaatgatt
    34921 aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc gagcggtagc
    34981 cagatgcccg ccagagtggg aacccacagc ccgagcgtca tcaacagcgt caacggcaca
    35041 agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat gggtagttaa
    35101 tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg tttaaccgtt
    35161 cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa tatcgccata
    35221 gctttcaatt aaatttgaaa ttttgtaaaa tatttttagt ggcttaaatg tgattcaaca
    35281 tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt
    35341 acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga
    35401 cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg
    35461 acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc
    35521 gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag
    35581 tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga
    35641 cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac ttctctctgg
    35701 aaggtctgac cggatttact atgtatggca aaacggcagg cgttatcggt accggtaaaa
    35761 tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc
    35821 cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt
    35881 tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt
    35941 tgaacgaagc cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg
    36001 gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attggttcgt
    36061 tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagacaaa tccaacgacg
    36121 tgatccagga tgacgtgttc cgtcgtctgt ctgcctgcca caacgtgctg tttaccgggc
    36181 accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact
    36241 taagcaatct ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc
    36301 ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct ctattcttaa
    36361 aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaactag ccgcgtttgt
    36421 tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg ttacgccaga
    36481 acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga
    36541 taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa
    36601 ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat
    36661 gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat
    36721 gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa
    36781 acagacatta acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc
    36841 ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt
    36901 accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc
    36961 actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg cctgctgaac
    37021 aggttcacta ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg
    37081 catctcttct tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca
    37141 gagggcggcg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat
    37201 ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga
    37261 aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc gcagcaattt
    37321 cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc
    37381 gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc
    37441 agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg
    37501 ggagacacac cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat
    37561 ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg
    37621 atgatcccga cgctgtacaa actggcaggc gaactcacac cgtttgtcct gcatgtagcg
    37681 gcacgtaccg ttgccacaca tgcactctct atttttggcg atcattccga cgtcatggcg
    37741 gtgcgccaga cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc gcaagacttt
    37801 gctctcattt cgcacatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat
    37861 ggtttccgca cgtcccacga aatcaacaaa attgtcccgc tggccgatga cacgattctt
    37921 gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcactcaa cccggaacat
    37981 ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg cgaagccacc
    38041 aacccatggt acgacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc
    38101 gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg
    38161 attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc
    38221 cgtggcgaaa aagtcggcgt gctgaaagtt cgcctgtacc gccccttctc agccaaacac
    38281 ttgctacaaa ctctaccggg atccgtacgt agcgtggcgg tactggacag aaccaaagaa
    38341 cccggtgccc aggcagaacc gctctatctg gatgtgatga ccgcactggc agaagccttt
    38401 aataatggca agcgcgaaac tctcccccgt gtcattggtg ggcgctatgg tctttcatcc
    38461 aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg
    38521 aaagcgcgct ttacggttgg tatttacgat gatgtaacca atctgtccct gccgttgccg
    38581 gaaaacaccc agccaaactc ggcgaaactt gaagccttgt tttatggcct tggtagtgat
    38641 ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac
    38701 gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt
    38761 cgagtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc
    38821 tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc
    38881 attttcctgc tcaacacacc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaagaa
    38941 gttcaggccg tgttaaacca gaaaaaagcg cgcttctatg tgattaacgc ggcgaaaatc
    39001 gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat
    39061 ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa
    39121 agttacagta gcaaaggcca ggatctggtg gaacgcaatt ggcaggctct ggcgctggcg
    39181 cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg
    39241 ccagtggttt ccgatgccgc tcctgatttc gtgaaaaccg taaccgctgc gatgctcgcc
    39301 gggcttggtg acgccctccc cgtttcggcg ctgccgccag acggctcctg gccgatgggc
    39361 actacgcgct gggaaaaacg taatattgcc gaagagatcc ccacctggaa agaggaactc
    39421 tgtacccaat gtaaccactg cgttgccgct tgcccacact cagctattcg cgcaaaagtg
    39481 gtgccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg
    39541 cgtgatatgc gcgggcagaa atatgtcttg caggtggcac cggaagattg caccggttgt
    39601 aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc
    39661 aatatgatgt ctcgcctgga acatgtcgaa gaagagaaaa tcaattacga tttcttcctc
    39721 aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg
    39781 attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac gccgtatatt
    39841 aaattgctga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac cggctgttct
    39901 tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa cggtcgtggg
    39961 ccggcatggg cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg
    40021 acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc
    40081 ccggcggaat tactgacggc gttgaaatca gacgctacgc cagaggttcg tcgagaacag
    40141 gtcgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact gctgcgtgac
    40201 gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg ggcttacgat
    40261 atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg
    40321 ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt
    40381 gcagtaacta agttcggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt
    40441 atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca gctgaaccag
    40501 acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctgat cattgcttat
    40561 agcccgtgtg aagagcatgg ttacgatctg gcactcagcc atgaccagat gcgccaactc
    40621 acagctaccg gcttctggcc gctatatcgc ttcgatcctc gtcgtgccga tgaaggcaaa
    40681 ctgccgctgg cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac
    40741 gagcaacgtt tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa
    40801 gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg
    40861 gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta aaaaaaaagc
    40921 ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca atgcaatggc
    40981 ccaacagaaa ataaaatctg tgacaataaa ggcatataac ccgcgcagaa taacgtatag
    41041 aaaattaaaa gtataatttt tatt
//
LOCUS       DAZKGS010000021        40731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKGS010000021 DAZKGS010000000
VERSION     DAZKGS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            176..871
                     /gene="aqpZ"
                     /locus_tag="ACHOQY_001174"
     CDS             176..871
                     /gene="aqpZ"
                     /locus_tag="ACHOQY_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9677315.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1366..2265
                     /gene="lysO"
                     /locus_tag="ACHOQY_001175"
     CDS             1366..2265
                     /gene="lysO"
                     /locus_tag="ACHOQY_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9677316.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            2409..4061
                     /gene="hcp"
                     /locus_tag="ACHOQY_001176"
                     /gene_synonym="priS"
     CDS             2409..4061
                     /gene="hcp"
                     /locus_tag="ACHOQY_001176"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9677317.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            4073..5041
                     /gene="hcr"
                     /locus_tag="ACHOQY_001177"
     CDS             4073..5041
                     /gene="hcr"
                     /locus_tag="ACHOQY_001177"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9677318.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            5174..6892
                     /gene="poxB"
                     /locus_tag="ACHOQY_001178"
     CDS             5174..6892
                     /gene="poxB"
                     /locus_tag="ACHOQY_001178"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9677319.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6929..7930
                     /gene="ltaE"
                     /locus_tag="ACHOQY_001179"
     CDS             6929..7930
                     /gene="ltaE"
                     /locus_tag="ACHOQY_001179"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9677320.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7941..9371
                     /gene="ybjT"
                     /locus_tag="ACHOQY_001180"
     CDS             7941..9371
                     /gene="ybjT"
                     /locus_tag="ACHOQY_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9677321.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            9470..10483
                     /gene="ybjS"
                     /locus_tag="ACHOQY_001181"
     CDS             9470..10483
                     /gene="ybjS"
                     /locus_tag="ACHOQY_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9677322.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(10480..11310)
                     /gene="amiD"
                     /locus_tag="ACHOQY_001182"
     CDS             complement(10480..11310)
                     /gene="amiD"
                     /locus_tag="ACHOQY_001182"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9677323.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(11307..11630)
                     /gene="ybjQ"
                     /locus_tag="ACHOQY_001183"
     CDS             complement(11307..11630)
                     /gene="ybjQ"
                     /locus_tag="ACHOQY_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9677324.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            11756..12271
                     /gene="ybjP"
                     /locus_tag="ACHOQY_001184"
     CDS             11756..12271
                     /gene="ybjP"
                     /locus_tag="ACHOQY_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9677325.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            12489..13217
                     /gene="artP"
                     /locus_tag="ACHOQY_001185"
     CDS             12489..13217
                     /gene="artP"
                     /locus_tag="ACHOQY_001185"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9677326.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            13235..13966
                     /gene="artI"
                     /locus_tag="ACHOQY_001186"
     CDS             13235..13966
                     /gene="artI"
                     /locus_tag="ACHOQY_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9677327.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            13973..14689
                     /gene="artQ"
                     /locus_tag="ACHOQY_001187"
     CDS             13973..14689
                     /gene="artQ"
                     /locus_tag="ACHOQY_001187"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9677328.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            14689..15357
                     /gene="artM"
                     /locus_tag="ACHOQY_001188"
     CDS             14689..15357
                     /gene="artM"
                     /locus_tag="ACHOQY_001188"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9677329.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            15649..16380
                     /gene="artJ"
                     /locus_tag="ACHOQY_001189"
     CDS             15649..16380
                     /gene="artJ"
                     /locus_tag="ACHOQY_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9677330.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEMV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(16555..17682)
                     /gene="rlmC"
                     /locus_tag="ACHOQY_001190"
     CDS             complement(16555..17682)
                     /gene="rlmC"
                     /locus_tag="ACHOQY_001190"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9677331.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17723..18211)
                     /gene="ybjO"
                     /locus_tag="ACHOQY_001191"
     CDS             complement(17723..18211)
                     /gene="ybjO"
                     /locus_tag="ACHOQY_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9677332.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(18271..19116)
                     /gene="potI"
                     /locus_tag="ACHOQY_001192"
     CDS             complement(18271..19116)
                     /gene="potI"
                     /locus_tag="ACHOQY_001192"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9677333.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(19113..20066)
                     /gene="potH"
                     /locus_tag="ACHOQY_001193"
     CDS             complement(19113..20066)
                     /gene="potH"
                     /locus_tag="ACHOQY_001193"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9677334.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(20076..21209)
                     /gene="potG"
                     /locus_tag="ACHOQY_001194"
     CDS             complement(20076..21209)
                     /gene="potG"
                     /locus_tag="ACHOQY_001194"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9677335.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(21304..22416)
                     /gene="potF"
                     /locus_tag="ACHOQY_001195"
     CDS             complement(21304..22416)
                     /gene="potF"
                     /locus_tag="ACHOQY_001195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9677336.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(22767..23243)
                     /gene="ybjN"
                     /locus_tag="ACHOQY_001196"
     CDS             complement(22767..23243)
                     /gene="ybjN"
                     /locus_tag="ACHOQY_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9677337.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(23331..24233)
                     /gene="rimK"
                     /locus_tag="ACHOQY_001197"
     CDS             complement(23331..24233)
                     /gene="rimK"
                     /locus_tag="ACHOQY_001197"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9677338.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(24294..25016)
                     /gene="nfsA"
                     /locus_tag="ACHOQY_001198"
     CDS             complement(24294..25016)
                     /gene="nfsA"
                     /locus_tag="ACHOQY_001198"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9677339.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(25000..25287)
                     /gene="ybjC"
                     /locus_tag="ACHOQY_001199"
     CDS             complement(25000..25287)
                     /gene="ybjC"
                     /locus_tag="ACHOQY_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9677340.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            25447..25704
                     /gene="grxA"
                     /locus_tag="ACHOQY_001200"
     CDS             25447..25704
                     /gene="grxA"
                     /locus_tag="ACHOQY_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9677341.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(25734..26111)
                     /gene="ybjM"
                     /locus_tag="ACHOQY_001201"
     CDS             complement(25734..26111)
                     /gene="ybjM"
                     /locus_tag="ACHOQY_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9677342.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            26381..28066
                     /gene="ybjL"
                     /locus_tag="ACHOQY_001202"
     CDS             26381..28066
                     /gene="ybjL"
                     /locus_tag="ACHOQY_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9677343.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(28241..28777)
                     /gene="rcdA"
                     /locus_tag="ACHOQY_001203"
     CDS             complement(28241..28777)
                     /gene="rcdA"
                     /locus_tag="ACHOQY_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9677344.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            28861..30069
                     /gene="ybjJ"
                     /locus_tag="ACHOQY_001204"
     CDS             28861..30069
                     /gene="ybjJ"
                     /locus_tag="ACHOQY_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677345.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            30069..30884
                     /gene="ybjI"
                     /locus_tag="ACHOQY_001205"
     CDS             30069..30884
                     /gene="ybjI"
                     /locus_tag="ACHOQY_001205"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9677346.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            30970..31254
                     /gene="ybjH"
                     /locus_tag="ACHOQY_001206"
     CDS             30970..31254
                     /gene="ybjH"
                     /locus_tag="ACHOQY_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9677347.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(31295..32527)
                     /gene="mdfA"
                     /locus_tag="ACHOQY_001207"
     CDS             complement(31295..32527)
                     /gene="mdfA"
                     /locus_tag="ACHOQY_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP9677348.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            32812..33408
                     /gene="ybjG"
                     /locus_tag="ACHOQY_001208"
     CDS             32812..33408
                     /gene="ybjG"
                     /locus_tag="ACHOQY_001208"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9677349.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            33466..34224
                     /gene="deoR"
                     /locus_tag="ACHOQY_001209"
     CDS             33466..34224
                     /gene="deoR"
                     /locus_tag="ACHOQY_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9677350.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(34271..35473)
                     /gene="dacC"
                     /locus_tag="ACHOQY_001210"
     CDS             complement(34271..35473)
                     /gene="dacC"
                     /locus_tag="ACHOQY_001210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9677351.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            35720..36346
                     /gene="gstB"
                     /locus_tag="ACHOQY_001211"
     CDS             35720..36346
                     /gene="gstB"
                     /locus_tag="ACHOQY_001211"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9677352.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(36343..37458)
                     /gene="yliI"
                     /locus_tag="ACHOQY_001212"
     CDS             complement(36343..37458)
                     /gene="yliI"
                     /locus_tag="ACHOQY_001212"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9677353.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(37569..37952)
                     /gene="bssR"
                     /locus_tag="ACHOQY_001213"
     CDS             complement(37569..37952)
                     /gene="bssR"
                     /locus_tag="ACHOQY_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9677354.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            38165..39490
                     /gene="rimO"
                     /locus_tag="ACHOQY_001214"
     CDS             38165..39490
                     /gene="rimO"
                     /locus_tag="ACHOQY_001214"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9677355.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(39537..>40731)
                     /locus_tag="ACHOQY_001215"
     CDS             complement(39537..>40731)
                     /locus_tag="ACHOQY_001215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9677356.1"
                     /translation="AFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPS
                     YLYDVGPYLISSDECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPV
                     THNRSLIFLLGLERFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAM
                     EHFAYLPTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKE
                     AIINNHFERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGG
                     YLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTG
                     GTLEELLSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRA
                     QISWQ"
BASE COUNT         9832 a        10617 c        10302 g         9980 t
ORIGIN      
        1 actatatggg gcaacccgct gttgccctaa atcaaattta ctggatttat ttatctggtg
       61 aatgactcgc cttcgttttt aaaatagccg cgcattattt ccgtcgtcac ttttttacga
      121 cgaccctata aaacgaccat atttttcaca gggtcaattt ttaattgagg tggatatgtt
      181 cagaaaatta gcagctgaat gttttggtac tttctggctt gtttttggtg gctgtggtag
      241 tgctgtactg gccgcaggct tcccggaatt aggcattggt tttgccggcg tggcgttggc
      301 gttcggtctg accgttctga cgatggcctt tgctgttggt catatttctg gtggtcattt
      361 taacccggcg gtcactattg gtttatgggc tggcggacgt tttccggcaa aagaagtcgt
      421 tggctacgta attgcccagg ttgtcggcgg tattgttgca gcggcgctgc tgtatttaat
      481 tgccagtggt aaaacgggtt ttgacgcggc agccagcggt tttgcttcta acggttatgg
      541 cgagcattca ccaggcggtt attccatgct ttccgcgctg gtagttgaac tggtattgag
      601 tgcaggtttc ctgttggtga tccacggcgc aaccgacaaa ttcgcgccgg caggttttgc
      661 gccgatcgct attggtctgg ccttaaccct gattcactta attagtattc cggtgactaa
      721 cacttctgtt aacccggcgc gcagcaccgc ggttgctatc ttccagggcg gctgggcatt
      781 agaacaactg tggttcttct gggtggtgcc aattgtcggc ggcattatcg gtggtctgat
      841 ttaccggacc ctgctggaaa agcgtgatta attaacgcag aaggcctggc gtaatgtcag
      901 gccttttttt aagatgttgt tatatgttga ttcatcatct tgttgcgctc ctttcatgag
      961 ctaagccaca tattgccact ggcgcaagga gcgcgcaggg ggcggccaat cgccgccgcc
     1021 ccatgcaccc ccgggctctg gcgaacaaaa tcgccgctgc gcggtgccct cgacttatcc
     1081 cttacggcta ccgggtcggg cgcgaggtaa catccctgta aaacgcgccc tcagcccaca
     1141 tccatgtggg ctgccccggc cttcagggaa cgcctcggcg attttgacgc caccaacatc
     1201 ggtgcagttt gtaattaaag cgcaagaata tctttaaatg aaaacgaata ctctactgct
     1261 ccttatgcat aacatttcct tttaattgca actttactcg tccttccgct ttcagtagtg
     1321 tgtcatcgcg catttcgact tcatctttgc aaggactggg ttttcatgtt ttctgggctg
     1381 ttaatcattc tggttcccct gattgtgggt tacctcattc cgcttcgcca acaagctgcg
     1441 ttaaaagtta ttaatcagct attaagctgg atggtttacc ttattctctt ttttatgggt
     1501 ataagtctgg cgttcctcga taacctcgcc agtaacctgt tggcgattct gcattattct
     1561 gccgtcagta ttaccgttat tttactgtgt aatattgccg ccctgatgtg gctggagcga
     1621 ggcctgccgt ggcgcaacca ccatcagcaa gaaaaactcc cgtcgcgtat tgcgatggcg
     1681 ctggagtcgc taaaactgtg cggcgtagta gtgattggtt ttgccattgg tctgagtgga
     1741 ctggctttct tacaacacgc gaccgaagcc agtgaataca cgttaatttt gctacttttc
     1801 ctcgttggta ttcagttgcg caataatggc atgaccttaa agcagattgt ccttaatcgc
     1861 cggggaatga ttgtcgccgt ggtggtggtt gtcagttcat taattggtgg tttaattaac
     1921 gcctttattc ttgatctccc catcaatacc tcgctggcaa tggcctccgg tttcggctgg
     1981 tattctcttt ccggtatttt attgaccgaa tcttttggtc cggtaatcgg gagcgcggcg
     2041 ttttttaatg atctggcccg tgaactgatt gctattatgt tgatccctgg gctgattcgc
     2101 cgcagccgct ctactgcact gggcttatgc ggtgccacat caatggattt caccctgccc
     2161 gttcttcaac gtactggcgg gctggatatg gtcccggcgg caattgttca cggttttatt
     2221 cttagcctgt tagtgccgat cctcatcgcc tttttctctg cgtaatacct ctctggcggt
     2281 agattcctgc cgccaaaatt gcgctaaatc aatctccctt aaagttgcat gaaaaatccc
     2341 ttttatcccc gcgttaagcg tcttaacctt aaacatgtat attaaatata actttaaaag
     2401 gtgtgatcat gttttgtgtg caatgtgaac aaactatccg tactccggca ggaaacggct
     2461 gctcatacgc gcaggggatg tgtggtaaaa cggcggaaac ttctgacctt caggatttac
     2521 tcatcgcggc gctgcaaggg ctttctgcct gggcggtaaa agcgcgtgaa tacggcatca
     2581 tcaaccacga tgttgacagc ttcgcgccac gtgccttttt ctcaactctg accaacgtta
     2641 acttcgattc tccgcgtatt gtcggctacg ctcgtgaagc gattgccctg cgcgaggcgc
     2701 tgaaagcgca atgcctggct gtagatgcca acgcgcgcgt cgataacccg atggctgacc
     2761 tgcaactggt gagcgacgat ctcggcgagc tgcaacgtca ggctgcagaa tttaccccta
     2821 acaaagataa agcggcgatt ggcgaaaaca ttctcggcct gcgtctgctg tgcctgtatg
     2881 gcctgaaagg tgcggcggcc tatatggaac acgcgcatgt tctcggtcaa tacgacaacg
     2941 atatttatgc ccagtatcat aaaatcatgg cgtggctggg gacctggcct gccgatatga
     3001 acgcgcttct tgagtgttca atggaaatcg gccagatgaa cttcaaagtg atgagcattc
     3061 tggatgcagg cgaaaccggt aaatacggtc acccgacgcc aacccaggtc aacgtcaaag
     3121 cgacggcggg taaatgcatt ctgatttccg gtcacgatct caaagatctc tacaacctgc
     3181 tggaacagac cgaaggcacg ggcgttaatg tctacaccca cggcgaaatg ctgcctgcgc
     3241 atggctaccc ggagctgcgt aaattcaagc atctggtcgg taactacggc agtggctggc
     3301 agaatcagca agtggagttc gctcgtttcc ctggccccat cgtgatgacc tcgaactgca
     3361 tcatcgaccc aaccgtaggc gcttatgacg atcgtatctg gacccgcagc attgttggct
     3421 ggcctggcgt gcgtcatctg gacggtgatg atttctctgc ggttatcacc caggcgcaac
     3481 agatggcagg cttcccgtac agcgaaattc cgcaccttat caccgtgggt tttggtcgcc
     3541 agacgctgct tggcgctgct gatacgctga ttgatctggt gagccgtgaa aaactgcgtc
     3601 atatcttcct gcttggtggc tgtgacggcg cacgcggcga gcgccactac ttcaccgatt
     3661 tcgccaccag cgtgccggat gactgcctga tcctgaccct cgcctgtggt aaatatcgct
     3721 ttaacaaact tgagtttggc gatatcgaag gtctgccgcg tctggtagat gcaggtcaat
     3781 gtaacgatgc ttactcagcg attattctgg ctgtcactct ggcagagaaa ctgggctgcg
     3841 gtgtcaacga tctgccgctg tcgctggtgc tctcctggtt tgaacagaaa gcgatcgtca
     3901 ttctgctgac gctgctttct ctgggcgtga aaaatatcgt caccggcccg actgcacctg
     3961 gtttcctgac accagacctg ctggcggtgc tgaacgagaa atttggcctg cgttctatca
     4021 ccactgttga agaagacatg aagcaactgt tgagcgcgta aggagtttgt cgatgacgat
     4081 gccaacgaat caatgcccgt ggcggatgca ggttcatcac attacgcaag aaacgccgga
     4141 tgtgtggacg atttccctga tttgccacga ttactaccca tatcgcgccg ggcaatatgc
     4201 actggtcagc gtgcgaaact cagcggaaac gctgcgtgct tacaccattt cctccacgcc
     4261 aggcgtgagt gaatatatca ccctgaccgt gcggcggatt gatgacggtg tcggctccca
     4321 gtggctgacg cgcgatgtaa aacgcggtga ttatctctgg ctttcggacg cgatggggga
     4381 atttacctgc gacgataaag cagaagataa attcctgttg ctggcggcag gctgcggcgt
     4441 cacgccgatt atgtcgatgc gtcgctggct tgcgaagaac cgtccacagg ccgatgtgcg
     4501 ggtgatctac aacgtgcgta cgccgcagga tgttattttc gccgatgagt ggcgtaacta
     4561 tccggtaacg ctggtggcag aaaataacgt taccgaaggc tttatcgctg gtcgtctcac
     4621 tcgcgaactg ctggcaggtg tacctgactt agcttcacgt accgtgatga cctgcggccc
     4681 ggctccgtat atggattggg tagagcagga agtgaaagcg ctcggcgtga cgcgtttctt
     4741 taaagagaaa ttcttcaccc cagtagcaga agcggcgacc agcggtctga aattcaccaa
     4801 actgcaaccg gcacgagaat tttacgcccc ggttggcacc acgctactgg aggcgctgga
     4861 aagcaataac gttccggttg tcgctgcctg ccgtgcgggt gtttgcggct gctgtaagac
     4921 aaaagtggtt tccggtgaat atacggtgag cagtacaatg acgctgaccg acgccgaaat
     4981 cgctgaaggt tacgtactgg cctgctcctg ccatccgcag ggggatttgg ttctcgcata
     5041 atcgccttat gcccgatgat attcctttca tcgggctatt taaccgttag tgcctccttt
     5101 ctctcccatc ccttccccct ccgtcagatg aactaaactt gttaccgtta tcacattcag
     5161 gagatggaga accatgaaac aaacggttgc agcttatatc gccaaaacac tcgaatcggc
     5221 aggggtgaaa cgcatctggg gagtcacagg cgactctctg aacggtctta gtgacagtct
     5281 taatcgcatg ggcaccatcg agtggatgtc cacccgccac gaagaagtgg cggcctttgc
     5341 cgctggcgct gaagcacaac ttagcggaga actggcggtc tgcgccggat cgtgcggccc
     5401 cggcaacctg cacttaatca acggcctgtt cgattgccac cgcaatcacg ttccggtact
     5461 ggcgattgcc gctcatattc cctccagcga aattggcagc ggctatttcc aggaaaccca
     5521 cccacaagag ctattccgcg aatgtagtca ctattgcgag ctggtttcca gcccggagca
     5581 gatcccacaa gtactggcga ttgccatgcg caaagcggtg cttaaccgtg gcgtttcggt
     5641 tgtcgtgtta ccaggcgacg tggcgttaaa acctgcgcca gaaggggcaa ccacgcactg
     5701 gtatcatgcg ccacaaccag tcgtgacgcc ggaagaagaa gagttacgca aactggcgca
     5761 actgctgcgt tattccagca atatcgccct gatgtgtggc agcggctgcg cgggggcgca
     5821 taaagagtta gttgagtttg ccgggaaaat taaagcgcct attgttcatg ccctgcgcgg
     5881 taaagaacat gtcgaatacg ataatccgta tgatgttgga atgaccgggt taatcggctt
     5941 ctcgtcaggt ttccatacca tgatgaacgc cgacacgtta gtgctactcg gcacgcaatt
     6001 tccctaccgc gccttctacc cgaccgatgc caaaatcatt cagattgata tcaacccagc
     6061 cagcatcggc gctcacagca aggtggatat ggcactggtc ggcgatatca agtcgactct
     6121 gcgtgcattg cttccattgg tggaagaaaa agccgatcgc aagtttctgg ataaagcgct
     6181 ggaagattac cgcgacgccc gcaaagggct ggacgattta gctaaaccga gcgagaaagc
     6241 cattcacccg caatatctgg cgcagcaaat tagtcatttt gccgccgatg acgctatttt
     6301 cacctgtgac gttggtacgc caacggtgtg ggcggcacgt tatctaaaaa tgaacggcaa
     6361 gcgtcgcctg ttaggttcgt ttaaccacgg ttcgatggct aacgccatgc cgcaggcgct
     6421 gggtgcgcag gcgacagagc cagaacgtca ggtggtcgcc atgtgcggcg atggcggttt
     6481 tagcatgttg atgggcgatt tcctctcagt agtgcagatg aaactgccag tgaaaattgt
     6541 cgtctttaac aacagcgtgc tgggctttgt ggcgatggag atgaaagctg gtggctattt
     6601 gactgacggc accgaactac acgacacaaa ctttgcccgc attgccgaag cgtgcggcat
     6661 tacgggtatc cgtgtagaaa aagcgtctga agttgatgaa gccctgcaac gcgccttctc
     6721 tatcgacggt ccggtgttgg tggatgtggt ggtcgccaaa gaagagttag ccattccacc
     6781 gcagatcaaa ctcgaacagg ccaaaggttt tagcctgtat atgttgcgcg caattatcag
     6841 cggacgcggc gatgaagtga tcgaactggc gaaaacgaac tggctaaggt aaaaagggtg
     6901 gcatttcccg tcataataag gacatgccat gattgattta cgcagtgata ccgttacccg
     6961 accgagccgc gccatgctcg aagcgatgat ggccgccccg gttggggacg acgtttacgg
     7021 agacgaccct accgttaatg ctctgcagga ctatgcagca gagctttccg gtaaagaagc
     7081 cgccattttt ctgcctaccg gcactcaggc caacctggtc gctctgctca gtcactgcga
     7141 acgtggcgaa gagtatattg tcggtcaggc cgcgcataac tatctgtttg aagccggtgg
     7201 cgcagcggtg ctgggcagta ttcagccgca acccatcgac gcggctgccg acggcacgct
     7261 accgctggat aaagtggcga tgaaaatcaa acccgacgat atccatttcg cccgcaccaa
     7321 attactcagt ctggaaaaca cccacaacgg caaagtgctg ccgcgtgaat acctgaaaga
     7381 agcatgggaa tttacccgcg agcgcaatct ggcgctgcat gttgacggtg cgcgcatctt
     7441 taatgccgtg gtggcttacg gctgcgaact gaaagagatc acgcaatatt gtgattcgtt
     7501 caccatttgc ctgtcgaaag gtcttgggac gccagtcggt tcattactcg tcggtaatcg
     7561 tgattacatt aaacgtgcca tccgctggcg gaaaatggcc ggtggcggga tgcgtcagtc
     7621 cgggattctg gctgccgccg ggatgtatgc cctgaaaaat aacgtcgcac ggttgcagga
     7681 agatcacgac aacgccgcct ggatggcgga gcaactgcgt gaagcaggcg cggatgtgat
     7741 gcgtcaggac actaatatgc tgtttgttcg cgtcggggaa gaaaatgctg ccgcgttagg
     7801 cgaatacatg aaagcgagaa acgtgctgat taacgcctcg ccgattgtcc gcctggtgac
     7861 gcatcttgac gtctcgcgcg aacaactggc ggaagtcgcc gcccactggc gcgcattcct
     7921 ggcgcgttaa ggagagagac gtgccgcaac gcattttagt tctcggtgcc agtggctaca
     7981 ttggtcagca tctggtgcgc acactcagcc agcaagggca tcagatcctg gcggcggcac
     8041 gtcatgtcga caggcttgca aagctgcaac tggcaaacgt cagttgccat aaagtcgatc
     8101 tcagctggcc ggataacctt ccggccctgt tgcaggatat cgataccgtc tattttctgg
     8161 tgcacagcat gggcgaaggc ggcgatttta tcgctcagga gcgccaggtg gctctcaacg
     8221 tccgcgatgc gctacgtgaa gtaccagtta agcaattaat ctttctcagt tcgttgcagg
     8281 ccccgccaca tgagcagtcg gatcatctgc gtgctcgtca ggctacggcg gacattcttc
     8341 gtgaagcgaa tgtacctgtg accgaacttc gggccggaat tatcgttggc gcaggttcag
     8401 cggcgttcga agtcatgcgc gatatggtct acaacctgcc agtgttaacg ccgccacgct
     8461 gggtacgttc acgcaccacg cccatcgcgc tggaaaactt gctgcactat ctggtggcgt
     8521 tgttagatca tccggccagc gaacaccgca tcttcgaagc cgccggacca gaggtgctca
     8581 gttatcagca acagtttgaa cattttatgg cggtgagcgg taagcgccgc tggttgatcc
     8641 ccatccccct ccccacccgc tggatttcgg tgtggtttct caatgtgatt acttccgtac
     8701 cgcccaccac cgccagggcg ttgattcagg ggctgaaaca cgatctgctg gcggatgata
     8761 ccgcgctacg tgcactcatc ccacaacggc tgatcgcttt cgatgacgcg gtacgtagca
     8821 cgttgaaaga ggaggaaaaa ctggtcaact ccagcgactg gggctacgac gctcaggcct
     8881 ttgcccgctg gcgaccagag tacggttatt ttgccaaaca ggcggggttt accgttaaaa
     8941 cgtccgccag ccttgctgct ttatggcagg tagtgaacca aatcggcggt aaagagcgtt
     9001 atttctttgg caatattttg tggcagacac gggcgttgat ggaccgcgcg atcggtcata
     9061 agctggcgaa aggccgcccg gagcgcgaat atttacagac tggcgatgcg gtggatagct
     9121 ggaaagtgat tgtcgttgaa ccggaaaaac aacttacgtt gttatttggc atgaaagcgc
     9181 cggggctggg acgactgtgt tttagcctag aagataaagg cgactatagt actatcgatg
     9241 tccgcgcttt ctggcatccg cacggtatgc cggggctgtt ttactggtta ttgatgatcc
     9301 ccgcgcatct gtttattttt cgcggaatgg caaaacaaat cgccagactg gcagaacaaa
     9361 gcacagatta atcgacaaaa cctgcgttat tctttcatgt ttcccattgc atcatggggg
     9421 aaatcacgga agaatgcgca cgttattttc tcaacaacag tggattgata tgaaggtact
     9481 ggttaccggc gccaccagcg gcttaggtcg aaacgcggta gagtttttat gccagaaagg
     9541 catcagcgtg cgagcgaccg gtcgcaacga ggcaatgggc aaattgctgg aaaaaatggg
     9601 cgcagagttt gttccggcgg atctgaccga gctggtttca tcacaagcta aagtgatgct
     9661 cgcgggcatt gatacgctgt ggcactgctc cagctttacc tcaccctggg ggacacaaca
     9721 ggctttcgat ctggctaacg ttcgcgccac tcgccgcctg ggtgaatgga ctgtcgcctg
     9781 gggtgtgcgt aactttattc atatctcttc cccctccctg tacttcgatt atcaccacca
     9841 tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc aacgagtttg cccgcagcaa
     9901 agcagccagc gaagaagtga tcaatatgct ttcgcaggcg aatccacaaa cgcgctttac
     9961 tattctgcgc ccacaaagtc tgttcggacc gcacgataaa gtctttattc cccgtctggc
    10021 gcatatgatg caccactacg gcagtattct gttaccgcat ggcggcagtg cgctggtcga
    10081 tatgacctac tatgaaaatg ccgtgcacgc aatgtggctg gcaagccagg aagcctgcga
    10141 taagctacct tccgggcgtg tgtacaacat caccaacggc gagcatcgca cactgcgcag
    10201 catcgtgcag aagctgatcg acgagttgaa tattgactgt cgtattcgtt ccgtccccta
    10261 cccgatgctg gatatgatcg cccgcagcat ggagcgttta ggccgcaagt cagcaaaaga
    10321 gccgccgctg acccactacg gcgtctccaa gttgaatttt gactttacgc tggatattac
    10381 gcgtgcgcag gaagagttag gttatcagcc ggtcatcacc ctggatgaag gtatcgagaa
    10441 aactgccgcc tggctacgcg accacggaaa actgccgcgc taatcctgcc cgtatttctc
    10501 cagcaatgct tcggcaatcg cctgagtttc agcatccgct tcgccgttat ataacgtcgg
    10561 gcggaaatgc atctggaatg ccataatcac gcgccgctgc tcgcgcggtg tcatatcagg
    10621 tttaacgtca taaccgtagc gcgccaaaag ctccagcaat gacgcggtat ctaccggtgt
    10681 gtgcggcgcg cgcccggcaa ggtaaaagtt aacccgctgc gcatccggcc aggcaccaat
    10741 cccctgctgc gccagttgct gccagggaaa taatggcccc ggatcgtctt tgcgctgcgg
    10801 tgcgatatcc gcatgtgcca ctacgttttc cggcttgatg tgataacggg caataatatc
    10861 tttcgccagt ggaataagcg cctgaatctg tgccggttca aacggggcaa aatatttcac
    10921 tccggccgat ttttgccagc cacggttttc cagctcaatg ccaatagagg tgtcgttaag
    10981 gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag gccagttctt gttccggcac
    11041 cagttgccag atgcgcggtt taccgttgta tcgcggtggt accgcaggga ccagataatg
    11101 cgagctgacc tgcttatcgg tcagtgtcgc cagcgagcta tcaaaatcat ctgcggtgta
    11161 gtggatcacc agcactttaa tgcgcggata cgccgcctgc gcctggcgtc gggtatcaag
    11221 ctgatatccc tctttctcga caatgccttt ttcgcctgca caccctgcca gtaacagagc
    11281 ggcagcgatc agccagaaga ctcttctcat cgacgcgttt tcaccgccgt accgctgacg
    11341 ctaaccatca gcatactgcc gttttgcccg accgtttcgt agtcgatatc aataccgacg
    11401 acggcatcgg cccccagcgc ccgcgcctgg gagcctaatt cctcaaaggc gatctcccgt
    11461 gctttacgca gttctttttc atacgcaccg gaacgtccgc caacgatatc gcggataccg
    11521 gcaaagaaat cacggaaaat attggcacct aaaatcgctt cgccggtcac cacaccgcaa
    11581 tattcaacga tgatctggcc ttccagagtt ggggttgtag aaaattgcat gatcgatctc
    11641 cttgtttgtt aagccttcga tctctaaagc attatcagac tgatacgcta ttattgaaag
    11701 gatatcatta ttatcgtaga acgctttcag agcgatcgct tataaggaaa tcattatgcg
    11761 ctacagcaaa ttgacaatgc ttatcccctg cgcactgctc ctcagtgcct gcaccacagt
    11821 cactccagct tataaagata acggcacacg cagtggtcct tgcgttgaag gaggcccgga
    11881 taacgtggcc cagcaatttt atgactaccg cattttgcac cgcagcaacg acatcaccgc
    11941 cttgcgccct tatctgagcg acaaactggc gacactgctt agcgatgcct cccgggataa
    12001 taaccatcgg gaactactga ccaacgatcc attctccagt cgcacaacct tgccagagag
    12061 cgcccgtgtt gccagtgcct caacaatccc gaatcgtgat gcgcgaaata ttcctctgcg
    12121 tgtggatcta aaacagggtg atcagagctg gcaggatgaa gtgttaatga ttcaggaagg
    12181 ccagtgttgg gtgatcgacg atgtacgcta tttaggcggc agcgttcatg ccacggcggg
    12241 tacgctccgt cagtccatcg agaatcgcta acgccttgat caaccaataa accccgtaaa
    12301 ttgtccggca tttcgtgagg aatgccgaca tttatgctcg ccgaccaccg cccccgttat
    12361 tttgtgctat gtttattgaa taatgcgctt tgcttttaac ttttaaagca gaaatattgc
    12421 ataattattc tgtcaaaggt actatcttcg acctcaatag ctatcagact gccagtatac
    12481 gagtgtcaat gagtattcaa ttaaacggca ttaattgctt ctacggcgcg catcaggcgc
    12541 tgttcgatat cacgctggat tgcccacagg gcgaaacgct ggtgttactt ggccccagcg
    12601 gcgcgggtaa aagctcgctg ctgcgtgtac tcaatctgct tgagatgccg cgctccggta
    12661 cgctcaacat tgcaggcaac catttcgatt tcaccaaaac accctctgac aaagcgattc
    12721 gcgatttgcg tcgtaacgtt ggcatggtgt ttcagcaata caacctgtgg ccgcatctga
    12781 ccgtgcagca aaacctgatt gaagcgccct gccgtgtact ggggttgagt aaagatcagg
    12841 cgctggcccg tgcagaaaaa ctgctggaac gtctgcgtct caaaccttat agcgatcgtt
    12901 acccgctgca tctttctggt ggtcagcagc agcgtgttgc tattgcccgt gcgttgatga
    12961 tggaaccgca ggtactgctg ttcgatgaac cgaccgccgc actggacccg gaaattacgg
    13021 cacaaatcgt cagcatcatt cgtgagctgg cagaaacgaa tattacccag gtgatcgtca
    13081 cccacgaagt tgaagtggcg cgtaaaaccg ccagccgagt ggtgtatatg gaaaatggtc
    13141 atatcgtaga acaaggcgac gcgagctgct ttaccgagcc gcaaaccgaa gcatttaaaa
    13201 actatctctc tcactaagat tcgggataac aacaatgaaa aaagttctga ttgccgcgtt
    13261 aattgcaggt tttagtcttt ccgccacagc tgccgaaacc attcgttttg ctaccgaagc
    13321 atcctatcct ccgtttgaat cgattgatgc aaacaaccag atcgttggtt ttgacgtcga
    13381 cctggcacaa gcgctgtgta aagagattga tgcaacctgc actttctcta accaggcgtt
    13441 tgacagcctg atcccaagcc tgaaattccg tcgcgtagaa gccgtgatgg cgggcatgga
    13501 tatcactccg gagcgtgaaa agcaggtgct gtttaccacc ccgtactatg acaactctgc
    13561 cctgtttgtg ggtcagcaag gcaaatacac cagtgttgat cagctgaaag gcaaaaaagt
    13621 cggcgtacag aacgggacga cacaccagaa attcattatg gataagcacc cggaaatcac
    13681 taccgttccg tatgacagct accagaacgc aaaactggat ctgcaaaacg ggcgtatcga
    13741 cggcgtcttc ggtgacaccg cagtggtcac tgagtggctg aaagataacc cgaaactggc
    13801 ggcggtgggc gacaaagtga ccgataaaga ttacttcggc actggcctcg gcatcgcggt
    13861 acgtcagggc aacactgagc tgcagcagaa actcaacact gcgctggaaa aagtgaagaa
    13921 agatggcact tacgaaacca tctacaacaa atggttccag aagtaattcc tgatgaatga
    13981 attttttcct ttagcaagcg ccgccgggat gaccgtcggc cttgccgttt gtgcattgat
    14041 tgtcgggctg gcgctggcga tgttctttgc ggtatgggag tcggcaaaat ggcgtcctgt
    14101 cgcgtgggca ggttcagcgc tggtaaccat tctgcgtggc ctgccagaaa ttctggtggt
    14161 gctgtttatc tattttggct cctcgcagct gctgctgacg ctttcggatg gcttcactat
    14221 caatcttggg ttcgtgcaga tcccagtgca gatggacatt gagaacttcg acgttagccc
    14281 gttcctttgt ggtgtcatcg ctctgtcact gctgtatgcc gcctatgcct cgcaaacgct
    14341 tcggggcgcg ttgaaagcgg tgccggtggg tcagtgggag tctggtcagg cgctggggct
    14401 gtcgaaatcg gctatctttt tccgtctggt gatgccgcag atgtggcgtc atgcgctgcc
    14461 tggcctcggt aaccagtggc tggtgctgct gaaagatacc gcgctggtca gtttgattag
    14521 tgtgaatgat ttaatgctgc aaacaaaaag catcgctact cgtacccagg aaccatttac
    14581 ctggtacatt gtggcggcgg cgatttacct ggtgatcacc ctgctcagtc agtacattct
    14641 caaacgcatt gacctgcgcg cgacacgttt tgagcggagg cccagctaat gtttgagtat
    14701 ttacccgaac tgatgaaagg gctacacacc agcctgacgc taaccgttgc ctcgctgatt
    14761 gtggcactga ttctggcatt gatttttacc atcatcctga cgctgaaaac gccggtgctg
    14821 gtgtggctgg tgcggggtta tatcacgctg tttaccggta cgccgctgct ggtgcagatc
    14881 ttcctgattt attacgggcc gggccagttt ccgactttgc aggagtatcc ggcactgtgg
    14941 catttgttgt cagaaccgtg gttatgtgcg ctgattgcgt tatcgctgaa cagtgcggcg
    15001 tataccacgc agctgtttta cggtgcaatt cgtgcgatcc cagaaggtca gtggcagtcc
    15061 tgtagcgccc tgggaatgag caaaaaagat acgctggcga tcctgctgcc gtatgccttt
    15121 aaacgctcgc tctcttctta ttccaacgaa gtggtgctgg tgttcaaaag tacctctctg
    15181 gcttacacca ttacgctgat ggaagtgatg ggatacagcc agttgttgta cggacgcacc
    15241 tacgatgtaa tggtgttcgg tgcggcaggg attatttacc tggtcgtcaa cggcctgctg
    15301 acgctgatga tgcgtctgat cgagcgcaaa gcactggcat tcgaacggcg aaattaatgt
    15361 agctgcgtac attcgccggg ggggtttgta ggtctgataa gacgcgccag cgtcgcatca
    15421 gacatcagca cggtgcctgt gccggatgcg gcgtggacgc cttatccggc ctacatagtg
    15481 cataaattct tataatgaag acggacaact cactaaggta tccgtctttt tttatttcat
    15541 ttaaattatt taatcatatt tattgcatat aaattcactt gatggcattg ttatcccatg
    15601 ccgcagacac ggccaaaaat cataagattg acagacggga gttccatcat gaaaaagtta
    15661 gttcttgccg ctttacttgc ttcctttact ttcggtgctt ctgccgcaga gaaaatcaat
    15721 tttggcgttt cagccaccta tccacccttt gaatctatag gtgctaataa tgagatggtc
    15781 ggctttgata tcgatctggc aaaagccttg tgcaaacaaa tgcaggcaga atgtactttt
    15841 actaatcacg cgttcgacag cctgatcccg tcactgaaat tcagaaaata tgacgccgta
    15901 atctccggta tggatatcac gcccgagcgt agcaagcagg tatcgtttac cacgccctac
    15961 tatgaaaact cagccgtcgt gattgccaaa aaagatacct acaaaacgtt tgccgatctg
    16021 aaaggcaaac gtattgggat ggaaaacggc accacgcacc agaaatatat tcaggatcag
    16081 caccccgaag tgaaaactgt ctcctatgac agttatcaga atgcctttat cgatctgaaa
    16141 aatggtcgta ttgatggggt atttggtgac acagcggtgg taaacgaatg gctgaaaacc
    16201 aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc cgcaatactt cggcaccggt
    16261 ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg aaaaactgaa taacgcgctg
    16321 gcagcaatta aagctgacgg tacatatcag aaaatcaatg accagtggtt tccgcagtaa
    16381 ttgagactga tgacaaaagt aagattgcct gatgcgctgc gcttatccgg cccacatcaa
    16441 ctctgcaatg tattgattta gcatgatttt gtaggccgga taaggcgtac acgccgcatc
    16501 cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg cacctgcggc ttttttattg
    16561 cttcaccagc agcgtcagca cttcatagtg cgcggtgtgc gggaacatat caaaaagctg
    16621 tacccgttcg atgcggtaac ctggcagttc gcggatatct ttcgccatgg tttgggcgtt
    16681 acagctggag tagataataa aacgcggtgc cattgttgag agataatcac atagcggttt
    16741 accaatgccg cggcgcggcg gattaaccag caccagctcc ggcacctccc cctgagcggt
    16801 ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc
    16861 tgactgcttt gcacaggcaa tggcctctga tgcaatttcg atcccggtta actgcatgtc
    16921 aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag aacagatccc acatatgttt
    16981 aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac agctggctgg cgaccgccgg
    17041 attagtctgg aagaaacttt gcggacggat ccacagcggt acgtcattaa aacgctctgc
    17101 cagcgcctgt tgttcagtaa ggtagatctc cgtctcccct tccataatcg ccatatgtac
    17161 cggctgaata ttgacggtaa taactttcag ttgcggtagt tgttcctgta accacggcag
    17221 cgccttacgc agttgtgcca gtttggtttc agaacgcagt acaaagcgca gcatcatgcc
    17281 gccatcgctc tggctttcag tcagcagaat gtatttcagt tcgccacgct tacgcgccac
    17341 gttgtagggt gtcagccccg cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc
    17401 aaatgaggca ggataaagcg ggcagtcaca aaggtcttct ggtgtgccgt cgcgatgcag
    17461 cataccgagc agtggttttt caacgctacc actcaccacc attttggctt tattacgaaa
    17521 cccttgttcc gggcctgaca ccggcgcgca ccattcctca accggaaaat cggcgagcag
    17581 atttttaaga tcggcggttt tagcggagag ttgctctgga atcggctgcg ttatccactg
    17641 acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc atacattgac cttcacatca
    17701 tcaggggcga cgattataca cattattgca actggaagaa ccgccgactg gtcgagggaa
    17761 cgaacagcag catcaggatg agcatatccg gcagcttctg cagcatcagg ctatggaaga
    17821 tttcacgttt tgattcaccg ggaatgctga acagctccgg ataaccgtac cccagcgaag
    17881 ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag atatagccag cgcgcccagc
    17941 gacgcccttt caccagtgaa aacgcacacc agatctcaat gaaaaccagc accagactgc
    18001 ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg atgaatgaac tcgccaatac
    18061 cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag gccacggatc ataataatgg
    18121 cgagcgccgc cacctgcacc agcgcaggca cattcaggcg agcatgagat gaagatgttt
    18181 tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat gccgcggcat gcgcggcatt
    18241 atgtagccag gttggcaaat tttagtgtct tcagccatgt cttgcacgct ggatatcgcg
    18301 tatccgctgt ttttctgcgc gagccatcag ataccaggcg ataaatccga caattccgac
    18361 cgcaccgaga attaacgttg caagggcgtt gatttccgga ttcaccccca tccgcacgct
    18421 ggaaaagacc agcatcggta acgtggtggc tcccggcccg gaaacaaagc tggcgatcac
    18481 cagatcatca agcgacaaag taaaagccag taaccagcca gaaatgatcg cgggcatgat
    18541 catcggtagc gtaatgacga aaaacacttt cagcggcgtc gcaccgagat ccatcgctgc
    18601 ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa ataacgaccg ccacataagc
    18661 cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata ccgcggtccg caggccagcc
    18721 aatggcatga gcaagcgcga cgaataataa caacagcgac aagcccgtga tgacatctgg
    18781 catcaccagc ggcgcggtga tcataaaggc aaagccattt gatccgcgaa acctgccaaa
    18841 gcgcaccagc accaccgccg caatagtccc gaggatcgcc gccgccgttg ccgcacaggc
    18901 agcaattgtc aggcttaaac caaccgcact catcatcgcg tcatcgcgca ataactcacc
    18961 ataccagcgc gttgaccagc cggcccacac cgtcaccagc ttcgagctgt taaacgaata
    19021 gataaccagc atcagcattg gcgcgtagag aaaggtaaag cccagcagca aaatcacaat
    19081 ccgccagggc gaacgaacta ccggtaaatt attcatccgt gttctcccac gcttttttgc
    19141 tggtgtttgt ggaaccacat tatcggcaca attagcagca gcaacatgat gatcgctacc
    19201 gccgaggcca ccggccagtc gcggttattg aaaaactctt gccatagcac gcgcccgatc
    19261 atgatgctgt ccgggccacc gagcagttcc gggatcacaa actcgcccac agccgggata
    19321 aacaccagca tcgatccggc aataatccca cctttagtca gcggcacgat cacagtaaag
    19381 aacgttttca gcggtcgtgc accgagatcc agcgctgctt ccaccagcga ataatcaata
    19441 cgaatcaacg cggtataaat cggcagtacc ataaacggca cgtaggcgta aacaatgcca
    19501 atataaactg ccagattggt atgaagaatg gtcagcggtt gatcgataac ccccagccac
    19561 agcagaaaat tattcagcac accgttgttt tttaatattc ccatccaggc ataaacgcgg
    19621 atcagaaacg aggtccacga cggcaggatc accagtagta ataaaatatt acgggtcgaa
    19681 ggcttactgt gcgccaccgc ccacgccagc ggatagccga tcagtaaaca gcaaaatgtc
    19741 gaaatcgcag ccacctgtag cgactggaga taagcatcga aatagagcgg atcgtcggtc
    19801 agttgcagaa aattaccgag attaagagtg atggacagtt gcccgtcagc ccactccatc
    19861 agttcggtat aaggtggaat agcgcgcgcc atctccgcca ggcttatttt aaagacaatc
    19921 agaaatggca gcagaaacag caagatcaac cagatatatg gcaacgcaat gaccagtttg
    19981 cgcccatgct tcatttgcag ctgcgacaac cacagcttaa atccgcccgg cggtttcgac
    20041 tgggcagcag gttcaagtgt attcatcttt gctccttaaa ccgtcagcac cacacagctg
    20101 tccacttccc agcacaaacg cacttcgtcg ccccaggtcg gtaacccttt acgatgacga
    20161 tgggcgtttt gtagctgggc gctaatcatc tgcccacttt tcagacgaac gtgatacacc
    20221 gaaagatcgc cgagataggc aatgtgtatc acctccccca ccgcgaagtt acaaccattg
    20281 gcgggcggct cttcgcaaag catgattttt tccgggcgca gcgctacgtg taccggcacg
    20341 ttatcgacca ctgaagcatc cgcatcgact ttcagtggat gcaccagccc cggcgaatca
    20401 agcaccaggc catcttcctg acgctctttg agtacacctt caaagacatt aaccgaaccg
    20461 ataaattcag cgctgtagcg ggtagttgga tgctcgtaga tctcttccgg ctcgccaatc
    20521 tggacaaatt tcccgcgatt cataatggcg atacgccccg ccatggtcat cgcctcttcc
    20581 tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct ccagaatatc caccacttca
    20641 agctgcatcc ggtcacgcag ctttttatcc agcgcgccca tcggctcatc gagcagtaat
    20701 agtttcgggc gtttggcaag gcttcgggcc agggccacac gctgtcgctg accaccggaa
    20761 agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca ccagcccaag catctcgttg
    20821 acccgggtgg caatttccgc tttcggtagt ttgtcctgtt tcaggccaaa ggcgatgttc
    20881 tgctccacag tcatatgggg aaacagcgcg taagactgaa acatcatatt gatggggcgc
    20941 aggtaaggcg gcacctgtga caaatcgacg ccatcaagca ttatctgtcc ggcagaaggt
    21001 tgttcgaaac ctgccagcat acgcagcagc gtggacttgc cacagccgga tgcgcccagc
    21061 agcgcgaaga tttcaccttt gtagatggtc aggctgacat catccaccgc atgttgacca
    21121 tcgtaggatt tggtgaggtt gcggatttct aatagcggcg tcagcgcctt acgggttttc
    21181 gcctgcgggc gagggatagc gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc
    21241 gcaccatgat ggtgcgaatt gccggcgggt gtggtgcgcc cgcccggcat ctaaggctgc
    21301 ggattatttt ccgctcttca ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg
    21361 gtcctgcact ttcagagtga acagcttcgc acgaacatcc gcaggcggat aaatacccgg
    21421 gttctcacgg acttccgcac tcaccagcgg cgtggctgct ttattggcgt tggcatagaa
    21481 cacattgtcg gaaatatgcg ctactacatc cgggcgcagc aggtaattca ggaactgata
    21541 ggcttcgtct ttgtttttgg catccgcagg catggcgaat acatcaaaga acgccatcgc
    21601 cccttctttt ggaatcgaga acgagacatt cactccattc ttcgcttcct tcgcgcggtt
    21661 tgacgcctgc cagacatcac ctgcccagcc gatagcgacg caaatatcgc cgtttgccag
    21721 gtcgttaatg tattgagatg aatggaaata acgaatgttc gggcgcagct ttaacagcag
    21781 atcggttgcc ggtccggtgt aatcatccgc tttagtgctg ttgggatctt tgccgagata
    21841 attcaacacg gtagcaaaaa cttcttctgg cgcatccagg aaagagacac cgcagctttt
    21901 cagtttttcc agattttcag gtttgaggat caagtcccag ctatcgacgg gcgcgttttc
    21961 gcccagcaca gctttaactt tatcaacgtt atagccaatc ccggtcgtcg cccacatata
    22021 gggcatagca aatttattgt cgggatcgtg tttggcgacc agcttcagca gctccgggtc
    22081 gagattcttc cactccggca atttgctttt gtccagcggc tggaaaactc ccgcagtcaa
    22141 ctggcgttca agaaagctgg cagatggaac caccaagtca aagccggtgc tacccgccat
    22201 taatttgcct tccagtactt cgttagagtc gaaaacatcg tagacgactt taataccggt
    22261 ttctttttca aaattggcca ccgtgtccgg ggcgatataa tcagaccagt tataaacgtg
    22321 gagtgttttt tgttcagccg cgagcgtgcc gacagagacg gccatcagag cacccgcagc
    22381 cagacccgaa agccattttt tatttaaggc ggtcatatct ctttccttct gaaagttcgt
    22441 taacaaacga acaaaagttg tcacactaaa agatatgcaa taaacgtgca tattttttat
    22501 cggcggggtg taagaaaaga gaactctctc ccggccagca ctgctcgcta tataaaagca
    22561 tcgcaagaat aactttagcc agggtttgag actatagctc ggattaaaaa acggtattta
    22621 acaaattttt atgcgtattt gctctatgtg ataaagccaa atcgacacaa gagaagtgaa
    22681 ataatcttta agaaaaggtt aaaaacagca gataattttg cagcgcgcag ccactgcacc
    22741 agcgactgcg tgctcgaaag gagttatcag tggaggaaat agttttcggt aacgttgttt
    22801 tgcccttcat cgtcagtcgg taacagcagt tgatgggcat tagcttcaag aatgaccatc
    22861 gaaatctgct cttcgctctg acgcacgaac catgcaaact gctcataggt tacgccctgc
    22921 ataacggata aagactggca aaccaccagc tttggcagat tatcatcctg catatcaaga
    22981 aatgctttca cggtcagcga actggcattg atggcagata aatccgccgc cagcggcaat
    23041 acggctgaag gtcggacttc cgccatggca gaaaacagga tcgtgttatc gatcagatcg
    23101 attttggcat caaagacacc gtcgaaattc tgcatatggg gcagatgcag agcctgacag
    23161 ttatcacatt caaaaaaact catccccagg tcatcgagcc attgacgcag cgtatccaga
    23221 ccaggaacga ccagcgatgt cataattttg tacaacctct ttcgataaaa agaccggcac
    23281 agcttacgca aaaagcgcag gcaaaaccat gatcagtaat gtgattgcga ctaaccaccc
    23341 gttttcaggc aatattctgt cgtagcgtgg cgttcgatcc agcggatcat tttacccgcg
    23401 atgtcgatac cggtggtttt ttctattcct tccagccccg gcgacgcatt cacctccatc
    23461 accaacggcc cgcgattagc acgcagaata tccacaccag caacgtccag cgccatcgtt
    23521 cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga tactggcgac gcttgccgcg
    23581 ccgccacgat gcaaattgga acgaaaatcg ccctctttcg cccgccgttc aatcgcagcg
    23641 accacttcat cgccaacaac cagacagcgg atatcgcacc cttgcgcctc tttgatatat
    23701 tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt caatcacgct ttccgccgcc
    23761 tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc cttcaaccaa cttgaccacc
    23821 agcggcgcac caccgaccat gtcgattaaa tcgctggtat catccggcga atgcgcaatg
    23881 cccgtgacag gcaggtcgat gccctgacgc gccagcagtt gcatggaacg caatttgtca
    23941 cgcgcccggg caatggcgac cgactcattg agcggatagc tccccagcat ctcgaactgg
    24001 cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa tacgcgggat cactgcgtca
    24061 aaatggggta acttgcggcc tttgtagtga atagaagacg ccgcaggatt tatgttcatg
    24121 tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc gctgtatagc ggcttcacgc
    24181 agccgcttac acgaatagag cgttccatcc cgggacaata tggcaatttt caccctgcac
    24241 ctctctgtca gacctggtca aaagcccgcg tatcatacat cgacgtggcg gttttagcgc
    24301 gtcgcccaac cctgtttgtg caaataatcc agaataaatg ggcggctttc tttaatgatt
    24361 gttcggcgga tatgatcgct ccaggtatcc cggcgattat tgctgccacg ggtgaggtaa
    24421 tattccgcca gttgctcgtc atactgcgcc agtgcgcctt tatccagcgg ttgatagctg
    24481 ttttcatgca ccaaaatgga ggccggtaaa cgcggcttaa gatccggatt atccgcaggc
    24541 cagccaaggc acagcccaaa cagcggcaga acatgctgcg gtaatttaag cagtttcgtc
    24601 accgcttcaa tattattgcg caggccgccg atatataccc cgcccaatcc cagcgattcc
    24661 gctgcgatta atgcattctg cgccatcatt gccgtatcaa cgacaccgag caacagttgt
    24721 tccgccaggc cgagctgagc atccggacag atctgtaaat ggcggttaaa gtcggcacag
    24781 aacacccaga actccgccgc ttgcgctacg tgtttttgcc cgccggtcag cgtcaccagt
    24841 tcttcacgta acgctttgtc ggtaatgcga ataatgctac tgcactgcaa aaaactggaa
    24901 ctggacgtcg cacgggcgct gttaataatc gcctcacgct gcgcttcgga aatgggttca
    24961 tcagtgaaat ggcgaatgga gcgatggcca caaataagtt caatggttgg cgtcattatc
    25021 tttttctctt tctgaacgtg aatattgcgg tggacggttc atcagctgtg gggcaagacg
    25081 ttttgccacc tgaagaataa ccaccaccgc agcgggaagc atgagcaaaa caccgagaaa
    25141 aatcatcaga atctgcactt ctggccgaga aaatggctca ggcagcgaca gggagtcgct
    25201 taccgacagc agcgccaccg ccagtagcat cattccgata aattccagta tcaacacgcc
    25261 tttaggcaat ttaccgatcg cgcgcatacg cttccctctg caaagtgagc cttcagtcta
    25321 aaacttttca ctgtattgtg tttaacagtt atagctttta gcaattaatg caacaggtta
    25381 aacctacttt cagcgaatac attttagcgt gatcattaca ggcataaatc tatgaggaga
    25441 gaaataatgc aaaccgttat ttttggtcgt tcgggttgcc cttactgtgt gcgtgcaaaa
    25501 gatctggctg agaaattgag caatgaacgc gatgattttc agtatcagta tgtagatatt
    25561 cgtgcggaag ggatcactaa agaagatcta caacaaaagg caggtaaacc cgtagaaacc
    25621 gtgccgcaga tttttgtcga tcagcaacat atcggcggct ataccgattt tgctgcatgg
    25681 gtgaaagaaa atctggacgc ctgatcgtct gacaagccct cgcgttgagg gctttactga
    25741 ttttttctgt gctgtggttt aaacaaacta ctgataaata agaaacacag tgcccccagc
    25801 gcacaccaga acaccgcgct tagtaaccat gccagctctt gccagaatga gcgcgtcggt
    25861 gaaaaaaaca gccgcataat gagcattgaa cagggtgccg ccagcattgc gccaaataga
    25921 ggtttcagga cttctctacg ctgtgaaaag aagctggcga ctgctccagg aagaatgaaa
    25981 aatagcaagc cgatttcagg atgcccggca gcccgaaaag cgcctttcat gtgcgtcgcc
    26041 agaaaaaggc acaccacaat gaagaggaca aaacagcaga ttgcccccgc ccaacgttgt
    26101 ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa cacatttttc gccagtgtgg
    26161 cgttcagtaa gataaagccg cttcgcattc catgctaata taggccaacg caattcatat
    26221 agccgttgat acctaatgtg attacactag taaaatatat tgttacttta ctatcgttta
    26281 ggtgcgctga atgaatctgc gccctgaatt ctggtaaaaa acattatcgt aaattaccat
    26341 ttctttcaac agcttactag taaacaagaa gttagcctcc gtgaatataa acgtcgccga
    26401 attgttaaat gggaattaca ttctgttatt atttgtggtc ctcgcgcttg ggctatgtct
    26461 cggaaagtta cgacttggtt cgatccaact gggtaattcc attggcgttt tagtcgtatc
    26521 gctgttatta ggccaacaac atttcagcat taacaccgat gcgcttaatc ttggctttat
    26581 gctgtttatt ttctgcgtcg gggtcgaagc cggaccgaac tttttttcca ttttttttcg
    26641 cgatgggaaa aattacctaa tgttagcact ggtgatggtt ggcagtgcgc tggtgatcgc
    26701 cttagggtta ggtaagctgt ttggctggga tattggcctg acggccggta tgttagcagg
    26761 ctctatgacg tcgacaccgg ttctggtcgg tgctggcgat acactgcgtc attccggcat
    26821 ggaaagcagg cagctctcac tggcactgga taatctgagc ctcgggtatg ccttaaccta
    26881 tttaatcggt ctggtgagtt tgattgttgg tgcgcgttac ttgccgaaat tgcagcatca
    26941 ggacttacag accagcgccc agcaaatcgc ccgcgaacgt ggcctggaca ctgatgccaa
    27001 ccgtaaggtt tatttaccgg tgatccgcgc ctatcgcgtc ggcccggagc tggtggcctg
    27061 gaccgacggc aaaaatctgc gtgaactggg tatttatcga caaaccggct gctacattga
    27121 acgtattcga cgtaacggga ttctggcaaa tccagacggt gatgccgtgc tacaaatggg
    27181 cgatgaaata gcgttggtag gctatcccga cgcccatgcc cgactcgatc ccagcttccg
    27241 taacggtaaa gaagttttcg atcgtgacct tctcgacatg cgtatcgtca ctgaagaagt
    27301 ggtcgttaaa aaccataacg ctgtaggtaa acgtctcgca caactgaagt tgaccgatca
    27361 cggttgcttc cttaaccgcg tcattcgtag ccagattgag atgccgatag atgacaacgt
    27421 cgtgcttaac aaaggtgacg ttttacaagt cagcggtgat gcccgtcgcg taaaaaccat
    27481 cgccgatcgc atcggcttta tctcgattca cagccaggtc actgacctgc tggcattttg
    27541 cgccttcttt gttattgggc tgatgatcgg gatgatcacc ttccagttca gcacattcag
    27601 tttcggcatg gggaacgctg ccgggttgtt attcgccgga attatgctgg gctttatgcg
    27661 tgctaaccac ccgaccttcg gttacattcc gcaaggtgca ttaagcatgg tgaaagagtt
    27721 cggcttgatg gtgtttatgg caggcgttgg tctgagcgcc ggtagcggta ttaataacgg
    27781 cctgggcgcg attggcggtc agatgttgat tgccggatta attgtcagtc ttgtgcccgt
    27841 ggttatctgt ttcttgttcg gtgcttatgt attgcgaatg aaccgcgcac tgttgttcgg
    27901 cgcaatgatg ggcgcacgca cctgcgcgcc ggcaatggag atcatcagtg atacagctcg
    27961 cagtaacatc cctgcgctgg gctatgcggg cacctacgca atcgccaacg tcctgctgac
    28021 gctggcaggg acaatcatcg tcatggtatg gccaggatta ggataaaact gaagttgccc
    28081 tgaaaatgaa atttttttgc acaaccgcag aacttttccg cagggcatca gtcttaatta
    28141 gtgccactgc ttttctttga tgtccccatt ttgtggagcc catcaacccc gccatttcgg
    28201 ttcaaggttg atgggttttt tgttatctaa aatttatcta ctaccctgca accctctcaa
    28261 ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc ggtgacaaaa tgcagcgtca
    28321 tcccctcaat aaacgcatca agcgcgcggg cggttccggg ttcaaaccat tgttcgagcg
    28381 tttgctgact gcgctgcatc cagttttgca ttaccgtttt taatagcggt tttcggctag
    28441 ccagcgcgta gagctggtac atcagctcca tgttatccgg cgttgcaacc tgtgagctgt
    28501 agatcatatc ggtgatagcc tggcatgcgc cttgagcatc actaacatcg ctaaaaaatg
    28561 cctgatattg ccgggacata atctcagtaa aactgctgaa cgcctccagc aacaactcat
    28621 caattcctga aaaatagtag gtcatcgatc ccaacggtac cccggcaagg gtagcaattt
    28681 tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc ctccagcgtg gcctggataa
    28741 ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc atttcctctg tctgcaattg
    28801 tgtacaaatg tacacaacct tgctaatgtt gtcttccctc ttcttatttt gacgtgatct
    28861 atgaccgtaa attcttcacg taatgcattg aaacgccgaa cctgggcgct gtttatgttc
    28921 ttctttttgc cgggcctgtt aatggcgtcc tgggcaaccc gtacgcctgc tatccgcgat
    28981 attctctctg tctcgatcgc tgaaatgggc ggtgttctct ttggtctgtc gatcggttcg
    29041 atgagcggta ttctctgctc ggcgtggtta gtgaaacgct ttgggacacg taatgtcatc
    29101 ctggtcacga tgtcctgcgc attgatcggg atgatgatat taagtctggc actctggctg
    29161 acatcacccc tgctcttcgc ggttggcctc ggcgtctttg gggcaagttt tggttctgcg
    29221 gaagtggcga taaacgtcga aggtgccgcc gttgagcgag aaatgaataa aacggttttg
    29281 ccgatgatgc acggttttta tagcctgggc acgctggcag gcgctggtgt cgggatggca
    29341 ctgacggcct ttggcgttcc ggcaacggtg cacattttat tggcggcgct ggtaggaatc
    29401 gcgcctattt atatcgccat tcaggcaatc cctgacggta cgggcaaaaa tgctgccgat
    29461 ggcacccagc atggtgaaaa aggcgtacct ttttatcgcg atatccagtt gctgctgatt
    29521 ggtgttgtgg tgctggcgat ggcctttgcc gaaggttctg ccaacgactg gttaccctta
    29581 ttaatggttg acggtcacgg ttttagccct acttccggct cgctgattta tgccggtttt
    29641 accctgggga tgactgttgg acgctttacc ggcggttggt tcatcgaccg ttacagtcgc
    29701 gttgccgtgg ttcgggccag tgcactaatg ggagcgttgg gtattgggct gattattttt
    29761 gtcgatagcg cctgggtcgc tggggtgtct gttgtactct ggggactggg tgcctcgctg
    29821 ggcttcccgc tgaccatttc tgccgccagc gataccggcc ccgatgcacc aacccgcgtc
    29881 agtgtggtag caacgaccgg ttatctggct ttcctcgtcg ggccgccgct gctgggctat
    29941 ctcggcgaac attatggatt acgtagtgca atgctggttg tactggcgct ggttattctc
    30001 gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca aaacgcagac ggcgatggag
    30061 aatagttgat gagcattaaa ttaattgcgg tagacatgga tggtactttc ttaagcgatc
    30121 aaaaaaccta taaccgtgag cggtttatgg ctcagtatca gcaaatgaaa gcacaaggca
    30181 ttcgctttgt ggtcgccagc gggaatcaat attatcagtt gatctctttc ttccctgaaa
    30241 ttgcgaatga aattgccttt gtggctgaaa acggcggctg ggtagtgagt gaaggcaaag
    30301 atgtttttaa tggcgagctg tcgaaggatg cgtttgctac tgtcgtggaa catttgctga
    30361 cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag cgcctataca ctcaaaaaat
    30421 atgacgatgc catgaaaacg gtggcggaaa tgtattatca ccgtctggaa tacgtcgata
    30481 actttgacaa cttagaagat attttcttta agtttggcct gaatctttcc gatgaactga
    30541 ttccacaagt acaaaaagca ttacatgagg ccatcggcga tattatggtg ccagtccaca
    30601 ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca taaagccaat ggccttcgcc
    30661 aactgcagaa attatgggga atagacgaca gcgaagtggt ggtctttggc gatggcggta
    30721 acgatattga gatgctgcgt caggcaggct ttagttttgc aatggaaaat gccggcagcg
    30781 cggtcgtcgc agcggcaaaa taccgcgcag gctccaataa ccgtgaaggc gtactggatg
    30841 tgatcgataa agttcttaaa cacgaagcgc catttgacca ataaaaacgg cagcacgccc
    30901 ggcaacacaa tgcctgaatg aaggctgcat aattcgcgga aattaacccg ctgcattatt
    30961 tcaggcgaaa tgattatgaa aaattgtcta ctgttgggcg cgcttttaat gggctttact
    31021 ggtgtggcga tggcgcaaag tgtcaccgtc gatgtgccga gtggatacaa agtggttgtg
    31081 gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg cgactgtgcc gcaaaccgtc
    31141 tatgtggctc ctgcgccagc tccggcttac cgcccgcatc cttatgttcg ccatcttgcc
    31201 agcgtaggtg aagggatggt tatcgaacac cagattgacg accatcacca ttaattcgag
    31261 aatgcctgat cgcacaaagc aatcaggcat ttttttaccc ttcgtgagaa tttcccatct
    31321 gtttatcttt taaaaagata accatcagcg acagccacaa aattccgttg acaaggttga
    31381 agagattaaa cagtccgttg cccccgttca gccaggcatg tttgctgatt tcaataccga
    31441 cggtaaagat cagcatttgc agcattccca tcgccgcaga aaccgtacct ttactcatat
    31501 cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc cagaccaatg ccgaaagcat
    31561 aaatacttaa cccggcggtc atccataaat acgcgtgcga tgagataacc gttgccgcag
    31621 cagcgaccaa cagaccaatc ataatcggcc agccgcccat aattatcagc gaacgtacgg
    31681 tgcggcgcga ggtcaaacgc gctaacaaca agttacccgc aattaacgcc ccgaaaatag
    31741 gcacttgcag caagccatat tcatagctgc tcaactgctc gccggtaatg atgataatcg
    31801 gcgactgggc gatccacgcc agcaatggca ggctaacgaa tcccagcgcc agcgcccccg
    31861 ccacaaagcg gccgttcttc agcaccagct tatagtcacg accgagttct ttcagtgaca
    31921 gtttctcgcc tatacgcgtg gcggtttcag gcatggcttg ttgcagaccg aaaaaggaga
    31981 tcgctgccaa tgcggcaaac aagacaaaca tcccttccca gggcagcaca tggatccacg
    32041 ccgcgcccac cagcggacca agtagcggag caatcagcgc cacgttcgcc atcagcgcgg
    32101 tgatcttgat acaaaccgcc tcttcgaagg attcctgaat tgcggcgtat cccacagcgc
    32161 caatgaaaca gaggcttatg ccctgcaaga agcgcaacag agtgaattgt tcaatgtttt
    32221 gcgccagtaa tattgccaga caggtgatga taaaccacac cacccccgcc agcatcaccg
    32281 gacggcgacc aatacgatcc gacagcggcc ccaggagcca ttgtaaaaac atcccgcccg
    32341 ccagatatgc ggtcattgaa gtaggaaccc aatcaatgcc cgcctgatat tgttccacca
    32401 cggccaacat accgggttga atcatatcgt tgccgatata ggttgaaaat tcgtaaagca
    32461 ccagacagag agggaaaagt aacgcctgac gtccaagcct ggcaccggaa gctaatttat
    32521 tttgcatgca atttcttcgc caataataat cgcgcagagt ttaataaaag cacagctaac
    32581 gagaaagcga attttgtagc tgaaaccacg gttaagcaca ttcttacatt attgcgagta
    32641 tagctacgct ttctttaagt tttatttaac ctatgcccgt tacaattacc caccgtaaac
    32701 aggccgcttg agggaaataa gacgatgccg ctttacccag tttaacctgc actttattct
    32761 caacgacttg cctgtattgg ctccctttta atcactttgc gtcgggaagt tatgctggaa
    32821 aatttgaatc tctctctatt ctctcttatt aacgcgacgc cagactcggc tccgtggatg
    32881 atctcgttgg cgatttttat tgctaaagat ttgattaccg tggtaccgct gctggccgcg
    32941 gtgctttggt tgtgggggct tacagcacaa cggcaactgg tgataaaaat cgctatcgcg
    33001 ctggcggtca gcctgtttgt gtcctggacg atgggacatc tttttccgca cgaccgaccc
    33061 tttgtcgaaa atatcggcta taacttcctg catcatgcgg cggatgactc attcccaagc
    33121 gaccacggta cggtgatttt cacctttgca ctggcatttt tatgctggca tcgcctgtgg
    33181 tccggctcac ttttaatggt gctggccgtc gtcattgcct ggtcgcgcgt ttatcttggc
    33241 gtgcactggc cgctggatat gctcggtgga ttgctggctg gtatgattgg ctgccttagc
    33301 gctcagatta tctggcaagc gatggggcat aaactctatc aacgtctgca atcgtggtat
    33361 cgcttctgtt ttgcattacc gatccgcaaa ggctgggtgc gtgactgatc tctgctaaaa
    33421 agtgtagtat tgagcggctc gcttcaataa ctattcagag ggattatgga aacacgtcgc
    33481 gaagagcgca tcgggcagct gctgcaagaa ttaaaacgca gcgataagtt acatcttaaa
    33541 gacgccgccg ccctgcttgg ggtttcggag atgacgattc gtcgcgatct gaacaaccac
    33601 agtgcgcccg tcgttttgct cggcggctat attgttctgg aaccgcgcag tgccagccat
    33661 tacctgttaa gcgatcaaaa atcccgcctg gtggaagaaa aacgccgggc ggcaaaaatg
    33721 gctgcgacac tggtagaacc cgatcagact ctcttttttg actgtggcac caccacgccg
    33781 tggattattg aagcgattga taatgaaatc ccttttaccg ccgtttgtta ttcgctaaat
    33841 acctttctgg cgctgaaaga gaaaccccat tgccgcgcat ttctgtgcgg tggtgaattt
    33901 cacgccagca acgccatttt caaacccatc gattttcagc aaacgttgaa taatttttgc
    33961 ccggatatcg ctttttattc cgcggcgggc gtgcatgtca gtaaaggcgc tacctgtttt
    34021 aatcttgaag agttgccggt aaaacactgg gccatgtcga tggcgcaaaa gcatgtgctg
    34081 gttgtcgacc acagtaaatt tggcaaggtg cgtccggcgc gcatgggtga cctgaaacgc
    34141 tttgatattg tggtgagtga ttgttgcccg gaagatgagt atgtgaagta cgcgcagacg
    34201 caccgtatta agttgatgta ttaatgacgt ataaccggat gacgtttcgc gccatccggt
    34261 tatcagaaga ttaagagaac cagctgccga accactgatg gaatttcatc atcacgaaat
    34321 cccacatccg accaaagaat ccgccctctt ccacattttc catcacgatc agcggacgct
    34381 gctcaatgga tttaccgtta agctggaaat caatggtccc gacaacctga ccttttttca
    34441 gcggtgcggt aagctgcggt tccgttaacg tgtagctcgc cttcaggttt ttcagctgcc
    34501 cacgcggtat ggtcactgag cccgcttcgc ctgccccaag attcacttcg ctcttatcac
    34561 caaaccagac gcgctgagtc acaaaggtgg catcaggttt aattggcgtc acggtttcaa
    34621 agaagcggaa accccaggtc aataatttct cagactcatt aaaacggata cggtcagttt
    34681 tcgcccccag cactacggag attaaacgca tatcgccctg ggtagccgaa gcaaccagat
    34741 tatatcccgc gcctgccgtg gttcctgtct tcatgccatc aacattcaga ttgctgctcc
    34801 acagcagacg gttacggtta ggctgacgaa ttttgttgaa ggtgaactct ttctctttat
    34861 gaatggcgta ctcttccggc acatcgtgga tcaatgcttt acccagcaat gccatatcgc
    34921 gcgcggtact gaactgcccc ggcgcatcca gaccgtgcac cgtctggaag gtagtgttgg
    34981 tcagacccag ttttttggca taaccattca tcaaaccaat gaatgactcc tggctcccgg
    35041 caacgtaatc ggccagcgca atacaggcgt cattaccgga ctggataatc acacctttgt
    35101 tcaagtctgc caccgaaacc tgatcgcccg gtttgaggaa cattaccgat gaaccacgca
    35161 gtgccggatt tcccgtcgcc caggcatctt taccgaccgt caccatatcg gtgagtttaa
    35221 tcttatcggc cttaagcgcc tgcccaacca catagctggt catgatttta gtcaggctcg
    35281 cgggatccag tttctcatcc gcgttgcctt ctgccagcac tttaccgctg gcgtaatcca
    35341 ttaaaatcca tgcacgcgca tccacgctcg gcgcttcaac ggtttgttcc gccgcgaatg
    35401 ccgttggggc aaaaaggaat aaaaatgcag aacccgctgc aagaccacga aggagagagg
    35461 agtattgcgt cataaatgcc acccgagtat ccattcaaaa aaaatacgtt atatcgccgt
    35521 atgataagtt cggtgattaa taacgcacac acaaacttaa agaaaccgta agttggtaaa
    35581 gtttttaaag tttatgcaat atcctgccgc ttcgctgctt ctggtgtcat ttttttgcca
    35641 gctgttgctt aaaagttaac tttacttcac catcggagtc ctgttcagcc tgtggctgcc
    35701 tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg aataattcaa ccaacgtaaa
    35761 aaaagtattg ctgacgctcg aagaactgga actaccttat gagcaaattc tcgcgggccg
    35821 tgagtttggg ataaatcacg atgctgattt tctggcgatg aaccctaacg ggctggtgcc
    35881 gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa tcaaacgcca ttgtccgcta
    35941 tctggcggca cagtacgggc aaaaacgtct gtggatcgac tcaccggcac gtcgtgcgga
    36001 agcggaaaaa tggatggact gggcaaacca gacgctcagt aatgctcatc gcgggatcct
    36061 gatgggatta gtcagaacac caccggaaga gcgcgatcag gccgccattg atgccagttg
    36121 caaagagtgc gacgccctgt ttgccctgct cgatgcggaa ctggcaaaag taaaatggtt
    36181 ctctggcgac gagtttggtg tgggcgatat cgctatcgca ccgttcatct acaatttgtt
    36241 taacgttggc ctgacctgga caccgcgtcc aaatctgcaa cgctggtatc agcaactcac
    36301 tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt agctaattgc gtgggctaac
    36361 tttaagtaat tccccactgg actcgtcggt gagaacgtat aaataaccgt cgggtccagt
    36421 gcgaacatca cgaattcgct gccctctgtc cgttaaaata cggccatctt ctgtcacttt
    36481 gtcgccgttg acgctcatca caatgacatc tttatctttc agcgcgccaa taaataattt
    36541 ttgctgccac tgggggaatt tatcgctgtt atagaaggcc atgccgctca cagcgggcga
    36601 atctttccag taaaaaacag gttgctcggt cccggcgacg atctcccctt tcgcttccgg
    36661 tatcttaaag cctgaatagt tgattcccca ggttgccagc ggccagccgt agtttttgcc
    36721 tttttgcggg atattaattt catcaccacc gcgcgggcca tgttcattca gccacagtgc
    36781 attactccac ggattcattg ccattccttg cggattacga atgccataag accagatctc
    36841 ggcgcgcaca ccggattcct ttataaaagg attatcatcc gggatttcgc cctggtcggt
    36901 cagacgcacc agtttgccct gtaatttatc cagatcctgc gccgtcgggc gctgattgtt
    36961 ttcgcccaga gcaataaaaa gataaccttt accgtcgaat accagccgcc cgccaaaatg
    37021 gttgccggta gacagttttg gcatctggcg gaagacggtg cggaagtcgg tcacttttga
    37081 gagatcatca cttaagcggc cataacccac agcagttccg gctttgccat catcgccaac
    37141 ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc
    37201 cagcaggccg ccctgcccgt gcgcccaaac gtccggaact ccggaaagcg gcgcagataa
    37261 tccttttcct gcttgccagt gacgcaactc gccgccgcgc agagtgatta acataccgtg
    37321 attatcgggt aaaaaggcca gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac
    37381 atttaccgtt gcaggagccg cccagagagc ggaagaaaga caaataaggg gcacaaggaa
    37441 aaaggattgt cgatgcataa cgcctccgga ttgactctgg cttaaagcgt agtcagtgga
    37501 ggagataaca aattcatttt tacaaaaact taaacatgaa gggggagacg ctttctcccc
    37561 cttagttttc aggccttctc aagcatggcg tgcttctgca ggctctggat actcagcgtt
    37621 aagctcatca gacaattttc aagcttatcg gcgttgacgg taataacagt cgggcaatca
    37681 tggtgcccac tcatcaaaca tactgcggct gtcgctaatg cttcttcagc atgatgaaga
    37741 gcactccact cttcctgatc cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg
    37801 cgattcagtt taaaaaagtt atctcgtaga tgattgcttt cgctgacgga catgtatcct
    37861 tttgccttcc tcagttgcac gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc
    37921 agcagatcga ttcgctgtct gtcaacgaac atacttcgat cctcctcttt ccagccttgt
    37981 tgcaattaaa tagtatggct attttttgag caaaatgctg tccaggatca attcttcacg
    38041 ccgaattaac aaaatttaca tttccttcgc ctccgccagc gaatgagatt gcgatacaaa
    38101 ttttatccag aactctgtag aatccctgcc ctgacctttc ctgtaactga atatttttta
    38161 agctatgagc aaagtaactc cccagccgaa aatcggcttt gtttcccttg gctgtccgaa
    38221 aaaccttgtc gattcagagc gtattctcac cgaactacgc actgaaggtt atgacgtggt
    38281 accgagctat gacgatgcgg acatggtgat cgtcaacacc tgcggcttta ttgacagcgc
    38341 ggtacaagaa tcactggaag ccattggtga agcgttgaat gaaaacggca aggtaattgt
    38401 gaccggttgt ctgggggcaa aagaagatca gatccgcgaa gtccacccga aagtgctgga
    38461 aatcaccggg cctcatagct atgagcaggt tctggagcac gttcatcact acgtgccaaa
    38521 accgaaacac aacccattcc tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg
    38581 tcattatgcc tatctgaaaa tttcggaagg ctgtaatcac cgctgcacct tctgcattat
    38641 tccgtctatg cgcggcgacc tggtgagccg tccgattggc gaagtgttaa gtgaagcgaa
    38701 acgtctggta gatgcgggcg ttaaagagat tctggtgatc tcgcaggata cttctgccta
    38761 tggcgttgat gttaaacatc gtactggctt ccacaacggc gagccggtaa aaaccagcat
    38821 ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg acacgtctgc actacgttta
    38881 cccttatccg catgtggacg acgtcatccc actgatggca gaaggcaaaa tcctgccgta
    38941 tctggacatt ccgttgcagc acgccagccc gcgcattctc aaactgatga agcgtccggg
    39001 ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac
    39061 cctacgctca acctttattg tcggcttccc tggcgagaca gaagaagatt tccagatgct
    39121 actcgacttc ctgaaagaag cgcgtctgga tcgcgttggc tgctttaaat acagcccggt
    39181 tgaaggtgca gacgccaatg ccctgcctga ccaggttccg gaagaagtta aagaagaacg
    39241 ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc gagcgcctgc aagagaaagt
    39301 gggccgtgaa attctggtga ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag
    39361 catggcagat gcaccggaaa tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa
    39421 gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat gagtacgatt tgtggggtag
    39481 ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac
    39541 tgccagctaa tctgagccct gccgtttttc ttactcttat acagccgctc atccgccagc
    39601 attttcaagc gttcgacggt ggtacattca tttgaatgcg ctacaccgat actggtactt
    39661 aaattaatgc ttcccacctg gaaatgaacg atttctttta gaatcgatag caactcttcc
    39721 agcgttccac ccgtataaat caccgcaaat tcgtcaccgc cgaagcggta gattttacct
    39781 ttatctaccg ggatctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc
    39841 agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa caggcacaaa
    39901 taaccgcctt tttccggcag cgcttttaaa tcttcgtcaa aggattttct gcccattgct
    39961 ttggtcaggt cgtccaggaa cgccgagtta tacatactgt agagtttttt gacatcggca
    40021 gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat ggttattaat gatggcttct
    40081 ttctccacaa taccacgatt aatcactctg cgtaccagat agaggcaact ggtacccgat
    40141 atcactgcca ccaatgcggc aaagaaaata atcaatgtac agacccgcaa ataaacatct
    40201 tttttatacg caaatacgta caatccggtc ggcaaatagg cgaagtgctc catcgcttcc
    40261 gaaacggtga agatggcgtt tttagcataa tattcatcac cggtcaccgg tttaccgttc
    40321 ttaaatagag agaacatcag gttctcgctg tccatcgcca gcgattttga cagtaaagaa
    40381 aaacgctcca gaccaagcaa aaatatcaga ctacgattgt gagttaccgg atagaggtaa
    40441 tagacgaagg ttttaccgtc aaaagagata aaccctgtat ttttgacatg tcgatacttc
    40501 acaacctgca taatatctgc acaataattt ttctcgaact cctttacctg aatacattcg
    40561 tcactggaaa tcagataagg accgacatcg tacaaataag agggggaaac cgagtcggcg
    40621 taagctgtgc gcaaaccaac caacagctgt tcctccgtgg gatttagcgt gttgataaga
    40681 tcaattgcca cgctacgccg atattcagca atatttgcca ccgcaaaagc a
//
LOCUS       DAZKGS010000022        39908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKGS010000022 DAZKGS010000000
VERSION     DAZKGS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..252)
                     /gene="chbB"
                     /locus_tag="ACHOQY_001216"
     CDS             complement(<1..252)
                     /gene="chbB"
                     /locus_tag="ACHOQY_001216"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9677357.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLY"
     gene            complement(551..889)
                     /gene="osmE"
                     /locus_tag="ACHOQY_001217"
     CDS             complement(551..889)
                     /gene="osmE"
                     /locus_tag="ACHOQY_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9677358.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1091..1918
                     /gene="nadE"
                     /locus_tag="ACHOQY_001218"
     CDS             1091..1918
                     /gene="nadE"
                     /locus_tag="ACHOQY_001218"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9677359.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2148..3035
                     /gene="cho"
                     /locus_tag="ACHOQY_001219"
     CDS             2148..3035
                     /gene="cho"
                     /locus_tag="ACHOQY_001219"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP9677360.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2995..3570)
                     /gene="ves"
                     /locus_tag="ACHOQY_001220"
     CDS             complement(2995..3570)
                     /gene="ves"
                     /locus_tag="ACHOQY_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP9677361.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3773..4258)
                     /gene="spy"
                     /locus_tag="ACHOQY_001221"
     CDS             complement(3773..4258)
                     /gene="spy"
                     /locus_tag="ACHOQY_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9677362.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4588..5556)
                     /gene="astE"
                     /locus_tag="ACHOQY_001222"
     CDS             complement(4588..5556)
                     /gene="astE"
                     /locus_tag="ACHOQY_001222"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9677363.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5549..6892)
                     /gene="astB"
                     /locus_tag="ACHOQY_001223"
     CDS             complement(5549..6892)
                     /gene="astB"
                     /locus_tag="ACHOQY_001223"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9677364.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6889..8367)
                     /gene="astD"
                     /locus_tag="ACHOQY_001224"
     CDS             complement(6889..8367)
                     /gene="astD"
                     /locus_tag="ACHOQY_001224"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9677365.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8364..9398)
                     /gene="astA"
                     /locus_tag="ACHOQY_001225"
     CDS             complement(8364..9398)
                     /gene="astA"
                     /locus_tag="ACHOQY_001225"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9677366.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9395..10615)
                     /gene="astC"
                     /locus_tag="ACHOQY_001226"
     CDS             complement(9395..10615)
                     /gene="astC"
                     /locus_tag="ACHOQY_001226"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9677367.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11061..11867
                     /gene="xthA"
                     /locus_tag="ACHOQY_001227"
     CDS             11061..11867
                     /gene="xthA"
                     /locus_tag="ACHOQY_001227"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9677368.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12034..12744
                     /locus_tag="ACHOQY_001228"
     CDS             12034..12744
                     /locus_tag="ACHOQY_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9677369.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12749..13426
                     /gene="ydjY"
                     /locus_tag="ACHOQY_001229"
     CDS             12749..13426
                     /gene="ydjY"
                     /locus_tag="ACHOQY_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9677370.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13441..14148
                     /locus_tag="ACHOQY_001230"
     CDS             13441..14148
                     /locus_tag="ACHOQY_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9677371.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14148..14696
                     /locus_tag="ACHOQY_001231"
     CDS             14148..14696
                     /locus_tag="ACHOQY_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9677372.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14706..15872
                     /locus_tag="ACHOQY_001232"
     CDS             14706..15872
                     /locus_tag="ACHOQY_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9677373.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15845..17380
                     /locus_tag="ACHOQY_001233"
     CDS             15845..17380
                     /locus_tag="ACHOQY_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9677374.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17380..18033
                     /locus_tag="ACHOQY_001234"
     CDS             17380..18033
                     /locus_tag="ACHOQY_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9677375.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            18100..19407
                     /gene="ynjE"
                     /locus_tag="ACHOQY_001235"
     CDS             18100..19407
                     /gene="ynjE"
                     /locus_tag="ACHOQY_001235"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9677376.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19416..20036)
                     /locus_tag="ACHOQY_001236"
     CDS             complement(19416..20036)
                     /locus_tag="ACHOQY_001236"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9677377.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20123..20530
                     /gene="nudG"
                     /locus_tag="ACHOQY_001237"
     CDS             20123..20530
                     /gene="nudG"
                     /locus_tag="ACHOQY_001237"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9677378.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20496..20768)
                     /locus_tag="ACHOQY_001238"
     CDS             complement(20496..20768)
                     /locus_tag="ACHOQY_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP9677379.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21004..22347
                     /gene="gdhA"
                     /locus_tag="ACHOQY_001239"
     CDS             21004..22347
                     /gene="gdhA"
                     /locus_tag="ACHOQY_001239"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9677380.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22464..23504)
                     /locus_tag="ACHOQY_001240"
     CDS             complement(22464..23504)
                     /locus_tag="ACHOQY_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9677381.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23632..25593)
                     /gene="topB"
                     /locus_tag="ACHOQY_001241"
     CDS             complement(23632..25593)
                     /gene="topB"
                     /locus_tag="ACHOQY_001241"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9677382.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGMPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(25598..26641)
                     /gene="selD"
                     /locus_tag="ACHOQY_001242"
     CDS             complement(25598..26641)
                     /gene="selD"
                     /locus_tag="ACHOQY_001242"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9677383.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(26758..27309)
                     /locus_tag="ACHOQY_001243"
     CDS             complement(26758..27309)
                     /locus_tag="ACHOQY_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9677384.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27470..29326
                     /gene="sppA"
                     /locus_tag="ACHOQY_001244"
     CDS             27470..29326
                     /gene="sppA"
                     /locus_tag="ACHOQY_001244"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9677385.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGGTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29493..30509
                     /gene="ansA"
                     /locus_tag="ACHOQY_001245"
     CDS             29493..30509
                     /gene="ansA"
                     /locus_tag="ACHOQY_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9677386.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30520..31161
                     /gene="pncA"
                     /locus_tag="ACHOQY_001246"
     CDS             30520..31161
                     /gene="pncA"
                     /locus_tag="ACHOQY_001246"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9677387.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31322..31594)
                     /locus_tag="ACHOQY_001247"
     CDS             complement(31322..31594)
                     /locus_tag="ACHOQY_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9677388.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(31636..32049)
                     /gene="msrB"
                     /locus_tag="ACHOQY_001248"
     CDS             complement(31636..32049)
                     /gene="msrB"
                     /locus_tag="ACHOQY_001248"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9677389.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            32391..33386
                     /gene="gapA"
                     /locus_tag="ACHOQY_001249"
     CDS             32391..33386
                     /gene="gapA"
                     /locus_tag="ACHOQY_001249"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9677390.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            33470..34354
                     /locus_tag="ACHOQY_001250"
     CDS             33470..34354
                     /locus_tag="ACHOQY_001250"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9677391.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(34405..35259)
                     /gene="yeaE"
                     /locus_tag="ACHOQY_001251"
     CDS             complement(34405..35259)
                     /gene="yeaE"
                     /locus_tag="ACHOQY_001251"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9677392.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(35349..36095)
                     /gene="mipA"
                     /locus_tag="ACHOQY_001252"
     CDS             complement(35349..36095)
                     /gene="mipA"
                     /locus_tag="ACHOQY_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9677393.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            36531..38465
                     /gene="yeaG"
                     /locus_tag="ACHOQY_001253"
     CDS             36531..38465
                     /gene="yeaG"
                     /locus_tag="ACHOQY_001253"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9677394.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            38578..39861
                     /locus_tag="ACHOQY_001254"
     CDS             38578..39861
                     /locus_tag="ACHOQY_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9677395.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         9653 a        10469 c        10039 g         9747 t
ORIGIN      
        1 ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg
       61 caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc
      121 agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc
      181 gcgcattttt gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg
      241 tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc
      301 cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg
      361 aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata aattgtttct
      421 ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta
      481 agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca
      541 gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac
      601 cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat
      661 cacgttgacc caggatgtag gtctggcaag taccgcgagc atggatcatg ctcacttcag
      721 acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt
      781 tcaccacagg ctgtacaaac tggtctttgg tacgatcata agccgtacaa cccgccagca
      841 tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc
      901 ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca agcccgtgaa
      961 cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct
     1021 gtcgcttgtc gtttcagtag caacgggtta gctttaagga agttttgtct tttctgtctg
     1081 gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat
     1141 taatgctgaa gaggaaattc gtcgtagtgt cgattttctg aaaagctacc tgcaaactta
     1201 tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg
     1261 aaagctgtgc cagatggcga ttaatgagct gcgccaggaa actggcaacg aatcactgca
     1321 atttattgcc gtacgcctgc cctatggtgt tcaggctgac gaacaagatt gccaggatgc
     1381 cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc
     1441 tagcgagcag gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga
     1501 aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt
     1561 ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg
     1621 cggtacggat attaatccgc tgtatcgtct caacaaacgc cagggtaaac agttgctgac
     1681 ggcattaggt tgcccggaac acctttataa gaaagcccca acggccgatc tggaagatga
     1741 tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta
     1801 tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa
     1861 aaccgaacat aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata
     1921 atttgcacat aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa
     1981 cgccttattc gacctacaaa actatgcaaa ttcaatatat tgcaggagtc gaataggcct
     2041 gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg
     2101 cttcccgcct gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt
     2161 taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac
     2221 gttcattcct taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg
     2281 acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt
     2341 tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgca
     2401 ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc
     2461 tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc
     2521 gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc
     2581 tgtttgccaa taggcgcgca gctttgcaag cattgcagag catcgctgat gaacaaaaac
     2641 tttgttatgg cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag
     2701 cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac
     2761 gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggggcggtgg
     2821 cgctgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc
     2881 tgggggcggt taattcgctg gaagacgcga caacgttaat tcggacaccc gccgggtttg
     2941 atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg
     3001 aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg
     3061 cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt
     3121 gagcagtttg tcgcccagtt gccacgcccc gttaatcaca aacactaccc cgccgcgtga
     3181 gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg
     3241 cgtcataata ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt ttaccacctg
     3301 gtccgccgca aaggcaaaag gctgtaacgg ttttaaggta tggttaaagt ggtctgcgct
     3361 ttcaaggaac atctcgccgc cttccagcaa cgtcactatc ctttccatac cgggaaacaa
     3421 agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt
     3481 tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc acagattcac
     3541 cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt
     3601 atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc
     3661 aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat
     3721 tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag
     3781 cagttgcagg cattttacct tttgccgctg gacgttctgt cagacgcttc tcaaaattag
     3841 cattaaattg ctttttctgt tccggcgtca ggatgttgta aattttgttc tgggtttcca
     3901 tgtgcgccag catgttagct ttgcgctgtt cttccatttt tgcgatctgc gcttcagctt
     3961 ttgctttatc gaaggtatcg ctggcaacga tgtcatgcat tgcgcggcgt tcttccagcg
     4021 gcggacgttt catctggtca cgctggcctt tcatgatttc gcggatctgc tgtttctgcg
     4081 cgtcggtcag gttcaggtct ttgaacatca tgtcctgatg cggaccgaac ttgcctttgt
     4141 ggtgcataat cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg gcatgggcca
     4201 ggttagccgc gccaagagcc agggtagagg caacaaacag tgcagttaat ttacgcatat
     4261 tctatatcct tcctttcagt tatttattac ggctttctta agtagcgtgc cgtgttgacg
     4321 agattaactt tactgacttt agcgtcaatt aatcagagca acggtaaaac aatgaaagtg
     4381 taaaaaacac ttttgcgcca attatggaga aaaaaagaaa atttgatgga gagtgatgag
     4441 agaatattac aacacgatga ttttgcagag attatgaaga actataccgg atgactggtg
     4501 ataaataaag caaataacca ggattaatct gtattaattt ataagaaagc aacttaatac
     4561 ccgcagagtg atttctgcgg gtaagtatta gcttattttt tcgagcatta atcccgcgcg
     4621 taatcccaac gctaccaacg gattagggaa taacacatac tctacatcat gggttacggt
     4681 aaaacgttcc tctccgtcct gcgccagcaa tgttcctttc tcaaacggca taaaattcag
     4741 cgtgtcactt gccatatgca tttcgaagga cggcgagtgg cgagtaattt gcgaaaccac
     4801 ccgataacgg agcggcggtg ttctcacgat accgacactc tcaccagata gcagcgcagc
     4861 aattgcgctg gcagttactg caaactggcg aagatcgttt tgcccaaagg gcaacgcttt
     4921 gccaagttcc agcgtacagg ccagcgcgcc aaaatgtctg gcgctgaaat gggtaaacgt
     4981 accaccaggt tcctgatgga acaccagcgc ctccagcccc gccgcaccca gccacgtcag
     5041 aaatttctcg tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg gatgcaagga
     5101 gccacgaatt gcggtatgta gatcaaggtg ccagcgcaca gattctttgc cctggtcata
     5161 aaaatcttcc aggcactgtt ccagttcgcg cgcccgacag gtttctccgc tttcagcaaa
     5221 tagctgccaa cgaccgccaa acattcgatt catatcgcta tggcaataac gtttcccttg
     5281 cttcagcgca ggaggattcc cgaggatcac cagcaaccgc caacgtaacg ggatctcgcc
     5341 gtgagatatc gcgccaagca acgcgtccag catctccaca ggtgccgtct cattaccgtg
     5401 tattcccgct gaaatcacca gtgcgccttg cggtggcgtt aatggcgtca gttccagcac
     5461 accatcgccc agccagcgcc agcgaacgcc gttgatttct cgctcggtga taaccggttt
     5521 tttacccgtt aaggtcagag caagaaaatt atccattgcc ccctccctcg cgctggaacg
     5581 gataaaccga accgagattc agtaattggc tcaatacatc cagtgcttcc cgcccttcgc
     5641 gcagcaattg cgggtcggcc agatcggcag cagtaaggcg atcgcggtag taacgatcca
     5701 cccagtcatt gagcgcatta aacagcgtat cgttcatcat caccgccgga ttcaccgccc
     5761 ggcgttcttc ttctgtcaat accacccgca accgcaggca cgccgggccg ccgccattcg
     5821 ccatgctttc acgtaaatca aagactttta gttcgctaat cgggttgtca gcggcaagga
     5881 gttcattgag ataaccccat actccggcgt gttcccgaca ctcctgaggc agcaccaaca
     5941 tcatagaacc atcatcgcgg ctcaacagtt ggctgttaaa cagataggta gacaccgcat
     6001 ccgacacgga aacctgagtt gccggaactt ctatcgccat aaaaccgttc acccgcgcac
     6061 gcaggtttgc cagtaactgt gactggcgag cgaacgcctg ttggtggcaa aacagcacct
     6121 ggcggttact cacggcaatc acgtcattat gaaaaacgcc ctggtcgata acgtccgggt
     6181 tttgctgggc gaaaatcacc tgttggggat tcacctgatt cagccttgcc accgcctcgc
     6241 tggcttcgcg agtctgtcgc gccggataac gggaaggccg ggtatcattg ccttcttctc
     6301 gcccgtagac aaaaagttgc atacccggct caccgtaatg accgccgaga cgattgtggt
     6361 ttgccgcccc ctcatcaccg agcaacgcta cctgcggcaa cgccgaatga acgctaaatt
     6421 tctcttcgtc gttaaaaatc gcttttaaaa gtgattcggt gacgtgcgct tccagcgagc
     6481 ggtgaaattt attgttcagg ttggcaacgg tgagatgcac tttgccatcc agcgtatcgg
     6541 cagatggcgc gatcgttgcc gcattggcta cccacattgg cgaagcggaa ctgacgctgg
     6601 aaagccagtg cggtgcctgg cgtgcaactt tttccagtac ctgctcatcg ctaccactga
     6661 atcccaactg acgcagcacc ggaataaacg gacgctcgtg cggcgggatc acggcctggg
     6721 ggaatcccgc atcggcaagg gctttcattt tcagtaagcc ctgttttgcc gcctgtcgcg
     6781 ggttagacac ctgaaaacgg tgacgggtag aggcttcatt accaaacgac aggccagcgt
     6841 aatgatgcgt cagccctacc agcccgtcga aattaacttc ccaggcgttc atcgcaccac
     6901 ctcatcggaa aaatccagcc cggggttaag cgttgcgggc aaagttaacg agtccgactc
     6961 caggctcgcc ataggccatg cgcagtaatc tgcggcatac caggcgctgg ggcgatggtt
     7021 accggatgca ccaatgccgc cgaatggcgc ggtactggca gcaccagtaa gcggtttgtt
     7081 ccagttaaca atccccgccc gcgcctccag caacagttga tcgaattttt cccgctcggg
     7141 ggaaaccaga ccgcaagaga gtccgaagcg agtgttattc gccattagaa tcgcttcctc
     7201 gaaagaatca taacgccaga cgcgcaataa cggtccgaac acctcttcat ctggtacgcc
     7261 agcaacgcct gtcatttcaa tgatccccgg cgtcagcaac gatgtctctg attgtaataa
     7321 gcgcggcgca agcagggttc gtccgcccat cgcttccagt tgctgccagg cagtaaccac
     7381 ctgctgtgcg gcctgttcag aaatcagccc gccaataaac ggctgcggtt cgtcatccca
     7441 gttgcccggc gttaatcgct ggctgacggc aaccagacga gcaagaaacg catcgccctg
     7501 cgctccgctt ttgagcaata aacggcgggc gcaggtgcag cgttgcccgg ctgtgacaaa
     7561 cgccgactga atggtcagat ggacagccgc gtcgatatcc gccacctcat cgataattag
     7621 cggattatta ccgcccatct caagggcgag aattttctcc ggctgaccgg agagctggcg
     7681 atgcagctgg tagccggtat tggcgctacc ggtaaacagc aaaccgtcga gatcctccag
     7741 cgcactcagc gcctgacccg tttcacgccc gccctgcacc aggttcagta cgcccggcgg
     7801 caagccagcc tgctgccata aacgcattac cgcttcgcca ctccacggtg tcagttcgct
     7861 gggtttaaag ataatggtgt tacctgccag caatgccgga acgatatgtc cgttcggcaa
     7921 atgaccaggg aaattatacg gcccaaacac cgccagcacg ccgtgcgggc gatgtcgcag
     7981 gctcgccgca ccgtccggca tttcactacg ctgctcgccg gtacgaacgt gatacgcctt
     8041 aattgatatc gcgattttat tgatcatcgc cgtcacttcg gttgccgctt cccagcgcgg
     8101 cttacccgtt tctctggcaa taatcgcggt taattcgcct ttattccttt ccagcaatcc
     8161 ggcaaagcgt tcgacaacga cctgacgttc agccaatgag agccgcgccc agcgcggaaa
     8221 cgccgcacgg gctgcccgac aagcctgttc gacctgagcg gcatcggcat cattgccttg
     8281 ccataacacc tcgcccgata ccggattacg cttcacacgc gatgcgccct ggcccgttat
     8341 ccagtcaccg ttaatccata aagtcatgct gttttctcct ctgcgcacag gcgcaccaga
     8401 cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt gtgcggcggt taaaatcaaa
     8461 cgctcggttg ccggatcggt acgcgccagc accacgcgga aatggtgata attttcattg
     8521 gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc cttctgccac ttccaccagc
     8581 cgacttttac gaatggcgcg cacgcggtcg atgtcacact caagcgttgg cccgccgtcg
     8641 aagatgtcga tatagttgcg gtaacgaaaa ccttctttct ccagcaccgc gcgggcaggc
     8701 gcggtttgcg gatgtacctg accgatgacg tcctgggctt cctgggataa aaagtgggta
     8761 tagatcggat gtttcggcat cagttctgca ataaatgcct tttgcccggt gccgcagaga
     8821 aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac cgaggctttg ccagaacggt
     8881 gaatagccgt gttcgtcaat caccccgcgc atttcagcaa ccactttgtc attaaacttg
     8941 tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca gcaaatagcc gttgccctct
     9001 ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt gtgatcgtta
     9061 ctgagaaaca gcgtcggcaa tgcgttatag acattcagct cttttgaggc gtgaaccaac
     9121 gtgccgacgc gatagttgta ccagggatcg ttcagcccaa ccgccacctc aatggcacaa
     9181 atccccgcca cggtgcctgt ctcgctatct tccagcacga acacatagcc ctgctcactt
     9241 ttgggcagtt cgccttgcca ggttttgatt gccctttcga tacgcgccga aagcgtggct
     9301 tcattggcgg gaagcgacgt caggccgccg cccgttttgc tggcaagctg catcagcgcc
     9361 gagacatctg atcgctcaac gggacggatg accatcatga tgaacctcgg ctaacaaagt
     9421 gttcgcaagc agctgcaaag cgatccagtc cggtcgtcac ctcttcttcg ctgacattga
     9481 gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag taccatcacg cctgctttcg
     9541 ccgcttcctg agagatctgt ttcgcttgcc cggcgtaatc ggcattcagt acacagccaa
     9601 tcagcaaacc taagccgcga acttcactga acaaaccata gcggtgatta atagtattaa
     9661 gacgctcaac aaaccagtcg tgacgctgtt taacgccatt aagcatctct ggtgtgttga
     9721 tgagctccag cactttgcct gccaccgccg aggccagcgg attaccgcca taggtggtgc
     9781 catgagtgcc aacggtcatc acgcgggcgc actcttcggt tgccaacaac gcaccgaccg
     9841 ggaaaccgcc gcccagcgct ttggcggtag ttaacagatc aggcgtcacg ccgtagtgca
     9901 tataggcata cagttccccg gtgcgcccga cgccggtttg tacttcatca aaaatcaaca
     9961 gcgcattgtg gcggttacac aattcacgca gaccttgtaa aaacgcgttg ctggctggca
    10021 ccacaccgcc ttccccctgg atgggttcga caatcaccgc acaggtagag tcgtcaatca
    10081 gcgcgctggc agagttaata tcgttatatg cagcatgacg aatatccgcc ggcagtggcg
    10141 caaaatcctg tgaataggct ggctgcccac ccgcactgac agtaaacagc gtgcgaccat
    10201 gaaacgcatt tttgaacgcc acgatgccgc tcttatggct gccgtagcgg tcgtgagcga
    10261 atttacgcgc cagttttagc gccgcttcgt tggcttccgc accggagtta caaaagaaga
    10321 cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc ggctcgttgg
    10381 tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc gttcagcgct tcacgcagtt
    10441 ccggatgcgc atggcccagc gcgttcaccg caatgccacc cgcgaagtcg atatactctt
    10501 tccccagctg atcccacaag cgcgaacctt cgccacgtac cggtataaag ggtgccggag
    10561 cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt aattggctga gacatagcga
    10621 cctctacagt aaataattcg ttatttatat gttaacaata agtaatgttt gcgctgtaaa
    10681 tgtagattgc agggttcgtg ccagccagtg ataaaagtgc ataaacggcg gaggctaact
    10741 ggaaatcaag gagttataac caaaccatat gcatttaaag tgcatataaa gtgaatacgt
    10801 ttgcgatgtg ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag taaaatataa
    10861 ttctggaatt gtgatcattg acgaaattta ctggaaatta ctgcgccatt ctgacgcagc
    10921 gcgcaccaaa agcgggcatt ttttgcgcca tcgttgacat cattaacaac catcgatcaa
    10981 atcacttaac aacaggcggt aagcaacgcg aaattctgct accatccacg cactctttat
    11041 ctgaataaat ggcagcgact atgaaatttg tctcttttaa tatcaacggc ctgcgcgcca
    11101 gacctcacca gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg
    11161 agacaaaagt tcatgacgat atgtttccgc tcgaagaggt ggcgaagctc ggctacaacg
    11221 tgttttatca cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa gagacgccga
    11281 ttgccgtgcg tcgcggcttt cccggtgacg acgaagaggc gcagcggcgg attattatgg
    11341 cggaaatccc ctcactgctg ggtaatgtca ccgtgatcaa cggttacttc ccgcagggtg
    11401 aaagccgcga ccatccgata aaattcccgg caaaagcgca gttttatcag aatctgcaaa
    11461 actacctgga aaccgaactc aaacgtgata atccggtact gattatgggc gatatgaata
    11521 tcagccctac agatctggat atcggcattg gcgaagaaaa ccgtaagcgc tggctgcgta
    11581 ccggtaaatg ctctttcctg ccggaagagc gcgaatggat ggacaggctg atgagctggg
    11641 ggttggtcga taccttccgc catgctaatc cgcaaacagc agatcgtttc tcatggtttg
    11701 attaccgctc aaaaggtttt gacgataacc gtggtctgcg catcgacctg ctgctcgcca
    11761 gccaaccgct ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatt cgcagcatgg
    11821 aaaaaccgtc cgatcacgcc cccgtctggg cgaccttccg ccgctaattt agcagctctc
    11881 ctggctcaaa ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg
    11941 atttgttgcc tgccgtcctt tgttataccg tcactgcgtt tttagttgtc tgaccacttc
    12001 tctattatca agtttgatat aggaaactcc acgatgaacg ctgagcgtaa ctttctgttt
    12061 gcctgtctta tttttgcgct ggtcatttac gctatccacg ctttcggttt attcgatctg
    12121 ctcaccgatt taccccactt acagacactc attcgccaga gcggattgtt cggttatagc
    12181 ctctatattc tgttattcat cattgccacc ctctttctgt taccaggaag catactggtg
    12241 atcgctggag gaatagtttt tggcccgctc ttagggacac tactctcatt aattgccgca
    12301 acgctggcct cctcgtgctc attcctgctg gcgcgctggc tgggacgtga tttactgctg
    12361 aaatacgtgg gccatagcca taccttccag gccattgaaa aaggcattgc gcgtaacggt
    12421 attgattttc ttattctgac ccgcttaatc ccgttgtttc cttacaatat tcaaaattac
    12481 gcttacggat taaccaccat cgccttctgg ccttataccc ttatttcggc actcacgacc
    12541 ctgctcggta ttgttattta taccgtgatg gcaagcgatc tcgccaatga aggcattacg
    12601 ctgcgcttta ttttacaact ctgtctggcg ggcctggcgc tgtttattct cgtccagctc
    12661 gcaaaactct acgcccgaca caaacatgtg gatctgtctg cttcgcgccg cagcccactt
    12721 actcacccaa aaaatgaagg atagaacgat gtcgcaacat tattcagtgt catggaaaaa
    12781 aggactggct gcactctgct tactggctgt tgccgggctt agcggctgcg atcaaaaaga
    12841 gaatgcggcg gcaaaagtgg aatacgacgg actttcgaac agccaaccgc tgcgcgtcga
    12901 tgccaataac catacggtaa ccatgctggt gcaaattaat ggtcgtttcc tcaccgacga
    12961 cactcgtcac ggtattgtgt ttaaagatgg ctccaacgga cataaatcgc tgtttatggc
    13021 ttatgcgacc ccgaaagcat tttatgaagc cctgaaagag gcaggtggta cgccgggcga
    13081 aaacatgacg atggataata aagaaacgac tcatgtcaca ggcagcaaac tggatatttc
    13141 ggtcaactgg caaggggcgg caaaagcgta ttccttcgat gaagtgattg ttgacagtaa
    13201 tggcaagaaa ctggacatgc gctttggcgg taatttaacg gcagcagaag agaagaaaac
    13261 gggttgcctg gtgtgtctgg atagctgccc ggtcggcatc gtcagcaatg caacatacac
    13321 ttatggtgcg gtggaaaaac gcggcgaagt gaaattcaaa ggcaatgcct cagttctccc
    13381 ggcggataac acgctggcaa cggttacctt taaaatcacc gaataaagcc aggataaagg
    13441 atgatgaaga tacaatcgcg taaaatctgg tactaccgta taaccctcat catcctgttg
    13501 ttcgctatgc tgctcgcatg ggcgctgctt cccggcgtcc atgagtttat caatcgcagc
    13561 gttgcggcgt ttgccgccgt ggaccaacag ggtatagaac gctttattca gtcttacggc
    13621 gcactggcgg cagttgtctc gttcttgttg atgattttac aggccattgc tgcaccgcta
    13681 cctgcgtttt tgatcacctt tgccaatgcg tcgctgtttg gcgcgttctg gggcggcttg
    13741 ctgtcgtgga ccagttcgat ggccggcgcg gcgctgtgct tttttatcgc cagagtgatg
    13801 ggccgcgaag tggtggaaaa attaaccggc aaaaccgtgc ttgacagtat ggacggcttt
    13861 ttcactcgct acggcaaaca caccatcctg gtctgtcggt tattgccttt tgtccctttc
    13921 gatccaatca gctatgctgc cggtctgact tcaatacgtt ttcgctcgtt ttttatcgcc
    13981 accgggcttg gtcaattacc ggcgactatt gtttattcct gggcgggcag catgttaaca
    14041 ggcggtactt tctggtttgt caccggactg tttattctgt ttgccctgac cgtggtgatt
    14101 tttatggcga agaaaatatg gcttgagcgc cagaagagga atgcctgatg ggtttaccgc
    14161 cgcttagcaa aattcctttc attttacgtc cgcaggcgtg gctgcatcgt cgccattacg
    14221 gcgaggtgct aagcccgatt cgctggtggg ggcggatccc gtttatcttt tatttggtgt
    14281 caatgtttgt tgggtggctg gagcgcaaac gctcaccgct cgatccggta gtgcgatcgc
    14341 ttgtcagcgc gcgcattgcg caaatgtgcc tgtgtgagtt ttgcgtggat atcaccagta
    14401 tgaaagtcgc cgagcgtacc ggcagcaccg ataaactgct ggcagtggct gactggcggc
    14461 aaagcccgct cttcagcgat gaagaacggc tggcgctgga gtacgccgaa gccgcaagcg
    14521 taacgccgcc aacggtcgat gatgccctgc gcacccgact ggctgcgcat tttgacgctc
    14581 aggcgctcac cgaactgacg gcattgatcg gcctgcaaaa cctgtcggct cgttttaatt
    14641 ctgccatgga cattcccgct caggggctgt gccgtattcc ggaaaaacgt tcttaaggag
    14701 agaagatgcg ccattgtggg tggttgctgg gattgttatc gctgttttct ctggcaacac
    14761 atgccagtga ctggcaggaa attaaaaatg aggccaaagg gcaaaccgtc tggtttaacg
    14821 cctggggcgg cgataccgca attaaccgct atctcgactg ggttagcggc gagatgaaaa
    14881 cccattacgc tataaacctg aagattgttc gtctggcgga tgccgcagac gcggtgaagc
    14941 gcattcagac cgaagctgct gccggacgta aaacgggcgg ctcggtggat ctgctctggg
    15001 tgaacggcga aaacttccgc accttaaaag aggccaattt attacaaacg ggctgggcgg
    15061 agactctgcc caactggcgc tatgtcgaca cacagctgcc ggtgcgggaa gatttttcag
    15121 tgcctacaga aggggctgaa tcgccctggg gcggcgcaca actgacgttt atcgcccgcc
    15181 gcgatgttac gccacagcca ccacaaacgc cgcaagcctt actggagttt gctaaagcca
    15241 atcccggcac ggttacctat ccgcgcccac cggactttac cggcacggcg tttcttgaac
    15301 agttgctgat tatgctgacg cccgatcccg ccgcgttaaa agttgccccg gataaagcca
    15361 ctttcgcccg cgtcactgcg cccttgtggc aatatcttga cgcgctacat ccctatttat
    15421 ggcgcaaagg aaaggatttc ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg
    15481 gcacattgcg cctgtcgctg acctttaacc ctgctcatgc ccagcaaaaa atcgccagcg
    15541 gcgatttgcc tgcaagcagt tacagttttg gttttaacca ggggatgatt ggcaacgtgc
    15601 atttcgtcac cattcctgct aacgcaaatg ccagtgctgc ggcgaaggtg gttgccaact
    15661 tcctgctctc acccaatgcg caactgcgta aagcggatcc cgctgtctgg ggcgatcctt
    15721 ctgttctcga tccgcaaaaa ctgcctgatg ggcagcgcga aacattgcaa tcaagaatgc
    15781 cgcaagatct gccgccggta ctggctgaac cgcacgcagg ttgggtaaat gcgctggaac
    15841 aagaatggct acaccgttac ggtacgcatt aatctttttg ctgtgggcga tggtggcggt
    15901 gatttatgca ccactgatcc cggcagctct cacgctgata tcgcctgctt tgtcgttgac
    15961 acactggcag gcgttatttg ccgatccaca gttaccgcag gcattactgg caacgctggt
    16021 gtcgacaacc atcgcggcgg tcggggcgtt gttgattgcc ctgctggtga ttgtggcgct
    16081 gtggccttgg ccgaaatggc agcgtatgtg tgcccgtctg ccgtggctgc tcgccattcc
    16141 ccatgtggct tttgccacca gcgcccttct gctctttgct gacggagggc tgctttatga
    16201 ctatttcccg tatttcactc cgctaatgga caaattgggt attgggctgg gcctcaccct
    16261 tgcagtgaaa gaaagcgcat ttctgctgtg gatcttagcg gcagtattga gcgaaaaacg
    16321 gctgttgcag caggtcattg tgctggattc gctgggctac agccgctggc aatgcctgaa
    16381 ctggctgctc ttgccctccg tcgcacctgc actggcaatg gcgatgttgg cgattgttgc
    16441 ctggtcgctt tcggtcgtgg atgtggcgat tattcttggg ccaggtaatc caccgacgct
    16501 ggcagtaatt agctggcagt ggttaactca gggcgacgcg gatcaacaaa cgaaaggcgc
    16561 actcgccagt ctgctgctga tgctgttact cgccgcctac gttttgctga gctatctgct
    16621 atggcgcagc tggcggcgca ctattccccg cgtagatggc attcgcaagc ctgccacacc
    16681 tttattacca ggcactacgc tggcgagttt tttaccctta accggtgtgc tgtgtgtggt
    16741 tctgctggcg atcctcgcgg atcagtcgac gatcaatagt gaagcactca tcaacagcct
    16801 gacaatgggg ctggtggcga cattcatcgc tttgctcctg ttactgctgt ggctggaatg
    16861 ggggccacag cgtcgccagt tgtggctctg gttgcccact ctattgcccg ctctgccact
    16921 ggtggcgggc cagtacacgc tggcgctatg gctgaacctg gatggaagct ggacggcggt
    16981 ggtctggggg catctgctgt gggtaatgcc gtggatgctg tttatcctgc aacccgcctg
    17041 gcagcgcatt gattcacggt taattttgat tgcgcaaaca ctgagctggt cgcgggccaa
    17101 aatcttcttt tacgtgaaat gtccgcttat gctacgccct acgctgattg ccttcgcggt
    17161 gggattttca gtcagtattg cgcagtatat gccgacgctg tggctgggcg cggggcgttt
    17221 tccgacgctc accactgagg cggtggcatt aagcagcggc ggcagcaatg gtattttcgc
    17281 cgcccaggct ttatggcaac tgctattacc gcttattatt tttgccctga ccgcgttagt
    17341 cgcaaaatgg gtaggttatg tcagacaagg actccgctaa tgctctgcgt gaaaaatgtt
    17401 tcgctacgtt taccagaaag ccgcttgctg acaaacgtta actttacggt ggataaaggt
    17461 gacattgtca cgttaatggg gccgtctggc tgtggaaaat ccactctgtt ttcatggatg
    17521 attggtgcac tggctggaca gttttcttgt acaggtgagc tatggctcaa tgagcaacgg
    17581 attgacatgc tacccaccgc acagcgtcag attggcattc ttttccagga tgcactgtta
    17641 tttgaccagt tcagcgtcgg gcaaaattta ctgctggcgc taccgtcgac gctcaagggg
    17701 actgccaggc gcaatgccgt gaaagacgcg cttgatcgag cgggactggc tgaaacttat
    17761 caccaggacc ctgcgacatt atcaggcggt cagcgagcgc gggtggccct gctgcgcgcc
    17821 cttctcgctc agccaaaagc gttactcctg gatgagccat tcagccgtct tgatgtggct
    17881 ctgcgcgata attttcgcca gtgggtgttc agcgaagttc gcgaactggc gatccccgtc
    17941 gttcaggtaa cgcacgatct ccaggatgtt cctgctgata gttctgttct ggatatggag
    18001 cagtggtcag aaaattacaa caaactgcga taacgcaaag tttttctcaa tgcgtcagtt
    18061 cagaatggcg cactcaaaac tacaatgtcg ggattttcga tgaaacgtgt ttctcaaatg
    18121 accgcgctgg caatggcttt agggctggct tgcgcttctt cgtgggccgc tgaactggcg
    18181 aagcctctta cacttgacca gcttcaacaa caaaatggca aagcgataga tactcgcccc
    18241 agcgcgtttt ataacggctg gccacaaacc ttaaatggcc cttctggtca tgaacctgcc
    18301 gccttaaacc tctctgccag ctggctcgac aaaatgagca ccgaacagct caacgcgtgg
    18361 atcaagcaac ataacctgaa agccgatgcc ccggtggcgc tgtacggtaa tgacaaagat
    18421 gtcgacgccg tcaaaacgcg actgcaaaaa gcaggtttta cgcatatctc cattctgagt
    18481 gacgcgctaa gcgaaccttc ccgtctacaa aaactgccgc actttgagca gctggtttat
    18541 ccgcaatggc tgcacgacct gcaacaaggt aaagaggtta cggcgaaacc tgccggtgac
    18601 tggaaagtca ttgaagcggc ctggggcgct cctaagcttt accttatcag ccatattccc
    18661 ggtgctgact acatcgatac caacgaagtg gaaagcgaac cgctgtggaa caaagtttct
    18721 gatgaacaac taaaagcgat gctggcaaaa cacggcattc gccatgacac cacggtcatt
    18781 ctgtacgggc gtgacgtata cgctggagcg cgtgtggcgc aaattatgct ctacgcaggc
    18841 gtgaaagatg tgcgtctgct ggatggcggc tggcaaacct ggtccgacgc gggactgcct
    18901 gttgagcgag gaacgccacc gaaagtgaaa gcggaaccgg atttcggcgt gaagatcccg
    18961 gcacaaccgc agttgatgct cgatatggaa caggcccgtg gactgctgca tcgccaggat
    19021 gcatcgctgg tgagcattcg ttcgtggcca gaatttatcg gtacgaccag cggttacagc
    19081 tatattaaac caaaaggtga aatagccgga gcacgttggg gacacgctgg tagcgactcg
    19141 acgcatatgg aagattttca taacccggat ggcaccatgc gcagcgccga tgatattacc
    19201 gctatgtgga aagcgtggaa tatcaaacca gagcagcaag tttcattcta ctgcggcacc
    19261 ggctggcgcg cgtccgaaac ctttatgtac gcacgcgcca tgggttggaa gaatgtttcc
    19321 gtgtatgacg gcggctggta cgaatggagc agcgatccaa aaaatccggt agcaaccggt
    19381 gaacgcggcc cggacagtag caaataacat tgcaattact gacgctggtg cgacttcagc
    19441 gtcagatacc cactccacac ccgcgtaaag gtcgtcatcc agcacaacgc accgaagatc
    19501 cacgcaaacc acgcaaacca tgcagggaat aaacatccca gcacaaacag taagatcgtt
    19561 tctgtgcctt cggttaaccc gcccagataa taaaacgatt tatgcgcata accgggatta
    19621 tcaatctgat gtttcgccgc cagcgcagca aaagcgagaa aactgctgcc tgtgccgata
    19681 aacgcaaaca gcagccagcc gcctgccagg gcgttttgct gcggtgcggc gaggataaac
    19741 ccaaaaggta ccagcgcgta aaaaagaaaa tcgagagaaa tatcaagaaa gccgcccgcg
    19801 tcggtaagcc ctctgcgccg cgccagcgca ccgtccagac catcaagcag cctgttcaac
    19861 aaaatgacga caagcgccgc cagataccag cccagcgcca gaaaaggcag tgccagcacg
    19921 ccgatggcaa atcccactaa tgttaaacca tctggcgtaa tgcccggttt atcaagaacc
    19981 cgcacgcact gatgcaacaa cggtttaatc cggggatgaa gatggcggtc tagcacaggc
    20041 actccttaaa tataaagcct ttctgattga gcaacagtgc ggatattatg gcatttttcg
    20101 cttatctgcc cgtgtgtaat ttatgaaaat gattgaagtt gttgccgcca tcattgaacg
    20161 tgatggcaaa attttactcg cgcaacgccc cgcccagagc gatcaggcgg gattatggga
    20221 gtttgccggt ggtaaagtcg agccggatga aagccagcgg caggcgctgg tgcgtgagtt
    20281 acgcgaagaa ctgggcatcg aagcaactgt gggtgagtat gttaccagcc atcagcgaga
    20341 agtttcgggc cggattatcc atcttcatgc ctggcacgta cccgacttcc acgggacgtt
    20401 acaggcacat gaacatcagg cgctggtctg gtgctcacct gaagaggcgc tgcaatatcc
    20461 gctggcccct gctgacattc cattattaga ggcgtttatg gctttacgcg ccgccagacc
    20521 agcggattag tgctaagggt tttgtcatcc cgctggcatt gcagcagtat tccctcggct
    20581 ttaattaccg cgccttcaga ataattttta tcctgataaa cgcagcactg agtacagggc
    20641 tgcgctgact gcccgcctga actgaacact tctggcggta cgtttacctc cacgtccgga
    20701 cgataatgcg ggttagccag tgcaattaat ggaaatgcta atactacggc gaacaatgct
    20761 cgactcacag ggaactcctt aacgttattg tctctgctac tgataacggt agccgggtgg
    20821 caaaacttta gcgtctgagt tatcgcattt ggttatgaga ttactctcgt tattaatttg
    20881 ctttcctggg tcattttttt cttgcttacc gtcacattct tgatggtata gtcgaaaact
    20941 gcaaaagcac atcacataaa caacataagc acaatcgtat taatatataa gggttttata
    21001 tctatggatc agacatattc tctggagtca ttcctcaacc atgtccaaaa gcgcgacccg
    21061 aatcaaaccg agttcgcgca agccgttcgt gaagtaatga ccacactctg gccttttctt
    21121 gaacaaaatc caaaatatcg ccagatgtca ttactggagc gtctggttga accggagcgc
    21181 gtgatccagt ttcgcgtggt atgggttgat gatcgcaatc aggtacaggt caaccgtgca
    21241 tggcgtgtgc agttcagctc tgccatcggc ccgtacaaag gcggtatgcg cttccatccg
    21301 tcagttaacc tctccattct taaattcctc ggcttcgaac aaaccttcaa aaatgccctg
    21361 actaccctgc caatgggcgg tggtaaaggc ggcagcgatt tcgatccgaa aggaaaaagc
    21421 gaaggtgaag tgatgcgttt ttgccaggcg ctgatgactg aactgtatcg ccacctgggc
    21481 gcggataccg acgttccggc aggtgatatc ggggttggtg gtcgtgaagt cggctttatg
    21541 gcggggatga tgaaaaagct ctccaacaat accgcctgcg tcttcaccgg taagggcctt
    21601 tcatttggcg gcagtcttat tcgcccggaa gctaccggct acggtctggt ttatttcaca
    21661 gaagcaatgc taaaacgcca cggtatgggt tttgaaggga tgcgcgtttc cgtttctggc
    21721 tccggcaacg tcgcccagta cgctatcgaa aaagcgatgg aatttggtgc tcgtgtgatc
    21781 actgcgtcag actccagcgg cactgtagtt gatgaaagcg gattcacgaa agagaaactg
    21841 gcacgtctta tcgaaatcaa agccagccgc gatggtcgag tggcagatta tgccaaagaa
    21901 tttggtctgg tctatctcga acgccaacag ccgtggtctg taccggttga tatcgccctg
    21961 ccttgcgcca cccagaacga actggatgtt gacgccgcgc atcagcttat cgccaatggc
    22021 gttaaagccg tcgccgaagg ggcaaatatg ccgaccacca tcgaagcgac tgaactgttc
    22081 cagcaggcag gcgtactgtt tgcaccgggt aaagcggcta atgctggcgg tgtagcaacg
    22141 tcaggccttg aaatggcgca aaacgctgcg cgcctgggct ggaaagccga gaaagttgac
    22201 gcacgtttgc atcacatcat gctggatatc caccatgcct gtgttgagca tggtggtgaa
    22261 ggtgagcaaa ccaactacgt gcagggcgcg aacattgccg gttttgtgaa ggttgccgat
    22321 gcgatgctgg cgcagggtgt gatttaagtt gtaaatgcct gatggcgcta cgcttatcag
    22381 gcctacaaat gggcacaatt cattgcagtt acgctctaat gtaggccggg caagcgcagc
    22441 gcccccggca aaatttcagg cgtttatgag tatttaacgg atgatgctcc ccacggaaca
    22501 tttcttatgg gccaacggca tttcttactg tagtgctccc aaaactgctt gtcgtaacga
    22561 taacacgctt caagttcagc atccgttaac tttctgcgat agcagcagat atgccagtaa
    22621 agaaacccca ttagactatt tttttgataa tcttcttcgc tttcgaacaa ctcgtgcgcc
    22681 tttcgagaag caagcattat ataatgccag gccagttctt cttcaattgt cccgttttga
    22741 aaagctgtgc ttgatatcga ggtcatccat gataattccg ccgcccatat tagcttcgcc
    22801 gaggatttac cggagcgatt attagcgcaa tcagagatat agtctgaggg aaaaacagca
    22861 aatttattca acaaggcgat aacctgctct ggggcttcct ccatgtttgc tttaaaggta
    22921 ttggctccat ggtcgccaga aagaaaatac tccattaagg cacaataact ttcgctatct
    22981 tcgatacccc attgatcctc taaagactcg cgtcttttgc ttatgatatc gatcgagtca
    23041 aagggaagca catgatattg gaaggtatct ttgccaggtt caggctttcg cggccagaac
    23101 tccagcgttt cagaccattg cttatgatag aaccgataag gtgcgatcaa ttgtagcgcc
    23161 tgtaacttct cgatactgag cggctcaata cctttagcct gataataatg cagttgttct
    23221 ttttttgctt taaaaccggc ccgaacaata agccccatca taattaatag ataaagaaaa
    23281 gagcatcccg cggtaatcag gcctctttca ttcaaaccgt tggatgttat cgctgcgaac
    23341 acaaacatta cagcgacaac acatgttaaa taaaaccccc acttacaaag cagcatggcc
    23401 ttattttctt taatcatccg ttcaaaatta ctattaaata tttcccagcc attaaaagaa
    23461 tacttctcgc tcccaggatg gttttgtaat aaaacttttt tcatcacatt ccctgttatt
    23521 acatacttta gaggcgataa ttttatccat gcaaaaaaaa tatccaacga attttcttga
    23581 tccgctggat atttcagaat atgactcgaa tagcacgaaa gattcactcg cttacgctat
    23641 cgccccgctt ccgacttcat ctgctggcgg actttttttc gcactacgtt tacgcggtgc
    23701 agcctttttc ttatcagcac tgccaccact gcccggagcc acaatgccgc gaaactgccg
    23761 caccggcgta cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat
    23821 aaagtcctga tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc
    23881 ggtcatgtcc ggtcgcgtcg ccatttctgg cagcgaatgg aataacgctt ttccggcgtc
    23941 ggtagagtgg atatagcgcc cttttttggt cagaaaacca cgcttgaaca gcagttcaat
    24001 aatcccggca cgggttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag
    24061 atctttgtcc tgcacaaagc gcgcgatccc ggtcatcgcc gaaagcagtg ttgcatcggt
    24121 aaaatggcgc ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc
    24181 atcgcctttc gccaccacag gcaatggcat accgtcgttt tcttcatcgc gctctttgct
    24241 gcctaacagc gtgcgccagc ctgcttcagc aagaaaacgg gctttagcga caaatttgcc
    24301 tttggcaatg tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat
    24361 caggtattga cgggcaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc
    24421 agaactccgt gcggtcggga tgatggcgtg gtgcgcatcg acctttttgt catcccaaca
    24481 gcggttgcgt atatctggat ctaccactga ctgcggcaac agatccggtg catgaacgct
    24541 gatggcattc atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc
    24601 ggaacgcgga taagtgatta gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac
    24661 gttctgcgcg ctcagaccga agcgttttgc cgcttcaatc tgtaacgccg aaagcgaaaa
    24721 cggcagcggc gcggattctg attcccgttt atcgttatag ctggtgacaa tagccggttg
    24781 accactaatg cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc
    24841 atcctggtac ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc
    24901 aggtgtcacg atatgcgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc
    24961 ttcatcgcgg cgcaccacca gcccgagcac gggtgtctgc acgcgtccca cggaaagtac
    25021 gccctgataa ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta
    25081 cagccagtcg gcacgcgctc gcgccagcgc agaaacgcac agcggtacaa actcactgtt
    25141 ggaacgaaga cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca
    25201 acgctgcacc tgctggcgct tttccggtgc cagttgcaga tagtccagca cttcatccac
    25261 cagcaattgc ccttcacgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag
    25321 gaagcgtttg atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt
    25381 ttccgggaca atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc
    25441 tggctgcgcc tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca
    25501 ctcgataaag ccatcgcctt tacggtgcgg tttgggcagg acatcagcaa tggcgcgcgc
    25561 cagactcggt ttttcggcaa taaacaaccg cattgaatta atggatctca accatggcac
    25621 gaccgccgcg cgctggcacc agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg
    25681 ctgtagtttt aacctcattt tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag
    25741 tttgcggatc gcacagcaga tcgcgcactt cacgcggcat ttcacccatc agatgaccgt
    25801 agctggcaaa gttacgttcg gtgccgccag gcactgcgcc caacttaatg tactcttcaa
    25861 cccccggcaa ttttgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac
    25921 acatttcgct caaatggccc agcagaccaa aaccagtaac gtcggtcatc gcttttacgc
    25981 cttcgatgtt ggcaaaggac gcacctgcga tgttcatccg gcacatcact tccgtcgcca
    26041 gtccctgatg ttctggtttc aacagtgatt ttttctcagc cgtggtaaga acgccgatcc
    26101 ccagcggttt cgtcaggaac agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc
    26161 gctcggtcgg tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg
    26221 agtgaccgcc agccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta
    26281 cttcgcgggc aatttctggg gaaagtttgt taatcggcca gccgaggatc gcaatcgcca
    26341 taatcggttt gccccccatc gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc
    26401 caaaatcgaa aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg
    26461 tgccattgcc cagatcgtac accgccgcat cgtcgcgggt ctcattaccc acaagcaaat
    26521 tcggatcaac aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa
    26581 ttttacagcc gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca
    26641 tggacatctc ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt
    26701 gagagtgtct gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca
    26761 gaaataagtt acaaacggcg tcgggtccgg gacgttaatc gacgtagatg ctttcaactg
    26821 cggcgtgccg aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc
    26881 acgcactacc ggactttcag ttaatgcgcc actgcgccag atgccgccaa atccttgggc
    26941 aacggctgcc atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg
    27001 cgggacttta tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa
    27061 cggcgcatta cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg
    27121 ttccagtacg gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg
    27181 ttgcatggac ttatggtccg gcgcacgcat acccgcacgc aggatgtttt gcagttgttc
    27241 acccgttggc gcgggttcag ccaggcggga ggcgctacgg cgattgatca atagttcgag
    27301 tgcatccatt tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga
    27361 ttttgttaca gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa
    27421 ccacatctat tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct
    27481 ttggcgattt attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga
    27541 aatggtactt aacctgttct ttattttcct cgtactggtt ggtgtgggga tctggatgca
    27601 ggtcagtggt ggtgattcga aagaaacggc cagtcgtggc gcactgctgc tggacatttc
    27661 tggtgtgatc gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccagct
    27721 gcttggtgcc agttccgatc gtctgcagga aaactcactg tttgatatcg tcaacaccat
    27781 tcgccaggcg aaggacgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc
    27841 aggcggcgac cagccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag
    27901 cgggaaaccg gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag
    27961 tttcgccaat aaaatttggc tgtcaccgca aggcgtggtt gatctgcacg gttttgctac
    28021 caacggtctg tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg
    28081 cgtgggtacg tataaatctg ccgttgaacc gtttattcgt gatgatatgt caccggcagc
    28141 ccgcgaagct gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc
    28201 cgctaaccgg cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg
    28261 tttaaccaaa accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc
    28321 actggcatcg agtgccgaaa tcgaaaaagc tctgaccaaa gagttcggct ggagtaagac
    28381 tgataaaaat tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac
    28441 cggtgacagc atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca
    28501 ggggaatgtt ggcggtggta ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa
    28561 agtgaaagcg attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt
    28621 gattcgcgct gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg
    28681 cggcatggcg gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa
    28741 ccccagcacc ctgaccggtt ctatcggtat cttcggcgtg atcaccaccg tagaaaatag
    28801 tctggattcg attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc
    28861 tatcaccagg gcactgccgc cggaagcgca gcagatgatg caattaagca ttgagaatgg
    28921 ctataaacgc tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcaaattga
    28981 taaaatcgcc cagggccacg tctggaccgg tcaggatgca aaagctaacg ggctggtcga
    29041 tagtctcggg gatttcgatg atgcggttgc caaagcagca gagctggcaa aagtgaaaca
    29101 gtggcatctg gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat
    29161 gtctggttct gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct
    29221 tgcctcggta gcctctaccg ttaaaagtga aagcgacaag ctggccgcgt ttaacgaccc
    29281 acaaaaccgt tatgcgtttt gcctgacctg cgccaacgtg cgttaagtct tgtactgagt
    29341 ggccgacaga tcgtcggcca cattattttt tacctcgacg aatcctcttc ccgctgtctc
    29401 gacccatatt tccttatccg catagtatca ggtgcgctcc tcctgcctca cgtatatact
    29461 tttgctcttt cgatatcatt catatcaata tcatgcaaaa gaaatcaatt tacgtcgcct
    29521 acacgggcgg gaccatcggg atgcagcgtt ccgagcaggg ttatattccg gtgtcaggtc
    29581 atctacaacg ccaactggcg ctgatgccgg aattccatcg cccggagatg ccagatttca
    29641 ccattcatga atatacgccg ttgatggatt cttctgatat gacgccggaa gactggcagc
    29701 atattgctga agatattaaa gcgcactatg acgactatga tggttttgtc attctacacg
    29761 gtaccgacac gatggcgtat accgcctctg cgctgtcgtt catgctcgag aatctcggta
    29821 aaccggtcat tgtgacaggg tcacaaatcc cgctggctga gttacgctct gacggacaaa
    29881 ttaatctgct gaatgcgttg tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct
    29941 ttttcaataa ccgattgtat cgcggcaacc gcactaccaa agcccatgcc gatggttttg
    30001 atgcgtttgc ctctccaaac cttcctccgt tactggaagc tggtatccat attcgtcgtt
    30061 tgaatacgcc acccgccccg cacggtgaag gggaattaat cgttcatcca atcacgccac
    30121 aaccaattgg cgtagtgacg atttatccag ggatttctgc tgacgtcgtg cgcaattttc
    30181 tgcgccaacc ggtgaaagca ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa
    30241 acaaagcctt cctgcaggaa ttacaagaag ccagcgatcg cggtattgtg gtggtcaacc
    30301 tgacacaatg tatgtccggt aaagtgaata tgggtggtta tgccaccggt aacgccctcg
    30361 cccatgccgg cgttattggc ggtgcagata tgactgtaga agccacgcta accaaactgc
    30421 attacctgct gagccaggaa ctggatactg aaaccattcg caaggccatg agccaaaacc
    30481 tgcgcggcga actgacgccg gatgattaag gagactgcaa tgccccatcg cgccctgtta
    30541 ctggtcgatt tacaaaatga tttctgtgct ggtggcgcgc tcgccgtgcc ggaaggtgac
    30601 agtacggtag atgtcgctaa ccgcctgatt gactggtgcc agtcgcgcgg tgaagcggtt
    30661 atcgccagtc aggactggca cccggcgaat cacggcagtt ttgccagtca gcacggtgta
    30721 gagccttata cgccaggcca actcgacggt ttgccacaaa ccttctggcc agatcactgt
    30781 gtgcagaaca gtgaaggcgc acaattacat ccgttactga accaaaaagc gatcgcagcg
    30841 gtgttccata aaggcgaaaa tcctttagtt gacagttaca gtgccttttt tgataacggc
    30901 cgtcggcaga aaacctctct cgatgactgg ttacgcgatc atgaaatcga tgaattgatc
    30961 gttatgggcc tggctactga ctattgcgtg aagtttaccg tgctggacgc gttacagtta
    31021 ggttataagg taaacgtgat taccgatggt tgtcgtggcg tgaatatcca gccccaggac
    31081 agtgcgcacg cgtttatgga gatgtcagca gctggggcaa cgctatatac gctggcagac
    31141 tgggaagaga cgcaggggta atattagttg aattcatctc tggcctacat ttcctcaatg
    31201 acattgtagg ccagataata cttgtcagtg ttgcatttaa cctacttgca gctaaaacac
    31261 gacgtcctaa caagtatatt tgccctccct taataacagg cttaaatact agataactca
    31321 ttcacttaaa catagcaata ggctttgctg agataccaaa atcttctttt aactgctgtt
    31381 tgctcttcat gaccatctga ccatttgtgt caattgtcat atgctgtgct tcggtattat
    31441 ggcgggcttg ccacagcatc accagttgca ggcaattctc tttttgttcc tcggtcaacg
    31501 caacgccatc aggccatttc cccagttcaa cggcggtcga caaacgctgg tatacctcag
    31561 gcgtcatgct gttgataatg tcatcaagat tcataatttt tccgctcctg tggaataagt
    31621 tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt tcgccatcag tgaagcgtaa
    31681 agaagcagag ttaacacaat aacgttcgcc tgttggttgc ggcccgtcgg gaaagacatg
    31741 ccccagatgg gcatcacagt taccgcaacg aatttctatg cgctgcattc catgtgacaa
    31801 gtctttgata taacgaatgg attcttcact caccggttcg tagaaactgg gccagccaca
    31861 gccggaatca tacttggttt cggaatgaaa cagcggggca tcgcagatca aacagtgata
    31921 tacgccgtca cgcttgttat gcagtaaacg acccgtaaat ggcggttctg tcccatgatt
    31981 ctgcgtcacg taaaactgca tctcggacaa attttttttc agttcttctg ccgaaggttt
    32041 attagccatt tgctcacatc tcactttaat cgtgctcaca ttacgtgact gattctaaca
    32101 aaacattaac accaactggc aaaattttgt cctaaacttg atctcgacga aatggctgca
    32161 cctaaatcgt gatgaaaatc acatttttat cgtaattgcc ctttaaaatt cggggcgccg
    32221 accccatgtg gtctcaagcc caaaggaaga gtgaggcgag tcagtcgcgt aatgcttagg
    32281 cacaggattg atttgtcgca atgattgaca cgattccgct tgacgctgcg taaggttttt
    32341 gtaattttac aggcaacctt ttattcacta acaaatagct ggtggaatat atgactatca
    32401 aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct gctcagaaac
    32461 gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac atggcataca
    32521 tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg aaagacggtc
    32581 atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg gctaacctga
    32641 aatgggacga agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc ctgactgacg
    32701 aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact ggtccgtcta
    32761 aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct ggccaggaca
    32821 tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa gttatcaacg
    32881 ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc gctactcaga
    32941 aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct tcccagaaca
    33001 tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca gaactgaatg
    33061 gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta gttgacctga
    33121 ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt aaagctgctg
    33181 ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta tctaccgatt
    33241 tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct ctgaacgaca
    33301 acttcgtgaa actggtatct tggtacgaca acgaaaccgg ttactccaac aaagttctgg
    33361 acctgatcgc tcacatctcc aaataagttg agatgacact gtgatctaaa aagagcgact
    33421 tcggtcgctc ttttttttac ctgataaaat gaagttaaag gactgcgtca tgattaagaa
    33481 aatttttgcc cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc gtaaactgga
    33541 tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg cattacaggg
    33601 cgcacacctt ctctcgtgga aacctgcggg tgaagaagaa gttctgtggt tgagcaacaa
    33661 cacaccgttc aaaaatggcg tcgctattcg cggtggcgta ccggtttgct ggccgtggtt
    33721 tggtccggcg gcacaacaag gtctgcctgc gcacggtttt gcccgcaacc tgccgtggac
    33781 gctgaaatca catcgtgaag atgctgatgg cgtagcgctg acttttgaat tgacgcaaag
    33841 cgaagagacg aaaaaattct ggccgcacga ctttacgctg ttagcgcatt tccgcgtggg
    33901 taaaacctgt gaaatcgatc ttgaatcaca tggcgaattt gaaaccacct ctgccctgca
    33961 tacctacttt aacgtgggtg atatcgctaa ggtaagcgtc agtgggctgg gcgatcgctt
    34021 cattgataaa gtaaatgacg cgaaagaaga tgtactgacc gacggtattc agaccttccc
    34081 tgaccgtacc gatcgcgtgt atctgaatcc gcaagattgc agcgtgatta atgatgaagc
    34141 gctgaatcgt attatcgccg taggccacca gcatcatctg aacgttgtcg gctggaaccc
    34201 gggaccggcg ctttcagtta gcatgggcga catgccggat gatggctaca aaacatttgt
    34261 ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtg accaaagaga aacctgcaca
    34321 tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg ttaatgttgt gtgccgggtg
    34381 cgatgcatcc ggcacgtatc aacatcacac catatccagc gcagtttttc cttttggtgc
    34441 cggatacgct ttatccagca tggttaattc cgctgacgaa agttcgacct caagcacagc
    34501 cgcattctgt tggacatggg caatcgtggc cgcttttgga atcgccatca caccctgatg
    34561 gctgattacc cacgccaaca atacttgtgc cgcgctgata ttgtgagcat gtgcaatttc
    34621 gttgactacc gcgtttttta acagtccatt gcgcaaccgc ccggcctggg ctaatggact
    34681 gtaagccatc accggcatct gctgttgctg gcaccagggg agtagatcgt actcaatccc
    34741 tcgtgaaccg agatggtaaa gcacctgatt agtggcacac tgatttcccc ccggcagctg
    34801 ccagagttcc tgcatatcag catgatcaag gttagaaacg ccccagcggc ggatttttcc
    34861 ctgggcgatc aatttttcca tcgctgccac agtctcttca aaagcgaaac tgccagacca
    34921 gtgtaataag tagagatcga gataatcagt attgagacgg cgtaaactgg cttcgcatgc
    34981 atttatcgct ttttgcccgc cagcattcca cggatagact ttagagacga gaaagacgtt
    35041 atcgcgcaga ccggttaatg cttccccaac caccttttcg gcaccgccat cggcatacat
    35101 ttcggcggta tcaatgaggg ttaaaccgag ctcaatgccc gcgcgtagtg cagcaacttc
    35161 tgttttgcgc tgactggcat cttcgcccat ataccatgtt ccctgcccta tggctggcag
    35221 tgagacatcg ccactaaatt gaatcatttt ttgttgcatt gtttcctccc aggtattgca
    35281 ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag
    35341 atgcattatc agaatttgta ggtgatcccg gtagaaatca ggccagtcca ggatttatcc
    35401 accatcgggc tgtcagtaac ttcatcagac agacgggtat agcgcgcggt accgtaaaca
    35461 ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca ggtaagggct ccagctgtcg
    35521 ttcgggttat agccacgcag accgctgcga gcggactctt tgcgcgatac gccataatag
    35581 tattcgttct ggttttcgct gttccactgc acaccgatac ccggggtcac ggtcaggccg
    35641 ccgttggtgt aacgatacaa ccaggccata tcccagacga tgccgttgct gttatccagg
    35701 gtatcgccag ccagggtggt acgcaggtaa ccgtactggg taaagtgagc ataagacaga
    35761 ccagccatca tggtgctctt acggtcatcc aggtgacgca tttggtgatc gccactgtct
    35821 ttcgctttga agtaaagcgg cgaccaataa gcggtaattg aaagtttgtc cgttgcgtca
    35881 ttccacaggt agtaaccacc acctaagcca cggaaccaga agttatcgcc ttcatagttg
    35941 attaccggta ctgggtaaac atcggtatcg taatctttat atgggtgctc aacgacacct
    36001 acgcctgcgc ccagggaaaa tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata
    36061 agcactccaa gtgccagaag tttgagtttg gtcacaatta atcattcctt aaacaaatgt
    36121 ttagcgggcg acaaagttta cccgtcaata catcgaaacc caaccttttt acgttttcat
    36181 tttttaaagt aactgtttaa ttttcctgac gcggatgaca ccgcgcttac agccaaatga
    36241 atttagcgtt actggctacc ctggtcttta cattaattat gcaaaattta tggatgagtt
    36301 gttgatatgc cattgaaatt aagaaagccg tgcaggcaag ttttccattt gccatctacg
    36361 cttaattttg aaggtgtatc accgggcacg ttgttctcat cgtcgataaa atggcatgag
    36421 agttgctgtg ttttagcaag agacgtcgtt cagtttacct cttccgggag cctctactat
    36481 tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc atgaatatat
    36541 tcgatcacta tcgccagcga tatgaagctg ccaaggacga agagttcaca ctgcaggagt
    36601 ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag cggctattga
    36661 tggctatcgg tgagcctgtc atggtcgata cagcccagga acccagactt tctcgactct
    36721 tttctaaccg ggtcattgca cgttatccgg catttgaaga gttttacggc atggaagacg
    36781 cgattgaaca gattgtctct tatctgaaac acgcggctca ggggctggaa gagaagaaac
    36841 aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct gagcgactga
    36901 aatcattaat gcagctcgta ccgatttatg tattgagcgc caacggtgag cgtagcccgg
    36961 tcaacgatca tccgttctgt cttttcaatc cgcaggaaga tgctcagatt ctggaaaaag
    37021 agtatggcat tcctcgccgt tatctcggca ccatcatgtc gccatgggcg gcaaaacgcc
    37081 tgcatgaatt tggtggcgat atcactaagt tccgggtagt gaaggtctgg ccgtcaattc
    37141 tgcaacaaat tgctatcgcc aaaacggaac ccggtgatga gaacaaccag gacatctccg
    37201 cgctggttgg gaaagtcgat attcgtaaac tcgaacacta cgcgcagaat gacccggacg
    37261 cctacggcta ttccggtgcg ctgtgccgcg ccaaccaagg gatcatggaa tttgtcgaga
    37321 tgtttaaagc accgataaaa gtgctgcatc cgctgttaac cgccactcag gaaggtaact
    37381 acaacgggac ggaaggtatc tccgccctgc cgttcaacgg gattattctc gcccactcga
    37441 acgaatccga atgggtcact ttccgtaata acaaaaacaa cgaagccttc ctcgaccgtg
    37501 tttacatcgt gaaggtgccg tattgcttac gcatttccga agagatcaaa atctacgaga
    37561 aattgcttaa tcacagtgaa ttgactcacg ccccctgcgc ccctggcacg ctggaaacgc
    37621 tgtcacgttt ttccattctt tcgcgcctta aagagccaga aaactccagc atttattcaa
    37681 agatgcgggt ttatgatggc gaaagtctga aagacaccga tcccaaagcc aagtcgtatc
    37741 aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg
    37801 cgtttaagat cctctcccgc gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg
    37861 tccatctgtt ctacgtcctg gaacagcaga tcgagcgcga gcagttccca caagagcagg
    37921 cagaacgcta tctggagttc ctgaaaggtt atctgatccc gaaatatgcc gagtttatcg
    37981 gcaaagagat ccagacggcc taccttgaat cctattccga atatgggcaa aacattttcg
    38041 accgttatgt tacctacgcg gatttctgga ttcaggatca ggagtatcgc gatccggata
    38101 ccgggcagct gtttgaccgc gagtctctta acgccgagct ggagaaaatc gagaaaccgg
    38161 cggggatcag taatccaaaa gatttccgca acgagattgt taacttcgta ctgcgcgcca
    38221 gagcgaataa cagcggacgc aatccgaact ggaccagcta tgaaaaactg cgcacggtta
    38281 tcgagaagaa aatgttctcc aataccgagg agctgttgcc ggttatctcg tttaacgcca
    38341 aaacgtcaac cgacgagcag aagaaacacg acgactttgt cgaccgtatg atggaaaaag
    38401 gctacacccg taaacaggtg cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt
    38461 cttaacaacc ctggcccggt acacatgtta ccgggcctac aacgacagcg aaccgtgggc
    38521 ctgagaagcg gcaacacagg cgtagcatac agttggcaaa tgtagtacgg ggggcatatg
    38581 acctggttta ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt
    38641 tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat taataagcgt
    38701 tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga tattagcgaa
    38761 ccgatgtttc atcaggggcg tggcggtctg cgccaccgcg tgcatccggg caatgaccat
    38821 ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg gcggaggttc cggtagtggt
    38881 cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca gatttcgaaa
    38941 gatgagtatc ttgatttgct ctttgaagat ttggccttac cgaatctgaa acaaaaccaa
    39001 caacgccagc tgaccgaata taaaacgcat cgggcgggtt ataccgctaa cggcgttccg
    39061 gcaaatatca gcgttgtgcg ctccttgcag aactcactgg cgcgacgcac agccatgacg
    39121 gcaggcaagc ggcgggaact tcatgcactg gaagagaatt tggccatcat cagcaacagt
    39181 gaacctgcgc agctgctgga agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc
    39241 aaaattgaac gcgtcccttt tattgacacc ttcgatttac gttacaagaa ctacgagaag
    39301 cggcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc cggttcaatg
    39361 gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct gttcctcagc
    39421 agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaggc gaaagaagtc
    39481 gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccattgtttc cagcgccctg
    39541 aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa tatttacgct
    39601 gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca tgaaatcctg
    39661 gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac ccgtcgtgca
    39721 catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg
    39781 cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt tcataaacaa
    39841 aatgcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc tgatttttag
    39901 cttactgt
//
LOCUS       DAZKGS010000023        39478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKGS010000023 DAZKGS010000000
VERSION     DAZKGS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            270..689
                     /gene="umuD"
                     /locus_tag="ACHOQY_001255"
     CDS             270..689
                     /gene="umuD"
                     /locus_tag="ACHOQY_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9677396.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            689..1956
                     /gene="umuC"
                     /locus_tag="ACHOQY_001256"
                     /pseudo
     CDS             689..1956
                     /gene="umuC"
                     /locus_tag="ACHOQY_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2002..2532)
                     /gene="dsbB"
                     /locus_tag="ACHOQY_001257"
     CDS             complement(2002..2532)
                     /gene="dsbB"
                     /locus_tag="ACHOQY_001257"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9677397.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2678..4219)
                     /gene="nhaB"
                     /locus_tag="ACHOQY_001258"
     CDS             complement(2678..4219)
                     /gene="nhaB"
                     /locus_tag="ACHOQY_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9677398.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4441..5160
                     /gene="fadR"
                     /locus_tag="ACHOQY_001259"
     CDS             4441..5160
                     /gene="fadR"
                     /locus_tag="ACHOQY_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9677399.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5212..6744)
                     /gene="ycgB"
                     /locus_tag="ACHOQY_001260"
     CDS             complement(5212..6744)
                     /gene="ycgB"
                     /locus_tag="ACHOQY_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9677400.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7062..8372
                     /gene="dadA"
                     /locus_tag="ACHOQY_001261"
                     /pseudo
     CDS             7062..8372
                     /gene="dadA"
                     /locus_tag="ACHOQY_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
     gene            8382..9452
                     /gene="dadX"
                     /locus_tag="ACHOQY_001262"
     CDS             8382..9452
                     /gene="dadX"
                     /locus_tag="ACHOQY_001262"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9677401.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9838..11574)
                     /gene="cvrA"
                     /locus_tag="ACHOQY_001263"
     CDS             complement(9838..11574)
                     /gene="cvrA"
                     /locus_tag="ACHOQY_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9677402.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11669..12583)
                     /gene="ldcA"
                     /locus_tag="ACHOQY_001264"
     CDS             complement(11669..12583)
                     /gene="ldcA"
                     /locus_tag="ACHOQY_001264"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9677403.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12683..13294
                     /gene="emtA"
                     /locus_tag="ACHOQY_001265"
     CDS             12683..13294
                     /gene="emtA"
                     /locus_tag="ACHOQY_001265"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9677404.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13296..14030)
                     /gene="ycgR"
                     /locus_tag="ACHOQY_001266"
     CDS             complement(13296..14030)
                     /gene="ycgR"
                     /locus_tag="ACHOQY_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9677405.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14231..14485
                     /gene="ymgE"
                     /locus_tag="ACHOQY_001267"
     CDS             14231..14485
                     /gene="ymgE"
                     /locus_tag="ACHOQY_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9677406.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14663..15103
                     /gene="ycgY"
                     /locus_tag="ACHOQY_001268"
     CDS             14663..15103
                     /gene="ycgY"
                     /locus_tag="ACHOQY_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFP9677407.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15182..16879)
                     /gene="treA"
                     /locus_tag="ACHOQY_001269"
     CDS             complement(15182..16879)
                     /gene="treA"
                     /locus_tag="ACHOQY_001269"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9677408.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17199..18617)
                     /gene="dhaM"
                     /locus_tag="ACHOQY_001270"
     CDS             complement(17199..18617)
                     /gene="dhaM"
                     /locus_tag="ACHOQY_001270"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9677409.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18628..19260)
                     /gene="dhaL"
                     /locus_tag="ACHOQY_001271"
     CDS             complement(18628..19260)
                     /gene="dhaL"
                     /locus_tag="ACHOQY_001271"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9677410.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19271..20341)
                     /gene="dhaK"
                     /locus_tag="ACHOQY_001272"
     CDS             complement(19271..20341)
                     /gene="dhaK"
                     /locus_tag="ACHOQY_001272"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9677411.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20569..22488
                     /gene="dhaR"
                     /locus_tag="ACHOQY_001273"
     CDS             20569..22488
                     /gene="dhaR"
                     /locus_tag="ACHOQY_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP9677412.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22588..25473)
                     /gene="ycgV"
                     /locus_tag="ACHOQY_001274"
     CDS             complement(22588..25473)
                     /gene="ycgV"
                     /locus_tag="ACHOQY_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9677413.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26314..27405)
                     /gene="ychF"
                     /locus_tag="ACHOQY_001275"
     CDS             complement(26314..27405)
                     /gene="ychF"
                     /locus_tag="ACHOQY_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9677414.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27522..28106)
                     /gene="pth"
                     /locus_tag="ACHOQY_001276"
     CDS             complement(27522..28106)
                     /gene="pth"
                     /locus_tag="ACHOQY_001276"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9677415.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28384..28662
                     /gene="ychH"
                     /locus_tag="ACHOQY_001277"
     CDS             28384..28662
                     /gene="ychH"
                     /locus_tag="ACHOQY_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9677416.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28717..30396)
                     /gene="dauA"
                     /locus_tag="ACHOQY_001278"
     CDS             complement(28717..30396)
                     /gene="dauA"
                     /locus_tag="ACHOQY_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9677417.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGHLAFFPNRRAAMADL"
     gene            complement(30521..31468)
                     /gene="prs"
                     /locus_tag="ACHOQY_001279"
     CDS             complement(30521..31468)
                     /gene="prs"
                     /locus_tag="ACHOQY_001279"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9677418.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31619..32470)
                     /gene="ispE"
                     /locus_tag="ACHOQY_001280"
     CDS             complement(31619..32470)
                     /gene="ispE"
                     /locus_tag="ACHOQY_001280"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9677419.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32470..33093)
                     /gene="lolB"
                     /locus_tag="ACHOQY_001281"
     CDS             complement(32470..33093)
                     /gene="lolB"
                     /locus_tag="ACHOQY_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9677420.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33307..34563
                     /gene="hemA"
                     /locus_tag="ACHOQY_001282"
     CDS             33307..34563
                     /gene="hemA"
                     /locus_tag="ACHOQY_001282"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9677421.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34605..35687
                     /gene="prfA"
                     /locus_tag="ACHOQY_001283"
     CDS             34605..35687
                     /gene="prfA"
                     /locus_tag="ACHOQY_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9677422.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35687..36520
                     /gene="prmC"
                     /locus_tag="ACHOQY_001284"
     CDS             35687..36520
                     /gene="prmC"
                     /locus_tag="ACHOQY_001284"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9677423.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36517..36909
                     /gene="sirB2"
                     /locus_tag="ACHOQY_001285"
     CDS             36517..36909
                     /gene="sirB2"
                     /locus_tag="ACHOQY_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9677424.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36913..37722
                     /gene="sirB1"
                     /locus_tag="ACHOQY_001286"
     CDS             36913..37722
                     /gene="sirB1"
                     /locus_tag="ACHOQY_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9677425.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37758..38612
                     /gene="kdsA"
                     /locus_tag="ACHOQY_001287"
     CDS             37758..38612
                     /gene="kdsA"
                     /locus_tag="ACHOQY_001287"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9677426.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38761..38868)
                     /gene="ldrA"
                     /locus_tag="ACHOQY_001288"
     CDS             complement(38761..38868)
                     /gene="ldrA"
                     /locus_tag="ACHOQY_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9677427.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39296..39403)
                     /locus_tag="ACHOQY_001289"
     CDS             complement(39296..39403)
                     /locus_tag="ACHOQY_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9677428.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
BASE COUNT         9789 a         9844 c        10158 g         9687 t
ORIGIN      
        1 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
       61 gaatgcttct attctatttt ttagccgggt aatatttttc atttctgctg gatgagcgta
      121 gtcgccagaa ggccgcgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
      181 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac tactgtatat
      241 aaaaacagta tagcttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
      301 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
      361 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
      421 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
      481 tactgattgt cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg
      541 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
      601 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
      661 tgatccacgt cgttaaggcg atgcgctgat gtttgctctc tgtgatgtaa acgcgtttta
      721 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
      781 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gtgttaaaat
      841 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
      901 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
      961 atcgccccgc gtcgagattt acagtattga tgaggcattc tgcgatctga caggtgtgcg
     1021 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     1081 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ctggctaagc ttgccaatca
     1141 tgcggcaaaa aaatggcagc ggcagacggg ggggtggtgg atttatcaaa tctggaacgc
     1201 cagcgtaaat taatgtctgc tctccccgtg gatgaagtct gggggattgg acggcggatc
     1261 agcaaaaaac tggacgcgat ggggatcaaa accgttcttg atttggcgga tacagatatc
     1321 cggtttatcc gtaaacattt taatgtcgtg ctcgaaagaa cggtgcgtga actgcgcggc
     1381 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     1441 tcgtttggtg aacgcatcac ggattatcca tcgatgcggc aggccatttg tagttacgct
     1501 gcccgggcgg cggaaaaact tcgcagcgag catcaatatt gtcggtttat ctccacattt
     1561 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa
     1621 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     1681 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     1741 agtcagggag tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag
     1801 caattgatga cggtaatgga tacactgaat gctaaagagg gcagaggaac actctatttt
     1861 gccgggcagg ggatccagca acaatggcag atgaagcgtg ccatgctttc accacgttat
     1921 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct
     1981 cccgcaggag cgccgaatgg attagcgacc gaacaggtca cgttttttcg ctttaaacgg
     2041 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     2101 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     2161 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     2221 acaggtggca aacggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     2281 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     2341 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     2401 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     2461 tgccagagca gtaaacgcca tcaacaacca cgcgccccgg ccttgtgaac attggttcaa
     2521 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     2581 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     2641 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     2701 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     2761 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     2821 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     2881 cgacggaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     2941 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     3001 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     3061 catgttccga tgcctgcaac acaaactgaa taattggcga aaacagttgt tggtcgataa
     3121 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     3181 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgtcgcc agaataatga
     3241 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     3301 cgccaataat cgcctggaca atcagacgga ttttatcctg acgagtacgc tggtggcggc
     3361 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     3421 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     3481 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     3541 tgatcaggtt ctgtggttcg cccaccatgg tcatcacgcc gcctaatgca gtgccgacac
     3601 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc actttgtaat
     3661 gcttgtcgat atgactatcg tcttgcaggt cggtatcttc agtacgggaa gaggctacgc
     3721 ggtgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     3781 catcgaggaa cgcggagagg aacgccgccg ccacgcaaaa agagagcgac agcagcattt
     3841 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     3901 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt
     3961 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     4021 cggggagcag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     4081 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     4141 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     4201 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta
     4261 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     4321 aaagagatgc ggaaaatgaa gccttgatcc cttttttctt ctttttgtct gctatcagcg
     4381 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     4441 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     4501 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     4561 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     4621 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ctctggttta
     4681 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     4741 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     4801 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     4861 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     4921 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     4981 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     5041 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     5101 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     5161 tcccttccgt ttaaagagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt
     5221 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     5281 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc agcacttctt tgcgcccccg
     5341 atccagcggt gcgcgattat gtggaatgta acgcagcgtc agcgaacggt cgccgcgcaa
     5401 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     5461 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     5521 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     5581 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     5641 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     5701 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     5761 ccacgggctg ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag
     5821 cataaaacgt tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca
     5881 gaaggtcgcc cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct
     5941 caccttacgc acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt
     6001 ctccataaaa tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac
     6061 cgttttctct tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag
     6121 atactcttcg cgacttttct gccgggcttt ctcttcttgc agtgagattt tttgcgggcg
     6181 tttgtaccgg tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac
     6241 ttcatcaacg ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata
     6301 atcgacaatc gaactggcgt cggtccaact gcggaataag taattgtttt taaagaaaga
     6361 gttatgccca tagcaggcgt gtgccatcac cagcgcctgc atggtaatgg tgttctcttc
     6421 catcaggtaa gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg
     6481 accgtgctta tacagccgtt cagtttcgat aaacttttta ccgaatgacc agtgcggata
     6541 gttaattggc atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc
     6601 aatctggtgc gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag
     6661 ataaacatcc agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt
     6721 attcatagaa tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg
     6781 agcacctcat ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc
     6841 tttgcgattt cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg
     6901 ataaaaaggg cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga
     6961 tgtgagccag ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca
     7021 agatgtaatt agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag
     7081 ttgtcatact gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag
     7141 gacatgaggt caccgtcatt gatcgggagc cgggggccgc cctggaaacc agcgccgcga
     7201 atgccgggca aatctccccc ggatatgctg caccgtgggc ggcaccaggt gtgcctttaa
     7261 aagcgatcaa atggatgttc cagcgccatg cgccgctggc ggtccgtctc gacggtacgc
     7321 agtttcagtt gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg
     7381 aaaacaaagg gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc
     7441 gcgccgaaac caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta
     7501 ccgaacaaca gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac
     7561 cgtatcagct gctggaatcc agccgcctgg cggaagtgga gcccgcgctg gcagaagtag
     7621 cgcacaaact gacgggtggt ctgcaactgc ccaatgatga aaccggagac tgccagctgt
     7681 ttacccagaa tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc
     7741 ccgttgacca actgctttgc gacggcgagt aaatctacgg cgtgaagtgt ggcgatgaag
     7801 tgattaaggc cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag
     7861 gcattgttga tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc
     7921 aggaagatgg tgcgccagta tccactattc ttgatgaaac ctacaaaatc gccattaccc
     7981 gtttcgataa ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc
     8041 tgttgcaacc gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg
     8101 gtcatgtcga gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc
     8161 cggttgtcgg gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg
     8221 gctggacgat ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc
     8281 cagcgatccc atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac
     8341 gtccgggcca tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg
     8401 ccagcctcga tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc
     8461 acgcgcgcgt ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct
     8521 ggagcgcgct cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt
     8581 tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg
     8641 atctggagat ttatgaccag caccgcctga ccacctgcgt acacagcaac tggcagctca
     8701 aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg
     8761 ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag cagttgcggg
     8821 caatggcgaa tgttggcgag atgaccctga tgtcgcattt tgccgaagcg gaacatcctg
     8881 atggaatttc cagcgcgatg gcgcgtattg agcaggcggc ggaagggctg gagtgtcggc
     8941 gttcgttgtc caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc
     9001 ggcctggcat tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata
     9061 ccggattacg tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag
     9121 cgggtgagcg tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca
     9181 ttgtcgccgc agggtacgcc gacggttatc cgcgccacgc gcctaccggt acccctgttt
     9241 tagtggacgg cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctagcggtcg
     9301 atttaacgcc ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga
     9361 tcaaaattga tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc
     9421 tggcgttacg cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggaggcagcg
     9481 tcttctggtt gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga
     9541 cagagccatt gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg
     9601 aactcaccgc acaggcgttc tacataaaac gcttacgctt cattgttgac tcgaactcga
     9661 cttcagataa atcacgcttt acccttgatg gagcctgtac atagatttgt gtaattgcct
     9721 gattttgata tgttcaattc aacatcaaat gaaggttaaa ttatggacga caaacaattg
     9781 caggctcagg ctgcgttcag caaagcatcg caaccggcga tagatgcttc attaaattta
     9841 agattcagct tcctcttcag ccacccgtac gccaatcttc aacacttcat tatcttcttt
     9901 ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa ccggtgccgc
     9961 gcctaacaac tgctggacaa tttcacccag cgtttgctgc ttatcacgat attctcgccc
    10021 gtcttccagg ccatatatca gcgcaacatc agcatatttg gcgctggctt cgagaatgaa
    10081 gtcaccaaag aagcgttgat ccagcgcgac cggcggcgac tggctgaaca gtttaccgag
    10141 cgccggaaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt
    10201 gctgccggta ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt
    10261 tggcatatgc aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac tcaattgata
    10321 aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg acaccggacg
    10381 tcccactggc ggaaccacca ctttggcttt tttagccgcc cacgagagtg atgttccctg
    10441 caagagcagt gaaaccagaa ccacaaagaa ggcgacatta aagaacagac gtgcattctc
    10501 cagccccgcc atcatcggga acacagccag gatgattggc accgcgccgc gtaatcctac
    10561 ccagctgata aacacgcgct cacgcagatt gaaaccgcgg aaggggagca atccagcaaa
    10621 taccgaaagg ggacgggcga agaatatcat ccatgccgac aaaatgagcg ccggaatggc
    10681 aataggcagc agatcgcttg ggttaaccaa cagccccagc accaggaaca tggcgatttg
    10741 cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg
    10801 attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt
    10861 ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata atccggcggg
    10921 cagggcgatg cgattaatca tttgcagcag taaataacca ccaccaagtc cgatgacaat
    10981 cccgaggcca aattgctgca gaatatcgac cacgaacatc caactgacac tgctttcatg
    11041 ttgctggatc atcgcaatca gggtaatcgt cagaaagacc gccattggat cattactgcc
    11101 ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac ccagcagaga
    11161 aaagaccgct gcagcatcgg tagagccgac gatagcgccg attaataagc cttcaatcaa
    11221 atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga taagcacgcc
    11281 cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg cctgagtgcg
    11341 catcccgccg tcgagcaaaa taatcgccag tgccaggtta cttaccatgt aggcgaaggg
    11401 gtaattatca aacgggatac cgccgacgcc atcgactcct gccagcatgc cgatcgccag
    11461 aaaaataacc agaataggaa tgccaagacg ggaagaaaat gaactaagta atatactgct
    11521 ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg
    11581 gttactcctg attacgctgt ctcttatata aaccctacca tattagcggc agagacagcg
    11641 ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg
    11701 tcaactgagt gccttcccgg gtgttattca gaatggcatg tgcgcccaac ggcagcgtaa
    11761 ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac
    11821 gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcattg ggcgtgctgc
    11881 cgctaaagct accgagaata atcgccttct gacgtggcaa aattcccgca tgatagagct
    11941 gcaacagcat acgttcgacg cggaaagggt gctcgttaat atcttccagc accagaatac
    12001 cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc gcaagattgc
    12061 ctccccacaa cgtgccttcg gtctgacatg tcgggccttc cccttgccat tcaatggtga
    12121 aggtttcatt gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc agttcatccg
    12181 cgccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct tgcgccagaa
    12241 gaccgcactg aatggcggta aaatcgctat gcccgcatat gagcaacggg tcatattgtt
    12301 ggcgagcaac cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc
    12361 cgcgtacagc cagcacgatg gtgttggggg tggtcagtct ggcaagggaa ttgagatctt
    12421 ccaggcgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt
    12481 tattgacctg atgcccctcg tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt
    12541 taatgcagta acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac
    12601 aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt
    12661 ccaggctata aggatagaag aagtgaaatt gagatggttt gcctttttga ttgtgttatt
    12721 agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga aagttccggt
    12781 gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga
    12841 tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga atgcggtgag
    12901 taaatcgaat gccattggtt tgatgcagtt aaaagcttca acctccggac gtgatgttta
    12961 tcgccgtatg ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc cagagcgtaa
    13021 tatttcaatg ggggcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga
    13081 agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg caggtgcgct
    13141 gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atttggatgc
    13201 tgacgagttc ctcgatcacg tagcgcgaaa tcatcctgcg ccgcaggctc cgcgctatat
    13261 ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cgcgcacttt gtccgctttt
    13321 tcccgggctt ctcgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg
    13381 ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt cttgccatca
    13441 atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac accccattgc
    13501 cccatgttga cttcaatctg agcgaagcgc atgccttctt gtaattcggc aggctttgct
    13561 gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta
    13621 ctgttatccg ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggagatgcgg
    13681 aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtgat aaatgccgga
    13741 agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt cgcaccctga
    13801 gtttcggcgg taatggtaat gtgctgtgcc tttagcacgg cgatgttgtc ttcggcttga
    13861 ctgccgaaat ccagcaccag tttatccggg gttattgcca gtattttgct gatcagctgc
    13921 ccgccattcc aactgagacg caaaggaatt gcggctttgt gcaaatcgcg taacacgccc
    13981 aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac
    14041 tcctgactga caaactgtct ttggtttatc ggtcacagtt aacaaaactt aatacaaatg
    14101 cgtgaatatt ttttacatgt tgttcttaaa tcagccgaca tacgcccagc atcgatcccg
    14161 cccttgccta tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga
    14221 gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg gcattatcgc
    14281 caagctaatc atgccggggc gtgatggtgg tggatttttc ctgacctgta ttctcgggat
    14341 agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccatcag
    14401 tggttttaat ctgcacagct tcctggtggc ggtggtggga gctattctcg ttctgggcgt
    14461 attccgcctc ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc
    14521 tatccgcttt cacatcagaa cgtatactcg acacctgctt tacgggtgga acaaatcaat
    14581 atagcactta gatgcccgat aaagtcggtt cccggtaaca acaacagaat ccttttttaa
    14641 ttattgtttc gttgtttttg gtgtgattca gaaaataaat attttagaca gtagccagtc
    14701 aggcgttaaa aatggggcgg atagtgaggc ggttttggct gcgcttattg aacatttaga
    14761 attgatcaat ccttctggca gattaagcca aaatacccgt tcggcaatgc ttcagttacg
    14821 cgaagaggaa tggtctgaat tctttttttg gttgctaaat tctctggagt gtctggatta
    14881 tgtgattatt aatttagctc cagaaagcaa aaaaacgttg atgagtgaac accgtaacaa
    14941 tattcaggta gcaatcgatg ctttatatag gcaacgtcgg tgtaaatcac cgggtgatga
    15001 aagtgaaaca cttacccgta gaaatgacgc tatcttcggc aaccacgtat ggcaaacatt
    15061 tgcgcaatac ttcccgccag gacttgaaaa accgtctgtg tagaaaacac catagctatg
    15121 gtgttatctg ccgacaacgc catccggaga agccgaatga cgttgtcgga gtaagcgctg
    15181 gttaaggtgt gggttgtgcc tctttggttg agggttgcgt cgttgctgac ttaacggtcg
    15241 gacgcgtcgc cggaacattg tcacacggtt gctctttcgg gcagatcaaa tccagcattt
    15301 tcagcgtcac gccattggtc cagccaaagc catcctgtaa tggatattcg ccaccgccgc
    15361 cccccgttcc ggtggtgctg acatcatatt tttccaccag ctttttctcc cggtcatagg
    15421 tgtgctgaac attggtcagg aagtgccagc taatgtccat cgccacctct ttttgcccgt
    15481 agttttgtaa tccttctgtc gcgacccact gtaacggtgc ccagccattt ggcgcatccc
    15541 attgttgccc acttttcacc gacgtggtgt tcaggccgcc gggttgcagc agatgtgttt
    15601 tcgtcgccgt cgccattttg ttggcgcgat ctttcgctgc cgcattgacg tacagcggga
    15661 acagggcggc cgcggttaac tgattgcgca ctttatgact tttcaggtcg taatcggcat
    15721 accagccttg ttgatcgttc cacatgtatt tttcgatccc tttttgacgg gcatttgcca
    15781 gcgtttcgta ctggtttgcc atcgcgttat ctccggcagc tttgctggcg cgggcgagga
    15841 ttttttccat tttaaacatc aggctgttca gatcgaccgg tacgatgctg gtggtgcgta
    15901 aggtatttag ttgatgcggg ttgtccatcc agcgcgagct gaaatcccag ccagacgcag
    15961 cggcagagcg caggtcgcgg taaatttcag tggcaggtcg attcggattg cttttggcgg
    16021 tggcaatatc ttccacccat gactctggtc gtggcgtatc gcgatcgtcc cagtagcggt
    16081 tgagaagggt accatcctga agttttacaa cgcgtttttc ctgttgtccg gcttgcaggt
    16141 tttcaacacc gtccatccag taagcatatt ctttttgcat ttgcggcagg tattgcttca
    16201 acgcggcatc gccttcatgc tgcgccagta actctaccat cagggcaaag aagggcggtt
    16261 gcgagcggct taaatagtaa ctgcggttgc cgttgggaat atgaccgtaa gtgtctattt
    16321 catgagcaaa attggccacc atatccgcga ctttatccca gtgaccgctt tcggcaagtc
    16381 ctaacatggt gaagtaactg tcccagtaat atacctcgcg aaaacgtccg cccggcacga
    16441 cataaggttc cggcagcggt aacagagaat cccatttttc ggtgttttcg gtagaacgcg
    16501 ttaataccgg ccaaagtccg tcaatatgtt cgcgcagtga ctgcccctct ggcggaacat
    16561 atttctcgcc ctctttcggc agggtgaaat tgacgttaac gaaatggcgc agatcaaatc
    16621 cgctctggtt ttgctgcatc cgataatcag caaggatcat cagcggatcg ctgttcggca
    16681 cggcatcggc aaaggttttt tggtccggaa aaagtttggc gttttgcaca tcattaaaca
    16741 gcggccctaa taaaatatca ggcggctgtg gtgttaccgg tgtttcttct gcctgcaccg
    16801 atagcgcagc gaaacacaaa aagatacagg ctggaattaa cgccattttt tgcgggcgag
    16861 aaggtgcggg ggatttcatc aatcattctc ctttggcgaa accgaataaa acggtattca
    16921 agtatcagaa aaccttagtt caggatcgca ctagctgcat gatcgaaatc tcattctgcg
    16981 aactgtcagg cattttagaa aagggaatta aagggataaa taacggtgaa atagagtgat
    17041 caaataacgt ctgtttatta gccagtcagc gacgttgggt tcgcgattag cgaagaaaaa
    17101 tcatcataaa ttggtaaaaa atataacagg tagttaaaaa acatcagtgc tgggtaaatt
    17161 gccggatgac atcagaacga tgccatccga acggtggctt aaccctgacg gttgaaacgt
    17221 tgcgttttaa cgtccagcgt tagcgtttct tctggttgta tcgcatacag tttctcaccc
    17281 tgctggcaaa tccagccaat ccccagttca cgggcgatta gggcgctgtg ggataccgga
    17341 cttccggcgc taaggcagat accttttaca accgccggat ccagttgcag tactgtggaa
    17401 ggataaatgt tctccgccag tagaatagtt ggcgagttaa actgcgggag ttcttctttc
    17461 gtttgggtca ggtggaccag ggtgcgatgc agaagatcgt ccacatcaat atagcgagct
    17521 tgtagatatt catcatccag ttgctggtat tgctggctaa gttctttaag aacttgctgc
    17581 caggcatatt ctgccgtgca atgttcatgc tgaaggagtt cgcttgccgc cgccagcagt
    17641 tccggatcat ctaacagtgt atggtgacca gaaaagattg cggcaatatc gtcaagcccg
    17701 ctggcttctg ctttcgctgt taacgtcatc agatctaata acgtgaagtc aatagcctgg
    17761 cgtaatcgat cttgttcttc ttccacggtc agggttgatt ttgcctgtac cgtacataaa
    17821 actggttgat aataaaaggc tttacccgaa acaggcggaa cgggacgcag agtaggtgga
    17881 gcgacttcct ccgtttcacc aaagttatct tcagccagct gacggaaagc gatcagtgcc
    17941 tcttcagctt ctggcccttt cgcaatcagg cgcagcgtat cgttatagcg aacttgtagt
    18001 aacgcaatct ggttaatact ctctggtgtg acgcatttgc cgtttttttc cagcaacata
    18061 tcggcattaa atgtcgataa ggtataaacc agccgggagg ccggacgtac atgcaggccg
    18121 ttacggtttt ttatgaccac cgccagagaa cgggcttctt catcgtacgc aggacatgtg
    18181 tcagagattt cagtgtcgga ggacggtaaa cccagttgtt cacgtttggc ttccagcgca
    18241 tgcatagcgt caaagataac tttgtcgata tccgctcccg aggccgcgct gaccgttgct
    18301 gccagtgtac cttcgaccaa cggcgcagca cacaaacgta cttttgcggc gatcttggga
    18361 gccagcaatt ccagcgcagt ttcagcactc aataatgcgc tacccatatc catcatgacc
    18421 agcacatggt cggcatcagc aacagattcg atggcctcca tcactttgac ggcatcggta
    18481 ccaataggat tttgtggatc gtcaattccc gcggcaatgg cgattttaca actatcactc
    18541 attaacatct gacgggctaa ttcaccgaca ccttctccca gtcggctgct atgtgaaact
    18601 atgaccaggt ttaccatcac caattcctta ctcttttgcg gctaacgcca acatttgcat
    18661 cataaacatc accgaggtcg cgccgggatc ctggtgacca atactgcgtt caccgagata
    18721 actggcgcgg cctttgcggg cttgcatcgt aatcgtactt tgtgcagcgg attcggcgat
    18781 tctactggca gcttcgagcg ccaccggaac agagagattt tgctcgctgg actgacgtaa
    18841 cgattccacc accggcaccc acacatcaca catggtttta tcgccaggtt cggctttccc
    18901 gcgactgatt acgccgtccg cgccatcgcg gaacatctga taaagctctt ccagtgtcag
    18961 gctttgccgt gcctgggtcg cctgtgcggc gcggataaag aaggtaccga acagcggacc
    19021 actggcacca ccgacgctgg aaagcagcgt cataccggta ttcttgagaa tgaaaccgat
    19081 atctttatct gcgatagcag ggagtttttc caccactttg ctaaagcctc ggttcatatt
    19141 tagcccgtgg tcagcgtcgc caatttcgcg atccagtccg gtaagatact cgctctcggt
    19201 gctgaaaata tcgccacaac gagtgagcca gttaacaatt tgagttctgc tcagtgacat
    19261 tgctttctcc ttatttaccc cagttaaggg ccggggtgtg gaccggggcg tcccagagtg
    19321 ccagcgtttc gtcatcaact ttcagtaagg tgattgagaa accggtcata tccagtgagg
    19381 tgcagtacgc gccaattaaa ttacgttcga tagtcaatcc cgcttgctgg caacgtgtgg
    19441 tcaggcggtt atagacgccg tacagctcag aaagcggagt tgcgccaaga ttgttaacca
    19501 gcgcaatcac ccgatcgcca gactggagcg gttgtttggt ttgttgttct tcctgccaac
    19561 tgccttgttg ataatcccag aaacgcaaag tgcgatggta tgagccattt tccagcaggg
    19621 tgtcgaacat ttcatcgacg gtttgatcaa gggaagagaa ggagcggcgg tcaatacccg
    19681 gctcaccatg aatgccgacg ccaaactcca tctcattatc cgccagggta aaagaaggtt
    19741 tgcccgcggc aggaacggta caggcaccga gagcgatacc tattgagtgg ccttgattat
    19801 tcaacttacg ccccagttcc gcacaggcgt ccagtgagtc gccacgctcc gccgctgcgc
    19861 ctacgagttt ttcaattaat acggtgttgg caacgccgcg tcgcccggcg gtataaagac
    19921 tgtcttttac cgcaacgtcg tcatcaatga ccacagtggt cacttttacg ccgctatcgt
    19981 gcagtaactc ggtcgctgtt tcaaagttaa gaatatcgcc ggtgtaattt ttgataatca
    20041 acagtacacc ttcgccgcca tcaatttgca tggagcattc aaagatttta tcgggcgtcg
    20101 gtgaggtgaa aatttcgccc ggacaggccc ccgaaagcat cccctgaccg atataaccac
    20161 agtgcatcgg ctcgtgtccg ctgccgccac ccgacagcag ggcgactttt cctgcaacag
    20221 gggcatcagc tcgggtgaca tacaccggat cctgatgcag tgtcagcgat ggatgcgctt
    20281 tcgccagtcc tgccagttgt tcgtccagta cgtcttgcac atcattgatc aattttttca
    20341 ttattttgct ccagcaatta cggtagggca tggatgatgt tcaacgacac ggcgaccact
    20401 gactgccgat gaatccattg tgcatcaggc aagggaaaag aaaattagcc caaattatgt
    20461 ttcatagtga aacatatgct ttaatgaatg ttccatattg aaacttttac gtgtattaat
    20521 acttaaaatt gcgagccgga acaccttttg tcataaggga tgcgggatat gagtggcgct
    20581 tttaacaacg atggtcgggg catatctccc ttaattgcaa cctcctggga gcgatgcaat
    20641 aagctgatga aacgggagac atggaacgta ccacatcagg cccagggcgt gacatttgct
    20701 tctatttatc ggcgtaagaa agcgatgctg acgctcgggc aggctgcgct ggaagatgcc
    20761 tgggaatata tggcaccgcg agagtgtgcg ctgtttatcc tcgatgaaac cgcctgcatt
    20821 ctcagccgta atggcgatcc gcaaaccttg cagcagctaa gtgcactggg attcaatgac
    20881 ggcacgtatt gcgccgaggg aattattggt acttgtgcgc tatcgttagc ggctatctct
    20941 ggtcaggccg tgaaaacgat ggccgatcaa catttcaaac aggcactctg gaactgggcc
    21001 ttttgtgcaa cgccgttgtt tgacagcaag ggccgattga cgggaacaat agcgctggcg
    21061 tgtccggttg agcaaactac cgcagctgat ttgccgttga cgttggcaat cgcccgcgag
    21121 gtcggaaatt tactgctgac ggacagtttg ctcgctgaaa ctaaccgtca tttaaatcaa
    21181 cttaatgccc tgttagaaag tatggatgat ggcgtgatta gctgggacga gcagggtaat
    21241 ttgcaattta ttaatgccca ggcggcgcgg gtcttgcgcc ttgacgcgac ggcaagtcag
    21301 ggaagggcaa tcactgaact cttaacgtta cccgccgtat tgcaacaagc aataaaacag
    21361 gcacatccgc tcaaacacgt agaagcaacc tttgaaagtc agcatcagtt tattgatgcg
    21421 gtgataaccc ttaaaccgat aatagaaacg cagggaacca gctttatttt gttgctccat
    21481 cctgtggaac agatgcggca gttgatgacc agtcaattag gaaaagtcag ccataccttc
    21541 gctcatatgc cacaggacga tccgcaaacc cgccgcttga ttcattttgg tcgccaggcg
    21601 gcgcgcagta gctttcctgt cctgctttgt ggagaagagg gcgtgggaaa ggcactgcta
    21661 agtcaggcaa ttcataatga aagcgagcgt gctgcaggtc cttatatcgc cgtcaattgt
    21721 gagttatatg gtgatgcggc gctggcggaa gaatttattg gtggcgatcg cacggacaat
    21781 gaaaatggcc gtctgagtcg gctggaactg gcacacggcg gcacgctgtt tcttgaaaag
    21841 attgaatatc tggcggtgga gttacagtct gctttgcttc aggttatcaa gcagggtgtt
    21901 atcacgcgac tggatgcgcg gcgtttaata ccagttgatg tcaaagtgat tgccacaacg
    21961 accgcggacc tcgcaatgct ggtggaacaa aatcgtttta gtcgccagct gtattacgcg
    22021 ttgcatgcat ttgaaattac catcccgcct ctgcgtatgc ggcgtggcag cattccggcg
    22081 ctggtgaata acaaattacg cagtcttgaa aaacgcttct ctacgcggct gaaaattgat
    22141 gacgatgccc tcgctcgcct ggtttcctgt gcatggcctg gcaacgattt tgaactttac
    22201 agcgtcatcg agaatcttgc tctgagtagt gataatgggc gcattcgcgt cagtgatttg
    22261 ccggaacatc tgtttaccga gcaggcaaca gatgatgtca gcgccacacg cctttccacc
    22321 agtctgtcat ttgcggaagt tgaaaaagag gcaattatta acgcagccca ggtcacaggc
    22381 ggtcgcattc aggaaatgtc ggctttactt gggatcggcc gcactacgct gtggcggaaa
    22441 atgaagcaac atggcattga tgcagggcag tttaagcgcc gggtatgaaa gacagagacg
    22501 atttctgata catcagagtg atctatattt cattccggcg cacgctaaca tttttcagca
    22561 tcgtttaagg gctggtctat cccgcactta aaagctgaag cgatatcctc cgttgacttg
    22621 cttctgatca aagaggttac tctgcgtgta atccgcttcg aggtagaatg tgtgctgttt
    22681 gttatactgt gcactgactc ccacgccgtt attccagcca ttacctttga aactgtactt
    22741 ctcccgggag ttgttcaaca gatagtctgt atcccctgaa aactcgcgga tagctccagt
    22801 cttcacatac atattcagct ggctgttgcc tgcggtgatg tcatatccca gtatcatgct
    22861 ggcacgcccc agcagcgatt cgtagtgatt cagatgtata ttgagaccat tactcgcctt
    22921 catagccatc tcattctggt ggctgtatgt gagctgggtt tgcggctcta tatagaaccc
    22981 ataaccagta ggggtcaggt tgaacctctg cccggcttcc agggagatgc tcaatccatt
    23041 cgcagtgccg ttggcgttaa cgccgttgtt ctgactgtcc agtacgtgaa aactattttt
    23101 ctggcgcgat gcttttacaa cgagatcgct gtaaaaaccg ttgtgtgcca tgtaactggc
    23161 atacattccc atgtagtctg aacgtgcggt accgtcgcct ccgctatagt ccggcgatgc
    23221 atgtgttgag ccaatataca gaccgacata caacggcatt acatcagaga gacgtttatc
    23281 cccaccaaac tggataccgc tgtaacccat gtcaaagccg ctcagtttgc cactggcgaa
    23341 ggagtccagg cttcccccat aactgcgcag ccagatatta ccgtctttac tctgatttcg
    23401 caggtcaccc attcgttgca tcagcgtacg gttttcaaca tagtttaata agtaaccgac
    23461 attgagataa ttgccgccag catctgcggt cgtggtgggt ttaggtgtgg gagtggggtt
    23521 aggcgtagga tcgggttcag gcgtaggatc ggggttgact atgggaggct gagcgggagc
    23581 cggtgttggt tcaggattag gagtcggttc cggaactgtc ccggaagcgt aaagctccca
    23641 gttagtgcca tttttacgca catcgtacag atatcccccc aactcaacct gagaagacgc
    23701 gctgaacgag gccgcaccgt cagtggtttt taccactgtc agaacttcat ttcccgtagt
    23761 ggcctcgctg ccctggttgc ggatagccaa tacgtgatta ccagcactac ttccgctgat
    23821 attcaataaa tcccctttat tattaacgcc attaccctcg ccaacaacat cagcacgcat
    23881 aataaaggta ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg tgccggcagt
    23941 tgacccgtgg ctggcaaaat cgacagttga atggctcagc gccagcgtgt cgagattaga
    24001 attacttgtt acgttccaga cgctgttatt cattgcaacg tccagtttcc cgccattgac
    24061 attatcgctg agggaggaac ctgtccaaac cgaccctggg tgcatatcta gattgattag
    24121 cccacctttg gaaagaacgc taccgttgat aagcatacga cccgaggcgt tgatgcggct
    24181 ggcggcataa ccatcgtcat gttgcgttgc aatggccatc tggtcgggtg tactcatatc
    24241 aatgaccaga tcgctcgtga ggtcgatctg gctgttggtc atggcataaa ttcccctggc
    24301 tcctgccgcg ccggtgatag ccaaactgtc tccggttatt ctaccgccgc tgagcgccca
    24361 caaacccagc gccagactgc cattacgatc aactgtaata tcggtatttt gcatgttgac
    24421 aacagccgtt gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga tgctgtttcg
    24481 ttgcgctgcc gtgccagaaa aattgattgt cgcgtctgaa ccactggtga cgaggccacc
    24541 gccctgcgcg gaagaaatat ggctatctgc accgatagtg gttgtaccac cacgaacttc
    24601 gacgccattg gccgcggctc cagttacatc aacagtgagt gcattcgcgc taacctggcc
    24661 aaagctccag aggccgtgtg cattatcgcc attggtttta atggaactgt ttgttccgag
    24721 atcgacaaca gagtttttct gtacgtttat ccccatggcg ctgtagccct gaacatcgat
    24781 tgtcaggtct gtagccgtaa aacgcgcagc accattggcg ttattgccgt tgagaccacc
    24841 gatataaaca cctgtacttc catcggtcgt gattttactt ccgcttccaa gatcgacact
    24901 ggtgccatag tcattgatgg ttaggcctat accgttcgag ttttcaatgg tgaattgagt
    24961 ggctgttagt gttgagctat gcccaataat gatgccgtca tcgttactct taatggtgct
    25021 gcctgtgcct aagtcagcat gggtatagtc accaattaag ttaatgccga tggcagaggt
    25081 ttgaccaaca acatctactg tgagtcggtt agctgtcagg gagctgtttt tgccctgaat
    25141 aatgattcct tttgcatatc cagaggcgtc gttgacattg acggtgacat cattacccag
    25201 gttgatattg ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt ttcctgatgg
    25261 atcggcggta tctcctgtaa ttctgtcacc ttcattaata gtaatattgg tgccatattg
    25321 acttcctgta tcagcagcta tcagcagggg ggaaaagata attgcattca aaccaatagc
    25381 gccaaatttt atcagttgaa ttgagggcga acggattttt aattcagcga gacgatctgc
    25441 tttggtattc ccattgtgtt gtttgatgcc cataccactc ctatatagta cccagactat
    25501 gggtctatgg attttgtcat tttgtttaaa tcaatgaata atatcctctt atcatcatga
    25561 tatttgtatg gtgcaatcat gatgagcttc tcagagaata attctctatt accgggcaaa
    25621 atcaggaaca caggaattaa tatatattta acatccccct tacaaaggtg agtttatatc
    25681 gagttattcc ctaagttgtc gttgcttatt ccaattcgtt actataaata tggcaaaaat
    25741 attacaacaa acagcggtta aaaaatttgg tgaagatttt ttgtaataat cctaattagt
    25801 tatgggaatt tcgcttgatg tatctgcata acaaaaacaa tatgcgccat ttttgcatta
    25861 ctaataagaa aaagcaaaaa acatcgatat tcatttttgg tcaatgggtt ttctggccta
    25921 tcgttgttat tgtgctactg gttatcattt tcatcttgcc aggatcgcac gtaatcacgt
    25981 tattcgggga ctttatctgc gggatattac ccttcaggta agtccttttc tcgccagcat
    26041 attggagcgt gatcaatatt gatcagctgt gaagggggtg atgtgtagtg gaccaaaact
    26101 tacgttatat cactactaat gagatatccg tcatctgaca ctgatgtctt taattattct
    26161 cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg ttacaagaat gattaggact
    26221 cggtgaaatg aaaaatccac gcaattgcgt ggattatata tacttttgcg ctcttcatga
    26281 gatttagcga aacctcatga gacaacaaat taattagacg ttgaaaagga agttcatcac
    26341 atcgccatct ttaacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
    26401 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
    26461 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
    26521 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
    26581 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
    26641 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
    26701 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
    26761 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
    26821 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
    26881 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
    26941 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
    27001 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
    27061 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
    27121 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
    27181 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgttgcggtt ttacgatttc
    27241 agccagttga tccaggcgag ggtcaggcat tggtacgacg cctgtgttcg gctcaatggt
    27301 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
    27361 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
    27421 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
    27481 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
    27541 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
    27601 gcttcgtcga tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
    27661 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
    27721 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
    27781 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
    27841 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
    27901 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
    27961 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
    28021 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
    28081 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
    28141 gtagtttact ggttgcggcc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
    28201 ataattgcat gagtggacta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
    28261 tgttgaaata agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta
    28321 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
    28381 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
    28441 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
    28501 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
    28561 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
    28621 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
    28681 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
    28741 acgattcggg aagaacgcca ggtgtcccgg gatcggttga atgccagcgc gagccatagt
    28801 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccctcgg gcagacgctt
    28861 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaaccg catcccactt
    28921 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc
    28981 agcagcaaaa aacagcgggc caataacgcg cagaaccagg acatcgtctg gaacatctac
    29041 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
    29101 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag
    29161 cagcatgaca atgatgtcat ctttcggcgc atgacgcagc aagtcgacca ctttgtgcgc
    29221 ttcactcatg ttccacgcca ccatcaacag cagggcagcc atggcggaaa gcggcagcca
    29281 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
    29341 cgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
    29401 agcggtaatg ccaccaaaga acggagcgat gatattcccc agtccctgtc caaccagttc
    29461 gctattcgcc ttgtgtttcg tcccggtcat accatccagc accacggcgc agagcagaga
    29521 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
    29581 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
    29641 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
    29701 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
    29761 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc cagaaaacaa gaatacctag
    29821 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
    29881 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
    29941 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
    30001 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaaaatccc
    30061 cgacagcaag gtcgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagag
    30121 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc
    30181 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
    30241 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
    30301 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
    30361 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
    30421 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
    30481 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
    30541 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
    30601 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
    30661 cgacttcatc aattacagag ttacgcaggt tattcgccgc gttgccagag aagatcgggt
    30721 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
    30781 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
    30841 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
    30901 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
    30961 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
    31021 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
    31081 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
    31141 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
    31201 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
    31261 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
    31321 cgcgtacatt ttcgttgatt tgtacactga cttcgccgtc gctaaagcga cctacagcgg
    31381 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
    31441 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
    31501 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcgg tgtattaaag
    31561 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
    31621 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
    31681 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
    31741 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
    31801 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
    31861 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
    31921 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
    31981 ccaccggcgt tagtatttca ccaacgcctt cggcaaacga ggcatgcccc cgaacaaaga
    32041 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
    32101 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
    32161 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
    32221 gacgcccgct gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat
    32281 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
    32341 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
    32401 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
    32461 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
    32521 ggtgagttcc atattggctg gcatcgcagg ttgcgttttg gtgtcataac caccataaac
    32581 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
    32641 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
    32701 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
    32761 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
    32821 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
    32881 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
    32941 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
    33001 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
    33061 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
    33121 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
    33181 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
    33241 atcttacgta tcctgtatga tgcaagcaga ctaaccctat caacgttggt attatttccc
    33301 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
    33361 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
    33421 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
    33481 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
    33541 cttaatgaag aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc
    33601 catttaatgc gtgttgccag cggcctagat tcactggttc ttggggagcc gcagatcctc
    33661 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
    33721 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
    33781 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
    33841 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
    33901 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
    33961 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
    34021 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
    34081 aaaggcatgg tggagcgcgc attaaaaagc cgtcgcaacc aaccaatgct gttggtggat
    34141 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
    34201 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
    34261 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
    34321 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
    34381 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
    34441 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
    34501 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
    34561 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
    34621 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
    34681 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
    34741 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
    34801 agatgatgct cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta
    34861 aagaaaaaag cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg
    34921 atgacgaacg taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc
    34981 tgttcgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
    35041 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
    35101 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc
    35161 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
    35221 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
    35281 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
    35341 ttactcactt gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa
    35401 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
    35461 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
    35521 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
    35581 tctaccgcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
    35641 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
    35701 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
    35761 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
    35821 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
    35881 tgaacccatt gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc
    35941 gccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
    36001 gttgcctgaa caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct
    36061 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
    36121 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
    36181 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
    36241 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
    36301 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
    36361 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
    36421 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
    36481 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
    36541 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
    36601 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
    36661 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
    36721 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
    36781 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
    36841 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
    36901 ctggggtaag tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
    36961 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
    37021 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
    37081 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
    37141 ggcgtttatc gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc
    37201 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
    37261 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    37321 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    37381 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    37441 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    37501 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    37561 gaaattcgcg atcgcgggtt gatttatgtg caactggatt gcgaacacgt tgcgttgaac
    37621 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    37681 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    37741 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    37801 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    37861 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    37921 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    37981 aagaagggat gaaaatcttc caggagttga agcagacttt tggcgtgaaa attatcaccg
    38041 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    38101 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    38161 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    38221 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    38281 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    38341 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    38401 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    38461 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    38521 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    38581 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    38641 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta
    38701 cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc cgttttcacc
    38761 ttacttccgg ttacgccacc agccgacaat cgctgcggta ataattcccg ccaggatcgg
    38821 tgctgccagg tcgtgccaga aaatcatggc aaactgcgcg agcgtcatat agccgccttg
    38881 ttgtaatgac aacattttgc ggctattctt gaattgttct ggttcaagat tagcccccgt
    38941 tctgttgtca ggttaaacct ctcaacgtgc gggggttttc tctttccagc aaccaatgcc
    39001 accagggata aagcccccgc aacattgcgc ctcaccggat aacgccggct tgatgtggat
    39061 actacgtctc aattcatctt cacttcatcc ctgaaatgtt tgcaatgaag agtgcattcc
    39121 ggtttttcaa cagctgttac agtcgtttca tgagggctct ggatgatgct tccagctcgg
    39181 gttgccaatg tttactggcg gaagagataa agacaaaaaa ggccgcaggc tgttacccct
    39241 gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt tcgccttact
    39301 tccggttacg ccaccagccg acaatcgctg cggtaataat tcccgccagg atcgatgctg
    39361 ccaggtcgtg ccagaaaatc atggcaaact gcgcgagcgt catatagccg ccttgttgta
    39421 atgacaacat tttgcggcta ttcttgaatt gttgatggtt caagattagc ccccgttc
//
LOCUS       DAZKGS010000024        39180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKGS010000024 DAZKGS010000000
VERSION     DAZKGS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..329)
                     /gene="yfgG"
                     /locus_tag="ACHOQY_001290"
     CDS             complement(138..329)
                     /gene="yfgG"
                     /locus_tag="ACHOQY_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9677429.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            347..499
                     /locus_tag="ACHOQY_001291"
     CDS             347..499
                     /locus_tag="ACHOQY_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677430.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            681..2924
                     /gene="pdeF"
                     /locus_tag="ACHOQY_001292"
     CDS             681..2924
                     /gene="pdeF"
                     /locus_tag="ACHOQY_001292"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9677431.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2963..4504)
                     /gene="ppx"
                     /locus_tag="ACHOQY_001293"
     CDS             complement(2963..4504)
                     /gene="ppx"
                     /locus_tag="ACHOQY_001293"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9677432.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAKRLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGTLREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4509..6575)
                     /gene="ppk1"
                     /locus_tag="ACHOQY_001294"
     CDS             complement(4509..6575)
                     /gene="ppk1"
                     /locus_tag="ACHOQY_001294"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9677433.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6746..7384)
                     /gene="purN"
                     /locus_tag="ACHOQY_001295"
     CDS             complement(6746..7384)
                     /gene="purN"
                     /locus_tag="ACHOQY_001295"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9677434.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7384..8421)
                     /gene="purM"
                     /locus_tag="ACHOQY_001296"
     CDS             complement(7384..8421)
                     /gene="purM"
                     /locus_tag="ACHOQY_001296"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9677435.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8746..9372
                     /gene="upp"
                     /locus_tag="ACHOQY_001297"
     CDS             8746..9372
                     /gene="upp"
                     /locus_tag="ACHOQY_001297"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9677436.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9458..10747
                     /gene="uraA"
                     /locus_tag="ACHOQY_001298"
     CDS             9458..10747
                     /gene="uraA"
                     /locus_tag="ACHOQY_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9677437.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10842..11543
                     /gene="hda"
                     /locus_tag="ACHOQY_001299"
     CDS             10842..11543
                     /gene="hda"
                     /locus_tag="ACHOQY_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9677438.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11681..12040)
                     /gene="arsC"
                     /locus_tag="ACHOQY_001300"
     CDS             complement(11681..12040)
                     /gene="arsC"
                     /locus_tag="ACHOQY_001300"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9677439.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12061..13524)
                     /gene="bepA"
                     /locus_tag="ACHOQY_001301"
     CDS             complement(12061..13524)
                     /gene="bepA"
                     /locus_tag="ACHOQY_001301"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9677440.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13737..14798
                     /gene="yfgO"
                     /locus_tag="ACHOQY_001302"
     CDS             13737..14798
                     /gene="yfgO"
                     /locus_tag="ACHOQY_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9677441.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14836..15684)
                     /gene="focB"
                     /locus_tag="ACHOQY_001303"
     CDS             complement(14836..15684)
                     /gene="focB"
                     /locus_tag="ACHOQY_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP9677442.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15706..17718)
                     /gene="hyfR"
                     /locus_tag="ACHOQY_001304"
     CDS             complement(15706..17718)
                     /gene="hyfR"
                     /locus_tag="ACHOQY_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP9677443.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17748..18161)
                     /gene="hyfJ"
                     /locus_tag="ACHOQY_001305"
     CDS             complement(17748..18161)
                     /gene="hyfJ"
                     /locus_tag="ACHOQY_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP9677444.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18154..18911)
                     /gene="hyfI"
                     /locus_tag="ACHOQY_001306"
                     /pseudo
     CDS             complement(18154..18911)
                     /gene="hyfI"
                     /locus_tag="ACHOQY_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18908..19453)
                     /gene="hyfH"
                     /locus_tag="ACHOQY_001307"
     CDS             complement(18908..19453)
                     /gene="hyfH"
                     /locus_tag="ACHOQY_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP9677445.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19463..21130)
                     /gene="hyfG"
                     /locus_tag="ACHOQY_001308"
     CDS             complement(19463..21130)
                     /gene="hyfG"
                     /locus_tag="ACHOQY_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP9677446.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21120..22700)
                     /gene="hyfF"
                     /locus_tag="ACHOQY_001309"
     CDS             complement(21120..22700)
                     /gene="hyfF"
                     /locus_tag="ACHOQY_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP9677447.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22705..23355)
                     /gene="hyfE"
                     /locus_tag="ACHOQY_001310"
     CDS             complement(22705..23355)
                     /gene="hyfE"
                     /locus_tag="ACHOQY_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP9677448.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23367..24806)
                     /gene="hyfD"
                     /locus_tag="ACHOQY_001311"
     CDS             complement(23367..24806)
                     /gene="hyfD"
                     /locus_tag="ACHOQY_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFP9677449.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24823..25770)
                     /gene="hyfC"
                     /locus_tag="ACHOQY_001312"
     CDS             complement(24823..25770)
                     /gene="hyfC"
                     /locus_tag="ACHOQY_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP9677450.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25781..27799)
                     /gene="hyfB"
                     /locus_tag="ACHOQY_001313"
     CDS             complement(25781..27799)
                     /gene="hyfB"
                     /locus_tag="ACHOQY_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP9677451.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27799..28416)
                     /gene="hyfA"
                     /locus_tag="ACHOQY_001314"
     CDS             complement(27799..28416)
                     /gene="hyfA"
                     /locus_tag="ACHOQY_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP9677452.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28669..29139)
                     /gene="bcp"
                     /locus_tag="ACHOQY_001315"
     CDS             complement(28669..29139)
                     /gene="bcp"
                     /locus_tag="ACHOQY_001315"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9677453.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29139..29711)
                     /gene="gcvR"
                     /locus_tag="ACHOQY_001316"
     CDS             complement(29139..29711)
                     /gene="gcvR"
                     /locus_tag="ACHOQY_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9677454.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29857..30735
                     /gene="dapA"
                     /locus_tag="ACHOQY_001317"
     CDS             29857..30735
                     /gene="dapA"
                     /locus_tag="ACHOQY_001317"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9677455.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30752..31786
                     /gene="bamC"
                     /locus_tag="ACHOQY_001318"
     CDS             30752..31786
                     /gene="bamC"
                     /locus_tag="ACHOQY_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9677456.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31999..32712
                     /gene="purC"
                     /locus_tag="ACHOQY_001319"
     CDS             31999..32712
                     /gene="purC"
                     /locus_tag="ACHOQY_001319"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9677457.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32866..33093
                     /gene="vapB"
                     /locus_tag="ACHOQY_001320"
     CDS             32866..33093
                     /gene="vapB"
                     /locus_tag="ACHOQY_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFP9677458.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33093..33491
                     /locus_tag="ACHOQY_001321"
     CDS             33093..33491
                     /locus_tag="ACHOQY_001321"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP9677459.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33638..34501
                     /gene="ypfJ"
                     /locus_tag="ACHOQY_001322"
     CDS             33638..34501
                     /gene="ypfJ"
                     /locus_tag="ACHOQY_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9677460.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34516..36531
                     /gene="tmcA"
                     /locus_tag="ACHOQY_001323"
     CDS             34516..36531
                     /gene="tmcA"
                     /locus_tag="ACHOQY_001323"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9677461.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36605..37303
                     /gene="ypfH"
                     /locus_tag="ACHOQY_001324"
     CDS             36605..37303
                     /gene="ypfH"
                     /locus_tag="ACHOQY_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9677462.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37413..37613)
                     /gene="ypfN"
                     /locus_tag="ACHOQY_001325"
     CDS             complement(37413..37613)
                     /gene="ypfN"
                     /locus_tag="ACHOQY_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9677463.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37641..38768)
                     /gene="dapE"
                     /locus_tag="ACHOQY_001326"
     CDS             complement(37641..38768)
                     /gene="dapE"
                     /locus_tag="ACHOQY_001326"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9677464.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(38772..39128)
                     /gene="yffB"
                     /locus_tag="ACHOQY_001327"
     CDS             complement(38772..39128)
                     /gene="yffB"
                     /locus_tag="ACHOQY_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9677465.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9588 a        10028 c        10294 g         9270 t
ORIGIN      
        1 aaacaggata aacatcagtc aggacaaaga cgtaaaattt aagatgaaag aatatctgcc
       61 gctttgataa ttccaaagag caaaagcatc cgacagcgaa gtgccgttag gttctgtgaa
      121 agtggagaag gtaaccgcta acgttgtacc ggtgattcga cggagagtgt ggattgctga
      181 gcttcttttt tgctctgatg gtgctgccag gcaccgacgg acgagtagat aaaacggcca
      241 aagaagaaga taaagctgat gagcagtacg atacgggtca tgcgactgtt aaatcggtgt
      301 cgttttcgca tactggttgc ctgactcaca aaaggttcct tgaagtatgt cccacgctgt
      361 ggacggtact tacattaagg cacaacagga caaaatggtc aattcttcgt tatgtaaaaa
      421 agcgtcagtg gatacatatt ttaatgttat ggaagttaat ttaattattt acaatgatga
      481 tgtaataatg atgaattaac atgataaacg taataattca ttaatcaggg ttattttgtt
      541 tgatatatat caattggaac tttcatatga cacttagaat caatgctctc tatagtgatg
      601 aataatcatc attcgaagtc aggtgggatg cctgtctgaa tacacctcct tcaggatgtg
      661 ggggattctg ctgagcatct atgaaactga atgcaactta tataaaaata cgtgataaat
      721 ggtgggggct tccgctgttc ctgccttctt taatcttgcc cattttcgcc cacattaata
      781 ctttcgcgca tatttcttcc ggtgaggttt ttctctttta tctgcctttg gcactgatga
      841 tcagcatgat gatgtttttc agctgggcgg cattgccagg gatcacctta gggatttttg
      901 tccgcaaata tgcagagctg ggtttttacg aaacgctctc attaacggct aattttatta
      961 tcattatcat tctctgttgg ggcggttaca gggtctttac tccccggcgt aacaacgttt
     1021 cacatggtga tacccgttta atttcccagc gtatattctg gcagattgtg tttcctgcaa
     1081 cgctgtttct gatacttttc cagtttgctg catttgtagg attactggcg agcagagaaa
     1141 atctggtcgg tgtcatgcct tttaacctcg ggactttaat caattatcag gccttactgg
     1201 tgggtaatct gatcggtgtc ccgctgtgct acttcatcat tcgggtagtg cgaaatccat
     1261 tttatttacg tagctattat tcgcaattaa aacagcaggt tgatgccaaa gtcaccaaaa
     1321 aagagttcgc gctctggcta ctggcattag gtgctttatt gttgctgtta tgcatgccgt
     1381 taaatgaaaa aagcacaatt tttagcacca attatacctt gtcattattg ctgcccctga
     1441 tgatgtgggg agcgatgcgc tatggttata agctgatttc gctgctctgg gcggtcgtgt
     1501 tgatgatcag catccacagc tatcaaaatt acattcccat ttatcctggc tataccacgc
     1561 agctgaccat aacctcctcc agttatctgg tattctcttt tattgtcaat tatatggctg
     1621 tactggcaac tcgtcagcga gcggtagtca gacgcattca gcggcttgcg tatgtggacc
     1681 cggtggttca tctgccaaat gttcgcgccc tgaatcgcgc gttgcgtgat gccccctggt
     1741 ctgcgctttg ttatttacgc atccctggca tggaaatgct ggttaagaac tatggcatca
     1801 tgctgcggat tcaatacaag caaaaacttt ctcactggct gtcacccttg ctggaaccgg
     1861 gtgaagatgt ttatcagctt tcgggtaacg atctcgcgct gcggctgaat acagaatcgc
     1921 accaggagcg cattaccgca ctggatagcc atctcaagca atttcgtttc ttttgggatg
     1981 gcatgccgat gcaaccgcag attggcgtca gttactgcta tgtgcgctcg ccagtgaatc
     2041 atatctacct gctgctggga gagctaaata cggtcgccga actttccatc gtgaccaacg
     2101 ccccggaaaa tatgcagcgt cgcggggcaa tgtatttgca acgcgaattg aaagataaag
     2161 tcgcgatgat gaatcgacta cagcaggcgc tggaacacaa ccattttttc ctgatggccc
     2221 agccgattac cggtatgcgt ggtgatgttt accatgaaat tcttctgcgc atgaaaggtg
     2281 agaatgatga actgatcggc cccgatagct tcttgccggt cgcgcacgaa tttggtttat
     2341 cgtcgagtat cgacatgtgg gtcattgagc atacgctgca atttatggct gaaaacagag
     2401 cgaagatgcc cgctcaccgt tttgctatta atctgtctcc aacctcggta tgtcaggctc
     2461 gttttcctgt tgaagtcagt cagttgctgg ataaatatca gattgaagcg tggcaactta
     2521 tttttgaagt caccgaaagt aatgctctga ccaatgttaa gcaggcgcaa atcaccttgc
     2581 agcatcttca ggaattaggc tgccagattg cgattgatga tttcggcacc ggctacgcca
     2641 gctatgcgcg gcttaaaaat gtgaatgccg atctgcttaa aattgacggc agttttatcc
     2701 gcaatattgt gtcaaatagt ctggattatc agatagtggc atcgatttgc cacctggcgc
     2761 gaatgaagaa aatgcgggta gtggcagagt acgttgaaaa cgaagagatc cgcgaggcgg
     2821 tgctctcttt ggggatcgat tatatgcagg gttatcttat tggtaagccg caaccgttaa
     2881 ttgatacgct gaatgaaatc gaacccattc gcgaaagtgc ctgaataatg cgggccgaca
     2941 tttctcgtcg gcccgcaaag cattaagcgg cgatttcagg tgtactttct tcttcaattt
     3001 tcaaccgcca gccagccacg ccttcccagt attcttgctc cttttccaga tcaagcagta
     3061 ccagcgcatt ctgactaaac cagtcatgcg ggaaacgcag tgtccagtga ctgtcatcag
     3121 taatcagtgt caatgttggc ggtgtggtgg ttgcctgacg ttgattgttt agtaatacgc
     3181 caaggcgcaa tagctgtatc agtggcagga actgtttctt cttaaacaag gtaaagcgcg
     3241 gcagatcgtc gagcttaatc gctttacggt gatagcgcac cagtgtcgcc atcatcagct
     3301 gctgttcctg attaaaaccc ggcaagtcac tgttttgcag aatataagcg gagtggcgat
     3361 gcaaaccgct gtggttgata ttcaacccga cctcatgcag catggcggcc catcgcagta
     3421 gcgcctccag ttgcggatgc gccagcttcg gttgctgttc ccgccactgt tcgtacattt
     3481 gcatagtggt atccagcact cgtcgggcct gttcgctgtc gatgtgatac tggttggcga
     3541 ggctgctggc ggtgcgacta cgcacatcct gatgacggaa acgtccttcc atttcataca
     3601 gtacgccttc gcgtaacgtc ccgtcagaaa ggcgcagttc acggatggct aaagcatcaa
     3661 acacaccaca taaaatcgcc agtccaggaa cgaagactgt tttccgctct tcggaaagac
     3721 ccggtaaact cagcgatgcg aaattacggt gacgtaaaac ttcttttacc agtttttcca
     3781 gacgttccgg ggtaattatc ccgtctttct cgcccatttc cattaatact tcatgggcgg
     3841 cttttatggt accggaagcg cccattgcca cgttccagcc ctgaatacgg aattgccagg
     3901 ttaaagtttc cagtttttgt gctgccgcca tgcgagcgcg ctgaaaattc tctttattga
     3961 tgaccccgcc agggaaataa agctgggcaa agctgacaca acccatccgg cggctttcaa
     4021 cgagaatagg ttcgaaattt tcaccaatca ccagttccgt agatccgccg ccaatatcaa
     4081 taaccagttt gcgacctttt tccggttggg tatgttccac gcccataaaa atcagacggg
     4141 cttcttcatt accggaaata atttcaatcg ggtagggaat gaccttttcc gcgcgtttca
     4201 gaaagtcagt ggcgttcagc gcctgacgca gtgtatgggt gccaactata cagacgctgg
     4261 caggagaaaa cccttgtagc cgtttggcaa acagcgacag acagttcaaa ccgcgcgtca
     4321 ttgcctcttc actcaacata ttatctggcc ccaggccgtc cgccagatgc acccgctgtt
     4381 tcaggcggcc aataatctgc atggcaccat ctaccacacg ggctatgacc atgtgaaaac
     4441 tgtttgaacc aagatcgacc gcagcaaact cctgcggacg aggggattta tcgtgtattg
     4501 gcatagggtt attcaggttg ttcgagtgat ttgatgtagt cataaatcgc caactgcgcc
     4561 cgtactttgc ggcgattgcc gcggggaacg tagcgattac tgagttcttt atcgatataa
     4621 cgtgctttga ccgtatcgct gaacaatatg tcgatgatgt ccagcacccg ctgcttcagg
     4681 cgcggatcga gcagcggtgt cgccacttca atacgataat caatattgcg cgtcatccag
     4741 tcggcggaag aaaggtagac ctttttatcg ccgccatttt caaaaatata aacccggtca
     4801 tgttcaaggt aacggtcaac aatactgatg gcacgaatgt tgtcgctaat gccttccaga
     4861 ttggggatca gcgaacacat tccgcgaacc agcagattaa ccggtacgcc ggagctggag
     4921 gccgcataca gacgatcaac caggccttta tcgacaaggt tatttagctt cagggtgata
     4981 ccactgggca gcccttgctg cacgttggcg atttcgcggt ctaccatttc atataacaga
     5041 cggcgagagt tttgcggtga caccattaaa taatcaaatg tcactgggcg gtatgggttt
     5101 tcaataaagt taaatacccg ccgtacttcg ttggtgatac gcgcatctgc ggtcagcaac
     5161 gaatagtcag tataaagacg cgcggttttt tcgttaaagt tcccggtccc gatgtgtgcg
     5221 taacggacca cttcaccgtt ttctttacgt gaaatcagga acagtttggc gtgaattttc
     5281 agccccggcg cagagaagat aacgtgcacg cctgcttcgg tcaggcgctt cgcccagtga
     5341 atgttggctt cttcgtcgaa acgcgcctgt aactcaacca ccacagtgac tttcttaccg
     5401 ttatgtgcgg cgtggatcat cgagtcgatg atgcgtgaat ctttcgccac gcggtaaatg
     5461 ttaattttaa tcgccagtac gctcgggtcg aacgaagcct gacgcagcag ttccagcaca
     5521 tgctcaaagg tgtgataagg ataatagagc aacacatcgc gttcgcgaat ggcatcaaaa
     5581 ccattgcgga actgggcttt atcaaaccaa atgtggcgta aacgcggcag tggtttgttc
     5641 accagattgg ctttgccgac attggggaaa ttaataaagt ctttaaaatt atgataacga
     5701 ccgccgggga cgatggagtc gtagcgggaa atagtcagtt tttcgcgtaa cacttcaacc
     5761 agcgcattgg gcatatcgcg ctgataaaca aaacgcaccg gctcagcagt taaacgctgc
     5821 ttgagactgg aagacatcaa ctccatcagg ctggcttcca tctcatgcac taaatcgtat
     5881 tcggcatcgc gtgtcatctt cattgaatag gcattcagcg cgtcataatc aaagaagcct
     5941 ttgaaaatat catcaaggca gtaacgcaga atgttatcca gaagaatcat cggcttgcgt
     6001 cgacgcggcg cttctggcgg taaattcaca aagcgcggca ctttatctga tgggatctcc
     6061 agcagcgcgt aacggatggt atcaccacgg ataatttcca ccgccagata ggtgtaatca
     6121 tccttcagga actgcactaa gtcagtgtca ggattgatta aaatcggcgt aatgtgctga
     6181 cgcagatact gcttaaaata atgacgaagc cagttttgtt gattgacgga gagctggcgt
     6241 tcattaatca ggaagatctg gttgcgcgcc atctccagca atagctcgtt gtagaggccg
     6301 tcgaattcct gatcggcttt cagcacccgg gactgaattt tgcccagtaa atggcgggaa
     6361 tgagagttgg agccttgttc ttcgctaata atgatgcgtc gcttcagttc agcgaagcgg
     6421 actttataga actcatcaag gttattggaa tagatcccca ggaaacgcat cctttcaatc
     6481 agcgggttag atttgtccgc cgcttcctga agcacgcgtt cattgaacga taaccaactg
     6541 agctcttttt cgatgtatag cttttcctga cccattacca cttttactcc gttttattca
     6601 cgggacactg cctggatatt atggcgagca atattggcag atgtccaact atgccagaaa
     6661 agggggaaaa aaactgctgg agcttgcgat ttacaggtga attgaaaaac gccagcggca
     6721 gagctggcgc tttaactacg gggcattact cgtcggcagc gtagccctgc ggcggcagac
     6781 gttgaccatc cagccacgcg gcgttttcgt gcattttcag acgaccatcg gcaaaccagc
     6841 taatcaccag tggataaata gcgtgttcct gggtttgcac gcgggcggtg atgtcatctt
     6901 ccgtatcacc agcaaatacc gggactttcg cctgtaaaat aaccgggcca ccgtccagtt
     6961 catcggtgac gaaatgcacc gatgtaccgt gctcttcatc accattttcc agcgcttgac
     7021 gatgggtgtg taatccggga tatttcggca gcagagaagg gtgaatgttc agcaaacgcc
     7081 cggcatagtg ggagacaaac gccgggctga gaatgcgcat aaaaccagcc agcacgacca
     7141 catcgggtgc gtacatgtcg atttcatgaa tcaactcccg gtcataggct tcacgactgt
     7201 caaacgcgct ggcgatgagc gtatgcgttg caatacccgc ctggcgggcg cgttcaaggc
     7261 cgaacgcgtc ggccttattg ctgaaaactg cccgtacggt gcctttaatt ttgttggttt
     7321 tacaggcgtc aataattgcc tgtaaattac ttccgttacc ggaaataagc accacaatat
     7381 tcattattcg ataaccacgc gttgttcgga atcagaggct ttgatgatac cgattttcca
     7441 cgcgttttca ccgttggcat tgagcagggc gagggctttg tccacttccg gagcaggcag
     7501 ggcaataatc atcccgacgc cgcagttgaa ggtgcgatac atttcatggc gctcaacgtt
     7561 acctgccgtt tgcagccagt tgaacacttc cggccactgc caggaagatt catcaatcac
     7621 tgcctgagta ttatctggca atacgcgtgg aatgttttcc cagaagccgc cgccggtcag
     7681 gtgcgcaatg gcatgcacat cgaccttttc aatcaactcc agcactgact tcacgtaaat
     7741 gcgggtcggt gccagcagat aatcggctaa tggcttacca tcaagttggg tggtttgcgg
     7801 atcacaaccg ctgacttcaa gaattttgcg caccagcgaa tagccgttcg agtgcggacc
     7861 gctggaaccg agtgcaatca gcacatcgcc gtcgctgact ttagagccgt cgatgatttc
     7921 tgatttttct accacgccaa cgcagaaacc cgcgacatcg taatcctcac cgtgatacat
     7981 ccccggcatt tctgccgttt cgccacccac cagtgaacag cccgattgca gacatccttc
     8041 cgcgataccg ctgatcaccg ctgaagcggt atcaacatcc agttttccgg ttgcgtaata
     8101 gtcgaggaaa aacagcggct ctgcaccttg caccaccagg tcattaacgc acatggcgac
     8161 cagatcaata ccaatggtgt cgtgacgttt taagtccatt gccagacgca gcttggtacc
     8221 tacgccgtca gtgccggaaa ccagcacggg ttcacgatat ttttgcggca atgcacacag
     8281 cgcaccgaag ccgcccagac cgcccatcac ttccggacga cgcgttttct tcactacgcc
     8341 tttgattctt ccaaccagag cattacccgc gtcaatatca acaccggcat ctttgtagct
     8401 aagagaggtt ttatcggtca ctgcttgggt ccccacgcgt tacttgcggt agaaaaataa
     8461 aattcggcgc aattctaaca gggaaagcaa acgtttgcga gactgcttta cacaaccttt
     8521 ttgcacgtct tttgcccagg cgcgcggcga aagaagactt gtaccagggt aaaggttagt
     8581 tttcggatgg aataatcttc tttcataacc atttgaatat aaaataactt tatctcaagc
     8641 cgttatcatt ttgactaaag tcaacgaaaa gaatattgcc gccttgaaga aaggaggtat
     8701 aatccgtcga ttttttttgt ggctgcccct caaaggagaa agagtatgaa gatcgtggaa
     8761 gtcaaacacc cactcgtcaa acacaagctg ggactgatgc gtgagcaaga tatcagcacc
     8821 aagcgctttc gcgaactcgc ttccgaagtg ggtagcctgc tgacttacga agcgaccgcc
     8881 gacctcgaaa cggaaaaagt aactatcgaa ggctggaacg gcccggtaga aatcgaccag
     8941 atcaaaggta agaaaattac cgttgtgcca attctgcgtg ccggtctggg gatgatggac
     9001 ggtgtgctgg aaaatgttcc gagcgcgcgc atcagcgttg tcggtatgta ccgtaatgaa
     9061 gaaacgctgg agccggtacc gtacttccag aaactggttt ctaacatcga tgagcgtatg
     9121 gcgctgatcg ttgacccaat gctggcaacc ggtggttccg ttatcgcgac catcgacctg
     9181 ctgaaaaaag cgggctgcag cagcatcaaa gttctggtgc tggtagctgc gccagaaggt
     9241 atcgctgcgc tggaaaaagc gcacccggac gtcgaactgt ataccgcatc gattgatcag
     9301 ggactgaacg agcacggata cattattccg ggcctcggcg atgccggtga caaaatcttt
     9361 ggtacgaaat aaagaataaa aataattaaa gccgacttta agagtcggct tttttttgag
     9421 taaagcgcct ataacacata atacagagga taatactatg acgcgccgtg ctatcggggt
     9481 gagtgaaaga ccgccacttt tacagacaat cccgcttagt ttgcaacatt tgttcgccat
     9541 gtttggtgca accgtcctgg tgcccgtctt atttcatatt aacccggcga ctgtactgtt
     9601 atttaacggt attggaacgc tgctgtatct cttcatctgt aaagggaaaa ttccggctta
     9661 tcttggttcc agctttgcct ttatttcacc ggtattgtta ctgttgccgt tagggtatga
     9721 agtcgcgctg ggcggcttta ttatgtgcgg cgtgctgttc tgcctggttt cttttatcgt
     9781 gaagaaagcg gggaccggct ggctggacgt gctgtttcca cctgcggcaa tgggcgcaat
     9841 cgttgccgtc atcggtctgg agctggcggg cgtagctgcc ggtatggcgg gtttactccc
     9901 ggctgaaggg caaacgccag actccaaaac catcatcatc tctattacca ccctggcggt
     9961 cacggtttta ggttccgtgc tgtttcgtgg tttcctggca attatcccga ttttaattgg
    10021 cgtgctggtg gggtacgcgc tctctttcgc aatgggaatt gtcgatacca cgccgattat
    10081 taatgctcac tggtttgcgc tgccaaccct ctatacgccg cgcttcgagt ggtttgccat
    10141 tctgactatt ctgccagcgg cgttagtggt tattgccgaa cacgtagggc acctggtagt
    10201 aacggctaat atcgtcaaaa aagatctgct gcgcgatcca ggtctgcacc gttcgatgtt
    10261 tgctaatggc ttgtcgaccg tgatttccgg cttctttggc tctacgccaa ataccactta
    10321 cggagaaaac attggcgtga tggcgatcac ccgtgtttac agtacctggg ttatcggcgg
    10381 ggcggcgatt ttcgctatcc tgctttcctg cgtcggtaaa ctggctgccg ctatccagat
    10441 gatcccattg ccggtgatgg gcggcgtttc gctgctgctt tatggtgtca tcggtgcttc
    10501 cggtattcgt gttttgatcg aatcgaaagt ggattacaac aaagcacaga acctgatcct
    10561 gacttccgtg attttgatca tcggcgtcag tggcgcgaag gtaaacatcg gcgcggcgga
    10621 gttaaaaggt atggcgctgg cgaccatcgt cggtatcggc ttaagtttga tcttcaaact
    10681 gatcagtgtg ctgcgccctg aagaagtggt gctggacgca gaagacgcgg atataacaga
    10741 caaataatgt accgaccggg cagtgttgct gcccggttca aacatcatgg gattctgtgg
    10801 taaacttctc gcgattttgt gaaatcctgg ttgaggtatc tctgaacaca ccggcacagc
    10861 tctctttgcc actttatctt cctgacgacg aaacctttgc aagtttctgg ccgggggata
    10921 actcctcttt actggctgcg ctgcaaaacg tgctgcgtca ggaacatagc ggttacatct
    10981 atctctgggc acgcgaaggc gcggggcgca gccatctgct gcacgcggct tgcgcggaat
    11041 tgtcgcagcg tggcgatgcg gtgggctatg tcccgctgga taaacgcacc tggtttgttc
    11101 cggaagtgct cgacggtatg gagcatttgt cgctggtctg tatcgacaac attgagtgta
    11161 ttgcaggcga tgagttgtgg gagatggcga ttttcgatct ctacaatcga attctggaat
    11221 cgggcaaaac acgattgttg atcaccggcg atcgtccacc gcggcagttg aatctgggat
    11281 taccggatct cgcgtcgcga ctcgactggg ggcagatcta caaattgcag ccactttctg
    11341 atgaagataa gttgcaggcg ctacagttac gcgcgcgttt gcgtggtttt gaactgccgg
    11401 aagatgtggg gcgtttcttg ctgaagcggc tcgacagaga aatgcgcacg ctatttatga
    11461 cgttggatca gttggatcgt gcgtcgatta ccgcgcaacg taagctgacc attccgtttg
    11521 tgaaagaaat tctgaagttg tagataaggt gtttattgtc ggatgcggcg cgaacgcctt
    11581 atccgaacta cgcgaagtta atccatagta ttcgtaggcc ggataagacg tggcaagcgt
    11641 cgcatccggc aatgtgcttc aactagcgat ggagcacatc ttaacccacg atttccagta
    11701 cctgctccgg tggacgacca atccgcgctt tgccattcgc gaccacaatc gggcgctcca
    11761 ttagcttcgg attatccacc attgcctgaa tcagcgcctc ttcgctaagc gaactgtcag
    11821 ccaggttcag ttctttataa agatcctctt tctggcgcat caattcacgg gcgctattca
    11881 tccccagcat tttcagcaaa tcgcgcagcg ttgccgcatc ggcgggtgtc tcaaggtaga
    11941 gtaccacttc tggctccacg ccgttttctt tcagcaggtt cagcgtttcc cggctctttg
    12001 aacagcgtgg gttatggtag atttttacct gtttggtcat tgttcttcct ttaatgcgaa
    12061 ttacatcttg gtataaggct taaagcgttc ctgcagctgg cgcaactggt cgatgcgcgc
    12121 atcgtaacgc gcttgttgca ggctgcctaa tttcacctgc gaactggcgc tactcaacag
    12181 cgaaatggcc tgatcgagtc gtccggcgag cgcataacct tctgctcgcg cagccagctc
    12241 ctgatcgcgg ttatttagcg cggcttccgc ctgtgccagt aaatcccagc cgttgctgtc
    12301 atctttatta ttaaaggtgt agcgattcag aatattggcc gcttcttgtg gttgaccgcc
    12361 ttgtagataa gcgttcgcca ggttgagctg caacaccgga ttggtgcgca aatcacgggc
    12421 atttttcagg cgattgattg cctcattggc tttgttttgc ccaagatcga tatcggtagc
    12481 cagatcgaga taccatgcgt tgccaggttc tgccgccagt aacggttgca gcgttttacg
    12541 cgcctcgtcg tatttattgg cttccatcgc ctgtaaagca cgaccatatt gcgccgctcg
    12601 ttgctgacga acgtttcctt tcgcccattc atccagcaaa tcactggtga gctggttacg
    12661 tccggaatta tacatcccca gtgtgcgcgc tttcgccaga tagaaatctt ccgacgactg
    12721 caccaccatc ggtcgcatct gattagcacg gttgcgggca tctgccagac gactttccgg
    12781 caacgggtga gtcaataaaa tttccggcgg gcgcgaggag taacgcgcct gatcgagtaa
    12841 tttttcgagg aaggttggca tcgcctgcgg atcgaatccc gagcgttgca gcacctgaat
    12901 accaatgcgg tccgcttcct gttcattttg ctgggtgaaa ctgatcattc cctgacgtgt
    12961 tcccgccagt gtaccggtca gcgccgccat ccccgcctgc ggactggcca tcgccagtaa
    13021 aatagaacct aacgcgccga cccaggtcag cggcgcgctg cgttgttgat cttccatcgc
    13081 tcgcgccagg tgacgttggg tgacgtggga gatttcgtgc gccataactg aagccagttg
    13141 actttcgtta tcggaataac ggaacagggc agagtgcagc accacgttgc cgccaaagaa
    13201 agcaaaggcg ttaatttcgt cgttgttgat cagaaaaaaa tgaaacggtg tcttaaccga
    13261 attggcatgc gaaaccagac gcatccccag cgaattaata tattgcgtta acagcgggtc
    13321 attaattaac ggcgcgctgc cgcgtagctg gcggacataa tagtcgccca tctgcatttc
    13381 ctgaccaatg gaaagcgtgc ttcctgcgga ggttcccata tccggcaagg tgtctgcgct
    13441 gtcggcaaac gccggggcta cctgaccaat ggtcatagca gcaatgaggg ttgcaaccag
    13501 gttttttttc aactgcctga acataacctc tatcctgtat ttctgaagat agtcatttga
    13561 ccgatactgt taaatatcgt tcccaccgcg ccgcctttga gtgtaacggc gaagattagc
    13621 ccactcaaat gacttttcag tttcctgaat ccgtcataaa aagcgaagtc ttttttgtga
    13681 catttcgata caattgcgga tcgcaatccc gctcttgagg ttcagggaag gtttttatgc
    13741 tcgaaatgtt gatgcaatgg tatcgccgcc gttttagcga cccggaagcg attgccttgc
    13801 tggttatttt agttgccgga tttggcatta tctttttctt tagtggcctg cttgctccgt
    13861 tgctggtggc tattgtgctg gcctatttgc tggaatggcc aaccgtgcgc ctgcaatcta
    13921 ttggctgctc ccgccgctgg gcgacgtcga ttgtattggt ggtttttgtc ggtatattgc
    13981 tactgatggc gttcgtggta ctgcctatcg cctggcaaca gggcatctac ttaatccgcg
    14041 atatgccggg gatgctcaat aagctttctg actttgccgc cacgttgccg cgccgctatc
    14101 cggcgttaat ggacgcgggc attattgatg caatggccga aaatatgcgc agtcggatgc
    14161 tgaccatggg cgattcggtg gtgaaaattt ccctcgcctc gctggtcggt ttgctgacca
    14221 ttgccgtcta tctggtgctg gtgccattga tggtcttctt cctgctgaaa gacaaagagc
    14281 agatgctgaa cgccgttcgt cgggtgctgc cgcgtaaccg tgggctggca ggacaggtgt
    14341 ggaaggagat gaatcaacaa atcaccaact atatccgcgg caaagtgctg gagatgatcg
    14401 tggtagggat cgccacctgg ctggggttct tgctcttcgg gctgaactat tcgctgctgc
    14461 tggcggtgct ggtcggcttc tcggttctta ttccgtacat tggcgcattt gtggtgacca
    14521 ttccggtggt tggcgtggcg ctattccagt ttggcgcagg cacggaattc tggagctgct
    14581 tcgcggtgta tctgattatt caggcgctgg acggcaacct gctagtaccg gtgttgttct
    14641 ccgaagcggt taacctgcat ccgctggtga ttattttatc ggtggtgatc ttcggtggtt
    14701 tgtggggatt ctggggcgta ttcttcgcca ttccattggc gacgctgatc aaagccgtga
    14761 tccacgcctg gcccgatggg caaatcgcgc aagaataatc ttcagcccga cgaactgttt
    14821 cgccgggctg atttttcagg attcctgacg taaataaata gcccgataac acatactcac
    14881 cagcactgca ccgccgataa tattacccag catcaccggg agcagattgg cggtaataaa
    14941 atggctgacc gtcagtgccg gaaaattgtc tgcgctactg tgcgccagtt gccagaagga
    15001 ggtaggggcg aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca
    15061 gtgctcaaag ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc
    15121 gcataacgaa cgcccgcaat aactcatcca cagcgccagg caaaccatca gattgcacat
    15181 aatgccgagg ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa
    15241 aaccgcgact ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa
    15301 actgaaacag gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca
    15361 agttcgccaa ctaataacgc ccctactttt tgccattacc gtcattaccg acgaggtgaa
    15421 caggttggtg ccgcaaaccg ccagcaaaat aaagccaagt gtaaagcaaa ggccgcccac
    15481 cagatgggtt aatgcctgtg aagacggggc atcggcgata actgaaaggt aaaaagtaaa
    15541 tccgatcgcc ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttactgcg
    15601 ggcaatttta tgggcggcaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa
    15661 gtcgaaagag agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg
    15721 aaccgagatt cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg
    15781 tgtcgccgcg ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat
    15841 gcgctgacgt tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga
    15901 actgcgaagc gctgaatctt catttagcgt cggcagtaaa cggctttgtc ggacattaag
    15961 atgtaaattc agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag
    16021 ctcgcgcacg ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat
    16081 ggcgtcaatt gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag
    16141 agggatatct tccggacggt cgcgcagcgg cggcaattcc agtgggaaga cattcaggcg
    16201 ataaaagaga tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt
    16261 ggcggcaatg actctgacat ttaccgggat cgttctactc ccgccgagac gctcaatctc
    16321 ccgttcctgc aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc
    16381 atcgagaaac aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc
    16441 accagtaaac gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat
    16501 ggcagcgcag ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc
    16561 tctggcgatc acctctttgc cggttccggt ttcaccgcaa atcaacaccg tactgtcgct
    16621 cttcgccaca atatctacct gctggagtag gtcttccatt gcctggcttt gatagataat
    16681 gtcaccgatg cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa
    16741 actttcctgc aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat
    16801 gcgatcggct atgtgttgta gtagctggca gttttcctca ctaaagagag tggaagaggt
    16861 atgcgccagc aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc
    16921 agattcgcag ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag
    16981 atcgtctgca cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac
    17041 actgtcgcca ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca
    17101 ccacaggcta aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag
    17161 accgaaaaaa tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg
    17221 tgacagcact gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag
    17281 caaacgcagc gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat
    17341 tatccgcgcc agccagttaa acagtgtgca atcctgggga gtaaaacggc tgccgtgggt
    17401 acgaataaag cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata
    17461 atcgaccatc tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta
    17521 gcggtaatag aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg
    17581 cggcggcatc gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gtgctaaccg
    17641 ctccgcgagc attccgttta ccgcttcaat tgttattcct tgtggcgggg caaacatcgc
    17701 ctcgtctgac atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt
    17761 ctggccatca gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga
    17821 atgctacgcg cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc
    17881 aacagggcgg tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc
    17941 ttacgtcggg cttgctcctc ttcgacctgg gcaagccaat cttcgtattc cgtcagtggg
    18001 caggggaagg cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag
    18061 taatacatta cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt
    18121 cgcagcgtcc agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg
    18181 aaagagttgc tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt
    18241 aacccgattt cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg
    18301 cccctgacga tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg
    18361 cgggatctgc ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc
    18421 gtgaatcttc tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg
    18481 tgttggcggg cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca
    18541 gacgctgtag agatcgtgga aaatccgccg ccggcaccgc acgcgccgta ggaaacacag
    18601 attttatgat cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg
    18661 actgcgcctg taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca
    18721 aaacgttctg cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg
    18781 cagccgccgc aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc
    18841 tgcttcatct tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc
    18901 actggactca tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg
    18961 tcgcgcgttg tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt
    19021 gtggcgcatt ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg
    19081 caaacgggcg ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat
    19141 cggctttatt ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc
    19201 acacttcttc acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg
    19261 aattttgctg gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgcagg
    19321 caccgcaggc aatacattgg ctgggcatca ggtccggttt cccgcgaaag cctgggctga
    19381 cctccagtgg cgcgaaggga tatttcaccg tcgcggttcc ggcgcgcata atagttttca
    19441 gtaacttcag catcttctgt ccttacttca gcggcgaacg gttacgatca ataccgtagc
    19501 gttcgatctc tttatacggc acggtttttg actggcgctt gcgcacatct accagcgtca
    19561 cacggtcggt acaggagtag cagggatcaa ggctaccgat aatcagcggt gcgtcagaaa
    19621 cggtattgcc gcgcagcatg taacgcaaca ccggccagtt ggcgtaggtg gcggcacggc
    19681 agcgccagcg gaacaatttt tggttatcac cgagcatgct ccagtgcacg tcttcaccgc
    19741 gtggcgcttc aacaaagccc agcgcgaatg cgtgaggttt atagctaaag ccttcggtca
    19801 gcagtggggt atccggcatg ttgtcgaggg caaattccag cattgccagc gaatcaaacg
    19861 tctctttgac acggaccatc acgcgggaga aaacatcgcc gccggtaaag gtaaacagtg
    19921 tttttggaat attgccgtag tcggcgtagg ggtgatcaaa gcgcaaatca cgggcgattt
    19981 gtcggtcgag aatgccaatg ccctgagtgc gttgttccat attcggcgta gcaagcagca
    20041 tctctaccag ctccgacacg tcggcgcgca tctcgcgcac cagtttcagc gtttgcagac
    20101 gttgctcttt gagaatatcg cggcgaacac caccaatcag attcagaccg taggttttac
    20161 gcgacccgat cagcaattcc gccatcgtca tcgacttttc ccgcacgcgg aaaaattgca
    20221 taaagccggt atcaaaacca acgaaatggc aggagaggcc aaggttaagc aaatgactgt
    20281 gtagccgttc gacttccagc agaatcgagc gaatagtatg tgctcgttgc ggcacctcaa
    20341 tccccagtgc attttcaacc gagttggtat aggccacact gtgggcaaaa ccgcaaatcc
    20401 cacacacgcg gtccgataag aaggtcactt cgttgtagcc catccgcgtt tctgccagtt
    20461 tctccatgcc gcgatggaca taaaacaggc ggtaatcagc atcgacaatt tgctcgccat
    20521 ccacaaacaa gcggaagtga cccagttcat cggaggtgat atgcagcggg ccgacaggga
    20581 tcacctgcgc atcgctgttg ccctcattga taaacggata cgtttcggaa tcagtcgtcg
    20641 gttcagggcg cagtcgataa tccatcgcat ctttgcgcag cggatgcata tcttccggcc
    20701 agtcatcggg caacaccagg cgacgatgat ccggcaggcc aaccggaatc agcccgtaca
    20761 tatcgcgaat ttctcgctcg ccccagaccg cggcagggac gcgcggtgtg actgacggaa
    20821 actcccgact gtcggcatcg accagtgcct tcaccacgat ccagcatttt tcggcccctt
    20881 ccattgaaag ggcataataa accgcgtaat gaccgttaag tgtccgctcg tcgttgccaa
    20941 acaggactgg aagccagcca tcatgttgat aataaagata atgtacaacg tcaggcagca
    21001 gattgatttt caccgtaatg gtgacctgtt caggcgtttg tcgctcttca tccagcaccg
    21061 cgccggggaa ctgcgttttc agggcggcga gaatcgcttc gccacgattt gacgatgaat
    21121 taacgttcac tgtgtttctc cgaaacagat gcggtgcctg aaggcaaaaa atcatgccag
    21181 gtgctacgtt gtgcaggcag gtcgtgcgtc cctgagagga ctatagtgga agcgcccgcc
    21241 aggatcctga tgacaggttg tggaatatgc gttcccatcg ccagcatcat gaccagcaga
    21301 atcaccattg gcgaggtggt caaccagccg agatcacccc ggttaacggc ctgcggcggt
    21361 ttcgccatta acacccgcgc tgccatccgt accaggcccg ccagcaccag cgttaacagc
    21421 aataacagca ggacgataat cagcaggtga ttacgtgcca gtccggcggt aatggtcata
    21481 aattcgctaa gaaaaatgtt gaagggcggc atccctgcca gcgccagcgc accgccgcca
    21541 aacagcacgg cggtaaatgg catgattttg agcatcccac agacgacgtt gagatcgcgc
    21601 gtgccgtact tgagcagtac attgccggaa ccgcagaaca gcagcgtttt tgccagactg
    21661 tggtttaagg tgtgcagcag cgcggcaaaa attcccagtg gcccgccaat gcctagcgcc
    21721 accgcgacca gtcccatgtt ctccacgctg gagtacgcca gcagacgctt aatgtcccgc
    21781 tgtaccagaa tgaaaaatgc cgccacggca accgacaaca tgccgaagat gagcaacaac
    21841 cggttgggga aatcgctgcc gatggcctgg cagataatga tgtaatagcg aattagcacc
    21901 aacagcgcgc agttcagcaa tacggcggag agcagggcgc tgaccggact cggtgcttca
    21961 ctgtgagcat ccggcagcca ggcgtgcatg ggaaatagcc cggttttggt gccaaagcca
    22021 attagcacaa acacaaaggc caacagcatt aatgttgggt caagcaagga cgattgctta
    22081 agaacctcgc tccagaatat cgccatttct gcctgcggca taacgctggc gacgttggcg
    22141 tataccagca cggtaccgaa cagaccaaaa gcgacaccaa cagtacaaat aatgatgtac
    22201 ttccatgctg cttccagcga tgaacgctga ccgtaaatgc ctaccagaaa cgccgagctt
    22261 aaggtggtgg cttcgatcgc cgcccacatc acaatcaggt tattgctggt aacaaccagc
    22321 agcatggtaa acaaaaacag atggaagaaa ccgtagtaat cgcacagcgt aacgggggaa
    22381 agctcgccgt gtgccacttc atgacgcatg tagccaatcg agtaaacacc ggtgagaaag
    22441 ccaatcacac caagaatggc gaggaacaaa ccgcccagac catcaatatg aagccacagt
    22501 cccgcagcga atatttctcc tgcatcagcg gcagtttgga ccacccagag tgccagaatc
    22561 agcagcagtg tgatccctaa gctatgtaat acagtcaccg tacaagtcgc agagagcccc
    22621 cgtttccggc aggcaaaaca gagcagcgaa aaaagcagcg gcgtgagcag gagtaaagcg
    22681 aacatcacag aataactcat ctcattatcc cttcagcgcg gtcaagttgt tcacgtccag
    22741 cgtgccgtgg gtacgccata tttttcttgc cagtaacacc atcacaatga cggcgaagat
    22801 ggcgtcggta gcgataccta tttccaccag ttccggtgct cgccaggcaa gaagcgccag
    22861 caccagatgg gagccgtttt ccatcaggca gtaaccaaaa atttgccgca ggatattgcg
    22921 ctggctgaca atgcacagca ggccaagcag aaaatgacct aacgctaccg ccagcgccgg
    22981 tttcagcccg gtagccatcg gtagcttcac gggctgaacg acaaatgcgc aaagcaggac
    23041 aattaacgcc gcgagcagtg ccatcattgc cggaccgaat aacgcttttt ccgggatgtt
    23101 ctggggaata tttcgtgcag cgtaagtcat gattaacggt accagcagca ctttggtgat
    23161 aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg aacaggcacg agagagtggc
    23221 gaaaatagac accagcacca gcgactggca ggcgtaaaat ccgcatgaca ggcgatagct
    23281 tttgacgcta atcacaaaca gcgatgtcag catcatcagt cccgccagat tatttacgat
    23341 catagaaccg gtcattcatc atctccttac tgcaaccagg tcgcggcgat tacgctggaa
    23401 atcagcgaca tcacaatcag tacaatcaac accaggcgca ttgaccctgg cagcggtgcg
    23461 gcatcggcga cggcctcgct cggtttgcca ggcacaacgc gaccaaacca gcgaataaac
    23521 caggcgaaac tggcgaccga ttcaatcatc agcagaatca tggcgggcag caggatccag
    23581 tactccactg acaacgcaaa accggcagca aacagcggga atttactgaa gaagccgttg
    23641 aacggcggca cgccggtaat cgccagcgct gccacgcaga aacccacgcc tggcaacggc
    23701 agggtgtgca atacgccacg cagacgcggc aacaagcgcg tgccgcagct gtaactcagc
    23761 gcacctgcta caaggaaaaa caggctttta gcgaacgcgt ggttgacgat gtaggcgatg
    23821 ctgccctcca gcgccagccg cgagccgaag atggagagcg acaaaccgaa gaacatccag
    23881 ccaagttgag tgatggtcga ccaggccagc aaccgcttca tatcctgctg tggcaaatac
    23941 atcagaaagc cataaagaat ggtgaccagc gccatgacca tgccaacgcc gccaatcaca
    24001 tgcgggatat tgccgccgtc gataatggcg cgggcaaaaa tgtaaacgcc cactttcacc
    24061 ttcgatgcgg cgtggagata ggcactgatc ggtgttggcg cttccattgc atccggtagc
    24121 cacgcttgca tcggtagctg ggccgatttc ccccacgcgg caaacaggat gccgccataa
    24181 accagataac gtgcgtcgcc gtgtaactcg ctcatcgcgc taagcgcaaa cgttccggtc
    24241 tgcaaaaaca gcgtggcggc ggcaagatac aaccccaacg agccgatatg agtgataagt
    24301 aacgctttta gtgctgaacg ctgtgcttta tcgctctggt aataactgat caacgcccag
    24361 gagcagccgc ccgtaatttc aaaaaacaac aactgaccga gcagcgtcga ggagagtacc
    24421 agtcccgcca tcgcgccgat aaacaacagc aaaaatgcgt aataacgatt cgtgccgtta
    24481 tgcgggtgtt cgcgattttt atccgtcaga taacccgtgg agtagatcgt gaccagcaaa
    24541 ccgagaaaca ccaccacaaa cagaatcagc gtactcacgc gatcaatgac caggccaaac
    24601 aatgccacat cccccacgtt gaccaatgtc aacgtgacgg caactttatc ggcctgataa
    24661 aacgcggaga tcagtgacaa catgcacagc gtggtcagcg cggcgaacaa aaccccccat
    24721 tcggcggccc gacgttgtgg cgaaaacgaa acgaccagtg cgccgataaa aggcagcaat
    24781 aacgtcgtca gagcaagatt ttccatattc cgtcagtgct ccttacagac cggttaacca
    24841 gaagacccat gcaagcgcag caaggctgaa gccaagccag gtcacatggt gaatgagtaa
    24901 aaaacgcccg cgtgccagcg tgttttctgc gattgaggcc agtacaaaaa tcagcaaaac
    24961 cttgagcagc gtaacgacaa gtgaagtcag caggcaggcg agagaaagtt cttgcgcgcg
    25021 cccaaaggac agaaacaggg ccacaaacag tgatgccatc acgacctgtt tcagccccag
    25081 cccccatttc gctagcgcca gcccggcacc ggaatattcg gtcagcgggc cttcctgtaa
    25141 ttcctgttct gcttcagcaa catcaaaggg aatttttccc atctcaatga agcaggcaaa
    25201 accacaggcc agtaacgcca gtacggtggt tagcggcgag ttccagccca tcgccagcgt
    25261 attgctgatc atctcgatat gcgtggaacc tgctatcagc gccaatacca gcagtgagag
    25321 aataagcatt ggttcgacca gaatgccgag cgtcaactcg cgactggcac cgactcccgc
    25381 aaacggactt ccggtatcca gcccggaaag agcaaagaaa aaacgaaaca gggcaagaag
    25441 atagataagg gtgatcagat cgccgccgcc cgcaaaaggg gaaacggtaa taaacagtgg
    25501 taaggccatc gccagcacca gcatgctgct gattaatacc cacggcatca ggcggaacat
    25561 cagacctgaa gatgtcggcg caacttcctg gcgtttaaac agtttgtgga tatcgcgata
    25621 atcctgccag atccccggcc cgcggcggga gtgcatacgc gcgcgtatct gccgggaaat
    25681 acccgtaaaa agtggggtta gcatcagcag aatcacggcc tgtgctaacg caaaaataac
    25741 cagatatccg tcgcaaagag tttgtctcat ggtgatttcc ttagacggca atagcgatta
    25801 gcagcacaac cagtgcggcg accacataca ggcaatagag acgaaagtcg ccgctttgca
    25861 gatgctgtat ttctttggcc agccgttggg tggcgctcac gatggggcgg atcacccgct
    25921 catcccagaa gggttctgtg ctctgagccc tggcggtgac gtgcgcaaaa cctttattta
    25981 gcctcagcgt agggtcgagc tgttgtcgta gacgaaataa cgcagagaag accacacgca
    26041 gcggctgcat cacgccattg cctgacgggg ccatcgcatt ttcccagccg tagccgcatg
    26101 cccaggcatc tcctgtgcga cgaaacgctg cgcggcgcga acgacaccac agccagtaaa
    26161 ggccaggcag caaaggcatc gccagtagta acagcaacag taatgagggg gtgacctgtg
    26221 tatgaaacgt gccgggtaca agtgctattc cgctggcgac agtgacggga ggggtattgg
    26281 taaacgcatg ggcaatatgc attatcttcg gtgccagcca actggcacta agcgcaatta
    26341 atacgcagag tgccgcgagc agtagcatgg cgacgatcat cgtatttggc acttcctgtg
    26401 cctcttcagc gtgtgtactg cgcggcgcac cacagaaagt aataccgtac atttttacga
    26461 agcacattac tgccagccca ccagtgactg ccagcattac catagcaata ggacccgcaa
    26521 gttgtagcgc tacggcttcc acacgactta gtgagaacag cgactgccag gtgtaccatt
    26581 cgctgataaa accattcagc ggaggaatgg ctgatatcgc gaggcaacca atcaggcatg
    26641 ctgcggctgt ccacggcatc cgtttcgcca gtgcccccat tttttccatg tcgtgggtat
    26701 gcaaacgcga aataatcgcg cccgcgccga gaaatagcag ccctttgaac agcgcatggt
    26761 tgagcagatg aaacagtgcg ccgagcagtc caaccacggt gagcagcggg tcgtgcagtg
    26821 acagaccgac catcgccaca ccgactgcca gcaaaataat gccgacgttt tcgacggtac
    26881 tccaggccag cagccgtttg atatcctgtt ccgccagcgc atacagcacg cccaggagcg
    26941 cggagattgc gccgatcgcc atcaccagaa tgccccacca cagcggcaaa cccgtttgcg
    27001 ccagcaggtc catcgctact ttcaggatgc cgaaaatacc gattttgacc attacgccag
    27061 acatcaacgc cgaagcgtgc gatggtgcgg cagggtgagc gcgcggcaac cagctgtgca
    27121 acggcatcat cccggctttc gcgccaaaac cgaaaaaggc cagcaggaac accgccgacg
    27181 ccagccccgg agaaagtgaa agcgtgcgga aactggcaaa atcgaggctg ccgctttcgc
    27241 gccccatcag caagaaggcg atcataatca gcaccgatcc ggcgtgggcg ataaaaaagt
    27301 agagcatgcc agcgttgatc gacgttttat cctgcctggc aatgaccaga aaccaggaag
    27361 acagcgacat catttcaaac agcacgatga accaaaaagc gttgtccatc accagcaggg
    27421 caaccatcga tgcgatgaaa agattcataa agaagcccat cgccgccgcg cctttgccct
    27481 cgtattcgcg catataagtc aatgaataga gcgaacaaac caccaccagc aaggagataa
    27541 ccagcaccat aaatgccgag aggctatcca ttcgcagggt aaaatcggca aagtcaaaag
    27601 gggtggcaaa acgggcgact aaagttacac cagcatgtaa ttgcgtaatt ccgctgatga
    27661 tgccaatcac gccgcccacc agcgatgtga tgccggaaag cttaatagcc agtctgtcca
    27721 gaccgagtaa aaacagcgaa gccagactgg caaacagata gaggatcagc gaccaggtta
    27781 ataattgcag ggcatccatt agcgttgctc ctgagtgagg gaaaggggat cctccccgcc
    27841 attggcaaac cagcttgcgg caaggcgctg tttttctttc atctgtcgtt gcaggctatc
    27901 gtcggtgatc agccgtaacg cctgattcgg gcaagcgcga acgcaggccg gaccttctgg
    27961 caagaaatca cacaggtcgc atttcaccgc gacggtctgg acacccggtt cccagcgcag
    28021 caatgcatgt tggggcggca cgttttcttc gccgtctttg agtgagcctt cagcctgaaa
    28081 aacatattgc gcaggggcat tcaccggacg gcttcctgaa gcgctgattg cgccaaatgg
    28141 gcagaccacg gcgcaaagct tgcagccaat acagaggctt tcgttgagtt ggatcgcatc
    28201 atccctctgg gagatggcat taaccgggca gacctgcagg caaggggctt cctcacagtg
    28261 atgacacacg acaggggcag tgagtgttga cgtcttcgcc agggccaggc gcgggtgttg
    28321 ctgtaaacct tgcgttttat gcacgtccga acaggcagcg agacaggtat tacatcctgt
    28381 acaccacagt ggttcggcca ccacaaagcg gttcatgtcg gctccttgaa aatgatatga
    28441 aacattgcat ttcatattgc ataaaggatg ccattctgat taactcaata tattcaataa
    28501 gttatgtatt gactggattc tgaacggtac aaaaatgatg gtcattaacg ttcagcaaaa
    28561 aatggattcg acacccgtgt cgcaggggaa agcgagaatg tgatcttgat cacgagaagt
    28621 gaggtatgct ggccgcaagc gcagccagca cggaatggag caaagtaatc aggcgtgttc
    28681 tttcagccag ttcagcacaa cgtcgtggtg attgctggtt ttgaaatcgt caaagacatg
    28741 ttcgattttg ccatcagcgt caatcaggaa gctgatgcga tgaatgccat cgtaggtttt
    28801 gcccatgaag gacttttcac cccagacgcc gaattgttcg cacacctggt ggtcctcatc
    28861 agacaggagc gtaaagttaa gcagctcttt ttccgcaaaa cgggagagtt tttcgggttt
    28921 atcggtgctg atacccagca catcaacgcc cgcttttttc aactcatcca tgttatcgcg
    28981 taagccgcag gcctgtacgg tacagccggg ggtcatggct ttcgggtaga aataaaccag
    29041 aacacgctgt ccctggaagt cggtcaaatt aacttgttct ccgtcttgat ccggcaagct
    29101 aaatttcggt gcgatatcac cggctttcag tggattcatt acttaactcc atcctgttca
    29161 tcatgttggg aataattgac gacgttaata ctgccttgtg cattgagttc tgtacatagg
    29221 gctttgaacg cttgctcaat atttgctgcg tccgcagatg cggggctgtg ggcggttatc
    29281 tgaatatgca actgcgcagc ccgttcattt tcagcaggtt gcgtgcgcga caccagctcc
    29341 gcaatgttca tatgatgcgc gtcgaaaagt gctgtgaagc gttcaattaa atgcggggag
    29401 tctgccacat cgacctgaac ccagacagat gctggcattg gcggacgcgg acgcgccgtc
    29461 gtgcgcttca tcacgattaa aagatccagt tcggcacctt tcaacggtaa cgttgattca
    29521 atcagagtga tggcattcca tgaaccggaa agcagcatga taaacgtgaa ctcttctccc
    29581 agcatcgcca ggcgactgtc ttcaatatta cagccgcaac tactgacatg acgggtgatg
    29641 gtgttcacaa ttccagggcg atcggcaccc aacgcagtga tcaccagata atgttgcgat
    29701 gacagtgtca aactggttat tcctttaagg ggtgagttgt tcttaaggaa agcataaaaa
    29761 aaacatgcat acaacaatca gaacggttct gtctgcttgc ttttaatgcc ataccaaacg
    29821 taccattgag acacttgttt gcacagagga tggcccatgt tcacgggaag tattgtcgcg
    29881 attgttactc cgatggatga aaaaggtaat gtctgtcggg ctagcttgaa aaaactgatt
    29941 gattatcatg tcgccagcgg tacttcggcg atcgtttctg ttggcaccac tggcgagtcc
    30001 gctaccttaa atcatgacga acatgctgat gtggtgatga tgacgctgga tctggctgat
    30061 gggcgcattc cggtaattgc cgggaccggc gctaacgcta ctgcggaagc cattagcctg
    30121 acgcagcgct tcaatgacag tggtatcgtc ggctgcctga cggtaacccc ttactacaat
    30181 cgtccgtcgc aagaaggttt gtatcagcat ttcaaagcca tcgctgagca tactgacctg
    30241 ccgcaaattc tgtataatgt gccgtcccgt actggctgcg atctgctccc ggaaacggtg
    30301 ggccgtctgg cgaaagtaaa aaatattatc ggaatcaaag aggcaacagg gaacttaacg
    30361 cgtgtaaacc agatcaaaga gctggtttca gatgattttg ttctgctgag cggcgatgat
    30421 gcgagcgcgc tggacttcat gcaattgggc ggtcatgggg ttatttccgt tacggctaac
    30481 gtcgcagcgc gtgatatggc ccagatgtgc aaactggcag cagaagggca ttttgccgag
    30541 gcacgcgtta ttaatcagcg tctgatgcca ttacacaaca aactatttgt cgaacccaat
    30601 ccaatcccgg tgaaatgggc atgtaaggaa ctgggtcttg tggcgaccga tacgctgcgc
    30661 ctgccaatga caccaatcac cgacagtggt cgtgagacgg tcagagcggc gcttaagcat
    30721 gccggtttgc tgtaaagttt agggagattt gatggcttac tctgttcaaa agtcgcgcct
    30781 ggcaaaggtt gcgggtgttt cgcttgtttt attactcgct gcctgtagtt ctgactcacg
    30841 ctataagcgt caggtcagtg gtgatgaagc ctacctggaa gcggcaccgc ttgcggagct
    30901 tcatgccccg gctggaatga ttttgccggt gacctccggt gattatgcaa tcccggtgac
    30961 caacggtagt ggtgctgtcg gtaaggcgct ggacattcgt ccaccagccc agccgctggc
    31021 actggtttct ggcgcgcgta cccagttcac gggcgatacc gcttcattgc tggtggaaaa
    31081 tggtcgtggc aatactctgt ggccgcaggt ggttagcgtg ctgcaggcga aaaactacac
    31141 catcacccaa cgtgatgatg ctggtcagac actgaccacc gattgggtac aatggaaccg
    31201 tctggacgaa gacgagcagt atcgtggtcg ttatcaaatc tctgttaagc cgcagggtta
    31261 tcagcaggcg gttacggtta aactgctgaa cctggaacag gcgggcaaac cggttgcaga
    31321 cgcggcttcc atgcagcgtt acagcacgga gatgatgaac gttatttccg ccggtctgga
    31381 taaatctgcc actgacgccg cgaacgctgc gcaaaatcgt gcctccacca ctatggacgt
    31441 acaaagtgca gctgatgaca ccggtttacc aatgctggtc gtacgcgggc cgttcaatgt
    31501 ggtttggcaa cgtctgccag cggcgctgga aaaagtgggc atgaaagtga ccgacagcac
    31561 ccgttcgcag ggcaacatgg ccgtaactta taagccgctg tctgacagcg actggcagga
    31621 actgggcgcg agcgatccag gcctggcatc cggtgactat aaactgcagg ttggcgattt
    31681 agataaccgc agcagcctac agttcatcga tccgaaaggt catactctga ctcagagtca
    31741 gaacgacgcg ctggtagctg tcttccaggc tgcgtttagc aagtaaaaat acagggctgg
    31801 aatcatccgg cccttttttc tgatatgata cgcaaacgtg tgcgtcggca ggaaaacgcg
    31861 attttagcgg taattcgcac gaaatttgtt tgtcggacgt agttcggata aggcgttcac
    31921 gccgcatccg acaaaacatc cggcacacca gacagcaaaa gattttaaaa cgttaattca
    31981 cacccaggag tgataaagat gcaaaagcaa gctgagttgt atcgtggtaa agcgaaaacc
    32041 gtatacagca cggaaaaccc ggacctgttg gtgctcgaat tccgcaatga tacgtcagca
    32101 ggggatggcg cgcgcattga gcagtttgat cgcaaaggta tggtgaacaa caagttcaat
    32161 tacttcatta tgagcaaact ggctgaagcg ggtatcccga ctcaaatgga gcgtctgctc
    32221 tccgataccg aatgtctggt gaaaaagctg gatatggtgc cggttgagtg tgtcgtgcgt
    32281 aaccgtgctg ctggctctct ggtgaaacgt cttggaatcg aagaaggtat tgagctgaac
    32341 ccgccgctgt tcgatctgtt cctgaaaaac gacgccatgc acgatccgat ggtcaacgaa
    32401 tcttactgcg aaacctttgg ctgggtgagc aaagagaacc tggcgcgtat gaaagagctg
    32461 acctacaaag cgaacgacgt gctgaaaaaa ctgttcgatg atgctggtct gattctggtc
    32521 gacttcaagc tggagtttgg cctgtacaaa ggtgaagtgg tactgggcga tgagttctcc
    32581 ccggacggca gccgcctgtg ggacaaagaa acactggaga aaatggacaa agaccgtttc
    32641 cgccagagcc tcggtggcct gatcgaagcc tatgaagccg tcgcccgccg cctgggtgta
    32701 cagctggact gatttttctg gttgcttgtt tctttagccg gagggtactc cggctaaatg
    32761 tctttgatac ccccgctaca atacttctca ttatctccct ttgacaggaa agtcatacca
    32821 ggccaatatg tatatacata acgtatatcc caaaaagggg gggctatgga aactactgta
    32881 tttttaagta atcgcagtca ggccgtcaga ttaccgaaag cggtcgctct gccagaagac
    32941 gttaagaaag tagaaattat tgcgattggt cgtacaagaa tcatcactcc cgccggtgag
    33001 tcctgggaca gttggtttga tggcgaaaat gtgagtgcgg attttatgga tatccgtgat
    33061 caacccgcaa tgcaggaaag ggaatcattt tgatgctgaa attcatgctg gataccaata
    33121 tctgtatttt cactattaag aataaaccag tacatgtcag agagcggttt aacctgaatt
    33181 ctggccggat gtgtataagt tctgtaacat tgatggagct tatctatggg gcagaaaaaa
    33241 gtcagatgcc agaacgcaat ttggcggtta ttgaaggttt cgtatctcgt ttggtagtgc
    33301 ttgattatga tagtgctgct gcgacgcata ccgggcaaat cagggccgag cttgcccggc
    33361 agggaaggcc tgttggacct tttgaccaga tgatagcggg acatgctcgc agtcgcggtt
    33421 tgattgtggt taccaacaat acccgagagt ttgagcgagt ggcgggaatc agactagaag
    33481 actggagcta acattctctt ttacttctaa cattcgtaaa ttatataacg tggcatgaag
    33541 ccttaattcc tccgccattt catcccgctt tcctgtggta atcttcctct gaaccaccta
    33601 cgatcatttc tataatgaat atattgttga ggtatctatg cgttggcaag ggcgacgtga
    33661 aagtgacaat gttgaagaca ggcgcaacag ctccggtggt ccgtctatgg gcggtcccgg
    33721 ttttcgcctg ccaagcggta aaggcgggtt gattttactg ttagtcgtgc tggttgcagg
    33781 ctactatggt gttgatttaa ccgggttaat gactggacag ccggtttccc aacaacaatc
    33841 aacgcggtcc attagcccta atgaagacga agctgcaaaa ttcacctcgg tgattctggc
    33901 aaccacggaa gacacctggg gacaacagtt cgagaagatg ggtaagacct atcagcaacc
    33961 gaagctggtc atgtaccgtg gaatgacgcg caccggctgc ggggctggtc agtccattat
    34021 ggggccgttc tattgcccgg cggatggcac agtttatatc gatctctcct tctatgatga
    34081 catgaaagac aaacttggcg cggatggcga ttttgcccag gggtacgtta tcgcccatga
    34141 agtcggtcat catgtgcaga aactgttagg catcgagccg aaagttcgtc aactgcaaca
    34201 aaacgcgacg caggcggaag tgaaccgctt atctgtgcgt atggaactcc aggccgactg
    34261 ttttgccggt gtctgggggc atagtatgca gcagcaaggc gttctggaaa ccggcgatct
    34321 ggaagaggcg ctgaacgcgg cgcaggccat cggcgatgac cgtttacaac agcaaagtca
    34381 ggggcgagta gtaccagata gtttcacaca tggcacttct cagcaacgct acagctggtt
    34441 taaacgtggt ttcgacagcg gcgatccggc acaatgcaat acttttggta aaagcattta
    34501 acttccgggg cagggatggc tgaactgact gcgcttcaca cattaacagc gcaaatgaaa
    34561 cgtgaaggga tccgccgctt gctggtgttg agcggggaag agggttggtg ttttgatcat
    34621 gcgcttaagt tgcgtgatgc cttacctggc gactggctgt ggatttcgcc gcagccagat
    34681 gctgaaaacc actgttcacc ctcggcgcta caaactttac ttgggcgcga gttccggcat
    34741 gcggtattcg acgcccgcca cggctttgat gccgctgcct ttgcagcact tagcggaacg
    34801 ttgaaagcgg gaagctggct ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa
    34861 cctgatgccg actcgctgcg ctggagtgat tgccctgacc ctattgcgac gccgcatttt
    34921 gtccagcatc tcaaacgcgt acttacggcg gataacgacg ctatcctctg gcggcaaaac
    34981 cagccgttct cgttggcgca ttttactccc cgtactgact ggcaccccgc gaccggcgca
    35041 ccacaaccag aacaacagca actcttacag cagctactga ccatgcctcc gggcgtggca
    35101 gcggtaacgg ctgcgcgtgg gcgcggtaag tcggcgttgg cagggcaact catttctcgt
    35161 attgcgggca gtgcgattgt caccgcgccc gcaaaagcgg caacggatgt actggcacaa
    35221 tttgcgggcg agaagtttcg ctttattgca ccggatgcct tgttagccag cgatgagcaa
    35281 gccgactggc tggtggtcga cgaagccgca gccatacctg cgccgttgtt gcatcaactg
    35341 gtatcgcgtt ttcctcgaac gttgttaacc actacggtgc agggctacga aggcaccgga
    35401 cgtggttttt tgctgaaatt ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa
    35461 cagccgatcc gctgggcaca gggatgcccg ctggaaaaaa tggttagtga ggcactggtt
    35521 tttgacgatg aaaacttcac ccatacacca caaggcaata tcgtcatttc cgcatttgaa
    35581 caaacgttat ggcgaagcga gccagaaacg ccgttaaagg tttatcagtt attgtctggt
    35641 gcgcactacc ggacttcgcc gctggattta cgccggatga tggatgcacc agggcaacat
    35701 tttttacagg cggctggcga aaacgagatt gccggagcgc tgtggctggt ggatgagggg
    35761 ggattatctc aacaactcag tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat
    35821 ctggtggccc agtcgctggc ggcgcacggt agcaatccac tggcagcgac attgcgtgga
    35881 cggcgggtca gccggatagc agttcatccg gcgcgtcagc gggaaggcac agggcagcaa
    35941 cttattgctg gtgctttgca atatacgcgt gacctcgact atctttcggt gagttttggt
    36001 tacaccgggg agttatggcg tttctggcac cgctgcggtt ttgtgctggt gcggatgggt
    36061 aatcatcgtg aagccagcag cggttgctat acggcgatgg cgctgttacc gatgagtaat
    36121 gcgggtaaac aactggctga acgtgagcat taccgtttac gtcgcgatgc gcaagctctc
    36181 gcaaagtgga atggcgaaac gcttcctgtt gatccactaa acgatgccgt cctttctgac
    36241 gacgactggc ttgaactggc cggttttgct ttcgctcatc gtccgctatt aacatcgtta
    36301 ggttgcttat tgcgtctgct acaaaccagt gaactggcat taccggcgct gcgtgggcgt
    36361 ttacagaaaa acgtcagcga cgcgcagtta tgtaccacac ttaaactttc aggccgcaag
    36421 atgttactgg tccgtcagcg ggaagaggcc gcgcaggcgc tgttcgcact taatgatgtt
    36481 cgcactgagc gtctgcgcga tcgcataacg caatggcaat tttttcactg actccttcaa
    36541 ttttctccca tggtcattgt gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt
    36601 cgccatgaaa catgaccatt ttgttgttca aagcccggat aaacctgcgc aacagttgtt
    36661 actgcttttc cacggtgtcg gggataaccc ggtggcaatg ggggaaatag gtagctggtt
    36721 tgcaccgctg tttcctgatg cgctggtggt gagtgtcggt ggcgcggagc cgagcggtaa
    36781 tccggcgggg cgtcagtggt tttcagtgca gggtattacg gaagataatc gccaggcgcg
    36841 tgtggatgcc atcatgccga cgtttattga aacggtacgc tactggcaga aacagagcgg
    36901 agtaggggct aacgccacgg cgctaattgg tttctcacaa ggcgcgatta tggcgctgga
    36961 gagcattaaa gccgaaccgg gccttgcttc gcgcgtcatt gcctttaacg gacgctatgc
    37021 gagcctgccg gagaccgcgt ctacagccac gacaattcac ttgattcatg gtggtgaaga
    37081 tccggttatc gatctggcgc atgcagttgc ggcgcaggaa gcgttaatca gtgccggtgg
    37141 tgacgtgacg ctggatatcg tggaggattt aggccatgcg atcgacaatc gcagtatgca
    37201 atttgccctg gatcacttgc gttataccat tccgaaacat tatttcgacg aagcgctaag
    37261 tggcggtaag cctggcgatg atgatgtgat tgaaatgatg taaaagacag attacttggc
    37321 ctgataagac gcgtgagcgt cgcatcaggc atcgttcacc attgccggat gcggcgttaa
    37381 cgccttatcc ggcctacggt tgagcactca gcttacttct tcggttgatc ctttttcggc
    37441 cagtcgtcgt catcgtccca cttatcgttg aaatcacgat gcgggggaag ctccggcttg
    37501 ttggcgagaa atttcttatg gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg
    37561 cctaccaaaa agacaatcac cagaatccac caatatttag ccagccagtc catgcttatt
    37621 tcctcttgca gaaccactca tcaggcgacg agctgttcca tgatacgttg atacatacgg
    37681 gcaagtagct gcaggttggc agcgttcaca cattcattaa ttttatgaat agtggcattg
    37741 accggcccga gttccaccac ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt
    37801 ccgcctgtgg tcagtagctg cggtttaatt tcattatagt gctcaaccgc gttaacgacc
    37861 gcatccacca gtttaccgcg cgcggtcaaa aatggctgcc cggaaagcca ccagtccacc
    37921 gtataacgca gttgatgttt ttcaagcagg gcaagcacct gtgctttgat catctcatca
    37981 gtcagttcgg tgctgaagcg gaagttaaac tgcacaaaca gttcacccgg aataacgttg
    38041 ttactgcccg ttcccgcctg aatattggca atctgcatac tggtcgccgg gaagaattca
    38101 ttgccctgat cccactcaat agccactaat tcattaagga aaggtgctgc gcgatgtacc
    38161 ggattgtcag ccagatgcgg gtaggcaaca tgcccctgaa cgccatgaat ggtgaggttg
    38221 caggttaacg atccgcgacg accatttttc accacatcac ctaccacttc gatactcgac
    38281 ggttcgccga ccaggcagta atcgagacgc tcattacgtg ccattaacgc ttcgacgact
    38341 tttaccgtac cgttgtgtgc actggcttct tcatcagagg tgatcagaaa tgccagtcgc
    38401 cccgtatggt tgggatgttg tgcgacaaag cgttctgccg ctaccaccat cgccgccagc
    38461 gagcctttca tatctgccgc accgcgcccg aataacatgc catcacgaat ggtgggttca
    38521 aacggcggat tgatccaacg atcggcgtcg ccaggcggca ccacgtcggt atgcccggca
    38581 aaggctaacg tttcaccttg cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc
    38641 atgcgttcaa cggtaaaacc gatcgcctgc aaacgttcaa tcaacaaagc ctggcatcct
    38701 gcgtcatcag gactcaggga agggcggcga ataagctgtt gtgtcagctc aataaccggg
    38761 cacgacatag actacacctc atggaaaaat tgctgataac tggaatcact gaaacccagc
    38821 agcataggct taccaggcgc gcagagcaat ggacgtttga taattgcagg catttcagtc
    38881 attaatgccg ccgcagaggc cgcatcggtg attttattgc gggtggtttc gtccagttta
    38941 cgccaggttg taccacgggt gttgagtaac gcttcccagc ctaattcgtt gataaaaccg
    39001 ttcaataatt cgctgtccag cccatcgacg cggtaatcat gaaaacgata gtcgatgtta
    39061 ttggcttcca gccaacggcg agccttttta atggtgtcac aatttttgat gccgtaaagt
    39121 gtaaccataa tgaatccttt ttcgatttat gagaataaat actcatttaa gggtgaaaac
//
LOCUS       DAZKGS010000025        38684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKGS010000025 DAZKGS010000000
VERSION     DAZKGS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..502
                     /gene="yfdR"
                     /locus_tag="ACHOQY_001328"
     CDS             <1..502
                     /gene="yfdR"
                     /locus_tag="ACHOQY_001328"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9677466.1"
                     /translation="YYDKINKDDIVINDIAVSLSNICRFAGHLSHFYSVAQHAVLCSQ
                     LVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIREKYGLPPVMSTP
                     VKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHAYGMFMERFNELS
                     GLRKCA"
     gene            493..639
                     /locus_tag="ACHOQY_001329"
                     /pseudo
     CDS             493..639
                     /locus_tag="ACHOQY_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            616..1080
                     /locus_tag="ACHOQY_001330"
                     /pseudo
     CDS             616..1080
                     /locus_tag="ACHOQY_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1082..1273
                     /locus_tag="ACHOQY_001331"
     CDS             1082..1273
                     /locus_tag="ACHOQY_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677467.1"
                     /translation="MNGQISIVRPGACDDSEIRMIIRLARGKTITVLITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            1276..1866
                     /locus_tag="ACHOQY_001332"
     CDS             1276..1866
                     /locus_tag="ACHOQY_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9677468.1"
                     /translation="MTTFTNSKLTDEYVSNATLIRLILWADQHNSHYVVAALRELQER
                     RKADSAEPVSQTYKLPVNTPCQDAPAHIWLQTAGVWPEDGELSELTWCSHNQHHDDTL
                     YVRADLVNGNYPATPDGWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGD
                     WFNDGKPTWDDGDGEDLYLKEVTHWMQLPEPPQEVK"
     gene            1954..2196
                     /locus_tag="ACHOQY_001333"
     CDS             1954..2196
                     /locus_tag="ACHOQY_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9677469.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2200..2334
                     /locus_tag="ACHOQY_001334"
     CDS             2200..2334
                     /locus_tag="ACHOQY_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677470.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVAKRMFDVWCHCRG
                     "
     gene            2353..2607
                     /gene="xisR"
                     /locus_tag="ACHOQY_001335"
     CDS             2353..2607
                     /gene="xisR"
                     /locus_tag="ACHOQY_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9677471.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2641..3928
                     /locus_tag="ACHOQY_001336"
                     /pseudo
     CDS             2641..3928
                     /locus_tag="ACHOQY_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3949..4650
                     /gene="mlrA"
                     /locus_tag="ACHOQY_001337"
     CDS             3949..4650
                     /gene="mlrA"
                     /locus_tag="ACHOQY_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9677472.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            4710..4817
                     /gene="yohO"
                     /locus_tag="ACHOQY_001338"
     CDS             4710..4817
                     /gene="yohO"
                     /locus_tag="ACHOQY_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9677473.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4798..5529)
                     /gene="yehW"
                     /locus_tag="ACHOQY_001339"
     CDS             complement(4798..5529)
                     /gene="yehW"
                     /locus_tag="ACHOQY_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9677474.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5534..6460)
                     /gene="yehX"
                     /locus_tag="ACHOQY_001340"
     CDS             complement(5534..6460)
                     /gene="yehX"
                     /locus_tag="ACHOQY_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9677475.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6453..7610)
                     /gene="yehY"
                     /locus_tag="ACHOQY_001341"
     CDS             complement(6453..7610)
                     /gene="yehY"
                     /locus_tag="ACHOQY_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9677476.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7617..8534)
                     /gene="osmF"
                     /locus_tag="ACHOQY_001342"
     CDS             complement(7617..8534)
                     /gene="osmF"
                     /locus_tag="ACHOQY_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9677477.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8745..11042)
                     /gene="bglX"
                     /locus_tag="ACHOQY_001343"
     CDS             complement(8745..11042)
                     /gene="bglX"
                     /locus_tag="ACHOQY_001343"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9677478.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11238..12953
                     /gene="dld"
                     /locus_tag="ACHOQY_001344"
     CDS             11238..12953
                     /gene="dld"
                     /locus_tag="ACHOQY_001344"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9677479.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12991..13923)
                     /gene="pbpG"
                     /locus_tag="ACHOQY_001345"
     CDS             complement(12991..13923)
                     /gene="pbpG"
                     /locus_tag="ACHOQY_001345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9677480.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14097..14684)
                     /gene="yohC"
                     /locus_tag="ACHOQY_001346"
     CDS             complement(14097..14684)
                     /gene="yohC"
                     /locus_tag="ACHOQY_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9677481.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14854..15432
                     /gene="yohD"
                     /locus_tag="ACHOQY_001347"
     CDS             14854..15432
                     /gene="yohD"
                     /locus_tag="ACHOQY_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9677482.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15562..16323)
                     /gene="yohF"
                     /locus_tag="ACHOQY_001348"
     CDS             complement(15562..16323)
                     /gene="yohF"
                     /locus_tag="ACHOQY_001348"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9677483.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16376..17812)
                     /gene="mdtQ"
                     /locus_tag="ACHOQY_001349"
     CDS             complement(16376..17812)
                     /gene="mdtQ"
                     /locus_tag="ACHOQY_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9677484.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            18036..18119
                     /gene="yohP"
                     /locus_tag="ACHOQY_001350"
     CDS             18036..18119
                     /gene="yohP"
                     /locus_tag="ACHOQY_001350"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9677485.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18507..19457)
                     /gene="dusC"
                     /locus_tag="ACHOQY_001351"
     CDS             complement(18507..19457)
                     /gene="dusC"
                     /locus_tag="ACHOQY_001351"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9677486.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19696..20094
                     /gene="yohJ"
                     /locus_tag="ACHOQY_001352"
     CDS             19696..20094
                     /gene="yohJ"
                     /locus_tag="ACHOQY_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9677487.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            20091..20786
                     /gene="yohK"
                     /locus_tag="ACHOQY_001353"
     CDS             20091..20786
                     /gene="yohK"
                     /locus_tag="ACHOQY_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9677488.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20916..21800
                     /gene="cdd"
                     /locus_tag="ACHOQY_001354"
     CDS             20916..21800
                     /gene="cdd"
                     /locus_tag="ACHOQY_001354"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9677489.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21950..22669
                     /gene="sanA"
                     /locus_tag="ACHOQY_001355"
     CDS             21950..22669
                     /gene="sanA"
                     /locus_tag="ACHOQY_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9677490.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22672..22911
                     /gene="yeiS"
                     /locus_tag="ACHOQY_001356"
     CDS             22672..22911
                     /gene="yeiS"
                     /locus_tag="ACHOQY_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9677491.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            23230..24468
                     /gene="preT"
                     /locus_tag="ACHOQY_001357"
     CDS             23230..24468
                     /gene="preT"
                     /locus_tag="ACHOQY_001357"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9677492.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            24462..25697
                     /gene="preA"
                     /locus_tag="ACHOQY_001358"
     CDS             24462..25697
                     /gene="preA"
                     /locus_tag="ACHOQY_001358"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9677493.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25768..26778)
                     /gene="mglC"
                     /locus_tag="ACHOQY_001359"
     CDS             complement(25768..26778)
                     /gene="mglC"
                     /locus_tag="ACHOQY_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9677494.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVVLGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26794..28314)
                     /gene="mglA"
                     /locus_tag="ACHOQY_001360"
     CDS             complement(26794..28314)
                     /gene="mglA"
                     /locus_tag="ACHOQY_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9677495.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(28375..29373)
                     /gene="mglB"
                     /locus_tag="ACHOQY_001361"
     CDS             complement(28375..29373)
                     /gene="mglB"
                     /locus_tag="ACHOQY_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9677496.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(29653..30693)
                     /gene="galS"
                     /locus_tag="ACHOQY_001362"
     CDS             complement(29653..30693)
                     /gene="galS"
                     /locus_tag="ACHOQY_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9677497.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(30835..31992)
                     /gene="yeiB"
                     /locus_tag="ACHOQY_001363"
     CDS             complement(30835..31992)
                     /gene="yeiB"
                     /locus_tag="ACHOQY_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9677498.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLCYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(32009..32677)
                     /gene="folE"
                     /locus_tag="ACHOQY_001364"
     CDS             complement(32009..32677)
                     /gene="folE"
                     /locus_tag="ACHOQY_001364"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9677499.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32935..33771
                     /gene="yeiG"
                     /locus_tag="ACHOQY_001365"
     CDS             32935..33771
                     /gene="yeiG"
                     /locus_tag="ACHOQY_001365"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9677500.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33803..35794)
                     /gene="cirA"
                     /locus_tag="ACHOQY_001366"
     CDS             complement(33803..35794)
                     /gene="cirA"
                     /locus_tag="ACHOQY_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9677501.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(36087..37556)
                     /gene="lysP"
                     /locus_tag="ACHOQY_001367"
     CDS             complement(36087..37556)
                     /gene="lysP"
                     /locus_tag="ACHOQY_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9677502.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37761..38642)
                     /gene="yieE"
                     /locus_tag="ACHOQY_001368"
     CDS             complement(37761..38642)
                     /gene="yieE"
                     /locus_tag="ACHOQY_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9677503.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
BASE COUNT         9619 a        10156 c         9661 g         9248 t
ORIGIN      
        1 ttattatgac aagataaata aagacgacat cgttattaac gatatcgcgg tttccctttc
       61 aaatatctgt cgctttgcag gacatctttc acacttctac agcgtcgccc aacatgcggt
      121 gctttgcagc cagctggtac cgcaggaatt tgcttttgaa gcgttaatgc atgatgcaac
      181 agaagcatat tgccaggaca tccccgcacc actgaaacgc cttcttcctg actataaacg
      241 gatggaagaa aaaatagacg ccgtaatccg tgagaaatac gggttacctc ctgttatgag
      301 cacgccagtg aaatatgccg atctcattat gctggcaacc gaacgccgcg atctcgggct
      361 tgatgatggt tctttctggc ctgtactgga aggtatcccg gcgacagaga tgttcaaagt
      421 tattccactg tcaccaggcc atgcctacgg gatgtttatg gaacgtttta acgagttatc
      481 ggggttacgc aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg cgggaaatgt
      541 gtaccgcgag atctgaaagt gaacgaaaca aacgctgagt acctagtgcg taaatttgct
      601 gaagctgagg ccaagtgcgc ggcgctggct gaacgtatcg aagagttaca gacaaaacct
      661 acgccagatt cgtttggcat catcggtgaa aatattcgaa cacaggataa tcgaataacg
      721 tcagacccta tgttttgtgt gtatcaaaag cgcgaaattg ttgttgatgc tgattatgac
      781 tatgaccgga ttgtctgggt tgatgaagat ggcaatgaag ccaataaact ccaaagtcgt
      841 cgtctcgaac tacttcatga aaactttcgg gaaccaccag aaaaatggcg gcgcgttgct
      901 gtgaaagata ttgatgaatt cgttacctgc tgtttcaccg aacagggttg taaagactac
      961 ctggcagcca atggtcacaa tcttcgcttg ccatttatat atgtaaaaag cggtttcagg
     1021 aacgctgaat atatcggcat aagaacctgg cttgctggca ttcgcatcaa aggagagtga
     1081 gatgaacgga caaatatcaa ttgttcgacc aggagcatgt gacgatagcg agatacgcat
     1141 gattattcgt ctggcgaggg ggaaaacaat aactgttctc attactccag aaaatctcgc
     1201 attagcatta acaggaaagt cagacctgcc agtagagcta aagctgcgaa atgttgagat
     1261 taaggtgaaa tagctatgac cacttttacc aatagcaaac taacagacga atacgtttca
     1321 aatgcaacgt tgattcggct cattctgtgg gctgaccagc acaatagcca ttatgttgta
     1381 gcggctctgc gcgagctaca ggagcgccgc aaggctgata gtgcagaacc tgtaagccaa
     1441 acttacaagt tgccagttaa tacaccttgc caagatgcgc cagcccatat ctggctgcaa
     1501 acggctggag tatggccaga agatggcgag ttaagcgaat taacgtggtg cagccacaat
     1561 caacaccatg atgacacgct atatgttcga gctgaccttg tgaatggcaa ctatccggca
     1621 actccggatg gttggataag ctgtagtgag cgaatgcctg aaatgggaga gcgacaatgc
     1681 tatgtgttag cagctgactt taaaaacaac tacccaccaa acatccccaa cactcaggtc
     1741 ggcgtatatg gcgactggtt taatgatggc aagcccactt gggatgacgg tgatggcgaa
     1801 gacctgtatc tcaaagaggt aacccactgg atgcagctac cagaaccgcc gcaggaggtg
     1861 aaatgatgaa ttggcctgaa gcattcaccg cagttggagt tgcaatagcg gtggcattta
     1921 ttctgtattc gcttttccgc tggggataaa ggaatgttcg ctctgattca acgtggtcag
     1981 atatacacgg acagagccgg ataccctgtg gtgattactc gcagtactca gcactcagtg
     2041 ttctttcgac gcatggacgg gcgctccgga cgggtacgca ttggtgagtt caacaacctg
     2101 ttcgaacata ttgaccaaca ggagtaccgc aaaattctgg cgggcactga gcaggaaatg
     2161 cgcctgaaaa aattacgcgc aatgcaacgg aggtgataca tgcatacggc ttttgagttc
     2221 tgggttcgca agacattcgg caatcgctac gacctgaccc gtgatgtcga cggcttctac
     2281 tgccgtgaag ttgcgaaacg aatgtttgac gtgtggtgcc actgccgtgg atgaaagttt
     2341 tatgaggttg gcatgcagac aatcatctat cagataaccc ccagcaaatg gtgtacggag
     2401 agagtcctta ttgcatcaac agggctaaag cccggcacca tcgagcgggc cagaagaaag
     2461 tcatggatgc agggaaaaga ataccgccat tacgctgtag aaggtgatcc tgggcattac
     2521 agtgaatgcc tgtacaacat cgaagaaatt atgcgatgga tcgaaaacca gaaacaacca
     2581 ggtgccaaaa atgcaagttc cggttaacct gttaatgctc ctggacgtct gggaggttta
     2641 atgagtaacg catcataccc gacaggcgtt gaaaaccatg gaggatcact ccgtatatgg
     2701 tttcactata atggcaaacg tgtcagagaa aacctcggtg ttcctgacac agacaaaaac
     2761 cggaagatcg ctggtgaact tcgcacttcc gtttgttttg caatcagaat ggggagtttc
     2821 gactacgccg cgcagttccc taattcccct aacctgaaac actttggtct gggaaaaaga
     2881 gagataaccg ttaaggcact ttcggaaaaa atggttggac cttaagaaaa ttgagatttg
     2941 tgcgaatgca cttaaccgtt accagtcagt aattaaaaac atgttaccaa tgttaggtga
     3001 aaaaaaactg gtttcatcca taacaaaaga ggatttactt ttcgtaagga gagatttgtt
     3061 gaccggttac caaaagcttt ctaatggaaa gacttcttcc ataaaagggc gctcagtggt
     3121 cacggtaaac tactatatga caaccatagc tggaatgttt caatttgcaa cagataatgg
     3181 ttatacctca ggaaacccat ttaacggtct ggctccctta aaaaagtcca aggtaaaacc
     3241 agatcctctc acccgtgacg aatttattcg ttttattgag gcttgccgtc atcaacaaac
     3301 aaaaaacctg tggattctcg ctgtatacac gggtattcgt cacggggagt tggtatcgct
     3361 ggcatgggaa gatatagacc ttaaagcaag gactataacc atccgtagaa attatacaaa
     3421 acttggcgaa ttcactccac caaaaaccga tgcaggcacc ggaaggacaa ttcatctggt
     3481 tcaaccagct attgatgctc ttaaaagcca ggcggaaatg accatgcttg gaaagcaaca
     3541 ttctgtagag gtgaagcaga gggaatatgg gagaactgct gtgcataaat gcacttttgt
     3601 ttttagtcct caggtaacaa aacagcagca gttgtccgga cctcactaca aggttgactc
     3661 catcagggag tcatggacaa gtatcttaaa acgcgcaggt ctgagacaca gaaaatcgta
     3721 ccaatccagg catacttatg catgctggtc acttgccgca ggagctaatc ctagttttat
     3781 cgcaagccag atgggccaca caaacgcaca aatggtattc aatgtttacg gagcatggat
     3841 gaaagacaac aatcacgaac agatagaact ccttaacaaa agactatctg aaagtgtccc
     3901 atgtatgccc cataagaaag cagggtaaaa taaaaacttg caaaatcaat tagtttaccc
     3961 ttaatccctg tcacgttacg cgcgtggcag aggcgttacg ggttgctgaa accgcaacgg
     4021 acagacggcg gtcatcgact gttcaacgat gccgatattg accgtatccg cgagatcaaa
     4081 cgctggatcg acaacggcgt gcaggtcagc aaagttaaaa tgctgctcag taatgaaaat
     4141 gttgatgtgc agaacggctg gcgcgatcag caagaaacat tactgaccta cctgcaaagc
     4201 ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
     4261 acactcacca cacatctgtt tattcctctg cgccgacgac ttcaatgcca acaaccgact
     4321 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
     4381 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
     4441 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
     4501 ctcgcccact cgctcaatca actacgccct gaactattcg aaggtcgtac attgctggtg
     4561 tggtgcggtg aaaatcgaac ctccgcccaa cagcagcaac tcaccagttg gcaagaacaa
     4621 ggccatgata ttttcccact cggcatttaa tgattcgtta acaaatgcgc tttactgtac
     4681 aatcctttcg ttaacataag gagtgcatta tgcgcatagc taaaattggg gtcatcgccc
     4741 tgttcctgtt tatggcgttc ggcggaattg gtggcgtcat gctcgcaggt tataccttta
     4801 ttttgcgtgc tggctaagcg cctgcaccag cctttcaaac aggcggtctg cgatgatcgc
     4861 cgccagtgcc accagtaacg ccccctggat cacatacgcg gtattaaatc cgctaagccc
     4921 gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt gaggcgatcg tcgccgtacc
     4981 aatgttgata atcaccgaag ttcgcacgcc cgccagaatc accggagccg ccagcggtag
     5041 ctcgacctta cgcagtcgct gaccacgact cattcccata cctttcgcaa cttctgtcac
     5101 gctggcatcg atcgctccca gcccggcaag tgtcgcctgc aggacgggca gcacaccgta
     5161 aaggatcaag gcgataatcg ctggttgcag accaaagccg atcaccggaa cggcaatcgc
     5221 cagcactgcg acaggcggaa aagtctgtcc aacggcggca atagtttcca ccagtgggcg
     5281 aaattccgcg ccccacgggc gagtgacagc aattccggca ccagtgccaa tgatcaccgc
     5341 aaacaaactc gaaattccca ccagccagaa atgagccagt gccagagctg caaaactttc
     5401 ttgctgataa acgggtcgtg gcagttgtgg gaacaaggca gcaaacagcg gctggctgta
     5461 aggcagccag aaaatcagtg ccacaaacaa agcaatgagc cagaacagcg gatcgcgcaa
     5521 catcttcata cgcttacgcc tccaccagca gatcctgaaa atgcagcgta ccgcaaggct
     5581 ggccctgcgt gttcaccacc ggcagcacct cgcaaccccg cgcgacaaac agggagagcg
     5641 catcgcgtag cgtcatctct tctgccagtg cctcaccttc tgcccgttct tcgcgacgca
     5701 cgtaatccgc cacactacgt aacgaaagca gacgcacacc cagttcgcta cgtccaaaaa
     5761 actggcggac aaaatcattc gccggacgag tcagcatcgt cagcggattg ccctgctgca
     5821 ccacttcacc gtgatccatc aataccagat gttcagccag ccgtagcgcc tcatcaatat
     5881 catgagtgac cagcacaata gtgcgcccca gcaaacggtg aatgcgcgtc atctcttgtt
     5941 gcaacgcgcc gcgcgttacc gggtccagtg cgccaaaagg ttcatccatc agtaagactt
     6001 gcggatcggc agccagtgcg cgcgccactc ccacacgttg ctgctgacca ccggaaagct
     6061 gatgcggata acgctcacgc aaatttgact ccagccccag tagcgtcatt aattcgtcga
     6121 tacgatcgtc aatccgtgcc cgcgaccatt tttgtaattg cggcacggta gcgatgtttt
     6181 gcgccacgct ccagtggggg aataggccaa tagattgaat ggcatagccc atccggcggc
     6241 gcaactccag tactggcagc gagcgaattt cttctccggc aaagcggatc tctccgctgt
     6301 catgttccac caggcggtta atcattttca aggtggtgga tttgccggag ccagatgtgc
     6361 caatcagcac cgaaaaactc ccttcctgaa aattgagatt aagatcgtta acggcttttt
     6421 gtgcgccaaa cagtttgctg acatggctaa attcaatcat tacgtttcac cttcagcagt
     6481 gcgataagca aatcgaacag ggcatcgatc agcaccgcca gaacaattac cgggatcacc
     6541 cccagcaaca ctaaatcaat ggcgctgctt agcagcccct ggaaaaccag cgcaccaaaa
     6601 ccgcctgcgc cgattaacgc cgcaatcacc gccataccta cagtttgcac catcaccacc
     6661 cgcaggctgc gcagaaatac cggtaacgcc agtggtaact gaacatgcag gaatcgctgc
     6721 gccccgctca tgcccatcgc tctggcgctc tccagcacat cgcgcgggat ctggttcaaa
     6781 ccgactacca cgccgcgcac cagcggcagc aaggcataga gcaccagcgc aatcagtgcg
     6841 ggtgtcattc cggttcctgc tatgccgagc ttccccagcc acggaaaggc cgtcaccagc
     6901 gcggcaagcg gcgcaatcaa caggccaaag agcgccaccg aaggcacggt ctgaatgaca
     6961 ttgagcagag aaaaaattgc cccctgccga gcagtggaaa agtagcacca gatgcccaac
     7021 ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca gcaacgtcag atgttgtgcc
     7081 agcgcgtcgt caaacacatc ctgacggttg gcgtattctt tcattagtga gagatcgtta
     7141 agcgtgccgg agtacagcaa ccacagcgga ataatggcaa tctgcatatg caacaaccag
     7201 cgccacagcg gatgcgtgga gattcggcgg atggcatcgc tacaggccag caatgccagc
     7261 gccgcagcca gccagaaacc actgccgagg ctggtacgcg ccagcgcact gccattttgc
     7321 gccagttgtg tcgccgcctt tcccgcgccc cacaccagca atacgaagac gaattgcgcc
     7381 agaatgagtg cacaaatgct gcctttttta ccgggaataa aacaggccgt cagccaggcg
     7441 caaccaacgc ccaccagcat ccagatcgtt tgcggccaca gttgccagag atgacgccct
     7501 tcacccgaga ctaaacgatt aggcgcgtag ctgataaacg gcagcgctgc tgcgattgcc
     7561 gtcagcaaca gcagcagcgc cagaacagga ttaatacgta gataagtcac gggaaattac
     7621 ttcgcccacc ctttttgttt caggtagtcg gcagccactt ttttggcatc cagtccttcc
     7681 actgcaatgc tggcattcag ttgctgcaat gtttttgcat cgaggctggc gaagactggc
     7741 tgtagccact gtgccatttg cggatactcc ttcaacaccg attcacgcac cactggtgca
     7801 ggcgcataga taggttgcac gccttgcgga tcgcttaagg tttgcagccc cagcgccgct
     7861 accgagccgt cagtgccgta agccattgca gcattaacgc cagaggtttg ctgggcagcg
     7921 gctttgatcg tcaccgccgt gtcgccacca gccagtgaca gcaactgatc ctgaccgagc
     7981 ttaaagccat aggctttttc aaacgcgggt aacgcatcgg cgcgttcgat aaactctgcc
     8041 gaggctgcca gtttgaaggt gccgccctct tgcagataac gactcaggtc ggcaagcgaa
     8101 gtgagtttat ttttctctgc cacatcctga cgcacggcga tagtccaggt gttatttgca
     8161 ggcgcgggcg tcagccagat taacttgttg tgctccgaat cgagcttttt gactttctcg
     8221 taaccttgct gcgcgttttt ccacgctgca tcgttttcat ctttaaagaa gaaagcgcca
     8281 ttgccggtat attccggata gatatccagt tcaccggaag taatcgcccc gcgcaccaca
     8341 ggagtcgttc cgagttgcac tttattcacc gtcggtacgc cgtggctttc cagcacctgc
     8401 aaaatgatat tgccgagtag cgcaccttcg gtatcgattt ttgaaccgac tttaacgggg
     8461 gaagccgcct gtagcggcag gctcacggct gccaacataa ccagtgaacc tgcccagagc
     8521 tttaagagtg gcatgatgct ttcctcattc ttttactgtt gttttcagcg aattaagaga
     8581 aaagcatagt tgataatggc ggggttagct tgagcggaag gattcagttg cagaatcaga
     8641 taaatactta agaggcatat tcggtccggc gtttcctgcc ggatgcggcg cgagcgcctt
     8701 atccggccta caaagggcgc aaacgtcgcg cccttactaa agcattacag caactcaaac
     8761 tcgcctttct taacgcgtgc ggaatcagtg ccgataaata cattgaactt gccaggctcg
     8821 gcgtcatatt tcatctgttg attccagaac ttcagcgcct caatatcgat cgggaagctg
     8881 acagtctgag tttcgcccgg tttcagggtg attttctcaa agcctttcag ctgtttcaca
     8941 gggcgactca tggaagccgt cacatcctgc aagtacatct gcactaccgt ggccccctcg
     9001 cgcttaccgg tgttcgtcac ctgcacgctg gcagtcactt tgccgtcacg cttcatggtc
     9061 ggcgcagaaa gtttcacatc agagacggtg aaagtggtgt agctcagccc atagccgaac
     9121 ggatacaacg ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt cggcttgtcg
     9181 gcattatacg ggcgaccggt attcagatgg ctgtagtaca ccgggatctg cccgacagaa
     9241 cgcgggaagg acattggcag cttgccggac gggttgtaat cgccaaacaa tacatcggca
     9301 attgcattac cgccttcagt ccccgcaaac caggtttcca gaatcgcatc agcctgctga
     9361 tcttctttca ccagcgccag cggacgcccg ttcatcagca ccagcaccag cggtttaccg
     9421 gtggctttca gcgccgcaat caagtcacgt tggctttgcg gaatagtgat atcggtccgg
     9481 ctggaggctt cgtgcgccat cccctgtgct tcaccgacta cagccaccac cacatcagat
     9541 tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg gcgaacgcgg atcgactttg
     9601 accgcttctt catactgatt caggaaatcg ataatgcctt tgtcactggt aacgttcgcc
     9661 cctttggcat acagcacttt accgttttca ccgacggaat ttttaatccc ggtcagtacg
     9721 gtcacggatt gatcggcaac accggctgcg gaccagctgc ccatcacgtc acgtttactg
     9781 tccgccagtg gcccaaccac cgcaatggtg gccgattttt tcagcggtaa cgtttcgaga
     9841 cggtttttca gcaacaccag gctttcgcgc gccacttcac gcgcttcttt acggtgcagg
     9901 cggctttcgg cattggtatc caccgggtca gactctttcg gccccaaatg gctgtatggg
     9961 tcgttaaaca atcccatatc atatttaacg ttcagtacat ggcgggcagc atcgtccagc
    10021 tcttccatcg tcactttgcc ggatttgatc aacccaggca gatacttcga gtaatactcg
    10081 tcgctcatac tcatgttgat tccggatttc agcgccacgc gcaccgcatc ttccgggtct
    10141 gccgccgtgc catgtttaat cagctctttg attgcaccgt gatcggaaac ggtgatgcct
    10201 ttaaagcccc actggtcgcg cagaacatct ttcagcagcc aggaatcgga ggtggctggc
    10261 gtgccgttca gcgagttcag cgccaccatc accgcgccgc tgcctgcgtc cagccccgct
    10321 ttgtacggcg gcatataatc attaaacagg cgctgcggac tcatatcgac ggtgttgtac
    10381 tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt catcaccgag
    10441 tagcgatctg ccgggctttt accctgcatc gcttccacca tggttttacc cattgttgag
    10501 gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg atcgcgcgag
    10561 acatcgacca tcggtgccca ggtcatattc aggccatcat ctgccgcttc ataagcagag
    10621 acacgtccga ccgttttcac tgcatcgagg ttaaaagacg aggccagacc gaggctaatc
    10681 gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg aattttcagg
    10741 cggcttaatt ccatcacctg atcctgcatg gcgcggatat cctgacgggt tacggtgttg
    10801 aaaatcgccc caacctgacc gtctttgatc atctcgcgga tcgcctcttt cgggttatcc
    10861 gggccgacgc tgattaagcg cagctgacca attttctcat caactgtcat tttcttaagc
    10921 agttcggtga cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc gaataaatca
    10981 tccgccagtg ctggctgcag ggccagactc accgcgattc ctactgaaca tagccatttc
    11041 atatggattt ttcttcctct gttgcccgac gaggcagcga aaatggtgca aaaaccgtag
    11101 tttgccataa gcatgatgga gagagaaaaa gaatgctcag tttattgtct gaattttcaa
    11161 aatattcact cgctgaattg tcatacaagg cgctattcta gtttgtgata ttttttcgcc
    11221 accacaagga gtggaaaatg tcttccatga caacaactga taataaagcc tttttgaatg
    11281 aacttgcccg tctggtcggt cattcacacc tgctcaccga tcccgcaaaa acggcccgct
    11341 atcgcaaggg cttccgttct ggtcagggcg acgcgctggc tgtcgttttc cctggctcac
    11401 tactggaatt gtggcgggtg ctgaaagcct gtgtcaccgc cgacaaaatt attctgatgc
    11461 aggctgccaa tacaggcctg accgaaggat cgacgccaaa cggtaacgac tatgatcgcg
    11521 atatcgtgat catcagcacc ctgcgtctcg acaagctgca cgttcttggc aagggcgaac
    11581 aggtgctggc ctatccgggc accacgctct attcgctgga aaaagccctc aaaccgctgg
    11641 gacgcgaacc gcactcggtg attggatcgt cgtgtatagg cgcatcggtt atcggcggta
    11701 tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc gaaatgtcgt
    11761 tattcgcgcg tataaatgaa gacggcaaac tgacgctggt gaaccatctg gggattgatc
    11821 tgggcgaaac gccggagcag atccttagca agctggatga tgatcgcatc aaagatgacg
    11881 atgtgcgtca cgatggtcgt cacgcccacg attatgacta tgtccaccgc gttcgtgata
    11941 ttgaagccga cacgcccgca cgttataacg ccgatccgga tcggttattt gaatcttctg
    12001 gttgcgccgg gaagctggcc gtctttgcgg tacgtctaga taccttcgaa gcggaaaaaa
    12061 atcagcaggt gttttatatc ggcaccaacc agccggaagt gctgaccgaa atccgccgtc
    12121 atattctggc taacttcgaa aatctgccgg ttgccgggga atatatgcac cgggatatct
    12181 acgatattgc ggaaaaatac ggcaaagaca ccttcctgat gattgataag ttaggcaccg
    12241 acaagatgcc gttcttcttt aatctcaagg gacgcaccga tgcgatgctg gagaaagtga
    12301 aattcttccg tccgcatttt actgaccgtg cgatgcaaaa attcggtcac ctgttcccca
    12361 gccatttacc gccgcgcatg aaaaactggc gcgataaata cgagcatcat ctgctgttaa
    12421 aaatggcggg cgatggcgtg ggcgaagcca aatcgtggtt ggtggattat ttcaaacagg
    12481 ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag caaagcgttt ttacaccgtt
    12541 tcgccgctgc gggcgcagca attcgttatc aggcggtgca ttccgatgaa gtcgaagaca
    12601 ttctggcgtt ggatatcgct ctgcggcgta acgacaccga gtggtatgag catttaccgc
    12661 cggagatcga cagccagctg gtgcacaagc tctattacgg ccattttatg tgctatgtct
    12721 tccatcagga ttacatagtg aaaaaaggcg tggatgtgca tgcgttaaaa gaacagatgc
    12781 tggaactgct acagcagcgc ggcgcgcaat accctgccga gcataacgtc ggtcatttgt
    12841 ataaagcacc ggagacgttg cagaagttct atcgcgagaa cgatccgacc aacagcatga
    12901 atccggggat cggtaaaacc agtaaacgga aaaactggca ggaagtggag taaaaattac
    12961 ggatggcaga gtatcgccat ccgaattcac ttaatcgttc tgtgccgtct gccccgccgc
    13021 cgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggca caggcatcac
    13081 tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc
    13141 aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtac gcatcaccag
    13201 acaatggccc gccgcattgg taaagccggt tttggttaac tgaatattcc agttatcgcg
    13261 atacaccaga tgattagtat tgcggaacgg cagcgtatac gtcgggttag agaaggttgc
    13321 catatcttcc cgggtagtac ttaactgccc gatcaacgga tactgtttgc tggcaatgag
    13381 cagtttggtt aagtcacggg cagttgaaac gttatgcacc gacaatccgg taggttcaac
    13441 aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcattgcct taataaaggc
    13501 tttgtaacca ccgggataat ggtgcgcaag gcttgccgcc gcgcggtttt ctgaagacat
    13561 cagcgccagc aacagcatat ctttacggct gatttcgcta ttcagtcgta cgcgcgaata
    13621 gacccctttc atctccggcg tctggctgat atccactttt agtttttcat ccagcggcag
    13681 tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa tcggacgcac
    13741 cagatccggg tggttcgaat agatcacttt gttggtattc agatcaacaa tcatcgcgct
    13801 accggaggca atttccggtt gtgaagcggt ggtagcggct gccgttttcg caacggcctg
    13861 cggtgccaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc gaaatttcgg
    13921 catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg gagtaaggat
    13981 ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa aggagaatcg
    14041 tgaggaaatg ctgcattgct gacatttacg ccagcaatgc aatgtcaaag aactttctag
    14101 aacaggcgat acccgtagcc ccatagtata acggttaggg cgagcagcac ttccagtacc
    14161 agcacgccaa tcgccagcgt cgaactggag aagctaaggc cttcctcttt gttgatattc
    14221 aggaagctcg gaataccaag gtaaagcagg tagccggtgt aaaacagcgc caccgtgccg
    14281 accagcgcac acaaccagac cagcggataa agcgccacca gaccgcttaa aaacagcgga
    14341 gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg cgggtaattg
    14401 cgcgccatcc accagatgac ccgccccatc accgcaaccc cagcgagcat aacgccataa
    14461 aacaggacag ccagcgccag tccggtaaac caggacaact tcaggatagt gccatcgcca
    14521 aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc
    14581 agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt tttcgcgatt aatcacctgc
    14641 atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc cccttgttgt
    14701 gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt tgttcgctca
    14761 tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg aatgatttcc agggtgtatg
    14821 cttacaggca accaaaggga gacagactgg cctatggatc tcaatacact catctcacaa
    14881 tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt gactttgctg
    14941 ggaggcgttg cggcgcatca ggggctatta aagttcccgc tggtggtact ttctgtggcg
    15001 cttggcggca tgattggcga ccaggtgctt tatctgtgcg ggcggcggtt tggcggcaag
    15061 ctgttacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa acttatccaa
    15121 cgccatccgt atctgtttgt cattggtacg cgctttatgt atggctttcg ggtgattggc
    15181 ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccgct taatattctc
    15241 ggcgcatttg cctgggcgtt gatttttacc actatcggtt acgctggtgg tcaggtgatt
    15301 gcgccgtggt tgcacaatct cgatcagcat ttgaagcact gggtctggtt gattctggtc
    15361 gtggttctgg tggtaggcgt gcgctggtgg ctgaaacgac gcgggaagaa aaagccggat
    15421 aatcaggcgt aaaaccattg ccctggataa ggcgttcgcg ccgcatccga catccggtgc
    15481 accatgcctg atgcggcgct tgtgcgtctt atcatgccta ccaaaatttt acccattgaa
    15541 atatccacac acctcactgc gctattctgg gttgaactgc ggattcgcca acataaagcc
    15601 gccatccact atcaacgact gcccggtggt gtaatttgcg ccctccgaac aaagccacgc
    15661 caccaggctg gcaatctcat acgttgcgcc aaaacgccgc agaggaatcg aaggctccgc
    15721 gtcgggcttc acgtcgctat catccatgcc gttcattggc gtagcgatcg ccccaggtgc
    15781 gactgcgttc accaaaatct tatgcctgac caactccagc gccattgctt tggttaaccc
    15841 gccgagcgca tgtttagcgg ctgtgtaggc gctggcatcc ggcagcggcg tatgttcatg
    15901 taccgacgta atgttgatga tgcgaccacc ctgcccttgt ttcaccatct ggcgggccgc
    15961 aatttgtgag cataaaaatg caccatcgac atcaacggta aaaatcttgc gccactcatc
    16021 gaaagccata tcaagaaacg gcgctttggt cattgcgccc gcattattca ccagcacatc
    16081 aatacgcccc agccgttgaa tgagtttctc cagcgcctgt gcaccttctg gcagtttacc
    16141 gagatccagc tgcacaatct ccgcacgtac gccgtggcta actacctcac gcgcggtatc
    16201 ttttgcccct tcttcatctg agtgccaggt aatgccaata tcaaacccct gctgcgccag
    16261 taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg caacctgtgc
    16321 catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca tatcatcagc
    16381 gtttgtaacc cccgcccaac gcaccagtga gttgaatgga agcatccagc cactgccctt
    16441 gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg ctgacgcggg
    16501 aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag acctaccaca cgcaaggcat
    16561 cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag tgtctgaacc tgactggctg
    16621 cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggcg atagacaagt
    16681 tgctttcggc ttttgcgata tcgagattgg cgttaagacg accactatcg aaaatgggta
    16741 gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg acggaacaga tcgcttaagt
    16801 gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc aggataaaat gccgcttttg
    16861 ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc
    16921 gccgggccag taaggagtac cccagttcat caggaagctg gcttgccact ttcggcaacg
    16981 cgaccggttt aagcttcaat gactttgtct ggttatttgt aagtgcgctt aaccgtgcct
    17041 caataatttt cattttcccc gcgacatcgt tgagctgctg ccgggttttg ctggcattaa
    17101 tatcggtttc cacaccttca actgaagaag taatcccgtt ctgatatagc tggcgatcgg
    17161 tcgcgataat ggtgttctgc tctttttcta tttgctgcaa gaccgtgttt aacgccgcct
    17221 gggtttgcca ctcccagtac aggcgggcta cgctgccagc cagcaattgg cgggtttgct
    17281 cgcgttccgc cgcccgtgct ttaaccgtac ccaggcgggc agtaacctcc gcccgattct
    17341 ttccccagat atcgagatgc cagcccgccg ttaagccaaa agttccgttg gtgtaccacg
    17401 gaccggtcgt acctgcggcc ggatcgttca gagcaaacgg ccccattaag ccttctgccg
    17461 acattttttg ccgctccata tccgcagaaa agtcgatctg cggaccatcc tgagtagcaa
    17521 ttgccttcgc ctgggcttca gctagctgaa tgcgctgttc agccacctgc atatccggtg
    17581 cgttctgtag tgcattgtta attaaggaag tgagttgatt atcgtgatac tccagccacc
    17641 attggctgtc tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca acttgtgcag
    17701 cgggcgtttg ctggcttaac gcctggcggg tttcatgcat aggcgcacac ccggccagca
    17761 tcagtaacag cggaaaacag gcgatggcgg gataaaagga atcacgattc atgggggaat
    17821 aatcaggtaa gaaaaggtgc gcggagatta ccgtgtgttg cgatatattt tttagtttcg
    17881 cgtggcaata catcagtagc aataaaacga catatccaga aaaatataca ctaagtgaat
    17941 gatatcttcc gatttatctt aatcgtttat ggataacggc aaagggcttc gttttttcct
    18001 atacttattc agcactcaca aataaaggaa cgccaatgaa aattatactc tgggctgtat
    18061 tgattatttt cctgattggg ctactggtgg tgactggcgt atttaagatg atattttaaa
    18121 aatcagatat ggcatataag ttgtcaggtc aggaactaga tattttgtgt acttgatttg
    18181 caaacagctc cggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccggggca
    18241 gcccacatgg atgtgggctg agggcgcgtc ttacaaggac gttacctcgc gcccgacccg
    18301 gtagacgaag gggataagct gaaggtaccg cgcagcggcg attttgttcg ccagagcccg
    18361 ggggtgcatg gggcggcggc gattggccgc cccctgcgcg ctccttgcgc cagtggcaat
    18421 atgtggctta gctcatgaaa ggagcgcaac aagatgatga agctgaataa gcgattcatt
    18481 gtctctgata aactgcgatt ccactattat cgcagcttat cgatatcaat tgcctgaata
    18541 gcccttgcaa tatcagggga attattcaac acccgaacat gctgaaataa ttccgttgct
    18601 tcatcgtatt ctttacgcaa ataactcaac cactgtttaa tccgcgcaac gtgatataac
    18661 ccggtatcgc cctgcttttc cagacgggta tatttttgca gcaaagcaac cacctccggc
    18721 cacggcattc gcggttcgtt atattttact acccggctca ggttgggaat attgagcgcc
    18781 ccgcgaccaa tcatcactgc gtcgcagccg ctgatcgcaa tgcattgttg cgcgctctgc
    18841 cagtcccaga tttcgccgtt ggcaatcacc ggaatattca gccgctggcg aatctcgcca
    18901 atcgcctgcc agtcaatatg ctccgcgcgg taaccctgct cttttgtccg tccatgcacc
    18961 accagctccg tagcaccagc ctgttgaacc gcatcggcga tttcaaattt cttctcaccg
    19021 ctgtcccagc ccagacgcac tttcacgctg acgggcaaat gcgccggtac agcttcacgc
    19081 atcgcttttg caccctggta gatgagttca ggatctttga gtaacgtcgc cccaccaccg
    19141 ctaccgttga ccgttttcga cgggcagccg caattgagat ccacgcccca ggaacctaat
    19201 tccaccgcac gggcggcgtt ctctgccagc cattgtggga actgacccaa caactgcacg
    19261 cgcaccagcg taccggatgg tgtccggctg gcgttttgta gctcagggca aatgcgatga
    19321 aaaactttta ccggcagcag ttgatccacc acgcggacaa actcggtgat gcacagatcg
    19381 tagtcgttaa cttcggtcag caattcacgc accagagagt caagcacacc ctccatcgga
    19441 gccagtaaca cacgcatatc ttcacccgca aaaaaatgag gcgctatgtt agcgcctatg
    19501 gtcaacggat taaaggtctg caaagaggga ttgctgcaaa ctgtaatccc tctctatgct
    19561 tgatgtatgc ctgagttcgt tgcgcattgc gtaatgccgg ggagcgaaaa aataagcgct
    19621 gatttcttaa tgtgatcggt agcacgtttt acgaattaat tgtatgatga atccatctca
    19681 tctggggtgt tgattatgag taagacactg aacattatct ggcaatattt acgcgctttc
    19741 gtcctgattt atgcctgcct gtatgcaggc attttcattg cttccctgct accggtaacc
    19801 attccgggca gcatcatcgg tatgctgatc ctgtttgtcc tgctggcctt gcaaattctt
    19861 ccggcaaaat gggtcaatcc ggggtgctac gtactgattc gctatatggc gctattgttt
    19921 gtgccgattg gcgtaggcgt catgcaatat tttgatttgc tccgcgcaca gtttggcccg
    19981 gtagtggttt cctgtgcaat cagtacgctg gtggtttttc tggtggtgag ctggagttcg
    20041 caactggtac acggtgaacg taaagtcgta ggtcagaaag gatcagaaga atgatggcaa
    20101 atatctggtg gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg
    20161 cacggtataa gtttcccctg cttaacccgt tgctggtagc aatggtggtg atcattcctt
    20221 ttttaatgct gaccggcatc tcttacgaca gctactttaa aggtagcgaa gtgctaaacg
    20281 atctgctgca accggcggtc gtcgcgctgg cctatccttt atatgagcag ctacaccaga
    20341 tccgcgcgcg ctggaaatcg atcatcacca tctgttttat cggcagcgtg gttgcgatgg
    20401 tgacggggac ttccgtggca ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc
    20461 tgccgaaatc agtcaccacg cctattgcaa tggcggttgg cggcagtatt ggcggtattc
    20521 cggcaatcag cgcagtttgc gtgattttcg tcggcatcct cggcgcggta tttggccata
    20581 cattgcttaa tgcgatgcgt attcgtacca aagctgcgcg cggtctggca atggggactg
    20641 cctcgcacgc cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta
    20701 gttcgctagc gctggtgtta tgcgggataa ttacttcgct gatcgcaccg ttccttttcc
    20761 cgattattct ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt
    20821 ttcacgcgtt gcataattaa tgagattcag atcacatata aagccataac gggttcgtaa
    20881 actgttatcc cattacatga ttatgaggca acgccatgca tccacgtttt caaaccgctt
    20941 ttgcccaact tgcggataac ttgcaatctg cactggaacc tattctggca gacaagtact
    21001 tccccgcttt gttgaccggg gagcaagtct catcgctgaa gagcgcaacg gggctggacg
    21061 aagacgcgct ggcattcgca ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt
    21121 cgaattttaa tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca
    21181 atatggaatt tattggtgcg acaatgcagc aaaccgttca tgccgaacaa agcgcgatca
    21241 gccacgcctg gttgagtggt gaaaaagcgc ttgcagccat caccgttaac tacacgcctt
    21301 gtggtcactg ccgtcagttt atgaatgaac tgaacagcgg tctggatctg cgtattcatc
    21361 tgccgggccg cgaggcacac gcgctgcgtg actatctgcc ggatgccttt gggccgaaag
    21421 atctggagat taaaacgctg ctgatggacg agcaggatca cggctatgcg ctgactggtg
    21481 atgcgctttc tcaggcggcg attgcagcgg caaaccgttc gcacatgcct tacagtaagt
    21541 cgccaagcgg tgtcgcgctg gaatgtaaag acggtcgtat tttcagtggc agctacgctg
    21601 aaaacgccgc attcaacccg actctgccac cgttgcaggg agcgttaatt ctgttgaatc
    21661 tcaagggtta tgattacccg gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc
    21721 cgttgattca gtgggatgcc acctccgcaa cgctgaaagc tctcggctgt cacagtatcg
    21781 accgagtgct tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg
    21841 ccagccctct cctggtgtcg aaattcccgg caaacagttt gccgtttctt gcgcaaaacc
    21901 agcgggtaaa gtagcctgat ggaaattttc cttagatcga gtctcctgca tgttaaagcg
    21961 cgtgttcctc agcctgttag tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga
    22021 tcgctggatg agctggaaaa ccgcgcctta tatctacgac gaattgcagg atctccccta
    22081 ccgccaggtc ggtgtggtgc tcggaacagc aaaatattat cgtactggcg taattaatca
    22141 gtattatcgc taccgcattc aaggagcgat taatgcctat aacagcggta aggtaaatta
    22201 tctattactg agcggcgata acgcattgca aagttataat gagccgatga ccatgcgcaa
    22261 agatttaatc gctgctggtg tcgacccatc agatattgtt ctcgattacg caggctttcg
    22321 tacgctggat tccatcgtgc gtacacgcaa agttttcgat actaatgatt tcattattat
    22381 cacccaacgt ttccactgtg agcgggcatt atttattgcg ctgcatatgg ggattcaggc
    22441 tcagtgttat gccgtaccgt caccgaaaga tatgctgtca gtacgtattc gcgaatttgc
    22501 cgcccgtttc ggtgcgctgg ctgaccttta tatttttaaa cgtgaaccgc gttttttagg
    22561 gccgctggtc cctattccgg ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc
    22621 cgtcacaccc gaacagttac ttgaattaca aaagaaacaa ggaaagtagt tatggatgta
    22681 cagcagtttt ttgtcgttgc cgtttttttc cttattccga tattttgttt ccgcgaagca
    22741 tggaaaggct ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg
    22801 tatgtctggc gagcaaaaaa tcccggactc tttttcgctt atatggtggc atatatcggc
    22861 ttcggaattt tatctatcgg catgattgtt tatcttattt tctatcgtta atattttctc
    22921 ttttctgtta attattgaga ataattatta cttcactcaa taagctgcgg atataacatt
    22981 ctgaaccgca gctatttgtg aatcctttca cagtataaat ttccccacgc gcatagcctt
    23041 aatatcagca cgcaatattt atactctaaa taattcgagt tgcaggaagg cgacaagtga
    23101 gtgagtcccc aggagcttac ataagtaagt gactggggtg agcgaacgca gacgcagcac
    23161 atgcaacttg aagtatgacg agtatattta gcgttcgcct taattactca tagcattaag
    23221 gaagatcaca tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgccttta
    23281 ctggcgatta aagaagcctc tcgctgttta ttatgtcatg acgctccctg tagtcaggct
    23341 tgcccggcac agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa
    23401 ggcgctgccg agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc
    23461 ccgacggaga aattatgtca aagcggttgt acccgtgccg gtgttgatac tcccattgat
    23521 atcggtcgct tacagcgttt tgttactgat tttgaacaac aaaccgggat ggaaatttat
    23581 cagcccggta ctaaaacgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg
    23641 caggccagtg taacactgac aaaccagggt tatgacgtca cgatttatga gaaagaagcg
    23701 caccccggtg gctggttgcg taacggtatt ccgcaattcc ggttaccgca atcagtgctg
    23761 gatgcagaga tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc
    23821 ggtaacacac tcacccttga gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg
    23881 gggttatcga gcggttccgg gttatcgctg tttgagcata gcgacgttga gattgccgtc
    23941 gacttcctgc aacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta
    24001 attatcggcg gcggtgatgt tgcgatggac gtagccagca cgctgaaagt tctcggctgt
    24061 caggcggtaa cctgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag
    24121 tttgccagcg cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg
    24181 gaaggcaata aagtcacgtt taagcatgta cggttaccgg gcgaactgac gatggcggca
    24241 gataaaatta ttctcgccgt cggtcagcat gccagactgg atgcctttgc ggagttagag
    24301 ccgcagcgta acaccatcaa aacacaaaat tatcagaccc gcgatccgca agtctttgct
    24361 gctggcgata ttgttgaggg tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa
    24421 gccgccgagg cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt
    24481 cgattacttt ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgcctgtag
    24541 gcaactgcta tgagatgtgt gccaaagcct acgacacagg ttggggcggc gtggtgttta
    24601 aaacgatcgg cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag
    24661 aagataccgg ttttatcggc ttcaaaaata tggagcagat tgctgaacat ccgttggaag
    24721 agaatctggc cgccctgcgt cagctgaagg aagattaccc ggacaaagta ttgatcgctt
    24781 cgatcatggg ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg
    24841 gcgcggatat gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta
    24901 gcgatgtcgg gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt
    24961 ccacgctgcc aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc
    25021 tggcggcgaa gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca
    25081 ccaatatcga tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcgagtattt
    25141 ccggatattc cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag cagatgcgca
    25201 cccatccaga actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg
    25261 atgcggctga gtttttattg ctcggcgcag caacgttaca ggtgaccacc ggcatcatgc
    25321 agtacgggta tcggatagtg gaagatatgg caagcgggtt gtcgcattat ctcgccgatc
    25381 agggatttga ttcgctgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg
    25441 aagatttaga ccgcagttat attgtctatc cccgtatcaa tcttgataaa tgtgttggct
    25501 gtggacgctg ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga
    25561 aaacccgcac accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg
    25621 tctgcccggt gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac
    25681 acccggtaac gttgtaaaac cagagacgca tccggcattt ggcaccgatt gccggatgca
    25741 gcgcatccgg caatatccac aatcacatca cttcttacgc gcgtatttca gtgaatccag
    25801 cgctacggcg aagataataa tcgccccttt gatgatgtac tgccagtatg ggtttacgcc
    25861 gatatacgtc aggccatagt tgatgacggt aaaaataatt accccggtca ccacgccaat
    25921 caccgtcccc acaccgccgc tgaacgatac accgcctacc acgcacgccg cgatagcatc
    25981 cagctcatac ataaagccga ggttgttggt ggcagagccg atacgtccgg cttctaacat
    26041 cccgccaaag gcatagaaca cgccagacaa cgcgtagatc atcagcaggt tcaggccgac
    26101 gttgacacca gatacttttg ccgcttccgg gttaccgcca atggcaaaaa tgttcttacc
    26161 gaagcgggtt ttgttccaca acacccagac gaacgccacc gcaatcaacg cgtagaaggt
    26221 gatgtaagag agacggaaac tccccagcac gacaaagccc tgagcaaagg tagagaagcc
    26281 actgtcaaaa ccagaaattg gcgacgcccc gacaaagtcg taatagagcg agttgattcc
    26341 atagacgatg atcatcgtgc cgagcgtggt aatgaacggc gtcacgttga gataagcgat
    26401 aatcagaccg ttgatcaaac cgatcaccgc accaatggca cagacaatca gaataaccag
    26461 cgcaatcggc atcgtcgcca tttccgggaa cactttgttg gcgttatcca tggactgcaa
    26521 taatgtcgca gccaccactg ccgccagccc tacctgacga ccagcagaaa gatcggtccc
    26581 ctgggtgaca attaaccctg ccacaccgag cgcgataata atacgcaccg atgactgggt
    26641 gagaatatta cttaagttca acagacttaa aaatgttggg tcctggaaaa taataatcgc
    26701 cagcaaaact aaaagaacga cgtaaatacc gccctctttc aggtaagtaa gaaaactttt
    26761 cttatttaac gcactcatgg gaagccccta atcttaaagg tgcaaagacg caagacgcag
    26821 aatttcgttt tgcgttgttg tttttgtatc gacaattccg gaaacgagac cattgctcat
    26881 gaccagaata cggtctgtta tccctaacaa ctcaggcatt tcagaggaga taataataat
    26941 ccccttgcct ttcttcgcca gttcggcaat taactgataa atttcaaact tcgccccgac
    27001 atcaataccg cgcgtcggtt catcgagcat taatatttct ggttgcgtta gtagccagcg
    27061 accaataatc accttttgct gattaccacc ggagagcgaa ccaatttgcg tccgatgacc
    27121 cggcgttttt acccgcatcg aatcaatcac ccactgggta tcgcttttca tccgcgagtt
    27181 atccagtaaa ccaactttat ttttgtagtt gcgaatattg gaaattaacg agttaaaact
    27241 aatatccaga taggcataaa ttcccgttga gcggcgctcc tcagttacca gtgcaaatcc
    27301 gtggtttatg gcttcgttgg cattatggtt attgatctgt ttgccgtgca acgtaatggt
    27361 gccagccgat ttctcgcgaa taccaaataa cgtctcaaca atatcggtac gtttcgcccc
    27421 caccagcccg gcaataccga ggatctcccc tttatgcaga tcaaacgaga catcgcgaat
    27481 cgacggctgg cgcagtgacg ttaggttacg cacctcgagg atgacttcgc ccggcttgtt
    27541 ttctttgtca ggaaaacgct gattaagaga acgcccaacc atcatggcga tgatcttgtc
    27601 catcgtcagt cctgccagcg gttcggtggc gatccactga ccgtcgcgca atacggtaac
    27661 ttcatcacat aactggaaga tttcttccat tttatgcgag atataaacaa taccgcagcc
    27721 gcgctctttt aatttacgaa taatagtgaa cagatgattg acctcttttt cggttaacga
    27781 agaagtcggt tcatccataa tcacaatttt cgcgttatag gaaaacgctt tggcgatttc
    27841 gatcatctgc atttgcgaaa cggataatgt gccgacgcgc gcacgcggat cgatatcaat
    27901 atccagttca tcaaaaatcg ctttggtttc gcggtacatt ttgtcctgat cgacaaacat
    27961 gcctttggtg ggatatcgcc ccagccacat gttatccatc accgaacgtt gtaataccag
    28021 gtttaactcc tggtgtacca tcgaaatacc attttccagt gcctctttgg cggaatggaa
    28081 atcgatctct ttaccctgga ataaaatggt gccggagtct ttttgataaa taccaaacag
    28141 gcattttaat aatgtcgatt ttcctgcacc gttttccccc attaatgcat ggatagaatg
    28201 tggccggact tttaaattaa cgttatcaag tgccttaaca ccaggaaagg acttgttgat
    28261 accgctcatt tccaacaagt attccccgga agacggagtc gttgagctga ccatataatt
    28321 ttaccttgtt ggccatacaa taagggcgca gtaaacgact gcgcccaatc agtcttattt
    28381 cttgctgaat tcagccaggt tgtctttatc tacgccaaca taaggtacgc ggaccacttt
    28441 gttgtcgatt ttccagttgg tgccatcagc cgcacctttg ccatcggcca ggtttttcgc
    28501 cagatcaaag gtcgctttcg cctggttgtt agcatcgttc agtactgtgc ccgccagtgc
    28561 accggatttc accagcgcca gcgcttctgg cagcgcatcg acgccaaaca ccggaatgct
    28621 ggacttgttg tgtgctttca gtgcttctac cgcgcccatt gccatcgcat cgttgttggc
    28681 gataaccact tcgattttgt tggcgttcgg gccagacagc caggcgtcca tcttatcttt
    28741 cgcctgagcg gtatcccaca tagcggtatc taactgtaac tgttcagttt taatgccttt
    28801 gtcgttcagc tctttaatca cgtaagtggt acgtgcttct gcatccggat ggcccggttc
    28861 acctttcagc aatacgaatt gaatctgacc gtctttgttc agatcccaac cctgattcgc
    28921 cgcccagtgt ttagcaatca aatcgccctg aataatgccg gactctttgg aatcagtgcc
    28981 aacgtagtag gctttgtcgt agctatccag cgccttacga gacggttctt tgttgaagaa
    29041 aaccaccggc acgttttgcc cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc
    29101 aaccaggttg attgccagtg ccttcacccc tttcgccagc aatacgtcga tctgatcgtt
    29161 ctgcttggac tggtcattct gagaatcatt catcagcagc tgaacatctg gcgcggcttt
    29221 cgcatcttgc tcaatagcct tgcgcactac agacataaag ttatcgtcgt acttatagat
    29281 tgttacacca atgcgagtat cagcagcgtg tgcagcggca ccgaataaca tgctggccat
    29341 cacagcagac agggttaaca ccttcttatt catggtatct ccggtttttc ttatgcaggg
    29401 tagtgcttga gataaatgct cggcggggca gtagagttaa tgaagtgtta ctgaacgccg
    29461 aagctcactt tttaaaattc gttcttccat gcccggtaac gctccagaaa acggctttaa
    29521 ttgttgttat gacgctatta cctcggtgaa agtgattatt cactgttaca tacgggttac
    29581 aacgttaaaa cggtgcaatc atagctatca cattgttaag atactgtgaa atcactcaca
    29641 gattgaaagc ggttacatcg cctgatttgt tgagttagtg atcgtcgccg cattctggcg
    29701 tgttgcgaca gaatggcgac gcactaacgt cggcataaaa cagtggctag cacgaggatc
    29761 aatattgcct gctgccccct gcaaggccag ttcggtggct aatttcgcca ttgaagcaat
    29821 gggataacgc acggtcgtta attgcgggtc ggtgtaacgg gcgatgggaa tatcatcgaa
    29881 accgatgatt gagagatgta acggaatcgc aatgccatta tcttttaatg ctgtcagtgc
    29941 gccagcagcc atattgtcgt tataagcaaa tacagcggta agttgtagat tgcgccccag
    30001 cagttcaacc atcgccgcct caccgcccgg catgtccggc gtaccagtgc caatccagct
    30061 ttccggcgga ataatatcct gctctttcaa cgcactcatc cagcctgctt tacgcatggc
    30121 gtcatcttca atgccgtgac tggaagaaag ataaccaata cgttgatgac cgttattcag
    30181 caacatgcgc gtcgccattc gggcaccgct gagattatcc aggcaaacgc aacgatgggc
    30241 gtaccccggc acaacgcggt tgattaacac cataccggga atgttatcca taaattgcgc
    30301 cagttcatcg tcactcaatg cttttgagtg aacaatcaac gcattacaac gctggcgaat
    30361 taacacctca atggcgtgac gctctttttc cgcttcatga tagctattgc cgattagcac
    30421 gtatttctga tgctgctgag cgaccagatc caccgctttt accagcgcgc cgaaaaacgc
    30481 atcagaaacg tccatcacca ccacgccaat ggtgtcgcta acctgagttg ccagcgcctg
    30541 ggcattggcg tttggccgat aatccagctc actcacggct ttcattactg cttcacgcgt
    30601 gtcggcactg accagcgtgc tgttattgag cacccgggaa accgttgcca cagagacgcc
    30661 agcctgacgc gctacatcac gaatggtgat catattcact acctgttatg agattgcagt
    30721 aaatgactgc ttgctggcgg ctattttgtc agcactgaat acaggacttc gtgaatcgag
    30781 tcacagcaat ggaaacggtt acagccgttt tgttaacgaa tgtgatccag atcgttatct
    30841 tgatgtttta gatattgccg ttccggcagc acgcaaagtt aactgacgcc agagccattc
    30901 caccggcccc tggcggaagt aacacagcca gataacagag aagaggatat tcgccagcca
    30961 taccggaata acaaacgcca gcagctccag gcggtcaaaa tgcataaaca aaccgaggtg
    31021 gtaaaaaagc atggtacaaa tcagcgtttg caatagatag ttggtcagcg ccatccgtcc
    31081 gacgcaggcg atcgcaagca ccagcttaaa gcggctcaat tgcggccaga agccataaaa
    31141 cagcgacgca tagccaatcg cctgaaacgg cgcactcagt tcccgcggca tttgaagtaa
    31201 gaaggcacac cagcgatacg cccagtccag ctgccattgc agggcgatgg caggaaggtt
    31261 aatggtcacc ccaatcgcca ccagcacaaa accagtacga cgatagtgac gtaagctgaa
    31321 ctgccctttc agccagccgc tgcgcatcag cgctgcacca atgagcatca tcccagccag
    31381 ttgccagcca tactgtgcgc ccagtgccag taaactgttg ccaacaccat cggcacgatt
    31441 actgatcgct tcaacgccgc cgtgaagctt ccagtatttt tcatacaaaa tagccgatgc
    31501 atccggcgtc caggcgcggc tggtctggct gtcggaaatc agccccaata acagcaaaac
    31561 gccaagcccc accagataaa gcatgacgcc tgtattaaac aggcttttta ccgatggcgc
    31621 atcgcgcacc agccaccagc agattaagcc caccagcccg taagccagca gaatatcgcc
    31681 gtcccagaac aataaaccgt gaataaagcc caataagact aacagcgtta accgcgactg
    31741 gatccagcgt ctgccacggg gcagcaacat ttgcagtcct gcaccaaaca gcagcgcaaa
    31801 aagcgtgagg aatttcacct gggcgatgag atcaagaaat gcccaggtcc atgcatcctg
    31861 cggcgtaata gcaccgtacc aggcgggatt gagataagcc gcctttggta gcccaaaggc
    31921 gctgatgttt aatagcagga tccccagaat ggcgacgccg cgaacaaaat cgagcgtgac
    31981 gttgcgctcc atggttcctg ccttttaatc agttgtgatg acgcacagcg cgcagaaact
    32041 cgtggcgcgt attctgactg gatttgaaca atccaccaag agaggtcgtt gttgtggcac
    32101 tggttgcatc gcggatgcca cgcgccttca cgcagtaatg caccgcgtcg atcgagacag
    32161 ccacgttatt ggtgcccagc agcgtttgta gcgcaataag aatttgctgc gtcagacgtt
    32221 cctgcacctg cggacgctgg gcaaagaact gcacgatacg gttaattttt gacagaccga
    32281 tcaccgaatc tttcgggata taagccaccg tcgctttgcc atcgatggta acaaaatggt
    32341 gttcacaggt actggtcaga gtgatatcgc gcacggtgac catttcatcg accttcattt
    32401 tgttttcaat gagggtgatt ttcgggaagt tggcgtaatc cagaccggag aaaatttcat
    32461 cgacatacat tttagcgatg cgatgcggcg tttccatcaa actgtcatca gccaggtcga
    32521 gattcagcag ctgcatgatt tcggtcatat gaccagcaat aaggcttttg cgcgtttcgt
    32581 tatccatttc atgcacgggc gggcgcagcg gtgtttccag tcctcgcgca actaacgctt
    32641 catgaaccag ggccgcttct ttactgagtg atggcattta tgatttctcc tgcaggtgtg
    32701 acgcctccgc cctgcgtggg ggcaaagtta ttaagctgat ttacagcttg attattgtgc
    32761 gtgaggcggc gcacataatc cagtattcac agcgataatt attgtaattg ccgctgcctt
    32821 tcatcagcag atgttaaaac atcgttatgc aaatacggaa gtgaaagtta ctcacagcac
    32881 attgaataaa cggtatgatg aagaaattgc aaacaacaca acaaggagcc acgcatggaa
    32941 atgctcgaag agcaccgctg ttttgaaggc tggcagcaac gctggcgaca cgactccagt
    33001 accttaaact gtccgatgac gttcagtatc tttctcccac cacctcgtga tcacaccccg
    33061 ccaccagtgc tgtactggct ttccggatta acctgcaatg acgagaactt caccatcaag
    33121 gcgggtgccc agcgcgtggc ggcagaactg gggattgtac tggtgatgcc agacaccagc
    33181 ccgcgcggcg aacaggttgc caacgatgat ggctacgatt taggccaggg cgcggggttt
    33241 tatcttaacg ccacgcaacc gccgtgggcg acgcattacc ggatgtatga ttatctgcgc
    33301 gatgaattac cggcgctggt tcagtcgcaa tttaatgtca gcgaccgctg cgccattagc
    33361 ggtcattcaa tgggtggtca cggtgcgctg attatggcgc tgaaaaatcc gggtaaatac
    33421 accagcgttt cggcctttgc gccaattgtg aatccgtgca gcgtcccgtg gggaatcaaa
    33481 gcgtttagca gctatttagg tgaggacaaa aatgcatggc tggaatggga cagttgcgcg
    33541 ctgatgtatg ccagtaacgc gcaggatgcg atcccgacgc ttatcgatca gggcgataat
    33601 gatcagtttc ttgccgacca gttgcaacct gcggtactgg cagaagccgc gcgccagaaa
    33661 gcgtggccga tgacgctgcg tattcagccg ggatatgatc acagttacta cttcatcgcc
    33721 tcttttatag aggatcacct gcgcttccat gcgcagtatt tactgaagtg aaagtccgcc
    33781 cggttcgccg ggcatcttct catcagaagc gataatccac tgccataaag taacgacgtc
    33841 cgtcttcgtt atagctgtag tcgtcacgac tgagatcctt gtcgccaagg ttcagcacgc
    33901 ctgcgcgcag tttgacgtct ttagtcacct gccaggccgc gccggtattc cagatggtat
    33961 aaccgcccgg tgttttagcc gtcgcgctgt cggcgcgttt ctgcccggta tagtgaccag
    34021 aaacatagaa tgaccagtct tccagcgcca gcggtttcca gtccagcgta ccgttagcag
    34081 tatggaacgg cagatcggat agcggtttgt tttcgccgtt gctgacatca cgaccatcgt
    34141 tgtaggtgta gttgatcgac agtttccatt catcgttgaa cggaattttc agttcggttt
    34201 ccacgccctg aatacgagct ttgttaacgt tgtagtagct aaataccggt atgcgccgtc
    34261 cgttagcgcc cgtctcaaaa ccaacaaagt tttggtagcc cggtgcagcg ttgacgtcag
    34321 acgtacggct gatgctgata cgatctttca catcgttacg gaaaacggta acgctggatt
    34381 caaccccttc cagccagcct tcttcaccca tgtagtaaag ccccagctcc caactttcgc
    34441 tggtttctgg tttcagatcc gggctaccca caatcttaca tgcgccacgg caggaattgc
    34501 tcgtccagtc agggctaagt tgcaacagag aaggtgcttt aaatgccgtc gcccagcccc
    34561 ctttcaccgt tacggtgtcg gtggcgttat aaaccaggta ggcacgcgga ctccagtgtt
    34621 caccgtaggt ttcgtgatcg tccatacgca cgccggtcgt cagcgccagc ggctcgaaga
    34681 tccgccattc atcttccaca aacagcgcgt actggctggc agacgttttg gagctggttc
    34741 ccccggtcag gttcaccgca tcgctaagtt tgtcgtgacg ccattcaccg ccaaccgtga
    34801 gaaactgatt aattgccgtc agcggcaacg tgtatttgcc gtcgaccgta ttgctttcgg
    34861 aagttatcgg gctgctgttg ccagggtttt tgttctcgac tttctcaccg tagtatttca
    34921 gttcgctggt gccgtaatcc caacgcccat tatggctgac ggagtagttc tggcgttcca
    34981 ggcggttttt gtccagcgag tcggaatcac gatcctgacg gtcgaaaccg tatccggcag
    35041 taaaatcgtg attttgattc ggtgtccagg caaattcgac attgccgtcg cggctggaga
    35101 atccttcaat acgcggcgtt tctccggtat cggtggtcgt tgagttttgc gggtcatcct
    35161 tttcacgttt tgccaggctg ccgtaagctt tcattcccag cacaccatca attaatggtc
    35221 cactggtaaa gaactgaccg ttataggtat caccgcgatc gcgatgttcc tgaatggtgg
    35281 tatcgacggt aacggtaccc gaccatttct gaccgatttt tttggtgatg atattcacta
    35341 caccgccgag cgcatcggag ccgtatagcg acgacatcgg gccacgaacc acttcaatac
    35401 gttcgatgga atcgaccggg atccagttca gatcgaaatc attgtggcgg aagacggcat
    35461 tgcgggagtt aacgcgttta ccgtcgacga gaatcagggt gtagctgctg tccagaccac
    35521 gaatactaac gcccttacgg ttatcccctt cgttcgtcag ttgtacgcca ggcacttctt
    35581 tgaggacatc cttcagattc tgtaccggtt ttcgctgcag gtcttcctgg gtaatgacgc
    35641 tgatactggc gggtgcatct ttgaggtttt gttcaacgga agatgcagtg acaaccatcg
    35701 tttcgccatc atcatcgacc gctaacacag gccatgcaca agaaatagcg gacaaacaca
    35761 gcccgacccg tacgaaaggg ttcaacctaa acattccata tctccatgag gtaactacga
    35821 aaataaaatg ggttatcgct cacatcttct tcacgtcccc ttgcgtacgg cagcatcgcg
    35881 gtggacttat ttgtatgcag gtgatcatcc cagaaagccg gaacagcctc tgatttgtca
    35941 gccttttttg attgcggcgt aacgataatg caaacgataa caattatcaa tccaaatgtt
    36001 aaattttata tccgcatgat ttgtcaggga aatggaattg aaaaagccct ctcggttgag
    36061 agggcttagc aaggaaggga ggaaacttat ttcttatcgt tctgcgggaa cttcatttcg
    36121 ctgtagcgta cgaagtgagt tcctttaatc agcttgtagc cgaaccaaat aatcaggaac
    36181 agcgggatac caatatacgt tgccgctacg ccgccccagt caatggtatc tttcaggaac
    36241 gcttcgtagt tctggcccaa agtgataatc agacacagaa tgaatgcgaa gatcggcccc
    36301 agtgggaaga aacctgaacg gtacggcaga tcgttaatgt cgtgtccctg caatacgtaa
    36361 ccgcgacgga agcgatagtg gctaatggca atccccagcc aggcgataaa acccgtcatc
    36421 ccggaggtgt tcagcagcca caggtatacc gtctggttgc caaacatgga ggtcaggaag
    36481 cacagaccgg caatcaccgt cgtcgcatac agcgcattac gcggcacgcc accacgcgac
    36541 agtttagcga aaatgcgcgg cgctttaccg tcacacgcca gggtgtacag catacgagta
    36601 gacgcataca taccggagtt acccgctgac agcaccgccg tcagaataac tgcgttcatc
    36661 accgccgccg cagagagcag acccgcgtgc tggaacacca gggtgaacgg actaacgctg
    36721 atgtctttaa catcgttacg cagcaggctc ggatcggtgt acggaataat caggctgata
    36781 atcaggatcg cgaacacata gaacaacagg attcgccaga acacctgacg taccgcgcgt
    36841 ggaatgtttt tcgccggatc ttcggactcg cctgcagcaa taccgatcag ctcggttccc
    36901 tggaaagaga agccgacaat catagctacg ccgatcatcg ccgcaaaacc accagcaaac
    36961 ggcgcttcgc cgattgtcca gttgctccag cccgcaggct gcgcgccttt gaagataccg
    37021 ataatcatca gcacgccaac gatgataaag acaataactg tcgtgacttt gatcagtgag
    37081 aaccagtatt ccgcttcacc aaagccacga actgagatgt agttcagcag gaagataacg
    37141 ccgaggaaca acgcactcca gatccagccc ggtgtatccg ggaaccacca gctcatgacc
    37201 agctgagctg caaccaggtc aacggcgata gtcaccgccc agttgtacca gtagttccag
    37261 cccagcgcga agccaaagcc ttcttcaaca tagttctgac cgtaagtggc aaacgaaccg
    37321 gaaaccggca tatatgcagc tagttcaccg agactggtca tcaggaagta aaccatcagg
    37381 ccaatcagca tatacgagag caatgccccg cccggacctg cctgagaaat cgttgcgcca
    37441 gaggcaacaa aaagacctgt accgatggaa ccgccaatgg caatcatcgt caggtgacgc
    37501 gcctttaatt cacggcgtaa gcccggcgct tctgtggttt tagtttcgga aaccatacaa
    37561 aaatgctatc catcttaaaa atgaggcgcg attgtagcag acgattggca atccttccgg
    37621 cagaaataca cggttataag acaccttcat gatcgcccag ggattataag taaagcagcc
    37681 aatctctttt ctggcgaatg catgctgagg gcgctgcgtc acgaaatcga cacagcacca
    37741 gcatgttctt gtacagcaac ttaacgcggt acattcgcgg gatcgcaata gtccagaaag
    37801 cgccgtagcg cgttggaaag gtgtttttgc cgatgatgta tacgccacaa cgtacgcatc
    37861 aggcgcggga gagggaccgc aacttcactt aatgtgcctg cctgcaattg atctttaatc
    37921 acccgtcgcg acaggcaact aattcccaac ccatgacgca ccgcatgttt gattgcctcg
    37981 gagttaccta attccatcgc catctcaaac ttcggtaaat gtgacagcaa cagataatcg
    38041 acaatctccc gcgtgccgga accgcgttca cgcaggatcc acggcgcagc ggccagctgt
    38101 tctaaggtga ccggaccacg agccaacggc gaagtcggcg cggcgaaaac caccagctcg
    38161 tcttccagcc acggttcaga aatgatttca gtgctgtggc acggtccttc aataaagcca
    38221 atatcaacgc ggaaatccag caccgcttgc atcacgtcct ggctgttccc aacgctaagt
    38281 tcaatcggca actgcggata atcatggcga taacgggcga taactgcagg cagaatgtag
    38341 ttaccgatgg tactactggc atagatacga atcgcgccgt tgtcttcgcg aaacagttgt
    38401 tcgatttcaa ccgcctgttc aagcaatgcc aacgcacgcg gatagagcag ccgcccgtgt
    38461 tcattaacaa ccagtctttt ccccacgcga tcaaacagtt gcacgccaag ctgcccttcc
    38521 aggtcggtca aggctgcgct cactgctgat tgcgacaacg ccagcatcac cgacgcctgg
    38581 gtggttgatc cacttttcaa tacttctgca aaaacttcca actgccggag ggtgatgtgc
    38641 atagtcgctt accacttata aagattaatt ataaatatat aatc
//
LOCUS       DAZKGS010000026        38401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKGS010000026 DAZKGS010000000
VERSION     DAZKGS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            94..951
                     /gene="focA"
                     /locus_tag="ACHOQY_001369"
     CDS             94..951
                     /gene="focA"
                     /locus_tag="ACHOQY_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9677504.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1006..3288
                     /gene="pflB"
                     /locus_tag="ACHOQY_001370"
     CDS             1006..3288
                     /gene="pflB"
                     /locus_tag="ACHOQY_001370"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9677505.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3480..4220
                     /gene="pflA"
                     /locus_tag="ACHOQY_001371"
     CDS             3480..4220
                     /gene="pflA"
                     /locus_tag="ACHOQY_001371"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9677506.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4302..4892)
                     /gene="ycaK"
                     /locus_tag="ACHOQY_001372"
     CDS             complement(4302..4892)
                     /gene="ycaK"
                     /locus_tag="ACHOQY_001372"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFP9677507.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            4992..5900
                     /gene="ycaN"
                     /locus_tag="ACHOQY_001373"
     CDS             4992..5900
                     /gene="ycaN"
                     /locus_tag="ACHOQY_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9677508.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5901..7331)
                     /gene="ycaM"
                     /locus_tag="ACHOQY_001374"
     CDS             complement(5901..7331)
                     /gene="ycaM"
                     /locus_tag="ACHOQY_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9677509.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7540..8688)
                     /gene="ycaD"
                     /locus_tag="ACHOQY_001375"
     CDS             complement(7540..8688)
                     /gene="ycaD"
                     /locus_tag="ACHOQY_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677510.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9003..9629
                     /gene="ycaC"
                     /locus_tag="ACHOQY_001376"
     CDS             9003..9629
                     /gene="ycaC"
                     /locus_tag="ACHOQY_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9677511.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9665..10528)
                     /gene="dmsC"
                     /locus_tag="ACHOQY_001377"
     CDS             complement(9665..10528)
                     /gene="dmsC"
                     /locus_tag="ACHOQY_001377"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9677512.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10530..11147)
                     /gene="dmsB"
                     /locus_tag="ACHOQY_001378"
     CDS             complement(10530..11147)
                     /gene="dmsB"
                     /locus_tag="ACHOQY_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9677513.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11158..13602)
                     /gene="dmsA"
                     /locus_tag="ACHOQY_001379"
     CDS             complement(11158..13602)
                     /gene="dmsA"
                     /locus_tag="ACHOQY_001379"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9677514.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13841..15133)
                     /gene="serS"
                     /locus_tag="ACHOQY_001380"
     CDS             complement(13841..15133)
                     /gene="serS"
                     /locus_tag="ACHOQY_001380"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9677515.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15224..16567)
                     /gene="rarA"
                     /locus_tag="ACHOQY_001381"
                     /gene_synonym="mgsA"
     CDS             complement(15224..16567)
                     /gene="rarA"
                     /locus_tag="ACHOQY_001381"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9677516.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16578..17189)
                     /gene="lolA"
                     /locus_tag="ACHOQY_001382"
     CDS             complement(16578..17189)
                     /gene="lolA"
                     /locus_tag="ACHOQY_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9677517.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17344..21450)
                     /gene="ftsK"
                     /locus_tag="ACHOQY_001383"
     CDS             complement(17344..21450)
                     /gene="ftsK"
                     /locus_tag="ACHOQY_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9677518.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21585..22079)
                     /gene="lrp"
                     /locus_tag="ACHOQY_001384"
     CDS             complement(21585..22079)
                     /gene="lrp"
                     /locus_tag="ACHOQY_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9677519.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22623..23588
                     /gene="trxB"
                     /locus_tag="ACHOQY_001385"
     CDS             22623..23588
                     /gene="trxB"
                     /locus_tag="ACHOQY_001385"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9677520.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23711..25477
                     /gene="cydD"
                     /locus_tag="ACHOQY_001386"
     CDS             23711..25477
                     /gene="cydD"
                     /locus_tag="ACHOQY_001386"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9677521.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25478..27199
                     /gene="cydC"
                     /locus_tag="ACHOQY_001387"
     CDS             25478..27199
                     /gene="cydC"
                     /locus_tag="ACHOQY_001387"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9677522.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27241..27945
                     /gene="aat"
                     /locus_tag="ACHOQY_001388"
     CDS             27241..27945
                     /gene="aat"
                     /locus_tag="ACHOQY_001388"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9677523.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28230..28448
                     /gene="infA"
                     /locus_tag="ACHOQY_001389"
     CDS             28230..28448
                     /gene="infA"
                     /locus_tag="ACHOQY_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9677524.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            28702..28789
                     /locus_tag="ACHOQY_001390"
     tRNA            28702..28789
                     /locus_tag="ACHOQY_001390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28736..28738,aa:Ser,seq:gga)
     gene            complement(29132..31408)
                     /gene="clpA"
                     /locus_tag="ACHOQY_001391"
     CDS             complement(29132..31408)
                     /gene="clpA"
                     /locus_tag="ACHOQY_001391"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9677525.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(31439..31759)
                     /gene="clpS"
                     /locus_tag="ACHOQY_001392"
     CDS             complement(31439..31759)
                     /gene="clpS"
                     /locus_tag="ACHOQY_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9677526.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(31858..31932)
                     /gene="yljB"
                     /locus_tag="ACHOQY_001393"
     CDS             complement(31858..31932)
                     /gene="yljB"
                     /locus_tag="ACHOQY_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9677527.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            32082..32306
                     /gene="cspD"
                     /locus_tag="ACHOQY_001394"
     CDS             32082..32306
                     /gene="cspD"
                     /locus_tag="ACHOQY_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9677528.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(32379..34325)
                     /gene="macB"
                     /locus_tag="ACHOQY_001395"
     CDS             complement(32379..34325)
                     /gene="macB"
                     /locus_tag="ACHOQY_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9677529.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(34322..35437)
                     /gene="macA"
                     /locus_tag="ACHOQY_001396"
     CDS             complement(34322..35437)
                     /gene="macA"
                     /locus_tag="ACHOQY_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9677530.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            35588..36544
                     /gene="ybjX"
                     /locus_tag="ACHOQY_001397"
     CDS             35588..36544
                     /gene="ybjX"
                     /locus_tag="ACHOQY_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9677531.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(36541..38199)
                     /gene="ybjD"
                     /locus_tag="ACHOQY_001398"
     CDS             complement(36541..38199)
                     /gene="ybjD"
                     /locus_tag="ACHOQY_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9677532.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
BASE COUNT         9372 a        10202 c         9349 g         9478 t
ORIGIN      
        1 cctataagcc aggcgagata tgatctatat caatttctca tctataatgc tttgttagta
       61 tctcgtcgcc gacttaataa agagagagtt agtgtgaaag ctgacaaccc ttttgatctt
      121 ttacttcctg ctgcaatggc caaagtggcc gaagaggcgg gtgtctataa agcaacgaaa
      181 catccgctta agactttcta tctggcgatt accgccggtg ttttcatctc aatcgcattc
      241 gtcttctata tcacagcaac cactggcaca ggcacaatgc ccttcggcat ggcaaaactg
      301 gttggcggca tttgcttctc tctggggctg attctttgtg ttgtctgcgg agccgatctc
      361 tttacttcca cagtgttgat tgttgttgct aaggcgagtg ggcgcatcac ctggggtcag
      421 ttggcgaaaa actggctaaa tgtctatttt ggcaacctgg tcggcgcact gctgtttgta
      481 cttttaatgt ggctttccgg cgagtatatg accgcaaatg gtcaatgggg actaaacgtc
      541 ctacaaaccg ccgaccacaa agtgcaccat acttttattg aggccgtctg ccttggtatc
      601 ctggcaaacc tgatggtatg tctggcagta tggatgagtt attctggccg tagcctgatg
      661 gacaaagcgt tcattatggt gctgccggtc gcgatgtttg ttgccagcgg ttttgagcac
      721 agtatcgcaa acatgtttat gatcccgatg ggtattgtaa tccgcgactt cgcatctccg
      781 gaattctgga ccgctgtcgg ttctgcaccg gaaaattttt ctcacctgac cgtgatgaac
      841 ttcatcactg ataacctgat tccggttacg atcggtaata ttatcggcgg tggtttgttg
      901 gttgggttga catactgggt catttacctg cgtgaaaacg atcaccatta atggttgtcg
      961 aagtacgcag taaataaaaa atccacttaa gaaggtaggt gttacatgtc cgagcttaat
     1021 gaaaagttag ccacagcctg ggaaggtttt accaaaggtg actggcagaa tgaagtaaac
     1081 gtccgtgact tcattcagaa aaactacact ccgtacgagg gtgacgagtc cttcctggct
     1141 ggcgctactg aagcgaccac caccctgtgg gacaaagtaa tggaaggcgt taaactggaa
     1201 aaccgcactc acgcgccagt tgactttgac accgctgttg cttccaccat cacctctcac
     1261 gacgctggct acatcaacaa gcagcttgag aaaatcgttg gtctgcagac tgaagctccg
     1321 ctgaaacgtg ctcttatccc gttcggtggt atcaaaatga tcgaaggttc ctgcaaagcg
     1381 tacaaccgcg aactggatcc gatgatcaaa aaaatcttca ctgaataccg taaaactcac
     1441 aaccagggcg tgttcgacgt ttacactccg gacatcctgc gttgccgtaa atctggtgtt
     1501 ctgaccggtc tgccagatgc atatggccgt ggccgtatca tcggtgacta ccgtcgcgtt
     1561 gcgctgtacg gtatcgacta cctgatgaaa gacaaactgg cacagttcac ttctctgcag
     1621 gctgatctgg aaaacggcgt aaacctggaa cagactatcc gtctgcgcga agaaatcgct
     1681 gaacagcacc gcgctctggg tcagatgaaa gaaatggctg cgaaatacgg ctacgacatc
     1741 tctggtccgg ctaccaacgc tcaggaagct atccagtgga cttacttcgg ctacctggct
     1801 gctgttaagt ctcagaacgg tgctgcaatg tccttcggtc gtacctccac cttcctggat
     1861 gtgtacatcg aacgtgacct gaaagctggc aagatcaccg aacaagaagc gcaggaaatg
     1921 gttgaccacc tggtcatgaa actgcgtatg gttcgcttcc tgcgtactcc ggaatacgat
     1981 gaactgttct ctggcgaccc gatctgggca accgaatcta tcggtggtat gggcctcgac
     2041 ggtcgtaccc tggttaccaa aaacagcttc cgtttcctga acaccctgta caccatgggt
     2101 ccgtctccgg aaccgaacat gaccattctg tggtctgaaa aactgccgct gaacttcaag
     2161 aaattcgccg ctaaagtgtc catcgacacc tcttctctgc agtatgagaa cgatgacctg
     2221 atgcgtccgg acttcaacaa cgatgactac gctattgctt gctgcgtaag cccgatgatc
     2281 gttggtaaac aaatgcagtt cttcggtgcg cgtgcaaacc tggcgaaaac catgctgtac
     2341 gcaatcaacg gcggcgttga cgaaaaactg aaaatgcagg ttggtccgaa gtctgaaccg
     2401 atcaaaggcg atgtcctgaa ctatgatgaa gtgatggagc gcatggatca cttcatggac
     2461 tggctggcta aacagtacat cactgcactg aacatcatcc actacatgca cgacaagtac
     2521 agctacgaag cctctctgat ggcgctgcac gaccgtgacg ttatccgcac catggcgtgt
     2581 ggtatcgctg gtctgtccgt tgctgctgac tccctgtctg caatcaaata tgcgaaagtt
     2641 aaaccgattc gtgacgaaga cggtctggct atcgacttcg aaatcgaagg cgaatacccg
     2701 cagtttggta acaatgatcc gcgtgtagat gacctggctg ttgacctggt agaacgtttc
     2761 atgaagaaaa ttcagaaact gcacacctac cgtgacgcta tcccgactca gtctgttctg
     2821 accatcactt ctaacgttgt gtatggtaag aaaactggta acaccccaga cggtcgtcgt
     2881 gctggcgcgc cgttcggacc gggtgctaac ccgatgcacg gtcgtgacca gaaaggtgct
     2941 gtagcgtctc tgacttccgt tgctaaactg ccgtttgctt acgctaaaga tggtatctcc
     3001 tacaccttct ctatcgttcc gaacgcactg ggtaaagacg acgaagttcg taagaccaac
     3061 ctggctggtc tgatggatgg ttacttccac cacgaagcat ccatcgaagg tggtcagcac
     3121 ctgaacgtta acgtgatgaa ccgtgaaatg ctgctcgacg cgatggaaaa cccggaaaaa
     3181 tatccgcagc tgaccatccg tgtatctggc tacgcagtac gtttcaactc gctgactaaa
     3241 gaacagcagc aggacgttat tactcgtacc ttcactcaat ctatgtaatt agatttgact
     3301 gaaatcgtac agtaaaaagc atacaataaa ggctccacga aagtggggcc ttttttagca
     3361 cgagagcctt ttttgtcagc tatctatact ttaaggtgac tgccaaaaca gactcgacgt
     3421 agccttcgag ctgcgcacca acacggcctc agatgggcca catctggaga aacaccgcaa
     3481 tgtcagttat tggtcgcatt cactcctttg aatcctgtgg aaccgtagac ggcccaggta
     3541 ttcgctttat cacctttttc cagggctgcc tgatgcgctg cctgtattgt cataaccgcg
     3601 acacctggga tacgcatggc ggtaaagaag ttaccgttga agatttaatg aaagaggtgg
     3661 tgacctatcg ccactttatg aacgcttccg gcggcggcgt taccgcatcc ggcggtgagg
     3721 caatcctaca agctgagttt gttcgtgact ggttccgcgc ctgcaaaaaa gaaggcattc
     3781 atacctgtct ggacaccaac ggttttgttc gtcgttacga tccggtgatt gatgaactgc
     3841 tggaagtaac cgacctggta atgctcgatc tcaaacagat gaacgacgag atccaccaaa
     3901 atctggttgg agtttccaac caccgcacgc tggagttcgc caaatatctg gcgaacaaaa
     3961 atgtgaaggt gtggatccgc tacgttgttg tcccaggctg gtctgacgat gacgattcag
     4021 cgcatcgcct cggtgaattt acccgtgata tgggcaacgt tgagaaaatc gaactcctcc
     4081 cctaccacga gctgggcaaa cacaaatggg tggcaatggg tgaagagtac aaactcgacg
     4141 gtgttaaacc acctaagaaa gagaccatgg aacgcgtgaa aggcattctt gagcagtacg
     4201 gtcataaggt aatgttctaa tttccccgta aagcggcaac tttattgagt tgccgctttt
     4261 ttattccgcg cttcattctg ctacagcgtt tttaacacat tttatagtgc atctaccata
     4321 tcccgtacct gagataataa cgactgataa tagctcgcat gaagttcttg cccatcgaat
     4381 accactgtat tacacaagaa agtgacatcg gcatcttcaa taccaagata actgcacata
     4441 tttttcaaat aatcgcttat atttttttcc cagcccatct ggacaaatga ttctttgtct
     4501 cctccaacca gcgccaccca acgaacttta ttgaatggta atttgtgccc atctccataa
     4561 gccagcccat tattccatac tctgtcaata tatcctttta gcattgccgg gaagctatac
     4621 caccagagag gaaaaaccac cactaacgtg tcatgttcaa gcagctctga ataaagctga
     4681 tgaacctctg gagaataacg tttatccata ttcttccagt ctggttcatc ttccggcgtc
     4741 atcactgggt tgaaattacg ccgatataaa tcgagttccg tcacctgtat tttccgctcc
     4801 attgcccgct gatggatcgc atcagcaata tttgcggtca atgaatcatg acgaggatgg
     4861 gcccatacca aataaatacg ttcagactgc ataattttac ctccacgtta agttctggag
     4921 cgcactttac gcaggcggca taatcctgta aacgtgatga ttcaggatga gttaatgaag
     4981 gattttcact aatgcggatg aatatgtctg actttgccac tttctttgcc gtcgcccgta
     5041 atcaaagctt tcgtgcagcg ggcgatgagt taggcttatc ctcgtccgcc attagccata
     5101 gtattaaaac actggaacaa cgtcttaaaa ttcgcttatt caatcggaca acccgaagcg
     5161 tatcgttaac ggaagcgggt tctaatctct atgaacgatt gcgcccagct ttcgacgaaa
     5221 ttcagatcat gttagatgag atgaatgatt ttcgtctgac accgacagga acgctcaaaa
     5281 tcaatgccgc ccgggtagct gcacgtatat ttttaatgcc tttactggtt ggtttcacac
     5341 gtgaatatcc tgatattaaa gtcgagctca caactgacga ttctcttgtc gatatcgtcc
     5401 aacagggctt tgacgctggc gttcgactta gtggcatcgt cgaaaaagat atgatcagcg
     5461 tggcgatcgg cccaccagtg aaattatgtg tagcagcaac gccagagtac tttgcaagat
     5521 atggcaaacc ccgccatcct catgatctgc ttaatcatca atgtgttgtt tttcgttatc
     5581 ccagcggcaa acctttccac tggcaatttg caaaggagct ggaaattgct gtcgcaggaa
     5641 atatcattct ggatgatgtt gatgctgaac tggaggccgt tttgatgggt gctggtatcg
     5701 ggtatctgct ctatgaacag atcaaggagt accttgatac tggtcgttta gaatgtgtac
     5761 tgggagactg gagcacagag cgtccaggct tccagatcta ttatccaaac cgccagtata
     5821 tgtcctgcgg cttgcgcgca tttctcgatt atgtaaaaac gggtcagatc tgccagagtc
     5881 agcgtcaccg accacaataa ttatttacta ttcgctttac gcgccagcag cgggaaaatc
     5941 agtcctaatc ctaccagcac aaacggcgtt gcaacattca gcgccaactg gaaggtccac
     6001 tctgcagtga aggcttccat tttcgggaag atccccgtca aacaggcaaa ggcggtaaag
     6061 gcaaaacacc aaatcccgac ggtcattgcc agaggcttat tacgaataaa gacatattct
     6121 ggtttatatt tctgcgccag gcgaacgact gcaataaatg caacaaatac ccataaataa
     6181 cgcagcggca tcactaccga gttaagattc aacagccatt tatagagatt gttcatatca
     6241 ccgatgccga gagtcggcag cataatcaga attgccacca gcaccagggt cagaaaatag
     6301 ccattaacgg gcgtaccaga agcgttggta cgacataaac tggctggaat atatttgctg
     6361 tcagcatcac ctaatagcac tttaagcggg gcatcaatcg agaataccag cgcagctact
     6421 tgtcccaggg tattcgcaat ggcgtaaatc accattaaag tattacccat gttgtaatac
     6481 tcgcccagct tctgaaaggc gtaatattga ccgttagtca ttaagtcatc cgggatatta
     6541 cgcgagtcaa acatcatccc catcgccagt gagcccagaa tggcacaaac cgcaaccatc
     6601 accgccaggc atagcatccc ttttggaaat tcttttcctg ggttgcgcgt ttgattaacg
     6661 taaggagaaa tcttctctgc tccgccaacc gcgaaaacca gcattgaaat agtggtaatg
     6721 taggtaaagt cgatatgagg aatgaacgtt tcccaggtaa tgtttgtggt cgcaatatgc
     6781 acttcagtaa tcgcagacgc ggttaccgcc atcgccacat acaggagcga cataacaaac
     6841 atcgcaatcc ctgccacaga accgacgatt ttcagcgatt tcataccgcg tgaagcaacc
     6901 cacataaaga agataaacag caccagcgtt aacccctgta acgctacgac tgaatattct
     6961 ttgattagcg aaccgtcgcc tttcatcgcc caaccgagcg caatcagaat tgcctggggt
     7021 ttttgtgcca gaaagggaat atgcaccacc cagtaggtcc acgcggcgag ataagccagt
     7081 ccgggtccca tcgtgtgttt aatccaggta ctgaccccgc ccttcccatc tttgaaggtc
     7141 gagcctaact gaccaacaat tagcgcataa ggtgtgaaat agagtgcaaa gataaacacc
     7201 catgaaaaaa caaccaccag cccctggttg gcatagttat taacaacgtt gccaaaaccc
     7261 cagacagtga taaaagacat cagcgcaatg ttgtaccatc gcaactgttt ttcctgaaca
     7321 ttcccagcca tagacaaatc cttcgccaaa attaaagggg catctgtaaa acgagggatt
     7381 atgcgcggca aataagaagt gcggaaatag cgaaatgcaa aattataacc tgctgcacat
     7441 tttattttta aacgaataaa atttggaata aagcaagtca gatagaagaa aaaaaaagca
     7501 gagtgtgaaa cagtaagttc acactctgct tgaattcatt tacacgtgag caacgggttt
     7561 cggcgtatga ccggcgttgc gcagcagcat cagcaaatag ataaacgata cgctggcgat
     7621 catgataaac aataaattat cggagaaatt ctgcattagc atagcggtaa atgacgggcc
     7681 aagcagactt cccacagtat agctcaacag taaggcctgg ttcatcgcca ccagttgatg
     7741 atgttcaact ttctcgcaag cccatgccat cgccaccgga tatagcgtaa agccagcggc
     7801 accgaggatg aataacgctg gggccatcgc cgcctggcta agcatcgcga tactgccgag
     7861 aatgacgaca aagacctgaa cacgcaacac cagcagtcga ccaaacttat ccgccagacg
     7921 tccaatcggc cattgtccaa ggatacccgc actgaccagt accgccatcc agaaaccaat
     7981 gctggcattg ctcacccctt tgtgattgag gtacagcggc atcaggccat atagagatcc
     8041 cagaacgatt cctgagataa tgcagccatt cacgccaagc cgcgcctgac ggagttttag
     8101 cattgacgta attgacgtcg aatcatggtt ttcagcctgc tgattcagca cacgcgtaaa
     8161 caacagcggt aagatccctg ccaacgtcaa acctgtaacc cacggcaata cggacatcag
     8221 ctcggttgaa actttgctga ccagtaactg gcctaaaaac gttcccacgt aataaaccat
     8281 catatacgca gcaagcaaac gcccacggtt acgtgacgtc ccactgcaca tcagcgcgct
     8341 ctcaacaacc acccaaatca tggcacagcc gacgcccgcg acaaaacgcc aagccaacca
     8401 gctccagaat ccaatcatca ggccaaggcc ggcacagcca gcggcaaaaa tgaaggaggc
     8461 cagataatag ctgcggttaa agccaatgcg cttaatgaca taccctgtca gcaatgtacc
     8521 gacaaggttg ccggtaaaat aggatgagct gacaacgcct acctgccatg tggacatgtg
     8581 ttcctgggcg agccaaagcg gcacgagtgt atttaacacc gcgatcgcca gagtcaacaa
     8641 aagcaggcca gacagcaaaa gcatgacagg ccgggtatac gtggacatga attaaaaccg
     8701 tgaggaagtt caaatttcat gcgcatcatg ccaccgccaa aaacattgtc aatgggtgaa
     8761 aatacgcgca tatcgcagcg gcggtattat cgatttatat tactcattca taacgctaat
     8821 gctgaagaga tgattaatct catctgattg tttttattga tctaacacaa atttcatacg
     8881 cttcgttcag tcgaaaaatt tcatggtcac agcaaactca tatttcccgt ctatgctttc
     8941 agagcaatgc atcacgtctg accgatttca gacgtaccac tgactctcga aaggagaaga
     9001 ggatgaccaa accgtatgtt cgtcttgata aaaatgatgc tgccgttttg cttgttgatc
     9061 atcaggctgg tttactttcc cttgtacggg atatcgaacc cgataagttt aaaaataacg
     9121 tgctggcgct gggtgacctg gctaagtatt tcaacttacc tactattctt accaccagtt
     9181 ttgaaaccgg tcctaacggt ccattagttc cagaactgaa agcacaattc cccgatgcac
     9241 cttacattgc tcgcccggga aatattaacg cctgggataa cgaagatttt gtaaaagcag
     9301 tcaaagcgac aggtaaaaaa cagttaatta ttgccggtgt ggtaaccgaa gtttgcgtgg
     9361 cattcccggc gctttcagcc attgaagaag ggtttgatgt ctttgttgtg actgacgcgt
     9421 cagggacctt taatgaaatt acccggcatt cggcatggga tcgcatgtcg caagcaggtg
     9481 cgcaattgat gacatggttc ggcgtggcgt gcgagctgca ccgtgactgg cgtaatgata
     9541 ttgaaggact ggcgacgttg ttctctaacc atattcctga ttatcgtaat ttgatgacca
     9601 gttatgacac gttaacgaag cagaaataaa agagaaccgg ggccgaaacc ccggttgtta
     9661 ctgattagct tgcgacggcc atccccacgg tcatatgcaa accatagaat acgccgcgac
     9721 cgattaattc ccctgccaga agcagaatga atgagacgga tagtagcgga accgcaggct
     9781 gataaccttt tagctgtggt gcaatccaca agcacagggc aacggccaaa agcacgatcc
     9841 gccaggacat cagcgcacca tagtccggca ccagcgctgc ggcctgctgc acagaactat
     9901 gaatagttgc cagctctgtg ccctgcatca ctgacatcac gccactcacc accagtgcca
     9961 gtacagaaat ggctggcagc agacgcatcg cccagccatc gacgccagcc agactcaaca
    10021 acaggtaacc cagtaacggg ccgcccataa acatcgtcag gaagaagccc atcggcgtcc
    10081 agatgctgta ccaggtcgga acggtatcaa tgctgttata cacacgcacc atcatccaga
    10141 caaagatgac gccaagaacc atcgtcacta tcagccacag cgtacgcaat gccggtgaca
    10201 gctttttcag cattgccagc aaccagccga tgccgcctac ggcaaaaaag atcgaaccgc
    10261 tggcgatttc gttactgagt gctgaagccc ctacccggtt gagcgagtta aaagcgcgca
    10321 ttggtgaacc aagatggagc atagaggcga taaagccaat gcccatcagc acccataaac
    10381 caaacatgca ggcgataaca cgctgctggg cttctgctcg caggtcgcct ttgagcagcg
    10441 ccaaagccag aacgataaaa ccacctgcta cacattgccc gaagaccgtg aagatcatca
    10501 gcggccattc atgccatcca cttcccatct cacacctcct tcgggtttgc cagatagcca
    10561 gtggtatccc cggtcgggcg gctattggca ttgggtttga tcacaatatt cggtttggta
    10621 aagtgagctc gcggcaacgg cgcaacggcc gccagatcgc catgtttttt acgcagctcg
    10681 tcgatagggc cgaaatccag cgcccgcagc ggacaagatt caacacagat cggcttttta
    10741 ccctccgcaa cacggtcata acaaccatcg catttggtca tatggccttt cgtttcgtta
    10801 tattgcggtg cgccatacgg gcaagccata tggcagtagc ggcagccaat gcacacatct
    10861 tcatcgacca caacaaaacc atcttcacgt ttatgcatcg caccgctcgg gcagacttta
    10921 gtacaagccg gatcttcgca gtggttacat gaaatcgaca gatagtaggc aaacacgttc
    10981 tggtgccaga caccgttatc ttcctgccag tcgccgccag catattcata aatgcggcgg
    11041 aagctgactt ctggcgtcaa atctttgtag tctttacagg ccagctcgca ggttttgcaa
    11101 ccggtgcaac ggctggaatc aataaaaaat ccatactggg ttgtcatcgg ttactcctta
    11161 caccttttca acctgaacaa ggtttgtatg tgacggattc cccttagcga gaggagacgg
    11221 acgttgagtg gtcagtacgt tgatacaacc gcccttatcg acacgttttg tatccgggtc
    11281 ataccaggca ccttcaccca gtgcgaccac acccggcatc attcgtggcg tcactttcgc
    11341 ctcaatatga acctcaccac gatcgttaaa gatcctgact ttatcgccgt tgttgatacc
    11401 gcgtttttgc gcatcaagcg ggttgatcca catttcctga cggcaagctg ctttcagcac
    11461 atcaacgttg ccgtaagttg agtgaacgcg agatttatag tggaaacccg taagctgcag
    11521 cggatactgt ttgttcagcg gatcctgata actttcaaag cccggcgtgt agatcggcag
    11581 tggatcgatc acatcgcctt ctggcaattc ccaggtagcg gcaatgtcag ccagcgcctg
    11641 cgaataaatc tcaattttgc ccgatggcgt agtcagtggg tttgcctgcg gatcttcacg
    11701 gaaggcttta taagcaacgt gatgcccttg tgggtcgcgc tttttaaaga tcccctgctt
    11761 gcggaactct tcaaacgttg gcagttcagg aatcgcttcc cgcgactggg catacagatg
    11821 ccgcatccac tcttcctggg tacggccttc agtaaactgt tgctcaacgc caagacgttt
    11881 tgccagttcg ctggtcattt catagatggt cttacattca aagcgcggtt taatcacctg
    11941 atcgttgaaa atcacgtaag acatattccc gcaggatgca tccagtgcaa agtccatctg
    12001 ttcggaagcg gtgcagtcag gcagcaggat gtcagcatat ttcgccgatg aggtcatgtg
    12061 gcagtcgata accacaatca gctcgcactt cttatcatcc tgaaggattt catgggtacg
    12121 gttgatttca gaatgctggt taatcaggca gttaccggca tagttccaga tcattttgat
    12181 cggcacatcc agcttatctt tcccgcgtac accatcacgc agcgccgtca tttccgggcc
    12241 acgttcaatg gcatcggtcc acataaacat cgaaatgctg gtctggatcg ggttttccaa
    12301 ggtcggcata cggacaaacg gtaagctgta tgaaccttca cgcgcgccgc tgttgcctcc
    12361 gttaatacca acgttaccgg tcagaatcgc cagcatcgag atagcacggg ttgcgatttc
    12421 accgttagcg tgacgctgcg ggccccatcc ctggctgata aacgccggtt tggtactgcc
    12481 gatttcacga gccagtttga tgattttgtc tgccggaaca ccggtgattt gtgaggccca
    12541 ttccggtgtt ttagccacgc catctggccc ttcacccaga atataagctt tatagtggcc
    12601 atttttcggc gcactggctg gcagggtttt ctcatcgtag ccaacgcaat atttatcgag
    12661 gaatgcctga tccaccaggt tttcagtgat catgacgtac gccagaccgt taaccagtgc
    12721 ggcatctgtt cccggacgaa tagggatcca ctcatcttcg cgcccggcac cggtgtcggt
    12781 atagcgcgga tcaatgatga tcatgcgggc attagatttc tggcgtgcct gttcaagata
    12841 gtaagtcacc ccaccgccac tcattcgcgt ttcgccaggg ttattaccaa acagcactac
    12901 cagcttactg ttttcgatat ccgacgggct gttgccatct gcccagccgc cgtaggtata
    12961 gttcaaacct tccgcaattt gcgcggagga gtagtcgccg taatggttca gatagccgcc
    13021 gcagcagttc atcagccgcg cgaccagggt atttcccggc ggccaggagc gggtcatggt
    13081 gccgcccagc gtaccggtgc catagttcag atagatagac tcgttgccgt actctttgat
    13141 caggcgctgc atattggtcg cgatgatgtc gtaggcttct tcccagctaa tgcgctcgaa
    13201 tttgccttca ccgcgcgccc cgactcgttt catcggatat ttcaggcggt ccggattgta
    13261 gacacggcga cgcatggaac gcccacgcag gcaggcgcga acctggtgca ggccgtcgta
    13321 attgtcatcg ccggtattgt ccgtttcgac atatttgatt tcaccgtcca cgacgtgcat
    13381 acgtagcggg cagcgactac cacagttaac tgtacaggcg ctccagataa ccttttcgtc
    13441 tgattttgtt ggaatggcgc tatcgacagc gtgcgcaatc cgactaaaag gtaatgttaa
    13501 tgcgctgctg gccattgcca ggccgccgat cgctgtcgtt tttaccaaac cacggcgact
    13561 cacctcagca gccaataccg catcagggat tttcgttttc ataatggctc actcaagctt
    13621 gctcacgatt gattccagta acaaaccgta ttgtgtaatt atttctatag cagctcgcta
    13681 tataggcttg tatacatcga ataatttgaa aatcaaagag ataggtagat tttcactcga
    13741 aagtagtatt acgtgtaagt gaggagtaat tattgttcgg tatcaaaggg agacaaaaaa
    13801 agcgcccgca ggcgcttttt agattcagaa aaattgggta ttagccaata tattccagtc
    13861 cgttcatata cggacgcaga acttctggta cttcaatacg accatcagcc tgctgatagt
    13921 tttccattac tgcaaccagc gtacgaccaa cagccagtcc agaaccgttc agggtatgaa
    13981 ccagacgggt tttcttgtcc gacttgctgc ggcagcgtgc ctgcatacga cgcgcctgga
    14041 aatcccaaac gttggagcag gaagagatct cacggtaggt gttctgtgcc gggatccata
    14101 cttccaggtc gtaagttttg caagcgccaa agcccatgtc gccagtgcaa aggatgattt
    14161 tacggtacgg caggcccaac aactgcagga ctttttctgc atgaccagtc atctcttcca
    14221 gcgccgccat tgagtcttcc gggcgcacga tctgcaccat ttcaactttg tcgaactggt
    14281 gcatacggat cagaccacgg gtgtcacgac catatgaacc ggcttcagaa cggaagcatg
    14341 gggtgtgggc ggtcatctta attggcagat catcttcatc gatgatttca ccgcgcacca
    14401 ggttagtcag cggaacttct gccgttggga tcagcgcata gttactggtg tctgcttctt
    14461 cttccagcgg acgagtatga aacagatcgc cagcaaattt cggcagctga cccgtaccgt
    14521 acagcgtgtc ctggttaacc aggtacggaa catagttctc actgtagcca tgctgttcgg
    14581 tatgcagatc cagcataaac tgcgacagtg cgcggtgcat gcgagcaatc tgccctttca
    14641 ttaccacaaa gcgggaacca gtcagcttaa ctgcagctgc aaagtcgagg ccagagtgca
    14701 tttcacccag cgtcacatgg tcacgaactt caaagtcaaa ctcacgcggg gtgccccagc
    14761 ggctgacttc gacgttgtca ttttcgtctt tacctaccgg cacttcatct gcaggcaggt
    14821 tagggatggt cagcgcgata tcgcgaattt cagcctgtaa agcatccagc tcggcttttg
    14881 ctgcatccag ctcttcgccc agtttgttca cttccagacg taaaggctcg atatcttccc
    14941 cgcgcgcttt cgcctggcca atggatttcg atcgggagtt acgctccgct tgcaggtttt
    15001 ccgttttgac ctgcaatact ttacgacgct cttcaagagc gcccagctta tctacatcca
    15061 gcttaaagcc ccggcgtgcc agtttttcag cgactgcgtc tggctcatta cgcagcagat
    15121 tgggatcgag catgcttatc ctgtgcttat cgaattaaat gggagatgtg gccacagcct
    15181 gcgaccacag ggatacagta acaacattac cgcaacgata acattaacgg tagcgtttta
    15241 tggggctatt ttgatcctgt tcagccagcc aggcgagctt ttcgccaatc ttgccttcaa
    15301 ggcccctgtt tgtcgggaaa taatagcgtg tttgtgctat ttccggcggg aagtaaacct
    15361 caccggcagc ataagcgttt gcttcatcat gagcgtaacg atattcctgc ccgtagccca
    15421 tttccttcat taatttcgtc ggcgcattac gcaaatgaac cggcacgtca taatccggac
    15481 gttcgcgagc atccgccagc gcagctttaa acgcagtgta gacagcgttg ctttttggcg
    15541 cgcaggccag gtaaacaatc gcctgagcaa tggcgcgttc accttccgcc gggccaacgc
    15601 gagtaaagca atcccaggcc gcaattgcca cctgcatcgc ccgtgggtcg gcattgccga
    15661 catcttcgga agcaattgcc aggcaacgac gcgcgacata taacggatcg ccaccagcgg
    15721 taataattcg cgcataccag tagagcgccg catcgggtgc gctaccacgt accgacttat
    15781 gcagtgcgga aatgagatcg taaaagcgat cgcctttgtt atcaaagcgg gcgcttcgct
    15841 caccggcgat ttcggtcaat aattcaggct tcaggacccg cttaccgcta tcattgactt
    15901 cggccatatc cgccatcatt tccagcgtat ttaacgcccg gcgcgcgtcg ccattcacca
    15961 gttcagcaat ggcgcgtcgt gtttcatctg gcagaacaat atcctgacca ccataaccac
    16021 gggtcttgtc ttccatcgcc tgagttagta cttgctcaat atcctctgta cccagggatt
    16081 tcaacagata gacacgggca cgggaaagca gtgccgaatt aagctcaaac gacgggtttt
    16141 cagtggttgc gccaataaaa gtgatggtgc cgtcttcaat atgtggcaga aatgcatcct
    16201 gctggctttt gttgaatcgg tgcacttcgt caacaaaaag aatagtgcgg cgacctgcat
    16261 tgcggttttg ccgggcgcgc tcgatcgcct cgcgaatctc tttcacgcca gaggtgacgg
    16321 cagaaatacg ttccacatca gcgttcgcat agcgggcaat cacttcagcg agggttgttt
    16381 tgccggtacc cggtggcccc cagaggatca tagaatgtaa atgcccggct tcgatagcgc
    16441 gcggcaacgg cttccccgca gccagcaaat gttgctggcc gatatactgt gctaaatttt
    16501 ctggccgcat acgcgcggcc agaggttgaa aagtattatc cgaaaaatcg agcgacagat
    16561 tgctcactca ggtgcctcta cttacgttga tcatctaccg tgacgccttg cggcggggtg
    16621 aaggtaaatt tcgctgcatc cacagcccca ttttgctggg atttcagttg ataactgctg
    16681 cgctgatcgt cctgctccac cgcgctaaac tgatggattg tgccatcacg tcccacgtta
    16741 atggtgaact gcttcagatt gccattgctg gctttcggcg tcaggacaaa gtcatcgcca
    16801 ttctgtttga tattgtactg ctgccagtcg ctggactggt tgcgggcaat cagcataaac
    16861 ggcgtattac cggtggcatc tttcagccag gttgccgtag cttgctcaac gaacgggtta
    16921 tagaaccaca gtgttttacc gtcagaaacc agaatgcttt catcaggttg tgtcatatgc
    16981 cagttgaata agtttggacg tttcacccac agatcgccct gaccttcctg caccgccgcg
    17041 ccgctaccgt cagtcacttt ttgtgtgaag ctggcgtgga agctgctgac tttatccagg
    17101 cggcttttca gatcgcttgc ggcatcagcc caaacgctgc ttgctactaa gcttgagagt
    17161 aatgcacagg tgatggcaat ttttttcatc attattcctc aaattacgtc actcccgatc
    17221 gggagttccc tctactctct attccaggct aataatcagc ttgaggaaag aagaaaatac
    17281 tgaattttca gttgatagag tgccggatgc gacgctaccg cgccttatcc ggcaatgcat
    17341 taattagtca aacggcggtg gggccagcac ttcacgatta ccgttgtgcc cctgttcgct
    17401 gacaatcccc tgcgcttcca tctgttcgat aatacgcgct gcacggttat aaccaatgcg
    17461 gaactgacgc tgtacgccag aaattgacgc tttgcgtttt tcagtgacaa actgcaccgc
    17521 ctgatcgaac aacggatcca gttcttcagc gccatcgaaa ccgcccgcgc caccttcgct
    17581 ttcactgtcg gaagtgatgc catcaacata ctgtgggcga ccacgcgctt tccagtcctg
    17641 caccacggca tgaacttcct gatcgcgaac aaaagcacca tggacacgta ccggcaacgt
    17701 ggagttcggc ccagagtaga gcatatcccc catccccagc agtgattccg cgccagcctg
    17761 atcaagaatg gtacgtgagt caatcttact ggatacggta aaggcgatac gggtcggaat
    17821 attcgcttta atcagaccag taataacatc aaccgatgga cgctgagttg ccagtacgag
    17881 gtggataccc gcggcacggg ctttttgcgc cagacgtgct atcagctctt ccactttttt
    17941 acctaccgtc atcatcaggt cggcaaattc gtcaaccaac accacaatgt atggttcttt
    18001 tttcagcacc ggatgctggg catccatact gtcacccggc ttccagtacg ggtctggaat
    18061 cggacgcatc atgcgatcgg cttcagcaat tttttcgtta taacccgcca gattacgcac
    18121 acccagcgca gacatcagtt tataccgacg ctccatctcg ttaacacacc agcgcagcgc
    18181 gttggcggca tctttcatat cagtaacgac ttccgttaac agatgcggaa tgccttcata
    18241 aaccgaaagc tccagcattt tcgggtcgat catgatgaaa cgcacatctt ctggctgtgc
    18301 tttataaagc atgctcagga tcatcgcgtt cacaccgaca gatttaccgg aaccggtagt
    18361 ccccgcaacc aacaagtgcg gcattttcgc cagatcggca accaccggct caccggcgat
    18421 atctttaccc agcaccacgg ttaatggcga cggattatcg cggaatttgg cgttatccaa
    18481 aacttcgcgc agataaacgg tttgtcgttt tttatttggt aactccagac ctacataggg
    18541 tttgccagga ataacttcaa cgacacgcac cgccaccgtc gaaagtgaac gggcaaggtc
    18601 ccgtgacaag ttagaaatgc gcgccgcttt tacgccagga gccaggttaa gttcaaagcg
    18661 agtgataacc ggccccggag agtaattgac gacatcggct ttaatacgga aatcagccag
    18721 acgcgcttcc accaggcgag ccatttgttc aagcgcaaag gtatctaccg gctccacttc
    18781 gctcggcggc ggtgtcagca aatccagaga aggcagcggc gtcgtcggtt tatgcaacgg
    18841 acggctgtcg ccattacgca tcaacagcgg atgaagcagg gtatcctgct gctgcggtgc
    18901 aaccggttgt tgcggctgtt gatactgcgg ctgcggagca actggttgtt gcggctgctg
    18961 atattgcggc tgtggcgcaa ccggttgttg cggctgctga tattgtggct gcggcgcaac
    19021 ctgctgttgt ggctgctgat actgcggctg cggcgcaacc ggctgttgcg gctgctgata
    19081 ttgctgctgt ggcggaactg gttgttgcgg ctgctgatac tgctgctgcg gtgcaaccgg
    19141 ttgttgcggc tgctgtacag gttcaacaat tggcgtaaac aacggttcgt gtggaccatc
    19201 atccagcaac gctttcattg gcgaaaattc aaaatcatcc agcgagaacg gattcgcccc
    19261 agccggttgt tcgccggaat aacgttgttg ttgagtttgc gcaaactgac gagccagttc
    19321 agcctctgcc gcagcatctg catcttctgc gtttacgggc acatcatgtt gatactgttc
    19381 gccatagcgt tgctgctgtg tctgggcgaa ctgacgtgcc agttcatcct gctgcatcgc
    19441 atcgatttca tcatcgttat actgatcgcc agaatcatac tgattgcgct gggcttcacg
    19501 ggctttctct tccgccgcac gctgtgaggg cagcttaata ccgtaagacg ccagttcacg
    19561 acgagttggc acgcggatac gtttcggtcg tggcaactgc gggccaatcc cctctttgac
    19621 ttgcggacgc ggtccaccgc tatttgccag actgaagact ggcgcggcaa cggttgcggc
    19681 agcccccgtc gccagtgtcg cttttttcac gccagatgcc agcggagaaa cagcggcagc
    19741 ggcttctact ggaggtactg ctgcaacaga aggcgctttc aacgaagatt tgatcggttc
    19801 tggttcttta accggttctg gaatcggttg ataccaggcc gcaagttgtt cacgttcacg
    19861 ggctcgcttc tcttccactt cttcaaaata gtaaagcggc ggacgcgcgg gttttgtctc
    19921 ttctacaacg ggttcaggct ccacaacagg ctgctgttca acggattgcg gctgttggta
    19981 caacggctcc tgagcggctg gttgctgata agtttgctca gtctggtatg tagactgtgg
    20041 agcaaaagtg gattgctgct cttcggcttg ccaggcgtta cctgccaccg gttgttctgg
    20101 cgcaggggcg taatacggct gttgtgcagg ctgttccggc gcagttgcgt aatacggttg
    20161 ctgaacaggt tgttccgccg caggagcgta gtaaggttgt tgcgcaggtt gttcagctgc
    20221 aggtgcataa tacgactgct gcggctgtac tggttgttgc agcggctcat tatattgcac
    20281 tgcaggctgc gcatattgtg actgctgtgg gtaaccttcc ggtgcaggag caataaccgg
    20341 ctctcccgtt tgtggacccg gtacaggctg ccaggctact gtaggttgcg agggtggaac
    20401 atcaacagag gcaacaggcg gcgtctgagt cacaggttca accggcgcag cccagctttg
    20461 tgtcgccgtg gtagcagcag ctgctacagc gacaggttcg gtaattggcg caccgtttaa
    20521 taatggatcg tattcgtcat attctggctg cgttgcacga ttgcccgaaa ataggacgtc
    20581 gtccgggtcg gcagccacac cgcgtgcagt gtaggtaatc tcttcgtcat catccatccg
    20641 cttaccggag aacaacgcag cgtctgtttg ccgccccatc ggattaatga atttttccgc
    20701 caaccgttta cgacgcgcta gcgcgccgcg aagaatacgg gcacggcgtg attcatgctg
    20761 tttgccgtgg ttttcatctt catactcttc gtcatcttca tattcatctt catcgaccca
    20821 ggtatcatcg cgacgggtac gattactggc gaaggtgaga atgtttaaaa tccagccgcc
    20881 gagtttttca gcaatggtca cccatgacca accggtgaac agcgtcaggc ccgcagccca
    20941 aacgcagagc agcgcaatag ttcccccgct actgtgtagc agtggttgta gcgtagtact
    21001 tagtaagctg ccaatgacgc caccggaggc aaaataccag atatcgtcag cgttgattgc
    21061 cgccagacca caggaggtaa ggatgagcgc caaaacgcca atgatgcgta gcgaaacggc
    21121 aaaataatca atgtattcgt cgctggactg atgacgccag gcaaaccaac aaccgccgac
    21181 aataatgacg ggaatggtgt aagccatcac gccaaaaata aagaacagcg tatctgccaa
    21241 ccacgcaccg ggcatcccac ctaaattatg gataggttca tgccaggccg tttgcgacca
    21301 gctggggtcc gaagggttaa agcttagtaa ggcagccatc aaccagacgg caaacaggac
    21361 aataaggatc agcaacgcct ccagaaggcg gcggccgcta cttaactttg tcaatgtgac
    21421 ttctttgtct tcagtgtatt cctggctcaa gaaaggctct ccaggttaca ggcttttcct
    21481 gcccgatgct aaaacggaca acagtaccag gttaccccag tactgttgct gtatggatta
    21541 acaggagtgt aatcaaaata cgccgatttt gcacctgttc cgtgttagcg cgtcttaata
    21601 accagacgat tactctgctt gacttcttcc ataacaacgt atgtccgtgt gtcattgacg
    21661 ccaggcagac gcagcagggt ttcccccagc aacttacggt aagctgacat atccggcacg
    21721 cgtgttttca acaggtagtc gaaatcacca gatactaaat gacactcctg aatttcttca
    21781 agtttttgta cagcggtatt gaattgttca aacacatccg gtgcgccacg attcagagta
    21841 atctcaacga ataccagaag tgatgcatcc agataatggg ggttcagcag cgccgtatag
    21901 ccctgaataa acccttgtct ttccagccga cgcacacgct caaggcacgg cgttggggaa
    21961 agtcccacac gtttagaaag ctcgacgtta gaaatacgcc catccttttg caactcatta
    22021 agaatgttac gatcgatacg gtcgagatct ttgccagggc gcttcttgct atctaccatt
    22081 attattgtct ctctgtattc cttccctact cctgtctgac cctgaaacat cactgttcag
    22141 agtcgctacc cgtcacgata gaccgaaatc atgaagattc acggcgcgca atgcctgggt
    22201 ctacggtgag aagaccgttg ccttgtggca gctatcgaca tcacgaatgg cgtctgtcca
    22261 cgccatacgt agatttcact agcacggtaa acatggtatt tacatgcatg gttatgcgca
    22321 tataagaata ctgttttttt cttcctcgaa tgttttcgca aaacaccagg ggattgtcaa
    22381 agcaaaacat cgatttttag tacaacatgc cagatattca ttaacagggg cgtttattca
    22441 tcatttaatc ggcttataaa gcgggaaatt taagtagcag aggtgataat gacgggagaa
    22501 tttgacctgc ttatcgatgc tgccgattgc acaaattgtt aacaaaatcg tcatactctt
    22561 tttttacgtc tgtaaattcc ctacaatcct gcccattgtc tgccaacaac tatggggatc
    22621 tcatgggcac gaccaaacac agtaaactgc ttatcctggg ttcaggcccg gcgggataca
    22681 ccgctgctgt ctacgcggcg cgcgccaacc tgcaacctgt gctgattacc ggcatggaaa
    22741 aaggcggcca actgaccacc accacggaag tggaaaactg gcctggcgat ccaaacgatc
    22801 tgaccggtcc gttattaatg gagcgcatgc acgaacatgc caccaagttt gaaactgaga
    22861 tcatttttga tcatatcaac aaggtggatc tgcaaaaccg tccgttccgt ctgaatggcg
    22921 ataacggcga atacacttgc gacgcgctga ttattgccac cggagcttct gcacgctatc
    22981 tcggcctgcc ctctgaagaa gcctttaaag gcagtggggt ttctgcttgt gcaacctgcg
    23041 acggtttctt ctatcgcaac cagaaagttg cggtcatcgg cggcggcaat accgcggttg
    23101 aagaggcgct gtatctgtct aacatcgctt cggaagtgca tcttattcac cgccgtgacg
    23161 gtttccgcgc ggaaaaaatc cttattaagc gtctgatgga taaagtggag aacggcaaca
    23221 tcattctgca caccaaccgt acgctggaag aagtgaccgg cgatcaaatg ggtgtcactg
    23281 gcgttcgtct gcgcgatacg caaaacagcg ataacatcga gtcactcgac gttgctggtc
    23341 tgtttgttgc tatcggtcac agcccgaata ctgccatttt cgaagggcag ctggaactgg
    23401 aaaacggcta catcaaagtg cagtcgggta ttcatggtaa tgccacccag accagcattc
    23461 ctggcgtctt tgccgcgggt gacgtgatgg atcatattta tcgtcaggcc attacttcgg
    23521 ccggtacagg ctgcatggca gcacttgatg cggaacgcta cctcgatggt ttagctgacg
    23581 caaaataatt ttacaaatca gtaacaaaag taaagaaggc gacaccatgc gactatgggt
    23641 cgcctttatt ttttccccgt tgtaacattg ctctgcaaat aattctgata actcacctgc
    23701 taagcgtgca atgaataaat cccgtcaaaa agagttaacc cgctggttaa aacagcaaag
    23761 cgtcatctcc caacgttggc tgaatatttc tcgtctgctg ggctttgtaa gcggcatatt
    23821 gatcattgcc caggcctggt tcatggcgcg aattctgcaa catatgatta tggagaatat
    23881 tccccgtgaa gccctgctgc ttccctttac gttactggtt ctgacctttg tactgcgcgc
    23941 atgggtggtc tggttacgcg aacgggtggg ttatcacgcc gggcagcata tccgctttgc
    24001 catccgccgt caggttctcg accgtctgca acaagcaggg ccagcgtgga ttcagggtaa
    24061 acctgcgggg agctgggcga cgttggtact cgagcaaatt gacgatatgc atgattacta
    24121 tgcacgctac ctgccgcaaa tggcgctggc agtgtcggtg ccgttgctga ttgtggtggc
    24181 gatcttcccc tctaactggg ctgcggcgct cattctgctg ggcacagcac cgttaattcc
    24241 gttgtttatg gcgctggttg ggatgggggc tgccgatgct aaccggcgta actttctcgc
    24301 tcttgctcgc ttaagtgggc atttcctcga tcgcctgcgc ggcatggaaa cattgcgtat
    24361 ttttggtcgt ggtgaagctg aaattgaaag tattcgttct gcttcggaag atttccgcca
    24421 acggacaatg gaagtgctac ggctggcgtt tttatcctcc ggcattctcg aattttttac
    24481 ctcgctgtca attgctctgg tggcggtcta ctttggtttt tcctatctcg gcgagctgga
    24541 ttttggtcac tacgataccg gtgtgacgct ggctgcgggt tttctggccc tgatccttgc
    24601 gccagagttt ttccagccat tgcgcgatct cggtacgttt tatcatgcta aagcccaggc
    24661 tgttggtgca gctgacagtc tgaaaacgtt tatggaaacc ccgctcgccc atccgcagcg
    24721 tggcgaggtg gaattagcat cgaccgatcc ggtgaccatt gaggccgagg atctgtttat
    24781 cacgtcgccg gaaggtaaaa cgctggccgg accgctgaac tttactttgc cagcaggcca
    24841 acgtgcagtg ttggttggtc gcagcggttc aggtaaaagc tcactgctga acgcgctttc
    24901 tggttttctc tcatatcagg gatcattacg aatcaacggg atagaattac gcgatttatc
    24961 accagaatca tggcgtaaac atctctcctg ggttgggcaa aacccacaat taccggcagc
    25021 aacattgcgg gataacgtac tactggcgcg acctgatgcc agcgaacaag aattacaagc
    25081 agcgctggat aacgcctggg tcagcgagtt tctaccgctc ctgccacaag gcgttgatac
    25141 acctgttggc gaccaggctg cccgcctttc cgtggggcag gcgcagcgcg tggcggtggc
    25201 ccgtgcgtta ctaaatccct gttcgctatt actgttggat gaacccgctg ccagccttga
    25261 tgctcacagt gaacagcgcg taatggaggc gctgaatgcc gcctctctgc gccagacaac
    25321 gttaatggtc acccaccagt tagaagatct tgctgactgg gatgtcattt gggttatgca
    25381 ggatggccgg attattgagc aaggacgtta cgcggaatta agtgtggctg gtggcccatt
    25441 cgccacatta ctggcccatc gtcaggagga gatttaaatg cgcgctttgc taccctatct
    25501 ggcactgtat aaacgtcata aatggatgtt aagtcttggt atcgtgctgg caattgtcac
    25561 gctgctcgcc agtatcggtc tgttgacact ttccggctgg ttcctctcgg cctcagcggt
    25621 tgcgggggtt gccggactgt acagcttcaa ctatatgcta cccgctgcgg gcgtgcgtgg
    25681 cgcagcaatc acccgtactg ccgggcgtta ttttgaacgt ctggtaagtc acgacgcgac
    25741 tttccgcgtg ttgcagcatc tgcgcattta caccttcagc aaattgctgc ccctctcccc
    25801 tgccggactg gcgcgctatc gtcagggcga attgctcaat cgcgtggtgg cggatgttga
    25861 tacgctcgat catctttatc tgcgcgttat ctcgccgctg gtgggcgctt ttgtggtgat
    25921 tatggtggtg acaatcgggt taagtttcct tgatttcacc ctcgccttta ggctgggcgg
    25981 cattatgtta ctgacgcttt tcctgatgcc accgctgttt tatcgtgcgg gaaaaagcac
    26041 cgggcaaaat ctgactcatc ttcgcggaca gtatcgccaa caactgacgg cctggctgca
    26101 agggcaagct gagctgacca tttttggcgc cagcgatcgt tatcgcacgc aactagagaa
    26161 tacagaaatt caatggctgg aagcgcaacg ccgtcaatct gaactgaccg cattgtcgca
    26221 agcgataatg ctgctcattg gcgcgttagc ggtgatcctg atgctgtgga tggcgtctgg
    26281 cggcgttggc ggcaatgctc aacccggcgc gttaattgcc ctgtttgtct tctgcgcgtt
    26341 agccgcgttt gaagcactgg caccagtaac gggtgcattt cagcatctgg ggcaagtcat
    26401 tgcctctgcc gttcggatca ctgatttaac ggatcaaaaa ccggaagtca ccttccctga
    26461 tacccaaact cgtgttgccg atcgcgtttc gctgacatta cgggatgttc agttcactta
    26521 tccggagcaa tcccaacagg cacttaaagg gatttctctt caggtaaacg ccggggaaca
    26581 tattgcgatt ctcgggcgaa ccggatgcgg caaatcaaca ctgttacaac tgctgacccg
    26641 cgcttgggac ccgcaacagg gagagatttt gcttaacgat agccccatag ccagcctgaa
    26701 tgaagcggct ctacgacaga ccatcagcgt tgttcctcag cgagtgcatc tgtttagcgc
    26761 cacgctgcgt gataatctgt tactcgcctc gcctggcagt agtgatgagg ctctggcgga
    26821 gagcttgcgt cgcgttggtc tggagaagct gctcgaggat gcaggtctca acagttggtt
    26881 aggtgaaggc ggacgccagc tctccggtgg tgaactgcgc cgtctggcta tcgcccgtgc
    26941 gctgttacat gatgcgccac tggtgttgct ggatgaacct accgaaggct tagatgccac
    27001 aaccgaaagc cagatccttg aattgcttgc agaaatgatg cgtgagaaaa cggtgttaat
    27061 ggtcacccat cgacttcgcg gactctctcg tttccaacaa ataatagtga tggacaacgg
    27121 gcaaattatt gagcaaggta ctcacgcaga gctgcttgcc agacaggggc gttattacca
    27181 gttcaagcag ggtttgtaag ctattattga acgatccgac ttgcgtggag ttttgcggtc
    27241 atgcgcctgg ttcagctttc tcgccattca atagccttcc cttccccgga aggcgcatta
    27301 cgtgagccta acggcctgct ggcacttggg ggcgatctta gccctgcgcg cctgttaatg
    27361 gcttaccagc gtggtatttt tccgtggttt tctccaggcg accccatcct ctggtggtcg
    27421 cccgatcccc gcgcggtgct atggccagaa tcactgcata tcagccgtag tatgaagcga
    27481 tttcataaac gctcgcccta tcgtgtcacg atgaattacg cttttggtca ggtcattgaa
    27541 ggctgtgcca gcgatcgcga agaaggaacc tggatcacgc gtggcgtggt cgaagcctac
    27601 catcgccttc acgaactcgg ccatgcccac tccattgaag tctggcgtga agatgagctt
    27661 gtcggcggta tgtacggcgt ggcccaggga acgctatttt gtggcgagtc catgttcagc
    27721 cggatggaaa atgcgtctaa aacggcgctt ctggtattct gtgaggaatt tatcggtcat
    27781 ggcggtaagc ttatcgactg ccaggtcctt aacgatcaca cagcatcgct tggtgcctgc
    27841 gaaattcccc gccgggatta ccttaattat ctcaatcaaa tgcgcctcgg acgattgccg
    27901 aataatttct gggtaccacg atgcttgttt tcaccacaag aatgaatgtt ttcggcacat
    27961 ttctccccag agtgttataa ttgcggtcgc agagttggtt acgctcatta ccccgctgcc
    28021 gataaggatt ttttcgcgtc aggtaacgcc catcgtttat ctcaccgctc ccttatacgt
    28081 tgcgcttttg gtgcggctta gccgtgtgtt ttcggagtaa tgtgccgaac ctgtttgttg
    28141 cgatttagcg cgcaaatctt tacttattta cagaacttcg gcattatctt gccggttcaa
    28201 attacggtag tgatacccca gaggattaga tggccaaaga agacaatatt gaaatgcaag
    28261 gtaccgttct tgaaacgttg cctaatacca tgttccgcgt agagttagaa aacggtcacg
    28321 tggttactgc acacatctcc ggtaaaatgc gcaaaaacta catccgcatc ctgacgggcg
    28381 acaaagtgac tgttgaactg accccgtacg acctgagcaa aggccgcatt gtcttccgta
    28441 gtcgctgatt gttttaccgc ctgatgggcg aagagaaaga acgagtaaaa ggtcggttta
    28501 accggccttt ttattttgtg atatgtatga agtactttgg aagtataagt ccgtaacttg
    28561 tctcgatgta gatatactgc acaacttgtt tacagtatga gcaaacaata attatcatta
    28621 aaattcttct tgaccgtttt actccaactc cctatagtag cgccccgttg ccacccatga
    28681 ggtttggtag caaaacaatt tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa
    28741 gtgtgtatac ggcaacgtat cgggggttcg aatccccccc tcaccgccat atttaaagaa
    28801 gagctcgtac gaaagtgcga gctttttttt cgcattttat ctgtctgtac ggcagtgaac
    28861 gcgttcgcaa aggttttacc gcaccccgtc gatttctaag ctgcctgtac ggcagtgaac
    28921 tcaaatacta cacgtttagc ccgttgttaa taaagcaaaa ccatcccatt ctcctgcagc
    28981 accctttttt atccttacct tcccgcagca ctaattatca acaacttacc atcatcatta
    29041 aaaaagggtc tgaacctcct ccaccccata aacaaaaagt ccgtaacctc tttcgagatt
    29101 acggacttga ccaacctacc taacaatcag attaatgcgc tgcttccgcc ttgtgctttt
    29161 gtgcactctg gaatccgtaa gtcagctcat ttttctcttt atccagcgcg acggtgacct
    29221 gaccgccgtc caccagcgaa ccaaacagca gttcgttggc gagcggtttt ttcaggttgt
    29281 cctggatgac acgcgccatc ggacgtgcgc ccattgcccg gtcgtaacct ttctcggcca
    29341 gccagttacg cgcttcctgg ctcacttcca gagaaacacc tttctgatcc agctgaacct
    29401 gcaactcgac gatgaattta tccaccacct gatggatcac gtcggttgac agatgatcaa
    29461 accagataat gttgtcgaga cggttacgga attccggtgt aaagatcttc ttgatctcct
    29521 ccatcgcatc ggtgctgtta tcctggtgga taagaccaat ggatttacgc tcagtttcac
    29581 gtaccccggc gttggtggtc atcaccagca ccacgttacg gaagtccgct ttgcgtccgt
    29641 tgttatcggt cagcgtaccg ttgtccatca cctgcaacag aatattgaac acgtccggat
    29701 gcgctttctc gatttcgtcc agcaacagca ccgcatgtgg atgcttgatg accgcatcag
    29761 tcagcaaacc tccctgatca aaaccaacgt atcccggagg cgcaccaatc agacggctga
    29821 cggtatggcg ttccatatac tcggacatat caaagcgcag cagctcaatg cccaacgctt
    29881 tcgaaagctg taccgtcacc tctgttttcc cgaccccggt agggccggca aacaggaacg
    29941 aaccaaccgg tttatgttcg tgacctaaac ctgcacgcgc catcttaatg gcttcagtca
    30001 gcgcctcaat ggctttatcc tgaccgaaga ccagcatttt caggcgatcg ccgaggtttt
    30061 tcagggtatc gcggtcactc tgagaaacac tcttctctgg aatgcgcgca atacgggcca
    30121 ccacggactc aatatccgcc acattaacgg ttttcttgcg tttgcttacc ggcatcaggc
    30181 gtgcgcgagc gcccgcttcg tcgataacgt caatggcttt atccggcaga tgacgatcgt
    30241 taatgtattt caccgccagc tctaccgctg cacgcaccgc ttttgcggta taacgcacgt
    30301 cgtggtgcgc ttcatacttc ggtttcaggc cattgatgat ttgaacagtt tcttcgatcg
    30361 acggttcagt aatatcaatt ttctggaagc gacgcgccag agcacggtct ttctcgaaaa
    30421 tgttgctgaa ctcctgatag gttgtcgaac caattacgcg aattttaccg ctggagagca
    30481 acggtttgat caggttagcc gcatcgacct ggccaccaga cgctgcaccc gcaccgataa
    30541 tggtgtggat ctcatcaata aacaggatgc tgttagtgtc ctgctccagc tgcttgagca
    30601 acgctttaaa acgtttttca aagtcgccgc gatatttagt gcccgctaac agagaaccga
    30661 tatcgagaga gtaaatcgta cagtcagcca tcacttccgg cacatcgccc tgaacaattc
    30721 gccaggcaag accttccgca atcgcggttt taccgacacc agattccccc accagcagcg
    30781 ggttgttttt acggcgacgg cagagaacct gaatagcacg ctccagctcc ttctcacgac
    30841 caatcagtgg gtcgattccg cccacgcgcg caagctgatt caggttcgtc gtgaaattct
    30901 ccatacgttc ctccccacca gcttgttctt cgctgtttgg ctggctgcca ggatcagaag
    30961 actgtgtcgg ctcgtcttta cgcgtgccat gagagataaa gttcaccaca tcgagacggc
    31021 tgacttcatg tttacgcaac agatatgccg cctgcgactc ctgttcgcta aagatagcga
    31081 ccagaacgtt tgcaccggtt acctcattgc gaccggagga ctggacatgg aagaccgcac
    31141 gttgcagtac acgctgaaaa ctcagcgtcg gctgtgtgtc gcgctcctct tcactggcag
    31201 gcagaacggg tgtggtttgt tcaataaagg cttccagttc ctgacggagc gcaaccaaat
    31261 ccacagaaca cgcttccagc gcctcccggg cagatgggtt actgagcagc gccagtaaca
    31321 agtgctcgac ggtcataaac tcatgacggt gctcgcgcgc tctggcgaaa gccatattta
    31381 aactgagttc cagttcttga ttgagcatag gcacctcccc caatttttat gcctgcattc
    31441 aggctttttc tagcgtacac agcaatggat gctcattctc cctcgcgtac ttgttcacca
    31501 tcgccacttt ggtttctgca acctcggcgg taaagactcc gcaaatggcc ttcccctggt
    31561 agtgaacagc gagcatcaat tgcgttgcac gttctacatc ataagaaaag aatttttgta
    31621 acacgtcaat aacaaactcc atcggagtgt aatcatcatt gactaatatc actttataca
    31681 tagatggcgg ttttagcgcg tcgcgaactt tttcttccgc cagttgatca aagtccagcc
    31741 agtcgttcgt tttacccatt gtcagttatc atcttcggtt acggttatcg gtagaacgcc
    31801 ctgccgctga caagagctta tttgcctgac caatctactc cacatgatag ataactatca
    31861 tctatcgttg gcatcagcga catctgtcac attcctgtca atagcgttaa ctgcttcaaa
    31921 tttttgattc atacctgccc cctggcccct gtcaaacgct tgacggctcg ccctaattct
    31981 ctaaattgta tttctagagt tggcgaggtt ttgaacagcc ccctctctga ccccggttta
    32041 ttccatctta cttgtataag atttgcgaag gatgtcgaag catggaaaag ggtactgtta
    32101 agtggttcaa caatgccaaa gggtttggtt tcatctgccc tgaaggcggc ggcgaagata
    32161 ttttcgctca ttattccacc attcagatgg atggttacag aacgctaaaa gctggacaat
    32221 ccgttcagtt tgatgtccac caggggccaa aaggcaatca cgccagtgtt attgtgcccg
    32281 tcgaagtaga agcggcagtc gcatagctct tctgtctcat tgtgtacatc ctaaaggcaa
    32341 aatgccagcc cgatcggctg gcatttttat ctcaaaaatt actctcgtgc cagagcatct
    32401 actggatcca gtcgtgccgc atttcgtgcg ggtaaccatc caaataaaat cccggtgacc
    32461 gtcgagcaga gaaacgccag cagcagtgcc aacggtgaaa aaccaatctc ccagccgggt
    32521 aagaaaagct gcaaggtgaa agcaattaac agtgacagtg ttattcccaa cgcgccaccg
    32581 accaggcaaa ccagtacggc ttcgatcagg aactgttgca aaacatcgct tgctcgcgca
    32641 cctacagcca tgcgaatgcc aatttcccgc gtccgctcgg tcactgacac cagcataata
    32701 ttcattacac caataccgcc caccaccagc gaaatcaccg ccaccagcgt cagaaacagt
    32761 tgtaaagtac gtgtggtctt ttcaacagtt ttcaagacgc cgtccatgtt ccaggtaaag
    32821 aaatccttct ttccgtggcg cagtgaaagt aaacgcgtga gttgctgttc cgcctcggcg
    32881 ctgtcaaatc cttcttttac cctgacagta atggagttca gccacgactg gcccataact
    32941 cgcccggaca tcgtgctgta aggtagccag acacgcagca ctttactgct accaaacatc
    33001 gactgttttt cttccgccac accaatgact ctggcgggca tattgccgac cagaatcacc
    33061 tcgccaacca catctgcttt atgggggaaa agctggcggc gagtattact gtcgagaacc
    33121 acgacctgcg cacgaccgtt cagctgctcc tgattaaagg tgtttccttc actgaaggtc
    33181 atgccataga cattaaaata atcgccgctc acgccattgg cactggcagc aacatcaaca
    33241 ttgttataac gcaggcgcag gttttgcgag acggcaggtg tggctgaggc gacccacggt
    33301 tgtttttgga tggcgattaa gtcgtcgtac ttcagcgcct gctgatattg cggatcgtca
    33361 tcgccaaaat ctttcccggg atagacatca atagtattcg taccaataga acgaatatcc
    33421 gccagcacca tttgtttggc ggcgtcaccc accacgacaa tggaaaccac cgacgcaata
    33481 ccgataataa tccccagcat ggtcagtaaa gtacgcattt tattcgctgc cagcgcccgc
    33541 catgccatcg tcagcgcctc gttaaaaccg ctgacaaact gccgccagcc agacaccgtg
    33601 ttgacaacag gctccgtccc gccagcaaca ttcacttttt caatggcggg aggattgcgc
    33661 acaatttcgc cgtcgcgaat ttcgatcacc cgctcggcct gagcagcgac ctgcggatcg
    33721 tgggtgacga taatcaccgt atgcccacga tcgcgcagct gatgcaggat cgccatcacc
    33781 tcttcgccag aatggctgtc cagtgcgccg gttggttcat cggcaagaat tacctgacca
    33841 ccgttcatca atgcccgcgc gatgctgacg cgctgttgct gaccacccga aagctgtgcc
    33901 ggataatact ctgtacggtc ttccagcccc agccgttgca gcaactcctg ggcacgaagc
    33961 agtcgctgtt tccgctcaag accagcatag acggcgggta cttcaacgtt ctgctcggcg
    34021 gttaaatgcg aaagtaaatg gtaacgctgg aaaataaagc cgaaatgctc gcggcgcagt
    34081 tgcgccagcg catcggcgtc cagtgtggca acatcctgac cggcgacgcg ataggtgccg
    34141 ctggtggcct tatccagaca gccgagaata ttcatcaggg tcgatttacc ggaacctgaa
    34201 gcgccaacaa tcgctaccat ctcaccggca taaatatcga gggtgatgcc cttcagcacc
    34261 tcaacctgct catcaccggc aggatagctg cgacgaatat cctttaattc gagcaaaggc
    34321 gtcattgtgc agctcctggt ttggcctcac caatcaccac ttcatcgccc gcttcaagcc
    34381 ctttgacaat ctcaacatcg gtatcgttac gtgcgccaat cgtcacttca cgctcgcgtg
    34441 tttcaccatt acgcaacaat ttgactttat aacgattatc gccaaccgga tcgcctaacg
    34501 ccgacagagg gatcgtcagc acatttttca catcggtgag ctgaatatgc acttgcgcag
    34561 tcatatccag ccgcagcaaa ccattggggt tggggacttc aaaacgggcg taatagaaaa
    34621 tagcgtcgtt aaccttttcc ggcgtcggta gtacatcctt gatttgcccc tcgtagcgcg
    34681 tcagtggatc gccaagtacc gtaaaccagg ctttttgccc cggcttcagg tggattacat
    34741 ccgcttcaga aacctgcgct tttaccagca tggtgctcat atctgccagc gtcagaatgt
    34801 tcggtgcttg ttgtgcggca atcaccgtct ggccttgcag agtggtgatt tgcgtgactt
    34861 ccccggccat cggggcaacg atgcgagtgt aatcgagatt ggttttagcc gtatcgagag
    34921 aggcctgatt gcgcttgatt tgcgcgtcaa tggtgccaat ttgcgcctgt ttcacagcca
    34981 tctccgtcgc ggcggtgtcg agatcctgct gtgaaaccgc ctgcgtttgt gccagacgtt
    35041 gctgacggga atacgtcacc cgcgccagtt tcagctccgc ttccgcctgc tgccgctgcg
    35101 cacgtagctc catcagcgtt gcttcgacct ccttaatctg gttttcagcc tgttcaggat
    35161 caataacccc taaaagctgg tcttttttta ctttatcgcc aatcgccacc gacagagttt
    35221 tcaactgacc gctgacctgc gcgcccacgt caaccttacg cagcgcgtcc agctttccgg
    35281 tcgccagcac gctttgctgt aaatcaccgg ggcgcacaat cagtgtctga taagtcggca
    35341 cgggtgcgtt aagaattctc cataacgtaa tcagtccggc gatgactatc accagcgcaa
    35401 taacgtaacg cttcttcacg gttttccgct ttttcataaa tattctgaat actccatatg
    35461 ccaatccggg tcattaactt caacgaaata tcaacaaaac cctaaaattt acgttagtaa
    35521 tcacactatt gatgtttggt taagatacgc aatggtgaaa tcgacgtcat gtataaccat
    35581 tgatttcatg aatatgtcgc agctaactga acggaccttt acgccatctg aatctctcag
    35641 cagcctgtca ctttttctta gtctggcacg tggacagtgt cggccgggta aattctggca
    35701 tcgccgtagt tttcgccaga aatttttgct gcgctcgttg attatgccgc gtttaagcgt
    35761 tgagtggatg aacgaacttt cccactggcc taatctcaat gtgttgttaa cgcgccaacc
    35821 gcgactgcct gtgcgtctgc atcgccctta ccttgcggcg aatcttagcc gtaagcaatt
    35881 gctggaggcg ttacgttacc attatgcgtt actccgcgga tgtatgtcgg cggaagaatt
    35941 cagcttatat ttgaataccc ccgggctgca actggctaaa ctggaaggca aaaacggcga
    36001 gcagttcacg cttgagctga ccatgatgat ctcaatggat aaagaaggtg acagcacaat
    36061 cctgttccgc aacagcgaag gtattcctct ggcagagctc acttttaccc tgtgtgaata
    36121 tcaggggaaa agaacgatgt ttattggcgg actgcaaggc gcaaaatggg aaatcccaca
    36181 tcaggaaatc cagaatgcga cgaaagcctg ccacgggcta tttcccaaac gcctcgtgat
    36241 ggaagcggcc tgtctgtttg cccaacgttt gcaggtagag cagattattg ccgtcagcaa
    36301 tgaaacgcat atttaccgca gcctgcgtta tcgtgataaa gaaggcaaga tccatgccga
    36361 ttacaacgct ttctgggaat cggttggcgg cgtatgtgat gctgaacgcc attaccgcct
    36421 tccagcacag atagcacgaa aagagattgc cgaaatcgcc agtaaaaaac gggctgaata
    36481 ccgtcggcgc tatgagatgc tcgacgctat tcagccacaa atggccacga tgtttcgcgg
    36541 ttaatccgcg cgaccgcgcg ccagccacag cacgcgtgag aacatttttt tcagcagcgt
    36601 cggtacggag tccacaccac gacgtccggc ctccattgcc acttcaatgg caagatcggg
    36661 tttggaagag ctatggatcg ctttcgagat aattttgcgc agattcatcg gtacattttc
    36721 cgggatttgc gccacgcggt ggaacacatc ggaaaatccc tggcgataca taaaatgttc
    36781 catatccagc gccggtaacg ccgttaaatg ttctcgttcg gcttcccgat cgttattcaa
    36841 caggctgcgt accgtagcgg catatttctt ccctgcttca tcgccatcga ccagtacatg
    36901 ccattcaatc cccattcggc gggcaaattt aaccagtggc tttagcccgg actgggcaaa
    36961 ctcaatgacc ttgatccctt cggcatcgaa atggtgtcca cactggcgcg ccagttcatt
    37021 gataacccag gtttccgttt ccccttccac cagcaaccag cagcgagcaa acagcgatga
    37081 cggacggtta aaacgaatgt gaaaggatat gcgtcggcta tcttcggtac tcaagccact
    37141 cggccccaga cgccaggcgg caacgcgcga ggactcacgt accagtcggc aaacatgctc
    37201 taccggcgtt aacgaaagca actcacccga gttggtggtg gcaatgcgct gcaatggcag
    37261 aagattcaac agatgccagg caactgaaag cataatgggg tgtaaacggg tttctggatc
    37321 ttcaattagc aataacggac gcgcgtcttt atccagtctc aatgtgcctt ttgcctgcaa
    37381 taaagtagca aacaggccga gcaaaataac ccgatacgag cgccctccag gtcggtcaat
    37441 catccggttg atgatgtcca gatagcgcca gcttcgttgc tcattgctgg cccgccgccg
    37501 cattaaacga tatcgcgcct gtccagcccc ctgctcagag aaataatgct caagcaactg
    37561 caccattgcg gaaagtccct gacgaatctg tccatcagag agattttgcg gatgtgagga
    37621 caactcacgg gcgaggaaat cgagctggcg cgcggtgact tccacattgg gaacatttgg
    37681 caccgtgccg ttacgaatac ggcgcataaa acgggcatca cgcaagcgca gcaccggcat
    37741 taaacgcacc agatggcgtg cctgatcgtt aatatcctcg acatcaatcg gatgtccgtc
    37801 tttatcgaga aaactgcgca gtgtcatcac gctgccgtct tccgcactct ccccttccag
    37861 acgataaaaa atacggtgat agccatcggt gcatggcgtc cagcacgctt ccagcggccg
    37921 ataacggcga acccgatgtc ggcctggcag cgattcgcgg aaggtcaaaa taatatgcag
    37981 atgatgttct cgcccgttga tatctcccgg cgggaaccag aaatcgtcgc gctcaaaatg
    38041 gtagagatct gattctggcg atagcagcag ggttaaggcg tccagcaagc tggatttacc
    38101 ccacgcgttc tccccaatca ggacgttgtt ttgttcgagc atcaacgaca aacggttgat
    38161 accgcgaaaa cccacaattt caacgcgctc aagaatcata tcccctccag aaatatgtga
    38221 cttatccttt acgctggcag tataacggga aaggccttgc taatacgtga tgacatcaca
    38281 tttaagcatg gcaaatgtct gaaaaatgag cgaataatcg cgttatactc cctcttgctg
    38341 ataagtctgc aaaaacaaca aaatcaaata attagcatga taaattattt tttattacgc
    38401 c
//
LOCUS       DAZKGS010000027        36014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKGS010000027 DAZKGS010000000
VERSION     DAZKGS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(197..1426)
                     /locus_tag="ACHOQY_001399"
     CDS             complement(197..1426)
                     /locus_tag="ACHOQY_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9677533.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNVLDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            1414..1596
                     /locus_tag="ACHOQY_001400"
     CDS             1414..1596
                     /locus_tag="ACHOQY_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677534.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            1603..2508
                     /gene="tqsA"
                     /locus_tag="ACHOQY_001401"
                     /pseudo
     CDS             1603..2508
                     /gene="tqsA"
                     /locus_tag="ACHOQY_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(2533..3921)
                     /gene="pntB"
                     /locus_tag="ACHOQY_001402"
     CDS             complement(2533..3921)
                     /gene="pntB"
                     /locus_tag="ACHOQY_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9677535.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3932..5464)
                     /gene="pntA"
                     /locus_tag="ACHOQY_001403"
     CDS             complement(3932..5464)
                     /gene="pntA"
                     /locus_tag="ACHOQY_001403"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9677536.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            5988..6932
                     /gene="ydgH"
                     /locus_tag="ACHOQY_001404"
     CDS             5988..6932
                     /gene="ydgH"
                     /locus_tag="ACHOQY_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9677537.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7118..8500
                     /gene="ydgI"
                     /locus_tag="ACHOQY_001405"
     CDS             7118..8500
                     /gene="ydgI"
                     /locus_tag="ACHOQY_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9677538.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8537..9259
                     /gene="folM"
                     /locus_tag="ACHOQY_001406"
     CDS             8537..9259
                     /gene="folM"
                     /locus_tag="ACHOQY_001406"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9677539.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9256..9591)
                     /gene="ydgC"
                     /locus_tag="ACHOQY_001407"
     CDS             complement(9256..9591)
                     /gene="ydgC"
                     /locus_tag="ACHOQY_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9677540.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9720..10439
                     /gene="rstA"
                     /locus_tag="ACHOQY_001408"
     CDS             9720..10439
                     /gene="rstA"
                     /locus_tag="ACHOQY_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9677541.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10443..11744
                     /gene="rstB"
                     /locus_tag="ACHOQY_001409"
     CDS             10443..11744
                     /gene="rstB"
                     /locus_tag="ACHOQY_001409"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9677542.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11820..12749
                     /gene="tus"
                     /locus_tag="ACHOQY_001410"
     CDS             11820..12749
                     /gene="tus"
                     /locus_tag="ACHOQY_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9677543.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12746..14149)
                     /gene="fumC"
                     /locus_tag="ACHOQY_001411"
     CDS             complement(12746..14149)
                     /gene="fumC"
                     /locus_tag="ACHOQY_001411"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9677544.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(14292..15938)
                     /gene="fumB"
                     /locus_tag="ACHOQY_001412"
     CDS             complement(14292..15938)
                     /gene="fumB"
                     /locus_tag="ACHOQY_001412"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9677545.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            16137..17312
                     /gene="manA"
                     /locus_tag="ACHOQY_001413"
     CDS             16137..17312
                     /gene="manA"
                     /locus_tag="ACHOQY_001413"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9677546.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            17413..18921
                     /gene="ydgA"
                     /locus_tag="ACHOQY_001414"
     CDS             17413..18921
                     /gene="ydgA"
                     /locus_tag="ACHOQY_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9677547.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18966..20231)
                     /gene="uidC"
                     /locus_tag="ACHOQY_001415"
     CDS             complement(18966..20231)
                     /gene="uidC"
                     /locus_tag="ACHOQY_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP9677548.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(20270..21643)
                     /gene="uidB"
                     /locus_tag="ACHOQY_001416"
     CDS             complement(20270..21643)
                     /gene="uidB"
                     /locus_tag="ACHOQY_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9677549.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(21640..23451)
                     /gene="uidA"
                     /locus_tag="ACHOQY_001417"
     CDS             complement(21640..23451)
                     /gene="uidA"
                     /locus_tag="ACHOQY_001417"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9677550.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(23840..24430)
                     /gene="uidR"
                     /locus_tag="ACHOQY_001418"
     CDS             complement(23840..24430)
                     /gene="uidR"
                     /locus_tag="ACHOQY_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9677551.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(24651..25418)
                     /gene="hdhA"
                     /locus_tag="ACHOQY_001419"
     CDS             complement(24651..25418)
                     /gene="hdhA"
                     /locus_tag="ACHOQY_001419"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9677552.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(25530..26558)
                     /gene="malI"
                     /locus_tag="ACHOQY_001420"
     CDS             complement(25530..26558)
                     /gene="malI"
                     /locus_tag="ACHOQY_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9677553.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            26733..28325
                     /gene="malX"
                     /locus_tag="ACHOQY_001421"
     CDS             26733..28325
                     /gene="malX"
                     /locus_tag="ACHOQY_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9677554.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            28335..29507
                     /gene="malY"
                     /locus_tag="ACHOQY_001422"
     CDS             28335..29507
                     /gene="malY"
                     /locus_tag="ACHOQY_001422"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9677555.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            29611..30612
                     /gene="add"
                     /locus_tag="ACHOQY_001423"
     CDS             29611..30612
                     /gene="add"
                     /locus_tag="ACHOQY_001423"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9677556.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(30647..31687)
                     /gene="ydgJ"
                     /locus_tag="ACHOQY_001424"
     CDS             complement(30647..31687)
                     /gene="ydgJ"
                     /locus_tag="ACHOQY_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9677557.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            31930..32055
                     /gene="blr"
                     /locus_tag="ACHOQY_001425"
     CDS             31930..32055
                     /gene="blr"
                     /locus_tag="ACHOQY_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9677558.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            32328..32543
                     /gene="ydgT"
                     /locus_tag="ACHOQY_001426"
     CDS             32328..32543
                     /gene="ydgT"
                     /locus_tag="ACHOQY_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9677559.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            32629..33069
                     /gene="ydgK"
                     /locus_tag="ACHOQY_001427"
     CDS             32629..33069
                     /gene="ydgK"
                     /locus_tag="ACHOQY_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9677560.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            33146..33727
                     /gene="rsxA"
                     /locus_tag="ACHOQY_001428"
     CDS             33146..33727
                     /gene="rsxA"
                     /locus_tag="ACHOQY_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9677561.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            33727..34305
                     /gene="rsxB"
                     /locus_tag="ACHOQY_001429"
     CDS             33727..34305
                     /gene="rsxB"
                     /locus_tag="ACHOQY_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9677562.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            34298..>36014
                     /gene="rsxC"
                     /locus_tag="ACHOQY_001430"
     CDS             34298..>36014
                     /gene="rsxC"
                     /locus_tag="ACHOQY_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9677563.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLE"
BASE COUNT         8722 a         9365 c         8769 g         9158 t
ORIGIN      
        1 tcatgccttt tttctcgcta tttaaagagt gattcgctag tgtataaaaa accagtaaca
       61 cattaaaaat cagctactta taaatctgta cactttttcg cctcttgttc gtagttgttc
      121 cgcattgttg ctcattgttg cacgttgtat ctgttcgcat atccagtatg cgcatactga
      181 aaaaatacga aaaaaattat tttcttctgg ctactggtag cgtggttacg ttttcatgtg
      241 ttcccgccag tatccctaac cgctccatcc acatatccag cacattgcgt ttagcatcca
      301 gataacggga atgattataa actcgctgca ttcctggcat ctggtggcct gtaagctgct
      361 ccacgacatg cggatcaacg cctaaatcgt tcagcatggt tgtaaaggtg cgccggatgt
      421 catgcagtga ccagtgagga tgattaagcc tcctgtgcgc taatcttcca tactgcgata
      481 cgctggcctc ctgtttcact tcccccagca ataagcccgt gtgcctgttc tgctccacca
      541 gctgcgtgac gaacggcagg atcgcttccg gtatgggccg gaatattgcg accttcgttt
      601 tgctgtgctc cttcggaacg gtccatagca tttccgtaaa atcccactcc ccgatctccg
      661 atagcctcag ttctaccgtc ctggctccga agacaatcag gaggcggatt aacgcgacgt
      721 agtaagggga aaatattttt ttgtccagtg cctgcaataa ttcgccaagt tctttgttac
      781 ttaagacacg ttcgcttata tccggttttt tcccaacgtc cgccacgttc agatcgtcca
      841 gaacgttgct gattgcatag cgccgcctac ggcagaactt aagcgcctgt ttgcacgcct
      901 gtagcacgaa tccggcagta acaggcgttc gctttgccac ctggtcaaaa caggccagcc
      961 agtgccgtag ctcgcattta tccagcggca tagcaccaat ctgctgtatt acgtgattat
     1021 taagtcgcct tttcagggcg atataatcca cgcggttttc ctttacgtaa tactcaagcc
     1081 agtaggtgag cgcatcgcca accgttacgg gctttaacgc ttcctgtacg gtgtaattca
     1141 tctcatgacg tggatttttc ccctcagcca gccatgtgcg acactgtgcg gctttttccc
     1201 tggctgcttt caggctcaga tcaggataac ttcccagctt aatgcgttcg ggtcgtgtct
     1261 cccttcccgt tccggccctg tatgtgaaat accaggtcaa tttccctgat gttaaatatt
     1321 tcacgctcag gtttccgcca tcactataaa acgtgttttt ctccgctggc ttaccatgaa
     1381 gtttccttag caaggtatcg ctcagtttgt tcattgctct gctcacgttt taccccctct
     1441 ttacgaaaaa gtgactacac cagtgactac accgatcgtt gcacaaaggg ctacaacgtg
     1501 aaacaagctg gaacaaggaa aatcacaaat ctgttgataa tcaaaaacat aatgcacagc
     1561 ctgaaacatt atgaaacagc aaaaaacact aaatgataaa atgctgttat tcttaacccg
     1621 ctggtgcaac acatggtccg ctggcgagtg ccgcgtgtac tggcggtgtc gattttgatg
     1681 accatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc gctcaacgag
     1741 ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca agctcttgag
     1801 ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc agctggcgca ttacattgat
     1861 ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa tgccatgtca
     1921 tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc acaattgccc
     1981 ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat tcaacgtgca
     2041 attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat caccggcctg
     2101 gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg gggattgctg
     2161 gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat cccccctatc
     2221 gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc gggatatctg
     2281 ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg tgggctgggg
     2341 ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt aggaccggtg
     2401 ggtatgctgc tttccgtgcc gttaacaatt attgtcaaaa ttgcgcttga acaaacagcg
     2461 ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg gcctccgcag
     2521 aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg cgtcaccaaa
     2581 cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat agccagtgtt
     2641 catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca caggcatacc
     2701 agcaatagga ctcttcggat catcctgcgc cgccgggtta accgtatcgt tagcaccaat
     2761 caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt ccagcacgat
     2821 gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca aacgccccgc
     2881 aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct cggtaatttc
     2941 agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa tgatcactga
     3001 atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt gctcgcccac
     3061 ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa taacgctgat
     3121 aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcgcccg aagaaccgac
     3181 cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc
     3241 ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac caccgatgga
     3301 ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca atgccagcac
     3361 ttgcaggccg acgctatccg tgcgaacaaa tacaatcagc agcaggaagg aaacgaccag
     3421 agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg aaatcttgcc
     3481 acacagtttg ccgaacgcca ccaccgaacc cgtgaacgtt accgccccga tgaagatacc
     3541 gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg cgtcatgatg
     3601 cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctat gcaggatcgc
     3661 caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac caattgcccc
     3721 accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc caaaaatggt
     3781 tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc gagacgtttc
     3841 atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa tgtatgcagc
     3901 tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt ttcagcatgc
     3961 gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc gcgataaaac
     4021 taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca acaataatcc
     4081 ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcatgcgat acattccaca
     4141 ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca agaaactctt
     4201 tcggcgcaac gcttgccagc caaccgaaaa ggatgattgc cagcgccatc aatgcgtatt
     4261 tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc gctttttgtg
     4321 ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt tcgcccgcac
     4381 ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata ttgccgtctt
     4441 tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc tgtgaggatt
     4501 gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca ttttccgtag
     4561 tgaagatttc acccggcacg gtgtattcac agttgccacc gttttgggct gccaggtcga
     4621 caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc ttcggcgctg
     4681 gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc tgggcggcaa
     4741 agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag ccatcgccgc
     4801 tgcccgcttc ctctttaaaa tccagctcga ggaattccgc gcccatactt tgaacttgtt
     4861 ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg tttgctgcgc
     4921 caatggcggc cagacctgca acacccgcac caatcaccat cacttttgcc ggtggcactt
     4981 tcccggccgc agtaatttgc ccggtaaaga agcgcccaaa ttcatgtgcc gcttcaacaa
     5041 tggcgcgata accggcgatg ttcgccatcg agcttagtgc gtccagcgat tgtgcgcgtg
     5101 agatacgcgg cacagaatcc atcgccatca cggtcacgtt acgttccgca agtttttgca
     5161 ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccca ggattcagta
     5221 acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac tgccagacgc
     5281 tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca aaacttgcca
     5341 gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc tgttccactg
     5401 tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt atgccaattc
     5461 gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca ggagccacac
     5521 aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa caaacgccct
     5581 ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt cggcgtgaac
     5641 tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa cgccggataa
     5701 agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc acgaatcact
     5761 acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat gctgatacgg
     5821 ctgtttttta agcatagacg gtcatttgag caggattaaa attggcttaa ggaatgtgat
     5881 atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac gtttctcgtt
     5941 aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg aagcttaaga
     6001 acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac gcagcaacag
     6061 aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg gttaccggtc
     6121 gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat aaagaaggtg
     6181 ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac tggcgtgtgg
     6241 tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat cgcgtaatta
     6301 acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt gacacggtca
     6361 ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc aaagcagcaa
     6421 aagcgaaagg tgcctactct ttctacattg ttcgtcaaat cgatgccaac cagggcggca
     6481 accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc gtccagagcc
     6541 cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc ggtggcgaag
     6601 ccgcgaagaa agttgagatc ccaggtgttg cgactaccgc atcaccaagt tctgaagtcg
     6661 gtcgtttctt tgaaacccag tcatcaaaag gcggacgtta cactgtcacg cttcctgacg
     6721 gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg ttcgacagca
     6781 tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt gcgaaacgtg
     6841 ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa cgtggtaata
     6901 acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat aaggcggctt
     6961 gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat gcagacacat
     7021 ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt tgccctaata
     7081 tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac tgggactgag
     7141 cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca gtctgccgca
     7201 aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta ttactggcgc
     7261 tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc ccgaacttga
     7321 cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct tttgttccgc
     7381 atggggatac tggctgtgcg cagtcatcgc caacgtctcc tatctggtga tcgttttttc
     7441 cgcgttaagc ttttttacgg acacgccgga attgcgcctg ttcggcgatg gtaatacctg
     7501 gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga tcctgcgcgg
     7561 ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt tgccgctggg
     7621 tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc tcgacttcac
     7681 cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc tgatcaccct
     7741 gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc gtaataaacg
     7801 tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt acttgctggt
     7861 aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc gtaacccgtc
     7921 aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca tcgctgccgg
     7981 tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg cagaagttcc
     8041 gttcctggct gccactcata aagcattccc acgcattttt gcgcgccaga acgcacaagc
     8101 tgcgccatcc gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc tggtactcat
     8161 ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa tgattctggt
     8221 gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac tgcataaagc
     8281 ggtaggtgtc ggtgcctgca tttatggctt atggttattg tatgcctctg gtccaatgca
     8341 cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt atgcgcgcaa
     8401 aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg gcatgctgtt
     8461 aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatacc atcgttggat
     8521 agcaaaggag ataacgatgg gtaaagccca gcccttgcca atattaatta ctggcggagg
     8581 tcgtcgcatc ggcctcgccc tcgcctggca tttcattaat caaaagcaac cggtgattgt
     8641 cagctatcgg acgcactatc cagccattga tggactgatt aaggcaggtg cgcagtgtat
     8701 tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag tactaaaaag
     8761 cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg aaaaaccagg
     8821 tacaccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata ccccatacct
     8881 gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca gcgatatcat
     8941 tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catgttgcgt atgctgcaag
     9001 caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac cggaagtgaa
     9061 agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg ccgaatatcg
     9121 acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag aagtgatcga
     9181 cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtttcc cacttgatgg
     9241 cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc atgcgcttat
     9301 cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc cggtgaaata
     9361 ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa aaatgatggt
     9421 tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga taagcgcaaa
     9481 ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg ctaaaacacc
     9541 aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca tctttttacc
     9601 ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt tagaaacgat
     9661 tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc gtgaatgtta
     9721 tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt gccgcgtacc
     9781 tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc gaagaaacca
     9841 ttttgcgaga aagtccggat ttggtgttac tcgacatcat gctaccaggc aaggacggca
     9901 tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt ctaacctctc
     9961 tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac tatattctca
    10021 aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt cagaatgagc
    10081 aagccacact gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa gccctgcatt
    10141 tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac actgaaatct
    10201 cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc gggcaaatca
    10261 tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga ctggatcgta
    10321 gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc gcagaacctt
    10381 atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca tgggaataag
    10441 cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc cttgtgatgt
    10501 ctctgctggt tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa cagtcgctgg
    10561 atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag atccccccac
    10621 acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat ctgcgtgtcg
    10681 agccactgag taaataccat cttgatgata tttccatgca ccgactgcgt ggcggcgaaa
    10741 ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc cactacgtgc
    10801 tggcagttgg tcctgttcct tatctttatt atctccatca gatgcgattg ctggatatcg
    10861 ccctgatcgc ttttattgct atttccctcg cctttccggt gtttatctgg atgcgtccgc
    10921 actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat gggcatctca
    10981 atgagcgtat ccactttgat gagggttcga gctttgaacg acttggtatc gcatttaacc
    11041 agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt gacggtatcg
    11101 ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg agcgataacc
    11161 tgagcgccgc cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa gctttaattg
    11221 aagagctgct gacttatgcc cgactcgatc gcccacaaaa cgagcttcat cttagcgaac
    11281 cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta acgcccgata
    11341 aaacggtacg gataaaaacg ctcgtgcaag gccattatgc ggcgttggat atgcgcttaa
    11401 tggagcgcgt gctggataat ttgctcaata acgccctgcg gtactgccat tcaacggttg
    11461 aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat gatggcccag
    11521 ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc gatcccagcc
    11581 gggatcgctc aaccggcggc tgcgggctgg ggctggcaat tgtccactct atagcactgg
    11641 caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgctcgc ttctcgttta
    11701 gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactac gcgcacgatg
    11761 gtcaagtcac cacgactgtg ctataaaata agtatgttgt aactaaagtg gttaatatta
    11821 tggcgcgtta cgatctcgta gaccgactca acactacctt tcgccagatg gaacaagagc
    11881 tggctacatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc gtgttctctt
    11941 tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag gtaaaacaac
    12001 atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta tttattcaac
    12061 aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg ttgtgttacc
    12121 aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat tcagcacatc aataaactca
    12181 agaccacgtt cgagcatatc gtcacggttg aatcagaact ccccaccgcg gcacgttttg
    12241 aatgggtgca tcgtcatttg ccggggctga tcacccttaa tgcttaccgc acgctcaccg
    12301 ttctgcacga ccccgccact ttacgctttg gttgggctaa taaacatatc attaagaatt
    12361 tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca cgcagtgtcg
    12421 caccgtggac gcgcgaggag tggcaaagaa aactggagcg agagtatcag gatatcgctg
    12481 ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag ccgattgccc
    12541 gcgtctggta caaaggagat caaaaacaag tccaacacgc ctgccctaca ccactgattg
    12601 cactgattaa tcgggataat ggcgcgggcg tgccggacgt tggtgagttg ttaaattacg
    12661 atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt ttgatcattc
    12721 cacggctgca cctgtatgtt gcagattaac gcccggcttt catactgccg accatctgtt
    12781 ctggccgtac ccagctgtca aactcggctt cgctaagata ccccagcgca agggccgcag
    12841 cttttaaggt cagcccttct ttatgcgctt ttttggcgat ctccgcggct ttgtcataac
    12901 caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat tgattgattc
    12961 gctcacgatt cggttcaata cccactgcgc agtgtttgtt aaaactttcc atgccatctg
    13021 ccagcaagcg caccgattgc aggaaattgt ggatcaccat tggacggaag acgttcagtt
    13081 caaagttacc ggaagcgccc cccatgttga tcgccacgtc gttccccatc acctgacagc
    13141 agagcatggt taatgcctca cactgtgttg gattcacttt ccccggcatg attgagctgc
    13201 ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca gaggccagcc
    13261 agcggacatc attggcgatt ttcatcagtg acacagccaa ccctttcaac gcgccgtgcg
    13321 cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg gtaacaaacg
    13381 gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatactcc ggatgggtat
    13441 ttagtccagt acccaccgct gtaccgccaa gagccagttc cgctacgtga ggcaggctgt
    13501 attcgatatg tttgagatta tgctcgagca tcgctaccca gccggaaatc tcctgcccta
    13561 gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat tttgacgata tcggcaaatg
    13621 cacgcgattt ttcactcagt gtctgtgtca gggttttaag ctgcggaatg agttgcttgc
    13681 gcagcgccag cagcgccgca acgtgcatcg ccgtcggaaa gacatcgttg gaactttggc
    13741 ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg ccgccgagta
    13801 attcactggc ccggttagcc agcacttcgt tcatgttcat gttactttgc gtgccggagc
    13861 cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt acttcatccg
    13921 ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct tcattaactt
    13981 ttgccgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc attttctccg
    14041 tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc ttatctgccg
    14101 ggacatcaat cgcccccatc gaatcttttt cgctgcgtac tgtattcatg gcctgctcct
    14161 cacctgttta attttttctt tctgttttgc tttcgttaag caacttttag ctcacttatt
    14221 atttactatt tgataacaaa tgtttggtct ttcgtgccat gtaaaaaaac cgccccgaag
    14281 ggcggctctg tttatttcac acagcgagtg cactgtgtga gttgtatctg ctggaagaag
    14341 tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc aattttccag
    14401 atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat acttccctgc
    14461 gccaatacag cggccggacc accgatactg ccaaggtaga agccgccgtg ttttttacag
    14521 gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact tccgccctgc
    14581 gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc aagagaaccg
    14641 gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg atctttaatg
    14701 tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg agcaatatca
    14761 cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag aaacgggata ctgcgacaac
    14821 tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc gccttctccc
    14881 gctttacgca gctcttcagg gatatattta cctggattat gttccagttt ttcgatccag
    14941 atcccctgac ggttgatctt cgctttgata ttacggtcag cagagcagga aacacccata
    15001 ccaaccggac aggatgcgcc gtgacgtggc aggcgaatca cgcgtatgtc gtgagcgaag
    15061 tatttaccac caaactgcgc acccagaccg agattttgcg cttcgatcag caattctttt
    15121 tccagttcca catcgcggaa tgcctgaccg tgctcattcc cttccgttgg cagttcgtca
    15181 tagtatttag cggaagccag tttcactgtt ttaaggttag tttctgcaga agttccacca
    15241 attacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg catcttctca
    15301 accaggtaat ttttcagttt ccccggcgtt agtaacgctt tggtttcctg atagagatac
    15361 gtcttgtttg ccgaaccacc acctttggcg atacagagga atttgtactc gtcgccatca
    15421 acggcataaa gatcgatctg cgctggcaga ttggtgccgg tattcacctc tttatacata
    15481 tccagcggcg cgttttgcga gtagcgcaaa ttatcttcga tataagtgtt atagacaccg
    15541 cgcgccagcg ccgcttcatc acagccaccg gtccatacac gctgcccttt tttaccaaca
    15601 ataatcgcag tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc gatgtcggag
    15661 ttacgcagga attgcagcgc cacatattta tcattttcgc tggcctccgg gtcacgcaga
    15721 atgtcggcca cctgttgttg gtgagccgga cgcagcataa acgacgcatc atgaaacgcc
    15781 tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg cccttcaaat
    15841 tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc ttttttgagt
    15901 ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact tactgcctgg
    15961 tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa caatatccta
    16021 cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag gttacttccc
    16081 gtaggattct tgctttaata gtgggattaa tttccacatt aaaacaggga ttgatcatgc
    16141 aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg ttgactgaac
    16201 tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg ggcgcacatc
    16261 cgaaaagcag ttcacgagtg cagaatgccg ccggagatat cgtttcactg cgtgatgtga
    16321 ttgagagtga taaatcgact ctgctcggag aggccgttgc caaacgcttt ggcgaactgc
    16381 ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt catccaaaca
    16441 aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtatcccg atggatgccg
    16501 ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg ctgacgcctt
    16561 tccttgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctactc cagccagtcg
    16621 caggtgcaca tccggcaatt gctcactttt tacaacagcc taatgccgaa cgtttaagcg
    16681 aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg ctggcgattt
    16741 taaaatcggc gctcgacagt cagcagggtg aaccgtggca aacgattcgt ttaatttctg
    16801 aattttaccc ggaagacagc ggcctgttct ccccgctgtt actgaatgtg gtgaaattga
    16861 atcctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg caaggcgtgg
    16921 cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg cctaagtaca
    16981 ttgatattcc ggaactggtt gccaatgtga aattcgaagc caaaccggct aaccagttgt
    17041 tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattccagtg gatgattttg
    17101 ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag agtgccgcca
    17161 ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag ttacagctta
    17221 aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc aaaggccacg
    17281 gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt aacatctctt
    17341 gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga tttcatttta
    17401 tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc gctaggcgta
    17461 gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct cgaagacatg
    17521 gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct ggaagtgagt
    17581 tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt gaaacccatt
    17641 gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa cgaatcggtt
    17701 gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc gtcgatggca
    17761 tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga tatggcaaaa
    17821 ggtgaaacgc cttttgagat taactcgcgc attggttaca gcggtgattc cagttccgat
    17881 atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt tagcggcggc
    17941 gagttccagt taaatgctga cagagacggc aaagccatct ccctttccgg ggaggcgcaa
    18001 agtggtcgga tagacgcagt taacgaatac aaccagaaag tgcagttgac ctttaataat
    18061 ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg aaaccaaaaa
    18121 ctgtcactgg aaaaaatgac catttcagtg gaaggcaaag aactggcact gctggaaggc
    18181 atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa tagccaactg
    18241 gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagcgg caagctgact
    18301 ttaaaagtcg gccagattga tggtgaagcc tggcatcagt ttagccagca atataacgcg
    18361 caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact ttatcaggag
    18421 aaagtgacgg aagccttctt tagcgccctg ccgctgatgt tgaaaggcga tccggtgatt
    18481 actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa tctgtcgctg
    18541 ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca ggaagtagat
    18601 cgttcggtta aatctctgga tgcgaaactg accattccgg tggatatggc aactgagttt
    18661 atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa actggcgaaa
    18721 cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac cttgcaggac
    18781 aataccatca ccaccagcct gcaatatact aacggtcaga taacgttaaa cgggcagaaa
    18841 atgccactgg aagatttcgt tggtatgttt gcaatgccgg cattaaatgt tccggtcgta
    18901 cccgctattc cgcagcagta attcacagac aaaacgccgg agatttttct ctccggcgtt
    18961 attttttact tcagcataaa gtcatactga atactcattt tcacatccca gtaattcagg
    19021 cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc aagaaagaaa
    19081 ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa gatacccgtg
    19141 ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat cggtgcaaat
    19201 gtcagcgtaa attgcggcgt taaacgatag ttgacgccca attgccagga ctgcattcct
    19261 tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc caccacggca
    19321 ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca cggcgcattc
    19381 caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt acgacttaaa
    19441 ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt cgggtcgagc
    19501 ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg taccgatgcc
    19561 cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga acgtgcgcga
    19621 tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat accggttgtt
    19681 gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat atttggtgtt
    19741 aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg cgaactgcca
    19801 gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc gaagcgacct
    19861 agtttaagca ggctattttc actgggtttt atttttactg cccccagggc aaagccaaaa
    19921 cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc taatttataa
    19981 acataatatg ccccgccttc aacgccaata atattggaat aataaccact attgaaatcg
    20041 agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact gggcttatcc
    20101 gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc agccagttgc
    20161 gccgcataag cagaggtaag gccagaggca gccgtcaggc aaataacggc catggccact
    20221 atttttctca tagttgcacc tttgatgttc tgattatttt tattgaatat taattagtga
    20281 tatcgctgat taattgctgc tgcacttttt tacgattatc aatttcaacc acgatttctt
    20341 tgaatttttt atccgtgagc ggataaaacc agataataac gaatgccagt agcataaatc
    20401 cgcaaggtac taaggcaatt gatgtgcgga tgcccataat aacttccggc gtttgcacct
    20461 gattggcgat atatccgctt aatcccaaaa taaaggcagg aattgaacct ccgattgcct
    20521 gaccgcattt acgggtaaat gagaatagtg aataggtgag cccttcaatt cgcacgccgg
    20581 tcaggtattc accgtattct acggtatcag cttccagtgc ccacatcaca gtcatagtaa
    20641 cgccctgacc aattgaagcg atggccaacg caaccaacgc caccggcagt gaccagacgg
    20701 aaacccagaa gaatagcaga taaccgcagg ttcccagcaa agcgccaatc aggaaggtat
    20761 tctttttacc gatcctcgcg accatccccg gcaccagcgg tgccgatgcc acagtaccaa
    20821 ccaggttttg caccagtacc agcacagtga acagcccggt atcatttaac acatagcgca
    20881 cgtagaacaa cgacgaggcg ctgaccgcga aggtcgaaat cagcacacac agcgcaccga
    20941 tgcacaacat aaacagcggg cgattccgtt tcagggtttg cagactgatc ttcaatgacg
    21001 gctgcgccac gatacgtacc acattctcac gcgtcgattt gaagcagatg aagtaaagca
    21061 ccattccggc aatcgccagc acaatcgtcc agaaatggta taccgacacc atctcttccg
    21121 ggctggagtt cttaatgctc ggtcctatca gaaatgccag gcagacaaag gttaatgaag
    21181 cggcaatccc gcgagccgcg cccagacggg cgcgggattg tggttgttgg gtcatcgcgg
    21241 tagcaagtga accataagga atattcacca ggctgtagca aagcccgagg cccatgtagg
    21301 tcaaatatgc atacaccact ttgctaccat ggctccagtc ggtcggcacc cagaatacca
    21361 gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg aattttcccc
    21421 agcgggtatt cacactgtcc accactcgtc cggcaaagac gtcggcgaag gcatcgaata
    21481 cccgcaccag taacagcatg gtgcccgccg cagcggcacc gacgccagcg acgtcggtgt
    21541 agtaactcaa taggaagagc gcccccattg cgaaggcgaa gttattggcg acgtcaccga
    21601 ggctgtagcc gacgatggtg cgccaggaga gttgttgatt cattgtttgc ctccctgctg
    21661 cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcagaaaag ccgccgactt
    21721 cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca atatgccttg
    21781 cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc tgacgcgatc
    21841 aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc acatgtcggt
    21901 gtacattgag tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga taatcggctg
    21961 atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg ccgtttccaa
    22021 atcgccgctt tggacatacc atccgtaata acggttcagg cacagcacat caaagagatc
    22081 gctgatggta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga tcggacgcgt
    22141 cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg caccttgcgg
    22201 acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt ttttgtcacg
    22261 cgctatcagc tctttaatcg cctgtaagtg cgcttgctga gtttccccgt tgactgcctc
    22321 ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta aagagaggtt
    22381 aaagccgaca gcagcagttt catcaatcac cacgatgcca tgttcatctg cccagtcgag
    22441 catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa tccagtccat
    22501 taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca agtccgcatc
    22561 ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga actgttcgcc
    22621 cttcactgcc actgaccgga tgccgacgcg aagcgggtag atatcacact ctgtctggct
    22681 tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga ggtgcggatt
    22741 caccacttgc aaagtcccgc tagtgccttg tccagttgca accacctgtt gctccgcatc
    22801 acgcagttca acgctgacat caccattggc caccacctgc cagtcaacag acgcgtggtt
    22861 acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt tcggcgtggt
    22921 gtagagcatt acgctgcgat ggatcccggc atagttaaag aaatcatgga agtaagactg
    22981 ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct gccagttcag
    23041 ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat acggcgtgac
    23101 atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat tattgaccca
    23161 cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc ctgcccaacc
    23221 tttcggtata aagacttcgc gctgatacca gacgttgccc gcataattac gaatatctgc
    23281 atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt cttgtaacgc
    23341 gctttcccac caacgctgat caattccaca gttttcgcga tccagactga atgcccacag
    23401 gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca taagggactc
    23461 ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa ttctgtgttg
    23521 cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga actgacaccg
    23581 cgatcaaaaa accaaaaact gcaataccca cagcgtgata ttgatcgcat taatctttaa
    23641 aacggcgttg acagaagcca taaaataagc aaccgaacgt tcgttcgagc atattgggaa
    23701 cgataaactc tcatgacata tggctacggt gaatattttt gggggggaat tctccgatcc
    23761 cggctagatt gtattttgta cagggaaatt aaagtatgaa acgcgggcac agttatcacc
    23821 agcccgcgaa atgagaggat caggatgcgg ttaagatacc gccaatcatc gcgcgtaatc
    23881 cctgagcaaa cgacgcttca cgcgccagat cttccgcttc gatatcggcc agtacaccgt
    23941 aggtcagatc cagcagtaaa cgtgaagtca ttgacgtgtt gatgtctggg cttatctcgc
    24001 ctttttgctg ggcatcacgc atccgctcgg caacaaattc agtgatcgtc atatgcttat
    24061 ttttaagcat ggcggcgacc tgtgggttac gcatcccttc cgccataatt tcgaccacca
    24121 gcgcccgttg cccaaaggct tcatgggtga gagagacaat agactcgacc atatagtcaa
    24181 caaaatgaat cccttcaatc ggttcccgga aacgggccag cgccctctcc tggtcctgta
    24241 agataatcgc ctgaatcaag gcttctttgg agatgaaatg gtgatagagc gtcccgggac
    24301 taatggcgca ggatttacag atggctttca tcgaggcact gtgaaatcca ttttctgaaa
    24361 aaatctctct ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca gtctgcatgt
    24421 tatccatcat aaaaccatta tagttagcgt gaataactag tttaccgcgt tgtcaggtga
    24481 gcgcctatca ttgctgttga atagtgcgaa ggcacactct attcgtatac ttttcactct
    24541 gaaattggtt aaccaatagc cgtgatttgg ctgccagtta tttagcagag acgttgatga
    24601 gtttatcccg gtaaacaaca cgcacggttg tttaccggtc cgttagtgta ttaattgagc
    24661 tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct cgcagcaggc
    24721 gagcaaagga acagcgctgc gttagcaata tcttgcggtt ggcccagacg tctgatcggc
    24781 gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag ggcatcggtt
    24841 aatattgccc ccggcgcaat gccatttacc cgaatatttt tttcacccag gtcaaacgcc
    24901 atatttctga ccagatgact ggctgcagct ttagatgatg cataggaagt catgtttata
    24961 tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc atttttttcc
    25021 atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag ttcataagca
    25081 cggcgaaaat ccgccattgg catatcaaac ggtttaggtc caccgccacc ggcgttgtta
    25141 accagaatat caactttacc cagcttactg atagcaaagt ctgccagtgc agagagttcc
    25201 tgttcggaag taatatcaca acggcaggca aatgcctgac cacccagttg ttgaatttcg
    25261 tctacaacat ggttagctgc gtcggcgtta atatcactga ccaccacaga tgcgccagct
    25321 gtcgcgaatg taatggcgat ttctttacca atacctgcac ccgcacctgt gatgatggcg
    25381 cattttccgt cgagtctcag gttgtcagaa ttaaacacat aaacctcctg tgaacttcag
    25441 taaaaataaa aggcaccgcc tgtgtgccac attttttatc attaaccttg aggaaaggat
    25501 actaccaggt atagcagata aagagaggat tatttcgccg caatgagccg ggcgggaata
    25561 ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg atccgcaagt
    25621 gtgataccaa gttcccgcgc tggcgtgctg gcccaggtaa caggtatatc atcaagtgtg
    25681 gttggtgtcg catcggtaaa tgccgccagc gaaacctgtt gctcaaagta acgatcgact
    25741 ccgctttccc cgctttgccg ccctgctttg agcaaaccaa accatgcccc catcgcaata
    25801 gtttcgttat agcaaaccac ggcactgatg gtcgggttat gacgtaaaag cgccgtgata
    25861 gcttccgcgg cttgcttctg gctggaagtg cactccaaca cccaatcgct atgaaacggc
    25921 aggccaaatt ttagtagagt tgcacaatag ccccccaccc gttctgcacg ggttaatgag
    25981 gaactttgcc ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag atgctccgtc
    26041 aacaactgtg cagcctgcat gttgtccggg cgaaccgtat caacatcatc aagataactg
    26101 gcacgggaag cgaaaatcac cgggatagct ttttcttctg ccatccgtcg caggtcatcg
    26161 ctgcttcctg cagccccggc aattaccaca ccatccacac cctgattcag taacagtgaa
    26221 aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac catccgtccc
    26281 tgcgcttcca gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg cgcagataaa
    26341 tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga cgcctggcga
    26401 ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt ggcggtagag
    26461 attcgccctt tgccactaag caccagcgaa acggtactta ccgacacgcc cgcagccagc
    26521 gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta ctccctgatt
    26581 atgtgacaga taaaacgttt taccttttat ttatcttata ctcgctatta tcgttgcgta
    26641 atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg caatttactg
    26701 aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca ccgaaagtca
    26761 cgctgtggga gttcttccag cagttaggca aaaccttcat gttacccgtg gcattattgt
    26821 cgttctgcgg cattatgctc ggcattggta gttctcttag tagccacgat gtcatcaccc
    26881 tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg
    26941 gctcgtttgc ttttagtttc ctgccagtca tgttctgtat cgccatcccg ctgggtctgg
    27001 cacgcgaaaa caaaggcgta gcggcattcg ctggcttcgt cggttatgcg gtaatgaacc
    27061 tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggac gccgcggttc
    27121 tgaaagccaa taacatccag agcattcttg ggatccagtc gatcgacacc ggtatcctcg
    27181 gtgcggtgat cgccggtatt atcgtctgga tgctgcatga gcgattccat aatatccgcc
    27241 tgccggatgc gcttgcattc ttcggcggta cgcgcttcgt accaatcatc tcctcgctgg
    27301 tgatgggcct tgtcggcctg gtgattccat tagtctggcc gattttcgcg atgggtatta
    27361 gcggcttggg ccatatgata aacagcgcgg gtgatttcgg accgatgctg tttggtaccg
    27421 gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta
    27481 ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ttgaccatct
    27541 tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc
    27601 tttcgcaagg taaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg ttagctatgt
    27661 atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt tctggcctga
    27721 tcgcctgcgt cgttggcggc actaccgaac cgctggaatt cctgttcctg ttcgtagcgc
    27781 cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc
    27841 tcggcgtcac catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt
    27901 tgcatggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg
    27961 tttactacgt catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg
    28021 atagcgaagt tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt
    28081 acaacgttcc tgcaatcctc gaagcattag gcggtgccga caatattgtc agcctcgata
    28141 actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgctggcac
    28201 tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta
    28261 tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccagg
    28321 cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgta
    28381 cacagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt
    28441 cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc
    28501 acggcgtatt tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact
    28561 ggttttccac ccagcattac accgccatcg attctcagac ggtggtgtat ggcccttctg
    28621 tcatctatat ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa ggcgtggtga
    28681 tccacacacc cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa
    28741 tgcccgttgc tttagagaag caggctgatg gttggttttg cgatatgggc aagttggaag
    28801 ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg
    28861 ggaaagtgtg gacgtgcgat gagctggaga tcatggctga cctatgcgag cgtcatggtg
    28921 tgcgggttat ttccgatgaa atccatatgg atatggtctg gggcgagcag ccgcatattc
    28981 cctggagtaa tgtggcgcgg ggagactggg cgttgctgac gtcgggctcg aaaagtttca
    29041 atattcccgc gctgaccggt gcttacggga ttatagaaaa tagcagcagc cgtgatgcct
    29101 atttatcggc actgaaagga cgtgatgggc tttcttcccc ttcggtactg gcgttaactg
    29161 cccatatcgc cgcctatcag caaggtgcac cgtggctgga tgccttacgc gtctatctga
    29221 aagataacct gacgtatatc tcagataaaa tgaacgccgc gtttcctgaa ctcaactggc
    29281 agatcccgca atccacttat ctggcctggc ttgatctacg tctgttgaat attgacgaca
    29341 acgcgttgca aaaagcgctt atcgagcaag aaaaagtcgc gatcatgccg gggtatacct
    29401 atggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt tcgaaactgg
    29461 aaaaaggtgt ggctggatta ataaacgcca tccgcgctgt tcgctaaccc caattgcgca
    29521 acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg
    29581 cacttttata ttcagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat
    29641 atccatcgcc accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag
    29701 tataatatct cgcttcctgc acaatccctg gaaacactga ttccccacgt tcaggtcatt
    29761 gccaacgaac ccgatctggt gagctttctg accaaacttg actggggcgt taaagttctc
    29821 gcctctcttg atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtcac
    29881 ggcctgcact atgtcgagct gcgtttttca ccaggctaca tggcaatggc acatcagctg
    29941 cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg ccgcaccttt
    30001 ggtgtgcagg cgaagcttat cggcattatg agccggacct tcggcgaagc cgcctgtcag
    30061 caagagctgg aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt
    30121 gatgaacttg gtttcccggg aagtctgttc ctttctcact tcaaccgcgc gcgtgatgcg
    30181 ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat ctggcaggcg
    30241 attcgtgaac tgggtgcgga gcgtattgga catggcgtaa aagccattga agatcgggcg
    30301 ctgatggatt ttctcgccga gcaacaaatt ggtattgaat cctgtctgac ctccaatatt
    30361 cagaccagca ccgtagcaga gctggctgca catccgctga aaacgttcct tgagcatggc
    30421 attcgtgcca gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa
    30481 tataccgttg ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca ggcacagatt
    30541 aatggtctgg aaatggcttt cctcagcgct gaggaaaaac gcgcactgcg agaaaaagtc
    30601 gccgcgaagt aacaaaatgg atggtgcaaa ttgcaccatc catttttcat gcaaggcaca
    30661 aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat caactccatt acctggattg
    30721 cctggcttgc cggaaccgga ttttcaccat cgccatttaa cgcatcacga atagccgcat
    30781 agtaagccgg ataattccca ggtaccgtca acagcgtttc ttcgacacgt tcctcacctt
    30841 ccacgcgggt aagtacgcca tcacgcatat cgtagcccca gtcttcctgc ggcagacgct
    30901 cgccattttt cagacgttct tcctgtggat cgaggccata tttcacataa ctgcctcggg
    30961 atccatgcac gatataccgt gctgactcag cggctgccag catggtaccg tgtaaaatga
    31021 ctcgccgctg tggataggac aagatggcgt ggaaataatc ggtcgactgc gctccgggcc
    31081 gtaactgtgc caaatctacc gtcatgctga ccggtaaacc aaaaagcgta atggcctgat
    31141 caagaagatg tggtgctaaa tcgtaccaga taccgctgcc tgggccgccc tgttcacgcc
    31201 aacgatcgcg cacctgcgga cggaagcggt caaaatgaga ctcaaagtaa gcaacttcac
    31261 ccagcacgcc ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc cagcgacggt
    31321 tatggaatac agacagcaca cgccccaggc tttttgccag cgcatccagc tctcgcgctt
    31381 gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg tttacccgcc tcaagcgccg
    31441 ctttggctaa cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg tctatgttgg
    31501 gatcgttaaa cagatgcttc ggctcagaga caaccgtaac cgttggccag tcggctttta
    31561 cttttgtttc atcactgctg gagattaccg ccagttccag cccgggcgtg cccgcaatca
    31621 ggggcgcatg gaaggttttg ctcgcataac catacccaat caacccaaca cggatgttgt
    31681 cgctcatgtc attgcctctc attgaagtat gatggctatt tgacactatc ctttacccac
    31741 gctcaacagt ttaataacct gccagcaata agggatgttg tttaacttaa gtcaaaaaaa
    31801 tagcgaattt tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa aatcttgctg
    31861 gagctattat tgctaagtaa catttacccc ctgaagttaa tggatcaatc aagagagatg
    31921 tgggctgtaa tgaatcgtct tattgaatta acaggttgga tcgttcttgt cgtttcagtc
    31981 attcttcttg gcgtggcgag tcacattgac aactatcagc cacctgaaca gagtgcttcg
    32041 gtacaacaca agtaagctct gcacttgtgg agcgacatgc tgcccgtccg ggtgcatgtt
    32101 ttcacttgtc ggatattaaa ccaggaattt attatcttgt tcgatgttgt tggtgattgt
    32161 cagggatagt aaagcgcgtt actcttttca gagcagtttt tcatccctga atatttgcca
    32221 tcagcgcatt cttattattg taataacaca agcgttcact tttggtgaaa ttttatcatc
    32281 actaataata cctacccctt tggtattaat tattttatgg accttttatg actgttcagg
    32341 actacttatt aaaatttcgc aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc
    32401 atcttaatta caccctgacg gacgatcagg aactgatcaa tatgtatcgt gctgccgatc
    32461 accgtcgcgc agagctggtt tctggcgggc gtttgtttga cctcggccag gtaccgaagt
    32521 ccgtctggca ctatgtccaa taaagaaagt agcgatcggt tataaaagcc ttataataac
    32581 gcccctaaaa ttagatttgc ctgtttgcgt cgctattctg gagaacctat gaccaccaca
    32641 acgccacaac gtattggagg ctggttgctt ggcccgttgg cctggctttt agtcgctctg
    32701 ttgagtacga cgctggcgtt gttgctgtat accgctgcgt tatcttctcc gcaaacattt
    32761 caaacgctcg gcggacaagc actgacgaca caaatcttat ggggcgtttc ttttattacc
    32821 gctatcgcca tgtggtatta cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt
    32881 tgtgttccta aacactatat tatctggctg cttatttccg tattgctggc agtaaaagca
    32941 ttcgcttttt cacccgtgga agatggcata gccgttcgcc agttgctgtt tactttgttg
    33001 gcaaccgcat tgatcgtccc ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg
    33061 aatccgtaat aaccttacag ttaacctgtt gtcgcctgct ctggattaac ggataatagg
    33121 cggctttttt atttcaggcc gaaaaatgac tgactacctg ttactgtttg tcggaactgt
    33181 actggtcaat aactttgtac tggtcaagtt tctcggtctc tgtccgttta tgggggtttc
    33241 caaaaaactg gaaaccgcga tgggcatggg gctggcaaca acgtttgtga tgacgctggc
    33301 gtctatttgc gcctggctta tcgatacgtg gattttgatc ccacttaatc tgatttacct
    33361 gcgcaccctg gcatttattc tggtgattgc tgtggtcgtg cagttcaccg agatggtggt
    33421 gcgcaaaacc agcccggtgc tttaccgtct gctggggatt tttttgccgc ttatcaccac
    33481 caactgtgca gtgctcggcg tggcgttgct gaatatcaat ctcgggcaca atttcttgca
    33541 gtcggcgctg tacggttttt ccgccgctat cggtttctcg ctggtgatgg tgctcttcgc
    33601 cgccatccgc gaacgccttg ctgtggctga tgtcccggca ccttttcgcg gtcatgccat
    33661 tgcgttaatt accgcaggtc ttatgtctct ggcctttatg ggctttagtg gtttggtgaa
    33721 gttgtaatga atgctatctg gattgccgtt gccgccgtga gcctgctggg cctggcgttt
    33781 ggcgccattc tgggttatgc ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag
    33841 aaaattgacg aaatcttacc gcagagccag tgtggtcagt gcggttatcc cggctgtcgc
    33901 ccctacgcgg aagccatcag ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc
    33961 gaagctgtga tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc gctggatggc
    34021 gaagcgcaag agataacgcc tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt
    34081 ggctgcacta aatgtattca ggcgtgtccg gtagacgcca tcgttggcgc tacccgtgcc
    34141 atgcatacgg taatgagtga tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg
    34201 acgcactgca tctcgttgca accggtcgca gaaacacctg actcctggaa atgggatctg
    34261 aacaccattc ccgtgcgtat cattcccgtg gaacaccatg cttaagttat tctctgcatt
    34321 cagaaaaaat aaaatctggg atttcaacgg cggcatccat ccaccggaga tgaaaaccca
    34381 gtccaacggt acacccctgc gccaggtacc actggcgcag cgttttgtta ttccactgaa
    34441 acagcatatt ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg
    34501 ccagccgctt acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac
    34561 cgttacggct attgcgcccc actctacggc tcatccttca gctttagctg aattaagcgt
    34621 gattattgat gccgatggtg aagactgctg gatcccgcgc gacggctggg ccgattatcg
    34681 cactcgcagt cgcgaagagt taatcgagcg catacatcag tttggtgttg ccgggctggg
    34741 cggtgcagga ttcccgacag gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt
    34801 gattatcaac gcggctgagt gcgagccgta cattaccgcc gatgaccgtt tgatgcagga
    34861 ttgcgcggct caggtcgtag agggtattcg cattcttgcg catattctgc agccacgcga
    34921 aattcttatc ggcattgaag ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct
    34981 ggcggactct aacgatattt ctctgcgggt gattccaacc aaatatcctt ctggcggtgc
    35041 taaacaatta acctacattc tgaccgggaa gcaggttcca catggcgggc gttcatccga
    35101 tatcggcgta ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg ccgttattga
    35161 tggcgagccg attaccgagc gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg
    35221 caacgtctgg gcacggctgg ggacgccagt gcgtcattta ttgaatgatg ccggattctg
    35281 cccctctgcc gatcaaatgg tgattatggg tggcccgcta atgggcttta ccttgccatg
    35341 gctggatgtc ccggtcgtaa agattaccaa ctgtctgttg gctccctctg ccaatgaact
    35401 tggcgaacca caggaagaac aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc
    35461 tgcggatctt ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac acgataaagc
    35521 taccacgcat aacattgctg attgcattga atgtggggct tgcgcgtggg tttgcccgag
    35581 caatattccc ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg ctattcgtca
    35641 ggaagaaaag cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga
    35701 gcgcgaaaaa gcggctcgcc ttgaacgaca taagagcgca gccgttcaac ctgcagccaa
    35761 agataaagat gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg cccaggctac
    35821 acagcctatt gtgattaaag cgggcgaacg cccggataac agtgcaatta ttgcagcacg
    35881 ggaagcccgt aaagcgcaag ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc
    35941 agcaaccgtt gctgatccac gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc
    36001 gcgcaagctg gaac
//
LOCUS       DAZKGS010000028        35773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKGS010000028 DAZKGS010000000
VERSION     DAZKGS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..93)
                     /locus_tag="ACHOQY_001431"
     tRNA            complement(18..93)
                     /locus_tag="ACHOQY_001431"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:His,seq:gtg)
     gene            complement(152..228)
                     /locus_tag="ACHOQY_001432"
     tRNA            complement(152..228)
                     /locus_tag="ACHOQY_001432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(192..194),aa:Arg,seq:ccg)
     gene            complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHOQY_001433"
     CDS             complement(331..1716)
                     /gene="yifK"
                     /locus_tag="ACHOQY_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9677564.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHOQY_001434"
     CDS             complement(1907..2647)
                     /gene="wecG"
                     /locus_tag="ACHOQY_001434"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9677565.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHOQY_001435"
     CDS             complement(2650..4002)
                     /gene="wzyE"
                     /locus_tag="ACHOQY_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9677566.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHOQY_001436"
                     /gene_synonym="wecF"
     CDS             complement(3999..5078)
                     /gene="rffT"
                     /locus_tag="ACHOQY_001436"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9677567.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHOQY_001437"
     CDS             complement(5075..6325)
                     /gene="wzxE"
                     /locus_tag="ACHOQY_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9677568.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHOQY_001438"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6327..7457)
                     /gene="rffA"
                     /locus_tag="ACHOQY_001438"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9677569.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHOQY_001439"
                     /gene_synonym="wecD"
     CDS             complement(7462..8136)
                     /gene="rffC"
                     /locus_tag="ACHOQY_001439"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9677570.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHOQY_001440"
     CDS             complement(8114..8995)
                     /gene="rfbA"
                     /locus_tag="ACHOQY_001440"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9677571.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHOQY_001441"
     CDS             complement(9014..10081)
                     /gene="rffG"
                     /locus_tag="ACHOQY_001441"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9677572.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHOQY_001442"
     CDS             complement(10081..11343)
                     /gene="wecC"
                     /locus_tag="ACHOQY_001442"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9677573.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHOQY_001443"
     CDS             complement(11340..12470)
                     /gene="wecB"
                     /locus_tag="ACHOQY_001443"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9677574.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHOQY_001444"
     CDS             complement(12526..13572)
                     /gene="wzzE"
                     /locus_tag="ACHOQY_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9677575.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHOQY_001445"
     CDS             complement(13584..14687)
                     /gene="wecA"
                     /locus_tag="ACHOQY_001445"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9677576.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHOQY_001446"
     CDS             complement(14927..16186)
                     /gene="rho"
                     /locus_tag="ACHOQY_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9677577.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHOQY_001447"
     CDS             complement(16271..16372)
                     /gene="rhoL"
                     /locus_tag="ACHOQY_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9677578.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHOQY_001448"
     CDS             complement(16513..16842)
                     /gene="trxA"
                     /locus_tag="ACHOQY_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9677579.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHOQY_001449"
     CDS             16973..18238
                     /gene="rhlB"
                     /locus_tag="ACHOQY_001449"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9677580.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18372..19856
                     /gene="gppA"
                     /locus_tag="ACHOQY_001450"
     CDS             18372..19856
                     /gene="gppA"
                     /locus_tag="ACHOQY_001450"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9677581.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHOQY_001451"
     CDS             complement(19903..21924)
                     /gene="rep"
                     /locus_tag="ACHOQY_001451"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9677582.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22141..22590
                     /locus_tag="ACHOQY_001452"
     CDS             22141..22590
                     /locus_tag="ACHOQY_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9677583.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHOQY_001453"
     CDS             22789..23070
                     /gene="ppiC"
                     /locus_tag="ACHOQY_001453"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9677584.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHOQY_001454"
     CDS             complement(23157..24632)
                     /gene="ilvC"
                     /locus_tag="ACHOQY_001454"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9677585.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            24782..25675
                     /gene="ilvY"
                     /locus_tag="ACHOQY_001455"
     CDS             24782..25675
                     /gene="ilvY"
                     /locus_tag="ACHOQY_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9677586.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(25727..27271)
                     /gene="ilvA"
                     /locus_tag="ACHOQY_001456"
     CDS             complement(25727..27271)
                     /gene="ilvA"
                     /locus_tag="ACHOQY_001456"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9677587.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27274..29124)
                     /gene="ilvD"
                     /locus_tag="ACHOQY_001457"
     CDS             complement(27274..29124)
                     /gene="ilvD"
                     /locus_tag="ACHOQY_001457"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9677588.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(29189..30118)
                     /gene="ilvE"
                     /locus_tag="ACHOQY_001458"
     CDS             complement(29189..30118)
                     /gene="ilvE"
                     /locus_tag="ACHOQY_001458"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9677589.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30138..30401)
                     /gene="ilvM"
                     /locus_tag="ACHOQY_001459"
     CDS             complement(30138..30401)
                     /gene="ilvM"
                     /locus_tag="ACHOQY_001459"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9677590.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30398..32044)
                     /gene="ilvG"
                     /locus_tag="ACHOQY_001460"
     CDS             complement(30398..32044)
                     /gene="ilvG"
                     /locus_tag="ACHOQY_001460"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9677591.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32047..32097)
                     /gene="ilvX"
                     /locus_tag="ACHOQY_001461"
     CDS             complement(32047..32097)
                     /gene="ilvX"
                     /locus_tag="ACHOQY_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9677592.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32184..32282)
                     /gene="ilvL"
                     /locus_tag="ACHOQY_001462"
     CDS             complement(32184..32282)
                     /gene="ilvL"
                     /locus_tag="ACHOQY_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9677593.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
     gene            32635..34155
                     /gene="yifB"
                     /locus_tag="ACHOQY_001463"
     CDS             32635..34155
                     /gene="yifB"
                     /locus_tag="ACHOQY_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9677594.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(34180..34518)
                     /gene="maoP"
                     /locus_tag="ACHOQY_001464"
     CDS             complement(34180..34518)
                     /gene="maoP"
                     /locus_tag="ACHOQY_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9677595.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            34637..35476
                     /gene="hdfR"
                     /locus_tag="ACHOQY_001465"
     CDS             34637..35476
                     /gene="hdfR"
                     /locus_tag="ACHOQY_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9677596.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(35572..35647)
                     /locus_tag="ACHOQY_001466"
     tRNA            complement(35572..35647)
                     /locus_tag="ACHOQY_001466"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35612..35614),aa:Trp,seq:cca)
     gene            complement(35656..35732)
                     /locus_tag="ACHOQY_001467"
     tRNA            complement(35656..35732)
                     /locus_tag="ACHOQY_001467"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35696..35698),aa:Asp,seq:gtc)
BASE COUNT         8733 a         9751 c         9034 g         8255 t
ORIGIN      
        1 gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac
       61 aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta
      121 ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt cgaacctgag
      181 acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg
      241 cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgcg
      301 cgtttggtta cggtttgtgc gttttgctga ttattcctcc agtttatgcg ctttcccgtg
      361 gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccaac
      421 aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa
      481 tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat
      541 cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac
      601 catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat
      661 gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac
      721 tggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt ttttcgccag
      781 tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt
      841 cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag
      901 tacgaacggg ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa
      961 aatcgcgcct acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag
     1021 cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta
     1081 ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg caaagaaacc
     1141 gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa
     1201 aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa
     1261 ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc
     1321 cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact ggacataaac
     1381 gccaatggcg gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc
     1441 ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga acgaaccggt
     1501 aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga cgaatagccc
     1561 ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac tggcggcccc
     1621 cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc
     1681 cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg
     1741 ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat
     1801 tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag
     1861 acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg
     1921 tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg
     1981 aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta
     2041 cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca
     2101 ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga
     2161 tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat
     2221 aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta gcttcagttt
     2281 gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc
     2341 gcgccatcag ctcttcccag agatcagcac cggcaacgcg ggaaacctgc gcctgcgggt
     2401 actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat tcggcagcgt
     2461 taattagctc cctgacctca gcattatctt caatggtcag cattttttcg gcattaatgg
     2521 caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg gcgtgctgca
     2581 tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc atggtgttgt
     2641 tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag
     2701 tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac
     2761 cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat
     2821 catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagg
     2881 ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac caaccacgat
     2941 cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg
     3001 cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt tggctgagtt
     3061 cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat agaaatcgcg
     3121 gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag
     3181 attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc
     3241 gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca caatccccag
     3301 cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag
     3361 gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag
     3421 caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat cctggcgcag
     3481 aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc
     3541 ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt
     3601 gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca cccacgtaag
     3661 attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt
     3721 gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa
     3781 cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct
     3841 ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa
     3901 gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag
     3961 cagccagacc acaaacaggc cactgaattg cagcagactc atgcgccctc cccggcggca
     4021 atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt
     4081 ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg
     4141 gtagtaaaca gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttcccgg
     4201 ttaagcacac aaggaatgcc cgcctgaatc agtaagcaca gcgtaccaat gccctgctgg
     4261 cgagcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat
     4321 tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga
     4381 cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt
     4441 accgtatcgc caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgagg
     4501 tcgccggagt tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc
     4561 gtattgagag aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg
     4621 tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt
     4681 tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac
     4741 aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga
     4801 gccagccaca gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct
     4861 tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa
     4921 agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg
     4981 tgctcgctcg tcgcagccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga
     5041 gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa
     5101 acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc
     5161 ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc
     5221 tgatttccgc cagaatataa aaccgcaatg acgctttggc gatcaccaga taaccaaaga
     5281 cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag
     5341 cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc cagacggtaa
     5401 aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga
     5461 tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg
     5521 taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat
     5581 cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca
     5641 ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc
     5701 agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg atagtggcag
     5761 gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca
     5821 aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg
     5881 cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga
     5941 tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca
     6001 atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac
     6061 caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt
     6121 actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc acgccgagca
     6181 cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacacag
     6241 ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc
     6301 acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc
     6361 gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc
     6421 tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa
     6481 ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag aaagttaatc
     6541 aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg
     6601 cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgca
     6661 tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt
     6721 tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc
     6781 caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg
     6841 atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca
     6901 cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc
     6961 agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac cacaaacaga
     7021 ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg
     7081 accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg
     7141 ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag cacaaaggca
     7201 ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg
     7261 agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg
     7321 ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac
     7381 agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt
     7441 gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc
     7501 accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa
     7561 agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctac
     7621 accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac
     7681 ataaccgcga ataccgccgg acgccgcacg taaaatcaga cattgatgat caaaggtgcc
     7741 gcgcacggca ttttcaatcc actgtgcata aaagcgtctg ctggcgtcag gcgcatacca
     7801 cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta atgcgggaat
     7861 atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg gtagtgccaa
     7921 atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca gttcacccgt
     7981 atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt ctggcgtcag
     8041 gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc
     8101 ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc
     8161 cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc
     8221 cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg
     8281 tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa
     8341 atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg
     8401 aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac tccacgactt
     8461 tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt
     8521 cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag cgttccgggt
     8581 ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct
     8641 tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac
     8701 cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt
     8761 cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac
     8821 ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac atcagcaccg
     8881 acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc
     8941 gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct ttcatgtttg
     9001 cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca
     9061 cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc attccacttt
     9121 caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca tcaatggcat
     9181 aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga tagtgcgcca
     9241 cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt tccacaacat
     9301 cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg
     9361 tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct
     9421 gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga
     9481 tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg
     9541 gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctactggct tttgacgcgg
     9601 aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca
     9661 ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt
     9721 ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt
     9781 tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca
     9841 gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc
     9901 agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc agcgacatca
     9961 ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga
    10021 tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca
    10081 tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt cgccattgat
    10141 aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc
    10201 gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg
    10261 ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc
    10321 cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt
    10381 cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg
    10441 atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc
    10501 ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac
    10561 gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc
    10621 ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt
    10681 cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac
    10741 gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg
    10801 cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa
    10861 cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa
    10921 agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg
    10981 cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc
    11041 agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg tgggtacagc
    11101 aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac
    11161 ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat
    11221 ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa
    11281 cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact
    11341 catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca
    11401 ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt taaaagacgc
    11461 gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc
    11521 accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc
    11581 gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt catcagccag
    11641 acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg
    11701 cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc
    11761 tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa
    11821 ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat aaacgggtaa
    11881 tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg cacccataac
    11941 agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac gttttcacgc
    12001 agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc
    12061 aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg taaaccagcc
    12121 tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtt
    12181 gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc
    12241 ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga
    12301 tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc ccgatgctgc
    12361 gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga
    12421 gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc
    12481 ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt tcgagcaacg
    12541 gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag
    12601 gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata
    12661 ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca tggcccgatt
    12721 ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg
    12781 gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc
    12841 tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatgctgtt
    12901 catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat
    12961 ctggatggtg cgtgccgccc atgcgccttt cagctcctca ttcagatggc tggctgcacg
    13021 ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc
    13081 aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa actggatgtt
    13141 gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgctt
    13201 gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac
    13261 aaactcttta taggcttcgt ccatgaccga tggttggtcg gcagaagcca tgtttgaacg
    13321 gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt
    13381 tggacgatcg gtaatcgccg tcgcgctcca ctcttgtcga gcaaaaaaag tatacgccag
    13441 cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt
    13501 acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat
    13561 tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta
    13621 cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa
    13681 tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa
    13741 tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc
    13801 acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg
    13861 tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc
    13921 attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc
    13981 tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca
    14041 cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg
    14101 atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa
    14161 atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca
    14221 tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca
    14281 aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt
    14341 ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg
    14401 ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga
    14461 tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg
    14521 taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt
    14581 ttatccacta aaccgacttt ttttgccact ttacgggcaa aaaacagaaa cagtgtcgtg
    14641 aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc
    14701 agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt
    14761 tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg cataacatgc
    14821 cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta cagatataaa
    14881 agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca
    14941 tcatttcgaa gaaatcgtca ttggtcttag tcatggccag tttattaatg aggaattcca
    15001 ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt
    15061 cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg
    15121 ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt
    15181 taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga
    15241 taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag cgtttcggac
    15301 gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag
    15361 tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt
    15421 gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg
    15481 cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta
    15541 cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag
    15601 caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa
    15661 tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga gcggttaaat
    15721 cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta
    15781 agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa
    15841 ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg
    15901 tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag
    15961 gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct
    16021 ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa
    16081 aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt tcgccgagag
    16141 tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc
    16201 ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc
    16261 ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac
    16321 tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata
    16381 aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt
    16441 caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa
    16501 catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag
    16561 tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc
    16621 acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt
    16681 cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg
    16741 accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt
    16801 gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat
    16861 aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat
    16921 ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa
    16981 aacacattta acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc
    17041 ccttgaaaaa aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac
    17101 gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt
    17161 tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa
    17221 tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga
    17281 cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg gtggtgatgg
    17341 ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg gcactacggg
    17401 gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc aggtggtggt
    17461 actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt
    17521 ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta
    17581 ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc
    17641 ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa
    17701 aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc
    17761 caataccaaa taccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt
    17821 cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac
    17881 ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc
    17941 ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg
    18001 tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga
    18061 gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa
    18121 atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg
    18181 tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat
    18241 aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat
    18301 gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa
    18361 ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata
    18421 gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa
    18481 aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa
    18541 tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct
    18601 cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta
    18661 ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag
    18721 cacgtctcat ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg
    18781 tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt
    18841 tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc
    18901 tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg
    18961 ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg
    19021 cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc
    19081 agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc
    19141 tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta
    19201 ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct
    19261 acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc
    19321 agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact
    19381 tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca
    19441 gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg
    19501 ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc
    19561 tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa
    19621 atgccgtacc tccgcgcgtc gcagaacaac tctgccgttt actccgcctg gccatcattt
    19681 ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac
    19741 tgttgacctt gacgcttccg caaggttggc taactcaaca tccgctgggt aaagagatta
    19801 ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag
    19861 taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc
    19921 cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc
    19981 gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa
    20041 gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac acagcgtaaa
    20101 ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat
    20161 attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta
    20221 gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca cctgatccag
    20281 ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg
    20341 ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa
    20401 cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta
    20461 cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg
    20521 ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc
    20581 acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt
    20641 gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg
    20701 cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata
    20761 agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta
    20821 cgggatgcgg ttttgcatca ggaatttttc aaacactcgc gactgatggt taccgcgata
    20881 aagaatagcg taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc
    20941 agtgacgcgc tcggcttcat gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc
    21001 gtaacccagt tcggagaaca gacgcttctc aaagacgtgc gggttattgg cgatcaggat
    21061 gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt
    21121 cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga
    21181 gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag
    21241 tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg
    21301 aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa
    21361 taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat catacagccc
    21421 ataacaatgg gcaaaaatac gatcccgctc gccctttgcc tctgccgccg cctgcgccgg
    21481 agttttgaga tcgttcttcc agttggagat ggtcgaaatc agctgttgca ggagaacttt
    21541 gtcatcttca atcagcccct cggtcaactc tttaagcaaa gcaagctgat cggtatcgtc
    21601 aaacagcgaa aagttcgcct tcatcccaag cgccgcatac tcgcgtttga tgatatccaa
    21661 ccccagcgtg tgaaaagtgg agatcatcaa cccgcgcgcc tctttgcgac ccagcgtctg
    21721 cccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg
    21781 tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt
    21841 tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg
    21901 ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt
    21961 gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg gctgcggatg
    22021 tttaaatcag agagagaaga ccgaaatcgg agttcatcgt gaatccatta gacttagaaa
    22081 atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag aggtttactc
    22141 gtggcgatta atttcagccc taaggttggt gaaatacagg aatgcaactt tggtaactat
    22201 cctgttatcc agaatggaca gttcagcacc acgtattacg atggccgcat tcctcctgaa
    22261 atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa cgcatgtatc
    22321 gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat gcatgttgaa
    22381 atagccagta atgttatcaa tggcctgcaa ttctttgatc agcagattcg ttgggccaaa
    22441 gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac ttacagaggg
    22501 tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc ggtcatcaag
    22561 tcaatcaatg ccatatcgct cataaattga caataaaata accatccccg ataatgactt
    22621 tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc ccggattttc
    22681 cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt agcatctgcg
    22741 gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat ggcaaaaaca
    22801 gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgagcag
    22861 attaagaacg gtgccgattt cggcaagctg gcgaagaaac actctatttg cccgtcaggc
    22921 aaacgcggcg gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa
    22981 gtggttttct cttgtccggt actggagccg accggcccgc tgcacaccca gttcggatat
    23041 cacatcatta aggtgctgta ccgcaactaa tagcaaggcc ttctccagga gaaggccttg
    23101 agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc gcgcacttaa
    23161 cccgcaacag caatacgttt catatctgtc atatagccgc gcagtttctt acctacctgc
    23221 tcaatcgcat ggctgcgaat cgcttcgttc acatcacgca gttgcgcgtt atctaccgcg
    23281 ccttccggaa tagctttacc caagtcgccc ggttgcagct ctgccataaa cggtttcagc
    23341 aacggcacac aagcgtaaga gaacagatag ttaccgtact cagcggtatc agagataacc
    23401 acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg cagctcgtgc
    23461 agtgattcat aatatgcaga ctcttcaatg atgccggaat cgaccatggt ttcgaacgcc
    23521 agttcaacgc ccgcttttac catcgcgatc atcagtacgc ctttatcgaa gtactcctgc
    23581 tcgccgattt tgccttcata ctgcggcgcg gtttcaaacg cggttttgcc ggtctcttca
    23641 cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc ggaagagaat
    23701 tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat ctctttcagc
    23761 tgttctgaca gcgcataagc acgcagtttc gccgggttag agagacggtc catcatcagg
    23821 gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg aattagtttt
    23881 tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag cagcgagcca
    23941 gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt cacttccgca
    24001 acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc ccatgcttta
    24061 gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat cagcgtcggt
    24121 acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt cggcgcaacc
    24181 atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt gaaaccgtgc
    24241 gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac tacatcagag
    24301 tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag ttcttcgtaa
    24361 gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt ctcggcaatc
    24421 gcttctttac gcagagcgta ggagatatcc agaccagaat cacgcatgtt caggccctgg
    24481 ttcagaccct gtgcgccaca gccgacgatg actacttttt taccctgaag gtagctcgcg
    24541 ccatcggcga attcatcgcg ccccataaag cgacatttgc ccagctgtgc cagctgctga
    24601 cgcagattca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt
    24661 attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt tattgcggaa
    24721 attgatatat tcacaacgtc acattgcaat ttttgcaacg tcaacatcga gggctgcgcc
    24781 attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc attttggccg
    24841 cagcgcgcgg gcgatgcacg ttagcccatc cacgctctca cggcagattc agcgcctgga
    24901 agaagatctt ggtcagccgc tatttgtgcg cgataaccgc acggtaacgc tgacggaagc
    24961 gggcgaagag ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc aattgcgcca
    25021 caccatcgat cagcaagggc cgtcgctctc tggcgaatta catatcttct gctcggtgac
    25081 cgctgcctac agccatctgc cgccgattct ggatcgcttc cgcgcggaac acccgtcggt
    25141 ggagatcaaa ctcaccactg gtgatgcggc agatgcaatg gaaaaagtgg tcacgggaga
    25201 agcggatctg gcgattgccg ggaaaccgga gacgctaccc ggcgctgtgg cgttttcgat
    25261 gctggagaat ctggcagtag tgctgattgc gcccgcgctg ccctgcccgg tgcgtaacca
    25321 ggtttcggca gagaagccgg actggtctac agtgccgttt atcatggccg atcagggacc
    25381 ggtgcgtcgc cgcattgaac tgtggtttcg acgcaataaa atcagcaacc cgatgattta
    25441 cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct gtggtgtggc
    25501 gctgttgccg gaagtagtgc tggaaaacag ccccgaaccg gtgcgtaacc gcgtgatgat
    25561 tttagagcgc agcgatgaaa aaacgccgtt tgaactgggc gtgtgtgcac aaaaaaagcg
    25621 gctgcatgag ccgctgattg aggcgttctg gaggattttg ccgaaccaca aatgacgttg
    25681 tcgcgcgggt aggcctgata agcgaagcgc tatcaggcat ttttccctaa cccgccaaaa
    25741 agaacctgaa cgccgggtta ttggtttcgt cgtggcaatc gtagcccagc tcattcagcc
    25801 gggtttcgaa atccggttca tggtcgccaa gttcgaacgc cgccagtacg cgcccgtagt
    25861 cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga
    25921 ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg cgttcctgca
    25981 acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc atttcgtcgt
    26041 cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct
    26101 cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg gcatcggcaa
    26161 aacggtagtt gaactcggtg accgatcgcc cgccaagcag ttggcagaat ttgaggaagc
    26221 tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg cccagttcgc
    26281 agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa agaatatgcg
    26341 ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc gccagcgcca
    26401 gcgcgccaga gggttccgcc accgcgcgca catcttcgaa taaatccttc atcgccgcac
    26461 agatcgcatc actatcgacg gtgatgatgt cgtcgagata ctcctggcat aaacggaagg
    26521 tttcgtcacc gatgcgtttt accgctacgc cttcagcaaa tagccctacg cgcggcagat
    26581 caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg
    26641 cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgcaacgccc gccgccagac
    26701 cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg agcagttcca
    26761 gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc acccaggtga
    26821 acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat
    26881 gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg
    26941 ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg acgccctgcg
    27001 cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg
    27061 ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga tcttcgcgct
    27121 tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt agcggcgtaa
    27181 cctgcgccgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg gcaccttccg
    27241 gagcaccgga caggggttgc gagtcagcca ttattaaccc cccagtttcg atttatcgcg
    27301 caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg caaaggaaac
    27361 ctgacgttca cggtttttcg gcgtccaggc tttgtcacct cgagcgtcct gcgcttcacg
    27421 acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg ggatgtctat
    27481 agcgatcagg tcaccatctt caatcaggcc aatgctgccg ccgcttgccg cttccggtga
    27541 gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc
    27601 acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca tttcctgcat
    27661 gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg cgacaacttt
    27721 gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacactttcg ccgggccggt
    27781 gaatttgagg atgctgtcat cgacgcccgc ggttttaacg atgcagccgt tttctgcgaa
    27841 attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat
    27901 acagccattg gcgcgatcgt catcgagaga atcccaacgg caatcctgcg agaatgcctg
    27961 tgtggtacga atgcctgcag gacctgcgcg gaacatattt tttaccgcgt catcctgggt
    28021 cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccaagta cgtttttcac
    28081 atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa caccaccagc
    28141 acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac acagctgtgg
    28201 aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg cttcctgcgc
    28261 cgccgccagc aggtgaagta cggtgttagt cgatccaccc atcgcgatat ccagcgtcat
    28321 ggcgttttca aacgccgctt tgctagcaat attgcgcggc agtgcacttt cgtcgttttg
    28381 ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg gcattgagga acaactgctt
    28441 acggtcggcg tgggttgcca gcagcgagcc gttgcccggc tgcgacagac ccagcgcttc
    28501 ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggagccgc aggtcggaca
    28561 cgcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg cgccctggat
    28621 catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt tcccggcctc
    28681 catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag ccatcagcat
    28741 ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc agtgggcgtt
    28801 gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggcagtgaat aaagcatccc
    28861 cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt tggcaacgcc
    28921 gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc
    28981 cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa aatcggcgtc
    29041 ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac catgagtggt
    29101 ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct cgtaaatggg
    29161 acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac cagccccatt
    29221 tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg
    29281 ggccacaacg gccttcgcca acctgaatac catctacact gcgcactggc gtgatttctg
    29341 ccgcagtacc ggacataaac acttcatccg ccaggtacag ggattcgcgc gacagaacct
    29401 gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca cgggtaatac
    29461 ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac accatctttc acttcaaaca
    29521 ggttttcgcc tgcgccttca gagatataac cgttcacatc cagcgcgata ccttcctgat
    29581 aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gagatagtta ccaccggctt
    29641 ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc atcgcatcga
    29701 tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagcg gcgataatca
    29761 catcggttga gtatcccgct ggcgggttta cacccatgcc aacatcacca acgaagatca
    29821 gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga caagcttcca
    29881 tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag tcatgcagac
    29941 gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga
    30001 tgccttcaaa aaccgaagtg ccatagtgca gcgcgtgcga catcacatgc accttcgcgt
    30061 cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc gtggtcattt
    30121 ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg
    30181 gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc gaccgaccgt
    30241 gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc agccatattc
    30301 attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc taaggtttcc
    30361 ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc tccaacattt
    30421 ctgaattact ggcgccaggc ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga
    30481 catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct tcaacctggt
    30541 ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg
    30601 ggttatcagt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc
    30661 gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac tgcttgcgtt
    30721 ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag atacagacga
    30781 cagtatcgtt cggtcgtgcg acttgtgcgc caaccgccgc tggtaaaccg aaacccatgg
    30841 tgcctaagcc gctggaggta atgaaatttt ccgggcgagt gtgtgcgatg tgctgcgcgg
    30901 cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt ttacgatccg
    30961 acagttgttt taacaacaac ggcgcgtaga tagcgtcacc gggatggtcg taacgccagg
    31021 tatgttcatc acgcagctgc gcgcagtatt gctgccagtc attgatattt aacggctgct
    31081 gtaatgctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct
    31141 tgttcatttc tgccgggtcg atatccatat ggataacact ggcgtgtggt gcgaaggtgt
    31201 tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact
    31261 cctgcaccgc gaagttcgcc gctttggtgc catgcattcc cagcatgccc aggtagtacg
    31321 gataatctgc ttcaactgcg cccagccctt tcagcgtgca ggtggcaggc atttttgtgg
    31381 tagcgagaaa ttctcgtaaa gcaggaactg cctgcgccat acccacgcca ccaccaacgt
    31441 acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcaact tcggcatgtg
    31501 ggaaagtcac ttcgttttca acggtggtga accacggttc caggtcaccg ctggctagct
    31561 ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg gcaacgtcga
    31621 atgcttcagc cataatgcgc ggcaactctt ccagcgactg caccagaaag ctgtgcttgg
    31681 tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcgtccgtg ccgataaacg
    31741 gtgcggacac ttgaccggtg atggcaacaa cagggataga atctaacagt gcgtccgcaa
    31801 gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg ccagttttgc
    31861 cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt cggcacagca
    31921 agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg ccacccggat
    31981 aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac tgtgcgccat
    32041 tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt tattcattgt
    32101 ctgctcctcg gttatatttt taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt
    32161 cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc cccgcacggt
    32221 gggataataa tcaccaccac gctaatcatg accaggctaa tcactcgtag aagggctgtc
    32281 attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca tagattttgc
    32341 gaatagcaca agatattttt tggccgattt ttatgatgcg tttaataatt tcaatagaaa
    32401 ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaaatt caatttcagt atgaatgttg
    32461 ttcccttcct cgtagttcat cctttgtccg ctgcattttt gcgagcatca ttccaccgcc
    32521 agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa ttcacgaatt
    32581 agcgggctac agcagcgata tcttatgcac ataatagtga ctcaaggagg gcttatgtca
    32641 ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg cgcccccaat cactgttgag
    32701 gtacatatca gtaaaggtct acccggctta acgatggtgg gcttaccaga aacaactgta
    32761 aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga atatccggcg
    32821 aaaaaaatca ccatcaacct ggctccagcc gatctgccaa aagaaggggg acgatatgat
    32881 ttacctatcg ccattgcgtt gctggcggcc tcagaacagc ttacagccaa taagttagat
    32941 gaatatgaat tagtcggaga actggcgctt acaggcgctc tgcgtggcgt tcccggcgca
    33001 atctccagtg caactgaagc tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac
    33061 gaagatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca tctgcaggct
    33121 gtctgtgcgt ttctggaagg taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta
    33181 tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggagcaagg aaagcgagga
    33241 ctggaaatta ccgccgctgg cgggcacaac cttttactga ttgggccgcc gggaacaggt
    33301 aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa tgaagaggca
    33361 ctggagagcg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa acaatggcgg
    33421 cagcgcccgt tccgctcacc gcatcacagt gcgtcgttaa ctgcgatggt gggcggtggt
    33481 gcaattccag ggccaggtga aatttcgttg gcgcataacg gcgtgctttt tcttgatgag
    33541 ctacctgaat ttgaacggcg tacactggat gccttgcgag agccgattga atccgggcag
    33601 atccatcttt cacgcacacg agccaaaata acctatccag cccgtttcca gcttgttgcg
    33661 gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg cacgccagaa
    33721 cagacattac gttatctcaa ccggctctcg gggccctttc tcgaccgctt cgatctctca
    33781 ctggagatcc cattaccacc ccccggcatt ttgagtaaaa cggtagtgcc gggagaaagc
    33841 agcgccaccg ttaaacaacg cgtaatggcc gccagagagc gccaatttaa gcggcagaat
    33901 aaactgaatg cctggctgga tagtccggaa atacgccaat tctgcaaact tgagagcgaa
    33961 gatgcgatgt ggttggaagg aacactgatc catctggggt tatcgattcg tgcctggcag
    34021 cggttattga aagttgcacg aaccattgct gatattgatc agtctgacat tatcacacgt
    34081 cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat ccatctgcaa
    34141 aaactactga cataaaaaaa gggcatttcg ccctttttat taatcatcag aatcggtgta
    34201 gtcttcagca ccttcaacct gcggtttacc gccggaaagg gtgtgaaaac gttttggacg
    34261 cttaatacgc gtcatatact tggaccacac gcgttctgct tctgtcactg gctcacgttc
    34321 gccacggcat actgctacga agagtttctc ttcctcggta accggctcgc gtttgccaag
    34381 atccaactca ttgaaggcat aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa
    34441 atcaccatga cgagagaatc cacgtggata atgtttattg tcgaaatatc gattagtcgt
    34501 cgtaaagctt tccgccatcc tgcacgctcc taattctttg accgagctag ttatggcgcg
    34561 gagtattagt tacgcttgac agagtgtaaa acaaaacatt taaatcataa cgacaaataa
    34621 ttttgcggag agcactgtgg atacggaatt gttaaaaact ttcctggaag ttagccgaac
    34681 gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc cagtcagcag tgagctttcg
    34741 aatcagacaa ctggaaaatc aactgggtgt gaaccttttc acccgccaca gaaacaatat
    34801 ccgtttaacc gctgccggtg aaaaactact gccttatgca gaaacgctca tgagcacctg
    34861 gcaggccgcc cgcaaggagg tggcgcatac ctcacgacat aacgagtttt ctatcggtgc
    34921 cagcgcctca ttgtgggaat gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca
    34981 ggatgcccat acaggcttac agttcgaagc gcgaattgcc caacggcagt ctctggtaaa
    35041 acagctgcat gaacgccagc ttgatcttct tattaccact gaagcgccca aaatggacga
    35101 atttagtagt cagttgctgg gatatttcac tttagcgctt tataccagtg ccccttcaaa
    35161 actaaaggga gatcttaatt atctgcgact tgagtggggg ccagattttc aacagcatga
    35221 ggcaggtttg atcggtgctg acgaagtgcc cattctgaca accagttctg ctgaactggc
    35281 acagcaacag attgcgatgc ttaatggttg cacctggcta cccgtcagct gggcgcgtaa
    35341 aaaaggcggc ctgcataccg ttgtcgatag cacaacactt tcacggccgc tttatgccat
    35401 atggctgcaa aatagcgata aaaatacgtt gattcgcgat cttttgaaaa ttaacgtgct
    35461 ggatgaagtg tattaatatg aatggctggc aaggatgccg gtagaaggat ttacttcgga
    35521 gagggttatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggcagggg
    35581 cggagagact cgaactccca acacccggtt ttggagaccg gtgctctacc aattgaacta
    35641 cgcccctaat tagggtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
    35701 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
    35761 atgaccggct tac
//
LOCUS       DAZKGS010000029        35146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKGS010000029 DAZKGS010000000
VERSION     DAZKGS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..218)
                     /locus_tag="ACHOQY_001468"
     CDS             complement(<1..218)
                     /locus_tag="ACHOQY_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677597.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHOQY_001469"
     CDS             complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHOQY_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9677598.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACHOQY_001470"
     CDS             complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACHOQY_001470"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9677599.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACHOQY_001471"
     CDS             complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACHOQY_001471"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9677600.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACHOQY_001472"
     CDS             complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACHOQY_001472"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9677601.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACHOQY_001473"
     CDS             complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACHOQY_001473"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9677602.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACHOQY_001474"
     CDS             complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACHOQY_001474"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9677603.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACHOQY_001475"
     CDS             complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACHOQY_001475"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9677604.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6496..7545)
                     /locus_tag="ACHOQY_001476"
     CDS             complement(6496..7545)
                     /locus_tag="ACHOQY_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9677605.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7677..8894
                     /locus_tag="ACHOQY_001477"
     CDS             7677..8894
                     /locus_tag="ACHOQY_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677606.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACHOQY_001478"
     CDS             complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACHOQY_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9677607.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9528..10193)
                     /locus_tag="ACHOQY_001479"
     CDS             complement(9528..10193)
                     /locus_tag="ACHOQY_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9677608.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10414..10659
                     /gene="tusA"
                     /locus_tag="ACHOQY_001480"
     CDS             10414..10659
                     /gene="tusA"
                     /locus_tag="ACHOQY_001480"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9677609.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHOQY_001481"
     CDS             complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHOQY_001481"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9677610.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13033..13659)
                     /locus_tag="ACHOQY_001482"
     CDS             complement(13033..13659)
                     /locus_tag="ACHOQY_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9677611.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13800..14159
                     /locus_tag="ACHOQY_001483"
     CDS             13800..14159
                     /locus_tag="ACHOQY_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9677612.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14162..14431)
                     /locus_tag="ACHOQY_001484"
     CDS             complement(14162..14431)
                     /locus_tag="ACHOQY_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9677613.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHOQY_001485"
     CDS             complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHOQY_001485"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9677614.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15167..16663
                     /gene="ftsY"
                     /locus_tag="ACHOQY_001486"
     CDS             15167..16663
                     /gene="ftsY"
                     /locus_tag="ACHOQY_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9677615.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16666..17334
                     /gene="ftsE"
                     /locus_tag="ACHOQY_001487"
     CDS             16666..17334
                     /gene="ftsE"
                     /locus_tag="ACHOQY_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9677616.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17327..18385
                     /gene="ftsX"
                     /locus_tag="ACHOQY_001488"
     CDS             17327..18385
                     /gene="ftsX"
                     /locus_tag="ACHOQY_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9677617.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18630..19484
                     /gene="rpoH"
                     /locus_tag="ACHOQY_001489"
     CDS             18630..19484
                     /gene="rpoH"
                     /locus_tag="ACHOQY_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9677618.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19755..20858
                     /gene="livJ"
                     /locus_tag="ACHOQY_001490"
     CDS             19755..20858
                     /gene="livJ"
                     /locus_tag="ACHOQY_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9677619.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21057..21440)
                     /gene="panM"
                     /locus_tag="ACHOQY_001491"
     CDS             complement(21057..21440)
                     /gene="panM"
                     /locus_tag="ACHOQY_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9677620.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            21864..22973
                     /gene="livK"
                     /locus_tag="ACHOQY_001492"
     CDS             21864..22973
                     /gene="livK"
                     /locus_tag="ACHOQY_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9677621.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23021..23947
                     /gene="livH"
                     /locus_tag="ACHOQY_001493"
     CDS             23021..23947
                     /gene="livH"
                     /locus_tag="ACHOQY_001493"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9677622.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23944..25221
                     /gene="livM"
                     /locus_tag="ACHOQY_001494"
     CDS             23944..25221
                     /gene="livM"
                     /locus_tag="ACHOQY_001494"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9677623.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25218..25985
                     /gene="livG"
                     /locus_tag="ACHOQY_001495"
     CDS             25218..25985
                     /gene="livG"
                     /locus_tag="ACHOQY_001495"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9677624.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25987..26700
                     /gene="livF"
                     /locus_tag="ACHOQY_001496"
     CDS             25987..26700
                     /gene="livF"
                     /locus_tag="ACHOQY_001496"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9677625.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27096..28412
                     /gene="ugpB"
                     /locus_tag="ACHOQY_001497"
     CDS             27096..28412
                     /gene="ugpB"
                     /locus_tag="ACHOQY_001497"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9677626.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28510..29397
                     /gene="ugpA"
                     /locus_tag="ACHOQY_001498"
     CDS             28510..29397
                     /gene="ugpA"
                     /locus_tag="ACHOQY_001498"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9677627.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29394..30239
                     /gene="ugpE"
                     /locus_tag="ACHOQY_001499"
     CDS             29394..30239
                     /gene="ugpE"
                     /locus_tag="ACHOQY_001499"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9677628.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30241..31311
                     /gene="ugpC"
                     /locus_tag="ACHOQY_001500"
     CDS             30241..31311
                     /gene="ugpC"
                     /locus_tag="ACHOQY_001500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9677629.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            31308..32051
                     /gene="ugpQ"
                     /locus_tag="ACHOQY_001501"
     CDS             31308..32051
                     /gene="ugpQ"
                     /locus_tag="ACHOQY_001501"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9677630.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32038..32478)
                     /locus_tag="ACHOQY_001502"
     CDS             complement(32038..32478)
                     /locus_tag="ACHOQY_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9677631.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32598..34340
                     /gene="ggt"
                     /locus_tag="ACHOQY_001503"
     CDS             32598..34340
                     /gene="ggt"
                     /locus_tag="ACHOQY_001503"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9677632.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(34378..34662)
                     /locus_tag="ACHOQY_001504"
     CDS             complement(34378..34662)
                     /locus_tag="ACHOQY_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP9677633.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(34862..35017)
                     /gene="yrhD"
                     /locus_tag="ACHOQY_001505"
     CDS             complement(34862..35017)
                     /gene="yrhD"
                     /locus_tag="ACHOQY_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFP9677634.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         8191 a         9097 c         9795 g         8063 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccc
       61 agcagcggat tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatacg taccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcac cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttctattatt
      241 ttaatgacag tatcttcata cgaaaaagcg aagaaaaact tgtggtcaca atgaattgaa
      301 gatttttagg aatatattta gaacgttaca aaagagaagg ttcggatagg gtatttagag
      361 aggaatcagg tgtgctagtg gccctttgtt gatcgttcac caaaggaccc aataatagac
      421 tcaatcttcc ttcggcaagc actgcaaatg cccgtgccgc acgccgcgct gggcgataac
      481 gtcatcggca aaatgctgca cgtcgcccat atcacctttc agcacggcga tttccagaca
      541 gtcgtcgtga ttgatatgca catgcagcgt ggcgacggag agatcgtggt gatggtgctg
      601 ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt tcatacacat aggagagcac
      661 cgcgaaacct tgcgtgccgt gctgctgagt ggcctcctgg gccagagcgc tacgcagaat
      721 atcgcggata gcttcggaac gattgttata accacgacgc tggctcaggc tgtccagcgt
      781 ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc atttgcgtta aaccttttct
      841 gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt tttgtagcac acgtccggcg
      901 tcagaggaaa aggttaattt atctcccacc acctgggttt cgacgatttg tccgttgtcc
      961 atcaccatca cccgctgaca gaagcgttcc accaggcgta agtcgtgggt gatgaacagg
     1021 caggcggtgc caaactgttg ttgtagcttt ttcagcaggc gaatgacgcc cgcctgtaac
     1081 acgagatcaa ggttagaaac ggcttcatcc agaatcagta gtttcggttc gaccgccagc
     1141 gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta actgcggtgg gcgtttgtcg
     1201 agaacgctgt catcgagatc gaccgccttc agcatttcgc tggcgcgcgc cagttgttcg
     1261 gattttttca gtgatagcag gtggcgcatc ggttcacgca ggatctcgcg cacggttttg
     1321 cgcggattca cggcgctgat ggagtcctga aataccatct gaatatcgcg gcggaacgct
     1381 ttacgctggg cgcgattgag tttcgccagc ggttcgccac gccagctaat attcccctgc
     1441 gcgggcgatt ctaaacccac cagcaaccgc gcgagggtac ttttcccgca gccgctgcgc
     1501 cccagcaggg cgacggtttc gccgcttttc agggtcaggg aaacgttatt cagcaccgcc
     1561 tgatgttgat gttttccgtt aaatccaccg tgcgcatagt gatgggaaag gccggagatg
     1621 ttaagtaaag tcatgatgcc agctccatac cgtagagggc gagatgagcg gaaaccaggc
     1681 tgcgcgtcac tgtatgtttg ggggcgttaa acagcgtttc tacatcgccc tgttcgacaa
     1741 tcttaccgtc agacatcacc gccacgtcat ccgccagacg cgccacaacg cccatatcat
     1801 gggtcaccag cagcattccc ggcgcttgtt tttgcataat gctttccagc agatcgagga
     1861 tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc atcggcgatg ataaacggtg
     1921 attcacacag caccgccatc gcaatcatca tgcgctgcaa catgccgccg ctcatctcga
     1981 acgggtacag cttcagcacg cgcgcggcgt tttccagccc caccgcttct atggcagcgg
     2041 taagcgtagc gtcatcggcg ggtttcccta acgccaggca ggtttcccgc gcgtgggtgt
     2101 gcatggtgtg cagtggatta aaggcgctgc gcgggttctg catgatggtg gcaattttga
     2161 tgccgcgcag ggcgcaaggc gaaaccggtt tgccatcggc taaaatttcc cccgccgtct
     2221 ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca ggttaatgat ttcccgctgc
     2281 cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag ggttaacgat acaccgtgta
     2341 ccagcggctg cgcggcctgt agcgcgatat tacgtagttc aatctgttgc ggcattagtg
     2401 tgcgtgctcc gtcaccagat gagggtccag atgatcgcgc agtgcgtcac ccaccaggtt
     2461 aaaggccatc acgctgataa acagcgccag ccccggccag aacatttgca gcggctgggt
     2521 ccagatatac tggcgcgcgt cgttaatcat cacgccccat tcggcggtcg gcgcggtcac
     2581 accgaggcca aggaaagaca tccccgcgac gtgcagcatc atatggccga tatccagcgt
     2641 tgccagcacc agcagcgaag ggatcaccgc gcctgccaga tgatcgacaa acacccgcac
     2701 atggcccgca ccggaaagcc gtgacgccag cacaaactcg cgttggcgta gtgaaatcac
     2761 caggctgcgc accatgcgtg cataccacgc ccagtgcgac agggcgatgg cgataattac
     2821 gttggtcagc ccggtgccga gcacgccaac cataaagaac gacagaatcg aggtcgggaa
     2881 ggtcataaac atatcggcga cgcgcatggt ggcctgatca acgcgcccgc caatcaaccc
     2941 ggcgctgccg ccaataacca gccctaatgt cagcaccagc agcaggcagg ccattaccga
     3001 accgagcgac acgcgggtcg ctgtcatcag ccgcgagaaa atatcgcgac ctaagtgatc
     3061 ggtgcccagc cagtgctgcg catccggcga aagcaggcgc gacggcaaat caatcgcctg
     3121 ggggtcatac ggcagccacc actggctggt gagcgcaatc agcgccagca gggcgataat
     3181 gatcagcgcc aggcgtaccg accagcggga agagaggaaa aagttcacgc gtgcgctcct
     3241 tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca aatcgacaat caaattacag
     3301 accacaaaaa ccaccaccat catcagcgta aagcactgga tcaccggata gtcacggtta
     3361 aaaatcgccg acaccgcata gcgcccgacg cccggccagg caaagatgtt ttcgataatc
     3421 atcgtcccgc caatcagttc gccgatatgc atccccacgg cggtgatcat cggcagcgag
     3481 gcattgcgca ggatgtggcg acgttcggtc tgtttgtcgt tcaggccgcg cagacgcgcc
     3541 caggtgacgt gacgctgacc ggcgacgtcc agcatactgg cgcgcagtaa acgcgcgtta
     3601 atcgccagcg acataaaggc aatggaaacc gcaggcaaaa tgatgtgctg ccagccgccg
     3661 taacccatcg cgggtagcca ttgcagatac accgaaaacg ccatcaccag taaaaacgcc
     3721 agccagaagt taggcatcga cacgccaaga aacgcgatga aacgtacggc gaaatccggc
     3781 agacggtcgc gatggcgcgc cgcccagata ccgagcggta cggaagtgag cagaattaat
     3841 accagcgccg cacctgcaag ttccagcgtg gcgggcagga agttcagcat atcgtccagt
     3901 accgggcgtt ggctggcgaa tgagatacca aagtcaagat gcagcgcctt ccacaaccag
     3961 gtaccgtact ggacgtacag cggctgatcc agcccgagca tggtgcgggt agaggccagc
     4021 atctccggcg tcggcggcag gttagataaa cgcagataat cgagcgccgg gtcgccggta
     4081 ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga gcaccatcgg gatcagcagc
     4141 agaaagcggc gtaatacgta acgcaacatt aaggtttcac cggtttaatc tgttcgaacg
     4201 gaatttcggt ggcgatcggc gcgtagggga tgttacccag ctccggtttt gataccacca
     4261 tcattgagat gtaactgata ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt
     4321 cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt
     4381 tatcaatcag cggtttgtcg gctaatcctt gctgtgcctg gaagtcagcg tgtgacggta
     4441 cgcgcattga actgaggaag gcgtgtggat catatggcgc gccccaggtg cggtggaaaa
     4501 tcatgccaaa acgaccgtcg cgctgacgag catagatact gctctcttct tcgccaatca
     4561 gcgagacatc tgcgccaatc tggcgcatat cagcctgaat gatttccgcc atcgatttgc
     4621 ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag cggctgacca tttttctcgc
     4681 ggatgtcttt gcccgcaggc agcgtccaac cggctttttc cagcaacgct ttcgctttct
     4741 gcggatcgta ctggcgcggt ttcaggccga ggttggcgta gggcacagaa ggggcaaaca
     4801 gggtgtcggc gacctgctgg gtgccataca acgcgttatc aatcagcgat tttttgttta
     4861 ccgcgtaatt aagagcttca cgtactgcca gctcgttggt gggggctttg gcggtattga
     4921 gcgccagcat cacggtttcg atcggctgtg acagttgggt gtggtaagcc ggattctggc
     4981 taaagcgggc gaaggtatcg agcggtaata acccttcgtt tccgtacagc aggtcgatat
     5041 cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg gatgacgtta aaggtgatct
     5101 ttttaatcgc tggcttttcg ccccagtagt tttcgttacg gacgaagaca tcgtactgat
     5161 tcagtttcga ttcctgcaaa atccacggtc cggtgccaat cggcgcttta attccgttca
     5221 tggtttcatg gtttttaaac tgcgagggag cgataaaacg gaaaggacgg ggcagggcca
     5281 gttcttgcag gaaaggatag taggcgcttt tcagggtaat ttgcagctct gttttactga
     5341 gtgctttaac atcaacaatc tggtttgcca gctccagcca ggcgtgacgt tgacggttat
     5401 cgagcactgc gcggaagttt tctgccgccg cctcggcatc gaacggttca ccgttggaga
     5461 atttcacgtc atcacgcagg gtgaaggtcc aggttttacc atcttctgaa tgagtccagc
     5521 tttttgccag ccacgggatc accgaaccgt ctgcctgata tttcaccaat ggttcataaa
     5581 ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat
     5641 tcaccggcca ggcggtggtg atttcatctg gtgcagcggc atggacgata aaagacgcac
     5701 aagccagcag cgcaaataga gtgcggcgga gtgtggagag catggtaacc ccaatggatt
     5761 aaaatagatg gcggaaataa gtatgacgat tttaagtatt cgtcatactg attacctgtt
     5821 ctggatcaat agatgggcag tcgggtgggc ggatcagtta actgaatcga tccattgcac
     5881 actgtcggcg gtgagcgtaa agggagtagg ggtgcagatc gccaggctta aattttcaag
     5941 ctgacaatgg ctgactgaca gcgaggagtg ataggtgctg tctacgctga cgatttgcca
     6001 ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc gtccagatgc gccagaacat
     6061 ttccagttgc tgatcaggat gcacggcgtc catctcagcg tgttccccga ggctgaatac
     6121 ggcgttcgcc agccagtgcc agttggcgcg cgggcgaatc acttcgatat cgcagccgac
     6181 ttcgccttca tcactcaaca gcagggcgat atcgtcaccg ctatggctta agttgaacca
     6241 tagcggcatt tccggcgcaa atgcaggttt gccttgttcg ccatagatga tctccggtag
     6301 cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc cagcgttcgc gtcgtggacc
     6361 ttgcggtgct tgctcgcgta aacccggtgg cagtggagct gcgcttaagg tcgaaacttt
     6421 ccccagaact atccgataca tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc
     6481 gtatcacttg tcgcctcatc ccggtaaccg acttttcggt ctgcctggcc ccagtaaaat
     6541 cgccagtttg ctaccgcctt tggtggtttc catccagatt ttacacacac tggttaatgg
     6601 caccgaaagt agcatcccta ccgggccgag cagccatccc caaattaaca atgaaagaaa
     6661 taccaccatg gtggacatcc ccaggcgatg gcccatcatc cgtggttcta aaatattgcc
     6721 gatgaccata tggaccacta aaaacaatgc gccgaccaga atacattcgt aaacaccatt
     6781 aaacagcagc acctgaatca ttggcggtac ggcggaaatt accgcgccga tattgggcac
     6841 gtagttgagc aaaaacgcca gtactgccca catcagcgca aactgcaccc ccatcagctc
     6901 cagccccagc cagacgatga cacctgtcca taaactgagt agcgtcttca atgcaagata
     6961 gtgcgaaacg cctttaagtg cgcggtgtaa tcctgcgatg tgaatctgtg ggttattcag
     7021 cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac agcataaaaa ctacggtcat
     7081 caccagcaaa agcacgctcg ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa
     7141 ggtaaccact ttttccgagt ccatccgctg cagcattcgc tccggcgaca tatgcaaatt
     7201 aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc gtcaactcct tattaaactt
     7261 cggcagcata gagataaatt cgttaaacga tgccgccagt acgccgacca gcgcagttag
     7321 tgcgatcagc atcaccacca ccacaatcgt aatggcaatg gggcgttgta ctccccgacg
     7381 aataaaccag gtgacgagcg ggttgaggac gatggcaaaa aacagcgcca gtaacagctg
     7441 cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg aggccagctt
     7501 gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt tccattgggg cttccttgtg
     7561 actttttgta ttaagtgtag cgggagtcac gccagcaata ttctgattct tactgctgat
     7621 tttcacgtca gcacgtggta aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc
     7681 ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc attgtctcca
     7741 ttgtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct gtattaccgg
     7801 gctatgtcca tgatgtgatg ggctttagcg ctttctgggc aggattggtt atcagcctgc
     7861 aatatttcgc caccttgctg agccgtcctc atgccggacg ttacgccgat ttgctgggcc
     7921 ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg ggatatctga
     7981 cggcaggatt aaccgccagt ctgcccgtca tcagcctgtt attactttgc ctgggacgcg
     8041 tgatccttgg gattgggcaa agttttgccg gaacgggatc gaccctgtgg ggcgttggcg
     8101 tggttggctc gctgcatatc gggcgagtga tttcgtggaa cggcattgtc acttacgggg
     8161 cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc ttgcaggcgt
     8221 tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc ccgcgtccga
     8281 cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg cgcgtctggc
     8341 tgtacggtat ggcgctggca ctggcttccg ccggatttgg cgtcatcgcc acctttatca
     8401 cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct
     8461 gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taaccgtatc ggtggcttaa
     8521 acgtagcgat gatttgcttt agcgttgaga taatcggcct gctactggtt ggcgtggcga
     8581 ctatgccgtg gatggcgaaa atcggcgtct tactggcggg ggccgggttt tcgctggtgt
     8641 tcccggcatt gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag ggggcggcgc
     8701 tggcaactta caccgtattt atggatttat cgcttggcgt gactggacca ctggctgggc
     8761 tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga ctggtcacaa
     8821 tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc cctgaggccg
     8881 cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc tgcggtggtc
     8941 tgcgcttttt gctgatcgtc agctggcaga gtaatttgca tggtcagcag ttgattaccc
     9001 acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat aatactgtct
     9061 aactgctgca ttttgtgacc tttcagctca atggctttat tggttaccac ttgcagctgc
     9121 ggatcgcggc tacgttgctg atcttccaga cgcttcgcca gcaccgccag atcttctttt
     9181 ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga ccagacatgc
     9241 atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc tggcagcgag
     9301 aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact ttccgcgacc
     9361 gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc cataaccagc
     9421 aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc gataaacggc
     9481 cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta actcgcctgc
     9541 aaagcgttga tttcggattt atctgccagt cttttcaaca gcatattgag taatacgcca
     9601 tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc gaccagcgcg
     9661 atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc aatgaagaaa
     9721 aaggccagcg tgtcgctgac gttaccgaac agtgtggacg ctgtcggtgc cagccaccag
     9781 cggcgacttt gacgcaggcg gttaaaaacg tgcacgtcga ggatttgccc cagcgcgtag
     9841 gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc gagtgcgccg
     9901 aatccctgcc atgaacccat atagaacagc gacgagatga cataggagat taataacgca
     9961 gggatcatta ccgcgaagat aatgcgtcgg gccagcggtg cgccaaaaat acgcacggtc
    10021 aggtcggtag caagaaaaat aaacggaaag ctaaacgcgc cccaggtggt atggaaacca
    10081 aaaatggaga cgggaagctg aaccagatag ttactggagg tgatcaccag cagatgaaat
    10141 aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt catattgtac
    10201 ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct gttaccttaa
    10261 cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta cgttgacaat
    10321 gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca cagggcgtaa
    10381 actagcgccg tttttttatg tgatgagaag aaaatgaccg atctcttttc cagccctgac
    10441 cacacactcg acgcacttgg cctgcgctgc ccggaacccg taatgatggt gcgcaaaact
    10501 gtgcgcaata tgcagcccgg cgaaacgttg ctgattatcg ccgacgatcc ggccactacc
    10561 cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgcgaa agagacggat
    10621 ggactgcctt atcgttattt gattcgtaaa ggcggttgat gggggctgat tggcttcgat
    10681 gccgcctttt cccctcaccc taaccctctc cccagagggg cgaggggacc gatcgcgctc
    10741 aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt cgctgtcacc
    10801 agcaccgtcg cacccgtatc tgccagcacc gccagccata gtccggtcat ccctaacagc
    10861 gtggtgacga ggaagatccc tttcagtccc agcgcaatgg tgatgttctg gcggatattg
    10921 gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg gttatgggtt
    10981 aatgctgcat cggcggtttc cagcgccacg tctgtgccgc tgcccattgc aatcccaatg
    11041 gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac cgaccatcgc cagcggcgca
    11101 tgttgattca gcttggtcac cgctttgact ttctcttccg gcaataggcc cgctttaaac
    11161 tccagcccca gctccccggc gattgccgcc gctgcgcgtg gattatcgcc ggtgaggatc
    11221 actcctttaa ctcccagcgc gttcagttca ctgatggcag ttgcagcgtc ggcgcgcagg
    11281 gtatcctgca atgcaatgat acccagcacg tcatcattac gtactaccag cacaaccgtt
    11341 tgcccggcgc tttccagttc attaatcaaa ccagcaaatg catcagcggg atgtttcccg
    11401 gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt caatgccaga cccgaccagc
    11461 gcccgctgtg attcggcggt gggaatggcg agttcagcaa cctgtgcttc tcgcacgatg
    11521 gcctgcgcca gtggatgcgt cgcaccttgc tcgaccgccg ctgccagtgt cagcagttca
    11581 gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc gacggtcagc
    11641 gtaccggttt tatcaaacgc cacctgggta acacgaccta gttgttccag cgccgctccg
    11701 cctttaatta acgccccacg acgcgctgct gccgccagcc cggaggtaat cgccgcgggc
    11761 gtggagataa ctaacgcaca cgggcagcca atcagcagca gcgtcaggcc tttataaatc
    11821 cactcttgcc agctggcggc aaacagcagc ggtggcacca gcgtcaccag cagagcgacg
    11881 gccataatcg cgggcgtata gatacggctg aaacggtcga taaaccgctc aatgggtgcg
    11941 cgacgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct ggcacccggt
    12001 tctgacagca cttccagcgt taccagacgg tctacgctgg tggctccagc agggacttta
    12061 tcgcccgttg cgcgctccac cggaatagat tcgccggtca gggcgctttc atcaaaactg
    12121 gcaaacggtg agagcagttt accgtcggca ggcaaacgtc cacctgcggc gacttcaatc
    12181 acatcgccag ggcgcaggct gttaatcgcc acctcttccc gctcaccgtt acgcaggcgc
    12241 gtggcggttt ctggtttcag cgccattaac gcgctgaccc cttgacgtgc gcggctggcg
    12301 gcccagcctt ccagtcgttc gccaatcaaa aacagcagca acaccatcgc agcttcagcc
    12361 gttgcgccaa taaaaagcgc accaatagcg gctacgctca ttaaggtttc aatggcgaag
    12421 tagctgccgg atttgatcaa ccgtaatgcc tgacgagcaa tcgggtacag cccaaccagt
    12481 gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag accccagctg
    12541 attgccatca tcacgattag cgtaatcagc ggcagattct ctttcaggcg tgatgcttgc
    12601 ggttcgtcgg cggactgttc atcgcgcagg gagtagcccg ctttttgcac cgcagattca
    12661 acctgggcac ggatgtcatt gtcggcatca accaccagtt tttcggtggc gaacaacacc
    12721 tgtacctgat tcacgcctgc aagctggcgc acggcatttt ctaccttgcg cgcacaggcg
    12781 gcgcagtcca tgccgctgac tttccagcta tagcgggtgc cggagacgtt ttcagagaga
    12841 gttggcgtgc tggaacatgc gccgtcgcag caacagtcgt tggcgttctg taccgtggtt
    12901 agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg agtcgacatg
    12961 gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag tcgactccag
    13021 agtcaagttt tatcagagat acagcgagcg gacgatcagg aagtgaccgg caaagtagca
    13081 ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtgactac ccagccagac
    13141 aaagttactg ataaacagca acgaagcgcc gacaaacgca gagagcgccg gagcggtcgg
    13201 acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc cgataaacgt
    13261 gcagataggc caacgatact cttccagccg cgtccagata atcgccagta acagcgcgcc
    13321 cagaaccagc agcaccagtg gcagaggcca gaagaaagag agcgtcatct gactggcgaa
    13381 atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca tcagacgttg
    13441 acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccccg ccagcaccag
    13501 atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga gaagaagggt
    13561 taatggttta aatacccagc gttgccaggt tggcccacga tacgatgcat ccacagatag
    13621 ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt gaattcgtta
    13681 ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag ggtggacgga
    13741 tgacaacacc ataactggca gggtatgctt aattccgcat tttccgatga gggataaaga
    13801 tgagcaaacc acctcttttc tttattgtta tcattggctt aatcatcgtc gccgcatcgt
    13861 ttcgttttat gcaacaacga cgggaaaaag ctgataatga tatggcacca ctccagcaaa
    13921 agctggtgtt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt tcgcgccagc
    13981 aggaagtgac tccggcaggc accagtatgc gctatgaggc aagcttcaaa ccgcaaagcg
    14041 gaggaatgga gcagacgttt cgcctcgacg cccagcagta ccacgccctg acagtgggcg
    14101 ataaaggtac gctgagctat aaaggaacgc gctttgtcag ctttgtaggc gaacaataag
    14161 gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg ccgaaaagga
    14221 aaatccgtac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa gtggatttca
    14281 atagcgccag ctgcatacca tgcatcagca cggtaaaaat cagcgccacg ttaacgaaaa
    14341 tattcagcgg acgtgggaag ggatgcacca ggttgaggat taaaaatccc caaacacaga
    14401 gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc gcgttgatac
    14461 agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt tgccggaaca
    14521 gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc agccagccag
    14581 ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa cggtggatcg
    14641 acaaacacga tattatgcgg tgtacctttt tgcgccagga atgacatcgc gttgctgttc
    14701 accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg ctgagaaacc
    14761 gcgcgatcca tctcaatcaa cgttgccccc gcagcatagc gcgacaacgc ttccagcccc
    14821 agcgcgccgc ttccggcgaa gcaatccaga cattgggcgt caacaatgac cggagccagc
    14881 cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg gctatcagga
    14941 accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc gctgccggaa
    15001 tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac ttgcaggcgg
    15061 tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc tatgtacata
    15121 gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg cgaaagaaaa
    15181 aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc cggaaaaaga
    15241 gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc aagagcctgt
    15301 gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg catactgaag ccgaggcgga
    15361 aacttttgct gccgacgttg tggaagtcac tgaacaggtt gctgaaagtg aaaaagcgca
    15421 gcctgaagcg gaagtcgttg cacagccgga accggtcgca gaagaaacgc cggagccagt
    15481 ggctatcgaa cgtgaagagc tgccgttgcc agaagacgtc aacgccgaag aggtttcgcc
    15541 agaagagtgg caggctgaag cggaaaccgt agagattgtc gaagcggcag aagaagaagc
    15601 ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg ccgaagcggc
    15661 agaagaggcg gtgatggtag ttcctccggc agaagaagag cagccggtgg aagaaattgc
    15721 ccaggagcaa gaaaaaccga ccaaagaagg tttcttcgcg cgcctgaaac gcagcctgtt
    15781 aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta aaaaaatcga
    15841 cgatgatctg tttgaggagc tggaagaaca gctgttgatc gccgatgtgg gcgtggaaac
    15901 cacacgtaaa attatcacca atctgacgga aggcgcatcc cgcaagcagc ttcgtgatgc
    15961 cgaggcgctc tatggcctgc tgaaagaaga gatgggcgag attctggcga aagtcgatga
    16021 gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg atggtgggcg tcaacggtgt
    16081 gggtaaaact acgacgattg gtaagctggc acgtcagttt gagcagcagg gtaaatcggt
    16141 gatgctggcg gcgggcgata ctttccgtgc agcagcggtt gaacagcttc aggtctgggg
    16201 tcagcgcaac aatattccgg tgattgccca gcataccggt gcggattccg cctctgttat
    16261 cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg ccgataccgc
    16321 cggacgcctg cagaacaaat cgcacctgat ggaagagttg aagaaaatcg tccgcgtgat
    16381 gaagaaactc gacgttgaag cgccgcatga agttatgctg actattgatg ccagtaccgg
    16441 gcagaatgcg gtaagccagg ccaaactgtt ccacgaagcc gttggcttaa ccggcatcac
    16501 gctaacgaaa ctggacggca cggcgaaagg cggggtaatt ttctcggtgg ctgaccagtt
    16561 tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc gtccgtttaa
    16621 ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat tcgctttgaa
    16681 catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac gttccatatg
    16741 cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag taccctcctg
    16801 aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag cggccatgac
    16861 atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg catgattttc
    16921 caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat cccgctgatt
    16981 atcgctggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcact ggataaagtc
    17041 gggctactgg acaaagcgaa gaacttccct attcagcttt ccggcggtga acaacagcgt
    17101 gttggcattg cccgcgcggt ggtgaataag cccgcggtac tgctggcgga cgaaccgacc
    17161 ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc tgtttgaaga gtttaaccgc
    17221 gttggggtaa ccgtattgat ggcaacgcac gacatcaacc tgatttcacg gcgttcctat
    17281 cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga ataagcgcga
    17341 tgcaatcaat catattcggc agtttggcgg gcgtcttgat cgcttccgta aatcggtcgg
    17401 cggctctggc gacggcggtc gtaacgcccc gaaacgcgcg aaatcctcgc caaaaccggt
    17461 aaatcgtaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg gcgcattgca
    17521 ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg ccatctccct
    17581 gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aatcaggcgg cgacgcagta
    17641 ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg ctggacgatg acgccgctgc
    17701 gggcgtggtg gcacagttgc aggccgagca aggcgtggag aaagtgaact atctttctcg
    17761 tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc tggatatgct
    17821 ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt ttcaggggac
    17881 ggaatcactc aatacgctgc gtgatcgtat cacgcagatt aacggcattg acgaagtgcg
    17941 gatggatgac agctggtttg cccgtctggc ggcgttgacc gggctggtcg ggcgcgtttc
    18001 ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc gtcatcggca acagcgtacg
    18061 tctgagcatc tttgctcgcc gtgactccat caacgtgcag aaactgattg gtgcgacgga
    18121 tggtttcatc cttcgcccgt tcctgtatgg tggcgcactg ctgggatttt ctggcgcatt
    18181 gttgtcatta attttgtcag aaattctgat gctgcgattg tcatcggcgg ttgcggaagt
    18241 ggcacaggtt ttcggaacga agtttgatat caatggttta tcgttcgatg aatgcctgct
    18301 attgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca cggtacaaca
    18361 tttacgccac tttacgcctg aataataaaa gcgtgttata ctctttccct gcaatgggtt
    18421 ccgtagcagg gaaagagatc ccgttgtctc ttcccggtat ttcatctcta tgtcacattt
    18481 tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcacg gtctgataaa
    18541 acagtgaatg ataacctcgt tgctcttaag ctctggcaca gttgttgcta ccactgaagc
    18601 gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt ttagctttag
    18661 ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg atgttgtcgg
    18721 ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg gaagcagcta
    18781 aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat tatgcgggct
    18841 atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg aaagcagtgc
    18901 gccgtttcaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgttcac tggatcaaag
    18961 cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg accaccaaag
    19021 cgcagcgcaa actgttcttc aacctgcgta aaaccaagca gcgtctgggc tggtttaacc
    19081 aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac gttcgtgaga
    19141 tggaatcacg tatggcggca caggacatga cctttgacct gtcttccgac gacgattccg
    19201 acagccagcc gatggcaccg gtgctctatc tgcaggataa atcatctaac tttgccgacg
    19261 gcatcgaaga tgataactgg gaagagcagg cggcaaaccg tctgaccgac gcgatgcagg
    19321 gtctggacga acgcagccag gacatcatcc gtgcgcgctg gctggacgaa gacaacaagt
    19381 ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgaacgtgtg cgccaactgg
    19441 aaaagaacgc gatgaaaaaa cttcgcgccg ctattgaagc gtaatttccg ctattaagca
    19501 gagaaccctg gatgagagtc cggggttttt gttttttggg cctctacaat aatcaattcc
    19561 ccctccggca aaacgtcaat ccccacgcag attgttaata aactgtcaaa atagctattc
    19621 caatatcata aaaatggggt atgttttagc agagtatgct gctaaagcac gggtagtcat
    19681 gcataaaacg aaataaagtg ctgaatcaca acaccacaac acacgtaata accagaagaa
    19741 tggggattct caggatgaat ataaagggta aagcgttact ggcaggatgt atcgcgctgg
    19801 cattcagcaa tatggctctg gcagaagata ttaaagtcgc ggtcgtgggc gcaatgtccg
    19861 gtccggttgc gcagtacggt gaccaggagt ttaccggcgc agagcaggcg gttgcggata
    19921 tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat cgtaaaatat gacgatgcct
    19981 gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt taacgacggc attaaatatg
    20041 tgattggtca cctctgttct tcatcaacgc agcctgcgtc tgatatctac gaagacgaag
    20101 gcattttaat gatcacccca gcggcaaccg cgccggagct gaccgcccgt ggctatcagc
    20161 tgatcctgcg caccaccggc ctggactccg accaggggcc gacggcggcg aaatatattc
    20221 ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga caaacagcaa tacggcgaag
    20281 gtctggcacg agcggtgcag gacggcctga agaaaggcaa tgctaacgtg gtgttctttg
    20341 atggtatcac cgccggggaa aaagatttct ctacgctggt ggcgcgtctg aagaaagaga
    20401 atatcgactt cgtttactac ggcggttacc acccggaaat ggggcaaatc ctgcgtcagg
    20461 cacgcgcggc agggctgaaa actcagttta tggggccgga aggtgtggct aacgtttcgc
    20521 tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg aagaactacg
    20581 atcaggttcc ggcaaacaaa cccattgttg acgcgatcaa agcgaaaaaa caggacccaa
    20641 gcggcgcatt cgtttggacc acctacgccg cgctgcaatc tttgcaggcg ggcctgaatc
    20701 agtctgacga tccggctgag atcgccaaat acctgaaagc gaactccgtg gataccgtaa
    20761 tgggaccgct gacgtgggat gagaaaggcg atctgaaagg ctttgagttc ggcgtatttg
    20821 actggcacgc caacggcacg gcgaccgatg cgaagtaatc attaatcggc aactttgggt
    20881 tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg taaacgcctt
    20941 atccggccta caagatccaa agaaatcagt aaattgcaac acacattgta ggcctgataa
    21001 gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact tgacgattaa
    21061 cgcttctccc agccgccctg ttgcgccgta aaccccagcg cctgcataaa cgccgtcatc
    21121 acaccgcgat cttccaagcc tgcatccgcc atccaccagc atgaaacgcc aggattgtta
    21181 cgcaaaacct cttccagcag atattgcccc acaccgcgac gtcgggtgac ttcccgcaca
    21241 cgcagggaat ccagtgctcc ctcggtgccg cttaaggtta cccgtacggc agcgagcagg
    21301 cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt caacctgtaa agaggaaggg
    21361 gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa gttttctaat
    21421 cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac aaaagtggaa ccagtgtagc
    21481 gaaataattt aatcggaggc tttctctttt ttatttcttt tggcaggtga ttaatttttt
    21541 aacagcaata attacaaagt taaaacatta gagaatgaaa aatacccagc ataatcccct
    21601 gaatggtagt gaattattcc gccctttgtg ccgttatttt atgctgacaa aggcactttt
    21661 ttctgtttat ctatcaataa attcagaata ttatctgttc ttaatcgact gaaaaatagg
    21721 gattttaatc actattatca caaaatactg cgctaaaccc ttaatcagac aggcaaaaac
    21781 agtgcagtat aaaaaaagaa cagtctgatt tgttaacaca taaaaacaaa gcaacacaac
    21841 atcacgaatg gggatttttg actatgaaac ggaatgcgaa aactatcatc gcagggatga
    21901 ttgcactggc aatttcacac accgctatgg ctgacgatat taaagtcgcc gttgtcggcg
    21961 cgatgtccgg cccgattgcc cagtggggcg atatggaatt taacggcgcg cgtcaggcga
    22021 ttaaagacat taatgccaaa gggggaatta agggcgacaa gctggttggc gtggaatatg
    22081 acgacgcctg cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt aatgacggca
    22141 ttaaatacgt tattggtcat ctgtgttctt cttctaccca gcctgcgtca gatatctatg
    22201 aagacgaagg tattctgatg atctcgccgg gagcgaccaa cccggagctg acccaacgcg
    22261 gttatcaaca cattatgcgt actgccgggc tggactcttc ccaggggcca acggcggcaa
    22321 aatacattct tgagacggtg aagccccagc gcatcgccat cattcacgac aaacaacaat
    22381 atggcgaagg gctggcacgt tcggtgcagg acgggctgaa agctgctaac gccaatgttg
    22441 ttttcttcga cggcattacc gctggcgaga aagatttctc cgcgctgatc gcccgcctga
    22501 aaaaagaaaa catcgacttc gtttactacg gcggttacta cccggaaatg gggcagatgc
    22561 tgcgccaggc ccgttccgtt ggcctgaaaa cccagtttat ggggccggaa ggtgtgggta
    22621 atgcgtcgct gtcgaatatt gctggcgatg ctgccgaagg catgttggtc accatgccaa
    22681 aacgctatga ccaggatccg gcaaaccagg gcatcgttga tgcgctgaaa gcagacaaga
    22741 aagatccgtc cgggccttat gtctggatca cttacgcggc ggtgcaatct ctggcaacag
    22801 cccttgagcg taccggcagc gatgagccgc tggcgctggt gaaagattta aaagctaacg
    22861 gtgcaaacac cgtgattggg ccgctgaact gggatgaaaa aggcgatctt aagggatttg
    22921 attttggtgt cttccagtgg cacgccgacg gttcatccac ggcagccaag tgatcatccc
    22981 accgcccgta aaatgcgggc gggtttagaa aggttacctt atgtctgagc agtttttgta
    23041 tttcttgcag cagatgttta acggcgtcac gctgggcagt acctacgcgc tgatagccat
    23101 cggctacacc atggtttacg gcattatcgg catgataaac ttcgcccacg gcgaggttta
    23161 tatgattggc agctacgtct catttatgat catcgccgcg ctgatgatga tgggcattga
    23221 taccggctgg ctgctggtgg cggcgggatt cgtcggcgca atcgtcattg ccagcgccta
    23281 cggctggagt atcgaacggg tggcctatcg cccggtgcgt aactctaagc gcctgattgc
    23341 gctcatctcg gcaatcggta tgtccatctt cctgcaaaac tacgtcagcc tgaccgaagg
    23401 ttcgcgtgac gtggcgctgc cgagcctgtt taacggtcag tgggtggtgg gccatagcga
    23461 aaacttctct gcctctatta ctaccatgca ggcggtgatc tggattgtca ccttcctcgc
    23521 catgctggcg ctgacgattt tcattcgcta ttcccgtatg gggcgcgcgt gtcgtgcctg
    23581 cgcggaagat ctgaaaatgg cgagtctgct tggcattaac accgaccggg tgattgcgct
    23641 gacctttgtg attggcgcgg cgatggcggc ggtggcgggt gtgctgctcg gtcagttcta
    23701 cggcgtaatt aacccctaca tcggctttat ggccgggatg aaagccttta ccgctgcggt
    23761 gctcggtggg attggcagca ttccaggggc gatgattggt gggctgatcc tggggattgc
    23821 ggaggcgctc tcttctgcct atctgagtac ggaatataag gatgtggttt catttgccct
    23881 gctgattctg gtgctgctgg tgatgccgac cggtattctg ggtcgcccgg aggtagagaa
    23941 agtatgaaac cgatgcatat tgcaatggcg ctgctctctg ccgcgatgtt ttttgtcctg
    24001 gcgggcgtct ttatgggcgt gcaactggag ctggatggca ccaaactggt ggtcgacacg
    24061 gcttcagaca tccgttggca gtgggtgttt atcggcacgg cggtggtctt tttcttccag
    24121 cttttgcgac cggctttcca gaaagggcta aaaagcgttt ccggaccgaa gtttattctg
    24181 cccgcgattg atggctccac ggtgaagcag aaactattcc tcgtggcgct gctggtgctt
    24241 gcggtggcgt ggccgtttat ggtgtcgcgt gggacggtgg atatcgccac cctgaccatg
    24301 atctacatta tcctcggcct cgggctgaac gtggttgttg gtctttctgg tctgctggtg
    24361 ctggggtacg gcggctttta cgccatcggc gcttacactt ttgcgctgct caatcactat
    24421 tacggcctgg gcttctggac ctgtctgccg attgccgggt taatggcagc ggcggccggc
    24481 ttcctgctcg gcttcccggt gcttcgcttg cgcggcgact atctggcgat cgttaccctc
    24541 ggtttcggcg aaattgtgcg catattgctg ctcaataaca ccgaaattac cggcggcccg
    24601 aacggaatca gtcagatccc gaaaccgaca ctcttcggac tcgagttcag ccgtaccgct
    24661 cgtgaaggcg gctgggacac gttcagtaat ttctttggcc tgaaatacga tccctccgat
    24721 cgtgtcatct tcctctacct ggtggcgttg ctgctggtgg tgctaagcct gtttgtcatt
    24781 aaccgcctgc tgcggatgcc gctggggcgt gcgtgggaag cgttgcgtga agacgagatc
    24841 gcctgccgtt cgctgggctt aagcccgcgt cgtatcaagc tgactgcctt taccataagt
    24901 gccgcgtttg ccggttttgc cggaacgctg tttgctgcgc gccagggctt tgtcagcccg
    24961 gaatccttca cctttgctga atcggcgttt gtactggcga tagtggtgct cggcggtatg
    25021 ggatcgcagt ttgcggtgat tctggcggca attttgctgg tggtatcgcg cgaattgatg
    25081 cgtgatttca acgaatacag catgttaatg ctcggtggtt tgatggtgct gatgatgatc
    25141 tggcgtccgc agggcttgct gcccatgacg cgcccgcaac tgaagctgaa aaacggcgca
    25201 gcgaaaggag agcaggcatg agtcagccat tattatctgt taacggtctg atgatgcgct
    25261 tcggcggctt gctggcggtg aacaacgtca atcttgaact gtacccgcag gagatcgtct
    25321 cgttaatcgg ccctaacggt gccggaaaaa ccacggtttt taactgcctg accggattct
    25381 acaaacccac cggcggcact attttactgc gtgatcagca tctggaaggt ttaccggggc
    25441 agcaaattgc ccgcatgggc gtggtgcgta ccttccagca tgtgcgtctg ttccgtgaaa
    25501 tgacggtgat tgaaaacctg ctggtggcgc agcatcaaca actgaaaacc gggctgttct
    25561 ctggcctgtt gaaaacgcct tctttccgtc gcgcccagag cgaagcgctc gaccgcgccg
    25621 ccacctggct tgagcgcatt ggtttgctgg aacacgccaa ccgtcaggcg agtaacctgg
    25681 cctatggtga ccaacgccgt ctggagattg cccgctgcat ggtgacgcag ccggagattt
    25741 taatgctcga cgaaccggcg gcaggtctta acccaaaaga aaccaaagag ctggatgagc
    25801 tgattgccga actgcgcaat catcacaaca ccactatctt gttgattgaa cacgatatga
    25861 agctggtgat ggggatttca gaccgaattt atgtggtcaa tcaggggacg ccactggcaa
    25921 acggtacgcc ggagcaaatc cgtaataacc cggacgtgat ccgtgcctat ttaggtgagg
    25981 cataagatgg aaaaagtcat gttgtccttt gacaaagtca gcgcccacta cggcaaaatc
    26041 caggcgctgc atgaggtgag cctgcatatc aatcagggcg agattgtcac cctcattggc
    26101 gcgaacgggg cggggaaaac caccttgctc ggcacgttat gcggcgatcc gcgcgctacc
    26161 agcgggcgaa ttgtgtttga tgataaagac attaccgact ggcagacggc gaaaatcatg
    26221 cgcgaagcgg tagcgattgt cccggaaggg cgtcgcgtct tctcgcggat gacggtggaa
    26281 gagaacctgg cgatgggcgg cttttttgcc gaacgcgacc agttccagga gcgcataaag
    26341 tgggtgtatg aactgtttcc acgtctgcat gagcgccgtg ttcagcgggc aggcaccatg
    26401 tccggcggtg agcaacaaat gctggcgatt ggtcgcgcgc tgatgagcaa cccgcgtttg
    26461 ctgctacttg atgagccatc gctcggtctt gcgccgatta tcatccagca aattttcgac
    26521 accatcgagc agctgcgcga gcaggggatg actatcttcc tcgtcgagca gaacgccaac
    26581 caggcgctaa agctggcgga tcgcggctac gtgctggaaa acggccatgt ggtgctctcc
    26641 gataccggcg acgcgctgct ggcgaacgaa gcagtgagaa gtgcgtattt aggcgggtaa
    26701 taacacgttg attgataagg agtcaaaaga ctcctttgag acaggtgaca aatgtaaaat
    26761 tgcctgatgc gctgcgctta tcaggcctac tgggtgagtt gcaatatatt gaatttgcac
    26821 gatcttgtag gccggataag gcgtttacgc cgcatccggc atgaaacgat gggtaatctg
    26881 tagagtttga ttcagacctt ctattttccg cttatccgtg ccccatctcc cattttccct
    26941 cacccacgcc gtcaccgcct tgtcatcttt ctgacacctt actatcttac aaatgtaaca
    27001 aaaaagttat ttttctgtaa ttcgagcatg tcatgttacc ccgcgagcat aaaacgcgtg
    27061 aattcgcgca ttcggtacaa caagagagat aaacgatgaa accgttacat tatacagctt
    27121 cagcactggc gctcggactg gcgttaatgg ggaatgcaca ggcagtgacg accattccgt
    27181 tctggcattc tatggaaggg gaactgggta aagaggtgga ttctctggcc caacgtttta
    27241 acgccgaaaa tccggattac aaaattgtac cgacctataa aggcaactac gaacagaatt
    27301 taagcgcggg gattgccgca tttcgtaccg gcaacgctcc ggctattttg caggtttatg
    27361 aagttggcac cgccaccatg atggcatcga aagccattaa accggtatat gacgtgttta
    27421 aagaggcggg gattcaattc gatgagtcgc agtttgtgcc gacggtttcc ggttactact
    27481 ccgacagcaa aacgggccac ttactctccc agccattcaa cagctcgacc cccgttctct
    27541 attacaacaa agacgccttc aagaaagcag gattagaccc ggaacaaccg ccgaaaacct
    27601 ggcaggatct ggcggactat gccgcgaaac tgaaagcctc cggtatgaag tgcggctacg
    27661 ccagcggctg gcagggctgg atccaactgg aaaactttag cgcctggaac ggtctgccgt
    27721 ttgccagcaa aaacaacggc tttgacggca cggacgcggt gctggagttc aacaagccgg
    27781 agcaggtgaa acatatcgcc atgctcgagg agatgaacaa gaagggcgac ttcagctacg
    27841 tcggtcgtaa ggatgaatcc accgagaagt tctataacgg tgattgcgcg atgaccaccg
    27901 cctcttccgg ttctcttgcc aacattcgcg agtacgccaa atttaattac ggcgtaggca
    27961 tgatgcctta cgacgccgat gcgaaagatg cgccacaaaa cgccattatc ggcggagcca
    28021 gcctgtgggt gatgcagggt aaagataaag aaacgtatac cggtgtggcg aagttcctcg
    28081 atttcctcgc gaagccagaa aacgctgccg agtggcatca gaaaaccggt tatctgccaa
    28141 ttaccaaagc ggcgtatgac ctgacccgtg agcagggctt ttacgagaaa aacccaggtg
    28201 cggataccgc gacgcgtcag atgctgaata agccgccgtt gccgttcacc aaagggctgc
    28261 gtctgggcaa catgccgcag atccgcgtga ttgtggatga agagctggag agcgtgtgga
    28321 ccggtaagaa gacaccacag caggcactgg ataccgccgt tgagcgtgga aatcagttgc
    28381 tgcgccgctt tgagaaatcg acgaagtctt aatcagtgta atgtcggatg cgtttcgctt
    28441 atctgacttg gcatcgcgtg taggccggat aagcgaagcg catccggcac agttcaggaa
    28501 ttaaccgtaa tgtcatcatc ccgtccggtg ttccgctcgc gctggctgcc ttatctgctg
    28561 gtcgcgccgc agctcatcat caccgttatc ttttttatct ggcctgcggg cgaagcgttg
    28621 tggtactcgc tacaaagcgt cgatccgttt ggtttctcca gccagtttgt cggcctggat
    28681 aacttcgtcg cgctgtttca tgacagctac tatctcgact ccttctggac gacgataaaa
    28741 ttcagcacct ttgtcaccgt cagcggtttg ctggtgtcgc tgttcttcgc ggcgctggtg
    28801 gagtacatcg tgcgcggcag ccgtttctat caaaccttaa tgctgctgcc ttacgccgtg
    28861 gctcccgccg ttgccgccgt attgtggatc ttcctgttta accccggtcg cgggctgatc
    28921 acccattttc tcgcggagtt cggctacgac tggaaccacg cgcaaaacag cggtcaggca
    28981 atgtttctgg tggtgtttgc ctcagtatgg aagcaaatca gctacaactt cctgttcttc
    29041 tatgccgcgc tgcaatccat tccccgttcg ttgatcgaag ccgcagccat cgacggtgca
    29101 ggcccaattc gccgcttctt taagattgcg ctgccgctta tcgctccggt gagtttcttc
    29161 ctgctggtgg tgaacctggt gtatgccttc ttcgacacct tcccggtgat cgacgccgcc
    29221 acgtccggcg ggccggttca ggccaccacg acgctgattt ataagatcta ccgtgaaggc
    29281 tttaccggac tggatctggc ttcgtctgcc gcgcagtcgg tggtgttgat gttcctcgtc
    29341 atcgtgctga cggtggtgca gttccgctat gttgaaagca aggtgcgtta ccaatgattg
    29401 agaaccgtcc gtggctgacg atattcagcc ataccatgct gatcctcggg atcgcggtga
    29461 tcctcttccc gctgtacgtg gcgtttgtcg cggcgacgct ggataaacag gccgtctatg
    29521 ccgcgccgat gacgctcatc cccggcacac atctgctgga aaatatccac aacatctggg
    29581 ttaacggggt aggcacgaat agcgcgccgt tctggcggat gttgcttaac agctttgtga
    29641 tggcgttcag cattacgctc ggcaaaatta ccgtctcgat gctctcggca tttgccattg
    29701 tctggtttcg ttttccgcta cgtaacctct tcttctggat gatttttatc accctgatgc
    29761 tgccggttga agtacgtatc ttcccgacgg tggaagtcat cgccaacctg aagatgctcg
    29821 acagctacgc cggtttaacg ctgccgctga tggcctcggc gaccgccacg tttctgtttc
    29881 gccagttctt tatgacgctg ccggatgaac tggtggaagc ggcgcggatc gacggcgcgt
    29941 caccaatgcg tttcttttgc gacatcgttt ttccgctctc caaaaccaat ctggcggcgc
    30001 tgtttgtgat cacctttatt tacggctgga accagtattt gtggccgttg ttgattatta
    30061 ccgatgtgga tctcggcacc accgtggcag ggatcaaagg gatgatcgct acaggcgaag
    30121 gcaccactga atggaactca gtgatggcgg cgatgttgtt aacgcttatc cctccggtgg
    30181 tgattgtttt agtgatgcag cgtgccttcg tgcgcggcct ggtcgatagt gagaaataag
    30241 atggcaggac tgaaattaca ggcagtaacc aaaagctggg atggtaaaac ccaggtcatt
    30301 aaaccgctga cccttgatgt ggcggatggc gaatttatcg tgatggtcgg gccgtctggc
    30361 tgcgggaaat cgacgctgct gcgcatggtt gccgggctgg agcgggtgac agaaggcgat
    30421 atctggatca acgaccagcg cgtgactgaa atggagccaa aagatcgcgg gattgcgatg
    30481 gtgttccaga actacgcgct ttatccgcat atgagtgtcg aagaaaacat ggcgtggggg
    30541 ctgaaaattc gcggcatggg caagcagcaa attgccgagc gcgttaaaga agcggcgcgc
    30601 attctggagc tggacggtct gctcaaacgt cgcccgcgcg agctttccgg cggtcagcgc
    30661 cagcgtgtgg cgatgggccg cgcgattgtg cgcgatccgg cggtgttcct gtttgatgag
    30721 ccgctctcta acctcgatgc caagctgcgc gtgcagatgc gtcttgaact gcaacagttg
    30781 caccgtcgcc tgaaaacgac ttcactctac gttactcacg atcaggttga agcgatgacg
    30841 ctcgcccagc gagtaatggt gatgaacggc ggcgttgccg aacagattgg cacaccagtt
    30901 gaagtctacg aaaagcccgc cagcctgttt gtagcgagtt ttatcggcag tccggcgatg
    30961 aacctgctga caggccgcgt gaataacgaa ggcacgcatt tcgaactgga cggcggtatt
    31021 gagctgccgc taaacggtgg ctaccgtcag tatgccgggc gtaaaatgac tctcggcatt
    31081 cgcccggaac atattgcgct aagctcgcag gcagaaggcg gcgtaccgct ggtgatggac
    31141 acgctggaga tcctcggcgc agataacctg gcgcacggac gctggggcga gcagaagctg
    31201 gtggtgcgac tggcgcatca ggagcgcccg acggcaggca gcacgctgtg gctgcatctg
    31261 gcggaaaatc agctgcatct ttttgatggt gaaacaggac aacgagtatg agtaactggc
    31321 cttatccccg catcgtcgct catcgtggcg gcggtaagct ggccccggaa aacaccctgg
    31381 cggcaatcga cgtcggggca aaatacggtc ataagatgat cgaatttgac gcgaagttat
    31441 cgaaagatgg cgagatcttc ctgctccatg acgacaatct cgaacgcacc agcaacggct
    31501 ggggcgtcgc gggtgaactg aactggcagg atttactgcg cgtggatgcg ggcagttggt
    31561 acagcaaagc gtttaaaggt gagccgctgc cgttgctttc gcaggtggcg gaacgctgtc
    31621 gcgaacacgg gatgatggcg aatatcgaaa tcaaacccac cactggcact ggaccattaa
    31681 cgggcaaaat ggtggcgctg gcggcacgcc aactgtgggc cggtatgacg ccgccgctgc
    31741 tgtcatcgtt tgagattgat gctttagaag ctgcacagct ggcggcaccg gaactgccgc
    31801 gtggtttatt gcttgatgag tggcgcgacg actggcgcga actgaccgcg cggctgggct
    31861 gcgtctctat tcatctcaat cataagttac tcgataaagt gcgagtgatg cagttgaaag
    31921 acgccggact gtggattctg gtttataccg tcaataaacc ccagcgcgcg gcagagttgc
    31981 tgcgctgggg cgtggattgc atctgtaccg atgcgattga cgtgattggt ccgaacttta
    32041 cggcccaata gttttcaacg gaatgtcagg ctgcggcgtg ctgggctgat tcagcatgtc
    32101 gccgtttctc ctctccggca gcatatgctg ctgattcaac gaactatccg gattacggtt
    32161 gctgtttaac atcccgccgt tggtgttggg cagcatttgc tgccgggcgg gatttcgctc
    32221 ccccggctgt gactgcaaca cccgctgaga attattgttt atctggtttt ctaaatgctg
    32281 ctgttgcaac tgagtttgcg ttttcagttg ctgattcagc atcccttttt gctggatttg
    32341 ctgagtctgc atctgggttt gcatccgctg ctggctgggt atctgatacc ccggctggtt
    32401 agggttgttc agagtattaa tgggctgtgc aaagccgaca aacggcagga gtgccgtaag
    32461 aatcagaagc cgtttcatcg cgtatcctcc tctgaagata tcctttaagt ttactcgctt
    32521 cccgacaaaa cgatgattaa ttcagagtta tataccaggc ttagctgggg ttgcccctta
    32581 atctctggag aataacgatg ataaaaccga cgtttttacg ccgggtggcc attgctgctc
    32641 tgctctcagg aagttgtttt agcgccgccg ccgcgcctcc tgcgccgccc gtctcgtatg
    32701 gtgtggagga agatgtcttc cacccggtac gcgcgaaaca gggaatggta gcgtctgtgg
    32761 acgccactgc cactcaggtg ggggtggata ttctcaagga gggcgggaat gccgttgatg
    32821 ccgccgtggc ggtgggctac gcgctggcgg taacgcatcc gcaggcaggg aatctgggcg
    32881 gtggtggttt tatgctgatc cgctcgaaaa atggcaatac caccgctatc gatttccgcg
    32941 aaatggcacc cgctaaagcg acccgcgata tgttccttga tgaccagggc aacccggaca
    33001 gcaaaaaatc actcacttcg catctggctt ccggcacacc aggtacggtc gctggtttct
    33061 cgctggcgtt ggataagtac ggcactatgc cgttgaacaa agtggtgcag cccgcgttca
    33121 aactggcacg cgatggtttt atcgttaacg atgctttggc agacgatctc aaaacctacg
    33181 gtagcgaagt gttgccgaat cacgaaaaca gcaaagctat cttctggaaa gagggcgaac
    33241 cgctgaaaaa gggcgacacg ctggtgcagg cgaacctggc aaagagcctg gagatgattg
    33301 ctgaaaacgg cccggacgaa ttctataaag gcacgattgc ggaacagatc gcccaggaga
    33361 tgcagaaaaa cggtggcttg atcactaaag aagatttagc ggcctataaa gcggtcgaac
    33421 gcactccgat aagcggcgat tatcgcgggt atcaggttta ctccatgcca ccgccatcct
    33481 ccggcgggat ccatatcgta caaatcctca atattctgga aaacttcgat atgaagaaat
    33541 acggctttgg cagcgccgat gcgatgcaaa tcatggcaga agcggagaaa tacgcctacg
    33601 ccgaccgctc ggaatatctt ggcgacccgg attttgtcaa agtcccgtgg caggcactga
    33661 ccaataaagc ctatgccaaa tccattgccg atcaaattga tatcaacaaa gcgaagccgt
    33721 ccagtgagat tcgccctggc aagcttgcgc cttatgagag taatcaaact acccattact
    33781 ctgtagtgga taaagacggt aacgcggtgg cggtgaccta tacgctgaac accaccttcg
    33841 gtacgggtat tgtcgcgggc gagagcggta ttctgcttaa taaccagatg gatgatttct
    33901 ccgccaaacc gggcgtaccg aacgtttacg ggctggtggg cggtgatgcc aacgccgtcg
    33961 ggccgaacaa acgcccgctg tcgtcgatgt cgccgaccat tgtggtgaaa gacggtaaaa
    34021 cctggctggt taccggtagc ccaggcggta gccggatcat cactacagtg ctgcaaatgg
    34081 tggtgaatag catcgattat ggcatgaacg tcgccgaagc gaccaatgcg ccgcgtttcc
    34141 accatcagtg gttgccggac gagctgcgtg tcgaaaaagg gtttagcccg gatacgctca
    34201 agctgctgga agcaaaaggt cagaaagtgg cgctgaaaga ggcgatgggc agtacacaaa
    34261 gcattatggt tgggccggac ggtgagttgt acggcgcatc cgacccgcgc tcggtggatg
    34321 atttaacggc ggggtactaa ggttagcggc cctcttcgtg ggaagagggc tattttgtca
    34381 gggcaagccg aaggtagcct tttttatttc gtaatcctgt agatattctt ccagcggctt
    34441 tgccgttccc agagaatgaa tttcaccact gtctttatca ataataaaag gtgcgttacc
    34501 agctaagcgc gcggcctcat ctccagtttc gagaaattct cgtgcttcga aacagaaata
    34561 ccagccctgg ctaaagcgtc catgtagagt aatgacgacc gggagatctg catcatcaag
    34621 gtaatggttc gctttcgcga atgcgtcgtg ataagtaatc ataattataa taaatattat
    34681 tgttgagtgt tatattatta ttcctgtgtg atacattgag caaagacgcg ttcatcttcg
    34741 tcaaagatat tattaaatgc tggttttgaa aaaagttcat cggataagat atagtcacga
    34801 aataagtatc tgctaatatt ttatgatttc tttttcagaa cgtcgaacgg gattttctta
    34861 tttaagatat cagaatctta tgattcgggt aaaattctaa ataaatcaac atgtcattaa
    34921 aatcatcata gccattagct atttcggcta aaatagagac tacatgtctt cggtccatct
    34981 cacttaagga gtgtagttcc gttgtaagtt tttccatagc ttgcactgct aaatttcgaa
    35041 caaggaattt tctgctggta atctctaaaa agatggcatg gtttacaatg atttttgttt
    35101 ccttttgatt attatgaaca actgtccatg atttcgttta agaatg
//
LOCUS       DAZKGS010000030        34573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKGS010000030 DAZKGS010000000
VERSION     DAZKGS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..1272)
                     /gene="amiC"
                     /locus_tag="ACHOQY_001506"
     CDS             complement(19..1272)
                     /gene="amiC"
                     /locus_tag="ACHOQY_001506"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9677635.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1504..2835
                     /gene="argA"
                     /locus_tag="ACHOQY_001507"
     CDS             1504..2835
                     /gene="argA"
                     /locus_tag="ACHOQY_001507"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9677636.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2897..4723)
                     /gene="recD"
                     /locus_tag="ACHOQY_001508"
     CDS             complement(2897..4723)
                     /gene="recD"
                     /locus_tag="ACHOQY_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9677637.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4723..8265)
                     /gene="recB"
                     /locus_tag="ACHOQY_001509"
     CDS             complement(4723..8265)
                     /gene="recB"
                     /locus_tag="ACHOQY_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9677638.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8258..11146)
                     /gene="ptrA"
                     /locus_tag="ACHOQY_001510"
     CDS             complement(8258..11146)
                     /gene="ptrA"
                     /locus_tag="ACHOQY_001510"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9677639.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHLAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11322..14690)
                     /gene="recC"
                     /locus_tag="ACHOQY_001511"
     CDS             complement(11322..14690)
                     /gene="recC"
                     /locus_tag="ACHOQY_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9677640.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14703..15026)
                     /locus_tag="ACHOQY_001512"
     CDS             complement(14703..15026)
                     /locus_tag="ACHOQY_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9677641.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15011..15418)
                     /locus_tag="ACHOQY_001513"
     CDS             complement(15011..15418)
                     /locus_tag="ACHOQY_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9677642.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15415..15978)
                     /locus_tag="ACHOQY_001514"
     CDS             complement(15415..15978)
                     /locus_tag="ACHOQY_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9677643.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(15969..16439)
                     /locus_tag="ACHOQY_001515"
     CDS             complement(15969..16439)
                     /locus_tag="ACHOQY_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9677644.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16623..17417)
                     /gene="thyA"
                     /locus_tag="ACHOQY_001516"
     CDS             complement(16623..17417)
                     /gene="thyA"
                     /locus_tag="ACHOQY_001516"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9677645.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17424..18299)
                     /gene="lgt"
                     /locus_tag="ACHOQY_001517"
     CDS             complement(17424..18299)
                     /gene="lgt"
                     /locus_tag="ACHOQY_001517"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9677646.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18450..20696)
                     /gene="ptsP"
                     /locus_tag="ACHOQY_001518"
     CDS             complement(18450..20696)
                     /gene="ptsP"
                     /locus_tag="ACHOQY_001518"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9677647.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYHHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20709..21239)
                     /gene="rppH"
                     /locus_tag="ACHOQY_001519"
     CDS             complement(20709..21239)
                     /gene="rppH"
                     /locus_tag="ACHOQY_001519"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9677648.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21592..21738)
                     /gene="ygdT"
                     /locus_tag="ACHOQY_001520"
     CDS             complement(21592..21738)
                     /gene="ygdT"
                     /locus_tag="ACHOQY_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9677649.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21924..22613
                     /gene="mutH"
                     /locus_tag="ACHOQY_001521"
     CDS             21924..22613
                     /gene="mutH"
                     /locus_tag="ACHOQY_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9677650.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22682..23395
                     /locus_tag="ACHOQY_001522"
     CDS             22682..23395
                     /locus_tag="ACHOQY_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9677651.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23533..23751
                     /gene="ygdR"
                     /locus_tag="ACHOQY_001523"
     CDS             23533..23751
                     /gene="ygdR"
                     /locus_tag="ACHOQY_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9677652.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23859..24899
                     /locus_tag="ACHOQY_001524"
     CDS             23859..24899
                     /locus_tag="ACHOQY_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9677653.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24931..26124)
                     /gene="lplT"
                     /locus_tag="ACHOQY_001525"
     CDS             complement(24931..26124)
                     /gene="lplT"
                     /locus_tag="ACHOQY_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9677654.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26117..28276)
                     /gene="aas"
                     /locus_tag="ACHOQY_001526"
     CDS             complement(26117..28276)
                     /gene="aas"
                     /locus_tag="ACHOQY_001526"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9677655.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28862..29893
                     /gene="galR"
                     /locus_tag="ACHOQY_001527"
     CDS             28862..29893
                     /gene="galR"
                     /locus_tag="ACHOQY_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9677656.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29900..31162)
                     /gene="lysA"
                     /locus_tag="ACHOQY_001528"
     CDS             complement(29900..31162)
                     /gene="lysA"
                     /locus_tag="ACHOQY_001528"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9677657.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31284..32219
                     /gene="lysR"
                     /locus_tag="ACHOQY_001529"
     CDS             31284..32219
                     /gene="lysR"
                     /locus_tag="ACHOQY_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9677658.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32206..32898)
                     /gene="ygeA"
                     /locus_tag="ACHOQY_001530"
     CDS             complement(32206..32898)
                     /gene="ygeA"
                     /locus_tag="ACHOQY_001530"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9677659.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33027..34445)
                     /gene="araE"
                     /locus_tag="ACHOQY_001531"
     CDS             complement(33027..34445)
                     /gene="araE"
                     /locus_tag="ACHOQY_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9677660.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8447 a         9514 c         8566 g         8046 t
ORIGIN      
        1 ttgaataaat taaaaacatc atccccttct cgccagcgtc gccccatcgg caaaatacgc
       61 tttaatcccc gcaagaatag actccgcaac ttcctgctga aaggtcgccg ttttcagttt
      121 acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg gaatatctgg
      181 tgcctttagt acggcaaacc cggcctgttc aacctgattt ttatgcagct tgttgatttt
      241 accgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatgg tcagcgactg
      301 caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca cgccaccaat
      361 caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg ttgcaccttt
      421 ggttgagagc gcaaacacag aggaaccgct cggctgacga ctggtaaagg cgtcggcatg
      481 gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca cttgcaacgg
      541 aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt tctcgatcag
      601 agagcgcaaa cggcgagcta tttgcaatac cacatctttt tctcgcgttt tgtatttccc
      661 caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa tcggacgatc
      721 gcgccctgct ttacccggtt gtggaccact ttgtgctggc ggcacctgct tttcgaggtc
      781 gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct gtgcattggc
      841 cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa gggcaaacag
      901 ctgcggtttt acgttttgct ttaattcaaa aaccatccgt acggtctgcg ggtcaaattg
      961 cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg ccatcccctt
     1021 gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttccg ggttactcaa
     1081 cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacacggg tgtagctgga
     1141 cgcaggccag acgcgcaccg ccacgacctg gctgaccgca gccagactga cctgacttac
     1201 gctcaatagc cacatggcac ccgcgccttg cagtaaacga cgacggctga ttgcagtgtt
     1261 ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa gtaaacgact
     1321 aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa cggtaaacat
     1381 tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa tttacacttg
     1441 caccctgcga aaaaacagaa taaaaataca ctaatttcga ataatcatgc aaaggggtgt
     1501 gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc ggttccctat
     1561 atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc cattgagcat
     1621 gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg catccgtctg
     1681 gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggccgcgca tcaccacgaa
     1741 ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact ggtgaagcag
     1801 gctgcgggaa cattgcaact ggacatcact gctcgcctgt cgatgagtct caataacacg
     1861 ccgctacagg gcgcgcatat caacgtcgtc agtggcaatt ttattattgc ccagccgctg
     1921 ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat tgatgaagac
     1981 gcgatccatc gtcaactgga cagcggtgca atagtgctaa tggggccggt cgctgtttca
     2041 gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact ggccatcaaa
     2101 ctgaaagctg aaaagatgat tggtttttgc tcttcccaag gcgtcactaa tgacgacggt
     2161 gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc ccaggaagag
     2221 aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa agcctgccgc
     2281 agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag atggcgcgct gttgcaagag
     2341 ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga gcagattcgt
     2401 cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct ggagcagcaa
     2461 ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa attcaccatt
     2521 attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc ggaagagaag
     2581 attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc acggggcgag
     2641 gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa attatttgtg
     2701 ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccagt ggatattgat
     2761 ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa agtgttgatg
     2821 gcggatttgg ggtaagcgat gaaaatcgcc ggatgcgaca tgcgtaacac tcgtgcgtcg
     2881 catcaggcaa ttacgtttat tcccgcgaac taaacaatgc cgccagacca ctgcgccgct
     2941 cagtgcgcgt ggcgattgcc gcacttaata tgcgctcatc ggcatacagc gacagacggc
     3001 gacgcgcgcg ggtcactgcg gtgtaaacca gctctcgcgt tactaccggc gtgcgttggc
     3061 tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt accgtcatcg
     3121 cccacgtcgt ttcgtgctct ggcaggcggc tcggttgcac agacttaata ttgccgtccg
     3181 gcatcgcaaa ccaaacgcgc gtcccctgcc cgcgatccag cgcaataccg atatcgccat
     3241 taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcgacct tcgtaccaac
     3301 gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgctca attcgctcat
     3361 tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagttgg tactcattga
     3421 acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga taacgtccat
     3481 aacccgcaag agcttcctca agcatcgcaa tataatcttc tccgctctgt aaaagtcgtt
     3541 tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta tcaccacggt
     3601 tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag cttttttgca
     3661 gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc ggaacgtgag
     3721 tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg ttggcataag
     3781 cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca cgatcgccga
     3841 gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc ataggcagat
     3901 cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta ccggcatgat
     3961 gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg ctggcatctt
     4021 ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc ttgccgagag
     4081 attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata cggcagcgtt
     4141 cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc gttttaccgg
     4201 tgcctgggcc gccggaaacc accgagatcc gccgcgtcag cgccactgcc gctgcaactt
     4261 tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc gccagtagag
     4321 cttcatcaac ctcaatggca tgattcacct cattgaagaa gcgcgctacc gtccgctcgt
     4381 tacaccacat gcgattcaaa taaagacgat cgccacagag gaccatcggc gtgggttcat
     4441 ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt agctcaccga
     4501 tttcactgac acaggtcgcc aacagcggat gcgacgcctc gttattttcc agtcgtgaaa
     4561 gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc gcgagggtga
     4621 cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc gggcgtagct
     4681 gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct cctccagggt
     4741 cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc gggttgtgta
     4801 aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga taacgccgcc
     4861 aaagtgacga tcatagtcgt aatcagcaat gcgatggcgc agataacgat gcagcgccag
     4921 ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg ccatagcctg
     4981 ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga gcaggtaata
     5041 acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc gtacctgcat
     5101 gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta gtgcatcaag
     5161 ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca cctgtttatc
     5221 gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct ggaggacagc
     5281 cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga gttccagttt
     5341 ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa acaaactgtg
     5401 caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg tcggttcttc
     5461 aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat cctgagcgat
     5521 accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat cgccgggcaa
     5581 tcgttgtagc gtcctcgcat tcagttctgc agttaaagca tcattaacct gccagggctg
     5641 gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg cttcaataca
     5701 ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc cgagcgcact
     5761 ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg gtgcaacgcc
     5821 gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca gatcttccgc
     5881 cagacgttcg acctccgcaa gctcgacgct ttctggcgca gcgttaagat ccagaacagc
     5941 ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat tggtaataaa
     6001 tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga caatctgcac
     6061 cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt ctggctcgag
     6121 gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg ttccggcttc
     6181 ttgtagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc ctgccgttgc
     6241 cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg gcataacgcc
     6301 acgtttgcgc cagatttgcc gataaccatc gaactcttca actaccgcat cccacgcatg
     6361 ttcgtcatta ttcagcgttt cgatatccag cgcgttcagc cccatcattg acgttgccag
     6421 cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca accaaagcat
     6481 ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa cggaagggat
     6541 ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc gcaccagcac
     6601 actgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca acgcttcgcc
     6661 ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg ccatggtact
     6721 ttgataatcg ccaacgccgc agctttcgcc ttccatcagc cacattttca tcgcaggctg
     6781 cgtttcacct ttaaacacaa aacgtaacgc ctgatttttc ccggctgatt tcactggaat
     6841 aaacggtatt tcgcgaaaca tgaacgcgtc atcagtctgg ctgaaaagct tgttcacact
     6901 gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg cgtgaacttc
     6961 actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata tggcctgctt
     7021 cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac ggcgaaaaat
     7081 tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg ggaatcgcgt
     7141 acggatcgcc gctgccaaca cctcaccgct ttcgctacgc agcgcggaat cgagccgact
     7201 taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta ctgtttcgcg
     7261 gatctcagcc aatgcgcggg tgatcaccag atcgcggatc gacaatggtt ctgcaagcag
     7321 ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct tcgtgcgatc
     7381 ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac tgtttgtctc
     7441 ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac ggttaaactt
     7501 gcgtcgatca ataccagaag attcgatcag cgcatcaagt tcacccactg cgtcgcgcca
     7561 ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag ccagcgtttc
     7621 atcatcgggc ggcggtgctt tgataaccgg tgcttcgcct tgcagataac gattaatatc
     7681 gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacc accagggcaa tttcacgcgg
     7741 cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta gcagagactc
     7801 atcttcaatc agctgctgct caaacagcat accggattca aaggcattca ggttgagcat
     7861 gcgctggcaa aaaccgtgaa tagtaaagac tgccgcttca tccatctgcc gttcggctaa
     7921 caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc gttcgtacag
     7981 tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat tgctacggat
     8041 acgaccacgc aattctgccg tggcagcctc ggtaaaggtg accaccagca gttcttcaac
     8101 ggtcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc gcaaatagag
     8161 cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc gctcgccctg
     8221 taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt tcactcatca
     8281 ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct tcaggatgca
     8341 catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc atgccttgcg
     8401 gctcgaccac cgccagatgg aagaaatcag caagtttttg cggcgtcagc agttttatct
     8461 gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa tctttactta
     8521 acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta attaccgcct
     8581 gctggatttg cgcaaactct tctggcttca tcgcccgcaa ttttgcctct gcggttggga
     8641 aaaacgcctt gtaacgctcc cacaagaagg aaggctgttt atcattgctt tgcaaaagga
     8701 agcccatgcc ccactgacgc cccacgctca ttggaaatgc aaacacggca tagcccaatt
     8761 gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc aacagagagc
     8821 tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct gccagtgcgg
     8881 agtcggtgct gttaccagct ttttcaaaga tgacggattg ttttttatcg accacgacat
     8941 ctttgttacg acaccactct gaaccatcag cgcccaactg tttttgcaca tcgcgtgcca
     9001 gcgttgttgc ctggtcctcg gtcatgttgc cgataaccat aaactctggt cgagcccctg
     9061 attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc aaaattttgc
     9121 gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc ataatcgcct
     9181 gctcgaacgc tttgcccttt tctgcggaat ccatcatctg gttataccag gacttcgcct
     9241 gctcaagctg atcttccgtg gcggtatagc taaagtagcc ttccagcaat gcctggaaca
     9301 gctgcggcag atgctgggtg taaccattag cattaaccat aaggccgttg ttagcgttgg
     9361 tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc gccagccctg
     9421 cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg gctttcggat
     9481 tacgcaaaat caggctgaca tcggcttttg gctcgctggc aaaataacgg cttggcgcat
     9541 acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcatat ttcttgtctg
     9601 acttaatcag cgagaaatcg tcaggaatat aagggttaag ctctggcaaa gagagcgcaa
     9661 tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta tcgacctgat
     9721 acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg atataccaga
     9781 tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta gcatcgtacc
     9841 gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc atggtatctg
     9901 ccagccattc gacgtaatcc atatcacggg tgattgacgg ataacggaag tcgatatcca
     9961 gcacattcgc cagttcatcg aagtattgtt tatcgatccc tttttcacgt aacaaattga
    10021 ggtagctaaa aatagccgcc acaacctgat cgcgattcgc cagaccttta tcggttaaag
    10081 acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag ttggcgctaa
    10141 tgccctcaac taatccctgc ttttgcagcc agtcggaaag tgtacctggg ctgcgattac
    10201 caatcagata ggtaatcaat tcatcggttt tgctacggaa tttcgccgag ttgttatcaa
    10261 tacgaaactc tacgcgcaac actttacgcg gcagcgccgg gacgtaatga atgataatgc
    10321 ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc tctttgttcg
    10381 gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt ttattgctgt
    10441 aaataaccgc cttcatcaga ttggcggaat agtacttctc gtggaaatct ttcagcgcct
    10501 gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca gaaaactttg
    10561 aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc atcccgtcac
    10621 gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg gcgtatttct
    10681 tgtcgagcaa gggttctgca atagcatcag ccaggcggtc taccgcacca ggcaaggcgt
    10741 cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca ttgtgactac
    10801 cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt ttcgacccca
    10861 tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga tcttccagcg
    10921 acccaacggg caccaccagc gccgagagcg atttaactgc ctgcggatca gaaaccagca
    10981 agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct ttatcacttt
    11041 tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaag ggtgcccaaa
    11101 gggcaactaa caacaataat gctttgaacc aggtgctgcg gggcattcac ggacctcata
    11161 atcaacttaa ttttctgtcc agattcaaca cgttaacgca aatcatctgc cagaatttaa
    11221 tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac tggtgcgcat
    11281 cataaagtaa gcggatagat tgcgcaattt ttatacagca ctcatgactg attaaagcga
    11341 aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt ctctggtgtt
    11401 aattgccgcc agagcctttg ataccagata tcatcacctt cgccacgcac catcatgttg
    11461 ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc gtcatccagc
    11521 atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact ttcaggtaac
    11581 accagcaatg gcgcggacat cccttcacga tacccctcaa tcagttgtga gaggtaatgc
    11641 aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc cgtctttgcg tagaaaaagg
    11701 cgactttcac cattaccacc gctggcacag tagacaaggt gttccagcca aagttgcatt
    11761 ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg ctgcacctgc
    11821 ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat actctgcccc
    11881 ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca ctgtgtttcc
    11941 cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa gcggcggaac
    12001 aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt gatttgataa
    12061 cggctaagcc cttccagaat aaatggctcg gtgtctggga tttcgctgtc ttcagtacgg
    12121 aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca gaatcgttgt
    12181 agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac aaattcagaa
    12241 tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct ttgtcgttcg
    12301 cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt aagatgcgct
    12361 tttacccttg cctcgctttc atcacagttg agcgcttcat cgtccggtag ataatgactt
    12421 tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc actgttatcc
    12481 tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat taacgcttcc
    12541 aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg tttctggctc
    12601 atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt cattcccagc
    12661 aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat gttaaccggt
    12721 ccggcgagaa aacgctggct gatacgttct tgatccagac gctgcgccag ttcatcacgc
    12781 aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc gataatcgcc
    12841 tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg caggaagaag
    12901 gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc ctgtgccagc
    12961 ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac cagttctgca
    13021 attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg cgcgctctcc
    13081 atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg tccggtggcg
    13141 gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc ggattcgttg
    13201 acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc cgccagcacc
    13261 ggcacatcca gtaatgccag cacgtcttcc gacacaaagc ggctgtctgg caacgataac
    13321 aggctgataa acgcttccag taccggatgc gactgccgcg cccgacggtc ggaaatggcg
    13381 taaggcaggt aacgatccgc aggtgcgcta ccaaacacag cctgaataaa cggactgtag
    13441 ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg gtcttcttcc
    13501 agcatcgcca gcaggcgatc gtgcaaaact tcaacttcac gctgcgggct atggcaaacg
    13561 tggaaggtga tactgctatc cagtggatca agcgggcgtt tgttatcgct acgggaaaac
    13621 tcttcgatgt tcacaccagc aacggcacgg ttttccagtt ccagaatgtc agactgaatg
    13681 ttatgcagca ggttatctgg cgtgacatcg acaaaggcgt ccagctcctg gctgctctcc
    13741 aggtcagaaa ggagataaat gtagtcgcgc ccaagcttac cccatgaagc cagcagcggg
    13801 ttgccgacat cctgttcacc atcgctgtta aagagctgcc cggcattttc gctatcacga
    13861 aatagcggca attcgcgatc ttcaacactg tgtcgacgct gacgggtcag cagtttcgcc
    13921 agataagcag gatctttaat atcgccccag taataacggc aggggttggt aaacaggaga
    13981 tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg cggtaacgcg
    14041 gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt cgcggactcc
    14101 agcgtttcga taaagcgctg atagagattg gcgcggtgcc aacgcggttg tccgagttca
    14161 tgggtatatt ccaccagcgc cttccacaac ggcgcttgcc aggcctgtgc ttctcccagc
    14221 ccttcaacca aatgtcctgt ttcccactgt gccagccact ccgggcgata gaccagatac
    14281 tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt gtcgctatcg
    14341 tcagccagat aatgccgcaa cagggtaaag tcttcgcgat ccagcaattg cggcagcaga
    14401 gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg gatctccggt
    14461 aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc aatgtttgcc
    14521 gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc ggtactttgc
    14581 accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac aataaactcc
    14641 atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat agcggctcct
    14701 gactactgac gattcgggca atgcaggcgg gtcatctcgc cttctctgcc cccgggtgaa
    14761 actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg gttgacctgc
    14821 cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg ccgccagagc
    14881 tgttggtact ggtttctgct ggcgagactg ttcattaatg ttcgctggat acccgataac
    14941 gcagtgacaa tcatcaccat cagcaccatc gcaaccatta cctccggcag gctaaagcct
    15001 tgttgattct tcagggaagc tgacataacg ccctctcttt cagcggacaa aaatcgctcc
    15061 agccgcgtgg cgaaaaaaca atgtttccat caatgacttc gcctgttcgc cacaacgaaa
    15121 cgccttcata accggcaacc aataaggctt cattatctgc cagtaaacgc aaacaaaccc
    15181 gggcatcggt tccagcgtac tgcgagcact gaactgcggg ctgcgtctgc caggagtgca
    15241 ttttccccca cgccagcgcc gactgaacga tggcctggcg gcgcaatgac tgactttcca
    15301 tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat tccttgcaat agcaagctac
    15361 ccaaaaccag cagcatcagg accagagcca gtgacgaaac acctttttcg cggttcacag
    15421 gttgaatcct gtcacgctat agctggcatc caccacggtt tgcggttcag acttactggc
    15481 agcacgcata ttaaccgtca acaccggcga gaagccgctg acatcctgac gtacgacctg
    15541 aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct taccttcaca
    15601 ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc caatctggtc
    15661 agactctttt accggttcgc gatcccagat accgttactg ttcgcatccc actgcacaat
    15721 gatacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc catgacaata
    15781 acccgccctc tggagatgct tcgcgacggt aaatacccgc agccagattt catcttccag
    15841 cgccagctta cgggtgctcg ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc
    15901 ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca gagaaaaacc
    15961 ttgctctttt acaggcatcc ttctgtttct ccttgctgac aaagccggag tcttccccac
    16021 ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg cccggcccat
    16081 gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg
    16141 ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc cccagcagcg
    16201 gaactcacaa ggcaccataa cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta
    16261 tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctggct ggcggtttgc
    16321 catagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc gcttagcatg
    16381 accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg tgttttcatg
    16441 ccggcagtat ggagcgagga gaaaaaaaga cgagggccag tttctatttc ttcggcgcat
    16501 cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga
    16561 atttaacgga gggtaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt
    16621 aattagatag ccaccggcgc tttaatgccc ggatgtggat cgtagccttc aatctcaaag
    16681 tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc
    16741 ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca tatgattgct gtacaggtgc
    16801 gtgtcgcccc ccgtccagac aaaatcgccc acttccagat cacactgctg cgccatcata
    16861 tgcaccagta acgcgtagct ggcaatgtta aacggcaggc cgaggaaaac gtcacaggag
    16921 cgctgataaa gctggcaaga gagtttgccg tctgccacat agaactggaa gaacgcatgg
    16981 cacggtgcca gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg
    17041 cgcgaatccg ggtcgttttt cagctggttc agtaccgtag tgatctggtc aatatgacga
    17101 ccatccggcg ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccgttt
    17161 tcatcggccc attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagtg
    17221 tcgccctgca gaaaccacag cagttcatgg atgatggaac gcaggtggca acgtttagtt
    17281 gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc
    17341 gttccggttc cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt
    17401 tctaaatact gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc
    17461 cagaccatca tgatcacacc cgcgacaatc atcgggatgg aaagaatttg tcccatgctg
    17521 atgtactgca cccaggcacc agtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg
    17581 atgcgaaacg cgccgtaacc aatcaggaac aaacctgaga cagctcccat tgggcgcggt
    17641 ttacgaatat acaggttgag gataataaac agcactacac cttccagcaa cagctcgtaa
    17701 agctgtgatg ggtggcgcgg cagcacaccg taagtatcga aaatggattg ccactgcggg
    17761 ttggtttgca gcagcaaaat atcttctgta cgggagccag ggaacagcat ggcaaacggg
    17821 aagttcgggt caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca
    17881 ccaagaccaa acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta
    17941 gtacggcggg cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaggac
    18001 atgccgccgt cccagacacg gaacagatac agcggatcgg ccataaactg cgggaagttg
    18061 tagaacagaa cataaccaat acgtccaccg aggaagacgc cgaggaagcc cgcatagagt
    18121 aagttttcaa cttcattttt ggtccagcca ctgcccggac gattcgcccg tcgtgttgcc
    18181 agccacattg caaaaatgaa acccaccaga tacatcaggc cgtaccagtg aagcgccacg
    18241 ggtcctattg agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc
    18301 tgtcaccaca agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca
    18361 ggtttcaggc gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat
    18421 accgttaaaa gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg
    18481 ccacgacgct ccataaaggc tgcaacctga tggcgaactt cggtcgccag ttgcgcttcc
    18541 agactacgct gcgcaagatt ttctgcttcg gcataatcaa tgcgccgcag caggtatttt
    18601 acccgcgcta cagaacgtcc gttcatagac agatggtgat acccaagccc aatgaggatt
    18661 gccacgcaca tgggatcgcc cgccatttca ccgcacaaac ggagatcgat tccatgtatt
    18721 tccgcttccc gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcataa
    18781 atgttcgcca cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg
    18841 gtgccaacag agatgaaatc gacccgcttt gccagatgcg gcagcataaa taccattgac
    18901 ggcacttcca gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg
    18961 acttcacgtc cggcacgttc aatcaggcgg cgtgcttcat caacttcatc gaggcttgtg
    19021 accatcggca acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc
    19081 acctggatca agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg
    19141 catggattct cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc
    19201 gtacgcaagg tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc
    19261 tgttcttctt ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga
    19321 ccgatgccat caatacggct gcccagtttt tcttcatgtt ccgggcttaa accagcattg
    19381 agcatgactt ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc
    19441 gccagacggc taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc
    19501 ggctccggat cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc
    19561 agcaccgaag gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg
    19621 atcgcagcat gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg
    19681 ggcagctcag caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc
    19741 caggcgttcg gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca
    19801 cgtaaatcgc cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca
    19861 aatttttcaa tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca
    19921 aacaattcgc gacgcagccg ggtgtccgaa agtaggtgcg agtaaagatc gaaaatagcc
    19981 gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc
    20041 gcttcttcca gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc
    20101 tgatacacct gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca
    20161 ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat attgcccaaa cagggcagtc
    20221 aactgcgact gggaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct
    20281 tcactttcgt catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg
    20341 cgacgttgaa taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg
    20401 atgtatttga agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg
    20461 ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt
    20521 ttcagccccc gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc
    20581 gaacagacct cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca
    20641 ttcaggcgtg gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt
    20701 ggcgtgactt aacctctttt acgtcgataa gcagatgcgt tttgtggttt tggcgtattt
    20761 tcctgcagtg acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt
    20821 ttaaatgaca ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac
    20881 tctggtgtac tgctggtttg catattgatt tctgcatcgc cgcttaccag ctgcaagaga
    20941 aaccattttt gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc
    21001 ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt
    21061 aatcctactt cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg
    21121 attccgcctt gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc
    21181 ccctggcgat tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc
    21241 ggactacctc aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt
    21301 aaaccactct ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc
    21361 agagttataa acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga
    21421 gttgtgaaga actacggaat tactacggga aaacccggag cattctgaat aagcccgata
    21481 tagacatttt aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa
    21541 agagtaattc gtgacccagc tcacacctct catttacggg ttggcgaaag atcaaccaat
    21601 gccgtattta tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt
    21661 ttcgtcgcac gtcaaggctg taaatggaaa cagtagtgga ggtttttcac agttatccca
    21721 gctttctgtg gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa
    21781 cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa aacgcaatat aaatcagaga
    21841 attgaacaac acatatggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg
    21901 ttttttaacc aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa
    21961 ctgaagaaca gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg
    22021 cggcacttgc cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg
    22081 tgttactgga gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg
    22141 ctctgggcgt ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa
    22201 cattcgtttg tgttgccccg ttaacgggta atagcggggt gacctgggaa accagccatg
    22261 tgcgccacaa gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc
    22321 tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac
    22381 agctacgcga agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga
    22441 tcaccgctcg tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc
    22501 ttaccgaagc cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt
    22561 tgaagaagaa tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc
    22621 tttgacctgc cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta
    22681 gatgttattt gcatggataa ccgatcctaa cgcctggctt gcgctcggta cgctgacgct
    22741 gctggagatc gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct
    22801 tcccacagca caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg
    22861 tctggcgctg ctggcatcaa tcgcctgggt tacgcgcctg acaaatccgc tttttacaat
    22921 attcagtcag gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat
    22981 ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac
    23041 acgcgtttca tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatctttag
    23101 cctcgactcg gtgattacgg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc
    23161 cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga
    23221 acgccatcct tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct
    23281 gattctggaa agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt
    23341 ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc
    23401 cgtacgctct cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc
    23461 ctgctttcaa gatatataac gtcggtttat aaacagacta ttatcatagg taggccaaat
    23521 gaggataacc ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct
    23581 gtttccgggt gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc
    23641 gatggaaaac ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt
    23701 aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg
    23761 tcagcatccg gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt
    23821 tatattcctt atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc
    23881 acagttcgct ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca
    23941 gcgaagccga cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg
    24001 acgttgccga aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct
    24061 acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag
    24121 tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga
    24181 agaatatccg cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc
    24241 tttatcaggt gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct
    24301 ggacggattc tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc
    24361 aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc
    24421 gctacctgca tctggcggac aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt
    24481 acagtctgtt aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg
    24541 tcgaactgct ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg
    24601 gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg
    24661 agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggcctggccc
    24721 ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg
    24781 gcgcaaccac gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg
    24841 aagacgtatt agctgaaatt gaagcggtgc atcaggttta tacttatccg gcaccataag
    24901 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
    24961 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
    25021 tatcattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
    25081 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
    25141 cgcattgagc ggaacgacaa aaaagccccc catcacgcca atcagcatca acaaggcata
    25201 ggctggcagc agctcgtgtt gcagggaaaa aatcagtacc accacgccaa tcaaaatccc
    25261 ggctggcata cagcgtgaca cggtttccag cgtaactaac ttcgctgccg cacctgcgcc
    25321 aaccacgata ccaatcgcta ccatcgcgtt gagataggtg ggcgtagcgt tatcggtaat
    25381 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgtgacac ccgctcccca
    25441 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
    25501 ggcattcagg aaactgcggg tcatgttgat gagattccag gactgccccg gacgcgccgc
    25561 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
    25621 ggccagggcg acgaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
    25681 aatcgccgct atggtagaag cttccattaa accgttagct ttcactaact tactacccgt
    25741 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacacccac
    25801 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    25861 caggccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    25921 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    25981 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtaggg cattatcgcc
    26041 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    26101 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    26161 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcgc
    26221 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgcgcg
    26281 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    26341 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    26401 gcgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    26461 agccctgctc gtcaaaacgc acaatatcgc cagtgtcata ccagccgcgc tccatttcgc
    26521 cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg cttctccacc cgcagatagc
    26581 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccaggaaccg
    26641 acaacaggcg cgcatccatt cctggtagaa tacgccctac cgtaccgggt ttcgccgcca
    26701 tcggtacgtt gatagaaacg acaggcgcgc actcggttac accatagcct tcaaggatgc
    26761 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    26821 ccaccacata gcgtagacga tagaagtcat acgggttggc gaaacgcgcg tagtgaccga
    26881 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    26941 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtaagc agtggcgtaa
    27001 acaggcctac cgtcagccca aaggagtgaa acagcggtaa tgccgacata aagcgatcgt
    27061 tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt cgccagaatg cttttatggc
    27121 tatggacgac gccttttgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    27181 cttccggctg ctgttttacc tgcgccaggc gcggcatcag caaatgagcg aagatccata
    27241 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    27301 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    27361 tggttttgat ttcagccgcc gtaatagcac tggtcagccc ttttacccca gcagtgtagt
    27421 tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa aatcactgcc gcactgatgc
    27481 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    27541 gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    27601 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    27661 gagtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    27721 cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg catcgccacc tgcgttggtg
    27781 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    27841 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    27901 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtagtg gtgatgcgtc
    27961 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatag
    28021 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    28081 accactgttg gcttattgag gtgtaaacgg caaacactgg acgcacaggt aaaaacagtc
    28141 caagcaaaat gccatcaata aaagagacgt gattaggcgt aattagaacg cgctcgccct
    28201 tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    28261 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    28321 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    28381 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    28441 atacctctgg gatcttgatt gtggtctgca cgacgactct tcgccagcga gaaaacgcaa
    28501 aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg ttactgttac agattgatga
    28561 ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta attcatgtgc tcaacccgaa
    28621 gttgacttca cctatcaata cctctgggat caccacttta gcaacctgaa gccaaacgcc
    28681 accagcggtc aatcgcaaca gcgtttcgca aagtgtaacc aaaggtttgc aatctctttt
    28741 tttgtcttac ttatttgcgt ttggctcgca ttcccacgat gaaaacacgc caccccttga
    28801 accaacgggc gttttccgta acactgaaag aatgtaagcg tttacctact aaggtatttt
    28861 catggcgacc ataaaggatg tagcccgact ggcaggcgtt tcagtcgcca ccgtttcccg
    28921 cgtcattaat aattcaccta aagccagtga agcttcccgg ctggctgtgc atagtgcaat
    28981 ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg ctggcgcagc agaccactga
    29041 aacggtcggt ctgatcgttg gtgatgtttc cgatccgttt ttcggtgcaa tggtgaaagc
    29101 ggtcgaacag gtggcttatc acaccggtaa tttcttattg attggcaacg gttaccacaa
    29161 cgaacaaaaa gagcgtcagg ctattgagca actgatccgc catcgctgtg ctgcgttggt
    29221 cgtccatgcc aaaatgatcc cggatgccga tttagcctca ttaatgaaac aaatgcccgg
    29281 tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac cgttgtattg ctcttgacga
    29341 tcgttacggt gcctggctgg caacgcgtca tttaattcag caaggtcata cccgcattgg
    29401 ttatctgtgc tctaaccact ctatttctga cgccgaagat cgtctgcaag ggtattacga
    29461 tgcccttgct gaaagtggta ttgcggccaa tgaccggctg gtgacatttg gcgaaccaga
    29521 cgaaagcggc ggcgaacagg caatgaccga gcttttggga cgaggaagaa atttcactgc
    29581 ggtagcctgt tataacgatt caatggcggc gggtgcgatg ggcgttctca atgataatgg
    29641 tattgatgta ccgggtgaga tttcgttaat tggctttgat gatgtgctgg tgtcacgcta
    29701 tgtgcgtccg cgcctgacca ccgtgcgtta cccaatcgtg acgatggcga cccaggctgc
    29761 cgaactggct ttggcgctgg cggataatcg ccctctcccg gaaatcacta atgtctttag
    29821 tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg ctggaggcaa gtcatcatgc
    29881 aaccagcgac taaccgcagt taaagcaatt ccagcgccag taattcttcg atggtctggc
    29941 gacggcgaat caaccgcgcc tgaccattat caaacagaac ttctggtaac agcggacggc
    30001 tattgtagtt ggatgacatt gatgcgccat atgcccctgt atcatgcagt accagataat
    30061 cacccgcctt cacttccggc aaggcgcggg tttcaacatt tcccccttcc tgctgggtaa
    30121 agacatcgcc cgattcacat aatggcccgg cgacgacggt ttccaccgtt ggcgcgtgtt
    30181 ccagagaacg accatcagct gccagggcac tgatatggtg gtaactaccg tacattgccg
    30241 ggcgcatcag atcgttgaac ccggcatcaa ccagcacaaa gtggcggcta cccatttgtt
    30301 tgacgctccg cacctgagta attaacacgc cagactgcgc taccagaaag cgacccggtt
    30361 caatttccag tttcacaggg tggcccaaat ggcgagcgat ttgctcacgc gcggcattcc
    30421 acagaccata ataatgttcg gtatcaaccg cctcttcacc ctgttgataa ggaatagaaa
    30481 gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa ttcgatgacc tgacgtacca
    30541 tagcaccaca cacctgttcc agatgggcat aatcaacgcc agaaccaatg tgcatgtgaa
    30601 tgccgaccag ctgcaaatga tgacgttgta tcacgtccag tgcggcgggc agatcggtgt
    30661 accagatacc gtgcttgctg ttttcgccac cggtattggt tttttggcta tgtccgtgac
    30721 caaaccccgg gttaacgcgc agccataccc gatgccctgg cgaaacctgg cccagttggt
    30781 cgagcatatc aacagaaccc gcattcaccg gaatttgcaa ttcactgacg cgttcaagcg
    30841 tcgcctgatc gataacatct gccgtaaaaa caatatcatc ggggtgcgtt tgcggattgt
    30901 aacccgccgc caacgcacgc tctatttcgc ctaacgagac ggaatccact ttcacgccct
    30961 gctcacgcat taatcgcaaa atatgaatat tggaacaggc tttctgtgca aagcgcacca
    31021 catcaaactg tttcagcgct gcaatctgcc gacgaataat ttgcgcatcg tagacccaca
    31081 ccgggcagcc aaattcagcg ggcaaacgca gcagattttc ggcggtgaga tcggtatcgg
    31141 tgctgaacag tgaatgtggc ataacaaact ccagatgagt gcttttttat gattacgcca
    31201 catcataaaa agaataaaaa atatcgattt atgtcgagtc tatgcaaaaa tgatatggat
    31261 taccggattg cgagagagcg ctaatggccg ccgttaactt acgtcatatt gaaatttttc
    31321 atgcggtaat gaccgccgga agcctgactg aggcagcaca cctgctacac acctcacagc
    31381 caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat cgggctaaaa ttgtttgagc
    31441 gcgtacgtgg acgattacat cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac
    31501 gatcctggta cggactggat cgcattgtca gcgccgcaga aagtctgcgc gagtttcgcc
    31561 agggagaact gtctattgcc tgcctgccgg tcttttcgca atctttttta ccgcagctcc
    31621 tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa tatcgtgccc caggaatcac
    31681 cgctacttga agagtggctc tcggcccagc gtcatgattt aggactcact gagacactcc
    31741 atacgcctgc gggaacagaa cgtaccgaat tactctcttt agatgaagtg tgtgtgttac
    31801 ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc ggatgatttt cagggtgaga
    31861 actacatcag cctttcccgt actgacagct atcgccagtt gctggatcag ctatttactg
    31921 aacatcaggt taaacgacgc atgatcgtag aaacccacag cgccgcgtca gtctgcgcaa
    31981 tggtacgggc gggggtaggt gtttcggtgg ttaacccgct caccgcactg gattatgcgg
    32041 caagcggttt agtggtacgg cggttcagta ttgcggttcc gttcaccgtc agcctgatcc
    32101 gccccctgca ccgcccgtca tcagcgctgg ttcaggcgtt tagtgagcat ttacaagcgg
    32161 ggttaccgaa actggtcact tctcttgacg cgattttgtc gtcagctacg acagcataaa
    32221 agcgacagca tcctcggcat ggatcgccgc agtatcaaac acaggcaaaa cactgcgctc
    32281 ttctggaacc aataaaccaa tttctgtgca gccaaaaatg acaccctgcg ccccctgttc
    32341 tgcaaggcga gcaatcactt gcgcataata agcgcgtgac gcttcggtaa attgccccag
    32401 acacagttct tcaaaaataa tctgattaat tttcgcccgt tcatccgctt caggaataag
    32461 acaattgatg gaaaattgtt ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta
    32521 acgcgtaccc agcagcgcca cacgagtcat tccggccccg gtaattgcac gtccggtggc
    32581 atccgcaatg tgtaagaaag gcagagtgca acgtgactca atggcatccg ccactttatg
    32641 catcgtattg gtacataaca caataccttc tgcgcccgcc cgctgtaagc caagcgccgc
    32701 ctcagccaga atgtccccgg ttttatccca ttccccgcga cgctggcact cttctatttc
    32761 atgaaaatcg acgctatgta gcagcacttg cgcagagtga agcccaccaa gccgctgttt
    32821 aatgccttca tttatcagac gatagtaagg aatggtggat tcccagctca ttcctcccag
    32881 caaaccaatt gttttcattc ctctctcctg atgtgtgtta ccgcaacaag gctgaataag
    32941 gaacgagaca aacgcctcaa cggccaggtg cccgatctct attaacgaaa aaagggccgg
    33001 atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc
    33061 atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat gagccagaaa
    33121 gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca
    33181 atgctatcaa gcagtgtcag gaaggtcgcg ccgataatca tattcgacac ccagtttgtc
    33241 gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc agagcacagg
    33301 atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat cgtcatgcca
    33361 acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat caggcaatag
    33421 cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc cggtttacga
    33481 cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga acataaaggt cagtcccacg
    33541 accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc
    33601 ggcgcatagt acatgatgat gttcataccg gtaaactgct gcatcgcctg caacaacata
    33661 ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca accgccttgt
    33721 tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc cgacgtatcg
    33781 cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc cagccagcgc
    33841 gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa agcaagaacc
    33901 cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa aaacgccagc
    33961 acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg aacgttttca
    34021 cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac agcaatgccc
    34081 agcaccacac gagcggcgat taacatctct acgctggtcg caaaagcgga ccctatagaa
    34141 ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgacccag gcggaacgac
    34201 agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact aaccacccat
    34261 tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc tccggcgatt
    34321 acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga aacaaacata
    34381 ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt attgatagta
    34441 accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga acaactcacg
    34501 caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat gtcgttatgt
    34561 gatggatatt cca
//
LOCUS       DAZKGS010000031        34016 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKGS010000031 DAZKGS010000000
VERSION     DAZKGS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..860
                     /gene="yccA"
                     /locus_tag="ACHOQY_001532"
     CDS             201..860
                     /gene="yccA"
                     /locus_tag="ACHOQY_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9677661.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            951..1280
                     /gene="tusE"
                     /locus_tag="ACHOQY_001533"
     CDS             951..1280
                     /gene="tusE"
                     /locus_tag="ACHOQY_001533"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9677662.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(1277..1555)
                     /gene="yccX"
                     /locus_tag="ACHOQY_001534"
     CDS             complement(1277..1555)
                     /gene="yccX"
                     /locus_tag="ACHOQY_001534"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9677663.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            1650..2840
                     /gene="rlmI"
                     /locus_tag="ACHOQY_001535"
     CDS             1650..2840
                     /gene="rlmI"
                     /locus_tag="ACHOQY_001535"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9677664.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2898..3215
                     /gene="hspQ"
                     /locus_tag="ACHOQY_001536"
     CDS             2898..3215
                     /gene="hspQ"
                     /locus_tag="ACHOQY_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9677665.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(3260..3673)
                     /locus_tag="ACHOQY_001537"
     CDS             complement(3260..3673)
                     /locus_tag="ACHOQY_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9677666.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            3846..4508
                     /locus_tag="ACHOQY_001538"
     CDS             3846..4508
                     /locus_tag="ACHOQY_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9677667.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            4604..5062
                     /gene="mgsA"
                     /locus_tag="ACHOQY_001539"
     CDS             4604..5062
                     /gene="mgsA"
                     /locus_tag="ACHOQY_001539"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9677668.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5094..7148)
                     /gene="helD"
                     /locus_tag="ACHOQY_001540"
     CDS             complement(5094..7148)
                     /gene="helD"
                     /locus_tag="ACHOQY_001540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9677669.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            7271..7717
                     /locus_tag="ACHOQY_001541"
     CDS             7271..7717
                     /locus_tag="ACHOQY_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9677670.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            7727..9889
                     /gene="yccS"
                     /locus_tag="ACHOQY_001542"
     CDS             7727..9889
                     /gene="yccS"
                     /locus_tag="ACHOQY_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9677671.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(9852..10481)
                     /gene="sxy"
                     /locus_tag="ACHOQY_001543"
     CDS             complement(9852..10481)
                     /gene="sxy"
                     /locus_tag="ACHOQY_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9677672.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            10700..11209
                     /gene="sulA"
                     /locus_tag="ACHOQY_001544"
     CDS             10700..11209
                     /gene="sulA"
                     /locus_tag="ACHOQY_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9677673.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            11566..12606
                     /gene="ompA"
                     /locus_tag="ACHOQY_001545"
     CDS             11566..12606
                     /gene="ompA"
                     /locus_tag="ACHOQY_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9677674.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(12681..13133)
                     /gene="matP"
                     /locus_tag="ACHOQY_001546"
     CDS             complement(12681..13133)
                     /gene="matP"
                     /locus_tag="ACHOQY_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9677675.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            13319..15079
                     /locus_tag="ACHOQY_001547"
     CDS             13319..15079
                     /locus_tag="ACHOQY_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9677676.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            15148..15666
                     /gene="fabA"
                     /locus_tag="ACHOQY_001548"
     CDS             15148..15666
                     /gene="fabA"
                     /locus_tag="ACHOQY_001548"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9677677.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(15736..15903)
                     /gene="rmf"
                     /locus_tag="ACHOQY_001549"
     CDS             complement(15736..15903)
                     /gene="rmf"
                     /locus_tag="ACHOQY_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9677678.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16159..16722)
                     /gene="pqiC"
                     /locus_tag="ACHOQY_001550"
     CDS             complement(16159..16722)
                     /gene="pqiC"
                     /locus_tag="ACHOQY_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9677679.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            complement(16719..18359)
                     /gene="pqiB"
                     /locus_tag="ACHOQY_001551"
     CDS             complement(16719..18359)
                     /gene="pqiB"
                     /locus_tag="ACHOQY_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9677680.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(18364..19617)
                     /gene="pqiA"
                     /locus_tag="ACHOQY_001552"
     CDS             complement(18364..19617)
                     /gene="pqiA"
                     /locus_tag="ACHOQY_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9677681.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(19747..21654)
                     /locus_tag="ACHOQY_001553"
     CDS             complement(19747..21654)
                     /locus_tag="ACHOQY_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9677682.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(21666..23774)
                     /gene="rlmKL"
                     /locus_tag="ACHOQY_001554"
     CDS             complement(21666..23774)
                     /gene="rlmKL"
                     /locus_tag="ACHOQY_001554"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9677683.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            24018..25127
                     /gene="ycbX"
                     /locus_tag="ACHOQY_001555"
     CDS             24018..25127
                     /gene="ycbX"
                     /locus_tag="ACHOQY_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9677684.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(25124..25666)
                     /gene="zapC"
                     /locus_tag="ACHOQY_001556"
     CDS             complement(25124..25666)
                     /gene="zapC"
                     /locus_tag="ACHOQY_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9677685.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(25840..26850)
                     /gene="pyrD"
                     /locus_tag="ACHOQY_001557"
     CDS             complement(25840..26850)
                     /gene="pyrD"
                     /locus_tag="ACHOQY_001557"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9677686.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(26961..27698)
                     /locus_tag="ACHOQY_001558"
     CDS             complement(26961..27698)
                     /locus_tag="ACHOQY_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9677687.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(27664..28179)
                     /locus_tag="ACHOQY_001559"
     CDS             complement(27664..28179)
                     /locus_tag="ACHOQY_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677688.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(28187..28729)
                     /locus_tag="ACHOQY_001560"
     CDS             complement(28187..28729)
                     /locus_tag="ACHOQY_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677689.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(28741..29811)
                     /locus_tag="ACHOQY_001561"
     CDS             complement(28741..29811)
                     /locus_tag="ACHOQY_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677690.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(29802..32402)
                     /locus_tag="ACHOQY_001562"
     CDS             complement(29802..32402)
                     /locus_tag="ACHOQY_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9677691.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(32427..33128)
                     /locus_tag="ACHOQY_001563"
     CDS             complement(32427..33128)
                     /locus_tag="ACHOQY_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9677692.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(33211..33753)
                     /locus_tag="ACHOQY_001564"
     CDS             complement(33211..33753)
                     /locus_tag="ACHOQY_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9677693.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         8264 a         8864 c         8081 g         8807 t
ORIGIN      
        1 ataaggggtt agctaaatgc taaccccttt ttcttttgcc tgtcgaaatt ctcagggcgt
       61 tatatttgct taatgacctg ataatccgct gttaaacctg ttccagatca aatgcgtaaa
      121 gatgggtaaa acttctgggt gcccttacgc attatcatta tgctgcttaa ttaattacat
      181 ctgtcataga gagtgactca atggatcgta ttgttagttc ttcacatgac cgtacatcac
      241 tgcttagcac ccataaggtg ctgcgtaata cctattttct gctgagcctg acgctggcct
      301 tttcggcaat taccgcaact gccagtacgg tgctgatgct gccatctccg ggtctgattc
      361 tgacgctggt gggtatgtat ggtttgatgt tcctgaccta taaaacggcg aataagccga
      421 ccgggattat ctccgcattc gcctttaccg gttttctggg ttatatcctc gggcctattc
      481 tgaacaccta tctgtctgcc ggaatgggtg acgtaatcgc tatggcactg ggcggaacgg
      541 cgttagtgtt cttctgctgc tctgcatatg tgctgaccac ccgcaaagat atgtcgttcc
      601 tcggcggtat gctgatggcg ggtattgtgg tggtgctgat tggtatggtt gctaatatct
      661 tcctgcagct gcctgctctg catctggcga tcagcgcggt cttcattttg atctcctctg
      721 gcgctatttt gtttgaaacc agcaacatcg ttcatggcgg tgaaacgaac tatattcgtg
      781 ccacggttag cctgtatgtt tcgctgtaca acatcttcgt cagcctgctg agcattctgg
      841 gcttcgccag ccgcgattaa tctcacccgc taacactttc atagcctcgc tttatgcggg
      901 gctttgtttt ttgttacact gccgccagtc tgattaaggt agtaataatt atgctgatct
      961 tcgaaggtaa agagatagaa acggataccg aaggctatct caaagaaagc agccagtgga
     1021 gtgagccgct ggcagtggtg attgcagaga acgaagggat ttcgctgtca ccggaacact
     1081 gggaagtggt gcgttttgtg cgtgatttct atctggaatt caatacttct ccggcgattc
     1141 gtatgctggt aaaagcgatg gcgaataaat ttggcgaaga aaaaggtaac agccgctatt
     1201 tataccgact gtttccgaaa ggtccggcta agcaagccac caaaattgct ggcctgccaa
     1261 aaccggtaaa atgtatttaa cggatacgaa aatcggttaa ttcccccgag ggatgatgcg
     1321 gctcgctgag cacccgttct acacgcgcag aacgcgggcc gccactcttt aaccacagca
     1381 ttaatttttc cacctgccct tcttcaccgc aggcaaccac ttccacgctg ccatcgtcaa
     1441 gatttttggc gtacccggtt aaacccagtc ttttcgcttc gtactgtgtg gtgtagcgaa
     1501 atcctacgcc ctgaactcgc ccgtaaaccc aggcaattat gcagactttc gacattgctg
     1561 ctctccttat ccttaatgga cgcgttgcaa atccatggaa aactccggac aatgacgccc
     1621 atttctttgc atcgacagaa aagtaaatta tgagtgtacg tttagtgtta gccaaagggc
     1681 gcgaaaaatc attacttcgt cgccatccgt gggtcttttc cggggccgtt gcccgcatgg
     1741 aaggtaaagc cagcctcggt gaaaccatcg atattgttga tcatcaggga aaatggttag
     1801 cacgcggcgc ttattcacca gcttcgcaaa tccgggcgcg cgtctggacg tttgacccgt
     1861 ctgagtctat cgacattgct tttttttccc gccgtttgca acaagcacaa aaatggcgtg
     1921 actggctggc gcaaaaagat ggcctcgaca gctatcgttt aatcgccgga gaatctgatg
     1981 gcctgccggg tattactatc gatcgtttcg gtaattttct ggtgctgcaa ctgctgagtg
     2041 ctggggcaga atatcagcgc gcggcattag ttagtgccct gcaaacgctg tacccggaat
     2101 gtgcgattta cgatcgcagc gatgttgcgg tacgtaaaaa agaagggatg gagctgaccc
     2161 agggcctcgt caccggcgag ttgccgcctg ccctgctgcc gattgaagaa cacggcatga
     2221 agctgctggt ggatattcag cacggacaca aaacgggcta ctacctggac cagcgtgata
     2281 gccgcctggc tacccgccgc tacgttgaaa ataaacgtgt gctgaactgt ttctcctata
     2341 ccggtggttt cgccgtatcg gcactgatgg gcggttgcag ccaggttgtc agcgttgata
     2401 cctcccagga agcgctggat attgcacggc agaacgttga gctgaacaaa ctggatctga
     2461 gcaaggctga gtttgtccgt gatgatgtct ttaaattgct gcgtacttat cgcgatcgcg
     2521 gtgaaatatt tgacgttatc gtgatggacc cgccgaagtt tgttgagaat aaaagccagt
     2581 tgatgggcgc gtgtcgtggc tataaagata tcaacatgct ggcgattcag ttgctgaatg
     2641 aaggcggtat tctcctgact ttctcctgtt ccggtctgat gaccagcgat ttatttcaga
     2701 aaatcatcgc ggatgccgca attgatgccg gccgtgatgt acaatttata gagcagttcc
     2761 gtcaggcagc cgatcatccg gtgatcgcta cctatccgga agggctatat ctgaaagggt
     2821 ttgcctgtcg cgtcatgtaa cttgaaatgt ggaattttac ccttatatca aaggtaaaga
     2881 tttcccggga ggtgactatg attgccagca aattcggtat cggccagcag gtccgccatt
     2941 ccctgttagg ttatctcgga gtggtcgtgg atatcgaccc ggtttattcg ctctctgaac
     3001 cgtcgcctga tgagctggcg gtgaatgacg agcttcgcgc cgctccgtgg tatcacgtgg
     3061 tgatggagga cgataacggc ctaccggttc atacctacct ggctgaagcc cagctaagta
     3121 gcgaattgca ggatgaacat cctgaacagc catccatgga tgagctggcg cagaccatcc
     3181 gcaaacaact ccaggccccg cgtctgcgta actgatccct gaacgaatcc ccggaaggtt
     3241 tttgccttcc ggggattttt tatttggcca ggcccaggcg agggatctca atagccgggc
     3301 agcgatccat caccacattc aacccagcat cccgtgccag tactgccgct tgctcattaa
     3361 tcacccccag ttgcatccag agggttttcg cgccaatggc aatagcttcc tgtgccacgc
     3421 cccacgccgc ttctgaatta cggaaaacat cgaccatatc gaccttttcc ggaacgtctg
     3481 ccagcgtgcc atacccctgc tggcccagca gcgttttgcc ggcaactttt ggcgaaaccg
     3541 gaatgacgtg atacccctgg tcgagtagat atttcatcac gcgatagctg ggacgatcgg
     3601 gtttgtcgct tgcccccacc agcgcaatgg tgtgagtgga cgttaaaatg cctgcaatat
     3661 cggtttcttt cattatttcc tccagactgt ttaacaaagt gtacgacaaa gccgacatgc
     3721 caaccatgca gctaatacga atgaatgaga gcaaaagcgg gaaatatgtc tatatgttag
     3781 taagtatgat taaagaaaaa ttttgacaca ttattattcg ggcggtcttt tgacaggagc
     3841 tacctatgaa aaccggcatc gtgaccacct tgattgcatt atgtttgccg gtatccgttt
     3901 ttgccaccac gctgcggctt tcaaccgatg tcgatcttct ggtactcgac ggcaagaagg
     3961 tttccagctc tctgctgcgc ggtgcggaca gcattgaact ggataatgga ccgcatcagt
     4021 tagtgtttcg cgttgagaag acgattcatc tttccaatag tgaagaacgg ctgtatatct
     4081 ccccaccgct ggtggtcagt tttaataccc agctcatcaa ccaggtcaat tttcgcctgc
     4141 ctcgcctgga gaatgagcgg gaagctaacc attttgatgc cgcgccgcgc cttgaattgt
     4201 tggatggcga tgcgacgccg attccggtaa agctggatat tctcgccatt acctcaacgg
     4261 caaaaacgat tgattatgag gtcgaggttg aacgctataa caaatccgcg aaacgcgctt
     4321 cactaccgca atttgccacg atgatggcag atgacagtac gctgctttcg ggtgtttccg
     4381 agctggatgc tattccaccg cagtctcagg tgctcacaga acaacggctg aagtattggt
     4441 ttaaactggc tgacccacaa acgcgaaata ctttcctgca gtgggcggaa aaacaaccat
     4501 cttcctgaga tttttgtgcc tgtgcgcagg ctttttcggt ctttatcttg cagcgataag
     4561 tgcttacagt aatctgtagg aaagttaact acggatgtac attatggaac tgacgactcg
     4621 cactttacct gcgcggaaac atattgcgct ggtggcacac gatcactgca aacaaatgct
     4681 gatgagctgg gtggaacggc atcaaccgtt actggaacaa cacgtactgt atgcaacagg
     4741 cactaccggt aacttgattt cccgtgcaac cggcatgaac gtcaacgcga tgttgagtgg
     4801 cccaatgggg ggtgaccagc aggttggcgc attgatctca gaagggaaaa ttgatgtatt
     4861 gattttcttc tgggatccac taaatgccgt gccgcacgat cctgacgtga aagccttgct
     4921 gcgtctggcg acggtatgga acattccggt cgccaccaac gtggcaacgg cagacttcat
     4981 tatccagtcg ccgcatttca acgacgcggt cgatattctg atccccgatt atcagcgtta
     5041 tctcgcggac cgtctgaagt aatattgcac aggtggcaaa cgccacctgt ttcttacggt
     5101 tttctcgcca ccggcacatc cagatttttc agtatttcca caaagggaga gggattctct
     5161 ttgttaaaca gtgcccatac ccgatggcgt gcccgggtca gcgccacgta cattaaccgc
     5221 cgttcttcag cgtccgggaa atcctcaacc ggtggcagta gcgcctcttc cataatcgac
     5281 tcccgcgccg cagccggaaa accatcactc ccctcctgca agccaacgat gatgacgtaa
     5341 tccgcctgtt gccctttgct ggcatgaatg gtcataaagt cgatttgcaa cttcggccag
     5401 cgtgttgccg ctttttccag gctggcaggc ctcatgtgat ggtaacgcgc caggataaga
     5461 atgcgctctt ccggtttggc ataaccagag agcttatcca gcaaagcgtc aagttgactc
     5521 tcatccaata acgtgacggc tttttcgtct ccattggtta agctgtttag cggctttttc
     5581 aactggcctg ggttctgctg aataaaccgg tttgccacct caccgatacg actgttaaaa
     5641 cggtaagtcg tgtctaaatc acagcgatcg ccttcaccaa agttttcatg gaaagcggtg
     5701 gtgagcgaca tttgcgcgcc gctgaatcgg taaatcgcct gccagtcatc accaacggcg
     5761 aacaacgtcg tctgactgtt ttgcttgcgt aatgccgcta acaacgctgc ccgctgcggc
     5821 gagatatcct gaaattcatc aaccagaata tgcttccacg ggctgataaa gcgacctttc
     5881 tccagaatca caatcgcctg atgaataagg cccgaaaaat cgacagcgtt ttctgccttc
     5941 agcgcacctt tccaggcttt taataacggg gccatcaact tgatacgttt actgaacaga
     6001 tcgcgaatct cttcgggtgc actggcaatc atttctgcct gtgcaccacc gtgcatccgc
     6061 atcagactta cccagcgatc gaggcgagag gcaaggcgac gctgtaattt ttcatcatcc
     6121 cagaagttac cttctggcac tgaccactgc atttcttccg tcagccattg ccgccagccc
     6181 ttcgcctgcg ctttcttttc gctgcattgc ttgcgccact cagcaataaa gagttcatga
     6241 cgggcagcgg tatcattttc cagtttgctg actatcggaa cttttttgct gccctgctga
     6301 ataatatgca gcgccagcgc atgaaacgtg cgtgcggtaa tgtcttcggt atgtagccgt
     6361 tcgcgaatcc gctcgtccat ctcttcagcg gctttgcgac caaacgccag caataaaatt
     6421 tgctcagggg acgcttcacc acgcgccagc aaccagcctg cacgggccac cagcaccgac
     6481 gtttttccgc ttcctgcacc tgccagcact aacagagaat gctcgccatt aacgactgcc
     6541 cgggcctgcg ccggattcag cggtgaagac tcgacctggc ggaaaaaatc cgcatactcg
     6601 gtaagcatgg cttcggtata cgcctggtta tgctgcaacc gagcgctttc aatatctttc
     6661 aaccaggcct gacatttacg ccacgcctca cggcagttat cgaattcttc cagtcggtta
     6721 accggcaacg gcaacgccga caaagcctgg cggatttgtt gctgcacgcc agaggtttgc
     6781 tcacgcgtca gccatttatt ttccccagtg cgcgtggcaa tcaaatccag ttgctggcgt
     6841 aaaacaccag acgcaatttc gctcatctcg ccactccacc gccgccagtg agcatcaaga
     6901 tgatggtaaa aacgctgcgt ctcgccccat tcggtgccgt gcagacgcac cactttttcg
     6961 tccggcagta caaattccag ctcgccccat accagaccgc gcttacagtg aatcgccagt
     7021 aattgattga aaggaataag gtattcgtgg cgatcgccgg agactttaat cccggcattg
     7081 aggatcaccg cccgatcgta agggtgctgt gccagacgtt ttccaagcgt tgtcgctttc
     7141 agttccatga ctctgtagat ccacacgaaa ttatttctta tcagtgtaac cgtcagagaa
     7201 aacgtgctcc agcccaaaaa aacgttacaa ttgccgccca ttactgaaaa ccacagtaaa
     7261 gcgaggtttt atgcgtaccg ttttgaacat tctgaacttt gtgcttggcg gatttgccac
     7321 cactctgggc tggctgttgg cgactctggt cagtattgtg ctgattttta ccttaccgct
     7381 gacacgatcc tgctgggaga tcactaaact gtctctggtg ccttatggca atgaagctat
     7441 tcatgtcgat gaactgaacc cggctggcaa aaatgtactg ctgaatactg gcggtacggt
     7501 attgaatatt ttctggctga ttttctttgg ctggtggtta tgcctgatgc acattgcaac
     7561 gggcatcgca caatgtattt caatcattgg cattcctgtc ggcattgcga actttaaaat
     7621 tgccgctatt gcactatggc cggttggtcg tcgcgtggta tcggtagaaa cagcgcaagc
     7681 tgcgcgtgaa gccaatgcac gtcgtcgttt tgaataatcc actaatatgg cctttatgct
     7741 aagtcctttg ctcaaacgct atacctggaa cagcgcctgg ctgtattacg cgcgtatttt
     7801 tattgcgctt tgtggaacca cagcgtttcc gtggtggctg ggtgatgtaa aactgacgat
     7861 tccgctaacg ctggggatgg tggcagcggc gctgaccgat ctcgatgacc gactggcggg
     7921 acgtttgcgt aacctcatca ttacgctgtt ctgctttttt atcgcctcgg cctcagtaga
     7981 attgctgttt ccctggccct ggctatttgc gattggctta acgctctcta ccagcggctt
     8041 cattttgctc ggcggtctgg gtcaacgcta tgcaacaatt gccttcggtg cattgctgat
     8101 cgccatttac actatgttgg gaacatcact gtatgagcac tggtatcagc agccgatgta
     8161 tctgctggcc ggtgccgtct ggtacaacgt cctgacactt attggtcatc tgctgttccc
     8221 ggtccgcccg ctgcaggaca acctggcgcg ttgctatgaa caactggcgc gttatcttga
     8281 gctcaagtcg cgcatgtttg atcctgatat tgaagatgaa agccaggcac cgctgtacga
     8341 tttggctctc gccaacggtc tgctgatggc gacattgaat cagacgaaac tctcgctgct
     8401 gacccgctta cgtggcgatc gtggtcaacg gggaacgcgt cgcacgctgc attattactt
     8461 tgtcgcacag gatattcacg agcgtgccag ctcttctcat attcagtatc aaacattgcg
     8521 tgaacatttt cgccacagcg acgtgctgtt ccgttttcag cggctgatgt cgatgcaggg
     8581 ccaggcgtgc cagcaactgt cacgctgtat tttgttgcgt cagccttatc aacatgatcc
     8641 gcattttgag cgcgctttta cgcatattga tgctgcgctg gagcggatgc gcgataacgg
     8701 cgcacccgcc gatttactca aaacactggg atttttgctg aacaatttac gcgccattga
     8761 tgcccaactg gcaacaattg aatcagaaca ggcccaggca ctaccccata ataatgacga
     8821 aaatgagctc gctgatgaca gcccgcacgg gttgagtgat atctggctgc gtcttagccg
     8881 tcacttcacg ccggaatccg ccctcttccg tcatgcggta agaatgtcgc tggtgttgtg
     8941 cttcggctac gccatcattc aaataaccgg aatgcatcac gggtattgga tcttgctgac
     9001 aagtttgttt gtctgccagc caaactataa cgccacgcgc caccgcctga agttaaggat
     9061 tattggtacg ctggtaggta tcgccattgg cattcctgtg ctgtggtttg tgccatcact
     9121 ggaagggcag ctggtgctgc tggttattac cggcgtgctc ttttttgcct tccgtaacgt
     9181 gcaatacgct catgcaacga tgttcatcac acttttggtg ctactgtgtt ttaacttact
     9241 gggtgaaggt tttgaagtag cgttacctcg cgtaatcgat acgctgattg gttgtgccat
     9301 tgcgtgggcg gcagtgagct acatctggcc tgactggcag tttcgcaatc tgccgcgcat
     9361 gctcgaacgc gccacagagg ccaactgtcg gtatctcgat gccatactgg agcaatacca
     9421 tcaggggcgt gataaccgtc tggcgtatcg tattgcccgc cgcgatgcac acaaccgtga
     9481 tgctgagctg gcgtcggtgg tatcaaatat gtccagcgag ccgaacgtta ccccgcaaat
     9541 tcgcgaagcc gcgtttcggt tgctgtgcct taaccatacg tttaccagct atatctcagc
     9601 cctcggtgct caccgggagc agttaactaa tcctgaaatt ctggcgtttc ttgatgacgc
     9661 agtttgctat gttgatgacg cgttacatca tcaacctgct gatgaagaac gcgtcaatga
     9721 ggcattagct agcctgaaac agcggatgca gcaacttgaa ccacgggcag acagcaaaga
     9781 acctctggtc gtacaacaag ttggattatt gattgcatta ctgcctgaga ttggtcgtct
     9841 gcaacgccag attactcaag ttccgcagga aactcctgtt tcggcgtaag agagtcagcc
     9901 cattctgcaa gctcctggcg gcgtgccacc gggagcgcag cttcatgaat gccgataatg
     9961 gcaccttcaa gcataaacag aatcttttct gtcaccagac tgttttgctg ccgcagtcgc
    10021 aaccaacaca tttttgcccc aagtatacgt aacgcccgta catccttaat ccccacctcc
    10081 ccgagaatcg cttccagatg aaaagacata ttgggcaaat ctttcagtct ttcccgggta
    10141 ttgcgcgtgc ttttctcttt cagcgctgca tcgagagaat atttcgacag acgcaccagc
    10201 ttcagttgat ttcgccatag actttcatca acccgatagt aattgagggt aacggatcgg
    10261 ccacactttt tatatgtcag ccagacaggc ggatgtttta cacagtactg tgcactttgc
    10321 tcacaagccc gaagatacaa ctcaccatca gaaaccatcg caaacaccgt gtcgtcaacg
    10381 gtcaggctgt aactgccaaa caatgatcgg tattcaattg tgcccaacgt tgccaggtat
    10441 tcttgtgatt tatagatccg cttataggag aggcttttca taaaattcct tttaaaatca
    10501 taacataaaa gaatgattca cattaacgga tccgttaact acgaaaatag gcaacttatt
    10561 cttaaggggc aagattaatt tatgttttcc cgtcaccaac gacaaaattt gcgaggctct
    10621 ttccgaaaat agggttgatc tttgttgtca ctggatgtac tgtacatcca tacagtaact
    10681 cacaggggct ggattgatta tgtacacttc aggctatgca catcgttctt cgtcgttctc
    10741 atccgcagca agtaaaattg cgcgtgtctc tacggaaaac actacagccg ggcttatcag
    10801 tgaagttgtc tatcgcgaag atcagcccat gatgacgcaa cttctactgt tgccattgtt
    10861 acagcaactc ggtcagcaat cgcgctggca actctggtta acaccgcaac aaaaactgag
    10921 tcgggaatgg gttcaggcat ctgggctacc cttaacgaaa gtaatgcaga ttagccagct
    10981 ctccccttgc cacactgtgg agtcaatggt tcgcgcttta cgcacgggca attacagtgt
    11041 ggtgatcggt tggttggcag atgatttgac tgaagaagag catgctgaac ttgttgatgc
    11101 ggcaaatgaa ggtaacgcta tggggtttat tatgcgtccg gtaagcgcat cctctcacgc
    11161 cacgagacaa ctttccgggc taaaaattca ctctaatttg tatcattaag taaatttagg
    11221 attaatcctg gaactttttt tgtcgcccag ccaatgcttt cagtcgtgac taattttcct
    11281 tgcggaggct tgtctgaagc ggtttccgcg attttcttct gtaaattgtc gctgacaaaa
    11341 aagattaaac ataccttata caagactttt ttttcatatg cctgacggag ttcacacttg
    11401 taagttttca actacgttgt agactttaca tcgccagggg tgctcggcat aagccgaaga
    11461 tatcggtaga gttaatattg agcagatccc ccggtgaagg atttaaccgt gttatctcgt
    11521 tggagatatt catggcgtat tttggatgat aacgaggcgc aaaaaatgaa aaagacagct
    11581 atcgcgattg cagtggcact ggctggtttc gctaccgtag cgcaggccgc tccgaaagat
    11641 aacacctggt acactggtgc taaactgggc tggtcccagt accatgacac tggtttcatc
    11701 aacaacaatg gcccgaccca tgaaaaccaa ctgggcgctg gtgcttttgg tggttaccag
    11761 gttaacccgt atgttggctt tgaaatgggt tacgactggt taggtcgtat gccgtacaaa
    11821 ggcagcgttg aaaacggtgc atacaaagct cagggcgttc aactgaccgc taaactgggt
    11881 tacccaatca ctgacgacct ggacatctac actcgtctgg gtggtatggt atggcgtgca
    11941 gacactaaat ccaacgttta tggtaaaaac cacgacaccg gcgtttctcc ggtcttcgct
    12001 ggcggtgttg agtacgcgat cactcctgaa atcgctaccc gtctggaata ccagtggacc
    12061 aacaacatcg gtgacgcaca caccatcggc actcgtccgg acaacggcat gctgagcctg
    12121 ggtgtttcct accgtttcgg tcagggcgaa gcagctccag tagttgctcc ggctccagct
    12181 ccggcaccgg aagtacagac caagcacttc actctgaagt ctgacgttct gttcaacttc
    12241 aacaaagcaa ccctgaaacc ggaaggtcag gctgctctgg atcagctgta cagccagctg
    12301 agcaacctgg atccgaaaga cggttccgta gttgttctgg gttacaccga ccgcatcggt
    12361 tctgacgctt acaaccaggg tctgtccgag cgccgtgctc agtctgttgt tgattacctg
    12421 atctccaaag gtatcccggc agacaagatc tccgcacgtg gtatgggcga atccaacccg
    12481 gttactggca acacctgtga caacgtgaaa cagcgtgctg cactgatcga ctgcctggct
    12541 ccggatcgtc gcgtagagat cgaagttaaa ggtatcaaag acgttgtaac tcagccgcaa
    12601 gcttaagttc tcgtctggta gaaaaacccc gctgctgcgg ggtttttttg cctttagtaa
    12661 attgaactga ctttcgtcag ttattcctta cccagcaatg cctgcagatc ctgcttcaga
    12721 gaagacattt tattcgcgta tttctctttg ttttccgcat cttcaatcag ctgaacaatc
    12781 gtttcagaaa gcgttttacc gcgacgctgc gcaagaccag ccagacgttg ccagacaata
    12841 aattccagat cgatcgattt tttgcgcgta tgctggtgtt ccgcattaaa atggcgcttt
    12901 cgtcttgccc gaatggtttg cttcatgcga ttgaccagtt ccggattcat atgcttgtca
    12961 atccagccat ttaccagcac gggttcattt tcgagcgaga gcaacacatc gacggcttcc
    13021 tgggcggcac tggcttctat gtaacgggtg attaactccc cttcgcgatg cttcttcacc
    13081 agatacttcc atttccaacc gctttcaaga ttttcaagtt gttgatattt catagcgatc
    13141 tcaatgttac cgtgtaactc tttacagaat atcagctttt taggccagtg aagaaaagaa
    13201 tctccatcct gtgagcttta acgcccatgc caggagtatt gtcggtgctt tctacgtgtg
    13261 ctgtagtgcc ggttacggta taatcgcggc tttgacaaca gactaaaaaa catcaacttt
    13321 gaccattacg aaacttgcat ggcgtgacct ggttcctgat accgatagct atcaggaaat
    13381 atttgctcag ccacatttga ttgacgaaaa cgatccttta ttcagtgata ctcaaccgcg
    13441 actgcaattc gcactggagc agttgctgca tacgcgagca tcctcctctt ttatgctggc
    13501 gaaggccccg gaagagtctg agtatctgaa tcttattgcc aatgccgcgc gtacgctaca
    13561 aagcgatgca ggccaactgg tgggcggtca ctatgaggtt tccggccact ccatccgctt
    13621 acgtcacgca gtgagtgcag atgataattt tgcgacttta acgcaagttg tcgctgccga
    13681 ctgggtagaa gcggagcaac tctttggctg cctgcgccag tttaatggcg acattaccct
    13741 gcagcctggt ctggtgcatc aggcaaatgg cggtattctc atcatctctt tgcgtacact
    13801 gctggcgcaa cctctgctgt ggatgcggct gaaaaatatc gttaaccgcg agcgttttga
    13861 ctgggttgcg tttgatgagt cgcgccctct ccccgtctct gtgccttcga tgccattgaa
    13921 gctgaaagtc attctggtag gcgaacgcga atcattgtct gatttccagg agatggagcc
    13981 agagctttca gagcaggcta tttatagcga atttgaagat actctgcaga ttgtcgatgc
    14041 agagtcagta acccagtggt gtcgctgggt gacatttacc gccagacata atcacttacc
    14101 tgcaccggga gcggatgcct ggccggtact tatccgcgaa gcagcacgct acaccggtga
    14161 acaagaaaca cttccgctta gcccgcagtg gatcctccgc cagtgtaaag aggtcgcctc
    14221 cctgtgtgat ggcgacacct tctccggcga gcagctaaac ttaatgctgc agcagcgtga
    14281 atggcgcgaa ggtttcctcg ctgaacgtat gcaggatgag atccttcagg agcaaatcct
    14341 gattgaaacc gaaggcgaac gcatcgggca aattaacgcc ctttcggtca ttgaatttcc
    14401 gggtcatcca cgcgcttttg gcgaaccttc tcgcattagc tgcgttgtgc atattggcga
    14461 tggtgaattc accgacatcg aacgcaaagc ggagcttggc ggcaatatcc atgcgaaagg
    14521 gatgatgatc atgcaagcgt tcctgatgtc ggaactacag cttgagcaac agatcccctt
    14581 ctcagcatcg ctgacatttg agcagtcata cagtgaagtt gatggagata gtgcctcgat
    14641 ggctgaactc tgcgccctga taagcgccct cgccgatgtg ccggtgaatc agagtatcgc
    14701 tatcacaggt tcagtcgatc agttcggtcg cgcccagccg gtcggtggtt taaatgagaa
    14761 aatcgaaggc ttctttgcta tttgccagca acgtgagtta accgggaaac aaggtgtcat
    14821 tatccccaca gctaacgttc gccatttaag tcttcacagt gaactggtga aagcggtaga
    14881 agaaggcaaa ttcaccatct gggcagtaga cgatgtgact gacgcactgc cgttattatt
    14941 aaatctggtg tgggatggcg aaggccaaac gacgctgatg caaaccatcc aggaacgtat
    15001 cgcgcaagca tcgcaacagg aaggacgtca ccgttttcca tggccattac gttggctgaa
    15061 ctggtttatt ccgaactgat cggacttgtt cagcgtacac gtgttagcta tcctgcgtgc
    15121 ttcaataaaa taaggcttac agagaacatg gtagataaac gcgaatccta tacaaaagaa
    15181 gaccttcttg cctctggtcg cggtgaactg tttggcgcta aaggcccgca attgccagca
    15241 ccgaacatgc tgatgatgga ccgtgtggtc aaaatgaccg aaacgggtgg taacttcgac
    15301 aaagggtatg ttgaagcaga actggatatc aatccggatc tgtggttctt cggatgccac
    15361 tttattggcg atccggttat gccgggatgc ctgggcctgg acgcaatgtg gcagctggta
    15421 gggttctacc tcggctggct gggcggcgaa ggtaaaggcc gcgcgctggg cgttggcgaa
    15481 gtgaaattca ctggtcaggt actgccgaca gcgaaaaaag tgacctaccg tattcacttt
    15541 aaacgcattg ttaaccgtcg tctgattatg ggcctggcgg atggcgaagt gctggttgat
    15601 ggtcgtctga tctataccgc cagcgacctg aaagtcggtc tgttccagga tacgtctgcc
    15661 ttctgattat aatcagacag agtatcaaaa ggcgaaacct ccgcaatgcg gaggtttctt
    15721 tttaaagaga cagaatcagg ccattactac cctgtccgcc atggcttctc gccagcctcc
    15781 cagccattgt gacctttgat tcagcgtctg atagggacac atttcttttg agcgtccggc
    15841 gatgccggcc tgataaccac gttgatgtgc ccgttccagg cgatctcgtt tttgtctctt
    15901 catgcctcgt ttccctcata ctggtttctg gtggaaaaga aaacagtgat tactaaatgt
    15961 gcaatcacag ttaagcaata ccgcgaatca taactgccgt caatgcgcaa aattcacgcc
    16021 actgtcatat ttgtgagcta cacgggtatg ccggttgtac aaaaaatgtg aagaggaaca
    16081 actttttccg cacgttagag acaaaaaaac cgcctcaggc aacataccgg aggcggttat
    16141 atttacaaat ctttataatt aaggtagacg ctttatctct tgcgcaatag aagcggcttc
    16201 ctgactccag gcactggcca gtactttaac catctcatcg taaccatcct gagtttgcac
    16261 cccttccaga cggaacggac gtttgatcag ttgtccctgg tggttcaaca gccactcacc
    16321 actgacaatg accttgccat catagcgacc gttaaactcc gtgacggtaa cattgagcgt
    16381 gtcctgggcg cttcccagag gctgggaggc aaccacccag ccgggcagtt gcgtactcag
    16441 gttggcaacc agggtgttgc gcaactgttg atccaacggg ctggcccaca agttgttatt
    16501 ggcaatcaca tacttcacat cactggtttg ataaaccaca ccattccccg ccagatagtc
    16561 aggaacagtg acctgctcta cccataacag acgattgccc tggctggcgg tactttgtgt
    16621 accgctctgc accacaggta actggtaata gtttttatta atttcgccgg agctgcatcc
    16681 ggccagccac agtgctgcaa tcgtcactag ccactttttc attgtttcgc cctcttcggc
    16741 tctggatctt ttttgtcctt cgcttcaaat accagcgcgt tactcttctc attgagcgtt
    16801 ttcagcaccg gttgcagttc tcgcaacacc tgatcaaggc gctgcatatc cgccaccatc
    16861 ttgttgtagg ctgcggagcc aggctggaag ccctgcatgc tgcgattcaa ttcacgcaac
    16921 gttgactgca tatccgtcgg caactgctgc atcgactggc tagcgaggat cttgttcatg
    16981 ctatccagcg tcgtttgcag gtttttcatt gtgcgctgac tttcagaaag cgtactggtt
    17041 gcctgttcaa tcatcggatt caatggcagt ttgttgatct tatccaacgc ttccatcagt
    17101 cgttgctgga tttgcgccag gccgccgcta acggtcggga taatctgata accattaaat
    17161 tcacgaatac cggttattgc aggcgtattt ggatagaaat cgagatcaac atacagtgca
    17221 ccagtgacca ggtttccggt tttcagcgat ccgcgtaaac cacgtttcaa caattcgcca
    17281 aggtgctcaa caacatccgc attttcgcca agctgcattt tcagccgctc tggctcgata
    17341 cgaatcagta ccggaatacg gtaatcatcg ttaaatgtct gacgcatatt cggcgcaaag
    17401 aatggcactt tgcttacggt acccaggcga ataccccgga actctaccgg agctcccggt
    17461 tgcagaccgc gtaccgaatc tttaaagaac atcagataat caatgtgatc ggtgtacaac
    17521 gaatcctgaa tgctcttctg atcatcatac aaaacgaaag ctgttttcgg tgccactggc
    17581 tgccctaaat ccagaccttc cggcacatca aagctgacac cgccactcag cagcgttgtc
    17641 aatgagccca tctccacacg catccctgct gacgtcagat caaccgcaat gccactatct
    17701 ttccagaagc gaacattgtt ggtcaccagt cggtcataag gtgcattgat gaacagttga
    17761 tagctgatat tgcgtttttg tgtatcgaag gtgctggttt caaccgaacc tacccgatag
    17821 ccacggaaca gcaccggatc tcctggcgag agctgcccgg cttttttgct atcgagaatc
    17881 acacggatac ctttcgcatc aggcggggcc aacggtggcg agtccagcaa atcgtattta
    17941 tccattttgc tgcctttcgc gcctggctgc agttcgatat aaaccccaga cagcagggtt
    18001 cccagaccgc taatcccttc tcgaccaatc tgcggtttca ccacccaaaa gacggtgtct
    18061 ttatgcagca atttttccat accggaattc agccgcgctt tgatttcaac gtgcgtcaaa
    18121 tcatcagcca gtgtggcgct ttcaaccacg ccgacgtcaa cgctacggct tttaatggtg
    18181 gttttgccac cttcaattcc ttccgcattc gcggtgatca gggtcacttc cggtccctga
    18241 tggctgtaat gataaaaaag aacccaggcc ccaatgagcg ccgtgacgat aggaaatatc
    18301 cacacgggag accagttctt cactttctgg attttggctt ccccattatt agattccata
    18361 ctgtcaggac tcctcatgct ctgattcagg ttgacgatcc cacgacaaac gcgggtcaaa
    18421 cgtcatagca gaaaacattg tcattatgac gactaaagca aacattaatg cacccattgc
    18481 cggataaata ctcattaaac ctcccatacg caccagcgcc gagagcaccg cgataacgaa
    18541 aacgtcaatc atcgaccagc ggcctacaaa ctcaacaact tcataaatca aatgcattct
    18601 ttcactgtcg cgcttgccat gccctttggc atcccaacac agccacgcga tggcgatcat
    18661 ctttaacgtt ggcaccataa tactggccag aaagatcacc gcagcgacgg gataagagcc
    18721 ttcgctccat aacagaatga ccccagcgag aatcgtcgac ggcatcttcg agcctaataa
    18781 atccgtcacc atgatgggca aaatattagc cggaagatac agcatgatgg acgttacaag
    18841 cagcgcgagt gtccactgca ggctgtttct acgccgaacg taccctttgg tactacaacg
    18901 cgggcacacg ggttcatcag caggaaggat tgccgtacaa caggagcacg aacgcagccc
    18961 ctgacgtatc cccgtgacgc ctggttttag cggctggcgc agttctggca tcggggcgat
    19021 gtcgtcccat aaccagcgac gatcaacgca ctgaaaagcg cgcagttgca ggacacaaaa
    19081 taaacaccag gggagaaagc tgctgcctac cccaatgctg ccgtaagcca tcagtttaac
    19141 gaaactgacc agcacaccag cgaggaaaat ctccgccatt ccccaggttt tgagttgaaa
    19201 aagcacccgt gccagttgct cttttaaacg gaccggtaat tccgcgcgat tcaccagtaa
    19261 cagaatggtt atcagacaaa acgcgggaac cagttgcaca aacaacagga aaaaggtgcc
    19321 gaggctggcg tagtcctcag aaaaaagcac gccgggaatt tccagtaatg taatttcact
    19381 ggtaactcct gcaacgttca tattcacaaa aggaaacaag ttggacagca acagcatgaa
    19441 cagtgcagcc aacgcatagg cggtgggacg ctgccgaggg gcatcccacg ccacggttaa
    19501 cgttgtgcca caccggggac atgccgcttt ctgaccatgc tcaaggcgcg gtaacgccac
    19561 cagcatgtca cactgcgagc acaggatgtg cttcgcggca tgatgatgtt cgcacattgg
    19621 tactccttaa tgatgcgcta ttttacagat gactaacaaa gtgttggttt tcatgcctga
    19681 taaacccagt tcatcaggca attttaccgt tgtcagctgt ctctgtttaa atcgactatt
    19741 ttgcgatcag ccaccatttt ttaacgcttc aagatactcc cagcgttcaa aggcttgctc
    19801 cagctcctgc tctgcagcag ccatatcggc gagcactttt tgtgtctgct catgtggctg
    19861 actgaagaag gaagcatccg ccacctgcgt ctgtagggct tccagcttcg cctccagatc
    19921 ttcgagcaat tgcggtagct gctccagttc gcgctgcaat ttatagctta gtttgctact
    19981 gctgcgtttt acagtttctg cttttgccgc agcggcttct tcggtttttt tcaccgcagg
    20041 ctgtttgagc gccacatact gctcttgctg accacgggca tcatgataac cgccgacata
    20101 acgaccaatt ttaccgccgc cttcgaagat ccaacattct gtaacggtgt tatcgacaaa
    20161 ctgacgatcg tggctaacca gcaataccgt gccctgatag ctgtcgatca gttcttccag
    20221 cagttccagc gtttcgacat caagatcgtt ggtcggttcg tcaagaatca ataagttgct
    20281 tggtttgagg aacaaacgcg ccagcagcaa gcggttccgc tcaccgccag aaagcgcacg
    20341 taccggcgtc atcgcccgtt tcggatggaa cagaaagtcc tgcaaatagc ccaatacgtg
    20401 gcgtggcttg ccgttaacca tcacctcttg cttaccttcg gcaaggttat ccatcaccgt
    20461 tttatcggga tccagttccg cgcggtgctg atcgaaataa gccacttcca gtttggtgcc
    20521 aacgtgaata cgcccgctgt ccgcttgaag ctgaccgagc atcagtttca gcagcgtggt
    20581 tttgccgcac ccattcggac caatcagggc aattttgtcg ccacgtagaa cctgggccga
    20641 aaaatctttc accagttgct taccgtcaac ctggtagcaa acgtcttcca tttcgaaaac
    20701 gatcttaccg gagcggctgg cctcttccac ctgcatcttt gcggtaccca tcacttcgcg
    20761 acgttcacca cgctcgcgac gcattgcttt cagggcgcgt acgcggcctt cattacgggt
    20821 acggcgtgct ttgatcccct ggcgaatcca cacttcttcc tgcgccagtt tgcgatcgaa
    20881 ctcggcattt tgtagttctt ccacgcgcag ggcttcttct ttttcctgca ggtactggtc
    20941 gtaattcccc ggataggtca ccagcttgcc gcgatcgaga tcaacaatgc gcgtcgccat
    21001 attgcggata aacgaacggt cgtgggagat gaaaataatc gtcccattga aggttttaag
    21061 gaacccttcc agccagtcga tggtttcgat atccagatgg ttagtcggtt cgtccagcag
    21121 caatacgcgc ggattgctca ccagcgcgcg tcctaatgcc gctttacgca accagccgcc
    21181 ggaaagcgac gacagcgcaa cattaggatc taaccccagt tgcgccagca cttcgttgat
    21241 gcggttttcc agctgccaca agttgtggtg atccagctgt tcctgaacct tcgccagttc
    21301 gttgagattt ttctcgctcg ggtcgttcat caccaggcgc gaaatatcgt gatagcgttt
    21361 cagatattcc gcttgttctt caatgccttc ggcaacgaaa tcataaacgc taccctcaac
    21421 gttacgcggc ggatcctgtt gcagacgagc tacaatcaaa tcttgctcgt aaataatgcg
    21481 accgtcatcc agcccttgtt cacggttgag gattttcatt aacgtcgatt tgcctgcgcc
    21541 gttgcggcct accagacaaa cacgttcatt atcttcgata tgcagttctg cgttatcgag
    21601 aagcggcgcg tcgctgaacg acagccaggc gccatgcata ctgattaatg acattactat
    21661 tcctttcagg ctgcggtaat cagccagcag ttgtggatct ggcggttacg ggcgaaatcc
    21721 tgggagagcg ttttttgcgt aatttcttgt gctttcagtc ccagttttgc caggccgccg
    21781 agatccatac ggaagccacg tttgttgttc gagaacatga tcgtcccacc tgcacgcagc
    21841 agacgtttca aatctttcat cagcgccaga tgatcgcgct gaacatcaaa cgcatcttcc
    21901 attcgttttg agttagagaa ggttggcgga tcgataaaga tcagatcgaa ctgttcattt
    21961 gcctcacgca gccacgccag gcaatcggcc tgaatcaggc gatgcgcacg cccggtcagg
    22021 ccattcagac gcaggttgcg ttctgcccac tccagataag tacgcgacat atccacggtg
    22081 gtggtgctgc gtgcaccgcc taatcccgca tgcacggtgg cgctgccggt ataagagaac
    22141 aggttgagga aatctttgcc tttgctcatc tggccgagca tacgacgggc gatacggtga
    22201 tcgagaaaca gaccggtatc gaggtaatcc gtcaggttca ccaacaagtg agcgttatat
    22261 tcggtaactt caagaaactc gcccttctcg cccagtttct ggtactgatt tttgcccttc
    22321 tggcgttcac gggttttcag caccagtttg tttggcgcaa tccccagtac cgaaatggtt
    22381 gcagcgataa tatcgaacag acgctgacgc gctttgtgag catcaatagt ttttggcggc
    22441 gcatactcct gcaccaccac ccagtcggca taacggtcaa cggcaacgtt atattccggt
    22501 agatcggcgt catacaggcg gtaacattca attccttcct ggcgagccca cttctcgaat
    22561 tttttgaggt tcttacgcag gcggttggcg tagtcttccg ctaccatcgc cggtttgctg
    22621 tctggagtgc tttcggcaac gtggtaattt ttctgtacgc agtccagcgg gccgtttttc
    22681 gccttgtact gtttgtctgc acgcagctgc aagcagctta gcagatccgg cgaggcacta
    22741 aacaaagaga gattccagcc accaaactgg tttttcatga tccgacccag caggctatgc
    22801 agcgcaatca gcgccggttc gctgtccaga cgttccccgt atggcgggtt gctcaacact
    22861 gtgccgtacg gccctttcgg cagcggattg gtcagttgcg cgacatcttt cacctcaaag
    22921 gtgatcagtt caccaatccc cgcaagacgg gcgttagtgc gtgcacgttg aatcacccgt
    22981 gcgtcgctgt ccgaaccgta aaaatgagag ctatactcag ccaggccttt acgggcgcga
    23041 gtttgcgctt ccgctttcac ttcctgccag atagcttcat catgctgcgc ccagccgcta
    23101 aagccccaac gcccacggtg caagcctggt gcgcgatcgg tcgccagcat cgccgcttca
    23161 atcagcaacg taccggaacc acacatcgga tcgagcagcg gtgttcctgg ctgccagccg
    23221 gatcgcatca caatagcggc tgccagggtt tctttgatcg gcgcaatacc agcacgatcg
    23281 cgatagccac gcagatgtaa accatcacca ctcagatcaa gagcgatact ggcggtttct
    23341 ttatgcagcc agacgttaac gcggatatcc ggcgcatcgc gatcaacatt tggacgcggc
    23401 agatttttcc gcgtgaaagc atcgacgatc gcgtctttca ctttcatcgc gccgtactga
    23461 ctgttgcgga tggtgtcatt caaaccactg aagtggacag cgaaggtcgc gccaggatta
    23521 aacatctctg tccagttgat cgcctgaaca ccgagataga ggtctaaatc gctgtaaacc
    23581 ttacactcgc ccagcggcaa cataatacgc gaggccaggc ggctccacat caggctctgg
    23641 taaacaagcc gtgtgtcgcc cttgaaatgg accccaccct gaaccacctg gcattcaacg
    23701 gcccccaggt tttccagttc agtttttaac agctcttcca gcccacgggc cgtactggca
    23761 aacagagaat tcatatcgtc acttttactc taagagccta ccgcagtagg cgtaattgtt
    23821 gcagtcagtc tggacatgga cagcgcggag aaaccggagc gtacacaagt acgtgaggat
    23881 ttcgagcact tccctgggcc aaactgacaa ataaaatagc ctaacgggac aggcgctaag
    23941 aaaattgttg cgcattatag ctaataagcg cgtcatgtca taaagttgag ggcttatttt
    24001 catttgagga ccgcaccgtg gcgacattaa cccggctttt tattcatcct gttaaatcga
    24061 tgcgcggcat tggtcttaca cataccctgg cagatgtgag tggtctggcc ttcgatcgca
    24121 tctttatgat cacggaacct gatggtacgt ttattactgc tcgccagttt ccgcaaatgg
    24181 tacggtttac tccttcacct gtgcatgatg gcttgcatct caccgcacca gatggcagta
    24241 gcgcatatgt tcgttttgct gatttcgcca cacaagacgc accaaccgaa gtttggggca
    24301 cacattttac cgcgcgaatt gcgccaggcg cgatcaacaa atggctaagt ggatttttct
    24361 cccgcgaagt gcaattacgc tgggtggggc cacaaatgac ccggcgcgtg aaacgccaca
    24421 acactgtacc tctgtcattt gctgatggct atccttacct tcttgctaac gaagcctcgt
    24481 tacgtgatct ccaacaacgt tgtccggcca gtgtaaaaat ggagcaattc cgccccaatc
    24541 tggtggtttc cggcgcgtca gcctgggaag aagatcgctg gaaagtgatt cgcattggtg
    24601 atgtggtgtt tgatgtggtt aaaccttgta gccgctgtat tttcaccacc gtcagcccag
    24661 aaaaagggca aaaacatccg gcaggcgaac cattaaaaac attgcaatct ttccgcactg
    24721 cccaggataa cggcgatgtc gattttggtc agaatttaat tgcccgtaat agcggcgtga
    24781 ttcgcgttgg cgatgaggtg gaaattctgg caacggctcc ggcaaaaatt tacggcgcag
    24841 ctgccgctga tgataccgcc aacatcacgc aacaaccgga cgcaaatgta gatattgact
    24901 ggcagggaca ggcatttcgt ggaaataacc aacaggtgtt gctggagcaa ttagaaaatc
    24961 agggaattcg tatcccttat tcttgccgcg cgggcatttg tggaagttgc cgtgttcagc
    25021 ttttagaagg cgaagtcacg ccgctaaaaa aatcagcaat gggcgatgat ggcaccattc
    25081 tttgctgtag ctgtgtaccg aagactgcac ttaagttggc gcgttagact gcctgttcga
    25141 ggctgaagct gtcaacatta acctgcggtt tcagcctgtc gttcatgatt ttaatggcgt
    25201 cgcccaactg catcgcgcga cccgcaataa caacgcaggg ttgtgccagc aggcatagtg
    25261 cggcattttc gccagattca acgaccagca aattgacctg ctcgtgagta tcgctcaacc
    25321 aaacgcaggc cgcatcgccc ggcatgggca cccacatttc accatgggaa acaaaatgcc
    25381 agcttttcgg catttgcggt ttgagatagc gaattgctac cagtgcattc agcaccagtt
    25441 cggctttttg ttctttagat aaattaaaat cgcggcactt ttcttcaaaa gagaaataga
    25501 gcgcggcgtc gtcaacgcaa aagcctgcgg gagaaaaggc atctggagtc aacatcttgc
    25561 gcgcaaaacg tgagcgaaat agcataccat tggctaaatc gagcatcatt cgatcgtgct
    25621 cttcatcgta ataccaacgc cagttatcgt ctggtttaat tcgcatctgc ctctccccta
    25681 ccctataaaa ccgcagcaac acaatccatt gtcgcttcgc ttaataacct caacaataaa
    25741 agtacaaaat gtctaaataa gcaacagcga tgaatataaa acaaccaggg ctgaaaataa
    25801 agccctgatt atcttataaa gaggaattcg aagaaataat tagatatggg taacgatttc
    25861 tttaatcagc ggcggacctt taaaaataaa accagaataa atttgcacca gtgaggcacc
    25921 cgcagcaatc ttttcacgcg cagcgataac cgagtcgatg ccgccaacac cgatgatcgg
    25981 taagcgaccg tttaattcca gtgacaagcg gcgaataatt tcggtgcttt ttaactgaag
    26041 cggacgacca cttaagccac cggtttgatc gcaatttttc attccctgaa caagagaacg
    26101 atcgagtgtg gtattggttg caataacgcc atcaatatta tggcgaacta aactatcggc
    26161 aacctggatc aattcttctt cagaaagatc cggcgcgatc ttcactgcga tcggcacata
    26221 tttatggtgc atcgcttgca aatcattttg cttattttta atcgcggtta agagatcatc
    26281 cagcgcttca ccatattgca gcgtgcgtaa tcctggggta ttcggcgatg aaatattgat
    26341 ggcgatatat cccgcatagg catagatttt ttccatacaa atcagatagt catctttgcc
    26401 ctgctccact ggcgtatctt tatttttgcc gatgttaata cccaggacgc cgtcataatg
    26461 ggcctttttt acgttctcta cgaggttatc aacgccaaga ttattaaagc ccatacggtt
    26521 gatcaaacct tcggcatcta ccagacgaaa gagacgcggc ttgtcattac ctggctgtgg
    26581 acgtggcgtg acggtaccga tctcgatcga tccaaatccc atcgcgccta acgcgtcaat
    26641 gcactccccg tctttatcaa gaccggctgc cagaccaagc ggatttttaa acgtcaggcc
    26701 catgcagtta acaggtttcg caggcacttt ctgccgcacc agtgcttcaa acggcgttcc
    26761 tgtaatacgg cgtaattgct gaaaagtaaa ctcatgagcg cgctctggat cgagctggaa
    26821 aagggcttta cgaacgaagg ggtagtacat gaactctcct ggattcccgg tgtgcaaacc
    26881 gggggcgtat tatgtgcgag ttcggaccaa aagggaattg acctgcggca aaaaaaacgc
    26941 aaacgttttc cgaacctgtt tcagggcaga ttttgctcct tctttgcact caacccgccg
    27001 taatcattta tcgcccgcca ggcgacatga cacggtgcga ttgcctgaca ccagcttgtc
    27061 tgacgctggc ttttaggcgg cacaactccc gcattactta acgctttacc atttacgctc
    27121 aactgaatca ggttaatgta gtaaggcgtt gggttagtga gttgtacacc ctgcgaattc
    27181 cgtgtccagc gtaattgttg ataagcttcc agcgggtcgc ccggcaaacc ttcgggtcgc
    27241 cagaacagtt taaataccga gcgcatcgcc acttttacgc tctgattttc aactttgccg
    27301 gatggcacgc tggcaatgct taactcaaat agcgtttcgc gatccacagg caagatgtcg
    27361 ctttccgttc tcagcaagcg caacgtgcct gtagtaccgg gcttcaggag aataagtggc
    27421 ggagcggcca gtaatggtgc tggcactgtc gacgcttccc ccccggccca acgcgttggg
    27481 cggttgattt tactgttaat aagccaggat tcctgactgg tattagtcag taaaatagat
    27541 atcgattctc tgtctgccgg aaagataaat cgcgttccgc cgaccaccac tcctgcccgc
    27601 aattcacccg ccaccagcag cgataaaacg gcgaaaatca agagcgccaa tctgttccac
    27661 gtattagtca taatgaagta caaatgtcgc gctggcattc actttacccg actgcacatc
    27721 gctattgaca ggtcgcaatt gcgcataaaa agttaatggc accttaccgc tatgtaggct
    27781 gtagtcttta ctcgcctggt taagcggaat caatatcttc gcatcatcca gaatcgcaat
    27841 tgccagcccg ctggctgcat ccataccact ttcaatggac agcatttcgc tctgcactcc
    27901 gtcgggggtt cctgaaaagg tgacgtcaac cgtgctgaca tctgttccgc aatcctgcaa
    27961 attaatgatg aaagatttct ccgggccata attcccgtga gaaaaacgtg tcagcgagac
    28021 atcgcctaac tgcacattga tttgcttgct atcctgtgcc agcacgcatg tattacgacg
    28081 aaacgatccg ctaatgttga ccgtgccatc atgagcaaat aactcactac tccccgttaa
    28141 tcccagtaag cacagaatcc agattatccg tttcaacatc actccttcac tggtaaacca
    28201 gatcaaaata gagaaccgcc gtcgcattac cgcccgttgc tcccgccttt gtggacttat
    28261 aacgaagttg atatttgagt gtgttatccc cggcttttaa gggcgtaaga ggctggacct
    28321 gattgagcgg gatttcttgc tgactttgcg catcaagaat ttgcacggca atccccgttg
    28381 ccataccacc gcttccgcct gtgtcagtta gtttaagcag tgtcggcgca agggtgtcag
    28441 cctcgccgct aaaaaggacg tccgcgccag taataccagc ggcacaaccg gtgatatcga
    28501 tattcaaatc agctgcagtg cttacggtat tcgcggcctg aaaatcactg atattaaaat
    28561 cgccaatatg aatattttgt aatgcacttt tggtggcgac atcacagccc tgatcgacca
    28621 cattcccata aatatgcata ctgacttgtt caggcccagc catcccgaca tggatgttaa
    28681 gaaggctaaa gaacaaaata acgtactgat atatcgtttt tttcttcata cgttcacgcg
    28741 ttaatcaaaa tttattctca ccgttgccgt ggcttcaaat cgcccggttt ctggtggtac
    28801 gcctgtcgtg ctgactggat agacattcat tcgcgcgata ctgtctgcat catcaggaat
    28861 agagagcggt agtgtcccac cagcagggga aataatgttg ttctggctat cggtcaccac
    28921 cacgcccacg ccaggacgtg acgtcttaat cgcctgggga tagctggggt ctgaggttgc
    28981 cgtcagactc aggttgacgt taactaaccc ttcgtttccc gtgttacacc tgactggaat
    29041 tgcgagttct gccgttctgg ccccaagggg tttatgccct acaacacgaa agttagccgc
    29101 aaatacgtcg cccagatcga cctcgatagt ctctccggca ttgacggaac atgagccaag
    29161 tgaattgatc gtaccagaca ggtaatacac taaaaccggt gtacctgtag tcgtgcagga
    29221 atcacctgcg ggtatgttgt agcattcaaa taatcgcgcc agcgccatag gaggaataac
    29281 gaccgaacca acgaacggct gcaatatttt cagggaaaag ctaccgctat taccgctagc
    29341 cccctggcca cctaaacgtt gaccgccggg atcgcaaata tgccatcgtg tatctgcccc
    29401 tgtgcccacg ttttcgaaag gaaccgcagt aggagaactg cctttaacct gcaataaaac
    29461 tttggcggca agatagtcat taattttgta ccagttgtcg cctaaataga ctaacggtga
    29521 atccgcggcg aatgcccatc ggccacttct gacattgtct ttatcgcaat cacaggaaac
    29581 ataataatca ttcgattcgt tccactgata aaatttgtct ttaactactc cagccgtatt
    29641 ttcttcttcc tccagggttt taacgccaaa attgacgcca atatacggat tcccctgagt
    29701 ggtatgacac tgcccgctat aagcagcagt ttgagcgcac catagcatta agacggcggc
    29761 aaaaaatagt cgtagtaata acacgcattt ttctccaaag attatctgca tatggcatcc
    29821 attcgcgtaa tcgcagagtt aatttgttgt tcggtaagct ggtaactgat atgacaaatt
    29881 tgatcgggag cttcgcccca gcgtacagtt atcgctccct gagcgggcaa accggacata
    29941 tagagcgagc catcatcatc aataatgccg ctattagtct gtatgccgtc cagcgtcgct
    30001 atcgcgccaa aacgcagcgg tataccctgc tttgatgttt tcattaatac ccttgctccc
    30061 gcatgggcat tgaactccgc cagaaccaac gcccctttgg tgggaaccac gttgactact
    30121 gcattttcaa gatccacgtt acgttttaac gatgccgcat caagtgcgat tcgattttcc
    30181 cgataagaag tggcataggg gacaacggta tatccgcgcc agtcagtttt cagcccggtg
    30241 ctgttcgcca cacgagtatt ctctgcgcct ggtgctgcaa tcagcacgtt agtttccccc
    30301 agcgattgcc ccagggtaat gccctgtgaa tgggcaacta aactgcctga aagagcgtag
    30361 ttcagttgtt gttgactgcc gttatcgctg tagttgtagc ccactcgggc atctgcaaac
    30421 gcccctttgt agtccatgct ggcgctacca ttagccgttt tcccctcgct gttatatccc
    30481 tgttgcacgc tgtagctcag gttaccgtca tcgagtagtg tctccgttaa acccaggttt
    30541 tgtgtaatgt gcccttcgtt atcgatactg gtgttagagg tcatccaggc gtaatttttt
    30601 ttcctggtat agctatcatt acttctgggg agcaacagat tgagcggcag ggaaaaattt
    30661 agtgcaaaca cctgatcagc atcaggttga ccacgggact tactgtagtt ccaggaaacc
    30721 cccagcgaga gatctttaat ttgcgtgttg tagccaaact gtaatagccg atcctgttga
    30781 tcggtatgcc agtaagtttg ctggctacca cttaaataaa acgaaccgta ctcgcctaat
    30841 tgctgggaga tattgacctg cagtttgcca cgtttggtgt aaaacaaatt gtaataacgc
    30901 gaccacatcg gcgtatcttc atcatcaccg tcattaaatt cgtagccatc catatgttta
    30961 tacatggtgt cggaaagggt gtagaaaccc gacgtcgaat agcgatagcc catcaattga
    31021 aaattagtgc ccaattggtt gagcgttttg gcatacagaa aacgatatga ctgaccttta
    31081 tgttcgcttt ggtctgccag cgtactcttc gcctgggtcg catcgaacga aattgctccg
    31141 aaatcgccaa ggttaaatcc cagaccaaac atggcggcac ggtaatattc agcatattgt
    31201 ccaccaccat accatgtcgt tccccacggt ccgccccact gcaacgtggc ctgggcaaat
    31261 ttgctctcct gctgttcatt actattggtt ctgtatttcg ccagtgtcac cgcgtatttg
    31321 attcgcccct gacgctggag taatggcacg ctggaaaacg gtacgctgta gctattgacg
    31381 ctgccgtccg cttctttgat ttcaactaac aaatcaccgc tcgacgacgt ggaatagaga
    31441 tcactaattt caaaagcacc aggcgataca taagtttggt aaatggtgta accattttgc
    31501 ttaatcgtta tctgggcatt gcttttggcg ataccacgca ccacaggcgc aaaccctttc
    31561 aggctgtctg gcagcatgtt gtcatctgac gccagctgca caccacgaaa gctgacacta
    31621 tcgaaaaaat cgcctgcagt ataatcatca cctacggtca gttcgccttt tagtggaata
    31681 accgcccgct gcaaatagct gctgagattc ttccattctg cggtttggcc actactgcga
    31741 ctccatgttg aattgttgcg caatctccat gggcctaaat taacgccact gtttaaattc
    31801 agaaaatagc tgtcgccaga atcactgtct gcgctgctat gaatactgtt agccccgcta
    31861 aacgaatatc ccagtaatag cgcgttgatc ccttcatccc aacgttcagg ggggacataa
    31921 ccacgggcat tacttttgat ggcgatttgc ggtactgaaa tttccagacg gagtttattc
    31981 acattaaacg ttacgctggc atccggaata atctccgcca atggcacaca cgcttgtttg
    32041 tccagttgtt taaaagccgg aaacgcatca gtattaacac ccattgcatc caggctttca
    32101 gtcgtcaaac aggcggcaat ccccttctct ggcgttatct caacaaaatt gacgttgcgc
    32161 gtatctacaa ttgtctgatt cagaacaata tcgacacgat aaacccctgg cgttattttt
    32221 tgtccttttt cgaaacgaga caaatcggcc accattgagg gatcatcttt gaaaaaagca
    32281 ggatcgaacc aggtttcagc ttgtgcattg aacgctgccg acacaaacac caccagccca
    32341 ccaataaacg atggcactcc accagagctt aacaggctgt gttgtcgatg agttctatac
    32401 atctcgctct cttaactgaa gacggcttac gatttacagt tgagttgcgg cgtttccgcc
    32461 ccgtagtcat tgatggttcg atacgttacg ctgctatttt tggccccctt tggcagagaa
    32521 aattggtatt tatcaaaggg ggaaatcatt tgatttttaa ctacttttcc acctattttc
    32581 aggtctgtca gggtaatgaa ataaggtgtt gggttactga ccgtaaccat gtcagctgac
    32641 gaagtgcaac gcagtttcgc cggagcctcg cctaagggat acgctaaacc ttttggtcgc
    32701 cagaaaagtt tgatgacgct ttgtgtggca atcttcaggg tatttccctc ttcccgcttc
    32761 gttgttgaag ggactgcccg tacgctagtg aagaaaaggg tttctctgtc tttcgccaac
    32821 ggcgcaccaa tgtacataat gcgtaacaga ttctcattag ccggattcag cacaaatagc
    32881 ggtggtgtaa taataaaatc tgctgatttt ttattatctg gggtcatcac ccatgactga
    32941 ataagataag gaacatccgg actggtgtta cgtacaccca gtgaaatttg ctcttcacct
    33001 tctgaataaa caagacgtgt tgctgcaagt ccaatcccgc ctttacctgc cgcccatgcg
    33061 gatgagcagc tgagtaatat tgccgttaaa cttacggtca caattccctt tgttatgcaa
    33121 gttttcataa atagtcactc ttaaaaaata tgaatatccc ctccagaaca ctagaggggt
    33181 actaattaat aaaacaatta atgagttcta ttaataatag ttaatatgga aatttgttac
    33241 cgcattcaca ttaccagaag tcgctttccc cgtagcgata taatcaactt taaaggtcac
    33301 tgtttgctca gcagaatccg ctaactgaat attcgacgtt tcggtaccgg gagttaccga
    33361 tgtagaacca tctggcagat acatttgtag agcaacattc gtcgctgcat cggtggtagc
    33421 ctggttggca aatgctgtag gttgcacgcc gtcagccgta ccactaaagg taaacgtcgc
    33481 atttttagtt acagtaacat cacagccttc taaggcaatt ggtacatcag ttttaacgcc
    33541 agagactgct ccattagttt gcaacttggt ggcgctgaca tttggcaatg tgactactga
    33601 atctttcttc gcagctacca gggtacaagc tggagcaata actttaccat taaatgttac
    33661 tttgccatcc gtgatagcat tatcatcagc tgccataacg ctggacgttg cacataccac
    33721 agttataaac gccgtcaata cacttttttt catcgtcatc atcctgatct cttcgtaaat
    33781 atgataatgc ctttaaatga cattagactt aataataaca ataattgacc gccatttaaa
    33841 tcaagcatac taaatccaat aagtgacaaa tctttaattt atagatttca tgaatattta
    33901 aatattaact gaacgagtaa ttcattgatt caacatctaa tgactatcca acaaataaat
    33961 atgcgtgata tataaagccc tttctttagt ttattttcag aaaaagatac acaaaa
//
LOCUS       DAZKGS010000032        33983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKGS010000032 DAZKGS010000000
VERSION     DAZKGS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..164
                     /gene="xisR"
                     /locus_tag="ACHOQY_001565"
     CDS             <1..164
                     /gene="xisR"
                     /locus_tag="ACHOQY_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9677694.1"
                     /translation="KKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQA
                     SARPRRHLT"
     gene            199..1479
                     /locus_tag="ACHOQY_001566"
     CDS             199..1479
                     /locus_tag="ACHOQY_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9677695.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHOQY_001567"
     CDS             complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHOQY_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP9677696.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHOQY_001568"
     CDS             complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHOQY_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP9677697.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHOQY_001569"
     CDS             complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHOQY_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9677698.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHOQY_001570"
     CDS             4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHOQY_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9677699.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4955..5515
                     /gene="speG"
                     /locus_tag="ACHOQY_001571"
     CDS             4955..5515
                     /gene="speG"
                     /locus_tag="ACHOQY_001571"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9677700.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHOQY_001572"
     CDS             complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHOQY_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9677701.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHOQY_001573"
     CDS             6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHOQY_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9677702.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHOQY_001574"
                     /pseudo
     CDS             6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHOQY_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHOQY_001575"
     CDS             9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHOQY_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9677703.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHOQY_001576"
     CDS             11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHOQY_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9677704.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHOQY_001577"
     CDS             12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHOQY_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9677705.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHOQY_001578"
     CDS             13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHOQY_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9677706.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14049..15341
                     /gene="clcB"
                     /locus_tag="ACHOQY_001579"
     CDS             14049..15341
                     /gene="clcB"
                     /locus_tag="ACHOQY_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9677707.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHOQY_001580"
     CDS             complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHOQY_001580"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9677708.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHOQY_001581"
                     /gene_synonym="dgsA"
     CDS             complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHOQY_001581"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9677709.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17469..18362)
                     /gene="ynfL"
                     /locus_tag="ACHOQY_001582"
     CDS             complement(17469..18362)
                     /gene="ynfL"
                     /locus_tag="ACHOQY_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9677710.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18469..19722
                     /gene="ynfM"
                     /locus_tag="ACHOQY_001583"
     CDS             18469..19722
                     /gene="ynfM"
                     /locus_tag="ACHOQY_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677711.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(19771..19890)
                     /locus_tag="ACHOQY_001584"
     CDS             complement(19771..19890)
                     /locus_tag="ACHOQY_001584"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9677712.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20146..20454
                     /gene="asr"
                     /locus_tag="ACHOQY_001585"
     CDS             20146..20454
                     /gene="asr"
                     /locus_tag="ACHOQY_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9677713.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            20547..20630
                     /locus_tag="ACHOQY_001586"
     CDS             20547..20630
                     /locus_tag="ACHOQY_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677714.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            20730..21551
                     /gene="ydgD"
                     /locus_tag="ACHOQY_001587"
     CDS             20730..21551
                     /gene="ydgD"
                     /locus_tag="ACHOQY_001587"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9677715.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21590..21919)
                     /gene="mdtI"
                     /locus_tag="ACHOQY_001588"
     CDS             complement(21590..21919)
                     /gene="mdtI"
                     /locus_tag="ACHOQY_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9677716.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(21906..22271)
                     /gene="mdtJ"
                     /locus_tag="ACHOQY_001589"
     CDS             complement(21906..22271)
                     /gene="mdtJ"
                     /locus_tag="ACHOQY_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9677717.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22683..22826
                     /locus_tag="ACHOQY_001590"
                     /pseudo
     CDS             22683..22826
                     /locus_tag="ACHOQY_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(23546..23827)
                     /locus_tag="ACHOQY_001591"
     CDS             complement(23546..23827)
                     /locus_tag="ACHOQY_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677718.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(23841..25502)
                     /locus_tag="ACHOQY_001592"
     CDS             complement(23841..25502)
                     /locus_tag="ACHOQY_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9677719.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(25486..25842)
                     /locus_tag="ACHOQY_001593"
     CDS             complement(25486..25842)
                     /locus_tag="ACHOQY_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP9677720.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(25965..26147)
                     /locus_tag="ACHOQY_001594"
     CDS             complement(25965..26147)
                     /locus_tag="ACHOQY_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677721.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(26131..26571)
                     /locus_tag="ACHOQY_001595"
     CDS             complement(26131..26571)
                     /locus_tag="ACHOQY_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9677722.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREDEERAANRNNWMYDADE"
     gene            complement(26571..26864)
                     /locus_tag="ACHOQY_001596"
     CDS             complement(26571..26864)
                     /locus_tag="ACHOQY_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9677723.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(26861..27199)
                     /locus_tag="ACHOQY_001597"
     CDS             complement(26861..27199)
                     /locus_tag="ACHOQY_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9677724.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWLDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(27196..28407)
                     /locus_tag="ACHOQY_001598"
     CDS             complement(27196..28407)
                     /locus_tag="ACHOQY_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9677725.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNMEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(28409..28981)
                     /locus_tag="ACHOQY_001599"
     CDS             complement(28409..28981)
                     /locus_tag="ACHOQY_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP9677726.1"
                     /translation="MGEKIMKSMEIRSSEITTSGSNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(29021..30178)
                     /locus_tag="ACHOQY_001600"
     CDS             complement(29021..30178)
                     /locus_tag="ACHOQY_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9677727.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(30470..30694)
                     /locus_tag="ACHOQY_001601"
     CDS             complement(30470..30694)
                     /locus_tag="ACHOQY_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677728.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            complement(30735..30863)
                     /locus_tag="ACHOQY_001602"
     CDS             complement(30735..30863)
                     /locus_tag="ACHOQY_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9677729.1"
                     /translation="MVLVMKYGAITRTANHTAGEIRLFLHQLKRYGALPGFRHGLA"
     gene            complement(30820..31092)
                     /locus_tag="ACHOQY_001603"
     CDS             complement(30820..31092)
                     /locus_tag="ACHOQY_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9677730.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQVTMRRL
                     ECCRKAQRPPEPPDNYGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            complement(31103..31513)
                     /locus_tag="ACHOQY_001604"
     CDS             complement(31103..31513)
                     /locus_tag="ACHOQY_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9677731.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAALPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            complement(31510..31788)
                     /locus_tag="ACHOQY_001605"
     CDS             complement(31510..31788)
                     /locus_tag="ACHOQY_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677732.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(32077..33831)
                     /locus_tag="ACHOQY_001606"
     CDS             complement(32077..33831)
                     /locus_tag="ACHOQY_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFP9677733.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLARERQNLSDGLVIELPVKQKAQLSQMADSERAQLLADRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            complement(33828..>33983)
                     /locus_tag="ACHOQY_001607"
     CDS             complement(33828..>33983)
                     /locus_tag="ACHOQY_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9677734.1"
                     /translation="YEYDCVMPVYQFRRDFSLVQAAPRSKPTSRKKARANIQEIKKML
                     NVFRGKK"
BASE COUNT         8256 a         8894 c         8146 g         8687 t
ORIGIN      
        1 taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg
       61 acggtatgcc atgggataac agtccatgct tttataacct ggaagaaatt gatcgctgga
      121 ttgagcgcca ggcatcagcg agaccaagac gtcatcttac ttgactaaaa gccacactaa
      181 ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta
      241 cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa atctgggggt tcctgacaca
      301 tcaaaaaaca ggcgcgttgc aggtgaacta cgctcctctg tttgttacgc aataaaaact
      361 ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa atttggtgag
      421 gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttttggcact gaaagaaact
      481 gaagtcgcca aaacatcact caacacatac cgtgccgtca tcaaaaatat cctgagcata
      541 atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga ggttcgtaaa
      601 gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca accagggaga
      661 tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg ccgtgttcca gtttggtgtt
      721 gataacggtt acctggcaga taatccgttt aaggggatct cgccattaaa ggaatcaaga
      781 accattccgg atcctctttc gcgggaggaa tttatccgtc ttatcgatgc gtgcagaaat
      841 cagcaagcaa aaaatttatg gtgtgtttct gtttatactg gcgttcgccc tggtgagctg
      901 tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat caggagaaat
      961 ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg ggtcattcat
     1021 cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag actgagcaaa
     1081 gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca aaaatgcacc
     1141 tttgtttttc aacctgaagt gtcagcgaga gtaaaaaatt atggtgacca ttttaccgtt
     1201 gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg ccatcgaaaa
     1261 tcatatcagt cgagacatac ttatgcctgc tggtcgctga cagctggtgc taacccggca
     1321 tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt atacggaaaa
     1381 tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt aagcaagttt
     1441 gccccaacca tgccccataa cgaagcaatg aaaaattaat ttaatattta tcaaatagtt
     1501 aacacgcatg actcttgaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     1561 ctgctttcac caaatcctta gtgcttcttt catgtttttc tattgtcata atggttatct
     1621 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     1681 tgagcaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg gcatcagcaa
     1741 catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac cctttactta
     1801 accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa atagagagtt
     1861 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     1921 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     1981 ccgcatcgat aattaatgtc ggcttgatgc ctttttcagc gaaaatctcg ccaagcggtg
     2041 tctggctgtt attaatcgcc cagtctgccc cgctctcttt cgctttttcc agtcgttcat
     2101 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     2161 tcgtcaggcc gattggaccg gcaccataaa ccagaacggt atcattttca gtcggttgac
     2221 catgaccggt aacgttagcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     2281 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     2341 caccgtcagc gtgcacacct aatacagcaa gtgtcgtaca aacgttcggc ttacctatag
     2401 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgc tcaccgactc
     2461 tggcgctttc cacaccttca cccaccgcat caatgacgcc aaagaattca tgaccaatga
     2521 cgcgcggata tttcgcaaaa ggattatgcc cacggtaaat atggctatct gaaccacaaa
     2581 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcacgtt
     2641 cgataatcga cagttgattc ggtttttcaa ttaaaatgct tttcattacc ttactcctta
     2701 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
     2761 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
     2821 catatagccg ttatcgaaag tccagttgtg cgggaagact tcgagcattt gttcggaata
     2881 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
     2941 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacagcga
     3001 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
     3061 gatataatcg atgagttgct cttcaatcag ttgtttgcag tcccagatgc tgttgaagac
     3121 ttcacccact gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
     3181 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
     3241 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
     3301 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
     3361 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
     3421 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
     3481 tttgaatccc agctcctgat gacgggcata atcatccaga gcttcatcaa tactgtgacc
     3541 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
     3601 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
     3661 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
     3721 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
     3781 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
     3841 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
     3901 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
     3961 gagctactac catacaagca taaagatcga aaaaagccgg agtgatcaca aaaaaaggcg
     4021 tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga ataccttttg tcagctgaca
     4081 cttcctctta tcttattgat aaaatggatt tatgttccta cgtgcgcccc cagcccgcaa
     4141 caatgatcaa catgccgcaa agcgcaatca acgcacccgt ccagtcataa agagtcagtt
     4201 tcacgccatc cacaacgcgc agccacatca acgccgtgca gacataaacg ccaccataag
     4261 ccgcgtaaac acgcccactc gccgctggat gcaacgttaa caaccagaca aacagcgcca
     4321 gtgaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
     4381 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
     4441 tcatctttgt ctcttattga catcatgtat agttataggg cgacataata tcatcaatat
     4501 aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt gtagaatcac ctttaatcat
     4561 tcatacaggg aatgaattat gaaaatcact ctcagcaaac gaatcggcct gctcgctatt
     4621 ctgctgcctt gcgcactggc attgagcaca actgttcatg ccgaaactaa caaactggtg
     4681 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
     4741 aatgacacgc gcaatctgcg ccagaaagtg aataaacgca ctgaaaaaga gtgggataaa
     4801 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
     4861 tgggagccca atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
     4921 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
     4981 acgcccgctg gagcgtgaag atttacgcta tgaacatcaa ctcgacaaca acgccagtgt
     5041 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     5101 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
     5161 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
     5221 tatctccccg gagtatcagg ggaaaggtct ggcaacccgt gccgccaaat tagcaatgga
     5281 ctatggcttt accgttctca atctctataa gctgtatctg atcgttgata aagagaatga
     5341 aaaagcgatt cacatttacc gcaagcttgg cttttcggtt gaaggtgaat tgatgcacga
     5401 gttctttatt aatggtcaat atcgtaatgc cattcgcatg tgtatattcc agcatcagta
     5461 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
     5521 ataataatcg atcgtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
     5581 atcaacaatg attagctgac agtccaccgg attagcgtga ctgtcatatt cacagctctg
     5641 ttttacatta ccaacccgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
     5701 gatcggatcc gttgatggcg tggcgctgac agataatgtt ttgtcgttac ttttcgtggt
     5761 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcattt gtttgctgct
     5821 ggctttaatc acgaagccat tatcatcggt ttcccaactc accccggcag aaggtaattc
     5881 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
     5941 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
     6001 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
     6061 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
     6121 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
     6181 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
     6241 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
     6301 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
     6361 cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc gccgggatcc tgtgagcgca
     6421 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacgt
     6481 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc ggccattgcg
     6541 ctaatgatac gcataaaatt ctctataccc gcaccaccag cggtaacgtc tctgctcccg
     6601 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
     6661 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
     6721 cttctattga tatggattaa taattcttaa cccaaaatgg gtagactccc tctattgtta
     6781 gcgcgctaaa tattcaatat ataaacattt atataacgat aaagaacagg gagtgagtta
     6841 tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa tcaaccgcga
     6901 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
     6961 cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc gtcaactgtg
     7021 gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg gtggaaactg
     7081 acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg cgcggtcgct
     7141 ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggta
     7201 cacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
     7261 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
     7321 cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg gtcaaacgcc
     7381 tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc gcgcaaattt
     7441 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
     7501 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
     7561 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg
     7621 atccgcgata taccgatacg gctgctggtc gtgaagatga atggctccct attcgcccgg
     7681 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
     7741 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
     7801 cacccaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat aacacagcga
     7861 aaacgccgca gtgggcttcg caaattaccg gtatcccggt agaccgtatc atcaaactgg
     7921 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
     7981 aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt acctattttg acgggcaatg
     8041 tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
     8101 gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacagatg
     8161 ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc aaagataaac
     8221 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt
     8281 ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtga
     8341 tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga
     8401 tgaccgttga gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca
     8461 ttttcctcca gcctgtcacc agcgaaaaat tcgaacgcaa accgatttac tggatcctga
     8521 gtgaagtcgc gaaacgtctt ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc
     8581 aggaacaatg gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt
     8641 cttacgacga actgaaaaaa tgggtatcta taagcgtaaa gatcccaatg gccattttgt
     8701 cgcctacaaa gcatttcgtg acgaccccga ggcaaatcca cttaaaacgc cttccggtaa
     8761 gattgaaatt tattccagca ggctggcgga aattgcccgt acctgggaac tggaaaaaga
     8821 tgaagtgata agcccattgc cagtttatgc ctcaaccttt gaaggctgga actcccctga
     8881 acgtagaacc ttcccactgc aactgtttgg tttccattac aaatcccgta ctcactcgac
     8941 ctacggcaat attgatctcc tgaaggctgc ctgccgtcag gaggtgtgga tcaaccctat
     9001 agatgcgcag aaacgtggga ttgccaacgg cgatatggtg cgggtgttta accatcgtgg
     9061 cgaagttcgg ctaccagcca aagtaacacc acgtattctc cctggagtta gcgctatggg
     9121 ccagggagcc tggcacgagg ccaatatgtc tggcgataaa atcgaccatg gcggctgtgt
     9181 gaatacgcta accactctgc gcccttcacc actggcgaag ggaaacccgc agcacactaa
     9241 tctggtcgag atcgaaaaaa tataacccac gacaaccata aattctggca tgacattttg
     9301 tttgaaaagc aataagtgag taatgatgaa aatccatacc acagaggcgc tcatgaaggc
     9361 tgaaatcagc cgtagaagtc tgatgaaaac ctccgcactt ggcagtcttg cgctggcaag
     9421 cagtgctttc actctgccat tttcccaaat ggtccgggcg gcagaggctc cggtagaaga
     9481 gaaagcggtc tggagttcct gcaccgttaa ctgcgggagc cgctgtctgt tacgtttgca
     9541 tgtgaaagat gacaccgtgt actgggtgga gtctgatacg acaggtgacg acgtctacgg
     9601 taaccatcag gttcgagcgt gtttacgcgg gcgctctatc cgccgacgga tgaatcatcc
     9661 tgataggttg aaatatccca tgaagcgcgt cggcaagcgc ggtgaaggta aatttgaacg
     9721 gataagttgg gacgaagccc tggataccat cagtgataat cttcggcgga tcctgaaaga
     9781 ttacggcaat gaggctgtac atgtcctgta cggaacaggc gtagatggcg gaaacatcac
     9841 caactcaaac gtcccgtacc gtctgatgaa ctcttgcggt ggttttctca gtcgctatgg
     9901 cagctacagt accgcacaga tcagtgccgc aatgagttat atgttcggtg ccaatgatgg
     9961 caacagcccg gatgatatcg ccaatacgaa actggtcgtt atgttcggaa ataacccggc
    10021 agaaacgcgg atgagcggcg gtggtgtcac ttactacgtc gagcaagccc gcgaacgttc
    10081 aaacgcacgc atgatcgtca tcgatccacg ttataacgac actgctgccg ggcgtgaaga
    10141 tgaatggctg cccattcgcc ctggcaccga tggcgcactg gcctgtgcga ttgcctgggt
    10201 actgattact gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga
    10261 tgaaaaaacg ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg
    10321 cgaagggcct gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc
    10381 ggcagaaaaa attatccagt tggcacgaga gatcggttca gcaaaacctg cttatatttg
    10441 tcagggttgg gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat
    10501 gctttccgtt ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg
    10561 tagctgggat ctgggggtag aatggttccc gatgcttgag aatcctgtta aaacgcagat
    10621 ttccgtcttt acatggacag atgccatcga ccatggtacg gaaatgaccg cgacccgtga
    10681 tggtgttcgt ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag
    10741 taacacattg atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga
    10801 cagcaagtgc gagatgattg ttggcattga ccacttcatg acggcctcgg ctaagtattg
    10861 cgatatcctg ttgcccgacc tgatgccaac agagcaagaa gaccttatct ctcatgaatc
    10921 tgcagggaat atgggctatg tgatcctcgc ccaacccgca acctcagcaa aatttgaacg
    10981 caaacccatc tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca
    11041 aacctttact gaaggtcgca gtcagcatga atgggtcaaa tatctccatg cgaaaacgaa
    11101 ggaacgtaac cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa
    11161 aaaatgcccg gaagaacact acgtcgcttt ccgcgcattc cgtgaagatc cacaggcaaa
    11221 cccgttgaaa acaccttcgg ggaaaatcga aatttattct gaacgactgg cgaagattgc
    11281 agatacctgg gaattgaaaa aagatgaaat tattcatccc cttcctgcgt ataccccagg
    11341 ttttgatggc tgggacgatc ccctgcggaa aacctatcca ctgcagttaa cgggcttcca
    11401 ttacaaagcg cgtacccact ccagctacgg caatattgat gtgttacagc aggcctgccc
    11461 acaagaggtg tggatcaacc ccattgatgc tcaggcacgc ggtatccgtc atggcgatac
    11521 cgtgcgggta tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat
    11581 tctgcctggc gttaccgcca tcgggcaagg tgcgtggctt aaagcggata tgtttggtga
    11641 ccgggtcgat cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc
    11701 aaagggaaac ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaag gagtagccca
    11761 tgaccacaca atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg
    11821 aactggcgtg caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg
    11881 aatacgctgg cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt
    11941 attatctctc catctcctgt aaccattgcg atgaccccgc atgtacaaaa gtctgcccca
    12001 gcggcgcaat gcataagcgt gaagatggct ttgtggtggt tgatgaagat gtttgtatcg
    12061 gctgccgcta ctgccacatg gcttgtccgt acggcgcacc acagtacaat gctgaaaaag
    12121 ggcacatgac gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat
    12181 gcgtcgaatc ctgcccgctg cgtgcacttg agttcggtcc aattgaagag ctgcgccaga
    12241 agcacggcac tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata
    12301 tcgttatcaa acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta
    12361 atccggagga agtgtaaaat gggaaatgga tggcatgaat ggccactggt tatctttacc
    12421 gtattgggtc aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc
    12481 aaaaatgacg cagaccgtca gcgtattgtc cgtggcatgt ttttcctctg gctgttgatg
    12541 ggcatcgggt ttattgcctc tgtcatgcac ctgggatcgc cactgcgggc gtttaactcg
    12601 ctgaatcgaa tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt
    12661 gccgttggcg gactatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg
    12721 aaactctggt tacttgtcag catggcgctc ggcgtcattt tcgtctggat gatgacctgc
    12781 gtgtatcaaa tcgacactgt gccaacctgg cataacggtt acactacgct ggctttcttc
    12841 ctgacggtat tgttgagcgg tccaatactt gctgccgcaa ttctgcgggc agcccgcgtt
    12901 acatttaata ccacaccatt tgccatcatt agcgttctgg cgctgatcgc ttgtgccggg
    12961 gtgattgtgc tgcaaggttt gtctctggcg tctattcact catccgtgca acaagccagc
    13021 gcactggtac cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt
    13081 cttggatgct ggctttgccc gctcattcgt cgccgtgaac ctcacgttgc cggacttatt
    13141 ctggggctaa tcctcatact tggcggtgag atgatcgggc gtgtgctctt ttatggcctg
    13201 cacatgacag taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat
    13261 ttcaggatgt taggaatgac ccatttttca cagcaagata atttttctgt cgcggcgcgc
    13321 gtgttgggcg cgctgtttta ttacgctcca gagagtgcgg aagccgcacc tctggttgcg
    13381 gtactcacca gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg
    13441 ctggtgacgg cttttcagac acagtgtgaa gagactcacg cccaggcctg gcagcgtttg
    13501 tttgtcggcc cgtgggcact gccgtctccg ccgtgggggt cggtctggct ggatcgcgaa
    13561 tctgtgctgt ttggcgattc aacattggca cttcgtcagt ggatgcgcga gaaaggcatt
    13621 cagtttgaaa tgaagcaaaa cgaaccggaa gatcattttg gttcactgct gttgatggct
    13681 gcgtggttgg cggagaatgg tcgccagaca gaatgcgaag aattgctggc atggcacctt
    13741 tttccgtggt caacacgttt tcttgatgtt tttatcgaaa aagcagaaca ccctttctac
    13801 cgtgcactgg gtgaactggc tcgcttaacg ctggcgcaat ggcaatcaca actgttaatt
    13861 cctgtcgcgg ttaaaccgct gtttcgatag gatcaccgta atattgccgg atggtgacga
    13921 ttacgcgcaa tccggcaata ataggttaca gtgtcacgtt tttttatctc ttaaagcacg
    13981 cactgctttt gcggctggcc tcttttgccg caaaatagtc gcccgtgttt cattgcccat
    14041 ttctgctcat gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc
    14101 ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcga
    14161 tgctgctact ggagtggttg ttcctcaata atgactccgg cagtctggtc aatgcagcga
    14221 caaacctttc cccctggcga cggttgctaa ctccggcgct cggcggactg gcggcgggtt
    14281 tgttgctgat gggctggcag aaatttaccc aacaacgccc tcatgcgccg accgattaca
    14341 tggaagcgtt gcaaaccgat ggacagttcg attacgcagc aagcctggtt aaatcgcttg
    14401 cctctctgct ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt
    14461 tagctgccct tgccgcctcc tgttttgccc aacgttttac gccacgccag gagtggaaat
    14521 tatggatcgc ctgtggggcc gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg
    14581 gcagtttatt tatagccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg
    14641 tgattatttc cgccgtcgtg gcattgctgg ttagcaatct gattaatcat agcgacgcgt
    14701 tactctacaa cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca
    14761 gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgtc
    14821 atcgtggatt tgtgagtctc aaacttgcgc cgccctggca actggcacta ggcgggttga
    14881 tcgtgggtct gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac
    14941 aatccttttt aaccgcccca ccactgttaa tgatcattgc cgggatcttc ctttgtaaac
    15001 tgtgtgccgt gctggcgagt agcggttccg gcgcacccgg tggtgtcttt acaccgacgc
    15061 tatttatcgg tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg
    15121 atggcgaaga aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa
    15181 ccacgcacgc gccgattatg tcgacgttga tgatatgtga aatgaccggg gagtatcagc
    15241 tactccccgg tttattgatt gcctgcgtca ttgcgtcggt aatttcgcgg acgttacacc
    15301 gtgactctat ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt
    15361 tctcgctgtt cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt
    15421 ttacccagca catcaatgat ttccgcataa tgcgccagtc ctgggttgat tcggttagcc
    15481 acccagccaa tgagcggcag cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg
    15541 ttaatgcaac cttcctgaat accgacaacc atcaacaccg gcaactgttc ctgcaccacc
    15601 cattcagaga gtggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc
    15661 acgacatgat cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg
    15721 attgggcaac tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct
    15781 tcataaggca gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc
    15841 ccttcgggtg tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc
    15901 tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct
    15961 gtaccggtaa taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat
    16021 aaaccaggaa aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga
    16081 tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa
    16141 agaaccgtta tacatcgcgt cttttaccag tgcagcgcct gccatcgtgc cctggttaga
    16201 aaactgagta ctctcaacgc tgatgtgctg actatacgca ggaagggcct gctgacggat
    16261 gctgtctgag atgaccggga agaggatatc tgccgcttta cttaacggtg agccaatcag
    16321 tattttttgt gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc
    16381 caccccggta atgatgtctt ttgccagcag atcgccgcgc aatgccgcct gacacaatga
    16441 gtccacggtt aacggttgtc catgtaacat cgagctcatg gattgattaa gacgcagctg
    16501 tgccagctca agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca
    16561 ataacagcgt ttcccatacg ggtcgacctg tgtgtggcct atttccacga gactactgct
    16621 gcctgcgtgt agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac
    16681 cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca
    16741 tgcgctgata tcatgctgaa tataaaccgg tacgccggta tgctgctcca gcgcctcgcc
    16801 gagcggcatc tcttttacat cctcgtagaa cggcatgcga tgtacaatac cattttccgt
    16861 atcaataatt cccggcaagg ttatggcaat cgaagttaga cgctcaagtt ttttctggtg
    16921 gcgaataaaa aactgatcga tatgggaaat gatacgatcc agcaatggcg agtcatcttt
    16981 taacgccagt tcctgcgact cttccaccac cagtttgctg ctcagatcgc gcagagcaag
    17041 gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac
    17101 cagccccacc gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag
    17161 gtgtgcttcg agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag
    17221 acgggaaaga tcgatacgcg agactggacc aagctgatca atcaggcgat aaaccgcgcc
    17281 cgcgttggtc tgctttattt gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata
    17341 ctccctatat tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt
    17401 gtcgtcaaat ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga
    17461 aatttccctc acctgagagc attcagcaga tgaatacgaa agttacgcgc agccggactt
    17521 tgttcatgat gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc
    17581 ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg aagcaggcaa aattgaaaca
    17641 cccagaccgg cggaaaccag accgatgatg gtcattgcct cgcccacctc ctgagtgatg
    17701 acgggcgtca aatggtaacg tcgcatcagc ccgagaatat cgtcatacag ccctgtcccg
    17761 acgtgcggat caaaaaagac gaagggttct ttcgccagtt cagccagcgt tacattcggg
    17821 ttatttgcca ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact
    17881 gcgtgttcaa gcgtctccgg taacgctgtg taacgcagca atcccatatc cagcgttcct
    17941 tcaatgagcg gagcgatttg ctcacgagtg ttcatttcgc gggtttgtaa atggacatcg
    18001 ggataatcac ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac
    18061 gaagtaaaac caatgcgcaa ctcccccgct tccccctgat gcagtctttc agcgcgagcg
    18121 gcagcgtcat ctaccataga caggatttgc cgactatctg caagaaactg ttttcctgct
    18181 gccgtcagca atacactgcg attagttcgt gccagcagtc gggcaccaat ttgttgctcc
    18241 agtgcctgaa tctgctgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc
    18301 ccgaaatgca gctcttccgc aacagcaaca aagtaacgca gatgacgaag ttcaatattc
    18361 atatttaaaa catcttattt gagattatta atatattaga cagaacaatt cgattttcct
    18421 aacctatgta taagcctgat ccacaggcat atttagcaag gatttcaagt gagccgtact
    18481 acaactgttg atggcgctcc ggcaagcgac actgacaagc aaagcatttc tcagccaaat
    18541 caatttatta aacgcggtac gccgcaattt atgcgcgtca ccctggcgct gttctctgcc
    18601 ggactggcaa catttgcact tctctattgt gtgcagccta tccttccggt gctttcgcag
    18661 gagtttggct taacgcccgc gaacagtagt atttcactgt ccatttccac ggcgatgttg
    18721 gctattggtt tgctgtttac tggcccccta tccgatgcca ttggtcgcaa accagtgatg
    18781 gtcacggcgc tactgttggc ctccatttgt acgttacttt cgacaatgat gaccagctgg
    18841 cacggcattt tgattatgcg cgccttgatt gggctttcgt taagtggcgt ggcagctgtt
    18901 ggcatgactt atcttagcga ggaaatccac cccagtttcg tggccttttc gatggggttg
    18961 tatatcagcg gcaactcaat tggcggcatg agcggacgct taattagtgg tgtcttcacg
    19021 gactttttca actggcgaat tgctctggcg gcaatcggtt gtttcgcgct agcctcggcg
    19081 ttgatgttct ggaaaatcct ccctgaatca cgccattttc gcccgacttc gctgcgacct
    19141 aaaacgttgt ttatcaactt tcgtctgcac tggcgtgacc ggggattacc gttattgttc
    19201 gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt ttaattacat cggatatcgg
    19261 ttgatgctct ccccctggca tgtcagtcag gccgtggttg gcttattatc gctggcttat
    19321 ttgaccggta catggagctc acccaaagcc ggaaccatga ccacccgcta cgggcgcggt
    19381 ccggtgatgt tgttttcgac gggggttatg ctgtttggtt tactgatgac cttattcagc
    19441 tcgctgtggc tgatctttgc cggaatgtta ctcttctcag caggattctt cgcagcccac
    19501 tcagttgcca gcagctggat tggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg
    19561 ctgtatctgt tcagttacta tctggggtcg agtattgccg ggacgctggg tggtgttttc
    19621 tggcataact atggctggaa cggcgtcggc gcatttattg ctctgatgct ggtcattgct
    19681 ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag cccggactgc
    19741 ggtaactaca cgtccggact tattgcttgc tcaaaccaac gttaatagcc atcctaaaat
    19801 agacgaagcg ccagccaatt cccgcagcgc gtctagcgtc atcaggatta taagtaccca
    19861 aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct
    19921 ttgtaagtag taaaatagtt aacccgatca agactactat tattggtagc taaatttccc
    19981 ttaagtcaca atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta
    20041 cacgctgaaa ccaaccactc acggaagtct gccattccca gggatatagt tatttcaacg
    20101 gccccgcagt ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta
    20161 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
    20221 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
    20281 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
    20341 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
    20401 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
    20461 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc aggtaacgtt
    20521 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
    20581 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
    20641 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
    20701 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
    20761 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
    20821 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
    20881 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
    20941 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acgccgccta
    21001 agggtaaagc ggataaggcg gtagcgctgc gttttgtgtc aaataaaggt ctttggcgct
    21061 atgacatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
    21121 atggggatgg ttggattgtt cctcccgcag ctgcaccatg ggacttcggc ttgattgtgc
    21181 tgcgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
    21241 ttactgccgc attaaaatcg gctggtcgta aagtgactca ggcgggatat cctgaagatc
    21301 atctcgatac gttatacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
    21361 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctttg atgttgcata
    21421 ccgatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
    21481 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
    21541 cgcaaaaata atgttcaaac gctgcccgac agtgcgggca gcgtgttcat caggcaagtt
    21601 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
    21661 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
    21721 atgcataagc gacagacaag tcgatccctt ttacggcctg agaaagcgca ctaaaggcag
    21781 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
    21841 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
    21901 cccattcaaa ctgcgccatg gttcacttcc agttcaggtt tacgcgcttt acgggtacct
    21961 gatttgatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
    22021 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
    22081 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
    22141 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
    22201 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
    22261 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
    22321 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
    22381 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
    22441 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
    22501 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
    22561 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
    22621 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
    22681 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
    22741 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
    22801 cattatttat tgctgttata tataacttat ttcgcataac acattgattt aattaaataa
    22861 ttaacaccgc aaaaagtgcc aaaaatcgca aaatgactac accagtgact acaccgttcg
    22921 gtgcactgta tgaaacaacg tggaacaaat agacacaaga aatatacagg cggatcatct
    22981 ttccaggggg aagcacacca attcatgagg ggcgttaatg tcgatatggg gatccccacc
    23041 taccggaatt tttttccggt taaacgttaa tcaggctggt gggctttacc tgttcggtag
    23101 gtgttatgat tatcgtaata cctttccccc aatggggtaa aagcatcaac cgtaacgaaa
    23161 gtcgttatgg ttgccactga taccccaaat ttgggtatca cgagtaagcc ccaaaatctg
    23221 ggtgttactc ttactaccca aactacgggt agtttccgta gtttcatggt cgagttgcag
    23281 atctgcaact cccccattaa ccacaggcga atttccgcct tcggtcattc cttaatcatc
    23341 tgcgtaatca taatgattat tcagcaagcg caattttgcg ctttgcttaa agttcagcga
    23401 gttaccgcgc cgacttcctc aaattgaggt ttccgaaaat atcagcgggt tagcgattaa
    23461 gctgaccatt aattaaactg cgaaaatgtc gtagttgtga ggggtgtagc caacccattt
    23521 ttgggccgtc gggaatatca accagttacc gccgcaaccg ctccggcttc ttccagtggt
    23581 acgtgatttt ctccttctcc cgatacagtt caacgcggcg attgtaggcc agcatttcaa
    23641 gaacgcggat ccgtatgtcg cgcatatccg catcattaag ctggatacca tcacggcgca
    23701 tcacctcagc aacaacacgc gcataatttt cggctgtcac gctgtccggc tgcgtggtct
    23761 gttcgtcagc ctgctggctg attccagaga cgcggcggat taatcgcagt atttcggctt
    23821 ctgtcatgct gctgacctca ttacaccccg ctaaattctt ccagtttctg gcggtggctg
    23881 tcgcttatat caaaagcaaa atcctcatgc tctgcctgga atgtaccaaa cgccatcagc
    23941 gccgctacgc tcgggtctat cttgttcggt gattttttct tgttcggctt gatattggcg
    24001 ttcgcgtcac tctgcatcac gacgttactc atcgaccagg acaacaccgg atcgccacga
    24061 tgcacaatca ccctgcggtt aacaaaaact tcgaacgatt tcgccgccgg actgaatctg
    24121 aggtaggttt gcgggaacgg ctccacctca aaaccagccc cctgtaattg cgtcctgaga
    24181 tgcgtggcgt tccacgtatc aaagcccacc agcctgatat taaatttctc cgcatcctgc
    24241 atgatgtcat ctctgatccg gtcgtaatca atgcagtcac ctggcgttgt gcgtatccag
    24301 cccgctttag cccactggcg atagacagcg cggtttttat tggcggggtt ctgtagctgg
    24361 aactccggca gataatgacg ggaaaccagc atgatatttt taccgaccgg aaaggcatag
    24421 cacacgctgg aaatatcgct ggttgatgat aagtccagcc ccgcgtaaca ctcctgcccg
    24481 tgtaaatctt cctccgtgaa cgttccggca cactcagccc atgcgccgtt acccatccac
    24541 ggggtagccc cctggcacca gatattaaat cgctttgtca tcatctccac ccactgcgac
    24601 ggaatacccc gcgctttctg gatggttgag gccagttttt cacgatcgac ggaaacatca
    24661 atattagggt tcgcctttat ccacatcgcc ggatcatcaa cctcgttttc gtcgtccagc
    24721 tcgtaaatca gtacaaaaat tgaatcgtta acctcttcgc cgtccaggat ctggcagcaa
    24781 taatcatagt gctgtttaca ggctgaaacg acgttgctgc ctgatgtggt aatggcaaat
    24841 aacagccctt cgggacgtgc gcccatcccc agctcaagcg cggaataaac gccgttatcg
    24901 gggtgcaagt ggtattcgtc cacgatggaa aggcttgggt ttgttccctc gatggttgcc
    24961 gcttttgctg ccagcggctt taacaggctg ttgcttttcg ggtgtatcac cttatgcgcc
    25021 tgaatattca cccgcctgcg taacggtcgg gataaaaggc acatctgacg cgcatcatca
    25081 aagacgatcc gcgcctggtc acggctcacc gctgcggtgt agatatcctg ctgaccgttc
    25141 tccataatca gaaaccagtt agccagaatc gcggcggtcg tggatttcgc atttttgcgc
    25201 ggcacttcga taaaggcgct cgtgtatttg cgccgtccgg tggccttaac cttaaagccg
    25261 aggatgcacg caaaggcgaa ctgctgccac ggctccagct caatgggtct gccacgcatc
    25321 ggtcctttta cgtgcgggca caccctggaa aaggcaataa accgctccac aacctcacga
    25381 tcgaacgtgt aaagggggct tttaaggtcc gaaaagtacc gtttaacggc ctgtttcagc
    25441 cgtttacagg ccggaatttt gcccgttttt acgtcttctg cgtacttatt ccaggcggtc
    25501 aagctcgtcc tcttcttctg tttccggtgg gtttttacgg cggcttatcg ggtcaaaacc
    25561 gagcaaggag gccattttta tcatcaccct ttcagcgtct gatttcgcgc ttaatgcggg
    25621 gtttctgctc tcgccgccct gactgttaac aatgctgaac ccgcgcgccg caaggtctgc
    25681 gacggctttc cggtaaatgg agtagttgac acaatacagt tccagattgc tccagtcggc
    25741 gggggtcagg tctccccgtt ccgcaagctg ccgcgatttt tcccgccact gcttcacggc
    25801 gatgtcatcc aggtaggcgg gggctttcgg tggtcttgcc atgcttattt ttcgtcagat
    25861 tatttttcaa aaaattcccg tgcataaaaa tttgaggagg cgttcggtgc ccggcggggt
    25921 cgggtttgtc ctgaaaacgc cccccacccc gccatacagc ctcatcagcg attgcggaaa
    25981 cattccataa cctcgcggtc acggtcggtt aatcgcttcg ctgtgatgcg ttctgcgcgt
    26041 cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc atgcgtcacg ctgccttata
    26101 agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgtcataca tccagttatt
    26161 gcggttagcg gcccgttctt catcttcacg aaatccacct gcggcacgct tgctttttgt
    26221 tgccggatca agccatttcg ttttctggtt atgacacgcc tgacacaatg gctgatggtt
    26281 ccattcgggc cagaagagaa catcatcgcc gccatcgatg gggataatgt gatccaccac
    26341 cacggcgggc gtatatatcc ccttctcaag gcatcgcacg cataacgggt ttttactcag
    26401 atacatggcg cggtatttgt cccactgtct ggagtaccca cgcgcgcggc ggtgtcctcg
    26461 tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc tcacacttac cggacttaac
    26521 gcgcctgtta cagcctggtt ctgtacatcg tcttaatggt tgccacggca tcagtacacc
    26581 cccacatcac ggtaagccgt ccagagtgcg cctatcgtca tgggtacgcg cgtttttgcg
    26641 ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat gcagccaatc
    26701 tttatggctg gcgtgaactc cagcccgtca tcaaagcgcc tgcctatgtg tatctggcac
    26761 gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc gccatcgatg
    26821 cggcaatgga gtttcacttc ttccagggtg ataagttctg tcatttttcc gcgccctcac
    26881 gacaaagaat ttcaaggcgt gtcctggcgg catctggcag cggttgcccg atgatattca
    26941 gtacacgccc cgccagcggc cccgtattga cctttatccg actggtggcg ttgatgtcct
    27001 tccggtagcg tatccatatc cttacagttc cagtcgccag ctctgcacct gatgaaatgg
    27061 cctccttgct gctgatcatg ttcacgcttg cccagagtgt gtgaccgtcc agccacgttt
    27121 cgagtatttc acccgtcatg gctctggtct gtttcagggt ctgaatcgtt accctgtcac
    27181 gcagtcgccc tatgttcatt ccggtttttc tccctcgctg atttttactt cctgtttcca
    27241 tgcctggctg aactcatcac caccatcacg cggtgacagt ccttcacgtt cgcgggcttc
    27301 gttcggacac ataacgccgg atttgatgcc gcgctcatag gtggcaaagc gttcgccagg
    27361 tgtggcccgt aacaggtccg cgctgtcaaa ctccacctga taccggatac caggtacagg
    27421 cgaggccacc agcagggcgt tttttatctg ctgctcaaag ttcgccagcc acgggcgcat
    27481 cgtcatggtg agaaatgcgc ggcttgcctc gctgaaattg ctgtaggtgc tgttgctgta
    27541 ttcctgcaaa aaaatcggcg agacgttgaa cattctggcg atgtcttcaa tggtgaagcg
    27601 acgggaggcc agccattcag catcctgatt actcatgccc agctgctgat agctcatacc
    27661 cccttcaagg atgggcgttt ttccggcgtt tctggcccct ttgtagcgtt ccagtgcggc
    27721 taatgcctgt ttgcccttca cgccgtccag ccattcgcct gacgtgataa cccctgccgc
    27781 catcatgcca tcacgcatca cgctcgcgcc gtggcgttgt tgggccagcc caagccccag
    27841 cgattcgcgg cagatggtta caggtgagcg ccccataaaa ccgtcatccg tggagtaacg
    27901 aaggtggaga acctcccacg gtaaatagtt gcgggtgttt ccggtgtaag cgtcagtgat
    27961 gcagtagcgc cagttgtgtt ctcctgtctg ctccatgttc accgactgcg gcgggtaagg
    28021 atgcaaagcc gccggaaaac catcacgccc ccactgaatc accgcgtaag cattaccgtt
    28081 taacaggcag tggcggatca tcattcgctt aaactggtag ggagtctgcc acgcgttcgg
    28141 gcgctcgttg aggatgtgat cgaccggatg agaatcaagc cactcgcggg cctccttccc
    28201 cttctcattg cgtaccaggt acaggtaaca cggcatggta gccaccgcct cagagatgac
    28261 cgtcacggcg ttcatgacgg cgggcagtga ttccgctgtc cccgatgata cgtactcacc
    28321 cgcccccgtg ttcgatgtgc cagccagcgc cataaactca tcaagcgtca tgctgcgctg
    28381 ctcttttttt cttctgaaag gccacatatc acacccccgc taaatccgcc caccagcggc
    28441 gattatccgc acgcggcatt ttttcggggt gctgctcgta cagggagcgg cgggccagct
    28501 ccacgccgga atcagggtaa gccggtacgg atgtgacggt aatttcgtac agttccgcaa
    28561 ccagcacggt gcgcacgcat ggatctgttg tggtatccca tacatcctta cgggaacgaa
    28621 agccaaagct catgccggat atatcaccac gcttaaccag ttcgataacg tcgcgccctg
    28681 tactggtatc cggtggggtc agttcaaagc gtaaccctgt ttcatcctct tccagtttca
    28741 gcgtgccgga acgggtgcgc cccagtaaca tactgtggtc atgctcatac aggccgcgaa
    28801 cgtcattacc cgccgcaagc cattcagtaa acgccccccg ctggaatttt tcgtaaaact
    28861 caccccataa cagttctgaa agcttatccc agcgaacaac gtagcctgta agcgtgttgc
    28921 tgccgctggt ggtgatttcc gatgaccgga tttccatact cttcataatt ttttcaccca
    28981 taaaacacta aaggggcttt taagcccctt ctgtatgctg ttaatcgtcg tcctgtggca
    29041 gttccagaat cttgatcgcg ttcgaatcca ccacgccacc gcctaaatat ttctgcgtga
    29101 aaattttgat gaagcccggc tctgtgaggt tgtccggtct ggtgcgaaca cctgtttcgt
    29161 gatcaacaat gtagtaaccg cgtttgaagt cacccagggc aataacgtta tcaggcataa
    29221 actccagata ttcgaccgga aggcccagca acgtatcagg atcacccgcc tgtaaacggt
    29281 cgcgccagat ataatcaccg ttcgcgttct tcaccttctg gagttttgcc gccgtcgtgg
    29341 agttaaccac ccagaccgcg tttttgcggt attttttacg taatgcaaat ttcaggtcga
    29401 tcagcgggtc cgcagatgtc cacgccagag attcggaagg tttaattacc tgtaacgtac
    29461 caaaatcacg ctctttgtcg ttcttctctg cacggggtac ggataaaaag ccttttgctt
    29521 ttttgtcacc atcgcccaca accagatcgc tttcttcggt ttccgtgaag gtgtcgccaa
    29581 tctcacccgt cagccatgaa aggatgtcca catcggaaaa atccacgatt tcctgtgtgg
    29641 tgcgcgggta cgcatagacc ggatacagct taatgctcac ctcgttaatc tgcggggtgc
    29701 tggtctgttc gcgtgcctta ccctcttcac cgtggttaac ggtcgcaccg ccagcggaaa
    29761 caagctgctt aaactcgttg ctgctgattt tcttcacggt acagatgcgg cgcatggtgg
    29821 attcatccgc cagcattcgc atgatttcgg tgttcagttc agggataacg gtataaccac
    29881 catcagcggg aacgcctgta cttaatgcgc gggtttcacc tgtcagaatg tagttgcgta
    29941 gttctgcggg gtcagtggtc tgactgcttt tacctggctt gctgcgctct tcgtctgcaa
    30001 tggcttcaag gcgggaaatg tctttatcga gggattcagc tttagcgcgt aattcgtcaa
    30061 attttgcgcc ctcagcatcg ttaagactgc ggttttcttt ttccgcgttc tccagcatgt
    30121 cgcgcatctg atttttaatg gcggttttct gctggcgtaa ttcgattatt ctcggcataa
    30181 aaaaagtcct ggggttaagt aaggaactcc aggacgcggc aaacactcaa ccgtttttca
    30241 taaggaaatc agtaatcgca ccgatcgttt tcccgcctgg taatgaatat tggcgagcac
    30301 attaacaggc gggaaagtgg ccccagcgtc ctggcaccac gggcgagaat actcatgatt
    30361 cagttcgggt aaaatatgcc gatcctgtca gtgaacaacg tggaacaacc acgaacaaat
    30421 aatttacaaa aaatgacaaa aagccggatt gctccggctg tatgcgtgct cagtccctga
    30481 tccctttcag aaattctatc aatgcatcta tctgttcagg atttaccgcc agcatttcac
    30541 cggatagagc acatctaaca aaaccatgct gatccttttc aatcagtgcg cccgtctcca
    30601 ggaatgcgcg gtaatcattg atgctcatcg tctccatgtt gtcagcatgg tatttatcac
    30661 gctgttttaa tatctcatca aatttcatcg gcattgtttt ttcctctgtt gtgtctgttt
    30721 gtttcagata gtaactatgc cagaccgtga cgaaaacccg gtaatgcgcc ataccgtttc
    30781 aactggtgca aaaaaagccg gatttctccg gctgtgtgat tagctgtcct ggtaattgcg
    30841 ccatatttca tcaccagcac catctatccc catttcggca taagtgcgat cgactgcctt
    30901 tttcaggtct ccgtaattat ccggtggctc cggtggcctc tgtgccttcc tgcaacattc
    30961 cagccgtcgc atcgtaacct gatgccgttc cttgtctgtc tccaccagca gcatgacttc
    31021 accccatcgc gccgccgccc tccggtaaaa gcctttcgcc tcgagttctt ccgctatgcg
    31081 gtcatgtacc atcgccaccc cctcagaacg gaataccgtc accgtaaggg tcatcgcctc
    31141 ccgctggtgg ctgattaccc tgtgtgcctg tggttttgcg tctgttcccg ccaggaagtg
    31201 ccgcacgggc actgattacg ctgtctgcaa taacctgata accctgccgc gtttccccat
    31261 tctgtccggt ccactggctg acctgcatcg tgccggatac gctggcaacg tcgccttttt
    31321 gatgtttagc caggaagtcg gcctgcttac caaatgcgat gaccgatagc cataacgtcg
    31381 cctgcccatc ctgcgcctga ctacatggca gcgataccgc catacgcgcc agcgtcatcg
    31441 gtgtaccctt gctggtctgt tttacctgcg ggtcgtccac cagccgcccg taagctgcta
    31501 tctgtgctgt catgattcca cctctccggt tttaacgttg atggttgtta cctgttccgc
    31561 ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc
    31621 gcttatcagt tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct
    31681 gattgccacc tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct
    31741 gtctctgcgt tttttgagtg atttattaaa tttccctgaa gtgtgcatat ttatttttac
    31801 cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg
    31861 ccctcatacc agtaatggcg cggctttcag ctgggtagag tgcttttatc cactatctac
    31921 cccgtgtcta cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt
    31981 agacagtaaa aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt
    32041 actcaggtag acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc
    32101 tgttgttatt gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc
    32161 gtaaactttc gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct
    32221 cctgcctgcg tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta
    32281 cttcactgaa tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag
    32341 gaactggcaa aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc
    32401 gtcacgctgt tccagtagta aagcccgtgc cttctcaggg tcggtaaagt tcgccagcaa
    32461 tcggcggata atgacaggga tttcagccgc gatcttttcc ggtagctccc tgtctttttc
    32521 ggcctcactg acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc
    32581 ccgttcggtg aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc
    32641 cgtgaaacgc ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt
    32701 gatgcccgtt ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc
    32761 gacaacctgc gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt
    32821 ctgtttccct gccagaaggc tggctatgtg cgtgaatgta cttttaccgc tcccgccgtc
    32881 tccggtggcc tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc
    32941 ggcacatata cgcatcatct tgcgcgggtc ttttccggct gcgtgctcaa gccatttatg
    33001 aaagtttggc gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc
    33061 gttgtgcgtg gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc
    33121 aagcgcacca ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat
    33181 aacttttaac gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc
    33241 tgaatagatc gccaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc
    33301 gccgcgccat acgtgaacga tttcactttc aggatgtacg caaacgccat caaagcgatc
    33361 ggcaagcagc tgcgcgcgct cactgtccgc catctgcgaa agttgcgcct tttgctttac
    33421 cggaagctca atgaccagac catcagaaag attctggcgc tcacgagcca gatattcgcg
    33481 ccagttctcc accttctgac cgtgcatacc atcagggtaa aaatttgcat cctgtacgcc
    33541 tgccgccgcc agcttctgac caatcgcctt tatcattact ggcgcaagat atccggccct
    33601 gaatatgcgc acggattttc tgccttccgg cacaatttgc agcttatcca gttcggataa
    33661 ctgctgctcc ccaagccaca caggaggctc attatctccg gccatacgcg cgtcatgttc
    33721 ctgccattgt tttgcgtgtg accaggcatc actacccgca aaaataatga cttctgtttc
    33781 tttgtgtttt atgccgcgtg actgctgttt tacgttcggt gccagtttca ttttttaccc
    33841 ctgaatacgt taagcatctt ttttatttcc tgaatattgg cgcgtgcttt cttcctgctg
    33901 gttggtttac tgcggggcgc tgcctgtacc agagaaaaat cacgccgaaa ctgataaaca
    33961 ggcatcacgc aatcatattc gta
//
LOCUS       DAZKGS010000033        33279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKGS010000033 DAZKGS010000000
VERSION     DAZKGS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..1174)
                     /locus_tag="ACHOQY_001608"
     CDS             complement(5..1174)
                     /locus_tag="ACHOQY_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9677735.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKAQKLAQKLSTKNSFES
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWQELDLEKGLWEIPAERMKMRRPHIVP
                     LSRQVIRLFQQLKPITGHYPYIFIGRNNRKKPISKESINQVIELLGFKGRITGHGFRH
                     TMSTILHEAGFNSAWIEMQLAHVDKNSIRGVYNHAQYLNGRSRMLQFYADKLYPN"
     gene            complement(1337..1431)
                     /locus_tag="ACHOQY_001609"
     tRNA            complement(1337..1431)
                     /locus_tag="ACHOQY_001609"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1395..1397),aa:Sec,seq:tca)
     gene            1724..3106
                     /locus_tag="ACHOQY_001610"
     CDS             1724..3106
                     /locus_tag="ACHOQY_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9677736.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3116..5434
                     /gene="yicI"
                     /locus_tag="ACHOQY_001611"
     CDS             3116..5434
                     /gene="yicI"
                     /locus_tag="ACHOQY_001611"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9677737.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5487..7196)
                     /locus_tag="ACHOQY_001612"
     CDS             complement(5487..7196)
                     /locus_tag="ACHOQY_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9677738.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(7317..8708)
                     /gene="xanP"
                     /locus_tag="ACHOQY_001613"
     CDS             complement(7317..8708)
                     /gene="xanP"
                     /locus_tag="ACHOQY_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9677739.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8988..10193
                     /gene="gltS"
                     /locus_tag="ACHOQY_001614"
     CDS             8988..10193
                     /gene="gltS"
                     /locus_tag="ACHOQY_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9677740.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10196..11062
                     /locus_tag="ACHOQY_001615"
     CDS             10196..11062
                     /locus_tag="ACHOQY_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677741.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11047..13128)
                     /gene="recG"
                     /locus_tag="ACHOQY_001616"
     CDS             complement(11047..13128)
                     /gene="recG"
                     /locus_tag="ACHOQY_001616"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9677742.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13134..13823)
                     /gene="trmH"
                     /locus_tag="ACHOQY_001617"
     CDS             complement(13134..13823)
                     /gene="trmH"
                     /locus_tag="ACHOQY_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9677743.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13830..15938)
                     /gene="spoT"
                     /locus_tag="ACHOQY_001618"
     CDS             complement(13830..15938)
                     /gene="spoT"
                     /locus_tag="ACHOQY_001618"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9677744.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15957..16232)
                     /gene="rpoZ"
                     /locus_tag="ACHOQY_001619"
     CDS             complement(15957..16232)
                     /gene="rpoZ"
                     /locus_tag="ACHOQY_001619"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9677745.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16287..16910)
                     /gene="gmk"
                     /locus_tag="ACHOQY_001620"
     CDS             complement(16287..16910)
                     /gene="gmk"
                     /locus_tag="ACHOQY_001620"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9677746.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17168..18850
                     /gene="ligB"
                     /locus_tag="ACHOQY_001621"
     CDS             17168..18850
                     /gene="ligB"
                     /locus_tag="ACHOQY_001621"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9677747.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18847..19464)
                     /locus_tag="ACHOQY_001622"
     CDS             complement(18847..19464)
                     /locus_tag="ACHOQY_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9677748.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19756..20580)
                     /gene="dinD"
                     /locus_tag="ACHOQY_001623"
     CDS             complement(19756..20580)
                     /gene="dinD"
                     /locus_tag="ACHOQY_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP9677749.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20801..21664)
                     /locus_tag="ACHOQY_001624"
     CDS             complement(20801..21664)
                     /locus_tag="ACHOQY_001624"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9677750.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21791..22507
                     /gene="rph"
                     /locus_tag="ACHOQY_001625"
     CDS             21791..22507
                     /gene="rph"
                     /locus_tag="ACHOQY_001625"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9677751.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22573..23214
                     /gene="pyrE"
                     /locus_tag="ACHOQY_001626"
     CDS             22573..23214
                     /gene="pyrE"
                     /locus_tag="ACHOQY_001626"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9677752.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23251..23847)
                     /gene="slmA"
                     /locus_tag="ACHOQY_001627"
     CDS             complement(23251..23847)
                     /gene="slmA"
                     /locus_tag="ACHOQY_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9677753.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23954..24412)
                     /gene="dut"
                     /locus_tag="ACHOQY_001628"
     CDS             complement(23954..24412)
                     /gene="dut"
                     /locus_tag="ACHOQY_001628"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9677754.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24390..25610)
                     /gene="coaBC"
                     /locus_tag="ACHOQY_001629"
     CDS             complement(24390..25610)
                     /gene="coaBC"
                     /locus_tag="ACHOQY_001629"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9677755.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25782..26450
                     /gene="radC"
                     /locus_tag="ACHOQY_001630"
     CDS             25782..26450
                     /gene="radC"
                     /locus_tag="ACHOQY_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9677756.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26667..26903
                     /gene="rpmB"
                     /locus_tag="ACHOQY_001631"
     CDS             26667..26903
                     /gene="rpmB"
                     /locus_tag="ACHOQY_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9677757.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26924..27091
                     /gene="rpmG"
                     /locus_tag="ACHOQY_001632"
     CDS             26924..27091
                     /gene="rpmG"
                     /locus_tag="ACHOQY_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9677758.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27189..27998
                     /gene="mutM"
                     /locus_tag="ACHOQY_001633"
     CDS             27189..27998
                     /gene="mutM"
                     /locus_tag="ACHOQY_001633"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9677759.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKYAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28037..28516)
                     /gene="coaD"
                     /locus_tag="ACHOQY_001634"
     CDS             complement(28037..28516)
                     /gene="coaD"
                     /locus_tag="ACHOQY_001634"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9677760.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28524..29801)
                     /gene="waaA"
                     /locus_tag="ACHOQY_001635"
     CDS             complement(28524..29801)
                     /gene="waaA"
                     /locus_tag="ACHOQY_001635"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9677761.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30214..31272
                     /gene="rfaQ"
                     /locus_tag="ACHOQY_001636"
     CDS             30214..31272
                     /gene="rfaQ"
                     /locus_tag="ACHOQY_001636"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9677762.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31269..32393
                     /locus_tag="ACHOQY_001637"
     CDS             31269..32393
                     /locus_tag="ACHOQY_001637"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9677763.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            32386..33183
                     /gene="rfaP"
                     /locus_tag="ACHOQY_001638"
     CDS             32386..33183
                     /gene="rfaP"
                     /locus_tag="ACHOQY_001638"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9677764.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7857 a         8234 c         8858 g         8330 t
ORIGIN      
        1 tttatcagtt tgggtatagt ttatctgcat aaaactgaag catacgagat cgaccattaa
       61 gatattgtgc atgattgtat acaccgcgaa tgctgttctt atcaacgtgg gcaagttgca
      121 tttcaatcca tgcactatta aaaccggctt cgtgtagtat ggttgacata gtgtgtctga
      181 aaccatggcc agtgattctc cccttaaatc ccaataactc aatcacttgg tttatgctct
      241 ccttacttat aggctttttt cggttatttc gtccaatgaa aatgtaagga tagtgaccag
      301 tgatcggttt tagttgttga aatagcctta taacctgcct tgaaagcggg actatgtgtg
      361 gcctacgcat cttcatacgc tctgctggta tctcccatag ccctttttct aaatcaagct
      421 cttgccatgt agcaaagcgc atctcttgtg ttcgaacacc tgtaagcatc acaatcttgg
      481 ttgcattttt agtgattatg ctgccagtgt atgcctcaag atctctgaca aaatgtggga
      541 gttcctctgc tgatagaaaa ggatgatgtt tttgcttcgg cactgctaat gcgatagcta
      601 aatcaggtgc tggattgtat tcagcgcggc cagtgatgat cgcgtagcga aacacttctc
      661 cgcaccgttg gcgtactttt cttgttttct ccaacgctcc acgtttttct atgcgtttaa
      721 gtacttcaag cagttctagg ggtttaattt cagctattgg tcgtttacca atgaaaggaa
      781 aaacatcctg ttcaaatgtt ttaataattt cttctcgata agctaaggtc caacgatctg
      841 cttttgctgc atgccattct ctgctaacgc tctcaaaaga gtttttagtg gataatttct
      901 gagcaagttt ctgagcttta cgttcttcta ccgggtcaat gccattagca acctgcttac
      961 gggcgatatc acgcttttca cgtgcttctg cgaggcttac taaatcgtag ctgccaaatg
     1021 acattaaacg cgctttccct gcaaaccgga aacggaaacg ccagcctttc gagccatcag
     1081 gattgataag caatgacaga ccttgtccat cgttcaatgt gtatggtttg tcttgtggtt
     1141 ttgcacgttt gatctgtata tcggttagag ccatgtgtat aactgaaaac tgcgtataaa
     1201 aaatctatac acattactat acatattttt cgcggattca gggagatgat ctcggactat
     1261 tacggataag ctgattattc atcgcattga atataaagga ttttatggag tgcctcggaa
     1321 ctggctggat gaggtttggc ggaagatcac aggagtcgaa cctgcccggg accgctggcg
     1381 gccccaactg gatttgaagt ccagccgcct caccggagac gacgatcttc cgcgcctcga
     1441 ttgctacatg gaggcggggc gcattatagc tacttccttg agtttctaca tcccccagat
     1501 cagttttttc cgcctaatac acgcagcgta ttatcacgaa agcattatag ttcatataaa
     1561 acctatattt tacaatgata tatgagcgaa attaacgcta ccacgatcac ataaaacaag
     1621 aatcataaat taataaccag atatcggaat attcgctctc acagggatgg tttacaaaat
     1681 gcgttatcgg tctcagcacc cctatagcat caaggaaaag cagatgaaga gtgaagtgtt
     1741 gtccgttaaa gagaaaattg gttatggcat gggagacgcc gccagccaca ttattttcga
     1801 taacgtaatg ttatatatga tgttctttta taccgatatt tttggcattc ctgccggttt
     1861 tgtcggaacc atgtttttgg tcgctcgtgc actggatgcg atttccgatc cttgcatggg
     1921 gttgttggcc gatcgaacgc gctctcgctg gggtaaattt cgtccgtggg tactgtttgg
     1981 cgcactgcca ttcgggatcg tctgtgtact ggcctatagc acgccagatc tcagtatgaa
     2041 cggcaaaatg atctatgcag caattactta caccctactt accttacttt ataccgtcgt
     2101 caatatccct tactgcgcat tgggtggtgt aatcaccaat gacccgactc agcgtatctc
     2161 gctgcaatcc tggcgttttg tgctggcgac cgcgggaggc atgctttcta ctgttctgat
     2221 gatgccactg gttaatttaa ttggcggtga taataaacca ctcggtttcc agggcggtat
     2281 cgcggtcctt tccgtggtgg cattcatgat gctggcattc tgtttcttca ccactaaaga
     2341 acgcgttgaa gcaccaccta caacaacgtc tatgcgggaa gatttacgtg atatctggca
     2401 aaacgaccag tggcggattg tcggtttact aaccattttc aatatcctgg cggtgtgcgt
     2461 acgcggtggg gcgatgatgt attacgtcac atggattttg ggcacgccgg aagtgtttgt
     2521 cgcttttctc accacttatt gcgtgggtaa cctgattggt tccgcactgg caaaacctct
     2581 gaccgactgg aaatgtaaag tcactatctt ctggtggacg aacgccctgc tggcagtgat
     2641 tagcctcgcg atgttctttg ttcccatgca ggccagcatc actatgtttg tcttcatctt
     2701 cgtgattggt gtgttgcatc aactggtgac acctatccag tgggtaatga tgtccgatac
     2761 cgtcgactac ggcgagtggt gcaatggtaa acgcctgacc gggatcagtt ttgctggcac
     2821 gctgtttgtg ctcaaactgg ggttggcctt cggcggcgct cttatcggct ggatgctggc
     2881 ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc gccacgatta gcatcattat
     2941 tgcgctattc acgattgttc cggcgatctg ttatttgctg agcgcgatta tcgctaaacg
     3001 ctactactca ctcacgacgc acaatctgaa aaccgttatg gaacagctgg ctcagggtaa
     3061 acgccgttgc cagcaacaat tcacctctca agaagtgcag aactaaggaa cggcaatgaa
     3121 aattagcgat ggaaactggt tgattcaacc tggcctcaat ttgattcacc cgcttcaggt
     3181 attcgaggtt gaacagcagg gtaatgaaat ggtggtctat gctgcccccc gtgatgtgcg
     3241 tgaacgtacc tggcagcttg atacgccttt atttacgctg cgctttttct ccccacagga
     3301 aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg ctgaataacg gtcctcatta
     3361 tccgctcaat attttgcagg acgtgaaggt cacaatcgaa aacacagaac gttatgctga
     3421 gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt cactggattt
     3481 tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa aataatggct acgtgcagga
     3541 cacgaataat caacgcaatt atatgtttga gcggcttgat cttggcgttg gcgaaacagt
     3601 ttacggtctg ggagagcgct ttactgccct ggtgcgcaat ggccagacgg tagagacctg
     3661 gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa aatatcccgt tctacatgac
     3721 taaccgtggt tatggggtac tggtcaatca tccccagtgt gtctcttttg aagtgggatc
     3781 ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa tatctcgaat actttgttat
     3841 cgacggcccg acgccgaaag cggtacttga tcgttatacc cgctttactg gtcgtccggc
     3901 gctgccgccc gcgtggtcct tcggcctgtg gctaaccact tcatttacca ccaactacga
     3961 cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa cgcaatctgc cgctgcatgt
     4021 tttccacttt gactgtttct ggatgaaagc cttccagtgg tgcgattttg agtgggaccc
     4081 gctgactttc cctgacccgg aagggatgat ccgccgcctg aaagcgaaag ggctgaaaat
     4141 ctgcgtctgg attaacccct atatcggtca aaaatccccc gtctttaaag agttacaaga
     4201 gaaaggctat ttactcaaac gcccggacgg ttcgctatgg cagtgggata aatggcagcc
     4261 aggtctggcg atttatgact ttaccaatcc ggatgcctgc aaatggtacg ccgacaaact
     4321 gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc gactttggcg aacgtatccc
     4381 aactgatgtt cagtggtttg acggttccga tccgcagaaa atgcataacc attatgcata
     4441 catctacaac gaactggtgt ggaacgtgct caaggacacc gttggtgagg aagaagctgt
     4501 cttgtttgcc cgctcggcct ccgtcggtgc gcagaaattc ccggtacact ggggtggcga
     4561 ttgttacgct aactacgaat caatggcgga aagcctgcgc ggtggtttgt ctattggcct
     4621 ttcaggtttt ggcttctgga gccacgatat cggcggcttt gaaaataccg ctccggcgca
     4681 cgtttacaaa cgctggtgcg cgtttggttt gctctccagc catagccgtt tacacggtag
     4741 caaatcttat cgtgtgccgt gggcctacga tgatgagtcc tgtgatgtag tgcgcttctt
     4801 cacgcaactg aaatgccgca tgatgccgta tctgtatcgt gaagctgcgc gtgcgaacgc
     4861 gcggggtacg ccgatgatgc gggccatgat gatggagttc ccggacgatc cggcttgtga
     4921 ttaccttgac cgtcaataca tgttaggcaa caacgtgatg gttgcgccgg tgttcactga
     4981 agcgggcgat gtgcagttct acctgccgga aggtcgctgg acacacctgt ggcacaacga
     5041 tgaactcgac ggtagtcgct ggcataaaca gcagcacggc ttcctgagtc tgcccgttta
     5101 tgtgcgtgat aacactctac tggcgctggg caacaacgat caacgtcccg attacgtgtg
     5161 gcacgaaggc acggcattcc acctctttaa tctgcaagac gggcatgaag ccgtctgtga
     5221 agtgcccgct gctgacggat cggtgatctt tactttaaaa gcagcacgta ctggcaacac
     5281 gattactgtg actggtgcgg gcgaggcgaa gaactggaca ctgtgcctgc gcaatgttgt
     5341 gaaagtaaat ggtctgcaag acggttcgca ggctgaaagt gagcaggggc tggtggtgaa
     5401 gcctcaaggg aatgcgctga caattacgtt gtaatctatt gatggaatgc cgggtacgtc
     5461 tgagtcgtac ccggcgagat tgatgtttac tgcaccagct ctgccgacgt aacttcaccg
     5521 ctggaaacga tgccagcatt catggtttga gtcacttgct gctttgcggc gttcacggca
     5581 tgcaattgcc cgctaaccgt aggtttcagc ggggcattgg cctgaatatc tccactggcg
     5641 gtaagctgaa tattgccgtc gccagtcaac ggtaattcag gccatcccca ttgttgcaaa
     5701 atattcaccg gcacaccgcg tccattcagg ctgatatggg tctgacgttg tggcgtttgt
     5761 gaaacactgg cagtggcttc cagaatgcct ttttcagtaa atgcactcag ttcgctgata
     5821 ttcaccgtgc tgctgttggc ggtcagcgcc agcgacgggc gacgaacatc aacacgattg
     5881 aatgtcgcgg cggcggcatt cagattcgcc gagccactcc agacgcccca tttatgatcg
     5941 gtaaccagcg tcaggttggc accgtaacca tcgagcgcgg tgagctgcca cgggaagtca
     6001 gggtcgatat caatgatcag attgcggctg gcgctaaatc tcttcagttg caggctgttt
     6061 aaccaaccgg gtgtcgtttc catccacaac tgttgccagt ttttcggcaa ggtatattcc
     6121 agcccggcaa ttgccacatc atcaaggatc aacgttttcc cgtcacgcag ccagttccct
     6181 gacgttctga ccataccccc ttcccagcgg ctggtgaact ggcgcagcgc tacgccctgc
     6241 ggggaaaatt ccgcgtttat aatcgggtca aataaatgca gcgaaccata aatgaactcg
     6301 ctagcgttca tcgacagttt gccttcctgt gtctgccagt catctttact gaaggtcatg
     6361 ttgcgcaagc tgagatcgag gtcggtcacc gcccagtccg gaccttgcaa acgagcatcg
     6421 atcacttcca ggcgaccaat ctgcaacgac gggacagagc gtaatggcgc aaagaagtcg
     6481 gttagcgatt tttcgctttg tagacgtata tccgccatgc gcaggttttc cacttgccag
     6541 ctgccgtcgg cgttacgctg cgcgtttccg gttaatgtcc cgcgggcgat gtcggcaccc
     6601 aggttagtca gcgtaacgcg atcgttatca atactgcctt caatcagtac attggtggca
     6661 ggaacatcat tgagcgaaag cgatccggca ctaaactgaa tctgcgcctt cgtacccagc
     6721 actttaccgg cttttggtga ccacggaact acgccgccat ttatccgctg cgcgctcagt
     6781 ttccattcgc tattcgggct attaaacgcc atatcacgca gttgcagacg atcggctttg
     6841 aacggtagcg gcgcggtctg gtcggtgaga ttcagcgtcc cgttttccag caggatggta
     6901 tcgacatggc gtggttcggt cagttgccga ctgcttagcg caatgtcgac actttttgcc
     6961 accagggtcg cgggctggcc atcacggcca aacgtgacgt tctccagcac gatatgagat
     7021 ggcgcggaaa aacggtgatc catcgccccg aaggccagat gatagtcgct attctcggaa
     7081 acccatgcgc tgatatgttc tgctccccag cgggtttgca gaagaaaata gaggccagcg
     7141 atcaccacta acagagcgat gagaatgtag agaagcagct tcccaataaa tttcatggtc
     7201 ttccatcccg tgaaatgcac ataagggagt tatgcacgat ttacgcgcaa tcctcaaggc
     7261 gggaatggtg aaagaggtga gactgcggcg gtaattatct taccgccgcc agtgaattac
     7321 tgtttttctg gtgggaaaat cagattcagc acgatggcag taataccgcc cgcggcgatc
     7381 ccggaggaga gcaggttttt cagccattca ggggcaaact gcaaaatcag cggctgctga
     7441 gacacgccca gaccaaccgc cagcgacagc gcgataatca gaatcgcccg acggttcagc
     7501 ggttcacgag aaacgatacg cacaccggag gcggcgatag tgccaaacat tacaagcgtt
     7561 gcgccgccca gaaccggttc tggaatgtgt tgtacaaaac cgctcactgc cgggaacaga
     7621 cccagcacga tcaacatcag cgcgacgaca aaaccgacat agcggctggc aacaccagtc
     7681 aactggatca ctccgttgtt ttgcccgaag caggagttcg ggaaggtgtt aaacaccgcc
     7741 gaaacaaacg agttcaggcc gtttgccagc acgccgcctt tcaggcgttt catgtacagc
     7801 ggaccggaca ctggctgttc ggaaacgtca gaggtcgccg tgatatcgcc aatggtttcc
     7861 agcgaagtga tcataaagac cagcatcagc ggcagcagca gactccattc aatgccaaga
     7921 ccgtaataga gcggcgttgg caccataatc agttcttgcg tcatcggttc gttgctttct
     7981 ggcaacatgc ccataaacca cgccagcgca tatccggccg ccatcgcaat caccagtgag
     8041 gccacgcgta agtaagggtt acgttgacgg ttaagcagga taattaaggc taagaccacg
     8101 cctgccagca gcagattttt cggtgcgccg aaggtgttat cgctcatggc tgcgtaaccg
     8161 ccgccaatgg acgttaaccc aacctgaatt agcgacaggc cgataatcat caccacaacg
     8221 ccagaaacca gcggcgtaat aatgcggcgc gccagatgca gaacgcggga gatcaccatc
     8281 tcggtgcaac ttgccagcat caacgtgccg aacaaagccg ccatcatggt aggaacatca
     8341 gcaccaccgg ttttcagcgc ggtaccgccc ataatcagcg gggcaacaaa gttgaagctg
     8401 gtgccctgaa tagacaacag cccggagcca accggacccc aggccttaat ttgaataatc
     8461 gatgccacac cggaggcaaa cagcgacata ctaataatgt gttgcgtgtc ttgtgccggt
     8521 aaacccagcg cctggcagat taatagcgct ggcgtgatca ccgcaacgaa catcgccagc
     8581 agatgctgac aggcggcaaa cagggtttga ggaagcggcg gacgatcttc aagacggtaa
     8641 atcagttcgc tgttttgagt ctgcgcaacc ggttgcgcat tttctgactc gagggtggaa
     8701 acagacatcg aaaacaatcc cgtggtagca aagcgggcat tttagctgac cgaaagagaa
     8761 aagcaaacgt ttgccacatc ctcatctaaa tatttaaatg agataaatgc acttttttat
     8821 ataacttttg gtatttttct gcttaaaatc catgccattt gttcattaac agctgagaat
     8881 ttgacccagg aaagcataac tcgatacccg tcatagttca tgaagttgcc actgcgcgat
     8941 gaagtatgac gagtatgaaa gagtgatgcg gatacaaagg agtaactatg tttcatctcg
     9001 atactttagc aacgcttgtt gccgcaacgc tgacgttgct gctcgggcgt aagttggtcc
     9061 attccgtctc ctttttgaag aaatacacca taccggaacc tgttgcgggt ggtttgttgg
     9121 tggcgctggc gctactagta ctgaaaaaaa gcatgggctg ggaagtcaac tttgatatgt
     9181 ccctgcgcga tccgttaatg ctggctttct tcgccaccat tggcctgaac gccaacattg
     9241 ccagtttgcg tgccggtggg cgtgtggttg gcatcttctt gattgtggtt gttggtctgt
     9301 tggtgatgca aaatgccatt ggcattggta tggctagcct gttagggctt gatccgctga
     9361 tggggctgtt ggccggttct attacgcttt ccggcggtca cggtacgggc gctgcgtgga
     9421 gtaaattgtt cattgaacgt tatggcttca ccaatgcgac ggaagtggcg atggcctgtg
     9481 caacgttcgg tctggtgctg ggcggcttga ttggcggtcc ggtggcgcgc tatctggtga
     9541 aacactccac cacgccgaac ggtattccgg atgaccagga agtcccgacg gcgtttgaaa
     9601 agccggatgt gggacgcatg atcacctcgc tggtgctgat tgaaactatc gcgctgattg
     9661 ctatctgcct gacggtgggg aaaattgttg cgcaactttt ggctggcact gcttttgaac
     9721 tgccgacctt cgtctgtgta ctgtttgttg gcgtgattct gagcaacggt ctgtcaatga
     9781 tgggctttta ccgcgtcttt gagcgtgcgg tatccgtgct gggtaacgta agcctgtcgt
     9841 tgttcctggc gatggcgctg atggggctga aactgtggga gctggcttcg ctggcgctgc
     9901 cgatgctggc gattctggtg gtacagacca tcttcatggc gttgtatgcc atcttcgtta
     9961 cctggcgcat gatgggcaaa aactacgatg cggcagtgct ggctgcgggt cactgtggtt
    10021 ttggcctcgg tgcaacgcca acggcaatcg ccaacatgca ggcgatcact gaacgctttg
    10081 gcccgtcgca catggcgttt ttggtggtgc cgatggtcgg tgcgttcttt atcgatatcg
    10141 tcaatgcgct ggtgattaag ctgtatttaa tgttgccgat ttttgccggt taaaaatgaa
    10201 aaagcgtcgg aaggaaccgg aaacgctacg tgaacactgt cgccatattt ttggtgatga
    10261 gccgccagtg ctctgtgtat gggaaacgga atttgattac gcagatgccg aactcaaggc
    10321 gctggcggct aaagagtggc agcagatcag cgaatgggat ttgagtgctt actatgtact
    10381 caatctcgtt tataacgaac cgatgcaaat agagttgttt cgctatctgt ttccgctctg
    10441 cctggcgcaa tggcatgaaa ccgttttagc tggcggttat ggtgatcact tcgaagaatc
    10501 cttaatgaag gcattgtgtc ggccctatct ctggcaggag atgatgaatg cttcccaacg
    10561 ccagcaggta cggcaatttt tactggacac cgcgttgcag cgtatggata acgagcgtgg
    10621 tttcaataac gttctttgct ggctggcggt gttcaatact ctgggtgggg ccgcgccgtt
    10681 aattcgttct ctctggtcgc gctggtgggc gctggatacg ccaggaaaag cggtgtgtgc
    10741 tattcagtat gccgcgcatc taatttatcc catagaagcc aatccgttat ggtcgcaaga
    10801 atggataggc tgggggcatc cattaggcca taaagatggc tggtcctctg ataatcgcgc
    10861 ttttcttcgc caaatgttaa cacctgaaat gattgtggct ggcgtacagg cagcggctga
    10921 aattctgcgt ggtgaaccgg aaggcgcaat ggcggcgcga attgcacagg atgcgtatga
    10981 ggcaatggat atcctcacta tccagattga agatttactg cgtgacttgt cttgtgatga
    11041 gtctggtcat gcgttggagt aacgttccgt ctccggcatc cagcgttcta tcagggcttt
    11101 tgcttgttgt gggtaacgtt cgtgaatatg tcgcgccagg cgctgaactt ccgggatcat
    11161 cgcctgatcg cgcagtaaat ccgccacttt aaactcagca ttacccgtct gacgcgtacc
    11221 taacagttca cccggtccgc gaatctctaa gtctttctgt gcaatcacaa aaccgtcgtt
    11281 gctgtcgcgc agcacttgca gccgaatttg cgcggttttg gagagcggcg ttttgtagag
    11341 cagcacgcag tgagaagcca ccgcaccacg acctacacgc ccgcgaagct ggtgtaactg
    11401 cgccagaccc agacgctccg ggttttcgat aatcatcaga ctggcgttag gcacatcaac
    11461 gccgacttca ataacggttg tggcaaccag caggtgtagc tcaccttgtt taaacgacgc
    11521 catcaccgcc tgtttctcgg caggtttcat ccgcccgtgt accaggccaa cgttcaactc
    11581 tggtagcgcc agtttcaact cttcccaggt agcttccgcc gcctgcgctt ccagcaattc
    11641 cgactcttca atcaacgtac aaacccagta tgcctgacga ccttcagtta tgcaggcgtg
    11701 gtgcacgcgg tcaatgatgt cggtacggcg ggtatcagga atagcgaccg tagtcactgg
    11761 cgtgcggcct ggcggcagct catctatcac cgaggtatcg agatcggcat acgcagtcat
    11821 tgccagcgtg cgggggatcg gcgtggcggt catgatcaac tgatgcggat ggaagccctg
    11881 ctgctggcct ttctcccaca atgccagacg ctgatgcacg ccaaaacgat gctgttcgtc
    11941 gataatcacc agcgccaggc cgttaaactg cacctgttcc tggaagatgg cgtgtgtacc
    12001 gacaatcatc tgcacctgac cgctggcgat ggcttcctgc tgtgccagcc gtgctttacc
    12061 tttctgctta ccggcgagcc agcccacttc gataccgagc ggtgcaaacc agttgcggaa
    12121 gttattggcg tgctgctcgg cgagtaattc ggttggtgcc atcaatgcta cctgtttgcc
    12181 gtgggcaatc gcacgcaacg cggcgagggc ggcgaccagc gttttaccgg aacctacatc
    12241 gccctgcacc agacgcatca tcggcacatc cagcgccata tcgcgctcga tctccgccac
    12301 tacgcgtgcc tgtgcgcccg ttggcttgaa cggtaaggcg gcgaggagtt tatttttcag
    12361 cgtgtcattg gcgctcagcg gctgggcatg aaaacgctgt gctccggcac gtaaggctaa
    12421 catgctgagg ttgtgcgcca gcagttcttc cagaatcaga cgacgttgcg ccggatgctg
    12481 cccggtttcc agatcgctaa gctgtagcgt cggtggcggg cggtgcaaag tgcgcaacgc
    12541 ttccggtagc gtcatcattc cttgtgacag ttccggcggc aggagttctt caatggcgca
    12601 ggtgtcgagc agatccagcg cctggtcggt taatttacgc agcgtggcct gctttacgcc
    12661 ttccgttgtt ggataaaccg gcgtgagcgt ttcctgtaat tctggcgtgc tgagatcgcc
    12721 ctgcacgcgg tattccgggt ggatcatctc cgcaccatat ttaccgcgct ttgcttcgcc
    12781 ataagccagt acacggcggc ccgccgccag gctatttttc attgccgcgc tgaaattgaa
    12841 aaagcgcatg gtgaggatgc cggaaccgtc gctgatctgg caggtcatca tccgccgacc
    12901 gccgaaggag atattgcagt tcagcacttc gccttccacc gtggcataaa cgcccggcag
    12961 tagttctccg atggggtaga gatgggtgcg atcttcgtag cgcaggggaa ggtgtaagag
    13021 tagatcctgc acggtatgca ggttgatttt cgccagtttg ttactaagtg ctgcgccaac
    13081 gcccgttagg gaactgagtg ggacagcatc taacaggcga cctttcatgg cacttaccct
    13141 gcagcctgca tagtagccca ccagtcggca tcagcttcga tctcgccttg ctgattgacg
    13201 tggggataag gcaggccttt gcgttttgcg actttcgcca gcaccggata gccgccttca
    13261 aacaacaggc gttgttgctc tgcttccggc aacatgctgt tttcacgcag gtacatgcct
    13321 gcattttgcc gctgacgctg ggcttcgtaa agaatgaggg ctgaggcaac ggacacattc
    13381 agcgactgca ccatgccgat catcggaatg atgatgtcct gatccgccag ggccaatgct
    13441 tcctgcgtga tgcccgtttt ctcctgtccc atcaaaatgc aggtcgggcg agtgtaatca
    13501 atttcgcgga aatcgacagc gttatcagaa agatgggttg ccagaatctg catgccctgg
    13561 cctttgagat gagcgacggc atcgccaatg gtgcggtgtg ttttcacctg tacccagctg
    13621 ttactacccg ccgctgccga agccatggtg cgcatgcggc taccaggcca gacggcgtga
    13681 acttcatgta cgccaacggc atctgcggta cgaataatcg cagaaacgtt atgaggtttg
    13741 tggacctgct ccatgcagac ggtcagatca ggctgccgcc tggcgagcat ttcgcagatg
    13801 cgtgcataac gtgttgggtt cataaaacat taatttcggt ttcgggtgac tttaatcacg
    13861 tctggcatca cgcggatttt gcgcatgata ttcgccagat gcacacggtc acgagcggtc
    13921 agacgaataa aggcgctgta gacgcgacca tctttctctt ccgtattcaa actttgaata
    13981 ttcgaggtcg tggtgttaat tgccgccgtc aggtttgcca gcgcaccctg atgattgaac
    14041 atctccacct tgatttcggt gatgaactcc tgcgccgtct ctttatccca ttccacagcc
    14101 ataaacttct ctggctcttt ctggtagcca cggatgttac ggcaggattc atggtggatc
    14161 accagacctt taccggggct gacgtgggcg ataatcgggt cgccaggaat ggggcggcag
    14221 catttcgcaa aggtgatcag cacgccatca gcgcctttga tcggcagatg cccgtggctt
    14281 tgagtcgccg gtggaatgga ggcgtcccca tgttgtagat ttttcgcgac caccacgctc
    14341 attgcgttac caaggccgat ttctgccagc agatcgtcaa gcgttgccag cttcatgcga
    14401 tccagctcgc gctgaatatt ttcctgcggg atttcattga gcttacggct accacccaaa
    14461 gcatggttga gcagacgacg gcccaggctt acagaatcat cacgcttgag gtttttcagc
    14521 aactgacgaa ttttggcgcg cgctttcgag ctaacgacaa agttcagcca agcggcattc
    14581 gggcgagcgc ccggagcggt aatgatttca acggtctgac cgctagtaag cggctgcgac
    14641 agcgggtaag gctggcggtc gacgcgtgcg cccacgcagg catggccgat atcggtatgc
    14701 actgcataag cgaagtcgac gggcgttgca ccggcaggca gctcgacaat gcgcccttcc
    14761 ggtgtgaaaa cgtaaatctc atccgggaag agatcggatt taacgctctc gataaattca
    14821 aacgaactac cggcgctctg ttgcagctcc agcaggcttt gcatccagcg ctgggcgcgg
    14881 atttgcgcgg tggtgctggt ttcgccgtgc tctttataag cccagtgcgc ggcaacaccc
    14941 atctccgcca tctgatccat atcttcggta cggatctgga cctcaaccgg cacaccgtgc
    15001 gggccgatca tcgaggtgtg caaagactga tagccgttcg cttttggaat ggcgatgtag
    15061 tctttcacgc gacccggacg cggcttgtac aggctatgca tttgcccgag cacgcgataa
    15121 caggtgtcgg aatcattgac gatcacgcgg aaagcgtaga tgtccatgat tgagtgaaaa
    15181 cgctgctctt tgagcaccat tttgcagtaa atcgaataaa gatgcttctc gcgaccactg
    15241 acgcggcacg gaattcccgc ttcctgcaaa cgcccttcga tttcagagag gattttttgg
    15301 atcatctctt tacggttgcc gcgcgcggct ttcaccactt ctttaattac gcggtaacga
    15361 ttgggataca gcgcctcaaa acccagctct tcgagttcgg ttttaatgtg gtggatacct
    15421 aaacggtgcg ccagcgggct gtaaatttcg agagtttcac gggcgatgcg gcgacgtttg
    15481 tccgggcgaa gtgagcccag cgtgcgcatg ttgtgggtac ggtcggcaag tttgatgagg
    15541 atgacgcgga tatcctgcac cattgccata atcatcttgc gaaagttttc ggcctgcgcc
    15601 tctttcttat cacggaactt gagtttatca agtttcgaca ccccctctac cagctcggcg
    15661 acgcttttac caaaaagctg ttccatatcc tggtaggtgg cgggagtatc ttcaatcacg
    15721 tcatgcagca gcgccgccat cagcgtttca tagtcgagtt tcatctcggc cagaatgcag
    15781 gcaaccgcta ccgggtgcgt gatatagggt tcaccgcttg aacgtgtttg cccctcgtga
    15841 gcatcacgtg caacgagata cgcctgccgc agacgcttga tttggtcttc cggcaggtag
    15901 gtttgaatca gttgattcag gctttcaaac agatacaagg gcgacccgct ttgtgattaa
    15961 cgacgacctt cagcaatagc ggtaacggct tgtaattcag cggcttcctg ctcttgctgt
    16021 tcctggcgtt cgcgaacgtc gaggatctgg ttgttgatca gaccttcttc gatttcgcgc
    16081 agcgcgatta cagtggtttt atcgttttct tccggtacca gcggatcctt tccgcctacc
    16141 tgcatctgac gagcgcgacg cgcggcgacc agtaccaggt caaaacggtt accaattttc
    16201 tctacagcgt cctgaacagt tacgcgtgcc atacttaaaa agctccacag gtgaagaaat
    16261 gactgggcat gatactgaaa tcaggttcag tctgccaaca atttgctgat taaagcgtca
    16321 tgacgctgct tttggcggct catgcgcaga cgttcggcgc gaataatggt cttcaaatcg
    16381 gtcaacgcgg tatcgaagtc atcattcaca atcagataat catattcggc gtaatggctc
    16441 atttctgcaa cagcttgcgc catacgcttt gcaatgacct cttcgctgtc ctgaccgcga
    16501 ccgcgtagac ggcggtccag ttcaattttg gacggcggta aaataaagat actccgcgcg
    16561 tgcggcatct tctggcgaat ttgctgcgcg ccctgccagt cgatatcgag aaaaacatcg
    16621 acaccggtcg ccagtacttg ctcaatggcc tcacgcgaag tgccatagta attaccaaaa
    16681 acttctgcgt gttcgaggaa cgcatctctg ctaatcattt ctttaaattc atcatgatta
    16741 acaaagaaat aatgttcacc gtggacttca ccaggacgcg gttggcgtgt ggtgtgtgaa
    16801 acagaaacct gggtgtcata caacggttgg gtttttaata aagcctgaat caggctggat
    16861 ttacccgcgc cactgggggc agaaacaata taaagcgtgc cttgagccat gagtatcttt
    16921 cgtatgtgat tagcgaaata aagcctacat acgagcttat tatacacggc gcggccatgt
    16981 gacgtagccc ttgctcacac tttttgcgca aagttgttgc gttttgcgta gctgtttccg
    17041 catttgcctg tgatttactg caataaagac gattctcagg aagcgtcctc gcagactccg
    17101 tttttgccac tcggctttgc cttcaccacg ttgtatatct gccagcctga ctacagcgga
    17161 ggtgaagatg aaagtatgga tggcgatatt aataagtatc ttgtgctggc aatcatctgt
    17221 gtgggcggtc tgtccggcct ggtcgccagc cagagcacag gaagaaattt cccgcctgca
    17281 acagcaaata aaacagtggg acgatgacta ctggaaggaa ggaaaaagtg aggtggaaga
    17341 cggtgtttac gatcagttaa gcgcccgtct tacgcagtgg caacgctgct ttgtgagcga
    17401 gccccgcgac gtgatgatgc cgcctttaaa tggtgcggtt atgcatccgg ttgcccatac
    17461 gggtgtacgc aaaatggcgg ataaaaacgc attaagtctg tggatgcgag aacgtagcga
    17521 tctttgggtg cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc gggacgggaa
    17581 actgaacaaa gcaatcagtc gcggtaatgg cctgaaaggc gaggactgga cgcagaaagt
    17641 tagcttaatt tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca gtacgcttca
    17701 gggggaaata tttctccagc gcgaggggca tatccaacaa caaatggggg gaataaatgc
    17761 ccgcgcaaaa gttgctggcc tgatgatgcg ccaggacgat agcgacacgc tgaattctct
    17821 gggcgttttt gtctgggcat ggccggatgg accgcagtta atgaccgatc gtttaaagga
    17881 gctggctacc gcaggtttta ctctaacgca gaggtatacc cgtgcggtta aaaatgctga
    17941 tgaggttgcg cgcgtacgca atgagtggtg gaaagcgaaa ttacccttcg tcaccgatgg
    18001 cgtggttgtg cgaggggcga aagaacccga atcccgccat tggctaccag gccaggcaga
    18061 atggctggtg gcctggaaat atcaacctgt agctcaggtt gccgaagtga aggcgattca
    18121 gtttgcggtg ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac ctgtcatgct
    18181 ggatgataaa aaagtccagc gggtgaatat tggttccgtc aggcgctggc aggagtggga
    18241 tattgcgcct ggtgatcaga tcctcgtcag ccttgccggt cagggtattc ctcgcattga
    18301 tgatgtggtc tggcgcggtg cagaacgtac aaaaccgaca ccaccagaaa accgctttaa
    18361 ctcgttgacc tgctactttg cttctgatgt ttgtcaggaa cagttcattt cacgcttagt
    18421 ctggctgggg tcaaaacagg ttctggggct ggatggtatt ggtgaggccg gctggcgcgc
    18481 gctgcatcag actcatcgct ttgagcatat cttttcctgg cttttattaa cgccagagca
    18541 attacagaac acgccgggga tcgcgaaaag taaaagtgcg cagctatggc atcggtttaa
    18601 tctggctcgt aagcagcctt ttactcgctg ggtgatggca atgggaatac cgctaacccg
    18661 ggcggcgctt aatgccagtg atgaacggtc ctggtcgcaa cttttattta gcacggagca
    18721 gttctggcag cagctgccgg ggactggatc ggggcgcgcc agacaggtta ttgaatggaa
    18781 ggaaaatgcg caaatcaaga agctgggcag ttggctggct gcccagcaga tcacaggttt
    18841 tgaaccttag tgtccttcgt ggctgtagta aaagaccggt aatcccagct taaggcgtag
    18901 cgccagtaag cgggcaaaaa agccgaatac cagggtggag ataattacca catcatggtt
    18961 agaaacatag tgttgcagcg cgatgtacag cacggcggag gcaaatgaga caccggcata
    19021 caactctttc tggaatacca gtgggatacg tttacagaac atatcgcgca aaacgccgcc
    19081 aaacacgccg gtggttaccg ccgcgacaac ggcaataatt gggccgtgcc ccatatccag
    19141 tgcaacctgt gcgccgatga tagaaaagac caccagtcca agcgcatcga gcaccagaaa
    19201 cactttgcgc aggtaaggca ttacaggagc aacgatagtg gtgagtactg cggcggtagc
    19261 gacgataatc acatattccg ggtgtttgac ccagccgagc ggatagtggc ccagcagaat
    19321 atcgcgcact gaccctccgc caattgcggt ggcggtcgca ataataatta cgccaaatgt
    19381 atccatgcgg cgtcgcccgg ccgccagagc ccccgtcatg gcttcggcag taatgccaac
    19441 caaatacaaa atgtgtaaca gcatggttct ctccctaaaa atctgtctgg cagggtagcg
    19501 atttgtgcgt attgtcacga ttgagatttt ttaaggtgag aggaaattga taacttgtag
    19561 ttatgtgaaa tcgctatttt ctgtagcgta gatgcattct tctgacttct tgtctgagaa
    19621 aatgcataag aaaaaatagg ttaaaattga ggggtggaaa agaaaggtgc aatgtttagc
    19681 cattgtgttc accgcacctg gactttggct tacgcatcgg gtatctcaat caacaaaaag
    19741 agtttttagt ttctgctatt tcttctctgt aatttttaca ctgttttcca actgcttgat
    19801 gctgacctgc ggggtcggca actcctcagg catggttccg ccaagttcct gaatggtttg
    19861 cctcaccttg ctacccacgt caaagtgtgt ggtattagct tgctgttttg aattcacgcc
    19921 atcccgcttg agtttttctt ctgtttgggt agctcgaaag agattagccg ccagttctgt
    19981 tgaacccata tgatccagga tcttctgact ctttttcagc cccttccgct gatggatggc
    20041 tttctgatct aatccaccat acagcccccg gtaaccatga ttctgaaata tggcgaaatc
    20101 gatagctgtc gctaccccag cttgctgcgc tgcctcaacc aattgtttgt tatgttcttt
    20161 caattcattt ctcagaaaaa gacgcttttc gtcttcacga agttgcctga aagcttcatc
    20221 gtcagccagc tcctgccgtc gggtctggat agcaaaataa gtttgccctg ccgcaataac
    20281 cggtttcgca gggtcgccgt tttgtactac caggtaacag gcatagcggg agagatgaac
    20341 atcttcaagt tctcgttgag cccctgaacc taatacaacc attttggtgg tttcaacgaa
    20401 atggtcagat gcagcttgat tgctggcttc gcacgcttga gtcgcgcggg ccagtacctt
    20461 ttgaaaattg cgccagtccc gataatccag taacggtgcc aattctctag ctgaccaaaa
    20521 ttcttgccct tcagtgccat aatgccgtat ttcttcaaaa ggttgatgat gttcgttcat
    20581 tgcccactcc atgtgatatc gattgcaaag aaagtaaatc tgtaactgta tttatataca
    20641 gttgtgagta tctcacgttc tcagtgtgct gggcaaaaaa acatcgcaaa atctggagca
    20701 ggcacgcaaa acagaattga agttgcgggg aagaaaaatg ccgctctgtt tgagtgggcg
    20761 gcatttgtga aggctgaaaa gtaagaatta gtgttacgag ttactcaata ttctgaatct
    20821 gctcgcgcat ctgctcaatc aacactttca gctcaatcgc ggagtttgtc acttcggcat
    20881 tgatagactt cgacgcaaga gtgttcgact cgcggttgaa ttcctgcatc ataaagtcca
    20941 gacgacgacc aacggcttct tttttcttca gaatgttgta ggtctctttg acatgcgctt
    21001 ccaggcggtc cagttcttcg gcaacgtcaa ttcgttgtgc cagcagaacc aactcctgct
    21061 ccaggcggtt gttttccagt tgcacttgag catcttccag cttcgcgacc agacgctcac
    21121 gctgccattg caggatttcc ggcatatggg cgcggacttt gaccacttcg gcggtgacgc
    21181 cttccagacg ctgctcgatc aatgctttca gcgcctgacc ttcggtttca cgtgcgacga
    21241 taaagtcgtc cagggtacca tccaacgccg cgagaatttc agcggcaatc gcgtcgagat
    21301 cctgctcctg ggctgccatt acgcccggcc agcgcagaat atcaaccggg ttgatttccc
    21361 cttcgtcact ctgcattttt acccagttcg cggcagttac cagctgttta gccagttttt
    21421 cgttgaggat cagctcacct tgcgcgctaa catctggctc atagcgcagg gtacattcca
    21481 ctttaccgcg cgtcaggcga gaacgaatac gctcgcgaac gacaggttca aggctacgga
    21541 actgctccgg cagacgaaag taagtttcca gataacgctg gtttaccgag cgcatttccc
    21601 aggttgcgct cccccattca cccttgattt cacgccgggc gtaggcggtc atactgcgga
    21661 tcatagacgt tcctgtttat aaaaggagag gtggaaggat tatagccatc gatgccttgt
    21721 caggatagga ataaccgccg gaagtccgta taatgcgcag ccacatttgt ttcaagccgg
    21781 agatttcaat atgcgtccag caggccgtag caataatcag gtgcgtcccg ttaccctgac
    21841 tcgtaactat acaaaacatg cagaaggctc ggtgctggtc gaatttggcg ataccaaagt
    21901 gttgtgtacc gcctctattg aagaaggcgt gccgcgcttc ctgaaaggtc agggccaggg
    21961 ctggatcacc gcagagtacg gcatgctgcc acgttctacc cacacccgta acgctcgtga
    22021 agcggcgaaa ggtaagcagg gtggacgcac aatggaaatc cagcgtctga tcgcccgtgc
    22081 tcttcgcgcg gcagtagatt tgaaagcgct gggtgagttc accattacgc tggactgcga
    22141 cgtgcttcag gctgatggtg gcacgcgtac cgcgtcgatt acgggtgcct gcgtggcgct
    22201 ggcagatgcg ctacagaagc tggtggaaaa cggcaagctg aaaaccaacc cgatgaaagg
    22261 gatggtagcc gcagtttctg tcggtattgt aaacggtgaa gcaatttgcg atctggaata
    22321 cgttgaagac tcagccgcag agaccgacat gaacgtagtg atgaccgaag acgggcgcat
    22381 cattgaagtg caggggacgg cagaaggcga gccgttcacc catgaagagc tactcacctt
    22441 gttggcgctg gcccgagggg gaatcgaatc cattgtagcg acgcagaagg cggcgctggc
    22501 aaactgattt ttaaggcgac tgatgagtcg cctttttttt gtctgtagaa aagtaagatg
    22561 aggagcgaag gcatgaaacc atatcagcgc cagtttattg aatttgcgct tggcaagcag
    22621 gtgttaaagt ttggcgagtt tacgttgaag tccgggcgca aaagccccta tttcttcaac
    22681 gccgggctgt ttaataccgg gcgcgatctg gcactgttag gccgttttta cgctgaagcg
    22741 ttggtggatt ccggccttga gttcgatctg ctgtttggcc ctgcttacaa agggatcccg
    22801 attgccacca ctaccgccgt ggcgctggcg gagcatcatg accttgacct gccgtactgc
    22861 tttaaccgca aagaggcaaa agaccacggt gaaggcggca atctggttgg tagcgcgtta
    22921 caaggacgcg taatgctggt agatgatgtg atcaccgccg gaacggcgat tcgcgaatcg
    22981 atggagatta ttcaggctaa tggcgcgacg cttgctggcg tgttgatttc gcttgatcgt
    23041 caggaacggg ggcgcggcga gatttcggcg attcaggaag ttgagcgtga ttacaactgc
    23101 aaagtgatct ctatcatcac cctgaaagac ctgattgctt acctggaaga gaagccggaa
    23161 atggcggaac atctggcggc ggttaaggcc tatcgcgaag agtttggcgt ttaaagaaac
    23221 tcgccggatg aaaagtcatc cggcgtcata ttactgcaac tgtgccgcaa ttagcggcca
    23281 gcgggcgtca aaatcatccg tcgggcggta tttaaattcg ctgcggacaa aacgtgacag
    23341 cataccttca cagaaggcca ggatctggct tgccagcagg gtttcatcgg tggtgtaacc
    23401 ttcaccctca cgcattctct tttcacgcaa tacctggcgc agctgcgctt caatacgctc
    23461 gaacagctgg ttgatgcgcc cttgcaggcg atcctgttca aacattagcg catgaccagt
    23521 gaggatgcgg gtcaggccag gattacgctc accaaaaccg agaagcagca acacaatcag
    23581 acgcaggcgc gctgtggtgt ctttctcatc tttcagaatc aggttgatgc gagtaatcag
    23641 gctatcttcg ataaactcaa tcaggctatc gaacatgcgg gtcttactgg ggaagtggcg
    23701 atacagtgcc gcttcggaaa cgccgacaga ggcggccagt tttgccgtcg tgatacgttg
    23761 gcttccatcg ctggattcca gcatcagcgc cagagactga agtatttcct cgcgacggtt
    23821 ccttttcgca gtttgttttt ctgccatgtt acaaaatacc cctgaaaata agcacttgcc
    23881 aggcggcacc cacgctatga ccgcaaacga aatgtttgcg gctatgttat gacgttattc
    23941 ggatgcgtat gtgttactga cgaccagagt gaccaaagcc gccttcaccg cggtcggtgg
    24001 cgtcgaaatc ttccaccaga ttaaattcag cctgtactac cggaacaaaa atcatctggg
    24061 cgatgcgttc gccaggttga atggtgaagc tgtcctgacc acggttccac acggaaatca
    24121 tcaactggcc ctgatagtca gaatcgatca atcctaccag gttaccaagc acgataccgt
    24181 gcttatgtcc caatccggag cgcggcagca tcattgccgc cagtgaagga tcggcaatat
    24241 gaatcgccag cccggtcgga accagcgtag tgtcacccgg agccagttct acggcgtcgt
    24301 cgagacaggc acgcaggtca agtccggcag agccagaggt ggcataagtc gggagcggaa
    24361 attccttccc aacgcgcggg tccagaatct taacgtcgat ttttttcatc ataacgggtc
    24421 acgatctcgt cgagtaataa ttggccaagg agctctttgc gctcaagcgg taagacttta
    24481 tctccgtcct gccagaaaag gtgtaatgcg ttgttgtcgc tgttaaatcc ttgagttggc
    24541 tgggaaacat cgttcgcgca gatcagatca aggtttttac ggatacgttt ttgccgggcg
    24601 tattcttcca cattatttgt ttcggcggca aatccaacga cgtagggtcg atggtctttt
    24661 agtgcggcaa cgcctgcgac gatatcgggg tttttaacca tttttattgt taattcatca
    24721 ccctgcgtgg cctgcttttt gattttctct ggggccacgg tagctgcgcg ataatccgcc
    24781 acggcggcgc agccgataaa aatattttgc tgctgtacag aagcattcac ggcggcttcc
    24841 atttccagcg cagtcatcac atcaacacgt ttaacaaacg gtggcgtcgg tagtgaaacc
    24901 ggacctgata ccagcgtgac gttcgcgcca cgacgggcag cggcggcggc gatagcaaaa
    24961 cccatcttgc cggagctgtg attagagata taacgcaccg gatcgagcgg ttcacgcgtc
    25021 ggaccggcgg taatcataat gttcagatgt ttcaggtcgt tgacgggcga aaaatgcgcc
    25081 accgccatat ccacaatggt taacggatcg agcattcgcc caggaccgat atcaccacaa
    25141 gcctgactgc cactgtctgg cccccagatg agcaaaccac gggaagcaag cacctctaaa
    25201 ttatgctgcg tggcagcggc acggtacatc tgctggttca tggcggggag cacggctaca
    25261 ggcgcaggtg tagccagaca aatcgtcgat accaggtcat tcgccattcc ggcagcaaca
    25321 cgggcaatca aatctgccgt ggcaggggcg agaatcacta aatcagccca tttacccagc
    25381 tcaatatggc ccatagcggc ttcggctgcc gggtccagca aactgtcgga aacgggataa
    25441 ccagaaaccg cctgcaagct aagtggggtg ataaaggctt ttgccgcttc ggtcatggct
    25501 acgcggacgt cggccccgcg atcgcgcaaa cgacgcacca gttcaggggt tttataggca
    25561 gcaataccgc cgctaacgcc gagaacgatt tttttaccgg ccaggctcat gatgattttt
    25621 cctgttgggt gacaccagaa gttggcgatt ttatcacaat acttttgttg tcgtgccttc
    25681 acctgagaat cactttgcga ggcgctttcc aggattgaaa actggccgtc gatttaacgg
    25741 aacggctatg acaggatgcg agcaccacaa aggaggtgaa ggtgaaaaac aatgcacagc
    25801 tgttgatgcc gcgcgaaaaa atgctgaagt ttggtattag cgccttaacg gatgtcgagc
    25861 tgctggcgtt atttctgcgt accggaacgc gcggtaaaga tgtattaacc ctggcaaaag
    25921 agatgctgga gaatttcggc tctctttatg gcttgttaac ctctgaatat gaacaattta
    25981 gtggcgttca tggaattggc gtagcgaaat ttgcccagtt aaaggggatt gctgaactgg
    26041 cgcggcgtta ctacaacgtg cggatgcgtg aagaaagccc tttactcagc ccggagatga
    26101 cgcgggaatt tttacaaagc cagctcacgg gtgaggagcg ggagatcttt atggtgatct
    26161 ttctcgactc acaacaccgg gttataacgc atagccgtct tttttccggc acgctaaacc
    26221 atgttgaagt ccatcctcgg gaaattatcc gcgaagcgat aaaaataaac gcctcggcgc
    26281 tgatccttgc acataatcac ccttcgggtt gtgctgaacc cagtaaagcg gataaactca
    26341 ttactgaacg gataataaag agttgtcagt tcatggattt acgcgtgctc gaccatatcg
    26401 tgattgggcg tggagagtat gtttcttttg ccgaacgcgg ctggatttaa cccgctatgc
    26461 gcgatccttc gggatctttg tctgttcggg acttgagcac atcgctgagt cagcgtatac
    26521 tacgccacct ttgagaatct cgggtttggc atttgggcct ggcaatcgag agttcacata
    26581 gaactgcgat gaccgggctg taaagcctga cgaggcgcca ataccccata cgaagctcga
    26641 gctaatttga tttttggaga atagacatgt cccgagtctg ccaagttact ggcaagcgtc
    26701 cggtgaccgg taacaaccgt tcccacgcac tgaacgcgac taaacgccgt ttcctgccga
    26761 acctgcactc tcaccgtttc tgggttgaga gcgagaagcg ttttgtcacc ctgcgcgtat
    26821 ctgctaaagg tatgcgtgta atcgataaaa aaggcatcga tacagttctg gctgaactgc
    26881 gtgcccgtgg cgaaaagtac taagtactta gaggaaataa atcatggcta aaggtattcg
    26941 tgagaaaatc aagctggttt cttctgctgg tactggtcac ttctatacca ctacgaagaa
    27001 caaacgtact aagccggaaa aactggaact gaaaaaattc gatccagttg ttcgtcagca
    27061 cgtgatctac aaagaagcga aaatcaaata attctcgctt tgatgtaaca aaaaacctcg
    27121 ctccggcggg gttttttgtt atctgcttgc ccccatattg attgcatctg ttcattcctg
    27181 gagatgctat gcctgaatta cccgaagttg aaaccagccg tcgcggcata gaaccgcatc
    27241 tcgttggtgc aaccattctt catgcagtgg tgcgcaacgg acgcttgcgc tggccggttt
    27301 cagaagagat ctaccgttta agcgaccaac cagtgcttag cgtgcagcgg cgggctaaat
    27361 atttgctgct ggagctgcct gagggctgga ttatcattca tttggggatg tctggcagcc
    27421 tgcgcatcct tccagaagaa cttccccctg aaaagcatga ccatgtggat ttggtgatga
    27481 gcaacggtaa agtgctgcgc tacaccgatc cgcgccgctt tggtgcctgg ctgtggacca
    27541 aagagctgga agggcataat gtgctggccc atcttggacc ggagccgctc agcgacgatt
    27601 tcaatggtga gtatctgcat cagaagtacg cgaagaaaaa aacggcgatt aaaccgtggc
    27661 tgatggataa caagctggtg gtaggggtag ggaatatcta tgccagcgaa tcactgtttg
    27721 cggcggggat ccatccggat cggctggcgt catcactgtc gctggcagag tgtgaattgt
    27781 tagctcgggt gattaaagcg gtgttgctgc gttcgattga gcagggcggt acaacgctga
    27841 aagattttct gcaaagtgat ggtaaaccgg gctatttcgc acaggaattg caggtttacg
    27901 ggcgaaaagg cgagccgtgt cgggtgtgcg gtacgccgat tgtggcgact aaacatgcgc
    27961 agcgggcaac gttttattgt cggcagtgcc agaagtaatt catgcgcgcc ggatggcata
    28021 ccatccggca taaacgctac gctaacttcg ccatcagcgc ctgatggaca ttctccggca
    28081 ggaaatgggt gacatcgccc tgatggcgcg ccacctcttt caccaacgat gaagagataa
    28141 acgaccactc tttcgacggc atcagaaaca cactttccag ttccggcatt aagtggcgat
    28201 tcatatgcgc cagctgcatt tcatattcaa aatccgccac cgcacgcagg ccacgaatca
    28261 gcaccgtagc gtgttgatta cgggcgaagt tcgccatcaa atcactaaac ccgaccactt
    28321 ccacgttccc cagatgcgcg gttgcctgct gtgccagcgc cacacgctct tccagggtaa
    28381 acatcggttt tttactgggg ctggcggcaa tcgccagaat aacgtgatcg aacatttgcg
    28441 tggcgcgcgt cacgatatcg atatgaccat tggtaatggg atcgaaagta cccggataaa
    28501 tcgcccgttt ttgcataaca acctcaatgc gttttcggtg gcaggtaagg ttccagcagt
    28561 tgaagcagac gctgtagcgc gccctggttt tgatacagta cttcaacggc atgacggcca
    28621 tagaaactac ggtaatcggc gtcggtgagt aaagaggaca cctcttttgc aagcgtagtg
    28681 gcatcggtaa cggtaatcag cccgcttgcc tgctccagcc gcgcgcaaat gtctttaaag
    28741 ttaaaagtat gcggccccat caataccgga atagcgtgtg cggcagcttc cagcggatta
    28801 tgcccaccac gttcaaccag tgaaccgcca acaaaggcga gatcggcaat gccgtacagt
    28861 aacatcaact cgcccatcgt atcgccaacc acaacctgcg tgctggtgga ggggacttcc
    28921 cctgaagagc gtgtgatata gcttagtcca gcctggcgga caagattaat cgcatccggg
    28981 aagcgttctg gatgacgggg taccaggatg agcaataaat tcgggaattg ctgtaacaat
    29041 gcctgatgtg cggcgatcac cacactctct tcgccttcgt gagtgctggt ggcaatccat
    29101 accgggcggt gtggtgccca ctggcggcgc agcgtcacgg ctttagcagc caactgcggc
    29161 gttacagaaa tatcgaattt caggctaccg gtaacggtca cctgattatt ttttgcgccc
    29221 agcgccacaa aacgtgcacc atcttcttca ttttgcgcag caatcagcgt aatacgacgc
    29281 agcaagcgac ggacgaattt acccagtttg gcataacctg cggccgagcg ggcagagagt
    29341 cgcgcgttag cgatcaccag cggaatttta cgtttgtgta gcgccgcaat caggttaggc
    29401 catagttcgg tttccataat caacaccagt ttggggtcga ctttattcag gaaacggttg
    29461 agcgcatcgg gcaggtcata cggcagataa acgtgctgaa catccttccc gaaagccgat
    29521 tgtacgcgct ccgaaccggt tggcgtcatg gttgtaacgg taatcggtaa atcaggataa
    29581 cgatggcgca gcgcgcgcac caacgggatc gccgccagag tttcaccgac ggagacggag
    29641 tgcagcataa tgccgcctgg ttttagcgga tggcggtaaa aaccgtaacg ttcaccccag
    29701 cgttttcgat aggccggagc cttacgtccg cgcacccaga gccgtatcca gatcagcggc
    29761 tgaataaggt agagaagggc ggtgtaaagc aattcgagca tagtaaatag ctgacttatg
    29821 gatgtgctgg ggattctatg tatttagctg tggctttacc attacttttc ccgtttttga
    29881 cttaaatagc aatttacccg ccaattcttg ctgaacatct atactttttt tgtattttta
    29941 tgcgattcat tgatttctgc tgtacgtgca ggaaggaatc gcagagcaac agtgaaagaa
    30001 tatggttgct aatgatttta tttgattgat ttctttattg aaaatcaaca atgtgcttaa
    30061 aaatcattcg ggtcggattg ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc
    30121 ggtatcagta ttcaggtagc tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc
    30181 ttaatcggat aatctcaaca aaaagagtta cttgtggata agccatttcg aagaattttg
    30241 ctcattaaga tgcgttttca tggggatatg ttattaacta ctcccgtcat tagttcgctg
    30301 aaaaaaaatt accctgacgc aaaaatcgat gtgctgcttt atcaggacac catcccgatc
    30361 ctgtctgaaa atccagagat taacgcgctc tacggcataa aaaataaaaa agcaaaagcc
    30421 tcagaaaaaa ttgccaactt ttttcatctc atcaaggtat tacgtgccaa taagtatgac
    30481 cttatcgtca atctcaccga tcaatggatg gttgctatac tggttcgctt attaaatgcc
    30541 cgtgtgaaaa tttcccagga ttatcatcat cggcagtctg ctttttggcg taaaagtttc
    30601 acccatttgg tgccgttgca gggtggaaat gtggtggaaa gtaacttatc cgtgctgacc
    30661 ccattgggag ttgattcgtt ggtgaagcag acaaccatga gttacccgcc tgcaagctgg
    30721 aaacgtatgc gtcgcgaact tgatcacgct ggtgttggac aaaattatgt ggttatccaa
    30781 cctacggcgc ggcaaatctt caaatgctgg gacaacgcca agttttccgc tgtgattgat
    30841 gccttacatg ctcgtggtta tgaagtcgtt ctgacgtccg gcccagataa agacgatctg
    30901 gcctgcgtca atgaaattgc gcagggatgc cagacgccac cagtaacggc gctggctgga
    30961 aaggtgacct tcccggaact tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt
    31021 gattccgcac cggcgcatat tgccgctgca gttaatacgc cgctgatatc gctgtttggt
    31081 gcgacagatc atattttctg gcgtccctgg tcaaataaca tgattcaatt ctgggcggga
    31141 gattaccggg aaatgccaac gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt
    31201 attccggcgg cagatgtcat tgctgctgtc gataaattac tgccctcctc cacgacaggt
    31261 acgtcgttat gatcgttgct ttttgtttat ataaatattt tccctttggc ggtttgcagc
    31321 gtgattttat gcgtattgct cagacagtcg ccgcccgagg tcatcatgtt cgggtttata
    31381 cccagtcgtg ggaaggcgaa tgccctgatg tatttgaact gatcaaagtg ccggttaaat
    31441 cgcataccaa tcacgggcgc aatgcggagt attttgcctg ggtgcaaaaa catttacgcg
    31501 aacatcccgt cgataaagtc gttggattca acaaaatgcc ggggctggac gtttattatg
    31561 ccgctgatgt ttgttatgcc gagaaagtag cgcaggaaaa aggctttttc tatcgcctga
    31621 cgtcacgtta tcgccattat gccgcctttg agcgggcaac cttcgaacag ggtaagccga
    31681 cacagctgct gatgctgaca gataagcaaa tcgccgattt ccagaaacat tatcagactg
    31741 aagcggagcg ttttcatatt ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc
    31801 agccagccaa tagccgtgaa atcttccgta agaagaatgg aataaccgaa caacaatatt
    31861 tattgttgca ggtcggttca gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac
    31921 ttgcttcgtt accggattcg ctgcgccaca acacattgct atatgttgtt gggcaggata
    31981 aaccgcgaaa atttgaggcg ctggcagaaa aacgcggcgt gtgcagtaat gttcactttt
    32041 tctcggggcg caatgatgtc tcggaattaa tggcggcggc ggatttatta ctgcatcctg
    32101 cctaccagga agcggcggga attgtgctgc tggaagcgat tactgcagga ttaccggtac
    32161 taacaacagc cgtttgtggc tatgcgcctt atattgtcga ggctaattgc ggcgaggcta
    32221 ttgctgagcc attccgccag gaaacattga atgagatttt acgcaaagcg ttaacgcaat
    32281 cttcattgcg ccaggcctgg gcggaaaatg cgcgatatta tgctgataca caagatttat
    32341 acagtctgcc agagaaagcg gcggatatca taacgggtgg tctggatggt tgaacttaaa
    32401 gagccgtttg ccacgttatg gcgcggcaaa gatccttttg aggaagttaa aaccttgcag
    32461 ggtgaggtat ttcgtgaact ggaaactcgc cgtactctgc gctttgaaat ggcgggcaaa
    32521 agctattttc tcaaatggca tcgcggcacg accctgaaag agataatcaa aaatttactc
    32581 tcattgcgga tgccagtatt aggcgcagac cgcgaatgga atgcgattca tcgactgcgg
    32641 gatgtcggcg ttgatactat gcatggggtg gcatttggcg aaaaaggcat gaatccgctg
    32701 accagaactt catttattat taccgaagat ctgacaccaa ccataagtct ggaagattac
    32761 tgtgctgact gggcgactaa ccctccagat gttcgcgtaa agcgtatgct tattaagcgt
    32821 gtcgcgacga tggtgcgcga tatgcatgct gcgggcatta accaccgtga ctgttatatc
    32881 tgtcatttcc tgctgcactt gcctttttcc ggtaaggaag aggagttaaa aatttcggta
    32941 attgacctgc accgggcgca gcttcgcacg cgcgttccac gtcgttggcg ggataaagat
    33001 cttattgggc tttatttttc ttcgatgaat atcggcctga ctcagcggga tatctggcgg
    33061 tttatgaaag tgtattttgc cgccccgctt aaagacattc tcaagcagga acaaggactg
    33121 ctgtcgcaag cagaagcaaa agccacaaaa atcagggaaa gaacgattcg aaaatcgttg
    33181 taacccgtaa cttatttttg ccaaaatttt ggatacagaa taaatatgtc tcaactcaat
    33241 gatagtgaca tcatcctttt tgagtataat tttcattat
//
LOCUS       DAZKGS010000034        33043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKGS010000034 DAZKGS010000000
VERSION     DAZKGS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..1037
                     /gene="pspF"
                     /locus_tag="ACHOQY_001639"
     CDS             60..1037
                     /gene="pspF"
                     /locus_tag="ACHOQY_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9677765.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(1157..2422)
                     /gene="puuE"
                     /locus_tag="ACHOQY_001640"
     CDS             complement(1157..2422)
                     /gene="puuE"
                     /locus_tag="ACHOQY_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP9677766.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(2460..3740)
                     /gene="puuB"
                     /locus_tag="ACHOQY_001641"
     CDS             complement(2460..3740)
                     /gene="puuB"
                     /locus_tag="ACHOQY_001641"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP9677767.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(3742..5229)
                     /gene="puuC"
                     /locus_tag="ACHOQY_001642"
     CDS             complement(3742..5229)
                     /gene="puuC"
                     /locus_tag="ACHOQY_001642"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP9677768.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(5504..6061)
                     /gene="puuR"
                     /locus_tag="ACHOQY_001643"
     CDS             complement(5504..6061)
                     /gene="puuR"
                     /locus_tag="ACHOQY_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP9677769.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(6088..6852)
                     /gene="puuD"
                     /locus_tag="ACHOQY_001644"
     CDS             complement(6088..6852)
                     /gene="puuD"
                     /locus_tag="ACHOQY_001644"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP9677770.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            7064..8482
                     /gene="puuA"
                     /locus_tag="ACHOQY_001645"
     CDS             7064..8482
                     /gene="puuA"
                     /locus_tag="ACHOQY_001645"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP9677771.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            8631..8795
                     /locus_tag="ACHOQY_001646"
     CDS             8631..8795
                     /locus_tag="ACHOQY_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677772.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            8785..10170
                     /gene="puuP"
                     /locus_tag="ACHOQY_001647"
     CDS             8785..10170
                     /gene="puuP"
                     /locus_tag="ACHOQY_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP9677773.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            10304..10549
                     /gene="ymjA"
                     /locus_tag="ACHOQY_001648"
     CDS             10304..10549
                     /gene="ymjA"
                     /locus_tag="ACHOQY_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP9677774.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            10862..12505
                     /gene="sapA"
                     /locus_tag="ACHOQY_001649"
     CDS             10862..12505
                     /gene="sapA"
                     /locus_tag="ACHOQY_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9677775.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            12502..13467
                     /gene="sapB"
                     /locus_tag="ACHOQY_001650"
     CDS             12502..13467
                     /gene="sapB"
                     /locus_tag="ACHOQY_001650"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9677776.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            13454..14344
                     /gene="sapC"
                     /locus_tag="ACHOQY_001651"
     CDS             13454..14344
                     /gene="sapC"
                     /locus_tag="ACHOQY_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9677777.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            14344..15336
                     /gene="sapD"
                     /locus_tag="ACHOQY_001652"
     CDS             14344..15336
                     /gene="sapD"
                     /locus_tag="ACHOQY_001652"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9677778.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            15338..16144
                     /gene="sapF"
                     /locus_tag="ACHOQY_001653"
     CDS             15338..16144
                     /gene="sapF"
                     /locus_tag="ACHOQY_001653"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9677779.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            16212..16565
                     /gene="ycjD"
                     /locus_tag="ACHOQY_001654"
     CDS             16212..16565
                     /gene="ycjD"
                     /locus_tag="ACHOQY_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFP9677780.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            16933..17721
                     /gene="fabI"
                     /locus_tag="ACHOQY_001655"
     CDS             16933..17721
                     /gene="fabI"
                     /locus_tag="ACHOQY_001655"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9677781.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            17866..18995
                     /gene="yciW"
                     /locus_tag="ACHOQY_001656"
                     /pseudo
     CDS             17866..18995
                     /gene="yciW"
                     /locus_tag="ACHOQY_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            19063..20997
                     /gene="rnb"
                     /locus_tag="ACHOQY_001657"
     CDS             19063..20997
                     /gene="rnb"
                     /locus_tag="ACHOQY_001657"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9677782.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(21075..21178)
                     /locus_tag="ACHOQY_001658"
                     /pseudo
     CDS             complement(21075..21178)
                     /locus_tag="ACHOQY_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21232..23217
                     /gene="pdeR"
                     /locus_tag="ACHOQY_001659"
     CDS             21232..23217
                     /gene="pdeR"
                     /locus_tag="ACHOQY_001659"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9677783.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(23199..23327)
                     /gene="ymiD"
                     /locus_tag="ACHOQY_001660"
     CDS             complement(23199..23327)
                     /gene="ymiD"
                     /locus_tag="ACHOQY_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9677784.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            23365..23538
                     /gene="yciZ"
                     /locus_tag="ACHOQY_001661"
     CDS             23365..23538
                     /gene="yciZ"
                     /locus_tag="ACHOQY_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9677785.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            23628..24377
                     /gene="yciT"
                     /locus_tag="ACHOQY_001662"
     CDS             23628..24377
                     /gene="yciT"
                     /locus_tag="ACHOQY_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9677786.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            24646..24864
                     /gene="osmB"
                     /locus_tag="ACHOQY_001663"
     CDS             24646..24864
                     /gene="osmB"
                     /locus_tag="ACHOQY_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9677787.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(24990..25316)
                     /gene="yciH"
                     /locus_tag="ACHOQY_001664"
     CDS             complement(24990..25316)
                     /gene="yciH"
                     /locus_tag="ACHOQY_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9677788.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(25316..26053)
                     /gene="pyrF"
                     /locus_tag="ACHOQY_001665"
     CDS             complement(25316..26053)
                     /gene="pyrF"
                     /locus_tag="ACHOQY_001665"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9677789.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(26245..27414)
                     /gene="lapB"
                     /locus_tag="ACHOQY_001666"
     CDS             complement(26245..27414)
                     /gene="lapB"
                     /locus_tag="ACHOQY_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9677790.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(27421..27729)
                     /gene="lapA"
                     /locus_tag="ACHOQY_001667"
     CDS             complement(27421..27729)
                     /gene="lapA"
                     /locus_tag="ACHOQY_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9677791.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(27878..28642)
                     /gene="pgpB"
                     /locus_tag="ACHOQY_001668"
     CDS             complement(27878..28642)
                     /gene="pgpB"
                     /locus_tag="ACHOQY_001668"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9677792.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            28812..29402
                     /gene="ribA"
                     /locus_tag="ACHOQY_001669"
     CDS             28812..29402
                     /gene="ribA"
                     /locus_tag="ACHOQY_001669"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9677793.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(29466..32141)
                     /gene="acnA"
                     /locus_tag="ACHOQY_001670"
     CDS             complement(29466..32141)
                     /gene="acnA"
                     /locus_tag="ACHOQY_001670"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9677794.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(32305..32400)
                     /gene="ymiC"
                     /locus_tag="ACHOQY_001671"
     CDS             complement(32305..32400)
                     /gene="ymiC"
                     /locus_tag="ACHOQY_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9677795.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(32514..32681)
                     /gene="yciX"
                     /locus_tag="ACHOQY_001672"
     CDS             complement(32514..32681)
                     /gene="yciX"
                     /locus_tag="ACHOQY_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9677796.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(32684..32824)
                     /gene="ymiA"
                     /locus_tag="ACHOQY_001673"
     CDS             complement(32684..32824)
                     /gene="ymiA"
                     /locus_tag="ACHOQY_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9677797.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT         7775 a         8644 c         8407 g         8217 t
ORIGIN      
        1 ccaacagcaa catgccagga tgagttagcg aattacacta acaagtggcg aatttcatca
       61 tggcagaata caaagataat ttacttggtg aggcgaacag ctttctcgaa gtgctggaac
      121 aggtttcgca tctcgcaccg ctggacaaac cggtgctcat catcggcgaa cgcggcaccg
      181 gtaaagagct gattgccagc cgcctgcatt atctctcctc ccgttggcaa gggccgttta
      241 tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga ttccgaactg tttggtcacg
      301 aagcgggggc gtttaccggt gcgcaaaaac gtcatcctgg gagatttgaa cgtgccgacg
      361 gcggtacgct atttcttgat gaactcgcta cggcaccgat gatggtgcag gagaaattat
      421 tgcgcgtgat tgagtacggt gaactggagc gcgttggcgg cagccagcca ttgcaggtga
      481 atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc gatggtcaat gaaggcactt
      541 ttcgcgctga cctgctcgac cgactggctt ttgatgttgt acaactgcca ccactgcgcg
      601 agcgcgaaag cgacataatg ttgatggcag aacactttgc catccagatg tgtcgggaaa
      661 ttaagctgcc tctgttcccg gggtttacag agcgcgccag agaaacattg ctgaattatc
      721 gttggccggg aaatattcgt gaattgaaaa acgtggtgga acgttcagtg tatcgccacg
      781 gcaccagcga ttatccgctt gatgacatca ttattgatcc ctttaaacgg cgtccgcctg
      841 aagacgctat cgccgtttca gaaaccacct cgcttccaac actgccgctg gatttacgtg
      901 agtttcagat gcagcaggaa aaagagttgc tgcaactcag tttgcaacaa gggaaatata
      961 accagaagcg tgcggctgaa ttactggggt taacttatca tcagttccgc gcgttgttga
     1021 aaaagcacca gatttagcga agttgagcaa tacaacttgc cggatgcggc gtgaatgctt
     1081 tattcggcat acataatcaa tacgtcgttt gcaggcctga taagcgcagc gcatcagaca
     1141 ttattgcgtt gggctattaa tcgctcagcg catcctgcaa aattttcatt gccgcatcga
     1201 attgcgcatc cggtatggtc agcggataca ggaagcgaat cacgttgccg tatgcgccac
     1261 aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg
     1321 ctgacggctc gcccgtttgc ggatcgttaa actctgccgc aatcatcgac cccaggccgc
     1381 gtaccgcagc aatggccgga acgctttctt tggcatcaat caacgtgttt ttgagacgct
     1441 ggcccagttg attcgcgcgt tcgcagagtg attctttgtc gataatattg agcaccgcgt
     1501 gcgcggcagc caccgccagc gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg
     1561 gcgcgtccat aatattcgcg ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc
     1621 ttttcgccat cgtcattaag tccggcttat cggcgtaatg atccatggca aacagtttac
     1681 cggtacgcgc aaagccgctt tgcacttcat cggcaatcat cacaataccg tgctcgtcgc
     1741 acaggcggcg aatggcggca accagctctt ttggcgcaac gttgaaaccg ccctcgccct
     1801 gcaccggttc gaaaataatg gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc
     1861 gttcgatggc gtcgagggag tcctgtgttg aaatgccgtg taaatctgac ggataaggta
     1921 cgtgatacac cgaaccaggg aacgggccga agccgatttt gtacggcgca acttttccgg
     1981 tcagcgccat ggtcatatat gtacgaccgt ggaagccacc gctaaacgca atcacgccag
     2041 gtcgtccggt atgggcgcga gcaattttca ccgcgttttc caccgcttcc gcaccggtag
     2101 tgaagaatgc ggttttagcc tgcccgctca ctggggcaag ggcgttaatt ttctccgcca
     2161 gcgtgacgta gctttcgtac ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt
     2221 gctgctccac cgccgcgacc agatcagggt ggcgatgccc ggtattcagc accgcaatgc
     2281 ctgcggcgaa atcgatgtat tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg
     2341 actgggcgaa gaagttacac atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct
     2401 gatggaattc attgttgctc atgtttcaca ccttttgact cttcactgtt aatcggaaat
     2461 taaacgccca gacgatcgcg caggctgtaa taagccgcgc ccatcgcggt aaatggcacg
     2521 cgcagcgtgc gcccgccggg gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgt
     2581 tcggcgtcgc cgcgcagcag ttcggcaatc agacgcccgg caagatgggt acaggtgacg
     2641 ccgtggccgc tgtagccctg catgtaatag atgttggtat caaggcgacc gaactgcggc
     2701 atgcgcgaca aggtcagcag gaagttgccc gtccagcggt aatcaatttt cacgcccttc
     2761 agctgcggga aggttttcag cagtttcggc accacaaggc gctcaacgtc atccgggtcg
     2821 cgcgcgccgt agaccacgcc gccgccgtac agcaggcggt tgtcggcggt aagacggtag
     2881 taatcaagca gatagttaca gtcttccaca cagtagtttt tcgggatcag cgaacgggct
     2941 aaatcttccg acagccgttc ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc
     3001 gccagttccg gctctacttt atcgcccaga tacgcattcc cggcgacaat cacatacttc
     3061 gccgtcacct gacctttggc agttcgcaca acggctggcg tggtgtgctg gatctgcgtc
     3121 acggcagaaa gttcatacac gcgcccgccg ttgaggcgga tggcgtccgc ttcgccaatg
     3181 gcgaggttta gcggatgaat atgcccaccg ctgtgatcca gcagcgcacc ggtatagcga
     3241 tcgctggcta cttcacggcg aatggcgttg gcgtccagca attccagctg tttattgccg
     3301 tagcgttccc agttctcttt ctgctcttca agtgtggcga gctgtttatc attcatcgcc
     3361 acaaacaggc cgccggggcg gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg
     3421 atgatctcac caccttcgaa catcatgctg ccgagcatac gggcggtgtc catgccgtag
     3481 cttttttcga tcacgtcgat gtcgcggctg taggagttca caagctgccc gccattgcgc
     3541 ccgcttgcgc caaagccgat gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc
     3601 gccagatgca gcgcggaaga gagcccggta tagccgccgc cgaccacgca aacgtcgcag
     3661 gaaatcgact cattcagcgt gtcgaatggc gcgtatttat tcgcactggc ggcgtagtaa
     3721 ctgctggtat gttcggtcat ttcaggcctc caggcttatc cagatggttt tcagttcggt
     3781 gaatttttca agggcatgca gggatttgtc gcgaccgttg ccgctctgct tatagccgcc
     3841 aaacggcacg gtcatatcgc cgtcgttgta gttattgacg aagacggaac cagccttcag
     3901 gcggcggctc atgcggtgcg cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc
     3961 gtactggctg tcgttggcaa gctgtagcgc ctggtcttct gatgtgaaac gcgtgaccac
     4021 cagcaccgga ccgaaaatct cttcgcggct taaggacgca ttcgggtcta catccacaaa
     4081 gatggtcggg ccgatggcgg cagccagctc agcgttacga ccatccagca gcagttgccc
     4141 tttgctttcg ccttcccgaa taaagctatg gaccgagtca gcgtgagcgc tgtcgattaa
     4201 ggtgcccatg gtggttgcgg gatcaagtgg atggcccggc tgccagtttt gcgcctgctg
     4261 ttttaacagg gctaagaatt catcggcgat gctctcttcc agtaacaaac gcgttccggc
     4321 gatgcacacc tggccctggt tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg
     4381 cagatccgga caatcggcga aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac
     4441 gcgtttcatg ttgctctcgc ccgcatcttt cagcagctgt ttcccggtac gggtcgaacc
     4501 ggtaaaggca atggcgtcga tatcgttatg acgcgacagc gcctgccccg cttcatgacc
     4561 aaaacctgtc accacgttca aaacaccatc cggcaagcct gcttctttcg ccagccccgc
     4621 gagacgaatc gcactgagcg gtgatttttc agacggtttc aggacaacgc tgttccctgc
     4681 tgccagcgcc gggccaagct tccagcaggt cagcagcagc ggaaagttcc acggtacgat
     4741 ggcggcaatc acaccgaccg gttcacgcac gatcatcgcc agctcatggc tactggtggt
     4801 cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc
     4861 gccgggaata tcatcacgca gactgtgacg aatcggtttg ccggtgtcga gagtttccag
     4921 cagtgccagc tcttcggcgt gggcttccat taaatcggtg agtttattca gtaccgcttt
     4981 acgtttagcc ggagaagaga gtgaccagtc gccgcgttca aatacgccgc gtgctgcgct
     5041 caccgcacgg tcgatatcga cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt
     5101 gaccggatca acggtttcaa aggtttcatt ttccgccgca gcagtatatt caccgttaat
     5161 aaataagcgg gtttcaatgg cgagacttaa cgctttatcc tgccagtaag ccagatgatg
     5221 aaaattcatt atgactcctg tttcacgtct atcagatata tgcagtttgt gaacgtcagg
     5281 gcgcagagat ccccgggccg tatgcccgtt tatttctggc gacattcttt atatacactt
     5341 catccttcaa gccgcctctg ccttggctgc gttcgctccc ccgaatcact tacctgagta
     5401 aactcatcgg gattcgctca cttgccgcct tgatgcaact tgaatgattt tgtgtataga
     5461 ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg
     5521 tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc
     5581 cggtattaat ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca
     5641 gaacaatttc accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc
     5701 cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac
     5761 cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat
     5821 taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca
     5881 gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt
     5941 ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa
     6001 gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca
     6061 tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg
     6121 acaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca
     6181 ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac
     6241 taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg
     6301 cccatgcaga gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc
     6361 cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg
     6421 atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc
     6481 ggttgccacc accagttcct gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc
     6541 gagtgcggca ttaattaacg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc
     6601 atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat
     6661 gccatcgagt ttcggcaaaa gttgttcaag taatgacggt tccgccagcg catgtggtag
     6721 cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg
     6781 ggtcgcatga cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat
     6841 tatattttcc atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcggtttaa
     6901 atgaccttta tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca
     6961 atttaacatt tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt
     7021 acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt
     7081 tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga
     7141 agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga
     7201 tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agttggagaa
     7261 agggtgttat ttcccggcct cggtgtttgc aatggatatt ctgggcaacg tagtagaaga
     7321 agcgggtctg ggtcaggaaa tgggcgagcc ggatcgtact tgtgttcctg ttctcggttc
     7381 cttaacccct tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga
     7441 tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca
     7501 gctacgccag cgcggattgt tcccggttgt agcggtagag ctggagttct atttactgga
     7561 tcgtcagcgc gacgctgaag ggtatctgca accgccctgc gcgcccggca ccgatgaccg
     7621 caatacacaa agccaggttt actccgttga taacctcaac cacttcgccg atgtgctcaa
     7681 tgatattgat gaactggcgc agttacagtt aattcccgcc gacggtgcgg tcgccgaagc
     7741 ctcgccgggt cagtttgaaa tcaaccttta ccataccgat aacgtgctgg aagcctgcga
     7801 tgatgcgctg gcgctaaaac gcctggtgcg tctgatggca gaaaagcata agatgcacgc
     7861 cacttttatg gcgaagccgt atgaagagca cgcgggcagc ggaatgcata tccatatcag
     7921 tatgcaaaat aaccgtggcg agaacgtgct ttctgacgcg gaaggcgaag attcgccgct
     7981 gctgaaaaag atgctcgccg ggatgattga cctgatgccg tcgtcgatgg cgttgctggc
     8041 accaaacgtg aactcgtatc gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc
     8101 gtggggccat aacaaccgca ccgtcgccct gcgtattccg tgcggcgacc gtcataatca
     8161 ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca tatctggtga tggcagcgat
     8221 ttttgccggt attttgcatg gccttgataa cgagctgccg ttgcaggaag aagtcgaagg
     8281 caacgggctg gaacaggaag gcttaccctt cccgattcgc cagagcgatg ccctgggtga
     8341 gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca
     8401 cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc atcacagaaa cagaaattga
     8461 gtggatgttg aaaaacgcgt aattccccat gcggggttgt ttcatcggcc tgagcggagc
     8521 caggcctgac atttgctggt aatccagaca tttcccggat gtcgcgttat ggagcgcagg
     8581 cggcaacggg cttccgttaa cgattaagac tgtgacgagg aaatgagatg atgccgatga
     8641 ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc tcctgctact gactggactc
     8701 cgcccccttt gcttcccttg ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct
     8761 tattacgcag gggaggcagc aattatggct attaattcac cactgaatat tgctgcgcaa
     8821 cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggtggtaat gatgggtctg
     8881 gcctatctca cgccgatgac cgtatttgat acttttggta ttgtctccgg cattagcgac
     8941 ggtcacgttc cagcgtccta tttgctggcg ctggcgggcg tgctgtttac cgctatcagc
     9001 tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta cgcgcaaaag
     9061 tcgattaatc cgcacgtcgg atttatggtc ggctggtcat cactgctgga ttatctcttt
     9121 ttgccaatga tcaacgtttt gttggcgaaa atctatctct ccgccctctt cccggaagtg
     9181 ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc
     9241 gtcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc catcatggtg
     9301 gtgtttatct tcctggtggt tcagggactg cataaaggag aaggcgttgg caccgtctgg
     9361 tcacttcagc cgtttatcag cgagaacgcg cacctgatcc cgattattac cggtgcgacg
     9421 attgtctgtt tctcgttcct cggtttcgat gcggtgacca cgctttcgga agagacaccg
     9481 gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtatacgg cggcgttatc
     9541 tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg atatcagccg ctttaaagac
     9601 ccggacgccg cactgcctga aattgcgctg ttcgtcggcg gcaagctctt tcagtcgatt
     9661 ttcctctgca ccacgtttgt gaacacatta gcgtctggcc tggcttcaca tgccagcgtg
     9721 tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat
     9781 gtgcacccaa aatggcggac tccggcactc aacgtcatta tggtcgggat tgtcgcgctg
     9841 tcggcgctgt tctttgattt agtcaccgcg acagcactga ttaacttcgg tgctctggtg
     9901 gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat
     9961 aaaagctgga aggatcactt ccactatttg ctgatgccgc tggttggcgc gctgacggtg
    10021 ggtgtgctgt gggttaacct agagtcaacg tcactgacac tcggtctggt atgggcttcg
    10081 ttaggcggcg catatttgtg gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa
    10141 ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct
    10201 gagggtaacg cgccgcgcca ttaactataa aaagatgtat gacccggatc acgagtgcta
    10261 tcatccgccc ttatgcttag cgcattaacc ccaggggcaa ataatgaacc acgatatccc
    10321 gctaaaatat tttgatattg ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga
    10381 ggctgaacgc acaccgctgg cccactactt ccagttactg ctcacccgtt tgatgaacaa
    10441 tgaagagatc agcgaagaag cccaacatga aatggctgct gaagcgggga ttaatcccgt
    10501 acgcattgat gagatcgcag agtttttgaa tcaatggggt aatgagtaat tatttaattg
    10561 cagacgatcg gtcagtatcg ttgtccggat aaagcgttca cgccgcatcc ggctaacaat
    10621 gacacgcact gcagcgtgct ttaatgggtt tctcacgatc gtaccgctct gctcctcgct
    10681 aagttgttct gtgagttata gttatagaga aatcacgggt acgaatgttg aacagatggc
    10741 gacgccacct gttcaacgac caaccacacg gccctcatta cgacccctga acacaaacgg
    10801 taatggcaaa tgtcattaaa tattagtaag atagcctctt tacataaaat cccttaatat
    10861 tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg gtcaggcaat
    10921 cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg tctattgcgt
    10981 cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa ttgtcgatac
    11041 ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct atcgcctgat
    11101 gccggaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc gcttccacct
    11161 gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact cccactcgta aaatgaatgc
    11221 cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc aacaacccgt ggcataacgt
    11281 caacggcagc aacttcccct acttcgacag cctgcaattt gccgataacg tgaaaagcgt
    11341 ccgcaaactg gataatcata ccgttgagtt ccgactcgcc cagccggatg cttctttttt
    11401 gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc ggaagttaga
    11461 gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt
    11521 gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact tctggcgcgg
    11581 taaaccgtta atgccgcagg tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc
    11641 gaaactcctg accggggaat gcgacgttct ggcctggcct gctgccagcc agctatccat
    11701 tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct
    11761 ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg cactggcact
    11821 ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg aaacggcggc
    11881 ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta ctgaatacaa
    11941 tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg gaaaacttaa cgctgaaact
    12001 gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat
    12061 tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt
    12121 tcaggaggcg cggttgatgg atatgagcca tgatctgacg ttatccggtt gggcgacgga
    12181 cagtaacgac ccggacagtt tcttccgtcc tttactgagc tgcgcggcaa ttcattcaca
    12241 gaccaacctc gcccactggt gcgatccgaa attcgacagc gtgttgcgta aggcgctctc
    12301 ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa gcgcagagta ttctggcgca
    12361 ggaattgccc attttgccgc tggcgtcgtc attgcgtttg caggcctatc ggtacgatat
    12421 caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa
    12481 acaggatgag gtgaaaaaac catgattatc ttcaccttac gccgcatttt gttattgatt
    12541 gtcaccttgt tcctgctgac cttcgttggc ttcagcttga gctatttcac ccctcatgcg
    12601 ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaacgg cctgatccac
    12661 tgggattttg gtgtttccag cattaacggt caaccgattg ccgaacagtt aaaagaggtc
    12721 ttcccggcga cgatggaact gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc
    12781 cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt gatcaacgcc
    12841 atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt gacgctgttt
    12901 tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg
    12961 aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc atggcgggat
    13021 gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct gtcggttgcg
    13081 ccaacaactg aagtgatccg cctgatgcgt atcagcacta tcgaagtgta tgaccagaac
    13141 tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt
    13201 ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc caccatgctg
    13261 acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg ctggttaatt
    13321 aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt gtgtggctca
    13381 ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa ccctctgaaa
    13441 cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc cgggcacgct
    13501 gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc tgtacggctg
    13561 cgcggggctg gctgtactgt gtatttttgg cggctggttt gcgccttacg gcatcgacca
    13621 gcaatttctc ggttatcaat tattgccgcc gtcatggtcg cgctatggcg aagtttcttt
    13681 cttcctgggg actgacgacc tcggacgcga tgttttaagc cgtttactga gcggagctgc
    13741 gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg acgatctgtg gcctggtact
    13801 gggcaccttc gccggggcga cacacggcct ccgctcagcg gtgcttaacc atattctgga
    13861 taccttactg gcgatccctt cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc
    13921 gagtttgtcc cacgctatgt ttgctgtctg gctggcattg ctgccgcgta tggtacgttc
    13981 gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg cccgtctgga
    14041 tggcgcatca acgctgaata ttctctggtt tgctgtgatg ccaaacatca ccgccgggct
    14101 ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg cgctgggctt
    14161 tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct
    14221 ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa ttatgattag
    14281 cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga
    14341 ataatgccat tactcgatat tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg
    14401 gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt
    14461 ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga tttgtggggt gaataaagat
    14521 aactggcgtg ttactgctga ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc
    14581 gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga tgattttcca ggaaccgcag
    14641 tcgtgtcttg acccttcaga acgtgtgggt cgccagttga tgcaaaacat cccagcctgg
    14701 acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc cattgaactg
    14761 ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc gcagttttcc ctatgagttg
    14821 accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca accgcgtctg
    14881 ctgattgctg acgaaccgac caactcaatg gagccaacaa cccaggcgca aatctttcgc
    14941 ctgctgacgc gtctcaacca aaacagcaat accactattt tgcttatcag ccatgactta
    15001 caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca aacagtggaa
    15061 accgcgccga gtaaggagtt ggtgacgatg ccacatcatc cttataccca ggcgctgatc
    15121 cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa gtcgcctcaa tacgctgccc
    15181 ggcgctatcc cactgctgga acagttaccg attggttgtc gtctggggcc acgttgcccg
    15241 tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat
    15301 gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc tgcttgaagt
    15361 gcgtaattta agtaaaacgt tccgctaccg gaccggctgg tttcgtcgtc agaccgtaga
    15421 agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga ttattggcga
    15481 gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg ggaatgatag agcctaccag
    15541 cggcgagtta ttgattgacg atcatccact gcattttggc gattattcct tccgcagtca
    15601 gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc aacgtatctc
    15661 gcaaattctg gattttccac tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa
    15721 acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca gttactatcc
    15781 gcatatgttg gcacccggac aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg
    15841 cccaaaagtc attattgccg atgaagccct cgcttcactg gatatgtcga tgcgttcgca
    15901 gttgattaat ctgatgctgg agttacagga aaaacagggc atttcgtata tttatgttac
    15961 ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc atcagggcga
    16021 ggttgtcgaa cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa
    16081 acggctgatt gccgggcact ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg
    16141 ctaattttct gaatcctcct tccagattat ccacctcatt ctgacaagag attaaatctt
    16201 gtcagaatga ggtgatggat aaaataaaat caaatgcgcg tgatttaaga cgcaatctca
    16261 ctttgcagga acgaaagctc tggcgatatc ttcgcagccg acgttttggt gatttcaaat
    16321 ttcgccgtca acatccggtg gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg
    16381 tagtcgttga gctggatggt gggcagcatg atttggcagt tgcctatgat accaggcgca
    16441 ctagctggct tgagtcgcag agctggaccg tgctgcgttt ctggcataac gagattgatt
    16501 gtaatgagga ggcggtgctg gagattattc tgcaggaact gaaccgccgg tcaccctcac
    16561 cctgaaaggg cgagggggcc gaccgagccg aatagctgtt gtggtgaaaa catggagaca
    16621 gtgctggaga atattcggca aggtctgaac catcccagcc atcgccataa aagggttagg
    16681 ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatatgg tcccctcgcc
    16741 cctctgggga gagggttagg gtaaggggaa aagcgcccac cctgccgcag cctgctccgg
    16801 tcggacctgg caactatagc tactcacagc caggttgatt ataataaccg tttatctgtt
    16861 cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc atcaacaata
    16921 aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg tgttgccagc
    16981 aaactatcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc tgaactggca
    17041 ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc tcaattgggt
    17101 tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac catgttcgct
    17161 gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg ttttgcacct
    17221 ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaggctt caaaattgcc
    17281 cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc catgctgaat
    17341 ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat cccgaactac
    17401 aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg
    17461 atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg tactctggcg
    17521 gcctccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt
    17581 cgccgtaccg ttactattga agatgtgggt aactccgcgg cattcctgtg ctccgatctc
    17641 tctgccggta tctccggtga agtggtccac gttgacggcg gtttcagcat cgctgcaatg
    17701 aacgaactcg aattgaaata atcattctgt tggtaaatat tgggcggcgt tctgccgccc
    17761 gttatctctg ttataccttt ctgatatttg ttatcgccga tccgtctttc tccccttccc
    17821 gccttgcgtc aggataacga tttcctttac gaccaaggag cgcccatgga acaacgccac
    17881 atcaccggca aaagccactg gtatcatgaa acgcaatcca gtactgcgga gtatgacgtt
    17941 ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct ttctgctcga cgtgatcctt
    18001 gatgaagaaa cgctggctcc cttcctttca tggctggtcc ctgcgcgcgt tcttgcagtg
    18061 gaattgttcc ctgaccagct taccgtgacc cgttcacaga ccttcaccgc ttatgaacgc
    18121 ttgtctacgg ccctgacggt tgctcaggtt tgcggcgtcc agcggttatg taactactat
    18181 tcggcgcgac ttacgccgct ccccgggcct gattcctcca gggaaagcaa tcataggttg
    18241 gcacaaatca cgcaatatgc ccgccaactg gctagctcgc cttctattat cgacaaccga
    18301 tcgcgccagc atctgaatga cgtcggtctt actgccgggg actgtgtgat cattaaccaa
    18361 atcattggtt ttattggctt tcaggcgcgg accattgcga catttcaggc ttatctcggg
    18421 cacccggtac gctggttacc cggtctggaa atacaaaact acgccgacgc gtcactgttt
    18481 gctgatgaat caatacgctg gcgaagcagc tatgaggtgg aaaaactacc tgaagagtac
    18541 acaaaaagtt caacagcaga actttgccaa ctggccgaaa cactctctct ctccacccta
    18601 tttcactttc ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata
    18661 ataagcttgc ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct
    18721 gtatggattc atcaaatgaa tataaaaaaa tcagccccct tctgcgcaag ggcgaaaatg
    18781 aaattaacca atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat
    18841 ggctgacccg agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcgaacacg
    18901 aaaaatcatc aacgcagatt attaatctgc tggtatggag cgggctgtat ggctggataa
    18961 atcgcttaaa aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg
    19021 taaaactgtc agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg
    19081 ctgctagcgc agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta
    19141 aaagccacag aaaaaggctt tggcttcctg gaagtcgacg cgcaaaaaag ttatttcatt
    19201 ccgccgccgc agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt
    19261 gaaaaagaac gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc
    19321 gtgggtaaag ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta
    19381 aaagacgcca ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac
    19441 tgggcggttg ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa
    19501 ctgacacaat acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg
    19561 cgccataatc tggaaaaaga agcaccagac ggcgtcgcta ccgcaatgct cgatgaaggt
    19621 ctggttcgtg aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa
    19681 gatatggatg acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg
    19741 gcgattgccg atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa
    19801 attcgcgcat tcaccaacta tctgcctggc ttcaacatcc ctatgctgcc tcgcgagctt
    19861 tctgacgatc tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg
    19921 acgctctccg ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa
    19981 tccaaagcaa agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg
    20041 cagcctgaaa gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc
    20101 cgcggcgagt ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt
    20161 attctcggtg aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac
    20221 cgtatcgtcg aagaagcgat gattgccgct aacatttgtg cggcccgcgt actgcgcgat
    20281 aagctcggtt ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg
    20341 ctggcagcgt tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg
    20401 gacggtttct gcaaactgcg tcgtgaactg gacgcgcaac caactggttt cctcgacagc
    20461 cgcattcgtc gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc
    20521 ctcggtctgg aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc
    20581 aaccaccgtc tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgccc acaggatgaa
    20641 atcactgtcc aaatggccga gcgtcgccgt ctcaaccgga tggcagaacg tgatgttggt
    20701 gactggttat acgcacgctt cctgaaagac aaagccggga ccgacacccg tttcgcagcg
    20761 gaaattgtcg atatcagccg tggcggcatg cgtgttcgtt tggttgataa cggcgctatc
    20821 gcctttattc cggcaccttt cttacacgct atgcgcgatg aactggtttg cagccaggaa
    20881 aacggcactg tacaaattaa aggtgaaacg gtttacaaag tgactgacgt tattgacgtc
    20941 actattgccg aagtccgcat ggaaacccgc agcattattg cgcgcccggt cgcgtaatct
    21001 cctttcacgg cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata
    21061 tccaagcaga ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt
    21121 acaaataaat atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt
    21181 tgttcctttt atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc
    21241 gttagggagt ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg
    21301 acggaaagca gcgatgtttt gcgcttttct accaccgaaa ccacagaacc tgatcgtaca
    21361 ttgcagttat ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc
    21421 ctgatgatga gtctgaccgt cgatgaaagc gatctttctg tgcatctggt aggacgaaaa
    21481 atcaataaac gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcggttgct
    21541 cgtgatttat cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca
    21601 atagtgattc tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac
    21661 acagggttga aagaacacga cgtcattggg caaagcgtgt ttaaactgtt tatgagccgt
    21721 cgtgaagctg cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat
    21781 gaagtcgaac tgtggatacc aacatgtaaa ggccagcggc tgtttctgtt tcgcaataaa
    21841 tttgtccaca gcggcagtgg caaaaacgag atttttttaa tctgttccgg caccgacatt
    21901 accgaagagc gccgcgctca ggagcgactg cgtattctgg caaataccga cagtatcacc
    21961 ggactgccga atcgtaacgc aatgcaggat ttaatcgatc acgctattaa tcatgcagat
    22021 aacaataaag ttggggttgt gtatcttgat ttggataatt tcaaaaaggt caacgacgcc
    22081 tatgggcatt tgtttggtga ccagttatta cgcgacgtgt cattggctat tttaagctgt
    22141 ctcgaacatg accaggtgtt ggcgcgtcca ggtggggatg agtttctggt actggcatcc
    22201 aacacctcac aaagcgcgct ggaagcaatg gcatcacgaa ttttgacccg cttacggctc
    22261 ccctttcgca ttggtttaat tgaagtttat accagctgtt cagtaggtat tgcactctct
    22321 cccgaacatg gttcagacag cacggctatt attcgtcacg ccgacacagc aatgtacaca
    22381 gcgaaggaag gcggacgagg acaattttgc gtttttaccc cagaaatgaa tcaacgggta
    22441 tttgaatatc tctggctgga taccaacttg cgtaaagcac tggaaaacga tcagttggtt
    22501 attcactatc aaccgaaaat cacctggcgt ggcgaagtgc gcagtctgga agcactagta
    22561 cgttggcagt cacctgaacg tgggttgatt ccaccgttgg acttcatttc ctacgccgaa
    22621 gagtcagggc taattgtgcc tttaggccgt tgggtgattc tcgatgtcgt acgccaggtg
    22681 gcaaagtggc gggaaaaagg cataaacctg cgagtggcgg taaatatttc tgcacgtcag
    22741 ctcgccgatc aaaccatttt caccgccctg aaacaggttc tccaggaact caattttgaa
    22801 tactgcccta tagatgttga actgacagag agttgtctga ttgagaatga tgaactggca
    22861 ctgtctgtta ttcaacaatt tagccaacta ggtgcgcaag tgcatctgga tgattttggt
    22921 accggctact cttcactttc gcaactggcg cgctttccga tcgatgccat caaacttgac
    22981 caggtttttg ttcgagatat tcacaaacaa cctgtctcgc agtcactggt ccgggcgatc
    23041 gtcgctgtgg cccaggcatt gaatcttcag gtgatcgccg aaggtgtaga gagtgcaaag
    23101 gaagatgctt ttttaaccaa gaacgggatc aatgagcggc aaggattttt gtttgccaaa
    23161 ccgatgcccg ccgtcgcctt cgaacgctgg tataaacgct atctgaagcg cgcataagta
    23221 gatgaatata tatacttagc gctatatccc gtcattatat atcgtattcc tggatgataa
    23281 ttagccgttg ctgttaaaca cttctctttt agcggcaatt tttgcattat tcattttccc
    23341 ccttttgcta taaggatgtc cttgatgtct gaatttgatg cccagcgcgt tgccgaacgt
    23401 attgatattg tgttagatat ccttgtggca ggcgattacc actctgcgat ccataatctc
    23461 gagatactta aagcagaact cttgcgccag gttgctgaat ctacgccaga catcccgaag
    23521 gcaccgtggg agatttaaca atcgtgcctt caggttttcc tatttgagga tgcccggcgc
    23581 gtcagtattt ttttatttag tattataacg ttataagaat tacagcgatg aattcccgac
    23641 aacaaactat tctacagatg gtcattgacc agggtcaggt tagcgtaacc gatctggcaa
    23701 aagccactgg agtttctgaa gttaccattc gccaggatct caacaccctc gaaaaactga
    23761 gttacctccg ccgtgcacat ggctttgcag tttcgcttga tagtgatgac gtcgaaaccc
    23821 gtatgatgag caactatacg ctgaagcgtg aactcgccga gtttgccgcg tctctggttc
    23881 aaccgggcga aaccatcttt atcgaaaatg gtagcagtaa tgccctgctc gcccggactc
    23941 tgggcgaaca gaagaaaaat gtcactatca tcacagtcag cagctacatt gcgcatttgc
    24001 taaaagacgc gccttgtgaa gtcattttgc tcggtggcgt gtaccagaaa aaaagcgaaa
    24061 gtatggttgg ccctttgaca cgccagtgca tccagcaggt acatttcagc aaagcattta
    24121 ttggtattga tggctggcaa cctgaaactg gattcaccgg tcgcgatatg atgcgtaccg
    24181 atgtggtcaa tgccgtgctg gaaaaagagt gcgaagcgat agtcctgact gacagctcga
    24241 aatttggtgc tgtacattca tactccatcg gtcccgttga gcgattcaat cgcgtgatta
    24301 ccgattcgaa aatacgcgcc agcgatctga tgcatcttga gcacagtaaa ctcaccgttc
    24361 acgtcgttga catttaacca tttcccttct ctacggatga tttgcagttt ggcaaatcat
    24421 ccgctctaag atgattcctg gttgataatt aagactattt acctgttatt aacacgctta
    24481 agatataaaa ttattatcag cgatataaca ggaagtcatt atcacctgcg tgatataacc
    24541 ctgcgcgcga gcagatttca cggaataatt tcaccaggct tattcttagc tattatagtt
    24601 atagagagct tacttccgtg aatcataaat tcaggagaga gtattatgtt tgtaacgagc
    24661 aaaaaaatga ccgcggctgt tctggcaatt actttggcaa tgtctctgag tgcctgttct
    24721 aactggtcta aacgggaccg caacaccgca atcggcgcgg gtgcaggggc attaggcggt
    24781 gcagtactga ccgatggcag tacgttgggt acattaggtg gtgcagctgt cggtggtgtt
    24841 attggtcacc aggtcggtaa ataatcgtaa ataaatgtcg attatcgact cgcaattata
    24901 atacgcttga ttctgttcag tacgaaataa ctgaataata cacacagtaa aatattcgaa
    24961 agccacggat atatccgtgg ctttttatgt taaccgcctg cgagttttac cttcatccct
    25021 ttcgcttcaa gtagtgattt taataaatca cgcttatcgc cctggatttc aataactcca
    25081 tctttaactg ctccgccgca gccgcatttt ttcttcagtt ccgctgcaag ttttgtcagt
    25141 tcggcatcat cgagatcgac accggtaatc aggcaaacgc ccttaccttt acgtccactg
    25201 gtctgacgct gaatacgcac cacaccgtcg cctttagggc gtacaggggc cgctttgggc
    25261 tcatcaatac gtccggtttc cgttgagtag accagacggc tgttggaatc actcatcatg
    25321 cactccgctg taaagaggcg ttgatcgctt tcagcgtctg cgctggatct accgattgcg
    25381 ttaccgggcg accaatcacc atataatcaa caccagccga caacgcctgt tctggcgtca
    25441 taatgcggcg ctggtcacca gcttcactcc cctgcggacg aatgcccggc gtaaccagtt
    25501 tgaactcctg accgaatacc tgtttaaagc gcacagcttc ctgagcagaa cacaccacac
    25561 catcaaggcc acatttttgc gtcagtgccg ccagacgttc tgcataatct gcaggtgaca
    25621 gtgtcatgcc aagatcgacc aggtcgctgg cttccatgct ggtcaacact gtcacagcaa
    25681 tcaaaagcgg tgcatctttg ccaaacggaa ccagtgcctc acgcgctgcg gtcatcatac
    25741 gcgccccacc agaggcatga acattcacca tccacacgcc taagtcagct gcagcagcga
    25801 cagcgtgcgc agcagtgtta ggaatatcgt ggaatttcag gtcaagaaag atatcaaaac
    25861 cacgctgttg aagttcgcgc acaaactgtg gcccaaacaa tgtaaacatc tctttgccga
    25921 ccttcagacg acaatcgcgt gggtcgatct tgtcgacaaa ggacagcgcg tcatcacgat
    25981 tatgataatc aagggcaaca accacaggag aattcgtaac agcgcgggaa gaagatgaag
    26041 cagttaacgt catgaccaga ccttcttgat gatgggcacc ggaagtggcg cgaaacaggt
    26101 aaacggcgag cattctaccc gtggaaataa aaaattaaca gttgcgattt ttcccctggc
    26161 aggctttttc tgcgccgtcg ttgccggagc acaatggaac atcaataact agtatgttgt
    26221 aactaaagta ggatttttta aaaattacag gccatcaaga ccgcgaatcg gtttaatggt
    26281 tgaccaggcc cgacaagacg gacaatgcca gtagagggtg tatgcggtaa aaccacattt
    26341 ctggcagcga taacgaggct tactacgtac cttttcgcca accatgtcac gcagcaccat
    26401 caggctctct ttggcacgcc cttcttccgc ttcatttaag tggtaatcca ttaacttatg
    26461 gaacacacgc atggtcggat gacgctgaag ctggcgcgta atatagacct gtgcggcctc
    26521 actaccgtcg cgcgcttcga tgatatcagc aagcatcaat tcagcatcgg caccggtgtt
    26581 ctcttccacc gcgcgttgca ggaattctgc ccattcggca gttttaccca actgctgata
    26641 gcaggtttgc aacatttcca gcgtttcgct gaccagttct ctgtcctggg atatcacgcg
    26701 ttgcaaactt tcgacggctt tggcgtattc tcctttcgcc ataaacacgc gtcccatcat
    26761 gatggatacg cgggcgctgt ttttatctgc cgccgcccct ttttttagca aggtcatggc
    26821 acgatcgaga tcgtcgctgg ccatatgctg cagggctaac tcacagtaga aatgggcaat
    26881 ttcgacgcgc tgtttatctt tacccagctt caccaggcgt tcggcaacat caattgcttt
    26941 ctgccactca ctggtagcct ggtagatttg tagcaactgt tgcagagcgc caatgcggaa
    27001 gtcagtttca tcggtcagct gattgaacat gtcttccgcg cggtcgtata acccggcggc
    27061 catgtaatca cgccctagtt gttgaatcgc caacagacgc tgttcatagg tcagcgaggc
    27121 gctttccatt agggtctgat ggatgcgaat agcgcgatca acttcgccac gcgaacggaa
    27181 caggtttccg agcgtaaggt gggcttcaac ggtgcctgta tcctctttaa gcatatcgag
    27241 aaacaggtct accgctttat cctgttgatt actaagcagg aagttaaccc ccgctacgta
    27301 atcacgcgac aagcggttgg cttcatcttg cttgttttgt tgcgcacttc tgcggcccat
    27361 ataccagcca taggcagcgg ctacaggcaa aagcagaaac aacaactcca gcatagaaag
    27421 ttattccttc gccgctgacg agtgcggcac tacagccacg tcagtcgcgg gtgaaagctg
    27481 gttttccagt cgctttattt tacgttcagc gcgcgccagg gaaacacgaa ctcgcagcca
    27541 gaacaggcca caaatcaacc aaccgatagc aaaccccgca gcaaacaata ccgccagcaa
    27601 tgtggaaata cggtactccc cttgcgctaa cagataatta aacgtcacct gttgatcgtt
    27661 ctgcgcaccc aacgtgaccg aaatcacgaa gatcgctaac accagtaaga aaatgagtaa
    27721 atatttcaca ttacttcccg ttatgtggtt agagcgaata aattatggtc aacttaccgc
    27781 aatagccctt taaagtacca ttttcgcgcc gtctgcgaaa tggcatccac gcgcattgtt
    27841 gatgatttaa gggctaaatt cggaaaatca accagcgtta actttcttgt tctcgttgcg
    27901 ctatttcgcg attttcttcc gcaggtggtg ttaatggccc acaaattcgt tgcgcaagcc
    27961 acgttgccac cgccaccagc gcccacgaaa tcaacgtagc tactaccaga tcgcgtggcc
    28021 aatgcatccc gagcagcagg cggcttccca tgactcccgt tgcccagacc agcaagatag
    28081 caatggttaa cgttcgccga cgcggccaca gcaaaccaac ggccagcagt gcccaactgg
    28141 cagcaaacat cgtgtgaccg gaaggaaagg caaaccccgt ctctttctgc caatgtgaac
    28201 gcaaatattg tgggatattt ttctcttcag ccaactgttc tttcactaga tttccgcgtt
    28261 ctgctcgctt taaagtgtag aactcatcaa ccggaatatg atgtgttttt tccagccaga
    28321 taacaaaagg tcgtggttcc tggactttgt ctttgatcca ggatttaacg ccttgtccca
    28381 caaggattgc ggccgccaga atggcaaata atacaaaggc agccttaatg cgaaaacgca
    28441 gacaccagag aaaccagccg aataaaatca aatgtgtaat gacgccccag ggctgggtga
    28501 cagtttcagt aacccaaaaa gccgctttta gtagccaact ttgttctcca ggttgccaac
    28561 gccagccaga aatccatacg gctactggca tgacaagcaa tagtgcagct cccactgcgg
    28621 tacgtctggc aatcgaacgc atggcctctc ctttttgata agtcccacaa tcataactga
    28681 aaacgccagt tccaggaaaa attgacagat ttgtgccatt ccgtgaacga tcgacgcgtc
    28741 gtgattaggt gaaccccttc tcgttatggc aaaataagcc aatacagaac cagcattatc
    28801 tggagaattt catgcagctt aaacgtgtgg cagaagccaa actgccaacc ccatggggcg
    28861 atttcctgat ggtgggattt gaagaactgg caaccggaca cgatcatgtc gcgctagtct
    28921 atggcgatat ttccgggcat accccggtac ttgcgcgcgt ccattccgaa tgtctgaccg
    28981 gtgacgccct gttcagcttg cgctgcgatt gtggcttcca gctcgaagcg gcattgacgc
    29041 aaattgccga ggaaggccgt ggtattttgc tgtatcaccg tcaggaaggt cgtaacattg
    29101 gtctgctgaa taaaatccgc gcttacgcac tgcaggatca aggttacgat accgtagagg
    29161 ctaaccacca gttaggcttc gccgctgatg agcgcgactt cactctttgc gctgatatgt
    29221 tcaaactcct tggcgtcaat gaagtccgct tgttaaccaa taacccgaaa aaagtcgaaa
    29281 ttctgaccga agcagggatt aatattgttg aacgcgtacc attgattgta ggtcgtaacc
    29341 ccaataacga acattatctc gataccaaag ccgagaaaat gggccatttg ctgaacaaat
    29401 aaccctcttg cattgtgtaa ttaatttgct tgccggaagc aaaataaccg gcaagcaaat
    29461 acttgttact tcaacatatt acgaatgaca taatgcaaaa tgccgtcgtt ctggtagtag
    29521 gtcaactccg tcgcggtgtc gatacgacaa cggcagggta cgacctcctg gctaccatcc
    29581 gcgcgcgtaa gcgtcaccgg aaccgtcgca ccgggttgta gattttgcag atcgccaata
    29641 tcaatcttct cttccccggt tagtcctaac gttttacgcg ttacgccttg cggaaattcc
    29701 agcggcagga tgcccatgcc aattaaattc gaacggtgaa ttcgttcaaa cgattcggca
    29761 atcaccacac gaattccaag cagacgagga ccttttgccg cccagtcacg gcttgagcct
    29821 gatccatact ctttcccggc aatcaccgcc agcggcgttt gttccttctt atagcgcatc
    29881 gcagcatcat aaatagagac tacgtcgctg tcaggtaaat gccgcgtcat ccccccttca
    29941 acgccaggca ccatttcatt acggatgcga atattggcga aggtgccgcg catcatcact
    30001 tcatggttac cacgccgcga accgtaggag ttaaagtctt ttcgctcaac accccgacct
    30061 tgtagatatc gacccgctgg gctgtcgggc ttaatactgc ccgccggaga gatatggtca
    30121 gtggtgactg aatcccccag cattgcgagg atccgcgcac cgtgaatatc ttccactggt
    30181 gctggtgttg cctgcatttc atcaaagaaa ggcgataagc gaatataggt tgagtcctcc
    30241 tgccaaccgt aggtatcgga tcgtgtgacg ttaatttcct tccactctgc tgtgccttca
    30301 aaaacttctg cgtactcttt gcggaacatt tctgtggaga cttgttctac cgcacgggca
    30361 atttcttgtg ccgatggcca gatatctttc agataaaccg ggtcgccttt acgatcatga
    30421 ccaataggct cagcagccag gttgatattc atatttcccg ccagcgcata ggcaaccacc
    30481 agcggcggcg aggccagcca gttagtttta accagcggat ggatacggcc ttcaaagtta
    30541 cggttgccgg acagcaccgc accgacggtt aaatcgcctt ttttgattgc cgtttcgata
    30601 ggatcgggca gcggcccaga gttaccaata caggtggtac aaccgtatcc cacaaggtta
    30661 aaccccagtt cgtcgagata cggtgtcagt ttcgcttttg ccagataatc agaaacgact
    30721 ttcgaacccg gtgccagcga cgctttgacc cacggttgcc gcttgaggcc cagagttacg
    30781 gcttttttcg ccagcaagcc tgcggccatc agcacacttg ggttagaggt gttggtgcac
    30841 gaggttatcg cagcaatgac cacagcgcca tcaggtaact gatactgatg tccgttcata
    30901 acataatcga ccggctggcg atctttatgc gtggcattca cttccagttc gttactggcg
    30961 gcaaatgctt ttggtacatc gggcagtgca acgcgatcct gtgggcgttt tggccctgcc
    31021 aggctcgctt caacgtcatt catatccagt tctaacgtac tggtaaaaat cggttcatcg
    31081 cccgggttac gccacatgcc ctgcgctttg gcatattttt cgaccaactc aacctgatct
    31141 tcgctgcgcc cgcttaaacg catgtaatcg agggttacag catcgattgg gaagaagcca
    31201 caggtggcac catattctgg cgacatattg gcaatggtgg cgcgatccgc cagcggtagt
    31261 gaatccagac catcaccata aaattcgacg aatttcccca ccacgccatg tttgcgcagc
    31321 atttgggtaa cagtgagaac caggtctgtg gcggtaatac cttcacgtaa tttaccggta
    31381 agtttaaagc ccactacgtc cgggataagc atggaaaccg gctggcctaa cattgcggct
    31441 tctgcttcga tcccacccac gccccacccc agcacgccaa ggccgttgat catagtggtg
    31501 tgcgagtcag taccaacgag tgtatccgga taagcaatcc attcaccgtc ctgcaattca
    31561 ctccacactg ctttgccgag atattcgagg ttaacctgat ggcaaatgcc tgtgcctggc
    31621 ggcacgacgc taaaccgact gaacgcttgc tttccccatt tcaggaacac ataacgttcg
    31681 tggttgcgct ccatttccag gcgtacgttt tcttcaaatg cctcatcatc accaaaacga
    31741 tcgacggtca ccgagtggtc aatgaccagg tcgaccggtg agagcgggtt aacctttgca
    31801 gtatcgccgc cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac aacggcaggt
    31861 acgccggtaa agtcctgcat cagcaccctt gccgggcggt aggcaatttc acggtcagca
    31921 tgggcatttt tcagccatcc tgccagcgcg tggatatcct cttcggtaac cgagttacca
    31981 tcctgccagc gcagcaggtt ttcgagcaaa actttgagtg acttgggtag acgggtgata
    32041 tcgcccagtg atttagcagc aagcggcagg ctgtagtagt ggtaagtttt atctttggcc
    32101 tgcaacgtgt ccttactggc ttctcgtagg gttgacgaca tagctcctcc ttaatgacag
    32161 ggttgcgaag ccctgattct cttcagggtg aatattaaag ataacacaaa catcgcgtaa
    32221 cgatttgata acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa gcaaatgaca
    32281 accccgccgc agcggggtca aggatcagtg agtaatgatc cagaggagtt cggcccagaa
    32341 gagcatcgac agagaaaacg cgcccatcca ggaccaatat ttcatatttg tgttgatcat
    32401 aggctacacc cttaacggtt attattttgt gttgagataa ctcaagctgt tattgctgcg
    32461 gtgtattaaa agccctttaa ttcattaaaa atgggtaata agaatgatga taattattgt
    32521 ttcggctgat catcttcagc aaacagttct ataaaggctt gctgctgcga tgttagttgc
    32581 cagtcgtctg gaataacgac ggaacattcc cgtttcgttt ctgtttcatt gtcactatgc
    32641 tggcataatg gtgacgactc cagttgctcc tgaccgacca tagttatccc cacactttcc
    32701 aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc gccagccagg
    32761 ctttacgttt taattcagga tcgcggcggg gttcctgatt tccagaaggc attgctaacc
    32821 tcatataatc gccatcactt atcagcaaga cgttgaacga ttggtgtaat taatcatacg
    32881 atgagacaaa tcctaaagaa actttagtca aaaatccagc gaatttacga atcgtctcca
    32941 gtgaataatt caatcttttg acctgaaata gatatttgaa aactggagtt tgcgaggcgg
    33001 gtaattagac actactttac aggtaattta ccctataaat atg
//
LOCUS       DAZKGS010000035        32705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKGS010000035 DAZKGS010000000
VERSION     DAZKGS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHOQY_001674"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHOQY_001674"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9677798.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHOQY_001675"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHOQY_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9677799.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHOQY_001676"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHOQY_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9677800.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHOQY_001677"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHOQY_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9677801.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="ACHOQY_001678"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="ACHOQY_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP9677802.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..7374
                     /gene="bcsA"
                     /locus_tag="ACHOQY_001679"
     CDS             4756..7374
                     /gene="bcsA"
                     /locus_tag="ACHOQY_001679"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9677803.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7385..9724
                     /gene="bcsB"
                     /locus_tag="ACHOQY_001680"
     CDS             7385..9724
                     /gene="bcsB"
                     /locus_tag="ACHOQY_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9677804.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9731..10837
                     /gene="bcsZ"
                     /locus_tag="ACHOQY_001681"
     CDS             9731..10837
                     /gene="bcsZ"
                     /locus_tag="ACHOQY_001681"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9677805.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10819..14292
                     /gene="bcsC"
                     /locus_tag="ACHOQY_001682"
     CDS             10819..14292
                     /gene="bcsC"
                     /locus_tag="ACHOQY_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP9677806.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14374..16362
                     /gene="hmsP"
                     /locus_tag="ACHOQY_001683"
     CDS             14374..16362
                     /gene="hmsP"
                     /locus_tag="ACHOQY_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9677807.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16545..17831
                     /gene="dctA"
                     /locus_tag="ACHOQY_001684"
     CDS             16545..17831
                     /gene="dctA"
                     /locus_tag="ACHOQY_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9677808.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18051..19547
                     /locus_tag="ACHOQY_001685"
     CDS             18051..19547
                     /locus_tag="ACHOQY_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9677809.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19643..20572)
                     /gene="kdgK"
                     /locus_tag="ACHOQY_001686"
     CDS             complement(19643..20572)
                     /gene="kdgK"
                     /locus_tag="ACHOQY_001686"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9677810.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20804..21571
                     /gene="pdeH"
                     /locus_tag="ACHOQY_001687"
     CDS             20804..21571
                     /gene="pdeH"
                     /locus_tag="ACHOQY_001687"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9677811.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21641..23701
                     /locus_tag="ACHOQY_001688"
     CDS             21641..23701
                     /locus_tag="ACHOQY_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9677812.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23935..25257)
                     /locus_tag="ACHOQY_001689"
     CDS             complement(23935..25257)
                     /locus_tag="ACHOQY_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9677813.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25668..26681)
                     /gene="yhjD"
                     /locus_tag="ACHOQY_001690"
     CDS             complement(25668..26681)
                     /gene="yhjD"
                     /locus_tag="ACHOQY_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9677814.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26730..27629)
                     /locus_tag="ACHOQY_001691"
     CDS             complement(26730..27629)
                     /locus_tag="ACHOQY_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9677815.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            28149..28751
                     /locus_tag="ACHOQY_001692"
     CDS             28149..28751
                     /locus_tag="ACHOQY_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9677816.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28802..30451)
                     /locus_tag="ACHOQY_001693"
     CDS             complement(28802..30451)
                     /locus_tag="ACHOQY_001693"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9677817.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30856..32253
                     /gene="ccp"
                     /locus_tag="ACHOQY_001694"
     CDS             30856..32253
                     /gene="ccp"
                     /locus_tag="ACHOQY_001694"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9677818.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            32464..>32705
                     /locus_tag="ACHOQY_001695"
     CDS             32464..>32705
                     /locus_tag="ACHOQY_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9677819.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         7803 a         8230 c         8946 g         7726 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggcta tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gttttgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcactcacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ctagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcattattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgttctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc actcgctggc
     4441 tatcgtcaat gtggatgcca actgccatat ccgcctgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaata acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattgct gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt cggtgaatac cgcagtgatg cgctggcggc
     4681 agaagagata ctgacgctgg cgaactggtg cctgttgaac tattccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgccacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacttg tatccgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg caggcgggca ttttcaggtc tgcaaaatat tcgtggacgt
     5101 taccatcaat ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatctggat
     5161 gagaaaaaag agctcggtca tttgagtgcc ggggcgcggc ggttgatcct cggtatcatc
     5221 gtcaccttct cgctgattct ggcgttaatc tgcgttactc agccgtttaa cccgctggcg
     5281 cagtttatct tcctgatgct gctgtggggg gtagcgctga tcgtacggcg gatgccgggg
     5341 cgcttctcgg cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg ttatatctgg
     5401 tggcgttaca cctctacgct gaactgggac gatccggtca gcctggtgtg cgggcttatt
     5461 ctgctcttcg ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt ccaggtagta
     5521 tggccgctga atcgtcagcc ggtgccattg ccgaaagata tgtcgctgtg gccgtcggtg
     5581 gatatctttg tcccgactta caacgaagat ctcaacgtgg tgaaaaatac tatttacgcc
     5641 tcgctgggta tcgactggcc gaaagacaag ctgaacatct ggatcctcga tgacggcggt
     5701 agggaagagt ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgtaccact
     5761 catgaacatg cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc
     5821 gtgtcgattt tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc
     5881 tggttcctga aagagaaaca gctggcgatg atgcagacgc cgcaccactt cttctcgccg
     5941 gacccgtttg aacgcaacct ggggcgtttt cgtaaaacgc cgaacgaagg cacgctgttc
     6001 tatggtctgg tgcaggatgg caacgatatg tgggacgcca ctttcttctg cggttcctgt
     6061 gcagtaattc gtcgcaaacc attggatgaa attggcggta ttgctgtcga aacggtaacc
     6121 gaagatgcgc acacgtctct gcgcctgcac cgtcgtggct acacctctgc atatatgcgt
     6181 attccgcagg cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt
     6241 cgctgggcac gcgggatggt gcaaatcttc cgtctcgata acccgcttac cggtaaaggg
     6301 ctgaaattcg cccagcggct gtgctacgtc aacgccatgt tccacttctt gtcgggcatt
     6361 ccacgactga tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc
     6421 tatgcgccag cgttgatgat cgccctgttc gtgctgccgc atatgatcca tgccagcctg
     6481 accaactcca agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg
     6541 gtgctggcgt ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc
     6601 aaatttaacg tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc
     6661 tcgcggccct acatcttcct tgtcctgctc aacctggtgg gcgttgcggt aggcatctgg
     6721 cgctacttct atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggta
     6781 ttctacaacc tgattgttct tggcggcgca gttgcagtat cggtagaaag caaacaggta
     6841 cgccgatcgc accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga agatggtcac
     6901 ctcttctcgt gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt
     6961 caggcgcaga ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa
     7021 tacgtcttcc cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct gaaattaatg
     7081 ccgctcacca cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca
     7141 tgggcgctct ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt
     7201 ctgaagctcg gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc ttcggtgaag
     7261 ggcatattcc gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc
     7321 ccggagcgga gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa
     7381 cgcgatgaaa agaaaactat tctggatttg tgcagtggct atggggatga gtgcgttccc
     7441 ctctttcatg acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt
     7501 agccgcccag acggaacaaa atccgcaggt ggggcaagtg atgccgggcg tgcagggcgc
     7561 tgatgcgcca gtcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga cctttgcgca
     7621 aattgcgccg ccgccgggca gcatggtgct acgtggcatt aacccgaacg gcagcattga
     7681 gtttggcatg cgcagcgatg aagtggtgac gaaggcgatg ctcaacctcg aatacacgcc
     7741 atcgccatcg ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgagctgat
     7801 gggcgtgctg ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc aaatgcccat
     7861 taacccactg tttattaccg acttcaaccg tgtgcggctg gagtttgtcg gccattatca
     7921 ggacgtgtgc gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg
     7981 actggatctg acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc
     8041 attctttgac ccgcgcgata accgtactaa caccttgccg atggtctttg cgggtgcgcc
     8101 ggatgttggg ctgcaacaag cctctgccat tgtcgcctcg tggtttggtt cgcgttctgg
     8161 ctggcgtggg cagaacttcc cggtgctcta taaccaactg ccggatcgta atgccattgt
     8221 ctttgcaacc aacgacaaac ggccggactt cctgcgcgat catccggcgg taaaagcccc
     8281 tgtgattgag atgattaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg
     8341 tcgtgacgac aaagacctgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt
     8401 ccgtggtgaa agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta agccgtacga
     8461 tgcgccgaac tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga aaacctatga
     8521 agaacagtta caatccagcg gtcttgagcc agcagcgatt aacgtttcgc taaacctgcc
     8581 gccggatctc tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac
     8641 catgccgccg gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca
     8701 atccttcaat ctgagcagca aacaggaggc gaaccgcctg ctgctgcgga ttccggtatt
     8761 acaaggtttg ctggatggca aaacagatgt ctctattccg gcgctgaaac tgggcgcgac
     8821 caaccagctg cgcttcgact ttgagtatat gaacccgatg ccgggcggtt cggtggataa
     8881 ctgtattacc ttccagccgg tgcagaatca tgtggtgatt ggtgacgact ccaccatcga
     8941 cttctcgaag tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg
     9001 cttcccattc agccggatgg cggatctgtc gcaaaccatc accgtgatgc cgaaagcgcc
     9061 taacgaagca cagatggaaa cgttgctgaa tactgttggt tttatcggcg cacagacggg
     9121 cttcccggcg attaatctga cggtgaccga tgatggcagc accattcagg gcaaagatgc
     9181 cgacatcatg atcatcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct
     9241 attggtgcag gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc cgttccccgg
     9301 cattgtgccg gacgagagcg atcgcgcggc agaaacccgg tcaacgctga cctcttccgg
     9361 tgcgatggcg gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc
     9421 gctgttggca gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg
     9481 caaacgcgcc accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct
     9541 acgtgttggc gacgtttatt acgtaggtca tctgccgtgg ttcgagcgcg tgtggtatgc
     9601 gctggcaaac catccgattc tgctggcggt gctggcggct atcagtgtga tattgctggc
     9661 atgggtactg tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc cggataacga
     9721 gtaattgaag atgaatgtgt tgcgtagtgg aatcgtgacg atgctgctgc tggctgcctt
     9781 tagtgttcag gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag
     9841 tcaggaaggg cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca
     9901 aagttacggc atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatattct
     9961 cgactggacg cagaacaatc tcgctcaggg ttctttaaaa gaacgtttgc ccgcctggct
    10021 gtggggcaag aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg
    10081 tgatatctgg atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta
    10141 taccgacatc ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc
    10201 tgggctgggt tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg
    10261 ttttaacccc agctacctgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc
    10321 gtggaccacg ctgcgcgaaa ccaatcaacg tttattgctg gaaaccgccc cgaaaggttt
    10381 ttcgccagac tgggtgcgct atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac
    10441 attgatcagc agctacgacg ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag
    10501 cgatccgcaa aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa
    10561 aaacggttat ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc
    10621 ggtcggtttt tctgccgcca tgctgccctt tttacaaaac cgcgatgcgc aggccgttca
    10681 gcgccagcgc gtggccgata actttcccgg cagcgatgcc tattacaact atgtgctgac
    10741 cctgtttgga caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt
    10801 acctgactgg ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt
    10861 ctggccgtca tgccgatggt cgaggcagca ccaaccgctc agcaacagtt gctggagcaa
    10921 gttcggttag gcgaagcgac ccatcgtgaa gatctggtgc aacagtcgtt atatcggctg
    10981 gaacttattg atccgaataa cccggacgtc gttgccgccc gtttccgttc tttgttacgt
    11041 cagggcgata ttgatggcgc gcaaaaacag ctcgatcggc tgtcgcagtt agcgccgagt
    11101 tcaaatgcgt ataaatcgtc gcggactacg atgctacttt ccacgccgga tggtcgtcag
    11161 gcactgcaac aggcacgatt gcaggcgacg accggtcatg cagaagaagc tgtggcgagt
    11221 tacaacaaac tgttcaacgg tgcgccgccg gaaggtgaca ttgctgtcga gtactggagt
    11281 acggtggcga aaattccggc tcgccgtggc gaagcgatta atcagttaaa acgcatcaat
    11341 gcggatgcac cgggcaatac gggcctgcaa aacaatctgg cgctattgct gtttagtagc
    11401 gatcgccgtg acgaaggttt tgccgtcctg gaacagatgg caaaatcgaa cgccgggcgc
    11461 gaaggggcct ctaaaatctg gtacgggcag attaaagaca tgcccgtcag tgatgccagt
    11521 gtgtcggcgc tgaaaaaata tctctcgatc tttagtgatg gcgatagcgt ggcggctgcg
    11581 caatcgcaac tggcagaaca gcaaaaacag ctggccgatc ctgctttccg cgctcgtgcg
    11641 caaggtttag cggcggtgga ctctggtatg gcgggtaaag ccattcccga actacaacag
    11701 gcggtgcggg cgaacccgaa agacagtgaa gctctggggg cgctgggcca ggcgtattct
    11761 cagaaaggcg atcgcgccaa tgcagtggcg aatctggaaa aagccctcgc actggacccg
    11821 cacagcagca acaacgacaa atggaacagt ctgctgaaag taaaccgcta ctggctggcg
    11881 atccagcagg gcgatgctgc gctgaaagcc aataatcctg accgggcaga acgcctgttc
    11941 cagcaggcgc gtaatgtcga taacaccgac agttatgcag tgctggggct gggcgatgtg
    12001 gcgatggcgc gaaaagatta tcccgccgcc gaacgttatt atcagcagac cttgcgtatg
    12061 gacagcggca acactaacgc cgtgcgcggg ctggcaaata tttaccgcca gcaatcgcca
    12121 gaaaaagctg aagcgtttat cgcctcgctc tctgccagtc agcggcgtag cattgatgat
    12181 atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg cagaggcact ggaaaaccag
    12241 ggcaaatggg cgcaggcggc agcacttcag cggcaacgac tggcgctgga ccccggcagc
    12301 gtatggatta cttaccgact ttcgcaggat ctctggcagg ccggacaacg cagccaggcc
    12361 gatacgttaa tgcgcaatct ggcgcagcag aagccgaacg acccggagca ggtttacgct
    12421 tacgggctgt acctctctgg tcatgaccag gacagagcgg cgctggcgca tatcaacagc
    12481 ctgccgcgcg cgcagtggaa cagcaatatt caggagctgg ttaatcgact gcaaagcgat
    12541 caggtgctgg aaaccgctaa tcgcctgcga gaaagcggca aagaagctga agcggaagcg
    12601 atgctgcgtc agcaaccacc ttccacgcgt attgacctca cgctggctga ctgggcgcag
    12661 cagcgacgtg attacaccgc cgcccgcgct gcatatcaga atgtcctgac gctggagcca
    12721 actaacgccg atgccattct ggggctgacg gaagtggata ttgctgccgg tgacacagcg
    12781 gcggcacgta gccagctggc gaaactgccc gctaccgata acgcctcgct gaacacacag
    12841 cggcgcgtgg cgctggcgca ggcgcagctt ggagataccg cagcggcgca gcagacgttt
    12901 aataagttga tcccgcaggc aaaatctcag ccaccgtcga tggaaagcgc gatggtgctg
    12961 cgcgacggtg cgaagtttga agcgcaggcg ggcgatccaa agcaggcgct ggaaacctac
    13021 aaagacgcca tggtcgcatc cggtgtgact acgacgcgtc cgcaggataa cgacacattc
    13081 acccgactga cccgtaacga cgagaaagat gactggctga aacgcggcgt gcgcagcgat
    13141 gcggcggacc tctatcgcca gcaggatctt aacgtcaccc tcgagcacga ttactggggt
    13201 tcgagcggca ccggtggtta ctccgatctg aaagcgcaca ctaccatgtt gcaggtggat
    13261 gcgccgtatt ctgacgggcg gatgttcttt cgcagtgatt tcgtcaatat gaacgtcggc
    13321 agtttctcca ctaatgccga tggcaaatgg gatgacaact ggggcacctg tacattacag
    13381 gactgtagcg gcaaccgcag ccagtcggat tccggtgcca gcgtggcggt cggctggcga
    13441 aatgacgtct ggagctggga tatcggtacc acgccgatgg gcttcaacgt ggtggatgtg
    13501 gtcggcggca tcagttacag cgatgatatc gggccgctgg gttacaccgt taacgcccac
    13561 cgtcggccca tctccagttc tttgctggcc tttggtgggc aaaaagactc cccgagcaat
    13621 accgggaaaa aatggggtgg cgtacgtgcc gacggtgtgg ggctaagtct gagctacgat
    13681 aaaggtgaag caaacggcgt ctgggcatcg cttagtggcg accagttaac cggtaaaaat
    13741 gtcgaagata actggcgcgt gcgctggatg acgggctatt actataaggt cattaaccag
    13801 aacaatcgcc gcgtcacaat cggcctgaac aacatgatct ggcattacga caaagatctg
    13861 agtggctact cactcggtca gggcggttac tacagtccgc aggaatacct gtcgtttgcc
    13921 ataccggtga tgtggcggga gcgcacggaa aactggtcgt gggagctggg ggcgtctggc
    13981 tcgtggtcgc attcacgcac caaaaccatg ccgcgttatc cgctgatgaa tctgatcccg
    14041 accgactggc aggaagaagc tgcgcggcaa tccaacgatg gcggcagcag tcagggcttc
    14101 ggctacacgg cgcgggcatt acttgaacga cgtgttactt ccaactggtt tgttggcacg
    14161 gcaattgata tccagcaggc gaaagattac gcacccagcc atttcctgct ctacgtacgt
    14221 tattccgccg ccggatggca gggtgacatg gatttaccgc cgcagccgct gataccttac
    14281 gccgactggt aagttttcag atagcgcctc tcttaatgcc gctgcgatcg ggtatactcg
    14341 ggcggcaatc tgggatttcc ggggggagac aatttgcgcg taagtcgctc gttaacaatc
    14401 aagcagatgg caatggtggc agccgttgtc ctggtgttcg tttttatttt ttgcaccgtt
    14461 ttgctgttcc atctggtcca acagaatcgc tataacacgg ctacgcaact ggaaagcatt
    14521 gctcgctctg tccgcgaacc cttatcttca gctattttga aaggcgatat tcccgaagcg
    14581 gaagctattc ttgccagcat taaaccggca ggcgtggtca gccgtgccga tgtagtgctg
    14641 cctaaccagt tccaggcgct gcgtaaaagt tttattccag agcgcccggt gccggtaatg
    14701 gttactcgcc tgtttgagct accggttcaa atctcgctgg gcgtttactc gctcgaacgt
    14761 ccggcaaacc cgcagccaat tgcctatctg gtactacagg cggattcctt ccgtatgtat
    14821 aagttcgtga tgagcaccct ctcaatgtta gtgaccattt acttactttt gtcgcttatc
    14881 ctgaccgtcg ccatcagctg gtgcattaac cgcctgattt tgcatccgtt acgcaatatt
    14941 gctcgcgaac ttaacgccat cccagcccag gagcttgttg gtcaccaact ggcattaccg
    15001 cgtctgcatc aggacgatga aatcggtatg ttggtgcgca gttacaacct caaccagcaa
    15061 ttgctgcagc gccattatga agaacagaac gaaaatgcga tgcgcttccc ggtgtcggat
    15121 ttgccgaaca aagccttgct gatggagatg ctggagcagg ttgtcgcgcg taaacaaacc
    15181 accgcgctga tgatcatcac ctgtgaaacc ctgcgtgata ctgcgggcgt gctgaaagag
    15241 gcgcaacgag aaattctgct gctgacgctg gtggaaaaac tcaaatcggt actgtcgcca
    15301 cgtatgatcc tcgcgcagat tagcggttat gactttgctg tcattgccaa cggtgtacag
    15361 gaaccgtggc acgcaatcac cttaggtcag caagtgctca ctatcatgag cgagcgcctg
    15421 ccgattgaac gtattcaact ccgtccgcac tgtagcattg gcgtggcgat gttctacggc
    15481 gatctcaccg ccgaacagct ttacagtcgc gctatttctg cggcatttac cgctcgccat
    15541 aaaggcaaga atcagattca gttctttgat ccgcagcaga tggaagccgc ccagaagcgg
    15601 ttgacggaag agagcgatat ccttaatgca ctggaaaatc atcagtttgc tatttggtta
    15661 cagccacagg tcgagatgac cagcggaaaa ctggtcagtg cggaagtgtt actgcgtatc
    15721 cagcaaccgg atggcagttg ggacctgccg gatggcttaa tcgatcgcat tgagtgctgt
    15781 gggctgatgg ttaccgtcgg tcactgggtg ctggaagagt cctgtcgatt gcttgcagcc
    15841 tggcaagagc gcggcattat gctgcccttg tcggtaaacc tctctgcgct gcaactgatg
    15901 cacccaaata tggtggcgga tatgctggaa ctgttaaccc gctatcgcat tcagccggga
    15961 acactgattc tggaagtgac agaaagccga cgtattgacg accctcatgc tgcggtggca
    16021 atcctccgtc cgctgcgcaa tgccggagtt cgggtggcgc tggatgattt cggcatgggc
    16081 tacgcagggc tgcgtcagct gcagcatatg aaatcgttgc caatcgacgt actgaaaatc
    16141 gacaaaatgt ttgttgaagg cttgccggaa gatagcagca tgattgctgc aattatcatg
    16201 ctggcgcaga gcctgaactt acaaatgatt gccgaaggcg tggagactga agcacaacgc
    16261 gactggctgg caaaagcggg cgttggtatt gcccagggct tcctttttgc tcgcccactc
    16321 cctattgaaa tcttcgaaga gagttacctg gaagaaaagt agctacccca aactgattac
    16381 aaaactttaa aaagtgctgg tttgtgcgag ccagctcaaa ctttttaacc tttttgtttc
    16441 aattatgatc caggtacatt tctgtgatgt tgtctgggtg ttattttaag gccgcaggta
    16501 ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa acctctctgt
    16561 ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc cttggccatt
    16621 tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt aagctcatta
    16681 agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg ggcatggaaa
    16741 gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa attgtcagta
    16801 ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt gccggaatga
    16861 acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat caggcgaaag
    16921 accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt ggcgcatttg
    16981 ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt gcgctccacc
    17041 gtctgggcag caaaggccaa ctgattttta acgtcatcga aagtttctcg caggtcatct
    17101 tcggcatcat caatatgatc atgcgtctgg cgcctattgg tgcgttcggg gcaatggcgt
    17161 ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg attatctgtt
    17221 tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct aaagcgactg
    17281 gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt gtactgggga
    17341 cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa ctcggctgcc
    17401 gcaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt gatggcacat
    17461 cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt cagatggata
    17521 tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg gcggcagggg
    17581 taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc catttgccgg
    17641 tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct cgtgcgctga
    17701 ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg aaagaactgg
    17761 accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa acgcacgaat
    17821 tatcctctta atctcatcac ttatgcccgc actcccttcc ccgagtgcgg gtgtttgcgc
    17881 ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat ttcacttctt
    17941 ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc atttagactg
    18001 accgacaacg ggcagttttt tattaccagg atagttgatc aggggttcac atgcagggca
    18061 caaaaattcg acttttagcg ggcggtttgc tgatgatggc cactgctggc tatgtgcagg
    18121 cagatgcgct ccagcctgat ccagcatggc aacaggggac gctttccaac ggtttacagt
    18181 ggcaagtgct gaccaccccc cagcgtccca gcgatcgtgt tgaaattcgc ctgctggtta
    18241 ataccggttc gctcgccgaa agtacacaac agagcggtta cagtcacgcc atccctcgta
    18301 ttgcgctaac gcaaagcggt ggccttgacg cagcacaggc gcgttcattg tggcagcagg
    18361 ggatcgaccc taaacgcccg atgccgccgg taattgtctc ttatgacacc acgctgttta
    18421 atctgagttt gcccaataac cgtaacgatt tgctgaaaga agcgctctct tatctggcaa
    18481 atgccactgg caaattgacc atcacaccag aaaccatcaa ccacgcgctg caaagtcagg
    18541 acatggtggc aacctggcct gccgatacta aagagggctg gtggcgctat cgtctgaaag
    18601 ggtcaacctt gttaggtcac gatcctgccg atccgctgaa acaacccgtt gaagcggaaa
    18661 aaattaaaga tttctatcag aaatggtaca ccccggatgc aatgacgcta ctggtggtgg
    18721 gaaacgtgga tgcgcgctcg gttgtcgacc aaataaacaa aacgtttggc gaactgaaag
    18781 gcaaacgtga aacgccagct ccggtgccga cgctttctcc gctgcgtgcg gaagcggtga
    18841 gtattatgac tgacgcggtg cgtcaggacc ggttatctat catgtgggat acgccgtggc
    18901 agccgattcg tgaatcagcc gcactgctgc gctactggcg tgcggacctg gcccgcgagg
    18961 cgctgttctg gcatgttcag caagcgttaa gcgccagtaa cagcaaagac atcggtcttg
    19021 gatttgactg ccgtgtgctg tatctgcgtg cgcagtgtgc catcaacatc gaatcaccaa
    19081 acgacaagct gaacagcaac cttaatctgg tggcgcgtga actggcgaag gttcgcgata
    19141 aaggtctgcc ggaagaagag ttcaatgcgt tagtggcgca aaagaaactg gagctgcaga
    19201 aactgtttgc cgcctatgcg cgggctgata ccgatattct gatgggtcag cggatgcgtt
    19261 cgttgcaaaa tcaggtcgtc gatatcgcgc cggagcagta tcagaaactg cggcaggatt
    19321 tccttaacag cctgacggtg gagatgttaa atcaggatct gcgtcagcag ttgtcgaatg
    19381 atatggcgtt aatactgctg cagccgaaag gcgagccgga atttaacatg aaagcgttgc
    19441 aggcggcctg ggatcaaatc atggccccat cgactgctgc tgccgccacc tctgtcgcca
    19501 cggatgacgt acatcctgaa gtgacggata ttccacctgc acagtaatgc ctgatggtga
    19561 tgagttagca caaccatcag gtatttatgg atgagctgga tagaggggta acactttatc
    19621 cagccttttg catatgctgc gtttacgctg gcatcgcctc acgcgggata atcgcgccgc
    19681 gatactgaat aacggtactt gcggtcaggt gcccgcgttt cgccgcgttt tccgcactgc
    19741 cgcccgtcaa acgtaccgcc agataaccgg cgctgaaaga gtcgccagct gcggtggtat
    19801 cgatcacttt ttctttcggc agtttcaccg ccggaacatc cactaaccct tcgccagcaa
    19861 tggacaccag gcaagaatcc gccccgcgtt tcaccaccac ttctttcacg cccgcgttat
    19921 gggtgcgcgc aatgacgtct tccaccggct gttgacccca cagcgcgtct tcatcgtcca
    19981 gcgtcaggaa ggcgatatcc gtgcattcca gcatttgttg gtacacctgc tgtgtctctt
    20041 ctttgctggc ccacaggcgc ggacgatagt tattgtcgaa aatcactttt ccgccgttgg
    20101 cgcggcattc gcgcagcagg gaaagcagct tttcgcggct ggtcgggctt aagatcgcca
    20161 ggctaatccc gctcaggtag agataatcga aattcgccag ctcttcgcaa atcgccgcag
    20221 actgctcact ctccagccag aatttggcgg cggcttcgtt ccgccagtag tagaacgtac
    20281 gctcgccggt gctgtcggtt tcaatgtagt aaaggcccgg cagacggttt tccatccgtt
    20341 gggtcaggga agtatcaacg ttctcgccgt gccaggcgtc cagcatttgc tggctaaaac
    20401 tgtccgttcc cagcgccgtt acgtaatgaa cggttaatgc cgcaggatcg acctgacggg
    20461 cgatatagac ggaagtgttc agggtatcgc cgccgaaacc gcgcttaacg tccgcgcctt
    20521 tctcggaaag ctcaatcatg cattcgccaa tcacggcaat ctttttggac atagtcgtga
    20581 acctgatctg taaaaaatta gcgttagttt gcgctgtggt ggtttgctgg tcaaccatat
    20641 taaaacagtg ttccattatt tttttgagcc agaacgtatt cctgaaagat tccgttgtgg
    20701 agtgaggaaa tttaccccgg ccaggcggta aagttctgcc cttgcgcgcc gataatcttt
    20761 gtcgagtccg ggcagcatca cttttaaaca caggacatct ttgatgataa ggcaggttat
    20821 ccagcgaata agcaaccctg aagcaagcat cgagagcttg caggaacggc gtttttggtt
    20881 gcagtgtgag cgtgcttaca cctggcagcc gatctatcaa acatgcggac ggttaatggc
    20941 cgtggagcta ttaacggtgg tcacgcatcc cttgaaccct tcgcaacgcc tgccgccgga
    21001 tcgctatttt actgaaatca ccgtcagcca tcggatggag gttgtgaaag agcagattga
    21061 tttgctggcg caaaaagccg acttctttat agagcacggc ctgctggcat cggtcaatat
    21121 tgatggccct acgctcatcg ccctgcgtca gcaaccaaaa atcctgcgcc agattgagcg
    21181 tcttccctgg ctgcgtttcg aactggtgga gcatatccgt ctgccgaaag attcaacctt
    21241 tgcctcgatg tgtgaatttg gcccgctgtg gctggatgat tttggtaccg ggatggcaaa
    21301 tttctctgca ctaagtgaag tgcgttatga ctacatcaaa atcgcgcgag aactgtttgt
    21361 gatgctgcgt cagtcgccgg aaggacgcac actcttttct cagcttttac atctaatgaa
    21421 tcgctattgt cgcggggtga ttgtcgaggg cgtagaaacg ccggaagagt ggcgtgatgt
    21481 tcagaactcg cccgcattcg ccgcacaagg ctggtttctt tcacgcccgg caccgataga
    21541 aacgctgaat acggcggttc tggcgctata agctgcctca ttttccgcct ggctggacta
    21601 tctttaggac tggcacagga agaatatgag gaagcgaacg atgagcaagg caggcaaaat
    21661 aaccgctgcg atttcagtgg ctttcttgtt gttgattgtc gtggcgatca ttttgattgc
    21721 aacttttgac tggaatcgac tcaaaccgac catcaaccag aaagtctctg cggagttgaa
    21781 tcgtccgttc gctatccgtg gcgatctggg cgtggtgtgg gagcggcaaa aacaagaaac
    21841 tggctggcgc agctgggtgc cgtggcccca tgtacacgcg gaagacatca ttcttggcaa
    21901 tccaccggat attcccgaag tcacgatggt gcatttgcca cgcgtagagg caacgctggc
    21961 cccgctggcg ctgctgacca aaacggtctg gctgccgtgg atcaagctcg aaaagcccga
    22021 cgcgcgcctg attcgcctct ccgaaaagaa caataactgg acgtttaatc tcgccaacga
    22081 tgataacaaa gacgcgaatg caaagccgtc ggcatggtcg tttcggctgg ataatattct
    22141 tttcgatcaa gggcggatcg ccattgatga caaagtaagc aaagcggatc tggagatttt
    22201 tgttgatccg ttaggcaagc cgctgccgtt cagcgaagtt actggatcga aaggtaaagc
    22261 ggataaagaa aaggtgggcg attacgtttt tggcctgaag gcgcagggac gttataacgg
    22321 tgaaccgctc acgggtacgg gaaaaatagg cggtatgctg gcgctgcgtg gcgaagggac
    22381 gccgtttccg gtacaggctg atttccgctc tggtaacacc cgtgttgctt ttgatggcgt
    22441 cgtgaatgat ccaatgaaga tgggcggtgt cgatttacgg cttaaatttt ctggcgattc
    22501 actgggtgat ctctatgaac tgacgggcgt tctgctgccc gataccccgc cgtttgaaac
    22561 ggatggtcgg ctggtagcga aaatcgacac tgaaaaatcg tcggtctttg attatcgcgg
    22621 ttttaatggg cgaattggcg atagcgatat ccacggttct ctggtctaca ccaccggaaa
    22681 gccacgacca aaactggaag gtgatgtcga gtcgcggcaa ttgcggctgg cggacctggg
    22741 accgttaatt ggcgttgact ccgggaaagg ggcagaaaag tcgaaacggt ctgaacagaa
    22801 gaagggcgaa aaaagcgttc agcctgcggg caaagtgctg ccttatgacc gcttcgaaac
    22861 cgataaatgg gacgttatgg atgccgatgt tcgcttcaaa gggcggcgca ttgagcatgg
    22921 cagtagcctg ccgattagtg atctttctac tcatatcatc ctcaaaaatg ctgacctgcg
    22981 cctgcaaccg ctgaaatttg gcatggcggg cggcagcatt gcggcgaata ttcatctgga
    23041 aggcgataaa aagccgatgc aggggcgggc agatattcag gctcgtcgac tgaaactgaa
    23101 agaactgatg cccgatgtgg aactgatgca gaagacgctg ggggaaatga acggtgacgc
    23161 ggaactacgc ggtagcggta actcggtggc ggcactttta ggcaacagta acggcaacct
    23221 gaaactgttg atgaatgacg ggctggtgag ccgcaacctg atggagattg ttgggctgaa
    23281 tgtcggcaac tacattgtcg gtgcgatatt tggtgatgat gaggtgcggg tgaactgcgc
    23341 ggcggcgaat ctggatattg ccaacggcgt ggcgcgcccg cagatctttg ctttcgatac
    23401 tgagaacgcg ttgattaacg ttaccggcac ggcaagtttt gcttccgaac agctggattt
    23461 gactattgat ccggagagta aaggaattcg gattatcaca ctgcgttcgc cgctgtatgt
    23521 gcgtgggacg tttaaaaatc cgcaggctgg ggtgaaagcc gggccgttga ttgcccgtgg
    23581 tgctgttgct gcggcactgg caacgctggt aacaccggcg gcggcgttac tggcactgat
    23641 ctcaccttcc gaaggggagg ctaatcagtg tcggacgata ttgtcgcaga tgaagaagtg
    23701 atgaggttgg attgtgtttg ctggtgagtt cgggagcagc gtgttcccct caccctaacc
    23761 ctctccccaa aggggcgagg ggactgtttt gtgcgtgttg aaacaacgag taccgctttt
    23821 ccgttcactt tctgggggag ggctgttatg ttcgcgatat aacatctcgc tccgcttttc
    23881 tccctctccc tttcagggag agggctgggg tgagggtggc tgcccacacg atacttacaa
    23941 cgactgatgt cgcgtctcat gggtcagcag cagggcgatt aacgtcaagc cagccatcgc
    24001 cgccagatat aaccccaccg cacctaaccc gtagttagcc tgcaaccagg ctgcgatata
    24061 tggtgcaacg gaagccccga gaatcgacgc tacgttgtaa gagaacgatg ctccggtgta
    24121 acgcacttct gtcgggaaca gctctggtaa cagcgcgccc atcggcccga aggtcagtcc
    24181 catcagactt aaccccagca gcaggaaggc aaaaaccaga atcgggttgc cagaaccgag
    24241 cagcgggtta aaggcgaaca gcgcgaacag gatgatcagc gtggtgatga ttaccatgct
    24301 tttacggcga ccaaaggcat cagccagtaa tccggcgact ggcaccatca cgccaaaacc
    24361 aataactgcc atcatcagca tccacaacac ttcgttacgc ggcaggccaa gcccaactgg
    24421 cgcggcggcg gtactgaagg tcatagagta gaccgtcatg atgtaaaaca gcgtataggt
    24481 tgccagcata atgaacgtac ccagtacggt tacgcgaaca tgtttggtca gcagcgtacc
    24541 cagcgggatc ttcacctgtt ttttcgcttt agcgactttc tcaaacaccg gcgactcatg
    24601 cagcgacacg cgaacataca ggccgataat gaccagcacc gccgagaaga taaatggcac
    24661 acgccagccc cagctcataa actgctcgtc ggtcagtagc caggaaagca gcaagaaagt
    24721 gccgttggca aagaagaagc caatcggtgc gcccagctgt gggaaggagc catacagtgc
    24781 acgtttgcgc ggtggggcgt tttcagtcgc cagcagcgcc gcgccgcccc attcaccgcc
    24841 taagcccaga ccctgaccaa atcgagccag cgccagcagc agcggggcga aaataccaat
    24901 cgtggcatag cccggcagca gaccaatcac cacggtcgaa atccccatcg ttagcaacga
    24961 ggcgaccagc gtcgctttac gcccaacgcg atcgccaaaa tgaccaaaaa cggcagagcc
    25021 aatggggcgc gcgacgaagg cgatggcgaa ggtggcgagc gactgtagcg ttgctgccgt
    25081 tggatcgccc tgcgggaaga agatatgcgg aaacacaata acggccgcag tggcgtaaat
    25141 gtaaaagtcg aagaactcaa tggccgtgcc aatgagagag gcgacaagga ctttattacg
    25201 cgagttgatc ggcgtgtatt cttgctcgtg gtcgagtgtt gtggctgttg cttgcataat
    25261 cttttcttat tttttggcta acgaatagcc atattacgca cagcaaaagt gatatttcaa
    25321 tgtgtaacaa atcgcggaaa tggtcaaaaa ccaggcaaaa aggggataat gtttaggcca
    25381 gtgatttttc ttctatgaaa tgcttcacat aattttataa atagtggata aaactggttt
    25441 caggttaaat aaaagttacc aactggattt atatgctggg gttatgagtt ggaatgaaat
    25501 tgtttgctcc caaatagatg gctgaagagg ctctggggaa gagtttcaag aggttatgtg
    25561 cgcattatag ccggatgcga tgctggcgca tcttatccgg cctacgggga gccaggccga
    25621 ataaaatcgt atgccggata aggcgtttac gccgcatccg gcctatttta aggctgcgtt
    25681 ttccctggca ttcgcgggtc gtctttatat tcggcggtgg caatccacgc cgcgcaaaac
    25741 agcgtcaaac gggcgaagaa gtagaaaaat gccatcagcc ccagcacgga accaaatgct
    25801 gcgccagagg gggatttcat cagcgatggc aaggtgtagg tcatcacgat tttaatcact
    25861 tcaaaaccaa tagcagccag aaatgttccg cgaatcaggg cttttttgcg cggacggtgg
    25921 cgcggcaacc gccagaagat ccagaagaaa agcagatagt tggcgaagat ggaaatcgcc
    25981 aggccaatca accgccacgt cggtttcagc cactcaatgc tgttcaggtg cagggcgcta
    26041 ataatcattt gctgcgccga accggcaacc gaggtgatcg acagcgtcac aatcagcgca
    26101 attaacaaac caatcagtga aataaaatca cgcagatatt ttacccagaa cttctcctga
    26161 tcttgcggcg agcgttccca gacatcgcgc gactgggcac gaatcgcttc acgcaggtta
    26221 cccatccagt tgatgccgga ataaagtgcc accgccaggc cgacaagccc tacagtcgta
    26281 cgctgctgaa cggcggtgtt gatggtgttt ttcaacgtgg cggctagcgt cggatcgctg
    26341 atgttttgca gaattttgtc gaagatatcc tgtagcaaca tcggatggga ggccagcaca
    26401 aagccccctg cggcaaacga caccatcaaa atcgggatca tcgacaaaaa tgagaaatag
    26461 gtgatagccg caccaaactg gttacccagc cgatcgttaa agcgttctgt cgcacgaatc
    26521 aggtgcgcaa taaccggttg gcgttggact ttttcggcag tggtggtgac ggtttccagc
    26581 gcctggctaa ctttgccgcc tttctcgcta ttatccaact gtttaatcgg ctcgtgctcc
    26641 agatcctggg tgggacgttt gatctcgttt tcctgcgtca tccgctcttt tgtccttctg
    26701 tcgtggtcat tcttaaaaag tatagtcggt cagtccaggt gctctttcat tacgccagcc
    26761 agccactgca taaacaggtt tacacgccgg gaaagctccc gacgctgcgg gtaaaccagc
    26821 gaaagggaga gcggctcggc acggtagcct ggcaacactt caataagccg cccggcacgc
    26881 agggcttcgc gcacggcaat gcgcgggatc tgaataatcc ccagaccggc aagaccggcg
    26941 gcgagatagt tttcgctact gtttaccgtc aacatgccgc cagacttaaa ccactggacg
    27001 ccattaacgc tggcaacctc aaaacctaac ggatgtacac ccaggtgcgg tgtataacgc
    27061 actattgcgt gtgaagtcag atcatcgggg ctttgcggat aaccaaagcg cgtcagatag
    27121 tgcggactgg cacagttgac catggtcagt ttgccgaggg gacgggcgat aacgccatct
    27181 tccggtaacg cgccagtgcg tatcacgcaa tcaaaaccat catgaagaat atctaccgga
    27241 cggtcatggc tactcagttc cagctcaatt cccggatgca gatagagaaa ttccgacagg
    27301 cgcggcagta acaggctttt cgcgattcct ggcgggatgt cgatgcgtaa tttaccgctg
    27361 atactggtgg catcctgttg aaacagaccg tccagttcgc tgagattact caacacatct
    27421 tttgctcgtt gataataggt catgccttct ggcgtgagtt tgacccgtcg cgtggtgcgc
    27481 tggagaagct gggtgccaag ttgatgttcc agtgcctgta tctggcgcga aacacttcct
    27541 tttggcaaag caaagaaatc cgccgcgcgg gaaaaacttt ccagctccgc gactttgatg
    27601 aacaactgca ttgcgtgaat tttatccatt tctttgaccg attgttgttc acagcgaaac
    27661 agtaacgcgc atatcgcatt gtttattgtt ttttctaaca tctaatgccg ggtttcacaa
    27721 tgttgtcatg acgaatgtgg cgatttctca gcaggaaaag ggcggaatag ctttctgaga
    27781 aaggtgaatc cccctaaaca gagcgtattt ttcagcgatt ccctttaaag tgaatagagg
    27841 acgtgttggc tatttgtgct tatttttgtc tcattgccgt ttgttatttt tgcttgtggt
    27901 cgcggagttt catttttaat tgcatgcgta ctaaccacca atataaggaa atcctcaaaa
    27961 ttgttttaaa tagtgccctc gacatgtaat ttcaggcgtt tagctgtaac tatttatatc
    28021 ggtaactatt ttctacgcgt catctctgta gaatggccac cgccaaatgg cgaattctta
    28081 ttttttctcg cgggtaatac ccttccacta cttaagtctt tttaacgtat tcaggtggag
    28141 attcagttat gcaaatagtc atgtttgaca ggcagtcaat atttattcat ggaatgaaaa
    28201 tcagtttaca gcagcgtatt ccaggagtga gtattcaggg ggccagtcag gcagacgagt
    28261 tatggcaaaa gctggaaagt taccctgaag ccttagttat gctcgatggc gatcaggatg
    28321 gtgagttttg ctactggttg ctgcaaaaaa ccgtggtgca gtttcctgag gttaaggtgt
    28381 taatcacggc gacggattgc aataaacggt ggttacagga agttatccat tttaatgtgc
    28441 tggccattgt gcctcgtgat tcaaccgtcg agacatttgc gttggcggta aatagtgcgg
    28501 cgatggggat gatgtttctg cctggagact ggcggacgac gccggaaaag gacattaaag
    28561 atctcaaatc gttaagcgcc cgccagcggg agattttaac catgttagcg gcgggcgaat
    28621 caaataagga gatcggcaga gcgctgaata tcagtaccgg aacggtaaaa gcgcatctgg
    28681 aatccctgta tcgccgtctg gaagtgaaaa atcgcaccca ggcggcaatg atgttaaata
    28741 tctcctcctg aagaggataa ttttacagcc agcaggaagt tagcggctgg ctgtaaaaat
    28801 attatggttc gccgtacaaa ccaattaaac ggcgtaccac accgttagtc cagccaaacc
    28861 catcctgcaa cggatactcg ccaccgccgc cttcgcgggg aacaccatcg gcaatatggt
    28921 atttctcgat cattttgtgc tgttccagat agaactgatt caccgttttc agccagctgc
    28981 gcgcgatttc atcacccaga aggtcatcgc catacatttt aaatccctga attgccatcc
    29041 attgtaacgg tgcccagcca ttgggtttat cccactgttc accggtttcg tactcgcttg
    29101 ccagaatccc gccaggtgtc agtaaccggc tgcgtacggc gtttgccaga cgatcggcct
    29161 gttcatggtt cgccatgccg acatagagcg gcacaatggc ggcagcggaa aacagcgcca
    29221 gttgttcgcg tcgccagtcg taatcgcggt agatgccgtt ttcatcatcc cagaggtaac
    29281 ggtttaccgc atcgcgacgg gcactggcct tctggcggaa cagcgcttct gtctctttct
    29341 cgcctttcag cgccgagatg ttggcgatgg cgctctccag tttaaacagg aaggcattca
    29401 gatcgatggg gatgaactgg gtggtacgaa tgctcgccag acgaccagta tcacgcagcc
    29461 aacgggaaga gtaatcccaa cctgaggccg ctcccgcgcg taaatcgcgg tacacctcgt
    29521 tgggcgggcg accagaatgt ttcgcggttt caacgtcctc aagccaggat tcgtcacgcg
    29581 gcgtgtcgcg atcgtcccaa taacggttga gcagcgatcc gtccggcatc cgcacaacat
    29641 ggcgataggc ctgattaggg atcaacgatt ctgcaccgtc catccagaag gcatattcca
    29701 ttttaaggtg gtcgagatag cggcgcgcac cgcgtacacc atcttcttca aacaactcca
    29761 ccatcagcgc aaaaaccggt ggttgcgatc ggctcaaata ataggtgcgg ttgccgttgg
    29821 ggatgtgacc atagttttcg atcatccagg cgaagttatc ggccatgcat ttcagcaaat
    29881 cttcccgacc actttccgcc agccccagca tggtgaaata ggaatcccaa tagtaggttt
    29941 cgctaaaacg gccgcccggg acaatatatg actgcggtag cgccagcaga gaagaccacg
    30001 gaatgtgatc ctgtggttcg cgggttagca ccggccacag ctggtcgata tgctctttca
    30061 gggaattttg cgggtccgat acatactcgc tggagtagac ctccggcagc cagaagtgat
    30121 tttcaacaaa cttgcgcaag tcaaaatcac gatgacggcg cactttacgg tagcggatta
    30181 aaatatccag cgggtccatt ttgggtgcgc agtcgggaaa ggttttactg tcggggaaaa
    30241 ttttcgccga ctgaacatgc tcgaacagtt cgagatagcg atcggcaggc gtcagcgcat
    30301 cagaggcggg cagcccttca atcatctcgg gttccggttc tgcctcgatc atctcatcca
    30361 gttttaattc atacgggtcc agttcatagc agagatcgac ctcgatcatc agttcgtctg
    30421 gattagggtt ttgaattttc tgattgagca taaggatcca acctccgaaa ttcgtcgcta
    30481 attatcgggc ttttctttaa gggtagtcat tcgctccgcc tggtgggcga caaagcgtgc
    30541 ggtggatcac gtttattaac ttgtcaaaaa atataacgca ctaatatcaa tggaatttat
    30601 aaaaatacgg atttcgtgtt gtttttcatt ttcagaacat tcaatcctgc tctcgccgca
    30661 atcatgatat gccattcctg atctgggtaa taggcacagg ctatcttatt gatagtttat
    30721 attcatgtaa ttgattgatt cctatcaaaa agcgtatctc cctgtttttt atgatggctt
    30781 tgtctcgcag agacgtttgc agcattctgg aaaattttct gggtcacaat aactcaccac
    30841 cgtgagggaa gaataatgaa aatggtctca cgtattaccg cgatcggcct ggctggcgtc
    30901 gcgatttgct atttagggtt atctggttat gtgtggtacc acgataataa acgcagtaaa
    30961 caggccgatg ttcaggcatc tgctgtcagt gaaaataata aggttttagg ctttctccgc
    31021 gaaaaaggat gcgactattg ccacacgcct tcggcagaat tacccgccta ttattatatt
    31081 cctggcgcga aacagttgat ggattacgac attaagcttg gatataaatc ttttaacctc
    31141 gaggccgtgc gtgcggcact gctggctgat aaacccgttt cgcaaagcga tctgaataag
    31201 attgaatggg tgatgcagta tgaaactatg ccaccaacgc gttataccgc gctacactgg
    31261 gcgggtaagg tgagtgatga agagcgggcg gaaatactgg cctggattgc aaaacagcgc
    31321 gcggaatatt acgccagcaa tgatactgct ccggagcatc gcaatgaacc ggtgcagccc
    31381 atcccgcaaa aactgcctac cgatgcgcaa aaagtggcgt tgggctttgc gctgtatcac
    31441 gatccccgtt tatcggctga tagcaccatt tcatgcgctc attgccatgc gttgaatgcg
    31501 gggggcgtcg atggcagaaa aacatcgatt ggtgttggtg gcgcagttgg gccgattaac
    31561 gcgccgacgg tatttaactc agtatttaac gttgagcagt tctgggatgg tcgtgcggca
    31621 acattgcagg atcaggcagg tggaccgccg ttgaacccga ttgaaatggc gtcgaaatcc
    31681 tgggacgaaa ttattgctaa gctggaaaaa gatccgcagc ttaaagcgca gttcctcgaa
    31741 gtttatccgc aaggtttcag tggcgaaaat attactgatg ccattgctga atttgagaaa
    31801 acattaatta cgccggattc cccatttgat aaatggttgc gtggagatga aaatgcgctg
    31861 acggcgcaac agaaaaaagg ctatcaatta tttaaagata ataaatgtgc aacttgtcat
    31921 ggtggtatta ttctcggcgg acgttccttt gaaccgttgg ggctgaaaaa agactttaac
    31981 tttggggaaa ttacggcggc ggatattggt cgtatgaatg tgactaaaga agagcgtgat
    32041 aaattgcgtc agaaagtacc cggtttacgt aacgttgctt taacggcacc gtacttccat
    32101 cgcggtgacg tgccgacgct ggacggggcg gtgaaactga tgctgcgcta tcaggtaggc
    32161 aaagagctgc cgcaggagga tgtggatgat atcgtagctt tcctgcacag tctgaacggg
    32221 gtgtacacgc cgtatatgca ggataaacaa taattaattt gatcgcccga acaggaatgt
    32281 ttgggcgatt tttattacga taataaagtc tgtttttaat attatcatgt taaatgttta
    32341 tattataaaa agtcgttttt ctgcttagga ttttgttatt taaattaagc ctgtaatgcc
    32401 ttgcttccat tgcggataaa tcctactttt ttattgcctt caaataaatt taaggagttc
    32461 gaaatggacc agaagctgtt aacggatttc cgctcagaac tactcgattc acgttttggc
    32521 gcaaaggcca tttctactat cgcggagtca aaacgatttc cgctgcacga aatgcgcgat
    32581 gatgtcgcat tccagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
    32641 ctggccactt tctgccagac ctgggacgac gaaaacgtcc ataaattgat ggatttgtcg
    32701 atcaa
//
LOCUS       DAZKGS010000036        32630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKGS010000036 DAZKGS010000000
VERSION     DAZKGS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..177
                     /locus_tag="ACHOQY_001696"
     CDS             <1..177
                     /locus_tag="ACHOQY_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9677820.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHOQY_001697"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHOQY_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHOQY_001698"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHOQY_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9677821.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHOQY_001699"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHOQY_001699"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9677822.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHOQY_001700"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHOQY_001700"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9677823.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHOQY_001701"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHOQY_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHOQY_001702"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHOQY_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..5368
                     /locus_tag="ACHOQY_001703"
     CDS             4301..5368
                     /locus_tag="ACHOQY_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677824.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHOQY_001704"
     CDS             complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHOQY_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9677825.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHOQY_001705"
     CDS             complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHOQY_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9677826.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHOQY_001706"
     CDS             complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHOQY_001706"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9677827.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHOQY_001707"
     CDS             8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHOQY_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9677828.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHOQY_001708"
     CDS             complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHOQY_001708"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9677829.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHOQY_001709"
     CDS             11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHOQY_001709"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9677830.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHOQY_001710"
     CDS             complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHOQY_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9677831.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHOQY_001711"
     CDS             12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHOQY_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9677832.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHOQY_001712"
     CDS             complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHOQY_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9677833.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHOQY_001713"
     CDS             complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHOQY_001713"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9677834.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15439..17172
                     /gene="tamA"
                     /locus_tag="ACHOQY_001714"
     CDS             15439..17172
                     /gene="tamA"
                     /locus_tag="ACHOQY_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9677835.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17169..20948
                     /gene="tamB"
                     /locus_tag="ACHOQY_001715"
     CDS             17169..20948
                     /gene="tamB"
                     /locus_tag="ACHOQY_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9677836.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGSVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHOQY_001716"
     CDS             20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHOQY_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9677837.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21504..21755
                     /gene="chpS"
                     /locus_tag="ACHOQY_001717"
     CDS             21504..21755
                     /gene="chpS"
                     /locus_tag="ACHOQY_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP9677838.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21749..22099
                     /gene="chpB"
                     /locus_tag="ACHOQY_001718"
     CDS             21749..22099
                     /gene="chpB"
                     /locus_tag="ACHOQY_001718"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP9677839.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHOQY_001719"
     CDS             complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHOQY_001719"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9677840.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHOQY_001720"
     CDS             23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHOQY_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9677841.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHOQY_001721"
     CDS             24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHOQY_001721"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9677842.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLLFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHOQY_001722"
     CDS             25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHOQY_001722"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9677843.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHOQY_001723"
     CDS             26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHOQY_001723"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9677844.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHOQY_001724"
     CDS             complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHOQY_001724"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9677845.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28842..30215
                     /gene="mpl"
                     /locus_tag="ACHOQY_001725"
     CDS             28842..30215
                     /gene="mpl"
                     /locus_tag="ACHOQY_001725"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9677846.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAYI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHOQY_001726"
     CDS             complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHOQY_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9677847.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHOQY_001727"
     CDS             31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHOQY_001727"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9677848.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32551..>32630
                     /locus_tag="ACHOQY_001728"
     CDS             32551..>32630
                     /locus_tag="ACHOQY_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9677849.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         7728 a         8134 c         8910 g         7858 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg acactggagc agggagagca gatgccagaa tggacggaca ggaaaatgga
     5281 gggggagtat aaaagaggcg agaaattcca tgtgctgtgg tcgctgatga accgccatga
     5341 tggcgggtcg gtgtgggtgg aagcgtagcg gtagctggat gtacacgtaa aaaccaggga
     5401 atgggtgatt ttattctggc ttatacagga gcgatgaata agtaattata ttaatgaaaa
     5461 aaataagtta gtaaccgttg aaggaactta ttattggctg aataatgaat tttcccagtt
     5521 cagtagatac aaaagattgt cccggagtgg tgaataactc aaagcatttg aaaacgcacc
     5581 tgattgtgta aaccaggtgc gttgatcgaa caaattactc acccgccagc gcgcgtggga
     5641 acagtacatt gttttccagg ctgatgtgat ccatcaggtc atcaatcagt tcattaatac
     5701 cgttatacat cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg
     5761 tgtgtttaat cacttccagc agttcgcccg cttcatcgtg ctcgctttcc atcacgctga
     5821 ttggtcccat tgcctggctg cccatgcctt gtttgatcat cgggaagagg atctgctctt
     5881 ctttcatcat gtggctggaa agctcttcat gcagcatagt caggtatttt gtcagccctt
     5941 ttggcacgct cggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct
     6001 ccggcagctg ctcgcggtga cgatcgtggt agcgcacgat gatatggtca atgatttctg
     6061 ccagcggggc gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcag
     6121 cttcaatgac ctcaacatcc agttctttac gtgccgccgc gcgcgccagc gtctgcttac
     6181 cgccacagca gtaatccata tcatatttac gaaacagagc cgaagcgcga gggatagaga
     6241 gcgccagttc acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa
     6301 gataagatgt attttaaatg catctttaag gcaaaagcta taacccttac gtagcagagg
     6361 cttatttaac tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc
     6421 tggttttgct ctgaccgcaa tgaccacgcc aatcactaac agcgcgatac cgctcagcgt
     6481 cattagcggc ggcatttgct ggcggagtaa aaaggtgtac agcaaacctg ccagcgtttc
     6541 gaaaacaatc agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca
     6601 gagtgcgcca acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag
     6661 cggacggggg ccaaaaggta aggagaagtc cggcgtttgt atattcagcc agtaacaggc
     6721 gacgagatag ccgatgagag aaaccggcag cgtgaccagc gcctgcgccg tcgcccacat
     6781 catcggatgt ttgtcgggat tttcccgcag ccagcgggcg ttgcgcaggg cataccatgc
     6841 ccagcagacc acggaaacta acgctagcac gatgccagag gtataacgtg cccagtcaaa
     6901 atcggggagt ccgtggttta actcagcaat attcacactc gccaggccga tgccaataca
     6961 aatcagtgcc ggggcgagtt ttccccacgc gagtttgccg tcgcgctggc tataaagcag
     7021 attggcaaag acaggaatga ccaccggcag ggtgccgata atcatcgtgg aaacaggcgc
     7081 gccagtacgt tgaatggcac tggcaaggca gaagtaataa atgaggttgc ccatcatagt
     7141 gagcatcaag gcggtaagcc agtcccgacg cgccaactga cgcagacgca cgcgtcccag
     7201 ccaggcaatg ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat
     7261 cgccgggtat tccggtacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc
     7321 taacagggcg tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa
     7381 gttcaggaag cagacatgat tgctcccggc atcccgtcat tattagagag gatgcgggag
     7441 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     7501 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     7561 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     7621 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ctgtttgccg
     7681 ctctgtttgg tcagctctgc cgctaactgt gtcaatgtcc aggcactatc gcccgccagt
     7741 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     7801 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     7861 tccagtgctg ccggggcgct ggcgaggtag ttttcgctgt accagccgtt gcgcagcagg
     7921 gtgtaaacga tgccagaatc agccaacatt ttctccgtct cgatgtgctc atcggcgagg
     7981 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacaccagcc
     8041 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     8101 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     8161 tagtcagcct gacgcacggt aatgccttgt gctgccagag cctgggcttt tgccggatta
     8221 cgaacgatag ccactatttg gctgacagga accgttttct tcaaggattc aataacatag
     8281 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     8341 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgcaca aaaaggtaag
     8401 tatgaaatga gtcaggttag cctgtcgcaa caactgaaag agggtaatct ctttgcggaa
     8461 cagtgcccgt cgcgcgaggt gttgaaacac gtcaccagcc gttggggggt gttgattctg
     8521 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat cggcggggtg
     8581 agcgaaaaga tgcttgcgca gtcgttacag gcgttagaac aggatggttt tcttaaccgt
     8641 atcgcgtatc cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag
     8701 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     8761 gcggtgcggg atgaacgtgc ggcataactg ccggatgcgc tgagcttatc tggtctacgg
     8821 tgcatggctt gtagggcgga taagatgcgc cagcatcgca tccgccaata aatgccggat
     8881 gcggttgatg atttataggc cgaaaaagaa gtgttttact tactcaaatc cacctgataa
     8941 atcgcaaacc cgatatcatc ggtcgcgact ttattcatcg gatattgccc tttctcttta
     9001 ataaacgctg cggctttatc tgacggagaa gtttcgaaac ggatatccag tttcttatcg
     9061 ccagctatcg gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt
     9121 ttcgactcat cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa
     9181 gcgatatggc tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg
     9241 gcaacgagga acatggcgtt cggatcaatc ggcttgccat taaaggtcag gttcttaatc
     9301 ctttccgcat tggcattaat catctggcac tcgccgtcat aacgggcagg ctgggtaaca
     9361 tcaatctgat aattcacacc atcaatcaca tcaaagttat aggtgcggaa accatcccag
     9421 ttgatgagtg attgcggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag
     9481 cactccagcc actctttcac ctctttaccg ctggctttca ccacaatcag cgtattgggg
     9541 tagagataaa gatcggcggc attacggaag gtcaactggc ctttttccac ctccacatag
     9601 cttgccgggt catttttgcg accaccaact ttaaacggtg cggcagctga aagcaccggc
     9661 agttttgcca gatccggatc gccctgaatg taatgttcga catacgcttt ttgcgcgttg
     9721 ttcactactt gcacggtcgg atcgtcctgc accagcgcca gatagctata catattgtcg
     9781 gcagatttac cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt
     9841 gtttctacca gcttgctgtc ttccgccgcg agggattttt tattggcgat gtcgtaaatc
     9901 ggccgagctt ccgctttcgc ctgcgtcacc tgccatttac cgctgtcatt actgagttgt
     9961 aagtcgacca ccccaagatg atcgccccac atgcccggca ttaccgccgg aacaccattc
    10021 agcgtgcctt tggtgatatc agccccttcg atatcagcaa aatctttacc cgggaaaacg
    10081 gcgtgagcat gaccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact
    10141 gagttttccg ccattacttt atacggatcg gcagacagcc cggaatgcgc cagaacgaca
    10201 acgacatcgg cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata
    10261 tcattcaccg tcacttttcc ggataaatta gctttatccc agcccatgat ttgcggcggc
    10321 acgacgccaa tatagccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg
    10381 gtatctttaa ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg
    10441 gcatttacat aagggaattt cgctcccgcc agcgcatttt tcaggtaatc cagaccgtag
    10501 ttaaattcat gattgccgag tgtaccgacc gtataatcca gcgtattcag cgccttataa
    10561 accggatgaa catcacctgc ttttaatcct ttcgccgaca tgtaatcggc cagcggactc
    10621 ccctgaatca aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg
    10681 ttaatcaggc ttgccgtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa
    10741 tcgaaatcca tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc
    10801 gctgcattca cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc
    10861 agggacatcc ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat
    10921 agaacagtga agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc
    10981 gcattcatta acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag
    11041 gtggagaaat gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc
    11101 aggtctacga cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa
    11161 cggcagcgga tattgccgct cacaccgtta tcatggacgg tttacgtacg ctgacaccgg
    11221 atattccggt cctttctgaa gaagatcctc ccggttggga agtccgtcag cactggcagc
    11281 gttactggct ggtagacccg ctggatggta ctaaagagtt tattaaacgc aatggcgaat
    11341 tcaccgttaa cattgcgctc attgaccatg gcaaaccgat tttaggtgtg gtgtatgcgc
    11401 cggtaatgaa cgtaatgtac agcgcggcag aaggcaaggc ctggaaagaa gagtgcggtg
    11461 tgcgcaagca gattcaggtc cgcgatgcgc gcccgccgct ggtggttatc agccgttccc
    11521 atgcagatgc ggagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca
    11581 tcggctcttc gctgaaattc tgcctggtgg cggaaggaca ggcgcagctg tacccgcgct
    11641 tcggaccaac gaatatttgg gacaccgccg ctggacatgc tgtggccgca gctgccggag
    11701 cgcacgttca cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga
    11761 atcctgggtt cagagtgtct atttattaat taatttatgc agcaggtcca tcacttgctg
    11821 cacctcttcc tgagtaagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag
    11881 caccactaca gcggaacttt tttcatccag ctgccaggca ccgcgtgcga cgccattgct
    11941 atcaacaata aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac
    12001 aaacatgccg gaacccggaa ttgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg
    12061 atcgtgcggt aacttcgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc
    12121 agaggtgcga ccagcaatat gttgcagtac tcgcactttt cccactaact gcgcgctgtt
    12181 ccaggttttg tagctaaact gatctttatc aagcaccaac tcgccacgat cggtgatgcc
    12241 aatcggcggc actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag
    12301 caggcaggtg agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca
    12361 ggtgatccga ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc
    12421 aagtgtgatg ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact
    12481 gaagaccgcg cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag
    12541 ttatacgcgg tggaaacatt gcccggatag tctatagtca ctaggcatta aaatttgcgc
    12601 ctcataatag ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca
    12661 atgaaaattt tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc
    12721 cgtggacggt tatacatcaa ggacgttggc gcttttgaat ttgataaggg taagattctt
    12781 atcccaaaag tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt
    12841 atgcgtctgc aaacagagat ggcttaacca aagtgctatg cagtaataag acggctcctg
    12901 attcaggagc cgttgatgtt tctgggggtt acgcgacgct ttcttcttta tctttagcgt
    12961 caggcagttt tggcgaaagg gcggtggcct tgctgtcgat ccgggtcacc agcagctggt
    13021 cgatgcggta gttatcgata tccaccactt caaatttgta gccggcgaat ttcaccgaat
    13081 cggtgcgttt cgggatctta cgcagcataa acatcataaa gcctccgatg gtttcgtagt
    13141 tgcccgactg cgggaactcg tcaatatcca gcacgcgcat gacgtcgtca attggggtgc
    13201 cgccgtcaat cagccatgag ttctcatcac gggcgacaat ctgctcttcc agcccctgac
    13261 cgaccagatc gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc cccaccacca
    13321 gcgcgtactc gttcatgatc accgcgaagt cttcacctgc ggttttaaaa ctttccaacg
    13381 cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt gcgaatttgt acgccgctgt
    13441 tcagtgccag gctttggtta gccagcacgc ggttcagcag gtctttagaa tcgacataac
    13501 cgatgatgtg gtcaatatct tcattacaga cgaggaactt agagtgcgga tgttccgcca
    13561 ccttattctt caggctttgc tcatcttcgt ggagatcaaa ccaaatcacg ttttcacgcg
    13621 gtgtcattga agacggaacg gtacgggatt ccagctcaaa gacgttttca atcagctcgt
    13681 gttcctgttt acgtaacacg cccgccagcg caccggcttc cactaccgcg tagatgtcat
    13741 cagaagtgat gtcatcttta cgtaccattg gcagtttgaa aatacggaag atcatgttcg
    13801 ccaggccgtt gaagaaccac accagcgggg tgcaaacgta caggcagaag cgcatcgggt
    13861 tgatgatacg caaagccacc gcttctggcg caatcatacc gatgcgtttc ggggttaaat
    13921 ccgcaaacag gataaacatg ccagtcacta acgagaaaga gagaataaag ctcagttgct
    13981 cagagagttc tgccgacata tagcgggaga acaggctgtg aaaagctgga gaaaatgccg
    14041 catcaccgac gataccgccg agaatcgcaa ctgcgttcag accgatttgg accaccgtaa
    14101 agaacatgcc gggattttcc tgcatattca gaacgcgttg ggcatttata ttgccttcat
    14161 cagccagcag tttaagtttg attttgcgtg aggcggcaag tgagatctca gacatcgaga
    14221 agaacgcact tacagcgatc aagcagagta tgactaaaat actgtttaac atatcttatc
    14281 cgacctttca ggccggatcc tcggaagggg aagttgatta atttgtgtga aatacacatt
    14341 gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc gtaaggtact
    14401 gtaaagtcgc cagagggtta aaattcagac tgctgataaa tgcaatactg cctgatgcgc
    14461 tatgccgatc aggcctacgc agtccctgca atatattgaa tttggacgat tttgtaggcc
    14521 tggtaaggca ttcacgccgc attcggcata aacaaaacgc actttatcaa caatctacat
    14581 ttgtacccgt aacgctatgc ttccggtggc agacagacgc caattccgcc aattccacag
    14641 taaccatacg ggtttttatg cagatattgc tggtggtcat cttcggcata ataaaacggt
    14701 gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat cggcggcaag catcgccgcc
    14761 tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt ctggggtcag cggataaatc
    14821 gctgaacgat actgcgtgcc gtggtcattg ccctgacgca ttccctgggc gggatcgtga
    14881 ttctcccaaa atacctgtag caactgctca tagctgatga cagaaggatc gtaaacaatg
    14941 cgtaccgctt cggcatgacc cgtatcaccg gagcacactt cccgataagt cggatttggc
    15001 gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg ccagaacaga
    15061 cgctccacac cccagaaaca acccatcgca aaaatggcaa tctccattcc gtcaggtaca
    15121 ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta cgggcatcgg ggtgttacgt
    15181 ccaggcaggg catcggcggg ggaaaccaga tgctttttat caaataaact catggtgtcg
    15241 ctctcccgaa aatcggtcat tggggttaag gttgtaacaa gaggcgtatt tgcacacaat
    15301 aaccaccgtg aataggtcta aagtaaaaca taagaaatat ttggggttta gtctgctttt
    15361 taatccatat tactggattt ttgttaagcc gtttaacggc gttccagggg caggaaaaaa
    15421 ggatattcag gagaaaatgt gcgctatatc cgacagttat gctgtgtaag cttactctgc
    15481 ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt atcgggacag
    15541 ctggaaaaga acgttcgtgc gcagctttct acgattgaaa gtgatgaagt gacgccagac
    15601 cgtcgctttc gcgcacgcgt cgatgatgcc atccgcgaag gtctgaaagc gctgggttat
    15661 taccagccga ccattgaatt tgatctccgt ccaccgccaa agaaagggcg gcaggtattg
    15721 atcgccaaag tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt ggtattgcgc
    15781 ggcggcgcgc ggaccgataa agactatttg aaattgctcg atactcgccc ggctattggc
    15841 acggtactga accagggcga ttatgaaaat ttcaaaaagt ccttaaccag cattgcgttg
    15901 cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc tgggcattgc gctcggcctg
    15961 cataaagcct tctgggatat tgattataac agtggcgaac gttaccgctt tgggcatgtg
    16021 acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc gtttaaagag
    16081 ggcgatgagt acgaatcgaa agatctggcg gaacttaacc gtcgactttc tgctaccggc
    16141 tggtttaact cggtcgtagt ggctccacaa tttgataaag cgcgcgaaac gaaagtatta
    16201 cccttgacgg gcgtggtttc gccgcgaact gaaaacacca tcgaaaccgg ggtcggttac
    16261 tctacggacg tgggaccgcg cgtgaaagct acgtggaaaa agccgtggat gaactcttat
    16321 ggtcacagtc tgaccaccag taccagtatt tccgcgccgg aacagaccct cgacttcagc
    16381 tataaaatgc cgctgctgaa gaatccactg gaacaatatt atttggtgca gggcggtttt
    16441 aagcgcactg acctgaacga taccgaatct gactccacta cgctggtggc ttctcgctac
    16501 tgggatctct ccagcggctg gcagcgtgcc attaacctgc gctggagtct cgaccacttt
    16561 actcagggtg aaattaccaa caccacgatg ctgttttatc ctggggtgat gattagccgc
    16621 acgcgttctc gtggtggcct gatgccaacc tggggcgact cgcaacgcta ctctatcgac
    16681 tactccaaca cggcctgggg ctcagatgtc gatttctccg ttttccaggc gcagaacgtc
    16741 tggatccgca cactgtacga tcgccatcgt ttcgttacac gcggcacgct gggctggatt
    16801 gaaaccggtg atttcgacaa agtaccgccg gatctgcgtt ttttcgccgg gggcgatcgc
    16861 agtattcgcg gctacaaata caaatctatc gccccgaaat acgccaacgg tgacctgaaa
    16921 ggggcctcga agttgataac cggatcgctg gaataccagt acaacgtgac cggaaaatgg
    16981 tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg atattcgccg cagcgacttt
    17041 aaaaccggta ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat caaactcgat
    17101 tttgccgtac cggtcgcgga taaagacgaa cacgggttac agttttacat cggtctgggg
    17161 ccagaattat gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt atcttactgt
    17221 tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg cctgcatctt gtatttaaag
    17281 cggcggatcg ctgggtgcca ggactggata ttggcaaggt caccggcggc tggcgcgatc
    17341 tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc ggtaaaagcg ggtaatctgc
    17401 atctggcggt cggtcttgag tgcctgtgga acagtagcgt ttgtattaat gacctggcgc
    17461 tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa caggttgaag
    17521 aagaggaaga tagcggtccg ctggatctct ccacgccgta tcccatcacc ctgacacggg
    17581 tggcgctgga caacgtcaac atcaagattg atgacaccac ggtgtcggtg atggacttca
    17641 cctccggcct gaactggcag gagaaaaccc tgaccctgaa accgacgtcg ctgaaaggcc
    17701 tgctgattgc tctgccgaaa gtggcggaag tggcgcagga agaagtggtc gaaccgaaaa
    17761 ttgaaaatcc gcagccggag gaaaagccgc tcggcgaaac gctgaaagat ctcttttctc
    17821 gcccggtatt gccggaaatg accgacttgc atttgccgct taacctgaac attgaagagt
    17881 ttaaaggcga gcagttacgc gtgacgggcg acacggacat caccgtgcgc accatgctgc
    17941 tgaaagtgag cagcattgac ggcaatacta aactggacgc cctggatatc gattccagtc
    18001 aagggagcgt caacgccagc ggcacggcgc agctgtcaga caactggccg gtggatatca
    18061 ccctcaacag cacactgaac gtggagccgt tgaaaggtga aaaagtgaag ctaaaagtgg
    18121 gcggcgcgct gcgcgaacag ctggagattg gcgttaatct ttccggtccg gtggatatgg
    18181 atttacgcgc ccagacgcga ctggcggaag ccggattgcc actcaacgtg gaagtgaaca
    18241 gcaaacagct ttactggccg ttcaccggtg agaagcagta tcaggcggat gatctgaaac
    18301 tgaaacttac cggcaaaatg accgattaca cgctctctat gcgtacggca gtgaagggac
    18361 tggagatccc gccagccacc attacccttg atgccaaagg taatgaacag caggtcaatc
    18421 tcgacaaact caccgtcgcg gcgctggaag ggaaaactga actcaaggcg ttgctcgact
    18481 ggcagcaggc cattagttgg cgcggtgagc taacgcttaa cggcattaac accgccaaag
    18541 agatcccaga gtggccgtcg aaactcaatg gcttgattaa aacccgcggt agcctgtacg
    18601 gcggcacctg gcagatggag gtgccagaac tgaagctgac cggtaacgtc aaacagaaca
    18661 aagtgaacgt tgacggcacg ctgaaaggca acagttatat gcagtggatg atcccagggc
    18721 ttcatctgga actggggcca aacagtgccg aagtgaaagg cgagctgggg gtaaaagatc
    18781 tcaatcttga tgccaccatc aacgcgccgg ggctggataa cgcgctgccg gggcttggcg
    18841 gtacagcgaa agggctggtg aaagttcgcg gcacggtgga agcgccacaa ctactggcgg
    18901 acatcaccgc gcgcggcctg cgctggcagg agctaaccgt ggcgcaggtt cgcgtggaag
    18961 gcgatatcaa atccaccgtt cagattgcgg gtaaactcga cgtgcgcgtt gagcaaattt
    19021 cacagccgga tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc gaaaaacagc
    19081 acgagttaca gttgcggatt cagggcgagc cggtttccgg gcagcttaat ctggcaggaa
    19141 gttttgatcg caaagaagaa cgctggaagg gaactcttag caatacccgc ttccagacgc
    19201 cggtcggccc gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat aaggagcaaa
    19261 aaatcagcat cgggccacac tgctggctta acccgaatgc ggaactgtgc gtgccgcaaa
    19321 ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct caaccgcttc gacctcgcca
    19381 tgctgaaacc gtttatgcca gaaaccactc aggccagcgg tatcttcacg ggtaaagcgg
    19441 atgttgcctg ggacaccacg aaagaggggc tgccgcaggg cagtatcacc ctttcggggc
    19501 gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct gcccgtggcg tttcagacac
    19561 tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt gggctggacc atccgcctga
    19621 ccaataacgg ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc cgccgtaatc
    19681 ttggtggcaa cgtcaatatc cgtaacttca accttgcgat gataaacccc atctttaacc
    19741 gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg tctgggtggt gatgtgcaaa
    19801 gcccgcagtt gtttggtcag cttcaggtta cgggtgtgga tatcgacggc aactttatgc
    19861 cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa tggtatgcgc tcgacgcttg
    19921 ccggtacagt acggacccag cagggtgaaa tctacctgaa cggtgatgcc gactggagcc
    19981 aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg atcaccgtgc
    20041 cgccgatggt acgaatggat gtatcgccag atgttgtctt cgaggctaca ccaaacctgt
    20101 ttaccctcga tggtcgcgtg gatgtcccgt gggcgcgcat cgtggtgcac gatctgccgg
    20161 aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa cgataacctg caacctgaag
    20221 agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat tgtccacgtt ggcaacaatg
    20281 tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg cgatcttaac gtcgttcagg
    20341 acaaacaagg gctgggcctg aacgggcaga tcaacatccc tgaagggcgc ttccatgcct
    20401 atggtcagga tctgattgtg cgtaaaggtg agttactgtt ctctggtccg ccggatcaac
    20461 cgtatcttaa tattgaagct attcgtaacc cggatgctac agaagacgac gtaatcgccg
    20521 gagttcgcgt cactggtctg gcggacgaac cgaaagcgga gatcttctct gacccggcga
    20581 tgtcgcaaca agctgcattg tcttatttgc tacgtggaca agggctggag agcgatcaga
    20641 gcgacagtgc ggcaatgacc tcgatgctga ttggtctggg ggttgcgcaa agtggccaga
    20701 ttgtgggtaa aatcggcgag acgtttggcg taagcaattt agcgctcgac acccagggag
    20761 taggcgactc ctcccaggta gtggtcagcg gctatgtatt gccaggtctg caagtgaaat
    20821 atggcgtggg tatatttgac tctatagcaa cactcacgtt acgttatcgc ctgatgccta
    20881 agctatatct ggaagccgtg tctggtgtag accaggcact ggatttgctc tatcagttcg
    20941 agttttagca atgcgaatat ttgtctacgg cagtttacgc cacaaacaag gcaacagtca
    21001 ctggatgacc aatgcccagt tactgggcga tttcagtatc gataactacc agttgtatag
    21061 cctgggccac tatccaggcg cagttccggg gaacggaaca gtacacggtg aagtttatcg
    21121 tattgacaac gccacgctgg ccgaacttga tgccttgcgc accaggggcg gtgaatacgc
    21181 gcgccagttg attcagacgc cgtacgggag tgcatggatg tacgtttatc aacgacccgt
    21241 cgatggatta aagctaattg aaagcggcga ctggttagac agggataagt aaccatatgc
    21301 atacgccacc ttcgggtggc gttgtttttt gcgagacgac tcgcattctg ttttgtaatt
    21361 ccctcaccct ttgcttttct ctccgagccg ctttccatat ctgttaacgc ataaaaaact
    21421 ctgctggcgt tcacaaatgc gcaggggtaa aacgtttcct gtagcaccgt gagttatact
    21481 ttgtataact taaggaggtg cagatgcgta ttaccataaa aagatggggg aacagtgcag
    21541 gtatggtcat tcccaatatc gtaatgaaag aacttaactt acagccgggg cagagcgtgg
    21601 aagcgcaggt gagcaacaac caactgattc tgacacccat ctccaggcgc tactcgcttg
    21661 atgaactgct ggcacagtgt gacatgaacg ccgcggaact tagcgagcag gatgtctggg
    21721 gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa agtgaatttg aacggggaga
    21781 cattgtgctg gttggctttg atccagcaag cggccatgaa cagcaaggtg ctggtcgacc
    21841 tgcgcttgtg ctctccgttc aagcctttaa tcaactggga atgacgctgg tggcccccat
    21901 tacgcagggc ggaaattttg cccgttatgc cggatttagc gttcctttac attgcgaaga
    21961 aggcgatgtg cacggcgtgg tgctggtgaa tcaggtgcgg atgatggatc tacgcgcccg
    22021 gctggcaaag cgtattggtc tggctgcgga tgaggtggtg gaagaggcgt tattacgctt
    22081 gcaggcggtg gtggaataag gtgtgtttat ttatcgcggg cataaaaaaa cccttactaa
    22141 ccgaagctcg gcgttcaggg ttattgcgct agaagaactt atttattctt tgcgcgctcg
    22201 aaggaagcaa cgatttcagc tttagcggct tctgcgtttt cccaaccttc aactttcacc
    22261 cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt gagcgatctg cgctttcagc
    22321 agctctggca gatcgttaac gtctttaatg tgatcgtatt ctttgctcag cttgctgtgc
    22381 ggaaccgcaa ccagtttcgc atcttcaccg gcttcgtcgg tcattttcag aacgccaacc
    22441 ggacggcaac ggatcacaga acccggctgc agcgggtacg gagtcgggac cagtacgtca
    22501 accgggtcac cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca cggatagaac
    22561 atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc gatttcgtat
    22621 ttgatcggat ctgcgttagc cgggatctca ataacaacgt agatgtcttc cggcagatct
    22681 ttacccgcag ggacgttgag taagctcatg tctgtttcct ttaaaaatat gtggcaaaca
    22741 agtgccgagt attatagcca actcgcgccg aatgtctttg cttgttttcg tcttcatttt
    22801 cctttttcgc cagttttaag acggtataca gagcaggaaa atccataacc gtagccgcat
    22861 ttttcatagt gagatgaaag cgattacaaa cttgtgatta acgttttatt tacttttttg
    22921 aagtgtgatg taacgcaatc cgttacataa cgaattgtct atagtttttt cgcgaacatc
    22981 ttttcaccaa taataattac cccgacgagg acaaccctat gtggaaacgc ttacttgtag
    23041 tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc cgctccatta accgtaggat
    23101 tttcgcaggt cggatcggaa tccggctggc gcgctgcaga aaccaatgtg gcgaaaagtg
    23161 aggccgaaaa acgcggaatt acgttgaaaa ttgccgatgg tcagcaaaag caggaaaacc
    23221 agattaaagc ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt atcgctccag
    23281 tggttgcgac gggttgggaa ccggtattaa aagaggcgaa agatgccgaa atcccggtct
    23341 tcttgcttga ccgttccatc gatgtgaaag acaaatctct ctatatgacc accgtcactg
    23401 ccgacaacat cctcgaaggc aagttgattg gtgactggct ggtaaaagaa gtgaatggca
    23461 aaccatgcaa cgtggtggaa ctgcagggca ccgttggggc cagcgtcgcc attgaccgta
    23521 agaaaggctt tgccgaagcc attaagaatg cgccaaatat caaaatcatc cgctcgcagt
    23581 caggtgactt cacccgcagt aaaggcaaag aagttatgga gagctttatc aaagcggaaa
    23641 acaacggcaa aaacatctgc atggtttacg cccataacga tgacatggtg attggtgcaa
    23701 ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga tatcctcacg ggttccattg
    23761 acggcgtacc ggatatctat aaagcgatga ttgatggcga agcgaacgcc agcgttgaac
    23821 tgacgccgaa catggcaggc cccgcttttg acgcgctgga gaaatacaaa aaagacggca
    23881 ccatgcctga aaagctgacc ctgaccaaat ccacccttta tctgcctgat accgcaaaag
    23941 aagagttaga gaagaagaaa aatatggggt attgagggtt gctatgcctg atgccgattc
    24001 gtaggccgga taaggcgctc gcgccgcatc cggcgatggt gcactgaagc ctgatgcgac
    24061 gcttaccgcg tcttatcatg cctactggga gcacgcttta caccggggga aaccatgacg
    24121 accgaccaac accaggagat cctccgcacc gaaggattaa gtaaattttt ccccggcgtc
    24181 aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc gctgcttggt
    24241 gaaaacgggg cgggaaaatc aacgctaatc aaagcattaa ctggtgtata ccacgccgat
    24301 cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc ccatgcacaa
    24361 caactcggta tcggcaccgt ctatcaggaa gtcaacctgc tacccaatat gtcggttgct
    24421 gataatctat ttataggccg cgaacccaaa cgtttcggcc ttctacgccg taaagagatg
    24481 gaaaagcgcg ccaccgaact gatggcatct tacggtttct ccctcgacgt gcgcgaaccg
    24541 cttaaccgtt tttcggtggc gatgcagcaa atcgtcgcca tttgccgggc tatcgatctc
    24601 tccgccaaag tgctgatcct cgatgaaccc accgccagcc tcgacaccca ggaagtggag
    24661 ctattatttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgct ctttgtcact
    24721 cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgtcttacg caacggcagt
    24781 ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa aatgatgctg
    24841 gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt gagcgacaaa
    24901 cccgttgccg cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt tgatctcgaa
    24961 gtgcgccccg gcgagatcgt cggtctggct ggcttgctgg gatccggacg taccgaaacc
    25021 gccgaagtga tcttcggtat caaacctgct gacagcggca cggcgttgat caaaggcaaa
    25081 ccgcaaaccc tgcgatcgcc acatcaggct tcagtactgg gcatcggatt ctgcccggaa
    25141 gacaggaaaa ccgatggcat catcgctgcc gcctcggtgc gggaaaatat catcctcgct
    25201 ctccaggccc agcgcggctg gctacgaccc atttcccgca aagagcagca agagattgcc
    25261 gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc gattgaattt
    25321 ctctctggcg gcaatcagca aaaagtgttg ctttcacgtt ggctactgac acgaccgcaa
    25381 tttctgatcc tcgacgagcc aacccgcggc attgatgttg gtgcccacgc cgagatcatc
    25441 cgcctgattg aaacgctgtg tgctgacggt ctggcgctgt tggtgatctc ctccgaactg
    25501 gaagagctgg tgggctatgc cgatcgggtg attatcatgc gcgatcgcaa acaggtggcg
    25561 gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc ggcgtaagga
    25621 gaacagtgtg atgcctcaat ctctcccgga cactacgccg ccgaaaaggc gctttcgctg
    25681 gccaacggga atgccgcagc tggcggcgct attgctggtg ctgctggtcg atagcctggt
    25741 agccccgcat ttctggcagg tggtgcttca ggatgggcgt ttgttcggta gccctatcga
    25801 cattcttaac cgtgcagctc ccgttgcgtt actggcgatt ggtatgacgc tggtgatcgc
    25861 caccggtggg attgatctct ccgtaggggc ggtgatggct atcgccggag ccacaacggc
    25921 tgcgatgacg gtcgcgggat tcagcctgcc gattgttttg ttaagcgccc tgggcaccgg
    25981 catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc agccgtttgt
    26041 cgccaccctg atcctgatgg tcgccgggcg cggcgtggcg caactgatca ccgccgggca
    26101 gatcgtcacg tttaactcgc cggatctctc atggtttggc agcggatcgc tgttgttcct
    26161 gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt tgacccgcaa
    26221 aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg caaaaaatgc
    26281 cggggtaaac acgcggatca tcgtcatgct cacctacgtg ttgagcgggc tgtgtgcggc
    26341 gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaata acgccgggtt
    26401 atggctggag ctggacgcca ttcttgcggt ggtgattggc ggcggatcgc tgatgggcgg
    26461 gcgctttaac ctgctgctct cagtcgtggg ggcgctgatt attcagggga tgaacaccgg
    26521 aattttactt tctggctttc cgccggagat gaaccaggtc gtaaaagcgg tggtggtgct
    26581 ttgtgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag tacgtagcca
    26641 tgataaaacg taacttgccg ctgatgatca ccatcggcgt ctttgtgctg ggctatctct
    26701 actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat atcctgaccg
    26761 ataacgcctt tcttgggata atcgccgttg gcatgacctt tgtgatcctc tctggcggta
    26821 tcgatctctc cgtcggttcg gtgatcgcct ttactggcgt gtttctggca aaagtgattg
    26881 gcgatttcgg cctctcgccg ctgctggcgt ttccgctagt gctggtgatg ggctgtgcct
    26941 ttggcgcatt tatggggctt ttgatcgacg ccctgaagat cccggcattt atcattaccc
    27001 tggcaggaat gttctttttg cgcggcgtca gctatctcgt ttcagaagag tcgatcccga
    27061 taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct ggcggcggtc
    27121 gcttaagtgc gatgggactg ctgatgctgg cggtggtggt tatcggcata ttcctcgcgc
    27181 atcgtacccg ttttggtaat caggtatacg ccattggcgg caacgcaacg tcggcaaacc
    27241 tgatggggat ttctacccgc agcaccacta ttcgcattta tatgctctcc accggactgg
    27301 caacgctggc ggggattgtc ttctcgattt atacccaggc cggatatgcg ctggcgggcg
    27361 taggtgtgga actggatgct atcgcctcag tggtaattgg cggtacgctt ttgagcggtg
    27421 gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcaggggcta attcagactt
    27481 atatcaactt tgacggtacg ctgagttcct ggtggacgaa aatcgccatc ggcattttgc
    27541 tgttcatttt tattgcctta cagcgtggat taacggtgct gtgggagaat cgtcagagtt
    27601 cgccagtgac aagagtcaat attgcgcagc gataaaaacg cctctccgtg tggagaggcg
    27661 caggagatta cgcgtccggg aattcacgga taaagcgttc gacatcttca accatatggt
    27721 cgttgccgac aaagaacgaa cggcgctggt gcagagtttc cgggatgata tccagaatac
    27781 gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc atcgggttgc
    27841 actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg tagagataaa
    27901 taccgccttt cagcaggtta cggtggaaat ccgcgaccag tgaaccgata taacgtgagg
    27961 tatacgggcg gttggtggat ttatcttctt cctggcagaa tttaatgtac ttcttcaccc
    28021 cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct ttctccggga
    28081 agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt
    28141 gaacaccgca tccggtggtg taaaccagca tggtagagga gccgtatacc acgtaacctg
    28201 ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccaacag
    28261 gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg
    28321 acgagccatc cagggggtcc atcagcacca cgtattttgc gtgttcacag ccttcaaaga
    28381 cgacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt gctttcagtg
    28441 cggctttcag tttttcatta gcgaacaagt cgagtttctg ctgaacctcg ccctgcacgt
    28501 tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga
    28561 taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag
    28621 aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc
    28681 aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat gttgcgctca
    28741 ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact tgcccgacat
    28801 atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt catattttag
    28861 gaatttgtgg cacatttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag
    28921 taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca
    28981 ttgagctgat tcagggttac gatgccagcc agctcgatcc gcagccggat ctggtgatta
    29041 ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc
    29101 cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg
    29161 ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg attctggaac
    29221 agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg
    29281 cgcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac tgcgccttct
    29341 tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc aacaaccttg
    29401 agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag ttccaccatc
    29461 tggtgcgtat cgttccgggg cagggccgta ttatctggcc ggaaaatgac atcaacctga
    29521 aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggtgag cagggtcact
    29581 ggcaggcgaa aaagctgacc actgacgctt ctgaatggga agtcttgctg gatggcgaaa
    29641 aagtgggcga agtgaaatgg tcgctggtgg gcgaacataa tatgcacaat ggcctgatgg
    29701 cgattgcggc ggctcgccat gttggtgtag cgcctgcaga tgccgctaac gcgctgggtt
    29761 cgtttattaa cgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc gtgacggtat
    29821 atgacgattt tgcccatcac ccgacggcaa ttctggcaac gcttgcggcg ctgcgtggca
    29881 aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat accatgaaaa
    29941 tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa gtcttcctgc
    30001 tgcaaccggc gtatattccg tggcaggtgg cagaagtggc agaagcctgc gttcagcctg
    30061 cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc gctcagcctg
    30121 gcgaccatat tctggtgatg agcaacggcg gttttggtgg gatccatcag aaactgctgg
    30181 atggtctggc gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg atatagtgtg
    30241 cgtaggccgg ataaggcgtt cacgccgcat ccggcatttg agcatagtgc ctgatgcgac
    30301 gcttgatgcg tcttatcagg cctacaggtg caccgcatcc ggcaaaccat actcacatca
    30361 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
    30421 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agagtacgca
    30481 gctgctgacg atcggcatcg tgccacagat ttaatacctc agcgatggcg tcatcacctt
    30541 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
    30601 gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc tggcgcagca
    30661 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
    30721 caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
    30781 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
    30841 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
    30901 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
    30961 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
    31021 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
    31081 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
    31141 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
    31201 cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg catcatccac
    31261 cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata ttgcccgtta
    31321 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
    31381 agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
    31441 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatt accaataccg aaggtggcag
    31501 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
    31561 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
    31621 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
    31681 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
    31741 gaaagcgcca gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
    31801 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
    31861 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
    31921 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
    31981 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
    32041 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
    32101 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
    32161 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
    32221 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
    32281 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
    32341 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
    32401 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    32461 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
    32521 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
    32581 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgaaga
//
LOCUS       DAZKGS010000037        32442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKGS010000037 DAZKGS010000000
VERSION     DAZKGS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            10..612
                     /gene="yebB"
                     /locus_tag="ACHOQY_001729"
     CDS             10..612
                     /gene="yebB"
                     /locus_tag="ACHOQY_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9677850.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(614..1135)
                     /gene="ruvC"
                     /locus_tag="ACHOQY_001730"
     CDS             complement(614..1135)
                     /gene="ruvC"
                     /locus_tag="ACHOQY_001730"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9677851.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1170..1910)
                     /gene="yebC"
                     /locus_tag="ACHOQY_001731"
     CDS             complement(1170..1910)
                     /gene="yebC"
                     /locus_tag="ACHOQY_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9677852.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1939..2391)
                     /gene="nudB"
                     /locus_tag="ACHOQY_001732"
     CDS             complement(1939..2391)
                     /gene="nudB"
                     /locus_tag="ACHOQY_001732"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9677853.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2509..4281)
                     /gene="aspS"
                     /locus_tag="ACHOQY_001733"
     CDS             complement(2509..4281)
                     /gene="aspS"
                     /locus_tag="ACHOQY_001733"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9677854.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4591..5157
                     /gene="yecD"
                     /locus_tag="ACHOQY_001734"
     CDS             4591..5157
                     /gene="yecD"
                     /locus_tag="ACHOQY_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9677855.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5154..5972
                     /gene="yecE"
                     /locus_tag="ACHOQY_001735"
     CDS             5154..5972
                     /gene="yecE"
                     /locus_tag="ACHOQY_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9677856.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6025..6420
                     /gene="yecN"
                     /locus_tag="ACHOQY_001736"
     CDS             6025..6420
                     /gene="yecN"
                     /locus_tag="ACHOQY_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9677857.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6461..7204
                     /gene="cmoA"
                     /locus_tag="ACHOQY_001737"
     CDS             6461..7204
                     /gene="cmoA"
                     /locus_tag="ACHOQY_001737"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9677858.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7201..8172
                     /gene="cmoB"
                     /locus_tag="ACHOQY_001738"
     CDS             7201..8172
                     /gene="cmoB"
                     /locus_tag="ACHOQY_001738"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9677859.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8337..10766)
                     /gene="torZ"
                     /locus_tag="ACHOQY_001739"
     CDS             complement(8337..10766)
                     /gene="torZ"
                     /locus_tag="ACHOQY_001739"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9677860.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10791..11891)
                     /gene="torY"
                     /locus_tag="ACHOQY_001740"
     CDS             complement(10791..11891)
                     /gene="torY"
                     /locus_tag="ACHOQY_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9677861.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12279..13025)
                     /gene="cutC"
                     /locus_tag="ACHOQY_001741"
     CDS             complement(12279..13025)
                     /gene="cutC"
                     /locus_tag="ACHOQY_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9677862.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(13039..13605)
                     /gene="yecM"
                     /locus_tag="ACHOQY_001742"
     CDS             complement(13039..13605)
                     /gene="yecM"
                     /locus_tag="ACHOQY_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9677863.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13821..15554
                     /gene="argS"
                     /locus_tag="ACHOQY_001743"
     CDS             13821..15554
                     /gene="argS"
                     /locus_tag="ACHOQY_001743"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9677864.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15596..15640)
                     /gene="yecV"
                     /locus_tag="ACHOQY_001744"
     CDS             complement(15596..15640)
                     /gene="yecV"
                     /locus_tag="ACHOQY_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFP9677865.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            15731..16219
                     /gene="yecT"
                     /locus_tag="ACHOQY_001745"
     CDS             15731..16219
                     /gene="yecT"
                     /locus_tag="ACHOQY_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP9677866.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(16339..16731)
                     /gene="flhe"
                     /locus_tag="ACHOQY_001746"
     CDS             complement(16339..16731)
                     /gene="flhe"
                     /locus_tag="ACHOQY_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9677867.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16731..18809)
                     /gene="flhA"
                     /locus_tag="ACHOQY_001747"
     CDS             complement(16731..18809)
                     /gene="flhA"
                     /locus_tag="ACHOQY_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9677868.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18802..19950)
                     /gene="flhB"
                     /locus_tag="ACHOQY_001748"
     CDS             complement(18802..19950)
                     /gene="flhB"
                     /locus_tag="ACHOQY_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9677869.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(20152..20796)
                     /gene="cheZ"
                     /locus_tag="ACHOQY_001749"
     CDS             complement(20152..20796)
                     /gene="cheZ"
                     /locus_tag="ACHOQY_001749"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9677870.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20807..21196)
                     /gene="cheY"
                     /locus_tag="ACHOQY_001750"
     CDS             complement(20807..21196)
                     /gene="cheY"
                     /locus_tag="ACHOQY_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9677871.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(21211..22260)
                     /gene="cheB"
                     /locus_tag="ACHOQY_001751"
     CDS             complement(21211..22260)
                     /gene="cheB"
                     /locus_tag="ACHOQY_001751"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9677872.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(22263..23123)
                     /gene="cheR"
                     /locus_tag="ACHOQY_001752"
     CDS             complement(22263..23123)
                     /gene="cheR"
                     /locus_tag="ACHOQY_001752"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9677873.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(23142..24746)
                     /gene="tap"
                     /locus_tag="ACHOQY_001753"
     CDS             complement(23142..24746)
                     /gene="tap"
                     /locus_tag="ACHOQY_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP9677874.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(24792..26453)
                     /gene="tar"
                     /locus_tag="ACHOQY_001754"
     CDS             complement(24792..26453)
                     /gene="tar"
                     /locus_tag="ACHOQY_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP9677875.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(26598..27101)
                     /gene="cheW"
                     /locus_tag="ACHOQY_001755"
     CDS             complement(26598..27101)
                     /gene="cheW"
                     /locus_tag="ACHOQY_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP9677876.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(27122..29086)
                     /gene="cheA"
                     /locus_tag="ACHOQY_001756"
     CDS             complement(27122..29086)
                     /gene="cheA"
                     /locus_tag="ACHOQY_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9677877.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(29091..30017)
                     /gene="motB"
                     /locus_tag="ACHOQY_001757"
     CDS             complement(29091..30017)
                     /gene="motB"
                     /locus_tag="ACHOQY_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9677878.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(30014..30901)
                     /gene="motA"
                     /locus_tag="ACHOQY_001758"
     CDS             complement(30014..30901)
                     /gene="motA"
                     /locus_tag="ACHOQY_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9677879.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(31028..31606)
                     /gene="flhC"
                     /locus_tag="ACHOQY_001759"
     CDS             complement(31028..31606)
                     /gene="flhC"
                     /locus_tag="ACHOQY_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9677880.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(31609..31959)
                     /gene="flhD"
                     /locus_tag="ACHOQY_001760"
     CDS             complement(31609..31959)
                     /gene="flhD"
                     /locus_tag="ACHOQY_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9677881.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7670 a         8800 c         7873 g         8099 t
ORIGIN      
        1 aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggtgatat cgtctttaca
       61 tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaactgctg gagtaatcac
      121 gtcgggatca ttatcggtca taacggtgaa gactttctgg ttgcagaaag ccgtgttccc
      181 ctctcaacca tcactacgct atcccgtttt attaaacgct ctgctaatca acgctatgct
      241 ataaagcgat tagacgccgg gctgacagaa caacagaaac aacgaattgt tgaacaggtt
      301 ccctcccggc tacgcaaact ttaccacacg ggttttaaat acgaatcttc ccgccagttc
      361 tgttcaaaat ttgtctttga tatttataaa gaggcgctat gtattccggt gggtgaaata
      421 gagacgtttg gacaactgtt aaatagcaat ccgaatgcaa aactcacttt ctggaaattc
      481 tggttcttag gttctattcc gtgggagcgt aaaaccgtca cgccagccag tttgtggcat
      541 catccgggtt tggtgttgat tcacgcggag ggagttgaaa cgcctcagcc tgaactgacc
      601 gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat tcgctcatct
      661 gcattgcatt ctggctgacg tgacaatggg taatcgcgat agctaacgca tcagcggcat
      721 ccgcttgtgg attagcgggc agtttcagca aggtgcggac catatgctgc acctggcttt
      781 tctcggcgct accaatacct accaccgttt gctttacctg acgtgccgcg tattcaaata
      841 ccggcaattc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgg cccagtttca
      901 gggctgagtc agcgttcttc gccataaaga cttgttcaat ggcgaaataa tcaggctgga
      961 actgggtgat gatttccgtc acgcccgcat agatgagctt cagacgagac ggtaaatcat
     1021 ccactttggt gcgtatgcat ccactaccca ggtaggacag ttgcctgcct acctggcgga
     1081 tgacgccata gccggtcacg cgcgaacccg gatcaatgcc gagaataata gccatcacgc
     1141 gtctccgttt tgctgtttag caggcctcat cagagagtcg ctgcaacctc atcagagatt
     1201 tcaccgttat ggtaaacttc ctgcacgtcg tcgcaatctt ccagcatatc gatcagacgc
     1261 atcagtttcg gtgcggtttc tgcatccata tcagctttgg tggacgggat catggaaact
     1321 tccgcgctgt ctgctttcag acctgccgct tccagagcgt cgcgcacttt acccatttct
     1381 tcccatgcgg tgtagacatc aatcgcgccg tcatcatagg tcacaacgtc ttcagcacct
     1441 gcttccaaag ctgcttccat gatggtgtct tcatcgcctt tctcgaagga gatcacgcct
     1501 tttttgctga acagataggc tacggaacca tcagtaccga ggttaccgcc acatttgctg
     1561 aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca ttcaatcatg
     1621 attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt tgcatcatca
     1681 tcaccgccca caccacgtgc aattgcgcgg ttcagtgtgt cgcgggtcat gttgttagac
     1741 agtgctttat caattgctgc acgcagacgc gggttagcgt ccggatcgcc accgcccagc
     1801 ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg ctttgcatcc
     1861 tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat aaaaatatct
     1921 ccagatagcc ctgcctgttc aggcagcgtt aatgacaaac tgttcaatcg cctgccggtt
     1981 gctccaggac ttagtgagcg ccgccgcagc aggcgcatca agccacttgt aagccagatg
     2041 ttcagtgaaa acgatctgcc gctcgtgcgg aagcgcaaga cagaaccatg attccgtatt
     2101 acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa actctaccgt
     2161 gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga cctcttcctt
     2221 tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc cggttaccga
     2281 ctgccagaaa tcgggatcgt cacgccgctg caacatcagc acccgtttcg tatcttgtgc
     2341 atagatgacc actaagatcg aaacgggacg cttatacact ttatccttca cgctgcctct
     2401 tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg gagttcactc
     2461 agttgccgcc ttgatgcaac gcgaatgatt tcgtgtattt gagtcatatc agttattctc
     2521 agccttcttc acaacctgaa tgctcagctc agccagtgca gtcgggttag caaagctcgg
     2581 tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa cgtcacggat
     2641 attgtcggtg ccagtcagca gcatggtcag acggtcaaga ccgaatgcca gacctgcgtg
     2701 cggcggagta ccgtatttca gggcgtcgag caggaagccg aatttctcgc gctgttcctc
     2761 ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat ggatacgtac
     2821 tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg ccaccgcatt
     2881 ttccggtgca gctttcagtt ctgcagcagt catgtctttc ggtgaggtga acggatggtg
     2941 cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga taacccacag
     3001 cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca ggcgcagtgc
     3061 gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca tatcgccatc
     3121 ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga acttcgctac
     3181 cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt aagccagacc
     3241 tttcgcgccg tagattttaa cgaagttacc gtattcgtcg atctgcttac gggtcagcga
     3301 tgcgccaccc ggaacacgta gagcggcaac acggcctttc ggatcgttcg ccggccccgc
     3361 aaatactgca aactcaacag atttcagcag atcggcaacg tcggtcagtt ccatcgggtt
     3421 acgcagatcc ggtttatcag aaccataacg gcgttctgct tccgcaaagg tcattaccgg
     3481 gaaatcgccc agatccacgc ccttcacttc cagccacaaa tgacgcacca gcgcttccat
     3541 cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct gagtaaattc
     3601 aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct gatagtaacg
     3661 gtcaaaaccg gacatcatca gcagctgttt gaacaactgt ggggattgcg gcagtgcgta
     3721 gaatttacct ttgtgcacac gagaaggcac caggtagtca cgcgcgcctt ccggcgtggc
     3781 tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa aacggcgcac
     3841 caggctggtg attttagcgc gggttttcag gcgctgagcc atttccggac gacgcaggtc
     3901 gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt caagcggcag
     3961 aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcgattt cgccggtcgc
     4021 catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtga cctgaatgca
     4081 gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg gatcgaaaaa
     4141 tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac caagatcacg
     4201 acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca aacggagctg
     4261 tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg atgccccctg
     4321 ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga aaaaaggcgg
     4381 ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgga acgcctcgtt
     4441 accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa ggtgttgccc
     4501 gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca ttaaggtgtg
     4561 aggcacgatt ttgttttttc aggagtcatt atgcttgaac ttaatgctaa aaccactgcg
     4621 ctggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc acatactgcc
     4681 gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag cggtcagccc
     4741 gtgtttctgg tgcgcgttgg ctggtctgcc gattacgccg aagcattaaa acagccggtt
     4801 gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta
     4861 ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg
     4921 gatctggagt tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg
     4981 accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg
     5041 attgccgaag acgcctgtag tgccgctagc gccgagcagc acaataacag catcaaccat
     5101 atctacccac gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt
     5161 acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg
     5221 aagagtatgc ccgccacttt aactgcgtgg agggcaacac cacgctttac gccctgccga
     5281 aacccgaggt tgtcctgcgc tggcgtgaac agaccacaga tgacttccgc ttctgtttta
     5341 agtttccggc gaccatttcg catcaggcag cattacgaca ttgcgatgat ttagtgactg
     5401 aatttttgac ccgcatgtca ccgttggctc cgcgcattgg gcaatactgg ctgcaactgc
     5461 ctgccacatt cggcccacgg gagctgcctg cgctttggca ttttctcgat tctctccctg
     5521 gcgaatttaa ttatggtgtg gaagtccgcc atccacagtt tttcgccaaa ggggaagagg
     5581 aacaaacgct taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt ttagacagcc
     5641 gcccggttca tgcagcatgt ccacacagtg aagctattcg cgacgctcaa cgaaaaaaac
     5701 ctaaagttcc ggtacatgct gtactgacgg cgacaaatcc gctgatccgt tttatcggta
     5761 gtgatgatat gacgcaaaac cgggaattat ttcaggtctg gttacagaaa ttagcgcagt
     5821 ggcatcagac cactacgcct tatctttttt tacatacgcc agacattgcc caggccccgg
     5881 aactggtaca taccctgtgg gaagacttac gtaaaacgct tccagagatc ggagcagttc
     5941 cggctattcc acagcaatct tctcttttct gaatttgcca cctatcatag acaggtgcca
     6001 tcggccattt taaagggagt ttgtatggta agcgcgctat atgccgtttt aagtgcgttg
     6061 ttattaatga agttctcttt tgatgtcgtt cgcttgcgtt tgcagtaccg cgttgcctat
     6121 ggtgacggtg gttttagcga actgcaaagc gctattcgca ttcatggtaa cgcggtggaa
     6181 tatattccca tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc agaaacctgg
     6241 atggtgcata tttgcggcat cgttttgctt gctggtcgtc tgatgcatta ttacggtttt
     6301 catcaccgtc tgtttcgctg gcgacgttcc ggcatgagcg ccacctggtg tgcgctgttg
     6361 ctgatggtgc tggcgaatct ttggtatatg ccctgggagt tggttttctc cctgcgttag
     6421 cgcacaatac gccactttct ttttcccgga tttttacgtt atgtctcacc gcgacacgct
     6481 attttctgcc cctatcgcca gactgggcga ctggaccttt gatgaacggg tagctgaagt
     6541 cttcccggat atgatccagc gttccgttcc cggctattcc aatattattt ccatgattgg
     6601 tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg gttgttctct
     6661 gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat gataattgca aaattattgc
     6721 catcgacaac tccccggcga tgattgaacg ctgccgtcgt catattgacg cctataaagc
     6781 ccctacgcca gtagacgtca ttgaaggtga tattcgcgat atcgccattg aaaacgcatc
     6841 gatggtggtg ctgaatttta ccctgcaatt cctggaacct tccgagcgcc aggcattact
     6901 ggataaaatt tatcaaggac tgaacccggg cggcgcgctg gtgctttcgg aaaaattcag
     6961 tttcgaagat gccaaagttg gtgaactgtt gttcaacatg caccacgact ttaaacgtgc
     7021 caacggttac agcgaactgg agatcagcca gaagcgcagc atgctggaaa acgtgatgct
     7081 gaccgattcc gtggaaaccc ataaagcacg cctgcataaa gccggttttg agcatagcga
     7141 gctgtggttc cagtgcttta actttggttc actggtggca ttaaaagcag aggacgctgc
     7201 atgatcgact ttggtaactt ttattctctg attgccaaaa atcatctttc acactggctc
     7261 gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg gctgtttaag
     7321 cagtggtcca acgcggtgga atttctgcct gaaattaaac cgtatcgtct ggatttattg
     7381 catagcgtaa ccgccgaaag cgaagagcca ctgagcaccg ggcaaattaa acgcattgaa
     7441 acgcttatgc ggaacctgat gccatggcgc aaagggccgt tctcactgta tggcgtcaac
     7501 atcgataccg aatggcgttc cgactggaaa tgggatcgtg ttctgcccca tctttctgat
     7561 ttaaccggac gcaccattct ggatgtcggc tgcggcagcg gttatcacat gtggcgcatg
     7621 attggcgcag gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt tctgtgccag
     7681 tttgaagcgg tgcgtaaact actgggtaac gatcagcgcg cacatttgtt accgttaggt
     7741 attgaacagc ttccggcact gaaagccttt gataccgtct tttcgatggg cgtgctttac
     7801 catcgccgtt cgccgctgga gcatctctgg cagttgaaag atcaactggt gaatgagggt
     7861 gaactggtgc tggaaacgct ggttattgat ggcgatgaga acacggtgct ggtaccaggc
     7921 gatcgttacg cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc gctgaaaaac
     7981 tggctgaaga agtgtggttt tgtagatatc cgcgttgtgg atgtgtgcgt taccactact
     8041 gaagagcagc gacgcactga atggatggtt accgaatctc tgtccgattt cctcgaccca
     8101 catgatccga gtaagacagt tgaaggttat cctgcgccta aacgcgcggt gctgattgcg
     8161 cgcaagccgt aaaggtctgg cattactgcc ggatgcggct tgaatgcctt atccggccta
     8221 caaacccttg ctaattcaat ctattgcagg gactatgtag gcctgataag catagcgcat
     8281 caggcagttt tacgtttgca taacctcagc gcccgtttcc gggcgctatt cacttcttac
     8341 gcctgaacag caggcttatc aaatgccgtt aacttcggtg cgttacccgt atatttttcg
     8401 atatacacca gcgcagagtt accggcacag gcatttgcca gctgcgagct ggggatatcc
     8461 gccgttaaca cgttcgcact gccgttttta cacaagccgt tttccagatc tggccatgca
     8521 ccttcatgca cgcataccac gccttttttg atcccgtcag tcaccaccgc gcctgtcaaa
     8581 atctgaccac gtttgttcca gacacgcacc agatcgccat tcgcaatacc aaatcgagca
     8641 gcatcttcgg tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt
     8701 tccgcatagt taagctgact atgtaaacgg tgtgccggat gtgcggtcag aagctgcaac
     8761 tgcttctcgt cggcggtacc cttccactca tcaggcgcaa gccaggttgg atgtgccggg
     8821 caatccttat agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc
     8881 gtacccagcg catttttcac cggatcggcg cggaaatcag cataacgaac gtactgttcg
     8941 ttcttctcgc tgcggcgcat ttcgatcagt ttattttgct gccagaaggc attaaacatc
     9001 ggcatagtca cgcgttgcgc acgagcacct ttctgagcgg catcatagaa aaatttcagc
     9061 cacgccattt catctttacc ttcggtatag atctcttttc cgccaggttt cagtaattca
     9121 gcaagatcgg caaatacgtc aaaatcgtta cgcgcttcaa attgcggagc aacagcctgc
     9181 ttcatcggca caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc attgcgctca
     9241 aacgatgtgg tgatcggtaa tacgatatct gcatgtttag cagctgcggt ccagtagcat
     9301 tcagaaacga cgatcatctc cggtttctgc catgctttga tcagacgatt agtatcctgg
     9361 tgatgggtaa agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt
     9421 tctttaccgt tatgctgata tttgccacca ggattttcca gcgcatcgac gatacgggca
     9481 acaggaatcg ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc
     9541 gcagacattt ccggcagcac gccgccaaca cgcgtcggat tgccaccgtt ggagtaatga
     9601 taagagaaac caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc
     9661 accagcatcc agtgtttctg ttcaccatat tgctggcgct gaattcccca gccagccatc
     9721 agcattgtgc gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg
     9781 ccagtaattt ccgccgccca ggccgcgctc ttcggagtat tatcgctctt acctgtcaga
     9841 tactcttcaa actgcggata cccggtggtg tatttttcaa ggaacacttt atcgtgttta
     9901 ccttgtgtca tcagggtatg cgcaatccct aacatcagtg ccacgtcggt gcccatattc
     9961 ggcgcgatcc aggtggcatt atcaccaaag aactcgatgg tttcggagcg gataggatca
    10021 atggcaatca ctggtttgcc agattttttc agctgatgga agtattccag cccttgctca
    10081 tcggtactgc tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca
    10141 gcctggctgt tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa
    10201 ccgaccacat gcggcatgat cacctgtgct gccccggttg aataatcgcc gctatgcccg
    10261 gaataaccgc ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg
    10321 ccagaagagc gccagccgta tgatccggca aaaatagcag atggtccgtt agctttgcga
    10381 atgcgatcgt gttgctcatg aatcagtttt aatgcttgct cccagctcac ctgtacataa
    10441 gtatcttcgc cacgaccttt cgccggttgc agtggattat cgagatagct ttttcttacc
    10501 atcggatgct gaatacgtgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag
    10561 ttcggtatgg ttttcgccag cgcgcctgtt gaagaaacaa tcttgccgtc cttcacttct
    10621 acgttcatcg ctccccaacg tcccgcggtg aggattttac cgcccttctc ttctgcccat
    10681 gcggggagcg gtgctgccga tgtcaccacc agcgctccag cggcaatacc gctgtgttta
    10741 ataaattcac gtcttgttaa tgtcataact tcctccctga tcaacgagga tcactgtttc
    10801 tcggtaatat ctttggcgtt gtactggaaa taccgcgtta aaatgtccag ttcgttttcg
    10861 ctcatgctgg ttcgcgcacc cattcctttg gcaatggacg gccatgcatt gacggtgtaa
    10921 tggtcagcgg caataggcgc atggcaacca gcgcaatagg tatcgtcaag tttttcagcg
    10981 tattgccata gcggtttacg gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac
    11041 gcctgacgcc attgattgcc gtattcgtca gcctgccatt ccccgtttac cgtgagcgcc
    11101 ttgatacctt cttcacttaa tgtggctagc gccagccgtt gacctgccgc caggtagagc
    11161 gtgttttcac tgccctgcat ttgataaccc tgcaacagaa cgatcggctg tttgccactg
    11221 gcatcaacga cggtgagatc ggtaccagga ttcacggtag ccagctcgcc tatgcgagaa
    11281 gttttgaaag gataaatatg tgcgccatta gtcactgaag tagcggcctg actttccagc
    11341 tcatgcgccg cgttgtcatc catttttatt tctggcggaa aatgggcaat gcctttatga
    11401 caatcgatac aggtttcgcc gcccttttgt gctttgttat gcattttctg cgcagattca
    11461 ctttgcgagg caatatccat ggcatcaaaa gaatggcaac tacggcacgt tgcagagtca
    11521 gttgctttta attctttcca tactgtttcg gccatttcct ggcgatgagt ttcgaactta
    11581 tcgtcactgt ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat
    11641 ttagcaaata aataatccat ccctgacttt ggaatatggc aatctgcaca ttccgcacgt
    11701 atccctttct ggttcgaaaa gtggacagtt ccctgatatt cctcaaaagg tttactcatc
    11761 gagtggcagg aaaggcaaaa tgctgtatcc gacgttttat gtaaggcttt ttgcgccagc
    11821 aataacccgc cgccaccaac cacaaccgct atcagcaaaa acaataaccc aatgcgtttt
    11881 ttccctcgca tagattcctc taaataaatt ccagaacacc gaaatcattg tataattaaa
    11941 tacacaacga attaaccgct tgcataactc ggcacaacac tgcctgaaat aatcgataaa
    12001 gaatatgatt tattacaatg taatcattaa ttgctaagga ataacccagt tgctatttag
    12061 aatatatatt tatcagtttt agcaatttaa atcccataat caatgtgaat ataaaaaata
    12121 ttattagcac aacgctatgt tcactgtcac ccgctcaaca gatcacggtt atcgttcgtt
    12181 ttttatactg ctcagggata aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag
    12241 caagcatcat attgggcgac atgatgcaac ggtaaaaatc atttggcctg atggcgttca
    12301 atgattcctt tcatttcagc aaccgccgcc ccgtctacga tataacgcga atactcgtcc
    12361 gcgtgttcat ctgatgacat ggacaatcct tgattacgat aacgcatcgg tgaggcttgc
    12421 cacgctcccg cggagctatg gacttccagc actccggcat cgaggaagtg gtgcaggttt
    12481 tctgcacgga ctcctgctcc ggccataatg attggagcat cacgatgggc aataagttcc
    12541 ataatttttg ataaaccttg cagtgcgtct gatttttgcc ctgatgtcag tacccgggca
    12601 atgcccaatt ccgcaagatt attgagtgta tataaagggt tagcgcacat atcgaaggcg
    12661 cgatgaaaag tcactgccag cggaccggca gcagccatta ttttttccat tcgtggcata
    12721 tcgacattcc cgtcaacatc gagaacgccc gtcaccagtc caggaaaacc taattcgcgg
    12781 accgtgcgca catcctcaag aatggcggca aactcaccgt cgctgtagca aaaatcacca
    12841 ccgcgtgggc gaattatcgg atgcacaggg atcgtcaccc gctggcgcac ggatttcagt
    12901 acccccaacg acggcgttaa gcccccctct tttggggctg cgcataattc aactctgtct
    12961 gcgccgtttt gctgcgccgt tagtgcacat tccatgctgt aacagcaaat ttccagtaat
    13021 gccatttttg ctccttaatt acgctgactg ctcgctggca acgatctctt caatggacca
    13081 cggatgaaac ttaatagttg ttttgccatc ggtgaccgcc agcgtcggat tcggtaatcg
    13141 ctcatgctca ccttttggtg aactggtttt aactgagatc cccggtaggc tgagtccttc
    13201 atcagaaagc aatgccagcg cacgggcatt cagcgtttct ggatcacccg gcagaacgat
    13261 ttcaatatgt tcccaacctt cgtgtgggta acgtttttcc ccgggccacg gtagctccac
    13321 aatagaaaac tgccagtgcg caacctgtac cggttcatgc aatttaaaca gacaaatcgg
    13381 tctgccattg atcatatttt ctgacaaaag ctcgccacac tgttcaaacc cgcgacgcca
    13441 gcgttcagca gtggcgtttt gatggcaacg caaagaaatg tgatcggcag tcagcggagt
    13501 gatattcaac cccagacgga gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc
    13561 cgatgcaata tcctgcagct cgtcgataga ttgccagttc gccataatca ctcttcgtct
    13621 ttcagtaaaa gcgttaattt accctgttgc cctgcgccaa ccaaccgctg atttcacgcc
    13681 gcttctgatg caatagtgaa aacggcaata cgccacgcgc acgttgctga cgaaaacagc
    13741 catttgcagt atactcccgc cctaatttct ttaactggtg cgggcaattt ttgctcgctt
    13801 catcaatgta aggtattccg gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg
    13861 ccatgattgc ggcaggcgcg cctgcggatt gcgaaccgca ggttcgtcag tcagcaaaag
    13921 ttcagttcgg cgactatcag gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg
    13981 caccgcgaca attagcagag caggtgctga ctcatctgga tcttaacggt atcgccagca
    14041 aagttgagat cgccggtcca ggctttatca acattttcct tgatccggca ttcctggctg
    14101 aacatgttca gcaggcgctg gcgtccgatc gtctcggtgt ttctacgcca gaaaaacaga
    14161 ccattgtggt tgactactct gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc
    14221 gctctaccat tattggtgac gcagcagtgc gtactctgga gttcctcggt cacaaagtga
    14281 ttcgcgcaaa ccacgtcggc gactggggca ctcagttcgg tatgctgatt gcatggctgg
    14341 aaaagcagca gcaggaaaac gccggtgaaa tggagctggc tgaccttgaa ggtttctacc
    14401 gcgatgcgaa aaagcattac gacgaagatg aagagttcgc cgagcgtgca cgtaactacg
    14461 tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca
    14521 tcaccatgac gcagaaccag atcacttacg atcgtctcaa cgtgacgctg acccgtgatg
    14581 acgtgatggg cgaaagcctc tacaacccaa tgctgccagg aattgtggcg gatctcaaag
    14641 ccaaaggtct ggcagtagaa agcgaagggg cgaccgtcgt attccttgat gagtttaaaa
    14701 acaaggaagg cgaaccgatg ggcgtgatca ttcagaagaa agatggcggc tatctctaca
    14761 ccaccactga tatcgcctgt gcgaaatatc gttatgaatc actgcatgcc gatcgcgtgc
    14821 tgtattacat cgactcccgt cagcatcaac acctaatgca ggcatgggcg atcgtccgta
    14881 aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg
    14941 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc
    15001 tggatgaagc cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag
    15061 ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc
    15121 tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg gcgtttgagg
    15181 gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg ttccgtaagg
    15241 cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa gatcgtgaag
    15301 cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag
    15361 gcacgccgca tgtgatgtgt gcttacctgt acgatctggc tggtctgttc tctggcttct
    15421 acgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac
    15481 tggcacaact gacagcgaag acgctaaagc tgggtctgga tacgctgggt attgagacag
    15541 tagagcgtat gtaatcgatt tttcgtgaga gtaaagcctg atcagggtta cctgatcagg
    15601 cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt tctgactgtt
    15661 ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt tgaatttaag
    15721 gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt gccaggctta
    15781 cgcagaagat acggttatag taaacgacca tgacatttca gccatcaaag attgttggca
    15841 aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc gacaagaata
    15901 caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt atatagaaca
    15961 agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc ttaaaaaatc
    16021 acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt caaatgagga
    16081 cgttcaggat ttaagtgacc cttattctga atcagaatgg ctctcatgta tgatcataca
    16141 gaccaatacg cgaactcgcc agttgcagct ataccgtaac tctgaagatt tttatccaag
    16201 ccctttgaca agaggataat tcacatcttt ttggcatgtt ttgttgcaag ctattcctga
    16261 taaataattg caacaagaca tcgagccttt ttcactgagt tattaaacat actcgcgagc
    16321 gcgtaatttt tttgtccttc agcgataatt cacaatcact tcattacgtt gtacctttag
    16381 cggtggaatt aaccgcccac cgcctggcac ttcccagata aatcgcaacg gttctgctgc
    16441 cgctataccg gaaaaggcca cggtggttcc gctctgcccc tctaattcga cacaacgaga
    16501 ttgcgaacac aagcgaaccc gcagtcctga aggtgtcggg ccgataagct gataacgcca
    16561 cgctaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg cagacgacat
    16621 cgactcaccg cgatgattta gcgtaattcc tacactactt gcctgccaca tcccctcccc
    16681 ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggttctca ttatttgccg
    16741 ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt cgacaggacc
    16801 actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcgg cgcgtggttc
    16861 accaacaata ctggcggcgc acccagcatc tcctgacggg atagcgcttc ctgagtttgc
    16921 gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag cgcctgtagt
    16981 aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac
    17041 cactgctggg taatcgctcg tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga
    17101 tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc
    17161 ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag cgtgacgacg
    17221 ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc caacagctgt
    17281 tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc
    17341 gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc ctgttctttt
    17401 agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca
    17461 ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca
    17521 ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagctccata
    17581 ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa tttcttgcgg
    17641 atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat cgggatcagt
    17701 cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg
    17761 acaacggtat tattctctgc catttttacc ggtttgggtt cgacaggcgc tttttgttcg
    17821 cgtccgcgta tccaccaggc cagccccagc aatccggcag tgaacagcaa aaataccagg
    17881 ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg
    17941 cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca
    18001 cgcgtaacga tgacccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga
    18061 ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccatgt
    18121 tgcagcacgc cgaccagcaa cccgccgaca acgttaatga ccatgatgag gatcccggcg
    18181 atggcatcgc cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa atcggcttcc
    18241 tgagtcactt cggagcggcg ttttttcgcc tcatcttcac caatcaatcc agcgttaagg
    18301 tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg cgcacccact
    18361 tctgcgatac gcccggcacc tttggtaatg accataaagt tgatgatcac gagaatgaca
    18421 aacactacga taccgatagc gaaattgcca ccaacgagga agtgaccgaa tgcttcgacc
    18481 accttcccag ccgccgccgc gccggtatgc ccttccatca aaatgatacg ggttgaagcc
    18541 acgttaagtg ccagacgcaa caacgtagta aacaacagaa tggtcggaaa cgcagcaaac
    18601 tcaagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa ggcaatattg
    18661 aaggtaaaca acaggtcgag tatgaatgcg ggcagtggca gcaccatcat cgacaagatc
    18721 aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc
    18781 aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa aatccagggc
    18841 ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca gacgccagcg
    18901 tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt gacccgggat
    18961 ttgttgacca atctccgcat gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt
    19021 cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt
    19081 agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg
    19141 ggtcgggtta ttgacaatga catccgcttt cggcacatcg gccatcatcc gacgccgtgc
    19201 agcagctcgc tgcatctgac ggatccgccc tttaacatga gggtcacctt cgctttgttt
    19261 gaactcatca cgaatatcct gccgtgacat acgcagcttt ttcaggtggc tgaatatttg
    19321 gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca gtgcgcatag
    19381 ccctaccaaa tccatcgcat tacccatggc ggtaatcgga gactcggcca tcaagcgcat
    19441 catctgcggc cagtgatgcc agagaaaaaa ccccgtcacg ctgccaacca ggatggtttt
    19501 caaaattgct ttaagcaact ccgcgccagt ctgagccgag aacatccgtt taatgcccgg
    19561 tagcgggttg agtttggaaa acttcggctg caaggatttg ccgctaaata ccagccctcc
    19621 cagcatgacc ggagaaatga gcgccaccag caccacgccg ctaatcagcg gcagcagcgc
    19681 cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg ggtcattgat
    19741 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
    19801 cacaccgcca aaccagataa cactaacgcc caccagcaaa atcagcagtg aggtcagttc
    19861 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
    19921 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagcgt
    19981 gaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
    20041 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
    20101 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
    20161 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacaccgg ctttgctggt
    20221 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
    20281 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
    20341 catccgctta atgacctgcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
    20401 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
    20461 tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
    20521 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
    20581 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
    20641 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
    20701 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
    20761 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
    20821 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
    20881 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
    20941 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
    21001 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
    21061 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
    21121 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
    21181 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
    21241 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
    21301 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
    21361 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
    21421 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
    21481 taccgaaggc cgatgacggt taaccgccgg gccatcgtga attttgattt ggtaatttgc
    21541 gccactacgc gacagctcca tatgccgatc gcccggcgca atataggcat gccccggcaa
    21601 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
    21661 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
    21721 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
    21781 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
    21841 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
    21901 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
    21961 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
    22021 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
    22081 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
    22141 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
    22201 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
    22261 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
    22321 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
    22381 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
    22441 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
    22501 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
    22561 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
    22621 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
    22681 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
    22741 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
    22801 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
    22861 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
    22921 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
    22981 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
    23041 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
    23101 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
    23161 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
    23221 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
    23281 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
    23341 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgtcc
    23401 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
    23461 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
    23521 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
    23581 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
    23641 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
    23701 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
    23761 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
    23821 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
    23881 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
    23941 ttacgcacat cactcaccgt cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa
    24001 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
    24061 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
    24121 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagcgcc
    24181 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
    24241 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg attcctgcac cggcgcagtg
    24301 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgttg
    24361 tgccagcggg caaaactttt ctccgtctct ttttgcaacg ccctgacgtg ctcgttgccc
    24421 gcagtcatcg ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc
    24481 gtcgtcatca acgttttaat gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct
    24541 ttgttcagcg cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt
    24601 tgctgattgc tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa
    24661 gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg
    24721 gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta
    24781 gtaaatactc gtcaaaatgt ttcccagttt ggagcttgtt cagcaattcg cagtcgtggc
    24841 tgtgccggtg gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg
    24901 ctggctgcca gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc
    24961 gcagcggcgg cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc
    25021 atttccgaaa ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat
    25081 gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt gttcattgtt
    25141 tccccggcgc tttcgaccag caccgaaccg gtatcaacgc gtgagacgga gtcttcaatg
    25201 agggctttga tctcttttgc cgcctgggcg ctgcgactgg caagattacg cacttcaccc
    25261 gccaccacgg caaaaccacg gccctgttca cccgcacgcg cggcttcaac cgcggcattc
    25321 agcgcgagga tattagtctg gaaggcaata ccgtcgataa cgctgataat gtcggcaatt
    25381 ttcttcgaac tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg
    25441 ccgtgctggg cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg
    25501 gcgttttgct tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg
    25561 gatgcctgct gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta
    25621 ccggcataga tggcatctga accttcgcgg acatgagtga cggtgtcagt caaagagcgt
    25681 tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg
    25741 gtattcgcca ggttaccacc ggcgatttcg cgaatgtgag caataatttt tgccagcgga
    25801 gtaagcaaca tacggcgaat gccgtaccac gccaccagca gaatcaatac caccaccagc
    25861 gcgataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata
    25921 tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt
    25981 tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc aagataatca
    26041 atcagttcag ttaacgctgt gtgatagttt ttatattttt catcaatatt acgactggta
    26101 gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgagt cgcggcctgg
    26161 gccaatgttt tcctggcgct atcgagcaat tcaactttgg cgttactttg ttgattggag
    26221 gaatccatca tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa
    26281 tcccaggttg acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc
    26341 ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg
    26401 aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac
    26461 cttcctgaaa acaagttaat ctcgttatcg gcaagaaggg gggaaacttt attgctgatg
    26521 ccacccgccg cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat
    26581 tgaggaaatc gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct
    26641 tcgctgttca gcagtttttc gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc
    26701 agtccagtga gatattctgt tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc
    26761 tccgccgtca atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt
    26821 ccgagattca ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac
    26881 ttaattcgta agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata
    26941 aacgctggcg tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc
    27001 aggatatcaa taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac
    27061 ggctcgctgg ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca
    27121 ttcaggcggc ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat
    27181 caacaatcag tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt
    27241 tgcgatagtt actttcaagg tttttaacca caacctggtg ctgaccaatt aattgatcca
    27301 ccagcaaggc gtagcggcga ccgccacttt gtaagatcac cacaattccc tgggtggctt
    27361 cggttttcgc gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac
    27421 cccgcacttc cagcacccgc tcgccgccgg ccagtggatg cagatcggct tcacggggtt
    27481 gcagtgattc cataacagca ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg
    27541 acatgccgtc gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac
    27601 cctgcttcga ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt
    27661 ccatgccgac gccgcgcccg gagacgtcgg tgacctgctc tgccgtggag aagcctggtg
    27721 caaatatcag catcgcgact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg
    27781 aggccgcttt tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa
    27841 tgcaaatgtt gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac
    27901 ctgcggcgag ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt
    27961 gggtcagcgg gtcgataatg cgttctatca ggcttttgtc gagttcagta gaactgccca
    28021 ccagcgtcag ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat
    28081 agcgactaaa aacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat
    28141 cacgggcgtt acgttgtaac tgccccatgc tggttatcaa atcaccatga ttaaccgggt
    28201 ccagttcgct ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt
    28261 taattaattg atcaaccttt tctaccgcta cacggatgct ggtggattca ttgctgcgcg
    28321 tcgttttttc ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcactg
    28381 gtggggtgga tatttttggc gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa
    28441 tcacaaaaca gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg
    28501 agagcgaatc cgcccctttc accacgtcag ttaacgttgt cagatgtccc agttcttctt
    28561 ccagcaggtc gacttccccg gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac
    28621 tgcgactctg ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg
    28681 atggcgtttc gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga
    28741 agctggcggc atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt
    28801 ccttcgtttc caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg
    28861 cttcatcgag caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag
    28921 ttcctgcccc tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat
    28981 ctggcgcttc cggctgcaaa accagcagat gctgctccat gtcagccaac agttcgtccg
    29041 cttcatcaaa aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt
    29101 tcggctgatg gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttccagggcg
    29161 cttactggct cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg
    29221 ttcagtacca gcaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag
    29281 cgcatcgttg ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc
    29341 atcagttcgc ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctatatcct
    29401 ttctcaccgc tggcgtaggg gaaatcatcg gtatgacctg aaaggctaat acggttggga
    29461 ataccgttca gtacaggcgc aatggcacgc agaatgtcgc gcatataggg ttcgacatcg
    29521 gcactgccgg ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct
    29581 tcctggacca gatcgatttt gagatgggga cgtaacgccc gcagtttcgg atcggactct
    29641 atcaactggt cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc
    29701 agctcttcga tgttcggctg cttattcact tccccctggc tttgggtgta atcatcacca
    29761 ccgccgggaa ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc
    29821 agtggagtcc ggaagtactc cgcaatctga atcagctctt ttgggctgga gatggagatc
    29881 agccacatca ccagaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc
    29941 cacgatccat gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga
    30001 tgcgcttgat tcttcatgct tcctcggttg tcatctgttg ttgcggattt ttcaccgcac
    30061 gcacatgctc ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac
    30121 caaactcaac ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga
    30181 cgcactgcat cattttgctg gtttcggcgc ttttctgacg taaaacactc gctaatgggg
    30241 aaataaatcc gtaagccaat aaaatgccga ggaaagtccc caccatcgca tgtgcgataa
    30301 gcgcacccag ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta
    30361 cagccgcgac aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg
    30421 ggacttctgc ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga
    30481 aggtgttcat atgaccgctg ataatcagac gcagataatc gacgataaaa tcaagcatga
    30541 cgctatccgc gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa
    30601 tatcacgttc cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca
    30661 gagccagcag atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca
    30721 gcgccttcag cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc
    30781 ctgcaccggc aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg
    30841 tcatcaaata accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca
    30901 cgacatcatc cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc
    30961 gattggggca aaaaaaagca gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc
    31021 gtatcagtta aacagcctgt actctctgtt catccagcag ttgtgggata atatcggcag
    31081 gattctggga aagtttacgt ctttttactg cccgggatgg cggttgacat aagctgcagg
    31141 caaagctgcc aacaggctgg tgagcgtggg taataaaatt gccgccgcag cagttgcagc
    31201 tggaaagttg cagtaatcca ctttcaacaa accgcaccaa tgtccaggca cgggttaatg
    31261 ccagcagtgg tccttcttct gcttgtgggc actgttcaag gtataaacgg taggctttga
    31321 tcaccgcatc gacgccatta cacaaaccgg ttttcagtaa aaactgccat gcattacaga
    31381 acatcgaagc atgaacgttt tgttcccagg tcataaacca gtcggttgag aatggcagca
    31441 tgcctttcgg cggtgggctt ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac
    31501 ttaactgtgt ttcgctttcc agcatctgca aacgagcgcc cagggtgatc aattccattg
    31561 ccagctgaat atcccgcgct tcctgaacaa tgcttttttc actcatgatc aggccctttt
    31621 cttgcgcagc gcttcttcag gctgattaac atcattcagc aagcgtgttg agagcatgat
    31681 gccggtatga atttgctgga gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt
    31741 ctggtggctg tcaaaacgga agtgacaaac cagttgattg gtttctgcca gcttaaccat
    31801 ttgcggaaga gtcagtgccg ctaacgttgt cgccatttct tcatttatgc cgagacgaaa
    31861 catagcggac gctttgtcct gaacaatcaa acgctgtgca agtagtaaat atgacaagtt
    31921 gatgtcataa atgtgtttca gcaactcgga ggtatgcatt attcccaccc agaataacca
    31981 actttatttt tatgcggttt caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa
    32041 ataattagca ttagaatagt tgcgataagc tgcaataagc agaaccacct ttttggttta
    32101 atatgtcctt acaaatagaa atgggtcttt acacttatct aagatttttc ctaaatcgac
    32161 gcaactgtac tcgtcactac acgcacatac aacggagggg ggctgcgatt ttcaataatg
    32221 tgtgatgcag atcacacaaa acactcaatt acttaacata aatgtataag tcatactttt
    32281 gttttgggtg tatttcaatc tgttaaaaag tttttcgcta cgctagcaag caaaaatgaa
    32341 acaggaataa tcgaaatggg atgttgcgca cagttaaaat aactcaccgt aaataatcat
    32401 ctgctataaa taatcacttt catgcaatac cagataagct at
//
LOCUS       DAZKGS010000038        32324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKGS010000038 DAZKGS010000000
VERSION     DAZKGS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(546..635)
                     /locus_tag="ACHOQY_001761"
     tRNA            complement(546..635)
                     /locus_tag="ACHOQY_001761"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Ser,seq:cga)
     gene            729..1526
                     /gene="mtfA"
                     /locus_tag="ACHOQY_001762"
     CDS             729..1526
                     /gene="mtfA"
                     /locus_tag="ACHOQY_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9677882.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1627..1702
                     /locus_tag="ACHOQY_001763"
     tRNA            1627..1702
                     /locus_tag="ACHOQY_001763"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1660..1662,aa:Asn,seq:gtt)
     gene            1864..2779
                     /locus_tag="ACHOQY_001764"
                     /pseudo
     CDS             1864..2779
                     /locus_tag="ACHOQY_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2973..4277)
                     /gene="ybtS"
                     /locus_tag="ACHOQY_001765"
     CDS             complement(2973..4277)
                     /gene="ybtS"
                     /locus_tag="ACHOQY_001765"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFP9677883.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(4305..5585)
                     /gene="ybtX"
                     /locus_tag="ACHOQY_001766"
     CDS             complement(4305..5585)
                     /gene="ybtX"
                     /locus_tag="ACHOQY_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFP9677884.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(5578..7380)
                     /gene="ybtQ"
                     /locus_tag="ACHOQY_001767"
     CDS             complement(5578..7380)
                     /gene="ybtQ"
                     /locus_tag="ACHOQY_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFP9677885.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(7367..9079)
                     /gene="ybtP"
                     /locus_tag="ACHOQY_001768"
     CDS             complement(7367..9079)
                     /gene="ybtP"
                     /locus_tag="ACHOQY_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFP9677886.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            9336..10295
                     /gene="ybtA"
                     /locus_tag="ACHOQY_001769"
     CDS             9336..10295
                     /gene="ybtA"
                     /locus_tag="ACHOQY_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFP9677887.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            10486..16593
                     /gene="irp2"
                     /locus_tag="ACHOQY_001770"
     CDS             10486..16593
                     /gene="irp2"
                     /locus_tag="ACHOQY_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFP9677888.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            16681..26163
                     /gene="irp1"
                     /locus_tag="ACHOQY_001771"
     CDS             16681..26163
                     /gene="irp1"
                     /locus_tag="ACHOQY_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFP9677889.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGSVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWHTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELASYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     SLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLASHVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALSCGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAVS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRKTSAWHDAWDYWQEKLPQLPLAPELPMVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFTAHP
                     EAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGFIEG
                     LSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPGVNR
                     PDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNSALEK
                     QVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHSTLSD
                     FCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQAKS
                     PQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRMVLI
                     DPVCRQDFCCENRAALLRLLAEGETPLALPEHFDQQTPDSQLADFISLAKTAGMVSQN
                     LTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETELQGWINN
                     ADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26160..27260
                     /gene="ybtU"
                     /locus_tag="ACHOQY_001772"
     CDS             26160..27260
                     /gene="ybtU"
                     /locus_tag="ACHOQY_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFP9677890.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            27257..28060
                     /gene="ybtT"
                     /locus_tag="ACHOQY_001773"
     CDS             27257..28060
                     /gene="ybtT"
                     /locus_tag="ACHOQY_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFP9677891.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            28064..29629
                     /gene="ybtE"
                     /locus_tag="ACHOQY_001774"
     CDS             28064..29629
                     /gene="ybtE"
                     /locus_tag="ACHOQY_001774"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFP9677892.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            29760..31781
                     /gene="fyuA"
                     /locus_tag="ACHOQY_001775"
     CDS             29760..31781
                     /gene="fyuA"
                     /locus_tag="ACHOQY_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFP9677893.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         7052 a         9667 c         8855 g         6750 t
ORIGIN      
        1 atgtctgtag aaaaaaatgc gaaaaaggcg aacaataaat acacaactca gccatacaga
       61 ctggcagcgc aaatgtttcc aggagcaagt catctgtcat ggtaaagcta accagaaata
      121 taggttaatt ttggtgttta aacctttttt acttgttagt atgatacaaa ggctttcaaa
      181 aaagctggga aattcaacaa ctcttggaaa ttatggactt tgtcccgcgg ctttaatctg
      241 acggccgcgt tccttttttg actcaattat tgaccccttc tctacgcaac ttcagttccc
      301 gccagcaact ttgcggcagt ttcataggat caatgtctaa aagaataatg gtgaccgata
      361 agaaaacgac tgaataactg tagattttcg ctcgaaacct tcctgtcagc tccatagcga
      421 atcaagtgct gaatgtcaca gtatcgaaca gaaaacagtg acgatctaac ccttcaagaa
      481 tattctacga ttgttctgtt taggaaaagc aaggcgggaa gtcaggagat aagtcattga
      541 taaagtggcg gagagagggg gatttgaacc cccggtagag ttgcccctac tccggttttc
      601 gagaccggtc cgttcagccg ctccggcatc tctccgttca gatggttgcc atgatgccag
      661 gaaatttggc attttaacag tccctgtccg tgcaattttg ttcaagtgac gagtttgcga
      721 gcaaaacgat gattaagtgg ccctggaaag tacaagaatc agcacatcaa actgcccttc
      781 cctggcagga agcactatcg atcccccttt taacgggtct gacggaacag gaacaaagca
      841 aattagtcac tcttgccgaa cgttttttac agcaaaagcg gcttgttcct ttacagggct
      901 ttgaactgga ttcattaaga agctgccgga tagcacttct attttgccta cccgttctgg
      961 agttaggact ggaatggctg gatggttttc atgaagtctt aatttatcct gcgccatttg
     1021 tggtcgatga tgaatgggaa gacgatatcg gtctggtgca taaccaacgt attgttcagt
     1081 caggtcagag ctggcagcaa gggcctatcg ttttgaactg gttggatata caagattctt
     1141 ttgatgcatc tggttttaac ctgattattc atgaagtcgc tcataagctg gacacccgta
     1201 acggcgatcg cgccagcgga gttcccttta tttcgttgcg tgaggttgct ggctgggaac
     1261 acgatcttca tgctgcaatg aacaacattc aggaagaaat cgaattagtt ggtgagaatg
     1321 cagcgagcat tgatgcttat gctgccagtg atcctgctga atgttttgcc gtactttctg
     1381 aatatttctt tagcgcccca gaactttttg ctcctcgttt cccttcattg tggcaacgtt
     1441 tctgtcaatt ttatcaacaa gatcctttgc agagactgca tcacgctaat gatacagact
     1501 cgttttcggc gacgaatgtt cattaattaa caactttgca gattaattaa ccaattgaaa
     1561 tggcttatga aatttagtgt tgacagacaa ggtaccgcta agtaatatgc gctccgttca
     1621 cacgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt
     1681 tcgagtccag tcagaggagc caattttctg ttttcatgca tccttgcaaa tctttatctg
     1741 atgttgattc aacaggttag tgtgaaaact cttctcggtg cattttgatt ttaccctatg
     1801 catcgggaaa aattggtggt caaatctggg atcaggttag ttcgataatg gagtgacccc
     1861 catatgttcc ttaccgacgc aaaaatccgc accctcaagc cttctgataa accctttaaa
     1921 gtctccgatt ctcacggtct gtatctgctg gtcaagccgg gtggctcccg ccactggtat
     1981 ctcaaagctg gaggttgcgt cccgcacacg gcagcacctg agtaacataa tgcgccatgc
     2041 ggtccatcag gagttaatcg atacgaaccc tgcagcaaac cttggcggcg tgaccacacc
     2101 tcctgtcaga cggcactatc ctgccctgcc gctggagcgg ctgcctgaac tgcttgaacg
     2161 tattggggca tatcatcagg gccgtgaact gacccggcat gccgttctgc tgatgctgca
     2221 tgtgttcatt cgctccagtg aactgcgttt cgcccgctgg tcagagattg atttcacaaa
     2281 ccgagtctgg acgatacccg cgacgcgaga acccattatt ggcgtgcgtt attccggccg
     2341 cggggcaaaa atgcgaatgc cgcatatcgt ccccctctca gaacagtcca tcgccattct
     2401 gaaacagatt aaggatatca ccggtaataa tgaactgatc ttccccggcg accataaccc
     2461 gtataagcca atgtgtgaaa acacggtcaa taaggcactg cgggtgatgg gttacgacac
     2521 gaaaaaggat atctgcggtc acggcttccg ggcaatggca tgcagtgcgc tgatggaatc
     2581 gggtttatgg gcaaaggacg cagtagaacg ccagatgagt catcaggagc gcaataccgt
     2641 gcgcatggct tatattcata aggcagagca cctagaagcc cgcaaagcga tgatgcagtg
     2701 gtggtcggat tatctggaag catgccgaga atcttatgcg ccgccttata caattggtaa
     2761 aaataagttt atcccatagt atactaacga atccctcact ctggcaaatt accaccacct
     2821 ccacaggagg tggttctata tagactttta tcaacttcat gacgaaaaac acctatttta
     2881 tcccaaaaga agacaataga gtcacagagg aatcaaagag cgactcaatt gtctttggac
     2941 agtctggttg tgaggttaga aaacagttac tcctacacca ttaaataggg cgcaatgctc
     3001 gctaatttct cccgggtttc tgtcagttcg cgttccggcg tagattgcgc gatgattccc
     3061 gcccccgcct gtatccagca gcgctggcta tcctgaaata cggaacgcag aactagcgcc
     3121 gcatcaaagc gcgtatcgtc cagcagcagg attgcgccgg aataaagctc tcgcggcgtc
     3181 ttctcaattt gcataatcgc gttcagagca gcatttttag ggatacctga ggcggtaatc
     3241 gacggaaaca gcacggtaaa cgcatcccag gcatccttgt tttccgaaag ctgaccgctc
     3301 acgccagacc ccagatgctg aacgctgccg cgctggcgaa ccgacattaa atcttcaacc
     3361 accacgctgc ccggctggca aacagcctcc agttcagcaa tagcttcttt gacagaaagg
     3421 atatgctcaa gcacctcttt actgtcgtgc agcagttctg cctctttcgc cttattatgc
     3481 tccgggtttc ccatgcgatc gcgggtgccc gcaagcggtt cagtgaccac tttattgccc
     3541 atcactgaca tcaccagttc cgggctaaag cccagcgctt cgcgtccttc ctgacggaac
     3601 ataaacgagc gcacaggcgt gtttgcctgc cgcccgtata acagcgtggc gggcatatca
     3661 atccgcgatg gcaggggaat ggcgcgcgag acaatcactt tgacatactc gccacggcgg
     3721 atctccgcaa cggcgcgcgc aacctgttgt ttatatgcct caccattgag ggcggtatcc
     3781 accgccagtg gtgcgttctg cgtcgttgta ccggcctctt ttacccactc gcacaaacgt
     3841 cggcacccgt cggcggagtc cgcataaacg gtgacatttc ccttttcaaa aataagttct
     3901 tcacggggaa cggttaacgt cagcagcggc cactccccgg cgttaaaggc aatcccccgc
     3961 gcatgcgccg caaagttgaa cccaacctgt ccatataccc gacgtccttt cacctgagga
     4021 tgcgccataa aacgacgcgc gcagtcggca atggaatcca ccgcccattt ttgctccccg
     4081 gcatcatcaa taaatgtcgc ctgcgttccg tcggcattaa tgtgcagccg tgcctggcac
     4141 cctttgccta aataccaaca gggttgacgc tcatagacat aacactcttc ttctgcgaac
     4201 tggcgtaaaa cgccagaaat cgtcggtagc cattgttcct ctggtaacgc cagatgtaga
     4261 aattcactga ttttcatccc ttacctctct gtgttattcg cggatcaatc aattgcgctt
     4321 gtcatcagtt ctcttgccga atgcagccgg atataagcaa cccacgccgc cagcaacgtg
     4381 aatgccccgg caaacaggaa gcaggccgca tagccaaaat gttgagcaac aacgccaccg
     4441 ataacgcctg ccagaatggc gattgccgca tcagtacatt gaaagagggt gaagtcgaca
     4501 ccggcctgca aaggcgatga aagcgacatc agcgtggcgt aaagacagac cagcgcgcag
     4561 gccagcgaaa tggactggac aatgaccaga cactggagaa tctgcagcag cagatgaccc
     4621 ggcgccatca tgagcgtcat caccaccgcc agcagcgcga tcccctggac gccataagcc
     4681 gtcaacagcg ctctgccggg tgaggtgtat ttcatcagca atccgccagc cagcgttcct
     4741 gctatgcccg ctgcaatatt gccgccgctg aacaacgcgc ccaatgcgga catgctcaac
     4801 ccatgatcca acaacagcgg tgccagaaga ggcagcacaa accgcatgcc tgaattcagc
     4861 atcaatacca gcagtaagcc caggcgcgcc tgcttcctcc ttagcgcata acctaacgcc
     4921 ggaacatgca ggatagtcgc tgttcgcgtc ggctccgtaa tgcgccacag cgggagtgac
     4981 agcgccataa tcagcaccgc catcatcagc atggcgacag gccagccgga tgccgccgac
     5041 agcatcatga acaccccgcc gccgcacatc attcccagat agctgccgcc gacctgcacg
     5101 ctgtttcccc agccgtaacc cgtgcgggtc agttgatcga cacaaaagcc gtcgctggcg
     5161 atatcaatgg tggaggcgac cgtgcctgcc accataaaca gaacaaataa cgcgacgatt
     5221 tgcgtatcgg ggaatccccc ttctcgccta aaccagccaa tcgctgcggc aatagtcagg
     5281 atcgccgcta gcgcgacctg accacgcagg attaacatgc gggaacggcg ttcctggcta
     5341 ccgggcggaa gccgccagcg ctcgatccac ggcgcccaaa taaacttcag cgcccagggc
     5401 agcataaaca gggttgtcgc accggcaagc gccagcgaac cgccagcagc gcgcaccagc
     5461 gcgggtaagg actgcataga cagcgccgat accagactct gcgtcacata gacgccggca
     5521 atagcaaaaa tcacccgccc ggtcgtcaac gtcagcggtt tcacattcga ctgaacatca
     5581 ctcatgcacc cactctccag acgcggaaac cccgtcgtcg cgccacacgc gcgcggccat
     5641 ttgcgcctgc cacagcgcct gataacgccc atgatgtgag agcaattgcg catgagtacc
     5701 ctgctcaacc acctgtccct cttccatcac cagaatgttt ccggccccgg cgatggtgga
     5761 taaacggtga gcgatgataa tcaccgtccg gttgtaaacc aggttatcga tcgctttttg
     5821 caccgccagc tcgctttcaa tatccagcgc ggcagtcggt tcatcgagaa tgacgactgg
     5881 cgcgtttttc aataacgctc tggcaatgga aatccgctgg cgctcgccgc ccgatagctg
     5941 gccgcccatc tctcccattg gcgtcagcca gccttgcgga agacgggaaa taaactcaag
     6001 gcactgcgcc gcgcgggcgg cctcttctac ctcctgccgc gtcgcctgtg ggcacgcgat
     6061 acggatattc gccagcagcg tgtcatcaaa cagccagaca tcctggaaaa caacagaaat
     6121 caggctattg agctgttccg gcgtcaggcg gcgaatatcg acgccgccaa tagaaatctg
     6181 cccttgctgc ggatcggcat agcgcattaa cagtttggtg acggtagttt tgcctgcgcc
     6241 ggaggcaccc accagcgcgc tcatactggc tgccgggaac gtcaaagaaa catgattaag
     6301 cgcgtggccg tcgccttctt cgtagcgata gctgacgttg tcaaagcgga tatcgtaacg
     6361 ttccggcatc tcgctttgct ctgcgacggg aagcggtgct atcgccataa actgctcaat
     6421 ccgttgcagg gcgctggcga tcagttccac aaccgaggtg tagctgataa acatcgccat
     6481 cggttctgcg aagcgcataa tcatcgcgac ggcggcaatc aaaaaggcga ggttcagagt
     6541 gcccgtcact acccagacga tcccggataa caccaccacc tgtaggccca actccacgac
     6601 gctggcgatc agcatcgtcg cgccagcgcc ctgacggtga gtgcgggtct gtaagttttc
     6661 cagcgcattg aaatgcgcca gcagcgcccg gcttttatcg gcatcgctgc cgcaggtgcg
     6721 caataccatc atcccctgag caaattcaac gatatcgccg ctcagacgct ggtgcgcttc
     6781 ccccagcgtc tgcatttgtc gccgcatcgc cgggcggcgc cagtaataaa acggcaccag
     6841 cagagggaag atcagcaaca tcaccagccc cagccgccag tctatccaca atgtcgccag
     6901 cgacgccgtc agcggcgtga caatggtgag cagcaaaata ttggctatcg caataacata
     6961 attgaggttt tcatccacgc tgcccagcag caaggcgttc atttcacccg ccctgccgcg
     7021 ctggagcttc tccagcggca cgcggcgtaa ctgctcgcca agtcgcaggc gcaactcatg
     7081 ggtagcctgc gccagatgac cacggtattc aaagcccagg ccataccagc gtagcaccag
     7141 cgtcacaatt gcggcgacgc tgaaggccat agcccagtta agaagttgcg gcgcgtctcc
     7201 ccgtaacagc gcatcaatga tcggataaag acaggcaaag gcgatcccct gcatgaatgc
     7261 cgccagcaac agcgccagca tactgcgtcg cagcatcctg gcctgactgc caacgctgga
     7321 gataagctgg cgccagatga cgcgccaggc caggttatcc gcaggattat tgtctttcat
     7381 tttccaccac ctcttcctgt gttccacccg gcacccaatg ctgcgcctgc tgatagtgtt
     7441 gccagagccg ttgatacagg ccgccctgcg ccaacagttg cgtatggttc cccatttccc
     7501 tgagctgtcc gtcggaaaac agcaatatca catcggcctg agtcaccatt gagagacgat
     7561 gcgcgaccat gatgaccgtc cggccacgca tggccgccgc gagcgccttg ataagcgccg
     7621 cttcgttttc cgggtcagca aacgccgtcg cctcatcgag caccaggatg gggcgatcct
     7681 gcaaaagcgc ccgggcgata gtaatgcgct gccgctggcc gccggagaga aataccccac
     7741 gttccccgac tcgagtgttg taaccttctg gcagagcgct aataaaatca tgggcctgcg
     7801 ccactctggc cgccgctatt accgcctcca gcggcgtatc cggcgcgccc agacgaatgt
     7861 tattggctat cgtgtcgaca aaaaggaagt tgtcctgaaa cacaaacgag agttgcttca
     7921 tcagggtgtc cgtctgcata tcacgcagat ccacgccgcc aatacggata tggcctttgt
     7981 ccgggtcggc gtaacgcagc agcaagcgcg ccacggtgct ttttccggcg ccgcttggcc
     8041 cgaccagcgc cacaatttgc ccggcaggca catgaaagct cacctcctgc aacgcggcgc
     8101 cagtacgcgc ttgcggataa tgaaagctca cctgctcaaa ggtaatgctc gcctcttgcg
     8161 gctgctgctc agactgcggc agcgataact ccggcatcgc gagaacctga taaatacgct
     8221 gaatgcttaa acgcgtttgc gcgaccaggt tattgagcat catcattggc attacggctt
     8281 cggccattcc gctgcccagc agtaaaacgg ccacccacgc gataaaatcg aaactgccat
     8341 agtgcaacag gccgtatccc gaccagatca ggacaaacag ggtcgggaga ggattcagaa
     8401 tcgagaagga aaaacgcgct gaaaaaccgg ctttgcgata ccaggtttgg agcacatcga
     8461 cccactcttc aagggcgcgt tgatagcgca aaaaactggt gctgccgcta tcaaacgttc
     8521 tcaccacggg catggcctgc acgaactcaa taaccgccgc gctaacctgc tcgcgcgcct
     8581 tatgataacg ctgagccata tcttccgaac gctggcgggc gagcacgaga acaaccgatc
     8641 caaacgccag tacccccagc gtcgcgattg ccagccgcca gtccagccag aacaagataa
     8701 cgattgtcgc cagcggcatg atgatcgcgc gggcatagag cggcgtgctg tcggccacaa
     8761 aaatatgtaa cgacttcacg tcatccagca tcactttcgc cactgacccg ctgcccattt
     8821 gctgtaacac gccaggcgga agctgcaatg ctttacgggc taacctgctg cgcagaatgt
     8881 tctccagatg aaaagccgcg taatgagagg tgttgaacgc ctgtaagcgc agaacaaaag
     8941 ttaagaccac gcagccgatc acgccaccca gcggccaggc cggaatagcg tcaggcgtcg
     9001 cgcgaatgtc acgcaggctc cacaccagaa aaaggagcgc ccccaggctg gttaatgccg
     9061 ccagccccgc cagcgccata gctaaaataa ccttcctgcg aaccggtgta atcacctgcc
     9121 agagaggaaa tcgggacagc gattcagtat tggatgattg agatgacatg ggagtaactg
     9181 aatttcctga tgcattttta cggacgccaa aaacgacaat tcagggtggc gttctgagaa
     9241 ttaatgtgaa taataaccat tatcaataac tcatctaccc cattcggttc tatttatacc
     9301 cgcattggtc taagccacag ggagataacc aggtcatgac ggagtcaccg caaacgcaat
     9361 ctgaaatctc tattcaccag ttggtggtcg ggaaacctgc aaacgatggc aatattcctg
     9421 ctcagtgtga actgctgcgc tgttcgttgc aggaaggtat ggatattttg ctctggaggg
     9481 gccattttgc gcggcctgag acgttgcagc tacacgacga tctgggcagg attaacttct
     9541 cgtgcatcct ggaaggcaca tcgcgctttg cgattcaggg actccggcgc cacaccgact
     9601 gggagcttgc gcgcaatcgc cattacatta cccatacgcc agactgtcgc ggcagcgcct
     9661 cctattgtgg ccgattcgag agcattaccc tctccttcag ccctgaaacc ctcgcgctgt
     9721 gggtgccgga tattagcgcg gtcattaaga ataagatcga ctcccactgc tgttgccagc
     9781 agcagcgttg taatgctgaa acccacttaa cggctcaggc cttgcgtcac gcgctgatgc
     9841 gcatgcacga cggcttcaat catgaacaaa agccgtcgac gctatggttg caggggcaaa
     9901 gtctggtcat gctcagtctg gtgctggatg aacatcgcga agacgccagt tgcctctcct
     9961 gccacttcaa cccgatggaa cgccagaaac tgctgcgggc gaaggatctg ctgctggctg
    10021 atctgacgca agcgccgggc gtcgcggagc tggccagaga gtccggtctg agcgttctca
    10081 aaattaagcg aggcttccgc gttctgttca acaacagcgt ctatggcctg tttcaggctg
    10141 aacgaatgca ggaagccaga agacgcctgg ccaacggcaa tacctccgtc atgacggtgg
    10201 cggccgatct gggctatgcc aacgccagcc atttctctgc cgccttccag aaacagtttg
    10261 gcgtaactcc ttcgaccttt aaacgcggga tgtaaccggg gtcgcgcccc cctaaactcc
    10321 tccctgacag aggcccggat accccatccg ggcctctgtt ctttccgcct gttaccaacc
    10381 cgaaacgggc ataaataaac ccgtttcggg tagcatccaa ttgttaataa ttattattct
    10441 catatgagta atgcttttcg gtaagacgtg ccatcaggag gaagaatgat ttctggcgca
    10501 ccatctcagg attcgctgtt accggacaac cgccacgcgg ctgattacca acaattacgc
    10561 gagcggctca tacaggaact gaatttaacg ccgcagcagt tacatgaaga gagcaacctg
    10621 atccaggccg gtctggattc cataagattg atgagatggt tacactggtt tcgtaaaaat
    10681 ggctaccgcc ttacccttcg cgagctgtat gccgccccca cgctggcggc atggaaccag
    10741 ttaatgctca gccggtcgcc ggagaacgcg gaagaagaaa cgccgcccga cgaatcatcc
    10801 tggccgaaca tgaccgaaag tacccccttc ccattgacgc cagtacagca cgcctacctg
    10861 acgggccgca tgccggggca gacgcttggc ggcgtgggtt gccacctgta tcaggagttt
    10921 gaaggccatt gtctgacggc gtcgcagctg gagcaggcca tcacgacctt gctgcaacgc
    10981 cacccaatgc tgcatatcgc ctttcgcccc gacgggcagc aggtctggct accgcaacct
    11041 tactggaacg gcgtcaccgt tcatgattta cgccataacg acgctgaaag ccgccaggcc
    11101 tatctggacg cactgcgcca gcgcctgagc caccgtcttt tacgcgtgga aatcggcgaa
    11161 acgtttgatt ttcagctgac gctcttgccg gacaatcgcc accgcctcca tgtcaatatt
    11221 gacctgctga ttatggatgc ctccagcttt acgcttttct tcgatgagct taacgccctg
    11281 ctggccggag aatcgctgcc ggctatcgac acccgctatg atttccgctc gtatttgctg
    11341 caccagcaga agatcaatca accactgaga gacgtcgcgc gcgcttactg gctggcgaaa
    11401 gcatcgacgc ttccccccgc gcccgtcttg ccgctggcct gcgaacccgc cacgctacgt
    11461 gaagtccgta atacccgacg ccgcatgatt gtcccggcaa cacgctggca cgcctttagc
    11521 aaccgggccg gcgagtatgg cgtgacgccg acaatggcac tggcgacctg tttttctgcc
    11581 gtgctggctc gctggggcgg cctgacgcgt ctgctgctta acatcacctt attcgaccgc
    11641 cagccgctgc aaccggcggt tggcgcgatg cttgccgact tcaccaatat tcttctgctg
    11701 gataccgcct gcgatggcga taccgtcagc aacctggcgc gtaaaaacca gctcacgttt
    11761 acggaggact gggagcatcg ccactggtcc ggcgtcgaat tactccgtga actcaaacgc
    11821 cagcagcgct acccccacgg cgccccggtg gtatttacca gcaatctggg gcgttccctc
    11881 tacagcagcc gcgcagaatc gccgttgggc gagccggaat ggggcatctc gcaaacgccg
    11941 caggtctgga tagatcatct ggcgttcgag catcacggcg aggtctggct acaatgggac
    12001 agcaacgacg cgctgttccc tccggcgtta gtcgaaacat tgttcgacgc ctactgccag
    12061 ttgattaacc aactctgcga tgacgaaagc gcctggcaaa agccgttcgc agatatgatg
    12121 cccgccagcc agcgcgcgat acgcgaacgg gtcaacgcca ccggcgcccc cattcccgaa
    12181 ggcttgctgc atgaaggcat tttccgtatc gctctgcaac agccgcaggc gctggcggta
    12241 acggacatgc gttatcagtg gaattatcat gagctgacag actatgcccg ccgttgcgcg
    12301 ggcaggttaa tcgagtgcgg ggttcagccc ggcgataatg tggctatcac gatgtcgaaa
    12361 ggcgcaggac aacttgttgc ggttctggcc gtcctgctgg ccggggcggt ttacgttccg
    12421 gtttcgctgg atcagcctgc cgcacggcgc gagaaaatct acgctgacgc cagcgtccgg
    12481 ctggtgctca tttgtcagca cgacgccagc gccgggtcag acgatattcc cgcccttgcc
    12541 tggcagcagg ccattgaggc ggagccgatc gccaacccgg tagtacgcgc ccccacgcaa
    12601 ccggcctaca ttatctacac ctccggctct accggtacgc cgaaaggggt agtcatttct
    12661 caccggggag cgcttaacac ctgttgcgat atcaataccc gctatcaggt tggcccgcat
    12721 gacagggtgc tggccctctc cgccctacat tttgatttat cggtttacga catttttggc
    12781 gtactgcgcg cgggcggcgc gctggtgatg gtgatggaaa atcaacggcg cgatcctcac
    12841 gcatggtgtg agctgatcca gcgccatcag gtcacgctct ggaacagcgt cccggcgctg
    12901 ttcgatatgc tgctgacctg gtgtgaaggt ttcgccgacg ccacgccgga aaacctgcgc
    12961 gcagtgatgc tttccggcga ctggatcggg cttgacctcc ccgcccgtta tcgggccttc
    13021 cggccacaag gacaatttat cgcgatgggc ggcgccaccg aggcgtctat ctggtctaac
    13081 gcctgcgaaa ttcacgacgt ccccgcctac tggcgctcca tcccttacgg ttttccgcta
    13141 accaaccaac gctaccgggt ggtggatgaa cagggccggg actgccctga ctgggtgccg
    13201 ggtgaattat ggattggcgg cattggggtc gcggaaggct atttcaacga tcccctgcgt
    13261 agcgagcagc aatttttgac gctcccggac gagcgctggt atcgcaccgg cgatctcggc
    13321 tgctactggc cagatggcac aatcgagttc ctcggtcgtc gcgacaagca ggtcaaagtc
    13381 ggaggatatc gcatcgagct gggcgaaatc gaaagcgcgc tcagccagct ggtgggggtg
    13441 aaacaagcaa ccgttctggc gatcggcgaa aaagaaaaaa cgctggcggc atacgttgtt
    13501 cctcagggcg aggctttttg cgttaccgat catcggaacc cggcactgcc gcaggcgtgg
    13561 cacacgcttg cgggaacgtt gccctgttgc gccatctcgc cagagatctc cgcagaacag
    13621 gtagccgatt tccttcagca tcgcctgcta aaactgaagc cgggtcacac cgctggcgcc
    13681 gatcctctcc ccctgatgaa ctcactcgct atccagccgc gctggcaggc cgtggtggaa
    13741 cgctggttag catttctggt gacacaacgg cgactgaagc ccgctgctga aggttatcag
    13801 gtctgcgctg gtgaagaacg cgaggatgag cacccgcact tcagcggaca tgatttaacg
    13861 ttatcgcaaa ttcttcgcgg tgcccgtaac gaactgtcgt tactgaacga cgcgcagtgg
    13921 tcgccggaaa gcctggcctt taaccatccg gccagcgccc cgtatattca ggaactggcg
    13981 acaatttgcc aacagcttgc acagcgctta cagcgcccgg tacgcctgct tgaggtggga
    14041 acccgcactg gccgcgccgc agaatcgctg ttagcacagc tcaacgccgg acagattgag
    14101 tatgtcgggc ttgagcagag ccaggagatg ctgctgagcg cccggcagag gctcgccccc
    14161 tggcctggcg cccgtctgtc cctctggaat gcagacacgc tggcggcgca cgctcactcg
    14221 gcggacatta tctggcttaa taacgccctg catcgtctgc tgccggaaga tcccgggctc
    14281 cttgcgacat tacaacagct tgccgttccc ggcgcgctgc tctacgtgat ggagtttcgc
    14341 cagttaacgc cgtccgccct actcagcacg ctcctgttaa ccaatgggca gccggaggcc
    14401 ttgctgcata acagcgccga ctgggcggca ttatttagcg cggccggctt caactgtcag
    14461 catggcgatg aggtcgcggg gttacaacgc ttcctcgtac aatgtcctga caggcaggtg
    14521 cgccgcgatc cccgtcaact tcaggccgcc ctcgccgggc gtctgccggg gtggatggtg
    14581 ccgcaacgga tcgtcttcct cgacgcctta ccgctgacag ctaacgggaa aattgactac
    14641 caggcgctga agcgtcgtca tacccctgaa gcggaaaacc cggccgaagc ggatttaccc
    14701 cagggcgaca ttgaaaaaca ggttgccgcc ctctggcagc aactcttatc aactggcaat
    14761 gtcaccagag aaaccgactt cttccagcaa ggcggcgata gcctgctggc gacccgtctg
    14821 accgggcaac ttcatcaggc aggttatgaa gcgcaattaa gcgacctgtt taatcatccc
    14881 cggctggcgg attttgccgc cacgctgcgg aaaaccgacg tcccggtcga acaaccattc
    14941 gtccactccc ctgaagatcg ctaccagccc tttgcgctta ccgacgtgca gcaggcttac
    15001 ctggtggggc gtcagccggg ctttgccctg ggcggcgtcg gctcacattt ctttgttgaa
    15061 tttgaaattg ccgatctgga cctcacccgg ctggagacgg tctggaaccg attaatcgcc
    15121 cgccacgata tgctgcgcgc catcgtgcgt gatggacagc aacaggtgct cgaacagacg
    15181 cccccctggg tgatacccgc acacaccctc catacgcctg aagaggcgtt gcgggtgcgc
    15241 gaaaaactgg cgcatcaggt actcaacccc gaagtatggc cggtattcga tctccaggtc
    15301 ggatacgtgg acgggatgcc tgcccgcctg tggctgtgtc tggataacct gttgcttgac
    15361 ggtctgagca tgcagatcct gctggcggag ctggagcacg gctaccgcta cccgcaacag
    15421 ctgcttccgc cgctgcccgt caccttcagg gattatctgc aacaaccctc gctacagtcg
    15481 cccaatccag attctctggc atggtggcag gcgcagcttg atgatattcc tccggcgcct
    15541 gcgttgccgc tgcgctgctt gcctcaggag gttgaaacac cgcgcttcgc ccgcctgaac
    15601 ggcgcactgg acagcacgcg ctggcatcgg ctgaaaaaac gggcggctga cgcccatctc
    15661 accccgtcgg ccgtactgtt gtcggtgtgg tcaacggttc tctctgcatg gagtgcacag
    15721 cctgacttca cgcttaacct tacgcttttc gacaggcgac cgctgcaccc gcaaatcaac
    15781 cagattctgg gcgatttcac ctcgctgatg ctgctgagct ggcatcccgg cgaaagctgg
    15841 ctgcacagcg cgcagtcact acagcagcgg ctgagccaga acctcaacca ccgcgatgtg
    15901 tcagccatcc gcgtgatgcg tcaactggcg caacggcaaa acgtgccagc cgttccgatg
    15961 cccgtcgtct ttaccagcgc gctgggcttt gagcaggata acttcctcgc ccggcgtaat
    16021 ctgctcaaac cggtctgggg catctcccag acgccgcagg tctggctcga tcaccagatt
    16081 tatgaatccg aaggcgaact gcgctttaac tgggattttg tcgccgcgct gtttcctgcc
    16141 gggcaggtgg agcgccagtt tgaacagtat tgcgcattgc taaaccgaat ggccgaggat
    16201 gaaagcggct ggcaactgcc gctcgccgcg ctggtgcctc ccgttaaaca cgcagggcaa
    16261 tgcgcagagc gctcaccgcg cgtatgccct gagcactctc agccacacat tgcggcggac
    16321 gagagcaccg tcagcctgat ttgcgacgcc ttccgcgagg tggttggcga gtctgtcacg
    16381 cccgcagaaa acttctttga ggcgggcgca acgtcgctga atctggtgca actgcacgtt
    16441 ttgttacaac gtcacgaatt ttccaccctg acgttgcttg acctcttcac ccacccttct
    16501 cctgctgccc tggccgatta tctggccggc gtcgccacgg tggagaaaac acaacgacct
    16561 cgccctgttc gccgtcgtca gcggcggata tagcgcgaag caaactgatt ttccccggaa
    16621 cgccatcgcg aacgcatggc gttccattga ctttgtgaat cttaggaaac gggaccgatt
    16681 atggataact tgcgcttctc ttctgcgccg acagcagatt ccattgatgc atcgatcgct
    16741 caacactacc cggactgcga acctgtcgcg gttatcggct acgcctgcca ttttcctgaa
    16801 tcgccggatg gcgaaacgtt ctggcaaaat ctgctggaag gtcgtgaatg cagccgacgc
    16861 tttacgcgcg aagagcttct ggccgtcggt ctggatgccg ccatcattga cgatcctcat
    16921 tatgtcaata tcggttcggt gttagacaac gccgactgct tcgacgccac cctgtttggc
    16981 tattcgcgac aggaagcgga gtcgatggac ccgcagcagc gcctgttttt gcaggcggtc
    17041 tggcatgcgc tggaacatgc cggttatgcc cccggcgccg tcccccataa gaccggcgtt
    17101 ttcgcctctt cccggatgag tacctacccc ggtcgcgaag cattgaacgt gacagaagtc
    17161 gcgcaggtaa aaggtctgca atctctgatg ggcaatgata aagactatat tgccacccgc
    17221 gccgcgtaca aactcaacct gcacggcccg gcgttatcgg tacagaccgc ctgctccagc
    17281 tcgctggttg ccgtgcatct ggcctgtgaa agcctgcgcg ctggcgaatc cgatatggcg
    17341 gttgccggcg gcgtggcgct ctctttcccc cagcaggcag gctaccgcta ccagcccgga
    17401 atgattttct ctcctgatgg tcactgtcgt ccctttgacg cctcggctga gggcacctgg
    17461 gccggtaacg gtctcggctg cgtggtgctg cgtcgcctga gagacgcgct gctgtcaggc
    17521 gatccgatta tctcggtgat cctctccagc gcggtcaaca acgacggcaa cagaaaggtc
    17581 ggctataccg ccccttccgt cgcagggcaa caggcagtca tcgaagaggc gttaatgctg
    17641 gcggccatcg acgacaggca ggtaggttac attgaaaccc acggcaccgg cacaccgctg
    17701 ggcgacgcga ttgaaattga agcgttacgc aacgtctatg cgcctcgccc gcaggatcag
    17761 cgctgtgcgc tcggttccgt gaaaagtaac atgggccatc tggataccgc ggcgggcatt
    17821 gccggactgc tgaaaaccgt tctggcagtc agtcgcgggc aaattcctcc cttactgaat
    17881 tttcacaccc ccaacccggc gctgaaactt gaagagagcc cctttaccat accggtgtcg
    17941 gcacaggcat ggcaggacga aatgcgctat gcgggcgtct cctcctttgg tattggcggc
    18001 accaactgcc atatgatcgt cgcctcgctg cccgacgcgc tcaacgcgcg cctccccaat
    18061 acggatagcg gcagaaaaag taccgcgctg ctgctcagcg ccgccagcga cagcgcgttg
    18121 cggcggctgg cgacggatta tgccggggcg ctgagagaga atgcggatgc cagctctctg
    18181 gccttcacag ccctgcacgc gcgccgtctc gatctcccct tccgcctggc ggcgccatta
    18241 aaccgtgaaa ccgccgaggc gctcagcgcc tgggccggtg agaaatcggg ggcgctggtt
    18301 tacagcggcc acggcgccag cggcaagcag gtgtggctgt ttaccggcca gggctcgcac
    18361 tggcacacta tgggccaaac gatgtaccag cactcaacgg cgtttgccga cacgctggat
    18421 cgctgttttt ccgcctgtag cgaaatgctc acgccatcac tgcgcgaagc gatgtttaac
    18481 cccgattcgg cgcagctgga caatatggcc tgggcgcagc cggcgattgt cgcgtttgaa
    18541 atcgcgatgg cggcgcactg gcgtgctgaa ggactgaagc cagacttcgc cattgggcat
    18601 tccgtcggtg aatttgccgc tgccgttgtc tgcggacact atacgattga acaggtcatg
    18661 ccactggttt gtcggcgcgg cgcgctaatg cagcagtgcg caagcggcgc gatggtggcg
    18721 gtatttgcag acgaagacac gctgatgccg ctggctcgcc agtttgagct ggatctcgcc
    18781 gccaacaacg gtacgcaaca tacggtattt tccgggccgg aagcccgtct cgcggtattt
    18841 tgcgccacgc tctcgcagca tgacattaac tatcgtcgcc tgagcgtaac cggtgcggcg
    18901 cactccgctt tactggagcc gatactcgat cggttccagg acgcctgcgc gggactgcac
    18961 gcggagccgg ggcaaatacc gattatttcc acgctcaccg ccgacgtcat tgatgagtca
    19021 acgctcaacc aggcggatta ctggcgccga cacatgcgcc agccggtgcg ttttatccag
    19081 agtattcagg tggcgcatca gctcggcgcc cgcgtttttc tggagatggg gcccgatgcc
    19141 cagttggttg cttgcgggca gcgcgaatac cgcgataacg catactggat agccagcgcc
    19201 cggcgtaaca aagaggcgag cgatgtcctc aatcaggccc tgctccagct ttacgctgcc
    19261 ggcgtcgccc taccgtgggc cgacctgctg gcgggcgatg gacaacgtat cgctgcgcca
    19321 tgttatccgt ttgatactga acgttactgg aaagagcgcg tctccccggc ctgcgagcct
    19381 gccgacgcag cgctgtctgc cgggctggag gtggcgagtc gcgccgcgac agcgctcgat
    19441 ctccctcgcc tggaagcgct taaacagtgc gccacgcgac tgcacgccat ctacgtcgat
    19501 caactggtac aacgctgtac cggcgatgcc attgagaacg gcgtggacgc catgaccatc
    19561 atgcgccgtg gacgtctgct gccccgctac cagcagctac tccagcgcct gctgaataac
    19621 tgcgtggtcg acggcgatta ccgctgcacc gacgggcgat acgtccgcgc ccgccccatt
    19681 gaacatcaac agcgggaatc actgctgacg gaacttgcca gttattgtga aggttttcag
    19741 gctattcccg acaccatcgc ccgtgccggc gatcggttat atgaaatgat gagcggcgcg
    19801 gaagaaccgg tggcgattat cttcccgcaa agcgcctccg acggcgtgga agtgctgtat
    19861 caggaattca gctttggccg ctatttcaac caaatcgccg ccggggtatt acgcggcatt
    19921 gtccagacgc gtcagccccg ccagtcgttg cgtattcttg aagttggcgg cggaaccggc
    19981 ggcaccaccg cgtggctgct gccggaactc aacggcgttc cggcactgga gtaccatttc
    20041 accgatatct cggcgctgtt cacccgtcgc gcccagcaga aattcgccga ctatgatttt
    20101 gtgaagtata gcgagctgga tctcgaaaaa gaggcgcagt ctcagggttt ccaggcacag
    20161 tcttacgatc ttatcgtggc agcgaacgtg attcacgcca cccgccatat tggccgcacg
    20221 ctcgataatc tgcgccccct gctcaagccg ggcgggcgcc tgctgatgcg cgaaatcacc
    20281 cagccaatgc gtctgtttga cttcgttttc ggcccgctgg ttcttccgct acaggatctc
    20341 gacgcccgcg aaggtgagtt attcctcacc accgctcagt ggcaacaaca gtgccgccac
    20401 gccggattca gcaaagtggc gtggctaccg caggatggca gcccgaccgc cgggatgagc
    20461 gaacatatca ttctcgccac gctgcccggt caggcggtta gcgccgtaac attcaccgcg
    20521 ccatcagaac ccgtgttggg gcaggcgctg acggataacg gtgattatct cgccgactgg
    20581 tctgattgcg caggtcagcc cgaacagttt aacgcccgct ggcaggaggc ctggcgtctg
    20641 ctttcacagc gtcatggcga cgctctgcct gtggaaccgc cccccgtcgc cgccccggag
    20701 tggctgggga aggttcgctt aagctggcaa aacgaagcct tttcccgcgg tcagatgcgc
    20761 gttgaagccc gtcatcctgc tggcgagtgg ctgccgctat cgcccgccgc gcctcttcct
    20821 gcgccgcaaa cgcattatca atggcgctgg acgcccctca acgtcgccag cattgaccat
    20881 ccgcttacct ttagcttcag cgccggtacg cttgcgcgca gcgacgagct ggcgcaatac
    20941 ggcatcattc acgatccgca cgcctcttca cgactgatga ttgttgagga gagcgaggat
    21001 acgctggcct tagcggagaa agtgatagca gcgctcaccg ccagcgcagc cggattgatt
    21061 gtggttactc gccgcgcgtg gcgagtcgag gaaaatgaag cactctctgc atcccatcac
    21121 gcgctatggg ccttgcttcg cgtcgcggcc aacgaacagc cggaacggtt gcttgccgcc
    21181 atcgatctcg ccgaaaacac cccgtgggaa acgctgcatc aagggttgag cgcagtctca
    21241 ctatcacagc gctggctcgc cgcacggggt gacacccttt ggctcccttc actggcgccc
    21301 aatacgggat gcgcagctga attaccggca aacgtgttta ccggcgatag ccgctggcat
    21361 ctggtgaccg gagcgtttgg cggattaggc cgccttgccg tgaactggct cagagaaaaa
    21421 ggggcgcgac gcatcgccct gctggcatcg cacgtggatg agtcatggct acgcgacgtg
    21481 gagggcgggc agacgcgcgt ctgccgttgt gatgtgggcg atgccgggca actggccacg
    21541 gttcttgacg atctggcggc caacggcggc attgccggag cgattcatgc cgctggcgta
    21601 ttggctgacg cgcccttgca ggagcttgat gaccaccagc tggctgccgt tttcgcggta
    21661 aaagcgcagg cggcaagcca gctgttgcaa accctgcgca accacgacgg acgctatctt
    21721 attctctact cttccgctgc cgccaccctc ggcgcgccgg gtcagagcgc ccatgcgctg
    21781 tcctgcggct acctggacgg gctggcccag cagttttcca cccttgatgc gccgaaaacg
    21841 ctctctgtcg cctggggcgc atggggagaa agcggtcggg cggccacgcc ggaaatgctg
    21901 gcgacgctcg ccagccgagg tatgggcgcg ttaagcgatg ccgaaggctg ctggcacctg
    21961 gaacaggcgg tgatgcgcgg cgccccgtgg cgactggcga tgcgcgtttt taccgacaaa
    22021 atgcccccgt tacaacaggc tctgtttaac atcagcgcca cagaaaaagc cgcaacgccg
    22081 gtcattcctc ctgctgatga caacgccttt aacggcagcc tgagcgatga aacagcggtg
    22141 atggcatggc tgaaaaagcg gattgcggtt cagctaaggc tgagcgatcc ggcgtcactg
    22201 catccaaacc aggatctgtt gcaactcggc atggactcgc tgctcttcct tgaactcagt
    22261 agcgatattc agcactacct gggcgtacgc atcaatgcgg aacgggcgtg gcaggatctg
    22321 tctcctcatg gactcacgca gcttatctgt tctaagccag aggcgacgcc tgccgtttcg
    22381 cagccggaag tgttgcggca cgacgccgac gagcgttatg cgcccttccc tttgacgccc
    22441 attcagcacg cctactggct ggggcgaacc cacctcattg gctatggcgg cgtcgcctgt
    22501 cacgtcctgt ttgagtggga taaacgccac gatgagttcg atctagccat actggagaaa
    22561 gcatggaacc agctcatcgc acgccacgat atgttgcgta tggtggttga tgccgacggg
    22621 cagcagcgaa tcctggcgac aacgccggag tatcacatcc cgcgtgacga tctgcgcgcg
    22681 ctttccccgg aagaacagcg catcgcgctg gaaaaacggc ggcatgaact gagctatcgc
    22741 gttttgcctg ccgaccagtg gcctcttttt gagctggtgg tcagcgaaat cgacgattgc
    22801 cattaccgtc tgcatatgaa cctcgacctt ttgcagtttg atgtgcagag ttttaaagtc
    22861 atgatggacg acctggcgca ggtctggcgc ggtgaaacgc tggcgccgct cgctattacc
    22921 ttccgtgatt atgtgatggc tgaacaggcg cgccgaaaga catcggcatg gcacgatgcc
    22981 tgggattact ggcaggaaaa actgccgcaa ctgcccttag cgccagagct gccgatggtt
    23041 gagacgcccc cggaaacgcc acacttcacc accttcaaat cgacgatcgg caagacagaa
    23101 tggcaggccg tgaaacagcg ctggcagcag caaggcgtca caccgtctgc cgcgctgctc
    23161 acgctgtttg ccgccaccct tgagcgctgg agccgtacca caacatttac gctgaacctg
    23221 acgttcttca atcgccagcc gatccatccg caaatcaacc agttgattgg tgattttacc
    23281 tccgtcacgc tggttgattt taacttctca gcgccagtga cgttgcaaga gcagatgcaa
    23341 cagacccaac agcgcctctg gcaaaatatg gcgcacagtg aaatgaacgg tgttgaggtg
    23401 atccgtgagc tgggccgcct gcgcggatca caacgtcaac cgctgatgcc ggtagtgttt
    23461 accagtatgc tggggatgac gctggaaggc atgactatcg atcaggcgat gagccatctg
    23521 ttcggcgaac cctgctatgt attcacgcaa acgccgcagg tctggctgga tcatcaggtc
    23581 atggagagcg acggcgagtt gatgtttagc tggtactgca tggacaacgt gctggaaccc
    23641 ggcgctgccg aggcgatgtt taatgactat tgcgccatcc tgcaagccgt catcgccgcc
    23701 cctgaaagcc tgaagactct cgccagcggc atcgccgggc acattccccg ccgacgctgg
    23761 ccgctgaacg cgcaggcgga ctacgacctg cgggatattg agcaggcgac gctcgaatac
    23821 cccggcatcc ggcaggccag agcggaaata accgaacagg gcgcgttgac gctggatatc
    23881 gtgatggccg acgatccttc gccatcagcg gcgatgcctg atgagcacga acttacccaa
    23941 ctggcgctgc cgttgcctga gcaggcgcag cttgatgagc tggaggcgac ctggcgctgg
    24001 ctggaggcgc gtgcgctaca ggggatcgcg gctacgctaa atcgtcacgg cctgtttacc
    24061 acgccggaga tcgcccatcg ctttagcgca atagtacagg cgctgtccgc gcaagcgtct
    24121 caccagcgtc tgctgcgcca gtggctacag tgtctgacgg aaagagagtg gttaatccgc
    24181 gaaggtgaaa gctggcgctg ccgcattccg ctcagcgaga ttcctgagcc tcaggaagcg
    24241 tgcccgcaaa gccaatggag ccaggcgctg gcgcagtatc tggaaacctg catcgcccgg
    24301 cacgacgccc tcttctccgg gcagtgttct ccgctggaat tgctgttcaa cgagcagcat
    24361 cgcgttaccg acgcgctgta tcgcgacaac cccgccagcg cctgtctgaa tcgctatacc
    24421 gcgcagattg ccgccttgtg cagcgcagaa cggattctgg aggttggcgc cggaaccgca
    24481 gccactaccg cgccggtgct gaaggccacg cggcagtcgt accacttcac ggacgtctcc
    24541 gcgcagttcc tcaatgacgc cagagcccgt ttccatgatg aatcgcaggt gtcttatgcc
    24601 ttgttcgaca tcaaccagcc gctggatttc accgcccacc cggaggcggg ttacgacctg
    24661 atcgttgccg tcaatgtgct ccacgacgcc agccatgtcg tccagacgtt gcgcagatta
    24721 aaactgttgc tgaaagccgg cggacgtttg ctgatcgttg aagcgacgga gcgaaacagc
    24781 gtattccagc tggcgagcgt gggctttatt gagggattaa gcggataccg cgatttccgc
    24841 cgccgggatg agaaaccgat gctcacccgc tccgcatggc aggaggttct cgttcaggcc
    24901 gggtttgcaa acgagctggc gtggcccgcg caggaatcgt cgccgctgcg ccagcatctg
    24961 ctggtagcgc gttcgcctgg cgtaaatcgc ccggataaaa aagccgtgag ccgctattta
    25021 cagcagcgct ttggcaccgg tctgcccatt ttacagatcc ggcaaagaga agcgttattt
    25081 acgccgctgc atgccccgtc tgatgcgccg actgagccag ccaaacccac gccagttgcc
    25141 ggggggaatt cggcgctgga aaaacaggtg gctgaactct ggcaatcgct gctgtctcgc
    25201 cccgtggcaa ggcatcacga ctttttcgaa ctgggcggcg acagcctgat ggcgacaagg
    25261 atggtcgcgc agctaaaccg gagagggatt gccagggcta accttcagga tctgttcaac
    25321 cattcgacgc tgagcgactt ctgcgcccat ctacaggcgg ctacgtcagg agaggacaac
    25381 ccgatacccc tttgccaggg cgacggtgag gaaaccctgt ttgtcttcca cgcttcagac
    25441 ggcgatatca gcgcctggct gccgctcgct agcgcgttga acaggcgcgt tttcggcctg
    25501 caagcaaaat cgccgcagcg ctttgctacg ctcgaccaga tgatcgatga gtatgtcggg
    25561 tgcatccgtc gtcagcagcc tcacggccct tatgtgctgg cgggttggtc gtatggcgcg
    25621 tttcttgcgg cgggcgccgc acagcgcctg tacgccaaag gcaagcaggt tcggatggtg
    25681 ttaatcgatc ccgtgtgccg acaggatttc tgttgcgaaa accgggcggc cctgctgcgc
    25741 ctgttagccg aaggagaaac gcctctggca ctgcccgaac atttcgacca gcagacgccc
    25801 gacagccagc ttgccgactt tatcagcctc gctaaaacgg ccggtatggt gtcgcaaaac
    25861 ctgacgctgc aagcggcaga aacgtggctc gacaacatcg cgcatctgct gcgtttactg
    25921 actgagcata cgccgggcga aagcgttccg gtcccctgtc tcatggtgta tgccgccggg
    25981 agacccgcgc gctggacgcc agcagaaacc gagttgcagg gctggataaa caacgccgac
    26041 gacgctgtga ttgaagccag ccactggcaa atcatgatgg aagcccccca cgttcaggct
    26101 tgtgcgcaac acattacgcg ctggctttgc gcaacctcaa cgcaaccgga gaacacgtta
    26161 tgatgccgtc cgcctcccca aaacaacgcg tactgattgt gggcgccaaa tttggcgaaa
    26221 tgtacctgaa tgcctttatg cagcccccgg aggggctgga actggtcggc ctgctggcgc
    26281 agggaagcgc ccgttcaaga gagctggctc atgcgtttgg cattccgctg tatacctcgc
    26341 cggaacagat aaccaggatg ccggatatcg cctgtattgt cgtgcgttcg accgtcgccg
    26401 gaggaaccgg tacgcagctt gccagacact ttctgacgcg cggcgtacac gtcattcagg
    26461 agcatcccct ccacccggat gacatcagtt ccttacaaac gctggcgcag gaacagggct
    26521 gctgctattg ggtaaacacg ttttatccgc atacgcgcgc gggacgcaca tggcttcgcg
    26581 atgcgcagca gctgcgtcgc tgcctggcta agacgccgcc ggtagtccac gccaccacca
    26641 gccgacagtt gctctactcc actctggatt tgttgttgct ggctctgggc gttgatgccg
    26701 ccgccgtgga gtgcgacatg gttggcagct ttagcgattt tcactgtctg agactcttct
    26761 ggccggaggg agaagcctgc ctgctgcttc agcgctatct cgatcctgac gatccagata
    26821 tgcatagcct tatcatgcac cgcctgctgc tgggctggcc ggaaggtcat ctttcgctgg
    26881 aggcgagcta cggccccgtc atctggtcat ccagtctgta tgtcgcagac catcagcaga
    26941 acccccacag tctctggcgc agaccggaga tcctgcgcga tctgccgggt ctgacgcgca
    27001 gcgcggcgcc cctgagctgg cgcgactgct gcgaaaccgt cgggccggag ggcgtcagct
    27061 ggttgctaca ccagttgcgc agcaatctgg cgggcgaaca tccaccagag gcctgccaaa
    27121 gcgtacatca gatcgcgtta tcacgtttat ggcagcagat tttacgtaag accggcaatg
    27181 cggagatcag acgtctgacg ccgccgcacc acgaccggtt agccggtttc tacaacgatg
    27241 atgataagga ggcgctgtga cgcaatctgc aatgtgcatc ccgctgtggc ccgcccggaa
    27301 cggcaatact gcgcatctgg tcatgtgccc tttcgctggc ggcagcagta gcgcgtttcg
    27361 ccactggcaa gctgagcaac tggctgattg cgcgctttct ctggtgacct ggccggggcg
    27421 cgatcgcctt cgccatctgg aaccgctcag aagcattaca caactggcgg cactgctggc
    27481 gaacgagctg gaagcatccg tatcgcctga cacgccgctt ttactcgccg ggcacagcat
    27541 gggggcgcag gtggcgtttg aaacctgccg acttctggag caacgggggc ttgcgccaca
    27601 aggactgatt atttccgggt gccatgcccc gcatctgcat tctgaacgcc agctcagcca
    27661 tcgcgatgat gccgacttta tcgctgagct gatagacatt ggcggatgtt ctcctgaact
    27721 gcgggaaaac caggaattaa tgtcgctgtt tcttcctctt ctgcgcgctg atttttacgc
    27781 caccgagagc tatcactacg actcgcccga cgtctgtccg ccgctgcgca cgcctgcgct
    27841 gttattgtgc ggcagccacg atcgcgaagc ctcctggcag caggtcgatg cctggcgtca
    27901 gtggctgagc cacgttacag gcccggtggt gattgacggc gatcacttct atcccattca
    27961 acaagcccgg tcctttttta cgcagattgt ccgccatttt ccccacgcat tttctgcaat
    28021 gaccgcgtgg caaaaacagc ccagtacttc agaaaggtga cgcatgaatt cttcctttga
    28081 atctctgatt gaacagtatc ccttacccat tgccgaacag ttgcgccact gggcggcccg
    28141 ttatgcctcg cgaattgccg tcgttgatgc aaaggggtcg ttaacctaca gcgcgcttga
    28201 tgcacaagtt gacgaacttg ccgcaggtct gtcatcactg ggtttgcgtt cgggggagca
    28261 tgtaattgtg cagcttccca acgacaacgc gtttgttacc ctgctgttcg ccttgttaag
    28321 actgggcgtt atccccgtgc tggcgatgcc ctcgcaacgg gcgctggata tcgacgcgct
    28381 gattgagctg gcgcaacccg tcgcttacgt tattcacggg gaaaaccacg cagagctggc
    28441 ccgacagatg gcacacaaac acgcctgctt gcgtcatgtt ctggtcgctg gagagaccat
    28501 gagcgacgat tttacgccgc tcttctccct tcacggtgag cgacagcctg atgtttccac
    28561 caccgcgttg ttgttgctct caggcggcac aaccggcacg cccaaactca tcccgcgccg
    28621 acatgccgac tatagctata acttcagcgc ttctgctgaa ctgtgcggca tcagccaaca
    28681 gagcgtgtat ctcgccgtcc tcccggtggc gcataacttt ccgctggcct gccccggtat
    28741 tctgggaacg cttgcctgcg gcggaaaagt ggtgctgacc gacagcgcca gctgtgatga
    28801 ggtgatgcct ttaatcgcgc aggaaagagt gactcacgtc gccctggttc cggcgctggc
    28861 gcaattatgg gtgcaggcca gggagtggga agacagcgac ctttcgtcgc tgcgcgtcat
    28921 tcaggcaggc ggcgcccggc tcgacccgac gcttgctgag caggttatcg ccacctttga
    28981 ctgtaccctg caacaggttt tcggtatggc ggaaggcctg ctctgtttta cccggctgga
    29041 cgatccgcat gccaccattc tccacagcca ggggcgcccg ttgtcccctc tggatgaaat
    29101 ctgcatcgtt gatcaagacg agaacgacgt cgcgccgggc gaaaccgggc aattgttaac
    29161 gcgcggccct tataccattt cgggctatta ccgcgcccct gcccacaacg cgcaggcctt
    29221 taccgcgcaa gggttttacc gcacaggcga caatgtcagg ctggatgagg tggggaacct
    29281 gcacgttgag ggacgcataa aagagcagat caaccgcgcc ggagaaaaaa tagccgcggc
    29341 tgaagtggaa tcggcactgc tgcgtttagc ggaagtgcaa gattgcgcgg tggtcgccgc
    29401 gccggacacg ctgcttggcg agcggatttg cgcgtttatc atcgcgcagc aggtgccaac
    29461 tgactatcag cagttgcgtc aacaactgac ccgtatgggg ctcagcgcgt ggaaaattcc
    29521 tgaccaaatc gagtttctgg accactggcc gctcaccgcc gtcggcaaga tagacaaaaa
    29581 acgcctgacg gctctcgccg tcgaccgtta tcgccattct gcccaataag cgcaaaccga
    29641 cccgaaacag gttgaaataa acccgtttcg ggtagcacca ctattagaaa tagttatcat
    29701 tttcaattca ccattgtcgg tatttttggc gtttcgccgt cttacaggga ctcacaacaa
    29761 tgaaaatgac acggctttat cctctggcct tggggggatt attgctcccc gccattgcta
    29821 atgcccagac ttcacagcaa gacgaaagca cgctggtggt taccgccagt aaacaatctt
    29881 cccgctcggc atcagccaac aacgtctcgt ctactgttgt cagcgcgccg gaattaagcg
    29941 acgccggcgt caccgccagc gacaaactcc ccagagtctt gcccgggctc aatattgaaa
    30001 atagcggcaa catgcttttt tcgacgatct cgctacgcgg cgtctcttca gcgcaggact
    30061 tctataaccc cgccgtcacc ctgtatgtcg atggcgtccc tcagctttcc accaacacca
    30121 tccaggcgct taccgatgtg caaagcgtgg agttgctgcg aggcccacag ggaacgttat
    30181 atggcaaaag cgctcagggc gggatcatca acatcgtcac ccagcagccg gacagcacgc
    30241 cgcgcggcta tattgaaggc ggcgtcagta gccgcgacag ttatcgaagt aagttcaacc
    30301 tgagcggccc cattgaggat ggcctgctgt acggcagcgt caccctgtta cgccaggttg
    30361 atgacggcga catgattaac cccgcgacgg gaagcgatga cttaggcggc acccgcgcca
    30421 gcatagggaa tgtgaaactg cgtctggcgc cggacgatca gccctgggaa atgggctttg
    30481 ccgcctcacg cgaatgtact cgcgccaccc aggatgccta tgtgggatgg aatgatatta
    30541 agggccgtaa gctgtcgatc agcgatggtt caccagaccc gtacatgcgg cgctgcactg
    30601 acagccaaac cctgagtggg aaatacacca ccgatgactg ggttttcaac ctgatcagcg
    30661 cctggcagca gcagcattat tcgcgcacct tcccttccgg ttcgttaatc gtcaatatgc
    30721 ctcagcgctg gaatcaggat gtgcaggagc tgcgcgccgc aaccctgggc gatgcgcgta
    30781 ccgttgatat ggtgtttggg ctgtaccggc agaacacccg cgagaagtta aattcagcct
    30841 acgacatgcc gacaatgcct tatttaagca gtaccggcta taccaccgct gaaacgctgg
    30901 ccgcatacag tgacctgacc tggcatttaa ccgatcgttt tgatatcggc ggcggcgtgc
    30961 gcttctcgca tgataaatcc agtacacaat atcacggcag catgctcggc aacccgtttg
    31021 gcgaccaggg taagagcaat gacgatcagg tgctcgggca gctatccgca ggctatatgc
    31081 tgaccgatga ctggagagtg tatacccgtg tagcccaggg atataaacct tccgggtaca
    31141 acatcgtgcc tactgcgggt cttgatgcca aaccgttcgt cgccgagaaa tccatcaact
    31201 atgaacttgg cacccgctac gaaaccgctg acgtcacgct gcaagccgcg acgttttata
    31261 cccacaccaa agacatgcag ctttactctg gcccggacgg gatgcagaca ttaagcaatg
    31321 cgggtaaagc cgacgccacc ggcgttgagc ttgaagcgaa gtggcggttt gcgccaggct
    31381 ggtcatggga tatcaatggc aacgtgatcc gttccgaatt caccaatgac agtgagttgt
    31441 atcacggtaa ccgggtgccg ttcgtaccac gttatggcgc gggaagcagc gtgaacggcg
    31501 tgattgatac gcgctatggc gcactgatgc cccgactggc ggttaatctg gtcgggccgc
    31561 attatttcga tggcgacaac cagttgcggc aaggcaccta tgccaccctg gacagcagcc
    31621 tgggctggca ggcgactgaa cggatgaaca tttccgtcta tgtcgataac ctgttcgacc
    31681 gtcgttaccg tacctatggc tacatgaacg gcagcagcgc cgtcgcgcag gtcaatatgg
    31741 gtcgcaccgt cggtatcaat acgcgaattg atttcttctg attattgtaa aagggatacc
    31801 gaaaaggtat cccttttaca ccactagtta aaaccagtaa ctcagcagag tcgcaaaaaa
    31861 tattaatcca tagtgattat ttaaaaaatg aaattgcgat taggataaat agatttaact
    31921 ttctcgttcc tttctctcct tatactaaag aaataatcat atcaaaataa aaattcacaa
    31981 cagtgcaaca ttaaaaaata caaccaacaa acaatcctat atacaaggca catctccaga
    32041 atataaaagc acagacaaac aacctaaaaa acaaccccat acatataaat tattttatag
    32101 gtaaaataga ttatatattc tcatagctac cataaactat actagcctga actatattta
    32161 ttctgctgca atcaatgcat acataacaca aatatcactc aggtacacta ctcaaaccac
    32221 gctgggattt ttcctcaagt tataatcatc accccgaaaa tcattcggca tttactcatt
    32281 aaatagtcac cccataggcc tgtacatgtt tactcagaaa tata
//
LOCUS       DAZKGS010000039        32243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKGS010000039 DAZKGS010000000
VERSION     DAZKGS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            334..633
                     /locus_tag="ACHOQY_001776"
     CDS             334..633
                     /locus_tag="ACHOQY_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677894.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(680..1567)
                     /gene="rarD"
                     /locus_tag="ACHOQY_001777"
     CDS             complement(680..1567)
                     /gene="rarD"
                     /locus_tag="ACHOQY_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9677895.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1619..2086)
                     /gene="yigI"
                     /locus_tag="ACHOQY_001778"
     CDS             complement(1619..2086)
                     /gene="yigI"
                     /locus_tag="ACHOQY_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9677896.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2251..3120
                     /gene="pldA"
                     /locus_tag="ACHOQY_001779"
     CDS             2251..3120
                     /gene="pldA"
                     /locus_tag="ACHOQY_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9677897.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3253..5082
                     /gene="recQ"
                     /locus_tag="ACHOQY_001780"
     CDS             3253..5082
                     /gene="recQ"
                     /locus_tag="ACHOQY_001780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9677898.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5146..5766
                     /gene="rhtC"
                     /locus_tag="ACHOQY_001781"
     CDS             5146..5766
                     /gene="rhtC"
                     /locus_tag="ACHOQY_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9677899.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5828..6448)
                     /gene="rhtB"
                     /locus_tag="ACHOQY_001782"
     CDS             complement(5828..6448)
                     /gene="rhtB"
                     /locus_tag="ACHOQY_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9677900.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6559..7581
                     /gene="pldB"
                     /locus_tag="ACHOQY_001783"
     CDS             6559..7581
                     /gene="pldB"
                     /locus_tag="ACHOQY_001783"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9677901.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7589..8389
                     /gene="yigL"
                     /locus_tag="ACHOQY_001784"
     CDS             7589..8389
                     /gene="yigL"
                     /locus_tag="ACHOQY_001784"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9677902.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8465..9364
                     /gene="bioP"
                     /locus_tag="ACHOQY_001785"
     CDS             8465..9364
                     /gene="bioP"
                     /locus_tag="ACHOQY_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9677903.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9252..10205)
                     /gene="metR"
                     /locus_tag="ACHOQY_001786"
     CDS             complement(9252..10205)
                     /gene="metR"
                     /locus_tag="ACHOQY_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9677904.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10323..12584
                     /gene="metE"
                     /locus_tag="ACHOQY_001787"
     CDS             10323..12584
                     /gene="metE"
                     /locus_tag="ACHOQY_001787"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9677905.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12623..13438)
                     /gene="ysgA"
                     /locus_tag="ACHOQY_001788"
     CDS             complement(12623..13438)
                     /gene="ysgA"
                     /locus_tag="ACHOQY_001788"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9677906.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13700..14461
                     /gene="udp"
                     /locus_tag="ACHOQY_001789"
     CDS             13700..14461
                     /gene="udp"
                     /locus_tag="ACHOQY_001789"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9677907.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14602..16029
                     /gene="rmuC"
                     /locus_tag="ACHOQY_001790"
     CDS             14602..16029
                     /gene="rmuC"
                     /locus_tag="ACHOQY_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9677908.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            16124..16879
                     /gene="ubiE"
                     /locus_tag="ACHOQY_001791"
     CDS             16124..16879
                     /gene="ubiE"
                     /locus_tag="ACHOQY_001791"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9677909.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16893..17498
                     /gene="ubiJ"
                     /locus_tag="ACHOQY_001792"
     CDS             16893..17498
                     /gene="ubiJ"
                     /locus_tag="ACHOQY_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9677910.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17495..19135
                     /gene="ubiB"
                     /locus_tag="ACHOQY_001793"
     CDS             17495..19135
                     /gene="ubiB"
                     /locus_tag="ACHOQY_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9677911.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19214..19483
                     /gene="tatA"
                     /locus_tag="ACHOQY_001794"
     CDS             19214..19483
                     /gene="tatA"
                     /locus_tag="ACHOQY_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9677912.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19487..20002
                     /gene="tatB"
                     /locus_tag="ACHOQY_001795"
     CDS             19487..20002
                     /gene="tatB"
                     /locus_tag="ACHOQY_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9677913.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            20005..20781
                     /gene="tatC"
                     /locus_tag="ACHOQY_001796"
     CDS             20005..20781
                     /gene="tatC"
                     /locus_tag="ACHOQY_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9677914.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20823..21605
                     /gene="tatD"
                     /locus_tag="ACHOQY_001797"
     CDS             20823..21605
                     /gene="tatD"
                     /locus_tag="ACHOQY_001797"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9677915.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21602..22090)
                     /gene="rfaH"
                     /locus_tag="ACHOQY_001798"
     CDS             complement(21602..22090)
                     /gene="rfaH"
                     /locus_tag="ACHOQY_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9677916.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22257..23750
                     /gene="ubiD"
                     /locus_tag="ACHOQY_001799"
     CDS             22257..23750
                     /gene="ubiD"
                     /locus_tag="ACHOQY_001799"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9677917.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23796..24497
                     /gene="fre"
                     /locus_tag="ACHOQY_001800"
     CDS             23796..24497
                     /gene="fre"
                     /locus_tag="ACHOQY_001800"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9677918.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24689..25852)
                     /gene="fadA"
                     /locus_tag="ACHOQY_001801"
     CDS             complement(24689..25852)
                     /gene="fadA"
                     /locus_tag="ACHOQY_001801"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9677919.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25862..28051)
                     /gene="fadB"
                     /locus_tag="ACHOQY_001802"
     CDS             complement(25862..28051)
                     /gene="fadB"
                     /locus_tag="ACHOQY_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9677920.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28241..29572
                     /gene="pepQ"
                     /locus_tag="ACHOQY_001803"
     CDS             28241..29572
                     /gene="pepQ"
                     /locus_tag="ACHOQY_001803"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9677921.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29572..30186
                     /gene="yigZ"
                     /locus_tag="ACHOQY_001804"
     CDS             29572..30186
                     /gene="yigZ"
                     /locus_tag="ACHOQY_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9677922.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30225..31676
                     /gene="trkH"
                     /locus_tag="ACHOQY_001805"
     CDS             30225..31676
                     /gene="trkH"
                     /locus_tag="ACHOQY_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9677923.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31688..32233
                     /gene="hemG"
                     /locus_tag="ACHOQY_001806"
     CDS             31688..32233
                     /gene="hemG"
                     /locus_tag="ACHOQY_001806"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9677924.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7494 a         8142 c         8803 g         7804 t
ORIGIN      
        1 tgatatcatt caaagttatt aatattgacc atgatgttaa tgtatagatt atgaactgaa
       61 agcgtgataa acgctcaagc ctggatgggt agaatatatt tctgatggat gatttgaaca
      121 tttttacatt gttttatata taattgtttt gaatatatat tacctgaatt atgtggtttg
      181 catatatata tgcaatatag cagtctttgc attgataact atacagtagt aatttagagt
      241 gtgtaagttt ttattgatgg ggtatatttt aattaaatat aattgaaatg tatttttatt
      301 ctataaacaa tatcttgggt gaatggattc tatatgggaa atggtaactt ttggcagttg
      361 ttcttgggta aagaacctta cagcaaaaaa tctgattttg ttaaggcatg gttattgtgg
      421 tgggctatta ccatccttgt tctatttatt ttaagcctga ctacgacatt aaatttaaga
      481 agtataggta tagggttaag ttttgggttt tgtcaaatcc ttggtagata tacgatgtct
      541 ctaggatgga gtaagtactg gggagttttg ggggtatttc ctgtaacaaa tattgttctt
      601 tttcttatct taattttttt caaaaaaaga taaataagtc agtgggtagg ctctttaaac
      661 ggcctaccca tgttaattct taactttttc tacgctgcgt ataaatcgca tccatcacaa
      721 aaattgccag cgccacccaa ataaaggcga aagtcaccat cttatcggca cccggttttt
      781 caccataaaa cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac tggaaaaagc
      841 ctaacgttga gagacgcaag cgcgtggcag cggcggtaaa acacaacagc ggtacggtag
      901 tgacaatacc ggcggcgatc agcagtaaat tcagcgacat cgggttttgc cccatatggc
      961 tggttgagct gtcggcaata gcaaacaggt aaattgccgc cacgggcagc agccacatgg
     1021 tttcgattaa catgccggtt tgcgcttcaa cggcaatctt cttgcgtacc agaccgtaga
     1081 aggcaaaact aaatgccagt cccagcgcga taataggtag cgaaccaaaa gtccacagct
     1141 ggactaacac gccacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct
     1201 cgccgaggaa aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc
     1261 tcgcttccag catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa
     1321 tcagcacggc agagactgcc agcataaaaa ttttctgtgg cgtctgaatc agcgttttta
     1381 aataggacca ctggcggcaa atgctcatca gcaccaccat aaagaaaaac gaccagatca
     1441 cgcgatgcgt caggatttca tcggcaggca cgtagtaaat caacttgaag tacgctggcg
     1501 ctatacccca aataaaataa gcggcaagag cgagtaatac gccctgccgc gtttgttttg
     1561 catccatcgg gaatactcat ttttaattgg taacagcagt ttacctgctt ttatgtcttc
     1621 aacctaccat ataggtggcg gtggcactgg caatataaag ctgttcttca ttgtgtaatt
     1681 caacgcgggc gacggcgact ttattgcctg cacgcaacag gctactggta gcagtaaaac
     1741 gctcgcccct gcctgggcgc agataatcaa cgcgaagatc aatggtcccc atccgcgata
     1801 gccgctggcg tagttcatct tcactgatgg tttcgtggcg ggttaaggta cttcccacgc
     1861 acaccagacc tgcggcgaca tccagcgctg acgcaataac cccgccgtgc aaaatgcttt
     1921 gcgcccagtt acccaccatc attggctgat ttttaaaggc cagctgtgcg aactcttttt
     1981 cgtaacgctc cagctccatc cctaacgcgc ggttaaatgg catgtgataa acaaacatct
     2041 cacccactaa tttcagggct tgttcagcgg tcagtacggc agacatatca tccttacact
     2101 tcattggtta atgaaatgtt gattttatgc ttctttgttg ttggtttcta ctttaggaag
     2161 ggataactaa cggctatgga gttaagtatg taaaatagcc cgcagaaaaa tattcacctt
     2221 atcaataatt cgttacggag aacacgaccg atgcggactc tgcagggctg gttgttgccg
     2281 gtgtttatgt tgcctatggc agtatatgca caagaggcaa cggtgaaaga ggtgcatgac
     2341 gcgccagcgg tgcgtggcag tattatcgcc aatatgctgc aggagcatga caatccgttc
     2401 acgctctatc cttatgacac caactacctc atttacaccc aaaccagcga tctgaataaa
     2461 gaagcgattg ccagttacga ctgggcggaa aatgcgcgta aggatgaagt aaagtttcag
     2521 ttgagcctgg catttccgct gtggcgtggg attttaggcc cgaactcggt gttgggtgcg
     2581 tcttatacgc aaaaatcctg gtggcaactg tccaatagcg aagagtcttc accgtttcgt
     2641 gaaaccaact acgaaccgca attgttcctc ggttttgcca ccgattaccg ttttgcaggt
     2701 tggacgctgc gcgatgtgga gatggggtat aaccacgact ctaacgggcg ttccgacccg
     2761 acctcccgca gctggaaccg cctttatact cgcctgatgg cagaaaacgg taactggctg
     2821 gtagaagtga agccgtggta tgtggtgggt aatactgacg ataacccgga tatcaccaaa
     2881 tatatgggtt actaccagct taaaatcggc tatcacctcg gtgatgcggt gctcagtgcg
     2941 aaaggacagt acaactggaa caccggctac ggcggcgcgg agttaggctt aagttacccg
     3001 atcaccaaac atgtgcgcct ttatactcag gtttacagcg gctatggcga atcgctcatc
     3061 gactataact tcaaccagac ccgtgtcggt gtgggggtta tgctaaacga tttgttttga
     3121 tgaacggttg agtggttggc aaatctggaa cccagcatcc aggattaccc tctcagagac
     3181 taaaagcatt gcagtttctc gcgcaggcgc tgaaaatagc gcctgttttt atttcaggca
     3241 atcggggtga atgtggcgca ggcggaagtg ttgaatctgg agtccggagc taaacaggtt
     3301 ttacaagaaa cctttggcta ccaacagttt cgccccggcc aggaagaaat tatcgacact
     3361 gtgctttccg gtcgcgattg cctggtcgtc atgcccaccg gtggcggaaa atccctttgc
     3421 tatcaaattc ctgccttatt gctaaacggc cttaccgtgg ttgtttcacc gctgatttcg
     3481 ttgatgaaag atcaggtgga tcaactgcaa gccaacggcg tggcggcggc gtgccttaac
     3541 tcgacgcaaa cccgcgaaca gcaacttgaa gtgatgacag gctgccgcac cgggcaaatt
     3601 cgtctgcttt atatcgcccc ggaacgcctg atgctggata actttcttga gcatctggcg
     3661 cactggaatc cggtgttatt agccgttgat gaagcgcact gtatctccca atggggccac
     3721 gatttccgcc cggaatatgc cgcgctcggt cagttgcgcc agcggttccc gacgctgccg
     3781 tttatggcgc tgaccgccac agccgacgac accacgcgcc aggatatcgt gcgcctgctg
     3841 gggctgaacg atccgctgat tcaaatcagc agttttgacc gtccgaatat tcgctacatg
     3901 ctgatggaga agttcaaacc gctcgatcag ttgatgcgct acgtgcagga acagcgcggt
     3961 aagtccggca ttatctactg caacagccgg gcgaaagtag aagacaccgc tgcgcgcctg
     4021 caaagcaagg gtattagcgc ggcggcctat catgccgggc tggaaaataa tgtccgcgcc
     4081 gacgtgcagg agaaattcca gcgcgatgac ctgcaaattg tggtggcgac ggtggcgttc
     4141 ggcatgggca tcaataaacc aaacgttcgc ttcgtggtcc actttgatat tccgcgcaat
     4201 atcgaatcct attatcagga aaccggtcgc gccgggcgtg atggcctgcc cgcggaagcg
     4261 atgctgtttt acgatccggc cgatatggcg tggctgcgcc gttgtctgga agagaagccg
     4321 caggggcagt tgcaggatat cgaacgtcat aaactcaatg cgatgggcgc ttttgccgaa
     4381 gcgcaaactt gccgtcgtct ggtactgctg aactattttg gcgaagggcg tcaggagccg
     4441 tgcgggaact gcgatatctg cctcgatccg ccgaaacagt acgacggttc aaccgatgct
     4501 cagattgccc tttccaccat tggtcgtgtg aatcagcgtt ttggtatggg ctatgtggtg
     4561 gaagtgattc gcggcgctaa taaccagcgt atccgcgact atggtcatga caaactaaaa
     4621 gtctacggta tgggccgtga taaaagtcat gaacattggg tgagcgtgat ccgccagctg
     4681 attcacctcg gtctggtgac gcaaaatatt gcccagcatt ctgccctaca actgacagag
     4741 gctgcgcgtc cggtgctgcg cggcgaatcc tctttgcaac ttgccgtgcc gcgtatcgtg
     4801 gcgctcaaac cgaaagcgat gcagaaatcg ttcggcggca actatgatcg caaactgttc
     4861 gccaaattac gcaaactgcg taaatcgata gccgatgaaa gtaatgtccc gccgtacgtg
     4921 gtgtttaacg acgcaacctt gattgagatg gctgaacaga tgccgatcac cgccagcgaa
     4981 atgctcagcg ttaacggcgt tgggatgcgc aagctggaac gctttggcaa accgtttatg
     5041 gcgctgattc gtgcgcatgt tgatggcgat gacgaagagt agtcagcagc ataaaaaagt
     5101 gccagtatga agactccgta aacgtttccc ccgcgagtca aatgtatgtt gatgttattt
     5161 ctcaccgtcg ccatggtgca cattgtggcg cttatgagcc ccggtcccga tttctttttt
     5221 gtctctcaga ccgctgtcag tcgttcccgt aaagaagcga tgatgggcgt gctgggcatt
     5281 acctgcggcg taatggtttg ggctgggatt gcgctgcttg gcctgcattt gattatcgaa
     5341 aaaatggcct ggctgcatac gctgattatg gtgggcggtg gcctgtatct ctgctggatg
     5401 ggttaccaga tgctacgcgg tgcactgaaa aaagaggcgg tttctgcacc tgcgccacag
     5461 gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa tctcgctaat
     5521 ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga taacgttggc
     5581 actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc gtggtttacc
     5641 gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg tctggcgaag
     5701 tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt gattatttcg
     5761 cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc gcatccgaca
     5821 ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata aacaacgagc
     5881 cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga gcaatccgtt
     5941 gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg gtgacgccga
     6001 gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga aatagcgccg
     6061 ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc tggaacaaat
     6121 gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg gcgcgccact
     6181 gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact tcaaacgcaa
     6241 tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata tgaatcgcca
     6301 gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg ttgagcgagg
     6361 tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc gatgtcagca
     6421 ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat ttttgtgcca
     6481 caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt ttacgtggtt
     6541 taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag agaaaacgcg
     6601 tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga tgaagcagag
     6661 tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca gcaccatgac
     6721 cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc ggaactggcc
     6781 tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg gcagggacgt
     6841 tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa tgattatgtt
     6901 gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg taaacgctat
     6961 atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg ccatccaggt
     7021 gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg tatgccgtca
     7081 tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg tgatggctat
     7141 gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact gacccacagc
     7201 agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat tcgcgtcggt
     7261 gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt gttagccggt
     7321 gcgggtgatg acgccacgcc aacgcttctc ttacaagctg aagaggaacg cgtggtggat
     7381 aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc tgtcgaagga
     7441 ggacggccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa ggacgcaatg
     7501 cgctcagtcg cgctccacgc catcgttgat tttttcaaca ggcataactc acccagcgga
     7561 aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg atttagatgg
     7621 cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaactc tgaagctgct
     7681 caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg atgtggggca
     7741 aattcgcgat aatctggaga ttaagtctta catgattacc tccaatggtg cgcgcgttca
     7801 cgatctggat ggtaatctga tttttgctca taacctggat cgcgacattg ccagcgatct
     7861 gtttggcgta gtcaacgaca atccggacat cattactaac gtttatcgcg acgacgaatg
     7921 gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg tgttcaaata
     7981 tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt ttttcacctg
     8041 cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgct ggggcgatcg
     8101 cgtcaacgtc agtttctcta ccttaacctg tctggaagtg atggcgggcg gcgtgtcgaa
     8161 aggccatgcg ctggaagcag tggcgaagaa actgggctac agcctgaagg attgtattgc
     8221 gtttggtgac gggatgaacg acgccgaaat gctgtcgatg gcggggaaag gctgcattat
     8281 gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga ttggtactaa
     8341 tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat cgttctttat
     8401 ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca aagagttttc
     8461 cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta gcttttatgg
     8521 cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg ttggcctggc
     8581 ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa cggtcggcct
     8641 gtatatgttg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt tccgcgcgta
     8701 tctctacctg acggtctccg aactgctgct gttcaccgta ctgacgccgc tctacatcac
     8761 gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgcct ttagcgcctt
     8821 gttggcggtg attggtgccg ggattattcg ctatgatcag gtcaccgacc atttctggac
     8881 tggcttgctg ctggtgcaac tctccaatat cacttttgcc atcggcatgg tgggttacaa
     8941 acgcctgatg gaaactcgcc cgatgccaca gcataacgcc tttgcgtggt tctatcttgg
     9001 cgcgtttctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga aaatgccgca
     9061 aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg ggattggcta
     9121 ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca ttatgaataa
     9181 tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac agccgcactg
     9241 gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc atcgtaagtg
     9301 ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg cgctgagcga
     9361 ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc gtacagtcgg
     9421 ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc aaaactctct
     9481 actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg aatcaacaat
     9541 aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag aaaatgccgc
     9601 cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct ggcgagatct
     9661 tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac cagacgcact
     9721 tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc ggaggtcatc
     9781 accagatcca gctcgccctg ttgcaacgcg ggctgcgggt caaacgtcac gcccgattta
     9841 aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg cgtcagccac
     9901 tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg ttcattgcag
     9961 gcttgcagtg cctggctaat ttgcggcagc acctggtttg ccagttgcaa caggatttct
    10021 ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa gccaaggcgt
    10081 tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa cgtcgccgca
    10141 gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg ttttacttcg
    10201 atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat acagtaaccg
    10261 ccaattatgg atgtgtaaac atctggacgg ctaaatacta aaaattccat aaggggcata
    10321 taatgacaat tcttaatcac accctcggtt tccctcgcgt tggcctgcgt cgcgagctga
    10381 aaaaagcgca agaaagttat tgggcgggga actccacgcg tgaagaactg ctgacagtag
    10441 ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca agcgggtatc gacctgctgc
    10501 cggtgggcga ttttgcctgg tacgatcatg ttctgaccac cagtctgctg ctgggtaatg
    10561 ttccgccacg tcatcagaac aaagacggtt cggtagatat cgacaccctg ttccgtattg
    10621 gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc aaatggttta
    10681 acaccaacta tcactacatg gtgccggaat tcgttaaagg ccaacagttc aaactgacct
    10741 ggacgcagct gctggaggaa gtggacgagg cgctggcgct gggccacaac gttaagcctg
    10801 tgctgctggg gccggttact tacctgtggc tggggaaagt gaaaggtgaa cagtttgacc
    10861 gccttagcct gctgaacgac attctgccgg tttatcagca agtgctggca gaactggcga
    10921 aacgcggcat cgagtgggta cagattgatg aacccgcgct ggtactggaa ctaccacagg
    10981 cgtggctgga cgcatacaaa cccgcttacg acgcgctcca gggacaggtg aaactgctgc
    11041 tgaccaccta ttttgaaggc gtaacgccaa atctcgacac gattactgcg ctgcctgttc
    11101 agggtctgca tgttgacctc gtacatggta aagatgacgt tgctgaactg cacaagcgcc
    11161 tgccttctga ctggttgctg tctgcgggtc tgatcaatgg tcgtaacgtc tggcgcgccg
    11221 atcttaccga gaaatatgcg caaattaagg acattgtcgg caaacgtgat ttgtgggtgg
    11281 catcttcctg ctcgttgctg cacagcccca tcgacctgag cgtggaaacg cgtcttgatg
    11341 cagaagtgaa aagctggttt gccttcgccc tacaaaaatg ccatgaactg gcactgctgc
    11401 gcgatgcgct gaacagtggt gacacggcag ctctggcaga gtggagcgcc ccgattcagg
    11461 cacgtcgtca ctctacccgc gtacataatc cggcggtaga aaagcgtctg gcggcgatca
    11521 ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg tgccgaagcc cagcgtgcgc
    11581 gttttaaact gccagcgtgg ccgaccacca cgattggttc tttcccgcaa accacggaaa
    11641 ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga cgccaataac taccgcacgg
    11701 gcattgcgga acatatcagg caggccattg ttgaacagga acgtttggga ctggatgtgc
    11761 tggtacatgg cgaggccgag cgtaatgaca tggtggaata ctttggcgag cacctcgacg
    11821 ggtttgtctt tacgcaaaac ggttgggtac agagctacgg ttcccgctgc gtgaagccac
    11881 cgattgttat tggtgacgtt agtcgcccgg caccgattac cgtggagtgg gcgaagtatg
    11941 cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac ggggccggtg accatactct
    12001 gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag attgcgctgg
    12061 cgctgcgtga tgaggtggcc gatctggaag cggcagggat tggtatcatc cagattgacg
    12121 aaccggcgct gcgcgaaggt ttaccactgc gtcgtagcga ctgggacgcc tatctccagt
    12181 ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa agatgacaca caaatccaca
    12241 ctcacatgtg ttattgcgag ttcaacgaca tcatggattc cattgcggcg ctggatgcag
    12301 acgtcatcac catcgaaacc tcgcgttccg acatggagtt gctggagtcg tttgaagagt
    12361 ttgattatcc gaatgaaatc ggtccaggcg tctatgacat tcactcgcca aacgtaccga
    12421 gcgtggaatg gattgaagcc ctgctgaaga aagcggcaaa acgcattccg gcagagcgcc
    12481 tgtgggtcaa cccagactgt ggcctgaaaa cgcgcggctg gccagaaacc cgcgcggcac
    12541 tggcgaacat ggtgcaggcg gcgcagaatt tgcgtctggg gtaaatccaa accgggtggt
    12601 aataccaccc ggtcttttct cattacagcg acttcttccc accatactgc ttaaaccatt
    12661 ccagcatacg ctgccagcca tctttcgcag attcggcatg atagctcggg cgataatcag
    12721 cgttgaatgc atgcccggca tccgggtaga cgataatctc tgctttcgca ttagcagccc
    12781 gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat gctgttatcc tgaccgccat
    12841 ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat
    12901 tcagcgactt atcgcccgtc agtttgccgt accacgccac tgcggctttt agctgtggat
    12961 tatgcgcggc atacagccag gtgatacgtc caccccagca gaatccggtg atcattaaac
    13021 gatgaacatc gccgccgttg cgggaagccc aactggcgac atgatcgaga tcggccagca
    13081 cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata tcggcaaaat
    13141 cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac ccctccagcg
    13201 ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc tgcactacaa
    13261 tgaccactgg cagtgggcca tcgctttgct ttggtctggc atggtaagca ggcatgttat
    13321 ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccggg gtctgaacga
    13381 tggtcgaagc gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg
    13441 tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc atgcaacaaa
    13501 tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga gtgatttatg
    13561 tgatttgcat cacttttggt gggtaaattt atgcaacgca tttgcgtcat ggtgatgagt
    13621 atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct gactaaaccg
    13681 attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc tcactaaaaa
    13741 cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat
    13801 cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca ccacctggcg
    13861 tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg gcccgtctac
    13921 ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc gtatcggtac
    13981 aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca cggcgtctgt
    14041 ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg tcgctgattt
    14101 cgaatgtacg actgcgctgg ttgaagctgc gaaatccatt ggcgcgacaa ctcacgttgg
    14161 cgtgacagct tcttctgata ccttctaccc aggtcaggaa cgttacgata cttactctgg
    14221 tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg gcgtaatgaa
    14281 ctatgaaatg gaatctgcaa ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg
    14341 tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg ctgagacgat
    14401 gaaacaaacc gaaagccatg cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgta
    14461 attctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg cgtagcgcct
    14521 cgcaggaaat gcctttccaa ctggacgttt gtacagcaca attctatttt gtgcgggtaa
    14581 gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg tttacgcagt tattgcgttg
    14641 gtgggtgtgg caattggctg gctgtttgcc agttatcaac atgcgcagca aaaagccgag
    14701 caattagctg aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca acaaattacc
    14761 caaagcgagc actggcgtgc agagtgcgag ttactcaata acgaagtgcg cagcctgcaa
    14821 agtattaaca cctctctgga ggccgatctg cgtgaagtaa ccacgcggat ggaagccgca
    14881 cagcaacatg ctgacgataa aattcgccag atgattaaca gcgagcagcg cctcagtgag
    14941 cagtttgaaa acctcgccaa ccgtattttt gagcacagca atcgccgggt tgatgagcaa
    15001 aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg aacaactgga cggtttccgc
    15061 cgtcaggttc aggacagctt cggtaaagaa gcacaagaac gccataccct gacccacgaa
    15121 attcgcaatc tccagcaact caacgcgcaa atggcccagg aagcgatcaa cctgacgcgc
    15181 gcgctgaaag gcgacaataa aacccagggc aactggggcg aggtagtatt gacgcgggtg
    15241 ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag catcgaaaat
    15301 gacgcccgct cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg aaaagatgtg
    15361 gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct attttaacgc cgaagacgac
    15421 tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca tatccgtttg
    15481 ctgggacgca aagattatca acagctgccg gggctgcgaa ctctggatta cgtgctgatg
    15541 tttattcccg ttgaacccgc tttttcactg gcgcttgacc gccagccgga gctgatcacc
    15601 gaagcgttga aaaacaatat catgctggtt agcccgacta cgctgctggt ggcgttgcgt
    15661 actattgcca acctgtggcg ttatgagcat caaagccgca acgcccagca aatcgccgat
    15721 cgtgccagca agctgtacga caagatgcgt ttgttcatcg atgacatgtc cgcgattggt
    15781 caaagtctcg acaaagcgca ggataactac cgccaggcaa tgaaaaaact ctcttcaggt
    15841 cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt taggagtaga aattaaacgc
    15901 gagattaatc cggatttggc tgaacaggcg gtgagccagg atgaagagta tcgacttcgg
    15961 tcggttccgg agcagccgaa tgatgaagct tatcaacgcg atgatgaata taatcagcag
    16021 tcgcgctagc ccattagggg tagttaagcc gggtagaaat ctagggcatc gacgcccaat
    16081 ctgttacact tctggaacaa ttttttgatg agcaggcatt gagatggtgg ataagtcaca
    16141 agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc
    16201 ccacgttttc cattccgtgg catcaaaata cgatgtcatg aatgatttga tgtcatttgg
    16261 tattcatcgt ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc gtgggcagac
    16321 cgtgctggat ctggctggtg gcaccggcga cctgacagcg aaattctccc gcctggtcgg
    16381 agaaactggc aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa tgggacgcga
    16441 gaagctgcgt aatatcggtg tgattggcaa cgttgagtat gttcaggcga acgctgaggc
    16501 gctgccgttc ccggataaca cctttgattg catcaccatt tcgtttggtc tgcgtaacgt
    16561 caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct
    16621 gctggtgctt gagttctcga agccaattat cgagccgctg agcaaagcct atgatgcata
    16681 ctccttccat gtgctgccgc gtattggctc actggtcgcg aacgacgccg acagctaccg
    16741 ttatctggca gaatccatcc gtatgcatcc cgatcaggat accctgaaag ccatgatgca
    16801 ggatgccgga ttcgaaagtg tcgactacta caatctgacg gcgggggttg tggcgctgca
    16861 tcgtggttat aagttctgac aggagaccgg aaatgccttt taaaccttta gtgacggcag
    16921 gaattgaaag tctgctcaac accttcctgt atcgctcacc cgcgctgaaa acagcccgct
    16981 cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg cttttcgacg tcattgattc
    17041 tggtgttcag cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat gctgactgca
    17101 ccgttatcgc ctacgccagt gtgttgccga aacttcgcga tcgccagcag cttaccgcac
    17161 tgattcgcag tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa aacttcgttg
    17221 cgctggcaga tctggcagag ttcgaccctg cggaactgct ggccccttat accggtgata
    17281 tcgccgctga aggaatcagc aaagccatgc gcggaggcgc aaagttcctg catcacggca
    17341 ttaagcgcca gcaacgttat gtggcggaag ccattactga agagtggcgt atggcacccg
    17401 gtccgcttga agtggcctgg tttgcggaag agacggctgc cgtcgagcgt gctgttgatg
    17461 ccctgaccaa acggctggaa aaactggagg ctaaatgacg ccaggtgaag tacggcgcct
    17521 atatttcatc attcgcactt ttttaagcta cggacttgat gaactgatcc ccaaaatgcg
    17581 tatcaccctg ccgctacggc tatggcgata ctcattattc tggatgccaa atcggcataa
    17641 agacaaactt ttaggtgagc gactacgact ggccctgcaa gaactggggc cggtttggat
    17701 caagttcggg caaatgttat caacccgccg cgatcttttt ccaccgcata ttgccgatca
    17761 gctggcgtta ttgcaggaca aagttgctcc gtttgatggc aagctggcga agcagcagat
    17821 tgaagctgca atgggcggct tgccggtaga agcgtggttt gacgattttg aaatcaagcc
    17881 gctggcttct gcttctatcg cccaggttca taccgcgcga ttgaaatcga atggtaaaga
    17941 ggtggtgatt aaagtcatcc gcccggatat tttgccggtc attaaagcgg atctgaaact
    18001 tatctaccgt ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc gtctgcgccc
    18061 aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat gaactgaatt tgctgcggga
    18121 atctgccaac gccattcagc ttcggcgcaa ttttgaagac agcccgatgc tctacatccc
    18181 ggaagtttac cctgactatt gtagtgaagg gatgatggtg atggagcgca tttacggcat
    18241 tccggtgtct gatgttgcgg cgctggagaa aaacggcact aacatgaaat tgctggcgga
    18301 acgcggcgtg caggtgttct tcactcaggt ctttcgcgac agctttttcc atgccgatat
    18361 gcaccctggc aacatcttcg taagctatga acacccggaa aacccgaaat atatcggcat
    18421 tgattgcggg attgttggct cgctaaacaa agaagataaa cgctatctgg cagaaaactt
    18481 tatcgccttc tttaatcgcg actatcgcaa agtggcagag ctacacgtcg attcaggctg
    18541 ggtgccacca gataccaacg ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc
    18601 tatctttgag aaaccgctgg ccgaaatttc gtttggacat gtactgttaa atctgtttaa
    18661 tacggcgcgt cgcttcaata tggaagtgca gccgcaactg gtgttactcc agaaaaccct
    18721 gctctatgtc gaaggggtag gacgccagct ttatccgcag ctcgatttat ggaaaacagc
    18781 gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt attcctgcgc tggtgagagc
    18841 atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg aattggttta
    18901 cgacagtttg cgccagggca agtatttaca gcacagtgtt gataagattg cccgcgagct
    18961 tcagtcaaat catgtacgtc agggacaatc gcgttatttt ctcggaattg gcgctacgtt
    19021 agtattaagt ggcacattct tgttggtcag ccgacctgag tgggggctga tgcccggctg
    19081 gttaatggca ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt
    19141 tcatcgctca aggcgggccg tgtaacgtat aatgcggctt tgtttaatca tcatctacca
    19201 cagaggaaca tgtatgggtg gtatcagtat ttggcagtta ttgattattg ccgtcatcgt
    19261 tgtactgctt tttggcacca aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat
    19321 caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa ccagtcagga
    19381 tgctgatttt actgcgaaaa ctatcgccga taagcaggcg gatacgaatc aggaacaggc
    19441 taaaacagaa gacgcgaagc gccacgataa agagcaggtg taatccgtgt ttgatatcgg
    19501 ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc gttctggggc cgcaacgact
    19561 gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac
    19621 ggtgcagaac gaactgaccc aggagttaaa actccaggag tttcaggaca gtctgaaaaa
    19681 ggttgaaaag gcgagcctca ctaacctgac gcccgaactg aaagcgtcga tggatgaact
    19741 acgccaggcc gcggagtcga tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag
    19801 cgatgaagcg cacaccatcc ataacccggt ggtgaaagat aatgaagctg cgcatgaggg
    19861 cgtaacgcct gccgctgcac aaacgcaggc cagttcgccg gaacagaagc cagaaaccac
    19921 gccagagccg gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc
    19981 ttcgtcgagt gataaaccgt aaacatgtct gtagaagata ctcaaccgct tatcacgcat
    20041 ctgattgagc tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt gatattcctg
    20101 tgtctggtct atttcgccaa tgatatctat cacctggtat ccgcgccatt gatcaagcag
    20161 ttgccgcaag gttcaacgat gatcgccacc gacgtggcct cgccgttctt tacgccgatc
    20221 aagctgacct ttatggtgtc gctgattctg tcagcgccgg tgattctcta tcaggtgtgg
    20281 gcatttatcg ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt
    20341 tccagctctc tgctgtttta tatcggtatg gcattcgcct actttgtggt ctttccgctg
    20401 gcatttggct tccttgccaa taccgcgccg gaaggggtgc aggtatccac cgacatcgcc
    20461 agctatttaa gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg
    20521 gtggcaattg tgctgctgtg ctggatgggg attacctcgc cagaagactt acgcaaaaaa
    20581 cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc
    20641 ttctcgcaaa cgctattggc gatcccgatg tactgtctgt ttgaaatcgg tgtcttcttc
    20701 tcacgctttt acgttggtaa agggcgaaat cgggaagagg aaaacgacgc tgaagcagaa
    20761 agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg cggttgtcat atggagtaca
    20821 ggatgtttga tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg
    20881 ttgtagcgcg cgcttttgac gcgggagtta atgggctact catcaccggc actaacctgc
    20941 gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg
    21001 gcgtacatcc tcacgacagc agccagtggc aagctgcgac tgaagaagcg attattgagc
    21061 tggccgcgca gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact
    21121 tttcgacgcc ggaagagcag gaacgcgctt ttgttgctca gctacgcatt gccgcagaat
    21181 taaacatgcc ggtatttatg cactgtcgcg atgcccacga gcggtttatg acattgctgg
    21241 agccgtggct ggataaactg cctggtgcgg ttcttcattg ctttaccggc acacgcgaag
    21301 agatgcaggc gtgcgtggcg cgtggaattt atatcggcat taccggttgg gtttgcgatg
    21361 aacgacgcgg gctggagctg cgggaattgt tgccgttgat tccggcggag aaattgctga
    21421 tcgaaactga tgcgccgtat ctgctcccgc gcgatctcac gccaaagcca tcatcccggc
    21481 gcaacgagcc agcccatctg ccccatattt tgcaacgtat tgcgcactgg cgtggagaag
    21541 atgccgcatg gctggctgcc accacggatg ctaatgtcaa aacactgttt gggattgcgt
    21601 tttagagttt gcggaactcg gtgttcttca cactgtgctt aatctcttta ttaataagat
    21661 taagcaatag catggagcga gcctcaccat cgggttcggt gaaaatggcc tgaaagcctt
    21721 cgaacgcgcc ttcggtaata atcaccttat cacccggata aggggttgcc ggatcgacaa
    21781 tgtctttcgg tttatatacc gatagctgat gaatcaccgc cgatgggact atcgctggcg
    21841 acgcgccaaa gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc gtggtatgaa
    21901 tcacttctgg atcaaattcg acaaacaggt agttgggaaa caatggctca ctgactgcag
    21961 tacgttttcc acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct
    22021 gtctttcgag gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc
    22081 aggattgcat aatgactctt atccgtttaa tcggggcgca aggatagcaa aagctttacg
    22141 ctaagttaat tatattcccc ggtttgcgtt ataccgtcag agttcacgct aatttaacaa
    22201 atttacagca tcgcaaagat gaacgccgta taatgggcgt agattaagag gctacaatgg
    22261 acgccatgaa atataacgat ttacgcgact tcttgacgct gcttgaacag cagggtgagc
    22321 taaaacgtat cacgctcccg gtggatccgc atctggaaat cactgaaatt gctgaccgca
    22381 ctttgcgtgc cggtgggcct gcgctgttgt tcgaaaaccc taaaggctac tcaatgccgg
    22441 tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat gggcatgggg caggaagatg
    22501 tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct gaaagagccg gagccgccaa
    22561 aaggtttccg cgacctgttt gataaactgc cgcagtttaa gcaagtattg aacatgccga
    22621 caaagcggct gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat gacgtcgatc
    22681 tcaatcgcat tcccattatg acctgctggc cggaagatgc cgcgccgctg attacctggg
    22741 ggctgacagt gacgcgcggc ccgcataaag agcggcagaa tctgggcatt tatcgccagc
    22801 agctgattgg taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc ggcgcgctgg
    22861 attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg
    22921 gtgccgatcc cgccacgatt ctcggtgcag tcacccccgt tccggataca ctttcagagt
    22981 atgcgtttgc cggattgctg cgcggtacca agaccgaagt ggtgaagtgt atctccaatg
    23041 accttgaagt gcccgccagt gcggagattg tgctggaagg gtatatcgaa caaggtgaaa
    23101 cggcgccgga agggccgtat ggcgaccaca ccggttacta taacgaagtc gatagtttcc
    23161 cggtatttac cgtgacgcat attacccagc gtgaagatgc gatttaccat tccacctata
    23221 ccgggcgtcc gccagatgag cccgcggtac tgggcgtggc gttgaacgaa gtgtttgtac
    23281 cgattctgca aaaacagttc ccggaaattg tcgattttta cctgccgccg gaaggctgtt
    23341 cttatcgtct ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg aagcgcgtca
    23401 tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac taaatttgtg atcgtttgcg
    23461 atgatgacgt caacgcacgc gactggaacg atgtgatttg ggcgattacc acccgtatgg
    23521 acccggcgcg ggatactgtt ctggtagaaa atacgcctat tgattatctg gattttgcct
    23581 cgcctgtctc cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa tggccggggg
    23641 aaacccagcg tgaatgggga cgtcccatca aaaaagatcc agatgttgtc gcgcatattg
    23701 acgccatctg ggatgaactg gctattttta acaacggtaa aagcgcctga tgcgcgtttg
    23761 ttttgcccta tttatcgatc cgacagagaa agcgcatgac aaccttaagc tgtaaagtga
    23821 cctcggtaga agctatcacg gataccgtat atcgtgtccg catcgtgcca gacgcggcct
    23881 tttcttttcg tgctggtcag tatttgatgg tagtgatgga tgagcgcgac aaacgtccgt
    23941 tctcaatggc ctcgacgccg gatgaaaaag ggtttatcga gctgcatatt ggcgcttctg
    24001 aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct caaagatcat caaatcgtgg
    24061 tcgatattcc ccacggagaa gcgtggctgc gcgatgatga agagcgtccg atgattttga
    24121 ttgcgggcgg caccgggttc tcttatgccc gctcgatttt gctgacagcg ttggcgcgta
    24181 acccaaaccg tgatatcacc atttactggg gcgggcgtga agagcagcat ctgtatgatc
    24241 tctgcgagct tgaggcgctt tcgttaaagc accctggtct gcaagtggtg ccggtggttg
    24301 aacaaccgga agcaggctgg cgtgggcgta ctggtaccgt gttaacggcg gtattgcagg
    24361 atcatggtac gctggcagag catgatatct atattgccgg acgttttgag atggcgaaaa
    24421 ttgcccgtga cctgttttgc agtgagcgta atgcgcggga agatcgcctg tttggcgatg
    24481 cgtttgcatt tatctgagat ataaaaaaac ccgcccctga caggcgggaa gaacggcaac
    24541 taaaacttta ttcagtggca tttagatcta tgtcgcatcc ggcaatcggt gcacgatgcc
    24601 tgatgcgacg ctggcgcgtc ttatcaggcc tacattggtg caggatcggt agaccggata
    24661 aggcgttcac gccgcatccg gcaagtggtt aaacccgctc aaacaccgtc gcaataccct
    24721 gacccagacc gatacacatc gtcgccagac caaactgaac gtctttgcgt tccatcagat
    24781 tcagcagcgt ggtgctgata cgcgcaccgg aacagcccag cggatgaccc agcgcgatcg
    24841 caccaccgtt gaggttgatc ttctcgtcaa tctgctcaat cagtcccaga tctttaatac
    24901 atggcaggat ctgcgcggca aaggcttcgt tcatctcaaa cacgccgata tcgctggcag
    24961 aaagccccgc ttttttcagc gccagcttcg aggccggaac cgggccgtaa cccataatcg
    25021 atgggtcaca accaacgacc gccatcgaac gcacacgagc gcgcggctta agacctaatt
    25081 catgggcgcg gctttcactc atcaccagca tggcagctgc gccatcggaa agtgcagaag
    25141 atgtgcccgc cgttaccgta ccgtttactg gatcaaacgc cggacgcagc gtggcgaggg
    25201 cttccacggt ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc aggacgccgt
    25261 cggcatcgtg accaccagtt gggatgattt catttttaaa tgcggccgac tgcgtggcgg
    25321 cccaggcgcg ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg ctgataccgt
    25381 gcatacgcgc cagcatttct gctgttaagc ccatcatgcc cgccgctttg gcgacattgc
    25441 ggctcaggcc ggggtgaaaa tcgacgccgt gactcatcgg cacatggccc atatgctcca
    25501 cgccgccaac cagacatgcc tgcgcatcgc cggtcataat catccgtgct gcgtcatgca
    25561 gtgcctgcat ggatgaacca cacaagcgat tgacggtaac cgccgggaca gagtgtggta
    25621 cttctgccag cagcgccgcg ttacgggcga tgttaaaacc ctgctccagc gtctgctgca
    25681 cacaacccca gtaaatatcg tcgagggccg ccgcttccag cgccgggtta cgcgccagca
    25741 ggctacgcat taaatgagcg gagagatctt ctgcacgcac gttacgaaaa gcaccgccct
    25801 tcgaacggcc catcggggtg cgaattgcat cgacaatgac aacctgttcc attgtgactc
    25861 cttaagccgt tttcaggtcg ccaaccggac gggctggctc aaccggagga tagtacggtt
    25921 cgttatgacg cgctttatta cgcagacctt ccggcacttc atacagcggg ccgaggtgct
    25981 gatattgctg tgccatatcg aggtattttg cgctaccgag ggtgtccagc cagcggaacg
    26041 cgccgccgtg gaacggaggg aagcccaggc cgtagaccag cgccatatcc gcttccgccg
    26101 gagtggcgat aatgccttcc tccagacagc gcaccacttc gttgaccatc gggatcatca
    26161 tgcgggcgat aatctcctct tcgctgaaat cgcgcttcgg ctggctcact tctgccagca
    26221 ggtcttcaac ggcggcgtct tcttctttct tcggcttacc tttgctgtct tctttataac
    26281 gccagaaacc gaggccgttc ttctgaccaa agcggttggc atcaaacagc gcgtcgatgg
    26341 catcgcggta atctttctgc atcctctgcg ggaagcctgc tgccatgaca gcctgggcat
    26401 gatgcgcggt gtcaatgccc acgacatcca gcagatatgc cggacccatc ggccagccaa
    26461 actgtttttc catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc agcagctggc
    26521 tgaaaccggc gaaatacggg aacagcacgc ggttaacaaa gaagccgggg cagtcgttaa
    26581 ccacaatcgg cgtcttgccc atcttgctcg cccaggcgac aactttcgcg atggtttcgt
    26641 cggagctttt ctcgccgcga ataatttcta ccaacggcat tcggtggacc gggttaaaga
    26701 agtgcatccc gcagaagttt tccgggcgtt tcagcgcgtt ggccagttcg ctgataggaa
    26761 tggttgaagt gttagacgcc agcacggtat cctggcgtac tttttgttcg gtttctgcca
    26821 gtacggcttt tttcactttc gggttttcaa caaccgcttc taccacaata tccacgcggt
    26881 caaatccggc gtagtcgagc gttgggtgga ttgtggagat cacgccagcc agtttcagac
    26941 catcgatctt gccgcgctca agctgcttgt tcagcagttt cgcggcttcg gtcatgccga
    27001 gggttaacga cttgtcgttg atatctttca tgacaaccgg cacgcctttc cacgcagact
    27061 ggtaagcgat gccgccgccc ataatgcctg cacccagcac cgcggcctgt ttcggggttt
    27121 caacgtcttt ggtgagtttc ttcgctttgc cttttacata ttgatcgtta aggaaaatgc
    27181 cgaccagtgc gcgggcttcg ttggtatgcg ccagcgggac aaaacttttg ttttccaggt
    27241 ttaaggcttc ttcacgacca aaacgggccg cagcttcaat ggtttttact gcggtgatgg
    27301 gggccggata atgtttcccc gctgtttgtg cgaccatccc tttagcgatg gtgaagctca
    27361 tggtggcttc aatcttgctc agttttagtg gttccagctt cggctgacgt tttgctttcc
    27421 agtcgaggtc gccgttaatg gcctggcgta aaaccgcctt tgcgccttca accagttttt
    27481 ctgctttgac tacgccatcc accagaccga ttttcagcgc ctgatccgcg ccgacatctt
    27541 taccggcggc aatgatttcc agcgcactgt ctgcgcccag catacgtggc atacgtacag
    27601 aaccgccaaa gcccggcatg atgcccagtt tggtttccgg cagaccgatg cgcagatccg
    27661 gcgtcgccag acgataatcg gtcgccagca cgcattcgca gccaccgccc agcgcatagc
    27721 cgttgacggc agcaatggtc ggcaccggca gatcttccag gcgattaaac acgctattgg
    27781 caaagtgcag ccactgactt aactgttctt caggaacgag gaacagggac aaaaattcgg
    27841 tgatatcagc accgacgata aaggctgctt tgttcgaacg cagcagcagc ccttttagat
    27901 ctgattgctg ttccagcacg ccgatggcct cgccgaggct ggcgacggtc gcagtgtcga
    27961 gtttattaac tgaacctggg gcatcaaata ccagttcggc aatgccatct tccagccagt
    28021 caaggtacag ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc aaggtgatct
    28081 ggtcgtacca gatgcatcga agtgtgtatt ttgtgttaaa aatatgcaaa taaaagatta
    28141 aagaaatgcc gatctgatca cgctcggcag aaatcacgct ctggatgaac gatgtgctaa
    28201 gatgcggaga cttaaggtca aaaaaacaga agggtaaaaa atggaatcac tggcctcact
    28261 ctataaaaat catatagcta ccttacagga acggactcgc gatgcgctgg cgcgcttcaa
    28321 gctggatgcg ttacttattc actccggcga actgttcaac gtttttctcg acgatcatcc
    28381 ctatccgttt aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc aggtgccaaa
    28441 ctgctggctg ctggtggatg gcgtgaacaa gccgaaactg tggttctatc tgccggttga
    28501 ttactggcac aacgtcgaac cgctgccgac ctctttctgg actgaagacg tggaagtgat
    28561 cgcgctgccg aaagccgatg gcattggtag cctgctgcct gctgcgcgcg ggaatatcgg
    28621 ttatatcggt ccggtgccgg aacgtgcgct gcaactgggt attgaggcca gcaatatcaa
    28681 cccgaaaggg gtgatcgact acctgcatta ctaccgctcc ttcaaaaccg agtacgaact
    28741 ggcctgtatg cgtgaagcgc agaaaatggc ggtcaacggt catcgcgcgg cagaagaagc
    28801 gttccgttct ggcatgagcg agttcgatat caacattgcc tatctgaccg cgaccggtca
    28861 tcgtgatacc gacgtacctt acagcaacat tgtggcgctc aacgaacacg ctgcggtgtt
    28921 gcattacacc aaactggacc accaggcacc ggaagagatg cgcagcttcc tgctggatgc
    28981 cggggccgaa tataacggct atgccgctga cctgacccgt acctggtcgg caaaaagtga
    29041 caacgattac gcacagctgg tgaaagacgt aaatgatgaa caactggcgc tgatcgcgac
    29101 catgaaagct ggcgttagct atgtggatta ccacatccag ttccatcagc gcatcgccaa
    29161 actgctgcgt aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg tcgaaaacga
    29221 tctcaccgga ccgtttatgc cgcacggtat cggccatccg ctgggcctgc aggtgcatga
    29281 cgtcgccggt tttatgcagg atgatagcgg tacgcacctc gcggcaccgg caaaatatcc
    29341 gtacctgcgc tgcacccgta ttctccagcc gggcatggtg ttaaccatcg aaccgggtat
    29401 ctacttcatt gaatcgctac tggcaccgtg gcgtgaaggg cagttcagca agcacttcaa
    29461 ctggcagaaa attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag acaacgtggt
    29521 gatccacgaa aataacgtgg aaaacatgac ccgggatctg aaactggcgt gatggaaagc
    29581 tggttaattc ctgcggcacc ggtcacggtc gttgaagaga tcaaaaagag ccgcttcata
    29641 acgatgttgg cgcataccga tggcgttgag gcggcgaaag cgtttgttga atcggtgcgg
    29701 gcagaacacc ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc gccggatgat
    29761 tcacaacagc tgggtttctc tgacgacggg gagccggcgg gaacggcagg taaaccgatg
    29821 ctcgcccagc taatgggcag cggcgttggg gaaattaccg ctgtggtggt gcgctactac
    29881 ggcggcatat tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg cgtgaatcag
    29941 gcgctgcgcc agctaacgac ccaacgcaag acgccattaa ccgaatatac tttgcaatgt
    30001 gaatatcatc agttaaccgg cattgaagcg ttgctggggc agtgtgacgg caaaattatc
    30061 aacagtgatt atcaggcatt cgttctgctg cgggtggcgc ttccggcggc gaaagtggct
    30121 gaattttccg caaagctggc ggattttagc cgtggttcat tgcaattgtt agcgattgaa
    30181 gaataatccc cacttcgttt tgcagaacta aggaagcggc agagatgcat tttcgcgcca
    30241 ttacccgaat cgttggacta ctggtcatct tattttcagg gaccatgatt atccctgggc
    30301 tggtagcact catctaccgg gatggagcgg gccgcgcttt tacccagacc ttttttgtcg
    30361 ccctcgccat tggctctatg ctgtggtggc cgaaccgcaa agagaaaggc gaacttaaat
    30421 cccgtgaggg gtttctgata gtggtgctgt tctggaccgt gctgggtagc gtcggtgcgc
    30481 tcccttttat cttctcggaa agcccgaacc tcacgattac cgatgcgttt tttgaatctt
    30541 tctctggcct gaccaccacc ggggccacta cgctggtggg gctggattcg ctccctcacg
    30601 ccatcctctt ttatcgccag atgctgcaat ggtttggcgg gatggggatc atcgtgttag
    30661 cggttgcgat actgcctatc ctcggcgtgg gtgggatgca gctctatcgc gcagaaatgc
    30721 ccggcccgct gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg aaaaccctgt
    30781 ggttgattta tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt gccggaatgg
    30841 atgcctttga tgccatcggc catagctttg cgactatcgc tattggcggc ttctcgacac
    30901 atgatgccag tatcggttat ttcgatagcc cgactattaa cactatcatt gctatcttcc
    30961 tgctgatctc cggctgtaac tacggtctgc acttttcact gttaagtggg cgtagtctga
    31021 aggtttattg gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt acgctggtgg
    31081 ttatttgtac cctcgtactg tggtttcata atgtctacag ttcggcgctg atgacaatta
    31141 accaggcgtt tttccaggtg gtgtcgatgg cgacaaccgc cgggtttacg actgacagca
    31201 ttgcccgctg gccgctcttt ttgccggtac tgcttttatg ttcggctttt atcggcggtt
    31261 gtgccgggtc aacgggcggt ggcctgaaag tgatccgcat cctgctgctg tttaagcagg
    31321 ggaaccgtga gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt aagctgggga
    31381 atcgcgcact gccggaacgt atcctcgaag ccgtttgggg atttttctcc gcctatgcat
    31441 tggtgtttat tgtcagtatg ctggcgatta tcgccacggg cgtggatgac ttttctgcct
    31501 ttgcctcagt tgttgcgaca ttgaataacc tggggccagg gcttggcgtg gttgctgata
    31561 actttaccag tatgaacccg gtggctaaat ggatcctgat tgccaacatg ctgtttggtc
    31621 gtctcgaggt ctttacattg ctggtgctct ttaccccgac tttctggcgt gaatgatgga
    31681 gtaatacgtg aaaacattaa ttcttttctc aacaagggac ggacaaacgc gcgagattgc
    31741 ctcctacctg gcttcggaac tgaaagaact ggggatccag acggatgtcg ccaatgtgca
    31801 ccgcattgaa gaaccacaat gggaaaatta tgaccgtgtg gtcattggtg cttctattcg
    31861 ctatggtcac taccactctg cgttccagga atttgtcaaa aaacatgcga cgcgactgaa
    31921 ttcgatgccg agcgcctttt attccgtaaa cctggtggcg cgcaaaccgg agaagcgtac
    31981 tccacagacc aacagctacg cgcggaagtt tctgatgaac tcgcaatggc gtcccgatcg
    32041 ctgcgcggtc attgccgggg cgctgcgtta cccacgttat cgctggtacg accgttttat
    32101 gatcaagctg attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag aagttgtcta
    32161 taccgattgg gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa ccgacaaacc
    32221 gacgctgaaa taagcacaaa gaa
//
LOCUS       DAZKGS010000040        32052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKGS010000040 DAZKGS010000000
VERSION     DAZKGS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            273..2978
                     /gene="malT"
                     /locus_tag="ACHOQY_001807"
     CDS             273..2978
                     /gene="malT"
                     /locus_tag="ACHOQY_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9677925.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3021..4037)
                     /gene="rtcA"
                     /locus_tag="ACHOQY_001808"
     CDS             complement(3021..4037)
                     /gene="rtcA"
                     /locus_tag="ACHOQY_001808"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9677926.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4041..5267)
                     /gene="rtcB"
                     /locus_tag="ACHOQY_001809"
     CDS             complement(4041..5267)
                     /gene="rtcB"
                     /locus_tag="ACHOQY_001809"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9677927.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5456..7054
                     /gene="rtcR"
                     /locus_tag="ACHOQY_001810"
     CDS             5456..7054
                     /gene="rtcR"
                     /locus_tag="ACHOQY_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9677928.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(7036..7794)
                     /locus_tag="ACHOQY_001811"
     CDS             complement(7036..7794)
                     /locus_tag="ACHOQY_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9677929.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7811..8641)
                     /gene="glpG"
                     /locus_tag="ACHOQY_001812"
     CDS             complement(7811..8641)
                     /gene="glpG"
                     /locus_tag="ACHOQY_001812"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9677930.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8686..9012)
                     /gene="glpE"
                     /locus_tag="ACHOQY_001813"
     CDS             complement(8686..9012)
                     /gene="glpE"
                     /locus_tag="ACHOQY_001813"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9677931.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9202..10707
                     /gene="glpD"
                     /locus_tag="ACHOQY_001814"
     CDS             9202..10707
                     /gene="glpD"
                     /locus_tag="ACHOQY_001814"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9677932.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSRQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10759..11364)
                     /locus_tag="ACHOQY_001815"
     CDS             complement(10759..11364)
                     /locus_tag="ACHOQY_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677933.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(11367..12134)
                     /locus_tag="ACHOQY_001816"
     CDS             complement(11367..12134)
                     /locus_tag="ACHOQY_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677934.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(12137..12847)
                     /locus_tag="ACHOQY_001817"
     CDS             complement(12137..12847)
                     /locus_tag="ACHOQY_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677935.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(12863..14371)
                     /locus_tag="ACHOQY_001818"
     CDS             complement(12863..14371)
                     /locus_tag="ACHOQY_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9677936.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(14497..16944)
                     /gene="glgP"
                     /locus_tag="ACHOQY_001819"
     CDS             complement(14497..16944)
                     /gene="glgP"
                     /locus_tag="ACHOQY_001819"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9677937.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(16963..18396)
                     /gene="glgA"
                     /locus_tag="ACHOQY_001820"
     CDS             complement(16963..18396)
                     /gene="glgA"
                     /locus_tag="ACHOQY_001820"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9677938.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(18396..19691)
                     /gene="glgC"
                     /locus_tag="ACHOQY_001821"
     CDS             complement(18396..19691)
                     /gene="glgC"
                     /locus_tag="ACHOQY_001821"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9677939.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(19709..21682)
                     /gene="glgX"
                     /locus_tag="ACHOQY_001822"
     CDS             complement(19709..21682)
                     /gene="glgX"
                     /locus_tag="ACHOQY_001822"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9677940.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(21679..23865)
                     /gene="glgB"
                     /locus_tag="ACHOQY_001823"
     CDS             complement(21679..23865)
                     /gene="glgB"
                     /locus_tag="ACHOQY_001823"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9677941.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24138..25241)
                     /gene="asd"
                     /locus_tag="ACHOQY_001824"
     CDS             complement(24138..25241)
                     /gene="asd"
                     /locus_tag="ACHOQY_001824"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9677942.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            25433..26026
                     /gene="yhgN"
                     /locus_tag="ACHOQY_001825"
     CDS             25433..26026
                     /gene="yhgN"
                     /locus_tag="ACHOQY_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9677943.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26083..27423)
                     /gene="gntU"
                     /locus_tag="ACHOQY_001826"
     CDS             complement(26083..27423)
                     /gene="gntU"
                     /locus_tag="ACHOQY_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9677944.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(27427..27954)
                     /gene="gntK"
                     /locus_tag="ACHOQY_001827"
     CDS             complement(27427..27954)
                     /gene="gntK"
                     /locus_tag="ACHOQY_001827"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9677945.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(28093..29088)
                     /gene="gntR"
                     /locus_tag="ACHOQY_001828"
     CDS             complement(28093..29088)
                     /gene="gntR"
                     /locus_tag="ACHOQY_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9677946.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(29312..30007)
                     /gene="yhhW"
                     /locus_tag="ACHOQY_001829"
     CDS             complement(29312..30007)
                     /gene="yhhW"
                     /locus_tag="ACHOQY_001829"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9677947.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(30130..31167)
                     /gene="yhhX"
                     /locus_tag="ACHOQY_001830"
     CDS             complement(30130..31167)
                     /gene="yhhX"
                     /locus_tag="ACHOQY_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9677948.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            31500..31988
                     /gene="yhhY"
                     /locus_tag="ACHOQY_001831"
     CDS             31500..31988
                     /gene="yhhY"
                     /locus_tag="ACHOQY_001831"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9677949.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         7927 a         8648 c         7999 g         7478 t
ORIGIN      
        1 tctttttaga tggcactttc atcaggaatg aagaagaaac ccttgcttaa atgaatctga
       61 tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg agttgttaat
      121 aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt catcgcttgc
      181 attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa aaaaataata
      241 tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa ctaagtcgtc
      301 cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt tccggcgcga
      361 acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc accctcattt
      421 cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa ggtgataacc
      481 agcaagagcg tttcgccagc tatctcattg ccgccgtgca gcaggcaacc aacggtcact
      541 gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg
      601 cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc atcgatgact
      661 atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt cgccatcaac
      721 cagaaaatct caccctggtg gtgttgtcac gcaaccttcc gcaactgggc attgccaatc
      781 tgcgtgttcg tgatcaactg ctggaaattg gcagtcagca actggcattt acccatcagg
      841 aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagctgca gaaagcagtc
      901 ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc ctctccgccc
      961 ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc aatgccagcc
     1021 atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca acgcgccatt
     1081 ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc cgtgtgaccg
     1141 gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg tttttacagc
     1201 ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac ttcctgcgcc
     1261 agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa
     1321 gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg gcaggcgatg
     1381 cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac catagcgaac
     1441 tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg gaaaatccgc
     1501 agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc gaagttaaca
     1561 ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc atgcacgcag
     1621 aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatccggat gaagcggaac
     1681 ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc cgcattgtgg
     1741 caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc tcactggcgc
     1801 taatgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac gccttatgga
     1861 gtttaatcca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc gcgtgggaaa
     1921 cacaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg ccgatgcatg
     1981 agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg gatgaagccg
     2041 aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag caacagcttc
     2101 agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat aacgcccgta
     2161 gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc gactggatct
     2221 ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa gccgccgctg
     2281 ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc
     2341 aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct gcggaaattg
     2401 ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta
     2461 acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac gcccagcgcg
     2521 tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagccat tttgtcatcg
     2581 aaggcgaagc gatggcgcaa caactgcgcc agctgattca actcaatacg ctgccggaac
     2641 tggaacagca tcgcgcacag cgtattctgc gagaaatcaa tcaacatcat cggcataaat
     2701 tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac
     2761 tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg atttactccg
     2821 gttacagcaa tgaacaaatt gcgggcgaac tggaagtggc ggcaaccacc atcaagacgc
     2881 atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc gtgcagcacg
     2941 cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttagccggat aatgcattgg
     3001 atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc tgtttctatc
     3061 aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga tattggtcag cagatggcat
     3121 gaaggatggg cgaccttaaa ttctcccgcg cccgccagcg ccatcggcag taccagttgg
     3181 tcggcgagat attcccccac cgccgctggg cttgccaggt agcgtttcac ctctttcact
     3241 aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacgaa aaagcgttca
     3301 gtgatatttt cactttcgac ttccagcgag acggtattac ccggcccctg gtcgcgcggc
     3361 aggttatgaa tattctgttc atgcagggaa aaactccccg ccagtgtagc gatttcacgc
     3421 tcagcaacat ggcgcggcac accagctaat agaacttctc cacgcatctg cacaatgttc
     3481 ccgcgctcgc caagttgcaa ggtgttaaac gatgccacgg gcgagacttc cgttgccacc
     3541 acgccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg atgaattcct
     3601 attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg ggccgacggg
     3661 ttatcggtgc ctccgctcac ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc
     3721 agcaccgttt gcagcaccag cgtacagctt ccggcactac cgatagcaaa gcggtactcg
     3781 ccgccgcgca cggtgccagg ccggaagacc agacgctgcg accccagctc cgcgccttcc
     3841 accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgctg gcgcaacagc
     3901 cccggtttcg cccgcccggc acgaatgctg gtgatggtaa atggctggcc ggttatcatc
     3961 gacaggctca gcgccgagcg caggatctgc ccgccacctt cgccctgtgc gccatccagc
     4021 gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag ggtatagata
     4081 acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata cgccatcggg
     4141 atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtggc acgaatttga
     4201 tcttccacgc tgaacagttt tttcgcttta gtacggctca ttacccgccc ggcaccgtgg
     4261 ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct ttttgctccc
     4321 atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcgcc tttacgcgtc
     4381 acgtagatct cttcaccaaa gtgctgttct ttttgcacat agttgtggtg acagttgatc
     4441 tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct ctgcaacgcc
     4501 gttaccacgt tttccatcat cgcatcgcgg ttaaggctgg cgaaaagctg cgcccaggcc
     4561 acggctctta ggtaatcatc aaagtattcc gtaccttcca taaagtacgc caggtcgcgc
     4621 gatggcaacg tttcaagctg gtcctgcatc tctttttgtg ccaaatcgat aaagtaagtc
     4681 ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca taatccacac ctggtccgac
     4741 tcatcaaggc agatttcaat aaagtggtta cccgttccca gcgttcccag gtgtttatag
     4801 ttgttagtgt taaggaaacg cggatatttt tgcgttaacc actgataacc ggcttcaagc
     4861 tcagcccatt tcgcatcgac gttaacaggc gggttttccc atgcgccttt atcacgttta
     4921 caacggccag tggtacgtcc gtgcggcacc gcagtttcaa tcgcctgacg cagctctgcc
     4981 aggttttcag gcaggtctgc cgccgttaac gcggtacgca gcgcgttcat tccacagcca
     5041 atatccacgc ccaccgccgc cggaataatc gcccctttgg tcgggatcac gctaccaatg
     5101 gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa aataaacggc
     5161 atcttcgccg tattaataag ttgctgacgc gcatcggctt ctaccggcac gcctttggtc
     5221 cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt gtttttctct
     5281 tttcgttgtt tgctgtcgtg ataattgcaa ccgtcgtgcc agaaaattaa ataagcagat
     5341 taatttttta attcattgtt tttaaatagg ttaaatcttt acgtccgtaa acggagattt
     5401 tccacaaaac caattcaccg ataatgaaat atcgtctttt ataaggatat ctaagatgcg
     5461 taaaacagtg gcttttggct ttgtcggtac cgtgctggat tatgccggac gcggcagtca
     5521 gcgctggtcg aaatggcgtc cgacgctttg tttatgccag caagaatcgt tagtcatcga
     5581 tcggctggaa ttgttgcacg acacccgctc gcgctcgcta ttcgaaacgc ttaaacgcga
     5641 tatcgccagc gtttcgccag aaacacaagt cgtgggcgtt gagattgaac tgcataaccc
     5701 gtgggatttc gaagaggttt acgcctgcct gcatgatttc gcccgtggtt acgagtttca
     5761 gccagaaaaa gaagactatt taattcacat caccaccggc acccacgtcg cgcagatttg
     5821 ctggtttctg ctggcggaag cgcgttacct gcccgcccgg ctgatacaat cttcacctcc
     5881 gcgcaaaaaa gaacagccgc gcggcgcagg tgaagtgacg attatcgatc tcgatttaag
     5941 ccgttataac gccatcgcca gccgctttgc cgaggaacgc cagcaaacgc ttgattttct
     6001 taagtccggc attgccacgc gtaaccccca cttcaaccgc atgattgagc agatcgaaaa
     6061 agtggcgatc aaatcccgcg cgccgattct gcttaacggt ccaaccggcg cgggcaagtc
     6121 atttctggcg cgacgcatct tcgagttaaa acaggcgcgg catcagttta gcggcgcgtt
     6181 tgtggaagtg aactgcgcca ccctgcgcgg cgataccgcc atgtcgacgc tgtttggtca
     6241 tgtaaaaggc gcgtttaccg gggcgcggga atctcgtgaa ggtttattac gcagcgccaa
     6301 cggcggaatg ttgtttcttg atgagattgg cgaactgggc gcagacgaac aggcaatgct
     6361 gctaaaagcc attgaagaga aaacctttta cccgtttggc agcgatcgcc aggtgagcag
     6421 tgattttcag cttatcgccg gaacggtgcg cgatttgcgc cagctggttg ccgaaggcaa
     6481 atttcgcgaa gatctgtaca cgcggatcaa tctctggacc ttcaccctgc cgggtctacg
     6541 ccagcgccag gaagatattg aaccgaacct ggattatgaa gtggagcgcc acgcctcact
     6601 cactggcgac agcgtgcgtt ttaacaccga agcgcggcgc gcctggttgg cttttgcgac
     6661 ctctccccag gcaacatggc gcggtaactt tcgcgaactt tctgccagcg tcacgcggat
     6721 ggccaccttt gccactagcg gacgcatcac tctggacgtg gttgaagatg agataaaccg
     6781 tctgcgctat aactggcagg agagccgccc ctccgcgctt acggcgttgc tgggcgcaga
     6841 ggcagaaaac atcgatctct tcgaccgtat gcaactggaa cacgttatcg ctatctgccg
     6901 ccaggcaaag tcgctttccg cagccggacg ccagcttttt gacgtttcgc gccagggcaa
     6961 agccagcgtc aatgacgcgg atcggctacg caaatacctg gcgcgttttg gtctgacgtg
     7021 ggaagccgtg caggatcagc acagctccag ttgaatatgg tggtccgtca gcacctgcat
     7081 cacgcttact ggcggcgggg cgtcggtgta gacggcatct accatgctga tgctgcccat
     7141 attgaccatc gcgttacggc caaatttcga gtgatcgaca accagcataa cgtggcgcga
     7201 gttctcaata atggcgcgtt tggtgcgaac ttcgtggtaa tcgaactcca gcagcgagcc
     7261 gtcgctatcg atgccgctta tccccagaat gccgaaatca aggcggaact gggagataaa
     7321 atcgagcgtc gcttcgccaa tgatcccgcc atcgcggctg cgtaattcgc caccggcgag
     7381 aataatgcgg aaatcttctt ttaccatcaa cgtgttagca acattgagat tgttggtgac
     7441 aatacgcaaa ttgctgtgat taagcagcgc gtgcgccacc gcttccggcg tggtgccgat
     7501 atcgataaac agtgtcgagc cattggggat ttgctccgcc actttgcggg caatcctctc
     7561 tttttcttcg gtctgggtgg ctttacgatc gtgccacggc gtgttaaccg aactggaagg
     7621 cagcgccgca ccgccatggt gacgcaggat caggttttgc tccgccagct cattgaggtc
     7681 gcggcgaata gtctgcgggc tgacggagaa atgctctacc agctcttcag tactgacata
     7741 accctgctgt ttaaccagtt cgataatacc gttgtgacgt tgtgtttgtt tcatttataa
     7801 atccctggaa ttattttcgt tttcgcgcat tgagcgaatc aacaaaagcc atcgctaaac
     7861 ccacggctaa cccggcgatg tgtgctccgt tcgccatcga catcccaaac aaatcaaacc
     7921 atccggcgac aatccagatc agcgcaaaga taattaaccc acgttgcagg taaatgccac
     7981 tttgcggatc gcgttcgcca cgtagccaga cgtagcccat cagcgcatac accacgccag
     8041 aaagcccgcc aaaccacggc ccgctgaatt tttgctgcac atagccgctt aacagggcgc
     8101 taatgagagt aataacaatt agcttaccgc taccgaggcg tttttccacc gcaccgccga
     8161 gataccacca ccagagcagg ttaaagagga tatgcatcag cgagaagtgc attaacgcgt
     8221 gggtgaagta acgccagaac tcaaatttca gtgttggatc gaatggccag gccagccata
     8281 acatcacttc ctgatcgccg agaatttgca tggcgataaa caccaccacg caggcgatca
     8341 tcaccaccca ggttaccgga cctgcgcgtt cacgcaaggc agcaaagaaa ggataacggc
     8401 gataatgcag accactgccg gtatggcccg cctgccagct ggccgccaga taacgcggat
     8461 ctgccgggtt ttcgagaaaa cgcgccagct ccgcccgcac gcgctcggcc tgggactcat
     8521 ccgccagcca gacatcgctt tggttatgtt gttgaatcgt gaggataaca ccctgcgtcg
     8581 ccatataatc aacaaacgcc tgcgccacgc gggggttagc aaaagaggta atcatcaaca
     8641 tcgttgctgt cgcttattcc acacaaaagg ggacagtata aagcgttacg cgccgtacgc
     8701 cacctctgcg ggaaactgac gctgccaggc ttcaaaaccg ccgtcaatgc tatagaccac
     8761 atcgtagccc tgttgcagca gatactgcgc cgcgcctttg ctgctattgc cgtgataaca
     8821 catcaccatc accggagtgt caaagtcgtt atcacgcata aacgcgccca gcgtgtcgtt
     8881 ggttaaatgg aaagcctgca ccgcatgccc catagcgaaa ctctgtggat cgcgaatatc
     8941 gaccagcacc gcctcttttt cctgcaactt ctggtgcgcg tcggcaacgt taatacattc
     9001 gaacagatcc atgcgtctct ctttctttac aaacaagtgg gcaaatttac cgcacagttt
     9061 acgtcgaagc ggcagataaa cgccataatg ttatacatat cactctaaaa tgttttttca
     9121 atgttaccta aagcgcgatt ctttgctaat atgttcgata acgaacattt atgagcttta
     9181 acgaaagtga atgagggcag catggaaacc aaagatctga ttgtgatagg tggcggcatc
     9241 aatggtgctg gtatcgcggc agacgccgct ggacgcggtt tatccgtgct gatgctggag
     9301 gcgcaggatc tcgcttgtgc gacctcttcc gccagttcaa aactcattca cggtggcctg
     9361 cgctaccttg agcactatga attccgcctg gtcagcgagg cgctggctga acgtgaagtg
     9421 ctgctgaaaa tggctccgca tatcgccttc ccgatgcgtt ttcgcctgcc acatcgtccg
     9481 catctgcgcc cggcgtggat gattcggatt ggtctgttta tgtacgatca tctgggtaaa
     9541 cgcaccagcc tgccgggatc aaccggtttg cgttttggcg caaactcagt gttaaaaccg
     9601 gaaattaagc gcggattcga atattctgac tgttgggtag acgacgcccg tctggtactc
     9661 gccaacgccc agatggtggt gcgtaaaggc ggcgaagtgc ttactcggac tcgcgccacc
     9721 tctgctcgcc gcgaaaacgg cctgtggatt gtggaagcgg aagatatcga taccggcaaa
     9781 aaatatagca ggcaagcgcg cggcctggtt aacgccaccg gcccgtgggt gaaacagttc
     9841 ttcgacgagg ggatgcatct gccttcgcct tatggcattc gcctgatcaa aggcagccat
     9901 attgtggtgc cgcgtgtgca tacccagaag caggcctaca ttctgcaaaa cgaagataaa
     9961 cgtattgtgt tcgtgatccc gtggatggac gaattttcca tcatcggcac caccgacgta
    10021 gagtataaag gcgatccgaa agcggtgaag attgaagaga gtgaaatcaa ctatctgctg
    10081 aaagtgtata acgcgcactt taaaaaacag cttagccgtg acgatatcgt ctggacctac
    10141 tccggtgtgc gtccgctgtg tgatgatgag tccgactcgc cgcaggctat tacccgtgat
    10201 tacacccttg atatccatga tgaaaacggc aaagcaccgc tgctgtcggt attcggcggt
    10261 aagctgacca cctaccgaaa actggcagaa catgcgctgg aaaaactgac gccgtattat
    10321 cagggtattg gcccggcgtg gacgaaagag agcgtgctgc cgggtggtgc cattgaaggc
    10381 gaccgcgacg attacgcagc tcgtctgcgc cgtcgctatc cattcctgac tgaatcgctg
    10441 gcgcgtcatt acgcacgcac ttacggcagc aacagcgagc tgctgctcgg caatgcgggg
    10501 gcgataagcg acctcgggga ggatttcggt catgagttct acgaagcgga actgaaatac
    10561 ctggtggatc acgaatgggt ccgccgcacc gacgatgccc tttggcgtcg cacaaaacaa
    10621 ggcatgtggc tgaatgcgga tcaacaatct cgtgtgagtc agtggctggt ggaatatacg
    10681 cagcagaagt tgtcgctggc gtcgtaagga tgatgcccgg ttgaagtgca accgggctat
    10741 ttggattcct tagactattc attgtaaata attgaataac ggcccctgag ctaaaatcgc
    10801 caaacttaag cacgttaccc aatcgcccca cttcttcctc tggtttctat tttttctgca
    10861 ccaatggcaa taatatcccc atgcaaaacc aagctgtgca ctaatcgggc cgacaaaatc
    10921 agtccaaggc tgcggccaaa aaggggaaaa attcagggga ggccagacaa acgtcagaaa
    10981 gaaatttcgg atattttcca gcaacgctga tttctctgga gaaatataaa gaatgaaatt
    11041 cgagagaatt aaccagaaat agaccggcga aaagaccagc cagcaatgca gtctgttttt
    11101 taattcagga ggcgttctgc gggagaattg ccaaccgatg ccagccatta cactgaaaaa
    11161 aagtgcatat tcaaccatta actcaagttg taattcctga agagaaatac gcacaaccag
    11221 gagcggaaag gagaatatta tttctgccag aaaagttaac gtgaatataa gtacggctaa
    11281 gacaataacc gtcggccata ccaataatgg cacgataaca aaaccgagaa ttacaggggg
    11341 aatataaagc cggaatttaa tcatttttat tttcctggtg attcggatga tgcgtcatac
    11401 atcgcgcata aacactcatc aattatccag tcatcttccc agacctgata ttctgtcatt
    11461 ggttcgggct gttttagcgg gttctcaatc gcttgccgcg ctctttcttt cagtaaagga
    11521 tgattatttc tagcccaggc attgatttgg gtggtaatgg cgttatcgat atcgtcctgg
    11581 ttatctctga tataatcctc acaatatctg ttcaataaac aggcaagaga aacggtcgac
    11641 caaaagccgg gaactgaatt aatggcatcc agcaatggcg caagagcgat atcggcacgg
    11701 aaaaacattt ccagcatata ctgtaattcg acagcttcat cgccggatac ccaatcagtc
    11761 atatactggc aggcaaatcg ctgtaaaatt gcccgttcat ccgcacgcca gttttctttg
    11821 ttggcaaggt tgatacggcg cagagaaaaa actacgtgaa tattgtcaaa atgcccctgt
    11881 gcaacaaatt ccagtaaacg cggcagaaaa tagcggacct catcgctatt ttgcggatca
    11941 ggccctgggc tactattcca ggcattgatt aaggaatacg ggatcgctcg taacgatgta
    12001 ctccgtaatg ccttttgttc ctgctgactc aggcaatatt cacaacaaac cacaaatttc
    12061 tgcggcaccc gataacgacg aaaggtgtga taaatctgct caattaatgc ctgcatggcg
    12121 gggcagggtt gcatagttac cccctcgccc gctgatcgcg tgtaaacggt tgatagacaa
    12181 aacgagcatt cgccgcgttc aggcgaataa gggcgctacg cgaaagggaa aagctggggc
    12241 gaatgcaact attgcagtct cgacagcttt cctctgcgca attttccact gagtaatgac
    12301 gcgtgtagag tttcagttcg gcctgcttat cgcgaaatcc gcgtaaactg ttattgttat
    12361 gaaatttcca ggcatcttta aagacctgaa agatatagac atcctcaatc caggagtcac
    12421 attttttaag cgggcgcttt gctattatca cccgctcatc cggatttagc atcgccgtaa
    12481 attgtacggt gacgtcattt gtcgtgttca ggtcacattg aacatctggt acatcactga
    12541 tataggggtc aaatccaaaa tcagcaccac aggcacaaag acggctaatg gtggcggtat
    12601 cgagggaaaa tcccggccat accttgctgg aggttaagcc aatatttaat gtgacatcat
    12661 atcgaagcaa tccctcaaga ccgtcagcac cgacgaactt ttccccttca tccagggcct
    12721 ggttaataag ttgctgaaca atgccttcat cccagtatgc cgatttgtag ttggcaatcg
    12781 taatatccac gcctgtgaag ggatcagtac cggcaaggct tgcaaataat gtattaacta
    12841 attccatttt tattatccag ctttaaggta gcgtcttata catcgggaca taacctacat
    12901 cttccaccaa actctgtccc tgcggcgtta aaaaccactc caacaatttt tgtgtctctg
    12961 acgtcgtatt ttcccgcgtt atcataaagg catcgacgat atagggatat ttgccgttgc
    13021 gaatgttttc ggcagtcggt gcaataccgt taatcgctag cagttttata tttttatcgg
    13081 cgttcatttg tgttgcgtaa taacggaagg tgtaacctat tgcgttgttt gtattacggt
    13141 attcggcaac gactttaatc attccctcca tcatacttgc cacttctgtt tcctgcggcg
    13201 agatcatgcg gacatttttc atgacctggg attgcatcac cgtctggcta ccagagtctt
    13261 ccgggcgttg ccaggtctgg atctcctgat cgttaccgcc aacagtacgc cagttggtaa
    13321 ttgcaccact gaagatgtca cgcacctgtt gttcagtcag ggaattaacc ggattatccg
    13381 cattgacgat gaaaacaaac gcttcacggg caaaaggcgt gtacagcaaa gtaacgcccg
    13441 attcctcagc gcgttttttc tgcccaccgg aaggttgcgc cacaaaaata atatcagcat
    13501 cacctttaac aatcctgtta tacgcatcgg gggtacgaga gctctccaga tattccctgg
    13561 tgtgaaaatc ttccggtata acgcttaaag cataaaatgc agaagcataa attgggtacg
    13621 ccgccgtagc accatcgagt cgcggccagt tttgcgtaaa ctgaatttgc ggtttaccac
    13681 gcagtggtgt cagttgatta ttgagtttat cgggtcgcca ggcccaggta tcaatatttt
    13741 cgcgcacacc gacgccggga ttgagctgtt tatataaata accctgataa ccagcgacaa
    13801 aaccaagcat gagaaggata aacaggaggg aattacggca acgtagcccg gatgtaccgg
    13861 ttaaaggatg tctggttggc cggccgtacc ccagcgaaaa actgatatgc gcgccaacgg
    13921 gtataatgac tatccaccag tactcacctc cgactactga gactaacaaa acgcccagcc
    13981 aggggagcat aagatcagcc aaccaacccg atcccatctg gtgaaattga taatctgcca
    14041 gatgcagcgc gattatccag ccaaccagga ttaataaacc gggtactaaa aaaagacatt
    14101 gtcgccagac ggctatttct cttttttctg gtgcactatc gcctgcccat actcgccccg
    14161 agaaaaatag cattaatgcg cccagcgcca gcataaaaat gttgagcaga aggtaacttg
    14221 ttggcgtgat aaggatatta caaacttcaa taataccggc aagtccgaca cccagcatag
    14281 caacaaccgc cgccaaaaat tgcgtcagta tggggatgcc gaaaaaagtc attaccagcg
    14341 aggacaaaat ccattttctg ttttgcatta ttctttccat tctttttgaa tggtgaaatt
    14401 atactccccg agtccccttg ccccttctgg acacttttcc gaaatgatgg cggaaaaaaa
    14461 cgggagcctt tggccccgtt ctatttattg gtgaacttac aatctcaccg gatcgatatg
    14521 ccagatatga tcggcgtact ctttgatagt acggtcagaa gagaagtagc ccatattggc
    14581 aatgttcagc atcgcttttg cggtccactc ttcctgaagc tcgtagagtt cgtcgacttt
    14641 atcctgacaa tcgacatagc tgcgataatc cgccagaacc tgatagtgat cgccgaagtt
    14701 gatcagcgaa tcaaccagat cgcgatagcg acccggatct tccggactga atacaccgct
    14761 gccgatttgc gtcagcacct gatgcagctc ctcatctttc tcgtagtatt cacgcggttt
    14821 gtagccctga cgacgcagtt cttccacttc ttccgcagtg ttaccaaaga taaagatatt
    14881 gtcagcaccg acatgatcca gcatctcgac gttcgcaccg tccagcgtac cgatagtcag
    14941 cgcgccgtta agcgcaaact tcatgttact ggtgccggaa gcttccgtcc ctgccagcga
    15001 aatctgttca gacagatctg ccgccggaat gatcaactgc gccaggctaa cgctgtagtt
    15061 cgggatgaac acgactttca gcttatcgcc aatctgcgga tcgttgttga tcactttcgc
    15121 tacgtcattg atcaaatgaa taatgtgctt cgccatgtaa taggccgaag ccgccttacc
    15181 gccaaaaata ttcacgcgcg gtacccactt cgcatccggg tcggccttga tgcggttata
    15241 gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa tacgtttgat
    15301 ttgtacatcg aacaacgcct ttggattcac caccacattc agctgctggg cgatatactc
    15361 tgccagacgc tttttgttct ccagcttcgc ctgatgcaca gcgtgattaa ccattgggaa
    15421 atcacagtgt tgttgcagct cattaagcag gctaaggtcg gtgcgccagt tacggcccag
    15481 gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc gctagccagc gacgcggcgt
    15541 cacaccgttg gtgacgttgg tgaaacgacc cgggaagatt ttcgcaaagt cggcaaacaa
    15601 cgattgcacc atcagattag agtgcagttc cgatacaccg ttaactttgt ggctcacaac
    15661 aaccgccagc caggccatac gcacacgacg accgttggat tcatcaatga tcgacgcccg
    15721 tcccagcaga tcggtatcgt tcggatactg ttcctgcaag gttttcagga aatagtcgtt
    15781 gatttcaaag atgatctgca ggtgacgcgg cagaatttta cccagcatat caaccggcca
    15841 ggtttccagc gcctcgctca tcagcgtatg gttagtgtag gagaagacct gacaacacac
    15901 ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc agcagacgca tcatctcagg
    15961 aatcgacagt accggatggg tatcattgag atgaatcgcg attttatccg ccaggttatc
    16021 gtaggtttta tgcaactgat aatggcggct taaaatgtcc tgaatggtcg aggaaaccag
    16081 gaagtattcc tgacgcaggc gcagctcacg cccggagtag gtggagtcat ccggatacag
    16141 tacgcgagat acgttctcgg agtggttttt atcttccact gccgcgaagt agtcaccctg
    16201 gttgaattta ccgaggttaa tttcgctact ggcttgcgca ctccacaaac gcaacgtgtt
    16261 ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa gcgactccca gaatctcttc
    16321 ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa aacggacttt
    16381 atagcgcgtg ttgtggcgtt tgaattccca cgggttaccg tattccagcc agtagtctgg
    16441 cgactctttc tggctaccgt taacgatgtt ctgcttgaac ataccgtagt catagcggat
    16501 gccgtaaccg cgccccggca accctaacgt cgccagagaa tcaaggaagc aagccgccag
    16561 acgtcccagg ccaccgttac cgaggcctgg gtcattttct tcatcaatca gctcttcgag
    16621 atttaacccc atcgcttcca gtgcgccctg tacatcttcg taaattccta gcgacaacat
    16681 ggcgttggag agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa cctgacgagt
    16741 ttcttgcgac aactgggcac ggtttgaacg taaccagcgc tccacgagac gatcgcgcac
    16801 agcaaataac gttgcgttca gccattcatg tttattggcg acgaccgggt cctttccaat
    16861 cgtaaacatc agcttgtaag cgatagagtg cttaagagct tctacgctaa gcgtgggcga
    16921 tgaatatgta aacggagcat tcatataggc gtttcctgaa aactatttca agcgatagta
    16981 aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa tccattgcca tagcctgacg
    17041 ttgcacaaac cgccacagtg aaggacggga ccacagtaca aaagcacgtc gaatagcccg
    17101 taacagcgac caggcattac tatcttcaaa gacaaaccca ctggcgacgc catctgcaag
    17161 gttctcaaga gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc gcactaacgg
    17221 cagcgtaccg tacttcaatc cataaagttg cgttaagccg cacggttcaa aacggctggg
    17281 caccagaatg acgtccgcgc cgcccataat gcgatgcgaa aatgcttcgt gatagccaat
    17341 ctgaacgccc acctggccgg ggtattccgc tgccgccgca aggaaacctt cctgcagcac
    17401 cggatcgccc gcgccgagta gcgccagctg cccgccctgc tccagaaggc ccggtaaggc
    17461 ttccagcacc aggtcgagac ctttctggct ggtcagacgg ctcaccactg caaaaagcgg
    17521 cactttatca tcaaccttaa gccccattgc gatttgtaac tggcgcttat tttccgcttt
    17581 atcttccaac gtatcgcggg tgtagcgcga ggccaacagt aagtccgtct ctggactcca
    17641 gattttctcg tccacgccgt tcagtacgcc ggaaagacgc ccttcacggt gacgctgttg
    17701 caacagacct tccataccgt aggcaaactg cggttcggtg atctcgcgag cgtaggttgg
    17761 actaaccgcc gtaatgtgat cggcatagta cagaccggcc ttcaggaaag agatttgtcc
    17821 gttgaattcc agcccatgaa tattaaagaa tgaccatggc aattggatgt cattcatatg
    17881 atgtgcataa aacatgcctt gataggccag gttgtgcaca gtaaacaccg acttcgccgg
    17941 acgcccgcgc gccgccagat acgcaggcgc aaggcctgca tgccagtcgt gcgcatgcac
    18001 cacatcagga cgccagaatg ggtcaagccc gctggccatt tctgccccaa cccaccccag
    18061 cagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt gatacgggct
    18121 tcccggacga tcatagagat gcggcgcgtc aatcaggtat atgccaaccc cgttgtaatg
    18181 accgaacaac agcgtgatat gtccggcgaa ggtatcacga cgggatacta cctgcgcatc
    18241 ggtaacgcca cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt caacgccgtc
    18301 tgcgatttgt gctgcgggta atgccccaat aacatcagcc agaccgccgg ttttaagcag
    18361 cgggaacatc tctgaacata catgtaaaac ctgcattatc gctcctgttt atgccctaac
    18421 ttccgtagca tttcgcgcgt taccagcacg atgccttctt ctgaacgata gaaacgacgt
    18481 gcatcttcct ctgcgttttc accaatcacc atgccttccg gaataacaca agcacgatcg
    18541 atgacgcagc ggcgcagacg gcacgagcga cctacccata cttccggtaa caatacggcg
    18601 gaatcaatgt tgcagaatga attcacgcga acgcgcgaga acagaacgga ctgcaccacc
    18661 accgaaccgg agatcacaca gccgccggat accagtgagt taagggtcat cccgtggcta
    18721 ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt gcgaattggc
    18781 caattgcgat cgtacatatc cagttccggc accacagagg ccagatcgag gttcgctttc
    18841 cagtaagctt ccagcgtacc cacatcgcgc cagtacggct cggcatccgg gtcggattgt
    18901 acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg cttcggtgat cttgggaatc
    18961 aaatctttgc caaagtcgtg gctggagttc tcatcacgat cgtcttcttc cagcagttca
    19021 tacagatagt cggcgtcaaa gacgtagata cccatactcg ccagagattt gctcggatcg
    19081 ttcggcattg acggtgggtt agcaggtttt tcaacgaatt cgataatttt atcgttctca
    19141 tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg gtactggcat acaagcaacg
    19201 gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac gcgagtagtc ttgcttgtag
    19261 atatggtcgc ccgccaggat caccacgtat tccgctttat aacggcggat aatgtcgagg
    19321 ttttgggtga ccgcatctgc ggtgccgcga taccagtttt cccctttcat tctctgctgt
    19381 gctggcagca gatcgacaaa ctcgttcatt tcttcattga agaatgacca gccgcgctga
    19441 atgtgctgca ccagagtgtg ggactggtac tgggtgatca cgcccatacg acggatcccg
    19501 gagttgatgc agttagacag cgcaaagtcg ataatgcgga acttaccgcc gaagtgtacg
    19561 gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc tcccgccagt
    19621 atcagggcaa cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc gttcttctct
    19681 aaactaacca tgactaactc cttttttatc atctctggaa cacacacaat ccgtgtgcag
    19741 gtccctgcca gacagccgta atcactgggt tatcctctcc agcgaatggg ggaatggcgt
    19801 gccactcccc agcaggtaaa acaatctctg ttacctcaag cgtggcgtta attgcgatca
    19861 aaaagcgatc cgagagcaga atttgcagct gtttcggccc gttttgccac tcatccgtgc
    19921 ttaaaggttg agcatatcga tttagccaac ggacattgcc gtcgccttct tcccaccagc
    19981 gattctccac caaagcggga atgcgcttgc gcagatggat taacgcggcg gtaaatgcgg
    20041 ttaaaccact gcttgcctgc gaccagtcca accaggttaa ttggttatcc tgacagtagg
    20101 cattgttatt gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac atcggcgtac
    20161 cctgggagag caacaacgtt gttaacaggg cgtgaatgct gtcgcgccgc cgttcaacca
    20221 ggtcaagaga accgcctaac ccttctttac catgattgtt actgtaattg ttgttggtcc
    20281 cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg gttgaagcaa acgcagtcgc
    20341 gaagcgtaaa accgtcatgc gcggtgacga gattaatcgc ggcactcggc agacgaccat
    20401 tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc aaacgccccc agaggcaaat
    20461 cataatgtag ccagaaacga cgggcagcat cgcggaaatg atcgttccac tcggcaaaca
    20521 gcggcgggaa atttcccacc tgataaccac caggagcgat atcccacggt tcagcaatta
    20581 acttcacctg cgagagcacc gggcagttct ggatagcggt aaacaacggc gcatcctgac
    20641 ggaactctgg cgtacggccc atgactgccg ccagatcaaa gcggaaacca tcgacgtggc
    20701 aggtttctac ccaataacgc aggcaggcgc tggcataatc caccaccgcc ggatgactca
    20761 aattgagcgt gttgccgcaa ccggtccagt tgtgataatc gccgtcttct cttatccaat
    20821 aatagctacg gttatcgatc ccacgcagcg agaataacgg gccgtcgagg tccagttccg
    20881 cactatggtt gagcacgata tcaagaatga cttcgatacc cgctttatgc agtgctttga
    20941 ttgcatcgcg aaactcatcc agcgccgttt ctggcgagca ggcatacgcc ggatgcagcg
    21001 caaacatcgc caccgggttg taaccccagt agttacttag ccccatgcgt tgcagacgtg
    21061 gttcactggc aaactgcgcc actggcagca gttccagcgc ggtaatgccc aattgtttca
    21121 aatagttgat catcaccgga tgcccgaggg ctttataagt gccacggatc tcgaccggga
    21181 tctccgggtg caagtacgtt aatcctttga catgggcttc ataaatgatg gtgctgcccc
    21241 acggcgtgcg cggcggggca tcatcttccc agtcatagtg atcaaccacc actacgcatt
    21301 tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt atgaccggcg tgcagcagcg
    21361 ggttatcttt aaactccccg tcaatttgcc gcgcgcaagg atcaatcaac aacttcgccg
    21421 ggttaaagcg atgcccctcg gcgggttgcc aggggccatg aacgcgataa ccataacgca
    21481 aacccgggcg cgcatccggc agataaccgt gccaaatgtc gccactgtgc cctggcaagt
    21541 catagcgatg ttcctggcca ttggcgtcaa agacacacag ttctacccgc tcggcatgag
    21601 cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg ggagcgggtt
    21661 tgccaatggc gagttgtgtc attctgcctc ccgaaccagc cagatagtgg ccagcggtgg
    21721 tagcgtcagg cttagtgaat gctgacgacc gtggctggca atctcatcgc tgtgtaccgt
    21781 gccgccattg cctgcattac tgccgtgata gtgcatggaa tcggtattga ggatttcacg
    21841 ccatttgccc ggctggttta tgccgaagcg ataatcatga cgcggtaccg gcgtaaagtt
    21901 actggcaacg atgatttcgt taccctcttt atcgcgacgc acaaagatca gcaccgagcg
    21961 ttctttgtca tccaccacca gccattcaaa gccgtacggg tcaaaatcca gttcatgcat
    22021 tgctttatgg tggcggtagg tgaggttcag atcgcgcacc agacgctgga caccgtggtg
    22081 ccagttatcg ccgccttcca acagatgcca gtcgaggctg gcgtcatggt tccactcgcg
    22141 gccctgggca aattcgttac ccatgaacag tagtttcttg cccgggaatg cccacatcca
    22201 gccatagtag gcgcgcaggt tcgcgaattt ctgccatgcg tcgcccggca tgcggtcgag
    22261 aatcgatttt ttaccgtgga ccacttcatc atgcgacaac ggcaggacga agttttcagt
    22321 gtagttgtag agaatcccga aggtcagttt atcgtgatga tactgacgat aaaccgggtc
    22381 gagcttcatg tagtccaggg tgtcatgcat ccagccgagg ttccacttgt accagaagcc
    22441 cagaccgccc atatcctgcg gacgagaaac gccagggaaa tcggtagact cctcagccat
    22501 tgtcaccgca ccggaaacct gctcaccaag aatacggttg gtattacgca agaattcaat
    22561 cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc cactccccct ctttacggct
    22621 gtagtcgcga taaatcattg acgccaccgc atcgacgcgc agcgcatcaa taccaaaacg
    22681 ttcaatccag taaagcgcgt taccgacgag gaagttactg acttcacggc gaccatagtt
    22741 gtagatcagc gtgttccagt cctgatgata gccttcacgc ggatcgctgt gttcatacaa
    22801 gttcgtgcca tcaaattcgg caagcgcaaa gtcatcagtc gggaagtggc ctggcaccca
    22861 gtcgagaatc acgttcagac cagctgcgtg tgcggcatca atgaaataac ggaagtcgtc
    22921 gcgagtacca aaacggcggg ttggtgcata caggccggtt ggctgataac cccaactgcc
    22981 atcgaaggga tgctcgttaa tgggcagtag ttcgaggtgg gtaaagccca tccatttagc
    23041 ataaggcacc agttgatcgg ccagctcgcg gtagctcaac cagaaattgt tgtcggtgtg
    23101 gcgacgccag gaacccaggt gaacttcata aatagagatt ggcgcatcaa actgattcgc
    23161 ttttttgcgc tcttcagtct gtacaacctt ttccggcagc ccgcaaataa gagacgcggt
    23221 ttccgggcgc atttgcgctt cgaaggcata agggtcggac ttcagacgca agttgccatt
    23281 ggcatcaatc atctcgtatt tatagagctg accgttatgc gccccaggga taaacagttc
    23341 ccagatgccg ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc agtagttgaa
    23401 ttgcccaacc accgagaccc gacgggcgtt tggagcccag acagagaaac gcgtacctgt
    23461 gacgccatcc atagtatctg catgcgcgcc taaggtttca tacgggcgca ggtgagtacc
    23521 ttcagataat agccaggcat ccatttcctg gattagcgga ccaaaacggt aaggatcatc
    23581 aatcaggttt tgctgaccat gccagacaac agccaactga tagcggaaaa aattcttacg
    23641 tcgcggaatg acgccgctaa agaatccccg tgagtcgaga cactccagtt ttgcgagttt
    23701 gcgcccggtt ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa gggcacggac
    23761 ttccagtccc gcggtggttt tatgcattcc cagtacggaa aaaggatccg caaaatggcc
    23821 tgcaattagc gcgttaatca cgtctctatc gatacgatcg gacatgcttg tcttcctgtt
    23881 ttattgtgtc accccatcca attggatttt tgacttctgg ttgtgacatt tttttgacct
    23941 gaacggcgca gcactctgtg catcctctct gcgtcgtcct cacttcaggt aaggctgtga
    24001 atactcatgt attcagccac ccttaaagaa tagccaatgc tctatttaac tcccggtaaa
    24061 tcatgaaaca tctgcgctta ctcctgtatt acgcactaac aggggcggca tcgcgcccca
    24121 gatttaatga ataaagatta cgccagttga cgaagcatcc gacgcagcgg ctccgcggcc
    24181 ccccacagca gctggtcgcc cacggtaaag gctgacagga actctggtcc catattcagc
    24241 ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg ggttagctca
    24301 cgcatagtga tttcccgatc gttcggaacg actttcgccc acggattgtg cgcagccagc
    24361 agttcttcca cggtcggaat agacacatct tttttcaatt taatagtgaa tgcctggctg
    24421 tggcagcgca atgccccgac acgcacacat aaaccatcta ccggaattac ggaagatgtg
    24481 ttgaggatct tgttggtttc cgcctgccct ttccactctt cgcggctctg accgttatcg
    24541 agctgtttgt cgatccacgg aatcaggcta cccgccagcg gcacgccaaa gttatccacc
    24601 ggcagctcac cgctacgggt taaggttgtg actttgcgtt cgatatcgag aatagcagag
    24661 gacggggtcg cgagttcatc tgccacatgg ccatacagat ggcccatctg ggttaataac
    24721 tcacgcatat gtcgcgcacc accgccggaa gcggcctggt aggttgcaac ggacacccaa
    24781 tcaacaagat cattggcgaa taaaccaccc aacgacatca acatcaggct tacggtacag
    24841 ttaccgccaa caaaagtcct gatgccatta tttaatccgt cggtaatgac gtcctgattg
    24901 acggggtcaa gaatgatgat ggcgtcatct ttcatgcgca gagacgatgc tgcgtcaatc
    24961 cagtaacctt gccatccgct ttcacgaagc tttggataga tttcgttggt ataatcgccg
    25021 ccctgacagg tcacaatgat atcgagggcc tttagcgcct ccagatcaaa ggcatcctga
    25081 agtgtgccag tggttccgcc aaaagacggc gcagcctggc caagctgaga agtagaaaag
    25141 aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat gagaacggag
    25201 ccgaccatac cgcgccagcc gataaaacca acatttttca taagcgtttt ttcctgcaaa
    25261 gatgtgtgct gtataaatgt gccggtctcc tcttggcaca tctttcacca tacaaaaagc
    25321 agccaaagtc gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat gcgacttatc
    25381 ctgctttgtt agcacgcaga aagtccgcgg caattatcag ggaatttgag ttatgaatga
    25441 aatcatttct gcagcagttt tattgatcct gattatggat ccgctcggaa acctacctat
    25501 tttcatgtcc gtactgaaac atactgaacc gaaaagacgg cgggcaatca tggtgcgaga
    25561 gttgcttatt gctctcctgg tgatgctggt gttcctgttt gcgggtgaga aaattctggc
    25621 atttctcagc ctgcgagcag aaaccgtctc catttctggc ggcatcattc tgtttctaat
    25681 cgccattaag atgattttcc ccagcgcttc aggaaatagc agcgggcttc cggcaggtga
    25741 agagccattt atcgtgccgt tggctattcc gttagtcgcc gggccgacta ttctcgccac
    25801 gctgatgttg ctgtctcacc agtacccaaa tcagatggga catctggtga ttgctctgct
    25861 gctggcctgg ggcggcacct ttgtcatcct gctacagtct tcgctatttt tacgtctgct
    25921 gggcgagaaa ggggtgaacg cacttgaacg cctgatggga ttgattctgg tgatgatggc
    25981 aacccagatg ttcctcgacg gcattcgaat gtggatgaag gggtaacgag gagagttttt
    26041 cgaaaagaac ccggtagcgt cacaactacc gggcgaacaa acttaactca acagctggaa
    26101 cgcaatcatc ccaacgatgg caccgacagt gccgaggatg gtttccatca tggtccaggt
    26161 tttcagcgtt tcggcttcgg tcgcgccggt aaatttaccg aacaaccaga aaccggcgtc
    26221 gttaacgtgg ctgacaacaa tcgaaccacc agcgatacaa atcgacagcg ccgccatttg
    26281 cgcaccggag tagttcagtt gttcaataac cggcatcacc agtcctaccg ccgttaaaca
    26341 ggctacggtg gcagaaccct gaatgatgcg cactgcagcg gccagcacga agcaggtgat
    26401 ggctatcggc aggcccatgc cagttaacgc ttcgcccagt gccggaccta cgccagagtc
    26461 aaccagcacc tgtttgaaca cgccacctgc accaatcacc agcagaatga tccccgccgg
    26521 ttgcagcgcg tgaccgcaaa tctccatcac tttgtctttt ggcatgccct gacgcattgc
    26581 caggccataa atcgctacca gacaagcaac cagaatcgcg gtaaacggat gaccaataaa
    26641 ctcgaaccat tcgtaagcgg tagagccttc tggcacaaaa cgcgcggcaa tggttttcag
    26701 ccccaccagc accagcggca acaggatcag cgacaggctg aatccgaaag atggcatttt
    26761 gccttcgccg agatgcggtt cgctgatgtc gtcaggaata tgcaactcca cgtagcggct
    26821 gatgaaatta ccccacagcg gcccggcaat aatcattccc ggaattgccg cacacaggcc
    26881 aatcaggatc atccagccaa aatcggcgtt catctgcgat gccagcagca ttggcgctgg
    26941 ccctggcacc aggaacgccg ccgctgccgc cacgcctgcg aataatggga ttaccagctt
    27001 caccaggttc gtaccggtgt ggcgcgccat tgagaaagca acgctaatca gcagaacaat
    27061 cgccacttca aagaacagcg gtagcgcaca gaccagcccc gcaaggccga tggcataatg
    27121 cgcgcggctg tgaccgaagg atttaagcat tttgacggca atctgatcga ctgcgccggt
    27181 ttcatgtaag atcttgccaa acatagctcc cagggcgaca accaccgcca ggaagccgag
    27241 ggtgcctccc atcccttttt ccatcgtcgc tgcgatttta tcgagcggca taccggaaaa
    27301 aaggccagcc cccatggaca ccaccatcaa agccaggaaa gcgtgcatac gcgccttcat
    27361 gactaaaaac agcagcagta aaacagaccc tactgctgtt aaaacaagcg ttaatgtagt
    27421 cactacttat ttgccttttt taataacctc aatggtgctt gccacaacac cttccagcgg
    27481 ttgatcgata tccaccacca gtacatcagt ttcgtccgca cccggctcct gcagcgtttc
    27541 aaactgcgtc accaacattt gggttttaaa gaaatggcct ttgcgcgctt tcaggcggct
    27601 ttcaatcacg tcaaaatcgc ctttcaggta gatgaaagag agattcggat taccttcacg
    27661 cagcaagtcg cgatagtgtt ttttcaatgc agaacagacg atcagtgaca ctttattagt
    27721 gcgctgcata gcaaacgcgg cgtcgttcag cgcctgcaac cacggtttgc gatcgtcgtc
    27781 attcagcggt tcgccagacg ccattttttc gatattgcgc cgtggatgga ggaaatcgcc
    27841 atcaagaaac gcggcatgaa gttgatgcgc cacttcactg gcgaccgcag atttgccgct
    27901 gcccgatacg cccatcaaga cgtaaatgtg gtgatcatgg ttagtcgtgc tcaaagtggt
    27961 gcccccacaa tacaagaatt aaaaatgtta cgggtaactg ttatcggtaa cattgtccag
    28021 ccggacaata tcagaagcaa tatccatccg tgccttaagt gtataagtgt gagctacttc
    28081 aaatttgtag gcttaaatag atccgcccgg tgacaaggtg aaacctaaat ctaacatttt
    28141 cggtgtcaca gattcgccac gaatacgcgc cagcaggcgt tcagcgccaa tactgcccat
    28201 ccgctcacgc ggcgtcagca cgctcgctaa ccgcggctcc atcacctggc caatgtcatg
    28261 accgtggaaa ccggcaatcg ccatatcgtc aggaaccttc aatcccagac gctgacattc
    28321 aaacgccgcg ccgaccgcca ggtcatcgtt ggtacagaac acgccatcca gctgcggata
    28381 ttcccgccgc gcctggcgaa tcagttcaat accggaagag taagaagaag attgctcaac
    28441 catcacgcta tacggcacca gacctgcatc cagcatcgcc tgttcgtatc ccttctgttt
    28501 gatgatagta cgttcgtcga gacgcgcgcc gagataggca atgtggcaat gcccgcgagc
    28561 aataatggcg gtggtcatct ggcgtgccgc ttcaaagtta tcaaaaccga cggcgatatc
    28621 gaggcacggc gactggctgt ccatgagttc caccaccggg atccccgcca cttcaatcat
    28681 ctttaaggtg cgcggcgtgt gggtacgttc ggtgaggatc agtccgtcga tattccatga
    28741 gagcatggat tcgagacgtt cttgctccat ttccggttta taaccgtagt gcgccagcat
    28801 ggtctgataa ccgtgcgcgt cggtgacgct ttcgattccg cgtaatactt ccgcgaaaac
    28861 ctggttggtg agagaaggta acaggacgcc aatcgcccgg ctggtggcgt tagagaggat
    28921 atcgggcgca cgattgggaa tatagcccag ttcatcaaga gcggcggcaa tcttgccgcg
    28981 tagagcgacg gaaacctgct ccgggttgcg taaaaaacgg ctgaccgtca ttttggtcac
    29041 gcctacacgg tcagccacat cctgaagtac gggtcttttc tttttcatcg tcctgaaggt
    29101 acaaaagaga tagattctct tagtttaaca cggacgcatc agaaccttcc cggggaaaac
    29161 agtgcttgtt tacaattatt agatatacac taaatcaatt gcatcgcatt gtgctaacga
    29221 cgcggcaaaa ccccggaagc gtgcatcagc acgcgaccgg agcgagcgag gaagcctgca
    29281 aaaaggcagg cttcaaaaat gccgtggagc tttaaaccgg cggcagatcg aacagtaaca
    29341 cttcgctgtc gctatccgca tggatggaga ttgcctgctc atcccagatt gccagaccat
    29401 cgctggtcga ggctttcacg ccgttaatgg tgacattgcc tttcaccacc tggatccaga
    29461 cgcggcgttc agcggcaatc tgatgcaccg actgctcatc tttcagcaac gcccagcggt
    29521 acagttccat atcctgatgc actttcaatg aaccatctcg cgcatccggc gagagcacca
    29581 gctgtttgcc ctgtacggca tcgaagcgac gctgttcata acgcggcgta ataccgtttt
    29641 cttcaggcat gatccagatc tgatacagat gcagacgctc ggtgctgctt gggttgtact
    29701 ctgagtgacg aatacccgta cccgcactca taatctggaa ctcacccgcc ggaacctgct
    29761 ctttattgcc catgctgtcc tgatgctcaa cagtaccttc cagcacgtag gtcaaaattt
    29821 ccatatcttt atgcgggtga gtgccgaagc cctgccctgc ttcaatcacg tcgtcgttaa
    29881 tcacgcgcag cgcggagaag cccataaagt tcggatcgta atagttggca aaagagaaag
    29941 tatgccagga gtccagccag ccatgatttg catgaccacg ttcatttgct ttgcgtaagt
    30001 agatcatttg tattcgcccc ctgaatgatt tcgatgggct tagtgtggac ccgatctgcc
    30061 tgggatgata gagggtgaaa attgacccct ctgttcaaaa aaattgaact attcgagggg
    30121 ccatcaaact taattcgcga gagtcactgt ggagggagag gcttgctcaa atccgcgttc
    30181 aaggatttcc agattggtaa gaacttcaga ttccttgacg taatttggcg caccgtgggt
    30241 gatggtttga tacaacgcat cataaacgcg cccgtaatcg cccatctccg gcttcatctc
    30301 ttctctgacc gtcacgccct cgtcattgac atactccagc acaccgaccg aattatccgc
    30361 tgcgaatccc ggttcgcccg gcataatatt agccttcagg ctggtttcct gctggtcgat
    30421 accgtattta ataaacgaac ctttcttacc gtgaacgata aatttcggat aatcgatttt
    30481 caccagatgg ctggttttga cgatggcttt caggtcgcca taaaacagtt gcgcttcaaa
    30541 ggtgtcgtca ggattggctt tattacgcag gctgcggatg tcataagcga cgtgatccgg
    30601 gcgaccgaac agagaaataa tctggtccat cgtatgcaca ccaaggccat aaaacgcgcc
    30661 atcctgcggc agcccaggtt tggtttctgc caccgggcgg taatagtcaa aatggctttc
    30721 cacttcaaca atctctccca acttgccact ttcaatcgct tttttcgctg tcaggaagca
    30781 ggagtcaaag cgacgattct gatacggcgt gacggtcagc cctttgcttt tcgccaacgc
    30841 aaacagctct ttcgcctgcg caagtgtcgg agtgaacggt ttttcgacca gcacattttt
    30901 cccggcttcc agcgcgcgct tcgcgtactc gaagtggctg tccgcgtggg tgcagacaac
    30961 aaccagctta acatcgggat cgtttagtac ttcgtcgaga tcgctggtga aatggatatg
    31021 ggaataaatg ggagcctgtt cttccggctt cgcatggcga cgaaaaatat gcgcgacatg
    31081 ccagctatcc ttgcggttaa gtacatacgg cagatggtaa cgggtggtgc ttttgccgaa
    31141 gccaataaag gcgcagttga tgaccatgat ttcgtccttt ttaaggtggt tatttacacc
    31201 ttagcgcaaa gcggacgtgg ttcctactgg agtgcgcata acgaacacaa gcactcccgt
    31261 ggataaattg agaacgaaag atcaaaaaaa aagccagcac ccggctggct aagtaatact
    31321 ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc gagggtcttc ctgatcgcga
    31381 gacaataata atcattctca ttcgcacttg tccaacactt tttgcaaaaa aatgcatttg
    31441 actcgcattt gaaagtcaat gatgttgaaa gggacattta ccccaaagag gacaaaggaa
    31501 tgagtgagat agtaatacgc cacgcagaaa cacgggatta cgaggccatc aggcagattc
    31561 acgcccagcc ggaggtgtat tgcaacacac tacaggtgcc tcatccttcc gatcatatgt
    31621 ggcaggagcg actcgccgat cgtcccggca tcaagcaact cgtcgcctgt attgatggag
    31681 acgtcgtggg ccatctcacc attgacgtgc aacagcgccc acgccgcagt catgttgccg
    31741 attttggtat ctgtgtcgac tctcgttgga agaaccgcgg cgtcgccagc gccctgatgc
    31801 gagagatgat tgaaatgtgc gacaactggt tgcgggtaga tcgcattgaa ctaaccgtgt
    31861 ttgtcgataa cgccccggca attaaggtct ataaaaaata cggctttgaa attgaaggga
    31921 ctggtaagaa gtacgcattg cgtaatggtg aatatgtcga tgcatattat atggcgcggg
    31981 tgaagtaaga tagtgccctt tttctgagat ggaaaaaggg tgtcattcaa aatcgacata
    32041 ccttccttta ag
//
LOCUS       DAZKGS010000041        31287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKGS010000041 DAZKGS010000000
VERSION     DAZKGS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..3245)
                     /gene="acrB"
                     /locus_tag="ACHOQY_001832"
     CDS             complement(96..3245)
                     /gene="acrB"
                     /locus_tag="ACHOQY_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9677950.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3268..4461)
                     /gene="acrA"
                     /locus_tag="ACHOQY_001833"
     CDS             complement(3268..4461)
                     /gene="acrA"
                     /locus_tag="ACHOQY_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9677951.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4603..5250
                     /gene="acrR"
                     /locus_tag="ACHOQY_001834"
     CDS             4603..5250
                     /gene="acrR"
                     /locus_tag="ACHOQY_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9677952.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5378..8740
                     /gene="mscK"
                     /locus_tag="ACHOQY_001835"
     CDS             5378..8740
                     /gene="mscK"
                     /locus_tag="ACHOQY_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9677953.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQCDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8952..9113)
                     /gene="rsmS"
                     /locus_tag="ACHOQY_001836"
     CDS             complement(8952..9113)
                     /gene="rsmS"
                     /locus_tag="ACHOQY_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9677954.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9127..9654)
                     /gene="priC"
                     /locus_tag="ACHOQY_001837"
     CDS             complement(9127..9654)
                     /gene="priC"
                     /locus_tag="ACHOQY_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9677955.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9724..10101
                     /gene="ybaN"
                     /locus_tag="ACHOQY_001838"
     CDS             9724..10101
                     /gene="ybaN"
                     /locus_tag="ACHOQY_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9677956.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10254..10805
                     /gene="apt"
                     /locus_tag="ACHOQY_001839"
     CDS             10254..10805
                     /gene="apt"
                     /locus_tag="ACHOQY_001839"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9677957.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10934..12865
                     /gene="dnaX"
                     /locus_tag="ACHOQY_001840"
     CDS             10934..12865
                     /gene="dnaX"
                     /locus_tag="ACHOQY_001840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9677958.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12198..12262
                     /gene="dnaX"
                     /locus_tag="ACHOQY_001840"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12918..13247
                     /gene="ybaB"
                     /locus_tag="ACHOQY_001841"
     CDS             12918..13247
                     /gene="ybaB"
                     /locus_tag="ACHOQY_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9677959.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13247..13852
                     /gene="recR"
                     /locus_tag="ACHOQY_001842"
     CDS             13247..13852
                     /gene="recR"
                     /locus_tag="ACHOQY_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9677960.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13962..15836
                     /gene="htpG"
                     /locus_tag="ACHOQY_001843"
     CDS             13962..15836
                     /gene="htpG"
                     /locus_tag="ACHOQY_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9677961.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16017..16661
                     /gene="adk"
                     /locus_tag="ACHOQY_001844"
     CDS             16017..16661
                     /gene="adk"
                     /locus_tag="ACHOQY_001844"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9677962.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16897..17859
                     /gene="hemH"
                     /locus_tag="ACHOQY_001845"
     CDS             16897..17859
                     /gene="hemH"
                     /locus_tag="ACHOQY_001845"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9677963.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17856..18815)
                     /gene="aes"
                     /locus_tag="ACHOQY_001846"
     CDS             complement(17856..18815)
                     /gene="aes"
                     /locus_tag="ACHOQY_001846"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9677964.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18967..20271
                     /gene="gsk"
                     /locus_tag="ACHOQY_001847"
     CDS             18967..20271
                     /gene="gsk"
                     /locus_tag="ACHOQY_001847"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9677965.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20404..22080)
                     /gene="ybaL"
                     /locus_tag="ACHOQY_001848"
     CDS             complement(20404..22080)
                     /gene="ybaL"
                     /locus_tag="ACHOQY_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9677966.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22318..23538)
                     /gene="fsr"
                     /locus_tag="ACHOQY_001849"
     CDS             complement(22318..23538)
                     /gene="fsr"
                     /locus_tag="ACHOQY_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9677967.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23756..25408
                     /gene="ushA"
                     /locus_tag="ACHOQY_001850"
     CDS             23756..25408
                     /gene="ushA"
                     /locus_tag="ACHOQY_001850"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9677968.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25445..25924)
                     /gene="ybaK"
                     /locus_tag="ACHOQY_001851"
     CDS             complement(25445..25924)
                     /gene="ybaK"
                     /locus_tag="ACHOQY_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9677969.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26128..26922)
                     /gene="ybaP"
                     /locus_tag="ACHOQY_001852"
     CDS             complement(26128..26922)
                     /gene="ybaP"
                     /locus_tag="ACHOQY_001852"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9677970.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27060..27401
                     /gene="ybaQ"
                     /locus_tag="ACHOQY_001853"
     CDS             27060..27401
                     /gene="ybaQ"
                     /locus_tag="ACHOQY_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9677971.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27515..30019)
                     /gene="copA"
                     /locus_tag="ACHOQY_001854"
     CDS             complement(27515..30019)
                     /gene="copA"
                     /locus_tag="ACHOQY_001854"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9677972.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30281..31213
                     /gene="glsA"
                     /locus_tag="ACHOQY_001855"
     CDS             30281..31213
                     /gene="glsA"
                     /locus_tag="ACHOQY_001855"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9677973.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31216..>31287
                     /locus_tag="ACHOQY_001856"
     CDS             31216..>31287
                     /locus_tag="ACHOQY_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9677974.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIG"
BASE COUNT         7655 a         8305 c         8197 g         7130 t
ORIGIN      
        1 atgagatcct gagttggtgg ttcaattact ccttaatgtt cgtaggttat gcataaaaaa
       61 ggccgcttgc gcggccttag tgattacacg ttgtatcaat gatgatcgac agtatggctg
      121 tgctcgatat cttcattctt gcggctaaag cggcggcgaa ccaccacaaa gaataccgga
      181 acgaagaaga ttgccagtac cgttgcggtc accatcccgc ccattacacc ggtacctact
      241 gcgttctgcg cgccggaacc agcaccagta ctgataacca gcggcataac gccgaggata
      301 aacgccagcg aggtcatcag gatcggacgt aaacgcatcc gcactgcatc aagcgtcgct
      361 tcaatcagac ctttaccttc tttatccatc aagtctttgg cgaattcgac gataagtatc
      421 gcgttcttcg ccgacaaccc aatggttgtg agcaggccta cctggaagta aacgtcattg
      481 gtcaggccac ggaaggtggc agccagcaac gcaccgataa cccccagcgg aacgaccagc
      541 ataacggaga acggaatcga ccagctctcg tacagcgccg ccagacacag gaatacgaca
      601 atcaacgaaa tcgcgtacag tgaaggtgcc tggttgccgg agaggcgttc ctgataggac
      661 atccccgtcc agtcatagcc aacaccggta ggcagtttgc tcgccagttg ttccatcagc
      721 tccattgctt caccggtact tttacccggt gccgcctggc ctaagatttc catggatggc
      781 aggccattgt aacgttccag acgcggcgaa ccgtactccc aacgagaaga ggagaatgcc
      841 gagaacggca ccatctgacc atcagcagca cgaacatacc agtcgccgat atcatccggc
      901 agcatacggt atttcgcttc tgacatgacg taaactttct tcacacgacc gcggtcgata
      961 aagtcgttca catagctacc gccccatgca gcgcccagag tggtgttaat gtcattgata
     1021 gaaacaccca gcgcctgcgc tttttcccgg tcgatatcaa tcttaaactg cggggtatct
     1081 tccagaccgt ttggacgtac gctggtcaac atatcagggt gcttcgctgc ttctgcaagc
     1141 aactggttac gcgcctgagt cagtttttcg tgaccaaggc cagcctggtc aatcagctca
     1201 aagtcaaagc cggttgcagt acccagttcc acgattgcgg gcaggttaaa ggcgaaaacc
     1261 atcgcatctt tgatttgcga gaaagcgcgt gttgcacgca tggtaatcgc ttcaactttg
     1321 ttttcttcgc ccggacgatc ggcccagtcc ttcaaggaaa cgaacgcaat accggtattc
     1381 tgaccacgtc ccgcaaagcc gaagccgtta acggcgaaca ccgactcaac gttgttcttt
     1441 tctttggtca gatagtaatg cgttacctca ttgagcactt tctgcgtacg ttcctgcgtt
     1501 gcacctgctg gcagctgaac catggtcata aatacgccct ggtcctcatc tggcaagaag
     1561 gagcttggca gacgcacgaa cagataggcc atgccgacca cgatgatcag atacagcacc
     1621 aggtaacgcc ccgtactgcg cagaataccg cctacgctgt cggtgtagtg gtgcgtgctc
     1681 ttctcgaaca tgcggttaaa ccagccgaag aagccttttt taccttcccc gtgatcgcct
     1741 ttggcaatcg gtttcagcat ggtggcacaa agagccggag tcaggatcaa cgctaccagt
     1801 accgacagcg ccattgctga aacaatggta atagagaact gacgatagat agcaccagta
     1861 gaaccgccaa agaaggccat cggtacgaat accgccgaca gtaccatcgc gataccgacc
     1921 agagcgccct gaatctgccc catcgactta cgggtagctt cttttggcgg caaaccttct
     1981 tccgccataa cacgctccac gttttctacc acaacgatag cgtcatccac caacaagccg
     2041 atggcgagca ccatcccgaa cattgttagc gtgtttatcg agaagccaaa ggcggcaagg
     2101 acggcaaagg tcccgagcaa taccaccggt acggcaatgg tcggaatcaa cgtcgcgcgg
     2161 aagttctgca ggaacagata cataaccagg aacacgagga tgatcgcttc gaccagcgtt
     2221 ttaaccactt cgtgaataga gattttcacg aacggcgtgg tgtcgtacgg gtaaacaatt
     2281 ttcagacccg acgggaagaa cggttccatc ttcgccagtt cagcacggat tgccgcagcg
     2341 gtatccagcg cgtttgcacc ggtcgccagc ttgatcccca gaccggaagc cggttggccg
     2401 ttaaactctg cgatgatgtc gtagttctca ccacccagct caatcttcgc cacatcacgc
     2461 agcagtacgc gggaaccatc ctgattcact ttcagcagga ttttgccgaa ctcttcagta
     2521 gaggtcagac gcgtctgagc aataatagag gcgttaagct gttggccttt caccggcggc
     2581 gtaccaccga gctgacccgc cgcaacctgg gcgttctgcg ctttgatggc ggtaatgaca
     2641 tcaaccggcg ttagctggaa tttgttcagc tcattcgggt tcatccagat acgcatcgcg
     2701 tactgtgaac cgaacaactg aacatcaccc acgcccgacg tacggctgat ggcatctttc
     2761 atattcgccg ctacgtagtc agagatatcc tcctgcgtca tggtgccatc ggtgttgata
     2821 acgccgacaa ccatcaggaa gctgctggat gatttctcaa cgctcacccc ttgctgctga
     2881 acttcttgcg gcagcaacgg catcgccagc tgcagtttgt tctgcacctg aacctgcgcg
     2941 atatccgcat cagtaccaga ctcaaaggtc agggtgatct gcacggtacc cgtggagtca
     3001 ctgttagagg acatgtacat caggttatcg ataccgttca tattctgttc gataacctgt
     3061 gtcaccgtgt cctgcactgt tttcgcatca gcgccggggt aggaggcgga gatcgttact
     3121 gccggcggtg caatcgtagg atattgcgcc accggcagtt tgaggatcgc cagcccccct
     3181 gccaacatga tgataatggc gatcacccac gcaaaaatcg ggcgatcgat aaagaaatta
     3241 ggcatgtctt aacggctcct gtttaagtta agacttggac tgttcaggct gagcaccgct
     3301 tgcggcttgc tggttattat cagcggtaac ttcttgtgct tttacctgga caccaggacg
     3361 cactttctgc agcccactta ttactacgcg atcgcctgct ttcagacctt ctgtcaccag
     3421 ccacttatcg ccaatagcct ggcttgcaac gatcggacgg gtttccactt tgtcatccgc
     3481 gccaactacc agtacggtgg catcaccacg cggcgtacgg gttacgccct gttgcgggac
     3541 taaaatagcg tttggattaa gcccttcttc cagacgtgcg cgcacgaaca tacccggcag
     3601 cagagtgtga tccgggttcg ggaagatagc gcgtagggtg atagacccag tggtctgatc
     3661 aacggtaacg tcagagaatt ccagcgtacc gtcctgcggg aacttaatgc cgtcactggt
     3721 gatcagcgac actttggctt tgccgttctc ttgtttcagc gtgccattcg ccagttcctg
     3781 tttcaggcgc aggaagtcgt tgctggactg ggtcacatca acgtagatcg gatcaagttg
     3841 ctgcacggtt gccagcgcag tcgcctgacc gttctgtacc aatgcgcctt ccgtcacgtt
     3901 cgacttacca atgcgaccgc taatcggaga ggtgactttg gtgtaagcca gattgatccg
     3961 cgcagtttca acggcagctt tcgccgcagt taccgcagca ttcgcctgtt gcgcatcagc
     4021 cagagcctga tcgtactctt gcttactgat gtactgagta ccgagcagtt tctgataacg
     4081 attcaccgtc aattgcgcga tattggctgc agcctgggct ttcgccagat cacctttcgc
     4141 actgtcgtat gtcgcctgat aggtcgcagg atcaatctga tagagagaga caccagcttc
     4201 gatgtcgcta ccttctttga aattacgctt caggataatc ccgctaactt gaggacgaac
     4261 ttctgcgatc cggtaggcac tggtgcgacc cggaagctcg gttgtgatct gcagaggttc
     4321 agttttgact gttactacgc caacggcggg catctgctgg ccaccttgtt gggcctgttt
     4381 gtcgtcacat cctgttaggg ctaagctgcc tgagagcatc agaacgaccg ccagaggcgt
     4441 aaaccctctg tttttgttca tatgtaaacc tcgagtgtcc gatttcaaat tggtcaatgg
     4501 tcaaaagtta ataaacccat tgctgcgttt atattatcgt cgtgctatgg tacatacatt
     4561 cacaaatgta tgtaaatcta acgcctgtaa attcacgaac atatggcacg aaaaaccaaa
     4621 caagaagcgc aagaaacgcg ccaacacatc ctcgatgtgg ctctacgtct tttctcacag
     4681 cagggggtat catccacctc gctgggtgag attgcaaaag cagctggcgt tacgcgcggt
     4741 gcaatctact ggcattttaa agacaagtcg gatttgttca gtgagatctg ggaactgtca
     4801 gaatccaata ttggtgaact agagcttgag tatcaggcaa aattccctgg cgatccactc
     4861 tcagtattaa gagagatatt aattcatgtt cttgaatcca cggtgacaga agaacggcgt
     4921 cgattattga tggagattat attccacaaa tgcgaatttg tcggagaaat ggctgttgtg
     4981 caacaggcac aacgtaatct ctgtctggaa agttatgacc gtatagaaca aacgttaaaa
     5041 cattgtattg aagcgaaaat gttgcctgcg gatttaatga cgcgtcgcgc agcaattatt
     5101 atgcgcggct atatttccgg cctgatggaa aactggctct ttgccccgca atcttttgat
     5161 cttaaaaaag aagcccgcga ttacgttgcc atcttactgg agatgtatct cctgtgcccc
     5221 acgcttcgta atcctgccac taacgaataa ccctgaatct gactccagga tttttcctgg
     5281 acattttcgt cgttgctatt ctggttcact gcgtcgtgat attcttgcgg tttgactttt
     5341 tcaggtcgtt cttcaggttc agaaaccttc attcatcatg actatgttcc agtattacaa
     5401 acgatcacgg cattttgttt tttcagcatt tattgctttt gtttttgtct tgttatgcca
     5461 gaacacggcg tttgcgcggg cgtcatcgaa tggtgatctg ccgacaaaag cggacctgca
     5521 ggcgcaactt gactcactaa ataaacaaaa agatctttct gctcaggaca aactggtgca
     5581 gcaggatctg acagatacat tagccaccct cgataaaatc gatcgcataa aagaagagac
     5641 agttcagcta cgacaaaaag tcgctgaagc gccggaaaaa atgcgccagg cgaccgcggc
     5701 gttaacagca cttagcgatg tcgataacga cgaagaaacg cgcaaaattc tgagtacgct
     5761 ttcgttgcgc cagctggaaa ctcgcgttgc ccaggcgctg gacgatttgc aaaacgcaca
     5821 aaacgatctg gcgtcttata acagccagct ggtttcgtta cagacgcagc ccgaacgcgt
     5881 gcaaaatgcg atgtataacg cttcgcagca gctgcaacaa attcgcagtc gtctggatgg
     5941 gactgatgtc ggcgagactg ccttacgtcc cagccagaaa gtgttaatgc aggctcagca
     6001 ggcgttgctg aatgcggaga ttgaccagca gcgtaaaagc ctggaaggga acaccgtctt
     6061 gcaggatacc ttgcaaaagc aatgtgatta cgtgacggcg aacagcgccc gtctggagca
     6121 ccagttgcag ctgttgcaag aagcggtaaa cagcaagcgc ctgactttaa ctgaaaaaac
     6181 ggcgcaggaa gccgtctccc cggatgaagc cgcgcgtatt caggctaatc cgctggtgaa
     6241 acaggaactg gaaattaacc agcagttaag tcagcgtctg attaccgcga ctgaaaacgg
     6301 taatcagttg atgcagcaaa acattaaagt caaaaactgg ctggagcggg cgctgcaatc
     6361 ggaacgcaat attaaagagc agattgccgt cctgaagggc agcctgctgt tgtctcgtat
     6421 cctttaccag caacaacaaa cgctgccctc ggcggatgaa ctggaaaaca tgaccaaccg
     6481 catcgcggat ttgcgtctcg aacagtttga agttaaccag cagcgtgatg cactcttcca
     6541 gagcgatgcg ttcgtcaaca aactggaaga aggtcacacc aacgaagtca acagcgaagt
     6601 tcacgatgcg ttattgcaag tggttgatat gcgtcgcgaa ttgctggatc aactcaacaa
     6661 acagttgggt aaccagctga tgatggccat taacctgcaa atcaaccagc agcagttaat
     6721 gagcgtatcg aaaaacctga aatccatcct gactcagcaa atcttttggg tgaacagtaa
     6781 ccgtccaatg gactgggact ggatcaaagc gttcccgcaa agcctgaaag atgaatttaa
     6841 gtcgatgaaa atcacggtga actgggaaaa agcctggcct gccgttttta tcgctttcct
     6901 cgccggtttg ccgctgctgt tgattgccgg gctgatccac tggcgtctgg gctggctgaa
     6961 agcgtatcaa cagaaactgg cttccgctgt gggttccctg cgtaacgata gccagctcaa
     7021 cacaccaaaa gcgatcctta tcgacctgat ccgcgcgctg ccggtgtgcc tgattattct
     7081 cgcggttggc ctgattctgt tgaccatgca gctcaacatc agcgaactgc tatggtcgtt
     7141 cagtaaaaaa ctggcgattt tctggctggt gtttggcctg tgctggaagg tactggagaa
     7201 aaatggcgtt gccgtgcgtc acttcggcat gccggagcag cagaccagcc actggcgtcg
     7261 gcaaattgtc cgcatcagtc tcgcgttgct gcctatccat ttctggtctg tggtggcaga
     7321 actttccccg ctgcatctga tggatgatgt gctggggcag gcgatgattt tcttcaacct
     7381 gctgctgatt gccttcctgg tatggccaat gtgccgcgaa agctggcgtg ataaagagtc
     7441 gcacaccatg cgactggtca ccattaccgt gctgtcgatt atcccgattg cattgatggt
     7501 gctgactgcc acgggttatt tctacaccac gctgcgcctc tccgggcgtt ggattgaaac
     7561 cgtttatctg gtgatcatct ggaacttgct ctaccagacg gtattgcgcg gcttaagcgt
     7621 agcggcgcgg cgtatcgcct ggcgtcgtgc gttggcgcgt cggcagaatc tggtgaaaga
     7681 gggcgcagaa ggtgccgaac cgccggaaga acccaccatt gcactggaac aggttaacca
     7741 gcagacgctg cgtattacca tgttgctgat gtttgcgctg ttcggtgtca tgttctgggc
     7801 aatttggtcc gatttgatca ccgtgttcag ctatctcgac agcatcacgc tctggcatta
     7861 caacggcact gaagctggcg ctgcggtggt gaaaaacgtc accatgggca gtctgttgtt
     7921 tgcgattatc gcctcaatgg tggcctgggc gttgattcgc aacctgcctg gtttactgga
     7981 agtgctggtg ctctcgcgac tgaatatgcg tcagggcgcg tcgtatgcca ttactaccat
     8041 ccttaactac atcattattg ccgttggtgc gatgacggtg ttcggatcgc tgggcgtctc
     8101 ttgggataaa ctccagtggc tggccgcagc attatccgta ggtcttggtt ttggtttaca
     8161 agaaattttc ggtaacttcg tctccggttt gatcattctg ttcgaacgtc cggtgcgtat
     8221 tggcgatacg gtaaccattg gtagcttctc ggggacggta agtaagatcc gtattcgtgc
     8281 gacaacgatt accgatttcg atcgcaaaga agtgatcatc ccgaacaaag cgtttgttac
     8341 cgagcgtctg atcaactggt cgttgactga cactactacg cgtctggtga tccgtctcgg
     8401 cgtggcctat ggctccgatc tggaaaaagt gcgtaaagtg ttactgaagg cggcgactga
     8461 gcacccaagg gtgatgcacg aaccaatgcc ggaagtcttc tttacggcgt ttggtgccag
     8521 cacgttggat catgagctgc gtctgtatgt gcgtgaactg cgtgaccgta gtcgtactgt
     8581 cgatgagctg aaccgtacta tcgatcagct gtgccgtgaa aacgacatca acattgcctt
     8641 taaccagctt gaagtgcatc tgcacaacga gaagggcgat gaggtgacgg aagtcaaacg
     8701 cgactacaaa ggcgatgacc cgacgccagc ggtagggtaa taaccaaagg gggcgacatt
     8761 tagttgcccc gagattactg acaaagtgcg cgttgttcat gccggatgcg gcgtgaccgc
     8821 cttatccggc ctacaaagtc atgcaaattc aatatattga agtttattgg tgatactgat
     8881 aagcgtagcg catcaggcag ttttgcgttt gcccacaatc ttaggggaca tttagcggcc
     8941 ccatttattt ctcacttttc cgcctcatca tcgcgcgtta atttcttttc ataatcgcgt
     9001 tttacaatat ccagcgcgcg cagcacggtg cgggctggga tctgattttc ttccagcagc
     9061 acaatcaaat cgacagccag tttgacatcg tcaggggcat tttccagtga catactctct
     9121 ccattgctag cgggttaagc gcgctagcct gttttcgatt ttttccagcg catggcggca
     9181 gcgtgccagg cgcgcttcat aggcttccac ttcacgatgc agcgtttgct gttccacgag
     9241 atcggtcacc cgcgccaggc gggctctgcg ttcggcaacc atctcacgta gccgccgctc
     9301 aaaatcctga tgctgaatac gtttacgctg ccagcgggca attttcggtg gcgcactgtc
     9361 ccactcgcgc aatgaccagg cggaggcttc acgcgcgatg gcttccagtt gtgccgccag
     9421 atgttccgcc agccaggcca cttgcggcag ctgttgctgc tcaactgcat gacgaagcgc
     9481 agccagatta tcgcccgcct catcgagaca agcctgcagt gttgtcgcac gggtttgaaa
     9541 aaggtgtctg tcaaaacggg cacttagcgt ggcaaattgc gctaccgggg cacaacgctg
     9601 acgcagcgta gcgagctgtc cttccagttt ttccagcagc agggcggttt tcacgataag
     9661 acctcaatga aaatgataat tgttatgcta aagtagccac tctgtaagct gacaacactc
     9721 aatatgcaac gaatcatttt aatcatcatt ggctggctgg cggtagtgct gggtacgctg
     9781 ggcgtggtat taccggtatt accgacgacg ccgtttatcc tgctggcggc ctggtgcttt
     9841 gcccgttctt ccccgcgctt tcacgcctgg ttgctgtacc gctcatggtt tggcagctat
     9901 ctacgtttct ggcagaaaca tcatgcgatg ccgcgcggcg tcaaaccgcg ggcgattttg
     9961 cttattttgc tcacgtttgc catttctctg tggttcgtcc agatgccatg ggtgcgcatc
    10021 atgttgctgg taattctcgc ctgtttgctt ttctatatgt ggcgaattcc ggtgattgat
    10081 gaaaagcaag aaaagcactg aagcacaaca atcgcagttg caattattgc gtacagccag
    10141 tacattctgg cgttttcgag cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt
    10201 tgatacgttt aaaacgcgcc gtgagtacca ccgtaacaag caggcataca cttatgaccg
    10261 cgactgcaca gcagcttgag tatctcaaaa atagcatcaa aagcattcag gactacccaa
    10321 aacccggcat tcttttccgc gatgtcacca gcttactgga agacccgaaa gcttacgctc
    10381 tcagcatcga cttgctggtt gagcgttaca aaaatgcggg cattaccaaa gttgtcggca
    10441 ccgaagcgcg tggcttcttg tttggcgctc cggtagctct gggtctgggc gttggctttg
    10501 taccggtccg taaaccgggc aaactgccgc gtgaaaccat cagtgaaact tacgacctgg
    10561 aatacggcac cgatcagctg gagatccacg ttgatgccat caaaccgggc gacaaagttc
    10621 tggtggtgga cgatctgctg gcaaccggcg gcactatcga agcgaccgtt aaactgatcc
    10681 gtcgtctggg tggtgaagtg gctgacgctg cgttcattat caacctgttc gatctcggcg
    10741 gcgaacagcg tctcgaaaaa cagggcatta ccagctacag ccttgtcccg ttcccgggcc
    10801 attaattatc gccagtcttg tgctgcccac gctacggaca gcacaagatg tgcattcagc
    10861 ctcgccgttc tgacggggct gtgttagcat taccccttcg tgaatccacc ttccagcgtt
    10921 tcagagcctg ccaatgagtt atcaggtctt agcccgaaaa tggcgcccac aaacctttgc
    10981 tgacgtcgtc ggccaggaac atgtgctgac cgcactggcg aacggcttgt cgttagggcg
    11041 tattcatcat gcttatcttt tttccggtac ccgtggcgtc ggaaaaacct ctatcgcccg
    11101 actgctggcg aaggggctaa actgcgaaac cggcattacc gcgacgccgt gcggcgtgtg
    11161 cgataactgt cgtgaaatcg agcaggggcg ctttgtcgat ctgattgaaa tcgacgccgc
    11221 ctcgcgcacc aaagttgaag atacccgcga cctgctggat aacgtccagt acgctccggc
    11281 gcgtggtcgt ttcaaagttt atctgatcga cgaagtgcat atgctgtcgc gccacagctt
    11341 taacgcactg ttaaaaaccc ttgaagagcc gccggagcac gttaagtttc tgctggcgac
    11401 gaccgatcca cagaaattgc cggtgacgat tttgtcgcgc tgtttgcaat ttcatctcaa
    11461 ggcgctggat gtcgagcaaa ttcgccatca acttgagcac atcctcaacg aagaacatat
    11521 cgctcacgag ccgcgggcgt tgcaattgct ggcgcgcgcc gctgaaggca gcctgcgaga
    11581 tgccttaagt ctgaccgacc aggcgattgc cagcggtgac ggccaggttt caacccaggc
    11641 agtcagtgcg atgctgggta cgcttgacga cgatcaggcg ctgtcgctgg ttgaagcgat
    11701 ggtcgaggcc aacggcgagc gcgtaatggc gctgattaat gaagccgctg cccgtggtat
    11761 cgagtgggaa gcgttgctgg tggaaatgct cggcctgttg catcgtattg cgatggtaca
    11821 actttcgcct gctgcacttg gcaacgacat ggccgccatt gagctgcgga tgcgtgaact
    11881 ggcgcgcacc ataccgccga cggatattca gctttactat cagacgctgt tgattggtcg
    11941 caaagaatta ccgtatgcgc cggaccgccg catgggcgtt gagatgacgc tgctgcgcgc
    12001 gctggcgttc catccacgta tgcctctgcc tgagccagaa gtgccacgcc agtcctttgc
    12061 gcccgtcgcg ccaacggcag taatgacgcc aacccaggtg ccgccgcaac cgcaatcagc
    12121 gccgcagcag gcaccgactg taccgctccc ggaaaccacc agccaggtgc tggcggcgcg
    12181 ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa aagagtgaac cggcagccgc
    12241 tacccgcgcg cggccggtga ataacgctgc gctggaaaga ctggcttcgg tcaccgatcg
    12301 cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg ccagccaaaa aagaagcgta
    12361 tcgctggaag gctaccactc cagtgatgca gcaaaaagaa gtggtcgcca cgccgaaggc
    12421 gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg gcggcgaagc tggcggcaga
    12481 agccattgag cgcgacgcgt gggcggcaca ggtgagtcaa ctttcgctac caaaactggt
    12541 cgaacaggtg gccttaaatg cctggaaaga ggagagcgac aacgcagtat gtctgcattt
    12601 gcgctcctct cagcggcatt tgaacaaccg cggtgcacag caaaaactgg ctgaagcgtt
    12661 gagcacgtta aaaggttcaa cggttgaact gactatcgtt gaagatgata atcccgcggt
    12721 gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa aaacttgcgc aggcgcgcga
    12781 gtccattatt gcggataata atattcagac cctgcgtcgg ttcttcgatg cggagctgga
    12841 tgaagaaagt atccgcccca tttgatcgta agcacagctt acgttcgtca tccttaacgt
    12901 gattgagaga gaaacctatg tttggtaaag gcggtctggg taacctgatg aagcaagccc
    12961 agcagatgca agaaaaaatg cagaaaatgc aggaagagat cgcgcagctg gaagtcaccg
    13021 gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg tgcacacaac tgccgtcgcg
    13081 tagagatcga cccgagcctg ctggaagacg acaaagagat gctggaagac ctggtggctg
    13141 cagcattcaa cgacgcagca cgtcgtattg aagaaacgca gaaagaaaaa atggcctctg
    13201 tatcctccgg aatgcagctg ccgcctggct ttaagatgcc gttctgatgc aaaccagccc
    13261 gctgttaaca cagcttatgg aagcactgcg ctgtctgccg ggcgttggcc cgaagtcggc
    13321 gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc ggcgggatgc gtctggcgca
    13381 ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc gattgccgca ctttcaccga
    13441 acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag gaaaacggtc aaatctgtgt
    13501 ggtggagagt ccggcggaca tctacgccat tgagcagacg gggcagtttt caggccgtta
    13561 ttttgtgttg atggggcacc tgtcaccgct ggacggcatc ggtccggatg atatcggcct
    13621 tgatcgtctg gaacagcgtc tggcagagga aaaaatcact gaagtgatcc tcgccaccaa
    13681 tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc gagctttgcg cgcaatatga
    13741 cgtggaagcc agccgaatcg ctcatggcgt accggttggc ggcgaactgg aaatggtcga
    13801 tggcaccacg ctgtcacact cccttgccgg gcgtcataag attcgttttt aagcaaacga
    13861 gagcaggatc acctgctctc gcttgaaatt attctccctt gtccccatct ctccctcatc
    13921 ctgtttttaa ccttaaaatg gcattattga ggtagaccta catgaaagga caagaaactc
    13981 gtggttttca gtcagaagtg aaacagcttc tgcacctgat gatccattct ctctattcca
    14041 ataaagaaat cttcctgcgt gagcttatct ctaacgcctc cgatgcggcg gacaagctgc
    14101 gtttccgtgc gctatctaac ccggacctgt acgaaggtga tggcgaactg cgcgttcgtg
    14161 tctctttcga taaagacaag cgcacgctga ccatctccga taacggcgtg gggatgaccc
    14221 gcgacgaagt gattgaccat ctggggacta tcgctaaatc cggtaccaaa tcattcctcg
    14281 aatccctggg ttctgaccag gcgaaagaca gccagctgat cggtcagttt ggtgttggtt
    14341 tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg tactcgcgcg gcaggcgaaa
    14401 aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg tgaatatacc gttgccgaca
    14461 tcaccaaaga agatcgtggt actgaaatca ccctgcatct gcgtgaaggc gaagacgagt
    14521 tcctcgatga ctggcgcgtg cgttccatca tcagcaaata ctccgaccat atcgcgctgc
    14581 cggtagagat cgaaaaacgc gaagagaaag acggcgaaac cgttatctcc tgggagaaaa
    14641 tcaacaaagc gcaggcgctg tggactcgta acaagtcgga aatcaccgat gaagagtaca
    14701 aagagttcta caaacacatc gcccacgact ttaatgatcc gctgacctgg agccacaacc
    14761 gcgttgaagg taagcaggag tacaccagcc tgctgtacat cccgtcccag gctccgtggg
    14821 atatgtggaa ccgtgatcat aaacacggcc tgaaactgta cgtccagcgt gtgttcatca
    14881 tggacgacgc agaacagttc atgccgaact atctgcgctt cgtgcgtggt ctgattgact
    14941 ccagcgatct gccgctgaac gtttcccgtg aaatcctcca ggacagcacg gtaacgcgta
    15001 acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct ggaaaaactg gcgaaagacg
    15061 acgcggacaa ataccagacc ttctggcaac agtttggcct ggtactgaaa gaaggtccgg
    15121 cggaagattt cgctaaccag gaagcgatcg ccaaactgct gcgttttgct tctacttata
    15181 ccgattcttc tgcgcagacc gtgtctctgg aagactacgt ttcccgcatg aaagaagggc
    15241 aggagaaaat ctactacatc actgcagaca gctatgcggc agcgaagagc agcccgcacc
    15301 tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct ttccgaccgc atcgatgagt
    15361 ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt ccagtcggtg tctaaagttg
    15421 acgagtcgct ggaaaaactg gctgacgaag ttgatgagag cgcgaaagaa gcggagaaag
    15481 cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg cgagcgcgtg aaagatgtcc
    15541 gtctgactca ccgtctgacc gatacgccag cgatcgtttc gaccgacgcg gacgaaatga
    15601 gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa agtgccagaa gtgaaataca
    15661 tcttcgaact gaacccggat cacgtactgg tgaaacgtgc ggcagatact gaagatgaag
    15721 cgaagttcag cgagtgggta gaactgctgc tggatcaggc gctgctggca gaacgcggca
    15781 cgctggaaga tccgaacctg tttattcgtc gtatgaacca gctgctggtt tcctgatgtc
    15841 atgccggatg acctttgtgt catccggcat ttttcttttc atcatctgca ctttccgcaa
    15901 attatctcgc cattaaccgt ttcagcctca ggtgcctttc ttgaggcaat cgcctgttgg
    15961 tggtatcgtt tatcgctttt tcaaaaaatt cgacacattt taaggggatt ttcgcaatgc
    16021 gtatcattct gcttggcgct ccgggcgcgg ggaaagggac tcaggctcag ttcatcatgg
    16081 agaaatatgg tattccgcaa atctccactg gcgatatgct gcgtgctgcg gtcaaatctg
    16141 gctccgagct gggtaaacaa gcaaaagaca ttatggatgc tggcaaattg gtcaccgacg
    16201 aactggtgat cgcgctggtt aaagagcgca ttgctcagga agactgccgt aatggtttcc
    16261 tgttggacgg cttcccgcgt accattccgc aggcagacgc gatgaaagaa gcgggcatca
    16321 atgttgatta cgttctggaa ttcgacgtac cggacgaact gattgttgat cgtatcgtag
    16381 gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt taaattcaat ccgccgaaag
    16441 tagaaggcaa agacgacgtt accggtgaag aactgactac ccgtaaagac gatcaggaag
    16501 aaaccgtgcg taaacgtctg gttgaatacc atcagatgac tgcaccgctg atcggctact
    16561 actccaaaga agcggaagcg ggtaacacca aatacgcgaa agttgacggc accaagccgg
    16621 ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta attcgaaagc gcgcacggac
    16681 agtcccctcg ccccctcggg gagagggtta gggtgagggg aacaggcccg cacaagcaaa
    16741 cttatcagca atctcaggcc ggatattcat tcggcctttt acaaaaacct caatctgcgc
    16801 tattcctttt tctgatttga cctctcacaa caattagttc ttcttcctca cttttccgct
    16861 acaattatca acaagttgaa tcgataagag gcggtaatgc gtcagactaa aaccggtatc
    16921 ctgctggcaa acctgggtac gcccgatgcc cccacacctg aagcggtaaa acgctatctg
    16981 aaacaatttt taagcgacag acgcgtggtt gatacctcac ggttgttatg gtggccgttg
    17041 ctgcgcggcg tgattttgcc gctgcgctcg ccgcgtgtgg cgaagatgta cgcctcggtc
    17101 tggatggaag acggctcgcc gctgatggtt tacagccgcc agcaacagca ggcgttggca
    17161 caacgtttac cggatacgcc cgtagcgctg ggcatgagct acggctcgcc atcactggaa
    17221 agcgccgtcg atgaactcct ggcagagcat gtagatcata ttgtggtgct gccgctttat
    17281 ccgcaattct cctgttctac ggtcggtgcg gtatgggatg aactggcacg cattctggcg
    17341 cgcaaacgta gcattccggg gatatcgttt attcgtgatt acgccgataa ccacgattac
    17401 attaatgcac tggcgaacag cgtacgcgct tcttttgcca aacatggcga accggatctg
    17461 ctgctgctct cttatcatgg cattccccag cgttatgcag atgaaggcga tgattacccg
    17521 caacgttgcc gcacaacgac tcgcgaactg gcttccgcac tggggatggc accggaaaaa
    17581 gtgatgatga cctttcagtc gcgctttggc cgggaaccct ggctgatgcc ttataccgac
    17641 gaaacgctga aaatgctcgg agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc
    17701 tttgctgcgg attgtctgga gacgctggaa gagattgccg agcaaaaccg tgaggtcttc
    17761 ctcggtgccg gcgggaaaaa atatgaatat attccagcgc ttaatgccac gccggaacat
    17821 atcgaaatga tggctaatct tgttgccgcg tatcgctaaa gctgagcggt aaagaactga
    17881 gcgccgtcgc gaagagcctc gtcggcggtt ttcatcattc gtgaataatg caaaaaggca
    17941 tgcagcgtac ctgggtagag tttgaactca cagggctgct gatgcgccgc taacgtctgg
    18001 taaagcagac ggctgtcatc cagcagcgga tcgaactccg ccccggcaat aaaacagggc
    18061 ggaacttcgc gagtgagatc attattaaac agacagtaat acggtgactc gcggtccgcg
    18121 tcgttgctta aatacgcctc ttcatacatc tgcaaatcct gttgcgttaa gccatcccag
    18181 acaccgccca acagacgacg agtcacggaa tcccgtaatc cgtaaagccc ataccacagc
    18241 aaaacacccg caattttacc gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg
    18301 agcgccagca tggcacctgc ggaatcaccg gcaaagccaa tgcgggacat attgatttga
    18361 taatcctccg cctgctggtg gaaataacaa caagcagcca caatttcctc tatcgcttgc
    18421 ggaaaacgcg cttcaggtga aagggggtaa tcaataccaa tcaccgtaca ttggctgtag
    18481 cttgccagca ggcgcatgat gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct
    18541 ccatgcaaat aaaatagcgt cgccgggcta tctggctgcg gacaaaagag acgtgtttcc
    18601 acctgcccat attttgttgg aaccatgtaa gctctggttg ccatttctgg agcgcccgca
    18661 ttccagaatc ggcgctcaag cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg
    18721 ggccaggacg gtaaatccgg ctgaagagta ttcacaacgg tcttcatttc agcagaaata
    18781 aggtccagaa caggtagttt gttttccggc ttcatacaaa aactcctttc aaattacgtc
    18841 attgtaagga accactgcca tgaaaatgcg atcccgcctg ctgatattga aactggctgc
    18901 gtctcgcgcg ctcccgtcag attgtgttaa cattcgccgc tcagttaacc acccgtaaaa
    18961 acaaccatga aatttcccgg taaacgtaaa tccaaacatt acttccctgt aaatgcacgc
    19021 gatccgctgc ttcagcaatt ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt
    19081 attgaccaga cgctggtcga tattgaagcg aaagtggatg atgaattcat tgagcgttat
    19141 ggattaagcg ccgggcattc actggtgatt gaggatgacg tagccgaagc gctttatcag
    19201 gagctaaaac agaaaaacct gattacccat cagtttgcgg gtggcaccat tggtaacacc
    19261 atgcacaact actcggtgct cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc
    19321 aatattgaaa ttggcagtta tgcctatcgt tacctgtgta acacttccag ccgtaccgat
    19381 cttaactatc tacaaggcgt ggatggcccg attggtcgtt gctttacgct gattggcgag
    19441 tccggggaac gtacctttgc tatcagtcca ggccacatga accagctgcg ggctgaaagc
    19501 attccggaag atgtgattgc cggagcctcg gcactggttc tcacctcata tctggtgcgt
    19561 tgcaagccgg gtgaacccat gccggaagca accatgaaag ccattgagta cgcgaagaaa
    19621 tataacgtac cggtggtgct gacgctgggc accaagtttg tcattgccga gaatccgcag
    19681 tggtggcagc aattcctcaa agaccacgtt tctatccttg cgatgaacga agatgaagcc
    19741 gaagcgttga ccggagaaag cgatccgttg ttggcatctg acaaggcgct ggactgggta
    19801 gatctggtgc tgtgcaccgc cgggccaatc ggcttgtata tggcgggctt taccgaagac
    19861 gaagcgaaac gtaaaaccca gcatccgctg ctgccgggcg ctatagcgga attcaaccag
    19921 tatgagttta gccgcgccat gcgccacaag gattgccaga atccgctgcg tgtatattcg
    19981 cacattgcgc cgtacatggg cgggccggaa aaaatcatga acactaatgg agcgggggat
    20041 ggcgcattgg cagcgttgct gcatgacatt accgccaaca gctaccatcg tagcaacgta
    20101 ccaaactcca gcaaacataa attcacctgg ttaacttatt catcgttagc gcaggtgtgt
    20161 aaatatgcta accgtgtgag ctatcaggta ctgaaccagc attcacctcg tttaacgcgc
    20221 ggcttgccgg agcgtgaaga cagcctggaa gagtcttact gggatcgtta agttatcgtc
    20281 ggttcgtagg ccagataagg cgttcacgcc gcatctggca tttggctctc gatgcctgat
    20341 gcgacgctgg cgcgtcttat catgcctaca tatttttcat attttacatc cggcaaccac
    20401 cgtttacccc gtcaccacct cacccgccgg tggcgtttcc agcagttcca gcatggtacg
    20461 ggcgatttca cgctcgccca tcactacctg attcgcacca cgttcggtga tatacgccac
    20521 ttcatcgtca taatgggcgc gggcaataat ctcaatatcc ggatttttcg cgcgggcaga
    20581 tgccacaatc tcacccgctt cataaccgtt gggaatcgtc aggatcagcc attttgcaca
    20641 ttccagatgc gccagttgca taatttcttc gttcgccgca ttgcccaata ctgcgcggac
    20701 cccgcgctct cgcagctcat caacacgggt tcgtgacgtc tcaatcacca ccagcggaat
    20761 atcagaggcg agcaatttct cccccagcag gctgcctaca cgaccgtaac ccaccagtag
    20821 cgcatggttg caaatatcca ctgggatctg cttctcttct tcgattgcct cttccagcgt
    20881 ctgctcttcc agcgtttcgg tcttcgccag atatttctcc agtagtgcga acagtaccgg
    20941 gttgagcata atcgacagga tcgcccctgc cagtactagg ttttgtccgg cctgcggcag
    21001 taaattcaat gccattccca gtcccgccag gataaacgcg aactcaccaa tctgcgccag
    21061 gctggcggcg atggttaatg ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa
    21121 tgcggctaac gacttaccaa acagaataat cgccagcgtc gccagcactg ccagcggttg
    21181 ctgaatcaga attaacggat caaacaacat cccgacggag acaaaaaaca gcaccgcaaa
    21241 cgcgtcgcgc aatggcagcg tatcgtgggc ggcacggtga ctcagttcag actcgttcag
    21301 taccatcccg gcaaagaacg caccgagtgc aaaggagaca tcaaacagct ctaccgcacc
    21361 aaaggcaatc cctaacgcca gcgccagcac cgacagggta aacagctcgc gagaaccggt
    21421 tgccgcgctg cgtgccataa tccacggcac cagacggcga cctaccagca tcataatggc
    21481 gataaatgcg atcactttgc cgatggtgat ccccatatcg actgcaagag tggcaaagcc
    21541 cacatcgccc tgttccatca ttcctgccac tgcgggcagc aacaccagcg tcagaaccat
    21601 taccaggtct tccacaatca accaaccgat ggcgatttgc ccacgctgac tgtcaattaa
    21661 ttgccgttct tcaagtgcgc gcagtaacac cacggtactg gcggtggaaa gacataaacc
    21721 gaacacgata ccggtcatta acgaccagcc cagcacggcg gagagcgcca tacccagcag
    21781 cgtcgccacg gctatctggg cgatcgcgcc gggaatggcg atggacttta ccgccatcaa
    21841 atccttgagc gaaaaatgca aaccgacgcc gaacatcagc aaaataacgc ccagttcagc
    21901 cagttccggc gcaagcttgg tatcggcaac aaagcccgga gtgaatggtc ctgccagcac
    21961 acccgctaac agatatccca ccagaggaga aatacgtagt ttattggcca gcatgccgag
    22021 gataaaggcg agcacaaggc cgccaacaat ggtggtgata agcggggtgg cgtgatgcat
    22081 tccgtctcct tttcctggtg gttattgtcc atttttggcc gggaaaacca aaattacagg
    22141 taatagttta tgacaatttc attgatgatg ttcatgaata attattgaat tttgcagaaa
    22201 aatggaatta gctgcaaaaa aagcacggat cggaaaacag aaggcggttt aacagaggaa
    22261 aggcttatgg cgactgtaaa acgatgcagc caaagtttgc ctttggctgc aatgaaatca
    22321 gtctttatgc cggttatcag gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc
    22381 acagatttta tagactaact cgatactggt gtgatcggcg ataagcccca gaactgccgc
    22441 tcccagacct cccataccaa aagcaaaacc gaaaaagagt ccagaaacca taccgatacg
    22501 tcccggaagc agctcctgag cgtaaaccag aatggcagag aatgccgaag cgaggataaa
    22561 tccaataatc accgttaaaa cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt
    22621 aaacggcgca acgccgagga tagagcccca aatcacatat ttccgcccaa ttttatcccc
    22681 tacaggcccg ccgatcaccg tacctgccgc aacggcaaac aggaaggcaa acagatgaag
    22741 ctgagcattc tggatagata atccgaattt ttgcatcaga taaaaggtgt aatagctgct
    22801 gatgctcgcc atatagaaat atttcgagaa aatgaggatt aacagaatgc tgaccgccag
    22861 taccactttg ttgcgcggca gtggattgat aatcgtcgct ttgggttttc ctttattcat
    22921 tcggtgctgt gccgagtacc aacggctgat ttgcgccaac accacgatcg ccagcagtgc
    22981 cgcaagcaca aaccaggcaa cgttgccttt gccataaggc gcgataatca ccgccgccag
    23041 caagggtccc agggaactgc caaagttgcc gccgacctga aagatagatt gcgccaggcc
    23101 atgccgcccg ccggaagcca tacgggctac gcgagaagat tccggatgaa agaccgatga
    23161 accggtaccg accagcgccg ccgccagcag aactgcgcca aaactgcccg ccagcgcaag
    23221 cagcaccaga ccacttaagg taaagcacat gccaattggc aacgaccatg gcatcggata
    23281 tttatcggtc cagtagccga ccactggttg cagtagcgaa gaggcgagct ggaaggtgag
    23341 ggttatcatg ccaatctgca taaatgtcag agaaaattct gactgaagca gcggataaat
    23401 cgccagaatc agcgattgga tcatgtcgtt cagcagatgt gagaggctga tagcacctaa
    23461 aataccaaac gatgttcggg ccttggtcgt tgacgcagcc gcgcccgcca caggctgggg
    23521 ttgttcactc attgccatag gaaagtcact ttttcagggt tgcgatgtaa agaatgatct
    23581 tatttgtgat tattacccga ctaacatacc tgtatgcgtc gcctgaagga agtctcaacg
    23641 ccgaatacag aatttctaat ctggatgcag atttatcttc accggacgca gacttgtcta
    23701 tgatgtcgcg tcatactatt tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa
    23761 attattgcag cggggcgtgg cgttagcgct gttaaccaca tttacactgg cgagtgaaac
    23821 tgctctggcg tatgagcagg ataaaaccta caaaattaca gttctgcata ccaatgatca
    23881 tcatgggcat ttttggcgca atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct
    23941 ggtggatggt atccgcaaag aggttgcggc tgaaggcggt agcgtgctgc tactttccgg
    24001 tggcgacatt aacactggcg tgcccgagtc tgacttacag gatgccgaac ctgattttcg
    24061 cggtatgaat ctggtgggct atgacgcgat ggcgatcggt aatcatgaat ttgataatcc
    24121 gctcaccgta ttacgccagc aggaaaagtg ggccaagttc ccgttgcttt ccgcgaatat
    24181 ctaccagaaa agtactggcg agcgcctgtt taaaccgtgg gcgctgttta agcgtcagga
    24241 tctgaaaatt gccgtcattg ggctgacaac cgatgacaca gcaaaaattg gtaacccgga
    24301 atacttcact gatgtcgaat ttcgtaagcc cgccgatgaa gcgaagctgg tgattcagga
    24361 gctgcaacag acagaaaagc cagacattat tatcgcggcg acccatatgg ggcattacga
    24421 taatggtgag cacggctcta acgcaccggg cgatgtggag atggcacgcg cgctgcctgc
    24481 cggatcgctg gcgatgatcg tcggtggtca ctcgcaagat ccggtctgca tggcggcaga
    24541 aaacaaaaaa caggtcgatt acgtgccggg tacgccatgc aagccagatc aacaaaacgg
    24601 catctggatt gtgcaggcgc atgagtgggg caaatacgtg ggacgggctg attttgagtt
    24661 tcgtaatggc aaaatgaaaa tggttaacta ccagctgatt ccggtgaacc tgaagaagaa
    24721 agtgacctgg gaagacggga aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa
    24781 ccagcaaatg atctcgctgt tatcaccgtt ccagaacaaa ggcaaagcgc agctggaagt
    24841 gaaaataggc gaaaccaatg gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca
    24901 gaccaatatg gggcggttga ttctggcagc ccaaatggat cgcactggtg ccgactttgc
    24961 ggtgatgagc ggaggcggaa ttcgtgattc tatcgaagca ggcgatatca gctataaaaa
    25021 cgtgctgaaa gtgcagccat tcggcaatgt ggtggtgtat gccgacatga ccggtaaaga
    25081 ggtgattgat tacctgaccg ccgtcgcgca gatgaagcca gattcaggtg cctacccgca
    25141 atttgccaac gttagctttg tggcgaaaga cggcagactg aacgacctta aaatcaaagg
    25201 cgaaccggtc gatccggcga aaacttaccg tatggcgaca ttaaacttca atgccaccgg
    25261 cggtgatgga tatccgcgcc ttgataacaa accgggctat gtgaataccg gctttattga
    25321 tgccgaagtg ctgaaagcgt atatccagaa aagctcgccg ctggatgtga gtgtttatga
    25381 accgaaaggt gaggtgagct ggcagtaatc cgaaagtgcc ggatgtttgc atccggcaca
    25441 atgcttaatc gcggcgggcg atatcagcaa atttggcatc gaggatcttt gccagatcgc
    25501 ctgccgccag ttcgatatcc agtccgcgct tgccgccgga aacataaata gtggcaaatt
    25561 cttgtgcggg ggcgtcgata atcgttggca gacgtttttt ctgccccagt gggctaatcc
    25621 ccccaaccag gtatcccgtc gaacgctgcg cgaccatcgg atcggccatc tcaactttct
    25681 tggcacccag cgcttttgct acttttttaa gatccagttg accggcgacc ggcgtaacgg
    25741 ccacggcaag gtgtttcata tcaccgttca ctgccaccag cagcgttttg tagacctgat
    25801 ccggattcaa acctaatttt ttgacgactt catcgccaaa attggtttca gccggatcgt
    25861 gctcgtaggt atggatttga aacgaaatct tgtttttttc gagtaattta actgcgggtg
    25921 tcatggtaat tttcctgcct taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc
    25981 caaaaaaaag agtcatcttg cctaagagta ttggcaggat ggtgagattg agcgacaatc
    26041 gagttacacc gtcgcttaaa gtgacggcat aataataaaa aaatgaaatt cctctttgac
    26101 gggccaatag cgatattggc cattttttta gcgcaacatt tgcggtaaat tcccttctcc
    26161 atacaggtgt agtgcaccga ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt
    26221 atcccgccag gcgagatttc gctgatgcat cagcacatcg tacagcgact gactgaacgt
    26281 attgggcagc gttatttcat tattttgcgg cggtgcattc agccaccagc tcatcatttg
    26341 ttgcagcaac cgtgcattgg tgtgccagtg ggtcagtgta tcgtccagca gcgccagtcc
    26401 tttgtcaggg agctggagca acatggcaat ctggttttca gccccttcca gttcaatcac
    26461 gggtttatgt tgttgcttcg ccgcctgcaa tagctggtaa tcgataccgt attctgcccg
    26521 cagccccagt ttttgtgcct gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg
    26581 ggtagaaaag agtgagggag aaatgcccat ctcctggcta atgtgctgca gattttggag
    26641 ttgctcctcg ctaatgcgct cttccagcgc ctcgcaggca ggcaaattgg caaaaggcgt
    26701 atcgctggtg gaaacatccg cctcgacgat cagcgcatcg gcgtttttga gctttttgag
    26761 caaacgggtg ggcaggggag ccatatcgtg gctacccata tgaatactgc caatcagatg
    26821 aaaatggcga ttgccgggaa gggtgatatc gatggcaggc caggtgtaat gattaccgcg
    26881 cagcgcggcc caaagcgttt ttacccggta caacagatcc atacgacctc cctttgtgaa
    26941 atatcatgct agcgcgcggg gagggatggt gcaaatggag ttggggactt gtttaatgtg
    27001 tttgtatgat tcagtatatt cttgtatcgc tattcacagg gaagcaacag ttaaaaacta
    27061 tgaaacaggc aacaagaaaa ccgacgacac ccggagatat tcttctctat gaatatctgg
    27121 taccgctcga tttgaaaatc aatgagttag cagagttgct gcatgttcat cgtaatagcg
    27181 tcagtgcact gatcaataac aatcgtaaac tcaccactga gatggcattc cgtctggcga
    27241 aagtttttga taccacagtc gatttttggc taaacctcca ggcggcagtt gatctttggg
    27301 aagttgaaaa caacatgcgc acccaggaag aattgggacg gattgaaaca gtggctgaat
    27361 acttggcacg ccgtgaagag cgtgcaaaaa aggtcgcgta aggccaaaac atcgcttcat
    27421 ctcgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga tgccaaatgt
    27481 gccaccctaa agcagcgcat ccgcaatgac atacttattc cttcggttta aaccgcagca
    27541 accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct ccggcaacta
    27601 ccgggttaag cagtgttcca gtaaacggcc acaaaatacc ggcggcgacc ggaataccga
    27661 tactgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg
    27721 aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta atcgccgcgg
    27781 tttcaatggc aacatcactg ccgcccccca tcgcaatacc gacatccgcc tgggccagcg
    27841 ctggcgcatc gttaatgccg tcgcccacca ttgccacctg acgtccttca ctttgcaggc
    27901 gtttaatcgc ttcggcttta ccgtccggca gcaccccggc gatcacttca tcaatcccgg
    27961 cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc accagacgat
    28021 atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaat ggatcgcgta
    28081 ctgccagcag ggctaccgct ttcccgtcaa ccgccagcag cacaggcgtt gccccttgcg
    28141 atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc tcatttaaca
    28201 gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg cccagcccgc
    28261 gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta tcgaggatcg
    28321 cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc aatgcctgcg
    28381 cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct tcggtcagcg
    28441 tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt
    28501 cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc gacatcggcg
    28561 tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca atcaccaggg
    28621 tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg accagcgcaa
    28681 tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga ccgatttctg
    28741 gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg gtatggctgc
    28801 caaccgcact ggcacgaaac agcacgctac cgtcctgtac cactgtcccg gcatggacgc
    28861 tttcgccttc gcctttttgc tgcgggattg gttcgcccgt cagcatcgct tcatccagcc
    28921 atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca
    28981 gcaacatacc tggctgcact tccgccagag gcacgctttt ttcaccttcg tcagtaacca
    29041 ggcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa gaacgctgac
    29101 gtgcgcgtgc ttccagcata tggccgagat tgatcagacc gataatcatc gcgctggctt
    29161 cgtaataaag atgtcgcgct tccatcggga accactgcgg ccacaggttg acgctcatcg
    29221 aatagagcca cgccacgcca gtacccagcg ccaccagcgt atccatcgtc gctgcaccgt
    29281 tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc atcaccgcca
    29341 gggttatcag gccgataacc aaccacaggc tgcggttgtc agcggtgacc atcatgttat
    29401 cgccaatcat cccccagacc atcaccggga tacccaccgc cagtgcgaca attgcctgcc
    29461 agcggaagcg cttcatcgta gcaacggcgg tttcctgctg gcgctcacgg cgtttcgcgt
    29521 catcttcaat cgcttccgcg ccgtagcccg ctttttccac cgcctgcact aaatcttgtg
    29581 gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt gcctgagtga
    29641 cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg cagctcatgc
    29701 cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga agcgcctcag
    29761 aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta gcctttgggt
    29821 ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt tctgcactgg
    29881 cagtcccggt aacgtgcgct tcagtgatag acacatccgc ttgttccaca tccgggcgct
    29941 gttcgagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg tccagggtca
    30001 ggtcgataat ttgtgacata aaacactcct ttaagacagt tttgactggc tgtgataaag
    30061 gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcgag taaaagtccg
    30121 taaagattaa aaaattggct caatttgcat caggattaga catttatctc tttgttttcc
    30181 tgtagttaag ttgcgggtgc taagttaaat caagatgcct gaaaatcggc accggggtga
    30241 ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg caaacaaatt
    30301 acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg gacaaaatgc
    30361 cgactacatt ccctttctgg cgaatgtacc tagtcaactg gcagcagtgg ctattgtgac
    30421 ctgcgatggc aacatctatc gcgcgggtga tagtgattac cgctttgcgc tggaatccat
    30481 ctcgaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg cggtacagga
    30541 caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct tagagttgca
    30601 tggcggcaaa ccactgtcgc cactggtaaa tgctggcgct attgccacca ccagcctgat
    30661 taacgctgaa aatattgaac aacgctggca gcgaatttta catatccaac agcaactggc
    30721 tggtgagcag gtagcgctct ctgatgaagt caaccagtcg gaacaaacaa ccaacttcca
    30781 taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg atgcaatgga
    30841 agcctgtgac gtgtacaccc gtcagtgctc cacgctcatc aatactgttg aactggcaac
    30901 gcttggcgcg acgctggcgg cgggtggtgt gaatccgctg acgcataaac gcgttcttca
    30961 ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt atggtcgctc
    31021 cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct
    31081 ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg acgaagaagg
    31141 caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg gctataacgt
    31201 gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc tctcggtcta
    31261 tggaacgtcg tttccatcgg cattggg
//
LOCUS       DAZKGS010000042        30997 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKGS010000042 DAZKGS010000000
VERSION     DAZKGS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHOQY_001857"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHOQY_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9677975.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHOQY_001858"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHOQY_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9677976.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHOQY_001859"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHOQY_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9677977.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOQY_001860"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOQY_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9677978.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOQY_001861"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOQY_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9677979.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESMDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOQY_001862"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOQY_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9677980.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOQY_001863"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOQY_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9677981.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOQY_001864"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOQY_001864"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9677982.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOQY_001865"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOQY_001865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9677983.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHOQY_001866"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHOQY_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFP9677984.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHOQY_001867"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHOQY_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9677985.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHOQY_001868"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHOQY_001868"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9677986.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHOQY_001869"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHOQY_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9677987.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHOQY_001870"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHOQY_001870"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9677988.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHOQY_001871"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHOQY_001871"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9677989.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHOQY_001872"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHOQY_001872"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9677990.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHOQY_001873"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHOQY_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9677991.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHOQY_001874"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHOQY_001874"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9677992.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHOQY_001875"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHOQY_001875"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9677993.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHOQY_001876"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHOQY_001876"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9677994.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHOQY_001877"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHOQY_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9677995.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHOQY_001878"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHOQY_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9677996.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHOQY_001879"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHOQY_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9677997.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHOQY_001880"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHOQY_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9677998.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHOQY_001881"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHOQY_001881"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9677999.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHOQY_001882"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHOQY_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9678000.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHOQY_001883"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHOQY_001883"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9678001.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHOQY_001884"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHOQY_001884"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9678002.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7302 a         8042 c         8481 g         7172 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcatggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgata tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt acgcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga ttccgggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtta ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgcagagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgctatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcaggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag tggctggaag cgatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggat atcgccgatg cgcgccgccg
    21661 cgtgggcaat aaagtcgcgt tgcagggcaa tatggatccg tcgatgctgt acgcgccgcc
    21721 agcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc cccgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgcattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgatagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctgc tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatcccg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctagtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aggccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg agcgggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 agagatggac ggcattgcca cgctaaaaga gatcaaagcg ttaaatccgg cgattccggt
    26641 actgattatg accgcatact ccagcgtcga gacggcggta gaggcactga aaaccggggc
    26701 gctggattat ctcatcaagc cgctggattt cgacaccctg caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacgcctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgtggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct agccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg gggcaaagtc aggatattca
    27601 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgtgcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    27961 gatcacgtca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact ctgacgtttt ctcgtccagt ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc taccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaataa ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccacattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZKGS010000043        30370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKGS010000043 DAZKGS010000000
VERSION     DAZKGS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..905)
                     /gene="bacA"
                     /locus_tag="ACHOQY_001885"
     CDS             complement(84..905)
                     /gene="bacA"
                     /locus_tag="ACHOQY_001885"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9678003.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(995..1363)
                     /gene="folB"
                     /locus_tag="ACHOQY_001886"
     CDS             complement(995..1363)
                     /gene="folB"
                     /locus_tag="ACHOQY_001886"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9678004.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1468..2085
                     /gene="plsY"
                     /locus_tag="ACHOQY_001887"
     CDS             1468..2085
                     /gene="plsY"
                     /locus_tag="ACHOQY_001887"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9678005.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2098..3030)
                     /gene="ttdR"
                     /locus_tag="ACHOQY_001888"
                     /gene_synonym="dan"
     CDS             complement(2098..3030)
                     /gene="ttdR"
                     /locus_tag="ACHOQY_001888"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9678006.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3237..4148
                     /gene="ttdA"
                     /locus_tag="ACHOQY_001889"
     CDS             3237..4148
                     /gene="ttdA"
                     /locus_tag="ACHOQY_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9678007.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4145..4750
                     /gene="ttdB"
                     /locus_tag="ACHOQY_001890"
     CDS             4145..4750
                     /gene="ttdB"
                     /locus_tag="ACHOQY_001890"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9678008.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4798..6261
                     /gene="ttdT"
                     /locus_tag="ACHOQY_001891"
     CDS             4798..6261
                     /gene="ttdT"
                     /locus_tag="ACHOQY_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9678009.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6304..7317)
                     /gene="tsaD"
                     /locus_tag="ACHOQY_001892"
     CDS             complement(6304..7317)
                     /gene="tsaD"
                     /locus_tag="ACHOQY_001892"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9678010.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7555..7770
                     /gene="rpsU"
                     /locus_tag="ACHOQY_001893"
     CDS             7555..7770
                     /gene="rpsU"
                     /locus_tag="ACHOQY_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9678011.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7881..9626
                     /gene="dnaG"
                     /locus_tag="ACHOQY_001894"
     CDS             7881..9626
                     /gene="dnaG"
                     /locus_tag="ACHOQY_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9678012.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            9821..11662
                     /gene="rpoD"
                     /locus_tag="ACHOQY_001895"
     CDS             9821..11662
                     /gene="rpoD"
                     /locus_tag="ACHOQY_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9678013.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11741..12247)
                     /gene="mug"
                     /locus_tag="ACHOQY_001896"
     CDS             complement(11741..12247)
                     /gene="mug"
                     /locus_tag="ACHOQY_001896"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9678014.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12372..12447
                     /locus_tag="ACHOQY_001897"
     tRNA            12372..12447
                     /locus_tag="ACHOQY_001897"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12405..12407,aa:Ile,seq:cat)
     gene            complement(12501..13265)
                     /gene="nfeF"
                     /locus_tag="ACHOQY_001898"
                     /gene_synonym="yqjH"
     CDS             complement(12501..13265)
                     /gene="nfeF"
                     /locus_tag="ACHOQY_001898"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9678015.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13542..14165
                     /gene="nfeR"
                     /locus_tag="ACHOQY_001899"
                     /gene_synonym="yqjI"
     CDS             13542..14165
                     /gene="nfeR"
                     /locus_tag="ACHOQY_001899"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9678016.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14319..15839)
                     /gene="aer"
                     /locus_tag="ACHOQY_001900"
     CDS             complement(14319..15839)
                     /gene="aer"
                     /locus_tag="ACHOQY_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9678017.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16257..17636
                     /gene="ygjG"
                     /locus_tag="ACHOQY_001901"
     CDS             16257..17636
                     /gene="ygjG"
                     /locus_tag="ACHOQY_001901"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9678018.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(17678..18010)
                     /locus_tag="ACHOQY_001902"
     CDS             complement(17678..18010)
                     /locus_tag="ACHOQY_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9678019.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            18229..19212
                     /gene="ebgR"
                     /locus_tag="ACHOQY_001903"
     CDS             18229..19212
                     /gene="ebgR"
                     /locus_tag="ACHOQY_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9678020.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19396..22488
                     /gene="ebgA"
                     /locus_tag="ACHOQY_001904"
     CDS             19396..22488
                     /gene="ebgA"
                     /locus_tag="ACHOQY_001904"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9678021.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22485..22934
                     /locus_tag="ACHOQY_001905"
     CDS             22485..22934
                     /locus_tag="ACHOQY_001905"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9678022.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            22997..24430
                     /locus_tag="ACHOQY_001906"
     CDS             22997..24430
                     /locus_tag="ACHOQY_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9678023.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            24564..25634
                     /gene="ygjJ"
                     /locus_tag="ACHOQY_001907"
     CDS             24564..25634
                     /gene="ygjJ"
                     /locus_tag="ACHOQY_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9678024.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            25651..28002
                     /gene="ygjK"
                     /locus_tag="ACHOQY_001908"
                     /pseudo
     CDS             25651..28002
                     /gene="ygjK"
                     /locus_tag="ACHOQY_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            28328..30346
                     /gene="fadH"
                     /locus_tag="ACHOQY_001909"
     CDS             28328..30346
                     /gene="fadH"
                     /locus_tag="ACHOQY_001909"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9678025.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         7338 a         8199 c         7866 g         6967 t
ORIGIN      
        1 cggtgcgaac aatcaccccc accccggccc tctccctaca gagcgaggga gaaaaccagg
       61 cctgctcccg aaccttttct caatcaaaag aacacgacgt acaccgcagc cgccacaata
      121 aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag gaaggtttta
      181 atcgctatca gcgccaccac aaaagcggtg ataaacccaa cggcaaacat cgggatatcg
      241 ccggttgtca ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc catcatcatc
      301 ggtaccgcca gcaggaacga gaactcggaa gcagcgtaac ggctcacgcc catcagcatc
      361 ccacctgaaa tggtggcccc ggaacgggag aaacccggcc acagcgccag acactggaaa
      421 cagccaatca taaacgcctg acgataggtc atatcatcaa gacccggcgc acgcggctct
      481 ttcggcttca ggcattcggc ggcaatcagc aacaaaccgc caacgaccag tgcatacatc
      541 acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga acaacagccc cagtactacc
      601 gccggaatca tccccagcaa aatgtggatc agcgttaaac gacctttgct ttctccttcg
      661 tgctgcaacg ggcggccaaa gtggatgcca atcaggccaa acagacgccg ccagaacatc
      721 actactaccg ccagaattga tcctaactgg atcacaactt caaaggtttt cgccgtgtcg
      781 ccctcgaacc ccaacaagtg accgacaata atcatatggc ccgtgctgga taccggcaga
      841 aattctgtca atccttcgac cacacccaat attgccgcta tcagcagcga gtgcatatcg
      901 ctcatcaata aacccctaaa ttattaaaat gtaccgcttg tccgaactac tgcgtatgac
      961 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     1021 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     1081 tgatacgcac ccacggcgag ttaaagcgtg ctaacagcag ctccgccacc tcttcagcca
     1141 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     1201 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     1261 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     1321 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     1381 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     1441 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     1501 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     1561 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     1621 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcccgtctgg
     1681 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     1741 cttggacaca tctggcccgt tttcttcgga tttaaaggag gaaaaggcgt tgctaccgct
     1801 tttggtgcca tcgcacccat tggctgggat ctcaccggag taatggcggg aacctggtta
     1861 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     1921 ccgttttatg tatggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
     1981 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
     2041 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
     2101 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
     2161 ttccacgcag acacgtaatt tcatgctgcg atagagcggc tcccggtaaa cagcccagat
     2221 attggcgctt tgtgcatact ccggcaatac ttgcaccagt ttgccactct ccagaaacgg
     2281 caacacatcc cactcggaac gcagcataat ccctttgccc tccagcgccc attgcaacac
     2341 tatctcgccg ctattggagg aaagatgccc gctaaccttc accgattttt tctcctgacc
     2401 gttccccaac tcccatattc cgtgggtcat atcgcgctct ttggtcacca gacagtcatg
     2461 acgacttaat tcctgtaatg attgtggctg cggatatttt tgcagatact caggtgctgc
     2521 gcataatatt cttttatttt tcgttaacag atgcgcaata taataatcag gaatttcgtc
     2581 attaatacga atatccagat caatattatc ctgcactaaa tcaatttgcc gatcgaacaa
     2641 ttcaaaatgc acctgtaact caggataatt gcgcataagt tcggtaatgg ctggcgcaat
     2701 atggctgcgc ccaaaaccaa aactgcaacc aatacgaatc atcccctccg ggcgcgtttt
     2761 gatttgcgtg acgtcatcga ccagtcgctg atattgggtg aggatttcca gcgcatgttc
     2821 gtaacaacgt tggccacttt cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt
     2881 tgttgccagc gtattttcaa ggatttggat ccgcttggtg acaaatgcag gcgtctgccc
     2941 aagcgtcgcc gctgcagcac taaaactccc gctgtggacg atttctacca gtacctgcaa
     3001 gtctttggct aaaggccagc tattcagcat cgttaattat ccgcagttgt gataagcgca
     3061 gtgtatttcg caaaacattg ccattccctg ctctggcgca atattcattc ttattttcgc
     3121 gttgattcac gaactgttaa ttacatattc acggtggcaa aaaatataaa accacatttt
     3181 tagtggtagt ttgtggcggt gaatttttcc agacaaatac aaaaactgga gttgccatga
     3241 tgagcgaaag taataagcaa caggcagtga ataagttgac agagattgtc gctaacttta
     3301 ccgccatgat ttctacccga atgcctgatg acgtggtgga taaactaaaa cagctaaagg
     3361 atgccgaaac gtcgtcgatg gggaaaatta tctaccatac gatgttcgac aacatgcaaa
     3421 aagcgattga cctgaatcgt cctgcctgtc aggacaccgg ggagattatg ttcttcgtta
     3481 aagtcggttc ccgcttccca ctgcttggcg agctgcaaag catactcaaa caagccgtgg
     3541 aagaggcaac cgtcaaagcg ccactacgtc acaatgcggt agaaattttt gacgaagtaa
     3601 acaccggcaa aaataccggt agcggcgtac cgtgggtcac ctgggacatc atccccgaca
     3661 atgacgatgc ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta cctggccgct
     3721 cgaaagtgtt aatgccgtca gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata
     3781 tctccaccct cgccgtaaac gcctgtccac cggtactggt gggcgtgggc atcgccacct
     3841 cggtggaaac cgccgccgtg ctctcgcgaa aagccatttt gcgcccgatt ggcagccgcc
     3901 atcccaatcc aaaagcggca gagctggagc tacgcctgga agaaggactc aaccgtctgg
     3961 gaattggccc acaagggctg actggcaaca gttcagtgat gggcgtgcat atcgaatctg
     4021 ccgcccgcca tccgtcaacc atcggcgttg ctgtttctac aggctgctgg gcgcatcgtc
     4081 gcggcacact gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg
     4141 cgttatgaaa aagatcctga caaccccgat caaagctgaa gatctgcaag atattcgcgt
     4201 cggcgatgtg atctacctga ccggtacgct ggtgacctgc cgtgacgttt gtcaccgccg
     4261 cttgatcgaa ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc
     4321 tggccccatc gtgcgcaaaa acggcgataa atgggagatg gtttccgttg gcccgacaac
     4381 cagtatgcgt atggaaagtt ttgaacgtga atttatcgag cagaccggcg tgaaactggt
     4441 ggttggcaaa ggtggtatgg ggccgctgac cgaagaaggc tgccagaaat tcaaggcgct
     4501 acatgtgatt ttcccggcag gctgcgcggt gctggcggca acccaggtag aagagattga
     4561 agaagtgcac tggacagaac tcgggatgcc ggagtcactg tgggtctgcc gggtcaaaga
     4621 gttcggcccg ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa
     4681 aaagctgttc gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta
     4741 catcaaataa ccctcccgga gaggctcacc cctctccttt ttcgcaggca taacacgatg
     4801 aaaccttcca ctgaatggtg gcgatatctt gcgccgctgg cggtcatcgc cattattgct
     4861 ctacttccgg ttcccgcagg gctggagaac catacctggc tctactttgc cgtttttact
     4921 ggcgtgatcg ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt
     4981 atctccatca tcgccatact ctctccctgg ctgctgttca gccccgaaca gctcgcccag
     5041 ccaggcttta aattcactgc aaaatccctc tcgtgggcgg tttccggttt ttctaattcg
     5101 gttatctggc tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt
     5161 ggacgccgca ttgcgctgtt tctggtgaaa aagatgggac atcgcacgct gtttctcggc
     5221 tatgcggtga tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt
     5281 ggtgcgggga ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca
     5341 aacgacagca gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac
     5401 tgcgtgacca gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg
     5461 atgaaaagcg catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg
     5521 ctcagtattt tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta
     5581 ctgaagtctg gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg
     5641 ctctgttcgc gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt gctgtggatt
     5701 ttcggcggtg attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg
     5761 cttctgcgca ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc
     5821 ttctggctgg cctcgcttat caccctcgct accggactca acaacactgg ttttattagc
     5881 tggtttggca aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg
     5941 gcgttgattg tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc
     6001 tccgccctcg caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg
     6061 gtgttctgcc tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc
     6121 accggaccca gtccgattta ctacggtagt ggttatctgc caacggtgga ttactggcga
     6181 ctgggggcga tttttggact gatattcctc gtgttgctgg tgattaccgg cttgctgtgg
     6241 atgcccgtgg tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcaccggata
     6301 gttttacgca gccggtaact ctgccagtgg ccagcgcgga cgcacgctaa cgccgagatc
     6361 cgccgtcgcg cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc
     6421 agtacaaaat tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttccgc
     6481 cagcttcgcc cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa
     6541 gcccgtctga tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc
     6601 aaaggcgcgg gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt
     6661 cgccgcaaag gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg
     6721 acgcgggaaa acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc
     6781 gccaggataa tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc
     6841 atcgatagac tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt
     6901 atgaccgccg gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat
     6961 cggcgctaac agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa
     7021 cgccagagaa cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc
     7081 ggtataggcc acagcatcaa tgtcttttgc cgttaaacca gactccttta gcgccgcctg
     7141 gatcaacggt acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc
     7201 gtagtcagcg tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc
     7261 gtcgtaaatg gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac
     7321 ttgttttacc tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt
     7381 tgttcgcccc tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa
     7441 aaaggccgcg gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa
     7501 ataagctggc gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg
     7561 gtaattaaag tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc
     7621 tgcgaaaaag caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact
     7681 accgaacgta agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc
     7741 gaaaacgcac gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac
     7801 cgagttgtag ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga
     7861 attgctaaaa atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc
     7921 tggcacgcac tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca
     7981 agaatttcca cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg
     8041 gtgagaaaca gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc
     8101 tgatgaacta cgacaagctc gagttcgttg aaacggtcga agagctggca gcaatgcaca
     8161 atcttgaagt gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc
     8221 aaacgcttta tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac
     8281 ctgttgccac gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg
     8341 ctcgctttgc gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg
     8401 gcaatccaga aaatcgccag tcattgattg atgcggggat gttggtcact aacgatcagg
     8461 gacgcagtta cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc
     8521 gggtgattgg ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc
     8581 cggaaacaga cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg
     8641 ataacgctga acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg
     8701 cgcaatacgg cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca
     8761 tacaactgtt gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag
     8821 gccgcgatgc cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc
     8881 agctacgctt tatgtttttg ccggatggcg aagaccctga cacgctggta cgaaaagaag
     8941 gtaaagaagc gtttgaagcg cggatggagc aggcgatgcc actctccgca tttctgttta
     9001 acagcctgat gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc
     9061 tggcactgcc attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg
     9121 aattaggcaa caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag
     9181 cggcagagag cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac
     9241 ttatagggtt gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc
     9301 tggatgaaaa taagctccct ggacttggct tattcagaga actggtcaac acttgtctct
     9361 cccagccagg tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg
     9421 ccacccttga aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa
     9481 ccttcaccga ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt
     9541 taatcgctcg tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat
     9601 taaacctgga gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa
     9661 gcccccgaca gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc
     9721 gttggcggta aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg
     9781 cccaacacca acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc
     9841 acagctgaaa cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt
     9901 caatgaccat ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat
     9961 gatcaacgac atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct
    10021 ggctgaaaac accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt
    10081 ggaatctgaa atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac
    10141 cgttgaactg ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat
    10201 caaccaggtt caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca
    10261 gtacgatcgt gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga
    10321 cccgaacgca gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca
    10381 ggaagatctg gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga
    10441 tgatgacaac agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca
    10501 gtacgttgta acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga
    10561 agagatcctg aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga
    10621 ctacctggtc aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat
    10681 catgaagctc tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac
    10741 cggcaacgaa accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc
    10801 ggaaaaactg cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat
    10861 tgaagaagaa accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat
    10921 cggtgaagcg aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt
    10981 tatttctatc gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga
    11041 aggcaacatc ggtctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt
    11101 ctccacctac gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc
    11161 gcgcaccatc cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc
    11221 tcgccagatg ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat
    11281 gctgatgccg gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat
    11341 ggaaacgccg atcggtgatg atgaagattc gcatctgggg gatttcatcg aggataccac
    11401 cctcgagctg ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cgcacgacgt
    11461 gctggctggc ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat
    11521 gaacaccgac cacacgctgg aagaagtggg taaacagttc gacgttaccc gcgaacgtat
    11581 ccgtcagatc gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgtt
    11641 gcgtagcttc ctggacgatt aatcggtagg ccggatcagg cgttacgccg cacccggcac
    11701 tagtcccttt gcacaaacgc caccttttcg gtggcgtttt ttatcgccca cgcactacca
    11761 gcgcctggtc cagctcgcga tatgcttcaa ccagtttctc tagtgaaacg cgacttaaac
    11821 cgctgggatt tggcagcacc caaatctgcg tcgaaccaat ggtgagcgtt tgtttccccc
    11881 actgtgcacc gcgctggctg aatccctgtt catatgcttg tttgcccaga atcgccaacg
    11941 cctgcggctg ataatcttca attttttcaa tcagcttacg cccacctgcg tgcagctcct
    12001 gctttgaaac ttcattggct tgcaccgttg gacggtctac cagtttggtg acgccacaac
    12061 gataatccag cagatgctgt gcctcctgcg gcttcaactg acggtcggta aacccggcct
    12121 gatatatcac cttccagaag cgatttgctg gatgagcaaa gggaaaacca gtcccggcgg
    12181 atgaaagccc agggttgata ccgcaaaaca cgacccgtaa ccctggagcc aaaatatcct
    12241 caaccatatt tactcctttc taacatcacc aggaaaagta taacggattg aaaatacatt
    12301 gtttataaaa acagcaggcg cacggtaatg gctggattgc gacacggagt tactttataa
    12361 tccgctacca tggcccctta gctcagtggt tagagcaggc gactcataat cgcttggtcg
    12421 ctggttcaag tccagcaggg gccaccagat atagcaaagg ctgacgagaa atcgtcagcc
    12481 tttttctttt tatatatcag ttactttgcg tgccagtaag ccgctgcacg tacccgctgt
    12541 gggtcatact gttccgcttc aaagcggcgg cttaaattct taacgacttt accttcgccg
    12601 gttatccaga tgaagtaatc atcggcaggg atttgcatct gcgccagacg cgcatctacc
    12661 gcctgctcat catgtgccag ccattcgata ttaaaaccat caaggtgcgc gagataatcc
    12721 tgacaggcgt tatcccgcac gctaaccagc gcactaactt gcggtttaac ggcaagtttg
    12781 ctcaacgttt ccaggcggcg gcgcagtgcg ggcattccgg attcatcgca gacatacagc
    12841 tgatacgcgt aatcttccgg caccaccagc gaaccgcgcg gacctgccac cgtaagttta
    12901 tcgcccggtt gcgcctgcat cgcccagccg ctggcgaccc caccgtcgtg aataaagaaa
    12961 tcgatcgcca gttcatggcg tagttcgtca tacagcggcg tatagtcacg cgacagtggg
    13021 cgtggtcctt ccggccagac gatgccctct tccgttaccg ttggcggcac aaagtgagcg
    13081 tcaggttgag gaaagaagag tttgctgtga tcgtcaaagc cgcgcgatgt aaaaccgtcc
    13141 agcgcctcgc cgccgaggac aatgcgctga aaaccggcgc tgatgcgctc aacgcgtaac
    13201 acagtcagtt cacggaagcg cagatcattg cgaacgcgct gcgggtagcg gggggagtta
    13261 ttcattgtta tcgccttcgt gatagtaatc agatatatct aaatgatttt cgtaaatgat
    13321 aatgattgtt aatcatgata aatgcaagcg atttgtagaa ctgatatatc tgaagtatgt
    13381 gaaggcattc agtgccatag caatgctgcc ggatgcggcg tgaacgtctt atccggcctg
    13441 gatgtgaggt gcttccacgt ttaaaaatca tttttgtact tgcagtaatg ctcacatcag
    13501 atataaatta gatatatcta atcaagcaaa aggaggctga tatgagccat catcacgaag
    13561 ggtgttgtaa acatgaaggc cagccacgcc atgagggctg ctgcaaaggt gagaagtcag
    13621 aacacgagca ctgcggacac ggtcaccagc atgaacacgg tcaatgctgc ggtggtcgcc
    13681 acggtcgcgg cggcggtcgt cggcaacgtt tctttggtca cggtgaattg cgtctggtga
    13741 ttctggatat tctctcgcgc gatgacagcc acggttacga attgattaaa gcgattgaga
    13801 acctaaccca ggggaattac accccaagcc cgggcgtcat ctacccaacg ctggattttc
    13861 tgcaggagca gtcgctgatt accatccgcg aagaggaagg tggtaagaag cagattgcgc
    13921 tgaccgaaca aggcgcgcag tggctggaag aaaaccgcga acaggtggag atgattgaag
    13981 aacgcatcaa agcgcgttgc gttggcgcgg cgctgcgcca gaacccgcaa atgaagcggg
    14041 cgctggataa ttttaaagcg gtgctggatt tacacgtcaa ccagagcgat atcagtgatg
    14101 cacaaataaa aaagatcatt gcggtgatcg accgcgccgc ttttgatatt acgcaactgg
    14161 attaatcgcc gcatccgcca gtggcgcggt gcaattgccg gatgcgacgc ttgacgcgcc
    14221 ttatccggcc tacacccgct acacaccccg caggcctgat aagatgcgcc agcatcgcat
    14281 caggcattgt gctccaaccg ccggatccgg cataccgatt aatgcagcac cgtcaccgcg
    14341 tcttccagtc ggctggcgcg gtgtttcacc atcgccgaca cctgcgcact ctcttccacc
    14401 agctcggcat ttttctgggt gatcaggtta agctcatcca ctgcacgggt caggctggaa
    14461 agcccatcgg cctgttccag cgttgaatgg ctaatctggg cgatcaactg ggtgacgttt
    14521 ttcacctgtg ccacaatatc ttccatcgtc cgtccggcgg cgtgtacctg ctgcgaaccg
    14581 gattgcacct tatcagcact ggcatcaatc agcttgcgaa tatcgttggc agcattagcg
    14641 ctgcggctgg ctaaatgacg cacttcccct gccaccactg caaaaccttt gccctgttcg
    14701 ccggcacgcg ccgcttccac cgccgcattc agggccagaa tattggtctg aaacgcaata
    14761 tcgttaatca gcgaagtaat ggtgccaatg cgctgggtac tgtcggcgat atcgtccatt
    14821 gtcttgatca ccgtggtcat cgcctcccca ccctgcactg ccgcattact ggcggtgata
    14881 gaaagtttat cggcagccga cgccgtggca gagttctgtt tcaccgacgc cgccatttgg
    14941 ttcatggtcg ccaccgtttg ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt
    15001 tcatcggtgc ctttcgccag cgtctcactg ccatttctga cactggacac ctggcttgag
    15061 acatcgttaa ttagccaacg gcacatcagg ccaagctgcc ctaccgcgcg taatgtcagc
    15121 cccagctcat cgctgcgatt caggtgctca acactattac gctctccggt cgccaccttc
    15181 agcgcctgac gggcgacatt ttctatcggg cgcacaatct gccattcaaa acaagcgctt
    15241 gccaacaata ccactaacac acagaggaaa tacgtcacca ccggggcagc aacaaaccaa
    15301 agcatggccg ccagcaagat aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc
    15361 ggtaatgaag gcagtttacc cagccagcct ttacgcacca ccaggccttt atgaatacgc
    15421 ttactggtac gtccggcgtt cagcgctttg tacagcggct ccaccgccgc aatctcttca
    15481 tccgtcgccc gggtacgaat cgacatatag ccactgattt ttccctcgcg caccatcggt
    15541 accgcattgg cccgcaccca ataatggtcg ccatttttgc ggcgattttt cacgatgccg
    15601 ctccagggct cccctttttt cagggtgaac cacatatccg caaacgccgc ttttggcata
    15661 tccgggtgac gcaccatgtt gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc
    15721 acctgcacaa aagtgtcatt agcatgagtg atatagcttt gcagatcggt agtggacatc
    15781 agagtggtat cgtccgccag cggggtattt tgctgggtga catacggatg agaagacatg
    15841 ttcgcgtcct gtgcaggtta tatggttgtt aacttcttgt cagagtttat gtcggccccg
    15901 ctgcggttat ctttaaccga ttaatttgat ttagatcgca atttgcgatt aaagcacaaa
    15961 tctaattctt tgatttaaaa tactttcact ctgttactat acgaaaacgt taattatctt
    16021 gcccaaaaat caggcaatta ttgccctgaa aacgtgcatt tgcgcagcaa tcatcaaatc
    16081 catacccgac aaaaaccgtg caaaataaca acaaatgtta acagatagca ttaaatattg
    16141 cacaaatgat aaccgaattt gtgtttatcc cgattttcgc gatcgcagcc ggagtggcgc
    16201 aatccctgcg atacttaaat cggtatcatg tgatacgcga gcctccggag catattttga
    16261 acaggttacc ttcgagcgca tcggctttag cgtgcagcgc ccacgccctg aatctcattg
    16321 agaagcgaac gctggatcat gaggagatga aagcacttaa ccgagaggtg attgaatact
    16381 tcaaagagca tgtcaatccg gggtttttag agtatcgcaa atctgttacc gccggcgggg
    16441 attacggagc cgtagagtgg caagcgggag gtttaaatac gcttgtcgac acccagggac
    16501 aggagtttat cgactgcctg ggaggttttg gaattttcaa cgtggggcac cgtaatccag
    16561 ttgtggtttc cgccgtacag aatcaacttg cgaaacaacc gctgcacagc caggagctgc
    16621 tcgatccgtt acgggcgatg ttggcgaaaa cccttgctgc gctaacgccc ggtaaactga
    16681 aatacagctt cttctgtaat agcggcaccg agtccgttga agcagcgctg aagctggcga
    16741 aagcttacca gtcaccgcgc ggcaagttta cttttattgc caccagcggc gcgttccacg
    16801 gtaaatcact tggcgcgctg tcggccacgg cgaaatcgac cttccgcaaa ccgtttatgc
    16861 cactgctgcc gggcttccgt catgtgccgt ttggcaatat cgaagccatg cgcacggctc
    16921 ttaacgagtg caaaaaaacc ggtgatgatg tggctgcggt gatcctcgaa ccgattcagg
    16981 gtgaaggtgg cgtaattctg ccgccgccgg gctatctcac cgccgtacgt aagctatgcg
    17041 atgagttcgg cgcactgatg atcctcgatg aagtacaaac gggcatgggg cgcacgggca
    17101 agatgttcgc ctgcgagcat gagaacgtac agccggatat cctctgcctt gccaaagcgc
    17161 tcggcggcgg cgtgatgccg attggcgcga ccatcgccac tgaagaggtg ttttcagttc
    17221 tgttcgacaa cccattcctg cataccacca cctttggcgg caacccgctg gcctgtgcgg
    17281 cggcgctggc gaccatcaat gtgttgctgg agcagaactt accggctcag gctgagcaaa
    17341 aaggcgatat gttgctggac ggtttccgtc aactggcgcg ggaatatccc gatctggtac
    17401 aggaagcgcg tggtaaaggg atgttgatgg caattgagtt tgttgataac gaaatcggct
    17461 ataactttgc cagcgagatg ttccgccagc gcgtactggt ggccggaacg ctcaataacg
    17521 ccaaaacgat ccgcattgaa ccgccactga cactgaccat tgaacagtgt gaactggtga
    17581 tcaaagcggc gcgtaaggcg ctggcggcca tgcgagtaag tgtcgaagaa gcgtaatatc
    17641 aaatcggatg gcgatacgac gtcgccatcc gatcttttta tacgacgcgt acgcccgcag
    17701 gcatcatccg ctccggcgtt aacaacacgc tttcactgcc gtcatcggtt tccgcacaca
    17761 acagcatgca ttccgacgtt tcaccacgca ttttcgcttt ttgcagattg cataagacca
    17821 ccaccgtttt ccccatcagc tcttcttcgc tgtagtacgg caccaggctg gtcacggttt
    17881 gcagtgtttt ttcccccaca tcaacctgta cgatgtacag cttgtcggcg ttttcatggc
    17941 gtttcacttc cacaatcttt ccgacgcgca tttccagacg tgcaaaatca gcgtaagcca
    18001 cggtttccat tgtttcctcc cttcgggtaa aattttacta aactatagca aaagtttttc
    18061 tcaatcctgt aggctaaaaa tggagaatgc aggcgtgatc acattcgtga gccgctgtgt
    18121 taccgttaca gcgtcaaaga aacgcgcttt atttactgaa aacaggtgac ccgataagca
    18181 cttcctctac aatgggggcg cacatcaggg aaagtaaaaa aggtaaacat ggcaacacta
    18241 aaagacatcg caatcgaagc tggcgtatcc ctggcgacag tatccagggt cttaaatgac
    18301 gatccgacat tgaatgtgaa agaagagacg aaacatcgca ttctcgagat cgccgaaaag
    18361 ctggagtaca agaccagtag tgcccgtaaa ctccagacag gtgcagtcaa ccaacaccat
    18421 attctggcta tctacagcta ccagcaggag ctggagatca acgatcctta ctatctggcg
    18481 atccgccacg gcattgaaac ccagtgcgaa aagctgggca tcgagctcac caactgttat
    18541 gaacacagcg gcttaccaga cattaaaaac gtcaccggta ttttaattgt cggcaaaccc
    18601 acgcccgccc tgcgcgccgc tgccagcgcg ttgaccgaca atatctgttt tatcgacttt
    18661 cacgaacccg gcagcggtta cgatgcggtg gatatcgatc tggcacgcat cagtaaagaa
    18721 atcatcgact tctatatcaa ccagggcgtt aatcgtattg gttttattgg cggtgaagat
    18781 gagcctggca aggcggatat tcgtgaggtc gcctttgcgg aatatggccg actgaaacaa
    18841 gtggtacgcg aagaggatat ctggcgcggc ggtttttcca gttcgtcggg ttatgaactg
    18901 gcaaaacaaa tgctggcgcg ggaagactat ccgaaggcac tgtttgttgc ttccgattcc
    18961 attgctatcg gcgtactgcg ggcaattcat gaacgaggcc tgaacatccc acaggatatt
    19021 tcgcttatca gcgttaacga tatccccacc gcgcgattta cctttccgcc gctctccacc
    19081 gtgcgcatcc attccgagat gatgggaagt cagggcgtta acctggtgta tgaaaaagcc
    19141 cgcgatggtc gcgcgctgcc gctgttagtc ttcgttccca gcaaattaaa actgcgcggc
    19201 acgacccgtt aaatcccctt acacactgtc cggcaatcgt ttttgccgga cagtgctgcc
    19261 gtttattttc gtgatccagt taaagtaaat gcatttacct gctacttttt agtaaaaatt
    19321 ttactaaact ccccagcaat tacacaaact accatcacca tgaatggttc cgatttctct
    19381 ctaccgggag gccctatgaa tcgctgggaa aacattcagc tcacccacga aaaccgactt
    19441 gcgccgcgtg cgtacttttt ttcatatgat tctgttgcgc aagcgcgtac ctttgcccgc
    19501 gaaaccagca gcctgtttct gcccttaagc ggtcagtgga atttccactt ttttgaccat
    19561 ccgctgcaag taccagaagc cttcacctct gagttaatgg ctgactgggg gcatattacc
    19621 gtccccgcca tgtggcaaat ggaaggtcac ggcaaactgc aatataccga cgaaggtttt
    19681 ccgttcccca tcgatgtgcc gtttgtcccc agcgataacc caaccggtgc ctatcaacgt
    19741 attttcaccc tcagcgacgg ctggcagggt aaacagacgc tgattaaatt tgacggcgtc
    19801 gaaacctatt ttgaagtcta tgttaacggt cagtatgtgg gtttcagcaa gggcagtcgc
    19861 ctgaccgcag agtttgacat cagcgcgatg gttaaaaccg gcgacaacct gttgtgtgtg
    19921 cgcgtgatgc agtgggcgga ctctacctac gtggaagacc aggatatgtg gtggtcagcg
    19981 gggatcttcc gcgatgttta tctggtcgga aaacacctaa cgcatattaa cgatttcact
    20041 gtgcgtaccg actttgacga agcctattgc gatgccacgc tttcctgcga agtggtgctg
    20101 gaaaatctcg ccgcctcccc tgtcgtcacg acgctggaat ataccttgtt tgatggcgaa
    20161 cgcgtggtgc acagcagcgc cattgatcat ttggcaattg aaaaactgac cagcgccagc
    20221 tttgctttta ctgtcgaaca gccgcagcaa tggtcagcag aatcccctta tctttaccat
    20281 ctggtcatga cgctgaaaga cgccaacggc aacgttctgg aagtggtgcc acaacgcgtt
    20341 ggcttccgtg atatcaaagt gcgcgacggt ctgttctgga tcaataaccg ttatgtgatg
    20401 ctgcacggcg tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt tggaatggat
    20461 cgcgtcgaga aagatctcca gttgatgaag cagcacaata tcaactccgt gcgtaccgct
    20521 cactacccga acgatccgcg tttttacgaa ctgtgtgata tctacggctt gtttgtgatg
    20581 gcggaaaccg acgtcgaatc gcacggcttt gctaatgtcg gcgatatcag ccgtattacc
    20641 gacgatccgc agtgggaaaa agtctacgtc gagcgcattg ttcgccatat tcacgcgcag
    20701 aaaaaccatc cgtcgatcat catctggtcg ctgggcaatg aatccggcta tggctgtaac
    20761 atccgcgcga tgtaccacgc agcgaaggcg ctggatgaca cgcgactggt gcattacgaa
    20821 gaagatcgcg atgctgaagt ggtcgatatt atttccacca tgtacacccg cgtgccgctg
    20881 atgaatgagt ttggtgaata cccgcatccg aagccgcgca tcatctgtga atatgctcat
    20941 gcgatgggga acggaccagg cgggctgacg gagtaccaga acgtcttcta taagcacgat
    21001 tgtattcagg gacattatgt ttgggagtgg tgcgaccacg ggatccaggc gcaggatgac
    21061 aacggcaacg tctggtataa attcggcggc gactacggcg actatcccaa caactataac
    21121 ttctgtcttg atggtttgat ctattccgat cagacgccgg gaccgggcct gaaagagtac
    21181 aaacaggtta tcgcgccggt aaaaatccac gcgctggatc tgactcgcgg cgagctgaaa
    21241 gtcgaaaata aactgtggtt taccacgctt gatgactaca ccctgcacgc agaggtgcgc
    21301 gtcgaaggtg aaacgctcgc gacgcagcag attaaactgc gcgacgttgc gccgaacagc
    21361 gaagccccct tgcagatcac gctgccgcag ctggacgccc gcgaagcgtt cctcaacatt
    21421 acggtgacca aagattcccg cacccgctac agcgaagccg ggcattctat cgccacttat
    21481 cagttcccgc tgaaggaaaa caccgcgcaa ccagtgcctt tcgcaccaaa taatgcgcgt
    21541 ccgctgacgc tggaagacga tcgtttgagc tgcaccgttc gcggctacaa cttcgcgatc
    21601 accttctcaa aaatgagtgg caaaccgaca tcctggcagg tgaatggcga gtcgctgctg
    21661 acccgcgagc caaagatcaa cttcttcaag ccaatgatcg acaaccacaa gcaggagaac
    21721 gaagggctgt ggcaaccgaa tcatttgcag atcatgcagg aacatctgcg cgactttgcc
    21781 gtagaacaga gcgatggtga agtgttgatc atcagccgca cagttattgc cccgccggtg
    21841 tttgacttcg ggatgcgctg cacctacatc tggcgcatcg ctgccgatgg ccaggttaac
    21901 gtggcgcttt ccggcgagcg ttacggcgac tatccgcaca tcattccgtg catcggtttc
    21961 accatgggga ttaacggcga atacgatcag gtggcgtatt acggtcgtgg accgggcgaa
    22021 aactacgccg acagccagca ggctaacatc atcgatatct ggcgcagcac cgtcgatgcc
    22081 atgttcgaga actatccctt cccgcagaac aacggcaacc gtcagcatgt ccgctggacg
    22141 gcactgacta accgccacgg caacggtctg ctggtggttc cgcagcgccc aattaacttc
    22201 agcgcctggc actataccca ggaaaacatc cacgctgccc agcactgtaa cgagctgcag
    22261 cgcagtgatg acatcaccct gaacctcgac caccagctac ttggcctcgg ctccaactcc
    22321 tggggcagcg aggtgctgga ctcctggcgc gtctggttcc gtgacttcag ctacggcttt
    22381 acgttgctgc cggtttctgg cggagaagct accgcgcaaa gcctggcgtc gtatgagttc
    22441 ggcgcagggt tcttttccac gaatttgcac agcgagaata agcaatgagg atcatcgata
    22501 acttagaaca gttccgccag atttacgcct ctggcaagaa gtggcaacgc tgcgttgaag
    22561 cgattgaaaa tatcgacaac attcagcctg gcgtcgccca ctccatcggt gactcattga
    22621 cttaccgcgt ggagacagac tccgcgaccg atgcgctatt taccgggcat cgacgctatt
    22681 ttgaagtgca ttactacctg caagggcagc aaaaaattga atatgcaccg aaagagacat
    22741 tacaggtagt ggaatattat cgtgatgaaa ctgaccgtga atatttaaaa ggctgcggag
    22801 aaaccgttga ggtccacgaa gggcaaatcg ttatttgcga tatccatgaa gcgtatcggt
    22861 ttatctgcaa taacgcggtc aaaaaagtgg ttctcaaagt caccatcgaa gatggttatt
    22921 tccataacaa ataacaacta cggcggcaaa aggagtttgc cgccaccgct accctacaca
    22981 ttttcggaga tgtgttatgt ctgataccaa acgtaataca atcggcaaat tcggcttgct
    23041 ctcgctgact tttgccgccg tttacagctt taacaacgtt atcaataata atattgagct
    23101 tggactggcc tcggcaccga tgtttttcct cgcgacgatt ttttatttta ttcccttctg
    23161 tctgatcatc gcagaatttg tttcgttaaa taaaaactca gaagccggtg tctacgcgtg
    23221 ggtaaaaagt tcgctgggcg gacgttgggc atttattact gcctatacct actggttcgt
    23281 aaacctgttc tttttcacct cactgttgcc gcgcgttatt gcttatgctt cgtatgcctt
    23341 cctcggatac gaatatatta tgacgccggt tgccaccacc attatcagta tggtgctgtt
    23401 cgccttctcc acctgggttt ccaccaacgg ggcgaaaatg ttggggccaa ttacctccgt
    23461 cacttcaacg ctgatgctgc tgttaacgct ctcctacatt ttactggcag gtacggcgct
    23521 ggttggcggc gtacagcctg ctgaccccat caccgttgac gcgatgatcc cgaacttcaa
    23581 ctgggcgttc ctcggcgtta ccacctggat ctttatggcc gcaggtggcg cggagtccgt
    23641 cgctgtgtac gttaacgacg tcaaaggcgg ttcgaaatcg ttcgttaaag tgatcatcct
    23701 cgccgggatt tttatcggcg tactgtattc cgtctcctcg gtgctgatta acgtcttcgt
    23761 cagcagcaaa gagttgaaat ttaccgtcgg atcggtgcag gtattccacg gcatggcggc
    23821 gtattttggt ctaccggaag cgttgatgaa tcgctttgtc ggtctggtgt cctttaccgc
    23881 gatgttcggt tccctgctga tgtggaccgc aacgccggtg aaaattttct tctccgaaat
    23941 cccggaaggc atctttggta agaaaaccgt cgaactgaac gaaaacggcg ttccggcgcg
    24001 cgcagcgtgg atccagttcc tgatcgtcat cccgctgatg attatcccga tgctcggttc
    24061 caataccgtg caggatctga tgaatactat tattaatatg accgccgcag cgtccatgct
    24121 tccgccgtta ttcatcatgc tggcttacct gaatttacgc gccaaattag atcacctgcc
    24181 acgcgatttc cgtatgggct cccgccgcac cggtattatc gttgtttcaa tgctgattgc
    24241 gatatttgcc gtagggtttg tcgcttcgac attcccgact ggcgcgaata ttctgaccat
    24301 cattttttat aacgtcggcg gtattgttat cttccttggc tttgcgtggt ggaaatacag
    24361 taaatatata aagggattaa cggctgaaga gcgccatatt gaagcgacgc cagccagcaa
    24421 tgttgattaa gcagaataat aaaaaatgag caggcatttt ccctcttcct ggatgaggag
    24481 agggaaaata gtttctgcct ttatattttt ataattacaa cgataaaagg ctgtattttt
    24541 tctttagctc atggattaac acaatgaaat taatcactgc accatgcaga gcattacttg
    24601 ctctgccgtt ttgctacgcc ttttctgcgg caggagaaga agcacgtccg gcagaacatg
    24661 acgacacaaa aacacctgca attacctcga catcttctcc ttcatttcgt ttttacggcg
    24721 aattaggggt tggtggatat atggatttag agggtgagaa taaacataaa tacagcgacg
    24781 gtacctatat tgaaggtggc ctggagatga agtacggctc ctggttcggc ctgatttacg
    24841 gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc ctgggtgcca gaccatagct
    24901 ggggtggttt cgagggcgga attaaccgtt tctatggcgg ttatcgtacc aatgatggca
    24961 ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga tgacctgcaa tggtggggcg
    25021 acttcacccc cgatctgggc tacgtcattc ccaatacccg cgacattatg accgcgctga
    25081 aggtgcagaa cttaagcggc aactttcgtt atagcgtcac cgccacacct gccggacatc
    25141 atgacgaaag caaagcctgg ctacattttg gcaagtacga tcgctatgac gacaaataca
    25201 cctatccggc aatgatgaac ggttacatcc agtatgacct tgccgaaggc atcacctgga
    25261 tgaacggtct ggaaatcacc gacggcacag gacagctcta tctcacgggc ctgctaactc
    25321 ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc cgacgggctg gacgtaccgg
    25381 gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc gttaaagggc gtttatctct
    25441 ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc tgacgatgaa accacctctt
    25501 tcatgcagtt tggtatctgg tacgaatacg gcggcggacg tttcgccacg gcttttgata
    25561 gccgcttcta catgaaaaat gcctctcacg atcccagcga ccaaatcttc ctgatgcaat
    25621 atttctactg gtaataagga ctgtaatttc atgaaaataa aaactatttt aacgccagta
    25681 acctgcgctc tgctgataag tttttccgcc catgccgcta acgccgacaa ttataaaaac
    25741 gtgattaacc gtactggcgc gccgcagtac atgaaggatt acgattacga cgatcaccag
    25801 cgttttaatc cgtttttcga tctcggagcc tggcatggtc atctgttgcc agacggccct
    25861 aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg aagagtacat caactttatg
    25921 gccagcaatt tcgaccgcct gaccgtctgg caggacggca agaaagtcga ctttacgctg
    25981 gaggcataca gtattcccgg tgcgctggtg caaaaactga cagcaaaaga tgtgcaagtc
    26041 gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac tggaaaccaa aatcaccagc
    26101 aataaaccgc tggatctggt gtgggacggc gaactgctgg aaaaactgga agcgaaagaa
    26161 gggaaaccgc tttccgataa aaccattgct ggcgaatacc ctgactacca gcgcaaaatc
    26221 agcgccaccc gtgatggcct gaaagtcacc tttggcaaag tgcgcgccac ctgggatctg
    26281 ctgacctccg gcgaatcgga atatcaggtg cataaatccc tgccggtgca gactgaaatc
    26341 aacggcaatc gctttaccag taaggcacat atcaacggtt cgacgacgct ctataccacc
    26401 tattcccatc tgctgacctc tcaggaagtt agcaaagagc aaatgcagat ccgcgatatt
    26461 ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac gctgggaaga gtatctgaag
    26521 aaagggttaa ccaatccgga tgcgacgccg gaacagacgc gcgtcgcggt gaaagcgatc
    26581 gaaacgctca acggtaactg gcgctcaccg ggcggtgcgg tgaaatttaa caccgttaca
    26641 ccgtcggtga ccgggcgctg gttctccggc aatcagacct ggccgtggga tacctggaag
    26701 caggcgtttg cgatggcgca tttcaatccg gacatcgcca aagagaatat ccgcgcggtc
    26761 ttctcctggc agatccagcc tggcgatagc gtgcgtccgc aggatgtggg ctttgtcccc
    26821 gacctgatag cgtggaacct tagccccgag cgtggtggcg atggcggcaa ctggaacgaa
    26881 cgtaatacca aacccagcct tgccgcctgg tcggtgatgg aagtgtacaa cgtcacccag
    26941 gataaagcct ggttggcaga gatgtacccg aaactggtgg cctatcacga ctggtggtta
    27001 cgtaaccgcg atcacaacgg caacggcgtg ccgtaatatg gcgcgacccg cgacaaagcc
    27061 cacaacactg agagcggcga gatgctgttt acggtgaaga aaggcaacaa agaagagacg
    27121 cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag gccagtacga cagtctggaa
    27181 attccggcac aggtcgctgc gtcgtgggaa tcggggcgtg atgatgccgc cgtctttggt
    27241 tttatcgaca aagaacagtt ggataaatat gtcgctaatg gcggcaaacg tagcgactgg
    27301 acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt tgctgggcta ctcgctattg
    27361 caggagtcgg tggatcaggc cagctatatg tacagcgata accattatct ggcggagatg
    27421 gcaaccatcc tcggtaagcc ggaagaggcc aaacgctatc gccagttggc acagcagctc
    27481 gcagactaca taaacacctg tatgttcgac ccggctaccc agttctacta tgacgtgcgt
    27541 attgaagata aaccgctggc gaacggctgc gcgggcaaac cgattgtcga gcgcggtaaa
    27601 gggccggaag gctggtcgcc gctgtttaac ggtgcggcaa cgcaggccaa cgccgacgcg
    27661 gtggtgaagg tgatgctcga tcctaaagag ttcaacacct ttgtcccgct gggaacggcg
    27721 gcgttaacca atccggcttt tggcgctgat atctactggc gcgggcgcgt atgggtggat
    27781 cagttctggt ttggtctgaa agggatggag cgttacggtt atcgtgatga tgccctgaaa
    27841 ctggcggata cgttcttccg tcacgccaaa ggattaaccg ccgatggccc aattcaggag
    27901 aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa atttctcctg gagtgccgcg
    27961 catttgtata tgttgtataa cgattttttc cgtaagcaat aatttgcaac tgctggcgga
    28021 tgcggcgtaa acgccttatc cgccctacat gtgcaatcct cctggccgga taaaacgcga
    28081 caagcgtcgc atccggcatc tgcaccgtgc cactggcgga tgcggcgtga acgccttatc
    28141 cgccctacat gtgcaatccc ccaggccgga taaaacgcgg caagcgtcgc atccggcgtt
    28201 accaccgggc gtattctttt tgaatcccat cacaaacccc gcactcccct tttccctttt
    28261 ctccggcgac ggctaaatta gaactcatcc gaccacataa caattatttt acatactgga
    28321 caattttatg agctacccgt tgctgttcgc cccgctggat ttaggtttta ccacgttaaa
    28381 aaaccgcgtg ttgatgggct caatgcacac cgggctggag gaatacccgg acggtgccga
    28441 gcggctggca gcgttttatg ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg
    28501 cggcattgca ccagatttaa caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc
    28561 cagccagatc ccacaccatc gcaccattac cgaagcggta catcaggaag gcggcaaaat
    28621 agccctgcaa attttgcata ccgggcgcta cagctaccaa ccgcatctgg tcgcttcctc
    28681 cgcattgcag gcccccatca accgtttcgt tcctcacgaa ctcacccatg aagagatcct
    28741 gcaactgatc gacgatttcg cccactgcgc gcaactggca cgggaggcag gatacgacgg
    28801 tgtagaggta atggggtccg aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa
    28861 tcagcgtagt gaccagtggg gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt
    28921 agtgcgtgcg gtgcgcgaac gcgtcggcaa cgacttcatt attatctacc gactgtcgat
    28981 gctcgacctg gtcgaagacg gcgggacttt tgccgaaacg gtagagctgg cgcaggccat
    29041 tgaagcggcg ggcgcgacca ttatcaacac cggcattggc tggcatgaag cacgtattcc
    29101 gaccattgcc acgcccgtgc cgcgcggcgc atttacttgg gtcacgcgca aactgaaagg
    29161 ccacgtttcg ctgccgctgg tgaccaccaa ccggattaac gatccgcagg ttgccgacga
    29221 cattctctcg cgcggcgatg ccgatatggt atcgatggca cgaccgtttc ttgctgatgc
    29281 ggaactgctg tcaaaagcgc aatcggggcg agccgatgag atcaacacct gcattggctg
    29341 caatcaggcc tgcctcgatc agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc
    29401 tcgcgcctgc cacgaaacca aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc
    29461 ggtggtcggt gcgggacctg ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca
    29521 tcatgtaaca ttgtttgatg cccacagcga gatcggcggg cagtttaata tcgccaaaca
    29581 gatccccggc aaagaggagt tttacgaaac gctgcgctat taccaccgga tgatcgaagt
    29641 gacgggcgtg acgctgaaac tcaatcacac cgtgacggcg gatcagttac aggcgttcga
    29701 tgaaacgatc ctcgccagtg ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca
    29761 tccgaaggta ttgagttatc tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt
    29821 tgccatcatc ggttgtggcg ggattggttt tgatacggcg atgtatttaa gtcagccggg
    29881 cgaatccacc agccagaata tcgccaggtt ctgtaatgag tgggggatcg acagcagcct
    29941 acaacaggct ggtggcttaa gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat
    30001 tgtgatgctc cagcgcaaag ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg
    30061 gatccatcgc accaccctgc tctcgcgggg tgtgaaaatg atcccaggcg taagttatca
    30121 gaagattgac gatgacgggc tgcatgtggt gatcaacggc gaaacgcagg tattagcagt
    30181 ggacaatgtg gtgatctgcg cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat
    30241 tgatagcggg aaaactgtgc atttaattgg cggctgcgat gtggctatgg agctggacgc
    30301 acgacgggca attgcccagg gaacacggct ggcgctggag atttaaatcg ttatttgaag
    30361 cgccggatgc
//
LOCUS       DAZKGS010000044        30235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKGS010000044 DAZKGS010000000
VERSION     DAZKGS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHOQY_001910"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHOQY_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9678026.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHOQY_001911"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHOQY_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9678027.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHOQY_001912"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHOQY_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9678028.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHOQY_001913"
     CDS             complement(1087..1581)
                     /locus_tag="ACHOQY_001913"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9678029.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHOQY_001914"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHOQY_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9678030.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHOQY_001915"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHOQY_001915"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9678031.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHOQY_001916"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHOQY_001916"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9678032.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHOQY_001917"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHOQY_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9678033.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHOQY_001918"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHOQY_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9678034.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHOQY_001919"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHOQY_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9678035.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHOQY_001920"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHOQY_001920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9678036.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHOQY_001921"
     CDS             complement(9275..9769)
                     /locus_tag="ACHOQY_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9678037.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHOQY_001922"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHOQY_001922"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9678038.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHOQY_001923"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHOQY_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9678039.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHOQY_001924"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHOQY_001924"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9678040.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHOQY_001925"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHOQY_001925"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9678041.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHOQY_001926"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHOQY_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9678042.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHOQY_001927"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHOQY_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9678043.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHOQY_001928"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHOQY_001928"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9678044.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHOQY_001929"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHOQY_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9678045.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHOQY_001930"
     CDS             17948..18874
                     /locus_tag="ACHOQY_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP9678046.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHOQY_001931"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHOQY_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9678047.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHOQY_001932"
     CDS             complement(19225..21444)
                     /locus_tag="ACHOQY_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9678048.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHOQY_001933"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHOQY_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9678049.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHOQY_001934"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHOQY_001934"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9678050.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHOQY_001935"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHOQY_001935"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9678051.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHOQY_001936"
     CDS             complement(26816..27298)
                     /locus_tag="ACHOQY_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9678052.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHOQY_001937"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHOQY_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9678053.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHOQY_001938"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHOQY_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9678054.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHOQY_001939"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHOQY_001939"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9678055.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..30233)
                     /locus_tag="ACHOQY_001940"
     CDS             complement(29946..30233)
                     /locus_tag="ACHOQY_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP9678056.1"
                     /translation="MMLTYWLIDAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMI
                     AAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7467 a         8079 c         7633 g         7056 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcagatc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgt
//
LOCUS       DAZKGS010000045        29615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKGS010000045 DAZKGS010000000
VERSION     DAZKGS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHOQY_001941"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHOQY_001941"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9678057.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHOQY_001942"
     CDS             complement(804..1352)
                     /locus_tag="ACHOQY_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9678058.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHOQY_001943"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHOQY_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9678059.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHOQY_001944"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHOQY_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9678060.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTNCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHOQY_001945"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHOQY_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9678061.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHOQY_001946"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHOQY_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9678062.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHOQY_001947"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHOQY_001947"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9678063.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHOQY_001948"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHOQY_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9678064.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHOQY_001949"
     CDS             complement(11782..12675)
                     /locus_tag="ACHOQY_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9678065.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHOQY_001950"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHOQY_001950"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9678066.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHOQY_001951"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHOQY_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9678067.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKRELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHOQY_001952"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHOQY_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9678068.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHOQY_001953"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHOQY_001953"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9678069.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHOQY_001954"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHOQY_001954"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9678070.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHOQY_001955"
     CDS             complement(17843..20107)
                     /locus_tag="ACHOQY_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9678071.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHOQY_001956"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHOQY_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9678072.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHOQY_001957"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHOQY_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9678073.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHOQY_001958"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHOQY_001958"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9678074.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHOQY_001959"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHOQY_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9678075.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHOQY_001960"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHOQY_001960"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9678076.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHOQY_001961"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHOQY_001961"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9678077.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHOQY_001962"
     CDS             complement(26972..27664)
                     /locus_tag="ACHOQY_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9678078.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHOQY_001963"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHOQY_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9678079.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7191 a         8057 c         7190 g         7177 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgcg gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaattggt caagccttcc aggtgttcag
     5941 ttatctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacggcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcgccgca ctgttcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagc tcgtcaatgt caaaattctt gccctgacgt ttgcagaaat
     6481 ctgccagcag acgctcagct tcttgctgtt cgcgcagacg ctgttccagt tcgcttaagc
     6541 gcatccgcaa cggctgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 tagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ttcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtc ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggagatggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgtga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 cgtttttcct ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc gcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccaccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgctgt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 tgctacgctg atggctatct tcaacaaaga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggc gtttatgctt gccgtattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttt
//
LOCUS       DAZKGS010000046        29526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKGS010000046 DAZKGS010000000
VERSION     DAZKGS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..998
                     /gene="ybhD"
                     /locus_tag="ACHOQY_001964"
     CDS             45..998
                     /gene="ybhD"
                     /locus_tag="ACHOQY_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9678080.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1039..2034)
                     /gene="pgl"
                     /locus_tag="ACHOQY_001965"
     CDS             complement(1039..2034)
                     /gene="pgl"
                     /locus_tag="ACHOQY_001965"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9678081.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2189..3007
                     /gene="ybhA"
                     /locus_tag="ACHOQY_001966"
     CDS             2189..3007
                     /gene="ybhA"
                     /locus_tag="ACHOQY_001966"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9678082.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3008..4066)
                     /gene="modC"
                     /locus_tag="ACHOQY_001967"
     CDS             complement(3008..4066)
                     /gene="modC"
                     /locus_tag="ACHOQY_001967"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9678083.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4069..4758)
                     /gene="modB"
                     /locus_tag="ACHOQY_001968"
     CDS             complement(4069..4758)
                     /gene="modB"
                     /locus_tag="ACHOQY_001968"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9678084.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4758..5531)
                     /gene="modA"
                     /locus_tag="ACHOQY_001969"
     CDS             complement(4758..5531)
                     /gene="modA"
                     /locus_tag="ACHOQY_001969"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9678085.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5697..5846)
                     /gene="acrZ"
                     /locus_tag="ACHOQY_001970"
     CDS             complement(5697..5846)
                     /gene="acrZ"
                     /locus_tag="ACHOQY_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9678086.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5975..6763
                     /gene="modE"
                     /locus_tag="ACHOQY_001971"
     CDS             5975..6763
                     /gene="modE"
                     /locus_tag="ACHOQY_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9678087.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6831..8303
                     /gene="modF"
                     /locus_tag="ACHOQY_001972"
     CDS             6831..8303
                     /gene="modF"
                     /locus_tag="ACHOQY_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9678088.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8564..9580
                     /gene="galE"
                     /locus_tag="ACHOQY_001973"
     CDS             8564..9580
                     /gene="galE"
                     /locus_tag="ACHOQY_001973"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9678089.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9590..10636
                     /gene="galT"
                     /locus_tag="ACHOQY_001974"
     CDS             9590..10636
                     /gene="galT"
                     /locus_tag="ACHOQY_001974"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9678090.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10640..11788
                     /gene="galK"
                     /locus_tag="ACHOQY_001975"
     CDS             10640..11788
                     /gene="galK"
                     /locus_tag="ACHOQY_001975"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9678091.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11782..12822
                     /gene="galM"
                     /locus_tag="ACHOQY_001976"
     CDS             11782..12822
                     /gene="galM"
                     /locus_tag="ACHOQY_001976"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9678092.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13025..13777
                     /gene="gpmA"
                     /locus_tag="ACHOQY_001977"
     CDS             13025..13777
                     /gene="gpmA"
                     /locus_tag="ACHOQY_001977"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9678093.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13936..14988)
                     /gene="aroG"
                     /locus_tag="ACHOQY_001978"
     CDS             complement(13936..14988)
                     /gene="aroG"
                     /locus_tag="ACHOQY_001978"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9678094.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPQAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15304..15684
                     /gene="ybgS"
                     /locus_tag="ACHOQY_001979"
     CDS             15304..15684
                     /gene="ybgS"
                     /locus_tag="ACHOQY_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9678095.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15798..16739
                     /gene="zitB"
                     /locus_tag="ACHOQY_001980"
     CDS             15798..16739
                     /gene="zitB"
                     /locus_tag="ACHOQY_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9678096.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16736..17455)
                     /gene="pnuC"
                     /locus_tag="ACHOQY_001981"
     CDS             complement(16736..17455)
                     /gene="pnuC"
                     /locus_tag="ACHOQY_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9678097.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17493..18536)
                     /gene="nadA"
                     /locus_tag="ACHOQY_001982"
     CDS             complement(17493..18536)
                     /gene="nadA"
                     /locus_tag="ACHOQY_001982"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9678098.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18785..18909)
                     /locus_tag="ACHOQY_001983"
     ncRNA           complement(18785..18909)
                     /ncRNA_class="other"
                     /locus_tag="ACHOQY_001983"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18941..19016)
                     /locus_tag="ACHOQY_001984"
     tRNA            complement(18941..19016)
                     /locus_tag="ACHOQY_001984"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18981..18983),aa:Lys,seq:ttt)
     gene            complement(19050..19125)
                     /locus_tag="ACHOQY_001985"
     tRNA            complement(19050..19125)
                     /locus_tag="ACHOQY_001985"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19090..19092),aa:Lys,seq:ttt)
     gene            complement(19272..19347)
                     /locus_tag="ACHOQY_001986"
     tRNA            complement(19272..19347)
                     /locus_tag="ACHOQY_001986"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19312..19314),aa:Lys,seq:ttt)
     gene            complement(19351..19426)
                     /locus_tag="ACHOQY_001987"
     tRNA            complement(19351..19426)
                     /locus_tag="ACHOQY_001987"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19391..19393),aa:Val,seq:tac)
     gene            complement(19576..19651)
                     /locus_tag="ACHOQY_001988"
     tRNA            complement(19576..19651)
                     /locus_tag="ACHOQY_001988"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19616..19618),aa:Lys,seq:ttt)
     gene            complement(19654..19729)
                     /locus_tag="ACHOQY_001989"
     tRNA            complement(19654..19729)
                     /locus_tag="ACHOQY_001989"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19694..19696),aa:Val,seq:tac)
     gene            complement(19865..19940)
                     /locus_tag="ACHOQY_001990"
     tRNA            complement(19865..19940)
                     /locus_tag="ACHOQY_001990"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19905..19907),aa:Lys,seq:ttt)
     gene            complement(20105..20896)
                     /gene="cpoB"
                     /locus_tag="ACHOQY_001991"
     CDS             complement(20105..20896)
                     /gene="cpoB"
                     /locus_tag="ACHOQY_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9678099.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20906..21427)
                     /gene="pal"
                     /locus_tag="ACHOQY_001992"
     CDS             complement(20906..21427)
                     /gene="pal"
                     /locus_tag="ACHOQY_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9678100.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21462..22754)
                     /gene="tolB"
                     /locus_tag="ACHOQY_001993"
     CDS             complement(21462..22754)
                     /gene="tolB"
                     /locus_tag="ACHOQY_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9678101.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22887..24152)
                     /gene="tolA"
                     /locus_tag="ACHOQY_001994"
     CDS             complement(22887..24152)
                     /gene="tolA"
                     /locus_tag="ACHOQY_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9678102.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24217..24645)
                     /gene="tolR"
                     /locus_tag="ACHOQY_001995"
     CDS             complement(24217..24645)
                     /gene="tolR"
                     /locus_tag="ACHOQY_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9678103.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24649..25341)
                     /gene="tolQ"
                     /locus_tag="ACHOQY_001996"
     CDS             complement(24649..25341)
                     /gene="tolQ"
                     /locus_tag="ACHOQY_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9678104.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25338..25742)
                     /gene="ybgC"
                     /locus_tag="ACHOQY_001997"
     CDS             complement(25338..25742)
                     /gene="ybgC"
                     /locus_tag="ACHOQY_001997"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9678105.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25892..26185)
                     /gene="ybgE"
                     /locus_tag="ACHOQY_001998"
     CDS             complement(25892..26185)
                     /gene="ybgE"
                     /locus_tag="ACHOQY_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9678106.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26185..26298)
                     /gene="cydX"
                     /locus_tag="ACHOQY_001999"
     CDS             complement(26185..26298)
                     /gene="cydX"
                     /locus_tag="ACHOQY_001999"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9678107.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26313..27452)
                     /gene="cydB"
                     /locus_tag="ACHOQY_002000"
     CDS             complement(26313..27452)
                     /gene="cydB"
                     /locus_tag="ACHOQY_002000"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9678108.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27468..29036)
                     /gene="cydA"
                     /locus_tag="ACHOQY_002001"
     CDS             complement(27468..29036)
                     /gene="cydA"
                     /locus_tag="ACHOQY_002001"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9678109.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7353 a         7799 c         7264 g         7110 t
ORIGIN      
        1 cgaccagcca cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat
       61 caagtatgaa ggcatttgtc atactggcag agtccagttc atttaacaat gctgctaaat
      121 tactcaatat tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac
      181 atattcaact atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt
      241 tgctcccgga ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg
      301 atatgaatgc ttatcatcgt ggtatggtga cgttagcgtg tattccgacc gcagtatttt
      361 attttttacc gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga
      421 ttctggaaca aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg
      481 gcataaacat gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc
      541 cattcgtgct ggcctgtcgg cgtgaccatc cattggccaa aaagcagctt gtagaatggc
      601 aggagttagt gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga
      661 tagaacaaca gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc
      721 tttcgacgtc attaggactg gtagaggcgg gactggggat ttcggcgctt cctggtctgg
      781 caatgcccca tgcgccctat tcttcgatta ttggtattcc actggtggaa cccgttatac
      841 gtcggacatt ggggattatt cgccgcaaag atgccgtgct ttctccggca gcagaacgct
      901 tttttgcctt gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg
      961 tcgaacgcca gagacatgcg ttacaggaga taggttaata caccggcgca ggagagactg
     1021 cgccggttaa atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac
     1081 cgcataacgg cctttttcat gcagtagtcc ctgctcgcca acaatttcgt ataccgagat
     1141 gtggtgagat ttttgcccgg cggcaatcag atacttgccg ctgtgatcaa cattgaagcc
     1201 gcgcggctgg gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga
     1261 aacgctgaaa acggtaatca ggctggcggt acggtcgcag gcgtataaat ggcgaccatc
     1321 cggggtgata tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc
     1381 cagcgtctgg acacattcga tattaccgtg cggatctttc agttcccaga catccactga
     1441 gctgtttaac tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg
     1501 cgggccggcc ccttcaacgg tggtcacttc cgcagggtcc tgcgccacga gatgaccatc
     1561 atcgctgacc gtaaacaggc aaatgcgatc ctgctttaat gccggaaccc acagcgtacg
     1621 gttgtccggt gagatattgg cggaatggca accgtccagc ccctcgacca catcgacgac
     1681 gcccactggc aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc
     1741 tacaaagaca aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcgc
     1801 agactctgcg gcaaaggtca gtgcgccatc gtccggggcg atactatacg ccaggacgcg
     1861 aaactcaggg cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg
     1921 cacctgcccc ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt
     1981 ccagacgtga atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaatgc
     2041 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
     2101 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
     2161 aattcttaac cttaatctgg aatacgccat gaccacacgc gtgattgctc tcgacttaga
     2221 cggcacctta ttgaccccga aaaagaccct gcttccttca tcgatagaag ccctggcccg
     2281 cgctcgcgaa gcaggctatc aattaatcat cgtcacaggt cgccatcacg tcgctattca
     2341 tcctttttat caggcgctgg cgctggatac acctgctatt tgctgtaatg gcacctattt
     2401 gtatgattat catgcaaaaa ccgtgctgga agcggaccca atgcccgtta ataaagccct
     2461 gcaactcatt gagatgctga atgaacacca cattcacggt ctgatgtatg tggatgatgc
     2521 aatggtctat gagcacccga ccgggcatgt cattcgcacg tctaactggg cgcaaaccct
     2581 gccgccggaa cagcgtccga ctttcacaca agtcgcttct ctggctgaaa cggcgcaaca
     2641 agttaacgcc gtatggaagt tcgccctgac gcacgatgac ctgccgcaat tgcagcattt
     2701 tggtaagcat gtcgaacatg aactgggact ggagtgtgaa tggtcctggc acgatcaggt
     2761 tgatattgca cgcggcggca acagcaaagg taaacgtttg acgaaatggg ttgaggcgca
     2821 aggctggtcg atggaaaacg tcgtggcgtt cggcgataac tttaatgata tcagtatgct
     2881 ggaggccgct ggtacaggcg tggcgatggg caacgccgat gacgcggtaa aagcgcgcgc
     2941 caacattgtg attggtgata acaccaccga cagcattgcc cagttcattt atagccacct
     3001 gatttaatca ggcggttatc gacacacttt taatttgcgc gtacagccac aggccaggtt
     3061 tgatcaccag ttcatccctg gcccacgggc tgatacgcgc ccacagcgtt ttacctccga
     3121 cttccagttc cacttccacc tggccgttgt cgtcataact attaacaact tttgcccgca
     3181 atacgttacg aatgctggtt tgctgcggcg gttgtaaaac cagagaaaca tcggaagcct
     3241 gaatgcggat acgtagcgca gcttgcagcg gttcgtccag cttattgacc cacaaatgct
     3301 gatcgcccag cgccagcgca gtcatcgcgt aatgcggatg atgctcaagt accgtcactt
     3361 tcagaatgct actttgttgc tctttcggca gccacggatt catcacgcta ctaccccaga
     3421 cttcttccag tgcgccaaag gctttcacct gaccgttttc cagtaccatc actctgtctg
     3481 ccagatggag gatctcatcc agcgaatggc tgacatacaa catcgggatg ttgatttccc
     3541 gcgtcagacg ttgcagataa ggcaacagtt cgcgtttacg cggaatatcc agtgacgcca
     3601 gcggttcatc cagcaacagc aattctggtg ccgtcagcaa agcccgccca atcgctacgc
     3661 gctgtttttc gcctccggac aggctgcctg gtaaacggtc aagcaacggt tcaatgccta
     3721 aaagcgccac cagcttatcg aactgatcga ccatgctttt cgccatgccg tagcgcagat
     3781 tgccacgcac tttgtaatgc ggaaacagcc gcgcatcctg aaaaacatag ccaacgcgac
     3841 gcttttctgg cgtcaggcag ataccttttt cggcatcatt tagtacccga ccattgagga
     3901 caatcctccc tttttgcggg cgcgtcagtc cactgatggc gttaatcagc gaagtttttc
     3961 cggcaccgga gacgccaaag atagcagtga tgccattggc gggcagcgtt tcattaatgg
     4021 tcaggcaatg gttgcccaag gtctgggaaa aattcagttc cagcatgatt agcgccccgc
     4081 ccgttcacgg ctgattctgg ccagccattc tgaaatcaac agggagatca tcgccagcgc
     4141 aatagaaata atgcacagtc tcgccgcgcc actttccccg ccgggggtct ggatcagggt
     4201 atacatggca gaaggaatgg ttcgcgtttc accaggaatg ttcgacacaa aggtgatggt
     4261 tgcaccaaac tcaccgagag aacgagcaaa agccagtacc gtaccaacaa taattcccgg
     4321 taaggtcagc ggtaacgtga tagtaaagaa aacgcgccag cgcccggccc ccagtgttct
     4381 tgcggcctgt tcgagtttga catcaacccc ttccagcgcc agacgaattg cccgcaccat
     4441 cagcggaaac gacatgacgg cggcagcgag aaccgcgccg cgccagctaa aggcgaaggt
     4501 aataccaaac cagtcataca gacgttcacc gataaatccg cgccgtccca tcgaaactaa
     4561 taataagtaa ccgacgacca cgggcggtaa caccagcggt agatgcagta cgctgtcgag
     4621 cagagctttg cccggaaacg tgcaacgcac cagtaaccag gcaaaaaaga tcccaaacgg
     4681 caggctaaac agcacagcca gggaagaaac tttcaggctt aataaaactg cctgccattc
     4741 tggatcggtc agtatcatta cttggttgta aatccgtaac gtttaaagat ttccgcagcc
     4801 tgcggccctt tcaaataatc ataaaatgct ttcactgtcg cattgttatg ctcttccaca
     4861 acagcaaccg gatattccac ttttttatgt gaatcttccg ggaaggtggc aaccactttt
     4921 acccctttgc tggcaactgc gtcagaaccg taaacaatgc ccaacggcgc ttcattgcgt
     4981 tcgaccaacg ccagcgcgcc acgaacatct tccgccggag ccagtttcgg ggagagcgta
     5041 tcccatgcgc ccagtttttg cagtgcttct ttcgcataaa tgccagcagg gacatgttcc
     5101 ggatcgccaa ccgcgaggcg accgccattc agcagcgaag tccagttggt tttgctgtcg
     5161 atggtgaaat ctttctgctc gctggctttc ggtgctacaa cgaccaggct attgccgagc
     5221 agtgtctggc gcgaagccgt atcgatcgct tttttatcaa ccgcataatc catccatttc
     5281 tgatcggcag agataaacag atccgcaggc gctcccgctt caatctgacg ggccagagta
     5341 gatgacgaag cgaaagaaga aaccacatcc acgccttttt cttttttata ctgcgtagca
     5401 atgtcctgca ttgcgttagt cagtgatgcg gcggcgaaca ccgtgatttt cccttcatct
     5461 gccagtgtat tgccagcaac agcgaaagag agtgccgccc cggcaaacaa gttcaaccat
     5521 ttacgagcca ttggtaaccc cttaatgtaa cgttatgtag gcgacaatat aacggctagc
     5581 tcagaatttt cccagcagtt attgctaacc tttgtgaggt agataagaaa aaatatcggc
     5641 tggaaaagca ggaagttgag aaaaagaaat gcccgactaa gcgggcattc agggaatcaa
     5701 tgattttgtc cgggctggtc ttttttacca acaccagaaa agatgttgaa tacttcacca
     5761 agaccgtaaa tcagacccag gatgatggcc atcacgacag gtaccatgat tacggcgaat
     5821 accagacttt ttaataactc taacatggtc aactccagat atagtcatga gactattcta
     5881 accgctaagc acagaaaagc actccccttt tgtgcggtca gctttgcgtg gcgttcgttt
     5941 tccgtcacaa taagactttt gccaggacat tgttatgcag gccgaaatcc ttctcaccct
     6001 taagctccaa caaaaattat tcgccgaccc gcgccggatt tcgctactaa aacacattgc
     6061 gctttccggt tccattagcc agggagcgaa agatgccggt atcagctata aaagcgcctg
     6121 ggatgccatt aacgagatga atcagttaag tgagcatatt ctggtcgagc gcgcaacagg
     6181 cggtaaaggt ggcggcggcg cagtactgac ccgctatggt cagcgactga ttcagctcta
     6241 tgacttactg gcgcaaatcc agcaaaaagc ctttgatgtg ttaagtgacg atgacgccct
     6301 gccgctgaac agcctgctgg ccgcgatctc acgtttttca ctgcaaacca gcgcccgtaa
     6361 ccagtggttc ggcaccatca ccgcccgcga tcatgatgac gttcaacagc atgttgatgt
     6421 cttactggct gacggaaaaa cacgcctgaa agtcgcaatt accgcacaaa gcggcgcgcg
     6481 tctggggctg gatgaaggca aagaagtgtt gatattgcta aaagcgccgt gggtaggtat
     6541 tactcaggac gaggcggtcg cacaaaacgc cgacaaccaa ttaccgggta ttattagtca
     6601 tattgagcgc ggcgcagagc agtgcgaagt attaatggcg ctacccgacg ggcaaacact
     6661 gtgcgccaca gtgccggtaa atgaagcgac ttctcttcag caagggcaga atgtcacggc
     6721 ctactttaat gccgacagcg tgattatcgc cacgctgtgc taagcgtgtt gacaatttgt
     6781 tatgaaacac gtatccctgt cagtaatcgc tgcacaaaat gggatataaa atgtcatcgt
     6841 tgcaaatttt gcaaggcacg tttcgtctta gcgacacaaa aacgctgcaa ttgcctcagc
     6901 taacgttaaa cgcgggtgat agttgggcgt ttgtcggttc gaatggaagc gggaaatcgg
     6961 ccctggcccg cgcgctggcg ggggaacttc cgcttttgaa aggtgaacgg caaagccagt
     7021 tttcccacat cactcgtctc tccttcgagc aattgcaaaa actcgtcagc gacgaatggc
     7081 agcggaataa caccgatatg ctcggccctg gcgaagatga caccggacgc actacggctg
     7141 agatcattca ggatgaagta aaggatgcac cgcgttgcat gcaactggcg cagcagttcg
     7201 gtattaccgc cctcctcgac cgacgcttta aatacctttc cactggcgag acgcgaaaaa
     7261 ccctgctgtg tcaggcgctg atgtcggagc ctgacttgtt gattcttgat gagccgttcg
     7321 atggcctgga tgttgcctca cgtcagcagc tggctgagcg actcgcctcg ttacatcagt
     7381 ccggtattac tctggtactg gtgctcaatc gcttcgatga gatcccggag tttgtccagt
     7441 ttgctggcgt gctggcggat tgcacgttag cggaaactgg cgctaaagag gaactgctcc
     7501 aacaagcact cgtcgcgcaa ctggcgcata gtgaacagct tgaaggtgtg caactgccgg
     7561 agccggatga accttcagca cgtcacgcct tacccgccaa cgaaccgcgc attgtgctga
     7621 acaatggcgt ggtttcttat aacgatcgcc ccattcttaa taaccttagc tggcaggtga
     7681 atccaggtga acactggcaa attgtcgggc caaatggtgc aggaaaatcg acgttattaa
     7741 gcctggttac tggcgatcat ccgcaaggtt acagcaacga tttgacgctt ttcggacgac
     7801 gtcgcggcag cggcgaaacc atctgggata tcaaaaagca tatcggttac gtcagcagta
     7861 gtttgcatct ggattaccgg gtcagcacta ccgtgcgtaa tgtgattctt tctggctatt
     7921 ttgattcgat tggcatttat caggccgttt cggatcgcca gcaaaaactg gtgcagcagt
     7981 ggctggatat tctcggcatt gataaacgta cggctgacgc tccgttccat agtctttcct
     8041 ggggacagca gcgtctggcg ctgatcgtcc gcgcactggt gaaacatccg acgttgctta
     8101 ttctcgatga accactacag gggcttgatc cgctcaatcg ccagcttatc cgccgttttg
     8161 ttgatgtgct gattagcgaa ggtgaaacgc aattgttgtt tgtttcgcac cacgctgaag
     8221 atgcgcctgc ctgtattacc catcgccttg agttcgtgcc ggacggtgga ctctatcgct
     8281 atgtgctgac aaaaatatat tgagtcggta gtgctgacct tgccggaggc ggcctaagcg
     8341 ccctctccgg ccaacggttc gacgcatgca ggcatgaaac cgcttctttt ttcagataaa
     8401 aagcgcaatc attcataaac cctctgtttt ataatcactt aatcgcgcat aaaaaacggc
     8461 taaattcttg tgtaaacgat tccactaatt tattccatgt cacacttttc gcatctttgt
     8521 tatgctatgg ttatttcata ccataagcct aatggagcga attatgagag ttctggttac
     8581 cggtggtagc ggttacattg gaagtcatac ctgtgtgcaa ttactgcaaa acggtcatga
     8641 tgtcatcatt cttgataacc tctgtaacag taagcgcagc gtactgcctg ttatcgagcg
     8701 tttaggcggc aaacatccaa cgtttgttga aggcgatatt cgtaacgaag cgttgatgac
     8761 cgagatactg cacgatcacg ctatcgacac cgtgatccac ttcgccgggc tgaaagccgt
     8821 cggcgaatcg gtacaaaaac cgctggaata ttacgacaac aatgtcaacg gcactctgcg
     8881 cctgattagc gccatgcgcg ccgctaacgt caaaaacttt atttttagct cctccgccac
     8941 cgtttatggc gatcagccca aaattccata cgttgaaagc ttcccgaccg gcacaccaca
     9001 aagcccttac ggcaaaagca agctgatggt ggaacagatc ctcaccgatc tgcaaaaagc
     9061 ccagccggac tggagcattg ccctgctgcg ctacttcaac ccggttggcg cgcatccgtc
     9121 gggcgatatg ggcgaagatc cgcaaggcat tccgaataac ctgatgccat acatcgccca
     9181 ggttgctgta ggccgtcgcg actcgctggc gatttttggt aacgattatc cgaccgaaga
     9241 tggtactggc gtacgcgatt acatccacgt aatggatctg gcggacggtc acgtcgtggc
     9301 gatggaaaaa ctggcgaaca agccaggcgt acacatctac aacctcggcg ctggcgtagg
     9361 caacagcgtg ctggacgtgg ttaatgcctt cagcaaagcc tgcggcaaac cggttaatta
     9421 tcattttgca ccgcgtcgcg agggcgacct tccggcctac tgggcggacg ccagcaaagc
     9481 cgaccgtgaa ctgaactggc gcgtaacgcg cacactcgat gaaatggcgc aggacacctg
     9541 gcactggcag tcacgccatc cacagggata tcccgattaa ggaacgacca tgacgcaatt
     9601 taatcccgtt gatcatccac atcgccgcta caacccgctc accgggcaat ggattctggt
     9661 ttcaccgcac cgcgctaagc gcccctggca gggggcgcag gaaacgccag ccaaacaggt
     9721 gttacctgcg cacgatccag attgcttcct ctgcgcaggt aatgtgcggg tgacaggcga
     9781 taaaaacccc gattacacca ggacttacgt tttcactaat gactttgcgg ccttgatgtc
     9841 tgacacgcca gatgcgccag aaagcaacga tcctttgatg cgttgccaga gcgcgcgcgg
     9901 taccagccgg gtgatctgct tttcaccgga tcacagtaaa acgttgccag aactgagcgt
     9961 tgcggcattg acggaaatcg tcaaaacctg gcaggagcaa accgcagagc tgggaaaaac
    10021 gtacccgtgg gtgcaggtct ttgaaaacaa aggtgcggcg atgggctgct ctaacccgca
    10081 tccgcacgga cagatttggg caaatagctt cctgcctaac gaagctgagc gcgaagaccg
    10141 cctgcaaaaa gaatattttg ccgaacagaa atcaccaatg ctggtggatt atgttcagcg
    10201 cgagctggca gacggtagcc gtaccgttgt cgaaaccgaa cactggttag ccgttgtgcc
    10261 ttactgggct gcctggccgt tcgaaacgct actgctgccc aaagcccacg ttttgcggat
    10321 caccgatttg accgacgccc agcgcagcga tttggcgctg gcgttgaaaa agctgaccag
    10381 tcgttatgac aacctcttcc agtgctcctt cccctactct atgggctggc acggcgcacc
    10441 atttaatggc gaagagaatc aacactggca gctgcacgcg cacttttatc cgcctctgct
    10501 gcgctccgcc accgtacgta aatttatggt tggttatgaa atgctggcag agacccagcg
    10561 agacctgacc gcagaacagg cagcagagcg tttgcgcgca gtcagcgata tccattttcg
    10621 cgaatccgga gtgtaagaaa tgagtctgaa agaaaaaaca caatctctgt ttgccaacgc
    10681 atttggctac cctgccactc acaccattca ggcgcctggc cgcgtgaatt tgattggtga
    10741 acacaccgac tacaacgacg gtttcgttct gccctgcgcg attgattatc aaaccgtgat
    10801 cagctgtgcg ccacgcgatg accgtaaagt tcgcgtaatg gcagccgatt atgaaaatca
    10861 gctcgacgag ttttccctca atgcgcccat tgtcgcgcat gaaaactatc aatgggcgaa
    10921 ctacgttcgt ggcgtggtga aacatctgca actgcgtaac aacagcttcg gcggtgtgga
    10981 catggtgatc agcggcaatg tgccgcaggg tgccgggtta agttcttccg cttcactgga
    11041 agtcgcggtc ggaaccgtat tgcagcagct ttattatctg ccgctggacg gcgcacaaat
    11101 cgcgcttaac ggtcaggaag cagaaaacca gtttgttggc tgtaactgcg ggatcatgga
    11161 tcagctaatt tccgcactcg gcaagaaaga tcatgccttg ctgattgact gtcgctcact
    11221 ggggaccaaa gcagtttcca tgccgaaagg tgtggctgtc gtcatcatca acagtaactt
    11281 caaacgtacc ctggttggca gcgaatacaa cacccgtcgt gaacagtgcg aaaccggtgc
    11341 gcgtttcttc cagcagccag ccctgcgcga tgtcaccatt gaagagttca acgctgttgc
    11401 acatgagctg gacccaatcg tggcgaaacg cgtgcggcat atcctgactg aaaacgcccg
    11461 caccgttgaa gctgccagcg cgctggagca gggcgacctg aaacgtatgg gcgagttgat
    11521 ggcggagtct catgcctcta tgcgcgatga tttcgaaatc accgtgccgc aaattgacac
    11581 tctggtagaa atcgtcaaag ctgtgattgg cgacaaaggt ggcgtacgca tgaccggcgg
    11641 cggatttggc ggctgtatcg tcgcgttgat cccggaagag ctggtgcctg ccgtacagca
    11701 agctgtcgct gaacaatatg aagcaaaaac aggtattaaa gagacttttt acgtttgtaa
    11761 accatcacaa ggagcaggac agtgctgaac gaaactcccg cactggcacc cgatggtcag
    11821 ccgtaccgac tgttaacttt gcgtaacaac gcagggatgg tagtcacgct gatggactgg
    11881 ggtgcgactt tactttcagc ccgtattccg ctttccgatg gcagcgtccg cgaggcgctg
    11941 ctcggctgtg ccagcccgga atgctatcag gatcaggccg cgtttctggg ggcctctatt
    12001 ggtcgttatg ccaaccgcat cgccaatagc cgttatacct ttgacggtga aaccgtgacc
    12061 ctttcgccaa gtcagggcgt taaccagctg cacggcggcc cggaagggtt cgacaaacgt
    12121 cgctggcaga ttgtgaacca gaacgatcgt caggtgctgt ttgccctgag ttcagatgat
    12181 ggcgatcagg gcttcccggg taatctcggc gcaacggtgc aatatcgtct gaccgacgat
    12241 aaccgtatct ccattactta tcgcgccaca gttgataaac cttgcccggt gaatatgact
    12301 aatcacgtct atttcaatct tgacggcgag cagtctgacg tgcgcaatca caagttgcag
    12361 attctggcgg acgaatatct tccggttgat gaaggcggca ttccgcacga cggcttgaaa
    12421 tctgtcgccg gaacgtcttt tgatttccgc agcgccaaaa tcatcgccag tgagtttctt
    12481 gccgacgacg atcagcgcaa agtgaaaggt tacgatcacg cattcttgtt acaggccaaa
    12541 ggcgatggca agaaagtggc ggcgcatgtc tggtcagcag atgaaaaatt gcagctgaag
    12601 gtctacacca ccgctccggc tcttcaattc tactccggca acttcctcgg cggcacaccg
    12661 tcgcggggaa ccgaacctta cgccgactgg caagggctgg cgttggaaag cgagtttctg
    12721 ccggacagcc cgaaccaccc tgaatggccg caaccggact gcttcctgcg tcctggcgaa
    12781 gagtattcca gcctgacgga atatcagttt attgctgagt aatgttaagc cctcgtcatg
    12841 agggctttat cttcatattg ttcaaatcac cagcaaacac cgacatattt gcaactcaat
    12901 attcacaaca accttacact gcgccactat tttcgctatg gttatgcgta agcattgctg
    12961 ttgcttcgtc acggcaatat aatgagaatt attatcatta aaagatgatt tgaggagtaa
    13021 gtatatggct gtaactaagc tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga
    13081 aaaccgtttc accggttggt acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa
    13141 agcagcaggt aagctgctga aagaggaagg ttacagcttt gactttgctt acacttctgt
    13201 gctgaaacgc gctatccata ccctgtggaa tgtgctggac gaactggatc aggcatggct
    13261 gcccgttgag aaatcctgga aactgaacga acgtcactac ggtgcgttgc agggtctgaa
    13321 caaagcagaa accgctgaaa agtatggcga cgagcaggtg aaacagtggc gtcgtggttt
    13381 tgcagtcact ccgccggaac tgactaaaga tgatgagcgt tatccgggtc acgatccacg
    13441 ttacgcgaag ctgagcgaga aagaactgcc gctgacggaa agcctggcgc tgaccattga
    13501 ccgcgtgatc ccttactgga atgaaactat tctaccgcgt atgaagagcg gtgagcgcgt
    13561 gatcatcgct gcacacggta actctttacg tgcgctggtg aaatatcttg ataacatgag
    13621 tgaagaagag attcttgagc tgaatatccc gaccggcgtg ccgctggtgt atgagttcga
    13681 cgagaatttc aaaccgctga aacgctatta tctgggtaat gctgacgaga tcgcagcgaa
    13741 agcagcggcg gttgctaacc agggtaaagc gaagtaaacg tcattcgttt aaaaatgaga
    13801 aagccgactg caagtgagcc ggcttttttg ttgctaacaa tggagcacat tgcctgatgc
    13861 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    13921 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag
    13981 agcatcggta tcttcccagc cgatgcaggc atcggtgatg ctcttaccgt aggcctgcgg
    14041 ctccccgctc tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    14101 aatggctgtt tcgccaccgg caatctgctg gcaaacgtca gcacacacat ccatctgctt
    14161 tttgaattgt ttggacgagt tagcatggct gaaatcgatc atcacctgtg ctggcaggcc
    14221 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    14281 accgccgcgc agaatgatat ggcaatcacc gttaccgctg gtattcacaa tcgccgaatg
    14341 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    14401 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    14461 cgccagttcg cggtgcacct gcgattcggt ggtacgtgca ccaattgcgc cccagctcat
    14521 caggtcagcg agatattgtg gggtgatcat atcgagaaac tcacccgccg ctggcagacc
    14581 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    14641 gctgttatcc atatgcggat cgttaatcag ccctttccag cccaccgtgg tacgcggctt
    14701 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    14761 caagcgagtg gcatactctt ttgctgcgac aggatcatga attgagcatg ggccaatcac
    14821 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    14881 aaccgtattc gcggcatttt cagtagcggg gaatttttcc agcaatgcga caggaggaag
    14941 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    15001 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    15061 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    15121 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    15181 atttcaagat tctctgctgc gtttcataac ctggctgaaa tcttaaacca atgccttata
    15241 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    15301 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    15361 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    15421 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    15481 aataacgggg taaataccgg ttctggtggc acaatgctgc attcggatgg ttcttcaatg
    15541 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcgc
    15601 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    15661 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    15721 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    15781 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    15841 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    15901 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    15961 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    16021 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    16081 ccatagccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    16141 cgccgcgtcc ggtcgagggc ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    16201 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    16261 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    16321 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    16381 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    16441 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    16501 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    16561 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    16621 acctgatgga tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc
    16681 atgggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    16741 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    16801 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    16861 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    16921 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    16981 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    17041 ctgccaggcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    17101 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    17161 actccgcctc gttctgactg gtttgtcgcg accacgcata ccaaccgtaa atattcgcgg
    17221 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    17281 caaacaaggt gacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    17341 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    17401 cgcctgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    17461 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    17521 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tttcatcaac atgaacctcg
    17581 tgattgcttc cttcctgttc taatgcctct gcgatggact gaaggccatt catggccatc
    17641 cacggacaat gcgcgcagct gcggcaggtt gcaccctcac ctgcggttgg tgcttccagt
    17701 aactctttat ctggcactgc ctgctgcatt ttgtagaaaa tgccccgatc ggttgccacg
    17761 ataagccgct gatgtggcaa tgttttcgca gcagcgatca gttgactggt ggaaccgacc
    17821 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    17881 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatacag
    17941 gcaccctgcc agcatagaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    18001 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    18061 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    18121 tagacgacga cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg
    18181 cagccgagat ccagtgaaca ttcagcctga agtgtcggca tcagaattgt tttttccgga
    18241 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    18301 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    18361 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    18421 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    18481 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    18541 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaatattc gctgttatcg
    18601 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    18661 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    18721 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    18781 acattggtgg gtcgtgcagg atgactcgct tcgctcgccc ttcgggccgt cgccgcgagc
    18841 ggctccgttg tttcgctgcg ctcaactcaa acctgctgca ggttcgaatc tttaagtatc
    18901 gcagatagca aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg tgcaggattc
    18961 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgaccccttg
    19021 cgggattatg ctgctttaat cattcaggat ggtgggtcgt gcaggattcg aacctgcgac
    19081 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccatacg ggtgttacct
    19141 gaaagatttc tactcggggc caatctttaa gattggtgtg tcgtgcagga tgactcgctt
    19201 cgcttgccct acgggccgtt gctggcgcaa cgttatcctt cacgcttaac atctgagttt
    19261 gatgttaaaa ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa
    19321 ctgctctacc aactgagcta acgacccgag tggtgggtga tgacgggatc gaaccgccga
    19381 ccccctcctt gtaagggagg tgctctccca gctgagctaa tcacccgata ctgcgctgga
    19441 tactacaaac tacttccacc gaacccaccg taattagtgg tgggtcgtgc aggatgactc
    19501 gcttcgctcg ccctacgggc cgttgctgac gcaacgttat cctacacgct taacatctgt
    19561 cttcgatgct aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    19621 tcaactgctc taccaactga gctaacgacc cggtggtggg tgatgacggg atcgaaccgc
    19681 cgaccccctc cttgtaaggg agatgctctc ccagctgagc taatcaccca atactacgct
    19741 ggatactaca actaactatg ttagtggtgg gtcgtgcagg atgactcgac ttcgtctcgc
    19801 ccttcgggcc gttgctgatg caacgttatc cttcacgttt tactaccgct tccaccttag
    19861 cgattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    19921 accaactgag ctaacgaccc actttttcgt tgctttcggt ttgtttgata tcccgtggca
    19981 acggcggcat atattactga tttcagactt gagcgcaaca aaaatttcga tgcagatcac
    20041 tcaactgctt atgattcgca cgacacgacc agaaataatg cgacttctgg tcgtgtgtta
    20101 tgcattacat cgcgttcaga cgtttttgtg cctgtttagc gccatcagta ccagggtatt
    20161 tactgataac ctgctggtat acggctttcg cttttgcggt gtcacctttg tcctgcatga
    20221 tgacgccgac tttaaacatc gcatcagcag cctttggtga cttcggatag tttttcacta
    20281 ccgaagcaaa atagtacgcc gcatcatctt ttttaccctt gttgtagttt aactgaccca
    20341 gccaataatt ggcgtttggc aggtaagttg aatcagggta atttttgatg aaattctgaa
    20401 atgccaccat tgcgtcatcc tggcgggatt tatcctgcac cagcgcaata gctgcattgt
    20461 aatccgtgtt tgcatcgccg cttttcaccg gcgcgccagc attcgcagta ccagcatcag
    20521 ctgtcggcgt cgttgatgcc gccgcaccgc tttgatcgct gctggttgat tgcgccgctg
    20581 caccaccgct gctgaggctg tcgatctgca acaggatctg cttttgccgc tccacgacct
    20641 gattcagttg atactgattt tcctgaatct gaccacgcag ggaatcaata tcggattgat
    20701 tatcagaaag ttgttgctgg agttgggtta aaagctggct gtgagcatta gaaatacgct
    20761 caagttgagt gacgcggtct tcgaccgagc ctgagccgac actactgatt ggtgcctgag
    20821 caaaagcggc ccagggggcc gctataccaa ccagtaacga cagactcaat agttgatgtc
    20881 tgaagttact gctcatgcaa ttctcttagt aaaccagtac cgcacgacgg tttttggagt
    20941 atgccgcttc gtcatgaccc agtactgcag gtttttcttt accgtaagaa acgatggaga
    21001 tctggtctgc agaaacgcct ttaccctgca ggtacatctt aacggcgttc gcacgacgtt
    21061 cacccaggga gatgttgtat tccggagtac cacgttcgtc cgcgtgacct tctacggtga
    21121 ctttgtaaga cgggttgcta cgcaggaagt ttgcatgtgc atccagcatt tgagcgaagt
    21181 cagaacggat atcgtacttg tccagatcga agtaaacgat gttgttctgc tgcagctgtt
    21241 gcatttgcag acgagcctgc tcttcggaag acatgttgcc gttgccgccg ttcgcatcca
    21301 taccagtgcc ggcacccagc atgccttcgc tgccgtcatt gctggcgttc ttgttggaag
    21361 aacatgccgc aattgccata acaggcagag caatcatcag ccctttcagc actttgttca
    21421 gttgcatttc aataattcct ttactattca attaattatt atcacagata cggcgaccag
    21481 gcagggaatt tgacctgtcc atcagttgcc ggaagacgcg ctttgaaacg cccatctgta
    21541 gaaaccaaat tcagcacgga tcccatcccc tgagaagagc tgtagattac catagtgccg
    21601 ttaggtgcca gacttggcgt ttcatccagg aacgtggacg acagaacttg tacgcctccc
    21661 gttgccagat cttgtttggc aatgtgctgc tgcccaccat tggagctgac cattaccata
    21721 aatttaccgt cgctgctgac atccgcatcc tggttctgcg aaccttccca ggtaatacgt
    21781 tgtggcgcac cgccgttgat attcacttta taaacctgcg gacgaccggc ctggtcagaa
    21841 gtaaatgcca ggttctggct atccgggaac caggtcggtt cggtattgtt actgcgacca
    21901 tcagtcacct ggcggatctg accagaagcc aaatccatta cgtacaggtt cagactaccg
    21961 gttttcgaca aggcgaatgc cagtttgctg ccgtctggcg agaatgcagg tgcaccgttg
    22021 tgacgcggga atgaagccac ctgacgtaca gcgccatttg ccagcgtctg aataaccagc
    22081 gcggaacgac cgctttcgaa ggtcacataa gccagtttag aaccgtctgg tgaccacgcc
    22141 ggagacatca gcggctgcgg tgaacggtga acgacaaact ggttgtaacc gtcatagtca
    22201 gatacgcgca gttcatacgg gaactgaccg ccgttggtct gaacaacgta ggcaatacga
    22261 gtacggaacg cacctttaat gccggtcagt ttttcaaaca cttcatcact ggcggtatga
    22321 ccagcataac gcagccactg cttgttcact ttgtacgagt tctgagcaag tacagtaccc
    22381 ggtgcgccgc cagtgtcaac aagttgataa gcaacattgt aagaaccatc cggattcgga
    22441 gtgacctgac cgacaactac agcgtcaatg cccagtgcgg accatgcagc tggttgtact
    22501 tcctgcgcac tacccggctg ctgtggcaga cgagcgcgat ctaacggatt aaatttaccg
    22561 ctgttacgca agtcagcagc aacgatgccg ccaatatctt caggtgccgc accaggcccc
    22621 gcccactgga aaggaacaac accaatagga cgaccggaat ctacaccgct gtcgatcaca
    22681 atgcggactt cagcatgcag aactgatgcc cacagtatga gaaaaccaaa tgctactcgt
    22741 aatgcctgct tcatcatatc tcccttatct ggaccgatgg cccacgataa tttagcagaa
    22801 tgttaacaaa ctaaaataca caaaactacc agaaccccgt ggcaaccggt gcctgatgtt
    22861 gaccgtccga acagtcaaca tcgcgattac ggtttgaagt ccaatggcgc gtttttgaac
    22921 acttcatata ctgcctggct tggtggtttc gggatcttcg caagtttagc tgctgccaac
    22981 gcagcctgac aaagtgcggg atcgccacct tcaggtttga tatccagtaa cataccatcg
    23041 ggtgccagtt ttatgcgcag cgtacaggtt ttgcctgcat aggacgatgc gtcatagaac
    23101 ttactttcga tagcagattt aatctgcccg gcatagttat tgatatcggc ccctgatgcg
    23161 ccattgtttt tagtattacc actcccggca ggcgaagcat tgttcccttt cgcccctccc
    23221 cccgttttcg gtgcattctt accagagctt agctcaccga aaatatcatc tgcctctgcg
    23281 gccgcttttg ctgcagcggc tttttcagct gcagcttttg ccgctgccgc ttttttgtct
    23341 gcggcggctt tttctgccgc tgctttctta tcagctgctg ccttttcagc agccgctttt
    23401 tctgctgctt ttttgtcggc tgcagctttc tctgctgccg ctttcttatc agctgcagcc
    23461 ttttcagcag ccgctttctt ctcagcttct gctttggctt tttcagcagc ttcagttgcc
    23521 gctttctttc ttgcttcagc tgcagctgct tctgccgctt ccgctttctt cttcagtgcc
    23581 gcagcggctg cctcggcttt tttctgcgct tcggctgcgg ctttggcggc ttctgcttct
    23641 gcttttttct ttgcgtctgc agccgctttc ttcgctgctt cttccgcagc tttagcatct
    23701 gcttcggcct tcgctttagc atctgccgcc gctttcgctg ccgcctcttc agcttgcttc
    23761 tgctttaact cggcctgttt tgcggcttct tcagcctgct ttttctgctc ctgagccgct
    23821 aaccgctctt tctcaagttg cttcaggcgt tcctgttcag ccgcttgttt ctcacgcagt
    23881 tcttcagcag cctgctgttc cttcatcttg cgctgttcat cagaacgctt cgcgcttgat
    23941 tcctggcttt gcatgcgttt gtactgctca actaccgcac ctgaatcaac catgacagcg
    24001 tcgatggacg aaccaccgcc gcctccggct gaagcttcta tattctcatc gaacgaactc
    24061 cagatcagcg ccgcaaataa gatgacatgc agcactgctg aaataattat cgcccgcttg
    24121 agcttgtcgt tttgttcggt tgcctttgac actctcggtt tccaaaaact gttcgcctgt
    24181 tacccgctct ctttcaagca agggaaacgc agatgtttag ataggctgcg tcattaaacc
    24241 aaccgatttc acacccgcac tatgtaacaa gttcagtgct ttaattattt catcgtaagg
    24301 cacatctttt gcgccaccga tcagaaagac cgttttcggg ttggccttga aacggctgga
    24361 cacttccgcc accacctgct ctggtggtaa acgctccagg cgatctttct caaccaccac
    24421 ggtgtactga ccaataccag acacttcaac aatcactggc ggattatcgt tactgctcac
    24481 cgcctgtgat tcagtagcgt ctggcagatc gacctccacg ctctgggtga tgatgggcgc
    24541 tgtcgccatg aagatcagca acagcaccag cagtacgtcc agcaacggta caatgttgat
    24601 ttcggactta agttcgcgac gacctcgtcc acgcgctctg gccatgactt accccttatt
    24661 gctctcgcta acggtaaacg cctggcggtg cagaatcgcg gtaaactctt ccataaagtt
    24721 gtcgtaattc agttccagtt tgtttacgcg ctggttgagg cggttgtagg ccataactgc
    24781 cgggatagcg gcaaacagac caattgcagt cgcaatcaac gcttctgcga taccgggcgc
    24841 aaccatttgc agtgttgctt gttttaccgc cccgagggcg ataaaggcgt gcatgatccc
    24901 ccagaccgta ccaaacagac caatatacgg gctgatggag ccaaccgtac cgaggaacgg
    24961 aatgtgcgtt tccagatttt caagttcacg gttcatggag atacgcatag cacgcgacgc
    25021 cccttccact acggcttccg gcgcatggct attggcacga tgcaggcgca caaactcttt
    25081 gaacccgctg taaaatattt gttccgaacc agtcagatta tcccgtttcc cctgactctc
    25141 ttgatagagg cgagagagtt cgattccaga ccagaattta tcttcaaacg cttcggcttc
    25201 gcgcgccgct gcgttaagaa tacgggtccg ctggataata atggcccagg atgcgattga
    25261 aaaaccaatc aaaatcaaca tgataagttt aaccagaagg ctagccttca ggaacaaatc
    25321 aaggatattc atgtcagtca ctgcttaaac tccgcgacaa tagacttggg aagcgcacga
    25381 ggcttcattt tgagtgggtc aacgcaaaca accagaacct ctgcttcatt cagcaaagta
    25441 ttctcggcgt tgacaatacg ttgcgtgaaa accaaagagg tgccacgcat tgatgttatt
    25501 tcagtctgta tttcgagcat atcgtcgagc cgcgcaggtg cgtaatattc caccgtcatt
    25561 ttacgtacca caaaggcaac gcgttcagcc atcagcgcct gctgactgaa gtgatgatga
    25621 cgcagcatct ctgtgcgtgc tctttcataa aaagcgacgt aactggcgtg gtacaccaca
    25681 ccaccggcat cggtatcttc atagtagacg cgaaccggcc atcgaaacag cgttgtattc
    25741 actttacatc ccggtaatgc aacaaaagtt agagctttta aacgctgcta ctatacgcgc
    25801 gcgattgagg tttgggaatg agggcaagtt aagggagcgt aaaaatttat gggcctggag
    25861 cggcccataa ttttttaaag agaattctga tttaaaagaa gaagaaaatc agcccgacaa
    25921 tgagaacaat atcggcaagc aatgggcaaa aaatcccttg ccaaagaacc ttctgcggac
    25981 gaaagcccac gccgtgaatc acaccagcac agacggccca catcagcaat aaaccatgcc
    26041 agatttccag ttcactggtc ttcgcggcaa aacgagatgg gtcccaaaac atacatcctg
    26101 ctaacagaag cgccatcacg aaggaaagcg cccgcagggg gcgcttgtcc attaccgcat
    26161 acaaagtcgc gataatctta ctcatcagat gtcttcttga ccggctttgc ctgattcgac
    26221 gtgttcaagc gccagcgcgg tgattacccc aaacgaacag gcaagaagcg ttcccagaat
    26281 ccatgcgaaa taccacattt ttagctcctt acttagtaca gagagtgggt gttacgttca
    26341 atatcttctt tggtgatacg accgaacatt ttccagtaac accaggcggt gtagagcaga
    26401 atgatcggta ccagaaccac cgcaacccag gtcatgacgt taagcgtcag ctggctggaa
    26461 gttgcatccc acattgtcag acttgcgttc atcatggtgc tggacggcat cacaaacggg
    26521 aacattgcga taccggctgt caggatgatg caggccagcg tcagggagga gaacacaaac
    26581 gcccacgcgg ctttatccat acgtgcagtc aggatggtca gcagcggcag aaccacaccc
    26641 agtgccggaa tagcccacag aattggcgtg ttgttgaagt taaccagcca tgcgccagct
    26701 tcacgaacca cttctttatt cagtgggtta gaggctgcgt aatggtccat tgtcgatttc
    26761 acgacataac catcgatacc gtacatcacc catacgccag ccagtgcgaa acagaccagt
    26821 gtcaccagcg cagccacctg agccgttgca cgggtacgca ggtgcagttc gcccacggta
    26881 cgcatttgca gataggttgc gccctgagta atgatcatcc ctacgctcac cacgcctgcc
    26941 agcaggccga acgggttaag caactggaag aagttaccgg tgtagtacag acgcagatat
    27001 tcatcaacgt tgaacggtac gccctgcaac aggttaccga acgctacacc aattaccagc
    27061 ggcggaacga agctaccaat gaagatgccc cagtcccaca tgttacgcca gcgggtttct
    27121 tcaatcttgg agcggtagtc aaaaccgacc ggacggaaga acaaagacgc cagcacgagg
    27181 atcatcgcca catagaagcc ggagaacgca gcggcataga ccatcggcca ggcagcaaag
    27241 agtgcgccgc ccgcggtgat cagccaaacc tggttaccgt cccagtgtgg tgcaatggag
    27301 ttaatcataa ttcgacgctc ggtgtcgtta cgaccgagga aacgggtgag catgcccacc
    27361 cccatgtcga aaccgtcagt gactgcaaaa ccaatcagca gaacgccaac cagcagccac
    27421 cagataaaac gcaatacttc ataatcgatc atttgacgac tcctgtctta gcgtgccggc
    27481 tgagtagtcg tggaagactg ctcaaagtga tagcgaccgg ttttcaggct gcttgggccg
    27541 aggcgtgcaa atttgaacat taagaacaat tctgccacca ggaacagggt atacaggccg
    27601 caaatcagca ccattgagaa gatgagatcg cctgcggtca gtgacgagtt cgccacagct
    27661 gtcggcagca cttcaccgat agcccacggt tggcggccat attcagccac gaaccagccc
    27721 gcttctacag caatccacgg cagcggaata ccgtacagcg cggcgcgcag aagccatttt
    27781 ttctcgccaa tgcggttgcg gatgacactc cagaaagaga gcgcgatgat tgccagaagc
    27841 aggaagccac acgccaccat gatacggaac gcaaagtaca gcggcgctac acgcgggatg
    27901 gagtctttgg ttgcctgttg aatctgcgct tcagtcgcat cagccacgtt tggcgtatag
    27961 cgtttcagca gcagaccgta accgaggtct ttcttcatgc tattgaactg gtcacgaacc
    28021 gcctggtcgg tagaaccaga acgcagttgt tcgagcagag agtacgcctt catcccgtta
    28081 cgaatgcgtt cttcatgctg caccatcagc tctttcaggc cgataaccgg ggtatccacg
    28141 gaacgcgttg caatgatgcc cagtgcgtaa gggatctgaa tcgcaaattt gttcgtctcc
    28201 tcttcctgat caggaatgcc gaacagagta aaggcagcag gcgcaggttg cgtttcccac
    28261 tcggcttcaa tagcagccag tttggttttc tgcacgtcgc ccatttcgta gccggattca
    28321 tcacccagaa caataacaga cagaacagca gccataccga agctggcagc gatagcaaag
    28381 gagcgtttag cgaaggcgaa gtcacgacct ttcagcatat accatgcgct gataccgagg
    28441 atgaacatcg cgccagtcac ataaccagac gctacagtgt gaacgaattt cacctgagca
    28501 accgggttaa gcaccagctc ggagaagctc accatctcca tacgcatagt ttcaaagttg
    28561 aaatcggacg cgattgggtt ttgcatccag ccgttcgcaa ccagaatcca cagtgcggac
    28621 aggtttgaac cgagcgccac cagccaggtg acacacatat gctgaacttt acccagacga
    28681 tcccaaccga agaagaacag acctacaaag gtggattcga ggaagaaggc catcagacct
    28741 tcgattgcca gcggcgcacc gaagatatcc cctacatagt gggaatagta agaccagtta
    28801 gtcccgaact ggaactccat ggtcagaccg gtagccacac ccagagcgaa gttgatacca
    28861 aacaacttgc cccagaactt ggtcatatct ttataaatct gtttgccgga gaggacgtag
    28921 accgtttcca taatggccag caggaacgcc ataccgagcg tcagtggcac aaaaaggaag
    28981 tggtacatcg cggtcaaggc aaactgtaag cgcgacagtt cgactatatc taacatcatg
    29041 actccttgct catcgcatga agactccgag agtgaacccc gttagaaagg gtcacacgca
    29101 tgccccaata gaaatttgtt tgaacacttt catcatacca tgtgaatgca cacaaaaacg
    29161 gcgtaaagaa aggttgcaaa catgttaata aaaactcaaa ttgatcccac gtatatatta
    29221 cgccgcaaaa tccttacaat aaacaggttt ttgttgaagc aaatttgcat ttttcgacgg
    29281 tgatcaattt atagctaaat taccgccttt cagccaattt gatcgagaac aatttatctc
    29341 tttttgatgc ccatttccaa gacttataca ttgataaata tcaattccta caaattatgt
    29401 taatcatgtt tcaataaggt aactttagtg acatttatgt ttaaaatgtg tgagttataa
    29461 ggctgaaatt agtttcggtt ggcaattaaa gataaaaaga gacgaagcaa aaacagcacg
    29521 gttgac
//
LOCUS       DAZKGS010000047        29149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKGS010000047 DAZKGS010000000
VERSION     DAZKGS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            260..844
                     /gene="yadN"
                     /locus_tag="ACHOQY_002002"
     CDS             260..844
                     /gene="yadN"
                     /locus_tag="ACHOQY_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9678110.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            949..1689
                     /gene="yadV"
                     /locus_tag="ACHOQY_002003"
     CDS             949..1689
                     /gene="yadV"
                     /locus_tag="ACHOQY_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9678111.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1724..4324
                     /gene="htrE"
                     /locus_tag="ACHOQY_002004"
     CDS             1724..4324
                     /gene="htrE"
                     /locus_tag="ACHOQY_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFP9678112.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4341..4910
                     /gene="yadM"
                     /locus_tag="ACHOQY_002005"
     CDS             4341..4910
                     /gene="yadM"
                     /locus_tag="ACHOQY_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9678113.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4925..5527
                     /gene="yadL"
                     /locus_tag="ACHOQY_002006"
     CDS             4925..5527
                     /gene="yadL"
                     /locus_tag="ACHOQY_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP9678114.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5554..6150
                     /locus_tag="ACHOQY_002007"
     CDS             5554..6150
                     /locus_tag="ACHOQY_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9678115.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6134..7477
                     /locus_tag="ACHOQY_002008"
                     /pseudo
     CDS             6134..7477
                     /locus_tag="ACHOQY_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7590..8384
                     /gene="panB"
                     /locus_tag="ACHOQY_002009"
     CDS             7590..8384
                     /gene="panB"
                     /locus_tag="ACHOQY_002009"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9678116.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8396..9247
                     /gene="panC"
                     /locus_tag="ACHOQY_002010"
     CDS             8396..9247
                     /gene="panC"
                     /locus_tag="ACHOQY_002010"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9678117.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9321..10223)
                     /gene="rpnC"
                     /locus_tag="ACHOQY_002011"
     CDS             complement(9321..10223)
                     /gene="rpnC"
                     /locus_tag="ACHOQY_002011"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP9678118.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10497..10877
                     /gene="panD"
                     /locus_tag="ACHOQY_002012"
     CDS             10497..10877
                     /gene="panD"
                     /locus_tag="ACHOQY_002012"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9678119.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10881..12110)
                     /gene="yadE"
                     /locus_tag="ACHOQY_002013"
     CDS             complement(10881..12110)
                     /gene="yadE"
                     /locus_tag="ACHOQY_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9678120.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12174..12614)
                     /gene="yadI"
                     /locus_tag="ACHOQY_002014"
     CDS             complement(12174..12614)
                     /gene="yadI"
                     /locus_tag="ACHOQY_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9678121.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12719..13489)
                     /gene="yadH"
                     /locus_tag="ACHOQY_002015"
     CDS             complement(12719..13489)
                     /gene="yadH"
                     /locus_tag="ACHOQY_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9678122.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13486..14412)
                     /gene="yadG"
                     /locus_tag="ACHOQY_002016"
     CDS             complement(13486..14412)
                     /gene="yadG"
                     /locus_tag="ACHOQY_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9678123.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14521..15183
                     /gene="can"
                     /locus_tag="ACHOQY_002017"
     CDS             14521..15183
                     /gene="can"
                     /locus_tag="ACHOQY_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9678124.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15224..15760)
                     /gene="hpt"
                     /locus_tag="ACHOQY_002018"
     CDS             complement(15224..15760)
                     /gene="hpt"
                     /locus_tag="ACHOQY_002018"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9678125.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15966..18356
                     /gene="gcd"
                     /locus_tag="ACHOQY_002019"
     CDS             15966..18356
                     /gene="gcd"
                     /locus_tag="ACHOQY_002019"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9678126.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWSYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18403..19953)
                     /gene="cueO"
                     /locus_tag="ACHOQY_002020"
     CDS             complement(18403..19953)
                     /gene="cueO"
                     /locus_tag="ACHOQY_002020"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9678127.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20119..20466
                     /gene="yacC"
                     /locus_tag="ACHOQY_002021"
     CDS             20119..20466
                     /gene="yacC"
                     /locus_tag="ACHOQY_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9678128.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20572..21438
                     /gene="speE"
                     /locus_tag="ACHOQY_002022"
     CDS             20572..21438
                     /gene="speE"
                     /locus_tag="ACHOQY_002022"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9678129.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21454..22248
                     /gene="speD"
                     /locus_tag="ACHOQY_002023"
     CDS             21454..22248
                     /gene="speD"
                     /locus_tag="ACHOQY_002023"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9678130.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22286..22648)
                     /gene="yacL"
                     /locus_tag="ACHOQY_002024"
     CDS             complement(22286..22648)
                     /gene="yacL"
                     /locus_tag="ACHOQY_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9678131.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22823..25420)
                     /gene="acnB"
                     /locus_tag="ACHOQY_002025"
     CDS             complement(22823..25420)
                     /gene="acnB"
                     /locus_tag="ACHOQY_002025"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9678132.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25775..27436
                     /locus_tag="ACHOQY_002026"
     CDS             25775..27436
                     /locus_tag="ACHOQY_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9678133.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSASPEQRQ
                     VFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(27507..28931)
                     /gene="lpdA"
                     /locus_tag="ACHOQY_002027"
     CDS             complement(27507..28931)
                     /gene="lpdA"
                     /locus_tag="ACHOQY_002027"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9678134.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
BASE COUNT         7524 a         7443 c         6912 g         7270 t
ORIGIN      
        1 gataaatatc aatcccatcg ccatacccta accaattgca cttgtgaatt tatttttgct
       61 taattttgaa ttaagaaaat ttaatttgtt caaaagccct taaagtgcac aataggcgtc
      121 ctaatgaaca atggttgtaa attttggtaa caaatatcat tgctcattaa tatctagcat
      181 ctgcttataa catttgtgtc atgaatagtc attcttcaaa ccagtagtct gactaatgga
      241 attttattca aggattttta tgtctaaaaa attaggtttt gccctgtctg gtcttatgct
      301 ggcaatggtt gcaggtactg cttctgctga tatggatggt ggtcagttaa atatcagtgg
      361 tctggttgtt gataacacct gtgaaacccg cgttgacggc ggtaataaag atggtctgat
      421 cctgctgcaa actgcaactg ttggtgaaat cgatgctggc gtgctgaatg acaccgttgg
      481 cgctaaagcc aaacctttca gcatcaccgt tgactgcagc aaagcaaatc cgaatccagg
      541 tagtacagct aaaatgacct tcggttctgt tttctttggt aatagcaaag gaacgctgaa
      601 caacgacatg tctattaaca acccgtctga cggcgtgaat atcgctctgc acaatattga
      661 tggttccact atcaaacagg ttcaaattaa caaccctggc gatgtgtata ctaaagccct
      721 ggatgcaacg acaaaatctg ctgtttatga ttttaaagcg tcttacgttc gtgccgtcgc
      781 agaccaaaca gcaactgctg gttatgtaaa aactaacact gcatacacca ttacttatca
      841 gtaagtattc accaattaaa ttgattgaat gaatatacag ggaatagtgt taataatttc
      901 tatttttata ttattccctg ttttaattaa ctctgtcagg gatggtttat gttttttaac
      961 accaaacata caacagcttt atgcttcgta acctgtatgg cttttagttc atcgtctatt
     1021 gcggacattg tcatttcggg tactcgcgta atatataaaa gcgatcaaaa aagtgtcaac
     1081 gtacgtctgg aaaataaagg gaataacccg ttgcttgtcc agagttggtt agatactggc
     1141 gatgacaacg ctgagcctgg cagtattaca gtccctttta ctgccacgcc gccagtatcg
     1201 cgtattgatg ccaaacgtgg gcaaacaatc aaattaatgt acacagccag cacctcactg
     1261 cctaaagaca gagagagcgt gttctggttt aacgtactgg aagttccacc aaaaccagat
     1321 gcagaaaagg tcgcgaatca aagtctgctg caactggcat ttcgcacacg tataaaactt
     1381 ttctatcgcc cggatggatt gaagggaaat ccctctgaag ccccgttagc cctgaagtgg
     1441 ttctggtcag gttcagaagg taaggcgtca ttgcgagtga ccaatccaac cccttactac
     1501 gtctctttta gcagtggtga tttagaagct agcggtaaac gctatccgat tgatgtgaaa
     1561 atgattgcac catttagtga tgaggtcatg aaagtcactg gccttaatgg caaagcaagt
     1621 tctgcaaaag tacattttta cgccattaat gattttggtg gcgccattga aggtaatgcc
     1681 agtctgtaat caggcaagga tataattccg caggaagcat aacgtgacta taaaaccaac
     1741 taaaaattat cataatcatc tgacccgtat cgctacgttt tgcgcgctgc tgtattgcaa
     1801 tagtgctttc tgtgctgaac ttgttgaata tgaccatacc ttcctgatgg ggcagaatgc
     1861 ctcaaatatt gatctcagcc ggtatagtga aggtaaccct gctataccgg gtatgtacga
     1921 cgtcagtgtt tatgtcaacg atcaacctat tattaaccaa agcattacat ttattgaaat
     1981 tgaaggaaaa aaaaacgcac aagcctgtat cacacttaag aatttattgc agtttcatat
     2041 aaattctccc gatataaata acgaaaaagc cgttctgctt gccagggatg aaacactcgg
     2101 caattgcctt aatttgacgg aaattatccc tcaggcttct gttcgttatg acgttaacga
     2161 gcaacgtctg gatatagacg ttcctcaggc atgggtaatg aaaaattacc aaaactatgt
     2221 tgatccatcg ttatgggaaa atggcattaa tgcggccatg ttgtcataca acctcaacgg
     2281 atatcatagt gaaactcctg gtcgaagaaa tgacagcatt tatgctgctt ttaacggtgg
     2341 gatgaattta ggtgcatggc gactgcgtgc ctcaggcaac tacaactgga tgaccgattc
     2401 tggcagtaat tatgatttta agaatcggta tatacagcgt gatatcgcct cgctgcgttc
     2461 tcaactcatt cttggtgagt cttatacaac gggcgaaacc tttgattctg tcagtatccg
     2521 cggcattcgt ttatacagcg acagccgcat gttgcctccg acattagcga gttttgcgcc
     2581 tatcattcat ggcgttgcta ataccaacgc caaagtcact attacgcaag gtggctataa
     2641 gatttatgaa acgactgtgc cgccaggcgc tttcgtcatt gatgatctga gtccgtcagg
     2701 ttacggcagc gatcttattg ttactgtcga agaatccgat ggctcaaagc ggacattctc
     2761 gcaacctttt tcatcggtcg ttcaaatgtt acgccctggc gttggacgtt gggatattag
     2821 cggcggccag gtcttaaaag atgatattca ggatgagcct aatttatttc aggccagcta
     2881 ctactacggt ctgaataact atctaacggg ttatactggt attcagatta ccgataataa
     2941 ctataccgct ggtttgttag gtcttggtct gaatacttca gtcggtgcat tttctttcga
     3001 tgtgactcac tccaatgttc gtataccgga tgataaaaca tatcagggac aaagttatcg
     3061 tgtttcctgg aacaagttat tcgaagaaac aagtacttca ctgaatatcg cggcctatcg
     3121 ctattcgaca cagaattacc ttggtcttaa tgatgcacta actctaattg atgaagtgaa
     3181 acatcccgaa caagatcttg aaccgaaatc catgcgtaat tactcgcgca tgaaaaatca
     3241 ggttacggtc agtattaacc aaccgctaaa atttgagaaa aaagattacg gttcatttta
     3301 tctttccgga agttggtccg attactgggc ttccggacaa aatcgtagca attactctat
     3361 tggctacagt aacagtgcat cctggggcag ctacagtgtc agtgcccagc gttcatggaa
     3421 tgaagacggc gacactgacg atagcgttta tctcagtttc accattccta ttgaaaaatt
     3481 acttggcact gaacaacgga cttcaggttt ccagagtatt gatactcaaa tgagcagtga
     3541 ctttaagggt aataaccaac tcaacgtcag tagcagtggc tatagtgata acgctcgcgt
     3601 cagttatagc gtaaatactg gctatacgat gaataaagcc agcaaagatt tgagttatgt
     3661 tgggggttat gccagctatg aatcaccgtg gggaaccctg gcaggttcag tttctgcaaa
     3721 tagcgataac agtcgtcaag tgtctctcag caccgacggt ggttttgtat tgcatagcgg
     3781 cggcctgacc ttcagtaatg acagttttag cgactccgat acactggcgg tagttcaggc
     3841 tccaggtgct caaggagcgc gaataaatta tggcaacagt actatcgatc gatggggtta
     3901 tggtgtcacc agcgctcttt ctccttatca tgaaaaccgt atcgcgctgg atatcaacga
     3961 tcttgagaac gatgttgaat taaaaagtac cagtgcagta gctgtaccgc gtcagggttc
     4021 agtcgtcttt gctgattttg aaaccgtgca agggcaatca gccattatga acatcacacg
     4081 aagtgatggt aaaaatattc catttgctgc agatatttat gatgagcaag gcaatgtcat
     4141 tggtaatgtt ggacagggtg gacaagcatt tgttcgtggt attgagcagc agggaaatat
     4201 cagcattaaa tggctcgaag aaagtaaacc cgtaagttgt cttgcgcatt atcaacaaag
     4261 ttcagaagca gaaaaaatag cgcaatctat tattctgaat ggaattcggt gtcagattca
     4321 gtaactacaa gggacgtcaa atgataaaaa taacgccaca taaaatagcg atactgatgg
     4381 gaatattatt atcaccatca gtatttgcaa cggatattaa tgtagagttt acagccactg
     4441 tcaaagcgac aacctgtaac atcacactta ctggtaataa cgtcacgaat gatggcaata
     4501 ataactacac attgagaatc cctaagatgg gtctggataa gatcgcgaat aaaacgacag
     4561 aatctcaggc tgattttaaa ctggttgcta gtgggtgcag cagtggcatc agttggattg
     4621 ataccactct gagcggaaat gcatcatcaa gctcacctaa gcttattata ccgcagtctg
     4681 gtgattcatc ttcgacgaca agtaatatcg gtatgggttt caaaaaacgg actactgatg
     4741 atgccacttt ccttaaacct aacagtgcgg aaaagatacg ctggagcaca gacgagatgc
     4801 agcccgataa gggtcttgaa atgaccgttg cgctacgtga aacagatgca gggcaaggcg
     4861 taccggggaa ttttcgtgca ctggcaacgt ttaatttcat ctatcaataa gaaggataat
     4921 taagatgaca tttaaaaatt tacgttatgg attatccagc agtgtcgttt tggctgcctc
     4981 attgttcagc gtaccctctt atgcggcaac agacagcatt gggctgaccg tgattactac
     5041 cgtagaaata ggtacttgta ccgctacatt agtaaatgac tctgatcagg acatttctgt
     5101 tgttgacttt ggcgatgtat atatttctga aatcaatgcc aagaccaaag ttaaaacatt
     5161 caaactcaaa ttcaaagact gtgcgggtat ccccaataaa aaagcacaaa taaaattaac
     5221 caagcgagcc acatgcgaag gtactgctaa tgacggtgcg gggttcgcaa atggatccac
     5281 agccgcagat aaagcaagtg ccgtcgccgt tgaagtctgg agcaccgaaa ctccggcaac
     5341 agggagcgca acacaattta gttgtgtgac accagcatct caagaggtca ctatctccaa
     5401 tgcagccaac gcggttgttt attatccgat gagtgctcga ctggtcgtcg aaaaaaataa
     5461 aaccgtaagc aatgtcactg cgggtaaatt ttctgcacct gccacattta cagtaaccta
     5521 taactaacag actgacagat ccaggaacta tatatgcatc ccactcaacg taagctgatg
     5581 aagagaataa ttctgtttct gtcactacag tttcccatta cctatccagc catcgctggg
     5641 caggatattg accttgttgc caatgtaaaa aacagcactt gcaaaagcgg aatcagtaac
     5701 cagggtaata ttgatcttgg cgtcgttggg gtgggatatt tttcagataa tgttactcct
     5761 gaaagctatc aaccaggtgg aaaagagttc actgtcacag tatcagactg tgcattacag
     5821 ggaactggcg atgtgcttaa tcagttacat attgatttta gagcccttag cggtgtcatg
     5881 gctgcaggtt caaggcaaat atttgccaat gaagtcgcaa ccggggctaa aaatgtcggt
     5941 gtcgttctct tttccattca ggacccgacc aatatattca atgttattag ttcagcagga
     6001 aattctcgtt ccgtgtatcc agtaatgact tctgccctgc ataattcttc atggaaattt
     6061 tatgccagaa tgcaaaaaat cgatcccgcg ctggatgtca tatctggcca ggtaatgagt
     6121 catatattag ttgatgtgta ctacgaataa tagatcaaaa caaataactg cgactgttac
     6181 agcagtagat ggtggagtga taatgaagaa attcttcaga cattttcttt ttttaattct
     6241 gtgcttgagt tgttataccg cgagtgctgg cactgatgat aacgtaggat atattgttgg
     6301 gaatagttac ggcgttggtc cgtcagatca aaaatggaga gaaacggggc cgaatggcga
     6361 tgctactgtc atattcagat atgcaacaag tacaaataat ttagttttct acaaaccgac
     6421 gcagcttgga cctacaggtg taaaattaca gtggagtcag ttagataccg cttctggtgg
     6481 tggttttctt tattgcaaca gaagtgatag cacaagtggt agcgcaatgc gtattgaaaa
     6541 tgcaatggtt gactcaggta aaatgtatgg ctcccataaa ttatttaata catcagttcc
     6601 tggtttgtat tacacattat taatttcaaa catgtggtca gcttacggta ccgtaactaa
     6661 cgttagttca cctgggatat acattggtga ctctgcagaa caatattttt cgtggtataa
     6721 tccaagcgaa gacgtgttat attagagttg caataatgcg aatagcaccc gtaaatactg
     6781 ggctgtaggt ggtatttatc agacccttac aattgaattc tatacagata caaactttga
     6841 tccaacagta actcaacaga tcaaattatc cagctcatca aattatctgt attcatttaa
     6901 agcctatggc gcaggccaag gtataaatga gcatagttat tttatcaaaa tcgatttcga
     6961 tctgctcaat gtcaagttga ctaatcccac ttgttttacc gctatgctta gtggaacttc
     7021 tgtgactggt tctacggtaa aaatgggtga atatagtgca gaacaaatca gaaacggtgc
     7081 cacaccggtt ccttttgata tttcacttca aaactgcgtt cgtgtgacta atattgagac
     7141 aaaattagtt tcaacaaagg ttggtactga aaacgggcaa ctccttggta atactctcac
     7201 aggtaatgac gcagcaaaag gagtcggtgt actcatagaa ggtttagcaa ctagtaaaaa
     7261 tcctctaatg acattgaaac ctaatgattc aaattctgtt tataaagatt acgacccgag
     7321 aggcaaagac gatacaacag gaggagttta cccggatcaa gatacaggta taacataccc
     7381 tctccatttc caggccacgc tacaacagga tggaactata ccaatagaag ctggtgagtt
     7441 taaagccacc agtactttcc aggtaaccta cccttaataa gtccaccgca ccgccatcat
     7501 ctggcggtgc agtaattgat aaatcattgc ccccaccaat gtgactgata aaatgccagg
     7561 caacacgaca tcatttatca ggatacgtta tgaaaccaac caccattgct tcacttcaga
     7621 aatgtaagca ggataaaaag cgtttcgcga ccatcaccgc ttacgactac agcttcgcga
     7681 aactatttgc tgatgaagga cttaacgtca tgctggtagg cgactcgctg ggcatgacgg
     7741 ttcagggtca cgactccacc ctgccggtga ccgttgctga tatcgcctac cacactgccg
     7801 ccgtacgtcg cggcgcgcca aactgcctgc tgctggctga tctgccgttt atggcgtatg
     7861 ccacgccgga acaagccttt gaaaacgccg caacggttat gcgtgccggt gccaatatgg
     7921 tcaaaattga gggcggtgag tggctggtcg aaaccgtaaa aatgctgacc gaacgtgccg
     7981 ttcctgtatg tggtcactta ggtttaaccc cacagtcagt gaatattttc ggtggctaca
     8041 aagttcaggg gcgcggcgat gaagcgggcg atcaactgct cagcgatgca ttagccttag
     8101 aagccgctgg ggcacagctg ctggtgctgg aatgcgtgcc ggttgaactg gcaaaacgta
     8161 ttaccgatgc actggcgatc ccggttattg gcattggcgc aggcaacgtc actgacgggc
     8221 agatcctcgt gatgcacgac gccttcggca ttaccggcgg tcacattcct aaattcgcta
     8281 aaaatttcct cgccgaaacg ggcgacatcc gcgcggctgt gcggcagtat atggctgaag
     8341 tggagtccgg cgtttatccg ggcgaagaac acagtttcca ttaaggagtc acgttgtgtt
     8401 aattatcgaa accctgccgc tgctgcgtca gcaaattcgc cgcctgcgta tggaaggcaa
     8461 gcgcgtggcg ctggtgccta ctatgggtaa tctgcacgat ggccatatga agctggtcga
     8521 cgaagccaaa gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc caatgcagtt
     8581 tgaccgcccg gaagatctgg ctcgttatcc acggaccttg caagaggact gcgaaaagct
     8641 aaacaaacgt aaagtggatt tagttttcgc cccttcggta aaagagatct acccgaacgg
     8701 tactgaaacc cacacttacg ttgacgttcc tggcctttcg accatgctgg aaggtgccag
     8761 ccgtccggga cattttcgcg gcgtttcgac tattgtcagc aagctgttca acctggtcca
     8821 gccggacatc gcctgcttcg gtgaaaagga ctttcagcaa ctggcgctaa tccgcaaaat
     8881 ggttgccgat atgggcttcg atattgaaat tgtcggcgtc ccgattatac gcgccaaaga
     8941 tggccttgcg ctcagttccc gtaatggtta tctgacggcg gaacaacgca aaattgcgcc
     9001 cggtctgtac aaagttttaa gttcaattgc cgacaaattg caggctgggg aacgggatct
     9061 cgatgaaatt attaccattg cggggcaaga actgaatgaa aaaggcttcc gcgccgatga
     9121 tattcagatt cgcgatgccg acacattgct ggaagtttct gaaaccagca aacgggcagt
     9181 aattctggta gccgcctggc ttggcgatgc tcgcctgatc gacaacaaaa tggtcgagct
     9241 ggcgtaatac ttaactggcg ctacggctga tggcgccagt tattaattta ccccacgtca
     9301 tctgtcataa cacaggtaac ttaaataagg ttaattacct tatcaatctc agcaagaggt
     9361 aaatttgcca tctctgcaac gtcttcccga gacattcctt tacttagaag acgcagggcg
     9421 aattcttgcc tttcctcctg tcttccctgc tgaatcccct tctcaatccc tttctcttca
     9481 aaccactgcg ccagcgtcat catagactcc cctcccgttt ccctgtctct cagcacacca
     9541 taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg catggcaact
     9601 aactgacttc cgctagtgta cccttcgtca atcagcgtga ccagttgctc cagcaataac
     9661 attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat ccgccgatgt
     9721 tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg actgttatag
     9781 acgcgtcgtg ccagctccgg cgagtaaaat atatcaaacc agcacattga gagcggataa
     9841 ggtgtggcct cgccctgata aaacagtatc ggcaccacca gcggcagctt atcgtgatcc
     9901 gcctccagat gccggtgcat ggcagcaata gaataacgca tcatgcgaaa ggccattttc
     9961 ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt gccctgcatt
    10021 tgcacggaat agagcacgtc cgtgctgtgt cctttcaggc tctcttcaat gaaactcccc
    10081 gactctaaat gaagcgtgtt gaggtcacaa agttctcgta attccactgg caaatgtatc
    10141 tccagaaagt cgcgagccgt ctccgcgtgc attaaaaatt gtttaaacac tgcatcatgc
    10201 ggtgtggtac tcggtgcatc catcttccct gccttccgtg ttgtcttgcg tgaaggcagt
    10261 atccagtcaa ggcgtgccat cgtcagccac ctctttagtg tttaccgagc agcgttcaga
    10321 gaaatttcaa ttggttacag ccgttacatg gcacgcttcg gcacggtacg caaactgctg
    10381 atgtaaatgc cgtcagatga gtagacacta aacaaaaatc gggcaatact gcgtgagaat
    10441 ttcctcaaag caggcatcgc ctgcttcgtt aacgacaggg tagaaaggta gaagttatga
    10501 ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg gacctgcact
    10561 atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt ctcgaaaacg
    10621 aagccattga tatctggaat gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg
    10681 cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc gccagtgtcg
    10741 gcgatattgt catcatcgcc agcttcgtta ccatgccaga tgaagaagct cgcacctggc
    10801 gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg aaagcgattc
    10861 cggtacaggt tgcttgattg ttatccctgc ggctggttac tcaccagccg cgacatcgtc
    10921 tccagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt atcccccggt
    10981 tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt tgctgccttc
    11041 acggctttgt cattaaatcc gccaaacgga tacgaaagat accagacatg cggattaaat
    11101 tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc actacggctc
    11161 agtaatatgg gtcggcgata gccatccacc cgatgcaaaa aatgggtatg tgactgaaaa
    11221 tcaaaaacat cgcgaatttc gttaagctca gaaacgctca taaattgcag cgattttggg
    11281 ttccacttct gcgggtgacg tttgatgcgc gaggtaacaa caaacgccgt cgccttcatg
    11341 ccatattgtt tcaatacagg atacgcatag cggctcaccg acttgaggcc atcatcaaag
    11401 gtaatcacca ccgctcgcgc agggagattg atcttattct tcacgtaacc ttccagctgc
    11461 gccatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatttggtt attgaaagcg
    11521 cgtaccgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcatc gcgcagaata
    11581 tggtgatagg ttagcaccgg caggccatta tcgggttggg catccagcgc gctgatatag
    11641 gcgagtcgat cgccaatacg gatctgatac caggtttgat ttaacctgtc tttcagttta
    11701 tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa atggcgcact gcccgcactc
    11761 ggcgcgttat agaccggcgt atctttccag gtaattaagt tctgattact cagcggcttg
    11821 ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaactggctc gagatgccct
    11881 ttatcgataa accctttacc aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc
    11941 tccacggcaa tgatttgccc ggcccgaata ttccccacgg tcaccatctt gtcgccaatt
    12001 tgcgcccaga tcgcagcatt ttcgatggtt tgcatataac gggcaggtaa cgcggcactg
    12061 acacttgcgg taaacagcat cagcaggaga ataacagctt gtttgtacat gaaaaaataa
    12121 ccgagggtga aacaacgctt ttattgtagc aaaagcgcta taagaatcgt gggttaatac
    12181 tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgttggtg ccaaagacta
    12241 ctcacctccg gcccacccag ttcgacaata cgctcgcgaa actctgaact ggtcatggtt
    12301 tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat cacttcacaa
    12361 cgggagtgtt tatggcttaa taatgaagcc acgcggtacg gtggcgctcc ggctaaatcg
    12421 gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca catcatgcgg
    12481 ctgagcatat tagagcttaa tccgcgccag aaattcacgg cccggcactg aagaagtgcc
    12541 ccatgttttt tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt
    12601 acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga ccattgttga
    12661 taaagggtga tagccgtcaa aactcctctc ccccgaatcc agaggagagg aaatagcctt
    12721 agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa aacgccacaa
    12781 taaagaccac cagtacgcca aaggtagtga ccagcggcac atcatttata ccgaggaagc
    12841 cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc ccttgccaga
    12901 acggcggcaa caaagtcagg gagtaaaaca ccccgcccaa atacgtgagt ggcgttaaca
    12961 caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacgccg ttcagcaaac
    13021 ccgcaaggga gaatagcacc gccgtgagca ccagggttaa ggcaacgaat acccacgaat
    13081 gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca acaaacagac
    13141 cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga accggcgcta
    13201 ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac gcgacgttag
    13261 cgtaggcatt ggtgatcacc gacatcatga tcagccccgg tacgatgaac tgcatatagc
    13321 tgaagccatg catatcgcca atacgcgaac ctatcaggtt accgaagata ataaagtaaa
    13381 gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata aagcgatgga
    13441 tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg cgcgatctcc
    13501 ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt tgttacgcat
    13561 acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga tcccctgctc
    13621 acgcagcact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat agccatcgag
    13681 cttcggtaac ggacttttcg gtgcgagatc gagaataaag gtttccgatt tcagcttagc
    13741 cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa tgccgatatt
    13801 gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg tggtgccttt
    13861 gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat ccacgcctgc
    13921 ggtcggttcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg caatcattaa
    13981 acggcgcttc atcccgccag ataacatacg cgcacgttcg ttgcgttttc cccatagatc
    14041 gagctgctta agatattttt cgctgcggat gtacgcctct ttgcgctcca caccgtagta
    14101 ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt taaattcctg
    14161 cggcaccagt cccaactgac gtttagcgtt cacgacatcc ttctcgagat cgtaaccaaa
    14221 tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga tagtggtcga
    14281 tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc
    14341 tatcccacga agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt gaagttccag
    14401 tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt ggtttaaatc
    14461 gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc gttaacctcc
    14521 atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat gctggtggaa
    14581 gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt tctatggatt
    14641 ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc
    14701 tttgttcacc gtaatgttgc taacctggtc attcacactg acctgaactg cctttccgtg
    14761 gttcagtatg cagtggatgt actcgaagtt gaacacatta ttatctgtgg ccactacggt
    14821 tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa caactggctg
    14881 ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat gccgcaagag
    14941 cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa cctgggccac
    15001 tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca cggctgggcc
    15061 tacggcattc acgacggctt gctgcgtgat ctggatgtta ccgccaccaa ccgcgaaacc
    15121 cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc caaccacaaa
    15181 taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg tccagcagaa
    15241 tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg ccgtaaccca
    15301 ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc acttcacgac
    15361 gggacggttt atccagcagc gtacaaatcg ccagcgactt cggttcgcgc aggcttaaga
    15421 tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca atcagcacgt
    15481 ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg gtggtggaca
    15541 tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc tgaacttcac
    15601 ggcacaggtc cgccataaac ataaatgagc cacgcagcag acccaccagc accatatcgc
    15661 tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata cgcgctttaa
    15721 tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc atatgattta
    15781 aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa gacacgcagt
    15841 ataccagtaa aactattcat tcgcggaatc tgttaataaa gctcgaattg tgatgacgat
    15901 cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg aaatggtgtc
    15961 tctttatggc aattaacaat acaggctcgc gacgattact cgtcacgcta acagcccttt
    16021 ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc gcgattggcg
    16081 gctcctggtc ctaccctatc gctggccttg tgatgctcgg cgtcgcctgg atgctgtggc
    16141 gcagtaaacg tgccgcgctt tggctatacg ccgccctgct gctcggcacc atgatttggg
    16201 gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgat attctggtct
    16261 tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg
    16321 gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tagtatcctg acctgggccg
    16381 gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca cctgcggaag
    16441 ctatctcacc cgttgccgat caggactggc ctgcctatgg tcgcaatcag gaaggtcaac
    16501 gcttttcacc tctgaaacaa attaacgccg ataacgtcca taatctgaaa gaagcctggg
    16561 tgttccgtac tggcgatgtg aagcagccga acgatccggg cgaaatcacc aatgaagtga
    16621 cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcgc ctgtttgcgc
    16681 tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa actaacgagt
    16741 ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca gaaacggctt
    16801 cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccggtcaat gatggtcgac
    16861 tgattgcgat taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat aaaggcgtgc
    16921 tcaatctgca aagcaatatg ccagacacca aaccgggtct gtatgaaccg acttcgccac
    16981 cgattatcac cgataaaacc atcgtgatgg caggttcagt taccgataac ttctcaaccc
    17041 gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg ctgtgggctt
    17101 ttgatcccgg cgcgaaagat ccgaacgcaa tcccgtctga cgaacacacc tttaccttta
    17161 actcgccaaa ctcgtgggcg ccagcggcct atgacgcgaa gctggatctg gtctatctgc
    17221 cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa caggaacgtt
    17281 atgccagctc gattctggcg ctgaatgcca ctaccgggaa actggcgtgg agctaccaga
    17341 ccgttcacca cgacctgtgg gacatggatc ttccggcaca gccgacgctg gcggacatca
    17401 ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc aacatttttg
    17461 tgctcgatcg tcgcaatggc gaactggtgg ttccggcccc ggaaaaaccg gtgccgcaag
    17521 gtgctgcgaa aggcgattac gttactccga cccagccgtt ctctgaactg agcttccgtc
    17581 cgaagaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac caactggtgt
    17641 gccgcgtgat gttccaccag atgcgctatg aaggcatttt cacgccgcca tctgaacagg
    17701 gcacgctggt cttcccgggt aacctgggaa tgtttgaatg gggcgggatt tccgttgatc
    17761 caaatcgtga agtggcaatt gccaacccaa tggcactgcc gtttgtttcg aaactgatcc
    17821 cacgcggtcc gggtaacccg atggagcagc cgaaagatgc caaaggcacg ggtacggaat
    17881 ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg ttcctctcac
    17941 cgtttggtct gccgtgtaaa cagccagcat ggggttatat ctcggcgctg gatctgaaaa
    18001 ccaatgaagt ggtgtggaag aaacgtattg gtacgccgca agacagtatg ccgttcccga
    18061 tgcctgtacc ggtgccgttc aatatgggta tgccgatgct gggcgggcca atctccacgg
    18121 cgggtaacgt gctgtttatc gctgctacgg cagataacta cctgcgcgct tacaacatga
    18181 gcaacggtga aaaactgtgg cagggccgtt taccagcggg tggtcaggcg acgccaatga
    18241 cctatgaagt gaatggcaag cagtatgtgg tgatctccgc aggcggtcac ggttcatttg
    18301 gtacgaagat gggcgactat attgtggctt atgcgctgcc ggatgatgtg aaataaccat
    18361 aatgcccgga gagatccggg catatttccg aatacggtct ttttataccg taaaccctaa
    18421 catcatcccc gtatcttcat gctccagcag atggcagtgc gccatataag catgttcttt
    18481 cggtgcgttg tgattaaact tcaccagcac ttcactgaca ttgccttcta ccttaacggt
    18541 atctttccag cccgcgcgat gagccgctgg cggtttgcca ttttctgaca agatacggaa
    18601 ctgggtgccg tgaatatgga acggatgcag catcatgtcg cccacgccag agataaccca
    18661 acgttcgtac tgccctttcg ccgccgcaaa catcggctta ttcatatcaa acgcctgacc
    18721 gttgatttta ttggcatggt ggaaatcgaa cttcccgcca tggttcatat gattcatatt
    18781 gccgtgcccc atatggccca tcatctggct atgatccatc ccggccatcg cctgatcgcc
    18841 atatttctcc atcagcatct gcatccccat catatcgagc atcgggtcca tagaaagttg
    18901 cagcttgcgt accgtcagcc cttccagcga aggtaacgca ggcaggctac ttaatgtgtc
    18961 tggcaaagca ccggaggcac taatagcaat cggctgaatc cgcattaccg gatgcggctt
    19021 gtcaaacggc gcaatcgcca tccccatctg gctgaccggt agcgtcacca ggtcaaaggg
    19081 tttgttgtcg ttaacctcca ccagcacttc aaaacgctcg cccatcagca ccggcagctc
    19141 gctcaccttc accggttcag gtagcagacc accgtcgctg gcaatcacat acagcgggcg
    19201 attgtcgctg gtggcgaaat tgagcgagcg ggcattacag ccattaagca aacgcaggcg
    19261 cagccaacca cgcggggcag cgtgttgtgg gtagattgca ccgttggtca gcagcgtatc
    19321 gccaaaccag cccacggcgg cggtcatcac atccagttga taatcaatct gcccatcagc
    19381 gttaaatttt ttatcctgaa caatcaccgg aacatcatcg atgccccact gttttggcag
    19441 cattaatttc aggatctcgt catcttcaat taccaccagc ccagccaacc ccatcgccac
    19501 ctgtcgcccg gtcttgccat gttgatgtgg atggaaccag caagtagcgg caggttgatc
    19561 aacgttcaac gtcaccgagc gcttgccacc tggcagaata attccctgcg ggccgccgtc
    19621 gacttcaccc ggtacttcca gcccgtgcca gtgcaacgtc gtctcttccg tcagttggtt
    19681 atagatatca accgttaccg ctttgccgcg ctgtaatttc accgccggcc ccagcagatt
    19741 gccgttatag ccccaggtag ttgcagtttt ctcgccaaag gtagactgac ctgcgccaat
    19801 agttaactga atgcgattac gggcatcggt cgtgagcaaa tcagggatcg gtaacgttgg
    19861 gcgttccgcc gcaaagactg cgcggctcca cagcggcaag gctgaagcca cacccagcgc
    19921 gacggaatat tttaagaaat cacgacgttg catagttatt tccttattct taagcaggcg
    19981 aaacaaaatc aaacgttgag catagtcctt ccccttacgg gaaggtcaag ccgcagacac
    20041 aatttaatcg acaataatgg tcgtcgcctc gctcgcagtg tgctaacgtt tatcttcttt
    20101 aagcccctgg tagaagcaat gaagacgttt ttcagaacag tgttattcgg cagcctgatg
    20161 gccgtttgcg caaacagtta cgcgctcagc gagtctgaag ccgaagatat ggccgattta
    20221 acggcagttt ttgtctttct gaagaacgat tgtggttacc agaacttacc taacgggcaa
    20281 attcgtcgcg cactggtctt tttcgcccag caaaaccagt gggatctcag taattacgac
    20341 accttcgaca tgaaagccct cggtgaagac agctaccgcg atctcagcgg cattggcatt
    20401 cccgtcgcta aaaagtgcaa agccctggcc cgcgattcct taagcctgct tgcctacgtc
    20461 aaataatccc tgataccttt ttgtagaaat attgaccgtg catccgcggt caatgttagc
    20521 tattatgttg cgcccttttt ttacgggtgt taacaaagga ggtatcaacc catggccgaa
    20581 aaaaaacagt ggcatgaaac gctacacgac cagtttgggc agtactttgc ggtagataac
    20641 gttctgtatc atgaaaagac cgatcaccag gatctgatca tttttgagaa cgctgcattt
    20701 ggtcgcgtaa tggcgctgga tggcgtagta caaaccaccg agcgcgacga gtttatctat
    20761 catgagatga tgacccatgt tccgctactg gcccacggtc acgcgaaaca tgtgctgatt
    20821 atcggcggcg gcgacggtgc catgctgcgt gaagtaaccc gacataaaaa cgttgagtca
    20881 atcacgatgg tggaaatcga tgcgggtgtc gtgtcgttct gccgtcagta tctgcccaac
    20941 cataacgccg gtagctacga cgatccgcgc tttaagctgg tgatcgacga tggcgtcaat
    21001 ttcgttaatc aaaccagcca gacctttgat gtcattatct ccgactgcac cgatcctatc
    21061 ggtcccggcg aaagtctgtt cacctcagca ttttatgaag gctgcaaacg ttgcctgaat
    21121 cctggcggta tcttcgtcgc acaaaacggc gtctgctttt tacagcagga agaagccatc
    21181 gacagccatc gcaaactcag ccattacttc agcgacgttg gcttttatca ggcggcgatc
    21241 ccgacctatt acggcggtat catgactttt gcatgggcga cagataacga cgccttacgc
    21301 catctctcaa ccgaaattat tcaggcgcgt tttctcgctt ctggcctgaa atgccgttat
    21361 tacaatccgg caatccatac ggcagctttt gccttacctc agtatctgca agacgcactg
    21421 gcttcacagc cgtcctaagg agaagataag aaattgaaaa aactgaaact gcatggcttt
    21481 aataatctga ccaaaagtct gagtttttgt atttacgata tctgctacgc caaaactgcc
    21541 gaagagcgcg acggttatat tgcttatatc gatgaactct ataatgccaa ccgtctgacc
    21601 gaaatcctgt cagaaacctg ttccattatc ggggccaata ttcttaacat cgcccgccag
    21661 gattacgaac cacagggtgc cagcgtcact attctggtga gtgaagaacc ggttgacccg
    21721 aaactcatcg acaaaacaga acaccccggc ccactgccag aaacggtcgt tgcccatctc
    21781 gataaaagtc atatttgcgt acatacctac ccggaaagtc atcctgaagg cggtttatgt
    21841 accttccgcg ccgatattga agtctctacc tgcggcgtga tttctccgct gaaggcgctg
    21901 aattacctga tccaccagct tgagtccgat atcgtaacca ttgattatcg cgtgcgcggt
    21961 tttacccgcg acattaacgg tatgaagcac tttatcgacc atgagattaa ttcgattcag
    22021 aactttatgt ctgacgatat gaaggcgctg tatgacatgg tggatgtgaa cgtctatcag
    22081 gaaaatatct tccataccaa gatgttgctt aaagagttcg accttaagca ctacatgttc
    22141 cacaccaaac cggaagattt aaccgacagc gagcgccagg aaattaccgc tgcgctgtgg
    22201 aaagaaatgc gcgagattta ttacgggcgc aatatgccag ctgtttaacg gctctggcgg
    22261 agctcccagg ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca
    22321 cgacctgcag aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca
    22381 tcccctcttc catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt
    22441 caccgtccat ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt
    22501 tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc caggttttct ttcacctctt
    22561 cattaaacca gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg
    22621 taatatcgcg cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga
    22681 aatcgcattg cgccattgat gtaattatcg cagccttacc ggagaaaaac agcggaacgg
    22741 cgatggttta gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat
    22801 gcgcagcgcg tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt
    22861 cggtgtactg agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat
    22921 ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg
    22981 caacagccgc caattctgca gaagccagga agacattcgc gccagtcccc agacggttcg
    23041 ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac
    23101 ccatacacag ggaacagcca gggatctcga tacgcgcacc gctcttaccg aagacgctat
    23161 agtagccttc ttcggtcaac tgcgcggcat ccatacgggt tggcggtgcc acccacaggc
    23221 gcgtcggcaa ctggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga
    23281 tgttggtcat gcaggaaccg ataaacactt cgtcgatctt ctcgccctgt accgcagaca
    23341 gcggacgtgc gtcgtccggg tcgttcggtg cacagaggat tggctcttta atatccgcca
    23401 gatcgatgtc gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat
    23461 tcgccagcca tttttccatg ccctgaatac gacgttccag ggtacgacga tcgccgtaac
    23521 cttccgcgat catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt
    23581 ctttgttcag cttgatggta caaccagcgg cagaacgctc ggcggacgca tcggtcagtt
    23641 caaatgcctg ctcaactttc agatccggca gaccttcaat ttccagaatg cgaccagaga
    23701 agatgttttt cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg
    23761 ggatcgcgtg taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca
    23821 ccagaacgga ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca
    23881 gaccagaacc cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcaccaccgg
    23941 taccgacggt atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg
    24001 gacgcagcga cacaccacca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca
    24061 cgtcaactgg cttcggatac gccgcggtat ggcagaaaga ctgcatcacc aggtcagccg
    24121 agaagcccag gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct
    24181 gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc cggacgaata cctttcacac
    24241 cgcaggcacg acctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat
    24301 ctttcgcctg acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg
    24361 tcaggccacg cccgataatc agcgggatac ggccgccagc acgcacttca tcaatcagca
    24421 cgtcggtttt cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac
    24481 ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga
    24541 ttggcagtgc acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga
    24601 ggcacaaacc accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca
    24661 gaacggagtt cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg
    24721 ccagcgggaa acctttctgt tgcagagctt caatctgttt gatcgggcca acaacacctg
    24781 gctggtctgg ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga
    24841 tatccgggcg cgaccacgca tccggtgccg gagaaaggtc atcggtgtta gtttcgccag
    24901 tgactttgaa gacagtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt
    24961 cggcatccgc ccaggactgc ataacctgct tcgcatattc gttgcctgct ttcgctttct
    25021 cttctacgtc atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa
    25081 taggtgccag tttggcatca tccagcgcgt cgatcagcga atgaatgttg taaccaccct
    25141 gcatggtgcc cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc
    25201 ctttcgcgat agcagccagg aagcctgctt tgacataggc ggcttcatcg acgcctgggg
    25261 gaacacggtt ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca
    25321 gcagctctac aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag
    25381 cggcacgctc agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca
    25441 ttgtcatagt gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta
    25501 aatgcccagt accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac
    25561 aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc
    25621 cgtaaaaact tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta
    25681 gtcaaaattt aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt
    25741 cgccgaccat tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc
    25801 ccatgtgctg gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat
    25861 aaaaagccgc acagtggaag cgcctgtgga accgcaatcg acacaacaga ctgcgcctga
    25921 catcaccgca gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt
    25981 caaatccgca ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc
    26041 cgattctctg ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc
    26101 agtgcaatgg tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc
    26161 tgaccagccg tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt
    26221 gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca
    26281 ggacgtgatg gactcggtac agcgattgcg gcaactggcg caacaaaccg gttcgctgaa
    26341 gtcatcaacc gaacaaaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac
    26401 agtcacggca cccgtcatac catccaatac cgttttaact gccagccccg tcattacaga
    26461 gcctgcaaca accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa
    26521 ctacaaccca accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct
    26581 gccaccacca gccggagaac cgtttattga cagctttgtg cgcggattcg gctatagcat
    26641 gggtgttgct accacgtacg cactattcag cagcatcgac tgggatgacg acgatcatga
    26701 ccatcatcat catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa
    26761 tggctggcaa cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac
    26821 cggtgagcat cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg
    26881 tgtgccctat catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg
    26941 aatgagcgcc acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag
    27001 tcagtttcag caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc
    27061 agcggcacag cggtttaatg aagctgaaaa ctatgggagc tatgacgact tccgcgactt
    27121 cagccgtcgc caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc
    27181 ggcctcgcct gagcagcgcc aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca
    27241 acagcaacgt gataacgccc gccagcgtgt tcaatcagca tcacctgaac aacgtcaggt
    27301 ttttcgggaa aaagttcagg agagccgccc acaacgtcta aacgacagta accatactgt
    27361 caggctgaat aacgagcaac ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag
    27421 gcgacaggaa aggtaaattg cagacgtaaa aaaagcggcg tggttagccg cttttttaat
    27481 tgccggatgt tccggcaaac gaacaattac ttcttcttcg ctttcgggtt cggcaggtcg
    27541 gtaatgctac cttcgaacac ttctgccgcc aggcccacag actcgtgcag agtcgggtgc
    27601 gcgtggatgg tcagtgcgat gtcttcagca tcacaaccca tttcgattgc caggccgatt
    27661 tcacccagca gctcgccgcc gttggtaccg acaatcgcac caccgatcac acggtgagat
    27721 tctttgtcga aaatcagctt ggtcataccg tctgcgcagt cggaagcgat agcacgacca
    27781 gaagcagccc acgggaaggt ggcggtttca tagctgatgc ctttctcttt cgcttctttc
    27841 tcagtcagac ctacccatgc aacttctggt tcggtatagg cgatggacgg gataactttc
    27901 ggatcgaagt agtgtttctt accggcgata acttcagcgg caacgtgacc ttcgtgaaca
    27961 cctttgtgtg ccagcatcgg ctgaccgacg atatcgccga tagcaaagat gtgcggtacg
    28021 ttggtacgca gctgtttgtc aacgcggatg aaaccacggt cgtccacttc cacgccagct
    28081 ttgcctgcgt cgaggttttt accgttcggc acacgaccaa tcgctaccag cacggcgtcg
    28141 taacgctgcg gttcagcggg tgcttttttg ccttccatcg tcacataaat accgtcttct
    28201 ttcgcttcaa cggcggtaac tttggtttcc agcatcaggt tgaatttctt gctgatacgc
    28261 ttggtgaaga ctttaacgat gtctttgtca gctgccggga taacctggtc gaacatttca
    28321 accacgtcaa tctgtgaacc cagcgcgtgg tatacggtgc ccatttccag accgatgata
    28381 ccgccaccca ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag
    28441 tcccagatac gcggatcttc atgcggaata aacggcagtt ggatcgggcg agaacccgct
    28501 gcaatgatcg cattgtcgaa gttgatcacg gttttgccgt tctcaccttc aacttccagg
    28561 gtgttagccc cggtgaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc
    28621 ataccagcca gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg
    28681 tcaatatcgg ttttcggttc gccgaagacg ataccgtgtt cagccagcgc tttggcttct
    28741 tcgataactt ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa
    28801 acaccgccaa gggtgttgta acgttctacg attacggttt ccagacctaa atcagcgcaa
    28861 cggaaggcag cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt
    28921 tcagtactca tcatgacctc tatatattta tctccggcgg tcatacccgt cgtctttcag
    28981 gccgcagcgg cgttagcttc acgccctcac cccagtcact tacttatgta agctcctggg
    29041 gattctggcg cttgctacct tgctgcaccc agaaatccat agggtatgtg aagtaatacc
    29101 ctaaccacca ccgggtcgtt ctatccgtcg gtattttaca aaattgtta
//
LOCUS       DAZKGS010000048        29083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKGS010000048 DAZKGS010000000
VERSION     DAZKGS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..139
                     /locus_tag="ACHOQY_002028"
     CDS             <1..139
                     /locus_tag="ACHOQY_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9678135.1"
                     /translation="VDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPRSS
                     E"
     gene            complement(221..308)
                     /locus_tag="ACHOQY_002029"
     tRNA            complement(221..308)
                     /locus_tag="ACHOQY_002029"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Ser,seq:tga)
     gene            735..1853
                     /gene="hyaA"
                     /locus_tag="ACHOQY_002030"
     CDS             735..1853
                     /gene="hyaA"
                     /locus_tag="ACHOQY_002030"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9678136.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1850..3643
                     /gene="hyaB"
                     /locus_tag="ACHOQY_002031"
     CDS             1850..3643
                     /gene="hyaB"
                     /locus_tag="ACHOQY_002031"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9678137.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            3662..4369
                     /gene="hyaC"
                     /locus_tag="ACHOQY_002032"
     CDS             3662..4369
                     /gene="hyaC"
                     /locus_tag="ACHOQY_002032"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP9678138.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4366..4953
                     /gene="hyaD"
                     /locus_tag="ACHOQY_002033"
     CDS             4366..4953
                     /gene="hyaD"
                     /locus_tag="ACHOQY_002033"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9678139.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            4950..5348
                     /gene="hyaE"
                     /locus_tag="ACHOQY_002034"
     CDS             4950..5348
                     /gene="hyaE"
                     /locus_tag="ACHOQY_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9678140.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5345..6202
                     /gene="hyaF"
                     /locus_tag="ACHOQY_002035"
     CDS             5345..6202
                     /gene="hyaF"
                     /locus_tag="ACHOQY_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9678141.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6336..7881
                     /gene="appC"
                     /locus_tag="ACHOQY_002036"
                     /pseudo
     CDS             6336..7881
                     /gene="appC"
                     /locus_tag="ACHOQY_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            7893..9029
                     /gene="appB"
                     /locus_tag="ACHOQY_002037"
     CDS             7893..9029
                     /gene="appB"
                     /locus_tag="ACHOQY_002037"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9678142.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGIWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9042..9134
                     /gene="cbdX"
                     /locus_tag="ACHOQY_002038"
     CDS             9042..9134
                     /gene="cbdX"
                     /locus_tag="ACHOQY_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9678143.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9214..10512
                     /gene="appA"
                     /locus_tag="ACHOQY_002039"
     CDS             9214..10512
                     /gene="appA"
                     /locus_tag="ACHOQY_002039"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9678144.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10627..12807)
                     /gene="etk"
                     /locus_tag="ACHOQY_002040"
     CDS             complement(10627..12807)
                     /gene="etk"
                     /locus_tag="ACHOQY_002040"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9678145.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(12827..13273)
                     /gene="etp"
                     /locus_tag="ACHOQY_002041"
                     /gene_synonym="yccY"
     CDS             complement(12827..13273)
                     /gene="etp"
                     /locus_tag="ACHOQY_002041"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9678146.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13261..14400)
                     /gene="gfcE"
                     /locus_tag="ACHOQY_002042"
     CDS             complement(13261..14400)
                     /gene="gfcE"
                     /locus_tag="ACHOQY_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9678147.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14446..16542)
                     /gene="gfcD"
                     /locus_tag="ACHOQY_002043"
     CDS             complement(14446..16542)
                     /gene="gfcD"
                     /locus_tag="ACHOQY_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9678148.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(16542..17288)
                     /gene="gfcC"
                     /locus_tag="ACHOQY_002044"
     CDS             complement(16542..17288)
                     /gene="gfcC"
                     /locus_tag="ACHOQY_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9678149.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17285..17929)
                     /gene="gfcB"
                     /locus_tag="ACHOQY_002045"
     CDS             complement(17285..17929)
                     /gene="gfcB"
                     /locus_tag="ACHOQY_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9678150.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18036..18341)
                     /gene="gfcA"
                     /locus_tag="ACHOQY_002046"
     CDS             complement(18036..18341)
                     /gene="gfcA"
                     /locus_tag="ACHOQY_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFP9678151.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(18783..18995)
                     /gene="cspH"
                     /locus_tag="ACHOQY_002047"
     CDS             complement(18783..18995)
                     /gene="cspH"
                     /locus_tag="ACHOQY_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9678152.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19281..19493
                     /gene="cspG"
                     /locus_tag="ACHOQY_002048"
     CDS             19281..19493
                     /gene="cspG"
                     /locus_tag="ACHOQY_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9678153.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            19504..19692
                     /gene="ymcF"
                     /locus_tag="ACHOQY_002049"
     CDS             19504..19692
                     /gene="ymcF"
                     /locus_tag="ACHOQY_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9678154.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            19667..19897
                     /gene="ymcE"
                     /locus_tag="ACHOQY_002050"
     CDS             19667..19897
                     /gene="ymcE"
                     /locus_tag="ACHOQY_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9678155.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            19887..20060
                     /gene="gnsA"
                     /locus_tag="ACHOQY_002051"
     CDS             19887..20060
                     /gene="gnsA"
                     /locus_tag="ACHOQY_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9678156.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20108..21181)
                     /gene="yccM"
                     /locus_tag="ACHOQY_002052"
     CDS             complement(20108..21181)
                     /gene="yccM"
                     /locus_tag="ACHOQY_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9678157.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(21253..23995)
                     /gene="torS"
                     /locus_tag="ACHOQY_002053"
                     /pseudo
     CDS             complement(21253..23995)
                     /gene="torS"
                     /locus_tag="ACHOQY_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            complement(24090..25163)
                     /locus_tag="ACHOQY_002054"
     CDS             complement(24090..25163)
                     /locus_tag="ACHOQY_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9678158.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSDSGRKSLIDRIKGGDAITLHVWLDRYERILTERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKH
                     HDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDLMAARYRDSRGREWDKIEIDK"
     gene            complement(25141..25359)
                     /locus_tag="ACHOQY_002055"
     CDS             complement(25141..25359)
                     /locus_tag="ACHOQY_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9678159.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(25399..25566)
                     /locus_tag="ACHOQY_002056"
     CDS             complement(25399..25566)
                     /locus_tag="ACHOQY_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678160.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(25602..25901)
                     /locus_tag="ACHOQY_002057"
     CDS             complement(25602..25901)
                     /locus_tag="ACHOQY_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678161.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(26059..26337)
                     /locus_tag="ACHOQY_002058"
     CDS             complement(26059..26337)
                     /locus_tag="ACHOQY_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFP9678162.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(26337..26924)
                     /locus_tag="ACHOQY_002059"
     CDS             complement(26337..26924)
                     /locus_tag="ACHOQY_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9678163.1"
                     /translation="MTISTKKPLYAEVHNVPDDYEFTDEELNRIIAGDMFTPRQAAIM
                     AREIQKLRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQW
                     LQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYEDGEMWS
                     AMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            complement(26921..27538)
                     /locus_tag="ACHOQY_002060"
     CDS             complement(26921..27538)
                     /locus_tag="ACHOQY_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678164.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPIGEVSEKRLGLVMDGTVDLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEA
                     IENAIEYIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQ
                     VIELTMLVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
     gene            complement(27542..27730)
                     /locus_tag="ACHOQY_002061"
     CDS             complement(27542..27730)
                     /locus_tag="ACHOQY_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678165.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            complement(27732..28400)
                     /locus_tag="ACHOQY_002062"
     CDS             complement(27732..28400)
                     /locus_tag="ACHOQY_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9678166.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            complement(28877..28984)
                     /locus_tag="ACHOQY_002063"
                     /pseudo
     CDS             complement(28877..28984)
                     /locus_tag="ACHOQY_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28981..29083)
                     /locus_tag="ACHOQY_002064"
                     /pseudo
     CDS             complement(28981..29083)
                     /locus_tag="ACHOQY_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
BASE COUNT         6822 a         7509 c         7297 g         7455 t
ORIGIN      
        1 agtagatctg gagatcgcaa cggaggaaga aaactcgttg ctggaagcct ggaagaagta
       61 tcgggtgttg ctgaaccgtg ttgatacatc aactgcacct gatattgagt ggccggaaga
      121 accaagatca tccgaataaa aatataccaa ttggtatacc aagtttatac caagagccag
      181 aaaagcaaag gggctaccat ttggtaaccc cttgattatt tggcggaagc gcagagattc
      241 gaactctgga accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc
      301 acacttccgg aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac ccctaatttg
      361 tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg tgaaataaca gcagattgat
      421 catttcatca ccatgaattc cttctctttt actcgtttag caaccggcta aacatcccca
      481 ccgcccggcc aaaagaaaaa taggtccatt tttatcgcta aaagataaat ccacacagtt
      541 tgtattgttt tgtgcaaaag tttcactacg ctttattaac aatactttct ggcgacgtgt
      601 gccagtgcag aaggatgagc tttcgttttc agcatctcac gtgaagcgat ggtttgcctt
      661 gctacaggga cgtcgcttgc cgaccataag cgcccggtgt cctgccggtg tcgcaaggag
      721 aagagacgtg cgatatgaat aacgaggaaa cattttacca ggccatgcgg cgtcagggcg
      781 ttacccggcg cagctttctc aaatattgta gtctggctgc cacgtcgctg ggattaggcg
      841 cgggaatggc accaaagatt gcctgggcgc tggagaacaa accccgcatt ccggtggtat
      901 ggatccacgg tctggaatgc acctgctgta ccgaatcttt tatccgctcc gctcacccac
      961 tggcgaagga cgtcatcctt tccctgattt ccctcgatta tgacgatact ttgatggctg
     1021 ccgccggaac ccaggcggaa gaagtcttcg aagacatcat cacgcaatac aatggcaaat
     1081 atatcctcgc agtagaaggt aatccgccgc tgggcgagca ggggatgttc tgtatcagca
     1141 gcggtcgacc gtttattgag aaactcaaac gtgccgctgc cggagccagc gcgattatcg
     1201 cctggggaac ctgcgcgtcc tggggctgcg tgcaggccgc gcggcccaat ccgacgcagg
     1261 caacgcctat cgacaaagtc atcaccgaca aacccattat caaagtacct ggctgcccgc
     1321 cgatcccgga tgtgatgagc gccatcatta cttacatggt gacctttgat cgcttgccag
     1381 atgtcgacag aatgggccgt ccgctgatgt tctatggtca gcgaatccac gataaatgct
     1441 atcgccgcgc ccacttcgac gccggagagt tcgtccagag ttgggatgat gacgctgccc
     1501 gcaaaggtta ctgcctgtac aaaatgggct gcaaagggcc taccacctat aacgcctgtt
     1561 cttccacacg ctggaatgat ggcgtttctt tcccaatcca gtctggtcac ggctgcctgg
     1621 gctgtgcgga aaatggtttc tgggatcgcg gttcgttcta cagccgcgtg gtcgatattc
     1681 cgcaaatggg tactcattcc accgccgata ccgtcggttt aaccgcgctt ggcgtggtgg
     1741 cagcggctgt tggtgtgcac gcagtcgcca gcgccgttga ccagcgtaga cgtcataacc
     1801 agcaacctac agaaaccgaa catcagccag gcaatgagga taaacaggca tgagcactca
     1861 gtacgaaact cagggataca ccatcaataa tgccggacgc cgcctggtgg tcgacccgat
     1921 tacgcgcatc gaaggccaca tgcgctgcga agtgaatatt aacgatcaga atgtgatcac
     1981 caatgccgtc tcctgcggca ccatgtttcg cgggctggag atcatcctac aagggcgcga
     2041 cccgcgcgat gcgtgggcgt tcgttgaacg tatctgcggc gtctgtactg gcgtacacgc
     2101 cctggcttcg gtttacgcca tcgaagatgc tatcggtatt aaagtgccgg acaacgcaaa
     2161 tatcatccgc aacattatgc tggcaacgct ctggtgccac gatcatctgg tgcacttcta
     2221 tcagcttgcc gggatggact ggatcgatgt gttagatgcg ctgaaagccg acccgcggaa
     2281 aacctccgaa ctggcgcaaa gtctctcctc ttggccgaaa tcatcccctg gctatttctt
     2341 cgacgtacaa aaccgcctga aaaaatttgt tgaaggcggg cagttgggga tcttccgcaa
     2401 tggctactgg gggcacccgc agtacaaact gccgccagaa gctaacctga tgggctttgc
     2461 ccactatctc gaagctctcg atttccagcg tgaaattgtc aaaatccacg cggtctttgg
     2521 cggtaaaaac ccgcatccaa actggattgt cggcgggatg ccttgcgcca tcaacattga
     2581 cgaaagcggc gcggtcgggg cagtcaatat ggaacgcctg aacctggtgc agtcaattat
     2641 cacccgcacg gcggacttca ttaacaacgt gatgatcccc gacgccttag ccatcggtca
     2701 gttcaacaaa ccgtggagcg aaatcggcac tggtctttct gataaatgcg ttctcagcta
     2761 cggcgcattc ccggatattg ccaacgactt tggcgagaaa agtctgctga tgcctggcgg
     2821 cgcggtgatt aacggcgact tcaacaatgt gctgccagtg gatttggttg atccgcagca
     2881 ggtgcaggag tttgtcgacc acgcctggta tcgatatccc aacgatcagg tcgggcgtca
     2941 tccgttcgat ggcatcaccg acccgtggta caaccccggc gatgtcaaag gcagcgatac
     3001 caacattcag cagctgaatg aacaggaacg ctactcgtgg atcaaagcgc cacgctggcg
     3061 cggtaacgcg atggaagtgg ggccgctggc gcgcacgtta atcgcttatc acaaaggcga
     3121 tgctgcgacc gttgagtcgg tcgatcgcat gatgtcggcg ttgaacctgc cgctttccgg
     3181 tatccagtca acgttaggcc gcattttgtg ccgcgcgcac gaagcgcagt gggccgcagg
     3241 taagttgcag tatttcttcg acaagctgat gaccaacctg aaaaacggca atctcgccac
     3301 tgcttccacg gaaaaatggg aacctgcaac ctggccgaca gagtgccgtg gtgtcggttt
     3361 taccgaagcg ccgcgcgggg cgttaggcca ctgggccgcc attcgcgatg gcaagattga
     3421 tctctaccag tgcgtggtgc cgaccacctg gaacgccagc ccgcgcgatc ccaaagggca
     3481 gattggcgct tatgaagcgg cgctgatgaa caccaaaatg gcgatccccg agcaaccgct
     3541 ggagatcctg cgtactctgc acagctttga cccgtgcctc gcctgttcaa cacacgtgct
     3601 gggcgacgac ggtagcgagc tgatctctgt gcaggtgcgt taacagcgaa ggagaatcat
     3661 catgcaacag aaaagcgaca acgttgtcag ccactatgtc tttgaagcgc cagtccgcat
     3721 ctggcactgg ttgacggtgt tatgcatggc ggtgttgatg gtcaccgggt actttatcgg
     3781 caagccgcta ccttccgtca gcggcgaggc gacgtatctg ttctatatgg gctacatcag
     3841 gttaattcac ttcagcgccg ggatggtttt taccgtggtt ttgctgatgc ggatctactg
     3901 ggcttttgtt ggcaatcgat actcccgcga gctgtttatc gtgccggtat ggcgtaaaag
     3961 ctggtggcag ggcgtgtggt atgaaatccg ctggtatctg tttctggcaa aacgtccgag
     4021 tgccgatata ggccataatc ccatcgccca ggcggcgatg ttcggctatt tcctgatgtc
     4081 ggtctttatg atcatcactg gttttgcgct gtatagcgaa cacagccagt acgctatttt
     4141 tgcgccgttc cgttatgtgg tggaattttt ctactggatg ggtggcaact caatggacat
     4201 tcacagctgg catcggctgg ggatgtggct gattggcgcg tttgtgatcg gtcatgtcta
     4261 catggcgctg cgtgaagaca tcatgtccga cgacacggtg atctccacca tggtcaacgg
     4321 ctaccgtagc cacaaatttg gcaaaataag taacaaggag cgttcatgag cgagcaacgc
     4381 gtggtggtca tggggctggg caacctgctg tgggccgatg aaggcttcgg cgtgcgggtg
     4441 gcggaacggc tgtatgccca ttaccactgg cccgagtatg tggagattgt cgatggcggt
     4501 actcagggac tgaacttgct ggggtatgtc gaaagcgcca gccatctgtt gattctcgat
     4561 gccattgact acgggctgga acctggaacg ctgcgaacct atgccggaga acgcattccg
     4621 gcttatctca gcgcgaagaa aatgagcctg catcagaaca gtttctccga agtgttggcg
     4681 ctggcggata tccgcggaca tctgccagca catattgccc tcgtcggtct gcaacccgca
     4741 atgctcgacg actacggcgg tagcctgagc gaactggcac gggagcaact gcccgctgcg
     4801 gaacaggcgg cgctggcgca gctggctgcg tggggaattg tgccgcaacc ggctaatgaa
     4861 tcgcgctgtc tcaattatga ctgtctgtcg atggaaaatt acgaaggcgt tcgcttgcgc
     4921 cagtaccgga tgacacagga ggagcaggga tgagcaacga cacgccattt gatgcgttgt
     4981 ggcaacgaat gctggcgcgc ggctggacgc cagtcagtga atcccgtctt gacgactggc
     5041 ttacgcaagc gccagacggc gtgctgttat taagcagtga cccgaaacgc acgccagagg
     5101 tcagcgataa tccggtaatg attggcgaat tactgcgcga gtttcccgac tatacatggc
     5161 aggtggcgat tgctgacctt gagcagagcg aagccatcgg cgatcgcttt ggcgtctttc
     5221 gctttcctgc cactttagtg tttaccggcg gaaactatcg cggcgtgctg aatggtattc
     5281 acccgtgggc ggaactgata aacctgatgc gcgggcttgt cgaaccgcag caggagcgtg
     5341 cctcatgagc gaaacatttt tccatctgct ggggccagga acgcaaccga acgatgacag
     5401 tttcagcatg aatccactgc cgatcacctg tcaggtgaat gatgaaccga gtatggcggc
     5461 cctggagcaa tgtgctcaca gcccgcaggt gattgcgctg ttaaacgagt tacaacatca
     5521 actaagcgaa cgccaaccgc cgttgggcga ggtgctggca gtcgatctgt taaatctcaa
     5581 cgccgacgat cgtcacttta tcaatacgct tctcggggaa ggggaagtgt cagtgcgcat
     5641 tcagcaggct gacgacagtg aaagtgaaat acaggaggcg atcttctgcg gattatggcg
     5701 ggtgcgcaga cgtcgcggcg aaaagttgct ggaggacaaa ctggaggctg gctgcgcgcc
     5761 gctggcgttg tggcaggcgg caacgcaaaa tctcttgccg acagattcgc tgttaccgcc
     5821 gcccattgat ggcctgatga atggcctacc gttggcgcat gagttactgg cacatgtacg
     5881 taaccccgac gcgcagccgc acagcattaa tctgacgcaa ttacccatca gcgaggctga
     5941 tcggcttttt ctctcacgtc tctgtgggcc gggaaatatt cagattcgta ccattggcta
     6001 tggcgagagc tatatcaacg ccacggggtt acgccatgtc tggcatttac gctgtacgga
     6061 caccttaaaa ggcccgttac tggaaagtta tgaaatctgc ccaataccgg aagtggtgct
     6121 ggcagcgcca gaagatttgg tcgactctgc gcagcggctt agcgaggtat gtcagtggct
     6181 ggcggaagct gcaccgacgt aaaaagacgg taagtatcgc tttcagtctt atgaatatcg
     6241 caatcggcga atacctctgg tcgtagagtt tcaggataaa gagggagatc taccattatc
     6301 gggttatttt tctctcttcg cctacaggag tgcgcatgtg ggatgtcatt gatttatcgc
     6361 gctggcagtt tgctctgacc tgcgctgtat cactttttat ttgtacccct taccctgggg
     6421 ctgatttttt tgctggctat catggaaacc atttacgtgg tcaccggcaa aacaatctac
     6481 cgcgatatga cgcgcttctg gggtaagctc ttcggtatca attttgctct tggcgtggct
     6541 accggcctga ccatggagtt tcagtttggt actaactggt cattctattc caactatgtg
     6601 ggcgatattt ttggcgcacc actggcgatg gaagcattaa tggccttctt cctcgaatcc
     6661 acctttgtcg ggctgttctt cttcggctgg caacggctga ataaatacca gcacctgctg
     6721 gtgacgtggc tggtggcgtt cggttcaaat ctctctgcgt tgtggatatt gaatgccaac
     6781 ggttggatgc aatacccgac cggtgcgcat tttgatatcg acaccctgcg tatggagatg
     6841 accagcttca gcgagctggt ctttaacccg gtcagccagg tgaaatttgt gcacaccgta
     6901 atggcgggct acgtgaccgg ggccatgttt attatggcga tcagcgcctg gtatttactg
     6961 cgcggacggg agcgcgatgt cgcattacgt tcgtttgcca tcggttccgt cttcggtact
     7021 ctggcgatta tcggtaccct gcaactcgga gacagttctg cgtatgaagt cgcgcaagta
     7081 caaccggtaa aactggcggc gatggaaggg gagtggcaaa cggaacctgc acctgcaccg
     7141 ttccatgtgg ttgcctggcc ggaacaggat caagagcgta acgcctttgc catcaaaatt
     7201 cccgcgctgc tagggatcct cgccactcac tcattagata aacccgtgcc gggtctgaag
     7261 aatttgatgg ctgaaaccta cccacgcttg caacgcggac gtatggcctg gctgttaatg
     7321 caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc aggcattccg ggaactggaa
     7381 ggtgacctgg gctacggcat gttgctctcc cgctatgcgc cggatatgaa tcatgtcaca
     7441 gccgcacagt accaggcggc gatgcgtggc gcgatacctc aggttgcgcc ggtattctgg
     7501 agtttccgca tcatggtggg ctgtggttcc ctgctgctac tggtgatgct gattgcgctt
     7561 gtccagacgc tgcgtggcaa aatcgaccag catcgctggg tgctgaaaat ggcgctctgg
     7621 agtttgccgt tgccgtggat tgcgattgaa gccgggtggt ttatgaccga gtttggtcgt
     7681 cagccgtggg cgatacagga catcttaccg acatactccg cgcactccgc tttaaccaca
     7741 ggacaactgg ctttctcact gatcatgatc gtagggcttt acaccctgtt cttaatcgcc
     7801 gaagtctacc tgatgcagaa atatgcccgt ctggggccga gcgcgatgca gagtgaacaa
     7861 ccgacgcagc aacaggggta aaggagaaaa tcatgtttga ttatgaaaca ttgcgcttca
     7921 tctggtggct gctgattggc gtgatcctgg tggtctttat gatctccgac ggatttgaca
     7981 tggggatcgg ctgtctgctg ccgctggtgg cgcgtaatga tgatgaacgc cggatagtga
     8041 taaacagcgt tggtgcacac tgggaaggca accaggtctg gttgatcctc gctggtgggg
     8101 cattatttgc cgcctggccc agagtgtatg cagcggcgtt ttccggcttt tatgtggcga
     8161 tgatcctggt gctgtgctca ctgttcttcc gcccgctggc ctttgattat cgcggaaaaa
     8221 tcgccgatgc acgctggcgt aaaatgtggg acgccggtct ggtcatcggc agtctggtgc
     8281 cgccggtagt cttcggtatc gccttcggca acttgttgct cggcgtgccg tttgccttca
     8341 caccgcaatt acgcgtggag tatctcggca gcttctggca actgctgacg ccattccctt
     8401 tattgtgcgg attgctcagc cttgggatgg tgattttgca aggtggcatc tggttacaac
     8461 tgaaaactgt tggtgtgatt catctgcgtt cacagctggc caccaaacgc gctgcactgt
     8521 tggtgatgct gtgctttttg ctggcgggtt actggctgtg ggtcggtatt gatggctttg
     8581 tactgctcgc ccaggatgct aacggtcctt ccaatccgtt aatgaaactg gtggcagtgc
     8641 tacctagtgc ctggatgaat aatttcgtcg aatcgcccgt tttgtggatc ttcccgctgc
     8701 tggggttctt ctgcccattg ctgacggtga tggcgattta tcgtggtcgc ccgggttggg
     8761 gatttttaat ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc atcacgctgt
     8821 tcccctttgt catgccgtca agcgtgagtc caatctccag cctgacgttg tgggacagca
     8881 cttccagtca gctgacgctg agcattatgt tggtaatcgt gctgatattt ttgcccattg
     8941 tgttgctcta cactctctgg agctactaca aaatgtgggg acgcatgaca acagaaactc
     9001 tccgccgtaa cgaaaacgag ttgtactaag gagcaggaac aatgtggtat ttactttggt
     9061 tcgtcggcat tttgttgatg tgttcgctct ccacccttgt gttggtatgg ctggacccgc
     9121 gtctgaaaag ttaacgaacg taggcctgat gcggcgcatt agcatcgcat caggcaatca
     9181 ataatgtcag atatgaaaag cggaaacata tcgatgaaag cgatcttaat cccattttta
     9241 tctcttctga ttccgttaac cccgcaatct gcattcgctc agagtgagcc ggagctgaag
     9301 ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg ctccaaccaa ggccacgcaa
     9361 ctgatgcagg atgtcacccc agacgcatgg ccaacctggc cggtaaaact gggttggctg
     9421 acaccgcgcg gtggtgagct aatcgcctat ctcggacatt accaacgcca gcgtctggta
     9481 gccgacggat tgctggcgaa aaagggctgc ccgcagtctg gtcaggtcgc gattattgct
     9541 gatgtcgacg agcgtacccg taaaacaggc gaagccttcg ccgccgggct ggcacctgac
     9601 tgtgcaataa ccgtacatac ccaggcagat acgtccagtc ccgatccgtt atttaatcct
     9661 ctaaaaactg gcgtttgcca actggataat gcgaacgtga ctgacgcgat cctcagcagg
     9721 gcaggagggt caattgctga ctttaccggg catcggcaaa cggcgtttcg cgaactggaa
     9781 cgggtgctta attttccgca atcaaacttg tgccttaaac gtgagaaaca ggacgaaagc
     9841 tgttcattaa cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa tgtctcatta
     9901 accggtgcgg taagcctcgc atcaatgctg acggagatat ttctcctgca acaagcacag
     9961 ggaatgccgg agccggggtg gggaaggatc accgattcac accagtggaa caccttgcta
    10021 agtttgcata acgcgcaatt ttatttgcta caacgcacgc cagaggttgc ccgcagccgc
    10081 gccaccccgt tattagattt gatcaagaca gcgttgacgc cccatccacc gcaaaaacag
    10141 gcgtatggtg tgacattacc cacttcagtg ctgtttatcg ccggacacga tactaatctg
    10201 gcaaatctcg gcggcgcact ggagctcaac tggacgcttc ccggtcagcc ggataacacg
    10261 ccgccaggtg gtgaactggt gtttgaacgc tggcgtcggc taagcgataa cagccagtgg
    10321 attcaggttt cgctggtctt ccagacttta cagcagatgc gtgataaaac gccgctgtca
    10381 ttaaatacgc cgcccggaga ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg
    10441 cagggcatgt gttcgttggc aggttttacg caaatcgtga atgaagcacg cataccggcg
    10501 tgcagtttgt aatgcataaa aaagagcatt cagttacctg aatgctctga ggctgatgac
    10561 aaacgaagaa ctgtctaatg cgtagaccgg aaaaggcgtt cacgccgcat ccggccactt
    10621 tcagttttac tctttctcgg agtaactata accgtaatag ttatagccgt aactgtaagc
    10681 ggtgctggcg cgtttaatca caccattgag gatagcgcct ttaatattga cgcctgcctg
    10741 ttccagacgc tgcattgaca aactcacctc tttggcggtg ttcaagccaa aacgcgcaac
    10801 cagcaggctg gtgccaacag aacgccccac gaccgcggca tcactcaccg ccagcatcgg
    10861 cggcgtatcg acaatcacca gatcgtaatg gtcgttcgcc cattccagta attgacgcat
    10921 ccgatcgcgc atcagcagtt cagacgggtt aggtggcacc tgaccgcgag taatcacatc
    10981 aaagcctcct ttgccaaaat gctggatcac tttgttgagc tcatctttac ctgccagata
    11041 ttccgacaag ccatgttcat tactcacggt aaacaggtta tgcgaataac cacggcgtaa
    11101 gtcggcatca ataaataaca ctttttgatc ggactgggcg atcaccgctg ccagagttga
    11161 actgacaaac gttttaccac tgtctggcgt cgcaccggtg atcatcagaa tgttattctc
    11221 cgtctccatc atagcgaaat gcagactggt tcgtagcgca cgtacggctt ccacagcaga
    11281 atccgccggg ttatccaccg ccaggaaggg gatattttta gtacgatggc gctgctgatt
    11341 agaaaataaa tttttcttac gcagacgggt gcgtttatcc agccactcgg acattgggat
    11401 agtggcataa acgctgatgc cgtgctcttc cagttgttcc ggggcttcta caccacgacg
    11461 caacatcgca cgcgccagca cggcacccac agaaataaac aggccaagaa taaaaccaag
    11521 caccacattc aacgcttttt tcggtttcac tggctgcggc tgagtgactg ccgggtcgat
    11581 aatccgcacg ttaccaatgg cactggattt cgaaatactc aactcctgct ggcggttaag
    11641 taattgcaga tataccgcac ggcccgcttc tacgtcacga cttaaacgca acacttcctg
    11701 ttgggtggaa ggcattgccg atacccgctt attcaggcgt ttgcgttctt gctccagcgt
    11761 ctggcgtttt tccagcagcg cacgataagt tgggtgatct ttcttataca gctgggagat
    11821 ctctgcctcg cggaaggtca gctcattgag ttgattatca acgttcacaa tctgctcaag
    11881 aacggctttg gcttccaggt taaggtcaac cgaatcgcgc tgctggcgat aaacgttgag
    11941 tttttcttcc gcctggtcca gctcgctgcg cacttcaggt aactggcgct gtaagaattc
    12001 aaggctttgt gaatcctgcg ccgcctggcg agcgatattc tgttgcaaat agttgttagc
    12061 gatgctgttc agaatacgag taatcaactg gggatcatca ccagtcatgg taagttccag
    12121 catcccgctt tctttactgc gttcgctaac ggtaaaggtt tcctgcaatg cgttaatcgc
    12181 ttccagttcg gtacgctggc tcaggacaaa ctgtgttcct ggtttggcct taatgtccgc
    12241 gatagtcagc gcaacgccat ctttttccag acgctggccg accataccat tgacggtgaa
    12301 ctcttcacct tccagtgtat agtggccgtt ttccccaacc gtgagtgtca gttgctgatc
    12361 ctgaccattc agttgtggaa tatgcatcca gctgatcgcc agctcacctg gtttttcttt
    12421 ggttaatctc gcccagccgc gacccacaat cggaaaatac ttctgctcaa ctatgtcgcg
    12481 cagattcagt tcagcaatgg ttttaccgag aatcatgcgc gattgcagca gttggatctc
    12541 cggtgcagac tcgggcgatg agttagggat catatcgctc aggccgctga gaatggcgtt
    12601 gccctgtttt tgctcaacct ggaccagagt atctgcctga taaattggtg tgcttaacag
    12661 cgagtaagcg acagcgatca gcgtgaataa cgcggtcacg ctgataataa acttacggtg
    12721 atcccataac tcgccgacca gacgaagcag atcgatctca ttttcctgag tgctgcctgg
    12781 tggcgtattc atatttttag ttgtcatcgt attttataaa ctcaaattac cggctgaggc
    12841 gcttcgccca ttcctgactg gcgcgctcca acataccgta gacatgttca aatgcgtcct
    12901 gacttttacg atagggatcc gggatctctt tctgttccag ccattgccca aacagcattg
    12961 tcttcccgcg gacctcggga gcaattgcgg taacctgagc aatatgttcc gactccattg
    13021 ccagaatcag atcgtaattt cgtgccatct cagcagtgag cttacgtccg gcatgtcctt
    13081 caagagatac gccatgatta gcggcaacat ctgccgctgt cgcatctgca gggtgtttta
    13141 ccagaccatg aacacctgcc gatttgactt ttacgcccgg cagacgttta cgcagcaagc
    13201 gctcgccaat gggcgaacgg caaatgttgc cggtacaaac caccaggatt gagttaaatt
    13261 ttagttgggc catgtttgaa tccaccgtga agtctctgtc aggtcatgca ccccggaaat
    13321 cgtcggtacc agcagagaaa tcacacggtt ccaacgagcc agcggcgcgg tagtgacata
    13381 aacaatgtcg taaggttcaa gctggaattc cgttccgagt atcattgccg atgcgtcctt
    13441 cgcattcagc tgataaatat tggcgatttt gccattctgc ttgttttgcg ttgcacgaat
    13501 aacaaaaatc cccgtcgcat cagcgacatc ctgattcata ccctcagcgt tacccagcgc
    13561 ttccgccagg gtcataccgc tgcggtccat tttcagcgtg ctctgtttac ccacttcacc
    13621 catcacgaag actttcagat catcgttgcg cggaataaat aaaatatcgc ccgggtgcag
    13681 cagtttgttc tggcgcaagt cgccacgctg catcagcgca tagaggttca ctttggtctt
    13741 tacgccattc tgggtgagca ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc
    13801 ggcgttaatc gcatccatga tggtcagagg aatattggta atcggctgct gacccgattt
    13861 gctgacttcg ccggtgacgt acgctttttg cgagcggaac gccgcgacgc tgacatcgac
    13921 ttgcgggctt tcaatgactg aatccagacg cgctgtaatc tcgttgcgta cctgggtaag
    13981 tgttttcccg gccactttca ggcgaccaat ataggggtag aaaatcgcac catcggcgtt
    14041 aacccagttg ccggtatcac tggcgctacg gtattgccct gcgggggtgg ttagttccgg
    14101 gtgatcccag accgtaacca tcaacacatc cccaatgccg atacgatatt catagtttgc
    14161 gatttgctgc tccagctccg ggttagcctg cgacataatc ggtttggcgc gcaactgatc
    14221 aatcaatcct ggcgtgacgg gatagatatt gaccattttg tccaggtcat actgattgtc
    14281 aggtagttca atgacatcct tgttactggt cgataaattc tgaccaggaa caagggtaca
    14341 tgctgtcaaa gacagaacag ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat
    14401 tggtttatat ttatcagtca atgtaatgtt atgaattacg tcaggttatt gtccaaccgg
    14461 tacttctcgc tctgccgtca ttggatagag ctggtatttg cgtgacagca tttgtccacc
    14521 atcacgcgtc aatggtgtcc acgaaaccac cgcgcggttg cggttaggtc caatggtcat
    14581 caagtcgaat ggaatcgaga tataaaaacc tttactaaag ccgccttcgc cgtagtcatc
    14641 tttagatacg ttactgattg ccgcccatac ccctaccgcc acgccgctgt caaagcgttt
    14701 ggcgacgtcg atcgttgcgc ctttatcttt tgccagatat tgcccaacgc taagtttcat
    14761 cagtacgcca ttgagcgtcg gcgggttcca gtaagccgtc acgaaacctg ttggcgtgga
    14821 ataatcggtg aaacgcatca tgttgtccca gtcacgttgc ttcacgtagt taacgtccac
    14881 acccagcgcc cagctggcat ctagcgggcg ataaagcagc tcggaaccga caccggcgta
    14941 catcgtttcc agataaccgc catacacctg gccatagaaa ccattgccta agtcggcaaa
    15001 gtagttcgcc tgcaagttgt tgagataaac gtcattgcga acgtaatcac ggatatgcgt
    15061 gcgcacgcgg ggcagggtag agtccgcggg caacagcgaa gacttaaact tgtcgtagtt
    15121 gttgtaaata ttggtgaaaa taccgccatc aagcagcagg tggtcggtaa accagtagcg
    15181 ggcactggac atcagcccca gctggaacat atagaaatct tccgggccgc ccagcgactg
    15241 tgaaagtgtt gggttgaaag agtagctaaa gcgatcttca cgaatacggt agccccgacc
    15301 aaaggcagaa agatcttctg cttcaacacg ttgttgttga agttgctctg attgaccagg
    15361 cgctgtacca gccagctgtt tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc
    15421 catatgctcg cgcttttgcg tcacgctaat cttctcaacg ccttgcggca ggttattcac
    15481 cagaatccgg ttggcacgat cgaccgcttc gcgagaatca cggtatttgt attgttgacc
    15541 agacatatac agcgtcttat cgcgcagctg aatttccggt gcttcaaaac ctgcgttgta
    15601 cttcagcgcg gtaagttggt tcgctactgt ggtgtactgc aatccttcag attcaggcgc
    15661 aggttgatat gccggttttg gcgtatcgcg cagggcaggg cgcagatcgt tgaaattggt
    15721 ccgtaacgtg aagccaaaca tcaacgtgtt accgcgttca taactcaggt tgagatctgc
    15781 ccagctggca gcgcgataaa ctgcgccgac gttgaaatgg cttgcctgag gcagtttgcc
    15841 agcgaaatca ttctggtaat tgttgccatc gtattcgagt ttcagacgca ggggattcca
    15901 cggcgtttga tactcaatgc cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa
    15961 gctgatatcg cccgcatcgt gagactctgc gcgatgacaa tatttatcgc tcacccggca
    16021 aaacgggttg gtaatattgc ccgcattacc ggcgtagccc catgccatcc cgagggtgaa
    16081 atcaaatggc cccgcttgct tgctggccac cagatactca ccgtcaaaca gacccgtacc
    16141 agcaatatca cgtttaccaa acgccacttg cggtagccag taaccttctt cccacaggcg
    16201 aagtttaaaa tcgaatgatt tgtctttgta tgactgatcg ccgctgaaat cttcccactg
    16261 gctatatttg cgtgtgcgca catccgtata acgaatggtg ccttccagcc atgggaacag
    16321 cgccacggag gtggagtaga accggtattg atcgttatcc cggtagttga cgctgaattc
    16381 accttccggt gcgatgcgcg catttggcat ctgcaacaat cctgtgccgc caaagtctga
    16441 ttgcgacgaa cccagcggat ccgggtaggt taatacttca gcatgacagg cactggagac
    16501 ggcaatggcc aggcagctta aaagataaga attcttcttc atcagtcagg aacacgttgc
    16561 gttaaaacag acacaatctg gtcatttaaa tcagcatatt tttcgggcag tacatgcgca
    16621 gaaaagccca accaaagctg gctgccaggc ggtggttcca catgtctttt attccacagg
    16681 gcaacagggg cgacgacagt ctcaccctca ggcgtaatca ccataacgtt attcttgtcc
    16741 gcgcctgcaa ggcgggggtg atcttgcaga taatcggtaa cgctacggcc tgcctgccag
    16801 ggcaattgcc cggccccaga aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg
    16861 gtatagagcg tatagtcccc gaccaacggg ggattgctgt tctcatccac tcgtacaaaa
    16921 tcagggtcca gtttgaccgg taagcgacca gtgatattga gattgagtaa ctgctggcgc
    16981 acggatttaa tcgttgccgc cacatcgtcg tcagcttctg cttcccagga agcaagctgt
    17041 gccatgacat gttggtagtc tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag
    17101 gcaccaggcc accagaggcg atcgcgcagt tgcggttgtg tcaccaactg gacaacattt
    17161 tccatggggc caacggaaag tgtctgttgt tcgccaggca gataaatagt caccgttcct
    17221 tgcgcaaagg catggggtgt cattacgtaa agtacgctgg caatgaaata cgactgtaat
    17281 ttattcatgg tgcgggttta agaaacgtca tttctacggg aataacccct gcgcctaaca
    17341 tttgccgact ttgacgaacc tggccagtag ccgaatcgac ccagaatgtg ttttgccact
    17401 gtttttctgg ccgcgtggaa gtcacctctt cctgccagac attacacagc acattgcgac
    17461 ccgcaatatt cagcgtctct aatccagcaa aggaaaaagt agaactcagg gtcgcagagc
    17521 gaaaatggtt gtcttcactc cagagaatat cgcgcgtcca gcggctgcca tcttttattg
    17581 ccagcgcgtt acgcagaggg tcctgcccgg aatttgtcac ttccagcaga ttattattaa
    17641 gtttgacggt ttttaaaaga cgtccattgt gggtaaccag catggcgtta tcctgggata
    17701 accatttgct ttgttcttgt tctatatatc ccagtaccac aaagattcgt tgcccttcat
    17761 taaggcgtaa atacatcgtg gaataaggca acgcctggat ctgctgatcc gctacggtga
    17821 tatcctgatt atcgaaaagg ctggcgcgaa atgtatcgac catactttgc tggctgtgcg
    17881 tacatcccgc aagaaatagt gcgaaaatcg ataaaataag agggcgcacc tttaatcctt
    17941 gaaaggttaa ttaaaaaaat gcaaatctct aaagcccggt acgaatatcg caaccgggta
    18001 tcatttaact ttaatttcga cgcgttaaag cagtattagc gtgtagtgga cgtggtggtt
    18061 gaggtgttat gagaaccgcc atcgccactg tcatttgctg caacaactgc caacgtcgcc
    18121 acaccaacag ccgttgccac accgacaact gtcgccgtgc cgacagcgcc agcagccacg
    18181 ccaccagtgg tagtagcagt cgtggttgcg ccggtagtgg tcgttgtggt gccagtagta
    18241 cccgtcgttg cctcggtccc gtttgttgct tcaggggcag caaacgcagc tggcgtcgtc
    18301 aacatgaagg ccataaggat tgcagaaagc ttgtgtttca taacttttcc tttattcatc
    18361 gcatggacaa tacgtacgct tgagatcagg attgtctact ttgagtataa catccttgtg
    18421 aaatctccct ttcctcatac cgcttgcact atatactgcg ggtaggaaag gcgcgcagag
    18481 ggaaatataa gattgttaac tatccccctc tggaaatacg acttgtgagc ataagataaa
    18541 tcttagcaaa atacaaatta aagataagga aattgttacg aaagctatta attattgttg
    18601 gtaatatagt ttcaagtggt actatagctg gctgttaaat ggcatttata actattaggt
    18661 gcagagatat tcgcttaaat ggattagttt ctttgtggaa aagtaactgg tgttataatg
    18721 tatgatgttg actattgcac tattccaata aaaccagtca gctttaaaca agcagcgtca
    18781 tattaagaga gataaacatt tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat
    18841 tccacacgta atcctggtat aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg
    18901 acctgaactt ctttacgacc gtcggagggg ataatgaatc ctttgccgct tttgcgatca
    18961 aaggttttga caattcctgt cattttacgg gacaaacaaa ttccttactg aaaatactgc
    19021 gctgcactat acggggttaa taaaataaag ccagcgatat ttaagaccgc cggacggcta
    19081 aaataaaatt tgcttaatct caattatcat gcgttaatag ctgcgtcggt ttgaaagaca
    19141 gacagcatac aaagtagttt actaaagcag ttctcattat caggcattat ccccttcttt
    19201 tgagtctctc tcctgaacac taagtagttt ctgtattaaa gccctgtttg ccgaaaggcc
    19261 caaaatgaag gaagtaaaat atgtctaata aaatgactgg tttagtaaaa tggtttaacg
    19321 cagataaagg ttttggcttt atcactcctg atgatggcag caaagacgtt ttcgtccatt
    19381 tcactgccat ccagagcaat gaattccgca cgctgaacga aaatcagaaa gttgaatttt
    19441 ctattgagca ggggcaacgt ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc
    19501 gttattactc aacatctcca tttccgctgt ccatgttgtc atggttcaca gtaccgtaca
    19561 tcggcattcg atgtgacgga gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg
    19621 acgatgatca cctttgataa cgtcgcgctg caaatacgca cggaccatgc gccgctggat
    19681 ttcacaaaat aatatcaggc tccctcgtgg agcctttttt atatctgcct tatttttctt
    19741 caacgctgta tgtatagtaa gcgataacct gttgattatt gaatctttcg gggagatggc
    19801 ttataacatt tcttacctga ccagggtacc gggaaccaac accttactgg cgtgttgctg
    19861 tctttcaaga ccagaagagg ttaacagtga atattgaaga gttaaaaaaa caagccgaaa
    19921 cggaaatcgc cgactttatc gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag
    19981 aagtctctga aattcgcttc accgcacgag aaaaaatgac cgggcttgaa agttatgatg
    20041 tcaaaatcaa aataatgtga ttttgtgaac atcacccgtg cgaggtgatg ttccgcttgt
    20101 tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac aattcatccg
    20161 gtaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgca aacataatcc
    20221 ccagaaccag tagcgtcatt aaccagccag acaacgcaaa ggctttttta tttgccgcag
    20281 gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg caagtcatac
    20341 agccggtaca ttccactgtt cgtacctgaa ttaatttatc gaccgggatc cgtgatgggc
    20401 aattttttgc gcatttgcca cagtcgatac aactttcggc attgcgacga atcttaaacg
    20461 gcgacaatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa cgacaccagg
    20521 catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat gtcgcggtcc
    20581 ccatatgacg aaagaaatcg agcattttaa cgtccatcac cacgctgtag ggcgacaaca
    20641 taaaatagtg aatcgcctga gcgggcatca ataatgcgat atagagaaaa aaactcaaca
    20701 gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca cattgccgac
    20761 caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc gggcataacc
    20821 atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc aataacagca
    20881 tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc agattcatca
    20941 gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat gtcgtgctac
    21001 ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa atattcattg
    21061 ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag tcattccacg
    21121 gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt ttctctgcca
    21181 tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg ttcttaactg
    21241 gctgatattg attcaaatcg cgttcaggtc tttcttattc aaccatgctt ccagatcggc
    21301 aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct gctccagttg
    21361 tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt ttagctgatg
    21421 cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga tttcatcgag
    21481 aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct ccgtccccat
    21541 taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat tgacttgcag
    21601 ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt tagggataat
    21661 cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat gggcgctaaa
    21721 gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg taatgccgtc
    21781 gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat tttgcagtgt
    21841 ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg tgttcagcat
    21901 ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac gtaaaccgtc
    21961 aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac gtaacggcaa
    22021 gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt cgccgcccat
    22081 cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc gtttgccgct
    22141 tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac cgcagccgct
    22201 gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga taatgtaccc
    22261 ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac gaatacgtcg
    22321 tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca ggcgaatcgg
    22381 gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg ggcgcggttc
    22441 aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg aataatcgag
    22501 aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc gcaaatcatc
    22561 acgctgggcg ttaagtgcgg ggttatctgc cagcagttga gcagtgccga gaataccgta
    22621 cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg cctggctggc
    22681 tttttctgct tccgcccgtg cctgtcggtg ttctatcacc agttcctgca attcagctgt
    22741 acgcgctttg acctgcgccg ccagctgttc gcggtggcga ttcagtgcat gaacattgct
    22801 gcgaaacgca tccatcagcc gcccgatggt atccagctcc cgtacgccag cggtttccgg
    22861 gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt gttcggcaag
    22921 tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg cgcaaagaga
    22981 aactattccc agcaataaca ggctgtattg cccgcgtgca ctcgcttttt ccagatgcgc
    23041 cagtccgtgc tgattacgca gctcaatggt atcgaccagc tgactgactt cgctactaaa
    23101 ctgcgcgaac tgggcgatgt tattttgggc gagagtttgc aggtgagtgc tgatttcact
    23161 gtcctgctga tacagcgcca gtagatcgct atattggcta acggtagtta acgttgttgc
    23221 gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca gaattttcac
    23281 cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct ccagccccag
    23341 attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca tctggttaac
    23401 atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct gacgctgatc
    23461 ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg tcgcattatt
    23521 cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct gctgactgag
    23581 ttgccgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt gctgacgtaa
    23641 tgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac cttgttcccg
    23701 taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc gcccctgcgc
    23761 ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa gttcccaggc
    23821 gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct gagtgttgtc
    23881 tttttccacc tggctgaaag cgcaggttgt accatcccac cagggtactg gtcagggtta
    23941 acagcgccat cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg
    24001 gtgcacttaa ggtgaataag ttgtatattt aaaaactctt ttattatcaa tgaattaatg
    24061 taagtgggtc attattagtc aaaataaaat catttgtcga tttcaatttt gtcccattcc
    24121 cgtccacggc tgtccctata ccgcgccgcc attaaatctg atttatgccc gagaagacgt
    24181 tgagcaaact tatcgccaat ctggttccgg tatagcctcg ctgacaggct acgcagttca
    24241 tggaatgttg gcgggtttcc atcaaatgag agtccagatg catttctcgc ctttgtaaaa
    24301 tactttgata ctgttttcgg ggaaagcgga tcgtgatgct ttgatgcgat tatagtttca
    24361 ctgctgctgg cctccctgca tttctgtagt gtatcagcca atgagatatt gagcgcgtca
    24421 atcgttagcg ttagcggaat ggcgagttta gcccctgttt tactctgttc aatgtgaaga
    24481 tggttgtcgt ttatgtctga ccatttcatt ctgcacaaat cgccgactct ctgccctgta
    24541 acgacggcca aatccatcgc cagccttagc cagataggga gaggttcggc tgcatggtaa
    24601 atctcgacat actcattagc tgtcagcctt gagcgcctta cttctgactt tgctgtacgg
    24661 gttgctgtta ccggattcgt tgcaacatgc ccctcggcta ttgcttcacg aaaaacgtca
    24721 acaagggttg acctgattaa ttttgcggaa gctgctttac cttctgccac gtaggtgttt
    24781 agcattgctg ccacttcttt cgttgatatg tcagtgagcg gtttgtccgg caattttctt
    24841 cggattgccc tgattttgct ggcgtagtcg agtagagttt tcggcctgat ccctctttcg
    24901 gtgaggattc tttcatatcg gtcaagccac acatgaagag tgattgcgtc accgccttta
    24961 attctgtcta tcagtgattt gcgtccgctg tctgagagta actcaatatt ggcctgtatt
    25021 gcttcagtga ttgctatcct cctgtctcgg cctaatccaa actctttacc cgtccttggg
    25081 tccctgtagc agtaatatcc attgtttctt atataaaggt tagggggtaa atcccggcgc
    25141 tcatgacttc gccttcttcc catttctgat cctcttcaaa aggctacctg ttactggtcg
    25201 atttaagtca acctttaccg ctgattcgtg gaacagatac tctctgccat ccttcaccgg
    25261 aggagggaat atcctgcatt cgcgcaccca tcgacgaact gtttcaaggc ttcttgggcg
    25321 tcgctggcga gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca
    25381 cgcaataaaa aaccgccatc aggcggcttg gtgttctttc agttcttcaa ttcgaatatt
    25441 ggttacgtct gcatgtgcta tctgcgccca tatcatccag tggtcatagc agtcgttgat
    25501 gtcctctgct tcgataactc tgttgaatgg ttctccattc aattcacctg tgactcggaa
    25561 gtgcatttgt gatttcccca aaagatgctt gagtgcgctt cttattcgat tcgcacacct
    25621 ggtatttcgc cttttgaaat ggctaagtca taaatttgcg cagcactata cccatctcgc
    25681 atccatgaat ctaaggcgcg aatagcctcg ctacgctttt tatcttctct ttcatttttg
    25741 atatcaacga ggacatcaac gcaattaagg catatgtgga ttttgtcctt acattcgatc
    25801 atggcagctt taccatgatt tccgccacac agtgagcata aatcttcagg gtctggctgg
    25861 tatttctgta acgttaaagg gttgaatgtt gaacaggcca taatcatctc cataaaacaa
    25921 aactcgccgt agcgagttca gataaaagaa atccccgtca gtgcgaggat tgttattgtc
    25981 ttttcttcgt gcatttgtcg cactttcggc accatccaga taggcacatc cgtccgcaat
    26041 taacacatat aggccacatc atttttcctc ttttggttta tgaatctgaa tggtcatgcc
    26101 gctttgagtg gtgactacaa tgacataacc aggctgaaga ctgttaagat tgaatgcttc
    26161 gtaaaacgaa tccaaggcca gcgctttttt attctttcgg ttccaccaac gccatccctt
    26221 gctacaggct acactgacaa tccactgccc actcctgtaa gccatataaa accagatgag
    26281 caaaacctga aggaatgcta tccagtcaat aatcgtatat ttcgcgaagg agtccatcac
    26341 ttcacctcct gctgcggtgc tgctggcatt tcacgccagt gcgtaactga gtgcggatcc
    26401 ggatattcgg tgccatcatc ccagcgattg ccattccaca ttgcagacca catctcaccg
    26461 tcttcataca tgaccattac cggaattaac ttatccggca ttcgctcact acagcatatc
    26521 caaccatccg gagttaccgg agagttgccc gatagcgcat tacgcaatcg ctccagcttc
    26581 acgtattcct gaaccctgtt tccgtcgcat gcctgaagcc attgcgcagc cttttgcgca
    26641 tcagtgtgaa aggcacaagt gcgaccgtca tcaaattgca tttcgtagag gtcagcaact
    26701 tgttcaaact gcgtatgttg tgacttgtaa gtttggctta caggttcggc accatgaagc
    26761 atggcggcgc ggagtttctg tatctcccgt gccattattg cggcctgacg aggagtgaac
    26821 atatcacctg caataattct gtttagttcc tcgtcagtga actcataatc atcaggaaca
    26881 ttgtgaacct cagcataaag aggctttttt gtagaaatgg tcatcgtaaa acatcctccg
    26941 cacttaccag tccgtttcgc aaaagataat ccatcgccct atcaggtaat ttgcaatcag
    27001 gttttgcttt tttcagttgg ctgaccaatt gtttaaccag cattgttaat tcgatcacct
    27061 ggtagcgcgg caatggtgaa ttatcggatt tgccctgact gtcatcactg catgaatgcc
    27121 cttccagcca ggccaatgct tgtcgcatga aatacgcaat atgtttgccg tggtaatcgt
    27181 cttcatcgat gtgaaaagcg atactgcgga tgtattctat tgcattttca atggcctcca
    27241 acgctatcgg cgctggcggt gcggtataaa acttcgtccc cagcggcaac aacttcatcg
    27301 ctttttctcc cttaatgatg cgataagttg atttcccgcc aaggtctacc gttccatcca
    27361 taacaaggcc gagtcgcttc tctgaaacct caccaatagg ctctgcttcc agcgatgcca
    27421 gagcaattcg tgccagttct tccgcttctt ctgctggcag tacaacgttg ctaccaggtc
    27481 cgtatgtttc gcgccactgc ttgattgtca gtagtcgctc tttggtaata gtggtcatgg
    27541 gttatcctct tagcgccaca acgcgcactt ctgggtcaaa agatggtgca ttctcatctg
    27601 taacatcagc aacatgacct acaaagtcgt tatactcacc ctctgattgg tcatagccct
    27661 gccagaccac gacagcctca ggaggcattt ttctgagctt gctgattaac tgcctaaccg
    27721 tcagcgacat ctcactctcc tttgatgcga atgccagtag cgcggataga tttccattgt
    27781 ttccacattc ggttgaagtc tgtgcctgat aaggttgcgt tacggtatcc agtttcggta
    27841 cgcaatcctt caagcgtcga tttggagata caaattgaat gcattaccca tgattcaaac
    27901 aaatcacgct cccgggcttc gcaactttgc ttactcatct cttcgatacg ttcagccatc
    27961 gcagcacact cttcaaagtt gcttaatgct tttcgctccc attcggcgca ttgttttcca
    28021 agctctgcaa tcagcttgtc tttgccttcc agctcaacac gcagtttccc taccgttagc
    28081 gcaatatcct cgttctcctg atcgcggcgt ttgatgtatt gctggtttct ttccagttca
    28141 tccagtaatg ccagcacagt agccggattg gctgcggcga tgaattcagc attggcctgc
    28201 tgttccattt ggaaatcttc atcgaaaccg ctttctggat gtgctccttc aattctgcaa
    28261 atgggaagat atccaacaac ttcacgatga attaacgcat catcacaatc aaatcggctc
    28321 tctccatatt cgagcgacca tacaccacac gttgcttttt ctgccttggc acgcagtgcc
    28381 tgatagtcaa tcaggctcac tggttgcctc ctttgcgaat ctgttccgcc cattcttcaa
    28441 gggatttctc cgcatattca ccagacaggc catcaatcgg atgcggttca ttagccaact
    28501 cttctttcgc tgacagaatc atgcgcgtaa catcgaaaac ttcacgtaaa gacttattga
    28561 taaatccgtg gttgaaagca gcagcaagac ggcttgcggc atagttaata ccctcgttgc
    28621 gtgcttccgc acgtacttca gccaggaaag catcggtggc tggggttttg atttcgttaa
    28681 gcgcatcact gaataaacca cgctccatac ctagctctgc ttcgtaatcg gcatcgaatg
    28741 cagcgtcttt gcagaacttc ttcatccccg cactctccgc tgccagcgcc gcgcacttgg
    28801 cctcaaggtt atcaatcgtg attccagcag aacgacactc ccgcaacgcc gtttccagtt
    28861 ttgattcaag ttcaccgaac ttacggacaa gatattcagc gtttgtttcg ttaaccttta
    28921 gatctcgtgg gatgcattta cctttcagaa aaccatccat ctcaattagt gacatttgtt
    28981 tcattttttc ccactccgcc acatagcatt cagatatttg ttgtcattaa cagaaccgaa
    29041 actctttctc ttaagcaatt actctctcga tggcattggc ttt
//
LOCUS       DAZKGS010000049        29007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKGS010000049 DAZKGS010000000
VERSION     DAZKGS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..1726)
                     /gene="glnS"
                     /locus_tag="ACHOQY_002065"
     CDS             complement(62..1726)
                     /gene="glnS"
                     /locus_tag="ACHOQY_002065"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9678167.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            1837..1959
                     /locus_tag="ACHOQY_002066"
     CDS             1837..1959
                     /locus_tag="ACHOQY_002066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678168.1"
                     /translation="MIDEKKSGREDLHRLSLLDATLKRRIRHKADYFLISYSGV"
     gene            complement(1929..3875)
                     /gene="nagE"
                     /locus_tag="ACHOQY_002067"
     CDS             complement(1929..3875)
                     /gene="nagE"
                     /locus_tag="ACHOQY_002067"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9678169.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4208..5008
                     /gene="nagB"
                     /locus_tag="ACHOQY_002068"
     CDS             4208..5008
                     /gene="nagB"
                     /locus_tag="ACHOQY_002068"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9678170.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5068..6216
                     /gene="nagA"
                     /locus_tag="ACHOQY_002069"
     CDS             5068..6216
                     /gene="nagA"
                     /locus_tag="ACHOQY_002069"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9678171.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6225..7445
                     /gene="nagC"
                     /locus_tag="ACHOQY_002070"
                     /gene_synonym="nagR"
     CDS             6225..7445
                     /gene="nagC"
                     /locus_tag="ACHOQY_002070"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9678172.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7493..8245
                     /gene="nagD"
                     /locus_tag="ACHOQY_002071"
     CDS             7493..8245
                     /gene="nagD"
                     /locus_tag="ACHOQY_002071"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9678173.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8502..10166
                     /gene="asnB"
                     /locus_tag="ACHOQY_002072"
     CDS             8502..10166
                     /gene="asnB"
                     /locus_tag="ACHOQY_002072"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9678174.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10547..10623
                     /locus_tag="ACHOQY_002073"
     tRNA            10547..10623
                     /locus_tag="ACHOQY_002073"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10581..10583,aa:Met,seq:cat)
     gene            10632..10716
                     /locus_tag="ACHOQY_002074"
     tRNA            10632..10716
                     /locus_tag="ACHOQY_002074"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10666..10668,aa:Leu,seq:tag)
     gene            10740..10814
                     /locus_tag="ACHOQY_002075"
     tRNA            10740..10814
                     /locus_tag="ACHOQY_002075"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10772..10774,aa:Gln,seq:ttg)
     gene            10849..10923
                     /locus_tag="ACHOQY_002076"
     tRNA            10849..10923
                     /locus_tag="ACHOQY_002076"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10881..10883,aa:Gln,seq:ttg)
     gene            10939..11015
                     /locus_tag="ACHOQY_002077"
     tRNA            10939..11015
                     /locus_tag="ACHOQY_002077"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10973..10975,aa:Met,seq:cat)
     gene            11064..11138
                     /locus_tag="ACHOQY_002078"
     tRNA            11064..11138
                     /locus_tag="ACHOQY_002078"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11096..11098,aa:Gln,seq:ctg)
     gene            11176..11250
                     /locus_tag="ACHOQY_002079"
     tRNA            11176..11250
                     /locus_tag="ACHOQY_002079"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11208..11210,aa:Gln,seq:ctg)
     gene            complement(11404..12579)
                     /gene="ubiF"
                     /locus_tag="ACHOQY_002080"
     CDS             complement(11404..12579)
                     /gene="ubiF"
                     /locus_tag="ACHOQY_002080"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9678175.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12725..14149
                     /gene="miaB"
                     /locus_tag="ACHOQY_002081"
     CDS             12725..14149
                     /gene="miaB"
                     /locus_tag="ACHOQY_002081"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9678176.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14302..15342
                     /locus_tag="ACHOQY_002082"
     CDS             14302..15342
                     /locus_tag="ACHOQY_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9678177.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15339..15806
                     /gene="ybeY"
                     /locus_tag="ACHOQY_002083"
     CDS             15339..15806
                     /gene="ybeY"
                     /locus_tag="ACHOQY_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9678178.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15896..16774
                     /gene="corC"
                     /locus_tag="ACHOQY_002084"
     CDS             15896..16774
                     /gene="corC"
                     /locus_tag="ACHOQY_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9678179.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16799..18337
                     /gene="lnt"
                     /locus_tag="ACHOQY_002085"
     CDS             16799..18337
                     /gene="lnt"
                     /locus_tag="ACHOQY_002085"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9678180.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18415..20292)
                     /locus_tag="ACHOQY_002086"
     CDS             complement(18415..20292)
                     /locus_tag="ACHOQY_002086"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9678181.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20747..21655
                     /gene="gltI"
                     /locus_tag="ACHOQY_002087"
     CDS             20747..21655
                     /gene="gltI"
                     /locus_tag="ACHOQY_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9678182.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21825..22565
                     /gene="gltJ"
                     /locus_tag="ACHOQY_002088"
     CDS             21825..22565
                     /gene="gltJ"
                     /locus_tag="ACHOQY_002088"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9678183.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22565..23239
                     /gene="gltK"
                     /locus_tag="ACHOQY_002089"
     CDS             22565..23239
                     /gene="gltK"
                     /locus_tag="ACHOQY_002089"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9678184.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23239..23964
                     /gene="gltL"
                     /locus_tag="ACHOQY_002090"
     CDS             23239..23964
                     /gene="gltL"
                     /locus_tag="ACHOQY_002090"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9678185.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24082..25017
                     /gene="rihA"
                     /locus_tag="ACHOQY_002091"
     CDS             24082..25017
                     /gene="rihA"
                     /locus_tag="ACHOQY_002091"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9678186.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25101..26771
                     /gene="hscC"
                     /locus_tag="ACHOQY_002092"
     CDS             25101..26771
                     /gene="hscC"
                     /locus_tag="ACHOQY_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP9678187.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26831..28282)
                     /gene="djlC"
                     /locus_tag="ACHOQY_002093"
     CDS             complement(26831..28282)
                     /gene="djlC"
                     /locus_tag="ACHOQY_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFP9678188.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28279..28986)
                     /locus_tag="ACHOQY_002094"
     CDS             complement(28279..28986)
                     /locus_tag="ACHOQY_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9678189.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7119 a         7544 c         7330 g         7014 t
ORIGIN      
        1 tgtttgctat ttcttgccgg atgcgacgta aatgcctcac ccggcctggt agaacttaaa
       61 attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt
      121 tttccgctgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca aactggaatg
      181 ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg
      241 attccgggtt aatcaccgac aggaaatcat ccgcagcacc agggttcggc acgctgaaca
      301 gacggtcgta cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa
      361 cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac
      421 agaagatggt ggtgatattg ccttcggcat ctttctccac gcgttcagcc ttaatgacgt
      481 aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt
      541 cttcgcggaa atcggcgcga tcgatccaaa tctcaccgct aaacggcacc tgacgactcc
      601 ccatttccgg tttgttcgga tggttcggca tggtgaccat ttcgccttcg ccctgatagt
      661 tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga
      721 gatcttcacg gatgcaggat tccagcgatg ccatctcaat ggtgttgtcc tgcttggtca
      781 cgccgatgcg tttgcagaac tcacgaatag aagcagcagt gtaaccacga cgacgcagac
      841 cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca
      901 ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact
      961 ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt
     1021 cctggaactc aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt
     1081 gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa
     1141 atttaatacg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt
     1201 tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg
     1261 ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg
     1321 tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat
     1381 tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt
     1441 taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgactca acatactcga
     1501 tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct
     1561 gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg
     1621 gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga
     1681 tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa
     1741 agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag
     1801 ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa aaaaagcggc
     1861 agagaagatc tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat
     1921 aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga
     1981 gctttaatga tcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc
     2041 gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt
     2101 acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg
     2161 atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg
     2221 gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt
     2281 ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc
     2341 agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt
     2401 acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc tgacgcagcg
     2461 gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg
     2521 ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc acccagacgt
     2581 ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag acgggtgata
     2641 caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc
     2701 agttgattga ggccttcttc agtgttgctg ttggcttctt cagtaacgat ctcgtcttct
     2761 ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg
     2821 aagtagatag cgaagaagac aacacccata accagcagca tccagacgtt ctggctggcg
     2881 gccggcaggt tatacatcaa cgcgtagtcg atagcccccg ctgagaaaga gaagccagca
     2941 tggataccca gcagtgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg
     3001 tacagcagcg gtgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg
     3061 aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt
     3121 gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcataat cgggaagaag
     3181 ccggacatga acatccccgc ggtgccgtca ccggcataga agcggttgat atcaccgtgg
     3241 aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc
     3301 acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc
     3361 agcgcgcccg cagaaacgat ccactcgcct cctgcatgga tagcgtgctg taccggcggc
     3421 cagacgtaac caaaaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca
     3481 aagcgtttgc cgccgaagaa actcaggaag tccggcagtt taatatcgga ccaacggtta
     3541 taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct
     3601 gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagcgcc
     3661 gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg
     3721 aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc
     3781 tgaccgaatc gcagcaacag tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc
     3841 ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat
     3901 ttggctcgtt taaagccgta tttttatttt gctgcaaatt gtactgccga tgttctgtaa
     3961 tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa
     4021 acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc
     4081 taaagcagaa aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc
     4141 aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag
     4201 gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg
     4261 ccatatcgtc aatcgtatca atgcgttcaa accgactgcc gatcgtccgt ttgtactggg
     4321 cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc
     4381 aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc
     4441 gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga
     4501 tattccagca gaaaacatca accttctcaa cggcaatgcc ccggatatcg acgccgagtg
     4561 ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt
     4621 aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg
     4681 tatcaaaacc ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt
     4741 caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acattgctgg atgccgaaga
     4801 agtgatgatt ctggtgctgg gtagccagaa agcactggca ctgcaggctg cagttgaagg
     4861 ttgcgtgaac cacatgtgga ccatcagctg tctgcaactg catccaaaag cgatcatggt
     4921 gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt
     4981 agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggctcc ttgctcaggg
     5041 caatattttt taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc
     5101 ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc
     5161 tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc
     5221 tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc
     5281 gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc
     5341 tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg
     5401 cgcgttatgc gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa
     5461 ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct
     5521 gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg
     5581 gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt
     5641 tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt
     5701 acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg
     5761 gcgggcgcga tcctcgacga agctgacatt tattgcggta ttattgctga tggcctgcat
     5821 gttgattacg ccaacattcg taacgctaaa cgtctgaaag gcgacaaact gtgtctggtt
     5881 accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca
     5941 atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta
     6001 accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg
     6061 ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca
     6121 ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa aatcaccaag
     6181 accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac
     6241 aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc
     6301 tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc
     6361 ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg
     6421 atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt
     6481 tccacgcaat cggcgtacgg cttggtcgtc acgacgccac catcaccctg tttgatctca
     6541 gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag caaacgctgg
     6601 aacatgccct gttgaatgcc attgctcagt ttattgatag ctaccagcgc aaactgcgcg
     6661 agctgatcgc gatttcggtg atcctgccag ggcttgttga cccggacagc ggcaaaattc
     6721 attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg gaagaacgtt
     6781 ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact
     6841 tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg
     6901 ccgggattat ctctaacggg cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg
     6961 gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg
     7021 aaactatcgc tgccaacgcc gctattgaac aacgggtgtt gaatctgtta aagcagggct
     7081 accagagccg cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca
     7141 aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca
     7201 tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgccggt gaaatcaccg
     7261 aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccag gcgctgaagg
     7321 cgtttcgcac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg
     7381 cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa
     7441 attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat
     7501 taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg
     7561 tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa
     7621 ctatccttcg cagaccgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt
     7681 gccggatagc gtgttttata cctctgcgat ggcgactgcc gatttcctgc gtcgccagga
     7741 aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg
     7801 tttcactatt accgatgtga accctgattt tgtgattgtt ggcgaaacgc gttcctacaa
     7861 ctgggacatg atgcataaag cggcctattt cgtcgctaac ggtgcacgct ttatcgccac
     7921 caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat
     7981 tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt ggatcatccg
     8041 cgcggcatta aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg gcgataactt
     8101 gcgcaccgac attctggcgg gcttccaggc gggtctggag acgattctgg tgctttctgg
     8161 tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga tttacccgtc
     8221 ggtcgctgaa atcgacgtta tctgaatagc aaccacgtct cagggcgtgg tttcttattt
     8281 ttacccatca ttaaaatcat cccattcaat aaaactcgcc aaaaattatc gatccatcaa
     8341 taaatacatc accacaccct cccctttttc acaaatcagc aatcaccatt acgtttttta
     8401 tttttgcact gccaaatcgc ttgcgcacgg tgctgctttg cggcattttt ttaaacaagc
     8461 aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca atttttggcg
     8521 tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc
     8581 tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac gccattctcg
     8641 cccacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac
     8701 aaaaaactca tgtgctggcg gtaaacggtg aaatctacaa ccaccaggca ctgcgcgccg
     8761 aatatggcga tcgttatcag ttccagaccg gatctgactg cgaagtgatc ctcgcgctgt
     8821 atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt
     8881 acgacagcga aaaagatgca tatctgattg gtcgcgacca tctggggatc atcccactgt
     8941 atatgggcta tgacgagcac ggtcagctgt atgtggcctc agaaatgaaa gccctggtgc
     9001 cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagt caggacggcg
     9061 aaatccgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa gataacgtga
     9121 ccgacaaaaa cgagctgcgt caggcactgg aagattccgt taaaagccat ctgatgtctg
     9181 acgttcctta cggtgtgctg ctctcgggtg gtctggattc ctcaattatt tccgcgatca
     9241 ccaagaaata cgccgcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc
     9301 aattacactc ctttgctgta ggtctgccgg gttcaccgga tctgaaagca gcccaggaag
     9361 tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg
     9421 atgccatccg cgacgtgatt taccacatcg aaacctatga tgtgacaact attcgcgctt
     9481 caacaccgat gtatttaatg tcgcgtaaga tcaaggcaat gggcattaaa atggtgctgt
     9541 ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccacaaa gcaccgaatg
     9601 ccaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg
     9661 cgcgcgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca
     9721 aaaaattcct tgatgtggcg atgcgcatta acccgcagga taaaatgtgc ggtaacggca
     9781 aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct
     9841 ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag
     9901 aagtggcggc gcagcaggtt tctgatcagc aactggaaac tgcccgcttc cgcttcccgt
     9961 acaacacgcc aacctcaaaa gaagcgtatc tgtaccggga gatctttgaa gaactgttcc
    10021 cgcttccgag cgccgctgag tgcgtgcctg gcggtccttc cgtcgcgtgt tcttccgcta
    10081 aagcgattga gtgggatgag gcgttcaaga aaatggacga tccgtctggt cgtgcggttg
    10141 gcgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa atccggggct
    10201 gtttcagtta ttgaggtgct tgttaattct gatatgcagt aaggaaatgt tccgggtttg
    10261 ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt aatttcccac
    10321 cactattgtt gaattccgaa tttcatcctg tacgacaaaa gaatggctgt agggtgggga
    10381 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca
    10441 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
    10501 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct acgtagctca
    10561 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    10621 ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
    10681 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
    10741 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    10801 aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc
    10861 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    10921 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    10981 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatacgttc
    11041 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    11101 tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga cgcttacatc
    11161 gtaagtgcgc ccagttgggg tatcgccaag cggtaaggca ccggattctg attccggcat
    11221 tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg gcgagctttt
    11281 tgcttttctg cgttcattca atgtcgaatg cgatgttgac acgtcttatc cttcaatgtc
    11341 ggatgcgacg ctgccgcgtc ttatccgacc tacggttggc acgcatccgg caatgttgta
    11401 aggctacaac cctaacgcat atttcagcgc ctgacgtttc aacacgccag cacgctctgc
    11461 cgccattaac ccgagattac gcacaaaacg cagcggcggc agattattac tgaatccggc
    11521 ataaaacaga tccataccac tttgcataat gaagttatcc gccatgcgcc gcatctggta
    11581 acgcttgagg acaggataac tggcccacgc ttcaccgtag ctgcgggcat tcaccagaac
    11641 atcaatcagg gcatcgacat cacgataacc aagattcccc ccctgccccg ccagcggatg
    11701 gatggtatgc gcggcatcgc ccaccagcgc aagccccggc tgcacatact gcaacgcatg
    11761 tcggcgcgtc agcggaaacg caccagcggc aagcggtgta acgtaaccca gacgcgacgg
    11821 gaaatgcttc gcgatttccg cctggagttg cgccatattc atattctgca actggcgaat
    11881 acgtaccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg gcagaaacgc
    11941 acgcggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt tctcacactg
    12001 tacgctaatc aacatacacg actgcgcata ctgccaggca tgaacgccaa ttcccgccat
    12061 ctgccgcacc tgcgaatttg cgccgtcagc accaatcacc agcttcgcgc gaatcacttc
    12121 accgcccttc agttccagct cctgaagatc attatctcga tgtaatgcaa tcagcgagcc
    12181 tggcacacgt aacgttactt tcggatgcgc ttccagcgcc tgccacaatg cctgttgcag
    12241 gacggtgttt tccaccatat agccaagcag cggcagctta agttcagcgg cgtcaaacat
    12301 cacatgcgcc gtttcccact cccacgtttc cagtctgcgg taaggatggc aacgcatagc
    12361 ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgatgcgg cgctgatcgc
    12421 cgagatccgc acgtccggtt ggctatcagc gacaaacggt gcaggttctg cgtgctcgat
    12481 caccgttacc gaaaatccgt gctgtgccag ccccagcgcc agtgcgccgc cgaccattcc
    12541 tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc ctgttagcaa
    12601 tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat actggtcaca
    12661 acggcagcaa agcattacac tatgcgccct gcattcctgg ctactatttc gcaagagcaa
    12721 gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg agtacgattc
    12781 atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg acgtggcgga
    12841 agaagcggat gtgctgctgc tgaacacctg ctcaatccgc gagaaggctc aggaaaaagt
    12901 cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc tgattatcgg
    12961 cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg cccactatgt
    13021 cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca actccgtgcg
    13081 cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt ttgaccgtct
    13141 gccggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag gctgcaataa
    13201 atattgcacc tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc gtccgtccga
    13261 cgatattctg tttgagatcg cccagcttgc ggctcagggc gtgcgtgaag tcaacctgct
    13321 cggtcagaat gtgaatgcct ggcgtggtga gaactacgac ggcaccaccg gatcgtttgc
    13381 cgatctgttg cgtctggttg ctgcgatcga cgggatcgat cgtattcgct ttaccaccag
    13441 ccatccgatc gaattcaccg acgatatcat cgaagtgtat cgcgacacgc cggagctggt
    13501 gagcttcctg catctgccgg tacagagcgg ttccgatcgc attctgaacc tgatgggccg
    13561 tactcatacg gcactggagt acaaagcgat catccgtaaa ctgcgtgcgg cgcgtccgga
    13621 tattcagatc agttctgact tcattgttgg cttccctggc gaaaccaccg aagacttcga
    13681 gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct ttatcttctc
    13741 tgcacgtccg ggtacaccag ccgccgatat ggttgatgat gttccggaag aagagaagaa
    13801 gcaacgtctg tatattctgc aagaacgtat caatcagcaa gcgatggcat ggagccgccg
    13861 gatgctcggc accacccagc gtattctggt agaagggact tcgcgtaaga gcatcatgga
    13921 gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc gagggcacac cggatatgat
    13981 cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc gcggtaaagt
    14041 ggtgcgtact gaagatgaaa tgggtctgcg cgtggcagaa acaccggaat cggtgattgc
    14101 ccgtacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat tctcaggccc
    14161 gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc aatgcttgcg
    14221 cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag cttccggcga
    14281 catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct ggagccagca
    14341 gacaacgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa gcagctcgaa
    14401 cgccgtctcg gcatcgagat caatcgccgc gacaaccact ttaaactgac cggccgtccg
    14461 atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac agccccgatg
    14521 cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa agaagcgcga
    14581 gtactggagc aaagtgcgga gagcgtgccg gagtacggca aagcggtcaa tatcaaaacc
    14641 aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc caatattctc
    14701 gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta cctggcagtg
    14761 gctgcggcag ttgatgccct ggagcgtcag gagattcgcc gtattctgct gactcgtccg
    14821 gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca gaaagtagac
    14881 ccgtatctgc gcccgctgta cgacgcgctg tttgaaatgc tgggctttga gaaagttgag
    14941 aaactgattg agcgcaacgt tattgaagtc gcgccgctgg cctatatgcg tggtcgtacg
    15001 ctgaacgacg cgtttatcat tctcgatgag agccagaaca ccaccatcga acagatgaag
    15061 atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga cgtcacacag
    15121 atcgacctgc cgcgtaatac taaatcaggc ttacgtcacg ccatcgaagt gctggccgat
    15181 gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca cccggtggtg
    15241 gcgcgtatcg ttaacgccta tgaagcctgg gaagaagccg aacaaaaacg aaaagcggcg
    15301 ctggcggcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg atcctcgatt
    15361 tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt caaacatggc
    15421 tgaatgcggt gatcccgcag tttcaggaag aatcggaagt gactattcgc gtggtcgata
    15481 ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg accaacgtgc
    15541 tctccttccc gtttgaagtg ccgcctggca tggaaatgtc gctactgggc gatctggtta
    15601 tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg gaggcgcact
    15661 gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac atcgaagatg
    15721 acgaagcaga agaaatggaa gccctcgaaa cagagattat gcttgctctg ggctatgagg
    15781 atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga cgtccccgcc
    15841 gttggtgatt aacgacggcg ttttgattaa ctaacatgag aacccatacg acgccatgag
    15901 cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt tctccctgtt
    15961 actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg cgctgatccg
    16021 tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg aaggggtgat
    16081 ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga tgattaccct
    16141 gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg cccactcacg
    16201 tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg cgaaagactt
    16261 gctaccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt tacgtcaggc
    16321 ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc gctctcagcg
    16381 ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt tccggtctgg tgaccattga
    16441 agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag aagatgatat
    16501 cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc gcactggctt ccattgaaga
    16561 cttcaacgaa gcgttcggca cccactttag cgatgaagaa gtcgacacta tcggtggtct
    16621 ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa actatcgaca tcgacggtta
    16681 ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt caggttcatg tcaaaatccc
    16741 ggatgactca ccccagccaa agctggatga ataaaaccga aactggatag ataactacat
    16801 ggcttttgcc tcattaattg aacgccagcg cattcgcctg ctgctggcgt tattattcgg
    16861 tgcctgcgga acgctggcct tctctcctta cgatgtctgg cctgcggcga ttgtttcgct
    16921 gatggggctt caggcgctga cctttaaccg ccgcccgctc cagtctgccg ctattggctt
    16981 ctgctgggga tttggcctct ttggcaccgg tattaactgg gtctatgtca gcatcgcgac
    17041 ctttggcggg atgcctggcc cggttaacat cttcctggtg gtgctgctgg cggcgtattt
    17101 gtcgctgtat accggactgt ttgctggcgt gctgtcgcgc ctgtggccga aaaccacctg
    17161 gctgcgcgtg gcgattgccg cccctgctct ctggcaagtg accgagtttc tgcgcggttg
    17221 ggtgctgact ggcttcccgt ggttacagtt cggctatagc cagattgatg gcccgttaaa
    17281 agggctggca ccaataatgg gcgtggaagc cattaacttc ctgctgatga tggttagcgg
    17341 cctgctggca ctggcgttag tcaaacgcaa ctggcgtccg ctggtggtgg ccgtcgtgct
    17401 gtttgccctt cccttcccgc tgcgttacat ccaatggttt accccgcaac cggagaaaac
    17461 cattcaggtt tcgatggttc agggcgatat tccgcaatca ctgaaatggg acggagacca
    17521 gcttcttaat acgctgaaga tttactacaa cgcaacggca ccgctgctgg gcaaatcatc
    17581 gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc aaccgttcct
    17641 caaagcactg gacggtgagt tgcgtgataa aggtagctcg ctggtaaccg ggattgtcga
    17701 cgcgcgtctc aataagcaga accgctacga tacctacaac accatcatca cgctgggtaa
    17761 aggtgcaccg tacagctacg aatcagccga tcgctataac aaaaaccatc tggtgccgtt
    17821 tggcgagttt gtcccgctgg agtcgattct gcgtccgtta gcaccgttct ttgatctgcc
    17881 gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg ctgtcggtaa atggtattga
    17941 gcttactgcg gccatttgct acgagatcat tcttggcgag caagtgcgcg ataacttccg
    18001 cccggatacc gactatctgc tgactatctc caacgatgcg tggtttggta agtctattgg
    18061 tccatggcag cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc gcccactgtt
    18121 gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggtgaga ttcaggcgat
    18181 gatcccgcag ttcacccgcg aggtgttgac cactaatgtg acaccgacca ccggactcac
    18241 accgtatgca cgtaccggca actggccgct gtgggtgctg acggcgctgt ttggttttgc
    18301 cgctgtgctg atgagtctgc gagcacgtaa acgttgatgt gcctgatgta tcctgcatga
    18361 gcaaaacgta ctttctcatt gagctttggg gcatggttat catgcccctt tttcttacgg
    18421 ttcagggata caagccacat tgccatattg gggatgacga ataattgcct gcggatcaag
    18481 cgagcgcgtc ctgtccattt ggatcaaccc gttcttcgcc atgacaaata tttttctacc
    18541 atccgctgaa aaacgaaggt gattaacgta gctggcagga taaacgccaa taatctgccc
    18601 ggcattaata ttgagcagga tgacataacc ttgcaggcca tatgacacca acgctaaata
    18661 ttgaccgtcc gggctaatac cctcaacgga ataaacgctc ctgctacacc attgcgtcat
    18721 aatgctggct tgttgatccc ctacgcttat acgtgaaatc ccctgactat cggcaaaaat
    18781 aacctcgccg ttttcaggtc gaaaacgcat cgccgatatc gttccacgcg catcacctgc
    18841 gggaaagtct tctttctgat gcgtgatggt attgatccgc acgatgttac ctctgcctgc
    18901 tcccggaacc agtaatgttc tttcatccgg ggaaagtgcc ataacccagg cgcctggttt
    18961 accgctcgtc cccgtcccgt cgtatttctc cagcgggata tccgccacat gctgataagt
    19021 caccagatca atcgctgaca cactattact ggtgcgattc atcacccagg cttttgtgcc
    19081 atcacgcgag aggataaaac tgtcaggatg tgcaccggtg gtaattgact gaataatctc
    19141 ccctgtcgcc acgttaatga ccgataacgc attcgcttca aatgacgtcg cataaatcaa
    19201 cttttcatcc gggcttaatg ccagcgatct gacgccctga tctttacagc caccacaatg
    19261 tttgactgac ttttctttcg ctatgggcgc tgtaaaggta tgcaggattt tcttttgtcc
    19321 gaggtcgaaa acaacaatgc tgttggtgtt ttcaattgcg gcatacaacg tattaccatc
    19381 ccgactgatc gccatatcat ggatatcggc aatctcagcg agtaaatcac cactggcctg
    19441 ttcacgactt accggagcgg ataatgtttc cctgagggcg ttgcgttcct ccgctaactg
    19501 ttgttttttt tgtcgtcgct cttgttcaat ctcctgcgga taaaacgctt cacgcaggct
    19561 ttgcctgacc tgtaaaactg actcatccat ctgctgctgc gcaagccaga tagccccggt
    19621 cagaagaaga ctgcccgcaa caataatccc gccttccgta acgagtctgt tttgcccaga
    19681 taaacgcgcg ctcagccaac ccaacgcgcc acccgcaatc aggccgccaa tgtggcaagc
    19741 gttatctatt cctgtttgcc gggcaccgta caacagtgtc agcgccacca tacccaacag
    19801 aggaaatact cgccgctggt ttttatggta tgtgcccgta cctgaagcaa actgtgttgc
    19861 tatcgctgca ccagctattc ccagaatcgc cccggatgcc ccaacgctga tgactatctg
    19921 ctcactttcg cgtaacgcat aacaggcgct gatgagccca ccaaagacgc cggagaataa
    19981 ccaggtaatc aggagtcgcc atttccccag aatctgttcc gccacgcctc ccacgaccag
    20041 gaatgcaaac atattcatca gcagatgaga aacactactg tgcagaaaga tactgctcaa
    20101 taagcgccag ggttcacccg acaaagtaag aggcgcaacg tttgcaccga gatggataag
    20161 tgcggattga tcgacctctt cataactcat ccattgtgcg agataacaaa atatcccaat
    20221 acagagtgca aaaaggataa gggttattgc gggtaattga acatttaacg gcttaaccga
    20281 agatgcagac ataaggtgtt tcttccttga aaagacaggt aaaaagcatt tttcccgtgt
    20341 gcagttatca tcccggcaaa cgtcccatgc cgaaaagata attctgtccg aaatatgact
    20401 ttttcagcct aaaattacaa tcgtaattaa ttcgccccta agtatttttt ggcatgtaag
    20461 ttgctttatt tactgtgtaa gcgatgaaat cagccgcgac gcaaccaggt tgcaccacca
    20521 caaatcagcg gtagcaccaa aaaggtgcgg tttaagaatt ttgcttcgga atggtgcggc
    20581 gcatctcgca aaaacaaaca ataaaacagc aatatcttta catttagcca aactaaatgt
    20641 tacctaacag gcacaacact gcacaataaa gttgcagacg ataacaacac aaacactcac
    20701 aacgggtatc catgcgttct taacgcagaa gataaaggag ttggatatgc aattacgtaa
    20761 acctgccaca gcaatcctcg ccctggcgct ttccgcagga ctggcacagg cagatgacgc
    20821 cgccccggta gcgggcagta cgctggacaa aatcgccaaa aacggcgtga ttgtcgtcgg
    20881 tcaccgtgaa tcttcagtac ctttctctta ttacgacaat cagcaaaaag tggtgggtta
    20941 ctcgcaggat tactccaacg ccattgttga agcagtgaaa aagaaactca acaaaccgga
    21001 cttgcaggta aaactgattc cgattacctc acaaaaccgt attccactgc tgcaaaacgg
    21061 cactttcgat tttgaatgtg gttctaccac caacaacgtc gaacgccaaa aacaggcggc
    21121 tttctctgat actattttcg tggtcggtac gcgcctgctg accaaaaagg gcggcgatat
    21181 caaagatttt gccgacctga aaggcaaagc cgtagtcgtc acttccggca ctacttctga
    21241 agttttgctc aacaaactga atgaagagca aaaaatgaat atgcgcatca ttagcgccaa
    21301 agatcacggt gactctttcc gtaccctgga aagcggtcgt gccgttgcct ttatgatgga
    21361 tgatgccctg ctggccggtg aacgtgcgaa agcgaagaaa ccagacaact gggatatcgt
    21421 cggcaagccg cagtctcagg aagcctacgg ttgcatgttg cgtaaagatg atccgcagtt
    21481 caaaaagctg atggatgaca ctatcgctca ggtgcagact tccggtgaag cggaaaaatg
    21541 gtttgataaa tggttcaaaa atccaattcc gccgaaaaac ctgaacatga atttcgaact
    21601 gtcagacgaa atgaaagcac tgttcaaaga accgaatgac aaagcactga actaattaca
    21661 agaaccaggg gcggaaattc cagccctctc gattgttacg tagcacggac agactatacg
    21721 cctgatggtc gttccccatc gggcctgaaa accgcaatac gctgggtaac aatcttcgag
    21781 ggtagcagtt aacgctgcta ccctcttttt tctggagtag atttatgtct atagactgga
    21841 actggggtat ttttttacaa caagccccgt tcggcaacac cacctatctt ggttggatct
    21901 ggagtggttt tcaggtgacg atcgctttgt cgatctgcgc ctggattatc gctttcctcg
    21961 tcggttcatt tttcggcatt ttacgtaccg ttccaaaccg ttttctctcc ggtctgggta
    22021 cgttgtatgt cgaactgttc cgcaacgtgc cgttgattgt gcaattcttt acctggtatc
    22081 tggtgatccc ggagttgctg ccagagaaaa tcggtatgtg gtttaaggcc gagctggatc
    22141 ccaatattca gtttttcctt tcatccatgc tctgcctggg gctgtttacc gccgcccgtg
    22201 tttgcgaaca ggtacgcgcc gccattcagt cgttgccgcg tgggcaaaaa aatgccgcgc
    22261 tggcgatggg cctgacgcta ccgcaggctt atcgatacgt actgctgcct aatgcttatc
    22321 gcgttatcgt cccgccgatg acttccgaga tgatgaacct ggtgaaaaac tccgccatcg
    22381 cctcgactat tggtctggtg gatatggcgg cgcaggcggg taaattgctt gattactcag
    22441 cccacgcctg ggagtcgttt accgctatta cgctggcgta tgtgttgatt aacgcggtta
    22501 tcatgctggt aatgacgctg gttgaacgta aagttcgcct gcctggcaac atggggggca
    22561 aataatgtac gagtttgact ggagttccat tgtcccttcc ctgccatatc tgctcgacgg
    22621 gctggtgatc acgctgaaaa tcaccgtcac ggcagtcgtg attggtattt tgtggggcac
    22681 catgctggcg gtgatgcgct tgtccagctt tgcgcccgta gcgtggtttg ccaaagccta
    22741 cgttaacgta ttccgctcga ttcctttagt tatggttttg ctgtggtttt acctgatcgt
    22801 gccaggtttt ctgcaaaacg tgctgggatt atcaccaaaa aatgatatcc gtttaatttc
    22861 ggcgatggtg gcgttttcaa tgtttgaagc ggcctactat tcagagatta tccgtgctgg
    22921 tattcaaagt atttctcgtg gtcagtcgag cgccgcgctg gcgttgggaa tgactcactg
    22981 gcagtcgatg aaactgatta ttctgccgca ggcgttccgc gcgatggtgc cgcttctgct
    23041 cactcagggc atcgtactgt tccaggatac ctcactggtg tatgtgttat ctctggccga
    23101 tttcttccgt accgcctcaa ccattggtga gcgtgatggt acgcaggttg agatgatcct
    23161 atttgccgga tttgtttatt tcgttattag tcttagcgcg tcgttgttgg tcagctactt
    23221 gaaaagaagg acagcataat gattaccctg aaaaatgttt caaaatggta tggtcacttt
    23281 caggtgctga ccgactgctc aaccgaagtg aaaaaaggcg aagtggtggt ggtttgcggc
    23341 ccgtctggtt ccggcaaatc aacgctgatt aaaaccgtca acggcctcga accagtgcag
    23401 caaggtgaaa tcaccgtcga tggtatcgtg gttaatgaca agaaaaccga tctggcaaag
    23461 ctgcgttccc gcgtcgggat ggtgttccag cattttgagt tgttccccca tttgtcgatt
    23521 attgaaaacc tgactctggc gcaggtgaaa gtgcttaaac gcgataaagc gcccgctcgt
    23581 gaaaaagcat tgaaattgct ggagcgcgtg ggactttccg cacatgccaa taaatttccg
    23641 gcgcagcttt ccggcggtca gcagcagcgt gtggcaatcg ctcgcgcgtt gtgtatggat
    23701 cctattgcga tgctgttcga cgaaccgaca tcggcgctgg atccggagat gatcaacgaa
    23761 gtgctggacg tgatggtgga actggcgaac gaaggaatga ccatgatggt ggtgacccac
    23821 gaaatgggct ttgcccgcaa agtggcgaat cgggtgatct ttatggacga gggtaaaatt
    23881 gtcgaagact cgccgaaaga cgctttcttc gatgatccga aatcggaccg cgcgaaagac
    23941 ttcctcgcga aaatcctgca ttaatcactc tggcgcgcat acccaagtgc gcgccatctc
    24001 acgctttatc gtttcgcccc tcgtatctgt tacatcctgg cgttaccctt gtcgcaaaga
    24061 agcacacaac aaggagcaac aatggcactg ccaattctgt tagattgcga cccaggtcat
    24121 gacgacgcta tcgcaatagt tctcgccctc gcctcaccag agcttgatgt caaagcaatt
    24181 acgtcttccg ccggaaacca gacaccagaa aaaaccttac gcaatgttct gcgtatgctg
    24241 accttgctta atcgcaccga tattccggta gcaggcggcg cggtaaaacc gttaatgcgt
    24301 gagttgatta tcgcggacaa tgtgcacggc gaaagcggtc tggatggccc ggcattaccg
    24361 gaaccgacgt tcgcaccgca aaactgtacg gcggtagagc tgatggcgaa aacgctgcgc
    24421 gaaagtgcgg aacctgtcac gattgtgtct accggaccgc aaactaacgt tgccttgctg
    24481 ctcaatagcc atccggaact gcatagcaaa attgcccgta tcgtgattat gggcggcgca
    24541 atggggcttg gtaactggac gcctgcggct gaatttaata tttacgttga cccggaagcg
    24601 gcagaaattg tcttccagtc agggatccca gtggtgatgg ccggtctgga tgttactcac
    24661 aaagcacaaa tccacgttga agacaccgag cgtttccgcg cgattggcaa ccctgtttca
    24721 accattgttg ccgaactgct ggatttcttc ctcgaatatc ataaagacga aaaatggggc
    24781 tttgtcggtg caccgctgca tgacccatgc accatcgcct ggctgttgaa acccgagttg
    24841 tttaccactg ttgagcgctg ggttggcgtg gaaacacagg ggaaatatag ccagggtatg
    24901 acggttgttg attattatta tctgactggc aataaaccga atgccactgt aatggtcgat
    24961 gttgatcgtc agggctttgt tgatttactg gctgatcgtc tgaaatttta cgcttaacac
    25021 acgtcattga ttaagatcac cacgcgttac ctgggtggtc ttaatctgtt tatctctttt
    25081 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    25141 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    25201 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    25261 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    25321 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    25381 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    25441 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    25501 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    25561 ctgcgatggc gtatggcctg catacccaac aaaatacccg ttcgctggtg tttgatctcg
    25621 gtggcggcac gtttgacgtt acggtgcttg agtacgccac gccggtgatt gaagttcacg
    25681 cctccgctgg cgacaacttt cttggtggcg aagattttac ccatatgctg gtcgatgagg
    25741 ttttaaaacg cgcggatgtc gccaggacca cgcttaacga gagtgaactg ggagccttgt
    25801 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    25861 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    25921 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    25981 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    26041 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    26101 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    26161 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    26221 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    26281 cttactcgac catgcacccg gaacagaatt caattacggt taacgtctat cagggagaaa
    26341 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    26401 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    26461 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    26521 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    26581 gcgatatgct catcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    26641 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    26701 caaacgatat ggcccgcgtc gatgaggtca ggcggcgggc gagcgtttat ttagccattg
    26761 agacctcata atcacggctg ccggatgcgg cgtaaatgct gcatccggca tcggcgcaaa
    26821 aaacacatcg tcaactaaac aacatcgcga ttttgccgag agcataacct ataaccagca
    26881 ccgttaaact gattttgata atgctggtaa tgctctgtgg tttttcaccc tcatcatttt
    26941 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttta
    27001 ctttcgtcaa cagcatccgg gtgataatgg cctctaatgg atgagcatct ccgccttcca
    27061 gccttgtatt aagacgggcc agcatctctt gtttcggaaa cgcttcaagt actacctgtt
    27121 ccgctacccg atacaaatcc agtaacggtg aatcttcttc tgtctcgcta ccgactctcg
    27181 atttccacca cgcccatgct ttgcgtttga agaataacga ctcaataaac gattgaccta
    27241 acggcgataa ttttccgcta tgtaaaattt ccgcccataa cagccgcgtc cgggcgctct
    27301 caccaggaac atatttcagc ggttgacctt gcgcatctac catgctccgc gcttcgataa
    27361 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    27421 tatcagcttg tgtagatggg taactttccc ggtacgcgca gagatcggca agcaggcttt
    27481 ccccctcgcc gcagtacaac ctttgagcac acaagtaata tcgggcatct tcgctttctg
    27541 gttcttccat ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    27601 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggac
    27661 cttccatcgc caaaaacgca gtgacgtagt caggatggta acggcagcaa cgttccagcg
    27721 cgaagtaata ctcaacagtc tggttctgca acgcaacggg atattccaga aggctaagga
    27781 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    27841 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccagcaaa gaagcacaat
    27901 ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    27961 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    28021 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg cagtatttca tgttcagcgg
    28081 cggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt cgcagcgcct
    28141 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacataacg
    28201 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    28261 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    28321 ctggacagtt aatggctgcc agggaagcgt ggcatagaaa cgatcgcttc ccgctgccag
    28381 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    28441 cgaagtgttg tcttcgcact gagaagattg ccagtagaga cgtcctaacg aataggcgga
    28501 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt acatagacgc acgattgcag
    28561 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg acagaaaccc
    28621 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    28681 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttccactc
    28741 ttcaggggaa gagcgatgcc atgtcagata taagccagct aaatcattgg catgaccatc
    28801 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    28861 tgagagatcg aattctttta tcccggtata catttttggc gagtcatgcg cgggtttgca
    28921 accctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    28981 attcatttgt taataatccc ttaagtg
//
LOCUS       DAZKGS010000050        28691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKGS010000050 DAZKGS010000000
VERSION     DAZKGS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..1195
                     /gene="ompC"
                     /locus_tag="ACHOQY_002095"
     CDS             104..1195
                     /gene="ompC"
                     /locus_tag="ACHOQY_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9678190.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1307..2362
                     /gene="apbE"
                     /locus_tag="ACHOQY_002096"
     CDS             1307..2362
                     /gene="apbE"
                     /locus_tag="ACHOQY_002096"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9678191.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2436..3500
                     /gene="ada"
                     /locus_tag="ACHOQY_002097"
     CDS             2436..3500
                     /gene="ada"
                     /locus_tag="ACHOQY_002097"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9678192.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3500..4150
                     /gene="alkB"
                     /locus_tag="ACHOQY_002098"
     CDS             3500..4150
                     /gene="alkB"
                     /locus_tag="ACHOQY_002098"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9678193.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4226..5869
                     /gene="yojI"
                     /locus_tag="ACHOQY_002099"
     CDS             4226..5869
                     /gene="yojI"
                     /locus_tag="ACHOQY_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9678194.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6087..7733
                     /gene="mqo"
                     /locus_tag="ACHOQY_002100"
     CDS             6087..7733
                     /gene="mqo"
                     /locus_tag="ACHOQY_002100"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9678195.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(7882..8391)
                     /gene="eco"
                     /locus_tag="ACHOQY_002101"
     CDS             complement(7882..8391)
                     /gene="eco"
                     /locus_tag="ACHOQY_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9678196.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(8506..8670)
                     /gene="yojO"
                     /locus_tag="ACHOQY_002102"
     CDS             complement(8506..8670)
                     /gene="yojO"
                     /locus_tag="ACHOQY_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP9678197.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            8779..9273
                     /gene="napF"
                     /locus_tag="ACHOQY_002103"
     CDS             8779..9273
                     /gene="napF"
                     /locus_tag="ACHOQY_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9678198.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9263..9526
                     /gene="napD"
                     /locus_tag="ACHOQY_002104"
     CDS             9263..9526
                     /gene="napD"
                     /locus_tag="ACHOQY_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9678199.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9523..12009
                     /gene="napA"
                     /locus_tag="ACHOQY_002105"
     CDS             9523..12009
                     /gene="napA"
                     /locus_tag="ACHOQY_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFP9678200.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12016..12711
                     /gene="napG"
                     /locus_tag="ACHOQY_002106"
     CDS             12016..12711
                     /gene="napG"
                     /locus_tag="ACHOQY_002106"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9678201.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            12698..13561
                     /gene="napH"
                     /locus_tag="ACHOQY_002107"
     CDS             12698..13561
                     /gene="napH"
                     /locus_tag="ACHOQY_002107"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9678202.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13558..14007
                     /gene="napB"
                     /locus_tag="ACHOQY_002108"
     CDS             13558..14007
                     /gene="napB"
                     /locus_tag="ACHOQY_002108"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9678203.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14017..14619
                     /gene="napC"
                     /locus_tag="ACHOQY_002109"
     CDS             14017..14619
                     /gene="napC"
                     /locus_tag="ACHOQY_002109"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9678204.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14632..15255
                     /gene="ccmA"
                     /locus_tag="ACHOQY_002110"
     CDS             14632..15255
                     /gene="ccmA"
                     /locus_tag="ACHOQY_002110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9678205.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15252..15914
                     /gene="ccmB"
                     /locus_tag="ACHOQY_002111"
     CDS             15252..15914
                     /gene="ccmB"
                     /locus_tag="ACHOQY_002111"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9678206.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            15956..16693
                     /gene="ccmC"
                     /locus_tag="ACHOQY_002112"
     CDS             15956..16693
                     /gene="ccmC"
                     /locus_tag="ACHOQY_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9678207.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16690..16899
                     /gene="ccmD"
                     /locus_tag="ACHOQY_002113"
     CDS             16690..16899
                     /gene="ccmD"
                     /locus_tag="ACHOQY_002113"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9678208.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            16896..17375
                     /gene="ccmE"
                     /locus_tag="ACHOQY_002114"
     CDS             16896..17375
                     /gene="ccmE"
                     /locus_tag="ACHOQY_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9678209.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17372..19315
                     /gene="ccmF"
                     /locus_tag="ACHOQY_002115"
     CDS             17372..19315
                     /gene="ccmF"
                     /locus_tag="ACHOQY_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9678210.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19312..19869
                     /gene="dsbE"
                     /locus_tag="ACHOQY_002116"
     CDS             19312..19869
                     /gene="dsbE"
                     /locus_tag="ACHOQY_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9678211.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            19866..20918
                     /gene="ccmH"
                     /locus_tag="ACHOQY_002117"
     CDS             19866..20918
                     /gene="ccmH"
                     /locus_tag="ACHOQY_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9678212.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(20953..21600)
                     /gene="narP"
                     /locus_tag="ACHOQY_002118"
     CDS             complement(20953..21600)
                     /gene="narP"
                     /locus_tag="ACHOQY_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9678213.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            21920..24511
                     /gene="yejO"
                     /locus_tag="ACHOQY_002119"
                     /pseudo
     CDS             21920..24511
                     /gene="yejO"
                     /locus_tag="ACHOQY_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(24979..25902)
                     /locus_tag="ACHOQY_002120"
     CDS             complement(24979..25902)
                     /locus_tag="ACHOQY_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9678214.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            complement(25892..26053)
                     /locus_tag="ACHOQY_002121"
     CDS             complement(25892..26053)
                     /locus_tag="ACHOQY_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678215.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            complement(26065..26553)
                     /locus_tag="ACHOQY_002122"
     CDS             complement(26065..26553)
                     /locus_tag="ACHOQY_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9678216.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            complement(26681..26842)
                     /locus_tag="ACHOQY_002123"
     CDS             complement(26681..26842)
                     /locus_tag="ACHOQY_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFP9678217.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            complement(26846..27040)
                     /locus_tag="ACHOQY_002124"
     CDS             complement(26846..27040)
                     /locus_tag="ACHOQY_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678218.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            complement(27171..27416)
                     /locus_tag="ACHOQY_002125"
     CDS             complement(27171..27416)
                     /locus_tag="ACHOQY_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678219.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            complement(27618..>28691)
                     /locus_tag="ACHOQY_002126"
     CDS             complement(27618..>28691)
                     /locus_tag="ACHOQY_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP9678220.1"
                     /translation="KRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDY
                     VVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGI
                     GVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIIL
                     DTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRA
                     SLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVV
                     DKPRPPVELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHF
                     RRWLYKLENDGVIVIDGDDVRPL"
BASE COUNT         6504 a         7160 c         7862 g         7165 t
ORIGIN      
        1 tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca gtatgcagtg
       61 gcataaaaaa gcaaataaag gcatataaca gagggttaat aacatgaaag ttaaagtact
      121 gtccctcctg gtcccagctc tgctggtagc aggcgcagca aacgctgctg aagtttacaa
      181 caaagacggc aacaaattag atctgtacgg taaagtagac ggcctgcact atttctctga
      241 caacaaagat gtagatggcg accagaccta catgcgtctt ggcttcaaag gtgaaactca
      301 ggttactgac cagctgaccg gttacggcca gtgggaatat cagatccagg gcaacagcgc
      361 tgaaaacgaa aacaactcct ggacccgtgt ggcattcgca ggtctgaaat tccaggatgt
      421 aggttctttc gactacggtc gtaactacgg cgttgtttac gacgtaactt cctggaccga
      481 cgtactgcca gaattcggtg gcgacactta cggttctgac aacttcatgc agcagcgtgg
      541 taacggtttc gcgacctacc gtaacactga cttcttcggt ctggttgacg gcctgaactt
      601 tgctgttcag taccagggca aaaacggtag cgtaagcggc gaaggcatga ctaacaacgg
      661 tcgtgaagca ctgcgtcaga acggcgacgg cgttggcgga tctatcactt atgattacga
      721 aggcttcggt atcggtgctg cagtttccag ctccaaacgt actgatgatc agaacagccc
      781 gctgtacatc ggtaacggcg accgtgctga aacctacact ggtggtctga aatacgacgc
      841 taacaacatc tacctggctg ctcagtacac ccagacctac aacgcaactc gcgtaggttc
      901 cctgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc agttcgactt
      961 tggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg gtcgtggcta
     1021 cgacgacgaa gatatcctga aatatgttga tgttggtgcg acctactact tcaacaaaaa
     1081 catgtccacc tatgttgact acaaaatcaa cctgctggac gacaaccagt tcactcgtga
     1141 cgctggcatc aacactgata acatcgtagc tctgggtctg gtttaccagt tctaatctcg
     1201 attgatatcg aacaaagggc ctgcgggccc ttttttcatt gttttcagcg tacaaacaca
     1261 gttttttggt gtactcttgc gaccgttcgc atgaggataa tcacgtatgg aaataagctt
     1321 tacccgcgtg gcactgctgg ctgccgcgct cttctttgtt ggttgcgatc aaaaaccaca
     1381 aaccgccaaa acccacgcta ctgaagttac cgttcttgaa ggcaaaacta tgggtacctt
     1441 ctggcgtgcc agcatcccgg gcattgacgc caaacgcagt gccgaactta aagaaaagat
     1501 tcagacccag ctggacgccg acgatcagct gctttcgacc tataaaaaag attccgcgct
     1561 gatgcgcttt aacgattcgc aaagtttatc gccgtggccg gtaagtgaag cgatggccga
     1621 tatcgtcacc acctcgctgc gcattggcgc aaggaccgat ggtgcgatgg atataaccgt
     1681 cgggccgctg gtgaatctgt ggggctttgg cccggaacaa cagccggttc aaattccgag
     1741 ccaggaacag atcgatgcga tgaaagccaa aaccggctta cagcacctga cggtcattaa
     1801 tcagtcgcat cagcaatatc tgcaaaaaga cctaccggat ttatatgtcg atctctccac
     1861 cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg atggagcagg aagggatttc
     1921 ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt ggtatgaacg gtgaaggcct
     1981 gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa aacgcggttc aggctgtggt
     2041 ggatatcaac ggtcatggta ttagcacctc tggcagttac cgtaactatt acgaactgga
     2101 cggcaaacgt ctttctcatg ttatcgatcc gcaaaccggg cgtcccatcg aacacaatct
     2161 ggtatccgtg acggtgattg ctccgacggc gctggaagcc gatgcctggg atactggctt
     2221 gatggtactc gggccggaga aagccaaaga ggttgttcgc cgggaagggc tggcggttta
     2281 tatgatcacc aaagaaggcg atagctttaa aacctggatg tcaccacagt ttaaaagctt
     2341 ccttgtcagc gaaaaaaatt aaagcgcaag attgctggtt tttgcgtgat ggtgaccggg
     2401 cagactaaag gctatcctta accagggagc tgattatgaa aaacgccaca tgcttaactg
     2461 acgatcaacg ctggcaatct gtcttagccc gcgacccgaa tgccgacagc gaattcgttt
     2521 tcgccgtgcg caccacaggc atcttttgcc gtccgtcttg ccgcgccaga catgccttgc
     2581 gggaaaatgt ctccttctac gcaaatgcct gcgaggcact cgccgccgga tttcgcccct
     2641 gcaaacgctg ccaacctgat aaagccaatg cccagcaaca tcggttggat aaaatcaccc
     2701 acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct ggaagcctta gccgaccagg
     2761 tggcgatgag tccatttcat ctacatcggt tgtttaaagc gactaccgga atgacgccta
     2821 aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga atcgctggcg aaaggggaga
     2881 gcgtgacgac gtctattctt aacgccggat tccccgacag cagcagttac tatcgcaaag
     2941 ctgacgaaac gctgggcatg acggctaaac aattccgtca cggtggcgaa aatctggcgg
     3001 tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct ggtggcagaa agcgagcggg
     3061 ggatttgcgc gatattgctg ggcgatgatg acgcgacact aatcagcgag ttgcagcaga
     3121 tgtttcccgc tgccgacaac gcgcctgccg atctgatgtt tcagcaacat gtgcgtgaag
     3181 tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt accgctggac attcgcggca
     3241 ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat accttgcggt gaaaccgtca
     3301 gttatcagca actggctaac gccatcggca aaccgaaagc ggtacgggcc gttgccagcg
     3361 cctgtgccgc caacaagctg gctatcataa taccctgtca tcgggtggtc cgtggtgatg
     3421 gcacactttc cggttaccgc tggggcgtgt cgcgtaaagc gcaactgctg cgccgcgaag
     3481 ctgaaaatga ggagaggtaa tgttggatct gtttgccgat gctgaaccgt ggcaagagcc
     3541 actggcggct ggagcggtaa ttttgcaccg ttttgctttt aacgctgcgg agcaactgat
     3601 ccgcgatatt aatgacgttg ccagccagtc gccgtttcgc cagatggtca cccccggggg
     3661 atataccatg tcggtggcga tgaccaactg tgggcatctg ggctggacga cccatcggca
     3721 aggttatctt tattcgccca ttgatccgca aacaaataaa ccgtggcccg ccatgccaca
     3781 aagctttcat gatttatgtc aacgtgcggc tacggcggcg ggctatccgg atttccagcc
     3841 agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa
     3901 agacgaaccg gatctgcgcg cgccgattgt ttctgtttct cttggcttac ccgcgatttt
     3961 tcaatttggt ggcctgaaac gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga
     4021 tgtggtggta tggggcggtg aatcgcggct gttttatcac ggcattcaac cgctgaaagc
     4081 ggggtttcat ccactcaccg gcgactgccg ctacaacctg acattccgtc aggcaggtaa
     4141 aaaagaataa aaataagaat tattattgct gtgcgcgcga agatgtttaa actgcgggct
     4201 gtaattcatt gtccgggttt tctgcatgga acttcttgta cttgtctggc ggcagtatcg
     4261 ctggccattt atcagtgtga tggcgctaag cctcgccagt gcggcattag gcattggctt
     4321 aattgctttt atcaatcagc gccttatcga aacggcggat accagtctgc tggtattgcc
     4381 ggagtttctg ggattattgc tgctgttgat ggcagtcact ctcggatcgc aactggcgct
     4441 caccactttg gggcatcact tcgtttaccg actgcgtagt gaatttatca agcggattct
     4501 ggataccaac gtcgagcgca ttgaacaact cggtagcgcc tcgttgctgg cgggattaac
     4561 cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctt ccggaactgg tgcaggggat
     4621 cattctcact atcggttccg cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt
     4681 aacggctatc tggatggcga tcaccatctg gggtggtttt gtactggtgg cgcgggtgta
     4741 caaacatatg gcgaccctgc gtgaaaccga agacaggctg tatacggatt ttcaaactgt
     4801 actcgaaggg cgcaaagagc tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa
     4861 cctctacatt cctgatgcgc aagagtatcg ccaccatatt atccgcgcag acaccttcca
     4921 tcttagcgcc gtgaactggt caaacatcat gatgttgggc gcaatcggcc tggtgttctg
     4981 gatggcgaac agtctcggtt gggctgatac caacgttgcc gcgacctatt cgttgacgct
     5041 tttattcctg cgtacgccgc tgctttcggc ggttggcgca ttgccgacgc tgctgacggc
     5101 gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg cctttcaaag cagagtttcc
     5161 gcgcccgcag gcgtttccca actggcaaac gctggagctg cgtaacgtga cgtttgctta
     5221 tcaggataac gcgttttccg ttggtccgat taatctcacc atcaaacgtg gcgagctgct
     5281 gtttctgatt ggcggcaacg gtagcggaaa atcgacgctg gcgatgttgt tgacgggctt
     5341 gtatcagcca caaagcggcg aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc
     5401 ggaagattat cgcaaactgt tttcggcagt gtttaccgat gtctggctgt ttgatcaact
     5461 gctggggccg gagggtaaac ccgctaaccc gcaactggtt gagaagtggc tggcgcagct
     5521 gaaaatggct cataagcttg agttaagcaa cgggcgtatt gttaacctga agttatcaaa
     5581 agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca gaagaacgcg atattatcct
     5641 gctggatgaa tgggcggcgg atcaggatcc acacttccgt cgtgagtttt atcaggtgtt
     5701 gctgccgctg atgcaggaga tgggtcaaac tattttcgcc atcagtcatg atgatcatta
     5761 ctttatccac gccgaccgcc tgctggaaat gcgcaacggg caacttagcg agctgacggg
     5821 cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg acggcataat attacgacgc
     5881 tgacctggcg gactgctgcc gtcaggtcaa tatgctaaaa atcccttcat ttctaataac
     5941 cctataatta atcaacgaaa ttataatgtt tctaaaatta gaatataatt tataaacatt
     6001 atttaaatgt tgttacttaa gtgttaccgt tgatgccgcg caaatctcac ctgcaataaa
     6061 gcgactaaaa gtaaggcatt aacaagatga aaaaagtgac tgccatgctc ttctcgatgg
     6121 ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa agcgtccgag gagcaggaaa
     6181 ctgatgtact gttgattggc ggcggcatta tgagcgccac gttggggacc tatttacgcg
     6241 agctggagcc tgaatggtcg atgaccatgg tggagcgcct ggagggtgtc gcgcaggaga
     6301 gttcgaacgg ctggaataac gccggaaccg ggcattctgc actgatggaa ctgaactaca
     6361 ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc agtcgccatt aacgaagcat
     6421 ttcagatttc ccgccagttc tgggcgcacc aggttgagcg cggcgtgctg cgtactccgc
     6481 gttcatttat caataccgtt ccgcatatga gctttgtctg gggcgaggat aacgttaatt
     6541 tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct gtttcgcggt atgcgttact
     6601 ctgaagatca cgcgcagatc aaagagtggg caccgttagt gatggaaggg cgcgatccac
     6661 aacagaaagt agcggcaaca cgcacagaaa ttggcactga tgtgaactat ggcgagatca
     6721 cccgccagtt aattgcttcc ttacagaaga aatctaactt ctcgctgcaa ctcagcagcg
     6781 aagtccgcgc cctaaagcgt aatgacgata acacctggac cgttaccgtt gccgatctga
     6841 aaaacggcac tgcacagaac atccgcgcca aatttgtctt tatcggcgcg ggcggtgcgg
     6901 cgctgaagct attacaggaa tcggggattc cggaagcgaa agactacgcc ggtttcccgg
     6961 tgggcggaca gttccttgtt tcggaaaacc cggacgtggt taatcaccat ctggcgaagg
     7021 tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt tccgcatatc gatacccgcg
     7081 ttctggacgg taaacgcgta gtgctgtttg ggccatttgc caccttctca accaaattcc
     7141 tcaaaaacgg ttcattgtgg gatctgatga gttccaccac cacctctaac gtgatgccga
     7201 tgatgcacgt cgggctggat aatttcgatc tggtgaaata tctggtgagt caggtgatgt
     7261 tgagtgaaga ggatcgtttt gaagcgttga aagagtacta tccgcaggca aaaaaagagg
     7321 actggcgttt gtggcaagcg gggcagcgcg tgcagattat caagcgtgat gccgagaaag
     7381 gtggcgtact gcgtctgggt actgaagtgg tcagtgacca gcaagggacc attgccgcgc
     7441 tgctgggggc atcgccaggg gcgtcaaccg ccgcgccgat tatgttggat ctgctggaaa
     7501 aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc
     7561 cgtcttatgg acgcaagctg aacggtgatg tagcggcaac agaacgcgag ttgcagtaca
     7621 ccagcgaagt gctggggctg aaatacgaca agccgcaggc agcagatagt acgccaaaac
     7681 cgcagttgaa accgcaaccc gttcaaaaag aagtggcgga tattgcgttg taatgatgtg
     7741 ccacatccgg catggtatgc cggatgtggc gtatgctgat aagacgcgcc agcgtcgcat
     7801 caggcaccgg ctcgggcgtt agatgtcgga tgcggcgtga ccgccttatt cggcctacgg
     7861 ggtgccgcac ttcacagcat tttagcgaac taccgcgttg tcaattttct cttccgcctt
     7921 ccagacgcgg tacttcacat ctacattgtc tggcgtatac accacgatcg gcagcttgct
     7981 gttgtaacgc agcattccag catcgcccag atacgcggtg acaaatttct tctctttctt
     8041 gccatccggg caggccatca tcgtggaaac cggcgagcta actttatcaa agacataata
     8101 atcatagccc cagccttcca gcgttttgct ttccagcttc ccgccgagac gatgcaaatt
     8161 gcaatcgact tccagcgtct gaccgattaa cagttctact ttcagggtag attcatcttc
     8221 ttgcggggtt aactgaatca cctgacgctt catccctttt tcagcttgtg gataaggcgc
     8281 gattttttcc agtggctgga cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc
     8341 aaacaatact gcaggtagaa tggtcttcat ttttttgctt tccttcttca atttataggt
     8401 tttcaccgat gatgtcgctg aaggttaaca tattcatcag atttattaat ttgctttatg
     8461 aataacttaa agaacattca gacaatttta cctgctcttt tagtgttaaa ttctgatgag
     8521 ggagcaaacc agcagaaata tctctggtga attaatcgct atataaatat gtttataacc
     8581 atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc cgccactctt ttgatcctgc
     8641 tacaggtttt accccgatcg gggtatgcat ctttgacaca tcctttaata tcttagcggc
     8701 tataaaaatg gcttattaat tatgcggttt atttggtcgc tctcaatttt cagagcgcgt
     8761 taatgatgga aggtcaatgt gaagattgat gcatcccgtc ggggcatact cactggtcgc
     8821 tggcgcaaag ccagtaacgg tatccgtccg ccctggtccg gtgatgaatc tcattttctg
     8881 acccattgca cccgctgcga cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc
     8941 ggcgcgggcg gctatccgag cgttaatttc aaaaataacg agtgcagctt ctgttacgcc
     9001 tgcgcgcagg cttgccccga atcgctattt tctccgcgcc acaccagggc ctgggatttg
     9061 cagttcacca ttggggacgc gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag
     9121 gatagctgtg agccaatggc aattatcttt cgcccgacgc tctccgggat ctaccagccg
     9181 caacttaata gccaactttg caacggatgt ggagcatgtg cagccagctg tccggtatca
     9241 gccataactg cggagtatct ccatgcacac taactggcaa gtttgcagcc tggtcgtgca
     9301 ggccaaaagc gaacgaattt cagacatcag cacccaactg aacgcctttc ccggctgtga
     9361 agttgctgtc agcgacgcgc cgagcggtca gttgattgtg gtggtggaag cagaagacag
     9421 cgaaacgctg atccaaacca ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc
     9481 gctggtttat caccagcagg aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt
     9541 agctttatga aagctaacgc cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg
     9601 ggcgttgccc gcgccgttgt tggtcagcag gaagccatta aatgggataa agcgccgtgc
     9661 cgtttctgcg gtactggttg cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc
     9721 tgtcagggcg acccggacgc accggttaac cgtggcctga actgcattaa gggctatttc
     9781 ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac
     9841 ggtaaatatg acaaagaagg cgaatttacc ccaatcacct gggatcaggc cttcgatgtg
     9901 atggaagaga aattcaaaac cgccctgaaa gaaaaaggac cggaatcgat cggtatgttc
     9961 ggttctggtc agtggactat ctgggaaggt tatgccgcgt ccaagctgtt caaagcgggc
    10021 ttccgttcga acaacatcga cccgaacgca cgtcactgta tggcgtcggc agtagttggc
    10081 tttatgcgta cctttggtat ggatgagccg atgggctgct atgacgacat cgagcaggct
    10141 gacgcgtttg tgctgtgggg cgctaacatg gcggagatgc acccgatcct ctggtcacgc
    10201 atcactaacc gtcgtctctc taaccagaac gtcaccgtgg cggtgctttc tacctaccag
    10261 catcgtagct tcgagctggc ggataacggc atcatcttta cgccacagtc tgacctggtg
    10321 atcctgaact acatcgccaa ctatatcatt caaaacaatg cgataaatca ggacttcttc
    10381 agcaagcacg ttaacctgcg caaaggggcg acggacatcg gctacggttt acgtccgacc
    10441 catccgctgg aaaaagcagc gaagaatccg ggttctgacg cctccgaacc gatgagcttt
    10501 gaagattaca aagccttcgt tgccgagtat acgctggaaa aaactgccga aatgaccggc
    10561 gtgccgaaag accagttaga acaactggcg cagctgtatg ccgatccgaa caagaaagtc
    10621 atctcctact ggacgatggg cttcaaccag catactcgtg gcgtgtgggc caacaacctg
    10681 gtctacaacc tgcacctgct gaccggcaaa atttcccagc cgggttgcgg tccgttctcc
    10741 ctgaccgggc agccttccgc gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt
    10801 ctgcctgcgg acatggtggt gactaacgag aaacaccgtg atatttgcga gaagaagtgg
    10861 aatatcccga gcggcaccat tccggcgaaa atcggtctgc acgcggtggc acaagaccgt
    10921 gcactgaaag acggcaagct gaatgtttac tggaccatgt gtaccaacaa catgcaggcc
    10981 gggccgaaca ttaatgaaga gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc
    11041 gtctccgatc cgtatccgac agtcagtgcg ctggcagccg acttgatcct gccgaccgca
    11101 atgtgggtag agaaagaggg cgcttacggt aacgccgaac gccgtactca gttctggcgt
    11161 cagcaggtac aggcgccagg cgaagcgaaa tcggatctct ggcagttagt ccagttctcc
    11221 cgccgcttca aaactgaaga agtatggccg gaagagctgc tggcgaagaa accggaactg
    11281 cgtggcaaaa cgctgtacga agttctgtat gccacccccg aagtgagcaa ataccctgta
    11341 tccgaactgg cggaagatca gctgaacgat gaatcccgcg agctgggctt ctatctgcaa
    11401 aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc acggtcacga tctggcaccg
    11461 ttcgatgact accacaaagc gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg
    11521 cagtggcgtt acagcgaagg taacgacccg tacgtgaaag cgggcgaagg ctataagttc
    11581 tacggtaaac cggatggcaa agcggtgatc ttcgcactgc cgttcgaacc ggcggcggaa
    11641 gcaccggatg aagagtacga cctgtggctc tctaccggac gcgttctgga gcactggcac
    11701 accggcagta tgactcgccg tgtgccggaa ctgcaccgcg ccttcccgga agcggtcttg
    11761 tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg
    11821 gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc gcggtcgtaa ccgtccgcca
    11881 cagggcctgg tgtacatgcc gttcttcgac gccgcacagc tggttaacaa actgacgctg
    11941 gatgcgaccg atccgctctc gaaagagacg gacttcaaga agtgcgcagt caaactggag
    12001 aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg
    12061 cgcgatgtcg ttcgcacagc aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa
    12121 cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac
    12181 gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc
    12241 ttaaaactgg cgacgctggc ctctggtctg tcggcgggca cgccatattt tgtcgcacgg
    12301 gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca aagtgtgccc aagcggtgcg
    12361 ctggatcgtg agattgaatc gatcgacgac gcgcggatgg ggctggcggt actggtggac
    12421 caggaaaact gtctcaactt tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg
    12481 aaaattgatg aggccatcac cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc
    12541 cgctttctgc cgacggttca cagcgacgcc tgtaccggtt gcggtaagtg cgaaaaagta
    12601 tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt cactggcgaa aggggagtta
    12661 ggtcaccatt accgcttcgg ttggctggag gggaacaatg gcaaatcgta aacgtgacgc
    12721 cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt caccgttggc tggtgttacg
    12781 tcgcctttgt cagttcttcg tgctggggat gtttttgagc ggtccatggt ttggtgtgtg
    12841 gatcttgcac ggcaactaca gcagtagcct gttattcgac accgttccgc tgaccgatcc
    12901 gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct gccacggtgg cgttgaccgg
    12961 tgcggtgatt atcaccgtgc tctacgccct ggcgggaaag cgattatttt gcagctgggt
    13021 ctgcccgctg aacccgatta ccgacttagc gaactggtta cgcaggcgct ttgacctcaa
    13081 tcagtctgcg acgatccctc gccacattcg ctacgtgctg ttggtggtca ttctgctggg
    13141 atcggcctta actggcacgc tcatctggga atggatcaac cccgtatctc tgatggggcg
    13201 cagcctggta atgggcttcg gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga
    13261 tctactggtc gttgaacacg gctggtgcgg gtacatttgt ccggttggcg cgctgtatgg
    13321 cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc gatcggcaga aatgtaaccg
    13381 ctgtatggat tgttttcatg tttgcccgga accgcatgtg ctacgtgccc cggtgctgga
    13441 tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga
    13501 tgtctgttct gaggatgtat ttacaataac tacacgatgg agttcgggag cgaaatcatg
    13561 aaaagccatg acctgaagaa agcgctgtgt caatggacgg cgatgctggc cctggtggta
    13621 agcggcgcgg tttgggcggc taatggcgtc gattttagcc agtcgccgga agtatccggg
    13681 acacaggaag gggccattcg gatgccaaaa gagcaggaac ggatgccgct gaactatgtg
    13741 aatcagccgc caatgatccc gcatagcgtt gaaggttatc aggtaacgac caataccaac
    13801 cgctgcttgc agtgccacgg tgtcgaaagt tatcgcacca ctggcgcgcc gcgtatcagt
    13861 cctactcact ttatggacag cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat
    13921 ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc caatcgtggg gaataccttt
    13981 accccatcaa aaggttacgg gaaataagag gttattatgg gaaattctga ccgtaagcct
    14041 ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca gccgtctggc gctggggacg
    14101 ctgctgttga tcggttttgt tggcggcatc gtcttctggg gtggctttaa caccgggatg
    14161 gaaaaagcca ataccgaaga gttctgcatt agctgccacg aaatgcgcaa cacggtgtat
    14221 caggaataca tggattccgt gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg
    14281 gattgtcacg ttccgcacga gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa
    14341 gagctgtatg gtaaaatttt tggcgttatt gacacgccgc agaaatttga agctcatcgt
    14401 ctgacgatgg cacagaatga gtggcggcgc atgaaggaca ataactcgca ggagtgccgt
    14461 aactgtcaca acttcgagta tatggataca accgcccaga aatcggttgc cgcgaagatg
    14521 catgaccagg cggtgaaaga tgggcaaacc tgtattgatt gccataaagg gatagcgcac
    14581 aagctgcccg atatgcgtga agtcgagcca ggtttttaac agggttattg cgtgggtatg
    14641 cttgaagcca gagagttact ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca
    14701 tttacgctga acgcaggaga gtgggtacaa atcaccggta gcaacggcgc ggggaagaca
    14761 acgcttctcc gtttgctgac ggggttgtct cgccctgacg caggcgaggt tctctggcaa
    14821 gggcagccct tgcatcaggt acgcgacagc taccatcaaa acctgttatg gataggccat
    14881 cagccgggga tcaaaacccg gctgacggcg ttagaaaatc tgcactttta tcatcgcgat
    14941 ggcgataccg cacaatgtct ggaagccctg gcgcaggccg ggcttgccgg attcgaagat
    15001 attcctgtaa atcagctctc ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg
    15061 ctgacccgtg ccacgttatg gatcctcgac gagcctttta ccgcgattga cgttaacggc
    15121 gtcgatcgtc tgacccagcg tatggcgcag catacggagc agggggggat tgtgattctg
    15181 actacccacc agccgctcaa cgttgctgaa agtaaaattc gccgcatttc actgacgcaa
    15241 acgagggccg catgatgttc tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc
    15301 atagcgccga aatcgccaac ccgctgtggt tcttcctgat tgtaattacc ctttttccgc
    15361 tcagtatcgg tccggagccg caactgctgg cgcgtattgc accgggcatt atctgggttg
    15421 ctgcactgct ttcatccttg ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg
    15481 gcagtcttga acaattgatg ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg
    15541 tgatggcgca ctggatggta agcggtctgc cgttactcat tctttcgcca ctggtagcaa
    15601 tgctactggg aatggatgtt tatggctggc aagtgatggc gctgacgctg ctgctgggaa
    15661 cgcctacgct tggctttctc ggtgcaccgg gcgtggcgct gacagtggga cttaagcgcg
    15721 gtggtgtgct gctcagcata ctggtgttac cgctgactat cccattactc atcttcgcca
    15781 ccgccgcgat ggacgcggct tccatgcatt tgcccgttga cgggtatctg gcaattttag
    15841 gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc gacggcggca gcgttacgaa
    15901 tcagcattca ataacgctgg attactgatt tttgttcgag tctggtatcg aaactatgtg
    15961 gaaaacactg catcaactgg cgatcccacc acggctgtat caaatctgtg gctggtttat
    16021 accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg
    16081 ctttgctccg gctgattatc agcagggaaa tagctaccgc attatctacc tgcatgtgcc
    16141 tgcggcgatc tggtcgatgg gcatttatgc atcaatggca gtggcagcgt ttattggcct
    16201 tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg atggccccca ttggtgccgt
    16261 gtttaccttt attgccctgg ttaccggctc tgcatgggga aaaccgatgt ggggcacctg
    16321 gtgggtatgg gatgcacgtc tgacttctga actggtgctg ctttttttgt atgtgggtgt
    16381 gattgccctg tggcacgcct tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct
    16441 ggtgctgatt ggcgtggtga atctgccgat tattcattac tccgtggagt ggtggaacac
    16501 cctgcatcag ggatcaacgc ggatgcagca aagtatcgat ccggcgatgc gttcgccgct
    16561 gcgctggtcg atttttggct tcctgctcct gtctgccacg ctgacgctga tgcggatgcg
    16621 taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg agtgaactga tactgaaaag
    16681 aggccgtaaa tgacccctgc atttgcttcc tggaatgaat ttttcgcaat gggcggttac
    16741 gccttttttg tctggctggc ggtggtgatg accgttattc cgctggtggt tttggtcgtg
    16801 cactcggtga tgcagcatcg cgcaattctg cgtggtgtgg cgcaacagcg ggcgcgtgag
    16861 gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa tattcgccgt aaaaaccgct
    16921 tgtggattgc ctgtgctgtt ctggcagggt tggcactgac tatcggtctg gtgctatatg
    16981 cgctgcgctc gaatatcgat ctcttttata cgccggggga aattctctac ggcaagcgtg
    17041 aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg
    17101 gtagcgtgca gcgcgatccc aattcgctaa aagtaacttt caccatttac gacgctgaag
    17161 gttcagtaga tgtctcttac gaaggcattt tgccggatct gttccgtgaa gggcagggcg
    17221 ttgtggtgca gggcgaactg gaaaaaggca atcatatcct cgcaaaagaa gtgctggcga
    17281 aacacgacga aaactacacg ccgccagaag ttgagaaagc gatggaagcc aatcaccgtc
    17341 gcccggcgag tgtttataag gacccagcat catgatgccg gaaattggta acgggctgct
    17401 gtgtctggcg ctaggaattg cgctgctgct gtccgtgtat ccgctatggg gcgtagcgcg
    17461 cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt
    17521 ggctggcgca tttctggtac tggtcaatgc cttcgtggtc aacgacttca ccgtcaccta
    17581 tgttgccagc aactccaata cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg
    17641 cgcgcatgaa ggctcgctcc tgctgtgggt gctgctgatg agcggctgga cctttgcggt
    17701 agcgattttt agtcagcgta ttccgctgga tattgtggcc cgcgtactgg cgataatggg
    17761 gatggtcagc gtcggctttt tgctgttcat tctctttacc tctaacccgt tctcacgcac
    17821 gttgccgaac ttcccgattg aagggcgcga tcttaacccg ctgttacagg atccggggct
    17881 gatcttccat ccgcctctgc tctatatggg gtacgtgggg ttctcggtgg cgtttgcttt
    17941 tgccattgct tctttgttgt gcgggcgtct ggacagcact tatgcgcgtt ttactcgtcc
    18001 gtggacgctg gcggcgtgga tcttcttgac gctcggcatt gtgctcggtt ccgcatgggc
    18061 ctattacgaa ctcggctggg gcggctggtg gttctgggat ccggtagaaa acgcctcgtt
    18121 tatgccgtgg ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc
    18181 cagcttcaaa gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctgctggg
    18241 gactttcctg gtgcgttccg gcgtgctggt atcggtacac gcgtttgcct ctgatccggc
    18301 acgcggtatg tttatcctcg cctttatggt actggtgatt ggtggttcgc tgctgctgtt
    18361 tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc
    18421 tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg ctggtggtat tactggggac
    18481 gctgctgccg ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt
    18541 cttcaacacc atgtttacct ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc
    18601 tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc aatttattga ttatcgcctt
    18661 catctcaacg ctggtgctgt cgctgctgct gccgtggctg ttcgaaagca aagttgtggc
    18721 gatgacggtg ctcggcctgg caatggcctg ctggattgct gtgctggcaa ttgcggaagc
    18781 tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt tattggggaa tggtggcggc
    18841 tcacctgggg ctggcagtga caattgttgg tattgccttt agccagaact atagcgttga
    18901 gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt catgaatatc gcttcacctt
    18961 ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt
    19021 aacgcgcgat ggcaagccgg aaacggtgct gtatgcggaa aaacgttatt acaacactgc
    19081 cgggtcgatg atgaccgaag cggcaattga cggcggcatc acgcgtgacc tgtacgccgc
    19141 gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt ctttactaca aaccatttgt
    19201 tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc ggactactgt gtctgtttga
    19261 tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg ccggaggccg tatgaagcgc
    19321 aaagtattgt taattccgtt gattatcttc ctggcgattg ccgcggcgct gctgtggcag
    19381 ctggcgcgta atgccgaagg ggatgatccg accaatctgg aatcggcgct cattggcaag
    19441 cctgtgccga agtttcgtct cgaatcactg gacaatccgg ggcagtttta tcaggcggat
    19501 gtgctgactc agggcaaacc agtactgctt aacgtctggg cgacctggtg tccgacctgc
    19561 cgtgcggaac atcaatatct gaatcagctt tcagcgcagg gcattcgcgt ggtcggcatg
    19621 aactataaag acgatcgcca gaaggcaatc agctggctga aagagctggg caatccttac
    19681 gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg atctcggtgt ctatggcgcg
    19741 ccagaaacgt tccttattga cggcaacggc atcattcgct atcgccatgc gggcgatctc
    19801 aatcctcgcg tctgggaaga agagatcaag ccgctgtggg agaaatacag taaggaggcc
    19861 gcacaatgag gtttttattg ggcgtgctga tgctgatgat ctccggctca gcgctggcga
    19921 ccatcgacgt gttgcagttt aaagatgaag cacaggaaca acagttccgt cagctcactg
    19981 aagaactgcg ctgcccgaaa tgccagaata acagcattgc cgattccaac tcgatgattg
    20041 ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga aggtaaaagt aagaaagaga
    20101 ttgtcgatta tatggtggcg cgttacggca acttcgtcac ttacgatccg ccgttaacgc
    20161 cgctgaccgt gctgctgtgg gtgctgccag tagtggctat tggcattggc ggttgggtca
    20221 tttacgcccg ctcgcggcgt cgggtacgcg tggtgccgga agcgtttcct gaacaaagcg
    20281 tgccggaagg taagcgtgcc ggatatgtcg cttatttgcc gggcattgtg gtggcgttaa
    20341 ttgtggctgg cgtcagctac taccagaccg gaaattatca acaggtgaaa atctggcagc
    20401 aggccacggc acaggctccg gcgttgctgg acagggcgct ggatccgaaa gccgatccgc
    20461 tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg tactcaactg caaaaaaatc
    20521 cgggagatat agaaggctgg attatgttgg gccgcgttgg catggcgctg ggtaacgcta
    20581 gtatcgccac cgatgcatac gctactgcgt atcgcctcga tccgaaaaac agcgatgccg
    20641 cactgggtta tgctgaagcg ttgacacgtt catctgatcc caacgacaac cgcctcggcg
    20701 gcgaactgct gcgccagttg gtgagaagcg accacagcaa tatccgtgtg ttaagcatgt
    20761 atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc cgttgccgcg tgggagatga
    20821 tgttgaaact cttacctgcc aacgatactc gccgtgcggt gattgaacgt agtatcgcgc
    20881 aggcgatgca acatttgtcg ccgcaggaga ataaataaaa agaggcccga tggtgatgcc
    20941 atcgggctat ttttattgtg ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg
    21001 tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt acttttactg tctgctctga
    21061 aatattcaac accgaggcaa tctgtttatt tgacagcccc tgtgccagct cgtgcagaac
    21121 atccagctcg cgctccgtca gcacgctgaa gggatcttct tccgcgccaa acatttcacg
    21181 ttcacgtaag tactgattga cgcgttcgct aaagactttg ctgcctttcg ctccagcacg
    21241 aattgcttcc agcaatactt ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc
    21301 gtctatcagc gcaaagacat cgctggaggc atctgatacg gtcaggataa taatttgcgc
    21361 ggtaacgcca tccctgcgca aggcattgag ggtatccagg ccactcatac ctttcatatt
    21421 gagatccagc aagatcacgt cgatatccag tctattcgcc agatcgatcg cgctcgcgcc
    21481 gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc tccagtaact gacgaacacc
    21541 gcgtcgcata agtggatgat catccacaat catcacctga aaaggcgttg cttcaggcat
    21601 agtagtctcc tgaggtttta ttagaatcat tatgtttgag cgtagcgttg gtaacgctac
    21661 caggcgcgtg aatgttaccc cattttatgt atggacatgt agtgaaaact acaaccttgt
    21721 cgttcgcttt ggcatcattt tcattataaa gacggaattt tgcatcagct tctcatcaag
    21781 aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc tttaaacagt ttttaaaaac
    21841 tgactcccgg tatggagcta tgggtatttt ctgtacccaa tgcttttaac agcaattaat
    21901 ttcataggat gaaagctcaa tgcatcaatc tggttctgtt tctctttgtc gttccgcaat
    21961 atctgttctg gtggctacag cgttatattc acccatagca ttggcatcaa cagttgagta
    22021 tggtgagaca gttgatggtg ttgtcctgga aaaagatatc cagctggttt atgggaccgc
    22081 caataatacg aaaatcaatc ctggcggaga acagcatata aaagaatttg gtgtaagtaa
    22141 taatactgaa attaacggag ggtatcagta cattgaaatg aatggcgccg cagaatactc
    22201 agtattaaat gacggttatc aaattgttca aatgggtggc gcggcaaacc agactacgct
    22261 caataatggt gtgctacagg tttatggcgc agcgaatgat accacgatta aaggcgggcg
    22321 cttaatcgtt gaaaaagatg ggggggccgt ctttgtcgct atcgaaaagg gaggactact
    22381 ggaggttaaa gaggggggat tagcgattgc ggtagatcag aaagcaggcg gtgctattaa
    22441 aacaaccacg cgggccatgg aggtattcgg aacaaaccgt ctcggtcagt tcgatatcaa
    22501 gaatggtatt gctaataata tgttgttgga aaacggcgga agtttgcgag ttgaagaaaa
    22561 tgacttcgct tataatacca ctgtagatag tggcggctta ctggaggtta tggatggcgg
    22621 gactgtaact ggcgttgata aaaaagcagg cggaaaatta attgtctcaa cgaatgcgct
    22681 ggaagtgagt ggtccaaaca gtaaaggcca atttagtata aaagatggtg tgtcaaaaaa
    22741 ttatgaactg gatgatggtt ccgggctcat tgttatggag gacacgcagg ccattgatac
    22801 tatccttgat gagcatgcca ctatgcaatc gctgggaaag gatactggta cgaaagtgca
    22861 ggcaaatgcg gtatatgatc tcggtcgatc atatcagaat ggaagtatca cgtattcctc
    22921 aaaagccatc tctgaaaata tggttatcaa caatggccgc gctaacgtct gggctggcac
    22981 aatggttaac gtttcagtca gagggaatga tggcattctt gaggtcatga agccgcaaat
    23041 aaattatgca cccgcaatgt tggtgggtaa ggtagtggtt tctgagggcg cttcttttag
    23101 aacgcatagt gccgtggata ccagcaaagc ggacgtttcg ctcgaaaata gcgtatggac
    23161 catcattgcc gatatcacta cgacgaacca aaacaccctc ctcaacttag ccaaccttgc
    23221 gatgtctgac gcaaatgtga ttatgatgga tgagccagtg actcgttcat cagtgacggc
    23281 aagtgcggaa aatttcatta cgttgaccac caataccctg tcgggaaacg gcaattttta
    23341 tatgcgtacc gatatggcta atcatcagag cgatcagctc aacgtcaccg gtcaggcaac
    23401 aggtgatttc aaaatattcg tgacggacac cggtgccagc ccggcagcag gagatagcct
    23461 tacactggta acaacgggcg gcggtgatgc tgcatttacg ttgggcaatg ccggaggcgt
    23521 tgttgatatc ggtacgtatg aatatacctt gctggataat ggcaaccata gctggagttt
    23581 ggcagagaat cgcgcgcaaa ttaccccttc aaccactgat gtgctgaata tggcggcagc
    23641 acaaccgctg gtatttgatg cagaactgga caccgtgcgt gagcgtcttg gtagcgtaaa
    23701 aggcgttaat tacgatacgg cgatgtggag ttcggcaatt aacacccgca acaacgtgac
    23761 cactgatgcg ggcgctggct ttgagcaaac attgaccggg ctgacgctcg gtatcgatag
    23821 ccgtttctcc cgtgaagaaa gcagcacaat tcgcggcttg ttctttggtt actctcattc
    23881 tgatattggt tttgatcgcg gcggcaaagg caatgtcgat agctataccc tgggggctta
    23941 tgccggttgg gagcatcaga acggtgccta tgttgatgga gtggtgaaag ttgaccgttt
    24001 tgccaacacc atccatggca agatgagtaa tggggcaaca gcgtttggcg attacaatag
    24061 taacggcgcg ggtgctcatg tcgagagcgg gttccgttgg gttgacggat tgtggagtgt
    24121 tagaccctat ctggccttta ccggctttac cacagatggt caggactaca cgttatcaaa
    24181 cggcatgcgc gcggatgtgg gaaatacccg gatattacgc gctgaagcgg gaacggcggt
    24241 aagctatcac atggacctgc aaaacggtac gacgctggaa ccctgactga aagccgccgt
    24301 gcgtcaggaa tacgccgatt ctaaccaggt gaaagttaat gacgatggca aatttaataa
    24361 tgatgtggct ggaacccgtg gcgtttatca ggctgggata aggtcatcgt ttaccccgac
    24421 gttaagcggt catttgtcag tcagctatgg caatggcgca ggggtagaat cgccgtggaa
    24481 tactcaggcg ggtgtggtct ggacgttctg ataacaaaaa ataaacaggc tgtgatgtgt
    24541 cacggtctgt ttatcgaatt aattgcagat ataaaaaaaa ccaaccgtaa aggttggttt
    24601 tttcttggga tttttggcac gagagtagtt taattgagta tgtaactaca tgattttaaa
    24661 tagtatgctt gattcaactt ttaaaccgta taactaaacg tataactaaa atcatttgct
    24721 gaaaaagtgt acaatttaaa tgcgagtttt cctgtggtaa aagctccaac cgtaacgatg
    24781 ttcgttatgg ttgccttgat ggtctgcgtg gtcattatga ccattcagga tttgaagggt
    24841 gacgcttccg acttcctcaa attgaggttt ccgaaaatat cagcgggtta gcgattaatc
    24901 tgaccattaa ttaaactgcg aaaatttcgt aattatcccg atatggggat caccatatcg
    24961 ggattgatgc cggaaaaatc agcttttcgc cagaacggtg acggccttat cgagtttttg
    25021 ccatccgcaa taggtgattg ccctgaacgc tgtagcgtca ttctggaaaa ggctaaccgt
    25081 ccctgattcg gtttttatta ccgcctcatc agttggggtt ataaccgtgg cttcaacgtt
    25141 aataatcagc ttttgagcgt ctatcagcat catgcgatcc gcctgttcgc tcacaattac
    25201 cggtattctc aaaagctcta tctgccagaa ctcagcatca gtaagctgta aggcgttttc
    25261 tacgggcaag attagcgacg acgtggcaag cgtgttaccg ttcgctatgt gctttttgaa
    25321 aagctctatg aagttttcgg cctcatcagc accattaagc acgccctcag gggatacgcc
    25381 cgccacctcc tggtcactgg aaagaaattt tttatctatt tctttcaccg attccgatgt
    25441 gatcaagtct ctgatcgctg aattggtacc aggggtgcta aatttgatta gctcgttatt
    25501 cagcatcaca atagcgccaa gtttgcacag ttttagcgca ctttcgttta cttctccctc
    25561 tttgacaggg atttcttcgg cttctttaag ccatttacta cccttgcctg atattgactg
    25621 caattttgcg ccaaacggaa gattcagcgc gctgctgatt agcttgcctg gtacagactg
    25681 cgaaaaaata tactcgccaa gctctcttac tatcggctct gccaccagcg acggcatatt
    25741 atccgtagtc attccggcaa caatggattt ttcaagaaat ttcgatactg ccggatcatt
    25801 ttttttcgtc atttcaattg cgtctgcatg gctgaattta cacgatgtaa gcacgctggc
    25861 ggcgcgaact gccgacacgc ctttcatgtt tttagaaatc attatcttct tcctcctggt
    25921 aacatggggt tagggtttcg tgaaaatatg gcatcagaac agcggcagcc tgtaaccgta
    25981 tttcggttgg ctgctgcttg tcgttcatga tttccgtgag tacatcaata gggtctgtga
    26041 tgtattcgct catagttcgc tacctcagtg ttttaagcca gttggtggct tagggatggg
    26101 gaaccgattt gaggagacat tagcggcatt gagtttttta acctcttttt tcccaatgct
    26161 gccgatcttg cggtgggtga actgggccag cttaaacgcg gcatctaaag ccagttttgg
    26221 atctgtgtcc atgttgtcaa tcagtatcct gcccattgct ttgatggggt cgggtaggcc
    26281 gttatccatc atctcaatgg tgttaattga tgcccgttcc ggtgtaactt tttcggtaac
    26341 tttctttgta acttttttcg gttttgtaac ctgaaaagtt acatcagaag ttacagcctt
    26401 aagatacttt ttcacctccg gatcattgga tagctgccag cctttaaccc ttgcagtctt
    26461 ttcgctgtat cccgcatgta tcgcggcttc cgttggattt gtgccctttg ataacgcatc
    26521 agcaaaccgc ttttttttgg tcgttaatgc catgcaaaaa cctcgttttg agggggtgta
    26581 acttttcgtg taactttttt gaaaagcaaa attttctatg aatgagactg ggggcggtgt
    26641 ccagggcgat cgactttccg gccggaacac tccccccctg tcattgctca ccgagtaacg
    26701 agatcaggcc ttcaacttca tcactcagct tgtttattcg tgtctcaagc tctgcgatct
    26761 tttccatcaa tggcgctatg gtgtctcttg tttcctcgcc aatggcttta atgatgcttg
    26821 cctcgcgttc tgtgatagcc attgtttacg cctcacttgt tgcaatgccc aggcgttcgc
    26881 gtaacagtac gcgcgcaaga tgccctattg ttggtgttgc cgccctgccg tagatacctg
    26941 attcattcat ccggcgcaca atatcgaact gcaacttttt aagctcgtta aggatttcat
    27001 gatcaatcat cacggtagcg ctttgcttta tctcttccac tttgttttct cctgatactt
    27061 atcattgcat aaccagtatt ttcgatagat tatcattaat taaccatctt tgaagatata
    27121 tgagatcgaa aaaaagccgg attgctccgg ctatgtgctc acttcttcgg ttattctcca
    27181 gcgcttttaa cgttgatggt tgttacctgt tccgcttcgg caatctcccg ttctgtcagc
    27241 gtggcaaagt ttgccgccgc tgtggtcatg aatgcgctta tcagttcggg atgctccttc
    27301 gcgtatcctt cccccgcgtg gcggtctatc gttctgattg ccacctttaa ggaaagctct
    27361 gtcatgtcta acgctttata ttttggcgct gtcttatctc tggtttttct gttcattccc
    27421 cactactccc cacttttgct ccccactttt tgaaattacc caccgtctcc ccacctcttt
    27481 tttttgtgat tctaacgtta tgtttttcaa tatgtttttt actccccact tttttgggta
    27541 tatacaggtg ggaaagtggg taattatgtt tcaattttgt taaattcccc actcccccca
    27601 cttttactcc ccacttttta cagcgggcgc acgtcatcac cgtcaataac gattacgcca
    27661 tcattttcca gcttgtacag ccagcgccgg aagtgcttca tctcataccc cagctttttc
    27721 atatcgtcgc ggagaagggc gatagtgcac gcctccccgt gtgctgttcg tgttctgatg
    27781 cactgccata gcgcggcgtg attctccgtc ttgttccctg cctcctcgat gcgctccagt
    27841 tcaacgggag ggcgcggctt atccaccacc accagcgacg tgattaactc cccgtcagcg
    27901 tcggtaaaaa gctccaccac gcgtaagtca tatgctgctt ctttgagttc ctccgcgtcc
    27961 ttcattttgg tacatgagat aaccagcgct tcgctgcctg cgtcctccct gcgtatccgg
    28021 tattcggcgt ccagtgatgc acgaaatgca ctggaaccac gcgccccctt tgtttcatcc
    28081 ttgccggaat ggtgaaccac cagcaccgtt gcccctgtgc gccgtttcag ttcgtcacac
    28141 ccacggataa acgcccccat atcacgggaa tcattttcat cattgccgcc aaagcagcgc
    28201 gccagcgtat ccagaataat cattcgaacc ggtttacccg tttcccgttc cacctgacgg
    28261 gcagcgataa ccatttcctc aacatcaagc ggggcagccg gaaagatggg acggtttacc
    28321 agatacagat ttttcacctg ctcaccgtgc acaacctccc aggcttttac acgacgcgga
    28381 acgccgatac cgccttcacc aaccacatag agaacagcgc catgcgccac cctgcggcct
    28441 ccccactggc ggcccgtgga aacgtgacac gcccacgaac ccgcaaggaa cgatttatat
    28501 gaaccgctcg ccccgtatat gctgcacagc gataccgccg gaataatccc cttaaccacg
    28561 taatcaagtt gtgtgtcgta tccggcagat cccacgctca ttggtagcgt ggtttttcgc
    28621 tggtggtgtt tttcttcccc tggctttttc ccccgctccc gttccagata ttcgcgccag
    28681 ttctcccgct t
//
LOCUS       DAZKGS010000051        28643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKGS010000051 DAZKGS010000000
VERSION     DAZKGS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..440
                     /locus_tag="ACHOQY_002127"
     CDS             <1..440
                     /locus_tag="ACHOQY_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9678221.1"
                     /translation="VEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQP
                     VFASLNDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGER
                     DSKFRALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            455..1312
                     /gene="menB"
                     /locus_tag="ACHOQY_002128"
     CDS             455..1312
                     /gene="menB"
                     /locus_tag="ACHOQY_002128"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9678222.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1312..2274
                     /gene="menC"
                     /locus_tag="ACHOQY_002129"
     CDS             1312..2274
                     /gene="menC"
                     /locus_tag="ACHOQY_002129"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9678223.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2271..3626
                     /gene="menE"
                     /locus_tag="ACHOQY_002130"
     CDS             2271..3626
                     /gene="menE"
                     /locus_tag="ACHOQY_002130"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9678224.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3736..4002
                     /gene="pmrD"
                     /locus_tag="ACHOQY_002131"
     CDS             3736..4002
                     /gene="pmrD"
                     /locus_tag="ACHOQY_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9678225.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3996..4382)
                     /gene="arnF"
                     /locus_tag="ACHOQY_002132"
     CDS             complement(3996..4382)
                     /gene="arnF"
                     /locus_tag="ACHOQY_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9678226.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4382..4717)
                     /gene="arnE"
                     /locus_tag="ACHOQY_002133"
     CDS             complement(4382..4717)
                     /gene="arnE"
                     /locus_tag="ACHOQY_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9678227.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4714..6366)
                     /gene="arnT"
                     /locus_tag="ACHOQY_002134"
     CDS             complement(4714..6366)
                     /gene="arnT"
                     /locus_tag="ACHOQY_002134"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9678228.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6366..7256)
                     /gene="arnD"
                     /locus_tag="ACHOQY_002135"
     CDS             complement(6366..7256)
                     /gene="arnD"
                     /locus_tag="ACHOQY_002135"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9678229.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7253..9235)
                     /gene="arnA"
                     /locus_tag="ACHOQY_002136"
     CDS             complement(7253..9235)
                     /gene="arnA"
                     /locus_tag="ACHOQY_002136"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9678230.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9235..10203)
                     /gene="arnC"
                     /locus_tag="ACHOQY_002137"
     CDS             complement(9235..10203)
                     /gene="arnC"
                     /locus_tag="ACHOQY_002137"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9678231.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10207..11346)
                     /gene="arnB"
                     /locus_tag="ACHOQY_002138"
     CDS             complement(10207..11346)
                     /gene="arnB"
                     /locus_tag="ACHOQY_002138"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9678232.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11654..12256
                     /gene="ais"
                     /locus_tag="ACHOQY_002139"
     CDS             11654..12256
                     /gene="ais"
                     /locus_tag="ACHOQY_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9678233.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(12295..12720)
                     /gene="nudI"
                     /locus_tag="ACHOQY_002140"
     CDS             complement(12295..12720)
                     /gene="nudI"
                     /locus_tag="ACHOQY_002140"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9678234.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            12999..13541
                     /gene="yfaZ"
                     /locus_tag="ACHOQY_002141"
     CDS             12999..13541
                     /gene="yfaZ"
                     /locus_tag="ACHOQY_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9678235.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            13641..14843
                     /gene="yfaY"
                     /locus_tag="ACHOQY_002142"
     CDS             13641..14843
                     /gene="yfaY"
                     /locus_tag="ACHOQY_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9678236.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            14907..15368
                     /locus_tag="ACHOQY_002143"
                     /pseudo
     CDS             14907..15368
                     /locus_tag="ACHOQY_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(15409..16311)
                     /gene="rpnE"
                     /locus_tag="ACHOQY_002144"
     CDS             complement(15409..16311)
                     /gene="rpnE"
                     /locus_tag="ACHOQY_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9678237.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(16505..17695)
                     /gene="glpC"
                     /locus_tag="ACHOQY_002145"
     CDS             complement(16505..17695)
                     /gene="glpC"
                     /locus_tag="ACHOQY_002145"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9678238.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(17692..18951)
                     /gene="glpB"
                     /locus_tag="ACHOQY_002146"
     CDS             complement(17692..18951)
                     /gene="glpB"
                     /locus_tag="ACHOQY_002146"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9678239.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(18941..20569)
                     /gene="glpA"
                     /locus_tag="ACHOQY_002147"
     CDS             complement(18941..20569)
                     /gene="glpA"
                     /locus_tag="ACHOQY_002147"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9678240.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            20842..22200
                     /gene="glpT"
                     /locus_tag="ACHOQY_002148"
     CDS             20842..22200
                     /gene="glpT"
                     /locus_tag="ACHOQY_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9678241.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            22205..23281
                     /gene="glpQ"
                     /locus_tag="ACHOQY_002149"
     CDS             22205..23281
                     /gene="glpQ"
                     /locus_tag="ACHOQY_002149"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9678242.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(23323..23562)
                     /locus_tag="ACHOQY_002150"
                     /pseudo
     CDS             complement(23323..23562)
                     /locus_tag="ACHOQY_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            23560..23664
                     /locus_tag="ACHOQY_002151"
                     /pseudo
     CDS             23560..23664
                     /locus_tag="ACHOQY_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23744..24394
                     /gene="inaA"
                     /locus_tag="ACHOQY_002152"
     CDS             23744..24394
                     /gene="inaA"
                     /locus_tag="ACHOQY_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9678243.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(24448..24702)
                     /gene="yfaE"
                     /locus_tag="ACHOQY_002153"
     CDS             complement(24448..24702)
                     /gene="yfaE"
                     /locus_tag="ACHOQY_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9678244.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(24702..25832)
                     /gene="nrdB"
                     /locus_tag="ACHOQY_002154"
     CDS             complement(24702..25832)
                     /gene="nrdB"
                     /locus_tag="ACHOQY_002154"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9678245.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(25921..28206)
                     /gene="nrdA"
                     /locus_tag="ACHOQY_002155"
     CDS             complement(25921..28206)
                     /gene="nrdA"
                     /locus_tag="ACHOQY_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9678246.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            28334..28477
                     /gene="ypaB"
                     /locus_tag="ACHOQY_002156"
     CDS             28334..28477
                     /gene="ypaB"
                     /locus_tag="ACHOQY_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP9678247.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         6859 a         7156 c         7654 g         6974 t
ORIGIN      
        1 ttgtcgaagg cgggcatccg gggctgcaaa atgctgaaca acgtgcggaa cgtcagcgtt
       61 ccgatcgcca atgggcgcag cgtttttgca cagaaccgtt aacggcggta tttgccgact
      121 ggtatcaaca gcctgttttt gcctcgctca atgacgatca acgccgggag ctggtggcgc
      181 tgcgcagcaa caataatggc gcaacccttg ccgccatgct ggaggcgact tctctcgccg
      241 tccagcctga tttacgtgct aaccttagcg cccgcacatt tgcgttttat tatttatgtg
      301 gtgaacgtga cagcaaattc cgcgccctgg cggcggaact ggctgccgac tgccatgtca
      361 ttcctcgcgc cggacataac gcgcatcggg aaaatcccgc tggcgtaatc gcaagtctgg
      421 cgcagatctt gcgtttctga ctaaaggaca caatatgatt tatcctgatg aagcaatgct
      481 ttacgcaccg gttgaatggc acgactgctc cgaaggtttc gaggacattc gttatgaaaa
      541 atccaccgac ggtatcgcaa aaatcaccat taatcgtccg caggtgcgca atgccttccg
      601 tcctctgacg gtaaaagaga tgatccaggc actggcagat gcgcgttatg acgacaatat
      661 cggcgtgatc attctgactg gcgcaggcga taaagcgttc tgctccggtg gtgaccagaa
      721 agtgcgtggt gattacggcg gctataaaga tgattccggc gtacatcact tgaacgtgct
      781 ggacttccag cgtcagatcc gtacctgtcc gaaaccggtt gtcgcgatgg tggctggcta
      841 ctccatcggc ggtggtcacg ttctgcacat gatgtgcgac ctgactatcg cagcagataa
      901 tgccatcttc ggtcagactg gcccgaaagt cggttccttc gacggcggct ggggcgcttc
      961 ctacatggct cgtatcgtcg ggcagaaaaa agcgcgagaa atctggttcc tgtgccgtca
     1021 gtacgacgca aaacaggcgc tggatatggg ccttgtcaac accgtggtac cgctggcgga
     1081 tctggaaaaa gaaaccgtcc gttggtgccg cgaaatgctg caaaacagcc cgatggcgct
     1141 gcgctgcctg aaagctgcgc tgaacgccga ctgtgacggg caggcggggc tgcaggagct
     1201 ggcgggcaac gccaccatgc tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc
     1261 tttcaaccag aaacgtcagc ctgatttcag caaatttaaa cggaatccgt aatgcgtagc
     1321 gcgcaggtat accgctggca gatccccatg gacgcggggg tggttctgcg cgacaggcgg
     1381 ttaaaaaccc gcgacgggct gtacgtttgc ctgcgtgagg gcgagcgcga agggtggggg
     1441 gagatctccc cactgccggg cttcagccag gaaacctggg aagaggcgca aagcgtgctg
     1501 cttgcctggg taaataactg gctggcaggc gattgcgagc taccgcagat gccttccgtt
     1561 gcctttggcg taagctgtgc attggcggag ctggcagata cgctcccgca ggcggccaat
     1621 taccgtgcgg caccactgtg taatggcgat cctgacgatc tgatcctcaa acttgctgat
     1681 atgccaggcg agaaagtggc gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc
     1741 atggtggtta atctgttgct ggaggcaatt ccggatctgc atttgcgtct tgacgcaaat
     1801 cgcgcctgga caccgctgaa aggccagcag tttgccaaat atgttaaccc ggattatcgc
     1861 caccgcatcg cgtttctcga agagccgtgc aaaacccgcg atgattcgcg agcgtttgcc
     1921 cgtgaaaccg gcattgccat tgcctgggat gaaagcttgc gcgagccgga ttttgccttt
     1981 gtggctgaag agggcgtgcg cgcggtagtt atcaaaccca cgctcacggg cagtctggag
     2041 aaagtacgcg agcaggtaca ggcggcgcac gcgctggggc tgacggcggt gatcagttct
     2101 tccattgaat cgagcttagg cttaacgcaa ctggcgcgga ttgccgcctg gttaacgccg
     2161 gacaccattc cagggctgga cacgctggat ctgatgcagg cgcagcaggt acgtcgctgg
     2221 ccgggtagca cgctgcctgt cgtggaagtt gatgcactgg agcggttgtt atgatcttct
     2281 ctgactggcc gtggcgtcac tggcggcaag tgcggggaga agccatcgcc ttacgtctta
     2341 atgacgagca acttaactgg cgcgagcttt gtgctcgcgt cgatgaatta gcctcaggct
     2401 ttgcggcgca gggcgttgtt gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc
     2461 aaacgctgct cgcctggctg gcgttactgc aatgcggggc gcgggtgttg cccgtgaacc
     2521 ctcagctgcc gcaaccgttg cttgaagaat tgctgcccaa tctgacgctg caatttgcgc
     2581 tggtgccgga tggggaaaac acgtttccgg cgttaacgtc gctgcacatt cagcgggttg
     2641 aaggcgcaca tgccgctacg tggcagccga cgcgtctgtg ctcaatgacg ttgacctcgg
     2701 gttctaccgg gttgccgaaa gccgctgtcc atacttatca ggcccatctt gccagtgccg
     2761 aaggggtgtt atcgttgatt ccgtttggcg atcacgatga ctggttgctc tctttaccgc
     2821 tgtttcacgt ctccggtcag ggaattatgt ggcgctggtt atacgctggt gcgcggatga
     2881 cggtgcgtga taaacagcca ttggagcaaa tgctggcagg ctgtactcat gcttcactgg
     2941 tgccaacaca actctggcgt ttgctggtta accgcagttc cgtttccctg aaagcggtgt
     3001 tacttggcgg cgcggctatc ccggtcgagt tgacggaaca ggcgcgcgag caggggattc
     3061 gttgcttttg cggctatggt ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg
     3121 acggcctggc agacgttggt tcgccgctgc cgggtcggga agtgaaaatc gttaataatg
     3181 aagtgtggct gcgggctgcc agtatggcag aaggttactg gcgtaacggg caactggttt
     3241 cactggttaa tgacgaaggc tggtacgcta cgcgcgatcg cggtgagatg cataatggca
     3301 agctgaccat tgtcggacgt ttagacaatc tattcttcag tggcggagag ggtattcagc
     3361 cggaagaagt cgagcgtgtt attgctgcac atcctgcggt tttgcaggtg tttatcgttc
     3421 ccgttgccga caaggagttt ggtcatcggc cggtagcggt ggtggagtat gaccagcaaa
     3481 ccgttgatct tggcgaatgg gtgaaagata agctggctcg ttttcaacaa ccggtgcgct
     3541 ggctgacttt accgccggag ctgaaaaacg gcggtattaa aatttcacgt caggcgctaa
     3601 aagagtgggt gcaacgtcag caataattaa ccctccgttt cacttctcat ttctgccatt
     3661 ccattgccgt tgataaagag tttatctgtt aatctgtaaa ttgatgtgaa aactcttagc
     3721 aaacaggata atgcaatgga atggctggtc aaaaaatcgt gctgcaataa acaagataac
     3781 agacatgtac ttatgctctg cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag
     3841 agtgacttcg ccgtgaaagt gggggattta ctctcgcctt tgcagaatgc gctttattgt
     3901 attaatcgtg aaaagctgca tacggtgaaa gtcctctcgg caagcagtta tagccccgat
     3961 gagtgggaac ggcagtgtac agcggcaggg aaaactcagt aacgttgttt tgtcgtgggc
     4021 aggaaaatca gcatcaaccc gctcataata caggctactc ccagtagtgc tttcagcgaa
     4081 aaagtccctt cccagccggg aagtaccatt gaagcaatcc ataccagcac ataactcata
     4141 ctgagcaagg cataggcttt gcttagcgca agtttatgta gcgttttata ccaacaaaaa
     4201 acggaaagca gataacccag cagcccgagt aacagtatcc gggcatccag gccaaaagct
     4261 aacagagccg cgataaaatc ccataagtgc gtcatcggcg gcagatgact tgccgcaaaa
     4321 cccaggctta attgcgcaac tgaggcaata atgacgctga ataatcccca tatcaggccc
     4381 attacaccgt actcccgagg atcacaatgc caccaataat gaacgccacc ccacaccagt
     4441 gacgcggcga taccggttcg tgccacagtt ttactgcagc cagcgtcacc cagacaaaat
     4501 tcagacttaa catcgggtag gcaatgccta ccggtacgtt ctgcaagacc agcagccaga
     4561 gcaccatggc aagaccaaga caagccagcg ccagtcccag ccacagcacg atatgtttgc
     4621 gccgtttgtt tatcgccaca aagcaggttg cctgtttctg acacaactgc ccggcaacgc
     4681 taagcaagct ggcaaagact aatgttagcc agatcatttg ggacgatact gaatcagcac
     4741 cagacgctcc tgacgatcga tggcatctgc gggcggaatg gcgagactgt tgatatcttc
     4801 atcgcggtca accgaaagca cgagagtaat aatcccctcc tgacgatgtt gattaagcca
     4861 gtttgcgaac tcatcaccgc tgacaaatct ccctttcgca tccggataat taaggccgta
     4921 tttcaactca cctgtctggc gatacatgat gatgtcatcg cgttgcaggc tccatgccag
     4981 acctgcggca acaccgacgc tatcagtaag aatatagcgg cttggctgaa gtgattcttg
     5041 tgtcatctcg acaaaaaatt gcggatgttt tccttccata actctgtcag gaattgaaaa
     5101 tcctaccagc aacgccagcc ccagcgggca aagcgcggca aaaggccagg tcttttcgac
     5161 gtttgttaag gtgtaccagc cgaaaaatgc ccatagcgaa aaaatcgacc aggcacaaaa
     5221 gactttatag ctctcgaagg tttgccacac cggcgtgttc attggtcccc acggggagac
     5281 cacaaatgtg gcaataatgc cagtgacgcc aaaagcgatg ttaatccagc cattaatccg
     5341 cagcgccaga ggattatttt ttgctgccag caaagcgtaa tgcgccatca gcattgccag
     5401 agatgcaaag caggaaagaa tataggtggg caatttacct ttagcgacgg agaaaaacag
     5461 cagcggcatt atcgtccagc tcaacaaata gacggttgcg gaatgcttgc ggtttttcca
     5521 gcctgtgtac agtgcaccgg ggagtaatcc cagccacggc aggctaccgg caatgatgac
     5581 cggcacgtag taccagaacg gagctctatg ttgggcatca tccagtgcaa agcgttgaat
     5641 atgctcaacc cagaaaaaat agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc
     5701 ccaagggaga accgtcagta cgcaactgat aaccgccagc cagccgtaaa taaagagatc
     5761 tttccagcgt ttttgcgtcg ctacccatgg cagcacgctt aataccggca cggcaagggc
     5821 gagaaaacct ttggtcatca cccccatgcc gcaggtgatt cccagcagta aaaatcctgc
     5881 gcttttgcct ttccacgtct gtgcctgcat tgccagccag aagctgcaca ttcccgccac
     5941 cagccagaat gcaataaacg gatcgagcac ggcataagtg ccgatggcat agacaataaa
     6001 caatgagaga taaattactg tggcgagtag agccagacgt ttatcgcgcc ataagcgcag
     6061 agtaaaccag gtcaccagcg cggcagttaa cagggtcgca aagataacgc ctgcccgcac
     6121 accaaagtta ttcgcgccaa atagccattg cccaatgctg ttaatccagt atccggcaat
     6181 gggtttttcg aaataacgta gccctaacag atggggcaca atccagtcgc cggatgccag
     6241 catttctcga ctgatttccg cataacgcgt ttcatcgggt tgccagagca gacgcgtgct
     6301 gatcggtaac aggtaatagc aggcaataaa cgcgaagagg ccgataaggt aacgtaccga
     6361 tttcatcagc gactacccgc aatttgttgg caacccagcc agccttcacg tccggcaata
     6421 tttccgcgaa caacttgtcc gagcggcaac gtctctgata acagttcgct taaagggcaa
     6481 aatgtcactc cttcctgagc tgcgcgtttg aggagatcca caaaattatg ctgataagcg
     6541 cagccttcaa cttctgcatg aatggtataa accggcgtgc ctttatctcg caggatgcgg
     6601 ttgagtaacc aaccgttaaa atcttctgct ttcacatccc gaccaatcac ttcatcccag
     6661 gtgggtaagg tcaccggaat ttgcgcagtg ccagggtttc ctgattcgag caatggacgg
     6721 aacggcatgg ccccacgaca atcgctgttg tagcgcaaat ggaacgcttc ttttgcttcg
     6781 atcaccttct ggtcggcacg ccagcccgca gcggcagaac aggttaccgg ttgaccgata
     6841 atctcttcca gagtgcgaag accgcgtgca atatcgtcga tcattgtttg ccgatcccag
     6901 ttaccgctac gggcttgcca ggcatggtga tcccaggcgt gcaggccgac ttcgtgatgt
     6961 ttagccgctt cacgaatgat atcggcattg gcatgaccaa tctctttacc tggccaggcc
     7021 gtacctgcca gtaaaatatc ccagccataa agcgatgccg catttgagcg cagcatcttc
     7081 cacaaaaact gtggcttcac cagtcgccag agatggcggc ccatattgtc cgggccgacg
     7141 ctgaaaaaaa tgctggcctg aatattatgc ttactcaaga tttccagcag acgcggcacg
     7201 ccttcacggg tgccacgaaa ggtatcgaca tcaatgcgta agcctacttt ggtcatgatg
     7261 gtttatccgt aagatcaacg gtgcgcagga agaaatccag cgtttcgtcg atggtttcct
     7321 gcatatcaat tttcggctcc cagtccaggc agcggtgggc attgcggatg ctcggtttac
     7381 gatgctctac gtcctgatat cctttgccgt agtagctgct actttcgaca acgcgaaagc
     7441 ccgcaaacgg tgggaaatga tggcgcagcg gatgtttttc gaagctcgcc agcagcatct
     7501 cgcccagttc ctcaatgctc gcttcgttct caggattgcc aatgttgata atttcaccgt
     7561 cgcagcgatt tcccgcattt tcgataatgc ggtataacgc ctcgatacca tcgcgaatat
     7621 cagtaaagca gcgtttttgt tttccgccat caatcagctt aatcggtgaa ccttctacca
     7681 gattgagaat gagttgcgta atagcgcggg agctgccgat tcgtgccgca ttaaggttat
     7741 ccagtcgcgg tcccatccag ttaaacgggc ggaagagggt gaactgtaaa ccctcttttt
     7801 cgccataggc ccagatcacc cgatcaagta attgttttga taccgaataa atccagcgtg
     7861 gtttattcac cgggccgacg attaaattag aatggtcctc atcgaagtat ttatcgctac
     7921 acatcccata aacttctgaa gttgacggga agatgattcg cttacggtac ttcacgcagt
     7981 agcggataat gcgcagattc tcttcaaaat cgagttcaaa tacgcgcagc gggttgcggg
     8041 tatattcaat cggcgtggct atcgccacca gcggcaagac gacatcacat tttttgacgt
     8101 gatactcaat ccattcggaa tgaatactga tatcgccttc aacaaagtga aaatgcggat
     8161 gattcagaaa acggcttatc gcatcactgc caatatccag accgtaaact tcataatgat
     8221 cttcgcgcag caggcgttct gtcagatggt tgccaataaa gccattcacc ccgaggatga
     8281 gtacccgggt acggcgtcgg gcggtgcagg caggctggct attcaagcgt gaaccttgca
     8341 ccaggcccag cgtctgcgct aattgcgagc cctgcatagt aatgccgtcg cccgcctgtc
     8401 cggtgacgat ttccagcgcg ccatcgccac aggcaatcag cagtggcgca acagaaatca
     8461 cgctccccgg ctgtgctttg ctggcatgag gatgaacacg cgacgaccag acggtgaatt
     8521 tctgattgcc aacatagctg aaggcacccg gccacggatc ggcaacggca cgtaccatgt
     8581 tgtgcagtac ggatgccggt ttatgccatt caaggaaact gtcatccggc gttctgcgac
     8641 caaaacaggt ggcttcgttt tcgcgctggg cgatttccag aatattaccg tgtttaatgg
     8701 cgggtaatgt ctgttccagt agctggcgcg cggcatggca caatttatga tgcagcgtaa
     8761 tagcgatatc gtctggcgca atggcaatgc gcagttgcgc cacaatggcc ccggcatcgg
     8821 cacgtttcac cattcggtgc aatgtaacgc cagtttccgt ttcaccgttg accagcaccc
     8881 agttcagcgg cgcgcgacca cgatattttg gtaacagcga accatgcaga ttaaatgcac
     8941 ctgcgggagc gagctgcaaa atttcgtcgc aaataagatg gcgataataa aaagagaaaa
     9001 tcacatctgg cgacagttgg gcaatgcgtt ccacccacag cggatgatta acgtcatccg
     9061 gcgcataaac cggaatgcct ctttccgctg ccagacgagc caccgaacca taaaaggctt
     9121 tttcaccggg attatcggta tgggtaaaaa tggcgctaat ttcgtaaccg gcagccagca
     9181 gagcttcaat accgaggcat cccatatcgt ggtaggcaaa aacgacggtt ttcattattc
     9241 attttccttg ctggatggac ggataacttg ctgaacaaaa tagcgggggc gggcgcggac
     9301 atcggtgtag atcctgccga tatattcacc gagtaatccc atgccgataa actgagcgcc
     9361 aataaaagta aacagcacgg caaatagcat aaagacgcct tctgccgccc attgtggtcc
     9421 gaaggtcagg cgtaaaatca ccagcagcac cgcaatgcta aaacctccaa tcgcaataat
     9481 gctgccgagc agactcagca tacgtagcgg cgttgtggta aggcaggtca ccaggtcgta
     9541 catcaaatta atcaggcgca taaaactgta tttggattca ccaaactcac gctcggaatg
     9601 atgtactgga atttcaatgg cacggcgggc gaagatattc gccagaatcg ggataaaggt
     9661 gctacgttca tggcagtgca acatcgcatc gacaatatga cggcgatagg cgcgcagcat
     9721 acaaccgtag tcacccatcg ctttgccggt ggtgcgctga ataagccggt taatcatctt
     9781 cgaagctgtt ttacgaaacc agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc
     9841 gtaaccttca tcggcttttg ccaccaggcg ggggatttct tctggcggat tctggagatc
     9901 ggcatcaagg gtaataatta agtcgccggt aacgtgactg aatcccgcca taatcgctga
     9961 atgttgcccg taattgcggt taagcaaaat agacacaata tggctgttct ccgcttgtga
    10021 ggcttcgacc agcatatgcg cggaattatc gctactgccg tcatcaatca gcaggatctc
    10081 atactctttc cccaacgatt cacaggctgt ggtggtgcgc ctgattaatt ccggtaagct
    10141 ttcctgctcg ttataaacgg gaataaccac cgagactttc ttaacagggt ggatttcaaa
    10201 catggcttat tgtcctgcga gttgctgaag ggctgtgatg acacggtcgg catcggcggt
    10261 agtcatatcc gggaacagcg gcaaagaaca gatgcgttcg ctattccatt cggtattcgg
    10321 taacgacagc gtgggaaaac gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg
    10381 taacccggta ccaatgcctc tttcttttaa cgcttccatc aacgcatcgc gactgatacc
    10441 acaacgttgt tcatcgacac gaataataaa cagatgccag gcgtgaacgt gcggccaggc
    10501 gggaaggctt aatggctgaa aggggagagc tgccagtgct tgctgatatt gctgggcaat
    10561 ttcgcgccga cgggtgttga ggtgctctaa ttttgctaac tgtgtcaggg caattgcggc
    10621 gttaatatcg gtcagattgt acttatagcc cggtgttaag acttcagcct gcggtgcacg
    10681 gccccaggtt tgtctgtcat aggcatcgac acccagaccg tgaaatttca gcatccgtag
    10741 ctggcgggca aggttttcat tatcagttac aatcaggcca ccttccgcac aggtaatatt
    10801 tttaatggca tgaaatgaaa aaatagtggt accttttgcg ccaatatgtc gccctttgta
    10861 atacgtaccg acggcatggg cagcatcttc gataactgcg atgccgtaac gttcgccaat
    10921 ggcgcgaatg gcgtcaatat ctgctggcgc accggcataa tgcaccggaa tgatggcttt
    10981 agtgcgtggc gttatggctg actcgatcgc ttcaggcgtg accatcggcg tatcgcggtc
    11041 gacatccacc attaccggcg ttgcacccaa caaggaaatc atattgaggg ttgaaaccca
    11101 ggtcagggaa ggcgtaatca cttcatcgcc cttgccaatt tccaacgcca ttagcgtgat
    11161 atgcattccg gcggtggctg aactgaccgc gatggcatgc tgatttcccg tcaactggca
    11221 aaaggcttgc tcaagcgcct gattcttcgg accggttgtg atccaaccgg attcgagaac
    11281 ctctttaact gcagcgagtt cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc
    11341 tgacattgct tttccttccg ccattgaata cctgtccact tatatttgct ataaagagtg
    11401 ttgtgtatat tttgccattt ggagcgaaat tttaaggata gaatattaac ttaaccttaa
    11461 gaaactaata ttagacgtaa atattgaaat ttttatattt tttcttattt aggctttgca
    11521 tttggcaaaa ttttgaggca ttttgccgac atcgtaggat ttttaatatt acaccaactg
    11581 ctaattatcg ccagaaatgt cgctcaattt tacggtaatt gtctggttgc gcttgtctat
    11641 aggtggagtt tacgtgttag ctttttgccg ctcatcattg aagtcaaaaa aatatttcat
    11701 cattttactg gcgctcgctg caattgccgg actgggtact catgccgcct ggagtagcaa
    11761 tggtttgcca cgtatcgaca ataaaacatt ggccagactg gcacagcagc acccggttgt
    11821 cgttttgttt cgccatgctg aacgttgcga tcgttcaacc aatcaatgct tgtcagataa
    11881 aacaggtatt acggttaaag gtacccagga tgcccgtgaa ctgggcaacg cttttagcgc
    11941 tgacatccct gatttcgatc tttattccag taataccgtc cggaccattc agtcggcgac
    12001 ctggttttcc gcgggtaaaa agctgacggt agataaacga cttcttcagt gcggtaatga
    12061 gatttatagt gcaattaagg acttacaaag caaagcgcct gataaaaata tcgttatttt
    12121 cacccataat cattgcctga catatattgc taaaaataag cgtgatgcga catttaaacc
    12181 tgattatctg gatggtttag tcatgcatgt ggaaaaaggc aaagtttatc tggatgggga
    12241 attcgttaac cactaaattt tcaatctgac agccagtgat ggctgtcatc attgttaaag
    12301 aagacctttc aaacgtaacg tttttcgggt ggcaacattt aaatcataat gcaccagatc
    12361 ttcaggtttt acccacgcgt agtcctgaaa ctcttcgttt attttcactt ctcggttggc
    12421 agaaacgcag tcaaaaatca ggtaaatcat ataaatctct tccttgcgac catctgcata
    12481 cgtcttggtg cgaatatcat cgctgaaggt ccacggcgtg atttctgtca aaagcagctg
    12541 ttctcccagt tcttcgcgaa tttcgcggcg tagtgcctct tcaattcgtt cgccagactc
    12601 cacgccgcca cccgaaagcg cccattgacc ggggaaaacg ccgcgatcgt cggccatttt
    12661 acacagcaaa taagcaccat cattttgaat caaagggcat acaatagtcc gttgtcgcat
    12721 aggggtattc cttataaata aaaagaaacc atgagaatac ggtaactcag aaatatgtga
    12781 aagtgacagt aatctctttt gcaatgataa ctgcacttat tattattttt gtgtgtatcg
    12841 gtaaagtgtg tttatttttt cggcgtttaa ttatttaaga agtatttagt tacaataagt
    12901 gactagatcc cttttttcgc tcattgaatc ctctgtgcca ggcagtacaa tcccgcagct
    12961 ttgggaattt tgattaattg cttgtgggaa aggggataat gaaaaaaatt gcgctagcag
    13021 gtctggccgg aatgctgttg gtttctgcat cggtcaatgc aatgagcatc agcggccagg
    13081 cgggtaaaga atacaccaat attggtgtcg gttttgggac tgaatcgacg ggcctggctt
    13141 taagcggtaa ctggacacat aacgacgacg acggtgacgt cgcgggcgtg gggctggggt
    13201 tgaatctgcc tctcgggccg ttaatggcga ccgttggcgg aaaaggcgtt tacaccaacc
    13261 cgaattacgg cgatgaaggt tatgccgcag cggtaggagg tggtttgcag tggaaaattg
    13321 gcaacagctt ccgtttgttt ggcgagtatt actactctcc ggattcgctc tccagcggta
    13381 ttcaaagtta tgaggaagcg aatgctggcg cgcgttacac cattatgcgt ccagtcagta
    13441 ttgaggcggg ttatcgctac ctgaatctgt cgggtaaaga cggtaaccgc gacaacgccg
    13501 tggctgacgg cccgtatgtt ggggttaacg ccagtttctg atcccttccg gcgcggcatt
    13561 ctgtcgcgcc attcagtttc ttactacgcc ttatctccct ttgcgttata gtgattcgat
    13621 aacaaaagca ggagataacc atgttaaaag tggaaatgct atccaccggg gatgaagtgt
    13681 tacacgggca aatcgttgac actaacgctg cctggctggc cgattttttc tttcatcagg
    13741 ggctgccatt atctcgccgc aatacggtgg gggataacct tgatgactta gtcaccattc
    13801 ttcgcgaacg tagtcagcac gccgatgtgc tgatcgttaa cggcgggctg ggaccgacca
    13861 gcgatgattt aagcgcactc gccgctgcga cagcaaaagg tgaaggcatg gtgctgcatg
    13921 aagcctggct caaagagatg gaacgctatt tccacgaacg tggacgagta atggcaccga
    13981 gcaaccgtaa acaagcggag ctgcctgcca gtgctgaatt tatcaataac ccggtaggca
    14041 ccgcctgtgg ttttgccgtg cagcttaatc gttgcctgat gttctttact cccggcgtac
    14101 cgtcagaatt taaggtgatg gtcgagcacg aaatcctgcc gcgtctgcgc gagcgttttt
    14161 ctttaccaca gccgccggtt tgtctgcgtt taactacctt tggtcgttcg gaaagcgatc
    14221 tggcacaaag cctggacact ctacaactgc cgccgggcgt aacaatgggc tatcgctctt
    14281 caatgccgat tatcgaactg aaactcaccg gaccggcaag cgagcaacag gcgatggaaa
    14341 aactgtggct ggacgttaaa cgtgttgccg gacagagcgt gattttcgaa ggcaccgaag
    14401 gactgcccgc gcagatcagt cgcgaattgc aaaaccgcca gttcagcctg acgttgagcg
    14461 agcaattcac cggtggttta ttggctttgc aactttctcg cgcaggtgct ccattgctgg
    14521 cgtgtgaagt ggttccttca caggaagaaa ccctggcgca aactgcgcac tggattacag
    14581 aacggcgggc caaccatttt gccgggctgg cactggctgt ttcgggtttc gagaacgaac
    14641 atctcaactt tgcgctagcc acaccagacg gcactttcgc tctgcgtgtg cgtttcagca
    14701 ctacgcgcta cagcctggct atccgtcagg aagtgtgcgc aatgatggca ctgaatatgc
    14761 tgcgccgttg gttaaacggc caggatatcg ccagtgagca tggctggatt gaggttattg
    14821 agtccatgac cttatctgtc tgattccgcc actctgtgcc ggtttactgc accggcacat
    14881 tccccctccg gcaaaacttg tccaaccctc cctacggtga gcgtggtgtt ggggccagtg
    14941 tggcacgggc tgcgcgctgg ggacgcattg agaattacat ggcgcaagtc aacgattcgc
    15001 tttgtctgtt agtgcaggtg gaaagtaaaa cggcactgga taacctggac gagatcctcg
    15061 acgtcgaagg gattgatggc gtgtttattg gaccagcgga tctttctgcg tcgttgggct
    15121 acccggataa cgccgggcac ccggaagtgc agcgaattat tgaaaccagc attcggcgga
    15181 tccgtgctgc gggtaaagcg gctggttttc tggctgtggc tcctgatatg gcgcagcaat
    15241 gcctggcgtg gggagcgaac tttgtcgctg ttggcgttga cacgatgctc tacagcgatg
    15301 ccctggatca acgactggcg atgtttaaat caggcaaaaa tgggccacgc ataaaaggta
    15361 gttattgata tcaaaggccc atggggatcg gctgtgggcc tgtgttaatt agtggttatt
    15421 cgctgccaga tcggcttcgc ttagctgggt ggccgcgagc acctggtcac gatcaatgcc
    15481 ctgttccagc atgcgcaaag caattttaat ggcttgttca tgcatacctt cctgccagcc
    15541 aatttgatgc ccttcctgcc gtagccgttc agcaatagtc attaatctct ccttttgtag
    15601 tggtgaacgt tcggcaatct cctcaataaa acgggtgaaa cgggaggtat cgccgcattg
    15661 cagcagataa ttaaacagtg tttgtagctg gctgtcatta gtgaagcctc taaccaaaag
    15721 cgtggtgatc ctgtcgacca tgccgattaa atcgcggtcg cgaatatgct tttgaatcag
    15781 ttccagcaga gctatacgcc gatgttgcat gatctcatcg tcaggtacga tggtgatatc
    15841 caccaacgga aaagcttcgg tgtacaactg ccgggcaagt tgcggatcgt caaactcatc
    15901 cagccagtta agtgagtacg ggtagggcga tgtttcgcca tgataaaaca gcaatggcac
    15961 caccagcgga actctgtcat agcctttatc caggtgacgc tgcatggcgg cagtggcata
    16021 gcgcattagc cgaaaagcca tattcttttc tgcagagctt tgatgttcaa tcacgcagta
    16081 gatatagccg tcaccgtcgc tggtttccac ggaccacaaa acatccgagt agtaagcgcg
    16141 taaacttttt tcaataaaac tagtgggttc caggcgtaag gtttgcaggt tgcaaagctt
    16201 gcgcagtggt tctggtaaat gtatttcgag aaaatcccgt gcggtttcgg gtgtgaacat
    16261 aaaggtttta aacaccgcat catgcgggga ggatgttgtt gattcggtca tggttttccg
    16321 tcacctgata tttatgatga cgcgacatta cgtctatccc gggcctttga catctgtcag
    16381 tttcagcctt tgcgagacat tttcagagga attttcaaag cgttgcaacg catacatggt
    16441 gcgtggaatc gcgctcgcaa aaatgcccga taagcaatgt gcttaccggg catcaaaagg
    16501 tcggttaagc cagcgcctgg gccagtagcg taatcggatg ttcgcagcga aggctcgtgg
    16561 acatctcaat ctgccatttg caggtttcgc agtcggtgat gaccagatcc gcgccgctct
    16621 cttctatctg gcggaacagt ggcgcaccga tggcttgtga ggtggggtag ttctcttttt
    16681 tgaaaccgta agtacccgca ataccgcagc actgggaatc cagcaccgtt aactcaagcc
    16741 ccgggatttt acgcaacagc tccagggtgt agagcgtcca gcccattttt tccatatggc
    16801 acggagtgtg ataaaccact ttcagcggca gcggtttcag cggtaacgtt ttgccttcgt
    16861 ccagcttgcg ccacagccag cgggttgcca gttcgatatg atcgcgcaag cctttgttgt
    16921 cgacattcag cacttccggg tattcgtcgc gcagggcaaa tgtacaggtt gaggaggtgg
    16981 caatcaccgg aatgcctttt actcccacag cttcgcggat cgactctaca ttcgtaattg
    17041 cctgtttgcg tgctttatcg gtaaagccgt tggcgattag cggtacgccg cagcattttt
    17101 ctttgctgag cagttgtaca ccggtaccca ttgcgttgag cactttaatt aaatctttac
    17161 ctaactgcgg atggttgtag ttaacgaagc agccgtgaaa gaaagcgacc tggtctttat
    17221 attgtgcttg ctgagccgct atgctgcgat accagcgacg aaacgtaccg aaggagtatt
    17281 tcggtagcgt gcggcgatga tcgattttta acgccgcatc aagcagctgc cgcaccggtt
    17341 tcagcgaggt agcggtgttg acgattggtg cgaacggcgt ggaaacgcta cccatcaggt
    17401 cggtatgact caacacaaaa ttacgcagcg acgggcgcgt ggtgtcatat tttgcccgcg
    17461 cgcgctggat aatatcgcca atcttcacat cggacgggca ggcgacttca caacgtttgc
    17521 agttgatgca atatttcagc gcctcgtcat acagtgcgcc atctttcaaa cgcagacgct
    17581 cgccatccgg cccggcttgt tttggccctg gataaccggg attcacccgg ctcaccgggc
    17641 aggcggtggt gcagacggtg cacttaatgc agttttcgaa gctggtgtcg ttcattgttg
    17701 gcctcctgcg cgttgggcaa tctgttgagc ggcatgtaaa gcactgacgg cacaaacacc
    17761 gccgccgcat ccctgggcga tgggatcaaa tccgcccagc accgaaccga tggcaaacag
    17821 gttctcaatg gtttgccctg cctgcgacgg gcgtagcgtc tgatcagtgg ttacaccgaa
    17881 ctgctgccac ggttgcggcg caaaaaaatc gcccttatac cattcacccc gcgtggcggt
    17941 ttgtagcaca tcaaggccga gaatcggctc gcgaatgccg ttacgttccg ctaccagtcc
    18001 gccactaaag aaactgccgc tggcgagaac cgcgaaacgt ggacgtagcg gaatatcggc
    18061 gtgattgcgg gtccagattt cattcactac gccattttta caggtcactt ttttcacttc
    18121 atcgcccggc atccacacgc caccctggcg cacaaactgg cgctgtaact ggttttgcag
    18181 acgaatgccc agcacggaag gcggcagcgt tggcaaaagc atcagtgaac aaggtagttt
    18241 ttcattcaac caacgccaca gtttgtcatc ggccaggccg aagcaggcgg gcatcaggat
    18301 catttcgcag gtattggcga caggaataag cgcatcaagt aacagcggcc agttttcttc
    18361 attatcaagg aaacgggcga tattcaccgc gcgaaattcg gtggcgttat tgcgcaggac
    18421 atccagttcc ggcagctcta tttctgcggt ttcaaccgaa agatccagtt cgcgcaacga
    18481 agccgccgca agatgcgcct ggaaatccat cagcccgctt atccccacta cacatatctt
    18541 cttcgcgggt agcggccaga cgggaacttc tggcgaactt aaccaggttg cacgcaaagt
    18601 gccgagcggg gtaacgcgct gatgggccag ttccacgctg ccctgcaatt gcgcaccgct
    18661 ttcagcgatc aatgcctgcg cctggcaagc gagatcgagc acgcgttgtg gttcgagaag
    18721 actgtaagga tgggctggtg cctgctgacg caaagattcc agtccactgt gaatgtctgt
    18781 caccggttga ccatcaggca gatggctcag caaatccagc gaaccggatg agaaatgcag
    18841 cgcgctttga ccacgcgtga caatggcaca gcgcaggccg tgtttttgca gttgcaggcc
    18901 acagagtaat ccggcgaggc cgccgcccat aatgacagta tcaaagcgca tctttctgct
    18961 ccttctccag accacacaat ccctgataaa cccagcgggt aaattcgctt tcgcgcagtg
    19021 catctcccca ggcgatgggt tgcacgcctt tccagcgttc gttaaggaag gtggaaagtt
    19081 gctcgataga ttgcgcggac gtcgtgacgt taaaacgttg cagcagtccg gcagcgcggc
    19141 aggcgcagag ttcgccctgg caggtaccca tccccacacg ggtacgacgg cgtaaatcca
    19201 gcaggctatt aacgtttaaa ttttctaccg cgtactgaac ttcacccgca gttaccgctt
    19261 cgcactcaca taccaggcta cgatgcagac ggccttcgct cagccaggca ggcgtgcgat
    19321 cgccatgacg ataaaccgca gaaccgcgca gcggggcagg cagggagatg actttacgca
    19381 aggtaacttc agcgggttct tgtgaaccag gcagtgccag atcggcagtc gtacaggggc
    19441 gcgtgttgcc cagtttgcgg cataccgcgt cggtagccca ttcagccatc agccgatagg
    19501 tcatcagttt gccaccggtg atggtgataa atccgtccag accatcgcgt tcagcatggt
    19561 cgagcagcac gatgccacgg ctgacgttac gtccgctcgg gtcgtcatcg ctggcaacca
    19621 gcgggcgcac gccagaatag gcccgcaaaa tgcgcgtttt cgccatcacg ggggccagtt
    19681 tttccccttc acgcagcaga atatcaacct cttctgccgt cactcgatta tcgtcaatct
    19741 cgttgtaatc aatacgtaaa gaggtggtgc caatcagcga aatggtatcg ccaggcacca
    19801 gaatatcggc gtcggaaggt ttacggcagc ggttgatcac atgctggtta atgcggtgat
    19861 ccatgatcag cagcgatcct ttcgccggga acatgcgaat gcgcagatcg gcatattcgg
    19921 caatgtgttg cccccagatc ccagcggcat taaccacgac aggtgcatga agggcctgag
    19981 tttcgccggt gagatggtta cgtacacgaa caccgcacac cgtcgcgcct tcacgaatca
    20041 gccccgtgac ttcatgagcg gtaaggataa cggcaccgtg ttctttggca tccagcatgt
    20101 ttgctgcggt cagacgaaat ggatcaacgg tgccatccgg aactttcacc gcgccaatca
    20161 gtgccgggtt aacggcaggt tcgataatgc gcgcttgctg cgggtctata gcttctgcgc
    20221 tgatccctgc ttcttcgcag gcgcgaataa aagtggcctg gaatgagagg tcatcttccg
    20281 gcagggtgat aaacaggccg ttggttggtt caacgcagtg acgtgcaatg cgtttcagga
    20341 tctggttttc actaatgcat tcgcgggccg attccgcatc ggttaccgca tagcgcgcac
    20401 cgctgtgcag caggccgtgg ttacgcccgg tggcaccggt tgcgatgtcg tggcgctcaa
    20461 ccaaaatcac gcgcagcccg cgcagggcac agtcgcgggc aatcccggct cccgttgcgc
    20521 cgccaccaat gataatcacg tcacttgatt gcgagtcgcg agttttcatt gttatccctc
    20581 tgaagttcgt tttttaccat ttagccatag taaaaacatg aattgtttga tttcgcgcat
    20641 atccgctcat aattcgaaag tgaaacgtga tttcatgcgt cattctgaac attttgtaaa
    20701 tcttatttaa taatgtgtgc ggcaattcac atttaatttc tgaatgtttt cttaacatcg
    20761 cggcaactaa agaaacggca ggttctccca ctgaatcagt ctgttaatca taaataagac
    20821 cacgggccac ggaggctttc aatgttgagt atttttaaac cagcgccaca caaagcgcgc
    20881 ttacctgctg cggagatcga tccgacttat cgtcgattgc gctggcaaat tttcctgggg
    20941 atattctttg gctatgcggc ttactatttg gttcgtaaga actttgcgct tgctatgcct
    21001 tatctggtcg agcagggatt ctctcgcggt gatttaggtt tcgcgctttc ggggatctcg
    21061 atcgcttatg gattttcgaa attcataatg ggttcggtat cggatcgctc gaatccgcgc
    21121 gttttcctgc ccgcaggttt gattctggcg gcggcagtga tgttgtttat gggctttgtg
    21181 ccatgggcga cgtcgagcat tgcggtgatg tttgtactgt tgttcctctg cggttggttc
    21241 caggggatgg ggtggccgcc gtgtggtcgt actatggtgc actggtggtc gcagaaagaa
    21301 cgtggcggca ttgtgtcagt gtggaactgt gcgcacaacg tcggtggtgg tattccgccg
    21361 ctgctgttcc tgctggggat ggcctggttc aatgactggc atgcggcgct ctatatgcct
    21421 gctttctgcg ccattctggt ggcattattc gcctttgcga tgatgcgcga taccccgcaa
    21481 tcctgtggct tgccgccgat cgaagagtac aaaaatgatt atccggacga ctataacgaa
    21541 aaagcggaac aggagctgac cgcgaagcaa atcttcatgc agtacgtgct gccgaacaaa
    21601 ctgctgtggt atatcgccat cgccaacgtg ttcgtttatc tgctgcgtta cggcatcctc
    21661 gactggtcac cgacttatct gaaagaggtt aagcatttcg cgctggataa atcctcctgg
    21721 gcctacttcc tttatgaata tgcaggtatt ccgggcactc tgctgtgcgg ctggatgtcg
    21781 gataaagtct tccgtggcaa ccgtggggca accggcgttt tctttatgac actggtgacc
    21841 atcgcgacta tcgtttactg gatgaacccg gcaggtaacc caaccgtcga tatgatttgt
    21901 atgattgtta tcggcttcct gatctacggt cctgtgatgc tgatcggtct gcatgcgctg
    21961 gaactggcac cgaaaaaagc ggcaggtacg gcagcgggct ttaccgggct gtttggttac
    22021 ctgggcggtt cggtggcggc gagcgcgatt gttggctaca ccgtggactt cttcggctgg
    22081 gatggcggct ttatggtaat gattggcggc agcattctgg cggttatctt gttgattgtt
    22141 gtgatgattg gcgaaaaacg tcgccatgaa caattactgc aaaaacgcaa cggaggctaa
    22201 tggcatgaaa ttgacgctga aaaaccttag catggcgatc atgatgagca ctatagtcat
    22261 gggaagcagt gcaatggcgg cggacagcaa cgaaaaaata gtcatcgccc atcgcggtgc
    22321 cagtggatat ttgccggagc atacgctgcc agcaaaagcg atggcgtatg cgcagggagc
    22381 ggattatctg gaacaggatt tggtgatgac caaagacgac catctggttg ttctgcatga
    22441 ccattatctc gatcgtgtta ctgatgttgc cgatcgtttc ccggaccggg cgcgcaaaga
    22501 cggtcgttac tacgcgatag atttcacgct ggatgaaatt aagtcgctga aatttaccga
    22561 aggtttcgat attgaaaacg gtaaaaaagt gcagacttat ccggggcgtt tcccaatggg
    22621 taagtccgac ttccgggtgc acacctttga agaagagatt gaatttgttc aggggttaaa
    22681 tcactctacc gggaaaaata tcggtattta tcctgaaatc aaagcgccgt ggttccatca
    22741 tcaggaaggg aaggatattg cggcaaaaac gctggaagtg ctgaagaaat atggttacac
    22801 cggtaaggac gacaaagttt atttgcaatg ttttgatgct gatgagctga agcgtattaa
    22861 gaatgagctg gaacctaaaa tgggcatgga tctcaatctg gtacagttga ttgcctatac
    22921 cgactggaat gaaacgcagc agaaacagcc ggatggaagc tgggttaatt acaactacga
    22981 ctggatgttt aagccgggtg ctatgaaaca ggtggcggaa tatgccgacg gaatcgggcc
    23041 ggattaccat atgttgattg aggaaacatc gcagccgggt aatatcaaac tcactggcat
    23101 ggtgcaagat gctcagcaga ataaactggt agtgcatcct tataccgtgc ggtcagataa
    23161 actgcctgaa tacactactg atgtgaatca gttatatgat gctctgtata acaaagcggg
    23221 tgtaaatggg ctgtttactg atttccctga taaggcagta aaattcctta ataaagagta
    23281 atgctactgc aggatggcgt tcatgcgcca tcctgtctca catcaaattt ttccattcac
    23341 cacgagttgt tccgtacacg tttgtagtaa aaacagtgga ctgccttcta ccggcttttc
    23401 tttagccaac aacgaaatct gtcgatagaa ggttggttcg tgaggaacgg aacacaattc
    23461 acctgcaatg cttttcagcg ttaactctgg ttgtaaggcg atgcctaatc cctgtcgaat
    23521 aaacttcatc gctgtatccg actgattaaa ttcacatttt atctactggg cgcactatta
    23581 gtggtagtgg ctggtggaat ctatgcgctg caaatgaagg gtaaaagcgg tgtcacgcta
    23641 gtagtggcaa aagaaattca ttaatacgac acgtttcatt aagattttcc tcaggtaacc
    23701 acctatagtc attgtcgtta ataaaaatga caggcgatag ggtatggcag tttcagcaaa
    23761 gtacgacgaa tttaatcact ggtgggcaac agagggcgac tgggttgaag agcctaacta
    23821 tcgccgtaat ggtatgagcg gggtgcaatg cgtcgagcgc aacggcaaaa agctgtatgt
    23881 aaagcgcatg acgcatcatc tgtttcattc cgtacgttat ccgttcggcc gaccaacgat
    23941 tgtccgtgag gttgcggtaa ttaaagaact ggaacgggcc ggtgtcatcg tgccgaagat
    24001 cgtttttggt gaagcggtga aaattgaggg tgaatggcga gcgttgctgg tgactgaaga
    24061 tatggcgggg ttcatcagca ttgctgactg gtatgcccag catgcagtat cgccttattc
    24121 tgacgaagta cggcaagcca tgttgaaagc agtggcgctg gcgtttaaga aaatgcatag
    24181 cattaatcgt cagcatggct gttgttatgt tcgccatatc tatgtgaaaa cagaaggtaa
    24241 tgcagaagct ggttttcttg acctggagaa aagtcgtcgc cgcttgcgtc gcgataaagc
    24301 gattaatcat gacttccgcc agctggaaaa atatctggag ccaatcccca aagcggactg
    24361 ggagcaggtg aaagcgtatt actacgccat gtaatcgttg taggccagat aagacgcata
    24421 agcgttgtat ctggcactac aaccaattca catctcgatt tcaatatcgc cttttgcccg
    24481 gcaacaacag ggcaaaattt cccccggctg aataaaggct aacggttcgg caatccagtc
    24541 aacctgacct gcaaccagac gggtgcgaca ggagccgcag taaccttcgc gacactgata
    24601 ctcaaccgcc acattgtggg attccagcgc cgccagaagg gaagggtgtt catcctggca
    24661 cagcagttgt gtgccagtga tgcgcagggt aacgcgggcc atcagagttg gaagttactc
    24721 aaatcgtcgg tgtccacttc cgcgtcaatc tgcccgacca gataagaact gacttccact
    24781 tcctgcggag caacctgcac gttatcagac accagccagg tgttgatcca tgggatcggg
    24841 ttggagcgcg tctggaacgg cagatccaaa ccgactgcct gcatacggat attggtgatg
    24901 tattcaacgt actggcagag aatgtcttta ttcagaccaa tcatcgaacc gtcgcggaac
    24961 agataatccg cccagtcttt ctcctgttga gctgcctgaa caaacaggtc atagcactcc
    25021 tgcttacact cttcggcaat ttccgccatc tcaggatcgt ccgcgccgct gcgcagcaga
    25081 ttcagcatat gctgggtgcc ggtcaggtgc agggcttcgt cgcgggcaat caggcgaata
    25141 attttggcgt tgccttccat caattcgcgt tctgcaaatg cgaaggaaca agcaaagctg
    25201 acgtagaaac gaatcgcttc cagcgcgtta acgctcatca ggcagagata cagttttttc
    25261 ttcagctcgc gcaggctaac ggtcacagtt ttaccgttaa cggtgtgggt accttcgccc
    25321 agcagatgcc agtagctggt catttcgatc agctcatcgt aatagctgga gatcccttcc
    25381 gcacgtttct ggatctgctc gttggtgacg atatcgtcaa acacaacaga cggatcgtta
    25441 acgatattac gaatgatatg agtataggaa cgggaatgaa tcgtttctga gaacgcccag
    25501 gtttcgaccc aggtctccag ttccgggatc gagatcagcg gcagcagtgc cacgttcggg
    25561 ctacggccct gaatggaatc cagcagcgtc tgatatttca ggttgctgat aaagatgtgt
    25621 ttttcgtgtt caggcagtgc ctggtaatcg atacggtcgc gagaaacgtc aacttcttcc
    25681 ggacgccaga agaaagagag ctgcttttcg atcagctttt cgaagatgtc atatttttgc
    25741 tgatcgtagc gagccacgtt gaccggctga ccaaagaaca tcggttcttt gagctgatca
    25801 tttttcgtct gtgaaaaggt ggtatatgcc atgagtgtgt cctgtggttt tattacccct
    25861 caccctaacc ctctcccgca aggggagagg ggacggtttg gtgcaaaggc tgggggatta
    25921 tcagatctta catgcgccgc tttcgcagcc atcgtcctgg attgacggta ccagatcgtc
    25981 ctgcgcgtct tcagcgccgt cacgggtgtt ctgataatac agcgttttga ccccgaattt
    26041 gtaggcggtg agcaggtctt tcagcaactg ctgcatcggc acttttcctg acgggaaacg
    26101 tgacggatcg tagttggtgt tagcagagat cgactgatcg ataaatttct gcatgatacc
    26161 taccagttgc aggtagccat cgttacccgg catttcccac agcagctcat aggcgtcgtg
    26221 caggtgctcg tagtccggca ccacctggcg caaaataccg tctttcgacg ctttgatgct
    26281 gacgtaaccg cgcggcggtt caataccgtt agtggcgtta gagatctgtg aagaagtctc
    26341 tgacggcatc agggcagaaa gcgtggagtt acgcaggccg tgcgttttga ttgattcacg
    26401 caatgcttcc cagtcgtaat gcagcggctc attagcgatg gtatccagat ctttcttata
    26461 ggtatcgatc ggcaggatcc ctttcgcgta agtggtttcg ttaaaccacg ggcacgcgcc
    26521 ttgctctttt gccagctcgt tagacgcttt cagcaggtaa tactgaatcg cttcgaaggt
    26581 tttatgcgtc aggttgttgg cgctgccgtc ggagtaacgc acaccgtgct tcgccaggta
    26641 gtaagcgaag ttgatcacac caatacccag cgtacgacga cccatcgctc cacgtttggc
    26701 ggccgggatc gggtaatcct gataatccag cagcgcgtca agtgcacgaa ccgccagaat
    26761 tgccagctct tccagttcat ccaggctatt aattgcgccc aggttgaaag cagacagcgt
    26821 acacagcgcg atttcaccgt tctcgtcgtt gacgtcgttc agcggtttgg tcggcagggc
    26881 tatctccagg cacaggttag actgacgcac tggcgcgatg gccggatcaa acgggctatg
    26941 ggtattgcag tggtcaacgt tctgaatata gatacgaccg gtagacgcac gttcctgcat
    27001 catcagcgag aacagctcaa cggctttcac acgctgcttg cggatgctgt cgtctttctc
    27061 atatttggta tacaggcgtt caaactcttc ctgatcggcg aagaatgcgt cgtacagccc
    27121 cggtacgtcg gacgggctga acagggtgat atcttcacct ttcagcaggc gggtatacat
    27181 cagtttgttg atctgtaccc cgtagtccat atggcgcacg cggttgcctt ccacaccacg
    27241 gttgtttttc aacaccagca ggctttccac ttccagatgc cacatcgggt agaacagcgt
    27301 tgccgcaccg ccgcgcacgc cgccctgaga gcaggatttc accgctgtct ggaaatgttt
    27361 gtagaacgga atacagccgg tatggaacgc ttcaccaccg cgaatcgggc tacccagcgc
    27421 acgaatacgc ccggcgttga tgccgatccc ggcacgctgg gaaacgtatt tcacaatcgc
    27481 gctggaggta gcgttgatgg aatccagact gtcaccgcac tcgatcagta cgcaggagct
    27541 gaactgacga gtcggggtac gcacgccgga catgattggc gtcggcagcg aaattttaaa
    27601 tgtggaaacc gcgtcgtaaa aacgcttcac atattgcagg cgcgtttcac gcgggtagtt
    27661 cgagaacagg cacgcggcaa ccagaatata aaggaactgg gcgctttcgt agatttcgcc
    27721 ggtcacgcgg ttctgtacca gatatttgcc ttccagctgc ttaacggcag cataagagaa
    27781 ggtcatatca cggtcgtgat cgataaacgt gtccatctgc ttgaactctt cttccgtgta
    27841 gtcttccagc agatgattat cgtatttgcc catctcgacc attttcacca cgtggtcgta
    27901 cagcgcaggc ggctcaaact ggccgtaggc ttttttacgc aggtggaaga tcgccaggcg
    27961 cgcggcgaga tactgataat ccggcgcatc acgggagatc aggtctgcgg ccgccttgat
    28021 aatggtttcg tggatgtcag aggtcttgat accgtcataa aactgaatgt gggagcgcag
    28081 ctcgacctgg gaaatcgaaa cgttatgcag tccttctgcc gcccaatcca gaacgcgatg
    28141 gattttgtcg agattgatgc gctctgtgct accgtcgcgc tttgtcacca gcagattctg
    28201 attcatgtat gtcgtacctg tttttggaat tctttccgca atagtttcat gcagactacc
    28261 tgtagtacgg gaagctgctt agaacactat atataggggg tatgtttggg attcactgca
    28321 agatagtgtg aaaatggccc tcttgcaagt gcataacttt gtggataact caggaaggaa
    28381 aaagtggctt tcgcgcacct taggtcagac aaggtgtccg ggaaagtcaa tgggaagaga
    28441 aaaatttgtt aaaaataact gttcggcgat ttcttgaacg gcagaatagg ttggataaat
    28501 tgatatagat ggtttattaa tctttttgct aatgattgca aaaattgatc taagcacgag
    28561 tcatcacata atctgatgta tcagttgtgg tcaatatata agcgaatgta agcgattgtt
    28621 gatgtcgaaa tggttgtttt cta
//
LOCUS       DAZKGS010000052        28544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKGS010000052 DAZKGS010000000
VERSION     DAZKGS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHOQY_002157"
     CDS             complement(<1..596)
                     /locus_tag="ACHOQY_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9678248.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHOQY_002158"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHOQY_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9678249.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHOQY_002159"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHOQY_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9678250.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHOQY_002160"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHOQY_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9678251.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHOQY_002161"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHOQY_002161"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9678252.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHOQY_002162"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHOQY_002162"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9678253.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHOQY_002163"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHOQY_002163"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9678254.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHOQY_002164"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHOQY_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9678255.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHOQY_002165"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHOQY_002165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9678256.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHOQY_002166"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHOQY_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9678257.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHOQY_002167"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHOQY_002167"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9678258.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHOQY_002168"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHOQY_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9678259.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHOQY_002169"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHOQY_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP9678260.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHOQY_002170"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHOQY_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9678261.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHOQY_002171"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHOQY_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9678262.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHOQY_002172"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHOQY_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9678263.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHOQY_002173"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHOQY_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9678264.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHOQY_002174"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHOQY_002174"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9678265.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHOQY_002175"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHOQY_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9678266.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHOQY_002176"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHOQY_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9678267.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHOQY_002177"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHOQY_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9678268.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQAEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHOQY_002178"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHOQY_002178"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9678269.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHOQY_002179"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHOQY_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9678270.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHOQY_002180"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHOQY_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9678271.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHOQY_002181"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHOQY_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9678272.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHOQY_002182"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHOQY_002182"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9678273.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHOQY_002183"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHOQY_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9678274.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHOQY_002184"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHOQY_002184"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9678275.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHOQY_002185"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHOQY_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9678276.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            26270..27076
                     /gene="nirC"
                     /locus_tag="ACHOQY_002186"
     CDS             26270..27076
                     /gene="nirC"
                     /locus_tag="ACHOQY_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9678277.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27095..28468
                     /gene="cysG"
                     /locus_tag="ACHOQY_002187"
     CDS             27095..28468
                     /gene="cysG"
                     /locus_tag="ACHOQY_002187"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9678278.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6877 a         7688 c         7233 g         6746 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat caggcggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaactttt tgttttattt tttgttttta ttttttaaag gataatcaaa tgtttacaga
    26281 cactattaat aagtgtgcgg ctaatgctgc acgcattgca cgcctgtcgg caaataaccc
    26341 gctcggcttt tgggtcagct ccgccatggc gggcgcatat gtgggtcttg ggatcatcct
    26401 gattttcacg ctcagtaatt tgctcgaccc gtccgtacgt ccgctggtga tgggcgcgac
    26461 ctttggtatc gccttaacgc tggtgattat cgccggttct gaactgttca ccggacacac
    26521 catgttcctc acctttgggg taaaagcggg cagcatcagc cacgggcaaa tgtgggcaat
    26581 cctgccgcaa acctggctgg gtaacctggt cggttccgtc ttcgttgcca tgctctatag
    26641 ctggggcggc ggtagcctgc tgccggtaga taccagcatc gttcactccg tcgcgctggc
    26701 taaaaccact gcaccggcaa tggtactgtt cttcaaaggt gcattgtgta actggctggt
    26761 ttgcctggca atctggatgg cgctgcgcac tgaaggggcg gcgaaattta tcgctatctg
    26821 gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac tctatcgcta acatgacgct
    26881 gttcgcgctc tcctggttcg gcaaccacag cgaagcctac acgctggcgg gtattggtca
    26941 taacctgctg tgggtgacgc tgggtaatac tttatcaggt gccgtattca tgggattggg
    27001 ttattggtat gctacgccga aagcgaatcg tccggttgcg gacaaattta atcaaactga
    27061 aacggctgcc ggttaattac taaggggttt ttacgtggat catttgccta tattttgcca
    27121 attacgcgat cgcgactgtc tgattgtcgg cggtggtgat gtcgcggaac gcaaagcaag
    27181 gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca ttagcgttta ttccacagtt
    27241 caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa gggccatttg atgaaagcct
    27301 tctcgacacc tgctggctgg cgattgcagc gacggatgat gacacgctta accagcgcgt
    27361 cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgta gtcgatgcgc cgaaagccgc
    27421 cagctttatt atgccgtcga ttattgaccg ctcaccgcta atggtagcgg tctcctctgg
    27481 cggcacctct ccggttctgg cgcgcctgtt gcgcgaaaaa ctcgaatcac tgctgccgtt
    27541 gcatctgggc caggtggcga aatatgccgg gcaattacgc gggcgagtga aacaacagtt
    27601 cgccacgatg agtgagcgtc gccgtttctg ggagaaattg ttcgttaacg accgcctggc
    27661 gcagtcgctg gcaaacaacg atcagaaagc cattactgaa acgaccgaac agttaatcaa
    27721 cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt gcaggtccgg gcgatgccgg
    27781 gctgctgacg ctgaaaggac tgcaacaaat tcagcaggca gatgtggtgg tctacgaccg
    27841 tctggtttct gacgatatta tgaatctggt acgccgcgat gctgatcgcg ttttcgtcgg
    27901 caaacgcgcg ggataccact gtgtaccgca ggaagagatt aaccagatcc tgctgcggga
    27961 agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc gatccgttta tttttggccg
    28021 tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt ccgttctcgg tggttccggg
    28081 tattaccgca gcttctggtt gctctgccta ttcgggtatt ccgctcacgc atcgcgatta
    28141 tgcccagagc gtgcgcttaa ttaccggaca cttaaaaacc ggtggcgagc tggactggga
    28201 aaacctggcg gcagaaaaac agacgctggt gttctatatg gggctgaatc aggctgcgac
    28261 tattcagcaa aagctgattg aacacggtat gcctggcgaa atgccggtgg caattgtcga
    28321 aaacggaaca gcagtcacgc agcgcgtgat tgacggtacg ctcacgcaac tcggtgaact
    28381 tgctcagcaa atgaacagtc catcgctaat tattattggt cgggttgttg gcctgcgcga
    28441 taaactgaac tggttctcca accattaatt taacccgggc cagagaattc tggccttctt
    28501 aatgactctt tttatatatt cagcaaataa aacatagccc ctta
//
LOCUS       DAZKGS010000053        28304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKGS010000053 DAZKGS010000000
VERSION     DAZKGS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..152
                     /gene="tnaC"
                     /locus_tag="ACHOQY_002188"
     CDS             78..152
                     /gene="tnaC"
                     /locus_tag="ACHOQY_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9678279.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            373..1788
                     /gene="tnaA"
                     /locus_tag="ACHOQY_002189"
     CDS             373..1788
                     /gene="tnaA"
                     /locus_tag="ACHOQY_002189"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9678280.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1879..3126
                     /gene="tnaB"
                     /locus_tag="ACHOQY_002190"
     CDS             1879..3126
                     /gene="tnaB"
                     /locus_tag="ACHOQY_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9678281.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3258..4433
                     /gene="mdtL"
                     /locus_tag="ACHOQY_002191"
     CDS             3258..4433
                     /gene="mdtL"
                     /locus_tag="ACHOQY_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9678282.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4408..5367
                     /gene="yidZ"
                     /locus_tag="ACHOQY_002192"
     CDS             4408..5367
                     /gene="yidZ"
                     /locus_tag="ACHOQY_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9678283.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5524..6273
                     /gene="yieE"
                     /locus_tag="ACHOQY_002193"
     CDS             5524..6273
                     /gene="yieE"
                     /locus_tag="ACHOQY_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9678284.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6295..6861
                     /gene="yieF"
                     /locus_tag="ACHOQY_002194"
                     /gene_synonym="chrR"
     CDS             6295..6861
                     /gene="yieF"
                     /locus_tag="ACHOQY_002194"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP9678285.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6915..8252)
                     /gene="adeP"
                     /locus_tag="ACHOQY_002195"
     CDS             complement(6915..8252)
                     /gene="adeP"
                     /locus_tag="ACHOQY_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9678286.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8419..9084
                     /gene="yieH"
                     /locus_tag="ACHOQY_002196"
     CDS             8419..9084
                     /gene="yieH"
                     /locus_tag="ACHOQY_002196"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9678287.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9151..9624
                     /locus_tag="ACHOQY_002197"
     CDS             9151..9624
                     /locus_tag="ACHOQY_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678288.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9673..10260
                     /gene="cbrC"
                     /locus_tag="ACHOQY_002198"
     CDS             9673..10260
                     /gene="cbrC"
                     /locus_tag="ACHOQY_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFP9678289.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10322..11044)
                     /gene="yieK"
                     /locus_tag="ACHOQY_002199"
     CDS             complement(10322..11044)
                     /gene="yieK"
                     /locus_tag="ACHOQY_002199"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9678290.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(11059..12228)
                     /gene="yieL"
                     /locus_tag="ACHOQY_002200"
     CDS             complement(11059..12228)
                     /gene="yieL"
                     /locus_tag="ACHOQY_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9678291.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12255..13871)
                     /gene="bglH"
                     /locus_tag="ACHOQY_002201"
     CDS             complement(12255..13871)
                     /gene="bglH"
                     /locus_tag="ACHOQY_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP9678292.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13957..15351)
                     /gene="bglB"
                     /locus_tag="ACHOQY_002202"
     CDS             complement(13957..15351)
                     /gene="bglB"
                     /locus_tag="ACHOQY_002202"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP9678293.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15370..17247)
                     /gene="bglF"
                     /locus_tag="ACHOQY_002203"
     CDS             complement(15370..17247)
                     /gene="bglF"
                     /locus_tag="ACHOQY_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP9678294.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17381..18217)
                     /gene="bglG"
                     /locus_tag="ACHOQY_002204"
     CDS             complement(17381..18217)
                     /gene="bglG"
                     /locus_tag="ACHOQY_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP9678295.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18503..19228)
                     /gene="phoU"
                     /locus_tag="ACHOQY_002205"
     CDS             complement(18503..19228)
                     /gene="phoU"
                     /locus_tag="ACHOQY_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9678296.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19243..20016)
                     /gene="pstB"
                     /locus_tag="ACHOQY_002206"
     CDS             complement(19243..20016)
                     /gene="pstB"
                     /locus_tag="ACHOQY_002206"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9678297.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20107..20997)
                     /gene="pstA"
                     /locus_tag="ACHOQY_002207"
     CDS             complement(20107..20997)
                     /gene="pstA"
                     /locus_tag="ACHOQY_002207"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9678298.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20997..21956)
                     /gene="pstC"
                     /locus_tag="ACHOQY_002208"
     CDS             complement(20997..21956)
                     /gene="pstC"
                     /locus_tag="ACHOQY_002208"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9678299.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(22043..23083)
                     /gene="pstS"
                     /locus_tag="ACHOQY_002209"
     CDS             complement(22043..23083)
                     /gene="pstS"
                     /locus_tag="ACHOQY_002209"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9678300.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23331..24404)
                     /gene="lpfD-O113"
                     /locus_tag="ACHOQY_002210"
     CDS             complement(23331..24404)
                     /gene="lpfD-O113"
                     /locus_tag="ACHOQY_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP9678301.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            complement(24415..26937)
                     /gene="lpfC-O113"
                     /locus_tag="ACHOQY_002211"
     CDS             complement(24415..26937)
                     /gene="lpfC-O113"
                     /locus_tag="ACHOQY_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP9678302.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(26962..27693)
                     /gene="lpfB-O113"
                     /locus_tag="ACHOQY_002212"
     CDS             complement(26962..27693)
                     /gene="lpfB-O113"
                     /locus_tag="ACHOQY_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP9678303.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(27741..>28304)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOQY_002213"
     CDS             complement(27741..>28304)
                     /gene="lpfA-O113"
                     /locus_tag="ACHOQY_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP9678304.1"
                     /translation="NIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTAD
                     QIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVG
                     TPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFI
                     ARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         7224 a         7095 c         6604 g         7381 t
ORIGIN      
        1 catttaaaca attttagaat agacaaaatt cttgagtatt atactgtagc ctcgtgtctt
       61 tcgaggataa gtgcattatg aatatcttac atatatgtgt gacctcaaaa tggttcaata
      121 tcgacaataa gattgtcgat caccgccctt aatttaccct tctgtagcca tcaccagagc
      181 caaaccgatt agattcaatg tgaacgatta gtttgctata tcttaatttt gccttttgca
      241 aaggccatct ctcgtttatt tacttatttt agtaaatgat ggtgcttgta tatatacatg
      301 gcgaattaat ggatattgca gatgtaatat tcacagggat cactgtaatt aaaataaatg
      361 aaggattatg taatggaaaa ctttaaacat ctccctgaac cgttccgcat tcgtgttatt
      421 gagccagtaa aacgtaccac tcgcgcttat cgtgaagagg caattattaa atccggtatg
      481 aacccattcc tgctggatag cgaagatgtg tttatcgatt tactgaccga cagcggcacc
      541 ggggcggtga cgcagagtat gcaggccgcg atgatgcgcg gcgacgaagc ctacagcggc
      601 agccgcagct actatgcgtt agccgagtca gtgaaaaata tctttggtta tcaatacacc
      661 attccgactc accagggccg tggcgcagag caaatttata ttccggtact gattaaaaaa
      721 cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg cattctctaa ctatttcttt
      781 gataccacgc agggccatag ccagattaac ggctgtaccg tgcgtaacgt ctatatcaaa
      841 gaagccttcg atactggcgt gcgttacgac tttaaaggta actttgacct tgagggatta
      901 gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt atatcgttgc aaccatcacc
      961 agtaactctg caggtggtca gccggtttca ctggcaaact taaaagcgat gtacagcatc
     1021 gcgaagaaat acgatattcc ggtggtaatg gactccgcac gctttgctga aaacgcctat
     1081 ttcatcaagc agcgtgaagc agaatacaaa gactggacca tcgagcagat cacccgcgaa
     1141 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtaccgatg
     1201 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
     1261 ctttgcgtgg tgcaggaagg gttcccgaca tatggcggcc tggaaggcgg cgcgatggag
     1321 cgtctggcgg taggtctgta tgacggcatg agtctcgact ggctggctta tcgtatcgcg
     1381 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
     1441 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccgc atatcccggc agaccagttc
     1501 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
     1561 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
     1621 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
     1681 gaagccttta aacatgtgaa agagaacgcg gcgaatatta aaggattaac ctttacctac
     1741 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
     1801 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
     1861 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
     1921 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
     1981 gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt ttcaatgctt
     2041 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
     2101 accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat taccgttgcc
     2161 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
     2221 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
     2281 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
     2341 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
     2401 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
     2461 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
     2521 ctgattattt gctatggaaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
     2581 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
     2641 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
     2701 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
     2761 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
     2821 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
     2881 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccgggctg
     2941 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
     3001 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
     3061 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
     3121 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
     3181 tgtccaaatg gcaactcgcc tgattctcct tcaccacgta tgctttgcgt caccttacta
     3241 tcaggacgct ttagcccatg tcccgctttt taatttgtag ttttgccctg gttttacttt
     3301 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
     3361 ccagcgaagc gcagttgcat attgcgttct ccgtttatct ggcgggcatg gcagctgcga
     3421 tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gcctgtcgcc atccccggcg
     3481 cggcgctatt tattatcgct tcggtgttct gttcactggc tgaaaccagc acgttatttc
     3541 ttgcagggcg ctttctacag gggttgggcg caggctgttg ttacgtggtg gcgttcgcca
     3601 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcgtta ctcaacggta
     3661 ttacctgcat cattccggtg ttagcgccgg tgctcggaca cctgattatg cttaaattcc
     3721 cttggcagag tctgttctgg acgatggcaa tgatgggcat cgcggtactg atgttatctt
     3781 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
     3841 atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg
     3901 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatgggtt
     3961 ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc atgaccgttt
     4021 cattctccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcgc
     4081 aggtgttatt ccttgcggcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
     4141 tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
     4201 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
     4261 aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga
     4321 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttactg attatgttcg
     4381 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
     4441 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
     4501 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
     4561 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
     4621 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
     4681 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
     4741 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
     4801 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
     4861 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
     4921 tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg
     4981 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
     5041 cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
     5101 gctatgagcc tgccggaatt tgagcagtca ctgtttatgg cagcgcaacc cgacaatctg
     5161 ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg
     5221 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc
     5281 ctgctgtggc ataaacggaa cagccataat ccgaagatcg tctggttacg ggaaaccatt
     5341 aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
     5401 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
     5461 tccatcattc cagtaaatga ttactcttat attcataatg gaccattaag catggagcga
     5521 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
     5581 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
     5641 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
     5701 ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg
     5761 ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt
     5821 ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat
     5881 aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa
     5941 gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg
     6001 aatgacgatc cgcgcgatct gcagctgctg cccattgccg gacgcctgaa atgtgctcat
     6061 gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tagtgtggtc cgtggcggtc
     6121 acgcccgcga ttgaaaagct cagtgtctgg gagttagatg gcaaacacag ctggaaaagc
     6181 ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag
     6241 ctctctaccg tgaagtcttt ttcgccaaat tgatagacaa ccacaggagt cattatgtct
     6301 gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg
     6361 gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aagtcaatgc gttaccttcc
     6421 attgccgaca ttcccctgta tgacgctgac gtacagcagg aagaaggttt tccagcaacg
     6481 gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa
     6541 tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg
     6601 gatcaaccgc tggccggtaa accggtgttg attcagacca gctcaatggg cgtgattggc
     6661 ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg
     6721 aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatccgca aaccggagaa
     6781 gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt
     6841 attcagcgag ttaagatcta aataaaaaac ccgccagcaa tcatgcatgg cgggttttta
     6901 acgcgctatc gattttagtg agcgtcgata aagacaatct tcaggataaa cagcagcgca
     6961 acgatgatta cgcacgggct tagatcacgc agacgtccgg taccgatttt catcacgcag
     7021 taggagataa agcccagcgc gataccttcg gtaatcgaga agctgaacgg catcatcacg
     7081 gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg tgccagactg
     7141 gaagtcatca gcacgccaac gtaaatcagc gcaccagctg cagcgtagcc tggcaccatc
     7201 cccgccagcg gcgacagaaa gataaccagc aggaacagca gaccaacaac cactgccgtc
     7261 agaccggtac gaccgccaac cgatacgccg gaagaggact caatataagc cgtaacggaa
     7321 gaagtaccga taaacgaacc ggtcacggaa gagatactgt cgacatacag cgcctgcttc
     7381 atgcgcggga atttaccctt ctcatccgcc aggcctgctt tatcggtcac gccaatcagc
     7441 gtaccggagg agtcaaacag gttgaccagc atgaaagaga aaatcacccc tgccagcccg
     7501 aggttaaacg acccggctaa atctacatga cccacaactg tcattacgct cggcggcgca
     7561 gaaacgatgc cattgtagtg cacatcaccc agcatccagc ccagcagcgt cgtcaccacg
     7621 atagaaacca gcaccgctgc gtgaatgttg cgcgaggcca gaatagcaat gatgaagaag
     7681 ccgaggatac ccagaagtac gctgtgagaa gtcagattac cgatgctcac cagcgtttcc
     7741 gggttagcga caatcacacc tgcgtttttc agccccatca tgccaatgaa cagaccgata
     7801 ccgctggtaa tacccacacg cagactcacc ggaatgttgg caatcatcca gtagcgaacg
     7861 cggaaaatcg tcagtaacag cagacctatc gcgccccaga agattgcgcc catcccgacc
     7921 tgccacggca agcccatcgc ctgtacaacg acaaaagcga agaacgcatt caggcccata
     7981 gcgggtgcca gtgcaactgg caggttagca aacagtccca tcataatact gccgaatgca
     8041 gcgatcagac aggtagtgac gaagacggcg ctggtatcca tgccagcaac gccaagaatt
     8101 tgcgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact
     8161 tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg
     8221 ccagaagcct gggttgtgtg ttgttgactc attatctatt tccgaacaag gagggaaaat
     8281 ccgtcgctat cgtataccaa aatgagacaa tatggacggc tgaagataac tttttttcat
     8341 tgtatttgct tatacggcaa cgattgcgtc atgcgaattg gcatgacgaa aacgttaaac
     8401 taacgataaa ggaaagacat gtcccagata gaagcggtat ttttcgactg cgacggtacg
     8461 ctggtcgaca gtgaagtcat ttgctcccgc gcatatgtaa cgatgtttca ggaatttggt
     8521 attacgctcg atcctgaaga ggtattcaaa cgtttcaaag gtgtaaaact gtacgaaatt
     8581 atcgatattg tttcccttga acatggtgtt acgttagcga aaacagaagc tgaacacgtt
     8641 taccgtgcag aagtcgctcg gctgttcgat tcagaactgg aagccatcga aggggctgga
     8701 gcgctcctgt cagcgatcac tgcgccaatg tgtgtggtat ctaacggccc aaataacaaa
     8761 atgcagcatt ctatgggcaa gctgaatatg ttgcactact tcccggataa actgttcagc
     8821 ggctacgata ttcagcgctg gaagccagac ccggcgttaa tgttccatgc ggcaaaagcg
     8881 atgaatgtaa atgtagaaaa ctgcattctg gttgatgact cagttgccgg tgcgcaatct
     8941 ggtatcgacg caggtatgga agtgttctac ttctgcgccg acccgcacaa taagccgatc
     9001 gttcacccga aagtcaccac ctttacccat ctttcgcagt taccggaact gtggaaagcg
     9061 cgtggttggg atattacggc atagttcttc acactccctt cacttacccc gcttaaattg
     9121 gcgctcaaag gtaagtaaag ggagtttgat atgtctgttt cacgtcgggt aatacatcac
     9181 ggactttatt ttgcggtttt aggaccgtta attggtgttc tgtttcttgt cctctacata
     9241 ttcttcgcaa aagaaccgct ggttcttttg cgaagaatac aggtacttcc attgtttctc
     9301 ttattgtcga ttactacggg agttattcct gcattgttaa ccggcgtgat ggtcgcctgt
     9361 ctgccggaaa agatcgggtc acagaaaaat tatcgttgtc tggctggtgg cataggtggc
     9421 gtcgttatca ccgagatcta ttgtgcagtt attgtacata ttaagggcat ggcttcctcg
     9481 gagttgtatg aaaacattct ttctggtgac agtctcgttg tccgcatcat tcctgcattg
     9541 ctggcaggtg tggtgatgag cagaatcatt acccgtctgc ccggattgga tatttcatgt
     9601 cctgaaacag actctttaag ttaagcggga tactttatct ttgggctact caaaagcaga
     9661 caggatgtta ctatgactca aaatatcagg ccgttacccc aattcaaata tcatcccaag
     9721 ccactggaaa caggcgcatt tgaacaggat aaaaccgtag agtgcgattg ctgtgaacaa
     9781 cagacgtcag tttattactc gggtcccttt tattgcgttg atgaagttga acatctctgt
     9841 ccgtggtgta ttgcggacgg ttctgctgct gaaaagtttg caggtagttt tcaggatgat
     9901 gccagcatag aaggtgttga atttgagtat gatgaagagg acgaatttgc cggtattaag
     9961 aacacatatc ctgatgaaat gctgaaagag ttggttgaac gcacgccagg ttatcatgga
    10021 tggcaacagg aattctggct tgcgcattgt ggcgatttct gtgcttttat cggctatgtg
    10081 ggctggaatg atataaaaga tcgcctcgat gaatttgcca accttgaaga agattgtgag
    10141 aatttcggta ttagaaattc tgatctagct aaatgcctgc aaaagggtgg tgattgtcag
    10201 ggttatctct tccgctgtct ccactgcggc aagctgagac tgtggggtga tttttcgtag
    10261 ttatttaaat aatgagaaca ggccggagcg taattcatac atccggcctt atttcttcag
    10321 cttaatcgag cgctaattct gcggcagcgg ctttatcagc gattaccatc agcgaaggat
    10381 gcagctgcaa cacggaagct ggaacatctt cggtaaccgg accttgcagc acatttttca
    10441 gtgcctgcgc tttacctgcg ccactgacaa ttatcagcaa gtttttcgcc gccatgatgc
    10501 ttttcggccc catcgtcacg tagctgtcag gcactaacga aaagtcgccg cccaactcac
    10561 catgcgccac aatatcaacc atctctccct ggatcgggaa ctccacggtt tgctcatgga
    10621 aatgggtcgt attcggcaaa ttgccacaaa aatgaccatc tgcacctaat cccagcacca
    10681 ccaaatccag gccgccttca cgcgccagtt tctgatcatg ctcgcggtag ttatcaatag
    10741 tgagcttctg gatattctct tctttgatcc ccgcaggggt gaaaaacaga ttacgcagat
    10801 tggtaatcgt tacaccttct ccctctttgc cgcgaaaggg aatttcatcg aaattataga
    10861 aatagcagtt atcataccag ggcttacctt taaccagggt ggtgaggtat tcatacatgc
    10921 ctttgggcgt gctaccggcg gtaattgcca ggttaacacg acgcgtcttc gacatataac
    10981 caagcagatg gtgtgccgcg acacggctca tttcctggta atcttcggta atgattaatt
    11041 tcatcttagt gccttatttt atttaaataa tttctgtacg aaggctgcat aggcaggccg
    11101 ccagacatcc atttcatggt tcaggccggg atattcctgg taatcaaact taattttttt
    11161 ctgctcaagc tcagttttca gcccggcgat atccttgccg gttacgacat ctttatcacc
    11221 aacgaccaca gtaaaattac gtagttgctg gttaatagct gccggatcgt tcagccgggc
    11281 cgcgacacct tcatccggta cggttgtcgt ggtaacacca ctgaatgtgg ccagccagcc
    11341 aaagctttcc agatgattca ttccggaaac cagcgcctgg tacccgcctt gtgaaagccc
    11401 tgccagcgcg cgaccatcgg catctttacg gacattaaaa cgcttgctaa tcagcgggat
    11461 aatatcgttc atcagttcgc gatctgccgc tttagcattc agcggataaa agactttacg
    11521 tctttcctga ggcacgaaat cttcgggaat aatgcccttc gcatcggttt cggtatccgg
    11581 gatcaccacc agcatcggtt taattttccc ttcagccagc aggttatcca tgatttgcgg
    11641 gatacgcccc tgatcgatag cggaacgtcc ggtatcacca aaaccgtgat agaaatagag
    11701 cactggcaaa ggctcgccca tgccggtgta acctggcggg gtccagacat acatctgacg
    11761 ttcagattgc aatgcgttgg agtggtaagt tatggcgatc aaatcaccat gcgcaacaga
    11821 acgcgtatcc agataactgc ctggcaccag aatcatactg gagttaacct ggcgctgagg
    11881 cttagtcatt gcggtgcctg tatcaatgct gcgtacacca tcaacattga aaaaatactc
    11941 gtacaggttg cctttcagga taggtgtgcg ccacgaccag actcctgctt cgtctttggt
    12001 catcgggtga atattgtccg gaaccggaac acccaccact acagagacat tttttgcccc
    12061 aggggcaaag tagcgaaagg tcacgctgtt atcggcattg acctgagtca catactgttt
    12121 caccgggata gtgggtgccg ggctggctgg catatccgct gctatggccc actgagcaga
    12181 aataccgctg gcaatagcca gcgttaaagc agcaattttt atattcatcg taatattcct
    12241 ttcattaagt tccattacca ccagatttca gcctgggcac cgacagcgaa ctggtcgtct
    12301 ttattgtctt cgaaggtgaa gccatccagt tcgttttcca gggctttgat atacgtggcg
    12361 tagaaacgaa tttccggacg cgaggtcaac atactggtat tgactttaaa ggtatggaag
    12421 agcgtggttt tataaccaga ctcattaaat ttattactat tcgcatcttt gttttgctgg
    12481 gtgaaatagc ccagttcaac acctgtctgg ttatattggt cccaaatata ggccgggcga
    12541 acaaccgcac gaatagattc gaaatcagag tgtgcgcctg tttcgtagct ataaatatcg
    12601 ttaccgaagg agtaaacaat cgcgttagcc acaataaaat gatcgccgat ataggcttcg
    12661 ccctgtgaag tcagacgaac tgctgttccg ccggtgtgat caccataata acgaccatta
    12721 aaggtggtaa atggacttgc gccagcataa cggccaaagt tactggcgat agagttattc
    12781 gcaaccagga aggagaattc gttgaagcca cctttatcaa atttctgcgt taaagatgtg
    12841 ccaaacatcc aggtatcttt ccagtcataa tagccgttat tatcctgatt atctttttcc
    12901 gatgcgcttt cgtttgccgt gacataacga ccactcacca ttaaggtcgc tttatcccat
    12961 aacgggatat ctttatagcg taaatcaata gtattggtat taatctgctg tttgttttgc
    13021 aggctgcgat cgtaatcatc gacatcttcg cgaaccagcg cgatatcaat tttacccgga
    13081 ccgactttcc agttttccag acctacaccc gctgcggcat cagtacgctg cgttttccag
    13141 tccagcatct ggatttcaat tttcggcgca ccgtgtttac ccacccagaa atcagcctct
    13201 ggcgcaaagg gcaggaaacc tttggtggta acgtacatat cggagaactg cataaagttc
    13261 tcgccaccgg cgttatcgcc aaaccagccg gtagagtact gctgaccaac gttaccatcc
    13321 atcatcacaa cggcatcaac ccgtttgccg ttttcgttgt agacacgttg ttttaactgc
    13381 aaatcaaacc agccggagta ttcgttacca aagcggccca gagaaccaat cgcccatgat
    13441 ttaggtgaac catgagaggc ggtcccccaa ccagaacggt aatagccgtt gtaactaaag
    13501 ccaatttcat ctttcacaaa cttactgaaa tctttcagag tcatggaaga ataaatcgag
    13561 cctgtctcac tggcattttt ctcttcattt ttgaccagta cagcatcagg ttttgcggca
    13621 ctactggtcg ggaacggatt ggcggcatac ccttgatcat tcgtacttac gctacgattc
    13681 accgtcgctg gcgtatattt tttcttctct tcatctttat actttttgag ttcgctttgc
    13741 gtttctctta acgctttttc taataactca aggcgatgtt ccaccgttaa tgattcagcc
    13801 aatgattgtg cagaaaaggc taacggtgcc attaataaga tggcagaggt aataagattt
    13861 cgtctaaaca taatatccct ttatggtgca aagaaagaat taacgtatcg catccagact
    13921 gttctgaatg cgacgataat taaggtgctt tattggttat ttttttaatg acagcccccg
    13981 cgttttgatc acttctgcat accatccgaa gcttttctta cgcgttcttg tgaggcttcc
    14041 ttcgccatta tcatcacgat ccacataaat aaagccgtag cgcttagaca tttgtgaatg
    14101 agatgcactg actaaatcaa ttggccccca actggtgtac cccataatat ccacaccatc
    14161 ggcaatcgct tcatttacct gtaccaggtg atcgtttaaa taggcaattc gataatcgtc
    14221 ctgtatcgaa ccatccgctt caacgctgtc ttttgcgcct aatccgttct cgacaataaa
    14281 taacggtttt tgataacgat cccaaagcgt atttaacaga acccgtaatc caaccgaatc
    14341 aatttgccac ccccactctg aacttttcag atgcggattg gggatcatat tcagtatgtt
    14401 gccctgcgca tttttattaa tgctttcgtc gtgggaaaca caaccagtca tgtaataact
    14461 aaaagagatg aaatcgacgg tatgttttaa atcttctgcg tcactttcag tcatctcaat
    14521 ggtgatattg tggtcgcgga agaaacgctg catatagccg ggatactggc cacgcgcctg
    14581 aacatcacca aagaacatcc agcgccggtt ctcttccatg gcctgcaaca tatcctgtgg
    14641 ctggcaggtg agggggtaaa ccagcccacc gagaagcata ttgccgattt tcgcttcggg
    14701 gagcaggcta tgacaggctt taactgcccg cgcactggca accagttgat ggtggatagc
    14761 ctgataaact tccgcctcgc cactctcttc tgccagcccc acgcccgtga atggctcgtg
    14821 taacgacatg ttgatttcat taaacgtcag ccataacgcc actttatgtt ggtagcgagt
    14881 aaagaccgtg cgggcgtaat gctcgaagtg atcgatgacc gctcgattag cccaaccgcc
    14941 gtagtttttc accagcccat atggcatttc gtaatgggat aacgttacca gcggcttgat
    15001 ccccgcctgc gccatttcat caaacagccg atcgtaaaac gctaaccccg cttcattcgg
    15061 ttcgacttcg tcgccctgag ggaaaattcg cgcccaggca atggaaatac gcagacaggt
    15121 gaagcccatc tcggcaaata acgcgatatc ttccgggtaa cggtgataaa aatcgatggc
    15181 gacatctttg atattctctt tccccaggat acgcggttcc atttttccca ttacgccatg
    15241 aggctgtaaa tctgaggtcg agatcccttt gccatcttcc tgccaggcac cttccacctg
    15301 attggcagct gttgcgcccc cccaaagaaa tgtttctgga aatgctttca taattaactc
    15361 cttttatcgt tagcgaatga tggataacag cggttcacct gcgcttatct gcgccgtgcc
    15421 gtggggtaat acgtccgtaa aatcatcgct attactgatt aataccggcg tcgtcagatc
    15481 aaatccggcc tcgcgaatag cagggatatc aaaagaaatc agccgatcgc ctgtattgac
    15541 cttgtcaccc acgttgacgt gagcggaaaa gaatttgccg tccagtttta cggtgtcgat
    15601 accgacatga atcaggagct ccacaccatc atctgactca atgccaatgg cgtgtaatgt
    15661 ggcgaacaac gaagcaattc gacccgcaac cggagaacgc acttcaccaa ccgagggcag
    15721 aatggcgata cctttaccca acaggccact ggcaaacgtg gtatcagcga cgtgaatgag
    15781 cggcacaatc tctcccgtca tcggtgaaca gataccgccc tgctcaggtg gtgtaataac
    15841 ctctggtgtt ttctcttgcg gggcaccctg cgctggctga cgtttagcgg tgatgaaatg
    15901 aagcatcacc gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa taacgctggc
    15961 ccagacggtg aaatcaattc ccgttgacgg gatggtttgc atgaaggtga aaatacttgg
    16021 caaaccaaag gagtacactt tcgtttgcgc gtagccaatg atggtggccc ccaaagcccc
    16081 actgatacag gcgataacaa aggggtactt acgcggcaga ttaacgccat ataccgctgg
    16141 ttcggtgata ccaaacaggc ccgtcaacgc cgctgatccc gccaccactt ttttctgcgc
    16201 atcgcgttcg cagaggaaga cgccgagcgc cgccccgacc tgcgccataa tggcgggcat
    16261 taacagcggg atcatggtgt cgtagcccag cacggtgaag ttattgatac acagcggcac
    16321 caggccccag tgcagtccga acatgacgaa gatttgccag aagccgccca ttaccgcgcc
    16381 tgcaaatgca ggaaccgcct gataaagcca gagataaccg gcggcaatca gttcgcttat
    16441 ccaggttgat agcggcccca ccagcagaaa ggtgacgggt gtgataacca tcagacatag
    16501 caatggtgtg aagaaatttt tgattgccga cggtaaccac gcattaagtc ggcgttccag
    16561 aatgctgcac aaccaggcag aaaaaataat gggaataacc gatgacgagt aattcaacaa
    16621 tgtgaccgga atacccagga aatccagccc cagctcatcc gctttttgcc cgttctcgaa
    16681 agcagtcaga attaatggat gcactaacgc tccaccaatc accatggcag taaatggatt
    16741 accgccgaag cgtttccccg cggtgtatcc caggattatc gggaagaacc aaaacaaggc
    16801 atcactggcg ctgaataaaa ttaaataagt accactttgt tcggtcgtcc actgaaaagt
    16861 gagcgccaga gccagcatac ctttcaggat cccggttgcc gccatcaaac cgatcagagg
    16921 cgtaaaaata cctgaaataa cataaacaaa gcggtttagc agattacctt tctcatcatt
    16981 ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg ctgttgaccg ccaggaagac
    17041 atcggccaca tggttaccta tgaccacctg aaactgacca ccgctttcca ccaccataat
    17101 aataccgggg gtctttttca gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa
    17161 acgtaatcgc gttgcgcaat gcatcagact cacaatgtta tctgcgcccc cgactcctgc
    17221 gactattttt ctggctaact ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc
    17281 aaaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc aggttttgcc
    17341 cgcttagtgc ggtaacaatc ctttactcag taataatatt tcagtgttct ttgcgcacgc
    17401 gctctatatt tatggctaaa aacataatct ctgcgggtga aattttacgt tgatactgca
    17461 aaccaataaa aatggcgatc cgttccgcac attgccatgc ttgcgggtaa ttttgtttta
    17521 ctgcttgttg taatgattca tcactatcgt taattgaagc atgttcaaga atacgccagg
    17581 ataaaaactt cagatgtgta accagtcgct gataactcaa gctttcttcc tggtaattaa
    17641 ggctgaactg aaattttatt aattgcagca tttcgcgcat taactgcgtg acacctgcaa
    17701 catcctccat atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt
    17761 catctttcgg taactgcacg cccaaccgtt tatcaataat ggttaacgct tcttccccta
    17821 gctggaactc tttcgggtaa agccgctgga tatcccacag caacgggttg ggcagcaaca
    17881 cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac
    17941 tgtcctgtaa ttttcccaag cgctcctgcg ctaaagagat aatacgatca caggttgcca
    18001 tcacctcaag aggaatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc
    18061 tcaaggcata ctctttttct attccacttg agttaattct ttcgccagcg cgtttttgaa
    18121 agccaattcc tcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa
    18181 cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataaaca
    18241 aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
    18301 acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga aattaattta
    18361 attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta
    18421 ttaactttgt gtaattttag gaatttataa agttatatat aacaaatccc aataattaag
    18481 ttattgggat ttgtctggtg aattatttgt cgctatcttt tcccgccagc agtttatcca
    18541 gctcatcacc gcctacatga cggaaatcct gccccttcac gtagtagaag ataaactcac
    18601 aaatattctg gcagcggtcg ccaatacgtt cgatagaacg cgcgcagaac agcgcagtaa
    18661 gtacgctcgg aatggtacgc gaatcttcca tcatgtaggt catcagttga cgaacaatac
    18721 cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
    18781 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
    18841 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
    18901 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
    18961 taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt
    19021 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca
    19081 gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc
    19141 cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa
    19201 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
    19261 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
    19321 ctcaatcaat tcgcccaggt acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg
    19381 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc
    19441 aatacgcccg gtagagatag ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc
    19501 cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga
    19561 gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
    19621 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa
    19681 agcgatgttg tcgtagatgg acatcggaaa cggcgtcggt ttctggaaca ccatgcccac
    19741 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag
    19801 aatttcacct tccgcacgct gctccgggta cagttcaaac attttgttga aggtacgcag
    19861 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gttacctggt ttttagcgat
    19921 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
    19981 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtgcct
    20041 gatgcacttc gctaatcagg cttaccgtag gccggatcag gcgttcacgc cgcatccggc
    20101 aaagtgttaa ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag
    20161 tacgcacagg gtaatgatca ataccccggc ccaggccagt tgctgccatt ccgcaaacgg
    20221 actcatcgca aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt
    20281 gctccagaac tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cagcaatacg
    20341 ggcaatcgcc agcaagatac cggtcatgat cccggacacc gacgctttca gcgtaatcgc
    20401 ggagatcatc ttccacttcg gtgtacccag cgcataagcc gcttcacgca ggctgtccgg
    20461 caccagtttc agcatgttct cggtggtgcg gataacaatc ggcacctgca acagcgccag
    20521 cgcaattacg cccgcccagc cggagaagtg ctccatctgt gccaccacga tggtgtagac
    20581 aaacagacca accacaatcg acggcgcgga gagcagaatg tcgttaatga agcgaatcac
    20641 ttctgccagc caggatttac gaccatattc cgccagataa atccccgcca taatgcccag
    20701 cggcgtaccg aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc
    20761 cagaccacca ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat
    20821 accgtcgata ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa
    20881 ggccatcgtc gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg
    20941 cattttgcgg cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag
    21001 cgtgccccct cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg
    21061 aaggtaatca caaacaggat caggcccagt tccatcagtg ctgcaacgtg cagaccggat
    21121 tccgcttccg caaattcgtt cgccagcgca gaggtgatac tgttgcccgg catatacagc
    21181 gaggcgctgt cgagctggta ggtgttaccg atgataaagg tcaccgccat ggtttcaccg
    21241 agcgcgcggc ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga
    21301 acgatacgcc agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc
    21361 atcaccgggg tttgttcgaa cacatcacgc attaccgccg caatgtacgg aataatcatg
    21421 atggcgagga tcacgcctgc cgcgaggata ccgataccaa atgcggggcc agagaacagc
    21481 gcgccaacaa tcgggatatt cgacatgata ttgccgaccg gctcctgaaa gtaaacggcg
    21541 aacagcggcg caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc
    21601 agcagctcaa tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg
    21661 aacagggcga taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc
    21721 aacgtaccgt agatcggcac cagcgccccg tagatatcgt tcggtgcatc ccactctttg
    21781 gtccatagga aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag
    21841 acaataatgc cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg
    21901 aaaattatgt cgcccttttt acccggtggg ttaaaagcag gcttggttgc agccataagt
    21961 tactcttcag ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca
    22021 ccgtacccgg cctggagttt tattagtaca gcggcttacc gctactgtct ttaatattgg
    22081 tcttccacgc agcgcgaacc tgttcaacta cactatccgg caggctggcg taatccaggt
    22141 cgttcgcctg tttagccccg gttttgtacg cccagtcgaa gaatttcagc acttctgtgc
    22201 cttgttctgg tttcttctga tctttgtgga tcagaatgaa cgtggtagag gtaataggcc
    22261 atgcatcttc gcctttctgg ttggtcagat cctgagcgaa ggttttgctc cagtctgcac
    22321 cttttgctgc attagcgaag ttttcttcgg tcggactaac cggtttacca tcagcggaga
    22381 tcagtttggt gtacgccagg ttgttctgct tcgcgtaagc atattcaaca taaccaattg
    22441 cacccggcag acgctgaacg aacgcggcga taccgtcgtt acctttaccg cccagaccga
    22501 tcggccattt tacggtagag ccagtaccaa cgttgttttt ccactcttcg ttcactttcg
    22561 ccaggtagct ggtgaagacg aaggaagtcc cggagccatc tgcgcggcgt actacagcaa
    22621 tgttttgtga aggcagtttc agacccggat tcagtttggc gatggcttca tcatcccact
    22681 tcttgatttt gcccaggtag atgtcgccga gggttttacc atccagcacc agttcgccag
    22741 acttcagccc tggaatgtta accgccagca ccacgccgcc aatcacggtc gggaactgga
    22801 acagaccttc ctgagccagt ttttcgtcag acagcggcgc gtcagaggca ccaaaatcaa
    22861 cggtattagc gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt
    22921 tattaccggt ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg
    22981 ttgcacctgc acctgtcagg cttgcttctg caaacacaga gaaagcgctc atcgataagg
    23041 tcgcggcgac aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga
    23101 ttcataaagc attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat
    23161 atgtaaggaa tatgacattt ttatgacaga aaggaattac caaataactt actgatttta
    23221 aatgtgaaga tgaaaatccc acatgtcatc tttttgtcat gatgcattta aaagtgataa
    23281 agaaatgggt gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca
    23341 atctcaacag ttatcgtcgc tgttgcagta aattgccccg gggatgggcg agctccagta
    23401 aggctcgcag gagcagctga aaaagttaca ctacctgatt tttcaccata cactgttgat
    23461 ttacccatgt ctgttggcaa ctcaccaccg ttcactttga tttccttatt atttttgtcg
    23521 taaattttta tccccacgtt agagtttgaa gttacgacca ttgaattgtc cgtattgggt
    23581 tcggtagcat aaaaagacgt actgatatca tcggtattca gtatccccag gttttcacac
    23641 tcaatagtga ccgttttagt aattttatgt gttgttaccg cacttccaac aacagatgaa
    23701 aaatctgcag ctggtatttc tttaaaatca aaataaactg gctgtaaatt atttatttcg
    23761 caactttgcg gagctgtaat atcgccccga atataaacat cagccatcgg ctgtgagtaa
    23821 tccaccgggg tgtcacggct aattgttcca tacaatgtca ctatctttgt ttcaggaata
    23881 atcaattttc ctgcaaatgt cttcttgata taaaaatata ctatcgcttt agccccactg
    23941 gatatactta aaggttcaat gcggttgcaa ttgtactttg tattaccaga cgggttgtta
    24001 gcatgatatt caaaaggtgc attcacatac ccaaccccaa gaatatctat agcaatccca
    24061 acatcaagat tcggattaag atcataataa ttaatattag agtgaacccc agcataagtt
    24121 aatgcctggt ttatttttga cgtataatag atatagggga attctcctac gttaccagta
    24181 tcacaatgac agcgtccagg atatgaggat ccattagtta cagtggtgtt aaatgtcgtt
    24241 ccaaccttat tttcgttcgc ggtaataaga tatcccgaga aatcaagcgt actgtgaaat
    24301 acgccattat caggggtaca gataccttta ggagctgcaa gtgctacact acttaacgag
    24361 agcataaaca cagcaaagca ccactgtttg atatacttgt tcataaaaat ccttttaatg
    24421 acatgtcaca ccctgttcaa ttaatccgct ctcactttct ggtgtcgaaa gtgaataatt
    24481 gaagatgcat tttgttgagg cgttttctcc ccaggataac gtaacctgtc ctttttctgg
    24541 tagaccagaa agataaagtt cgccacggtc accgacaatt cctgaatctg ccaaacttgc
    24601 atcttgtatc gcagcaatgg ctccaaaagg taccggttta ccatttgcat ttaacactct
    24661 gaagagaacg cgataaccca catgagtcac aaactccgct ttaaccactg caccacgcgt
    24721 ggaaaccatc gtaacgacgg tattatcaag ttctatattc gacttcgaga aatagttatc
    24781 gctgattgct acccgatttt catcataagg gtttagctgt gtcttgaccg tatagccgcg
    24841 ccagtccgtc gcagcaccgc gcatattatc tatttctaac ccggcggccc caggtgcttt
    24901 aacaagagca atagtttcac ctaactcctg acttaacgtt accccttcgc tgtgtgccag
    24961 aattccccct ctgataccat aattcagcct ctgggaattt tggctgtagt tatagctgcc
    25021 gttcaccgtg ccataagagc catcataggt cagggacata ttaccgccaa cctcatcatc
    25081 atgactgtta taggactgca ttaaattcca tgaaaggtta tttcgttcca gcgccgtacc
    25141 gctgagtccg agttgatgtg agctaccgga attatcgttt tgcgaaacag aataggtggc
    25201 gtaactatta ccccaccagc ccgataaagg aacagataaa gagagcatgt agatctgctc
    25261 actggtgtta tcactggcat ggttgttccc cgtcccctgt ctggcagtat tggaataact
    25321 ccaggagacg ttccacgaga gacgcttcca gctactgctg tagccaaatt gcattaatcg
    25381 ttcgacatca tcggtatgcc agtattgttg ccggcttaat gtcaggtata aattgccata
    25441 atctctcccc aacgactgcg ttagcgttcc ctccacgcga ctacgtcggt tacctgtttc
    25501 ccaaaagtct tcagggctgt tgcgccgcga tgcccactca ttgagggtat agtagccctc
    25561 ggtggagtaa cgatagccaa ccaggcgcaa gctagtgtct gtgtcgtcaa atcgttttga
    25621 atagagaaag cgatatgact gacccgtttc tgtatcatca tggctaaaat tcgcacgagc
    25681 atgtgtcaca tcgaaagaaa ttgcgcccaa agcgccaaga tctttaccga cacccagagc
    25741 tgcagaacca taattttcag cagcctgcat acctccatat aaagtgatcc cgtgggaaaa
    25801 accatgaagt atttgcgcct gaaggacgtc cggtgtaaat ccgtcttcat cgcgcaattc
    25861 acccacgctg acatcaacat ctgtctgacc ttcacgttta agaatcgcca atgaagcata
    25921 aggttgggtg aatcgttgtt cgctaccgtc ttcttctttg atggttacgt ccaaatcgcc
    25981 acctgtactg gcggtgttga gatcggtgat ctcaaaagca ccttgtggaa cggcagactg
    26041 ataaatcacg tagccattct gccgaatgat gacggtggcg ttggactttg caatcccacg
    26101 aaccacagga gcaaagccat tctggctggc gggtagcata tcgttatcag tatacaaacg
    26161 cgcgccgcga atttgcgtac tgtcgaaaat atcgctcgcc gtccacgtat caccaatcat
    26221 aatctggctt cttaacgcgg caatatcgcg ctgtatccag gtactaatgg agttgtattc
    26281 cgcacggcca tcgctggtgt ttaaagtact ataattacgt agccgccacg gtccaatatt
    26341 cagtccattg cgcagattga gataactggt atctgtctct tgtgaaccat aatctgaacg
    26401 tagtgtatgt gaaccagaaa aatcgtagtt gatggtgagc gcgttaatcc cctcatccca
    26461 acgcgtgggg ctgatgtaat cataagccat cgcgtccatg tagatttggg gaatggatat
    26521 attcagctgc aatttatcac tgtcaaactg ccaggatgag ccttcaatac gtggagcagg
    26581 atcgaggcaa accgtctcat ccacatcatt gaacgatttg agcgcatcaa cttttatgcc
    26641 aaaggaggac agtgtttttt taatggcaat aaagcaagcg tgcagatcag catctggatt
    26701 atcctgaaca aatgtcacat cctgacgttc aaacatttta ccgttgataa aaatatcaac
    26761 gctataaaca cctgggggca tcaggtttgc ccgattaaac tggcgaatat cgactccgga
    26821 ttgatggtcg atttccagaa gcgccggatt gaatacactg acctcggcca tcaggttttg
    26881 cgagaaaatc agacacgtcc ctgccgtgag gccaaccact attctggtcg tcatcataat
    26941 acgcctatcc ttatcccttg cttacagcgt catttttatc gggtcactga ctgcgccgta
    27001 atcattaata aatcgccagg tcatttggcc tcccgcagaa ttagcaggta aaggtaacgt
    27061 gagcgtttca aaaggacgga tcatattgag gggcacatca tggccatcaa cgatgacatc
    27121 catcacactg atatgaattg ggttgggatt ctctgctatc agatttcgcc cttcccggcg
    27181 ccatttcact ttcggcgcag catcttcggg aataaggcgg atatttgctg gtcgccagaa
    27241 cagcttgata cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga
    27301 tggcggaata tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac
    27361 acctttggtg tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg
    27421 cggaataacg gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc
    27481 ctggatgaga taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt
    27541 attacttaag taaaccaccc gcgtaccacc aatgacaacg cctgcataag ttaatgctgg
    27601 ttggcctaaa aaaacgctga gtataatcgc ggtaaataac ttacatgaaa ctgttgtttt
    27661 aaagagcctg attaaacaac tttttctgga cataacgact ccctggccaa agccaactga
    27721 ataaaaaggc ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc
    27781 gccgtaccag ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca
    27841 gccttgcctt cagcatcgat agcggtataa gcagagtcgt tataaagttt aacctgcgaa
    27901 ttatcggact ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta
    27961 ccggtaaaat caccagggtt gcctgctgta agcggcgtac ccactttcag gtcgccgtcg
    28021 ccgccaggcg cttctgtacc atcaaaacga acggctgctt gagtgtacgt tgccgggcaa
    28081 ttttcaagct taatactgaa cttctgtggc gacgccagac tacctacacc tgaaaatgca
    28141 tttttactga ctttacccag tgggacaatt tgatcggctg tatcggaggt aacttcacaa
    28201 gtagtgtcga caatttcacc gacgaagtga acaacaccat cttctgccag tgcatgcccg
    28261 gccagcatta gagatgccag agtgaatgca ccgcctataa tatt
//
LOCUS       DAZKGS010000054        28150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKGS010000054 DAZKGS010000000
VERSION     DAZKGS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..1187)
                     /gene="gldA"
                     /locus_tag="ACHOQY_002214"
     CDS             complement(84..1187)
                     /gene="gldA"
                     /locus_tag="ACHOQY_002214"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9678305.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1198..1860)
                     /gene="fsa"
                     /locus_tag="ACHOQY_002215"
     CDS             complement(1198..1860)
                     /gene="fsa"
                     /locus_tag="ACHOQY_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9678306.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1872..4373)
                     /gene="ptsP"
                     /locus_tag="ACHOQY_002216"
                     /pseudo
     CDS             complement(1872..4373)
                     /gene="ptsP"
                     /locus_tag="ACHOQY_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4682..5761
                     /gene="frwC"
                     /locus_tag="ACHOQY_002217"
     CDS             4682..5761
                     /gene="frwC"
                     /locus_tag="ACHOQY_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP9678307.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5776..6096
                     /gene="frwB"
                     /locus_tag="ACHOQY_002218"
     CDS             5776..6096
                     /gene="frwB"
                     /locus_tag="ACHOQY_002218"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9678308.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6147..8440
                     /gene="pflD"
                     /locus_tag="ACHOQY_002219"
                     /pseudo
     CDS             6147..8440
                     /gene="pflD"
                     /locus_tag="ACHOQY_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            8406..9284
                     /gene="pflC"
                     /locus_tag="ACHOQY_002220"
     CDS             8406..9284
                     /gene="pflC"
                     /locus_tag="ACHOQY_002220"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9678309.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9286..9627
                     /gene="frwD"
                     /locus_tag="ACHOQY_002221"
     CDS             9286..9627
                     /gene="frwD"
                     /locus_tag="ACHOQY_002221"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9678310.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9614..10465)
                     /gene="yijO"
                     /locus_tag="ACHOQY_002222"
     CDS             complement(9614..10465)
                     /gene="yijO"
                     /locus_tag="ACHOQY_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9678311.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10614..12347)
                     /gene="eptC"
                     /locus_tag="ACHOQY_002223"
     CDS             complement(10614..12347)
                     /gene="eptC"
                     /locus_tag="ACHOQY_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9678312.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12530..15181)
                     /gene="ppc"
                     /locus_tag="ACHOQY_002224"
     CDS             complement(12530..15181)
                     /gene="ppc"
                     /locus_tag="ACHOQY_002224"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9678313.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15533..16684)
                     /gene="argE"
                     /locus_tag="ACHOQY_002225"
     CDS             complement(15533..16684)
                     /gene="argE"
                     /locus_tag="ACHOQY_002225"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9678314.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16838..17842
                     /gene="argC"
                     /locus_tag="ACHOQY_002226"
     CDS             16838..17842
                     /gene="argC"
                     /locus_tag="ACHOQY_002226"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9678315.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17850..18626
                     /gene="argB"
                     /locus_tag="ACHOQY_002227"
     CDS             17850..18626
                     /gene="argB"
                     /locus_tag="ACHOQY_002227"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9678316.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18687..20060
                     /gene="argH"
                     /locus_tag="ACHOQY_002228"
     CDS             18687..20060
                     /gene="argH"
                     /locus_tag="ACHOQY_002228"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9678317.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20327..21244
                     /gene="oxyR"
                     /locus_tag="ACHOQY_002229"
     CDS             20327..21244
                     /gene="oxyR"
                     /locus_tag="ACHOQY_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9678318.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21227..22627)
                     /gene="sthA"
                     /locus_tag="ACHOQY_002230"
     CDS             complement(21227..22627)
                     /gene="sthA"
                     /locus_tag="ACHOQY_002230"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9678319.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22958..23608
                     /gene="fabR"
                     /locus_tag="ACHOQY_002231"
     CDS             22958..23608
                     /gene="fabR"
                     /locus_tag="ACHOQY_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9678320.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23608..23967
                     /gene="yijD"
                     /locus_tag="ACHOQY_002232"
     CDS             23608..23967
                     /gene="yijD"
                     /locus_tag="ACHOQY_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9678321.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(24007..25107)
                     /gene="trmA"
                     /locus_tag="ACHOQY_002233"
     CDS             complement(24007..25107)
                     /gene="trmA"
                     /locus_tag="ACHOQY_002233"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9678322.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25221..25411
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25476..27320
                     /gene="btuB"
                     /locus_tag="ACHOQY_002234"
     CDS             25476..27320
                     /gene="btuB"
                     /locus_tag="ACHOQY_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9678323.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27265..28122
                     /gene="murI"
                     /locus_tag="ACHOQY_002235"
     CDS             27265..28122
                     /gene="murI"
                     /locus_tag="ACHOQY_002235"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9678324.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6423 a         7073 c         7771 g         6883 t
ORIGIN      
        1 gggataatac aaggcctgat aagcgtagcg catcaggcta ttttgcgttc aaactcccgg
       61 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
      121 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
      181 atggtttcac cttctgcaca tgccgcttct gccacaattc gcattttcgc cgggacatct
      241 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
      301 acggtttcga tttcctcaac cggcgcgttt tccagtacca gctgcgtcag cgtaccgaat
      361 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
      421 accgcatgag ccgccgccag tccgccgctt tcaaaaccaa cgccgcttaa ataggtgttc
      481 gcttcaatca cgcgttccag cgcaggggtt actacatgct gttcggcggc aagcatcgct
      541 ttttcgcctt cttccagcag agtgttgtag cacagttctg ccagtgccag cgccgcctgg
      601 gtgcacttgc cgcccgccat agtggtagcg ccgctacggg aacaggcgcg tgcttcaaac
      661 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggagc gcccgcgacg
      721 attttggtgt cgacaatgac catattcggg ttatttggca acagtagata gcggtcaaac
      781 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
      841 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc agtatcgagg
      901 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
      961 agacgatcga tctcattttg cgaacattca ccgccaaacg gcgcaatttc tactaccagt
     1021 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
     1081 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
     1141 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
     1201 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
     1261 taccgcaggg gtgttgagca tttgttgcgc tacatctaaa ggcagggtga tggactcaca
     1321 tcctgccagc aaacaatcca gtgcctgacg cggcgtttta aagctggctg caagcaccat
     1381 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
     1441 cccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
     1501 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
     1561 tttcagcatc ttaatggcgg ccagaccttc ggaagtcacc gggattttca ccacaatgcc
     1621 cggaatggcg tcgcgcaggc gcttcgcttc ttccaccata ccctgcgcgt cacgactcat
     1681 agtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagaacttc
     1741 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
     1801 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
     1861 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
     1921 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
     1981 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat aatgatgaat
     2041 tgcgcttcat catcgcccca gcgcaccggc gcttgcagac gcgctacact gatggtggat
     2101 tgctcaatgt gttctgattt gctgtgggga atggcaaaac taaagcccag accggtagag
     2161 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcggccc
     2221 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg tcagtcgctt
     2281 tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct gttgggtcat gcggaattgc
     2341 gccagcaggt gttccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaactgg
     2401 cggcattcgc ggctatcaag ttgcgccatt cgcgctttcg ccgccggaat tgacggtgcg
     2461 ctcatgctca gctcatccag ccctaaaccg accagcaacg gcagcacgga acctttcgct
     2521 cccagctcac cgcacagacc aatccatttg ccctggcgat gcaccgcttg cacggcgtaa
     2581 tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcatta
     2641 tcgcgatcca ccgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
     2701 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
     2761 agctggatct tctcatcaaa cggaatgtgt tcgttacgca gttgctgttt ggcttccgcc
     2821 agtttttctt tcacccataa gatctcttcc attgaagaga tcatcgggat catgattttc
     2881 aggctgccgt gagcggaggc gcgcaggatc gaccgtagtt gtgtggtgaa cagcgaggcg
     2941 tattcttcat aaatacgcac agcgcgataa ccgaggaacg ggttagcttc tgccggaatg
     3001 ttcagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
     3061 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
     3121 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
     3181 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
     3241 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcatcc
     3301 tgtacgcggg cttcttgctg ataataacgg gctacggctt cccctggctc aaccacaatc
     3361 gctccggcgt tgccgtcgat ataaatcgtt tgatgttgcc acggggtaag ggcatcaata
     3421 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
     3481 gtgccgccgc ttttgagcaa caatcctttg aggtgatttt tatccagttc gaggaactgg
     3541 ctgggggtca gttcatcagc catacaaatg gcgggctgcg tcagtttgcc cggtgccggg
     3601 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
     3661 gcacgttctt gcaggtagct gctgctggaa cgggaaaact cttcacaaaa gtgattcgca
     3721 ctggcgacaa tcgcttcggc gcagcttaat ccggcgctga cgcctgccag taaatgttcg
     3781 tgcagggaag tatcgccagc cagggatcgg tgcgcttcca gaatcgcgct ggtggcaccg
     3841 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
     3901 gcggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
     3961 aaagaagaga tcggcgtcag aatgccgcct gcgctaccgc tgcacacggt gcgggcgcgg
     4021 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
     4081 acttccgcca gcggtgcgtc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
     4141 tggtgcgctt cctgttcgtc ggccccggaa atcagtaacc ggtagttatc gcccgccagc
     4201 gtgtcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgcgcagg
     4261 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
     4321 cgtgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
     4381 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
     4441 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
     4501 acgccgtttt gcggcatgct tccggattat cgcaagttat gaggcggatc gcatttttgt
     4561 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
     4621 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
     4681 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
     4741 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
     4801 tggcaaaggc gcagtgccgg atgccgtagc cgatccgaat ctgaaaaaac tgtttgatat
     4861 cggagttgca ggtttgacgc tgatggtgcc tttcctcgcc gcttacatcg gttactccat
     4921 tgcagagcgt tctgcgctgg ccccgtgcgc tatcggtgcc tgggttggta acagctttgg
     4981 tgcgggcttc tttggtgcgc tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
     5041 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
     5101 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
     5161 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
     5221 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
     5281 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccattgctgc
     5341 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
     5401 tttctccgca gaagagcgcg aaaccggcaa agcggcgctg gtgatggggt gcgttggggt
     5461 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
     5521 ggtcggttct gtttgtggtg cggtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
     5581 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
     5641 ggctgtcgga gcagtggtga cggctgtttg cgtgaacgtg ctgaaaagtc tggcgcgtaa
     5701 aaatgggtct tcgactgatg agaaggaaga cgacctggat ttggattttg aaattaatta
     5761 attgaggaac gagccatgac gaaaattatt gcagtaaccg cgtgtccatc tggtgttgcc
     5821 catacctata tggcggcaga ggcgctggaa agcgccgcga aagcgaaagg ctgggaagtg
     5881 aaagtagaaa ctcaggggtc aattggtctg gaaaacgaac tgactgcgga agacgtggcg
     5941 agcgccgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
     6001 ggcaagacca tcgtgcgcgt caacatcagc gatgcggtaa aacgtgctga cgccattatg
     6061 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
     6121 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
     6181 tgttttccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
     6241 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
     6301 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
     6361 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
     6421 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
     6481 gcgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
     6541 tgacagatga agtaaaagcc gcgaccagca cgcagatttt cagcattaac cagacggata
     6601 aaggccaggg gcacattatt attgattacc cacgcctgct gaatcacggg ctgggggagc
     6661 tggtggcgca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
     6721 cgctgttact gctggaagcc tcgcagaaac acattctgcg ttacgctgaa ctggcggaaa
     6781 caatggcggc aaactgcaca gatgcccagc gtcgcgaaga actgctgact attgcggaga
     6841 tctcccgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
     6901 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
     6961 tcgaccagta tatgttgccg ttctatcagg cttctttaac ccagggcgaa gatccggcgt
     7021 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
     7081 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgta ctgctcggcg
     7141 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
     7201 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
     7261 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
     7321 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
     7381 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
     7441 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
     7501 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
     7561 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
     7621 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
     7681 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
     7741 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
     7801 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
     7861 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
     7921 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc ggtggttact
     7981 tcacgccggg atcgtatacc gtttctgctc acgtcccgtt gggatcggtg gttggcgcga
     8041 cgccagacgg tcgttttgcc ggggaacaac tggcagacgg cggcttgtca cctatgctgg
     8101 gccaggacgc acaagggcca acagcggtac tgaagtcagt aagctcgata atacgctgct
     8161 gtctaacggt acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg
     8221 attacgcaaa ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca
     8281 gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc
     8341 cgggctggtg gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat
     8401 ccaggatgac atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca
     8461 atgatgtggc gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc
     8521 gtacggtggt cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt
     8581 cgatctccgg caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat
     8641 gtttgcgtga tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca
     8701 gccttgacgc gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg
     8761 gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt
     8821 tacagcggct gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag
     8881 ccagcaagct gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta
     8941 tggacgcgac tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc
     9001 tgcgtttgct ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt
     9061 tcacgctcag ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca
     9121 ggcagatcca tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga
     9181 aaacatggtc gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg
     9241 aaatggcaga acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg
     9301 gcagtaaccg cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa
     9361 aagttgtgcc agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg
     9421 gagaatcgtt tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat
     9481 atcgagcttg ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac
     9541 gcattcctgc gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg
     9601 cagcaaaccc atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc
     9661 gacgatattc cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc
     9721 tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata
     9781 acgtcttagc gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg
     9841 ttttttgaaa caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt
     9901 cgcgggtaag cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg
     9961 cctggctgcg tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca
    10021 ggctggcgac gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga
    10081 gcgtttgtag cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca
    10141 gattttgata ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa
    10201 acagcacgct aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag
    10261 gaacgtacag cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac
    10321 cttccagcac aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc
    10381 cgttactgct ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga
    10441 gcagatagct gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta
    10501 actggcatta ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac
    10561 attgcggcag tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga
    10621 ttacccacct gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac
    10681 gggttaccaa tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga
    10741 tcgtaaccgt cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg
    10801 ctgtatttac gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc
    10861 gacgtccaca gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc
    10921 ccctgagttt tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc
    10981 aggaaaccgt tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg
    11041 ttatacaggt tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca
    11101 ggcggaacat gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg
    11161 tatttgatat gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc
    11221 agcacttcct ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt
    11281 tgctggttca tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg
    11341 cgggcggtca tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc
    11401 atgttcatca gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc
    11461 agcgcctgtt gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg
    11521 gtcagattcg gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa
    11581 ccgtacagac tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc
    11641 ggttcgttac ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc
    11701 agtaactttg ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc
    11761 cacggtgcgg caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg
    11821 tttttgataa acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa
    11881 gagacaacat aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga
    11941 actgccaccg ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt
    12001 aaatactcgc tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag
    12061 aactcctgcc cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc
    12121 acgccgatta ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac
    12181 cacagcgagc tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg
    12241 taaataatgg cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg
    12301 gctttccagc taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt
    12361 cttgaaaaaa atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc
    12421 agaatttgaa taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta
    12481 tttcagaaaa ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat
    12541 tacgcatacc tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat
    12601 ccggttcctg gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata
    12661 cgttcagcgg gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat
    12721 cggccattag atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca
    12781 ggttgcgtaa ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt
    12841 attccgccag ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg
    12901 agaagaatgg ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca
    12961 ctttttgcag cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc
    13021 aggcaaaaat ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg
    13081 ccggacgtga acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt
    13141 aaggcacaaa atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg
    13201 acagttcatc cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg
    13261 cttccagaat agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat
    13321 atttaaagcg gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg
    13381 gcggttgtga cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc
    13441 gaccgtggaa caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct
    13501 gtgcctgata ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat
    13561 agccaatcat caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca
    13621 gctgggtcat gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa
    13681 ccggcatcgc aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg
    13741 acggcgtttt cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa
    13801 tcacctggca ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca
    13861 gaagcggacg tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc
    13921 agctttcgta gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac
    13981 gcgtgctctc ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc
    14041 gcagggtgtc gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact
    14101 ggtagcaagc gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt
    14161 ttggcagttc ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag
    14221 aacgcaggtt tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg
    14281 ccagcagttc aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt
    14341 tcaggaacaa atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg
    14401 cagtgacgtt cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa
    14461 caaattcgac gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca
    14521 ggtaatttgg tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt
    14581 catctaccgg gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta
    14641 actggcgcag gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga
    14701 gctgttttaa acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt
    14761 cggttgggtg agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg
    14821 tgtcttcgct cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg
    14881 ggttgctggc agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca
    14941 ggttcaggaa ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt
    15001 gtaaggtggt gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca
    15061 acttacggat agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt
    15121 ctcccagcac tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca
    15181 tattacccca gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg
    15241 cgcgaatatg ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga
    15301 cgtcaaatgc tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa
    15361 attcacgacg ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac
    15421 accaaaggtg taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag
    15481 gagtttggca acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc
    15541 agcaaaaatg gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt
    15601 ccagatattc atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg
    15661 ggcataacgt ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc
    15721 cgagcaattt ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga
    15781 tcggcggatg cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca
    15841 atgcatcgtt gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat
    15901 ccatatgcaa ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc
    15961 cgaggttgag cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt
    16021 tatcgcgcaa ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc
    16081 cgcgtgctgg atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat
    16141 gacctttatg tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg
    16201 ggcgcagggc ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag
    16261 cagtcgccag aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat
    16321 caaggataaa cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt
    16381 catgctccgt cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat
    16441 cggtatgccc cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga
    16501 atttgttgcg agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc
    16561 agtccgccag cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc
    16621 ttattgaagg tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt
    16681 tcattgttga cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa
    16741 aaatacacta acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt
    16801 aacctggcaa ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg
    16861 tgccagcggc tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa
    16921 cataaccgct ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt
    16981 gcatccgcag ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga
    17041 gtttagccca ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt
    17101 agcgccgcag tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt
    17161 taacgacgcc accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt
    17221 ggaacaggca gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt
    17281 gattgcggtg ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga
    17341 tgccgatctt cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg
    17401 tgcaggacgt aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg
    17461 cgtctttacc catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt
    17521 caccccgcat ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa
    17581 accgggtgtg acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc
    17641 gctggtgcgg ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg
    17701 ttgcgatatt gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa
    17761 cttactgaaa ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc
    17821 ggaaacgcag tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg
    17881 gcgtactgct ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc
    17941 gtgagtcaca tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc
    18001 tgatgaaagg gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg
    18061 atcagataga cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat
    18121 gggcgaagaa acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca
    18181 aagtgaccca gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta
    18241 agcttatcaa caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa
    18301 cagacgaagg gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa
    18361 cgctgggcgc ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc
    18421 aacgcattgc cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta
    18481 ctgacggcat gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc
    18541 cggtagatat cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc
    18601 cgatgggtac gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt
    18661 gaatttcaaa ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag
    18721 cagatcaacg gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc
    18781 aggatattgt tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg
    18841 cagaagagca ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca
    18901 ggccacaaca aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac
    18961 tgatcgacaa agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc
    19021 aggtagcgac agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta
    19081 accggcagct gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc
    19141 caggttacac tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct
    19201 atgttgagat gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg
    19261 tcagcccatt aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt
    19321 tagcaggctg gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc
    19381 gtgaccatgt gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt
    19441 ttgctgaaga tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc
    19501 gcgtgacttc cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga
    19561 ttcgcggtaa atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag
    19621 gtttgccgct ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc
    19681 tcgatacctg gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga
    19741 aacgtccacg ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg
    19801 attatctggt ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg
    19861 tggtggaagc cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga
    19921 aattcagccc ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg
    19981 acaagcgtgc ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg
    20041 cgcaggcgcg gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg
    20101 ggctttttta tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt
    20161 agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc
    20221 cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc
    20281 tattctacct atcgccatga actatcgtgg caatggagga tggataatga atattcgtga
    20341 tcttgagtac ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg
    20401 ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt
    20461 gatgttgctg gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt
    20521 agatcaggcg cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca
    20581 gggcgagacg atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct
    20641 gctaccgcat attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca
    20701 tgaagcacag acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat
    20761 cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat
    20821 gttgctggct atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga
    20881 tctggcaggg gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat
    20941 gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga
    21001 gacgctgcgc aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt
    21061 gccgccggag cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg
    21121 ccgcactatt ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct
    21181 ggcagaggcc atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt
    21241 ttaaaccgtt taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg
    21301 tgttgacgaa gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga
    21361 tatgaataat ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt
    21421 cccgatggaa caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca
    21481 gatgtttaaa ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt
    21541 cggttttgcc cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa
    21601 tcagatgtgc ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat
    21661 aggccgctga tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct
    21721 gtgcggtctg atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa
    21781 tgttctgtaa cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt
    21841 cagctttcag ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga
    21901 tcttctcgta ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga
    21961 gagaatctga catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat
    22021 ccacttttac atccataccg cggaagatcg acgcatattc acagccgatc actccagcac
    22081 catagataag tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa
    22141 tgcgtggatg ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa
    22201 caaatttttc agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat
    22261 gctcgtcaac aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc
    22321 cctgacgcat gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa
    22381 aagaagagcg gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa
    22441 tgcggctgac ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc
    22501 cgccaacatt ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca
    22561 ttgcagcgcc ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat
    22621 gtggcatggt agggcttacc tgttcttata cataaaagca acagaatggt aacattttat
    22681 cgcgggtaag ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt
    22741 tgtctgtttc ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt
    22801 ttaagcggca ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt
    22861 tttattgcgt taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta
    22921 tagtgccagc agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca
    22981 aaaagaaaaa acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg
    23041 cagcttcacc agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc
    23101 tttttatcgg catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg
    23161 tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt
    23221 gatccgcacc tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg
    23281 gttattattg cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga
    23341 aattcagcac ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg
    23401 tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga
    23461 ggcgttggac gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact
    23521 gcgaatgatt tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat
    23581 tattccggga aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg
    23641 ctgctggcgt tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc
    23701 attgtgccat tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat
    23761 caacgttatc ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt
    23821 attctcggcg tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct
    23881 aacttcttcc cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt
    23941 aaccgtaagc aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca
    24001 tgggttttac ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg
    24061 atcaaacaga gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca
    24121 taacgtttcc gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc
    24181 ggtttcactg tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact
    24241 ctttaagtcg atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa
    24301 ttcttctgcc gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta
    24361 ttgcgcagca gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg
    24421 cgccagcgct aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga
    24481 gcctttggtt acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg
    24541 ggtaaagctg ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc
    24601 gtcgatgtaa tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac
    24661 attcagattc tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc
    24721 atccagcttc ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta
    24781 atcaatctgg aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc
    24841 cgtcatcaac tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct
    24901 tttggtttgt tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa
    24961 ctccgcgcgc atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga
    25021 aaacggtgcc atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg
    25081 ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat
    25141 tgtagggatt gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct
    25201 ggacagtaac ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa
    25261 tccagtgcga atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg
    25321 cggacactgc catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc
    25381 ccgaagacct gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac
    25441 ctgcggcatc cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga
    25501 cggcgtgttc tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg
    25561 tcgttactgc taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg
    25621 tgacccgtca ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc
    25681 ttccgggcgt cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc
    25741 gcggtacaaa tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg
    25801 gggtgagtgg ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat
    25861 atatccgtgg gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata
    25921 tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt
    25981 atcagaacta tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt
    26041 tgggcgatta tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc
    26101 aagcgcagac agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata
    26161 actttactga tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt
    26221 atgacgcgta ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa
    26281 gttgggacgc cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct
    26341 atagccatag caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga
    26401 cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg
    26461 gtagtattgg tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg
    26521 ttgagaatgg atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg
    26581 gcgattttac ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg
    26641 gaacctggca aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct
    26701 acgggacatc ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga
    26761 atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg
    26821 tgaactggcg tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc
    26881 acaccctgaa atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca
    26941 attttgatac cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg
    27001 cgattaccga cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact
    27061 ggcagttgta tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata
    27121 aggattactc atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc
    27181 ttgcggttgc gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt
    27241 tcgacaaaga ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt
    27301 ctggcagcta caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt
    27361 gggttgtcgg tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct
    27421 ttcgataacg tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg
    27481 gtggcaattg tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc
    27541 aacaccgcca gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt
    27601 ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta
    27661 ctggcaaccc gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct
    27721 aatgaatgcc agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag
    27781 cttcatggcg aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga
    27841 atgaaagagc cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa
    27901 gaactcttac aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct
    27961 cgcagaacgg cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat
    28021 attgcctttt gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt
    28081 tacggcttcg aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc
    28141 ggtcagaaaa
//
LOCUS       DAZKGS010000055        26625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKGS010000055 DAZKGS010000000
VERSION     DAZKGS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..482
                     /gene="tnpA"
                     /locus_tag="ACHOQY_002236"
     CDS             51..482
                     /gene="tnpA"
                     /locus_tag="ACHOQY_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9678325.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(498..686)
                     /locus_tag="ACHOQY_002237"
     CDS             complement(498..686)
                     /locus_tag="ACHOQY_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9678326.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(690..1049)
                     /gene="yeaR"
                     /locus_tag="ACHOQY_002238"
     CDS             complement(690..1049)
                     /gene="yeaR"
                     /locus_tag="ACHOQY_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9678327.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1222..1860)
                     /gene="leuE"
                     /locus_tag="ACHOQY_002239"
     CDS             complement(1222..1860)
                     /gene="leuE"
                     /locus_tag="ACHOQY_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9678328.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1987..2910)
                     /gene="dmlR"
                     /locus_tag="ACHOQY_002240"
     CDS             complement(1987..2910)
                     /gene="dmlR"
                     /locus_tag="ACHOQY_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP9678329.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3013..4098
                     /gene="dmlA"
                     /locus_tag="ACHOQY_002241"
     CDS             3013..4098
                     /gene="dmlA"
                     /locus_tag="ACHOQY_002241"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP9678330.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4349..5959
                     /gene="yeaV"
                     /locus_tag="ACHOQY_002242"
     CDS             4349..5959
                     /gene="yeaV"
                     /locus_tag="ACHOQY_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP9678331.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5991..7115
                     /gene="yeaW"
                     /locus_tag="ACHOQY_002243"
     CDS             5991..7115
                     /gene="yeaW"
                     /locus_tag="ACHOQY_002243"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP9678332.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7171..8136
                     /gene="yeaX"
                     /locus_tag="ACHOQY_002244"
     CDS             7171..8136
                     /gene="yeaX"
                     /locus_tag="ACHOQY_002244"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP9678333.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8190..9305)
                     /gene="rnd"
                     /locus_tag="ACHOQY_002245"
     CDS             complement(8190..9305)
                     /gene="rnd"
                     /locus_tag="ACHOQY_002245"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9678334.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9387..11072)
                     /gene="fadD"
                     /locus_tag="ACHOQY_002246"
     CDS             complement(9387..11072)
                     /gene="fadD"
                     /locus_tag="ACHOQY_002246"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9678335.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11277..11858)
                     /gene="yeaY"
                     /locus_tag="ACHOQY_002247"
     CDS             complement(11277..11858)
                     /gene="yeaY"
                     /locus_tag="ACHOQY_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9678336.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11898..12593)
                     /gene="tsaB"
                     /locus_tag="ACHOQY_002248"
     CDS             complement(11898..12593)
                     /gene="tsaB"
                     /locus_tag="ACHOQY_002248"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9678337.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12651..14561)
                     /gene="yoaA"
                     /locus_tag="ACHOQY_002249"
     CDS             complement(12651..14561)
                     /gene="yoaA"
                     /locus_tag="ACHOQY_002249"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9678338.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14693..15037
                     /gene="yoaB"
                     /locus_tag="ACHOQY_002250"
     CDS             14693..15037
                     /gene="yoaB"
                     /locus_tag="ACHOQY_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9678339.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            15460..15759
                     /gene="yoaC"
                     /locus_tag="ACHOQY_002251"
     CDS             15460..15759
                     /gene="yoaC"
                     /locus_tag="ACHOQY_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9678340.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(15879..16058)
                     /gene="yoaH"
                     /locus_tag="ACHOQY_002252"
     CDS             complement(15879..16058)
                     /gene="yoaH"
                     /locus_tag="ACHOQY_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9678341.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16132..17493
                     /gene="pabB"
                     /locus_tag="ACHOQY_002253"
     CDS             16132..17493
                     /gene="pabB"
                     /locus_tag="ACHOQY_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9678342.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17497..18075
                     /gene="nudL"
                     /locus_tag="ACHOQY_002254"
     CDS             17497..18075
                     /gene="nudL"
                     /locus_tag="ACHOQY_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9678343.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18259..19623
                     /gene="sdaA"
                     /locus_tag="ACHOQY_002255"
     CDS             18259..19623
                     /gene="sdaA"
                     /locus_tag="ACHOQY_002255"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9678344.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19754..21352
                     /gene="pdeD"
                     /locus_tag="ACHOQY_002256"
     CDS             19754..21352
                     /gene="pdeD"
                     /locus_tag="ACHOQY_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9678345.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(21356..22912)
                     /gene="yoaE"
                     /locus_tag="ACHOQY_002257"
     CDS             complement(21356..22912)
                     /gene="yoaE"
                     /locus_tag="ACHOQY_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9678346.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(22900..23058)
                     /gene="yoaL"
                     /locus_tag="ACHOQY_002258"
     CDS             complement(22900..23058)
                     /gene="yoaL"
                     /locus_tag="ACHOQY_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9678347.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            23375..24346
                     /gene="manX"
                     /locus_tag="ACHOQY_002259"
     CDS             23375..24346
                     /gene="manX"
                     /locus_tag="ACHOQY_002259"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9678348.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            24409..25209
                     /gene="manY"
                     /locus_tag="ACHOQY_002260"
     CDS             24409..25209
                     /gene="manY"
                     /locus_tag="ACHOQY_002260"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9678349.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25222..26073
                     /gene="manZ"
                     /locus_tag="ACHOQY_002261"
     CDS             25222..26073
                     /gene="manZ"
                     /locus_tag="ACHOQY_002261"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9678350.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26128..26586
                     /gene="yobD"
                     /locus_tag="ACHOQY_002262"
     CDS             26128..26586
                     /gene="yobD"
                     /locus_tag="ACHOQY_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9678351.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6510 a         6879 c         6612 g         6624 t
ORIGIN      
        1 cacctggtct taactggaat ttaaatgcct gtaatcgttt cacaggtcaa atgatacttt
       61 ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca ttgtgttttc ctgatgcatg
      121 ttcacctggt ctttgtcacc agataccgac tccagatttt tgaccatgac gcgacagaaa
      181 aactacgcac ttacttttca aatgtatgtg ctgattttga agctgaactt gttgaaatgg
      241 atggcgaacc agatcacgtc catttgttaa ttaactatcc tcccaaactg gcgatatcca
      301 gtctggtaaa cagcctcaaa ggcgtatcgg gtaggttact gcgacgagat cgaccagata
      361 ttgcagtcag gtattactac aaaggcgttt tgtggagtcc tggctatttt gccagtagct
      421 gcggaggtgc gccaatatcc gtcatccgcc aatacattga acaacagcaa acacctggtt
      481 agatggaaaa ccgcgcctta tatccccgcc ctgaaggacg gggttttacg gcgcaccgga
      541 taagaacgca cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc
      601 aaagtcatcg gcgtctctgt ttcataatcg acagaaacgc cattgctgtt attggtgacg
      661 gtcacggtat acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt
      721 tgcgcacctt ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg
      781 tcagtcatgg cttcaatgtt gtgccacttt tctggaggga acaccgcaaa ctgccccgct
      841 tcgataagga tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg
      901 accgccccat gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga
      961 tgacgttcga atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta
     1021 tgaatataat tctgtgggat ttgcagcatc ctattccctc cttcggggaa tgcgctgaaa
     1081 acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat
     1141 tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt ggggagcccg
     1201 cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac
     1261 gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta
     1321 ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc
     1381 cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa
     1441 ctgtacgaaa aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa
     1501 cgcgcgttta aaaatggccc cgtattgggg ctcatcggat ttggcctcgt tatttttacc
     1561 cttcagggtc gcgtagagaa ttttactccc cagatagagc aaataaaacg caccaagata
     1621 acgtacaatg ttgaataata tcggggtggt cttaattaat gtcgccactc cagcccatgc
     1681 cagaaacatc aatactgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt
     1741 cataccgcta ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac
     1801 aataaaaatg gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac
     1861 attaacctct ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc
     1921 tgtagcggct tagaaaggag aaaatcaggt tttaacccga tatcaacccg ataattgaat
     1981 cattaatcag gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa
     2041 aaattctacc gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca
     2101 gacgttcgct ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt
     2161 ttcgctaaca tcccaccagg agcgcagagc aatcccctgc ccatcgagac accactgatg
     2221 gacaatttct ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc
     2281 tttattgcgt aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg
     2341 caatgcggaa agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga
     2401 cgcgcagaga atgcggtaat tagtcgccag tttgcgggcg attaaattag gggcgatgtc
     2461 atcgccaatg cgaatatcga gatcaacacc ttcattgacc aaatcgacca accggtcttc
     2521 cacatcaaaa cgtaattcca gttgcggata cgccttagcc agcgctgata acgccggagc
     2581 caccacctgt cgtccaaaac caaagctgct gataatacgc agcatcccct gcggcacctg
     2641 acgcacgtca gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca
     2701 ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa
     2761 caccacgttt agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac
     2821 gcccagttct tccgccacgg cggcaaaacc agcccgacga gccaccagca taaagacgcg
     2881 caaatcattc agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg
     2941 ggattaattc cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga
     3001 agtgagaacc caatgatgaa aacgatgcgt attgctgcga tcccgggaga cgggattggc
     3061 aaagaagtcc ttcctgaagg gattcgcgtg ttacaggctg ccgctgagcg ctggggcttc
     3121 gccttgagtt ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa
     3181 atgatgccgg acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc
     3241 gtcggctggc cggacaccgt tcctgaccat atttcgttgt ggggttcgct gctgaaattt
     3301 cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc
     3361 tgcccgctgg cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc
     3421 gaaggcgaat attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc
     3481 attcaggaat cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa
     3541 cttgcgcaaa gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc
     3601 atcagcatgc cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc
     3661 cgctgggaca agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc
     3721 ttcgatgtgg tggtggcgtc caatttattt ggcgatatcc tttccgatct tggcccggcc
     3781 tgcaccggca ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccg
     3841 tcgctcttcg agccagtcca cggttccgcg ccggatatct acgggaaaaa cattgctaac
     3901 cctatcgcca cgatttgggc cggtgcaatg atgctcgatt ttctcggcaa tggcgatgag
     3961 cgtttccagc aagcgcataa cggtattctg gcagcaattg aagaagtgat tgctcacggg
     4021 ccgaaaacac ctgatatgaa aggcaatgcc accacgccac aggttgccga cgcgatttgc
     4081 aaaattattt tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt
     4141 tttattcgtt ctttgcagta aataacctgc gtcatttcac cttttattgt ttccgtttcg
     4201 tgttttatgg ctttccgtat tcttaattgt ttaatttatg taacatgaaa ataatcaatt
     4261 tgttaaacac aacatacaac tcattaccct gcaaataatt acaactggca aaagccagag
     4321 acgggctttt atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc
     4381 gctgataagc atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc
     4441 acaacaatcg gccgacgcgg ccaacgcaat atacactttt gttacgcgta cgttaggttc
     4501 cgccgtacag gtattggttt tgctggcaat gggactggtg atttatttag ccaccagtaa
     4561 atacggcaat attcgtcttg gcgaaggaaa accggaatac agcacgctct cctggctgtt
     4621 tatgtttatt tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc
     4681 ctattattat caaacgcctg gattaaatat cgcaccgcgt tcacaacagg cactcgaatt
     4741 tagcgtccct tactccttct tccactgggg catcagcgcc tgggcaactt atacgctggc
     4801 ctcattaatc atggcttatc actttcatgt gcgtaaaaac aaaggtctga gtctttccgg
     4861 cattattgcc gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt
     4921 gatgttcctg atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac
     4981 ctttactcgt ggactttccg cgctgaccgg tttacccgat aacttcaccg tgcaggcatt
     5041 tgtgatcctg ctttccggcg gcattttttg cctaagctcg tggattggta tcaacaacgg
     5101 tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct
     5161 gattgtcggc ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gtctgaccac
     5221 gcaaaacttc ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg
     5281 caactggacc gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt
     5341 tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg
     5401 cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt
     5461 tatcaatggc gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg aaacagctgt
     5521 acagcaagtt ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt
     5581 gatgattatt ttccttgcct cgcatatgga tgcggtggcc tacaccatgg ctgcgaccag
     5641 tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt
     5701 ggtgatcact ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa
     5761 aaccaccgtc gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg
     5821 atttattcgc tggctgaaac aggattacgc cgacattccg gctcatcaag ttgaacatta
     5881 tctcccgcag acaccggttg aagccctgga aaaaacgcca gtgctccctg cgggaaccgt
     5941 attcaaaggc gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc
     6001 tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca
     6061 ttcctgcccg tttttatacc gatcagaacg cgtttgaaca cgaaaaagag aacgtcttcg
     6121 ccaaaagctg gatttgcgtc gctcacagca ttgaactggc gaatgccaat gattatgtga
     6181 cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg
     6241 cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa
     6301 aaaatgtgat tacctgcccg tatcacgcat gggcattcaa actcgatggc aatctggccc
     6361 atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg
     6421 tgcgtctgga agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg
     6481 tagaagatca gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg
     6541 atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg
     6601 ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac
     6661 aggttgatcg ttactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca
     6721 aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct
     6781 ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg acggtcattt
     6841 atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg
     6901 aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg
     6961 aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc
     7021 aggggcgcat catggccgac agtagcggta gtggcatttc cgaacatggt atcgcccatt
     7081 tccataatct gctggcgcag gtgtttaagg actaatgaca tcggcggcgg tattttccgc
     7141 cgctgggctg atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag
     7201 gtgagtcagg tggaaccgct taccgaacag gtgaaacgct tcacgctggt ggcaaccgat
     7261 ggcaaaccgc tgcctgcgtt caccggagga agtcacatca ttgtgcagat gagcgatggt
     7321 gataaccagt acagcaatgc gtattcacta ctgagttcgc cgcatgacac ctcttgttat
     7381 cagattgccg ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag
     7441 gtaaaagtgg gcgatcggtt aacgatttca acgcctaata acctgtttgc gctaattccc
     7501 tcagccagaa agcatctgtt tatcgcgggc ggtattggta tcaccccttt cctgtcgcac
     7561 atggcagagc tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca
     7621 gaaagttgcg catttcgtga tgagctagtc cagcatccgc aggctgagaa agttcacttg
     7681 catcactcat caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct
     7741 ggcacacacg tttatacctg tggtcccgag gcgctaaatg aagcggtaag aagtgaagct
     7801 gcgcgtctgg acatcgtcgc cgatacgctg cactttgagc aatttgctat cgaagacaaa
     7861 accggcgatg cctttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa
     7921 gagatgacta ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt
     7981 cgtgaagggg tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg
     8041 gatcagtatt ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg
     8101 cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct
     8161 gagcgcgccc agtccggctt cggaagattt tactgcggat attcctgcaa taaattgtgt
     8221 aatgcttccg ccatcagctc accacgccag ccggaaatca gcgccggcaa attgttctgc
     8281 ggtttcagtt tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat
     8341 tcggcgctga tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac
     8401 actttacgat aaccaggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc
     8461 ggcaatgtct gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg
     8521 ctaccggata aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgcgacc
     8581 gaccacaaat gctcttcacg cacgacaaag ttcaccgcca gatcacgctc tcgcgccttg
     8641 cgcagtcgcc agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taactgccag
     8701 gcattggtga tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc
     8761 atcaggcggc attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata
     8821 agcttggcgg tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt
     8881 tcggtcagcg gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg
     8941 gaatactctt ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca
     9001 aggatttgcg tgtcaatcaa gggttgtggt aattcgccaa agacattgag gaacacttcc
     9061 agatcttcac tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc
     9121 agcggtgacc agtcggtgat cccgagagga tcaattagcg ccagatgctc gccatcgaaa
     9181 agttgaatca accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatccagg
     9241 gctatcgccg gaaaggcacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta
     9301 atcatttggt aattcaaatt gttttctctt tagtgggcgt caaaaaaaac gccggattaa
     9361 ccggcgtctg acgactgact taacactcag gctttattgt ccactttgcc gcgcgcttcg
     9421 tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac
     9481 tccaccagct tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac
     9541 tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag
     9601 ccggaaggta cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct
     9661 tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg
     9721 acaatgcgca ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca
     9781 tttttgatga tctcatctgt agcatccgga cgctgccagt aacccagcat cacctgcggt
     9841 cctttgacac aaagctcacc cggttgaccc ggtggtactt cattatcatc atcatccacc
     9901 agtttggctt ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca
     9961 tatgggttaa cgctgaccag cggcgcacac tcggtaaggc catagccttc cagcagatac
    10021 tgtcctgtca gtttcaccca acgctctgcc accacttgct gcactggcat ccctccgcct
    10081 gcggaaagat gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca
    10141 ttgaacaagg tgttaacgcc cgtgatagcg gtaaacggat atttcgctaa ctcttttacc
    10201 aaccctggaa tatcgcgcgg gttagtgata agcaggttct gcccacccag ttcgataaac
    10261 agcaggcagt taatggtcag ggcaaaaatg tgatacagcg gcagcgccgt caccaccagc
    10321 tctttgcccg gatgcaacag cggaccatag gtcgcgttaa cctgttccag gttcgccagc
    10381 atattgcggt gagtcagcat cgcgcctttc gccacaccag tggtgccgcc ggtgtattgc
    10441 agaaaagcta aatcttccgg caccagttcg ggtttgacgt actgcatccg gtagccgtta
    10501 tgcagtgcgc tacgaaatga aatggcatct ggcagatggt atttcggcac caaacgcttg
    10561 atgtatttaa caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata
    10621 cgggtcagaa ttacgtgctg aacggcggtt ttatcaacca ctttttccag tgtgtgagca
    10681 aagttagaca cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca
    10741 cgcggggtat acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac
    10801 agcgccaccg gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc
    10861 agccccaacc cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg
    10921 aaggtcatta cctcccccat attcacaaac gcaggttgat cggcgtagcg cgcgaccgac
    10981 tgctcaaaca tatctaccag agattgataa cggtcagggt tgatctccgt cggaacgtcc
    11041 gcgggataac ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc
    11101 gtcatcgcaa ccccaaatga tatacatgcc gttaacataa tattaactca tcataccagc
    11161 ttgataatta cccaacgaaa aggttgcgaa gcgcgtcact atttattttt atctttaccg
    11221 taagaatgca gaaacagcgg accagccgct gtttcttttt ctttaaaaac aagcaattat
    11281 tcagttacaa ctgtttgtac tctcgcgggg ccgggattat accagcccca tccgccgtag
    11341 ccatagggcc agccacgacc gccataaaac catggatcaa tcggctgagg cggcataatc
    11401 acctgctggg ttaaatgcca acgtttgtaa cccgttactt gcatcaccat aaatttatag
    11461 ggcgtattgc cgattttgcc gtcaaccgca ccagtgattg gcccgactac cgtaacaagt
    11521 tgtccacgga aatccaccgg gtccagaaaa ccgttcacat cggcataaat gcgaccgcga
    11581 gaaggttctc ccagcgtcgg tctggctccg ctgtccagcg gtacggtagc aatttccagg
    11641 cgggttttcc cttgctggtt ttgtaccgca accactttgc caccaaagcg tgcctcctga
    11701 ccaacgtaca gctgcggcgc actcatcacc cgaactaaat cttgttgcgg cgtgggactg
    11761 ctgcctttaa tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct
    11821 gccagtatgc ctttgataac attcttttga accgccatga tgcgactcct ttttttctca
    11881 gggcatactc ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt
    11941 aaataaaccg gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacac
    12001 gcaatcggca gcatatcttc cgccgctggc agtaaaactt ctccgtcgcg caaaaccagc
    12061 ccgctctctt taccgagatc cggccaggct tgccagcccg tccctaccgt tacccattcg
    12121 ccggaaagct gttgcattcg ttcatgaacg agttcgggtt tgagtacggc ttcggtttct
    12181 tcaccgcgcc agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt
    12241 cgcgcgtcaa tggctgccag cacgcgggtt gcgccgtttt tgcgccacgc cccttgcgcc
    12301 atcgtcatta gcgtggagac gccaatcatc ggtagctctg cgccaagtgc cagcccttgc
    12361 gcaatgccaa taccaatgcg cacgccggta aagctaccgg ggccgcgccc gtaaaccaga
    12421 gcgttaatat cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag
    12481 attcgttgag tatgttcacg agggcaaagc tcaaaatgag cgttgacagt accgtcgttc
    12541 cacagggcga cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg catgggtctt
    12601 cgtgcttaga tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag
    12661 gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac gagtgcgtgg cgcgggcggc
    12721 agactggcga gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc acaaatcacc
    12781 agcacgccac gatcgtcggc gtcgcgaatc agtcgcccta ccccctgttt gagagtaatg
    12841 acggcatctg gtagttgcac ttcatcgaac gggtcgccac cgcgcaaacg acaatcttcc
    12901 atgcgcgctt ttaacagtgg atcatccggc gaggtaaacg gcaacttgtc gataatcacc
    12961 aatgacaatg tatcgccacg cacgtccacc ccttcccaga aactactggt tgccacaaga
    13021 agcgcgttac cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc
    13081 aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg ccagatcacg catcatggcg
    13141 tgcgaggtac aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt
    13201 gccgctaact ggcgagcaga acctggctgg ttggtttgcg gcagattgcg cggcacacag
    13261 agtaacgcct ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct
    13321 atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg
    13381 aagatccagc taccaggctt ttgcgccatt aactctttga atttatccgc cacgctgagc
    13441 ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc
    13501 tgattgatct cttttagccg cttcagccgt gtgcgataca acgtagcgcg ctcaaatgcc
    13561 gcatccagca aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg
    13621 gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg
    13681 ttaccgcgat aacctggctc accgagttgc agacgaaaat cctgcgcact ctgggcaaga
    13741 cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt cggtgcggta agcgatggtg
    13801 atgtcttttg ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg
    13861 gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc cgggatcagt
    13921 tcgccaaatc cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc
    13981 accacatcgg cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc
    14041 gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgtgaa
    14101 tcttccgcca cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag
    14161 cgcagcagga tcacatcgct taagatttgt accggcagat cgccccccgc cagcgcctgc
    14221 tgttcgagac gctcgaggca gaggtagttt gagcgccctt tcagcagcgc cacgttgccc
    14281 gtatatttca atgcctttga gactgttggc aaatcgcggc tgtagagctg atcctgcaac
    14341 gcttttgagc cggtcgagat aatgactttc tttttcgccc gcagcgcagg agctaggtaa
    14401 gcgtaggttt tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc
    14461 gcctgggtga cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc
    14521 gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg
    14581 aacagggatt atgtcaggat gagggcgctt tcgccagttg aattggtgac ggcgacctca
    14641 cattgtggca gtctttgcag cacgaaaatg gaaggataat gaggacaaaa tgatgactat
    14701 cgttcgtatc gatgctgaag cccgctggtc tgatgtggta atccacaaca acacgctcta
    14761 ctacactggt gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct
    14821 ggcgcagatt gatgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc
    14881 caccattttc ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg
    14941 ggttgtcgcg ggtcatgcgc cggtgcgctg cacggtacaa gcgggtttga tgaacccgaa
    15001 gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc
    15061 agacgataat ttatcgataa tactggtcgg ttttacataa atcgaccaga gattaagata
    15121 ttacctgttg atgctcctgc taattcatta tttattttta taatttcaat tttatctata
    15181 aaactaacta tataaataac cataaccatg ccgttgtgtt cgccctctga ctagcaactc
    15241 tggttttctt tcttagctga ataatctggt tatagcgtga ggactgaccg ttaaatttct
    15301 ccatcttact ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg
    15361 ttaactcgtg ccatatcgtt ataatccctt tgatattaga tgcaaattaa ggtcatatat
    15421 agtcttatcg ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa
    15481 gccctggatt tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa
    15541 agcacggcga agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct
    15601 gatattacgg cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt
    15661 ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc tgaatacttt
    15721 tcaacatata tgcaggaaga actaaaagca ctggtctgag ttaaatttat atcagcataa
    15781 atgggtcagg acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaatgat
    15841 catcggcttc tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa
    15901 cgcgccacga ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt
    15961 gcctggccgc tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc
    16021 tgctgttcat gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt
    16081 ggcgctaatt atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg
    16141 ttatctcccg ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc
    16201 cgcttaagcc acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat
    16261 agccgctttg atattgtggt cgccgatccg atttgcactt taaccacttt cggtaaagaa
    16321 accgttgtta gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc
    16381 cagcaggtgc tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag
    16441 ggcggcgcac tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa
    16501 attgcggaac aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc
    16561 attgtcgacc accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt
    16621 cgggcctggc tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac
    16681 tggcaatcca atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat
    16741 ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct
    16801 ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct
    16861 tttttacgtc ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt
    16921 gataatagtg aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct
    16981 caggaagata gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat
    17041 ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta
    17101 aaagtaccag agctcttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact
    17161 ataacggcgc gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct
    17221 ggtggctcaa taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa
    17281 ccgcagcgac gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg
    17341 gataccagca ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg
    17401 ggcggtggaa ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa
    17461 gttaataaga tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt
    17521 gatgattttt tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat
    17581 catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg
    17641 actcagcgtt caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca
    17701 gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc
    17761 gctataccgc cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact
    17821 ggctatcagg taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt
    17881 gaagatgaag tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt
    17941 tatcaccctt tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac
    18001 gaacagtatt ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt
    18061 ggtgtgaaac cctgactata cttatcttta catctacaaa acactacttg agacaatcat
    18121 cgcaatatta gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag
    18181 tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac
    18241 cttgtcagga gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc
    18301 tcatcttccc ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc
    18361 gaaaaaggct tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg
    18421 ctgacgggta aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa
    18481 cctgccaccg tggatatcga cagtattccc ggttttattc gcgacgtaga agagcgcgaa
    18541 cgtctgctgc tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt
    18601 tttcataacg gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc
    18661 gatgaagtcg tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa
    18721 gaacactttg gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc
    18781 accgaactgc tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag
    18841 aacgaactgg cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa
    18901 accatgcagg catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg
    18961 cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgttag tttccagcga taaactgtct
    19021 aacgatccga tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa
    19081 aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg
    19141 gcagtgctgg cttactatga ccactttatt gaatcggtca gcccggacat ctatacccgt
    19201 tactttatgg cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt
    19261 gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt
    19321 gcagaactgc tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa
    19381 cacaaccttg gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag
    19441 cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc
    19501 agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac
    19561 atgaacgcca aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac
    19621 taatacttct tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct
    19681 gctgtaatat tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta
    19741 cagggtggcc cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat
    19801 gattgtttgt acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat
    19861 ttcacagcgt aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga
    19921 attagataaa gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg
    19981 tctgccctgc tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt
    20041 gcgatccatt ggcctggtgc aagacggcac actctattgc tccagcattt ttggttatcg
    20101 caatgtgccc gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct
    20161 gacgatcgac cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc
    20221 gggcagtagc aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct
    20281 ccttgagcca caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt
    20341 gctctatggt aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca
    20401 ggtttcttcg caacgctttc cttttaccat taacgttaat ggtccggggg ctacggcgct
    20461 ggcatggcac tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg
    20521 ctacatcgcc tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa tcaatctggg
    20581 cctggcgcaa catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca
    20641 atgtattggt gtagagattt tgctgcgctg gaataatccg cgtcagggct ggatttcacc
    20701 ggatgtgttt attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt
    20761 gatggcagaa accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg
    20821 cattaacgtc gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta
    20881 ctggtttagc gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt
    20941 actggatgtt gattatcgga ttgcccgcga gctacatcgt aaaaacgtca aactggcgat
    21001 tgatgacttc ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt
    21061 gctgaaaatt gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt
    21121 gaccgatatc atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt
    21181 ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta
    21241 tttgtacgca cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc
    21301 gccgcccgcc cggcataatg gacatatcac gcccattatg ccgttacgtt aatgcttact
    21361 catcttcatc gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat
    21421 tggcttcaat gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa
    21481 tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa
    21541 ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc
    21601 agccgtcacc atcagtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa
    21661 tggcttccag cacatccagc ggcgtgacca gaccttgtac cacaccaaac tcgttggtca
    21721 cgataacaaa gctcccgcga gcacgacgca gcacgcccaa caggttgatc ggatcgaggg
    21781 tttccgggac gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt
    21841 ccagcgccac cagcagttct ttagcacgta caataccgat gatttcatcc agttcaccgc
    21901 gacataccgg gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc
    21961 cgagattagc gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac
    22021 gcgacgccag cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca aatgcacctt
    22081 ccgggatcgg catcggcatc gggttatcgg catcgtgctg aacattggcc tgacgtttcc
    22141 cgcccatcaa acgcaggatg gcatcggcag tacgcgctcg cagcggcaaa gtcgactggt
    22201 ggcgaataaa gttgcgacgc gcaatctggt taaacacttc gatgatgatc gagaagccaa
    22261 tcgcggcata caggtaacct ttcggaatgt ggaaaccgaa accttctgcc accagactca
    22321 gaccaatcat taacaggaag ctcagacaga gcaccaccac cgtggggtgc tggttaacga
    22381 atcgcgtcag cggtttggat gccagcaaca taaccgccat cgcaatcact accgccgcca
    22441 tcatcaccgg cagatggtta accatcccta ctgcagtaat taccgcatcc aacgagaaga
    22501 cggcgtcaag gatgacaatc tgtgtgacga ccacccagaa actggcgtag cctttaccgt
    22561 ggccggaatc atgatcgcgg ttttccagcc gttcatgcag ttcggttgtt gctttgaaca
    22621 gcaagaatat ccccccgaac aacataatca ggtcgcgtcc ggagaaggag aaatccatga
    22681 cggtaaatag cggtttggtc agcgtgacca tccatgaaat cagcgacagc agccccagac
    22741 gcataatcag cgccagtgat aaccccagca aacgcgcttt atcgcgttgt tttggcggca
    22801 gtttgtcagc aagaatggcg atgaagacca ggttatcgat acccagcaca atttcgagaa
    22861 caacaagcgt gagtagcccc gcccaaattg aggggtccat taagaattcc atgacaagct
    22921 cctgcttaag gaatagctat tcgacgccag aaataatgca ggcgtaacga caaaatgcaa
    22981 acgaaaggtg cggcatagag agccagaaag gtaggcgtta aaaggcctga tgctgaaatg
    23041 acgtcggtga cgatccatac tgcgggctac tgccctatac tccatggttg ttaaacggga
    23101 gttaaacata tcagagacgc ctctgatttg gcaaagattt accttccttt gcaaacgaat
    23161 gtgacaagga tattttacct ttcgaaattt ctgctaatcg aaagttaaat tacggatctt
    23221 catcacataa aataattttt tcgatatcta aaataaatcg cgaaacgcag gggtttttgg
    23281 ttgtagccct tatctgaatc gattcgattg tggacgacga ttcaaaaata catctggcac
    23341 gttgaggtgt taacgataat aaaggaggta gcaagtgacc attgctattg ttataggcac
    23401 acatggttgg gctgcagagc agttgcttaa aacggcagaa atgctgttag gcgagcagga
    23461 aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc gaaacgctga ttgaaaagta
    23521 caacgctcag ttggcaaaac tcgacaccac taaaggcgtg ctgtttctcg ttgatacatg
    23581 gggaggcagc ccgttcaatg ctgccagccg cattgtcgtc gacaaagagc attatgaagt
    23641 cattgcaggc gttaacattc caatgctcgt ggaaacgtta atggcccgtg atgatgaccc
    23701 aagctttgat gaactggtgg cactggcagt agaaacaggc cgtgaaggcg tgaaagctct
    23761 gaaagccaaa ccggttgaaa aagccgcgca agcacccgct gccgcagcac caaaagcggc
    23821 tccaactccg gcaaaaccaa tggggccaaa cgactacatg gttattggcc ttgcgcgtat
    23881 cgacgaccgt ctgattcacg gtcaggtcgc cacccgctgg accaaagaaa ccaatgtctc
    23941 ccgtattatt gttgttagtg atgaagtggc tgcggatacc gttcgtaaga cactgctcac
    24001 ccaggttgca cctccgggcg taacagcaca cgtagttgat gttgccaaaa tgattcgcgt
    24061 ctacaacaac ccgaaatatg ctggcgaacg cgtaatgctg ttatttacca acccaacaga
    24121 tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct gttaacgtcg gtggtatggc
    24181 attccgtcag ggtaaaaccc aggtgaataa cgcggtttcg gttgatgaaa aagatatcga
    24241 ggcgttcaag aaactgaatg cgcgcggtat tgagctggaa gtccgtaagg tttccaccga
    24301 tccgaaactg aaaatgatgg atctgatcag caaaatcgat aagtaacgta ttgtgttgat
    24361 tatcactcag ttttcacact taagtcttac gtaaacagga gaagtacaat ggagattacc
    24421 actcttcaaa ttgtgctggt atttatcgta gcctgtatcg caggtatggg atcaatcctc
    24481 gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc tggtgggtat cgttcttggg
    24541 gatatgaaaa ccggtattat tatcggtggt acgctggaaa tgatcgcgct gggctggatg
    24601 aacatcggtg ctgcagttgc gcctgacgcc gctctggctt ctatcatttc taccattctg
    24661 gttatcgcag gtcatcagag cattggtgca ggtatcgcac tggcaatccc tctggccgct
    24721 gcgggccagg tactgaccat catcgttcgt actattaccg ttgctttcca gcacgctgcg
    24781 gataaggctg ctgataacgg caacctgaca gcgatttcct ggatccacgt ttcttctctg
    24841 ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg ttgcgctgtc tgttggtacc
    24901 agcgaagtac agaacatgct gaatgcgatt ccggaagtgg tgaccaatgg tctgaatatc
    24961 gccggtggca tgatcgtggt ggttggttat gcgatggtta tcaacatgat gcgtgctggc
    25021 tacctgatgc cgttcttcta cctcggcttc gtaaccgcag cattcaccaa ctttaacctg
    25081 gttgctctgg gtgtgattgg tactgttatg gcagtgctct acatccaact tagcccgaaa
    25141 tacaaccgcg tagccggtgc gcctgctcag gcagctggta acaacgatct cgataacgaa
    25201 ctggactaac aggtgagcga aatggttgat acaactcaaa ctaccaccga gaaaaaactc
    25261 actcaaagtg atattcgtgg cgtcttcctg cgttctaacc tcttccaggg ttcatggaac
    25321 ttcgaacgta tgcaggcact gggtttctgc ttctctatgg taccggcaat tcgtcgcctc
    25381 taccctgaga acaacgaagc tcgtaaacaa gctattcgcc gtcacctgga gttctttaac
    25441 acccagccgt tcgtggctgc gccgattctc ggcgtaaccc tggcgctgga agaacagcgt
    25501 gctaatggcg cagagatcga cgacggtgct atcaacggta tcaaagtcgg tttgatgggg
    25561 ccgctggctg gtgtaggcga cccgatcttc tggggaaccg tacgtccggt atttgcagca
    25621 ctgggtgccg gtatcgcgat gagcggcagc ctgttaggtc cgctgctgtt cttcatcctg
    25681 tttaacctgg tgcgtctggc aacccgttac tacggcgtag cgtatggtta ctccaaaggt
    25741 atcgatatcg ttaaagatat gggtggtggc ttcctgcaaa aactgacgga aggggcgtct
    25801 atcctcggcc tgtttgtcat gggggcattg gttaacaagt ggacacatgt caacatcccg
    25861 ctggttgtct ctcgcattac tgaccagacg ggcaaagaac acgttactac tgtccagact
    25921 attctggacc agttaatgcc aggcctggta ccactgctgc tgacctttgc ttgtatgtgg
    25981 ctactgcgca aaaaagttaa cccgctgtgg atcatcgttg gcttcttcgt catcggtatc
    26041 gctggttacg cttgcggcct gctgggactg taagactgtt gtacactacc ggggcctttt
    26101 ggccccgttt ttttatctgg aggattaatg acaatcacgg acctggtact gattcttttc
    26161 atcgccgcac tcctggcctt cgcgatctac gatcagttca tcatgccccg ccgtaacggc
    26221 cccaccctgc tggcaattcc tttgctccgg cgtggtcgca tcgatagcgt tatcttcgtc
    26281 ggattgattg tcattcttat ctataacaac gtcacgaatc atggtgcgtt aataacgaca
    26341 tggttattaa gcgcactggc tctgatgggt ttttatatat tctggatccg cgttccgaag
    26401 atcatcttta aacaaaaagg ttttttcttc gccaatgtct ggattgaata tagccgaatc
    26461 aaagcgatga acttgtcgga agatggcgtg ttggtgatgc aattagaaca gcgtcggctg
    26521 ttaatccgcg ttcgaaatat cgacgatctg gaaaaaattt ataagcttct cgtttcaact
    26581 caataagtta tgaatttagc caaagctatg tttagtgtat tttta
//
LOCUS       DAZKGS010000056        26474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKGS010000056 DAZKGS010000000
VERSION     DAZKGS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..400)
                     /gene="yiiR"
                     /locus_tag="ACHOQY_002263"
     CDS             complement(<1..400)
                     /gene="yiiR"
                     /locus_tag="ACHOQY_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9678352.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDT"
     gene            501..1100
                     /gene="yiiQ"
                     /locus_tag="ACHOQY_002264"
     CDS             501..1100
                     /gene="yiiQ"
                     /locus_tag="ACHOQY_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP9678353.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            1208..1975
                     /gene="tpiA"
                     /locus_tag="ACHOQY_002265"
     CDS             1208..1975
                     /gene="tpiA"
                     /locus_tag="ACHOQY_002265"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9678354.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2030..2785)
                     /gene="cdh"
                     /locus_tag="ACHOQY_002266"
     CDS             complement(2030..2785)
                     /gene="cdh"
                     /locus_tag="ACHOQY_002266"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9678355.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(2892..3881)
                     /gene="sbp"
                     /locus_tag="ACHOQY_002267"
     CDS             complement(2892..3881)
                     /gene="sbp"
                     /locus_tag="ACHOQY_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9678356.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(4200..5162)
                     /gene="pfkA"
                     /locus_tag="ACHOQY_002268"
     CDS             complement(4200..5162)
                     /gene="pfkA"
                     /locus_tag="ACHOQY_002268"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9678357.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5343..6245)
                     /gene="fieF"
                     /locus_tag="ACHOQY_002269"
     CDS             complement(5343..6245)
                     /gene="fieF"
                     /locus_tag="ACHOQY_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9678358.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6394..6894)
                     /gene="cpxP"
                     /locus_tag="ACHOQY_002270"
     CDS             complement(6394..6894)
                     /gene="cpxP"
                     /locus_tag="ACHOQY_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9678359.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7044..7742
                     /gene="cpxR"
                     /locus_tag="ACHOQY_002271"
     CDS             7044..7742
                     /gene="cpxR"
                     /locus_tag="ACHOQY_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9678360.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7739..9112
                     /gene="cpxA"
                     /locus_tag="ACHOQY_002272"
     CDS             7739..9112
                     /gene="cpxA"
                     /locus_tag="ACHOQY_002272"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9678361.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9254..9928)
                     /gene="yiiM"
                     /locus_tag="ACHOQY_002273"
     CDS             complement(9254..9928)
                     /gene="yiiM"
                     /locus_tag="ACHOQY_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9678362.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10077..11060)
                     /gene="kdgT"
                     /locus_tag="ACHOQY_002274"
     CDS             complement(10077..11060)
                     /gene="kdgT"
                     /locus_tag="ACHOQY_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9678363.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11320..11940)
                     /gene="sodA"
                     /locus_tag="ACHOQY_002275"
     CDS             complement(11320..11940)
                     /gene="sodA"
                     /locus_tag="ACHOQY_002275"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9678364.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12225..13259
                     /gene="rhaT"
                     /locus_tag="ACHOQY_002276"
     CDS             12225..13259
                     /gene="rhaT"
                     /locus_tag="ACHOQY_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP9678365.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13256..14104)
                     /gene="rhaR"
                     /locus_tag="ACHOQY_002277"
     CDS             complement(13256..14104)
                     /gene="rhaR"
                     /locus_tag="ACHOQY_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP9678366.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14178..15015)
                     /gene="rhaS"
                     /locus_tag="ACHOQY_002278"
                     /pseudo
     CDS             complement(14178..15015)
                     /gene="rhaS"
                     /locus_tag="ACHOQY_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            15303..16772
                     /gene="rhaB"
                     /locus_tag="ACHOQY_002279"
     CDS             15303..16772
                     /gene="rhaB"
                     /locus_tag="ACHOQY_002279"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9678367.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            16769..18028
                     /gene="rhaA"
                     /locus_tag="ACHOQY_002280"
     CDS             16769..18028
                     /gene="rhaA"
                     /locus_tag="ACHOQY_002280"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9678368.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            18111..18935
                     /gene="rhaD"
                     /locus_tag="ACHOQY_002281"
     CDS             18111..18935
                     /gene="rhaD"
                     /locus_tag="ACHOQY_002281"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9678369.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            19007..20155
                     /gene="fucO"
                     /locus_tag="ACHOQY_002282"
     CDS             19007..20155
                     /gene="fucO"
                     /locus_tag="ACHOQY_002282"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9678370.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            20152..20466
                     /gene="rhaM"
                     /locus_tag="ACHOQY_002283"
     CDS             20152..20466
                     /gene="rhaM"
                     /locus_tag="ACHOQY_002283"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9678371.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            20779..21225
                     /gene="frvA"
                     /locus_tag="ACHOQY_002284"
     CDS             20779..21225
                     /gene="frvA"
                     /locus_tag="ACHOQY_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP9678372.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            21236..22687
                     /gene="frvB"
                     /locus_tag="ACHOQY_002285"
     CDS             21236..22687
                     /gene="frvB"
                     /locus_tag="ACHOQY_002285"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP9678373.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            22677..23747
                     /gene="frvX"
                     /locus_tag="ACHOQY_002286"
     CDS             22677..23747
                     /gene="frvX"
                     /locus_tag="ACHOQY_002286"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9678374.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            23747..25495
                     /gene="frvR"
                     /locus_tag="ACHOQY_002287"
     CDS             23747..25495
                     /gene="frvR"
                     /locus_tag="ACHOQY_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP9678375.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISDSGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(25545..>26474)
                     /locus_tag="ACHOQY_002288"
     CDS             complement(25545..>26474)
                     /locus_tag="ACHOQY_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP9678376.1"
                     /translation="QPAAPAPEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYN
                     KLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPA
                     LQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEP
                     VAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVD
                     AAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKE
                     QLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         6456 a         6882 c         6707 g         6429 t
ORIGIN      
        1 gagtatcttt accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa
       61 gcatcatcac cagaatcaac gtcgggacaa aacgccccac cgcccattgc cagacacccg
      121 gtaaaatcgc ccagttaccc gccagcagca tccacgccac aatcatcaga aatgcccatg
      181 cgccggaacg cccgcgatca tgcaagcgct taacagttac tgccgctgtt ggccaaagca
      241 agcacacaag gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc gccagtgaga
      301 aaagcaccag catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa
      361 tacgcccttt aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt
      421 cttttccagg attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt
      481 cataacaaaa ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg
      541 cattaaccgt tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt
      601 atctgctggc tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta
      661 acagccagaa tcctagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca
      721 aagccaatct cactcgtgct gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc
      781 tggagcgcgg taccttaaaa atcaaaagca ttcaaatgac ctggctaccc atccaggggc
      841 cagagcaaaa agcagcgaaa gcgaaagctc aggaatacat ggcagcagtg atccgcacac
      901 tcaccccatt aatgaccaaa acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg
      961 ggaaaaacaa acgttattac accgagacag aaggtgcact gcgttatgtt gtcgcggaca
     1021 acggcgaaaa ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc
     1081 ttgaaggttt gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt
     1141 ttcacagacc tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag
     1201 aattaaaatg cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat
     1261 ggttcacgag ctggtttcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt
     1321 tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag cgcgaagctg aaggcagcca
     1381 catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac
     1441 ttctgccgct atgctgaaag acatcggcgc tcagtacatc atcatcggcc actctgaacg
     1501 tcgtacttac cacaaagagt ctgacgaact gattgcgaaa aaattcgcgg tgctgaaaga
     1561 gcagggcctg actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa
     1621 aactgaagaa gtttgcgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc
     1681 attcgaaggt gcggttatcg cttacgaacc tgtatgggca atcggtactg gcaaatctgc
     1741 aactccggct caggcacagg ctgttcacaa attcatccgt gaccacatcg ctaaagttga
     1801 cgctaacatc gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc
     1861 tgcagaactg tttgctcagc cagatatcga cggcgcgctg gttggcggtg cttctctgaa
     1921 agctgacgct ttcgcagtga tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg
     1981 acaggtgccg gatttcatat ccggcactta ctttccttaa ctcctcgcct taacgcaaaa
     2041 tctcacactc atgatcctgg atttcctcgg ctgaagcacg gttcagcgtc agtagatttc
     2101 gttgtgtcgc cagcaacaca aacgaattat cactctgccg taccatcgcc agcccgtagc
     2161 ttcccatatg ttcccgcgcc tcaggcactt cttctgcaag catcataaat gggctgcgtt
     2221 gtaccagttc gctttccgtt acccgacgcg ccaggtattc atgcccgcgc aaaccacctg
     2281 gcagtggcaa ccagcggctg ctgatgttcg ccagattttt atccagttgt tcgcgcacat
     2341 caggacgaat acaagagata tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt
     2401 tgatcgccaa agaaaccgcg cgatcgggaa ccggctggcc gtattttttg ctcataaaat
     2461 cacgcgcctg ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac
     2521 tttcagtacc gttaatacga tacgttggca tcaacagata ttgcagtgga ccattaagat
     2581 cttttaaaac cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat
     2641 tttgctgctg attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt
     2701 caccggttaa tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca
     2761 aaaaaagaag acccgctttt ttcatctttt tttccctgta cctcaaagag atgcaagggt
     2821 aacgcaaaat cgtgacaaat aaaaaacccg gtcgcgagaa accaccgggt ttgataatta
     2881 tcctggggaa atcagcgttt gctgatctga tcgaacgtac cgccgttagc aaaatgctct
     2941 ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg tgaataactt cagctttgga
     3001 aacgcatttt cgtacttttt cgctacttca gcgtcgcgcg gacggtagta gtttttcgcg
     3061 gcaatttcct gaccttctgg cgagtagaga tatttcaggt aggcttccgc cacctctttg
     3121 gtgccttttt tctcgaccac tttatcgacc accgacacgg ttggctctgc gaggatagac
     3181 tcactcggcg tgacgatttc gaatttatct ttccccagtt cattcgctac cagcagagct
     3241 tcgttttccc aggcgatcag tacatcgcca atcccgcgct cgacaaaggt gttagtggag
     3301 ccgcgcgcgc cagaatccag aacttcgacg tttttataca gtgcccgaac aaaatcctgt
     3361 gcttttgcct gatcgttgtt gttgtgatgc agcgcgtagc cccaggcagc caggtagttc
     3421 cagcgtgcgc caccagagct tttcggatta ggcgtgatca ccgaaacacc cggtttaatc
     3481 agatcgttcc agtcatggat ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg
     3541 gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc actctttatc aatccgcccg
     3601 cgttccgcaa tcgcgtccac gtcataggcc agagccagcg tgacaacatc agcttcaata
     3661 ccgttgatta ccgacgtcgc ttgtttacct gaaccaccgt gtgactgacg aatcaccacg
     3721 ttatcaccag tttgctgttt ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat
     3781 tcgcgcgttg gatcatatga aacgttaaga agctgaatat cctttgccat aacgttggtt
     3841 gccgccagca aaaatgttaa ccctacgccc cacttgttca tcgcccgact ctcttatgtt
     3901 gtgttgtgat gagcaaagcg tgccagaagg ttaaccaaac attaaagaat aaaaaaagat
     3961 tggctataac ttgcgggtat atgttggggg ttaaaaaggc ggaagaaatc cgcctcatat
     4021 tgctgacaaa gtgcgctttg tccatgccgg atgcggcgcg aacgccttat ccggcctaca
     4081 aaagtttgca aattcaataa attgcagaat tcatgtaggc ctgataagcg aagcgcatca
     4141 ggcatttttg cttctgtcat cggtttcagg gtaaaggaat ctgccttttt ccgaaatcat
     4201 taatacagtt ttttcgcgca gtccagccag tcacctttga acggacgctt catgttttcg
     4261 atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac acaacgaccg
     4321 ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc cagaatacgg
     4381 tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc gcgggtttca
     4441 cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat atgttcggta
     4501 atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc gtttaccagg
     4561 tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc ggcaatggcc
     4621 gcagccaacg tcagatcgcc acaataacgg cccatcactt ccaccacgga aatacgctgg
     4681 tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct cagcgcagtg
     4741 aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc cggcagaccg
     4801 atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc gtcaccgccg
     4861 ataaccacca gcgcgtcgat cccacgtttt ttcaggtttt cgatagccac ggcgcggatg
     4921 ttctcgtcgc ggaattccgg gaagcgcgca gaaccgagga acgtaccgcc acggttgatc
     4981 atgtcagaaa cgctgtaacg gtctagctgt accatacggt cttcatacag acccagatag
     5041 ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac aaccccgcga
     5101 attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat tttcttaatc
     5161 atgactacct ctgaactttg gaatgcaaaa taaaatctgt tgccggaagt cttcttgcac
     5221 atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct gaattgattc
     5281 aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct cttttatact
     5341 gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg gtcctgatgg
     5401 ataattacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg atccgccacc
     5461 atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg aataaagcgg
     5521 gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg ccaggaagtc
     5581 acgatatcaa taatttcttg ccgttcctca tcaggcaatg cgcgatccag taatgactgt
     5641 accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat tcccaatgca
     5701 aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag cagaattgcg
     5761 ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc ctggctttgc
     5821 gtccggcgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat tagcgccaca
     5881 attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat cagatgttga
     5941 atacccgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg cgccagcgcc
     6001 gcgagggact ctgctttacc gtgaccaaac gagtgattat cgtcggcagg ttgcagggaa
     6061 tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga atccaccagc
     6121 gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat cagcaatagc
     6181 aacgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc ataagattga
     6241 ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc atctgcaatg
     6301 cattaagcag caggcaaatt gaggataaaa aaaaccccca catcatgtgg gggaagacag
     6361 ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact
     6421 caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg
     6481 ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg
     6541 gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc
     6601 ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc
     6661 cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat
     6721 ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg
     6781 ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct
     6841 taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg
     6901 ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca
     6961 tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt
     7021 tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc
     7081 tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg
     7141 atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa
     7201 tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc
     7261 ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg
     7321 gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg
     7381 cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa caacgacaac ggttcaccga
     7441 cactggaagt tgatgcctta gtgctgaatc caggccgtca ggaagccagc ttcgacgggc
     7501 aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg
     7561 gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt gggcaaacgc ctgacgcctt
     7621 tcgaccgcgc tattgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag
     7681 atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta tctgatggtt tctgcttcat
     7741 gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg cgctggtgtt
     7801 gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag
     7861 cgaacagcgt caggggctga tgattgagca gcatgtcgaa gcggagctgg cgaacgatcc
     7921 gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc
     7981 aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga
     8041 aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa
     8101 aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta
     8161 ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta acttactgtt
     8221 tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg
     8281 gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc
     8341 ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc
     8401 aggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg
     8461 tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac
     8521 ggcgttactg cgccgtcgta gcggtgaaag caaggaactc gagcgtattg aaaccgaagc
     8581 gcaacgtctg gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcaaaaaaa
     8641 cgcgctggtt agcgaaacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc
     8701 ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg
     8761 gccgctgtac ggcaatccga acgccctgga aagtgcgctg gaaaacattg ttcgtaatgc
     8821 tctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac
     8881 cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg
     8941 tccgttctat cggaccgatg aagcacgcga tcgtgaatct ggcggtacag gattggggct
     9001 ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc
     9061 gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca
     9121 tttgtaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat aaggcgttta
     9181 cgccgcatcc ggcatttgag caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc
     9241 tacactccgg gggttatttt ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca
     9301 gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa cccggcggcg gagagtaaac
     9361 ggtgatactg atcgtcatca aacggcatat gccatgcgat ggcggcagcc tcctgcacgg
     9421 tcacatcgct gacacgggaa accaactcca gcggcgcgtc cgcagacact ttccctggcg
     9481 ctatcacgct atacaaccac cccaccttac ctgtgttttg catcaactgc gctatatcgc
     9541 tgatatcaaa atggtaattg agtttatagc aaggcgagcg cggctggctg acctgaatta
     9601 atgcctctcc ccagcggaaa atatcgccca tatgaacatt actttccgtg agaccgtcgg
     9661 ttgagagatt ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc
     9721 gcacccagta gagatagtgc tcacgaggat aatgacacag cgctctgtct ggcccgccgt
     9781 gaaccttttt ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc atcaactcac
     9841 catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc tgaatcttgc
     9901 ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat ttccccatta
     9961 gaccatattt attttatgtc aggcgtgctg ttttcgcggt aagtatccag gcaggaactc
    10021 aatagcaaac cggacgaacg ttagttcatc cggctgtggg gattaaggaa gcaggtttat
    10081 ttcaccgtac ctaaaatttc gattttcgct gctctggctt tgactttacg tgaccagata
    10141 gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac cagcgaagtt
    10201 gctgccggag ccatcggttt aaacgcaggc accatttctg caatcagcac aggtgtcgct
    10261 accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc accgccaatc
    10321 aatttatcgg cgataatcaa cggaataccg gtcacgataa ttactgccac acccaacagg
    10381 atccccagca aaccagtctg ggcaatcaca gtcaaatcaa tggtattgcc cagcgcgaag
    10441 gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa attctcgtaa ttcagggtca
    10501 aggttcccca gggcaaagcc caccaggaac ggtaatactg cgccgacgaa aacatgcggt
    10561 tcaaacgagg caatccctgc agtgcccaga ataatcatcg tcatgagcgg cccggactcc
    10621 aacgacatca acacaaatgc cccagcttct tcttttgtgc cgtactgctg catgatggaa
    10681 gcgtaaagtc cgccattggt catatccatc gccgccacca gcgccagcgt tgaaagtccg
    10741 gcaaagaatc caacttcaac accatgttcc ggaataatgc gcgaggcaat tgccgcaacc
    10801 acccacgcga cggcaatttt tgttaccacc agcgtaccgg atttacgcaa taccgttccc
    10861 gtcgcgctta attttattga cgcccccatg caaaaaaacc acaccgccag aatgggcacc
    10921 gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc cggcgagaag
    10981 gtgtggcaca gtgcgccaag gaatagcggg acgagcatca tcccccccgg gattttctca
    11041 atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg ctgatgcgtc
    11101 attcctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt taatcaataa
    11161 aagtgatcaa aaccacatca attgaaacgc tgttttattt ttatcggatc attgttttta
    11221 ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt ttaagctgat
    11281 atgcggccta tacgcctcat tgcagcaggc ggcaaatgat tattttttcg ccgcaaaacg
    11341 tgccgctgct tcgtcccagt tcaccacgtt ccagaactct ttaatgtagt ccggacggcg
    11401 gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa tcgggaagcc
    11461 ggaagcgcca gaaatagctt cacccatcag cggagaatcc tggttagcag tagaaaccac
    11521 cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc gggaagctgc
    11581 cgctttttca aattctgctt tgaagttatc aacggagccg aagtcacgtt cgatagccgc
    11641 tttcaggtca ccctgcaggg tggtgccttt tttcagacct ttccagaaca ggctgtggtt
    11701 agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca gctggtccag
    11761 tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc
    11821 gttggcgttg tttacgtagg tctgatggtg tttggtgtgg tggatttcca tggtctgctt
    11881 atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg tatagctcat
    11941 attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt
    12001 atagttaatt aaatgatatt gaaaatgatt atcaatgccg tacttttcgt aagggtatgg
    12061 ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg tgattgccag
    12121 taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt ttacactcat
    12181 ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa gaagatgagt aacgcgatta
    12241 cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt tacgctccgt
    12301 tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt
    12361 ggattattct gccgtgggcc atcagcgccc tgttactacc ggatttctgg gcgtattaca
    12421 gctcgtttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg tgggggatcg
    12481 gtaatatcaa ctacggcctg accatgcgtt atctcggcat gtcgatggga attggcatcg
    12541 ccattggcat tacgttgatt gtcggtacac tgatgacgcc aattatcaac ggcaatttcg
    12601 atgtgctgat taacaccgaa ggcggacgca tgacgttgct cggcgttctg gtggcgctga
    12661 ttggcgtagg gattgtaact cgagccgggc agttgaaaga gcgcaagatg ggcattaaag
    12721 ccgaagagtt caatctgaaa aaagggctgg tgctggcggt tatgtgcggc attttctctg
    12781 ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct gccgcacttg
    12841 gcgtcgatcc actgtatgtc gctctgccaa gctatgttgt catcatgggc ggcggcgcga
    12901 tcattaacct cggtttctgt tttattcgtc tggcaaaagt gaaggatttg tcgctaaaag
    12961 ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt gttactctcg gcactgggcg
    13021 ggttgatgtg gtatctgcaa ttctttttct atgcctgggg ccacgcccgc attccggcgc
    13081 agtatgacta catcagttgg atgctgcata tgagtttcta tgtattgtgc ggcggtatcg
    13141 tcggactggt gctgaaagag tggagcaatg caggacgccg tccggtaacg gtgttgagcc
    13201 tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg catcggcatg gcgaattaat
    13261 ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca
    13321 ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca
    13381 ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgtcgc agatattgat
    13441 tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg
    13501 cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc agccgggtaa
    13561 tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat
    13621 ggcgattcag caacatcgcc aactgcccga acagcaactc tgccatttcg ttagcaaacg
    13681 gcacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc tgcgccatcc
    13741 ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc ggaatcgccc
    13801 cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc tgcaaaacca
    13861 gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag agatcgccac
    13921 gggtaatgcg ataagggcga tcgttgagta catgcaggcc attaccgcgc cagacaatca
    13981 ccagctcaca aaaatcatgt gtatgttcag caaagacatg ttgcggataa cggtcagcca
    14041 cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg
    14101 ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata
    14161 cgttgagaag attcgcgtta ttgcagaaaa ccatcccgtc cctgacgaat atcacgcggt
    14221 gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcaa
    14281 tgataggcga tgtcagtaac gctggcctca ctgtggcgta gcagatgtcg ggccttcatc
    14341 agtcgcaggc ggttcaggta acgctgaggc gtcagtcccg tttgctgctt aagctgccga
    14401 tgtagcgttc gcagtgaaag agaaaattga tccgccacgg catcccaatt cacctcatcg
    14461 gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct gttttccagg
    14521 ttcttctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa agatctcgcg
    14581 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
    14641 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
    14701 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    14761 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    14821 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    14881 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    14941 ctgagggagc cggggttcta tcgccacgga cgcgttacca gacggaaaaa aatccacact
    15001 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    15061 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
    15121 atacagcgtg aattttcagg aaatgcggtg agcatcacat caccacaatg aagcaaattg
    15181 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
    15241 agaaggtcac gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca
    15301 ttatgacctt tcgcaattgt gtcgccgtca atctcggcgc atccagtggg cgcgtgatgc
    15361 tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaaa
    15421 atgggctgca tagccagaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca
    15481 ttcgccttgg cttaaacaaa gtgtgtgagg aagggattcg tatcgatagc attgggattg
    15541 atacctgggg cgtggacttt gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg
    15601 ttgcttatcg cgatagccgc accaatggcc taatggcgca ggcacagcaa caactcggca
    15661 aacgcgatat ttatcaacgt agcggcatcc agtttctgcc cttcaatacg ctttatcagt
    15721 tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga
    15781 tgccggatta cttcagttat cgcctgaccg gcaagatgaa ctgggagtac accaatgcca
    15841 ccaccacaca actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga
    15901 gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc attgggcact
    15961 ggatttgccc gcagggtaat gagattccgg tggtcgccgt tgccagccat gataccgcca
    16021 gcgcggttat cgcctcgccg ttaaacggtt cacgcgccgc ttatctctct tctggcacct
    16081 ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca
    16141 acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct
    16201 tatggctgct tcagcgagtg cttcaggaac ggcaaatcaa cgatctcccg gcgcttatcg
    16261 ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa tcccaatgac gatcgcttta
    16321 ttaatcctga ggcgatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga
    16381 tcccggaaag tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg
    16441 ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga tttctcgcaa ctgcatattg
    16501 tcggcggcgg ctgccagaac acgctgctca accagctatg cgccgatgcc tgcggtattc
    16561 gggtgatcgc cgggcctgtt gaagcctcaa cgctcggcaa tatcggcatc cagttaatga
    16621 cgctggatga actcaacaat gtggataatt tccgtcaggt cgtcagcacc accgcgaatc
    16681 tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact
    16741 ctacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct
    16801 ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag gaggcgctgc gccaacttga
    16861 tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc
    16921 ggaaggttcg ctgaccggtg ggattcaagc tactggtaat tatccgggca aagcgcgtaa
    16981 tgccagtgag ctacgtaccg atctggaaca ggctatgcgg ctgattccgg ggccgaaacg
    17041 gcttaattta catgccatct atctggaatc agacacgcca gtctcgcgcg accagatcaa
    17101 acctgagcac ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg gtctggattt
    17161 taacccctcc tgtttttcgc atccgctaag cgccgatggc tttacgcttt cccatgccga
    17221 cgacagcatt cgccagttct ggattgatca ctgcaaagcc agccgtcgcg tttcggccta
    17281 ttttggcgag caactcggca caccatcggt gatgaacatc tggatcccgg atggtatgaa
    17341 agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt
    17401 gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc gttgagagca aattgtttgg
    17461 cattggcgcg gagagctaca cggttggctc caatgagttt tacctggggt atgccaccag
    17521 ccgccagacg gcgctgtgcc tggacgccgg gcacttccac ccgactgaag tgatttccga
    17581 caagatttcc gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gtcgtccggt
    17641 tcgctgggac agcgatcacg tagtgctgct ggatgatgaa acccaggcaa ttgccagtga
    17701 gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc cttgacttct tcgatgcctc
    17761 tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg
    17821 tgcgttgctg gaacctaccg ccgagctgcg caagttggaa gcggcgggcg attacactgc
    17881 gcgtcaggca ctgctggaag agcagaaatc gttgccgtgg caggcggtat gggaaatgta
    17941 ttgtcagcgt cacgataccc cagcgggtag cgaatggctg gagagcgtgc gggcttatga
    18001 gaaagaaatt ttgagtcagc gtgggtaaac actgccagat gcggcacgag cgccttatcc
    18061 ggcctacaga tcgtgcatct gacaacaccc catttacagg aacaccgaac atgcaaaaca
    18121 ttactcagtc ctggtttgtc cagggaatga tcaaagccac caccgacgcc tggctgaaag
    18181 gctgggacga gcgcaacggc ggcaacctga cgctacgcct ggatgacgct gacatcgcac
    18241 cgtatcacga caatttccac caacaaccgc gttatatccc gctcagccaa cccatgcctt
    18301 tactggcaaa tacaccgttt atcgtcaccg gctcgggcaa attcttccgt aacgtccagc
    18361 ttgatcctgc ggctaactta ggcgtcgtaa aagtcgacag cgacggcgcg ggctaccata
    18421 ttctctgggg gttaaccaac gaggccgttc caacttctga acttccggca cactttctct
    18481 cccattgcga acgcattaaa gccaccaacg gtaaagatcg ggtgatcatg cactgccatg
    18541 ccaccaacct gatcgccctc acctatgtac tggaaaacga cacggcggtc ttcactcgcc
    18601 aactgtggga aggcagcacc gagtgcctgg tggtattccc ggatggcgtt ggcattctgc
    18661 cgtggatggt gcccggcacg gacgaaatcg gtcaggcgac cgcacaagaa atgcaaaaac
    18721 attcgctggt gttgtggccc ttccacggtg tgttcggttc cggaccgaca ctggatgaag
    18781 cctttggttt gatagatacc gcagagaaat cagcggaggt gttagtcaaa atctactcga
    18841 tgggcggtat gaagcagacc attacccgtg aggagctgat tgcactcggt gagcgttttg
    18901 gcgttacgcc actggccagt gcgctggcac tttaatatcc caacagatat ccggcggtgt
    18961 gggaaacgtc gccggacaga tggatttgaa cttaaggaga gtcattatga gttttatgtt
    19021 ggcattaccg aaaattagcc tgcatggtgc tggggcaatt ggcgatatgg taaatctggt
    19081 tgcgcataaa cagtggggaa aagcactgat cgttaccgat gggcaactgg ttaagttggg
    19141 attactggac agcctgttta tcgcactgga tcaatacgaa atgtcttatc acttatttga
    19201 tgaggtattt cctaacccca ctgaagaact ggtgcaaaaa ggctacgctg catttcagga
    19261 tgccgcatgt gactacatta ttgctttcgg tggcggtagc ccaattgata ctgcaaaagc
    19321 cgtaaaaatc cttaccgcta atcctgcgcc ttctacggcg tactctggtg ttggtaaagt
    19381 gacaaatccc ggcgtaccgc tggtggcaat caatacgact gccggaacag ctgctgagat
    19441 gaccagcaat gcggtgatca tcgacaccgc gcggcaggta aaagaggtca ttatcgaccc
    19501 gaatattatt cccgatattg cggtggatga tgccagtgta atgctggaga tcccggcctc
    19561 ggttaccgcc gccacgggta tggatgcact gactcacgca gttgaagctt atgtttctgt
    19621 tggtgcacat ccattaaccg acgctaacgc actggaagct atccgtttaa ttacgctgtg
    19681 gctgccaaaa gcggtagatg atggtcataa tctggaagct cgtgaacaaa tggcattcgc
    19741 ccagtacctg gcgggtatgg catttaacag tgctggtctg ggccttgttc atgcacttgc
    19801 gcatcagccc ggcgccaccc acaacctgcc gcatggtgtg tgcaatgcta tcttgttgcc
    19861 agttattgaa aactttaacc gcccgaatgc catcgcacgc tttgcccgca ttgctcaggc
    19921 aatgggtgtc gatacgcgtg atatgagtga agaagcggca agtactgaag caattaacgc
    19981 tatccgcgca ttaagccatc gcgtgggtat tccggcagga ttcagccagc ttggcatcac
    20041 caaagaagat attgaaggct ggttagataa agccctggct gacccgtgtg tgccatgtaa
    20101 cccgcgtacc gccagccgcg acgaggtgcg cgagctgtat ctggaggcat tatgatccgt
    20161 aaggcatttg tgatgcaggt taaccccgac gctcacgaag agtatcaacg tcggcataac
    20221 cccatctggc cggaactgga agcagtgttg aaatctcacg gtgcgcataa ctacgccatt
    20281 tacctcgaca aagcgcgtaa tctgctgttt gccacggtag agattgaatc tgaagagcgc
    20341 tggaatgcgg ttgccagtac tgatgtttgc caacgttggt ggaaatatat gaccgacgtc
    20401 atgcccgcga acccggataa cagcccggtg agtagcgaac tgcaagaagt gttttacctg
    20461 ccgtaattgc ctaatgtgcc ggagacgcct ccggtacaat atttttaatt tcgatttgaa
    20521 aaataaacat attacacctt cagttaatat atctcggtaa cttatatttc cccgatgcat
    20581 tgtgagtaaa tcacaaaaat aatgaataac tcattaatga ttcatgtggt ttatttaaat
    20641 aaccattatg tgcattactc cgcaaatctg gcctttcact ctgtctcaaa ttgtcattgt
    20701 ttaccgacat atcacctctc atcacttaat cgctccacca ttacaattga ttaaaatata
    20761 ttttatggag tccgattaat ggcagctctt actgcaagct gtattgacct gaatattcag
    20821 ggcaatggcg cttattccat tctgaagcag ttggcgacaa tggcgttaca aaacggtttt
    20881 atcaccgact cacaccagtt cctgcaaacc ctgctgctgc gcgaaaaaat gcactctact
    20941 ggatttggtt ccggcatcgc cgtgccgcac ggtaaaagcg cctgcgttaa acaaccgttc
    21001 gtgttattcg cccgcaaagc gcaggccatt gactggaaag ccagcgacgg cgaagacgtc
    21061 aattgctgga tctgcctcgg cgtgccgcaa agtggcgaag aggatcaggt caaaatcatc
    21121 ggcacactgt gtcgcaaaat tatccatcag gattttatca accaactgcg acagggcgac
    21181 gccgaccagg tacttgcttt gttaaatcaa accctcagct cataaggaag tggcgatgga
    21241 gtcatcctta cgtattgtcg cgatcaccaa ctgccccgcc gggatcgctc acacctacat
    21301 ggtggcggaa gccctggaac agaaagcccg ttctctcggc cataccataa aagtggaaac
    21361 tcaagggtcc agcggcgttg aaaaccgctt atccagcgaa gagattgccg ctgccgatta
    21421 cgtcattcta gctaccgggc gtggcctgag cggtgatgac cgggcgcggt ttgccgggaa
    21481 gaaagtttat gagattgcca tctcccaggc gctgaaaaat atcgaccaga ttttcagcga
    21541 attaccaaca aactcgcagc tttttgccgc agatagcggc gtgaagctcg gcaaacagga
    21601 agtgcaaagc ggcagcgtga tgagccacct gatggcgggc gtctctgcgg cgctgccatt
    21661 tgtcatcggc ggcggtattc tggtggcgct tgccaacatg ctggtgcagt tcggtttgcc
    21721 atataccgat atgtcgaaag gtgcgccgtc gtttacctgg gtagttgaat ccatcggtta
    21781 cctcggcttc acctttatga tccccatcat gggcgcttat attgcctcgt cgattgccga
    21841 taaacccgct ttcgctccgg catttctggt ttgctatctg gcgaacgata aagccctgct
    21901 cggcacccag tcaggcgcgg gcttcctcgg cgcggtggtg ctggggctgg cgattggcta
    21961 tttcgtcttc tggttccgta aagtccgtct tggcaaagca ttacaaccgc tgctcggctc
    22021 catgcttatc ccattcgtta ctctgctggt ttttggcgtg ctcacttact acgttatcgg
    22081 gccggtgatg tccgacctca tgggcgggct gctccacttc ctgaatacca ttcctccatc
    22141 aatgaagttt gcggcagcgt ttctggtcgg tgcgatgctg gcgttcgata tgggcggccc
    22201 catcaataaa accgcctggt tcttctgctt ctcactgctg gaaaaacaca tttatgactg
    22261 gtacgccatc gtcggcgttg tcgcgctgat gccgcccgtc gcggcgggtc ttgcgacgtt
    22321 cattgcgccc aaactgttta cccgacagga aaaagaggct gccagtagtg ccattgtggt
    22381 cggtgccaca gtcgcaaccg aaccggctat tccttacgct ctggcagctc cgttgccgat
    22441 gattaccgcc aatacgctgg cgggcggtat taccggcgtg ctggtgatcg ctttcggaat
    22501 caaacgtctg gcacctggtt tgggtatctt tgacccgctg atcggcctga tgtcaccggt
    22561 aggttcgttt tatctggtgc tggcgattgg cctcgcgctg aacatttcat tcattatcgt
    22621 cctgaaagga ttgtggttac gccgcaaagc gaaagccgcg caacaggagc ttgtccatga
    22681 acattgagtt actgcaacag ttgtgcgaag ccagcgccgt tagcggcgat gaacaggaag
    22741 ttcgcgacat tctgataaac acgctggaac cttgcgtgaa tgaaatcacc tttgatgggc
    22801 tgggcagctt tgtcgcccgt aagggaaata aagggccgaa agttgccgtt gtcgggcata
    22861 tggatgaagt cggctttatg gtcacccaca tcgacgagag cggttttctg cgtttcacca
    22921 ccattggtgg ctggtggaat cagtcgatgc tcaaccaccg ggtaaccata cgcacacaca
    22981 agggagtgaa aatccctggt gtgattggtt ccgtcgcgcc tcatgcgtta acggaaaagc
    23041 aaaagcaaca gccgcagtca tttgatgaga tgttcattga tattggcgca aacagtcgcg
    23101 atgaggtgga aaaacgcggt gtggcgatgg gcgattttat tagcccggaa gccaattttg
    23161 cctgctgggg cgaagataaa gtggtcggca aggcgttgga taaccgcatc ggctgcgcaa
    23221 tgatggctga gctactacag acggtgaata atcccgaaat tacgctgtat ggcgttggca
    23281 gtgtggaaga agaagttggg ctacgcgggg cgcaaacctc ggctgaacac attaaaccgg
    23341 atgtggtgat cgtgctggac accgctgtcg cgggcgatgt tccaggcatt gataacatta
    23401 aatatccgct gaaactgggc aacgggccgg ggttgatgct gtttgacaag cgctacttcc
    23461 ccaaccagaa actggtagca gcgttaaaaa gctgtgccac gcagaatgat ttaccgctgc
    23521 aattttccac catgaaaacc ggtgcgacgg atggcgggcg ctacaacgtg atgggcggtg
    23581 ggcgtccggt tgtcgcgctg tgtttgccaa cgcgttatct gcacgccaac agcggcatga
    23641 tttcaaaagc cgattacgat gctctgctca cgctgatacg gggttttctg accaccttaa
    23701 ccgcggaaaa agtcagcgcg tttagccagt tccgtcaggt ggattaatgc taaacgaacg
    23761 ccagttaaag attgtcgatc tgctggagca acagccgcgc acgcctggcg agctggcgca
    23821 acagactggc gtttctggca ggaccatact gcgtgatatt gactatctca actttaccct
    23881 taacggcaaa gcccgcattt ctgacagtgg tagtgcgggc tatcagctgg aaatcttcga
    23941 gcgccgcagc ttttttcagt tgctgcaaaa gcacgataac gacgatcggc tgctggcact
    24001 gttattactg aataccttta ctccccgtgc gcaactcgcc tcggcgctta atttgccgga
    24061 aacgtgggtc gcagagcgtc tgccccggtt aaaacagcgt tatgaacgca cttgctgcct
    24121 ggccagtcgc cccggtttgg gccatttcat tgatgagaca gaagaaaaac gcgttatatt
    24181 gctggcgaac ttgctgcgca aagatccgtt tttaattccg ctggcgggcg taacacgaga
    24241 caaccttcag catttatcca cggcctgcga caaccaacac cgctggccgc tcatgcaggg
    24301 tgattatctc tccagcctga ttctggcgat ttacgccctg cgtaatcaac tgaccgatga
    24361 gtggccgcaa tatcccggtg acgagataaa acaaatcgtt gaacatagcg gtctgtttct
    24421 tggtgataac gctgtaagaa ccctgacagg tttgatagag aaacagcatc agcaagcgca
    24481 gataatttct gccgataatg tgcagcggtt gctgcaaagg gtgccgggca tcgcgtcatt
    24541 gaatattatt gatacgcagc tggttgagaa cattaccggg catttattac gttgccttgc
    24601 cgcaccggtg tggattgctg agcaccgcca aagcagcatg aataacctga aagccgcctg
    24661 gcctgcggcc tttgatatga gtctgcactt tattacgcta ctgcgtgaac agctcgatat
    24721 tccccttttc gacagcgatc tgatcggttt gtattttgcc tgtgcgctgg aacggcatca
    24781 aaacgaacgc cagccgatca ttttgctctc ggaccagaac gcgattgcca ctattaatca
    24841 gctcgccatt gagcgcgatg ttttacattg tcgggtgatt attgcccgta gcttaagcga
    24901 acttgttgcc attcgcgaag agattgagcc gttattgatc attaacaaca gccattattt
    24961 actggatgac gcggtaaata attacatcac cgtaaaaaat atcattacgg ctgccggtat
    25021 cgaacaaata aaacattttc tggcgacggc atttattcgc caacagccgg agcgtttttt
    25081 ctctgctccc ggaagttttc attattcgaa tgtacgcggt gaaagctggc aacatattac
    25141 ccggcaaatt tgtgcgcaat tagtcgcaca acaccatatt accgccgatg aagcacaacg
    25201 catcatcgcc cgcgaaggcg aaggtgaaaa cctgattgtt aatcgcctcg ccatcccaca
    25261 ttgctggagc gaacaggagc gacgttttcg tggatttttt attaccctcg cccaaccagt
    25321 tgaggtgaat aacgaagtca ttaaccatgt cttgatcgcc tgcgccgccg ccgatgcgcg
    25381 tcatgagcta aaaatattta gctatctggc aagcatattg tgtcagcatc cggcggagat
    25441 tattgccggg ttaacaggat atgaggcatt tatggagtta cttcacaagg ggtgaactgt
    25501 gtcggatgcg ccgcatccga cactgcattt atcatcaaaa acgtttaacg caggctatta
    25561 tagtcatcaa ccatttcgtt gtactcgcgt aatgctctcg ggaaagagtc ttcgaccatc
    25621 cagcttgagt ttgcgtcctg aagttgctct ttatcccagt cagagtacgg gactttatct
    25681 ctgatgcggc gaacgtattt tttagcctcg agttgatact ggcctgccga attaataaac
    25741 gagaaattca tgccgccttt atccgcttct ttggcctgcg ccactagcgt ttccagttca
    25801 gacactttct tcattgccgc atcaacatca aacttctctt gcgatatcag gttattaatt
    25861 tgtttcgctg aaatcatgac tgccagagag tagtagtgga atgttttccc ttctctttct
    25921 tcaatatttt tcagttccgc aagctggcgc ttatcgttaa tttcctgaat cgccgcatga
    25981 taagattctg ctacaggttc aaaggcttcc agatttttta agaatgtctg gtgcagcgtt
    26041 ttccctttcg caaacgcatc gtctttataa ttttcctggg tgtaatattt atccatttcg
    26101 ttaatggtat tacccaacgc cacggcagca tcgatataac tcaccgccac gccatcaatt
    26161 ggttgcagcg caggcgttaa cgccacagca ctttttacgc ctttttcaca ctcagcgagg
    26221 ttggattcac taatgccgta gatgccataa acagtacgct cttcaccggt aggcccctgt
    26281 ttaaaatctt tcagccagtc agcataacgc gccagactgc tctgtacagg gatctgtaac
    26341 ttgttatagc acttgatata gacatccatt ttttcgtcga aaaccgcttg ttcatcgacc
    26401 accggttgcg caggtgtttc aggtttagct tctgcttttg caacgggagc ttcaggagct
    26461 ggtgcggcag gttg
//
LOCUS       DAZKGS010000057        26464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKGS010000057 DAZKGS010000000
VERSION     DAZKGS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..221
                     /gene="tuf"
                     /locus_tag="ACHOQY_002289"
     CDS             <1..221
                     /gene="tuf"
                     /locus_tag="ACHOQY_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9678377.1"
                     /translation="FFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            404..598
                     /gene="bfd"
                     /locus_tag="ACHOQY_002290"
     CDS             404..598
                     /gene="bfd"
                     /locus_tag="ACHOQY_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9678378.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            671..1147
                     /gene="bfr"
                     /locus_tag="ACHOQY_002291"
     CDS             671..1147
                     /gene="bfr"
                     /locus_tag="ACHOQY_002291"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9678379.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1144..1611)
                     /locus_tag="ACHOQY_002292"
     CDS             complement(1144..1611)
                     /locus_tag="ACHOQY_002292"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9678380.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1990..2301
                     /gene="rpsJ"
                     /locus_tag="ACHOQY_002293"
     CDS             1990..2301
                     /gene="rpsJ"
                     /locus_tag="ACHOQY_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9678381.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2334..2963
                     /gene="rplC"
                     /locus_tag="ACHOQY_002294"
     CDS             2334..2963
                     /gene="rplC"
                     /locus_tag="ACHOQY_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9678382.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2974..3579
                     /gene="rplD"
                     /locus_tag="ACHOQY_002295"
     CDS             2974..3579
                     /gene="rplD"
                     /locus_tag="ACHOQY_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9678383.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3576..3878
                     /gene="rplW"
                     /locus_tag="ACHOQY_002296"
     CDS             3576..3878
                     /gene="rplW"
                     /locus_tag="ACHOQY_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9678384.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3896..4717
                     /gene="rplB"
                     /locus_tag="ACHOQY_002297"
     CDS             3896..4717
                     /gene="rplB"
                     /locus_tag="ACHOQY_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9678385.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4734..5012
                     /gene="rpsS"
                     /locus_tag="ACHOQY_002298"
     CDS             4734..5012
                     /gene="rpsS"
                     /locus_tag="ACHOQY_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9678386.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5027..5359
                     /gene="rplV"
                     /locus_tag="ACHOQY_002299"
     CDS             5027..5359
                     /gene="rplV"
                     /locus_tag="ACHOQY_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9678387.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5377..6078
                     /gene="rpsC"
                     /locus_tag="ACHOQY_002300"
     CDS             5377..6078
                     /gene="rpsC"
                     /locus_tag="ACHOQY_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9678388.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6091..6501
                     /gene="rplP"
                     /locus_tag="ACHOQY_002301"
     CDS             6091..6501
                     /gene="rplP"
                     /locus_tag="ACHOQY_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9678389.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6501..6692
                     /gene="rpmC"
                     /locus_tag="ACHOQY_002302"
     CDS             6501..6692
                     /gene="rpmC"
                     /locus_tag="ACHOQY_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9678390.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6692..6946
                     /gene="rpsQ"
                     /locus_tag="ACHOQY_002303"
     CDS             6692..6946
                     /gene="rpsQ"
                     /locus_tag="ACHOQY_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9678391.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7111..7482
                     /gene="rplN"
                     /locus_tag="ACHOQY_002304"
     CDS             7111..7482
                     /gene="rplN"
                     /locus_tag="ACHOQY_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9678392.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7493..7807
                     /gene="rplX"
                     /locus_tag="ACHOQY_002305"
     CDS             7493..7807
                     /gene="rplX"
                     /locus_tag="ACHOQY_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9678393.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7822..8361
                     /gene="rplE"
                     /locus_tag="ACHOQY_002306"
     CDS             7822..8361
                     /gene="rplE"
                     /locus_tag="ACHOQY_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9678394.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8376..8681
                     /gene="rpsN"
                     /locus_tag="ACHOQY_002307"
     CDS             8376..8681
                     /gene="rpsN"
                     /locus_tag="ACHOQY_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9678395.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8715..9107
                     /gene="rpsH"
                     /locus_tag="ACHOQY_002308"
     CDS             8715..9107
                     /gene="rpsH"
                     /locus_tag="ACHOQY_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9678396.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEGFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9120..9653
                     /gene="rplF"
                     /locus_tag="ACHOQY_002309"
     CDS             9120..9653
                     /gene="rplF"
                     /locus_tag="ACHOQY_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9678397.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9663..10016
                     /gene="rplR"
                     /locus_tag="ACHOQY_002310"
     CDS             9663..10016
                     /gene="rplR"
                     /locus_tag="ACHOQY_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9678398.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10031..10534
                     /gene="rpsE"
                     /locus_tag="ACHOQY_002311"
     CDS             10031..10534
                     /gene="rpsE"
                     /locus_tag="ACHOQY_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9678399.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10538..10717
                     /gene="rpmD"
                     /locus_tag="ACHOQY_002312"
     CDS             10538..10717
                     /gene="rpmD"
                     /locus_tag="ACHOQY_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9678400.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10721..11155
                     /gene="rplO"
                     /locus_tag="ACHOQY_002313"
     CDS             10721..11155
                     /gene="rplO"
                     /locus_tag="ACHOQY_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9678401.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11163..12494
                     /gene="secY"
                     /locus_tag="ACHOQY_002314"
     CDS             11163..12494
                     /gene="secY"
                     /locus_tag="ACHOQY_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9678402.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12526..12642
                     /gene="rpmJ"
                     /locus_tag="ACHOQY_002315"
     CDS             12526..12642
                     /gene="rpmJ"
                     /locus_tag="ACHOQY_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9678403.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12789..13145
                     /gene="rpsM"
                     /locus_tag="ACHOQY_002316"
     CDS             12789..13145
                     /gene="rpsM"
                     /locus_tag="ACHOQY_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9678404.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13162..13551
                     /gene="rpsK"
                     /locus_tag="ACHOQY_002317"
     CDS             13162..13551
                     /gene="rpsK"
                     /locus_tag="ACHOQY_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9678405.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13585..14205
                     /gene="rpsD"
                     /locus_tag="ACHOQY_002318"
     CDS             13585..14205
                     /gene="rpsD"
                     /locus_tag="ACHOQY_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9678406.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14231..15220
                     /gene="rpoA"
                     /locus_tag="ACHOQY_002319"
     CDS             14231..15220
                     /gene="rpoA"
                     /locus_tag="ACHOQY_002319"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9678407.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15261..15644
                     /gene="rplQ"
                     /locus_tag="ACHOQY_002320"
     CDS             15261..15644
                     /gene="rplQ"
                     /locus_tag="ACHOQY_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9678408.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15751..16119
                     /locus_tag="ACHOQY_002321"
     CDS             15751..16119
                     /locus_tag="ACHOQY_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9678409.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16130..16555
                     /gene="zntR"
                     /locus_tag="ACHOQY_002322"
     CDS             16130..16555
                     /gene="zntR"
                     /locus_tag="ACHOQY_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9678410.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16611..16829
                     /gene="arfA"
                     /locus_tag="ACHOQY_002323"
     CDS             16611..16829
                     /gene="arfA"
                     /locus_tag="ACHOQY_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9678411.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16826..17236)
                     /gene="mscL"
                     /locus_tag="ACHOQY_002324"
     CDS             complement(16826..17236)
                     /gene="mscL"
                     /locus_tag="ACHOQY_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9678412.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17366..18742)
                     /gene="trkA"
                     /locus_tag="ACHOQY_002325"
     CDS             complement(17366..18742)
                     /gene="trkA"
                     /locus_tag="ACHOQY_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9678413.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18764..20053)
                     /gene="rsmB"
                     /locus_tag="ACHOQY_002326"
     CDS             complement(18764..20053)
                     /gene="rsmB"
                     /locus_tag="ACHOQY_002326"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9678414.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20099..21046)
                     /gene="fmt"
                     /locus_tag="ACHOQY_002327"
     CDS             complement(20099..21046)
                     /gene="fmt"
                     /locus_tag="ACHOQY_002327"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9678415.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21061..21570)
                     /gene="def"
                     /locus_tag="ACHOQY_002328"
     CDS             complement(21061..21570)
                     /gene="def"
                     /locus_tag="ACHOQY_002328"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9678416.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21700..22824
                     /gene="dprA"
                     /locus_tag="ACHOQY_002329"
     CDS             21700..22824
                     /gene="dprA"
                     /locus_tag="ACHOQY_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP9678417.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22796..23269
                     /gene="smg"
                     /locus_tag="ACHOQY_002330"
     CDS             22796..23269
                     /gene="smg"
                     /locus_tag="ACHOQY_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9678418.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23298..23840
                     /locus_tag="ACHOQY_002331"
     CDS             23298..23840
                     /locus_tag="ACHOQY_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9678419.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23845..24417
                     /gene="tsaC"
                     /locus_tag="ACHOQY_002332"
     CDS             23845..24417
                     /gene="tsaC"
                     /locus_tag="ACHOQY_002332"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9678420.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24422..25240
                     /gene="aroE"
                     /locus_tag="ACHOQY_002333"
     CDS             24422..25240
                     /gene="aroE"
                     /locus_tag="ACHOQY_002333"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9678421.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25237..25494
                     /locus_tag="ACHOQY_002334"
     CDS             25237..25494
                     /locus_tag="ACHOQY_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9678422.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25470..26024)
                     /locus_tag="ACHOQY_002335"
     CDS             complement(25470..26024)
                     /locus_tag="ACHOQY_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9678423.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6803 a         6286 c         6860 g         6515 t
ORIGIN      
        1 cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg actggtacca
       61 tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa atggttgtta
      121 ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt gaaggcggcc
      181 gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt aattagtttt
      241 gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct cgaaatattt
      301 agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg taatcataac
      361 cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg tttgtttgtg
      421 taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc cgcaatcttt
      481 ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca ttcgcgccgc
      541 gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga ttgcctgaag
      601 accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt ggaagcggag
      661 ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac tattgggaaa
      721 tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa actggggcct
      781 gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac acgctgataa
      841 atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt taggcaaact
      901 ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag aactggaagg
      961 cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact atgttagccg
     1021 tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc tcgaaactga
     1081 gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa taaaagttaa
     1141 agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag ccgccagaaa
     1201 tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca gaccaacaac
     1261 tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga aggtccaggt
     1321 atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc cttctttatg
     1381 acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa taatcgcccc
     1441 ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa gacctgacca
     1501 gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc ataaaaaaag
     1561 taggacagat aaacaagcgt acaaaatcag aaagggaagg ttagcgtcca taaaacatcc
     1621 tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga gaaatcgctg
     1681 atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa tttcgtggaa
     1741 tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt caggctcaga
     1801 tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga acctcagtta
     1861 gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa cggttacact
     1921 ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa taattggagc
     1981 tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga tcatcgtctg
     2041 atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc gcaggtccgt
     2101 ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc cccgcacgtc
     2161 aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt tgacatcgtt
     2221 gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc cggtgtagac
     2281 gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa acaatgattg
     2341 gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc gtttctatcc
     2401 cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac ctggctaacg
     2461 atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt gtgaccaagc
     2521 ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg tgggaattcc
     2581 gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa ctgtttgctg
     2641 acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca ggtaccgtta
     2701 agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct caccgcgttc
     2761 cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag aaaatggcag
     2821 gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc gttgacgctg
     2881 agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc gacctgatcg
     2941 ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa agacgcgcag
     3001 agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc gctggttcac
     3061 caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca gaagactcgt
     3121 gctgaagtaa ctggttctgg taaaaaaccg tggcgccaga aaggcaccgg ccgtgcgcgt
     3181 tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc tgctcgtccg
     3241 caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa aagcatcctg
     3301 tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt agaagcgccg
     3361 aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt gctgatcatc
     3421 accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa ggttgacgta
     3481 cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt cgtaatgact
     3541 gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa cgtctgctga
     3601 aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa aaatccaaca
     3661 ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct gctgtgcaga
     3721 aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg aaagttaaac
     3781 gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc accctgaaag
     3841 aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta atacaatggc
     3901 agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag tggttaaccc
     3961 tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca aatccggtgg
     4021 tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca agcaggctta
     4081 ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg aacgtcttga
     4141 gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg gtgaacgccg
     4201 ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg gcgttgatgc
     4261 tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt ctactgttca
     4321 taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg gtacttacgt
     4381 tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg gtgaaatgcg
     4441 taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg agcatatgct
     4501 gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga ccgttcgcgg
     4561 taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta actttggtaa
     4621 gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca gcaacaagcg
     4681 tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa gccatgccac
     4741 gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta gagaaagcgg
     4801 tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg atctttccta
     4861 acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg gtatttgtaa
     4921 ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact tatcgcggcc
     4981 acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg aaactatcgc
     5041 taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg acctgattcg
     5101 cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga aagcggctgt
     5161 actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg atggcgctga
     5221 cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca tgaagcgcat
     5281 tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc acatcactgt
     5341 ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt acatcctaat
     5401 ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa caccaaagaa
     5461 ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa ggaactggct
     5521 aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg tgtaaccatt
     5581 cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga aaaactgcgt
     5641 aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga agttcgtaag
     5701 cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga acgtcgcgtt
     5761 atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg cgctaaaggt
     5821 attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac cgaatggtac
     5881 cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa cacctctgaa
     5941 gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg cgagatcctg
     6001 ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa aaagcagcag
     6061 cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac aaaattccgt
     6121 aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag cttcggcagc
     6181 ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga agcagcacgt
     6241 cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt gttcccggac
     6301 aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg taacgtggag
     6361 tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg tgttccggaa
     6421 gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa aaccaccttt
     6481 gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc gttgaagagc
     6541 tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg caggctgcaa
     6601 gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc gcacgcgtta
     6661 agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta ctctgcaagg
     6721 tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac gttttgtgaa
     6781 acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac atgacgagaa
     6841 caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc tgtccaagac
     6901 taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca gtacactctc
     6961 tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat atccttgaag
     7021 cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt ttcgggtctc
     7081 agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat gctgaacgtc
     7141 gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg ctcgcaccgt
     7201 cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat tccgcgtggt
     7261 aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa gggtgttcgt
     7321 cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct gaacaacaac
     7381 agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct tcgtagtgag
     7441 aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa tcatggcagc
     7501 gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag gtaaacgcgg
     7561 taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca acctggttaa
     7621 gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg aaaaagaagc
     7681 cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg ctgaccgtgt
     7741 aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca gcgaaactat
     7801 caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga cgaagtagtt
     7861 aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg ggtcgagaag
     7921 atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct ggataacgca
     7981 gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc acgcaaatct
     8041 gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac tctgcgtggc
     8101 gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg tatccgtgac
     8161 ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat gggtgtccgt
     8221 gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg tggtttggat
     8281 attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct ggctgccttt
     8341 gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca atgaaagcac
     8401 gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct gaactgaaag
     8461 cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt ctcaagctgc
     8521 agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt caaacaggtc
     8581 gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt gaagccgcta
     8641 tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca attgaatcac
     8701 gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc cgtatccgta
     8761 acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg aaagtggcaa
     8821 tcgccaacgt gctgaaggaa gaaggtttta ttgaaggttt taaagttgaa ggcgacacca
     8881 agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta gaaagcattc
     8941 agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg ccgaaagtta
     9001 tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact gatcgtgcag
     9061 cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg gaggaaaaaa
     9121 tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta aaaatcaacg
     9181 gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc aacgatgctg
     9241 ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt tacgcagacg
     9301 gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc ggtgttaccg
     9361 aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg gttaaaggca
     9421 atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg cctgcgggta
     9481 tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat aagcaggtga
     9541 tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat aaaggcaagg
     9601 gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag taaggtaaca
     9661 ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc aagctccagg
     9721 agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac gcacaggtaa
     9781 ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa gctatcgctg
     9841 aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa gctgtcgctg
     9901 aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg ttccaatatc
     9961 atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag ttctaaggta
    10021 gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa agctgatcgc
    10081 ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca cagctctgac
    10141 tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc gtgaagttcc
    10201 agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg tcgcgctgaa
    10261 taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg tattcatgca
    10321 gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg ttctggaagt
    10381 cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga tcaacgtggt
    10441 tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg ctgccaagcg
    10501 tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta ttaaaattac
    10561 tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc ttggcctggg
    10621 tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc gcggtatgat
    10681 caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa atactctgtc
    10741 tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg gttctggcct
    10801 cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg gtggcgtacg
    10861 tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat tcggcttcac
    10921 ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta aagtagaagg
    10981 cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc agatcgagtt
    11041 cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg gcctgcgtgt
    11101 tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg aataagtagc
    11161 agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta ggcgagctga
    11221 aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc tcttttattc
    11281 cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag cgaggcacca
    11341 tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct atctttgctc
    11401 tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg gtagttcacc
    11461 caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc agccagtaca
    11521 cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct accggtctgc
    11581 cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc tacttcaccg
    11641 ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa cagattactg
    11701 aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc gcgggactcc
    11761 cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac ttcctcgtgt
    11821 tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt gttgagcgtg
    11881 gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt gtctatgctg
    11941 cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg gcaatcttcg
    12001 cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc ggtactggtt
    12061 ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt tatgtgttac
    12121 tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt ttcaacccgc
    12181 gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt cgtccgggag
    12241 agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt ggtgcgctgt
    12301 atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa gtaccgttct
    12361 acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt atggctcaag
    12421 tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg aacctgaaag
    12481 gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa agttcgtgct
    12541 tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt catccgtgtg
    12601 atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg catatttttc
    12661 ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg tctgtgcgtt
    12721 tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt aaatagtagg
    12781 agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat gccgtaatcg
    12841 cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg gctgcagcgg
    12901 gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac acgctgcgtg
    12961 acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc atgagcatca
    13021 agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt ctcccggttc
    13081 gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa ccgatcaaga
    13141 aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa acgtgtaaga
    13201 aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac catcgtgact
    13261 atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc cggtttccgt
    13321 ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg cgctgacgcc
    13381 gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg tccaggccgc
    13441 gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat tactgatgtg
    13501 actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata acgcctcgtt
    13561 ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct caagctgagc
    13621 cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga taccaagtgt
    13681 aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc tgactatggt
    13741 gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga gcgtcagttc
    13801 cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa cctgttggct
    13861 ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc cactcgtgca
    13921 gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt tgttaacatc
    13981 gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc gaagaagcag
    14041 tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac ctggctggaa
    14101 gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc tgatctgtct
    14161 gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt agtaccaaag
    14221 agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg ttgatatcga
    14281 gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg gctttggcca
    14341 tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt gcgcggtgac
    14401 cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg ttcaggaaga
    14461 tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg gcaaagatga
    14521 agttattctt accttgaata aatctggcat tggccctgtg actgcagccg atatcaccca
    14581 cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga ccgatgagaa
    14641 cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc cggcttctac
    14701 ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg acgcatgcta
    14761 cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac agcgtaccga
    14821 cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg aagaggcgat
    14881 tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact tacgtgatgt
    14941 acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc tgcgccctgt
    15001 tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag ctatccacta
    15061 tatcggtgat ctggtacaac gtaccgaggt tgagctcctt aaaacgccta accttggtaa
    15121 aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc tgggcatgcg
    15181 cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca ggttaaggtt
    15241 ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg aaccgcaaca
    15301 gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt catgaaatca
    15361 tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg ctgattactc
    15421 ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact cgtgataacg
    15481 agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt gccggtggtt
    15541 acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg gcttacatcg
    15601 agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa gcaacgtaaa
    15661 aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct acaatagctg
    15721 tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg ggcagagcgt
    15781 catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag cggcgaacca
    15841 ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta tcgcttgctg
    15901 aagaatgccg gttgcttacc gccagaactt gagcagcgga gggaagcaat tcagcttctg
    15961 gatatcctga gaggtatccg tcacgatgat ccgcaatatc aagaggttag ccgtcgattg
    16021 tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt acgcggcgat
    16081 tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta tgtatcgcat
    16141 tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt acgaaaaaca
    16201 gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata ccgaaagcga
    16261 tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc tggagtcgat
    16321 ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg agtcaaaagg
    16381 cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc agagtatgca
    16441 gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca gtgtttattg
    16501 ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct gttgattttt
    16561 tgcactgaca ggattacact cgcgccgtaa aataaccaac tggagttttt atgagccgat
    16621 atcagcatac taaagggcag ataaaggata atgcgataga agcattacta catgatccct
    16681 tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg cgaaaaggaa
    16741 aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac ttttttacca
    16801 ctggtcttct gctttcaggc gcttgttaag agcggttatt ctgctctttc agcaaatcac
    16861 gaatttctgc cagtaatact tcttctttag ttggtgcagg tgcggcagct ggttcttctt
    16921 ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag gccacaatca
    16981 gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca gggatatccc
    17041 cctgcgcatc gcgtagcgtg acagcaaact gtttaaaatc gatcccgcca attaataagc
    17101 ccagaggagg catgatgata tcggcaacca gtgaagagac aatcttcccg aatgccgcac
    17161 cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat tcgcgaaatt
    17221 ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc taacaaatgt
    17281 taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa gggaaataat
    17341 aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggttgg aagagtcttt
    17401 cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg ccttgctcaa
    17461 tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct ccaataatcg
    17521 ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag gtgctttcat
    17581 caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag gaaacaccaa
    17641 caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt tgtggtgaaa
    17701 tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc tggatcaata
    17761 ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata ttggcctcgt
    17821 catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg gccagcagtt
    17881 cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc gccagttcgg
    17941 cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc agacgacgcg
    18001 ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac ggtttttcca
    18061 gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata aagaacactt
    18121 catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg tggcggaaaa
    18181 ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa agtgcgttac
    18241 caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta ccctcagcga
    18301 agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta tcgataacca
    18361 actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat agcttatcgg
    18421 catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg ttgaaaagtg
    18481 agtaggctac ctggcaggca accatattgg tttcatctga actggttaca gcaaccagca
    18541 tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca tgcccctgca
    18601 cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg gtatcgacaa
    18661 cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca acctggccgg
    18721 cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt ttgattagct
    18781 tagcgtaaaa gaagccgtcg ccctcttcgg taccaggcag attttgttta cccggttgct
    18841 ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag gctttaatct
    18901 gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt ccaccggatt
    18961 ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt tccgggatat
    19021 cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg gttgctgaac
    19081 aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg gaagggtagc
    19141 ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg tcgtaaacgc
    19201 gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt gccacctcaa
    19261 ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt tcaccgtttt
    19321 gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta acccatccgt
    19381 cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca gcatcagggt
    19441 aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc cagctgtcgc
    19501 gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta ttagcttcga
    19561 cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac caggaaggat
    19621 gcagataacg tgcatcactg gcataaaacc cggctaataa ctcttcttgc tgacgctgga
    19681 actggcgtaa tacaccgtta atcagcccct taagttgcgg gcgcttaatt gcgacagcgc
    19741 cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc agttgataca
    19801 caccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga cgggccatta
    19861 acttattaat cagccagtct aactgtgaaa gcgtacgcag tacgccaaag cacaactctt
    19921 gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga atgttgctta
    19981 atgattgccc ttgctcgacg atttgttcaa cggcctgtgc cgccatgcta cgtaaattac
    20041 gttgtttttt cataagtata aaaatgcccg gtaagaccgg gcttagaaga gtggactatc
    20101 agaccagacg gttgcccgga acaaaccatt cccgacgaga attcaggagg tcttgcgcgc
    20161 tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca tcaccagtcg
    20221 caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg ttggttgccg
    20281 tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc agccagctca
    20341 ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa agcgaccagt
    20401 caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt tcgtcctgaa
    20461 cttctggttt cgccgtgccg tctgctagtt gtttcaacgt ggtgataagc ccttgtgggc
    20521 caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta atcgggcagg
    20581 agagcttata aagcatgtca ccggtgtcta aaccaacatc catttgcata atggtcacac
    20641 cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg cgccagcgtg
    20701 gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc actgctttag
    20761 gcagaattaa gccataggcg acgacgacca taacatcagc ctgcagatcg gcgaccagtt
    20821 gctggctttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct ttttcctcag
    20881 ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt cggtctggct
    20941 gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga tgacgcgctg
    21001 caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta gttcttatcc
    21061 ttaagcccgg gctttcagac gatctagttt ttcaactttc tgacgaatac gttgttgttt
    21121 cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct catgctgaat
    21181 acagatggct aacagcccct ctgcttccag ttcaaatggt ttaccgtcgc ggtcaagggc
    21241 gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga tcgacaggca
    21301 accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga ttaacaccag
    21361 ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga tatcaacctg
    21421 ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat catcgacgat
    21481 acgctgtatt tctgcattca cttcttctac cggtttagca actttgcgaa gccgctcgtc
    21541 cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc aggagttaga
    21601 aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca ctgaccaatc
    21661 gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac agaaatttgg
    21721 ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat agctcactgg
    21781 ctggcaaaac agtcgcaaat tgatgcggtt ggattgcagc aaacagggct tacattgcgg
    21841 caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact ttgttggttg
    21901 gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc tcaacttctg
    21961 gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc gctgcattca
    22021 tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg atggggacgg
    22081 ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact ggcgcgtgga
    22141 atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat tgctgtattg
    22201 gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc cagtctgctt
    22261 gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct tgcttacaat
    22321 ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt ggtggaagcg
    22381 gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg gcgtgaagtt
    22441 tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg gttaataaaa
    22501 caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca atttggattg
    22561 cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca agacgtggca
    22621 ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt tgacgtcgtc
    22681 gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga actggagtta
    22741 gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc atgccatgtt
    22801 cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt tgcgtgtgga
    22861 tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag atatctacaa
    22921 tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag aaccgatgca
    22981 actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg aaagactgga
    23041 tgccagctgt cgtggttttc tgcttttcct tgagcaaatt caggtgctta accttgaaac
    23101 ccgtgaaatg gtgatagagc gagtgctcgc gctggatacc gcagagttcg acctggaaga
    23161 cctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa atgcgtacca
    23221 gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat catttcttta
    23281 attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat aatgagtcct
    23341 gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg tttcttggat
    23401 gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg gatggacata
    23461 ttgtcaaagt tctggagggg caggtttgcc ttgcatgtgg cgcaaatctg gtattacgcc
    23521 agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa cataccgaac
    23581 ttatcgataa accggacgaa acagcaatta catgccccca atgccggacg ggccatctgg
    23641 tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac ccggagtgtc
    23701 aatttgccat taacttcaaa cccatagctg gagaatgccc tgagtgtcat tatccactac
    23761 tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa caatgtggaa
    23821 agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc tatcgcagct
    23881 gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc cgttttcggt
    23941 gttgggtgcg atcctgatag cgaaacagca gtgatgagac tgttggagtt aaaacagcgt
    24001 ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa accctatatt
    24061 gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg gccaggtcct
    24121 gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg ccgctttgat
    24181 tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca ggcttatggt
    24241 aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg aacagtagac
    24301 gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac gggggggcgt
    24361 ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca ggggtaacat
    24421 aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc cattcattca
    24481 tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt tggcacccat
    24541 caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag gtgcgaatgt
    24601 gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg aacgggcagc
    24661 gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcctgc tgggtgacaa
    24721 taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc gtcctggttt
    24781 acgtattctg ctcatcggcg ctggtggagc atctcgcggc gtactactgc cacttctttc
    24841 cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag agttggctaa
    24901 attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg aaggtcatga
    24961 gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc cggcgatccc
    25021 gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga aaggaaaaac
    25081 tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg gtttaggaat
    25141 gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc ctgacgtaga
    25201 accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat ccagtttccg
    25261 gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct cgtgaatggc
    25321 atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac tggagatacg
    25381 ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga agaagcggaa
    25441 aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc ctgatccaga
    25501 tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc cttctcttca
    25561 tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc gctctccagc
    25621 cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc tactattgcg
    25681 ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt gcagccgtgg
    25741 agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt gccatctggg
    25801 ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt ggtgcgtgct
    25861 ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat ccccacatca
    25921 tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac gcgctgaccg
    25981 atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac ctcagcaata
    26041 aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt tagtatgcaa
    26101 aaaagcaccg ttttgtgtgc gaatgcagca aaaagggtga aaaaacaaca aacagaaaaa
    26161 aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta taatgcgcct ccgttgagac
    26221 gacaacgtga aacacttcac aagatggtcg taacaacgaa gagaaaaaat cctgaaatta
    26281 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag
    26341 ccgcgtcgca actgctcttt aacaatttat cagacaatct gtgtgggcac tcgaagatac
    26401 ggattcttaa cgtcgcaaga cgaaaaatga ataccaagtc tcaagagtga acacgtaatt
    26461 catt
//
LOCUS       DAZKGS010000058        26293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKGS010000058 DAZKGS010000000
VERSION     DAZKGS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1177
                     /locus_tag="ACHOQY_002336"
     CDS             <1..1177
                     /locus_tag="ACHOQY_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9678424.1"
                     /translation="LINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIM
                     SIMIAFLVARNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLF
                     VAVIVALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCL
                     TLISPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETV
                     FSEANLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAE
                     YRDLAKMSFIPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPP
                     VAYAVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1205..1516
                     /locus_tag="ACHOQY_002337"
     CDS             1205..1516
                     /locus_tag="ACHOQY_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9678425.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1572..3245
                     /locus_tag="ACHOQY_002338"
     CDS             1572..3245
                     /locus_tag="ACHOQY_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9678426.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3270..4709
                     /locus_tag="ACHOQY_002339"
     CDS             3270..4709
                     /locus_tag="ACHOQY_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9678427.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4766..4984)
                     /gene="marB"
                     /locus_tag="ACHOQY_002340"
     CDS             complement(4766..4984)
                     /gene="marB"
                     /locus_tag="ACHOQY_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9678428.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5016..5399)
                     /gene="marA"
                     /locus_tag="ACHOQY_002341"
     CDS             complement(5016..5399)
                     /gene="marA"
                     /locus_tag="ACHOQY_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9678429.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5419..5853)
                     /gene="marR"
                     /locus_tag="ACHOQY_002342"
     CDS             complement(5419..5853)
                     /gene="marR"
                     /locus_tag="ACHOQY_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9678430.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6065..6730
                     /gene="marC"
                     /locus_tag="ACHOQY_002343"
     CDS             6065..6730
                     /gene="marC"
                     /locus_tag="ACHOQY_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9678431.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6755..7945)
                     /gene="ydeA"
                     /locus_tag="ACHOQY_002344"
     CDS             complement(6755..7945)
                     /gene="ydeA"
                     /locus_tag="ACHOQY_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9678432.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8095..9209)
                     /gene="yneK"
                     /locus_tag="ACHOQY_002345"
                     /pseudo
     CDS             complement(8095..9209)
                     /gene="yneK"
                     /locus_tag="ACHOQY_002345"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(9287..10168)
                     /gene="yneJ"
                     /locus_tag="ACHOQY_002346"
     CDS             complement(9287..10168)
                     /gene="yneJ"
                     /locus_tag="ACHOQY_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9678433.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            10269..11657
                     /gene="sad"
                     /locus_tag="ACHOQY_002347"
     CDS             10269..11657
                     /gene="sad"
                     /locus_tag="ACHOQY_002347"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9678434.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            11721..12647
                     /gene="glsB"
                     /locus_tag="ACHOQY_002348"
     CDS             11721..12647
                     /gene="glsB"
                     /locus_tag="ACHOQY_002348"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9678435.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            12647..13006
                     /gene="yneG"
                     /locus_tag="ACHOQY_002349"
     CDS             12647..13006
                     /gene="yneG"
                     /locus_tag="ACHOQY_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9678436.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            13145..14563
                     /locus_tag="ACHOQY_002350"
     CDS             13145..14563
                     /locus_tag="ACHOQY_002350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9678437.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            14790..16241
                     /gene="uxaB"
                     /locus_tag="ACHOQY_002351"
     CDS             14790..16241
                     /gene="uxaB"
                     /locus_tag="ACHOQY_002351"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9678438.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            16448..17362
                     /gene="yneE"
                     /locus_tag="ACHOQY_002352"
     CDS             16448..17362
                     /gene="yneE"
                     /locus_tag="ACHOQY_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9678439.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(17366..17860)
                     /locus_tag="ACHOQY_002353"
                     /pseudo
     CDS             complement(17366..17860)
                     /locus_tag="ACHOQY_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(17859..18695)
                     /gene="lsrF"
                     /locus_tag="ACHOQY_002354"
                     /pseudo
     CDS             complement(17859..18695)
                     /gene="lsrF"
                     /locus_tag="ACHOQY_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            complement(18722..19744)
                     /gene="lsrB"
                     /locus_tag="ACHOQY_002355"
     CDS             complement(18722..19744)
                     /gene="lsrB"
                     /locus_tag="ACHOQY_002355"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP9678440.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            complement(19756..20748)
                     /gene="lsrD"
                     /locus_tag="ACHOQY_002356"
     CDS             complement(19756..20748)
                     /gene="lsrD"
                     /locus_tag="ACHOQY_002356"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP9678441.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSASDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            complement(20748..21776)
                     /gene="lsrC"
                     /locus_tag="ACHOQY_002357"
     CDS             complement(20748..21776)
                     /gene="lsrC"
                     /locus_tag="ACHOQY_002357"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP9678442.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            complement(21770..23305)
                     /gene="lsrA"
                     /locus_tag="ACHOQY_002358"
     CDS             complement(21770..23305)
                     /gene="lsrA"
                     /locus_tag="ACHOQY_002358"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP9678443.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            23554..24507
                     /gene="lsrR"
                     /locus_tag="ACHOQY_002359"
     CDS             23554..24507
                     /gene="lsrR"
                     /locus_tag="ACHOQY_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP9678444.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            24586..26176
                     /gene="lsrK"
                     /locus_tag="ACHOQY_002360"
                     /pseudo
     CDS             24586..26176
                     /gene="lsrK"
                     /locus_tag="ACHOQY_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
BASE COUNT         6685 a         6226 c         6587 g         6795 t
ORIGIN      
        1 attaattaac tttgccatat tatcgccaga cggttttatt gccggattac tgcatctgaa
       61 tagcattttc cccaaccttg aaaaagcaca agctattttt actccggtaa tgaatggttc
      121 tgtaaatatc atgtcaatta tgattgcttt cctggtcgcc aggaatatgg cgattagcta
      181 tgagcaagat gatcttttat gcggattaac ggcaatagga gcatttttta ttgtttatac
      241 gccatatcag atgatagatg ggcaagcatt cctgacgacc aaatatctcg gcgcgcaggg
      301 gttgtttgtt gctgttatcg ttgcattgat caccagtgaa atattttgtc gcttagctcg
      361 aaacccaaaa atcaccataa cgatgccggc agctgtaccg cctgcggtag cgcgttcatt
      421 taaagtttta ttgccaatat tttttgtcat ggtgttcttt tccgcactta attattgcct
      481 gacactgata tccccggcag gactaaacga tcttatttac acattaatcc agacgccgct
      541 caaacatatg ggaacgaata tctttgcggt aattatcctg ggggctgtgg gtaatttcct
      601 gtgggtgctg gggatccacg gacctaatac cacatcggca attcgagaaa ccgttttttc
      661 tgaggctaat ctggagaatc tctcctgggc cgctcaacat ggcactacct ggggcgcgcc
      721 atatccgatt acctggactt ctattaatga tgcattcgcc aactgcggcg gttcaggtat
      781 gacgttgggg ttattgttgg ctatttttat cgcttctaag cgtgcggaat accgtgatct
      841 ggcaaaaatg tcatttatcc ccggtatttt caatatcaat gaaccgataa tgtttgacct
      901 tcctattgta cttaacccca tcatgatggt gccgtttatt atggttccca ttgttaactg
      961 tgccattggt tacttctttg tttcgatgga aattattcca ccggttgctt atgccgtgcc
     1021 ctggactacg cccggacctt taattgcttt cctcggaacc ggggggaact ggctggcttt
     1081 actggttggt tttttatgtt taggtgtggc gacaatgatc tatttacctt ttgttattgc
     1141 cgccaacaaa gtcaataaca tgacaactaa cggataattc catttaccca ggaaggagat
     1201 tgtgatgttt gcagatgaag aacttgttat ggagttactg gtcaacgcgg ggcaggcacg
     1261 ttctgacgcg atggaagcca tacgttgtgc cgggcaaaaa gactggcagg gcgcaactca
     1321 gctaatggcc agttctgaat ctgcctgtct tcaggcacat aagattcaga ccgcattaat
     1381 tagtcaggat gaaggctgtg gcaaaataaa agttaattta attctgattc atgcacagga
     1441 tcatttgatg aatgcaatat tatgccagga tttagccagg gaaattattt cattaagaaa
     1501 agaactccat gcctgatttt ttcctccagt gcgatagctg gaggaaatta ttatactctt
     1561 aaggataagt catgaatata aagacgctta acgtcagcct tttgtctttt tctattatta
     1621 cagcattgtt tccattgaac gcgatggcaa caaaattaac catagagcag cgccttgaat
     1681 tgcttgaaaa tgaattgtcg caaaataaac aagagctgaa agcaacacag aatgaactgg
     1741 gagtatataa atcccgactt tcgaccttac aaaaaagcat cacggaaaac aaatataaat
     1801 cagcctcgct tgctgaaata tcagcgacat ctcccgtcgc tgataacatc aaaaatgaaa
     1861 acggtgaaca gaactcgttt accgcagcgc atactataaa tggatcgcag caagttgccg
     1921 ttattgaaag taaaggcgac aaaaccacta tcgaaagcgt gaccctgaaa gatatcagta
     1981 aatatataaa ggatgatatt gggttcagct atcaggggta cttccgctca ggctggggta
     2041 ctggtaatca cggctcacca caaacttatg cagcgggttc tctgggacgt tttggtaatg
     2101 agatgagcgg ttggtttgac ctgaccttaa atcagcgtgt ttataatcag gacggtaaaa
     2161 cggcaaatgc ggtcgttacc tatgatggca acgtgggtga gcagtataac gatgcctggt
     2221 ttggtgacag tgccaatgaa aatatcatgc agttcagcga tatttacctg acaacgcgag
     2281 gttttttacc cttcgcacca gaggcagact tctgggtcgg caaacataaa ctcccgcaat
     2341 atgagatcca gatgctggac tggaaaacct taaccacgga tgtcgccgcg ggtgtgggga
     2401 ttgaaaactg ggcacttggt gtagggctgt ttgatatgtc cttaagccgt gatgatgtcg
     2461 atgtttactc tcgtgatttt tcgcgtacca gccagatgaa taccaattct gtggatgttc
     2521 gttatcgcaa tatcccgtta tgggatgatg cgacattatc attaatggct aaatattccg
     2581 cacctaataa aacggatcaa caacaagata acgaaaatga caacagttat tttgaaatga
     2641 aagatagctg gatgctgact tctgttttac ggcaaaacct gcaacgcgat acgtttaatg
     2701 aatttacgtt acaggttgcc aataattcct atgccagcag ttttgccagt ttctcagatg
     2761 ccagtaatac gatggcgcat ggtcgctatt actatggtga tcataccaat gggatcgcct
     2821 ggcgtttaat ctctcagggc gagatgtatc ttactgacaa tattattatg gctaacgcgc
     2881 ttgtctattc tcacggcgaa gatgtttata gttatgaaag tggtgctcat agtgattttg
     2941 acagtattcg cactgtaata agaccggcct ggatctggaa tacatggaat cagacggggc
     3001 ttgaattagg ctggtttaag caacagaaca aaactcagca gggtgtaaca ctaaatgaat
     3061 cggcttataa aacgacactc tggcatgcat tgaaagtggg tgaaagcatt ttaggttcac
     3121 gaccagaaat tcgcttctat ggaacgtata tcaatattct ggataacgaa ttatcgaatt
     3181 ttaagtttaa tgagaacagc aaagacgagt ttatggccgg catccaggcg gaagtctggt
     3241 ggtaatctat tttagttaag gagttgtgta tgtcaggatt taaaaaaggt tttttatggg
     3301 gcggtgcggt agccgcgcat cagttggaag gtggctggaa tgaaggagga aaaggcatca
     3361 gcatcgctga tgtgatgact gctggcgctc acggggtgcc gcgtgaagtg acagaaggcg
     3421 ttatcgacgg gcttaattat cccaatcatg aagcaattga tttttatcat cgctataaaa
     3481 cagatattca gttatttgcc gagatgggat tcaaatgctt tcgaacttcc attgcctgga
     3541 cacgaatctt tccgcaaggt gacgaacagg agccgaatga agagggttta caattttatg
     3601 atgatctgtt cgatgaatgc cttaagcagg gaatggaacc tgtggtgacg ctttcacatt
     3661 ttgagatgcc ttatcatctg gtgacaaaat atggtggctg gcgaaaccgt aaactgatcg
     3721 acttttttat ccgcttcgca tcaacggtct tcacgcgcta taaagaaaaa gtaaagtact
     3781 ggatgacgtt taacgaaatc aataatcagg tgaatttcag cgaaagcctg tgtccattta
     3841 ctaattccgg tatcttgtat tcgccagagg aagatatcaa tgagcgcgaa caaataatgt
     3901 accaggcggt acattacgag ttagttgcca gtgccctggc ggtacagact ggaaaatcga
     3961 tcaatcctga atttaacatt ggctgtatga tcgccatgtg tcccatctat cctctgacgt
     4021 gtgcacccaa cgatatgatg atggccacga aagcaatgca tcgtcgttac tggtttactg
     4081 atgttcatgc tcgtggatat tatccgcaac atatgctgaa ttactttgcc aggaaaggat
     4141 tcaacctcga tatcacacca gaagataacg cgattcttgc cagtggttgt gtcgacttta
     4201 tcggctttag ctactacatg tcttttacga cacaattttc gcccgataac ccgcaactgg
     4261 attatgttga accacgagat ttggtcagca acccttatat cgatacatcc gaatggggat
     4321 ggcaaattga tccggcaggg ctacgttatt cactcaactg gttctgggat catttccagt
     4381 tgccgctgtt tattgtcgaa aatggatttg gtgcggttga ccagagacaa gctgacggca
     4441 cggtgaacga tcactatcgc attgattact ttgcttccca tattcgggaa atgaaaaaag
     4501 ccgtagttga agatggtgtt gacttaattg gctacacccc gtggggctgc attgacctgg
     4561 tttctgccgg aacaggggaa atgaaaaagc gctacggaat gatttatgtc gataaagaca
     4621 acgaagggaa gggaacgctg gaacggatac gtaaagcgtc gttttactgg tatcgggatc
     4681 tcatcgccaa caatggcgaa aatatttgag acttgaggaa gatattacgg agatgtcagg
     4741 ctctgtgatc agagcctgac aataactaca tagcgtgttg attataatag ggcacgccga
     4801 gcgcatccga cttatcactg ccagtaccca tgtgatttaa atcaaacggt ggttgttcct
     4861 gcgatggggg aacaaccacg acattggcac aagaagtaac aactggctgc gtggtttgtt
     4921 ccgcaacgcc ctgcgcggaa aagagaataa gcgcagctgc tattgcggat gaaagtggtt
     4981 tcatgattgc ctcagtgacg ttgtcacgtt atcaactagc tgttgtaatg atttaatgga
     5041 tgtaaaaagc gcgattcgcc ctgcatattg gtcatccggt atttatgcgg cggaacatca
     5101 aagtaatttt tgaaggttcg ggtcagagtt tgttgcgact cgaagccata tcgttctgcc
     5161 agatagagta tcggctcgtt actttccttc agcttttgcg cgatttccgt catcttacgg
     5221 ctgcggatgt attggcctaa tgaatgaccg gtttcttttt taaacatccg ttgcaggtgc
     5281 catttagagt aacctgaacg ctctgacact ttctccagtg acaatggcga ttccaggttg
     5341 tcctcgatcc agtccaaaat gctatgaatg gtaatagcgt cagtattgcg tctggacatc
     5401 gtcatacctc ttttttgttt acggcaggac tttcttaagc aaatgctcaa gtgttgccac
     5461 ttcgtccgcc gtcaggtttt ttgttaattc ttgatgcagg tcctggccaa ctaattgatg
     5521 gcattgttcg catattgccg cgccgctggt ggtaagtttt accagtacgc cgcgcttatc
     5581 attcgggttc ggcaaccttt caacccagcc tttacagacc aggcgatcca gcatacgggt
     5641 cagtgctcca aggtcgacag acaacacttt tttcagttca accggagtaa tacacgccgc
     5701 gcagcggata gagcagagca ccttaaactg tgccgcggta atatccagcg gagacagata
     5761 ctcgttaagc agacgatctt tcttctgatt aaccatatgg attaagcgac ccaatggaat
     5821 aatttcattg aacagatcgc tggtactttt cacattagtt gccctggcaa gtaattagtt
     5881 gcaggggata atattgccca ggcaagtata agtcaaataa atgaattgac cgatgccacg
     5941 ttttgctaaa tcggttcaag ttttggtcaa tgcgatgcaa ggttagctaa agagtgacac
     6001 tttatccttg tttacccctt atacttttcg ctgataaccc agatacacag gataacaacc
     6061 accaatgtta gatttgttta aagcaattgg cctggggctg gtggtgttac tgccgttagc
     6121 taacccatta acaactgtcg cgttatttct tggcctggca ggcaacatga acagtgccga
     6181 acgtaatcgt cagtcgttga tggcctcggt gtacgtattt gccatcatga tggtggcgta
     6241 ttacgccggg caactggtga tggatacatt tggcatttcg attcccggtc tgcgaattgc
     6301 aggcggctta atcgtcgcgt ttatcggttt tcggatgctc tttccgcaac agaaagcgat
     6361 tgattcaccg gaggcgaaaa gcaagtcgga agagctggaa gatgaaccca gtgccaatat
     6421 tgcctttgtg ccgctagcaa tgccaagtac cgctggtccg ggaaccatag cgatgattat
     6481 tagctcggcg tccacagtgc gtcagagttc aaccttcgct gattgggtac tgatggttgc
     6541 gccgccgctg atctttttcc tggtggcggt tattttgtgg ggaagcctac gcagctccgg
     6601 cgcaattatg cggctggtgg gcaagggtgg cattgaagcg atctcccgtc tgatgggatt
     6661 cctgctggta tgtatgggtg tgcagtttat tattaacggc atcctggaaa tcattaaaac
     6721 gtaccattaa aaaaggcccg aatgggcctt tcaactattg cgtctgttct tcgagtgtca
     6781 ctggccagcg gcgaaatata atgattgacc aaattaacgc ggcaaaagca ggcaccgcgc
     6841 ccacataacc aatcatcgac attgaccagt gcaaactcac ctgattacct accaacgcac
     6901 ccgccccgat tccaatatta aatatcccgg agaatagcgc catcgcgacg tcggtagcat
     6961 ctggtgccag cgccagcact ttaacctgca taccaagccc gatgatcatc atcgcgatcc
     7021 cccagaaaat actcagcacc ccgaggtgta tttcactgtt cgccgcaggt aacagcaatg
     7081 ccaggcacac cagcaacagc gcaatcgccg tactcaccaa cgcagacgca tactgattac
     7141 ccagtttacc gaaaatcacg ctgccaataa tgcccgcacc accgagtaat aacagtaatg
     7201 ccgtggcaaa gttggcgctg aatcccgcaa tgttttgtac aaaaggctcg atatagctgt
     7261 atgccgtgta atgggcggtg acaaccacca cagttaacaa ataaatgctc atcaatgccg
     7321 ggcggcggaa caatagcggg aggcttttca gtgaaccgga atgctcactg ggcagtaagg
     7381 gaagtaactt aatcaggcac aaaagggtga taagcgcccc aataccaatc gcgaagaagg
     7441 tcattcgcca accgaaatac tggcccacaa tgcgcccgag aggtaaacct aagaccatcg
     7501 ccagtgctgt accggtggca attaaactca atgcctgtgc tcgcttcccg gccggagcca
     7561 tacggatcgc cagagacgcc gtaatcgacc agaaaatcgc atgtgcaaaa gccacaccaa
     7621 tgcgactgat caccagaacg gtaaagctcc acgataaaaa cgacagtacg tggctggcaa
     7681 taaacaccac aaacaggcag atcagtaatt tgcgccgttc aacctggctg gtcattaaca
     7741 taaaaggcaa tgacattagc gccactaccc atgcataaat ggtcaacata atgccgacct
     7801 gagcggtttg catgtgaaaa ctttgcgcaa tgtcagagag caggccaaca gggacaaatt
     7861 cggtggtgtt gaagatgaag gcggcgactg ccagcgtaac gacccgtagc cacgccactt
     7921 tgcgggaaac agtgtttgtt gtcataacag attatcgggt gagttgcgaa aaagtgatga
     7981 aatgatctta aaacggttaa tgatgtgaat acaatgcatt tgtgatctgg atcgcgtttt
     8041 ccgtttctct gacgctgatt ctgttccgcg tcagagagtc ggtaacatca cggtttaatg
     8101 acgttcaggg aggtctggcg ttttggcggt tgcgggtata ggtggtctga caggggagcg
     8161 aggcctgttt ttcaccaggt cttcgtgagt aacttttggc ggcgtttcat tttttacaaa
     8221 cagtttgagg gtcctgcgcg ccatatgttt gataatttta tcatcactta atgatgctgg
     8281 catcaacacg tcattcagat cactttcatt gggattcata aattggtgat agtgcaataa
     8341 catattggcg aaaaataaaa aaaagtcctg aaaatgagga gattcttgtt ctgctaaaag
     8401 ggataaaaag cagatgcaga cttcgggtag tacatgtttg ttagaaccta atgaatccat
     8461 caggctttgc aataaatcct cttttaattc gccgtctgtt atgtcgagaa tggcggtgat
     8521 caagttagga tctttattga cgaaccatct gtgaacctca gagttactcc ataatcgggg
     8581 gagtatgaca aaattaccgc gagagataaa attcaggaca agcaaatcaa actggcgtaa
     8641 gtaatctgca gcggtatggc gatccggagg caggggttca atatgtggtg tatgcgctct
     8701 tttgatggga acgccaaggt tgatcattgg aaattgttca ataaaatcat tcagaaacag
     8761 aaatagcaca cgctcgacgc cggtctgaaa tacgcgcccc atagtgacag gctttgggtt
     8821 gggagcgcaa aaatattaat ttctccatca tggcgaaaat tatctaaata aatagggctt
     8881 aaatttatta ctacctgaaa ttcaatattt tctttgtgtc ttccattctt gacgggataa
     8941 gtgagtttaa actgaacctt ttcattgtat agcgtgtcgt taccccgaaa agaagtcctc
     9001 tgaatatatg ataagttatc aaggttatta aatatctttt ttgtggctga gttgatgttt
     9061 aaagacgtgc gccactcttc atcacatatt attgtactta gcgcatcaat aaagtccttt
     9121 aaaacttctt ttacctgagg tgcaatatta cgtacgggaa aatcatcagg aagagatatg
     9181 taattactga tgcttactgg cgtaaccata tttgccccat atagtcataa gtcatcggaa
     9241 catcactata tagggcattt gagctgcaag aactatcaaa aatagctcat tggtaaccct
     9301 gttttgccga gtcaggcaca tccagtagtt gaataaatgc ctcaagcgga cgcgttttcg
     9361 caccgcgacg ccagaccagc caggttgtta accaacgcca ttgctcagct aacggccacg
     9421 cttcaacctg gtgatgcccc ggcatacttt ccagcataga gcgtggaata agcgcaatgc
     9481 ctgctcctgc gaccacacag gccaacatcc cgtgatacga ctccatctcg tgaatagttc
     9541 ccggagcggc accgtcagca tgaaaccagc tctcgaagtg gcgacgatac gaacaattgg
     9601 cgcggaaggc ataaatgtta ctgccgttaa cctgactggc gcggattact ggcgcatgac
     9661 cttgtggcgt gacgatcatc agctcctcgc ggtataccgg tatcccgtca atggcagtat
     9721 ggttaatggg cccatcaata aatgccgcat tcagttttcc ctccagcact ccttccagca
     9781 tagtgccgga agggccggtg gaaagtgaaa actgaatttt gggataacga tggttgaatt
     9841 ccgccagcgt ggccggaatg cgcactgctg cggtgctttc cagcgaacca agagaaaata
     9901 aaccttgcgg ctcatcgcca gcgacaacgc tccgcgcttc atccactaac gcgagaattt
     9961 gttggctata gcgtaaaaag ttatgcccgg caggagagag acgtaaacgc tgattctcgc
    10021 gaataaacag atctaccccc agttctgttt ccagctggcg tagacgggta gtgagattcg
    10081 acggtacgcg atgcactttt gctgcagcct gggtaatgct gccagcttcg gcaaccgcgt
    10141 tgaacatctc cagttgggtc agatccataa cattctcgat tcgtgaataa gtggcttaat
    10201 attattcatt ttaaagcaag agtaaatctg cgtatcttca taccatgact cataaaggag
    10261 ataccccgat gaccattact ccggcaactc atgcaatttc aataaatcct gccacgggtg
    10321 aacaactttc tgtgctgccg tgggctggcg ctaacgatat cgaaaacgca cttcagctgg
    10381 cggcagcagg ctttcgcgac tggcgcgaga caaatataga ttatcgtgct gaaaaactgc
    10441 gtggtatcgg taaggctctg cgcgcccgta gcgaagaaat ggcgcaaatg atcacccgtg
    10501 aaatgggcaa accaatcaat caggcgcgcg ctgaagtggc gaaatcggcg aatttgtgtg
    10561 actggtatgc agaacatggt ccggcaatgc tgaaggcgga acctacgctg gtggaaaatc
    10621 agcaggcagt tattgagtat cgaccgttgg ggacgattct ggcgattatg ccgtggaact
    10681 ttccgttatg gcaggtgatg cgtggcgcgg ttcccatcat tcttgcaggt aacggctact
    10741 tacttaaaca tgcgccgaat gtgatgggct gtgctcagct cattgcccag gtgtttaaag
    10801 atgcgggaat cccgcaaggc gtatatggct ggctgaatgc cgacaacgac ggtgtcagtc
    10861 aaatgattaa agattcgcgc attgctgctg tcacggtgac cggaagtgtt cgtgcgggag
    10921 cggctattgg cgcacaggct ggagcggcac tgaaaaaatg cgtactggaa ctgggcggtt
    10981 cggatccatt tattgtgctt aacgatgccg atctggaact ggcggttaaa gcggcggtag
    11041 ccggacgtta tcagaatacc ggacaggttt gtgcagcggc aaaacgcttt attatcgaag
    11101 agggaattgc ttctgcattt accgaacgtt ttgtggcagc tgcggcagcc ttgaaaatgg
    11161 gcgatccccg tgatgaagag aatgctctcg gaccaatggc tcgttttgat ttacgtgatg
    11221 agctgcatca tcaggtggag aaaaccctgg cgcagggtgc gcgtttgtta ctgggcgggg
    11281 aaaagatggc tggggcaggt aactactatc cgccaacggt tctggcgaat gttaccccag
    11341 aaatgaccgc gtttcgggaa gaaatgtttg gccctgttgc ggcaatcacc gttgcgaaag
    11401 atgcagaaca tgcgctggaa ctggctaatg atagtgagtt cggcctttca gcgaccattt
    11461 ttaccaccga cgaaacacag gccagacaga tggcggcacg tctggaatgc ggtggggtgt
    11521 ttatcaatgg ttattgtgcc agcgacgcgc gagtggcctt tggtggcgtg aaaaagagtg
    11581 gctttggtcg tgagctttcc catttcggct tacacgaatt ctgtaatatc cagacggtgt
    11641 ggaaagaccg gatctgacgc gacatattca gctctgatat actcgcaggt cttttcagac
    11701 ctgcggtcca ggagtagaaa gtggcagtcg ccatggataa tgcaatttta gaaaacatct
    11761 tgcggcaagt gcggccgctc attggtcagg gtaaagtcgc ggattatatt ccggcgctgg
    11821 ctacagtaga cggttcccga ttgggcattg ctatctgtac cgttgacgga cagctttttc
    11881 aggccggaga cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg ctgagtctcg
    11941 ttgtcgccat gcgtcattac tccgaagagg aaatctggca acgcgtcggc aaagatccgt
    12001 ctggatcacc gttcaattct ttagtgcaac tggaaatgga gcagggtatt ccgcgtaatc
    12061 cgtttattaa tgctggtgcg ctggtggttt gcgatatgtt gcaagggcga ttaagcgcac
    12121 cacggcaacg tatgctggaa gtcgtgcgcg gcttaagcgg tgtgtctgat atttcatacg
    12181 atacggtggt agcgcgttcc gaatttgaac attccgcgcg aaatgcggct atcgcctggc
    12241 tgatgaagtc gtttggcaat ttccatcatg atgtgacaac cgttctgcaa aactactttc
    12301 attactgcgc tctgaaaatg agctgtgtag agctggccag gacgtttgtc tttctggcta
    12361 atcaggggaa agctattcat attgatgaac ctgtggtgac gccaatgcag gcgcggcaaa
    12421 ttaacgcgct gatggcgacc agtggtatgt accagaacgc gggggagttt gcctggcggg
    12481 tggggctacc ggcgaaatct ggcgttggtg gcggtattgt ggcgattgtt ccgcatgaaa
    12541 tggccatcgc tgtctggagt ccggaactgg atgatgcagg taactcgctt gcgggtattg
    12601 ccgtccttga acaattgacg aaacagttag ggcgttcggt ttattaatgc agtctctcga
    12661 tccactcttc gcgcgtttat cccgttcaaa atttcgctct cgctttcgtc tgggcatgaa
    12721 agagcgtcag tattgcctgg agaaaggcgc gccagtcatc gaacaacatg cggcggattt
    12781 tgtcgctaaa cgtcttgctc cggcattacc ggctaatgac ggtaagcaaa cccccatgcg
    12841 cgggcatccg gtgtttatcg ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc
    12901 taagtggcat aacattccgc agggtgtttc gttaagcgaa gaacaacaac gttatatcgt
    12961 ggcggtaatc tatcactggc tggttgtaca gatgaatcag ccatgagaat tgcggaaaac
    13021 gataaaatag cattcccagg gtacctttat ttgccgccgc tgttagcgaa catgctatcc
    13081 tttatgagaa tttacttatt acgttgagat aattgccacc cgcaattgta tggacagata
    13141 attaatgcat gtacacccca tttcaacttt ccgtttgttc caggaaggtc atctgctacg
    13201 taatagcatc gctatttttg cgctaaccac gctgttctat tttattggtg cagagttacg
    13261 gctggttcac gaactttctc ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc
    13321 ccgctatgtc tggcttaatc gactgcatta ctatgcgatc agttatgtgg cgatgctggt
    13381 ttatgatgcc ataaccaccg aatggggact ggtttcactg gttatcaatt tctccaatat
    13441 gatgtttatt gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa
    13501 gtatgagcca gtaagtgcct tacggctatt taattactgt ctgattgccg cattattatg
    13561 cgctattgtc ggggcgattg gttcggtcag tattgatagt ctggatttct ggcctttgct
    13621 tgcggactgg ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt gtatgctgac
    13681 gttggctatt cctggagtgc tgccgcgctt taaagcagaa cagatgatgc ccgccatcgc
    13741 gcttattgtg tcggttattg cctcggtagt cattggcgga gcggggagtc tggcgttccc
    13801 gctgcctgca ttaatctggt gtgcagtgcg ctatacgccg caggtaacat gtctgttgac
    13861 ctttgtcacc ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg atatctcggt
    13921 cggttcgccg ttctccattc cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc
    13981 gatatgccca attatggttt cttttagcgt ggcagcgatc aattcgctaa tgaagcaagt
    14041 tgcgctgcga gccgactttg attttctgac tcaggtttac tcacggtccg gtctttatga
    14101 ggcgctgaaa agtccatcgc tgaaacagac acaacatctg acggtcatgc tgcttgatat
    14161 cgactatttc aaaagcatta acgataacta tggacatgaa tgtggcgata aagtgttaag
    14221 cgtgtttgcc cggcatattc agaagattgt cggtgataag gggctggtgg cgcgcatggg
    14281 cggcgaagaa tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc
    14341 ggaaaaaatc cgtaaaggcg ttgaactgca accattcacc tggcaacaaa aaacgcttta
    14401 tctcacggta agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga
    14461 ttttaataaa ttgatggtcg aagccgatac gtgtctgtat cgctcgaaga aagatgggcg
    14521 caaccgtacc agcactatgc gttacggtga ggaagttgtc tgacatcttt attcattccg
    14581 cgcgatttga atgcaatgtg ttctaccact ttatgatcca ggaaaaggcg tatttcctcg
    14641 attaaaccat gatccgcgcc acacttttct cataaaactg ctcattaatc atcaggagtt
    14701 gatcaaaaca ggggttcggc gtattaaagt ttgctaacct gtcgtaccaa ttcaaacagg
    14761 ttgtatgact aatcagaagg aaacccattg tgaaaacact aaatcgtcgc gattttcccg
    14821 gtgcacagta tccagaacgt atcattcagt ttggtgaagg taacttcctg cgcgcctttg
    14881 ttgactggca aatcgatctc ctgaatgagc acaccgatct gaattctggt gtggtcgttg
    14941 ttcgtccgat tgaaacttca ttcccaccgt cactgagcac gcaggatggt ctgtatacca
    15001 ccattatccg tggcctgaat gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct
    15061 cggttaatcg tgaaatcagc gtctacagtg aatacgatga attcctgaaa ctggctcaca
    15121 acccggaaat gcgctttgtt ttctctaata ccaccgaagc gggtatcagc taccatgcgg
    15181 gcgataaatt cgatgacgcg ccagcggtaa gttatccggc aaaactgacc cgtctgctgt
    15241 tcgaacgttt tagccatttc aacggtgcgc tggataaagg ttggatcatc atcccgtgtg
    15301 agttgatcga ctacaatggc gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt
    15361 gggcactgcc agaagcattt attcaatggc tggatcaggc taacagcttc tgttctacgc
    15421 tggtggaccg tatcgttacc ggttatccgc gcgatgaagt ggctaaactg gaagaagagc
    15481 tgggttatca cgatggtttt ctcgacaccg ctgaacactt ttacctgttt gtgattcagg
    15541 gaccgaaatc cttagcgact gaactgcgtc tggacaaata tccgctcaac gtgctgattg
    15601 ttgacgatat taaaccgtac aaagaacgca aagtggcaat cctcaacggt gcgcacaccg
    15661 cgctggtgcc ggtggcgttc caggcagggc tggataccgt aggtgaagcg atgaacgatg
    15721 ctgaaatttg cgcattcgtt gaaaaagcta tctacgaaga aattattccg gtactggatt
    15781 tgcctcgtga tgaactggaa tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt
    15841 acattaagca tcagctgctg tctatcgcgc tcaacggtat gaccaagttc cgcactcgca
    15901 tcctgccgca gctgctggca cggcagaagg caaaaggcac acttccggcg cgccttactt
    15961 tcgcattagc ggcattgatt gccttctatc gtggtgagcg taacggagaa acatatccgg
    16021 tacaggacga tgcacactgg ctggaacgtt atcagcaact ctggagccag catcgtgacc
    16081 gtgttatcgg tacccaggaa ctggtagcga ttgtgttggc agaaaaagac cactgggagc
    16141 aggacctgac gcaagtgcct ggtctggtcg agcaggttgc taatgacctg gatgcgattc
    16201 ttgaaaaagg gatgcgcgaa gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa
    16261 cccggtttcg accgggtttc ttatttcact atattagtta caacatccta tgtgccttta
    16321 tcattaacag atgccacatg cagcgcctgt taatgttgta agcttgtagg taatttgtaa
    16381 gtagcgtctt gagaatgtga tcgtaatcac gtttaatagg gcgacttttt cacgcctgaa
    16441 gataaccatg attgttcgtc cacaacaaca ctggctgcgc cgtatttttg tctggcacgg
    16501 ctcagtatta tccaagatat catcgcgctt actactcaat tttctctttt ctatcgctgt
    16561 tattttcatg ctgccctggt acacgcatct gggcatcaaa ttcaccctcg caccgttcag
    16621 cattctcggt gtcgccatcg ccatttttct tggttttcgt aataatgccg ggtacgcccg
    16681 ttacgttgaa gcgcgaaaac tttgggggca gttgatgatt gcctcacggt cgttactgcg
    16741 cgaggtaaaa acgacattgc cggattcggc aagtgtaagg gagtttgccc ggttgcaaat
    16801 cgccttcgcc cactgtttac gcatgacatt acgcaaacag ccacaggcgg aagtgctggc
    16861 tcattatctc aagactgaag atcttcagcc tgtactggct tcgaactctc cagctaaccg
    16921 tatcttgtta ataatggggg agtggttggc ggttcagcgc cgcaatgggc agctttcaga
    16981 tatccagttt attagcctca acgatcggct taatgatatt tcagcggttc tggcaggctg
    17041 tgagcgcatt gcctacacgc caattccctt tgcctacacc ctgattttgc atcgtaccgt
    17101 ttatctgttt tgtatcatgc tgccgttcgc gctggtcgtg gacctgcatt acatgacgcc
    17161 ttttatctct gtgctgattt cctacacttt tatttcgctg gattgtctgg cggaagaact
    17221 ggaagatccg ttcggtactg aaaacaatga tttaccgctg gatgccatct gcaacgctat
    17281 tgaaattgac ctgttacaga tgaacgatga agcagaaatt ccagcaaaaa ttcttcccga
    17341 tcgtcattac cagctgacgt gataattact ccgtacggcg ggcaacaata aacagacgcg
    17401 gaaatgccag cagtatctgt ccgttctctt gcagtggata ctgctcttcc agcatctgat
    17461 ggtagcgcgt aagaaaaagc tgctgttcgc tctcggtcag atcctgtaac cacggacgta
    17521 atccagtggc ggtcacccaa tcgataatcg cctggtgcga cggcatctgg tgatagtaag
    17581 tggttcgcca gatatcgacc tcacatccgg cttcgctcaa aatatcgtag taagcatgaa
    17641 cgccagccaa cggctcacgc ccgcgatccg ggtagttttg ttcccaggca acttcgcgca
    17701 tgagcacatg ggtcggctcc agccagttat ctggcatctg tactgccagc acgccctgcg
    17761 gattaagtaa agaaaccaga tgaggaaaca attcgtagtg gtcgggcagc cattgcagtg
    17821 aggcattagc aaaaatcaga tcgagtgcct gttccggttg ccgtttcgtt atggtgaacc
    17881 accgcctgta cggctttcat catcgccacc ggatggtcag actggaaaat attacgcccc
    17941 atatccacac cagaagcgcc ctgatcgata gcctgccagc acatttccag cgcctcgcgc
    18001 tccggtaatt ttttaccgcc agcaataaca atgggtaccg gacatccggc aacaatccgt
    18061 tcaaaacctt tttcgacata ataggttttg ataatttgcg cccccatttc agcggcgatt
    18121 cgagtcgcga gcgagaaata acgctgatcg cgcaccatat ctttgcccac gccagtcacg
    18181 gccatggtcg gcattcccac tttcattccg gcatcaacca gctgaataat atttttgatc
    18241 gactgatgtt catattcgct gccgatataa acctgcgccg ccaccgcgca actgttcagg
    18301 cgcacggcat catccatcga taacgccacg gcttcattac ttaattccgc cagaatagag
    18361 ttcgctcctg acgcccgcag taccaccggc ttattggtcg ctgggggaac tacgctgcgc
    18421 aaaatgccgc gcgtacacat taatacatcg gcatgttcaa acagcggggc gatatttata
    18481 tcaatgcgtt caagtccggt agtcggcccc tgaaaataac catggtcaaa ggccagcatc
    18541 acggttttac ccgttttcgg attaaatatc cgcgataagc gtgactgcat tccccaatcc
    18601 agcgcaccgc aacctttcag gataaaaggg atattttgtt gcgattgatc ggtacgaaaa
    18661 tctttaccat ctttaatatc gtctaaatct gccataactt actccggtta agtaatgcac
    18721 atcagaaatc gtatttgccg atattctctt tgttgaatat cacgcgctcc ggtaacagta
    18781 cgatgccatt accatccgct tcgtagtcat agccctgaac gctgtttggc gagacttcca
    18841 cctgacctac gccctggata tccagcttgt cgcccgtttt cattgatcct tttttcaata
    18901 atgcatccgc gacatacact gaaattttgc cttgctgaac cacatcccac aggccaaatt
    18961 ctttcaccgt gccgcgttcc acatatggac gcatcacgtt tggcgtactg aatccgacaa
    19021 tcgcaacttt gtcatttttc aggttttctg cggcttgtgc ggcagcgggc agggcgttgg
    19081 cgtcgggggc gataatggcg tcgagatcgc tatacgcttt taatattcct tctgcggttt
    19141 gtaatgattt agtggcatcg ttatagccaa actgcgtagt gacaatttcc cagccaggat
    19201 gctctttggc gattttcgct ttcgcttctt tcacccactg gttttggtcc gtaacggtgg
    19261 ggcttgagta gaaaaacgcg actttggctt tgtctttatt cacctgacgc gccgccatat
    19321 ccaccaacat acctcccaac tgggcgggcg ttccctgatt aatgtagtaa gagcggcact
    19381 ccggtttagt atcagagtcc caggtcagca ctctcacacc acgttgcatg gcgcgtttca
    19441 gtgccggaca caagccatca ggcgaaaccg cagaaacgat aatggcgtta taaccttgat
    19501 tgacgaagtt attaatcaac tgtacctgac cagaaacact gggttctgtc ggcccgtcgt
    19561 aggtcacatc aacgcccagc tctttacccg cttgttgtgc gccgttgcca ccgctggtaa
    19621 aaaatcccac gccaaccagt ttgggaataa atgcaatacg ctctgcggcc tgcacattca
    19681 tagagattgc ggcaatgcca agagcgctaa gtaaggcgat tttcttaaag cgatgaagtg
    19741 tcatgaagat atcctttatg gcaatgggtt attggcccga cgcgccagcc actctttaat
    19801 ttgctggcga tgcaggctaa cggaacgacc tacgacaacg acgataagta gcgcaccgga
    19861 aagggcgctg gacacctgat ttggcactcc tgccatttgc aaaccttgct gcaaatatcc
    19921 cactaataaa accgcaatgg cggtgccgat aatggaaccg gaaccaccat aaatattggc
    19981 cccgccaagc accacggcgg tgatggcggg catcagaaac gacgcaccga gatcggaacg
    20041 tgctgaacca aaatacgata ccagcagcac agcggcgacc gcagacgcca gccccgtcat
    20101 ggcatagagc gcacataagg tgcggttaac tggaatcgcg ctataaagcg ccacgcgcgg
    20161 gctttgccca atcaaaaaca cattacgtcc ggcatgggtt ttatgcagcc agagccagaa
    20221 aacgaggaga catatcagga agataatcag cggaacgggg agtcccagca catccaggtt
    20281 agcgaaatct gtaaacgcca tcgggaatcc accaatacct tcgtaccccg tcgctccggc
    20341 cataccggaa agcagcagag cgcttccggc aaacagatac agcgtgccaa gcgtaatcac
    20401 cagcgggtta actttggtat agataattaa tccggcgttg atcagcccgc acaatgcgcc
    20461 gagcagtaag gtcaggagta tcgccagcgg catcggcaca ccactttgaa acagtacgcc
    20521 caatgcaatg gcgcagaggc cgatggtcga accaaacgaa atatcgatcc cgccactgac
    20581 aatcaccatc gtcagcggta gggcgacaat gccaatgcag ataaagtcac tggcgctgaa
    20641 cagcaacata ttgagatcta acattcgcgg gttaattgca ccaaatgcga caatctcaat
    20701 aacgagcagt gcggcaagag ccagttccca accgtagcga atacgcatta tgcggcctcc
    20761 cgttttttac ctgacgaagc gggtttaacg gatggcggtg gcgtcataaa gcgggcatat
    20821 ttttgccgcc gtagattacg ttccagcgca caacgcaggc gtccatcaaa caccagcacc
    20881 gccagcagca cgaggcccgc gataaaatcg ttccaccatg ccggaatgcg caacagcacc
    20941 agtacgctat cgatctgcgt caggaaccat gcgccgagta ccgcaccgat gatcgcaccg
    21001 gaaccgccga gcaaactaat gccgcccagc acgcaggctg caattgcttt catctccagc
    21061 ccggtaccgg tctggttggg gataaaacca atctgcgaag caaacacaat tcccgccagt
    21121 gccgccatgc agccgttcaa cgaaaatgcc acaatgcgaa tggcttccgt acgaacaccc
    21181 agttgacgag cgccctgtaa attatcgccc gtggcataaa aactgcgtcc aaacgccgtc
    21241 tttgccagca gccaggccat aaatgccacc agaattatcg tcaaccaacc aatcgcggaa
    21301 acgccaaaca gcagcggtgc ggagagctgt ttcagttcgg cgggtaaccc ttcaatccat
    21361 ttaccgccag tccacagcaa catgatgcct ctgtacaacc ctaacgtgcc aagggtggca
    21421 acaatggcag ggatctttag ccacgcgacc aggacaccgt tgaaaaatcc cgcgagcaaa
    21481 ccaagcagta aagtcgcgac acaggcaaca ggtagtgaat atcctgcgtt cagtaacatc
    21541 cccaacagca ccgcgcacat tccggtaatc gaacccactg aaacatcaat attgcgcgta
    21601 agcattacca gtgtcgcgcc cattgccagc aggatcagga tttgcgcgct gctataaacc
    21661 atggtcagcg tttgcacact taaatactga cggtcgagaa aaccgggtaa tacaaacagc
    21721 agcaccaccg ccagcaatgc cgtgatttca cggttgttct gaataaactt cagcatgatg
    21781 cctcctgacg ctgactatcg ccgaaggcaa cgcgcataat agtctcgaca ttaatatcgc
    21841 gcccggtcag tgcagagtgg gtaatttcgc cctgatgcat cacatacaca cgatctgtca
    21901 tcagttcgat ctcttccaga tcagaggaga taagcagcac agccacattc tgtgcggaga
    21961 tgctgcgcaa cagctggtag atatcattac gggccgagac atccacgccg cgcgtcggct
    22021 catcgacaat caatgcttgc ggcgaagctt ccaggcattt ggcaatgagg attttttgct
    22081 ggttgccacc ggataatgtc cgtgcagctt gttccggttg gttaaattta atattcagcg
    22141 cccgacgata acgttccagg gtggcattat ctttcgcggt tttcgcccag aatccacgaa
    22201 ggttatgagt aagggcgcag acgttccagg ccagcgaagc atcgagattc agtccggatg
    22261 actggcgatc ttccggcaga taaaccagac cgcgcagtaa acgttctcca gtggataatt
    22321 tattgatctc tttaccattc agcataatgc gtccgctacg caaagtacgc agaccataga
    22381 acgtctcggc cagttctgtg cgtccggccc ccaccagccc agccaggccc agaatttctc
    22441 cggcattgag cgtcatgctg acattcctga aaccttcgcc ggtcagattt tccagtgtca
    22501 gcaccggcgt tccggcagca tgttgtgggc ggttaccagg taactccagc cataattttt
    22561 ggctggcaga gagcgatttt tcccgtacca ctggcgtgat ggcctgaata atgtcgtcgg
    22621 tagacagttc gctggttttg ccgcttaagg cgatggttcc gtcgcgcatc acgctaattc
    22681 gatcggcaat ctggcgaatt tccggcagct tatgcgagat aaaaacaata cccacgccag
    22741 tagcaagcag ctcttgcaag cgagtaaaca agcgttcggt ttccgcaggg gtaagcgagg
    22801 cggtaggttc atcgaggatc agaatccgcg agtcgcgcat tagcccgcgg aggatttcca
    22861 ccatttggcg atcggcgaca tccagcgatc ctgccagact atgcagatca aactggcagc
    22921 ccagcgccgc cagcaagttc ttcatttttt gcatggagag ctgttttttt gccatcccaa
    22981 acaggatgtt ttcttttatc gacaggcttg ggaaaagcag cggttcctgg ggaacgagat
    23041 aaatgcccag ctgatgagca tgaactggcg ttaatctggc gtagttgttg cccccaatct
    23101 ccagcgtacc gctatcagca ggggtaatac cggcaataat cttcattaac gtcgatttac
    23161 cggcaccatt gccgccgagc agggcgtgga cctccccctg atgcaacgta aaatcgatgc
    23221 ctttcaggac attgacccct gaatactgtt tataaaccga gcgggcgcaa agtagcggta
    23281 acgcgcgggt atcactcgtt tgcatatttc ccccgttcag ttttgcaggt gagttttgaa
    23341 caaatgtatt tctgctttta atttgttcat aaccttaggt ggacattgca catatttccg
    23401 acgaatagat cacaatttat gctattttga ttttcacggt tgcgtttgtt catgctcgta
    23461 gagtcaaact gtggctgcca tcacagatat aaatgcgcaa gaactgaaca attgcattaa
    23521 agatttaaat atgttcaaag tgaagaatga attatgacaa tcaacgattc ggcaatttca
    23581 gaacagggaa tgtgtgaaga agaacaggtc gcgcggatcg cgtggtttta ctatcacgac
    23641 gggctgaccc agagcgagat cagcgatcgt ctcggcctga cccgtttgaa agtgtcgcgc
    23701 ttgctggaga aagggcatca gtccggcatt attcgcgtac agattaattc tcgctttgaa
    23761 ggctgtctgg aatatgaaac tcaattacgt cgtcagtttt cgctgcaaca tgtccgggtg
    23821 atccctgggc ttgcggatgc tgatgtcggc gggcgactag ggataggcgc ggcgcatatg
    23881 ttgatgagtt tacttcaacc acaacagatg ctggcgattg gttttggcga ggcaaccatg
    23941 aatacgctgc aacgcttaag tggttttatt tcgtcacagc aaattcgcct ggtcacgctc
    24001 tccggtggcg tcggttctta tatgacgggt atcgggcagc ttaacgcggc gtgcagtgtg
    24061 aatattattc cggctccgtt gcgggcatcc tccgctgaca ttgcccgtac gctaaaaaat
    24121 gaaaattgcg tcaaagatgt tctgttagcc gcgcaagcag cggatgtggc gattgtcggc
    24181 attggggccg tcagtcaaca ggacgacgcg acgatcattc gctccggtta tatcagccag
    24241 ggcgaacagt taatgattgg tcgaaaaggg gcggttggcg acattttagg ctactttttt
    24301 gatgcaaaag gtgacgttgt cacggatatt aaaatacata acgaactgat tggcttacct
    24361 ttaagcgcgc tgaagaccat acccgtccgg gttggcgtgg cagggggaga aaataaagcc
    24421 gaagcaattg ccgctgcaat gaaaggcggt tatatcaacg cactggttac cgatcaggat
    24481 acagcagcgg cgattttacg tagttaaatt tgcatgacct tattaccggg aatgatggag
    24541 aacgtataga ggaacagaca gaacgcataa gccgaggata atctaatggc tcgactcttt
    24601 accccttcag aatcaaagta ttacctgttg gcgctggatg caggcaccgg aagtattcgg
    24661 gctgtgattt tcgacctgaa aggcaatcaa atagcagtgg gacaggcgga gtggcggcat
    24721 ctggcagtac cggacgttcc tggttctatg gaatttgatc tcaacaaaaa ctggcaactg
    24781 gcgtgtgagt gtatgcgcca ggcgctgcac aacgccggca tagacccgga gtatatcgct
    24841 gccgtttcgg catgttctat gcgtgaaggc attgttttat ataacaatga aggaacaccg
    24901 atctgggcct gcgccaatgt ggatgccaga gcagcgcgcg aagttagcga acttaaagaa
    24961 ctgcacaaca atacctttga aaacgaagtt tatcgcgcga ccggacaaac actggcttta
    25021 agtgccatcc ccagattact ttggctggcg caccatcgtt ccgatattta ccgtcaggca
    25081 tcaaccatca ccatgatcag cgactggctg gcctatatgc tcagcggcga actggcggtg
    25141 gatccctcta acgctggcac cacgggactt tgatctaacc acccgtgact ggaaacctgc
    25201 attgctggat atggctggcc tgcgtgccga tattctttct cctgtcaaag aaaccggcac
    25261 attgctgggc gtggtaagtt cacaagcggc ggaactctgc ggtctgaagg cgggcactcc
    25321 ggtggtcgtt ggaggaggcg acgtgcagct tggttgcctt gggttaggcg ttgtgcgtcc
    25381 ggcacaaacc gcggttcttg gcggcacatt ctggcagcaa gttgtaaatt tagccgcgcc
    25441 ggtgacagac ccagaaatga acgtgcgcgt taatcctcat gttattcctg gcatggtaca
    25501 agctgaatct ataagctttt ttaccggact caccatgcgc tggttccgcg atgctttctg
    25561 tgccgaagaa aaactgattg ccgaacgttt aggcatcgac acctatacgc tgctggaaga
    25621 gatggccagt cgggtgccgc ctgggtcgtg gggcgtaatg ccgatcttct ccgacagaat
    25681 gcgctttaaa acctggtatc acgctgcgcc ttcctttatt aacttgtcca ttgacccgga
    25741 taaatgtaac aaagcgacat tgttccgtgc gctggaagaa aatgcggcga ttgtatcagc
    25801 gtgtaacttg cagcaaattg ctgatttctc gaatattcat cccacctcgc tggtttttgc
    25861 tggcggaggt tcaaaaggga aattatggag tcaaattctc gctgatgtct cgggattacc
    25921 cgtcaatatt ccggtagtca aagaagccac cgcattagga tgtgccattg cagctggcgt
    25981 cggtgccgga attttttcat caatggcaga aaccggagaa cgcctggttc gctgggaacg
    26041 gacgcacaca ccagacccgg aaaagcatga actttatcag gattcacgcg ataagtggca
    26101 ggcagtttat caggatcagc tggggctggt tgatcatgga ctgacgacgt cgttatggaa
    26161 agcgcctggg ttatagtttc attgacggcc acatcatgat gaccgtggct gttagttttt
    26221 gagtatagtt tttcttattt cccttctttt agagctggct tgttaaatgc cagcttgcag
    26281 attcatcgtt cca
//
LOCUS       DAZKGS010000059        26234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKGS010000059 DAZKGS010000000
VERSION     DAZKGS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..815
                     /gene="adiY"
                     /locus_tag="ACHOQY_002361"
     CDS             54..815
                     /gene="adiY"
                     /locus_tag="ACHOQY_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9678445.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            952..2289
                     /gene="adiC"
                     /locus_tag="ACHOQY_002362"
     CDS             952..2289
                     /gene="adiC"
                     /locus_tag="ACHOQY_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9678446.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2393..4036
                     /gene="eptA"
                     /locus_tag="ACHOQY_002363"
     CDS             2393..4036
                     /gene="eptA"
                     /locus_tag="ACHOQY_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9678447.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4033..4701
                     /gene="basR"
                     /locus_tag="ACHOQY_002364"
     CDS             4033..4701
                     /gene="basR"
                     /locus_tag="ACHOQY_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9678448.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHOQY_002365"
     CDS             4702..5802
                     /gene="pmrB"
                     /locus_tag="ACHOQY_002365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9678449.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHOQY_002366"
     CDS             complement(5778..5867)
                     /gene="pmrR"
                     /locus_tag="ACHOQY_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9678450.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHOQY_002367"
     CDS             complement(5979..7481)
                     /gene="proP"
                     /locus_tag="ACHOQY_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9678451.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7745..8623)
                     /gene="rdcB"
                     /locus_tag="ACHOQY_002368"
     CDS             complement(7745..8623)
                     /gene="rdcB"
                     /locus_tag="ACHOQY_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP9678452.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8620..10848)
                     /gene="crfC"
                     /locus_tag="ACHOQY_002369"
     CDS             complement(8620..10848)
                     /gene="crfC"
                     /locus_tag="ACHOQY_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP9678453.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11251..11586
                     /gene="yjdM"
                     /locus_tag="ACHOQY_002370"
     CDS             11251..11586
                     /gene="yjdM"
                     /locus_tag="ACHOQY_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9678454.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11746..12189
                     /gene="yjdN"
                     /locus_tag="ACHOQY_002371"
     CDS             11746..12189
                     /gene="yjdN"
                     /locus_tag="ACHOQY_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9678455.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12322..13110
                     /gene="phnC"
                     /locus_tag="ACHOQY_002372"
     CDS             12322..13110
                     /gene="phnC"
                     /locus_tag="ACHOQY_002372"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9678456.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            13135..14151
                     /gene="phnD"
                     /locus_tag="ACHOQY_002373"
     CDS             13135..14151
                     /gene="phnD"
                     /locus_tag="ACHOQY_002373"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9678457.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14257..15036
                     /gene="phnE"
                     /locus_tag="ACHOQY_002374"
     CDS             14257..15036
                     /gene="phnE"
                     /locus_tag="ACHOQY_002374"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9678458.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15057..15782
                     /gene="phnF"
                     /locus_tag="ACHOQY_002375"
     CDS             15057..15782
                     /gene="phnF"
                     /locus_tag="ACHOQY_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9678459.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15783..16235
                     /gene="phnG"
                     /locus_tag="ACHOQY_002376"
     CDS             15783..16235
                     /gene="phnG"
                     /locus_tag="ACHOQY_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9678460.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16232..16816
                     /gene="phnH"
                     /locus_tag="ACHOQY_002377"
     CDS             16232..16816
                     /gene="phnH"
                     /locus_tag="ACHOQY_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9678461.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16816..17880
                     /gene="phnI"
                     /locus_tag="ACHOQY_002378"
     CDS             16816..17880
                     /gene="phnI"
                     /locus_tag="ACHOQY_002378"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9678462.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            17873..18718
                     /gene="phnJ"
                     /locus_tag="ACHOQY_002379"
     CDS             17873..18718
                     /gene="phnJ"
                     /locus_tag="ACHOQY_002379"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9678463.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            18715..19473
                     /gene="phnK"
                     /locus_tag="ACHOQY_002380"
     CDS             18715..19473
                     /gene="phnK"
                     /locus_tag="ACHOQY_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9678464.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19584..20264
                     /gene="phnL"
                     /locus_tag="ACHOQY_002381"
     CDS             19584..20264
                     /gene="phnL"
                     /locus_tag="ACHOQY_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9678465.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            20261..21397
                     /gene="phnM"
                     /locus_tag="ACHOQY_002382"
     CDS             20261..21397
                     /gene="phnM"
                     /locus_tag="ACHOQY_002382"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9678466.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            21397..21954
                     /gene="phnN"
                     /locus_tag="ACHOQY_002383"
     CDS             21397..21954
                     /gene="phnN"
                     /locus_tag="ACHOQY_002383"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9678467.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            21941..22375
                     /gene="phnO"
                     /locus_tag="ACHOQY_002384"
     CDS             21941..22375
                     /gene="phnO"
                     /locus_tag="ACHOQY_002384"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP9678468.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22377..23135
                     /gene="phnP"
                     /locus_tag="ACHOQY_002385"
     CDS             22377..23135
                     /gene="phnP"
                     /locus_tag="ACHOQY_002385"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9678469.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(23282..23611)
                     /gene="yjdP"
                     /locus_tag="ACHOQY_002386"
     CDS             complement(23282..23611)
                     /gene="yjdP"
                     /locus_tag="ACHOQY_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9678470.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23680..23997)
                     /locus_tag="ACHOQY_002387"
     CDS             complement(23680..23997)
                     /locus_tag="ACHOQY_002387"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP9678471.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            24250..>26234
                     /gene="yjcS"
                     /locus_tag="ACHOQY_002388"
     CDS             24250..>26234
                     /gene="yjcS"
                     /locus_tag="ACHOQY_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl/aryl-sulfatase"
                     /protein_id="HFP9678472.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6218 a         7353 c         6896 g         5767 t
ORIGIN      
        1 atcgctttgc tttagcgaga actggtcttt tatttgcact caggagtaca tgtatgagga
       61 tttgcagcga ccaaccttgt attgttttat tgactgaaaa agatgtctgg ataagggtga
      121 atgggaaaga acctattagc cttaaagcta accatatggc gttattaaat tgtgaaaata
      181 atattatcga cgtctcctct cttaacaaca ctttggttgc tcatattagt cacgacatca
      241 tcaaagatta cctccggttt ctgaataaag atctctcgca aataccagta tggcaacgta
      301 gcgctacgcc catactcacc ctgccatgcc tgacgccaga cgtctttcgc gttgccgcgc
      361 aacacagcat gatgcccgca gaaactgagt cagaaaagga acgaacacgc gcattattat
      421 tcacggtgct atcccgtttt ctcgacagta aaaaattcct ttcattaatg atgtatatgt
      481 tacgtaattg tgtaagtgac agcgtttatc aaattattga aagcgatatt cacaaagact
      541 ggaatcttag tatggtagcc agttgtttat gtcttagccc aagtctgtta aagaaaaagt
      601 tgaaaagcga aaacaccagt tatagccaaa taatcaccac ctgccgcatg cgttatgccg
      661 taaatgaatt aatgatggac ggtaaaaata tctctcaggt atcacagtcc tgcggctaca
      721 acagtacgtc gtactttatt tctgtcttta aagacttcta cggtatgacg ccgctgcatt
      781 atgttagtca gcacagagaa cgcactgtcg cctgattttt aaccttaacg aagagctata
      841 ttaataacgg catcagcgat aacccggtcg ataataattc aactatcgaa tgcaggcgta
      901 tgatatgacg taatttattg tcacgaagct cgccttcgca ggagtttaat tatgtcttcg
      961 gatgctgatg ctcacaaagt gggcttaatc cccgtcaccc tgatggtgtc ggggaatatt
     1021 atggggtcag gtgtttttct gttacctgca aacctggcct ctactggcgg gattgccatt
     1081 tatggatggt tggtgacgat tatcggtgcg ctggggctct cgatggtata cgccaaaatg
     1141 tcgttcctcg atccaagtcc tggtggttct tacgcgtacg cccgccgctg ctttggcccg
     1201 tttctcggtt atcaaaccaa cgtcctctac tggctggcct gctggatcgg caatatcgcc
     1261 atggtggtca ttggcgtagg atatttaagt tacttcttcc cgattctgaa agatccattg
     1321 gtattaacca tcacctgcgt cgtggtgctg tggatcttcg tcctgctgaa cattgtcggt
     1381 ccgaaaatga tcacccgtgt gcaggccgtt gccaccgtgc tggcgctgat ccccatcgtc
     1441 gggattgccg tatttggctg gttctggttc cgtggtgaaa cctatatggc ggcatggaac
     1501 gtcagcggcc tgggcacctt cggtgcaatt caaagtaccc ttaacgttac gctgtggtcg
     1561 ttcatcggtg tggaaagtgc ctccgttgcc gcaggtgtgg tgaaaaaccc gaaacgcaat
     1621 gtccctatcg ccaccattgg tggggtattg attgccgccg tttgctatgt actttctacc
     1681 accgcgatta tggggatgat ccctaatgcc gcactgcgcg tttctgcttc gccattcggt
     1741 gatgccgcac ggatggcgtt gggtgacacc gccggggcca ttgtttcctt ctgcgcagct
     1801 gcgggttgct taggttcact gggcggctgg acgttgctgg cgggtcaaac ggcgaaagcc
     1861 gctgccgatg acggactgtt cccaccgatt tttgcccgtg taaataaagc gggcacgcca
     1921 gtggcggggc tgattatcgt cggtattttg atgaccatct tccagctcag cagcatttca
     1981 ccaaacgcga ccaaagagtt cggtctggtt tcttccgtct cggtcatctt tacactggtg
     2041 ccatatcttt acacctgtgc ggcgttactg ctgctcggtc acggtcactt tggtaaagca
     2101 cgcccggcat atctggctgt tactaccatt gccttcctct actgcatttg ggccgtggtg
     2161 gggtccgggg cgaaagaggt tatgtggtca tttgtcaccc tgatggtaat caccgccatg
     2221 tatgcgctga attacaaccg gctgcataaa aacccgtatc ccttagatgt accaataagc
     2281 aaagattaat tccccttaat ccagcaaaca taaaagccaa ccttaagaac ttaaggttgg
     2341 cttaattttg ctttgcgagc atatgcgcac tttgttcgat ggaaacaccg tgatgttgaa
     2401 gcgcctacta aaaagaccct ctttgaattt actcgcctgg ctattgttgg ccgcttttta
     2461 tatctctatc tgcctgaata ttgccttttt taaacaggtg ttgcaggcgc tgccgctgga
     2521 ttcgctgcat aacgtactgg ttttcttgtc gatgccggtc gtcgctttca gcgtgattaa
     2581 tattgtcctg acactaagct ctttcttatg gcttaatcga ccactggcct gcctgtttat
     2641 tctggttggc gcggctgcac aatatttcat aatgacttac ggcatcgtca tcgaccgctc
     2701 gatgattgcc aatattattg ataccactcc ggcagaaagt tatgcgctga tgacaccgca
     2761 aatgttatta acgctgggat tcagcggcgt gcttgctgcg ctgattgcct gctggataaa
     2821 aatcaaacct gccacctcgc gtctgcgcag tgttcttttc cgtggagcca atattctggt
     2881 ttctgtacta ctgattttgc tggtcgccgc actgttttat aaagactacg cctcgttgtt
     2941 ccgcaataac aaagagctgg tgaaatcctt aagcccctct aacagcattg ttgccagctg
     3001 gtcatggtac tcccatcagc gactggcaaa tctaccgctg gtgcgaattg gtgaagacgc
     3061 ccaccgcaac ccgttaatgc agaacgaaaa acgtaaaaat ttgaccatcc tgattgtcgg
     3121 cgaaacctcg cgggcggaga acttctccct caacggctac ccgcgtgaaa ctaacccgcg
     3181 gctggcgaaa gataacgtgg tctatttccc taataccgca tcttgcggca cggcaacggc
     3241 agtttcagta ccgtgcatgt tctcggatat gccgcgtgag cattacaaag aagagctggc
     3301 acagcaccag gaaggcgtgc tggatatcat tcagcgagcg ggcatcaacg tgctgtggaa
     3361 tgacaacgat ggcggctgta aaggtgcctg cgaccgcgtg cctcaccaga acgtcaccgc
     3421 gctgaatctg cctggtcagt gcatcaacgg cgaatgctat gacgaagtgc tgttccacgg
     3481 gctggaagag tacatcaata acctgcaggg tgatggcgtg attgtcttac acaccatcgg
     3541 cagccacggt ccgacctatt acaaccgcta tccgccgcag ttcaggaaat ttaccccaac
     3601 ctgcgacacc aatgagatcc agacctgtag caaagagcaa ctggtgaaca cttacgacaa
     3661 cacgctggtt tacgtcgact atattgttga taaagcgatt aatctgctga aagaacatca
     3721 ggataaattt accaccagcc tggtttatct ttctgaccac ggtgaatcgt taggtgaaaa
     3781 tggcatctat ctgcacggtc tgccttatgc catcgccccg gatagccaaa aacaggtgcc
     3841 gatgctgctg tggctgtcgg aggattatca aaaacggtat caggttgacc agaactgcct
     3901 gcaaaaacag gcgcaaacgc aacactattc acaagacaat ttattctcca cgctattggg
     3961 attaactggc gttgagacga agtattacca ggctgcggat gatattctgc aaacttgcag
     4021 gagagtgagt gaatgaaaat tctgattgtt gaagacgata cgctgttatt gcagggattg
     4081 attctggcgg cgcaaactga aggctacgcg tgcgatggcg tgacaaccgc gcggatggcg
     4141 gaacaaagcc ttgaggccgg tcattacagc ctggtggtac tggatttagg gttacccgac
     4201 gaagatggac tgcattttct cgcccgtatc cggcagaaaa aatacaccct gccagtactg
     4261 atcctcaccg ctcgcgatac gctgaccgac aaaatcgctg ggctggatgt cggtgccgat
     4321 gactatctgg tgaagccttt tgcgctggaa gagttacacg cccgtatccg cgccctgcta
     4381 cgacgccata ataatcaggg cgaaagtgag ctgattgttg gcaatctgac gctgaatatg
     4441 ggtcgccgtc aggtatggat gggcggtgaa gagttgattc tgacgcccaa agaatatgct
     4501 ctgctgtcac ggttaatgct caaagcaggc agtccggtgc atcgggaaat tctctacaac
     4561 gacatctata actgggacaa tgaaccctcg accaacaccc tggaagtgca tatccacaat
     4621 ctgcgcgaca aagtgggcaa agcccgtatc cgcaccgtgc gcggctttgg ctatatgctg
     4681 gtcgcgaatg aggaaaacta attgaatctg atgcattttc tgcgccgacc aatatcgctg
     4741 cgccaacggc tgatattgac catcggggcc attttgttgg tgtttgagct gatcagcgtc
     4801 ttctggctat ggcatgaaag taccgagcag attcagctgt ttgagcaggc gctacgcgac
     4861 aatcgcaaca acgatcgcca catcatgcgt gagatccgcg aggcggtcgc cagcctgatt
     4921 gtccccggcg tctttatggt cagcctgacg ctatttatct gctatcaggc ggtgcgccgc
     4981 atcacccgcc cgctggcgga gctgcaaaaa gagctggaag cgcgcactgc cgacaactta
     5041 acgcccattg ccattcacag cgccaccctc gaaatcgaag cggtggtttc ggcgttaaac
     5101 gatctggtca gtcgcctgac cagcacgctg gataacgaaa ggttgtttac cgctgacgtc
     5161 gcgcacgaac tgcgaacgcc actggcgggg gtgcgtttgc atctggaact gctggcgaaa
     5221 acgcatcaca ttgatgtagc accgttagtg gcacgacttg atcagatgat ggagagcgtc
     5281 tcccagctgc tgcaactggc gcgtgccgga cagtcatttt cttccggtaa ttatcaacat
     5341 gtaaaactgc tggaagatgt cattctcccc tcgtatgacg aactcagtac catgctcgac
     5401 cagcgacagc aaaccctgct attgccagag agcgccgccg acatcaccgt tcagggtgat
     5461 gcaaccctgc tgcggatgtt attgcgaaac ctggtagaaa acgcccatcg ttacagcccg
     5521 caaggcagca acattatgat taagctgcaa gaagatggcg gagcggtcat ggcagttgaa
     5581 gatgaaggac caggtattga tgagagtaaa tgcggggagt tgagtaaagc gtttgtacgt
     5641 atggacagcc gttatggcgg gattggtctg gggttaagta ttgtcagccg cattacacag
     5701 ttgcatcacg ggcagttttt cctgcaaaac cggcaagaga cttccggcac gcgggcctgg
     5761 gtacggctga agaaagatca gaacgtggca aaccagatat aaagaaagct gctgaccacc
     5821 agcacgctga acacggtagt taaactttca taaacacggt ttttcattgc actctcctcc
     5881 gttaacctgg aggagagtat gcgcgtcaga gattaagtga accttaagag ttcaattggc
     5941 ctgataagac agcgtcacat caggccatcc gtttcagctt attcatcaat tcgcggatgt
     6001 tgctgcacca ggcgggtacg tttcgcctgc aaatcggcaa tctcgtggtc aatattatcg
     6061 attttctgct cgatattatc gtaatgctcg acgagaattt ccttcgcttc ctgtatatct
     6121 gacgccgccg gtgtcgcacc tttcaacgga cgatttgccg tctctttcat ggttacgccg
     6181 gtgattaaac caatcaccgc cactaccatc aggtaatagg caggcatcat cagattctgc
     6241 gagctttcga ccagccaggc cgccagtgtt ggcgtcagac cggcaaccag caccgaaata
     6301 ttaaatgccg ccgccagcgc gctgtaacgg atatgcgtcg ggaacatcgc tggcaaggta
     6361 gaagccataa cgcccgtaaa gcagttaagg atcaccgcca gcatcagtaa cccggcaaaa
     6421 atcaggccga tgacgttact gttaatcaga ataaacgccg ggatcgccaa cacaaacagg
     6481 gcaacactac caagtagcac aaacggacga cggccaaaac ggtcactcag caagcccatc
     6541 accggctgga caaacagcat accgatcata atggcgataa taatcagcac cccgtggtct
     6601 tcggagtaat gcaggttatg cgacaaataa ctcggcatat aggtcagcaa catgtagtaa
     6661 gtcacgttgg tggcaattac cagaccaata catgtcaaca ggctgcgcca gtgtttggtg
     6721 gcaatctctt taaacgagac tttcgggcca tcctgcaaac cttcgcggtc gccctgttcc
     6781 agtttatcga catgctgctg gaacgccgga gtttcttcca gcgcatggcg caggtaaagc
     6841 ccgataatcc ctaacggcag agcaataaag aacggaatac gccagcccca gtcgaggaag
     6901 ttcgcttcgc cgacaatggt cgaaattaac accactacgc cagcacccag cacaaacccg
     6961 gcaatagaac cgaagtccag ccagctgccc ataaagccac gtttacggtc aggtgagtat
     7021 tccgcaacaa atatcgacgc cccggtatat tcaccgccga ccgagaaacc ttgtgccatc
     7081 ttacagatca acagcagaat cggtgcccaa ataccaatcg tgtcgtagga cggtattaag
     7141 ccaatacaga acgtactgat cgacataatc acaatagtga tagcgaggat cttctggcga
     7201 ccatatttat cgcccaacat accaaagaag agcccgccaa gcggtcgaat cagaaaggga
     7261 acggagaaag tggcaagtgc agcaaccatc tgcacgctgg ggtcagcccc cgggaaaaaa
     7321 actttaccta atgcgtaagc aacaaaacca taaacaccaa aatcaaacca ttccattgca
     7381 ttacccagtg atgctgcggt aatggcttta cgcagtttac cgtcatcaat aatggtgaca
     7441 tcacgaaggg taattggttt tacttttttc cttttcagca tagctttcct cgcagagttg
     7501 gcccatttcc gcaaacgcca gaaaaatcag cgcgacggcc gcaaaagggg agcgggaatt
     7561 gcaggatgca atctctgtaa caagcgtagc gggtttgctt acaccctccg gttaaggaat
     7621 gtacaatcaa accagttgac gcctgaattg ggcagttaat gaccttttta ccctaccagt
     7681 gtttaaattt gtgatcaact tcacacatgg atttcgttta ggactcattg atgtaactga
     7741 ttatttacgc caaatttaca gagaagattt cctcgagcat tgcccccgcc agacgctgcg
     7801 ctgaacagag tcgtggcatc cccagcgcga cagtttgcca gcgttgggtc actgaccagc
     7861 aaacgggttg tttatctgcc gtggtccact ttcccagcca acttaaaaaa tcagcatgat
     7921 ccccgcccgt cggaacggtc gaagaggcaa accactgatg gtaatagtga cgcgtggtcg
     7981 gggccgcatt gatactctct gccagatgtt gtgtcgcttt cacgcgcaaa tcacccagca
     8041 acaacgccac cgcttgcggg cttcccagac gacaggcatc gccaaatgcc ccccaacgca
     8101 actcttcgag aacgataaaa cagcgcccag gcaacgacca ggcgttatac gccccagcac
     8161 gccagcgggt aaaaatttgc tcgctctgat cacctgcctg caccagtagg cctcgttgcg
     8221 ttaacgcctt ttccttccag cggcaacggg ctgaaaacca ctcatacaag gcggcaattt
     8281 gctgggggag ctccgggaaa gcagagtgct ggcggtgatc cattttttgc agcagcgcca
     8341 gatgctgcgc gatctgcgtc agtgccagat gaccgggatc cagcatcgac gccagttttt
     8401 ccaccaatgt cagacgcagg ctaagcggcg catttaacat gccgccgttc gtccatgtct
     8461 gaagctgcac gcgcgcaaga agttcctgca tcagtcgttc acgaaactgc tgctgatggg
     8521 cttgtggaag gcgggcatca tcgccctgcg ccagatccac taaaaagcgg ggataaaccg
     8581 actccagcac gcgaccgggg ccgtcaagta acgtcttggt caatatcgtt ctgccgtgaa
     8641 aagtgtttga atatcatcgc gtaacagctg ggcgtcggtg taaatccagc cgtgagtcat
     8701 cacagtccgc tgcaattgct gctgcatcag cctgaccacc gattcatttt gttgacgcag
     8761 agccagactt tcgcgtaaac gcgtctgtaa ttccgtcaaa catgaagcga actcagcgaa
     8821 aaaagtattc atgcctgccg taacagattc atcgacctgc tctgccagaa ctttagccat
     8881 ttgttggcaa taaagatcga cttctgcgct taaagctcgt tgcaacacac tgtaatcaac
     8941 cgtctctgtc ggggatttct catttccccg tccccagtcg ggctgattca accagcgcga
     9001 aaaagtctca cgcacaacgc ctaaacgcgt gctctgctcg tccgttgctt cctggcgcga
     9061 aatgacttca ctgaacagct ggcgagtgtt gaagtgggga actgcgccgt gaaaaacagg
     9121 aaaatgaaac ccaggacgaa accctgactc gctcaattcc attttgactt gttgctcaat
     9181 ggggcgaata acatcggtta acacccggca aagggtggat tccagctcgg caaaacgcag
     9241 cgtaaagtcg cgactgatgg tgttctgcgc cgtctgtaac agtgtctcac agcgggtacg
     9301 aatctcgctt aacggctccg aatcatcctg aaacaacgcg gctaactgtg cattcaccgc
     9361 atcttgctgt tgacgcagaa agtggttggc ggaggccaag gccagctcga tttcatgttt
     9421 aatctcgccg ctcacctgcg cctgattgag ttgcaatagc tgcaaacttt cttcgacctg
     9481 atggatattt tgccgcaatt gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag
     9541 gtattcccgc gcctgctgcg cgtaattcaa cagtttatgc gcggcagatc gcagagcata
     9601 caacgaggcg ttagcgtaag cggcatgaag caacgcctga attggctggg cgaacagcga
     9661 atcttcccac aactgatcgg cagcatgacg aatatgttcg aggtccgcca gatcggcatg
     9721 acgccagcgc ctgccgagcg cggcatgggc aaaatcttcc acccagcgtt gttgctctgg
     9781 cggtggtaac ttaccgttgt tggctaactc atggcgcgcc cgattcgcca ggtagcccca
     9841 catcgacgac accggaaata tctgctgtgg cgtaatacag cctttcatca gcgtcccgga
     9901 aatcagtgcc cgcacctggt cggcgtcgtc actgttacga tcctgttgat cgaacttatt
     9961 gaccagcaca tacagcggca ccgattgccc caccgccaaa atcgcctcac ggacctcttc
    10021 atcggagatc gatttcagtt gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc
    10081 cagctgctgg ttaagcattt tttgcagatg cggttgcccg gcttcatttg gcccgggggt
    10141 atccagtaac gtcaactgac cgggataact ttccagcccc gccagatgga caaactccac
    10201 ttcaatcacg ggaatatgct caatggcggc gtaagcagaa aaaggaaaat cgacgtccag
    10261 cgccttcgcc agtcgcacta aatcattcaa acttttcaga caatgaaaaa taggctgggc
    10321 acccagataa tatttttcga aagcgacgcc attttcgatc cgctgcataa gcgcacgcat
    10381 atctttatct atttccagca catcggtcag atgcttaata tcgcaatcac gcaggcgctg
    10441 ttgtaatttt tgaattaaac ggtcgattgg cgcgacatgt gaaaaatgca gtaccggttc
    10501 tttttgcccg ggcgtatggc gaataagcgt cggcagcgca gtcattgggc gattacgatt
    10561 aggcaaaacc tccgtaccaa caatggcatt aatggtggtt gatttccctg ctttcatggt
    10621 accgacaatc gccagcacca tttccagtcg ggaaatttta cgcaactcat tattcagcat
    10681 cgcgtgacgt tcggcgatat taggctgact ccagggtaaa gccagttgtg gcgcatcgtc
    10741 tccgggtaca gagagaggca ttttttccag taactgcaac tgttggcgag aaagctgtaa
    10801 caggcgttca gcctcctgac ttaactcata cagggtctgt gtgtacatag aaaattcttc
    10861 cttaaagcaa attttgttac ttttatttag ccagattgtt tttgagttct gttttcggct
    10921 tttataatta ctgcaagaaa taattttata tttagcgtgt tgttttttat tagaataaat
    10981 aacgtcttcc gatacgttta aaacatcaga aacataaaaa tatcatgtga attaaaaaaa
    11041 agaacaagta gagcattaac attatcttaa ataataaatg gaggcaaaaa gattattttc
    11101 tgtttgcgtt tcctttcaaa tgaaaacgat cgtcgtctaa aatcagcagt acccccgaca
    11161 aactcaggga ttttgtgtat aattgcggcc tttttcggca atctgccgtt ttttggcgct
    11221 tttgccctgc tgacttttga ggaaatccac atgtcattac cacactgccc aaaatgcaac
    11281 tccgaataca cttacgaaga taacggcatg tacatctgcc cggaatgtgc ctacgaatgg
    11341 aacgacgcag aacctgcaca ggaaagcgac gagctgatcg ttaaagatgc taacggcaat
    11401 ctgctggctg acggcgacag cgttaccatc attaaagatc tgaaggtgaa aggtagctct
    11461 tcgatgctga aaattggcac caaagtgaaa aacatccgcc tggttgaagg cgaccataac
    11521 atcgattgca aaatcgacgg ttttggtccg atgaaactga aatctgagtt tgtgaaaaag
    11581 aactgattgt attgtgatcg gtaagccgga taaggcgctc gcgccgcatc cggcaacgat
    11641 gccgactgcc tgatgcgacg ctgaccgcgt cttatcaggc ttccctctca tctgtataaa
    11701 tttcgaacta cacttaactg gcttctctta actgaggtca ccatcatgtc gttaagtccc
    11761 tacctctctt ttgccggtaa ctgtgccgac gcaattgctt attatcaacg tacgttgggc
    11821 gctgaactgc tctataaaat cagcttcggc gaaatgccaa aatcagcgca ggacagcgcc
    11881 gagaactgcc cttccggaat gcaatttccc gataccgcca tcgcccacgc caacgtgcgc
    11941 attgccggaa gcgacatcat gatgagcgat gctataccgt caggaaaagc cagctactcc
    12001 ggctttacgc tggtgctcga ttcgcaacag gtcgaagaag gaaaacgctg gtttgacaat
    12061 cttgccgcta acggaaaaat cgaaatggcc tggcaggaaa ctttctgggc gcatggcttt
    12121 ggcaaagtca ccgataaatt tggcgtaccg tggatgatta atgtcgtcaa acaacaacca
    12181 acgcaataac ccgccgggag gcccgccctc ccgcactgtc atcgaattcc cgttaactct
    12241 tcatctgtta gtcactttta attaaccaaa tcgtcacaat aatccgccac gatggagcca
    12301 cttttttagg gaggctgcat catgcaaacg attatccgtg tcgagaagct cgccaaaacc
    12361 ttcaatcagc atcaggcgct gcatgcggtt gatctgaaca ttcatcacgg tgaaatggtg
    12421 gctctgcttg ggccgtcggg ttccggcaaa tccacccttt tacgtcactt aagcggtttg
    12481 attaccggcg ataaatccgc cggcagccat atcgagctgc tgggccgcac agtccagcgc
    12541 gaaggccgtc tggcgcgcga tatccgcaaa agccgcgcca acaccggcta catcttccaa
    12601 caattcaacc tggtgaaccg cctgagcgta ctggagaacg tgctgattgg cgcgctcggc
    12661 agcacgccgt tctggcgcac ctgttttagc tggttcaccg gcgagcagaa acagcgcgcg
    12721 ttacaggcgc tgacccgcgt tggcatggtg cattttgccc atcagcgcgt ttccaccctc
    12781 tccggcggac agcagcagcg tgtggcgatt gcccgcgcgc tgatgcagca ggcgaaggtg
    12841 attctggccg atgaacccat cgcctcgctg gacccggaat ccgcccgcat cgtgatggac
    12901 accctgcgcg acatcaatca gaacgacggc atcaccgtgg tcgtcacgct gcatcaggtg
    12961 gattacgccc tgcgctactg cgaacgcatc gtcgccctgc gccaggggca cgttttctac
    13021 gacggcagca gccaacagtt tgataacgaa cgttttgacc atctctaccg cagcattaat
    13081 cgcatcgaag agaacgcgaa agctgcctga catccccatc attgaggaaa acgaatgaac
    13141 gctaagataa ttgcctcgct ggccttcacc agcatgttca gcctcagcac cctgttaagc
    13201 ccggcacacg ccgaagagca ggaaaaggcg ctgaatttcg gcattatttc aacggaatca
    13261 cagcaaaacc tgaaaccgca atggacgcca ttcttacagg atatggagaa gaagctgggc
    13321 gtgaaggtga acgccttctt tgccccagac tacgcaggca ttatccaggg aatgcgcttc
    13381 aataaagtgg atatcgcctg gtacggcaac ctgtcggcaa tggaagcggt ggatcgcgcc
    13441 aacggccagg tcttcgccca gacggtcgcg gcggatggat cgccaggtta ctggagcgtg
    13501 ttgatcgtca acaaagatag tccgatcaac aacctgaacg atctgctggc gaagcggaaa
    13561 gatctcacct tcggcaatgg cgatcctaac tccacctctg gcttcctcgt ccccggttac
    13621 tacgtctttg ccaaaaacaa tatctccgcc agcgacttta agcgcaccgt caacgctggg
    13681 catgaaacca acgcgctggc cgtcgccaac aagcaggtgg atgttgccac caacaacacc
    13741 gaaaacctcg acaagctgaa aacctccgcg ccagagaagc tgaaagaact gaaggtgatc
    13801 tggaagtcgc cgctgatccc aggcgatccg atcgtctggc gtaaaaacct ttccgaaacc
    13861 accaaagaca agatctacga cttctttatg aactacggca aaacgccgga agaaaaagcg
    13921 gtgctggaac gcctgggctg ggcgccgttc cgcgcttcca gcgacctgca actggtgccg
    13981 attcgccagc tcgcgctgtt taaagagatg cagggcgtga aaagcaataa aggactgaat
    14041 gagcaggaca agctggcaaa aaccaccgag attcaggcgc agctggatga cctggaccgc
    14101 ctgaacaacg cgttaagcgc gatgagttcg gtgagtaaag cggtgcagta aatcgtaggt
    14161 cggataagac gccccggcgt cgcatccgac aatgtgcagg cgttgatgcc ggatgcggtg
    14221 caagcacctt atccggccta cagaccggag ccaaacatgc aaaccatcac catcgcccca
    14281 cccaagcgca gctggttctc gcttctgagc tgggccgttg ttctcgccgt gttggtcgtc
    14341 tcgtggcagg gcgcggaaat ggccccgctc acgctgatta aagacggcgg caacatggcg
    14401 acgttcgccg ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa
    14461 atggccgtca cgctgcaaat cgccgtctgg ggcacggcgc tggcggtggt tctctccatc
    14521 cccttcggcc tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc
    14581 cgcctgatgg acgcctgccg cgtcattaac gaaatggtct tcgccatgct gttcgtggtc
    14641 gccgtcggcc tcggcccgtt cgctggcgtg ctggcgctgt ttatccacac caccggcgtg
    14701 ctctccaaac tgctttccga agcggtggaa gcgattgaac ccggcccggt ggaaggcatt
    14761 cgcgccaccg gcgccaacaa gctcgaagag atcctctacg gcgtgctgcc gcaggtgatg
    14821 ccgctgctga tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc
    14881 gtcggcatgg tcggtgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc
    14941 cagttccaac aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat
    15001 ttcctctctc aacggttgcg taagcacttt atctgataag cgaggcattg atatctatgc
    15061 acttgtctac acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg
    15121 agcaggagct tcgtcaacac taccgctgcg gcgactatct tcccgccgag cagcaactgg
    15181 cggcgcgctt tgaggtgaac cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa
    15241 aaggctgggt acagcgccgt cagggcgtcg gcgtgctggt gctgatgcgc ccgttcgatt
    15301 acccgctcaa cgcccaggcg cgttttagcc agaacctgct ggaccagggt agccatccca
    15361 ccagcgaaaa actgctgtcg gtattgcgcc ccgcgtccgg tcacgtcgct gacgcgctgg
    15421 ggattaccga gggcgagaac gttatccatt tgcgcaccct gcgtcgcgtc aacggcgtcg
    15481 cgctctgttt aatcgaccac tactttgcgg acctcaccct ttggccgacg ctgcaacgct
    15541 tcgacagcgg ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca
    15601 gccagacgcg gatcagcgcc cgtcgcgccc aggccaaaga gtgccagcgt cttgaaattc
    15661 cgaatatgtc gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc
    15721 cggcggagta ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact
    15781 gaatgcacgc agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac
    15841 cggctgaact ggcagcccgc ctgaaagcac tgaacatcac cgccgactat gaggttatcc
    15901 gcgccgccga aaccggcctg gtgcagattc aggcgcggat gggcggcacc ggcgaacgct
    15961 tttttgccgg cgacgccacg ctcacccgcg ccgccgtacg cctgactgac ggcacgctcg
    16021 gctacagctg ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg ctgattgacg
    16081 cgctgatgca gcaatctcgc tattttcaaa acttatcaga aacccttatt gccccgctgg
    16141 acgctgaccg tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg
    16201 acttctttac gatggttcgc ggagacaacg catgaccctg gaaaccgctt ttatgcttcc
    16261 cgtgcaggat gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt
    16321 gattgtcgcc ctgcatcagc tcaagcgcgg ctggcagccg ctgaatatcg ccaccaccag
    16381 cgtgctgctg acgctggccg ataacgacac gccggtgtgg cttgccgcgc cattgagtaa
    16441 cgatatcgtc agccagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga
    16501 acaggcgacc ttcgcggtga cggatgaggc gatttccagc gaacagctta acgccctttc
    16561 caccggcacc gccgttgcgc cggaagcggg tgcaacgctg attttacagg tcgccagcct
    16621 gagcggcggg cgcatgttac gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc
    16681 tccgcagctg ccggagtgca ttctgcacga actcaccgag cgcccgcacc cgttcccgct
    16741 cggcatcgac ctgatcctga cctgcggcga gcgcctgctg gctattccgc gaaccactca
    16801 tgtggaggtg tgctgatgta cgttgccgtg aaagggggcg agaaggcgat cgacgccgct
    16861 cacgccctgc aagagagccg acgccggggc gataccgatt tgcctgaact gagcgtcgcc
    16921 cagattgaac agcagcttaa tcttgcggtg gatcgcgtga tgaccgaagg cggcattgcc
    16981 gaccgcgaac tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcaatt
    17041 ttcctgctgc gcgcctaccg caccacgctg gcgaagctcg cggtaagcga gccgctcgac
    17101 accacagaga tgcgtctcga acggcgtatt tcggcggttt ataaagacat tcccggcggc
    17161 cagctgcttg gcccaaccta cgactacacc catcgcctgc tcgatttcac cctgctggca
    17221 aacggtgaag cgccgacgct gaccaccgcc gacagcgaac aacagccgtc gccgcacgtt
    17281 ttcagcctgc tggcgcgtca ggggctggtg aagtttgaag aagatagcgg cgcacagccg
    17341 gatgacatca cccgcacgcc gccggtttac ccctgctcac gctcctcccg cctgcaacaa
    17401 ttaatgcgcg gggacgaagg ctatttgctg gcgctggcct actccaccca gcgtggttac
    17461 gggcgcaatc acccgttcgc gggcgagatc cgcagtggtt acatcgacgt gtcgattgtg
    17521 ccggaagagc tgggatttgc ggtaaacgtc ggcgaactac tgatgaccga gtgtgaaatg
    17581 gtcaacggtt ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg
    17641 gtattcggca tgagcgagcg caaagcgatg gcaatggcgc tggtcgatcg tgcgttgcag
    17701 gctccggaat acggcgagca cgcgacaggc ccggcgcagg atgaagagtt tgtgctggca
    17761 catgccgaca acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc ccactacgtc
    17821 gatttccagg ccgaactgga gctactcaaa cgtctgcaac aggagaagaa ccatggctaa
    17881 tctgagcggc tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc
    17941 tatcttaaaa gcggtggcga tccccggtta tcaggtgccg tttggcgggc gcgagatgcc
    18001 gatgccatac ggctggggaa ccggcggcat acagctcacc gccagcgtga ttggcgaaag
    18061 cgacgtgctg aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg
    18121 caacttcttt aagcgcgtga ccggggtaaa caccactgaa cgtacggacg atgcgacgct
    18181 tatccagacg cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt
    18241 ccaggtgcca atcccggaac cgctgcgctt tatcgagccg cgcgaaacgg aaacccgcac
    18301 catgcacgcg ctggaagagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg
    18361 cttcggtcat atcgccacta cctatgccta tccggtgaag gtgaacgggc gctacgtaat
    18421 ggacccgtcg ccgatcccga aattcgataa cccaaaaatg gacatgatgc ccgccctgca
    18481 actgttcggc gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga
    18541 aagtctcgat ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat
    18601 ctgcggatcg acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat
    18661 gtttgtctgc tccgataccg attattgccg ccaacagagc gaggcaaaaa accaatgaat
    18721 caaccgttac tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc
    18781 gatgtctctt ttgatttatg gccgggggaa gtgctgggca ttgtcgggga gtccggctcc
    18841 gggaagacca cgctgctgaa atcaatctcc gcgcgcctga cgccgcagca gggggagatt
    18901 cactatgaga gccgttcgct gtacgcaatg agcgaggccg accgccgtcg cctgctgcgc
    18961 accgaatggg gcgtagtgca tcagcatcca ctcgacggcc tgcgccgtca ggtctcggca
    19021 ggcggcaata tcggcgagcg gctgatggcg accggggcac gtcattacgg cgatattcgt
    19081 gccaccgcgc agaagtggct ggaagaggtg gaaattcccg ccaaccggat cgacgacctg
    19141 ccgaccacct tttccggcgg catgcagcaa cgtttgcaga tcgcccgcaa cctggtgacg
    19201 catccgaagc tggtgtttat ggacgagccg accggcggac tggatgtgtc ggtacaggca
    19261 cgcctgctcg acctgctgcg cggcctggtg gtggagctga acctcgcggt agtgattgtc
    19321 acccacgatt taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg
    19381 caagtggtgg agagtgggtt aaccgaccgc gtgctcgacg acccgcacca tccgtataca
    19441 caactgctgg tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc
    19501 ttatccggcc tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg
    19561 acacccgaac cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc
    19621 tgcaccagca aaacggcgtg cgcctgcccg tcttacaacg tgcctcgctc accgtcaacg
    19681 cgggcgaatg cgtggtgctc cacggccatt ccggcagcgg caaatcaacc ttgctgcgct
    19741 cgctgtacgc caactatctg cccgacgaag ggcaaatcca gatcaaacac ggtgacgaat
    19801 gggtcgatct ggtcaccgcg ccagcgcgca aagtggtgga gatccgcaaa accaccgtcg
    19861 gctgggtgag ccagtttctg cgcgtcatcc cgcgtatttc agcactggaa gtggtgatgc
    19921 agccgctgct cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc
    19981 tcacccgcct gaacgtgccg gaacgcctgt ggcacctggc gccatcaacc ttttccggtg
    20041 gcgaacagca gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc
    20101 tcgacgaacc taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc
    20161 gcgaagccaa aacccgtggc gcggccatcg tcggcatctt ccatgacgaa gccgtacgta
    20221 atgacgtcgc cgaccgcttg cacccaatgg gagcctctgc atgattatca ataacgtcaa
    20281 actggtgctg gaaaacgaag tggtacacgg ttcgctggag gtgcaggatg gcgaaatccg
    20341 cgcctttgcc gaaagccaga gccgcctgcc agaagcgatg gacggcgaag gtggctggct
    20401 actacccggc ctgattgagc tgcataccga caacctcgat aaattcttca ccccgcgccc
    20461 gaaagtcgac tggcccgccc attcggcgat gagcagccac gacgcgctga tggtggcaag
    20521 cggcatcacc accgtactgg acgccgtggc gattggcgac gtgcgcgacg gcggcgatcg
    20581 gctggagaat ctggagaaga tgatcaacgc catcgaagag acgcaaaaac gcggcgtcaa
    20641 ccgcgccgag caccgcctgc acctgcgctg cgaactgccg catcacacca cactgccgct
    20701 gtttgaaaaa ctggttcagc gcgaaccggt gacgctggtg tcgctgatgg accactcacc
    20761 gggccagcgc cagttcgcta accgcgagaa gtatcgcgaa tattatcagg gcaaatactc
    20821 cctcaccgat gcgcagatgc agcagtacga agaagagcaa ttggcgctcg ccgcacgctg
    20881 gtcgcagccg aatcgcgaat ccatcgccgc cctgtgccac gcgcgacaaa ttgcgctcgc
    20941 cagccacgat gacgccacgc acgcccacgt tgccgaatcc caccaacttg gcagcgtgat
    21001 cgccgaattt cccaccacgt tcgaagcggc ggaagcctcg cgtaagcatg gcatgaacgt
    21061 gctgatgggt gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg tggcggccag
    21121 tgaactggcg cagcttggtc tgctggatat cctctcttcc gactactacc ccgccagcct
    21181 gctggatgcg gcgttccgcg tcgccgatga cgagagcaac cgctttacgc taccgcaggc
    21241 ggtgaggctg gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc gcggggtgat
    21301 tggcgagggt aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc acattcatat
    21361 cgaccacgtc tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta
    21421 atggggccgt ccggctccgg gaaagacagc ctgctggcgg aactccgcct gcgggaacaa
    21481 acgcagttac tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagtgaaaac
    21541 catatcgccc tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct gctggcctta
    21601 agctggcacg ctaacggtct gtattatggc gtcggcatcg agattgacct ctggctgcac
    21661 gctggattcg acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc acgggcgcgc
    21721 tatcaatcgg cgctgctgcc cgtctgttta caggtttcgc cggagatcct gcgccagcgc
    21781 ctggaaaacc gtggccgtga aaacgccagt gaaattaacg cccggctggc gcgcgccgcc
    21841 cgctatactc cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc
    21901 gacacactgc tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg
    21961 cccggccacg cagtacgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc
    22021 ggagtttgac caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccgaacat
    22081 gcgctaccat ctggcgctgc tggatggcga agttgtcggc atgatcggcc tgcatttgca
    22141 gtttcatctg catcatgtca actggatcgg cgaaattcag gaactggtgg tgatgccgca
    22201 ggcgcgcggc ctgaacgtcg gtagcaagtt gctggcgtgg gcagaagaag aagcccgcca
    22261 ggccggggcc gaaatgaccg agctttcgac caacgtgaag cgccacgacg cgcaccgttt
    22321 ctatctgcga gaaggctacg agcagagcca cttccgcttc accaaggcgc tgtaacatga
    22381 gcctgaccct cacgctcacc ggcactggcg gcgcacaggg cgttccggca tggggctgcg
    22441 agtgcgcggc ctgcgccaga gcgcggcgct cgccgcagta tcgccgccag ccatgcagcg
    22501 gcgtagtgaa gtttaacgac gcgatcaccc tgatcgacgc cgggcggcac gatctcaccg
    22561 atcgctggtc gcctggatcg ttccagcagt ttttgctgac gcactaccat atggatcacg
    22621 tccaggggct atttccgcta cgctggggcg tgggcgatgt gatcccggtc tacggcccgc
    22681 cggatgaaca gggctgcgac gatctgttta aacatccggg cctgcttgat ttcagccaca
    22741 cagtggaacc gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta
    22801 accactcaaa actgaccttc ggttatctgc tggaaacggc acatagccgg gtggcgtggc
    22861 tgtctgacac cgctggcctg ccggaaaaaa cgctgaaatt tttactcaat aatcatccgc
    22921 aggtgatggt gattgattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg
    22981 atttaaatac cgtgcttgcg ctgaatcagg ttatccgctc gccacaggtg attctgaccc
    23041 atatcagcca ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg
    23101 tggggtttga tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc
    23161 cctcacccta accctctccc cagaggggcg aggggaccga ttgtgctcaa tattgaatat
    23221 tgcgctcgtt ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac
    23281 ctcatcgccc atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct
    23341 gatcaagctg gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc
    23401 ggtcgtcata ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg
    23461 aatcttcata gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat
    23521 taccaatagt atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg
    23581 tgaataaaag aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca
    23641 cattagtcat tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat
    23701 tgccgtaatc gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca
    23761 cgcatccaca atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagtgc
    23821 aacttcactg gcataatgcg gataatcagt gcgctctgac gacctggtgt ctttatcaat
    23881 cacctcactc attgaccatg aataaaaggc tggcacagca acacagaatc ctgcggtgcc
    23941 agcgacatga ccgcccattg ctccggtacg ttttttaaaa agaaacaccg gttgcattga
    24001 taaattgtgc tcagtatttg ccgaagtaca atatatataa taaatatata aaacacctca
    24061 tattatttac agagataata aattcgcgta attttccccc ttcagcacaa atgcctaata
    24121 aaatggatcg ttccgctcac acttccactt attaaagtcg caaattccca attatgataa
    24181 cgccagcttt aaatgacgta ttttgcatgg attacatatt tacgacacaa atgataaatg
    24241 gagatttata tgaataactc tcggttattc cgtttgagca ggattgttat tgcgttaact
    24301 gctgccagcg gcatgatggt aaataccgct aacgcgaaag aggaagcgaa agccgccact
    24361 caatataccc aacaggttaa tcagaattac gccaaatcat taccgtttag cgatcgtcag
    24421 gattttgacg acgctcagcg tggatttatc gctccgctgc tggatgaagg tattctgcgc
    24481 gatgctaatg gcaaaccata ttatcgcggg gaagattata aatttgatat caatgcgccc
    24541 gcgcccgaaa ccgttaaccc tagcctgtgg cgtcagtcgc agttaaacgg tatttccggc
    24601 ctgtttaaag tcaccgacag aatgtaccag gttcgcggtc aggatatctc caacatcacc
    24661 tttatcgaag gtgataccgg gattattgtc atcgacccgc tggtgacgcc accgagcgca
    24721 aaagccgccc ttgaccttta cttccaaaat cgcccgcaaa aaccgattgt tgcggttatt
    24781 tatacccaca gccataccga ccactatggc ggcgtgaaag gcattatctc tgaagctgat
    24841 gtgaaatccg gtaaagtgca ggttatcgcc cctgctggct ttatggacga agccatcagc
    24901 gaaaacgtac tggcgggtaa tattatgagc cgccgtgcac tttactccta cggcctgctg
    24961 ctggcgcata accctcaggg taacatcggc aatggtcttg gcgtaacgct ggcatcgggc
    25021 tacccgagca tcatcgcacc gaacaaaacc atcaccaaaa ccggtgagaa gatgattatc
    25081 gacggcctgg agtttgactt cctgatgacc ccaggtagcg aagcaccagc cgaaatgcac
    25141 ttctatattc cggccctgaa agcgctgtgt accgccgaga acgccacgca taccctgcac
    25201 aacttctaca ctctacgcgg cgcgaaaacc cgcgacacca gcaagtggac cgagtatctg
    25261 aacgaaacgc tggatatgtg gggtaacgac gcggaagtcc tgtttatgcc gcacacctgg
    25321 ccggtctggg gcaataagca tatcaatgat tatattggta aatatcgcga tactatcaag
    25381 tacattcacg accagaccct gcacctggcg aaccagggct acaccatgaa tgaaatcggc
    25441 gacatgatta aactgccgcc tgcacttgcc aataactggg ccagccgtgg ctattacggt
    25501 tctgtcagcc acaacgcccg cgcggtgtat aacttctatc ttggctatta cgacggtaac
    25561 ccggctaacc tgcatccgta tggtcaggtg gagatgggta aacgttacgt gcaggcgctg
    25621 ggcggttctg cccgtgtcat caacctggcg caagaagcga acaagcaagg tgattaccgc
    25681 tggtcggcag aactgctgaa acaggtgatt gccgccaacc cgggtgacca ggtcgcgaag
    25741 aatctgcaag cgaataactt tgaacagctg ggctatcagg ccgagtccgc cacctggcgc
    25801 ggtttctacc tgaccggcgc gaaagagctg cgcgaagggg tgcataagtt cagccacggc
    25861 accaccggtt ccccggacac cattcgcggg atgtcggtcg aaatgctgtt cgactttatg
    25921 tccgttcgcc tcgatagcgc gaaagccgcg ggtaaaaata tcagcctgaa cttcaatatg
    25981 agcaatggcg ataacctcaa cctgacgctg aacgatagcg tgcttaacta ccgtaaaaca
    26041 ctgcaatccc aagctgacgc ctctttctac atcagccgtg aagatctgca cgccgtgctg
    26101 accgggcaag ccaaaatggc ggatctggta aaagcgaaga aagccaaaat tattggcaat
    26161 ggcgcgaaac tggaagaaat tatcgcctgt ctggataatt tcgatttgtg ggtgaatatc
    26221 gtaaccccaa atta
//
LOCUS       DAZKGS010000060        26143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKGS010000060 DAZKGS010000000
VERSION     DAZKGS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            391..765
                     /gene="tomB"
                     /locus_tag="ACHOQY_002389"
     CDS             391..765
                     /gene="tomB"
                     /locus_tag="ACHOQY_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9678473.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            791..1009
                     /gene="hha"
                     /locus_tag="ACHOQY_002390"
     CDS             791..1009
                     /gene="hha"
                     /locus_tag="ACHOQY_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9678474.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1181..1732
                     /gene="maa"
                     /locus_tag="ACHOQY_002391"
     CDS             1181..1732
                     /gene="maa"
                     /locus_tag="ACHOQY_002391"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9678475.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1848..2318
                     /gene="ylaC"
                     /locus_tag="ACHOQY_002392"
     CDS             1848..2318
                     /gene="ylaC"
                     /locus_tag="ACHOQY_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9678476.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2482..4032
                     /gene="pdeB"
                     /locus_tag="ACHOQY_002393"
     CDS             2482..4032
                     /gene="pdeB"
                     /locus_tag="ACHOQY_002393"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9678477.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4074..4427)
                     /gene="ybaA"
                     /locus_tag="ACHOQY_002394"
     CDS             complement(4074..4427)
                     /gene="ybaA"
                     /locus_tag="ACHOQY_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9678478.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4547..4643)
                     /gene="ffs"
                     /locus_tag="ACHOQY_002395"
     ncRNA           complement(4547..4643)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOQY_002395"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4805..5116
                     /locus_tag="ACHOQY_002396"
     CDS             4805..5116
                     /locus_tag="ACHOQY_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9678479.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5147..5719)
                     /gene="ybaY"
                     /locus_tag="ACHOQY_002397"
     CDS             complement(5147..5719)
                     /gene="ybaY"
                     /locus_tag="ACHOQY_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9678480.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5937..6797
                     /gene="tesB"
                     /locus_tag="ACHOQY_002398"
     CDS             5937..6797
                     /gene="tesB"
                     /locus_tag="ACHOQY_002398"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9678481.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6846..8132)
                     /gene="amtB"
                     /locus_tag="ACHOQY_002399"
     CDS             complement(6846..8132)
                     /gene="amtB"
                     /locus_tag="ACHOQY_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9678482.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8162..8500)
                     /gene="glnK"
                     /locus_tag="ACHOQY_002400"
     CDS             complement(8162..8500)
                     /gene="glnK"
                     /locus_tag="ACHOQY_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9678483.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8681..10462)
                     /gene="mdlB"
                     /locus_tag="ACHOQY_002401"
     CDS             complement(8681..10462)
                     /gene="mdlB"
                     /locus_tag="ACHOQY_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9678484.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10455..12227)
                     /gene="mdlA"
                     /locus_tag="ACHOQY_002402"
     CDS             complement(10455..12227)
                     /gene="mdlA"
                     /locus_tag="ACHOQY_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9678485.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12257..12715)
                     /gene="decR"
                     /locus_tag="ACHOQY_002403"
     CDS             complement(12257..12715)
                     /gene="decR"
                     /locus_tag="ACHOQY_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9678486.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12868..13686)
                     /gene="cof"
                     /locus_tag="ACHOQY_002404"
     CDS             complement(12868..13686)
                     /gene="cof"
                     /locus_tag="ACHOQY_002404"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9678487.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13786..15486
                     /gene="ybaE"
                     /locus_tag="ACHOQY_002405"
     CDS             13786..15486
                     /gene="ybaE"
                     /locus_tag="ACHOQY_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9678488.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15551..16246
                     /gene="queC"
                     /locus_tag="ACHOQY_002406"
     CDS             15551..16246
                     /gene="queC"
                     /locus_tag="ACHOQY_002406"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9678489.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(16298..16696)
                     /gene="fadM"
                     /locus_tag="ACHOQY_002407"
     CDS             complement(16298..16696)
                     /gene="fadM"
                     /locus_tag="ACHOQY_002407"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9678490.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16790..17161)
                     /gene="ybaV"
                     /locus_tag="ACHOQY_002408"
     CDS             complement(16790..17161)
                     /gene="ybaV"
                     /locus_tag="ACHOQY_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9678491.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17312..19183)
                     /gene="ppiD"
                     /locus_tag="ACHOQY_002409"
     CDS             complement(17312..19183)
                     /gene="ppiD"
                     /locus_tag="ACHOQY_002409"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9678492.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19375..19665)
                     /gene="hupB"
                     /locus_tag="ACHOQY_002410"
     CDS             complement(19375..19665)
                     /gene="hupB"
                     /locus_tag="ACHOQY_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9678493.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19856..22210)
                     /gene="lon"
                     /locus_tag="ACHOQY_002411"
     CDS             complement(19856..22210)
                     /gene="lon"
                     /locus_tag="ACHOQY_002411"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9678494.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22398..23672)
                     /gene="clpX"
                     /locus_tag="ACHOQY_002412"
     CDS             complement(22398..23672)
                     /gene="clpX"
                     /locus_tag="ACHOQY_002412"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9678495.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23798..24421)
                     /gene="clpP"
                     /locus_tag="ACHOQY_002413"
     CDS             complement(23798..24421)
                     /gene="clpP"
                     /locus_tag="ACHOQY_002413"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9678496.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24668..25966)
                     /gene="tig"
                     /locus_tag="ACHOQY_002414"
     CDS             complement(24668..25966)
                     /gene="tig"
                     /locus_tag="ACHOQY_002414"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9678497.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
BASE COUNT         6313 a         7081 c         6245 g         6504 t
ORIGIN      
        1 atattaagta gcgctaatag atttaataat ccataatcat ttagaggcta ttcttaatta
       61 tttgcggtaa ttctttattc attcctcggt tattacgtca tattcagagc aatcctggta
      121 ttagtgtcac caatttcatc tggcgataat cctgaaatgt tatgaacagt tcgagcaaac
      181 tgcttttacc tgctgcgggt tagtgctaat atgaaaaagt gagtcctgtc ccgattcctt
      241 cctaattgta atttttcgta ataatgcgat gaaaacctgc aaagagtggc ttatagttaa
      301 gctaacaaac gagagggcaa gtccaggtca gtaagttttt tccatcccga aaggtgtccg
      361 ttagttcaac cactaagaag gggacgcgtt atggatgaat actcacccaa aagacatgat
      421 atcgcacagc ttaagtttct ctgtgaaacc ctgtatcatg actgtcttgc aaaccttgaa
      481 gaaagcaatc atggctgggt taacgaccca acctcggcga tcaacctcca gttgaatgaa
      541 ctgattgagc atattgcgac cttcgcactt aattacaaaa ttaagtataa tgaagacaat
      601 aagctcattg agcagatcga cgaatatctg gatgacacct ttatgttgtt cagcagttat
      661 ggtattaata tgcaggatct tcagaaatgg cggaagtcag gtaatcgact attccgttgt
      721 tttgtcaatg cgacgaaaga gaatcctgcg agtttatctt gttagaatta ttacaaccat
      781 gggtagaagt atgtccgaaa aacctttaac gaaaaccgat tatttaatgc gtttacgtcg
      841 ttgccagaca attgacacgc tggagcgtgt tatcgagaaa aataaatacg aattatcaga
      901 taatgaactg gcggtatttt actcagccgc agatcaccgc ctcgccgaat tgaccatgaa
      961 taaactatac gacaagatcc cttcctcagt atggaaattt attcgctaat aaataattcg
     1021 ctttcggagc tataaccggc tgtttattaa gaattttata ctttttcacc atgaagacat
     1081 accctatgtg atctttatca cacagatgta ataggaacgt tctcttcact gacttttcgt
     1141 cttactgtgt tgccgcattt tcagcaaccg gaggtcagta atgagcacag aaaaagaaaa
     1201 gatgattgct ggtgagttgt atcgctcggc agatgagacg ttatctcgcg atcgcctgcg
     1261 cgctcgtcag cttattcacc gatacaatca ttccctggcg gaagagcaca cattacgcca
     1321 gcaaattctc gctgatctat tcggtcaggt gacagaggct tatattgagc caacgtttcg
     1381 ctgtgactat ggctataaca tttttctcgg taataatttt ttcgccaact tcgattgcgt
     1441 gatgcttgat gtctgcccta ttcgcatcgg tgataactgt atgttggcac caggcgttca
     1501 tatctacacg gcaacacatc ccatcgaccc tgtagcacgt aatagcggtg ctgaactggg
     1561 gaaacccgtc accatcggta ataacgtctg gattggcgga cgcgcggtca ttaaccctgg
     1621 tgtgaccatt ggtgataacg tcgtggtagc ctcaggtgca gttgtcacaa aagatgtccc
     1681 ggacaacgtt gtcgtgggcg gtaatccagc cagaataatt aaaaaattgt aatcggtttt
     1741 tcgcaactgt tatgcaaaat tgtggtagat ctgttacttc ccctctacta ttcccacgtt
     1801 aaaatagggt gttccctgga aagttgcaga taccacgaag gcaaacgatg accgaaatac
     1861 aacgcctgct gaccgaaacg attgagtctc tgaatacccg cgaaaaacgc gacaacaaac
     1921 cccgctttag tatcagtttt atccgtaaac atccggggct gtttatcggt atgtacgttg
     1981 ctttttttgc caccctggcg gtgatgttgc agtccgaaac gctgtcaggc tctgtctggc
     2041 tactggttgt attatttatc ctgcttaatg gtttcttctt tttcgatgtc tacccacgct
     2101 accgctatga agatatcgac gtgctggatt tccgcgtttg ctataacggc gaatggtaca
     2161 acacgcgctt tgtacctgcc gcgctggttg aagccatctt gaactctccg cgtgtcgcgg
     2221 atgttcataa ggaacaactg caaaaaatga tcgtccgtaa aggtgaactg tctttttacg
     2281 atatttttac cctcgctcgc gccgaatcaa catcttaagt tagggttaca taccaggcgt
     2341 aaagctctgc gcctggtcaa atgacaatga tcgtttccac ccatcacttc atgaaatacc
     2401 agctctacct ccttatctcc tgccagcctt tttccacaat cagatatact ttccctacac
     2461 tgtgttaata aggatatgct ggtgagaaca cgacatctgg tcggccttat ttcgggagta
     2521 ctgattcttt cagtattgct gcctgtcggc ttaagcatct ggctggccca tcagcaggta
     2581 gaaacatcgt ttattgaaga gctggatacc tattcctccc gcgtcgctat tcgggccaat
     2641 aaggtggcga cacaagggaa agatgcgctg caagagctgg aaagatggca aggcgctgcc
     2701 tgtagcgaag cccatctcat ggaaatgcgt cgggtatctt acagttatcg ctatattcag
     2761 gaagtggttt atatcgataa caacgttccc cagtgttcgt ctctggagca tgaaagtccg
     2821 cccgatacct tccccgagcc aggtaaaatt tcgaaagatg gttatcgtgt ctggttaaca
     2881 tcgcataacg atttaggcat tatccgttac atggtcgcca tgggaacggc acattatgtc
     2941 gtcatgatcg accccgcttc ctttattgat gtcattccct atagctcatg gcaaattgat
     3001 gccgccatta ttggcaatgc ccataacgtt gtcataacca gcagcgatga aattgctcag
     3061 ggaattatta ccaggctaca aaaaacaccc ggtgagcata tcgaaaataa tggaatcatt
     3121 tacgatatcc tgcccttccc ggagatgaat atttcgatca tcacctgggc ttcaacgaaa
     3181 atgttgcaga aaggctggca tcggcaagtc tttatttggt taccgctcgg gttggtgatt
     3241 ggccagctgg cagcgatgtt tgtgctgcgt attttgcgcc gtatccagtc accgcatcat
     3301 cggctgcagg atgctatcga aaatcgtgat atttgcgtgc actatcagcc gattgtctcc
     3361 ttagccaatg gcaaaattgt cggtgctgag gcactggcgc gctggccgca gacagacggt
     3421 agttggttgt caccagatag ttttattccg ctggcacagc aaacgggcct ttctgagcca
     3481 ttgacgctac tgattataag aagcgtcttt gaagatatgg gcgactggct gcgtcagcat
     3541 ccacagcagc atatttcgat caatcttgaa tccaccgtgc tcacctcgga aaaaatcccg
     3601 caattgctgc gtgaaatgat caatcactat caggttaatc ccagacagat cgcgcttgaa
     3661 ctcactgaac gcgagtttgc cgatccgaaa accagcgccc cgataatttc tcgctaccgg
     3721 gaggcgggcc atgaaattta tcttgatgat tttggtacgg gatattcaag tttaagttat
     3781 ttacaggatc tggatgtcga cattctgaag atcgataaat ctttcgttga tgcgctggaa
     3841 tataaaaatg tcacgccgca tatcatcgaa atggcaaaaa cactgaaact gaaaatggta
     3901 gcggagggaa tcgaaaccag taaacaagaa gagtggctac gccagcatgg cgtgcactac
     3961 ggtcagggct ggctctacag caaggcatta ccgaaagaag atttcttacg ctgggccgag
     4021 caacatttgt gaatttttgg gctgacggca cgccgcacag cccgtcacgc tacctattcg
     4081 tcgatgattg actcgaatcc gccatagatc attcgcttgc cgtcaaacgg catggactcg
     4141 ccgaactctt tcatccgtgg gtccgacatc atcttttgat tagcagcgtc gcggacctct
     4201 tttgaagggt attcaatcca gctaaagaca acctcttcat tctcttccgc tttcaccgcc
     4261 atacgaaaat cggtcacttt gccatccggt acatcgctgg cccagcattc gacaatacga
     4321 agcgcgccaa actctttaaa caatggcgca gccttagcgg ccatttcccg ataggcatcc
     4381 tttttatcgg caggaacggc aaccacaaaa ccatcaacat acttcatcag acatacctcc
     4441 gaagcgtact gcgcagccag acaattcttg cgacgtcagt tgtggttaaa gtttagccat
     4501 gttggttttt tttgacgaaa cggtgggagg cagaaatggg tgggggccct gccagctaca
     4561 tcccggcaca cgcgtcatct gccttggctg cttccttccg gacctgacct ggtaaacaga
     4621 gtagcgttgc gggagaacca acaggccccc attgagagcg ttgagaacca acgcgcaggc
     4681 gcattatcac tgctgccccg gctaattgca acccatcgcg tgccagatgc tggtttcttg
     4741 cgcaatgcga cttcactcgg gcgtttttcc agattatgct gaaaaattac ctcaggaaga
     4801 aaagatggaa aaagaagatt catttcccca acgcgtctgg caaatcgtcg ccgctattcc
     4861 cgaaggctat gtcaccactt acggtgatgt ggcgaaactg gcgggatcgc cccgcgccgc
     4921 gcgccaggtg ggcggtgtgt taaagcgtct ccctgaaggc agcaccttac cctggcaccg
     4981 ggtggttaat cgccacggca caatttcgct aaccggaccg gatttacagc gtcagcgaca
     5041 ggcattactg gcagaaggtg tgatggtatc gggaagcggg caaatcgact tgcagcgtta
     5101 tcgctggaac tactaaccct ctccgggcgg agagggttaa cgtcgcttaa tattgcgtag
     5161 gagctggaac tgccgaagac ggattcacct gagttggtga agtcgaaggt acggtagtcg
     5221 ttgcaccacc gctggcctga accggcacgg cggtttgctg caccggcacc aatgtcaggt
     5281 cggctttagt tccgcccttg ttgatcaccg gctgaacggt atcggtaata aataccagtt
     5341 tgtcattcac ggtaatcgcc gcactcaaca gaatacgcgc gttcggctga acatctgccg
     5401 gattaaatgg cagaacaaag ctgaatggtg actgtttacc ttcggtacgc accgctttct
     5461 gcgccagcac ttttgacggt gcatcggcta acgacgcgtc agaaagtgtc acggtcagca
     5521 cagcatcagg cggcagtgcg actttctgcc ggatccagac ggtaccggag acattcggtt
     5581 gctggatagc aggttgttgt gttgctgaaa tagatgtatt tgcagccggg gctggcgtct
     5641 gaatatccgc gcttttatct gcgcaagccg ccaacgcaat cgcaaccgct aaaccactgg
     5701 ccatgtgcac gagtttcatt catttctcct tattatcaat gcaccagcgg gctaactttc
     5761 ctcgccggaa gagtggttaa caaaatagta acgtcaacaa gtgtggcaca catcacgcat
     5821 ttctgcctgt aattagcccg taattcagac cattgcaccc atcggaccag tagcaaattt
     5881 gcgttatact caactcactt tggcttgctg cggcagcttt gttactggag agttatatga
     5941 gtcaggcgct aaaaaattta ctgacattgt taaatctgga aaaaattgag gaaggactct
     6001 ttcgcggcca gagtgaagat ttaggtttac gccaggtgtt tggcggccag gtcgtgggtc
     6061 aggccttgta tgctgcaaaa gagaccgtcc ctgaagagcg gctggtacat tcgtttcaca
     6121 gctactttct tcgccctggc gatagtaaga agccgattat ttatgatgtc gaaacgctgc
     6181 gtgacggtaa cagcttcagc gcccgccggg ttgctgctat tcaaaacggc aaaccgattt
     6241 tttatatgac ggcctctttc caggcaccag aagcgggttt cgaacatcaa aaaacaatgc
     6301 cgtccgcgcc agcgcctgat ggcctgcctt cggaaacgca aatcgcccaa tcgctggcgc
     6361 acctgctgcc gccagtgctt aaagataaat tcatctgcga tcgtccgctg gaagtccgtc
     6421 cggtggagtt tcataaccca ctgaaaggtc acgtcgcaga accacatcgt caggtgtgga
     6481 tccgcgcaaa tggtagcgtg ccggatgacc tgcgcgttca tcagtatctg ctcggttacg
     6541 cttctgatct taacttcctg ccggtagctc tacagccgca cggcatcggt tttctcgaac
     6601 cggggattca gattgccacc attgaccatt ccatgtggtt ccatcgcccg tttaatttga
     6661 atgaatggct gctgtatagc gtggagagca cctcggcgtc cagcgcacgc ggctttgtgc
     6721 gcggtgagtt ttatacccag gacggcgtgc tggttgcctc gaccgttcag gaaggggtga
     6781 tgcgtaatca caattaaaaa aaatagccgg aggtaaaacc gtccggctat tttttgcagt
     6841 gcttgttacg cgttataggc attctcgccg tgactgttga catccagccc ttctcgctcc
     6901 tgctcttccg gtacacgcag accaaccgtc agatccgcca gtttgtagcc aataaatgcc
     6961 accacaccgg accagacgat cgtaatggcg atgctttcca gctgtaccag caactgatgg
     7021 cccatcgtca caccttcagc gaagcccacg ccacccagcg aactggcggc aaaaatccca
     7081 gtcatgatac agccgacaat gccacaaacg ccgtgcacac cgaagacatc gcagggatca
     7141 tccacccgca gcaagcgttt gagcatagta acgccccaca agcccgccag accagctacc
     7201 acgccgataa tcaacgcgcc gccaacccca atgtagccac acgctggcgt cacgccgacc
     7261 agaccggcaa tcgcgccaga acacgctccc agcagagaag gcttaccacg cagcgcccat
     7321 tcaccgaaga tccagccaag aattgccgcc gccgttgcga ccacagtatt cacaaatgcc
     7381 agtgccgcga tttcattcgc cgtgcccgct gacccggcgt taaagccaaa ccaaccaata
     7441 tagagaatgg cagtaccggt gaagaccatt ggcaggttgt gcggtttaaa cgcctctttg
     7501 ccgaagccca cgcgtttgcc tatcagatac gcgcccacca gaccggcgat tgcggcgtta
     7561 atatgcacca ccgtgccacc cgcgaaatcc agcgcaccgt gagaagccag caaaccaccg
     7621 ccccacacca tatgcgcaat cggaatgtaa gagagcgtca gccataccac cacgaaaatc
     7681 aacacagctg agaagcggat tcgttccgcc agcgccccaa cgatcaagcc gacggtaatg
     7741 caggcaaacg agccctgaaa cgccacgtgg atatactgat aaatgctgcc catcaccgcc
     7801 gtcagttcga tgttttttag catcaaccag ttaatgttgc cgaagaagtt gttgccctca
     7861 ccaaacgcca gcgagtaacc gtaaaccacc cagagaatac agaccagtgc aaacgtcacc
     7921 gtcacctgcg tcagcatcga cagcacgttt ttgccgcgaa tcaacccacc gtaaaacagg
     7981 gcaatccccg gaatagtcat aaacagcacc agcgcagtac aaatcatcat aaacgcattg
     8041 tcggctttat cggccaccgc aggtgcagcc attaccagtc ccggcagcat cgccagtgaa
     8101 gcaagcccag tttttatcgt cgctatcttc atttttcgtt cctgttgctg tgtgccagag
     8161 attacagcgc cgcttcgtcg gcttcgccgg tacgaatacg aatgacgcgt tgcaattcag
     8221 cgacgaagat tttgccgtcg ccaatttttc cggtgtaagc cgccttactg acgatatcga
     8281 tcacttcatc gagttggtca tcagcaatcg ccacatcaat ttttactttt ggcaggaagt
     8341 tgacgctgta ttccgccccc cggtacagct cggcatgccc tttctgacgc ccgaaacctt
     8401 tcacttcggt gacggtcagg ccctgaatac caatggaaga taacgcttca cgaacgtctt
     8461 ccagcttgaa tggtttgatt atcacggtca ccagcttcat agatcccctc cggtcagaat
     8521 tcggtaatgg ttctgctaca cgaagaaggt attgcaagcg gtgtgccaga aatgaaaaac
     8581 gagaaagaga gcaggaagtt aagaagattg aaaatgaaaa cgcactatga cagtgcacag
     8641 tgcgttacat ttttgcacca acgatgaaat attgcgctat tcaggcgctc aatgattctt
     8701 cttcacgcac gctggctgcc agctcttcgc ccgcaagttg cagttgatac atctgccagt
     8761 agcgtccctg ggccgccagc agttgctggt gagtgccctg ctccacggct tgcccacgat
     8821 gaagcaccag aatggtgtcg gcatcaacaa tggtcgataa gcggtgagca atcaccacca
     8881 gcgtggtatg ttcacgcaca gccgccagag catgttgaat cgcctgttca gtaccggagt
     8941 caatgctggc ggttgcctca tcaaggatca ggatttgcgg cgtctccacc aacacgcgtg
     9001 ccagtgccag cagttgcttt tgcccgacgg agagattatt cccctgctcg cccagcggcg
     9061 tataaatacc atcactcatg ctgcgcgcca gctccgccag ttgcacggtt tccagcgcct
     9121 gccagacgcg ttcttcggag atatcacgcc ccagcgtcac gttggcgagg aaggtatccg
     9181 ccagcaccac cggatcttgc tgcaccattg ccacgccctg gcgcagcgcg ctgtgactta
     9241 gcgaacttaa tggacgacca tcaaggcgaa tctcaccttc cgttagcggg taatagccca
     9301 tcaataaact ggcgagggtg cttttgccac tgccggtatg cccgaccagt gccacaaagt
     9361 tacgcgaagg cacagagaga ttaatgttct ttagcaccag attgtcatcg cgataagcaa
     9421 atgacacgtt atcgacttcg atggtgccac tctgtaacgg gcgatcatca ttgccatatt
     9481 gctggcgcgg tccgtccatc agttcaaaca cgcgctcacc agcaacaaca gcctgttgca
     9541 gcatcgcctg ttgcgtggtc agttcgatta atggttcgtt aagtcgccca agatagctga
     9601 taaacgcata cagcacgccc acttcaatgg tgccgctagc ggagaagcca aacagcatca
     9661 acaagccaca aagaatgagc gatgaaaaca gactcagcag cggacgcagc agaaaaccgt
     9721 cgaggcgcag ggtttgcatc ctggccatat agtgtgaacg actggcctcc cccatacgtt
     9781 cgccaaatcg cgcctgctga cggaactgct ggataacgct catgccattg atgatttcgt
     9841 taaagccgtc gttgatatcc gctagatagg cgcgcacacg acggacaatc ggcgtgctgt
     9901 aacgctggta tatcaccatt accaccagca ccaccgggaa aatcattatc gccaccagcg
     9961 ccattcgcca gtcgaggctg aacatcgcca ccagcatcgc gcccaccagt gcggcgctac
    10021 gcaggacagt tgccactacg gtaacgtaga gatcgcggat cacttcagtg tcattagtga
    10081 cgcgggaaat cacctgcccg acgggttggg tatcaaactc gcttaatggc tggcgcaatg
    10141 cggcatccat cacgtcggta cgcaactgtt gcactacgcc tactgccgcc cgattaaaca
    10201 gcagcgactg cgcgtaatgt agcccggcgg caaacagttg tagcccaaca tacgccgcag
    10261 ccagccctgc aaccattttc aacggcaggt tatttttcgc taccatattg tcgataaaat
    10321 agctgataag cagcggccca ctgacttctg ccgctgccgc aacccacatc atcaggaccg
    10381 caatccccag cggtttacgc cacggcgaac cgtacgctaa cagacgcttg agagtcggcc
    10441 acagttggct aaaactacgc atcgacggcc tcctcgcgaa tttccggagc gtcgtcgagc
    10501 gccgcctcca gttgttgata gcgatacata tcgcgatacc agccgctttg ttgcgccagc
    10561 tcatcatgat tgccacgctg ggcgatatgt ccgtgctgca tcacaataat ttcactggct
    10621 tccgtcagtg ccgaaaggcg atgggcactg atgattaccg ttctaccctg tccccactga
    10681 cgcaggttat gcaggatctg gtgctctgtg cgtccgtcca ccgccgaaag cgcatcatca
    10741 aggatgagga tttccgcgtt gactaataac gcacgagcaa tagagatacg ctgtttttgc
    10801 ccgccggaaa gcatcacgcc gcgctcgccc acctctgtat cgtaaccttg cggtaaacgc
    10861 aaaatatcgt catgtacgct ggctaaccgc gcgacatgct caatctcttg ctgggtggca
    10921 ttcgggcaac ccaacgcgat gttgttcgct acggtgtcgg aaaaaaggaa cggtgtctgg
    10981 ctaactaccg ccaggcggct acgccagcta tcgagttgta acttcgtcag aggaatatca
    11041 tgaaagcgaa tatccccctc gctgacgtcg aaatgacgct gaatgagcga caacagggta
    11101 cttttgccgg aaccagtcgg cccgcagata cccagcatct gaccgggttt cagggcgaaa
    11161 ttgacgtttt ccagcgcacg atggtcagtc tgcggatacg tgaactggtg aatatttaca
    11221 tcaagttcgc cacgcccttc cggcacgggt tcgctaccat cgttcaccac cggcgcttcc
    11281 gccagcatcg cacgaatacg gctgtacgca gcactaccac gttccacaat gttaaacatc
    11341 catgccagcg ccagcattgg ccaaatcatc agacctaaat acatcataaa actggtgagc
    11401 tggcccagcg ttaaactgcc ctgcaccacc atccaactac cgccgccaat cgccagcaag
    11461 ttcgccatac caatcgcgat atagattgtc gggtcgaaac gagcatcaat acgcgccacc
    11521 cgcatgtttt ttttgccggt atcttcggca tccgcggcaa ataacgccga ctggcgatct
    11581 tccagaccaa aggctttgat catgcggata ctggtgaggc tttcctgggt gcggtcatta
    11641 agactggaaa acgccgcctg tgccagctta aagcgttcat gcaaggcatc gccgttgcgc
    11701 ttaatcatga tcgccatcac tggcatcggt aacagggcaa ataaggtcaa ctgccagcta
    11761 atttgcgtag acatcataat caacacagcg cagcccatta ccagtgaatc caccagcgtc
    11821 agcacccctt ctccggcggc aaacacgacg cgatcgacgt cattggtcgc acgagccatg
    11881 agatcacccg tgcgatgacg caggtaaaac tccgggtgct gccggcttag ctgacggtaa
    11941 taatcttcac gcagttcaac agccagttgg taagacgcgc caaacagcaa tacgcgccag
    12001 acataacgca ggagataaac cacaacggca atcagcacca tggtggcgat ccacatcagg
    12061 atcagcccgg tagtaaagtg ttgttctgtc acgccatcga caacaatacc aaccactttt
    12121 ggcggaacca gttgcagcat cgcgataatg acaagcaagg cgacagcccc gagatagcga
    12181 cgccattccc gacggaaata ccagcttaat tgagcaaata atcgcacgcg ttgtgtcctg
    12241 acctgattct ggatatttat tcgatgggta aagaagtggt gtatttaatc tgttccatcg
    12301 cgaagctgga agtgacgtcc gacagccccg gcacgctgtt taccagacgc ttataaaact
    12361 cgtcgtagcg tttcatgtcg gcaacctgga cgcgcatcag ataatcgtat tcaccagcca
    12421 tgcgccagaa ccccagcact tctggcattt cggtaaccac cgtgacaaag cggcaatacc
    12481 attcgctgct gtgatgttgc gtttttatca gcacaaaagc ggtcaggccg aggcctattt
    12541 tttccggatc cagcagggcg actttgccga taaggatacc gtcgtcctcc agccgtttca
    12601 gacgcttcca gcaaggggtg gttgtcagat taacggcttc agccagtgcc tgcaaagaga
    12661 gggtgcaatc ctgctgcagt aaggccagca gcttatggtc aattttatct aacatagccc
    12721 ttccacagag aatttttttc tcttttaatt ctattttaaa ggtgaaatca cgacaattta
    12781 ttctgtgcgg tttaaacacc acggcacaaa atgacaaatt caacgcaaaa aaaaccgggc
    12841 aattgcccgg tgctggaagg gatctcgtta ttcgggggaa taaggtagat gtggatagtc
    12901 cagccagtgc gtcaaatagt gagagacagc ctgatttcgg caatgtccaa tcaccggtaa
    12961 atgcgggagc tccgcgcgca gttgcggcat cgcattgccc ataataaacc cgctaccgac
    13021 gctgcctaac atttcgcgat cgttcatcgc atcaccaaag gccatgcaat cgcgcaacga
    13081 taaacctaaa tgttgggtca gcaccgtcaa tgcagcgcct ttattgcagc ccaccggcag
    13141 cacttcgagg caatccgtgg cggaaaaaca caaatgtgca cgctcgccta atgcttcgta
    13201 tagctggatc tgcaagcgtg taagatcgtc gtgaccgcca cagaagcaga tcttggtgac
    13261 tctgccgagt ggcatttttt tgacatcgat tatctgataa cgaaaaccgc tatagacaaa
    13321 tgcctgcaac aacgcaggga tctctttccc ggtaaaccaa ccgtcgtcat tgaagatatg
    13381 catgctggct cgggtatccc attgctgata cagcaccagc tccgcgacat ccgcaggtaa
    13441 atcatcacga tgtaaaagtt caccttccag agaatgcacg cgcgttccgt tgccggtaat
    13501 caaatatgca tccagcgata gcgccccgag aatatgctgc atctccagcg catgacgccc
    13561 cgtggcaaaa gtgagggtaa tgtcgcgttc acgcagtcgc gccaaagtag agagggtttt
    13621 ctcacctaaa tgatggtcag gcatcaataa agtgccatcc atatcaaatg ctgccagacg
    13681 agccatttct ttctccactc tgtgacacgg ttaatggtga ttgagtatca cctgatatat
    13741 acggaagtaa tagtgaatag ttaaataata ttgttccggg tttatatgcg attgctcaac
    13801 cgtcttaacc agtatcaacg tctgtggcaa ccttcagccg gaaagccgca aaccgtcacc
    13861 gtcagcgaac tggccgaacg ctgtttttgc agcgaacgcc atgttcgtac gctgttgcgt
    13921 caggcacagg aggcgggatg gctggagtgg caggcgcagt caggacgcgg aaagcgcgga
    13981 caattacgct ttctggtcac gccggaatcg ctacgcaatg cgatgatgga acaggcactg
    14041 gaaaccggaa agcagcaaga tgtgctggag ctggcgcaac tggccccagg tgagctgcgc
    14101 actctgttac agccgtttat gggcggacaa tggcaaaacg atacacccac gttgcgtatt
    14161 ccctactatc gcccgctcga accgctacaa ccaggctttt tgcccggccg tgccgagcag
    14221 catctcgccg ggcagatatt ttccggcctg acccgcttcg ataataatac tcagcgcccg
    14281 attggcgatt tagcgcatca ctgggaaacc tctactgacg ggttacgctg ggacttttat
    14341 cttcgttcaa ccctacactg gcataacggc gatgcagtaa aagcctcaca cttacaccag
    14401 cgattattga tgctgttaca actgccagca ctggatcaat tatttattag cgtgaagcat
    14461 attgaagtca cccatccgca gtgtctgacc ttctttttac atcgccccga ttactggctt
    14521 gcgcaccggc tggcgagcta ttgcagccat ctggcgcatc cgcaattccc actgatcggc
    14581 actggtcctt ttcgcttaac acaattcaca gcggagctgg tgcgcctgga gagccatgat
    14641 tattaccatt tgcgtcatcc gctgcttaaa gcggttgagt actggataac tccgccgctt
    14701 ttcgaaaaag atttgggaaa cagttgtcgg catcccgtgc aaatcaccat cggcaaaccg
    14761 gaggagctgc aacgggtcag ccaggtcagt agtggcatca gtttaggttt ttgctattta
    14821 acgttgcgca aaagtccccg actctccctc tggcaggcgc gaaaagtgat ctccattatt
    14881 catcaatccg gtttattaca aacgttagaa gtcggagaaa acctgatcac cgccagtcat
    14941 gcattactgc caggctggac tattccgtac tggcaagtac cggatgaagt caaactaccg
    15001 aaaaccttga cgctggttta tcacctaccg atagaacttc ataccatggc agaacgccta
    15061 caggcgacac tggcagcgga aggctgtgaa ctcacaatta tttttcataa cgcaaaaaac
    15121 tgggacgaca cgaccctact ggcacacgca gacctcatga tgggcgacag attaattggc
    15181 gaagcaccgg aatatactct ggagcaatgg ctacgttgcg atccactatg gccacatgtt
    15241 ttcgacgctc cagcatatgc acatctgcaa tcgacgctgg acgcggtgca agtaatgcct
    15301 gatgaggaaa accgatttaa tgccctgaaa gcggttttta gccagttaat ggcagacgcg
    15361 acgctgacgc cgctgttcaa ctatcactat cgcattagtg cccctcccgg cgtgaacggt
    15421 gtgcgactga caccgcgcgg ctggtttgaa tttaccgaag cctggcttcc cgcgccgtcg
    15481 caatgaatag ctggtccggg ttaatcgcag gcgttaccat aacgctttta ttgttcaagc
    15541 aggattatct atgaaacgtg ctgtcgttgt gttcagtgga ggccaggatt ccaccacctg
    15601 tctggtgcag gcattacaac aatatgatga agtccattgc gtgacgttcg attacggtca
    15661 gcgacatcgc gcagaaatcg acgtggcacg cgaactggcg ctgaaactgg gagcacgcgc
    15721 gcataaggtt cttgatgtaa cgctgctcaa cgagctggcg gtcagcagcc tgacacgtga
    15781 cagcattccg gtgcctgatt atgaacctga agccgatggc atcccgaata cgtttgtccc
    15841 tgggcgtaat attttgttcc tgacgctggc ggcaatatat gcgtatcagg taaaagcaga
    15901 agccgtaatt accggcgtct gtgaaacgga tttctccggc tacccggatt gccgcgatga
    15961 gtttgtgaaa gcactaaacc atgccgtcag tttgggcatg gcgaaagata ttcgttttga
    16021 aacgccgctg atgtggattg ataaagcgga aacctgggcg ctggcagatt attacggcaa
    16081 actggattta gtccgtaacg aaacgttgac ctgctataac ggctttaaag gcgacggttg
    16141 cggtcattgt gcggcatgta atttacgcgc caacggtttg aatcattatc tggccgataa
    16201 accgacggtg atggcagcga tgaagcagaa aaccgggttg aggtaattat tccgggtgtc
    16261 gccggatgcg gcttgagcat ccggcaccac aaaacgttta cttaaccatc tgctccagct
    16321 tttcgcgcaa ttccccttcc agagctaatg ctttctgcgt tttaagatca atacaaacaa
    16381 acgtaataag cgcatccgct accacctgcc cttccggctc cagtgtaatg acctggctta
    16441 agatgccgct tttaccgttt aattgctgca actgactggt aatagttaac aggtcgctta
    16501 ataccgctgg gcgacgatag ttaatattga tattgaccac gacgaaggcg atgttatggg
    16561 ccgtcatcca ctgaaaactg tcgctatttt ccaacccatc ccagcgggct tcttcgagaa
    16621 attcaaggta gcgggcgttg ttgacgtgct ggtaaacgtc gagatgatat ccacgaactt
    16681 tgatttgtgt ttgcatagcg caataacctt acgttgttgt tataagtaca gaggtcataa
    16741 ctggtatgac ctcttcagtc tggcaaaaaa ttgccactat gcaaattaat tacagggtta
    16801 ataccgccag attacgttcc accagcgaat tgcccatccc cggcacctgc tttagatcct
    16861 ccaccgtttt aaacggaccg tactcttcgc gataactgac aatcgcctgc gctttcttca
    16921 ggccaacgcc attcatcgcg cgggctagct cttccgcgct ggcattatta atgctgaccc
    16981 gggtgccttc ttcgtcactg gctttcgccg gtactgctgc tttactttgt gctgcaggag
    17041 cttccgcttt ggtttctacc gccgtcggtt tcgccacaga agctgccgcc agcgcgctat
    17101 gagacattcc ggcacaggcc agggacaggg taatgagcag tgctttaatt ccgtgtttca
    17161 tactgttttc tccttgtttg ttaacagtgg aatcacctta acgggagtga aaacagtaaa
    17221 caaatggcaa tgttcagaaa tggaaaaggc cgcgaaagcg accttttctt gttacagctg
    17281 caacgcattt ttttgcgagg cggcttcagg attattgctg ttccagcgca tcgccaattt
    17341 tgattttcgc ctctttacgc aggttactca tcagagcttc aaagacgatt tgtgcgttgt
    17401 tctgggtgat accctgcacc atcgcttttt tctgatcttc cggcattgaa ccttgtttca
    17461 cttcatccag cgccagcaga accacattac cttgcatatc ggtcgccata ccgtagctcg
    17521 gtttgtcttt cgctggcagt ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg
    17581 aacggcttaa gattttcggc tcgccaaatt tcagaccggc agcctgcata gcttccgcac
    17641 ctttgccggc tttcaaatca accagcagtt tctcagcatc cactttcgcc tgttgttcag
    17701 ctttgttgtg ctgaaccagt gccttaactt gttcctgaac atctgccaac ggtttcaccg
    17761 cttccggttt gtgctcgctg atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga
    17821 tgtcagagtt gatgcccggc gcgccgtttt cacctaccag accgccgtta aagatagcgt
    17881 cggcaaccgg cttgaagttc aactcttccg gcaggttatc tttgctaaac caacccgtct
    17941 gagtggcttt aacgccggca gcttgctctg caccggccag agactccgtg tcgttgcttg
    18001 ccgcatcgct cactttctgc tgcagcgcat agtacgcatc gagggctttt tcgtgtttca
    18061 ctttcgccgc aatgtcgtca cgtacttcgt ctaacgattt cactttcgct ggctgaatgt
    18121 cgtccagacg tacaatcagg aaaccgaccg aagatttgat gacaccagac agttgccctt
    18181 tttctttcag accagcattt ttcagttcgt cggggatagt ggcatcttct aaccaaccca
    18241 tatcgccgcc gttacgggca gagataatat cggcagattt ttctttggct aatgcagcaa
    18301 aatcaccgcc tttattcagc tcatcaagta ccactttcgc ttcatcttca gttttggtct
    18361 ggatgatgct gtagcgggta cgctgcggct gggtgaattg atcctgatgc tgatcgtagt
    18421 agctctggat atccgcatcg ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt
    18481 aactcacgcg gaattgttcc ggcgtcatga aattgttttt gttttgttcg tagtagctgg
    18541 caatctcctg ttcggtcaca ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct
    18601 cacgcaccac gcgttgttgc gcgaccagtg ccgccagctc gtcggtttca cctttcagca
    18661 taaaatcggt accggcaaca ccgttaatca gctgttgggt agtgagctgg ttacgcagcg
    18721 cctgggcgta ctgatcggcg gtcatcccca tctggttgag gataccgtta tagcggctgt
    18781 tatcaaattt gccgtcaacc tggaaggctg gggtcgcgaa aatcgcctgt ttcacctgct
    18841 catcactgat acccagtttc agctcacgag cgtactgatc caacagcgcc tcgtcgatca
    18901 gacgattcag cacctgttga cgcagggttt tcatatagcc ttcgttcgct gccagctcgg
    18961 agtattgatc gcccagctgt tgctgcatgc gattacgctc gctgttaaag gcgttctcga
    19021 actgcccacg gctgatttcc tggtcattca cttttgcggc gtagttattg cctccgccaa
    19081 tcaggtaacc actcacgccg gtcaatatga acgacacgat aatgataccg aaaataatct
    19141 tgagcacgag actgtttgca gccgtgcgta agctgtccat catggtgtaa caacactccg
    19201 ctgtagatga ctgtgtacct cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa
    19261 gaaacggggg caattgtcag cccacaagac acagaaagtc accgaataca aataaaaaag
    19321 gcacatcagt agatgcgccc ttgaacttcg tcacatcccc actggggaca acgcttagtt
    19381 taccgcgtct ttcagtgctt tacctgcacg gaagctcggt actttagcag cagcgatggt
    19441 gatctcttta ccggtctgcg ggttgcggcc agtacgggca gcacgctctt taacggcaaa
    19501 agtaccaaaa cctaccagtg ctacatcatc cccttctttc agagattcag ttacggaagc
    19561 aataatagca tctaacgcac ggccagccgc agctttagag atatcagccc ctgcagcaat
    19621 cttgtcgatc aattgagatt tattcactct tctcttcctc tttataattt atatcgcact
    19681 tgaatccttc aaggtacgaa cgcgcagcag ttatatcagg ccagccatcc ccttacaaca
    19741 ccccttaata agggcaagcc cgatccgcca tctaacttag cgagacaaaa aaaggctggc
    19801 aagcccgaat tagcctgcca gccctgtttt tattagtgca ttttgcgcga ggtcactatt
    19861 ttgcagtcac aacctgcatg ccagacggtt cattttgcag cgccagagtc agaacttcct
    19921 caatgcgctt cacaggatga atgtccagat cggcaattac gttgtcagga atctcttcca
    19981 gatcgcgttt attttcgaac ggaattagca ctgttttaat cccgccgcga tgcgctgcca
    20041 ggagtttttc tttcaaacca ccgatcggca gtacctgacc acgcagagtg atctcaccgg
    20101 tcattgccac atcggcacga accgggttac cggtcaggca agaaaccagc gcggtgcaca
    20161 tagcaatacc ggcactcgga ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga
    20221 tgtcacgttt ttcgtaaaaa tcagggttga tccccagttt ttccgcacgc gcacgaacca
    20281 ccgttaacgc cgcctgaata gactcctgca tcacttcgcc gagcgaaccg gtataggtca
    20341 gtttgccttt acccggaaca catgcggttt caatggtcag caagtcaccg cccacttccg
    20401 tccacgccag accggttacc tgaccgacac ggttttcgtt atcagcgcga ccatagtcga
    20461 aacgctgaac accgaggtag tcatgcaggt tatcgccgtt aatttcgata tgttttaatg
    20521 acttatcgag cagtaactgc ttaaccgctt tgcggcacag tttggagatt tcacgctcca
    20581 gaccacgcac gcccgcctca cgggtgtagt aacgaataat gccgataatg gcgctatcgt
    20641 cgacggtcag ctcacctttt ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac
    20701 gtttggcgat gttcagtttt tcatcttcgg tataaccgga gaggcgaatc acttccatac
    20761 gatccagcag cggtgccgga atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc
    20821 tgagatcgta atccacttcc aggtagtggt cgctgaacgc tacgttctgc tctggatcca
    20881 gcacttcaag cagtgcagag gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct
    20941 catcgagcag gaacagcggg tttttcacgc ccactttcgc cattttctgg atcagtttac
    21001 ccggcataga accgatgtaa gtacggcggt gaccacggat ttccgcttca tcacgcacgc
    21061 cgcccagcgc catacggaca tatttacgcc cggtggcttt ggcaatggac tgaccaagag
    21121 aggttttacc tacccccggc ggccctacca ggcagaggat cggtcccttg attttgttga
    21181 cacggctttg aaccgcaagg tactcaagga tgcgatcttt cacgcgctcc agaccataat
    21241 ggtcggtatc aaggatttcc tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat
    21301 tccacggtac ctgtaccatc cagtcgatat aaccacgcac tacggtcgct tctgccgaca
    21361 tcggagacat cattttcagc ttctgcaact ctgcttccgc tttctctttt gcctctttcg
    21421 gcattttcgc cgcgtcgatt ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca
    21481 tttcaccgag ttctttctga atagctttca tttgctcgtt cagatagtac tcacgctggg
    21541 atttctccat ctgcttttta acgcggttgc gaatgcgttt ctcaacctgc agcagatcga
    21601 tttccgattc catcattgcc atcagatatt ccagacgttc gttaacgtcg gacatctcca
    21661 gaacggactg tttgtcagcc agtttcagcg gcatatgtgc agcaatggta tccgccagac
    21721 gcgccggatc gtcgatgcta ttcagcgacg tcagcacttc tggtgggatt tttttgttca
    21781 gcttgatgta gccttcgaac tggctgattg cagtacgcac cagcacttcc tgttcccgct
    21841 catcaatggt cggcgactcc agatactccg ccttcgcaga aaagtgttcg ccattgtcag
    21901 agagcgcaga aatacgcgcg cgctgtaacc cctcgaccag cactttgacg gtgccgtcag
    21961 gcagtttcag catctgcaat atagaggcca cggtcccgac ggtgaaaaga tcgtttacac
    22021 ccggctcatc cgttgaagct tctttctgcg cgaccagcat aattttttta tcatggtcca
    22081 tcgccgcttc cagacaacgg atagattttt cccgcccgac aaataagggg atgaccatgt
    22141 gcggataaac caccacatcg cgcagcggca atacggggat ttcaatgcgt tcagaacgct
    22201 caggattcat agagctctct cttagtttaa tttccgccag gtaatcagat gacacgactg
    22261 tgcttcacgc catctattaa catgtacgtc agtatatggg gatgtttccc ccacattcaa
    22321 cgccgagaat agaggaaaaa ttaaagggga gataaaatcc cccctttttg gttaactaat
    22381 tgtatgggaa tggttaatta ttcaccagat gcctgttgcg cttccggctt accataaatc
    22441 agcaacggtt tgctttgacc atcaattacc gactcgtcga taaccacttt ttcgacatct
    22501 tccatggacg gcagatcgta catggtatcg agcagtgcgg cttctacgat ggaacgcagg
    22561 ccacgggcac cggttttacg cgccatcgct ttcttagcga tagcatccag cgcctcgtca
    22621 cggaattcca gatccacgcc ttccagatta aacagcgcct gatactgctt ggtcagggcg
    22681 tttttcggct ctttgaggat ctgaatcaga gcttcttcgc tcagttcatt caacgttgcg
    22741 acaaccggca gacgaccaat aaactcaggg ataagaccaa acttgatcag atcttccggt
    22801 tcaacctgcg ccagcagctc gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg
    22861 ccaaaaccaa tgccggagcc ggtttctaca cggtgggaaa tcactttatc cagaccggca
    22921 aacgcaccgc cacaaataaa caggatctta gaggtatcaa cctgcaagaa ttcctgctgc
    22981 ggatgtttac gcccaccttg cggtggaaca gcagctaccg taccttcgat cagtttcaac
    23041 agtgcctgct gtacgccttc accggaaacg tctcgggtaa tggacgggtt gtctgactta
    23101 cgagaaatct tgtcgatctc atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg
    23161 taatcgcact tctgcaacag cttctgaatg atgttttcaa cgtcttcgcc cacataaccg
    23221 gcttcggtca gcgtggttgc gtcggccatg gtgaacggga cgtccagcag gcgcgccagc
    23281 gtttcagcca gcagcgtttt accggaaccg gtcggaccga tcagcagaat gttacttttg
    23341 cccaactcga caccattgct ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc
    23401 gcgaccgcca gcactttttt cgcctgttcc tggccgataa cgtaatcgtc caggtggttg
    23461 cgaatttcat gcggcgtcgg tagcgcactg cgttcacgat gcggtgcaac ttctttaatc
    23521 tcttcgcgaa tgatgtcgtt acataaatca acacattcgt cgcagatata cacggatgga
    23581 ccggcaatca gcttgcgcac ttcatgctgg cttttgccgc aaaaagagca atacagcaat
    23641 ttgcctgagc catctttgcg tttatctgtc atgggtcaaa acctcttctt tgttctttgt
    23701 gccgcacacg acgacgcaaa tgccattctc aggcgcaagc cgcttacagc gttgtgccgc
    23761 cctggataag tatagcggca cagttgcgcc tctggcatca attacgatgg gtcagaatcg
    23821 aatcgaccag accgtattcc accgcttcag gggcggaaag gaagcgatcg cgctcggtat
    23881 cacgttcaat ctgttctaat gattgacccg tatgaagcgc cataagttca ttcatgcgcc
    23941 ctttaacttt cagaatttca cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc
    24001 cgcccaacgg ttggtgaatc atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg
    24061 cccctgcggt caacaagaaa gcgcccatcg aggccgcctg gcccatacag atggtgctga
    24121 catcaggctt gataaactgc atggtgtcat agatagacat cccggcagtg atcaccccgc
    24181 ctggggagtt aatgtacaga tagatatctt tttctgggtt ttccgcttcc aggaacagca
    24241 tctgcgccac aatcaggtta gccatgtggt cttcaacctg gccagtcaga aaaatgacgc
    24301 gttccttaag tagacgagaa tagatatcaa aagagcgctc accgcgtgag gtctgttcaa
    24361 tgaccatcgg caccagcgcc atatggggtg caaagttatc tcgttcgccg ctgtatgaca
    24421 tttccgtctc ctggataaaa ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg
    24481 attatcagct agtccctgtg ttatgggtac ggcatcacca tatttcaagc ataacaatct
    24541 tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ttccatgcaa aattcatgac
    24601 aaaaaaaaag cccaccacct ggcggtgacg ggcctttgtg cgaatttagc gcgttatgct
    24661 gcgtaaatta cgcctgctgg ttcatcagct cgttgaaagt ggtttctttt tcagtcactt
    24721 tcgctttcgc cagtacagct tcaacagcct gttcttccag agcaacattg cgcatgttgt
    24781 ccatcagttc tttgtttttg ctgtagaact cgataacttc tttcggatct tcgtacgcag
    24841 aagccatctc ttcgatcagg cctttcacgc gctcttcgtc agctttcagc tcgttggtgc
    24901 ggataacttc gcccagcagc aggccaacaa ctacgcggcg tttagcctgt tcttcgaaca
    24961 gttcgcgcgg cagttccaga gcttgttttt cgttgccacc gaaacgctgt gcagcctggc
    25021 gacgcagaac gtcgatttcg ctgtcgatca gcgcagccgg tacgtcgatg tcgttagctt
    25081 ttaccagacc ttcgatcgcc tgagacttaa cgcggttacg gatggcgctc ttcagctcgc
    25141 gctccatgtt tttacgcact tcagcgcgca gaccttctac ggaaccatct tcaacgccga
    25201 aacgtttgat gaattctgca gtcagttccg gcagttcacg ctcttcaact ttcttcaggt
    25261 tgatagcgaa tttcgctgct ttacctttca ggttttctgc gtggtattct tccgggaagg
    25321 tcacgtcgat ggtgaactct tcgccagctt tgtggccttt gataccgtct tcaaagcccg
    25381 ggatcatacg accctggccc atcgccagta cgaaatcaga cgctttaccg ccttcgaact
    25441 cttcgccgtc tacagaaccg gtgaagtcga tggttacgcg gtcttctgct tcaacagcgc
    25501 cgtctttttc tttccaggtc gcctgctgtt tacgcagagt atccagcatg ccgtcaacgt
    25561 cagcgtcggt cacttcaacg atcggttttt caacttcgat cgcttccaga ccctgcagtt
    25621 caacttccgg ataaacttca aactctacag agtaagtgaa gtcttcaccc agcttgtatt
    25681 cgcccggaac ataagtcggt gcgccagccg gattgatttt ttctttaatg atggcgtcaa
    25741 tgaagttacg gctcatcagg tcacccagaa cgtcctggcg tacagacgcg ccataacgct
    25801 gagcaacgat attcattggc actttgcctt tgcggaagcc gtcaatacgt acttttttcg
    25861 caacgttgac cagctcgctt ttaacagcgg tctcgatgct gtcagcagcg atagtaatcg
    25921 ttacacggcg gccaaggcct tgagtggttt caactgaaac ttgcatcttg ttacctcaaa
    25981 aaatcacagt gctcggtcaa ctcttacccc gcaagcacag gttgttggct tcgcggaacc
    26041 gggatgttct tataatcaat cacattccct gttaccagaa tcatcccgaa agcattcaaa
    26101 taggacgcgg cattatagcg gcatcacttt tatgagtcga gaa
//
LOCUS       DAZKGS010000061        25240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKGS010000061 DAZKGS010000000
VERSION     DAZKGS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHOQY_002415"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHOQY_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9678498.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHOQY_002416"
     CDS             complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHOQY_002416"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9678499.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHOQY_002417"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHOQY_002417"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9678500.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="ACHOQY_002418"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="ACHOQY_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9678501.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="ACHOQY_002419"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="ACHOQY_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9678502.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="ACHOQY_002420"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="ACHOQY_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9678503.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="ACHOQY_002421"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="ACHOQY_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9678504.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHOQY_002422"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHOQY_002422"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9678505.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHOQY_002423"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHOQY_002423"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9678506.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9874..10239
                     /gene="yfiL"
                     /locus_tag="ACHOQY_002424"
     CDS             9874..10239
                     /gene="yfiL"
                     /locus_tag="ACHOQY_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9678507.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10389..10907
                     /gene="yfiR"
                     /locus_tag="ACHOQY_002425"
     CDS             10389..10907
                     /gene="yfiR"
                     /locus_tag="ACHOQY_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9678508.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10897..12123
                     /gene="dgcN"
                     /locus_tag="ACHOQY_002426"
     CDS             10897..12123
                     /gene="dgcN"
                     /locus_tag="ACHOQY_002426"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9678509.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12139..12621
                     /gene="yfiB"
                     /locus_tag="ACHOQY_002427"
     CDS             12139..12621
                     /gene="yfiB"
                     /locus_tag="ACHOQY_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9678510.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12698..13045)
                     /gene="rplS"
                     /locus_tag="ACHOQY_002428"
     CDS             complement(12698..13045)
                     /gene="rplS"
                     /locus_tag="ACHOQY_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9678511.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13087..13854)
                     /gene="trmD"
                     /locus_tag="ACHOQY_002429"
     CDS             complement(13087..13854)
                     /gene="trmD"
                     /locus_tag="ACHOQY_002429"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9678512.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13885..14433)
                     /gene="rimM"
                     /locus_tag="ACHOQY_002430"
     CDS             complement(13885..14433)
                     /gene="rimM"
                     /locus_tag="ACHOQY_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9678513.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14452..14700)
                     /gene="rpsP"
                     /locus_tag="ACHOQY_002431"
     CDS             complement(14452..14700)
                     /gene="rpsP"
                     /locus_tag="ACHOQY_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9678514.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14949..16310)
                     /gene="ffh"
                     /locus_tag="ACHOQY_002432"
     CDS             complement(14949..16310)
                     /gene="ffh"
                     /locus_tag="ACHOQY_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9678515.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16477..17268
                     /gene="ypjD"
                     /locus_tag="ACHOQY_002433"
     CDS             16477..17268
                     /gene="ypjD"
                     /locus_tag="ACHOQY_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9678516.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17289..18575
                     /gene="yfjD"
                     /locus_tag="ACHOQY_002434"
     CDS             17289..18575
                     /gene="yfjD"
                     /locus_tag="ACHOQY_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9678517.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18630..19223)
                     /gene="grpE"
                     /locus_tag="ACHOQY_002435"
     CDS             complement(18630..19223)
                     /gene="grpE"
                     /locus_tag="ACHOQY_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9678518.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19346..20224
                     /gene="nadK"
                     /locus_tag="ACHOQY_002436"
     CDS             19346..20224
                     /gene="nadK"
                     /locus_tag="ACHOQY_002436"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9678519.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20310..21971
                     /gene="recN"
                     /locus_tag="ACHOQY_002437"
     CDS             20310..21971
                     /gene="recN"
                     /locus_tag="ACHOQY_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9678520.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22120..22461
                     /gene="bamE"
                     /locus_tag="ACHOQY_002438"
     CDS             22120..22461
                     /gene="bamE"
                     /locus_tag="ACHOQY_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9678521.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22523..22813)
                     /gene="yfjF"
                     /locus_tag="ACHOQY_002439"
     CDS             complement(22523..22813)
                     /gene="yfjF"
                     /locus_tag="ACHOQY_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9678522.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22803..23240)
                     /gene="ratA"
                     /locus_tag="ACHOQY_002440"
     CDS             complement(22803..23240)
                     /gene="ratA"
                     /locus_tag="ACHOQY_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9678523.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23411..23893
                     /gene="smpB"
                     /locus_tag="ACHOQY_002441"
     CDS             23411..23893
                     /gene="smpB"
                     /locus_tag="ACHOQY_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9678524.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24108..24470
                     /gene="ssrA"
                     /locus_tag="ACHOQY_002442"
     tmRNA           24108..24470
                     /gene="ssrA"
                     /locus_tag="ACHOQY_002442"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24741..24989
                     /locus_tag="ACHOQY_002443"
                     /pseudo
     CDS             24741..24989
                     /locus_tag="ACHOQY_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
BASE COUNT         6452 a         6657 c         5911 g         6220 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaagcatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cattgatggc aaccagctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacga ctgatttatc cactattaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7081 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtgc tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcagaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttc
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgatttcaa cccttacttc tgtatagata aatttacact ccctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10201 ggggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
    10261 aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa
    10321 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
    10381 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
    10441 caccaatact cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
    10501 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
    10561 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
    10621 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
    10681 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
    10741 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
    10801 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
    10861 tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt
    10921 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
    10981 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
    11041 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
    11101 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
    11161 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcgtcctg gcattacacc
    11221 cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt ccccgccccc
    11281 atcattcagc cgattcgtca caatggtgaa accattggcg aagtacgctt aaccgctcgc
    11341 gacagttcaa tcagccattt tatctggttt tcgctcgccg tactgaccgg ttgtattctg
    11401 ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggcct ggtagaagcg
    11461 ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
    11521 gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctg
    11581 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
    11641 gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaat
    11701 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
    11761 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa
    11821 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
    11881 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
    11941 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
    12001 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgaa aaaattacaa
    12061 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
    12121 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta
    12181 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
    12241 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
    12301 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
    12361 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
    12421 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
    12481 gcatgggcta tgggtggaca aattccgcgc agcaatctca ccacacaggg tttaggaaaa
    12541 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
    12601 gtggtgatta ctacccctta aaacaccagc aaacagataa aaaaagggcc agccaattgg
    12661 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
    12721 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
    12781 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
    12841 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
    12901 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
    12961 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
    13021 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
    13081 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
    13141 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
    13201 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
    13261 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
    13321 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
    13381 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
    13441 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
    13501 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
    13561 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
    13621 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
    13681 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
    13741 aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
    13801 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
    13861 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
    13921 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
    13981 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
    14041 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
    14101 caggtctttc cagtagtagt ccccctcttc aagctgaggc agctgcgatg aatccacgac
    14161 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
    14221 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
    14281 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
    14341 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
    14401 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
    14461 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
    14521 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
    14581 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
    14641 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
    14701 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
    14761 attaaaagcc cgaaaatttt actcattttt gcgggaattg caatcaacag ttgctaactc
    14821 tgctgtaaaa ggccgtcggc ggtgcagcca gtttggtgcc ggagtgcgcg cagtcaccgg
    14881 agcgtacacg cagtacgtga ggatgacgag cacatcccgg tgccaaaatg gcaaacaagc
    14941 caggccgatt agcgaccagg gaagcctggg ggcatcatac ccttcatgct tctcatcatc
    15001 ttcgccattc cgcctttctt cattttcttc atcatgcgct gcatgtcgtc gaactgcttc
    15061 agaaggcggt tcacgtcctg cacctgcata ccgcaaccgg cagcgatacg acgcttacgc
    15121 gaacctttga tgatttctgg cttagcgcgc tcttttatcg tcatcgagtt gatgatggct
    15181 tccatacgca ccagcacttt atcgtccatc tgcgacttaa cattatccgg gatctgcccc
    15241 atgcccggca gcttgcccat caggctagcc atgccgccca tatttttcat ctggcgcagt
    15301 tgctcaagaa aatcgttgag atcgaagccg tcgccttttt tcagcttgct ggctaatttc
    15361 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc
    15421 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
    15481 tcgccaacac cgagaaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
    15541 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
    15601 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
    15661 gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
    15721 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
    15781 ttcagcgccg cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca
    15841 acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg ataaacgtcg
    15901 gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc gagtttacca
    15961 acgctggttg ttttaccggc accttgcagg cccgccatca gtacgaccgc aggcggttgc
    16021 gcagccaggt tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggact
    16081 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
    16141 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
    16201 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
    16261 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
    16321 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
    16381 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
    16441 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
    16501 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
    16561 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
    16621 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
    16681 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
    16741 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
    16801 ttcatgccca atgaatacat cacccatctg gaagcaacgc ctgggatgct ggtgcacatt
    16861 ggcttatcgc tcttttccta tgccacgctg ataattgccg ccctgtacgc gctacaactg
    16921 gcgtggattg attaccaact gaagaacaag aagctggcgt ttaaccaaga aatgccgcca
    16981 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctactaacg
    17041 ctcacgcttt gcactggcct gttctacatg cacaacttat ttagcatgga aaatatcgac
    17101 aaggccgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattat
    17161 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggtgc agtcatcctg
    17221 acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg
    17281 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
    17341 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
    17401 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
    17461 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
    17521 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
    17581 ctggtgtgct gacttttgtc gtactggttt ttgctgaggt attgccgaaa accattgccg
    17641 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccg ctgcaaattt
    17701 tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg tatgctgatg cgcatgatgg
    17761 gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgcactatcg
    17821 tgcacgaatc acgctcacaa atttcccgtc gcaatcagga tatgctactg tctgtgctcg
    17881 atctggaaaa gatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg
    17941 atatcaacga tgactggaaa tcgattctgc gccaactctc ccactcacct cacgggcgca
    18001 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct
    18061 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg
    18121 agatctattt tgtgccggaa ggtacgccgc tcagcacgca gttggtaaag tttcagcgca
    18181 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcagggg ctggtgacgg
    18241 ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg
    18301 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
    18361 gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
    18421 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg
    18481 agtacgatat agatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
    18541 ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
    18601 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
    18661 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
    18721 cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc
    18781 ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac
    18841 ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat ccgggttagc tttatcagcc
    18901 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
    18961 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gtcgacgcag gttttccatt
    19021 tcggctttta cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc cagctgagct
    19081 tcgagattcg caattttttc atcgcgcgga tccacctgct cagcagaagc ttctggctca
    19141 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
    19201 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
    19261 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
    19321 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
    19381 acacccacgg caccccactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
    19441 aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt
    19501 gaaaactggc acgctcgcgg agattgggca actagctgat ctcgcggtag tcgttggtgg
    19561 cgacggtaat atgctgggcg cggcacgcac actcgcccgt tacgatatta aagttattgg
    19621 aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca
    19681 gttagccgat gtgctggaag gccactacat cagcgagaaa cgttttttgc tggaagcgca
    19741 agtctgtcag caagattgcc agaaacgcat cagcaccgcg ataaatgaag tggtgcttca
    19801 tccaggcaaa gtggcgcata tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt
    19861 ttctcagcga tctgatggac taattatttc gacgccaaca ggctccaccg cctattccct
    19921 ctctgcaggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
    19981 cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg
    20041 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
    20101 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
    20161 gaaagattac agttatttca acacattaag caccaagctc ggctggtcaa aaaaattatt
    20221 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
    20281 gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg
    20341 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    20401 ccggcgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg
    20461 aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga
    20521 aagatacgcc agcggctctg cgctggctgg aagaaaatca gcttgaagac gggcatgaat
    20581 gtttgcttcg tcgagtgatc agcagcgatg gtcgctcccg tggtttcatc aacggtacag
    20641 ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc
    20701 acgctcatca attactcacc aaacctgagc accaaaaatt cctgcttgat ggctatgcca
    20761 atgaaacctc tctactgcag gaaatgaccg cacgttatca gttgtggcat caaagctgcc
    20821 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc cgcccgtgcg gaactgctgc
    20881 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gaacaaatcg
    20941 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat
    21001 tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    21061 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    21121 aagaagctac catccagatt gctgaagcca gcgatgaact gcgccactac tgcgatcgtc
    21181 tggatctcga tcccaaccga ctatttgaac ttgaacagcg catctcaaaa cagatttcgc
    21241 tggcacgtaa acatcacgtc agccctgagg cattgccaca gtattaccag tcgctactgg
    21301 aagaacagca gcaactggac gatcaggccg actcacaaga aacgcttgcg ctggcggtaa
    21361 cgaaacatca tcagcaggca ctggaaatcg cgcgcgcatt acaccaacaa cgccagcaat
    21421 atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    21481 ggcagtttac gatcgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc
    21541 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    21601 catccggtgg tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa
    21661 tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg
    21721 cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc
    21781 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    21841 gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa agcgcggtta caagagctgg
    21901 cgcgcctgct tggtggcagt gaagtcacac gtaatacact ggcgaatgcg aaagaactgc
    21961 ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    22021 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    22081 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    22141 gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt
    22201 taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt
    22261 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    22321 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    22381 cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa
    22441 cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca
    22501 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa
    22561 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    22621 tatcgcttag ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat
    22681 cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct
    22741 gcagcgtcac gcgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa
    22801 ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagac
    22861 gccagctctt taaacacgcg accaaaggcg agttcaatca acttattggt aaactcaaag
    22921 tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca
    22981 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    23041 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    23101 atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga
    23161 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    23221 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    23281 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    23341 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    23401 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    23461 caagcgcgcc cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca
    23521 aggctgggag gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct
    23581 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc
    23641 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc agcgcgaact
    23701 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    23761 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaaa aacagcacga
    23821 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcacgta tcatgaaaaa
    23881 cgcccaccgt taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc
    23941 ggcggcgcga atgaacatct tattggctat cacatccgac acaaatgttg ccatcccatt
    24001 gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata acgccattga
    24061 ggctggtcat ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg
    24121 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    24181 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    24241 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    24301 gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    24361 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac
    24421 cgaggatgta ggaatttcgg acgcgggttc aactcccgcc agctccacca cttttgatag
    24481 gactgcaatc ggacagcggc aataaaaaca gccacttacg gactctgacc agacagagcg
    24541 cagaccgaga aaagacaaaa aaatgcacgt aaaatgcacg tgcactttaa aagaacccca
    24601 gatctcacgg tctggttttt ttctgtttgt aactaatggt aacaaaaaca catcacctct
    24661 tggcgttcct tctccttgat actgtttatc catacagtta aaaataacac tgtatacaaa
    24721 cacagtatag agagattttt atgcgtattg aaatttgcat agccaaagaa aaaatcagca
    24781 aaatgccaaa aggatctgtt cctgccctac agcaagaaat gctgtgacgt gccagtaaac
    24841 gttatgacga tgtagaagtg atcatcaaat ccaccagcaa cgatggcctt tcagttactc
    24901 tcaccgccga taaagattct gcaaaaactt ttgttcagga gacgctgaaa gatacctggg
    24961 agtctgctga cgagtggttt gttcgctaat taacgagtaa aatcagtaac ggctggaaat
    25021 cattcaatac tcacactatc taaaagttca ccagccaaca gcggtaagtt ctttcatacg
    25081 acgtgccgcg gatcctatta ttctagtatg aaatccctaa ccgatttcaa attctccaga
    25141 ctgctcagat tctcttttat cccacgctgg cgctgataaa tctagtcatt gcaatcgacc
    25201 tgcgctatcg ctgccgcagt tcttccagtt tctgcatcga
//
LOCUS       DAZKGS010000062        24215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKGS010000062 DAZKGS010000000
VERSION     DAZKGS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..168)
                     /locus_tag="ACHOQY_002444"
     CDS             complement(34..168)
                     /locus_tag="ACHOQY_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678525.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            244..2805
                     /gene="mutS"
                     /locus_tag="ACHOQY_002445"
     CDS             244..2805
                     /gene="mutS"
                     /locus_tag="ACHOQY_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9678526.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2911..3567
                     /gene="pphB"
                     /locus_tag="ACHOQY_002446"
     CDS             2911..3567
                     /gene="pphB"
                     /locus_tag="ACHOQY_002446"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9678527.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(3618..4385)
                     /gene="ygbI"
                     /locus_tag="ACHOQY_002447"
     CDS             complement(3618..4385)
                     /gene="ygbI"
                     /locus_tag="ACHOQY_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP9678528.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4581..5489
                     /gene="ltnD"
                     /locus_tag="ACHOQY_002448"
     CDS             4581..5489
                     /gene="ltnD"
                     /locus_tag="ACHOQY_002448"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP9678529.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            5486..6748
                     /gene="otnK"
                     /locus_tag="ACHOQY_002449"
     CDS             5486..6748
                     /gene="otnK"
                     /locus_tag="ACHOQY_002449"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP9678530.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            6745..7383
                     /locus_tag="ACHOQY_002450"
     CDS             6745..7383
                     /locus_tag="ACHOQY_002450"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP9678531.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            7388..8164
                     /locus_tag="ACHOQY_002451"
     CDS             7388..8164
                     /locus_tag="ACHOQY_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9678532.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            8253..9617
                     /locus_tag="ACHOQY_002452"
     CDS             8253..9617
                     /locus_tag="ACHOQY_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP9678533.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(9656..9892)
                     /locus_tag="ACHOQY_002453"
     CDS             complement(9656..9892)
                     /locus_tag="ACHOQY_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP9678534.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(9903..11330)
                     /locus_tag="ACHOQY_002454"
     CDS             complement(9903..11330)
                     /locus_tag="ACHOQY_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP9678535.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(11330..11923)
                     /locus_tag="ACHOQY_002455"
     CDS             complement(11330..11923)
                     /locus_tag="ACHOQY_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP9678536.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            12070..12477
                     /locus_tag="ACHOQY_002456"
     CDS             12070..12477
                     /locus_tag="ACHOQY_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9678537.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(12598..13590)
                     /gene="rpoS"
                     /locus_tag="ACHOQY_002457"
     CDS             complement(12598..13590)
                     /gene="rpoS"
                     /locus_tag="ACHOQY_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9678538.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(13653..14747)
                     /gene="nlpD"
                     /locus_tag="ACHOQY_002458"
     CDS             complement(13653..14747)
                     /gene="nlpD"
                     /locus_tag="ACHOQY_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9678539.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(14932..15558)
                     /gene="pcm"
                     /locus_tag="ACHOQY_002459"
     CDS             complement(14932..15558)
                     /gene="pcm"
                     /locus_tag="ACHOQY_002459"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9678540.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(15552..16313)
                     /gene="surE"
                     /locus_tag="ACHOQY_002460"
     CDS             complement(15552..16313)
                     /gene="surE"
                     /locus_tag="ACHOQY_002460"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9678541.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(16294..17343)
                     /gene="truD"
                     /locus_tag="ACHOQY_002461"
     CDS             complement(16294..17343)
                     /gene="truD"
                     /locus_tag="ACHOQY_002461"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9678542.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(17340..17819)
                     /gene="ispF"
                     /locus_tag="ACHOQY_002462"
     CDS             complement(17340..17819)
                     /gene="ispF"
                     /locus_tag="ACHOQY_002462"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9678543.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(17819..18529)
                     /gene="ispD"
                     /locus_tag="ACHOQY_002463"
     CDS             complement(17819..18529)
                     /gene="ispD"
                     /locus_tag="ACHOQY_002463"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9678544.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(18548..18859)
                     /gene="ftsB"
                     /locus_tag="ACHOQY_002464"
     CDS             complement(18548..18859)
                     /gene="ftsB"
                     /locus_tag="ACHOQY_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9678545.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(19053..19376)
                     /locus_tag="ACHOQY_002465"
     CDS             complement(19053..19376)
                     /locus_tag="ACHOQY_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9678546.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(19426..20031)
                     /gene="cysC"
                     /locus_tag="ACHOQY_002466"
     CDS             complement(19426..20031)
                     /gene="cysC"
                     /locus_tag="ACHOQY_002466"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9678547.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(20031..21458)
                     /gene="cysN"
                     /locus_tag="ACHOQY_002467"
     CDS             complement(20031..21458)
                     /gene="cysN"
                     /locus_tag="ACHOQY_002467"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9678548.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(21460..22368)
                     /gene="cysD"
                     /locus_tag="ACHOQY_002468"
     CDS             complement(21460..22368)
                     /gene="cysD"
                     /locus_tag="ACHOQY_002468"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9678549.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            22620..23657
                     /gene="iap"
                     /locus_tag="ACHOQY_002469"
     CDS             22620..23657
                     /gene="iap"
                     /locus_tag="ACHOQY_002469"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9678550.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   23739..24194
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=23800..23828
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT         5679 a         6512 c         6257 g         5767 t
ORIGIN      
        1 ttgaaagggg actatggctc tgaaggagga ggtttattca accgacgcaa ttttgcgtac
       61 ttgcttcata agcatcacgc aaaagctgca aaacagcatc tttcccggaa ccagcatcaa
      121 gaactcgcag ttcgcttctt cccctgaaat gattaactcc ggtatcatgt gcgccttatg
      181 tgattacaac gaaaataaaa accatcacac cccatttaat atcagggaac cggacataac
      241 cccatgagtg caatagaaaa tttcgacgcc catacgccca tgatgcagca gtatctcagg
      301 ctgaaagccc agcatcccga gatcctgctg ttttaccgga tgggtgattt ttatgaactg
      361 ttttatgacg acgcaaaacg cgcgtcgcaa ctgctggata tttcactgac caaacgcggt
      421 gcttcggcgg gagagccgat cccgatggcg gggattccct accatgcggt ggaaaactat
      481 ctcgccaaac tggtgaatca gggagagtcc gttgccatct gcgaacaaat tggcgatccg
      541 gcgaccagca aaggtccggt tgagcgcaaa gttgtgcgta tcgttacgcc aggcaccatc
      601 agcgatgaag ccctgttaca ggagcgtcag gacaacctgc tggcggctat ctggcaggac
      661 agcaaaggtt tcggctacgc gacgctggat atcagctccg ggcgttttcg cctgagcgaa
      721 ccggccgacc gcgaaacgat ggcggcagag ctgcaacgca ctaatccggc ggaactgctg
      781 tatgcagaag atttcgccga gatgtcgctg attgaaggcc gtcgcggcct gcgccgtcgc
      841 ccgctgtggg agtttgaaat cgacaccgcg cgccagcagt tgaatctgca atttggcacc
      901 cgcgatctgg tcggttttgg cgtcgagaac gcgccgcgcg gactttgtgc tgccggttgt
      961 ctgttgcagt atgcgaaaga tacccaacgc acgaccctgc cgcatattcg ttctatcacc
     1021 atggaacgtg agcaggacag catcattatg gatgccgcga cacgtcgtaa cctggaaatt
     1081 actcagaacc tggcgggcgg tgcggaaaat acgctggctt ctgtgctcga ctgcaccgtc
     1141 acgccgatgg gtagtcgtat gctgaaacgc tggctgcata tgccagtgcg cgatacccgc
     1201 gtgttgcttg agcgccagca aactattggc gcattgcagg atttcaccgc cgagttgcag
     1261 ccggtactgc gtcaggtcgg cgacctggaa cgtattctgg cacgtctggc tttacgaact
     1321 gctcgcccac gcgatctggc ccgtatgcgc cacgctttcc agcaactgcc ggagctgcgt
     1381 gcgcagttag aaactgtcga tagtgcaccg gtacaggcgc tacgtgagaa gatgggcgag
     1441 tttgccgagc tgcgcgatct gctggagcga gcaatcatcg acacaccgcc agtgctggta
     1501 cgcgacggtg gtgttatcgc atcgggctat aacgaagagc tggatgagtg gcgcgcgctg
     1561 gctgacggcg cgaccgatta tctggagcgt ctggaagtcc gcgagcgtga acgtaccggc
     1621 ctggacacgc tgaaagttgg ctttaatgcg gtgcacggct actacattca aatcagccgt
     1681 gggcaaagcc atctggcacc catcaactac atgcgtcgcc agacgctgaa aaacgccgag
     1741 cgctacatca ttccagagct aaaagagtac gaagataaag ttctcacctc aaaaggcaaa
     1801 gcactggcac tggaaaaaca gctttatgaa gagctgttcg acctgctgtt gccgcatctg
     1861 gaagcgttgc aacagagcgc gagcgcgctg gcggaactcg acgtgctggt taacctggcg
     1921 gaacgggcct ataccctgaa ctacacctgc ccgaccttca ttgataaacc gggcattcgc
     1981 attaccgaag gtcgccatcc ggtagttgaa caagtactga atgagccatt tatcgctaac
     2041 ccgctgaatc tgtcgccgca gcgccgtatg ttgattatta ccggcccgaa catgggcggt
     2101 aaaagtacct atatgcgcca gaccgcactg attgcgctga tggcctacat cggcagctat
     2161 gtaccggcac aaaaagtcga gattggacct atcgatcgca tctttacccg cgtaggcgcg
     2221 gcagatgacc tggcttccgg gcgctcaacc tttatggtgg agatgactga aaccgccaat
     2281 attttacata acgccaccga atacagtctg gtgttaatgg atgagatcgg gcgtggaacg
     2341 tccacctacg atggtctgtc gctggcgtgg gcgtgcgcag aaaatctggc gaataagatt
     2401 aaggcattga cgctgtttgc cacccactat ttcgagctga cccagctacc ggagaaaatg
     2461 gaaggcgtcg ccaacgtgca tctcgatgcg ctggagcacg gagacaccat tgcctttatg
     2521 catagcgtgc aggatggcgc ggcgagcaaa agctacggcc tggcggttgc agctctggca
     2581 ggcgtgccaa aagaggtgat taagcgcgca cggcaaaaac tgcgtgagct ggaaagcatt
     2641 tcgccgaacg ccgccgctac gcaagtggat ggtacgcaaa tgtctttgct gtcagtgcca
     2701 gaagaaactt cgcctgcggt cgaagcactg gaaaatctcg acccggattc actgactccg
     2761 cgtcaggcgc tggagtggat ttatcgcttg aagagtctgg tgtaataaca attcccgata
     2821 gtcttttgct atcgggaata ttaacgacaa ctgacgaatc aaataaaaat accctgtata
     2881 ataggaaagc ttattttaca gggtaaaacc atgccatcta cacgctatca aaaaatcaat
     2941 acccatcact atcgccatat atgggtcgtt ggtgatattc atggtgaata tcagttatta
     3001 caatcccgct tacatcaact ctcttttttc cccgaaaccg acttacttat ttctgtcggc
     3061 gataatattg atcgtgggcc ggagagtctt gacgtcctgc gcctgctaaa ccagccctgg
     3121 tttatctccg ttaaaggcaa ccacgaagca atggcgctgg atgcgttcgc gaccggcgat
     3181 ggcaatatgt ggcttgccag cggtggtgac tggtttttcg atttaaatga ttcagagcaa
     3241 aaagaagcta cagatctgtt gctgaaattc catcaccttc cacatattat tgaaatcact
     3301 aacgacaaca taaaatatgt catcgcacat gcagattatc cggggagtga atatctcttt
     3361 ggtaaagaaa tagcggagag cgaattactc tggcctgttg atcgtgtgca gaaatcgctt
     3421 aatggcgagt tacaacaaat aaacggcgct gattatttta tatttggaca tatgatgttt
     3481 gataacattc agacgttcgc taaccagatt tatattgata ccggcacgcc gaaaagcggg
     3541 cggctgtcat tttataaaat aaagtagtct catgcttctt ctgtgaagca tgagtaacca
     3601 aatgttattg caggccatta ttcatttttc gctaccagca aagagagatc ctgcttcgcc
     3661 agcgcgcgac tggcactctc cggcaaaccg tcgtctgtaa taatctgatc aaactcgctt
     3721 aatggtaacg ccagccatgt cgccacctga ccatatttcg tcgcatcaca gaccaaaact
     3781 cgctggcggc tggcactggc aatcgcccgt ttcaccgtga ctttatcttc cgctggcgta
     3841 gaaatccccc gcacactcca tgacgatgca gaaataaaag cctgatcaat catcaggctg
     3901 cgcagcatgg tcgcagcggc ttccccgaca caggaacggt tttcccgaca cactgcaccg
     3961 ccagtgtgaa taattgtgca attactgttg tcgagcaagt agttcgcaat aacgaaatcg
     4021 tttgtgacca cagtcagtga ctccatgtga atcagatgct gtgctatcgc taacgtggtc
     4081 gttcccgcat ccagatagat acaacttccc ggctgaacaa gacttgccgc cagcttgcca
     4141 atagccgctt tttgcgtcat tgccagcgca gtttttacct gatgagaagg ttcatgcgcc
     4201 acgcgtcccg gagactggac gcctccggac accagcacaa cggctccctg ctgctccagt
     4261 ttttgtaaat cccgacgaat ggtcatatgt gacacattca ttctgtccgt tagttcagca
     4321 atactgacaa tgcctttttc agctaccatc tcaaggatga tttggcgacg ctctacgggt
     4381 atcaactttt gctccttcct ttgtcctgct gacattctac gctatttgcc tgcgaaacgt
     4441 gcgcggcgca actaacgctt agttcacata aaataacaca caatgttaat ttatgtgaat
     4501 cagctcacca tatcgttatc ttccagcgct tatattcaca atatcaaaca aaatatcact
     4561 taaattaaca aggggagcag atgaaaacgg gatctgagtt tcatgtcggt atcgttggct
     4621 tagggtcaat gggaatggga gcagcactgt catgtgtccg cgcaggtctt tctacctggg
     4681 gcgcagacct gaacagcaat gcctgcgcta cgttgaaaga ggcaggtgct tgcggggttt
     4741 ctgataacgc cgcgacgttt gccgaaaaac tggacgctct gctggtgctg gtggtcaatg
     4801 cggcccaggt taaacaggtg ctgtttggtg aaacaggcgt tgcacaacat ctgaaacccg
     4861 gtacggcagt aatggtttct tccactatcg ctagtgctga tgcgcaagaa attgctaccg
     4921 ctctggctgg attcgatctg gaaatgctgg atgcgccagt ttctggtggt gcagtaaaag
     4981 ccgctaacgg tgaaatgact gtcatggcct ccggtagcga tattgccttt gaacgactgg
     5041 cacccgtgct ggaagccgtt gccggaaaag tttatcgcat aggtgcagaa ccgggactag
     5101 gttcgaccgt aaaaattatt caccagttgt tagcgggcgt acatattgct gccggagccg
     5161 aagcgatggc acttgcagcc cgtgcgggga tcccgctgga tgtgatgtat gacgtcgtga
     5221 ccaatgccgc cggaaattcc tggatgttcg aaaaccggat gcgtcatgtg gtggatggcg
     5281 attacacccc gcattcagcc gtcgatattt ttgttaagga tcttggtctg gttgccgata
     5341 cagccaaagc cctgcacttc ccgctgccat tggcctcaac agcattgaat atgttcacca
     5401 gcgccagtaa cgcaggttac gggaaagaag acgatagcgc agttatcaag attttctctg
     5461 gcatcactct accgggagcg aaatcatgat caagattggc gttatcgccg atgattttac
     5521 cggcgcgacg gatatcgcca gttttctggt ggaaaacggt ctaccaacgg tacaaattaa
     5581 cggtgttcca acaggtaaaa tgccggaagc aatcgacgca ctggtgatca gcctgaaaac
     5641 gcgctcctgt ccggtggttg aagccacaca gcaatcgctg gcggctctga gctggttgca
     5701 acagcaaggt tgcaaacaga tctatttcaa atactgctct actttcgaca gtacggcgaa
     5761 aggtaatatc ggcccggtta ccgatgcatt aatggatgct ctcgacacgc cgtttacggt
     5821 cttctctccg gccctgccgg tcaacggacg tacggtttat caggggtatt tgttcgtaat
     5881 gaatcaactg ctggccgaat ccgggatgcg ccatcatccg gtaaatccca tgaccgacag
     5941 ctatcttccc cgtctggttg aagcgcaatc cacagggcgc tgcggcgtcg tttcggcaca
     6001 tgttttcgaa caaggtgtgg atgccgttcg tcaagagctg gctcgcttac agcaagaggg
     6061 ctaccgctac gcggtgcttg atgcgctgac cgaacaccat ctggaaattc agggagaagc
     6121 cttgcgcgat gccccactgg taacgggcgg ttctggtctg gcgattggcc tggcccggca
     6181 gtgggcgcaa gaaaacggta accaggctcg cgaagcaggg catccgctcg ctgggcgcgg
     6241 cgtagtgctc tccggttcat gctctcaaat gaccaaccgc caggtggcac attaccgtca
     6301 aattgcacca gcccgtgaag ttgatgtggc acgctgcctc tcaaccgaaa ctctggccgc
     6361 ttatgcacac gaactggcag agtgggttct gggccaggaa agtgtacttg ctccactggt
     6421 ttttgccacc gccagcactg acgcattggc agcaattcaa cagcaatacg gtgcacaaaa
     6481 agccagtcag gcagtagaaa ctctattttc tcaactagcg gcgcggttag cagcggaagg
     6541 cgtgacacgc tttattgtcg caggcggtga gacctccggc gtagtcacac agagcctggg
     6601 cataaaaggg tttcatattg gcccaaccat ttcccccggc gtgccgtggg taaacgcact
     6661 ggataagcct gtctcactcg cccttaaatc tggcaacttc ggtgatgaag cctttttttc
     6721 acgagcccaa agagagtttt tatcatgagc gatttcgcaa aagtagagca gtctttgcga
     6781 gaggagatga cgcggattgc cagttcattc tttcagcgcg gctatgcaac cggttcggct
     6841 ggcaatctgt cgctgctttt acctgacggg aatttactgg cgacaccgac aggttcatgc
     6901 ctgggcaatc tcgatccgca gcggctttcc aaagtcgccg cggatggcga atggttaagt
     6961 ggtgacaaac cctcgaaaga ggtgctcttt catctggcgc tgtatcgcaa caatccgcgc
     7021 tgtaaagcgg tggtgcattt gcacagcaca tggtcgacgg cgctttcctg cctgcaaggg
     7081 ctggacagca gcaacgttat tcgtccgttc acaccatacg tggtgatgcg gatgggaaat
     7141 gtcccgctgg tgccttatta ccgaccgggc gataaacgca tcgcacagga tctggcggaa
     7201 ctggcagcag acaatcaggc ttttttactg gcaaatcatg gcccagtggt ttgcggtgaa
     7261 agcctgcaag aagccgccaa caatatggaa gagctggagg aaacggcaaa gctgattttt
     7321 attctcggtg accgcccgat ccgttatctg accgcaggtg aaattgcgga attaaggagt
     7381 taatgcaatg cctcgttttg cagctaattt atccatgatg ttcaccgaag tgccttttat
     7441 tgaacgcttc gccgcagcgc gaaaagccgg gttcgatgct gtggaatttc tgtttcccta
     7501 tgactactcc accctgcaaa tccaaaagca actggagcaa aaccatctga cactggcgct
     7561 gttcaatacc gctcccggag atattaatgc gggggaatgg ggattatccg cccttcccgg
     7621 acgtgagcac gaagcacacg cagacatcga cctggcactg gaatatgcat tggcgctcaa
     7681 ttgtgaacaa gtccatgtga tggcaggcgt cgtgcccgct ggcgaagacg ctgaacggta
     7741 ccgggcagta tttatcgata atctccgcta cgctgccgac cgctttgcgc tacacggcaa
     7801 gcgaatatta gttgaagcac tcagccccga cgttaagcct cattatctct tctccagcca
     7861 gtatcaggca ctggctattg ttgaagaggt tgcgcgagat aacgtgttta ttcaactaga
     7921 tacttttcat gcgcaaaaag tagatggcaa cctgacgcat ttaatccgcg actacgctgg
     7981 gaaatatgcg cacgtacaaa ttgccggact acctgatcga catgaaccgg acgatggaga
     8041 aatcaactat ccgtggctgt tccgcctgtt cgatgaggtg ggatatcagg gctggatcgg
     8101 ttgtgaatat aaacctcgtg gcctcaccga agaagggctt ggctggtttg acgcctggcg
     8161 ctaattcgtt aaatccctaa ttacaacgta cccatacatc cccccatcta cggatggggt
     8221 aggttaacta taactttcag acagggtttc ccatgtccac aattacattg ttatgcattg
     8281 cgttagctgg cgtaatcatg ctgttgctgc tggtcatcaa ggcaaaggta caaccattcg
     8341 ttgctctgct cctcgtcagc ctgttagtcg cacttgcggc gggtataccg gcgggcgaag
     8401 tgggtaaagt gatgatcgcc gggatgggcg gtgtgcttgg ttccgtcact attattattg
     8461 gtctgggcgc tatgctgggg cgtatgatcg aacactctgg tggtgcagag tcactggcta
     8521 attatttcag tcgcaagtta ggtgacaaac gaactatcgc tgcgctgact ctggcagcgt
     8581 tcttcctcgg tattcccgtc ttctttgatg tcggctttat tattcttgcg ccaatcattt
     8641 acggttttgc caaggttgcc aaaatatcgc cactcaaatt tggcctgcct gtcgctggaa
     8701 tcatgctaac tgttcatgtg gcggtaccgc cgcatccagg ccctgtcgcc gcagcggggt
     8761 tactccacgc agacatcggc tggctaacca tcatcggtat tgcgatttct attcccgtag
     8821 ggattgttgg ctactttgca gcgaaaataa tcaatacgcg ccaatatgcg atgtcggtgg
     8881 aagtactgga acagatgcaa ctggctccgg ccagtgagga aggcgcaaca aaattaagcg
     8941 ataaaataaa tccaccgggc gtcgcgctgg tcacctcgct aattgttatt cctatcgcga
     9001 ttatcatggc gggtacggtt tccgcaacac tgatgccgcc ttcgcatccc ctgcttggta
     9061 cgctacagct gatcggctca ccaatggtag ctctaatgat tgcgctggtg ctggcattct
     9121 ggttattggc tttacgtcgc ggctggagct tacaacacac cagcgacatt atgggctcag
     9181 cccttcccac tgcggcggta gtgattttgg ttactggtgc tggaggggta tttggcaaag
     9241 tgctggtgga atcgggcgtc ggcaaagccc ttgccaatat gctacaaatg attgacctgc
     9301 ctctgttacc agccgcattt attatttcac tggctctgcg tgcatcgcag gggtcagcaa
     9361 ccgtagcaat cctgacaacc ggcgggttac tctcagaagc ggtgatggga ttgaatccga
     9421 ttcagtgcgt attggtgacg ctggcagcct gctttggtgg gcttggcgcg tcacatatta
     9481 atgactcagg gttctggatt gtgaccaaat atctggggtt gtcggtagca gacggtctga
     9541 aaacctggac agtgttaacg accattctcg gttttaccgg attcttaatt acctggtgcg
     9601 tatgggcggt aatttgacct taataaaaag gtccgatggg catcggacct tttatttaac
     9661 gcttaccttc caccagcagc ggcgggatgc tcggcaccat tggcgcgtca tcaatatctt
     9721 tctgcgtcat gcggaacgct tcgggataat gttcgcggct ggtacggcgc agcgattcgg
     9781 tatcgcgcca ggtataaagg caatgctggc actgatatac cgtccagaca tctttcaccg
     9841 gcgatttcgc catcacttca atctgttcat cggcacaacg tggacaaatc atcttcttct
     9901 ccttatttac gtgcagccag catagcggtc agtttttcag cccaggcttt ggtttccggt
     9961 aaatccacca ccggttggct gtagtgacca cggttgtccg gggcgacagg cgtagtggcg
    10021 tcgataatca gcttgtcggt gatccccgca gggcttgagc ctggatcgag ttccagcacg
    10081 gacatattcg gcaactgcac caaatcccct gccgggttca ctttcgagga gagcgcccac
    10141 atcacctgcg gcaggttgaa cgggtcaacg tcttcatcga ccataatcac catcttcacg
    10201 tagcccagac catgcggcgt ggtcattgcg cgcaggccca ccgcgcgggc aaagccgccg
    10261 tagcgttttt tggtggagat aatcgccagc aggccatggg tgtacatggc gtttaccgcc
    10321 tgcacttcag ggaactcggc tttcagctgc tgatacagcg gcacgcaggt ggctggcccc
    10381 atcaggtagt cgatttcggt ccacggcata ccgaggtaca gcgattcgaa aatcggcctg
    10441 gtgcggtaag agactttatc gatgcgcacc acggtcatgt tacgcccgcc ggagtagtgc
    10501 ccggtaaact caccgaacgg cccttcgatt tcgcgtttac ggctttcgat gaccccttcg
    10561 aggatcactt ctgaacccca cggcacatca aaaccggtca acggggcggt ggcgatcggg
    10621 tacgggcttt cacgcagcgc gcctgccatt tcgtactcgg actgatcata tttcagcggc
    10681 gtggccccca tcagcgtgat gatcggatcg ttaccgagcg tgatcgcaat cggcagatct
    10741 tcaccgcgct cttctgcttt atgcagatgc agggcgatat cgtgcatcgg caccggttgc
    10801 aggccgagct tacgcttgcc cttcacttcc atgcggtaga tgccgacgtt ctgcttgccg
    10861 aagttatccg ggtcgagcgg atcgcgggaa accacgcacg ctttgtcgag atagaaaccg
    10921 ccatcgccat cgtttaaacg aaacagcggc aggatatcga acaggttgat ctcgtcgcca
    10981 tcaacggtgt tctgcgccca ggctggattg gcgcggcgct ccggggcaat cgggaagtta
    11041 tcccagcggc ggataaactc atcaatctgc tttttaaccg gggcatttgg cggcaggccg
    11101 agggaaatcg cgtggttctg ccaggaaccg atggtgttca tcgccacgcg ggcatcggta
    11161 aagccgcgaa tattatcaaa ccacagcgcg ggcgcgccgt cgccgatacg cccggtggcg
    11221 ttagctgctg ctgccagatc cggctcggcg ttcacttctt cgctgatttt cagtaactgg
    11281 ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat caaatgccat tattcattct
    11341 cctgagaaaa attccgggcc tgcggcaatc cttgccagcg cctggcgtgg ggatgttcga
    11401 ggccaaattg atccagcacg cgggctacca catggtggac aatgtcatct accgtttcgg
    11461 gatggttata aaaggcaggc atcggcggca ccatcgccac gcccatgcgt gaaagtgcga
    11521 gcatattttc gagatggatg gtgctaagcg gcatttcacg cggcaccagc accagtttgc
    11581 ggccttcttt gagcacgacg tccgccgcgc gccctaccag gccatcggcg taaccagcgc
    11641 ggataccggc gagcgttttc atactgcacg gaataacgat catgccgtcg gtacgaaaag
    11701 aaccggatga gatgatcgcc gcctgatccg ccgggttatg gctgaagtct gcgagggcag
    11761 caacatcgcg agcgctgtaa ggcgtttcca gttcaatggt ggttttcgcc cacttcgaca
    11821 tcaccagatg agtctcgaca ttcggcatct cccgcagcgc ttgcagtaat gccacaccaa
    11881 gaggcgcacc ggtagcccct gtcatcccga cgatcagttt cattgcttat cctcttaatt
    11941 gttcgtatac gaacgttatt attaacatac tccttcattt ccacagcggc aaggattgaa
    12001 aagtaacaac atcttcttcg tcactaaaaa accgctatga tagcgacaga gaattagccc
    12061 ggagtttaca tggcgttacg aaataaagcg ttccatcagt tacgacagct ttttcagcag
    12121 cacacggctc gctggcagca cgagttacct gacctgacca aaccacagta cgcggtgatg
    12181 cgcgccattg ctgataaacc tggtatcgaa caggtggcac tgatagaagc agctgtcagc
    12241 accaaagcaa cgttagcaga aatgttggca agaatggaaa atcgtggctt agtcagacgt
    12301 gaacatgacc ccgctgataa gcggcgacgc tttgtctggt tgactgccga aggcgaaaag
    12361 atacttgctg cggcgatacc cattggtgac agcgtggatg aggaattttt ggggcgtttg
    12421 agtggagaag agcaggagct gtttatgcag ctggtgcgca agatgatgaa cgcatagggg
    12481 gcaaatttcc ggatgcgacg ctatcgcgtc ttatccagac tacaaataca ccgactcacc
    12541 aaccgaaaga aaaaaggcca gcctcgcttg agactggcct ttctgacaga tgcttactta
    12601 ctcgcggaac agcgcttcga tattcagccc ctgcgtttgc aggatttcgc gcaaacggcg
    12661 caggccttca acctgaatct ggcgaacacg ttcacgggtg aggccaattt cacgacctac
    12721 atcttccagt gttgccgctt cgtaccccag caaaccgaat cgacgtgcca gcacttcacg
    12781 ctgtttggcg ttcagctcga acagccattt gacgatgctc tgcttcatat cgtcatcttg
    12841 cgtggtatct tccggaccgt tctctttttc atcggccagg atgtccagca acgctttttc
    12901 ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc tcgttaagac gaagcatacg
    12961 gctgacgtca tcaactggct tatccagttg ctctgcgatc tcttccgcac ttggttcatg
    13021 gtccagctta tgggacaact cacgtgcggt tcgcaggtaa acgttcagct cctttacgat
    13081 gtgaatcggc aaacgaatag tacgggtttg gttcataatc gcccgttcaa tcgtctggcg
    13141 aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt tccgggtcaa acttctctac
    13201 cgcgcggatc agccccaggt tgccctcttc gataaggtcc agcaacgcca gaccacgatt
    13261 gccataacgg cgggcaattt ttaccaccag acgcaagtta ctctcgatca tccggcggcg
    13321 agaggcgaca tctccacgca gtgcgcgacg cgcaaaataa acttcttctt cggccgttaa
    13381 cagtggtgaa taaccaatct caccaaggta aagctgagtc gcgtccaaca cacgctgtgt
    13441 ggctccctgc gataacagtt cctcttcggc caaatcgtta tcactgggtt cctcttctac
    13501 taaggccttt tcgtcaaaaa cctcaactcc gttctcatca aattccgcat cttcatttaa
    13561 atcatgaact ttcagcgtat tctgactcat aaggtggctc ctacccgtga tcccttgacg
    13621 gaacattcaa gcaaaagcct ggttccgccg atttatcgct gcggcaaata acgcagcggg
    13681 tttacggatt tccccttgta acgaatttca aaatgcaagc gtgttgaact ggttccggtg
    13741 ctacccatgg ttgctatttt ttgccccgcc ttcacttctt gttgttcccg gaccagcatt
    13801 gtgtcgttat gggcgtaggc actcaggtaa tcatcattat gtttgatgat aatcagatta
    13861 ccgtagccgc gcagcgcgtt accggcataa acaacgcggc catctgcggt cgcgataatt
    13921 gcctgtcctt tgctgcctgc gatatcaatc cccttgttgc ccccctcaga agcgccaaag
    13981 gtttcgatca ctttgccctc agtcggccag cgccaggtgg agataggcgt actggttgat
    14041 gtactgctga cagttggctc ggttgtgctt gctgttggta ccgttacagg cgctgtgacc
    14101 gtggtcgcag ctggcttgtt gttcggcaac attttgttag cactctgttc acccgaagat
    14161 tcagaatacg taattgtcgg ttgcgacgca acagcaacgg tggaattttg tgcaggcttg
    14221 atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg catttccgcc agtgattggc
    14281 gtaccggaag cattacccac ctgcaaggtc tgaccaacgt tcagcgcgta tggtgcctga
    14341 atattgttgc gctgagcaag gtcacggaaa tcgttgccag taatccaggc gatatagaaa
    14401 agtgtgtcgc cttttttcac ggtataggta ctgccgctat aactgccttt cggaatgttc
    14461 ccatactgac ggttatagac gatgcgtccg ttttccatct gtaccggctg ctgagctact
    14521 ggctgcactg gctggatttg cggttgttga gtagcctgaa tttgtggctg ctgtaccggc
    14581 tgaatttgcg gttgctgggc tgtagacgtc gtccccattt tcggcggcgg cgtaatcaac
    14641 ataccagaat tggtatttgc aggcgcattg ccattaacgg agctgaccgg tgccggtgga
    14701 tttgaagtgt cagaacagcc tgccagccat agcgaaacca gtgacaaagc cgcaatgcgg
    14761 cgaacggtga attttgggct tcccgcgctc atttatcccc caggaaaaat tggttaataa
    14821 ccagtgacat aattaccgtg caaggcaccc tactgaacac tggaaaagat gttcacgata
    14881 cgctgacctg cggcaaaata accaggaaaa atccaggtat ttcctcacgt tttaagccag
    14941 ctcacccttc actaaaggta caaagcgcac ggcctccacg gtatcgataa taaattcgcc
    15001 tccccgacga cgcacccgtt tcaaatactg gtgctcctcc cctacgggta agacgagaat
    15061 cccgccttcg tccagctgcg tcattagcgc agttggaatt tccggcggtg ccgccgtaac
    15121 aatgatagcg tcaaacggcg cgcgtgcctg ccaaccttgc catccatcgc catgacgggt
    15181 tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc gcctgccact gcaaaccttt
    15241 aatccgttcg accgagcaaa catgctggac aagatgcgcc aggattgccg tttgatatcc
    15301 cgaaccagtg ccaatttcca gcacccgcga ctgcggcgtc agctcgagta attcggtcat
    15361 tcgcgccacc atatacggct gcgaaattgt ctgcccctga cctatcggca aggcgatatt
    15421 gtcccaggct ttttgttcaa acgcttcatc aacgaatttt tcacgcggca cggcggcaag
    15481 tgcattcagc acctgctcat cctgaatacc ttgcgcacgt aattgatcca gaagtgcttg
    15541 tacgcgtctg cttaccattg cgtgccaact cccacgctgt ttaaccagtc tgaaaccaca
    15601 tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg tgatggagac atagccctca
    15661 tctaccgcag caaaatcggt ccccggaccg gcatcacatt taccgcccgg cgggccaatc
    15721 cagtacagcg tattgccgcg cggatcttgc tgcgggatca cctgatctgc cggatgtcgt
    15781 gtaccgcagc gcgtcacgcg aatacctttg atttgatcca agggtaaatc cggaacgtta
    15841 atattaagaa tacgcccggt gcgcaacggc tctttacaca gtgcgcgcaa aattgaacag
    15901 gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa gcgagacggc aagcgccgga
    15961 aaacctaaat gacggccttc catcgcggcg gctaccgtac cggaataaat aacatcatcc
    16021 cccagattcg gcccggcgtt aattccggac acaacaatgt ccgggcgcgg acgcatcaga
    16081 gcattcacgc caagatagac gcaatcggtc ggggttccca tttgcacagc aatatcacca
    16141 ttttcaaagg taaacgtgcg cagggaggat tccagtgtca gagaatttga agcgccgctg
    16201 cggttacgat cgggggcgac cacctgaacg tcagcaaact cacgcaaggc tttcgccagc
    16261 gtttgtatac cgggtgcatg taccccgtca tcattactca gcaatatgcg cataatcacc
    16321 tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa ctacccgccg gaagccagaa
    16381 acggatctct acggtgacgt catcccacca attccagctt aattgttgcg gatacagcag
    16441 catcgctctg cgcgcggctt caactttttc gcgcaccagt aaagcttgta attcagtttc
    16501 tgcggcgaca gctgcttgtt cgaatgccag cgcttcacgc tgagttcccc attcgccact
    16561 gcccggcaat gcggcggtta tcatcagctc tttatcgtta acgcgacgct gtaattccgc
    16621 cagttcttcg gtggttgcga caaaccagct accacgtccg gctaattgta gcgcatcgcc
    16681 gtcaacaact tgattaacgt ctgctttttt gaggcgctca gcaacaatct gattaaacaa
    16741 cgcactgcgg gctgccgaca accaaaaact ccgtttattg cgatcgcgca ccggagtatt
    16801 ggtttgcgcc cagcgctgcg ccccctgcaa gttgctaccg ccaatcccaa aacgttgggc
    16861 accgaagtag ttcggtacac cttttacgca aatatcgttc agacgttgtt caacgtcatc
    16921 gcgattgctc acttcgcgca gaaccagggt aaaggcgttt cctttcagcg cccctaaacg
    16981 cagcttgcgc ttgtgccgcg catactccag cacctggcag ccttccagtt gaaaggcgct
    17041 cagatcgggc atttccttgc ccggcacgcg agcgcataac cactgttccg taacagcatg
    17101 tttgtctttt tgcccagcga agctgacttc acgggcatga attttcagga atttcgccag
    17161 tgcatccgcc acaaaacggg tattgcagcc gtttttgagg attctaacca gaatatgctc
    17221 accttcacca tcaggctcaa agcccaaatc ttccaccacc acaaagtctt ccggattggc
    17281 tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa gtgagattat caaactcaat
    17341 cattttgttg ccttaatgag tagcgccacc gcttcacagg caatcccttc cccacgtccg
    17401 gtaaatccca gtttttccgt agtagtggct ttcacgttaa catcatccat atggcagccg
    17461 agatcttcgg caataaacac gcgcatttgt ggaatgtgcg gcaacatctt cggtgcctga
    17521 gcgatgatag tgacatcgac gttgccaagg gtataaccct tcgcctgaat acgacgccag
    17581 gcttcgcgta gcagctcgcg gctatcggca cctttaaatg ccggatcggt atccgggaac
    17641 agcttgccga tatcccccag cgccgccgcg ccaagcaatg catcggtcaa cgcatggagc
    17701 gccacgtcgc catcagaatg cgccagcaat cctttttcgt aaggaatgcg tacgccacca
    17761 atgataattg ggccttcacc gccaaaggca tgtacgtcaa aaccgtgtcc aattcgcatt
    17821 atgtattctc ctgatggatg gttcgggtga ggtaaaactc ggccagtgcc aaatcttccg
    17881 ggcgcgtgac tttaatgtta tccgcacggc cttcgaccaa ctgaggatgg aatccgcaat
    17941 attccagcgc cgaggcttcg tcggtaatag tcgcgccttc atttagagcg cgcgtcagac
    18001 agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag cgcgtgccat aagccgttgc
    18061 gatcaacggt atgagcaatg gcatttttgc ccggttcggc acgtttcata gtatcgcgca
    18121 ctggtgcggc gaggatcccc cccgtgcggc tggtttcgct caacgccaac aatcgcgcga
    18181 ggtcatcctg atgcaaacaa ggacgagcgg cgtcatgcac caatacccac tgcgcgtcgc
    18241 cagcggcttt cagacctgcc agcacggaat cggcacgctc atcaccgcca tctacaacgg
    18301 tgatttgcgg atgattcgcc agaggaagtt gtgcaaaacg gctatcgcca ggacttatgg
    18361 caatgacgac tcgtttcacc cggggatgcg ccagcagcgc atgcaccgag tgttcaagaa
    18421 tggtttgatt accgattgag agatattgct taggacattc cgtttgcatt cgacggccaa
    18481 atccggccgc cggaaccacg gcgcaaacat ccaaatgagt ggttgccatg ttaattcccg
    18541 ggctgattta tcgattgttt tgccccgcag actgtgcgcg cttcgacgcg tcaggcacca
    18601 gacgataaaa agtttcgccc ggcctggtca tgctgagttc attacgcgca cgctcttcga
    18661 gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa aagttgatcg tttcgcgctt
    18721 taagtttcgc gtttgtagct tgctgtgccg ccacatcatc attgacgcgg gtatagtcat
    18781 gtataccgtt cttaccgaac cacagcgaat actgtagcca gaccagaata gccagcaaca
    18841 gcagcgttag tttacccatc ctgccccctg aaaaacggca tcatcatccc atgcatccga
    18901 agacgactct acatcctctg ttggggatac cgcgacaacg cgggcaaatg taccacattt
    18961 gtccattgtt acgtataccc agggcgtgca gaacataatc tcattattag ttacggtttg
    19021 aattatgaac agaggagacg ggaaagtaca aattagccca gtagccacat aaacagtgcg
    19081 ccaaacataa tgcctactgt caccagggtg aaaacaatac tgtagcgtag ctttccgtcc
    19141 atcaatgaat gcagcgcaat ccccaccact accgcgacgg gcatcagcgc cagaaagaaa
    19201 ggccaggtgt agataaagaa gaacagcgtg ttagagccat aaatcaacat cggcatcgcc
    19261 agcgcaaata accaggagat aaaaccgacc acggcaccag gcagtgacca tgtggtttct
    19321 tcatcctcag taaggctgtc gttatttgtt agtgtaatgt tatggctatt acgcatattt
    19381 gatcctgtta ctttgacgaa ccgggcatgg aaacccggtg gtgtctcagg atctgataat
    19441 atcgttctgt ctcaacagat ctaataattg ctgtaccaaa tttgttacta attgttcacc
    19501 attgagatga atttctgccg attcaggcgc ttcgtaaacg gaatctattc ccgtaaagtt
    19561 gcgcagttca ccggcacgcg ctttcttata taagcctttt ggatcgcggg cttcgcaaat
    19621 cgccagcggc gtatcgacaa acacttcgat aaagcgccct tctcctacgc gttcgcgaac
    19681 catctggcgt tcggcgcggt gtggcgagat aaatgcggtc agcactacca gtccggcttc
    19741 aaccatcaaa ttcgccactt caccgacgcg acggatattc tctttacgat cggcatcgct
    19801 aaaaccgaga tcgctgcata atccgtggcg aacattatcg ccatccagca gatacgtact
    19861 gacgccgagt ttatgtaacg cctcctccag cgccccggcg accgttgatt taccggaccc
    19921 ggagaggccg gtaaaccaca gcactacacc acgatgaccg tggtgtagct cgcgttgttg
    19981 cacagtgacc ggatggctat gccagacgac gttttcgtca tgcagcgcca ttatttatcc
    20041 cccagcaaat cgcgcgcgcc ccagtgcgga aagtggcgac gaaccagagc attcaattcc
    20101 agttcgaatg cactgaattc agatggcgca gcagttgcct ggctaactgg ctcgtgcacc
    20161 ataccggcac ccacggtcac attgctcagg cgatcgataa aaatcagccc acccgtcacc
    20221 ggattttgtt gataacgatc taacaccagc ggctcgtcaa aagtgagatc cacgaggccg
    20281 atcccattca gtggcaggtt ttcaacttca cgctgggtaa ggttattaat atcaacctga
    20341 tagcgaatgc catcaacacg cgcgcgcgtc ttcttaccgg caattttgat gtcgtaactc
    20401 tgccctggag aaagcggctg ttccgccatc cataccacat ccaccgacgc gctctgcacc
    20461 gccggtaacg cttcgtctgc cgccagcagc agatcgccac ggctgatgtc gatctcatcc
    20521 gtcagcacca gggtgatcgc ttctccggca aaggcttctt cgcgatcacc atcaaaagtc
    20581 acgatccgcg cgacgtttga ttccacacca gagggcagca ctttgacacg ttgcccgact
    20641 tccacgcgac cggatgccag cgttccggcg taaccacgaa aatcgagatt cgggcgatta
    20701 acgtactgca ccgggaagcg cattggctga gcatccacca ctcgctggat ctccacggtt
    20761 tccagcactt cgagcagtgt cggaccgctg taccacggca tactttcact ttgcgatgcc
    20821 acgttgtcgc cttccagtgc agagagcggc acaaagcgga tatccagatt acccggcagc
    20881 tgcccggcaa aggtcaaata atcttcacga atacgggtga acgtctcttc actgtaatcc
    20941 accagatcca ttttgttgat cgccacgacc agatgtttgc tccccaacag tgtggagata
    21001 aaactgtgac gacgggtttg atcgagcacg cctttacggg catcgatcag taagatcgcc
    21061 agttcacatg tcgatgcgcc agtcgccata ttgcgggtgt actgctcgtg ccctggggtg
    21121 tcggcgataa taaatttacg cttctcggta gagaaatagc ggtaggccac gtcaatggtg
    21181 atgccctgtt cgcgctcagc ttgcaggccg tccaccagca gagccagatc cagcttttcg
    21241 ccctgggtgc cgtgacgctt actgtcgtta tgcagcgatg agagctgatc ttcgtagatt
    21301 tggcgggtat cgtgcagcag acgaccaatc agagtacttt tgccgtcatc gacgctacca
    21361 caggtcagaa aacgcagcag gcttttatgt tgttgcgcaa tcatccaggc ttcgacgccg
    21421 ccttcattgg cgatttgttg tgcaagtgcg gtgttcatct taaaaatacc cctgacgttt
    21481 tttcagctcc atagaccccg cctggtcgcg gtcaatcacg cggccctgac gttcactggt
    21541 ggtggaaacc agcatctctt cgatgatttc cggcagtgtt tgtgcatttg actccaccgc
    21601 accggtcagc ggccagcagc ccagcgtacg gaaacgcacc atccgttttt taatcacttc
    21661 gcccggttgc aggtcgatac ggttgtcatc aatcatcatc aacataccgt cgcgttccag
    21721 aaccggacgt tccgcagcga gatatagcgg aacaatgtcg atattttcca gccagatgta
    21781 ttgccagata tcctgctcgg tccagttaga gagcgggaag acgcggatgc tttcgccttt
    21841 gttaatttgc ccgttgtagt tgtgccacag ctccgggcgc tgatttttcg gatcccagcg
    21901 atggaagcgg tcacggaaag agtaaattcg ctctttagcg cgggatttct cttcgtcacg
    21961 gcgcgcacca ccgaaggcgg catcaaaacc gtatttgttc agcgcctgtt tcaggccttc
    22021 agttttcata atatcggtat gtttcgcgct gccgtgcacg aatggattaa tccccatcgc
    22081 cacgccttcc gggtttttat gcaccagcag ttcgcagccg taggctttag cagtacgatc
    22141 gcggaactca tacatctcgc ggaatttcca gccggtatcg acatgcagca acgggaaagg
    22201 cagcgtacct ggataaaacg ccttgcgcgc cagatgcagc atgacgctgg aatctttacc
    22261 gatagagtag agcatcaccg gatttgagaa ttctgccgcc acctcgcgaa taatgtggat
    22321 gctttccgcc tccagttgcc gcaggtgagt aagtcgtatt tgatccataa ccgttccttt
    22381 gcaataccgc tattttcttg ccatcagatg tttcgactat agggagcgta agagaacgaa
    22441 tgaaattacc aattagaatg agtagttcct taacggaata acgatttggc aaagctaata
    22501 tcaaaaagtg cttaaggcac cggatttcgg gcgtttagga agatttgaaa ttgttttagc
    22561 gcagcggcag tttcatacta tggcggtaaa aaaatttgca tggtatttaa ggactcacta
    22621 tgttttccgc attgcgccac cgtaccgctg ccctggcgct cggcgtatgc tttattctcc
    22681 ccgtacacgc ctcgtcacct aaacctggcg attttgctaa tactcaggca cgacatattg
    22741 ctactttctt tccgggacgc atgaccggaa ctcctgcaga aatgttatct gccgattata
    22801 ttcgccaaca gtttcagcaa atgggttatc gcagtgatat tcggacattt aatagtcggt
    22861 atatttatac cgcccgcgat aatcgcaaga gctggcataa cgtgacggga agtacggtga
    22921 ttgccgctca tgaaggcaaa gcgccgcagc agatcatcat tatggcgcat ctggatactt
    22981 acgccccgct gagcgatgct gacgccgatg ccaatctcgg cgggcttacg ttacaaggaa
    23041 tggatgataa cgccgcaggt ttaggtgtca tgctggaatt ggcagaacgc ctgaaaaata
    23101 cgcctaccga gtatggtatt cgatttgtgg cgaccagcgg cgaagaggaa gggaaattag
    23161 gcgcagagaa tttactcaag cggatgagtg acaccgaaaa gaaaaatacg ctgctggtga
    23221 ttaatctcga taacttaatt gttggcgata aattgtattt caacagcggt gtaaaaaccc
    23281 ctgaagcagt aaggaaatta acgcgcgaca gggcgctggc aattgcgcgt agtcatggaa
    23341 ttgccgcaac gaccaatccg ggtttgaata aaaattatcc gaaaggcact gggtgttgta
    23401 atgacgcaga aatattcgac aaagcgggca ttgctgtact ttcggtggaa gcgactaact
    23461 ggaatcttgg gaataaggat ggttatcagc aacgcgcaaa aacacctgcc ttcccggcgg
    23521 gaaatagctg gcatgacgta agactggata atcaacaaca tattgataag gcacttcctg
    23581 gaagaataga acgtcgctgc cgtgatgtta tgcggataat gctaccgctg gtgaaggagc
    23641 tggcgaaggc gtcttgatgg gtttgaaaat gggagctggg agttctaccg caggggcggg
    23701 gaattctaag tgatatccat catcgcatcc agtgcgcccg gtttatcccc gctgacgcgg
    23761 ggaacacaaa accaaacttc tccataaatt ccatagccgc ggtttatccc cgctggcgcg
    23821 gggaacacga gagtgctgac aggtgtctcg attacctgat cggtttatcc ccgctggcgc
    23881 ggggaacacg gtagtacgcg cctccggacg tttttatgtc gcggtttatc cccgctggcg
    23941 cggggaacac tcttcgcggg taatcaatga tgattcagtt tccggtttat ccccgctggc
    24001 gcggggaaca cgaacgcaat atcacggcgt tctgcggcct cggcggttta tccccgctgg
    24061 cgcggggaac accaaaatat tacgagcttc gtcaggccat ggaccggttt atccccgctg
    24121 gcgcggggaa cacgtcgggc cgggtaagtg aacctgtcgg ttttccggtt tatccccgct
    24181 ggcgcgggga acacactaag catacataac tgttt
//
LOCUS       DAZKGS010000063        23738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKGS010000063 DAZKGS010000000
VERSION     DAZKGS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(107..2176)
                     /gene="glyS"
                     /locus_tag="ACHOQY_002470"
     CDS             complement(107..2176)
                     /gene="glyS"
                     /locus_tag="ACHOQY_002470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9678551.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2186..3097)
                     /gene="glyQ"
                     /locus_tag="ACHOQY_002471"
     CDS             complement(2186..3097)
                     /gene="glyQ"
                     /locus_tag="ACHOQY_002471"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9678552.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3192..3491)
                     /locus_tag="ACHOQY_002472"
     CDS             complement(3192..3491)
                     /locus_tag="ACHOQY_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9678553.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3666..4661
                     /gene="wecH"
                     /locus_tag="ACHOQY_002473"
     CDS             3666..4661
                     /gene="wecH"
                     /locus_tag="ACHOQY_002473"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9678554.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLFLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4703..5140)
                     /gene="yiaA"
                     /locus_tag="ACHOQY_002474"
     CDS             complement(4703..5140)
                     /gene="yiaA"
                     /locus_tag="ACHOQY_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9678555.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5186..5527)
                     /gene="yiaB"
                     /locus_tag="ACHOQY_002475"
     CDS             complement(5186..5527)
                     /gene="yiaB"
                     /locus_tag="ACHOQY_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9678556.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5696..7150)
                     /gene="xylB"
                     /locus_tag="ACHOQY_002476"
     CDS             complement(5696..7150)
                     /gene="xylB"
                     /locus_tag="ACHOQY_002476"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9678557.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7222..8544)
                     /gene="xylA"
                     /locus_tag="ACHOQY_002477"
     CDS             complement(7222..8544)
                     /gene="xylA"
                     /locus_tag="ACHOQY_002477"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9678558.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8910..9902
                     /gene="xylF"
                     /locus_tag="ACHOQY_002478"
     CDS             8910..9902
                     /gene="xylF"
                     /locus_tag="ACHOQY_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9678559.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9980..11521
                     /gene="xylG"
                     /locus_tag="ACHOQY_002479"
     CDS             9980..11521
                     /gene="xylG"
                     /locus_tag="ACHOQY_002479"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9678560.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11499..12680
                     /gene="xylH"
                     /locus_tag="ACHOQY_002480"
     CDS             11499..12680
                     /gene="xylH"
                     /locus_tag="ACHOQY_002480"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9678561.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12758..13936
                     /gene="xylR"
                     /locus_tag="ACHOQY_002481"
     CDS             12758..13936
                     /gene="xylR"
                     /locus_tag="ACHOQY_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9678562.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14044..14868)
                     /locus_tag="ACHOQY_002482"
     CDS             complement(14044..14868)
                     /locus_tag="ACHOQY_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9678563.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15188..17218
                     /gene="malS"
                     /locus_tag="ACHOQY_002483"
     CDS             15188..17218
                     /gene="malS"
                     /locus_tag="ACHOQY_002483"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9678564.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17396..18649
                     /gene="avtA"
                     /locus_tag="ACHOQY_002484"
     CDS             17396..18649
                     /gene="avtA"
                     /locus_tag="ACHOQY_002484"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9678565.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18801..19274)
                     /locus_tag="ACHOQY_002485"
     CDS             complement(18801..19274)
                     /locus_tag="ACHOQY_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9678566.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19376..20224)
                     /gene="yiaJ"
                     /locus_tag="ACHOQY_002486"
     CDS             complement(19376..20224)
                     /gene="yiaJ"
                     /locus_tag="ACHOQY_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP9678567.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20425..21423
                     /gene="yiaK"
                     /locus_tag="ACHOQY_002487"
     CDS             20425..21423
                     /gene="yiaK"
                     /locus_tag="ACHOQY_002487"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP9678568.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21435..21902
                     /locus_tag="ACHOQY_002488"
     CDS             21435..21902
                     /locus_tag="ACHOQY_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP9678569.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22020..22493
                     /gene="yiaM"
                     /locus_tag="ACHOQY_002489"
     CDS             22020..22493
                     /gene="yiaM"
                     /locus_tag="ACHOQY_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP9678570.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22496..>23738
                     /gene="yiaN"
                     /locus_tag="ACHOQY_002490"
     CDS             22496..>23738
                     /gene="yiaN"
                     /locus_tag="ACHOQY_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP9678571.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIP"
BASE COUNT         5894 a         5999 c         5830 g         6015 t
ORIGIN      
        1 tatatgagat aatctttatt cagcagaaga ttattaaagg ttgctgtatt atttagcgat
       61 aataaaaaaa gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg
      121 aaatatccgc aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga
      181 gacgcaattc tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct
      241 cacgcagctc tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct
      301 ccagcttgtc acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca
      361 gggtagaggc attcacgcgg tcgctcagca cttcgtcaga tttcgccaaa atgttggaga
      421 cacgcttgtt cgccgccgcc agtgcagcag ctgcttcaag ggtacggaag tgcgataccg
      481 ctttcatgcg ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga
      541 tggtgtcaac ggtgtaacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt
      601 cgataacatc atcaaccacg ttggcgttag tcagcttatc gccatacaga cgcactgctt
      661 cttcggtcag cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc
      721 caagtgcggc acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tggccgatac
      781 cgaagatacc cgccagagta tccatcttgt cagcaatcgc cagcgcacag gctaccgggt
      841 tagacggcag gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gccaccgcga
      901 catcttccgc ttcgccatcg tgacgcgcat agtgcatccc cataacgccc tgggtgtcgg
      961 tgaactcgaa gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg
     1021 cgtggttaac gtcagcgcca atctgttcag caatccagcc agccagcgcc tggatgcggt
     1081 cagttttgtc gcgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca
     1141 ggttatcttc cagacgtttt ttacggtcgg tattgaagaa gaactcggca tccgccagac
     1201 gtggacgaac gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt
     1261 tggcaacgaa gatgaagttc ggcagcagtt tgccatcgtt cgcatacacc gggaagtatt
     1321 tctggtcacc tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt
     1381 cgaattttgc ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc
     1441 tttcgcttaa gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa
     1501 tcttcgcttt acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg
     1561 gatactgatc ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa
     1621 tcacgcgatc ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca
     1681 gggtcacggt gtgcaccgga cgcacgaagt gcacgtcgct tgcgccccaa cgcatcagtt
     1741 tcgggatcgg cagtttcgcc agagaagtcg caaccatatt cggcagcagt gcttcggtgc
     1801 tttcgccctt cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct
     1861 cagcctggtc aacggtaata ccgcaaccac gcgcccaacc ttctgccgct ttgctcggtt
     1921 tgccttcagc gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatccg
     1981 gttgcgcttc cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt
     2041 gaacggtgcc gtgtgcgagg ccagcgttat ccagctccgc agtaaaattc gcagcaaagg
     2101 actcagccag gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa
     2161 aagttttctc agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg
     2221 cttcacggga agcgtagtat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa
     2281 tgtagcgctg acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt
     2341 gggcggcttt cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca
     2401 gctgctgcgc ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt
     2461 attcgaagtt gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg
     2521 ttttacccag cgggccgtcg ctccagacca ggtcgtaaac gctgtctacg ccctgaatgt
     2581 acatggccag acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac
     2641 caccaacctg ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc
     2701 agcccagtcc ccaggcaccc agagtcgggt tttcccagtt atcttccacg aaacggatgt
     2761 cgtgaatagt cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat
     2821 tgtccggcga tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt
     2881 tttcgccgta gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg
     2941 gttctggccc cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca
     3001 tgtccaatgg ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga
     3061 tcaagccctg gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt
     3121 ggatttaaag gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga
     3181 aaaagcaggg cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag
     3241 acaaactgct ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg
     3301 aaggcggtga cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg
     3361 cataacgctt ccatactcaa cgaacgcaga ggatcaacct ttacccgttg tgctttctgc
     3421 acgggtgacg gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg
     3481 ttcatcatca ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata
     3541 gtggctgata tcttacgaat ttccttaagc ctctataagc tgtagtgata aaactcagat
     3601 tattccagtg cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga
     3661 cacagatgca gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg
     3721 tggtgatgat tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca
     3781 catgggatat cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca
     3841 tgatttccgg ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta
     3901 tcggcttatt tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct
     3961 ccatcaatat ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt
     4021 ggtttttctt cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg
     4081 tcggcggaaa aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca
     4141 cagtgccgca gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg
     4201 atacttttta ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac
     4261 agcataaagc actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct
     4321 ctcgcgggac attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt
     4381 actgtgggcc gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc
     4441 tggatacgcg taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg
     4501 gattccacgc cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc
     4561 caatactgga tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta
     4621 tgctggtaca acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt
     4681 ggactgaccg ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt
     4741 ttggcgggtt tattccggta tcacgaatat tcttctgcac cgctactgca ccaaacaagc
     4801 ttaagaagaa agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca
     4861 gacctaccat cagtaatgca acagagataa taaagacagt caggcaggtc atataataaa
     4921 tggaagtggt cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag
     4981 aaaacagtcc taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg
     5041 cattccatag ccctaacaga taggtaacga taccaccaac gagagctatc catgacacaa
     5101 tactaaaggc cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc
     5161 tgaatgtgta agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca
     5221 agcagacaaa aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta
     5281 aaacaccgat aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa
     5341 aaaaattatc cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg
     5401 ctgtcattaa cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata
     5461 gcccaaccag ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg
     5521 ttttcataac gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa
     5581 actgaaaaat gccattaaca aatgattttc agaataaatt catactaaat attaattaat
     5641 tagtgagata tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg
     5701 ccattaatgg cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag
     5761 cggcataacg ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact
     5821 caatgagcga tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg
     5881 gccccacatc cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct
     5941 gacgccagta ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc
     6001 cgcaggcatg cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg
     6061 ctcgcgccag ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct
     6121 ggggattatt gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg
     6181 cactttcatc agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta
     6241 atttcgcgac ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac
     6301 gctgcggtag cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt
     6361 cgctgacagc aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa
     6421 ccattcccac accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg
     6481 tcgccatacc ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt
     6541 cgtataatgc gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc
     6601 agtcacgctt tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa
     6661 actcccccgt catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac
     6721 ggaatatctc cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca
     6781 tcatcaggtt gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact
     6841 cttgcgcaca gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat
     6901 ccagtaaggt tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag
     6961 aatgctgatc gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt
     7021 cttgttccga ccagagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa
     7081 ccacctcacc ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc
     7141 cgatatacat atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc
     7201 aacggactgc acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag
     7261 ttgctcctgg cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc
     7321 taaatctgcc agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc
     7381 ggaataacgc tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat
     7441 tttcagcgcc actgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt
     7501 atcagtactt tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccacc
     7561 tgctttgaga atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc
     7621 ggtgtcccag cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag
     7681 cgcaatggcg gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc
     7741 ttcaatgttc agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt
     7801 cgcggcatcg taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa
     7861 cgtgccctgg aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag
     7921 ttgttcacgc tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc
     7981 ccacaggaca tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg
     8041 cgttgccgcc cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg
     8101 agggtttgta aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt
     8161 ggctgccagg acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc
     8221 ctcaggggaa acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa
     8281 ctcaaatgcg acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca
     8341 cggacgatta aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca
     8401 gtagcaggcg gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg
     8461 attgtagtga cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc
     8521 gagctggtca aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg
     8581 gatgatgtcg taatattggg cactcccttt cagttgctca attatgttat ttcacactgc
     8641 tattgagata attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaactgg
     8701 gtaattcctc gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg
     8761 tcataaatca agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt
     8821 cccctgttta gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc
     8881 tgtcctctaa ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt
     8941 gcacctcact tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg
     9001 cgattgatga tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg
     9061 cagaatctct cggcgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa
     9121 tgtcgcagat tgaaaacatg ataaaccggg gtgttgatgt tcttgtcatt attccgtata
     9181 acggtcaggt attaagtaac gttgtaaaag aagccaaaca agaaggtatt aaagtattag
     9241 cttacgatcg tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa
     9301 aagtcggtga actgcaggca aaagccctgg tcgatattgt tccgcaaggt aattacttcc
     9361 tgatgggcgg ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag
     9421 tgttaaaacc ttacgttgat tccggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg
     9481 gctggttacc ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca
     9541 aaattgatgc tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa
     9601 gcgcgcaagg tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta
     9661 ttaaacgtat tgctgccggt acgcaaacta tgacggtgta taaacctatt acgttgttgg
     9721 caaatactgc cgcagaaatt gccgttgagt tgggcaatgg tcaggaacca aaagcagata
     9781 ccacactgaa taatggcctg aaagatgtgc cctcccgcct cctgacaccg atcgatgtga
     9841 ataaaaacaa catcaaagac acggtagtta aagacggatt ccacaaagag agcgagctgt
     9901 aagcgttacg ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat
     9961 tggcttaggt ggagtcgtta tgccttatct acttgaaatg aagaatatta ccaaaacctt
    10021 cggcagtgtg aaggcgattg ataacgtctg cttgcggttg aatgctggcg aaatcgtctc
    10081 actttgtggg gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta
    10141 tccccatggc tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca
    10201 catccgcgat accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa
    10261 agaattgacc gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat
    10321 ggattatgac ctgatgacgc tacgctgtca gaagctgctc gcacaggtca gtttatccat
    10381 ttcacctgat acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc
    10441 caaggcactt aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac
    10501 tgagcaggaa acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc
    10561 ctgtatttat atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt
    10621 tattcgcgac ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat
    10681 tatcaccatg atggtcgggc gagagttaac cgcgctttac cctaatgaac cacataccac
    10741 cggagatgaa atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat
    10801 taaacgagtt aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg
    10861 actcgttggt gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca
    10921 atgggaagga aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc
    10981 catcgcccag gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt
    11041 aatggcggtt ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag
    11101 ccagcttgat gacgcggaag agcaaaaatg tattctggaa tcaattcagc aactcaaagt
    11161 taaaacgtcg tcccccgatc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc
    11221 gatcctcgct cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag
    11281 gggtatcgat attggcgcga aatacgagat ctacaaatta attaaccaac tcgtccagca
    11341 gggtattgcc gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg
    11401 ggtactggtg atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca
    11461 ggagcaggtg atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg
    11521 aagtgaaatt ggccgtaccg acatccggtg gcttttccgg gctgaaatca ctgaatttgc
    11581 aggtcttcgt gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg
    11641 atggtgccta cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg
    11701 gcatcctcgc ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg
    11761 gctcaatgat ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc
    11821 ctttgccact taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg
    11881 gatggtgggt cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg
    11941 catttcgcgg catactcatt ggcatcacca acggcacgac tgtttccccc accagcgccg
    12001 cgatgtcaca aattgggcaa agctatctac ctgccagcac tggcttcatc attggcgcgc
    12061 ttggcttaat ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg
    12121 gtttgcagtc tccggcctct accgcagtag tcggtcgcca ggctttaacc gctatcatcg
    12181 tattaggcgc aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc
    12241 tgacattgct gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca
    12301 tttatgccat cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca
    12361 ccaaacttgc cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta
    12421 gttctcgact tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg
    12481 cagcatgcgt gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag
    12541 taatgggggc atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga
    12601 ccttctggca gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg
    12661 caaccaaacg ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg
    12721 gttttgagtt tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat
    12781 cacattactg ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga
    12841 atatttacag gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg
    12901 cattgataaa atcaaggact ggttaggaga tggcgtcatt gccgacttcg acgacaaaca
    12961 gatcgagcaa gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca
    13021 ccttgcagaa agttacccac ccgttcatta cattgccacc gataactatg cgctggttga
    13081 aagcgcattt ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc
    13141 ggaatcaagc ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc
    13201 cgaagaaaag tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca
    13261 acatgcgcaa aatcggctgg cagactggct acaaacgctg ccaccgcaaa ccgggattat
    13321 tgccgttact gacgcccggg cgcggcatat tctgcaagta tgtgaacatc tacatattcc
    13381 cgtaccggaa aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc
    13441 gcgtgtcgcc ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa
    13501 actgttgcat cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc
    13561 agttcgcgtc attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat
    13621 tcaggccatg cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact
    13681 ggatgcggtc gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga
    13741 aaccatccat gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc
    13801 aaccaccttg tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt
    13861 ctactctgtt tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag
    13921 cgaggtcatg ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag
    13981 ccacctgatg cgagcaaaag ccttatccga ccgacaaatc agataaggct caaggaaatg
    14041 caattacata tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt
    14101 gttataactc ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt
    14161 cacttcctga tccgctttac gcagctgcgc acgcgattta cggaatgaag agtaagccgg
    14221 atgcgtattg aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga
    14281 gtaccctttc actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa
    14341 cagattgttg ttgttgcgcg ccagctttga cgttccccaa ccgctttctg ctgcagccat
    14401 cgtcgccacc atactggtgg ggataatgtc tacgcgttca agcaaggtat tccacgggat
    14461 ttttcgcgta ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc
    14521 acgctcagca ggtgaccatt ggccctgata ctgttttgaa atcagccagt tacgttccgc
    14581 agtaatggct gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt
    14641 ccttggtgtt ccggaagggt attttcgcaa atcaggaagt gaattactct ttgcactatt
    14701 gcgagaatac tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt
    14761 ttgactcgct gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag
    14821 taacataaga atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg
    14881 tcggatttaa tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat
    14941 catatcaaaa atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca
    15001 cgagttaacg attcagattg gcgctaaatc gcagaaaatg tggggtttat cgcaaaattc
    15061 agccgttttt tgcgcgagat cgctcaccct tgcttctcat cctgtggact taccgctcag
    15121 ggatgagttt tgtttggctt atcgctggca aactgtctga aatcgcagca ataaggactc
    15181 atccgccatg aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc
    15241 cagctggact tctccggggt tccctgcctt tagtgaacag ggaacggaaa catttgtcag
    15301 ccacgcgcag ttgcccaaag gtacgcgtcc actaacgcta aattttgacc aacagtgctg
    15361 gcagcctgcg gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac
    15421 gcagcctcaa tggcgattgt tcagggacgg cgaatatacg ctgcaaatag acacccgctc
    15481 cggcacgcca acattgatga tttccatcca gaacgccgcc gaaccggtag caagccaggt
    15541 ccgtgtatgc ccgaaatggg atggattacc gctcacgctg gatgtcagcg ccactttccc
    15601 ggaaggagcc gccgtacggg attattacag ccagcaaatt gcgatagtga agaacggtca
    15661 aataacgtta caacccgctg ccaccagcaa cggtttactc ctgctggaac gggcagaaac
    15721 tgacgcctct gcccctttcg actggcataa cgccacggtt tactttgtgc tgacagatcg
    15781 tttcgaaaac ggcgatccca gtaatgacca gagttacgga cgtcataaag acggtatggc
    15841 ggaaattggc acttttcacg gcggcgattt acgcggcctg accaacaaac tggattacct
    15901 ccagcagttg ggcgttaatg ctttatggat aagcgcccca tttgagcaaa ttcacggctg
    15961 ggtcggcggc ggtacaaaag gcgatttccc gcattatgcc taccacggtt attacacaca
    16021 ggactggacg aatcttgatg ccaatatggg caacgaagcc gatctacgga cgctggttga
    16081 tagcgcacat cagcgcggta ttcgtattct ctttgatgtc gtgatgaacc acaccggcta
    16141 tgccacgctg gcggatatgc aggagtatca gtttggcgcg ttatatcttt ctggtgacga
    16201 ggtgaaaaaa acgctgggtg aacgctggag cgactggaaa cctgccgccg ggcaaacctg
    16261 gcatagcttt aacgattaca ttaatttcag cgacaaaaca ggctgggata aatggtgggg
    16321 aaaaaactgg atccgtaccg atatcggcga ttacgacaat cctggattcg acgatctcac
    16381 catgtcgcta gcctttttgc cggatatcaa aaccgaatca actaccgctt ctggtctgcc
    16441 ggtgttctat aaaaacaaaa cggataccca cgctaaagcc atcgacggct ttacccctcg
    16501 cgattactta acccactggt taagtcagtg ggtccgcgac tatgggattg atggttttcg
    16561 ggtcgatacc gccaaacatg ttgagttgcc cgcctggcag caactgaaaa ccgaagccag
    16621 cgccgcgctt cgcgaatgga aaaaagctaa ccccgacaaa gcattagatg acaaaccttt
    16681 ctggatgacc ggtgaagcct ggggccacgg cgtgatgcaa agtgactact atcgccacgg
    16741 cttcgatgcg atgatcaatt tcgattatca ggagcaggcg gcgaaagctg tcgattgtat
    16801 tgcgcagatg gatatgacct ggcagcaaat ggcggagaaa ttgcagggtt tcaacgtgtt
    16861 gagctacctc tcgtcgcatg atacccgtct gttccgtgaa gggggcgaca aagcagcaga
    16921 gttattacta ttagcgccag gcgcggtaca aatcttttat ggcgatgaat cctcgcgtcc
    16981 gttcggtcct acaggttctg atccgctgca aggtacacgt tcggatatga actggcagga
    17041 tgttagcggt aaatctgccg ccaacgtcgc gcactggcag aaaatcagcc agttccgcgc
    17101 ccgccatccc gcaattggcg cgggcaaaca aacgacactt tcgctgaagc agggctacgg
    17161 ctttgttcgt gagcatggcg acgataaagt gctggtcatc tgggctgggc aacagtgact
    17221 tttccggctt cccgttcgtc agtacctcgg gaagccgcca accaggataa aatgtcagcc
    17281 ctaatcagcg ttgcaggata aagcaccgct cactcttcaa cagaccgatt tgcaccccag
    17341 caaatgtagc gttattgtta ccttccttgc tacagagttc gacagatatc ccgctatgac
    17401 attctccctt tttggtgaca aatttacccg ccactccggc attacgctgc tgatggaaga
    17461 tctgaacgac ggtttacgca cccctggcgc gattatgctc ggcggcggta atccggcgca
    17521 gatcccggaa atgcaggact acttccagac gctactgacc gacatgctgg aaagtggcaa
    17581 agcgactgat gcactgtgca attacgacgg tccacagggg aaaacggagc tactcacact
    17641 gcttgccgga atgctgcgcg agaagttggg ttgggatatc gaaccacaga atattgcact
    17701 aacaaacggc agccagagcg cgtttttcta cttatttaat ctgtttgccg gacgccgtgc
    17761 cgatggtcgg gtcaaaaaag tgctgttccc gctggcaccg gaatacattg gctacgctga
    17821 cgccggactg gaagaagatc tgtttgtctc tgcgcgtccg aatattgaac tgctgccgga
    17881 aggccagttt aaataccacg tcgattttga gcatctgcat attggcgaag aaaccgggat
    17941 gatttgcgtc tcccggccga cgaatccaac aggcaatgtg attactgacg aagagttgct
    18001 gaagcttgac gcgctggcga atcaacacgg cattccgctg gtgattgata acgcttatgg
    18061 cgtcccgttc ccgggtatca tcttcagtga agcgcgcccg ctatggaatc cgaatatcgt
    18121 gctgtgcatg agtctttcca agctggggct gcctggctcc cgctgcggca ttatcatcgc
    18181 caatgaaaaa atcatcaccg ccatcaccaa tatgaacggc attatcagcc tggcacctgg
    18241 cggtattggt ccggcgatga tgtgtgaaat gattaagcgt aacgatctgc tgcgcctgtc
    18301 tgaaacagtc atcaaaccgt tttactacca gcgtgttcag gaaactatcg ccatcattcg
    18361 ccgctattta ccggaaaatc gctgcctgat tcataaaccg gaaggagcca ttttcctctg
    18421 gctatggttt aaggatttgc ccattacgac cgagcagctc tatcagcgcc tgaaagcacg
    18481 cggcgtgctg atggtgccgg ggcacaactt cttcccaggg ctggataaac cgtggccgca
    18541 tacgcatcaa tgtatgcgca tgaactacgt accagagccg gagaaaattg aggcgggggt
    18601 gaagattctg gcggaagaga tagaaagagc ctgggctgaa agtcactaaa aattgcctga
    18661 tgcgctacgc ttatcaggcc tacatgattt ctgcaatata ttgaatttgg aagaatttgt
    18721 aggccggata aggcgtttac gccgcatctg gcataaacaa agcgcactat tccggcgttg
    18781 agaaacgccg gaaaaacgtt tcaaacaggc tgctgccgta gccgatgccg ctgcactttc
    18841 tcgacatcca tgcactgcaa cgcctttgtc gggcaggctt caacacaggc cggtcccgtc
    18901 tcccgatgcc agcacagatc gcacttaatc gccctcgcct ttttgcgcga agaaacgacc
    18961 tccatcgcac caaacgggca agccagcata cagcttttac agccaatgca acgtgtttgt
    19021 tcaacgaaaa tatgcccatg ttcgcggctt atcgcgtcaa cagggcagac attcgcgcac
    19081 ggtgcatctt cacactgatg acaggctact gccgtggtcc agctgtggtc tttaatgaca
    19141 cgaatacggg aaataaactc gtctggtgac aacgcagcgc aatcctgatt ctcctgatgc
    19201 gacactgcgc aagccacttc acaggtacgg caaccgatac atttcgtcgc atccgcaata
    19261 ataaaccggt tcatgatcac tcctttgcac taaaacagca gagtgccaga gagcgcgtcc
    19321 ctactgcttt gatccaacga aggaaatgtt aaagttttgg cacttgtcca aagcgttatg
    19381 tgatcgcgcc caggtcatcc ctgacagtaa atcccagttc attagaaata gcctgcgcgg
    19441 tttcacgcag tggtttcagg agatttttct ctcccacctg tttcagacgt gatgtcgaaa
    19501 gcgaaatcga cacggcgtac ggcacccgcc catgaatatc aaacaccgga acagcaatac
    19561 aggagacgcc gagttcgttt tcttctctgt ccatcgccgc tccgctttca cgaatgtgcg
    19621 ccagttcgtc gaacatcgcg ggcagctcgg taatggtatt gcgggttaac ggctggatct
    19681 catgctgatg gttttcccag tatgacttca cgtagtccgg gtgaccaaac gccatgtaga
    19741 tcttgcccat tgcggaacag tagagcggca tatgctggcc aatataggca cgggttcgca
    19801 gcatcccggt tgtgggttcc agcttataaa tcaaaatagc gtgatcgtct tcgcggctgg
    19861 agaagttaat ggtttcacca gtggcgatgt tcagtgcctc aagatgcgga gcggcgatat
    19921 gaatgatatt cagcgaagac agcgccttct gcccgacggc aataaatttg gtagtcaggc
    19981 ggtaactccc tgcggcgggc gcggtggtca catagccgca ggactgtaat ccctgcaata
    20041 agcgatggac ggtactctta tttaaaccag ccagctccga aagatgcgcc aacggacaac
    20101 cgtttggata gttgctcaaa atctcaatca gcatcaaccc gcgaaacaga ctctggcttc
    20161 cggctggacg ctctttttcc tgcgccatct cgttctcttt ttttcccatc acttctttcc
    20221 ccattttgtc gcgtcctgat ggtagcgcaa agtgtgccgt agttcacgat ctcgacagat
    20281 aatttataac cacttgattt ttatgtcttt tgaaattcat caatcagatt gctttgttaa
    20341 aaagtgatcg atatatttga aatcaagttt cgcatattga aattttaagc caaaaaagcg
    20401 atcaaaaaaa caaggaagcc tgggatgaaa gtgacatttg agcagttaaa agcagccttt
    20461 aatcgggtct taatttcacg cggcgttgac agcgaaacgg ctgacgcctg tgcagagatg
    20521 ttcgcccgca ccaccgaatc cggcgtttat tctcacggcg ttaatcgttt ccctcgtttc
    20581 attcaacaac tggaaaacgg cgatatcatt cctgatgccc aacccaaacg tataaccagc
    20641 ctcggcgcaa ttgaacagtg ggatgcccag cgttcgatcg gcaacctgac ggcgaaaaag
    20701 atgatggatc gcgccattga actggctgcc gatcacggta ttggtctggt ggcacttcgt
    20761 aatgccaacc actggatgcg cggcggcagc tacggctggc aggcggcgga aaaaggctat
    20821 attggcattt gctggaccaa ctccatcgcc gtaatgccgc cgtggggcgc aaaagagtgt
    20881 cgcattggca ctaacccgct gatcgtcgcc attccttcca cgccgatcac catggtcgat
    20941 atgtcgatgt cgatgttctc ttacggcatg ttagaagtta accgcctggc gggccgtcag
    21001 ctcccggtcg atggtggctt tgatgatgag ggcaatttga ccaaagaacc tggcgttatc
    21061 gagaagaatc gccgcatttt gccgatgggc tactggaaag gttctggcat gtcgattgtg
    21121 ctggatatga tcgctactct cctttccgac ggcgcatcgg ttgccgaagt cacccaggac
    21181 aacagcgacg aatacggcat ttcacaaatt tttattgcca ttgaagtgga caagcttatc
    21241 gacggtccca cccgcgatgc caagctgcaa cgtatcatgg attacgttac taccgctgaa
    21301 cgcgctgacg aaaaccaggc catccgctta cccggccacg aatttactac cctgctggcc
    21361 gaaaaccgcc gtaacggtat taccgttgat gacagcgtgt gggccaaaat ccaggcgtta
    21421 taaggagata agtcatgatt tttggacata tcgcgcagcc caatccgtgc cgtttgcccg
    21481 ccgccattga aaaggcgctc gattttctgc gcgctaccga tttcaacgcc ctggagccgg
    21541 gcgttgtcga aatcgacggc aaaaatattt atgcgcaaat tatcgattta accacacgcg
    21601 aagcggtgga aaaccgtccg gaagtgcatc gtcggtatat cgatattcag tttctggcgt
    21661 ggggtgaaga gaaaatcggc attgctattg atacgggaaa taataaagtc agcgaatcac
    21721 tattagagca gcgcgatatt attttttatc atgacagtga acatgaatca tttatagaaa
    21781 tgataccggg cagctacgca attttctttc cgcaggatgt tcaccgacca ggttgtattt
    21841 tgcaaacagc ctctgagata cgaaaaattg tggttaaagt cgcattaacg gcgctgaatt
    21901 aatcgaataa tcgtctacgc cagaacgact gggtaatgtt attgctctaa ctatattaat
    21961 aatttaggtt gctgcacatg caacttgaat tatgacagac atagcctcaa ggaatagcta
    22021 tgaaaaaaat acttgaagca atactggcga ttaatctcgc cgtactttcc tgtattgtgt
    22081 ttataaacat tattttaaga tatggttttc agacaagcat tttatctgta gatgaattgt
    22141 cacgttattt atttgtctgg ttaacattta ttggcgcgat tgtggctttt atggataacg
    22201 cccacgttca ggtcaccttt ctggtggaga aactctcccc cgcatggcaa cgacgagtcg
    22261 ctctggttac ccattcctta atcttattta tttgtggcgc actggcctgg ggcgcaacgc
    22321 taaaaacaat tcaggactgg agcgattatt caccgatcct cggtttaccc atcggcctga
    22381 tgtatgccgc ttgtctgccc accagcctcg ttatcgcatt ctttgaactg cgtcatttat
    22441 atcaactcat cacgcgtagc aattctctaa cgtcgccacc gcaaggagct taatcatggc
    22501 tgtgctgatt tttctgggct gtctgttggg tggtattgct atcggtttgc ctattgcctg
    22561 ggcactgttg ttgtgcgggg cggcgttaat gttctggctg gacatgtttg atgtccagat
    22621 catggcacaa acgctggtga acggagccga tagcttctcc ctgctggcga ttccgttctt
    22681 tgtgctggcg ggtgaaatca tgaatgcggg cggcttgtca aaacgcattg ttgacctgcc
    22741 gatgaaactg gtggggcata aaccaggcgg gctgggctac gtcggcgtgc tggcagctat
    22801 gattatggcg agcctttctg gttctgccgt tgcggatacc gccgccgttg ccgctctact
    22861 ggtgccgatg atgcgcagcg ccaactaccc ggtcaaccgg gcggcggggc tgattgcctc
    22921 tggcggcatt atcgcgccaa ttattccccc ttccattccg tttattatct tcggcgtttc
    22981 cagcggatta tccatcagta agctgtttat ggcgggcatt gcccctggca tgatgatggg
    23041 cgcaacgctg atgcttacct ggtggtggca ggccagtcgc cttaatttac cccgccagca
    23101 aaaagcaacg atgcaggaaa tctggcactc ttttgtctcc ggtatctggg cgctgtttct
    23161 tccagtcatc attattggcg ggtttcgctc cggtctgttt acccctaccg aagccggggc
    23221 cgttgctgct ttctatgcgc tgtttgtcgc cacagttatt taccgtgaaa tgaccttcgc
    23281 cacactttgg catgtactca ttggcgcggc gaaaaccacc tcagtggtta tgtttctggt
    23341 ggcctcggca caagtttccg cctggctgat taccattgct gaactgccga tgatggtttc
    23401 tgatttactg caaccgttgg tcgattcacc gcgtctgctg tttatcgtca ttatggtggc
    23461 tatcctgatt gtcggcatgg tcatggattt aacgccgacg gtattaattc ttaccccggt
    23521 attaatgcct ttagttaaag aggcaggaat cgatccgatt tatttcggtg tcatgtttat
    23581 cattaactgc tcaatcggct taattacacc gcctatcggt aatgtactta acgttatttc
    23641 aggggtggca aaactcaaat tcgatgatgc agtcagaggc gttttccctt acgtcctggt
    23701 tttatattca ttattagtcg tatttgtttt tattcccg
//
LOCUS       DAZKGS010000064        22815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKGS010000064 DAZKGS010000000
VERSION     DAZKGS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            25..1230
                     /gene="yedE"
                     /locus_tag="ACHOQY_002491"
     CDS             25..1230
                     /gene="yedE"
                     /locus_tag="ACHOQY_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9678572.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1227..1460
                     /gene="yedF"
                     /locus_tag="ACHOQY_002492"
     CDS             1227..1460
                     /gene="yedF"
                     /locus_tag="ACHOQY_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9678573.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1567..2235
                     /gene="yedK"
                     /locus_tag="ACHOQY_002493"
     CDS             1567..2235
                     /gene="yedK"
                     /locus_tag="ACHOQY_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP9678574.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(2630..2944)
                     /gene="fliE"
                     /locus_tag="ACHOQY_002494"
     CDS             complement(2630..2944)
                     /gene="fliE"
                     /locus_tag="ACHOQY_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9678575.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3159..4817
                     /gene="fliF"
                     /locus_tag="ACHOQY_002495"
     CDS             3159..4817
                     /gene="fliF"
                     /locus_tag="ACHOQY_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP9678576.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4810..5805
                     /gene="fliG"
                     /locus_tag="ACHOQY_002496"
     CDS             4810..5805
                     /gene="fliG"
                     /locus_tag="ACHOQY_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9678577.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5798..6484
                     /gene="fliH"
                     /locus_tag="ACHOQY_002497"
     CDS             5798..6484
                     /gene="fliH"
                     /locus_tag="ACHOQY_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9678578.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6484..7857
                     /gene="fliI"
                     /locus_tag="ACHOQY_002498"
     CDS             6484..7857
                     /gene="fliI"
                     /locus_tag="ACHOQY_002498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9678579.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7876..8319
                     /gene="fliJ"
                     /locus_tag="ACHOQY_002499"
     CDS             7876..8319
                     /gene="fliJ"
                     /locus_tag="ACHOQY_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9678580.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8316..9443
                     /gene="fliK"
                     /locus_tag="ACHOQY_002500"
     CDS             8316..9443
                     /gene="fliK"
                     /locus_tag="ACHOQY_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9678581.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            9548..10012
                     /gene="fliL"
                     /locus_tag="ACHOQY_002501"
     CDS             9548..10012
                     /gene="fliL"
                     /locus_tag="ACHOQY_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9678582.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10017..11021
                     /gene="fliM"
                     /locus_tag="ACHOQY_002502"
     CDS             10017..11021
                     /gene="fliM"
                     /locus_tag="ACHOQY_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9678583.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11018..11431
                     /gene="fliN"
                     /locus_tag="ACHOQY_002503"
     CDS             11018..11431
                     /gene="fliN"
                     /locus_tag="ACHOQY_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9678584.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11434..11799
                     /gene="fliO"
                     /locus_tag="ACHOQY_002504"
     CDS             11434..11799
                     /gene="fliO"
                     /locus_tag="ACHOQY_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9678585.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            11799..12536
                     /gene="fliP"
                     /locus_tag="ACHOQY_002505"
     CDS             11799..12536
                     /gene="fliP"
                     /locus_tag="ACHOQY_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9678586.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12546..12815
                     /gene="fliQ"
                     /locus_tag="ACHOQY_002506"
     CDS             12546..12815
                     /gene="fliQ"
                     /locus_tag="ACHOQY_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9678587.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12824..13609
                     /gene="fliR"
                     /locus_tag="ACHOQY_002507"
     CDS             12824..13609
                     /gene="fliR"
                     /locus_tag="ACHOQY_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9678588.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13899..14522
                     /gene="rcsA"
                     /locus_tag="ACHOQY_002508"
     CDS             13899..14522
                     /gene="rcsA"
                     /locus_tag="ACHOQY_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9678589.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14566..14754)
                     /gene="dsrB"
                     /locus_tag="ACHOQY_002509"
     CDS             complement(14566..14754)
                     /gene="dsrB"
                     /locus_tag="ACHOQY_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9678590.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14917..15144
                     /gene="yodD"
                     /locus_tag="ACHOQY_002510"
     CDS             14917..15144
                     /gene="yodD"
                     /locus_tag="ACHOQY_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9678591.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15442..16257
                     /gene="yedP"
                     /locus_tag="ACHOQY_002511"
     CDS             15442..16257
                     /gene="yedP"
                     /locus_tag="ACHOQY_002511"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9678592.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(16254..17948)
                     /gene="dgcQ"
                     /locus_tag="ACHOQY_002512"
     CDS             complement(16254..17948)
                     /gene="dgcQ"
                     /locus_tag="ACHOQY_002512"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9678593.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(18186..18368)
                     /gene="yodC"
                     /locus_tag="ACHOQY_002513"
     CDS             complement(18186..18368)
                     /gene="yodC"
                     /locus_tag="ACHOQY_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9678594.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18447..19364)
                     /gene="yedI"
                     /locus_tag="ACHOQY_002514"
     CDS             complement(18447..19364)
                     /gene="yedI"
                     /locus_tag="ACHOQY_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9678595.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            19537..20457
                     /gene="yedA"
                     /locus_tag="ACHOQY_002515"
     CDS             19537..20457
                     /gene="yedA"
                     /locus_tag="ACHOQY_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9678596.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(20446..20916)
                     /gene="vsr"
                     /locus_tag="ACHOQY_002516"
     CDS             complement(20446..20916)
                     /gene="vsr"
                     /locus_tag="ACHOQY_002516"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9678597.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20897..22315)
                     /gene="dcm"
                     /locus_tag="ACHOQY_002517"
     CDS             complement(20897..22315)
                     /gene="dcm"
                     /locus_tag="ACHOQY_002517"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9678598.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22382..>22815)
                     /locus_tag="ACHOQY_002518"
     CDS             complement(22382..>22815)
                     /locus_tag="ACHOQY_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFP9678599.1"
                     /translation="FVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEAL
                     GAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTARG
                     KQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT         5428 a         6023 c         5951 g         5413 t
ORIGIN      
        1 cttctgagtt cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt
       61 aaattctggg cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt
      121 ggcattactg gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggccagctc
      181 ctgcagctgt ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa
      241 ggatcgccat taacccgcat cgacgggatg atgatcctcg gcatgtttgg cggctgcttt
      301 gccgcagcgc tgtgggccaa caatgtcaaa ctgcgcatgc cacgcagccg tatccgcatt
      361 atgcaggcca ttattggcgg tattatcgct ggttttggcg cgcgtctggc aatgggctgt
      421 aacctggcgg cgttctttac cggtattcct cagttctcgc tgcatgcctg gttctttgcc
      481 atcgccactg ccattggttc atggtttggc gcacgcttta ccctgctgcc catcttccgt
      541 attcccgtga aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag
      601 gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg gtatgctggg ctgggcgctg
      661 ctcacggcga tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt cggctttggt
      721 ttactgattg aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc
      781 ggacgtaccc atatggcgaa agcaatcatt atcggtatgg cggtgagtgc catcgggatc
      841 ttcagttacg tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta
      901 attggtggtt tactgtttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg
      961 atgtaccgcg cggtagaagg ccaggtgcac tactggtggg tcggtctggg caatgtgatc
     1021 ggctcgacga ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg
     1081 gacaaaatca acctgctgaa aacctttggc ccgatgggtg gcctgctggt gacatatttg
     1141 ctgttgttta ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg
     1201 gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata
     1261 tggtgggtga accctgccct tatccggcag tcgcaactct tgaggcgatg ccgcagttaa
     1321 aaaaagggga aatcctggaa gtggtgagcg actgtccaca gtcgatcaat aatattccac
     1381 tggatgcgcg taatcacggc tatacggtgc tggatattca gcaagacggg ccgaccattc
     1441 gttatttaat tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aacatcgttg
     1501 cctctgacac atagatttta cccgccccct ctcctattct tttcagtatg atctcaggag
     1561 gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct tgcgcttctc
     1621 gcggaagata ttgaacgcga tattccctac gatcccgaac ccattggcag atacaacgtc
     1681 gcgccgggaa ccaaagttct tctgctcagt gaacgtgatg aacaccttca tctggatccg
     1741 gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta
     1801 gaaactgcgg ccaccagtcg tatgtttaaa ccgctctggc aacatggtcg ggcaatctgt
     1861 tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaaacagcc ttattttatc
     1921 tatcgcgctg atggacaacc tgtttttatg gccgcgatag gtagcacacc gtttgagcgc
     1981 ggtgacgaag ccgaaggatt tttaattgtc actgcagcag cagaccaggg tctggtagat
     2041 attcatgacc gccgcccgct ggttctgtcg ccagaagctg cacaggaatg gatgcggcaa
     2101 gagattggcg gtaaagaagc atcagaaatt gctaccaacg gctgtgtgcc agcaaaccag
     2161 ttcacctggc acccggtatc gcgcgcggtg ggtaatatca aaaaccaggg agcggagtta
     2221 attcaaccgg tttgataagc caggagacta ttgatcaata atactgcgtc ataagaaatc
     2281 tctattagac aatagtagac gctccttcca catgcgccac aagaaacctc tattccagtg
     2341 acacaattac gcctgattat ttccctagcc cggaatatga ataaaaacat caacaaagat
     2401 taaattattc aaacgttact gttgcgtctg cggtcgtagc aacatccgac gtggtgatga
     2461 gtgttaaaag agacactctg atgttcagag gggttatcgc gtttacggat cgtggatggc
     2521 agaaaacttt caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc
     2581 catgccccaa gcggtgagat tggaagtaat taacaacagg ttacaaaacc tacacctgca
     2641 tggacatcac ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca
     2701 tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
     2761 cgagggtgaa tttttctgcc tgcgtgcggg cagctgtttg tgtatcgcta atgcgatcga
     2821 gcgtggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgaa tcctgcgcac
     2881 gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
     2941 acattttcgt ctcctggata atttctggta gcaaagccta ccagtaagtc aataagataa
     3001 aggcgctaaa taacaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
     3061 aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
     3121 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
     3181 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
     3241 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     3301 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     3361 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gttccggcag
     3421 ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa ggcggcgcgg
     3481 tcggtttcga actactcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
     3541 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
     3601 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
     3661 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
     3721 ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca
     3781 cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg cgcgatctta
     3841 atgatgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag
     3901 caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggttacg gcgcagctgg
     3961 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg
     4021 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
     4081 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
     4141 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc
     4201 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
     4261 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
     4321 tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt gaagatctga
     4381 cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc
     4441 cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag caagcgttta
     4501 tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt
     4561 ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa gctgtacagc
     4621 aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
     4681 aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg agccagcgta
     4741 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
     4801 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt
     4861 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg
     4921 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg
     4981 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
     5041 cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt
     5101 ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagt
     5161 gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg
     5221 aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
     5281 atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa
     5341 gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga
     5401 acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc
     5461 gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt cctgttcgaa
     5521 aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa
     5581 tcgctgttga tcgcactgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
     5641 atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt
     5701 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa
     5761 actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
     5821 aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca tggtcgagcc
     5881 ggaagaaacc atcattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca
     5941 aatgcaggcc catgagcaag gttatcaggc gggtattgcc gaaggtcgcc agcaaggtca
     6001 taagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg cagaggcgaa
     6061 gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
     6121 ccttgatgca cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcggc
     6181 acgtcaggtc atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca
     6241 acagttgttg cagcaagaac cgttattcag cggcaaaccg caactacgcg tgcaccccga
     6301 tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg gctggcgctt
     6361 gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct
     6421 cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt
     6481 gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg
     6541 gcgcagttgc ctgcggtacg tcgctacggg cgattaactc gcgctaccgg gctggtgctg
     6601 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgagcg ccagaacggc
     6661 agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
     6721 atgccgctgg aggaagtcga aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt
     6781 tcggcagaag ggctgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgt
     6841 gttctggacg gcagcggtaa accgctcgat ggcctcccct cccccgatac gacggaaacc
     6901 ggtgcgctga ttaccccacc atttaacccg ttgcaacgta caccgattga acatgtgctg
     6961 gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca gcgtatgggg
     7021 ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc acgttacacc
     7081 cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgcg gacgcgaagt aaaagatttt
     7141 attgagaata tcctcggtgc cgaagggcgt gcacgctccg tggtgattgc cgctccggcg
     7201 gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgaat tgccgaagat
     7261 tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg
     7321 gcccagcgtg aaattgcgct ggcgattggc gaaccacctg ccactaaagg ttatccaccg
     7381 tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggaat tagcggcggc
     7441 ggctcgatta ccgcgtttta taccgtgctc accgaaggcg atgaccagca ggaccccatt
     7501 gccgactctg cgcgggcaat cctcgacggt cacattgtgc tgtctcgccg actggcggaa
     7561 gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg
     7621 atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag
     7681 cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgac
     7741 aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat ttttgaacgc
     7801 gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc
     7861 aggagataac ggcagatggc agaacatggc gcgctggcga ccctgaaaga tctggcagaa
     7921 aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg tcagcaggcg
     7981 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaacagc
     8041 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
     8101 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
     8161 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
     8221 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
     8281 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttaatta
     8341 ccgccgacgt tgataacgcc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
     8401 ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac taccgacaaa gcggcccccc
     8461 agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctggtc agcgagattc
     8521 ttgccgatgc acaacaagcg gatttactga tccctgtgga tgaaacaccg cctgtcatca
     8581 acgacgaaca atccacatca acaccattaa ccaccgctca aacgatgacg ttggctgcgg
     8641 tggctgacaa caatacggca aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg
     8701 caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
     8761 atgtgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg
     8821 cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag ccattaacac
     8881 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca ccggtgaccg
     8941 ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgtgc
     9001 ctgtgttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
     9061 cgctgttcac ccgccagagg caacaaagtg cagagttgcg tctgcacccg caggatttag
     9121 gtgaagtgca aatctccctc aaagtggatg ataaccaggc acaaatccag atggtttcac
     9181 cgcatcagca tgtacgcgcc gcgctggaag cagcgcttcc ggtactgcgt acgcagctgg
     9241 ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc
     9301 agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa cctctggcgg
     9361 gggaagacga cgatacgctt ctggttcccg tctctataca agggcgcgta acaggcaaca
     9421 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
     9481 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
     9541 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
     9601 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
     9661 tcaggttgcc gctgacgaca aagcgcagca acgcgtcgta ccctccccgg tcttctacgc
     9721 gctggatacc ttcacggtca atctgggcga tgcggatcgc gtgctttata tcggcataac
     9781 cctgcgcctg aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg
     9841 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
     9901 caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg ttgccgggca
     9961 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
    10021 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
    10081 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
    10141 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
    10201 cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat ataaccgtcg
    10261 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
    10321 tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt tcaccgagtc
    10381 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
    10441 aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc
    10501 ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt
    10561 cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
    10621 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgc ctgcctttca
    10681 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
    10741 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg
    10801 tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggtg
    10861 acgtcctgcc gatagaaaaa cccgaccgca tcatcgccca tgttgacggc gtcccggtgc
    10921 tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa catttgatta
    10981 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    11041 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gctttgagcg aacaaaaatc
    11101 aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    11161 cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgagct
    11221 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt ccgtcgtggc
    11281 gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt taattgccca
    11341 gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata tcattactcc
    11401 gtctgagcga atgcgccgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct
    11461 tccgcgccgg tttctgctgc gccactgctg caggtgagcg gcgcactcat cgccattatt
    11521 gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgtccc taaacgcact
    11581 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg
    11641 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    11701 ctgcataaac ttcccccttc agcaccaacg gaagagatac cgcagcccga ttttcagtcg
    11761 gttatgaaaa acttgcttaa gcgtagcggg agatcctgat gcgtcgtttg ttgtctgtcg
    11821 cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg ggtatcacca
    11881 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcaaacg ctggtgttca
    11941 tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca
    12001 tcattgtttt tggtttattg cgtaacgcgt tgggaacacc ctccgcgcca cctaaccagg
    12061 tattgctggg actggcgctg tttttgacct tttttattat gtcgccggtg atcgataaaa
    12121 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat ctcaatgcag gaggcgctgg
    12181 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgcgag gcggatttag
    12241 ggttgtttgc cagactggcg aatactggcc cgttgcaggg acctgaagct gtgccgatgc
    12301 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    12361 cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    12421 ggatgatgat ggttccccca gccacaattg ctctgccctt taaactgatg ctgtttgtac
    12481 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    12541 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    12601 cactggctgc cccactgttg ctggtggcgc tggtcacggg tcttatcatc agtattttgc
    12661 aggccgccac ccagattaac gaaatgacgc tgtcgtttat tccgaaaatc atcgccgtat
    12721 ttatcgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    12781 ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc aggtaacaag
    12841 cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct
    12901 catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct
    12961 ggcaatgatg atcacgttcg ctattgcccc atcattacct gccaacgatg ttcctgtttt
    13021 ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt
    13081 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggt gaaattatcg gtctgcaaat
    13141 ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc ccgttttagc
    13201 gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt
    13261 gatttcactg ctggccgata cctttcacac cctgccaatt ggtggcgaac cgttgaacag
    13321 caatgcgttt ctggcactca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct
    13381 ggcattaccg ctcattactc tgctgctgac cctgaatctg gcattaggct tacttaatcg
    13441 tatggccccg caattatcca tttttgttat tggatttcca ttaattctga ctgtcggtat
    13501 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    13561 gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt
    13621 ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct tccaggatac
    13681 tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttacttta
    13741 agacaattcc aggcaaatta tacaacactt tacgggatag taagtccgcc tgaaaaatcg
    13801 cgagagtggc gcattaggtg acccatgttg ttccgtttag tcatgatgaa atattcaggt
    13861 aaggggaatt atcgttacgc attgagtgag ggtatgccat gtcaacgatt attatggatt
    13921 tatgtagtta cacccgacta ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa
    13981 gagaaatcaa cgacattgaa accgttgatg accttgccat agcttgtgat tcacagcgcc
    14041 cttcagtggt gtttattaat gaggactgtt tcatccacga tgcttctaac agtcagcgta
    14101 tcaagctcat cattaatcaa catcccaata cgttatttat cgtttttatg gcaattgcca
    14161 atgttcattt tgatgaatat ctattggtca gaaaaaattt attgatcagt tctaaatcga
    14221 ttaaaccgga atctctcgac gatatccttg gcgatattct gaaaaaagag acaacgataa
    14281 cctcgttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa
    14341 tgtggatggc aggtcaggga accattcaaa tctctgacca aatgaatatc aaagccaaga
    14401 ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta
    14461 tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct
    14521 aacacattct gactggtggt ttcccaccag tcaggccgaa ttagattact ctgctttctc
    14581 cacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc
    14641 ttccagcgaa accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccacgcc
    14701 aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt
    14761 cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc
    14821 gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg
    14881 gccgcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agagtacagc
    14941 gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa
    15001 attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag cgtcgatgga
    15061 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    15121 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggggtcg
    15181 ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg
    15241 tgttcatcac cttatccgca atttttttcg ctgacaagaa aatattcgtg atatgaatga
    15301 ttaacgctac ttatgaatag aaatgtgacg cgcgtcaccc cttggtggct ctggcgtcaa
    15361 cgggatcgct cccgaaaaaa tcatctttga taatttgtgc tgttttgaga atcatcctgg
    15421 taaggggagt actgcgcaac catgctttca attcaacaac cactactggt ttttagcgat
    15481 cttgatggca ccctgctgga cagtcatagt tatgactggc aaccagctgc cccctggctc
    15541 agccgtttac gcgaagcgaa tgttcccgtc attctctgta gcagtaagac atcagccgaa
    15601 atgctgtact tgcaaaaaac gttggggcta caaggtttac cgctgattgc cgaaaatggc
    15661 gcagtgatcc agcttgctga gcaatggcag gatatagacg gttttccacg catcatctca
    15721 ggtattagcc atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa agaacatttt
    15781 aaattcacaa cttttgatga tgtcgacgat gcaaccatcg ccgaatggac gggattaagc
    15841 cgtagccagg cggcgctgac gcagctacat gaggcgtcgg taacgctaat ctggcgcgac
    15901 agtgacgagc gtatggcaca atttaccgct cgtctgaacg aactgggctt gcagtttatg
    15961 cagggtgcgc gcttctggca cgtcctggat gcctctgccg gaaaagatca ggctgccaac
    16021 tggattatcg cgacctatca acaatcgtca ggcaaacgcc caaccacact tggcctgggc
    16081 gatgggccaa acgatgcgcc cttactggag gtaatggatt acgcggtgat tgtgaaaggg
    16141 ttaaaccgtg aaggggtgca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag
    16201 cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc ccattaagcg
    16261 ttatcgctcg cgaatacccg attgcgcccg gcctgtttag cgagataaag tcgacggtcg
    16321 gccagtgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct gcttaccccc
    16381 agcgaggcac tgatgcgtat cgtcgtactc ttggcgatca acatctcttt ttcatttagc
    16441 ttcaggcgaa tacgttctgc aacctccgca gcctgcgtca ggctcgcgcc tggcagaatc
    16501 acacaaaact cctcaccacc gacccgcccg gcaacatcct gcgctcgcaa ggaactgcta
    16561 attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatgaccaaa gcggtcatta
    16621 atcgctttaa aatggtcaag gtcgacctga atgacagaaa aaggatgttg gtgcgtctga
    16681 cacaatttag cgagcggacg ggctttttcg aacagtgcgc cacggttata taaacgcgtt
    16741 aaggtgtcgt gccacgcctg ccagtgcaac gagctttgca aaacatacat gttgctgacc
    16801 atccggcgaa tcacatacca ggagatgagt aacatggtgg taaagagcgc ccacagcagg
    16861 gttaatgcaa tgctgatact gccgaaatcg ccgcgcacgc cttcgcttag cgtatggacg
    16921 cgcaccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg actgttcata
    16981 cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgagga
    17041 tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaataatct cagcttactg
    17101 tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc
    17161 atggtacgca cgggaatact catccccagc acgccatacc agtagttttc actgtctacc
    17221 ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg cgaagtgaac
    17281 cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgacaaacca gggttgggtg
    17341 acatagccgt aataacgcgt tggtacattg cgcgtaaaca aggtcggctg cgtcgaaacg
    17401 taaaatccgg cacgcgagac atacatcgcc tgttcaacca tcgacgagga gttgtgtgcc
    17461 agtcgcagca agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt
    17521 tcgcgagaca ggagattccc ctcgctgact aatgcatccg acacaccatt gactggcagg
    17581 gtgcgtcgtc ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcgaactcg
    17641 gttacggcat tacgcagaga ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca
    17701 ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct
    17761 acgttttcca gatgattacg ctggctggag atataggcat cttccagcac caccacttca
    17821 cgccaggtga gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgacatga
    17881 ccaggcccca ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg
    17941 tgctgcaccc tgacgactcc caacctgctt ttttatgatt ctggcataac ctggcgatag
    18001 cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga cagacatcga
    18061 tagttttata acgtaggcat gataagacgc gatagcgtcg catcaggcaa ttcagtgcag
    18121 acactggcac acccgaccgg atgcgacgtg agcgccacat ccggtcgggt gttaatgata
    18181 ccctttcaaa cttcttccgc agaacgactc ccctcccccg gaacaagctc ggtttcatga
    18241 aaagcttccc gcttgacccc gtaaccgtca taccaacgac actcaaccat accgctggag
    18301 tatccggtga caatcatccg cgggccgccc tctttaaccg taacttcctc actaaccata
    18361 aagctcatac tcgcctcctt ttttcgagtg aaacttgttc accttagttg aagatggcga
    18421 attttgcata atagcctgtg cgagtattaa tgtgcctgac cgcgtatttt cgctacggct
    18481 ttcactccca gcaccacgat gccgccgatg ataaatccaa gaatcagatt taaaacagtc
    18541 ggtaatatca tcgccaccac tgcactttgc tgcccggcga aatgttcaat ggcgtgatgc
    18601 agcggcgcaa ttccatgtac cacaatccca ccgccgacga ggaacatcgc cagcgtgccg
    18661 acaatcgata acgccttcat caaccagggc gcaatgatca ataacccttt gcctaatgcc
    18721 tgcatcagcg cgctggattt ttccgccagc caatacccca ggtcatcaat cttaacgata
    18781 acccctacca gaccgtagac gcccacggtc actaccagcg cgatgcctga aagcaccagc
    18841 acctgattaa gcaacggcgc ttcggccaca atccccagcg tgatggcgac gatttccgca
    18901 gacaatataa aatcggtacg aatcgcccct tttattttgt ctttttcaaa cttcagcgga
    18961 tcctgcgccg ccagcttctc cagacgctgc tgactctgcg ccggatcttc tttatgttta
    19021 cgcgcctcca gcatatgcag cactttctct accccttcaa agcagagaaa cgcgccgcca
    19081 atcatcaaca gtggcgtaat cgcccacggg ataaacgcac tgatgatcag cgccagcggc
    19141 accagaatca ctttattgat cagcgatcct ttcgccacgc cccagaccac gggaagttcc
    19201 cggttggcgc gcacgcctgc aacttgttgc gcattgagcg ataagtcatc ccctaataca
    19261 ccggcggttt tcttcgccgc cagtttgccc atcacggaga tatcgtccag cagtgtggcg
    19321 atatcatcga gcaacgtcag taaactactc ccggccaaaa gcactccctc tcatttttat
    19381 tgttgaaggg tgtaagtatg aagcaaaaac ggcagctctg atacttgctt cacaggtcaa
    19441 catttattta acaatcaagt gcaattatat ctctcgccag cagaatgatt ttcacgttta
    19501 ctatgaacca cctttttatt tcagccgtga gggattatgc gtttccgcca gttgttaccg
    19561 ctttttggcg cgctgtttgc gttgtatatc atttggggct caacctattt tgtcattcgg
    19621 attggcgtgg aaagctggcc tccgttaatg atggcgggcg ttcgattcct ggcagccggt
    19681 attttattgc tggcattttt gctactgcgc ggacacaaac ttcccccgct acgtccgctg
    19741 ctcaatgccg cgctgattgg cctgttattg ctggctgtcg gtaatggcat ggtgacggtt
    19801 gccgaacatc aaaatgttcc ttccggcatc gccgccgttg tggttgcaac cgtgcccctc
    19861 tttaccctgt gcttcagccg cctgtttggc attaaaacgc gcaaactgga atgggtgggt
    19921 atcgccattg ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt aagcggcaat
    19981 ccgtggggcg cgattctgat tttaatcggc tcgattagct gggcgtttgg ctcggtttat
    20041 ggctcgcgca ttaccttacc tgtagggatg atggcgggtg cgattgagat gctggcggct
    20101 ggcgtggtgt taatgattgc gtcgatgatt gcgggtgaaa aactgacggc gctcccttcc
    20161 ctttcaggct tccttgcggt cggctatctg gcgctgtttg gttcgattat cgccattaac
    20221 gcttatatgt atttaatccg caacgtcagt ccggctctcg ccaccagcta cgcctacgtt
    20281 aacccagtgg tcgcggtctt gctgggtacg gggctgggtg gcgaaacgct gtcgaagatt
    20341 gaatggctgg cgctcggcgt aattgttttc gcggtggtac tggtcacgtt gggaaaatat
    20401 ctcttcccgg ccaaacccgt tgtcgcgcca gttattccgg atgcatcaag cgagtaaatg
    20461 aatcccctgc gtgtcgatct gcgcgctggc accttcgccg cagatccact cttccagacg
    20521 ctcggtaagc gccgcatccg tcagcttctc gcgcccgcgt aacgcacatt cccagacaat
    20581 caatacgcgc caaccgagtt cctgcaaatg gctgatatca cgacgatcgc gctcgacatt
    20641 tttacctatc ttctccagcc aaaaatctgt tcgcgtggcg gggactttaa acaggtagca
    20701 atggtgatgg tgccagaagc aaccgtgggc aaatatcaca cagcgaaatt catcaaggac
    20761 aaaatccggg cgtccgggca gactggcgtc ctgaacgcga aacgccaggc cctgcccagt
    20821 taacaaactg gcgaggcgct tctcaatcgc cgtatcacgc gtggcaatcg cgcgcatatt
    20881 tttgctgcgg gtggccttat cgtgaacgtc gaccatgttg tgcctcttgc tgacgcaacg
    20941 ccaccgcctg tttgattttt ggctcaagca gttttgccac cgcggcaaag accggcacga
    21001 ccaccgagtt accgaactgg cgataagcct gagtgtccga aaccggaatg cggaatttcg
    21061 cttctcccgg cgcttcaaaa cccattaagc gcgcacattc ccgaggcgtt aaccgacgtg
    21121 ggcgatgttg ctgattcagc ggatcgtcaa agtctttctc acccgttgcc atatcccagc
    21181 cgcgatcgat taaaatttcc gcgccatctt tgtaataacg cgcagacagc gtacgcgtga
    21241 cgctttgcgg attgttcgga taaaccattc cataaccgaa gccgttaccg cgcgcctgat
    21301 gttttttcgc atatcgatag aggtacttcc acagtaccgg cgtcaggata tatttcgcct
    21361 cgaccatcgg gtccagcagc tgcgccagcg tcactcgctg tgcagggaaa catttgctga
    21421 tatcacgcag agtaaaatcg gctttcagat tcagatcgcg acgaaaaccc accagcacga
    21481 tgcgttcacg gtgctgcggc agaaaatgtt tgccgtcgat gattttcgga tcgtccggcc
    21541 cgttatcttc tgcatcagcc acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc
    21601 ggaacgtttt accctggtcg tgacttttca ggtttttgac gttttcgagc acaaacatcg
    21661 ccggacgacg cgcgtcgata atgcgcacca catcaaagaa cagcgtgccc tgggtatcgc
    21721 aggcaaaacc gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc agcgaaaatg
    21781 gctgacaagg gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt
    21841 ccgccgccgc ctcatcactc acgccttctt gatggctgag ggtgatgtcg cggatatcct
    21901 cattaaaatg atgcgtcgcc gggtcgcaat aatggttggc tttataagtg cgtaccgcat
    21961 gtttgttcca ttcgctggta aacacgcact gtccaccaat cgattcaaaa ccgcgacgga
    22021 tgccgccaat tccggcaaat agatcgataa agcgaaacgc ataatgcgga tgatgtgccg
    22081 gtggtttggg taacaacgtt tgcagatggg cgaactcttt ctcacttaaa cggtgccatg
    22141 ccgagtcatt cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc tcacccacac
    22201 cattaagctg cgccaccagc gttttaacat cataaatttg cagcagtttc tccagcattg
    22261 cctgtgcggc attcccggtg ctgtatgaat cggttactga tatattttcc tgcatagatt
    22321 taaccggcca tctgagatgg ccgacaggtt aacataatta ctgccgttta gggccacgcg
    22381 ctcagccagc aggtgaaaac gcatctatca ccttgtgatc gataccttca ttctcccccg
    22441 ccagttcggc accgagcttc gccataaact cgactaaaaa ttgcgcgttg tgctgtgcca
    22501 gctgcttgcc tcttgcggtt tgcatggttt gcggtaattt cagtaacttt gtctggaaat
    22561 gatccagcgc gtagcgttta tcatcaagcg ggcgatgctg tgcgaacgga tcttcgccat
    22621 caaacagcgc caagcccaat gcccctgaaa cggcaaatac gcgcgccagg ccaatcgccc
    22681 ccagggcttc cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga
    22741 tttgcgcgct gaaactgtga gcggcaatgg catgacaaac ggcctcaatt ttctccgccg
    22801 gaaattgcac gaact
//
LOCUS       DAZKGS010000065        22476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKGS010000065 DAZKGS010000000
VERSION     DAZKGS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..2280)
                     /gene="relA"
                     /locus_tag="ACHOQY_002519"
     CDS             complement(46..2280)
                     /gene="relA"
                     /locus_tag="ACHOQY_002519"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9678600.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(2328..3629)
                     /gene="rlmD"
                     /locus_tag="ACHOQY_002520"
     CDS             complement(2328..3629)
                     /gene="rlmD"
                     /locus_tag="ACHOQY_002520"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9678601.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            3686..6442
                     /gene="barA"
                     /locus_tag="ACHOQY_002521"
     CDS             3686..6442
                     /gene="barA"
                     /locus_tag="ACHOQY_002521"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9678602.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(6674..8014)
                     /gene="gudD"
                     /locus_tag="ACHOQY_002522"
     CDS             complement(6674..8014)
                     /gene="gudD"
                     /locus_tag="ACHOQY_002522"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9678603.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(8035..9375)
                     /gene="gudX"
                     /locus_tag="ACHOQY_002523"
     CDS             complement(8035..9375)
                     /gene="gudX"
                     /locus_tag="ACHOQY_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP9678604.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(9377..10729)
                     /gene="gudP"
                     /locus_tag="ACHOQY_002524"
     CDS             complement(9377..10729)
                     /gene="gudP"
                     /locus_tag="ACHOQY_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9678605.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(11164..11613)
                     /gene="yqcA"
                     /locus_tag="ACHOQY_002525"
     CDS             complement(11164..11613)
                     /gene="yqcA"
                     /locus_tag="ACHOQY_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9678606.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(11631..12413)
                     /gene="truC"
                     /locus_tag="ACHOQY_002526"
     CDS             complement(11631..12413)
                     /gene="truC"
                     /locus_tag="ACHOQY_002526"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9678607.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(12413..12742)
                     /gene="yqcC"
                     /locus_tag="ACHOQY_002527"
     CDS             complement(12413..12742)
                     /gene="yqcC"
                     /locus_tag="ACHOQY_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9678608.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(13364..13909)
                     /gene="syd"
                     /locus_tag="ACHOQY_002528"
     CDS             complement(13364..13909)
                     /gene="syd"
                     /locus_tag="ACHOQY_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9678609.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            13977..14825
                     /gene="queF"
                     /locus_tag="ACHOQY_002529"
     CDS             13977..14825
                     /gene="queF"
                     /locus_tag="ACHOQY_002529"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9678610.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            14937..16301
                     /gene="ppnN"
                     /locus_tag="ACHOQY_002530"
     CDS             14937..16301
                     /gene="ppnN"
                     /locus_tag="ACHOQY_002530"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9678611.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            16858..18147
                     /gene="sdaC"
                     /locus_tag="ACHOQY_002531"
     CDS             16858..18147
                     /gene="sdaC"
                     /locus_tag="ACHOQY_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9678612.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            18205..19572
                     /gene="sdaB"
                     /locus_tag="ACHOQY_002532"
     CDS             18205..19572
                     /gene="sdaB"
                     /locus_tag="ACHOQY_002532"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9678613.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            19684..20439
                     /gene="xni"
                     /locus_tag="ACHOQY_002533"
     CDS             19684..20439
                     /gene="xni"
                     /locus_tag="ACHOQY_002533"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9678614.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(20494..21642)
                     /gene="fucO"
                     /locus_tag="ACHOQY_002534"
     CDS             complement(20494..21642)
                     /gene="fucO"
                     /locus_tag="ACHOQY_002534"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9678615.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(21670..22317)
                     /gene="fucA"
                     /locus_tag="ACHOQY_002535"
     CDS             complement(21670..22317)
                     /gene="fucA"
                     /locus_tag="ACHOQY_002535"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9678616.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         5451 a         6058 c         5439 g         5528 t
ORIGIN      
        1 tacagtatat atcaatctac attgtagata cgagcaaatt tcggcctaac tcccgtgcaa
       61 ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc gccccagcac
      121 ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt tggtgtcgct
      181 acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg tgatatcacg
      241 taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc cggcggagta
      301 gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca gttccgccag
      361 ttgttcgcaa tcggcgcggt gtactgaaat accgcgcccc tgggtaatga agccgacaat
      421 ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt tgccaacacc
      481 ttcgaccacc acgcgaccgt tatctttact gcggttttgc ggcgtgtagc ttttttgctg
      541 aagttgcttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa attgcgattg
      601 caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg ccagcaactc
      661 gtcgacatca ttaaagttgt aacgcggcag cagatgtttt tctgcttctt tcaggctgat
      721 ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt ttttgtcacg
      781 gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg tgacgtaacc
      841 gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga taatttcaat
      901 ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa tttttgcccc
      961 gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg gcgttgatcc
     1021 cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca tacacccggt cgtcaaagac
     1081 ctggctgcgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct gccacgcaat
     1141 cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc cagctgccgc
     1201 gccctcttta tatttccagt gcgcagcaac acccaactct gcatcttcat gcatctgttt
     1261 ggtgcggatt tggatctcaa cggttttgcc acctggcccc agaaccacgg tatgaataga
     1321 ctgataaccg tttggtttcg ggttagcgac ataatcgtca aactcatccg gcaggtggcg
     1381 atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct cggcgacaat
     1441 acgtaccgca cgcacatcaa acagctcatc gaaggcgagg ttctttttct gcattttgcg
     1501 ccagatactg tagatgtgtt tcggacgacc atacacttca gctttaacgc cttcagcttt
     1561 catctcagcg cgcagatggc cgacaaactc ttcgatatag tgttcgcggt cgagacggcg
     1621 ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg tggagataac ggaagcagta
     1681 atcttccagc tcccatttca gttgcccaat cccaagacgg ttcgccaacg gcgcgtagat
     1741 attggtgcac tcttttgccg ccagtacgcg ttcatcttcc ggcgcatctt ttacttcgcg
     1801 cagatgagca atacgctccg ccagtttgat gaccacgcag cgaaaatcat cgaccatcgc
     1861 caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag tgtgcgtcgc
     1921 tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa cgaccgactt
     1981 accgacgctc tcacgcagca catcttcgct gactacgttg gcatcagcca gagggaacag
     2041 cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct ccaccatctc
     2101 aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt gcagacaata
     2161 cgcccaggtt tcggctaagc actcacacga cttctggctg gtaataccca gacttgcgat
     2221 ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta ccgcaaccat
     2281 tgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgtaacta tttaacgcgc
     2341 gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag catcgccagt
     2401 cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag cgttgcaggg
     2461 ttacaggata cataaactat acgaataggt tccagtttta taatttgctg cataacacct
     2521 gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt cgcccacggc
     2581 tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg taaaccatta
     2641 agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac accgaccaca
     2701 ctggctgctt gtttcgccaa tggcagtgta aagttgccca taccgcagaa cagatccagt
     2761 acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat tttttggttc
     2821 acacccgcat tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa cccgtttgag
     2881 tcataccagg gcatctcacc agagacggtt tcgagtatct cactatcggg ggcgagatac
     2941 agatccaggc cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc cgaacttagc
     3001 ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag ttcaacatga
     3061 ccaagatggc gcataacttg taagctgccc aggcatgccc tgactttggg cagcaatgct
     3121 tcaagttggg gcactaaaat ggggcattgt ttaacgtcga caatgtcact ggagcccgct
     3181 ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt agttcagact taaacgcgcg
     3241 cggcggcgat agccccaggg aacatcggcg atcacttcag agacatcgtg tttcattaat
     3301 cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg ttgttgctga
     3361 cagccgccgc atacgccaaa atgaggacag cgtggtgttt cgcgttccgg gctatcgctt
     3421 aaccggcgta cgactttagc gcgggcatac tgttttttat cttcagtaac agcaacttcc
     3481 gcgttttcct gcggcaataa tccggggata aataacgttt tgccgttatg tcgcgccacg
     3541 ccctgaccaa aagagtcgag gtcgttgact gaaacggtta tgatctgacg cgtcgtcgtg
     3601 cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt tctcaattta acagtgtgac
     3661 cttaattgtc ccataacgga actccatgac caactacagc ctgcgcgcac gcatgatgat
     3721 tctgatcctg gcaccgaccg tccttattgg tttattgctg agtatctttt tcgtcgtgca
     3781 tcgctataac gacttgcagc gtcaactgga agatgccggt gccagcatta ttgagccgct
     3841 tgcagtttct actgaatatg gcatgagcct gcaaaatcgc gaatctatcg gtcagttaat
     3901 aagcgtactg catcgtcgcc attccgatat tgttcgcgcg atttcggttt atgatgaaaa
     3961 taaccgactc tttgtcacct ccaattttca tcttgatccc tcatcaatgc agctcggcag
     4021 caacgtgccg tttcctcgcc agctcactgt cactcgtgac ggcgatatta tgatcctccg
     4081 cacgccgatt atttctgaga gttactcccc cgacgaatcg cccagtagcg atgccaaaaa
     4141 tagtcaaaat atgttgggat atattgcgct ggagctggat cttaaatcgg ttcgcttgca
     4201 gcaatataaa gagatcttta tttccagcgt gatgatgctg ttttgtatcg gtattgcgct
     4261 tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca tggtgaatac
     4321 cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg gaaggattta tgctcggcga
     4381 gctggatatg ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg cttatcacga
     4441 agagatgcag cacaatatcg accaggcgac gtccgatctg cgtgaaacgc tggagcagat
     4501 ggaaattcag aacgttgagt tagatctggc gaaaaagcgc gcccaggaag cggcgcgtat
     4561 taaatccgag tttctggcaa atatgtcaca cgagctgcgt acaccactga atggtgttat
     4621 tggctttacc cgcctgacgc tgaaaacaga attaacacca acgcagcgcg atcacctgaa
     4681 tacgattgaa cgttcggcaa ataatttgct ggcaattatt aatgatgttc tcgacttctc
     4741 gaaactggaa gcaggtaagc tgattctgga aagtattcca ttcccactac gcagcacgct
     4801 ggatgaagtc gttactctgc tggcacattc ttctcacgat aaagggttgg aactgacgct
     4861 caatattaaa agcgacgtgc ctgataacgt gatcggcgac ccactgcgat tacagcaaat
     4921 catcactaac ctggtgggga atgcaattaa attcaccgag aatggcaaca ttgatattct
     4981 ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga ttcgggatac
     5041 cggcattggt attcctgaac gcgatcaatc gcgcttattc caggccttcc gacaggctga
     5101 tgccagtatt tcccgccgtc atggtggcac cggtctgggg ctggtgatta cacaaaaact
     5161 ggttaatgaa atgggcggcg atatttcgtt ccatagccag ccgaatcgcg gttcaacttt
     5221 ctggttccac attaatctcg atctgaaccc gaacattatt atcgaagggc catccaccca
     5281 gtgcctcgcc ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag cgcaatgcac
     5341 gctggatatt ttaagtgaaa cgccgctgga agtggtttat agcccaacgt tctccgcgct
     5401 gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc gcgagccgct
     5461 aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg accgatttcc tgatgctggc
     5521 acttccttgc catgcacaag tcaatgcaga aaaactcaag caagatggta tcggcgcgtg
     5581 tctgctgaaa ccattaacac ctacgcgcct gttgcctgcc ctgacggaat tttgtcatca
     5641 caaacaaaac acgcttttgc ctgtaaccga tgaaagcaag ctggcaatga cagtcatggc
     5701 agttgatgac aaccccgcta acctgaaact tatcggcgca ttgctggaag atatggtgca
     5761 acatgtggaa ctttgcgata gcgggcatca ggcggttgaa cgggcgaaac agatgccgtt
     5821 cgatttgatc ttaatggata ttcaaatgcc tgacatggat ggcattcggg cctgcgagct
     5881 catccaccag ctcccccatc agcaacaaac gccggttatc gcggtaacgg cgcatgcaat
     5941 ggccgggcaa aaagagaagc tgcttggcgc agggatgagc gattatctgg cgaaaccgat
     6001 tgaagaagag cgattgcata atttgttgtt gcgctacaag cctggcagcg gtatttcctc
     6061 tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga ccctcgactg
     6121 gcaactggca ctacgccagg cagcaggaaa aaccgattta gcgcgcgata tgctgcaaat
     6181 gttactcgat ttcctgcctg aagttcgcaa caaagttgag gaacagctgg ttggagaaaa
     6241 cccggaaggc ctggttgatt tgattcataa actgcatggc agttgcggct atagcggtgt
     6301 gccgcgtatg aagaatctct gccaactgat cgaacaacag ctacgtagcg gtactaaaga
     6361 agaagatttg gagccggagc tgctggaact gttggacgag atggataatg tagcgcgcga
     6421 agccagcaaa attctcgggt aacggacgtc ttgtcgagta gcaatcggct ttttatgacg
     6481 ctggtttcag attggtgaca aagtgcctgt gtttatgccg gatgcggcgt taacgcctta
     6541 tccggcctac aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac ctgatacata
     6601 gcacaacagg ctattttgcg tttagcatca gtctcaagcc ggctccagat agagccggtt
     6661 ttggttttct gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc cagccaggaa
     6721 tcagatactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc tgatacagct
     6781 catgggcttt catcacttga tccatatcga tttctacacc cagccccggt ttttccggca
     6841 cctgaaccag accgcctttg atctcaaacg gttctttggt caggcgctga ttaccttcct
     6901 gccagatcca gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg gcaacatggg
     6961 taaacatcgc cagggaaata tcgaagtggt tgtttgagtg tgaaccccag gtcaggccaa
     7021 attcatggca catttgcgcc acacgtaccg aaccttgcat cgtccagaaa tgcggatccg
     7081 ccagcgggat atcaacggat tgcagggaga gcgtatggcc catctgccgc cagtcggtgg
     7141 cgatcatatt ggttgcggtc ggcaggccag ttgcgcgacg gaactcagcc atcacttcac
     7201 gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagc gaccctttca
     7261 ggtatttacc aattttaatc gcttcgttca gcgaccaggc accgttagga tcgagcgtaa
     7321 tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga ctcggcctct tcttccccgg
     7381 ccagtacacc gcctttcagt ttgaaatcgt tgaagccata tttttcatac gccgcttccg
     7441 ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga taccagtcgc
     7501 atgagtcatc cggctggctt tgatacggca gcggtgtggc tttgcgatca ccgacgaaga
     7561 acagataacc gagcatttcg acttcgctac gctgttgacc atcgcccagc agcgatgcca
     7621 cgtttacccc cagatgctgc cccagcagat ccagcattgc cgcttctatc ccggtaacta
     7681 catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccagca tcacgatcgg
     7741 caaaagtatt acgcaccagc gtcagaacgt ttttgtattt acccagcgtt ttacctacca
     7801 ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga atttccccta
     7861 cgccagtgtg accagaatta tctttgataa tcacaatatt acgcgtaaag aacggtgcgt
     7921 gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg acctgcattt
     7981 cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc agaatcaatg
     8041 acggccgaaa acgggacgtt tacggtcaaa ggtccagccg gggatcaggt actgcatcgg
     8101 acctgcgtcg ttacgcgcac cgccaggcag acgtttatag gcctcatgtg ccttttgtac
     8161 ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa tttttccgtt
     8221 tttaatctcc agcggatttt tggtcaggcg acaatcgccc tcctgccaaa tccagtgggt
     8281 atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca tcgccagaga
     8341 gatatcgaaa tggttattag aatggcagcc ccaggtcagc ccccagtcgt cgcaaagctg
     8401 cgccacacgg actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg gaatatctac
     8461 cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcgatca tgttagtcgc
     8521 gacgggcaag ccggtcgccc gccgaaactc cgccatcact tcacgaccgg agaaaccctg
     8581 ttctgcgccg catggatctt cggcataggt aagaacatca ttcagccctt tgcatagaga
     8641 aatggcttca tcaagtagcc aggcaccgtt gggatcaacg gtaatccgcg catcagggaa
     8701 gcgtttcttc aatgcccgaa cagtgtcgat ttcttgctcg ccaggtaaca ccccgccctt
     8761 aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac gcacaacggc
     8821 ttcgctgttc atcgctttct gatggcgcaa ctgataccac tcatggttgc ccggcgtatt
     8881 ttccagataa ggaagatcgg ttttggtccg atcaccgata taaaacagat aaccgaggac
     8941 ggtaatagtc tcgcgttgct tgcctggccc caacagttcg cagaccggaa cattcagcgc
     9001 cttacctagc aggtcaagca aggcggcttc cagcgccgcc acggcattaa cgcgcaattc
     9061 aaaagtccag gcacctttgc cgaaggtatc aaaatcggct gcctgattac ctttatgcac
     9121 ctgctgaacc actttattca ggcgcgcaac ttcctggccc agaaccatcg gaatagcatc
     9181 gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg tatgcccggc
     9241 gttatcggtg agtaccacaa tattgcgggt gaaatatgcg ttatgtgccc caccaatatt
     9301 aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc atatcagtaa taacaggact
     9361 ggattgtgtc gtcatcttat tgccccgcga caggtttcag ctcgatacgc ttgatatcgc
     9421 ccaccagcac caggtagctc agtaccgcga ttaaggcatg aacaccaaca taaatcagcg
     9481 ccccattaaa ggagccagtc gtgccaacga tataaccaat tgcgattggc gtgacgatgc
     9541 cagaaatgtt accgaacatg ttgaacaggc caccggaaag accgctgatc tcttttggcg
     9601 cggtatctgc cattactgcc caacccagcg ccccgatgcc cttaccgaag aaggccagcg
     9661 ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc atcaccatcg
     9721 acagcaacat gcccattacg atcggtgttt tacgcgcaat gttcagcgat cccgtgcggc
     9781 gcatcagcca gtcggaaata atcccaccca gcacaccgcc gataaaaccg caaaccgccg
     9841 gaacggaagc cacaaagccc gctttcagaa tcgacatccc acgcgcctgc accagataaa
     9901 ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat acagtactga ccgatataaa
     9961 cgccgatcat catccgtgac ccgagcagtt gtttgatctg cccccacttc acgctgaacg
    10021 gcactttaac tttggtgttt tgctgatcca tattgatcag cgcgccaccc gcggcgatgt
    10081 actccagctc tttctgattt acccccggat gttgatttgg ctcgtggatg actttcaacc
    10141 agataaagct gatgacaatc cccagaccgc ccataaagaa gaagacgtgt gaccagccca
    10201 cttcatgcgt cagccagccc ataatcggcg cgaagatcac cgttgcgaag tattgagcgg
    10261 agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc gcaacaatgc
    10321 gactgttgcc ggggaaagat ggcgcttcag caagcccgac caggaagcgc agcgtaaaca
    10381 gggcaacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc aaggtaaaca
    10441 tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacga tccagcaacc
    10501 aaccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac acatagccca
    10561 ttcccacggg atcaaggccg atatctttgg ccatttccga accagcaata gaaagcgtag
    10621 cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc cagtaacgag
    10681 cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt gttgcactcc
    10741 tgaaaattcg cgttagccac gctcactctg gactgcgaca tcgccaggaa atcagaggtg
    10801 acgtagggtg ttttttgccg tttttatagg tcgttcgccg aatacggcgc gtgtttatat
    10861 ctggcaatag cagtataaaa agcgcgccat agcggctcac cgtgcaacaa cacaacatta
    10921 atgcgttcaa tgaggcccga ttttggcatt agccctggac ggtggaatcc acttcacgga
    10981 aatgaaaaca agaacaagaa aggaagggta aaaacgaaga aataaagaag agtatgaaat
    11041 ggatcgcttg actccaggca aacgccagta aaaatccgcg ctatgaagca gtttttactg
    11101 gcatttgcct gaaaagattc gattcagcac cgctaaaacg acatttaccg ctcgctgaac
    11161 atatcaggac aacagcgtgc cccactgttc gacccacgga tttgattccg tttccggttc
    11221 cgggttctca ctggcgtcaa tcaacagtat ttcgccaacc cgctgagcgc tctgttcctg
    11281 caacaaggca tcgaactgtt tgccgccatt gcagaaattt acataactgc tgtcgccgag
    11341 cgcaatcacg ccataacgca gattcggctg gaagcccaga ctatctttga ttccctgaaa
    11401 gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac
    11461 atacttatcc tgatagggca gccagtcgct taattcagga tcttcaaata ccgttgcttt
    11521 gtggccctgt gcggtcagaa ttgcttctgc ttcttccgcc actaacagcg aatttccgta
    11581 catggtgccg acaaaaatac caatttccgc cataccgttt tctccctgga ttaggaactt
    11641 atctctccat cctgacccga tggcgcactg aactcaaccc tttcattttc aggaagcaga
    11701 ccgcgccagc caaattgtga taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata
    11761 gtcagcggct cgccagtaaa aggatgcgtc aatgacaact gactggcatg tagcattaac
    11821 cgctggaggc caaaatgctc agcaccgctg cgattctggc gtaaatcgcc atgtttgcta
    11881 tcgccaataa tcggatgacg caaatgggca agatgtcggc gaagctgatg tttgcgtccg
    11941 gttttcggct ccagttccac caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc
    12001 ggcatttcta cggtcgccag accgcgataa tgcgtcactg ctggctgcga gcctttatct
    12061 tcgcgggcaa atttatcagc gattttgtcc agttcttcca ccagtggata atccagaact
    12121 gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac gtttctggat ttggtgctgt
    12181 tcaaactgtt gtgccagcag ccgtccggcc tcgctggata atcccatcaa caacacacca
    12241 gaagtgggtc ggtccagacg atgagcggta aaaacgtgct ggccaatctg atcacgcacg
    12301 gtttgcatga ccactacttt ctcgtcgcga tccagccagc tgcggtgaac cagccagccg
    12361 gagggtttat ttaccgcaac cagccattca tcctgataga gtatttccag cattagctcg
    12421 catcatccgc aaaaagagca tccagttttt ccagctcagc cagaataagc gcgcgttgcg
    12481 gatggtccgt cgccagcgcc atttcataat agggtgcaac ggcaaaagcg cccggtaacg
    12541 gctgtttgtt atctaacaaa tcgtgcattc gcgggatcag cacccactgc aaccactcca
    12601 gtggttccat ggtgtccata aagaacggtt gggtactatt aaattgatgc ggctggggtt
    12661 catcgtttcg ccagtgctga tgttcacgca gtaacgcttc aagcgcctgc aactggagac
    12721 gaacgcggtc atgagtggtc ataaagcaac ctcaataaga aaaactgccg cgaaggatag
    12781 caggaataaa aaaagggagc actgtattca cagcgctccc ggttcgtttc gcagcattcc
    12841 agctactttt gttgctccct gctcatcctt gacaactttt cctctggcct tgcggccaat
    12901 cgttcatcct gaactattgc ttcctgctca caccaccccg atgtgatact tcatcctgaa
    12961 gtgtccctgg ccttcctgac ccaccgaatc atcctgaccg gttctcattc tccatcctgg
    13021 aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc atccagaagc ctttccctga
    13081 aacaccatcc tggtgtgtcc tgcagaagtg tcatcatcct gatgttcact tcgttgtctg
    13141 actccctgtc gacgaagata gaatcgtctt tttcgcgaag tcttacaagg cgcttacaga
    13201 caatgcctta aggaaaattt catatgaaag ttaagaatta acaatcaaca ttttgatttt
    13261 aaatgaaaat aacgattctt atctttttta gtattcgctt atttcctggc gatctcctac
    13321 agagtctgta agagatctct cacaaccaga tggggtattt ggattacaga agaggcttaa
    13381 gttgattaag gaattccgca agatttgagg ctaaatgcgt ccgtttacgt gttcccagcg
    13441 tctctttgca cacttcaccg gagagattac acaccgagat gacctctaac tcttcttcca
    13501 gcgtcgcgat aaatagcgtg ggcggcagtt ttagcctttt ctgagtcacc agatgaccaa
    13561 tcagattttc ctgcacccga cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt
    13621 tgatatcgcc aaactgcgcg tgcatatccc cggcaaactg agtcgtataa aaggtatgaa
    13681 ttgtgggttg tatcacaata tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg
    13741 taaacggctg gggttgccag tatacggcat cttcggtagt tgaaataatg cacggtgaag
    13801 gaacaccgta cagttcctca cttaacggcc agcttttatg ctcttcatgc cacgcatcgc
    13861 agtagcgtgc agtaaaatct ttcagggctt gtgcggtcaa atcgtccact aatttctctc
    13921 ttcacttaaa ccagatacac ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt
    13981 cttcttatgc aaaccatcag gcacttgcgg gcctgactct tggaaaatca accgattacc
    14041 gggataccta tgacgccaac ctactgcaag gcgttccacg cagcctgaat cgcgacccgc
    14101 tgggtctgaa agcggataac ctgccttttc acggtacgga tatctggacg ctgtatgaac
    14161 tttcctggct gaatgcgaaa ggtttgccgc aggttgccgt cggtcacgtt gaacttgatt
    14221 acaccagcgc aaatctgatt gagtcgaaga gttttaagct ctatctcaac agttttaacc
    14281 agacgcgttt taataactgg gatgaggtgc gccagacgct ggagcgcgac ttaagcactt
    14341 gcgcccaggg taaggttagc gtggcgttat atcgtcttga tgaactggaa ggccagccga
    14401 taggtcattt caatggcacc tgcattgatg accaggatat cactatcgat aactatgaat
    14461 tcactactga ctatctggag aatgccacca gcggtgaaaa agtagtggaa gagacgcttg
    14521 tcagccacct gctgaaatca aactgcctga tcacccatca accagattgg ggttcgatcc
    14581 aaattcagta tcgtggacgc caaattgaca gagaaaaact gctgcgttac ctggtctcat
    14641 tccgtcatca caacgagttc cacgaacagt gcgtggaacg catctttaat gacctgttac
    14701 gcttctgcca gccagagaaa ttaagcgttt acgcacgtta tacccgtcgt ggcggtctgg
    14761 acattaaccc gtggcgcagt aatagcgatt ttgtcccatc gaccacaaga ctggttcggc
    14821 aataaatttt ttctcaattt tgcgtgctgg attcacgcag aaggttgtga aaggtcatca
    14881 ggcagggcta ttgtaatcaa agggaatgac gatattcgtc ccataaggag tttttcttga
    14941 ttacacatat tagcccgctt ggctccatgg atatgttgtc gcagctggaa gtggatatgc
    15001 ttaaacgcac cgccagcagc gacctctatc aactgtttcg caactgttca cttgccgtac
    15061 tgaactccgg tagtttgacc gataacagca aagaattgct gtctcgtttt gaaaatttcg
    15121 atattaacgt cctgcgccgt gaacgcggcg taaagctgga actgattaat cccccggaag
    15181 aggcttttgt cgatgggcga attattcgcg ctttgcaggc caacttgttc gcggttctgc
    15241 gagacattct cttcgtttac gggcaaatcc ataataccgt tcgttttccc aacctgaatc
    15301 tcgacaactc cgtccacatc actaacctgg tcttttccat cttgcgtaac gctcgcgcgc
    15361 tgcatgtggg tgaagcgcca aatatggtgg tctgctgggg cggtcactca attaacgaaa
    15421 acgagtattt gtatgcccgt cgcgtcggaa accagctggg cctgcgtgag ctgaatatct
    15481 gcaccggctg tggtccggga gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg
    15541 cgcagcagcg ttacaaagac agtcgtttta ttggtatgac cgagccgtcg attatcgccg
    15601 ctgaaccgcc taacccgctg gtcaacgaat tgatcatcat gccagatatc gaaaaacgtc
    15661 tggaagcgtt tgtccgtatc gctcacggta tcattatctt ccctggcggt gtgggtacgg
    15721 cagaagagtt gctgtatttg ctgggaattt taatgaaccc ggccaacaaa gatcaggttt
    15781 taccattgat cctcaccggc ccgaaagaga gcgccgacta cttccgcgta ctggacgagt
    15841 ttgtcgtaca tacgctgggc gaaaacgcgc gccgccatta ccgcatcatc attgatgacg
    15901 ccgctgaagt cgctcgtcag atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgcg
    15961 acactggtga cgcttacagc tttaactggt caatgcgcat tgcaccagat ttgcaaatgc
    16021 cgtttgagcc gtctcacgag aatatggcta atctgaagct ttacccggat caacctgttg
    16081 aagtgctggc tgccgacctg cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag
    16141 aagtcggtat tcgcgccatt gaagagtttg gtccttacaa aatcaacggc gataaagaga
    16201 ttatgcgtcg tatggacgac ctgctacagg gttttgttgc ccagcatcgt atgaagttgc
    16261 caggctcagc ctacatccct tgctacgaaa tctgcacgta atctcccctc ccccggtgac
    16321 gtttgttgcc gggttttctc gtttttggtc acttactcat caactcattt catttgttat
    16381 atgaatgttt cttaccaccc tcacgcgaca aatatcatca cagttaatat gtcatacaat
    16441 ttatattgca acgcaaacgt ttccctattt tcataaaccg tattttatcg ctatgaaaaa
    16501 gaaattatcg ccatgattaa ctaaaagtat tgattttttc agttcaacct acatatattg
    16561 cgcgccccgg aagaagtcag atgtcgttta atgggcaaat attgccctta aattctcttt
    16621 tacttttgat ttacagagta aagcgttggg ataatctatc ttccaagtag attattgtat
    16681 ttgggatcaa gatcactgat agatacataa cttgtgtgta tctttccgcc ctcaaattat
    16741 tacggcggta aatgattaag ccatcgccga tagacagatt tcatttttac ggtcaggcac
    16801 cttcccgggc tgaactggct aaaagctgaa ttatttgcat tcctccagga gaaatagatg
    16861 gaaacgactc aaaccagcac gattgcgtcg aaagactctc gtagtgcctg gcgcaagaca
    16921 gacaccatgt ggatgctggg cctttacggc acggcaatcg gcgcgggcgt gctgttcctg
    16981 ccaatcaacg ccggtgttgg cggtatgatc ccgctgatca tcatggctat ccttgcgttc
    17041 ccgatgacgt tttttgctca ccgcggcctg actcgcttcg tactgtctgg taaaaacccg
    17101 ggcgaagaca tcaccgaggt tgtagaagaa cactttggta ttggcgcagg taaactgatt
    17161 accctgctct acttcttcgc tatctacccg atcctgctgg tttatagcgt ggcaatcacc
    17221 aatacagttg aaagcttcat gtctcaccag ctgggtatga cgccaccgcc gcgtgcgatt
    17281 ctgtcgctga tcctgatcgt gggtatgatg accatcgttc gctttggtga gcagatgatc
    17341 gttaaagcga tgagtattct ggtattcccg tttgttggcg tactgatgct gctggctctg
    17401 tacctgatcc cgcagtggaa cggcgcagca ctggaaacgc tgtctctgga cactgcatct
    17461 gcaaccggaa acggtctgtg gatgaccctg tggctggcaa ttccggtaat ggtgttctcg
    17521 ttcaaccact ctccgatcat ctcttctttc gccgttgcga agcgtgaaga gtacggcgat
    17581 atggcagaac agaaatgctc gaagatcctg gcattcgcac acatcatgat ggtgctgacc
    17641 gtaatgttct tcgtcttcag ctgcgtactg agcctgactc cggcagacct ggctgcggct
    17701 aaagagcaga acatctcgat tctgtcttac ctagctaacc actttaacgc accggttatc
    17761 gcgtggatgg ctccgattat cgcgattatc gctatcacca aatccttcct cggccactac
    17821 ctgggcgcac gtgaaggctt caacggtatg gtgattaaat ctctgcgtgg taaaggtaag
    17881 tctatcgaaa tcaacaagct gaaccgtatc actgcgctgt tcatgctggt aacgacctgg
    17941 attgttgcca ccctgaaccc gagcatcctg ggtatgattg aaaccctggg cggcccaatc
    18001 atcgcgatga tcctgttcct gatgccgatg tacgcaattc agaaagtacc ggcaatgcgt
    18061 aagtacagcg gtcacatcag caacgtattc gttgtcgtga tgggtctgat tgcaatctcc
    18121 gcaatcttct actctctgtt cagctaagtc ctttcgcgcc gctttcgggc ggcgcttcct
    18181 ccgttttaac gcgatgtatt tcctatgatt agcgtattcg atattttcaa aatcggcatt
    18241 ggcccttcca gttctcatac cgttggacca atgaaagcgg gtaaacaatt taccgacgat
    18301 ctgattgccc gtaacctgct taaagacgtg acccgcgtgg tggttgacgt gtacggctcg
    18361 ctctctctga ctggtaaagg ccaccacact gatatcgcca ttattatggg cctggcgggt
    18421 aacctgccgg ataccgtgga tatcgattcc atccctggtt ttattcagga tgtgaatact
    18481 catggtcgcc tgatgctggc aaacggtcag catgaagtgg agttcccggt tgatcagtgc
    18541 atgaacttcc atgcagacaa cctttctctg catgaaaacg gtatgcgcat taccgcgctg
    18601 gcgggcgata aagtcgttta cagccagact tactactcta ttggcggtgg ctttatcgtt
    18661 gatgaagagc attttggcca gcagaatagc gcaccggttg aagttcctta tccgtacagt
    18721 tcagcagccg atctgcaaaa acattgtcag gaaaccgggc tgtcactctc tggcctgatg
    18781 atgaaaaatg agctggcgct gcacagcaaa gaagagctgg aacagcacct ggcgaacgta
    18841 tgggaagtca tgcgtggcgg tattgagcgc ggtatttcca ccgaaggcgt gttgcctggc
    18901 aaactgcgcg ttccacgccg tgctgcggca ctacgccgga tgctggtcag ccaggataaa
    18961 accaccactg acccgatggc ggttgttgac tggatcaaca tgtttgcatt ggcagtgaac
    19021 gaagagaacg ctgctggcgg acgcgtggtg actgcgccga ctaacggtgc gtgcgggatt
    19081 atcccggcag tgctggcgta ctacgacaag tttatccgcg aagtgaacgc taactcactg
    19141 gctcgttacc tgctggtagc cagtgccatt ggttctcttt ataagatgaa cgcgtcaatt
    19201 tctggtgctg aagtcggctg ccagggtgaa gttggtgtgg cgtgctcaat ggcggcggct
    19261 ggtctggcag aactgttagg tgcaagcccg gcgcaggtgt gcatcgcggc ggaaatcgcc
    19321 atggagcaca acctcggtct gacgtgtgac ccggtcgccg gacaggtcca ggtgccatgc
    19381 atcgagcgta acgccattgc ggcagtaaaa gcggtgaacg ccgcacgtat ggcgctacgc
    19441 cgtaccagcg agccgcgcgt ctgcctcgat aaagttatcg aaaccatgta cgaaacaggt
    19501 aaagatatga acgccaagta ccgcgaaacc tctcgcggcg gcctggcaat gaagatcgtt
    19561 gcctgcgatt aatcgctctc cagaggcctc gttttgcgag gcctcttccc gatttctcat
    19621 ccagccgtag cctgttccgg catcgaatgt tacccttatc gcctgatctt taagggggtt
    19681 atcgtggctg ttcatttgct tattgtcgat gcactgaatc ttattcgtcg cattcatgcc
    19741 gttcagggat cgccctgtgt cgaaacctgc cagcatgcgc tcgatcagct cattatgcac
    19801 agccagccaa cccacgcggt cgccgttttt gatgatgaaa accgcagtag cggctggcgt
    19861 catcagcgtt taccagatta caaagcgggt cgaccgccaa tgccggaaga gttgcacgac
    19921 gagatgcctg cattacgcgc cgcctttgag caacgcggcg tcccgtgctg gtcaaccagc
    19981 ggcaacgaag ccgatgactt agccgccacg ctggcggtca aagtgacaca ggccgggcat
    20041 caggcaacga ttgtttcgac agataaaggc tactgtcagt tactttcacc gacattacgt
    20101 attcgtgatt acttccagaa acgttggctg gatgcgccat ttatcgataa agaatttggc
    20161 gttcaaccgc agcagttgcc cgattactgg ggacttgcgg ggatcagcag ttcaaaggta
    20221 ccgggtgttg cgggaatcgg accaaaaagc gccacgcagc tgctggtcga gtttcagagt
    20281 ctggaaggga tatatgagaa tctggatgcg gttgccgaaa agtggcgcaa aaaattagaa
    20341 acccataaag agatggcgtt tctgtgccgc gatattgccc gcttacaaac cgatttgcat
    20401 atcgacggca atttacagca attgcggttg gtacggtaac ggcgagccgg atacgccgca
    20461 aacgtcgtat ccggcattat cacatcagcg catttaccag gcggtatggt aaagctctac
    20521 aatatcctca agcgttgctt cacgcgggtt gccaccggta caaacatcat ccagtgccgc
    20581 ctgcgccagt gccggaatgt cttccttgcg taccccaaca tcacgcaaat gtggcggaat
    20641 accgacatca cggttgagag caaacaccgc ttcaacagcg gcattacgcg cctcttccag
    20701 gctcatacct tccactttca cgcccataac gcgcgcgata tcgcggtact tctcaccggt
    20761 aaagtcagcg ttatagcgca tgacatgcgg tagcaggatg gcgtttgcaa caccgtgtgg
    20821 agtgttataa aacgcgccca gtggatgcgc cataccatgc accaacccta acccaacatt
    20881 cgagaagccc atacccgcaa catactgccc gagcgccatt tcttctccgg catccttatc
    20941 accagcaacc gatcctcgca gcgccccagc aatgatttca atcgctttaa tgtgcagtgc
    21001 atcggttagc gcccacgcgc cacgggtaat atacccctca atagcatgag tgagcgcatc
    21061 gacacccgtc gcagctttca gcgctggagg cataccatcc atcatgtcag cgtcaataaa
    21121 cgccacctgc gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt tttcttcgtc
    21181 agtgatcacg tagttaatgg tcacttctgc cgcagtgcct gctgtggtgg ggattgccag
    21241 aatcggtaca ctgggtttat tggtcgggga aagtccttcc aggctacgca catcggcaaa
    21301 ctccgggttg ttgctgataa tgccaatcgc tttacaagta tcctgtggag aaccaccacc
    21361 aatagcgatc aggtaatccg cgccgctatt ctggaataca ccgagccctt ctttgacgac
    21421 agtaattgtt gggttgggca ctacgccgtc gtaaatcgcc catgccagcc ctgcagcatc
    21481 catcttatcg gtcactttcg ccaccacgcc gcattgcacc agcgttttat cggtgacgat
    21541 cagcgccttc tgataaccac ggcgtttcac ctcatcggtt aaagccccaa cagcaccccg
    21601 accaaaccat gccgtttcgt tcagaatcat tctgttagcc atcatccttc tccttgttgc
    21661 tttacgaaat tactcttcaa ttcgtaaccc ataggttttg aatttctcca gcactacggc
    21721 aatctcttca tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg tcaggtaaag
    21781 ttgcgccagc acttcaactt catgcgccag ccataacgct ttttccagat tcacctcaca
    21841 agcgataagc ccatgatgtt gtaacaaagt tgccttacga tttttgagag cctgcgcaac
    21901 atgttcagaa agttcacgtg ttccaaaggt cgcataaggc gcgcaaggaa tagaattacc
    21961 gccagccgcc gcaatcatgt agtgaatagc ggggatcgat cggttaagaa tggaaactgc
    22021 cgtgcaatga acggcatgat tgtgaacaac cgcgttggca tccggtctgc tttgataggc
    22081 cgccatatgg aaacgccatt cgcttgaggg gagctttcct tcctcatgtt taccgttgcc
    22141 atcaataaag acaatatgcg actccgtcag tttttcatat ggaatgcctg taggcgtaat
    22201 cagcatccca tcctgataac gtacactgac gttccccgct gtcccctggt tcagtcccag
    22261 gcgggtcatt tccaggcaag tgtcaataat ctgacgagca agtttatttc gttccattag
    22321 ctacctctct ctgattcaaa acagggcaat aatgttgttc ctttcacact attgaattag
    22381 ccgtttaatt acccaccatc ttcttcctga ttaaccagaa agaaattcac aagcttatat
    22441 tttgtgacct ggttcaacta atcacagtaa ataact
//
LOCUS       DAZKGS010000066        22433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKGS010000066 DAZKGS010000000
VERSION     DAZKGS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1384)
                     /gene="djlB"
                     /locus_tag="ACHOQY_002536"
     CDS             complement(<1..1384)
                     /gene="djlB"
                     /locus_tag="ACHOQY_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP9678617.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIF"
     gene            complement(1381..2088)
                     /gene="ybeR"
                     /locus_tag="ACHOQY_002537"
     CDS             complement(1381..2088)
                     /gene="ybeR"
                     /locus_tag="ACHOQY_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9678618.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2252..3229
                     /gene="ybeQ"
                     /locus_tag="ACHOQY_002538"
     CDS             2252..3229
                     /gene="ybeQ"
                     /locus_tag="ACHOQY_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFP9678619.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3299..3781)
                     /gene="ybeL"
                     /locus_tag="ACHOQY_002539"
     CDS             complement(3299..3781)
                     /gene="ybeL"
                     /locus_tag="ACHOQY_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9678620.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4016..6598
                     /gene="leuS"
                     /locus_tag="ACHOQY_002540"
     CDS             4016..6598
                     /gene="leuS"
                     /locus_tag="ACHOQY_002540"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9678621.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6613..7194
                     /gene="lptE"
                     /locus_tag="ACHOQY_002541"
     CDS             6613..7194
                     /gene="lptE"
                     /locus_tag="ACHOQY_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9678622.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7194..8225
                     /gene="holA"
                     /locus_tag="ACHOQY_002542"
     CDS             7194..8225
                     /gene="holA"
                     /locus_tag="ACHOQY_002542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9678623.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8227..8868
                     /gene="nadD"
                     /locus_tag="ACHOQY_002543"
     CDS             8227..8868
                     /gene="nadD"
                     /locus_tag="ACHOQY_002543"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9678624.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8892..9503
                     /gene="cobC"
                     /locus_tag="ACHOQY_002544"
     CDS             8892..9503
                     /gene="cobC"
                     /locus_tag="ACHOQY_002544"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9678625.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9763..10080
                     /gene="rsfS"
                     /locus_tag="ACHOQY_002545"
     CDS             9763..10080
                     /gene="rsfS"
                     /locus_tag="ACHOQY_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9678626.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10084..10551
                     /gene="rlmH"
                     /locus_tag="ACHOQY_002546"
     CDS             10084..10551
                     /gene="rlmH"
                     /locus_tag="ACHOQY_002546"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9678627.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10582..12483
                     /gene="mrdA"
                     /locus_tag="ACHOQY_002547"
     CDS             10582..12483
                     /gene="mrdA"
                     /locus_tag="ACHOQY_002547"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9678628.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12486..13598
                     /gene="mrdB"
                     /locus_tag="ACHOQY_002548"
                     /gene_synonym="rodA"
     CDS             12486..13598
                     /gene="mrdB"
                     /locus_tag="ACHOQY_002548"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9678629.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13609..14697
                     /gene="rlpA"
                     /locus_tag="ACHOQY_002549"
     CDS             13609..14697
                     /gene="rlpA"
                     /locus_tag="ACHOQY_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9678630.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNQANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14837..16048
                     /gene="dacA"
                     /locus_tag="ACHOQY_002550"
     CDS             14837..16048
                     /gene="dacA"
                     /locus_tag="ACHOQY_002550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9678631.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16159..16422
                     /gene="ybeD"
                     /locus_tag="ACHOQY_002551"
     CDS             16159..16422
                     /gene="ybeD"
                     /locus_tag="ACHOQY_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9678632.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16523..17164
                     /gene="lipB"
                     /locus_tag="ACHOQY_002552"
     CDS             16523..17164
                     /gene="lipB"
                     /locus_tag="ACHOQY_002552"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9678633.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17423..18376
                     /gene="ybeF"
                     /locus_tag="ACHOQY_002553"
     CDS             17423..18376
                     /gene="ybeF"
                     /locus_tag="ACHOQY_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP9678634.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            18585..19550
                     /gene="lipA"
                     /locus_tag="ACHOQY_002554"
     CDS             18585..19550
                     /gene="lipA"
                     /locus_tag="ACHOQY_002554"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9678635.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(19650..19853)
                     /gene="tatE"
                     /locus_tag="ACHOQY_002555"
     CDS             complement(19650..19853)
                     /gene="tatE"
                     /locus_tag="ACHOQY_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9678636.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(19982..20770)
                     /locus_tag="ACHOQY_002556"
     CDS             complement(19982..20770)
                     /locus_tag="ACHOQY_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9678637.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            20863..21246
                     /gene="crcB"
                     /locus_tag="ACHOQY_002557"
     CDS             20863..21246
                     /gene="crcB"
                     /locus_tag="ACHOQY_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9678638.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21301..21510)
                     /gene="cspE"
                     /locus_tag="ACHOQY_002558"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21301..21510)
                     /gene="cspE"
                     /locus_tag="ACHOQY_002558"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9678639.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(21685..22245)
                     /gene="pagP"
                     /locus_tag="ACHOQY_002559"
     CDS             complement(21685..22245)
                     /gene="pagP"
                     /locus_tag="ACHOQY_002559"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9678640.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5735 a         5639 c         5562 g         5497 t
ORIGIN      
        1 taaaaatata gaaaataatt ttaatgatgc ccagacagcc tggcccacca tcgtcaacgg
       61 gcattggctg cggttgcggc tccggttcaa cagtttgctt ctcggtaaaa ttcgccgcct
      121 gaattaataa tcgacgaata attaatgctt ccagtggaca acgatcttct gcatcaaggc
      181 ggctcacaat cggcattaat gattcttcgg ttggttggtt cgcctgcact aattgtttgg
      241 caactaaata taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct
      301 tacgtccttt gtgcaaaacc atctcgacca gtgaacgccc cagagcagag agtttttcat
      361 cgctgagtat ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg atataaagta
      421 gcggttttcc ctgatcgtta accagttcac gagcttcaat catcatcacc actaacgggt
      481 aataatcgac ccgatgctga cggcaccatt gcaatattaa agcatccgcc tgggtggagg
      541 gatactcgcg ccagtagtca caaagttctg ccagcaagct ttcgccttct ccacaataga
      601 cgcgttgtgc gtattcataa taacgcgcat cttcattatc agattcagct tcccgccacg
      661 accgggcgta atccaggagt tcgggaattc ccaggcgacc tgcgctatac cagcggagta
      721 aatttctgtg cagttttgca tcatcgggga taatcactgc gcgatgttgc gctaaataca
      781 atgtcagcca ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatag
      841 tgtcattttg cacggcaacg ggcagatgca gaatggtctg gaagttaaaa acattcccct
      901 tcgcaatggc atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct
      961 cttgcaacca ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag
     1021 tgttcatggc gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat
     1081 aatttaattg ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg
     1141 caagtaacgc acgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt
     1201 gccaaacaga tttagcaggc gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa
     1261 gttgtttaaa accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag
     1321 cgcggcggat aatatcgacg tcagtcgttt cctctatttc gagaatcttc cagcaatttt
     1381 tcataggtcg ctcatatcct taagcgtttc cgggcatgca gaatagtaag ccaatggttg
     1441 ccaggacaag gtagggtaat acgcatcatc actggcaaat aattgataaa acatatcatt
     1501 gccggaatcg tcgtattctt tacgcgctaa gaattcgcgt aataactgca gatgatcatc
     1561 ttcgggagac tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt
     1621 acgataataa cgtagagccc gtagatgaat gcgcgactgg atccatagac tttcttcttc
     1681 gctcaaccag ttattaagta cgcccattcg gcttaaaaaa cccatccgga cataatccca
     1741 ggctttaatt cctccctcac cacagcattc ggcggtgctg gcgacaaatt gcgcataagc
     1801 ctgaccttgc tcatttagtt cagcaagata gtcacgccat tcacatggcg aataacgaaa
     1861 ccagcggtga taaaaccctg ccaaccgagc ggcatgaccg ttatcgatca ttcggtgaat
     1921 tgtgcgcagc aggtcatcac gggcatcaat tccccaggag gaagaaaggt caaaaaagca
     1981 tgtacctaaa tacattttgg gtgatgaatg cgaaggtaaa cagcattcat tgcgtatttc
     2041 catcggagtg gaaagtgcaa acaatatttt ttgcgactcc atgtccatat aatttatcct
     2101 taaatattca ttaaatgaat attagccgca ttgcgaggct cgtttattta tacttttctg
     2161 acgcgacata atacgcataa aataaggctt acgcaaaaat ggaacacata aaaatataat
     2221 atgttttatc tgcaaccata aggaaatgat aatgattttc acgtcaagtt gctgcgataa
     2281 tttatcaata gatgagatta tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat
     2341 tgttgggttt tattataatc gcgatagcgc aattgattct ccggacgacg aaaaagcctt
     2401 ttactggctg aagctggccg ctgagcaagg tcattgtgaa gcacagtatt ccttagggca
     2461 aaagtatacc gaggataaaa gccgtcataa agataatgag caagccatct tctggctgaa
     2521 aaaagctgcc ctacaaggcc atactttcgc ttccaacgcc cttggctgga cactggatcg
     2581 tggagaagcc cccaattata aagaagcggt tgtctggtat cagatagccg cggagagcgg
     2641 aatgtcttat gcgcaaaata atcttgggtg gatgtacaga aatggcaacg gagtcgcaaa
     2701 agactatgcg ctggcatttt tctggtacaa acaagctgca ttacaaggcc atagtgacgc
     2761 gcaaaacaat ctggccgatc tttatgaaga cggaaaaggc gttgctcaaa acaagacact
     2821 cgccgcattc tggtatttga aaagcgcaca gcagggtaat cggcacgccc agtttcaaat
     2881 tgcatgggat tataacgctg gcgaaggggt ggaccaggac tataagcaag cgatgtactg
     2941 gtatctgaag gctgccgctc aggaaagcgt cggcgcttac gtcaacatcg gttatatgta
     3001 taaacacgga caaggcgttg agaaagatta tcaggctgcc tttgaatggt ttacgaaagc
     3061 cgctgaatgc aatgacgcca ctgcctggta taacctggcc attatgtatc actacggaga
     3121 aggaagacct gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg
     3181 aaccagagat gtcagtcagg aaattcgtga gactgaagat ttactgtaga gataaaaaca
     3241 agggccgggg aatttcattc ggcccatatt ctctttattg ctgtgcgaaa tcagactatt
     3301 acggctcaaa cgggcgtctc tggaactggt catgaccaca tttcgggcat agcgtcagca
     3361 cttccggtgt gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc
     3421 cgaccacttc tccgctgtga taaaccccat gatgattgag gtcctggaaa acttcgcgcc
     3481 attcaagctg cgttttatcg gtgatatctg ccagctcctg ccataagctt tctttaatca
     3541 cccgcataaa gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact
     3601 cttccaggtc acgtcttaca gctcgcgtca gctcatcgac ctcggttcgc gttaactccc
     3661 ctgtttttat tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat
     3721 tgcgcaggcg ttcgctcagt gacgcgacca gttcacggta atattgagca accttgttca
     3781 tcgtttcatc tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt
     3841 tgctaatacg cccacaaact ctgtaaatta atgtatgaat aatccgggca tcatttcaac
     3901 gtcgagtgcg cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa
     3961 aaaccacctc aacgctacat ttgtagccgt attgaaaaca ggaccactgg ctgccatgca
     4021 agagcaatac cgcccggaag agatagaatc caaagtacag cttcattggg atgagaagcg
     4081 cacatttgaa gtaaccgaag acgagagcaa agagaagtat tactgcctgt ctatgcttcc
     4141 ctatccttct ggtcgactac acatgggcca cgtacgtaac tacaccatcg gtgacgtgat
     4201 cgcccgctac cagcgtatgc tgggcaaaaa cgtcctgcag ccgatcggct gggacgcgtt
     4261 tggtctgcct gcggaaggcg cggcggtgaa aaacaacacc gctccggcac cgtggacgta
     4321 cgacaacatc gcgtatatga aaaaccagct caaaatgctg ggctttggtt atgactggag
     4381 ccgcgagctg gcaacctgta cgccggaata ctaccgttgg gaacagaaat tcttcaccga
     4441 gctgtataaa aaaggcctgg tatataagaa gacttctgcg gtcaactggt gcccgaacga
     4501 ccagaccgta ctggcgaacg aacaagttat cgacggctgc tgctggcgct gcgataccaa
     4561 agttgaacgt aaagagatcc cgcagtggtt tatcaaaatc actgcttacg ctgacgagct
     4621 gctcaacgat ctggataaac tggatcactg gccagacacc gttaaaacca tgcagcgtaa
     4681 ctggatcggt cgttccgaag gcgtggagat caccttcaac gttaacgact atgacaacac
     4741 gctgaccgtt tacactaccc gcccggacac ctttatgggt tgtacctacc tggcggtagc
     4801 tgcgggtcat ccgctggcgc agaaagcggc ggaaaataat cctgaactgg cggcctttat
     4861 tgacgaatgc cgtaacacca aagttgccga agctgaaatg gcgacgatgg agaaaaaagg
     4921 cgtcgatact ggctttaaag ctgttcaccc attaacgggc gaagaaattc ccgtttgggc
     4981 agcaaacttc gtattgatgg agtacggcac gggcgcagtt atggcggttc cgggtcacga
     5041 ccagcgcgac tacgagtttg cctctaaata cggcctgaac atcaaaccag ttatcctggc
     5101 tgctgacggc tctgagccag atctttctca gcaagccctg actgaaaaag gcgtgctgtt
     5161 caactctggc gaattcaacg gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa
     5221 actgactgcg atgggcgttg gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt
     5281 ttctcgtcag cgttactggg gcgcgccgat tccgatggtg acgctggaag acggtaccgt
     5341 aatgccgacc ccggacgacc agctgccggt gatcctgccg gaagatgtgg tgatggacgg
     5401 cattaccagc ccgattaaag cagatccgga gtgggcaaaa actaccgtta acggtatgcc
     5461 agcgctgcgt gaaaccgaca ctttcgacac ctttatggag tcctcctggt actatgcgcg
     5521 ctacacttgc ccagagtaca aagaaggtat gctggattcc gaagcggcta actactggct
     5581 gccggtggat atctacattg gtggtatcga acacgccatt atgcacctgc tctacttccg
     5641 cttcttccac aaactgatgc gtgatgcagg catggtgaac tctgacgaac cagcgaaaca
     5701 gttgctgtgt cagggtatgg tgctggcaga tgccttctac tatgttggcg aaaacggcga
     5761 acgtaactgg gtttccccgg ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt
     5821 gaaagcgaaa gatgcggcag gccatgaact ggtttatacc ggcatgagca aaatgtccaa
     5881 gtcgaagaac aacggtatcg acccgcaggt gatggttgaa cgttacggcg cggacaccgt
     5941 tcgtctgttt atgatgtttg cttctccggc tgatatgact ctcgaatggc aggaatccgg
     6001 tgtggaaggg gctaaccgct tcctgaaacg tgtctggaaa ctggtttacg agcacacagc
     6061 aaaaggtgat gttgcggcac tgaacgttga tgcgctgact gaagatcaga aagcgctgcg
     6121 tcgcgatgtg cataaaacga tcgctaaagt gaccgatgat atcggccgtc gtcagacctt
     6181 caacaccgca attgcggcga ttatggagct gatgaacaaa ctggcgaaag caccaaccga
     6241 tggcgagcag gatcgcgctc tgatgcagga agcgctgctg gccgttgtcc gtatgcttaa
     6301 cccgttcacc ccgcacatct gcttcacgct gtggcaggaa ctgaaaggcg aaggcgatat
     6361 cgacaacgcg ccgtggccgg ttgctgacga aaaagcgatg gtggaagact ccacgctggt
     6421 cgtggtgcag gttaacggta aagtccgtgc caaaatcacc gttccggtgg acgcaacgga
     6481 agaacaggtt cgcgaacgtg ctggccagga acatctggta gcaaaatatc ttgatggcgt
     6541 taccgtgcgt aaagtgattt acgtaccagg taaactcctc aatctggtcg ttggctaagc
     6601 gcgggaggaa gcgtgcgata tctggcaaca ttgttgttat ctctggctgt gttaatcacc
     6661 gccgggtgtg gctggcatct gcgtgatacc acgcaggttc cttccactat gaaggtcatg
     6721 atcctggact caggcgatcc gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt
     6781 ctgaatggtg tcgagttgct tgataaagaa accacgcgta aggacgttcc atccctgcgt
     6841 ttgggtaaag tgagcatcgc gaaagatacc gcatcggtat tccgtaacgg tcaaacagca
     6901 gagtatcaga tgatcatgac ggtgaatgcg accgtgttga tccccggccg tgatatctac
     6961 ccgattagtg ccaaagtctt ccgttcgttc tttgataacc cgcaaatggc gttagcgaaa
     7021 gataacgaac aagacatgat cgtaaaagag atgtacgacc gtgctgccga acagctgatt
     7081 cgtaagctgc caagcatccg tgctgcggat attcgttccg acgaagaaca gacgtcgacc
     7141 acaacggata ctccggcaac gcctgcacgc gtctccacca cgctgggtaa ctgatgattc
     7201 ggttgtaccc ggaacaactc cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt
     7261 tacttggtaa cgatcctctg ttattgcagg aaagccagga cgctgttcgt caggtagctg
     7321 cggcacaagg attcgaagaa caccacactt tttccattga tcccaacact gactggaatg
     7381 cgatcttttc gttatgccag gctatgagtc tgtttgccag tcgacaaacg ctattgctgt
     7441 tgttaccaga aaacggaccg aatgcggcga tcaatgaaca acttctcaca ctcaccggac
     7501 ttctgcatga cgacctgctg ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa
     7561 atgccgcctg gtttactgct cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg
     7621 agcaggctca gcttccccgc tgggttgctg cgcgcgcaaa acagctcaac ttagaactgg
     7681 atgacgctgc aaatcaggtg ctctgctact gttatgaagg taacctgctg gcgctggctc
     7741 aggcactgga gcgtttatcg ctgctctggc cagacggcaa attgacatta ccgcgcgttg
     7801 aacaggcggt gaatgatgcc gcgcatttca ccccttttca ttgggttgat gctttgttga
     7861 tgggaaaaag taagcgcgcg ttgcatattc ttcagcaact gcgtctggaa ggcagcgaac
     7921 cggttatttt gttgcgcaca ttacaacgtg aactgttgtt actggttaac ctgaaacgcc
     7981 agtctgccca tacgccactg cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc
     8041 ggggcatgat gggcgaggcg ttaaatcgct taagtcagac gcagttacgt caggccgtgc
     8101 aactcctgac acgaacggaa ctcaccctca aacaagatta cggtcagtca gtgtgggcag
     8161 agctggaagg gttatctctt ctgttgtgcc ataaacccct ggcggacgta tttatcgacg
     8221 gttgatatga aatctttaca ggctctgttt ggcggcacct ttgatccggt gcactatggt
     8281 catcttaaac ccgtggaaac gctggcgaat ttgattggtc tgacgcgggt cacaatcatc
     8341 cctaataatg ttcctccgca tcgtccccag ccggaagcga acagcgtgca gcgtaaacac
     8401 atgcttgaac tggcgattgc cgacaagcca ttatttactc ttgatgaacg cgagctaaag
     8461 cgcaatgccc cctcttacac tgcgcaaaca ctgaaagagt ggcggcagga acaagggccg
     8521 gacgtgccgc tggcgtttat tattggtcag gattcattgc tgacctttcc gacctggtac
     8581 gaatacgaaa cgatactcga caatgcacat ttgatcgtct gtcggcgtcc aggttaccca
     8641 cttgaaatgg cgcaaccgca ataccagcaa tggctggaag atcatttgac acataacccg
     8701 gaagatcttc accttcagcc tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac
     8761 atctcggcga ccatcatccg cgaacgtttg caaaacggtg aatcgtgtga ggatttattg
     8821 ccggaaccgg tactgactta cattaaccaa caaggcttgt atcgctgata cctgtcgtta
     8881 cggagggcat gatgcgactg tggttaattc gtcatggtga aacgcaagcg aatatcgatg
     8941 gtctttacag cggtcatgcg cccacccccc tgaccgcgcg cggtattgag caagcgcaaa
     9001 atctgcatac actgctagat gatgtttcct ttgacctggt tttatgcagt gaactggaac
     9061 gggcacagca taccgcgcga cgggttctca gtgaccgcca gctccccgtg caaatcatcc
     9121 ctgaactcaa cgaaatgttt tttggcgact gggagatgcg acatcatcgc gacctcatgc
     9181 aagaagatgc cgaaaactat agcgcgtggt gcaatgactg gcagcatgct atccccacga
     9241 acggtgaagg atttcaggca ttttcgcaac gtgtggaaag atttatcgca aggcttagtg
     9301 aatatcagca ctatcagaat attttaatcg tcagccatca gggcgtactg agtctgttaa
     9361 ttgctcgtct aattggcatg cctgccgaat ctatgtggca ttttcgcgtt gaccaggggt
     9421 gttggagcgc cattgatatc aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg
     9481 ccatcggggt cgagaatgca tgacttttct gtttttttac gggtaagccg caacgaccat
     9541 tgacagcccc gggcaggctg atattctccg cagccagact ttttccgcca gacacgactt
     9601 tgtagaaatt gttttacaaa aatggcgatg caatctgcgg cgcggggtgg gatgatagcc
     9661 cactttcgaa agccgattcg gcgacaattg tcccgaaatc gcctctggtt caggtatact
     9721 gacagaccat ttttatctat ttgattcacc cagggggaaa acttgcaggg taaagcactc
     9781 caggattttg ttatcgacaa aattgatgac ctcaaaggtc aggacatcat cgccttagac
     9841 gttcagggca aatccagcat caccgactgc atgatcatct gtacgggtac gtccagccgt
     9901 catgttatgt ccattgctga ccacgttgtg caggagtctc gcgcagcggg cctgttaccg
     9961 ctcggcgtag aaggtgaaaa cagcgccgac tggattgtcg tggatttggg cgatgtgatt
    10021 gtccatgtca tgcaggaaga gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa
    10081 tgcgtgaagc tgcaacttgt cgccgtggga acgaaaatgc cggactgggt acaaaccggt
    10141 tttaccgagt acctgcgtcg ttttccgaaa gatatgccct tcgagctgat tgaaattccg
    10201 gccggaaaac gcggcaagaa tgcggacatc aagcgcatac tcgacaaaga gggtgagcag
    10261 atgttggcgg ccgcaggcaa aaaccgcatt gtcaccctcg atattccagg caagccctgg
    10321 gatacgccgc agttagccgc tgagctggaa cgctggaagc tggatggtcg cgacgtcagt
    10381 ctactgattg gcgggcctga agggttgtcg cctgcctgta aagcggcggc ggagcagagc
    10441 tggtcgctgt cggcgcttac cctcccccat ccgctggttc gcgtgctggt cgcagagagt
    10501 ctgtaccggg cgtggagcat caccaccaac catccttatc accgtgagtg ataaaggagc
    10561 tttgagtaga aaacgcagcg gatgaaacta cagaactctt ttcgcgacta tacggcagag
    10621 tccgcgctgt ttgtgcgccg ggcgctggtc gcctttttgg ggattttgct gctgaccggc
    10681 gtgcttatcg ccaacctgta taatctgcaa attgttcgct ttaccgatta ccagacccgc
    10741 tctaatgaaa accgcattaa gctggtgcct atcgcgccca gccgcggcat tatctacgac
    10801 cgtaacggta tccctctggc actcaaccgc actatctacc agatagaaat gatgccggag
    10861 aaagtcgata acgtgcagca aacgctggac gctttgcgca gcgtggttga tctgaccgat
    10921 gacgatattg ctgcattccg aaaagagcgc gcacgttcac accgtttcac ctctattccc
    10981 gtgaaaacta acctgaccga agtacaagta gctcgctttg ccgtcaatca gtaccgtttt
    11041 ccgggtgtcg aagttaaagg ctataaacgt cgttactatc cttacggttc ggcgttgacc
    11101 cacgtcatcg gctatgtgtc gaaaatcaac gataaagacg tcgaacgcct gaataatgac
    11161 ggcaaactgg ccaactatgc ggcaacgcat gatatcggta agctgggcat tgagcgttac
    11221 tatgaagacg tgctgcacgg tcagaccggt tatgaagagg ttgaagttaa caaccgtggg
    11281 cgtgttatcc gccagttaaa agaagtacca ccgcaagccg gacacgatat ttacctgacg
    11341 ctggatctca aactccagca atatattgaa acgctgctgg cgggtagccg cgcagctgtg
    11401 gtagtcaccg atccgcgtac aggtggggtg ctggcactgg tttccacgcc tagttatgac
    11461 ccaaacctgt ttgttgacgg tatctccagc aaagattatt ccgccttgct gaacgacccg
    11521 aatacaccgc tggtgaaccg cgccacacag ggggtttatc ctcctgcgtc tacagttaaa
    11581 ccctatgtgg cggtttcggc attaagcgcc ggggtgatca cgcgcaatac gacgctgttt
    11641 gacccaggct ggtggcaact accagggtcg gaaaaacgtt atcgtgactg gaaaaaatgg
    11701 ggccacgggc gtctgaatgt cacaagatcg ctggaagaat ctgcggatac cttcttctat
    11761 caggtggcct acgatatggg gatcgatcgc ctctccgaat ggatgggtaa attcggttat
    11821 ggtcattaca ccggtatcga cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa
    11881 tggaaacaga aacgctttaa aaaaccgtgg tatcagggtg acaccattcc ggttggtatc
    11941 ggccagggtt actggacagc gaccccaatc cagatgagta aggcactgat gatcctgatt
    12001 aatgacggta tcgtgaaggt tccgcatttg ctgatgagca ccgccgaaga cggcaaacag
    12061 gtgccatggg tacaaccgca tgaaccgccc gtcggcgata ttcattccgg ttactgggag
    12121 ctggcgaaag acggtatgta cggtgttgct aaccgcccta acggtacggc gcataaatac
    12181 tttgccagcg caccgtacaa aattgcggcg aaatctggta ccgctcaggt cttcggtctg
    12241 aaagcgaacg aaacctataa tgcgcacaaa attgccgagc gtttacgtga ccacaaactg
    12301 atgaccgcct ttgcgccata caacaatccg caagtggctg tcgccatgat tctggagaac
    12361 ggcggcgcgg gtccggcggt tggtacgctg atgcgccaga tcctcgacca cattatgctg
    12421 ggtgataaca ataccgatct gcctgcggaa aatcctgcgg ttgccgcagc ggaggaccat
    12481 taatcatgac ggataatccg aataaaaaaa cattctggga taaagtccat ctcgatccca
    12541 caatgctgct gatcttactg gcattgctgg tttacagcgc cctggttatc tggagcgcca
    12601 gcggtcagga tattggcatg atggagcgta aaataggcca aatcgcgatg ggtctggtca
    12661 tcatggtggt gatggcgcaa attcctccac gcgtttatga aggctgggcc ccctatctct
    12721 atatcatctg tattattttg ctggtggcgg tagatgcttt cggtgccatc tctaaaggcg
    12781 ctcaacgctg gctggacctc ggtattgttc gttttcagcc gtcggaaatt gccaaaatag
    12841 ccgtaccact gatggttgcg cgctttatca accgcgacgt ttgcccgcca tcgttgaaga
    12901 acactggcat cgcgctggtg ctgatattta tgcccacgct gctggtggct gcacagcctg
    12961 acctgggaac atcaatcctc gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc
    13021 ttagctggcg tctgattggc gtcgcagtag tgctggtggc ggcgttcatt cctattctgt
    13081 ggttcttcct gatgcatgat taccagcgcc agcgcgtaat gatgctcctg gacccggaat
    13141 cagacccact cggcgcgggc tatcacatta ttcagtctaa aattgctatt ggctccggcg
    13201 gattacgcgg caaaggctgg ctgcatggca ctcagtcaca acttgaattt ctacccgaac
    13261 gccataccga ctttatcttc gcggtactgg cggaagagct gggattagtg ggcattctga
    13321 ttctgctcgc tctctacatt ctgctgatca tgcgcgggct gtggatagcc gccagagcgc
    13381 aaaccacctt tggtcgcgtc atggctggcg gcttaatgct gatattattc gtttatgtct
    13441 tcgtaaatat tggtatggta agcggtattc tgccggttgt aggggttccg ctcccactgg
    13501 tcagttatgg aggatcagcg ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa
    13561 tccacaccca caggaaaatg ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag
    13621 tggctcggga tctgcatcgc ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag
    13681 caacagacgg taagtgtacc gcagcctgcg gtatgtaacg gccccatagt tgaaattagc
    13741 ggggcggacc cgcgtttcga accactgaac gcgacggcaa atcaggatta ccagcgcgac
    13801 ggtaaaagct acaaaatcgt gcaggatccg tctcgattta gccaggcggg actggcggca
    13861 atctatgatg ccgaaccagg cagtaacctg acggcctctg gcgaagcttt cgatccgaca
    13921 cagttgacgg cggcccatcc aacgcttccg atccccagct acgccagaat cactaaccag
    13981 gctaacgggc gaatgatcgt ggtgcgcatt aatgatcgcg gcccctacgg caacgaccgc
    14041 gtaatttcgc tttctcgcgc agcagctgac cgtcttaaca cgtcaaacaa caccaaagtt
    14101 cgtatcgatc cgattattgt cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt
    14161 accacagtcg ccaaacagac ttacgccctg cctgcacctc ccgatttaag cggtggcgcg
    14221 ggggcaagtt cagtgtctgg cccgcagggt gacattcttc cggtcagtaa ctcgacgcta
    14281 aaaagcgaag atccgaccgg cgcgccggta accagtagcg gtttcctcgg cgctccaacg
    14341 accttagcgc ctggtgtact ggaaagcagc gaaccgacac ctgcaccaca gcccgttgtt
    14401 acagctccgt cgacaacgcc tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg
    14461 caaagcgcca gcggcaactt tatggtgcaa gtcggggccg ttagcgatca ggctcgtgcg
    14521 caacagtacc aacagcaact gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat
    14581 ggcgcggtct ggcgtatcca gcttggccca ttcgccagta aagccgaagc cagtaccttg
    14641 cagcaacgtt tgcaaaccga agcccaatta cagtcattta ttaccaccgc gcagtagcgt
    14701 aaagcaggca tctgatgtgt caatttctgt aaatgacgtt aacaaagtca cgcggaaagt
    14761 cagatgcctg ccggtagtgc atttgctata gtagggcact ttttttaatt ccatcacgga
    14821 tgtcgtagtt cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct
    14881 caccacggct ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa
    14941 aactatgatc ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa
    15001 ctccggcaaa gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ctagcctgac
    15061 caaaatgatg accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac
    15121 tgatttagtc actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc
    15181 ttcgctgatg ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat
    15241 taacctgcaa tcgggtaacg atgcttgcgt cgccatggcc gattttgccg ctggtagcca
    15301 ggacgctttt gttggcttga tgaacagcta cgttaacgcc ctgggcctga aaaacaccca
    15361 cttccagacg gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc
    15421 gctgatcggc caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa
    15481 agaatttacg tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag
    15541 cctgaatgtc gacggcatca aaaccggaca cactgacaaa gcaggttaca accttgttgc
    15601 ttctgcgact gaaggccaga tgcgcttgat ttctgcggtg atgggcggac gtacttttaa
    15661 aggccgtgaa gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt
    15721 taacccactg aaagtaggta aagagttcgc ctctgaaccg gtttggtttg gtgattctga
    15781 tcgcgcttcg ttaggggttg ataaagacgt gtacctgacc attccacgtg gtcgcatgaa
    15841 agatctgaaa gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa
    15901 tcaggtcgtc ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct
    15961 ggttgtgttg caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa
    16021 attaatgttc catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc
    16081 ccatatacta agcatcagta aaaaaactcc cgccttctgg cgggagttgc tatttaatta
    16141 cgttacgccg gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct
    16201 tttacttaca aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg
    16261 gtacagcgcc atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac
    16321 taccactcgg tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat
    16381 gaagaactgg gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc
    16441 ggctcctggt cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact
    16501 cattctccac ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct
    16561 tcagccttac gagccaatct cccaggctat gcatgaattc accgataccc gcgatgatag
    16621 tacccttgat gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg
    16681 aaaagcggag cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg
    16741 gcaggtgact tatcacgggc cgggacaaca ggtgatgtat gtgttgctta acctgaaacg
    16801 ccgtaaactc ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct
    16861 ggctgaactg ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga
    16921 aaagaaaatt tgctcactgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc
    16981 attaaacgtc aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg
    17041 aatggaaatg gctaaaatat cacaatggaa acccgaagcg acgactaata atattgctcc
    17101 acgtttactg gaaaatattt tagcgctact aaacaatccg gacttcgaat atattaccgc
    17161 ttaattccat acatcaatgg cccaatttac actgggtcat tactcataat acctgctaat
    17221 gctttacatc tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcggtgccac
    17281 gccttgctcc gcaactcacc ccattgatta acctattgaa atagttgatt atggtgagca
    17341 aaagcgttcc gttccaatta gacataagat tattgtaatt ttttaaatat tatttttcca
    17401 tgaataaaat tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag
    17461 aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct
    17521 tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct
    17581 taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga
    17641 cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca
    17701 tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta
    17761 tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc
    17821 tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat
    17881 cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt
    17941 ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg
    18001 ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc
    18061 gcatgtactt ctgttaccgg aagagcaaaa tttcagcggt ctgcggcaga gagttcaaga
    18121 aatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact
    18181 ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagccg
    18241 ctgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc
    18301 tatccactac aacaaattca gcctgcgtga tcccatcctg cacggagtaa tagatgttat
    18361 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
    18421 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
    18481 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
    18541 ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg
    18601 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
    18661 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
    18721 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
    18781 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa
    18841 cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg
    18901 gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata
    18961 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
    19021 cccagcactt tgcggattgc attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
    19081 aaactctggt gccggatttc cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa
    19141 cgccaccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cgtcaggtac
    19201 ggcctggtgc agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc
    19261 cggaaatccc gaccaagtct ggtctgatgg tgggactggg tgaaaccaat gaagaaatta
    19321 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt
    19381 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
    19441 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
    19501 gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa tcctgtaata
    19561 atacttacat ctgtctgata aaaaacccgc tttttgaagc gggttttttg tatcgaacaa
    19621 gatattgagg gagcgtcctg ctcgccacgt cactctttat gagagagctt ttcagcctga
    19681 agatcaacgt ctgcgccttt tttcgccgca gcatcgtcat cattcatcgc cttcttgaac
    19741 cctttaatgg ccgctccaag gtctccgccc agcgtacgta acttcttagt cccaaacagc
    19801 agaacgacca gcgccgcaac tactagcagt ttggtaatac taatctcacc catagatacc
    19861 ttcttgacat ataaccggca acagaacgcg ccttaaaaac cagagaatat taacggtcat
    19921 tcgacgcgcg cacacaatag caatctgtaa caaggtgaat caagcgccga gtaaaaaaac
    19981 atcataataa ttgcggcggc gcaaagcgac ggttgtttaa gacgggcagt tgcgcgcgca
    20041 cctgacgcac acgttcgggc gtcacttccg ccataatgag tgcaggcatt tctgacgctg
    20101 ccgcaatggt gacgccaaag ggatctataa tccggctttg accgatattt ttgttcccgc
    20161 actcccccgc cgccaccata taacaggtgg tatccagcgc acgagcggca agcaacgttg
    20221 accaatgatg ctctttgagc ggcccgcgaa cccaggcggc aggaagtacc aggatttcag
    20281 ctccctgtaa tgcctgtgcc agcgccagct ctggaaagcg taagtcataa caggtcatca
    20341 gaccgacctt catcccctcc acctcgagta acggagcgat ttcattacca gcatcaacac
    20401 ggcgtgattc ctgaatggca aatgcatcat agagatgcaa tttggcataa cgggcgacga
    20461 tgtttcctgc ctgtagcgcc accagcatat tccatgcgcg ccccggcgtt gaaggaacat
    20521 gaatcgtcag aattgtcgtc atcatgttac gtttactttc tcgccgtaaa cgtccgagga
    20581 attcgccttc cagcagctgt gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg
    20641 ccagcaatgc ttccggcagg acgaacagcg atacgtcgtt ttccgccgcc tgcgccatca
    20701 acgaggcaca aatctcagcg ttcttttccc acacagatgt aacagcaaac tgtcctgcgg
    20761 caaccagcat tgccacctcc tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg
    20821 ttacacttat cactcataca aatcaaatag caggattttg cagtgttaca acttctttta
    20881 gcagttttta ttggcggtgg tacgggaagc gtggcgagat ggctgttaag tatgcgattt
    20941 aacccgctgc atcaggcgat tccgttgggg acgctggcag caaacctgat tggggcattc
    21001 atcataggaa tgggattcgc ctggttcagc aggatgacga acattgatcc agtgtggaaa
    21061 gtattaatca ccaccggatt ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa
    21121 gtggtgtttt tgttacaaga gggccgcttt ggctgggcgt tactgaacgt tttcgtcaac
    21181 cttctggggt cttttgccat gacagcactg gctttctggc tgttttcggc ctcaaccgca
    21241 cactaaagac caaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc
    21301 ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc
    21361 tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac
    21421 gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa
    21481 ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac
    21541 aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtccagt gcaactttac
    21601 taaaaagtga taaaagtcgc gttatttttg atgttactta ttttagctat tgattttaaa
    21661 gaagttacta aaacttcatt tgtctcaaaa ctgaaagcgc atccaggcaa agtacacatt
    21721 gccattgttg taggtacccg gaatgtaggt catctgaaaa gtcactgggc cataacccac
    21781 ggaggccaat ggcagtagaa ccgggagagg gatgtaattc cagttatcgc gtgccgttac
    21841 gccagcggtg aatcccagac ctaaatgaaa attttcatcc gccagcggtc gccaggtact
    21901 ttcccatccg tatccggcaa tcggttccca tttgttccac gagtccttaa atgccatggc
    21961 atacaggcca tgccagtttc ctttttcatc ccaacgcgac aggccaaaac cgccacccca
    22021 cggtcgctcg ttatagcgat cggttttttc tttgtcgtaa gcgaaacgtg catgccaggt
    22081 gatggcagga atatataaat cataatgttc aggctgttgc caggtttgtg caatattttc
    22141 tctaaacgtt gtcatccact catctgcgtt agcaaaaact ttaccaacgc tgattaactg
    22201 aataaaaaca aaggaaaaga tagcgacata tttactcacg ttcatttgtg accataaaac
    22261 atttatcaaa aatctactac tagcatagca aagctacaat taacataacc ttaatagacc
    22321 caacataaag aataatctga atagctgctc tatttatttc cgcttgttca agttaaatat
    22381 ttccgcgcat cgattaaaga tgatgcgtcg ttttctgcga tgggaatagt caa
//
LOCUS       DAZKGS010000067        21938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKGS010000067 DAZKGS010000000
VERSION     DAZKGS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..118
                     /locus_tag="ACHOQY_002560"
     tRNA            42..118
                     /locus_tag="ACHOQY_002560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:76..78,aa:Pro,seq:tgg)
     gene            265..1500
                     /gene="aslB"
                     /locus_tag="ACHOQY_002561"
     CDS             265..1500
                     /gene="aslB"
                     /locus_tag="ACHOQY_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9678641.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1608..1745)
                     /locus_tag="ACHOQY_002562"
                     /pseudo
     CDS             complement(1608..1745)
                     /locus_tag="ACHOQY_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(2424..3620)
                     /gene="hemY"
                     /locus_tag="ACHOQY_002563"
     CDS             complement(2424..3620)
                     /gene="hemY"
                     /locus_tag="ACHOQY_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9678642.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(3623..4840)
                     /gene="hemX"
                     /locus_tag="ACHOQY_002564"
     CDS             complement(3623..4840)
                     /gene="hemX"
                     /locus_tag="ACHOQY_002564"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9678643.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(4862..5602)
                     /gene="hemD"
                     /locus_tag="ACHOQY_002565"
     CDS             complement(4862..5602)
                     /gene="hemD"
                     /locus_tag="ACHOQY_002565"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9678644.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(5599..6540)
                     /gene="hemC"
                     /locus_tag="ACHOQY_002566"
     CDS             complement(5599..6540)
                     /gene="hemC"
                     /locus_tag="ACHOQY_002566"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9678645.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            6927..9473
                     /gene="cyaA"
                     /locus_tag="ACHOQY_002567"
     CDS             6927..9473
                     /gene="cyaA"
                     /locus_tag="ACHOQY_002567"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9678646.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(9513..9833)
                     /gene="cyaY"
                     /locus_tag="ACHOQY_002568"
     CDS             complement(9513..9833)
                     /gene="cyaY"
                     /locus_tag="ACHOQY_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9678647.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9881..11206)
                     /locus_tag="ACHOQY_002569"
     CDS             complement(9881..11206)
                     /locus_tag="ACHOQY_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9678648.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(11203..12711)
                     /locus_tag="ACHOQY_002570"
     CDS             complement(11203..12711)
                     /locus_tag="ACHOQY_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9678649.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(13049..13603)
                     /locus_tag="ACHOQY_002571"
     CDS             complement(13049..13603)
                     /locus_tag="ACHOQY_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP9678650.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            13953..14156
                     /gene="yifL"
                     /locus_tag="ACHOQY_002572"
     CDS             13953..14156
                     /gene="yifL"
                     /locus_tag="ACHOQY_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9678651.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            14193..15017
                     /gene="dapF"
                     /locus_tag="ACHOQY_002573"
     CDS             14193..15017
                     /gene="dapF"
                     /locus_tag="ACHOQY_002573"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9678652.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            15014..15721
                     /gene="yigA"
                     /locus_tag="ACHOQY_002574"
     CDS             15014..15721
                     /gene="yigA"
                     /locus_tag="ACHOQY_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFP9678653.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            15718..16614
                     /gene="xerC"
                     /locus_tag="ACHOQY_002575"
     CDS             15718..16614
                     /gene="xerC"
                     /locus_tag="ACHOQY_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9678654.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            16614..17330
                     /gene="yigB"
                     /locus_tag="ACHOQY_002576"
     CDS             16614..17330
                     /gene="yigB"
                     /locus_tag="ACHOQY_002576"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9678655.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            17414..19576
                     /gene="uvrD"
                     /locus_tag="ACHOQY_002577"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             17414..19576
                     /gene="uvrD"
                     /locus_tag="ACHOQY_002577"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9678656.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(19723..20487)
                     /gene="yigE"
                     /locus_tag="ACHOQY_002578"
     CDS             complement(19723..20487)
                     /gene="yigE"
                     /locus_tag="ACHOQY_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP9678657.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            20649..20705
                     /gene="ysgD"
                     /locus_tag="ACHOQY_002579"
     CDS             20649..20705
                     /gene="ysgD"
                     /locus_tag="ACHOQY_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9678658.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            20857..21807
                     /gene="corA"
                     /locus_tag="ACHOQY_002580"
     CDS             20857..21807
                     /gene="corA"
                     /locus_tag="ACHOQY_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9678659.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(21849..>21938)
                     /locus_tag="ACHOQY_002581"
     CDS             complement(21849..>21938)
                     /locus_tag="ACHOQY_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678660.1"
                     /translation="INDFTEYQLTQENKIERVFNPHDFKKDNF"
BASE COUNT         5134 a         5612 c         5732 g         5460 t
ORIGIN      
        1 gactttaaag aattgaacta aaaattcaaa aagtagtatt tcggcgagta gcgcagcttg
       61 gtagcgcaac tggtttggga ccagtgggtc ggaggttcga atcctctctc gccgaccaat
      121 tttgaacccc gcttcggcgg ggttttttgt tttctgtgca tttcgtcacc ctcccttcgc
      181 aataaacgcc cgtaataact cattgcccca cggtatgatt tcgcccttaa cgtattgaag
      241 gatgacttca ggcaaggagt gaccatgctg caacaggttc caacgcgtgc ttttcatgtg
      301 atggcgaaac cgagtggttc cgattgtaat ctgaactgtg actactgttt ttatctcgaa
      361 aaacaatccc tttaccgcga aaagccagtc acgcatatgg acgatgacac gctggaagcg
      421 tatgtccgtc actatatcgc tgccagcgaa ccgcaaaacg aagtggcttt tacctggcag
      481 ggcggcgaac caacgttact cgggctggat ttttaccgcc gtgccgtaaa gttacaggcg
      541 aaatacggtg ctggcaggaa gataagtaac agcttccaga ctaacggcgt gctgctcgat
      601 gataaatggt gtgcatttct ggcagaaaat cattttcttg ttgggttatc gctggacggt
      661 ccggctgaga tccacaatca atatcgcgtg accaaaggtg gcagaccaac gcataagctg
      721 gtgatgcgtg ccctgacgct gctgcaaaaa catcatgtcg actataacgt gctggtctgc
      781 gtcaaccgca ccagcgcgca gcaaccgttg caggtttatg attttttgtg cgatgcggga
      841 gtcgaattca tccagtttat tccggtggtc gagcgcctgg ctgatgaaac agctgccagc
      901 gatggactga aactacatgc gcctggtgat attcaggggg aactgacgga atggtcggtg
      961 cgccccgatg aatttggtga atttctggtg gcgatattcg accactggat caaacgcgac
     1021 gtcggcaaga ttttcgtgat gaatatcgaa tgggcgtttg ccaattttgt cggtgcgccg
     1081 ggtgcggttt gccatcatca gccaacctgt gggcgctcgg tgattgttga gcacaacggc
     1141 gacgtttacg cctgcgatca ctatgtttat ccgcaatatc ggctggggaa tatgcatcag
     1201 caaacaattg cagaaatgat cgattccccg caacagcagg tgtttggtga agataaattt
     1261 aagcaattac cggcgcagtg tcgcagttgt aacgtgttaa aagcgtgctg gggaggctgc
     1321 ccgaaacacc gcttcatgct cgatgccagc ggcaaaccgg ggctgaatta tttgtgtgcc
     1381 gggtatcagc gttatttccg ccatctaccg ccatatctta aagcaatggc tgatttgctg
     1441 gcgcacggtc gcccggccag tgacattatg catgcgcatt tgctggtggt gagtaagtaa
     1501 aaaagcggcc attttgtggc cgcctgcggt tgattgccgg atgcggcgta aacgccttat
     1561 ccggcctaca tgatcgtgca aattcaataa attgcagcgt tctgtggata ctggtttggg
     1621 tgatcgtatc cggcaaagcc tttgcgtgcg gtggcagttg acggggtatc tgctgcgctg
     1681 gccatgagag gaagagcggc ggcgacagca acaacaagaa gtttgggtga aaacgaaaat
     1741 tccatgcaaa atgctccggt ttcatgtcat caaaatgatg acgtaattaa gcattgataa
     1801 ttgagatccc tctccctgac aggatgatta cataaataat agtgacaaaa ataaattatt
     1861 tatttatcca gaaaatgaat tggaaaatca ggagagcgtt ttcaatccta cctctggcgc
     1921 agttgatatg taaggcaggt ttattatatc gcgttgatta ttgatgcttt ttttagtttt
     1981 aacggcaatt aatatatgtg ttattaattg aatgaatttt atcattcata ataagtatgt
     2041 gtaggatcaa gctcaggtta aatattcact caggaagtta ttactcagga agcaaagagg
     2101 attacagaat tatctcataa caagtgttaa gggatgttat ttcccgattc tctgtggcat
     2161 aataaacggg tagatgctca ttccatctct tatgttcgcc ttagtgcctc ataaactccg
     2221 gaatgacgca gagccgttta cggtgcttat cgtccactga cagatgtcgc ttatgcctca
     2281 tcagacacca tggacacaac gttgagtgaa gcacccactt gttgtcatac agacctgttt
     2341 taacgcctgc tccgtaataa gagcaggcgt ttttttatgc atcagaaagg tcccggaggt
     2401 gcttgcctcc gggtgagaag gaactactgt ggtgggttgt tctgcaacgt taacatcaaa
     2461 ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca gtctgtccag cgcgtcggca
     2521 agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ccgcgcggaa ggcgagcgat
     2581 gcttcctgcc attctccgtg cttcatcagt gactggccca gtgtgctcca caacagcggg
     2641 cgatcgccga cgtttttgat ctgctggcgc agcacttttt ccagctgttc cggattgttg
     2701 gttttcagtc gaggaatcgg cagcagtagg cgatcgtcgt actggcgttt caggccatcg
     2761 atgataattt gctgggcagt atcatgatcg tcacattcaa taagatgttc cgccattgcc
     2821 acctgcaacg ctacctgatg acgcgttttc cggctttggt ttttccacca gttacgcaaa
     2881 ccttcactac cgttatcggc acgcgcctga tccatcaggc caatccatgc ctgttgttcc
     2941 agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg gatgatatcc
     3001 agcagcgaac tccatgcacc tgtgcggata tacgcctgtt ccgccagacg taatacttcc
     3061 ggatggcgtg gcgtaacttc cagcagctta tccacgccgt ggcgtgcagc atggttttca
     3121 ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc attgccggcc
     3181 agctctgctg cgcgttccag atgttggttg gcgcgtgctt catcaccacg ttgttgcgcg
     3241 gcttcggcag ccagtagata gttcaccacc ggttgttccg cgtgatcggc atttttcgcc
     3301 atcagctttt caacttgctg ataatcgcct tccgccagtt tcagcagcgc ctgttcggtc
     3361 tgcttacggg cacggcgacg cttacgtccg acaaaccacc cacgggtgtg tgcgcctgtg
     3421 cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc cagaatcaaa
     3481 ataatcgcca ggcccgtgac gctggtttcg atattgtagt tgtcagtctg gatcagcacg
     3541 taaccctgat gaccggcaat catcgggcca accactatcc ccgcaatcag caaaacaaag
     3601 agtaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga gtatcacctt
     3661 gcggtgcagg tgcaggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt
     3721 ccgttgtccc cgctgccggt tgtgccagca ggttacgcac gcgggtctgc atcagttttt
     3781 ccagcatcgc ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta
     3841 actggtccac ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagctc
     3901 gtacccaggt ggagacgttc tccagcgcct ggcgataagt ctcttcctgg tgacgcggta
     3961 cagcttgtgc tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat
     4021 ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca
     4081 taaagttctg ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct
     4141 cttcaccgtc ggaatccatc ggcgaaccat cgctgtcatt atcggccaga cgcaggttat
     4201 ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc gtcataatcc acctgcgaaa
     4261 ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt
     4321 cattcatatc cgccaggctg gcatctgcac ttttcagcaa cgctgcagcg gtcgtgacgt
     4381 cctgatcgct ccacagcttc cgtccggcga gtttcaccag aaaatcggcc tgagccagca
     4441 gccaggtttt agcatcgctg ccggaaatgg tggcgacctt ttgttggact tcatccaact
     4501 gttttgccag cgtttcttgc tgacgattcg cctgcttaag ctgtgcagct tgttgcttaa
     4561 taatgccttc cagctcggct ttttggctct cctgggcttt ttgcaatgcc gtcagttggt
     4621 tagccagggc atcgctggtg gcggtctgat tgacggcctg ttgtttaccc cagccataca
     4681 aaccgacacc cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcagtat
     4741 tgttcttact ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccctgg
     4801 tctcttcaac cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga
     4861 gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt
     4921 gccagcccag ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagt
     4981 gtagtaacca gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt
     5041 caccgctggt aacaacgacc atcgtcacct cgcgggattg ccagcgcatc gcttcttctg
     5101 caccatcgta atggattgcg catcgttgat aacattcaca aaaagtgacc tcagcaccgc
     5161 gcgctgtcag ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac
     5221 gtttgcccgc aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat
     5281 cctgcgggta gagaatcttc tgtccgctta cggtatgtag cgccagtgcg gtggtgcgtc
     5341 caatggcgaa ataatcaggt agtcggggcc atttacgatc ttgctgatgc agctgtgatt
     5401 gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgccg
     5461 ccagttgatc agcaagttgc ggtaattgtc gacccggaga aaactcaatc agcggaaaat
     5521 gccaggccac ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg
     5581 ggcgggtgac caggatactc atgccggggc gtctccgtta tagacttcag cgaggatctc
     5641 gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcattttg
     5701 cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc
     5761 acgcagccag atttcaccat caataagctc ggcgtagcta ccaattggca cctgacatcc
     5821 accttcgaga cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg
     5881 attcagcgcg gcaagcagct cgcgagtgcg cgtatcatca aggcggcatt caataccgac
     5941 tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt aacgcggcgc gaatgcggga
     6001 ctccagacct aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt
     6061 atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg
     6121 gcggcgttca gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc
     6181 cggtaacgca tccagactgt cataattatt ggacacaaag gcatcgcgag gatcttcacg
     6241 ctcacaaata gtgaccaacc ccagaccttg cgggaattca accggcacat ctttcattga
     6301 atgtacggcg atatcggcgc gattctccag gagcgcgact tccagctctt ttacaaataa
     6361 gccttttccg cctactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat
     6421 cggtaccagt tcaaccacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg
     6481 tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat
     6541 gcttgttacc gtcattatca tccgtggtcc atcctaacat ccttgccaga gtgatgtcag
     6601 tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct
     6661 gacaggcgtt tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg
     6721 tatgtagcgc atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga
     6781 ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg
     6841 acatacgaaa tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg
     6901 acgggtagca aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac
     6961 tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc
     7021 aacaggtcta cagtctactg ccgacattgt tgcactatca ccatccgcta atgccgggtt
     7081 accttgatgg taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc
     7141 actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt acaggaccca ccgaaaggtg
     7201 agcttccaat tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt
     7261 cctctgacct ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt
     7321 tactacaacg taaatgtagt ctgctggaaa gctgggccgc ctcgctgggt gtggaagtca
     7381 gcttcttcct gattgatgaa aaccgcttcc gtcataatga aagcggcagc ctggggggcg
     7441 aagattgtgg ctccacccag catatactgc tgcttgacga attttatcgt accgccgtgc
     7501 gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg
     7561 acgactatgt gatgacgctt tacgcgcagg gcgtgctgac gccaaatgaa tggctggatc
     7621 tcggtggctt aagctcgctt tctgctgaag agtactttgg tgccagcctt tggcagctct
     7681 acaagagtat cgattcccca tacaaagcgg tactgaaaac actgctgctg gaagcctatt
     7741 cctgggaata cccgaaccca cgtctgctgg cgaaagatat caaacagcgt ttgcacgacg
     7801 gcgagattgt atcgtttggt ctcgatccat actgcatgat gctggagcgt gttactgaat
     7861 acctgacggc gattgaagat tttacccgtc tggatttagt acgtcgctgc ttctatttaa
     7921 aagtgtgcga aaagctcagc cgtgaacgcg cctgcgtagg ctggcgtcgc gcagtgttga
     7981 gccagttagt gagcgagtgg ggttgggacg aagctcgtct ggcaatgctc gataaccgcg
     8041 ctaactggaa gattgatcag gtgcgtgagg cgcacaacga gttgctcgac gcgatgatgc
     8101 agagctaccg taatctgatc cgctttgcgc gtcgcaataa ccttagcgtc tccgccagtc
     8161 cgcaggatat cggcgtgctg acgcgtaagc tgtatgccgc gtttgaagca ttaccaggta
     8221 aagtgacgct ggtaaacccg cagatttcac ccgatctctc ggaaccgaat ctgaccttta
     8281 tttatgtgcc gccgggccgt gctaaccgtt caggttggta tctgtataac cgcgcgccaa
     8341 atattgagtc gatcatcagc catcagccgc tggaatataa ccgttacctg aacaaactgg
     8401 tggcgtgggc atggtttaac ggcctgctga cctcgcgcac ccgtttgtat attaaaggta
     8461 acggcattgt cgatttgcct aagttgcagg agatggtcgc cgacgtgtcg caccatttcc
     8521 cgctgcgctt acctgcaccg acaccgaagg cgctctacag cccgtgtgag atccgccatc
     8581 tggcgattat cgttaacctg gaatatgacc cgacagcggc gttccgcaat caggtggtgc
     8641 attttgattt ccgtaagctg gatgtcttca gctttggcga gaatcaaaat tgcctggtag
     8701 gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt gcgtacgctg cacttcaacg
     8761 gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg caaaatgcat caggacgccg
     8821 caccgccaga tagcgtggaa gtcttctgtt atagccagca tctgcgcggg ttaatccgta
     8881 ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg cctttccagc acccgccagg
     8941 aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac ctgggggttg ttcttcgaac
     9001 gcctgaatgt atcggtacaa aaactggaaa acgccatcga gttttatggc gctatttcgc
     9061 ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg
     9121 tggtggacgg ctttgccagc gaagggatca tccagttctt cttcgaagaa acgcaagacg
     9181 agaatggctt taatatctac attctcgacg aaagcaaccg ggttgaggtg tatcaccact
     9241 gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg cttctactcg tcatcgcacg
     9301 atcgcttcac ctacggctca agctttatca acttcaacct gccgcagttc tatcagattg
     9361 tgaaggttga tggtcgtgaa caggtgattc cgttccgcac aaaatctatc ggtaacatgc
     9421 cgcccgccaa tcaggacaac gatacgccgc tattacagca gtatttttcg tgatgagcgt
     9481 gccggaaagc gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgct
     9541 tgctgtgtcg ccgcctgttc cagcaaatcc cagaaggttt cgccgctgcg atcgcaaatc
     9601 cattcatcgc cttttaggtc aaaatggtag ccgccctgtt tggttgccag ccacacctgg
     9661 tgcagcggct cctggcggtt aataatgatt ttgctgccgt tttcaaaggt aatggtcagt
     9721 acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat cccagtcgtc caggcgttct
     9781 tcaatggtca gccagagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta
     9841 tcctgtcttt aagtgatggc ggcagtatag cggtatgggg tcagggcttc aaagtttgcc
     9901 cgtctgttgc tgcgttccgg catgagtcat ccgtcaacgg aataataatg tctctgttgt
     9961 gaagttcacg cacattctct tctctgacgt gtgttttcgc cgggcagggg gagggcgtaa
    10021 tccttacctc tgtatcggtc catgtataaa cattgcgatc gtcataagcg aagcgatcgt
    10081 ttttagttag cggaccgtta actggaataa tttgcacgct ctttgaccgt aattggcgga
    10141 taaaataagc atgctgtttg ccgctattaa atgagcgatc aatgaccagg tctggcgtat
    10201 ccagcgggat tatctcaagc tggtcttcac caaatgtcga taattttgtt acatacagcc
    10261 gatctttata ttgcgcaata ttgccagtaa tcagatggaa ttgttcaggt tcaatatctg
    10321 gtaggtctat ccacttgctc cactgacggt cggacgtgag ctgtttacgc cagcgaacgc
    10381 gactttcccc tacttcaaaa gagacgtcat catctacggg tatcgctttg ccagacaatt
    10441 gcccaaaggg ataacgatgc atacccttat tgtcgcgaaa aaccagcagt gaatgaggta
    10501 tccagcgaat tatctcaaat gagtctgcat tcgccgtaag gtgaacgcca tttaaataaa
    10561 tattctgccc actgcggata agcagatcat tggaatacaa atttctgttt acactcataa
    10621 gacgtggatt aataccccac catctttttt ctaatacagt aacattactg ctgctcccct
    10681 gacgtcgacc ggacaaatag agatttttac tatccaccag cgtggtagag ttaccttcga
    10741 tagcttttag tgtcgccaga tcaacacgtg acgcgccgct attactttca gtgcgctgtc
    10801 cgtcaaaata gaggctatat ttatcgaccg cgaagcggcc catagcctga aagctggcaa
    10861 tatcgacagt gggggttccc ggtggattgc taacgatttt gccacgccag agaataacgc
    10921 ggccgtctgt cacccagtgg tattcctcat tttttatgct tatttcatcg gtgggtatat
    10981 agccttccac caactcccgc tccccggata tatttgtcac aaggcgcaaa tcggccctgc
    11041 tcatctccgg aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca
    11101 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
    11161 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcgcctcatt
    11221 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
    11281 gtaatttacg ccatagccat atcccgaatg ttgttccttc cagattaagc gaacttgttt
    11341 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
    11401 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
    11461 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacat gtaaaccgga
    11521 atggtatggc cacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
    11581 acgtttgttg ataataagat tgggatcatc aagtttcacg atttccagct tgcggtcacc
    11641 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    11701 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    11761 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    11821 atcccactcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    11881 ggtcaagagt gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    11941 ctgttcgccg ttgatcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    12001 tggcatagcc acacagggat taaacgcggt agaaaacttt cctcgctgat cccatgactt
    12061 ttgtgccagc acacggaaac tatcgggatc tgcgaggcga tgcccattga tatacaagaa
    12121 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    12181 ctgatgcagt gttttgatat ctaagcgatt tcccccgcca ttgttatcgg tgcgcttgcc
    12241 ttcgaaatag agactgtgtt tatctgcggc aaaatcaccc cacgccttga acgaggcgac
    12301 atcgacggga ggtttgtctg gcgggttttg cacaacttca cccgcgtaga ggatatagcg
    12361 accatcgcta tgccaggagt aatgaggata ccaggcctgt gcgccttcat agaggtagtc
    12421 ggttgtcaga tcagtcagag gaggtacttt atcccatttg tctagtgttg tcgggtctat
    12481 taataaattg ggtaaacggc gcaaactttt gtaattcacg tcaggaagag aaagatttgt
    12541 ttctttataa gggcgcaggt ataccacgcc ttttccctgt ttctggtaaa tattatgttc
    12601 aggcggatca agccgacatc cccatgccga cgataaaaac aatgaacaag acaacaaaca
    12661 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgccgatca cgaaactacc
    12721 agcagaacat aattcctcgc acgtaagcgt aacttttgcg gcaaatcgtc acttttcaga
    12781 atctataaag gccatcgcta ttttctgata gttgctgggg taaacttgag gttttcattc
    12841 acgatataaa tacgagctac tgcgccatgg tgtgactgat cacgttaaaa tgtgccggat
    12901 tgacgccggg aagggtgtca acatgcgact ctgcccaccg ttacctactt tattgtcttt
    12961 cgcgtgttgt acttaggtcc caccaccggt atctaacatt ccccacatta ttacgctttc
    13021 tgccgaacgt gatttttata gaaatctatc aattcatttt ccctgtacca taaagtttat
    13081 ctttcacatc ctgccgaacg cgggtcttgt accccccgtt gtgaagatct tcatacggag
    13141 aaaaagaatc tttactccac cccagcagca ctttatcgcc aggcataaaa tgcacatgca
    13201 gaaaatcttc tccccattct cttttgggga gaggtaacgt catattccgt ttctccagac
    13261 gcattccagc attatactgt gtacgagtag tacttaatgt ccagataacc tctgccgtat
    13321 ccccactaac atcaccacaa caggttgttg aaccgccacc atttgcaaaa gcattcgcgc
    13381 ccgccacgcc gttaacactg aatcccagta tcgggcggtc aatttcactg tggatttcca
    13441 gaatcactcc acctcttggt cctccccata ctgcctgtcc tatattccac aacacgacca
    13501 gtaccggaac gacaattaac gtccgccata cccagcgccc ccaccgggca taaccgcgcg
    13561 taagcttttg gtcaaatttt tccagttggg taaatagccc catcagattt tattccctct
    13621 tatcaccagg ttggcaaagt tgtatgtcac tggcgactaa ggtcagacaa caaaattgca
    13681 tggtgcgcca ctcttttaaa agctccatgc cgacgataag aacaacgaac aagccaacaa
    13741 acaaactaca ttgcgatagt gcatagagcc atcctggcgc gaggtgccga tcacgaaact
    13801 accagcaaaa cataaatccc cacgagtaag cgttatactc gcagcatttc ctcacttttc
    13861 agacttcata aagagtcgct aaacgcttgc ttttacgtct tctcctgcga tgatagaaag
    13921 cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag gcactcactg
    13981 tattaattac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc tatttcccgc
    14041 ctgcagataa aaacgcaccg ccgccgacca aaccggtaga gacgcaaacg caatccacgg
    14101 tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat tactaaaagt
    14161 cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg catggccttg
    14221 gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca ccggagctga
    14281 ttcgtcgcct ggctgatcgg cacctggggg tagggtttga ccaactgctg gtggttgagc
    14341 cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat ggcagtgaag
    14401 tggcgcagtg cggcaacggt gcgcgctgct ttgcccgttt tgtgcgtctg aaaggactga
    14461 ccaataagcg tgatatccgc gtcagcaccg ccaacgggcg gatggttctg accgtcaccg
    14521 atgatgatct ggtccgcgta aatatgggcg aacccaactt cgaaccttcc gccgtgccgt
    14581 ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag acaatcttat
    14641 gcggcgtggt gtcgatggga aatccgcatt gcgtgattca ggtcgatgat gtcgataccg
    14701 cggcggtaga aacgcttggt cctgttctgg aaagccacga gcgttttccg gagcgcgcca
    14761 atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt tatgagcgtg
    14821 gggcaggaga aacccaggcc tgcggcagcg gcgcgtgtgc ggcggttgca gtagggattc
    14881 agcaaggttt gctggccgaa gaagtacgcg tggaactccc cggcggtcgt cttgatatcg
    14941 cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat gtctacgacg
    15001 gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca cggagcttga
    15061 tgaccgggcg gttgtcgatt atctgattaa aaatcctgag ttttttatcc gtaatgcgcg
    15121 cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggc accgtttcgt tggtcgagtg
    15181 gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg cgctgttgat
    15241 ggaacaggct atcgccaacg aaggcctgtt ttatcgccta ctctacctgc agcgcagtct
    15301 caccgccgcc agcagtctcg acgatatgct gatgcgcttt caccgctggg cgcgcgatct
    15361 cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag gtgcgccgtc
    15421 gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta ttcagcgttt
    15481 ggggcaggaa cagcactatc ttgggccgct taacggacca gagctgctgg tggtgctacc
    15541 ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg ctgatttggg
    15601 tgtcgtgctg tttaccagtc gcgatgccag tcactatcaa caagggcaag gaacgcagtt
    15661 acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg aacgcgtatg
    15721 accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga gcgccagctt
    15781 agcccgataa ccctgcttaa ctaccagcgt cagcttgagg cgatcatcaa ttttgccagc
    15841 gaaaacggcc tgcaaagctg gcagcaatgt gatgtgacga tggtgcgcaa ttttgctgta
    15901 cgcagtcgcc gtaaagggct gggagcagca agtctggcgt tacggctttc tgcgctacgt
    15961 agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc gaaaggtgtt
    16021 tcggcaccga aagcgccgcg tcatctgccg aaaaacatcg acgtcgacga tatgaatcgg
    16081 ctgctggata ttgatatcaa cgatcccctc gctgtacgcg accgtgcaat gctggaagtg
    16141 atgtacggcg cgggtctgcg tctttctgag ctggtggggc tggatattaa acacctcgac
    16201 ctggagtctg gtgaagtgtg ggttatgggg aaaggcagca aagagcgccg cctgccgatt
    16261 ggtcgcaacg ctgtggcgtg gattgagcac tggcttgatt tgcgcgacct gtttggtagc
    16321 gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg taatgtgcag
    16381 aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca cccacataaa
    16441 ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg tggtgtgcag
    16501 gagctactgg gtcatgccaa cctctccacc acgcaaatct atactcatct tgattttcaa
    16561 caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa ataatgcgtt
    16621 tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat accctttacg
    16681 ataaccgtcc ggtgattttg cgcaccgagc gagaggcgct tacctttgtg caaaattatc
    16741 atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag gcggtacggg
    16801 aagtggaacc cgagatttat cacgacgtga cgcgctggcg ttttcgttcg attgaacaag
    16861 cgatgctcga cgccgggctg agtgccgaag aagccagtgc aggcgcacac gcagcaatga
    16921 tcaactttgc caaatggcgc agccgaatcg acgtcccgca gcaaactcac gacaccttaa
    16981 aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgcc cagccggaac
    17041 tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac gggcgctcaa
    17101 aaccgttcag cgatatgtac tttctggctg cggaaaaact caacgtgccg atcggcgaga
    17161 tcttacatgt tggggacgat ctcaccactg acgtgggtgg ggcaattcgc agcggaatgc
    17221 aggcctgttg gatcagaccg gaaaatggcg atctgatgca aacctgggac agccgtttac
    17281 tgccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa tcagcaaatc
    17341 tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa cctattttta
    17401 cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca aacagcgcga
    17461 agcggtggcc gcgccacgca gcaaccttct ggtgctggcg ggcgcgggca gtggtaagac
    17521 gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct cgccatactc
    17581 gattatggcg gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc gtatcgggca
    17641 gctgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc tggcgcaccg
    17701 tctgctgcgt gcgcaccata tggacgccaa tctgccgcag gattttcaga tcctcgacag
    17761 cgaggaccag ctacgcctgc tcaaacgcct aatcaaggcg atgaacctcg acgagaagca
    17821 gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag gcctgcgtcc
    17881 acatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg tgtatcaggc
    17941 gtatcaggaa gcgtgtgacc gcgcgggcct ggtggacttc gccgagctgc tgctacgtgc
    18001 tcacgagttg tggcttaaca agccgcatat ccttcagcac tatcgcgaac ggtttaccaa
    18061 tatcctggtg gacgaattcc aggataccaa caacattcag tatgcgtgga tccgcctgct
    18121 ggcgggcgat accggcaaag tgatgatcgt tggcgatgac gatcagtcaa tctacggctg
    18181 gcgtggagcg caggtggaga atattcagcg tttcctcaat gatttccccg gtgctgaaac
    18241 tatccgtctg gagcagaact accgctctac cagcaatatt ctgagcgccg cgaacgccct
    18301 gattgaaaac aacaacgggc gtttgggtaa aaaactgtgg accgatggcg cggacggtga
    18361 gcctatttcc ctctattgcg cttttaacga actcgatgaa gcgcgttttg tggttaaccg
    18421 catcaaaacc tggcaggaca acggcggggc gcttgccgag tgcgccattc tctaccgcag
    18481 taacgcccag tcgcgtgtgc tggaagaagc actgttgcag gcgagtatgc cgtaccgtat
    18541 ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gatgcgctct cgtatctgcg
    18601 cctgattgcc aaccgtaacg acgacgcggc ctttgagcgt gtggtgaata cgccaacgcg
    18661 gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc gccagttaac
    18721 actctggcag gcatgtcgtg aactgttgca ggaaaaagcc ctcgccggac gtgctgccag
    18781 cgccttacag cggtttatgg aactgatcga cgccttagcg caggaaactg ccgatatgcc
    18841 gctgcatgta cagactgacc gggtaattaa agactccggc ctgcgtacca tgtacgagca
    18901 ggagaagggc gaaaaaggtc agacgcgtat cgaaaactta gaggaactgg tgacggcaac
    18961 gcgccagttc agctacaacg aagaagacga agatttaatg ccgctgcagg cgttcctatc
    19021 ccatgcggcg ctggaagcag gcgaagggca ggcggatacc tggcaagatg cggtacaact
    19081 gatgaccatg cactcggcga aaggtctgga gttcccgcag gtgtttatcg tcggtatgga
    19141 agagggcatg ttcccaagcc agatgtcgct ggatgaaggc gggcgtctgg aagaagaacg
    19201 ccgtctggcc tacgttggcg taacccgcgc gatgcagaaa ctgacgctga cctacgcgga
    19261 aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta ttggcgagct
    19321 gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc cggtcagcca
    19381 tcagcggatg ggtacgccga tggtcgagaa cgacagcggc tacaagctcg gccagcgcgt
    19441 acgccacgct aagtttggtg aaggcaccat tgtcaatatg gaaggcagcg gtgagcatag
    19501 ccgtttgcag gtggcatttc agggccaggg tattaaatgg ctggtggcgg cttatgcccg
    19561 gctggagtcg gtgtaacgtt gccggatgcg gtgctgcgca ccttacttgg cctaaaaaat
    19621 cattcagatt caataaattg caacgtcatg taggccggat agggcgttta cgccgcatcc
    19681 ggcatctgcg ccatcttcag tatctgacac aaaactatcg ttttaacctt tccgctctac
    19741 ggaaatcatg gtgacaaaag gatagcgttg ccacgggatt gccccgcctt tcatatacat
    19801 atgtgaaatc gtgccatcaa gataaagcag ctgctcaacg ttcagtttcg ctttggcata
    19861 acaggcaaaa tcataaaaat ttgtcgcctg ctggctcaac aaaaacacgg cattcccatg
    19921 tttattaatc ccaacaccgt tacgaatttt acgtgaggcg acgttgggat gaatacgcgg
    19981 attaattaca ctgttttcca tcaacattgg ccctgactgc accgcaaact gaatctcttt
    20041 actggttttg aaggcatcca gacgaacgat gccgacttta tctcccgcga cataaaacac
    20101 gccgccagga cggataaaga aattcccttc acctgaagcg agatttaacg ccaccttctg
    20161 ctgaccgttt tcgatgtaca aaccgagcgg cgcatagctt tcatcataga tgccgccgtt
    20221 catcgccatc tgcacctgac cctgactatt catatccgcc agcagagcat gtaaagttcc
    20281 ccacgcttcg ccattggctt tttgccagta cattttcacc cgctctgttt gaggattaac
    20341 ggtatacgcc tgtacggtca gcgtcggatc tgagagtgcg caatcatcag cggcaacagc
    20401 aaacaaggga agtaaggtga gggtgagaaa aatccgtttg agattcaagg tgatcattcc
    20461 tttaccaatg agtagccgat gcgccattat aggtcctgga tgttggattt ttttatcctg
    20521 ttagcgacct tgacgagtac caaaaagcgc gaagttcaac tattgttctg tggtgttctg
    20581 ttgcgtgttg acggcaaaat tttgctggcg taacatgcgc gcacgatcac tctaagagga
    20641 cattcgcctt ggacacaccc agtagatact ggctcactat cctgtcatcc aggatcaact
    20701 cctaaggcta tccctttttg ctgatagcct tagcggttgt cagcgacctc aatttttccc
    20761 gtcgcgctga gtcaggctgt ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg
    20821 aactgtccga tattttaagc attgggagtc ccggtcatgc tgagcgcatt tcaactggaa
    20881 aataaccgac tgacccggct ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg
    20941 attgatcttg tcgaaccgga cgacgacgag cgactgcgcg tacaatctga acttggccag
    21001 agcctggcaa cccgcccgga actggaagac atcgaagcat cggcacgttt ctttgaagac
    21061 gacgacggcc tgcatattca ctccttcttc ttctttgaag atgcggaaga tcacgccggt
    21121 aactccactg tggcatttac catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa
    21181 ctgcccgctt ttcgtctgta tcgtatgcgt gcccgtagcc agtcgatggt agacggtaac
    21241 gcctacgagt tgctgctgga tctgttcgaa accaaaatcg aacagttggc agatgaaatt
    21301 gaaaatatct atagcgacct ggagcagttg agccgggtga ttatggaagg gcatcagggc
    21361 gatgagtacg acgaggcgct ctccacgctg gcggaactgg aagatatcgg ctggaaagtt
    21421 cgcctgtgtc tgatggatac ccagcgcgcg ctcaacttcc tggtacgcaa ggcgcgttta
    21481 ccgggtgggc aactggagca ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg
    21541 cataacgaat ccctgttcca gaaggtgaac ttcctgatgc aggcggcgat gggctttatc
    21601 aacatcgagc agaaccgcat catcaaaatc ttctcggtgg tatccgtggt gttcctgcca
    21661 ccgacgctgg tggcttccag ctacgggatg aactttgaat ttatgccaga gttgaagtgg
    21721 agtttcggct acccaggcgc gattatcttt atgatcctcg cgggcctggc accgtatctg
    21781 tactttaagc ggaagaactg gttgtaatat gagtgccgga taaagctatt tttttatccg
    21841 gcttatttct aaaaattgtc ttttttaaag tcatgcggat tgaaaactcg ctctatttta
    21901 ttttcttgtg ttaattgata ctcagtaaaa tcgttaat
//
LOCUS       DAZKGS010000068        21692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKGS010000068 DAZKGS010000000
VERSION     DAZKGS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..307
                     /locus_tag="ACHOQY_002582"
     CDS             <1..307
                     /locus_tag="ACHOQY_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP9678661.1"
                     /translation="ELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIWNRER
                     ISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQGEGA"
     gene            304..927
                     /locus_tag="ACHOQY_002583"
     CDS             304..927
                     /locus_tag="ACHOQY_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9678662.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            2000..2323
                     /locus_tag="ACHOQY_002584"
     CDS             2000..2323
                     /locus_tag="ACHOQY_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFP9678663.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2307..2783
                     /locus_tag="ACHOQY_002585"
     CDS             2307..2783
                     /locus_tag="ACHOQY_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9678664.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            2780..3217
                     /locus_tag="ACHOQY_002586"
     CDS             2780..3217
                     /locus_tag="ACHOQY_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9678665.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            3423..3941
                     /locus_tag="ACHOQY_002587"
     CDS             3423..3941
                     /locus_tag="ACHOQY_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9678666.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            4225..4596
                     /locus_tag="ACHOQY_002588"
     CDS             4225..4596
                     /locus_tag="ACHOQY_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFP9678667.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            4700..4942
                     /locus_tag="ACHOQY_002589"
     CDS             4700..4942
                     /locus_tag="ACHOQY_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFP9678668.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            5022..5447
                     /locus_tag="ACHOQY_002590"
     CDS             5022..5447
                     /locus_tag="ACHOQY_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFP9678669.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            5444..6856
                     /locus_tag="ACHOQY_002591"
     CDS             5444..6856
                     /locus_tag="ACHOQY_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFP9678670.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            6859..8985
                     /locus_tag="ACHOQY_002592"
     CDS             6859..8985
                     /locus_tag="ACHOQY_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9678671.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYRKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVVVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            8999..9883
                     /locus_tag="ACHOQY_002593"
     CDS             8999..9883
                     /locus_tag="ACHOQY_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFP9678672.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKHAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            9895..11166
                     /locus_tag="ACHOQY_002594"
     CDS             9895..11166
                     /locus_tag="ACHOQY_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFP9678673.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            11209..11394
                     /locus_tag="ACHOQY_002595"
     CDS             11209..11394
                     /locus_tag="ACHOQY_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678674.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            11369..11851
                     /locus_tag="ACHOQY_002596"
     CDS             11369..11851
                     /locus_tag="ACHOQY_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFP9678675.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            11860..13278
                     /locus_tag="ACHOQY_002597"
     CDS             11860..13278
                     /locus_tag="ACHOQY_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFP9678676.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            13278..14126
                     /locus_tag="ACHOQY_002598"
     CDS             13278..14126
                     /locus_tag="ACHOQY_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFP9678677.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            14126..14581
                     /locus_tag="ACHOQY_002599"
     CDS             14126..14581
                     /locus_tag="ACHOQY_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP9678678.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            14584..15276
                     /locus_tag="ACHOQY_002600"
     CDS             14584..15276
                     /locus_tag="ACHOQY_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9678679.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            15286..16692
                     /locus_tag="ACHOQY_002601"
     CDS             15286..16692
                     /locus_tag="ACHOQY_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP9678680.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            16692..18536
                     /locus_tag="ACHOQY_002602"
     CDS             16692..18536
                     /locus_tag="ACHOQY_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP9678681.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(18551..19036)
                     /locus_tag="ACHOQY_002603"
     CDS             complement(18551..19036)
                     /locus_tag="ACHOQY_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678682.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(19107..19373)
                     /locus_tag="ACHOQY_002604"
     CDS             complement(19107..19373)
                     /locus_tag="ACHOQY_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9678683.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            19495..21618
                     /locus_tag="ACHOQY_002605"
     CDS             19495..21618
                     /locus_tag="ACHOQY_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFP9678684.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHLPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
BASE COUNT         6155 a         4944 c         5394 g         5199 t
ORIGIN      
        1 tgaacttccg ttaatcatcg aactggtgag caaagataaa aaatatgtca tctgccacgc
       61 cgattatcct tgtgacgaat acgagtttgg aaagccagtt gatcatcagc agttaatttg
      121 gaaccgcgaa cgaatcagca actcacaaga cgggatcgtg aaagaaatca aaggagcgga
      181 catgttcatc tttggtcata cgccagcagt gaaaccactc aagtttgcca accagatgta
      241 tatcgatacc ggcgcagtgt tctgcggaaa cctcacattg attcatgtac agggagaagg
      301 cgcatgagac tcgaaagcgt agctaaattt cattcgccaa aaagcccgat gatgagcgac
      361 tcaccacggg ctacggcttc tgactctctt tccggtactg atgtgatggc tgctatgggg
      421 atggcgcaat cacaagccgg attcggaatg gctgcattct gcggtaagca tgaactcagc
      481 cagaacgaca aacaaaaggc tatcaactat ctgatgcaat ttgcacacaa ggtatcgggg
      541 aaataccgtg gcgtggcaaa gcttgaagga aatactaagg caaaggtact gcaagtgctc
      601 gcaacattcg cttatgcgga ttattgccgt agtgccgcga cgccgggcgc aagatgcaga
      661 gattgtcacg gtacaggccg tgcggttgat atagcaaaaa cagagcagtg ggggagagtt
      721 gttgagaaag agtgcggaag atgcaaaggc gtcggctatt caaggatgcc agcaagtgcc
      781 gcatatcgcg ctgtaacgat gctaatccca aaccttactc aacccacctg gtcacgcact
      841 gttaagccgc tgtatgacgc tctggtggtg caatgccaca aggaagagtc aatcgcagac
      901 aacattttga acgcggtcac acgttagcag catgattgcc acggatggca acatattaac
      961 ggcatgatat tgacttattg aataaagttg ggtaaatttg acccaacgat gggttaattc
     1021 gctcgttgtg gtattgagat aaaaagaggc ggcgcttact accgattccg cccagttggt
     1081 cacttcgacg tatcgtctgg aactccaacc atcgcaggct gagaggtctg caaaatgcaa
     1141 tcccgaaaca gttcgcaggt aatagttaga gcctgcataa cggtttcggg attttttata
     1201 tctgtgtaac aggtaagagc attctccctt atggggcttg gcttaaatgc accgagtgct
     1261 cttatcgttg tggcagcaca acgatagttt tcgtcagagt tggcgacttt gcggtttttt
     1321 agaaactgac cacaaagata aatgcaaacg atgatgttgt tctgatggcg gcgtaatagc
     1381 ctgtaagtca gcaaggtctt ccgactcctt gtaaacaaat tcggcgcact ggcccggtgt
     1441 gattaataat gggcacacaa caggtaagag cattaaagaa ctggcaaaga gcttaacggt
     1501 ctgcgaaagc atttcttagt ggcacaactg gccggtacaa ctgagtgctc tttccggtgt
     1561 ggtgaatgcg caggctgatg cgcgcaggag agcttcggaa gaacaaggtg cctgtataca
     1621 agccggagat cagcgccggc caccacagcc aaatccaccc agagcaaaac cgttgttcat
     1681 ccttaccatt cactcagtat tttgggctac accatcagcc cattttttaa agcgtacttc
     1741 caccaagaac cagacctaac caactcattg ctgacactct gtggatacgg ttgtctagta
     1801 cgctttaaaa aagaaaaccc agcatcaatg gctgggcttc gtgatatgag cggcatgtat
     1861 tgttggcgca atccacgcct gatttgctca tgaatgcggt cacgaacaaa cccgttacaa
     1921 atcaaccgta acccggattt gttcaagcga ccatatccat aattcctaat ttgaacagat
     1981 ccccttctgg gggtaagaca tgaagatgcc agaaaaacat gacctgttag ccgctattct
     2041 cgcggcaaag gaacaaggca tcggggcaat ccttgcgttt gcaatggcgt accttcgcgg
     2101 cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt gtgccattat
     2161 cgcctggttc attcgtgacc ttctcgactt cgccggacta agtagcaatc tcgcttatat
     2221 aacgagcgtg ttcatcggct acatcggtac tgactcgatt ggttcgctta tcaaacgctt
     2281 cgctgctaaa aaagccggag tagaagatgg tggaaatcaa taatcaacgc aaggcgttcc
     2341 tcgatatgct ggcgtggtca gagggaacag ataatggacg gcagaaaacc agaaatcatg
     2401 gttatgacgt cattgttggc ggagagctat tcactgatta ctccgatcat cctcgcaaac
     2461 ttgtcacgct aaacccaaaa ctcaaatcaa cagccgccgg acgttaccag cttctttccc
     2521 gttggtggga tgcctatcgt aagcagcttg gcctgaaaga cttctctccc aaaagccagg
     2581 acgctgttgc gctgcagcag attaaagagc gtggcgcttt gccgatgatt gatcgcggtg
     2641 atattcgtca ggcaatcgac cgttgcagca atatctgggc ttcactgccg ggcgctggtt
     2701 atggtcagtt cgagcataag gctgacagcc tgattgcaaa attcaaagaa gcgggcggaa
     2761 cggtcagaga gattgaggta tgagcagagt caccgcgatt atctccgctc tggttatctg
     2821 catcatcgtc tgcctgtcat gggctgttaa tcattaccgt gataacgcca tgacctacaa
     2881 agagcagcgc gataaagcca catccatcat cgctgatatg cagaagcgtc aacgtgatgt
     2941 agcagaactc gacgccagat acacaaagga gcttgctgat gctaacgcta ctatcgaaag
     3001 ccttcgtgct gatgtttctg ctggtcgtaa gcgcctgcaa gtcgccgcca cctgtgcaaa
     3061 gtcaacgacc ggagccagca gcatgggcga tggagaaagc ccaagactta cagcagatgc
     3121 tgaactcaat tattaccgtc tccgaagtgg aatcgacagg ataaccgcgc aggttaacta
     3181 cctgcaggag tacatcagga ctcagtgcct gaaataattt ttttgcaaat cacaaagtcc
     3241 atttaatgag cctcgcgatg cggggctttt tttacatctg aatttcacag cgcatctcac
     3301 gcgcatatta catcacccga gcctttcaga aagttgagcc tgagaactgc cgtatatggt
     3361 ggcgaccatc tcggggcggc ttttctgtga gacaggctca ctttctaaaa ggtaaagacg
     3421 ctatgaatca tcaattggct aatctcgatt tccgggacat ggtggttgtt tctggtgatc
     3481 gcgtgatcac aacctcccgc aaggtagcag cttacttcga caagcagcat caccacatca
     3541 ttcagaaaat cgaaaagcta gactgttcgg atgaatttct aaccagcaac ttttcgcggg
     3601 ttacctatga acacaagggt aatcagtatg ttgaatatga aatttccaaa gacggtgcga
     3661 tgtacatcat catgtcgttt accggcaaaa aagctgccgc catcaaagag gcgtttatca
     3721 aagcatttaa ttggatgcgt gacaggctga tggagatggc tcactcatac caaagagagc
     3781 acaacgagtt aatgctggag ttcatgaagg aaaaggatgt tgccagtatg tcaggacgct
     3841 tgctgaaccg ctggggcagg atcaaaaaac cgcaactcat agcaagaatc aaaaggcttg
     3901 agcagcaggc gcaaatatcg atccccggac tgccaaagtg accattccaa agcccatcta
     3961 cgggtgggct tgataatgaa accgtgattt acatccccac aatccgggta tgtaaaagat
     4021 agttcaggcg agaacagatt taactaaatc tgtgcaccac cagttacggc agtaccgcga
     4081 aacaacccaa gccagtaagt ggggaaataa cactggcagc cactgaaaga tgaacctcca
     4141 gccttatggc aaaaaagatt ctttgtggtg gcgaactgat ggaaagacat cctaatcaag
     4201 caaccactct acagggtcat aattatgaac gaccagcaaa tcgaaaaaga aatcgttgag
     4261 aaaggcaaaa cggcaccgcg agttacgcca gaccatatcg aaggaattat tgctcaggag
     4321 gcatatttca cagcagagga tggtgccttt ggcaaagcca taaaagcgaa acatactggc
     4381 ggagaggtaa actaccagtc gcacgaatca ctttctctgc tgacgttctg cgtcctggtg
     4441 ctgcgcaacg gcttcaccgt caccggagag agtgcctgtg caagtcagga aaattttgat
     4501 gcagaaattg gtcggaagat tgcccggcag aatgctgtaa acaaaatctg gatgctcgaa
     4561 ggttacttgc tgaagcagaa gctaagcgaa cagtagttat tacaaaagcc attccttact
     4621 gagtggcttt gataatggct tataccctac acgggataac ttaactgata tcccttttaa
     4681 cggataaacg gagccaacaa tggcagagat tattcccatg actgaagaac agaaattcca
     4741 gttagagatt tacaaactgg tcatgaacca gaacgcagcc gcggaggaag catttcagtt
     4801 cattggcact gacgagctga agcttgagct attcaaaatt cacttccagt caggtggcgc
     4861 taattcagat atcacgaccc gcacaatcga agcggtgcgt aaatcgaagg aagcgttaga
     4921 cctgttcact accggagcgt aaacatggca actcaaggtt tcgacaaccc atccaaattc
     4981 cgcgatgaat gggataagca agcagaaggg aaataatcaa tatggcgact gagaaaaaga
     5041 aaggtggtcg cccctctgat tatatgccgg aggtggctaa tgacatttgc gcattgcttt
     5101 cctccggtga gagtctgcgc aaagtttgcg aacgcccagg aatgccgagc aaaacatcag
     5161 tttttcgctg gctggctgaa catcaggagt ttcgtgacca gtacgcgaag gcaacagaga
     5221 ctcgggccga ctctattttc gaagagatat tcgaaattgc tgacgacgta atccctgatg
     5281 ccgccgaggt ggcaaaggca agacttcgcg ttgatacccg caaatgggcg ctggcccgaa
     5341 tgaatccccg taagtatggc gacaaggtaa ctaacgagct tgtcggcaaa gacggcggcg
     5401 caatccagat tgaaacatca ccgatgagca ctctattcgg aaaatgacct cgattaatcc
     5461 tatctttgaa ccgttcattg aggcgcatcg ctacaaagtc gccaaaggcg gtcgaggtag
     5521 cggtaagtca tgggcaattg cgaggcttct tgttgaagcg gcgcgtcggc agcctgtgcg
     5581 tattctttgc gctcgtgagc tgcaaaacag tatcagcgat tcggtaatca ggttgcttga
     5641 agacaccatc gagcgtgaag ggtattcggc tgagtttgaa attcagcgtt caatgattcg
     5701 tcatctcgga acgaatgctg aattcatgtt ctacggcatc aaaaacaacc cgacgaagat
     5761 taaatcgctc gaaggcattg atatctgctg ggtggaagaa gcggaagcgg taacgaagga
     5821 atcgtgggat atcctgattc caaccatccg taagccgttc tctgaaatat gggtaagttt
     5881 caacccgaaa aacatcctcg acgataccta tcagcgattc gtcgtaaatc ctcccgatga
     5941 tatttgcctg ctgacggtga actacaccga caacccgcat tttcctgaag tcctccgtct
     6001 ggagatggaa gagtgtaaac gcagaaatcc gacactgtat cgtcacatct ggcttggtga
     6061 gccggtaagc gcaagtgata tggcaatcat caaacgtgaa tggcttgaag ccgcaaccga
     6121 tgcgcacacg aaactcgggt ggaaagcgaa aggtgcggtt gtttctgcgc atgacccctc
     6181 agatacaggg ccagatgcta aaggttatgc atcgcgtcac ggttcggtag ttaagcgcat
     6241 tgccgaaggt ctgctgatgg acatcaacga gggtgctgac tgggctactt cgctggcgat
     6301 tgaagacggc gctgaccatt acctgtggga cggtgatggc gtcggtgcgg gcctacgcag
     6361 acagacaacg gaagcattct ccggcaagaa aattaccgcc acgatgttca agggcagcga
     6421 atcgccattc gatgaagatg ggccttatca ggccggagca tgggctgatg aagtcgtaca
     6481 gggcgacaac gttcgcacta ttggcgatgt attccgcaat aagcgagcgc aattctatta
     6541 cgcgctggct gacaggctgt atctgacata tcgggcggtt gtccacggtg agtatgcaga
     6601 ccccgacgac atgctgagct tcgacaaaga agcgataggc gagaagatgc tggagaagct
     6661 gtttgcagaa ctgacgcaga ttcagcgcaa attcaataat aacgggaagc tggagcttat
     6721 gactaaggtc gaaatgaagc agaagctcgg tatcccatct cctaacctgg ctgatgcgct
     6781 gatgatgtgt atgcattgcc cggcattggt ccgcgaagaa acagaaatat acgttccctc
     6841 atcctccggt tggtaaacat ggcagagaca ttagagaaaa aacatgagcg gatcatgctc
     6901 aggtttgatc gcgcctattc tccacagcag gaagtgcgcg aaaagtgcat tgaagctacg
     6961 aggtttgctc gtgtccccgg aggtcaatgg gaaggagcaa cggcggctgg aactaagctt
     7021 gatgagcagt tcgagaagta tcctaagttt gaaatcaata aggtagcaac tgaacttaac
     7081 cgcatcattg cagaataccg caataacaga atcactgtta agtttcgtcc tggtgacaga
     7141 gaggcaagcg aagagttagc caataaatta aatggtctgt tccgtgctga ctacgaagaa
     7201 accgatggcg gtgaggcttg cgataatgca tttgacgacg ctgctactgg tggtttcggt
     7261 tgcttccgtt tgacgtcgat gctggtcaat gaatacgacc ccatggacga tcgtcagcgt
     7321 attgctattg aaccaatata cgacccgtcg cgctctgtgt ggtttgaccc tgacgctaag
     7381 aagtacgaca aatctgacgc gttgtgggcg ttctgcatgt attcgttgtc acctgaaaaa
     7441 tatgaggctg aatacagaaa gaaacctcct gcttctctgg atgtaacgtc tatgaccagt
     7501 tgggaatatg actggtttga tgaagatgtt atttacatag cgaagtatta cgaagttcgt
     7561 aaagagtctg ttgtcgtcat cagttatcga catccaatca ctggagagat tgcaacatac
     7621 gacagtgatc aggttgaaga tattgaagat gaactggcaa tagctggatt tcaggaagtg
     7681 gcaaggcgct cagtgaagcg ccgtcgtgtg tatgtatccg tagtggatgg tgatggtttc
     7741 cttgagaaac ctcgacgtat tcctggtgag catatccccc tcatcccggt ttatggaaaa
     7801 cgctggttca ttgatgacat tgagcgtgtc gaaggacata ttgcaaaagc aatggatcca
     7861 cagcgtttgt ataaccttca ggtatcaatg ctggctgata ctgcagcgca agaccccggt
     7921 cagatcccta tagttggcat ggagcaaatt cgtggacttg agaagcactg ggaggctcgc
     7981 aacaagaaac gcccagcgtt cttgccgttg cgcgaagtga gagataaatc tggcaacatt
     8041 atcgctggag ctaccccggc aggatataca cagcctgcgg ttatgaatca ggcattggct
     8101 gcattactac agcaaaccag tgctgatatt caggaggtta caggcggcag tcaggccatg
     8161 cagcagatgc caagtaatat tgctcaggaa acggttaaca acttgatgaa cagagcagat
     8221 atggcttcgt ttatctatct ggacaatatg gcgaaaagtc ttaaacgcgc tggtgaagta
     8281 tggctgtcaa tggcgcgtga agtgtacggt tcagagcgtg aagtgcgcat cgttaacgaa
     8341 gatggaagtg atgatatcgc tgtcctgagc gcacaggttg ttgacaggca aacaggggct
     8401 gttgttgcgt taaatgacct ttctgtcggt cgatacgatg tgacggttga tgttggacca
     8461 agctacacag cacgacgtga tgcaacggtt tctgtactga caaatgtcct tagctctatg
     8521 cttccaacag acccaatgcg cccggcaatt cagggtatta ttctggacaa tatcgatggc
     8581 gaaggccttg atgacttcaa agagtacaac cgaaaccaac tgctgatatc tggtattgca
     8641 aaaccacgca atgagaaaga gcagcagatt gttcaacagg cgcaaatggc agcacaaagc
     8701 cagccaaatc ctgaaatggt tctcgctcag gcgcaaatgg tagcagcgca ggcagaagcg
     8761 caaaaagcaa ctaacgaaac tgctcaaact caaatcaaag catttactgc ccagcaggat
     8821 gcgatggaga gtcaggcaaa cactgtctat aaactggctc aagccagaaa catcgatgac
     8881 aaagcagtga tggaggcaat acgccttctg aaagatgtcg ccgagtcaca acaacagcaa
     8941 ttccagtcac caccacagtc accggcagac ttaatgccga gttaaccagg agtaatcaat
     9001 ggaaaacgaa ctgatcatcg acggtcaggt tattgacctg tctgaaacac aggaaaatgc
     9061 agaagaaacc atcatccaaa cagagtcaca gcctgagaat gaaagccagg atgacaacgg
     9121 aaaagagatg gcaactgatc ctgaaaaaac cgaagagaca ccagaagatt acgccttgcg
     9181 tattggtgat gaagaaattc agcttaacgc tgacgataat gatcacattg acgggcaacc
     9241 tgcaccgcaa tgggtgaaag atcttcgcaa aggcttcaaa gaaacacaga aagaaaaccg
     9301 tgagttgcga cgccagcttg aggaagcatt agccaagcat gcggaacatc agcaaccaca
     9361 accagacgct attccaccaa aaccgactct tgagtcgtgt gattatgacg aacaggcgtt
     9421 tgaacaggca ttgactgatt ggcatgagaa aaaaggccgt gtcgaacagc agcagcaaca
     9481 aaaactacgt cagcaacagg aataccagca gcgtttccag caaagggtag aagcgcataa
     9541 acaacgggca gccaaacttc ctgtgaaaga ttatcaggaa atggaagcca ttgttcttag
     9601 tgagctacca ccaattcagc aggaaatcat cattcactgt gcagacgaag gctctgaact
     9661 actcgcctat ggcttaggta agagccagca attacgccag cgtgtagccg ctgagacaga
     9721 tccaattcgc gcagcattcc tcttggggca gattagcaaa caggtaagcc ttgctccaaa
     9781 accaaagaaa gccatcaagc cagagccgga agtacgtggt ggcggtgctg atgcgaaaca
     9841 agacgaattc aacaaattat gccccggcgc aaaaatcgaa taaggaaaag ataaatgcct
     9901 aacaatctcg acagtaacgt cagtcaaatc gttctgaaaa aattccttcc gggttttatg
     9961 tcagatttag ttctggcgaa aaccgtagac cgtcagttgc tggcaggtga aatcaactcc
    10021 agcactggcg atagcgttag ctttaaacgt ccgcatcaat tctcatccct ccgtactccc
    10081 actggtgata tttcagggca aaataaaaac aacctgatct caggtaaagc tacggggcgt
    10141 gtaggtaact acatcactgt tgctgttgaa tatcagcaac tggaggaagc gatcaagctt
    10201 aaccagctgg aagaaattct cgcgccggtt cgccagcgaa tcgttaccga ccttgaaaca
    10261 gagcttgctc acttcatgat gaataacggt gcgttgtcac ttggtagccc caatactcca
    10321 atcaccaaat ggtctgatgt tgcgcagacg gcatctttcc tgaaagacct cggcgttaat
    10381 gaaggtgaaa actatgctgt aatggatcca tggtctgcac agcgacttgc tgatgcgcag
    10441 actggtttgc atgcttcaga tcaattggtt cgtactgcat gggagaacgc acagatccca
    10501 accaattttg gcggcattcg cgcactgatg tctaatgggc ttgcctctcg tacgcagggg
    10561 gcatttggcg gaacactgac agtcaaaaca cagccaactg ttacctataa cgcagttaaa
    10621 gactcatacc agttcactgt aacattgacc ggagcgacag ccagcgttac aggttttctg
    10681 aaagctggtg atcaggtcaa attcaccaat acctactggc tgcaacagca gaccaaacag
    10741 gcgttgtata acggagccac accaattagc ttcactgcaa cggttactgc tgatgctaat
    10801 tcagacagca gtggcgatgt gacggttacg ctttctggtg ttccgattta tgacactaca
    10861 aacccgcagt acaactctgt aagtcgtcag gtagaggcag gcgatgccgt atctgtagta
    10921 ggcactgcta gccagacaat gaagccaaac ctgttctata acaagttctt ctgtggactt
    10981 ggatctatcc cactgccgaa actgcacagt attgattctg ctgttgcaac atatgaaggt
    11041 ttctccatcc gcgtacataa atacgcagat ggcgatgcca acgtgcaaaa aatgcgcttc
    11101 gacttactgc ctgcatatgt gtgctttaac ccacacatgg gcggtcagtt cttcggtaat
    11161 ccgtaataac aaggggctta cgcccctttt atgttttaag gaaacaatat ggatcggatg
    11221 agtgtattcc ttgccgcaga taacgaatcc gggcatgtac aggccgttat cgcagaaaaa
    11281 gacttccagt ttttcgaaag gttgggcttt gttgcctcag ttgatgaatt gaaaccgacc
    11341 agtaagcgag gtcgtaaggc ggcggacaat ggcaacagta ctgacaaagg gtgagatcgt
    11401 cctttttgcg cttcgtaagt ttgctattgc ttctaatgca tcgcttactg atgttgagcc
    11461 gcaatcaatt gaagatggtg taaatgatct ggaagatatg atgtccgagt ggatgattaa
    11521 ccccggcgac attggttacg ctttcgcaac tggagatgag cagccattac cagatgatga
    11581 gtcaggtctt ccaagaaaat acaaacacgc agtaggctat cagttattgc tgagaatgct
    11641 atctgattac agccttgaac caactccgca agttctcagt aacgcccaac gctcatatga
    11701 tgccttgatg accgacactc tggttgttcc ttcaatgcga cgacgtggag attttcctgt
    11761 agggcagggt aataaatatg acgtgttcac atctgaccga tattatccag gcgatctccc
    11821 tctgattgat ggcgatatcc caaacgcata ggtgaataaa tgcctattca gcaacttccg
    11881 cttatgaaag gtgtcggcaa agactttaga aacgccgact atatcgacta tctgccagtg
    11941 aatatgttgg ctacacccaa agaaatcctg aacagcagcg gatatcttcg ctcattcccg
    12001 ggcattgcca aacgttctga tgtgaacggc gtatcgcgag gcgtcgagta caacatggca
    12061 cagaatgctg tttatcgcgt gtgtggtggc aaactgtata agggcgaaag tgaggtcggt
    12121 gatgttgccg gaagtggtcg cgtatcaatg gcgcatggtc gaacatcaca ggcggtaggc
    12181 gttaacgggc aactggtcga ataccgctat gatggcacgg ttaaaaccgt ctcaaactgg
    12241 cctgcagaca gcgggtttat gcagtatgag ttaggttctg ttcgcgacat tacgcgttta
    12301 cgtgggcgtt atgcgtggtc aaaagacggc actgattcat ggtttatcac tgaccctgaa
    12361 gacgaatcgc atcctgaccg ctacagcgca caatatcgag ccgagtctca gcctgacggc
    12421 atcatcggca tcggaacatg gcgagacttc atcgtctgct ttggttcatc gacgattgaa
    12481 tatttctccc tgacgggggc aaccaccgtt ggtgccgctt tgtatgtcgc acagccatcg
    12541 ctgatggtgc agaaaggcat tgccgggact tactgcaaaa cgccgttcgc cgattcgtat
    12601 gcgttcatca gcaatccggc aacaggtgcg ccgtctgtgt atatcatcgg ctccggtcag
    12661 gtatcaccaa tcgccagcgc gagcattgag aaaattcttc gctcctacac tgctgatgaa
    12721 ctggctgatg gtgtgatgga atcgctgcga tttgatgcgc atgaactgct gattatccac
    12781 cttccgcgcc acgtcctcgt gtacgacgca tcttcaagcg ccaatggtcc gcaatggtgt
    12841 gtgttgaaaa caggcctgta tgacgatgtg taccgcgcta tcgacttcat ttacgaagga
    12901 aatcagataa cgtgcggcga taagctggaa tcggttaccg ggaaattgca gttcgatatc
    12961 agcagccagt atgggctaca gcaagaacac ctgctgttta ctccgttgtt caaagcggat
    13021 aacgccagat gctttgatct ggaggtggaa tcatcgacgg gtgttgctca gtacgctgac
    13081 cgcctgttcc tctctgcaac cactgacggc ataaattacg ggcgtgagca gatgattgag
    13141 cagaatgaac cgttcgttta cgacaaacgc gttttgtgga agcgagtagg gcgcatcagg
    13201 aaaaatgttg gcttcaaact gcgcgttatc actaagtcgc ctgtcactct gtctggcgct
    13261 cagataagga ttgagtaatg gcggattcga atctcaatgt gccggtaatc attcaggcta
    13321 cacggctcga cacatcagtc cttccacgca atatcttctc gcagtcgtat ctgctttacg
    13381 ttatcgcaca gggtactgat gttggtaatg tggctaacaa ggccaacgag gccggacagg
    13441 gcgcttatga cgcacaagtc aggaacgatg agcaggatgt gattctcgct gaccatgagc
    13501 agcgaatttc tgctgcggaa gcaacgcttg ttaatcatga ggagcgaatc agccaggcag
    13561 aatcaactct tcaggaacat gaaacgcgaa tcgctcagaa tgaaagcgat attgcgtcgc
    13621 ttgataccag agttcagtcg ctggaatcgc aggtttcaga ccatgaatcg cgcattgatg
    13681 ctctggagta tgccactact cgcaagaagt cagaggttgt ttactctggc gtatctgtaa
    13741 ccatcccgac agcgccgacc aaccttgtta gcctgctgaa aacgctcacg ccgtcatcag
    13801 gctcgttggc accattcttc gacaccgtta acaacaagat ggttgtgttc aacgagaaca
    13861 aaaccttgtt cttcaagctg tcgatcgtcg ggacgtggcc cagcggaacc gccaacaggt
    13921 caatgcaact aacattttcc ggctctgttc ctgacacact ggtaagcagt cgcaactcgg
    13981 cgacaacgac cgataacatc ctgttagcta cgttcttcag cgtggataaa gacggctttc
    14041 ttgccacaaa tggcagcacg ttaaccattc agtcaaatgg tgcggcgttt actgccacaa
    14101 ccatcaagat aatcgcggag cagtaatgat tcagttcaaa ccaacgcgaa acatcgacct
    14161 gatagaagca gtcggaaatc accctgacat tattgccgga agcaacaacg gtgatggata
    14221 cgactacaag cctgaatgcc gttactttga ggttaacgtg tacggtcagt ttggcggcat
    14281 tgtttactat caggaaattc agccgcttac attcgattgc cacgccatgt acctgccaga
    14341 ggttcgtgga ttcagcaagg aaatcgggct ggcgttctgg cgatacattc tgactaacac
    14401 caccgttcag tgcgtcacat cgttcgctgc gcgcaaattc cgccacggtc agatgtactg
    14461 cgcaatgatt ggccttaagc gtgtaggaac catcaagaaa tacttcaaag gcgtggatga
    14521 cgtgacgttt tacagcgcca cacgcgaaga actaatcgac ttcctgaatc acgggagata
    14581 gctatgttat atgcatttaa gctgggcaga aaactgcgcg gcgaggaacc ttattgccct
    14641 gaaaaaggcg ggaaaggtgg cagctctgat aaaagcgcaa agtatgcagc agaagctcag
    14701 aagtatgccg cagacctgca aaatcagcag tggcagacga tcatgaaaaa ccttgctccg
    14761 ttcacgccgc ttgcggagca gtatgttaac cagcttcaga atctttccag tttagaaggt
    14821 caggggcagg cacttaatca gtattacaac tctcagcagt ataaagacct tgcaggtcag
    14881 gctcgttacc agagtcttgc tgctgcggag gcgacgggtg gacttggttc gacagccaca
    14941 agcaatcaac tggctacgat cgcgccgaca ctcggtcagt cttggttatc aaatcagatg
    15001 agcaattaca acaatctggc aaacgttggg cttggtgcac tgcaaggtca ggcaaacgcc
    15061 gggcagacgt acgccaacaa catgagcagc attgcacagc aaagcgcagc acttgccgct
    15121 gctaatgcca ataaaccatc aagtcttcag actgcaatta gcggcggcac atctggtgcg
    15181 attgccggtg caggtcttgc cagccttttg ggaacatcaa cgccttgggg cgctggcatt
    15241 ggtgctggta tcggattgct tggctcgttg ttttaagggg taatcatggc tacttggcaa
    15301 ggaacaaacg gcggattgtt ggctggtatc ggcggcgtca actcaaacgc tccgagcgta
    15361 aatgacatcg gcaatacgct tcagcttatc aggcagaaca atgatattga tcgttcaggc
    15421 gctaacaatg ttgggctgac tgctttgcaa ggcctttcag gtattgcagg ggtgtttcag
    15481 caggaaaagc aggctcagcg gcagaaagaa tttcagcagg cgtacgctaa tgcttatgcg
    15541 tctggtgatc gcggtgcttt gcgtcagttg gctactcaat atccagacca gattgaatcc
    15601 gttcgtaaag gcatgggatt cattgatgaa gaccagcgta attctatcgg caccttagcg
    15661 gctggcgcac gccttgcgtc atcgtctcca gaagcaatgc aatcatggct gcaaaacaac
    15721 gccaaggaac tgactcgcgt cggtgttgac cctaacagcg ttgctcagat gtatcagcag
    15781 aacccttcag gatttggtga gtttgttgat caccttggaa tggctgctct tggtccaatt
    15841 gattacttca atgttcagga caagatggct ggtcgtgaaa ttgaccgagg cagactggca
    15901 gagacaatcc gcagcaatca ggctggagaa gcacttcagg cgagagggca aaacctttcc
    15961 tatcagtcag caatgactgg gcacaatatc gcagcacaac gcttggctct ggatcagcaa
    16021 gagttcgggt ttaagatgca gcaagcgcag gaaaaggctc agcagttgat tagcgaagca
    16081 cctaagctgt cagtaaacat ggaaaaaggc atcgagacgg ctgtaaacaa tgctacagca
    16141 tcatcaaact cagccaattc tatgagtgcg cttgctcaac agttcagagc agaaaaacca
    16201 acgacaggtt tgttcggtaa cgcacagaac atgttcgcaa aacttaccgg aagcgataca
    16261 acattgcgtg atttgcgcat tcgccaaaat gcccttgtta acagtcaggt tcttaaattc
    16321 ctacctcccg gcccagcaac ggataaagac gttgagatcg ttcgacaggg tgcgccaact
    16381 gacatggata accctgagac ggtcgcaaga tggcttgatg caatggcaaa ccttgagcga
    16441 cgaaacgcgc agtttaatga gtttaaagcc gagtggatga gcgcgaatgg caaccctgga
    16501 caatcgcgta atggcggtca gatattgggg ttggatgtta aaaaaggtga atcattgggg
    16561 agtgccgtta agcggtatat gtcaatgaat actgacgcag cgccagcaca agattcgaca
    16621 ccttcaggag aaccacggaa tcaggttgga tcatatacct caaaatcagg cattcaattt
    16681 acggtggaat gatgaaagta actgcaaacg gtaagacatt tacctttcct gatggtacga
    16741 gcaccgaaga tattggcacc gccattgatg agtattttgc tggtcaggct gttcagcaac
    16801 aaacagttaa tcaggccaat aatgcaccaa cacgggacga accatcattg atgcaacaag
    16861 ctggcgattg gctcactggt ggtcaaagtg cagggcaaat tgcagaacag gctggtcgtg
    16921 gtctggtaaa cataccattt gacgtattgc agggtggcgc aagtctgatt aatgcaatca
    16981 gtcaggggct tggtgggcca aaagttttgg atgatgttta tcgtccagta gacaaaccga
    17041 cagacccata cgcacaagcc ggtgaaacaa ttggtgggta tttagttcca ggagttggaa
    17101 cggcaggaag catggctatt ggatcgctgg cagaggccgc aaatcagaaa ggcgatttcg
    17161 cacaaaatgc agctaaaaat gccggagtta accttgccgc tcagggtgtt ctttccgcag
    17221 cagcaaaggg aatagggcgt ggaataacgg ctataaaagg tgatattgcg ccagaagtgg
    17281 cgaagaaaat tgccacatca gaatcgatgg gcgtgacacc aatgacatct gatgttatcc
    17341 cgccgaaaaa tgctttcact cgcggcctta ctcaggatgc cgagggggct ttgctcggga
    17401 cgggctcaaa gcgagcagag caatatgcaa cgcgtagtaa gctggtaagc aattattttg
    17461 accgttttgg tgagtacaac cctgatgatg tggtgaaatc tctgaccacc acgttaaggg
    17521 ggcggaagga tgctgctggc gctgttatca atgacgtcac caataaaatg ggtaatgccg
    17581 cagttgatac tacaaatacc atgaatgctc tgaatacagc gatcgcaaga caggaacggc
    17641 ttgggacttc agccaatcaa agcctgctta catccttgcg taacctgcgt gaagaattag
    17701 caaaccctgc aactgatttg gatgttacgt ttgatctctt gcgtcagcac agaacagcat
    17761 ttagatctaa tgttcaggga gatgctatgg tcttccccaa ccaggcaaaa gcagctacca
    17821 atatggtaga gaatgcaatg tcaaaagacc ttcgtaacgc agttgcaaaa aacctcggtg
    17881 cgtcagacgc agcaaaatac cttaaagcaa attccgatta tgcaaacgtt tataataagg
    17941 tgcttaataa aaacattgct aacaagctca acaaggcaag cagtgaagcc agtcctgaac
    18001 ttataaatac tgttgtatta agcagaaaac catctgacgt gaaacgaatc tggagcgcat
    18061 tggatgataa agggaaagat gctatgcgtg cagcttacgt cagcaaaata gcggaaaagg
    18121 ccggtgactc tccagccaag ttcatcactg aagttaataa gctgaaatct cagtcaggtg
    18181 gtgaaattta caacactatt ttttctggaa agcacatgaa agagcttgat tctcttcatg
    18241 aagttctaca gaaaacagca aggtcagaca ccgcaaatgt agtaactcag actgggcaat
    18301 cgcaagccaa caggataagg acgattggcg caactgcgac tcttggcgta tcaatggggc
    18361 ttgaggctgg tttcggtgca atgatgcgct tgtatgagtc caaagcagca aggaatgctc
    18421 tcttacgttt ggcaaacacc aaagcaggaa caccagccta tgaaagagcg ctaaataatg
    18481 ctgcaaatgc gatacgccct atactctcaa gccaaattac agcagaacag caataaaaga
    18541 taagatgtaa ctacctgata ttactgctac tgttgcatgt taccgtgttt ccaaatcctg
    18601 aattgcagtt tgtatatgtg tcaacgcgtg ttgggtaagg ttgagttata acaggctggc
    18661 gcgctttttg ctcgatcgct tgcattgtgt ttacagcctg ataattcaat aaagcctgct
    18721 ggaatgcttg gctttgtgct atttgttggg cttgttcttg gctttgtaat tgaacataaa
    18781 gattctgaag ctcaagtctt gcctgtgcgt cacttatctt gccttcatcg acacctttcc
    18841 cgagcatctt tgcagcaagg acatacagct taggtgttgg tgctgatgcc atgcgtgagt
    18901 cgttcttcac actggcatca aggcaattag ccatatcgct aagcttttga tagcgttgtt
    18961 cgcaacttgc ttgatagtca cttacttttg cgcatccaac cagcagaagc gggataatta
    19021 acagtgattt tttcatatgg ttaactctcc ttagtttttc acaggatatc atgaaggcaa
    19081 tgccatttta gccggaaact agatttctat gtttcctttt tattattgct atacatggtc
    19141 ttaagcgttt caaaaaccat tttcttaacc atatcagatt gttgttctgc catacgctct
    19201 gcatcatcaa tgtaaacgga tgcagagctt tgtttagcca acgattcttc aatcgctgca
    19261 attatctctg agttcagcga cctgttattc atcttcgcgc gctgtttaat tttcgcgtgg
    19321 agttcatgcg gaagtctcaa gtgaaactgc gcctcgtcgt atttgctgta catccttgat
    19381 gcctcaccag ttgggtggaa tggcatcgta acctactgga taaatactca atagtaccat
    19441 ttcggtatgc aatcacatca tggttgcatc atatcattct tctggagcaa tgaaatgtca
    19501 gatatcaccg caaatgttgt ggtcagcatg ccttcgcaac tcttcactat gtctcgctct
    19561 tttaaagccg tagccaatgg aaaaatttat atcggtaaaa ttgacactga cccggtaaat
    19621 ccagaaaacc agattcaggt ttatgtggaa aacgaagacg gttctcacct tcctgtttcg
    19681 caaccaatca tcattaacgc tgctggttac cctgtatata acggacagat tgccaagttc
    19741 gtaactgtgc aaggccattc tatggctgtt tatgatgcat acggtgcgca gcagttctat
    19801 tttccgaatg tgctaaagta tgatccagat cagtttaagg gcaatctcct gagcgaagat
    19861 cactcacttg gcgactctct agtcatgcat aacccacagt ttgtcgatag cacatcgacc
    19921 attctctcaa aaaagctaac tatcgacctg aggacgctta cagactacgg gtttgctgag
    19981 ggaaacaccg gtgctcagaa caaagctgca tttcagaagg ctattgatga tgctacgttg
    20041 ccaactgaga ttgttatacc agagggggcg ttcattgttg accctggtat tacgattaag
    20101 aatactgtca ccatgatacg aggtgctggc gcatatcagt ccagaatttt ctctacgggt
    20161 acagcggcac cgataatcac gcaacaagat ggggtaatta cgttctgcga gttcagggac
    20221 tttggtcttg acggaaatgg ctacgcagct aacggcatca gtcttactga agccaaccac
    20281 attaaaatag aaaatattga cgtggttaat actaataaca acgcaatatt agttaacggt
    20341 tactccattg acatcattgg gtgccggttg tttcaaaacg ccggtaacgg tattaatgtt
    20401 ggtggttact gcaataatat taatataatc aacagccgta tctatggtaa cggagccggt
    20461 ggagttttac ttacgccagc ttacgctgag ggcggtatga gcgtcagagt taatggcaac
    20521 aatatagagc aaaataagtt ctacggactt ttggcttacg gggttaaagg ccttaacctt
    20581 gatgctaact attgggaacg caacggtgag attggttacc cgtatagtgt acctgagtct
    20641 attacagtac gtgcggatat ccaccttatc gcgaacaatt ttacattaat tcctgacctt
    20701 tcaaaaatta atgataccgt atctattcgc gggaatcagc agaccgcgat tggctacgcc
    20761 agcgcgctac ctaaccaaga cggatttatt ttcacgaact atgcgaaaaa cctgacgatt
    20821 gaaaataatc agttattaga cgcatcgaaa gtgaataatt tattagcaat gtaccacaat
    20881 aatctttcgt ccaaagttac tgacaggcta tatcttgcca acaacacagt gaactccatc
    20941 ggatacgttg gtagttatga cccggcgaca caaaaccctg acactgcgca tttaatagat
    21001 attgcgaaca gagagttaac tgctaactat cttgatcgaa atatgttgct ctggactgcg
    21061 gcatctggaa ctacaggaac attgataaaa actcaaaaca tctatgctgg taattattca
    21121 ttcttagtta cgacaggaga tagagtttgg ggacgaacga tagacctcaa caaatcacca
    21181 gaactaaaag ggaaatttgt atggtttggt gcgtgggtca atgaccaggg atcagcgtca
    21241 aaacttatgt ttataattaa tggtgcagga caaacagact ctacagcacc tttagctggt
    21301 aatgggaaat ggagctatgt tagttgtggt gtgtatatat acgaaactga tacagctata
    21361 aatgttggta taagaaatta tggttcagga aatgtactca taaactcacc ttctctatgt
    21421 gcatatggaa tgccatcaaa cgcgttgcaa gttgaaaaaa caacatttta tctttcgtca
    21481 gtacctacat ccggtttctg ggatataggt gagcgtgtta tcaatagcgc acctgcttca
    21541 ggacaaccaa aggcatggac atgcaatatt ccaggtgggc caggaaccta ttctttctta
    21601 tcagagggaa acttttaaaa gacctatgta ttgaaatgat tactttgatc tggtatgttt
    21661 aaaaaaaatc agtaaaacac attttgatat gg
//
LOCUS       DAZKGS010000069        21606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKGS010000069 DAZKGS010000000
VERSION     DAZKGS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..686)
                     /locus_tag="ACHOQY_002606"
                     /pseudo
     CDS             complement(1..686)
                     /locus_tag="ACHOQY_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(864..2513)
                     /gene="actP"
                     /locus_tag="ACHOQY_002607"
     CDS             complement(864..2513)
                     /gene="actP"
                     /locus_tag="ACHOQY_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9678685.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2510..2824)
                     /gene="yjcH"
                     /locus_tag="ACHOQY_002608"
     CDS             complement(2510..2824)
                     /gene="yjcH"
                     /locus_tag="ACHOQY_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9678686.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(3025..4983)
                     /gene="acs"
                     /locus_tag="ACHOQY_002609"
     CDS             complement(3025..4983)
                     /gene="acs"
                     /locus_tag="ACHOQY_002609"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9678687.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            5375..6811
                     /gene="nrfA"
                     /locus_tag="ACHOQY_002610"
     CDS             5375..6811
                     /gene="nrfA"
                     /locus_tag="ACHOQY_002610"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP9678688.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6856..7422
                     /gene="nrfB"
                     /locus_tag="ACHOQY_002611"
     CDS             6856..7422
                     /gene="nrfB"
                     /locus_tag="ACHOQY_002611"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9678689.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            7419..8090
                     /gene="nrfC"
                     /locus_tag="ACHOQY_002612"
     CDS             7419..8090
                     /gene="nrfC"
                     /locus_tag="ACHOQY_002612"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9678690.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            8087..9043
                     /gene="nrfD"
                     /locus_tag="ACHOQY_002613"
     CDS             8087..9043
                     /gene="nrfD"
                     /locus_tag="ACHOQY_002613"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9678691.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            9063..10781
                     /gene="nrfE"
                     /locus_tag="ACHOQY_002614"
     CDS             9063..10781
                     /gene="nrfE"
                     /locus_tag="ACHOQY_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9678692.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10774..11157
                     /gene="nrfF"
                     /locus_tag="ACHOQY_002615"
     CDS             10774..11157
                     /gene="nrfF"
                     /locus_tag="ACHOQY_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9678693.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLRLMALILWRVRAKR"
     gene            11154..11750
                     /gene="nrfG"
                     /locus_tag="ACHOQY_002616"
     CDS             11154..11750
                     /gene="nrfG"
                     /locus_tag="ACHOQY_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9678694.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            12092..13405
                     /gene="gltP"
                     /locus_tag="ACHOQY_002617"
     CDS             12092..13405
                     /gene="gltP"
                     /locus_tag="ACHOQY_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9678695.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13483..14172)
                     /gene="yjcO"
                     /locus_tag="ACHOQY_002618"
     CDS             complement(13483..14172)
                     /gene="yjcO"
                     /locus_tag="ACHOQY_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9678696.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(14266..16413)
                     /gene="fdhF"
                     /locus_tag="ACHOQY_002619"
     CDS             complement(14266..16413)
                     /gene="fdhF"
                     /locus_tag="ACHOQY_002619"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15994..15996),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP9678697.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16611..18077)
                     /gene="mdtP"
                     /locus_tag="ACHOQY_002620"
     CDS             complement(16611..18077)
                     /gene="mdtP"
                     /locus_tag="ACHOQY_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9678698.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(18074..20125)
                     /gene="mdtO"
                     /locus_tag="ACHOQY_002621"
     CDS             complement(18074..20125)
                     /gene="mdtO"
                     /locus_tag="ACHOQY_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9678699.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(20125..21156)
                     /gene="mdtN"
                     /locus_tag="ACHOQY_002622"
     CDS             complement(20125..21156)
                     /gene="mdtN"
                     /locus_tag="ACHOQY_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9678700.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(21175..21450)
                     /gene="ytcA"
                     /locus_tag="ACHOQY_002623"
     CDS             complement(21175..21450)
                     /gene="ytcA"
                     /locus_tag="ACHOQY_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9678701.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4938 a         5643 c         5822 g         5203 t
ORIGIN      
        1 aattgaacat gcatagcgga gcatgcattt agagttccgt cggccatttt tatctgagcg
       61 aaatttgagt catcgagaat acaattatca aaaagtattt tttccattat ggcgtaataa
      121 aaattcgttt tatacatatt acagtcctgg aaaatagcgt tttgtagtca gcaacctgca
      181 aaattcgaat tggataaatc ttcatcttta atcacgatac ctttcaggct aaggtgtgaa
      241 aaatcgtaac caccatttac tgtctgtgga atttcgatag tattattatg gacggcgaca
      301 ttatttataa aatataccac cttaatggat acttcctttt ctttactaag gtaaactctt
      361 ctcgtaattc tatcgtcaca tttattattt gcatataaag ctatatctat aaaatgttta
      421 tgtattttcc tgtccgtttc cttcgtgatt tttcctgatg gtaaactatc ataaattctt
      481 ttcattttct gaatcaaacc tatcactttt tcatgatcat ccatcttgaa aagagagagg
      541 gctttattgt caattaacgt gtttttatga tgtttgctgt aattatatga ttttgttttc
      601 tttgtatgtg atgaacttgt gaaagagtgg ttatcgttaa tctggcaaag agagaaaaac
      661 atattattta aaccattatc gcgcataaaa tatccttata attaacaaga aagaaaaggc
      721 atattctctg cattatcatt ttctacgact gtcaaaaatc gctcattttt taatgagttt
      781 atttgtttaa tattatggga aaaggtgatg catgctggag aggaagagta ttccccggtc
      841 aggcgaccgg ggaaggggag agattaatgt gcgcggcctt gctcaacgcc aaagccggtc
      901 tgggagcgga taaactgtgc acggaacagt tcacgctcgc gcgcaccttc cgctgagtta
      961 tcggttgccg agaagaacca gatgccgagg aatgccacgc tgatagagaa cagcgccggg
     1021 tattcatacg ggaagatggc tttttcgtga ccaaggatct gtacccaaat cgtcgggccg
     1081 aggatcatca gtaccactgc ggtaatcagc cccagccagc cacccatcat cgcgccacga
     1141 gtggtcagtt tcgaccagta catagaaagc agaatgatcg ggaagttaca gctcgctgcg
     1201 atggcaaacg ccaggcctac catgaaggcg atgttctgat tctcaaacag cacgccgagg
     1261 ataatggcaa tcacacctaa aatcagcacg gtgattttcg atacccgcag ttcttcacgt
     1321 tcggtcgcgc cttttttgaa gacgttagcg tacaggtcat gcgaaaccgc tgatgcgccc
     1381 gccagcgtca gaccagcaac caccgcaaga atggtggcaa aagcaaccgc tgaaataaaa
     1441 ccgaggaaca ggttgccgcc cactgcattc gccaggtgaa cggccgccat gttgttacca
     1501 ccaatcagat ggcccgccgc gtctttatat tccggattcg caccaaccag catgatcgcg
     1561 ccgaagccga taataaaggt cagaatatag aagtagccca taaacccggt ggcgtagaac
     1621 acgctcttac gtgcttcgcg ggcatcgctg actgtaaaga agcgcatcag aatgtgcggc
     1681 aagcccgccg taccaaacat cagtcccaga cccagagaga gcgcggagat cggatctttc
     1741 accagcccgc ccggcttcat gatgtcgaca cctttcgggt gtaccgccat cgcttcactg
     1801 aacagattgt tgaagctaaa gccgacgtgt ttcatcacca taaaggccat aaagctggca
     1861 ccgaacagca acagcacggc tttgataatc tgtacccaag tggtcgccag catgccgccg
     1921 aacaggacgt acatcatcat cagcacgccg accagcacca ccgcaatgtg atagttaagg
     1981 ccaaacagca gctcgatcag tttacctgcg cccaccatct gggcgataag gtaaagcgcc
     2041 accaccacca gagaaccaca ggccgaaaga atacgaatcg gcccttgttt cagacggtaa
     2101 gaggccacat cggcaaaggt gtagcgcccc aggttacgca gacgttcggc aatcaggaac
     2161 aaaatgatcg gccagcccac caggaagccc agcgagtaaa ttaagccgtc atagccggag
     2221 gtaaacacca gcgcggagat ccccaagaat gaggcggcgg acatatagtc cccggcaatc
     2281 gccagcccgt tctggaagcc agtgatattg ccgcctgcgg tgtagtagtc gctacgagaa
     2341 cgtacgcgtt ttgatgccca gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac
     2401 ataataatcg cctgccagtt cgttggctgg cgctctacgg ccccgctaat agcatccgcg
     2461 gcgttagctg cgaaagggag tgtggcggca agcgccgtca gaactctttt catgatgctt
     2521 gtacctcatg caggacttca ttattaagac ggtcgaattc gccgttcgcc cgccagatgt
     2581 agataccggt gagaacaaag gagatcacaa tcactccaac accaattgga ataccgcgtg
     2641 tgacgctggt gttcggattc agcggcgtgc ccagccagcc gggcgcgaag gcgatcagta
     2701 aaataaagcc gatataaact gccagcataa taatcgacag gatggtggca aaccgttgcc
     2761 gtttttcgac taactccctg aaatgcgcat tgtcttctat ccgctgataa atcgtaccat
     2821 tcatcacaga ttctccagag gtaatgtagg gattgtttta attcccgctc ccttatggga
     2881 gaaggttaac gctcgggtaa cccttgccga atgtaggccg gataaggcgt tatacgccgc
     2941 atccggcaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac aaaccgttac
     3001 cgactcgcat ccggcaattg tgggttacga tggcatcgcg atagcctgct tctcttcaag
     3061 cagcttctcg actacgccag gatcggcaag cgtcgaggta tcgcccaggt tgctggtatc
     3121 gcccgccgca attttgcgca gaatacggcg cataattttg ccggagcggg ttttaggcag
     3181 ggagtcggtc cagtgcagca cgtctggcgt cgccagcggg ccaatctctt tacgcaccca
     3241 gttgcggact tctgcgtaca gttctggtga cggttcctcc ccgtgattaa gcgtgacgta
     3301 ggcgtagatc gcctgacctt taatattgtg cggaatacct actacggcgg cttcggcaat
     3361 cttcggatgc gccaccagcg ccgactcaat ctctgccgtc cccagacggt gaccggagac
     3421 gttcagcacg tcgtccacac gcccggtgat ccagtaatag ccatcttcat cgcgacgcgc
     3481 gccgtcgccg ctgaaataca tatttttgaa ggtggagaag taggtctgtt caaaacgttc
     3541 gtgatcgcca aacagcgtac gcgcctgacc cggccaggag tcggtgatca ccaggctgcc
     3601 ttcggtagcg ccttccagcg ggttaccttc gttatcgacc agcgccggtt gcacgccgaa
     3661 gaacggacgt gttgccgaac cggctttcag ctcggtagcg ccaggcagcg gcgtgatcat
     3721 gaaaccgcca gtttcggtct gccaccaggt atcgaccacc ggacatttct cgttgccgat
     3781 ttttttccag taccactccc acgcttccgg gttaattggc tcgcccacgg aaccgagaat
     3841 gcgcagcgac gaacggtcgg taccctcgat cgctttatcg ccttccgcca tcagcgcgcg
     3901 gatcgccgtg ggtgcggtat agagaatatt gacctgatgc ttgtccacca cctgcgccat
     3961 acgggcaggc gtcggccagt tgggtacgcc ttcaaacatc agcgtggtcg caccgcaggc
     4021 cagcgggccg tacagcaagt aactgtgtcc ggtcacccag cccacatcgg cggtgcacca
     4081 gtagatatca cccggatgat aatcaaagac atatttaaag gtcagcgccg cgtacaccag
     4141 ataaccgccg gtagtatgca gcacaccttt tggcttaccg gtagaaccgg aggtgtagag
     4201 aataaacagc ggatcttcgg cgttcatctc ttccgcctgg tgctgatcgc tcgcttgctc
     4261 aaccaggtcg tgccaccaca ggtcgcgccc ttcctgccag tcaattttcc cgccagtacg
     4321 cttcagtacc accacatgct ctacgctggt gacgttcggg tttttcagcg cgtcatcaac
     4381 gtttttcttc agcggaatac tgcgcccggc acgcacacct tcgtcggaag tgatcaccag
     4441 tcgtgagttg gaatcaataa tgcgcccggc aacggcttcc ggcgagaagc cgccgaaaat
     4501 caccgaatgc accgcgccaa tgcgggcgca ggccagcatc gcaaccgcgg cttccggcac
     4561 catcggcata taaatcgcca ccacatcacc ttttttaatg cccagctcga gcagggtatt
     4621 ggcgaagcgg cacacgtcgc ggtgcagctc tttatagctg atatgtttgc tctggctggc
     4681 gtcgtcgcct tcccagatga tggcggtgcg atcgccgttt tcttgcagat ggcggtcaag
     4741 gcagtttgcc gccagattca gcgtgccgtc ctcgtaccat ttaatggaca cattaccggg
     4801 ggcaaaggag gtatttttca ccttctggta cggtttgatc cagtcgagaa tttttccctg
     4861 ttcgccccag aaggtatcag gtacgttaat agattgttga tacatcgcct cgtactgctg
     4921 agggtttatc aggcaacggt ctgcgatgtt ggcaggaatg gtgtgtttgt gaatttggct
     4981 catgcttttg ttctccttgt aggatgttaa taatatgtgg cataagcgtt aaatgtaggg
     5041 gtattggcag ttttgtttag tatttgggcg acagatcacg caaaagtaga attgtgcaaa
     5101 taaacggcag ggtaattttt gaaggtcacc aacaaaagtt gattaattct ttgaggaaca
     5161 tgcagttatg catgctgttg aaaaagagga agatactgac taactctaaa gtggtatttt
     5221 acatgcactt acaattgatt aaagacaaca ttcacagtgt ggttatatgt tacacatagg
     5281 ggcgagcaat gtcatgacag tgtaggtgcg gttactgtcg tgaaaacaat aaaaaccgcc
     5341 attgcaacaa tggcgcaatt cggatgaagc ccctatgaca aggataaaaa taaacgcacg
     5401 ccgtatcttc agcttattga ttcctttttt ctttttcact tctgttcacg ctgaacaaac
     5461 ggcagctccc gcaaaacctg taactgtgga agcgaagaat gaaacctttg ccccgcagca
     5521 tcccgatcaa tatctctcct ggaaagccac ctcggaacag tcagagcgtg ttgacgccct
     5581 ggcggaagat ccacggctgg tgatcctgtg ggcggggtat cccttctcgc gcgattacaa
     5641 caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt gaaaccctgc gtaccggtgc
     5701 gccgaaaaac gctgaagatg gtccgctacc gatggcgtgc tggagttgta aaagcccgga
     5761 tgtggcgcgt ctgatccaga aagacggcga agatggctac ttccacggta agtgggcgcg
     5821 cggcggcccg gaaatcgtca acaacttagg ttgtgccgac tgccataaca ccgcctcacc
     5881 agagttcgcc aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg ctcgcgcgat
     5941 ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat cgatggtttg
     6001 cggtcagtgc catgtggagt attacttcga cggcaaaaac aaagcggtta aattcccgtg
     6061 ggatgacggc atgaaagtcg aaaatatgga gcagtattac gacaaaattg ccttctctga
     6121 ctggactaac tccctgtcga aaacgccaat gctgaaagcg cagcacccgg aatatgaaac
     6181 ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc atatgccaaa
     6241 agtgcagaac gccgaaggca aactctacac cgaccataaa attggtaatc cgtttgataa
     6301 cttcgcccag acttgtgcga actgccatac ccaggacaaa gctgctttgc aaaaagtggt
     6361 cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag gttgaagatc aactggttca
     6421 cgctcacttc gaagcgaaag cagcgctgga tgcaggcgcg acggaagccg aaatgaagcc
     6481 gattcaggac gatatccgtc atgcccagtg gcgttgggat ctggcgatcg cttcccacgg
     6541 tattcatatg cacgcaccgg aagaaggtct acggatgctc ggtacggcga tggataaagc
     6601 ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca cccatgaaat
     6661 ccagatcccg gatatctcga ccaaagagaa agcccagcag gccattggcc tgaacatgga
     6721 acaaatcaag gccgagaagc aggacttcat caaaacggtg atcccgcagt gggaagaaca
     6781 ggcacgtaaa aatggtctgt taagccaata accccgttcc gcctcgcaag gggcggaaaa
     6841 cacaatggag tgaatatgag cgtattacgt tcgttattaa ctgccggggt gctggcgtcg
     6901 ggcttgttgt ggagcctgaa cggaattacc gctacccctg cggcgcaggc atctgatgat
     6961 cgctacgaag ttacccagca gcgtaacccg gatgccgcct gtctggactg tcataaacca
     7021 gataccgaag gtatgcatgg aaaacatgct tccgttatca acccgaataa caaactgccg
     7081 gtcacctgca ccaactgtca cggccagcca tcaccgcaac accgcgaagg ggtgaaagat
     7141 gtgatgcgct ttaacgagcc gatgtacaag gttggagagc agaacagcgt ctgtatgtcc
     7201 tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca cgtaaccaaa
     7261 gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag atacgatgca gacgttaagt
     7321 gacaaaggac ggattaagat ttgcgtcgat tgccacagcg atcagcgcac caatccgaac
     7381 tttaacccgg cgtctgttcc gttgcttaag gagcagccat gacctggtct cgtcgccagt
     7441 ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc gtggtggcga
     7501 aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg ttatgcatcg
     7561 gctgtaccgc ctgtatggat gcttgtcggg aagtgaacaa ggtgccggaa ggcgtctcgc
     7621 gcctgacgat tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa tatcgcttct
     7681 tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc ccgaccggtg
     7741 cgtcttttcg tgatgccgct agcgggattg tcgatgttaa tcctgatctc tgcgtcggtt
     7801 gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg gtcacgaaga
     7861 cggcggacaa atgcgatttc tgccgtaaaa ccaatttgca ggccggtaag ctgcccgcgt
     7921 gcgttgaggc ttgcccgacg aaggcgctga cgtttggcaa tctggacgat cctaacagtg
     7981 agatttcgca actgctgcgc cagaagccta cttaccgcta caagctggcg ctgggaacca
     8041 aaccgaagct gtaccgcgta ccgtttaaat acggggaggt gagccaatga cgcagacttc
     8101 cgcatttcat tttgaatcgc tggtgtggga ctggccgatt gccatctacc tgtttttgat
     8161 tggtatttct gccggtctgg tgacgctggc cgtgctgtta cgtcgcttct acccgcaggc
     8221 gggcggtgca gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg gcgcggtgat
     8281 cctcggtctg ttgatcctcg tcttccacct gacaagaccg tggaccttct ggaagctgat
     8341 gttccactac agttttacct cggtgatgtc gatgggggtg atgctgtttc agctctacat
     8401 ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg ccctgcaaca
     8461 acgctggttg ccgaagctgg ggatcgtgca aaaggttctg agcctgctga cgcccgttca
     8521 tcgcggactg gaaacattga tgctggtgtt ggcggtgttg ttgggggctt ataccggctt
     8581 tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc cggtgctgtt
     8641 cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg cgatacgcca
     8701 acgcagtaac ccgcattcca cggaagcgca gtttgtacac cgtatggaaa tccccgtggt
     8761 atggggtgaa atcttcctgc tggtggcatt ttttgtcggt ctggcgctgg gcgatgacgg
     8821 taaagtgcgt gcgctggtgg cggcattagg tggcggtttc tggacgtggt ggttctggct
     8881 tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc aaaccgtggg tcaatcgcag
     8941 ttccggcatt cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg tgttgatgct
     9001 gcgctttttc attctctacg ccggacagtt gacggtggcg taagccagaa aagaggtggt
     9061 ttctggacgt attccttcct gaagtcggtt ttctggcgtt gttgttaagt ctcggggtca
     9121 acgtgttgac cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct gccatgatgc
     9181 gactcacttg catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc tttggcgtac
     9241 tgacgtattg ttttctcatc agcgatttct cggtcattta tgtcgcgcaa catagctata
     9301 gcctgctgtc gtgggaactc aagctggcag cggtgtgggg cggccatgaa ggttcgctgc
     9361 tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat tatcggcagc
     9421 aaaccgatcc gctatttccg ctgacgttag ccgttttatc tctcatgctc gccgcactgc
     9481 tactgtttgt ggtgctgtgg tccgatccct tcgtgcggat atttccacca gcaatcgaag
     9541 gccgcgatct caatccgatg ctgcaacatc ccggtcttat ctttcatcca ccgctgcttt
     9601 accttggcta tggcggtttg atggtagcgg cgagcgtggc gctggcgagt ttactgcgcg
     9661 gcgagtttga tggtgcctgc gccagaattt gttggcgctg ggcactacct ggctggagcg
     9721 cattaacggc ggggatcatc ctcggttcct ggtgggccta ttgcgaactg ggctggggcg
     9781 gctggtggtt ctgggatccg gtggaaaacg cctctttatt accctggctt tctgccactg
     9841 cgctgctgca cagtttgtcc ctgacacgcc agcgggagat tttccgccac tggtcgctgt
     9901 tgctggcgat agtaactctg atgctgtcgc tactgggtac cttaattgtc cgttctggca
     9961 ttctggtttc ggttcatgcg ttcgcactgg ataacgtccg cgccgtgccg ttgttcagcc
    10021 tgtttgcact gattagcctt gcgtctctgg ctctgtatgg ctggcgagcg cgggacggtg
    10081 gtgcggtggt gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct acgctgttgc
    10141 tgttttgcgc agtgctactg atcgtgctgg tgggaacgct ttatccgatg atttacggcc
    10201 tgctgggctg gggacgcctc tccgttggcg cgccgtattt taaccgcgcg acgttaccgt
    10261 ttggcctgtt gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc aaacgcgtgc
    10321 agcttccggc gctggtagct catgcgggcg tgctgttatt tgccgcgggg atcgtggttt
    10381 ccagcgtcag ccgtcaggag atcagcctga atttacagcc gggtcagcag gtgacgctgg
    10441 caggatacac cttccgtttt gagcgcctcg atctgcaagc caaaggcaat tacaccagcg
    10501 aaaaagcgat agtggcactg tttgaccatc agcaacgcat tggtgaactg atgccggagc
    10561 ggcgttttta cgaagcacgt cgtcagcaaa tgatggaacc gtcaattcgc tggaacggca
    10621 tccatgactg gtatgcggtc atgggtgaaa aaaccggagc ggatcgttac gcttttcgct
    10681 tgtatgtaca aagcggtgtg cgctggatct gggggggagg attgttgatg attgcgggcg
    10741 cattgttaag cggatggcgg gggaggaagc gcgatgaata aaggacttct cacgctactg
    10801 ctgttattta cctgttttgc gcgcgctcag gtcgtagaca cctggcaatt cgccaatccg
    10861 caacaacagc aacaggcgtt aaatattgcc agccagttac gttgtccgca gtgccagaat
    10921 caaaacttac tggaatccaa cgcgccggtg gcagtcagta tgcgccatca ggtttacagc
    10981 atggtggcgg aggggaagaa cgaagtcgaa atcattggct ggatgaccga acgctacgga
    11041 gattttgttc gctataaccc accgttaacg ggtcagacgc tggtgttgtg ggcgctgcca
    11101 gtggtgttgt tacggctgat ggcactgatc ctctggcgag tgagggcgaa gcgatgaaag
    11161 agcccaaaat accggtgaaa atgctgacaa cactcacgat tttgatggta tttctctgtg
    11221 tcggcagtta tctgttaagt ccaaaatggc aggcggtaca tgcggaatat cagcgtcagc
    11281 gcgatccgct acatcagttt gccagccagc aaaccccgga agcgcagctt caggcattgc
    11341 aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg ggcgttactg ggcgagtact
    11401 atctgtggca aaacgattac agcaattcgc tgctggcgta ccgtcaggcg ttgcaactac
    11461 gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt gctttattac caggccagcc
    11521 agcatatgac cgcccagact cgcgcaatga tcgacaaagc cctcgcgctg gactctaacg
    11581 aaatcaccgc tctgatgctg ctggcttccg atgcgtttat gcaggcgaat tacgcgcaag
    11641 ccatcgaact atggcaaaaa gtgatggatc tcaactcacc ccggatcaac cggacacagc
    11701 tggtcgagtc gattaatatg gcgaaattgt tgcagcggag atcagattaa tcatctggtg
    11761 atgaagatta tgttattgag ttgtataaaa aataatcgaa tgaacatatg ccaaaaataa
    11821 tcaccaatca gtattattgc agattaaaca aataaaaatc tttccataac aaatggttat
    11881 tcattaatcc tgccataaac tcgcgttatt atgcattaat gcagcgaaaa gctctgttgt
    11941 taaagggttg cgcaacatac cgcgcaaatg atactgatca taagcgttaa aaaaatctac
    12001 aaaccaacgc aacacaattc atgccctggc agtatgtcac gttctcgcgt ttctgaacgg
    12061 ggaacggcgc tccattgagg aagttattct gatgaaaagt ataaaattca gcctggcctg
    12121 gcagattctg tttgctatgg tgctgggcat tctcctggga agctacctgc actaccatag
    12181 cgacagccgc gactggctgg tcgtcaattt gctctctccg gcgggtgata tcttcatcca
    12241 tctgatcaaa atgattgttg tgccgattgt gatctccacg ctggtggtgg gtatcgcggg
    12301 tgttggtgat gccaaacagc tcgggcgtat tggcgcgaaa accattattt acttcgaggt
    12361 gatcaccacc gtcgccatca ttttggggat cactctggcg aacgtcttcc agcccggtgc
    12421 cggggtggat atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga gcactacgga
    12481 agcggtacaa agcagttccc acggcattat gggcacgatt ttgtcgctgg tgccgacgaa
    12541 cattgtggcg tcgatggcga aaggcgaaat gctgccgatc atcttcttct cggtgctgtt
    12601 tggtctgggg ctttcctccc tgcccgcgac gcatcgtgaa ccgctggtga ccgtgttccg
    12661 ctccatctct gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg caccggtggg
    12721 tgtgtttgcg ctgattgcgg tgacggtggc taactttggt ttctcttctc tgtggccact
    12781 ggcgaaactg gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg tagtgctggg
    12841 aattgtggcg cgcctgtgcg ggttaagcgt ctggatcctg attcgtattc tgaaagatga
    12901 gctgattctg gcgtactcca ctgccagctc tgaaagcgta ctgccgcgaa ttattgagaa
    12961 gatggaagcc tacggagcac cggcgtcgat caccagcttc gtggtgccga ccggttactc
    13021 ttttaacctt gatggttcga cgctgtatca aagtattgcc gctattttca tcgctcagct
    13081 gtatggcatt gacctgtcca tctggcagga aatcattctg gtgctgacgc tgatggtgac
    13141 ctcgaaaggg attgctggcg tgccgggcgt gtcgtttgtg gtgctgctgg caacgctggg
    13201 tagcgtaggt attccgctgg aaggcctggc gtttattgct ggtgttgacc gtatcctcga
    13261 catggcgcgt actgcgctga acgtggtggg taatgcgctg gcggtgctgg tgattgccaa
    13321 gtgggaacac aaatttgacc gcaaaaaggc gctggcttat gagcgtgaag tgctgggcaa
    13381 atttgataaa actgcggatc aataattgaa gattgccggg gttatccacc cggcaatgtg
    13441 tggatgcctg atgcgacgct ggtgcgtctt atcaggccta ccttaaccgt tggttaattt
    13501 ttcaaactct tcacacccgg tatcaaaccc ttccatacag ctcaggttca accagtgcag
    13561 cgccttttgt ttgttcttct cgataaaacc ttcctcaccg tttaagaaca tcattcccgc
    13621 ccagtactcg gaataaccgg ttcgggaaat tgcagagctg cgtttgaaat accaggttgc
    13681 cttgtcatcg tcggccttaa tgcccacgcc gttggcgtaa atcaaaccaa gcagcatttg
    13741 ggcatcgacg gcggagtcat tctccagatc ttccgaggcg ttttctaaca gcgaaatcgc
    13801 ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca gcgttacttc
    13861 accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt gtggatagtc
    13921 cagactgacc ggattggtga ttttgatctg cgccagcagc gcgcaggcat cagcatcacc
    13981 gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg agctaaacca
    14041 gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct ttaaatactg
    14101 gctgccaggc tcggagtcgt tcgcgtgggc aaagaatgtc aaaaacaaca tcaatgcgat
    14161 aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa gagttcgaat
    14221 ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg ccagtgccgc
    14281 ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct cggcggcgcg
    14341 ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg ttttcgtaat
    14401 cgggcttaag ttttcggtaa ccagctcgtt acaggcacca atccaccact ggtaggtcat
    14461 gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa ttttgccttt
    14521 acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg cgtcttcggt
    14581 attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac agttaccggt
    14641 catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca tcgggtactc
    14701 gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgtg ccagaccgtt
    14761 cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat ctgaagtatc
    14821 gcggcaaggc cactgaatga agcccagttc gcccattttc tcgtaagtcg caccgtagaa
    14881 atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt agtgcatcgg
    14941 ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca gatcccattt
    15001 cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa acacgccttc
    15061 atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg ttttggtcat
    15121 aaagatgtcc tgaacgataa ccagttccag atcttcaaag gctttacgta ctgccgacag
    15181 ctccgcgtca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca cttcgccatg
    15241 cgctgcgcgg tgcggcagct cgctgatgcg ataaccggta tgagcaggca ggctttccac
    15301 gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact ggtatcccgg
    15361 atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct gaccacgaac
    15421 cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga ggctggtcag
    15481 agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc acaggatggc
    15541 ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg cgctgacgcc
    15601 ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat actcttcaaa
    15661 gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa taatgacatg
    15721 gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt gcatgtcagc
    15781 aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc cgttacgttt
    15841 agcgttaatt acgtgattcg ccacgattgg gtgggaatcc gccgggttgt acccgaaaac
    15901 gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc cattaccgac
    15961 cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc agtcaacgtt
    16021 attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt cgttacccgt
    16081 accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt tgatggcgct
    16141 caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga gtttgccgcc
    16201 acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg tatcgttaat
    16261 gaaatcccag ccataataac ccttcagaca cagggtaccc tggttggttt tcccctgcgc
    16321 tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc aacctgatgc
    16381 gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta atcaaatcac
    16441 gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt ggggtcattc
    16501 cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg tcgtcagttt
    16561 tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt ttattttttc
    16621 tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg aatgctttgg
    16681 atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagtac tggcaaccgg
    16741 gcttcagtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc ggcagcgcgc
    16801 tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgctcgtc gttgagcgtt
    16861 tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga ctggttgtaa
    16921 cgttcaatca tcatgttgct ggcggcgcgc gtgccttcga gattggcatt caaccgtcca
    16981 ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact ggtttttttg
    17041 aataaggtat ccaggtggat ggcatcaagg ccgaaaaacg ctttgatatc aaagctcggg
    17101 tagaacagcg cccgcgcgga atccacctga tctaatgacg cctgaacata ccagcgcatg
    17161 gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg aatgccggtc
    17221 tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc cgcgccaatc
    17281 aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc aatttgttta
    17341 tcgaccgcca gaatctgtgc ccgcgcgccc tggaaaggca cttgtgcttc cagaccgtgc
    17401 gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt ttgttctaac
    17461 agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctac gcccgtggtc
    17521 agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc aatggcggcg
    17581 gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa caaagctact
    17641 gtggcttccg tatagtacgg cccgtccata cccagtgcgg gcgcatccat cgcataaggg
    17701 cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc tgcgacctgt
    17761 aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc ccgcagtttc
    17821 gcttcggcga gggtgtgtga accacttagc gtccgttgga gcagcgcatc cagctgcggg
    17881 tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag atgaatatcg
    17941 tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc cttacgtacc
    18001 agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag cagacgtgaa
    18061 agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc gttaatgccg
    18121 gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc tgttttaata
    18181 aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga ggagaacggc
    18241 tgagtgcggt tgccagaccg gcagcgtatt tctccagggc gtcggcaaac tgagctgctc
    18301 tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca tcccaggcgt
    18361 gaagaatatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt aaggcgcgtt
    18421 cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca caggcattaa
    18481 acgccgcatg cagaccgata cgaatttgca gataagtgcg cagagccgtg acttcctgct
    18541 ggcgtgggat ccgcattact ttacttaaca tacccagcgt gccagccagt ttttgcggca
    18601 gtgtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac accacggtac
    18661 caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact gggccaaaaa
    18721 cgttttcgag cgtggcgagc gcgaaggtga ccaccatctg tgtgccgata taagaagagc
    18781 gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc acaaacagca
    18841 attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc gccagaatcg
    18901 cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa ccgacatttg
    18961 ggttagcgac gatcacgcat gtcagcatac aggtgtgaat gccttcccag tccacgccgc
    19021 tgtaaaaggt gtaacagatc aaacaggcga gcagcgtttt taccgcgtag cgcatatagt
    19081 ctggattggt aaaagcatcg gcggccattg atggcggttt ggctgcgggc gttggcggcg
    19141 tattcgggtc catctgaccc agttgtaaca acgtctggca gatattctcc aggttacatt
    19201 cccgtgccgc catcgcttca ctttcactga tccgccagtc gctttgccag cactgacctt
    19261 cagcaacggc atgctgcagc ttattgattt ctgaggctaa tttttgccga aattcaataa
    19321 ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag taaatgtagg
    19381 ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg gcatcgtccg
    19441 cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc cgcgtttcgg
    19501 gtagcggtgc gaggctgtcc gtcaggtggc taatggcatc atcaagccga tcattaagcg
    19561 cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt aacgtcatca
    19621 gcaaggttgg ataaaggcca acaacgatac accacagcgt taagcgcacg accacttccg
    19681 gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg actgcgaaaa
    19741 agaccagtcc cagccgatgg gtgcgcatca aaaacataca gcccatcagg atcggcccgg
    19801 cgatgaacaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc
    19861 cgatctccag catcgtggcg accacaaata agatcgcgac aaatttggtg taaaacgcgt
    19921 tcgactgaat accgtaaaac agcactgcca gcgataacgc cacgaaaggg atctcaaagg
    19981 tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacggtc tgcggcacgc
    20041 ggcctggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta
    20101 atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc
    20161 gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc
    20221 aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca ccaggccgcc
    20281 gtcatccggt agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct
    20341 gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc
    20401 gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa tagggcgcat
    20461 ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat caaacggcgc
    20521 gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt cgaccgccgc
    20581 acgctgggca actaatgcat ccacgccgct gacggcgctg gcggctgact gcgcctgcaa
    20641 caatacagca ttaagatcgg cttctgcagc gcgctgcgcg gtcctcgcac ggtcaacgtc
    20701 ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc
    20761 ctgtttcgcg gcggcacggg ctttttctac cgtggcgtta accgagtcgg caccaaactg
    20821 ttgcgcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc
    20881 ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca gcaaatcgcc
    20941 ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcgaccgc tgacttccgg
    21001 caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac
    21061 gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa
    21121 tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa
    21181 aaatgccagc cagaacagca tggcgtagag agcaaaaagg gcggtataaa taattccgac
    21241 aaatgccaga ttgatatttg cccgctgaat aactcgcctc gtgatgagca tcaaaataag
    21301 gctggcaatg gcgcagaaaa accagctggg ataataagca ccgatcaccg ggattgccgg
    21361 agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt ttttgagttg
    21421 catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtctgc tgcgaacaat
    21481 atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc tcgatgaata
    21541 aaaatagctt tattcagcca ccgcattcat aacttatata ttttatttat taattggctg
    21601 tatata
//
LOCUS       DAZKGS010000070        21307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKGS010000070 DAZKGS010000000
VERSION     DAZKGS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..542
                     /gene="lpxT"
                     /locus_tag="ACHOQY_002624"
     CDS             <1..542
                     /gene="lpxT"
                     /locus_tag="ACHOQY_002624"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9678702.1"
                     /translation="TNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            954..1520
                     /gene="mepS"
                     /locus_tag="ACHOQY_002625"
     CDS             954..1520
                     /gene="mepS"
                     /locus_tag="ACHOQY_002625"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9678703.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1701..3257
                     /gene="pdeN"
                     /locus_tag="ACHOQY_002626"
     CDS             1701..3257
                     /gene="pdeN"
                     /locus_tag="ACHOQY_002626"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9678704.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            3339..5153
                     /gene="yejA"
                     /locus_tag="ACHOQY_002627"
     CDS             3339..5153
                     /gene="yejA"
                     /locus_tag="ACHOQY_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9678705.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            5154..6248
                     /gene="yejB"
                     /locus_tag="ACHOQY_002628"
     CDS             5154..6248
                     /gene="yejB"
                     /locus_tag="ACHOQY_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9678706.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            6248..7273
                     /gene="yejE"
                     /locus_tag="ACHOQY_002629"
     CDS             6248..7273
                     /gene="yejE"
                     /locus_tag="ACHOQY_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9678707.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            7275..8864
                     /gene="yejF"
                     /locus_tag="ACHOQY_002630"
     CDS             7275..8864
                     /gene="yejF"
                     /locus_tag="ACHOQY_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9678708.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8868..9212)
                     /gene="yejG"
                     /locus_tag="ACHOQY_002631"
     CDS             complement(8868..9212)
                     /gene="yejG"
                     /locus_tag="ACHOQY_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9678709.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(9545..10735)
                     /gene="bcr"
                     /locus_tag="ACHOQY_002632"
     CDS             complement(9545..10735)
                     /gene="bcr"
                     /locus_tag="ACHOQY_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9678710.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(10763..11458)
                     /gene="rsuA"
                     /locus_tag="ACHOQY_002633"
     CDS             complement(10763..11458)
                     /gene="rsuA"
                     /locus_tag="ACHOQY_002633"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9678711.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            11607..13367
                     /gene="radD"
                     /locus_tag="ACHOQY_002634"
     CDS             11607..13367
                     /gene="radD"
                     /locus_tag="ACHOQY_002634"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9678712.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            13492..13776
                     /gene="rplY"
                     /locus_tag="ACHOQY_002635"
     CDS             13492..13776
                     /gene="rplY"
                     /locus_tag="ACHOQY_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9678713.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13915..14922)
                     /gene="yejK"
                     /locus_tag="ACHOQY_002636"
     CDS             complement(13915..14922)
                     /gene="yejK"
                     /locus_tag="ACHOQY_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9678714.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            15104..15331
                     /gene="yejL"
                     /locus_tag="ACHOQY_002637"
     CDS             15104..15331
                     /gene="yejL"
                     /locus_tag="ACHOQY_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9678715.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            15351..17111
                     /gene="yejM"
                     /locus_tag="ACHOQY_002638"
     CDS             15351..17111
                     /gene="yejM"
                     /locus_tag="ACHOQY_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9678716.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKQDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            17186..17262
                     /locus_tag="ACHOQY_002639"
     tRNA            17186..17262
                     /locus_tag="ACHOQY_002639"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17220..17222,aa:Pro,seq:ggg)
     gene            17481..18722
                     /locus_tag="ACHOQY_002640"
     CDS             17481..18722
                     /locus_tag="ACHOQY_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9678717.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            18821..19051
                     /locus_tag="ACHOQY_002641"
     CDS             18821..19051
                     /locus_tag="ACHOQY_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678718.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            19219..19425
                     /locus_tag="ACHOQY_002642"
     CDS             19219..19425
                     /locus_tag="ACHOQY_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9678719.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            19440..19916
                     /locus_tag="ACHOQY_002643"
                     /pseudo
     CDS             19440..19916
                     /locus_tag="ACHOQY_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            20109..20447
                     /locus_tag="ACHOQY_002644"
                     /pseudo
     CDS             20109..20447
                     /locus_tag="ACHOQY_002644"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            20478..20669
                     /locus_tag="ACHOQY_002645"
     CDS             20478..20669
                     /locus_tag="ACHOQY_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9678720.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            20659..20880
                     /locus_tag="ACHOQY_002646"
     CDS             20659..20880
                     /locus_tag="ACHOQY_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678721.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            20873..21094
                     /locus_tag="ACHOQY_002647"
     CDS             20873..21094
                     /locus_tag="ACHOQY_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678722.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            21096..>21307
                     /locus_tag="ACHOQY_002648"
     CDS             21096..>21307
                     /locus_tag="ACHOQY_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678723.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRY"
BASE COUNT         5322 a         5403 c         5298 g         5284 t
ORIGIN      
        1 tgaccaacaa tcgcgctttc gacggttgtt ctctgctggc gatgggaatg ttgatgctga
       61 gtttctggct gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca
      121 tgctattaac tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg
      181 ccagcccaac attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca
      241 cgaaagatgc ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt
      301 cggcattcat gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg
      361 tggtttttgc atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg
      421 gttcgatgac cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc
      481 gattaatcac cttttttgac aaatcactac cgggaaaaaa caaacatttc caaaacaaat
      541 aactcacagt aattaacatc atcaggctta tttttatagt gaggataatc ctgatgatgc
      601 gcaccgtgct ttcatccatc gcgcgcaaaa atcattctct aagtaaatga atggattgca
      661 tgcgtttcac tcaatcgtac tttaattgac caatcccgct tattaacttt ctgtatcact
      721 ttttcttata aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg
      781 aactcgtttt gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca
      841 cagatttggc cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg
      901 cattttttta taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca
      961 aatctcaacc gattttgaga tatatcttgc gcgggatccc cgcgattgca gtagcggttc
     1021 tgctttctgc atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag
     1081 tgggtagtga aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta
     1141 atgtcgacgt aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc
     1201 gtctgggcgg cagcactaaa aaaggtatcg attgttctgg ttttgtacaa cgtacattcc
     1261 gtgagcaatt tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat
     1321 ctgtttcccg cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg
     1381 gacgccatgt cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg
     1441 gtgttattat ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc
     1501 gggttctcag ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt
     1561 taactgaaag cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt
     1621 ttccgcgtct tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat
     1681 ttcaggaaat taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc
     1741 ttacctgcat tgttgcaggc gtgatgattg cgatactggt gagttgcctt caatttttag
     1801 tggcctggca taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc
     1861 tcgataccta ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag
     1921 atacctgcca gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc
     1981 gtacgtttgt gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg
     2041 acattccact caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga
     2101 tcttacccgg cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc
     2161 ctttgctgaa aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct
     2221 tttatagttc acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc
     2281 tatctacctt ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg
     2341 aaactaaaat tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga
     2401 acgatgtgtg gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct
     2461 gctattacct gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc
     2521 gcgaacaatt ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgagtaacgg
     2581 gcctggaagt actgctacgc tggcggcatc ctgtcgcggg agaaattccc ccggatgcct
     2641 tcattaactt tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt
     2701 taattgcccg cgatgccgca gaattagaaa aagtgctgcc agtaggcgtc aaatttggta
     2761 ttaacattgc gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc
     2821 tcacttccct acccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc
     2881 tgaaagagca agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg
     2941 ctattgatga cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg
     3001 attatctgaa aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac
     3061 ccgtacttga cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttgctgaag
     3121 gggtcgaaac gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag
     3181 gctactggat tagtcgcccg ttaccgctgg acgattttgt ccgctggctg aagaaaccgt
     3241 atacgccgca gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg
     3301 attgcggcta atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc
     3361 tgtttatcgc tctgttcacc tttggtgtgc aggcgcaggc tatcaaggaa agctatgcct
     3421 ttgccgtgct gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc
     3481 ccgccgcgcc aaaaggtggg cagataacgt tgtcagccct cggcaccttc gatactttca
     3541 accgctatgc actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgctat
     3601 ttacgacttc cgatgacgaa ccaggcagtt attacccgct gattgctgaa agcgcacgct
     3661 atgctgacga ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg
     3721 gttcgcccat tactgcccgc gatgtagagt ttacttttca aaaatttatg accgaaggcg
     3781 tgccgcaatt tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg
     3841 tgcgcattga gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg
     3901 tatttccaga aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc
     3961 ttgccagtgg tccgtaccgc attacgtcct ggaaaatggg gcaaaatatt gtctattccc
     4021 gcgtgaaaga ttactgggca gcaaacttac cggtaaaccg cggacgctgg aatttcgaca
     4081 ccattcgcta cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg
     4141 cctttgattt gcgtatggaa aacgacgcca aaaactgggc cacgcgttat accggtaaaa
     4201 atttcgataa aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc
     4261 gctggctggc gtttaatatc caacgtccag tattcagcga tcgccgggtg cgggaagcaa
     4321 tcactctcgc ctttgacttt gaatggatga acaaggcgtt gttttacaat gcctggagtc
     4381 gcacgaacag ttattttcag aataccgaat acgcggccag aaattacccc gacgccgcgg
     4441 agctggtgct tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct
     4501 accagccgcc ggtatccaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca
     4561 aacttctcaa cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg
     4621 gtcagccact cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc
     4681 cgttccagca cagcctgcaa cggctgggta tcaacatgga cattcgcaag gtggataact
     4741 cgcaaatcac taaccgcatg cgcagtcgcg actatgacat gatgccgcgc gtatggcggg
     4801 cgatgccgtg gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca
     4861 cttataatgc ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg
     4921 ccgcgcaggg aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa
     4981 cgtggaatta ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg
     5041 ataaattctc ccagccggcc gtgcgcccca tctatagcct cggtatcgat acctggtggt
     5101 atgacgtcaa taaagcggcc aaactgccgt ccgccagcaa acagggagag tagatgggcg
     5161 cttacctgat tcgccgtctg ttgctggtga tcccaacatt atgggcgatt atcaccatca
     5221 actttttcat cgtgcaaatt gcgcctggcg gtccggtcga ccaggccatc gccgccattg
     5281 agtttggtaa tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg
     5341 cgcaaacggg tgtcggcaat atcagcgaca gtaagtaccg tggcggacgc ggattagatc
     5401 cagaagtgat cgctgagatc actcatcgct acggttttga taagccgatc cacgaacgtt
     5461 acttcaaaat gctctgggac tacatccgct ttgattttgg cgatagcctg tttcgcagcg
     5521 cctcggtgct gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga
     5581 gcacgctgat tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg
     5641 ggagccgctt tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct
     5701 ttttgtttgc catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc
     5761 ctctacgcgg cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg
     5821 attatctgtg gcatatcacg ctgccggtgc tggcgacagt gattggtggc tttgcggcgc
     5881 tgaccatgct gacaaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg
     5941 cgcgtgcgaa aggggtaagt gaaaaaaata ttctctggaa acatgtgttc cgcaacgcca
     6001 tgctgctggt gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc
     6061 tgctgattga ggtgatgttt tcactcaatg gtctgggctt actgggctac gaagcgaccg
     6121 tctcgcgcga ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc
     6181 tgctgaatat tgtcagtgat atcagctata cgctggtcga tccgcgtata gattttgagg
     6241 gacgttaatg tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgccataa
     6301 ccgtcgcggc tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc
     6361 tgaacttatc gccaacgata aaccgttgct ggtacgttat gacggcagtt ggtatttccc
     6421 gttgttgaaa aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta
     6481 tcaggacccg tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat
     6541 tcgctttggt gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc
     6601 ccggcaaaac tggctgggaa cggatgccaa cggcggcgat gtgctggcac gtattctcta
     6661 tggcacgcgg atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg
     6721 cgtgctggcg ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg
     6781 ctttattgaa gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt
     6841 cgtacagcct aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct
     6901 ggtcggcgtg gtgcgggcgg agtttttacg cacccgtaat ttcgactaca ttcgtgcggc
     6961 gcaggcgctt ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat
     7021 ggtcgcgacc ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc
     7081 actcgatttc ctcggcttcg gtctgccgct cggttcaccg tcactcggtg aactgctgtt
     7141 acaagggaaa aataaccttc aggctccgtg gcttgggatc accgccttct tgtcggtggc
     7201 gatattattg tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa
     7261 taaggcggtg tagcatgacg caaactctgt tagcgattga agatttgtcg gtgggttttc
     7321 gccatcagca aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg
     7381 aaacgctggc gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt
     7441 tacgcctgct cccttccccg ccggttgaat atctctccgg cgatattcgt tttcgtggcg
     7501 aatcgctgct tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca
     7561 tgatttttca agagccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt
     7621 atgaagtgct ttcactccac cgcgggatgc gtcgggaagc ggctcgaggc gaaattctta
     7681 actgccttga tcgcgttggt atccgccagg cggcaaaacg gctgacagat tatccgcatc
     7741 agctctccgg cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg
     7801 aattattaat tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt
     7861 tacagctgtt gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata
     7921 acctcagcat tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg
     7981 tcgagcaaaa taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc
     8041 tactcaacag tgaaccgtca ggcgacccgg tgccgttgcc agaacctgcc tcaacgttgc
     8101 tggatgttga acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg
     8161 tggatcataa tgtggtggtg aaaaacatca gttttacgct acgagcgggt gaaacactgg
     8221 gtttagtggg cgagtccggt tccgggaaaa gtacgacggg actggcgctg ctgcgactga
     8281 ttaattctca gggcagcatc atctttgacg gtcagccact gcaaaattta aatcgccgcc
     8341 agctgttacc tattcgtcat cgcattcagg tggtatttca ggatccaaac tcctcgctca
     8401 acccacgact caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc
     8461 tttctgccgc acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc
     8521 ctgaaacacg ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga
     8581 ttgccagggc attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac
     8641 tcgacaaaac ggtacaggcg caaatattga cgctattgaa atcattgcaa caaaagcatc
     8701 aactggccta tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg
     8761 ttatcgtact gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca
     8821 caccgcagca ggagtatacg cgtcagctac tggcgttgag ctgacgctta aaaaggattg
     8881 tagtttgaaa agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca
     8941 aactcgtcct ggcgatttac aaacaggcac ggctcacctt cacactccag caccgaacat
     9001 ggaacttcga tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca
     9061 ccatccgggc taagcaattt aagagccacc aggctgttgt caccgcacgg tccattttgc
     9121 ggaatcggtt caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt
     9181 cgatggacga ttgagagttg taatgaagtc acttattttc cccggaagca cattcacttc
     9241 acaatttgtt tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa
     9301 agattgatgc ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt
     9361 ggtcacgttt gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta
     9421 caatgatgtg actttttaca taaattgatt ttacataaaa taaacatata tcgggggaat
     9481 gatagatttg tggttgacat gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa
     9541 tagatcaccg ttttttcggt cgactggcgt acagacagaa gagaatggag ctgcttgcgc
     9601 agaatgcaat tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg
     9661 cgccaacaat tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg
     9721 ccatatgggg aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc
     9781 agcccacaaa cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac
     9841 tgatgaccat ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta
     9901 acgcgccaat gcggcgaacg aagcggctgt taaaaatggt catcacgaac aggaaaacaa
     9961 tgtttagtgc aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatgtaga
    10021 caaacggtcc ggcgcttaag aacgagaaca tcccggcaaa gctgaaacca ctggcaagca
    10081 tataactcag gacacgctta tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa
    10141 tgtgaaaagg ctggcgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg
    10201 ccgaagccag aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca
    10261 ccagcaccca accgccgaca atcggtgcca tcagcggtgc aatagtggtc accagcatga
    10321 caaacgacat catccgcgag aactcttctt tcgggtaaat atcgcgcatc agggcgttaa
    10381 tgaccacgct ggccgcagcc gccgccagcc cgtggaagaa acgcatcaca atcagctgat
    10441 cgatggtgtt tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtaccgccga
    10501 gcaccaccgg cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg
    10561 caaagcccag aatataagta ctgagggtca tctgcgtact gcccgcaggt acgccaaact
    10621 gcgctgaaat taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg
    10681 ccagcaggcc aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg
    10741 gctcctgaaa gtcatggaaa agttagacga cgctggcaat ttcttcttca gttaacggac
    10801 gatattcacc gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca
    10861 gctcaaccac gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt
    10921 cgctgatggt cagacgaacc tgcgttgggg taatcacttc cagcaccgca ggcttagtga
    10981 gatctttttc gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta
    11041 caggtgattc cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc
    11101 ggtgcgacca ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac
    11161 gtcctgcggc gtgcagtttc caggctaccg gttcatccag aaaatagagc accgttgggt
    11221 gatcagggtc gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg
    11281 gaccatgttg ctgcgccagc gggttgccat cgtaagcgac atcatgttca ggaagcagtt
    11341 tgaacgctgc attacgaaca atttcgccat cgacggtgac acgattgccg cggatttcac
    11401 gcccggcaat agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt
    11461 gtgtgatttt gcctgtaaaa acggaggtcg ggcattgacc cgaaaatctg aactgttgtc
    11521 tgcccagtat agcggtctaa taacatccct caagggaaaa agatccatgg catactatta
    11581 gcagaataat ctacctacgc gagaccatga tttttacact ccgcccatat cagcaagaag
    11641 ccgtggatgc cacgctcaac cattttcgtc gtcataaaac ccctgccgtt atcgtgctgc
    11701 ccaccggcgc aggtaaaagc ctggtgattg ccgaactggc acggctggcg cgtggtcgtg
    11761 ttctggtgct ggcacacgtc aaagaactgg tggcgcaaaa ccatgcaaag tatcaggcgc
    11821 tggggctgga agccgatatt tttgccgctg ggttaaagcg caaagagagc cacggtaaag
    11881 tggtatttgg cagcgtgcag tcggtcgctc gtaatcttga tgcctttcag ggtgaatttt
    11941 cgctgttgat tgtcgatgaa tgccaccgta ttggtgacga tgaagagagc cagtatcagc
    12001 aaatcctcac tcacctgact aaagtgaatc cccacttacg cctgctgggg ctgactgcca
    12061 cgccttttcg attgggcaaa ggctggatct accagttcca ttatcacggc atggtacgcg
    12121 gcgatgagaa agcccttttc cgtgactgca tttatgagct gccgctgcgt tatatgatta
    12181 aacacggcta tctgacgccg ccagaacgac tggatatgcc agtagtgcaa tacgatttca
    12241 gccgcttgca ggcacagagt aacgggctgt tcagcgaagc tgatctcaac cgtgagctga
    12301 aaaaacagca acgcattacg ccacatatca tcagccagat catggagttc gccgaaaagc
    12361 gcaaaggggt aatgattttc gccgccaccg ttgaacacgc aaaagagatt gtgggattac
    12421 tgcctgccga agatgctgca ctgattactg gcgacacccc cggcgctgag cgcgatgtgt
    12481 taattgaaga ttttaaagcc cagcgttttc gctatctggt caacgtcgca gtactgacca
    12541 ccggatttga cgccccgcac gtcgatctta tcgccattct gcgccctacc gaatcagtga
    12601 gtctttacca acaaattgtc gggcgcggtc tgcgtctcgc tccgggcaag actgattgtt
    12661 taattcttga ttatgcgggt aatcctcacg atctctacgc gccggaagtt ggtacaccga
    12721 aaggcaaaag tgacaacgtt ccggtacagg ttttctgccc tgcctgcggt tttgccaaca
    12781 ccttttgggg gaaaacgacc gccgacggga cattgattga acactttggt cgccgctgtc
    12841 agggatggtt tgaagatgac gacggtcatc gcgaacagtg tgacttccgt ttccgtttta
    12901 aaaattgccc gcaatgtaac gcagaaaatg atattgccgc tcgccgctgc cgcgaatgtg
    12961 acacggtact ggttgatccg gatgatatgt taaaagcggc gctacgactg aaagatgcgc
    13021 tggtattacg ctgtagcggc atgtctttgc agcatgggca cgacgagaaa ggcgaatggt
    13081 tgaaaatcac ctattacgat gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa
    13141 cgcccgccca gcgaactgcc ttcgagcagc tttttatccg cccgcatacg cgcacaccgg
    13201 gcatccctct gcgctggatc accgccgccg atatcctcgc ccagcaagcc ttactgcgac
    13261 atccggattt tgtcgtcgcc cgcatgaaag gtcagtactg gcaggtgcgt gaaaaagtgt
    13321 tcgattacga aggtcgtttt cgtcgggcgc acgaattacg cggttaatgg tagttttgat
    13381 tgatgtataa ggcgattgag tatagaatct cgcccgcttt tgcatacgca aagcagatca
    13441 cttacctgtt gctgggtcgc ctgtagcagg attaatttaa gagagaaaga aatgtttact
    13501 atcaacgcag aagtacgtaa agagcagggt aagggtgcga gccgccgcct gcgtgccgct
    13561 aacaagttcc cggcaatcat ctacggtggc aaagaagcac cgctggctat cgagctggat
    13621 cacgacaaag tcatgaacat gcaagctaaa gctgaattct acagcgaagt tctgaccatc
    13681 gttgttgacg gtaaagaaat caaagttaaa gctcaggacg tacagcgtca cccgtacaaa
    13741 ccgaagctgc agcacatcga cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc
    13801 ccgctccggc ggggtttttt gtatctgcag attatgcctg attacggtat tgctattttt
    13861 tgcaggccag ataaggcgtt cacgccgcat ctggcattca gaacacaaaa ccgattaatt
    13921 gccgccagac gtccggcgct gcaattggtc gcgcaaattc ggcggcgtgc ctttaatggt
    13981 caaggtatcg gttgctggat cccagaagat gcgttcacct aacagcatcg catcaaagtt
    14041 aatagtcaag ccaccgccgc taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc
    14101 tgccgggaag ctctcttcca gttcgtagcc tttttcagcc gcaaactccg tgaagctcac
    14161 ttcgcttacg cctgccagct ctttcgacag cgattccagt tcgatctctt cgcctgcctg
    14221 cagctgctca ttacagtagc tataaacctg ctggcgcacg ttctgtcgct ctgctttatc
    14281 cagttgtgct tcggcagtga aatcatcaac cgcttgcaac agcccacggt tttgcgcttt
    14341 cgcgttcaat ccttcgctgg ccccgaggaa atccataaag aaatccgcca ctttgcgccc
    14401 tactcgccct ttcaggaaag tgagatagcg ggtggactct ggattggttt cccattcggt
    14461 taaatcgata cgcgcaacaa tatccgcatg attgatatca agataatggg ttgggttgat
    14521 gtcgagattt tcgttaacgc gcatactgct caggttgctc agaactgcca ccagcagata
    14581 ctccaccgcc agataacggt agtggcaaaa cagcacgaat ccaccgtcag caaaaggata
    14641 tttcgctaat tcgtcacgca ggcgcccggt tgccgcgcgg ctaaatgcca gaaagtcctc
    14701 ttcgccctga cgctgtaagc gcagcgtctg cgccagttca ctctcttcgc taaacaagcc
    14761 gtaagcttta tttttggcgc tatagacccg atgcagttct gccaccatct caacgacggt
    14821 ttctgtcggt tccagcaatg aatcgcgcaa caccagctca aggttctgct catcacgctt
    14881 gataagctgg tgcagggcaa tctggttgat atccagactc atgataaact ctcctttaag
    14941 accgggcggt attcaaccac cgccggttaa gcgacgcaat aaaagatggc ggtcatttta
    15001 cagaaggcag cgtaacgaga atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct
    15061 acggtaatat gttgcccttt catcaactaa ctgatttcga tttatgccac aaatttcccg
    15121 ctacagtgat gaacaggttg aacaattgct cgcagagctg ctcaacgtac tggaaaaaca
    15181 taaggctccg accgaccttt ccctgatggt gttaggcaac atggtcacta accttatcaa
    15241 caccagcatt gccccggccc aacgccaggc aattgccaac tcttttgccc gcgccttaca
    15301 gtcctctatc aacgaagaca aagcgcacta agggaaacag ataactggtt atggtaactc
    15361 atcgtcagcg ctaccgtgaa aaagtctccc agatggtcag ttgggggcac tggtttgcac
    15421 tgttcaatat tctgctttcg ctcgtcattg gcagccgtta cctgtttatc gccgactggc
    15481 cgacaacgct tgctggtcgc atttattcct acgtaagcat tatcggccat ttcagcttcc
    15541 tggtgttcgc cacctacttg ctgatcctct tcccgctgac ctttatcgtc ggctcccaga
    15601 ggctgatgag gtttttgtcc gtcattctgg caacggcggg aatgacgcta ttactgatcg
    15661 atagcgaagt ctttactcgt ttccatctcc atcttaatcc catcgtctgg caactggtta
    15721 tcaacccaga cgaaaatgag atggcgcgcg actggcagct gatgttcatc agcgtgccgg
    15781 ttattttatt gcttgaactg gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga
    15841 cgcgtcgtcg acgcttcgcg cgcccgctgg ccgcattctt atttatcgcc tttatcgcct
    15901 cgcatgtggt gtatatctgg gccgatgcca acttctatcg cccgatcacc atgcagcgcg
    15961 ctaacctgcc gctttcgtac ccgatgacgg cgcgacgttt tcttgagaag catggtctgc
    16021 ttgatgcgca ggagtatcaa cgccgtctta ttgagcaagg taatccagac gccgtttccg
    16081 tacagtatcc gttaagcgaa ctgcgctatc gcgatatggg caccgggcag aatgtgctgt
    16141 tgattactgt cgatggcctg aactactcac gcttcgagaa gcagatgcct gcgctggcag
    16201 gttttgctga gcaaaatatt tcgttcacgc gccatatgag ctccggcaac actacagaca
    16261 acggcatctt tggcctgttc tatggcatct cgccgagcta tatggacggc attctgtcga
    16321 cccgtacgcc tgcggcatta attactgcgc ttaatcagca aggctatcag ctggggttat
    16381 tctcgtcaga tggctttacc agcccgctgt atcgccaggc attgttgtca gatttctcga
    16441 tgccgagcgt acgcacccaa tccgacgagc agaccgccac gcagtggatc aactggctgg
    16501 gccgctacgc acaagaagat aaccgctggt tctcgtgggt ctctttcaat ggcactaaca
    16561 ttgacgacag caatcagcag gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg
    16621 atgaccagat caaccgcgtg ctcaatgcac tgcgtgattc tggcaaacag gacaatacgg
    16681 ttgtgattat cactgccggt cggggtattc cgctgagcga agacgaagaa acctttgact
    16741 ggtcccacgg tcatctgcag gtgccattag tgattcactg gccaggcacg ccggcgcagc
    16801 gtattaatgc gctgactgat cataccgatc tgatgacgac gctgatgcaa cgcctgctac
    16861 atgtcagcac acctgccagc gaatattcgc aaggtcagga tttgttcaac cctcaacgcc
    16921 gtcattactg ggttactgca gcggataacg atacgctggc aattaccacc ccgaaaaaga
    16981 cgctggtgct gaacaataac ggtaaatacc gcacttacaa cttacgtggt gaaagagtga
    17041 aagatgaaaa accacagtta agtttgttat tgcaagtgct gacagacgag aagcgtttta
    17101 tcgctaactg attaattata aatcagttag cgaaatatct tacttgcaat cggtgtggaa
    17161 aacggtagta ttagcagcca cgagtcggca cgtagcgcag cctggtagcg caccgtcatg
    17221 gggtgtcggg ggtcggaggt tcaaatcctc tcgtgccgac caaatcgccc accggatatc
    17281 accggaaagc accggataag acagggataa cccgctaatc acttgattgg cgggtttttt
    17341 tatgtttttt agtcaccgaa catcaccgga tttataagct atcagccgga ttttttagtt
    17401 atacgtttag ttatacgggc tggcgtataa ctaaagccgt ataactaaaa ccctgttttt
    17461 tcgtgtggaa tcatagacat atggcgagaa aaacacaccc attaaccaca gtgcagatca
    17521 aagcagccag accagcgcaa aaggagtaca ccctgcagga cggcggaggg ctttttctcc
    17581 tggtcaaacc gtctggatca aaactctgga gattttccta ctaccgacca tcggacaaaa
    17641 aaagaatatt gctgagtttt ggatcgcttg aagatgtttc cctggctgat gccagaaaac
    17701 gccgtagcga gtacaggacg ttaatcagtg ccggaactga cccgcaggac cacgagaggc
    17761 aaaaaagaga gacagaggcc cgaagacaag ggaacacgtt cgaaaatgtg gcggcggcat
    17821 ggtaccaggt gaaaatcagc cagaatctgg cccccaacac gattaaagac atctggcgtt
    17881 cgctggataa atatgtattc ccgttcatcg gcaacacgcc aatagatacc ctcaccgccc
    17941 gaaggttcgt tgaagtgctt acccccatca aggagcgcgg caacctggaa acactcaagc
    18001 gggttttaca gcgcgttaat gaggtaatgg attacgccgc caacagtggg ctgattgatg
    18061 ccaatccggc tatgaatgtg cgtaaggcgt ttccctcacc tgtaaaaaaa catatgccaa
    18121 caatccgccc cgaacagctg cccgagctta tgcaggcttt atcagtatcg gcaacagaac
    18181 ggcagaccag attactgatt gaatggcagt tactgaccgt aacccgtccc gccgaagcgt
    18241 catcaacgcg gtgggatgaa atcaacctgg acgcgaagca atggacgata cctgccggac
    18301 gcatgaagat gcgcagggat cacgttatcc cgctttccgg tcaggctatg gcggtgctgg
    18361 aggccatgaa accaatcagc caccaccgca attacgtttt cccaagtctg aaagacccac
    18421 agcagccgat gaacagccag acagctaacg cagcattgcg gcgtatggga ttcgctggcg
    18481 tgctggtgtc tcatggatta cgcgccatat tcagcacagc agcgaacgag gaaggattcg
    18541 agccggacgt aatagaggcg gcacttgccc acgtcgacac caacgaagtt agacgggcat
    18601 acaaccggag caactacata gaaaaacgca tcgtgctgat gcgctggtgg ggcgaatttg
    18661 tcgaggctgc ggcgacgggc gtaaccctcg ccagtggtaa aaggggtatc cgagccgtgt
    18721 agctgtacag aaaaccagta aaaactacga aaaccatgta aaaccgtcgt ataattgcat
    18781 caaatttaac gacaaggccg tgaaacatga aaccgttaag atgcaaaaaa atatcagatg
    18841 caattgcgac gggctgcaac tggccctgat ggttcagcat gaattttggt caacctacga
    18901 tccggaggac agaacgacgg ccccatcaaa aaaacaggta gtagattttc tggtatcccg
    18961 tggcgcttcc agaaatctgg cggtaagtat tgataaggtc gtacgtccgg catctatgaa
    19021 gatcggaggc aggcccaaaa aatggcggta acaatcctgg aagcggcaga aatgccgctt
    19081 tttttataat tccatttcaa atcatcaata taaaaaacgg tgtataccgt ttaaaaacgg
    19141 tgggaactgt ttttacccat atccgatgat ttaccgtatt tgtcaccgga atacaccgga
    19201 ttcacaaggt aaatcacgat ggaagcaatc agaaaaatta tctttcgcca ggaagtaaaa
    19261 aaaattatcc acatcaaggc agacagcacg ctgcaaagca tgatcaacgc cggagaattt
    19321 ccgcagggtt ttcgcgttgg tttacgccgt cgcggatggt atgaggatga tgtgttgtcc
    19381 tggcagaaag aacgcgaaga ggaagcacgc ggaacggctg cttaacgggt atcacagata
    19441 tgacaaacac gaaaaaagcg gccccgatat ggagccgcct ttctgaacaa ttaacccgct
    19501 gcgccttatg tgtatgtgat cccaaacata agcacgggga tgatagccgc tatcaggctg
    19561 gtgggcaatg caatcagtct ggttcagttc gttgccatac ctgcaatgag cgcttttccc
    19621 tgtactcttt aaggaattgc tcaagggcaa aagcacatgg cgcgaatctt tctgattcat
    19681 gctctatctt tctgcgccgt cttttccgtg ccggtgataa tgttttggtc aattctttat
    19741 cggtcattgt gttgtcctgc atagcaatgc gccgtaatac ctcacaccac ggcgctgata
    19801 gtttttatcc tttgggttct atgccgcgtt gctgtaattc tttgcggaga acgcgtttaa
    19861 tccaagtggc taaagatgtg tcgccatcgg ccttagctgc ttcttctagc tgcgctctga
    19921 agtcttccgg taatcgcatt tggtatggag gcgaacgttt ttctaatggt gttgacatgg
    19981 taattaccct tcattactat acctacatgg taattaccat tgtacttgcc attacacaaa
    20041 aatcaacgcc ccgcagtgct gggaacacat acggggcgtc taaccaacaa cgtaaactag
    20101 gagccgttat ggttgccgta aatcatatac cacaccttgt acacacacaa acggcctttg
    20161 tgtggcgttt tctggccctg aatatcggag aaaaaaacca gttgatcgcc agtgtcgaaa
    20221 atagctatga ttcccgcgca cctcataaaa cgggtgccgg gattggcgtc ctgggcaaaa
    20281 ctgaagcgca taacacgcgc cccgcgtgtt tttttgtgcc gcatagtcac accttatcaa
    20341 tggtgggctg tacgggggcg gagcaatccg cgccggtttc ttcagtgtcc ggttacgcca
    20401 accctgtaca gtccgccacc agcgaaattg gcgtttccgg tggcggttat cttaaccaca
    20461 ctgaggaggc tgccaacatg gctactatcc ctacccttgt acattctcaa accgcctttc
    20521 tctggcgctt tatcatcttt ggcgcgtcag aacatcaaat catccacgta accgcctgga
    20581 cggaacgcga agcgcgtagc cgttgcccgt ccggttgtgt tgctgtattc gccgcccgta
    20641 ttcgtcagga ggtgtgccat gtgcaataaa accacaccgg acgcagcagc cgccgcgctc
    20701 actacgctga tgcacgcgct tatcgatatt gaatgcactg cagagcttgc gcagggagaa
    20761 gaacagaaag accggacaca gttcgccctg gaatgtatcc gatacatcgc aacgcggtcg
    20821 ctgaatgacg ctaaaaatat tcttgttgct gattgtgaaa atgggggggg ttatgcgtga
    20881 tgatcgtttt aattccctga aacaagaatt ttccggcgtt cctgatgatg cggctgatgc
    20941 gctttcgtca atatctgaaa ttatgcgggt ggcttttttc tttctttgca ctgatgagca
    21001 cagagataca gggctaaata ttcttgatat tgccgctaac tatgctgatt tcgtgacaga
    21061 agctgtttta agaaaaacaa cggacgggga ttaatatgcg tgatatttac cacgaaacaa
    21121 tagaccgcgc atttagtgcc cttgcttacg ctgaaggtat gtacgaaata ttgcgcatat
    21181 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gtcacggcat
    21241 taataacgct tcttgagcct gtaataaatg agttgcagga aatagagaca ttgcacgaca
    21301 gatataa
//
LOCUS       DAZKGS010000071        20770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKGS010000071 DAZKGS010000000
VERSION     DAZKGS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..873
                     /gene="exuR"
                     /locus_tag="ACHOQY_002649"
     CDS             97..873
                     /gene="exuR"
                     /locus_tag="ACHOQY_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9678724.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1113..1196
                     /gene="yqiM"
                     /locus_tag="ACHOQY_002650"
     CDS             1113..1196
                     /gene="yqiM"
                     /locus_tag="ACHOQY_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP9678725.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1218..1880
                     /gene="yqjA"
                     /locus_tag="ACHOQY_002651"
     CDS             1218..1880
                     /gene="yqjA"
                     /locus_tag="ACHOQY_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9678726.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1884..2267
                     /gene="mzrA"
                     /locus_tag="ACHOQY_002652"
     CDS             1884..2267
                     /gene="mzrA"
                     /locus_tag="ACHOQY_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9678727.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2414..2782
                     /gene="yqjC"
                     /locus_tag="ACHOQY_002653"
     CDS             2414..2782
                     /gene="yqjC"
                     /locus_tag="ACHOQY_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9678728.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2820..3125
                     /gene="yqjD"
                     /locus_tag="ACHOQY_002654"
     CDS             2820..3125
                     /gene="yqjD"
                     /locus_tag="ACHOQY_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9678729.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3128..3532
                     /gene="yqjE"
                     /locus_tag="ACHOQY_002655"
     CDS             3128..3532
                     /gene="yqjE"
                     /locus_tag="ACHOQY_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9678730.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3522..3821
                     /gene="yqjK"
                     /locus_tag="ACHOQY_002656"
     CDS             3522..3821
                     /gene="yqjK"
                     /locus_tag="ACHOQY_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9678731.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4007..4399
                     /gene="yqjF"
                     /locus_tag="ACHOQY_002657"
     CDS             4007..4399
                     /gene="yqjF"
                     /locus_tag="ACHOQY_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9678732.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4469..5455
                     /gene="yqjG"
                     /locus_tag="ACHOQY_002658"
     CDS             4469..5455
                     /gene="yqjG"
                     /locus_tag="ACHOQY_002658"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9678733.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5748..6113
                     /gene="yhaH"
                     /locus_tag="ACHOQY_002659"
     CDS             5748..6113
                     /gene="yhaH"
                     /locus_tag="ACHOQY_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9678734.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6355..6711
                     /locus_tag="ACHOQY_002660"
     CDS             6355..6711
                     /locus_tag="ACHOQY_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9678735.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6762..7658)
                     /gene="yhaJ"
                     /locus_tag="ACHOQY_002661"
     CDS             complement(6762..7658)
                     /gene="yhaJ"
                     /locus_tag="ACHOQY_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9678736.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            7763..8464
                     /gene="yhaK"
                     /locus_tag="ACHOQY_002662"
     CDS             7763..8464
                     /gene="yhaK"
                     /locus_tag="ACHOQY_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9678737.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8487..8651
                     /gene="yhaL"
                     /locus_tag="ACHOQY_002663"
     CDS             8487..8651
                     /gene="yhaL"
                     /locus_tag="ACHOQY_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9678738.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8863..10173)
                     /gene="cyuA"
                     /locus_tag="ACHOQY_002664"
     CDS             complement(8863..10173)
                     /gene="cyuA"
                     /locus_tag="ACHOQY_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9678739.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10201..11532)
                     /gene="cyuP"
                     /locus_tag="ACHOQY_002665"
     CDS             complement(10201..11532)
                     /gene="cyuP"
                     /locus_tag="ACHOQY_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9678740.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11807..13171)
                     /gene="tdcG"
                     /locus_tag="ACHOQY_002666"
     CDS             complement(11807..13171)
                     /gene="tdcG"
                     /locus_tag="ACHOQY_002666"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9678741.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13243..13632)
                     /gene="tdcF"
                     /locus_tag="ACHOQY_002667"
     CDS             complement(13243..13632)
                     /gene="tdcF"
                     /locus_tag="ACHOQY_002667"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9678742.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13646..15940)
                     /gene="tdcE"
                     /locus_tag="ACHOQY_002668"
     CDS             complement(13646..15940)
                     /gene="tdcE"
                     /locus_tag="ACHOQY_002668"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9678743.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15974..17182)
                     /gene="tdcD"
                     /locus_tag="ACHOQY_002669"
     CDS             complement(15974..17182)
                     /gene="tdcD"
                     /locus_tag="ACHOQY_002669"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9678744.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17208..18539)
                     /gene="tdcC"
                     /locus_tag="ACHOQY_002670"
     CDS             complement(17208..18539)
                     /gene="tdcC"
                     /locus_tag="ACHOQY_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9678745.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18561..19550)
                     /gene="tdcB"
                     /locus_tag="ACHOQY_002671"
     CDS             complement(18561..19550)
                     /gene="tdcB"
                     /locus_tag="ACHOQY_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9678746.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19649..20587)
                     /gene="tdcA"
                     /locus_tag="ACHOQY_002672"
     CDS             complement(19649..20587)
                     /gene="tdcA"
                     /locus_tag="ACHOQY_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9678747.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         5360 a         5422 c         4932 g         5056 t
ORIGIN      
        1 cattaattca attaccaaat ttatatatga ccaatggcat acccatgaat aaagtggtat
       61 gataacttcg tccgcattcc actccgggag acgcatatgg aaatcactga accacgccgt
      121 ttgtatcaac aacttgccgc tgacctgaaa gagcgcatcg aacagggcgt ctatctggta
      181 ggtgataaac tgcctgcaga acgctttatt gccgatgaaa agaacgtcag ccgtacggta
      241 gttcgtgaag ccatcatcat gctggaagtt gaaggctatg tcgaagtgcg taaaggttca
      301 ggtattcatg tggtttccaa ccagccgcgc catcagcagg cggctgacaa taatatggaa
      361 ttcgccaatt acggtccgtt tgagttgctt caggctcgcc agctcatcga aagtaatatt
      421 gccgagttcg cggcaactca ggtaacgaaa caagacatca tgaaactgat ggccattcag
      481 gaacaggcgc gcggcgaaca atgcttccgt gattccgagt gggatttaca gttccacatt
      541 caggtagccc tggcgacgca gaactccgcc ctggcggcca tcgttgaaaa aatgtggacc
      601 caacgtagcc ataacccgta ctggaaaaaa ctgcacgaac acattgattc ccgtaccgtc
      661 gataactggt gtgatgacca cgatcaaatc ctcaaggcgc tgattcgcaa agatcctcat
      721 gctgctaagc tggcaatgtg gcagcacctg gaaaacacca agatcatgtt attcaacgaa
      781 accagcgacg acttcgagtt caatgccgac cgctatctgt tcgccgaaaa cccggtagtg
      841 catctcgata cggccaccag cggcagtaaa tgaaatacct gtccgacagg cgctgcgacg
      901 cgcctgtttg tctcgtcggt aagcgaaaag tataaagtgt cagcctgtgt aaatcctctc
      961 gccaccctcc cctgcattca gcaaaatcag actccacgga catgtaattt tgataacgaa
     1021 caacgttgac ctttgttaca attagattca atttgaattt atgtttttga atgctttctt
     1081 atctcacgat ttaacaggga atagttcagg ctgtgttgat gtatcaaacc cgcagaacat
     1141 accaaaacag caataacatt gcggtagtgc atcttttaaa accagcgtgg cgttaaccga
     1201 ttcaccagga ataatgaatg gaacttttga cccaattgct gcaagccctg tgggcgcagg
     1261 attttgaaac cctggccaat ccatcgatga ttggcatgtt gtattttgtc ttgtttgtaa
     1321 ttttgttcct tgaaaacggc ttgcttccag cggccttttt accgggcgac agtttactgg
     1381 tattggtcgg cgtgttgatt gcgaaaggcg cgatgggtta cccgcaaacg attctgctgc
     1441 tgaccgttgc cgccagcctc ggctgctggg tcagctatat tcaggggcga tggctgggca
     1501 atacccgcac cgtacaaaac tggctatctc atttacccgc gcattatcat caacgcgcac
     1561 accatctttt tcataaacac ggtttatcgg cgctgttaat tggtcgcttt attgcgtttg
     1621 tcagaacact gctgccgacg attgccgggt tatcagggct gaataacgcg cgctttcagt
     1681 ttttcaactg gatgagcggt ctgctgtggg tattgatcct gacaactctg ggttacatgc
     1741 tcggcaaaac gccggtattt ttaaagtacg aggaccagct gatgtcatgc ctgatgctgc
     1801 tcccggtggt gctgctggtg tttggcctgg caggttctct ggtcgtgtta tggaaaaaga
     1861 aatatggaaa tcgggggtaa gggatgcaaa tacctcgcat gtcgcttcgc cagctagcct
     1921 ggtccggcac tgttttactt ctggtcggca cgctgttgct ggcctggtcc gcggttcgcc
     1981 agcaagagtc tacgctggcg attcgtgccg ttcatcaagg cacaacgatg ccagacggtt
     2041 tttcgatctg gcatcacctt gacgctcatg gcattccttt caaaagtatc acccccaaaa
     2101 acgacactct gttaattaca tttgactcca gcgaccagag cgccgccgca aaagcggtcc
     2161 tcgacagaac attgccccat ggctacatca ttgcgcagca ggacaataac agtcaggcta
     2221 tgcagtggct gacccggtta cgggataact ctcatcgctt cggataactt cctgttatcc
     2281 gaaacatatc actcactttg gtgatttcac cgtaactgtc tatgattaat gaggcggtgg
     2341 ttaacactac cgcttcgttg tctggacaat cgttcctttg taataggtcc gaatcacaat
     2401 ggaaggttca agaatgaaat accgcatcgc tttagctgtt tctctctttg ctcttagtgc
     2461 cggtagttat gccactaccc tgtgtcagga aaaggagcaa aatatcctta aggagatcag
     2521 ctatgccgaa aaacaccaaa accagaatcg tattgacggt ctgaataaag ccctgagtga
     2581 agtccgggcc aactgttcag atagccagct gcgtgccgat catcacaaga aaatcgcaaa
     2641 gcagaaagat gaggtggcgg aacgccagca agatttagcc gaggcgaagc aaaaaggcga
     2701 tgccgataag attgccaaac gcgaacggaa actggcagaa gcgcaggaag agctgaaaaa
     2761 gctggaagcg cgcgactact aactcacaat agtcactact tactcacctg gagaaaacta
     2821 tgtcgaaaga acacactacg gaacatctgc gtgctgagtt gaaatccctt tccgatacgc
     2881 tggaagaggt gcttagctca tctggcgaga agtcgaaaga agagttgagt aagattcgta
     2941 gcaaagcgga gcaggcactg aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca
     3001 tcgccaaaca aacccgtgtc gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt
     3061 ggaccggcgt gggcattggc gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc
     3121 gttaattatg gcggacactc atcacgcgca agggcccggt aaaagcgttc tgggcatcgg
     3181 gcagcgaatt gtttccatca tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt
     3241 ggagctggaa gaggaaaaag ctaatctctt tcaactttta ctgatgctgg gcctgacgat
     3301 gcttttcgct gcatttggtc ttatgagcct gatggtgcta attatttggg cggttgatcc
     3361 gcaatatcgc ctgaatgcga tgattgccac caccgtggtg ttgctgctac tggcactaat
     3421 tggcggtatc tggacgttac gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca
     3481 tgagctggca aacgatcggc aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac
     3541 gtgaacgacg taaggcgcaa ctgcttagcc agatccagca acaacggctg gatctttccg
     3601 ccagtcgtcg tgaatggctg gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc
     3661 taagtctgcg ctcctgggcg ctggttggca gtagcgtgat ggcgatctgg acgattcgcc
     3721 atcctaatat gctggtccgc tgggccagac gcggttttgg cgtatggagc gcctggcgtc
     3781 tggttaaaac gaccctcaag cagcaacagc ttcgcggtta acttccctct ggccggagca
     3841 attccggcct tatccctcaa atttcttgaa gatttttgac agttttcctt gctaacaatc
     3901 atcattcacc acgtttatga ttctctccat cgacagcaac gacgctaata ccgcgccctt
     3961 gcacaaaaaa acaatcagca gcctgagtgg cgcagtggag agtatgatga aaaaattaga
     4021 agatgttggt gtactggtag cgcgcatttt aatgccgatt ctgtttatta ccgcaggctg
     4081 gggaaaaatc accgcttact ctggtaccca acaatatatg gaagcaatgg gtgtcccggg
     4141 ttttatgctg ccgctggtga ttctgcttga gttcggtggt ggtctggcaa tcctgttcgg
     4201 tttcctgact cgcaccacag ccctgtttac tgcgggcttt acgctgctga cggcattttt
     4261 atttcacagc aactttgctg aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat
     4321 ttctggcgga ttcctgctgc tggcaattac cggtccgggc gcgtatagca tcgaccgcct
     4381 gctgaataaa aagtggtaag cacgcgctat acttaacgat aaaaaacgag gaggaagctc
     4441 ctcgtttttg ctattggagg agagaaaaat ggggcaactg attgacggcg tctggcatga
     4501 cacctggtac gataccaaat ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg
     4561 taactggctc actgccgatg gcgcacctgg ccccactggc acaggcggtt ttatcgcaga
     4621 gaaagatcgt tatcatctct atgtttcact cgcctgcccg tgggcgcacc gcacgctgat
     4681 catgcgcaaa ctcaaaggac tggaaccgtt tatttccgtt tccgtagtga acccgctgat
     4741 gctggaaaac ggctggacct ttgatgacag ttttccggga gcaaccggag atacgctcta
     4801 tcaacatgaa tttctgtatc agctttatct ccacgccgat ccacactaca gcggacgagt
     4861 tactgttccc gtgctgtggg acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga
     4921 aatcatccgc atgttcaata ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta
     4981 cccaccagcc ctgcaaacga aaattgacga acttaacggc tggatttatg acaccgttaa
     5041 caacggcgtg tataaagctg gttttgccac cagccagcaa gcctacgacg aggcggtggc
     5101 gaaagtgttt gaatcgctgg cgcgactgga gcagatttta ggccagcatc gttacctgac
     5161 cggcaaccag ctaaccgaag ccgatattcg cctgtggacc acgctggtcc gttttgatcc
     5221 agtgtatgtg acccacttca agtgtgataa gcaccgcatc agcgattacc ttaatctgta
     5281 tggcttcctg cgtgatatct accagatgcc gggaattgcc gaaacagtca atttcgatca
     5341 catccgtaat cattacttcc gcagccataa gaccatcaac cctacgggga ttatttcaat
     5401 tggtccatgg caggatctcg atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa
     5461 ggcaccttac gaggtgcctc tgataattag ctaagtgcat gttgttcatg tcggatgcgg
     5521 cgtgaacgcc ttatcaaacc tacaaaacat ttatattcaa tatgttgcaa tgaccgcgca
     5581 ggccagataa gcgtagcgca tctggcgatt ttgcgcttgt catcagtctt agcgcgcctt
     5641 cacagcactt tattaattcc ttgaacgaat atttactgcc atttcattca cgtctattct
     5701 taatttgctg ctttaaaaac aagtgattga gcaaaattga ggcaaaaatg gactggtatc
     5761 tgaaagtact aaaaaattat gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga
     5821 tgtttattct ggtcaacatc atctttacgt tcgtgctggg gctgctggat aaaatgttag
     5881 gctggcaacg cgctggtggc gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt
     5941 taccgtggtg ggcagttcag ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg
     6001 cactgttgtt cttaatcccg tttatcggct ggcttattat catcgtcttt aactgccagg
     6061 cgggtacgcc aggcgaaaac cgctttggac cagatccgaa actggaacct taaaacaaat
     6121 ctgctcgccg gatgtctgac tcatccggct tttatcatca tgttgatgaa tgaataattt
     6181 actaatgttt atttaaaata tttcaatgtc gttatataaa ttacctataa aaaacaacca
     6241 tgtatttatc ttaaatatca aaacctttat aacatccgaa aaagaaagat acgaatagca
     6301 tttacacaat acccggaata gactttcgtc acttttaatt aaagggatgt ttttatgcag
     6361 tggtatttag ccgtattaaa aaattatgtt ggtttctctg gtcgcgcacg tcgtaaagag
     6421 tactggatgt ttactctgat taacgccatc gtcggcgcta ttatcaatgt cattcaattg
     6481 attttaggtt tagagtttcc attcctttct ctcatttacc tggcagcaac aatcattccg
     6541 gtcattgctc tgtgtgttcg tcgtctgcac gatactgacc gttcaggtgc gtgggcgctt
     6601 ctctatctgg taccgatcat tggctggtta gttctgtttg tatttgcctg tttagaaggc
     6661 aattctggca gcaatcgtta cggaaacgat cctaagtttg gttcaaatta atcttagaat
     6721 tggggcgaca tttcgcccct ttttattaat aataacaaat attattttcc gctaaaaagt
     6781 ttgggaattt cccgcagaca ccaggatttc gcttccccca tactgtcacg acgccaggcc
     6841 ataataatat cgatctcgct ggtcgattcc gggctgacga cacgcaaccg cccttccgca
     6901 atatcttttt cgaccatcgg atacggcatc gtcgccacgc caagccccgc cagtaatgcc
     6961 tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct gtttgtccag cagctgtacg
     7021 gtcaacaccg ggcgctcacg agcggtatcc gctaccgcaa ttccacgata tttcacgcgc
     7081 gtcacttcag ataacggttc cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag
     7141 acgttcatta acgtatagag cttgcgcgag ttgatctccg acgaggaacg aaaatgcata
     7201 tccggcgcga taacaatatc cgcccgcccc tgctccagtc gttcccacgc ccccgccagc
     7261 acttcggtga tgattgccag ttgggtattg gcttttgccg ccagtttgtc gattaacggg
     7321 aaaaaggcag gtgtcggtac cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg
     7381 cgcgcgaggg cttccgcatc ggtagtcagt ttatctgcgg cttccagcaa aacgcgccct
     7441 cgctccagca acatcctccc gacattggtg aatttggtgc gatggcccga gcggtcaaac
     7501 agcaccacat ccagctcttc ttccagtttt tgcatggtgt agctaagtgc agaaggcacg
     7561 cgtcccagct catccgccgc cgccgcaaaa ctgccccggc gatcgatcgc atccataacc
     7621 cgtagtgctt ccagcgttaa tgccctttct ttggccattt cgttctcatt caggaaattt
     7681 gaacataccg ggcagaatat ctggctaaca atgtagcgtc cagcccctta ccataaaagg
     7741 aagtaaagag aggtcaagaa ttatgattac tacccgaact gccaggcagt gtggacaagc
     7801 agactacgga tggttgcagg cccggtatac tttttccttt ggacactact tcgacccgaa
     7861 attgttaggc tatgcctccc tgcgtgtgct taaccaggaa gtgctggccc cagatgccgc
     7921 ctttcagccg cgaacctatc ccaaagtcga tattttaaat gtgattctgg atggagaagc
     7981 agagtatcgc gacagcgaag gcaatcatgt tcaggccagc gccggtgagg cgttgctgct
     8041 ctctacccag ccgggtgtca gctacagcga acacaatctc agcaaagaca aaccgttaac
     8101 acgaatgcag ctttggctgg acgcctgccc gcagcgagag aatccgctga ttcaaaaact
     8161 ggcgcttaat atgggcaagc agcaattaat cgcctcgcca gagggtacga tgggaagcct
     8221 gcaattacgc cagcaagtgt ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa
     8281 tttccagttg catgggccac gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct
     8341 tacgcatcat gaagagaaag cggcgctgac ctgcggtgat ggggcgttta ttcgtgacga
     8401 ggctaacatt acgctggttg ccgattcccc actgcgcgct ttgctgatag atttgcctgt
     8461 ctagttgttt ttacagggag atgatgatga gtaaaaaatc ggccaaaaag cgccagccag
     8521 tgaagcccgt ggtggcgaaa gaacctgctc gcaccgccaa aaattttggc tatgaagaga
     8581 tgttgagcga gctggaagcc atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag
     8641 ctaccgcgta aagacaggtt gccggatgcg gcgaaaacgc cttatccggc ctacaaaatc
     8701 caacaacatt aacgaattaa acaacccgta ggccggataa gatgcgccag catcgcatcc
     8761 ggcatcaggt acaaaacaca acaacatcaa caagttgagc accccgtagg ccggataagg
     8821 tgcgccagca tcgcatccgg cacgataccc aaaacctggc gtttatctgg ccttgctcgc
     8881 cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta acgcacacag
     8941 gttggcaatc gactgctcaa catcatgcgc cacgatccct tcattgccgg tcacggcggt
     9001 atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg aaaccttcat
     9061 cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga tcatactgct
     9121 gatcgccatc gagatggttt cataacgccc atccaccagc catgccatcc cggcggcggc
     9181 ccccattgct gcggtcgttg cggcacacag cgcagacaaa cgcggtaact ggttatggat
     9241 gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agccgttcat catccgctcc
     9301 gaagtgttct gctaccacca ccacaggcat tgttgcggtg atcccctggt tacccgagcc
     9361 ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt
     9421 acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgctcgc actgtttttc
     9481 cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg ataacgcaca
     9541 atttagcttc gcggaatcga gaataaagcg gatcgccgca aacgggactt cattgacgaa
     9601 cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggagact cttgctcgcc
     9661 ctctgccaca cacgcctgct gggtaaacac cacaccattg tgcgtctcaa tatgcacaat
     9721 gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc agactttggc
     9781 gcgtgagaag aggatttcat tgcaaggttc ctggatctta acggagactt tccccgccgc
     9841 cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca cttccagccc
     9901 ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc ccaccattcc
     9961 cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc
    10021 tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca gcgccagtga
    10081 aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga ggatgtaacg
    10141 ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa
    10201 tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa ccagtgacga taatcaggta
    10261 cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc acgccgggat
    10321 caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca acaccggtgc
    10381 gttcagtacg atggcgctcc aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac
    10441 aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca ttacgatccc
    10501 ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga cagcaaagat
    10561 attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc
    10621 ggcaatcgcc agcgcggaaa tattctgctc ataggctttt accgcttcgt catgtcccat
    10681 cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat
    10741 attcattgcc cgcaatgctt tatgccgcgc cacttcaatt gatttttccc gcgagcgata
    10801 agagatcacc atcgggctta acgtctggat aaacagaatc gacgtcaggg taaacggcag
    10861 cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga cgttatacag
    10921 atgccacatt cccaccatcg acacgcccag cgccgcgacc accagcagct tggtcagcac
    10981 catgccggtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg ccaccagaat
    11041 gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg tcacgccgaa
    11101 ggtatgcaga taggaagcac tatcgttggt gatggcggtg gaatagacga acatccaaat
    11161 caccagcatt acgaaataga gcgcacctaa caggatgccc cagtttttac ctaaataacc
    11221 gctaatgaca ctcgggtaat ctttacattc tggtgattct gccagcgtat taataaacaa
    11281 ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa atacccacaa
    11341 tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat
    11401 actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc gccactcgct
    11461 ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc ctttattcga
    11521 tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt ttccgagccg
    11581 catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa agaaatgata
    11641 cgcgcgaggt cagagaaaat cttcacaatc aagactttga aataacggag atggataaga
    11701 attttctact aaattaatcg caagagaata ttcattctct atttgcggca gatcacaaaa
    11761 aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag ccgcagacca
    11821 ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata tctttgccgg
    11881 tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag gtgcggcgca
    11941 tcgccatccg cgcggcgttt actgctttca cggcattaat ggcattacgt tcaatgcacg
    12001 ggatttgtac ctgtccggca accggatcgc aggtcagccc aaggttatgc tccatcgcga
    12061 tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt aaccctgccg
    12121 ccgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg ccagagatgg
    12181 aggcgttcat tttatacagc gcgccaatag cccccgctgc cagaaaatag cgggcaattg
    12241 accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc ggaataatgc
    12301 cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt tcttcactaa
    12361 ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta gagatgttat
    12421 cgctggaaac cagctgacga cgcagcgcta cggcacgacg cggcacattg agcggaccag
    12481 gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact tgccagatac
    12541 gggcaaaccc tgcgtcaatt tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca
    12601 ggccagatat agacaggccg ttgtaatcac acattttcag cagttcacct gctgagtgga
    12661 aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa
    12721 taaacccgcc gccgacagag taataggttt tacttaatag cgcttcctgt cccttccagg
    12781 cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag ataatgttct
    12841 ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc ccgctgcgcg
    12901 ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga ctgtttcctg
    12961 ccagtcccat gataatggcg acatccgtgg catggccttt gcccgtcagt gacaacgacc
    13021 catacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt tccagccgat
    13081 caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag ggaccaatcc
    13141 caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc cgttcagtga
    13201 agtggagcgg aacgaccttg cgaccgtccc gctcacgagg ctttacgcac tacgtactgc
    13261 gatagcttca atttccagct tcacatcttt cggcaaacgc gcaacctgca cacagctccg
    13321 ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcat tgatggtggc
    13381 aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca cgctcagccc
    13441 ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc
    13501 agccgggatc tcaccggtct gcgggcaaac cggaatttgc ccggaggtga agaccatgct
    13561 gcctaaatca acgccctgaa cataagggcc gattgcgcct ggggcacgtt gcgtttcgat
    13621 aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat
    13681 cctgttgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag acacggattg
    13741 tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc cgattcatta
    13801 cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa tacccatcca
    13861 gcaggccgac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc gcaggaacga
    13921 ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg
    13981 tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg cccggcgcga
    14041 acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg tacaccacgt
    14101 tagaagtgat agtcagaatc gactgggtag tgacggcgtt gcgataggtt ggcagcgctt
    14161 taattttctt cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt
    14221 tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt
    14281 cacggattgg tttcacgcgg gcatatttga tggcagacag ggagtccgtc gccaccgaca
    14341 ggcccgcgat gccgcatgcc atagtgcgat agacatcacg atcgtgcagc gccatcagcg
    14401 aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact
    14461 gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg taatccagca
    14521 cgtcgtccat cagcggtgct gttttcggcc cgacctgaat cttcagcttc tcgtccaccc
    14581 cgccgttaat tgcgtagagc agcgttttcg ccaggttagc gcgtgcacca aagaactgca
    14641 tttgcttacc aatcaccatt gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga
    14701 agtcagtacg catcagatcg tcattttcat actgcaagga agaggtgacg atcgacacct
    14761 gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg gtcaggttag
    14821 gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca
    14881 ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag attgggtcgc
    14941 cggagaacag cgaatcaaat tccggtgtac gcaggaagcg taccatacgg atcttcatga
    15001 tgaagtgatc gatcagttcc tgtgcctgct gctcattgag tacgccagct ttaaagtcgc
    15061 gctcaatgta gatatcgagg aacgatgccg tgcggcccag cgacatcgcg ccgccatttt
    15121 gcgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc tgcgcattct
    15181 gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc tgcaacagcg
    15241 catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga tcctcgcctt
    15301 tttccagacg agactggaga tcggcaaatt gcagttcgcg ttcacgtacc agataactga
    15361 tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacggcca tagccatctg
    15421 gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag taaacatcaa
    15481 atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat tcactgtcca
    15541 tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg aacgggtgta
    15601 gcgcacgttt caacggcgca tccgtttgca ggccaacaat tttttccagc ggctggttaa
    15661 tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg
    15721 cgtgggttgc gttttcgata cggatacctt ccattacttt ttcccacaat tccgtggtgg
    15781 caggcgtcgc ttcggcgagg aaagattcat cgccttcata cggtgtatag ttatgttgaa
    15841 taaaatcgcg gacattaatt tcgtttttcc agtccgtacc tttaaagcca agccatgcgt
    15901 cggcgtacag cttatcgctg gtatcaatat ctaccttcat gaaaaataat ctctctacaa
    15961 tacttcaact aaattatgca aattctgcgg gcgcgttaac tttgcctaaa tgaatggcat
    16021 ccaaagcaat cattttttct tcgttagtcg gaataacagc gcaaatgaca cgcgcatttt
    16081 cactggaaac aattcgctca ccacaggagt tagagcgatt attcatttct gtatcaatct
    16141 ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaagctt gaattctctc
    16201 ctattccgcc ggtgaatata attccatcca ggcgacgtaa tgaagctgcg tgtccggcaa
    16261 tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt
    16321 catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata cctaataatc
    16381 ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt tggctggcga
    16441 cccaggacat cgcaccaaag tcgacatcgc cactgcgggt acccatcatc aagccttcca
    16501 gcggcgtcat tcccattgaa gtatcaacac tctgaccgtt gcgaaccgcg cagattgacg
    16561 cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga ttcagcagcg
    16621 aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac
    16681 ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga gccatcgtct
    16741 ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat tgctgcgccg
    16801 attcaatacc acttaaattg gcgtaattat gcaggggtgc cagtggagaa acgcgacgga
    16861 tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg ccgccgtgag
    16921 cgatgcggtg gccaattaag gccacactgt catttaaatt ccgtttttcc agttcaaatg
    16981 caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc tctcccccat
    17041 ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac attaatactt
    17101 cacagtcgct ggcatcgagt acggaaaact taatcgaaga cgaaccacag ttaataacca
    17161 aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta aaacagtttg
    17221 tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt atccagacga
    17281 ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa caggcacagc
    17341 agggatgcga taatcggtgc gcccatggct tcaataaggt cgaggatgtt cgggttggcg
    17401 taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgatagtgtt cagtttaccc
    17461 agcgacactt tagttttgtc gcctttataa ccgaacttca gaatcagacc attcaagcct
    17521 tccagcgtcc ccagatagtg accgaagaaa gatttgaaga tagccacgag tgcgatgatg
    17581 gaagccgcat attccagtgt aatcgcgaac gttgttttgg taccggtcat ggacgcaaag
    17641 tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc catgttggcc
    17701 ggagacagcg taaacaggca gctaaaggca aagaacatca ccactgcaac catcagcatg
    17761 ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc gaagtctttc
    17821 tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa gttaaaggag
    17881 aaaaccatga tggaaatccc cagccagaca gtgatcagga taccgtcatg accggttaac
    17941 gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg gatcagcgac
    18001 agagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat cactttaacc
    18061 atcagatcct taccaaacca gatgacgaaa gccatcagca gcaacaggaa cagcgccaca
    18121 aagccgcgat tcagcggtgc aaatccgagc tggttttccc agaacgtcat aaaggtattg
    18181 gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta caggaacgtg
    18241 ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat gttgccggaa
    18301 gggttagagc cagaaagaca cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg
    18361 tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc gcggataggg
    18421 aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca tgtggtatct
    18481 gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga agtactcata
    18541 tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa
    18601 agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat atattggtct
    18661 aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt gacgacttta
    18721 ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac cagcacgatg
    18781 tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcggga gacatcacaa
    18841 ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg gaaagaagcc
    18901 gccatgccgt gaacgttttc agactgtacg ccaataacac gaatggtcgg gttaatagat
    18961 ttaattgcca ccgcaatacc agcaattaaa ccgccaccac caattggcac aatcacgtta
    19021 tcgacatcat agagatcttc cataatttcc agaccaatcg ttccctggcc agcaatcact
    19081 ttcggatcat cgtaaggtgg gataaaaata cggccttcca tttcgacaat ttcgctcact
    19141 ttagcgatag tgtcgttgaa attatcacca tgcagaacga cttctgcgga gtagtcgcac
    19201 gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc gtcgataccc
    19261 agcatcgcgc aggagagcga aaccccttgc gcatggttgc ccgcagaaca ggccactaca
    19321 cctttacgtt tttccgcatc agtcagtgaa cttaatttat taaatgcgcc acgaatttta
    19381 aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt gcaacgttca
    19441 ctaaaatagt ttgagcgagg catgcctgtt ttataaattc gcccagccag tcgttgtttc
    19501 gcttcaataa tgtcatcaat agcaaccggc agatcgtatg taatatgcat tataaaacct
    19561 cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaaaaca gacaggtgga
    19621 ttatttatgt ttaaataaaa acaaataact aaccaacttc tattaattgc cttcgtctac
    19681 acccattata agatgaatac tctttggcta attccaccaa aaccgatgct gcttttttaa
    19741 tacgataatt tttcgaccat accacggcat attgtgccac aggtaatgtt tcttcaaccg
    19801 gaatagtaat aaattgattt gaaccaaaag gtgacgtcat atcacaagga attacagtta
    19861 agaaatcagc attgagaaca agattataaa ttgtcacgac tgagtcggtt ttaacgatgt
    19921 tttcaatact gatgccattt ctttgtaacg tagtaagaag ctcgctgtag taccccatat
    19981 tggtttgtgg caacacccac tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc
    20041 atgttcggga cttactggct accagcacaa actcggactc gaacagcggc tcaacatgta
    20101 aatcctgaag cttcatttct gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc
    20161 ggattgccgg taagaacgaa gacagttgcg cttcatacat agaaacctgc gctttcggga
    20221 acacctcttt gaacttgttg atcatccctg acataaaagt aaaaccaatc aatgaaggaa
    20281 aaccaaatga gacttccacc accgcctcag aagacatacc gcttatctca ttaaccatat
    20341 ttttcatttc acgggtaatg gattcggaac gggagagtaa caattgaccg gcaggtgtta
    20401 atgttacacc ggtatttttc cgcaccacta attccacacc aaaataatct tcaatatcgt
    20461 taatgatttt actgacggcc ggttgagtta accctaattc ttttgcagcc gagccgatag
    20521 agccacttct aatgacttcc tgaaagacta ccaggtgctg cgttttcgga agaagaatag
    20581 tgctcataac gacctacgtt aattacctca ttgacggcat gaagtgtatc aaaatgaaat
    20641 gaacaggata tgtgcgacca ctcacaaatt aactttcaat actttccatg gtatcgttac
    20701 gcaaattaaa tacctttaaa tatcaacaag ttaaagtata aaaatcagca taaaccctga
    20761 tttttgtcaa
//
LOCUS       DAZKGS010000072        20378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKGS010000072 DAZKGS010000000
VERSION     DAZKGS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHOQY_002673"
     CDS             <1..882
                     /locus_tag="ACHOQY_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9678748.1"
                     /translation="LLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQR
                     DDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKV
                     RTTVSRKAVSVGDRVNRQFVAERPDQLWVADFTYVSTWQGFVCVAFIIDVFAGCIVGW
                     RVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTERLKEAKLLASTGST
                     GDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHIPPA
                     EAEKAYYASIRNDDLAA"
     misc_feature    2..66
                     /locus_tag="ACHOQY_002673"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            1055..3265
                     /gene="katP"
                     /locus_tag="ACHOQY_002674"
     CDS             1055..3265
                     /gene="katP"
                     /locus_tag="ACHOQY_002674"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFP9678749.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            3309..3698
                     /locus_tag="ACHOQY_002675"
     CDS             3309..3698
                     /locus_tag="ACHOQY_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9678750.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            3812..4579
                     /locus_tag="ACHOQY_002676"
                     /pseudo
     CDS             3812..4579
                     /locus_tag="ACHOQY_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    4092..4202
                     /locus_tag="ACHOQY_002676"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(4659..5054)
                     /locus_tag="ACHOQY_002677"
     CDS             complement(4659..5054)
                     /locus_tag="ACHOQY_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP9678751.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(5054..6013)
                     /locus_tag="ACHOQY_002678"
     CDS             complement(5054..6013)
                     /locus_tag="ACHOQY_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP9678752.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6286..7188
                     /locus_tag="ACHOQY_002679"
     CDS             6286..7188
                     /locus_tag="ACHOQY_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9678753.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            7185..7496
                     /locus_tag="ACHOQY_002680"
     CDS             7185..7496
                     /locus_tag="ACHOQY_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678754.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            7572..8255
                     /locus_tag="ACHOQY_002681"
     CDS             7572..8255
                     /locus_tag="ACHOQY_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP9678755.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            8255..8473
                     /locus_tag="ACHOQY_002682"
     CDS             8255..8473
                     /locus_tag="ACHOQY_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678756.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            8433..8919
                     /locus_tag="ACHOQY_002683"
                     /pseudo
     CDS             8433..8919
                     /locus_tag="ACHOQY_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8964..9203
                     /locus_tag="ACHOQY_002684"
                     /pseudo
     CDS             8964..9203
                     /locus_tag="ACHOQY_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9198..9446
                     /gene="psiB"
                     /locus_tag="ACHOQY_002685"
                     /pseudo
     CDS             9198..9446
                     /gene="psiB"
                     /locus_tag="ACHOQY_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            9443..10162
                     /locus_tag="ACHOQY_002686"
     CDS             9443..10162
                     /locus_tag="ACHOQY_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP9678757.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            10159..10756
                     /locus_tag="ACHOQY_002687"
                     /pseudo
     CDS             10159..10756
                     /locus_tag="ACHOQY_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11218..11718
                     /locus_tag="ACHOQY_002688"
     CDS             11218..11718
                     /locus_tag="ACHOQY_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP9678758.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(11720..12340)
                     /locus_tag="ACHOQY_002689"
     CDS             complement(11720..12340)
                     /locus_tag="ACHOQY_002689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678759.1"
                     /translation="MDLSDGAATATARGSCPGADAKVFDACGKKFCGWYIFPWTAAPC
                     CGRFSPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAW
                     FFRHRTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETT
                     KQKNASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            12446..12880
                     /locus_tag="ACHOQY_002690"
     CDS             12446..12880
                     /locus_tag="ACHOQY_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFP9678760.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12973..13239
                     /locus_tag="ACHOQY_002691"
     CDS             12973..13239
                     /locus_tag="ACHOQY_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678761.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            13304..14194
                     /locus_tag="ACHOQY_002692"
     CDS             13304..14194
                     /locus_tag="ACHOQY_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9678762.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            14194..14439
                     /locus_tag="ACHOQY_002693"
     CDS             14194..14439
                     /locus_tag="ACHOQY_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678763.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(14470..14721)
                     /locus_tag="ACHOQY_002694"
     CDS             complement(14470..14721)
                     /locus_tag="ACHOQY_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9678764.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(14755..14950)
                     /locus_tag="ACHOQY_002695"
                     /pseudo
     CDS             complement(14755..14950)
                     /locus_tag="ACHOQY_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15300..16148
                     /locus_tag="ACHOQY_002696"
     CDS             15300..16148
                     /locus_tag="ACHOQY_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9678765.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(16234..16569)
                     /locus_tag="ACHOQY_002697"
     CDS             complement(16234..16569)
                     /locus_tag="ACHOQY_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFP9678766.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            16801..17133
                     /gene="mobA"
                     /locus_tag="ACHOQY_002698"
     CDS             16801..17133
                     /gene="mobA"
                     /locus_tag="ACHOQY_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFP9678767.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            17145..19865
                     /locus_tag="ACHOQY_002699"
     CDS             17145..19865
                     /locus_tag="ACHOQY_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9678768.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(20086..>20378)
                     /locus_tag="ACHOQY_002700"
     CDS             complement(20086..>20378)
                     /locus_tag="ACHOQY_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFP9678769.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
BASE COUNT         4874 a         4874 c         5613 g         5017 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgctcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtgtacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccggaaagcc gtttccgtag gcgaccgcgt aaaccgtcag
      361 ttcgtggcag aacgtcctga ccagctgtgg gtggctgatt ttacttacgt cagcacatgg
      421 cagggcttcg tctgtgtggc gttcatcatt gatgtgtttg ccggatgtat cgtggggtgg
      481 cgagtctcat cgtctatgga aacgacattc gtgctggatg cactggagca ggcgttgtgg
      541 gcccgtcggc cgtccggcac agtccatcac agtgataaag gttctcagta tgtatcactg
      601 gcctatacgg agcgactaaa agaagcaaaa ctgctggcat caacagggag tacaggtgac
      661 tcgtatgaca acgcgatggc ggagagcatc aatggtcttt acaaagcgga ggtaatacac
      721 cgtaagagct ggaaaaaccg tgcagaagtg gaactggcca cactcacgtg ggtggactgg
      781 tataacaatc gacgattgct gggaaggctg ggtcatatcc ctccggcaga agcagaaaaa
      841 gcttattatg cttccatcag aaacgatgat ctggcagcct gagttcacag ataaaacact
      901 ctccaggaaa accggggcgg ttcagttgcg gcttggaacc ccttagtatt ttttgtctgt
      961 agtatctatc ccagcaatag gtatatcctg ttgcatcaat aaagttgact tttgtataca
     1021 acatgcgaat ttcccttaat ccggagctat tcgtatgata aaaaaaactc ttcctgttct
     1081 gattcttctg gcgctatcgg ggagcttttc taccgctgta gccgctgata aaaaagagac
     1141 tcaaaatttc tactatccag aaacactgga tttaactcct ctgagattac acagccctga
     1201 atcaaatccc tggggggctg attttgatta tgccaccaga tttcaacagc tggatatgga
     1261 ggctctgaaa aaagatatca aagatttgct gacaacttcc caggattggt ggcctgcgga
     1321 ttatggtcat tatggtcctt tctttattcg tatggcttgg cacggtgccg gaacatacag
     1381 gacatatgat ggccggggag gcgccagtgg tggtcagcaa cgttttgaac cgctgaacag
     1441 ctggccggat aacgttaatc tggataaagc ccgtcgattg ctgtggccag tcaagaaaaa
     1501 atacggctcc agtatttcct ggggagacct gatggtcctg actggtaatg ttgcccttga
     1561 atccatggga tttaaaacgc tgggatttgc tggcggaaga gaagatgact gggagtcgga
     1621 cctggtatac tgggggcctg acaacaagcc tcttgcagat aaccgggata aaaacgggaa
     1681 acttcagaaa cctcttgccg ccacgcagat gggacttatt tatgtcaatc ctgaaggccc
     1741 cggtggaaaa ccagatcctc tggcttccgc gaaagatatc agggaagctt tttcacgtat
     1801 ggccatggat gatgaggaga ctgtggccct gatcgcggga gggcatacat ttggtaaagc
     1861 acatggtgca gcgtctcctg aaaaatgtat tggcgcaggg cctgatggtg cacctgtgga
     1921 ggagcaggga ctgggatgga aaaataaatg tggtacagga aacggcaaat ataccatcac
     1981 cagtggcctg gaaggagcct ggtcgacatc gccaacccag ttcacaatgc agtatctgaa
     2041 gaatttatat aaatatgaat gggagctgca caagagtcct gccggtgctt atcagtggaa
     2101 gcctaaaaaa gcggcaaata tagttcagga cgcgcatgat ccgtctgtcc tgcatccgtt
     2161 gatgatgttt acgacggata ttgctcttaa agttgatcct gaatataaga aaataaccac
     2221 ccgtttcctg aatgatccaa aagcttttga gcaggcattc gcaagagcat ggtttaaact
     2281 gacccaccgg gatatgggac cggcagcccg atatcttggt aatgaagttc ctgcagaatc
     2341 atttatctgg caggatcctc ttcctgcggc ggattataca atgattgatg gtaaagacat
     2401 taagtcgctg aaagagcagg ttatggattt gggtatccct gcatctgagc tgataaagac
     2461 agcctgggct tcagcttcca catttcgtgt gactgattat cgtgggggaa ataatggtgc
     2521 ccgcatcagg ttacagcccg aaattaactg ggaagttaat gagcctgaaa aactgaagaa
     2581 agtactggca tccctgacct cattacagcg tgaatttaac aaaaaacagt ctgacggaaa
     2641 gaaagtgtcg ttggctgatt taattgttct ttcgggtaat gctgcaatcg aagatgcggc
     2701 cagaaaagcc ggggtggaac ttgagattcc ctttactccg ggaagaactg acgcctctca
     2761 ggagcagacg gatgttgcct cattcagtgt actggagccg acagcagatg gattcagaaa
     2821 ttattactca aaaagcagaa gtcatatatc gccggttgaa agcctcattg ataaagccag
     2881 tcagctggat ctcaccgttc ctgaaatgac ggcattactg ggtggtctgc gggtaatgga
     2941 tattaataca aataattctt cgttgggagt gtttaccgat acccctggtg ttctggataa
     3001 caagtttttt gttaatctgc tggatatgtc aacacgatgg agtaaagcag ataaagaaga
     3061 tacatacaat ggattcgatc gtaaaacggg agcattaaaa tggaaagcat cctctgttga
     3121 tttaatcttc agttcaaatc ctgaattacg tgcggtggca gaagtatatg cctcggatga
     3181 tgcgagaaat aagtttattc atgattttgt taaatcgtgg aataaagtta tgaatagcga
     3241 tcggtttgat ttaaacaata aatgataaaa ctgatgaggg agggcacctc cctcatgaag
     3301 gagaaattat gcagaaaagc aataaagtta taatcagctg tggtatggca ttatattttt
     3361 tgtgtggggg gattacagtg gcagaagaac ttgatgaaag catgcaagtt cttgcaagga
     3421 atatgagtgt acttcagtca tcaatggata aaaaggagct tcttgaagct cttgacagaa
     3481 tggatgatgc tgtagatgag tcaatgaagc gtcttcctga gagcatttca ccggctgatg
     3541 aagaagggaa gaatgactat ataaaagaac ttcaaaagct tgaaaaagag attatgctgg
     3601 cgaaacaaaa agtcttatcg ggacagttat cagatatacc cgattcggta aacaagatga
     3661 ataaaatcag agttgagggg catgataaat tcagataaat cagttggttg attcctgcgg
     3721 gacgaataca tacgtcagga acataacttt atggcagtga accgccccgg gaatcctgga
     3781 gactaaactt cctgagaaag aggtaaacag gatgactaaa aatactcgtt tttcccccga
     3841 agtccgtcaa cgggcgattc atatggttct ggaaagtcag ggcgaatatg actcacaatg
     3901 ggcggcaatt tgttccattg cccaaagatt ggctgtacac cggagactct gcgtgtctgg
     3961 ggacgccagc atgagcggga taccgggagc ggtgatggtg ggctcaccag cgctgaacgt
     4021 cagcgtctga aagagctgga acgtgaaaac cgtgaactgc gccgcagtaa cgatatcctt
     4081 cgccaggctt ccgcttattt tgcgaaggcc gagttcgacc gcctctggaa aaaataatgc
     4141 cactgctgct gcgtgagcag tacggggtcg gaccggtatg cagtgaactg catattgccc
     4201 cgtcaacgta ttaccactgt cagcaacagc ggtatcatcc ttataaacgt agtgcctgtg
     4261 cacagcgcga tgactggctg aagaaagaga tactgcgctt atacgatgag aatcatcagg
     4321 tatacggtgg cgtaaagtct gacgtcagtt gttacaggaa ggtatcaggg tggccagatg
     4381 tatagtggcg cgcctcatgg cggttatggg acttgccggt gttctccggg gtaaaaaggt
     4441 ccgcactacc gtcagccgga aagctgttgc cgcaggcgac agcgtaaacc gccagttcgt
     4501 ggcagaacgt cctgaccagc tgtgggtggc ggattttact tacgtcagca catggcaggg
     4561 cttcgtctgt atggcgttcg atttagggca ctaaaaccgc tgctggtccc tcataataaa
     4621 agaagagcag gtaatagact taacctgctc ttcttttatt atttttttaa catagaaaga
     4681 ttgcgtctgg tttgttcttc gaaatcatct tttgaagtga tgctctctga tttgttttct
     4741 gaagatgccc ctatgatgtt ttttaacaca cttatatcaa ttgatgtaga tgtgtatcct
     4801 gttgtttgct ctataagttt caccagattt tctgcagtga ttttctcact gaaaaaacca
     4861 ctgattagcg ttaacagtcg ggggtcaaca gaataaagag ctgctccgca aataagagat
     4921 tgtctgtaga agtctccccg catttgtatc gggagagagt ctattgtttg ttgtgtcagg
     4981 ttatcctgcg gataaagtgt cgggtgcagg tatgccacaa ctttctttct ttttttcgga
     5041 tcactgcttt ccattacgca tgcaccattg ctttcaccaa tgcaaactga ggttcatcca
     5101 ccttgttcac tttcagattc gggaaatatt gctttatgtg agggtaaata agctcggctc
     5161 ctccgccagt gagatagatt cggttcattc ctgagaatgt tgaaatttcc tgcctgatgc
     5221 tttctgccag agatttcact tccctgtcta ttacatgagt aatgtttttt atttcagcta
     5281 catcatttat taatgtctgg agatattcat tatcatgtct gttttttatt aactcatcag
     5341 caataaaata attacatggt gtggaggctt tcgtcagcgc attcatgacg gtatgagtga
     5401 tgcgggatgt cccgatttct gaatacccac ggatttcaga aatgccttca aatgcaccga
     5461 gaattaaacc acaatccaga gtggttcctc caagatcgat aatcaggctg cgttcaagtt
     5521 tgtttacttt atcttttttt agtgattcaa atgcagctgg caaagattca ggcattacat
     5581 taactttttt aattttaaat gtttcccctt tattcagaga tatctcacgg agaacatttg
     5641 ccttttttct ttctattctt tcttcatttg gttgattgtc attatcaaag aattcggtta
     5701 caggtaatgt cactgtcagt tctacatcct gtggctgaag gccggatgtc agcagtgcat
     5761 gatgtatggc aagcaaattg gtggtgctgt attgataact gacatgtgtt gtacctatga
     5821 cagcagtact gcccaaatca taggtgtatt tttttccatc cacgacgtaa ttgtaaactt
     5881 tgccggcatt aaacagtccc gggttccagc cttctttaaa gctgtttcct gacagactgg
     5941 ttcttacctc gccatcttca agccaggcca gttttacgtt agttgaacca tcatcgcatg
     6001 atacttttag cattttttct tgcctcgttt tgagttttta tttatctcaa attgagcatc
     6061 ctttatctag atgcatcttt ttgagttttt tgttgttcct tattgtgtct cattttgggt
     6121 tcatgtcaag cgatgaggct cttttagagt ctctttgcat tcttgttgtg attcagtttg
     6181 agtctctatc gatttctcat tttgcgtttc gaactgagac ggaaactact taaccaggcc
     6241 gcatgtgaga ggagaattcc catgccaaac tggtgctcaa atcgtatgca tttttctggt
     6301 gaaccggcac agattgctga gattaaacga ctggccagcg gtgccgtcac accgctttat
     6361 cgccgcgcca caaatgaagg tattcagctg tttctggccg gaagtgccgg acttctgcag
     6421 accactgaag atgtgcggtt tgaaccatgc cccggactga cggctgccgg gcgtggcgtt
     6481 gtatcgccgg agaatatcgc gttcacccgc tggctgacat acctgcagaa cggtgtactg
     6541 ctggatgagc aaaactgcct gatgctgcat gaactctggc tgcagagcgg tactggacag
     6601 cgtcgctggg aaggattacc ggacgatgtc agggatacca tcaccgcact tttcaccgca
     6661 aaaagaggtg actggtgtgg cttctggagt aacgaggatg tatcggtgtg gtggaaccgt
     6721 ctgtgtgaca acgtactgcc ggaaaaaacc atgccgtttg acctgctgac ggttctgccg
     6781 acccgcctgg atgttgaagt gaatggcttt aacggtggtg ttctgaacgg tgttccttct
     6841 gcatatcact ggtatacgga acagtatggc gtgaagtggc ctgtggggta tgaggtgaat
     6901 atcagtagtc agggagacaa cttcattcag gttgatttcg acacgccgtg gtatcagccg
     6961 gaaagcgacg ttattgcaga attaagccgc cgtttcagct gcacactgga gcactggtat
     7021 gccgaacagg gctgcaattt ctgtggctgg cagcgatatg agcgtggcga gcttgttgat
     7081 gtgctgtggg gggagctgga atggtcacat ccgacagatg atgacgagtt gccggaagtc
     7141 accggacctg cgtggatagt cgacaaggtg gcgcattatg gcggatgaag tatgacggag
     7201 acgggcgggc aactgcccgt ttcttttccg gtaaaggatg tcgccgtgct cctcttttta
     7261 ctgcgccggc tgacgcggcg cggcataaac gctgtctgtg gtcagtgtcc cgcgtccggc
     7321 gggcacggga cgggcgcttt taccgctccc ggctggcgtc ggtgacagtg tacgccagcc
     7381 cgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg cggcatagcc ttgccgtcaa
     7441 aaagacgaag gctgcggtat tccacggtcg gcctgacccg ttaccggacg cggtgaacag
     7501 cccacaggca gcgggtaacg ggcaccacag gggtgccctc ccggtgccct ctgtaagaga
     7561 aggagttttg tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg gcccgtattc
     7621 ctgataacgc cattgatttc atactgactg acccgccgta cctcgtcggt ttccgtgacc
     7681 gttccgggcg caccattgcc ggtgacgtta atgatgactg gctgcaacct gccagcaatg
     7741 agatgtaccg cgtactcaaa aaagacgcgc tgatggtcag cttctacggc tggcaccgta
     7801 ttgaccgctt tatggcggtc tggatacgcg ccggattcag cgtggcaggg cacctcgtgt
     7861 tcaccaaaaa ctacacatcc aaatctgcgt atgtcgcgta ccgccacgaa tgcgcttaca
     7921 tcctggcaaa gggcagtccg gcccggcccc gtaacccgct gccggatgtg ctgggctgga
     7981 aatattcggg caaccgccat cacccgacgg aaaagcccgt taccagcctg caaccgctga
     8041 ttgagagctt cacacacccg aactcaattg tgctggaccc gtttgcaggc agcggttcaa
     8101 cctgcgtggc cgcccttcag tccggacgcc ggtatattgg tatcgagctg cttgaacagt
     8161 atcaccgtgc cggacagcaa cgccttgccg ccgtgcaacg ggccatgcag cagggggccg
     8221 caaatgataa caggtttgaa ccggaggcag catgatgaat tacagcggat ttgaagatat
     8281 gcgtgaacag gtggctgaac tggccaacac catgtgcgac ctgcgcacaa ccatgaatga
     8341 gatggagcag cggtacagcg atgcagacac cctgccggaa cgtctggtgc gtcagacgct
     8401 ctttcgtgcc agccacctgt ttatgaaggc atataacgaa atactggaac tggattactg
     8461 cttcaaagat tgaggggaaa gagtatgtac ggaacatgcg aaacgctctg ccgggcgctg
     8521 gcagcaaagt atccgggaga cacgccgttg atgctggtta tctggtcccc ggaagagatt
     8581 caggccctcg ctgacggaat ggatatttcc ctggccgatc atgaaatcag gaccatcctg
     8641 gcgcgcctgg aggacatccc ggaagaccag cggactgagt ccggtatttc ttcctccgcg
     8701 gcgatggaga ttatcagcaa cgtgagcgaa aaccgccagg tgaccgtccc tgctgaactg
     8761 ctggcgtccc tgattcagac cgctgaacag gcattgtgga agcgtgaatg ggccgcccgt
     8821 gattacggcc tcgccgtccc ggaatgcgtt acccgccgtc aggcggtggt taatcaggcc
     8881 cgcatcctgc tgaaaaacaa cacgcacgaa aacgactgat gttatcgccg cctccgggcg
     8941 gcgattcagg gagaccatga atcatgaata aaacgctgaa tgcactgatt tgtcgtcatg
     9001 cccgcgacct gctgctggcg cagggctggc cggaagagac ggatgttgac cagcgtaacc
     9061 tgaagtatcc gggctggatc agcatttacg tgcggctgga tgcgccccgg ctggcgacgt
     9121 tactcgttaa ccgccacgat ggcgtactgc cgccgcacct ggcctccgcc attcagaaac
     9181 tgaccggaac cggggcggaa cttggtttca cgccagccca cgaacgtttc cacctggcat
     9241 tatgttcgcc gggagacgtc tctcagctct ggatgctggt tctggtgaat tgtggtggac
     9301 agcctttcgc cgtcgttcag gtgcaacata tcttcacgcc tgtcgctatc agtcacacgc
     9361 tggcgcttgc cgcgacactt gatgcgcagg gatacagtgt taacgacatc atccatatcc
     9421 tgatggcaga aggaggtcag gcatgagcgc acgttcacgg gcactgattc ccctcagcgc
     9481 agaacaacag gccgccatgc aggctgtggc tgtcacagaa caacgtcgtc gtcagggacg
     9541 cacactttca gcatggcctt atgccagcgc tttctttcgc tgcctgaatg gcagtcgccg
     9601 gatttcgctg accgatctcc gcttttttgc ccctgcgctg acgaaggagg aatttcatgg
     9661 caaccgtctc ctgtggctgg ctgccgtgga taaactgatt gaaagttttg gtgaagtctg
     9721 tgttcttccc ctgccatccg atgcggggca tcgtctgttc ccgtccgttc cttttcgtga
     9781 aggtgagcgt cgtcgtcaga aaaccacgct gacagagcag aaatacagcc gccagcggga
     9841 acgtgaggca gaacgacggg aactggaata ccagacatgt tttgctcagg cgcagattga
     9901 ccttgcgttt catactcccg ccacggtcgg aagctggttg tcccgctggt ctggtgttgt
     9961 tgaggagcat gatctggaaa cgattttctg ggggtggtgc gggcgttttc catcactgtc
    10021 atcatttgac cggtttttct ggcaggagga accactctgg cggctgattt ttgaagccgg
    10081 tgaggccggt cgtggtgcac cggtacaggt acgtgcactt gagcagtgga tgatcccgaa
    10141 caagctggag aacgcaatat gatgaaatca gacgaacaat atcaggttcc cgtgtggatg
    10201 cgacctctgt tgccgttgct ctgcaatacc ggtggtaatg atccggagga acttctgaat
    10261 gatacagaaa ccaccgccag tgcgaatatt gtccgttatg tactgatagt tgcagtgcgg
    10321 tcgcagattg gatctgctgc agctactgta caggaaagga ctgttgcgca cagagatacc
    10381 aggtggcttt tcaccggaag aagcgcagga actcctggat aatctggtgc gcagccatat
    10441 cagcaaggcg ctgtcgggtg agcgaatggc agcccgtgac agatatgccg agctggcctg
    10501 gattcgccag caactggtgg atgccgcctg gtttgtccgt gccacactgg aagcgcatgg
    10561 tacgagcgtc ggaaatgaga gtccctctgc acttccagag acaatgccgg acatacagac
    10621 acgggaactg gtgatgctga tcaaacgact ggcatcttct ctgaaagcgg tgaaaccgga
    10681 cagtagcgtg gtgcgtgcgg cgcaggactg gctgtgcgac agaaaacttg tggatatcac
    10741 agatattctc cggtgaacca ggcatgtata tttttcgcag ggacacgccg atcatgatcg
    10801 tttcatatca ggggtggtct ggaggtcatg cggcgtgtcc tctgcactcg ccggaataag
    10861 gaagtcgccg gcggatccgc ttttacccgg ccatgcgggg catggccttg tgggttttca
    10921 gttatgtggc ctcagcgtcg tgtgcgggct gtgccgtgcc tccatcgtag ccgggcttct
    10981 gcgggcggga tgcaaggtaa gatgaaccgc tgcggtcacc cggtccctcc ttcccgtctg
    11041 ccgtgatttt ccggtcgttc acccgctcag atttcgcggt ctcaccctcc aactcccgtc
    11101 agccgtgcgc gtcgggctgc ggcttccctt gcgcccttgc atccctgcag gcccggcaaa
    11161 gatggaggca cggcaccgcc cggtgtcgca gaacatgtga ctatggagga ttcgggaatg
    11221 tctgttgttg cacctgctgt atacgttgga acctggcata aatacaactg tggaagcatc
    11281 gctggccgct ggtttgacct gaccacgttt gatgatgaac gcgacttttt cgccgcctgc
    11341 cgtgctcttc accaggatga agccgatcct gaactgatgt ttcaggatta tgaggggttc
    11401 ccggtgaata tggcctccga atgccatatc aactgggcct gggttgaagg cttccgccag
    11461 gcacgggatg aaggctgcga agaggcttat cgtctctggg tggatgatac cggtgagacg
    11521 gattttgaca ccttccgcga tgcctggtgg ggcgaggctg acagtgagga ggcttttgcg
    11581 gttgagttcg tcagtgatac cggcctgctg gctgacgtgc cggagacggt ggcgctctat
    11641 tttgactatg aggcgtatgc gcgggattta ttcctggact ccttcacctt tattgacggt
    11701 catgtgttcc gtcggtgatt tactcccccg ctccggcggg ggtatggcct gccgggccgc
    11761 cggaaaaccc aggcaggctt acgcctgcct gaaatacagg agggccgcgc ctgcggcgct
    11821 gcccttcctg cccgtcatgg tgtgaggcat ttttctgttt tgtcgtttct gttgttctga
    11881 tgcccggagg cggggacacc cttcggctcc cggctgcctg gcggcatccg gtcgtcggcc
    11941 cgactccgac aggcagctgc tggcgcaccc gccagtctcc gccgaacccg ctggcgcggt
    12001 ttcgggctgc cgctacggtc cgatgtctga aaaaccaggc ctgcagcgac cgttccggtc
    12061 gcgccgcccc tgtcgcgccg gaaacggcgc tccctgcaca cctgacggtg tgtaaggcca
    12121 ctgcgcctga tggcgcagtc aacccttctg ccgccacccc tgcggcctgt ttcccgctgt
    12181 ggactttccg ggggctgaag cggccacaac acggggcagc cgtccagggg aagatgtacc
    12241 agccacaaaa ctttttgccg caggcgtcaa aaacttttgc gtctgccccc ggacaactgc
    12301 cccgtgccgt ggcagtcgct gccccatcgg aaaggtccac tgcggggaga aaccccgaag
    12361 aagtggacgg cagaaggggc tttcgcccct ccgggggagc aaccggtcgg cccggcgacc
    12421 tgattcagca gaaggagacg ttttcatgac agtcagcagc acgatttccg ttttttgccg
    12481 cgacggggtg ttccgtaccg tttactgcca cctgcacggg gagccgacct ggaacggtcg
    12541 catcctgcat acccactatg ccaccggtca gctggccgaa gccctggttg aacacggaga
    12601 tatccgttgc ctcggtcccc gttgcgacaa atccgccggg catacgcttc agaacccggt
    12661 ggacggtgtg acggcttatt acggacgtga cagtggtttc cggatggaca gtgaggcgcg
    12721 tgagtaccgt tctttcatgg aggctattgc cactgaaagc actgaagagg tgcgcttcca
    12781 ttatgtgttc atcgacggct actggaaggt gatgtaccgc acgccggaag gctggaagat
    12841 gaaagcgctc gcgctggcac tgcgccgctg tccgaaatga aaaaaggcag ggcgataaac
    12901 cctgcctttc tccgccggcg cttccccgta cggaagtccg gcatctcaat cactatctat
    12961 ggagattatg ccatgagacc atccattatc ttcgcaaccg ccgagtatgt aaagcgtctg
    13021 cgtgaagagt gcctgcggga gaataaaccc ctgcaccgcc atacccgcgt cagacgtcag
    13081 gagctggcac aggatgagat taacccggac gtcctggcga tgagcggtca tatcgccaga
    13141 cgctgcagtg agcagaagcg ggtacgtatc cccgccatga aagtcagcga atggggccac
    13201 ctgctccgcg cgcttgaaat tgagcgggcc tgccactgat taacccttgc cgtctgcctg
    13261 tgcaggcggc aggatacgtt cagacatctc accaggagga accgtgagta agaagaaaaa
    13321 caccacaacg cccacgccgc atgatgccgc gttctggtcg ttcctggcga atcccgacgt
    13381 cgccagagat tttctggaac tgcatcttcc ggcggagtac cggcagttgt gcgacctgtc
    13441 cacgctgaag ctggaacccg ccacctttgt tgagccggac ctgcatcagt acgccagcga
    13501 tatcctctgg agtgtgaaaa ccaccggggg tgaagacggc tatgtttata cgctcatcga
    13561 gcaccagagc accgaaaatc tgtacatgcc tttccgcatg ttacgttaca gtgtggcggc
    13621 gatgcagaga catctggagc agcacaaaac attgccactg gtgattccgg tgctgttcta
    13681 tcatggtgag cgcagcccgt acccgtacag catgaactgg ctggactgtt ttgaggaacc
    13741 ggcgcttgcg gctaaaatat acacaaagcc gtttccgctg gttgatatca ctgtcgttga
    13801 tgacaatgaa atcatgaacc atcgccggat ggccgcactg acgctgctga tgaagcatat
    13861 ccgccatcgt gacatgatgg agctgctgga caaactcccg caggtcatgg tggaaatttc
    13921 agacgagcag gtgcgtgttc tgattcatta catcgttaac gcaggggact ctgtatcacc
    13981 ggaatttatg cgggcgctgg ctgagcgtct gccgcagcat gaggataaac tgatgactat
    14041 cgctgaacgt cttgagcaaa aaggtcgcca ggaaggagcg cttgaaaaag ccctggctat
    14101 tgcgtgccag cttcagaaaa tggggatgac gccggagcag attaagcagg ctaccggact
    14161 ttccgaagct gaactgaaga acatcactca ctgatgagaa ccacaaagcc cggcaaaatg
    14221 ccgggttttt ttgtcagcag gagcagagca aacagcatga cgattgcaga acgtcttata
    14281 cagaaaggtt tcgacgaagg ttttaagaaa ggtgcgcttg aagtggcgcg ggaagcagcc
    14341 tgccggctgc gggatatggg ctggacgccg gaacggattc aggaggcaac cggactttcc
    14401 ggtgaagaac tgaaaaagct gtttcctgat gagcagtagc ctggcattct gccaggctgt
    14461 gaatcacgct cacacattcc ctgccgtttc ccccagttca ttttccagac gcctgatgat
    14521 atctgccttg cgcatcccct gcatgtgagg cagcagatca cggactgaaa gtgcctgggt
    14581 ggtcctccag acggtgacat ccacctgttc cgcattttcc caggcgtgcc atgttctctg
    14641 tgtcacgcca aattctctgg ccgctttttc gcgtgaccag agcatgcttt ttcgccagat
    14701 tcgcagttcc cagccggtca taaaacacct ttgaaaaagt gaaaaaactt cactttattt
    14761 tatcatcata atcaccaggt cagcgcgata aataagagtg aaaaagtgaa gaaatttcac
    14821 tttttagttg taggtgtcgt gctgttttct ctgttttcac ctgtgcggga gtggtgcgcc
    14881 agtgaccacc acatgccatt gttgtgctgt cgtttttctt cagtcgcgcc ggatgcggcg
    14941 ctccctgcac acctgacggt gtgtaaggcc actgcgcctg atggcgcagc tgtctctgcc
    15001 tgtctgccgc cgcactgcgg cccgttctcc gcagacggac tttccggggg tacatacacg
    15061 accacatccc gtttcagtcg tccaggggag agatgtacca gccacaaaac tttttgccgc
    15121 aggcgtcaaa aacttttgcg tctgcccccg gacaactgcc ccgtgacgtg gtgagcgtcc
    15181 cccatcggaa aggtccactg cgggaagaac cccctgtgaa gtggacggca gaaggagctt
    15241 cgctcctccg ggaaatgacc aggctgaccc ggcagccgtg acgatgagga gacaatatga
    15301 tgaatcagac tttacccact gctgacctga atactgccgg cacgacagat attattccgt
    15361 ctatggctat cgaccgcatc atcgcgcagc gtaacgaagg tattgcactg ttcatgcagg
    15421 cgatggaatg cctggcgaca gcgcgcagga ttctgctcga tgcttccggt gacatttttc
    15481 tctacgggtt tgaagactgc gtgactgact ccgttcgatg catggataaa ccggaagaag
    15541 caaaaaagaa tatcacccgt cttgccgacc ggaaaatctg ggatcgcctg atgacttata
    15601 cgggcatgta caccttcatg agttcatgcc agcgtgatga gtggaacagc cagctgatga
    15661 gcgacacctg tcctgaaatc accctggaca atgtactggc aacattccgc catctgaatg
    15721 ccagtaagat gcagacattt gaacaggaac tgattgatat ctggcggaaa ttgtcatggg
    15781 attacagaac caataatccc tgccgtctgg gcaagagaat cattattgaa aacctgctgt
    15841 accgctggag taacgggcgt gtgacgctgg actgcagcgg acgggaggca ctggatgacc
    15901 tggtacgtcc gttttatctg ctggagggac gcaacgttcc tgacttcagg aacagcatcg
    15961 gggcgcagta tggtgaattt ctcgggaacg gcgacaatat cggtgagttg tttgaagggg
    16021 aatattttac ggtgcgtggc taccagaaag ggaccgtaca cattgtcttt aagcgtcctg
    16081 accttgttga aaaactgaat gatattattg cacggcatta tcctggtgta ttgccgccac
    16141 gagtctgatt aaacagaaaa gccctggtat ttatgccggg gctttttttt ctgtaatcat
    16201 cataatctca gaatacgatg tgacattgtc acatcaattg ttgttcatat cgatgccatc
    16261 tttcctgcct gaatctgtct ttttaggtgc tgttttagag gaagaacggc gggatggttt
    16321 tttcgattct gcttcccgaa taatgtcacg aagtgccttc tctcctactt cataccctgc
    16381 atttttgagc gtgtcacgaa catctgccag tgtataaccc tttgtcctta ccaggacaat
    16441 gatgtcatca cgaatggcat caagaaaatc acgcaatgtt tttcgttgag cggtgaggtc
    16501 cggcaattct gataaagcag cttttgccag ctggatgtca tcatctgagt aaaatttttt
    16561 agaagccatt ggcacttttt ctccgtttca tgttgaagtc tgattttatc atcaaaaact
    16621 gcctcctggc agagctgcgc cccgcctctg ccgtctgcat aagactatga tgcacaaaaa
    16681 taacaggtta taatgcctga aaaacgggac aggatgtgta atcgtataac cgcgcacgca
    16741 cggctatacg attaccacct ggtcagggct tagccccgac accccggtaa ggagcagacc
    16801 atgagtgaga agaaaacccg aagtggcagt gagaaacggc aaaagaatgt cctcattgct
    16861 gttcgttttt caccagaaga ggctgagatt gttaaagaaa aggcagaaaa aaacggactg
    16921 acagtttcaa ctctgataag aaaaactgtg ctgggaaaac agataaatgc ccgcattgac
    16981 gaagattttt tgaaagagct gatgcgtctt ggtcgcctgc agaaacatct gtttgtggaa
    17041 ggaaaacgca cgggagataa agagtatgca gaggttctcg ttgctattac agagcttgct
    17101 aatactctgc gaagagattt aatgggacgc tgagataatc gctgatgaat gccatcattc
    17161 cgaaaaaaag acgcgacgga aagtcttctt ttgaagacct gattgcgtac acctcagtgc
    17221 gggatgatgt gccggaagat gaactcaggc cagacacgga aatgaaagct gagattccgc
    17281 accggaaccg cttcagacgt cttgttgatt atgcaacacg tctgcgtaat gaaaaatttg
    17341 tttccctgat tgatgtgatg aaagacggca gtcagtgggt gaatttctat ggtgtgacct
    17401 gctttcataa ctgtttgtct cttgaatctg cggctgaaga aatgcagaaa gcagcagacc
    17461 ttgctcattt ttccaaagat gatacagatc ctgtttttca ctatatactc tcctggcctg
    17521 cgcacgaaag tccgcgttct gagcagcttt ttgactgtgt gcgtcacacc ctgaaatccc
    17581 ttgagctgtc aaaacatcag tatgtggcag ctgttcatac agacacggat aacctgcatg
    17641 tacatgttgc agtgaatcgt gtccaccctg aaaccggcta tatcaattgc cttccgtgga
    17701 gccaggaaaa actcagtcgt gcatgccgtg aacttgaaat taagcacgga ttcgcgccgg
    17761 ataatggctg ctttgtgcat gcaccgggca atcgtattgt gcgcaaaaca gcgctggtac
    17821 gtgagcgccg taatgcctgg cgacgcggga aaaagcagac gttcagggaa tatattgttc
    17881 agatgtcgat tgccgggctg cgtgaagagc ccgcacagga ctggttatct ttgcataaac
    17941 ggctggccag tgatggtctg tatatcacga tgcaggaagg ggaactggtg gtgaaggatg
    18001 gctgggacag agcgcgggaa ggtgttgcac tcagttcgtt cggaccgtta tggacagctg
    18061 aaaaactggg gcggaaactg ggggaatatc agcctgtacc gacagatatt ttcagtcagg
    18121 tggggacacc cggtcgttat gatccggaag ccataaacgt tgatatccgg ccggaaaaag
    18181 tggcagaaac ggagagtctg aaacagtacg cctgccgtca ttttgccgaa cgcttaccgg
    18241 aaatggcgag aaatggtgag ctggaaagtt gcctggatgt gcacagaact ctggcaacgg
    18301 ctggattatg gatggggatc cagcacgggc atctggtact gcatgatgga tttgataaac
    18361 aacagacacc agtacgtgca gacagtgtgt ggccgttaat gacgctggat tatatgcagg
    18421 atctggatgg tggatggcag cctgtaccga aggatatttt tactcaggtt atacccggag
    18481 aacggttccg gggacgtaat cttggtactc aggctgtcag tgattacgaa tggtatcgta
    18541 tgcggatggg gacaggtcca cagggagcaa taaagcgcga actgttttct gataaggaaa
    18601 gtctgtgggg atacggcctt attcagtgca ggagcctgat tgaagagatg atagctgacg
    18661 gggatttcag ctggcaggca tgtcatgaga tgtttgcgcg taaagggctt atgctgcaga
    18721 aacagcatca tggtcttgtg attgtggatg cgtttaatca tgagctgaca ccggtgaagg
    18781 ccagcagtat ccatcctgat ttgacgttat cccgtgccga gcctcaggcc gggccatttg
    18841 aaattgcggc tgcagatatt ttcgaacggg tgaagcctga atgtcgttac aatccggaac
    18901 ttgctgccag tgatgaagtg gaaccaggct tccggcgcga tcctgagatt cgtcgtgagc
    18961 gccgtgaagc ccgtgcagct gcgcgggaag atttgcgtgc gcgttatctt gcatggaagg
    19021 agcactggcg taaacccgac ttacgttacg gagaacggtt acgggaaatt catgctgcct
    19081 gccgtcggcg caaggcgtat atccgtgtgc agttccggga tccacaattg cgtaagctgc
    19141 attaccatat tgcggaagtg cagcgtatgc aggcgctgat caggctgaag gagagtgtga
    19201 aggaagagcg gttgtcactg attgcggagg gtaagtggta tcctttgtcc taccggcagt
    19261 gggtggaaca acaggcggct cagggagaca gggctgcagt atcgcagtta cgtggctggg
    19321 attatcgtga tcgtcgctcg cgtaataagg ataaaagacg cacaactaat gttgatcgtt
    19381 gtgtcgtact ctgcgaaccc ggaggaacgc cactttttaa taatgttgca aaacttgaag
    19441 ctcgtcttca gaaaaatgga agtgttcatt tcagggatac gcggacagga aaaaatgtct
    19501 gtacggatta cggagatcgg gttgtgttct accatcacac ggaccgaaat gaactggcag
    19561 agaagcttga tttaattgcg ccagttctgt tcagccgcaa cggaaagctt ggttttgagc
    19621 cggaaggttc ataccagcag tttaatgatg tgtttgcaga aatggtggcc tggcacaatg
    19681 cagcgggaat aaccggaaac ggacacttca ccattactcg tccggacgtg gatttgcacc
    19741 gtcaacgcag tgagcagtat taccgggaat atatcagaca gcagacccgt ttatcggtga
    19801 gccatgatga taattatacg ttacggcagg agaaaacatg ggagccacca tcacctggaa
    19861 tgtaagcccg gataagtgat ggtgcaaaga ttcagcccaa aaacggcagg aaccttcgtc
    19921 aataagggcg ggagtagtca ctgaattgag tttatacccg gttaactctg aattaagggc
    19981 cgtgtctgcg gcccttatcc agtgcgccgt aaaaccccgg acttagtgaa cagggtttta
    20041 cagtctgatt gacagatatg gtcaccagaa aatatttgta ttccttcagt atttttcgtc
    20101 agttgttggt tgtgctccac atgatttctg gggatgatca ttatttattg tctgcgattt
    20161 attattttca gcgaccggta acagtatctc cagggggagc agggtattca gaatcgcctt
    20221 cactgaaggt atatcttcag gatcagagtg ttcctcttcc ggacgattca gtgtgatata
    20281 acatacccgg cgttcttccc tggttgtgtt attgatagtg tgcacagcct ttttccacag
    20341 ttcgtttgcc cgggattcga tatctgtatc atattgcg
//
LOCUS       DAZKGS010000073        20156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKGS010000073 DAZKGS010000000
VERSION     DAZKGS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..698
                     /gene="ompL"
                     /locus_tag="ACHOQY_002701"
     CDS             6..698
                     /gene="ompL"
                     /locus_tag="ACHOQY_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP9678770.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(789..2054)
                     /gene="yihN"
                     /locus_tag="ACHOQY_002702"
     CDS             complement(789..2054)
                     /gene="yihN"
                     /locus_tag="ACHOQY_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9678771.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(2156..3136)
                     /gene="yihM"
                     /locus_tag="ACHOQY_002703"
     CDS             complement(2156..3136)
                     /gene="yihM"
                     /locus_tag="ACHOQY_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP9678772.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(3144..3854)
                     /gene="yihL"
                     /locus_tag="ACHOQY_002704"
     CDS             complement(3144..3854)
                     /gene="yihL"
                     /locus_tag="ACHOQY_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9678773.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(4071..5894)
                     /gene="typA"
                     /locus_tag="ACHOQY_002705"
     CDS             complement(4071..5894)
                     /gene="typA"
                     /locus_tag="ACHOQY_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9678774.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            6267..7676
                     /gene="glnA"
                     /locus_tag="ACHOQY_002706"
     CDS             6267..7676
                     /gene="glnA"
                     /locus_tag="ACHOQY_002706"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9678775.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            7995..9044
                     /gene="glnL"
                     /locus_tag="ACHOQY_002707"
     CDS             7995..9044
                     /gene="glnL"
                     /locus_tag="ACHOQY_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9678776.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            9056..10465
                     /gene="glnG"
                     /locus_tag="ACHOQY_002708"
     CDS             9056..10465
                     /gene="glnG"
                     /locus_tag="ACHOQY_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9678777.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            10577..10687
                     /gene="yshB"
                     /locus_tag="ACHOQY_002709"
     CDS             10577..10687
                     /gene="yshB"
                     /locus_tag="ACHOQY_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9678778.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(10877..12250)
                     /gene="hemN"
                     /locus_tag="ACHOQY_002710"
     CDS             complement(10877..12250)
                     /gene="hemN"
                     /locus_tag="ACHOQY_002710"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9678779.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(12439..12948)
                     /gene="yihI"
                     /locus_tag="ACHOQY_002711"
     CDS             complement(12439..12948)
                     /gene="yihI"
                     /locus_tag="ACHOQY_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9678780.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(13067..13233)
                     /locus_tag="ACHOQY_002712"
                     /pseudo
     CDS             complement(13067..13233)
                     /locus_tag="ACHOQY_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13530..14162
                     /gene="yihA"
                     /locus_tag="ACHOQY_002713"
     CDS             13530..14162
                     /gene="yihA"
                     /locus_tag="ACHOQY_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9678781.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(14544..17330)
                     /gene="polA"
                     /locus_tag="ACHOQY_002714"
     CDS             complement(14544..17330)
                     /gene="polA"
                     /locus_tag="ACHOQY_002714"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9678782.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            17694..18626
                     /gene="yihG"
                     /locus_tag="ACHOQY_002715"
                     /pseudo
     CDS             17694..18626
                     /gene="yihG"
                     /locus_tag="ACHOQY_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(18667..20097)
                     /gene="yihF"
                     /locus_tag="ACHOQY_002716"
     CDS             complement(18667..20097)
                     /gene="yihF"
                     /locus_tag="ACHOQY_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9678783.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         4963 a         5062 c         4818 g         5313 t
ORIGIN      
        1 ttattatgaa aaagattaat gcaataattt tattatcttc tctgacctct gcctcggtat
       61 ttgctggcgc atacgtcgaa aacagggagg catataatct tgcctccgat cagggcgagg
      121 ttatgctacg tgtgggttat aacttcgata tgggcgcggg tattatgtta accaatacct
      181 acacctttca gcgagaagat gaactaaaac atggctataa tgaaattgaa ggttggtatc
      241 ctttattcaa accaaccgat aaattaacca tccagcccgg tggcttaatt aatgataaga
      301 gtatcggttc gggtggtgca gtgtatctgg acgtcaacta taaatttgta ccatggttta
      361 atctgacagt acgaaatcgc tataaccata ataactatag ttcaacagat ttgagcgggg
      421 aactggataa taatgacacc tatgaaattg gcacctactg gaattttaaa atcaccgata
      481 aattttccta tacatttgag ccacattatt tcatacgcgt aaatgacttt aatagcagca
      541 atggaaaaga tcatcactgg gaaatcacta ataccttccg ttatcgcatt aatgaacact
      601 ggctgcctta tttcgaatta cgttggttag atcgtaatgt cgaaccgtac caccgcgagc
      661 aaaaccagat tcgtatcggg acgaagtatt tcttctgatg tgaaactgcc ggatgcggcg
      721 taaacgcctt atccggccta cggttgatac ggttttcgtt ggccggatta agcacatccg
      781 gccattaatc aagcttcagc cgccagcatc tctttcttac gttggctaat tcgcttaacc
      841 agcattacgg aaaccaccgc accacagaat gcgaagcagg ccataatgcc aaacacgatt
      901 ttgtagccaa taatccccgg atttaaatcc agaatgtagc catacagact gaagcagaac
      961 atcgccggag cgtaaccaat aaagctaccc aacgccatcg ccgcgcctgt tttattttca
     1021 gcaatttttg cttcgccgat aggtgcaaaa aatacggctc gctgtgtaaa gactatcgcg
     1081 ccaaagccca gcgtacatgc catccctaaa tagaccggca tactttcgtg cggcagcata
     1141 atcaacagta cgagcgcagc ggtactgatg ataaaggtgt agcatagata tttactcggc
     1201 gatttcagga tcttatctga aatcatacca ccaatcggtc caccaatcat tttcagacag
     1261 tattggttaa tgatgccgta agcccccacc agcgcaacgg gcaatagata gatgtttttc
     1321 aggaatggaa tgaagaatgt caggccacag taaaccgcgt aaacgaagaa gacgttaaaa
     1381 gcgataagcc agatggtttt gtctctcagc accgaggtca tactggtgtt ttgcgatgct
     1441 ccgtcttctt ttttcacctc aacggacggt gcctcttctt tgtcattcag gacaaagaaa
     1501 ataataatgc ctacggcaat cacaataagg gaatagaacc agatgcctgc tttaaaacct
     1561 aataagccac tgccaaacca ggtaaatact gccaacgcag aaaatgccac cacggtatcg
     1621 acaatgccac gccctgtttc gaagaagcca aacaaccgac cttgttgttc gctattgccc
     1681 aatcgactta ccgacttgag caagaccggc cagttcatca tgtcgcaagt aacgccaaat
     1741 agcgcccaga caaagaggat gccccaatac cccggcattg tcgtcaaata aacacccagt
     1801 aatcctgtcg caatgagtga gaatgacatg gtgtatctgc gcggtagttt atcggcaaaa
     1861 taaatagaca gaaaaaagcc cactgtggtg acaaatgagt ttaccgacat agcattacca
     1921 atttgaccat tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc
     1981 gacggtaatt tataaattgt accgccacac aatgttaata gactaaataa cgcccatttc
     2041 tttttcgtga gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa
     2101 gataccgacc attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt
     2161 ggttcagaag atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta
     2221 tctgattaat tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg
     2281 gcaatggtgt ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa
     2341 agcggaacgc cggcgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg
     2401 gctcatcatc acccaggcat ataaggtgcg ttaataacgc tttgaacggc atatcaccct
     2461 gacctgaaat acaggcttta tgacccaggc cacccggttc tttaacgatc aaggcatctt
     2521 tgatatggac ctgggtaata tgcggcgcca tcgtttttaa agcgtcgatg ggatgctcat
     2581 ttgcattgat catgttcgca aaatcaaaca gtaaggaaag agattccatc tcactttctt
     2641 tgaccagcga caccagctca tggcttttta aatcttcatg ctgttcgatc gtaaaagtca
     2701 ggccgctgtc ctgatacgtt tcccgtacat aggcaatgtc gttagcgatg atcgataata
     2761 cgtcgcgcaa attaccttca taacgtggat aaaaacgtac ggacgatgcc ccagttttca
     2821 acgcaatggc gacggcttcg tcaatagatg ccttatctga agcgctggtt tcaatatgaa
     2881 tatcaaggtt cagacggcgg gctttatccc caaaggcaga gagttcttta tcgtccatat
     2941 taccaagaga aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt
     3001 cacttgcgat atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa
     3061 aggcataagc atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt
     3121 tatttattgt caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc
     3181 caatattcaa tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt
     3241 aagatcgctg gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg
     3301 acgggtttga aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact
     3361 gcagaaaatg agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata
     3421 tatgtttcgt gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag
     3481 agttcagttg atggtgtcac tgcaatcagt ggcgcgagcg tcttgggaat atcgctggta
     3541 cggtttttct cggtaaaccc ccaggaaggc tctcgtcctt gctcaattgc ggcccgctga
     3601 aagctcgccg acaactccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta
     3661 cgatccttac ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc
     3721 atacgtttaa tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc
     3781 gtggtgatgc catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat
     3841 tgattctccg ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac
     3901 gccaaaaaag aaagcaatta acggcgtatg actttgcaga taattgtgac aaagctctct
     3961 attgacgtaa tcccagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt
     4021 aaaaaaaaac ctgccagcga tggcaggttt tttataacta aaaaacgaaa ttaatcgtct
     4081 ttcggtgcgc ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg
     4141 atagaggtcg gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc
     4201 agagtcatgc ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg
     4261 ttggtcagtt tcttaccggt caggcagttt accgtcaggt cgttagagcg gctatgaata
     4321 ccgataatct gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc
     4381 tgcagaccga acagcgcgaa cgcgaccgct ttaccctgac cgttagagat cagtacgccg
     4441 ttctgacgct gacccacttc acccggacgt acgtcgtcgt agtggctgaa ggtggagtac
     4501 agcagaccag taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg
     4561 cttgggatca cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg
     4621 tcgcctttac gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc
     4681 agagtcacgt tttcatacgg ctcttgttta cgaccgtcga tttcacggaa gataactttc
     4741 ggacgggata ccgccagttc gaaaccttca cgacgcatgt tttcgatcag aacagacagg
     4801 tgcagttcgc cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc
     4861 agcgcaacgt tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg
     4921 aacttacctt ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc
     4981 ggctcatcaa cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca
     5041 gaaatgttca gttcgccaag gcccgtgatc gcaacgatat cgccagcttc cgccagatcg
     5101 gtttcgatac gttccagacc gaggtggccc agcactttac cgactttcgc gttgcgggtt
     5161 ttgccttcgc tatcgatgat agtgacctgc tggttcggct tcactttacc gcgcttgatg
     5221 cggccaatgc cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg
     5281 aacggaccgt caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctggtacagc
     5341 ggggtcatgt cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa
     5401 gcgtaaacga tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg
     5461 aatacctgat ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca
     5521 acaatcggct tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc
     5581 gggccgtcaa atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca
     5641 acttcaccac cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc
     5701 catttgatag cggtgttttt cgcgaggatg gtaatccccc gctctttctc caaatcgttg
     5761 gagtccatca cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc
     5821 agcttgtcta ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc
     5881 aatttttcga tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt
     5941 aatcgcacta caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt
     6001 tgtgatcgct ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caacatggtg
     6061 cttaatgttt ccattgaagc actatattgg tgcaacattc acatcgtggt gcagcccttt
     6121 tgcacgatgg tgcgcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc
     6181 gctttatctt ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac
     6241 catgaccaat ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg
     6301 agcacgaagt gaagtttgtt gatttgcgct tcaccgatac taaaggtaaa gaacagcacg
     6361 tcactatccc tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg
     6421 gctcctcgat tggcggctgg aaaggcatta acgagtccga catggtgctg atgccagacg
     6481 catccaccgc agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca
     6541 tccttgaacc tggcaccctg caaggctatg accgtgaccc gcgctccatt gcgaagcgcg
     6601 ccgaagatta cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg
     6661 aattcttcct gttcgatgac atccgtttcg gatcatctat ctccggttcc cacgttgcta
     6721 tcgacgatat cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc
     6781 accgtccggc agtgaaaggc ggttacttcc cggttccacc ggtagactcg gctcaggata
     6841 ttcgttctga aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc
     6901 acgaagtagc gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa
     6961 aagctgacga aattcagatc tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta
     7021 aaaccgcgac ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc
     7081 acatgtctct gtctaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt
     7141 ctgagcaggc gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc
     7201 tggcaaaccc gaccaccaac tcttataagc gtctggtccc gggctatgaa gcaccggtaa
     7261 tgctggctta ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc
     7321 cgaaagcacg tcgtatcgaa gtacgtttcc cggatccggc agctaacccg tacctgtgct
     7381 ttgctgccct gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag
     7441 ccatggacaa aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg
     7501 caggctctct ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg
     7561 gtggcgtgtt cactgacgaa gcaattgatg cgtacatcgc tctgcgtcgc gaagaagatg
     7621 accgcgtgcg tatgactccg catccggtag agtttgagct gtactacagc gtctaatagt
     7681 tgaagttgta ctacccggcg caacaacgcc ggaggttcaa accaggccca tctggcgtaa
     7741 ttgttgcagc cagtttgaac acggacagcg cgcagaaccc ggagcgtaca ctggtacgtg
     7801 aggagttcga gcactgccca ggttcaaaat ggcaaataaa atagcctgat gggactggtt
     7861 tttagttgcc gtggaaattt cagcccatct ctgcatgggc ttttttctcc gtcaattctc
     7921 tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt gcaacctgtt
     7981 caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc ctcaactcgc
     8041 tgattaacag tattttgtta atcgatgaca acctggcgat ccattacgcc aaccctgccg
     8101 cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacaccgtta ccggaactgt
     8161 tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg gggcaaggtt
     8221 ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc gcatatcctt tctgtgacgg
     8281 cccagcgtat gccggacggc atgatcctgc tggagatggc tccgatggat aaccagcgcc
     8341 gcttaagtca ggaacagcta cagcacgccc agcaggttgc tgcccgtgat ttagtgcgcg
     8401 gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg cagctgctca
     8461 gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc gaacaggcgg
     8521 accggctgcg aaatctggtc gaccgtctgt tggggccgca gctgcccggt acgcgcgtta
     8581 ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg gaactgccgg
     8641 acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg cacgacccgg
     8701 atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg ctggggccgg
     8761 aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta cacggcgagc
     8821 gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt ccgcctcatt
     8881 tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc gggcttggct
     8941 tatccatcgc tcgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc
     9001 cagggcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga cgtttatgca
     9061 acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc ttgaacgtgc
     9121 gctcgctggg gcaggtttaa cctgtacgac gtttgagaac ggcgcagaag tgctggaggc
     9181 gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg gaatggacgg
     9241 gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca tcattatgac
     9301 cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt ttgattatct
     9361 gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta tcagtcatta
     9421 ccaggaacag cagcagccgc gtaatgttca gcttaacggc ccaacgaccg atatcatcgg
     9481 cgaagcgcca gccatgcagg acgtgttccg tattatcggt cggctttcgc gttcttctat
     9541 tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc atgccctgca
     9601 tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg aatatggcag ctatcccaaa
     9661 agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg gcgcgaatac
     9721 cattcgtcag gggcgttttg aacaggccga tggcggtaca ttattcctcg acgaaattgg
     9781 tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg gtcagtttta
     9841 ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg ccactcacca
     9901 gaatctcgaa cagcgagtgc aggaaggtaa gttccgtgag gatctgttcc accgcctgaa
     9961 cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc cccgtctggc
    10021 gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt tactgcatcc
    10081 ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc agctggaaaa
    10141 cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc aggatttgcc
    10201 cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac cggacagctg
    10261 ggcgacgctt cttgcgcagt gggcagacag agcgctgcgt tccggtcatc aaaatctgct
    10321 ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc gacatacgca
    10381 ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacaccc tgacgcgtaa
    10441 gttaaaagag ctggggatgg agtgattcac agcttgtgtg taaagattga ttattgagcg
    10501 caaattgctg gtattttacg ctttactgtt ccgataagtt cagtatgatc ttgcccggaa
    10561 aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg gcgcagttga
    10621 cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcaatga ttgggctgtt
    10681 tagttgagtt tcagattgct gatgaagtgc tctttgttca tgccggatgc ggcgtgaacg
    10741 ccttatccgg cctacaaaag caatattttc aacaggtttt gtaggcctga taagcgtagc
    10801 gcatcaggca atcttgttct cagcacattg atggcgcttt cgttttctac tttgtaaacg
    10861 aagcgccatt cactatttaa atcacccgtg agaactgctg catccgcgct ttctggcgca
    10921 gataggtatc aaagcacatg caaatgttgc ggatcagcaa gcgacctttc gccgtcacct
    10981 gtattccctt ctcatccaca tccaccagcc catcttttgc taacggggcg agcagcttga
    11041 gatcttccgc aaagtaatca gcgaagtgca aatcccacag tttctcaata ggggcgtaat
    11101 ccagacggaa gttgcagatg agcgacttaa tcacatcgcg gcgaatacag tcatcacgcg
    11161 ttagcgcaat accacgccac agcgcattgc cttgttcatc cacttgctga tagtactgct
    11221 tcaactcttt ctggttctgc gcgtagcagt cgccaatcat gctgatggcg gaaacgccca
    11281 tccccagcag atcggtatcg ccctgagtgg tgtagccctg gaagttacga tgcagcacgc
    11341 cttcacgctg ggccaccgcc agctcgtcat ccggacgggc aaagtgatcc ataccgataa
    11401 actgatagcc cgattgcgtc aggaaggcga tggtttcctg caggatatcg agtttttgct
    11461 gcggactcgg caggtcagca tctttgattt tgcgctgagc agcaaaaatg gtcggcagat
    11521 gcgcgtagtt aaagacactc agacgatcgg ggttcagctc cgccacacgt ttcagggtaa
    11581 aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat caggtcgatg ttggtggagg
    11641 taaaaccaat ctcacgcgca tggttaagca gtgcaaagat gaactcttca tcctgctcgc
    11701 ggttaaccag acgttgcact tctttgttga agtcctgcac gcccatgctc aggcgattaa
    11761 agccttcggc gcgtaaatga tcgagtacat ccagttcgat ttcccgcgga tcgacttcga
    11821 tcgaaatctc cgcatcggca ttgaactgga agttttcgcg cagcagcttc atcaggcggc
    11881 tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc ccagtgcaat tggctgacgt
    11941 gacgcccggc gaacagcggt gcacgatgga cgatttcttg ctccagcgcg tccagatact
    12001 gatcggcctt gtgctgctgg cgagtaacaa tcttattgca accgcagaag taacaaagct
    12061 tatggcagaa cgggatatgt acgtagagag ataatggacg ctcaggatag cgcgccacgg
    12121 cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc cagcgcggtc gggtacgagg
    12181 tgtatcgtgg cccggaatag ttatatttct ggatcagggc cagatcccag tcgatttgct
    12241 gtacagacat gctcactcct tccgatggcg tctctggcgg ctacggcgta ccggcggatg
    12301 cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt cgccgcgaca accgctgtag
    12361 tttaacgaat aaccacacca gataacatat aaccggaagg gttatcagca gaacgggaag
    12421 gcccacggcg gcaatccgtt agttgccccg cagcagacgc atcatgtctt cttgcttctc
    12481 gtcttcttcc tcttcttcgt catcgtcata agagaggccg agtttctgca tcaactcatc
    12541 aatacgatcc agtttagcat ccacccagga ttgctcttcg gcgctcaggg tttcgcctgc
    12601 ttccagacgt tccagcagcg catccagacg ctcatccgtt tccagtaatt ccaactccgc
    12661 ctgcggtgaa agcataggtt tctcactctt cggtttgtgc tgtttggtga ctttttcagt
    12721 cacgcccaat ggaatagggg ttttactgcc aatacgtgga tcttttggtg cgttctggcc
    12781 tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg cccggcgcat gaccacgacg
    12841 ttttttctga cgcttgcgat cacgagcttc ctgatccagc tcctcgcgtg tttttcgacg
    12901 cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat ggtttcataa tggttcgtct
    12961 tttagccgtt ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa
    13021 aaaggcgaca gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac
    13081 aatccttgca ggccgccaac acaccctgtt tttcctttag ccggaacaat ccgtgttgat
    13141 tccatttccg tttaattacg tctccggacg tttgtcttcc tgacaatcct ctgtcttcgc
    13201 ctcctggcgc tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg
    13261 ggtgctactt tacgcctttg ctttaaacaa acaatcaacc gccatcagta tttgcacatt
    13321 ttcagagttt tatataaaag caattcactg atttatatag cttatttgac atgcatcgca
    13381 gaattttctc tgctatttcg cataaagact atcggcaatg tctcacaaag cgtatggctt
    13441 ttacttacaa cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgcca
    13501 caaagctttg actgacggag acgaccgctt tgactaattt gaattatcaa cagacgcatt
    13561 ttgtgatgag tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt
    13621 ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa
    13681 gcctggctcg tacctcaaaa accccagggc gcacccagct tatcaacctg tttgaagtgg
    13741 ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga
    13801 tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga aaaacgtcag agcctgcaag
    13861 gtctggtggt gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg
    13921 agtgggcggt agacagcaat atcgccgttc tggtgctgct gaccaaagcg gacaaactgg
    13981 caagcggcgc acgtaaagcg caattgaata tggtgcgtga agctgtactg gcgtttaacg
    14041 gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca aggcgtggac aagctgcggc
    14101 agaaactgga tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat
    14161 aattttcttg ccttaatgct tgtgccggat gtggcgtatc cggcccgtaa attcaataga
    14221 ttacaattct accgttggcc tgataaacat agcgcatcag gcattacgga tcttttcttt
    14281 cgcccaataa aaaacgcccc agtcattact gactggggcg gctaaaatat tcagccaaat
    14341 ccgattacgt gaagtaaaag gtctgaaaga tagaacatct tacctctgta ccctacgcga
    14401 ctaactttac tcttttttgt tcagttcaga aagcactttt tgtaattttt tttcagttgt
    14461 tgcataggaa tttctaatag ccatcacaaa acgtgacagc ttatgttgct tacttacgaa
    14521 aaaagggcat gttcaggcga atcttagtgc gcctgatccc agttttcgcc actccccact
    14581 tccaccagca acggcacatc cagacgggta cagttttcca tcagttgatg aatctgcttc
    14641 gcgacggcat caacatcatc tttatgaact tcaaatacca gttcatcgtg tacctgcatg
    14701 atcatacgta cacgcggttg ctcagcctgt aaccacgcat caacggcaat catcgcccgt
    14761 ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct
    14821 gcacgacgag caccattgct ggatttgata tccggcagat acagacggcg tccgtccagc
    14881 gtttcaacgt agccctgctc tttcgcctga gcacgggtgc gttccatata ctccagcacg
    14941 ccagggtagc gttcgaagta aaggtccatg tacttctgcg cttctttacg tggaatgttc
    15001 aattgccgcg ccagaccgaa agcactcatg ccataaatca gaccaaagtt gatcgctttc
    15061 gcgctacggc gttgctcgct ggtgacggtt tccagtggca aactaaacac ttctgccgcc
    15121 gttgcccggt ggatatcttt tccttccgcg aatgcggtca gcaagccttt gtcacgcgaa
    15181 agatgcgcca taatgcgcag ttcaatctgc gagtagtccg ctgagacaat cacataatcc
    15241 tctggcgcaa taaacgcctg gcggatacga cgaccttctt cgttacgcac cggaatgttt
    15301 tgcaggttag gatcggttga cgataaacgt cccgttgcag ttactgcctg gtgataagag
    15361 gtatgcacac gcccggtttt cgggttgatc atcagcggca gcttgtcggt gtaggtcgat
    15421 ttcagcttcg ccagaccacg atactccaga atcacttttg gcaacggata gtccagcgcc
    15481 agttcttcca gtacctcttc cgacgttgac ggcgcgccac ccggcgtttt cttcagcggt
    15541 ttaataccct gtttttcaaa gagaatggtt tgcaactgct tggtggaaga aaggttaaat
    15601 tcttcacctg caatttcatg cgctttcttt tccagctcag ccagacgcaa ggtgagctct
    15661 tcagaatgat tgtgcagcac tttcggatcg atcttcacac cgttacgttc aatgcgtgaa
    15721 agcaccggca ccagcggcat ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt
    15781 tgcagatctg gccacatttt cagatgcaac tgcaaggtga catcagcatc ttcggcggcg
    15841 taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca gttgattttt gcctttaccc
    15901 gcaatctctt caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag gctgtccata
    15961 tcgtgacgcc cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc
    16021 ccacgcagct caatgccgta attcgccaga ataccgcgat cgtatttcag gttttgcccg
    16081 accttcagcg ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgcga
    16141 gagatttgat cgggcgcgtc aagataatca tgagcaaccg gaatatatgc cgctacgcct
    16201 ggctcgatag caaaagaaag cccgaccagg ttagcagaga tgttatcaag gctgtcggtt
    16261 tcggtatcaa atgcaaatac cggcgctttt tccagcttcg caatccacgc tttcagtgtt
    16321 tcttcatcaa ggatggtgac gtagttgtca taagaaatca ccgttgccgt cacttctggc
    16381 gcttcgtctg caacactggt ttcctgcggc ttcgcggctg gttttgcccc tttggcctgt
    16441 aaccatttgc ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacagc
    16501 cccaacaact cttccgctgc cggttgctgc acttccagtt gttcacaggt cagctccagt
    16561 tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat aagcaacttc tttgttttgc
    16621 tcgagcttcg ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc
    16681 tcggcataca gcgtatccag cccgccaaga ccttgcagca atgcctgcgc ggttttttca
    16741 ccgacgcccg gtacgccagg aatgttatcg gaggagtcac ccatcagcgc caggaaatcg
    16801 atgatcagtt ctggcggcac gccgtactta ttcaccacct cttccggtcc gaggatggta
    16861 ttcgtcatgg tattgataag cgtaatattt ggcgtcacca gctgcgccat atctttatcg
    16921 ccagtgctga tcagcaccgg acgcccggct ttttcggctt cgcgcgccag agtaccgata
    16981 acgtcgtccg cttctacgcc agaaaccgcc agcagcggca gtcccatcgc tttaaccatc
    17041 gcgtgcaggg gttcgatttg tgctcgcaga tcgttcggca ttggcgggcg atgtgatttg
    17101 taatgttcaa acagttcatc acgaaaggtt tttcccttgg cgtcaaagac caccgctgca
    17161 tgcgtcggtt tatattgcat gatcagactg cgcagcatgt tgaggacacc atacatcgca
    17221 ccggtcggct cgcctgcgct gttagtcagc gggggaaacg cgtgatatgc gcgataaaga
    17281 taagatgaac catctacaag gataagtgga ttttggggga tctgaaccat aatgtccgtg
    17341 cctgtttatc agattatggg taaaggatgc cacagacagg atgaaaacat gagtttttca
    17401 ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc tcgcaaatcg tcatgtggat
    17461 aactttgtgc ataaattatt tacatcgctt ttaatttatt atcatgcttt ttacagcatg
    17521 atatttgtct ttgtttttca tgcagttaaa aataaagcca tccctaataa ttgagataat
    17581 gggatttttt ctaatgtgga tagatatgaa ttatttttct ccttaaggat catccgttat
    17641 ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt tagcgcactg tctatggcta
    17701 accttttgaa taaattcatt atgacgagaa tactcgctgc gataaccctt ttgctgagta
    17761 tcgtattgac cattttggtc actatttttt gttccgtccc gatcatcatt gccgggattg
    17821 taaaactatt gctacctgtg ccagtcattt ggcgaaaggt ttcgcgtttt tgtgatttta
    17881 tgatgtattg ctggtgtgaa ggtctggcgg tattactgca tctgaaccca cacctgcaat
    17941 gggaagttca cgggctggaa gggttaagta agaagaactg gtatctgctt atttgtaacc
    18001 accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt tcgtaagcac attccgatga
    18061 ataaatattt cctcaagcaa cagctggcct aggtgccatt tcttggcctg gcgtgctggg
    18121 cgctggatat gccgtttatg aagcgttatt cccgcgctta tttattacgt catccggagc
    18181 gacgtggtaa agatgttgaa accactcggc gttcttgtga aaagtttcgc ctgcatccca
    18241 ccaccattgt caatttcgtt gaaggctccc gtttcacgca agaaaaacat cagcaaaccc
    18301 actccacttt tcaaaacttg ttgccaccaa aggcggcagg cattgcgatg gcgctaaatg
    18361 tactgggtaa acaattcgat aaactgctta atgttacgct gtgttatccg gacaataacc
    18421 gtcagccgtt cttcgatatg ttaagcggta aactgacgcg aattgtcgtt catgtggatt
    18481 tacagcctat tgccgatgag ttacatggcg actacatcaa tgacaaaagc tttaagcgcc
    18541 attttcagca atggctgaac tcactgtggc aggaaaaaga ccaacttctg acgtcgttga
    18601 tgtcatcaca gcggcaggac aagtaaattt tgctcgcctc tgaatgagca gaggcgagtg
    18661 agtattttaa tgaacaaaac gtccggcacg agacataaat tcttcttcgc tcatctcctg
    18721 tccgttaaaa acaactttac ccggtgtata acgcagttgc agcgaggcgg tattgttgtc
    18781 aatcgtgatt aactgaaaca tctgacctaa ggtcatcatc ccgctgattt gtttatcagc
    18841 ccgcttttgc gctttttccg catccattcc ttcagataaa ttaagctgtt ttgcggtttc
    18901 tgtgacgaca ttaagcggta acttcacatc aaaattgagc gatttaatat ctttgtttgt
    18961 ggatgatgaa gatttggctg ggtcggcaat actgatatcc agattggcat ttagttcgcc
    19021 gagtgcgttc ttccatttta ccggttgttt aatggtcggc tcactttttt gtaataacgg
    19081 taaatactct ttgaacaatg cagcattcac ttcttgcaga gcaacttcat cgtttgctaa
    19141 ctcagggtgt gcagcaagct gcttctgcat tgcaatgtta tactggatga taaattggcg
    19201 caccgctgag ggatcaatag attcagcaat caatgaccac tcaccactac cgagttgctg
    19261 attccctttt gttaacttat caagttcgta ggtcagattg acattgatat aatctttgtc
    19321 tttaacgcga tccagcatgg tttttgcaac gaaagcatcg atttgggcaa cgtcttcccc
    19381 gtgattgata atgttaatct gattaaattt cgactcgctt tcaccaaccg gaaatttctt
    19441 ttcttctaat cgcgccagag aatcaatggt aaaacttttt gccgttactt tgttcattgg
    19501 tgataattgc agaacaatat cttcgacttt gccttcaata tccaggttag acaatgaact
    19561 accatcacca ttaaaagtaa actggccctc accaaaagaa aactttccat actctgcagg
    19621 ggcaacatct aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg tcgcttccac
    19681 gggcgattta tttttcgcgg cgataaatag cggttgtgtc agttcattat tcactaagtt
    19741 cacttttgcc gctgccagtg ctgggatgac attaccatgc attaacattg tgatgggcaa
    19801 cggaccgtgc tcaacatcca catcaaatac aactttctgg cctggcttga tattgagatc
    19861 gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacgg gaattaaaaa agcctttgtc
    19921 aaagttttta taacttaacg tcatatcgta ggcatgttca cctttctttt tcgcatcgtt
    19981 aaaatcttta ataaactttt cgataccagg ctgaatttgc gtacctgtgt accatgtgcc
    20041 accaccccag attacggcta acgcaacaat aacacctgta gctgacttac gtatcatata
    20101 aatattccct gataaaacag caaaactttg aatatccact tatgctgaaa taatgt
//
LOCUS       DAZKGS010000074        19561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKGS010000074 DAZKGS010000000
VERSION     DAZKGS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..1665)
                     /gene="ybiT"
                     /locus_tag="ACHOQY_002717"
     CDS             complement(73..1665)
                     /gene="ybiT"
                     /locus_tag="ACHOQY_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9678784.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1884..2804
                     /gene="ldtB"
                     /locus_tag="ACHOQY_002718"
     CDS             1884..2804
                     /gene="ldtB"
                     /locus_tag="ACHOQY_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9678785.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2863..3981)
                     /gene="ybiR"
                     /locus_tag="ACHOQY_002719"
     CDS             complement(2863..3981)
                     /gene="ybiR"
                     /locus_tag="ACHOQY_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9678786.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3978..4445)
                     /gene="mntR"
                     /locus_tag="ACHOQY_002720"
     CDS             complement(3978..4445)
                     /gene="mntR"
                     /locus_tag="ACHOQY_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9678787.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4631..4759
                     /gene="mntS"
                     /locus_tag="ACHOQY_002721"
     CDS             4631..4759
                     /gene="mntS"
                     /locus_tag="ACHOQY_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9678788.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5031..6614
                     /gene="opgE"
                     /locus_tag="ACHOQY_002722"
     CDS             5031..6614
                     /gene="opgE"
                     /locus_tag="ACHOQY_002722"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9678789.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6663..7178)
                     /gene="ompX"
                     /locus_tag="ACHOQY_002723"
     CDS             complement(6663..7178)
                     /gene="ompX"
                     /locus_tag="ACHOQY_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9678790.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7231..7296)
                     /locus_tag="ACHOQY_002724"
     CDS             complement(7231..7296)
                     /locus_tag="ACHOQY_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678791.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7531..8418
                     /gene="rhtA"
                     /locus_tag="ACHOQY_002725"
     CDS             7531..8418
                     /gene="rhtA"
                     /locus_tag="ACHOQY_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9678792.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8717..9220
                     /gene="dps"
                     /locus_tag="ACHOQY_002726"
                     /gene_synonym="pexB"
     CDS             8717..9220
                     /gene="dps"
                     /locus_tag="ACHOQY_002726"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9678793.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9624..10370
                     /gene="glnH"
                     /locus_tag="ACHOQY_002727"
     CDS             9624..10370
                     /gene="glnH"
                     /locus_tag="ACHOQY_002727"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9678794.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10509..11168
                     /gene="glnP"
                     /locus_tag="ACHOQY_002728"
     CDS             10509..11168
                     /gene="glnP"
                     /locus_tag="ACHOQY_002728"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9678795.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11165..11887
                     /gene="glnQ"
                     /locus_tag="ACHOQY_002729"
     CDS             11165..11887
                     /gene="glnQ"
                     /locus_tag="ACHOQY_002729"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9678796.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            12004..14229
                     /gene="ybiO"
                     /locus_tag="ACHOQY_002730"
     CDS             12004..14229
                     /gene="ybiO"
                     /locus_tag="ACHOQY_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9678797.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(14226..15152)
                     /gene="rlmF"
                     /locus_tag="ACHOQY_002731"
     CDS             complement(14226..15152)
                     /gene="rlmF"
                     /locus_tag="ACHOQY_002731"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9678798.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15428..15688
                     /gene="mcbA"
                     /locus_tag="ACHOQY_002732"
     CDS             15428..15688
                     /gene="mcbA"
                     /locus_tag="ACHOQY_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9678799.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15952..18234
                     /gene="fiu"
                     /locus_tag="ACHOQY_002733"
     CDS             15952..18234
                     /gene="fiu"
                     /locus_tag="ACHOQY_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9678800.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNAYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18276..18953
                     /gene="ybiX"
                     /locus_tag="ACHOQY_002734"
     CDS             18276..18953
                     /gene="ybiX"
                     /locus_tag="ACHOQY_002734"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9678801.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19027..19293
                     /gene="ybiI"
                     /locus_tag="ACHOQY_002735"
     CDS             19027..19293
                     /gene="ybiI"
                     /locus_tag="ACHOQY_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP9678802.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYNRRGSKDSQLR"
BASE COUNT         4825 a         5125 c         4810 g         4801 t
ORIGIN      
        1 agcgcatcag gctattttgc atttgtcatc agccgccgtc accccgacag tatcggggtg
       61 acggataatc acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa
      121 gtcgatcacg cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc
      181 acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga
      241 ctcgatggat tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt
      301 ctgcatcatt aacttaccaa acagcatccg ccctttttcc ccaccggaaa gcactttagc
      361 tggctttttg atgtcgtcct ggctgaacag caaacgaccg agaatgctgc gtaccgcctg
      421 ctcgtcatcg ccttcctgct tccactgact catccattcg aacacggtca gatcattttc
      481 aaactcatat tcgtggtcct gggcgtagta accaatgcgc gcgttttcag accattttac
      541 ggtgccgctg tccggttgca gatcgcccac cagcgttttc agcagcgttg atttaccgac
      601 gccgttggta cccagtaccg caagtttttc accgacctcc agcagcaggt tgagattttt
      661 aaacagtgga ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa
      721 cagtttctta tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttcacctc
      781 ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc
      841 gttggcgcta aagcggctaa cgaaagattg caactcagca atctgcgctt tcttcttggc
      901 gttatcggcc agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt
      961 acccggataa acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt
     1021 aaggaagtga cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg
     1081 ttccagccag cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat
     1141 atccggatca gcaaacagcg cctgcgccag aagcacacgc agcttccagc caggagcaac
     1201 ttcactcatc gggccgtagt gctgttccac tggaattccc acgccaagca acaattcacc
     1261 ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat ttaacttcca gatcggccac
     1321 tttatagccg tcttcctcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt
     1381 cacttcccac aactctttat gccccatgat caccgtatcc agcacagtga actcttcaaa
     1441 ggcaaactga tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc
     1501 caaagtcggc tctaagtcgc cgccgaggat cttcataaag gtggatttac cactaccgtt
     1561 cgcgccaatc aggccgtaac ggttgccgcc gccaaatttg acggaaatgt tttcaaacaa
     1621 cggcttactg ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa
     1681 agagatatga caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt
     1741 aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt
     1801 gagctggaat gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg
     1861 ccaggcacaa aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct
     1921 tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg
     1981 ggagtcgcct ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc
     2041 tggagtattt tgccgcggag taccagatgg ggctttccaa tatgatggaa gcgaacccgg
     2101 gtgtggatac cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc
     2161 tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact
     2221 atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag
     2281 atacgcctat caactggacc accaaagttg agcgtaagaa agcaggcccg acctggacgc
     2341 cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga accgcttccg gctgtcgttc
     2401 cggcaggtcc ggataacccg atgggtctgt atgcactcta catcggtcgc ctgtatgcta
     2461 tccatggcac caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc
     2521 tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt
     2581 ttattgatga gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc
     2641 ataacccgct gtctaccacc gaagcccagt ttgaaggtca ggaaattgtg ccaattaccc
     2701 tgacgaagag cgtgcaaaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg
     2761 aagcgattaa aaaccgctcc gggatgccgg ttcgtctgaa ttaatcttca aaacttaaag
     2821 caaaaggcgg actcataatc cgcctttttt atttgccaga ccctagttgg ccgggagtat
     2881 aactaacaaa acatatccca ccaacgccgc ccacaacagc atcggtattg aatagagatg
     2941 gaaacgccac cagatgcggc gatcgttcgc catacgtagc gcaatcaaat ttgccagcga
     3001 tccgggtaat aacccaaagc cacctacgtt taccgcccat accagtaata aagacggcgg
     3061 cacatagttc agcaacaata tggtactcgg cacgttactg atcacctgcg ataaaccgat
     3121 tgccgttaac cataacccgg gttcagatag atgactcacg ttacccaaca cgccttgcaa
     3181 cgctggaagc tgggtcagta aatggacgtc gataaacatc gccataaaca ccagcagcag
     3241 cgtccagtcc acactgagca ccacgcgacg tgccagcagc gcaaagcctg ccgccacaat
     3301 caccagtccc cacagctctt gtttgaactc cagcgccgtc agaaagacga tatacagccc
     3361 cagacaactc cacaccagcc gcggtttcca ctccggtgtt tgcacccccg tatggtattg
     3421 cagcgccttt ccagggaaac aacaccagca caggagcagg agcgtcagca tcattgcgcc
     3481 agccagcggt gccatttggg caataaatcc ggcaaacgaa agaccagaac gtccccagat
     3541 aagaatattt tgcgggttgc caattggcgt cagtagcgaa ccagcgttga ctgccagcgc
     3601 ctcaaaaata atcagccgat taaccgggat ctcacacagt ctttttagcg tgatagtcag
     3661 cggaacaaca ataaacagcg cgacatcgtt ggtcagaaag gtagaaagca gcgccgccgc
     3721 cagcaccata aacatcgcca gccgacgctc cgtagcaaag cggcgcacca ttttgcgccc
     3781 cagcacatca aaataaccgc ttaactccac acctttggtc agcagcatca ggccgcttaa
     3841 ggtgatgatg gtgtgccagt cgatagcagc aggccaggat ttcggtgcaa agggcacgaa
     3901 aaagcttaat ccgataccaa caagaattaa taactgaaaa aaacgatcgc cttgcagcgt
     3961 gcgtaaaaaa ggcaggctca tttggcaccg tgtttctggg taaacaaacg aaaagcgtcg
     4021 agcgtctctt cactaacatg gtgctccatg ccttccgcgt cgcgacgggc gatttccgga
     4081 ctgacgccca acaccaacaa gaaattttcg actatctgat gtcgctcgcg gctttcctgc
     4141 gccagcttct ctccttctgc cgttaaaaac acgccacgcc aggggatcat ttcaatcagc
     4201 cccatggttg ccagccgctt aagcatttta gccaccgtcg gttgcgaaac tcccagacga
     4261 gcagccatgt ccacctgacg agcttccccc acttccctga tcaagtcaga aatcagctca
     4321 acgtaatcat caataagctc gcgccgatgc gcctctctga cctggcggaa cccttcaacg
     4381 tgctcttcca cgttcactaa ctgcgtcact ttttttgctg ttggcgtacc tgcgcgacga
     4441 ctcattgtgc ttcctcatta tggtgacgca tacatagcgt tctcatttcg tgcgcacatt
     4501 gtaaaccaga gttgcgaagg tacaaaaaat taacgtttta gcaatagcta tataatatag
     4561 cctgtgctat atctgtatgt aatgcaatca tccctcaagg atcgacggga ttagcaagtc
     4621 aggaggtctt atgaatgagt tcaagaggtg tatgcgcgtg tttagtcatt ctccctttaa
     4681 agtacggtta atgctgctct ctatgttgtg cgatatggtc aacaacaaac cgcagcaaga
     4741 taaaccttcc gataaatagc ggcgtcgcgg tacgccgctt cactcctgct ttcatgcagg
     4801 cataacgcgt tttggtctga aaaaccccac tttttgtcgg atttgcaatc cccttcgcaa
     4861 aagatttgtt cgtcagtagt tgacctgaac ggcggctcgc tctatcttct tgcagccctg
     4921 cgtatattgc ggctcgcgga tgcggacccc tttcccctct tcacgcactc ttgcaggtat
     4981 tgacccttga cgccagggta agcacatggc gtttgttacg atagtggcat atgaatttaa
     5041 ccctcaaaga atcgcttgtt acccgtagcc gggtatttag cccgtggact gcgttctact
     5101 ttttacagtc gctattaatt aacctcggct taggttaccc cttcagtttg ctctacaccg
     5161 ctgcgtttac ggctattttg cttttgctat ggcgaacatt gcctcgcgta caaaaagttc
     5221 tggtcggtat cagttcgctg gtggcggctt gttatttccc ttttgctcag gcctacggcg
     5281 cgcctaactt caatacattg ctggcattgc actccaccaa tatggaagag tcgaccgaaa
     5341 tcctgacgat ttttccgtgg tacagctaca tggtcggcat atttattttt gtgctcggcg
     5401 taatagcaat caggcgaaaa aaagagagtg aaaaagcgcg ctggaatacc ttcgacagcc
     5461 tgtgtctggt attcagtgtg gcgacatttt ttgttgctcc cgtgcaaaac ctggcctggg
     5521 gtggcgtatt taaactgaaa gatactggct atccggtatt tcgttttgct aaggatgtca
     5581 tcgtcaataa taacgaggtg attgaagagc aagaacggat ggcaaaactt tccggaatga
     5641 aagatacctg gacggtcact gccgttaagc cgaagtatca gacctatgtg gtggtgatcg
     5701 gtgaaagcgc gcgtcgcgat gccctcggtg cctttggcgg tcactgggac aataccccgt
     5761 ttgccagcag cgttaacggt ttgatatttg ctgactacat tgccgccagt ggctccacgc
     5821 agaaatcgct tggcttaacg ctcaatcgcg tagtcgatgg caaaccacag tttcaggata
     5881 actttgtcac cctggcaaat cgcgcgggct tccagacctg gtggttttcc aaccagggcc
     5941 aaatcggcga atacgatact gctatcgcca gcatcgccaa acgtgcagat gaagtgtact
     6001 tcctgaaaga aggtaatttt gaagcagata aaaacacgaa agacgaagcg ttactggata
     6061 tgaccgctca agtgctggcg caagagcact cgcaaccgca gctgattgtt ctgcatctga
     6121 tgggctcgca tccgcaggcc tgcgacagga cacaaggcaa atacgaaacc tttgtgcaat
     6181 cgaaagaaac gtcgtgctat ctctatacca tgacgcaaac ggacgattta ctgcgcaagc
     6241 tgtacgatca gttacgcaac agcggcagca gcttctctct ggtttacttt tctgaccacg
     6301 gtctggcctt taaagagcgc ggcaaagacg tgcaatacct tgcccatgat gataaatatc
     6361 agcaaaattt ccaggtgcct tttatggtca tttccagcga cgataaagcg catcgggtga
     6421 ttaaagcccg ccgctcagcc aatgacttct taggcttttt ctcgcagtgg acgggaatta
     6481 aagcgaagga aataaatatc aaatacccgt ttatatctga gaagaaagcc gggccgatat
     6541 acatcaccaa cttccagtta cagaaggtgg attacaacca tctcggaacc gatattttcg
     6601 acccgaaacc ttaaaacaaa aaatccgccc cgagaggcgg attttttata tcaccaaagt
     6661 gattagaagc ggtaaccaac accggcaatc caggtaccta cgtcaacgct acgaatacgg
     6721 ctctgctcgt aagagaagtc cagagcaacg ttttccatcg ggttgaactg cagaccagca
     6781 ccgtaggaga aaccgtagtc gctggtgtcg tgtttgtagg tcgggtattc cgtggtctgg
     6841 aatttaccat aacccacccc cactacaccg tagatgcttg cccagtcgtt aatgcggtaa
     6901 gccggaccag cagtgatgcc gtagtactgg tttttgttgt agtcaccaga gcttgcagta
     6961 cggcttttct cggtgtaagt gaaagagccg atcacaccca gcgggctgtt gtcttcttca
     7021 tagcggtatt tcaggttgaa accgcccatt ttgttcattt ggccctgagc gtcgctctgt
     7081 gcgtaaccgc cagttacggt agaagtcgca gctacggaag tacctgcggt gaaagccaga
     7141 actgcggcca gtgctgaaag acatgcaatt tttttcataa ccacctcaaa tgtgattcaa
     7201 ataagtccta agttttaaat atatcaaaaa ttaatgggaa actcttcgcg atttgtgatg
     7261 tctaacgggc catttcatgt aacagaacgt ttccatacac cgctatccat ctaaatttaa
     7321 atcacttttt cagagaactg cgtaagtatt acgcatgttt tccctgtcat tcatccagat
     7381 tattcctaat caccagacta atgattccat caatcctggc gcattttagt caaaacgggg
     7441 gaaaattttt tcaacaaatg ctcaaccatc attgggtata tccagtacac tccacgcttt
     7501 acttaagtct agatatttgt gggagaaagg atgcctggtt cattacgtaa aatgccggtc
     7561 tggttaccaa taatcatatt gctcgttgcc atggcgtcta ttcagggtgg agcctcgtta
     7621 gctaagtcac tttttcctct ggtgggcgca ccgggtgtca ctgcgctgcg tctggcatta
     7681 ggaacgctga tcctcatcgc gttctttaag ccatggcgac tgcgctttgc caaagagcaa
     7741 cggttaccgc tgttgtttta cggcgtttcg ctgggtggga tgaattatct tttttatctt
     7801 tctattcaga cagtaccgct gggtattgcg gtggcgctgg agttcaccgg accactggcg
     7861 gtggcgctgt tctcttctcg tcgcccggta gatttcgtct gggttgtgct ggcggttctt
     7921 ggtctgtggt tcctgctacc gctggggcaa gacgtttccc atgtcgattt aaccggctgt
     7981 gcgctggcac tgggggccgg ggcttgttgg gctatttaca ttttaagtgg gcaacgcgca
     8041 ggagcggaac atggccctgc gacggtggca attggttcgt tgattgcagc gttaattttc
     8101 gtgccaattg gagcgcttca ggctggtgaa gcactctggc actggtcggt tattccattg
     8161 ggtctggctg tcgctattct ctcgaccgct ctgccttatt cgctggaaat gattgccctc
     8221 acccgtttgc caacacggac atttggtacg ctgatgagca tggaaccggc gctggctgcc
     8281 gtttccggga tgattttcct cggagaaaca ctgacaccca tacagctgct ggcgctcggc
     8341 gctatcatcg ccgcttcaat ggggtctacg ctgacagtac gcaaagagag caaaataaaa
     8401 gaattagaca ttaattaaat ttacatttct gcatggttat gcataaccat gcagaatttc
     8461 tcgctacttt tcctctacac cgtctttata tatcgaatta tgcaaaagca tatttattcc
     8521 gaaaattcct ggcgagcaga taaataagaa ttgttcttat caatatatct aactcattga
     8581 atctttatta gttttgtttt tcacgcttgt taccactatt agtgtgatag gaacagccag
     8641 aatagcggaa cacatagccg gtgctatact tagtctcgtt aattactggg acataacatc
     8701 aagaggatat gaaattatga gtaccgctaa attagttaaa tcaaaagcga ccaatctgct
     8761 ttatacccgc aacgatgtct ccgacagcga gaaaaaagca acagtagagt tgctgaatcg
     8821 ccaggttatc cagtttattg atctttcttt gattaccaaa caagcgcact ggaacatgcg
     8881 cggcgctaac ttcattgccg tacatgaaat gctggatggc ttccgcaccg cactgatcga
     8941 tcatctggat accatggcag aacgtgcagt gcagctgggc ggtgtagctc tggggaccac
     9001 tcaagttatc aacagcaaaa ccccgctgaa aagttacccg ctggacatcc acaacgttca
     9061 ggatcacctg aaagaactgg ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc
     9121 gattggcgaa gcgaaagatg acgacaccgc agatatcctg accgccgcgt ctcgcgacct
     9181 ggataaattc ctgtggttta tcgagtctaa catcgaataa atccatcgct gatggtgcag
     9241 aactttagta cccgataaaa gcggcttcct gacaggaggc cgttttgttt tgcagcccac
     9301 ctcaacgcac ttatttagtg catccatctg ctatctccag ctgattaagt aaattttttg
     9361 tatccacatc atcacacaat cgttacataa agattgtttt ttcatcaggt tttacgctaa
     9421 ataatcactg tgttgagtgc acaattttag cgcaccagat tggtgcccca gaatggtgca
     9481 tcttcagggt attgccctat aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa
     9541 tgagttgcaa aactggcacg attttttcat atatgtgaat gtcacgcagg ggatcgtccc
     9601 gtggatagaa aaaaggaaat gctatgaagt ctgtattaaa agtttcactg gctgcactga
     9661 ccctggcttt tgcggtttct tctcatgccg cggataaaaa attagttgtc gcgacggata
     9721 ccgccttcgt tccgtttgaa tttaaacagg gcgataaata tgtgggcttt gacgttgatc
     9781 tgtgggctgc catcgctaaa gagctgaagc tggattacga actgaagccg atggatttca
     9841 gtgggatcat tccggcactg caaaccaaaa acgtcgatct ggcgctggcg ggcattacca
     9901 tcaccgacga gcgtaaaaaa gcgatcgatt tctctgacgg ctactacaaa agcggcctgt
     9961 tagtgatggt gaaagctaac aataacgatg tgaaaagcgt gaaagatctc gacgggaaag
    10021 tggttgctgt gaagagcggt actggctccg ttgattacgc gaaagcaaac atcaaaacta
    10081 aagatctgcg tcagttcccg aacatcgata acgcctatat ggaactgggc accaaccgcg
    10141 cagacgccgt tctgcacgat acgccaaaca ttctgtactt catcaaaacc gccggtaacg
    10201 gtcagttcaa agcggtaggt gactctctgg aagcgcagca atacggtatt gcgttcccga
    10261 aaggtagcga cgagctgcgt gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg
    10321 gaacttacaa cgaaatctac aaaaaatggt tcggtactga accgaaataa taacgctaca
    10381 cctgtaaaac gcactggcag ttccctctcc cctctgggga gaggattagg gtgaggggcg
    10441 caaacccgct ccggggccat taattaccct gaatttgatt atttacacca cggtaacagg
    10501 aacaacatat gcagtttgac tggagtgcca tctggcctgc cattccgctt ctgattgaag
    10561 gtgccaaaat gaccctgtgg atttcggtcc tcggtctggc aggcggtctg gtaatcggat
    10621 tgctggcagg ttttgcacgc accttcggtg gttggatagc caaccacgtc gcgctggtct
    10681 ttattgaagt gatccgcggc acacctatcg tcgtccaggt gatgtttatc tatttcgccc
    10741 tgccgatggc gtttaacgac ttacgcatcg acccatttac tgcggcggtg gtcaccatca
    10801 tgatcaactc cggcgcgtac attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca
    10861 aaggttttcg tgaagcagga ctggcgctcg gtctttcacg ttgggaaacc attcgctacg
    10921 tcattttacc gctggcactg cgtcgtatgc tgccgccgct gggtaaccag tggatcatca
    10981 gcattaaaga cacctcgctg tttattgtga tcggcgtggc ggaactgacc cgtcaggggc
    11041 aagaaattat tgccggtaac ttccgcgccc ttgagatctg gagcgccgtg gcggtgctct
    11101 atctgattat taccctggtg ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa
    11161 tcctgtgatt gaatttaaaa acgtctccaa gcactttggc ccaacccagg tgctgcataa
    11221 tatcgatttg aacattgccc agggcgaagt cgtggtgatt atcgggccgt ccggttccgg
    11281 taaatcgacc ctgctgcgct gcatcaacaa actggaagaa atcacctccg gcgatctgat
    11341 tgtcgatggc ctgaaggtta acgatccgaa agttgacgag cgcctgattc gccaggaagc
    11401 aggtatggtg ttccagcagt tttacctctt cccgcatctg acggcgctgg aaaacgtcat
    11461 gtttggtccg ctacgcgtgc gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga
    11521 gctgctggcg aaagtcggtc tggcagaacg tgcacatcac tacccttccg aactttctgg
    11581 tggtcaacag cagcgtgtgg cgattgcccg cgcgctggcg gtgaagccga aaatgatgct
    11641 gtttgatgaa ccgacttccg ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat
    11701 gcaggatctg gctgaagaag ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc
    11761 cgagaaagta gcttcgcggc tgatctttat cgacaaaggc cggattgcgg aagatggcga
    11821 tccgcaggta ttgatcaaga acccgccgag ccagcgcttg caggaatttt tgcagcacgt
    11881 ctcttaactt ctctgcctcc ctttcacccg aaagggaggc acaccagatt cctctcattt
    11941 aaaatcgccc ctcccccagc atctatactt atctttttgc tctgttttct cactggagga
    12001 gtcatgcggt ggatcctgtt catcctcttc tgcctgctgg gcgcacctgc ccacgcggta
    12061 tccatacccg gcgttacaac cacaacgaca acggactcaa cgactgaacc ggccccggaa
    12121 ccggatatcg aacaaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac
    12181 acctcgcgta aagaattgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa
    12241 ccggtcccga agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc
    12301 accgaagtca gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc
    12361 aatatcaccg gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc
    12421 catttttcaa tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca
    12481 ctgccgctgt atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac
    12541 tggttgcagc ttccggcgat gattatcggg gcgtttatta tcgacctgct gttactggcc
    12601 ctgacattat ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc
    12661 gctttccaac aaagtttgtt tctcaatgcc tttgccctga ttgaattttt caaagccgta
    12721 ctacgcctga ttttttgccc aaacgtggcg gagctgcgtc cgttcacgat tcaggacgag
    12781 agcgcccgtt actggagtcg tcgcctgagc tggttaagca gtttgatagg ttatggcctg
    12841 attgtggccg tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac
    12901 gtcatcatta tgctgtgcat gaccgtctgg gcgttgtacc tgatctttcg taataaaaaa
    12961 gagattaccc agcatttgct caactttgcg gagcattcgc tggccttttt cagcctgttt
    13021 atccgcgcct ttgcgctggt gtggcactgg ctggcaagcg cctattttat cgtgctgttt
    13081 ttcttttcgt tgttcgatcc gggcaacagc ctgaaattta tgatgggtgc aacggtgcgc
    13141 agcctggcga ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggcg
    13201 aaaaccatca ccctctcgcc acatactcag cgtaactatc cggagctaca aaaacggctg
    13261 aatggctggc tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta
    13321 atgttgctgt tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg
    13381 gggcagaaaa ccgtagatat cctgatccgc atcgcactca ttcttttctt ctcggcggtt
    13441 ggctggacgg tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc
    13501 ccgctaccca gcgcccgtac gcgtaccctg ctgacgctgt ttcgtaacgc gctggcggtg
    13561 attatcagta ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca
    13621 ttgctggcag gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggtg
    13681 aaagatatca ttaccggggt gtttattcag tttgaaaacg gcatgaacac tggagatttg
    13741 gtgactatcg ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg
    13801 cgccaggata ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac
    13861 ttcgtccgcg gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct
    13921 gataaagcca atcaggcact gaaagatgcg gtagcggaat tgatggaaaa cgaagaaatt
    13981 cgcgggctga ttattggtga accgaatttt gccgggattg tcggcttaag caataccgcg
    14041 tttacactgc gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc
    14101 ctcgacagcc aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact
    14161 tatcaggtgc tgcctgctcc gggcgcgacc ccggctgaac cgccgccgcc gggggagcca
    14221 acgctttaac gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag
    14281 gcaataaagc gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg
    14341 cccacgtcgg tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag
    14401 gtaaaccaca tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag
    14461 gcgacttcac caccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg
    14521 tctttgttca gccccaggtt acggcgttta cgctcactac ctgcccgcgc tgcagcggcg
    14581 gaatcgtgga atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt atggatgatg
    14641 ccgttaaaaa ttgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc
    14701 ggattagcac tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg
    14761 gtaaaacgcc agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg
    14821 ccgatatcca gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg
    14881 gcaaggtgat gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg
    14941 atatcccagt tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc
    15001 gccagcggat tggcaaagtc tacgctttgc tccccggcgg gtgtaagcgt gaggaattgc
    15061 ctgagttcag gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg
    15121 cgcggatgca accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag
    15181 atacccgcat aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta
    15241 acatggctat ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattgattt
    15301 gtcttatttt gtaaaaatat atcgttcaaa aaataagcac atctctgtca attttttgcg
    15361 tcattttcat cgcccaggcg acctacactt atcaggcact acccgttgta tttatgagga
    15421 tgtcattatg aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct
    15481 cacggcttat gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg
    15541 taccgtttcg gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa
    15601 agcgcgcgag caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat
    15661 gttcggtact gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat
    15721 gcagttttcg cctgttttgt aatcaaaaaa catcaaatat gaacgcaatg taaataaatg
    15781 tatttctttt tcgcgcaatg ggtgatagaa aatcgcgcca agtgataatg cttatcaaaa
    15841 ttattatcac ttgcacgaac actatcacga gattaacagt ggcatcgcat ccgcagagag
    15901 gctttctcgt ggcagtgaaa atttcaacat ataaaaaaag tcacctgcaa aatggaaaac
    15961 aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg tctttgtatt
    16021 ggcatcacgc ctgtggctca ggcactcgcc gccgaagggc aaactaacgc ggatgacacg
    16081 ctggttgtcg aagcatcaac gccttcgctt tatgcgccac aacaatctgc cgatccgaaa
    16141 ttctcgcgtc cggtagcgga tactacccgc acgatgacgg tgatttctga acaagtgatt
    16201 aaagatcagg gcgcaaccaa ccttaccgac gcgctcaaaa acgtccccgg cgtgggtgcg
    16261 ttttttgcgg gtgagaacgg taactccacc actggcgacg ccatttatat gcgcggtgct
    16321 gatacctcta acagtattta tattgatggc attcgcgata tcggcagcgt ctcgcgcgac
    16381 accttcaata ctgagcaggt cgaagtgatt aaagggcctt ccggcaccga ctacgggcgc
    16441 agcgcgccga cgggctcgat caatatgatc agcaagcagc cgcgcaatga ttccggcatt
    16501 gacgcctccg ccagtattgg cagcgcctgg ttccgccgcg gcacgctgga cgtcaatcag
    16561 gtcattggtg ataccactgc ggtgcgcctg aatgtgatgg gcgaaaaaac gcacgatgcc
    16621 ggacgcgaca aagtcaaaaa tgagcgttac ggcgtcgccc cttctatcgc ttttggcctt
    16681 ggtacagcga atcgtttgta tcttaattat ctgcatgtca cccagcacaa cacgccagac
    16741 ggcggcattc cgaccatcgg tttgccgggt tattctgccc catctgcggg aacagcggct
    16801 ctgaatcatt ccggaaaagt tgatactcat aacttttacg gcacggattc cgattacgac
    16861 gattcgacca ccgacaccgc caccatgcgt tttgagcacg acatcaacga taacaccacc
    16921 attcgcaata ctacccgttg gtcgcgcgta aagcaggatt acctgatgac ggcgattatg
    16981 ggcggggcgt cgaatattac tcagcccacc agcgatgtga atagctggac ctggtcacgc
    17041 acggcgaata ccaaagatgt gagtaataaa attctcacca accagaccaa cctgacctcg
    17101 acgttctata ccggttctat cggtcatgat gtcagtaccg gcgtggaatt tacccgtgaa
    17161 acgcagacta actacggcgt taatccggtg acgttacccg cggtaaatat ttatcatcct
    17221 gacagcagca ttcatcccgg cggcctgacg cgcaacggcg caaacgccaa tggtcagacg
    17281 gataccttcg caatttacgc cttcgatacg ctgcaaatca cccgtgattt tgagctgaac
    17341 ggcgggatcc gtctggataa ttatcatact gaatatgaca gtgccaccgc ctgcggcggc
    17401 agcggacgcg gtgccatcac ctgcccaact ggtgtggcaa aaggttctcc ggtcaccacc
    17461 gtcgacaccg ccaagtcggg caatctggtg aactggaaag ccggggcgct gtatcacctg
    17521 acggaaaacg gtaatgccta tattaactat gccgtttccc agcagcctcc gggcggcaac
    17581 aacttcgccc ttgcgcagtc tggcagcggt aacagtgcca accgcaccga ttttaaacca
    17641 caaaaagcca acaccagcga gattggcacc aaatggcagg ttctggataa acgcctgttg
    17701 ctcaccgccg cgctattccg tactgatatc gaaaatgaag ttgagcaaaa tgatgacgga
    17761 acttactcgc aatacggtaa gaaacgtgtc gaaggctatg agatatccgt ggccgggaat
    17821 atcacgcccg cgtggcaggt gattggcggc tatacccagc aaaaagcaac catcaaaaac
    17881 ggcaaagatg ttgcccagga tggttcctca tcgctgccgt ataccccgga gcacgccttc
    17941 accttatgga gccagtatca ggcaacagat gatatctctg ttggcgcggg cgcacgctat
    18001 atcggcagta tgcataaagg ttcagacggc gcggtgggaa cgccagcgtt taccgaaggt
    18061 tactgggtcg ccgatgccaa actggggtat cgggttaatc gcaatctcga cttccagcta
    18121 aacgtttaca acctgtttga taccgattac gtcgcctcaa tcaacaagag cggctaccgt
    18181 tatcacccgg gcgagccaag aaccttcttg ctcacagcca atatgcattt ctgattcaga
    18241 tgtggggcgc aggccccact ttttggagaa cttgtatgat gtaccacatt cccggcgtgt
    18301 tatcgccaca ggacgtcgcc cggtttcgcg aacaactgga acaagccgaa tgggtggatg
    18361 gacgcgtcac caccggcgca caaggtgcgc aagttaagaa caatcaacag gtcgacaccc
    18421 gcagcacgtt atacgccgcc ctgcaaaatg aggtgctgaa cgcggttaac caacatgctt
    18481 tattctttgc cgcggccttg ccgcgtaccc tttccacgcc gctgtttaat cgctatcaga
    18541 acaatgaaac ctatggtttc catgtggatg gcgcagtacg cagccatcca caaaacggct
    18601 ggatgcgtac tgacctttct gccacgctgt ttttaagcga tccacaaagc tacgacggcg
    18661 gcgaactggt cgttaatgac accttcggac aacatcgggt aaaactcccg gcaggcgatc
    18721 tcgtgttgta tccctccagc agcctgcatt gcgtgacacc cgtaacccgc ggcgtacgag
    18781 tggcatcatt tatgtggatc cagtcgatga tccgtgatga taaaaagcgc gccatgctgt
    18841 ttgaactgga caacaatatt cagtcgctga aaagccgcta cggcgaaagt gaagagatcc
    18901 tgtcgctgct taatctttat cataatctgc tgcgggaatg gtcggagatc tgatgcttga
    18961 actgcccagg ttcgtccctg gattagctac acttaactgt acaagcattg atatggggag
    19021 gacgatatgg catccggttg ggctaacgat gacgccgtca acgaacagat caacagtaca
    19081 attgaagatg cgattgcccg cgctcggggt gaaattccgc gtggcgaaag cctggatgaa
    19141 tgtgaagagt gcggtgcccc catcccgcaa gcccgtcggg aagccattcc tggcgtgcgc
    19201 ttatgcattc attgtcagca ggagaaaggt ttacaaaaac cagcgtatac aggatataat
    19261 cgcagaggtt cgaaagacag ccagttacgt taactacaac tggcgagatg catagcgagt
    19321 ataatttgta ttttgcgtgg cattccgtgc ctttaacgct gctgtgactg ccacgcggca
    19381 taaaacaagt ccataagtac aacaaataaa tggtttatca gtatgttaga tatcaatcaa
    19441 tttatttgaa caaggcggtc aattctcttc gattttatct ctcgtaaaaa aacgtgatac
    19501 tcatcacatc gacgaaacaa cgtcacttat acaaaaatca cctgcgagag attaattatg
    19561 a
//
LOCUS       DAZKGS010000075        19558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKGS010000075 DAZKGS010000000
VERSION     DAZKGS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(401..777)
                     /gene="rnpB"
                     /locus_tag="ACHOQY_002736"
     ncRNA           complement(401..777)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOQY_002736"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(810..1955)
                     /gene="garK"
                     /locus_tag="ACHOQY_002737"
     CDS             complement(810..1955)
                     /gene="garK"
                     /locus_tag="ACHOQY_002737"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9678803.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2052..2942)
                     /gene="garR"
                     /locus_tag="ACHOQY_002738"
     CDS             complement(2052..2942)
                     /gene="garR"
                     /locus_tag="ACHOQY_002738"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9678804.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2972..3742)
                     /gene="garL"
                     /locus_tag="ACHOQY_002739"
     CDS             complement(2972..3742)
                     /gene="garL"
                     /locus_tag="ACHOQY_002739"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9678805.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3758..5092)
                     /gene="garP"
                     /locus_tag="ACHOQY_002740"
     CDS             complement(3758..5092)
                     /gene="garP"
                     /locus_tag="ACHOQY_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9678806.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5467..7038
                     /gene="garD"
                     /locus_tag="ACHOQY_002741"
     CDS             5467..7038
                     /gene="garD"
                     /locus_tag="ACHOQY_002741"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9678807.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7187..7522
                     /gene="prlF"
                     /locus_tag="ACHOQY_002742"
     CDS             7187..7522
                     /gene="prlF"
                     /locus_tag="ACHOQY_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9678808.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7522..7986
                     /gene="yhaV"
                     /locus_tag="ACHOQY_002743"
     CDS             7522..7986
                     /gene="yhaV"
                     /locus_tag="ACHOQY_002743"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9678809.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8041..8850)
                     /gene="agaR"
                     /locus_tag="ACHOQY_002744"
     CDS             complement(8041..8850)
                     /gene="agaR"
                     /locus_tag="ACHOQY_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9678810.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9099..10379
                     /gene="kbaZ"
                     /locus_tag="ACHOQY_002745"
     CDS             9099..10379
                     /gene="kbaZ"
                     /locus_tag="ACHOQY_002745"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9678811.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10402..10875
                     /gene="agaV"
                     /locus_tag="ACHOQY_002746"
     CDS             10402..10875
                     /gene="agaV"
                     /locus_tag="ACHOQY_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9678812.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10886..11665
                     /gene="agaW"
                     /locus_tag="ACHOQY_002747"
     CDS             10886..11665
                     /gene="agaW"
                     /locus_tag="ACHOQY_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9678813.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11655..12533
                     /gene="agaE"
                     /locus_tag="ACHOQY_002748"
     CDS             11655..12533
                     /gene="agaE"
                     /locus_tag="ACHOQY_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9678814.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12551..12985
                     /gene="agaF"
                     /locus_tag="ACHOQY_002749"
     CDS             12551..12985
                     /gene="agaF"
                     /locus_tag="ACHOQY_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9678815.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12982..14115
                     /gene="nagA"
                     /locus_tag="ACHOQY_002750"
     CDS             12982..14115
                     /gene="nagA"
                     /locus_tag="ACHOQY_002750"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9678816.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14466..15620
                     /locus_tag="ACHOQY_002751"
     CDS             14466..15620
                     /locus_tag="ACHOQY_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9678817.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15633..16493
                     /gene="kbaY"
                     /locus_tag="ACHOQY_002752"
     CDS             15633..16493
                     /gene="kbaY"
                     /locus_tag="ACHOQY_002752"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9678818.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16660..17136
                     /gene="agaB"
                     /locus_tag="ACHOQY_002753"
     CDS             16660..17136
                     /gene="agaB"
                     /locus_tag="ACHOQY_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9678819.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17175..17978
                     /gene="agaC"
                     /locus_tag="ACHOQY_002754"
     CDS             17175..17978
                     /gene="agaC"
                     /locus_tag="ACHOQY_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9678820.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17968..18759
                     /gene="agaD"
                     /locus_tag="ACHOQY_002755"
     CDS             17968..18759
                     /gene="agaD"
                     /locus_tag="ACHOQY_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9678821.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18760..19515
                     /locus_tag="ACHOQY_002756"
     CDS             18760..19515
                     /locus_tag="ACHOQY_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP9678822.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4735 a         5108 c         4972 g         4743 t
ORIGIN      
        1 atatagactt aataacttct tacaaagtct attattaata ccgtagatat ttattcatct
       61 taccctgtgc accaaaagca aaaactcact gacgcgggtt tgaaactgat ggcaaccgca
      121 aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg
      181 cgtgcttttg taggcaggat aaggcgttca cgccgcatcc ggcatgaata aagcgcactc
      241 aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag
      301 ccgctttctt ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc
      361 aaaaacccgc cggtttacgc tggcgggttt tcgttaaatc aggtgaaact gaccgataag
      421 ccgggttctg tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag
      481 cctacccggg ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg
      541 gagtttaccg tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca
      601 cccttacctg atcccgcttg cgcgggccat cggcggtttg ctctctgttg cactggtcgt
      661 gggtttcccc cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc
      721 tccgcccgtc tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc
      781 gcgcagtata gagggtttgc gcgcccttgt caccccgcgt tgcgcattcc aatcgccagt
      841 gtcgcggcga tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg
      901 tccaacgtac caatgctggt cagtacgctg aagacagcat caatgccatg ctgatgtaca
      961 acgccaacat catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc
     1021 gccacgtttg cgacgccaat cggtaccttg ccgtgaatac tctggctgtc aatacgccct
     1081 tcaccggtga tcaccagcgt acaatcgtga atatgctcct ccagattcag cgccgtagtg
     1141 acgatttcaa taccactttt cagttccgca ccaagaaacg ccattagcgc cgcgcccata
     1201 ccacccgcag ctcctgcgcc ggggacatct ttcacatcaa catgcagcgc ttttttaatg
     1261 acctcggcat agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc
     1321 ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca
     1381 caagcgacgc gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca
     1441 ttcagagtat taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat
     1501 ttcgccccca gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct accgccaatg
     1561 ccgataataa tgtttgtcgc gccgctctcc agcgcctgca ggattaactc gcctgtgccg
     1621 cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc
     1681 gccgccattt caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact
     1741 ttctcgccca gcggccctgt aacccaggcg tgacgttcag aaccctgggt ggctgcaatc
     1801 atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa tagaaacgta ctgtgcatca
     1861 ggaaaaattt cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt
     1921 tctttataag agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc
     1981 cggagggcgt ttctcttatt cggcctggat tccaggcccg gattgcaata cgccatccgg
     2041 gcacgacgtc attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacgccagg
     2101 gcgctatgat ccgccgttcc taaaccatct gctcgcagtg cctgcatcat ctccataacc
     2161 gcagctgtga gcggcagttg cgcgccgacg ccgtgggaag tatccagcgc atttgccaga
     2221 tccttaatat gcagatcaat acggaagccc ggcttgaagt tgcggtccat caccatcggc
     2281 gctttggcat ccagcacggt actgcccgcc agtccaccgc gaattgcctg atacaccagg
     2341 tccgggttaa cgcccgcttt ggttgccagc gttagcgctt ctgacatcgc ggcaatattc
     2401 agcgccacaa tgacctgatt tgccagtttg gtgacgttac ctgcaccgat ttccccggta
     2461 tgcaccacgg aacccgccat cgctttcatc aaatcatagt atttgtcgaa aatagccttg
     2521 tcgccgccca ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc
     2581 ggagcatcca gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc
     2641 agcggtgcga tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg
     2701 ccattctcac ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg
     2761 acgtcgcact gttcagcgat cgctttagcc gtagacgctg tttctgcacc tgcagcaatc
     2821 acgtcagcaa tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga
     2881 aggtttttac tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc
     2941 atatcaatca tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttct
     3001 gagtggcaga gcggaagacg ccaagatcgc tgccgacagc cacaaacgtc gcgccccatt
     3061 ccagataacg acgcgcatcg gcttcgaccg gcgcgagaat accgctgggt ttgccgtgcg
     3121 cactggcacg gttaaaaatg tgctgaattg ctttttgtac atccgggtgc gaggcattgc
     3181 cgagatggcc taatgccgca gccagatcgc tggggccgac gaagataccg tctacacctt
     3241 cggtagcggc aatggcatcg acgttatcta cgccctgctg actttctatc tggaccagaa
     3301 tggtgatgtt cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggcgc
     3361 ggtgagaaac ggagacgccg cgaatgcctt ccggtgggta acgggttgat gccaccgcct
     3421 gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa gttatagaaa ccgatatcca
     3481 gaagacgctt aataattacc ggctcgttgg tcggcactcg cactactggc gcgctggcgc
     3541 tgcctttcag ggccattaac tgcggaataa acgtggagat atcgtttggc gcatgttcgc
     3601 catccagcac cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag
     3661 agagtgctga ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat
     3721 tcgggaaaac atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt
     3781 taatgtcgcc aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga
     3841 aaaccagtgc tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag
     3901 tgacaatgga ggcaacattg ccaaagacgt taaagacgcc gccacagagg ccaacaatct
     3961 ctttcggcgc ggtgtcagaa atcaccggcc agcccagagc accaaatcct ttgccaaaaa
     4021 aagccagcgc catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga
     4081 tggtggaagc cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata
     4141 aaccgcgttt gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac
     4201 acagtgccgg aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca
     4261 ccagataaat cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc
     4321 cgaaaaatac gcccaacatc atgcggttag agagcaattg cttgatgtaa tgcagtttgg
     4381 gaccgcttgc tgccgcgctg cccggctttt tgtggtccat atcgaccacc gcgccatttt
     4441 cagagataaa cttcagctct tccgcagaca tacgtgggtg atctgtcggg ttatgaatca
     4501 atttgatcca cagcgccgtc agcacaaaac caatcacccc cataacggta aagacgtgct
     4561 cccagcccca ggcgaaagtc agccagccaa gcagaggcga aaagagcgcc agcgagaaat
     4621 attgcgccga gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg
     4681 cgacaatacg ggcgttcgcc gggaatgatg gcgcttccga gaagccgagc ataaagcgca
     4741 taaagaacat ggagatccct gcccaggcca gcgggaacat atcaacaaag ccttgcagga
     4801 aagtgaatag cgaccagaaa aagaggctat aggtataaac ttttttcgag ccaaacttat
     4861 caagcagcca gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcagaaaaga
     4921 tgtaacccat cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag
     4981 acagcgttgc acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta
     5041 aatagcgggt atgcacgcct ttcttttttt cgtcaacggt gtccagaatc attttattta
     5101 cctcgggtac ttatgctgat ttttattatt atggggaagg tgttatttat gagtttcatt
     5161 tatgccgtaa cggcaatgaa ctcgggaatt agtataagca gcgcgagaat aataatcatt
     5221 gtgcaaatgc taatttaatt aatgctattt aaatattatt ttgagcatat gcacataagg
     5281 ttgcgcgcta aagcacagat ttgcgcttta ccttgccggg cggcactgca atccctgaaa
     5341 tgattgacat tgatcacatt tctgcgttta aactcctgac attcttattt cacccaatga
     5401 agtcatttat ttttaaatga gaccaggtcc tcattttaat aacccctggc tggagaatat
     5461 agtacaatgg ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt
     5521 cacgacacag ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt
     5581 tttccggatg gactggaatt aattgaacat attccccagg ggcataaagt cgcattgctg
     5641 gacattccgg ctaatggtga aattattcgt tatggtgaag tgattggtta cgccgtgcgt
     5701 gcaatcccac gcggaagctg gctcgacgaa tcaatggttg tactaccgga agcgccgccg
     5761 ttacacacgc tgccactggc aaccaaagtc ccggccccct taccgccgct ggaaggatac
     5821 acctttgagg gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggcatc
     5881 accaccagcg tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc
     5941 gatctgctac cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggc
     6001 tgtggcgtgg cgattaacgc accggcggca gttgtgccta ttcgtaccat ccacaatatt
     6061 tcgctgaacc ctaactttgg cggcgaagta atggtgattg gtctgggttg tgaaaagttg
     6121 cagcctgagc gcctgctggt cggcacggat gatgtgcaag ctattccagt agaaagcgcc
     6181 agcattgtca gtttgcagga tgaaaagcat gtcggttttc agtccatggt cgaggatatt
     6241 ttgcaggtcg ccgaacgcca tctacaaaaa ctgaatcaac gtcagcgaga aacctgtccg
     6301 gcttcagaac tggttgtcgg catgcagtgc ggtggcagcg atgccttttc tggcgtaacg
     6361 gcaaacccgg cggttggcta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg
     6421 ttctcagaag tcacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac
     6481 gaagaggtcg gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg
     6541 ggaaaaaccg accgcagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaac
     6601 gtggtagaga aggcactcgg ctccattgct aaatcgggta aaagcgcaat tgttgaagtg
     6661 ctgtcgcccg gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc
     6721 gattttgtct gtggcacgca acaggtggct tcgggtatca ccgtgcaagt gtttacgacc
     6781 ggtcgtggta cgccgtacgg cctgatggcg gtacccgtca ttaaaatggc gacccgcacc
     6841 gagctggcga accgctggtt tgatttaatg gatattaacg cgggcactat cgccaccggc
     6901 gaagaaacca ttgaagaggt gggctggaag ttgttccact ttattctcga catcgccagc
     6961 gggaagaaga aaaccttctc ggatcaatgg gggctgcata accagctggc agtgtttaac
     7021 ccggcaccgg tgacctgatt tcttttggaa cgtccctcgc aaaacatggc cttagtgcca
     7081 tgtttttatt gtttaaagcc cccacgtcca ttaataatgc atttgcatta ccttagttca
     7141 agcttataat ttgagcagaa aacaggctgt aaaaggacag tgaatcatgc ccgctaatgc
     7201 tcgctctcac gctgtactga ccactgaatc aaaggtcacg atacgcggac aaacaactat
     7261 ccccgcacca gtgcgtgagg ccttaaaact gaagccaggc caggacagca ttcattacga
     7321 aattctgcct ggtgggcaag tctttatgtg ccgactggga gatgaacagg aggatcatac
     7381 tatgaatgca tttttgcgtt ttctggatgc agatatccag aacaacccgc aaaaaactcg
     7441 tccattcaac attcaacaag gaaagaaact tgtcgctggc atggacgtca acattgatga
     7501 tgagattggc gacgacgaat aatggatttt ccacaaaggg ttaatggttg ggcgctatat
     7561 gctcatccct gttttcagga aacctacgac gctttagttg ccgaagtcga gacattaaag
     7621 ggaaaagatc ctgaaaatta tcagagaaaa gccgccacaa agttattggc ggtagtccat
     7681 aaagtgattg aggagcatat cacggtcaat ccatcatcac cggcattccg tcatggcaag
     7741 tcgttaggct ctgggaaaaa taaagactgg tcacgggtaa aatttggtgc tggtcgttat
     7801 cgtctcttct ttcgttatag tgaaaaagag aaagtcatca ttctgggatg gatgaacgat
     7861 gaaaacactc tgcgcaccta cggtaaaaaa acagatgcct ataccgtatt cagcaaaatg
     7921 ttaaaaagag gacatcctcc tgccgactgg gaaaccctca cccgagaaac agaagaaacc
     7981 cattgatggt gttcacaatg cccgccgaac gtattcgtgt tgaccagcgg gcaccctttc
     8041 tcactccccg accagaatca cttcaacccc agcctttcgc agtccttcca gactatccgc
     8101 aggaatgcct tcatcaacaa tgatcatgtc gatacgttga gtatcaatga tcttatgtaa
     8161 actggaacga ttgaatttac tggaatcggt gaccacgatg atccgttcca caacttcgca
     8221 catccgacgg tttaaacggg cttcatcttc attatgtgtg ctgacgccgc gctccagatc
     8281 gatcgcatct acaccaagaa acagcatatc gaagtggtaa ttttgcagcg attgctcagc
     8341 ctgatcgccg taaaaagatt gcgactgacg gcgcaaatgc ccgccggtca tcagcagctc
     8401 aacgccttcc gcttccagca acgcattagc cacgttcata ccgttggtca tcgcaattac
     8461 gtcagtgtgc ttacgcatca gacgagcaat ctcaaaagtg gtagtcccgg aatcgaggat
     8521 cacccgatga ccaggctgaa tcaactcaac ggcagctttc gcaacgctgc gtttcatcgc
     8581 ggtgttcagc gcgcttttat cttccactga tggctcgact gacggcgtcg tgctatcgca
     8641 gatcaacgcg ccaccatagg cacgcacagc gatcccctgc ttttccagaa acgccagatc
     8701 attgcggatc gtcacagtag atacgccata caatgccgac agatcgttaa cctgcacact
     8761 cccttgctgt cgcagacgct gaatgatctg ttctcgtcgc tcgctggtgc cagtcactcg
     8821 cttctcacct gaagcgtcgg tattactcat agtaagtcct ttcgtaaaac tttcgtttca
     8881 tttcgttttg cctattaacg cctttctatt aagcaaatgc aagcccacct tgcccattga
     8941 cgcaagctac tctcgtttca gtgactttca ttatgtttct tttgtgaatc agatcagaaa
     9001 actattatct ttcgttttat ttttatctca ccatgacgca gtatcaactg aaacaaaacg
     9061 aaagattaat atcgcagtaa tctgaactgg agaggaaagt gaaacatctg acagaaatgg
     9121 tgagacagca caaagcgggc aaaacaaatg gaatttatgc cgtttgttcc gcacatccgc
     9181 tggtgctgga ggctgcaatc cgctacgcca gtgcaaacca aacgccgtta ctgattgaag
     9241 caacctccaa tcaggtagac cagttcggcg gttataccgg aatgacgccc gccgattttc
     9301 gcggctttgt ttgtcagctc gccgactcgt tgaatttccc gcaggatgcg ttgattctgg
     9361 gtggtgacca tctggggcca aaccgctggc aaaacctgcc ggccgctcag gcaatggcca
     9421 atgccgatga tttgattaaa agctacgttg cggcaggatt caaaaaaatc caccttgatt
     9481 gcagcatgtc ctgtcatgac gatccgattc ccttaactga tgacatcgtg gctgaacgcg
     9541 ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcttga acactttggc gaagccgatc
     9601 tggagtatgt cattggtacc gaagtgccgg tacctggcgg cgcgcatgaa accttaagcg
     9661 agctggcggt caccacgccg gatgccgccc gcgccacgct ggaagcccat cgtcacgcct
     9721 ttgaaaagca aggtttgaat gccatctggc cacgcatcat tgccctggtg gttcaacccg
     9781 gcgtcgaatt cgatcacacc aacgttattg attatcagcc cgccaaagcg agcgccttaa
     9841 gccagatggt cgaaaactac gaaacgctga ttttcgaagc gcactctacc gattatcaaa
     9901 cgccgcaatc gctgcgccag ctggtgattg accactttgc cattctgaaa gttggcccag
     9961 cgctgacctt cgccctgcgt gaagctctgt tctctctggc ggcgattgaa gaagaactgg
    10021 tgccagcgaa agcctgttct ggtctgcgtc aggtgctgga agacgtgatg ctcgaccgcc
    10081 cggaatactg gcaaagccac taccacggtg acggcaacgc gcgtcgtctg gcgcgtggtt
    10141 atagctactc ggatcgcgtg cgctattact ggccggacag ccagattgat gacgctttcg
    10201 ctcatctggt tcgtaatctg gcggattcac caattccgct accgctcatc agccagtatc
    10261 tgccgctgca atacgtgaaa gttcgctccg gcgagctgca accgacgcca cgggaactca
    10321 ttatcaacca tattcaggac atcctggcgc agtaccacac agcctgtgaa ggccaataag
    10381 caaaacaaag aggaacacgc tatgccaaat attgttttaa gccggattga tgaacgcttg
    10441 attcacggtc aggtcggcgt tcagtgggtc ggatttgcgg gggcaaatct ggtgctggta
    10501 gccaacgatg aggttgccga agatccggta caacaaaacc tgatggaaat ggtactggca
    10561 gaagggatcg ccgtacgttt ctggacgctg caaaaagtta tcgacaacat tcatcgcgcc
    10621 gccgatcgac agaaaatcct gctggtttgt aaaacacccg ccgatttcct gacgctggtg
    10681 aaaggtggcg ttccggtgaa tcgcattaac gttggcaata tgcactacgc caatggcaaa
    10741 caacaaatcg ccaaaacggt ttctgtggat gcgggcgata tcgcagcatt taacgacctg
    10801 aaagccgctg gggtggaatg cttcgttcag ggcgtcccga cagagcctgc tgtagacctc
    10861 tttaaattac tttgagggat tcatcatgga aatcagcctg ttgcaggcat ttgcgttggg
    10921 cattatcgcc tttatcgctg gcctggatat gtttaacggc ctaacacata tgcaccgccc
    10981 ggtggtcctc ggcccgttgg tcgggctggt acttggcgat ctgcataccg gaattttaac
    11041 cggcggtacg ctggaactgg tgtggatggg gctggccccg ctggcgggcg cacagccgcc
    11101 taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc attactactg gcgtgaaacc
    11161 cgatgtcgcg gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg ggattacctt
    11221 cctgttctcg gtgatgtctg gcgtgatgtc tcgctgcgac cggatggcgg agaacgccga
    11281 tactcgcggc attgaacgcg tgaactatct ggcactgctg gcactcggca ctttctattt
    11341 tctctgcgct ttcctgccta tctacttcgg cgcggaacat gcaaaaacca tcattgatgt
    11401 cctgccgcaa cgattaatcg acggcctcgg cgtcgcgggc ggcatcatgc cagcaatcgg
    11461 ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac atcccctact tcatcctggg
    11521 ctttgttgcc gcagcctggc tcaagttacc ggtgctggct attgctgccg ctgcgctggc
    11581 gatggcgctg atcgacctgc tgcgtaaatc tcctgaaccg actcaacctg cggcacagaa
    11641 agaggaattt gaagatggca tctaatcaaa ccaccctgcc gaacgtcagt gaaaacgaag
    11701 aaacattgct gactggcgtc aatgaaaacg tgtatgaaga tcagagcatt ggcgcggagc
    11761 tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat gctgttacaa gcgtctttta
    11821 actacgaacg tatgcaggct tctggctggt tgtacggtct gctgcctgct ctgaaaaaga
    11881 tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt ttcttcaata
    11941 cccatccgtt tctggtgaca tttgttatcg gcattatcct tgcgatggag cgttctaagc
    12001 aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctc ggcgggattg
    12061 gcgatgcaat gttctggcta acgctactgc cgatttgtgg cgggattgga gccagtctgg
    12121 cattgcaagg ctccattctt ggcgcagtcg tctttattct gctgttcaac gtggtacacc
    12181 tggggctgcg ttttggtctg gcgcattatg cttaccgcat gggcgtggcg gcgattccac
    12241 tcattaaagc gaataccaaa aaagtcggcc atgctgcgtc tatcgttggg atgacggtaa
    12301 tcggtgcgct ggtggcaacc tatgttcgtt taagcaccac gctggaaatc acagcgggcg
    12361 acgcagtggt taagttacag gctgatgtta tcgacaaact gatgcctgcc ttcttaccgt
    12421 tggtctacac cctgaccatg ttttggttgg tacgccgcgg ctggagtccg ctgcgtctga
    12481 ttgcagttac cgtggttctc ggcatcgtcg gtaaattctg ccatttcctt taaatacaat
    12541 gaggtattag atgttaagta ttattttgac agggcatggc gggtttgcca gcggcatgga
    12601 aaaagcaatg aagcaaattc tcggtgagca atcgcagttt atcgccatcg attttccgga
    12661 aacctcaagc accgcgctgc ttacatcgca gcttgaagag gccatcgccc aactggattg
    12721 tgaagacggc attgtttttc tgacggattt gctgggcggc acgccgtttc gcgtagcttc
    12781 aacgctggcg ctgcaaaaac cgggctgtga agtgatcacc ggcaccaatt tgcaactgct
    12841 actggagatg gtgctggagc gcgaagggtt aagcggtgaa gaattccgtg tacaggcgct
    12901 ggagtgtgga catcgtgggc tgaccagtct ggtggacgag ttaggccgct gccatgaaga
    12961 atgtccggtc gaggaaggga tatgacacac gttctgcgcg ccagaaggct gctgactgaa
    13021 gagggatggc tcgatgacca tcagttgcgt attgctgacg gtgtcatcgc agcaatcgaa
    13081 ccgattccag tgggcgtgac tgaacgcgat gcggaactgc tctgccccgc ttacatcgac
    13141 acccatgtac acggtggtgc gggcgttgat gtaatggatg acgcgccgga tgtactcgac
    13201 aagctggcaa tgcacaaggc acgcgaaggt gtcggcagtt ggttgccgac taccgtaacc
    13261 gcgccgctta acaccattca tgcggcgctg aaacgtattg ctcaacgttg ccaacgcggc
    13321 ggacctggtg cgcaagtgct ggggagttat ctcgaaggac cgtacttcac gccgcagaat
    13381 aaaggcgcgc atccgccgga attgtttcgc gagcttgaaa ttgccgagct ggatcaattg
    13441 attgccgttt ctcagcacac cttacgcgtg gtagcgctgg caccggaaaa agagggggca
    13501 ttgcaggcca tccgccatct taaacagcaa aatgtacgag tgatgctggg gcatagcgcg
    13561 gcgacctggc aacaaactcg cgccgcgttt gatgctggtg ccgacggcct ggtgcattgc
    13621 tataacggga tgacaggttt acatcaccgc gaaccgggaa tggttggcgc gggattaacg
    13681 gacaagcgcg cctggctgga actgatagcc gatggtcatc atgtgcatcc ggcggcgatg
    13741 tcgctgtgtt gttgctgtgc aaaagagaga atcgtgctga tcaccgacgc gatgcaggca
    13801 gccgggatgc cggatggtcg ctatacgtta tgtggcgaag aagtgcagat gcacggtggc
    13861 gttgtccgta ccgcgtccgg tgggctggcg ggcagtacgc tgtctgttga tgcggcagtg
    13921 cgcaacatgg tcgagttgac gggcgtaacg cctgcggaag ccattcatat ggcatcgctg
    13981 catccggcgc gaatgctggg tgttgatggt gttctgggat cgcttaaacc gggcaaacgc
    14041 gccagcatcg ttgcgctgga tagcgggctg catgtgcaac aaatctggat tcagagtcaa
    14101 ttagcttcgt tttgatattt tgctccttta ttgggccttc acttcccccg taaggccttt
    14161 ctttttcttt cgttttgatc tgtgcagcgg tgtcggatgc gacgctaacg cgtcttatgc
    14221 gacctacggt acgccgcatc cagcaatcgg tgaacatagc gacgtcatta cgcaattctt
    14281 taatctcccc ttgtcttttc tttcaatgat cacagatttc gttttatttc ctttctctcc
    14341 attgaacttt cggtttcttt tctatagatt ttaatcaacg aaagacatca ccaagtgaaa
    14401 tgaaacgaaa ggcaagtgaa agcgacaacg cccgacgtca agttcatcag actaaggatt
    14461 gagttatgcc agaaaattac acccctgctg ccgccgcaac cggtacatgg actgaagaag
    14521 agatccgcca tcagcctcgc gcatggatcc gttcactcac caacatcgac gcgctacgtt
    14581 ctgcgctcaa taacttcctt gaaccgttac tgcgcaaaga gaatctgcgg atcatcctga
    14641 ccggagccgg aacctcggca tttatcggtg acatcatcgc gccgtggctc gccagccata
    14701 ccggtaaaaa cttcagcgcc gtaccgacca ccgatctggt caccaatccg atggactacc
    14761 tgaacccagc tcatccgctg ctgttgatct ccttcggtcg atccggcaac agcccggaaa
    14821 gcgtcgctgc cgtggaactg gcaaatcaat ttgtaccaga atgctatcac ctgccgatca
    14881 cctgcaacga agcgggcgct ctttaccaaa acgcgatcaa cagcgataac gcgtttgccc
    14941 tgctgatgcc cgcagaaacg cacgatcgcg gctttgcgat gaccagcagc attaccacca
    15001 tgatggccag ctgcctcgcg gttttcgcac ctgagacgat caacagccaa accttccgcg
    15061 acgtggcgga tcgttgccag gcgatcctga cctcactggg cgatttcagc gaaggtgtgt
    15121 ttggttacgc accgtggaaa cggatcgttt atctcggcag cggtggctta cagggcgcag
    15181 cacgcgagtc ggcgctgaaa gtgctggaac tgactgcggg taaactggcg gccttctatg
    15241 attccccgac cggattccgt catggcccga aatcactggt cgataacgaa acgctggtgg
    15301 tggtatttgt ctcaagccac ccttacaccc gtcagtatga tcttgatctg ctggctgaac
    15361 tccgccgtga caaccaggca atgcgcgtaa tcgccatcgc cgcggaaagc accgacatcg
    15421 tcgctgccgg tccacatatt atcctgccgc cgtcacgtca ctttatcgac gttgagcagg
    15481 cattttgctt cctgatgtac gcccagacgt ttgcactgat gcagtcgctg cacatgggca
    15541 atacgccgga taccccatca gccagcggca ccgttaaccg cgtggtgcaa ggcgtaatca
    15601 ttcatccgtg gcaggcataa gaggatcgca ttatgagcat tatctccact aaatatctgt
    15661 tacaggacgc ccaggccaat ggctacgcgg tgcctgcttt taacattcat aacgccgaga
    15721 cgatccaggc gatcctcgaa gtgtgcagtg aaatgcgatc gccggtgatc ctcgccggaa
    15781 cgccggggac ctttaaacat atcgcgctgg aagagatcta cgccctgtgc agcgcctatt
    15841 ccacaaccta caacatgcca ctggcgctgc atctcgacca ccacgaatcg ctggatgata
    15901 ttcgccgtaa agtccacgca ggtgtgcgca gtgcaatgat cgacggcagc cacttcccgt
    15961 ttgccgagaa cgtgaagctg gtgaaatcgg ttgttgactt ctgccactca caggattgca
    16021 gcgtggaagc agaactgggc cgcctgggcg gtgttgaaga tgacatgagc gttgacgccg
    16081 aaagtgcatt cctgaccgat ccacaagaag ctaaacgctt tgtcgaactg actggcgtcg
    16141 acagcctggc ggtagcgatt ggtacggcgc acggcttata cagcaaaacg ccgaagattg
    16201 atttccagcg gctggcggaa attcgtgaag tggtggatgt tcctctggtg ctacatggtg
    16261 ccagcgatgt tccggatgaa tttgtccgtc gcactattga acttggcgtc acaaaagtga
    16321 acgttgccac agaattaaaa atagccttcg ctggcgcggt taaagcctgg tttgcggaaa
    16381 atccgcaggg taatgatcct cgttattata tgcgcgtcgg aatggatgcg atgaaagaag
    16441 ttgtcagaaa taaaattaat gtctgtggtt cagcgaatcg aatttcagca taatcattag
    16501 ttttttatga tttatcccaa tgtacttccc gattaatatc ggggagtgcc ttaatggaaa
    16561 aggagatgac taaaccttaa taaatacatc agtacaatat cgcaacaata atatattaaa
    16621 aaaaattata ttattcaact ttatggtgag gattacacaa tgagcagtcc aaatattctc
    16681 ttaacccgta ttgataaccg tctggttcac ggtcaggttg gcgtgacctg gacatccacc
    16741 atcggtgcga atctgctggt agtcgtggat gatgttgtcg ctaacgatga tattcaacag
    16801 aaattaatgg gtattaccgc agaaacctac ggctttggca ttcgtttctt tactatcgaa
    16861 aaaaccatta acgtcatcgg caaagctgca ccgcatcaga agatcttcct gatttgccgt
    16921 acgccacaaa cggtacgtaa attggtagaa ggtggcattg acctgaaaga tatcaacgtc
    16981 ggcaatatgc atttctcgga agggaaaaag caaatcagca gtaaagttta tgtcgatgac
    17041 caggatctcg cggacttacg ttttattaaa caacgtggcg tgaatgtttt cattcaggac
    17101 gtccctggcg atcaaaaaga acaaatccct gactaaatct aaaatcgcct taatattggt
    17161 ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg attatccctg gcggcgttag
    17221 ttttcgttct ggggattgat ttttggctgg aagccttatt tttattccgc ccgataatcg
    17281 tttgtaccct aactggcgct attctcggtg atattcagac tggcttaatt accggtggtc
    17341 tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg tgttcagccg cccaacccga
    17401 ttatggcggg tctgatgacc accgtcattg catggtctac gggcgttgat gccaaaacag
    17461 caattggtct tggcctgccg tttagtttgt taatgcagta cgtcattctg ttcttctatt
    17521 ccgctttctc attatttatg accaaagccg ataaatgcgc gaaagaggcg gatacggcag
    17581 cgttttcccg gcttaactgg acaacgatgc tcatcgtcgc ttcagcgtat gcggtgattg
    17641 ctttcctctg tacttacctg gcacaagggg cgatgcaggc gctggtgaaa gcgatgcccg
    17701 cctggttgac ccacggcttt gaagtggctg gcggtattct gcctgccgtt ggttttggct
    17761 tgctgctgcg cgtaatgttc aaagcgcaat atatccctta cctgatcgcc ggtttcctgt
    17821 ttgtttgcta catccaggtc agcaacctgt tgccggttgc cgtactgggc gcaggctttg
    17881 cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc gcaaccgcag cccgttgcca
    17941 gtaaaaatga agaagaggac tacagcaatg ggatctgaaa tcagtaaaaa agatatcacc
    18001 cgtctgggct ttcgttcgtc gctgctgcaa gcgagcttta actacgaaag gatgcaggcg
    18061 ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga tttataagga cgacaaaccg
    18121 ggcttaagcg cggcgatgaa agataacctc gaatttatta atacccaccc gaatctggtc
    18181 ggattcctga tggggttatt aatttcgatg gaagaaaaag gagaaaaccg cgacaccatt
    18241 aaaggcctca aagtggcact gtttggccca atcgccggga ttggcgatgc gattttctgg
    18301 tttactttgt tgccgattat ggcgggaatt tgctcatcat ttgccagcca gggaaacctg
    18361 ctggggccga ttctattttt cgccgtttac ctgcttatct ttttcctgcg cgtcggctgg
    18421 acccacgtcg gttattcagt cggcgtgaag gcgatcgata aagtgcgaga gaactcgcag
    18481 atgattgctc gctcggcaac catcctcggg atcacggtaa tcggcgggct gatcgcttcg
    18541 tatgtgcata ttaacgtggt gacatcgttt gccatcgaca gtacccacag cgtcgcgctg
    18601 caacaggatt tcttcgataa agtcttcccg aacattttac cgatggccta caccctgctg
    18661 atgtattact tcctgcgggt gaaaaaagcg catccggtgc tgttaatcgg cgtgactttt
    18721 gtgctctcta ttgtttgttc cgcattcggc attttgtaaa tggaacgagg cactgcttct
    18781 ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa cccttcagca agttgaaaac
    18841 tatacggcgt taagtgaacg agccagcgaa tatttattgg ctgtgatccg tagcaaaccg
    18901 gatgccgtga tttgcctggc gaccggagcc acgccattac tgacgtatca ttatctggta
    18961 gaaaaaatcc accagcagca ggttgatatc agccagctca ccttcgtgaa gctcgacgaa
    19021 tgggtggatc tgccattaac gatgcctggc acctgcgaaa ctttcctaca acagcatatc
    19081 gtgcagctgc tggggctgcg tgaagaccag ctcatcagct ttcgctccga agagataaat
    19141 gagacagagt gcgaacgggt aacgaacctg attgcgcgca aaggcggtct ggatttatgc
    19201 gttctaggat tggggaaaaa cggtcatctt gggctgaacg aaccgggaga aagcctacaa
    19261 ccggcctgcc atatcagtca acttgatgcc agaacacagc aacatgagat gttaaaaacc
    19321 acgggtcgcc ccgtgactcg tgggatcacc ttaggcctga aggatattct caatgcccgc
    19381 gaggttttgt tattggtgac tggcgaagga aagcaggatg cgacagaacg ttttctcacg
    19441 gctaaagtct ccaccgctat cccggcttcg tttttatggc tgcacagtaa ctttatttgt
    19501 ttaattaata cctaaccaga tgaaaatttg ctcaaaattt ttaaattaac ttattcaa
//
LOCUS       DAZKGS010000076        19313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKGS010000076 DAZKGS010000000
VERSION     DAZKGS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..529
                     /gene="ftnA"
                     /locus_tag="ACHOQY_002757"
     CDS             32..529
                     /gene="ftnA"
                     /locus_tag="ACHOQY_002757"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9678823.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(567..806)
                     /gene="yecH"
                     /locus_tag="ACHOQY_002758"
     CDS             complement(567..806)
                     /gene="yecH"
                     /locus_tag="ACHOQY_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9678824.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            997..2208
                     /gene="tyrP"
                     /locus_tag="ACHOQY_002759"
     CDS             997..2208
                     /gene="tyrP"
                     /locus_tag="ACHOQY_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9678825.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2270..2935)
                     /gene="yecA"
                     /locus_tag="ACHOQY_002760"
     CDS             complement(2270..2935)
                     /gene="yecA"
                     /locus_tag="ACHOQY_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9678826.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3131..3217)
                     /locus_tag="ACHOQY_002761"
     tRNA            complement(3131..3217)
                     /locus_tag="ACHOQY_002761"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3181..3183),aa:Leu,seq:taa)
     gene            complement(3230..3303)
                     /locus_tag="ACHOQY_002762"
     tRNA            complement(3230..3303)
                     /locus_tag="ACHOQY_002762"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3269..3271),aa:Cys,seq:gca)
     gene            complement(3358..3433)
                     /locus_tag="ACHOQY_002763"
     tRNA            complement(3358..3433)
                     /locus_tag="ACHOQY_002763"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3398..3400),aa:Gly,seq:gcc)
     gene            complement(3585..4133)
                     /gene="pgsA"
                     /locus_tag="ACHOQY_002764"
     CDS             complement(3585..4133)
                     /gene="pgsA"
                     /locus_tag="ACHOQY_002764"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9678827.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4190..6022)
                     /gene="uvrC"
                     /locus_tag="ACHOQY_002765"
     CDS             complement(4190..6022)
                     /gene="uvrC"
                     /locus_tag="ACHOQY_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9678828.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6019..6675)
                     /gene="uvrY"
                     /locus_tag="ACHOQY_002766"
     CDS             complement(6019..6675)
                     /gene="uvrY"
                     /locus_tag="ACHOQY_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9678829.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6817..6933
                     /locus_tag="ACHOQY_002767"
     CDS             6817..6933
                     /locus_tag="ACHOQY_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9678830.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            6971..7147
                     /gene="yecU"
                     /locus_tag="ACHOQY_002768"
     CDS             6971..7147
                     /gene="yecU"
                     /locus_tag="ACHOQY_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9678831.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            7134..7358
                     /gene="yecF"
                     /locus_tag="ACHOQY_002769"
     CDS             7134..7358
                     /gene="yecF"
                     /locus_tag="ACHOQY_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9678832.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7426..8148)
                     /gene="sdiA"
                     /locus_tag="ACHOQY_002770"
     CDS             complement(7426..8148)
                     /gene="sdiA"
                     /locus_tag="ACHOQY_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9678833.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8378..9130)
                     /gene="tcyN"
                     /locus_tag="ACHOQY_002771"
     CDS             complement(8378..9130)
                     /gene="tcyN"
                     /locus_tag="ACHOQY_002771"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9678834.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9127..9795)
                     /gene="tcyL"
                     /locus_tag="ACHOQY_002772"
     CDS             complement(9127..9795)
                     /gene="tcyL"
                     /locus_tag="ACHOQY_002772"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9678835.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9810..10796)
                     /gene="dcyD"
                     /locus_tag="ACHOQY_002773"
     CDS             complement(9810..10796)
                     /gene="dcyD"
                     /locus_tag="ACHOQY_002773"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9678836.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10901..11701)
                     /gene="tcyJ"
                     /locus_tag="ACHOQY_002774"
     CDS             complement(10901..11701)
                     /gene="tcyJ"
                     /locus_tag="ACHOQY_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9678837.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11789..12340)
                     /gene="fliZ"
                     /locus_tag="ACHOQY_002775"
     CDS             complement(11789..12340)
                     /gene="fliZ"
                     /locus_tag="ACHOQY_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9678838.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12386..13105)
                     /gene="fliA"
                     /locus_tag="ACHOQY_002776"
     CDS             complement(12386..13105)
                     /gene="fliA"
                     /locus_tag="ACHOQY_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9678839.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13270..14733)
                     /locus_tag="ACHOQY_002777"
     CDS             complement(13270..14733)
                     /locus_tag="ACHOQY_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFP9678840.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLIQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            14988..16400
                     /gene="fliD"
                     /locus_tag="ACHOQY_002778"
     CDS             14988..16400
                     /gene="fliD"
                     /locus_tag="ACHOQY_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9678841.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            16415..16825
                     /gene="fliS"
                     /locus_tag="ACHOQY_002779"
     CDS             16415..16825
                     /gene="fliS"
                     /locus_tag="ACHOQY_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9678842.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            16825..17190
                     /gene="fliT"
                     /locus_tag="ACHOQY_002780"
     CDS             16825..17190
                     /gene="fliT"
                     /locus_tag="ACHOQY_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9678843.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17268..18755
                     /gene="amyA"
                     /locus_tag="ACHOQY_002781"
     CDS             17268..18755
                     /gene="amyA"
                     /locus_tag="ACHOQY_002781"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9678844.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18789..19202)
                     /gene="yedD"
                     /locus_tag="ACHOQY_002782"
     CDS             complement(18789..19202)
                     /gene="yedD"
                     /locus_tag="ACHOQY_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9678845.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         4896 a         4875 c         4571 g         4971 t
ORIGIN      
        1 gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact
       61 taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg
      121 gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga
      181 gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat
      241 taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac
      301 ctataaacac gaacaattaa tcacgcagaa aattaacgaa ctggctcatg ctgcaatgac
      361 caatcaggac tacccaacat ttaatttcct gcaatggtat gtttctgagc agcatgaaga
      421 agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gcggcgaagg
      481 tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat gctctcaggc
      541 ggcaggtttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa
      601 aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc
      661 gcagtcatcc cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta
      721 attgctgctt ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac
      781 acttcatgac cgtgaataga gtccatcgtc cctcctcaaa aaaagcctag cgtagcgatt
      841 gccgcttatg aagactttgc gccagcgcag gactgaatgc tttttattgt acatttatat
      901 ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag
      961 atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt
     1021 gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg
     1081 gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc gttgatgtgc
     1141 tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac cggtcttggc
     1201 acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg
     1261 ttcttaatgt atgctctgac tgcggcatac atcagcggtg ccggtgaatt gttggcctcc
     1321 agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact
     1381 tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt taaccgtttt
     1441 ctgttcagcg ccaaaattat ttttctagtg gtgatgctgg tattgctgct accgcatatt
     1501 cacaaagtga atcttttaac cctgccgttg caacaggggc tggctctgtc agcaatcccg
     1561 gtgattttta cctcatttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat
     1621 ggcaacgttc gtaagctacg ctgggtgttt ataaccggta gtgcgatccc cctggtggca
     1681 tatattttct ggcaggtggc aacgcttggc agcattgatt caacaacctt tatgggattg
     1741 ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct
     1801 ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac gtcatttctc
     1861 ggcgttgcgt taggcttatt tgattatctg gctgatttat ttcagcgttc aaataccgtt
     1921 ggtggacggt tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt tgcactgttt
     1981 tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca
     2041 ttgattatcc cttcgctgtt gacctggcaa agcagaaagc acaatcctca ggcgggttac
     2101 cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt
     2161 ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc
     2221 aaattcctta ttgggtgcca gaattaacgc tgacacccaa tttgacctct taatgcaggc
     2281 agcactgctt aaatttctta ccactaccgc acgggcaagg atcgttacgc cccggtttct
     2341 cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtacgcat
     2401 gtagatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca
     2461 ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg
     2521 gttttaacga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa
     2581 agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg
     2641 gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag
     2701 ccagattcat aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc
     2761 caccccacac ggcaaccagc cactgttccg gttcaatctc ttgcggagaa ctcaacaccg
     2821 ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg ttgtatttgg
     2881 tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct
     2941 tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata
     3001 ctttttgagt ggctttgctg gtgattaaaa attagggagg gtgtaacgac aagttgcagg
     3061 cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attgctttga ttttattctt
     3121 atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt
     3181 ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt
     3241 tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc
     3301 gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg
     3361 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
     3421 gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca
     3481 atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat
     3541 ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa
     3601 tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca
     3661 aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag cagccaggcc
     3721 aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc cacgctactg
     3781 cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc
     3841 atcgttgccg ccggtagggt cacccaccag ctgtgataat gctcggttac cagcaccatg
     3901 gcgatagcca cgagaacttt atctgctaca gggtcaagga aagcaccaaa ccgggtactc
     3961 tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa
     4021 atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat
     4081 gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg
     4141 ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca
     4201 acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct
     4261 cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac
     4321 gttttggccc gacgccttca atggtttcca gggagctggt atttttgacc ttcgcccgtt
     4381 ttttacggtg cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc tggataacat
     4441 gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca aagaacagtg
     4501 tttccagccc agccttacga tctactcctt tggcaacgcc aagtagcagt ggatgatttt
     4561 tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc
     4621 cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc
     4681 gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat
     4741 agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg
     4801 tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt
     4861 tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc
     4921 tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc
     4981 gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca
     5041 gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc
     5101 tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc
     5161 caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata
     5221 cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg
     5281 tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac
     5341 gtgcagcttc ttcaaactcc agattctggc tggcagtttc catacgacta atgagttgcg
     5401 taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt
     5461 attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct atctggtatt
     5521 gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa
     5581 tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat
     5641 attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg gtatcgccac
     5701 tcaggaagat aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat
     5761 agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtatga gtaaccgtta
     5821 catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctac
     5881 ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac
     5941 caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt aaaaacgctt
     6001 ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg
     6061 aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata
     6121 gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt
     6181 ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg
     6241 gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga tgtcagaagc
     6301 aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct gcggagccgc
     6361 gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc
     6421 tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga ttttacgcgt
     6481 cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt
     6541 tgcccgacac cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc
     6601 ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag
     6661 tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca
     6721 ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt
     6781 tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc
     6841 ttaccggatc gtttcacagt aagccctgcg acgaaatgtg acaaaaatta cctttattca
     6901 gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca
     6961 aaaaacattc atgataaaaa tatttattgg tcattatatt aacgcattta atagcaccgt
     7021 tgacgatacg ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc
     7081 cgccttaaca ttattcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta
     7141 cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga
     7201 aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt
     7261 taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca
     7321 aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgatagcgtg
     7381 cctttcagcc ggtttttgca tctggcacgc aggacagaaa agagatcaaa ttaagccagt
     7441 agcggccgcg taacaggcaa cctgggtctt attcggtgca ttgatttttt tctgcatgtt
     7501 tttctgatgg aaattgaccg tattctcaga gattgacaaa atcatcgcta tctctgctga
     7561 tgtcttccct tccgccgtcc acctcagaat ttctttttcg cgcttgctga aattcatctc
     7621 tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt cgcgcaccag
     7681 taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgcg cgctgcaacg
     7741 ggaaaaggac aaaaagccca gcgcccggtt tggcagcatt aaatactgag tgacaccgcg
     7801 gcgtaaacca tgtgcgcgcg cggcttccca taacggctgt gcttcgttga ataagtcatc
     7861 attccacatt agatggccct gactaaagtt ttcagggttc agcaccggat caattgcgag
     7921 aaagtttttt gcctgataat aactaaccca cgcctcaggg taattggtgt agaaagccac
     7981 tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat cgtactccag
     8041 ctgctgagcc tgaagctcta tttcatggta gacctcttct gcggcctcca tcctctgaaa
     8101 acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag taaaccgcaa
     8161 cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata aataagattt
     8221 atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcac attctcttat
     8281 gttattaatg agttatgctg atttgttaag cagttttatc acgcttgaaa tagcgtcccg
     8341 ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga aacttctcga
     8401 ggaactggcg ggtgcgaggc tgctggggat cggcaaataa cgcttttgct gccccctgct
     8461 cgactatccg cccctggtcc ataaaaatcg cccggtccgc aacatcccgg gcaaagctca
     8521 tttcgtgcgt cacaatcacc atcgtgcgct tttcctgcgc cagctgacgg atggtgttca
     8581 ggacttcgcc caccagctct ggatccagcg ccgacgttgg ctcgtcaaac aaaatcacct
     8641 caggacgcat tgccagcgca cgtgcaatcg caacacgttg ctgttgaccg ccagacaaac
     8701 gacgtggata gctggtttct ttaccggcca gcccaacttt cgccaacagc tcgcgagcgc
     8761 gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca ataatgtttt
     8821 ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg acgtgctgac
     8881 gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca atagtgatat
     8941 cgccaacggt gatcgttccc gcttccggtt gttccagcag atttatgctg cgtagcaacg
     9001 tggttttgcc ggaaccactc ggaccgataa ttgccaccac ttcgccaggc tttacctcga
     9061 ggtcgatacc gtgcagcacc gtctgaccgt gaaatttttt caccaagttc ttaacttcaa
     9121 tggcactcat tttggttctc tctcctggcg attaagttga ttctcaaaat ggttctgcaa
     9181 cgtcgataat actgtcgcca tgatccagta gatgagcgaa gccgccaggt acatggtgaa
     9241 cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg gcacctggat
     9301 tgtcgcggcc agcgacgtat cctttaccag gctgataaag ctgttcgaca gcggcggcaa
     9361 cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcata gtctgccatg gcgtcatacc
     9421 aatactggca gctgcttccc actgaccttt atcaatagaa gaaatggcgg cgcgcagtgt
     9481 ttcggcggca taggcggcag tgttcagcga cagaccaatc attgctgacg gaatgggatc
     9541 taattcgata ccaaactgcg gcaggccgta atagatcata aacagttgtg cgatgagcgg
     9601 tgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc agattggcga
     9661 caggcgcatc agcgcgagaa taaacccgag cagtaagcca aaaaacatgc cgccgatact
     9721 gagttgcagc gtatacccag cccctttcag caggaacggc aaggaatcaa taaccagttg
     9781 tatactttct tgcatgagcg ttcccggagc taaacgtggg gatgataggc gaacagcgca
     9841 ggcgcaccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg tttctgactg
     9901 ataccgtcaa tcagccccgc catcgctttt ccggtataca caggatcaag cagaatgcct
     9961 tccagccgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac gccgtagcca
    10021 ggtgcaaaat agtcatccca gagtataatt tccgctgatg cggtcagctc cagttctttc
    10081 gcaatcgcct gttgtaggtt aaccactttc ggcaactgat cggcaacgga acgcgacacg
    10141 gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc cagtccggcg
    10201 tgagttccgg cactgcccga tgcgactacc accgacgaaa tattaaccgc cccttcacac
    10261 tgttgcgcga tttccagcgc actctccaca taacctagcg cgccaagagc attagaaccg
    10321 ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc cagctcttcc
    10381 agttgggcat tgggatcggt cagtgcttcg cacatttcaa tctgggtatt gaacagatcc
    10441 agcaacaagc gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat aggattttcc
    10501 agcagcgcca cacagtgcag accgagtttc gccgcaactg cggcagtctg ccgcacatgg
    10561 ttagactgga tcgccccggc agtaatcagc gtatcggcac cttcgcgcag agcatctgct
    10621 gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggggt gacgtcatcc
    10681 cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata ttcgagcggt
    10741 gttggcgcgc cgataaactc caaccgtgga aaacgggtta aattatgcag tggcataaca
    10801 gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc gtaaataaaa
    10861 aaggcgctgg tgaaagcgcc cttttttgtc attatgctga ttatttggtc acatcagcac
    10921 caaaccattt ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc gcaattgcat
    10981 cgttcactgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca gactcctgac
    11041 gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc agatccagcg
    11101 ccgccagacg gtcaacgagg atcgcatcga tacggcctac gcgcagatcc tgatatttgg
    11161 tcgggtcatc atcataggta cgcacatcga cgccctgaac attctgccgc agccactctt
    11221 catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca gccgttttaa
    11281 tggtgccttc gttacctttt ttcaccagcg cctgaatacc agaaatggtg tacggggtag
    11341 agaaatcgta ttttttcttg cgctcatcag aaatggtgac ctgattaatc accacatcaa
    11401 tgcgtttaga gtccagcgac gccagcatac cgtcccattt ggtcggtttt agtgacgcct
    11461 caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg gttaatttgc
    11521 cgtcatcccc ctgaaaactg aacggcggat aagtgccttc cagccctacc agcagcgtgc
    11581 cgcgctcttt aactttatta agcagacctt catctgcaaa acttttaacg ctcatgcccg
    11641 caaccagcgc tacggccatc acactcatca atgcctgacg tcccagatgt gctaatttca
    11701 tatttacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt gtaaacacaa
    11761 aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc aggctggagg
    11821 atttctgcac aagtccggta cacgtttgac gctgatagtg ttgatacttc cgtaatgcga
    11881 tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca tcaaggaaac
    11941 cgtcctgcag cggatcgagg gaaatatttt gctcgtgtag atgattcccc aaacggcgca
    12001 aacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca aaaagaaatt
    12061 gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa tcaccgcaaa
    12121 atcggcacaa tgccgcccat gatttttgtt ccgtttgcca gccattttca tcaagcagcg
    12181 cgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaacg agggtaatgc
    12241 gatcgagtaa tttacggcaa tgcgcgcaat gagtctggct gtgtttaaag tctttaagat
    12301 agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc tggtagtcaa
    12361 agttaaagtg cggcatttac tgacgttata acttacccag tttagtgcgt aaccgtttaa
    12421 tagcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc gcgccaatct
    12481 ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg cgctccggca
    12541 acgtttcgat ggcttccatc acccgctgac gcagattact gtccagcagt tgttgtagcg
    12601 ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc
    12661 actcatcgta ggagaagagc tggctgttat tggtgtcgag caacatttgg cgataatcgg
    12721 caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg ccaagttcct
    12781 gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca
    12841 cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc actgcgtaag
    12901 ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac ggcattaagt aacccaatgc
    12961 cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca
    13021 atgcttcgtg acgcaccagc gggacataac gctgccatag cgagtgttta tccattacac
    13081 cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag ttatcggcat
    13141 gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg aggggttatt
    13201 tgggggttac cggtaaattg cgggcagaaa aaaccccgcc ggtggcgggg gttgagcgat
    13261 aagtgtaaat taacgcagca gagacagtac gttctgcgtg gtctggttag cctgcgccag
    13321 aacagaggta cccgcttgtt gcaggatctg cgcacgagac atgttagaca cttcggtcgc
    13381 gtagtcagca tcttcgatac ggctacgggc agaagacagg ttgtttacgg tgttgccaag
    13441 gttggtgata gcagagtcga aacggttttg tactgcaccg aggtcagaac gcagattgtc
    13501 aactttagcc aatgctttgt cgatagtttc gagcgggttg gtggtagatt gcaacgattt
    13561 tgctgcatct tcgtttacca gaatcgggct accaccttct gatttgctca gatacatgac
    13621 tttattgtta ccagaagcag tctccgttat cgttttacca tctgcactaa catcgtaagt
    13681 tgcaccgtta acaactaacg tgcttcctgt tttcttagct gcattgagat caagatcgga
    13741 aagtgtggca gccttgttat caactttggt tgtcgtataa aggccagcgc tatttttgta
    13801 aactgtctca ctcgtaatca aagaagcttt tttgtcagca tctgtcacag taaatgtcac
    13861 tgacttacca tcaatttctg cagaatatac accattccca ttgccatccg ctgcagttgt
    13921 tgttgtgaat tcaactccat taaacgtgaa tttatcattc tgtgctttat tcttagccgc
    13981 tgctgcgagt gctgtagcat caatccctgt accagctatg tttgtgtcag atttagttgt
    14041 cagtgaacca tctgctgcac taacaaaaat atcattccct gtagtatctt taacagctcc
    14101 gctatctacg ttaacagtat aagcatcacc gccaacatca tagttatcag tacctgtcgc
    14161 tttaaattta gaaatcaggt cactgccagt tgctttctgc gcgccatcga tattaaaacc
    14221 gtccaggccg agagttttcg catcaatttt tgccagattg atagtgatgg tttcaccatc
    14281 attagcacca acctgaattt tcatttcatt attttcagca aggactttca cgccgttaaa
    14341 ctgagtttgc tcagatacac ggtcaatttc ttccagacgt tgagtaattt cagcctggat
    14401 agaagaaaga tcgctgtcag agttagtacc gttagttgcc tgaacagtca gttcacgtac
    14461 acgctgcagg ttgttgttaa tttcattcag cgcaccttca gtggtctgcg caacagaaat
    14521 accatcattc gcgttacggg aagcctgaat cagaccttta atatttgctg taaaacggtt
    14581 agcaatcgcc tgacctgctg cgtcatcttt agcactgtta atacgcaggc cagaagagag
    14641 acgttcaatg gcggagctca gagaagactg agatttgttc aggttattct gggtcaacag
    14701 cgacaggctg tttgtattaa tgacttgtgc catgatcttt tccttatcaa ttacaacttg
    14761 atgttattgg gctgttgccc acggtttctc accgtacccc ttgtatcggc acctgaattt
    14821 cgaactttag aaaattttag tttctctcct gttttttctt aacggctgaa atatccgtaa
    14881 tatacaactc tactctgcgc atcatcttcg tgaatcaact attaaacttt gcgcacaaat
    14941 tgccgataac ccgcttatct actgtttgca atcaaaagga attaggtatg gcttcattta
    15001 catctcttgg cgttggttcg aatctgccgc tggatacatt gctaaacaac ctgaccatcg
    15061 ctgaaaaaaa acgcttaaac ccaattactc agcaacagag tgacaatacc gcacgtctga
    15121 cagcctatgg cacattaaaa agtgcattag aaaaattcca gactgcaaat actgcgttaa
    15181 ataaagccga tctgttcaaa agcactaccg ttaccagtag caatgaagac cttaaagttt
    15241 ccaccgaagc aggtgctgcc cccggtatct ataccatcag cgtaacccaa ctggcgcagg
    15301 cgcaatcttt acgaactgat tccccgacaa tcattgcatc aacaaaagat gcactgggtg
    15361 atgagtcatc agatacacgc acaataaaaa ttacccaaga cggtcgtaaa gagccgctgg
    15421 aaatcaagct caataaagat cagacgtcat tggatgagat cagcaaagcc attaacgatg
    15481 ccgatagtgg aatttccgct agtatcgtta aagtcaaaga tggtaactat caattggtat
    15541 tgaccgccag tgaaggtctg gccaataaaa tgaccatatc tgttgagggt gacagtaaat
    15601 taaatgatct gctggcttat gacagcaaga ccaacaccgg gaatatgaag gaactggtta
    15661 acgcgcaaaa tgcacagcta aacgttaacg gtatcgatat tgaacgttca agtaacaaaa
    15721 ttaccgatgc tcctcaggga gtgactctcg atttgaccaa aaaagtcact gacgtccggg
    15781 tcacggtgac aaaaagcaac gataaagcaa ccgaagcaat taaaggctgg gtggattcat
    15841 ataactccct cattgacacc tttaacaccc tgacaaaata taaagaagta gacccaggtg
    15901 ctgaagcaca agataaaaat aatggtgcac ttctcggaga cagcgttgta cgtacaattc
    15961 aaagcggcat ccgcgcacaa tttgcaaacg gtgccagcga cagtgccttc aaaacattaa
    16021 acgagatcgg gattaagcag gacggcacga cgggtaagct gaaaattgat gatgataaac
    16081 tcaaaaaagt cctgaatgaa aatacagcat ccgtacgtga attgctggta ggcgatggta
    16141 aagaaacagg aattaccacc aaaattgcca ccgaggtaaa aggttacctt gccgatgatg
    16201 gcattattga tagcgcacag gacagtatta acgccacact gaaaaaactg accaaacagt
    16261 atctatccgt cagtgccagc attgatgaca ccgtggcgcg ttacactgca cagtttacgc
    16321 aattagatac catgatgagc aaactgaata acaccagtac ctatctcagc caacaattta
    16381 ctgcaatgag taattcctga tcacggaggt cagcatgtat accgcgagcg gctcaaaagc
    16441 ctatgcacaa attggcgtcg aaagcgccgt aatgagcgcc agccagcagc agctggtcac
    16501 catgctattt gatggagtgc tgagcgcact ggttagagca cgcctgttta tgcaggacaa
    16561 caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact
    16621 gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttga ttgctcttta
    16681 tagctatatg gtcaggcgct tgctgcaagc caatttacgc aacgatgtct ccgcagtcga
    16741 agaagtggaa gcattaatgc gcaatattgc cgatgcctgg aaagagtcgt tactctcccc
    16801 ttctttgatt caggacccag tctgatgaac cacgcaccgc atttgtattt cgcctggcaa
    16861 caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cagaagaaca atgggacgaa
    16921 ctcatcgcca gcgagatggc gtatgtgaat gcggtgcagg agattgcaca tctgactgaa
    16981 gagattgacc cgtccaccac gatgcaggag cagctccgcc cgatgctgcg cctgattctc
    17041 gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg
    17101 gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggtgatca gggcggcttt
    17161 gtgctggctc cgcaggataa cctcttttga atctgaacga gtcgatggct cgcgaataat
    17221 ccgattacgg ctacgcttct aatgttcccc ttgaatggag tcgaagaatg cgtaatccca
    17281 cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg
    17341 ccgagcgcgc cgacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct
    17401 ataaaggcgc atcgggcggg tattcggtcg gctacgactc ctatgattta tttgatttag
    17461 gcgagtttga tcagaaaggc agcatcccta ctaaatatgg cgataaagca caactgctgg
    17521 ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gttggacgtg gtagtcaacc
    17581 acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc
    17641 gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac accttccccg
    17701 cccgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc
    17761 atatcgaaaa ccctgatgaa gatggcatat ttaaaattgt taacgactac accggcgaag
    17821 gctggaacga tcaggttgat gatgaattag gtaatttcga ttatctgatg ggcgagaata
    17881 tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg
    17941 aacaaacgca atgcgacggt tttcgtcttg atgcggtcaa acatattccg gcgtggtttt
    18001 ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggcag
    18061 agtactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa
    18121 ccatgctgtt cgatgcgccg ctgcagatga aattccatga agcatcgcgc atggggcgcg
    18181 actacgacat gacgcagatt ttcacgggta cattagtgga agccgatcct ttccacgccg
    18241 tgacgctcgt tgccaatcac gacacccaac cattgcaagc cctcgaagca ccggtcgaac
    18301 cgtggtttaa accgctggcg tatgctctaa ttttgttgcg ggaaaatggc gttccttcgg
    18361 tgttctatcc ggacctctac ggtgcgcatt acgaagatgt cggtggtgac gggcaaacct
    18421 atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt
    18481 tcgcccacgg tgtacagacg ttatttttcg accatccgaa ctgcattgcc tttagccgca
    18541 gtggcactga cgaatatccc ggctgcgtgg tggtcatgtc gaacggggat gatggcgaaa
    18601 aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaatc
    18661 ggcaagagag cgtagtgacc gacgaaaacg gcgaagcgac cttcttttgc aacggcggca
    18721 gcgtcagcgt gtgggttatc gaagaggtga tttaaattca tccccggcgg caagccgggg
    18781 agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct
    18841 gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca
    18901 cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat
    18961 gtcaacttgc ccggcacggc aatcacccgc tgccactgac ggcaatccag ggtatcgccc
    19021 tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg ttgcggccct
    19081 ttggtctgcc agtagcccgc tagccagtcc ggcgcggggg ttttcacgac attgttatag
    19141 ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ttgcaattgc gagttttttc
    19201 atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagccctg atgtcgccag
    19261 cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt att
//
LOCUS       DAZKGS010000077        19245 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKGS010000077 DAZKGS010000000
VERSION     DAZKGS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..142)
                     /locus_tag="ACHOQY_002783"
     CDS             complement(<1..142)
                     /locus_tag="ACHOQY_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678846.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLV"
     gene            complement(181..1167)
                     /gene="yeiR"
                     /locus_tag="ACHOQY_002784"
     CDS             complement(181..1167)
                     /gene="yeiR"
                     /locus_tag="ACHOQY_002784"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9678847.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1285..2751)
                     /gene="yeiQ"
                     /locus_tag="ACHOQY_002785"
                     /pseudo
     CDS             complement(1285..2751)
                     /gene="yeiQ"
                     /locus_tag="ACHOQY_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(2974..3546)
                     /gene="yeiP"
                     /locus_tag="ACHOQY_002786"
     CDS             complement(2974..3546)
                     /gene="yeiP"
                     /locus_tag="ACHOQY_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9678848.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            3701..3955
                     /gene="yeiW"
                     /locus_tag="ACHOQY_002787"
     CDS             3701..3955
                     /gene="yeiW"
                     /locus_tag="ACHOQY_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9678849.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(3952..5133)
                     /gene="setB"
                     /locus_tag="ACHOQY_002788"
     CDS             complement(3952..5133)
                     /gene="setB"
                     /locus_tag="ACHOQY_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9678850.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            5501..6631
                     /gene="fruB"
                     /locus_tag="ACHOQY_002789"
     CDS             5501..6631
                     /gene="fruB"
                     /locus_tag="ACHOQY_002789"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9678851.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            6631..7569
                     /gene="fruK"
                     /locus_tag="ACHOQY_002790"
     CDS             6631..7569
                     /gene="fruK"
                     /locus_tag="ACHOQY_002790"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9678852.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            7586..9277
                     /gene="fruA"
                     /locus_tag="ACHOQY_002791"
     CDS             7586..9277
                     /gene="fruA"
                     /locus_tag="ACHOQY_002791"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9678853.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(9370..9516)
                     /locus_tag="ACHOQY_002792"
     CDS             complement(9370..9516)
                     /locus_tag="ACHOQY_002792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678854.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            9701..10642
                     /gene="psuK"
                     /locus_tag="ACHOQY_002793"
     CDS             9701..10642
                     /gene="psuK"
                     /locus_tag="ACHOQY_002793"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9678855.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            10630..11568
                     /gene="psuG"
                     /locus_tag="ACHOQY_002794"
     CDS             10630..11568
                     /gene="psuG"
                     /locus_tag="ACHOQY_002794"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9678856.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            11662..12912
                     /gene="psuT"
                     /locus_tag="ACHOQY_002795"
     CDS             11662..12912
                     /gene="psuT"
                     /locus_tag="ACHOQY_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9678857.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12980..13678)
                     /gene="yeiL"
                     /locus_tag="ACHOQY_002796"
     CDS             complement(12980..13678)
                     /gene="yeiL"
                     /locus_tag="ACHOQY_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9678858.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            13808..14749
                     /gene="rihB"
                     /locus_tag="ACHOQY_002797"
     CDS             13808..14749
                     /gene="rihB"
                     /locus_tag="ACHOQY_002797"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9678859.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            14849..16099
                     /locus_tag="ACHOQY_002798"
     CDS             14849..16099
                     /locus_tag="ACHOQY_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9678860.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16155..17243)
                     /locus_tag="ACHOQY_002799"
     CDS             complement(16155..17243)
                     /locus_tag="ACHOQY_002799"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9678861.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17247..18104)
                     /gene="nfo"
                     /locus_tag="ACHOQY_002800"
     CDS             complement(17247..18104)
                     /gene="nfo"
                     /locus_tag="ACHOQY_002800"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9678862.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18178..19227)
                     /gene="yeiH"
                     /locus_tag="ACHOQY_002801"
     CDS             complement(18178..19227)
                     /gene="yeiH"
                     /locus_tag="ACHOQY_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9678863.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT         4740 a         4688 c         5086 g         4731 t
ORIGIN      
        1 cgaccagttt ctgattaaag aaataaaaaa tatccgcatc aatcggcaac cagaatccat
       61 gattaacggg aatataccag gaaagaaaca gcgcgaggcc gacaatattc aacaacacta
      121 tttgcggcaa atttttaatc atattttctc taattatctt actgaaaaac gcaggcaacc
      181 ttacgcggta gtcgctaaac gaagcttcaa cagcgcgctc tgcaaagcat tccagtcagc
      241 ttcgctgctg gaaatcagct caatacggct gtccggtggc gcaacgtttt gcgtttcaat
      301 gtgcaggtca tcgccctgac ggttgattcg taccagccct tcgggaatac gcagcacgcc
      361 tttgacgcgt tccaccggtg caagtcgcgc ccattccaga atgccaatgg tgtcgaatac
      421 cgtatcagcg tcgaatatcc agccgcaggc ctgatatcct tgcccgctgt tcagactgcg
      481 acgccagcgt tgatgctctg gcaggcttaa cgctgctaac ccttttttca cgacatgctg
      541 atgagaatgc gcggcgctgg cgggcaactc ggctaaattg cgacgcggca aatccagaag
      601 atgaccgtca actttcccat gctcactgtg aattaattgt cgatcgccac cattttgctg
      661 ccaccaacgc tgtagcgctt gctcactttc gggcgtcgca cggtcggatt tattggcgac
      721 aatgatgtct gcggcagcca gctggtcacg gaagttttca ttgctggcgc ttttttcgtc
      781 cagcagcaag cgcggatcga gaatgcacaa ggtggcgcgc agatctatcc acggttcata
      841 gactggtgcg gttaacagat cgaggatctg tttcggatgg cccagcccgg tcggctctat
      901 caacaagcgg tctggttttc cctgacgcag taaggtattc aaccctacct gcatgggtaa
      961 accattaacg cagcacatgc agccgccggg gatctctttc agcaatgcgc cgctatcggc
     1021 gagcaaagca ccatcaattc cgacttcccc aaattcatta accagtaccg cccatttttc
     1081 gttgggatct ttatgggcta acagatgaag aatcgacgtg gttttcccgc tgccgagaaa
     1141 accggtgatg aggttggtcc tggtcacaac ggctccgcaa tactctggta agttaactga
     1201 gcgttaatcc tggctaaaaa aagcagaaaa gagaagggga gagcggacga aggtgagctt
     1261 catccgcttt aaccgcagaa atcgttaaat tttgagggtg tttaatagcg cctgatgtgc
     1321 gccgaattgt acgatttgtt gccaggcttg ttcgatggcc tgcacaaaat gcgggttatc
     1381 tggcagatca tcaccgaaaa cttcacgcag ggaaagcagg gcggttacgc gttgttcact
     1441 gctgctgccc gcaacaagtt cgcgaatttt atcgctaagc ggatcgcgaa catcaatggc
     1501 atttccggca tcatcaacgc cgctgacgta acgcatccag cctgcaacgc ctaatgccag
     1561 caacgaccag tccgtttcgc gcccctgatg tatgcgaata cctgccagca tgcgttgcgg
     1621 taatttctgg ctgccatcca tcgcgatttg ccaggtctta tgtttcagcg ccggattagc
     1681 aaaacgtgca attaacttat ccgcatattg tgttaaatcg acatctttaa tttgcagtgt
     1741 cggcgcttgc tcatccagca ttaatgttct ggcggcatgg cgaaatgcgc gatcctgcat
     1801 gcaatcactg atatgggcga atcctgagag gtaacccaga taagcgagaa aagagtggct
     1861 gccattaagc atccgcagtt tcatctcttc ccatggcagg acatcattca ccatttgtac
     1921 acctgcgact tcctaggcag gacgcccagc gacgaagtta tcttccacca cccactggat
     1981 aaacggttcg cagctaatcg cgcagggatc attcaccccc agatgctggc tgatttccac
     2041 cagtgattcg tcggttgcag ccggaacaat gcggtcgacc atggttcccg gaaaactgac
     2101 gtgctctttt atccacccgg cgagttctgg cgaacgtttt tctgccattc ccagcaccgc
     2161 gtttttcacc acatgaccat tgtcgggaat attgtcgcag gagagcacgg taaacggtgt
     2221 aaggccgcgc tcacggcggc gtttcagtgc ttcgacgaga atacccggtg cggagtgagg
     2281 ttcttcaggg gtttgtagat cgtgaataat ccgcggatta ctggtgtcga gtgcaccggt
     2341 ggccgggtca atacaatagc ctttttcggt aatcgtcagg gaaacaattg ccacctgtgg
     2401 ctcgcaaaat ttctcaataa ttgccgctaa ggaatccagt tttgcattaa ggcattcgtg
     2461 aacggcaccg acaattatca cctgattgcc gtccgcacct ttctccagca cggtatataa
     2521 atggttctgt gcgcggagct ggctcatcag ttgatcaccg ctgaacaagc tgatttcaca
     2581 gatcccccag tcgccgccct gggcattcag cacacgatcg gtcagtaacg cctgatgagc
     2641 gcggtgaaag gcgccaaagc caaaatgaac gatacgtgat tgcaactgct ggcgatcgta
     2701 gcgtggagcg tgtacatgat gcgggagcgt aacggaggca attgtcttca taggagtcac
     2761 gtcttagtaa cgataaatta acaatgacag tgtaaagtta tatgacagca attgaaattg
     2821 gtctggattg ttaatgcgat gaagtgtgag ggcgatcaat caacgagtga aaattgaggg
     2881 cgtaaggtgt tcaggaagcg caaaatagaa gattggtata ataaattaaa aaagggacag
     2941 cctgagctat cccttttctg catgcggctg aagttagtca gcacgcccca tataacggcg
     3001 ttcttcgata tggatacgaa ttttttcgcc cgggcttaag tattctggta cctgaatcac
     3061 cagaccagtg ctcaatgtcg ccggtttgtt acgggcgctg gcagatgccc ctttgatgcc
     3121 tggtgcggtt tcaacgattt ccagatcaac ggtctgcggc agctcaagcg ccagcagttg
     3181 accgtcccag gtcagcacct gcatgtccgg catgccgccc tctggcataa acagcaactc
     3241 ttcttcaatc tgatctttgg tgaaggtata cggggtatag tcttctttat ccataaagac
     3301 atattcgttg ccatcgacat aggagaagtc aacgtaacgg cgggtcagcg tcacggtgtc
     3361 aacgatatca tcacctttga aacgctcttc tactttcagc ccggtacgga catcagaaaa
     3421 acgcattttg tacagcgtag cggcaccgcg ggcagtgggc gactgaatat caatatcctt
     3481 aaccagcagc agtttgccgt tgtaattcag taccatacct tttttaattt cgttcgctct
     3541 tggcatcgaa aaattcctgt agtcgaaaat gtcaaaaata tcgcgacaaa gtactcgcga
     3601 cgggcctttc gggcaagtgg tattcgcact tttgctggtg caaaaaaggt ggtactgtgc
     3661 gcgctcatca atccggtggt taaccttaag agaacaacgt atggaatgcc gtccgggttg
     3721 cggggcgtgt tgcactgccc cttcaatttc cagccctatt cccggtatgc cagatggcaa
     3781 gcccgccaat acgccctgca ttcagcttga tgaacagcaa cgctgtaaaa ttttcacctc
     3841 gccactgcga ccgaaagtct gtgcaggctt acaagccagc gctgaaatgt gcggtaactc
     3901 gcgtcagcag gccatgacct ggcttatcga tctggaaatg ctgaccgcgc cttaaacatc
     3961 tttaatccgc agtaagcaaa acagagtggc gataatcatc accatcgcaa accagaacac
     4021 agcgtgataa ttccagatct cggcgacgat gcccgccact gatcctgcga tgatccagcc
     4081 cacgcgcgaa gtgttggtat agagcgtggt ggctgaaccc gcctgaccgg gcatcagatc
     4141 ctgaaaatag agcatcccga tgccgcccag aatgccaata aaaatagcat ttagcagctg
     4201 caagcccaac agaatgacag gtgaatgcgc catcagcatt cctgcgtaaa aacagacgcc
     4261 acccacggca gcaacgcgca ttaagaaacg cttacccaga cgtttggcga aatatccggc
     4321 aatcaacatc gtcgggattt ccagcccggc ggcggtcccc atcatcacac cggccagttt
     4381 ctcgggaaga tgcagttcgt tgataataaa tagcggcatg ttgatgatgt acaggctgtt
     4441 cgagccccac attaatgtac aaatgacaaa cagcagtagc gtatcgcgac ggttacggcg
     4501 cggcgcttca accgtgccgg tcgccagcgg aagctctttt tgcatcgacg gtaaaaacag
     4561 ccacaccatc acgccgcaaa caataaacgc taccgctgcg ctcagataca ttaccgtaaa
     4621 gctgaaaccc atcgctaacg cataagccag cggtgggcca atgacccatg ccagtgaaac
     4681 ctgagcgcgt aaaaaagagc tgaacatcac cgcctcacgt ccggttttgt cggcatgttc
     4741 acgggcaagg gcaaacattt gcgggttagc ggtcgagcca aagctgctaa gaaagacgcc
     4801 aacgaatagc aaaacaaagt agttgcgatt ccaggcaaaa agggtgcagg ccagcacgcc
     4861 taacaggcag caaaagacaa tcagcgattt gcgatcgccg cgcttatcag agcgcccggc
     4921 gagaaactga cttaccagaa tcccaatgac agcgctgccg gtaaagaaga atcccaccat
     4981 cgccggacgg gcatgtactt catcggtaag aaaaatactg agtgtcgggg tttgcagagc
     5041 gcccgcaata ccggtgagaa aggcaacgat taaaaacgcc gtcgaggtca ggtcaaacga
     5101 tttcgcgctg gagactgcgg gggagttatg catgctggtc gatcactgga agaggaagac
     5161 gcggagttta cgctgcttgt cagaaaataa acagacagcg aaatcaaaag cgccacgaat
     5221 aatcaaaatg ccatttcact gttgctgatt ggctgctgac gctgctgaaa tgaaattgct
     5281 gataacggat tttcccatca gcaattagga aaaatgacaa aaaattgtgc agcacatcaa
     5341 acttttgctc ataactttac ggctttcctt gcgtgctgaa acagaagtat tatgctttct
     5401 tgaaacgttt cagcgcgatc ttgtctttaa ccctaagcca ggttggcgct ttttttctca
     5461 tagaagctga atcgtttcaa ttcagcaaga gaggagaact atgttccagt tatccgtaca
     5521 ggacatccat ccgggcgaaa aggccggaga caaagaagag gcgattcgcc agctcgctgc
     5581 ggcgctggtg caggccggta acgtagcaga aggctacgtc aatggcatgc tggcgcgcga
     5641 acagcaaacc tcaacgttcc tcggcaatgg tattgctatt ccacacggca ctaccgacac
     5701 ccgcgatcag gtgctgaaaa ccggcgttca ggtatttcag ttcacggaag gcgtcacctg
     5761 gggtgacggt caggtagcgt acgtagcgat cggtattgct gccagctcgg atgaacatct
     5821 gggcctgcta cgccagctga cccacgtact gagcgatgat tccgttgctg aacaactgaa
     5881 gtcagcaaca acagcagaag aacttcgcgc attgctgatg ggcgaaaagc agagtgagca
     5941 gctgaagctc gacaacgaaa tgctgacgct ggatatcgcc gccagcgatc tgctgactct
     6001 tcaggcgctg aacgctgcgc gtctgaaaga ggcgagggca gttgacgcca ctttcgtcac
     6061 caaagccatc aatgaacaac cgctgaacct cggacagggt atctggctga gcgatagcgc
     6121 cgaaggcaat ctgcgtagcg cgattgcggt aagccgtgcg gcaaatgctt ttgatgtgga
     6181 cggcgaaacg gcagccatgc tggtgagtgt ggcgatgaat gacgatcagc ccattgcggt
     6241 tcttaagcgt ctcgctgatt tgttgctcga caataaagct gaccgcttgc tgaaagcgga
     6301 tgcggcaacg ttgctggcgc tgctgaccag cgatgatgcg ccgaccgacg acgtgttaag
     6361 cgcggagttt gtggtgcgca atgaacacgg cctgcatgct cgtccaggta ccatgctggt
     6421 caacaccatt aaacaattta acagtgatat taccgtgaca aaccttgatg gcaccggcaa
     6481 accggcaaac ggacgtagtc tgatgaaagt tgtggcactt ggcgttaaga aaggtcatcg
     6541 cctacgcttt accgcccagg gtgcagatgc tgaacaggcg ctgaaagcaa tcggcgacgc
     6601 tatcgctgct ggtcttgggg agggcgcata atgagcagac gtgttgctac tatcaccctt
     6661 aatccggctt atgaccttgt tggtttctgc ccggaaattg aacgcggcga agtgaacctg
     6721 gtgaaaacca ccggtctgca tgcggcgggt aaaggcatca acgtggccaa agtattaaaa
     6781 gacctgggaa ttgatgtcac cgttggcggc ttcctgggta aagacaatca ggatggtttt
     6841 cagcaactgt tcagcgagct gggcattgcc aaccgtttcc aggttgtaca ggggcgcacc
     6901 cgaattaacg ttaagctgac ggaaaaagac ggcgaagtga ccgacttcaa cttctcgggt
     6961 tttgaagtca cccccgccga ctgggaacgc tttgtgactg attctctgag ctggctcggt
     7021 cagttcgata tggtctgtgt cagcggaagc ttaccgtcag gcgtcagccc ggaagcgttc
     7081 accgactgga tgactcgcct gcgtagtcag tgtccttgca ttatctttga tagtagccgt
     7141 gaagcgttag tagcaggttt gaaagcggca ccgtggctgg tgaaacctaa ccgccgcgag
     7201 ttggaaatct gggcaggccg taaactgcct gaaatgaaag atgtgattga agctgcacat
     7261 gcgctacgtg aacaaggtat tgcgcatgtt gttatttcac tgggtgccga aggcgcgctt
     7321 tgggttaatg cctccggcga atggatcgcc aaaccaccgt cagtcgatgt cgtaagcacc
     7381 gttggcgcag gggattctat ggttggtggc ctgatttatg gcttgctgat gcgtgaatcc
     7441 agtgaacaca cactgcgtct ggcgactgct gttgcagccc tggcggtaag tcaaagcaat
     7501 gtgggtatta ccgatcgtcc gcagttggcc gcaatgatgg cgcgcgtcga cttacaacct
     7561 tttaactgac agcaggagag gcataatgaa aacgctgctg attattgacg ctaatctcgg
     7621 tcaggcacgc gcctatatgg cgaagaccct gctgggcgcg gcggcgcgaa aagcaaaact
     7681 ggaaatcatc gacaatccga acgacgcaga aatggcgatt gttctcggtg attccatccc
     7741 gaatgacagc gcgctgaacg gtaaaaatgt ctggctgggt gatatttccc gggcagttgc
     7801 gcaccctgag ctgttcctga gtgaagccaa aggccatgcg aaaccttaca ctgcgccggt
     7861 cgctgcgaca gcaccggttg ccgccagcgg tccgaaacgc gtagttgcgg tgactgcttg
     7921 cccgactggc gtagcacaca cctttatggc ggctgaagcc attgaaaccg aagcgaaaaa
     7981 acgtggctgg tgggtgaaag ttgaaacccg tggttctgtt ggtgcgggta atgcaatcac
     8041 ccccgaagaa gtagccgcag cggatctggt gattgtggcg gcagatatcg aagtggatct
     8101 ggcgaaattt gctggtaaac cgatgtatcg tacctctacc ggtctggcgc tgaagaaaac
     8161 cgcgcaggaa ctggataaag cggttgctga agcaacgccg tatgaaccgg cgggcaaagc
     8221 tcaaacggcg acctctgaag gtaagaaaga gagtgcaggc gcataccgtc acttgctgac
     8281 gggcgtctct tacatgctgc cgatggtcgt tgcaggtggt ctgtgtatcg cgctttcttt
     8341 tgcttttggt atcgaagcgt ttaaagagcc gggtacgttg gcagcggcgc tgatgcagat
     8401 tggtggtggt tcagcctttg cgctgatggt gccggtactg gcaggttaca ttgccttctc
     8461 cattgccgat cgtccgggtc tcacgccggg tctgattggc ggtatgctgg cggtcagcac
     8521 cggttctggc ttcattggcg gtattattgc gggcttcctg gctggttaca ttgcgaagtt
     8581 aatcagtacg caactgaaac tgccacagag tatggaggcg ctgaaaccga tcctgatcat
     8641 tccgctaatt tccagtctgg tggtcggtct ggcgatgatc tacctgatcg gtaaaccagt
     8701 tgctggcatt ctcgaagggt tgactcactg gctgcagacc atggggactg cgaatgcggt
     8761 tctgctgggg gcgatcctcg gtggcatgat gtgtactgac atgggcggtc cggtaaacaa
     8821 agcagcgtac gcattcggtg tgggtctgct gagtactcaa acctatggcc cgatggcggc
     8881 gattatggcg gcaggtatgg tgccaccgct ggcaatgggt ctggcaacaa tggtggcgcg
     8941 tcgcaaattc gacaaagcgc agcaggaagg tggcaaagcc gctctggtat tgggactgtg
     9001 cttcatttcg gaaggtgcaa ttccgtttgc tgctcgtgat ccgatgcgtg tgctgccgtg
     9061 ttgtatcgtt ggtggggcgc tgactggcgc aatctcaatg gcgattggtg cgaaactgat
     9121 ggcaccgcac ggtggtctgt ttgttctgct gatccctggc gctattacgc cggtactggg
     9181 ttacctggta gcaattattg ccggtacgct ggtggcgggt ttggcctatg ccttcctgaa
     9241 acgtccggaa gtggacgcag tagcgaaagc cgcgtaataa aaggtgtgtt acagggcaga
     9301 aatttactgc cctgtatcat tggatgagcg caaagagaat gaccggtttg tgaatgtaat
     9361 tttatgaatt taaatggttt tatctttcag tagaaacctg aaacgttcat caaacactaa
     9421 ggttatttcc tcaagggcag attcttttct gccctttttt agtgttcctg ttctgtgcat
     9481 cccgtcacaa attcctcgct tttgtaattc tggcatttaa tcgcattttg tgagcctgct
     9541 caaatctgcc cgctttgcct ctctggcggc aatttacttt acgtaaaaca tacatactat
     9601 gagcacatgt ttaaatatga acacatgttt aaactgtctt tgcagtatct atattcttgt
     9661 ggattgacag ggttggtaat tttttaccag aggacgtgct atgcgcgaaa aggattatgt
     9721 cgtaattata ggttcggcga atattgatgt cgccggatat tcacatgaat cattaaatta
     9781 tgcggattca aatccaggta aaataaaatt tacacctggt ggagtagggc gaaatattgc
     9841 acaaaacctg gcgttgctgg gtaacaaagc ctggttactg agcgcagtag gtagtgattt
     9901 ttatggtcaa tcgctgctaa cgcaaaccaa tcaatctggc gtttatgtcg ataaatgcct
     9961 gattgtgccg ggagaaaata cgtcgagtta tttatcatta ctcgataata ccggtgaaat
    10021 gctggttgct ataaatgaca tgaatattag caacgctatt acagctgaat atctcgcaca
    10081 gcaccgtgaa tttattcaga gggcaaaggt cattgtcgcg gactgtaata tcagtgaaga
    10141 ggcactggca tggattctgg ataatgctgc caacgtaccc gtatttgtcg atccggtttc
    10201 agcgtggaaa tgtgtcaaag tgcgcgaccg tctaaatcag atccacactc tgaagccaaa
    10261 ccgccttgaa gcggaaacac tgagtgggat tgcgctgtca gggcgtggag atgtggcaaa
    10321 agttgctgcc tggttccatc aacatggcct gaaccgactg gtattgagca tgggcggcga
    10381 tggcgtttat tacagtgata tcagcggtga aagtggctgg tctgccccga tcaaaaccaa
    10441 tgttattaat gttaccggag cgggcgatgc catgatggcg ggacttgctt cgtgttgggt
    10501 agacggaatg ccgtttgccg aatctgttcg tttcgcacag ggatgttcgt caatggcgct
    10561 ctcctgtgaa tacaccaata accccgattt atcgattgcc aacgttatat cgttagtgga
    10621 gaacgcagaa tgtctgaatt aaaaatttcc cctgaattat tacaaatttc cccggaagtg
    10681 caggacgctt taaaaaacaa aaaaccggtt gtggcgctgg aatcgaccat tatttctcac
    10741 gggatgccgt tcccacaaaa tgcccagacc gcaattgaag tagaagaaac tattcgtaaa
    10801 cagggcgcag tacctgccac tatcgccatt attggcggcg tgatgaaagt gggtttaagc
    10861 aaagaagaaa ttgaattact gggtcgtgaa gggcataacg tgaccaaagt tagtcgtcgc
    10921 gatttacctt ttgttgttgc cgccggaaaa aatggcgcaa ccactgtggc ttcaacgatg
    10981 attattgcgg cgcttgccgg aattaaagta tttgccaccg ggggaattgg tggtgtgcat
    11041 cgcggggcgg aacatacctt cgatatttct gccgatttgc aagaactggc aaatactaat
    11101 gtcaccgttg tttgtgccgg ggcgaaatct attctcgatt taggattaac aacagaatat
    11161 ttagaaacct tcggtgtgcc gttaattggc tatcagacaa aagcgctgcc tgcgtttttc
    11221 tgccgtacca gcccatttga agtcagtatt cgccttgata gtgccacgga aatcgctcgt
    11281 gcaatggcag tgaaatggca aaccggtttg aacggcggcc tggtagtggc gaatccgatc
    11341 ccggaacagt ttgcgatgcc ggaggaatct atcaatgcag ccatagatca agccgtcgcc
    11401 gaagccgaag agcagggcgt tattggtaaa gaaagtacac cgttcctgct ggcgcgcgtt
    11461 gctgagctga ccggcggtga cagcctgaaa tccaatatcc agctggtgtt caacaacgcc
    11521 attctggcga gtgaaattgc caaagaatac cagcgtctcg cgggttaatt tctgacgggc
    11581 agaaagccgt cgcccgtact catcaagcat accaaaacct ggcgctgacg ccgggtttac
    11641 tggcatgaag ggaaaatcat tatggatata atgagaagtg ttgtggggat ggtggtgtta
    11701 ctggcaatag catttctgtt gtcagtgaat aaaaagagca tcagtttgcg cacggttgga
    11761 gccgcactgc tgctgcaaat cgctattggt ggcatcatgc tctacttccc accgggaaag
    11821 tgggcagtag aacaggcggc attaggcgtt cataaagtga tgtcttacag tgatgccggt
    11881 agcgccttca tttttggttc gctggttggg ccgaaaatgg atgtcctgtt tgacggtgcg
    11941 ggttttatct tcgcctttcg cgtacttccg gcgattattt tcgttactgc gctcatcagt
    12001 ctgctgtact acattggcgt gatggggctg ctgattcgca tccttggcag cattttccag
    12061 aaagctctta acatcagcaa aatcgaatct tttgtcgcag tcacaactat tttcctcggg
    12121 caaaatgaga tcccggcgat cgttaaaccg tttatcgatc gcatgaatcg caacgagttg
    12181 tttaccgcaa tttgtagcgg gatggcgtcc attgctggtt cgatgatgat tggttatgcc
    12241 ggaatgggcg taccaattga ctacctgtta gcggcatcgc tgatggcgat cccaggtggt
    12301 attttgtttg cacgtattct tagcccggcc accgagcctt cacaggtcac atttgaaaat
    12361 ctgtcgttca gcgaaacgcc gccaaaaagc tttatcgaag cggcggcgag cggtgcgatg
    12421 accgggctga aaatcgccgc tggtgtagcg acggtggtaa tggcgtttgt cgcaattatt
    12481 gcgctgatca acggcattat cggcggaatt ggtggctggt ttggtttcgc caatgcctct
    12541 ctggaaagta tttttggcta tgtgctggca ccgctggcgt ggatcatggg tgtggactgg
    12601 agtgatgcca atcttgcggg tagcctgatt gggcagaaac tggcgattaa cgaattcgtc
    12661 gcttacctga gtttctcccc atacctgcaa acgggcggca cgctggaagt gaaaaccatt
    12721 gcgattatct cctttgcgct ttgtggtttt gctaactttg gttctatcgg tgttgtcgtt
    12781 ggcgcatttt cggctatttc gccaaaacgc gcgccggaaa tcgcccagct tggtttacgg
    12841 gcgctggcag cagcaacgct ttccaacctg atgagtgcga ccattgccgg gttctttatt
    12901 ggtttagctt gagtttcatt gccggatgta cgcgcttatt aagtgttgca tccggcaatc
    12961 gcgctattgc aaataatttt tactgcatca tcccggagaa tttattctcc gggtccatct
    13021 ccagcgccag tcctgacaac tgctttctgt ttttaattag ataccctttc ttgcttttaa
    13081 ttaataaacc gtcgtgaatg aattgcgcga gaacatataa aagatgtcga taagaaacgc
    13141 ctaaatactc tgccgcttgc gtatgctttt cgtgataaag atcaccttcc tgcgagagta
    13201 atataaatgc tgccaggcga ttcactaacg gaaatgattg attctgagtt aaagaaacaa
    13261 tattacgata atttttatga cttaaggtga cgcagagttt tcgtaaaaat agcgtgtcgt
    13321 ttaataaccg cgggcggtaa tgtttcatag ggagcgcaag gcaccaacac tcttcaatag
    13381 cctgcaccgc acgcggttca tggtctttgt cgattaactc aatctcgcca ataaaacagg
    13441 gggcggcaaa gaaatcgatc agcgacacgc gaccattagc tagcgtggcg taaagcctgg
    13501 cgcggcctcg cgtcaggtaa aacagccagg acggctgttg cccttcctgc acaatgtagt
    13561 cacgcgctaa aaaatgaaac agccgcgtat cggctgaaac atccgttaaa aagcaatcct
    13621 taaacgcgga ttcactcatc aattgctgct taagatcatt attgtggatt tctttcacga
    13681 tgtttatccg ctatgagatt tctcatattt agcatacgta cgcggttggt aagattcaat
    13741 catcggcccg gtcgaatttt ctttgctgta gaaaaaataa cgcaactgga aacagaggaa
    13801 ataaacaatg gaaaagagaa aaattattct ggattgtgat ccgggtcatg atgatgctat
    13861 tgcaatgatg atggcagcga aacatccggc aatagattta ttaggcatca ctattgtagc
    13921 gggtaatcag acgcttgata aaacattaat taatggcctg aatgtttgcc agaaactgga
    13981 gattaatgtt ccggtttacg cggggatgcc gcaacccatt atgcgtcaac aaatcgttgc
    14041 cgataatatt cacggtgaaa ccggactgga tggcccggta ttcgagccgc taacccgcca
    14101 ggcagaaagt acccacgcgg tgaaatacat cattgatacc ctgatggcaa gcgatggcga
    14161 tatcactctg gtgccggttg gtccgctttc aaatatcgcg gtggcaatgc gtatgcaacc
    14221 cgcgatcctg cccaaaatcc gtgaaattgt gctaatgggc ggcgcttacg gtacaggcaa
    14281 cttcacgcca tctgccgagt tcaacatctt tgctgacccg gaagcagccc gcgtggtgtt
    14341 cacctctggc gttccattag tgatgatggg cctcgatctc accaaccaga ccgtttgcac
    14401 cccggacgtg attgctcgga tggaaagggc aggcggccct gccggagagc tgttcagcga
    14461 catcatgaac ttcactctca aaacgcagtt cgaaaactac ggcctggctg gcggcccggt
    14521 gcacgacgcc acctgcatcg gttatctgat taaccctgat ggcattaaaa cccaggagat
    14581 gtacgtcgaa gtggacgtca acagtggccc ttgctatggg cgcaccgtct gcgacgagct
    14641 gggcgttctt ggcaagcccg ccaataccaa agtcggcatc actattgata ccgactggtt
    14701 ctggggctta gtcgaagagt gcgtgcgcgg ctacatcaaa acccattaag cgttgataac
    14761 aagccgggcc gatagcgcaa gatgcaggcc cggctatttc tgatgggcag aaagccgtcg
    14821 cccaatatga catgaagaga taatcactat ggatgtcatg agaagtgttc tgggaatggt
    14881 ggtattgctg gcaattgcat ttttgctgtc agtaaacaag aagaagatca gcctgcgtac
    14941 cgttggcgcg gcgttagtgt tacaggttgt gattggcggc attatgcttt ggttaccgcc
    15001 agggcgttgg gtcgctgaaa aagtcgcttt tggcgtgcat aaagtgatgg cgtacagcga
    15061 cgcgggtagc gcatttatct tcggttcgct ggtcgggccg aaaatggaca cgctgtttga
    15121 tggcgcagga tttatctttg gtttcagagt attaccggca attatcttcg ttacagcact
    15181 ggtgagtatt ctctactaca tcggtgtgat ggggatttta attcgcattc tcggcggtat
    15241 cttccagaaa gcgttaaata tcagcaagat cgagtcattc gtcgcggtca ctaccatttt
    15301 cctcgggcaa aacgaaattc cggcaatcgt caaacccttt atcgatcgtc tgaatcgcaa
    15361 tgaattattt acagcgattt gtagtggcat ggcctcgatt gctggttcga caatgattgg
    15421 ttacgccgcc ctgggcgtac ctgtggaata tttgctggcg gcatcgttaa tggcgatccc
    15481 aggcgggatc ttgtttgccc gcctgttaag cccggctacg gaatcttcgc aggtttcctt
    15541 taataacctc tctttcaccg aaacaccgcc aaaaagcatt attgaagccg ctgcgacagg
    15601 ggcaatgacc gggctgaaaa tcgccgccgg tgtagcgaca gttgttatgg catttgtcgc
    15661 catcattgcg ttaattaatg gtattatcgg cggcgttggc ggctggtttg gttttgcaca
    15721 tgcctcgctg gagtccattt taggttacct gttggcccca ttggcgtggg tgatgggggt
    15781 tgactggagt gatgcaaatc ttgccgggag tttgattggg cagaagctgg cgatcaatga
    15841 atttgtcgct tatctcaatt tctcgccata tctgcaaacg ggtggcactc tggatgctaa
    15901 aaccgtggcg attatttctt tcgcgttgtg cggtttcgct aactttggtt ctatcggggt
    15961 ggtggtgggg gcgttttctg cggttgcgcc acaccgtgcg ccggaaatcg cccaacttgg
    16021 tttacgcgcg ctggcggcgg cgacactttc taacctgatg agtgctacta ttgcaggatt
    16081 ctttattggt ttagcgtagt gcgcaattta tgagccggat aagatgcggt caagcgtcga
    16141 atccggcaac attgttacac cattggcact aatgaaagcg cgttatcggc agacagggta
    16201 gggttgttta agctgccgct ggcgcgcgaa attgccgcgc aggctaccgc aaaacgggcg
    16261 ctgtcgcgga aactgtatcc ttcaagaaag ctatatacca ggcccgccat aaaaccatcg
    16321 tcagcaccaa aactgtctac tgtcgtatgc gctggcgcgg tcagcaaaaa ttgttctcca
    16381 tccttatcgc tgcaaaatac agattcatct ttcagataaa caaaaatgcg tttaacacct
    16441 tgttgatgca gcgaatttac agctctgatg cgatcgttgt cgtctttaat cggttgcccc
    16501 cacaaaattt ccagttcatt ttgtgttggt ttcagagtgt ggatccggct gtaccagttt
    16561 tttactttgt tcgctttaaa ttcggaaacg gtatcgacaa acatcggaat ttcatcagca
    16621 atggtaaaga cccactccag ggcttcaggt gtgagattac aatcagccag caccacacca
    16681 gcatggcgaa tcaagtcccg tgaagtattg agcagttgag gcgttagctg ttgcagaata
    16741 tgtgtgtcat tgattgccag gatagtttct tcctgcttat tagcgatagc gagataagtt
    16801 gcagtgctgt gtccatgcag acgaatgcag tttgaaacgt tcacgcccgc gcggcgcgtt
    16861 tcttccagta atgtctcgcc gtaaaaatcg ttgccaatag ctgatattaa atgcacatcc
    16921 cgaccaagca gagccaggtt atgcgcgata ttacgtccga cgccgcctgc ggaacagtga
    16981 acagaacctg gatgagaggc tgcttgcggg taacggatat ccgccattcc gcgaatatcc
    17041 atattgattg cacccacaac cacacagtaa tcctgctcgg taaggatata tcctttgcct
    17101 ttaatcaaac ccttgcgcat caaatccata atatgagcgg caacacgtga gcggctgatt
    17161 tgcagaatat cggcgatttc attctgttga atcagcgggt tacgccgtaa aattttaaga
    17221 atctgctttt ccctgtcgtt catcgatcag gccaccgctt tttcagtttg ttgcgctttc
    17281 agccaggcga tctcttctgc ccagatatcc gggttgatgg tttcaaggat cagcggaatg
    17341 ccgtcgaaac ggtcgtcctg catgatccag cggaacgcat catgaccgat attgccttca
    17401 ccgaggctat gatggcggtc aacgcggctg ccaaaggtgc ttttcgcatc gttaaggtgc
    17461 atcccgcgca gatacttaaa gccaacaata cgggcaaaat cagcgaacgt tttttcgcat
    17521 tcggctggag tacgcaaatc atacccggca gcgaaagcat ggcaggtatc aatgcagacg
    17581 ccgacacggg atttatcttc cacgccgtcg ataatcgccg cgagatgttc gaatttaaac
    17641 cctaagttac tgccctgacc ggcggtgttt tcaatcaccg ctgttacacc ttgagtttta
    17701 tccagcgcaa tgttgatgga ttcggcaata cgcgcaaggc aatcctcttc tgaaatctgc
    17761 atcagatggc tgccagggtg gaagttgagc aaagaaagcc ccagttgttc gcaacgctgc
    17821 atttcatcta taaaggcatc gcgcgatttt tccagagctt cagtgaccgg atgtccgagg
    17881 ttaatcagat agctgtcgtg gggaagaatt tgcgccgatg tgtagtgata tttttcacag
    17941 gccgctttga attcatcgat ggtttgcgtc gtgagcggtg cggcacgcca ctgacgttgg
    18001 tttttggtga acaaggcaaa cgcggttgcg tcgatttcgg cggcgcgaat tgcggcattt
    18061 gccagaccgc cagcagcact aacgtgcgct ccaatgtatt tcatgcgagg actcctgtta
    18121 aacccgctgg aggaaaacgg taatgatagc gggttaacag gagcaagatg tagtggttta
    18181 tgcgatgacg ctttgaatca catagttaat cgcaccacca ccaacaatca gccaggcaaa
    18241 cagtaccagt gccatcagca gaggtttcgc cccggctttt ttcagcgcgc tgacgtgagt
    18301 ggtcagaccc agcgccgcca tcgccattgc cagcaggaag gtatccagcg ttaccagcat
    18361 gttcaccacg ctctgcggta acaggtggaa cgagttaaag atggcaacta cgatgaacaa
    18421 gatggcaaac cacggaatag tgattttgct tttctcgccg ctgttcgccc cagacagctg
    18481 tttaacacgc gccgccagca ggatgaggaa cggagccagc atcatcacgc gcagcatttt
    18541 ggaaataact gctgcgtttt ccgcatccgg gctgatggca tgacctgccg ccaccacctg
    18601 cgccacttcg tgcacagtag aaccaatgta gataccgaaa gtttccggac taaaccattg
    18661 agacatcagc ggatatatcg ccgggtagag gaaaatagcg acggtcccga agataacaac
    18721 ggttgcaaca gccacggtta ctttactggc ttccgctttc actaccggct cagtcgccag
    18781 taccgcggca gcaccacaga tactgctacc ggcaccgatc aaccagctgg tgtgcttatc
    18841 cagaccaaac actttctgcc ccaggaagca agccagcagg aaggtactgg acagcgtcaa
    18901 cacgtcaatg atgatcccac tgataccgac atcggcaatt tgcgagaacg tcagacggaa
    18961 gccataaaga atgataccca gacgtaataa atattgcttg gcaaacagca caccaccgtc
    19021 acagcttttc cagatgtgcg gatagatggt gttgcctaaa accatcccca acaagattgc
    19081 gagggtgagg gcactaaacc cggcacccgc aaccgcggga atggaaccac cccacagggc
    19141 gaccccggtg ataactgcac tcagggctaa ccccggaata aaatgccaca gtgtacgatg
    19201 ttgtttctgt aaggtgatat tcgtcataac cctctccttt aaccg
//
LOCUS       DAZKGS010000078        19132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKGS010000078 DAZKGS010000000
VERSION     DAZKGS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..1007)
                     /locus_tag="ACHOQY_002802"
     CDS             complement(138..1007)
                     /locus_tag="ACHOQY_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9678864.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHOQY_002803"
     CDS             complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHOQY_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9678865.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2467..3033
                     /gene="efp"
                     /locus_tag="ACHOQY_002804"
     CDS             2467..3033
                     /gene="efp"
                     /locus_tag="ACHOQY_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9678866.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHOQY_002805"
     CDS             3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHOQY_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9678867.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHOQY_002806"
     CDS             3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHOQY_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9678868.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3649..3966
                     /gene="sugE"
                     /locus_tag="ACHOQY_002807"
     CDS             3649..3966
                     /gene="sugE"
                     /locus_tag="ACHOQY_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9678869.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHOQY_002808"
     CDS             complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHOQY_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9678870.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4585..5718)
                     /locus_tag="ACHOQY_002809"
     CDS             complement(4585..5718)
                     /locus_tag="ACHOQY_002809"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9678871.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHOQY_002810"
     CDS             complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHOQY_002810"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9678872.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHOQY_002811"
     CDS             complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHOQY_002811"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9678873.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHOQY_002812"
     CDS             complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHOQY_002812"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9678874.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHOQY_002813"
     CDS             complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHOQY_002813"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9678875.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9417..10394
                     /gene="epmA"
                     /locus_tag="ACHOQY_002814"
     CDS             9417..10394
                     /gene="epmA"
                     /locus_tag="ACHOQY_002814"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9678876.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHOQY_002815"
     CDS             10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHOQY_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9678877.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGLSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHOQY_002816"
     CDS             12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHOQY_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP9678878.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            12478..12792
                     /locus_tag="ACHOQY_002817"
     CDS             12478..12792
                     /locus_tag="ACHOQY_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP9678879.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHOQY_002818"
     CDS             complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHOQY_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9678880.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHOQY_002819"
     CDS             complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHOQY_002819"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9678881.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHOQY_002820"
     CDS             complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHOQY_002820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9678882.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            18378..18923
                     /gene="orn"
                     /locus_tag="ACHOQY_002821"
     CDS             18378..18923
                     /gene="orn"
                     /locus_tag="ACHOQY_002821"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9678883.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(18936..>19132)
                     /locus_tag="ACHOQY_002822"
     CDS             complement(18936..>19132)
                     /locus_tag="ACHOQY_002822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678884.1"
                     /translation="LLNFAFVIFAFLRRYGRRIIREIFPSATPQKSFFCVFISSAEII
                     SKTFNLASKSRRRKWIPALT"
BASE COUNT         4419 a         4886 c         5000 g         4827 t
ORIGIN      
        1 caggcattgc aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg
       61 catccggcat gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg
      121 cattagcaaa gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt
      181 aagtgatgca gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct
      241 acggtgcgaa catagacctg acatggcaca cccgcgagtt ttttcagtcg ttggctgaaa
      301 tcaagcattc ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc
      361 accactgcgc cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc
      421 agataatggt cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga
      481 ttgccatttt cctggcagtc aacgtcatat aacggcagga aatctaacag tgaggtaata
      541 cgtaatgcca gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgcgccagc
      601 atttcaatct gcaactcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat
      661 acgaaggtaa gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta
      721 atatcggcat ttttaagctc atcaaccaga atttctggga tgttttctgc cattttctta
      781 atgactttat cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt
      841 tgatgcatac gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag
      901 aacaacgatt ctttattgcg aggttcttta attttcagcg ccagtgcaat gaaattatta
      961 ctcttgcgaa taacaccggt attaacaccc ttgatgctta tggccatgta ggttctccct
     1021 aaccatttct caattaaata attaatttta atttataagc cagataaatg ggcttggtag
     1081 taatagttgt taaaataaca taaatagccg tactcactct atataaacag aaaagaaagg
     1141 ttaattattg gtgttggcta tataaagagt aacaacagca atgtatatga ataattaaag
     1201 gttatgcaac gggcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt
     1261 tgttgctctt gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc
     1321 attcaatttc aatgcacaat gtaggcctga taagcgtagc gcatcaggct aatgtttaac
     1381 ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt
     1441 cgccgccgat ttctcgcgcc agtttcggca ccagataacc cgacaccagt gtcagcaact
     1501 cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt
     1561 tatcgagcac atgcaggtaa tacggcatta cgccggcatc gaacaacgca ttactcaggt
     1621 ttgccagcgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg ttaagcaggg
     1681 tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct acctcattgg
     1741 catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag cgttcaacca
     1801 gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt
     1861 tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca
     1921 tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact
     1981 caagagcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac
     2041 ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gcccggttgt
     2101 ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg tcggtggaga
     2161 atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt
     2221 ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct
     2281 ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa
     2341 gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg
     2401 tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag
     2461 ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac
     2521 ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt
     2581 gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct
     2641 actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac
     2701 ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa
     2761 gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt aactctgtgg
     2821 aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc
     2881 gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac cctgtctact
     2941 ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa agtggatacc
     3001 cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg
     3061 caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc
     3121 agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat caaacatctc
     3181 ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa atcatcagat
     3241 ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca
     3301 ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg
     3361 tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg
     3421 atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa taagcaataa
     3481 cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa ggtaatagtt
     3541 gaaattcccc tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg
     3601 caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt
     3661 atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac
     3721 ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt cagtatggcg
     3781 ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt gtggacgggt
     3841 attggcgcag tcggcgcggc catcaccggc attgtgctgc tcggtgagtc cgctaacccg
     3901 atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact
     3961 cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc
     4021 gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc
     4081 cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca
     4141 ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt
     4201 ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta
     4261 tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact
     4321 ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga
     4381 taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg
     4441 caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat
     4501 gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc
     4561 cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc
     4621 gactctcgct ggattgggat agtttttgtt agccagcatc actataccca gctctttttc
     4681 tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat gtacccatga
     4741 tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg gggtgtgctg ccagtgcaat
     4801 tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca gcatttccca
     4861 gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc
     4921 ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcaggc
     4981 catatcttca atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac
     5041 atgaactgcc ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac
     5101 attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc
     5161 aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc
     5221 atacagacgt tgtgttcctg gcgcccatgc aggctgccag ttttgataga agcgcagcaa
     5281 gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag cagtgtaggt
     5341 tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt
     5401 tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc
     5461 cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt
     5521 tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc
     5581 caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat
     5641 atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca
     5701 gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc
     5761 ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg
     5821 atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc
     5881 atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg
     5941 aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca
     6001 cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata
     6061 attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc
     6121 tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac
     6181 gatggtggca accacagtta ccgcccagag acttttgata attggctctg gtcccatttt
     6241 ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg
     6301 cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa
     6361 gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag
     6421 ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata
     6481 aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt
     6541 cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt
     6601 ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc
     6661 aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct
     6721 ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg
     6781 gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt
     6841 tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac
     6901 cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga
     6961 tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa
     7021 ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt
     7081 taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt
     7141 ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat
     7201 aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca
     7261 cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc
     7321 gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta
     7381 atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg
     7441 tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg
     7501 ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg
     7561 tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg
     7621 cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt
     7681 tccggcgtac ggtagatacc gcaaccttct tccattgcca ggcccatttc gtcgcggatc
     7741 ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca
     7801 gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc
     7861 gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt agaacccaga
     7921 cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg
     7981 cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt
     8041 accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg
     8101 aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca
     8161 tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa
     8221 actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc
     8281 gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct
     8341 tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac
     8401 tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca
     8461 ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc
     8521 ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc
     8581 acgccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc
     8641 tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc
     8701 ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg
     8761 ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt
     8821 gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct
     8881 actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc
     8941 ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt
     9001 gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct
     9061 acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca
     9121 cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc
     9181 tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt
     9241 tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca
     9301 tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc
     9361 tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga
     9421 gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga
     9481 ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt
     9541 gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg
     9601 gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga
     9661 aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg
     9721 aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat cgaccgcact
     9781 atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc
     9841 cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc
     9901 tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agcaatgttg
     9961 ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt ggcgtagagc
    10021 caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac
    10081 tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag
    10141 gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct
    10201 ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga
    10261 atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg
    10321 atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg
    10381 ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg
    10441 ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt
    10501 tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca
    10561 gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca
    10621 cacgataaaa aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg
    10681 atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat
    10741 attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc
    10801 ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat aatagtgtcg gaccacgttt
    10861 tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc
    10921 cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg
    10981 gcgtattgct ggactggagc ctacgcaggt ggttggtctg ctggcagtgg catggatgat
    11041 tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
    11101 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
    11161 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta
    11221 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
    11281 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
    11341 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
    11401 cctcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg gcaatattac
    11461 ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga
    11521 agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
    11581 ttataccggt gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac
    11641 gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat
    11701 cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg
    11761 cggtaccgca tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct
    11821 tccttacctg ttcctcgcgc tggctttccc attttttaaa gcacgtcagg atctcgacag
    11881 accatttgtg attttcaaaa cgcatatgtc ggcaatgatt gcaacagtgg ttgtcgtact
    11941 ggtggtgaca tttgcgaacg tcttcaccat cattcaacct gtggttgaag ccggagactg
    12001 ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat
    12061 ttaccagaac tattgcagca gagtggcaaa aaatccgcag tgggcggtgg aataacccga
    12121 tgccctatcg tctacctgac gatagggcgt aaagacagga aatgttatgg atgacaccag
    12181 ccgggacccg gcaataaccg aagatgaaat cagggcgctg caattttctg ccggggatgt
    12241 agcggaaata gagcagacga ttctctcttt tgtcgatgcc tgccacaccc gtaaagtggc
    12301 gatggtggtg ggtagcacca ttaacaccct caaagatcgc gatggtaaac ggtggggaaa
    12361 cctgccggat atttattgcg cttatcttat ccgatgtctg gtttttcgcg gggaattagt
    12421 gggatacggc gatttgtttc gtatgcgcta tagcgaaatt aagcgaccgg ttaccttatg
    12481 agtgcgcgca tgtttgtctt atgctgcatc tggtttattg tggcgttcct ctggataacc
    12541 atcacttccg cgctggacaa agagtggatg attgatggac gcgggattaa caacgtctgc
    12601 gatgtgttga tgtatctcga agaagacgat acccgtgatg ttggtgtgat aatgacgtta
    12661 ccgctctttt tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg ctggtttatg
    12721 tacgccaccg cgctggctat tttcggctac tggctgtggc agttttttct gcgctatcag
    12781 ttttgtttgt gagccggatt ggttcatccg gcacacaaac ttacaaactt cccgcctgac
    12841 gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg ctttccagac
    12901 gcatctggaa gggcgggaat ggcatatcga taccgtgggc gtggaagcct gccagaatca
    12961 gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggcg taaatacgca
    13021 gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa gacttccggt gccgggttgt
    13081 cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcgctat
    13141 tggcatcggc aggggccggt atcgtcaaca ccacgcgcgt gaccgagtca gagagcgacc
    13201 agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct ttacggtccc
    13261 agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggta agatcgcgga
    13321 tcgttaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcagg ccagagataa
    13381 agttggcgaa aatctcctgc aaaccaaaac cgagaccaac accgagcgcg gcaaccagcc
    13441 actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg ccaatcagca
    13501 tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt aaatccaggt
    13561 gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc gtggtgatga
    13621 taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga ctttctacgc
    13681 cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg aaagcggaat
    13741 ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa attgagcgca
    13801 ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa
    13861 ttgccccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgcg cgttgcgcta
    13921 acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc tggatcagca
    13981 tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt
    14041 caagcctcgc taacagtgcc tgcgccgttg ccagataacc caccgctgac gccagaatgg
    14101 caaccagtgg tgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc
    14161 tgccctcttt gttgaggtac agcgggatcc cggccttttt caggctgagg gtgaccaccg
    14221 ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag aattcacggt
    14281 cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc ccgatgctca
    14341 tcaggtagta gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa
    14401 acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg
    14461 tggccgttac gccatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc
    14521 cgtaacccag cgtcatccac agcaccggca gcggtgacgc gacgagaatc gaccagaaaa
    14581 gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac
    14641 gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg
    14701 cgccaaacag cggcaaaatc gtctctttgc tggtcagcat catcacactg gctttgccca
    14761 actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagtggcc
    14821 aggcgatggt catcgggcgc acgtcagagg tccagaacag atagcgatgc gtcgcttcat
    14881 tcacctcttt cagcgcatcc tcaagttgcc cgttggagac tttcagcttg gtcagttcca
    14941 gcagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgtagct
    15001 gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatgaattt
    15061 gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc acacgcaact
    15121 gcgccatttc ggtatcaagc tgttgtggtt tcggcatttc cggcagccgt gccacctgcg
    15181 cccgcaacgc ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg
    15241 tattcagcgc ctgccgaacc tgtaacgtct ggctggtggc ctggcgctgt tgcgaggcaa
    15301 cgagatccat ccgctgcgcc tgttgattca gtgccgccga tagttcgcgg ttaattttga
    15361 attgcgcgac gatgtctttc ggcaaatcgg cgctgttttc tgccagcaat tcggtacttt
    15421 ccagcgcccg ctccgcctca agctgacgtt ggctgttcaa ctgattacgt aaggcctgca
    15481 aatacgcatc cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt
    15541 cctgacggtt attggcagac agctgcgcca gttccagttc atcaacgagc gctttcaggc
    15601 gtgcggagtc agactgcaac gcgaaatttt gtgcctgatt gagcggggta ttgccagtaa
    15661 gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg
    15721 gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac
    15781 ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag
    15841 acattcccgg cgacacgctg cgcggctcgt cacgcatgtt gtttaattgt gcgcgcagag
    15901 tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg
    15961 aaccttttcg ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct
    16021 gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg
    16081 ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc
    16141 gcacgtcagc gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc
    16201 tggcgtcgtg ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg
    16261 taacaaaggt ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt
    16321 ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg gtagagccga
    16381 gtttaaagcg gcccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg
    16441 caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga
    16501 ccgtctcaat gctgcctaca atcgtcgccc cgaccagaat ctgcgccatc gggccaaatt
    16561 cggtatcgaa caggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag
    16621 cggtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca cgcagaatac
    16681 cgttgcacgg catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg
    16741 taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt
    16801 agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa
    16861 cgccatcggc aggcatgacc aggacattcg gatcggtatc gattgggcgt acttcgtcac
    16921 gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg
    16981 cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc
    17041 atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa
    17101 tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc
    17161 gttcctgatc cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt
    17221 agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc
    17281 tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg
    17341 cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac
    17401 ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga
    17461 ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca
    17521 gccgagcggc ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca
    17581 ggatctcttt ttgcagcccc agcaacgcat tcagcaggct ggatttgccg acgccagact
    17641 gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt agcccatcct
    17701 gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata tccatctgct
    17761 cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acaataatcg
    17821 gctcaatctg caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct
    17881 ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata ggttttacgc
    17941 cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct tccacgatcc
    18001 ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg
    18061 ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt
    18121 ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca
    18181 gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt
    18241 tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtaca gactcctggt
    18301 cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg
    18361 aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga
    18421 tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg
    18481 ccaacctgaa tattctggca gaagggccga ccattgcagt acaccagtct gatgaacagc
    18541 tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg
    18601 tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa tttttaaaac
    18661 agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggtcag gaccgtcgtt
    18721 tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat ctcgatgtca
    18781 gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc
    18841 aggggacgca tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc
    18901 gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg
    18961 cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc acttgagata
    19021 aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc
    19081 ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag ta
//
LOCUS       DAZKGS010000079        18705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKGS010000079 DAZKGS010000000
VERSION     DAZKGS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..892
                     /locus_tag="ACHOQY_002823"
     CDS             <1..892
                     /locus_tag="ACHOQY_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFP9678885.1"
                     /translation="ALPPMPRHANPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYIK
                     EVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSYENDELTYTP
                     GADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERP
                     VKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVKATLL
                     SSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDTADRL
                     NYQKMAQVVDGVINLLYNSK"
     gene            995..1498
                     /locus_tag="ACHOQY_002824"
     CDS             995..1498
                     /locus_tag="ACHOQY_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9678886.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1565..3022)
                     /gene="nuoN"
                     /locus_tag="ACHOQY_002825"
     CDS             complement(1565..3022)
                     /gene="nuoN"
                     /locus_tag="ACHOQY_002825"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9678887.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3029..4558)
                     /gene="nuoM"
                     /locus_tag="ACHOQY_002826"
     CDS             complement(3029..4558)
                     /gene="nuoM"
                     /locus_tag="ACHOQY_002826"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9678888.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4722..6563)
                     /gene="nuoL"
                     /locus_tag="ACHOQY_002827"
     CDS             complement(4722..6563)
                     /gene="nuoL"
                     /locus_tag="ACHOQY_002827"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9678889.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6560..6862)
                     /gene="nuoK"
                     /locus_tag="ACHOQY_002828"
     CDS             complement(6560..6862)
                     /gene="nuoK"
                     /locus_tag="ACHOQY_002828"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9678890.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6859..7413)
                     /gene="nuoJ"
                     /locus_tag="ACHOQY_002829"
     CDS             complement(6859..7413)
                     /gene="nuoJ"
                     /locus_tag="ACHOQY_002829"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9678891.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7425..7967)
                     /gene="nuoI"
                     /locus_tag="ACHOQY_002830"
     CDS             complement(7425..7967)
                     /gene="nuoI"
                     /locus_tag="ACHOQY_002830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9678892.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7982..8959)
                     /gene="nuoH"
                     /locus_tag="ACHOQY_002831"
     CDS             complement(7982..8959)
                     /gene="nuoH"
                     /locus_tag="ACHOQY_002831"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9678893.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8956..11682)
                     /gene="nuoG"
                     /locus_tag="ACHOQY_002832"
     CDS             complement(8956..11682)
                     /gene="nuoG"
                     /locus_tag="ACHOQY_002832"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9678894.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11735..13072)
                     /gene="nuoF"
                     /locus_tag="ACHOQY_002833"
     CDS             complement(11735..13072)
                     /gene="nuoF"
                     /locus_tag="ACHOQY_002833"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9678895.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13069..13569)
                     /gene="nuoE"
                     /locus_tag="ACHOQY_002834"
     CDS             complement(13069..13569)
                     /gene="nuoE"
                     /locus_tag="ACHOQY_002834"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9678896.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13572..15374)
                     /gene="nuoC"
                     /locus_tag="ACHOQY_002835"
     CDS             complement(13572..15374)
                     /gene="nuoC"
                     /locus_tag="ACHOQY_002835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9678897.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15468..16130)
                     /gene="nuoB"
                     /locus_tag="ACHOQY_002836"
     CDS             complement(15468..16130)
                     /gene="nuoB"
                     /locus_tag="ACHOQY_002836"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9678898.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16146..16589)
                     /gene="nuoA"
                     /locus_tag="ACHOQY_002837"
     CDS             complement(16146..16589)
                     /gene="nuoA"
                     /locus_tag="ACHOQY_002837"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9678899.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17220..18158)
                     /gene="lrhA"
                     /locus_tag="ACHOQY_002838"
     CDS             complement(17220..18158)
                     /gene="lrhA"
                     /locus_tag="ACHOQY_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9678900.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4690 a         5175 c         4811 g         4029 t
ORIGIN      
        1 tgcattaccg ccaatgcccc gacacgcaaa tcctgagcaa ttagaaaaaa cagtgcgtta
       61 tcttacacaa actgtgcatc cacgtagtgc cgacaatatt gataatctga ataggtccgc
      121 agagtatata aaagaggtct tcgtcagtag cggtgccaga attacctcgc aggacgtccc
      181 cattacgggt ggcccctaca aaaacattgt tgctgattat ggtcctgccg atggaccgct
      241 gattattatt ggtgcgcact atgactctgc cagcagttat gaaaacgatg aattgaccta
      301 tacacctggc gcggatgata acgccagcgg cgtggcagga ttactcgaac tggcacgttt
      361 gttacatcag caagtaccga aaacaggcgt gcagctggtc gcctatgcgt cggaagaacc
      421 gcccttcttt cgtagcgatg aaatggggag cgcggtacat gcagcttcgc ttgagcgtcc
      481 agtaaaatta atgatagcac tggagatgat tggctattac gactctgcgc ctggtagcca
      541 ggattaccct tacccggcaa tgtcctggct ttatcccgat cggggagact ttattgccgt
      601 ggtcggcaga atgcaggata tcaacgccgt tcgtcaggta aaagcgacat tgttatcatc
      661 ccggtattta tctgtttatt ctatgaatgc cccagggttt attcccggta ttgatttctc
      721 tgatcacctt aattattggc aacacgatat tcccgccgta atgattactg acaccgcttt
      781 ttatcgtaat aaacaatacc acttgcccgg tgataccgca gacagattga attatcagaa
      841 aatggctcag gtggtggatg gtgttataaa tttgttatac aacagtaaat aataaaaatt
      901 tctcgaggcg ggtccagaaa aagcgacatg cacattttat tttccctcgt ctgttttaag
      961 atgcgaaaca tgaaattcaa tggaggaaaa ataaatggga atgattggtt attttgcaga
     1021 aattgactca gaaaagatta accaactgct tgagtctact gaaaaacctt tgatggataa
     1081 tatacatgac acgctttcag gattacgccg actggatatt gataaacgct gggatttttt
     1141 acactttggg ctgacaggca cctctgcttt tgatcccgcg aagaatgatc cgctgagtcg
     1201 tgccgtgctt ggtgaacaca gcctcgaaga tggcatagat ggcttcctgg gattgacctg
     1261 gaatcaggag ttagccgcga caatcgaccg actggaatca ctggatcgaa gtgagctgcg
     1321 taaacagttt tccattaagc gactcaatga aatggagatt tatccgggag tgaccttttc
     1381 agaagagtta gaggggcaac tctttgcttc gatcatgctt gatatggaaa agctgattag
     1441 cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg ttgggtgatg
     1501 gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact tacaaactaa
     1561 cagattacat cagcggcatt gctaaccgca caatgctaat cagcggttgt ggccatacac
     1621 ccagcaccag taccaacagt gcagaaatca gcaccacgat accgcccgcg ctgtactgcc
     1681 agtttgatgg tgcatcgcga cccggttgtt ccggggcgtg aagatacagg ctcaccgcca
     1741 cgcgcaggta gtagtagagg ccgattgccg aaccgacaac cacggcaccc accagccacc
     1801 acaagtgtgc ctggacaccg accgccagca cgtagaactt accgataaag cccagcgtca
     1861 tcgggatacc ggccagagac agcatcatca ccgtcatcac tgccgcgagg atcggacgat
     1921 gccagaacag accgcggtaa gagaacaggg aatcagcatc cgggccacga tacgggctgg
     1981 acatcaggct gaccacgccg aacgcgccga ggctgctgaa cagataaccg gccaggtaaa
     2041 cccctaccgc ttccatcgac atctcaccag tttgcagcgc aatcagcgcc accagcagat
     2101 agccgaggtg agagatagat gagtaaccga gcagacgttt gatgttggtc tggctcagcg
     2161 ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagtact acgcgaatcg
     2221 cttcgctgtc acccaccggt gcgtagagga acagacgcat caccacaccg aagatagcga
     2281 ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg
     2341 gcgtccacag gtggaacggc accagcgaga gtttgaagcc gaggccaaca atcatcagtc
     2401 cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccgaggttt ttacccagcg
     2461 cgacaaacga caggtcgcca gactgtgcat acaccagcgc cataccaaac agcaggaaag
     2521 aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagcgaa cgtttctggc
     2581 ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcaata ccgaggaaca
     2641 aagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca attaacacca
     2701 acaggtagaa ctcatccttg ttgtcgttat agccttcaag ccacgggtag gcgaaagtac
     2761 aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa ccatcaacgc
     2821 gcatcagcgg cgtaacgtcc atagcgcccg cctggccgac aaaccagagc gaaaccagcg
     2881 ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa
     2941 tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc agtgcgatca
     3001 ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg gaattaacaa
     3061 accactgctg gatattgcca atcgcggagc gcgaggtatc cagaatcggc tgcggataga
     3121 agcccagcag taccagcagc accaccagca acaggatcat aaacagctca cgcagcgaca
     3181 tccctggcag ttcctggctg gcaatctggc ttttcgcttt accgaagtaa gcgcgatgca
     3241 acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata acggtaatca
     3301 ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag ttaccggtgc
     3361 caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc catttcatct
     3421 tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca taaagctgac
     3481 cacaaagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg attaccgcgc
     3541 cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc atgtgggaaa
     3601 ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc cacgcaccgt
     3661 agaagatgcc gataacaccc agccacatag caattggcgc gaactctgcc gacgcgttcg
     3721 ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc aagattcccg
     3781 cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc cagccatgca
     3841 gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt aacaggtatt
     3901 ccacaccact ggacattggc gtattcagca gctcttcata gttgaaggtc cagacgccgg
     3961 tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc aggccactcg
     4021 cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg tcagaggctt
     4081 tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc cagaagaaga
     4141 agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg atccacatca
     4201 ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat agtaccgcca
     4261 gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca atggcgagat
     4321 gaatagagat accaaaacgc gggatccacg gcatgtcgaa ttcagactgc cactgcggaa
     4381 ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc gacagcgcca
     4441 gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca aagcgttcgg
     4501 tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt agtaacatgg
     4561 cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat tctcacgaca
     4621 gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc ttatccgacc
     4681 tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag taccatcaac
     4741 agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg cagatagccg
     4801 ttctcactta acagcagacc tttacctgca aagcgggaaa ggacagccgg gatgttcatc
     4861 attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg cttgacgaac
     4921 actttgtcat acagccagtc aaatccccag gcgttgtacc accaggtgcc cagcagacgg
     4981 cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca cagccaggct
     5041 gccagcagaa tgccgaccac cgcgaccacg ccagaggtaa tttccagggt caacatgctg
     5101 ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac aatcagtgcg
     5161 ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt tacccctttc
     5221 acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat acggaaggtg
     5281 tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat atgaccattc
     5341 gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac cagcggtagt
     5401 gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat agatttacgc
     5461 agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac ggaaccggat
     5521 gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat cgccgcatcc
     5581 catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt agagtaagcg
     5641 agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc
     5701 gccccgacaa tacccaccag atgcagaact tccggcgtca ccaggaacag gccgtgggta
     5761 cgggcgatca ggtagacacc cgcggttacc atcgttgcgg cgtggatcag cgcggagaca
     5821 ggcgtcgggc ccgccatcgc gtcagcaagc catgtctgca acggcaactg cgcagattta
     5881 ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt gccgtcagca
     5941 aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag ttcgttgtaa
     6001 agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac gaacgctttc
     6061 attgccgctg cgccattctt cggatcggtg taatagaaac cgatcagcag ataggagcac
     6121 aggcccacgc cttcccagcc gaggtacatc agcagcaggt tatcggcgag aaccagaact
     6181 accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc ctcttcaccg
     6241 cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac caccgagagc
     6301 atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac
     6361 atccacgtcc acagcggctg gctgtatgac tgctcgccgt tagcgaagaa atcaacgccg
     6421 ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat cgccgaaacg
     6481 ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa tggcaaaata
     6541 atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg ttcaggttct
     6601 ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct gccgccgcga
     6661 ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag cttccggcga
     6721 ccacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc ataaacagca
     6781 gattgcgacg gataaccaga ccggttaagc caagaacgaa taagattgcc gcgaggatca
     6841 gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt
     6901 acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag
     6961 acccgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc cgaacagcgt
     7021 aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcat taacaccaag
     7081 gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccggac caatccacac
     7141 ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt tgagcatcat
     7201 caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt ccagcgcacc
     7261 ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc gccagcagcg aaataatcag
     7321 gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg caagtatggc
     7381 tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg taacaggctc
     7441 ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc gtcgattgcc
     7501 atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga gatcagcaga
     7561 tcctcttttt cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa atccggggtt
     7621 aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat gcagcgtgag
     7681 aagttgatgc ggaaaaattc cgggtaccag cgaccgtctt tggtttctgc tttttgcagc
     7741 gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca acgctcttcg
     7801 ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag atagaccggc
     7861 tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat catccagata
     7921 ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt cttattgccc
     7981 cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc agcgtcagcg
     8041 gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc ggtaacgacg
     8101 cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac cagatgaatg
     8161 gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat gcagagatgg
     8221 tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg gaatattcaa
     8281 tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg tgacggtgac
     8341 ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg ataacgttcc
     8401 acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca
     8461 cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca gaagcacgca
     8521 tcgcacccag caacgagtat ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa
     8581 ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc
     8641 aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag gcaatcatcg
     8701 gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct ttaaagaaca
     8761 ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg ttaggtccgt
     8821 aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat gccccgcagg
     8881 tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc tcaatcagtt
     8941 ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg cgccagccag
     9001 caccggagca atgccggaca tacccatcgg caaacccacc tgccctgccg tcagcccttc
     9061 agcgatttca accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga cgcgagcgcc
     9121 tgcgttcacg cccaacttcg cggcatccgc tgggttgagt ttgatgtacg gctgcggcat
     9181 acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca ggtggtaata
     9241 cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta
     9301 atccagacca ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa agcgcagttt
     9361 gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc ccggcgccca
     9421 ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg ttaccttcca tcgagaaggt
     9481 gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat tggcgcgcat
     9541 ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac gaatacggaa
     9601 tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc gggattttcg ccacaacagc
     9661 gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg tgctgtgcag
     9721 cgagtgtaac cagcgccagc tttccagcat gacagttttg ctgtcgtaat aagcaggatc
     9781 gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac cgtcgctttc
     9841 agcaaagctg gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg tgcgttgatg
     9901 atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg tagcggaagc
     9961 gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg tttccagttc
    10021 ggtcaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc tgttgacgga
    10081 acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt tcgcgacgtt
    10141 agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa tcaacggttt
    10201 cttcgcacct gccagtgcct gcacgatgac gtcgattttg ctttgcagct caggttcgat
    10261 accgtcaacc gctggcgcag agttatccag cgcatgggcg atggcaaaac ctaaacgcgc
    10321 ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac gggtgtcatc
    10381 aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga ttgccgcaat
    10441 ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt tcacagcctg
    10501 acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac
    10561 cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt cacgcagcac
    10621 tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt agaagttttc
    10681 ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat
    10741 accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg cctgttcggc
    10801 gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac gatccttcag
    10861 gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc
    10921 gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc tgatgttaca
    10981 gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt tacggttgta
    11041 acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga ccaggttacc
    11101 ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt tgtcgtgcgc
    11161 accgtaaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca cacagcggta
    11221 gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct gattacggtg
    11281 ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg tcatatcctg
    11341 aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt tggtcattaa
    11401 ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt
    11461 gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt cttccgcgtt
    11521 ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg ccggatgcca
    11581 gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt tgtccgctcc
    11641 gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc ttccagtttt
    11701 ctcagtcaga gactgagcgt taatcgaaat tcggttacca gcgctctttc agcaggttcg
    11761 gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc gcctcaaatt
    11821 cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt gcgtgggcac
    11881 agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg atatcgcccg
    11941 gctgaccttc accacgctcc agcgcacgca gaattttcac gctccacggc agaccgtcgc
    12001 ggcacggcgt acaccagccg caggattcac gggcgaaaaa ctcttccagg ttacgcacca
    12061 gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc agacggcttc
    12121 ctgctttacc gatactttcg aattccatcg gcaggtcaag atgcgcttcg gtcaggaagt
    12181 cggtgcccgc gccgcctggc tgccaggctt tgaatttcag accgtcacgc ataccaccgg
    12241 cgtaatcttc gaggatctcg cgcgcggtag taccaaacgg cagttcccac aggcccggat
    12301 ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt ttcgagatgt
    12361 tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg gtttcgacgt
    12421 tgttgacaca ggtcggttta ccccatgcgc cggaggtcgc cgggaatgga ggcttcgagc
    12481 gtgggttagc acgacgtcct tccagggagt tgattaacgc tgtttcttcc ccgcagatgt
    12541 agcgccctgc cccggtatgg acgaacagtt cgaagtcaaa acctgttccc ataatatttt
    12601 tgccaagcag acctgcttcg gtggcttcgg caatggcacg gcgtagattc actgccgctt
    12661 cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca aacgcggaga
    12721 tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg tctttatagg
    12781 tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg gattcgtctt
    12841 tcggcatcag gctccacttc aggccagtcg aaaagcccgc gccgccgcgc cctttcagac
    12901 cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc gccttacgcg
    12961 ccccttcgta accatttttg ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc
    13021 gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc atttataccg
    13081 ctccagcagt tcagggatcg cttccggggt caaatgcgcg tgagtgtcct catcgatcat
    13141 catatttggc cctttatcac agttccccag gcagcaggtt ggcagcagcg taaagcgacc
    13201 gtcaaacgtc gtttgccctg gtttgatgtt cagttttttc tccagcgccg cctgaatacc
    13261 ctggtaaccg ttgatgtgac agaccacgct gtcgcaataa cggatcacat ggcgaccaac
    13321 cggttggcgg aagatctggc tatagaacgt tgccacgcct tcgacatcgc tcgccggaat
    13381 acccagcaca tcggcgatcg cgtggatcgc gccgtccggc acccagccac gctgcttctg
    13441 aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg
    13501 ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt
    13561 ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc cagataaaca
    13621 atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa atgcgcataa
    13681 ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt caggtagtaa
    13741 ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg catcaccggc
    13801 ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag cgtgcgctct
    13861 ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg catgttgttg
    13921 aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag cattacgcgg
    13981 gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt ttcatagcca
    14041 gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca
    14101 gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg ggaacgacct
    14161 ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt cggcatccag
    14221 tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca
    14281 atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag atcgtaaatt
    14341 ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag
    14401 gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat aacgcgaatg
    14461 acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag cacgtaaggc
    14521 atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat gtagctgtgc
    14581 caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat gtctggtacg
    14641 cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc gtgcgccgac
    14701 gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc gcgcttcatc
    14761 ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt ggctttggtc
    14821 agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag cgggtgacct
    14881 ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa agtaatgcca
    14941 aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc
    15001 ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg gttacgatcg
    15061 atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc gcggtgtgtg
    15121 cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt cggcagtttc
    15181 tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac gggaaccccg
    15241 gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt
    15301 accggatcat caagatgatc gcgggtctgc caggcgggtt cttgcgcggt taagtcggtc
    15361 atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc ttcgcgaaat
    15421 agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta aatctcgtca
    15481 ggtgtacgca ggttggttac ggcaatacgt tcaccgcgct tgcgttcgcg ctctgattgc
    15541 atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg acgttctttg
    15601 ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg cggcgggcag
    15661 cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac ggaataaata
    15721 tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt tggttccagc
    15781 atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt tcctgcaacc
    15841 accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg
    15901 tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag accgaagtta
    15961 tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt gcccataaac
    16021 acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt ttgcaggggg
    16081 taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat cttaatgcct
    16141 cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt
    16201 gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag acctgccagt
    16261 aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc gcggatagag
    16321 gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa catggccacc
    16381 agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat accggattca
    16441 aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc
    16501 atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca gtgatgagcg
    16561 atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc gccagattca
    16621 ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa aaaaacctta
    16681 caatcactgt agaaattctt ttatacagct aattgatgtg gtcttttact cctttctata
    16741 accttttgtc aactttaaca aaagtttctt cacattagtt tacataatat caacaccatt
    16801 agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta gatgttgttg
    16861 aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag gaatttgtgt
    16921 cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt gtcattccat
    16981 aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag gtgatttttt
    17041 tcaggcgatt atttgtgcgt tcgggacgtg aatctctggt gattgaaaaa tgaacagttt
    17101 tgtacgttct gcactatgcg aggaaggatt tttactaaaa aaaagccgct ggggtttaaa
    17161 acacccccag cggctcgttt tttacactat tgtctcagga attatctatc gtccgtcgat
    17221 tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga cattgggctg
    17281 tattgccacg gattatggta gctctccatc gcctgataga tcacctgggc cagctcatta
    17341 ttactggacg ggtcatagca cagcaaatat tcggtatcag gtagcggcgg taagccatct
    17401 actccactca gcacacgcag gtccgggctc atcatctcaa ccggccttgc cgtcacgcca
    17461 agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata agccagtcgc
    17521 catggaatat ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg acttggatca
    17581 tccagcaata caagagggat cggctcgcct ttttgcaata cgtattccgc tgcgcagtac
    17641 cagtgtgttg gcgaggttcg caggttcaat gccttaaacg ctgaaggacg atgggtggtt
    17701 accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt acgtttaacg
    17761 cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag gaaaggtaag
    17821 atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac
    17881 attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta accaagaagt
    17941 tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca
    18001 acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg
    18061 gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct cagcagatcg
    18121 aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt actggcggct
    18181 catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct
    18241 ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa
    18301 aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat atcttcaggg
    18361 atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt gtttgtataa
    18421 atgtaaatgt gagtccttgt tccactctcg tgcagcatcg ctggtcatac gcgaacacgt
    18481 acaaacagca atggtgaggc ggcatcaaga gcagggatcc gtcatttatg cgagcatttt
    18541 accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa agttatgtta
    18601 tgttaggcaa agtaatcttc tattattaat aagcacatca aaaccttttt gaatattaaa
    18661 taataattaa ttagcatcat cctcattcat taattccgct taaca
//
LOCUS       DAZKGS010000080        18409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKGS010000080 DAZKGS010000000
VERSION     DAZKGS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..637)
                     /gene="rsmG"
                     /locus_tag="ACHOQY_002839"
     CDS             complement(14..637)
                     /gene="rsmG"
                     /locus_tag="ACHOQY_002839"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9678901.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(701..2590)
                     /gene="mnmG"
                     /locus_tag="ACHOQY_002840"
     CDS             complement(701..2590)
                     /gene="mnmG"
                     /locus_tag="ACHOQY_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9678902.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2968..3411)
                     /gene="mioC"
                     /locus_tag="ACHOQY_002841"
     CDS             complement(2968..3411)
                     /gene="mioC"
                     /locus_tag="ACHOQY_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9678903.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3501..3959)
                     /gene="asnC"
                     /locus_tag="ACHOQY_002842"
     CDS             complement(3501..3959)
                     /gene="asnC"
                     /locus_tag="ACHOQY_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9678904.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4111..5103
                     /gene="asnA"
                     /locus_tag="ACHOQY_002843"
     CDS             4111..5103
                     /gene="asnA"
                     /locus_tag="ACHOQY_002843"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9678905.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5108..6559)
                     /gene="viaA"
                     /locus_tag="ACHOQY_002844"
     CDS             complement(5108..6559)
                     /gene="viaA"
                     /locus_tag="ACHOQY_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9678906.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6553..8049)
                     /gene="ravA"
                     /locus_tag="ACHOQY_002845"
     CDS             complement(6553..8049)
                     /gene="ravA"
                     /locus_tag="ACHOQY_002845"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9678907.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8272..10140
                     /gene="kup"
                     /locus_tag="ACHOQY_002846"
     CDS             8272..10140
                     /gene="kup"
                     /locus_tag="ACHOQY_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9678908.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10307..10726
                     /gene="rbsD"
                     /locus_tag="ACHOQY_002847"
     CDS             10307..10726
                     /gene="rbsD"
                     /locus_tag="ACHOQY_002847"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9678909.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10734..12239
                     /gene="rbsA"
                     /locus_tag="ACHOQY_002848"
     CDS             10734..12239
                     /gene="rbsA"
                     /locus_tag="ACHOQY_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9678910.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12244..13209
                     /gene="rbsC"
                     /locus_tag="ACHOQY_002849"
     CDS             12244..13209
                     /gene="rbsC"
                     /locus_tag="ACHOQY_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9678911.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13234..14124
                     /gene="rbsB"
                     /locus_tag="ACHOQY_002850"
     CDS             13234..14124
                     /gene="rbsB"
                     /locus_tag="ACHOQY_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9678912.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14250..15179
                     /gene="rbsK"
                     /locus_tag="ACHOQY_002851"
     CDS             14250..15179
                     /gene="rbsK"
                     /locus_tag="ACHOQY_002851"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9678913.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15183..16175
                     /gene="rbsR"
                     /locus_tag="ACHOQY_002852"
     CDS             15183..16175
                     /gene="rbsR"
                     /locus_tag="ACHOQY_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9678914.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16141..17568)
                     /gene="mdtD"
                     /locus_tag="ACHOQY_002853"
     CDS             complement(16141..17568)
                     /gene="mdtD"
                     /locus_tag="ACHOQY_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9678915.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17591..18283)
                     /gene="yieP"
                     /locus_tag="ACHOQY_002854"
     CDS             complement(17591..18283)
                     /gene="yieP"
                     /locus_tag="ACHOQY_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9678916.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4437 a         4559 c         4802 g         4611 t
ORIGIN      
        1 tttttgataa aaattaaatt ttatttgctt taatcaccac cagatgacgt tcgccatcca
       61 aggctggaac ctgaagttta accactgatt cgacctgata ttcttcgggc aacaaagcga
      121 tttcatcttc cggcatttgc cctttcagcg cgtagaaacg gccttgctca ccaggaagat
      181 ggtggcacca gctcaccata tcgttcagag aggcaaaagc gcggctaatt acgccatcaa
      241 atggcggctc tgaaggaaac tcttctaccc tgctctgtac tggttcaata ttctccagtt
      301 taagctcatg ttgcacctga cgaaggaaac gcacgcgttt accaaggcta tccaacagag
      361 tgaaatgggc ttcaggacgc acgatagaga gtggaatgcc tggcagtcct ggtccggtgc
      421 cgacatcgat aaaccgttca ccttgcagat acggtgccac cacaatgcta tcgagaatat
      481 ggcgtaccag catctcatta ggatcgcgga ccgaagtcag gttgtacgct ttgttccatt
      541 tatgcagcat attcacgtag gcaataagct ggtttttctg gtgatcggta agcgaaatac
      601 ctgcgtcttt cagcagtaag gagagtttgt tgagcacggt gattacctgt tcttgatgcg
      661 ttgcctggta agcgggtgct taccaggcat ttttaatgcg ttatgcgcta cgacgcagca
      721 taccctgttt tttcagccac accagcagaa tggagatggc cgcaggcgtg acgccagaaa
      781 tacgcgaagc ctggccgata gaggctggtt tgtgatcgtt aagtttggcg atcacttcgt
      841 tagaaagacc ggatacctgg cggtaatcca gtgtcgcggg tagcagggtg ttctcgttac
      901 gcagctgctt ttcgatctca tcttgctggc gcgcgatata accttcgtat ttaacctgaa
      961 tctcaacctg ttccgccgcc tgttcgtctg tcaacgcagg ggcaaacggc gtcagcgtgg
     1021 ttaatttttc ataagtcatt tccggacgac gcagcagatc ttcaccactg gcttcacggg
     1081 aaagcggcgc agtcaggtga gcattcactt cggctgcagc ttccgccgac ggggttaccc
     1141 aggtcgattt cagacgctga cgctcacgct cgatattctc aagtttctcg ttaaagcgcg
     1201 cccaacgttc gtcatccacc aggcccagtt cacgaccgat ttcagtcaaa cgcagatccg
     1261 cattatcttc gcgtagcatc agacgatatt ctgcgcgcga agtaaacata cgatacggtt
     1321 ctttggttcc taaagtgcac aggtcatcaa ctagtacgcc gagatacgcc tgagaacgtg
     1381 ccggagccca accttctttg tcagcagaca gacgggcagc gttaagaccg gccagcaaac
     1441 cttgcgcagc ggcttcttcg taaccggtag tgccgttaat ctgaccagca aagaacagcc
     1501 cctggataaa cttgctctcc agcgtcggtt tcaggtcgcg aggatcgaag aagtcatact
     1561 caatggcata acccggacgc acgatcttcg cgttttccat cccctgcata gagcggacga
     1621 tttgcatctg cacatcgaac ggcaggctgg tggagatacc gttcggataa atttcattag
     1681 aggtcagtcc ttccggttca aggaagatct gatgctgatt tctgtcggcg aagcgcataa
     1741 ctttgtcttc gatcgacggg cagtagcgtg ggccgacacc ttcgatcacc cctgcataca
     1801 ttgggctacg atcgaggtta ctgcggatca catcatgggt tttctcgttg gtatgagtga
     1861 tataacacgg cacctgctgg ggatgctggg acgcattgcc cataaacgag aataccggca
     1921 ttgggttatc gccatgctgt tgcgccagca cactaaagtc gatagttcga gcatcaatac
     1981 gcggtggtgt cccggttttc agacgaccaa cgcgcagcgg cagttcacgc aaacggcgag
     2041 aaagcggaat ggacggcgga tcaccagcac ggccaccgct gtaattatcc agaccgatat
     2101 gaattttacc gtcgaggaac gtcccaacgg tgagcacgac ggctttggca cggaacttca
     2161 gtcccatttg ggtaacagca ccgaccacgc gatcgttttc gacaataaga tcttcaaccg
     2221 cctgctggaa gatcatcagg ttcggttggt tctccagcgc cgtacgtacc gcctgacggt
     2281 agagcacacg atccgcctga gctcgggtag cgcgaaccgc cggtcctttg cttgcgttta
     2341 gtatcctaaa ctggataccc gcttgatcga tcgctttcgc catcagaccg ccgagtgcat
     2401 ccacttcttt taccagatgt cccttcccaa taccgccgat cgccgggttg cagctcatct
     2461 gccccagagt gtcgatattg tgtgtcaaaa gcagagtctg ttgacccata cgcgccgcgg
     2521 ccatcgcggc ctcggtgcct gcatgacccc cgccaatgat gatgacgtca aaaggatccg
     2581 gataaaacat ggtgattgcc tcgcataacg cggtatgaaa atggattgaa gcccgggccg
     2641 tggattctac tcaactttgt cggcttgaga aagacctggg atcctgggta ttaaaaagaa
     2701 gatctattta tttagagatc tgttctattg tgatctctta ttaggatcgc actccctgtg
     2761 gataacaagg atccggcttt taagatcaac aaccgggaaa ggatcattaa ctgtgaatga
     2821 tcggtgatcc tggaccgtat aagctgggat cagaatgagg ggttatacac aactcaaaaa
     2881 ctgaacaacc gttgttcctt ggataactac cggttgatcc aagcttcctg acagagttat
     2941 ccacagtaga tcgcacgatc tgtatactta tttgagtaaa ttaatccacg atcccagcca
     3001 ttcttctgcc ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc
     3061 tgtctgtttt gcaccggaat ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa
     3121 ggtgtcatat tcacgactac caataccgat tgcgccaaag cggaccgcag aaagatcggg
     3181 cttctgttcc tgcaatgctt catagaaagg agaaaggttg tccggaatat ctccggcacc
     3241 gtgggtggag ctgataatca gccagatccc cgaggcagat aaatcttcca acagcggacc
     3301 gtgcagcgtt tcggtggtaa aacccgcctc ttccagcttt tcagccaggt gttctgctac
     3361 atattcggca ccgccgaggg tgctgccgct gataagagtg atatctgcca taaaccgcca
     3421 cctttattaa gagtggcgta ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag
     3481 tatgggatta aaaaagccga ttatggcttg atggtacgca tgatcgggtt ttgcaggacg
     3541 atcaatgtct cggtggactg aatttcatca attgtttgga tcttgttgat aagtacatgc
     3601 tggagagcgt cgatcgaacg gcacatcact tttataaaga tgctgtagtg gccggttgtg
     3661 taataggctt cagtgacttc atcaaggctt tccagctttg ccagcgcgga agggtagtct
     3721 ttggcgctct ttaatataat gccgataaag cagcctacgt cataaccgag ctgcttcggg
     3781 ctgacatcaa tacgcgcccc ggtaatgatc cccgcctgct tcattttctc tactcgaacg
     3841 tgaatcgtcc ccggactgac gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca
     3901 ttgcccatta atgcttccag gatgccacgg tccagattgt cgatcagata attttccata
     3961 ggattttctt atgcagattg atgattcatt ctattttagc cttctttttt aatgaatcaa
     4021 aagtgtgtta ggctttttat tgaatgatta ttgcatgtgt gtcggttttt gttgcttaat
     4081 cataagcaac aggacgcagg agtataaaaa atgaaaaccg cttacattgc caaacaacgc
     4141 caaattagct tcgtgaaatc tcacttttct cgccaactgg aagaacgtct ggggctgatc
     4201 gaagtccagg cgccgattct tagccgtgtg ggggatggca cgcaggataa cttgtcgggc
     4261 tgtgaaaaag cggtgcaggt aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt
     4321 cattcactgg cgaagtggaa acgtcagacc ttagggcaac acgacttcag cgcgggcgaa
     4381 gggctgtaca cgcacatgaa agcccttcgc cccgatgaag agcgtctttc tccgttgcac
     4441 tcggtctatg ttgaccagtg ggactgggaa cgcgtaatgg gcgacggtga gcgtcaattc
     4501 tcgactctga aaagcacggt agaggcgatc tgggcgggaa ttaaagcaac cgaagctgca
     4561 gttagcgaag agtttggttt ggcaccgttc ctgccggatc agatccactt cgtacacagc
     4621 caggagttac tgtctcgtta tccggatctc gatgccaaag ggcgtgagcg ggcgattgcg
     4681 aaagatcttg gcgcggtatt cctcgtcggg attggcggca agctgagtga tggtcatcgt
     4741 cacgacgtgc gcgcaccgga ttatgatgac tggagcaccc cgtcagagct gggccatgcg
     4801 ggtctgaacg gcgatattct ggtatggaac cctgtactgg aagatgcgtt tgagctttct
     4861 tccatgggga tccgcgtgga tgccgacacg ttgaagcatc agttggcgct gaccggtgat
     4921 gaagatcgtc tgcagctgga atggcatcag gcgctgctgc gcggtgaaat gccgcagacc
     4981 atcggcggcg gtatcggcca gtctcgtttg accatgctgc tgctgcaact gccgcatatc
     5041 ggccaggttc agtgtggagt atggccagct gctgttcgcg agagcgtccc ttctctgctg
     5101 taataattta tcgccgccag cgtctgagca ggcggcttcg catcccggta tcaaagcgcc
     5161 agatatgatc gaaaatgcgc atgatgccgg gtttgccgtg tgccgacatc gccacggcat
     5221 gaaagcgatg ctgatgtacc cgctgcagct ctttcacttt actcgtcacg tcgtcaggca
     5281 accgctgagc gataaaatca gaaatcacca ccgcatcggc atcaaaccat tccctgcttt
     5341 gcaagcgttc cataatggcg cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct
     5401 ggcttaaaaa acggattgct tgttcgatgc cttgtgggcc tgaaagctca taacggacga
     5461 tctcggtgga aaatagcata atatagcagc gccggttttc agcgagagca atgcgcatca
     5521 aggccaggca gaacgctttc gcacactgtt cattaaagcc gcccattgag ccggaagtat
     5581 ccacacagac aataaacggc ccgcgcggct gttcatcgta atctttatgt accaccggac
     5641 gttcgatcac tttttcacgc cacgactcac cgtgcaggcg ataggtgagc aactgttttt
     5701 ccaccagccg acggtaaaac tcatactcca gttccgttat ccctagtgtc gccagttctg
     5761 gcggcagaag acgtaaaata tcatcgcttt gttgcagacc atcaacctgc tcaggaaccg
     5821 tcgccggttc gcgcaccatg gtgcggaagg tttccatctg cgcatcgttg cgcggtattg
     5881 atttggcttc ccgagaacgc cccagctgct ctgccaggcg tttcagttcc ggctgttcgt
     5941 taagaaattc accgtatttc acaatcaact gatagtcgcc acgtttaagc tgaccggcgc
     6001 tcatatccca cagacgacca gctgcagtat tgttatctgc gagaatcggt tcaagttgtc
     6061 cgctcagcgt catgcgttcc tgaacttcac tcaacagttg ttcgcgttct tcttctaata
     6121 gctgttgatt taacgtcgtt gcttgcacga tcagacttaa acgccaacgc tggagaaaaa
     6181 gcgtgtgtaa cgctgaagtg atcgtgctgt tagcatcaac caactgtcgg gcttgttctg
     6241 cccatggaga attcagacga tgcagtaagt ccaggatctg tggtagctgc acaataaact
     6301 gtggcgtgga gaggagctgg ctttgctgat agcacatcac ctcttcggtg agttccggcg
     6361 ggactcgggc atctttcagc cgactgcgca gcgcttcacg ccagcgggga acatcatcag
     6421 tgattgccgc cttcaggcgt gggaattttt caaagaagac cgccagctgc ggtgaggcca
     6481 gcagtgcgat gatcatctct tcgatcaatc cctcttcgct gacggccagc atcacattaa
     6541 gcgtatccag cgttagcatt gttgtgcctg gcgaatctgt gcaccgacat cctgtaggct
     6601 ggcttcaatg cgacctaacc agtcgccagg gataaacaga catttttgct gttcgctgaa
     6661 taaagcgtgt tgcttgcgcc agtcgctttc aagctcttcc agttgttgtt ttatttcacc
     6721 aggcagacct tcagccgatg aaccgggaag tgccagcgta ctgccttgta aacttacatc
     6781 gcgaacgaca agatgttggg cgctatcaac ttccagattc agtttctggg caaagccgat
     6841 accgttcagt ttaccgcgaa tttcaccacc tttgctcagc cactgttcca gcgcgctacg
     6901 ctcaaaggag atatgaacca cttccatatc atgcagtttt aacggttttt gcagcagtag
     6961 agtcagagtg gaagcagtaa cgttaacagg aagttgatac tgctgacgac ggctgaaaat
     7021 gccgcccaga cgaattaccg ttaaggctgt tttatcgctt tgttgctgct ggagttgcag
     7081 gtgacgttgc acaatcgcgc caaggcgagt caacatccct tgctgttgcc aggcgtgacc
     7141 ggtcatcaat acatcaattt gttgttgtat caaattcagg ctttgcgcgt cataccacag
     7201 gcaatctttc agcaaaatga gatctaccgg ggcaacagca ctgcgaccgc taaaaaaggc
     7261 gctggcctgc aataatcgga tcgctttttt ccagcgacga tccgagacat aaggcgcatc
     7321 cggtaattta tccagttgct ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag
     7381 cgtaatttca ccaatctctt tctgccagcg ttcatattct tcatctgtga cctgcaaggc
     7441 atcaggaacc ggattgtcgt tttcatcctg ttgactggtc agcatggagc ggaaattcgc
     7501 tttatcctgc actttatcta accacagacg aatcagcatg cggtcatata acgcttccag
     7561 actgctgtct gcttccggca gctcgttgga ggccgccacc agcagacgca tcgggatttt
     7621 ttctacgtgt gcaccgttgc ggaactggcg ctcgttaatg gcggtgagca aggtattaag
     7681 aattgccggg cccgctttcc agatctcatc cagaaagaca atttctgctt ccggcaggta
     7741 accgctggtt aaacgttcat agcgcccttc atcttttagc gcctgaatag aaaggggacc
     7801 aaaaacttct tccggcgtgg agaagcgggt catcagatat tcaaacgcgc gggcattctg
     7861 aaaggcgaat tttaagcgcc gggcgatcaa acttttggca atacctggcg ggccaaggag
     7921 gaacacactt tcaccactta atgccgctaa taaacacaag cggatggcgt ggctacgttc
     7981 ataaagcccc ttttccaacg aactgctcag gcgggaaatt ctttccgcta ataaatgagg
     8041 gtgagccata atgaagtggc gtcctttcgt caaaagttct gcgtaaattg cgagtataga
     8101 cgtttcttgc tggtggctaa aatagtctca aagggggggt atttttcttt gagccaggtt
     8161 aatgtggccg catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt
     8221 gtgggccaag ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact
     8281 gataataagc aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat
     8341 attggtacca gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc
     8401 gttgaacgcg ataccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg
     8461 gtttccatta aatatctcac cttcgtgatg cgggcagata acgccggtga gggggggatc
     8521 ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg
     8581 attatggggc taatcggcgg cagctttttc tatggtgaag tcgtcataac acccgctatt
     8641 tcggtgatgt cagccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata
     8701 gttcccctct caattatcgt tctcacttta ttatttatga ttcaaaaaca tggcacggct
     8761 atggtcggta agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcaggactg
     8821 gggttacgta gcattattgc taacccggaa gtgctgcatg cgctgaatcc aatgtgggcg
     8881 gtgcatttct tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgttg
     8941 tcgattacgg gggtcgaggc gctgtatgct gatatggggc actttggtaa gttccctatt
     9001 cgcctggcgt ggttcaccgt cgtattacct tccttgaccc ttaattactt cggtcaggga
     9061 gcgctgttgt taaagaaccc ggaagcgatt aagaacccgt tcttcctgtt ggcaccggac
     9121 tgggcgctga tcccgctgct gatcatcgcc gcactggcga cggtaattgc ctcgcaggcg
     9181 gttatctctg gcgtcttctc attgacgcgt caggcggtac gtctgggata tttgtcgccg
     9241 atgcgcatta ttcacacctc cgaaatggag tcagggcaaa tctatattcc gtttgtgaac
     9301 tggatgctct atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg
     9361 gcggcggcgt acgggattgc ggtgaccgga accatggtgc tgacgtctat tctctcgact
     9421 accgtggcac gtcagaactg gcactggaat aagtattttg ttgcgctgat cctgattgct
     9481 ttcctttgtg tcgatattcc attgttcacc gctaacctcg ataaactgct ctccggcggc
     9541 tggttgccat tgagcctcgg tactgtgatg tttatcgtga tgaccacctg gaagagcgag
     9601 cgtttccgct tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct
     9661 tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg cggtgtatat gtcgcgtgca
     9721 atcaacgtca ttccctttgc gctgatgcat aaccttaaac ataacaaggt attgcatgag
     9781 cgggtgattc tgttaactct gcgtaccgaa gacgcgccat atgtccataa tgtccgtcgg
     9841 gtacagattg aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga
     9901 gaaacgccaa acgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg
     9961 atgatggaaa cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg
    10021 tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca
    10081 gatcaatttg aaattccgcc aaacagggtt atcgaactgg gtactcaggt cgaaatctaa
    10141 cgccagacgc ctcctttctc cataaggggg cgtttttgtt ttcatggtta atcaccatgt
    10201 aaaacgtttc gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca
    10261 ggattaaact gcgggtcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac
    10321 cgttcttaat tctgatattt catcggtgat ctcccgtctg ggacataccg atacgctggt
    10381 ggtgtgtgat gctggtttac ctatccccaa aagtacaacg cgtatcgata tggcattaac
    10441 ccagggtgta ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga
    10501 ggctgccatt atcgcggaag agatcaaaca acataatccg caactccacg aaacgttgct
    10561 cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac
    10621 gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg
    10681 ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag
    10741 cattacttca gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg
    10801 cagcgttaaa tgtctatccg ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta
    10861 aatccaccat gatgaaagtg cttactggta tctatgctcg cgatgccggc acgcttttat
    10921 ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga
    10981 ttatccatca ggaactgaac ctgatcccgc agttgaccat tgccgaaaac attttcctcg
    11041 gtcgtgagtt tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg
    11101 ataaattgct ggctaaactt aacctgcgct ttaaaagcga caagctggtg ggcgatcttt
    11161 ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct gagctttgag tcgaaagtca
    11221 tcattatgga tgaaccgacc gatgcgctga ccgataccga aaccgaatcc ctgttccgcg
    11281 tcatccgcga actaaaatcg caaggccgcg gtattgtcta tatctcccac cgcatgaaag
    11341 aaatcttcga gatttgcgat gacgttaccg tttttcgtga cgggcaattt attgctgagc
    11401 gcgaagtggc atcactgacc gaagattcgc tgattgagat gatggtgggc cgcaagctgg
    11461 aagatcaata tccacacctg gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc
    11521 tctgcggacc tggcgttaac gatgtctctt ttactttacg caaaggcgaa attcttggcg
    11581 tctctggttt gatgggcgca ggtcgtaccg aactgatgaa agtgctctac ggcgcactac
    11641 cgcgcaccag cggttacgtc accctggatg ggcatgaagt cgttacccgt tcaccgcagg
    11701 atggcctggc aaacggcatt gtgtatatct ccgaagaccg taaacgtgac ggtttagtgt
    11761 tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct gcgctacttc agccgcgctg
    11821 gcggcagttt gaagcatgcc gatgaacagc aggctgtgag tgatttcatt cgtctgttta
    11881 atgtgaaaac accgtcgatg gaacaggcaa ttggtctgct ttccggtggc aatcagcaaa
    11941 aagtggcgat tgcccgcggt ctgatgacac gccccaaagt gttgatcctt gatgaaccta
    12001 cccgtggcgt agatgtcggc gcgaaaaaag agatctatca actgattaac cagttcaaag
    12061 ccgatggctt gagcatcatt ctggtgtcat cggagatgcc agaagtatta ggcatgagcg
    12121 atcgcatcat cgtcatgcat gaagggcatc tcagcgggga atttactcgt gagcaggcca
    12181 cccaggaagt gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa
    12241 aaaatgacaa cccagactgt ctctggtcgc cgttatttca cgaaagcgtg gctgatggag
    12301 cagaaatcgc ttatcgctct gctggtgctg atcgcgattg tctcgacgtt aagcccgaac
    12361 tttttcacca tcaataactt attcaatatt ctccagcaaa cctcggtgaa cgccattatg
    12421 gcggtcggga tgacgctggt gatcctgacg tcgggcatcg acttgtctgt aggttctctg
    12481 ttggcgctaa ccggcgcagt tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg
    12541 gctgtcgctg ccgctctcgc gttaggtgcc gcaattggtg cggtaaccgg ggtgattgta
    12601 gcgaaaggtc gcgttcaggc gtttatcgct acgctggtta tgatgctttt actgcgcggc
    12661 gtgaccatgg tttataccaa cggtagccca gtgaataccg gctttactga gaacgccgat
    12721 ctgtttggct ggtttggtat tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg
    12781 gggattgtct tcctcgcggc ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc
    12841 tacgcgctgg gcggcaacga agcggcaacg cgtctttctg gtatcaacgt caataaaatc
    12901 aaaatcatcg tctattctct ttgtggtctg ctggcatcgc tggccgggat cattgaagtg
    12961 gcgcgtctct cctccgcaca acccacggcg gggactggct atgagctgga tgctattgct
    13021 gcggtggttc tgggcggtac gagtctggcg ggcggaaaag gtcgcattgt tgggacgttg
    13081 atcggcgcat taattcttgg cttccttaat aatggattga atttgttagg tgtttcctcc
    13141 tattaccaga tgatcgtcaa agcggtggtg attttgctgg cggtgctggt agacaacaaa
    13201 aagcagtaat aacgactaca ggacatcttg aatatgaaca tgaaaaaact ggctaccctg
    13261 gtttccgctg ttgcgctaag cgccaccgtc agtgcgaatg cgatggcaaa agacaccatc
    13321 gcgctggtgg tctccacgct taacaacccg ttctttgtat cgctgaaaga tggcgcgcag
    13381 aaagaggcgg ataagcttgg ctataacctg gtggtgctgg actcccagaa caacccggcg
    13441 aaagagctgg cgaacgtgca ggacttaacc gttcgcggca ccaaaattct gctgattaac
    13501 ccgaccgact ccgacgcagt aggtaatgct gtgaagatgg ctaaccaggc gaacatcccg
    13561 gttatcactc ttgaccgcca ggcaacgaaa ggtgaagtgg tgagccacat tgcttctgat
    13621 aacgtactgg gtggcaaaat cgccggtgat tacatcgcga agaaagcggg tgaaggtgcc
    13681 aaagttatcg agctgcaagg cattgctggt acatccgcag cccgtgaacg tggcgaaggc
    13741 ttccagcagg ccgttgctgc tcacaagttt aatgttcttg ccagccagcc agcagatttt
    13801 gatcgcacta aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag
    13861 gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcgcgcact gcaaactgcc
    13921 ggtaaatcgg atgtgatggt cgtcggattt gacggtacac cggatggcga aaaagcggtg
    13981 aatgatggca aactagcagc gactatcgct cagctacccg atcagattgg cgcgaaaggc
    14041 gtcgaaaccg cagataaagt gctgaaaggc gagaaagttc aggctaagta tccggttgat
    14101 ctgaaactgg ttgttaagca gtagttttaa tcaggttgta tgacctgatg gtgacataaa
    14161 tacgtcatcg acagatgaac gtgtaatata aagaaaagca gggcacgcgc caccctaaca
    14221 cggtggcgca ttttatggac atcccgaata tgcaaaacgc aggcagcctc gttgttcttg
    14281 gcagcattaa tgctgaccac attcttaatc ttcaatcttt tcctactcca ggcgaaaccg
    14341 taaccggtaa ccactatcag gttgcatttg gcggcaaagg cgcgaatcag gctgtggctg
    14401 ctgggcgtag cggtgcgaat atcgcgttta ttgcctgtac gggtgatgac agcattggtg
    14461 agagcgttcg ccagcagctc gccactgata acattgatat tactccggtc agcgtgatca
    14521 aaggcgaatc aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag aatgtcatcg
    14581 gtattcatgc cggcgctaat gctgcccttt ccccggcgct ggtggaagcg caacgtgagc
    14641 gtattgccaa cgcgtcagca ttattaatgc agctggaatc accactcgaa agtgtgatgg
    14701 cagcggcgaa aatcgcccat caaaataaga ctatcgttgc gcttaacccg gctccggctc
    14761 gcgaacttcc tgacgaactg ctggcgctgg tggacattat tacgccaaac gaaacggaag
    14821 cagaaaagct caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag gcggcgcagg
    14881 tactgcatga aaaaggtatc cgtactgtac tgattacttt aggaagtcgt ggtgtatggg
    14941 ctagcgtgaa tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct gtcgatacca
    15001 ttgctgccgg agataccttt aacggcgcgt taatcacggc gttgctggaa gaaaaaccat
    15061 tgccagaggc gattcggttt gcccatgctg ccgctgcgat tgccgtaaca cgtaaaggcg
    15121 cacaaccttc cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg cagaggtgac
    15181 gcttggctac aatgaaagat gttgcccgcc tggcgggcgt ttctacctca acagtttctc
    15241 acgttatcaa taaagatcgc ttcgtcagtg aagcgattac cgccaaagtt gaagcggcga
    15301 ttaaagaact caattacgcg ccatcagccc tggcgcgtag cctcaaactc aatcaaacac
    15361 ataccattgg catgttgatc actgccagta ccaatccttt ctattcagaa ctggtgcgtg
    15421 gcgttgaacg cagctgcttc gaacgcggtt atagtctcgt cctttgcaat accgaaggag
    15481 atgaacagcg gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt gatggcttgc
    15541 tgttactgtg caccgaaacg catcaacctt cgcgtgaaat catgcaacgt tatccgacag
    15601 tgcctactgt gatgatggac tgggctccgt tcgatggcga cagcgatctt attcaggata
    15661 actcgttgct gggcggagac ttagcaacgc aatatctgat cgataaaggt catacccgta
    15721 tcgcctgtat taccggtccg ctggataaaa ctccggcgcg cctgcggttg gaaggttatc
    15781 gggcggcgat gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc actggtgatt
    15841 ttgaatttaa cggcgggttt gacgccatgc gccaactgtt atcacatccg ctgcgtcctc
    15901 aggccgtctt taccggaaat gacgctatgg ctgttggcgt ttaccaggcg ttatatcagg
    15961 cagagttaca ggttccgcag gatatcgcgg tgattggcta tgacgatatc gaactggcaa
    16021 gctttatgac gccaccatta accactatcc accaaccgaa agatgaactg ggggagctgg
    16081 cgattgatgt actcatccat cggataaccc agccgaccct tcagcaacaa cgattacaac
    16141 ttactccgat tctgatggaa cgcggttcgg cttagattta cgctgtcttt tgatcaaatt
    16201 attaccatcg gttgttttca gaagcatgaa cattgctgct gaagcaacag taataatgcc
    16261 catcgtgata aacgtatagt ggaattgttc gacagtcgtt gtgccttcca tcccttcata
    16321 aacgcgaagg acggccgcac ttacagcaac gcctaaacta atcgacagtt gctgcgtgac
    16381 cgccagaaca ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa gtgtgatggt
    16441 attcatcgcg gtaaattgcg tcgacatagc catccctaat ataaacaacg gcaagatcag
    16501 catccatatc gccattaccg gtgattgcaa agagaactga gcgatcatta gcccaataat
    16561 caccgtgatc cccactaacg tatggcgata gcccagacga cgtaagactt gggtaaccat
    16621 cgattttgca ataatggaac ctaacgctgt cggtgccatc atacagccgg caataaacgc
    16681 ctgataacca aatcctacct gtaacatcaa tggcataagg aacggtacac aaccggtccc
    16741 cagacgggtt gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt taaataaatc
    16801 taatgaaatt aatgggtttg gcgtgtgtcg cgcatggaga atatagagaa gcagtaaccc
    16861 gatgctggtg acaattaccg tcaaggcaat ccagctggcg acaatctttt ccccgaatag
    16921 ctctattccg cttgagaaga gaacaaggct gaggccaaac agcaaaaagc cagtgatatc
    16981 gaatctgcgt cgtgcggtgg tgaaattggg catatgtttg cgcgcgtaaa gaaggcccgc
    17041 aatacctatg gggatattga ttaaaaatat ccagtgccag gttgcccagg tgaccagcac
    17101 gccgccaaga acggggccta aaattggccc caccagaccc ggcatggcga caaaattcaa
    17161 tactggaaga agttcattac gaggataagc gcgcagtaag gccagccgag caacaggcat
    17221 catcattgcg ccgcctatcc cctgaataac ccggaagaca accagctgtg gtagcgaatt
    17281 agaaagtgcg caggccagag aacccaatgt gaacagactc acggcaaggg taaaaatgcg
    17341 acgcgtaccg aagcgatcgg ctagccatcc gcttaccgga ataagcatcg ccaccgtcag
    17401 cgtataactg atgatggctg attgcatcgc gagaggagaa cgattaaggc tatgagcgat
    17461 tgcgggtaag gcggtattca gaatagtggc atcaagtgcc tgcatgaaga aggccatcgc
    17521 cgcgatccac ggcaaacccg ccatactgcg cttcttttta tcgctcattc aatgtcctat
    17581 tatcgggttg tcacttatca ggtgagcgta gcagcgcctg acaagcttta aatgccgcgt
    17641 cgccatcgct ttggacaatc gcatcgacaa tcgcctggtg cagatccagc tttatcactg
    17701 tgtcgctggt aattgacgtg aagtaagtgt gataaaccga atggaatagc gaggcgaatg
    17761 aggtcaaaaa cggattggcg ctcatttcat agatatgctc atgccaggcc atatcgactt
    17821 cgatccagcg ttcacggcga aagttctctt ttaatgccgc catttcggcc attaacgtat
    17881 tgagatgcgc cttctgttcc gcggtgccaa ccgttgcagc cagtaggcaa gcttgcggct
    17941 ccagacagat acgcataacc agaaagtgat cgatgacctg atgaaagttc tcttctgtca
    18001 tccaccaggt aagcaattcc tgatcaagaa aattccagtt tgattgtggc atgacccggg
    18061 taccaattcg cggtcgcggt aaaaccatcc cttttgccgt taacgttttg accgcttcgc
    18121 gtaccgctgt acgactcact ccaaattgct cgcccagctc aatctcacca ggcaaaatgg
    18181 tgccgggttc atattcacct tttaagatgc gttgcgccag cttctcagcc agaacatacg
    18241 aaaggttttt ctgtgcagct aactgttgtg cgcttaaagg cattactaat cttccttttt
    18301 ctttttattc ctccttagta tgccaccagg aagtgtgatt acggttgcaa aaacggcaaa
    18361 ttgcttgttt tatggcacat taacggggct tttgctgaaa aaatgcgcg
//
LOCUS       DAZKGS010000081        18204 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKGS010000081 DAZKGS010000000
VERSION     DAZKGS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..810)
                     /gene="aroD"
                     /locus_tag="ACHOQY_002855"
     CDS             complement(52..810)
                     /gene="aroD"
                     /locus_tag="ACHOQY_002855"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9678917.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(841..1707)
                     /gene="ydiB"
                     /locus_tag="ACHOQY_002856"
     CDS             complement(841..1707)
                     /gene="ydiB"
                     /locus_tag="ACHOQY_002856"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9678918.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1719..2984)
                     /locus_tag="ACHOQY_002857"
     CDS             complement(1719..2984)
                     /locus_tag="ACHOQY_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9678919.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3211..4425)
                     /locus_tag="ACHOQY_002858"
     CDS             complement(3211..4425)
                     /locus_tag="ACHOQY_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9678920.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(4525..4881)
                     /locus_tag="ACHOQY_002859"
     CDS             complement(4525..4881)
                     /locus_tag="ACHOQY_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9678921.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5017..5124)
                     /gene="rprA"
                     /locus_tag="ACHOQY_002860"
     ncRNA           complement(5017..5124)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOQY_002860"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5310..6422)
                     /gene="ydiK"
                     /locus_tag="ACHOQY_002861"
     CDS             complement(5310..6422)
                     /gene="ydiK"
                     /locus_tag="ACHOQY_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9678922.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6811..9867
                     /locus_tag="ACHOQY_002862"
     CDS             6811..9867
                     /locus_tag="ACHOQY_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9678923.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9864..10274
                     /gene="menI"
                     /locus_tag="ACHOQY_002863"
     CDS             9864..10274
                     /gene="menI"
                     /locus_tag="ACHOQY_002863"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9678924.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10374..10562
                     /locus_tag="ACHOQY_002864"
     CDS             10374..10562
                     /locus_tag="ACHOQY_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9678925.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11110..11478
                     /gene="sufA"
                     /locus_tag="ACHOQY_002865"
     CDS             11110..11478
                     /gene="sufA"
                     /locus_tag="ACHOQY_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9678926.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            11487..12974
                     /gene="sufB"
                     /locus_tag="ACHOQY_002866"
     CDS             11487..12974
                     /gene="sufB"
                     /locus_tag="ACHOQY_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9678927.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12984..13730
                     /gene="sufC"
                     /locus_tag="ACHOQY_002867"
     CDS             12984..13730
                     /gene="sufC"
                     /locus_tag="ACHOQY_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9678928.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13705..14976
                     /gene="sufD"
                     /locus_tag="ACHOQY_002868"
     CDS             13705..14976
                     /gene="sufD"
                     /locus_tag="ACHOQY_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9678929.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14973..16193
                     /gene="sufS"
                     /locus_tag="ACHOQY_002869"
     CDS             14973..16193
                     /gene="sufS"
                     /locus_tag="ACHOQY_002869"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9678930.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16206..16622
                     /gene="sufE"
                     /locus_tag="ACHOQY_002870"
     CDS             16206..16622
                     /gene="sufE"
                     /locus_tag="ACHOQY_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9678931.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16771..17775
                     /gene="ldtE"
                     /locus_tag="ACHOQY_002871"
     CDS             16771..17775
                     /gene="ldtE"
                     /locus_tag="ACHOQY_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9678932.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17839..18075)
                     /gene="lpp"
                     /locus_tag="ACHOQY_002872"
     CDS             complement(17839..18075)
                     /gene="lpp"
                     /locus_tag="ACHOQY_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9678933.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4760 a         4406 c         4689 g         4349 t
ORIGIN      
        1 atatattttt taagttcggc ggggagggtg ttcccgccga aatattattg cttatgcctg
       61 gtgtaaaata gttaataccg tgcgtaaatc atttaccgag atttgccctg gtgcagaggc
      121 tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca gacgagaaat
      181 tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact gctcctgcat
      241 ctccagggtc gcggtaagca acgtcagcac atcgctggta ctttgcggca tcagcgcaat
      301 cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga tttcttcggc
      361 ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat gcgcgtgggc
      421 gtaggcgacg gtttctttaa cctgatcatc gccggtaaat aactccagat cgatcatatc
      481 aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg tggaaatcgc
      541 ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt ctggcatggt
      601 ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt cggcaaagtg
      661 gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag cttcggattt
      721 cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc ccgtaccaat
      781 gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc ggtcagcctg
      841 tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt tgccagtcca
      901 taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa tcgttttgca
      961 accagcttgt tgcgcctgct gcaataactt cgtcatatgc gggttataca cgcattcagt
     1021 gaccagcagt cccggatgta acagactgat atcattaact aatgattcat tctcaagggg
     1081 tttcataccc acttttgtgc cattggttaa aatgtcggcg gaagccaggg cttcagcaaa
     1141 ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt cgttaacccg
     1201 ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga gtttaatttc
     1261 ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa
     1321 cagcaccatt gttttgcctt tgatatcaaa accgctctct ttaatggcgc gaatatggcc
     1381 cgtgccgtcg gtgttatagc cgcgcaggta gccatcatca ttaacgatgg tgttgatggc
     1441 acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca gttgtttgtt
     1501 cggcattgat ataccagttc cgcgcatttt gagggctttt aatccttcaa ttgctccggg
     1561 aaaactatcg ttatccactt caaaggccat ataggtaaat ggcaatcccg ctttttctaa
     1621 ggctttattc tgcatttcag gcgataaact gtggcggata ggataggcca tcagcccaat
     1681 caattcgtat tttgcggtaa catccataat taactccttt aacctctatg cttaattgtg
     1741 tttaaccggg tgctaaattt acgctcgcca aaccgcacat cattctcagg aataataaaa
     1801 acgcggtaat agcggataaa aacaataatt gcggtaatca gggccagcag cgcgaaagta
     1861 aaatcgagaa caatgatata ttgaaggccg atgttcgaca gataaccggt aatcagcgga
     1921 ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt ggctttgctt
     1981 tttgggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc agctgctgag
     2041 aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag gtagataatg
     2101 gctgctgtta ttgttgccag tgcagagtta aatacattag cccagatggg ccggaccatt
     2161 tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact gtaataagag
     2221 atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata tttgggcatc
     2281 cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac cgatgaaaca
     2341 ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc tacgctggcg
     2401 tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag tacaaacaga
     2461 atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat atagctcacc
     2521 agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat gaccgccgaa
     2581 ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc cagcgctgag
     2641 ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg gcaagcggga
     2701 ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc ccgacgacca
     2761 aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa accaataccg
     2821 gaaattaagt aggcaatgcc cgcgttgtca gtggaaaact tttccgccag agatgacatg
     2881 ttttgggcaa gcgtaataac actaatgccg tgcaggaagt agctgaagta aatacaaaga
     2941 acagccagga taaatggcgt gctgaaagcc ttattttgag acattttcac agctccttgc
     3001 tgaaagataa tttcgttacc gtacgtattg ttcttcgacg gtttacataa ttactatact
     3061 gaagatgatt ttccctgggt acatggcata aagtttatgt ttccaggaat attgcatgtc
     3121 atagtttgtt tttgatcgcg attttcctta gttagcgcag aaagagttat ttatccgtaa
     3181 gatcttgcat gacctgaaaa gaatattgca ttacccaccc ggagcgacat tttcctttag
     3241 cgagtgatgc cgcgtttttt tgcggctgcg taagccgata aacagtgcca gtaaaaaacc
     3301 gatggcagcg atggcggtat cgaaccacat aatatcggca atacttcttt gggacagatg
     3361 agccgtaatc aacggaatag taaaggtcgc aatactgccc gcactgtaat agatccctgt
     3421 agctttacct ttagcgtaag ggaaacgttc agccattaac gtcaggccaa tttgcacaac
     3481 acctccagca gaggtaaaac caatgacaaa agcaaatatt atcaccacat aaaatgtggg
     3541 atgcaggcag acggtaaaca gagcaataaa tgagataaag gtgtacagca tcagtaatgt
     3601 tgttgggcga acggtattac gaatgagtgg agcggtaata aatacacaaa gcagcgaatc
     3661 cacggtgtag atactgagta gtttaatcga catagtgtat gacatgcctg caacaaattg
     3721 tccgtactgt gccagccact ggctaaccag ataaaacgtt gccattgaga tatagccata
     3781 taaggtataa ctggctaaat cgataattga acagcgatgt tccgtagagc tggtggtttt
     3841 ctttatgaca ggtaagcgat gacccggatg gggtgggaac gtacaacggt ataaaaacag
     3901 agcgttaata aacataatgc ctgcagcaat cataaaggac caaccgaacc acagttcagc
     3961 ccacactaac aggctaatga ttagcggtaa taaaaattgt ccgctggaaa caaatgcttt
     4021 aattaaaata ttggctgtcc caggtgagcg tggaaaagct tccatcaaac tgggataagt
     4081 gcctgcatcg agaaaactgt ttgccattcc tgccagaaag ccaaaaacat aagcgataat
     4141 gatgttattg gtgtgcagga tgccaaaaaa gaaggccata tagcagcaca tcccgagcat
     4201 gataaaaggg cggcgaccaa agcgatcgga taataatcct gcaaaaagca agacacttaa
     4261 tcgaccaatg cccagcgatg agataactat cgagacaccc gcggcattag tctgccaaag
     4321 tgtctccagc gaggccatat tcaggctcat caaaatgacg cccataccat gcactaggta
     4381 attaaaatac aacccaagtg cggtagggta atagggattt ttcataggtt ctacttagca
     4441 aaggtgcttt aaaaagtgta gcgatgttgt gtcagtatcc gttcattatt gttgtaacaa
     4501 cgacaattcc tttttgtgga gaaattaaca aagccgctcc agatcgtgtg catataattc
     4561 agcggctacc gattgataga tcttccagtc aataaaattg ccatcagggt agacttgttg
     4621 aaacgcggtt aagtcgggga aaaaacgcat ggtgttgttg ccgatgcggt tatttatttt
     4681 ctctatgata gcatgaagat gtttttttcg ttgctgacgc atagccaaca gttgctcgac
     4741 tacacgatca ggaatggcgc atttgccatt ttcccactga cgccagcttt cactgtcacc
     4801 ggtctgggca atccaggtcg cgcattcatc aatagtcatg gcaaaaatat ggcgaagtgc
     4861 ctggagttca taagcgttca tcgtgtaatg gctgcatgaa ataaacaata aaatataaat
     4921 aacattatca gcctgctgac ggcttgaaga gagtcacagt atcttgtgca acattattgg
     4981 aaagagtgag gggcgaggta gcgaagcgga aaaatgttaa aaaaaagccc atcgtgggag
     5041 atgggcaaag actacacaca gcaattcgtt gtttcactca ggggatttcc atgcttataa
     5101 atcaatatgt tgatttataa ccgtaagcta atagtaggca tacggacttt ttgcgtcgat
     5161 cagattcgtc tcattagttt aaaaactgta aagaatttgc gacaggtact accagctgaa
     5221 tggcggtaag caggaaaata tggggaagag caagcaaata tcaattagcg ggggttaaca
     5281 atcaggcggc agagccgcct gatgagaaat tacttattcg gtttctcgat ttcgccaagt
     5341 tcttcgagaa tttcttccgg ttggtccgtc ggcggcggga cttcttccac ccacgcggca
     5401 aacagacgcc aggaaacggc taacagaacc ggaccaataa acagaccgat catcccgaaa
     5461 gcaatcaaac caccaataac accagagagg atcaggatca gcggtaaatc ggcacccatg
     5521 cgaattaaca ttgggcggat gacgttatcc agcgtgccaa ccacaccgct ccacactaac
     5581 aataccgttc cccaggtggt atcgccagtc cagtagagcc agataatcgc cggaatcagt
     5641 accggcaacg ggccaagctg gacaaggcag gagaggatca ttaacaccgt tagcaacgtt
     5701 gcataaggta cgccggatac ggcgaggccg ataccgccaa gcactgcctg tactaacgcc
     5761 gtcaccacca cacccagcgc caccgcgcgg atagcctgtg ccgccagcag tacggcggca
     5821 tctccgcgaa cgcctgccag acgggttgca aaatggcgaa tgccttgtgc cacctgttca
     5881 ccgcgccagt acagcagggc actgaagaga agcatcagcg cacaatgcac cataaagcgc
     5941 ccgatatgcg ccgcctgccc aacgaaccag gtggtggtgg tgccaatata agggcggact
     6001 ttcgccatga tcgccgtccc ccccatatcc agcaagttgt gccagcctgc atacagcttc
     6061 gcgccaatca ccggaatggt attaagccac gccagatcgg gtaacgtcat gtcgccgctg
     6121 gaaatggctt taattagcgg gccgctgccg tcgacgatac tgttaaccag caaagcaata
     6181 gggatgataa acaccatcac taataacagc gtcatcacca gaacggcgag agagcggcgg
     6241 ccaaacatga tcttttgcaa acgtaacaat accggccagg tggcgataac caccgtaccg
     6301 gcccatgcaa agccgagaat aaagggttga acaatccaca gacatgccac aatcatgatg
     6361 gctaaaaaca gcaccgaaag cagaatttgt gcgacatccc tgggctgacg aacatttacc
     6421 ataactactt ttcacctttt ttgtgcgtca aatcgtcgac gcgacgtgaa cacgcgaaac
     6481 tcacctgcat aatgatgagc aatttcagcg gtttttaaca ggccgattct gcccttaatt
     6541 ctgatgggcg tgttagaaaa aaatgtggta caacaactca ggcaacacgc aaacgattta
     6601 ctcgtcgtat ttcaagttgc atgtgctgcg gctacgttcg ttcaccccag tcacttacta
     6661 ttgtaagctc ctggagttca ctcgcttgcc gccttcctgc aactcgaaat ccatagagta
     6721 aataaacatc acctttcagt gcgctattgc agccgcctga aagaatagtg tatcgaccta
     6781 catcacagac cgcaggaaag ggtcaatata atgattccac agatttccca ggcacccggc
     6841 gtcgttcaac tggtgcttaa ttttttgcaa gagctggagc aacaaggttt taccggcgat
     6901 acggcgacaa gttatgccga tcgtctgaca atgtcgaccg acaacagtat ttaccaactt
     6961 ctccccgatg cggtggtatt tccgcgttca accgcagatg tggcgctgat cgcccgtctt
     7021 gccgcgcagg aacgctattc atcgctgatc tttacccccc gtggcggcgg caccggcact
     7081 aacggtcagg cgctcaacca ggggattatt gttgatatgt cccgccatat gaaccgcatc
     7141 atcgaaatta accctgaaga gggctgggtg cgcgttgagg ccggggtgat aaaagatcaa
     7201 ctcaatcagt acctgaaacc gttcggctac ttttttgcgc cggaactttc gaccagcaac
     7261 cgggcaacgc tcggtgggat gatcaatacc gatgcttctg gtcagggatc gctggtctat
     7321 ggcaaaacgt cggatcacgt acttggcgta cgtgcggtgt tgttgggggg cgatattctc
     7381 gatacgcaac ctttacccgt cgaactggcg gaaacgctgg gtaaatccaa taccacaatc
     7441 gggcgaattt ataacacggt ttatcaacgt tgccgtcagc aacgccagtt aatcatcgac
     7501 aacttcccca aacttaaccg ctttcttacc ggttacgatc tgcgtcatgt ctttaacgat
     7561 gagatgaccg agttcgacct gacgcgcatt ctgacgggtt cagaagggac gctggccttt
     7621 attaccgaag cgcggctgga tattacgccc ttgcctaaag tgcgccgtct ggtgaacgtc
     7681 aaatatgact cttttgactc cgcgctgcgt aacgcgccgt ttatggttga ggcgcgggcg
     7741 ctttcggtag agacggtgga ctcaaaagtg ctgaatctgg cgcgggaaga tattgtctgg
     7801 cattccgtca gcgagttgat taccgatgtg cctgacaaag agatgctcgg gctgaacatt
     7861 gtggagtttg ctggtgatga tgaggcgctg attgacgagc gagttaatgc tctctgtgtg
     7921 cggcttgatg agctgattgc cagccaacag gcaggtgtga ttggctggca ggtgtgccgc
     7981 gagctggcgg gtgttgaacg tatctatgcg atgcgtaaaa aagccgttgg tctgcttggc
     8041 aatgccaaag gtgccgctaa gccaattccg tttgctgagg atacctgcgt accgccggaa
     8101 catctggccg attatattgc tgaatttcgc gcgctgctcg acagccacgg cttaagctac
     8161 ggtatgttcg gtcacgtcga cgcaggtgtc ttgcacgtcc gtccagcgct ggatatgtgc
     8221 gatcctcaac aagagatttt gatgaagcaa atctctgatg acgtagtggc gctgactgcg
     8281 aaatacggtg gtttgttgtg gggcgagcac ggcaaaggtt ttcgcgctga atacagcccg
     8341 gcgtttttcg gtgaggaact ttttgcagaa ctgcgcaaag tgaaagcggc atttgacccg
     8401 cataaccgac tcaacccagg gaagatttgc ccgccagaag gtctcgatgc gccgatgatg
     8461 aaagtggacg cggtgaagcg cggtacattc gatcggcaga tccccattgc ggtacgccag
     8521 cagtggcgcg gtgcgatgga gtgtaacggc aacggtttat gcttcaactt tgatgcccgt
     8581 agtccgatgt gtccgtcgat gaagatcacc cagaaccgga ttcattcacc gaaagggcgc
     8641 gcaacgctgg tgcgtgaatg gctgcgtttg ttggcggatc gcggcgttga tccactcaaa
     8701 ctggaacaag aactgcctga atccggcgtc agtttgcgga cgttaattgc ccgcacgcgc
     8761 aatagctggc atgcgaataa aggcgaatat gacttctcac acgaagtcaa agaggcgatg
     8821 tcgggctgtc tggcctgtaa agcgtgttcg acccagtgcc ccatcaaaat tgatgtgccg
     8881 gagtttcgct ctcgttttct gcagctctat cacacccgtt atttacgccc gctgcgcgac
     8941 cacctcgtcg ctacggtcga gagctacgcg ccgctgatgg cacgcgcgcc gaaaaccttt
     9001 aacttcttca ttaaccagcc gctggtgcgc aaactctcgg aaaaacatat cggcatggtt
     9061 gatttgccgc tgctgtcggt cccctcgcta caacaacaaa tggtggggca tcgctcggca
     9121 aacatgacgc tggaacagct tgaatctctc aatgcagagc agaaagcgcg cacagtgttg
     9181 gtggtgcagg acccctttac cagctattac gatgcgcaag tggtggcgga ttttgtccgt
     9241 ctggtcgaaa aattaggttt ccagcctgtg ttactgccat tttcgccaaa tggcaaagcc
     9301 cagcatatta aaggttttct taatcgtttt gcgaagacgg cgaaaaagac ggcggatttc
     9361 ctcaaccgta tggcgaagct gggtatgcca atggtgggcg tcgatccggc gctggtactt
     9421 tgttatcgcg atgaatataa actggcgctg ggcgaggaac gtggcgagtt taacgtctta
     9481 ctggcgaatg aatggctggc aagcgcactt gagtcacagc cagtggctac agtcagcggt
     9541 gaatcatggt atttctttgg tcactgtacc gaagttaccg ccttgccggg tgcgccagca
     9601 caatgggccg cgatatttgc ccgttttggc gcgaaactgg aaaatgtcag cgtgggttgc
     9661 tgcggcatgg cagggactta cggacatgaa gcgaaaaacc ataaaaattc gctcgggatc
     9721 tatgagttat cctggcatca ggctatgcag cgactgccgc gtaaccgctg tctggcgacc
     9781 ggatattcct gccgtagcca ggtaaaacgg gttgaaggca cgggggtacg ccatcctgtg
     9841 caggctttac tggagattat taaatgatat ggaaacggaa aatcaccctg gaagcactga
     9901 atgctatggg tgaaggaaac atggtggggt tcctggatat tcgctttgaa catattggtg
     9961 atgacaccct tgaagcgaca atgccagtag actcgcggac aaagcagcct ttcgggttgc
    10021 tgcatggagg agcatccgtg gtactggccg aaagtatcgg ttccgttgcc ggttatttat
    10081 gtaccgaagg tgagcaaaaa gtggttggtc tggaaatcaa tgctaaccac gtccgctcgg
    10141 cacgagaagg gcgggtgcgc ggggtatgca aaccgttgca tctcggttcg cgtcaccagg
    10201 tctggcagat tgaaatcttc gatgagaaag ggcgtttgtg ctgttcgtca cgattgacga
    10261 ccgccatttt gtgacgttgt caccagaaaa gtgtgacgca tattgccttt ggtcattatc
    10321 tgcacagtaa acggtggtat actgtgcagg tcttgtctaa aagcgaggat gctatgtcta
    10381 ctcaacttga tcccacccag ctggccattg aatttttacg ccgtgaccag agcaatcttt
    10441 cacctgccca atatctcaaa cgtctgaaac aactagaact tgaatttgct gacctgctta
    10501 ctctctcttc tgctgaatta aaagaagaaa tctactttgc ctggcgtctg ggcgtgcatt
    10561 gatcctgact taatgccgca agttcgccaa atcgacctgg attaacctcc gtccaggttc
    10621 gatgaccttc ttaatactgc ttatctaccg cctgatattg ttcaaaaata atactaatcg
    10681 cagtttgtgt taaaacggcg ggttagcttt atgagattat tcttatcgcc ccttcaagag
    10741 ctaagccact gagagtgccg gagataagcg ccggatgggg tagaaaccct taagcctgtg
    10801 ttgcacagac ttaagggttt tcttatttct gaacgctgca cttttttacc tatctcttaa
    10861 atagtcattt tcaatacaac tttttctttt tcatcacagc cttaatgctc cttagataat
    10921 aaccattatc taacaatgag atacctaatt cttagcgtgc ctgttaaccc acacatcagg
    10981 gtctatgctt attaaaagca ggcaaaaagc atgttttacg gtaaagcccc tgcgtttgct
    11041 gggttgtact gataatcatt atcactaaca tgctgttata cgctgaaagc gatgaagtga
    11101 ggtaaatcga tggacatgca ttcaggaacc tttaacccac aagatttcgc ctggcaaggc
    11161 ttaacgctga cacccgcagc ggcgatacac atccgtgagc tggtggcaaa gcagccgggt
    11221 atggtcggcg tgcgcttagg cgtgaagcaa acgggctgcg cgggctttgg ctatgtgctc
    11281 gacagtgtta gcgagccgga caaagacgat ctgctgtttg aacacgacgg cgcgaagctg
    11341 tttgtcccgc tgcaagcgat gccgtttatt gatggcacgg aagtcgattt cgttcgtgaa
    11401 ggacttaatc agatattcaa atttcacaac cctaaagccc agaatgaatg tggctgtggc
    11461 gaaagctttg gggtataggc ggtactatgt ctcgtaatac tgaagcaact gacgatgtca
    11521 aaacctggac cggcggcccg ctgaattata aagaaggatt cttcacccag ttagccaccg
    11581 atgagctggc aaaggggata aacgaagagg tggtgcgcgc aatttcggcg aagcgtaatg
    11641 agccggagtg gatgctgaag tttcgtctaa acgcctatcg cgcatggctg gagatggaag
    11701 aaccgcactg gttgaaagcg cactacgaca agctgaatta tcaggattac agctactact
    11761 cagcaccatc gtgcggtaat tgtgacgaca cttgcgcgtc tgaacctggc gcggtgcagc
    11821 aaactggcgc gaacgccttt ttaagtaaag aggtggaggc ggcgtttgag cagttgggcg
    11881 ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat tttcgactca gtttcggttg
    11941 ccactactta tcgcgaaaaa ctggcggagc agggaattat tttctgttcc tttggtgagg
    12001 cgatccacga tcacccggaa ctggtgcgta aatatctcgg caccgtggtg ccggggaatg
    12061 acaacttctt tgccgcgctt aatgcggcgg tagcctctga cggtacgttt atttatgtgc
    12121 ctaaaggcgt gcgctgcccg atggaacttt ccacctattt tcgcattaac gcggaaaaaa
    12181 ccgggcagtt tgagcgcacc attctggtgg ccgacgaaga cagctacgtc agctacattg
    12241 aaggctgttc cgctccggtg cgtgacagct atcagttaca cgcggcagtg gtggaagtca
    12301 tcatccataa aaacgccgag gtgaaatatt ccacggtaca aaactggttc cctggcgata
    12361 acaacaccgg cggtattctc aacttcgtca ccaagcgtgc tttgtgcgaa ggcgaaaaca
    12421 gcaaaatgtc atggacgcaa tcagaaaccg ggtccgcgat tacgtggaaa tatcccagct
    12481 gcattttgcg cggcgataac tccattggtg agttttactc agtggcgctg accagcggtc
    12541 atcagcaagc ggataccggc accaagatga tccacatcgg taaaaacacc aaatcgacca
    12601 ttatctcgaa agggatctct gccggacata gtcagaacag ttatcgcggc ttagtgaaaa
    12661 tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg cgactcaatg ctgattggcg
    12721 ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg caataacagc gcacaactgg
    12781 agcacgaggc aacgacatca cgtattggtg aagatcaact gttttactgc ctgcaacgcg
    12841 ggatcagcga agaagacgcc atctcgatga ttgttaacgg tttctgcaaa gacgtgttct
    12901 cagaactgcc gctggaattt gccgttgaag cacaaaaact cctcgccatc agtcttgaac
    12961 acagcgtcgg ataaggaata aacatgttaa gtattaaaga tttacacgtc agcgtggaag
    13021 ataaagctat cctgcgtgga ttaagcctcg ccgttcgtcc cggcgaagtt cacgccatta
    13081 tggggccaaa cggttcgggc aaaagtacct tatcggcaac gcttgccggg cgcgaagatt
    13141 atgaagtgac gggcggcacg gttgagttca aaggcaaaga tttgcttgca ctgtcgccgg
    13201 aagatcgtgc aggcgaaggc atctttatgg ccttccagta tccggtggag attccaggcg
    13261 tcagtaacca gtttttcctg caaacggcac ttaatgcagt gcgcagctat cgcggccagg
    13321 aaacgctcga ccgctttgat tttcaggatt tgatggaaga gaaaatcgct ctcctgaaga
    13381 tgccggaaga tttattaacc cgttcggtaa acgttggttt ttccggcggc gagaaaaagc
    13441 gcaacgatat tttgcaaatg gcggtgctgg aaccggagtt atgcattctt gatgagtcgg
    13501 actccgggct ggatattgac gcattaaaag tggtcgccga tggcgtgaac tcgctgcgtg
    13561 atggcaagcg ctcattcatc attgttacgc actaccaacg cattctcgac tacatcaagc
    13621 ctgattacgt ccatgtgcta tatcggggac gaattgtgaa atccggcgat ttcacgttgg
    13681 tcaaacaact ggaggagcag ggttatggct ggcttaccga acagcagtaa cgcgctgcaa
    13741 cagtggcatc acttgtttga agctgaaggg acaaaacgct caccgcaagc acagcagcat
    13801 ttacaacaat tgctgcgtac cggactgccg acacgtaaac atgaaaactg gaaatatacg
    13861 ccgctggaag ggctgaccaa tagccagttt gtcagcattg cgggagagat atccccacag
    13921 cagcgagatg ccttagcgtt aacgttagac gctgtgcggc tggtatttgt cgatggacgt
    13981 tacgtgtcag cactgagcga tgcgactgaa ggcagcgggt atgaagtgag cattaacgac
    14041 gaccgtcagg gggtacccga cgctattcag gcggaagtgt ttctgcattt gacggaaagc
    14101 ctggcacaaa gcgtgacgca tatcgccgtg aagcgcggtc aacggccggc aaagccattg
    14161 ctgttaatgc atattaccca gggcgtggca ggtgaagagg tcaacactgc ccattaccga
    14221 catcatctgg atctggcgga aggtgccgaa gcaacggtga tcgaacattt tgtcagcctt
    14281 aatgatgctc gtcacttcac cggcgcacgg ttcactatca atgtcgcagc gaacgcccac
    14341 ttgcagcata tcaagctggc gtttgaaaac ccggtcagtc accactttgc ccataacgat
    14401 ttgttgctgg ctgacgatgc caccgcattt agccatagtt tcctgctggg tggcgcagtg
    14461 ttacgacaca acaccagtac gcaactcaat ggcgaaaaca gcacgctgcg gatcaatagc
    14521 ctggcgatgc cggtgaaaaa cgaggtgtgt gatacccgta cctggctgga acacaataaa
    14581 ggtttttgta acagccgaca gttgcacaaa actatcgtca gcgacaaagg ccgcgcggta
    14641 tttaacggtt tgatcaacgt cgcgcagcac gccatcaaaa cggatggtca gatgaccaac
    14701 aataatctgc tgatgggcaa actggcagaa gtggatacga aaccgcagct ggaaatctat
    14761 gcagatgatg tgaaatgcag ccacggcgcg acggtggggc gtattgatga tgaacagatg
    14821 ttctatctgc gctcgcgcgg gatcaatcag caggatgccc agcagatgat catttacgcc
    14881 tttgctgccg aactgacgga agcactgcgt gatgaggggc ttaaacagca ggtgctggcc
    14941 cgaatcggtc aacggctgcc aggaggtgca agatgacttt ttccgtcgac aaagtgcggg
    15001 ccgactttcc ggtgctttct cgtgaggtaa acggtttgcc gctggcttat ctcgacagcg
    15061 ccgccagtgc gcagaaaccg agccaggtga ttgacgccga ggccgagttt tatcgtcatg
    15121 gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc ccaagcgacc gagaaaatgg
    15181 agaacgtgcg caagcgggca tcgctgttta ttaatgcccg ttcggcggaa gagctggtgt
    15241 tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa tagctggggt aacagcaatg
    15301 tgcgggcggg cgataacatc atcatcagtc agatggagca ccacgctaac attgttccct
    15361 ggcagatgct ttgcgcacgc gttggcgcag agctgcgtgt gatcccgctc aatcccgacg
    15421 gtacgttgca actggagacg ctgcctacgc tgtttgatga gaaaactcgc ctgctggcaa
    15481 ttactcatgt ctccaacgtg cttggcacag aaaatccact ggcggaaatg atcacgcttg
    15541 cgcaccagca tggcgcaaaa gtgctggtgg atggcgctca ggcggtgatg catcatccgg
    15601 tggatgttca ggcgctggat tgcgactttt acgtgttctc cgggcataaa ctgtatggcc
    15661 ccaccggaat tggcattctt tatgtgaaag aagccttgtt gcaggagatg ccgccgtggg
    15721 aagggggcgg ttctatgatc gccaccgtca gcctgagtga aggcacaacc tggaccaaag
    15781 caccttggcg gtttgaagcc ggtacaccca ataccggggg catcattggt cttggcgcgg
    15841 cgctggagta tgtttcggcg ctggggctta ataacatagc cgagtatgaa cagaatctga
    15901 tgcactacgc gctatcacag ctggaatctg taccggatct cactctctat ggtccgcaga
    15961 acaggcttgg cgttattgct tttaatcttg gtaaacacca cgcctatgat gttggcagtt
    16021 ttctcgataa ctacggcatt gctgtgcgta ccggacatca ctgcgctatg ccattaatgg
    16081 cctattacaa cgtccctgct atgtgtcggg cgtcgctggc catgtataac acccatgaag
    16141 aagtggatcg tctggtgacc ggactgcaac gtattcaccg tctgctggga taacagggag
    16201 gcactatggc tttattgccg gataaagaaa aattgctgcg taatttttta cgctgcgcca
    16261 attgggaaga gaaatatctc tacattattg agctgggcca gcgtctgcca gaattacgcg
    16321 acgaagacag aagcccacaa aatagcattc agggctgcca gagtcaggtg tggattgtca
    16381 tgcgccagaa tgcgcaggga attattgaat tgcagggcga cagcgatgcg gcgattgtga
    16441 aagggcttat tgcggtcgtc tttattctct acgatcagat gacgccgcag gatattgtca
    16501 atttcgatgt gcgtccgtgg tttgaaaaaa tggcgctcac ccaacatctc accccatctc
    16561 gttcacaagg tctggaagcg atgattcgcg caattcgcgc caaagccgct gcacttagct
    16621 aaactaaagg gacagctttc atccggttgg ctcacattgc cggatggtca atgtttacac
    16681 tgacctgcac aggagtgttt cggtttcagg tgaacataag cgccatccga cgtttcagcg
    16741 tgagtctccg gcaaatacag gaggtttaca atgaaacgcg cgtctttgct tacactcacg
    16801 cttatcggcg cttttagcgc catccaggct gcctgggcgg ttgattatcc gctaccacca
    16861 accggaagcc gactggttgg gcaaaatcaa acgtatacgg tgcaggaagg ggataaaaac
    16921 cttcaggcca tcgcccgacg ttttgatact gcggcaatgt tgatccttga agccaataac
    16981 actatcgccc cggtgccaaa acccggtacg acgataacta ttccttcgca actgttatta
    17041 cctgatgcgc cgcgtcaggg gattatcgtt aaccttgcag agctgcgcct ttattattat
    17101 ccgccgggag aaaatatcgt gcaggtttat ccgataggta ttggcttgca ggggctggaa
    17161 acgccggtga tggaaacgcg tgtggggcag aaaatcccta acccaacctg gacgcctact
    17221 gcaggcattc gtcaacgttc gctggagcgt ggtattaaat taccgccagt cgttcctgcc
    17281 ggaccaaata acccgctagg acgttacgca ctgcgcctcg cgcatggtaa tggcgaatac
    17341 ctcattcatg gcaccagtgc gccggacagc gtcggtttgc gcgtcagttc agggtgtatt
    17401 cgcatgaatg caccggatat taaagccttg ttctccagcg tgcggacggg aacgccggtg
    17461 aaagtgatca acgaacctgt gaaatattcc gtggagccta acgggatgcg ttatgttgaa
    17521 gtacatcgac cactatcggc agaagaacag cagaacgttc agacaatgcc atacacactg
    17581 ccagcaggct ttacgcaatt taaagacaat aaggctgtag atcagaagtt agtcgataaa
    17641 gcgttgtatc gtcgggcagg gtatccggtt tcggtgagca gtggagcaac tcccgcagcc
    17701 agcaatgcgc cttcagtaga gtcagcgcag aatggtgaac cagagcaagg gaatatgtta
    17761 cgcgcgacgc agtagcggta aacggcagac aaaaaaaatg gcgcacaatg tgcgccattt
    17821 ttcacttcac aggtactatt acttgcggta tttagtagcc atgttgtcca gacgctggtt
    17881 agcacgagct gcgtcatctt tagcagcctg aacgtcggaa cgcattgcgt tcacgtcgtt
    17941 gctcagctgg tcaactttag cgttcagagt ctgaacgtca gaagacagct gatcgatttt
    18001 agcgttgctg gagcaacctg ccagcagagt agaacccagg attaccgcgc ccagtaccag
    18061 tttagtagct ttcattatta ataccctcta gattgagtta atctccatgt agcgttacaa
    18121 gtattacaca aagtttttta tgttgagaat atttttttga tgggaaggca cttatttttg
    18181 atcgttcgct caaagaagca tcga
//
LOCUS       DAZKGS010000082        18113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKGS010000082 DAZKGS010000000
VERSION     DAZKGS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..806
                     /locus_tag="ACHOQY_002873"
     CDS             78..806
                     /locus_tag="ACHOQY_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9678934.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            806..5656
                     /gene="ehaG"
                     /locus_tag="ACHOQY_002874"
     CDS             806..5656
                     /gene="ehaG"
                     /locus_tag="ACHOQY_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP9678935.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6025..7680
                     /gene="lldP"
                     /locus_tag="ACHOQY_002875"
     CDS             6025..7680
                     /gene="lldP"
                     /locus_tag="ACHOQY_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9678936.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7680..8456
                     /gene="lldR"
                     /locus_tag="ACHOQY_002876"
     CDS             7680..8456
                     /gene="lldR"
                     /locus_tag="ACHOQY_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9678937.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8453..9643
                     /gene="lldD"
                     /locus_tag="ACHOQY_002877"
     CDS             8453..9643
                     /gene="lldD"
                     /locus_tag="ACHOQY_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9678938.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9829..10302
                     /gene="trmL"
                     /locus_tag="ACHOQY_002878"
     CDS             9829..10302
                     /gene="trmL"
                     /locus_tag="ACHOQY_002878"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9678939.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10355..11176)
                     /gene="cysE"
                     /locus_tag="ACHOQY_002879"
     CDS             complement(10355..11176)
                     /gene="cysE"
                     /locus_tag="ACHOQY_002879"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9678940.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11256..12275)
                     /gene="gpsA"
                     /locus_tag="ACHOQY_002880"
     CDS             complement(11256..12275)
                     /gene="gpsA"
                     /locus_tag="ACHOQY_002880"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9678941.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12275..12742)
                     /gene="secB"
                     /locus_tag="ACHOQY_002881"
     CDS             complement(12275..12742)
                     /gene="secB"
                     /locus_tag="ACHOQY_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9678942.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12805..13056)
                     /gene="grxC"
                     /locus_tag="ACHOQY_002882"
     CDS             complement(12805..13056)
                     /gene="grxC"
                     /locus_tag="ACHOQY_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9678943.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13198..13629)
                     /locus_tag="ACHOQY_002883"
     CDS             complement(13198..13629)
                     /locus_tag="ACHOQY_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9678944.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13874..15418
                     /gene="gpmM"
                     /locus_tag="ACHOQY_002884"
                     /gene_synonym="pgmI"
     CDS             13874..15418
                     /gene="gpmM"
                     /locus_tag="ACHOQY_002884"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9678945.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15452..16711
                     /gene="envC"
                     /locus_tag="ACHOQY_002885"
     CDS             15452..16711
                     /gene="envC"
                     /locus_tag="ACHOQY_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9678946.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16715..17674
                     /locus_tag="ACHOQY_002886"
     CDS             16715..17674
                     /locus_tag="ACHOQY_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9678947.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17661..>18113)
                     /gene="waaH"
                     /locus_tag="ACHOQY_002887"
     CDS             complement(17661..>18113)
                     /gene="waaH"
                     /locus_tag="ACHOQY_002887"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP9678948.1"
                     /translation="TTEFMFNALRARYTEQSLYKYYLHNTSVSRLHRQGNKNLNYQRH
                     YIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFT
                     SGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         4538 a         4683 c         4730 g         4162 t
ORIGIN      
        1 gcaacataga tcaccaataa tacaatggac aactctaccg gcattgatcg ctggtcggag
       61 gaaataagga tttttacatg aacttgaaga agattttttt cagtgctgtc actgtttcag
      121 ttctctgcgc attaacggga tgtgactaca ttgaagaagg caagccagaa agtagtttat
      181 tgaaacaaca agaagaacat aacaataaaa ttgatctact tgaaaaacaa caagcacagc
      241 taaagagtca actggaaact attcaaaaac aacaaactgg cataataagc agtacaaaga
      301 ctttaactca cgtaattaaa tccgttaaag atcaacaaaa cacttttatt tttaccgagt
      361 ttaacccagc aaaaacgaag tatttcatcc tgaacaatgg ttcggtggct ttagcgggtc
      421 gagtgttatc cattgacgcc acagagaatg gcagtgttat tcatatttca ctggtcaact
      481 tattaagtac acctatctca aatattggtt ttaatgcgac atggggtggt gaaaaacctg
      541 tcgatgccaa agagtttgcc cggtggcaac aattactttt caacacatca atgaaatcca
      601 cattgaaatt attaccaggt caatggcaag acattaattt gaccctgaag ggtgtatcgc
      661 ccaataacct gggatatctg aagttagcca tcaacatgga aaatattcag ttcgacaatc
      721 ttccttctgc tgaaaatcgg cagaaaagaa gcaaaaaaat aaatgcagtt agtacgctta
      781 taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc
      841 gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg
      901 cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt
      961 ggcgaatgcc gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga
     1021 cggctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc
     1081 cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc
     1141 aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg
     1201 cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct
     1261 cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact cttccaaagc
     1321 ggaaggagca aacgccattg ccctgggaaa tgccactaaa gctactgaga ttatgagtat
     1381 tgctcttggc gacaccgcca atgcgtcaaa agcgtattca atggcgctgg gagcaagtag
     1441 cgtcgcatct gaagaaaacg caattgccct ggggcgtagc agtgtagcta gcggtactga
     1501 cagcctcgca tttggcagac aatcacttgc cagcgcagcg aacgctattg cgataggtgc
     1561 tgagaccgaa gccgctgaaa atgcaactgc tattggcaat aatgcgaagg caaaagggac
     1621 taatagcatg gcaatggggt tcggaagcct tgccgataaa gtcaatacta tcgcattagg
     1681 aaatggcagc caggctctgg cagataatgc aatcgccata ggccagggca acaaagctga
     1741 tggcgtggat gccatcgctc tgggtaatgg tagccagtcg agaggcttaa acaccattgc
     1801 cttaggcaca gccagtaatg caactggtga taagagtctt gcgcttggta gtaatagcag
     1861 tgccaacggt attaactctg tcgcgctggg cgcagattcc attgcggatt tagacaatac
     1921 cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa atggcgcgat
     1981 caagtctgac agttacgatg ccattaatgg ttcacagctt tatgccatta gcgactcggt
     2041 agcaaaaagg cttggaggag gggctgcagt agatgttgat gacggtactg ttacagcacc
     2101 aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg ctgtacttga
     2161 tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac agcgctgctc atggtactag
     2221 tagcccaact gccagcgtaa tcaccgatgt tgcggatggc acgatttcag cctccagtaa
     2281 ggatgcggtt aacggttccc aactgaaagc taccaatgac gatgtcgaag ccaacaccgc
     2341 caatatcgct actaatacca gcaacattgc cacgaatacg gcaaatattg ccaccaatac
     2401 caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg ccctgctctg
     2461 gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca gcaaaatcac
     2521 caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg gctctcagct
     2581 gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata acacttccaa
     2641 tattgccact aacaccacca acatctctaa cctgactgag acggtgacta atcttggtga
     2701 ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg gcaccgagac
     2761 caccagcaaa atcaccaacg ttaaagatgg cgacctgacg actggcagca ccgatgccgt
     2821 taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca ccaatatcgc
     2881 cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg gtgaggatgc
     2941 gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca ataccgccag
     3001 caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg ccattaacgg
     3061 tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca atgcttctgt
     3121 gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa attattataa
     3181 cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg gcgatgctct
     3241 gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg gtgacgcaag
     3301 cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg cagtaaacgg
     3361 ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg gtgccgaagt
     3421 caatgcagac ggcaccatca ctgcgccgac gtacaccatt gctaatgctg attacgataa
     3481 tgtcggtgat gccctgaatg ctatcgatac cactcttgac gacgctctgc tctgggatgc
     3541 ggacgccggt gaaaatggtg catttagcgc cgctcacgga aaagataaaa ctgccagtgt
     3601 aatcactaac gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga ttaacggctc
     3661 acaactctat accaccaata agtacatcgc tgatgcgctg ggtggtgacg cagaagtcaa
     3721 cgctgacggc accatcaccg caccgactta caccattgcg aacgccgagt acaacaacgt
     3781 cggtgacgcc ctggatgcgc ttgatgataa cgccttgctg tgggatgaga ctgccaatgg
     3841 cggtgctgga gcctacaatg ccagccatga cggtaaagcc agcatcatca ctaatgtcgc
     3901 taatggcagt attagtgagg acagtaccga tgcagtgaac ggttctcagt tgaatgcgac
     3961 gaatatgatg attgagcaga acacccaaat tatcaatcag ctcgctggta acaccgacgc
     4021 aacctatatc caagaaaacg gtgcgggtat taactatgtg cgtactaacg acgacggctt
     4081 agcgttcaac gacgccagcg cacagggtgt tggcgctaca gctataggtt ataactctgt
     4141 cgccaaaggc gatagcagcg tagctattgg tcagggcagc tacagcgacg ttgatacggg
     4201 tatcgccctg ggtagcagct ctgtttccag ccgagtgatt gccaaaggct cccgtgacac
     4261 cagcataacg gaaaatggcg ttgttattgg ttacgacacc acggatggcg aactgctcgg
     4321 tgcattgtct atcggtgatg acggtaaata tcgtcaaatc atcaacgtag ccgatggttc
     4381 cgaagcccat gacgccgtta cggttcgtca attgcagaat gcgattggtg cggtcgcaac
     4441 cacgccgact aaatacttcc acgctaattc aacggaagaa gattcactgg cagtgggaac
     4501 tgactcgctg gcaatgggtg cgaaaaccat cgtgaatggc gataaaggta ttggtatcgg
     4561 ttatggtgcc tacgtggacg cgaatgcact taacggcatt gccattggta gcaatgcgca
     4621 agtcattcat gtcaacagta ttgcgatagg taatggttct acgaccactc gtggcgctca
     4681 aaccaattat accgcctaca acatggacgc accgcagaac tctgtcggtg aattctcagt
     4741 cggtagtgcg gatggtcaac gtcagatcac taacgtcgca gcaggttcgg ctgataccga
     4801 tgcggtcaac gtgggtcagt tgaaagtaac ggatgcgcag gtttcccaga atacccagag
     4861 cattactaac ctgaacaatc aggtcactaa tctggatact cgcgttacta atatcgaaaa
     4921 cggtattggc gacattgtca cctccggtag caccaagtac ttcaagacca acaccgatgg
     4981 cgtagatgcc aacgcccagg gtaaagatag cgttgctatt ggttctggtt ccattgctgc
     5041 cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca gaggaagaaa atacaatctc
     5101 tgtaggttct tctactaacc aacgccgtat caccaacgta gctgccggtg ttaatgccac
     5161 cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca ggcggcgtgc gctacgacac
     5221 caaagctgat ggttctatcg actatagcaa tatcaccctc ggtggtggca acggcagtac
     5281 gactcgtatc agcaacgtct ccgctggcgt caacaacaac gacgcggtga actacgcgca
     5341 gttgaagcaa agcgtgcagg aaacgaagca atacaccgat cagcggatgg ttgagatgga
     5401 taacaaactg tctaaaactg aaagcaagtt gagcggtggt atcgcttctg caatggcaat
     5461 gaccggtctg ccgcaggctt atacaccggg tgccagcatg gcttctattg gtggcggtac
     5521 ttacaacggt gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca atggtcgttg
     5581 ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa tactccgccg cactcggtgc
     5641 cggtattcag tggtaatgat ccattaagtt agtgtgacta agggcaaacg ccaaattgcc
     5701 tgatgcgtta cgcgcatcag gcaagataag gcgctccggg tcaacaatct aagggcaatt
     5761 ctctgatgag gattgccctt ttctttacca gacatctccc ccccacaaga attggcccta
     5821 ccaattcttc gcttatctga cctctggttc acaattcccc aattaaaact cacatcaata
     5881 ttgccaatac ataacattta gttaaccatt cattgtcatt atccctacac aacacaattg
     5941 gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc aacagactca ttacacgatg
     6001 tgcgtggact ccaggagacc tgcaatgaat ctctggcaac aaaactacga tcccgccggg
     6061 aatatctggc tttccagtct gatagcatcg cttcccatcc tgtttttctt ctttgcgctg
     6121 attaagctca aactgaaagg atacgtcgcc gcctcatgga cggtagcaat cgcccttgcc
     6181 gtggctttgc tgttctataa aatgccggtc gctaacgcgc tggcctcggt ggtttatggc
     6241 ttcttctatg gtttgtggcc catcgcgtgg atcattattg cagcagtgtt cgtctataag
     6301 atctcggtga aaaccgggca gtttgacatc attcgctcgt ctattctttc gataacccct
     6361 gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgaaggagcc
     6421 gcaggctttg gcgcaccggt agcaattacc gccgcattgc tggtcggcct gggttttaaa
     6481 ccgctgtacg ccgccggact gtgcctgatt gttaacaccg cgccagtggc atttggcgcg
     6541 atgggcattc caatcctggt tgccggacaa gtaacaggca tcgacagttt tgagattggc
     6601 cagatggtgg ggcgtcagct accgtttatg accattatcg tgctgttctg gatcatggcg
     6661 attatggacg gctggcgcgg tatcaaagag acgtggcctg cggtcgtggt tgcgggcggc
     6721 tcgtttgcca tcgctcagta ccttagctcc aacttcattg ggccggagct gccggacatt
     6781 atctcttcgc tggtatcact gctctgcctg acgctgttcc tcaaacgctg gcaaccagtt
     6841 cgcgtattcc gcttcggtga cttgggggcg tcacaggttg atatgacgct ggcccacacc
     6901 ggttacaccg cgggtcaggt gttacgtgcc tggacaccgt tcctgttcct gaccgctacc
     6961 gtaacactgt ggagcatccc gccgtttaaa gccctgttcg catcgggtgg tgcgctgtat
     7021 gagtgggtga tcaacattcc ggtgccgtac ctcgataaac tggttgcccg tatgccacca
     7081 gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt ttgactggtt ctctgccacc
     7141 ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct ggctgaagat gaaaccgtct
     7201 gacgctatca gcaccttcgg cagcacgctg aaagaactgg ctctacccat ctactccatc
     7261 ggcatggtgc tggcgttcgc ctttatctcg aactattccg gactgtcatc aacactggca
     7321 ctggcactgg cgcacaccgg tcatgcattc accttcttct cgccgttcct cggctggctg
     7381 ggggtattcc tgaccgggtc ggatacctca tctaacgccc tgttcgccgc gctgcaagcc
     7441 accgcagcac aacaaattgg cgtctctgat ctgttgctgg ttgccgccaa taccaccggt
     7501 ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta tcgcctgtgc ggcggtaggc
     7561 ctggtgggca aagagtctga tttgttccgc tttactgtca aacacagcct gatcttcacc
     7621 tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct taacgtggat gattccttaa
     7681 tgattgtttt acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg
     7741 atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga gcgccaactg gcgatgcaac
     7801 tcggcgtatc acgtaattca ctacgcgagg cgctagcaaa actggtgagc gaaggcgtgc
     7861 tgctcagtcg acgcggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc
     7921 aaaacatcgt ccagccgcta aaaacgctga tggccgatga tccggattac agtttcgata
     7981 ttctggaagc ccgctacgcc attgaagcca gcaccgcatg gcatgcggca atgcgcgcca
     8041 cacctggcga caaagaaaag attcagcttt gctttgaagc aacgctaagt gaagacccgg
     8101 atatcgcctc acaagcggac gttcgttttc atctggcgat tgccgaagcc tcacataaca
     8161 tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct gcaatcctca gtgaagcata
     8221 gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca actgaccgaa caacatcagg
     8281 ctgtcattga cgccattttt gctggtgatg ccgacggggc gcgtaaagca atgatggcgc
     8341 accttagttt tgttcacacc accatgaaac gattcgatga agatcaggct cgccacgcac
     8401 ggattacccg cctgcccggt gagcataatg agcattcgag ggagaaaaac gcatgattat
     8461 ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt tcctgttcca
     8521 ctatatggat ggtggtgcat attctgaata cacgctgcgc cgcaacgtgg aagatttgtc
     8581 agaagtggca ctgcgccagc gtattctgaa aaacatgtcc gacttaagcc tggaaacgac
     8641 gctgtttaat gagaaattgt cgatgccggt agcactggca ccggtgggtt tgtgtggcat
     8701 gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg gacgcgcatg gcattccgtt
     8761 tactctctcg acggtttccg tttgcccgat tgaagaagtc gcgccagcca tcaagcgccc
     8821 aatgtggttc cagctttatg tactgcgcga tcgcggcttt atgcgtaacg cgctggagcg
     8881 agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg gatatgccga caccgggcgc
     8941 acgctaccgt gatgcgcatt caggtatgag cggcccaaat gcggcaatgc gccgctactt
     9001 gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccacatga
     9061 tttaggtaat atctcagctt atctcggcaa accgaccgga ctggaagatt acatcggctg
     9121 gctggggaat aacttcgatc cgtccatctc atggaaagac cttgagtgga tccgcgattt
     9181 ctgggatggc ccgatggtga tcaaagggat cctcgatccg gaagatgcgc gcgatgcagt
     9241 acgttttggt gctgatggaa ttgtggtttc taaccacggt ggccgccagc tggacggtgt
     9301 actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg gtgaaaggtg atatcgccat
     9361 tctggcggat agcggaattc gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc
     9421 cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg
     9481 tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa gtggcgatga cgctgactgg
     9541 cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg gtaaagagtt
     9601 gcctacggca ctggctccga tggcgaaagg gaatgcggca tagtcgtttg cgccccctca
     9661 ccctaaccct ctccctcagg gagaggggac cgttcggcgc tgtatgtact ccctcactct
     9721 gaaacgacac cgcactcttt ttttctccct cgcccctccg gggtgagggg aaaaggccgc
     9781 actgctcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc
     9841 gtactttacg aaccagaaat tccgccaaat actggcaata tcatccgtct ttgcgctaat
     9901 accggctttc gtctgcatat catcgaaccg atgggatttg cctgggacga taagcgcctg
     9961 cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactttcgc
    10021 gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcactacgaa aggtacgcct
    10081 gctcacagcg ccgtaaacta tcaggatggc gattatctga tgttcggccc ggaaacacgc
    10141 ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg cattccaatg
    10201 gtgccggaca gccgcagcat gaacctgtcc aatgcggtgt cggtagtggt gtatgaagcc
    10261 tggcggcagc tgggatatcc gggagcgttg ttgagagatt agttactgta tgccggatag
    10321 atgattacat cgcatccggc acgatcacag cacatcagat cccatcccca tactcaaatg
    10381 tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg ctgtctggtt
    10441 taccgacaat acgagccgga acaccagcgg cggtagtatg cggcggcacc ggttgcagca
    10501 ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg agaattttcg
    10561 cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg acggtcacca ccagatttac
    10621 ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt accgccgttt
    10681 caccaacgac gatgcccgtg gcgtggtcga gcatgatacc gcgaccaatt tttgctgccg
    10741 ggtgaatatc gacctggaac gtcacagaaa cctggttttg cagaaagatt gccagtgcgc
    10801 gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca tgaaaaccct
    10861 tcaggtataa caacggggtt gagtatttat cgactgccgg gtcgcgggta cgcaccgcct
    10921 gaatatcaca ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct tcttcaacca
    10981 cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc agcatgtagc
    11041 tcagggcact gccaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca
    11101 ttggctcaca gtccgccagc gttctggctt cggctttaat attgttccag acaatttcca
    11161 gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg
    11221 ggccgggtca tagcggtaac aaaggttccc tggggttagt ggctgctgcg ctcgtccttg
    11281 cgtgcacgac ctagtaaagt caatgctgcc tcgcgcgcgt tttttccgca atataatact
    11341 tgataaattt cctcggttat tggcatttca acgccgaagc gatgcgccag ttcgcggact
    11401 tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc gctttgtaca
    11461 tccatgccct gaccgagcat catgccaaaa cggcggttac gcgactggtt gtcggtacag
    11521 gtaagcacca gatcgcccag ccccgccatg cccataaagg tggcaggatc ggcacccagc
    11581 gccgcaccaa gacgcgacat ttcagccagc ccacgggtga tcagcgccgt acgcgcattc
    11641 gcaccaaaac cgataccgtc agacatcccc gcgccaatgg caatgacgtt tttcaccgcg
    11701 ccgccaagct gcacgccaat gaaatccggg ttgctgtaaa cgcggaaact tttgccacag
    11761 tgcagcaatt gttggagatc atcggcaaag gtctggtcgg tagaggccaa cgaaatagcc
    11821 gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac cgccagcgga
    11881 atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca gacgtccggt ttccgcttcc
    11941 agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt aatctggcgc
    12001 agcacttcac caaagacatg gctgggtacg acgacgagaa tattacggct ggctgccagc
    12061 gcagtggcga gatcgctttc aagatggagc gtatcgggaa aaggcacatc ggggagaaac
    12121 gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg gccccagagg
    12181 acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta cgagccggca
    12241 ccgatcacag tcattgaagc attacgttgg ttcatcaggc atcctgatgt tcttcagtac
    12301 cttcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta accggcgcaa
    12361 ggttcagttg cgggaatgta ccgcgggata ccatgctggt gatgcactca cgagcatacg
    12421 ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg ccttcgatac
    12481 ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt tcttcgccca
    12541 gggaggccgt tacggtaaca cgcagtacca cttcgtatac gtcatctgcc agttgggaag
    12601 atgccgtatc cagatcaagt ttaacttctg gttgccaatc tttctggaaa acgtgcggcg
    12661 cgttcggcgc ttcgaaagag atatccttgg tataaatacg ttggatctgg aaagtcattt
    12721 cagtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt aaatactgcg
    12781 caatgtaatg ccaacgttca cacgttattt cagcagggga tccagtccac cacgtgcatc
    12841 caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa tctggggaac
    12901 cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc catcgatcgg
    12961 caactcctga aaactcacgc ccttgctgct cagcagtgct tttgcacgat ggcaatacgg
    13021 gcaggtttct ttggtataga tttcaacatt ggccataatt tagctcctgt ttttttaccc
    13081 tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc cccagaagct tataacaata
    13141 agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac aggtaaatta
    13201 tttgccgcgc accagaggca agttttcgcc agcccagcca gcaacgccct ctttcagcac
    13261 gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca ggctcctggc actgcatgcc
    13321 agaaccgtct accacgataa ccggtttgtc tttgtgcttc tcaagctcac caacattgtt
    13381 ggctttgatt tcactcggca acagattgat agaacctgcg atatggcctt tacggaagtc
    13441 atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac gcgtagcttc
    13501 accacgagta atcaccttca ctttcgaggt caggctctta aacgtagtca caagaaccgc
    13561 caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa attgcataat
    13621 ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg aaaggagtat
    13681 acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt tgcggggcac
    13741 gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat attttgatct
    13801 tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga ctcttgagta
    13861 tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc tggatggcta
    13921 tggctatcgt gaagaacagc aggataacgc catttttagt gctaaaaccc cggtaatgga
    13981 tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg aagtcggtct
    14041 gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg ccggccgcat
    14101 cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt tctttgctaa
    14161 tccagtgctg actggcgcgg tagataaagc gaaaaacgca ggtaaagcag tacacattat
    14221 gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg cgatggtaga
    14281 actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg acggtcgcga
    14341 caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat ttgctgcgct
    14401 gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc gcgataaccg
    14461 ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcgcagggtg agttccaggc
    14521 cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg acgagttcgt
    14581 gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag acggcgatgc
    14641 gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa atcactcgtg cattcgtgaa
    14701 cgctgatttc gatggcttcg cgcgtaagaa agtggttaac gtcgatttcg tgatgctgac
    14761 cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc tggttaacac
    14821 cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg aaaccgaaaa
    14881 atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca aaggcgaaga
    14941 tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg aaatgagctc
    15001 cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg acaccattat
    15061 ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag cggcggttaa
    15121 agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg agtccgtggg
    15181 tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg atccggcaac
    15241 gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttatg ttggtgataa
    15301 gaacgtgaaa gcggttgaag gcggcaaact ttctgacatc gcgccgacca tgttgtcgct
    15361 gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg tggaataatc
    15421 cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac cgcgcaggtt
    15481 tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt tgtgcgcctt
    15541 ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata tcgccgcaaa
    15601 agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac aattgaaaaa
    15661 gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa acacgctcaa
    15721 tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg agcagcaaaa
    15781 agccgcccag gagcgcagcc tcgccgcgca actggatgcc gcattccgtc agggcgagca
    15841 taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc gtttacaggc
    15901 ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga agcaaacgcg
    15961 tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa caaagcgagc aacaaacgct
    16021 gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg agcgtaaaaa
    16081 gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa gtgaactgcg
    16141 tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgcg gaagccgcgg cgaaagcgcg
    16201 tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgac cgccagaaag aagcgacgcg
    16261 caaaggcacc acctataaac cgaccgaaag cgaaaaatcg ctgatgtccc gaactggtgg
    16321 cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc gggccgacgc tgcatcgcta
    16381 tggtgaacag ctacagggcg aactacgctg gaaaggaatg gttatcggtg cctctgaagg
    16441 tactgaagtt aaagcgattg ccgacggtcg ggtgattctg gctgactggc tgcaaggtta
    16501 cggtctggtg gtggtggttg agcacggtaa aggcgacatg agtctttacg gctataatca
    16561 gagcgcactg gtgagcgttg gttcgcaggt tcgcgcgggc cagccaattg cactggtggg
    16621 cagcagtggc ggtcagggtc ggccttcact ctatttcgaa attcgccgcc agggtcaggc
    16681 ggtcaatcca cagccgtggt tgggaagata agttttgttt ccatttcgtc gtaacgttct
    16741 tgcatttgcc gctctgttgg cgctctcctc ccccgtactt gctggcaaac ttgccatcgt
    16801 cattgatgat tttgggtatc gcccgcacaa cgaaaaccag gtgctggcga tgccctccgc
    16861 tatctccgtc gctgtattac ccgattcacc gcacgccaga gagatggcga ccaaagcgca
    16921 taacagcggg cacgaagtgt tgattcatct cccgatggca ccactgagta aacagccgct
    16981 ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa attgagcgca ttattcgtag
    17041 tgcggtcaat aacgtgccct atgccgtggg gatcaacaac cacatgggta gcaagatgac
    17101 ctctaacctg tttggtatgc agaaagtgat gcgggcactg gagcgttaca atctttactt
    17161 cctcgacagc gtaaccatcg gtaataccca ggcgatgcgc gccgcgcaag gtactggcgt
    17221 gaaggtgatc aaacggaagg tgttcctcga cgattcgcag aacgaagcgg acatccgtgt
    17281 gcaatttaat cgcgcaattg atctggcgcg tcgcaacggt tcaaccattg ccattgggca
    17341 tcctcaccct tcaacggtac gcgtgctaca acagatggtt tataacctgc cgccagacat
    17401 tacgctggtg aaagccagca gcttgctgaa tgaaccgcag gttgatactt ctacaccgcc
    17461 gaaaaacgct gtgcctgatg caccgcgtaa tccgttccgt ggcgtgaagc tgtgcaaacc
    17521 gaagaaaccg atagaacctg tttatgctaa tcgcttcttt gaagtattaa gcgaaagcat
    17581 cagccagagc acgctgatcg tttacttcca gcatcagtgg caaggctggg gcaaacagcc
    17641 cgaagcggcg aagtttaacg ctagcgcaaa ttaaaggcgc tacgcgtaat gcgatggtgc
    17701 gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc acagtaacgc ctggtaaccg
    17761 accttcacgc tgcgcacatt ggtaatcagg cgcttataca taccggaagt aaatatctcg
    17821 gcaatcatcc gttggcgggt aagaatatcc ggctctttgc gcaccgcatg gcaaacacgc
    17881 aatgcttcgt aagttatttg ctgatgaaat tccggataaa tcgtaatttt gtcggcataa
    17941 tttcgattta atttctccag caggcgggta atcttaatat agtgacgttg ataattaagg
    18001 tttttattcc cttgtctatg caaccgactc actgacgtat tatgcagata atatttatat
    18061 aatgattgct cggtatatcg cgcacgcagc gcgttaaaca tgaattctgt tgt
//
LOCUS       DAZKGS010000083        17890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKGS010000083 DAZKGS010000000
VERSION     DAZKGS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(450..1451)
                     /locus_tag="ACHOQY_002888"
     CDS             complement(450..1451)
                     /locus_tag="ACHOQY_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9678949.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1558..1854
                     /locus_tag="ACHOQY_002889"
     CDS             1558..1854
                     /locus_tag="ACHOQY_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9678950.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1988..2413)
                     /gene="arsC"
                     /locus_tag="ACHOQY_002890"
     CDS             complement(1988..2413)
                     /gene="arsC"
                     /locus_tag="ACHOQY_002890"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9678951.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2426..3715)
                     /gene="arsB"
                     /locus_tag="ACHOQY_002891"
     CDS             complement(2426..3715)
                     /gene="arsB"
                     /locus_tag="ACHOQY_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP9678952.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3769..4122)
                     /gene="arsR"
                     /locus_tag="ACHOQY_002892"
     CDS             complement(3769..4122)
                     /gene="arsR"
                     /locus_tag="ACHOQY_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP9678953.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4862..4945
                     /gene="dinQ"
                     /locus_tag="ACHOQY_002893"
     CDS             4862..4945
                     /gene="dinQ"
                     /locus_tag="ACHOQY_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9678954.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4999..6351)
                     /gene="gorA"
                     /locus_tag="ACHOQY_002894"
     CDS             complement(4999..6351)
                     /gene="gorA"
                     /locus_tag="ACHOQY_002894"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9678955.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6423..7265)
                     /gene="rlmJ"
                     /locus_tag="ACHOQY_002895"
     CDS             complement(6423..7265)
                     /gene="rlmJ"
                     /locus_tag="ACHOQY_002895"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9678956.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7468..9510
                     /gene="prlC"
                     /locus_tag="ACHOQY_002896"
     CDS             7468..9510
                     /gene="prlC"
                     /locus_tag="ACHOQY_002896"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9678957.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9518..10270
                     /gene="rsmJ"
                     /locus_tag="ACHOQY_002897"
     CDS             9518..10270
                     /gene="rsmJ"
                     /locus_tag="ACHOQY_002897"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9678958.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10319..11788)
                     /gene="dtpB"
                     /locus_tag="ACHOQY_002898"
     CDS             complement(10319..11788)
                     /gene="dtpB"
                     /locus_tag="ACHOQY_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9678959.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11954..12007
                     /locus_tag="ACHOQY_002899"
     CDS             11954..12007
                     /locus_tag="ACHOQY_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678960.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12106..12540)
                     /gene="uspA"
                     /locus_tag="ACHOQY_002900"
     CDS             complement(12106..12540)
                     /gene="uspA"
                     /locus_tag="ACHOQY_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9678961.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12931..13266
                     /gene="uspB"
                     /locus_tag="ACHOQY_002901"
     CDS             12931..13266
                     /gene="uspB"
                     /locus_tag="ACHOQY_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9678962.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13511..15010)
                     /gene="pitA"
                     /locus_tag="ACHOQY_002902"
     CDS             complement(13511..15010)
                     /gene="pitA"
                     /locus_tag="ACHOQY_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9678963.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15242..16444
                     /gene="yhiN"
                     /locus_tag="ACHOQY_002903"
     CDS             15242..16444
                     /gene="yhiN"
                     /locus_tag="ACHOQY_002903"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9678964.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16760..17812)
                     /gene="yhiM"
                     /locus_tag="ACHOQY_002904"
     CDS             complement(16760..17812)
                     /gene="yhiM"
                     /locus_tag="ACHOQY_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP9678965.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4666 a         4484 c         4580 g         4160 t
ORIGIN      
        1 cctttcattt ccacccggta atatttgtgt ataaaaaaag gcagtgatag tatcgtaaag
       61 ggaaacgtta acaactatca aaaagcgctc attttaaaaa gttattttgt tctccgatag
      121 tgtgtgaagt aatatttttg tgatgtttta gatgtgtaat aatgaaccag gttaaagtgt
      181 atttaaaaat gtgttgtatt agcgactgaa atctgccttt taaaaaggag gagggtactg
      241 agacaaagag tcattgcttc tccgataaaa tgtcagagaa gcatgattac gtggttattg
      301 tctgttgatg acttgttgac cgagccgtca tcattctcaa tgcattagtt aaatcatttg
      361 tgatttttaa ggattatcct gtctatgatt ttctacttag aaggatatgg cactaactat
      421 gcatcttata gcttactgct ggcatctatt taaaatagca attgagtcaa atagcccatc
      481 aagatagcca tacctaatat aaccgtcagg aatgcagcta tcatcggcat tctgaacatt
      541 gatttaagta atatcacttc cgtcaggctt gcgccggcac tgccgatgat taatgccatt
      601 aatgctccca gacccattcc ttttgtcatc agaacagatg ccagagggat aactgcctca
      661 gcccggatat acagcggaat accgacaacg gcgcttaatg ggatggcgaa gggattatct
      721 gctcctgcat gagcggcaat ccacgctgag ggaataaagc cataaataaa agatcctatt
      781 aatactccta acaaaagata tggcagtaca tctttaaact gttgcaacgc atctttggtg
      841 gctagtttta tgggattaat acttactaca gctttggtac tgcaacaggt aactacgggg
      901 ttctcaatgg ctttagccgc tggactacaa caactcactt ttatgggcgt atatgtcgtt
      961 cccggcgaag tttttgctgg agcacaacaa tttgctgatg agcttttact ggcaatgata
     1021 tgacgttcaa accccagaga atccaggata atactggcaa gaacggagac gccggctgcg
     1081 ataatcgcgt acaacaaggt gactttccaa ccaaaggtca tccacattag tccgacgata
     1141 atgggattaa gtaatggtga aacaaataaa aaagtgagag tcggaccaaa cccggctttc
     1201 gctgacaaca atccacgtaa catgggtatt gtcgagcaac tacagaacgg ggtaacggct
     1261 cccaacagag cagccaggag atagcctctc ccttttctgg cccccatcat ctgctggatt
     1321 ttatggtctg gcaccttttg tcttatcagg ctgacaccgg cactaatcac tataaacagc
     1381 aacgaaagct cgacggcgag aaacacaaac atctctgcgg catcttgcag catcgcaagc
     1441 caactactca tacgtaacct cattggataa atctatattt ccagaataat cgaaatgaaa
     1501 ttcagatcaa gatatttctg gtattatcga aatatcaacc agacagaggg gtgtcatatg
     1561 cagttggaag aagtcgctaa agcgctgaaa gagctaggtc acccgacccg tttgttcata
     1621 tttaaacatc ttgtcaaagc cggggaacaa gggttgccgg tcggtgagtt gcaaaagcag
     1681 cttgggatac ctggctccac actcagccac catatatcag cactggtatc ggtaggactg
     1741 gtcaaacaaa accgtgagag tcggacgctg atgtgtgttt cgcaatacgc aatgctggaa
     1801 gcgataattg aattcttacg cgaggagtgc tgcgtaaata gcaaaattgc ctgatgcgct
     1861 acgctctaca tatttcatgc catatattga atttgcacgg ttttgtaggt cggataagac
     1921 gcgttagcgt cgcatccgac ataaacaaag cgcactttac caactatcgg cagtggatat
     1981 cgccgcttta tttcaggcgc ttacccgctt catcaaccac tttctcgcca tcttccttgg
     2041 agaatgcgcc tttttgcgca tctggcagaa tttccagcac cacttctgaa gggcggcaca
     2101 ggcgagttcc cagcggcgtc accacaatcg ggcgattaat cagaatcggg tgctgaagca
     2161 taaagtcgat taaccgatcg tcagtaaatt tatcttccgc aaggcccagc tcctcatacg
     2221 gttcgacgtt tttacgcagc agcgcgcgta cggaaatccc catatcggca atgagtttga
     2281 ccagttcatc gcgcgttggc ggagtttcca gataatggat aatggtcggt tctgtgccgc
     2341 tgttgcggat catctccagc gtattacgcg acgtgccgca ggccgggttg tgataaatgg
     2401 taatgttgct catatcagta tctcattaca aagtgaaaga gagacgtagc gccagcgcag
     2461 ccagcgtcac aaacagcaca ggcagggtca tgataatccc tgtacggaag taatatcccc
     2521 agctgatagt catattcttc tgcgaaagta cgtgcagcca gagtagcgta gccaggctac
     2581 caattggggt aattttcggt cccaaatcgc agccaatcac attggcataa accatcgctt
     2641 ctttgataac gccagatgcc gtgctgccat caatggacaa cgcgccaacc agtaccgtcg
     2701 gcatattgtt cataatagaa gagaggaagg cggtgaggaa tccggtgccg agcgtcgcgg
     2761 cccacaggcc gttatccgcc agcacgttga gtacgccaga aagatattcc gttaatccgg
     2821 cattgcgcag gccataaacc accagataca tgccgagcga gaagatgaca atctgccagg
     2881 gggcaccgcg caggacttta cccgtattaa tcgcatgacc gcgtttagcg acgacaaata
     2941 atatcagcgc gcccacagct gcaatggcgc tcaccggaat gccgagcggt tccaggacga
     3001 aaaatcccac cagcagaagc agtaaaacaa cccagccagt tttgaacgta gcaggatctt
     3061 tgatcgcttc tgcgggagat ttcagcagcg ccatatcgta gttctgcgga atatctttgc
     3121 gaaaatagag atgtaacatc accagcgtgg caacaatcgc ggcgatatcc accggcacca
     3181 tcaccgaggc gtattcgcga aagccgaggc caaagaaatc agcggaaacg atattcacca
     3241 ggttggagac aataagcggc aggctggcgg tatcggcaat gaatccggcc gccatcacga
     3301 acgccagcgt agtgccttta ctgaacccta aagccagcag catggcgatg acaatcggtg
     3361 tcaaaataag cgccgcgcca tcattggcaa acagggcggc aacggcagca ccgagcagga
     3421 caatccaggt aaacagcaag cgaccacgac cattacccca gcgtgagacg tgcagcgccg
     3481 cccattcaaa aaagccggac tcatccagca gcaggctgat gataatgacg gcgataaacg
     3541 cagccgtcgc gttccagacg atattccaca ccaccggaat atcacccgga tggaccacgc
     3601 ccgtaactaa cgccagtact gcgccgagcg ttgcactcca gccgatgcct aaacctttcg
     3661 gctgccagat aaccaatacg atggtcagga caaagatagc gcctgccagt aacataatgc
     3721 ctcccggata aaacacatct gaaaattcat atgtgtttag ctaaattttt aactgcaaat
     3781 gttcttactg tccccggaac agttttgtcg agccaggttg cggacaatcg cctgaacctt
     3841 ttcctgttca catcgccagg cctcatcaat aattttcgcc gcccatgctg gaatatgcgg
     3901 tgataagcgg taatgaaccc acttaccttg cttgcggtcc agcaatagcc cgctttcacg
     3961 cagcaatgcc aggtggcggg agatcttggg ctgcgactgg tcgagagcag tgcagagatc
     4021 gcagacgcat aactctccca gttcgctgag cagtaaaacg atgcccagac gggtttcatc
     4081 agcaagaatt ttgaacaatt ggatgggtaa cagaaatgac atattgcgct cctgattgtt
     4141 gcaggtagtg tctctcttcg aagcggataa gtcaaaaaca tatatgactt aacgaatgtg
     4201 taagtgcaga ggaaggtaat aggtgtgaat tttgagttgg ctattcattt gaaaggaggt
     4261 ctgaatcagg acgtaaaaaa accgactttg cgtcggtttt ttactttcca gccctgagtt
     4321 ggtggctctg gctggagtga gagagttctt atctaacagc tcaatactat tagccagtgg
     4381 ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc ggtatatatc
     4441 gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg tccgaatccg
     4501 gatgtaaaaa aaccgacttt gcgtcggttt tttactttcc agccctgagt tggtggcact
     4561 ggctggagtg agaatgccat tatctaacgc atcaatgcta ataccatact gaaactattg
     4621 caaggacatg ctggttttat aacctgaatg tactgtatga ttatccagtt agctctgagg
     4681 cattttcact ctggtaatgc gcataaacgc tttcaaagtc ctggtcagaa gtacgggtgg
     4741 tgccgttaac tgatgctctg gccggagtga gaatgccatt atctaacaat agggcatgcg
     4801 ccgtgacagg cagtggatga gtaagcggat gcattctcac tccatcgcat ggagaaaacg
     4861 ggtgattgat aaagcaatca tcgttcttgg ggcgttaatt gcgctgctgg aactgatccg
     4921 ctttctgctt cagcttctga actgatagcg gaaacgtaat taagggctaa gagcacacta
     4981 ctcttagccc tttaacattt aacgcattgt cacgaactct tctgccgccg ttgggtgaat
     5041 ggcgacggta ttgtcgaagt cttttttggt tgcacccatc ttcagcgcca ccgcgaagcc
     5101 ctgcaacatt tcgtccatac caaagccaat gccgtgaata ccgacaatct tctcttccga
     5161 tccaacgcac accagcttca tgcggcacgg ctggcggtga gtggtgacgg cggtatacat
     5221 cgcggtgaaa gaggatttat acactttcac ctgatcgtcg ccatactgct cgcgcgcctg
     5281 cggttccgtt aaaccaacag taccaatcgg cggatggctg aagaccacgg tcggaatgtt
     5341 gctgtaatcc agatgctcat ccggcttgtt attaaacagg cgttcagaga gacggcgacc
     5401 cgctgcaact gccaccggtg tcagctccac tgcacccgtg ttatcgccca ccgcgtaaat
     5461 accttcaata ttggtgtttt gatatttatc gacgacgata tagccttttt cgttagtttt
     5521 aacgccagcg gcttccaggt tgatgttgtc attggcaggc tcgcgaccaa tcgcccaaat
     5581 caggcaatcc accgtttcac tgcgaccatc ttccagctcc agcgtcaggc taccatcggc
     5641 atttttcact accgctttcg ggatggcgtt ggtgtgcagc tgcgggcctt cggcgttcat
     5701 cacttcgacc agcgtttcgg aaatcatcgg gtcgaagctg cgcagcggcg catgtttacg
     5761 cacaaacaga tgcgttttcg cgccgaggcc gttaatcacg cccgccagct caacggcgat
     5821 gtaacccgcg ccaacaaccg ccacgcgctc tggcaaagca ggaagggcga agaagccatc
     5881 agaatcaata ccgtattcca cgcccggaat atccgggtgg ctcggacgac cgcctgtggc
     5941 gatcagaata tgatcggccg tgatggtttc gccgtttacc tccagcgttt tggcatcaac
     6001 gaagcgggca aagcctttga ttacatcaac gttattttta ccgagcacgt tttcatagga
     6061 agtatgaata cggtcgatat aggcggtacg gctggcgatc aacgtttccc agttgaattt
     6121 attgatagtg gtatcaaaac cataatccgg gccgtacata tggatcgctt cacggatttg
     6181 cgccgcgtgc cacatcactt ttttcggcac acagccaaca tttacgcagg tgccgcccag
     6241 ctctttggct tcaatcagcg cacatttctg gccgtacata gccgcgcggt tgatggaggc
     6301 gataccgccg ctgccgccgc cgatggcgat gtaatcatag tgtttagtca tgacaaagtg
     6361 tccttatcgt tgattaccgc gattgtagcg cgagacgtaa taggtgccag caatggctgc
     6421 aattactccg gcacgatcca gcttacggtg gcgtgcccgg tgcctgccgg aaccagtttg
     6481 ctgtgcagcc acggcagcac gttattcatc tgctgttcca gtttccacgg cgggttaatc
     6541 acaatcatgc cggaagcggt catgccacgg cgatcgctgt ctggcagtac cgccagttca
     6601 atttgcagaa ttttgcgaat accggtcgct tccagatcgt ggatcatgcg cttaatttgc
     6661 tgacgcagca ccaccggata ccacagtgcg taagtaccag tggcgaaacg tttgtaacct
     6721 tctgctatcc cgctgaccac cgcctgatag tcggttttca tttcatacgg cgggtcgata
     6781 aggattaaac cacggcggga aaccggcggc agtttggcct taagctgctg gaaaccatcg
     6841 gctttttcga cgcgcgcacg gctgtctttc tgaaattcag aacgcagcaa cgggtaatcg
     6901 ctcgggtgca gttcggtcag ttgcaggctg tcttgttcgc gcagtagctg gcgagcaatc
     6961 aatggcgaac cggggtagta acgcaactga ccgctacggt tgaagtgttt taccacattg
     7021 atgtacgcct ccagttctgc gggcaaatcg tcctgctgcc agatacgggc gatgccttcg
     7081 agatattcgc cggtacgctc ggcatgttcg ctgcctaact gataacgccc ggcccctgcg
     7141 tgggtgtcga gatagagaaa cggtttatct ttctctttca gcgactcgat gatcaggctc
     7201 tgaacggtat gtttaaggac gtcggcgtgg ttgccagcgt gaaagctgtg gcgataactg
     7261 agcatgggta aaggtgttcc ggtaagtaaa aatcggcccg tattcggggc gcacaaaagc
     7321 gtatcaggac agtataccga aaagaggccg ccgccgcgaa agcgtaacgt ttctcattga
     7381 aattcactac acttaacccc atgctacaca cattatgtaa agcgcctgtt gagcgcttcc
     7441 ttaacctctt taaccaggac tgcgcgaatg acgaatccgt tactgactcc ctttgaattg
     7501 cctccgtttt ctaaaattct cccggaacat gtcgttccag ccgtgactaa ggcgctgaac
     7561 gactgccgcg aaaatgtgga gcgcgtagta gcgcaaggag caccgtacac ctgggaaaat
     7621 ctctgccagc cgttggcgga agtggacgat gtgctggggc gtatcttctc cccggtcagc
     7681 cacctgaact cggtgaaaaa tagcccggaa ctgcgtgaag cgtacgaaca aaccctgccg
     7741 ctgctgtcgg aatacagcac ctgggtaggg caacatgaag ggctgtataa agcgtatcgc
     7801 gacctgcgcg atggcgatca ttacgccacg ctgaacacgg ctcagaaaaa agcggttgat
     7861 aacgcactgc gcgacttcga actctctggc atcggtctgc caatagagaa acaacaacgc
     7921 tacggtgaga ttgccactcg tctttccgag ctgggcaacc agtacagcaa caacgtcctc
     7981 gatgcgacga tgggctggac caaactcgtt accgacgaag cggagctggc ggggatgcct
     8041 gaaagcgcgc tggctgcggc aaaagcccag gccgaagcga aagagctgga aggctacctg
     8101 ctgacgctgg atatcccaag ctacctgccg gtaatgacct actgcgacaa ccaggccttg
     8161 cgtgaagaga tgtatcgcgc ttacagcacc cgcgcctccg atcaaggccc gaacgccggt
     8221 aaatgggata acagcaaggt gatggaagag atcctcgctc tgcgtcacga actggcgcaa
     8281 ctgctgggct ttgaaaacta cgcctttaaa tcccttgcca ctaaaatggc agaaaacccg
     8341 cagcaggtgc tggatttctt aaccgatctg gcaaaacgcg cgcgtccgca aggcgaaaaa
     8401 gagctggcac aactgcgcgc cttcgccaaa gcggaatttg gcgtcgatga gttgcagccg
     8461 tgggatatcg cgtactacag cgaaaaacaa aaacagcacc tctacagcat cagcgacgag
     8521 cagctgcgtc cgtacttccc agaaaacaaa gcggttaacg gcctgtttga agtggtgaaa
     8581 cgtatttacg gcatcaccgc taaagagcgt aaagatgttg atgtctggca tccggatgta
     8641 cgtttcttcg aactgtatga cgaaaataac gaactgcgcg gcagcttcta cctcgatctg
     8701 tatgcccgtg aaaacaagcg cggcggggcg tggatggatg actgcgtagg ccagatgcgt
     8761 aaagctgatg gttcgctgca aaaaccggtc gcgtatttga cttgtaactt caaccgcccg
     8821 gtaaatggta aaccggcgct gttcactcac gaggaagtga tcaccctgtt ccacgagttc
     8881 ggtcacggcc tgcaccatat gctgacccgc atcgaaaccg ctggtgtttc cggtatcagc
     8941 ggtgtgccgt gggatgcggt cgaactgccg agtcagttta tggaaaactg gtgctgggag
     9001 ccggaggcgc tggcgtttat ctccggtcac tatgaaaccg gcgaaccgct gccgaaagag
     9061 ttgctggata aaatgctggc ggcgaagaac taccaggcgg cgctgtttat tctgcgtcag
     9121 ctggagttcg gcctgtttga tttccgcctt catgccgagt tccgcccgga tcagggggca
     9181 aaaatcctcg aaactctggc agaaatcaag aaactggttg ccgtggtgcc atctccgtcc
     9241 tggggccgtt tcccgcacgc tttcagccat attttcgccg gtggttatgc ggcaggttac
     9301 tacagctacc tgtgggctga cgtactggcg gcagatgctt tctcgcgctt tgaggaagag
     9361 ggcattttca accgtgaaac cgggcagtcg ttcctcgaca acattctgag ccgtggcggt
     9421 tcagaagagc cgatggatct gttcaaacgc ttccgtggtc gtgaaccgca gctggatgcg
     9481 atgctggagc attacggcat taagggctga tcattcagtg aaaatctgct taattgatga
     9541 aacaggcgcc ggagacggcg ccttatctgt tctggcggcc cgctgggggc tggagcacga
     9601 tgaagacaac ctgatggcgc tggtgttaac gccggaacat ctggaattgc gcaagcgtga
     9661 tgagccaaaa cttggcggca tatttgttga ttttgttggc ggagcgatgg cgcaccgacg
     9721 caaattcggc ggtggtcgcg gtgaggcggt ggcgaaagcg gtgggcatta aaggcgatta
     9781 tttgccggat gtggtggacg ccaccgcagg gctggggcgc gatgcctttg tgctggcttc
     9841 agtcggttgc cgcgtgcgga tgctggagcg taatccagtc gttgccgcat tgctcgacga
     9901 cggcctggcg cgtggttatg cggatgcgga aatcggcggc tggttgcagg agcgtttgca
     9961 gttaattcac gcctccagcc tgacggcgct aaccgatatt accccacgcc cgcaggtggt
    10021 ctatctcgac ccgatgttcc cgcataagca gaaaagcgcg ctggtgaaaa aagagatgcg
    10081 tgtctttcag tcgctggtgg ggccggatct tgatgccgat ggattgctgg agcctgcacg
    10141 cttgctggcg accaaacgcg tggtagtaaa gcgcccggac tacgcgccac cgctagcgaa
    10201 tgtcgccacg ccaaacgcgg ttgtgactaa agggcatcgc tttgatatct atgcaggtac
    10261 gcccgtgtaa aagtaaagca gggtagcgat aacgctaccc tgctttctgc aagaataatt
    10321 aatggctttc cggcgtcgca atcatgcgtt tcagccacgg caccatcaac agcatcacta
    10381 ctgcaacgcc cagcgtgacc agaccaatct taccaaacac gttggtatag acgggcaacg
    10441 tctcaagcgg atcggtaatg ttgtccggca ccgcggtaaa tgttgccaca tagccgccca
    10501 gcaagaacgc ggcagcctgc gtcaggaacc acatcccgag aataaagccc atcaaatgct
    10561 gcggcaccag ggcagcaatc atcgccaggc caagggcgct aataaacagt tcacctaagc
    10621 tctggaataa gtacaccagc acgataaacc atggcgatgt cagcccttgt gcatccgcaa
    10681 accacattcc cgcagctgcc gccgtcaaaa agcccagtga gcacataaac atgccgagag
    10741 taaatttcat cggcatcgag aggtctttgc ctttgttacc cagatgcgtg taaatgcctg
    10801 ccagtattgg gctggcgagt accacccaga acgggttaag cgcctggaag ctgaccgggt
    10861 tgatggaaaa accgagaatt tcatgatgca cgttgttgat ggcaaagaag ttcagcgatg
    10921 ttggcatctg ggcgtagaga atgtaaaaca ccaccgcttc gagcatcagg acaaaggcga
    10981 caaacatttt attgcgcccg gttttatcca gcttgaatgc ctgacgaaag aagatgatgg
    11041 tgacgacgat ggagagaaca atcagcacca gattggcgac ttctacgttg tgcatcagcc
    11101 atgcacatac gaagatcatc accacgctgc caagtaatac gtacaacagt ttgctgaagc
    11161 tcatcggctt gaagtcgggt tcagaaccaa tgtctttcac cattccacga caggcgatgt
    11221 aaaccagtaa tgcgataatt aaccccgccc cgcacaggtt gtaggtgact gaataaccga
    11281 atctatcagc gatcacaggg gccagcgata acgctatcaa cgagccgata ttgatcgaca
    11341 tatagaacag ggtgaatgcg ccatcaagcc gcggatcttt cggcggatag cacttcgaaa
    11401 gcaagctggc tgggttagct ttaaacaggc cgttaccgac agcgatagtc cccagggcga
    11461 tgaaaatcag gtcaggctta agtagcgaca tgccggtcat gaagtagcca atcgccagca
    11521 caagtgctcc gagaacaatg gtgcgtttgg tccccagcag gtggtcgccg acatagccgc
    11581 caatggaaat gaggccatag accagcgcag caaaagcacc aaaagtgaca aaagcctgct
    11641 cttgcgagaa tccaagctgt ttaacgaaga aaaccgccag tacgccctgc acgccgtagt
    11701 agccgaatcg ctcccataac tcgacaaaaa agatcatgaa aaatgggcga ggttgctgca
    11761 gcatccccat gggtgttgtt gtattcatat ttattaacct tccaatacca tcctgaaaag
    11821 tatgaacgcg taagggataa cgcgcgaaaa agtttacatc tgtcagagat tcttaatatt
    11881 gatgcggtga atagattaat acactattgc aacagaaaat aaccagctga tcacactaat
    11941 gatggaaaat atgatggtgt taaatgttgt gttggcagga gattttgatt gcgtacgcag
    12001 atcgtaggcc agataagggg tttacgctgc atcaggtaac aaggcgcgcc aaaaagcaaa
    12061 acgcccgcat atgcgggcgt tcaggaacac gtcgtggagg gatgattatt cttcttcgtc
    12121 gcgcagcgga acaatcagca tatcaacgtg aacggtgttg atcagctgac gtgcggaaga
    12181 catcagtttg ctccagaagt cctggtggtg accacaaacc accagatcca tatcgtattt
    12241 cttgattgca tcgaccagaa cctggcccag gtcgccgctg ccgctcaggg tttcagtgat
    12301 tgggtagcct gcattagtgg aaagctcggt cagcgcatga tgtgtctctt cagagatgcg
    12361 tttctgcata tcacccagat tcacatcaat aagcccggtg tataggtcag agtagtttac
    12421 atctacgtgg atcagagaaa ctttcgcatt gtaggggcga gccatagaga ctgctttctc
    12481 taccagaact ttgctttccg gggagagatc gactgcgatg agaatgtgtt tataagccat
    12541 agtgttactc cttccataaa gttgtcgatg actggccagc tagcgtttct tgtgccgtta
    12601 cgtcacccgc gtcctgcgaa ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt
    12661 taagaccatc cttaccttat agcgacccgg atgatccgtc aatccgcctt gcttacccgt
    12721 tagataaaca aattttcgac atgaatatat tgatagtggt taaccttctg gaaaaaaaac
    12781 aacctgatct cctacactat ctatagagcc gctcggatgt tggtcaccac aaaagcggtt
    12841 tctccaggac ctggtgttaa ctgattggca gcgtatcggg agcggtagcc ccggggtgga
    12901 actccgcggg caggtcgccg gggaggaggt atgataagca ccgtcgcatt attttgggct
    12961 ttatgtgtcg tttgcattgt taacatggcg cgctatttct catcactacg cgcgttgtta
    13021 gtggtactgc gtaactgcga tccattgctc tatcaatatg ttgatggagg gggctttttt
    13081 acctcacatg gccaacccaa caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt
    13141 tatcgcgatc atcacgatga tgaatttatt cgccgctgtg agcgggtgcg tcggcagttt
    13201 attctgacca gcgcattgtg tggtctggtg gtggtcagcc tgattgcatt gatgatttgg
    13261 cattaagcac ttcagcaata ataacgcata aaaaaagcgg gccagtaaac tgacccgctc
    13321 gagactgatt acaaacgcaa aattgcctga tgcgctacgc ttatcaggat ctacataatc
    13381 tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca cgccgcatcc
    13441 ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc gctcgttttg
    13501 gtgtgtacga ttacaggaac tgcaaggaga gccagtacag tccgccggaa agcagtaccg
    13561 cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta cgctgtaagc
    13621 cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga gtagtggaaa
    13681 ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc tgggccgaca
    13741 tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc gttgccacac
    13801 ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg atgatccaca
    13861 ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac aggcgctgat
    13921 cgtcagcact cacaccaggc atcttcacca ctttgtcgat agtgtcagaa acgcacagca
    13981 taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt tccacatcgg
    14041 tagtcaacat gcctttcagg cggttgagcg cgttaatggt attcgacgga tggcaatgga
    14101 actccgcggg ttgcgttgcg ccagcttccg gagccggtac taactgatca gcaccggtag
    14161 cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg atggcatcac
    14221 gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct gctggtgcga
    14281 cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc gcgccgtgcg
    14341 aaaacgccac gccgatagcg gaaaggatca gcgcaatacg cgtccagaac ggcggctttt
    14401 tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg cgtttcttgg
    14461 tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc aggccgacaa
    14521 taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg agtgcatcca
    14581 ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc gcgccaatca
    14641 gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc cagataatcg
    14701 ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata ttaagcagca
    14761 gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc agcaaaacac
    14821 ccaaaaagtt gaacaccgct gccataacca cggcgagctg agaacgcatc gcgcgggtat
    14881 agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct tcgtagaaca
    14941 gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg ccagcaaaca
    15001 aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt atcagtgact
    15061 ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg tatggatttc
    15121 actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt aatattgatg
    15181 ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag aggagcgcaa
    15241 cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt tctgttctgc
    15301 gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa aaccagggcg
    15361 caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg tcgaaccagg
    15421 cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcgcgtt ttacccagtg
    15481 ggatttcatt gatctggtca ataaacacgg catcgcctgg cacgagaaaa cgttaggaca
    15541 actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg agtgcgagaa
    15601 gggcaatgta accttcagat tgcgtagcga agtgctgagt gtggcgaagg atgaaacagg
    15661 cttcacgctt gaactgaacg gcatgactgt cggttgcgaa aagctggtca tcgcgaccgg
    15721 tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg ccgaacaatt
    15781 tggcctcaac gtgctgccga cccgcgcggg tctggtgcca ttcactctgc ataaaccgtt
    15841 gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta ccgctgaaaa
    15901 cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg gaccggcggt
    15961 gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc tgctaccgga
    16021 tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc agagcctgaa
    16081 aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc aactcgggca
    16141 aatcccggat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac tgattagcac
    16201 attgaccgac tggcgcgtac aacccaacgg cactgaaggc tatcgcactg ccgaagtgac
    16261 gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc gcaaagtgcc
    16321 tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg gctataactt
    16381 ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag cgaagtcgtc
    16441 ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag ctaattttga
    16501 tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct ccaggcttaa
    16561 tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt tataaaatat
    16621 gaacaggttg aaaaaaacag taactttgtt gtttttttat ccttaaccac tatgcattaa
    16681 tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt
    16741 tttatccggt acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg
    16801 agaacaacgt aaagcatacc gctcctaaac cgaccaaaac ccgcgaagga ataaaatatc
    16861 ccatgtcggt ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg
    16921 tgaagacggg aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg
    16981 ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa ataagaataa
    17041 tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta
    17101 ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa
    17161 acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag gcacagattg
    17221 cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca gcactacgta
    17281 acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg gcaatgaggc
    17341 aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg ctccccgctg
    17401 catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat acacaggcgg
    17461 caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc atcacatcat
    17521 tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca tagccaataa
    17581 tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa atgataataa
    17641 atgcggtagt gaataatgcc aggcatatgg ctgccagaga aatcagcaca tgacccgcaa
    17701 cgaaatactc actttgccca gaactttcca gcacaaagcc acctaacgcg atacaaatta
    17761 agcccataat taaagggatt aatttgaaaa gccacccgat atatatgttc actttaaact
    17821 cctcaggtat ccctgctaat attgaaatgt gcattaatga taatttcagc ctgttaatat
    17881 aatgacgaga
//
LOCUS       DAZKGS010000084        17850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKGS010000084 DAZKGS010000000
VERSION     DAZKGS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..311)
                     /gene="yjjY"
                     /locus_tag="ACHOQY_002905"
     CDS             complement(171..311)
                     /gene="yjjY"
                     /locus_tag="ACHOQY_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9678966.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            407..1123
                     /gene="arcA"
                     /locus_tag="ACHOQY_002906"
     CDS             407..1123
                     /gene="arcA"
                     /locus_tag="ACHOQY_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9678967.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1183..2535)
                     /gene="creD"
                     /locus_tag="ACHOQY_002907"
     CDS             complement(1183..2535)
                     /gene="creD"
                     /locus_tag="ACHOQY_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9678968.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2593..4017)
                     /gene="creC"
                     /locus_tag="ACHOQY_002908"
     CDS             complement(2593..4017)
                     /gene="creC"
                     /locus_tag="ACHOQY_002908"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9678969.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4017..4706)
                     /gene="creB"
                     /locus_tag="ACHOQY_002909"
     CDS             complement(4017..4706)
                     /gene="creB"
                     /locus_tag="ACHOQY_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9678970.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4719..5192)
                     /gene="creA"
                     /locus_tag="ACHOQY_002910"
     CDS             complement(4719..5192)
                     /gene="creA"
                     /locus_tag="ACHOQY_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9678971.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5403..6272
                     /gene="robA"
                     /locus_tag="ACHOQY_002911"
     CDS             5403..6272
                     /gene="robA"
                     /locus_tag="ACHOQY_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9678972.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6269..6916)
                     /gene="gpmB"
                     /locus_tag="ACHOQY_002912"
     CDS             complement(6269..6916)
                     /gene="gpmB"
                     /locus_tag="ACHOQY_002912"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9678973.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6968..7489
                     /gene="yjjX"
                     /locus_tag="ACHOQY_002913"
     CDS             6968..7489
                     /gene="yjjX"
                     /locus_tag="ACHOQY_002913"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9678974.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7574..7900)
                     /gene="trpR"
                     /locus_tag="ACHOQY_002914"
     CDS             complement(7574..7900)
                     /gene="trpR"
                     /locus_tag="ACHOQY_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9678975.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7990..9927)
                     /gene="sltY"
                     /locus_tag="ACHOQY_002915"
     CDS             complement(7990..9927)
                     /gene="sltY"
                     /locus_tag="ACHOQY_002915"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9678976.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            10138..11805
                     /gene="ettA"
                     /locus_tag="ACHOQY_002916"
     CDS             10138..11805
                     /gene="ettA"
                     /locus_tag="ACHOQY_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9678977.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12112..13344)
                     /gene="nadR"
                     /locus_tag="ACHOQY_002917"
     CDS             complement(12112..13344)
                     /gene="nadR"
                     /locus_tag="ACHOQY_002917"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9678978.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13365..14747)
                     /gene="radA"
                     /locus_tag="ACHOQY_002918"
     CDS             complement(13365..14747)
                     /gene="radA"
                     /locus_tag="ACHOQY_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9678979.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14796..15764)
                     /gene="serB"
                     /locus_tag="ACHOQY_002919"
     CDS             complement(14796..15764)
                     /gene="serB"
                     /locus_tag="ACHOQY_002919"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9678980.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            15870..16514
                     /locus_tag="ACHOQY_002920"
     CDS             15870..16514
                     /locus_tag="ACHOQY_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP9678981.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16542..17558
                     /gene="lplA"
                     /locus_tag="ACHOQY_002921"
     CDS             16542..17558
                     /gene="lplA"
                     /locus_tag="ACHOQY_002921"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9678982.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17590..>17850
                     /locus_tag="ACHOQY_002922"
     CDS             17590..>17850
                     /locus_tag="ACHOQY_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9678983.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         4260 a         4977 c         4498 g         4115 t
ORIGIN      
        1 ttcaccacgt ttattagttg tatgatgcaa ctagttggat tattaaaata atgtgacgaa
       61 agctagcatt tagatacgat gatttcatca aactgttaac gtgctacaat tgaacttgat
      121 atatgtcaac gaagcgtagt tttattgggt gtccggcccc tcttagcctg ttatgttgct
      181 gttaaaatgg ttaggatgac agccgttttt gacactgtcg ggtcctgagg gaaagtaccc
      241 acgaccaagc taatgatgtt gttgacgttg atggaaagtg catcaagaac gcaattacgt
      301 actttagtca tgttacgccg atcatgttaa tttgcagcat gcatcaggca ggtcagggac
      361 ttttgtactt cctgtttcga tttagttggc aatttaggta gcaaacatgc agaccccgca
      421 cattcttatc gttgaagacg agttggtaac acgcaacacg ttgaaaagta ttttcgaagc
      481 ggaaggctat gatgttttcg aagcgacaga tggcgcggaa atgcatcaga tcctctctga
      541 atatgacatc aacctggtga tcatggatat caatctgccg ggtaagaacg gtcttctgtt
      601 agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa
      661 cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat gactacatca ccaaaccgtt
      721 caacccgcgt gaactgacga ttcgtgcacg caacctgctg tcccgtacca tgaatctggg
      781 tactgtcagc gaagaacgtc gtagcgttga aagctacaag ttcaatggtt gggaactgga
      841 catcaacagc cgttcgttga tcggccctga tggcgagcag tacaagctgc cgcgcagcga
      901 gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa attcagtccc gtgctgaact
      961 gctgaagaaa atgaccggcc gtgagctgaa accacacgac cgtactgtag acgtgacgat
     1021 ccgccgtatt cgtaaacatt tcgaatctac gccggatacg ccggaaatca tcgccaccat
     1081 ccacggtgaa ggttatcgct tctgcggtga tctggaagat taatcggcgt taccaccgtc
     1141 aaaaaaaatg gcgcttttta gcgccgtttt tatttttcaa ccttatttcc agatacgtaa
     1201 ttgatcgtcc attgtaactt ctttactggc tttcattttc ggcagtgaaa acgcatacca
     1261 gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc accaccagca cactggttcc
     1321 caacaacagc gcgctatcgg cagagttgag cagtccccac atcacaccat ccagcaacaa
     1381 cagcgcgagg gtaaacaaca tgctgttgcg ccagcctttc aataccgcct gcaaataaat
     1441 accgttcatt aacgccccaa tcagactggc gattatccat gccacggtaa aaccgatatg
     1501 ttcagaaagc gccagcaaga gcaaataaaa catcaccaat gaaagcccca ccagcaaata
     1561 ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt
     1621 cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc gttaattggt attgatcggc
     1681 tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc cagccggtat cattgcctga
     1741 agcaaaacgc tcaccgagat tattagcaaa ccagctgctt tgccattgcg cctgaaaacc
     1801 tgactcgcta acttcccgtt tggctggcaa aaaatcacct aaaaaactgg gatgcggcca
     1861 gttgctggtt aaggtcattt cgctattacg cccggcaggc accacagaaa gatcgccggt
     1921 accgcttaaa ttcagggcca tattcagctt caggttctgc ttccgccagt ccccttcggg
     1981 taaagggata tgcacgccct gcccgccttg ctctaacccg gtgccgggtt caatggtcag
     2041 cgccgttccg ttaacttcag gcgctttcac cacaccaata ccacgcgcat ccccgacgct
     2101 aatcacaata aagggcttgc ctaagatgat atttggcgcg ttgagctcgc taagacgcga
     2161 aacatcgaaa tcggctttta gcgttaaatc actgtgccag acctgaccgg tataaatccc
     2221 tatcttgcgt tcttccacgt tctgattgcc atcaaccatc aatgactcag gtaaccaaaa
     2281 atggataaaa cttcgtttcc gctccacggt tttatcctct tcctgcaccg tataaagctc
     2341 ggtcacagga atagcgataa gcggcccaac gagtttttgc ggcccgctgg tactttggcg
     2401 aatcgcatct tccacatcgc tacggtaatc ggcacgttcg acaatcactt gccgaatcag
     2461 cattatcgga atcaacaaca gcaatactgc accaaacaag gtggtcattt tccagaacag
     2521 gggggatttc aacatagtct tctcctttgc aatggcagca ggatacgaag gctatgtggg
     2581 aagaatttga agctatgtga agtgacggtg aagtcgaagc gaggccagca cgccaccttc
     2641 ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc
     2701 caaccccaga ccgctgcttt tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa
     2761 aatacgtgac agcgcgtagt caggaatacc actaccggta tccagcacct taagggcgac
     2821 gtgttcctga tccacttcgg cgcttagcgt tatgcgaccg ctctcggggg taaaatcgat
     2881 ggcgttatcc agtaaattcc ccagcgcctg ctccagtaac gccggttcag cagcaacatt
     2941 aacctcagtg ggcataacat gtagagtgat gtttttttct gccaactgca cggtgcgcgc
     3001 ttcgctgacg cggcgaaata atgccgccac atcaacaaca gtcagaacga cttcctgacg
     3061 attctccagt cttgcctggc gtagtaacgt ttctaccaat gcctgcatac gcgcattttg
     3121 cgtcagaatg ttgtcggtaa aacgagccac cacttccggt ggcggacctt cgcgtaaaat
     3181 ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc tcatgggtta acgcataaac
     3241 atactgctca atatagtttt tcccttccag cttcacgcgc atactttcca gcgcctgcgc
     3301 aagcttacgc aactcgctac tgccgagttc aggaagagga acgggcttat tgtcagtgac
     3361 ggaatcggca tagcgagtga gccgggcaat ggagcggttg atccaccaaa ccatgcctgc
     3421 gccaatcacc agtgcaatcc ccagcaaaat ggcgctggcc cataaaattc gccgctcgct
     3481 acgcttaatg accggagcca tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag
     3541 ccgcgagccg tccataatcg gcgcggcaac atacatcacc gaactttcgg gatcggcagg
     3601 attttgcaac gtgctgcgcg caccatactg accacgcaac gttagccaga cgtcattcca
     3661 gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg aacaatactt taccctgcga
     3721 atcggtcata tagacatgat attcattgcg cactttgtta atgccaccga tattggcgcg
     3781 aaacgggcga tgttgtagct gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc
     3841 agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt gcggtatcga ttaacgtccc
     3901 ctccgttgct cttcgcacgc ccggtttaac ttctttgaca aaaatagcca gtacgaacca
     3961 ggccgccacc gccaccagta aaaaatagcc cagcagcaac cgcatgccga tacgcattac
     4021 aggcccctca ggctatatcc catgccgcga tgagtattaa tcggtgaaag atcggggttg
     4081 atggcgcgca gcttggcacg caacgtttta atgtgggtat caatggtgcg atcgtaggtg
     4141 tcctgcgcat cttcccacac gctatccatt agttgctggc gggaccagac gcggcctggc
     4201 gacttgagta acgtcttcag caataaaaac tcataccgcg tcagcgttaa tggcgtgtca
     4261 aaccagctga tctgcgccgc gggttcattc aattcaaaat gaccaatacg gatgacggga
     4321 gacggcgacg agaacttctt cacccgacgc agtaaggtgc gcacccttgc gcacacttcg
     4381 cggggtgaaa acggtttagc cacatagtcg tcagcgccaa tttccagccc aagcaggcga
     4441 tcgacctctt cacttcgggc cgtcaggaac agtacaggta acgccggatg gagcgccagt
     4501 aactggcggc ataattcaaa gccgctgata tcaggcaaac caacatcgag aatcatgacg
     4561 tcgggagcct gctggcgagc tttatccagc accggcaagc ctcgctcaaa gacctcgacg
     4621 gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc
     4681 actaaccaga ccgtttcccg ttgcataaat cgcctctgtt attgccgcca cggcatgaca
     4741 ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga
     4801 taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga cctgtaacga cttaaagacc
     4861 agggacgtgc gttttttgaa tactacctcg ccctgagctt tgccgttttt aatacgatcc
     4921 gacagttcaa tcggcccgac ttgctgacaa gaaatagccg catcggaggt atcttccgcc
     4981 agacccaatc cccctttaat acccccggtt ttcgcccggc tcacataaca ggtgacattt
     5041 ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt gatccgggcc gatcatttta
     5101 aatacggtgt cgaccgaacc aatctcctct gcatgagcca atggccccag cattatcagg
     5161 cttaaagaca ggatcaaatg cttgtatttc atattgttac catttttatc tattacacga
     5221 gcaattagtt cgtcacggta aataacatat tgattaagaa caaaatctca atacttttat
     5281 ttccgtcaag ccctaaaaca tactctacta agaaaaaaac actgaatgct aaaacagcaa
     5341 aaaatgctat tatccaatta cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt
     5401 ttatggatca ggccggcatt attcgcgacc ttttaatctg gctggaaggt catctggatc
     5461 agcccctgtc gctcgacaat gtagcggcga aagcaggtta ttccaagtgg cacttacaga
     5521 gaatgtttaa agatgtcact ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt
     5581 cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat tctggacatc gcgctgcaat
     5641 accgcttcga ctctcaacag acatttaccc gcgcattcaa gaagcagttt gcccagactc
     5701 ctgcacttta ccgccgttct cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc
     5761 tgggtgaatt cactatgcca gagcacaaat ttgtcaccct ggaagatacg ccgctgattg
     5821 gtgttaccca gagctactcc tgttcgctgg agcaaatctc tgatttccgc catgaaatgc
     5881 gttatcagtt ctggcacgat tttctcggca acgcgccgac cattccgccg gtgctctacg
     5941 gcctgaatga aacgcgtccg agtcaggata aagacgacga acaagaagta ttctatacca
     6001 ccgcgttagc ccaggatcag gcagatggct atgtactgac ggggcatccg gtgatgctgc
     6061 agggcggcga atatgtgatg tttacctatg aaggtctggg aaccggcgtg caggagttta
     6121 tcctgacggt atatggaaca tgcatgccaa tgcttaacct gacgcgccgt aaaggtcagg
     6181 atattgagcg atactacccg gcagaagatg ccaaagcagg agatcgccca attaatctgc
     6241 gctgtgaact gctgattccg atccgtcgtt aacgctgcaa ctcatctaat gcaggggcgt
     6301 ccagatgcga gatgtcccct gcggtttcaa caacccagcc agacgccagc cacaggcttt
     6361 cctgataatc cacgcgcgaa atagaacagt tacgcagacg taagcggcgc tctgcccatg
     6421 ctggtaatcc gagaatcgta ctcaccaggc atcccagtgc aataccatga ctcaccagca
     6481 gcggtcggct tccctgcggt aagtcccggc aagattccag tgccgcattg acgcgatcgc
     6541 tgagctcctg cattgactcg ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct
     6601 gccgacgcca gttctcttct tcttcggtca gagaatcgat atgtcttttt tccagcacac
     6661 ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc gcagccgcag gcctgggcga
     6721 tgatttccgc cgtacgccgg gtgcgtccta aatcgctact gatgatatgc gtaatgccaa
     6781 gctctttggc acgggttgcc acctgcatcg cctgttgctc acctttggcg gtcagcgggc
     6841 tgtcagactg gccctgaata cgtcgctcgg cgttccactg cgtttcaccg tggcggacta
     6901 ggtatacctg taacatgctt tttttccgta tactgctgta acgttaattt tctgagtaat
     6961 gctgattatg caccaagttg tctgtgcgac caccaatccc gctaaaattc aggccattct
     7021 gcaggcattt cacgagatct tcggcgaagg atcctgccat attgcatccg ttgccgtcga
     7081 gagcggtgta ccggaacaac cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg
     7141 ggtagcaaat gcccgccgtt tacttccaga ggctgatttt tgggtggcga ttgaagctgg
     7201 catcgatggt gacagcactt tcagttgggt ggtgattgaa aacgccagcc agcgcggcga
     7261 ggcgcgttcg gctactttgc cgttgcctgc ggtgattctg gaaaaagtgc gtgaaggcga
     7321 ggcgctcggc cccgtgatgt cgcgttatac cggtattgat gagattggtc gtaaggaagg
     7381 ggcgatcggc gtgtttaccg ccgggaaact cacccgcgcc agcgtctatc accaggcggt
     7441 gatcctggcg ctgagtccgt ttcataatgc cgtgtatcag gcattgtagg acggataagg
     7501 cgttcacgcc gcatccggca ttcggtgcac gatgcctgat gcgacgctgc cgcgtcttat
     7561 caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactgg cgcagctcaa
     7621 cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgccc gcgccgagtt
     7681 cattttttaa ctcacgctgg ctcatttcgc cgcgcaacag ctcttcgaca atacgcacgc
     7741 gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac ggtaaatgga
     7801 gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc tggtgacgct
     7861 gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc cattgttagc
     7921 gggggaagca aaatgcctcc cccggttgta ctcgctaaag agtacgatag catatcataa
     7981 acgtgcggat cagtaacgac gtccccattc cgtggtgctc atcaacgtcg gtttatcccc
     8041 catgaaatag cggtagtaag cgtcataagc cagcacgttc ttcacataac cgcgcgtctc
     8101 ggagaacgga atactctcga caaatgccac agcgtcgata cgcccggcgc tgttgccaag
     8161 ccaggttcgc acccgtccag gtccggcgtt ataagctgct gaggagaaaa tacggttatt
     8221 gccaaactgc tgataaacat attgcaggta actggtgcca atgttgatat tcgtttccgg
     8281 atccagcaat tgcccagggc tgctataacc aggaatagag aacatcttca ccgtatgggt
     8341 cgctgtacca ggcataatct gcatcaggcc gctggcccct accggtgatt tcactttcgg
     8401 attccaggcg ctctcctgac gggcaatcgc catcgcatag ctttgcggga tctctttccc
     8461 actggtgtaa cgtttgaaaa gatcgttgta agccagcggg aatcgctctt ccagatgatc
     8521 ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt tattgaaagc
     8581 ataccgagcc agttgagcct gctctgtttt tgacttgctc ttcaccagat tggcccactc
     8641 gctacgcgcg gtgttatcga gattccagta catcaactcg cgcacgcgcg ccatctccgg
     8701 cccctgagtc agggcgctgt caacattctg cggcgcttta tcaatcttca gctcatactc
     8761 ttctccgatg cgttgtgctg caaccatcgg gtagaaacca cgctgttgca tgagttgatg
     8821 caaaatctct ttcgcttcag cttcacgtcc gcgttccagc aataaatccg cctgccagta
     8881 acgccattca tctttctctt ttgcttccat cggcagacgc gccagccagg tattcaggcc
     8941 gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt tcaatcaacg acgtggattg
     9001 cgagcgcata atggcgtcat cgcgccattt cgcctgctcg tcggtgacat cgttgcccat
     9061 caaacgccag gcgacgatat cgcgcagctc ctgaatatga tcttcattaa gctgctgcgc
     9121 ctgggcaagc gacgggatca tcagccgtgc attttcagca tcctgccgcg ccacactggc
     9181 aaacgccacc gccgccattt gacgggtaaa atcggtcgct ccggttgtac gcgcgaaggt
     9241 cagtaccgta ttagggttgt tcgccagtga aatgattgcc gaggcgatag tctggtaatc
     9301 ggcaggcatc tgccctgcca gcactgttac caggccagtg ttacccgctt tcatcgccag
     9361 acggatacgc tctaaataag ccagcggatc ttgtttacct gacgcacgcc agacgctaaa
     9421 taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt tcgccccttg
     9481 ccaggcttct tcactctgcc cggtgttcca tttcgcatag tagtaattac attgcgcttc
     9541 ggtagttccg ggcttttccg ggctaaaggc taacaagcca cgccagtctt cacgccgcgc
     9601 cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg
     9661 aacaaagtta gtgaccgtca ccgccggttg attcatcaga tcatcggtga tctggcggta
     9721 ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt cgaccacatc
     9781 catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa cggctacgct gctcatccag
     9841 tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag ccaacagccg
     9901 ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat acatcaatgc
     9961 agcgtcatgc cgcgtaatag atttatgcta accagtcatt gccgtttacg ccacgttacg
    10021 gacacttttt tacttttact gcgaaggtga tcgaccaact gggattaggc ggggaaaaag
    10081 gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa gtccaacgtg
    10141 gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt
    10201 ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac
    10261 ggcgcgggta agtctaccct gctgcgcatt atggcgggca ttgataaaga catcgaaggt
    10321 gaagcgcgtc cgcagccaga catcaagatt ggttacctgc cgcaggaacc gcagctgaac
    10381 ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt ctgaagtggt taacgccctg
    10441 aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt tgacaagctg
    10501 gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca taacctgaac
    10561 gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg actgggacgc gaaaatcgct
    10621 aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc gcctgctgct ggaaaaacca
    10681 gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt ggcctggctg
    10741 gaacgcttcc tgcacgactt cgagggcacc gtggtggcga ttacccacga ccgttacttc
    10801 ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg gtgaaggtat tccgtgggaa
    10861 ggcaactact cctcctggct ggagcagaaa gatcagcgcc tggcgcagga agcttcacaa
    10921 gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca gggaactaaa
    10981 ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg aagagctgaa cagcaccgaa
    11041 tatcagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg tctgggcgat
    11101 aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg atcgcctgct gattgatgac
    11161 ctgagcttct cgatcccgaa aggggcaatc gtcgggatca tcggtccgaa cggcgcgggt
    11221 aaatcgaccc tgttccgtat gatctctggt caggaacagc cggacagcgg caccatcact
    11281 ttaggtgaaa cggtgaaact ggcatcggtt gatcagttcc gtgactcaat ggataacagc
    11341 aaaaccgttt gggaagaagt ttccggcggg ctggatatta tgaagatcgg caacaccgag
    11401 atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca gggtaaacgc
    11461 gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt
    11521 ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga aaccctgcgc
    11581 gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc gcacgaccgt
    11641 tggttcctcg accgtatcgc cacgcacatc ctggactacc aggatgaagg taaagttgag
    11701 ttcttcgaag gtaactttac tgagtacgaa gagtacaaga aacgcacgct gggcgcagac
    11761 gcactggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt aaaatgccgg
    11821 atgcggcgtg aacgccttat ccggcctaca aaccaagaaa tttcaatacg ttgtgaatct
    11881 ccaggcctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa tgccggatac
    11941 ggcgtgaacg ccttttccgg cctacaacag ccttagtatc cgcaatttcg tagcgggtca
    12001 cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc ctcttccaga aaggttttca
    12061 actcatcaaa aaacttattt tatacctcgc ctttgagccg tttcatcgcg gttatctctg
    12121 ctcccccatc atctcccgca ccagctccac gcagcgcagg aaacggctgt cataatcgtc
    12181 ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc atctccacca gcaagttctg
    12241 gaactctttg cgatccaccg aactgccgag gctgcgtaaa ccatccgcca cccacggcgt
    12301 gttgttctcc agcaggatca ccagatcgaa acggtattca tcaatcaacg cctgcacgaa
    12361 cggatgctca cgcccttcgt actttttgca gaacgcctgg gtagtgacaa aatcagtgtc
    12421 aataaacgcc actttattgg cgtatttcac tgcaaaatca atgtattgtg cgtggcccag
    12481 cgcgatttta tcgtagtcgg aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa
    12541 gacataatcg cgaccatatt cccacgcact ggtggtattg aagatattgg caagtttgtt
    12601 taccagggtg gatttaccgc tcgactcgcc gccaaggatc gccacggtac gcacaaagaa
    12661 cggcttcact tccgtaggaa tatattccca gtagcggaac gggttttcgc ggatctgcgc
    12721 accgctgata ctcataaagg tacgtttcgg atcgaccagt accgtctcga tccccagatg
    12781 ttccatatac tgtggcgcat cggcttcttc cgaggtgtag atcaggtccg gctggatccc
    12841 cttttcagcc ataaactttt tgatgccgtt gctccacaca tcccagccat gcggatacgg
    12901 ctccatgccc tcttcgttga aagcatgaat gcgaatattt ttctgatatt taaaagtttg
    12961 caataaccag cgcagacgat ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa
    13021 caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata tgcagctcgt caacctggct
    13081 acaggcgcgc tggataaggt agatatgtcc ggtatgcagc gggtagaact taccgaatac
    13141 gacaccaatg gttttcttct gccggggaaa ttcaagcccc aaaaaacggt gcaacgcctc
    13201 cagcttttgc gcgctggggc ttttaatttt ggcattcagt aactggctta aatacccttt
    13261 ggtcataccg ctggcatcag ctacctgctg taacgtgcag ccctgttgct tgatggcagt
    13321 tttcaggtaa tcaaatgacg acatatctcc ctccgtatct ctcattataa gtcgtcgaac
    13381 acgctaagcg cgtcggagag ttttttaacg ccaaaaatct gcatcccttc cggcgctttt
    13441 ttcggcacgt tagccgccgg aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagag
    13501 attcgctcct gaccgctggg caccgggcgg atctcccctg ccagcccgac ttcaccaaac
    13561 accaccagat cctgcggtag cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac
    13621 gctaagtcgg cactggtttc ggttaccttc acaccaccga ccacgttcac aaatacatcc
    13681 tgatcggcca tttgcagacc accgtgacgg tgcaagacag ccagcaggat tgccagacgg
    13741 ttttgctcca gacctaccgc cacgcggcgc ggattcgcca tcatcgagtg atcgaccagc
    13801 gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca ccaccatcac ggagctgccg
    13861 gaggtcactt catctccgcg acttaagaaa attgccgaag ggttgctgac ttcacgcagc
    13921 ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg cgccgaagcg gtttttatgg
    13981 ctgcgcaagg tgcgaaaacg ggagtcggca tcgccatcca aaagcaccga acagtcgata
    14041 cagtgttcca gcactttagg gcccgccagc gagccatctt tggttacgtg tccaaccatg
    14101 acaatcgcca caccgcgcgt tttggcgaag cgtgtcaaat aagccgccgt ttcacgcacc
    14161 tgcgccacgc taccaggcga tgactgtaca tccgccatat gcatcacctg gatggagtca
    14221 attaccatca gcttcggttg ctcttcttca gcaatcaggc agatctgctc gatgctggtt
    14281 tccgacaaca tattgagatt gtcagtcggc aggccaaggc gatgagcgcg cattgccacc
    14341 tgttgcagcg actcttcgcc ggtgacatac agcgttttca tctgctgggc cagtttgcac
    14401 agcgtttgca gcaacagcgt ggatttcccc gcgcctgggt taccgccaat cagaatggca
    14461 cttcccggta ccacgccgcc gcctaatacg cggtcgaact ctttaaatcc ggtggaaaaa
    14521 cgcggcagct cttcaaggct gatgtcggag agtttctgga cttttgccac cccggcgcta
    14581 ccggcatagc cactgagacg ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc
    14641 tcggtgatgg tgttccaggc atgacaggca ctgcactgcc cctgccagcg cggataatcg
    14701 gccccgcatt cattacaaac aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt
    14761 aatgctgtgc cgcccgcagg atgacgggca ggcaattact tctgattcag gctgccagag
    14821 aggatgcaga atacccccat caggtcagcg tgacggatgg tgacttccgt cttttcattc
    14881 acttttggct tggcatggta ggcaatcccc agccctgccg ctttgatcat cggcaggtca
    14941 ttggctccat cgccaatcgc cacggtctgc gccagcggga tttcatactc ctgcgcgagg
    15001 cgggtcagag ttttcgcttt gtactgcgcg tcaacgatgt cgccgatcac attgccggta
    15061 aatttaccgt ccatgatctc cagttcattg gctaccacgg cggtcaggcg cagcttgtcg
    15121 cgcaggtatt cagcaaagaa agtaaagccg ccggaggcaa tcgccacttt ccagcccagc
    15181 gtttccagct tgagcaccag ctgcgttaag cctggcatca gcggcagatt ttcacgcacc
    15241 tgttgcagaa tattggcatc agcgcctttc agcgtcgcca cgcggctgcg caggctggcg
    15301 gtaaaatcga gttcgccgcg catcgcccgt tcggttacct ccgccaccat ctcgcccgtt
    15361 ccggccagtt tggcaatttc atcaatacat tcaatctgga tggcggtgga atccatgtcc
    15421 atcaccagca aacccggcgt gcgcaggtgc gggattttcc ccagcggcgc gacatccagt
    15481 tgcgcttcat gggccaggcg cgtagcgcgc gcggtgagtg aacctgccag acgaatcacc
    15541 tgataatctt ccacgcacca ggcggcaaca atcaccatcg ccgcgcccag tttgctctgg
    15601 tattgggtca ggcgttgttt atccagccca cgaccataca gcagccagcc gctacgacct
    15661 gcgtggtaat ccagtggcat cacttcatca ccacttaatg aaagaggcag acccggccat
    15721 aaagagacat cttcaggcag gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa
    15781 tcgttcgaag cagggaaaat aacgcatgag gctaccttgt atccattgct tctggcaaca
    15841 ttaagtctca aattttcaaa gggtggaaga tggctcgcac aaaactgaaa ttccggctgc
    15901 atcgggcagt gattgtcctg ttctgtcttg ccttgttagt ggcactgatg cagggggcgt
    15961 catggtttag tcaaaaccac cagcgacagc gtaatccaca gctggaagaa ctggcccgca
    16021 ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat gcgtaccgac tcaccagatg
    16081 aaaaacgcat tcaggcgatc ctcgatcagt taacggatga aagccgcatc ctcgacgcgg
    16141 gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg cgaaagcgtc gaagtgcgcg
    16201 accggctggc gctcgacggt aaaaaagcag gcggctattt taaccagcag attgtcgagc
    16261 caattgcggg taaaaacgga ccgctcggct atctgcgcct gacactcgac acccatacgc
    16321 tcgccaccga agcccaacag gtggataaca ccactaacat tttacgcctg atgttgctgc
    16381 tctcactggc aatcggcgta gtgctgaccc gcacgctgct acagggtaaa cgcacccgct
    16441 ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg
    16501 agaaaaaaga gtgacccatt actacaagaa aggaaatcgt tatgtccaca ttacgcctgc
    16561 tcatctctga ctcttacgac ccgtggttta acctggcggt ggaagagtgt atttttcgcc
    16621 aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa tgccgacacg gtagtgattg
    16681 gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg gatggaagaa gataacgtcc
    16741 gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca cgatctcggc aatacctgct
    16801 ttacctttat ggctggcaag ccggagtacg ataaaaccat ctctacgtcg attgtgctca
    16861 atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg gcgtaacgat ctggtggtga
    16921 aaacagtcga aggcgaccgc aaagtctcag gctcggccta tcgcgaaacc aaagatcgtg
    16981 gcttccacca cggcactttg ctgctcaatg ccgaccttag ccgcctggca aactatctca
    17041 atccggataa aaagaaactg gcggcgaaag gcattacctc agtgcgttcc cgcgtgacca
    17101 acctcaccga gctgctgccg gggatcaccc atgagcaggt ttgcgaggtc ataaccaagg
    17161 cctttttcgc ccattatggc gagcgtgtag aagcggaaat catctccccg gacaaaacgc
    17221 cagacttgcc aaacttcgcc gaaacctttg cccgtcagag tagctgggaa tggaacttcg
    17281 gtcaggctcc ggcattctcg catctgctgg atgaacgctt tagctggggc ggcgtggaac
    17341 tgcatttcga cgttgaaaaa ggccatatca cccgcgccca ggtgtttacc gacagcctca
    17401 accccgcgcc gctggaagcc ctcgccgggc gactgcaagg ctgcctgtac cgcgcggata
    17461 tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc ggaccaggaa aaagagctac
    17521 gggagttgtc gacgtggata gcgggggcgg taaggtaact cgctgattaa aataccaaag
    17581 gaagggaata tgatcgatct ggaaaaccag gagcgggaga ttatcaattt aatgttaagc
    17641 cagcgtattt cctggctagc cgccgtgcgt atcaggcata aattatcgct tgctgaagta
    17701 agtaaaatgt taggtatctc gattaattct ctaaaacaga tcgaaaagac ggaacggctt
    17761 agctcaaaca tcaaaagcaa aatggcagaa atttatggtt gtccaccaga gttgctgata
    17821 tgcccttcct ggatgacggc tgaacataaa
//
LOCUS       DAZKGS010000085        17840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKGS010000085 DAZKGS010000000
VERSION     DAZKGS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..613)
                     /locus_tag="ACHOQY_002923"
     CDS             complement(<1..613)
                     /locus_tag="ACHOQY_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678984.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKTLVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSALPEVRLRASELIRLYPKEIIFFICVGVL"
     gene            complement(629..1033)
                     /locus_tag="ACHOQY_002924"
     CDS             complement(629..1033)
                     /locus_tag="ACHOQY_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9678985.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(1123..1260)
                     /locus_tag="ACHOQY_002925"
     CDS             complement(1123..1260)
                     /locus_tag="ACHOQY_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678986.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            1332..1535
                     /locus_tag="ACHOQY_002926"
     CDS             1332..1535
                     /locus_tag="ACHOQY_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9678987.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            1557..1907
                     /locus_tag="ACHOQY_002927"
     CDS             1557..1907
                     /locus_tag="ACHOQY_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9678988.1"
                     /translation="MKKNTNNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHTWLIEKAGIVVHRCPHNPFTGNRIFALSSGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQAAL"
     gene            1918..2196
                     /locus_tag="ACHOQY_002928"
     CDS             1918..2196
                     /locus_tag="ACHOQY_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678989.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQIIREIISEAEKSRQVNGDEPYIEIICMDSEAEIQKAD"
     gene            2208..2450
                     /locus_tag="ACHOQY_002929"
     CDS             2208..2450
                     /locus_tag="ACHOQY_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP9678990.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGAHSERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            2447..2560
                     /locus_tag="ACHOQY_002930"
     CDS             2447..2560
                     /locus_tag="ACHOQY_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678991.1"
                     /translation="MSIFISWIVLIISVVCAIGIMRIINSVKKIERFFTGE"
     gene            2647..2850
                     /locus_tag="ACHOQY_002931"
     CDS             2647..2850
                     /locus_tag="ACHOQY_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9678992.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            3174..3563
                     /locus_tag="ACHOQY_002932"
     CDS             3174..3563
                     /locus_tag="ACHOQY_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFP9678993.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASIFAAMLAHRTAGGCAS
                     LSEYSAEFNRLSALFSQEWQRVTGYQP"
     gene            3560..6400
                     /locus_tag="ACHOQY_002933"
     CDS             3560..6400
                     /locus_tag="ACHOQY_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9678994.1"
                     /translation="MSAFAWSWNEPRPAIDPARFTERRQETETDLQRAIRYYLEANKR
                     AQEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSGTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPIHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSTRENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            6477..7436
                     /locus_tag="ACHOQY_002934"
     CDS             6477..7436
                     /locus_tag="ACHOQY_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP9678995.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            7441..7752
                     /locus_tag="ACHOQY_002935"
     CDS             7441..7752
                     /locus_tag="ACHOQY_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP9678996.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            7816..8406
                     /locus_tag="ACHOQY_002936"
     CDS             7816..8406
                     /locus_tag="ACHOQY_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9678997.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            complement(8896..9942)
                     /locus_tag="ACHOQY_002937"
     CDS             complement(8896..9942)
                     /locus_tag="ACHOQY_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9678998.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(9942..11693)
                     /locus_tag="ACHOQY_002938"
     CDS             complement(9942..11693)
                     /locus_tag="ACHOQY_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9678999.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11848..12684
                     /locus_tag="ACHOQY_002939"
     CDS             11848..12684
                     /locus_tag="ACHOQY_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9679000.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLIAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QVDIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            12708..13760
                     /locus_tag="ACHOQY_002940"
     CDS             12708..13760
                     /locus_tag="ACHOQY_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9679001.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILPDTTGPIARTNSSYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            13806..14606
                     /gene="gpM"
                     /locus_tag="ACHOQY_002941"
     CDS             13806..14606
                     /gene="gpM"
                     /locus_tag="ACHOQY_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9679002.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDIPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKNKKTTRKPATRKTTATQKAKRG"
     gene            14708..15202
                     /locus_tag="ACHOQY_002942"
     CDS             14708..15202
                     /locus_tag="ACHOQY_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9679003.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            15202..15402
                     /locus_tag="ACHOQY_002943"
     CDS             15202..15402
                     /locus_tag="ACHOQY_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP9679004.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTRGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            15405..15728
                     /locus_tag="ACHOQY_002944"
     CDS             15405..15728
                     /locus_tag="ACHOQY_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9679005.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            15725..16117
                     /locus_tag="ACHOQY_002945"
     CDS             15725..16117
                     /locus_tag="ACHOQY_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP9679006.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            16114..16521
                     /locus_tag="ACHOQY_002946"
     CDS             16114..16521
                     /locus_tag="ACHOQY_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFP9679007.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            16660..>17840
                     /locus_tag="ACHOQY_002947"
     CDS             16660..>17840
                     /locus_tag="ACHOQY_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9679008.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSVLNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     T"
BASE COUNT         4609 a         4815 c         4621 g         3795 t
ORIGIN      
        1 ccaaaacccc tacgcagata aagaatatta tttcttttgg ataaagtcgg attaattctg
       61 aagcacgaag tcgaacttct ggtaaggccg aactctctgt gtgacttgat gccgattgtt
      121 gctctaacca cgacaatgca gactgtaact gagaacgagt aagatcgttt aaacgtcctg
      181 taccgaaatt gatatggcaa taccgcagaa gtttttgtcg aagtccacag tcttcactgt
      241 tacgtagtaa taaacttaca agagtcttac aggcatcatg atctttacat cgctcaagca
      301 ttgcatgcag aaaactctcc gctgttttat attgatttac tgtcatatca tcaataccag
      361 ctacaccaat ctccgcatgt actttttgcc aaataataaa cgcttcagta ttgctagctt
      421 ctgcaatagc agcaaccaag ctatttagct ccttacgctg agccttaagc aaagggcgat
      481 cgtcatcatc attattcgaa gggattgcga tattgacggt atgagaacca tcatatcgct
      541 ctatctgaat attcttttcg tgaaaatcac gcccagcaac gcgattgttt gaaccgtttg
      601 agttgacggc catgtcacct ccctactatc acctaccctt agtttcgtta tagtcacgac
      661 cagcgatacg gttattacca ccagaaatat ttaactcacg tcctgatggc tgagtttcct
      721 tttcactgat cgcacctttt aaagccccga tcaccgcgtt tttcacatct aacgaagctg
      781 ctcgaaagcg agtaatcaac tcctgctcat catcgttata agtttcaggt gagtgaattc
      841 ccaacacaac atactgaaca tcaaggccaa aacgagacag cgctgccaaa tacgcagcat
      901 caggaaagct atctcctttc tcatatctaa gctgagttag ctttttgact ccaccaatgt
      961 cgctcatggc aacttgacta agtcccaatc tttccctttc ctcacgcaac cgctgaccaa
     1021 tatcattttt catacaaaaa ccttgacagg tatctttttt gataccaaaa tgatttcacg
     1081 agctattaga tgatcacaat ataccactat gaaacaagtt cttcacgata ccagatcacg
     1141 cattccgcgt aacaccgcca caggtccaag actggcactt cggctgtccc tcgaggagcg
     1201 agccgtcatt gatgaaatgg cagctaaaga acaacgctca tcctctaaca tggcgcgcat
     1261 gatcttcctt cgcggcctag agctaaccca gaaagaacaa aacaaatctt cctgatcagg
     1321 aggctagtgg gatgtcaggt ataaccatca atatcaatgt gaatgccccc tatgtatccc
     1381 tgcagaaata tgcagagata acaggtatcc ctcttaatac atgcaaaaag atgttggctg
     1441 acggtcgaat tattatccgc cccaaacgcg ccaaaatgga aaagcctgaa gtaaaccttg
     1501 tggcgatgtt aaaagacgct ttggctaaca gctaaaacaa tgaacagagc atcatcatga
     1561 aaaaaaacac taataatcca tactccaaat ttcgtaatgg cgtagaacgc catgtacacc
     1621 acgtcgctac cagtgcatca cgtagtaaca gccgctataa cctgaacgag acgcacgcaa
     1681 caccagatgg gcacgctgta aaacaaatcg gcgaacacac ctggctgatt gagaaagctg
     1741 gaatcgtggt tcacagatgc ccacacaatc cgtttaccgg aaaccgcatt tttgcattaa
     1801 gcagcggcga taatcagttc gggcaggatt tcacattgta cgaagcacta cgcacagtcg
     1861 atcgtctgct tcgcgggcaa agttttatta aacaggctgc tttataacag gtgctttatg
     1921 actaaagacc atgcacaagg tgtatttatc cgctttattg attttcgcgg tgaactgtta
     1981 ttacgcgcat cagctattga cggagtagtt ccatctgaaa aaaacgcagc tacctacgtt
     2041 tatctgaacg gtacacgcct gactgtagag cttccgtacc agatcattcg cgaaataatt
     2101 agcgaagccg aaaaatctcg tcaggttaat ggcgatgaac cctatatcga gattatctgc
     2161 atggattcag aagctgaaat tcagaaagca gattaaaggg tgttgcgatg gataaagaat
     2221 ataaaactct catcaacaaa gcacttgagc gtttttattt tcgtttaagc gcatccggcg
     2281 ctcattcaga acgtgcagcc cgtgactcat tgaccagggc tatccggagt ctgtatgacg
     2341 tagcttttta cgctgacgat ctggatgcac ttaatgaact ttctgagctt atctgcgccg
     2401 cagaatgcgg ggagcatatt gagccgtaca aactgggaaa tatcgcatga gtatatttat
     2461 atcctggatt gttcttatta tttcggtggt ttgtgcaatt ggtattatgc gaattattaa
     2521 ttcagtaaaa aagattgaac gctttttcac tggcgaataa ccgcataaat aaaaccccag
     2581 cttaaataaa aaaatgtgaa cacaatctgc attcgcagag gtattcacac acaccaagga
     2641 ggcgtaatgg caattaagca ttttcctgtc gttcgtttca cctccagagg acgtgaatac
     2701 gaagtcgacg aacgtctgat taccacaatc gacaaacacc gctcagaaaa ggatgcacac
     2761 cacatctatc tcactgacgg cacttacttt tgcgccacta atgtggcgcg ggtgaatctt
     2821 atccgacagg tacaggatcc acgcaaatga gtaggagaag aatcactcgc agacatcacc
     2881 gaacacacct gagttcctca gcaacgctaa aggcacttat tcaaagcgag atcggtgatt
     2941 tcttcgcggg agttggctca ccaggcgaac cagaaacacc agaagcgatg cagcgtgagc
     3001 tcatgatacg catagataac acttttgatt tcttctacag catgcacgga attaaacaga
     3061 aatgaacctc aagccagcaa taactactcg tagaacgtca attcctgtaa ccgaccgctt
     3121 ctgagttttt tggcaggaag ccttcgcaca tctgtagtaa agagaattgc agcatgattg
     3181 acgctcatga cttcacaaga tgggtgcgca cacaggacac ccgtctggct cccgttcttc
     3241 agggattatt tgatctctac atccgtggtc gtgacaacag agcacgcacc acaaaaccgg
     3301 agaatgcaga caccctttat ttcacagtag acgactgcta ccgcgtggac ttcacaccac
     3361 acgggctggc gttgcactgc ctgacaccac acggcgaatc actgctggcg tattacgact
     3421 ccccggcctc catatttgcg gcaatgctgg cgcatcgcac tgctggcggg tgtgcctcgc
     3481 tgagtgaata ctccgctgaa tttaaccgcc tttccgccct cttctcacag gagtggcagc
     3541 gcgtgacggg ataccagcca tgagtgcgtt tgcatggagc tggaatgaac cacggccagc
     3601 cattgatccg gccagattta cggagcgcag gcaggaaact gaaaccgacc tgcaacgcgc
     3661 catccgttac taccttgagg caaacaaaag ggcacaggaa gaacaggaag cgaaggaaga
     3721 agcctttttc gcacaatcca ccgtgggtaa aaaactcatg gcatcccttg aggaagccgg
     3781 acagcgtgaa aagctggcac aaagcatcat cagcaagcgt caggcaacag aacaagaccc
     3841 ggtggcccgt gcttttgcca cactgaaggt gcttcccgtt tatctgcgtg aacctctgag
     3901 ccgccacctc tcttttctgc gcaagaagca ggaagccgat cgtcagaaag gcaaaaagag
     3961 ctggcaggct gaacgctacg cgcgcggaac cctgcgcaaa atattcgaac gtctggaccg
     4021 caccgacagc cgctggctga caccgggcta tcgctccctt gccggacgcg aacgcctgga
     4081 cgatttgctt tacctgccgc agctcaacaa acaccagata cagacgctgg ccaccatgac
     4141 ggcggcgatg ttcagtggca cttttgaaaa actctgcgat ggctttggcg cgaccgatgg
     4201 cgagctgacc atggatgtaa cgctgaaggc gtatcagatg ctggcccgca tggcgttaca
     4261 cctgcacgct atgcctccac attatgacgc tctgacaaca gacaaagacc ggaggaatga
     4321 accggacacg gagctgctgc cgggcgcaat ccttcgcctg acctgtgcgg aatggtggaa
     4381 acgcaaactg tggctgttac gttgcgagtg gagagaagaa caactccgcg ccgcctgtct
     4441 ggtttccaga aaaacatcac cctatctgag ccaggacgcg ttaagcgagt ttcgcgcaca
     4501 gcgcgagaaa acacgcgatt tcctgaaaag tttcatgctg gaaaacgaag acgggttcac
     4561 gattgatctc gagacagtgt attacgcggg agtaagtaac ccgattcacc gtaaggcaga
     4621 aatgatggcc accatgaagg ggctggaact tctggccgaa gcccgtggcg acagagcggt
     4681 gtttctgact gtcacctgcc cgtcaaaata ccacgccaca acagagaacg gtcatccgaa
     4741 tcccaaatgg aacggggcca ccatgcgcga ctccagcgat tacctggtta acacgttttt
     4801 tgcggcggtc cgcaagaaac tgaaccgcga cggcctgcgc tggtatggca tccgcacggt
     4861 ggagcctcac catgacggca ccgtgcactg gcatatgatg gtctttgctc atccggaaga
     4921 aatcgacacc attgtgtccc acacccgcga tattgccatt caggaagacc gccacgagct
     4981 gggcaatgat attactccgc gctttaaggt ggagtatgtc gacggctcaa aaggcacgcc
     5041 aaccagctac atcgccacct acatcggaaa gaacctggac agccgcgccg tggatggcat
     5101 cgacccgaaa acgggcaagc cacgcgttga ccacgaaacc ggaaaatcaa tggccgagag
     5161 cgtggaacgc gccatcggct gggcacgcct tcaccgggtc cgccagttcc agttctttgg
     5221 catcccctcc cgtcaggtat ggcgtgaact ccgccgcctt gccagccaga tggcacgcaa
     5281 cccggaaggc ccacaacggc tgaaggatga tgcaatggat gcggtactcg ctgccgctga
     5341 tgccgggtgt tttgccacct acattgagaa acagggcggc gtacttgttc cacgcaaaga
     5401 ctacctgatt cgcaccgcct acgacctcgc agatgagctg aacgattacg gcgaacagag
     5461 cgtacagatt tacgggatct ggtcaccact catcggggaa tcctcccgtg tgtgcacgca
     5521 cccggataac tggaagctgg taagacgtaa accggaagcg gaagacagca cccgcgaaaa
     5581 tggttttgac cttcagggcg gccctgccgc cccttggact cgtggcaata actgtccccg
     5641 tgtacaggaa acggacaaca acgggacaga acagccggaa gaaaggccag caccgtggcc
     5701 gcagcttcct gacggcgttg acgtgaatga atggatgcgc tcactgaaac ggcacgaacg
     5761 ccgggcgctg atgcgttcgc tgcgtgacaa acaggcaaaa aacagcagtg atgaaatgca
     5821 gaactggaca cagagccgca aacagccacg gcctttgcct gataatcacg agttactcgc
     5881 taaagaatgg cgggagtctg ctgaatctct cggcctgcat atcggtgaac agcagatgca
     5941 gcacctgtta cggggcggca gtctgtacgt tgacggcagc atcattgcac cgcagggatt
     6001 tgaaattgta cgcaaaccag atacccgccc ggacagccga atcacgcaac tctggcagcg
     6061 cctgagccgt aatcacggcg taagcagcac ggagatccgc cataacccgg tcgccagcta
     6121 tctggaacag ctgggggcat cagaccccga agccgccgca cacctggcat ccacacttca
     6181 gcaggaccag aacaccatga aaacccccgt taccgtgctt tctgacatgc tgcgcgccat
     6241 ccgtgacgca gtgcacgcac agagaatcag tgaaaccact gaacgcgccc accgcaaagc
     6301 agacctgctg cggggtagcc tgaccagtgg aaacaaaaaa cagacagaaa cgggactcac
     6361 aaatcccgta aatgagcaaa aaacgtgccg cgatatatga agcgcgcaca aaacaggcaa
     6421 aagcgggatt taaaaatcct gtaaccgatt aattaatcaa cacaaggaaa agcgacatga
     6481 aaatttgtat cgacgacggc tccaccaaca tcaagctggc atggactgag aacggcgaac
     6541 gccgcaacgc catcagcccg aacagcttca agtcggaatg gtctgcgccg ttcggtggca
     6601 cgcagcccgc gaactacatg cttgatggcg tgcgctatgg ttttgatccg gtcagcgatc
     6661 gctttgtcca gacgaccgac acgcaatacc agtacagcga tgtgaatata attgccattc
     6721 atcacgcgct ggtcaaatca ggcatcacac cacaggaagt ggatgtggtt gtcaccctgc
     6781 cactgagcga gtatttcgat acaaacgcac agccggacat ggccaacatc aaccgcaaaa
     6841 aagcgaacgt catgcgcccg gtggagtacc agaacggcga agcattcact atccgtaacg
     6901 tacgggttat gcctgaatcc attccggctg gctttaaggc actggctgac atgagtccgt
     6961 ttgaatccct gctgattgtg gatttgggcg gaaccactct ggatgtggca aaggttcagg
     7021 ggcaactggc aggtatcagc caggtgtttt gcgatccaca cgtgggcgtt tctctgatgg
     7081 ccgatgccgt actgtcggtg atggccacta acggtatgcg caccagtcac cacatcgcca
     7141 ataccattat cgaacatcgc catgatgaag cctggctgcg ccagcacatc cacaatgacg
     7201 cgcattacgc cagcctgatg gcggttattc gtgaaaagga agaaacactg aaacaacgcg
     7261 tgatccgcgc gctggcgggt ttttcgggtt acgggcgggt gatggttgtc ggtggagggg
     7321 cggagattgt ggcacccgct atccgcgaag cctgcggagt taatgcgact ttcatcgcgg
     7381 acggggtgcc acagtttgct ctggttaatg ggctgtacgc aatggacaag gagtaaacca
     7441 atgacgactc caaccagacg gataagtttc tatctgaagc ccaccgccgt caagaacgaa
     7501 ggagaagcat gcgcctggct ggacagcctt acaccagaag cccgcaaaag cggccaacgc
     7561 gtggcttttc tggccgggct ggcacttctg aaaatgaatc cggcagaggc ttatcgactg
     7621 gctgcatggg ctggtgatga agcgttatca gtgacacaaa ccaggacaga acgccccgca
     7681 tcacagccag tatcaaccgc acagataacc agtcagatgg ccggaaatat ccgggcgtta
     7741 tttcctgaat aacacaacat cagggcgagt tcgccctgct ctccacctca gaacataaac
     7801 aaggagaacg acttaatgag cgaaatcaac tatcaggcat tacgtgaacg ttattcacct
     7861 gtgccagtac cgaaatgccc tatttgcggc gaggaaatgt caattcagcg aatatctgga
     7921 gcacaggttg tttatgcctg ctccggttat ggtgatgatg gagatttcaa aattggccga
     7981 actcttgccg acgaacatta tgaaaaatca cgcgtaacag tgttggatgt cggggatcct
     8041 gaagtattgg cgctacttga ttggctggaa accaaagaca accgaatcgc tgaactggaa
     8101 aaaatcgcca ctgactatgc acttaaattc cagaaagcac aggacgcatt aaagtatgcc
     8161 gctttgctgc atagcaggac ggcgcaacaa acaaataatt ttgcagtatc gcttccggac
     8221 ataagcgaat atttcattaa tgacgtattt cagcccttgc gatacgagcg ggatgttgaa
     8281 agagccatca taaaggctgg cggaaaagca ttgtggcagg agaaacacga ggacagaacg
     8341 catcagtcct gcgatgtaaa tcgtggatgg tttagcccac tgacgacaga taaaaataac
     8401 acctgatccc cctcaaacca gggcgataat cgcgcatcgc cctgctgcac aatagtgcac
     8461 aagtttgcac aatttttttg aacgactttt tacccttccg gcccgcatgg cggctggatc
     8521 cgtcaaggat ccgtgcgtgc acaaaaaaac gcgctttttc tgcgcgcagg tgacggggga
     8581 acagcccgcg tttcaggggg taaatagcat cccctgaacg atgtcgcagc gacacaacag
     8641 aatggctgta tttctcacgc tgagcgtgaa aaagacgtga gggcttttga tttgatgggt
     8701 ggcagataag gccgtcaaaa tcgcactgag gcggcgagaa catgcagtca acgcggtggg
     8761 attgcgtaag agtctgaccg ccgatgatga caataagcag aaaagcgtcg tgaaattatc
     8821 tgattgatac aggagctgga gagtcggggc ataaattttt tatgccccgg cgaagcagca
     8881 gacaagcgaa gcgcgtcagg atgtgggctg ggtatctagc agtgcgtaag ggttaaagcg
     8941 gatcacctct tcgccaagcc agtcattgat gtgcttcatg gcctccataa cgggcatcag
     9001 ctcgttaatt gcgtaaaccc gcgcggcctt ctccacatca ccaaacgcac ttttttcgcc
     9061 cggcatcgcc cccatcagtt gcggcggaac gcggtgcgca gccagcacat catcacggga
     9121 tgccgcctta acattcatga actcatcctt tgcagtgatc tgctggaacg gcaaaatttg
     9181 cacaccttct ttgcccccgt tgggcgcatg gatgagtacg tttttaaacg caccaccacc
     9241 acgcgcgccc tgtaacgttt ctttcaggga gtccatgctt tcgcggttta cctgcgctgc
     9301 accgatgtag atgatgcacc cggcgtggga tccgttgtcg tagtacagtt ttctgaacat
     9361 gtccgccgaa tgagaaaggc tggccgagag taatgcgcca agatattccg gcatgccgta
     9421 gatttcctgg ttaatatccg gattcatcag gtggcacact ttgccagggc gaaactggaa
     9481 cgcgtccttg ccatcctgca cataccacca tgattcaaga tcgcttccgc gtcgcatgta
     9541 tttcgccagg gcgtgccgta atttaagcgg ttcgccgagc atattacttc taagctcaag
     9601 gaatgcgtta ccgaacacaa accagtccag cgccagcgcc gagaaatcct gccgggaaag
     9661 cagcgggtgc gggatgtagc aaccgagtaa tacattgcgc ttaaagtaaa gcgcagactg
     9721 atgccaggac gtttgccggg ctgctcttgc cagaccgtac cagtccaccg gggtttcata
     9781 ccaccgtccg ttatcagcac agtacatatt gtccagcagg tcatgcccgg tcaggcgata
     9841 aggaccatca aatgtgaatg cactgagcga tgattctttc ctgagcgcat cagcgagatc
     9901 aatgcgtgaa ctcatgcgca cttttttatt ttttctgctc atcagaactc cataaccgtg
     9961 aaacgctcgt tttctccttc gccgccaatc ggttcgttaa tgacagcaag catggttgcc
    10021 cacgcaaggt cgccgtggct gatccccctc gcgcggtccg tttcgtaagt gataaagccg
    10081 cccggtgttt tcaccttacg cacggcgtta aaggccgcga ccagctcgcg ttcggcgcga
    10141 tcgtattccc accgcccggc acgcattatt tgcagcattt tcagtaccag cgaccgtttt
    10201 gatgacagcg tgaaggtgta cggaatagca gcagggaaaa accgtttcac tatctgataa
    10261 acagcctccc cgttcccgcc cgtcacatca atgccgatgt gttccacgtt gtagcgatac
    10321 gtgaactctt caatgactct ggcctgttct tcaaactcca gcccctgaac gcgtcgcgtc
    10381 tccaccgttc gaaaacggcc accaggaaca gccggaggaa ccaccacgga cacagcgccg
    10441 ctgtcgccgt tgccactact gccgtttgcg tcatacccaa tccataccgg acgattcccc
    10501 atcgggcggg gagcaaaagg tttccagtct ttccagtcgt cgtatccgtc aacaccgcag
    10561 ccaatcagga tattcaggtt aaatgccgat tccccttcgc ggacaaactc acacatatag
    10621 agattgagga actcgtcttc ggtgttttca tcacgaattt cgtcgatatc ggtgtgtttc
    10681 cagccgtgat tgaccacatc ttccagcgtg acaatttgcc gccacgtccg gtcagggcag
    10741 ataagcccgt tatgcagcgt tttccagtcc acagaaaaac gctggcgttt atgcgtggcc
    10801 tttttctcgt tccagcggtc gccgttccag taggcgtatg cctcgtgcgt ttcggtggat
    10861 ggcgtggaga agtaggtgcg ccgcagtccg ctgagggttg ccatagcgcc agccaccttg
    10921 cgcagttcag caaagcgact gacccagaaa aattcatcaa aataaaaatt gcccgtatag
    10981 gactgtgccg acgcagcaga agtgccgaga aaatgcagct ctgcgccgtt ggagaggatg
    11041 attttatcgc cccctttcag ctccacatca acttcagccg cggccttctg aataatgctt
    11101 ttaaactgga acgcctgacg acgcgacgca gacaaaaaaa tctggttacg ctggtaaggt
    11161 tgcgccacat cgtcacgcag cgccatcagc agagcttcct gtgcaaaata ccaggtcgcc
    11221 ccaatctgtc gggatttcag gatcatcctg ttacgtatcc cggcttccct gcaaagggtc
    11281 agggagtcaa accagccccg ctgatgccac tccagcctgc tgatgatttt ttcccgcagt
    11341 gcggcaatct gttccggcgt gaaatgattt ttgagttttt tcgcccggcc tttctttcct
    11401 gtgaccgtcg catccggctg gccatcatgc agttttttaa gctgccgggt cagcaggtct
    11461 atttccttaa agtcaccgcc tgttttattc tgtttttcag taagctggat gaggcgcgca
    11521 tcgatggact gcgtgacacg ctgcacgggt ggcgtttcat cccactggtc gcgttttttc
    11581 cacgcataaa tcgtgttcgg gtttattccc atcagacgtg atatttctgc gggcggataa
    11641 ccctgccagt aaagttgccg cgcacgctgg cgcacaaaag cgtcctgaat cattgctccc
    11701 cctgagtaat tacaggaaga ttacccgcgc gcgaaactgt tctccttaac cccctgttct
    11761 ggccgttttc ttacaacaaa atccctttgt atcagcctgt tacgctttgc catcatgact
    11821 gaagaaccag tcagaggggc aaaaactatg gctaatgaaa aaaagacatc ccgcaaaaag
    11881 tttcgcgtgg ctgtctccgg atcaactgtt gatggccgcg aaatcagccc ggtacatctg
    11941 cgtgaagccg ccgagaactt caacccggat gtttacgctg cccgcgtgaa cgttgagcac
    12001 tatctctcgc catgcccgtc aagcgaattt tccgcaatgg gcgatgtcac cgcgctgagt
    12061 acggaagata ttacggaagg cccgctggcc ggacgtactg cgctgtatgc agaaatcgaa
    12121 ccgaccgagc gcatgaagca gcttatcgct gacggcaaga aaatctattc cagtatcgaa
    12181 ctgcacccgc agttctccgt taacgggcgt gcctatctgg tcgggctggc gatgaccgac
    12241 accccggcaa gcctgggcac tgagcgcctg aaattcacgg cacagcagcg tcaggcggtg
    12301 atgacgttca acagtatcca gggtgaagca ccgcttatct ccgaagccat cgagtctgaa
    12361 atcatcgaaa tggcagaaca acgccaggaa gaaggcaccc agtggtttaa ccgcgtaatg
    12421 gggattattg gtcgtggccg caaagcggat gacgccagtt tctcccgtat tcaggaagcg
    12481 gtggaaggcg ttgcaacatc acaggtcgac attatcgacc gttttaatgt gctggaaacc
    12541 cgccatcagc aggaccgcca gaaaatcacg tcactgacca cagagctgac agcactgaag
    12601 gaaaaactgc gcacgcagga cggcgatccg cagaaccgct tcaccgcaac gggcgcagcc
    12661 tccgaccagc tggctgactt ctgataagac aaaggagcaa attttttatg aatctggtga
    12721 tgtcagatat tacccgcaac aagctgggtt gctatatggc gcagcaggcg tcgcttaaca
    12781 atatcccggt atctgcactg gtatcgcgat ttaccgtgga acccgcggtg cagcagcgtt
    12841 ttgaaaacgc ctcaaaggaa agtaccgaat ttacgaaaag aattaacgtg atcggcgtga
    12901 ccgaccagaa aggcgaaaaa atcctcccgg acaccaccgg gccaattgcg cgcacgaata
    12961 gcagttatga cggcatcaaa cgccgtaacc cgaataacgt gatcgatatg aagtctcgtc
    13021 agtaccagtg cgaacaggtg aactacgata cctttatttc gtacccacag cttgatacct
    13081 gggcggccca cagcgatttt cagtcccgta tcagtacaca gatcgcccgg caggtagcgc
    13141 ttgatcgcat catgattggc tttaacggca catcccacgc ctacgagtct gattttcaca
    13201 ccaacaagct gcttcaggac gttaacgtgg gctggctgga gcacatcaga accgatgcca
    13261 gcgagcgcgt aatgaatgac gtgacgctga cctcccgcaa catggacaac actgtggcgc
    13321 acgcgggtaa gtatgcgaat gccgatgctc tggtacagga tgcgcgctca tccctgctgg
    13381 atgaatggca caaggaagct gacgacctcg tggtgattat ggggcgcaac ctgtttaact
    13441 cgctgcgtct gcccgtgctg aacagcatca gcggccagaa tcccaatgcg gaattacttg
    13501 ccgggcagct catcctgtca tcgcgcacca ttggcgggct gggcgtgttc cttgcgccgt
    13561 tcttcccgga tgcaacgatg cttatcacct cgttcaacaa cctgtcgatt tactggcaga
    13621 aaggttcaat gcgtcgtctg atgaaagacg agccggaata caaccgcatc gccacctacc
    13681 agtccatcaa tgacgcttat gtcgttgaag actatggcaa gtgcgcgatg gtcactggcc
    13741 tgaagttcgc cgacagctaa tcaactcacg gcgggcatca tgcccgcctg taacggagag
    13801 aaaaaatgat tactcctgca caacaacact ggcagaacgt gatggcacag cgcgcaggcc
    13861 gggcgaatga aggtgtggac cacgccgcgt gtaccgcgca tgaagaagtg ctgtatcgtc
    13921 tgcgtctggc acaggccagg ctaaaggcca tacaggccag aagcgcgaaa gccgccatca
    13981 aaaaagagtt gttgccggac ttttccggct ggattgaggg aacgctggag gctgacggcg
    14041 ggcaacagga cgaagtaatt gccacgctga tggtgtgggc gattgactgc ggcgatcttc
    14101 cgctggcgct gcgtattggt gcatatgtgg tccgtcacaa cctcatcatg ccggataact
    14161 ttggccgtac tgctgccacg gtactgaccg aagaaatctg caacccggta ctgacgcagg
    14221 ctgggacgga tgccgacgcg gatttatccg cctttatcga accactggac acactttggg
    14281 aaattgtcgc caaccaggac attccggacg aagtgcgcgc caaattatgc aaggcgtgtg
    14341 cctttgcccg tcgtggcctg accgatgcag acaacatggc ctcatcactg aagctgctgc
    14401 gcgaagcgat gcacctgaac ccgaacgcag gtgtgaaacg cgagattgca accctttccc
    14461 gcgccctgaa aaaagccgat tccgcagccg aaccagaaga cgccagcgca cagcaggcgc
    14521 aggacgaaag cagcaaaaat aaaaagacaa cgcggaagcc tgcaacacga aaaaccaccg
    14581 cgacgcagaa ggcaaagcgc ggttaacgac tgaccccgtc agcgggcggc gtgcgcggtg
    14641 ttccggtttg actccgtgac cgtttacacc gcgcacccac cgcccgattt tttcaggagt
    14701 gaaccccatg agtatggttg ccagaactaa ccccggcccc gcagaggacg acatcaccga
    14761 taccgatgat ggcgacactc gcatttcagc gggtgcattc tggccggata ttgtgctgcg
    14821 tgagctgcgt ctggcggtac gactgccggg ccgcgtgacc acctcccgcc tgctgcatac
    14881 cgccaccggg gccatagcac acgttacccg cgagctggaa gcatggcagc aggaacaaca
    14941 ggcggctggc tatcagacgc tggccgatgt tccggcccct gtaattaacg gagaaagcgt
    15001 caatctctgg cactggcgca atgctgttta taccgccaca cgcgccctga ttctggagcg
    15061 ttaccgcgat gcggacacaa cggacaaggg cgaccgccgg gcggacgcac tggatataca
    15121 gacatcggat ttgtggcgcg atgtgagctg ggccatctct gacattctgg gacgaccgcg
    15181 aatgtttgcg gagctgtgct gatgaaagtg aaggcactgg aaggcgacac cgtggattcg
    15241 ctctgtttcc ggtactacgg cacgacacgg ggcgtcaccg aaaaggtgct ggatgccaac
    15301 cccggactct gtcagcaggt atttctggac gccgggcagg aagtggagat gccggagccg
    15361 gagaagaaga aacgagaaat gattcagttg tggggggagt agcagtgagc accattcaaa
    15421 cagggatcac agagcaggtt attgcgtggc tctttgacca cctgccaacg gtgtatgcag
    15481 taggcgcggc tgtcagcatt tccgcgctga tgagtcttta tgacggacga acactggttc
    15541 agaccgtaac gggatcgctg gcgtgcggcg ttcttgccat ggccgtggcc gggtcgctgc
    15601 gcttcttcgg gatccctgag gatgcagtga cgttttttgg tgcctcaatc ggttttatgg
    15661 gcgcagagaa agcacgcgac aaggttattg caatatttga tcgcagggtg aaggagagga
    15721 acgaatgagc aacacattta aattcagcag ccgaagcgaa aagaatttgc agggtgtaaa
    15781 tcctgatctg gtgaaagtga cccgacgggc gctggaaatc tcggaagtgg attttggtat
    15841 caccgaagga ttgcgcagtc gttatcgtca gaagcagctc gtgaccacag gcaagagcca
    15901 gaccatgaac agtcgccatc tcacagggca tgccgtggat gttgtggctt atgttggcag
    15961 ccaggtgtca tgggaatggc cgctgtacga aaaaatcgca gcagcattca gacaggccag
    16021 ccgggaactg aatattccgg tggaatgggg cggcgactgg aagaccctga aagacggacc
    16081 gcattttcag ttaccacacg gagcctatcc ggcatgaagc tctggcccac gcttggcgtc
    16141 gctttccttc tgattgccgc atggggaaca tccatgcgtc tgtcgtggtc gctgggccgg
    16201 gagaacgcca gaaacgaagc gcaggccagc accctgaaaa gtaccgtcga cacactgaat
    16261 atcatcagcg ccggggtaca ggatatgcag caggtgctgg ctcaactccg cgcggacaat
    16321 cagcaacgca atcaggacgg agaggtaaga cgtgaacagc tacgcaacga tattgcaaaa
    16381 gatgaatgcg cccacgcttt gcctgacgct cgttttactg acaggttgcg caggcacgca
    16441 gaacgcgcca cggccagcgc cgtcagtccg gcttataccg cagacgctga ccatgccggt
    16501 aacgcctccc cccttccctg accaacccac atggggaaac ctcggaatat ggggcgaccg
    16561 ccttctggat gcactggaaa cctgtaacgc ggataaacgg gccattgctg aactggataa
    16621 gagaatagcc gaactgacac accagacggg agtaacacaa tgaccagtaa gaactttgca
    16681 ctgattacag ccatgacaca ggctgaactg actcaaaagg tgaatgaaca tcttgcgaaa
    16741 gggtggcatc ttcaggggga gacgcgggtt gcctacgaac ccggcacccc gtggtatcta
    16801 atgcaggcaa tggtggccga tggcactaca gacatctcac ctgattcccc ccagcacggc
    16861 agcgtgccgg agtggtatta cgtggtggta cttgctggtc aatccaatgc catgtcatat
    16921 ggtgagggaa tgccgctgcc ggattcttac gatgcgcccc acccacgcat taagcaactg
    16981 gcccgtcgca acacagtgac tcccggtggt aaagcatgcg catttaacga catcattccg
    17041 gcagatcact gcctgcatga tgttcaggat atgagcgtac tgaatcaccc gaatgcagac
    17101 ctgagcaaag ggcagtatgg ctgtgtcgga cagggcttgc atattgccaa acgcctgttg
    17161 ccttacattc cacagaatgc cgggatttta ctggttccat gctgtcgtgg tggttcggca
    17221 ttcacccagg gcgcggaggg gacattcagc gagtccacag gagccagtca ggattcggct
    17281 cgctggggtg tgggtaaacc tttatatcag gacctgattt tgcgcacgaa ggccgcattg
    17341 cagaaaaacc caaaaaacat gctgctggcc gtatgctgga tgcagggcga atttgacatg
    17401 agcgccgcta cgtactcaca gcaacctccg ctgtttgcgg ccatgctgaa acagtttcgt
    17461 gcggacatta ccgagtttaa cacgcagtgt catggaggca gagcggcaag tgtgccatgg
    17521 atttgtggtg acacgacgta ttactggaaa aacacctacg gcacgcagta cgacaccatt
    17581 tacggggcgt acaaaaacag ggagagcgac aacgttttct ttgtgccatt cctgaccgat
    17641 ggtagtggca acaatacctc caccaacgca ccaacggaag atccggatgc tgcaagtgag
    17701 ggatattacg gttcggcatc ccgaacgaac aaaaactggg tatcatcaaa tcgccagacg
    17761 catttcagtt catgggcgcg caggagcatt attccggatc gtatggcaac cgctattctg
    17821 aacgtagccg gtcgcacctt
//
LOCUS       DAZKGS010000086        17826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKGS010000086 DAZKGS010000000
VERSION     DAZKGS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            82..423
                     /gene="symE"
                     /locus_tag="ACHOQY_002948"
     CDS             82..423
                     /gene="symE"
                     /locus_tag="ACHOQY_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP9679009.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(470..1633)
                     /locus_tag="ACHOQY_002949"
     CDS             complement(470..1633)
                     /locus_tag="ACHOQY_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFP9679010.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1681..2563)
                     /locus_tag="ACHOQY_002950"
                     /pseudo
     CDS             complement(1681..2563)
                     /locus_tag="ACHOQY_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2660..2824)
                     /gene="yjiS"
                     /locus_tag="ACHOQY_002951"
     CDS             complement(2660..2824)
                     /gene="yjiS"
                     /locus_tag="ACHOQY_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP9679011.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3001..4413
                     /gene="yjiR"
                     /locus_tag="ACHOQY_002952"
     CDS             3001..4413
                     /gene="yjiR"
                     /locus_tag="ACHOQY_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9679012.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(4655..5599)
                     /locus_tag="ACHOQY_002953"
     CDS             complement(4655..5599)
                     /locus_tag="ACHOQY_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9679013.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            6066..7298
                     /gene="mdtM"
                     /locus_tag="ACHOQY_002954"
     CDS             6066..7298
                     /gene="mdtM"
                     /locus_tag="ACHOQY_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP9679014.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            7339..8619
                     /gene="yjiN"
                     /locus_tag="ACHOQY_002955"
     CDS             7339..8619
                     /gene="yjiN"
                     /locus_tag="ACHOQY_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9679015.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            8735..9886
                     /gene="yjiM"
                     /locus_tag="ACHOQY_002956"
     CDS             8735..9886
                     /gene="yjiM"
                     /locus_tag="ACHOQY_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9679016.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9896..10663
                     /gene="yjiL"
                     /locus_tag="ACHOQY_002957"
     CDS             9896..10663
                     /gene="yjiL"
                     /locus_tag="ACHOQY_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9679017.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            10660..10917
                     /gene="ytiA"
                     /locus_tag="ACHOQY_002958"
     CDS             10660..10917
                     /gene="ytiA"
                     /locus_tag="ACHOQY_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9679018.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            10982..11842
                     /gene="yjiK"
                     /locus_tag="ACHOQY_002959"
                     /pseudo
     CDS             10982..11842
                     /gene="yjiK"
                     /locus_tag="ACHOQY_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            11910..13088
                     /gene="yjiJ"
                     /locus_tag="ACHOQY_002960"
     CDS             11910..13088
                     /gene="yjiJ"
                     /locus_tag="ACHOQY_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679019.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13101..13655)
                     /gene="kptA"
                     /locus_tag="ACHOQY_002961"
     CDS             complement(13101..13655)
                     /gene="kptA"
                     /locus_tag="ACHOQY_002961"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP9679020.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            13905..14588
                     /gene="yjiH"
                     /locus_tag="ACHOQY_002962"
     CDS             13905..14588
                     /gene="yjiH"
                     /locus_tag="ACHOQY_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP9679021.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14585..15046
                     /gene="yjiG"
                     /locus_tag="ACHOQY_002963"
     CDS             14585..15046
                     /gene="yjiG"
                     /locus_tag="ACHOQY_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9679022.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15059..16231
                     /gene="iadA"
                     /locus_tag="ACHOQY_002964"
     CDS             15059..16231
                     /gene="iadA"
                     /locus_tag="ACHOQY_002964"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9679023.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16296..17208
                     /gene="hypT"
                     /locus_tag="ACHOQY_002965"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             16296..17208
                     /gene="hypT"
                     /locus_tag="ACHOQY_002965"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(17201..17593)
                     /gene="iraD"
                     /locus_tag="ACHOQY_002966"
     CDS             complement(17201..17593)
                     /gene="iraD"
                     /locus_tag="ACHOQY_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9679024.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17590..17673)
                     /gene="idlP"
                     /locus_tag="ACHOQY_002967"
     CDS             complement(17590..17673)
                     /gene="idlP"
                     /locus_tag="ACHOQY_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP9679025.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         4161 a         4518 c         4473 g         4674 t
ORIGIN      
        1 ccaggtattt agcgcggtgc ggatgtgcgc caacacaccc gcaccgctaa tcacaatcac
       61 tattatcgga gaatagcagt tatgactgac acgcattcta ttgcacaacc gttcgaagca
      121 gaagtctccc cggcaaataa ccgtcaatta accgtgagtt atgcgagtcg ctacccggat
      181 tacagccgta ttcccgccat caccctgaaa ggtcagtggc tggaagccgc cggttttgcc
      241 accggcacgg cggtagatgt caaagtaatg gaaggctgta ttgtcctcac cgcccaacca
      301 cccaccgccg aagagagcga actgatgcag tcgctgcgcc aggtgtgcaa gctgtcggcg
      361 cgtaaacaaa agcaggtgca ggattttatt ggggtaatta ccggtaaaca gaaagtcgcc
      421 tgataaattg acccggtagc gccagtgcta ccgggtatta ttcgccgcgt tataccaacg
      481 cgctctccat ctgtttttca aatgcctgac gaattaatcc cataatgtaa ggaagttgtg
      541 cgaggtcgct gaaaccaatt tctacatcgc cattacccca gcgcccaaca tttgtcacat
      601 ctttggcaat acctttcgga tcgaccagtt catgaaactg catattaagc gacaaccgca
      661 gtcggctttt ttgcggtaca acatcaacaa aattcgtttc agctttaaac gcaatataga
      721 gcttcaatac ttcctgcgtg atccctgcgt ccaggcgcat aacttcatcg cgaagatgat
      781 cgaataacac ccggctatgc gaaccatcag caagaaaagg ataatcactc aggctgtaac
      841 tggttttgtt ttcaggctgc ccccgataaa cagctaaaac ctcttcagaa agagaaggcg
      901 cttgccagac ctgaaccgcg agatcggcca gacgatcggc acgggcgcga atggcggctt
      961 catcccattt ttcgcactgc ccaagaccga tattcaaata gagcgggcta tgtttaaagc
     1021 cgtcttcaat atcgcgtttt tccgcgaaag gtctgtcact gtagcgggag ttatagcgcg
     1081 ttaaagtgag attccccaac gtatgtaaca gttctttatg cacacgctgc cagtcgcttc
     1141 ccagctcttc acgccattta gccgacagat tttcgttctg cggcataatg tgctcaatcg
     1201 taaactcttc cacgcgctct ctgcgtttat cgttttccag tcttcgtaac cagtagctgc
     1261 ggctgcggaa gttatagaga tcgcgaactt ttaattcccg tttaaattca tcatcgttgg
     1321 ggaaacgacg atatgaaggc aggttcataa aatgtacctg aatactttcc agatattttt
     1381 ctttattaat gaccttataa aaagtggcaa acgtcttgtt caaagaattc gtcggaattg
     1441 cacatactgc acggcggaaa acataagatt caattaaacg aattatgctc aggaaatctt
     1501 cgtgagacaa atcgtcattt ttatagtcat gataaagcgc cagtaagaaa ggatacgcca
     1561 catcaacctt taactcgcgc aaatcctgaa aagccgtagc aagcgatttg tcactttctt
     1621 ttcccaatgc cattgcacag taatactccg cgtagatatg aatatcttca accagccgat
     1681 ctacgccttt ttcagcaaca ctctggctgc gggcatggag tttaaatgcc tcatagactt
     1741 catctgtccg agggatctcc cccgttttta ccgtcagata atgacgcata aaactgtcaa
     1801 aatattcgct gtaaccttgc tgaccaaagg cgacttccat tggacgccag tgatcttcat
     1861 acaaccgggt ttgatgctct ggttcgaggc ccatcagaat aaaattgcgc accagatcgg
     1921 cctgacttaa cgccttaccg gtggagttca tactttcaaa aatcagttgc ggattatcct
     1981 gaccacgatt aagcgccaca tcgacaatta ataactttgc taacccacga cataagggga
     2041 tcaagtcatc accgagtttg gcaatttgtt catcaaagaa agtaaagttt tccattattc
     2101 gatgggaata gttttctggc attggtcttt gttttattaa cgccagtaaa ctgtctttat
     2161 cagtctcggt cagtagtaat ttaaaacctt tctcgccgga ctcataagga ttgagcaaat
     2221 aataattacg caatttcatt gctgaaaagc catcaaaaac ttcgtcttcg ccaagatggc
     2281 gcgataaagc ctcaatcagc aacattgcgg tcgtcagccg ttgttgacca tcaatgacca
     2341 gtaacggaga aataccagaa acctgataca acccctgctc aatataaaca accgaaccga
     2401 taaaatgcgc tgatatatcg tcacgctttc cggcacgaat gatgtcgtcc caaagttgcc
     2461 gacattgttg ttctgtccat gaataagtcc gttgataaat gggaataaca aactgctgcg
     2521 aacgttttaa aaaatcgagt aggcgggctt ctgtagcttt cattctgacg tctttccgtg
     2581 aagcgatgat ttcgcaataa taaccacttc cttttggagg ttatagagaa atcctgttta
     2641 tccgcgatga attgccccct cactccacat cctccctgcg taacccaata tctctcaacc
     2701 gctcatcact catctgctgt aacactcgtc gggtctgcat ttgccgccgc caacgcctca
     2761 ccgcctgcca gagttgcaca agtctgataa acggcgtttt ggctttgttt tcgtgaaatt
     2821 ccatctgctg cctcctcatc tttcagaggc ttcatcatcc accttctggc agttcacaat
     2881 acagatgcaa atattagttt tatttaacat acagacaggt taaaacagca tctgcatccg
     2941 gttttttagc ctcatctgta ccggtttttc tttctgtatg gtccaaataa ggaatacagc
     3001 atgacgcgtt atcaacatct ggcgactcta cttgccgagc ggattgagca agggctgtat
     3061 cgtcatgggg agaaattgcc gtcggtgcgt agcttaagtc gggagcacgg cgtcagcatc
     3121 agcaccgtgc agcaggcgta tcagacgctg gagacgatga agctcataac cccacagccg
     3181 cgttcgggtt attttgtcgc acaacgtaaa gcccagccgc cagtgccgcc gatgacgcgt
     3241 ccggtgcagc gcccggtgga aattacccag tgggatcagg tgctggatat gctggtggcg
     3301 catagcgaca gttccattgt tccgttaagc aaaagcacgc cggatgtcga aacgcccagc
     3361 ctgaaaccgc tctggcggga gctaagccgg gtggtgcagc ataatctgca aaccgtgctc
     3421 ggttatgact tgttagccgg tcagcgggta ttgagagagc agattgcccg cctgatgctc
     3481 gacagcggct cggtggtcac cgccgatgac atcattatca ccagcggctg ccataactcg
     3541 atgtcgctgg cgttaatggc ggtgtgtaaa ccgggcgata ttgtcgcggt cgaatctccc
     3601 tgttattacg gttcgatgca gatgctgcgc ggcatgggcg tgaaagtgat tgaaatccca
     3661 accgatccag aaacaggcat cagcgttgaa gcgctggaac tggcgctgga acagtggccg
     3721 attaaaggca tcattctggt gccaaactgt aataatccgc tgggatttat tatgccggac
     3781 gcacgcaaac gggccgttct ctctctcgct cagcgtcatg atattgtgat ttttgaagat
     3841 gatgtctacg gcgaactggc aacggagtat ccgcgcccgc ggaccatcca ttcatgggat
     3901 atcgacgggc gagtgctgtt gtgcagctcg ttcagtaaaa gtattgctcc aggcctgcgc
     3961 gtgggttggg gcgcaccggg gcgttatcac gataaactga tgcatatgaa atacgccatc
     4021 agcagcttta atgtgccgtc cacgcaaatg gcggcggcaa cgtttgtgct ggaaggtcac
     4081 tatcatcgcc atatccggcg gatgcggcag aactatcagc gcaatttggc gctttatacc
     4141 tgctggatac gggaatattt tccctgcgaa atctgtatta cgcgcccgaa aggcggattt
     4201 ttactgtgga tagaattgcc tgaacaggtc gatatggtct gcgttgcgcg gcagctgtgc
     4261 cgcatgaata tccaggtggc agcaggctcg attttctcgg cttccggcaa ataccgtaat
     4321 tgtctacgca tcaactgcgc tttgccgctc agcgaaacct atcgcgaagc actaaagcaa
     4381 attggcgagg ccgtgtatcg ggcaatggaa taaattcgaa ttagtggcga agcacctgat
     4441 ttcctctcac gaatcgcctg taactcgttg tatctttgca gggtcatcgc cacgttccag
     4501 catggcttag gcaaagtgtg cttcgttcat accggatgcg gcgtgaacgc cttatccggc
     4561 ctacaaaccg cgcaaattca atagattgca gaggaaatgt aggtctgata agcgtagcgc
     4621 atcaggcagc tttgcgtttg tcatccgtct ctgattagta ttcgatccat ggatcgcgct
     4681 tgatttcagg caagggctgc tctaatgcct gcatttgctc tgtcgaaagt ccggtaatct
     4741 tttgtatcca ttcaggatct gccccattct gcaacaacaa tctaaggagg cgttgttccc
     4801 cttcttcttt ccccttttcc attcccaaca gccatccatc attataaatt cgctctgcaa
     4861 tcgtcattat tcgctccctg tgttgtggca ttcgacgggt aagttcgctg ataaacttct
     4921 taaaacgcgc ttcatcgcca gtcagtaaaa tgtaatttaa cagcgcagtt atctggctgt
     4981 cattagcaca ctctgtaacc agtaatacta ccagttggtc gataagcccc atcagatcgc
     5041 gctggcgaat atgcttttgg atcaactcca acagggcgac tctgcgatgc tgcacaatct
     5101 cgtcgtctgg cacgacagtg atatccacca gcgggaacgc ggcggtataa agtttccgtg
     5161 cggtagttgg gtcggcaaat tcgtccagcc agcacaggga ccagggataa ggactacggc
     5221 taccgtgata aaatagcatc ggtataacca gcggtagcgc ccgacgttta tcatgctcga
     5281 tatggcgctg catcaccgcc atggaatagc gcatcaggcg aaaggccata tgaatatcct
     5341 cacggctctg atgttcaatc accacataaa tatagccatc gccttcgcgg gtatttaccg
     5401 accacagaat atcggagtgt agcgcccgca atttttcatc gacaaagctg gcggattcca
     5461 gttttaagct gtcgagatcg cacagttcac gtaaatcttt gggtaaatag atctccataa
     5521 aatcccgcgc ggtgtcaggg tgggtgagaa aggatttaaa taatgcgtca tgcggcgtgc
     5581 tggttgtgaa gtttgtcatg gcgatccgtc acctgttgat aagatggccc gactttagcg
     5641 actaacaaaa ccattcacac ctggcaattt cccgttcccc gaggcgcttt ccaggctatt
     5701 tttgttcctc tgcaagcgat acatttttcc ggaaacaggc tcgcgtttta catttcctgt
     5761 acccggtaga tattcccctt tccaccgcgc tcttttgatt aatcgcaaat tttaattaca
     5821 cttaaggtgt atattttcta tgcaaaccat caattcaaga ggtgtaatgt gctgatgact
     5881 atttgtaatc gttatacatt ctgacccgaa ctcagaaggt atttctctgt ctgtataagc
     5941 acaggcagtg tggttgatta catgaaattc agtacatttg tccttctcgc ctacctttcg
     6001 tcattaccga cggtattgaa tttcgttttc cccgttgggg ttctccggac aaggagttgt
     6061 ttgttatgcc acgttttttt gcccgccatg ccgccacgct gtttttcccg atggcgttga
     6121 ttttgtatga ttttgccgcg tacctgtcga cggacctgat tcagccgggg atcattaatg
     6181 tggtccgtga ttttaatgcc gatgtcagtc tggccccggc agccgtcagt ctctatctcg
     6241 cgggcggtat ggcgttacag tggctgctgg ggccgctttc cgacaggatt ggccgcaggc
     6301 cggtgctgat taccggggcg ctaatattta cccttgcctg cgctgcgaca atgttcacaa
     6361 cgtctatgac acagtttctt atcgcgcgtg caattcaggg caccagtatc tgttttattg
     6421 ctaccgttgg ttatgtcacg gtgcaggagg cgttcggaca gacaaaaggg atcaagttga
     6481 tggcgattat cacctccatc gtactgattg cgccgattat tggcccgctt tccggcgcag
     6541 ctctgatgca ctttgtgcac tggaaagtcc tttttgccat cattgcggtt atgggtttta
     6601 tctcatttgt tggcttactg ctggcgatgc cagagacggt gaagcgcggc gcggttccat
     6661 ttagcgccaa aagcgtcttg cgcgattttc gtaatgtctt ttgcaatcgg ctgttcctct
     6721 ttggcgcagc aaccatctct ttaagctata tcccaatgat gagttgggtg gctgtctcgc
     6781 cggtgatcct gattgacgca ggcggcttaa caacttcgca gttcgcctgg acacaggttc
     6841 cggtgttcgg cgcggtgatt gtcgcgaatg ccatcgtggc gcgtttcgtt aaagatccga
     6901 ccgaaccgcg ctttatctgg cgtgccgtac ccattcaact ggtcggcctc gcactgttga
     6961 ttatcggcaa tctgctgtca ccgcacgtct ggctgtggtc ggtgctgggc accagtctgt
     7021 atgctttcgg gattggttta atcttcccga ccttattccg ctttacactt ttttccaata
     7081 acttaccgaa agggaccgtc tccgcatcgc tgaatatggt gatcctgatg gtgatgtctg
     7141 tctcggtcga aatcggccgc tggctgtggt ttaacggcgg tcgcttgccg tttcatctgt
     7201 tagccgtcgt agcgggagtt atcgtcgttt tcaccctggc ggggttgctc aatcgcgtgc
     7261 accaacatca ggctgccgag ctggcggagg agcggtgatt tttgcgcgat cctgccgtca
     7321 ggctctattc ttaacgttat gaataaactc attgaactca gacgcgccaa aatgttggcg
     7381 ctctctttac tgcttatcgc cgctgctacc tttgtcgtta cgctgttttt gccacccaat
     7441 ttttgggtga gcggcgtgaa ggcaattgct gaagcggcga tggtcggcgc gctggcggac
     7501 tggtttgcgg tggtggcgct gtttcgccgc gtgccaattc cgatcatttc tcgtcatacg
     7561 gcgattatcc cgcgtaataa agaccggatt ggcgaaaatc tcggtcagtt cgtgcaggaa
     7621 aaatttctcg atacccagtc gctggtggca ttgattcgac gtcacgaacc ggcgttgcta
     7681 attggtaact ggtttagcca gccagaaaac gcccgccgcg ttggtcagca tctgttgcag
     7741 atcatgagtg gttttcttga actgaccgat gatgcacgta ttcagcgcct gcttaagcgc
     7801 gcggtccatc gggcgattga taaggtcgat ctttccggaa ccagtgcgtt gatgctggag
     7861 agtatgacca aaaacgatcg tcatcaggtg ctgctcgata cgctgatcgc acagttgatt
     7921 gctcttctcc agcgcgataa atcgcgcaag tttatcgccc aacaaattgt tcgctggctg
     7981 gagagtgagc atccactgaa agccaaaatt ctccccaccg aatggctggg cgaacatagc
     8041 gcggagttgg tttctgacgc ggtgaattct ttgcttgatg atatcagccg cgatcgtgcg
     8101 catcagatcc gccatgcatt tgatcgcgcc acttttgccc tgatcgacaa gttgaaaaac
     8161 gatccggaaa tggcagcgcg agctgatgcc gtaaaaagct atctgaaaga ggatgaagcc
     8221 tttaaccgct atctcagtga attgtggggg gatttacggg aatggctgaa agcggatatc
     8281 aacagtgaag attctcgtgt gaaagaacgt atcgcgcggg cgggtcaatg gtttggcgaa
     8341 acgttaattg ccgatgatgc cttgcgggcg tcgttaaatg gtcatctgga acaagccgcg
     8401 caccgcgtcg cacctgagtt ttccgcattc ctgacgcgcc acatcagcga tacggtaaaa
     8461 agctgggatg cccgggatat gtcgcggcaa atagagttaa atatcggcaa agatctgcag
     8521 tttatccgtg tcaacggtac gctggttggc ggttgtattg ggctaatttt atatttgctg
     8581 tcacagctcc cggccttgtt ccccctcggc aatttttaga aatccatgaa aaactaagtg
     8641 gctaaccgcg agagagatca aataaaccac actaaaagaa tggtaataat gtggcctctt
     8701 ttcattatct cccgtggtac ggggaaggaa aatcatgtca cttgtcaccg atctacccgc
     8761 cattttcgat cagttctctg aagctcgcca gaaaggcttt ctcaccgtca tggatctcaa
     8821 ggagcgcggc attccgctgg ttggcactta ctgcactttt atgccgcaag agatcccgat
     8881 ggcagccggt gcggttgtgg tttcgctctg ttccacctct gatgaaacca ttgaagaagc
     8941 ggagaaagat ctgccgcgca acctctgccc gctgattaaa agcagctacg gcttcggtaa
     9001 aaccgataaa tgcccctact tctacttttc tgatctggtg gtcggtgaaa ccacctgcga
     9061 cggcaaaaag aaaatgtatg aatacatggc ggagtttaag cctgttcatg tgatgcagct
     9121 gccaaatagc gttaaagacg atgcctcgcg tgcgttatgg aaagccgaga tgctgcgctt
     9181 acaaaaagcg gtggaagaac gttttgggca cgaaattagc gaagatgctc tgcgcgatgc
     9241 cattgctctg aaaaaccgcg aacgtcgcgc actggctaat ttttatcatc ttgggcagtt
     9301 aaatcctccg gcgcttagcg gcagcgacat tctgaaagtg gtttacggcg caaccttccg
     9361 gttcgataaa gaggcgttga tcaatgaact ggacgcgatg accgcccgcg ttcgtcagca
     9421 gtgggaagaa ggccagcgac tggacccgcg tccacgcatt ttaatcaccg gctgcccgat
     9481 tggcggcgca gcagaaaaag tggtgcgcgc gattgaagag aatggcggct gggttgtcgg
     9541 ttatgaaaac tgcaccgggg cgaaagcgac cgagcaatgc gtggcagaaa cgggcgatgt
     9601 ctacgacgcg ctggcggata aatatctggc gattggctgc tcctgtgttt cgccgaacga
     9661 tcagcgcctg caaatgctca gccagatggt ggaagaatat caggtcgatg gcgtagttga
     9721 tgtgattttg caggcgtgcc atacctacgc ggtggaatcg ctggcgatta aacgtcatgt
     9781 gcgtcagcag cacaacattc cttatatcgc tattgaaaca gactactcca cctcagatgt
     9841 cgggcagctc agtacccgtg tcgcggcctt tattgagatg ctgtaaggag tggcagtgac
     9901 atattcgatt ggcattgatt ctggttctac cgccaccaaa gggatcttac tggcagacgg
     9961 cgtgattacg cgccgtttcc tcgttccaac accctttcgt ccggcaacag caattactga
    10021 agcctgggaa actctgcgcg aagggttaga gacaacgccg tttctgacgc tcaccggcta
    10081 tggacggcaa ctggtggatt ttgccgataa acaagtgacg gaaatctcct gtcatgggct
    10141 gggcgcacgg tttcttgcgc cagcaacgcg cgcggtaatc gacatcggtg gtcaggacag
    10201 caaagtgatt cagcttgatg atgacggtaa cctgtgcgat ttcctgatga atgacaaatg
    10261 cgcggcgggc accgggcgtt tcctggaggt gatctcgcgc acgcttggca ccagcgtcga
    10321 gcaactcgac agcattaccg aaaatgtcac gccgcacgcc atcacgagta tgtgcacagt
    10381 gtttgctgaa tcagaagcga tcagcctgcg ctcagcgggc gtcgcgccag aagcgattct
    10441 cgccggagtg attaacgcga tggcgcggag gagtgccaat ttcattgctc gtctctcctg
    10501 tgaagcgccg attctgttta ctggtggcgt tagtcattgc cagacgtttg cccggatgct
    10561 ggaatctcat ctgggaatgc cggtaaatac ccatcctgat gcgcaatttg ctggcgcaat
    10621 tggcgcggcg gtaattggtc aacgagtgag gacacgccga tgaaagagtt tttattttta
    10681 tttcactcca cagtcggcgt catacaaacc cgcaaagcgt tgcaggcagc gggcatgact
    10741 tttcgcgtca gcgatattcc ccgtgattta cgcggcggct gcgggttatg catttggctg
    10801 acctgtcccc ccggcgagga aatgcaatgg gtgatccctg ggctgactga gtcaatttat
    10861 tgtcagcagg atggtgtctg gcgctgcatt gcacattacc gggtttctcc gcgttaagga
    10921 ttcattaagg tcgctacgta tacttctcga tatcttattg atttgcctta aagggattac
    10981 cgtgacaaaa agtatttcgt taagtaaacg catctccgtt attgtgattc tttttgccat
    11041 tgtggcagtg tgtactttct tcgtgcaatc ttgcgcccgt aaaagcaatc atgcagcata
    11101 atttcagaat taccacgcca ccattgacgg caaagaaatt gcaggcataa cgaataatat
    11161 ctcttcgctg acctggtcgg cacaaagcaa tactcttttt agcactatca acaaacctgc
    11221 cgccattgtt gaaatgacca ccaatggtga ttttatccgc acaattcctc tggattttgt
    11281 taaggatctc gagacgatcg aatacattgg cgataatcag tttgtcatta gcgatgagcg
    11341 cgattacgcc atttatgtga tttcactgac cccaaactcg gaagtaaaaa tcctcaaaaa
    11401 aataaaaatc ccgctgcaag actccccaac caattgcggt tttgaagggt tggcttattc
    11461 tcgccaggat cacactttct ggttttttaa agagaaaaat ccgattgagg tctacaaagt
    11521 taacgggctg ttaagtagca atgaattaca catcagcaaa gacgaggcac tacaacggca
    11581 atttacttta gatgatgtgt ccggcgcaga atttaaccag caaaaaaata cgcttctggt
    11641 gctgtctcat gaatctcgcg cactccagga agtaaccctg gttggcgagg ttatcggtga
    11701 aatatcacta accaaaggta gtcgaggttt gtcgcataac atcaaacagg ccgaagggat
    11761 tgcgatggat gcctctggta atatttatat cgttagcgaa ccaaatcgtt tttatcgctt
    11821 tacccctaaa tcatcgcatt aacaggctgg catatttcac ctttttgtta ttaacttgtt
    11881 aacaacaaaa atgacataca aggttaaaca tgccttcgtc ctcgcatccc gtagaacgct
    11941 tttctttcag caccgcgctt ttcgggatgc tggttctgac cttaggtatg ggattaggtc
    12001 gcttccttta tacgcctatg ttgcccgtca tgatggcgga aggggcgttt tcatttagcc
    12061 agctctcgtg gattgccagc ggcaactatg cggggtatct ggctggcagt ctgctatttt
    12121 cgtttggcgc atttcaccag ccgtcgcgcc tgcgcccatt tctgttggtt tctgccttgg
    12181 cgagcgggtt gttgatcctc gcaatggcat ggttgccgcc atttattctg gtgttattga
    12241 ttcgcttcct ggcgggtgtc gccagcgccg gtatgctgat ttttggttcg acgctgatta
    12301 tgcagcacac gcgccatcct tttgtgctgg cagctttgtt ttctggcgtt ggcattggca
    12361 ttgcactggg caacgaatat gttctggcag gcctgcattt tgccttatcc tcgcaaacgt
    12421 tatggcaagg cgccggggcg ctttctggca tgatgctgat tgcacttacg cttttaatgc
    12481 cctcgaaaaa acacgccatc gcaccaatgc cattggcaaa aacggagcaa cagataatga
    12541 gctggtggtt actggctatt ctgtacggcc tggcgggatt tggttatatc atcgtcgcga
    12601 cttacctgcc gctcatggcg aaagatgcag gctcaccatt gttaaccgcc catctctgga
    12661 cgttagtcgg cttatcaatt gtgccaggct gctttggctg gctatgggca gcaaaacgtt
    12721 ggggggcttt gccttgcctg acggcgaatt tgctggtgca ggctatcagt gtgctgctta
    12781 ctctcgccag cgactctcct ctcttgctta tcattagtag tatcggtttt ggcggtacct
    12841 ttatgggcac gacatctctg gtgatgacta tcgcccgcca actgagcgtt ccgggaaatc
    12901 tcaatctttt aggctttgtg acgctcattt acggcattgg acaaatcctc ggcccggcgc
    12961 tgaccagtat gctcggcaat ggaacatcgg cgcttgccgg cgccactctc tgcggcgcgg
    13021 cggcgttgtt tattgctgcg cttatcagca cagtacaatt atttaagctg caagtggtca
    13081 cttcatgagg agtgtttccc ttaccactca agaaattcga ccgcaaccgt tgatgtcagc
    13141 cagaccccgt tttccgtttg ccagaaaggg atgcctcgtt tcgccatttc ctgtgctttg
    13201 acggttaaga taaccggcga tccgtgtctt gctcccactt tgcgtgccgt tgcttcatca
    13261 gcagagagat gtacgtagtg acgctcacct gcaatcagcc cctgtttttt tatctcgtca
    13321 aggaaacggc ttgccgtacc gtggtaaaga aactgtggcg gtgttttttc ggcaaaggaa
    13381 atagcgacct gcgaagtcaa atgcccctga accgcgcgga tgcacctacc atcactggaa
    13441 taactaaaac gctttttatc actggtggcg accacggtat ccagcaatgt acgggttaat
    13501 cgcttgcccg ctttttgggc acaaagtatg agcttatcga tatccgccca tccttcacgg
    13561 tcaagtacga taccaatcgc ctccggtttg tgtcggagga caaaacttaa aaacttgctc
    13621 gtatccgcca gctctttttc gttgtacttt gccatctcgt tttcgtccct ggattttctg
    13681 cttttattta ctgatgtcat tgtcactggg tttatgaatt ttctgcaact caatcctgcg
    13741 cagagccttt tccacacagc gtaatttttc cgctatgcaa aaaacgcata gccagaaaca
    13801 tacttgcatt ttccccatac ccacatggcc gataaggttt ttcagctcca ggaattttcc
    13861 tggctcaagc aacacacaaa cacaaaaaaa caatggggat tgttatgaca caacaagggg
    13921 atgctgtagc cggggatctg gcgacagaaa aagtgggcat taaagggtat ctggcttttt
    13981 ttctcaccat aatctttttt tccggtgttt tttccgggac agacagctgg tggcgcgttt
    14041 ttgatttttc cgttctgaac ggatcgtttg ggcaactgcc tggtgcgaac ggagccacca
    14101 cctcatttcg cggggcgggt ggcgcggggg ctaaagatgg ctttctcttt gcgctggagc
    14161 tggcaccgtc agtcattctc tctctgggga ttatttcaat taccgatggt cttggtggat
    14221 tacgcgccgc gcagcagtta atgacacctg tgctaaaacc gttgttgggc attccaggga
    14281 tctgctcgct ggcgctgatc gctaacctgc aaaacactga tgccgccgcc ggtatgacca
    14341 aagagctggc gcaggaaggg gaaattaccg aacgcgataa agtgattttc gccgcatatc
    14401 aaaccagcgg tagcgccatc atcaccaact acttctcttc cggcgtcgcg gtgttcgcct
    14461 ttctgggcac gtccgtaatc gtacctttag ccgtcatcct ggtgtttaaa tttgtcggag
    14521 ccaacatttt gcgcgtctgg cttaacttcg aagaacgtcg caacccaacg caaggagcgc
    14581 aagcatgaca actcaggtcc gcaaaaacgt catggatatg tttatcgatg gcgcgcgccg
    14641 tggctttact atcgccacga ccaacctgct gccaaacgtg gtgatggcgt ttgtcatcat
    14701 tcaggcgctg aaaattaccg gcctgctcga ctgggtgggg catatctgtg aacccgtaat
    14761 ggcgctgtgg ggattacccg gtgaagcggc aaccgtcctt ttagccgcat taatgagtat
    14821 gggtggcgca gtcggtgttg ccgccagtct ggcaaccgct ggcgcattaa ccggtcacga
    14881 tgtcaccgtt ttactgcccg cgatgtattt aatgggcaac ccggtgcaaa atgtcggtcg
    14941 ctgcctgggg acagccgaag tgaatgccaa atattacccg catatcatta cggtctgcgt
    15001 gattaacgca ttgctgtcga tctgggttat gcagcttatt gtttaacaag gagttaccat
    15061 gattgattat accgcagccg gttttaccct gctgcaggga gcgcatttgt atgcgccgga
    15121 agatcgggga atttgcgatg tcctcgtcgc taacggcaaa attatcgccg ttgccagcaa
    15181 tatcccttct gacattgtac cggactgcac ggttgtcgat ctcagcgggc agatcctctt
    15241 tcctggtttt attgatcaac acgtccattt gattggtggt ggcggtgaag caggtcccac
    15301 gacgcgcacg ccagaagtgg cgctaagtcg cctgacggaa gcgggcgtca cgtcagtggt
    15361 tggtctgctg ggcaccgact ctatctctcg ccacccggaa tccctgctcg ccaagacccg
    15421 tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc
    15481 ccgcaccatt acaggttccg tggaaaaaga cgtggcgatt atcgatcgtg tgattggcgt
    15541 gaaatgcgcc atctctgatc accgttctgc cgcaccggac gtttatcacc tggctaatat
    15601 ggcggcggaa tcccgcgttg gcggtttgct cggcggtaaa cctggtgtca ccgtgttcca
    15661 catgggcgac agcaaaaagg cgttacagcc tgtctatgac ctgctggaaa actgcgatgt
    15721 gccgatcagc aagttgctgc cgacccacgt taaccgcaac gttccgttgt ttgagcaggc
    15781 gctggagttc gcgcgcaaag gcggcactat cgatatcacc agcagcattg acgaaccggt
    15841 cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt attccgctgg cacgcgtcac
    15901 cctcagctcc gacggcaacg gtagccagcc gttcttcgat gacgaaggga atttaaccca
    15961 tatcggtgtt gccggttttg aaacgttgct ggaaaccgtg caggtgctgg tcaaagacta
    16021 tgatttcagt atcagcgatg ccctgcgccc gctcaccagt agcgtagccg gtttccttaa
    16081 cctgaccggg aaaggcgaaa ttctgccagg caatgatgct gacttgctgg tcatgacgcc
    16141 agaactgcgc attgagcagg tatacgctcg cggcaaactg atggtcaaag acggcaaagc
    16201 ctgcgtgaaa ggaacgtttg aaacggctta aacggttgtc agccgcccga atggacgtag
    16261 aatgcccgca agggcggctg acagagtaaa acgtaatgga tgactgtggt gcgattttgc
    16321 ataatattga aaccaaatgg ctttatgatt ttctgaccct ggaaaaatgc cgcaattttt
    16381 cccaggcggc agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc atccgtgcgc
    16441 tggaacaggc gattggtgtt gaattgttta accgccaggt aacgccgctg caactctcag
    16501 aacaagggaa aatcttccat tcgcagatcc gccatctgtt gcaacagtta gagagcaatc
    16561 tggcagagct gcgtggcggc agcgattacg cgcaacgtaa aatcaagata gccgctgcac
    16621 actctctttc cctcgggctg ttaccgtcca ttatcagcca gatgccgccg ctctttacct
    16681 gggcaattga agctattgat gtcgatgaag cggtcgataa actgcgtgaa gggcaaagtg
    16741 actgtatttt ttcctttcac gacgaagatt tgctggaagc gccgtttgac cacattcgct
    16801 tatttgaatc tcaattgttc cctgtctgcg ccagtgacga acacggagaa gcacttttta
    16861 acctcgcgca gccacacttt ccgttactga attacagccg caactcctac atggggcgat
    16921 tgattaatcg caccctgacg cgccacagtg agttaagttt cagcaccttt tttgtctctt
    16981 cgatgagcga gcttttaaag caggttgccc tcgacggctg tgggattgcc tggctgccgg
    17041 aatacgctat ccaacaagaa attcgcagcg ggcagctcgt tgtgctgaat cgggacgaac
    17101 tggtgatccc gatccaggat ctacgcatac cggatgaata ctcgcatgaa tcccgttgcc
    17161 gaacgcttct ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca ttctccagca
    17221 tcgcactaca ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt tcaccgtcgt
    17281 ggtacagcat tatctcaagg ctgatcgcca gagtgtggca gtacgcttct ttaaaattaa
    17341 cctgcaacgc cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc tgacggcaat
    17401 accagctctg gagatgtttg tcgctctggt tatgcacttg ccactcgggc atgcctgaaa
    17461 caagtaatga cggtaatgac gaatgaggag aattaaaaag agtgaggaga tcgctgcata
    17521 ccgatttacg cagcaatgac gttttattgc ctgaaatttc aggtaaaaca gcctgacgtg
    17581 attggcgcat cattttgcgc actcctgacg tttagcaaca tatgcggctt gccctgaaca
    17641 ccgggcacca ctgtattgat gctcattttc catagccatt cccttttgtt cattacatcc
    17701 atgtcagtct gagacaggcc tgaatcctgt cagcaaagta taaaacctta cttgcctatg
    17761 aatatctact gacttttcag gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa
    17821 aaaaac
//
LOCUS       DAZKGS010000087        17750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKGS010000087 DAZKGS010000000
VERSION     DAZKGS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(267..2900)
                     /gene="mngB"
                     /locus_tag="ACHOQY_002968"
     CDS             complement(267..2900)
                     /gene="mngB"
                     /locus_tag="ACHOQY_002968"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP9679026.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2918..4834)
                     /gene="mngA"
                     /locus_tag="ACHOQY_002969"
     CDS             complement(2918..4834)
                     /gene="mngA"
                     /locus_tag="ACHOQY_002969"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP9679027.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            5009..5731
                     /gene="mngR"
                     /locus_tag="ACHOQY_002970"
     CDS             5009..5731
                     /gene="mngR"
                     /locus_tag="ACHOQY_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP9679028.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5835..6704)
                     /gene="sucD"
                     /locus_tag="ACHOQY_002971"
     CDS             complement(5835..6704)
                     /gene="sucD"
                     /locus_tag="ACHOQY_002971"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9679029.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPLPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6704..7870)
                     /gene="sucC"
                     /locus_tag="ACHOQY_002972"
     CDS             complement(6704..7870)
                     /gene="sucC"
                     /locus_tag="ACHOQY_002972"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9679030.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7964..9181)
                     /gene="odhB"
                     /locus_tag="ACHOQY_002973"
     CDS             complement(7964..9181)
                     /gene="odhB"
                     /locus_tag="ACHOQY_002973"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9679031.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9196..11997)
                     /gene="sucA"
                     /locus_tag="ACHOQY_002974"
     CDS             complement(9196..11997)
                     /gene="sucA"
                     /locus_tag="ACHOQY_002974"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9679032.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12298..13014)
                     /gene="sdhB"
                     /locus_tag="ACHOQY_002975"
     CDS             complement(12298..13014)
                     /gene="sdhB"
                     /locus_tag="ACHOQY_002975"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9679033.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(13030..14796)
                     /gene="sdhA"
                     /locus_tag="ACHOQY_002976"
     CDS             complement(13030..14796)
                     /gene="sdhA"
                     /locus_tag="ACHOQY_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9679034.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14796..15143)
                     /gene="sdhD"
                     /locus_tag="ACHOQY_002977"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14796..15143)
                     /gene="sdhD"
                     /locus_tag="ACHOQY_002977"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9679035.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15137..15541)
                     /gene="sdhC"
                     /locus_tag="ACHOQY_002978"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15137..15541)
                     /gene="sdhC"
                     /locus_tag="ACHOQY_002978"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9679036.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15922..16029
                     /locus_tag="ACHOQY_002979"
     CDS             15922..16029
                     /locus_tag="ACHOQY_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679037.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16235..17518
                     /gene="gltA"
                     /locus_tag="ACHOQY_002980"
     CDS             16235..17518
                     /gene="gltA"
                     /locus_tag="ACHOQY_002980"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9679038.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4238 a         4807 c         4390 g         4315 t
ORIGIN      
        1 taattaactt ataattatat tataattaac tcacccagag ttattatttt gttaaaaata
       61 aagccacata tacgtggctt gagagtgaag ataaacgcaa aattacctga tgcgctacgc
      121 ttgccagtgt ctacatcatc tctgcaatat attgaatttg cctgcttttg tagaccgaat
      181 aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc
      241 atatgtggct tcggtttgat tgctattcag gcaagccggt aactgaacgt ccgtgactgg
      301 ccgggtaaaa aaggccccga tagatttgaa ccttgatttt cctcggtggt aatgtgttca
      361 tccatcatcg tttctgagca agaaatcacc tcgcgactga aagcaacagt cgcatcacag
      421 gttgctgatt cagccggatt aaacagccgt aaaatcactt cttgtcggtc ttcagctttc
      481 ttaagtgcgc ttatcaggca tcccactggg ggcattttca acaaactata actttccggc
      541 acgttgaatc cggctttgtt gagcttcatc gcatcccatg ggattttgtt gtaacactgt
      601 actggagtca gccatgctcg cgcctgctga gctacaccag cggcggttgg cgtaccggta
      661 taactcaata aacttaggcg acaagaaagc agaccacgta gttgtgagtc cgggactggc
      721 attttaattc ccgaaggccg cccaggcctt aaaagcagat cttctttgcc cagtaagccc
      781 acgccacgca gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac ttcaaattca
      841 cgtagccctt cgctaaagac agccatgccg ttacgccctt cctgtaaggc aacatagttg
      901 agcatattcc ataccggaac cggcgcttct ttccagcctt cttgctgcca gttgttcatt
      961 gcactgtcgt tcacggggcg cgttagcgaa ccaaactgcg tatctgccag aacactgtcg
     1021 gtgttaaaag gtgtagggat caggacgcga aggcgatgat cgtcagcctg gttatcaagg
     1081 ttgatatcca catcaatacg tctgctgtta tgactcagag tgacaaccat ctccacccct
     1141 actctgccag tggattgtct tgcgctgcgt tcttgcagat tacgcggcac cgccatttca
     1201 taacggataa cagccctgct ctgccaggct tcatgaataa tatcgcattg cggtttcgcg
     1261 ttcgccgcgg taattaccca ctcttctttt gcgggtgaat agtcatattc atcaccatca
     1321 tcagagcttt cttcaatttg caatacccgg tcatagcgca caccgctgtc tttatctacc
     1381 agttgcagag aaccatcctc attgagcgca atctgccaga aagcattttc cagaatccct
     1441 tcagcgtcac tttttgccgc aatcacgttg ccaggctgat tcgcttcaat ataaagcgtg
     1501 cgatagccca tagaagggac aatctggttg atctggatgt cgaactccat aaagggatca
     1561 taattgccat aatgaacaat ttgccgatcg attaggcctg gatcgatctc acgggcatgg
     1621 cgaataaaat acggcacagg ctggccgcga tcgtcccgca aattaaactg gctggcgcgc
     1681 aggcgcacag tggtgttgat cacttcttca cgcggccagg gcatcaggtt aaacaggacg
     1741 agtttgtcgg cgtcgctctg cggcatgttg tcggcaattt tgcgcatgta gaaacgaatc
     1801 agattatccg ccatgtcttc agccagttcg aagcgggcga cgatttcgcg atgaactttg
     1861 tcactgcaac aacaaccgat actgtcgtgg gcatgatttt ttaagatctc tttccacatt
     1921 ttctccagca agccgtggtg gtattcaaaa cccaacgtcc aggccagtgt tgccagcggt
     1981 tccagcagat taacaatctt attttcaata cgcgcgtggg caattttgat atccatacgc
     2041 gtagaaccga tggtgcgatg cacgcgcata tatttgccat caataaattc ccctttcagg
     2101 gttgccagat tatctcgctg cgcttcgatc ttctcaaata cctcttcaaa gcggctcatc
     2161 acaaacttac gttgagggta gatctcacgt agcttatcca tcacttcgaa gatattttgc
     2221 tgcaatggca tctggtcatg accattcggc aataaaattt ctttggttac tgacgctttt
     2281 tccagcacat caaaataact gtcgaggcgc ttgcgtaatc cgtcttcgtc ggcaggtaag
     2341 tacttaccga tggcgtagcc cagcggcaac acttgcgccg tcacttcgct accgtcactg
     2401 ctttgccaca aaaactcggt tttatcagta ccgtggcgct cagaacatcc acgccagaac
     2461 atggtgcggg taatgccaaa tccattgtag atatgcggaa gctgcccgga catgccaaag
     2521 gaatccggta aataacctat tttcatcggc tcgccaaacg cgaggcagtc acgcattccg
     2581 tacatcagat tacggacgat ggattccgca gaaacaatcg tggtatcggt ctgggtatac
     2641 caggggccga taatcaactt gccggcttct acctgtttct tcacacggtc tttgttttcc
     2701 ggtttcaccg cgaaataatc ttcgaggatc gccgtttgcc cgtcgagtac gtaatatttg
     2761 tattcgttgt cctgttccag tcggcacagg atctcttcca tattattgac cagcagaatg
     2821 cgtgactctt cggtggtgaa ataccactcc cgatcccagt gcatatgcgg ggtgatgtga
     2881 acgcgagata ccgctttcat cgtcgtttcc tttttgttta tggcattacg ccatcagtca
     2941 gatagttgcc atgcttaacc gcgtgacgcc gccacatcag gagaattgca gtcgagattg
     3001 cagcccccac caatgccgca ccaaaccagc caattgccgc cataacaccg cccgcgccat
     3061 tatcatgaag taaaaagagc gagaaaatgc cggcaccggg tgtcgaaagg ccgatattca
     3121 tcgcaccgac aatagcgccc gttaccatag agcccagcac aaacgaacca ataacccgca
     3181 gcggatcttc aatcgccatc gggatcgccc cttcggtaat acctgccagc cctaacagcc
     3241 aggtggattt cccggtctca atttcaaact ctttaaacag gcgcggtgcg agcatcgtgg
     3301 aagcggttac ggtaaatgcc gaaaccattt tgacggaggc gaaaatggca tacgggccgt
     3361 aaacgccgtt cgccattgcg cccaggcaga atgcataagc ggctttattc actggccctc
     3421 caaggtcaaa ggaacacata aaaccgagaa tggcacccag caacagcgcg ttacttcctg
     3481 acagaccgtt cagccaggcg gtaagcgagt tattgatcca tgcgacaggt tctcccacca
     3541 caaacagcat cagactgccc gctcccaacg taccgagcac cgggtagaga taaaaagtca
     3601 ggaagccatt gaatttactg ccaagacgca agtgattttt cacccagcgc atcaagtaac
     3661 cggctatcaa tccgccaacg accgcgccga gaaacccgga gccgatcatg ttggcggcaa
     3721 gtccagccgc aaagcctggc gctaacgccg gtttatctgc cagagaatag gcggtatagg
     3781 ccgcgagcac cggtaccatc aaaattccga gcagcccgcc gcccagcttg cggtacatcc
     3841 acagccagga gttttcttca ttaaacagat cttgtagccc gaagatttgc gacagtaata
     3901 ccgcgaccgc cagcaccgtg ccccccgcga caatcaacgg tacggcaaaa gagattccgc
     3961 tcaacagtgc ctgtttcagc tccgttttga cacttttcac cggttgcgta tcttgctgta
     4021 cggtacgcgt ctcatcgcta cgcttgaggg taagcgcttg ttggatcaac gcttctgcat
     4081 ggcgaatcgg ctcggcaaca ggcactgaaa gcgcgggaat gccattaaaa cgctcactct
     4141 ccttgatggc gacttcagcc gcaaaaatac aggcggttgc actattgagt tgatccgccg
     4201 ttaaacgccc ttcaatgccg ttagcgcctt gtttttcaac gtaaacattt acgccgagtt
     4261 tgcgtccggc tttttccaga tattccgcag ccatataggt gtgagcaata cccgccggac
     4321 aggccgttac gcagacgata gttggcgcgt tggaaaaaga ggcagaaggt tgcgtgcctc
     4381 ccttgtcatc cagcgccgag agcaactcat caggcgtcgt cgccgactgt atacgcgccc
     4441 gaatctcatc atccgccagg cgcgtggtca gcgctgtcag cagttgcata tgcgtagtac
     4501 ccgcttcatt ggggggaatc gccagcagca ccactaaatc aaccgcttcc gggccatcaa
     4561 cgccttccca ctgaagcggc tcgctgagtg tggcgaccgc aaacgccgct tcttttaccg
     4621 cagcagtttt gccatgcggc acagccaacc cttcacctaa ggcggtcggg ccaaggcttt
     4681 cacggcgata cacttcttcc agaaattgct cagtactgga aattttcccc agagcagcaa
     4741 gacgttgagt caacgcgtgg atggcctctt cacggctggt aaagcgcgca ttcagacaca
     4801 acgcatcgcg gtgggtcaga gtcgtcagat tcatcatgat cctcacttga tcgtttttat
     4861 agtcgcgcca gtgtgcccga taaaacaata ccattatgtg atcaatttca cttgattgaa
     4921 attatttgta ttgtatttgt atttgtatta tttacaggat gaatttatcg ggcataatgg
     4981 ctaaattcgt attaatgaga tactaaaaat gggacacaag cccttatacc ggcagattgc
     5041 cgatcgcatt cgtgaacaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacctac
     5101 cgaatcggca ttacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg
     5161 ccagttagtc gagcaacaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga
     5221 agagcgggtc aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg
     5281 tcacgtcgat acacacagtg aagtcctgat atttgaagtg attccggctg acgattttct
     5341 tcagcaacag ctacaaatca ccgcgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta
     5401 tcgcaagcaa aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga
     5461 tctcacctgg caggtcatgg aaaattcaaa atatcacttt attgaagaag tgaagaaaat
     5521 ggttatcgat cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg
     5581 tctgcttaat atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt
     5641 tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac
     5701 cctgatagcg caacgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca
     5761 accatgtcga aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt
     5821 cttattacag atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg
     5881 cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc
     5941 ccaccggcaa tgatggcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca
     6001 gcgatgtaac ccacaactgg cttggtaaca tgctctttga tgtacgctgc cgcttcttct
     6061 tcagcgctac cgccgatctc accgatcatt acgatcgctt cggtctgcgg atctttttcg
     6121 aacatttcga gaatgtcgat aaagttagag ccagggagcg ggtcaccgcc gataccaacg
     6181 caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta
     6241 ccggaacggg aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg
     6301 attttgcatt cacccggagt gataacgccc gggcagttcg ggccgatcat acgaacgccg
     6361 gcttcgtcca gtttcacttt cacagtcagc atatccagcg tcgggatgcc ttcagtgatg
     6421 gtgataatca gtttgatgcc tgcatcaata gcttccagaa tggagtcttt gcagaacggt
     6481 gctggtacgt agataacaga agcggtagcg ccagtagcag caacagcttc acgcacggtg
     6541 ttgaacaccg gcaggccgag gtgggtagta ccgcctttac ctggggttac gccgccaacc
     6601 attttagtgc cgtatgcaat ggcctgttct gagtggaaag tcccctggct accagtaaag
     6661 ccctggcaga taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg
     6721 cggcaacaac ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc
     6781 tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg
     6841 gtacgttaac acccacttct gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac
     6901 gaacgatacc gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga
     6961 ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc gccaacgtca aggaagttag
     7021 ccggttcgcc gccgtgcagt ttaacgatgt ccatcgtacc catcgccaga cctgcgccgt
     7081 taaccataca accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct
     7141 gtgcttcacg cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga
     7201 acagtgcgtt gccgtcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg
     7261 tgatgaccag cgggttgatt tcgatcaacg ccaggtcgcg ctccaggaaa atggtcgcca
     7321 ggcccatgaa gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg
     7381 ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttgtgga
     7441 tcaggtgcgg agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca
     7501 taaagaccac acgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga
     7561 tatcggtcgc tgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat
     7621 acgttaccag acgcttgccg agccagtttt ctgcaaaagc acggatgtct tctttgctgt
     7681 ttacaacttt cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta
     7741 cccacgggcc ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg
     7801 cataacccac cggtgctggt aagccatagc gggcaaaaag ttgttttgcc tgatattcat
     7861 gtaagttcat gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac
     7921 accctatcca tgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca
     7981 gacgcgtcgg atcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac
     8041 catcgatcag acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct
     8101 gaccattcac cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg
     8161 gcgggttgat gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga
     8221 agttaccacc ggtcagatct tcaacggtca gcttgccgtc acggcctttg actgccagct
     8281 ctttgatttt cttctcgatg tctgccatgc cgagggtatc gacatcacgc agtaccggtg
     8341 tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca
     8401 cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg tttcagggct tcaaccaccg
     8461 ctttcacgta gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac
     8521 cgtactgctt acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca
     8581 tggcggtgga gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca
     8641 tcgggacacg tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg
     8701 ccggagcaga ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca
     8761 gacgaccacc cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca
     8821 ggcgacggat cgccgggctt aacgcatcgt tgttttgctc ttccagagat gcctgctggc
     8881 gttgcgccgg agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt
     8941 tgccttcacg caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca
     9001 gaaccgcatc cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt
     9061 cgatttctac cagcacttca tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg
     9121 cgacggtggc atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca
     9181 ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt
     9241 ctggtgaacg gacatatacc ctaccgccgg agaggcggag gccgggcggc ctgcataacg
     9301 cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc
     9361 ctggttgagc ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa
     9421 cacttcctgc atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac
     9481 atcgtgttga ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca
     9541 catcactacg cgcttcacgc ccttcggatc aagctcgtcg atttcaccga tggctggcag
     9601 gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga
     9661 tttcggcgac atcacgacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat
     9721 gtggtaaacc tgtgccgggg tagacggtac gcacacctgc atgttttgct cagcacaaag
     9781 ttgcagataa cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg
     9841 cggcagcaac atcaccagac cacacatccg gccccatttc tgttcgccag aagagatgaa
     9901 ctggtcgata accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt
     9961 cagagtgcgt ggttctgcgg tggcataacc gtattcaaac gccagcactg cttcttcaga
    10021 cagtacggag tcccagacgc ggaacgcgcc ctgcccgtta tggatgtgtt gcaacggcgt
    10081 gtaagtggaa ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg
    10141 accggagtct tcacccgaca ggcgaaccgg aatgccttcg tcaaccagcg tggcgtaagc
    10201 gaggttttcc gcaccgcccc agtcgaacag tttctcaccg gcagccatcg cctggcgatc
    10261 gccataaatc ttggcaacgc gagactgcat ttcaactgct tccggcaccg tgctgatgcg
    10321 tttcgccagc tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc
    10381 gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac
    10441 tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc
    10501 cagcgtcgcc actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg
    10561 ttttttgatt ttctgataca tcagcggctg ggttgcgctc ggctcgtcgg cttcgttgtg
    10621 gccgtgacgg cggtagcaca ccaggtcgat gaagacatca cgtttaaagg tgttacggaa
    10681 atcgagcgcc agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa
    10741 aatcggtgcc tgaaccatct taccgatatc agtacagtac ggtgtagaac gagcatccag
    10801 cggattagag gtggtgaaac caacctggtt gttgataacg atacgtaccg taccgccaac
    10861 ttcataacca cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac
    10921 tgcggcgtca ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct
    10981 gtccagacgg gcacgaacag aaccgataac taccgggctt acaatctcaa ggtgagacgg
    11041 gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc
    11101 catgtggtat ttcacgtcac ccgtgccgag gtgttcttta tgtttaccgg caaactcgtc
    11161 gaacaagtct tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg
    11221 cgccatccca agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt
    11281 aagcatcggg attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt
    11341 tgcgccgagg taacgttcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt
    11401 ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc
    11461 ttcggtgctg gtaatgtgca tatactcggc accaatcggg ccacagtagg tttgcttgag
    11521 ggcttccagc agctcgccga gtttcatggt ttctttgccg ctggcaaatg aaccgacgtt
    11581 gaaggtctcc tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac
    11641 tttatcttgc tgccacagtc ccagcggatc gagattcgca tgctggtgac cacggaagcg
    11701 gtatgcgtta atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat
    11761 cgttgaagag taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga
    11821 gtggaattga tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt
    11881 agcgtcaacc gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg
    11941 gtttgcgcca gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt
    12001 gatcccttaa gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg
    12061 tgcgtgccgg ggttcacttg cgagctcttg tctatgtagt ggctcgcgaa ggaaccttta
    12121 aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct
    12181 gatgcgctgc gcttatcagg cctacgggta atgacttgta ggccggataa ggcgttcacg
    12241 ccgcatccgg cactggttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacggttta
    12301 cgcattacgt tgcaacaaca tcgacttaat atggccgatg gcgcgcgtcg ggttcagccc
    12361 cttcggacat acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc
    12421 gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata
    12481 tgccgctaac aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca
    12541 agaggttgaa caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg
    12601 ctctggcatc tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg
    12661 cttaattttg tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac
    12721 cggtaaacct ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat
    12781 cggggtaata caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac
    12841 accttcacgg caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag
    12901 cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg
    12961 cggagcatca tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct
    13021 gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat
    13081 gttgacgctt cggcgcgtca tggattccga ctctggcaga tacagggagt ggcacagcca
    13141 gttttcatca tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg
    13201 gaagttggca gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca
    13261 ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg
    13321 ctcgcggatc actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac
    13381 cgagaagtta tgctgcatac attcttgcag cgctttacga atcgccaccg gatcttcacc
    13441 gttacgatta ttgttccagc ggttcaggcg atccagagac acttcaacat cagactcgct
    13501 ggcatcgcgc agtgcgccct gctcggcgat agactcttgc agatgcagac ctgccgcgcg
    13561 accaaagacc accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga
    13621 tacacaagcg atttcaccaa cggcaaacag tcccggaaca accacatctt cgcctttctc
    13681 attcacagtc agcgcctgac cggtaacttt ggtcggaata ccgcccatca tgtagtgaca
    13741 ggttgggata accggaatcg gctctttcac cggatcgacg tgagcgaagg tacgggaaag
    13801 ctccaggata cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag
    13861 tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat
    13921 ggaacgcgcc accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat
    13981 aaagcgttcg ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac
    14041 cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac
    14101 cggtacgcct gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt
    14161 ggtggactga taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa
    14221 ataaaccact tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg
    14281 gtttttcacc agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt
    14341 ctgctgataa agcgtgtgca acagtgcgtg accggtacgg tcagccgccg ccgcagtgcg
    14401 tgccgcctgc tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg
    14461 accatcatca agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg
    14521 cccggtttta cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac
    14581 ggtgtcgtac atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat
    14641 gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca
    14701 ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat
    14761 cacaactgca tcaaattctc tgactggcaa tttcatcaca caccccacac cacaacgaat
    14821 ccataaatca cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaat
    14881 gccagcggtt taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag
    14941 atagaaaaca gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc
    15001 cagacttcat atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag
    15061 agcgtcagga cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct
    15121 aatgcggagg cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa
    15181 taacaaagga gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa
    15241 aatccatcat catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc
    15301 cccacatgat aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt
    15361 caggggaaga gaggctggta cccagaagcc acagcaggat acccactgca ataaaggtga
    15421 tcacaccgga aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct
    15481 gtaggtccag attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca
    15541 tgctgttctt attattccct ggggactacg ggcacagagg ttaactttct gttacctgga
    15601 gacgtcggga tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttctataact
    15661 gcgcgtcatg caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc
    15721 tcgggattgc agggtgttcc ggagacctgg cggcagtata ggctgttcac aaaatcatta
    15781 caattaacct acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa
    15841 caacatttat ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa
    15901 cattaccagg aaaagcatat aatgcgtaaa agttatgaag tcggtatttc acctaagatt
    15961 aacttatgta acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga
    16021 caagtttaat aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat
    16081 cctttacctg caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc
    16141 gagccaaata acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc
    16201 agtcttacgt aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac
    16261 cctcaacggg gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt
    16321 tattgatatc cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc
    16381 aaccgcatcc tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca
    16441 ccgcggtttc ccgatcgatc agctggcgac cgattctaac tacctggaag tttgttacat
    16501 cctgctgaat ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac
    16561 ccgtcatacc atgatccacg agcagattac ccgtctgttc cacgctttcc gtcgcgactc
    16621 acatccaatg gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc
    16681 gctggatgtt aacaatcctc gtcatcgtga aattgccgcg ttccgcctgc tgtcgaaaat
    16741 gccgaccatg gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg
    16801 caacgatctc tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc
    16861 gtatgaagtt aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga
    16921 ccatgaacag aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc
    16981 gtttgcctgt atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa
    17041 cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt
    17101 tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta
    17161 caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga
    17221 gctgggcacc aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa
    17281 cgacccgtac tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat
    17341 cctgaaagcg atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac
    17401 cgttggctgg atcgcccact ggagcgaaat gcacagtgac ggtatgaaga ttgcccgtcc
    17461 gcgtcagctg tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg
    17521 gttgattgct aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata
    17581 tgcctaaaca caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa
    17641 aaaaaatata tcggtataaa ttataaacaa atcgatgatt tttaatcatc tatcttcctc
    17701 tcagttacat gaataaacca ccaaatatag tgcaccccta taatttccaa
//
LOCUS       DAZKGS010000088        17747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKGS010000088 DAZKGS010000000
VERSION     DAZKGS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..533)
                     /gene="ybcV"
                     /locus_tag="ACHOQY_002981"
     CDS             complement(123..533)
                     /gene="ybcV"
                     /locus_tag="ACHOQY_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFP9679039.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            834..1040
                     /locus_tag="ACHOQY_002982"
     CDS             834..1040
                     /locus_tag="ACHOQY_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679040.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1613..2140
                     /locus_tag="ACHOQY_002983"
     CDS             1613..2140
                     /locus_tag="ACHOQY_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9679041.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            2149..4248
                     /locus_tag="ACHOQY_002984"
     CDS             2149..4248
                     /locus_tag="ACHOQY_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9679042.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            4245..4457
                     /locus_tag="ACHOQY_002985"
     CDS             4245..4457
                     /locus_tag="ACHOQY_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679043.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4457..5965
                     /locus_tag="ACHOQY_002986"
     CDS             4457..5965
                     /locus_tag="ACHOQY_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9679044.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5910..7937
                     /locus_tag="ACHOQY_002987"
     CDS             5910..7937
                     /locus_tag="ACHOQY_002987"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9679045.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            8024..8347
                     /locus_tag="ACHOQY_002988"
     CDS             8024..8347
                     /locus_tag="ACHOQY_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP9679046.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            8340..8615
                     /locus_tag="ACHOQY_002989"
     CDS             8340..8615
                     /locus_tag="ACHOQY_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9679047.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8627..9205
                     /locus_tag="ACHOQY_002990"
     CDS             8627..9205
                     /locus_tag="ACHOQY_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9679048.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9202..9603
                     /locus_tag="ACHOQY_002991"
     CDS             9202..9603
                     /locus_tag="ACHOQY_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9679049.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9614..10357
                     /locus_tag="ACHOQY_002992"
     CDS             9614..10357
                     /locus_tag="ACHOQY_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9679050.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10418..10804
                     /locus_tag="ACHOQY_002993"
     CDS             10418..10804
                     /locus_tag="ACHOQY_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9679051.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10813..11142
                     /locus_tag="ACHOQY_002994"
     CDS             10813..11142
                     /locus_tag="ACHOQY_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9679052.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11114..14179
                     /locus_tag="ACHOQY_002995"
     CDS             11114..14179
                     /locus_tag="ACHOQY_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9679053.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14179..14508
                     /locus_tag="ACHOQY_002996"
     CDS             14179..14508
                     /locus_tag="ACHOQY_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9679054.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14518..15216
                     /locus_tag="ACHOQY_002997"
     CDS             14518..15216
                     /locus_tag="ACHOQY_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9679055.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15221..15964
                     /locus_tag="ACHOQY_002998"
     CDS             15221..15964
                     /locus_tag="ACHOQY_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9679056.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15862..16509
                     /locus_tag="ACHOQY_002999"
     CDS             15862..16509
                     /locus_tag="ACHOQY_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9679057.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16570..>17747
                     /locus_tag="ACHOQY_003000"
     CDS             16570..>17747
                     /locus_tag="ACHOQY_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9679058.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     "
BASE COUNT         4281 a         4089 c         5308 g         4069 t
ORIGIN      
        1 cctcgttttt tcggggtttt gttatatttg aatggttccg aatatctaaa tcacaattgt
       61 tgatggtttt tattaaacca atgcagtccg gctcaggagt gagagaagcc ggacgttatg
      121 gtttagcgtg gtaagatctg tgtagttttc tggatgcttt cagtaaatag taatgagtta
      181 tcaaaggcat agtaatatct ttggtgttcc tggatatttg taacccatcg gaaaactcct
      241 gctttagcaa gattttccct gtattgttga aatgtgattt cttttgattt caacttatta
      301 taggaggtct ctataagatg tttgtttctg gagaatttaa catttacaac ctttttgagt
      361 ccttttacta acactatgtt gtcgttttct aacacaatgt gaatattatc tgtggctaaa
      421 tagtaaatat aaagtgagac attgtgacgt tttagctcag aataaaataa ttcacagttt
      481 aaatctttac gcacttgatc gaatatttct ttaaaaatgg cagcctgagc cattggtaaa
      541 ccttccatgt gatacgatgg cgcgtagtta gcattatcgt gtttattgtt tcaatctggt
      601 ctgacctctt tgtgttttgt tgatgattta tgtcaaatat taagcctgtt tttaatgaat
      661 agtgttgatt gcgtaacaaa attgagcctt gctggcatcc aggagggata tgcaaccgac
      721 agatgtatgt aaggtcgatg tactcaaact ttcatacttt tcctctttta tgcagtaaga
      781 tttgaagtaa tattttaacc gctagatgac gagcaaacgc atggagcgac aaaatgaata
      841 aagaacaatc tgctgatgaa gtctcgttgg atctgattcg tgtaaaaaat atgcttaata
      901 gcaccatttc tatgagttac ccggatgttg taattgcatg tatagaacat caggtgtctc
      961 tggaagcatt cagggcaatt gaggcagcgt tggtgaagca cgataagaat tcgaaggatt
     1021 attccctggt ggttgactga gcaccataac tgctaatcat tcaaactatt tcacctgtga
     1081 cagagtcaat atcgcattct gtcactgtca ggctaataca gagctgcaat tcaactactg
     1141 caatgtcctc gtaattaggt gaatttacaa tatcgtcctg ttcggatgcc ggttgcattg
     1201 ctgaagatga ggcatttatg gttcgcatat tttcccctca tgctcgtcag tcctgtgcgt
     1261 aggaagaaac aggacactca cactaatttg tgtgggcatg ctgtgatgtc cttctgaatt
     1321 attcctatgc cattatgtaa agcgctgtat cagatgctca tcacggctgt caggctgtca
     1381 ggctgtcggg tcctcccggt gggggcccct gccacggggc gggagcgtcg cggaaaaagg
     1441 ctagtttttg catttccatg gcggcggcag catgtttggt aatttattga taattaaaag
     1501 ttatttctct tttcacctgt acaatatttc tttctccctg tcattagacc agtttgcaat
     1561 taattgaaat atataaataa acatgatttt cacctgccag atggagttgc ttatgtcaaa
     1621 tgtgagcggg atcggtgatg cttattactg gagtgttttt aaaatcgctg aggcctttgg
     1681 gcttcaccgg gacacagtaa aaaaacggct cctcgcggct aacactcctg tggctgcgac
     1741 tgtcaggggg aaccccgttt acgccctgca gcatgtcggg ccagccctgt ttagtgtgaa
     1801 gcatgaggca gcagactctg ttcatgatcc atcccgtatg gagccgaaag agagaaagga
     1861 ctggtaccaa tctgaaaatg aaaggatcaa gctggaaaag gagcagcgaa aactcatccc
     1921 cgttgatgaa gtagtcatcg tctattcgtc catgagaaag gctgtcgtcc aggttctgga
     1981 gacaattccg gatgttcttg aacgcgattg cgccctgact cctcaggccg tcggcgttgt
     2041 acagcaggcc attgatgacc tgcgatacac tcttcaggaa aaatcctacg aggcttgtgc
     2101 tgctgaatta attcctgatg aggaaggaga gagtctctag gaggaataat gggtttttca
     2161 tcagcccgaa atttgggaag ggacatatcg gcaggatttt ccccaccacg tcgcatgccg
     2221 atttcggagg ctgttaaaaa attcatgcgt gttcccaagg gggctggtaa ctcggtgcca
     2281 tgggatcctg aactgacacc ctacatcatt gagcccatga actgcctggc atcgcgtgaa
     2341 tacgatgcgg tgatttttgt tggtcctgcg cgaacaggga agaccattgg tctgatcgat
     2401 ggatggattg tctataccat cgtttgcgat ccttcggaca tgctcgttgt gcagatgacc
     2461 gaagataagg cccgcgagca ttctaaaaag cgcctcgaca gaacgttcag aagcagtgcg
     2521 gcggtaaaga aaagaatgag tccacgtcgt aacgacaata atgtccatga taagacgttc
     2581 agggatggct cgttccttaa aattggttgg ccctcggtca acattatgtc gtcgtcggat
     2641 taccggtttg tcgccttaac cgattacgac cgttttccgg agaatatcga cagcgagggt
     2701 gatggtttct ccctggcctc aaaacgtacc accacattta tgtccgctgg gatgactctg
     2761 gtggagagct cgccgggacg tgacatctgc gacagcaaat ggcgacgtaa gtcgcctcat
     2821 gaagcgccac cgacgactgg tattctttcc ctttacaatc gtggtgaccg ccgccgctgg
     2881 tactggtcat gtccgcactg cggtgaatat tttcagccag ccatggatgc catgaccggc
     2941 taccgtaatg aaccggatcc ctttaaagcc agtgaggcgg cgtatctact ttgcccgcac
     3001 tgcagcggca ttatcactgc ggagaaaaag cgtgagctca atagtgcagg agtctggttg
     3061 cgtgaaggtc aggtcattga tcgtaacggc aacgtttccg gtgaaccgcg ccgctcccgt
     3121 atcgccagtt tctggatgga agggccagct gctgcgtatc agacctgggc gcaactggtt
     3181 tacaaattac tgactgcaga acaggagtat gaagcgacag gaagcgaaga aacactcagg
     3241 gcggttatca acaccgactg gggattgcct tatcttcctc gtgccagcat ggagcaacga
     3301 aaaagtgaac tgcttgagca gcgggcagag ccagttcctt cccgcagtgt gccggatggc
     3361 gttaatttcc ttgtggcgac agtggatgtg caggcgggac gtcatcgccg ttttgtggtt
     3421 caggtaacgg gctatggcag tcgtggcgaa cgctggatta ttgatcgtta caacatcacg
     3481 cagtcattgc gcagtgactg cgacggggag agccagcgaa ttgatccggc cagctatccg
     3541 gaagactggg atgtcctgct gacggatgtt tttcataaaa gctggccgct ggcctccgat
     3601 ccttctcaac aaatgcgact gatggcaatg gcggtggact ccggcggtga agacggggtc
     3661 actgataatg cctataaatt ctggcgtcgt tgccgtcgtg atggccttgg taaacgtatt
     3721 tacctgttta agggtgacag catccggcgc gcaaaactga tcagccgtac attccctgat
     3781 aacaccggac gaacgggccg ccgggcgcag gccgcaggtg atgtgccgct ctggcttctt
     3841 cagacggatg ccctgaaaga ccgggtgaat aacgcgttat ggcgtgactc gccaggtcca
     3901 ggctatgtgc atttccctga ctggctgggg agctggtttt acgacgaact gacgtatgaa
     3961 gagcggagca gtgacgggaa atggagtaag ccgggtcgcg gtgccaacga agcttttgac
     4021 ctgatggtgt atgccgaggc tctggtcatt ctgcatggat acgaaaagat ccgctggccg
     4081 gatgcaccgg agtgggcgag ccgggagacc tggctggagt gtgtccagga cagtaccgaa
     4141 ccgtcatcct caccggaacc ggtatccacg cctgttaaaa aacaaaaacg gaagaaaaca
     4201 gtaactgacg atgttaaccc ctggctgact tccggaggat ggttatgaac cagaatgata
     4261 tcgaagccat gattcagcgt tatacggaag ctgaaatggc ggtgctggac ggaaaatccg
     4321 tcacctttaa tggtcagcag atgaccatgg aaaacttatc tgagatccgg cagggacggc
     4381 aggagtggga gcgccgcctt gcggctctga ttacacgacg acgggggcat cccgggtacc
     4441 ggctggcgag gttctgatgg caattcttga tgatgtgatt ggcgtttttt caccaggatg
     4501 gaaagcggca aggctgcgtt cccgtgcggt gatccaggct tatgaggccg taaaaacgac
     4561 gcggacacac aaagcccgac gggagaaccg aactgccgac cagttaagcc agtacggggc
     4621 cgtgtcgtta cgtgagcagg cccgttacct tgataacaac cacgatctgg ttattggtgt
     4681 atttgacaag ctggaagaac gggtggtggg gaaaaacggg attattgtcg agccacatcc
     4741 ggtattacgc aatggggcca ttgcccgtga cctggctgcg gagattcgca cccgatggag
     4801 tgaatggtct gtcagcccgg aagtcaccgg gcagtttacc cgtccgatgc tggaacgtct
     4861 gatgcttcgt acctggctgc gcgatggtga ggtgtttgcc cagatggttt ccgggcgcat
     4921 aaacagcctg gcgccttctg ccggtgttca tttctggctg gaggcgctcg agccggactt
     4981 tattcccatg actagtgatg agagcaacag gctgaatcag ggcgtgtttg ttgatgactg
     5041 ggggcgtccc gaaaaatatc tggtgtataa aagccgtccc gtatccggac ggcagatgga
     5101 aaccaaagaa gtggatgcag agcgaatgct gcatcttaaa tttgttcgcc gtctgcacca
     5161 gatgcgcggg acgtctttgt tgtccggtgt gctgatccgc ctcagtgccc tgaaagaata
     5221 tgaagattct gagctgactg cagcaaggat cgccgctgct ctggggatgt acatccggaa
     5281 aggcgacggg cagagctatg aaccggatgg taatggcaga aaggagaatg aacgtgagct
     5341 taccattcag ccgggcatta tttacgacga tctgaaaccc ggcgaagaaa tcggaatggt
     5401 gaagtcggat cgccccaatc ctaaccttga aacttttcgt aatggtcagt tgcgtgccgt
     5461 ggcggcgggc agtcgtctga gtttttccag tacagcgcgc aactataacg gcacttacag
     5521 cgcccagcgt caggagctgg ttgaatccac tgatggctac ctgatcctgc aggactggtt
     5581 tattggtgcc gtcacccgcc cgatgtatcg tgcatggctg aaacaggctg tggcatccgg
     5641 tgttatcagg ctaccccgcg atcttgaccg ttcttcactg tataccgcgg tgtattccgg
     5701 accagtgatg ccgtggattg accctgttaa ggaggctgag gcctggaaaa tccagattcg
     5761 tggtggagcg gcgacagaat cagactgggt acgtgctggt ggtcgtaatc cggatgatgt
     5821 caaacgtcgg cgcaaggccg aaattgatga aaaccgcaag ctggatctgg tatttgatac
     5881 cgatccggcc agtgataaag gaggcagtag tgccgcaacg aaacgacagg agccgcagca
     5941 caccgacgac cagtccgaag aataattcct ggttcaggat gcaggctggt caccagagtg
     6001 acgcggatat ttatatttat gacgagattg gtttctgggg tgttacagcg aagcagttta
     6061 tcagtgatct gaatgcactg ggcgatatca cccacattaa tctccatatt aattcaccgg
     6121 gtggcgatgt ctttgaaggc atcgccattt ttaatgcgct gaaaacacat ggtgcgtcca
     6181 ttaccgttta tgtcgacggt gtggcggcgt caatggcgtc ggtcattgcg atggtaggaa
     6241 acccggtcat tatgccggaa aactccttca tgatgattca taaaccattt ggctttacgg
     6301 gcggtgatgc ggaggacatg cgcacctatg ccgacctgct cgataaagtt gaggcggttc
     6361 tgttacccgc ttatgcacag aaaaccggga aaaccaccga tgaaattgct gccatgctgg
     6421 cggatgagac ctggatgtcc ggtgccgaat gtctggcaca gggatttgct gatcaggtga
     6481 caccagccgt taaggcaatg gcatgtattc agtcaaaacg tacagaggaa tttaaaaaga
     6541 tgccggaatc cattcgaaat atgattactc cgccacgcaa cagtgctcca cgcgtacagg
     6601 atgatggacc tgcagcctcc cggacgccag tgcaggcagc agcacccgtg gtggatgaaa
     6661 acagcatccg tgcgcaggtc ctggcagagc aaaaagcgcg tgtaaacggt attaatgatc
     6721 tgtttgccat gtttggcggg cgttatcagt cgttgcaggc tcagtgtctt gccgatcctg
     6781 aatgttcgct ggagcaggcc cgcgaaaagc tgttgaacga gatggggcgc gagtccacgc
     6841 catccaataa aaataccccg gctcatattt atgccggtaa cggtaatttt gtgggggacg
     6901 ggatccgcca ggcgctgatg gcgcgcgccg ggtttgaaaa aactgaacgt gataatgtct
     6961 acaacgggat gaccctgcgt gaatatgccc gtatgtcact gactgaacgt ggtattggtg
     7021 tttccagtta taacccgatg cagatggtcg gtgcggcgtt cacacacagt acgtctgact
     7081 tcggtaatat tctgctggat gttgcgaaca aagccattct gcagggctgg gaagatgccc
     7141 ctgaaaccta tgaacagtgg acgcggaaag gtcagttgtc tgattttaaa attgcccatc
     7201 gtgtgggtat ggggggcttc agtgctctgc gtcaggtgcg tgaaggggca gaatataaat
     7261 acgtcaccac cggagataaa caggccacta ttgcactggc gacctatggc gagctgttca
     7321 gtatcacccg tcaggccatt atcaatgatg atctgaatat gctgaccgat gtcccgatga
     7381 agctgggccg tgcggcgaaa tccactattg ccgatctggt ttatgccatt ctgacgtcta
     7441 acccgaaaat ctccacagat aatgtaagtc tgttcgataa agcgaaacat gcaaacgtac
     7501 tggagagcgc ggcaatggac gtggcatcgc tggataaagc ccgccagttg atgcgcgttc
     7561 agaaagaggg tgagcgtcat ctgaatattc gtcctgcgtt cgtactggta ccgacggcga
     7621 tggagtctgt tgctaaccag gtcattcgct cctcaagtgt caagggggct gacattaacg
     7681 ccggtattat taacccggtg aaagattttg cgaccgttat tgcagagcct cgtcttgatg
     7741 ataacagcca gaccaccttc tacctggctg cgtccaaagg ctccgatacg attgaagtgg
     7801 cttatctcaa cggtgtggat acgccatata ttgatcagat ggagggcttc agtgtggatg
     7861 gcgtgacaac gaaagtgcgt attgacgctg gtgttgcgcc agttgatcac cgcggcctgg
     7921 tgaaatgtac ggcgtaaacg tcgcagacaa caactctgat ggcccgtaag ggcttttttt
     7981 gtacctgaaa tcagcccctg aacggggctg tgcggagaca gttatggcaa agaattttgt
     8041 agaagaagga aaaacggtgg cgattgttgc cagtgcagcc atcagcagcg gagatctggt
     8101 gcaggtgggg gatgtttttg cggtggcgct gaccgatatt ccacagggag aaacaggcga
     8161 cggcataacc gaaggtgtgt ttatgctgcc taagctgaaa acggatgaca tgaaaacggg
     8221 taagaaggtt tatctgaagt caggaaaagt tcagctgact aacagcggct ctgagccgct
     8281 ggtcggggtt gtctgggcag atgccggaac cagtgcagaa gaagtgccgg taaaactcaa
     8341 tgtctgatcc cttttcccgg ctggcagcgc gtatggatgc gatcacggtc agaaagatgg
     8401 gaaagacagc ctcgattaat gatgtcgata tgattgtgat cccgggagaa acactggcag
     8461 agctgaatgc tctgtccgga cctgcggtct ctctggtggt gttttcttcg ggataccgcc
     8521 cacggcgcgg ggatcgcgtt gtttatgacg gacaacaatg gacggtcaca cggcatgaac
     8581 gttttaacgg taagccaatg atctttattg agtaaagagg tgtgggatga aggggcttga
     8641 gaatgccatc cgcaatctga acagccttga tacccgtatg gtgccacagg ccagcgcatg
     8701 ggcgataaac cgtgtggcac agaaagcggt ctcggttgcc acccggcagg ttgccgggaa
     8761 taccgttgcg ggagataacc aggtgaaagg gatccccctg aaactggtac gtcagcgtgt
     8821 ccgggtgttt aaagccagtc cgtcaggaaa aatgacggtc aggatccgcg ttaaccgggg
     8881 caatctgccc gccattaagc tggggacagc ccgggtcaga ctggcccggc gtggtggaaa
     8941 actgcagtac cgtggcagtg tgctgaaggt gggtaaatat cttttccggg atgcgtttat
     9001 tcagcaactg gcgaatggtc gctggcatgt gatgcgacgt attgatggca aaaatcgtta
     9061 ccccattgat gtggtgaaaa tcccgctgtc cggatcgctg acacaggcat ttgaagatgc
     9121 ccgcgaccac atcattgctg cggaaatgcc gaaacagctg gggtatgcac taaaacaaca
     9181 actgaggtta tggctgaccc gatgaaccga catacacaaa tccgccaggc cgtactggca
     9241 cgccttcggg aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt
     9301 gatgcgcagg aactgcctgc cgtggcggtg tggctgagtg atgctcagta caccggaaaa
     9361 atgacggatg aagatgactg gcaggctgtt ctgcatattg ccgtcttcat ccgggcacag
     9421 gcaccggatt cagagctgga tatgtggatg gagagcacca ttttccctgc cctgaatgat
     9481 ataccggcac tttccggact catcgacacc ctgatcccac tcggttttaa ctatcaacgt
     9541 gataatgaga tggccacctg ggcgatggcg gaaatcacgt accagatcac gtacacgaat
     9601 taaggaggtg gtaatgacca caccaaatcc actggcaaag acgaaaggtg cggggacgac
     9661 gttctggatg tataccggca acggcgatgc gtttgcgaac cctttgtcgg acactgactg
     9721 gctgcgtctt gcgatggtga aggatctgca acctggcgaa atgaccgctg atgcagaaga
     9781 tgacacttat ctcgatgatg aagatgcaga ctggaaaacg acaacccagg ggcagaaatc
     9841 cgttggtgat acttcggcga cgctggcctg gcgtccgggt gacagcgggc agaaaaaact
     9901 ggttcagttg ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc ccaacggcac
     9961 tgttgatgtt ttccgtggct ggctgagctc actgggtaaa accattgcct caaaagacgt
    10021 gatgacccgc actgtgaaaa tcagcggtgt ggggcgtccg tatctggcag aggaaggcac
    10081 tgaaaccgtg agcgttaccg ggctgacagt atcaccggcg tctgccagtg taaaagtggg
    10141 agcaaccacc acgctgacct ttacagtaaa acctgacgga gccagtgaca aagcgatcag
    10201 tgtgcattcg tcagatccac agactgccac ggttaccctg aacggacttg tggccacggt
    10261 aaaaggcgtg aagcagggca gtgtcagcat tgtgggcatg accgctgacg ggaattttgt
    10321 ggctgtgact gcggtgactg tcagcgcagc aggttaacag gacgatactc atcatttgcc
    10381 ccggttattc ggggcttttt tgcaggtgga gaacatgatg tttctgaaac aggacacgtt
    10441 taattatgaa aaacagtccg tggtgctcag tgagctgtcc gggctgcaga gaattgaata
    10501 tctgacgttt gttcagcagc gaacggcaaa gtttgatgcc ggggagggag aactgccgga
    10561 ggctgaacga cagattgctt ttctgcggat ggggatggat atcaatgcct ggctggtttc
    10621 ccgctcactg tggaatgcgg atcagtctaa ggatgtagag acgctttgcg catccgttat
    10681 tacaacatgg tcgtatgatg ccctgggtgc gggggcggag atggttctgt cgctgagcgg
    10741 tatgggggcc attgataatg ccgtggatga tgagcatgag gcgctgacgc cggaaaagtc
    10801 ctgacgcggg aaatgcagtt tgtcatgcgg cttgcccggg agttccggcg ggcagactgg
    10861 cggcggatgc tgtcggaaat gtcggccact gagcttggtg agtggggcga ttatttccgg
    10921 atgcagagct tcagtgatgt gtggatggat gcgcagtttg cctcgctgaa ggcattgatc
    10981 gtgagaatgg tgtccggcag cagtgatgct gcggtggctg atttcagcct tttaccggaa
    11041 gagaacggga taccggagcg aacggacgaa gaactgatgc atcttgggga aggtatttcc
    11101 ggaggtgtgc gttatggacc agatagccaa cctggtcatt gatttgggga ttgatgcggc
    11161 agagtttaaa aatgaaattc cccgtatcaa aaaccttctg aatggtgcag ccagcgatgc
    11221 agaacggtct tctgcccgta tgcagcgttt tatggagcgt cagactcagg ccgcccggca
    11281 gacaacgcag gcagcttctt cggctgcaac agccgcatcc gtccatgcgc agacggtgga
    11341 gaagaacgca caggctcatg aacgcatggc ccgcgaggtg gagaaaaccc gccagcgcat
    11401 ggaggcactg agccagaaaa tgcgcgagga acaggcgcag gccatggctc tggcggaggc
    11461 tcaggataaa gcggctgctg cgttttatcg tcagattgac agtgtgaaac aggccagtgc
    11521 ggggctgcag gaattacagc gtattcagca gcagatccga caggccagaa acagtggcgg
    11581 gattggtcag caggattatc tggcgctgat ttctgaggtt actgcgaaaa cccgtgttct
    11641 tacacaggct gaggaagagg ctacccgaca gaaagtggcg tttatccgtc agcttaaaga
    11701 gcaggcaacc cgcctgaatc tttcatcttc tgagttgctt cgtgctaagg cagcccagct
    11761 gggggtaagc agtgctgcag aagtgtatat ccgcaaaatg gagcaggcag gaaaagccac
    11821 gcattcgctg ggtctgaaaa gtgcagcagc ccgccaggag ataggcgttc tgataggtga
    11881 actggcccgc ggcaatttag gggcgctgag gggatccggg ataacgctgg ctaaccgtgc
    11941 cggatggata gacacactga tgtcaccgaa aggcatgatg cttggcgggg ttattggcgg
    12001 tattgccgcg gccgtctatg gtctgggtaa agcctggtat gacggtcaga aggaggggga
    12061 agaatttaac cgccagttgt cgctgacggg gcattatgcc ggagtcactg ccgggcagct
    12121 gtggacgctc agtcgtgcta tttccgggaa tggtatcacg caacatgctg cagccggtgc
    12181 gctggctcag gtggtgggga gtggtgcatt tcgtggaaac gatatcggta tggtggcgag
    12241 agttgccgca cagatggagc gatcggttgg ccagtcggtc agcgatacca taaatcagtt
    12301 taagcggctg aaggatgatc ctgtaaatgc cgcgaaggct ctggacaatg agctgcattt
    12361 tcttactgcc actcagcttg agcagatacg cgtccttggg gagcaggggc ggtccagtga
    12421 tgctgcacgg atagccatgt ctgcactggc agaggaaacc ggtcggcgta ctgcggatat
    12481 tgataataac ctcaatgcgc ttggcagtac gctgaagtat ctgtctgatt tgtggagtcg
    12541 tttctgggat gcggccatga atattggtcg tgaagactcg ctggatgaac agattgccgc
    12601 tttacaggag aaagtgtcgc gggcgaaaag actcccctgg acggcatcat cttctcaggt
    12661 tgaatacgat cagcagcgtc ttaacgatct tcaggagaaa aaacgccaga aggatttgca
    12721 ggatgcaaaa gagcaggcag agcggaatta tcaggagcaa cagaaacgcc gtaatgctga
    12781 aaatgctgca ctgaaccgga tgaatgaaac ggaagcagca cgacatcagc gtgaaattgc
    12841 gcgtattaat gccatgcagt acgccgatca ggctgtcagg gatgcggcga tacaacgtga
    12901 aaatgaacgt tacgagaaag ccctggcatc cggtaagaaa aaaacacgcg aaacccgtaa
    12961 tgatgaggcc acccggttat tgctgcagta cagtcagcaa caggcacagg tggaaggaca
    13021 gattgctgct gccagacagt cagcaggcat tgccacggaa aggatgacag aagcgcataa
    13081 acagcttctg gctctgcagc agcgcatcag cgacctggac gggaaaaaac tgacggcaga
    13141 tgaaaagagt gtgctggccc gtaaagatga actgattcag gcactgacgc tgctggatgt
    13201 aaaacagcag gagcttcaga aacagacggc actcaacgag ctgaagaaaa aaacaattca
    13261 gctgaccagt caactggctg aagaagagcg cgctcagcgt cagcaacatg acctggatat
    13321 cgccacggtg ggtatgggtg atcagcagcg acagcgatat caggtacaac tgagtcttcg
    13381 ccagaaatac cagcaacagc tggagcagtt gaggcgggat agtgagcaga aagggacata
    13441 taacacggat gactacagaa aggccgagca ggcgctgacg gagagcctga accgacaact
    13501 gaatgagaat cgccgttact ggcaacagct tgaagttgtg cagggtaact ggaaaaacgg
    13561 agtcctgcgt gcatttcagg attttaccgt ggatgcagat aatacggcag aaacagcaga
    13621 acaggtgttc tcgtcagcct tcagcaacat gggaaatggc ctggcaactt ttgtcactac
    13681 cggcaaactc aatttcaaat ccttcacctc ttctgtgctg tcagatatgg cgaaaatcct
    13741 ggcgcaggca accatgatga aatcgataaa agggattggc agtgtactgg gatttgatct
    13801 cagcagcctt tccctgaatg ccaatggggg gatttatcag tctgctgatt tgagtcgtta
    13861 cagtggcacg gtggttaacc gtccgacgtt ttttgctttt gcaaaaggcg cgggtgtgat
    13921 gggggaagcg ggacctgaag ccattctgcc attgcgtcgt ggtgctgacg gtaagctggg
    13981 ggttgtggcg gatattgggg ggtcaggtat ggcgatgttt tccccgcagt acaacatcga
    14041 gatcaataac gatggcacga acgggcagat aggtccggct gccctgaagg cggtttatga
    14101 cctcgggaaa aaagcggcag cggactttat gcaacagcag gcccgtgatg gtggtcggtt
    14161 aagtggagca tatcggtaat ggagacgttt cactggaaag tgcgcccgga tatgaatgtg
    14221 gtatcagagc cgaaagtggt gacagtgaag ctgggcgatg gttatgaaca gcgtcgtgcg
    14281 gcgggactga ataaccagtt gtcgacttac agcgtgacga tacgtgttcg taaatgtgac
    14341 cacccatctt taaaagcctt tctggaacgg cacggtggcg tccgtgcatt tcagtggacg
    14401 ccaccttatg actggaaacc gatcagggtg gtttgtcgta aatggtcggc aagcgtgggg
    14461 gcgctgtggg taaccataac ggcagatttt gaacaggtcg tggcatagga ggctctgatg
    14521 caggatattc cacaggaaac acatcatgag acgacacgcc tcactcagtc agcccaggtg
    14581 gtgctctggg aaatcgatct gacagaggtc ggtggtgaac gttatttttt ctgtaatgag
    14641 cagaacgaaa aaggtgagcc ggttatctgg caggggcggc agtatcaggt ataccccatt
    14701 caggggacgg gatttgaact gaacggcaag ggcagtgctg cccgtccgac actgacggtt
    14761 tctaacctgt acggtatggt caccgggatg gcggaagatc tgcagagtct ggtcggcgga
    14821 acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga
    14881 aacagtgacg ccgatccgga gcaggaggtg atcagccgct ggcgcattga gcagtgcagc
    14941 gaactgagcg cggtgagtgc ctcttttgta ctgtccacgc cgacggaaac ggacggcgct
    15001 gtttttccgg gacgtatcat gctggccaac acctgcacct ggacctatcg cggtgatgag
    15061 tgcggttatc acggtccggc agtcgcggat gaatatgacc agccgacgtc cgatatcacg
    15121 aaggataaat gcagcaaatg cctgagcggc tgtaagtttc gcaataacgt cggcaacttt
    15181 ggcggcttcc tttccattaa caaactttcg cagtaatccc atgacagaga cagaatcagc
    15241 gattctggcg cacgcccggc gatgtgcgcc agcggagtcg tgcggcttcg tggtgagagc
    15301 gccggagggg gaaagatatt ttccctgcgt gaatatttcc ggtgagccgg aggattattt
    15361 ccggatggct ccggaggact ggctgcaggc agagatgcag ggtgagattg tggcactggt
    15421 ccacagtcat cccggtggtc tgccctggct gagtgaggct gaccggcggc tgcaggtgca
    15481 gagtgatttg ccgtggtggc tggtctgccg gggggcgatt cacaagttcc gctgtgtgcc
    15541 gcatctcacc gggcggcgct ttgagcacgg ggtgacggac tgttacacgc tgttccggga
    15601 tgcttatcat ctggcgggga ttgagatgcc ggattttcat cgcggggatg actggtggcg
    15661 taacggtcag aatctctatc tggataatct ggaggccaca gggctgtatc aggtggcgtt
    15721 gtcatcagca caaccgggcg atgtgctgct gtgctgtttt ggttcatcgg tgccgaatca
    15781 tgccgccatt tactgcggcg acggtgagct gctgcaccat attcctgaac aactgagtaa
    15841 acgagagagg tataccgaca aatggcagcg acgcacacac tccctctggc gtcaccgggc
    15901 atggcacgca tctgccttta cggggattta caacgatttg gccgccgcat cgaccttcga
    15961 gtgaaaacgg ggtctgaagc catccgggcg ctggccatgc agatcccggc gtttcgtcag
    16021 aaactgagcg acggctggta ccaggtacgc attgccgggc gtgatgcagg tgaaaccgaa
    16081 ttgtctgccc gtcttaatga gccgctggca aatggtgccg tgatccacat cgtgccgcgt
    16141 ctggcgggag ctaaaagtgg cggtgtgttt caggtggtgc tgggggcggc gctgattgcg
    16201 gtggcatggt ggaaccctgt gggctggctg ggtgccgcgg ctgtatcggg catgtatgcg
    16261 gcaggggcca gtatgatcct gggcggagtg gcgcagatgc tggcaccgaa agccaggacg
    16321 cccacggcag caagtacaga taacggcaaa cagaacacct atttctcctc actggataac
    16381 atggttgccc agggcaatgt tctgcccgtt ctgtacggtg aaatgcgcgt ggggtcgcgg
    16441 gtggtctctc aggagatcag cacggcagac gaaggggatg gtggtcaggt tgtagtgatt
    16501 ggtcgctgat gaaaaacgtt ttatgtgaaa ccgcctccgg gcggttttgt cgtttatgga
    16561 gcgttaggaa tgggtaaagg cagcagtaag gggcatactc cgcgcgaagc gaaggacaac
    16621 ctgaagtcca cgcagttgct gagtgtgatc gatgccatca gcgaagggcc gattgaaggt
    16681 ccggtggatg gattaaaaag cgtgctgctg aacagtacgc cagtgctgga cactgagggg
    16741 aataccaaca tctccggcgt cacggtggtg ttccgggccg gtgagcagga gcagacaccg
    16801 ccggagggat ttgaatcctc cggatccgag acggtgctgg ggacggaagt gaaatacgac
    16861 acgccgatca cccggaccat cacgtcggca aacatcgacc gtctgcgctt taccttcggt
    16921 gtgcaggcac tggtggaaac cacctcaaag ggggacagga atccatcgga agtccgcctg
    16981 ctggttcaga tacaacgtaa cggtggctgg gtgacggaaa aagacatcac cattaaaggc
    17041 aaaaccacct cgcagtactt ggcctctgtg gtggtggata acctgccgcc gcgcccgttc
    17101 agtatccgga tgcgcaggat gacgccggac agcaccacag accagctgca gaacaaaacg
    17161 ctctggtcgt catacaccga aatcatcgat gtgaaacagt gctacccgaa cacggcactg
    17221 gtcggcgtac aggtggattc ggagcagttc ggcagccagc aggtgagccg taattatcat
    17281 ctgcgcgggc gtattctgca ggtgccgtcg aattataacc cgcagacgcg acaatacagc
    17341 ggtatctggg acggaacgtt aaaaccggca tacagcaaca acatggcctg gtgtctgtgg
    17401 gatatgctta cccatccgcg ctacggcatg gggaaacgtc ttggtgcggc ggatgtggat
    17461 aaatgggcgc tgtatgtcat cggccagaat tgcgaccagt cagtgccgga cggctttggc
    17521 ggcacggagc cgcgcatcac ctgtaatgcg tacctgacca cacagcgcaa ggcgtgggac
    17581 gttctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg gcagacgctg
    17641 acgttcgtgc agaaccgacc gtcggataag gcgtggacct ataaccgcag taatgtggtg
    17701 atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctgaa
//
LOCUS       DAZKGS010000089        17740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKGS010000089 DAZKGS010000000
VERSION     DAZKGS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..787)
                     /gene="rlmB"
                     /locus_tag="ACHOQY_003001"
     CDS             complement(56..787)
                     /gene="rlmB"
                     /locus_tag="ACHOQY_003001"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9679059.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(967..3408)
                     /gene="rnr"
                     /locus_tag="ACHOQY_003002"
     CDS             complement(967..3408)
                     /gene="rnr"
                     /locus_tag="ACHOQY_003002"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9679060.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3447..3872)
                     /gene="nsrR"
                     /locus_tag="ACHOQY_003003"
     CDS             complement(3447..3872)
                     /gene="nsrR"
                     /locus_tag="ACHOQY_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9679061.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4077..5375)
                     /gene="purA"
                     /locus_tag="ACHOQY_003004"
     CDS             complement(4077..5375)
                     /gene="purA"
                     /locus_tag="ACHOQY_003004"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9679062.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5479..5676)
                     /gene="yjeT"
                     /locus_tag="ACHOQY_003005"
     CDS             complement(5479..5676)
                     /gene="yjeT"
                     /locus_tag="ACHOQY_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9679063.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5758..6762)
                     /gene="hflC"
                     /locus_tag="ACHOQY_003006"
     CDS             complement(5758..6762)
                     /gene="hflC"
                     /locus_tag="ACHOQY_003006"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9679064.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6765..8024)
                     /gene="hflK"
                     /locus_tag="ACHOQY_003007"
     CDS             complement(6765..8024)
                     /gene="hflK"
                     /locus_tag="ACHOQY_003007"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9679065.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8110..9390)
                     /gene="hflX"
                     /locus_tag="ACHOQY_003008"
     CDS             complement(8110..9390)
                     /gene="hflX"
                     /locus_tag="ACHOQY_003008"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9679066.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9467..9775)
                     /gene="hfq"
                     /locus_tag="ACHOQY_003009"
     CDS             complement(9467..9775)
                     /gene="hfq"
                     /locus_tag="ACHOQY_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9679067.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9861..10811)
                     /gene="miaA"
                     /locus_tag="ACHOQY_003010"
     CDS             complement(9861..10811)
                     /gene="miaA"
                     /locus_tag="ACHOQY_003010"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9679068.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10804..12651)
                     /gene="mutL"
                     /locus_tag="ACHOQY_003011"
     CDS             complement(10804..12651)
                     /gene="mutL"
                     /locus_tag="ACHOQY_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9679069.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12661..14001)
                     /gene="amiB"
                     /locus_tag="ACHOQY_003012"
     CDS             complement(12661..14001)
                     /gene="amiB"
                     /locus_tag="ACHOQY_003012"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9679070.1"
                     /translation="MMYRIRNWLVATLLLLLCTPVGAATLSDIQVSNGNQQARITLSF
                     IGDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVD
                     LTENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARN
                     PFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKN
                     VTIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPN
                     RSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQ
                     FGHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSE
                     ERLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14020..14481)
                     /gene="tsaE"
                     /locus_tag="ACHOQY_003013"
     CDS             complement(14020..14481)
                     /gene="tsaE"
                     /locus_tag="ACHOQY_003013"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9679071.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14453..16000)
                     /gene="nnr"
                     /locus_tag="ACHOQY_003014"
     CDS             complement(14453..16000)
                     /gene="nnr"
                     /locus_tag="ACHOQY_003014"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9679072.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15999..17138
                     /gene="queG"
                     /locus_tag="ACHOQY_003015"
     CDS             15999..17138
                     /gene="queG"
                     /locus_tag="ACHOQY_003015"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9679073.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17121..17174)
                     /locus_tag="ACHOQY_003016"
     CDS             complement(17121..17174)
                     /locus_tag="ACHOQY_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679074.1"
                     /translation="MRFYFYSQAVDESGVTW"
     gene            complement(17408..17483)
                     /locus_tag="ACHOQY_003017"
     tRNA            complement(17408..17483)
                     /locus_tag="ACHOQY_003017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17448..17450),aa:Gly,seq:gcc)
     gene            complement(17519..17594)
                     /locus_tag="ACHOQY_003018"
     tRNA            complement(17519..17594)
                     /locus_tag="ACHOQY_003018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17559..17561),aa:Gly,seq:gcc)
BASE COUNT         4012 a         5073 c         4447 g         4208 t
ORIGIN      
        1 ctatagacaa aaaaaaacca tccaaatctg gatggctttt cataattcag acgaactagc
       61 tgcgctgacg aaccgcttca aataagcaaa ttccggttgc aaccgaaacg ttcagggaag
      121 aaacacttcc tgccattggg atgctgatca actcatcgca atgttcacgg gtcaggcgac
      181 gcataccttc accttccgcg cccatcacca gcgccaggcg tccggtcatt ttgctctgat
      241 agagtgtatg atccgcctcg cctgccgtac cgacgatcca gatattctct tcctgcaaca
      301 tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc
      361 aggctacttt tttcgccgta gcgttgagct gtgcggagcg atctttcggc acaatcaccg
      421 cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta
      481 caccgtcgag gatcagcagg aacggttgat cgagcgaagc gatcagatcc ggcagatcgt
      541 tttcctgata ctgacgtcct ggcttcacgc gggcgataat gccctgatgc acggcaccgt
      601 cgcttttctc gtcgagatat tggcggtttg ccaactggat aaccacgccc tgagactcaa
      661 gggcgtgaat cagcggtaac agacgtttat cttcacggcc ttttaaaata aagacttcct
      721 gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc gtggatgccg taaatcattt
      781 cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc actacgttta tccggcctac
      841 atgatctctg caatatattg aatttgcgtg attatgtagg ccggataagg cgttcacgcc
      901 gcatccggca tgaacaaagc gcattttgtc agcaatctaa ccctcttctt ttaaagaggg
      961 tattgatcac tctgccactt ttttcttcgc cgcacgcttc gctttggtcg ctgcggctat
     1021 tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg tctttcttcg ccgctttcgg
     1081 cttcgttttt ttctcaccgc ggaaggcgct gtctggctca aagtttacct ttttaccgac
     1141 ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt ttcgctttct cgcgcgccgt
     1201 tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga ctaaagtcga ttttgcgctc
     1261 gtccatatta accgcttcga ctcgaacttc cacgcgatcg cccaggcgat aagtctggcc
     1321 gctggattcc cccatcaggc gttgccctac ctggtcaaag cgatagtagt cattgtccag
     1381 cgaagagaca tggaccagac catcaatgaa caagtcgtcc agacggacga agaagccaaa
     1441 gccagtgacg ctggaaatta cgcctttaaa gacgttacct acctggtcga gcatgaagtc
     1501 acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg gcacgacgtt ccgccatcga
     1561 acagtgctga cccagttgca acatctcttc catcgaataa tggtagccgc cggtttcagt
     1621 ggtgttgccc tgatgcccct gctctttcgc cagcagatat ttaatggcgc ggtgcagcgt
     1681 caggtcagga taacgacgaa tcggcgaagt aaagtgcgca taggactgca atgccagacc
     1741 gaagtgacca cggttttctg gatcgtaaat cgcctgtttc atcgagcgta gcagcatggt
     1801 ttgcagcatt tctgcatcag gacggtcggc aaccgactcc agcagttccg cgtagtcacg
     1861 cggttccggc ttgttaccac ccggcagctc cagccccagc tccgccagca ctgaacggaa
     1921 agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg aacagtgccg gttctttcgc
     1981 tttctcaacg aaacgcgccg ccgagatatt cgccagaatc atgcactctt caattaattt
     2041 gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata cggcgttcag cgttgaaaat
     2101 gaacttcgct tcttcgctct caaatgagat cccaccgcgt tcttcacggg ctttatccag
     2161 cactttatag aggttatgca actcttcgag atgcttaacc agcggggcgt actgctcgcg
     2221 cagatcctga tcgccctgca gaatatgcca gactttggtg taggtcagac gcgcgtgaga
     2281 gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg cctttcgacg aaaccgtcat
     2341 ctcgcacacc atacacaggc ggtctacctg cgggttgagc gaacacaggc cgttagagag
     2401 cacttccggc agcatcggga taacctgcga agggaagtac accgacgtgc cacggttacg
     2461 cgcttctctg tccagcggcg ttggcggacg cacatagtag ctgacgtcgg caatcgcgac
     2521 ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa actgcatcgt caaagtcacg
     2581 ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc agatcaacac ggcccgcttt
     2641 tgcttcttcc ggcacttctt ctttcagccc ggcaacctgt tgctcaacag cctgcggcca
     2701 gatgtacgga atttcatggg tacgcagagc gatatcaacc gccatgccgg tgcccatatt
     2761 gtcgcccagc acttcgacga ttttacccac cgctttggtg cggcgagtcg gacgctgagt
     2821 cagttcgact acgaccacaa agcccatccg cgcgcccatg atctgatcgg gcgggattaa
     2881 gatatcgaag ctcagacggc tatcgtcagg aaccacaaag ccgacgcccg cttcagtaaa
     2941 gtagcgacca acaatctggc tggtttttgg caccagtacg cggacaatac gcgcttcacg
     3001 acgaccttta cggtcagcgc ccagcggctg cgccagcacc tgatcgccat gaatgcaggt
     3061 tttcatctgc tcgctggaga gatacaaatc atctttacgc ccttcaaccc gcagaaagcc
     3121 gtagccatca cggtggccaa taacggtacc tttcaccagg tcgaggcgtt ccggcagcgc
     3181 atagcactga cgacgagtga agaccagttg accatcgcgc tccatcgcgc gcaggcggcg
     3241 acgcaggcct tcaagctgct cttcgccttc aatgtgcagt tctaccgcca gctcatcacg
     3301 gctggccggt ttttcacgtt tggttaaatg ttcgaggata aattcccgac tagggatggg
     3361 attcgcgtat ttttcagctt cgcgttcctg gaaaggatct tgtgacatct cggttcctcc
     3421 gttgtcatct ctgatgaaga ttttcgtcac tccaccagca ataatttata aagcggttga
     3481 ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt ccgtaagaaa actttgcacg
     3541 gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa tgtggcaaaa ctcactgctg
     3601 caattcacca gcgataaggg ctccagctcg cgcaccacat caccaatacg tatcgcactc
     3661 gccggtttac ccaggcgaat gccgccattt tttccacgaa cagcagtcac gtagccggca
     3721 cgactaagtt gattgattat tttgaccata tgattacggg agacgccgta gacgtcagtc
     3781 acttcagaaa tactggtcat ccgcccttct ggcaatgacg ccatgtagat cagcgcacgt
     3841 aatccgtaat cagtgaaact cgttaactgc acatcaacct caaaagggaa atcgggaaaa
     3901 aatacattta tattgatgat aaaccagcca caagctgtgt cgctaattta tttcagttcg
     3961 gggaaggaaa aaagcgagga tttaacactg tgtcggatag cgtaatggct taaccgacac
     4021 agcaatgtat cacactgtta gcccggcagg caaaatatct gccaggcgta ccagaattac
     4081 gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat ccggaccggt agagatgata
     4141 tcgatcggca caccagtcag ctcttcaata cgcttgatgt agttcagcgc cgcctgcggc
     4201 agaccgctac gatctttcac gccgaaggtg gattcagacc agcccggcat ggtttcgtaa
     4261 atcggctcta cacctttcca gtcgtcagct gccagcggag tggtcgtcac ttcacgacca
     4321 tccggcatac ggtaagccac acagagtttc acctctttca ggccatccag aacgtccagt
     4381 ttagtcaggc agaagccaga cagggagttc agctgtaccg cacgacgaac ggcaacggtg
     4441 tccagccagc cggtacgacg acgacgcccc gtagttgcgc cgaattcgtt accctgcttg
     4501 cagaggaact cgccagtttc atcaaacagt tcggtcggga acggacctgc acctacacga
     4561 gttgagtaag ctttgaggat acccagaaca taatcaacat aacgcgggcc caggccggaa
     4621 ccggtcgcca cgccaccagc agtggtgttg gaagaagtta cgtacggata agtaccgtgg
     4681 tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga cgaaatcgcc acgctgacgc
     4741 gcctggtcga gcaggtcaga aacgtcaacc accatagaag tcaggatgtc ggcaacagcc
     4801 atcgtatcat ccagaacttt ctggtaatca accgcttcag ctttgtagta gttaaccaac
     4861 tggaagttgt gatattccat cacttctttc agtttttcag cgaaggtttc tttgtcgaaa
     4921 aggtcgccaa cacgcagacc gcgacgtgct actttatctt cataagcagg cccgatacca
     4981 cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct cacgcgcgtt atccagcgca
     5041 acgtgataat caaggatcag cggacatgct tcagacagca gcagacgctc acgaacgggg
     5101 atgccacggt cttccagttc tttcatctct ttcatcagcg cggccggaga cagcacaaca
     5161 ccgttaccga tgatgctggt tacattctcg cggagaatac ctgatggaat aagatggaga
     5221 acggtttttt caccgttgat tacgagagta tggcctgcgt tgtgaccgcc ctggtagcgt
     5281 acaacatatt tagcccgttc agtcagaaga tcgacgatct tacctttacc ttcgtcaccc
     5341 cattgggtgc ccagtacgac gacgttgtta cccatttttc aaaatcaccg tttgcttaaa
     5401 aatggattct accatcgctt tttcagagtt acagcacttt ttgcactcaa aaatgacctc
     5461 gatcagtctg ttttttgttc agccaatcgt tttcctcaac atgtagtaga ccacaacgcc
     5521 cgcaaccaca agtccaccgc caaaacgacg taaaatatta tcgggcaaat tggtcatcgc
     5581 agagatcatc ttcttccatg ccttcgggta aagcatcggc cctaaacctt ccagtaccaa
     5641 aaccagggca agcgccagcc agattgtcga attcattcat tatccttata gaaaaagaaa
     5701 accaccgaca tccctgagga tgcggtggct ttattgacct gtaccgcagt cgttatatta
     5761 acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa tcgctatccg ggctcatgac
     5821 catcacgtcc tgattgccag agaagctgtt ctcataagca cgcaggctac ggatgaatgc
     5881 gtagaagtcc ggatctttac tgaatgcatc agcaaacagt ttggctgctt cggcatcgcc
     5941 ttcaccacgc atgatgcggc cctgacgctc agcttctgcc agcgttctgg tcacttcata
     6001 gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct tgtgaacggt gacgacgcgc
     6061 taccgcttca cgctcggcgc gcatacggtt gtagatcgct tcagacactt cggtcggcag
     6121 gttgatctgc ttgatacgca catcgacaac ttcaataccc agcgccgcca tactgttcgg
     6181 gttgatgacc ggaactttgc ccttcgtctc agccgttacg cgctctgccg cttcggcaat
     6241 ggcgttatct gctgccgggg tagtaacttc atcttctgta cccgcagaac cggagttcag
     6301 cgcgtcacgt acttcgaggg tcagacgacc acgggaatca gtgacgatat ctttcacgtc
     6361 caggcgacca atttcagaac gcagacggtc agagaactta cgtttcaaca gcacttccgc
     6421 ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg ctgaaatcgc tgatgcgcca
     6481 tttgatgtaa gaatcgacga tcaggtcttt cttctctttg gtcacaaagc ggtcggcctg
     6541 gttgtccatg gtctgaatac gtgcgtcgag cattttcacc gtttcaatga acggtatctt
     6601 gaaatgcaga cccggctcat aaaccagagg tttgttgtca tcgtcacgca gtaccttacc
     6661 aaaacgcagc gtaataccgc gctcaccttc tttgacgaca aagacagaca tgtaaagcac
     6721 taccagcacg atgatgataa tcgcgataac tgacttacgc atcgttattc cccctgacgc
     6781 tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca taatatcgcc ctgactggtg
     6841 gacgacgtgt tgcttgctcc actggttgtg gaggaagagg ctggcggcag acgcagcaga
     6901 ttgctggcac cgttatcgct cttcgccgca ggggcgttac cacctttcag catctggtct
     6961 aacggcagaa ccatcaggtt tccacctttg tcgttaacca gcactttgcg ggtgttgccc
     7021 aacacttttt ccatcgtctc gatatacaga cgctcgcgag taatttccgg cgcggcttta
     7081 tattccggca gaagtttagc aaagcgcgcc acttcaccct gagcttccag gatggtctgg
     7141 gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct gaccgttcgc acgcggctga
     7201 acttcgttgg tatacgcttc tgcttcacga atgtattgct gttcgttttc acgcgcggca
     7261 atcgcatcgt caaacgccgc ttttacttct tccggcggac gagcagcctg gaagttgacg
     7321 tccagcagcg tgatacccat gtcatacgga cgaatcgtct cttccagttc gcgctgagta
     7381 tcgctacgaa tcacggtacg accttccgtc agaatgcggt ccatggtgta tttaccgata
     7441 actccacgca gggcgctgtc ggtagcctga cgcaggctgt catccgggct ggtcacgcta
     7501 tacagatatt tttccggatt ggtgacgcgg tactgcacgt tcatctcaac gcgcactacg
     7561 ttctcgtccg acgtcagcat cacaccagaa gcggccagtt cacgcacggc ttccacgttc
     7621 accggtttga cttcgtcgat aaacgtcggt ttccagttca gacccggctc aaccagatgg
     7681 ctgaatttac caaagcgtgt taccacgccg cgttcggctt ctttaatggt atagaaacca
     7741 ctggccgccc agataatgac aatcgctgcc gctgcgatgg taacgacacg accgccaagc
     7801 tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc cggtgccttt accgccgccc
     7861 agaccaccga gctttttgct cagtttgcgg aagatatcat ctaaatcagg tggcccttga
     7921 tcgcgaccgc ctttgtttcc atttccctca gagttgccgc caggtttgct gcttccccac
     7981 gggtcgcggt cttgtccgtt attaccgggc tgattccacg ccatgtttgt gctccatatt
     8041 tgttatgcgg tgatccctgt tgtctttcga cgccagagga tatgactcca cgcttcagac
     8101 gctacgccgt tagatcaggt aatcgatcaa cgccggttct tgtttacaga ggcgacgcca
     8161 gtcaacgatc ggcatacgaa cttgcagact tacgctgccg tcctcctcca tccactcttt
     8221 ttctattgcc tgaagctgat aaaaacgact tctcagacgc ccttcctgcg gtggcagacg
     8281 caatgtatgc tgcgccacct cgccggaaag ccgccccgtc aaagcctgaa aaagctgtgg
     8341 tatccccgct ccggtctgtg cggaaagcca gacacggatc ggtttgttct cttcgtcccg
     8401 atcaatacgc ggttcgaaat cttccagcat atcgatcttg ttcatcacca gcagggttgg
     8461 gatctcgtga gcgtcgatct cttcaagaac cgtattcacc gcttcgatgt tttcttgtac
     8521 acgcacatcc gccgcatcaa tgacgtgcag cagtaatgtg gcttgccgcg tctcttgtaa
     8581 cgtggcttta aatgccgcca ccagatcgtg cggcaggtgg cgaataaacc ctacggtatc
     8641 tgcaagtacg gtttcaccga catctgcaac gtcaatacgc cgcaacgtcg ggtcgagggt
     8701 ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg atgcgattga aaagggtaga
     8761 tttaccggcg ttggtatatc ccaccagcga aacagtagga acgtcggctt tgatacgcga
     8821 ttgccgcccc tgctcacgct gcttttcaac tctttccagg cgtgactgta tctgcacgat
     8881 gcgattacgc aacaaacgac ggtcggtttc gagctgggtt tcacccggac cacgcaaacc
     8941 tatcccgcct ttctgtcttt caaggtgggt ccagccacgc accaggcgcg tagccagatg
     9001 gcgcagctgc gccagctcaa cctgcaactt accctcatgg gtacgcgcac gttgggcgaa
     9061 aatatctaaa ataaggccgg tgcggtcgat aacacgacac tcgcacaaac gctccaggtt
     9121 acgctcttgc gccgggctca gggcatggtc aaaaagaacg accgaagcac ccgtcgcttt
     9181 gacagcttcc gcaatttcaa ctgctttacc ttcacctaca aaatactttg ggtgcggcgc
     9241 tttacggcta ccggtaatca cctgcaatgc ttcgacaccg gcggaagaga ccagagattc
     9301 aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa tagatgtgta ccagtaccgc
     9361 ctgctcacca gcatcataac ggtcaaacaa gcgtataacc ctctaaatag atcagcgggg
     9421 aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc gaaaccttat tcggtttctt
     9481 cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc atgatggtag ttactgctgg
     9541 taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg agacgggaca acagtagaaa
     9601 tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa caggatcacg aattgatcaa
     9661 aagactcgat ttgcccttgc agcttaatac cattcaccaa ataaatagaa actggaacac
     9721 gttcccgacg cagtgcgttc aggaacggat cttgtaaaga ttgcccctta gccattctct
     9781 cttttcctta tatgcttatt tgtactttga acctttcgat tctgaaaaaa ttgcgcacga
     9841 tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa caacctgtaa tacttcgtcg
     9901 cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc cttcccaacc acgcagccag
     9961 gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac ctcgataaac catttcatcg
    10021 tatgagattt cgccttcaag gtaagaccac atctggcgat aacccacgca acgaatggaa
    10081 ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc ggacttctgc ttcaaaacct
    10141 gaagccaaca tctgatgaaa acgctgctca atgcgttgat ggagcagttc acggctcgcc
    10201 ggggcgatgg cgaactgatg cacctgatac ggtagagcgt ctcctgacgt ttgcgtcagt
    10261 tccgttaaag ttttacccga aatgaaaaaa acttccagtg cccgggaaag cctttgtgga
    10321 tcatttggat gaatccttgc tgccgcaacc ggatctacct cctgaagttg acgatgcaat
    10381 gactcccaac cttgctctac cgcctgttgc tcaattctgg cccgcacttc cgggtctgcc
    10441 gacggtagcg gcgacaaccc ttccagcaat gccttgaaat acaacatcgt accgcccact
    10501 aacagtggga tccgccccgc cgcggtgata tcggccattt ccgccagcgc atcgcggcga
    10561 aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat ccagcaatcg gtgcggcgcg
    10621 gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat ccatcccttt gtaaataagg
    10681 gcagaatcaa cgcttatcaa ctctactggt aaaattttac gcagctcaat ggctaacgcc
    10741 gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct taggcaggct cgccttactg
    10801 atatcactca tctttcaggg cttttatcgc cggatgtaaa tcaacagatt gtaacagacc
    10861 acccggcggc gttttcacaa gttgcggaca taaccgttcc acgtccgcca gcagggttat
    10921 ggcctgtgcc attgaccact gcgcatgttc gctcatcaga tttcgtgcaa tccactgcgc
    10981 aatattgcca ggttcgaata cggactgctt cgccaggtag cctatcagtt caggaatcaa
    11041 gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc ctgatggtca catgctgtgc
    11101 atctgactgg aaatcaatac ccaattccgc cagggcagac tgcgcttttt ctaatgccga
    11161 tttttcttcg ccagaaactt ttagccgcaa cggaatcagc aacggctggg cgcaaactgg
    11221 cgcttcaccc ggcgtcagtt gcgcctgacg cagccaacgt tctgccactg gcaaggctaa
    11281 aagtgaaatg ttgccatcgc gctccagcaa cgcacagtcg gaatggacga tagtcagtac
    11341 ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc tgcggttccg gcgcttttgg
    11401 tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac acttcacctt gctgtttctg
    11461 gtagcctggc tgcgcattcg gccagggggc agccggacga ctgcctgagg cgggcgcagg
    11521 agagtagcgc ggagctaccg gctcacgagc tgccggttct gcaaagtgat tgcgccccgc
    11581 cgccacgcgg ttttccggaa tgggacgcgg tgcaggttgg ggttcatcgt ccagcggtag
    11641 cggcgtttcc agttgctgtt gtagcacgct cagcacgccc tgatagataa aatcatgcac
    11701 cagacgcgac tgatggaaac gcacttcgtg tttggcgggg tgtacgttga cgtccacctg
    11761 atgcgggtcg atctccagat acaacacaaa cgccggttgt tgatcggccc ccagtttgtc
    11821 ttcgcaggcc tggcggatcg catgattgat caggcgatcg cgcatcatgc gaccattcac
    11881 gtagcaatac tgaatttccg ccagtgcggg cgtagtgtga tttggatctg ccacccagcc
    11941 gcgcagcgtg agatcgccgt gttgccattc aatcgccagc gcttgttcga gaaaagcggt
    12001 gccacaaatc gcgcctaagc gccgttcttt ttgcccgcct tccggcactg cgcggtactg
    12061 acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg tcgaaacgcg ccagcgcaat
    12121 gcggcggatg atttcatcaa tatggctaaa ttcggttttc tcggtgcgca ggaatttgcg
    12181 ccgcgccggg gtgttgtaga acagatccag cacctccagc gtcgtcccca caggatgcgc
    12241 cgccggtttt accgtcacgt tcatatcgcg cccttcggca taggcctgcc aggcttcctg
    12301 ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc gaactgatac tcgccagcgc
    12361 ctcaccgcga aagcccaggc tgataatggc ttcgagatcg tccagagagg cgattttact
    12421 ggtggcatga cgagccagcg ccagcgccag ctcatctttt ttgataccgc agccgttatc
    12481 acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca atatcgatac gcgtcgcacc
    12541 tgcatcgagg ctgttttcca ccagttcttt gactaccgac gcaggtcgct cgaccacctc
    12601 acctgcggca atctggttcg ccagttgtgg cggtaagacc tgaattggca tcaatcgtcc
    12661 ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg gcagtttgtg ccgttgcccc
    12721 ctgcggcgca gattgcatcg gatgcgcaag gaaataattg cgcaggcctt tgtaaatggc
    12781 ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa cgttcttcgc tgttgttgct
    12841 gataaaaccg gtttcgacca gtactgacgg gatatccggc gaacgcagaa cgccgagact
    12901 cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt tgcaactgac tgatcatact
    12961 ggtcgctaca tcatacccta cccgttggga atgaccgaac tgtaaatcca gcaccgcctg
    13021 gcttaaatag gggtcagact gactgttcgc cagcacatca cccgccccac ccagcagctc
    13081 cgactgtttc tcgtgctgct ccagccagct ggccatttcg ctgttggcgc gacggttaga
    13141 gagcacccat acggaagcgc cagtcgcact gcggttcggt gcggcatcag cgtgaatcga
    13201 cacgaggaaa ttggcgtttt gcttacgtgc cacatcactg cgccccatca ccgagataaa
    13261 gtaatccccg tcacgggtta aaacgccctt aaacatcgga tcgtcattga gcaaagtacg
    13321 caatttacgc gcgatggcga tggtgacatt tttctcccgc gtaccaccgg ggccgatagc
    13381 gccagggtcc tgaccgccgt gtccggcatc aatagcgatg ataattttat cgccagtgtt
    13441 agccgtcgcg cgcgctgccg gacgcgttac cgtattactg ctgataacac ccgtagtgcg
    13501 gttactttcc gttttaaacg gattgcgcgc cggttcgctg acgcgcggtg cgacaaccgc
    13561 aggcgtttca acgcgtttcg caaccacggg cggcggagga ggaggtggcg gcgcatcggc
    13621 gttaatcgta aagacgacag tgtaattgct gccattctgc cgcttcaccg cttcggtttt
    13681 accgttttcg gtaagatcga ccaccagccg tagcgtttgt gcatctttag gcgttccaga
    13741 gcgaatcgcc ttcaccagat tattgccgct gaacaacaac ggcagtccct gaatcacgcc
    13801 cgtttgtttg atatcgagcg ccacggtgcg tttgctttga tggctaaacg cataatcagg
    13861 atcgccaata aaactcaacg ttatccgcgc ctgttgatta ccgttagaaa cctgaatatc
    13921 agagagcgtc gcggcaccca ccggcgtgca cagcagcagc agcagcgtcg ctaccaacca
    13981 atttctgatg cgatacatca tcccgccacc tttcaaaggt taaccggcta aacgcgccag
    14041 caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata
    14101 atcaatgtgt atttcgacat ccgggtcagg aagaacacct gtaccttgtt gtggccactc
    14161 caccaggcag atggcatcgt tggcaaaata atcgcggatc cccataaact ccagctcctc
    14221 gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcgtataggg
    14281 ttcgaccagc gtataagtgg ggcttttgac attaccctga tgacccagag cttgtaaaaa
    14341 gccccggcta aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc
    14401 gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag
    14461 cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta
    14521 gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt
    14581 cagctgcagc accgtgcgcg acacagcccg cacaggcggc atcatacggc gacagttttt
    14641 gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgccg ctcgccatgc
    14701 ctgcatttcc ggcatcaata atgcctaaag cgtcaggatg ggcggcgacc acggttccgg
    14761 cacctttcag caccgctacg ccgccataac gttgtaccag acgtttggcg caatgtaagc
    14821 ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg
    14881 gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg
    14941 catcccacaa catcggtttg cgaaaattct caactttttg cagtgctttt ttcccccact
    15001 cttgctggcc cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa
    15061 gagagtccat cgtcagttca tgcaccatca attccggtcg tgcagtcagc agcggcgcaa
    15121 tgttttcact gcgggtcagt actcggacta aaccagcacc agcacgtagc gccgcttccc
    15181 ccgtcatacg aatagccccc gccgtgccgt gatcgccacc gataattacc agccgcccgt
    15241 gatcgccttt atgcgaagtc gggcgacgcg gttttagcca gtgagaaagt tgttctgccg
    15301 aaaaccgctg aattttcgtc tcctgacctg ccagccaact atccagcccc agtgagtcaa
    15361 aatgcagttg tccggtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa
    15421 taaaagtgat ggtgtgatcg gcgttgatca ctgcgcctgg cgtagcgccg gtttcagcca
    15481 gcaggccgga agggatatca accgccgcaa tcggcgcagg atgggaatta gcgtggtcga
    15541 ttaactggct aatggattcg cggggcgctt gctgcaagcc ggtaccgagc agcgcatcaa
    15601 caatcagatc taccgattcg ggccagacaa tattcgaagc atggatctcg cctcccgcgt
    15661 ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa cggtttgtcg ctctcctggg
    15721 ccaacaacgt gacctcaatg ccgaccgctg tggccagtcg cgcgaccacg tagccatcgc
    15781 cgccgttatt accatgaccg cacagcacca gccagtggcg ggcgtcagga tacgccgaac
    15841 gacacacctg gaatgcggct tcgccagcgc gaagcatcag ctcatagagt gtaagcccca
    15901 gcgcatctgc cgcctcacgt tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg
    15961 gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca
    16021 atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta
    16081 ttaccgatac cgatctcagc gagtccgagc ccaaactgca agcatggctg gacaaacaat
    16141 accacggcga aatggactgg atggcacgtc acggtatgct gcgcgctcgc ccccatgagt
    16201 tattacccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg
    16261 catttgccag cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc
    16321 gtgactatca caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac
    16381 attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctc gagcgcccgt
    16441 tagctgcaaa agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg
    16501 ccggttcttt cttcttttta ggcgaattgc tggtcgatat tccgctgccc gtggatcaac
    16561 cagtcgagga aggatgcggt aaatgcatag cctgtatgac gatttgcccg accggtgcca
    16621 tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaactgg
    16681 aaggggcgat cccggaagag ttgcgaccgt taatgggaaa ccgtatttac ggttgcgatg
    16741 actgccagct tatctgcccg tggaatcgct attcgcaact cactacagaa gacgatttca
    16801 gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga
    16861 agtttttaaa agtcacggaa ggatcggcga ttcgccgtat aggtcacctg cgctggctgc
    16921 gtaatatcgc cgtggcatta ggcaatgccc cctgggatga aacgattctg gcggcgctgg
    16981 agagtcgcaa aggtgagcac ccacttcttg atgagcacat agcgtgggcg atggcgcagc
    17041 aaatcgagag gcgaaatgcg tgcatagtcg aagtgcaatt gccgaaaaaa cagcgtctgg
    17101 ttcgggtgat tgaaaaaggg ttaccacgtg acgcctgatt catccacagc ttgtgaataa
    17161 aaataaaaac gcattgcaat tcaagacgaa taaaatcgtc aagtgatcga ctcgacaaaa
    17221 cgaaatctta atttttaact tatctatcaa taagttaaaa aaatactatt caccgtgcga
    17281 agtttctttg aaattaagac tacagcctga ggctgtggat aactctgttt acaagagttt
    17341 gtcagagaca acaaaaacca gcaataacgc atcgcgtcgc tgtggatatt ttattgagag
    17401 aagaatttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    17461 tctaccaact gagctattcc cgcttgggtg gtctgtgcct tacagcactt tcaaattttg
    17521 gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
    17581 tgagctattc ccgcttaatc ttcgtctttc gaaccgctac tgccttggct gccttcactc
    17641 accttagtca catacttttg taagttccca aggattcgtt cagttgccgc cttgtcgcaa
    17701 ttcgaaatac tgcgattggg tggtctgtgc tttgcagcac
//
LOCUS       DAZKGS010000090        17698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKGS010000090 DAZKGS010000000
VERSION     DAZKGS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(173..514)
                     /locus_tag="ACHOQY_003019"
     CDS             complement(173..514)
                     /locus_tag="ACHOQY_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9679075.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(527..1399)
                     /gene="copD"
                     /locus_tag="ACHOQY_003020"
     CDS             complement(527..1399)
                     /gene="copD"
                     /locus_tag="ACHOQY_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9679076.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(1403..1777)
                     /gene="yobA"
                     /locus_tag="ACHOQY_003021"
     CDS             complement(1403..1777)
                     /gene="yobA"
                     /locus_tag="ACHOQY_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9679077.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            1916..2146
                     /gene="holE"
                     /locus_tag="ACHOQY_003022"
     CDS             1916..2146
                     /gene="holE"
                     /locus_tag="ACHOQY_003022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9679078.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            2248..2904
                     /gene="yobB"
                     /locus_tag="ACHOQY_003023"
     CDS             2248..2904
                     /gene="yobB"
                     /locus_tag="ACHOQY_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9679079.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            2928..3590
                     /gene="exoX"
                     /locus_tag="ACHOQY_003024"
     CDS             2928..3590
                     /gene="exoX"
                     /locus_tag="ACHOQY_003024"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9679080.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3587..5647)
                     /gene="ptrB"
                     /locus_tag="ACHOQY_003025"
     CDS             complement(3587..5647)
                     /gene="ptrB"
                     /locus_tag="ACHOQY_003025"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP9679081.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(5856..6515)
                     /locus_tag="ACHOQY_003026"
     CDS             complement(5856..6515)
                     /locus_tag="ACHOQY_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9679082.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(6842..7198)
                     /gene="yebF"
                     /locus_tag="ACHOQY_003027"
     CDS             complement(6842..7198)
                     /gene="yebF"
                     /locus_tag="ACHOQY_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9679083.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(7265..7555)
                     /gene="yebG"
                     /locus_tag="ACHOQY_003028"
     CDS             complement(7265..7555)
                     /gene="yebG"
                     /locus_tag="ACHOQY_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9679084.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            7689..8867
                     /gene="purT"
                     /locus_tag="ACHOQY_003029"
     CDS             7689..8867
                     /gene="purT"
                     /locus_tag="ACHOQY_003029"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9679085.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(8923..9564)
                     /gene="kdgA"
                     /locus_tag="ACHOQY_003030"
                     /gene_synonym="eda"
     CDS             complement(8923..9564)
                     /gene="kdgA"
                     /locus_tag="ACHOQY_003030"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9679086.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(9601..11412)
                     /gene="edd"
                     /locus_tag="ACHOQY_003031"
     CDS             complement(9601..11412)
                     /gene="edd"
                     /locus_tag="ACHOQY_003031"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9679087.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(11647..13122)
                     /gene="zwf"
                     /locus_tag="ACHOQY_003032"
     CDS             complement(11647..13122)
                     /gene="zwf"
                     /locus_tag="ACHOQY_003032"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9679088.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            13460..14329
                     /gene="hexR"
                     /locus_tag="ACHOQY_003033"
                     /gene_synonym="yebK"
     CDS             13460..14329
                     /gene="hexR"
                     /locus_tag="ACHOQY_003033"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9679089.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14457..15899
                     /gene="pyk"
                     /locus_tag="ACHOQY_003034"
     CDS             14457..15899
                     /gene="pyk"
                     /locus_tag="ACHOQY_003034"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9679090.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(15959..16801)
                     /locus_tag="ACHOQY_003035"
     CDS             complement(15959..16801)
                     /locus_tag="ACHOQY_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP9679091.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(16824..17663)
                     /locus_tag="ACHOQY_003036"
     CDS             complement(16824..17663)
                     /locus_tag="ACHOQY_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9679092.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT         4479 a         4498 c         4242 g         4479 t
ORIGIN      
        1 cttagttgcc ttgcccttta agaatagatg acgacgccag gttttccagt ttgcgtgcaa
       61 aatggtcaat aaaaagcgtg gtggtcatca gctgaaatgt taaaaaccgc ccgttctggt
      121 gaaagaactg aggcggtttt tttattggaa atcaaaaggc tattttaggt aattaacaga
      181 gtttttcagc tcgttctata aacggtgcca gactcatttt ttcgccggga ttgttaggat
      241 catcaatctg aatcaccgaa atgggttggg ctttagtctt cccactggca acttcctttt
      301 gtgcgatatc gtttaaagga tactgcacga gggtacttgg gttaatgaca tacaaagcat
      361 tacccggtct gcaagtcagc atcacctctt cgcgattaaa cgcccatttg tctttaccca
      421 cttcaaaacg actgacggta atcacctgcg gtgcagccag cgccgctgca gaactggtga
      481 gtaacagaaa cgtcagaata ctttttttca tcattttcgt aatccatcaa aaaggttcga
      541 gagtcgcaag caggctgatg atcaccagca ctaccgcacc tatcgcccat tcgagtttag
      601 tcatccactc aaaccagggt gccgcacgat cttcatcttg cctcatgcgc ggtacaagaa
      661 cataacgatt agccagcgca atgaccacca taatcatcac caggatggct ttgagcaata
      721 agagttggcc ccagtaagtc gtaagcgtgg ggggaaatcc ggtaatcaac aatgcattaa
      781 gcacgccgct tgccagtacg ccgatcaccg caaaatgccc gcaccaggaa aaacgcatca
      841 gcgcctgaat ggcctggtgt cgccaacggc ctttgatgag ttgcatacac cagagcaccg
      901 gcagtaatcc cccaaaccac gccgccgcac aaatcaggtg aatcgcgtga ttagtctgat
      961 ggattttcgc cgttacccct tcattcagtg tcgcatgccc gattcccgcc agcagaataa
     1021 attgcgcggt agtgagcata aacagcaaac gtggcatatt ccgcggttgc ataagggcga
     1081 cgatcaacgt tacgagggcg agaacaattt gccataacca gacgccacca aactgcgttt
     1141 gcaaaacagc ctgccaaatg tttggcgaaa atacatctgc ccatcccgtt cccatcaacc
     1201 cgccctgaac ggcgagcatt gcagtggcac tgatcaaact ccagacggca gcgtgctgtt
     1261 gtaaacgcag aaaacgcttc gccagcaagc gacgaatcgt taagggggcc agccaggcac
     1321 cgtacattgc gaagccaaag accagcatca gcgaggtgaa atggataaat cgcagcgcga
     1381 tccaggtaaa cgccagcatg atttatttca cgctaaaggt gtagtgtcct ttcgttttat
     1441 gcccgtccac cgaaacaaca tgccagtcaa cgatgtaggt tccgggtttc agcgaatcgg
     1501 ccagtggaac aatcagttgc ttttggtcct gctcatttcg cttcgccggt aatgttttaa
     1561 tattttcgtt ttttggcccc gtgatttttg caccactgaa tcctgtttca acaccttccg
     1621 agaagtttaa agtgattgcc tgcggtgcag ctgtcacttg cgcgtttgcc gcaggatact
     1681 gatgcgttaa atgcgcatgt gcccagacgg aaggtgtcac caacgaagtg gtcaggattg
     1741 ccagcgcgta gcgaagggag cgtgcagttg aagtcatatt atctattcct ttttgtaata
     1801 acttttttac agagcataac cttgtctaat gtctgagtcg aggatcatca attccggctt
     1861 gccatcctgg ctcactctta gtaacttttg cccgcgaatg atgaggagat taagaatgct
     1921 gaagaatctg gctaaactgg atcaaacaga aatggataaa gtgaatgtcg atttggcggc
     1981 ggccggggtg gcatttaaag aacgctacaa tatgccggtg atcgctgaag cggttgaacg
     2041 tgaacagcct gaacatttgc gcagctggtt tcgcgagcgg cttattgccc accgtttggc
     2101 ttcggtcaat ctgtcacgtt taccttacga gcccaaactt aaataaaact tatacagagt
     2161 tacactttct tacataacgc ctgctaaatt atgagtattt tctaaaccgc acccataatt
     2221 tgcagtcatt ttgaaaagga agtcattatg tcgttctgga aagttgcagc agcgcaatat
     2281 gagcctcgta aaacctcact caccgaacag gtagctcatc atctggagtt tgtgagggcc
     2341 gccgccaggc aacaatgtca gctgttagtt tttccatcgc tttctttact ggggtgtgat
     2401 tattcgcgac gtgcccttcc tgccccaccc gatctctcac tgttagaccc gctttgttat
     2461 gccgcaacga cctggcgaat gaccatcatt gccggccttc ccgttgaata taacgatcgc
     2521 tttattcgtg gaattgcggt gttcgcaccg tggcgaaaaa cgcctgggat ctaccatcag
     2581 agtcatggtg catgtctggg tcgtcgttcc agaaccatca cggtggtcga tgaacaaccg
     2641 caaggtatgg atatggaccc gacctgttca ctgtttacta cagggcaatg tctgggagaa
     2701 cccgacctgc tggcgtcggc ccgtcgctta caattttttt cacatcagta ctcgattgcc
     2761 gtgctgatgg caaatgcccg tggtaacagt gcactatggg atgaatatgg tcgtctcatc
     2821 gttcgcgccg atcgtggttc attattgtta gtcggtcagc gttcatcaca gggttggcaa
     2881 ggcgatatca ttccattacg ctaggctttt tcggcctgga gcatgccatg ttgcgcatta
     2941 tcgatacaga aacctgcggt ttgcagggag ggatcgttga gattgcctct gttgatgtca
     3001 ttgacggaaa aatcgtcaac cccatgagcc acctggtgcg ccccgatcgt cctattagtc
     3061 cacaagcgat ggcgattcat cgcatcaccg aagccatggt cgccgataaa ccgtggattg
     3121 aagatgtgat cccacactat tacggtagtg aatggtatgt cgcgcataac gccagctttg
     3181 accgccgcgt actgcctgag atgcccggtg agtggatttg cactatgaaa ctggcccgtc
     3241 gtttgtggcc tgggatcaag tacagcaata tggcgttata taaaacacgc aagctcaatg
     3301 tacagacgcc gccgggcctg catcatcacc gcgcgttgta tgactgttat atcaccgccg
     3361 cgttgcttat cgatattatg aacacctccg gctggacggc agaacagatg gccgatatca
     3421 ccggacgtcc gtcgttgatg acgaccttca cctttggcaa ataccgtggc aaagcggttt
     3481 ccgacgttgc cgaacgcgat ccgggctatc tgcgctggtt atttaataac ctggacagca
     3541 tgagcccgga gctgcgttta acactgaaac attatctgga aaatacttag tccgcaggcg
     3601 tagcgggtaa tgttccctgc gccagcgcga ccagaaaagc atattccatc gctacgcctt
     3661 cgtacgattt aaagcgacca gatttaccgc catggcctga gtccatgtcg gtacagagca
     3721 ataaaagatg gtcatcggtt ttcagctcgc gcaatttagc gacccatttt gccggttccc
     3781 aatattgcac ctgagaatcg tgcaaaccgg tcgttaccag taaatgcgga taagcctgtg
     3841 cggtgacgtt gtcatacggg ctgtagcttt tcatgtactc gtaatattgc ggatcctgcg
     3901 gattacccca ctcttcaaac tcaccagtgg taagaggaat tgattcatca agcatcgttg
     3961 ttacaacatc aacaaacggt acctgggcga taacgccgtg gaataattcc gggcgttgat
     4021 taattgcaac gcccatcaac atgccccccg cactcccgcc catcgcataa caaagcgaag
     4081 gagagccata gcccagtttt aacaatgcat cgcaggcatc aagataatca ttaaacgtat
     4141 ttttcttctt cagaaatttt ccgtcttcgt accattgttg ccccagctca ccaccgccgc
     4201 gaacatggac aatggcgtag acaaagccac gatctaacaa actcaagcgg ctaaaactga
     4261 aatcggcatc aatacttgcg ccgtaagaac catagccata caccagcaac gggttgtgtc
     4321 ctttgcgaaa atgtttgcga tggtagacca acgaaaccgg aacttcgacg ccatcacggg
     4381 cgactatcca caggtgttca ctgcggtaat tcgccgcatc aaaaccgggc acttctgttt
     4441 gttttaatac acgacgctca ccggtatcca tatccagttc aaacaaagtg tctggtgtgg
     4501 tcatggaaga ataaccataa cgcaatcgcg cggtttcagg ttctggattg taggcaatcc
     4561 aggtcacata ggctggatca tcaaaggcaa taccaatgac ttcccgggtc ttgcggttaa
     4621 tttggcgcaa actggttaac ccgcgctgac gctcttcaac caccagccag tcggtaaaca
     4681 gcgtaaaccc ttccagcatg atgttttcgc gtggcggaat taactcttcc cactgttgct
     4741 catcacgcat acgggtacgg tataagccaa agtttttgcc gtggcggttg gaacgtagat
     4801 aaaaacgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc ggcagaaaaa
     4861 caaacggctc ggcatcggcc atttccgcgt ccagcaggcg aacttcactg gtggtggcgc
     4921 tggccaaatg aatgactaca tagtgcttcg aggtcgtttt atgcaggctg acgtaatagg
     4981 tatcgtcttt ttcttcgtag atcagtttat cttgcgatgc tggcgtaccg atggcgtgac
     5041 gccagacctg ataaggcagc agcgtcaccg gatgcttgcg aacatagtag aaagtccagg
     5101 agtcatttgc ccagacaaag ctgggttcaa cgttatccag cagttccggg taccagttac
     5161 cagtttccag attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa tcttctgcca
     5221 gcgccataat ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa aactcactat
     5281 gagctgcgcg cttattggca tcgagcaatg tttcccactc atcccactct tcactgaatg
     5341 ccgattgacg ctggtagata gcatattcac agcctggttc ataaatatgc cgatagcggt
     5401 agccattttt gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg atgatttcct
     5461 ttaagatgcg atcctgcaag gcttgttgtg aggccatcac ccgatgaccg taactatttt
     5521 cttgttgcag gtagtccagg acttctggct gagaacgcgt atcgtcccgc agccagtagt
     5581 aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg gcggcttttg
     5641 gtagcatgtt attgttcttt ctggttgaaa catcttataa gggtggcaaa actcaccggg
     5701 gatgcaagcg aaacagggga gtcattgctt agatgatgac aggtaatggc gcggatatcg
     5761 aatgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgcgattc tggatagccc
     5821 gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt gcgtttttgc
     5881 tgttcgagat cgcgttcaat gccgtcacga acatcctggg ggattttcag cgcgtcaccc
     5941 agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc gcaactcagg
     6001 aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaggcg ttgtggatcc
     6061 agcggttgtt cgattgcctg ctcaatgagt acacgcccct gctcttccac gccggcttca
     6121 cgcaattgct ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc acttttagcg
     6181 gcaaagacca gcgcaaggat caaacgttct gtacgctcat ccagcggcgt actttgcgcg
     6241 ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt attccacagc
     6301 accgtacccg ctaccgctcc gccgccaacc agtaacgcgt tggtgccata tttcgtaaga
     6361 agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa tgcgcctggc
     6421 actaacagtt tgcccaatcc ctgatccgca gacgaggagg tagaagaact gctttgccca
     6481 agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata accattttta
     6541 gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa aagatgcgca
     6601 aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg tatttactgt
     6661 atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt ggcattgttt
     6721 gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt
     6781 ctggggcgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt atcagaagcc
     6841 attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc tggtaatgaa
     6901 tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct ttaccctgaa
     6961 tgtcctgcaa actgacccaa gccacgggat cggcctgacc gacaattttt tgatcatctg
     7021 cccaacgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca attccggcag
     7081 catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta ttggcagcga
     7141 aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt tttttcatgt
     7201 ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag gagcgcaagt
     7261 tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg acctgtgggg
     7321 acggtaattt gccggttttc agaatggtgc tcagcacatc tttttgttct gccagccata
     7381 gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc agccaggtgt
     7441 caagcagatc cgccgtatca agcattttat cataagcatc ggcttccttt ttgctggtaa
     7501 acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc gccatttgca
     7561 gcctctcata ataactgtga ttttatacag tatatttctt ttcggttgag aaatcaacat
     7621 cagcaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt aatcagggga
     7681 tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat
     7741 taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg
     7801 tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat cgctcccatg
     7861 tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa aaaccacatt
     7921 atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt gaagaggaag
     7981 gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc
     8041 gtcgcctggc ggcagaacag ctgcagctgc ccacttccac ttatcgtttt gccgatagcg
     8101 aaagcctttt ccgcgaggcg gttgccgcca ttggctatcc ctgcattgta aaaccggtga
     8161 tgagctcttc cggcaagggg cagacgttta ttcgctctgc agagcaactt gctcaggcat
     8221 gggaatacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg
     8281 ttaagtttga cttcgaaatt accctgctaa ccgtcagcgc ggtggatggc gtccatttct
     8341 gtgcaccagt aggtcatcgc caggaagatg gcgactaccg tgaatcctgg caaccacagc
     8401 aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac
     8461 tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca
     8521 gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct caagatctct
     8581 cagagtttgc cctgcatgta cgtgccttcc tcggacttcc ggttggcggg atccgccagt
     8641 atggtcctgc agcttctgcc gttatcctgc cacaactgac cagtcagaat gtcacgtttg
     8701 ataatgtgca gaatgccgta ggcgcaggtt tgcagattcg tttatttggt aagccggcaa
     8761 ttaatggcag ccgtcgtctg ggggtggcac tggctactgc agagagtgtt gttgacgcca
     8821 ttaaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc cggcaaaaaa
     8881 acgctacaaa aatgcccgat ccgcgatcgg gcattttgac ttttacagct tagcgccttc
     8941 tacagcttca cgcgccagct tagtaatacg gtcgtaatcg cccgcttcca gcgcatctgc
     9001 cggaaccagc caggaaccac cgatgcacag cacgcttttc agcgccaggt agtcacggta
     9061 gttagccgga gaaataccac ctgtcgggca gaaacggacc tgggagaacg gacccgcgat
     9121 cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga actctttcaa
     9181 accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa tcagaggaat
     9241 agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg
     9301 tgcacccgct tcggtgactt ctgccagctg ctgtggattc agcaccgtac cggcacccac
     9361 aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac actcggtacg
     9421 cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca tcggcactgc
     9481 gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca ggattgattc
     9541 tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa atttgtcgtc
     9601 ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac gcaaggcgct
     9661 gaataattca cgtcctgtcc ccacgcgtga cgcgctcagg tcaggaatgt gcggttcgcg
     9721 agcagccagt tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc cattcacacg
     9781 aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt ctggtgttac
     9841 gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg taactaacgc
     9901 aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat gtaattctgg
     9961 cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc ggtccagcaa
    10021 acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg ctggcgcttc
    10081 aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc ccaggttacc
    10141 gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag cgatcacatt
    10201 gctgtcgagt gatttttccg ccccttcccg ccagtccagt tcaccattat tcagccatgg
    10261 ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt catgcagcag
    10321 gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct ggaagtggtt
    10381 aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat cagaaaggtc
    10441 agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat
    10501 ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca ccaccacttt
    10561 ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca tgcgtgtaac
    10621 ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga
    10681 agagcctggc aactgcatcc ccataaattc caccaccatc tggttggtgt tggcagtacc
    10741 gtagaaagta catgttcccg gcgcatggta agacgcggct tctgactcca gtaaggccat
    10801 gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt tatttggcaa
    10861 accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa acgacagggc
    10921 tgccatcgtc agacccggga caatcttgtc gcacacaccg aggaacagag caccatcaaa
    10981 catgttatgg gacagcccga ccgccgcaga catcgctatc acttcgcggc ttagcagcga
    11041 caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa caccgcccgc
    11101 aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg gatagtgttc
    11161 ataaggctgg tgcgcggaga gcatgtcgtt ataggaggtg atgatggcga tattgttacg
    11221 caacatgctt ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag
    11281 gttaccgcat gccaactgcg aacgatgaac ggtcgaagtt ttcgcttgtt ctatccgggc
    11341 gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta cgcgtaacaa
    11401 ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt cacaacgcgt
    11461 ttcattcaga ggatttatga ctgaaacgcc tgtaaccgga gctcataggg caaaaacgtt
    11521 tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa
    11581 aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa aggataagcg
    11641 cagatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg ccaccgaggc
    11701 aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca tcgcccacgc
    11761 ctcagtaatg gagtctaccc atttccaggc ttcttccact tcgtcgcgac gaacaaacag
    11821 tgcctgaata ccacgcatgg tttccagcag caaacgttca taggcatccg ccagatgcgt
    11881 ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt tatgtttgtg
    11941 gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag gttgcagacg
    12001 gatagtcagt ttattctgcg gcagatcctg ccacgattct ttaaacagat tcagttcagg
    12061 tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac cagtacgcag
    12121 gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg ctacgaaggt
    12181 ttctgtattg ctgctcttgt tcgcgccctc ttcttccaga tatcccggca cttttttgcc
    12241 ctgggcgaag cccgcagtat attgcccgcg tacggttttt tcgcgtacgt tggagcggtc
    12301 gatgcggcgc agagacttca gtactttcac tttttcatcg cggatgctgt ctgcgctcag
    12361 gtcagacggc ggagacatcg caatcatgca gagaatttgc agcaggtggt tttggatcat
    12421 atcgcgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc ccacttcttc
    12481 tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca caaacaggga
    12541 gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat agtggtcgat
    12601 acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt cctgcgaggt
    12661 cgccagcgac gtccccagcg gtttctccat gactacgcgt gccggtttag cattcagttt
    12721 agcctcgcca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt
    12781 aatggtgata cgattttttt gatccagcat cgcgccgaga cggctgaatg cagcagtgtc
    12841 attgacatcg agattacaaa aatccagacg tgcactcagg gtgtcccata aaccttcatc
    12901 aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca actttggtat atgccgcttt
    12961 atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct gaccggcttt
    13021 ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa
    13081 aatgaccagg tcacaggcct gggctgtttg cgttaccgcc atgtcattct ccttaagtta
    13141 actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca ctgtactgct
    13201 tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg taaaagcgcg
    13261 caaagtgcgg caaaactgat aaaaaaatca tcgttttcca ttgggtaaaa atctgacact
    13321 gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta acacagcaca
    13381 caggcgtaat atctgacaaa actacatttc gatttctttc agtgcggaaa tcgtcattac
    13441 ccgtgagttt ctttacatca tgaatatgct ggaaaaaatc cagtctcagc tggaacattt
    13501 gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata acgcgatcca
    13561 ttcgagtatt gctgctctgg cgctggaagc caatgttagc gaaccgacgg tgaatcgttt
    13621 ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg cacaaagtct
    13681 ggcgaatggc actccctatg ttaatcgcaa tgtcaatgaa gatgacagcg ttgaatcata
    13741 cacagggaaa atatttgagt ccgcaatggc aacgcttgat catgtccgtc attcactgga
    13801 taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt
    13861 cggattaggc tcttcagccg ccgttgccca cgatgcgatg aataagttct ttcgttttaa
    13921 tgttccggtg gtgtactccg atgatatcgt gctgcaacgc atgagttgta tgaattgtag
    13981 cgacggagac gtggtggtgc tgatttctca cactggaaga acaaaaaatc tggtcgagct
    14041 ggcgcagctg gctcgcgaaa acgacgccat ggtgattgcc ctcacctctg cgggtacccc
    14101 gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata ctgacattta
    14161 tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg cgacaggatt
    14221 tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg aagcgctgaa
    14281 ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa aacgactgtc
    14341 actgtcctaa tcttatacga catccgaatg agattaattt atcgccatcg cggcgttatt
    14401 tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag tattacatgt
    14461 ccagaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca gatcgcgata
    14521 ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg
    14581 gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg
    14641 ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta tccaccttta
    14701 aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc aacctgggta
    14761 aaggtgaagg cgacaaagaa aaagtcggta tcgactacaa aggcctgcct gctgacgttg
    14821 tgcctggcga catcctgctg ctggacgatg gtcgcgttca gttaaaagta ctggaagttc
    14881 agggcatgaa agtgttcacc gaagtgaccg tcggtggtcc cctctccaac aataaaggta
    14941 tcaacaaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca
    15001 ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca cgctgcggcg
    15061 aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg aaaattgttg
    15121 ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac atcatcctcg
    15181 cctctgacgt ggtaatggtt gcacgcggcg acctcggtgt ggaaattggc gacccggaac
    15241 tggtcggcat tcagaaagcg ttgatccgtc gtgcgcgtca gcttaaccgc gcggtaatca
    15301 cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt gcagaagtca
    15361 tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct gcagaaactg
    15421 ccgctgggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg
    15481 aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc gacaatgtgg
    15541 aagaagctat tgccatgtca gcaatgtacg cagctaacca cctgaaaggc gttacggcga
    15601 ttattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc
    15661 taccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct ctctatcgtg
    15721 gcgttacgcc ggtgcacttt gatagcgcta atgacggcgt agcagctgcc agcgaagcgg
    15781 ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt gtcacccagg
    15841 gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tattttaacg gtagagtaat
    15901 ctcacctgcc ccggccgctg atgaccgggg tatttagcaa cacataaatt aggactactc
    15961 attcaatcca atcataatca cagccccctg taaaatgaat acagggatag cctgaatgga
    16021 gatctaaata gcttgcaaga cgtcgcatac ccgcttcctc tgcaacacta tatccaataa
    16081 ctgccatcgg taagccacta tctttggagt attgtgtctg cacccatgtt ttaattgaat
    16141 cgttaatcgt aatataataa tcagcctgat attccatata ttgtattgga tcacaaaaac
    16201 agcctgcccc aatcccaata gttctaacta ttctggctct atctccataa aattggatcc
    16261 caggaagatt ataaattttc aaatttttgg caaaacgttt ggtaatatta atggcactgt
    16321 caggttctat agcatataca tggtacatat catcagaagc aacaatattt gtaacatcca
    16381 gttctaactg ctgagccaaa gccttagaca ttccgaacgt aggctctcga tccatgacat
    16441 catggcaacg aatgattacc aaattatttt tatgcaaaaa ctcctttttt tgctcaatta
    16501 tttttaaata actgagttca ccgcgcgtat aatccattat ttttctggcc catgaaaact
    16561 caagaacgtc accctccaga tcagcataag aataaaatgt aggctcatga gtaataatga
    16621 tattggcacc ttcaaaaaca gcctttctta aaacatcaat tgaggcaatc cagcaggtcc
    16681 caattttatt aataagtgtc gcctcagcac caaaaataat tttatcaaca gttggttgcg
    16741 aaacggcaac cattgaattt aaaaaacaat taagatctgc ggctgtttta atatgatgca
    16801 ttcaacatcc ttcaccggtt tcatcagcaa taaaataatt tcaacatttc tggaaaacgt
    16861 tttgcccatg cagactcaga gtgggtatca cctccccata gatgataatc tatagttaca
    16921 ttgcgttgtt tacggagact cacgcataat ttttccgcgc ctgaaatata gacttcagga
    16981 aagtcttcgc gcataccact ggtttccagc gtaccgacat cgagatatac agtaagatct
    17041 gcaaagatat tgttttcctg aataaaattc agcattgcgc tgtcattgaa ccatagtgcc
    17101 ggcgaaaaaa cgccaacttt tgaaaaaacg tctagacgaa gaattgcggc aaacaaactg
    17161 atgtacgcac ccatagaact acctgctatc gctgtattat tacgatctgg gagagttcga
    17221 aaattgctgt caatatagtt cttgagcgta ttcacaataa aatcaacata caacgcacct
    17281 tttcctccac tataatccac atcagggtcc cagccaggta agggaaaaga tgtgtttagc
    17341 ggccatggag aatactcatc catacggctg aatttaccat gagacgatgc attatcaatc
    17401 cccacaatga taacaccgtc gtattgggaa tttgggaaaa aagcatctac cgcctcaggt
    17461 atatcccata catgtggacc aaaagataca gatttatcaa ataaattttg cccatcgtgc
    17521 atatacaata cagggtaact gacatcagat tcataatagg acgcaggtag taatattctt
    17581 atttcgcgac tacttgccaa ttgtggcatc ggaaaggatt gcaacacatg atatcgattc
    17641 ccatagttct caatatcgtt cataatttaa attaacctca ataaacaaaa gaaatatt
//
LOCUS       DAZKGS010000091        17663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKGS010000091 DAZKGS010000000
VERSION     DAZKGS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..753
                     /locus_tag="ACHOQY_003037"
     CDS             37..753
                     /locus_tag="ACHOQY_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9679093.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            783..1283
                     /locus_tag="ACHOQY_003038"
     CDS             783..1283
                     /locus_tag="ACHOQY_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFP9679094.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1375..4059
                     /locus_tag="ACHOQY_003039"
     CDS             1375..4059
                     /locus_tag="ACHOQY_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFP9679095.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4056..5130
                     /locus_tag="ACHOQY_003040"
                     /pseudo
     CDS             4056..5130
                     /locus_tag="ACHOQY_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5218..6516)
                     /gene="exuT"
                     /locus_tag="ACHOQY_003041"
     CDS             complement(5218..6516)
                     /gene="exuT"
                     /locus_tag="ACHOQY_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9679096.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6999..8411
                     /gene="uxaC"
                     /locus_tag="ACHOQY_003042"
     CDS             6999..8411
                     /gene="uxaC"
                     /locus_tag="ACHOQY_003042"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9679097.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8426..9913
                     /gene="uxaA"
                     /locus_tag="ACHOQY_003043"
     CDS             8426..9913
                     /gene="uxaA"
                     /locus_tag="ACHOQY_003043"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9679098.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9996..10547
                     /gene="ygjV"
                     /locus_tag="ACHOQY_003044"
     CDS             9996..10547
                     /gene="ygjV"
                     /locus_tag="ACHOQY_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9679099.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10552..11796)
                     /gene="sstT"
                     /locus_tag="ACHOQY_003045"
     CDS             complement(10552..11796)
                     /gene="sstT"
                     /locus_tag="ACHOQY_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9679100.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12195..13160)
                     /gene="alx"
                     /locus_tag="ACHOQY_003046"
     CDS             complement(12195..13160)
                     /gene="alx"
                     /locus_tag="ACHOQY_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9679101.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13443..14429)
                     /gene="ygjR"
                     /locus_tag="ACHOQY_003047"
     CDS             complement(13443..14429)
                     /gene="ygjR"
                     /locus_tag="ACHOQY_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9679102.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14508..15187)
                     /gene="ygjQ"
                     /locus_tag="ACHOQY_003048"
                     /pseudo
     CDS             complement(14508..15187)
                     /gene="ygjQ"
                     /locus_tag="ACHOQY_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15264..15767)
                     /gene="ygjP"
                     /locus_tag="ACHOQY_003049"
     CDS             complement(15264..15767)
                     /gene="ygjP"
                     /locus_tag="ACHOQY_003049"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9679103.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15852..16988
                     /gene="rlmG"
                     /locus_tag="ACHOQY_003050"
     CDS             15852..16988
                     /gene="rlmG"
                     /locus_tag="ACHOQY_003050"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9679104.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17179..17262
                     /locus_tag="ACHOQY_003051"
                     /pseudo
     CDS             17179..17262
                     /locus_tag="ACHOQY_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17259..>17663
                     /gene="higA"
                     /locus_tag="ACHOQY_003052"
     CDS             17259..>17663
                     /gene="higA"
                     /locus_tag="ACHOQY_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9679105.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPAL"
BASE COUNT         4687 a         4358 c         4490 g         4128 t
ORIGIN      
        1 aactttttgg aaacaatatt gtaagatcaa atttatatgg tggcgaaaat taatctctta
       61 tcacggctta cgccgctttt attcgtgttc gctccttttt tggcacagtc aaatatgact
      121 gtttatccta tggctgtttc cattaatagt cagggtgaag gcaacgttcg cgtaatatct
      181 aaaagtaatg aagttcagta catcaaggcg acagtattcc gtatcgataa tccatcgacg
      241 cctcaggaaa atgaagttga gattaaatcc ggtgatgcaa atcatctggt tgttatgcca
      301 cctaaattcg ctttaccagc cggtagtagc aaaaccgtac gttttgttgc gatggaacca
      361 gagcaaaaag agaaaaatta tcgcgttaaa tttgaagcgg ttcccagtat tgatgacgtt
      421 gccacagata aaaaagatct ctctatgcag ttaacagtta acttaatttg ggggattgtt
      481 gttagtgttc cacctcagca acctattgct aagttagaag taaatgctgc ccaaaaatta
      541 gttaatgcag ggaatcaacg cttaaagatt ttaacgatcg cttattgtaa aaataatagc
      601 aaagaaaatt gtaagataca gaccgtaaat aaaaacatct tccctggtca ggaaagaaat
      661 cttgaaagca tatctggcta cgacaaaatc gttgtcaaat ataataactg gatcaccaaa
      721 gataatggcg agtttgaact ggcggtccat taattatcat tgtaataaac gaaggaatag
      781 taatgaaaaa ggtatttgca aaatctcttc tggtcgcagc gatgttttct gttgcaggct
      841 ccgcattggc agtacaaaag gatattactg taacggccaa cgttgatgcc gctctggata
      901 tgacacagac cgataacacc gcgttgccga aagcagtaga aatgcaatat ctgccggggc
      961 agggtctgca atcttaccag ttgatgacca aaatctggtc taacgacgtt accaaagatg
     1021 taaaaatgca gttagtttcc ccggcgcaac tggttcaaag cctggatgct agcaagattg
     1081 ttccgctgac agtaacctgg ggcggtgaag aaattaaagc tgatgcggca accactttta
     1141 ccgcaaccaa aatctttgct tccgatgcgt taactaacgg ttctctggct aaaaacctga
     1201 tgtttgctca aatcaccaaa ggtgttctgg agacaggcat ctaccgtggt gtagtaagca
     1261 tttatctgtc tcaggatatc taatccagat aacagaatta acacgtaaca aagggagggg
     1321 tagccctccc ttattttaac tgttttagta tttgttatgg atttcaggat tgccatggat
     1381 aaaaaattac tggctctttt gatcctggcg agtctcagcc cggcagaggc gacattaacc
     1441 aaaattcccg cagggtttga ggttattgct cagggacagc aggagtatat cgaggtttat
     1501 ttttcaggga aaagtctcgg taaatattat gcaatggtta atcttgatac cgtaacattt
     1561 cttgatccag caagtctata taacaagctg gaactggatg tagacgatca gaaaatcgcg
     1621 catatagtga aagaaaaatt atcgcagccg ctagctcgcc acggtgaatt ggcttgcggt
     1681 tatgtacgta ctgactcagg gtgtggtttt ctgaataccg atacgctgga aataatctat
     1741 aatgatgaag aaagttcggc aacgttgttt attaatccgc aatggaattc agctttcgat
     1801 gcgaagtcat tatatttaaa tccagacaaa aatactgtta atgcttttat acatcagcaa
     1861 gacatcaatg ttctggcaca ggatgattac caatcgttgt ctattcaggg aaacggtgcg
     1921 ctgggaataa cagaaaatag ttatattggt gcacactgga atttcaacgg ttatgatgca
     1981 gatgatgtca gtgacagtaa tgctgacatc agcgatctct attatcgtta tgatttttta
     2041 cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca cactatttaa tgcacaaggc
     2101 gggaacttta cctttaactt tctgccactc ggtgcaattg atgggatgcg tatcgggtcg
     2161 acactgagct atttaaacca ggcgcaaagc cagcaaggaa ccccagtaat ggttctgctt
     2221 tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac ttttgggatc gttttatctc
     2281 aatagtggtt cgcaatttat tgataccagt tcctttccgc caggcagcta cagcgtggca
     2341 ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc ttgtgccgtt taccaaaacc
     2401 ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg caggtaaaac tacatcacag
     2461 gtttctgatg atgaaagttc agcttatcag ctaggagtac gcctgccatt acatccgcaa
     2521 tatgagctct acgcagggct ggcgaatgcc gatgatgtga gtgctttcga gttaggtaat
     2581 aactggacgg cagatttagg cggggtgggg aatcttgcaa tcagcgccag cgtgttccgt
     2641 aacgatgacg gcggcaaagg tgatatgcaa caggccaact ggagtaatcc gggatggccg
     2701 acgttgggct tttatcggac caactctgac ggcgatgctt gtacaaccga cagcagagag
     2761 agctataacg ccttaagctg ttatgaaagt atttccgcga cggtttcaca gaattttgtc
     2821 ggctggaata tgatgctggg ttatacccgc acacaaaata acactgatga tagtttgcgt
     2881 tgggataaac agcagagctt tgaaaataac tatcttcgcc agacaacagc gcaaagtatc
     2941 tctgaaactg tacaacttag cgcttcccgc gcttttgtga tgcgtgactg gattttgagt
     3001 acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg ataacgacga caacggcttg
     3061 tacttatcgt tttcgttatc tgacacgcca acgatggata gcaataacaa cagccattca
     3121 acaaatgtgt ctacggatta tcgttatagc gaacaggatg gcgatcaaac gtcatggcag
     3181 ttatcgcata ccttttataa cgattcattc agccataaag aacttggcgt aaccgtcgga
     3241 ggcctgaaca ccgataccat aaacagcgcg gttaacgggc gttgggatgg gcaatacgga
     3301 aatgtctacg ctaccgtatc tgatagttat gaccgtaaga atcatgatca tctttcggcc
     3361 tttacgggca cttatagctc tacgctggct gtcagtcgct atggcgttaa tttgggagcc
     3421 agtggtacag acggtttgct gggtgcggta ctggtggatg tgaaaggctt ctctgaacag
     3481 gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg caggcagccg aactttgcag
     3541 cttggtcaaa gtgacagtgt gttgttccct tatcctggat ttcagtctgg ttttgttgag
     3601 gttaacgaca gtagccaggg caatcagcag gggacaacaa acatcattaa cggtgcaggg
     3661 aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc gcgaagtgtc tgccagcttt
     3721 aactacaact atatcggtcg tttattactg ccggcagcgg tgaaaaaatt cccgattgtg
     3781 gggctgaaca gcgccatgtt actggtagct gaagatggcg gatttacact tgaaattaac
     3841 ggcagcgaaa aagagctcta tctgctttcc gggcagcaat tccttaagtg tccactgagt
     3901 gttgtaaaga aacgcgccag cattcgttac agcggagatg ttacttgtag tgtggtgact
     3961 tattcacaat taccggagtc gattcaggtt caggcacagt tgaaacagcc taaattacgt
     4021 ggaaacgttc agacggcgca aagggaggtt gcaccatgag aaatcgattg attgcggcga
     4081 tattgggctt gtttggttca ggcagctcct gacgtgacca gtgaaattac gtatgatttg
     4141 gcatctggta gagcggatta ttacttctgg aaggatgaag cgtcggcagg aaataatgga
     4201 tatatgtggt atgaatgttc gtatcctgac ctccaacaaa cctgtacagc taatggaaat
     4261 atatcgacag tacaaatcta tttaactgaa caacgcagtg ggatgcgttg gccggtaaaa
     4321 ctcaaaggat ttaaaacagc cattgtaagt agcgatgaag cgccgccagg atgcaagggg
     4381 ggcaaagggc ttcagacgaa tcttaaggat tctaatagat cttcatgtac agaagatggt
     4441 caacattatt atatatacga tacaaagttt cttacgctct accttgagca gacagagatg
     4501 aagaatttgc cgattggtgg cgtctggaag gggaaagtta aattacattc gaacagcccg
     4561 gcccaggact atttcgcaaa tattaccctg aatacgctcg accccaacca tattgacgtg
     4621 ttcttcccgg agttcgccca cgccacgcca agggtgcagt tagacttgca tccaacagga
     4681 agcgttaacg gcagcaacta cgcgcaagat ctgaccatgt tggacatgtg cctgtacgat
     4741 ggttttaacg gtaatgccat cagttatgaa atcatgctca aagatgaagg gcgaccagct
     4801 gcagggcgca gagacggtta cttctctatc tatcgtcagg gagggaccac caccgacgag
     4861 ggagaacgca ttgattaccg ggtcaaaatg tacaacccgg aaaccggtgg gcaaattgat
     4921 gtgcgcaata atgaaaatat ggtctggaac agcattaacc tgaaacgtgt gcgtccggtg
     4981 gtactgcccg gtattcgcta tgccgtgatg tgtgtgccga cacccttgac tctggcagtc
     5041 gataaattca gcgtgatgga taaacaggcc gggtactaca tgggcaaatt gtcagtaatc
     5101 tttacgcctt ccttgccaac catcaattaa ccctgcaaac cggacaaacg cttttttcta
     5161 tataaacgac gaagccgtct gataaagcgg cttcgtcatt acaacttaag ttaaacgtta
     5221 atgttgcggt gcgggatcgt tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg
     5281 caacacggtc cagataacca gcgcacctaa caggtcgaac actgccagaa ctgcgaacag
     5341 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
     5401 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
     5461 gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca
     5521 cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat
     5581 cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
     5641 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
     5701 cggcatccag gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa
     5761 cagcgggatc cacgcgttaa aagtacccca ggccggttct gccaggaaac gcggcagcgc
     5821 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct
     5881 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     5941 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     6001 tgcaccggag atgataaatg ccatctgcca gctgtgcatt acgattgccc ataccaccag
     6061 cggcggcgca atcatcgcac caatcgaaga acctacgtta aagtaaccta ccgcgatgga
     6121 acgctctttc gccgggaacc attcggagct ggctttcaga cccgccggga tcatcgctgc
     6181 ttccgcggca ccgactgcac cacgagcaat cgccaggcca ccccagctac cagccagcgc
     6241 ggttgcacca cagaacacgg cccacagcac agcaaacatt gcataaccga ttttcgtacc
     6301 cagcacatcc agtacataac ctgctaccgg ttgcatgacc gtataagcag cagaataggc
     6361 tgcgatgata taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc
     6421 agctgccgcc acagtgttac gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc
     6481 gatcatatac caacgtaacc ctttaatttt acgcatgtaa aacctcatcg ttttgttata
     6541 tccgcaggaa gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta
     6601 accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt
     6661 ccgtaccggt taacccgatt catctaacaa atgaatgaat cggcaatttg cattccgtcg
     6721 acttatgatt tgcgacggca gaaagataac ttgtcataca actttaaaag gtgagagcca
     6781 tcacaaatgt gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg
     6841 cggcctgcag agagatttac cactttgaga gtaatttttt taactacgtt tattgatcta
     6901 actcacgaaa atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc
     6961 ataagcaagc tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag
     7021 atttcctgtt agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc
     7081 cgattttcga ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta
     7141 aaaacctgta tgacatctgg ctgaaaggcg atcactacaa atggcgcgct atgcgtacca
     7201 acggtgtggc cgagcgtctg tgtaccggtg atgcgtctga ccgtgaaaaa tttgacgcct
     7261 gggcggcgac tgttccgcac actatcggca acccgttata ccactggacg cacctcgaac
     7321 tgcgtcgtcc gtttggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct
     7381 ggaacgaatg taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc
     7441 agatgaacgt gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg
     7501 cagagatcgc caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca
     7561 aagccttcaa catcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt
     7621 ccgataccga cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc
     7681 acttcgccgc tcacggctgt aaagtgtctg accatgcgct ggacgtggtg atgtttgctg
     7741 aagcgaacga agcggaactg gacagcattc tggcgcgccg tctggctggc gaaaccctga
     7801 gcgagcacga agtggcacag ttcaaaactg cggtactggt gttccttggt gccgaatatg
     7861 cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc
     7921 agttcaaact gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcag
     7981 aagagttgtc taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc
     8041 tgtactgcct gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg
     8101 gcgaaggtat gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag
     8161 acggtatgga acgtcagatg acccaactgg cgcagctcgg tttgctgagc cgctttgtcg
     8221 gtatgctgac tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca
     8281 ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc
     8341 tgctgggcga gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg
     8401 aactgaacta aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt
     8461 cgcggtcgct ttagcggatt tggctgaagg cacagaagtc agtgtcgata accagactgt
     8521 tacgctgcgc caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg
     8581 ggccaatgtc atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg
     8641 ggaacacgtc cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta
     8701 tcaacctgat tttcaggatc tgcctgcgca agcggcagat cgtgaagtgc agatctatcg
     8761 tcgcgctaac ggcgatgtcg gggtgcgtaa cgagctgtgg atcctgccaa ccgtgggctg
     8821 tgtcaacggc atcgcgcggc aaatccagaa ccgtttcctg aaagagacca acaacgccga
     8881 aggtaccgac ggcgtgttcc tcttcagcca cacctacggc tgctcacaac tgggcgacga
     8941 tcacatcaac acccgcacca tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt
     9001 gctggtgatt ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg
     9061 cgatatcgat cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga
     9121 agccgggatc gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc
     9181 aggcaaactc agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg
     9241 tattactgct aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac
     9301 taccgtactg accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg
     9361 ccgcgacgaa gcaacgtttg aaaaactggt gaccatggtc aacgacttca aacagtactt
     9421 tattgcccat gaccagccga tctatgaaaa cccatcgccg gggaacaaag cgggcggtat
     9481 caccacgctg gaagataaat cccttggctg tacccagaaa gcgggttcca gcgtcgtggt
     9541 tgacgtgctg cgttacggcg agcgtctgaa aacgccgggg ctgaacttgt taagtgcgcc
     9601 gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt
     9661 cagtactggt cgtggcacgc catacggcgg ctttgtaccg acggtgaaaa tcgctaccaa
     9721 cagtgaactg gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca
     9781 cggtaaagcg atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa
     9841 cggtaaacaa acctgcaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg
     9901 cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc
     9961 gccgtttgct tttccatacc tgataaggat ttatcatgac cgcgtattgg ctggcccagg
    10021 gcgtgggtgt catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc
    10081 gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg
    10141 gcacctatcc cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac
    10201 gcacgcgcag cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg
    10261 cgaagttcca tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg
    10321 cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct
    10381 gggtgattca caacttctgg gcggggtcga taggcggcac aatgattgag ggtagttttc
    10441 tgcttatgaa tggcttgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc
    10501 cgtttaaagt agagaaaacc cctcccgccg tagacgaaag gggttaaaca attaattacg
    10561 cagggcgcta tttgccagac gatcgtcttc cgcctggcaa gctgccgcag tgaacagcac
    10621 gtcagttgaa gagttcagcg cggtttcgca agagtcctgc aatacgccga tgataaagcc
    10681 gacggcaacc acctgcatgg cgatatcgtt cgaaataccg aacatattac aggccagcgg
    10741 gatcagcagc agagaacccc ccgccacgcc ggatgcgcca caggcacaca gagaagccac
    10801 cacgctcaac agcagcgccg tgggcagatc gaccgggata cccagcgtat taaccgcagc
    10861 cagcgtcaac acggtaatgg tgattgctgc gcccgccata ttgatggtgg cacccagcgg
    10921 aatagaaacg gaataggtat cgcgatccag attcagcttt tcacacagtg ccatattcac
    10981 cggaatgttc gccgcagagc tgcgggtgaa gaaggcatac acgccgcttt cgcgcaggca
    11041 cagcagcacc agcgggaacg ggttacgacg aattttccac cacaccagca atgggttaac
    11101 caccagcgcc accagtaaca tacagccaac cagcacgacc agcagttgcg cgtagcccca
    11161 cagtgtggag aaaccggtgg ttgccagggt agaagaaacc agaccaaaaa taccaatcgg
    11221 tgcgaagcga atgaccagtt tcaccataaa ggtgacggca ttcgacatat cgttgaccag
    11281 gtttttggtg gtctcgttac cgtgacgcag tgcgaaaccg aggccaattg cccacaccag
    11341 gatcccgatg tagttacctt tcagcagcgc gtcgatgggg ttggaaacca tgctcattac
    11401 cagcccgcgc atcacttcta caatgcctga cggtggcgaa atatcacccg cgctactgga
    11461 taagtgcagg gtagaaggga aggcgaagct gaaaacgact gcggccagag cagcagagaa
    11521 ggtacccagc agatagagga acaaaatagg gcggatattg gttttctgcc cgtgctggtg
    11581 gttagcaata gatgccatca ccagcatcaa caccaggatg ggggcaacgg ctttcagtgc
    11641 gccgacgaac aaagtaccta acagaccaac agcttccgcc gcgggttttg agatccatgc
    11701 cagaagaatc cccagaacaa ggccgaccag gatttgtttt accaggctgc catgagccag
    11761 acgccggaat agccccggtg aacgttgcgt agtcattttt cgatcctttc attgtgttgt
    11821 cgcacatccc tggtgcgttc tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag
    11881 gtcgagtata aggaaagtta cagcttcggg aagcgaaaaa tgctggattt taaagagagg
    11941 tcatattttg caacttaatg gttacattta tttgacataa aaataaagcc aacgtgcggc
    12001 ttccagactg atgacaacat caaaaaagga ctcggtcagt cccctcgccc cgtttgggga
    12061 gagggttagg gtgaggggaa caggccagca tcggtgcgaa aaattaccct caccccgacc
    12121 ctctccctgg aagggagagg gagaaaacgg tgctcatgtt aatgacaggg tacacggctt
    12181 aggcagatta aaaattatcc accccgctgc ttatcatgcc gataattcac ccaggcgttg
    12241 ataataaacg tcatcaccag aatgccaaac accacgccca gcgagacggc gattggaata
    12301 tggtagaagt cgacaatcag catcttgata ccgataaaca ccagaatcac cgccaggcca
    12361 tatttgagca tcgagaaacg ctctgctacg cccgccagca ggaaatacat cgcacgcagg
    12421 ccgaggattg caaacaggtt tgaggtcagc acaataaacg gatcagtggt cacggcgaag
    12481 atagccggaa tgctatccac ggcaaaaatc acgtcgctca attccaccag aatcagtacc
    12541 agcatcagcg gtgtggcgta cagcaatcca ttcttacgca caaagaaatg ctcgttgtcg
    12601 atggtgtcgg tcatgcgcaa atgaccgcgt agccagcgca ccagcggctt gtcgccaatg
    12661 cctgattcat cttcatgggc gagcgccatc ttcacgccgg taaacagcag gaaggcaccg
    12721 aagatataca ggatccagtc gaactgtgaa atcagccagc tgcctgtgaa gatcatgatg
    12781 gtacgcagaa caatcgcccc gagcacgcca tacaccagca cgcggcgttg taatgccgcc
    12841 ggaacagaga aatagctgaa caacatcagc cagacaaaga cgttatcgac cgccagcgat
    12901 ttctcaatca gatagcccgt gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct
    12961 tcagtttgca ccagatacca ccagaaagcg gcgttaaaca gtaacgacag cgtcacccag
    13021 accagcgacc aggccgcagc ctgtttcatg gtcatggcat gtgccccacg acgcccctgc
    13081 aacaacaggt cgatagccag cataatggcg acaacaacag cgaatccgcc ccatagcaac
    13141 ggcgtgccga cagtattcat agaagttcct tacacataaa aaaacggcca acgtcggaag
    13201 acgtcagccg ctgcttttta tgcatagacc tcgccttccg gcaaggtctc acttacaaca
    13261 aaaaaggaat acgtctgcgt attcctttcg ggttgcccgg tgaccggatg tggtttttca
    13321 cacatcgtaa tgacgatcga ccggcaatga agttactccc ctttgcaggt aacaaagtat
    13381 gacagaccat tcagccagtc aatggtctgt tacatacctt ttacactgtt ttactttggc
    13441 aattatagtt ttacgctatc tgccggaaaa atcaccccag tctggcggcg gatctcggtc
    13501 agcagtttgg cggtgatgcg actgaccgcc agccccggat gatccaccag atgctcatcc
    13561 accagggtag cgaacagctc tgcttcgtag agcatggtat taatatgctg cggctgggtg
    13621 agatcctgca tttggctgcc acgcggcacg aagcacactt tctggcattc agacagtttt
    13681 tcaatcacca gcgatcctgc ttcgccctga atctcgctcg ccaggacaga atcactgact
    13741 tttgagtgct gcaaggtgac gctgaaatca ccgtaatcca tcaccaccac gccgtgggca
    13801 tctacaccgc ttgccagcaa actggcggtt gcctgcacgc ttttcggctc accaaataac
    13861 gccaccgccg acgccaggca gtaaaagccg atatccataa ttgaaccgtt agagaatgac
    13921 ggattaaagg tgttgggatt ttcaccgtcc aggtaacgtt gatagcgcga ggaatactgg
    13981 caatagttga aaaagacttt acgcaatttg ccgactttcg gcagcgcctg gcgcagcaaa
    14041 tgaaagttcg gcaggcaggc ggttttaaat gcctcaaaca gcaccacctg attttcccgc
    14101 gcacaggcaa tggcggcatc cacttccgcc agattcgacg ccagtggttt ctcgcaaatc
    14161 acatgaattt tatggctaag gaaaagttgt gtctgggaaa agtgcagggc attcgggctg
    14221 gcaatataca ccgcgtcaat ggcatcgctt tccgccatcg cttccagcga ggtaaacaga
    14281 tgctcgacag aaaaatcatt ggcgaagtgc tgagcctgct caaggctgcg ggaatatacg
    14341 gcggttaact tgtatttacc gctctcatgg gcggcctcga cgaactggcg agtgatccag
    14401 ttcgtaccaa tcacagcgaa acgtatcata acgcgtggat actccataaa gggattctgt
    14461 cgtcaatgta gcacgccttt ttcatcttcg acgtgcgcag atacgcatta ttcttttgcc
    14521 ggacagccat gctcactaaa cggcccaatc atgaccgatg gcccgagata tttcggctgg
    14581 cgatggagga ttttggcgtc aatcacggcg ctgacacgag cgagtttctc ccgcagttga
    14641 gtataaaaac cgtgcttcat attgaggtca ggcgcgttaa aaccgatagc gtcgatgccg
    14701 tactgttttg ccagccagat ggcgcgttga ttatgaaatt cttgcgagat aatggtgata
    14761 tggttttcac caaaaacctt tttggctcgc actaccgaat ccagcgttga gaatccggca
    14821 tagtcgcaga agatcacttt cgccggtacg cctttagcga tcaagcctgc atacctgagg
    14881 cttcatcgta atttttacgt ccgttatcgc cacttaccag tagccatttc acttttccgg
    14941 cgtggtacaa cgctgctgcg gtatcaatac gtcgggtaaa gtagcgatta cccggcctgg
    15001 cccccagcaa taagcccacg ttacgcgccg gaactgcgtt gacatcgctc caggtcagct
    15061 gcttgctggc gttgaccata acgcgatcgg caataaaaat ggtcgctcct gctataagca
    15121 acaacaaaca gccaatctta agagtccggc gagaaaaaca aatacggaga agaaggcggg
    15181 gctgcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc taaagaaaga
    15241 aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa ctgcgttagc
    15301 cacagccggg tgtcgaactc aagctggtgg tactgcggct ccatatggca gcacaactgg
    15361 taaaacgctt tgttatgctc tttctccttc aggtgcgcca gttcgtgcac cacgatcata
    15421 cgtaaaaacg gttccggcgc gttgcgaaac acagtagcga cgcggatctc tgcttttgct
    15481 ttcagcttgc cgccctgcac acgagaaaca gcggtatgta acccgagcgc gttttttagc
    15541 acatggatct tattgtcata catcacttta ttgatcggcg gggcattgcg cagaaactga
    15601 ttttttagat ccggagtata ttgccagagg gctttatcgg tggcgtagtc gtgtgttccc
    15661 ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagcgt tcgcacctgg
    15721 gaaagtagct gctcgggata accctgaaga taagtaagat tgctcataac cgcccacgaa
    15781 aaagagaaaa agggtatact cacgcaccct tttcaggggc aacgtcgaaa ttttatcatt
    15841 caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta caacgttttc
    15901 cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcggatgaa tatttgctgc
    15961 aacagttgga cgacacagaa atccgcggcc cggtgttgat cctgaatgat gcctttggtg
    16021 cgttaagctg cgcgctggcg gaacataagc cgtacagcat tagcgactca tacatcagtg
    16081 aactggcgac gcgcgagaat ttacgcctca acgggattga tgagtcgagc gtgaagtttc
    16141 tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa gtgccgaaaa
    16201 cactggcatt gctggaacaa caactgcgtg cgctgcgcaa agtggtcacg tcggatacac
    16261 gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggaa ctgttcgaaa
    16321 aagtgctcgg cccgaccacc accacgctgg catggaaaaa agcgcgcctg attaattgca
    16381 cgttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag ctggaaggta
    16441 ctgactggac tatccataac catgcgaatg tcttctcccg caccgggctt gatatcggcg
    16501 cgcgcttctt tatgcaacat ctgccagaga atctcgaagg ggagattgtc gatctcggtt
    16561 gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa cccgcaggcg aaagtggtgt
    16621 ttgtcgatga atcgccgatg gcggttgctt ccagccgttt gaacgttgaa accaacatgc
    16681 cagaggcgtt ggatcgcagc gagtttatga tcaacaacgc gctctccggc gtggagcctt
    16741 tccgctttaa tgctgtgctc tgcaacccgc cgtttcacca acaacatgcg ctgaccgata
    16801 acgtcgcctg ggagatgttc caccatgccc gccgctgcct gaaaatcaac ggcgagctgt
    16861 atattgttgc caaccgtcac ctggattact tccataaact gaagaagatt ttcggcaact
    16921 gcaccaccat cgccacgaat aataaatttg tggtgctgaa agcagtgaag ctggggcgtc
    16981 gtcggtaaga ttgggagtta cctgccggat gcggcgttaa cgccttatcc ggcctacgtt
    17041 cagcacgtta ctcccaggcc ggataagacg cggcaagcgt cgcatccggc aatgtctgcc
    17101 cagttctgga atcgcgcttc cataaaacaa aaaatccctg tcaccatcct ctacattctc
    17161 ttgtttagcg ttgttcaacg tttatgctac atacgtgaag ttatgacgca taaagaatac
    17221 gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct
    17281 tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg
    17341 aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc
    17401 ccttgctgga tctggtgtgt gcaaaaataa ccgcgtggga agaatcagcg cccgaatttg
    17461 cggaatttaa tgccatggct caagccatgc ctggcggtat agccgtgatt cgtaccctta
    17521 tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt
    17581 cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc
    17641 gattcggcat ttctcccgcc ttg
//
LOCUS       DAZKGS010000092        17430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKGS010000092 DAZKGS010000000
VERSION     DAZKGS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..457
                     /gene="bssS"
                     /locus_tag="ACHOQY_003053"
     CDS             203..457
                     /gene="bssS"
                     /locus_tag="ACHOQY_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9679106.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            572..1690
                     /gene="solA"
                     /locus_tag="ACHOQY_003054"
     CDS             572..1690
                     /gene="solA"
                     /locus_tag="ACHOQY_003054"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9679107.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1738..1851
                     /locus_tag="ACHOQY_003055"
     CDS             1738..1851
                     /locus_tag="ACHOQY_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9679108.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2112..2678
                     /gene="yceJ"
                     /locus_tag="ACHOQY_003056"
     CDS             2112..2678
                     /gene="yceJ"
                     /locus_tag="ACHOQY_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9679109.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2682..3257
                     /gene="yceI"
                     /locus_tag="ACHOQY_003057"
     CDS             2682..3257
                     /gene="yceI"
                     /locus_tag="ACHOQY_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9679110.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3299..4351)
                     /gene="trhO"
                     /locus_tag="ACHOQY_003058"
     CDS             complement(3299..4351)
                     /gene="trhO"
                     /locus_tag="ACHOQY_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9679111.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4576..5496
                     /gene="lpxL"
                     /locus_tag="ACHOQY_003059"
     CDS             4576..5496
                     /gene="lpxL"
                     /locus_tag="ACHOQY_003059"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9679112.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5668..6894
                     /gene="mdtG"
                     /locus_tag="ACHOQY_003060"
     CDS             5668..6894
                     /gene="mdtG"
                     /locus_tag="ACHOQY_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP9679113.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6977..7351
                     /gene="msyB"
                     /locus_tag="ACHOQY_003061"
     CDS             6977..7351
                     /gene="msyB"
                     /locus_tag="ACHOQY_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9679114.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7352..7579)
                     /gene="yceK"
                     /locus_tag="ACHOQY_003062"
     CDS             complement(7352..7579)
                     /gene="yceK"
                     /locus_tag="ACHOQY_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9679115.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7752..10295)
                     /gene="mdoH"
                     /locus_tag="ACHOQY_003063"
     CDS             complement(7752..10295)
                     /gene="mdoH"
                     /locus_tag="ACHOQY_003063"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9679116.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10288..11823)
                     /gene="mdoG"
                     /locus_tag="ACHOQY_003064"
     CDS             complement(10288..11823)
                     /gene="mdoG"
                     /locus_tag="ACHOQY_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9679117.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12217..13374
                     /gene="mdoC"
                     /locus_tag="ACHOQY_003065"
     CDS             12217..13374
                     /gene="mdoC"
                     /locus_tag="ACHOQY_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9679118.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13382..14863)
                     /gene="clsC"
                     /locus_tag="ACHOQY_003066"
     CDS             complement(13382..14863)
                     /gene="clsC"
                     /locus_tag="ACHOQY_003066"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9679119.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14805..15338)
                     /gene="ymdB"
                     /locus_tag="ACHOQY_003067"
     CDS             complement(14805..15338)
                     /gene="ymdB"
                     /locus_tag="ACHOQY_003067"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9679120.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15433..15744)
                     /gene="ymdA"
                     /locus_tag="ACHOQY_003068"
     CDS             complement(15433..15744)
                     /gene="ymdA"
                     /locus_tag="ACHOQY_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9679121.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15865..16197)
                     /gene="csgC"
                     /locus_tag="ACHOQY_003069"
     CDS             complement(15865..16197)
                     /gene="csgC"
                     /locus_tag="ACHOQY_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9679122.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16256..16711)
                     /gene="csgA"
                     /locus_tag="ACHOQY_003070"
     CDS             complement(16256..16711)
                     /gene="csgA"
                     /locus_tag="ACHOQY_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9679123.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16752..17207)
                     /gene="csgB"
                     /locus_tag="ACHOQY_003071"
     CDS             complement(16752..17207)
                     /gene="csgB"
                     /locus_tag="ACHOQY_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9679124.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4369 a         4429 c         4128 g         4504 t
ORIGIN      
        1 aaataattgc acaatatgct aaaaaaatat gaatgacatg ctgttttatt gttcatataa
       61 atcttaaaag cagaaattta tctgttcccc cctttaaaaa ttcggtgaat acccttactt
      121 attggtatac tgaagttgct tcagataaaa acgcattgaa cctcgaataa cgttgtctag
      181 taacacgaat tagggggcca tgatggaaaa aaataatgaa gtcattcaga ctcatccgct
      241 cgtagggtgg gacatcagca ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta
      301 ccagacccca aataagtccg agctggaagg gactgaagtt ggtcagacgc tctggttaac
      361 cactgatgtt gccagacaat ttatttcgat attagaagca ggaatcgcca aaattgaatc
      421 cggtgatttc ccggtaaacg agtatcggcg tcattaacat gacatgacca tccacccaaa
      481 aggcacctca ccggtgcctt taccatttct gggttgtata tctgcattca gttaattact
      541 ctgcttaaac catgagagca gagagaaaaa aatgaaatac gatctcatca ttattggcag
      601 cggttccgta ggcgctgccg ccgggtatta tgcaacccgc gccggtttaa acgtgctaat
      661 gaccgacgcc catatgccac cgcatcaaca cggcagccac catggcgata cgcgattaat
      721 tcgccatgct tatggtgaag gcgaaaagta tgtcccgctg gtcctccgcg cgcaaacgct
      781 gtgggatgaa ctttcccgcc acaacgaaga tgatcccatt tttgtacgct ctggtgtcat
      841 taacctcggc ccggctgact ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg
      901 gcaactcaac gttgaaaagc tcgatgcgca agggattatg gcccgctggc cagaaatacg
      961 cgtcccggac aactacatcg gcttatttga gactgattcc ggttttttgc gcagcgaact
     1021 ggcgattaaa acctggatcc aactggcgaa ggaagcgggc tgtgcgcaac tgttcaactg
     1081 cccggtcacc gtaattcgtc atgacgatga tggcgtaact attgaaacgg ttgacggtga
     1141 gtatcaggcg aaaaaagcga ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga
     1201 gctgcctgtc cagcccgtac gtaaagtatt tgcctggtat caggccgatg gccgctatag
     1261 cgtgaagaat aaattcccgg cgtttaccgg tgaactgccc aatggcgatc aatattatgg
     1321 ttttccggca gaaaacgacg cgttgaagat tggcaaacat aacggaggcc aggttatcca
     1381 ttcagcggat gaacgtgttc cgtttgcgga agtggtcagc gatggttcgg aagccttccc
     1441 gttcttgcgc aatgtattgc cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta
     1501 tgataattcg cctgacgaag attttattat cgatacccta cccggccacg ataatacact
     1561 gctcattacc ggcctgagtg ggcacggttt taaatttgcg tcagttttag gggaaatagc
     1621 tgccgatttt gcgcaagaca aaaaaagcga ttttgatttg acgccattca ggctttcccg
     1681 cttccaataa tcatcattac ggcctccaga atgaggccgt ttttacagga gtatttaatg
     1741 cgtcggctgt tgcactatct catcaataat attcgcgagc atctgatgct ttatcttttt
     1801 ctctggggat tgttggcaat tatggatctg atctatgtgt tttattttta gttttccgca
     1861 ctgacataaa ctgacaaata tttaatctcc ctttcatttg gcgtgaaaaa ttgaatgaga
     1921 ttattcctgc ttctacagca ctcaccagac atctctgatg ctgaacccac taaacttatg
     1981 aagattcttt cattattaat tcaaaattat tgaaataaaa ataaatatca ttcaatttgt
     2041 tatttttgtg ttgcaattca ttctccgaca cgccattttc tttctctcgc aaattcgtat
     2101 tggatgcact tatgtcattc acaaataccc ctgaacgcta tggtgttata tctgcggcct
     2161 tccattggct aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca
     2221 cgctcagtta ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta
     2281 ttttgttaat gatggggctg gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg
     2341 gtccgctgcc gagttattcg cccatgactc gtcttgccgc aaaggctgga catctcgcat
     2401 tgtacctatt gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta
     2461 aaccgatcag cgtttttggc tggtttgacg tccccgcgac ccttgccgac gctggcgcac
     2521 aggcagactt tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtactgtccg
     2581 ttatgcacgg atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc
     2641 gcatgctggg aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg
     2701 gtttaacctt cgcgtccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa
     2761 ttgacaaaga aggtcagcac gcctttgtta acttccgcat ccagcatctt ggctatagct
     2821 ggttatatgg cacctttaaa gatttcgacg gtacttttac ctttgacgaa aaaaatccgg
     2881 ctgccgataa agtgaatgtg acaattaaca ccaccagcgt cgatactaat cacgccgaac
     2941 gcgataaaca tcttcgcagt gcagatttcc tcaataccgc aaaatatcca caggcaacat
     3001 tcacctccac cagcgtgaag aaagacggtg acgaactgga tattaccggc gatctgacgc
     3061 tgaatggcgt aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc
     3121 catggggtgg taaacgtgca ggcttcgagg ccgaaggcaa aattaagctc aaggacttca
     3181 atatcaagac agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag
     3241 gcgtacagca gaagtaagcg tgaaatgttg ccggatggca cgcatccggc aatgatattt
     3301 attccgttgg atcaggaatg cccagtgttg tattcagacg tccacgagac ttattaaaga
     3361 tcttattgcc attttcacgt cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc
     3421 tttcttcgca gcaaatctca ctacaacaac ctttgtattt ttccgcacat actggacact
     3481 gaataaacag caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcgc
     3541 cgcactggtg gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa
     3601 aaacaaaatt tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt
     3661 attcaataat tccgccctcg atatgccaga ctttgttgaa tccgttatgt ttcatccagg
     3721 cactggcctt ttcacaacga atgccgccgg tgcagtacat gacgattttt ttatctttat
     3781 gtgcctgcat catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt
     3841 cgagcgcgtt ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaata
     3901 gtgcatcggg atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcgccaacat
     3961 tgctggcatc aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca
     4021 tgcgcagtac ccagaaagat ttcccgtcat catccaacgc gatattcagg cgtaaaccct
     4081 ctaaagccgg gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta
     4141 cgctgatttg cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acattcagcg
     4201 cggtaaacag ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact
     4261 tgtaaaacga aatggtggtt cgcggttcgc tctcagccaa cattttggct tttagcgcgt
     4321 cgttggaaat gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt
     4381 aaaaatccgc ggcatgatat agcaattatc gataattaac atccacacat tttacgctac
     4441 atttgcgcat taaaaattat ttgttattta caagtgcggc aatttcgccc agtcttcagc
     4501 cacaaatttt ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gaataggttg
     4561 aaaaacagga ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt
     4621 tattggttaa cctggttggg tattggcgta ctttggttag tcgtgcaatt gccctacccg
     4681 gttatctacc gcctcggttg tggattagga aaactggcgt tacgttttat gaaacgacgc
     4741 gcaaaaattg tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc
     4801 cgtaaaatgg tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga aaccggcatg
     4861 gcgtggttct ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg catggaacac
     4921 attcgtgacg tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca
     4981 ctggagctgg gtgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg
     5041 aacgataatc cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg
     5101 atgctcgacc gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc
     5161 tggtacgcac cggatcatga ttacggcccg cgctcaagcg ttttcgtccc gttatttgcc
     5221 gttgagcagg ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt
     5281 ctggtgccct tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt gattatgctg
     5341 ccgccagagt gttctccgcc actggatgat gccgaaacta ccgccgcgtg gatgaacaaa
     5401 gtggtcgaaa aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt
     5461 aaaacacgcc cggaaggcgt tccttcacgc tattaaatct cccctgccgg atgcttcaga
     5521 atggcatccg gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt
     5581 gcgccccctg gaagtcgagc gcataattag tgtgcttatc ttttcttctt atgttcaccg
     5641 cgcctggcgc accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac
     5701 tggaaacgaa acctgatcgt cgcctggcta ggctgttttc ttaccggtgc cgccttcagt
     5761 ctggtaatgc ccttcttacc cctctacgtt gagcagcttg gcgttaccgg tcactccgcc
     5821 ctgaatatgt ggtccggtat tgtcttcagc attacatttt tattttcggc catcgcctca
     5881 ccgttttggg gtggactcgc cgaccgtaaa ggccgaaaac tcatgctatt acgctctgcc
     5941 ctcggcatgg gcatcgtgat ggtgttgatg ggactggcac aaaatatctg gcagtttttg
     6001 atcctacggg cgcttcttgg gttacttggc ggatttgtcc ccaacgctaa tgctcttatc
     6061 gccacacaag taccgcgtaa taaaagcggc tgggcgctgg gtacgctctc cacaggcggc
     6121 gttagtggtg cgttgctcgg cccaatggct ggcggcctgc tcgccgatag ctacggctta
     6181 cgtccggtat tctttattac cgccagtgtg ctcatactct gctttttcgt caccctgttc
     6241 tgcatcagag aaaaattcca gccggtcagc aaaaaagaga tgctgcacat gcgggaagtg
     6301 gtgacatcac ttaaaaaccc gaaactggta ctcagcctgt ttgtcaccac gttaatcatc
     6361 caggtggcga caggctcaat tgcccccatt ctgacgctgt atgtccgcga actggcgggt
     6421 aacgtcagta acgtcgcctt tatcagtggc atgatcgcct cggtgccagg cgtggcggct
     6481 ctgctaagcg caccacgact cggcaaactt ggcgatcgaa tcggacccga aaagatcctg
     6541 attacagcgc tgatcttttc tgtactgctg ttgatcccaa tgtcttacgt tcagacgcca
     6601 ttgcaacttg ggattttacg ttttttgctc ggtgccgccg atggtgcact actccccgcc
     6661 gtacagacac tgttggttta caactcgagc aaccagatcg ccgggcgtat cttcagctat
     6721 aaccaatcgt ttcgtgatat tggcaacgtt accggaccat tgatgggagc agcgatttca
     6781 gcgaactacg gtttcagagc ggtatttctc gtcaccgctg gcgtagtgtt attcaacgca
     6841 gtctattcat ggaacagtct acgtcgtcgt cgaatacccc aggtatcgaa ctgatttttc
     6901 gcctttcata cttgcaaaag cggagaatca gctatccttt tccctgaaac ctcatcaact
     6961 caaagggaga atcgtgatga ccatgtacgc aacgcttgaa gaagccattg acgctgcacg
     7021 cgaagaattt cttgcagaca accccggcat cgacgccgaa gatgcgaatg tgcaacagtt
     7081 caatgcccaa aaatacgttt tgcaggacgg cgacatcatg tggcaagttg agttttttgc
     7141 cgacgaaggg gaagaaggtg aatgtttacc tatgcttagc ggtgaagccg cgcaaagtgt
     7201 ttttgatggc gactatgatg agatagagat acgccaggag tggcaggaag agaatacatt
     7261 acatgaatgg gacgaggggg aatttcagct tgagccaccg ctggataccg aggaaggacg
     7321 cgcagcagct gatgagtggg atgaacgtta atcactcata cgggccatga tgaatgtcga
     7381 tcggcagcag taaagtatca aaaaccagag agaatggcag atcaaggatc gtgacataac
     7441 gccaggcgga gtcacgcaca tcccattgca caccgggata atattggttg ccatgcccct
     7501 gccccggtat agtgcgacta ataatgctgc cacagccact cagcaaggtc accatgatgc
     7561 tcaccacaat taaacgcatt tgatacctct ttcgtaaaaa aataccggca taacgccggt
     7621 atttttattg gctcgttgtt tttgccggat gcggcgtgaa cgccttatcc gacccacaaa
     7681 atcttgcaaa atcaataaat tgcaggaacg atgtaggcct gataagcgta gcgcatcagg
     7741 caactacgtt tttattgcga agccgcatcc ggtttacgca atgccagtgg attgagcttt
     7801 atcccttcgt aataactcac ccatgaagaa tatctctccg gggaattcca gacgcggaaa
     7861 tgcagacggg ccatcgtcac cggatcgctt agcagcacca ggcgacgatc gcgattcagc
     7921 ttctctggcg tctcgttcag cgcctgttca acgtggcggt cacgggcgat ttccagcacc
     7981 ttactggcgc ggtgacgcgc ggtcgccatt gcggttgcca gagcgttaaa tgacgggtta
     8041 aacactgcgt gcataaagcc atcatcaagg gagcgttgac gattcatctc aaggaaccga
     8101 tcggtatcaa ccagcacctg cggcggcgaa tactcttccg ggatcaggaa cagtttccag
     8161 cgtttggtgc gcagaccaac ggtggcacgg ctggaaatca ccgaaacaaa cggtgacagg
     8221 atcaacgaga agacaatcgg tgccagccag aacaggaaac gcagatccaa ccacgccata
     8281 ccaacagccc acactaaccc taacagcagc tgtgagccgt ggcgtttgaa cgcttcaccc
     8341 caggaagtgg agtcatcatc acgctgcggt gaattccaca ccacttccca gccaaggaac
     8401 gcgctgacga cgaagaccgt atggaacagc atgcgtaccg gagccagcag cacggaaaac
     8461 agcacttcca gcagcaacga taatgtaacg cgccagaagc cgccgtattc tttcgttcct
     8521 ttgcaccaga taagcaaaat gctcaataac ttcggcagga acaacagcac catggtcgaa
     8581 gcaaaaagtg caatcgccag ctcaggacgc cactgcggcc acaccgggaa caactgccgt
     8641 ggttgcagga agtattgcgg ttcggtcaac gcatgtacta cctgcaatgc agtagagagc
     8701 gcgaggaaca taaaccacag cggagcggag agataagaca tcacgcccgt caggaatacc
     8761 gcacggtgaa ccgggtgcat acccttcacc aggaacagac ggaagttcat caggttaccg
     8821 tggcaccagc ggcggtcacg ttttagctca tcaagcaagt taggcggcaa ttcttcataa
     8881 gaacccggga gatcgtaagc aatccagacc ccccaacctg cacggcgcat caacgccgct
     8941 tccacgaagt catgtgacag gattgaaccg gcaaaggaac cttcgcccgg cagcggagcc
     9001 agtgcgcagt gctcgataaa cggtttcacg cggataatcg cgttatgtcc ccagtagtgc
     9061 gactcgccaa gttgccagaa gtgcaaaccg gctgtaaaca gtggcccata cacgcgggtc
     9121 gcgaactgct gacagcgcgc atacagcgta tccataccgg acgctttcgg cgacgactga
     9181 atgatcccgg cgttcgggtt ggcttccatc aggcgcacca ggccgcataa acaatcaccg
     9241 gtcattaccg agtcagcatc cagcaccacc atgtagctgt actggctgcc ccagcgacgg
     9301 cagaagtcat cgatattacc gcttttacgc ttcacgcggc gacggcggcg gcgatagaaa
     9361 atctgacctt cgccaccgac ttcagcgata agctccatcc aggctttttg ctctgcgacg
     9421 cagatatccg ggttataact gtcactaaga atgtagacat caaagtgttt ggcattcccg
     9481 gtggctttta ctgattccca cgttgcacgc aggccagcaa aaacacggtt cacgtcttcg
     9541 ttacagatag gcatgatcaa cgccgtgcga tgctccgggt ttaatggttc atcgccaact
     9601 gttgacgcag atatactgta tttatcgcga ccaataagca gttgcaggaa gcccattaac
     9661 gccgtccaga atccggcgga cacccaacag aacagtaccg caaagaggat caggataccg
     9721 gtttgcagca tataaggcag aagctgcata aaggaaaccc acaaatcctg accaaccata
     9781 tccataggat taatcagcgc ccaaccctga taaggaagaa tggtcttcat ataccaggtc
     9841 gcgacgacag tttgcgcgag cgtcaggatc aacagaatgt aacggcggat ggtaccgacg
     9901 gtacgccact tttgctcgct ctcctgctct tctttggtca aacgagccag atagcgcggc
     9961 gtgacatcgc gtccacgcag gcgatcccag aaacggccta ccgggttggt acgccacggg
    10021 tcgggaaaca tcgaggagcg ttttgcttct ggcatcgcct tcagctgatc gcgcccttcg
    10081 tcgtctttaa ttaactgtcc atcagcaagt gaatctggcc aggcttgttc cagacgcgcc
    10141 tttaccgagc cttgcgggga atcatcctcc cgcgcccagg tgcggtgttc ggcatccagc
    10201 gcctgatgaa cggcgcggat atcagtcttc ggcaatgccg ctttctcgct tgcggcgatg
    10261 ggcattgcgt caatgtactc agttgtctta ttcattggca ggtaactggt agctccaggt
    10321 ttcactcaac gtctgatcgg cattcaccag cgcagcacgc atttcagtgg ttttcttggc
    10381 atctttcact ttcacacgca tcaccagacg ccagcctttg gtaaccgggt tatagcgcac
    10441 cgtgctttca actatctcac cattatcacc aatgctggtt tgcgctgtga ccggggtatc
    10501 ctctggcagt tttttcatct ctgcgccggt aaaatcgacc acaaaggcga tagtaccgtc
    10561 aggctggcga atcaggttcg actgcttcac atcccccgtt gaacgacgcg tttgttgcac
    10621 ccatgcgtta tctggcgcat gcagtttgtc ttcatcacgg ctgaaggtga tggtgtattt
    10681 aaagttcatc tctttacccg gctccggcag ctgatccggc gtccagtaag cgacgatgtt
    10741 atcgttggtt tcatcgttgg ttggaatttc caccagctca acgctgcctt taccccactc
    10801 ccctttcgga gtcacccatg cgcttggacg aagatcgtaa cgatcatcga gatcttcaaa
    10861 gcgggagaaa tcacgaccgc gctgcaacag accgaagcct tgcgggttct ccatcgagaa
    10921 gctgctgacc gctaaatgtt tcgggttatt caacggacgc cagatccatt cgccattacc
    10981 ggcatggata gacagaccgt tagagtcgtg caactccgga cgatagttat ttgcaggcga
    11041 cggttggttc ggcccaaaca ggaacatact ggttaacggt gcaaccccca gtttgccgac
    11101 tttatcgcgc agatagattt tcgactgcac atccacaacc gtgtcacgcc ctggcataac
    11161 cacgaatttg taagcacctg tcgcgcgcgg cgagtcaagc aatgcataaa tggttaaacg
    11221 tttatcagtc ggttttggac gctcgatcca gaactctttg aagcgtggaa attcttcacc
    11281 cgatggcaag gcggtatcaa ttgccaggcc gcgggcagaa aggccataaa cctgacctgc
    11341 accaatcacg cggaaatagc tggccccgag catgctgacg atttcatcgt ttttatcttt
    11401 gctgttgatc gggtaaagca ctttaaaacc ggcaaaacca aggtctttta ccgtgtcttt
    11461 gtcatgctga acatcgccga aagtgaaata atccgggctg tatttgattc gtttgactgc
    11521 ggtggcagtc acttcattta ttttgaccgg ggtatcgaag tacataccct gatggtagaa
    11581 ctcgagtttg aatggggtct tcagattgtt ccagtacgct ttgtcatgat taaactggat
    11641 ctgctgatag tccgcgtatt tcatatcgcg gaaaacggag ggcaagttgc ttttgggcgc
    11701 ctcatagcct ttcccggcta aggattgagc ttgctttgcg acatcatcaa tactgaaagc
    11761 ccagcttgaa gatgtataca gggttaacat tactgcagca ctcaaccaac gcattttcat
    11821 catttgtagt ttatgtttca taattagtaa gcacttcccc ctttgtgtgc ttatatcgat
    11881 ccgatctatt ttaatggata accagccatt ccgacaacag aatcccggta tcgttcagca
    11941 cgctggctca tggattaagc acctgattgc aaccgttttc actttccgtg cttctcctaa
    12001 agacagaagc atcgacttcg tgtagggttt gccagcaatt ttatttatca accgtcttac
    12061 agacgcttcc attctggtta gtggtagcga tgatgctatg cataaaaacc ttgaagcctg
    12121 cgcctggcgc ggtcaggtcg gtgtcgctat ggcaacaagc ctaaaggcat aacccgacag
    12181 ggataagacg aaaaatccga gattagttaa ccatatatga atccagtacc cgcgcaacgt
    12241 gaatatttcc tcgactccat ccgcgcctgg ctgatgttgt tagggatccc ttttcatatt
    12301 tctttaatct attcgagcca tacatggcat gtgaatagcg ccgaaccatc attgtggctg
    12361 acccttttta atgacttcat ccactcgttc cgcatgcagg tatttttcgt tatatccggc
    12421 tacttttcct acatgctttt tttacgctat cccttgaaaa aatggtggaa agtacgtgtc
    12481 gaacgtgtag gtatcccgat gttaacagcc atccccctac tgacattacc gcaatttatt
    12541 atgctgcaat atgtcaaagg aaaagcggaa agttggcctg ggctgtcatt gtatgacaaa
    12601 tataatacgt tggcctggga attaatatca cacctgtggt ttttactggt gttagtagtc
    12661 atgacgacgc tgtgcgtatg gatatttaag cgcatcagaa ataatttaga aaattctgat
    12721 aaaacgaata aaaaattctc gatggtaaaa ctatcggtga tttttttatg cctcggcatc
    12781 ggttatgcgg taataagaag aacgattttt attgtgtatc cgcccattct gagtaatggc
    12841 atgttcaatt ttattgtcat gcaaacgctg ttttatttgc cgttctttat cctcggcgca
    12901 ctggctttca ttttccctca tcttaaagcc ttgtttacca cgccgtctcg tggctgtacc
    12961 cttgcagcag cattggcgtt tgtcgcttat ttactcaacc agcgctatgg cagtggcgat
    13021 gcctggatgt acgaaaccga gtcggtgatc accatggtcc tcggtctgtg gatggtgaat
    13081 gtggtcttct cctttggcca ccgtttgctt aacttccagt cagcgcgggt gacttatttt
    13141 gttaacgcat cgctgtttat ctatctggtt caccacccgt taacgctgtt tttcggcgca
    13201 tacattacac cgcacatcac ctccaactgg cttggttttc tctgtggcct gatatttgta
    13261 gtagggattg cgataattct gtatgaaatt catttgcgca tcccgttact gaagtttttg
    13321 ttctctggta aaccggttgt taagcgtgag aacgataaag caccagcccg ttaagccaca
    13381 tttacaataa ccattccacg ggcaatatcg acgccagtct gaccataacc cgcttccaga
    13441 aactggtggc gggttctttt ttgagaataa tctctttctt tgcatgacga tcaacccagt
    13501 tgatccgtcc ccacctgtcc agacggagct gccaggccgc atcatactgg ctctgaataa
    13561 agcgtttatc aattaactgt gccagcgttt cgctctctat cacgaagccc atttcagtat
    13621 tgagcaatgt tgaacgcgga tcgaaattga aagagccgat aaacaccgtt ttaccatcga
    13681 tgctaaaggt tttagcatgc aggctggctc cggaattacc ggttatgccg cgatcgtgta
    13741 acgtactact ttgttcacgc gtcggcttga gttcatataa ttccacgcca tagcggagca
    13801 attttttgcg ccagcgcgcg tatccggcat ggacgacagc aacatcgtta gcggcaagag
    13861 aattggttag gatcgcaatc tttacccctt ttctcaccat ccgtaagagt tgcgccacac
    13921 ctgcgcgtgt cggtacaaaa taggaagaga taatatcgat gcgttcactg ggtgagccca
    13981 tgatatcgaa caggcgctgc ggtaacagtg aatgccgttt tgccttgccc tcccctttcg
    14041 ccggatcatc acttaataaa cgtgttttcg cccagataag cggcaacgtt ccatcaacca
    14101 gatgatttat aaacggactg gattccattt tgcgtaagta acgatgcgtc atagcatcgt
    14161 tatgccagga ggcgggtaac tcgatgcgat ccgccatttc accttccggg acatccagca
    14221 cttgctgtaa gggtgaaacc gatttgcaat accagtagcg ggcgaaatca tcggcaacgt
    14281 cctctaccac gggccctatt gccataacat ctaaatccga aaaaagtggc tcttcccctg
    14341 ccccaaaata ggcatcacca atatttcgcc cccctaccag cgtcaccacg ccatcgacag
    14401 tgaaactttt attgtgcata cggcgattga gacgggaaaa gtcggtgata taaccaagcg
    14461 gacgcagcaa gcgaaacgag aaaggattaa aaagccggac ttcaatgcgg ggatggctgt
    14521 caagcaagcg taaaatgtcg tcaagtccgg gcgtattgtt gtcgtccagc aacaaacgga
    14581 cgcgaacgcc acgctttgct gcggctaaca gggcggaaaa cagtaaccga cccgacatat
    14641 cgtcctgcca gatgtaatac tgaacatcca gcgtatgctc tgccatttcg gccagacgat
    14701 accgggcggc aaacgcatcc agacttttct ccagagggaa aagaccacac tgaccgggat
    14761 gttgcgaaca cagtggcagc accgcgctcg ccagccgggg caaatcattc atctccttgt
    14821 tgggtaagga gtctttcgta gaggtgggcg ttttcttcat cataacagac aaagtatacc
    14881 tgttcgggta aagcgtgacg ggtaataaat tctgaaacgg ttttaactgc gatttcagcc
    14941 gctgccgcac gagggtaacc ataaacccca gtactgattg caggaaaagc cactgacgta
    15001 tagctgtttg ccgccaccag tcgtaggcta ttgagatagg catcctgcaa aagctggtct
    15061 tcgttttgtt caccaccacg ccagactggc ccgacggtgt gcactacggc tttagcggga
    15121 agagcgcctg caagcgtaat aacggcatgc cccgtagggc aatcgccctg ctgttgcctg
    15181 acttttaaac aggcatccag cagggccgga cccgctgcgc gatgaatggc cccatcgacg
    15241 ccgccgcctc ccattaatga cggattagcc gcattcacaa tcacatcaac ggccagtttg
    15301 gtaatatcac cctgcacaac atgaatacgc gttttcataa cgcctcctta caccttcttg
    15361 tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctgctca gcaacgtgcg
    15421 ttgttcagaa acttagttat attcgatgtt cattacagcc agctttttct gtttatcaag
    15481 atactgcact tttactgagg ctatttgcgc gtttttaaca ttaccgctca ttaacgcttt
    15541 gctggaatag gttttgcccg gaacagaacc attctgtggg cagttcattt ctacgggtga
    15601 tgactgggtg ctgacgtcac atgccggttc cacaatttgg ccataaaaat ggatcacccc
    15661 cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa agcaaactgc cgagcataag
    15721 agagtttaaa aaaggtctga acatttattc tatttcctca atgattagtc atccttgagg
    15781 gttgtgttat ccatactttc aggttggctg attcgcagca gaccattctc tccagattca
    15841 tcttatgctc gatatttcaa caaattaaga cttttctgaa gagggcggcc attgttgtga
    15901 taaatgaagt gactgtccat cagaaacagt aacaactatt ttcacccgat cttccgggga
    15961 aatatttaaa ctcaacttcg tcaaagcaat aggttgatta gcaggcaatg agagggtctt
    16021 ttcttgcttc gtctgacttt gccctgaact gccttcgcgc agggacaata tttgtactct
    16081 gcacagacaa gattgagtaa gagtgacttc aggaataatg gtatacacat ccccttgctg
    16141 ggtcgtatta aaggttatct ggctggaaag tgccgcaagg agtaataacg cattcatatt
    16201 cttctcccga aaaaaaacag ggcttgcgcc ctgtttctgt aatacaaatg atgtattagt
    16261 actgatgagc ggtcgcgttg ttaccaaagc caacctgagt cacgttgacg gaggagttag
    16321 atgcagtctg gtcaactgca gcaccgttgc caccaccgaa ctgtttaaca gtcatttcag
    16381 aatttttgcc gttccactga tcaagagtgg cgctgttacc gaagccacgt tgggtcagat
    16441 cgattgagct gtcatctgag ccctgaccta catctgcacc attaccaccg ccatgctggg
    16501 taatagtcaa gtcagagtta cgggcatcag tttgcagagc aagtgcagag ttaccgccac
    16561 cgtactggta aatgttcagc tctgaatttg ggccgctatt attaccgcca ccaccgtggt
    16621 taccgccgcc gccgtactga ggaacaacac ctgccagagc gctaccggag aatacgattg
    16681 ctgcaattgc tgctactttt aaaagtttca tgtaaaaccc ccatcggatt gatttaaaag
    16741 tcgaatggaa attaacgttg tgtcacgcga atagccattt gcgactgtct ctgcactaca
    16801 attgccgttt tttgagtacc atactgtgta atatttgctt tattaccaga acctttctgg
    16861 ataatcatcg cagtattacc ataagcacct tgcgaaatac tggcatcgtt ggcactgccc
    16921 gcctgatcaa tatatgcaag gttataatct cctgtctggt caatctttgc ccggttgcta
    16981 ctaccttctt gcgcaacaac cgccaaaagt tttgagcctc cctgccgtaa ctgagcacta
    17041 ttattcgtcc cagcttgacc aattatggct gcctgattaa atgaagactt actcaattca
    17101 tttaccgcga agttatattc tgaattagct aaatcataac ctgctgcggc tgcaatccca
    17161 ggcgcaccca gtattgttaa catcataaat aacaatttgt ttttcatgtt gtcaccctgg
    17221 acctggtcgt acatttaaga aattaaatca tttcaacttg gttgttaacg caacctgtat
    17281 tttgttaacg ctgcgttacg atggaaagta tgactgcgga aatattttta ataactcacc
    17341 cgcgcgttgt atttttcttt ttttgttcat accaaagtat taaaacccac gtaaatacgc
    17401 tgttatctac gcaaaaaata ttttgttttc
//
LOCUS       DAZKGS010000093        17229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKGS010000093 DAZKGS010000000
VERSION     DAZKGS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..1794
                     /gene="fdrA"
                     /locus_tag="ACHOQY_003072"
     CDS             127..1794
                     /gene="fdrA"
                     /locus_tag="ACHOQY_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9679125.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1804..3063
                     /gene="ylbE"
                     /locus_tag="ACHOQY_003073"
     CDS             1804..3063
                     /gene="ylbE"
                     /locus_tag="ACHOQY_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9679126.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3074..3889
                     /gene="ylbF"
                     /locus_tag="ACHOQY_003074"
     CDS             3074..3889
                     /gene="ylbF"
                     /locus_tag="ACHOQY_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9679127.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3886..4779
                     /gene="ybcF"
                     /locus_tag="ACHOQY_003075"
     CDS             3886..4779
                     /gene="ybcF"
                     /locus_tag="ACHOQY_003075"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9679128.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(4916..5983)
                     /gene="purK"
                     /locus_tag="ACHOQY_003076"
     CDS             complement(4916..5983)
                     /gene="purK"
                     /locus_tag="ACHOQY_003076"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9679129.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(5980..6489)
                     /gene="purE"
                     /locus_tag="ACHOQY_003077"
     CDS             complement(5980..6489)
                     /gene="purE"
                     /locus_tag="ACHOQY_003077"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9679130.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(6607..7329)
                     /gene="lpxH"
                     /locus_tag="ACHOQY_003078"
     CDS             complement(6607..7329)
                     /gene="lpxH"
                     /locus_tag="ACHOQY_003078"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9679131.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(7332..7826)
                     /gene="ppiB"
                     /locus_tag="ACHOQY_003079"
     CDS             complement(7332..7826)
                     /gene="ppiB"
                     /locus_tag="ACHOQY_003079"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9679132.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8000..9385
                     /gene="cysS"
                     /locus_tag="ACHOQY_003080"
     CDS             8000..9385
                     /gene="cysS"
                     /locus_tag="ACHOQY_003080"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9679133.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(9421..9942)
                     /gene="ybcI"
                     /locus_tag="ACHOQY_003081"
     CDS             complement(9421..9942)
                     /gene="ybcI"
                     /locus_tag="ACHOQY_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9679134.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(10050..10262)
                     /gene="ybcJ"
                     /locus_tag="ACHOQY_003082"
     CDS             complement(10050..10262)
                     /gene="ybcJ"
                     /locus_tag="ACHOQY_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9679135.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(10264..11130)
                     /gene="folD"
                     /locus_tag="ACHOQY_003083"
     CDS             complement(10264..11130)
                     /gene="folD"
                     /locus_tag="ACHOQY_003083"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9679136.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            11611..12153
                     /gene="fimA"
                     /locus_tag="ACHOQY_003084"
     CDS             11611..12153
                     /gene="fimA"
                     /locus_tag="ACHOQY_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9679137.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            12373..13065
                     /gene="fimC"
                     /locus_tag="ACHOQY_003085"
     CDS             12373..13065
                     /gene="fimC"
                     /locus_tag="ACHOQY_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9679138.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            13096..15705
                     /gene="sfmD"
                     /locus_tag="ACHOQY_003086"
     CDS             13096..15705
                     /gene="sfmD"
                     /locus_tag="ACHOQY_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9679139.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            15747..16724
                     /gene="fimH"
                     /locus_tag="ACHOQY_003087"
     CDS             15747..16724
                     /gene="fimH"
                     /locus_tag="ACHOQY_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9679140.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            16735..>17229
                     /gene="sfmF"
                     /locus_tag="ACHOQY_003088"
     CDS             16735..>17229
                     /gene="sfmF"
                     /locus_tag="ACHOQY_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP9679141.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAI"
BASE COUNT         4138 a         4274 c         4642 g         4175 t
ORIGIN      
        1 gaattctttt gttaataatc cctgtgtgat attcatcacc ttatttactc gttgtcatcg
       61 ataccgtaat cgccacatta acactgctcg tgcaattgcc atgggtgcaa tttttaagga
      121 gttgttatga tccacgcctt tattaaaaaa gggtgttttc aggattcggt cagtttaatg
      181 attatttcac gaaaactcag cgaatcagaa aatgttgatg atgtttccgt aatgatgggt
      241 acgcccgcca ataaagcgtt attagatacc acaggtttct ggcatgacga ttttaataac
      301 gccacgccga acgatatttg cgtggcaatt cgtagcgaag cggcggatgc ggggatcgcg
      361 caggcgatta tgcagcagct tgaagaggcg ctaaaacaac tggcgcaggg gtcaggcagc
      421 agccaggcgt tgacgcaggt gcgtcgctgg gacagcgcct gtcagaaatt acccgatgcc
      481 aatctggcgc tgatttcagt ggctggcgag tatgcggcgg agctgacaaa ccaggcgctg
      541 gatcgcaacc tcaacgtgat gatgttctcc gataacgtca cgctggaaga tgaaatccaa
      601 cttaaaaccc gcgcgcggga aaaaggcttg ctggtgatgg ggccggactg cggtacgtcg
      661 atgattgccg gcacaccgct ggcttttgct aacgtgatgc cagaaggcaa tattggcgtc
      721 attggcgctt ccggtaccgg gattcaggag ctgtgttcgc agattgcgct ggcaggggag
      781 ggaattactc acgcgattgg ccttggcggg cgcgacctca gccgtgaagt gggcggcatc
      841 agtgcgctaa cagcgctgga aatgctcagt gcagacgaga aaagcgaagt gctggcattt
      901 gtttcaaaac cacctgccga agctgtgcgt ctgaaaattg ttaatgccat gaaagcaacc
      961 ggcaaaccga cggtggcgct gtttttaggt tataccccgg cggtggcccg cgacgagaat
     1021 gtctggtttg cctcctcgct ggatgaggcc gcacgcctgg cttgcctgct ttcacgcgtc
     1081 acggcgcgac gtaacgcaat agcgcctgtc agcagcggat ttatttgcgg tttgtatacc
     1141 ggcggtacgc ttgctgccga agcggcggga ttacttgccg ggcaccttgg cgtggaagcc
     1201 gacgataccc atcatcacgg catgatgctg gacgccgatg gtcaccagat cattgatctg
     1261 ggcgatgatt tctacaccgt cgggcgtccc catccgatga tcgacccagc cttacgcaac
     1321 cagttaattg ccgatctcgg cgctaaaccg caagtgcgcg tgttgctgct tgatgtcgtg
     1381 attggcttcg gtgcgaccgc cgatcctgct gcctcgctgg tgagcgcctg gcaaaaagcc
     1441 tgtgccgcgc gttcagacag ccaaccgctg tacgccattg ccacggtgac aggtaccgaa
     1501 cgtgacccgc aatgccgctc gcagcaaatc gccacgctgg aagatgcggg gatcgcggta
     1561 gtgagttcgc taccggaagc caccttgctg gcggcagcgt taattcatcc gctctcgtct
     1621 gccacacagc aacacacacc gtcattactg gaaaacgtcg ccgtgattaa catcggatta
     1681 cgcagctttg cgctggagct acaaagcgcc agcaaaccgg ttgtgcatta ccaatggtcg
     1741 ccagtcgccg gcggcaataa aaaactggct cgtttattag aacgtttgca ataaggggtt
     1801 cccatgttta catcagtggc gcaagccaat gctgcggtta tcgaacaaat tcgtcgtgct
     1861 cgtccacact ggctggatgt gcaaccggct tcttcactta tcagcgaact aaacgagggc
     1921 aaaacactgc ttcacgccgg gccgccaatg cgctggcagg agatgaccgg acccatgaaa
     1981 ggggcgtgcg tgggcgcatg tctgttcgaa ggctgggcga aagatgaagc gcaggcgctg
     2041 gcaatactgg agcaggggga agtgaacttc attccttgtc accatgtgaa tgccgtcggt
     2101 ccaatgggcg gtattacttc tgccagtatg ccgatgctgg tggtcgagaa cgtgaccgac
     2161 ggcaaccggg cgtactgcaa cctcaacgaa ggtatcggca aagtgatgcg ttttggcgct
     2221 tacggcgaag atgtcctgac tcgccatcgc tggatgcgcg atgtgttaat gccagtatta
     2281 agcgcggcgc tggggcgcat ggagcgcggt atcgatctca cggcgatgat ggcgcagggc
     2341 attacgatgg gcgatgagtt ccatcaacgc aatattgctt cctctgcact gttaatgcgt
     2401 gcgctggccc cacaaattgc tcgcctcgat catgataaac agcacatcgc cgaagtgatg
     2461 gatttcctca gcgtgaccga tcagttcttc ctcaacctcg cgatggctta ctgcaaggcg
     2521 gcgatggatg ctggcgcgat gatccgcgca ggcagcatcg tcacggcaat gacccgcaac
     2581 ggcaatatgt tcgggattcg ggtaagcggg ctgggcgaac gctggtttac tgcgcctgta
     2641 aacactccgc aaggtctgtt tttcaccggc ttctcgcagg agcaggcgaa cccggatatg
     2701 ggcgatagcg cgattaccga aacctttggt atcggaggtg cggcaatgat cgcagcgcct
     2761 ggcgtaacgc gctttgtcgg tgcgggtggc atggaagcgg ccagagcggt atctgaagag
     2821 atggcggaaa tttaccttga gcgcaatatg cagttgcaga tccccggctg ggatttccag
     2881 ggcgcgtgcc tggggctgga tattcgtcgc gtggtagaaa ccggcattac gccactcatc
     2941 aataccggta tcgcccataa agaggcgggg atcgggcaga ttggcgcagg cactgtgcgg
     3001 gcaccgctgg cgtgttttga acaggcgctg gaagcactgg ctgaaagcat ggggattggt
     3061 tgaggaacgc gcaatgacga tcatccatcc tctgcttgcc agccgtagtg caccgaatta
     3121 tcgccagtcc tggcggttag cgggagtgtg gcgacgggcg attaacctga tgacggaaag
     3181 cggcgaactg ttaacgctgc atcgtcaggg tagtggtttc ggccccggag gatgggtgct
     3241 tcgccgcgcg caattcgatg cgttatgcgg tggattatgc ggcaatgaac gaccacaggt
     3301 tgtggctcaa gggattcgcc tcgggcgttt cacggttaaa cagccacagc gttattgttt
     3361 gctgcgtatt acgccgcctt cgcatcctca accgcttgca gctgcatgga tgcaacgcgc
     3421 ggaggaaacc ggacttttcg gaccactggc gatggcggca agcgatccgc tgcccgctga
     3481 attacgccag tttcgtcact gttttcaggc cgcgctcaat ggcgttaaga ccgactggcg
     3541 gcactggctg ggtaaaggcc ccggattaac gccaagtcat gatgacacgt tgagcggaat
     3601 gctgctggcg gcctggtatt atggcgcttt agatgcgcgc gccggtcgcc cgttttttgc
     3661 ctgttccgac aatcttcaac tcgttaccac agcggtgagc gtcagttatt tacgttatgc
     3721 cgcgcaagga tatttcgcct cgccactcct gcactttgtc catgctctga gttgcccgaa
     3781 gcgtaccgct gttgcgattg attcgctgct ggcgctgggg catacgtcag gggcagatac
     3841 gctgctgggg ttctggcttg gccaacaatt attacaagga aaaccatgaa aacactggtt
     3901 gtagctcttg ggggcaacgc cttactccag cgcggtgagg cgctgacggc agaaaatcaa
     3961 tatcgcaata tcgccagtgc tgtacccgcg ctggcgcgcc tggcccgttc ttatcggctg
     4021 gcgattgttc acggcaatgg gccgcaggtg gggctgctgg cattgcagaa tctggcgtgg
     4081 aaagaggtag atccgtatcc gctggatgtg ctggtggcgg aaagccaggg gatgattggc
     4141 tatatgctgg cgcagagttt gagcgcacag ccgcagatgc cgcctgtcac gacggtgctg
     4201 acgcgcattg aggtttcgcc tgatgatccg gcgtttttgc agccagagaa atttattggt
     4261 ccggtttatc agccagaaga acaaaaggca ctggaagcgg cttacggctg gcagatgaaa
     4321 cgtgatggta aatatttgcg ccgggtggtg gcgtctccgc aaccgcgtaa aattctcgac
     4381 agcgaagcca tcgagttgtt gctcaaagag gggcatgtgg tgatttgcag tggcggcggc
     4441 ggtgtgcctg tgacggaaga tggggcaggg agtgaagcgg tgattgataa agatctcgcc
     4501 gctgcgttgc tcgccgagca gattaatgcc gatggactgg tgatcctcac cgatgctgac
     4561 gcggtttatg aaaactgggg aacgccgcag caacgcgcca ttcgccatgc cacaccggat
     4621 gagttagcgc catttaccaa agccgatggt tcgatggggc cgaaggtaac ggcggtaagt
     4681 ggttatgtca gaagccgtgg taaacccgcg tggattgggg cgttatcgcg aattgaagag
     4741 acgctggcgg gcgaagcggg gacctgtatt tcgctgtagt gatgagagca tccggcaatc
     4801 ggtgcaccat gcctgatgcg acgcttgacg cgtcttatca ggcctacaat gggtaccgga
     4861 gcggtaagcc ggataaggcg tttacgccgc atccggcaat caaatgagca cctgcttaac
     4921 tgaacttact ctgcgcccac atcacgccgc tggcatactc cggcggcagc agcgggatca
     4981 aggcttccag cgtcgcggtc agacgcgatg tgtcgctgtc ggtcaaattc agatgcccca
     5041 ctttacgccc cggacggact tctttgtcgt accagtgcag atgcaccagc ggcagtttca
     5101 gccagtcata attcacatcg ctaccaatca ggttgatcat caccgacgga ctattcacta
     5161 ccggttgcgg taacggcaga tcggtaatcg cccgcagatg cagctcaaac tggctgatgc
     5221 tggcaccgtt ttgtgtccag tgaccgctgt tatgcacacg cggtgccagt tcgttgatca
     5281 gcagaccttg cggggtgaca aaacactcca tcgccatcac gcccacatag cccagctcct
     5341 gcataatcgc cgacagcatc tcttcggctt gcgcttgctg ctgcgcattg gcctgcggaa
     5401 aagcgacgct ggtgcgcaaa ataccgtcct gatgcaggtt atgcgtcagc ggataaaaca
     5461 cggtgctgcc atcaaatccg cgtgcgccaa ccagcgacac ttcaccagag aagttaatgc
     5521 cctgctcgac aatacattcg ccgtaacact ctgccggtaa ctgttcggtt tcattggcac
     5581 gtaaacgcca ttgaccgcga ccgtcatagc caccagtgcg acgcttaaca atcgccagtt
     5641 cacctaaacg ctcaaacacc gcaggccact cgctgcgatc ggcaagtaac tgccacggtg
     5701 cggtcggcag gtggagctta tcgaaaagct gcttctgagt cagacggtcg gcaataatcg
     5761 ggaacacatc gcggttcaca aaggccggat gacgcgccag ctcgcgggtt aatgcggttt
     5821 ccggccagcg ttcgatctca gcggtaatca cgctttgttg aaaaggcacc gccgccggtt
     5881 cagcgtccag cccgaccggc cagacagcaa tgcctaacgg ttcacctgcc tgacgcagca
     5941 tacggcctaa ctgcccgtta ccgaggacgc aaacctgttt catgccgcac ctcgcgggtc
     6001 cgggttttcc agcacttcgt cggtctgggc tttgcgccag tcattcagac gctggtgcag
     6061 ttctttatca tgagtcgcaa gaatttgtgc tgccagtaac gccgcgtttg ccgcgccagc
     6121 tttaccaatc gccagcgtac ccaccggaat gccgcgcggc atttgtacga tggagtagag
     6181 gctatcgaca ccgctcagtg cggcgctctg tactggcacg cccagcactg gcaccagcgt
     6241 tttggcggca atcataccag gcaggtgcgc tgcgccgcct gcgcccgcaa taatcacctg
     6301 ataaccgttc tcttcggcgc tttcggcgaa gctgaacagc ttatcggggg tgcggtgagc
     6361 agaaaccact tcaacgtggt gcgggacatt caggatttcg aagatttcgg cggcgaactg
     6421 catggtagcc cagtcgcttt tggaccccat cacgatggcg acacgcgccg gattattgcg
     6481 ggaagacatg cgtcttaaaa ctcctgtggt gcacaactct cggctttaga gggcacagag
     6541 aatagcacgg aaagagagca aggaaaacgg ttgcgtggct gtgaaatcag caaagttgcg
     6601 ggttttttaa aacggaaaat gaatcagctc aacgtcatcc gccgtgactt tcaccatcga
     6661 accttccgta tgccaggcac cgagtaccac gcgaaaagca ggttgctggt tggcgataag
     6721 ttcatgtacc gccgggcgat gggtatgtcc gtggatcagc cattgcacct gatgtttttc
     6781 catcgcactg actaccgcgt tttggttaac gtccatgatc gccagcgatt tactgctgtt
     6841 ggcttcttta ctgttcgctc gcattcgcgc ggcaatgcgt ttgcgcacaa acaacggcag
     6901 ggcgaggaat agcatttgca gccagggttt gtggactttg gcgcgaaaag cctgataacc
     6961 cgcgtcatcg gtgcacagcg tgtcgccatg cataatcaac acccggcgac cataaagttc
     7021 gagcaccttt tcttccggca ataatgtcat gccgctttca cgggcaaagc gtttgccgag
     7081 cagaaaatca cggttgccat gaatgaaata acagggaacg ccggaatcgg acaccgcttt
     7141 gatcgctgcc gccatctggc gatggagtgg gttgggatcg tcgtcgccaa tccatgcttc
     7201 aaacagatcg ccaagaatat acagcgcgtc ggccttgcgg gcttcccccg ctaaaaaacg
     7261 cagaaaaccg gcggtgatcg ccggttcttc cacgcagaga tgaagatctg caataaagag
     7321 tgtcgccacg attactcgct aacggtcacg ctttcaatga taacgtcttc ttttggcacg
     7381 tcctggtgca taccgctacg accggttgca acacctttga ttttgtctac cacgtccatg
     7441 ccttcaacca cttcagcaaa cacgcagtag ccccaacctt gcaggctttc gccagagaag
     7501 ttcaggaagt cgttatcaac cacgttgatg aagaactgtg cagttgcaga gtgcggagcc
     7561 tgagtacggg ccattgccag cgtaccacgg gtatttttca gaccgttgtt ggcttcgttt
     7621 ttgatcggtt ctttggtggc tttttgtttc atgcccggtt caaaaccgcc gccctgaatc
     7681 ataaagccgt tgataacacg gtggaaaatg gtgttgttgt aaaaaccttc gcggcagtag
     7741 tccaggaagt ttttaactgt ttcaggtgct ttatcgtcaa aagttttgat gacaatatcg
     7801 ccgtgattgg tgtggaaagt aaccattttt gcatcctgtt ccgtttgatt ggtgcttcaa
     7861 cccagttcgg gtcatatata gggtggtgtt atagcataac cgcacgatcg gatcatcacg
     7921 caatgtatgc tgattcgcgc gggaaatatg ggtattatac gcaactcaat tacccacaca
     7981 tgtctaaacg gaatcttcga tgctaaaaat cttcaatact ctgacacgcc aaaaagagga
     8041 atttaagcct attcacgccg gggaagtcgg catgtatgtg tgtggaatca ccgtttacga
     8101 tctctgtcat atcggtcacg ggcgtacctt tgttgctttt gacgtggttg cgcgctatct
     8161 gcgtttcctc ggctataaac tgaagtatgt gcgcaacatt accgatatcg acgacaaaat
     8221 catcaaacgc gccaatgaaa atggcgaaag ctttgtggcg ctggtggatc gcatgatcgc
     8281 cgaaatgcac aaagattttg atgctttgaa cattctgcgc ccggatatgg agccgcgcgc
     8341 gacgcaccat atcgcagaaa ttattgaact cactgaacaa ctgatcgcca aaggtcacgc
     8401 ttatgtggcg gacaacggcg acgtgatgtt cgacgtcccg accgatccga catatggcgt
     8461 gctgtcgcgt caggatctcg accagctgca ggcaggtgcg cgcgttgacg tggtcgacga
     8521 caaacgcaac ccgatggact tcgttctgtg gaagatgtcg aaagagggcg aaccgagctg
     8581 gccgtctccg tggggcgcgg gccgtccagg ctggcacatt gaatgttcgg caatgaactg
     8641 caagcagctg ggtaaccact ttgatatcca cggcggcggt tcagacctga tgttcccgca
     8701 ccacgagaac gaaatcgcgc agtccacctg tgcccatgat ggccagtatg tgaactactg
     8761 gatgcactcg gggatggtga tggttgaccg cgagaagatg tccaaatcgc tgggtaactt
     8821 ctttaccgtg cgcgatgtgc tgaaatacta cgacgcggaa accgtgcgtt acttcctgat
     8881 gtcgggccac tatcgcagcc aactgaacta cagcgaagag aacctgaagc aggcgcgtgc
     8941 ggcgctggag cgcctctaca ttgcgctgcg cggcacagat aaaaccgttg cgcctgccgg
     9001 tggcgaagcg tttgaagcgc gctttattga agcgatggac gacgatttca acaccccgga
     9061 agcctattcc gtgctgtttg atatggcgcg tgaagtgaac cgtctgaaag cagaagatat
     9121 ggcagcggcg aatgcaatgg catctcacct gcgtaaactt tccgctgtat tgggcctgct
     9181 ggagcaagaa ccggaagcgt tcctgcaaag cggtgcgcag gcagacgaca gcgaagtggc
     9241 tgagattgaa gcgttaattc aacagcgtct ggatgcccgt aaagcgaaag actgggcggc
     9301 ggcagatgcg gcgcgtgacc gtcttaacga gatggggatc gtgctggaag atggcccgca
     9361 agggaccacc tggcgtcgta agtaattgcg ctgttgccgg atgcgagttt tcgcatccgg
     9421 ttatcgcctg cgccaccaca acattcccat aagcaccatc cccggcaacc acacccacat
     9481 caattcagaa ataatcacct gatgcccgta cggcgtggtg taacgagaca atgcaaacgg
     9541 cgcgacttta atcacctgcc agggagcgaa aaagcgttca tctgaccacg gccacagcca
     9601 gccaacgccc ttaccgccag tggttaccga atccagcaag ctgtgcgata gcaacgagac
     9661 ggttaaaaac agccagcagc gaatcagccc agccctgaac catcggcgtc caatcagcac
     9721 acataacagc gggacaacaa acgcaaacac cagcgaatgg gtaaactcgc gatgaccaaa
     9781 aacattgccg taagcaacgc caaatttaaa cgacaatacg tcggcgtcgg gcagcatcgc
     9841 caggatgatt ccggcaaata acagacgcgg agggatgact ttcgaaccca accctaaacc
     9901 aatgcatagg ggaacggcgg cgtgcgtaat aacggttggc atgatggtcg cttcggcaaa
     9961 atgtcgatgc tatcagcatg gatgaacggg gcgtagaggg caaaagtctg aaaagagaac
    10021 cggcctgttg atacaggccg ggaaagggat caggcaacaa cctgtacgct gtgacctgca
    10081 aaactcactg tctgaccggc gacgattttg cagcgtttgc gcgtttcaac cgcaccgtcg
    10141 actttcacct ggccttcggc aatcgcgatt ttcgcctgcg cgccgctttc gctccagcct
    10201 tccagtttca gcaagtcgca cagctcaacg tgcggatgtt tacctaaaga aaatgtcgcc
    10261 atgttactca tcctgtggat catgatattc aacgcacgcc tgtagcgtgt tttcaatcag
    10321 cgtggcaacc gtcatcgggc caacgccgcc cggaacaggc gtaatgtatg aggcgcgttt
    10381 agccgcgtct tcaaacacga cgtcgcccac aactttgcca ttttccagac ggttgatgcc
    10441 gacatcaatc acaattgcgc cttctttgat ccagtcacca ggaataaagc ccggcttgcc
    10501 gacggcaacg atcaacagat cggcattttc tacgtggtga cgcagatttt tagtgaaacg
    10561 gtgagtcacg gtagtggtgc aacctgcgag caacagttcc atgctcattg gacgaccgac
    10621 gatattcgat gcgccaatca ccacggcgtt gaggccgaag gtatcaatgt tgtaacgctc
    10681 aagcagcgtg acgataccgc gcggggtgca gggacgcaga cgcggcgcgc gctggcacag
    10741 acgaccgacg ttataaggat ggaaaccgtc cacgtctttg tccggatgaa tacgttccag
    10801 cactttgacg ttatcaatac ccgccggtaa cggcagttga accagaatgc catcgatggt
    10861 gttgtcggca ttcagcgcat cgataagctc caacagctcc gcttcgctgg tggtttccgg
    10921 gaggtcataa gagcgggaga cgaacccgac ttcttcacaa gccttgcgtt tgcttgcgac
    10981 ataaatttgc gatgcagggt tactacccac cagcacaacg gccagtcctg gtgcccgcag
    11041 tccggctgca atacgcgcct gaactttttg agcaacttca gagcgcacct gctgcgcaat
    11101 cgttttaccg tcaataatct ttgctgccat cagagagagg attccatctg ttacgtaggt
    11161 cgaaggggat gcgcctattt tgtcagaagc ggggcgcgct gtcaggtttc gtttcagatt
    11221 tatcgcgtga agcgacctct tgcgaaggtg aggcgcaccg tcgctgaggt cgctgagatt
    11281 gaaagcttca tttttcgtcc atgatggcgt tgtaaatctg gaactgattt atttccttgt
    11341 ttaaggatta agataattta agaaatacct gacaatataa aaagaatttt cagcctggta
    11401 atttaccgct tcaggtctat atttgtgttg aatatatttt gcgcgaaagt attcatctaa
    11461 cggggctctc tattttttag aatagagtgc atattttcaa ttaagacatt cttagaggat
    11521 aaaaaggaat tttactacta tcagtgtctt aaataaagta atcggttata tacggatgtg
    11581 gagtcgataa atgagattga aggaatatat atgaaattaa gatttatttc gtctgcgctg
    11641 gctgccgcac tattcgccgc tacgggtagt tatgctgccg ttgtagatgg cggtacaatt
    11701 cactttgaag gcgaactggt gaatgctgcc tgttcagtga atactgactc ggcagaccag
    11761 gttgtcacac tcggtcaata tcgtaccgat attttcaatg ctgttggtaa tacctctgca
    11821 ttaattccat tcaccattca gttgaacgac tgcgatcctg ttgttgccgc taatgctgcc
    11881 gttgcatttt ctggtcaggc tgatgcaatc aatgataatt tattggccat tgcatccagt
    11941 accaatacaa caacagcaac gggtgtcggt attgaaatac ttgataatac atccgcaatt
    12001 ctcaaacctg atgggaatag cttctcaacc aaccagaact tgatccccgg gaccaacgtt
    12061 cttcattttt ctgcacgtta taaaggcacc ggtacaagtg catcagcagg gcaagcaaat
    12121 gctgacgcga cttttattat gagatacgaa taatcaaaac cacgttgttt tgaattatat
    12181 atcacgtctt ataacaaagt aatgtaccgg ttgtctgaag cggtatggtg gcaatgtaaa
    12241 tcgaaatcat gttcactttg tatcatgccg ctttattaaa tgaaaaggga atgatgtgtt
    12301 gtaagaaacc aaagcaatca tttctttata tttcttattt ttgccgtcag gaatacacaa
    12361 ggcgtattaa ctatgatgac taaaataaag ttattgatgc tcattatatt ttatttaatc
    12421 atttcggcca gcgcccatgc tgccggaggg atcgcattag gtgccacgcg tattatttat
    12481 cccgctgatg ctaaacagac tgcggtatgg attagaaata gccataccaa tgagcgcttt
    12541 ctggtcaatt cgtggattga aaacagcagc ggtgtaaaag aaaagtcatt catcattaca
    12601 ccgccactgt ttgttagtga acccaaaagc gaaaatacct tgcgtattat ttacaccggt
    12661 ccaccgctgg cagcagatcg tgagtctctg ttctggatga atgttaagac gatcccttcg
    12721 gtagataaaa atgcattgaa cggcaggaat gttttgcaac tggcgatttt atcgcgcatg
    12781 aaattatttc tccgtctaat tcaattacaa gaattacccg cagaagcacc ggacacactc
    12841 aagttttcgc gatccggtaa ctatatcaat gttcataatc cctcaccttt ttatgtcact
    12901 ctggttaact tacaagtggg cagccaaaag ttggggaatg ctatggctgc acccagagtt
    12961 aattcacaaa ttcccttacc ctcaggagtg cagggaaagc tgaaatttca gaccgttaat
    13021 gattatggtt cagtaacccc ggtcagagaa gtgaacttaa actaaccgaa tcatctgaca
    13081 atatcagagc taattatgaa aatacccact actacggata ttccgcagag gtatacctgg
    13141 tgtctggccg gaatttgtta ttcatctctt gccattttac cctccttttt aagctatgcg
    13201 gaaagttatt tcaacccggc atttttatta gagaatggca catccgttgc tgatttatcg
    13261 cgctttgaga gaggtaatca tcaacctgcg ggcgtgtatc gggtagatct ctggcgtaat
    13321 gatgagttca ttggttcaca ggatatcgta tttgaatcga caacagaaaa tacaggtgat
    13381 aaatcaggtg ggttaatgcc ctgttttaac caggtactcc ttgaacgaat tagccttaat
    13441 agcagtgcat ttcccgagtt agcccagcag caaaacaata aatgcatcaa tttactgaaa
    13501 gctgtacctg atgccacaat taactttgat tttgcagcga tgcgcctgaa catcactatt
    13561 cctcagatag cgttgttgag tagcgctcac ggttacattc cgcctgaaga gtgggatgaa
    13621 ggtattcctg ctttactcct gaattataat ttcaccggta acagaggtaa tggtaacgat
    13681 agctattttt ttagtgagct cagcgggatt aatattggcc cgtggcgttt acgcaacaat
    13741 ggttcctgga actattttcg cggaaatgga tatcattcag aacagtggaa taatattggc
    13801 acctgggtac agcgcgccat tattccgctg aaaagtgaac tggtaatggg agacggcaat
    13861 acaggaagtg atattttcga tggtgttgga tttcgtggta tacggcttta ctcttccgat
    13921 aatatgtatc ctgatagcca gcaagggttt gccccaacag tacgtgggat tgcccgtacg
    13981 gcggcccagc taacgattcg gcaaaatggt tttattatct atcaaagcta tgtttccccc
    14041 ggcgcttttg aaattacaga tttgcacccg acatcttcaa atggcgatct ggacgtcacc
    14101 atcgacgagc gcgatggtaa tcagcagaat tacacaattc cgtattcaac agtgccaatt
    14161 ttacaacgcg aagggcgttt caaatttgac ctgacggcgg gcgattttcg tagcggtaat
    14221 agtcagcaat catcgccttt cttttttcag ggtacggcac tcggcggttt accacaggaa
    14281 tttactgcct acggcgggac gcaattatct gccaattaca ccgccttttt attagggctg
    14341 gggcgcaacc tcgggaactg gggcacagtg tcgctggatg tgacccatgc gcgcagtcag
    14401 ttagccgacg acagtcgtca tgagggggat tccattcgct tcctctatgc gaaatcgatg
    14461 aacaccttcg gcaccaattt tcagttaatg ggttaccgct attcgacaca aggtttttat
    14521 acccttgatg atgttgcgta tcgtcgaatg gaggggtacg aatatgatta cgattacgac
    14581 ggtgagcatc gcgatgaacc gataatcgtg aattaccaca atttacgctt tagccgtaaa
    14641 gaccgtttgc agttaaatat ttcacaatca cttaatgact ttggctcgct ttatatctct
    14701 ggtacccatc aaaaatactg gaatacttcg gattcagata cctggtatca ggtggggtat
    14761 accagcagct gggttggcat cagttattca ctctcatttt cgtggaatga atctgtaggg
    14821 atccccgata acgaacgtat tgtcggactt aatgtttcag tgcctttcaa tgttctgacc
    14881 aaacgtcgct acacccggga aaatgcgctc gaccgcgctt atgcctcctt taacgccaac
    14941 cgtaacagca acgggcaaaa tagctggctg gcaggtgtag gtgggacctt actggaaggc
    15001 cacaacctga gttatcacgt aagccagggt gatacctcga ataatgggta tacgggcagc
    15061 gccacggcaa actggcaggc cgcttacggt acgctggggg tcgggtataa ctacgaccgc
    15121 gatcaacatg acgttaactg gcagctgtct ggcggtgtgg tcgggcatga aaatggcata
    15181 acgctgagcc agcctttagg ggataccaat gttttgatta aagcgcctgg cgcaggcggt
    15241 gtacgcattg aaaatcaaac tggcatttta accgactggc gcggctatgc ggtgatgccg
    15301 tatgccacgg tttatcggta taaccgtatc gcgcttgata ccaatacgat ggggaattcc
    15361 atcgatgttg aaaaaaatat tagcagcgtt gtgccgacgc aaggcgcgtt ggttcgtgcc
    15421 aattttgata cccgcatagg cgtgcgggcg ctcattaccg ttacccaggg cagaaaaccg
    15481 gtgccgtttg gatcactggt acgggaaaac agtaccggaa taaccagtat ggtgggtgat
    15541 gacgggcaag tttatttaag tggtgcgcca ttgtctggtg aattactggt tcagtgggga
    15601 gacggcgcga actcacgctg cattgcgcac tatgtattgc cgaagcaaag cttacagcaa
    15661 gccgtcactg ttatttcggc agtttgcaca catcctggct cataaaggaa attatcaata
    15721 agataatctg cagattatta ttggcgatgg catgtttgtg tctggcaaac atatcctggg
    15781 ctactgtttg tgcaaatagt actggcgtag cagaagatga acactatgat ctctcaaata
    15841 tctttaatag caccaataac cagccagggc agattgttgt tttaccggaa aaatccggct
    15901 gggtaggtgt ctcagcaatt tgtccacccg gtacgctggt gaattataca taccgtagtt
    15961 atgtcaccaa ctttattgtt caggaaacta tcgataatta taaatatatg caattacatg
    16021 attatctatt aggtgcgatg agtctggttg atagtgtgat ggatattcag ttccccccgc
    16081 aaaattatat tcggatggga acagatccta acgtttcgca aaaccttcca ttcggggtga
    16141 tggattctcg tttaatattt cgtttaaagg ttattcgtcc ctttattaac atggtggaga
    16201 tccccagaca ggtgatgttt accgtgtatg tgacatcaac gccttacgat ccgttggtta
    16261 cacctgttta taccattagt tttggtggcc gggttgaagt gccgcaaaac tgcgaattaa
    16321 atgccgggca gattgttgaa tttgattttg gtgatatcgg cgcatcgtta tttagtgcgg
    16381 cagggccggg gaatcgacca gctggcgtca tgccgcaaac caagagcatt gcggtcaaat
    16441 gtacgaatgt tgctgcgcag gcttatttaa caatgcgtct ggaagccagt gccgtttctg
    16501 gtcaggcgat ggtgtcggac aatcaggatt taggttttat tgtcgccgat cagaacgata
    16561 cgccgattac gcctaacgat ctcaatagcg ttattccttt ccgtctggat gcagctgcgg
    16621 cagccaatgt cacacttcgc gcctggccta tcagtattac cggtcaaaaa ccgaccgagg
    16681 ggccgtttag cgcgctgggg tatttacgcg tcgattatca atgaggtacg gagaatgaga
    16741 agagtactct ttagctgttt ctgcgggtta ctgtggagtt ccattggatg ggcagctgac
    16801 cctttaggaa cgattaatat caatttgcac ggtaacgttg ttgatttctc ctgtaccgta
    16861 aacacagcgg atattgataa gacggtagat ttaggcagat ggcctacgac acaattactg
    16921 aacgctggcg ataccacggc actcgtccct tttagcctgc ggctggaggg atgtcctccg
    16981 ggttcagttg cgattttatt tacgggaacg ccggcatccg ataccaacct gctggctctg
    17041 gatgatcccg caatggcaca aaccgtcgcc atcgaattac gtaatagcga tcgctcccgg
    17101 ctcgcactgg gggaggcgag cccgactgag gaagtagatg caaatggcaa tgtcacacta
    17161 aacttttttg ccaattatcg agcgttagcc agcggtgttc ggcctggtgt ggcgaaagcg
    17221 gatgcgata
//
LOCUS       DAZKGS010000094        17208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKGS010000094 DAZKGS010000000
VERSION     DAZKGS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..593
                     /locus_tag="ACHOQY_003089"
     CDS             9..593
                     /locus_tag="ACHOQY_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9679142.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            673..1368
                     /locus_tag="ACHOQY_003090"
     CDS             673..1368
                     /locus_tag="ACHOQY_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9679143.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1398..3914
                     /locus_tag="ACHOQY_003091"
     CDS             1398..3914
                     /locus_tag="ACHOQY_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9679144.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3925..5016
                     /locus_tag="ACHOQY_003092"
     CDS             3925..5016
                     /locus_tag="ACHOQY_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9679145.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="ACHOQY_003093"
     CDS             complement(5059..5919)
                     /gene="rsmI"
                     /locus_tag="ACHOQY_003093"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9679146.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHOQY_003094"
     CDS             5983..8019
                     /gene="lpoA"
                     /locus_tag="ACHOQY_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9679147.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7977..8372
                     /locus_tag="ACHOQY_003095"
     CDS             7977..8372
                     /locus_tag="ACHOQY_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9679148.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8392..8982
                     /gene="diaA"
                     /locus_tag="ACHOQY_003096"
     CDS             8392..8982
                     /gene="diaA"
                     /locus_tag="ACHOQY_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9679149.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8992..9567
                     /gene="dolP"
                     /locus_tag="ACHOQY_003097"
     CDS             8992..9567
                     /gene="dolP"
                     /locus_tag="ACHOQY_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9679150.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9681..10721)
                     /locus_tag="ACHOQY_003098"
     CDS             complement(9681..10721)
                     /locus_tag="ACHOQY_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9679151.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10794..11429)
                     /locus_tag="ACHOQY_003099"
     CDS             complement(10794..11429)
                     /locus_tag="ACHOQY_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9679152.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHOQY_003100"
     CDS             11557..12075
                     /gene="yhbO"
                     /locus_tag="ACHOQY_003100"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9679153.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12055..12498)
                     /locus_tag="ACHOQY_003101"
     CDS             complement(12055..12498)
                     /locus_tag="ACHOQY_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9679154.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHOQY_003102"
     CDS             12549..12851
                     /gene="yhbQ"
                     /locus_tag="ACHOQY_003102"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9679155.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12838..13341)
                     /locus_tag="ACHOQY_003103"
     CDS             complement(12838..13341)
                     /locus_tag="ACHOQY_003103"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9679156.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13335..13859)
                     /locus_tag="ACHOQY_003104"
     CDS             complement(13335..13859)
                     /locus_tag="ACHOQY_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9679157.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14068..15063
                     /locus_tag="ACHOQY_003105"
     CDS             14068..15063
                     /locus_tag="ACHOQY_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9679158.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15072..15950
                     /locus_tag="ACHOQY_003106"
     CDS             15072..15950
                     /locus_tag="ACHOQY_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9679159.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16156..17163
                     /locus_tag="ACHOQY_003107"
     CDS             16156..17163
                     /locus_tag="ACHOQY_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP9679160.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLACLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4336 a         4560 c         4354 g         3958 t
ORIGIN      
        1 ggttataaat gaataaagtt acaaaaacag ctattgtggg cttacttgcg cttttcgcag
       61 gtaatgccgc tgcaaccgat ggtgaaatag tttttgatgg cgagatttta aaatccgctt
      121 gtgaaatcaa tgactctgat aagaaaattg aagttgctct tggtcactat aatgccgaac
      181 agtttcgttc tgttggcgat cgtagcccaa aaattccatt cactattcct ttagttaact
      241 gcccagtgac tgggtgggag cacgataacg gcaacgttga agcgtctttc cgtttatggc
      301 tggaaacgcg ggataatggc accgtcccta acttccctaa tctggcaaaa gtgggctctt
      361 ttgctggcac agcggcaaca ggtgtgggta tccgtattga cgatgcggaa agcggaaacc
      421 ttatgccact gaatgctatg gggaatgata acacggttta tcagatccca gcggactcca
      481 atggtattgt caatgttgac ctcatcgctt actacgtatc aaccgttgaa gcatcagaaa
      541 tcaccccagg ggaagcagac gctgtcgtta acgtaacgct ggattaccgt taatacgtta
      601 cggcgttatc tgacctttca gataacgccc ttttccttcc tctttctcgt tgtatcaggt
      661 tgaaaaataa ctatgtcaaa acgaacattc gcggtgataa taaccttgtt gtgtagcttc
      721 tgtatcggcc aggcgcttgc agggggtatc gttttacagc gaacgcgagt gatctatgat
      781 gccagccgca aagaggctgc gttacctgtc gcaaacaaag gcgcagaaac gccttattta
      841 ctgcaatcat gggtagataa tatagatggt acaagccgtg ccccatttat tataacccca
      901 ccgctatttc gtcttgaagc cggcgatgac tcatcactgc gaattattaa aacagctgat
      961 aacctgcctg aaaataaaga gtcgctgttc tacattaatg ttcgtgccat tccagcaaag
     1021 aaaaaatcag atgatgttaa tgctaacgag ttgacgctgg tatttaaaac acggatcaaa
     1081 atgttttatc gccccgcaca cctgaaggga cgggtaaacg atgcgtggac atcactggaa
     1141 tttaaacgta gtgaccattc actcaatata tataacccaa ctgaatatta cgtcgtattt
     1201 gccggactgg cagtcgataa aaccgatctc acaagcaaaa ttgaatatat cgcgcccgga
     1261 gaacataaac agttaccact tcctgcatct ggcggaaaga acgtgaaatg ggctgcaatc
     1321 aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca aatataaaaa
     1381 acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc acaccgaaac
     1441 agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc ggcatgcttt
     1501 gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa actacaaaaa
     1561 gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa ctaccaggaa
     1621 cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa attacattta
     1681 ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta cgtgagctgg
     1741 gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc gtgatcaact
     1801 cgcttgaaca aatcattccc ggtacagctg ctgaatttga tttcaatcat cagcgactta
     1861 atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc tccccttctc
     1921 gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt tctgataacc
     1981 gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat ggtgctaatt
     2041 ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag gcgtcaagct
     2101 ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct cagttgcttc
     2161 tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact ggcgtgcaac
     2221 tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca acggtacgcg
     2281 gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg atctatcaaa
     2341 gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc aacagcggcg
     2401 atttagaagt cacgattgaa gaaagtgacg gtacgcaacg tcgctttatc cagccttatt
     2461 cttcattacc catgatgcag cgacctgggc atctaaaata tagcgcgacc gctggacgct
     2521 atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc acggcaatat
     2581 atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa gattattatg
     2641 cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg atggatatca
     2701 acagagctga cacccaattc gataaccggc actcttttca tggctatcaa tggcgtacgc
     2761 agtacatcaa agatatcccg gaaaccaaca ccaatatcgc tgttagctac tatcgctata
     2821 ccaacgatgg ctattttagt tttgatgaag ccaatacccg taattgggac tataacagtc
     2881 gccaaaaaag tgaaattcaa ttcaacatca gccagacaat atttgatggg gtaagtctgt
     2941 atgcctccgg ttcgcagcaa gactattggg gcaataacga taaaaacagg aatatctcag
     3001 ttggggtttc cggtcagcaa tggggaattg gttacagcct gaattatcaa tacagtcgct
     3061 acacggatca aaataatgac cgcgcactct ctttgaatct cagtattccg ttagaacgct
     3121 ggttaccgcg tagccgggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
     3181 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
     3241 aaaatctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
     3301 atggcacctt cagtgccgga tacagctacg gtaatgacag cagccaatac aattacggcg
     3361 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
     3421 cttttgcgct tatcgatgct aatggggctt ctggcgtgag gatacaaaac tatccgggga
     3481 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttac caggaaaacc
     3541 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgagcaa acaacacagt
     3601 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
     3661 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
     3721 gcaacgatga gacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
     3781 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
     3841 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacgg
     3901 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
     3961 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagctgacag cactagcggt
     4021 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
     4081 ggacgggagt ttacctctgc cacgctaagt agcactaact ggcaatacgc ctgtacctgc
     4141 tctgcgggta aggcagttaa acttgtctat atggtcagcc ctgtacttac caccactgga
     4201 catcaagcag gatattacaa actcaatgat agtctggata ttaaaaccac attacaggcg
     4261 aatgacattc caggactcgt gactgaccag accgtttctg ttaacacccg attcacacag
     4321 ataaaaagca acaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     4381 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     4441 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcatgg
     4501 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     4561 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaggtt
     4621 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     4681 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     4741 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccaga
     4801 cgaagaagtt cagacaatgc gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     4861 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     4921 atcaacatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     4981 cagggcaccg ccaccattac cgtcatcgtg cggtaaaaga agacgttgcg ttttgattaa
     5041 cgcaacgctt cggcactctt acccctgctg ctccagcgca tacttataca gcgcattttt
     5101 cttcacgccg tgaatttctg ccgccagcgc cgccgctttt ttcaacggca gttctgcctg
     5161 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     5221 ttcgataatc agcaccattt cgcctttgcg acggttttca tcttctttta cccacgccag
     5281 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     5341 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     5401 gtgggtggat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     5461 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagagggtaa
     5521 acccgcggcg cttaacgcag tgatagcagc acacggcccg ggtagcggca ccacgcaaat
     5581 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     5641 ggcatcggaa accagcgcaa tgttctgccc ttcttgcagc ttcgccagca gcgtttcggc
     5701 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     5761 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     5821 ctctaacgca cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     5881 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     5941 ttaatattga gcattgcgta aaaaaatatc actggataca ttatggtacc ctcaacattt
     6001 tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg cagccctgat tttcgccggt
     6061 tgtggcaccc atactcccga tcagtccact gcttatatgc agggcacggc gcaggctgat
     6121 tctgcctttt atcttcagca gatgcagcaa agctctgatg ataccaggat caactggcaa
     6181 ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg ggcaggcggt tgagttgttt
     6241 aaccaactac cgcaagaact gaacgattct cagcgtcgcg agaaaacact gctggcggta
     6301 gagattaaac tggcgcagaa agattttgct ggcgcgcaaa acttgctggc gaaaatcaca
     6361 cctgccgatt tagaacaaaa ccagcaagcg cgttactggc aggcaaaaat cgatgccagc
     6421 caggggcgtc cttccattga tttactgcgc gcgttaattg ctcaggaacc gctgctcggc
     6481 gcgaaagaaa aacagcagaa tattgatgcc acctggcagg cgctctcctc catgactcag
     6541 gaacaggcga atacgctggt gatcaacgcc gacgaaaata ttctgcaagg ctggctggat
     6601 ctgcagcgcg tctggtttga taaccgtaac gatcccgaca tgatgaaagc cgggatcgcc
     6661 gactggcaaa aacgttatcc gaacaatccg ggcgcgaaaa tgctgccaac gcagttggtt
     6721 aacgtaaaag cgtttaaacc agcctcgacc aacaaaatcg ccctgctgtt gccgctgaat
     6781 ggccaggcag cggtatttgg tcgcactatt cagcaaggct ttgaagcggc gaaaaatatc
     6841 ggcactcagc cagtggcggc tcaggtagct gccgcacctg ccgcagacgt agctgaacaa
     6901 cctcagccgc aaaccgtgga tggcgttgcc agcccggcac aagcctcggt tagcgatctg
     6961 accggtgaac agcctgcggc acagccagcg cctgtaagca ccccggcagc aaccaccaca
     7021 gcggtaagcg cgccagcgaa tccatccgca gaactgaaaa tctacgatac ctcatcacaa
     7081 ccacttagcc agatcttaag ccaggttcag caggatggcg cgagtattgt ggttggtccg
     7141 ttgctgaaaa ataacgttga agagttgctt aagagcaaca ccccgctgaa cgtgctggca
     7201 ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt gttacttcgc gctttcaccg
     7261 gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc agggtaaaca agcgccgctg
     7321 gtgctgatcc cacgcagttc attgggcgat cgcgtagcca atgcgtttgc gcaggagtgg
     7381 cagaaactgg gcggcggcac cgttctgcaa caaaaatttg gttccaccag cgaattacgc
     7441 gcgggtgtta acggcggttc tggtatcgct ttaacgggta ccccgattac tcccagagcg
     7501 acaaccgact ccggcatgac gaccaacaat ccaacgctgc aaaccacgcc aaccgatgac
     7561 cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca ttgtggcaac gccgggtgaa
     7621 atcgctttta tcaaaccgat gatcgccatg cgtaacggta gccagagcgg tgcaacgctg
     7681 tacgccagct cccgcagtgc gcaagggacc gctggcccgg atttccgact ggagatggaa
     7741 ggcttgcagt acagcgaaat cccgatgctg gcaggcggta atctaccgtt aatgcagcag
     7801 gcactcagcg cggtgaataa cgattattca ctggctcgca tgtatgcgat gggcgtcgat
     7861 gcctggtcgc tggcaaatca tttctcacaa atgcgccagg ttcagggttt tgaaatcaac
     7921 ggtaataccg gaagcctgac ggcaaacccg gattgcgtga ttaacaggaa gttatcatgg
     7981 ctacagtacc aacaaggtca ggtagtcccc gccagttaac caccaaacag accggcgatg
     8041 cgtgggaagt acaagcgcgt cgctggctgg aaggcaaagg actgcgattt gtcgccgcta
     8101 acgtgaacga gcgtggcggc gagatcgatc tgataatgcg tgaaggccgg accaccgttt
     8161 ttgtcgaggt acgctatcgc cgctctgcgc tttatggcgg cgcggcagcc agtgtgaccc
     8221 gcagcaaaca acacaaatta ttacagactg cccgcttgtg gctcgcgcgt cataatggga
     8281 gttttgatac tgtggattgc cggttcgatg tggtagcctt caccgggaat gaggttgagt
     8341 ggattaagga tgcctttaat gaccactcat aattaaggtt taaggattag cgtgcaagaa
     8401 agaattaaag cttgcttcac tgaaagcatt caaactcaaa ttgcggcggc agaggcgctt
     8461 ccggatgcca tctcccgtgc agccatgacg ctggttcagt ctctgctcaa tggcaacaaa
     8521 atcctctgtt gtggtaatgg aacttccgct gccaatgcac agcattttgc tgccagcatg
     8581 atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca ttgcactaaa tactgataat
     8641 gttgtcttaa cggcgattgc caacgatcgc ttacatgatg aagtgtatgc aaaacaggtg
     8701 cgggcgctgg gtcatgcggg agatgtattg ttagccattt ccacccgtgg caacagccgc
     8761 gatattgtaa aagcggtaga agctgccgtt acgcgtgata tgactattgt ggcattgacc
     8821 ggctatgacg gcggcgaact tgcaggtttg ttagggccac aagatgtgga gatccgcatt
     8881 ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc tgacggtaaa ttgcctgtgc
     8941 gatctgatcg ataacacgct tttccctcac caggatgttt aaggagaata catgaaggca
     9001 ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc aaggttgtgt tgccgctgcc
     9061 gtagtgggta ccgctgctgt gggtaccaaa gccgcaactg acccacgcag tgtcggcacc
     9121 caggtggacg atggtaccct ggaagtgcgc gtgaacagcg cattgtcgaa agacgaacag
     9181 attaagaaag aagcccgtat taatgtcacg gcgtatcagg gcaaagtgct gctggttggg
     9241 cagtcaccaa acgctgaact ttcggctcgc gccaaacaga ttgctatggg cgtagacggt
     9301 gccaacgaag tgtataacga gattcgtcag ggccagccga ttggtctggg cgaagcgtct
     9361 aacgatacgt ggatcaccac caaagtgcgt tcgcagctct taaccagcga cctggtgaaa
     9421 tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt tcctgatggg gctggtgact
     9481 gaacgtgaag cgaaagcggc ggcagatatt gccagccggg tgagcggcgt aaagcgggta
     9541 accacagcgt ttacgtttat taaatagcag ccccttgtaa tgcctgatgc gacgcttgcc
     9601 gcgtcttatc aggcctacaa cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat
     9661 ccgacatgtt ttccctcaaa tcagaacaac agcgccagcc cgccgacaat cacaccagac
     9721 actgccacca tcgcccctgt cagccataag gctttcgctg ggaacgcttt gcgcagcata
     9781 atcagtgacg gcaaactcac cgccgggagc gtcatcaaca atgccagcgc cggagcagtt
     9841 cccatacctg ccagcatcat cgtttgtaca atcggaattt ctgctgccgt gggaatgaca
     9901 aacaagcatc ctgctaccgc catcgccatc acccacatca ggctgttatc gacagcacca
     9961 tcggcatggg ggaataacca gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa
    10021 accgggatcg tactccagaa aagcgtccat agcgccctgc cccagcggct aaaaaaaccg
    10081 ccctgtgctt ccggtatgtc aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc
    10141 catttttgca ccagcgtcgc aatcagcaac accatcacca gcccggccac cagacgaatc
    10201 gccgcaaaac cccagccgag gacaaacccc ataaacacca gcgtcgccgg gtttaacacc
    10261 ggattgccca tccagaatgc cagcgcaccg cccatcgaca cctgttggcg acgcattccc
    10321 gccgcgaccg gagccgcaca gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc
    10381 agcagcgtgc cgcgaaagcg cgattgccca agcgtacgca acaaccagtc acgcgggatc
    10441 agcacctgaa tcaacgaacc gaggatcacc cccagcactg ccgctttcca taccgcgagg
    10501 aaatagagca tcgcgtaatc caacgccgcc tgccacggat tagcatccgc ctgcgcaagg
    10561 atagatttac cgatactgtg ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc
    10621 catttcacat accagagacc ggcaatgaca acgagaaaga aaagcgcggg tttccaccac
    10681 tgaatgggcg ttgccgcctg agatgaagac tgaccagtca tagcattccc caggaataag
    10741 ttatgataat tgagcgcgtg aatattacgc tcactatcaa ttcttgggaa taattattca
    10801 gctcttttgc gtaattctga agagcttaaa atcgtcacac cttcatgccc gggccgcatc
    10861 gcctctgcca gcatgacccg cgccacgtct ctcgcatcaa tggatttcca gttaccgggt
    10921 aataaacgga acagcggcgc aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc
    10981 agtaacatcg acgggcgagc aatggtcaat ttcggccagt tctgggcgat taatgcttct
    11041 tccatctccc ctttgacgcg gttatagaaa aacggcgagt gggcattggc acccatagca
    11101 ctgaccacca gcatatgctg cgcgcccagt cgccgcccgg ttaatgcggt atccactacc
    11161 agcgtgtaat cggcatgaat aaacgcctct ttgctccccg cttctcgccg cgtggtgccg
    11221 agacaacaaa acacaatgtc gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc
    11281 ggatcatggg gattaaacac cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct
    11341 atggcgttaa ctttcggttc gttaatcaac atccgcagca ggtgaccgcc caccagcccc
    11401 gttgcgcctg taatcagtac ctgactcatc ttcgctcctt tacagaattg tctgccttgc
    11461 gctccacggc tcatgcacca ggcttaatag accatgaggt aattatcccc gattgtggga
    11521 aattcgcctc atccaatgca acaacgcgga ggaagcatga gtaagaaaat tgccgtttta
    11581 atcactgatg aatttgaaga ttcagaattt acttcacccg cagacgagtt ccgtaaagcc
    11641 ggacacgaag tgattaccat tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga
    11701 gaagccagcg tgaccatcga taaatccatc gatgaagtga cgccagcgga gtttgatgcc
    11761 ctgctgctac cgggcggcca ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc
    11821 tttacccgtg attttgtgaa tagcggcaaa cctgttttcg ccatctgtca cggtccgcag
    11881 ctgctgatca gcgccgatgt gattcggggg cgcaaactga ccgcggttaa accgatcatt
    11941 attgatgtta aaaacgcggg cgcggaattt tacgatcagg aagtcgtggt cgataaagat
    12001 cagctggtta ccagccggac accggacgat ctgccagcgt ttaaccgcga ggcgttacgc
    12061 ctgctcggtg cctgagccgc gtagccaaat cattttttta ccaaagccca gcgtgttgtc
    12121 ggtgaatttg atttcatcga gccggatttc ccacaccggg gccgacagca ttctggcaac
    12181 cggaaagcga cgattgtacg ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg
    12241 gatctcacct ttaaactgca cgccgcgaat taacgctacc gttttcggct gaccgtttac
    12301 cgttccggca accgcagcct gcggcccact catctgcgcg tggcgcgttt tttcttccgt
    12361 caaaatgtag aaggcgactt tctgcgcatc aaacagataa aaagcattag cgcaccagag
    12421 ttccccttcc tgctgcacac accaggtgac aacatgttgt tttgccaacc aacggctgat
    12481 ggtgatgagt gtttccattg ctgttctctt ttatactgtg ggcctgacct taacatgcga
    12541 cgcagacgat gacaccctgg tttctttact tgatccgtac cgccgacaat aagctttata
    12601 ccgggatcac cacggatgtc gaacgccgct atcagcagca ccaaagcggc aaaggggcga
    12661 aagcactgcg cgggaaagga gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt
    12721 cgctggcgtt acgggcggaa tatcgcgtta agcaactgac caaacggcaa aaagagcgtc
    12781 tggtagcgga aggcgcaggg tttgcagagc tgttaagcag tctgcaaacc ccggagatta
    12841 aaagcgattg aaatgctcgt gatactcaac cagaccagtt acgccattca acgcgtcatc
    12901 cgctaaacga tgtacctgga aggcgctttc agtgcctggc cagcggcaac gcaggtcgtg
    12961 atgtgccgcc agttcaaagc caaaacggct gtacagcgcc ggatcgccca gcgtcaccac
    13021 tgcggcatag ccgaactcat taagcgaatc gagtccttca tagaccagtt ggcgtgccag
    13081 cccctgtcca cggtattttt catcaaccgc caacggtgcc atgccgaccc attgcaggtc
    13141 ttcgccctgc acatcaaccg gactaaatgc cacatagcca atgacctgac cttcgtcatc
    13201 tgtcgccacc agccccagcg tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc
    13261 ttccgcatca ctttcgaatg agcgacgtag cagggcatca atacccggcg catcaatggg
    13321 aatttctact cgaattagca tggttcacct accgatgtct gtttggtttc aggcgcggtt
    13381 ttcattcccg cctcaacaaa atccgccagt tgcagcagca tcatgcgtaa ggctttcggc
    13441 atttgctcca gttcaatggc gtccatcagg tttttcacat acagccccag ctccgtatcg
    13501 ccttcaatca ccagccgacg ctggaagaag agcgtatccg gatcttgttt acgcgccgca
    13561 atcatcagca gatcgctggc gtcggcacta aaactcacat cagcttgcgc gttctggctg
    13621 accaccagtt tgccattcac caccgaggta aaccattgca ggtcaatatc acgcacatga
    13681 atacttaacc agcggccttc aagaaactcc agctcgccat catccagcgc ctgacggaat
    13741 tgccagctta agacctgctc aagaacctgg cgttttagcg caaatggcgt cagttttacc
    13801 ggtacactca acagagatgg ccccaaatgc acaatacggg aacgcagttt atccaacacg
    13861 agttttactc cctgtttcaa caatcatcct attttgccat atcagaaaaa taacatagcg
    13921 gtataaatca acaattccat atgaaattgc tgctaccacc aatacaactt taactgcctt
    13981 aaatcaaaaa ttgtcgcagc aaggttaact aaaatcccag ttcgttaaca tttttgcgtt
    14041 ttgatagcgc aaccttcagg aaaaattatg gagctgctct gccctgccgg aaatctcccg
    14101 gcgcttaagg cggccatcga aaacggcgca gatgctgttt atatcgggct aaaagatgat
    14161 accaatgccc gtcacttcgc cggccttaac tttaccgaga aaaaattgca ggaagcggtg
    14221 agttttgtcc atcaacatcg ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg
    14281 gacggttacg cccgttggca gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg
    14341 ctgatcctcg ccgacctcgc catgctagag tacgccgccg agcgttaccc gcatattgag
    14401 cgtcatgtgt cagtgcaggc ttcggcgacc aatgaagagg cgattaactt ttatcatcgc
    14461 cattttgacg ttgctcgcgt ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa
    14521 ctggcacggg tcacacctgt accactggaa gtctttgctt tcggcagcct gtgcattatg
    14581 tcggaaggtc gttgctatct gtcgtcgtat ctgacgggtg agtcgcccaa caccgtaggc
    14641 gcgtgttctc cggcccgttt cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc
    14701 ctgaacgaag tgctgatcga ccgttatcag gacggcgaaa acgcaggtta tccgacgcta
    14761 tgtaaagggc gttatctggt ggacggcgag cgctatcacg cgctggaaga accaaccagt
    14821 ctcaataccc tggaactgct gccggagtta atggcggcga atattgcttc ggtgaaaatt
    14881 gaaggccgcc agcgtagccc ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct
    14941 atcgaccgtt gtaaggccga tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg
    15001 ctcgggtcga tgtccgaagg cacgcagacc acccttggcg cgtatcaccg taaatggcag
    15061 tgagaagagc aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc
    15121 tggaagattt ttatcagcag gccgccacca gcagcgccga cgtgatttat cttggtgaag
    15181 cggtatgcag caagcgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
    15241 tcgccggaag tggcaagcag attgtgctct ccacgctggc gctggtgcag gcatcctctg
    15301 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
    15361 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga
    15421 actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
    15481 tgccggtgga gctttcccgc gactggctgg tgaatctgct taatcagtgc gatgagctgg
    15541 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctaccg ctggcctact
    15601 ccgcccgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
    15661 gcattaagta tccgaacggg cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac
    15721 tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctggcatcca
    15781 tgcaggggct ggttgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcgatgc
    15841 tcgacgcctt ccgcgctaat gaaaatggcg ctgcgccact accgttgacg gcaaacagcg
    15901 actgtaacgg ctactggcgg cggctggccg ggctggagct gcaagcctaa gtaaatatgc
    15961 tcgtcatact tcaagttgca tgtgctgcat ctgcattcgt tcaccccagt cacttactta
    16021 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagca
    16081 cagctcactt tgttaacaac tttaactact ctttaatgca gtattaaaga ttaatcggta
    16141 acaaagtgag ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc
    16201 attcccgaag gttcttcagc gcgagaagca ttctcccact ctctcgatct cgcctgtctg
    16261 gctgaaaagc gcggctatca tcgctactgg ctggcagaac accacaatat gactggcatt
    16321 gccagtgctg ccacgtcggt attgattggc tatctggcgg cgaataccac cacgctgcat
    16381 ctggggtctg gcggcgtgat gttgcctaac cactcaccgt tggtcattgc cgaacagttc
    16441 ggcacgctca atacactcta tccggggcga atcgatttgg ggctgggtcg tgcgccaggt
    16501 agtgaccaac ggacaatgat ggcgctgcgt cgccatatga gcggcgatat tgataatttc
    16561 ccccgcgatg tcgcggagct ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg
    16621 cgcccggtac caggctatgg cgagaaaatc cccgtgtggt tgttaggctc cagcctttac
    16681 agcgcgcaac tggcggcgca gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg
    16741 gatatgttgt tccaggcgct gcatctttat cgcagcaact tcaaaccgtc ggcacgactg
    16801 gaaaaaccat acgcgatggt gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct
    16861 gagtttctgt ttacctcaat gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg
    16921 caactgccgc caccgattca aaatatggat cagttctggt cgccgtccga acagtatggt
    16981 gtgcagcagg cgctgagtat gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg
    17041 cagtcgatcc tgcgcgaaac cgacgccgat gagattatgg tcaacgggca gattttcgac
    17101 caccaggcgc ggctgcattc gtttgagctg gcgatggatg ttaaggaaga gttgttggga
    17161 tagtgtgtct taacgcggga agccttatcc gagctggcaa cgctgtcc
//
LOCUS       DAZKGS010000095        17066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKGS010000095 DAZKGS010000000
VERSION     DAZKGS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..181
                     /gene="ybiJ"
                     /locus_tag="ACHOQY_003108"
     CDS             <1..181
                     /gene="ybiJ"
                     /locus_tag="ACHOQY_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9679161.1"
                     /translation="ASQAQNMNKIGVVSADGASTLDALEAKLAEKAAAAGASGYSITS
                     ATNNNKLSGTAVIYK"
     gene            complement(413..895)
                     /gene="ybiA"
                     /locus_tag="ACHOQY_003109"
     CDS             complement(413..895)
                     /gene="ybiA"
                     /locus_tag="ACHOQY_003109"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFP9679162.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            1015..3165
                     /gene="dinG"
                     /locus_tag="ACHOQY_003110"
     CDS             1015..3165
                     /gene="dinG"
                     /locus_tag="ACHOQY_003110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9679163.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            3193..4155
                     /gene="ybiB"
                     /locus_tag="ACHOQY_003111"
     CDS             3193..4155
                     /gene="ybiB"
                     /locus_tag="ACHOQY_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9679164.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4296..5381
                     /gene="hcxB"
                     /locus_tag="ACHOQY_003112"
     CDS             4296..5381
                     /gene="hcxB"
                     /locus_tag="ACHOQY_003112"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9679165.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5448..5507
                     /gene="ybiE"
                     /locus_tag="ACHOQY_003113"
     CDS             5448..5507
                     /gene="ybiE"
                     /locus_tag="ACHOQY_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP9679166.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5612..6976)
                     /gene="rhlE"
                     /locus_tag="ACHOQY_003114"
     CDS             complement(5612..6976)
                     /gene="rhlE"
                     /locus_tag="ACHOQY_003114"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9679167.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            7205..7876
                     /gene="cecR"
                     /locus_tag="ACHOQY_003115"
     CDS             7205..7876
                     /gene="cecR"
                     /locus_tag="ACHOQY_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9679168.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7879..8874
                     /gene="hlyD"
                     /locus_tag="ACHOQY_003116"
     CDS             7879..8874
                     /gene="hlyD"
                     /locus_tag="ACHOQY_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9679169.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            8867..10603
                     /gene="ybhF"
                     /locus_tag="ACHOQY_003117"
     CDS             8867..10603
                     /gene="ybhF"
                     /locus_tag="ACHOQY_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9679170.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10596..11729
                     /gene="ybhS"
                     /locus_tag="ACHOQY_003118"
     CDS             10596..11729
                     /gene="ybhS"
                     /locus_tag="ACHOQY_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9679171.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11740..12846
                     /gene="ybhR"
                     /locus_tag="ACHOQY_003119"
     CDS             11740..12846
                     /gene="ybhR"
                     /locus_tag="ACHOQY_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9679172.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12808..13218)
                     /gene="ybhQ"
                     /locus_tag="ACHOQY_003120"
     CDS             complement(12808..13218)
                     /gene="ybhQ"
                     /locus_tag="ACHOQY_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9679173.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13351..14112
                     /gene="ybhP"
                     /locus_tag="ACHOQY_003121"
     CDS             13351..14112
                     /gene="ybhP"
                     /locus_tag="ACHOQY_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9679174.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLRETHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            14109..15350
                     /gene="clsB"
                     /locus_tag="ACHOQY_003122"
     CDS             14109..15350
                     /gene="clsB"
                     /locus_tag="ACHOQY_003122"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9679175.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15350..16306
                     /gene="ybhN"
                     /locus_tag="ACHOQY_003123"
     CDS             15350..16306
                     /gene="ybhN"
                     /locus_tag="ACHOQY_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9679176.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16342..17055)
                     /gene="ybhM"
                     /locus_tag="ACHOQY_003124"
     CDS             complement(16342..17055)
                     /gene="ybhM"
                     /locus_tag="ACHOQY_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9679177.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         3978 a         4456 c         4677 g         3955 t
ORIGIN      
        1 ggcatcccag gcacagaaca tgaataaaat cggcgtggtt tctgccgatg gcgcatccac
       61 cctcgatgcc ctggaagcga aactggctga gaaagccgcg gcagccggtg ccagtggata
      121 cagcatcact tccgccacca acaacaataa attaagcggt actgcggtaa tttataagta
      181 attcgtagga cggataaggc gtttacgccg catccggcaa cagatgcctg atgcgacgct
      241 accgcgtctt atcagtatct acataatctc tgcaatctat tgaatttgca tgtttttgta
      301 ggccggataa ggcgtttacg ccgcatccgg catttcacaa caagcacttg tcagcaattt
      361 gagaacacgg gaaaaaattt atctgtttta ccgcccataa gccaccgaga cgttacttct
      421 ctatagccaa ttgttcgcgc aactccatta aaaggtagcc cagtcgattc ttgcccttac
      481 cattaccacc gtctccccag taagcatcgt tttccgtatg ctcaaccagt tttgcgggcg
      541 cggttgccag caagagcgca cgcagttctg gatgctgttc gaatttagcg cgaagtgctt
      601 ttcgcatcac ctgttctttg accgactccc agtttttacg cagaggctta gaacgatcgc
      661 gccccatgcg tgcggcgacc ataggggaag aaacccgacg gatctcttcg cggtattttt
      721 catcaaggaa tttttgtgcc tgaaaatagt gttctgaggt gggccaggtt ttcccgtcaa
      781 ccttgatggg ccaggcggca aagttagaaa aatcaccgta gtcatcgctg gtgctgtaga
      841 aatttatgat ggtgtcttgc atgacgtgtt ggatcctttg tgctcgaacg ggcattaaac
      901 cgcattatgt tggtggttat tgcgagccgc tttccagaaa cagaaaaacc attacccctg
      961 aaaaccgaaa aatgccacaa tattggctgt ttatacagta tttcaggttt tctcatggca
     1021 ttaaccgccg cgcttaaagc gcaaattgcc gcctggtata aggcgcttca ggaacagatc
     1081 cccgacttta ttccccgtgc gccgcagcgg cagatgattg cggacgtcgc caaaacgctg
     1141 gccggagaag aagggcgaca tctggcgatt gaagccccca ccggcgttgg gaaaacgctc
     1201 tcctatttga tccccggcat cgccattgcc cgcgaagagc aaaaaacgct ggtggtgagt
     1261 accgccaacg tggcattgca ggatcagatt tacagcaaag atttaccgct gctgaaaaag
     1321 atcattcccg atcttaaatt cactgccgct tttgggcgtg ggcgctacgt ttgcccgcgt
     1381 aatctgacgg cgctcgccag cactgagccc acgcaacagg atctgctggc gtttcttgac
     1441 gacgaactga cgccaaataa tcaggaagag caaaaacgtt gtgcgaagct gaagggcgat
     1501 ctcgacactt ataaatggga tggtctgcgc gatcatactg atatcgctat agatgacgat
     1561 ctctggcgtc gtttaagtac cgacaaagcc agctgcctca accgcaactg ttactactat
     1621 cgtgaatgcc cgttttttgt tgctcgtcgg gagattcagg aagcggaagt ggtggtggca
     1681 aaccatgcgc tggtgatggc ggcgatggaa agcgaagccg tactacctga cccgaaaaat
     1741 ctgctgctgg tgctggacga aggtcatcac ctgccagatg tggcccggga tgcgctggag
     1801 atgagtgccg aaatcaccgc gccctggtat cgactacagc tggacttgtt cacgaaactg
     1861 gtcgctacct gcatggagca gtttcgcccg aagaccatcc caccgctggc gatccctgaa
     1921 cgtttgaatg cgcattgtga agagttgtat gagcttatcg cctcgttaaa caacattctc
     1981 aatctctaca tgcctgccgg gcaggaggca gagcaccgtt ttgcgatggg cgaactgcct
     2041 gatgaagtgc tggagatctg ccagcggctg gcaaaactca ccgagatgct gcgtggtctg
     2101 gcggagttat ttcttaacga tttaagtgag aaaaccggca gccacgatat tgtgcgtctg
     2161 catcggttga ttttgcagat gaaccgcgcg ttggggatgt tcgaggcgca aagcaaactc
     2221 tggcggctgg cgtctctggc gcaatcttcc ggtgcgccgg tgaccaaatg ggcgacgcgg
     2281 gaagagcgcg aagggcagct gcatctctgg tttcactgcg tgggaatacg tgtcagcgat
     2341 cagctggaaa ggctgctgtg gcgcagtatt ccgcacatta ttgtcacctc cgcaaccttg
     2401 cgttcgctga acagtttttc gcgtttgcag gagatgagtg gtctgaaaga gaaagcgggc
     2461 gaccgttttg tggcgctgga ttcccccttt aaccactgcg aacagggcaa aattgttatt
     2521 ccccggatgc gctttgagcc ttccatcgac aacgaagagc agcatattgc cgaaatggcg
     2581 gcctttttcc gtaagcaggt ggagagcaaa aaacatctcg gtatgttggt actgtttgcc
     2641 agcggacggg cgatgcagcg ctttctcgac tatgtgacgg atttacgcct gatgctgctg
     2701 gtgcagggcg atcagccgcg ttatcgctta gttgaactgc accgcaaacg cgttgccaac
     2761 ggtgagcgta gtgtgctggt gggcttacag tcatttgccg aagggcttga tttgaaaggt
     2821 gatctgctca gccaggtgca tatccacaaa atcgcttttc cgcccatcga cagcccggtg
     2881 gtgatcaccg aaggggaatg gctgaaaagc ctcaaccgct atccgtttga ggtgcaaagc
     2941 ctgccgagcg cctcgtttaa cctgattcag caggttgggc gactgattcg aagccacggt
     3001 tgctggggcg aagtggttat ctacgacaaa cgcttgctga ccaaaaatta tggcaagcga
     3061 ctactggatg cattaccggt atttccgata gagcaaccgg aagtccctga aggtatagtt
     3121 aaaaagaaag aaaaaacgaa atccccacgc cgtcggcggc gttaatgatg tgagtcaggt
     3181 aaggagtcgt aaatggacta tcgcaaaatc attaaagaga tcgggcgcgg gaaaaaccac
     3241 gcgcgcgatt tagaccggga tactgcgcgc ggtctgtatg cccatatgct caatggtgaa
     3301 gtccctgacc ttgagttggg cggcgtattg attgcgctgc gtatcaaagg ggaaggggaa
     3361 gcagagatgc tcggctttta cgaagccatg caaaatcaca ccatcaagct gacgccgcct
     3421 gcaggcaagc cgatgccgat tgtcatcccc agttacaacg gcgcgcgtaa acaggccaac
     3481 ctgacgccgt tgttggcgat tctcctgcac aaactcggtt ttcctgtggt ggttcacggg
     3541 gttagcgaag atccaacccg cgtgctgact gaaaccattt ttgaattgat gggtattacg
     3601 ccaacgttgc acggcggaca ggcgcaggcg aagctcgacg aacatcaacc ggtgtttatg
     3661 ccagtcggcg cgttttgccc gccgctggaa aaacaactgg cgatgcgctg gcggatgggc
     3721 gtgcgtaaca gtgcacatac cctggcgaaa ctggcaacgc catttgccga aggtgaggcg
     3781 ctgcgtcttt ccagcgtttc gcatccggaa tacattggac gcgtcgcgaa gttctttagc
     3841 gatatcggcg ggcgggcgct actaatgcat ggtactgaag gtgaagtgta tgctaatccg
     3901 cagcgctgcc cgcagatcaa tctcattgac cgtgaaggga tgcgggtgct gtatgaaaaa
     3961 caggacactg ctggtagcga gttactgcca caagcaaaag atccggaaac cacggcgcag
     4021 tggattgagc gttgccttgc tggcagcgaa ccgattcccg aatcgctgaa aatccagatg
     4081 gcttgctgcc tggtggctac gggtgaagcg gcaactatca gcgacggcct ggcgcgcgtt
     4141 aatcaggcat tttaattctt ttctcagccg gatgagccat gtcatccggc ttttacccca
     4201 ccgcaatatg aaattcctgc atctttattg accttcccac gcccggcgtg cagcataaaa
     4261 atacaacaaa cacataacat aaacaggagt taaccatgga aagtggtcat cgctttgatg
     4321 ctcagacgct gcacagtttt attcaggctg tatttcgtca gatgggtagc gaggaacaag
     4381 aagcgaaatt agttgccgat catttaatcg cggcaaacct ggcagggcat gattcacatg
     4441 gtattggcat gatcccaagc tatgtacgct cctggagtca ggggcacctg caaattaacc
     4501 atcatgccaa aaccgttaaa gagtcggggg cggcggtcac gctcgatggc gatcgcgcat
     4561 ttggtcaggt cgcggcacat gaagcgatgg cgctggggat tgagaaagcg cataagcacg
     4621 gtattgccgc cgtggcgcta cataactcgc atcatatcgg ccgtatcggt tactgggcgg
     4681 agcagtgtgc agcggcgggg tttgtctcta tccactttgt tagcgtggtc ggtattccaa
     4741 tggtcgcgcc gttccacggt cgcgacagcc gctttggcac caatccgttc tgtgtggttt
     4801 tccctcgtaa agataatttc ccgctgttgc ttgattacgc caccagcgcc attgcatttg
     4861 gtaaaactcg cgtcgcctgg cataaaggcg tccccgtgcc gccaggttgc ctgattgacg
     4921 ttaacggcgt gccgacgacc aatccggcgg taatgcagga gtcgccgttg ggggcgctgt
     4981 tgacctttgc cgaacataaa ggctacgccc ttgcagcgat gtgtgaaatt cttggcgggg
     5041 cgctttctgg cggtaaaacg acgcatcagg aaacgttaca aaccagtccc gatgccattc
     5101 ttaactgcat gaccactatc atcatcaacc cggaactctt cggcgcgccg gattgtaacg
     5161 cgcagaccga agcctttgcc gagtgggtga aagcctcgcc gcatgatgat gataagccga
     5221 ttttgctacc gggcgagtgg gaagtgaaca cgcgtcgcga acggcagaag caggggattc
     5281 cactggatgc gggaagctgg caggccattt gtgatgcagc gcggcagatt ggtatgccgg
     5341 aagagacgtt gcaggctttc tgtcagcagt tagccagcta aaaaaaagcc cgtccagtgg
     5401 cggacgggca aacaagggta acataggatc aatgagggtt agagcatatg cgtctgtcgg
     5461 caaacagaca gggaaatact tgtgctggac gtagcgtaaa cgcctgatcc gtccaacggt
     5521 tcggattttg tagatactga taagcgtagc gcatcaggca gttttgcgtt tgttcatcag
     5581 cctgatgccg ggcatagccc ggcataaaag attactgcgc agcggtaggt ttacgcggac
     5641 ggcggcgacg ttgttgttcg cctgccggtt tggcatcgcc gaggcggcga gacggttttt
     5701 ctgcaggttt cgcgcttgca gattttgcgc caccttcccc acggcgtggt tgctgttgac
     5761 cgcgaccacc accttgccca cgaccgccgt cgccacgttg ctggcgaccg ttctggatcg
     5821 gttcggcttt gattgacggg tccggctcat agcccggaat cgcaatgcgc gggatctctt
     5881 ttttcagcag tttttcgata tcacgcagca gtttgtgttc atcaacacac accagcgaca
     5941 acgcttcacc ggtagcagcc gcacgaccgg tacgcccgat acggtggaca taatcttccg
     6001 gtacgtttgg cagttcatag ttgacgacgt gcggcaactc ttcgatatcc agaccgcgcg
     6061 cagcgatatc ggtcgccacc agtacgcgaa tgtcacccga tttaaaatca gccagcgcac
     6121 gggtacgcgc accttgcgat ttattcccgt ggatcgccgc actacggatg ccatctttat
     6181 tgagctgctc agccagatgg ttagcgccgt gtttggtacg ggtaaacacc agcacctgct
     6241 gccagttccc tttaccaatc atgtgcgaca gcaattcgcg tttgcgtttc ttatcaacaa
     6301 agtgaacgtg ctgggtcacc tgatcagacg cggtattgcg gcgtgccact tcgatttcca
     6361 gcgggttgtg caacagtttt tccgccaggg ctttaatatc gtcagagaag gtcgcagaga
     6421 acagcaggtt ctggcgcttc gctggcagtt ttgttaacac gcgacggata tcgtggataa
     6481 agcccatgtc gagcatgcgg tcagcttcat cgaggacgag gatttcaacc tgatccagct
     6541 tcactgcatt ttgatgttcc aggtccagca aacgtcccgg tgttgccacc agaacatcaa
     6601 cgccgccgcg cagcttcatc atttgcgggt taatgctgac accgccaaac accaccagcg
     6661 aacgaatgtt caaatatttg ctgtaatcac ggacgttttc gccaatctgc gcagccagtt
     6721 cacgggtcgg ggtaagaatg agcgcacgta ccggacgacg ccctttggcg tgcggctggc
     6781 gagtgatcag gtgttgcaac agcggcagcg taaagcccgc tgttttaccg gtaccggtct
     6841 gggcgctagc catcaggtcg cggccttcca gcaccgcagg gatcgcctgc tgctgaatag
     6901 gggtgggttc acggtaaccc tgctcggcaa cggcgcgcag gatatcaggg cttaaaccca
     6961 aagaatcgaa agacataact actccgaacc gccccgaccg ttaccggtgt agttttcagg
     7021 gagatactga aaaggaaaat gacaaaaacc acaatgtcat gagggagcgg agtgtagcag
     7081 tttttgtgac gcagcgcata aattatccct gtgactggcg tagctaaaat tcttagccat
     7141 actggacaac tcccatatgg agtcataatc ttaatcaatc atttgattaa gaatggactg
     7201 tgcgatgaat aatcctgcca tgacaatcaa gggtgaacag gcgaaaaaac agctgattgc
     7261 tgccgcactg gcgcagtttg gtgaatatgg aatgaacgcc accacccgcg agatagccgc
     7321 ccaggccggg cagaatatcg ctgccatcac ctactacttc ggttcgaaag aagatttgta
     7381 cctcgcctgc gcccagtgga ttgccgattt tattggcgag cagttccgtc cgcacgccga
     7441 agaggccgaa cgcttgttcg cacaaccaca gcctgatcgc gcagccatcc gtgaactgat
     7501 ccttcgcgcc tgcaggaaca tgattaagct gctcacccag gatgacaccg tcaacctcag
     7561 caagtttatc tcccgtgagc agctctctcc cacggcagcc tatcacctgg tgcacgaaca
     7621 ggtgattagc ccgctacaca gccacctgac acgcctgatt gccgcctgga ccggctgcga
     7681 cgccaacgat acccgcatga tcctccatac tcatgcgttg attggtgaga ttctggcgtt
     7741 ccgtcttggc aaagaaacaa ttctgttacg taccggctgg accgcgttcg atgaagaaaa
     7801 gaccgaactg atcaaccaga cggtgacctg tcatatcgac ctgattttgc aaggattatc
     7861 gcaaaggagt ttgtagtgat gaaaaaacct gtcgtgatcg gactggcggt agtggtactt
     7921 gccgccgtgg ttgccggagg ctactggtgg tatcaaagcc gccaggataa cggcctgacg
     7981 ctgtatggca acgtggatat tcgtacggtg aatcttagtt tccgtgttgg ggggcgcgtt
     8041 gaatcgctgg cggtggacga aggtgatgct atcaaagcgg gccaggtgct gggcgaactg
     8101 gatcacaagc cgtatgagat tgccctgatg caggcgaaag cgggtgtttc ggtggcacag
     8161 gcgcagtatg acctgatgct tgccgggtat cgcgatgaag aaatcgctca ggccgccgca
     8221 gcggtgaaac aggcgcaagc cgcctatgac tatgcgcaga acttctataa ccgccagcaa
     8281 gggttgtgga aaagccgcac catttcggca aacgacctgg aaaatgcccg ctcctcgcgc
     8341 gaccaggcgc aggcaacgct gaaatcagca caggataaat tgcgtcagta ccgttccggt
     8401 aaccgtgaac aggacatcgc ccaggcgaaa gccagcctcg aacaggcgca ggcgcaactg
     8461 gcgcaggcgg agttgaattt gcaggactca acgttgatag ccccgtctga tggcacgctg
     8521 ttaacgcgcg cggtggagcc aggcacggtc ctcaatgaag gtggcacggt gtttaccgtt
     8581 tcactaacgc gtccggtgtg ggtgcgcgct tatgttgatg aacggaatct tgaccaggcc
     8641 cagccggggc gcaaagtgct gctttatacc gatggtcgcc cggacaagcc gtatcacggg
     8701 cagattggtt tcgtttcgcc gactgctgaa tttaccccga aaaccgtcga aacaccggat
     8761 ctgcgtaccg acctcgtcta tcgcctgcgt attgtggtga ccgacgccga tgatgcgtta
     8821 cgccagggaa tgccggtgac ggtgcaattc ggtaacgagg caggacatga atgatgccgt
     8881 tatcacgctg aacggcctgg aaaaacgctt tccgggcatg gacaagcccg ccgtcgcgcc
     8941 gctcgattgt accattcacg ccggttatgt gacggggctg gtggggccgg atggtgcagg
     9001 taaaaccacg ctaatgcgga tgctggcggg attactgaaa cccgacagcg gcaatgccac
     9061 ggtgattggc tttgatccaa tcaaaaacga cggcgcgctg cacgccgtgc tcggctatat
     9121 gccgcagaaa tttggtctgt atgaagatct cacggtgatg gagaacctca atctgtacgc
     9181 ggatttgcgc agcgtcaccg gcgaggcacg taagcaaact tttgctcgcc tgctggagtt
     9241 tacgtctctt gggccgttta ccggacgcct ggcgggcaag ctctccggtg ggatgaaaca
     9301 aaaactcggt ctggcctgta ccctggtggg cgaaccgaaa gtgttgctgc tcgatgaacc
     9361 cggcgtcggc gttgacccta tctcacggcg cgaactgtgg cagatggtgc atgagctggc
     9421 aggcgaaggg atgttaatcc tctggagtac ctcgtatctc gacgaagccg agcagtgccg
     9481 tgacgtgtta ctgatgaacg aaggcgagtt gctgtatcag ggggaaccaa aagccctgac
     9541 acaaaccatg gccggacgca gttttctgat gaccagtcca cacgagggca accgcaaact
     9601 attgcaacgc gccttgaaac tgccacaggt cagcgacggc atgattcagg ggaaatcggt
     9661 acgtctgatc ctcaaaaaag aggccacacc agacgatatt cgccatgccg acgggatgcc
     9721 ggaaatcaac atcaacgaaa ctacgccgcg ttttgaagat gcatttattg atttgctggg
     9781 tggtgccgga acctcagaat cgccgctggg cgcaatatta catacggtag aaggcacacc
     9841 cggcgagacg gtgatcgaag cgaaagaact gaccaagaaa tttggtgatt ttgccgccac
     9901 cgatcacgtc aactttgccg ttaaacgtgg ggagattttt ggtttgctgg ggccaaacgg
     9961 cgcgggtaaa tcgaccacct ttaagatgat gtgcggtttg ctggtgccga cttccggcca
    10021 ggcgctggtg ctggggatgg atctgaaaga gagttctggt aaagcgcgcc agcatctcgg
    10081 ctatatggcg caaaagtttt cgctctacgg taacctgacg gtcgaacaga atttacgctt
    10141 tttctctggt gtgtatggct tacgcggtcg ggcgcagaac gaaaaaatct cccgtatgag
    10201 cgaggcgttc ggcctgaaaa gtatcgcctc ccacgccacc gatgaactgc cattaggttt
    10261 taaacagcgg ctggcactgg cctgctcgct gatgcatgaa ccggacattc tgtttctcga
    10321 cgaaccgacg tccggcgttg atccgattac tcgccgcgaa ttctggctac atatcaacag
    10381 catggtagag aaaggcgtca cggtgatggt caccacccac tttatggatg aagcagaata
    10441 ttgcgaccgc atcggcctgg tgtaccgtgg gaaattaatt gccagcggca cgccggatga
    10501 tttgaaagcg cagtcggcca acgacgagca acccgatccc accatggagc aagcctttat
    10561 tcagttgatc cacgactggg ataaggagca tagcaatgag taacccgatc ctgtcctggc
    10621 gtcgcgtacg ggcgctgtgc gttaaagaga cgcggcagat cgttcgcgat ccgagtagct
    10681 ggctgattgc ggtagtgatc ccgctgctac tgctgtttat ttttggttac ggcattaacc
    10741 tcgactccag caagctgcgg gtcgggattt tactggaaca gcgtagcgaa gcggcgctgg
    10801 atttcaccca caccatgacc ggttcgccct acatcgacgc caccatcagc gataaccgtc
    10861 aggaactgat cgccaaaatg caggcgggga aaattcgcgg tctggtggtt attccggtgg
    10921 attttgcgga acagatggag cgcgccaacg ccaccgcacc gattcaggtg atcaccgacg
    10981 gcagtgagcc gaataccgct aactttgtac aggggtatgt cgaagggatc tggcagatct
    11041 ggcaaatgca gcgagcggag gacaacgggc agacttttga accgcttatt gatgtacaaa
    11101 cccgctactg gtttaacccg gcggcgatta gccagcactt cattatcccc ggtgcggtga
    11161 ccattatcat gacggtcatc ggcgcgattc tcacctcgct ggtggtggcg cgagaatggg
    11221 aacgcggcac catggaggct ctgctctcta cggagattac ccgcacggaa ctgctgctgt
    11281 gtaagctgat cccttattac tttctcggga tgctggcgat gttgctgtgt atgctggtgt
    11341 cagtgtttat tctcggcgtg ccgtatcgcg ggtcgctgct gattctgttt tttatctcca
    11401 gcctgttttt actcagtacc ctggggatgg ggctgctgat ttccacgatt acccgcaacc
    11461 agttcaatgc cgctcaggtc gccctgaacg ccgcttttct gccgtcgatt atgctttccg
    11521 gctttatttt tcagatcgac agtatgcccg cggtgatccg cgcggtgacg tacattattc
    11581 ccgctcgtta tttcgtcagc accctgcaaa gcctgttcct cgccgggaat attccagtgg
    11641 tgctggtggt aaacgtgctg tttttgatcg cttcggcggt gatgtttatc ggcctgacgt
    11701 ggctgaaaac caaacgtcgg ctggattagg gagaagagca tgtttcatcg cttatggacg
    11761 ttaatccgca aagagttgca gtcgctgctg cgcgaaccgc aaacccgcgc gattctgatt
    11821 ttacctgtgc ttattcaggt gattctgttc ccgttcgccg ccacgctgga agtgactaac
    11881 gccaccatcg ccatctacga tgaagataac ggcgagcatt cggtggagct gacccaacgt
    11941 tttgcccgcg ccagcgcctt tactcatgtg ctgctgctga aaagcccaca ggagatccgc
    12001 ccaaccatcg acacacaaaa ggcgttacta ctggtgcgtt tcccggctga cttctcgcgc
    12061 aaactggata ccttccagac cgcgccgttg cagttgatcc tcgacgggcg taactccaac
    12121 agtgcgcaaa ttgccgccaa ctacctgcaa cagatcgtca aaaattatca gcaggagctg
    12181 ctggaaggaa aaccgaaacc taacaacagc gagctggtgg tacgcaactg gtataacccg
    12241 aatcttgact acaaatggtt tgtggtgccg tcgctgatcg ccatgatcac cactatcggc
    12301 gtaatgatcg tcacttcact ttccgtcgcc cgcgaacgtg aacaaggtac gctcgatcag
    12361 ctactggttt cgccgctcac cacctggcag atcttcatcg gcaaagccgt accggcgtta
    12421 attgtcgcca ccttccaggc caccattgtg ctggcgattg gtatctgggc gtatcaaatc
    12481 cccttcgccg gatcgctggc gctgttctac tttacgatgg tgatttacgg tttatcgctg
    12541 gtgggattcg gtctgttgat ttcatcactc tgctcgacac aacagcaggc gtttatcggc
    12601 gtgtttgttt ttatgatgcc cgccattctc ctttccggtt acgtttctcc ggtggaaaac
    12661 atgccagtat ggctgcaaaa cctgacgtgg attaacccta ttcgccactt tacggacatt
    12721 accaagcaga tttatttgaa ggatgcgagt ctggatattg tgtggaatag tttgtggccg
    12781 ctactggtga taacggccac gacagggtca gcggcgtacg cgatgtttag acgtaaggtg
    12841 atgtaacttc ttatctttcg ccagcaaaga cactaccgcc gggccggcaa ggattgccag
    12901 ccctgcaatt gccagcaaaa agttgttttc cagtacacgg gacaacagcc acagcacaat
    12961 cagcgctgcg ccaaaatacc aggtcgtggt cagctcttcc agcacgtctg ccacctcacg
    13021 ccagcgttgc tcggggtggc gctgaaacac caacatcatc actaccgtga cacaatacag
    13081 tgcgcataat cccacgccga cgtgaaccag agtcttactc atccagccca ccaccagcag
    13141 cgccactacc agtaaatgca acataatctg ccagcaactt agaccagttg cgacacgaac
    13201 acgttgttgc cacttcatgg cttctctcct gaagaattta tctctactta ctcagagtac
    13261 cgaactttac ggaaaattcc gtaagaccgc acctttttgt gacaccgact acactatttc
    13321 aatgagataa gaggcaaaga caggaaaatt atgcccgatc aaacacaaca attttcgttc
    13381 aaggtgctca ccatcaacat tcacaaaggt tttaccgcgt ttaaccgacg cttcattttg
    13441 ccggaacttc gcgacgcggt gcgtaccgtc agcgccgata ttgtttgcct gcaggaagtg
    13501 atgggcgcgc acgaagttca tccgctgcat gtggaaaact ggcccgatac ctcgcactac
    13561 gagtttctcg ccgacacgat gtggagcgat tttgcctacg gtcgcaatgc cgtatacccg
    13621 aaagggcatc acggcaacgc cgtactgtcg cgttatccca ttgaacatta tgagaatcgc
    13681 gatgtttcgg tcgatggtgc ggaaaagcgc ggcgtgctct actgccgcat tgtgccaccg
    13741 atgaccggaa aagcgattca tgtgatgtgc gtacatctgg gcctgcgtga gacgcaccgt
    13801 caggcgcagc ttgcgatgct cgccgaatgg gtgaatgagc taccggacgg cgaaccggta
    13861 ttggtggcgg gtgatttcaa tgactggcgg caaaaagcta atcatccgtt aaaagtgcag
    13921 gccggactgg atgagatttt tacccgcgcc cacggacgcc cggcgcgcac gtttccggtg
    13981 caatttcctc tactacgact ggacaggatc tacgtcaaaa atgccagcgc cagcgcgcca
    14041 accgcgttgc cgctgcggac atggcgacac ctttctgatc atgccccttt aagtgcggag
    14101 attcatttat gaaatgtagc tggcgcgaag gcaataagat ccagttgctg gaaaacggcg
    14161 agcaatatta tcccgcggtg tttaaggcga ttggcgaggc acaagaacgc atcattcttg
    14221 aaacgtttat ctggtttgag gatgacgtcg gcaaacagct gcatgcggca ctactggcag
    14281 cagcgcaacg cggggttaaa gcggaagtct tgctggatgg ctacggttcg ccggatctca
    14341 gcgatgagtt tgtcaatgaa ctgacggcag ctggcgtggt atttcgctac tacgatcccc
    14401 gccctcgcct ttttggtatg cgcaccaatg tgtttcgccg gatgcatcgc aaaattgtgg
    14461 tgatcgacgc gcgtatcgcc tttattggcg ggctgaatta ctccgccgag catatgtcca
    14521 gctacggtcc agaggctaaa caggattacg cggtacgcct tgaagggccg attgtcgaag
    14581 atattctcca gtttgagctg gaaaacctgc ctggacagag cgcggcacga cgctggtggc
    14641 gacgtcatca caaagcggaa gagaaccgcc agccgggaga agcgcaggta ttgctggtct
    14701 ggcgcgataa cgaagaacat cgcgatgata ttgaacgtca ctatctgaaa atgctcactc
    14761 aggcgcggcg agaagtcatt atcgccaacg cctacttctt ccccggctat cgatttttac
    14821 acgccttgcg taaagcggca cggcgcgggg tgcggatcaa actgatcatt cagggcgaac
    14881 cggatatgcc gattgtcaga gtcggtgcgc gcttgctgta taactatctg gttaaaggcg
    14941 gcgttcaggt atttgagtac cgccgccgcc cgctccacgg caaagtggca ttgatggacg
    15001 atcactgggc gacagtaggg tccagtaatc tcgatccgct cagtttgtca ctgaatctcg
    15061 aagcaaatgt catcatccac gatcgtcatt ttaaccagac gctgcgcgat aatctgaacg
    15121 gcattattgc cgcagattgt cagcaggtcg atgagaccat gctgccgaaa cgcacctggt
    15181 ggaacctgac caaaagcgtg ctggcgttcc actttttacg ccacttcccg gcgctggttg
    15241 gctggcttcc ggcacacaca ccacgtctgg cgcaggttga tccgcccgca caaccgacaa
    15301 tggaaacgca ggatcgggta gaaactgaaa acacgggggt aaaaccctga tgagtaaatc
    15361 acacccgcgc tggcgcttag caaagaagat cctcacctgg ctgtttttta tcgcggtgat
    15421 tgtgttgctg gtggtctacg ccaaaaaagt ggactgggaa gaggtctgga aggtcatccg
    15481 cgactacaat cgcgttgcgc tgcttagtgc ggtcgggctg gtggtcgtca gctatctgat
    15541 ttacggctgc tatgacctgc tcgcccgctt ttactgcggt cacaaactgg cgaagcgcca
    15601 ggtgatgctg gtgtcgttta tctgctacgc cttcaacctg acgctcagta cctgggtcgg
    15661 cggcattggt atgcgctatc gtttgtactc ccgactaggg ttaccgggca gcactattac
    15721 gcggattttc tcgctcagta ttaccaccaa ctggctgggc tatattttac tggcagggat
    15781 tatctttacc gcaggcgtgg tggagttgcc ggatcactgg tatgtcgatc aaactacgct
    15841 gcgtattctc ggcattggct tactgatgat tatcgcggtt tatttgtggt tttgcgcttt
    15901 cgcgaagcac cgccatatga ccatcaaagg gcaaaagctg gtgctgcctt catggaaatt
    15961 cgccctcgct cagatgctga tttccagcgt taactggatg gtaatggggg cgattatctg
    16021 gctgttactt ggtcaaagcg tgaactattt ctttgtactg ggcgtgttac tggttagtag
    16081 tattgctggc gtcatcgtgc atattccggt ggggatcggt gtgctggaag cggtgtttat
    16141 cgcactactg gctggggagc atacctcgaa agggagcatt atcgccgccc tgctcgctta
    16201 tcgcgtcctg tattacttta tcccgctgct gctggcgctg atctgttacc tgctactgga
    16261 aagccaggcg aagaagctac gggcgaaaaa tgaagcggcg atgtgaagtt tagttaagtt
    16321 ctttagtatc tgcatttacg actaatgaaa aaaacgcgta tgcctttgcc agacaagcgt
    16381 tatagctatc gcggcaagta tcccaaagaa gccgataaaa tagtaataga gtgttaatgc
    16441 acctgtaaca gcgcaacgaa ccggactcca caactcttca ctatggcatt tccccgccaa
    16501 ttcaaggagc ttatttcgtc catgcgagat aatgcctgac cataacacaa tcattaagca
    16561 acttattacc caaacgggtc gttcactcat taatgcggca ttcatgatga tcaccagggc
    16621 caaccctgtc aacgtcatca taatgataaa acggtgagaa ccaggatcaa cattaaataa
    16681 taaaccggcc agcatactga ttaaaaacat cgcgcccgca gtgctgaaca cgacgataat
    16741 ggcaggtaca ggagctaaac ttgcaacgac aatcgccacc aaagagagta aaaacagata
    16801 tattacgcct gcgatgacgg catttatctt gttgatatcc ttacttaagc catacataca
    16861 aactaataat aaccctatgg cgacatacat tgttggtgaa tcatatttgg ttgccgtaat
    16921 ttcgagatac ttagctcccc aggccagtcc tgcggttgcc accaaaccta aagcggtaat
    16981 gagccatata ccgtttaata tcctggtctc gtgctgaaaa tctaatttat tgctgttttg
    17041 tgagtatgat tccattcttt tagttg
//
LOCUS       DAZKGS010000096        16928 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKGS010000096 DAZKGS010000000
VERSION     DAZKGS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..65
                     /locus_tag="ACHOQY_003125"
     CDS             <1..65
                     /locus_tag="ACHOQY_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679178.1"
                     /translation="LHYIDTGAVFGGELTLVQLQ"
     gene            complement(66..257)
                     /gene="yebW"
                     /locus_tag="ACHOQY_003126"
     CDS             complement(66..257)
                     /gene="yebW"
                     /locus_tag="ACHOQY_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9679179.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(362..598)
                     /gene="yebV"
                     /locus_tag="ACHOQY_003127"
     CDS             complement(362..598)
                     /gene="yebV"
                     /locus_tag="ACHOQY_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9679180.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(716..2155)
                     /gene="rsmF"
                     /locus_tag="ACHOQY_003128"
     CDS             complement(716..2155)
                     /gene="rsmF"
                     /locus_tag="ACHOQY_003128"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9679181.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(2235..4868)
                     /gene="yebT"
                     /locus_tag="ACHOQY_003129"
     CDS             complement(2235..4868)
                     /gene="yebT"
                     /locus_tag="ACHOQY_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9679182.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4837..6120)
                     /gene="yebS"
                     /locus_tag="ACHOQY_003130"
     CDS             complement(4837..6120)
                     /gene="yebS"
                     /locus_tag="ACHOQY_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9679183.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6250..6747
                     /gene="msrC"
                     /locus_tag="ACHOQY_003131"
     CDS             6250..6747
                     /gene="msrC"
                     /locus_tag="ACHOQY_003131"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9679184.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6844..7542
                     /gene="proQ"
                     /locus_tag="ACHOQY_003132"
     CDS             6844..7542
                     /gene="proQ"
                     /locus_tag="ACHOQY_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9679185.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7562..9610
                     /gene="prc"
                     /locus_tag="ACHOQY_003133"
     CDS             7562..9610
                     /gene="prc"
                     /locus_tag="ACHOQY_003133"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9679186.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            9802..10683
                     /gene="htpX"
                     /locus_tag="ACHOQY_003134"
     CDS             9802..10683
                     /gene="htpX"
                     /locus_tag="ACHOQY_003134"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9679187.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10729..12102)
                     /gene="yebQ"
                     /locus_tag="ACHOQY_003135"
     CDS             complement(10729..12102)
                     /gene="yebQ"
                     /locus_tag="ACHOQY_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679188.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            12279..13070
                     /gene="kdgR"
                     /locus_tag="ACHOQY_003136"
     CDS             12279..13070
                     /gene="kdgR"
                     /locus_tag="ACHOQY_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9679189.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13213..13452)
                     /gene="yobH"
                     /locus_tag="ACHOQY_003137"
     CDS             complement(13213..13452)
                     /gene="yobH"
                     /locus_tag="ACHOQY_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9679190.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13611..13754
                     /gene="mgrB"
                     /locus_tag="ACHOQY_003138"
     CDS             13611..13754
                     /gene="mgrB"
                     /locus_tag="ACHOQY_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9679191.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13829..14116
                     /gene="yebO"
                     /locus_tag="ACHOQY_003139"
     CDS             13829..14116
                     /gene="yebO"
                     /locus_tag="ACHOQY_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9679192.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            14785..14928
                     /gene="yobF"
                     /locus_tag="ACHOQY_003140"
     CDS             14785..14928
                     /gene="yobF"
                     /locus_tag="ACHOQY_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9679193.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14941..15150
                     /gene="cspE"
                     /locus_tag="ACHOQY_003141"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14941..15150
                     /gene="cspE"
                     /locus_tag="ACHOQY_003141"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9679194.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15316..16125
                     /gene="rlmA"
                     /locus_tag="ACHOQY_003142"
     CDS             15316..16125
                     /gene="rlmA"
                     /locus_tag="ACHOQY_003142"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9679195.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16122..16688)
                     /gene="mntP"
                     /locus_tag="ACHOQY_003143"
     CDS             complement(16122..16688)
                     /gene="mntP"
                     /locus_tag="ACHOQY_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9679196.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4445 a         4129 c         4291 g         4063 t
ORIGIN      
        1 acctgcatta tattgatacc ggtgctgtct ttgggggcga actgactctt gtgcaattgc
       61 aataattaaa aatcaccata ctcctgtgct ggtcgccaga aaccatctat aaaatcctca
      121 atcggaaaac aaccgccatg gcggatccgt tgatcgctca tagaataaag acactgctgt
      181 tccgtgttgt agacatccac aacaatatct tcacaaccgc catccaggta gcaaacaaaa
      241 agtaccagcg cgaacatttc atccccgaag tgtggtgccg taccgttaag tttaggagag
      301 attttacaac gggggaataa ccaggacaaa taacccgcca tgccgacggg ttctttttgg
      361 atcaggcaag acgcataatc caggcatcag attgttgatc gtaacgggta cggtaaagga
      421 cagagtgttc gccatttagc agggcaggga tactggtttc agcatcccag gcatcgagtt
      481 gcatacataa atcggggtca gatttacaag gaatggttaa cgttcgatca cccggcgatt
      541 caccgtgtaa ttcgccgtcg tcgatttcaa aagcgcctat gcgcacactg gttttcatct
      601 tcgtgctctc catttagctg ccgattgtgg tacgaccagt ccgatcgcac catttttcag
      661 cctagttgag gcagccgaat gtgccagtca aaaagtgcgc tttttttgtg agctgtcagg
      721 cgttaccggt aaaaagtttc ccatcgcgca ccagttcacg cggatagctg tttttcaatc
      781 gcgaaccaat ccgtttggct aaaccaatcg gttgatgctg gaaagtaacc aatacatcat
      841 ccgccactgg cgcggcttgc gggtaaacat cgcgcccgcg ataccactcc tccgcttcct
      901 gcggtgtcag ctcgaatgca ttcatattat cgggtgaggc aagagcaata accgcttcat
      961 gctgccagcg ataacctttg ttgtgcgtct cggcaagttt aatccccaac cgagaaaatc
     1021 ggactttacc gatcagggct tcaatgccca ccgggaacaa ccacagttct ttgtcacgct
     1081 gccagaggcg caggttttca tcccagttta agccaacacc tgcagtcgcc tgacgaattt
     1141 gtccagcttc gcgatctttc accgggctga acggaaaatt accgactttg tatttggggg
     1201 cgggtaaggc gggaatcgcc tgagttttac gcagacgagc aacgaagaag ccttcgcagt
     1261 cgtaaatttg tgggaaaaca tgcaaaaagc cttcttcggt cagcgctttg tttgcaccag
     1321 ggaagagatc gccaagtggt aaaaactcta ctgcgtcggg gtaagtctct ttcagccaca
     1381 ggcaaacggc ttcgttttct tcctggttta aggtacaggt cgagtaaacc agcgtaccac
     1441 caggacgtaa tgcatgaaag gcgctgtcga taagctcccg ttgtgtagct gcgatttcct
     1501 gattgctttc tggtgaccag ttttttagcg catcgggatc tttacgcacc acgccttcgc
     1561 cagagcaggg agcgtccagc aaaatggcat cgaacatttc tggcactgcc gcaccaaaca
     1621 cgcggccatc aaaatgtgtg agcgcaacat tactgatgcc acagcggctg atattggcat
     1681 gtaacacttt tacccgactg gcggaaaact cattggcaag gattgcccct tcgttattca
     1741 tccgcgcggc aatttgcgtc gttttggagc ctggcgcagc tgcgacatcc atcacccgct
     1801 gtggtgcatt atcgtcagca aacaaggcgg caacgggcaa cattgaactg gcttcctgaa
     1861 tataaaacag gccgcttaaa tgctcggcgg tactacccaa tggcaatgca tcttcattgt
     1921 cgcgttcaat ccagaaacct tcttcacacc acggaattgg cgtaagcgtc cagccataag
     1981 gagcggttaa ttgcaggaaa tcagcaacgg agattttcag cgtattaacg cgaatgctgc
     2041 ggcgcaacgg gcgctgacag gcggcaagaa aatcatcaaa tgagagcgtc gaaggcatcg
     2101 cttcgcgcat ttgtgtcaga aaggcgtccg ggaaataaac ggtgtgttgg gccacgagca
     2161 tgtaccactg gcaaaaaaac aggcgcgcag tttagcataa acgctccggc gcaggcacgc
     2221 cggagcagtg ggcattattt gggaagcgca gttccccatt cacgccactc tttcggttca
     2281 ctttcctgca acaggaagtg tttgccttcc tgggctttcg gtgccagtgg cgtacccgga
     2341 ggcgtggcga aggcgatgcc gccacggata aactggttaa aggtgccggt tttcactacg
     2401 ccgcccgtca gaccaaagtc cagactgtaa cccgatgcca accagaagac ggaattgtta
     2461 cgcaccaggt gttgatagcg tttactgatg cgcatcgcaa tcatcacgcg atctgacaat
     2521 gtccccagcg tcattcctgt aaccgtaccg acttccagac cacggaacag cacaggcgta
     2581 ccaataccta acgaaccggc ttccggcgct tcaacaataa tgcttaagcc atccaggtaa
     2641 cgcgaatcag taatggtggc ctcttgtaat tcaaagtcgc ggcgaggatt gccccggcct
     2701 ggttcgacgt tgatatacgg ctggaggata gtatctagat gctcaacgcc cgcggctgaa
     2761 atttgcggtg tgaccaccga gaagcgcgta ccgccgcggg cgaaggtctg gacatactcc
     2821 ggatagagca ccgcctttgc ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg
     2881 atttgcccga tatcaatacc gagatagcga attggcatcc cgaccgccag ttttccggca
     2941 tcgaaagcgt gaagcgtaat ctgcccacca accgcacggg ccgctgtttc ggaagcatac
     3001 agaattcgtt tgtcgccttt acgctgactg gcgctggcac cgctgaggtt atcgaagcta
     3061 atggctccct ttaatgctct ggagagcggg gatgcctgta cggtcaggcc actaccattc
     3121 agctgaactt tcgccccgcc ttctgcccag aacacgctat tgctggtcag aaggttgcga
     3181 tactccggct taatatgcag atcgatatca aacgcgttag ctcgcggacg gacggtaata
     3241 acttcaccaa cttcaaattt acggtagagc actaccgatc ctgcctgcac atccggcagc
     3301 gtctctgcac tcaaactcac ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct
     3361 ttttccagat tggcatacag tggatagctg gctttcatct cgcctttatc gcccggcaga
     3421 atacgtatcc cgccgttaat ccattctgag gcgctggcac cgagaaactc aacgccatcc
     3481 agccccacct tcacgtcgac acggctgttg acgacaaatt tgctatcgcc ttttaccagt
     3541 tctcgatgct gaggctcgat ggcgacggta aaggtgacgc ctttgctggt gagtttacga
     3601 tcgataacct ggcctacctg cacgccgtga agaatgagcg gctgaccagc atcaataccg
     3661 taactttccg gcgcggtcag ggtcagcgtc agaacatcag gttcatgcag cagtgctttt
     3721 tcgcctggca caacaacgaa ctctttgcgt ggctcgccat cgccgggtac caactcgaag
     3781 gttttgccag tcagcagggc gctgagattg gcatcgctaa gggataattt cggattacgt
     3841 aattcgatgc gggtattttc acgaagcagg gtaacgacgc tgggatcaac tgtcatttcc
     3901 ccagtgactt taccaccagg atttaaatcc agtttagtta gctgtccgac ttccagcccc
     3961 tgatacatta acggcgtcga gtcggcggtc aatccggccc cactcggcag ttccagtttt
     4021 attattacgc cacgctggct gtgggccaga tcttcataca gaccaaaggt atcttccgcc
     4081 tcggcaggtt tcgactcttc aggtgaatcg aaggcaatcg caccgttaac cagtgccgcc
     4141 agactttcca gtttcacctt cgcgccactg acactgacgt tggcatcaac gccggaaacg
     4201 ttccagaaac ggctaccttt tttcaccaga tcggtaaaac gccgctcgat caggacatca
     4261 atcaccacgc cttgcttgtt gggattgatg gcatagtcgt agacttttcc caccgggatc
     4321 ttgcggaaat agaccaatga accgctgttc agcgaaccga gatcgggagc ttgcaggtgg
     4381 atcatcagat cgccgttatc cagccgatac ttcggttggg tatcgagtgc gacaaagtga
     4441 tcctgctctt tacctttacc cggcatcatg ccgatatagt tcccaccgac gagggcgtcc
     4501 agcccggaga cacctgccaa cgatgctttt ggcgtcacca gccagaattg agtctcttcg
     4561 cgcagcgcat ctttcatatc ggacttgatg ctgaccttga cttcaatctt acgaagatcg
     4621 tcgctgaggc tgatatcctg cactgttccg acttcaacgc cctgataacg aacaggcgta
     4681 cggcccggaa caataccatc cgccgacata aagtcgatgg tgacggtatt accccggtcc
     4741 tgataactgt cccaaatcag ccaaccggca atcattagcg cgatgaaagg cagcagccag
     4801 aaaggtgaga tacggcgttt atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc
     4861 tgactcatgt gcatcccaaa gtaagcggct gtccagccat tccacagcaa gaatagtcaa
     4921 aattacggct gcgccaaaat aaaacgcagc cggtcccata gtaaaagcga ggatctgatc
     4981 gcgattaatc agcgacatgg ttaaagatat gacaaacagg tcgagcatcg accagcgtcc
     5041 aatccaggtc accatccgca gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa
     5101 atgaatgctc aacagtaaag tgaacatgac gatcacttta gtaaacggta ccagaatact
     5161 ggcgataaac acgattcccg caaccgcaat gttgctactt gccagcgaca taattccgga
     5221 aagaattgta tcttcctgcc gtcctccgtt cagataaatg atagaaatag gcaataggtt
     5281 ggctggtaac aacaaaacga ttgacgctaa cagcgccgcc cagcattttt gcagactatg
     5341 acggcgacgc aggcgtagcg ggatatggca acgcgggcag cgaccacgct gatcaggata
     5401 gccggtaaaa tggcacccaa gacagacacg aagtttctcg tccctacgcg tagcggggcg
     5461 ctgcggataa tatcgctccc acagctcctc aacattaaga tgtgacaacg tcaccgtcgt
     5521 taaaatcacc aacgccacaa aagagaacaa gccgacacca gcctggatat gggcataatc
     5581 ctgtaccttt atagaagcaa cgccaatgcc gaccaggtag atatccagca ttacccactc
     5641 tttaagacgc tcaagcatca gcagtaccgg acgtaaattc attcccagtc ggttaccaaa
     5701 ccataaatat gctatggagg acaccagaat gaggggggca ccaataacgc agaaaaagac
     5761 catcgccccc gttatcgtat cgccctgttt ggtcatttgc cagatgcctt gcatgacgtt
     5821 ggcgtcgata cgaataccta acagccagat atgcaacagc ggttcgcccc aggcaaacgg
     5881 catcagcaac agcatggtga aagccattgc cgccaggcgc gttagcgacc agtcgcgccc
     5941 gtcgcgaatt tttgcctgac agcgcggaca ataggcactc tgatgagaat ttatctcggg
     6001 caggctaaac agcatgtcac attgcgggca acgttggtaa tcaccacgcg gcagttcctc
     6061 gccgattgcc ctcactgtta tcttttttgt cggcgtaatt tgtggtgtgt taagagccat
     6121 gacgtgccaa atgtaataag aattaacatt aatattaacc catgattgga atgatcttga
     6181 gcattaacgc atttaatgct tattttaata ggctggcagt tccaggtaag acaaccaagt
     6241 gattatataa tgaacaaaac agaattttac gcggatttaa atcgcgactt taacgcgctg
     6301 atggcgggag aaaccagttt tctggcaacg cttgcgaaca ccagtgcgtt gttatacgag
     6361 cgtctcactg acgtaaactg ggcaggtttt tatttgcttg aggacgatac actggtacta
     6421 ggaccatttc agggcaaaat tgcctgtgtc cggatacccg tcgggcgcgg ggtgtgcggc
     6481 actgcggttg cccgcaatca agtgcagcgt atcgaggatg ttcacgcgtt tgacgggcat
     6541 attgcctgtg atgcggcgag taattctgaa attgttctgc cgctggtggt gaaaaatcag
     6601 attattggtg ttcttgacat cgatagtacc gtcttcggtc gctttacaga cgaggacgag
     6661 caaggcttac gtcagcttgt ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa
     6721 aaattctttg cgagtgtcgc aggataatca acggataacg tagcatttac tggtagcgtc
     6781 attataatga cgcctgttca tgcctgcgct tgttggctac gtccgttgta atcaggaaat
     6841 ttcatggaaa atcaacctaa gttgaatagc agtaaagaag taatcgcgtt tctggccgaa
     6901 cgttttcccc actgtttcag tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt
     6961 caggatttgg tcgatcgtgt tgccggggaa atgaacctga gcaaaacgca attacgatcc
     7021 gctttacgtc tctacacttc gagctggcgt tatctttacg gtgttaaacc cggcgcaacg
     7081 cgtgtcgatc ttgacggcaa cccatgcggt gagctggacg agcaacatgt agagcatgct
     7141 cgcaagcagc ttgaagaagc gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg
     7201 aaaaaacgcg aagctgccgc agctgctggt gagaaagaag acgcaccgcg ccgcgaacgc
     7261 aagccacgtc cgaccacgcc acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa
     7321 aagccggtag agaaagcgcc aaaaacagta aaagcacctc gcgaagaaca gcacacccca
     7381 gtttctgaca tttcagctct gactgtcgga caagccctga aggtgaaagc gggtcaaaac
     7441 gcgatggatg ccaccgtatt agaaatcacc aaagacggcg tccgcgtcca gctgaattcg
     7501 ggtatgtctt tgattgtgcg cgcagaacac ctggtgttct gaaacggagg ccgggccagg
     7561 catgaacatg ttttttaggc ttaccgcgtt agctggcctg cttgcaatag caggccagac
     7621 cttcgctgta gaagatatca cgcgtgctga tcaaattccg gtattaaagg aagagacgca
     7681 gcatgcgacg gtaagtgagc gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt
     7741 cgacctcgat caggcatttt cggccaaaat ctttgaccgc tacctgaatc tgctcgatta
     7801 cagccacaac gtgctgctgg caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt
     7861 aggcgatgaa ctgcgttcag gcaaactcga cgttttctac gatctctaca atctggcgca
     7921 aaagcgccgt tttgagcgtt accagtacgc tttgtcggta ctggaaaagc cgatggattt
     7981 caccggcaac gacacttata accttgaccg cagcaaagcg ccctggccga aaaacgaggc
     8041 tgagttgaac gcgctgtggg acagtaaagt caaattcgac gagttaagcc tgaagctggc
     8101 aggaaaaacg gataaagaaa ttcgtgaaac cctgacacgc cgctacaaat ttgccattcg
     8161 tcgtctggcg caaaccaaca gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg
     8221 tgaaatcgac ccgcatacca actatctttc cccgcgtaat accgaacagt tcaacactga
     8281 aatgagtttg tcgctggaag gtattggcgc agtgctgcaa atggatgatg actacaccgt
     8341 tatcaattcg atggtggcag gtggtccggc agcgaagagt aaagctatca gcgttggtga
     8401 caaaattgtc ggtgttggtc aaacaggcaa gccgatggtt gacgtgattg gctggcgtct
     8461 tgatgatgtg gttgccttaa ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt
     8521 acctgctggt aaagggacca agacccgcac tgtaacattg acccgtgaac gtattcgtct
     8581 cgaagaccgc gcggttaaaa tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct
     8641 ggatattccg ggcttctatg tgggtttgac agacgatgtc aaagttcaac tgcagaaact
     8701 ggaaaaacag aatgtcagca gtgtgatcat cgacctgcgt agcaatggcg gtggggcgtt
     8761 gaccgaagcg gtatcgctct caggtctgtt tattcctgcg ggtcccattg ttcaggtccg
     8821 cgataacaac ggcaaggttc gtgaagacag cgataccgac gggcaggtgt tctataaagg
     8881 cccgctggtg gtactggttg accgtttcag tgcttcggct tcagaaatct ttgccgcggc
     8941 aatgcaggat tacggtcgtg cgctggttgt gggtgaaccg acgtttggta aaggcaccgt
     9001 tcagcaatac cgttcattga accgtattta cgatcagatg ttacgtcctg aatggccagc
     9061 gctgggttct gtgcagtaca cgatccagaa attctatcgc gttaacggcg gcagtacgca
     9121 acgtaaaggc gtaacgccag acatcatcat gccgacgggt aatgaagaaa cggaaacggg
     9181 tgagaaattc gaagataacg cgctgccgtg ggatagcatt gatgccgcga cttatgtgaa
     9241 atcaggcgat ttaacggcct ttgggccgga gctgctgaag gaacataatg cgcgtatcgc
     9301 gaaagatcct gagttccaga acattatgaa ggatatcgcg cgcttcaacg ctatgaagga
     9361 caagcgcaat atcgtttctc tgaactacgc tgtgcgtgag aaagagaata atgaagatga
     9421 tgcgacgcgt ctggcgcgtt tgaacgaacg ctttaaacgc gaaggtaaac cggagttgaa
     9481 gaagctggat gatctaccga aagattacca ggagccggat ccttatctgg atgagacggt
     9541 gaatatcgca ctcgatctgg cgaagcttga aaaagccaga cccgcggaac aacccgatcc
     9601 cgtcaagtaa tatcaatcag gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat
     9661 gccgtaaatc agatgctaca aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc
     9721 cagacttgaa aatagtcgcg taacccatac gatgtgggta tcgcatattg cgttttgtta
     9781 aactgaggta aaaagaaaat tatgatgcga atcgcgctct tcctgctaac gaacctggcc
     9841 gtaatggtcg ttttcgggct ggtactgagc ctgacaggga tacagtcgag cagcgttcag
     9901 gggctgatga tcatggcctt gctgttcggt tttggtggtt ccttcgtttc gcttctgatg
     9961 tccaaatgga tggcattacg atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa
    10021 agggaacgtt ggctggtcaa tactgtagca acccaggctc gtcaggcagg gatcgctatg
    10081 ccgcaagtgg ctatctacca tgcgccggac atcaacgctt ttgcaaccgg tgcgcgccgt
    10141 gatgcctctc tggttgctgt cagcaccggt ttgctgcaga acatgagccc ggatgaagcc
    10201 gaggcggtaa ttgctcacga aatcagtcac atcgccaatg gtgatatggt taccatgacg
    10261 ctgattcagg gcgtggtgaa caccttcgtt atctttattt cccgtattct ggcgcagctt
    10321 gccgcgggtt ttatgggcgg aaatcgtgat gaaggtgaag agagcaacgg caacccgctg
    10381 atctactttg cggttgcaac ggttctggaa ctggtgtttg gtattctggc gagcattatc
    10441 accatgtggt tctcgcgtca tcgtgaattc catgctgatg ccggttcggc aaaactggtt
    10501 ggtcgcgaga aaatgattgc cgcgctgcag cgcctgaaaa ccagctatga accgcaagaa
    10561 gcaaccagca tgatggcttt ctgcattaac ggtaagtcga aatcgctcag tgagttgttc
    10621 atgactcacc cgccgctgga taaacgaatt gaagctctgc gtacgggtga atacctgaag
    10681 taatatgaag taaatactaa aaggcgcgtc ctgatcgacg cgcttttttt atgccctgga
    10741 tcgtggctga gtgatacgta aaccactcac acaggcagca atcactgcca gaatagccgc
    10801 agccatcagc gaaacgtgcg taccattatc accgaactga tttagcatca gcgccaccag
    10861 cgccgcgcca ctactctgac ccagtagacg agccgttcct aacatgccac tggccccacc
    10921 gctacgttcg cgcggagcgg aggtaataat ggtgtggtta ttgggtgact ggaataagcc
    10981 aaatccagcg ccacataaga tcatcggcca gataatattg atatccgcag gtgacgcggg
    11041 cagcagaacc agggaaaaaa gccccgcagc catgatgaac aaccctaaag cacccagcaa
    11101 tcctgcatgt acgcgttcaa tcaaatagcc tgccagcgga gccatcacca tcgttgctaa
    11161 cggccacggt gtcagaagta aacctgtttc gacttcacta cgcccgagca cggtttgcag
    11221 gtaaaagggc agggaaacca ttgccagcat ttgtgcgcag aaagagcaaa cagatgtgca
    11281 aatagaaagt gaaaacagcg ggatacgcag taaatccacc ggtagcagcg gtacgggaag
    11341 agaaagctgg cggcgaataa agaaaatacc aacaacaacc attaccacca gttccgcagc
    11401 aattaacgtc agcgattgcc cctgagcgaa accactcaac gcagtgataa gcaggccgaa
    11461 ggttaacgcg ttcatcacgg cgctgggcag gtcgaaacgg ggtttactgg cgcgagaacc
    11521 attgggtggc agaaaacgca tcgccagaag cagggcgata atacctaacg gtacgttgat
    11581 taaaaataac catttccagg atgcgatgga gaggattgct gcagcaattg tcggcccggc
    11641 agcagaagaa acggcaacaa taaacgagtt tatgcccatc cctctcccca gaaaacgttg
    11701 tggatagatc aggcggataa gtgcggtatt aacgctcatc aacgctgcac cgccgaaacc
    11761 ttgtatgaca cgcgcaaggg tgagcatttg cagcgaatca gaaagggcgc agaacagtga
    11821 agacaacaga aaaacgacca gaccgcattt ataaatacgt cgatagccaa acatatcgcc
    11881 cagaaacgaa aacgagagca gggagatgac aatggcgatt tgataggcgt tcactaccca
    11941 gatggatctg gctggcgtgg catgaaggtc cgtggcgatt gttggcaggg cgacgttggc
    12001 gattgcgccg tcaaggacag ccatcgaaat gccaatcaca atggttaata tcgcaccgta
    12061 tcgctggggc aatggcaggc cgtcggcctg aacttttggc ataggaattt tatatctttg
    12121 gtgaataatc aacaggggta tgattctagc actgatattc acggtggatg tcgcagaatt
    12181 gtaactaacc ggcaaaagat ggattgcgac caccctggtg cgaaattata ataaaaaaca
    12241 gttctgattt ttataaaaca ctcgcaatga ggtgataaat ggctaacgca gatctggata
    12301 aacagcctga ttctgtatct tccgtgctaa aagtttttgg cattttgcag gcgctgggtg
    12361 aagagcgcga aatagggata accgagctgt cgcagcgcgt catgatgtca aaaagcaccg
    12421 tttatcgctt tttacagacc atgaaaacct taggttatgt ggcgcaggaa ggggagtcgg
    12481 agaaatattc cctgaccctg aaattgtttg aactgggcgc tcgcgcgtta caaaacgtcg
    12541 atttaattcg tagcgcagat atccagatgc gtgagatctc ccgcctgacc aaagaaacta
    12601 tccacctcgg cgcactggac gaagacagta ttgtttacat tcacaaaatt gactctatgt
    12661 acaatttgcg catgtattca cggattgggc gtcgtaatcc gctgtacagc accgcgattg
    12721 gtaaggtact gctggcatgg cgcgatctcg atgaagtgaa gcaaattctt gagggcgtgg
    12781 agtataaacg cagtaccgag cggaccatca ccagtacaga agcgttatta cccgttctgg
    12841 accaggtgcg cgagcagggg tatggcgaag ataacgaaga gcaggaagaa gggctgcgat
    12901 gcattgcggt accggtattt gatcgctttg gcgtggtcat tgccggtttg agcatctcct
    12961 tcccgacgtt gcgtttctct gaagagcgtt tacaggaata tgtcgcaatg ttgcataccg
    13021 cagcgcgcaa aatttctgcc caaatgggtt atcacgacta tccgttctga tgagagtaag
    13081 aacctgtcgg aatatcaaac agacaggttc tttatttagc atgagaaaaa taaagttgaa
    13141 ggtgcgttat attaaacgcg cttgctataa gaatatttta ctcatgagtg agaatctggt
    13201 tatttattac ccttaaccat tatcgaccac gatattgctt ttacgtaaca gtgggcaatc
    13261 tgttatcccc aagaaaccac ttttagtgtg caagtattgt accgtgctgg tgcctctggc
    13321 agtcagatag gtacattgca aacctaatcc tgcggcattc tctttgcttc caatcaaaac
    13381 gccatatccg ctgagtaata atcctatcca taccagtgct atcagcataa ctgtgcgaat
    13441 gatgaatcgc attacaacct cttctctttt tatgttcgct taatcgtagc ggcaatatgc
    13501 gctgaagcaa gctactcatt ccgaaaaagc acgaatatcg acatagttag gcgctgttta
    13561 actaacgcat gctagtttaa tgacataagg taggtgaagc ggagattgga gtgaaaaagt
    13621 ttcgatgggt cgttctggtt gtcgtggtgt tggcttgctt gctgctttgg gcgcaggtat
    13681 tcaacatgat gtgcgatcag gatgtacaat ttttcagcgg aatttgtgcc attaaccagt
    13741 ttatcccgtg gtgatatcat tttttcagca ctagattttc ttcctctcca gcagtggtag
    13801 aatagcgcct ctactcagag gtggtgaaat gaacgaagtt gtaaattcag gcgtgatgaa
    13861 cattgcgtct ttggttgtat cggtggtggt tcttcttatc gggctcatct tgtggttttt
    13921 tatcaatcgt gccagttctc ggactaacga acagattgaa ctgcttgagg cgttgctgga
    13981 tcagcaaaaa cgtcaaaatg cactgttacg tcgtttgtgc gaagcaaacg aacctgagaa
    14041 agcagataaa aagaccgttg agagtcaaaa atcggttgaa gacgaagata ttattcgtct
    14101 ggtcgccgaa cgataatcct tcctcaatct tgagtaaccc cgtcgttgaa gggttactca
    14161 attcatcctg attatacgca ctgccatggt gcaactgctt caaattaaaa ctctggtctg
    14221 acttcaaata attatcatat tatttatttg cgtaacggga tatcagaaaa agatattttc
    14281 ctcattaatt tattaggatg tttacatcgg atttgtgatt aagcgtggta ttatttatta
    14341 gcgaaacgtt tctctcttga ttttttgctc attcatcaat ttttcttatt ttaaattttc
    14401 aatccctttg gggattgact tctctttagg gtaattgata gccgttaact gactgtttta
    14461 tgagaaaaag tgatataact ttttattcat tgcatagcaa aaaatgtgat attgcacgca
    14521 ctatgtaata acttctccca ctggcctgga acaactgaac ttattgaact atgttagaaa
    14581 atacgccagt ttaagtatct gcctgaactg gcaaggttaa gcacaatgat atatcggcgc
    14641 gtattccgtt gcataagtgt gcaaaaaaag tggaagacgt atcgagattt gtgcgtctga
    14701 tcgagacatg tttaaaaatg gcttgccata attaacgttg tatgtgataa cagatttcgg
    14761 gttaaacgag gtacagttct gtttatgtgt ggcattttca gtaaagaagt cctgagtaaa
    14821 cacgttgacg ttgaataccg cttctctgcc gagccttata ttggtgcctc atgcagtaat
    14881 gtgtcagttt tatctatgtt atgcctgcgg gcgaagaaaa caatctaagg aatttttcaa
    14941 atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt ctaaaggttt tggcttcatt
    15001 actccggctg atggcagcaa agatgtgttc gtacacttct ccgctatcca gggtaatggc
    15061 ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa ttcaggacgg ccagaaaggt
    15121 ccggcagctg ttaacgtaac agctatctga tcgaatccac tgatctgaag tgtgaatacg
    15181 cttcaatctc gctataaagc ctcgtcgaat gcgaggcttt ttactatgct ttatcttcgc
    15241 tcctggcgtt cggatatttg cccgccgcgt gattcgcgtt acacttgcgg cctttagtat
    15301 cctgccggag ttgtcatgtc gttttcctgt ccactttgcc atcagcctct ttcgcgtgaa
    15361 aaaaacagct atatctgtcc ccagcgacat cagtttgata tggcgaaaga agggtatgtc
    15421 aatctgctgc ccgttcagca taaacggtct cgtgatccgg gcgacagcgc ggaaatgata
    15481 caagcacgcc gcgcattctt agatgccgga cattatcagc cgctgcgtga tgcaattgtc
    15541 gcccaactgc gggaacggct tgatgaaaag gccacggcgg tgctggatat tggctgtggt
    15601 gaagggtatt acacacacgc atttgccgat gtgttgcccg aaatcaccac gtttggcctg
    15661 gatgtctcga aggtagcgat aaaagcggcg gcgaaacgct atccgcaggt cactctttgt
    15721 gtcgcttcca gccaccgttt gccgttttcc gataccagta tggacgccat aatacgtatt
    15781 tacgcgccgt gtaaagcaga agaattagca cgagtagtga agcccggcgg ctgggtcatt
    15841 actgccacgc cgggaccgcg acatttgatg gagctgaagg ggctgattta caatgaagta
    15901 catcttcatg cacctcatgc agaacaactg gaaggtttta cattacagca gagtgatgag
    15961 ttgtgttatc cgatgcgtct tcgcggtgat gaagccgtcg cattattgca gatgacgccg
    16021 tttgcctggc gtgcgaagcc agaagtctgg caaacactgg cagcaaaaga agtgttcgac
    16081 tgccagacgg actttaatat tcacctctgg cagcgttctt attaaccgtg gaagtgcgtc
    16141 cagaggatct ggacgccgat gccgatcagc accagcccgc cgagaatttc cgcttttttc
    16201 ccaataattg agccgataaa gcgaccaacc atcatcccta atgtcgacat aatcaaggtt
    16261 gcacaaccaa tggccaatgc ggtcgcgata atgttgacct gcaggaaagc aagaccaaca
    16321 cccacagcca tggcatccag gctggtggca atcgcggtgg ttaccagtag ccagaaaccg
    16381 tgccgacggc gcggctcttc atcttcatca tctgcgccac gaaaaccctc aataatcatt
    16441 cgcccgccga ggaatatcag cagcacaaac gcaatccagt ggttccattc aaggacaaac
    16501 cggctggcta acatgcccat tccccagccg atcagcggcg tcagggtttc gacggcacca
    16561 aaaataaggc cggttcgcaa tgcttcagaa aatttcggtt tatggagggt ggcaccttta
    16621 ccgattgatg cagcaaatgc atccatcgac ataccaaacg caagaagaac agtagcagtg
    16681 atattcatga caatgtcctg accggggtat ccataaaaca catcatcgcc cccagcaaac
    16741 atgcatacat gaacaacaag ccgcgatggt tttcgcgttc atgcaggcat agcgattgat
    16801 gtgcatatgg tctcgcctga ctgaaagtat tcagtccgta cgtgccacgt tttgcaacga
    16861 gtatgttgac acgtacattt ccggaatctg gaaatcggct actccccaaa tgagggcgca
    16921 accttaac
//
LOCUS       DAZKGS010000097        16682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKGS010000097 DAZKGS010000000
VERSION     DAZKGS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..307
                     /gene="cybC"
                     /locus_tag="ACHOQY_003144"
     CDS             <1..307
                     /gene="cybC"
                     /locus_tag="ACHOQY_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9679197.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHOQY_003145"
     CDS             complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHOQY_003145"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9679198.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHOQY_003146"
     CDS             complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHOQY_003146"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9679199.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHOQY_003147"
     CDS             complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHOQY_003147"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9679200.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHOQY_003148"
     CDS             complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHOQY_003148"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9679201.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHOQY_003149"
                     /pseudo
     CDS             complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHOQY_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHOQY_003150"
     CDS             7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHOQY_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9679202.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHOQY_003151"
     CDS             8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHOQY_003151"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9679203.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10981..11367)
                     /gene="ridA"
                     /locus_tag="ACHOQY_003152"
     CDS             complement(10981..11367)
                     /gene="ridA"
                     /locus_tag="ACHOQY_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9679204.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(11440..11901)
                     /gene="pyrI"
                     /locus_tag="ACHOQY_003153"
     CDS             complement(11440..11901)
                     /gene="pyrI"
                     /locus_tag="ACHOQY_003153"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9679205.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(11914..12849)
                     /gene="pyrB"
                     /locus_tag="ACHOQY_003154"
     CDS             complement(11914..12849)
                     /gene="pyrB"
                     /locus_tag="ACHOQY_003154"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9679206.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(12853..12987)
                     /gene="pyrL"
                     /locus_tag="ACHOQY_003155"
     CDS             complement(12853..12987)
                     /gene="pyrL"
                     /locus_tag="ACHOQY_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9679207.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(13268..13663)
                     /gene="yjgH"
                     /locus_tag="ACHOQY_003156"
     CDS             complement(13268..13663)
                     /gene="yjgH"
                     /locus_tag="ACHOQY_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9679208.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(13794..14507)
                     /gene="bdcA"
                     /locus_tag="ACHOQY_003157"
     CDS             complement(13794..14507)
                     /gene="bdcA"
                     /locus_tag="ACHOQY_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9679209.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            14578..15171
                     /gene="bdcR"
                     /locus_tag="ACHOQY_003158"
     CDS             14578..15171
                     /gene="bdcR"
                     /locus_tag="ACHOQY_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9679210.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            15316..15768
                     /gene="tabA"
                     /locus_tag="ACHOQY_003159"
     CDS             15316..15768
                     /gene="tabA"
                     /locus_tag="ACHOQY_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP9679211.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            15891..>16682
                     /locus_tag="ACHOQY_003160"
     CDS             15891..>16682
                     /locus_tag="ACHOQY_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679212.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEI"
BASE COUNT         4051 a         4135 c         4318 g         4178 t
ORIGIN      
        1 caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca
       61 agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc
      121 gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt
      181 cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa
      241 agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta
      301 tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa
      361 atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg
      421 cacaaatttg ccgtcgacca gtacgttaat caaatcaaca acctgcattt gcgcagcgtt
      481 gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg
      541 gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc
      601 ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa taatctggtc
      661 ttccattgct ttagtaaatg tctgaccgga atttacccgc catgtgcttt tgttatagca
      721 accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt
      781 gacgatgtcg acaggatagt actgatgata attcattgtt tcgcctggct tatcagtccg
      841 cgtataggat gcatccggta atcaacgcct gatgcgacgc tgtcgcgtct tatcagacct
      901 acaatcgttg ttgccatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
      961 acgtagagtg aagattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact
     1021 tcttcctgct taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg
     1081 cggcgagtca ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct
     1141 ttgctggtgc actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta
     1201 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     1261 aggttgtgct gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc
     1321 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     1381 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca
     1441 gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa
     1501 ccataacccg tttcttcttt ccactgatcc actgcctggc gcagacgttc aacaatggcg
     1561 atacctttcg cgcgaagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     1621 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     1681 tcagaaacat catcgtcagc attcagacgt acgccacaag caccttccat atagaggatc
     1741 ggggccacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     1801 gccagcacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     1861 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     1921 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     1981 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     2041 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     2101 agaccatcgc gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg
     2161 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     2221 cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt
     2281 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagtttgcg
     2341 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     2401 gcggtaacaa acggcgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     2461 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     2521 ggcggttcaa tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg
     2581 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     2641 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt
     2701 ttagccacga tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg
     2761 gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctggttc
     2821 aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga
     2881 gccagttgtt tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcgttg
     2941 atatccacct ggttgcggcc ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa
     3001 taatcggcat catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc
     3061 tctgatttaa acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct
     3121 ccatattttt aagaacaggt tatccacaga aattgggaaa ggcgttttcc ggttttttcg
     3181 tttcgttttc cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt
     3241 cgtggggagc tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat
     3301 tttaaagtct atatatagtg ctttgcatca aggatgtttt aacttttttt gatgtagctc
     3361 aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg
     3421 aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac
     3481 cggcattacc ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc
     3541 cgtaaattca tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc
     3601 cagttgccgc gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg
     3661 accagcaagg tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc
     3721 agcagatcct ggtaattgcc ccatgtcagg atggcttctt gcttacgcag tgcgattaac
     3781 ttttggtagg tgtaaaacac cgaggagtca tcggccagcg cggcttctac gttgatttgt
     3841 tgatagttat cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg
     3901 ttgctccatt gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat
     3961 aactcgtcgg catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg
     4021 tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccttgg
     4081 tagatatacg gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc
     4141 acgcggtatt caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac
     4201 cagaacaagg cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt
     4261 ttcaacgcca caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc
     4321 ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc
     4381 tggcaatgct caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta
     4441 aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag
     4501 cgacgcccgt cgccatccag gtcttcaggg aaacgcgggt ctttggagat cagattcacc
     4561 acatccaggc gcaacccgtc gaccccacga tcggcccaga attcacagac ttttttcagc
     4621 tctgcgcgta ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc
     4681 aaatagtact gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc
     4741 cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg
     4801 ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc
     4861 atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg
     4921 tccagcgtgc cgtagatggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg
     4981 acctggggag agatataaaa gggggttagc cagatggcat caacgcccag tttatgcaga
     5041 tagtccaggc gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga
     5101 aaactctttg gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc
     5161 attacgttat tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat
     5221 atccagcgtg cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat
     5281 gatgatggcg ataaccattg ccagcgcaaa tacctgccag tagctcggtt gaatcgagag
     5341 aattcccggc aggccgccta cgccgatacc attcgccata acgccgttca ggccgcatag
     5401 taatcctgcc agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt
     5461 gatgccgtac attgcaggct cagtgacccc aagccaggcg gagatagcgg caggcacgga
     5521 gatctcgcgt tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg
     5581 agcgatattc gacagcgcaa tcagcggcca cactggcgtg ccacccatgc tttgaatcat
     5641 ctgcaaatca atagcaaggg tggtctggtg tacaccggtg atcaccagcg gggcgtacag
     5701 gaagccaaac aatgctgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc
     5761 aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa
     5821 caccgcgagg atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat
     5881 gcgtttaagg cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac
     5941 ctgcgcctga tagcctactt tggcgatgct gaacatgcca aagtcccaca cttccggcag
     6001 ctgctgcccg agcagataag cgttcatcag ctggggagaa accagtgtca cgccaagcac
     6061 gataccaagg atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa taccgaccgg
     6121 caggtagaag aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga
     6181 agggtacatt tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg
     6241 aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc agcgggaaga agatctccgc
     6301 gaaatgagag atcaactgct catgccattt catattctgc cgggcggctt tttttacctg
     6361 ctctttatca acctgcgcct gtccggttga cgcaatcagt gcttgatagt aatcacccac
     6421 attggtgcca atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg
     6481 gagttgctca atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg
     6541 agtaatacag tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg
     6601 atcgatatcc gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg
     6661 gttggctgca gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacgcag
     6721 cgaagatcac aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt
     6781 gttgcggttc gctgcgcccg gttacctgtg cgatcaattg gcaggccgcc tggcgtccag
     6841 cttcggcgta accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg
     6901 tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg
     6961 cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt
     7021 ttgctacgtt ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca
     7081 gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt
     7141 cactgtgcgg cacgccgaga taactgatat tacgatgccc ctggtcatag agccgttgca
     7201 tcaggatctt gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac
     7261 gcgccagcag aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa
     7321 aaccgaacag cactacgccg tcgatattac gccgttttag gatccccaaa tgttcggcaa
     7381 ctaattgcgg ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg
     7441 ctggcagcat ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga
     7501 tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc
     7561 catgctgatt catcactgct tccacgcgct cgcgcgtgcg ctggctcacg ccgctttcgt
     7621 tattcagcac ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga
     7681 tggtcagccg attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgaggctaa
     7741 ctttacccgt gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca
     7801 ggtattatat cgccataaat gctacgaata ttattggatt ctccttatta tttgcggcgc
     7861 ttttttcact taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat
     7921 taaaactttt tcggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg
     7981 ttttaacgtc cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca
     8041 tttctgtact gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg
     8101 gctcattcgc catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca
     8161 gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat
     8221 gcccgaagaa gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga
     8281 agtggaatct gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc
     8341 gtggtgggta catttatggg tctgctatcg caaccccttt aatattttac tcaccattct
     8401 cggcgctatt tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt
     8461 cgccatttct acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc
     8521 cctgaaagcg atggtcagca atactgcgac ggtgctgcgc gtaattaacg acaaaggcga
     8581 aaatggctgg ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc
     8641 ggcgggggat atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc
     8701 tcaggcgtcg ttaaccggtg agtctctgcc cgtagaaaaa gcagctacca ctcgccagcc
     8761 ggagcacagc aatccgctgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag
     8821 cggcacggca caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc
     8881 ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt
     8941 cagtatgctg ctgattcgct ttatgctggt gatggctccg gtggtgctgt taatcaatgg
     9001 ttacaccaaa ggcgactggt gggaagcggc actgtttgcg ctttcggtcg cggtaggctt
     9061 aacgccggaa atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct
     9121 gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga
     9181 tattctgtgc actgataaaa ctggcaccct gacacaggac aaaattgtgc tggagaatca
     9241 taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca
     9301 ttatcagacc ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga
     9361 gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga
     9421 gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa
     9481 aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt
     9541 gccgctcgat gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca
     9601 ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca
     9661 gcgggcggat gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa
     9721 agagacaact gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct
     9781 tactggcgac agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg
     9841 agaggtggtc attggtagtg atattgaaac gctatctgac gacgaactgg caaatctcgc
     9901 acagcgcacc acgctgtttg ctcgtctgac gccgatgcat aaagaacgca ttgtgacctt
     9961 actgaagcgc gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc
    10021 cttacgcgct gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc
    10081 ggctgatatc atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg
    10141 acgtcgcact ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg
    10201 taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt
    10261 acacttgctt attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa
    10321 cgtcgacgac gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt
    10381 tatgatcttc ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg
    10441 gtgggtattc catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt
    10501 ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt
    10561 tattcagagc tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg
    10621 gatcgcattg ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag
    10681 ctatttcccg tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa
    10741 agggttctat agccgtcgtt acggctggca gtaaagaata aactgggcac gatagccccg
    10801 attcagattg cagacaaagt gagcgttgtt catgccggat gcggcgtaaa tgccttatcc
    10861 ggcctacaaa ttcaatcaat tgcaggaatc acgtagatac tgataagcgt agcgcatcag
    10921 gcaattttgc gtttgtcatc agatttaatc cgggcatgat agcccggatt tccatcaaga
    10981 ttagcgacga acagcgatcg cttcgatctc aatttttacg tctttcggca gacgggcaac
    11041 ttcaacgcaa gaacgtgccg ggaaggtggc attgtgttcg gtgaagaagg cttcgtaggt
    11101 agcattaacg gttgcgaagt cgttcagatc tttcacaaac acggtggttt taacgatgtc
    11161 gcccactttc aggcctgcgg cttcgacgat cgcttttacg ttatccagcg actgacgtgc
    11221 ctgtgcagcg acgtctgccg gtacttcgcc cgttttcgga tttaccggga tctggccgga
    11281 ggtgatgatc atattgccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc
    11341 attttccgtc gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg
    11401 gctcattata gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt
    11461 atgggaaaac tctttttcac agtatttgca tttgagcgcg atgtcattgg cgcgttttcg
    11521 cacggcaaag ctggaagaaa ccggttcggc atggctgata cagttgctgt tcgggcagac
    11581 cagcacattg tcgatgcgct ccggcagact ggggcgcgat ttacccacca cttcatagtt
    11641 gtcgatacgg ttaaccgtgg cttgcggcgc atacaatgcc agttgatcta cctgatcttc
    11701 actcaaaaag gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcag
    11761 gttcagacca atggtgatgc gctgatccgt ttcggtcagc ttgaacagac tcaacagctt
    11821 aaaaccgatc tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg
    11881 caatttatta tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac
    11941 cagtgccagt aacgcctggc gagcgaaaat cccgttgcct gcctgctgga agtaccaggc
    12001 gtgtggcgtt ttatcaacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac
    12061 tttcatattg gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac
    12121 gttggcgtac tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc
    12181 tacttccgcc atcacttctt caatagagct gtgcagactc catgcgatcc ctttttcatc
    12241 gagcatatcc agaatgtatt gcggcattgc cagcgcgtcc ggcgcgatga agtaaaaacg
    12301 gttgccatcg aacttcgcta acgcctgagt cagggagtga acggtgcggc catatttcag
    12361 gtcaccaacc attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatagtgaa
    12421 taagtccagc aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg
    12481 tacattgccg gaaaactcgg tggccaggcg cgccgcacct tcctgcggat gacgcatcac
    12541 tatcgcatcg acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcccttttt
    12601 acccagtgat gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat
    12661 agatgtttcg aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac
    12721 tttgtgcttc aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac
    12781 cagattaagg tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg
    12841 attagccatc ttttatctcc tgacgcctgg gcaaaaaaaa gcccctcgat tgaggggctg
    12901 ggaatgggtg atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa
    12961 gacaaaatgt cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact
    13021 catagccaga agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt
    13081 gaattaaaag tcaaaatgta tgcattttta tttgctttga ttctggagga gaactgtcgt
    13141 tcggtatcaa taattgcaga catttcctgc ttttttcttt ttcactttca cgcaatcaga
    13201 tatgcatttt attccattca tccgacttat agggcggagt gtgaaaacga acggctaaca
    13261 ctattgctta cagctcaggg atgcgcgcta tcactttaat ttcaaaatca aagcctgcca
    13321 gccaggtaac gcccaccgcc gtccagtttg gataaggagg ggcgctaaat atttcatttt
    13381 tgaccgtcat gatgtcttca aattgttttt ccggatcggt atgaaagctc gtaacatcaa
    13441 tgatatcgtc aaaagtgcat cccgcagcag ccagggtcgc atgcaaatta tcaaatgcca
    13501 gtctgacttg ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc
    13561 cggaaacaaa cagcaaatcg ccggaacgaa tagccgcaga ataacgatgc tcagcataca
    13621 gtgaatgtcg gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat
    13681 ttacgacatg aacagaagat ttctctttac cgggagccgc ttttagcgga cgacgtgagt
    13741 aaacaaaacc cagacatcat ggataatggc tgggcttaat tgagcgtagt cggttatgcg
    13801 ccaaacgcgc catcaatggt atgcatcgcg ccggtaacaa aactggcttc tggccctgct
    13861 aaccatgcga ccataccagc gacctcttcc ggttgcccat gtcttttgat agccatcaaa
    13921 ctatgcaaca tatcgcgcat tggcccgttg gcgggattag cgtcggtatc aattggccct
    13981 ggctggacga cgttaatggt gatcccacgc ggtccaaaat cacgggccag cccgcgcgcc
    14041 atgccttgca gggcagattt gctggcggca taagcagcca tgcctgcaac aggcatacga
    14101 tcgccattca cggagccgat gattaagatg cgcccgcctt cgggcatctg ccgggcggct
    14161 tcaacagagg catgataagg agcatgaata ttgattttga aaaggcgatc aatatcgtcg
    14221 gcatttaatt ccagggcatc gccaaagacg ccaatacctg catttaccac caggatatcc
    14281 aatgcgccgc tcttacgaac gacatcaatg acagcgtctc tgtcagcact atctgtaaat
    14341 actgctgtcg ctccagtctc ttgtgccaga tgttcagcgg catctttcga ccccgcatag
    14401 gtgaatcgta cattggcccc atcagtgacg aaacgacgta cgatagcggc accgattcca
    14461 cgactgccac cgaggatgag aactgtctta cctgtaaaag cgcccataag gactccttga
    14521 tttattatgt aacatgcatt acaaaactgt tttaactttc tgtcaacaag ttttgtaatg
    14581 gtcactaaaa aacaatctcg cgttccaggt cgtcccagac gtttcgctcc tgagcaggca
    14641 gtctctgcgg caaaagtgct ttttcaccaa aaaggtttcg atgctgtcag tgttgctgaa
    14701 gttactgatt atcttggtat taaccccccg agcctctacg cagcttttgg cagtaaagtt
    14761 gggttattta gccgtgtact caatgaatat gtcggtacgg aagctattcc gcttgccgat
    14821 attcttcgtg atgatcgtcc ggtaggcgag tgcctggttg aggtattaaa agaagcggcg
    14881 cgcagatata gccaaaacgg cggctgcgct ggctgtatgg ttcttgaagg tattcatagt
    14941 catgatccac aagcgcgtga tattgccgtt caatattatc acgccgcaga aacgaccatt
    15001 tatgactata tcgccaggcg gcatccacaa agcgcacaat gtgtgactga ttttatgagt
    15061 accgtgatgt cagggctttc tgcgaaggca cgggaggggc actccatcga acaactctgt
    15121 gcaacagctg cactggcggg ggaagcgata aaaactattc tcaaggagtg atgctggcct
    15181 tgacccgaaa ggcgggaacg cgcctgccga taagttgtga taagacaata attcacgcgt
    15241 taaggctagc ggaattgatc atcttttcgt ataacggtgg aaatgaaaca ttgttttaat
    15301 taaggagtgg aaaagatgat catcggaaat attcataatc ttcagccgtg gctaccccag
    15361 gagttacgcc aggcgattga gcatatcaaa gcgcacgtta cggcagaaac gccaaagggt
    15421 aagcacgata tcgaaggcaa tcgtctgttt tatcttatct cggaagatat gaccgagccg
    15481 tacgaagctc gccgtgcgga gtatcatgcc cgctatctcg acattcagat tgtgttaaaa
    15541 ggtcaggaag gcatgacctt cagcacgcaa cctgcaggca cgccggatac cgactggtta
    15601 gctgataaag acatcgcatt tttgccggaa ggcgttgatg agaaaacagt tatccttaat
    15661 gaaggtgatt ttgttgtgtt ttatccgggg gaagtgcata aaccgctgtg cgcagtgggc
    15721 gcgccagccc aggttcgcaa agcagtagtg aagatgctga tggcgtgatg acttttcgcc
    15781 gtaaataact ccaggtttac ggcgagtttg tgaaaagagc gttttttgat atttttttgt
    15841 gagtagaatt tgtaatgctt agacgttctt attaactcaa ggagtccgtc atggcaaaaa
    15901 tatcagattt gaattgttat caacacatgg catcagccga caatgttaaa ccaaaaaatg
    15961 aagtcttaaa tacctggtgt gtgggaatga atgattttgc tcgtaatgcc gaggggcagg
    16021 atagcagaag gaatattcta tctcctggag catttttgga gtttttggca aaaatattta
    16081 ccctgggtta tgtggatttt agcaaacgct ccaacgaagc gggtagaaat atgatggctc
    16141 atattgagtc ctcatcttat atcaaaaata atgatggcag tgaaataatg aattttgtta
    16201 tgaataatcc tgaaggtgag cgtgcggatt catctaaggt gattatagaa atttcacttt
    16261 ctactaccac tactatgggg actcgtcaag gacatacagc cattatattt ccacaatctg
    16321 attttttgag ttttcgttat gaaggtaagt cttttgaaag aaaagatgat agttcattac
    16381 atctgattac taacaaggtt ctggcgtgtt accaacgcga agctaacaag aatatggcgc
    16441 ttctattaaa taatcatcag ttgaactgga aggacgcaat tgattataac gagttattag
    16501 aaaagccatt agccagtaca ttagaaaaaa taaagaaaga acatctgcta ttaatgcctc
    16561 atgtatgtga tgacacgatt ccatacttac taggcgaagg tggtatactt gaagaaataa
    16621 atgatctgaa agcgttacat gataccagaa ttgctagtgg tacagaaggt aatgatgaaa
    16681 tt
//
LOCUS       DAZKGS010000098        16648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKGS010000098 DAZKGS010000000
VERSION     DAZKGS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..224)
                     /locus_tag="ACHOQY_003161"
     CDS             complement(72..224)
                     /locus_tag="ACHOQY_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9679213.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(489..1223)
                     /gene="cysH"
                     /locus_tag="ACHOQY_003162"
     CDS             complement(489..1223)
                     /gene="cysH"
                     /locus_tag="ACHOQY_003162"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9679214.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1297..3009)
                     /gene="cysI"
                     /locus_tag="ACHOQY_003163"
     CDS             complement(1297..3009)
                     /gene="cysI"
                     /locus_tag="ACHOQY_003163"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9679215.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3009..4808)
                     /gene="cysJ"
                     /locus_tag="ACHOQY_003164"
     CDS             complement(3009..4808)
                     /gene="cysJ"
                     /locus_tag="ACHOQY_003164"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9679216.1"
                     /translation="MTTQVPLSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAAGEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5127..5489
                     /gene="queD"
                     /locus_tag="ACHOQY_003165"
     CDS             5127..5489
                     /gene="queD"
                     /locus_tag="ACHOQY_003165"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9679217.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5567..6838
                     /gene="ygcN"
                     /locus_tag="ACHOQY_003166"
     CDS             5567..6838
                     /gene="ygcN"
                     /locus_tag="ACHOQY_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9679218.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6829..7089
                     /gene="ygcO"
                     /locus_tag="ACHOQY_003167"
     CDS             6829..7089
                     /gene="ygcO"
                     /locus_tag="ACHOQY_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9679219.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7106..7681
                     /gene="ygcP"
                     /locus_tag="ACHOQY_003168"
     CDS             7106..7681
                     /gene="ygcP"
                     /locus_tag="ACHOQY_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9679220.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7829..8689)
                     /gene="ygcQ"
                     /locus_tag="ACHOQY_003169"
     CDS             complement(7829..8689)
                     /gene="ygcQ"
                     /locus_tag="ACHOQY_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9679221.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8686..9465)
                     /gene="ygcR"
                     /locus_tag="ACHOQY_003170"
     CDS             complement(8686..9465)
                     /gene="ygcR"
                     /locus_tag="ACHOQY_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9679222.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9443..10852)
                     /gene="ygcS"
                     /locus_tag="ACHOQY_003171"
     CDS             complement(9443..10852)
                     /gene="ygcS"
                     /locus_tag="ACHOQY_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679223.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10874..12328)
                     /gene="ygcU"
                     /locus_tag="ACHOQY_003172"
     CDS             complement(10874..12328)
                     /gene="ygcU"
                     /locus_tag="ACHOQY_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9679224.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12398..13182)
                     /gene="ygcW"
                     /locus_tag="ACHOQY_003173"
                     /pseudo
     CDS             complement(12398..13182)
                     /gene="ygcW"
                     /locus_tag="ACHOQY_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13500..14777
                     /gene="yqcE"
                     /locus_tag="ACHOQY_003174"
     CDS             13500..14777
                     /gene="yqcE"
                     /locus_tag="ACHOQY_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9679225.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14804..16282
                     /gene="ygcE"
                     /locus_tag="ACHOQY_003175"
     CDS             14804..16282
                     /gene="ygcE"
                     /locus_tag="ACHOQY_003175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9679226.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3970 a         4368 c         4187 g         4123 t
ORIGIN      
        1 aattttaaaa aagggtgcag gagaccaaac catcacaatt cagtacgcgg ggttttaccc
       61 ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc cataccacgt
      121 tctctgatac tcaactcaca caacgagtca cctaccatca gcgtgaatcc cagtactgta
      181 caacacagta cgatgattgc cacaagggca tattttgtca gcatgtcttg cctccctgca
      241 aagaagagac taacattcga attgtttggg ttcgaacgta agcctcaggt tgatagaaat
      301 atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc ttaacgccag acagcctcaa
      361 gcacccgacg ccattctata cctgataatt cttccagcgg cgttcacgtt taaaatcagg
      421 aatactgctc gcaaaataaa aacgccccca tcatttctga cagaggcgtt taatttatcc
      481 ggcaatattt acccttcgtg caatccacat tcccttttta agccaaagaa acgtgtttct
      541 tcttccgaca tgccaggttc ccatttacgg gttgtatggg tatcgcccac cgataaatat
      601 ccttcgtccc ataatgggtg atatttcagg ccatgttttt gcagatactg atatatcgtt
      661 cggttatccc agtcgataat cggcagcact ttaaatacgc cacgctgaat tgccagcacc
      721 ggtaaattgg cgcggctgcc ggattgttcg cggcgcaggc cagcaaacca cgtttgcgca
      781 ttcagttctt tcagcgcccg gttcatcggt tcgactttgt tgatgtcatt gtacttttca
      841 atgccttcaa cgccctgttc ccacagtttg ccgtagcgtg cttcctgcca ggcggcgctt
      901 tcggtagcgc ggtacacttt caggttgagc ttgagtttgt ccgttaactc gtcaataaag
      961 cggtaggttt ccgggaacag atagccggtg tcggtgagga tcaccggaat atccgggtga
     1021 atctgattca ccagatgcag gctcaccgcc gcctgaatgc caaagctgga agaaagtaca
     1081 tattcaccgg gcagattatc cagcgcccag gcaacgcggc cttcagcgtc cagtttttcc
     1141 agttcggcgt tagtttctgc cagcgccaga atgcgatcta ccttcggcag ttcgttcagg
     1201 gcgtttagat cgagtttgga catagattcc tcactgtttg ccttgcctga tgcgacgctc
     1261 acgcgtctta tcaggcctac aagaccgggc tgatggttaa tcccacaaat cgcgcgccgg
     1321 atcgagcacc gggcgaatga tgcccgcacg caccgtaaag tcgccgaagc cttcacccgc
     1381 ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc gacgccagga tttccggctc
     1441 ggtgatgttt tctttataca tccgtgggat acgtgtccca atgcggttgc cgccaagatg
     1501 caggttgtag cgacccggcg ctttacccac caggcccact tccgccagca tcgcgcgacc
     1561 acaaccgttc gggcagcctg ttacacgcat cacgatatgc tcatcgctga caccatgttt
     1621 cgccattaaa ttatcgatgt tgtcgataaa agacggcagg aaacgctctg cttccgccat
     1681 cgccagcggg caagtcggga acgagacgca ggccatcgag ttttcacgct gcggcgtgac
     1741 ggcattcatt aacccgctct ctttggcgat cttctcgatc ttcgctttct cgctttccgg
     1801 tacaccggcg atgatcagat tctggttcgc cgtaatgcgg aaatcgcctt tgtggatctt
     1861 cgcgatctcc agcaggccgg ttttcagcgg acgccccgga taatcaagga tgcgaccatt
     1921 ttcgataaac agcgtcaggt gccagttatc atcaatgccc ttaacccagc caatacgatc
     1981 gcctcgtccg gtgaactcat atggacggat cggttcaaat ttgatccccg cgcgacgctc
     2041 cacttccgct ttaaacgtct caacccccac gcgctccagc gtgtatttgg ttttggcatt
     2101 tttacgatcg gttcggttac cccagtcacg ctgagttgtc acgacggctt cggccaccgc
     2161 cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc gtgcgggcgt aggttttctt
     2221 gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg ttgaagccta ccagcttgcc
     2281 gttttcggcg attgccacga agttcatgtc gttggcatgc agatcgatat cgttctgcgg
     2341 cgggatcact accgtggttt tgaacttacg cggcaggtag gtctgaccaa ggatcggttc
     2401 ttcatcagta gtggcgactt tttcgtggtc gagccagatc tccgcatacg cgcgggtacg
     2461 aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac gcttccgcgt gcagctgcga
     2521 ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg tcgttagctg tcgccagcgc
     2581 atcaagaccg accgagtgca gcatctggtg caccggtttg acgttctttt tcagaatgcc
     2641 gtggaactga aacgtctggc ggttggtcag gcgaatgctg ccatagatgg tgttttcacc
     2701 ggcaaatttg tcgatcgcct gccactgttt agtggtaatc accccacccg gcagacgaca
     2761 gcgaagcagc atcgcgtggc gcggctccag cttctgttca gcacgttcgg cgcggatgtc
     2821 gcggtcatcc tgctgataca tgccgtggaa gcggatcagc aggaagttgt cgcccttaaa
     2881 gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg ccgcgcaggt agttgctttc
     2941 aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc actaaaggcc ctggatgttt
     3001 ttcgctcatt agtagacatc tcgctgataa cggcgctcta cgcgcagctc acttaaaaat
     3061 tcatccgccg cttcggtgtc catgccacca aattcggcaa tcacttccag aagtgcctgc
     3121 tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat aaatgtgggc accatcattg
     3181 atccagcgcc acagctccgc gccctgttcg cgcagtttgt cttgtacgta aactttttct
     3241 ttttggtcgc gcgaccaggc aagatcgata cgtgtcagca cgccatcttt gacgtagcgc
     3301 tgccactcca cctgatacag gaagtcttcc gtaaagtgcg gattaccaaa gaacagccag
     3361 tttttacctg gcgcttcgcc ggcggcgcgt tgctgcataa aggcgcggaa cggcgcaata
     3421 ccggtgcctg ggccaatcat aatcaccggg gtttccgggt tagcgggcag gcgaaagtta
     3481 tcgttatgtt cgataaatac gcggacttcg ccctcttctt ccacgcgatc cgcgaggaag
     3541 ctggaggcac caccggcacg ggctcggcct tccacgtcgt aacgcaccac accaacggtg
     3601 acgtgtactt cgttctcgac ttccgcctgc gaggaggcga tggaatacag gcgcggggtc
     3661 agcgggcgca gcagattaat cagcgcttcg gcatccagtt gcgccggaga gaaacgcacc
     3721 atgtcgacaa tcggcgtcgt cgcggcgtaa tgctgtaact tcgctttatc gcccaccagc
     3781 ggcagcagtg tttcgctgcg ggtaagcgtg gcgtaattct caacaatgtt ggcggtgttg
     3841 acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag gcaacgtttt gccctcgacg
     3901 gtgacaggtt catcgccttt cagccacagc agttcgacaa gttctttcac cagtgccgga
     3961 tcgttctgat accagacgcc cagcgcgtca cccggctggt aacgcaggcc cgagtcacct
     4021 aagtcaattt cgatatggcg aacgtctttt tcagagttac gcccggtaat tttctggtta
     4081 acagagaggc tagccaccag cggcgcgtct ttgctgtacg ggctggtgtg gatttcattt
     4141 accgcgccag tagcgacgga ttgcgaaggt gccgcgacag gcgcacgcga tttaagcgca
     4201 tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt attcaacatc ggcatcgaca
     4261 cggtcgagca ggcgttcacc acccagttcc gccagcttgc tgtcgaaatc tttcccggac
     4321 tggcagaaaa attcataaga gctatcgccg aggctaaaca cggcaaacgc ggtgttttcc
     4381 agctttggcg cttttttgga gaacaggaac ttatgcagcg cgacggcttc ttccggcggt
     4441 tccccttccc cttgcgttga cgtcactacg atgagcagtt tttcgctggc gatttgtttg
     4501 aatttatagt cgcccgcgtt caccagctta acgttcagtt ttgctgctaa taaatcatcg
     4561 cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg aggcggagat aatagttata
     4621 cccggcattt ctgcggctgg cgctggcgtc gctgcaagcg cagcaggctg ctgattgagc
     4681 acgccccaga aatagccaga aacccaggca agctgggtgg gagttaaatc ggtcgtggcc
     4741 gcctgaaggc gtgccagttg ctccgggttc aacggaagca acgcggaaag tgggacctgt
     4801 gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt ctgcgccctg tcagcgccca
     4861 taaaacagaa gagaaggtaa ggttaacggg gtgaacggtg tggattaaag acgggatagc
     4921 gataactaat aaccaaatcg actaacctgt tttagcaata gtctttaaca acaaaataga
     4981 ttaaccaacc taatgaaaaa caaatgaatt tagccaatca ttaagataaa tcagcgattt
     5041 tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc tttccggtac tctacggcgg
     5101 ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt atttaaagat ttcaccttcg
     5161 aagccgctca ccgcttacca cacgtcccgg aagggcataa atgtggtcgc ctgcacgggc
     5221 attcctttat ggtgcgactg gaaattaccg gggaagtcga tccgcatacg ggctggatta
     5281 tcgatttcgc tgaactaaaa gcggcgttta aaccaaccta cgagcgcctc gatcaccatt
     5341 atctcaatga tattccaggt ctggaaaacc caaccagcga ggttttagca aaatggattt
     5401 gggatcaggt taaacccgtt gtgccgctgt taagtgcggt gatggtaaaa gaaacctgta
     5461 ccgcaggttg tatctatcgc ggcgaatgat aagagtgtgt cggcggtcaa tttcccttaa
     5521 gtaacgctat gttagggtgt tgtgttctgg atatctgggg catgacatgg aagacgactg
     5581 cgacattatt attattggtg ccggtattgc aggcaccgct tgcgcgttac gctgcgcgcg
     5641 agcgggttta tccgttttgt tactggaacg cgctgaaatc cccggcagca aaaatctttc
     5701 cggcgggcgg ttatataccc atgcactcgc ggaactcctc ccgcaatttc atctgaccgc
     5761 gcctcttgaa cgacgcatca ctcacgaaag cctttccctg ttaacgccgg atggcgcaac
     5821 gacgttttcc agcttacagc ccggcggtga atcctggagt gtattacgtg cacggttcga
     5881 tccgtggctg gttgccgaag ccgaagaaga aggtgtcgaa tgcatccccg gagcgacggt
     5941 ggatgcactg tatgaagaaa acggcagggt gtgtggtgtc atttgtggtg acgatattct
     6001 ccgtgcccgt tatgtggtgc tggcagaagg tgccaacagc gtcctggctg agcgtcacgg
     6061 gttagtgact cgtcctgctg gtgaagcgat ggcgttgggg atcaaagaag tgctgtcgct
     6121 ggaaccatcc gctattgaag aacgttttca tctggagaat aacgaaggcg cagcgttgct
     6181 gttcagcggc aggatctgtg atgacttacc cggcggcgca tttctttata ctaatcaaca
     6241 aacgctctcg ttagggattg tttgcccgct ctcttccctt acgcaaagtc gtgttccggc
     6301 aagcgagctg ctgactcgct ttaaagcgca tccggcagtg cgcccgctta tcaaaaacac
     6361 ggaatcactg gagtatggtg cgcatctggt gccagaaggt ggcttgcaca gtatgccggt
     6421 gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg ttgcgcagtt gcgtcaatac
     6481 cggaatttcc gtgcgcggca tggatatggc gctgactggc gcgcaggcgg cggcacaaac
     6541 gctgataagc gcctgccagc accgcgagcc gcaaaatctg tttgcgcttt atcatcacaa
     6601 cgtagagcgc agcctgctat gggatgttct acaacgttat cagcatgttc cggcgctttt
     6661 gcaacgccct ggctggtatc gggcgtggcc tgcgttaatg caggatattt cccgcgattt
     6721 atgggatcag ggtgataaac ctgttccacc gctgcgccag ttattctggc gtcatttacg
     6781 tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg agtctgcgat gtctgtagcc
     6841 cataatctct ggcgcgttgc tgatgcgccg cacattgttc cggctgactc cgttgagcgt
     6901 cagatggtac aacggttgat taacgcctgt ccggcaggtc ttttttcgct cacaccggaa
     6961 ggtgacttac gcgttgatta tcacggctgc ctggagtgtg gcacctgctg tttgctgtgc
     7021 gacgaatcca cactacaaca gtggcgctat ccgccttccg gattcggcat cacctaccgc
     7081 tttggataaa taataaggat aatttatgcc cctcttacac ctgctccgcc agaatccggt
     7141 gattgctgcc gttaaagata atgccagcct gcaactggca atcgactctg aatgccagtt
     7201 tatttccgtg ttgtacggca atatctgcac catcagtaat atcgttaaaa agattaagaa
     7261 cgccgggaaa tatgctttta ttcatgttga tttgctggaa ggcgcgtcaa ataaagaagt
     7321 cgtgattcag tttttgaaac tggtgaccga ggcggacggc attatcagta ccaaagcctc
     7381 aatgttgaaa gcggcaagag cggaaggttt tttctgtatt catcgcctgt ttattgttga
     7441 ttcaatttcg tttcacaaca ttgataagca agttgcgcaa tcgaatccgg attgtattga
     7501 gatcctgcca ggctgtatgc ccaaagtgct gggctgggtg acagagaaaa tccgccaacc
     7561 gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg cgtaatgcga ttaacgcggg
     7621 tgtcgtggcg ctttccacca cgaataccgg ggtctggacg ttagcgaaaa aattactttg
     7681 acgggataat cgtaaccaat tgaatttggt ttgatttgta ggccgcacgc cacatccgac
     7741 attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaagtcc ccaggccaga
     7801 accgtaggca ggatcatccg gcaacacgtt attggcaatc tgcgtgaatg tttgtcacca
     7861 gtgcctccag caccactttc cagtcatcaa ccacgccaac atctgcctgt gaaaataccg
     7921 cagcgctggc gtcatgatta atcgccacca caaacttgct attgcgtacg cccgccatta
     7981 acgctgctgc gccagaagcg ccaacaacaa tacacacctc aggtgccagc agatgtccgg
     8041 aaataccaat cactttttca gcatcaacgc caccgttcat cactcgcgcc cgactgtagc
     8101 ccacttcggc tcccagcttt tctgccagca tagcgatttc ctggttatct gcctcaccgc
     8161 cctgccccac caccagcacc cgtctggcct cagctaatgg gtcacgggta acatttttca
     8221 gatcctctgt gctaaccagc cagtcgggaa gcgcgccggg gacaatgttc agttgctgca
     8281 taccagaggg caacgtggcg tttttcactg ctcccgcctg acgcgccagg gaaaggcata
     8341 gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt tccccagtgg gatttgcgca
     8401 cgcttacggt tggaatatct aacgaagtca cctggcagat gcttgcacca tgtaaacgcc
     8461 aggccagccg ggtcgacagt tcatcgccaa acgttcccgg cggaaagaga acaacgtccg
     8521 caggcgttcg ctgccactgt tccaccaacg catccagcac ctgttccgcc actacaggct
     8581 gaggttcaat ctgccagtgc gccagggtac aaccgctgaa atcctgcgcc gccagccagc
     8641 ttgctattgc ggcattctcc tgatttattg tcacaattgc gatattcatg gctgcatcct
     8701 ttggcgtaaa tagtcctgcc atagcttttg agctttttct gctaccgttt gcccatcaat
     8761 cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc agacactgca tagctggcat
     8821 ttcggcagca acagttttac ggataatttc tgctttcccc gcagccattc gctggcgcat
     8881 tcccggtact ggcaaggcca cttcaccgca ctgacgcacg gcaatcaccg caggcaagcg
     8941 aacccggcaa caacgcaaac catgttcaat acgctgttcg agggtgataa acagcgcgtc
     9001 gagagtgaaa cgctccacct gggtgaagca gggccagccc agcatttccg ccagcaaaaa
     9061 tggcgtttgc ccattctgcc cttcgctgct ttggcagccg gtaattatca gatccagcgg
     9121 attctgatgc tgccattcgg caatatggcg agcgacaaat tccggcgcaa agcgcagatc
     9181 tgccgccgtc tccagcaata cagcttcctc aaacccaaga gccatgagat agcgcagcca
     9241 gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa gacatcggcg taccgttttt
     9301 tctctgcgcc aacagcagcg cggcagcggc ctgttcatca gcaccgagta aacttcgcag
     9361 tagcgaaata tccggtccgc ttttaccctg agccgccgcc tgccactctt tttccgccag
     9421 cattccggca tccggttcgg ctttaaacgc taacagaatg ttcattcgca cctcctacat
     9481 ttcccgccgc caccagcggg agggctttag tttctggtgc ccatagccag gtcacaacaa
     9541 aaccaaccaa caacactgtc gccaggagca ataaggtgac ttgcattccc cactgcgcca
     9601 gcacccacgg cagcaggcca gtacttaccg ccgcgccaag tcgactcatg gcggtggcaa
     9661 aaccgacgcc cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa atgcccacca
     9721 gattactgac tgccgaaatg gtggtgctga agagaacaaa aagcagcagc gttaatgaac
     9781 tgccggaagg caaacaggcc attactacca gcgttgccgc cagcagcaaa aaacttccca
     9841 gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa tcccagcagc gcgcccacaa
     9901 ttaacaacgc attaagcatc aggctggcag tcagcgcatc ttccagacca atagtctggg
     9961 cgatagttgg cagccaggta taaatcacaa accatgggat tacgaggcag acaaagaaga
    10021 cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa caaggttttg atgtgtttat
    10081 gggtcgccgt taccacttca tcgcccagta aaacatgggg accaaaatag cgatgcacga
    10141 tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca gcgtggtgat tctggcgttc
    10201 cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc cgatgccagt agccagcgcc
    10261 aggcctccgg gttttcggaa ataaagtgat gtccggcaat acttgccagc acatagccta
    10321 cggtccacac cacgctgaat gcgcccagca aaataccgcg atggcggcgc ggggaaaatt
    10381 cagccagcaa ggtgtgacct actgaataat cgcctcccag accaatgcca atcaaaatgc
    10441 gcagtccaat aagatgctct ggcgtggtgg caaaaaattg taaaaacgaa gcaagcgtaa
    10501 tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat atggtcggag atccacccaa
    10561 gaaccaggct gccaaggaac aaaccaagga gcgccgagcc gccgatcatt cccgccataa
    10621 acggcgtcag ttgcatggcg ggcgtaagct gaataatggc gtaaccaatg acgccgagaa
    10681 cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc aatgcggcag tgaaaacggt
    10741 taagcggtaa atcatccatt cgcaccggtg aagtgttcat ctttacccct tcattgtccg
    10801 gtaataagaa cggcttctcg cctgagaagc cgaaaaggca acgccccgtc atcgcggaac
    10861 gctgcctgac acattatttt tcaatcggat agatagtacc cgtattcata atgccattag
    10921 gatcgaactg ctttttcagc ccttccagca acgcccacgc gctgccgtgt tccagtttgc
    10981 tccagtgaac gcgatgttta ccgataccgt ggtggtgcac catcgaaccg ccgaggcgaa
    11041 tggtttcttc acagatgatc ttgttgagcg gattgtggta cttgtcgatt tcctcttccg
    11101 gcttacagtc aacgacgttg taatcgtaga cgaagtacat gttggtgccg ttctgatagc
    11161 tatgagagga atgaccgccc agcatggtga tgtcgtcggc gtgcgggaac tcagtacgaa
    11221 tacggttaat aacgctttcg tagatttcgt ggatgcagct ccagcagccg gacacttcag
    11281 tggtaaagcc catgttgccg gttttgagga tctgcacacg ttcggcagcc actttatccg
    11341 gtccccagtt caggttgtta aaccaggttt cgatcagttt gctgtccacg cgctggcact
    11401 gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt cgccttcgca atgcgagggt
    11461 taccttcagc cataaagatc agcacacatt ttccgtctgc aaaatgggtg aagtgttggg
    11521 tgccatcttc agcgtcatac aaacgagcga tcgacggacg atacccttcc accatgactt
    11581 cacgcaggat gttgaagccg gttttcatgt cttccaggat atagccgtag aagaggttgt
    11641 tttccggggt gaatttaaag attttcactg ttacttcagt gatatagcaa agtgcacctt
    11701 cgttaccgat gatgatgtgg cgaatgtccg tgccagccgc gcggcgtggc acgtttttaa
    11761 tgcgtgtgac ggtgccatca gccaatactg cttccagacc aacgaccata tcttcgattg
    11821 cgccgtagag tgtggagaac tgcccgatac tgcgggttgc taccaggccg cccatctgcg
    11881 ccagcggctt tgactgcgga gaatgccccg tggtgtaacc tttttcacgc aacgcatttt
    11941 ccagcacttc ctggggaacg ccacattgcg ccgtcgcctg catattctca atatcaatat
    12001 taatgatttg attcatggcg gaaccgtcga gcaccaccga gttttctaca acagtttcca
    12061 gcccaccttc agtggcggaa gcgccggtac gcggcacacc gttaattttg tgcgcattca
    12121 taaaattcag cacacgggag acttgttctg tggaaccgag ttttacgacc gctgccggaa
    12181 tcggcaaagt ataaatacca tgaatatccg gaaatttacg aaaacggtca atactgttct
    12241 tctttaatac tgtttcatcg gtaattacgc gatctgcacc aacaatttcc tttagttggt
    12301 cgacaatcgc tgcgcgagat aaagacatag taattccttc ctgataataa aaatggaata
    12361 atcattaata agagaaaaca gaaaccaccg ttatggatta gcgcactaaa taaccaccat
    12421 cgaccactaa taaatgccca ttgacataat ttgaagccgg acttgcgagg aatacagctg
    12481 cgcccattaa atcctgagta tcgccccaac ggtttgccgg aattgatcaa gaacgcgctg
    12541 attggtttct ggattactgc gtgtcgccag cgtaatatct gttgcataat agccaggggc
    12601 aataccattt acctgaatat tatattgacc tagttcatca caataagctt tggtgaatcc
    12661 ggcaagagca tgtttagtgg cagaatatgc aggtgaccat tgtccaccta agtaagagaa
    12721 caatgaacag atattaataa ttttgccgct tttctgcggg atcataattt ttgcagcttc
    12781 atagcttaac tcgaatgcgg cggtcaggtt cacatcaatc atcggatccc agtcggcacg
    12841 accgaagtcc agcaccttat tcagcttaca aataccggca ttgtttacca gaatatcaac
    12901 tgtaccgaaa cgctcacagc aagcagcgat aatcttctgc ggcgcgcctt ctgcggtgat
    12961 atccacctgc atgaagtcca cctcaacacc ctgtttttca atcatttcct ttgtttcgcc
    13021 gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca acgccatggc
    13081 aaatgcctag cctaaaccgc tattcccacc ggtaacaatt gcggttttac ctttcagtga
    13141 gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct ccaatttccg
    13201 taaggcaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt tctcattatc
    13261 tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga tcgtaatcac
    13321 agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta ctctcacaca
    13381 acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag aaaagtaatc
    13441 cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg gaatacgata
    13501 tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt agcggcggcg
    13561 ttagttttga cctggcttat ttacgttata tttatcaaat tcccatggcg aaatttatgg
    13621 gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg gctattattc
    13681 tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg attacttccg
    13741 cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa
    13801 tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga
    13861 ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg ggctggatgg
    13921 aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc
    13981 gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac agtgtggttt
    14041 acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac ctgcgcagtg
    14101 ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt ttgcgtatca
    14161 gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac gccattctga
    14221 gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca gcgagctaca
    14281 tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttggc ggcattatca
    14341 ccacctacag taaagtgaaa tcccctaccc gcgtgatcca aatcctttcc gtactcggcc
    14401 tgctggcgtt aactgccctg ctcgtcacga actctaaccc gcaatcggtc gcgatgggga
    14461 ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg tactgggcct
    14521 gccctggcga agtgagaaca ccgtcttaca ttatgggcac cacggtaggt atttgttcgg
    14581 tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg caagacactc
    14641 tgcccgcagc agaagcctac cgcaatatgt ggctgatggg aatggcggcg cttgccatgg
    14701 tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa
    14761 tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga aaaaatacat
    14821 catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc tggaaggtag
    14881 tgtggtttgc gaaggtaaag gcttattaca gccgatgcac acgccttatg ccgatactgc
    14941 agaacatcct gacgacgatt tatgggcatc attatgtttt gcaggtcacg atttgatgag
    15001 ccagtttgcc gggaataaag aagatattgt tggcatcggc ctgggctcca tccgttgctg
    15061 ccgtgcgtta ttgaaagccg atggcacgcc tgctgcgccg ttgattagct ggcaggatgc
    15121 acgcgttaca cgcccttacg aacatacgaa tcctgacgtg gcgtatgtca cctctttttc
    15181 gggttatctg acgcatcgct taaccggcga gtttaaagac aatatcgcca actattttgg
    15241 tcagtggccg gtggattata agagctgggc atggagcgaa gatgctgcgg taatggataa
    15301 gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg cctggcaccg tcctcggaca
    15361 tatcacacca caagccgcac tggcgacaca tttcccggca ggactgccgg ttgtttgtac
    15421 caccagtgat aaaccggtag aagctctggg ggccggatta ctggatgatg aaactgcggt
    15481 aatttctctt ggcacttaca tcgcattgat gatgaacggc aaagcactgc cgaaagatcc
    15541 ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg
    15601 tattcgcaaa ggtatgtgga cggtgagctg gctgcgtgac atgttaggcg agtcgttaat
    15661 tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaga aagcctctag
    15721 tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca atccgtggga
    15781 accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg catggatata
    15841 tcgttcgata ttggaaagcg tggcgctaac gctgaagaac aattacgaca atatgtgtaa
    15901 tgaaatgaat cactttgcga agcatgtgat cattactggc ggcggttcga acagcgatct
    15961 gtttatgcaa atttttgccg acgtgttcaa ccttccggca cgccgtaacg ccattaacgg
    16021 ttgtgcaagt ctgggagcag cgattaatac agcggtaggt ctggggctat acccggatta
    16081 cgcaacggct gttgataaga tggttcgcgt gaaagatatc tttataccga ttgagagcaa
    16141 tgccaaacgc tacgacgcga tgaataaagg cattttcaaa gacctaacca aacatactga
    16201 tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatg tggattcgat
    16261 ccagagctgg tcgaatgcgt gaatgaggta ggccgggcgg aaaactgccc ggcctgaaca
    16321 tacctaaatg gttatccccg ctgacgcggg gaacataagt tcgacaactt aaaaaatgat
    16381 aacaagtccg gtttatcccc gctgatgcag ggaacataat aaaaacttta actagttcgg
    16441 tttatccctg tttttacaag gaatattaac tacactcgat gtctttagaa ttaaagacaa
    16501 tacgaatcgc actttatcaa ttttcactta aagtatgaac tggatatctt tattcataag
    16561 aactattcat caacagcgta tagaggcggt tatgtaaaac cactcattag cctcaaaaca
    16621 aagacaaact tccagattta ttacttaa
//
LOCUS       DAZKGS010000099        16465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKGS010000099 DAZKGS010000000
VERSION     DAZKGS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..258
                     /locus_tag="ACHOQY_003176"
     CDS             <1..258
                     /locus_tag="ACHOQY_003176"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9679227.1"
                     /translation="WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAID
                     FDARTAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
     gene            320..589
                     /locus_tag="ACHOQY_003177"
     CDS             320..589
                     /locus_tag="ACHOQY_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9679228.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            558..1676
                     /locus_tag="ACHOQY_003178"
     CDS             558..1676
                     /locus_tag="ACHOQY_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9679229.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1843..2637
                     /gene="potC"
                     /locus_tag="ACHOQY_003179"
     CDS             1843..2637
                     /gene="potC"
                     /locus_tag="ACHOQY_003179"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9679230.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2634..3680
                     /gene="potD"
                     /locus_tag="ACHOQY_003180"
     CDS             2634..3680
                     /gene="potD"
                     /locus_tag="ACHOQY_003180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9679231.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3738..4199
                     /locus_tag="ACHOQY_003181"
     CDS             3738..4199
                     /locus_tag="ACHOQY_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP9679232.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            4196..4984
                     /locus_tag="ACHOQY_003182"
     CDS             4196..4984
                     /locus_tag="ACHOQY_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9679233.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHOQY_003183"
     CDS             complement(5123..5944)
                     /gene="cobB"
                     /locus_tag="ACHOQY_003183"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9679234.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHOQY_003184"
     CDS             complement(5960..6871)
                     /gene="nagK"
                     /locus_tag="ACHOQY_003184"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9679235.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHOQY_003185"
     CDS             complement(6900..8144)
                     /gene="lolE"
                     /locus_tag="ACHOQY_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9679236.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHOQY_003186"
     CDS             complement(8144..8845)
                     /gene="lolD"
                     /locus_tag="ACHOQY_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9679237.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHOQY_003187"
     CDS             complement(8838..10037)
                     /gene="lolC"
                     /locus_tag="ACHOQY_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9679238.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10299..11372
                     /locus_tag="ACHOQY_003188"
     CDS             10299..11372
                     /locus_tag="ACHOQY_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9679239.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11500..14946
                     /gene="mfd"
                     /locus_tag="ACHOQY_003189"
     CDS             11500..14946
                     /gene="mfd"
                     /locus_tag="ACHOQY_003189"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9679240.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHOQY_003190"
     CDS             15090..16052
                     /gene="ldtC"
                     /locus_tag="ACHOQY_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9679241.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHOQY_003191"
                     /gene_synonym="ycfR"
     CDS             complement(16135..16392)
                     /gene="bhsA"
                     /locus_tag="ACHOQY_003191"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9679242.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4165 a         4233 c         4140 g         3927 t
ORIGIN      
        1 tggggaaatg gagccaactt cgacaacacg attttgcgcc gttcatacga acggcagggg
       61 atcccctgcc cgtggcgtta ctacaacgat cgcgatgtac gcacaatcgt tgagctgggg
      121 aaagccatag acttcgatgc cagaacggct attccattcg aaggtgagcg ccataatgca
      181 cttgatgacg ctcgtcacca ggcaaaatac gtttcagcta tctggcaaaa actgatcccg
      241 aatcaggctg atttttaatg ttcaaccgtc gccagttgtc gttgatattc tgcaactggc
      301 gcgttccgga gtgatagcca tgagcgaaca gtacctgata acgctcgacg agtggaaacc
      361 aaaacggttc agtctcccaa taacaaacac taccctggtg aaatacggaa aactaggata
      421 catcgttcca agaccacaaa aaattcgtgg gcgttggctg atagatcgcc gagcagtatt
      481 tgttgggcct ggtgaaacgg gaattgcgcc ggaaattcat actggcgatg atgatgcact
      541 gaaggagatt ttaactcatg tcaccgaggc cacgaaaaaa cagcactgac gtagccggtc
      601 tttacgaaaa gtttgatcgc agaactggca gagtttacta ccagtataaa aatcctgtga
      661 ctggaaaatt tcacggactc ggaacagaca aaggtaaggc agaaaaaatc gcttccacag
      721 ccaatcagcg aatagctgca gcagaagctg aatatttcat gcgcaaaatt gatgaaagtc
      781 cgtcagcaac aaaacgtcgg ggtatcagat taaaggcatg ggttgatcga tatctgaaaa
      841 tacaggacac gcgactgaaa aatggagata ttgcagctac aactcacaaa gaaaaaaccc
      901 gaatggctgc atacctggtt tcccgtctgg gaaaccaccc attgaaagaa ctggaagtaa
      961 gagactttgc attaatactg gatgagtggc tggataaaga catggtcagc acagcgagag
     1021 taaatcgtgg attatgggtt gatatttata aagaagcaca gcatgcaggg gaagttcctc
     1081 ctggatggaa tcctccggag gctacccgta aaccgatccc taaagtaacc agagccaggc
     1141 tcaccatgga agactggcaa aaaatttaca atgcaacgcc tgaaaaacac tttatccgta
     1201 acgcaatgct tcttgcgatt gttactggtc agcgccgtga tgacatttgc cacatgcgtt
     1261 tttcagatgt gtggaacgaa cacttgcata tcacccaggg aaaaaccgga atgcgtctgg
     1321 cgttaccgct tacactacgc tgtgatgcca ttgggataac gttaaaagaa gttattgatg
     1381 ggtgccgaga cagaatatta agtccatatc taatccatag tcggcaccag aaacaaccga
     1441 agccgatgag taaagacaac ctgagcgact actttgccaa agcacgggat ctggctggga
     1501 taattccacc agcaggaaaa actccgccaa catttcatga acaacgctct ctatcagaac
     1561 ggctgtaccg tgcacagggt attgatacaa aaacattact aggacataaa gtccaggcaa
     1621 ccaccgatcg ctataacgat actcgaggtc aggaatgggt taagttggtt atttgataaa
     1681 caccatatgg aatatccgtc accatctcag gaaaaaagtg ttgtaaaatg cgtggcctag
     1741 ttttggggag acgttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat
     1801 cctgaaagtg tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
     1861 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
     1921 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
     1981 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
     2041 gcggtgtttt cggcgacatt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
     2101 cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca
     2161 ccagatatcg tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt
     2221 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
     2281 gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
     2341 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
     2401 gcgggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcgttcgtc
     2461 accggaccaa gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
     2521 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt
     2581 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
     2641 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
     2701 ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
     2761 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
     2821 aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt
     2881 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
     2941 agttaacgaa cttcagcaat ctcgatccag acatgctcaa caagccgttt gacccgaata
     3001 acgattactc cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg
     3061 tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgagtac aaaggcagcc
     3121 tgctgttgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact
     3181 ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
     3241 tgccaaacgt cgcagcgttt aactccgata acccagctaa cccgtacatg gaaggcgaag
     3301 ttaacctcgg catgatctgg aacggttctg ctttcgttgc acggcaggcg ggtacgccaa
     3361 ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg
     3421 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
     3481 tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
     3541 tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
     3601 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
     3661 agctgaaagc aggacgttaa ttcagcaagc gggcggtggc attaccgccc gttttttatt
     3721 tagcaaggat gtggctaatg agtcaggaca gtaaagtgtt atcccgcgct tttctcggta
     3781 tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca
     3841 aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa
     3901 acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt
     3961 acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca
     4021 gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg
     4081 ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc
     4141 tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc tgcccatgaa
     4201 aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc
     4261 attgattcca ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga
     4321 agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt
     4381 gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc
     4441 gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg
     4501 cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca
     4561 tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca
     4621 gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc
     4681 actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa
     4741 cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta
     4801 cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg
     4861 ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact
     4921 ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg
     4981 ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc
     5041 ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc
     5101 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
     5161 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
     5221 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     5281 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
     5341 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
     5401 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     5461 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
     5521 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
     5581 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
     5641 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
     5701 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     5761 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     5821 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     5881 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     5941 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
     6001 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     6061 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
     6121 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     6181 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
     6241 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     6301 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     6361 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
     6421 tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc
     6481 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa
     6541 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
     6601 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
     6661 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
     6721 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     6781 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
     6841 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     6901 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     6961 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     7021 aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc
     7081 aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc
     7141 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     7201 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     7261 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     7321 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
     7381 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     7441 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     7501 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     7561 aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     7621 ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc
     7681 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg
     7741 ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     7801 acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc
     7861 gcggcagcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc
     7921 cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc
     7981 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
     8041 gcaatgccaa tggtagaaat aacggagatc agcgacacca tgccgccgcg ccgccgtccg
     8101 cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca
     8161 ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg
     8221 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     8281 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
     8341 ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc
     8401 cgccagaaag ttcagatggg cggtgattcg cacgatgatc cagccccacc gcttttaaca
     8461 tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaatcagc agcggcatag
     8521 ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca
     8581 gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
     8641 taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc
     8701 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
     8761 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
     8821 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
     8881 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
     8941 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
     9001 atcggcatca gattatttaa ctggctggca agcagtgcgc caagcgccgc accgaggatc
     9061 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
     9121 gttaagcctt gcgtttgcag gatcgcgact tcgccctgct tctccattac catcagccct
     9181 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc
     9241 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
     9301 catttgctgc cttcaggcag tttttgctga cttaaagagt caactttcag cggctcatcc
     9361 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
     9421 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc
     9481 acattgaaca ggcgctggct tggaatacgc cccatcggcg tgaactggct ggcagatggt
     9541 accatcacgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
     9601 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggt
     9661 gtaagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
     9721 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
     9781 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
     9841 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
     9901 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
     9961 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    10021 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    10081 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    10141 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    10201 tctgaaaaat catccaatac agacagatcc ccattacacc tcgggtcata acccgcagga
    10261 tcaaccgcat gtaaattaaa aaatagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    10321 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    10381 tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    10441 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atatccggct
    10501 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    10561 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    10621 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    10681 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    10741 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    10801 tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    10861 tgaacagtgt gatccgcaat ttgctttatt acagccttgg cgcatggttt ggcgcaacaa
    10921 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    10981 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    11041 ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca
    11101 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    11161 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    11221 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    11281 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc tccccgcctt
    11341 cactgcccgc tgccgtcagt tactctcgtt aaaccatcgc cccagccggg gcgattcaat
    11401 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    11461 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    11521 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    11581 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    11641 gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg
    11701 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    11761 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    11821 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    11881 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    11941 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    12001 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    12061 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    12121 gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    12181 cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    12241 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    12301 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    12361 ctggaaacca gtgccgaacg tttccaggct gacacgctgg cgcgttttga gaatcgcggc
    12421 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    12481 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    12541 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    12601 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    12661 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    12721 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    12781 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    12841 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    12901 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    12961 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    13021 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    13081 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    13141 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    13201 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    13261 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    13321 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    13381 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    13441 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    13501 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    13561 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    13621 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    13681 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gtattaaagc gatgcgcgcg
    13741 aacgtggata ttctgacgct tactgcaacg ccgatcccac gtacgctgaa tatggcaatg
    13801 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    13861 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    13921 cgcggaggac aggtttatta tctctacaat gatgtggaaa acattcagaa agccgccgaa
    13981 cggctggcag aactggtgcc agaagcgcgg atcgccatcg gtcacgggca gatgcgcgag
    14041 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    14101 acaaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgc
    14161 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcacat
    14221 catcaggcat atgcatggtt gctgacgccg catccaaaag cgatgactac cgatgcacaa
    14281 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    14341 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    14401 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    14461 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    14521 atgccgtcgc tattgccaga tgatttcatt cctgacgtga atacgcgttt gtcgttctat
    14581 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    14641 gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc
    14701 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    14761 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    14821 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    14881 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    14941 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    15001 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    15061 aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    15121 taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct
    15181 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
    15241 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
    15301 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
    15361 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
    15421 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
    15481 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
    15541 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
    15601 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
    15661 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
    15721 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
    15781 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
    15841 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
    15901 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
    15961 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
    16021 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcaaca
    16081 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
    16141 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
    16201 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
    16261 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
    16321 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
    16381 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
    16441 atcgataact aaatgataga tgtga
//
LOCUS       DAZKGS010000100        16282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKGS010000100 DAZKGS010000000
VERSION     DAZKGS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..1427)
                     /gene="yihO"
                     /locus_tag="ACHOQY_003192"
     CDS             complement(24..1427)
                     /gene="yihO"
                     /locus_tag="ACHOQY_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679243.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            complement(1470..2855)
                     /gene="yihP"
                     /locus_tag="ACHOQY_003193"
     CDS             complement(1470..2855)
                     /gene="yihP"
                     /locus_tag="ACHOQY_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679244.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            complement(2901..4937)
                     /gene="yihQ"
                     /locus_tag="ACHOQY_003194"
     CDS             complement(2901..4937)
                     /gene="yihQ"
                     /locus_tag="ACHOQY_003194"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP9679245.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            complement(5136..6038)
                     /gene="yihR"
                     /locus_tag="ACHOQY_003195"
     CDS             complement(5136..6038)
                     /gene="yihR"
                     /locus_tag="ACHOQY_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP9679246.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(6176..7417)
                     /gene="yihS"
                     /locus_tag="ACHOQY_003196"
     CDS             complement(6176..7417)
                     /gene="yihS"
                     /locus_tag="ACHOQY_003196"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP9679247.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(7434..8312)
                     /gene="yihT"
                     /locus_tag="ACHOQY_003197"
     CDS             complement(7434..8312)
                     /gene="yihT"
                     /locus_tag="ACHOQY_003197"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP9679248.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(8336..9232)
                     /gene="yihU"
                     /locus_tag="ACHOQY_003198"
     CDS             complement(8336..9232)
                     /gene="yihU"
                     /locus_tag="ACHOQY_003198"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9679249.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            9400..10296
                     /gene="yihV"
                     /locus_tag="ACHOQY_003199"
     CDS             9400..10296
                     /gene="yihV"
                     /locus_tag="ACHOQY_003199"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP9679250.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            10330..11115
                     /gene="csqR"
                     /locus_tag="ACHOQY_003200"
     CDS             10330..11115
                     /gene="csqR"
                     /locus_tag="ACHOQY_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9679251.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            11214..11813
                     /gene="yihX"
                     /locus_tag="ACHOQY_003201"
     CDS             11214..11813
                     /gene="yihX"
                     /locus_tag="ACHOQY_003201"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9679252.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            11807..12679
                     /gene="yihY"
                     /locus_tag="ACHOQY_003202"
     CDS             11807..12679
                     /gene="yihY"
                     /locus_tag="ACHOQY_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9679253.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            12676..13113
                     /gene="dtd"
                     /locus_tag="ACHOQY_003203"
     CDS             12676..13113
                     /gene="dtd"
                     /locus_tag="ACHOQY_003203"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9679254.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            13158..14099
                     /gene="fabY"
                     /locus_tag="ACHOQY_003204"
                     /gene_synonym="yiiD"
     CDS             13158..14099
                     /gene="fabY"
                     /locus_tag="ACHOQY_003204"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9679255.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(14163..15071)
                     /locus_tag="ACHOQY_003205"
     CDS             complement(14163..15071)
                     /locus_tag="ACHOQY_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9679256.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            15300..15611
                     /locus_tag="ACHOQY_003206"
     CDS             15300..15611
                     /locus_tag="ACHOQY_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9679257.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            15612..15902
                     /gene="nadS"
                     /locus_tag="ACHOQY_003207"
     CDS             15612..15902
                     /gene="nadS"
                     /locus_tag="ACHOQY_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9679258.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
BASE COUNT         4022 a         4057 c         4253 g         3950 t
ORIGIN      
        1 atggaaatag cggctattga catttataac gttacggaag ccgttttttc ttctgtcgcc
       61 atctctttat ttttgcgttg gttgatttcc tcaataatca atgcaaaccg tttttcattg
      121 agtgtgtaaa agaatcccat cgtcaatgca gcaataattg ccagtgcaca aggccagata
      181 aaaatgagct gacgcaaacc ctgtaatgtc gcatcgcttt gcgcgatgtt gggaacataa
      241 ccaatttgcg tcagcataat gcctggcaag aagccagcaa gtgcggcaga aattttacga
      301 aagaaggtat aaccggtata aacagaacct tcggcacgaa taccggtttt ccattcaccg
      361 taatctacgg tatctggtac cagtgcccag ttcaggctgt ttacaaaggc cgtgccaaaa
      421 aaggcgacac aagagaacat gacgaaagtg aaagagttac ttccccagaa ataattcagt
      481 atatcaccca ccgcccatag caccatgcca gctaaataaa cctgtttttt accaaaacat
      541 tttacagtta ctggcaccag taatacgccg acgaggatgc atcccatact gaagaatccc
      601 atccacgaca ataaattaat atcattcagc acatactggg tgtaatagac ctgaatcgcc
      661 agtttgatat taaatgccgc cagagtacac aggttagcaa tgcataaaac cagcaatggt
      721 ggattacgaa atatcgcgca aaaagatttg agaatactgg ctttatgtcc ggttggcgct
      781 gtgtccacat aatgctcttt gacgcctctg tagcacagca tcataaaaat aaagccgcca
      841 atggagaaca taagtgccgc acaggcataa ccgacggttg agtcagaaaa aagcgactgc
      901 aacggaataa acgccacggt acaaatcaat agccctatgg ttgcaccacc ctggcggtaa
      961 gccgcgagct gcgcgcgctc attcgggttt ttggtaattg ccgggatcat cgcaccatac
     1021 gagcagttca tcagactgta tgaaagtcca aacatcataa acagcgcggt ggcaatcgtc
     1081 gttttaaccg gcaaacaaaa ggtggtggcg ataaactgga gcgttgcaat taacgccgcc
     1141 ggaactgctg catataaaat aaagggacga aatttgcctt taggcccaat atttttccgc
     1201 gagtcgagta aaaatccggt gagcatatcg gtaaacgcgg taaaaaactt cgccaccaga
     1261 aagataatcc cgccatagta agcaggcatc cctaattcat cggtataaaa tttgaggaga
     1321 tacagagtac cgatgcagag cattaaattc gaaccgacgt cgcccatacc ataggcgatt
     1381 ttttcccgca gattcagttt taatgtcagt ggattatggt cagacataat cactccttat
     1441 aacgccccgc agggcgtcgt ttattattat tacgccgtgc gtttacgggc ttctatctct
     1501 tccacaatgc ggacatacat cttctcgttc aggctgtaga agcaacccat cgccacaatg
     1561 gtgactaccg ccagtgcgct tgggtagatg aagatcaact ggcgtaaccc ttcaatggtg
     1621 tggtcagcct gtgcgacgtt cggcacatag ccaatttgcg tcagcatcca gccggggaag
     1681 aaaccagcca gcgcctgaga cactttgcga aagaaggtga agccggtgta gaccgtgcct
     1741 tccgaacgca cgccggtgcg ccactcgccg tactcgacgg tgtcggaaac cagcgcccag
     1801 ttcaggctgt taacaaacgc tgagccgaag aacgccaggc aggagaacgc cacaaagctg
     1861 accgagccgc cgccgaagaa atagttgagc agatcgccca gcacccaaat cagcaggccg
     1921 ccgatataga ccttcttctt accaaaacgt ctgactgcgc caggcatcag gaacacgccg
     1981 atgaaaatac agcccatgct gaaaaatccc atatacgaca acaggatggg atcgttaagt
     2041 acgtactggg tgtaatagac ctgaatggcg agcttgacgt taaacgcccc taaggtgcag
     2101 aggttggcaa tacacagaat gaacagtggg cggttaccgg caattgcgcg gaaagattgc
     2161 aacaggccag gcttttgcgc cggattggtt ggttgggttt cgacataacg ctctttcacg
     2221 cctgagtagc agatccacat aaacagcagg ccgaacagtg aaaacagcgt ggcggcgaag
     2281 atatagccaa gttgctgatt accctcgata agattcatca ctggcacgaa tcccaccgtg
     2341 cacagcagca ggcccagcgt agcgccgccc tgacgccatg ccgccagtga ggcgcgttcg
     2401 ttggggtttt tggtgatagc gggaaccatt gcgccgtagg agcagttcat catgctgaaa
     2461 aacagtccgt aaagcataaa cagaatagtg gccatcaccg ttttaccggt gacatcaaac
     2521 ggtgtgccga caaagttggc gatcgccagt aaggtgaccg ggaatgacgc atacagaata
     2581 aaaggacgga acttaccttt tggaccgatc ttgcgtcggg aatccaacat aatgccggta
     2641 cccatatcgg taaacgcagt aaagaatttt gaaatcaaaa agataatgcc gccataggtg
     2701 cctggcagcc ccagaacgtc ggtataaaac ttcaaaagat aaagcgtacc gatatccagc
     2761 aggatgttag aggccaggtc gccaataccg taagagagtt tctctttaaa gggcaggcgt
     2821 aaagttgctg gatcttccgt tgtgatgtga ctcatcgatg ttctcccttt gcccatgagt
     2881 ttccccacgg gcggatgtga ttagatgctt tttaacgacg cgaacagtgc cgcccattcg
     2941 ctatcggcgc gataaaagac cggcggcttg ccgatgggcg cattaacggt aacttccccg
     3001 ccccggaacg cttcacccgt ccaggcgtgg acccagttat cctccggcag atagagcgtc
     3061 cagtcgctac ggccttcttc atgcaccgga gcgaccagaa tgtcgcgacc taacaggtac
     3121 tgatatttca gggtgtaagt gtgcgcatcg tcttcgtaat gcaggaacag cgggcgcata
     3181 accggcaggc cggacttcgc attcagcgcg acagcctctt tcaggtaagg tttcagggtg
     3241 gtgaagacgg aggtcatacg ggcaaaatgg gcgatagttt ctgcgtcgcc gtcaaactgc
     3301 cagttgtcgc caggacggtt accttcgtgg gtgcgcatca tcggcgtgaa ggcgctgaaa
     3361 tcgcaccagc gcagcagcag ctctttgctg cgcttcatct caaacagggt ggtgtaaccg
     3421 ccaatgtcgc tgtggtgcag gccatgtccg gtcattgcca gcgacagcgc cgccgggaca
     3481 accgacgcca ggccatcgtc gagactccag tcgacgttct ggtcgcccgc ccacatcatg
     3541 gtggagtatt tctggctacc ggtagaaccg gcgcgcataa agaaaaggat ctcgccgagc
     3601 ttgcccgttt cttcaagggc ttcgtagtta catttcgccc acagcgcggg ccaggcgtta
     3661 tgcataattt cggcgctgat gccgttatgc aggtaagtgt cggtgggcag atactcgccg
     3721 aagtcagcca tccagccgcc gcagccgagt tcaatcatgt tctttttgat cacttccttg
     3781 aaccaggcgt aggcttctgg attagtgaga tcgacaacgc cgccgtaaaa ctcgccaaac
     3841 tccaccagat agtcaccgcc agaggcatct tttgccagat agccgtgttg tgccgcttct
     3901 tcgcagagat cattatcgct ggcaacatac gggttgatat aggccaggaa ctgcacgccc
     3961 tcctgattcc actgcttaat gcgtgaatcc agttgcgggt agttttcgct gttccacttc
     4021 cagttccaca tcacgcgttt gccaaaagag gtcatacgaa taccggacca gtcctgcgcc
     4081 cagatgccgt tgaccttcac gcccgcgtta cgcatggtgt ccagtttctt ctggcacact
     4141 tccgtcccgc cctgaatgcc gagcgttact ccgtcataaa tccagtcggg cagttctggc
     4201 tggcgtccca gcagggcggt taatttttcc agcagggaaa tgtatgtgtc agcacattca
     4261 aaacgcagcg ttgctttgtc ttcccacagc gccagttcat ggtattccgg ggcactaaag
     4321 tcgaagttca tatagcaact gttatcaaca tggcagtaat acttctgcgt gctgacaaac
     4381 gtaggctgtg ggaagaaagt ccagtaatag tcgccgcccg cattttcttt gcagtcggcc
     4441 tgccaggtga cataggtttg tttgttgcga ccaacgcctt gttcactggt ccatagcggg
     4501 aacggtttgc cacgcagatc gaagtaggaa aactgttcgc cgcagccgta gatatggtcc
     4561 tctggttgag cggcaaggcg cagccagata cggttgtggt taaggttgtc gttttgtagt
     4621 tccagcaata aacggccctg atcgtcggca gagatattca gcgtggcgct aatgtcagaa
     4681 ccacggctga aatgaattaa ccaaccatcc ggtgactggc tgacgatggc gtcggtaagc
     4741 gcaattttct cctgtagttt atctttaatg ctgaaattac cgcggaacat atcgatatcc
     4801 gctatacctg agccaatcca taaacaagga ttatctttgc tatgggttaa aataagacgt
     4861 tgttgaaaat gtagggtaaa actgtcgtta ttctgatgaa attgaaaatc taataactgt
     4921 ggacgtggcg tatccataac taatctccat agagttgaga acgagattgt tgccttcctg
     4981 ttattacagg cttatgtaaa atgattctca taaaactgat atataaaaac tttacagaag
     5041 ctgagctgtt tttaacttaa taaattgaag atacataatg ttgcctttag cgagtgaaat
     5101 gttcttcaat gattttgact gctaatcaat acaagttatt ttctctgtcc gtaaatgtta
     5161 aaaaacaggc ggtgcggttt tcctggttcc agtaataata agtcgatacc tgaattaaag
     5221 gcgttaggcg ggcaactcat aggttcaact gccagtccct gacgctgtaa tttttcaccg
     5281 ctataaattt gtacccaggg ttgatcgcaa caaaggctaa ccgaaagcgc ctgttgtgga
     5341 tgggtaatgg ttatttccca cagatcatta gcggttttaa aggtgtggtc gatttttgtc
     5401 gcggctattg ttttcgcctg cgtgaaattt aaatccagct catcaacgtg gtgaagcgtt
     5461 gtcggattta cgtgttcatc aacggcataa acctggtttg ccggaagctg gaacagatat
     5521 tcatcaacag atgttaagtt gcaggttaaa taagtatgaa tacccacgcc ataaggcgct
     5581 gcgacagtgc cgatattctg gcttgcgatc tcaacggaaa gacccgtgtg tgcgttgagc
     5641 gaataaacaa cttgtgaagc cagcataaac ggatagccgt aactgggcgg tagaaaagct
     5701 gtcagggtaa cgctggtggc ggttaattca ctaatttgcc agtctcgcca ggcaagcagc
     5761 ccgtgaatag cggcttttga actatgttca ttaatgggta actgatattc ctgcccctga
     5821 tagcggtaac agccgttggc gatccggttc ggccacggaa taagcacttt acccagatgc
     5881 gccagcggca tctcttcggg tttgtgcggg atgactaaat gacaaccctg aaaggttaat
     5941 tcagccagac cagcaccgac ggtcacaatg gtcgcgtgat aatcccccgc ggcaagtgaa
     6001 acggtctgtc cactgcaatg catattggtg atttgcatac aaggcacctt aattaacgac
     6061 atacagcctc ccgtgaggct gatgtcaaac ccaaaaactg caccggacgg tcccctcgcc
     6121 cctttgggga gagggttagg gtgaggggaa aacccgcacc ctattttcag atcagttatt
     6181 tcgcattaat atccagcaga cccgctgcaa ccgctggagc catgccgggc gctaacggga
     6241 tacgcgggat caccaggcaa tgcagcaggt gataaatatc ctgtttgccg tcccagactt
     6301 tggtggtgac cttattgtcc gcatccagct cctgccacca ggaaccattt tcatagtcca
     6361 tcaggtactt aatgcagtac tcccaccatg tttgatacca ggtttcatac tggcgatcgc
     6421 cagtgacggt gtagagcgcg taggccgtac ccattgcttc gacgataggc caacgtacac
     6481 gttcgcggac caccggtttt ccttcccagt caacggtata aacaatcccg tccgcgccat
     6541 cgggtgccca ggcatcgcgc acggtggcgt taaacagacc tttggcatct tctagcagcc
     6601 atgctggtgg ttgttcgcaa cgggcttcca gggcagcatg gatgtgcagc attaaacggc
     6661 cccattcgat ccagtggcct ggtgtacctc cgaacgcgcg gaagcgatgc gccgggttat
     6721 ctttgttgta atccggcagc ggattccact gggtatcgaa gtgttcgtta acgcgataat
     6781 gattatttct ggcgacgtcg tggataatca cggaagccac gcgaatcgcg cgatccagcc
     6841 attttttgtc gtgagtgacg tcataaacaa tcaagaaagc ttccaccgcg tgcatattgg
     6901 cattgccgcc gcggtactct tcggttttgc tgaaggcttc gtcccaggat tccaggcaca
     6961 tctgctcttc ttcgctccag aaatatttct cgataatttc aatggtgtaa tcgagcagct
     7021 tgcgcgcttc cgggtgaccc gttgtgacgg cgctggcggc acccagcaga gcaaagaagt
     7081 gttgatagcc ctgtttggag gcatccacca cgccctcgtc atttacgcag gcataccagc
     7141 cgccatattt tttatcacgc agtgcgccgt tcatggcttt gataccgtga tcaaccaacg
     7201 agtaagcgcc aggtcgaccc atcgccgcag caacggaata aacgtgtaac atacgagcgg
     7261 tgatccacag atgggtgccc atctcttctt tgatttgccc tttattgcct aaccagccaa
     7321 aaccagtcgg cactactgaa tttttaccaa aatcaaagat gcggtcggtt tcctgttcca
     7381 gccaacggtt gtggcttagg gtgttaaacc atttcattcc ttttatcctc atcttagcgg
     7441 cgtttagcca tcatttcgtc gacgatatcg ccaagttgtt gtaatttcgg tgcgcaaacg
     7501 tcacgcagca tcagctcgtt gtctggtaaa ccgacgaccg atgcccagac tgcacggcct
     7561 gccaggaatc ccgatgcgcc cgccgtcatt gccacgcgta cggcacgcgg gaacagtttt
     7621 tcgtcgacac cggaagagag gatcacccat ggcatattga tatggtcatt cagacgttgt
     7681 gaagcacaga gaagctcttg ttgcggacct ttgccataaa gcggcatttc aactttgtag
     7741 aggtcagcgc cactgtcgcc cagctctttg gcggcatcga tgatcgcttg ttcgcgatcg
     7801 aatttatcgc cacgacgcgg tgggcggacg actggctcaa tgatgcttac cagaccgtgt
     7861 gagtggcaca gttcgttgaa ctctttcacc atatccagac gttgctgcgc atcttcatca
     7921 ctacgccaca gcaccagcag ttttaaggct ttgccaccgt cttgtttgat ctgtagcgga
     7981 ttgattttgc ggtcaataac cacgctatcg accggaatac cgttgccagg aatgaactca
     8041 tcggcggcga caatcatggc gcaacttttg gcaatcgcgt tttgctcaac cacctggcgg
     8101 tagcagaatt gttgatctac cagaatcgcc gaggcataag gcgagagggc ctttgcagcg
     8161 ttaactttga aatcagttaa aacgctatcg gctaccggag cgggtgcccc agccgcggca
     8221 aacatcatgc gcatggcttc gcgctgatcg accgccagca tggcaaaacc gcccgatgcg
     8281 cgcgtaatgt cgttgatggt gtacttattc attcatttat tcctttatcc agtcgttaca
     8341 tttttacttt ggcagtcatc ccggcactga cacggacctg ttccagaatg gcggaccagt
     8401 cctggcgacc gcgacccgct gcgcgcgcct ggctataaac ctcccgtgag gcggccccca
     8461 gcggcattgg cacatgcagc tggttggcga catcaagggc gatgccaaga tccttatggg
     8521 caagatcgat catgaaggcg ggagaaagat ctccgctgag gactttgttt ggccaggaag
     8581 tggtgaagtg acctttaccg gcggcggtac cgctcatcac tttgacggca acatcgaagg
     8641 gaagattcag ggcttcgcac aaaacggcgg cttctgccga aagcgcattg agcgcgatgc
     8701 tcatgtagtt gttgatgagc ttaacgcgga tccccatgcc cggaccgcca gcgttgatca
     8761 actcactgcc catcgccatc aggatcggtg tggcacgttc aacttgttca gcggtgccgc
     8821 cagccagcag taacagagta ccggtaatgg catttgcaga agtacggcct accggaacat
     8881 ccatcatgct gaagcctttg gcttgcatat cggcaatcaa tttatcggtt tgcagcggat
     8941 ggatggtgga catatcaatg accagcgcat cggtagataa gccttcgcaa acgccgtttt
     9001 caccgaacaa cacgttgcgc accagatcgc cattcggcaa catggtaatg ataaattcgg
     9061 catctttagc ggcctgcgcc gggttggcgg cgggagtcgc acctttgtct accagatgcc
     9121 gcacagcttc ggcgttcaca tcaaagacgc gaagttgatg cccttgctgc aataaattgc
     9181 tcgccattgg cgaacccatt tgtcctaaac cgataaacgc gattgctgcc ataaccctct
     9241 cctgaataca gtgatgtcac tttttgtcat ttatgacatg ctttgcttgt ctgtttttga
     9301 tcgtatttgt aatttatcgt caaaaaattg acagccgtca ctttttaaac aattggtgaa
     9361 attaaaatga acgcatccca aaatgtttaa ggaatgacca tgattcgtgt tgcttgtgta
     9421 ggtataaccg tgatggatcg catctattac gtggaagggt taccgacgga gagcggtaaa
     9481 tacgtggcga gaaattatac ggaagttggt ggcgggccag cggcgacggc agcggttgcg
     9541 gcggcaaggc tgggggcgca ggtcgatttt attggtcgcg taggtgatga cgacacgggc
     9601 aacagcctgc tggcagagct ggaatcctgg ggcgttaaca cccgttacac caaacggtat
     9661 aaccaggcga aatcttcgca atccgccatc atggtggata ccaaaggcga gcggataatc
     9721 attaactacc ccagcccgga tctgctgcct gacgcagagt ggttggagga aattgatttc
     9781 tctcagtggg atgttgtgct ggcagatgta cgctggcacg acggcgctaa aaaagccttc
     9841 accctggccc gtcaggcggg tgtgatgacc gttctggacg gggacattac gccgcaggat
     9901 atcagtgagc tggtggcatt aagcgatcac gcggcctttt cagaaccggg tctggcgcgc
     9961 ttaacgggcg tgaaagagat ggccagtgcg ctaaaacagg cacaaacgct cacaaatggt
    10021 catgtctatg tgacccaggg tagcgcaggc tgcgactggc tggaaaatgg tgggcgtcag
    10081 catcaaccgg ccttcaaagt tgatgtggta gataccacag gtgcgggtga tgtttttcac
    10141 ggcgctttgg cggtggcgct ggcaacaagt ggggatttag cggagtcagt ccgcttcgcc
    10201 agcggtgtag cggcgttaaa atgcacacgt cccggtggac gagccgggat ccctgactgt
    10261 gatcaaaccc gatctttttt gtcacttttt gtataaaatg ccagggtgat ggtttttcga
    10321 ggaattttca tgagccttac cgaactaacc ggtaacccgc ggcacgacca actcctcatg
    10381 ctgatcgccg agcgtgggta tatgaatatt gatgagctgg caaatctgct ggatgtctcc
    10441 acgcagacgg tccgccggga tattcgtaaa ttaagcgagc aaggcctgat tacgcgccat
    10501 cacggtggcg cgggtcgggc ttccagcgtc gttaatacgg cgttcgagca gcgtgaggtt
    10561 tcgcaaaccg aggaaaaaaa agcgattgcc gaagcggtgg cagactatat tcctgatggt
    10621 tcaacaatat ttatcaccat tggtacgact gttgagcatg ttgcccgggc gttacttaac
    10681 cataatcatt tgcggataat caccaacagc ctgcgtgtgg cgcatattct ttaccacaac
    10741 ccgcgctttg aagtgatggt gcccggcggt acgttgcgct ctcataatag cgggatcatt
    10801 ggcccttcag cggcgtcctt tgtggctgat tttcgtgctg attatctggt aacaagcgtt
    10861 ggggcgattg agagcgatgg cgcgttgatg gagtttgatg taaacgaagc taacgtggtg
    10921 aaaacgatga tggcgcacgc gagaaatatt ctgctggtcg ccgatcacac taagtatcat
    10981 gcttcggcgg cggttgaaat tggtaacgtg gcacaggtca ctgcgctctt taccgacgag
    11041 ctgccgcccg ctgcgctaaa atcacgctta caagacagcc aaattgaaat cattcttccc
    11101 caggaagacg cgtagatttt tgtgaccgtt aacctggctt cataccttgc cacatagcca
    11161 aacccatcct ttcccgctac agttaatttc ttgtggcgcg aaaggaggca aaaatgctct
    11221 atatctttga tttaggtaat gtgattgtcg atatcgactt taaccgtgtg ctgggagcct
    11281 ggagcgattt aacgcgtgtt ccactggcta cgcttaagaa gagtttccac atgggggagg
    11341 cgtttcatca gcatgagcgt ggggaaatta gcgacgaagc gttcgcagag gcgctgtgtc
    11401 atgagatggc cctgccgcta agctacgagc agttctctca cggctggcag gcggtgtttg
    11461 ttgcgctgcg cccggaagtg atcgccatca tgcataaact gcgtgagcag gggcatcgcg
    11521 tggtggtgct ttccaatact aaccgcctgc ataccacctt ctggccggaa gaatacccgg
    11581 aaattcgtga tgctgctgac catatctatc tgtcgcaaga tctggggatg cgcaaacctg
    11641 aagcacgaat ttaccagcat gttttgcagg cggaaggttt ttcacccagc gatacggtct
    11701 ttttcgacga taacgccgat aatatagaag gagccaatca gctgggcatt accagtattc
    11761 tggtgaaaga taaaaccacc atcccggact atttcgcgaa ggtgttatgc taaaaaccat
    11821 tcaggacaaa gccaggcacc gtacccgtcc actatgggcc tggctaaaac tactctggca
    11881 acgtattgat gaggacaaca tgacaaccct ggcaggtaac cttgcctatg tgtcgttgct
    11941 ctcattagtg ccgctggttg ccgttgtttt tgcgcttttc gccgcttttc ccatgttttc
    12001 cgacgtcagc attcagttac gtcactttat ttttgccaac tttctgcctg ctactggcga
    12061 tgttattcag cggtatatcg aacaatttgt tgccaattcc aacaagatga ccgccgttgg
    12121 ggcgtgcggg ctgatcgtca ccgcgttatt gctgatgtac tccatcgata gcgcgttgaa
    12181 taccatctgg cgcagtaaac gagcgcgacc aaaaatttac tcgttcgccg tgtactggat
    12241 gattttaacg ctggggccgc tgctggcagg ggccagtctg gcgatcagtt cctatttgct
    12301 ctccctgcgc tgggcgagcg atctcaatac tgtcatcgat aacgtgctgc gtatttttcc
    12361 gctgctgttg tcgtggatct ccttctggtt gctgtacagc attgttccta ccatccgcgt
    12421 acctaaccgc gacgcgattg tcggcgcgtt tgtcgccgcg ctcctgttcg aagcaggaaa
    12481 gaaaggtttc gcgctttata tcaccatgtt cccgtcatat cagctcattt acggcgtgtt
    12541 ggcggtgatc cccattctct ttgtttgggt ctactggaca tggtgtatcg tcttgcttgg
    12601 cgcggaaatt actgtcactc tcggggaata ccgcaaacta aaacaagcag ctgaacaaga
    12661 agaagacgac gaaccatgat tgcattaatt caacgcgtaa cccgtgccag cgtcaccgtg
    12721 gagggagaag tgacgggcga aattggcgcg ggacttttgg tgttattggg tgtcgaaaag
    12781 gatgacgacg aacagaaagc aaaccgtctg tgcgagcgtg tgctcggcta ccgcatcttt
    12841 agcgatgccg aaggcaagat gaatctcaac gtgcaacagg cgggcggcag tgtactggtg
    12901 gtttcccagt ttaccctcgc cgcagatacc gaacggggga tgcgcccaag tttctctaaa
    12961 ggcgcatcac cggatcgcgc agaggcgtta tatgattatt tcgtcgaacg ctgccgccag
    13021 caagagatga acacgcaaac aggacgcttc gctgccgata tgcaggtatc gctggtcaat
    13081 gatggccccg tgacattctg gttgcaggta tgagccagct tccagggttg tcacgggaaa
    13141 caagagagag tatcgctatg tatcaccttc gggttccaca aacagaagaa gagttagagc
    13201 gttactatca gtttcgctgg gaaatgttgc gtaagcccct gcatcaacca aaaggttcgg
    13261 aacgcgacgc gtgggatgcg atggcgcatc accagatggt cgtcgacgag cagggtaatc
    13321 tggtggcggt aggccgattg tatattaatg ccgacaatga agcgtccatt cgctttatgg
    13381 ccgttcaccc ggacgtgcag gacaaagggt taggaacgct gatggcgatg accctggagt
    13441 cggtggcgcg tcaggaaggc gttaagcgcg tgacctgtag cgcccgtgaa gacgcggtgg
    13501 agtttttcgc caagctgggg tttgttaatc agggagaaat caccacgcca accaccacgc
    13561 cgattcgcca ttttttgatg attaagcccg tcgccactct ggatgatatt ttgcatcgcg
    13621 gcgactggtg cgcgcagctg caacaggcgt ggtacgaaca tatcccgctt agtgaaaaaa
    13681 tgggcgtgcg tattcaacaa tataccgggc aaaaatttat caccaccatg ccggaaaccg
    13741 gtaatcagaa tccgcaccat acgctgtttg ccgggagttt attctcactg gcaacgctca
    13801 ccggttgggg gcttatctgg ctgatgctgc gcgaacgcca tctcggcgga acgattattc
    13861 tggcggatgc gcatatccgc tacagcaagc cgattagcgg taaacctcat gcggtagccg
    13921 accttggtgc cttaagcggc gatctcgacc gtctggcgcg cggacgaaaa gcacgggtgc
    13981 agatgcaagt tgaaatcttt ggcgacgaga cgccgggtgc agtgtttgaa ggcacgtata
    14041 tcgttctgcc cgcgaagcca tttggcccgt atgaagaggg cgggaacgaa gaagagtagg
    14101 ggcgttttac ttgactgaaa aggttttcaa tctgaaaacc ttttcagggc ccaggtccga
    14161 agttaccttc ccgctctcca ggcaacatcc cgtagtgccg atcgcgcggc ctcactgata
    14221 ccacctaact gaaaaaatcc gtggataacg ccaaggtagc gttggcaggt gcattgcact
    14281 ccttgccctg tcatgcggtg atacaacgct tcaccttcat cgcaaagtgg gtcgtattca
    14341 gcggtaatga tatgtacagg tggcaagcca ttgaaatcat cgcgccaaag agggctggct
    14401 tcaggatgcc tgcgttcaat gcctggcaga tacatctcaa agccgctcag taaagtgtcg
    14461 cgtgtgatga tgtaatcgtg accattgcgt atgtagcttt cgaaatgcgc agtcgcatca
    14521 agcataggat aaattaagat gagttgtgcc ggttgccact cgccagcacg ctttaatcgc
    14581 aaagcagtta ccagcgccag atgtccgccc gcactgtcac cggcaagagt tatacgttgt
    14641 ttatcaacgc cgagtcgttc ggcgtgttgc cagactattt ctgcacctcg ttgcgcatca
    14701 tcatgggcag cggggaaagt atgttccggt gccagccgat actggacggc aataacccga
    14761 cattgcccgt aataggctaa ctggcgcagc tggttgtcgt gagttgcgaa cccgccgcta
    14821 acaaaacagc caccgtagta ataaattatc gtcgggagta atgtcggggc attcagcggc
    14881 gacactattc gtaaggtcat cccttcaagt tcgatagtct gaatatctac ccgcgtttct
    14941 gtctcacccg caaggacagc gctggcgata tagccctccc tgcgctgcgc aatactttgt
    15001 tgacgcgatg aggggcgacc cgcagcgata aacttttcga ctaactctgc aattcctttt
    15061 tccagtgcca tagtagaact tattttgctg tatgaatgta cagtcttata cacccgtttt
    15121 tgctggatgg atacagggaa attcacgggc gtgaaaaatc tggaaagcgc ctcgcaaatc
    15181 taaagacaaa cccggcagag acgtggtttt gctggagcac tatcagtaag ctagtggttt
    15241 caagggtacg tcaatgacgt acaaaaagga ttgttactct aagccaatgg cgtataatta
    15301 tgctcttcat cgaaacggaa atttttactg aagatgttca aaaactgctg accgacgatg
    15361 aattcagtcg ttttcagttt ttcctggcct tgaatcctga ctatggtgag gtaatacctg
    15421 aaactggtgg gttaagaaag gttcgatggg tgtcaggagg aaaggggaaa cgtgctggtg
    15481 tcagggttat ttactttcat caggttaaac attatgagat cagattactg cttatttatc
    15541 gtaagggtat taaagatgat ctgtctcccc aggaaaaagc aatgctccgg ttgttaaata
    15601 cgaggtggta aatggataag gcactttttg agcggttgac tcacagcatg gcccagatga
    15661 atgaaattat tgaaggaacg cgacagcctt cgcggacttt tgaagttgat gctatgaaaa
    15721 tcaaagaaat acgccgcgct tcagggttgt cacaatctaa atttgccgat cttatttctg
    15781 taagcgtaga cacgttgcgt aactgggagc aggggcgacg ttcaccgacc gggcctgcaa
    15841 aggctttgct gcgagcgatt gccaatgatc cgcaacatgt gctgcaagca cttaaccgtt
    15901 agaggcatgg ttatgccata cagaaagcac tagcattcag aactcctgtg aaagcggcat
    15961 agtggtcgcc tgcccaggag ttttgctatg acccgtaatc gtgctgaacg tcgccaccct
    16021 atgatgcgat taaaaaaact catcgtcgcg ctgatgattg acgtcaatgc tatcggatga
    16081 taccgggggg ttccggaagg aattccttgt ttcccgaact gttaccaaca aaattataac
    16141 gatgaacaag taaagaccgg ttggtggcag tactgaccgg tctgtgatgg atgattaatt
    16201 tcaccttaaa tccgggtgct gttaggatgc tgacattttt cgggtacacc attcagatta
    16261 tgcaaataaa gatccaatca aa
//
LOCUS       DAZKGS010000101        16062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKGS010000101 DAZKGS010000000
VERSION     DAZKGS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(246..1508)
                     /locus_tag="ACHOQY_003208"
     CDS             complement(246..1508)
                     /locus_tag="ACHOQY_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9679259.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1672..1756)
                     /locus_tag="ACHOQY_003209"
     tRNA            complement(1672..1756)
                     /locus_tag="ACHOQY_003209"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1720..1722),aa:Leu,seq:caa)
     gene            1952..2971
                     /gene="ahr"
                     /locus_tag="ACHOQY_003210"
     CDS             1952..2971
                     /gene="ahr"
                     /locus_tag="ACHOQY_003210"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP9679260.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            3101..4603
                     /gene="yjgR"
                     /locus_tag="ACHOQY_003211"
     CDS             3101..4603
                     /gene="yjgR"
                     /locus_tag="ACHOQY_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9679261.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4722..5804)
                     /gene="lptG"
                     /locus_tag="ACHOQY_003212"
     CDS             complement(4722..5804)
                     /gene="lptG"
                     /locus_tag="ACHOQY_003212"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9679262.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5804..6904)
                     /gene="lptF"
                     /locus_tag="ACHOQY_003213"
     CDS             complement(5804..6904)
                     /gene="lptF"
                     /locus_tag="ACHOQY_003213"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9679263.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6967..7017)
                     /gene="ytgA"
                     /locus_tag="ACHOQY_003214"
     CDS             complement(6967..7017)
                     /gene="ytgA"
                     /locus_tag="ACHOQY_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9679264.1"
                     /translation="MSIIILATISTTQENK"
     gene            7171..8682
                     /gene="pepA"
                     /locus_tag="ACHOQY_003215"
     CDS             7171..8682
                     /gene="pepA"
                     /locus_tag="ACHOQY_003215"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9679265.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9036..9479
                     /gene="holC"
                     /locus_tag="ACHOQY_003216"
     CDS             9036..9479
                     /gene="holC"
                     /locus_tag="ACHOQY_003216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9679266.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9479..12334
                     /gene="valS"
                     /locus_tag="ACHOQY_003217"
     CDS             9479..12334
                     /gene="valS"
                     /locus_tag="ACHOQY_003217"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9679267.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12388..13584)
                     /gene="yjgN"
                     /locus_tag="ACHOQY_003218"
                     /pseudo
     CDS             complement(12388..13584)
                     /gene="yjgN"
                     /locus_tag="ACHOQY_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13777..14280
                     /gene="yjgM"
                     /locus_tag="ACHOQY_003219"
     CDS             13777..14280
                     /gene="yjgM"
                     /locus_tag="ACHOQY_003219"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9679268.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14326..14742)
                     /gene="rraB"
                     /locus_tag="ACHOQY_003220"
     CDS             complement(14326..14742)
                     /gene="rraB"
                     /locus_tag="ACHOQY_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9679269.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14904..15917
                     /gene="argF"
                     /locus_tag="ACHOQY_003221"
     CDS             14904..15917
                     /gene="argF"
                     /locus_tag="ACHOQY_003221"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9679270.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            complement(15970..>16062)
                     /locus_tag="ACHOQY_003222"
     CDS             complement(15970..>16062)
                     /locus_tag="ACHOQY_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679271.1"
                     /translation="NNEQLGKLNPPENLRIAIEKFGWKKKTITA"
BASE COUNT         4124 a         4057 c         4132 g         3749 t
ORIGIN      
        1 gataaaacca caccgactaa aactgtaatg aacagagtta gtcggctggt ttctataaca
       61 aaagccatac cgtttgaaca ctttttgttt tttcactatc atctcagaat ggccattgca
      121 cctcgctcta aaataatgcc ccttggttga ggttttcccg cattttaaaa gagcatttca
      181 atttattatt atataattct ctcacacaat taccaaatta gttaatgtat aatatttcag
      241 ttacattatt tccccatgaa tttagcaaat tcataaggtg taacagagtt atttcgattg
      301 gcctcaagaa aatcagccca ccattgaacc attaatctac gttcgtctaa atgctcagag
      361 gtatgaatat atgctgcacg cacattgtta cgctctgtat gactcaactg ccgttctatc
      421 gcgtcatcgc tccataagcc tgattcacca agcgcgccac gcgccatcgt cctaaatcca
      481 tgcccacaga cctcagtttt cgtgtcatac cccatcgcac gcaaggcgct atttactgtg
      541 ttctcactca taactttggc cgcgtcgtga tcccctggaa aaagtaactc tttatcgcca
      601 ctaatctgct tgagttgctc taacaaaacc atcgcctgtc gactaagagg aacgatatgc
      661 tcctctttca tcttcatgcc acgatacgag taacgcacgc ctttaatttc ttctcgcttt
      721 gcaggtatac gccaaagaga tttatcgaag tcgaactcat cccatcgcgc gaaacgtaac
      781 tcactggaac gtacaaaagt tagtaaggaa agctcgaccg cgatccttgt cattacacga
      841 ccacgatatg cagcaagacg tgcaagaaac tcagagaagc ggctagaggg taaagctgga
      901 tagtgtcgtg ctttggttgt agataacgca ccggccatgt cactggctgg attagagtcg
      961 atgtaatcgt tctgtacggc ataacgcata atggccgtga cgcgctgttg caggcgctga
     1021 gcgacatcgt gtttgccact ggcatcaact tttttaatcg gggctaacag gtggctggtt
     1081 ttaagctgcc gaatgtcgga cgaaccgata tgaggaaaaa tataaagctc aagatagcgc
     1141 agaatgcgtg atcgatggtc ttcactccag cgcttattac tggcatgcca ttcacgagca
     1201 attgtttcga aagtgtatgc gcccgaattc tcagcctgag cttctttctg ctcggctttt
     1261 gggtcaatgc cctgcactaa gagcttttta gcctcgtcac gctttgctct tgcctgagca
     1321 agtgtcacca tgggccaaac accaaaagcg aggcgatcct cttttttgtc agaggggcgt
     1381 ctgtatttca tgcgccagta tttagaaccc ttagccgaaa cctcgagata caaaccgccg
     1441 ccatcggcca ttttgtaggt tttctctttt ggctttgctg tctcgacctg tctggcgttg
     1501 agcttcattt gggggcacat ttctaatcga agttaagatg cccccaatta tgcccccaat
     1561 agcatccgga tttcaacgga ctagctcgga cagctcagga cagaaaaacc gctacaggca
     1621 ttgattttaa agggtttgct ggattttctc ggatggtctt ggaagtacta atggtgccga
     1681 aggccggact cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg
     1741 atttcgccac ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca
     1801 cgccaccatg caagcgccag cttgcaccca cctcgctaag tgctgaaatt ttcagcatta
     1861 cactcagcga tatgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac
     1921 ttcccaaaca acaccagaga aggacccaaa aatgtcgatg ataaaaagct atgccgcaaa
     1981 agaagcgggc ggcgaactgg aagtttatga gtacgatccc ggtgagctga agccacaaga
     2041 tgttgaagtg caggtggatt actgcgggat ttgtcattcc gatctgtcga tgatcgataa
     2101 cgaatgggga ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtggt
     2161 ggcactcggg agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg
     2221 ctggacggcg cgtagctgtg gtcactgcga cacctgtatt agcggtaatc agatcaactg
     2281 cgagcaaggt gcggtgccga cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc
     2341 ggactggcaa tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct
     2401 gttgtgcggc ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag
     2461 ccgcgttggg gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc
     2521 aatgggatgc gaggtgacgg cctttagttc taatccggcg aaagagcagg aagtactggc
     2581 gatgggtacc gataaagtgg tgaatagccg cgatccgcag gcactgaaag ccctgtcggg
     2641 gcagtttgat ctcattatca atactgtgaa cgtcagcctc gactggcagc cttattttga
     2701 ggcgctgacc tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt
     2761 tccggccttt acgttaattg cgggcgaccg cagcatctct ggttctgcta ccggcacgcc
     2821 ttatgagctg cgtaagctga tgcgttttgc cgcccgcagc aaggttgcgc cgaccaccga
     2881 actgttcccg atgtcgaaaa ttaacgacgc catccagcat gtacgcgatg gtaaagcgcg
     2941 ctaccgcgtg gtcctgaaag ccgacttctg atccctttgc cgggagcctg tcgcccggca
     3001 cttaaatctc tttggaaggc gtctcgcaat ctgctgaatt tagcggtgaa gagtgcacag
     3061 ccgttgccta tactccaggc aggaaactgg aggaaatctc atgagcgaac ccctgttaat
     3121 tgcccgcacg ccggacacag aactgttttt actgccggga atggctaacc gtcacgggct
     3181 gattactggc gcaacgggga cgggtaaaac tgtcacgctg caaaaactgg cggagtctct
     3241 atcggaaatc ggcgtgccgg tgtttatggc cgatgtgaaa ggcgatctga ccggtatcgc
     3301 gcaggcagga acggcgtcgg aaaaactgct cgcaaggctt aaaaatatcg gcgtaaatga
     3361 ctggcaaccg catgctaatc cggtggtggt gtgggatatc tttggcgaga aaggccatcc
     3421 ggtgcgggcg acggtttcgg atctggggcc gctgttgctg gcgcggctgt tgaatctcaa
     3481 cgatgtgcaa tctggcgtgc tgaatatcat cttccgaatt gctgacgatc aggggctgtt
     3541 actgctcgac tttaaagatc tgcgggcgat tacccagtac atcggcgata acgccaaatc
     3601 tttccagaat cagtacggaa atatcagtag cgcatcggtt ggtgccatcc agcgcgggct
     3661 gttgtcgctg gaacagcaag gcgcagcaca cttctttggt gagccgatgc tggatatcaa
     3721 agacttgatg cgcaccgatg ccaacggtaa aggcgttatt aatatcctca gcgccgagaa
     3781 gctttatcag atgccgaaac tgtacgccgc cagcctgctg tggatgattt cagagttgta
     3841 tgaacaattg ccggaagcgg gcgatctgga gaagccaaaa ctggtgtttt tcttcgacga
     3901 agcacatctg ctgtttaacg atgcaccgca ggtactgctg gataagattg agcaggtgat
     3961 aaggcttatt cgctcaaaag gcgtgggcgt ctggttcgtt tcgcaaaacc cgtctgatat
     4021 tccggataat gtgctcggcc agctcggtaa tcgcgttcag cacgctttgc gggcttttac
     4081 gccaaaagat cagaaagcgg taaaagctgc ggcgcaaacc atgcgggcca atccggcgtt
     4141 tgataccgaa aaggcaatcc aggaactggg gaccggcgag gcgttaatct cgtttctcga
     4201 tgcaaaagga agtccttctg tggtggaacg ggcgatggtg attgcgcctt gttcgcggat
     4261 gggaccggtg acggaagatg agcgcaatgg cttgattaac cactccccgg tgtatggcaa
     4321 gtacgaggat gacgtggacc gggaatccgc ctatgagatg ttgcaaaaag gcgtacaggc
     4381 gagtattgaa cagcaaaata atcctccagc gaaagggaaa gagatagcgg tagatgacgg
     4441 cattcttggt ggattgaagg atattttgtt cggcactacc ggaccacgcg gcgggaagaa
     4501 agatggtgtg gtgcagacaa tggccaaaag cgccgctcgc caggtgacga atcagattgt
     4561 acgcgggatg ttggggagtt tgctgggagg gagaagaagg taagtcaggc gcgatctgtc
     4621 cccctcaccc taaccctctc cccaaagggg cgaggggacc gatcgaactc gtatttgact
     4681 ttatcaacaa tgtgaaaggg agctttcgct cccttgcttg gttacgattt tctcattaac
     4741 agccacaggc tgattaagaa gaagctggcg cttggcaaca gtgcgccgat aatcggcggg
     4801 atgccgtaaa ccaacgtcag cgggccgaag atctggtcca gtacgtagaa gacaaaaccg
     4861 aagctgatac cggtgaccac acgcacgccc atcggtacgc tacgcagtgg gccaaagatg
     4921 aacgacagcg ccatcagcat catcaccgcc acggatagcg gctggaagat tttgctccac
     4981 atattgagct gataacgtcc ggcatcctga ccgctcgact tcagatactt cacgtagttg
     5041 tgcaaaccgc tgattgagag tgcatccggg tccagcgcca ccacgcccag tttgtctggc
     5101 gtgaggttgg ttttccaggt gccgctcacc gtctgcgaac cggtgatctg tttcggattg
     5161 gtcagatcag attcatcaac ctgcgacaga cgccagactt tatgttccgg gtcaaacttc
     5221 gcagtagcgg catagcgtac ggattgcaga cgacgatttt cgttaaaggc ataaatgctg
     5281 atgccaccta actcttcgtc acctttaacc cgctcaatgt agacgaagtt gttgccatct
     5341 ttcgcccata agccttgctg ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg
     5401 tagttacgcg ccatctgctc gccctgcggc gcgacccatt cgccaatcgc catcgtcagc
     5461 aagaccagcg gaatggcggt tttcatcacc gacagcgcca cctgcatacg ggtaaaacca
     5521 gaagcctgca tcaccaccag ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc
     5581 ccaagcagag ccgccatcgg gaagaagatt tgcacatctt tcggcacgct cagcaaggta
     5641 tacattcctg cgcctaacgc gtcgtaactc ccctgcccgg cttttttcaa ctgatcgaca
     5701 aatttgataa tgcccgacag cgacaccagc atgaacagcg tcatcatgat ggtggtgaaa
     5761 atagttttac cgatatagcg gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac
     5821 gcgaaaaact ggcgcgcagg cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg
     5881 ccaaagccag ataaatcagg ttaacggtcc acatccacag cgtcgggtcc agcttacctt
     5941 taccgccgtt cgatttcagg gaggtctgga tcaggaagaa aagtagatac agcagcatgg
     6001 ctggcagcat cgacagtacg cgtccctgac gtgggttaac cacgctcagc ggtacgacca
     6061 taagtgccat cataaacacg gtgaatacca acgtgatacg ccagttcagt tctgcgcgag
     6121 cacgatcggt gtcagtgttc cacaatgtgc acatgtccat ctggtcggta tcgttcgggt
     6181 cgagcgccac cgcctggtga ccaatgatcg cctgataatc ctggaaatcc gtaatgcgga
     6241 aatcacgtaa cagtgcagtg ccttcgaagc gcgttccctg gttgagagtg acgacctggg
     6301 agccgtcgcg cagctgggtt aaatgtccgg aatcggccac caccacagaa ggacgtgcat
     6361 taccttttgg tcgaatttgt gcgaggaaca catctttgaa atcgctgccg tcaacgcttt
     6421 cgatgaacag caccgagctg ccattagtcg cttgctggaa ttgcccttgc gccagcgccg
     6481 ccatgccagg gttcgctttc gcttctgcta acacttcatc ctgatgacgc gatgaccacg
     6541 gtcccgccca catcacgtta accgccgcga cgattgccgt gaataccgca aggatcattg
     6601 ccgctttcac cagaaccgct ttgctcaggc cgcaggcatg cattaccgta atttcacttt
     6661 cggtatacag tttgcccagc gtcatcagca gcccgaggaa caggcttaat ggcaggataa
     6721 gctgcgccat ttccggcacg cccaacccga gaagggagag caccagattc gccggaatat
     6781 cgccgtcaac cgctgcgccg aggatcctca ctaacttttg acagaagaag atcaaaagca
     6841 agatgaagag tatcgccagc tggcttttga gcgtctcccg caccagatat cttatgatta
     6901 tcactttaaa tacgcccgta aaaactcgtc ttttgcagga ttttagcttg tttcatggct
     6961 taaacgtcat ttattctctt gagtcgtcga aatcgtcgct aagataatta tactcaacgg
     7021 attcacctct cagattttgt tctgacgtgc caatgccgta ataacgttaa gattaacacg
     7081 aagtcatcgc aacagcggac atgagttacg aaagcttgca attctatctg tagccaccgc
     7141 cgttgtcttt aagattcagg agcgtagtgc atggagttta gtgtaaaaag cggtagcccg
     7201 gagaaacagc ggagtgcctg catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg
     7261 attgcagaac agctcgataa aatcagcgat gggtacatca gcgccctgct acgtcggggc
     7321 gaactggaag gaaaaccggg gcagacattg ttgctgcacc atgttccgaa tgtactttcc
     7381 gagcgaattc tccttattgg ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag
     7441 caggttattc agaaaaccat taatacgctg aatgatactg gctcaatgga agcggtctgc
     7501 tttctgactg aactgcacgt taaaggccgt aacaactact ggaaagtgcg tcaggctgtc
     7561 gagacggcaa aagaaacgct ctacagtttc gatcagctga aaacgaacaa gagcgaaccg
     7621 cgtcgtccgc tgcgtaaaat ggtgttcaac gtgccgaccc gccgtgaact gaccagcggt
     7681 gagcgcgcga tccagcacgg tctggcgatt gccgccggga ttaaagcagc aaaggatctt
     7741 ggcaatatgc cgccgaatat ctgtaacgcc gcttacctcg cttcacaagc gcgccagctg
     7801 gctgacagct acagcaagaa tgttatcacc cgcgttatcg gcgaacagca gatgaaagag
     7861 ctggggatgc attcctatct ggcggtcggt cagggttcgc agaacgaatc gctgatgtcg
     7921 gtgattgagt acaaaggcaa cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa
     7981 ggtttaacct tcgactccgg cggtatctcg atcaagcctt cagaaggcat ggatgagatg
     8041 aagtacgata tgtgcggtgc ggcagcggtt tacggtgtga tgcgtatggt cgcggagcta
     8101 caactgccga ttaacgttat cggcgtgttg gcaggctgcg aaaacatgcc tggcggacga
     8161 gcctatcgtc cgggcgatgt gttaaccacc atgtccggtc aaaccgttga agtgctgaat
     8221 actgacgctg aaggccgcct ggtactgtgc gacgtgttaa cttacgttga gcgttttgag
     8281 ccggaagcgg tgattgacgt ggcgacgctg accggtgcct gcgtgatcgc gctgggtcat
     8341 cacattaccg gtctgatggc gaaccataat ccgctggccc atgaactgat tgccgcgtct
     8401 gaacaatccg gtgaccgcgc atggcgctta ccgctgggtg acgagtatca ggaacagctg
     8461 gagtccaatt ttgccgatat ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca
     8521 ggttgcttcc tgtcgcgctt tacccgtaag tacaactggg cgcacctgga tatcgccgga
     8581 accgcctggc gttctggtaa agcaaaaggc gcaaccggtc gtccggtagc gttgctggca
     8641 cagttcctgt tgaaccgcgc tgggtttaac ggcgaagagt aattgcgtca ggcaaggctg
     8701 ttattgccgg atgcggcgtg aacgccttat ccgacctaca cagcactgaa ctcgtaggcc
     8761 tgataagacg cgcaagcgtc gcatcaggca atgctgttat tgtcggatgc ggcatgaacg
     8821 ccttatccga cctacacagc accgaactcg taggcctgat aagacacaac agcgtcgcat
     8881 caggcaatgc tgttattgtc ggatgcggcg tgagcgcctt atccgaccta cacacagcac
     8941 tgaactcgta ggcctgataa gacacaacag cgtcgcatca ggcgctgcgg tgtatacctg
     9001 atgcgtattt aaatccacca caagaagccc catttatgaa aaacgcgacg ttctaccttc
     9061 tggacaatga caccaccgtc gatggcttaa gcgccgttga gcaactggtg tgtgaaattg
     9121 ccgcagaacg ttggcgcagc ggtaagcgcg tgctcatcgc ctgtgaagat gaaaagcagg
     9181 cttaccggct ggatgaagcc ctgtgggcgc gtccggcaga aagctttgtt ccgcataatt
     9241 tagcgggaga aggaccgcgt ggcggtgcgc cggtggagat cgcctggccg caaaaacgca
     9301 gcagcagccc gcgcgatata ttgattagtc tgcgaacatg ctttgcagat tttgccaccg
     9361 ctttcacaga agtggtagac ttcgttcctt atgaagattc tctgaaacaa ctggcgcgcg
     9421 aacgctataa agcctaccgc gtggctggtt tcaacctgaa tacggcaacc tggaaataat
     9481 ggaaaagaca tataacccac aagatatcga acagccgctt tacgagcact gggaaaagca
     9541 gggctacttt aagcctaatg gcgatgaaag ccaggaaagt ttctgcatca tgatcccgcc
     9601 gccgaacgtc accggcagtt tgcatatggg tcacgccttc cagcaaacca tcatggatac
     9661 catgatccgc tatcagcgca tgcagggcaa aaacaccctg tggcaggtcg gtactgacca
     9721 cgccgggatc gctacccaga tggtcgttga gcgcaagatt gccgcagaag aaggtaaaac
     9781 ccgtcacgac tacggccgcg aagctttcat cgacaaaatc tgggaatgga aagcggaatc
     9841 cggcggcacg attacccgtc agatgcgccg tctcggcaac tccgtcgact gggagcgtga
     9901 acgcttcacg atggacgaag gcctgtccaa tgcggtgaaa gaagttttcg ttcgtctgta
     9961 taaagaagac ctgatttacc gtggcaaacg cctggtaaac tgggacccga aactgcgcac
    10021 cgctatctct gacctggaag tggaaaaccg cgaatcgaaa ggttcgatgt ggcacatccg
    10081 ctatccgctg gctgacggtg cgaaaaccgc agacggtaaa gattacctgg tggttgcgac
    10141 tacccgtccg gaaaccctgc tgggcgatac cggtgtagcc gttaacccgg aagatccgcg
    10201 ttacaaagac ctgattggca aatatgtcat tctaccgctg gttaaccgtc gtattccgat
    10261 cgttggcgac gaacacgccg acatggaaaa aggcaccggc tgcgtgaaga tcaccccggc
    10321 gcacgacttt aacgactatg aagtcggtaa acgtcacgcc ctgccgatga tcaacatcct
    10381 gacctttgac ggcgatatcc gtgaaagcgc ccaggtgttc gataccaaag gtaacgaatc
    10441 tgacgtttat tccagcgaga tcccggcaga attccagaaa ctggagcgtt ttgccgcacg
    10501 taaagcagtc gttgctgcgg ttgacgcact cggcctgctg gaagaaatta aaccgcacga
    10561 cctgaccgtt ccttacggcg accgtggcgg cgtagttatc gaaccaatgc tgaccgacca
    10621 gtggtacgtg cgtgccgatg tactggcgaa accggcggtt gaagcggttg agaacggcga
    10681 cattcagttc gtaccgaagc agtacgaaaa catgtatttc tcctggatgc gcgatattca
    10741 ggactggtgt atctctcgtc agctgtggtg gggtcaccgt atcccggcat ggtatgacga
    10801 agcgggtaat gtttatgttg gccgcaacga agaagaagtg cgcaaggaaa ataacctcgg
    10861 tgccgacgtt gccctgcgtc aggacgaaga cgttctcgac acctggttct cttctgcgct
    10921 gtggaccttc tctacccttg gctggccgga aaacaccgac gccctgcgtc agttccaccc
    10981 aaccagcgtg atggtatccg gcttcgacat catcttcttc tggattgccc gcatgatcat
    11041 gatgaccatg cacttcatca aagatgaaaa tggcaaaccg caggtgccgt tccacactgt
    11101 ttacatgacc ggtctgattc gtgacgacga aggccagaaa atgtccaaat ccaagggtaa
    11161 cgttatcgac ccgctggata tggttgacgg tatttcgctg ccagaactgc tggaaaaacg
    11221 taccggcaat atgatgcagc cgcagctggc ggacaaaatc cgtaagcgca ccgagaaaca
    11281 gttcccgaac ggcattgagc cgcacggcac cgatgccctg cgcttcaccc tggcggcgct
    11341 ggcgtctacc ggtcgtgaca tcaactggga tatgaagcgc ctggaaggtt accgtaactt
    11401 ctgtaacaag ctgtggaacg ccagccgctt tgtgctgatg aacacagaag gtcaggattg
    11461 cggctttaac ggcggcgaaa tgacactgtc gctggcggac cgctggattc tggcagagtt
    11521 caaccagacc atcaaagcgt accgcgaagc gctggacagc ttccgcttcg atatcgctgc
    11581 aggcattctg tatgagttca cctggaacca gttctgtgac tggtatctgg agctgaccaa
    11641 gccggtaatg aacggtggca ccgaagccga actgcgcggt actcgccata cgctggtgac
    11701 tgtactggaa ggtctgctgc gcctcgcgca tccgatcatt ccgttcatca ccgaaaccat
    11761 ctggcagcgt gtgaaagtac tttgcggtat cactgccgac accatcatgc tgcagccgtt
    11821 cccgcagtac gatgcatctc aggttgatga agccgcactg gccgacaccg aatggctgaa
    11881 acaggcgatc gttgcggtac gtaacatccg tgcagaaatg aacatcgcgc cgggcaaacc
    11941 gctggagctg ctgctgcgtg gttgcagcgc ggatgcagaa cgtcgcgtaa atgaaaaccg
    12001 tggcttcctg caaaccctgg cgcgtctgga aagcatcacc gtgctgcctg ccgatgacaa
    12061 aggtccggtt tccgttacga agatcatcga cggcgcagag ctgctgatcc cgatggctgg
    12121 cctcatcaac aaagaagatg agctggcgcg tctggcgaaa gaagtggcga agatcgaagg
    12181 cgaaatcagc cgtatcgaga acaagctggc gaatgaaggc tttgtcgccc gcgcaccgga
    12241 agcggtcatc gcgaaagagc gtgagaagct ggaaggctat gcggaagcga aagcgaagct
    12301 gattgaacag caggctgtta tcgccgcgct gtaatcaaca ccgtgatgaa aacgaaggcc
    12361 ggagaatgct ccggcctttt ttatctctta cagaaatggt aaagaaggca taattccccg
    12421 tgaaatactg gctaagaagc ctttatctgg ttgttcttct ttattgatta aaggaaggtc
    12481 atccaaatcg cccagcaaat acgtattttt tgcctgccag tcaatcatcc atattttcag
    12541 cagtggataa gccagaccgc ccgtaatccc ggatatcact accaacgcac acatgcgata
    12601 aagcataccg tggtaagtta aagttgagcg aaaacgaata cgcccatcat tcagtgacag
    12661 gttgctcata aaatgatttc gcaacgacac cgttaaataa ctggtgctaa cagcaatccc
    12721 aaaataatag attaactgcg cgattatcat tttacgttgc atttccataa attgctgtaa
    12781 attctcaata tcatcatttg catacacaga actatcatac gcatttaata tttgatcgaa
    12841 gataatataa ccagcaacag cgagaaaagg taataaagct aaaaatgcga ggatggcata
    12901 ttttatacaa tacgcagtat ctaatttcac cttgaaacga tgtataccga aactggtatt
    12961 gctccagaga aaactcatta ccaggctata taaagtacca ttaaaaatac caatggaaac
    13021 aataccaact attgccatca gaaccacaga tataataaca ctacttgaac tattggcatg
    13081 tagcatcttt gtcgagataa agaaaacagt ccccatccca atggccatta aaatcggcaa
    13141 gaaaaaggtt actcaccaga accctttcat agagcaatta aaactaaatc ttacgccgtt
    13201 gagactagtc atcaaggcct gataacgcag tccttttgct gccataaata taagcaaaac
    13261 caacagtgac aaagtcaaaa cacagccaat aagcggcata tctgctgaca ctgtcattaa
    13321 gattgcgaaa tagaaaaaaa caaaaacaag acaactaaca aaaacattcc caccggtaat
    13381 cccataagaa aatcgttgtc cgttaacctc catattagca tagagataac gcttacattt
    13441 cattaatgcc catggtaaat agatacctag cgttataatc gttaacaaca cattcaccac
    13501 acaaataaga aaatatctct cacctgtacc atgaaaaaca aacgaatggg acggaacatc
    13561 tatttcatta ataacttgag ccataaaaat tccctgaatt aaaaaatcca ttgatataag
    13621 ttatagatac agacagtaaa atgttctgcc acgttacaat actcttatca gtaataggct
    13681 ggcaaaaact ttattgcttt ataagatgct tgcgcacgta agaagagagt ggtattaaac
    13741 aaatgtataa ttactttatt tttattgggt taaactatga ataacattgc gccgcaatca
    13801 cctgtaatgc gtcgcctgac gctgcaggat aatcctgcta ttgcccgcgt cattcgtcag
    13861 gtatccgccg aatacggtct taccgctgat aaaggctaca ccgtcgccga tccgaatctt
    13921 gacgagctgt atcaagtgta tagtcagccg ggtcatgcat attgggtggt tgagtacgag
    13981 ggtgaagtgg tcggcggcgg tgggattgcg ccattagcag gtagtgaatc ggacatttgc
    14041 gaactgcaaa agatgtattt tcttccggct attcgcggta aagggctggc gaaaaaactg
    14101 gccttaatgg cgatggagca ggcacgagag atgggcttca aacgctgcta tctggaaacg
    14161 accgcttttt taaaggaagc cattgcgctt tatgagcatt tgggctttga gcatatcgac
    14221 tatgcgcttg gctgcacggg ccatgtcgat tgtgaagtgc ggatgctacg cgaactctaa
    14281 aaaaatgccc gctagtgtgt gctatcgggc attttaatca attaattagt ggcgaacccc
    14341 gtcatcgtct tcatcgacaa aatcttcatc gtcgccgtct tcgccgttgg gatcttcaaa
    14401 gtaagtgccc caaccgtcgt actcaacgtc aaatttctcc gccagcgtca tcagttgttc
    14461 aacctgggca tcgatcagat cggcattcaa cgcgcactcg ctgaggatgt cgcagcaaat
    14521 cacgatatca ccatcttcaa cttccagctc ttctggatcg gtcacttcgt aaccgagttt
    14581 aaacgcttca actgctgctt tttccagggt ttccagatcg tctgcggaaa gatggtgttc
    14641 gatggtatac agcgcgtccg gatcgctgcc atcttccagt aattcttcaa taatcaaacg
    14701 tgtttcttca cgctgttctt ccagttgttc cgggtttgcc atggctcatt cctcttaatg
    14761 tcctgccgat actcttattg tcacacacca ctgacattgc ctccaccttt gtgacaaaga
    14821 tttatgcttt agacttgcaa atgaataatc atccatataa attgaatttt aattcattga
    14881 ggtgttagcc acaggaggga tctatgtccg ggttttatca taagcatttc ctgaaattac
    14941 tcgatttcac gccagctgaa ctcaacagcc tgctgcagtt agccgcgaag ctgaaagccg
    15001 ataagaaaag cggcaaagaa gaagccaaac tcactggtaa aaacatcgcg ctcatcttcg
    15061 aaaaagactc gacccgtacc cgatgctctt tcgaagttgc cgcatatgac cagggtgctc
    15121 acgttactta tctcggccca agcggcagtc agattggtca taaagagtcg attaaagaca
    15181 ctgcccgcgt gctgggtcgc atgtatgacg gtattcagta tcgcggctat ggtcaggaga
    15241 ttgtcgaaac actggcggaa tacgctggcg tgccggtatg gaacggcctg accaatgagt
    15301 ttcaccccac gcagttgctg gcggatctcc tcaccatgca ggagcattta cccggtaaag
    15361 cattcaacga aatgacgctg gtctatgcag gtgacgcacg taacaacatg ggcaattcga
    15421 tgctcgaagc tgcggcgctt accggtctgg atttgcgtct ggtcgcgcca caggcatgct
    15481 ggccggaagc tgcgctggtt acggaatgcc gcgctctggc acagcaaaat ggcgggaata
    15541 ttacgctgac ggaagatgta gctaaaggag ttgaaggtgc tgactttatc tataccgatg
    15601 tatgggtgtc gatgggtgaa gcaaaagaga aatgggctga acgcattgca ttgctgcgtg
    15661 attatcaagt gaacagcaag atgatgcagt tgaccggtaa cccggaggtc aaattcctcc
    15721 actgcctgcc cgcgtttcat gacgaccaaa cgacgcttgg caagaaaatg gcggaagagt
    15781 ttggcctaca tggcggaatg gaagtgactg atgaggtctt cgaatctgcc gccagcattg
    15841 tatttgatca ggcggaaaac cgcatgcata ctatcaaagc ggtgatggtc gcgacgctca
    15901 gtaaattaaa taattaatat ctctatcaag atgccgggaa atacaaaaac atcccggcat
    15961 cacattattt tatgcagtga tagttttttt cttccagcca aatttttcta ttgctatacg
    16021 taaattttcc ggaggattta attttcccaa ctgttcatta tt
//
LOCUS       DAZKGS010000102        15803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKGS010000102 DAZKGS010000000
VERSION     DAZKGS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..1631)
                     /gene="putP"
                     /locus_tag="ACHOQY_003223"
     CDS             complement(123..1631)
                     /gene="putP"
                     /locus_tag="ACHOQY_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9679272.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(1790..1999)
                     /locus_tag="ACHOQY_003224"
     CDS             complement(1790..1999)
                     /locus_tag="ACHOQY_003224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679273.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            2054..6016
                     /gene="putA"
                     /locus_tag="ACHOQY_003225"
     CDS             2054..6016
                     /gene="putA"
                     /locus_tag="ACHOQY_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9679274.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6056..6694)
                     /gene="rutR"
                     /locus_tag="ACHOQY_003226"
     CDS             complement(6056..6694)
                     /gene="rutR"
                     /locus_tag="ACHOQY_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9679275.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6982..8073
                     /gene="rutA"
                     /locus_tag="ACHOQY_003227"
     CDS             6982..8073
                     /gene="rutA"
                     /locus_tag="ACHOQY_003227"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9679276.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            8073..8765
                     /gene="rutB"
                     /locus_tag="ACHOQY_003228"
     CDS             8073..8765
                     /gene="rutB"
                     /locus_tag="ACHOQY_003228"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9679277.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            8777..9163
                     /gene="rutC"
                     /locus_tag="ACHOQY_003229"
     CDS             8777..9163
                     /gene="rutC"
                     /locus_tag="ACHOQY_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9679278.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            9171..9971
                     /gene="rutD"
                     /locus_tag="ACHOQY_003230"
     CDS             9171..9971
                     /gene="rutD"
                     /locus_tag="ACHOQY_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9679279.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            9981..10571
                     /gene="rutE"
                     /locus_tag="ACHOQY_003231"
     CDS             9981..10571
                     /gene="rutE"
                     /locus_tag="ACHOQY_003231"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9679280.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            10582..11076
                     /gene="rutF"
                     /locus_tag="ACHOQY_003232"
     CDS             10582..11076
                     /gene="rutF"
                     /locus_tag="ACHOQY_003232"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9679281.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            11097..12425
                     /gene="rutG"
                     /locus_tag="ACHOQY_003233"
     CDS             11097..12425
                     /gene="rutG"
                     /locus_tag="ACHOQY_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9679282.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(12508..12681)
                     /gene="ymdF"
                     /locus_tag="ACHOQY_003234"
     CDS             complement(12508..12681)
                     /gene="ymdF"
                     /locus_tag="ACHOQY_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9679283.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            13054..13650
                     /gene="wrbA"
                     /locus_tag="ACHOQY_003235"
     CDS             13054..13650
                     /gene="wrbA"
                     /locus_tag="ACHOQY_003235"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9679284.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGSGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            13671..13898
                     /gene="yccJ"
                     /locus_tag="ACHOQY_003236"
     CDS             13671..13898
                     /gene="yccJ"
                     /locus_tag="ACHOQY_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9679285.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(13936..15177)
                     /gene="agp"
                     /locus_tag="ACHOQY_003237"
     CDS             complement(13936..15177)
                     /gene="agp"
                     /locus_tag="ACHOQY_003237"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9679286.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15469..>15803)
                     /locus_tag="ACHOQY_003238"
     CDS             complement(15469..>15803)
                     /locus_tag="ACHOQY_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679287.1"
                     /translation="LHLPEFIFNDNKARLVLDTDPEQKVNEVKNFLTALSDSICSLFI
                     VNGNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQID
                     FLTRYAIY"
BASE COUNT         3644 a         4218 c         4206 g         3735 t
ORIGIN      
        1 ggggccgcgg cgattggccg ccccctgcgc gctccctgca ccattcaatt tatatggcga
       61 ggcacattaa gggagcgcaa cccgctgctg aagccaaaca aaaccgccgc aggctaagtc
      121 ccttagcttt cctgcaaccg tgacggcgga gccgaatgat agtgcgcatc ggcctcggca
      181 aagcgttttt gcatcgccgc tgacggcgct ttacccagca aactaaacac tacaatcccg
      241 atactgccga agatgaagcc cggaataatt tcgtagagtc ccagccagcc gaactgcttc
      301 cagacgataa ccgtcagcgc accgatgatc atccccgcca gcgcaccgtt gcgcgtcatg
      361 cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg ccgcgccaaa gcctgcccac
      421 gcgtagctca ctaagcccag cacgcggttt tccgggtttg ccgccagcgc aatcgccacc
      481 agcgccacca ccagcaccat cacacgtcct acccacacca gctctttctg gctggcctgt
      541 ttacgcagaa acgctttgta taaatcttcg gtaattgcac tggaacatac cagcagctgg
      601 caacttaagg ttgacattac cgccgccaga atcgccgaca gcagaatccc ggcaatccac
      661 gggttaaaca gaatttgcgc cagttcgata aacacgcgct cggcgttctg gtttaccgcg
      721 cctgctaccg ccgggtgctc attaaagtaa gcaatcccaa agaagccaac agccaccgcc
      781 cctgccaggc agaggatcat ccaggtcata ctgatacgac gcgcatggac aatgctgtgg
      841 tgagagtctg ccgccataaa acgcgccagg atgtgcggct gcccgaagta acccagcccc
      901 caacccatca gtgagataat ggcgacaaag ttcagccctt tgagcatatc aacgttttcg
      961 atgctctttt gtttgatcac ttccagcgag tcaccaaagc caccgacact gataatgacg
     1021 ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg cctgtacagt gtcagtccag
     1081 ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg tcgccgcagc tcccgcccac
     1141 agagccgttt cgtagctcat gccaaaggta ctttcaaaca gacgcgcgcc tgccacaatg
     1201 cccgaagcgc aataaatggt gaagaacagc aaaatcacca gcgcggagat aatgcgcaaa
     1261 atgcggcttt tatcttcaaa gcgcccggtg aaataatccg gcaatgttaa ggcgttattg
     1321 ttgtattcgg tatgcacacg caaccgcccc gccaccagct tccagttgat ccacgcgcct
     1381 aatgtcaggc caatggcgat ccaactttcg gaaatcccgg aaagaaaaac cgcgcccggc
     1441 aaccccatta acagccagcc gctcatatcc gacgcacccg ccgataatgc cgtcacgaat
     1501 ggcccaaggc tacgaccgcc cagaatatag tcgtcaaagt ttttcgttga tcgccaggcg
     1561 ataaacccaa tcaatatcat gccaaagata tagacacaaa atgtcaccaa catcggtgtg
     1621 ctaatagcca tctaaagtct ccaaaaaatt attatcggca atgtcgaaac ttgccgttat
     1681 atctgccacc ggaacggggt aacagagttt atgttttacc agggcgaccg tatcctgccg
     1741 gaagcgctgg ttattcacaa tcgatttaac acaccattta cattaaattt tagtgctcag
     1801 cgacactatt tttcatcagg ttgcactctc tcacattttt tgcggttgca cctttcaaaa
     1861 atgttaactg ccgcagagaa aaagtctgag ttattttttt aatccctgtc atatcgattt
     1921 cttttattaa catttcattc atttttaagc ttgctacgca tgtcacattt aacatggttg
     1981 cacaaagttg caacatcatg gatatttcac gataacgtta agttgcacct ttcagaacaa
     2041 caggagtaat ggcatgggaa ccaccaccat gggggttaag ctggacgacg cgacgcgtga
     2101 gcgtattaag tctgccgcga cacgtatcga tcgcacgcca cactggttaa ttaagcaggc
     2161 gattttttct tatctcgaac aactggaaaa cagcgatact ctgccggagc tacctgcgct
     2221 gctttctggc gctgccaatg agagcgatga agcaccgact ccggcagagg aaccacacca
     2281 accattcctc gactttgccg agcaaatttt gcccctgtcg gtttcccgcg ccgcgatcac
     2341 agcggcctat cgccgcccgg aaaccgaagc ggtttctatg ctgctggaac aagcccgcct
     2401 gccgcagcca gttgctgaac aggcgcacaa actggcgtat caactggccg ataaactgcg
     2461 taatcaaaaa aacgccagtg gtcgcgcagg tatggttcag gggttattgc aggagttttc
     2521 gttgtcatcg caggaaggcg tggcgctgat gtgtctggcg gaagcgttgt tgcgtattcc
     2581 cgacaaagcc acccgtgatg cgttaattcg cgacaaaatc agcaacggta actggcaatc
     2641 acacattggt cgtagcccgt ctttgtttgt taatgccgcc acctgggggc tgctgtttac
     2701 tggcaaactg gtttccaccc ataacgaagc cagcctctcc cgctcgctga accgcattat
     2761 cggtaaaagc ggtgaaccgc tgatccgcaa aggtgtggat atggcgatgc gcctgatggg
     2821 cgagcagttc gtcactggcg aaaccatcgc ggaagcgtta gccaatgccc gcaagctgga
     2881 agagaaaggt ttccgttact cttacgatat gcttggcgaa gctgcactga ctgccgcaga
     2941 tgcacaagcg tatatggttt cctatcagca ggcgattcac gccattggta aagcgtccaa
     3001 tggtcgtggc atctatgaag gtccgggcat ttcaatcaag ctgtcggcgc tgcatccgcg
     3061 ttacagccgc gcccaatatg accgggtaat ggaagagctt tacccacgtc tgaaatcact
     3121 caccctgctg gcgcgtcagt acgatattgg tatcaacatt gacgccgaag aggccgatcg
     3181 tctggagatc tccctcgatc tgctggaaaa actctgtttc gagcctgagc tggcaggctg
     3241 gaacggtatc ggttttgtta ttcaggctta tcaaaaacgc tgcccactgg tgattgatta
     3301 cctgattgat ctcgccaccc gcagccgtcg ccgtctgatg attcgcctgg tgaaaggcgc
     3361 gtactgggat agtgaaatca agcgtgcgca gatggacggc cttgaaggct atccggttta
     3421 tacccgcaag gtgtataccg acgtttcata tctcgcctgt gcgaaaaagc tgctggcggt
     3481 gccgaatcta atctacccgc agttcgcgac gcacaacgcc catacgctgg cagcgattta
     3541 tcaactggca ggtcagaact actatccggg tcagtacgag ttccagtgcc tgcatggtat
     3601 gggcgagcca ctgtatgagc aggtcaccgg gaaagttgcc gacggcaaac ttaaccgtcc
     3661 gtgtcgtatt tatgctccgg tcggtaccca tgaaacgctg ttggcgtatc tggtgcgtcg
     3721 cctgctggaa aacggtgcta acacctcgtt tgttaaccgt attgccgaca cctctttgcc
     3781 actggatgaa ctggtcgccg atccggtcac tgctgtagaa aaactggcgc aacaggaagg
     3841 gcaaactgga ttaccgcatc cgaaaattcc cctgccgcgc gatctttacg gtcacgggcg
     3901 cgacaactcg gcagggctgg atctcgctaa cgaacaccgg ctggcctcgc tctcttctgc
     3961 cctgctcaat agtgcactgc aaaaatggca ggccttgcca atgctggaac aaccggtagc
     4021 ggcaggtgag atgtcgcccg ttattaaccc tgcggaaccg aaagatattg tgggctttgt
     4081 gcgtgaagcc acgccgcgtg aggtagaaca ggcgctggaa agtgcggtta ataacgcgcc
     4141 aatctggttt gccactcctc cggttgaacg cgcggcgatt ttgcatcgcg ctgccgtgct
     4201 aatggaaagc cagatgcagc aactgattgg tattctggtg cgtgaagccg gaaaaacctt
     4261 cagtaacgcc attgccgaag tgcgcgaagc ggtcgatttt ctccactact atgccggaca
     4321 ggtgcgggat gatttcgcta acgaaaccca ccgtccatta gggcctgtgg tgtgtatcag
     4381 tccgtggaac ttcccgctgg ctattttcac cgggcagatt gctgccgcac tggcggcagg
     4441 taacagcgtg ctggcaaaac cggcagaaca aacgccgctg attgccgcgc aagggatcgc
     4501 cattttgctg gaagcgggtg taccgccagg cgtggtgcag ttgctgccag gtcaggggga
     4561 aaccgtgggc gcgcaactga cgggtgatga tcgcgtacgc ggggtgatgt ttaccggttc
     4621 aaccgaagtc gctacgttac tgcagcgcaa tatcgccagc cgcctggacg ctcagggtcg
     4681 ccctattccg ctcatcgctg aaaccggcgg catgaacgcg atgattgtcg attcttcagc
     4741 actgaccgaa caggtcgtcg tggatgtact ggcctcggcg ttcgacagtg cgggtcagcg
     4801 ttgttcggcg ctgcgcgtgc tgtgcctgca agatgagatt gccgaccaca cgttgaaaat
     4861 gctgcgcggc gcaatggccg aatgccggat gggtaatccg ggtcgcctga ccaccgatat
     4921 cggtccggtg attgatagcg aagcgaaagc caatattgag cgccatattc agaccatgcg
     4981 tagcaaaggc cgtccggtgt tccaggcggt gcgggaaaac agcgaagatg cccgtgaatg
     5041 gcaaagcggc acctttgtcg ccccgacgct gatcgaactg gatgactttg ccgaattgca
     5101 aaaagaggtc tttggtccgg tgctgcatgt ggtgcgttac aaccgtaacc agctaccaga
     5161 gctgatcgag cagattaacg cttccggcta tggtctgacg cttggtgtcc atacgcgtat
     5221 tgatgaaacc atcgcccagg tcactggctc ggcccatgtt ggtaacctgt atgttaaccg
     5281 taatatggtg ggcgcagtgg tgggtgtgca gccattcggc ggcgaagggt tgtccggtac
     5341 cgggccgaaa gcaggcggtc cgctctatct ctaccgtctg ctggcgaatc gcccggaaag
     5401 tgcgctggca gtgacgctcg cgcgtcagga tgcagagtat ccggtcgatg cacagttgaa
     5461 agccgcattg actcagccgc taaatgcact gcgggaatgg gcagcaaatc gtccagaatt
     5521 gcaggcgtta tgtacgcaat atggcgagct ggcgcaggca ggaacacaac gattgctgcc
     5581 ggggccgacg ggtgaacgca acacctggac gctgctgccg cgtgagcgcg tgttgtgtat
     5641 tgccgatgat gagcaggatg cgctgactca gctcgccgcc gtgctggcgg tgggcagcca
     5701 ggtactgtgg ccggatgacg cgctgcatcg tcagctagta aaggcattgc catcggcagt
     5761 cagcgaacgt attcaactgg cgaaagcgga aaatataacc gctcaaccct ttgatgcggt
     5821 gatcttccac ggtgattcgg atcagcttcg cgcattgtgt gaagccgttg ctgcgcggga
     5881 tggcgcaatt gtttcggtgc agggttttgc ccgtggcgaa agcaatatcc ttctggaacg
     5941 gctgtatatc gaacgttcgc tgagtgtgaa taccgctgcc gctggcggta acgccagttt
     6001 aatgactata ggttaaacag gtagccggag gatgttacaa cctcctccgg catctttaac
     6061 gtggtcgaat cccctcaata ataatccgct gcacgttttc aaccgtttga ttgaaaaata
     6121 tctcatcgcg caacgtcgcg cctgtcaccg cctccacctg aggggcgaaa tcggcgtaat
     6181 gttgagtgga agcccaaatc atgaaaatca aatgctgcgg atcaatcggc gcgagtttgc
     6241 cgcttttgac ccaaccggca atcagcgccg atttctcatc aattaatgcc ttcaaatcgc
     6301 ccgtcagttc atccattaac agcggcgcgc ctgccagcat ctccatacag aacaggcgcg
     6361 aagcctgcgg ataatcgcgg gagacttcca gcttcagacg gatgtactct tttatcgccg
     6421 ccagcggagc gaaatcttca cgaaacgctt ttaacggtgc cagccagata tcgagaatct
     6481 gccgcagcac ggcaatatac agcgcctctt ttgacggaaa gtaatacagc agattggttt
     6541 ttgaaacacc cgccaactct gcgatctgct ccagccttgt gccgtgaaaa ccgaattgtg
     6601 aaaaagtgtc cagtgctgcg ctaagaatcg cttttttctt cgcgcttact gcgcgcgaac
     6661 gtttacccgt tgttttcact gcgccttgcg tcatgcgctc tcccctcttg tagattggca
     6721 acaggatagc aaaacccccg cccggcctcg accttctgca ctctcatcgc gcacagatgc
     6781 atgttttatg tgcaactgtt ttgaccgttt agtccacttt ttaccagata ttttagacaa
     6841 cacaatgtta acacattaat aacattcact ttttaaaaac tggcatccgc tttgcaaaca
     6901 agccattacg cggctgaaaa ggatatgcag gatgcagcgc cccgtctgac tttcacttta
     6961 cgagacgaag agaggttgat gatgaaaatt ggcgtattcg tacctattgg caacaacggc
     7021 tggctcattt cgacccacgc gccgcagtac atgccgacct ttgaactgaa taaagccatt
     7081 gtgcaaaaag cggagcacta ccatttcgat ttcgccctgt cgatgatcaa actgcgtggc
     7141 tttggcggca aaactgagtt ctgggatcac aaccttgagt cgttcacctt gatggcgggg
     7201 ctggcggccg tcacctcgcg cattcagatt tacgccactg ctgccacctt aacgttacct
     7261 ccggcaatcg tcgcccgtat ggccgcaacc atcgactcca tctctggcgg gcgttttggc
     7321 gtcaacctcg tgactggctg gcaaaagccc gagtatgagc agatgggtat ctggcctggc
     7381 gatgactatt tctcccgtcg ttacgactat ctcaccgaat atgttcaggt gctgcgcgac
     7441 ctgtggggca cggggaaaag cgattttaaa ggcgattttt tcaccatgaa tgattgtcgc
     7501 gtcagtccgc aaccgagtgt ccccatgaaa gtgatctgcg ccgggcaaag cgacgctggc
     7561 atggcgttct ccgcccagta tgccgatttc aacttctgtt tcggcaaagg cgtaaatacg
     7621 cccacggctt tcgccccgac cgctgcgcgg atgaaacagg ccgcagagca aaccgggcgc
     7681 gacgttggtt cttatgtgtt gtttatggtg attgccgatg aaaccgacga tgccgctcgc
     7741 gccaaatggg aacactacaa agcgggcgcg gatgaagagg cgttaagctg gctaaccgaa
     7801 caaagtcaga aagatacccg ctccggtact gataccaacg tccgtcagat ggccgatccc
     7861 acttcggcgg taaacatcaa tatggggacg ttagtcggtt cttacgccag tgtcgcgcgc
     7921 atgttagatg aagtcgcaag cgtgcctggt gccgaaggcg tgctgttgac cttcgatgat
     7981 tttctgtcgg gaatcgaaac cttcggcgag cgcattcaac cactgatgca gtgccgcgcc
     8041 catctacctg cgctgactca ggaggtggca tgatgacaac cttaaccgct cgaccggaag
     8101 ccattacctt cgatccgcag caaagtgcgc tgatcgtggt ggatatgcaa aacgcttatg
     8161 ccacgccagg cggctactta gatctcgccg ggtttgatgt ctcaaccact cgcccggtca
     8221 ttgccaacat tcaaaccgcc gtgaccgcag cgcgagcggc agggatgctg atcatctggt
     8281 ttcaaaatgg ctgggatgaa cagtatgtcg aagctggcgg ccccggctca ccgaattttc
     8341 ataaatcgaa cgccctgaaa accatgcgta agcagccgca gctgcagggg aaattgctgg
     8401 cgaaaggctc ctgggattat caactggtgg atgaactggt gccgcagcct ggcgatattg
     8461 tgctgccgaa gccgcgctac agcggtttct tcaatacacc gctggacagc attttgcgca
     8521 gccgtggaat acgccatctg gttttcaccg gtatcgctac caacgtctgc gtcgaatcga
     8581 cgctacgcga tggctttttt ctggagtatt tcggcgtggt gctggaagac gcaacacacc
     8641 aggcggggcc ggaatttgca cagaaagccg cgttgttcaa tatcgaaacc ttttttggct
     8701 gggtcagcga cgtcgaaacg ttctgcgacg cgctttctcc cacatccttt gctcgtatcg
     8761 cttaaggagt ttaacgatgc caaaatccgt aattattccc gctggcagca gcgcaccgct
     8821 ggcccccttc gttcccggca cgctggctga tggcgtggtg tatgtctccg gtacgctggc
     8881 ttttgatcaa cataataacg tgctgtttgc cgatgaccca aaggcgcaaa cccgccacgt
     8941 tctggaaatt atccgcaagg tgatcgagac ggcgggtggc acgatggcgg atgtgacctt
     9001 caacagcatc tttattaccg actggaaaaa ttacgccgcg attaacgaaa tctacgccga
     9061 gttttttccg ggtgataaac cggcgcgatt ctgcattcag tgcggactgg taaaacctga
     9121 cgcgctggtg gaaatcgcca caattgcgca tatcgccaag tgaggccgcg atgaaacttt
     9181 cactctcacc tcccccttat gctgatacgc ccgtagtggt gttgatttcg ggtcttggcg
     9241 gtagcggcag ttactggtta ccgcaactgg cggtgctgga gcaggagtat caggtagtct
     9301 gttacgacca gcgcggcacc ggcaataatc ccgacacgct ggcagaagat tacagtatcg
     9361 cccagatggc agcggaactg catcaggcgc tggtagccgc agggattgag cattacgcgg
     9421 tggtcggcca tgcgctcggt gcgctggtgg gaatgcagct ggcgctggat tatcccgcgt
     9481 cggtaactgt gctgatcagc gttaacggct ggctacgaat aaacgcccat acgcgccgct
     9541 gttttcaggt tcgcgaacga ttactgtata gcggcggcgc gcaggcatgg gtggaagcgc
     9601 agccgttgtt cctctatccc gccgactgga tggcggcccg cgcacctcgc ctggaggcag
     9661 aagacgcgct ggcactggcg cattttcagg gcaaaaataa tttactgcgt cgacttaacg
     9721 ccctcaaacg cgctgacttt agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca
     9781 tctgcgccag tgatgatctg ctggtgccat cagcatgttc cagtgagctt catgccgccc
     9841 tgcccgatag ccagaaaatg gtgatgccct atggcggaca cgcctgcaac gtgaccgatc
     9901 ccgaaacgtt taatgctctg ttactcaacg ggcttgccag cctgttacat caccgtgaag
     9961 ccgccctgta aggaattgct atgaacgaag ccgttagccc aggtgcgctt agcaccttgt
    10021 tcaccgatgc ccgcactcac aacggctggc gggagacacc cgtcagcgat gagacgttac
    10081 gggagattta tgccctgatg aaatgggggc cgacatcagc taactgttct ccggcacgga
    10141 tcgtgtttat ccgcacggca gaaggaaaag aacgtctgcg cccggcactt tccagcggca
    10201 atctgcaaaa aaccctgacc gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt
    10261 atgaacggtt accgcaactg tttcctcacg gcgatgcccg cagttggttt acctccagcc
    10321 cacaacttgc cgaagaaaca gcgtttcgca acagttccat gcaggcggcc tatctgatcg
    10381 tcgcctgccg tgcgctggga ctggataccg gcccgatgtc gggttttgac cgtcaacacg
    10441 ttgacgacgc cttttttgcg ggcagcacgc tgaagagcaa tctgctgatt aatatcggct
    10501 atggcgatag cagcaaactt tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg
    10561 ggctgttgta aggagcaaac catgaacatt gtcgatcaac aaacctttcg cgatgcgatg
    10621 tcctgtatgg gcgcggcagt caatatcatc accacggatg gtccagccgg gcgcgccggg
    10681 ttcaccgcca gcgcggtctg cagcgtgacc gacacgccgc ctacattact ggtgtgcctg
    10741 aatcgtgggg cgtccgtctg gccggtattc aatgaaaatc gaacgctgtg tgtaaataca
    10801 ctgagcgccg ggcaggagcc gctttcaaat ctttttggcg gcaaaacgcc catggaacac
    10861 cgctttgccg ccgcccgctg gcagacgggt gtgaccggat gcccgcaact ggaagaggct
    10921 ctggtttcgt ttgactgccg tatcagccag gtggtgagcg tcggcaccca cgacattctg
    10981 ttttgcgcca tcgaagcgat tcatcgtcac gccacaccct acgggctggt gtggttcgat
    11041 cgcagttatc acgcgctgat gcgccctgct tgttaacctc ctaaaggaga cagctgatgg
    11101 caatgttcgg ttttcctcac tggcagttaa aatcgacctc tacagagagc ggcgtggtcg
    11161 cgccgtatga acgattgccg tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg
    11221 cgatgtttgg cgcgacggtg ttaatgccga ttctgatggg gctggatccc aatctttcca
    11281 ttttaatgtc ggggatcggc acgttactgt ttttctttat taccggcggg cgcgtcccca
    11341 gttatctcgg ttccagcgcg gcttttgtcg gcgtggtgat tgccgcgacg ggctttaacg
    11401 gtcagggcat taacccgaat atcagcattg cgctgggcgg gattatcgcc tgtgggctgg
    11461 tttataccgt cattggcctg gtggtgatga aaattggtac acgctggatt gaacgactaa
    11521 tgccgccggt ggtgacgggt gcggtggtga tggcgattgg cctgaactta gcgcctatcg
    11581 cagtgaaaag tgtatcggct tcggcctttg atagctggat ggcggtaatg acggtgctgt
    11641 gtattgggct ggtggcggtg tttacccgtg gcatgatcca gcggttattg attctcgtag
    11701 gtttaattgt cgcctgtctg ctgtatggcg tgatgaccaa ccttttaggg ctgggaaaag
    11761 ccgtcgattt tactctcgtc agccatgctg cctggttcgg tttgccacat ttttccactc
    11821 ctgcttttaa tagtcaggca atgatgctga ttgcgcccgt ggcggtgatt ctggtggcgg
    11881 aaaacttagg tcatctcaag gcggtcgccg ggatgaccgg acgcaatatg gacccgtata
    11941 tggggcgggc attcgtcggt gatgggctgg cgacgatgct ttctggttct gtcggtggca
    12001 gcggcgtaac cacctacgcc gagaatatcg gtgtaatggc cgtgacaaaa gtctactcga
    12061 cgctggtgtt tgtcgctgct gccgtcatcg ccatgctgtt gggcttttca ccgaaatttg
    12121 gcgcgctgat tcataccatt ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg
    12181 ggctgattgc cgtcgcaggc gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga
    12241 acggtaattt gattatggtc gcagtaacct tagtgctggg cgcgggtgat tttgctctca
    12301 cgctgggcgg ttttacgttg ggagggattg gtacagcaac ctttggcgcg attttactca
    12361 atgcgttgtt aagcagaaag ttggttgacg ttccaccgcc agaagttgtt catcaggagc
    12421 cataaacaga aaaccgccgg gattaccggc ggtgatgact ttttgacgag cctaagaaaa
    12481 aaatgcattc gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc
    12541 acctttttta cctgcttcag atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg
    12601 ctgtccacct tttttacctg cttctgatgc tctttcgcgg tcttctgcaa aattgccgga
    12661 accgcctcga tggtttgcca ttactgacct ccgtctgggt atagacatca tattgcatag
    12721 aattaacgcg gcatatggcg gctgccatat tttcgctacg tgggactaag cttagtgaac
    12781 tgacgcgggc agacccgcgt ttggtaatat cctgcaacag cttcttagcg agaatagata
    12841 tcatctcaaa taattatcca taagccgctc ttatgattaa aaaaacgcga tgagataaaa
    12901 atgtgtcatt ttgcgacaaa attacgtgct tgtgaaagtt gttataaatc aaataagtgg
    12961 ttgtgaaatt tgcactctga aaaggacgtc ttatctttaa ataagtggta gcgaatcgct
    13021 acggaataga gataacacga ggagtggtta gaaatggcta aagttctggt gctttattat
    13081 tccatgtacg gacatattga aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtc
    13141 gatggcgcag aagttgtcgt taagcgtgta ccggaaacca tgccgccgca attatttgaa
    13201 aaagcaggcg gtaaaacgca aactgcaccg gttgcaaccc cgcaagaact ggccgattac
    13261 gacgccatta tttttggtac acctacccgc tttggcaaca tgtccggtca aatgcgtacc
    13321 ttcctcgacc agacgggcgg cctgtgggct tccggcgcac tatacggaaa actggcgagc
    13381 gtctttagtt ccaccggtac tggcagcggt caggaacaaa ctattacttc aacctggacg
    13441 acccttgcgc atcacggcat ggtaattgtc cccattggct acgcagcgca ggaattattt
    13501 gacgtttcac aggttcgcgg cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac
    13561 ggctcacgcc agccaagcca ggaagaactg tctattgctc gttatcaagg ggaatatgtc
    13621 gcaggtctgg cagttaaact taacggctaa tcttcaacag gaggatacgc atgccaactc
    13681 aagaagcgaa agctcatcac gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa
    13741 tagccgaggc cattttcgaa gtcgccgggt acgacgaaaa aatggcggaa aagatttggg
    13801 aagaaggtag cgatgaagtg ttagtcaaag cctttgctaa gaccgataaa gattcgcttt
    13861 tttggggcga acagaccatc gaacgtaaaa acgtttaacc agcgactccc ccacttctcg
    13921 cgggggagtt ttctgttatt tcaccgcctc attcaacacg ctatcaaact tatccattgg
    13981 gcagaaaccg ttggcatcta tcgggcaacc gcttaattcc agcgtcacac gctgagcagg
    14041 tgcctgcagg gttaacgcat cggcattacg taactgttcc gcactctgat acacatattc
    14101 aattttcatc aaatcgcgat tggctttgct gtcatgccaa cgctggaaaa cgattttgcc
    14161 gccaatcggc gtgcgttcgt tctggtcatg caactgatac ggtttgaaat ccagcgccgt
    14221 taacagagag gcaatgttgg agtcgtgccc aaccaacact gtaattttcg gtgcgctggt
    14281 gcgatcggtg accagagctt tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc
    14341 cacttccggt gaggtaaaca ggctgtcctg gtagccgttt ttaagcttcg acaacacctt
    14401 ccactgctgg tcagatttga tttctcccca ggccacctga tccatcggaa aaccttcgta
    14461 atattgcaaa gtaaacgcat ctaccagcga gttgccgact ttcagcggcc cggaaacacc
    14521 tggttcttgt tgatacttgg cgctaaaggt atttttgcca tccaccagcg aacactgttg
    14581 tttctcttta caggcagggg aatctttata gttaacgatt ttttccagta gttggtagct
    14641 gtcggtaagc tggagtttgc tgagctcttt ctccattgcc gccaccgcct gttcactgaa
    14701 tgcggcggaa tcatcggtga tcaccgggtt aaaggttggg tccatagtgc ccattttttc
    14761 ctggtgatgc acaggaatat cacaccccgg gaatgcgccg gtaataaaga actgtgcggt
    14821 cgcaacggta cgttgcagac tattggcata ggcataaacg gtgtccggcg gcgggcattc
    14881 ccccgatttc accatcccct gctgtgccag ccattcacgc atgtaatggc ccatatacac
    14941 ttcgagcacg ccacctttgg tggtgagttg cccaccgggg acgtcccatt cgggccattt
    15001 attcggcgta gactgctcca gcacactgcc attgttcgcc agcggcgcac gtaagttatg
    15061 gcggctcatc atgagcactt gctgtagctg atagccttcc ggtacggttt gtgcctgagc
    15121 atttgaagcg agtaaaacta tccctgccac agttgcggcg attagcgttt tgttcattcc
    15181 tggcacctct tttgttatca atcactaaag agtgtgacag aaatatgaca tttttccggg
    15241 aacaggttcg caaaatggct tgttgaatgc ctgtttttct atatacatct ttaaaaacag
    15301 gcaaggtttg acgcaaacta tgcctgatta gctccctgcc tcgcgtacgc cgctgatgat
    15361 tacacctgtg gctaaacaga atatcagcgg cgttactaat acagaggcaa tagtaccaat
    15421 aataagagaa gtgaggctaa taacaatggt ttttagcaca tgcctttgtc agtaaattgc
    15481 ataacgagtt aaaaaatcaa tttgcgcatt cctttcctta ttgtttaact cgtaagcctg
    15541 agcaaaaagt tcaggaggga ggtaaacgtc ataatgattg ccagtgttta ttaaataccc
    15601 atactcaagg tcgtcatctg ttgaaaagga ttcattccct aaggagattg atgccacatt
    15661 accattaaca ataaataatg agcaaatgct atctgaaaga gcagtgagga aatttttcac
    15721 ctcattaacc ttctgctctg ggtcggtgtc taaaacaagg cgtgccttat tatcattaaa
    15781 tataaactcg ggtaaatgca gta
//
LOCUS       DAZKGS010000103        15428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKGS010000103 DAZKGS010000000
VERSION     DAZKGS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(172..978)
                     /gene="trpA"
                     /locus_tag="ACHOQY_003239"
     CDS             complement(172..978)
                     /gene="trpA"
                     /locus_tag="ACHOQY_003239"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9679288.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(978..2171)
                     /gene="trpB"
                     /locus_tag="ACHOQY_003240"
     CDS             complement(978..2171)
                     /gene="trpB"
                     /locus_tag="ACHOQY_003240"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9679289.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2183..3544)
                     /gene="trpCF"
                     /locus_tag="ACHOQY_003241"
     CDS             complement(2183..3544)
                     /gene="trpCF"
                     /locus_tag="ACHOQY_003241"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9679290.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3545..5140)
                     /gene="trpD"
                     /locus_tag="ACHOQY_003242"
     CDS             complement(3545..5140)
                     /gene="trpD"
                     /locus_tag="ACHOQY_003242"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9679291.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5140..6702)
                     /gene="trpE"
                     /locus_tag="ACHOQY_003243"
     CDS             complement(5140..6702)
                     /gene="trpE"
                     /locus_tag="ACHOQY_003243"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9679292.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6724..6817)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6794..6838)
                     /gene="trpL"
                     /locus_tag="ACHOQY_003244"
     CDS             complement(6794..6838)
                     /gene="trpL"
                     /locus_tag="ACHOQY_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9679293.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6976..7857
                     /gene="rnm"
                     /locus_tag="ACHOQY_003245"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6976..7857
                     /gene="rnm"
                     /locus_tag="ACHOQY_003245"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9679294.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7854..8474
                     /gene="yciO"
                     /locus_tag="ACHOQY_003246"
     CDS             7854..8474
                     /gene="yciO"
                     /locus_tag="ACHOQY_003246"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9679295.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            8575..9447
                     /gene="rluB"
                     /locus_tag="ACHOQY_003247"
     CDS             8575..9447
                     /gene="rluB"
                     /locus_tag="ACHOQY_003247"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9679296.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRCV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9487..10077)
                     /gene="cobO"
                     /locus_tag="ACHOQY_003248"
     CDS             complement(9487..10077)
                     /gene="cobO"
                     /locus_tag="ACHOQY_003248"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9679297.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(10074..10832)
                     /gene="yciK"
                     /locus_tag="ACHOQY_003249"
     CDS             complement(10074..10832)
                     /gene="yciK"
                     /locus_tag="ACHOQY_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9679298.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11052..12101
                     /gene="sohB"
                     /locus_tag="ACHOQY_003250"
     CDS             11052..12101
                     /gene="sohB"
                     /locus_tag="ACHOQY_003250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9679299.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12137..12388)
                     /gene="yciN"
                     /locus_tag="ACHOQY_003251"
     CDS             complement(12137..12388)
                     /gene="yciN"
                     /locus_tag="ACHOQY_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9679300.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12768..15365
                     /gene="topA"
                     /locus_tag="ACHOQY_003252"
     CDS             12768..15365
                     /gene="topA"
                     /locus_tag="ACHOQY_003252"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9679301.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         3751 a         3923 c         4136 g         3618 t
ORIGIN      
        1 tgcgggatta atttgtttga ttatattact gttgggcgga aaaatgacgt aagttgacgc
       61 tcgaccgggg taagcgaaac ggtaaaaaga taaatattaa atgaatttag gatttttccg
      121 gcttcattaa agaaagttaa aatgccgcca gcggaactgg cggctgtggg attaactgcg
      181 cgtcgccgct ttcatcggtt gtacaaaaac tttcagtgcc gccagcattt tctctggctc
      241 attaatatgt tgctcgatga ttttaacaat ggccgaacca gaaatcgcgc ccgcagctcc
      301 tgcatcaatc gctgctttta cctgatccgg ggcggaaata ccaaatccct gcaatggagg
      361 tgcagcgttg tactctttca gcttcgcaac cagatgattg aggggtaacg cggcgcggtt
      421 ttctgcgccg gtcacgcctg ctcgtgacag caaataggtg taaccacgac cgtaagaggc
      481 tatctggcgc agcaggtcgt catcggcatt tggcgggcag atgaagatag gtgcgacatt
      541 atgacgcaac gcggcctggc ggaagggcgc ggactcttca actggcacat cggcaaccag
      601 caccgaatcg acgccgactt tttcgcactg ggcataaaac tcatcaatgc ctttgttaaa
      661 caccagattg gcatacatca acaggccaat gggaatggtc gggtgtttct ggcgaatcag
      721 tgccagcatt tcaaaacatt gtgccggagt cacacctgcc gcaaaggcgc gcagagtggc
      781 gttttgaatc gtcgggccat ccgccagtgg gtcggagaag gggataccta actccagcgc
      841 gtcagcaccg gcttcaatta gcgtatcgat aattttcaat gactgctcaa tgcccggatc
      901 accgagcgtg acgaaaggaa cgaatgcgcc ttctttgcgc tccttcaact gggcaaacag
      961 agattcgtag cgttccatca gatttcccct cgtgctttca aaatatcgtg aacggtgaag
     1021 atgtctttat cgccgcgacc ggaaaggtta accaccagta gctgctcttt atccgggttt
     1081 tcgcgcatca ttttcaacgc atgggccagg gcgtgggagg attccagcgc cgggatgatc
     1141 ccttcgtgca ggcacagcgt tttgaaggct tcaagggctt catcatcggt aatagacacg
     1201 taatcagcgc gtccagtgct gttaagatac gcgtgttgtg ggccgacaga cgggaaatcc
     1261 agtccggcgg agatggagta agattcttca atctgcccgt cttcggtttg catcatcggc
     1321 gctttcatac cgaaatagat acccacgcga ccatgtttta gcggtgcgcc gtgctcgcca
     1381 gtttcgatac cgtgaccacc tggctccaca ccaatcaggc cgacgttggt ttcattgatg
     1441 aaatcagcaa acatgccgat ggcattcgaa ccgccgccaa cacaggcgat aacggcatcc
     1501 ggcaggcgac cttctctttc cagaatctgc gctttggttt cttcgccaat catccgctga
     1561 aactcacgca caatggtcgg ataaggatgc gggccagctg cggtgcccag catatagtgc
     1621 gcggtttcgt aactaccgga ccagtcgcgc agcgcctcgt tacaggcatc tttcagcgtc
     1681 gcggaaccgc tatgcaccgg gatcacttcc gcacccatta agcgcatacg aaaaacgtta
     1741 ggcgactggc gttcaacgtc tttggcaccc atataaatac ggcatttcag gccgagcagg
     1801 gcgctggcaa gggccgacgc cacgccatgc tgaccggcac cggtttcggc gatgatttcg
     1861 gttttaccca tccgcttcgc cagcaacgcc tgccccagca cctggttagt tttatgcgcg
     1921 ccgccgtgca gcaaatcttc acgcttgaga tacagcgtag tgttcgtccc ggctgtaatg
     1981 ttctggcatt tggtcagcgc ggttggacgc ccggcatagt ttttcagcag gtcgttgaac
     2041 tgagcctgaa attcaggatc tttttgcgca ctgacaaaag cttcttccag ctggcgcaga
     2101 gcaggcatca ggatttgtgg cacgtacatg ccgccaaact caccaaaata ggggttaagt
     2161 aatgttgtca ttgttccttt ccttaatatg cgcgcagcgt ctggaaaacc gaggccaaaa
     2221 gacgtgcgtc tttgatgccc ggttgcgact ctacagcaga attaaaatca agtccggcgc
     2281 agccggtttg tgccgcttcc acgcagttat ctgcgcctaa gccccccgcc agcagaacgt
     2341 tgccaagcga ttgaccattt aatagtgacc agtcaaaacg ttgcccgctt ccaccctggc
     2401 cgttgtctaa aacatattta tcaacgtgct gaaactcgcg ggcgggcagg gtttcaccga
     2461 cgcttaatgc tttccagatg gcaacatgtg ctggcagagc ttcacgcagc gtatcgatat
     2521 acagctgttc ttcattacca tgcagttgca ctgccgccag cgataacacc ttagctttgt
     2581 ccaccacatc ggcaatatcg tgattgcgga acacgccaac atactgcaac ggtgccgcag
     2641 ccatcacttc ctgcgcctgt tcaacgttga cgcaacgcgg tgatgtcgca acaaaaatca
     2701 acccaccgta aatcgcgccc gcgtcataag ctgctttagc atcttgccca cgcgtcaggc
     2761 cacatacttt attctcaccc agcaacaccc ggcgcacggc ggcgtgcaaa tcgtcatggg
     2821 ccatcaacgc cgaaccaatc agaaaaccgt tagcgaagtg gcttaactcg cgcacctgag
     2881 cgtaagtatt gatgccggat tcgctgatta ccgtcacgtt gtgccccagt ttcggcgcaa
     2941 gctcgcgggt acggttgaga tcaatcgaca aatcacgcag atcgcggttg ttgatgccaa
     3001 cgacctttgc ccccaatgca atggcacgct ccagttcctc ttcattactg acttcggtca
     3061 gcacacccat ctccagactg tgggcgacgg cggcaagctg gcgatattgt tcgtcatcca
     3121 gtactgaaag cattaataag caggcatcag cctggtaata gcgcgccaga tagatctggt
     3181 aaggatcgat aatgaagtct ttacataaaa tcggctgcgg ggcgatttgg ctgacgatgg
     3241 ggaggaaatc aaagctcccc tgaaaatatt tctcatcagt cagcaccgaa attgccgaag
     3301 cgtaatgttt ataaatggcg gcaatgcgtg ctggatcgaa atcatcacgg atcacgcctt
     3361 ttgacggcga cgatttcttg cactccagaa taaacgccgt gcgcgcaccc tgtagcgcat
     3421 cataaaaatg tcgcgtgctc ggctgaacct cattctgaaa actggccagc ggttgctgct
     3481 gtttgcgggt ttctacccaa atcgccttgt ctgcgacgat tttcgctaaa acggtttgca
     3541 tcatttaccc tcgtgccgcc agtgcggtaa ctctgtcgta agcggaacca ctgcgcagta
     3601 cctcaagaac ggtttgcgca ttggcttgca gatcttcatg gccatgcagg cgcattaaca
     3661 tggcgacgtt cgcagcgacg gctgcttcat gggcggcgtc gcctttacct tgtaacaagc
     3721 gtgttaaaat gtcacggttt tcttccggtg ttccgcctgc cagttgctcc tggtggtagg
     3781 gagtcaggcc aaaatcttca gcggtcaatt gatagctctt aatttcgccg tcatgcagct
     3841 cggcaacgat tgtcggcgcg tgtaatgaaa cttcatccat cccgccgctg tgcaccaccg
     3901 ccgcgcgttg ataccccagc acgcgcaagg tttcggcaat cggcagcacc agttccggac
     3961 tataaacacc aattaacgcc agcggcggat gcgccgggtt aatcaatggc cccagcacat
     4021 tgaacagggt gcgggttttc agttgctggc gaaccggcat cgcgtggcgg aatccggtgt
     4081 gatacttcgg cgcaaagagg aaacatacac ctaactcatc cagcgcctgg cgcgatttat
     4141 cggcgttcat atcaagatta ataccgaacg ccgccagcag atcggacgaa ccagatttac
     4201 tggagacgct acggttgccg tgtttcgcca ctttcagccc acaggccgcg gcgacaaacg
     4261 cactggcggt agaaatattg atactgttgc tgccgtcacc gccagtaccg acgatatcag
     4321 caaacagata atccgggcgc gggaacggcg cggcgttttc cagtagcgcg gttgctgctc
     4381 cggcgatctc gttcgggtgc tcaccgcgaa ttttcatgct caccagcgcc gccgccagtt
     4441 gttccggctt cagctcgcca cgcaccaccg ctgaaaacag ctggtggctt tcttgttggc
     4501 taagcgtctg cgcctgatac agtttttcca gaatcggttg cagcgtgttg gctggctcta
     4561 gtttctgctg cgcccaggcc agcgtttgtt ccagcaggcg agcgccctgg gtggtgagaa
     4621 tggattccgg atggaactgg aatccacaaa cgcgatccgc atcgtgacgt actgccatca
     4681 ccatgccatt aaaatgggcg ttgatggtta aaccggccgg aatgttactg ccaaccagcg
     4741 agtgataacg cgccaccggc agcgggtttg ttaatccggc aaacatcgcc tgaccgtcat
     4801 gttcaatgct cgacgcttta ccgtgaagaa tttcgcccgc ctgaccgaca tagcccccgt
     4861 aagcttcgac aattgcctga tgtccgaggc aaatgccaat aatgggcagc ttgccacgca
     4921 agcgggtgag gagttccggc atacaaccgg cttcgctcgg cacaccgggg ccaggagaaa
     4981 gcatcagtac cggattgctc atcgtcgcca ggcgttcaat taaggtctgc gccggaatat
     5041 ggttgcggta aatcaccaca ttatgcccat tgctgcgcaa ctgatctgcc aggttgtacg
     5101 taaaagagtc gatattatcg agcagcagaa tgtcagccat cagaaagtct cctgtgcatg
     5161 atgcgcggtg gcaatagcgc gcagtacagc gcgggcttta ttacgggttt cgtcggcttc
     5221 cgactgcgga acagaatcaa ggactacgcc agcaccggct tgcacggtgg cgataccgtt
     5281 ttccaccagc gccgagcgga tcacaatgca ggtgtcgaga tcgccatgcg cggtgaaata
     5341 acctaccgcg ccgccgtagc tgccgcggcg acgaccttcc gcctcggcaa ttaactgcat
     5401 agcgcgtact ttcggcgcac cacttaacgt tcccatattc atacaggcgc ggtaagcgtg
     5461 cagggcgtcg agatcgtggc gcagctcacc gaccacgcgg gagaccaagt gcatcacgta
     5521 agagtaacgg tcaactttgg tgaggtcggc gacgtagcgg ctgccggggg tgcaaatgcg
     5581 tgccagatca ttacgggcga gatccaccag catcagatgt tcagaaagct ctttatgatc
     5641 ggtacgcatt tccagttcga tgcggctgtc gaggtctctg tccagtgaac catcggcacg
     5701 acgaccgcgc ggtcgtgttc cggcaatcgg gtagatctca atctggcggc tggtggcgtc
     5761 atacttgagc gaactttccg gcgacgcgcc aaacagggtg aaatcattat cctgcataaa
     5821 aaacatgtac gggctgggat tactcttttt cagcacgtaa taggccgcca gcggtgacgg
     5881 gcagggcaga gagaaacggc gagatggcac cacctggaaa atttctccgg cgcgaatcgc
     5941 tttttgcaac aaacgcacta cgccaccgaa ctcttcatcg ctctggttac attcacaacg
     6001 catatgcggc acggaaacca ccggcagcgg tggcgcggtt tcggtcagtt gctggcgaag
     6061 atcgttcagg cgagcagtga gacgttgttt ttcttcttca ttcggagcaa acaggctggc
     6121 ctgaatgcgg gtgctttttt tctgatggtc aatcaccatc agcgtttcag cgagataaaa
     6181 acagaaatca ggacagttat tttccgctga caattgcggt aaatcttcaa accccgccac
     6241 aaggtcataa gagaacaggc cgccgaagaa cattgcttct cgttcttcct tcggtacatt
     6301 cagcatattc tgcaataagc ggaaagcgtc aaaaaccgaa agggagcata agcgagcgtc
     6361 ttcatccagc agtggactga caggggggaa gcgcagcaca cggcagtttg gtaattgttc
     6421 attttccata cccgcaggca gggcgttatc cagtagtgtc agcagggctt cgccgttgcc
     6481 ggaaagtgcc tggattgtga cagtgtcacc taaagctgta atgcgcagcg cactgtctac
     6541 cagcagcagg ctttttaaat catctttgct gtcgatatct gcggattcca gcagcagcgt
     6601 tgccggacga tccccacaca actggtgaaa aagcgcggtg ggattgtcgc gataagcgcc
     6661 ttcgcaggtt agcagttcga gagtcggttt ttgtgtttgc attgttattc tctaattttg
     6721 ttcaaaaaaa agcccgctca ttaggcgggc tgggtatctg attgctttac gcatggtgaa
     6781 tacactgccc gtttcaggaa gtgcgccacc aacctttcag tacgaaaatt gctttcattg
     6841 tcgataccct ttttacgtga acttgcgtac tagttaacta gttcgatgat taattgtcaa
     6901 tagctcattt cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca
     6961 gaacgggagt gcgccttgag cgacacgaat tatgcagtga tttacgacct gcacagccat
     7021 accacagctt ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgagatg
     7081 cgcgtcggca ccctggcgat caccgaccat gacaccacag ctgcaatcgc gccagcaaga
     7141 gaggaaattt cacgttcggg actggcgttg aatcttattc ccggcgtgga aatttccacg
     7201 gtctgggaaa atcatgaaat tcatattgtc gggctgaata ttgatattac tcatccgcta
     7261 atgtgtgagt tcctcgcgca gcagacagaa cggcgcaatc agcgggcgca gctgattgcc
     7321 gaaagacttg aaaaagcgca aatacctggg gcgctggaag gcgcacaacg actggcgcag
     7381 ggcggcgcag tgacgcgcgg tcattttgcg cgtttccttg ttgagtgcgg caaagccagt
     7441 tcaatggcgg atgtctttaa aaagtatctg gcgcgcggga aaaccggata cgttccgcca
     7501 cagtggtgta caatagaaca agctattgat gtcattcatc attctggcgg taaggcggtg
     7561 ctagctcatc ctgggcggta caatcttagt gctaaatggc tgaaaagatt ggttgcgcat
     7621 tttgccgaac accacggtga cgcgatggaa gtcgcgcagt gccagcaatc gcccaatgaa
     7681 cgtacccagc tggcggccct tgcgcgtcag catcatttat gggcatcaca aggatctgat
     7741 tttcatcagc catgcccgtg gatcgaactg ggtcgtaaac tctggctgcc cgcaggcgtt
     7801 gaaggcgtct ggcagctatg ggaacagccg cagaacacca cagagaggga attatgagcc
     7861 agttttttta tattcatcct gataacccac agcaacgtct gatcaaccag gcggtggaga
     7921 tcgtgcgtaa aggcggggtg attgtttatc caactgattc cggctatgcg ctcggctgta
     7981 aaattgaaga taaaaacgcg atggagcgta tttgtcgtat tcgccagctg ccggacggtc
     8041 acaactttac cctgatgtgt cgcgatcttt ctgaactctc gacctattca tttgttgata
     8101 acgttgcgtt tcgtttaatg aaaaacaaca cgccgggcaa ctataccttc atcttgaagg
     8161 ggacgaagga agtgccacgc cgtctgttgc aggaaaaacg caaaaccatc ggtatgcgtg
     8221 tgccttctaa cccgattgcg caggcgttgc ttgaagcact gggcgaaccg atgctttcca
     8281 cttcgctaat gctgccaggc agcgaattta ccgaatccga tcccgaagaa attaaagacc
     8341 gtctggaaaa acaggtagat ttgattattc acggcggcta tctcgggcag aaaccgacaa
     8401 cggttattga tcttaccgat gataagccgg tcgtggtgcg tgaaggcgta ggtgatgtga
     8461 agcctttctt ataagtgcgt aactctgtat actacgcggc cttaaaatgg cgctatacgg
     8521 cgccattgat tctcctgttc gacgcctgtg aaggcgacac taaaaggaag ctctatgagc
     8581 gaaaaattac agaaagtgct ggcccgcgct ggccacggct cccgccgtga aatcgaatct
     8641 attattgaag ccggtcgtgt gagtgttgat ggcaaaattg ctaaactcgg cgatcgcgtt
     8701 gaagttaccc ctgggctgaa aattcgtatc gatggtcatc tgatttcggt acgtgagtcc
     8761 gctgaacaaa tttgtcgcgt tctggcctat tacaaaccgg aaggtgagtt gtgtacccgt
     8821 aacgatccgg aagggcgtcc gacggtgttt gaccgtctgc caaagctgcg tggcgcacgc
     8881 tggattgccg taggtcgtct ggacgttaac acctgtggtc tgctgctgtt caccaccgat
     8941 ggtgaactgg caaaccgttt aatgcatcca agccgtgaag ttgaacgtga atacgccgtg
     9001 cgtgtatttg gtcaggttga cgacgcgaaa ctgcgtgatt tgagtcgttg cgtgcaactg
     9061 gaagatggtc cggcagcttt taaaaccatc aagttcagcg gcggcgaagg gatcaaccag
     9121 tggtacaacg tgactctgac cgaaggccgt aaccgtgaag ttcgtcgtct gtgggaagcg
     9181 gttggtgtgc aggtgagccg cctgatccgt gttcgttacg gtgatatccc actgccaaaa
     9241 ggtctgccac gcggtggctg gacggagctg gacctcgccc agactaacta tctgcgcgaa
     9301 ctggtggagc taccgccaga aaccagctct aaagtcgctg tagaaaaaga ccgtcgtcgc
     9361 atgaaggcga atcagattcg tcgtgcggtg aaacgtcaca gtcaggtgag cggcggtcgt
     9421 cgttctggcg gacgtaataa cggttaatca aagtatgccg gacgtcatat ccggcatttt
     9481 tacagattag taatcaatcc ctatctgcgc ttttacaccg gcatcaaacg catgtttgac
     9541 ggggcgtaat tcacttaccg tatctgccag ttcaagaata tcccgatgac aaccacgacc
     9601 cgtgataatc accgtctgtt gatgtggacg ttcatttaac gcctgcacca cttcttccag
     9661 tggcaaatag tcatacgcca ccatatacgt cagttcatca agcaaaacca tatccagtga
     9721 ggaatcagca agcatccgct ttgcatgttg ccagacttcg cggcaggcgg cggtatcaga
     9781 ctcgcggttt tgtgtatccc aggtaaagcc cgttgccatc acctgaaact caacgccatg
     9841 tggctccagc agattgcgtt cgccattagg ccaggtgcct ttaataaact gcacgacacc
     9901 tacttttttt ccgtgaccaa ctgcgcgtgt tgccgtacca aatgccgcgg tggttttgcc
     9961 ttttccattg ccggtaaaga cgatgataat accgcgttca tcctgggcct gggccacacg
    10021 agcatctact ttttctttca ctcgctgctg acgctgttgg tagcgttcat cactcattgg
    10081 gaaattcctg gtttacggcc cggttgggcg tcaaaggtca tgccggtttt acggcggctg
    10141 tcatcgccca tcagccagag gtagagcggc atgatatcag cgggtgtttt aagtttctgt
    10201 ggatcttcgg tcgggaaggc gctggcacgc attgcggtgc gcgtaccgcc tgggttaatg
    10261 cagttgacgc gcaggcgctg ctgatattca tcggccagta cctgcatcat cccttcggtg
    10321 gcaaatttcg acgctgcata tgcaccccag ttagctcgtc cctgacgtcc aacgcttgat
    10381 gaagtaaaga ccagtgaacc ggcgtccgat ttgagtaata aaggaagcag cgcctgggtg
    10441 agcaaaaagg tggcattaac gttgacctgc atgacgtcct gccagacctg cggattttgt
    10501 tcgctcattg ggcaaacatc gccgagcaat ccggcattat gcaaaacacc atccagacgc
    10561 ggataattaa cggtaatgcg ctgtgccagt tgttggcaat cttcggacgt gcaggtcagc
    10621 aaatcgagaa taaaccactg tggctgacgt ccagtttctt cgtttatgtg gctggctacc
    10681 tgacgtaatt tttcttcatt acggcccaac agaatcactg tcgcaccata gcgtgcatac
    10741 gtcatcgcgg cttcacggcc aatcccatcg ctggctcccg tcaccaggat aatgcgatca
    10801 ttgagtaaat cttgttttgg ctggtaatgc atggctactc ctcaacgacg ttgtctgtcg
    10861 cgctgccagt gtactttatg actttggggc tttatgcctg aaacagaagg gcatttcaat
    10921 cagcctgggg cgataacacc gcaaaatgga tactttgtca tactttcgct gcaataacat
    10981 ctctgcgaga cggcttaaca tgcctgttgt aaactgtgag ccaaagcgtt gtttaaccaa
    11041 ggtggggact cgtggaattg ttgtctgaat atggtttgtt tttggcgaaa atcgttaccg
    11101 ttgtgctagc gattgcggcg attgccgcca ttattgtcaa tgttgctcaa cgtaataaac
    11161 gccagcgtgg cgagttacgg gtcaacaatc tcagcgaaca gtataaggag atgaaagaag
    11221 aactggccgc ggcgctgatg gactcacatc agcaaaaaca gtggcacaaa gcgcagaaga
    11281 aaaagcacaa gcaagaagcg aaagcagcaa aagcgaaagc caaactgggc gaggtggcaa
    11341 ctgacagtaa acctcgcgtc tgggtgctgg attttaaagg cagcatggac gcccatgaag
    11401 tgaactcgct acgtgaagag ataacggcgg tactcgcagc attcaaaccg caggatcagg
    11461 ttgtgctccg tctggaaagc cctggtggca tggtgcatgg ttacgggttg gcggcttcgc
    11521 agctgcagcg tctgcgtgat aaaaacattc ctttaactgt tacggtagac aaagtcgctg
    11581 ccagcggcgg ttacatgatg gcctgtgtgg cggacaaaat tgtttccgca ccgtttgcta
    11641 ttgtgggttc cattggggtg gtggcgcaaa tgcccaactt taaccgcttc ctgaaaagca
    11701 aagatattga tatcgaactg cacactgccg ggcagtataa gcgtactctg accttgctgg
    11761 gtgaaaatac cgaagaaggg cgggagaaat tccgcgaaga gttgaacgaa acgcatcagt
    11821 tgtttaaaga ttttgtgaag cgtatgcgtc cgtctctgga tattgaacag gtggcaacgg
    11881 gtgaacactg gtacggacaa caggcggtag agaaaggcct ggttgatgaa atcaacacca
    11941 gtgatgaagt tattcttagc ctgatggaag gccgtgaagt ggttaatgta cgctatatgc
    12001 agcgtaaacg actcattgac cgattcaccg gcagcgcggc agagagcgcc gatcgattgt
    12061 tgctacgctg gtggcagcgg ggtcaaaagc cattgatgta aaagacaaac gcgaggctaa
    12121 gacctcgcgt tttgctttaa tcaaccagat gatatttttc tgaaagcaca tgggccaggt
    12181 gtttgaacat attaaacacc gcggtgcttt tggctgttgg caatccttgt tcatctaaaa
    12241 agtagtcgcc ggtaaatacc agcacgccat tacgctgcgt gacgccggtg gcttcaatcc
    12301 ctgcgagcgt atcctcatgc tcacgaatga ttttgttggc ctctttcaac agcgtttcgc
    12361 ggtcgatggg ttgtgtctct ttgttcatta tttactcctt aaacaaggac attagtctac
    12421 gccaggcatg gcttgcagac aaatatacca cgctggtggc aagagcgcct tactggtaac
    12481 tttggatttt gcatgctaat aaagttgcgt atcggatttt atcaggtaca gtgtgacgct
    12541 ttcgtcaatc tggcaataga tttgcttgac attcgaccaa aattccgtcg tgctatagcg
    12601 cctgtaggcc aagacctgtt aactcagtca cctgaatttt cgtgaacaga gtcacgacaa
    12661 ggggttgata tccgcagaga acgagtccat atcggtaact cgttgccagt ggaaggttta
    12721 tcaacgtgcg acgcattcct ggaagaatca aattaggtaa ggtgaatatg ggtaaagctc
    12781 ttgtcatcgt tgagtccccg gcaaaagcca aaacgatcaa caagtatctg ggtagtgact
    12841 acgtggtgaa atccagcgtc ggtcacatcc gcgatttgcc gaccagtggc tcagctgcca
    12901 aaaagagtgc cgactctacc tccaccaaga cggctaaaaa gcctaaaaag gatgaacgtg
    12961 gcgctctcgt caaccgtatg ggggttgacc cgtggcacaa ttgggaggcg cactatgaag
    13021 tgttgcctgg taaagagaag gtcgtctctg aactgaaaca actggctgaa aaagccgacc
    13081 acatctatct cgcaaccgac cttgaccgcg aaggggaagc cattgcatgg cacctgcggg
    13141 aagtgattgg gggtgatgat gcgcgctata gccgagtggt gtttaacgaa attactaaaa
    13201 acgcgatccg ccaggcattt aataaaccgg gtgagctgaa tattgatcgt gttaatgccc
    13261 agcaggcgcg tcgctttatg gaccgcgtgg tggggtatat ggtttcgccg ctgctatgga
    13321 aaaagatcgc tcgtggtctg tctgccggtc gtgtgcagtc ggtggcggtt cgcctggtgg
    13381 tcgagcgtga gcgtgaaatt aaagcgttcg tgccggaaga gttctgggaa gtcgatgcca
    13441 gcacgaccac gccatctggt gaagcgttgg cgttacaggt gactcatcag aacgacaaac
    13501 cgttccgtcc ggtcaacaaa gaacaaactc aggctgcggt aagtctgctg gaaaaagcgc
    13561 gctacagcgt gctggaacgt gaagacaaac cgacaaccag taaacctggc gctcctttta
    13621 ttacctctac gctgcaacaa gctgccagca cccgtcttgg atttggcgtg aaaaaaacca
    13681 tgatgatggc gcagcgtttg tatgaagcag gctatatcac ttacatgcgt accgactcca
    13741 ctaacctgag tcaggacgcg gtaaatatgg ttcgcggtta tatcagcgat aattttggta
    13801 agaaatatct gccggaaagt ccgaatcagt acgccagcaa agaaaactca caggaagcgc
    13861 acgaagcgat tcgtccttct gacgtcaatg tgatggcgga atcgctgaag gatatggaag
    13921 cagatgcgca gaaactgtac cagttaatct ggcgtcagtt cgtagcctgc cagatgaccc
    13981 cagcgaaata tgactccacg acgctgaccg ttggtgcggg cgatttccgc ctgaaagcac
    14041 gcggtcgtat tttgcgtttt gatggctgga caaaagtgat gcctgcgttg cgtaaaggcg
    14101 atgaagatcg catcttacca gcagttaata aaggcgatgc tctgacgctc gttgaactta
    14161 caccagccca gcactttacc aagccgccag cccgtttcag tgaagcatcg ctggttaaag
    14221 agctggaaaa acgtggtatc ggtcgtccgt ctacttatgc gtcgatcatt tcgaccattc
    14281 aggatcgtgg ctacgtgcga gtagaaaatc gtcgtttcta tgcggaaaaa atgggcgaaa
    14341 tcgtcaccga tcgccttgaa gaaaatttcc gcgagttaat gaactacgac tttaccgcgc
    14401 agatggaaaa cagcctcgac caggtggcaa atcacgaagc agagtggaaa gctgtactgg
    14461 atcacttctt ctcggatttc acccagcagt tagataaagc tgaaaaagat ccggaagagg
    14521 gtggtatgcg cccgaaccag atggttctga ccagcattga ctgcccgact tgtggtcgca
    14581 aaatggggat tcgcacagcg agcaccgggg tattccttgg ctgttctggc tatgcgctgc
    14641 cgccgaaaga gcgttgcaaa accaccatta acctggtgcc ggaaaacgaa gtgctgaacg
    14701 tgctggaagg cgaagatgct gaaaccaacg cgctgcgcgc aaaacgtcgt tgcccgaaat
    14761 gcggcacggc gatggacagc tatctcatcg atccgaaacg taagttgcat gtctgtggta
    14821 ataacccaac ctgcgacggt tacgagatcg aagagggcga attccgcatt aaaggttatg
    14881 acggcccgat cgttgagtgt gaaaaatgtg gctctgaaat gcacctgaaa atggggcgtt
    14941 tcggtaaata catggcctgc accaacgaag agtgtaaaaa cacacgtaag attttacgta
    15001 acggcgaagt ggcaccaccg aaagaagatc cggtgccatt acctgagctg ccgtgcgaaa
    15061 aatcagatgc ttatttcgtg ctgcgtgacg gtgctgccgg tgtgttcctg gctgccaaca
    15121 ctttcccgaa atcgcgtgaa acgcgtgcgc cactggtgga agagctttat cgcttccgcg
    15181 accgtctgcc ggaaaaactg cgttatctgg ccgatgcgcc acagcaggat ccggaaggta
    15241 ataagaccat ggttcgcttt agccgtaaaa ccaaacagca atatgtctct tcggaaaaag
    15301 acggaaaggc gactggctgg tcagcatttt atgttgatgg caaatgggtt gaaggaaaaa
    15361 aataaccttt aattctgtca ggtttttata aacaaagggt cgcgaaagcg gccctttttt
    15421 attgcata
//
LOCUS       DAZKGS010000104        15392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKGS010000104 DAZKGS010000000
VERSION     DAZKGS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..329
                     /locus_tag="ACHOQY_003253"
     CDS             81..329
                     /locus_tag="ACHOQY_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9679302.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHOQY_003254"
     CDS             307..1437
                     /locus_tag="ACHOQY_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9679303.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOQY_003255"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHOQY_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9679304.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHOQY_003256"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHOQY_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHOQY_003257"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHOQY_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9679305.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHOQY_003258"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHOQY_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9679306.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHOQY_003259"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHOQY_003259"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9679307.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHOQY_003260"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHOQY_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9679308.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHOQY_003261"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHOQY_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9679309.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..7124
                     /gene="kch"
                     /locus_tag="ACHOQY_003262"
     CDS             5871..7124
                     /gene="kch"
                     /locus_tag="ACHOQY_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9679310.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHOQY_003263"
     CDS             complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHOQY_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9679311.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7495..8955
                     /gene="cls"
                     /locus_tag="ACHOQY_003264"
     CDS             7495..8955
                     /gene="cls"
                     /locus_tag="ACHOQY_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9679312.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8990..9319
                     /gene="yciU"
                     /locus_tag="ACHOQY_003265"
     CDS             8990..9319
                     /gene="yciU"
                     /locus_tag="ACHOQY_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9679313.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHOQY_003266"
     CDS             complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHOQY_003266"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9679314.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHOQY_003267"
     CDS             complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHOQY_003267"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9679315.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHOQY_003268"
     CDS             complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHOQY_003268"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9679316.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHOQY_003269"
     CDS             complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHOQY_003269"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9679317.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHOQY_003270"
     CDS             complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHOQY_003270"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9679318.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3933 a         3751 c         3640 g         4068 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
     6001 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
     6061 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
     6121 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
     6181 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
     6241 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
     6301 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
     6361 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
     6421 caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt
     6481 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
     6541 ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag
     6601 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     6661 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     6721 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     6781 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     6841 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     6901 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     6961 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     7021 atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg
     7081 aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag
     7141 agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac
     7201 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     7261 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     7321 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     7381 gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     7441 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     7501 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     7561 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     7621 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     7681 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     7741 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     7801 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa
     7861 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     7921 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     7981 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     8041 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     8101 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     8161 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     8221 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     8281 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     8341 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     8401 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     8461 caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc
     8521 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     8581 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     8641 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     8701 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     8761 gatatgcgga gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt
     8821 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     8881 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     8941 agtccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     9001 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     9061 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     9121 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     9181 tttgaaccca gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     9241 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     9301 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     9361 ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     9421 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     9481 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     9541 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     9601 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga
     9661 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa
     9721 attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga
     9781 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     9841 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     9901 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     9961 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
    10021 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
    10081 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
    10141 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
    10201 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
    10261 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
    10321 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
    10381 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
    10441 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
    10501 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
    10561 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
    10621 catcgcgcgc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
    10681 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc
    10741 gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag
    10801 tgggttcatc cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc
    10861 gctgacgcat gccgccagaa aattcgtgcg ggtacatttt catgcgttta cgcgcttccg
    10921 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
    10981 tcttatgcag catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg
    11041 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat
    11101 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt
    11161 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
    11221 ctacaatacc cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga
    11281 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
    11341 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
    11401 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
    11461 cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt
    11521 cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag
    11581 gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac
    11641 caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc
    11701 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
    11761 aatacgagcc atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt
    11821 ttgaccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
    11881 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
    11941 ataaagtgtc ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat
    12001 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
    12061 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
    12121 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
    12181 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
    12241 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
    12301 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
    12361 tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact
    12421 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca
    12481 taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa
    12541 gagagcacgg gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt
    12601 aaccctttcg cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca
    12661 cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc
    12721 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
    12781 aatatcatga ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc
    12841 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
    12901 ctaaccccca gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga
    12961 aaactggatg caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa
    13021 tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga
    13081 tgatatttct cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa
    13141 gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc
    13201 ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc ttctggcctg
    13261 tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta
    13321 ttgccattag tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt
    13381 gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg
    13441 aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg
    13501 cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata
    13561 atgcgcggta ttcatcgagc tgttcgaaag catggtgttc aggaaggaag ttggttcgtt
    13621 gtagtcagca caccagcctg cacgggccac atcaaaagta ccctggtgac gggtgtcgag
    13681 gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt tcttccacaa
    13741 tgaagaggca gcaatcgcca gctttttatg cagatcggag gtgttataca acaggttgat
    13801 ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt
    13861 acgtttttcc tggctccagc caaaccattc cggctgagtc aattttgcgc catcagtata
    13921 cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat
    13981 atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt
    14041 ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg
    14101 gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttataag tcatgtcgat
    14161 ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac
    14221 ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg
    14281 ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg
    14341 ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag ttgatgggtg
    14401 aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc
    14461 atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat
    14521 accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac
    14581 agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt
    14641 cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc
    14701 gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg
    14761 gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga
    14821 accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc
    14881 tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct
    14941 cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag
    15001 catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct gtcaggttac
    15061 tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct
    15121 tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt
    15181 aattgttaat tattctcatt accaataaat aatcgtctgg cggttgcccg gaaagtacca
    15241 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga
    15301 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa
    15361 tagagattgg ctaagcatgt ctatcttttc tg
//
LOCUS       DAZKGS010000105        15305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKGS010000105 DAZKGS010000000
VERSION     DAZKGS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(272..1096)
                     /gene="iclR"
                     /locus_tag="ACHOQY_003271"
     CDS             complement(272..1096)
                     /gene="iclR"
                     /locus_tag="ACHOQY_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9679319.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1296..4979
                     /gene="metH"
                     /locus_tag="ACHOQY_003272"
     CDS             1296..4979
                     /gene="metH"
                     /locus_tag="ACHOQY_003272"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9679320.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTTSISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5199..6830
                     /locus_tag="ACHOQY_003273"
     CDS             5199..6830
                     /locus_tag="ACHOQY_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9679321.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6921..7610)
                     /gene="pepE"
                     /locus_tag="ACHOQY_003274"
     CDS             complement(6921..7610)
                     /gene="pepE"
                     /locus_tag="ACHOQY_003274"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9679322.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7993..9222)
                     /gene="sorE"
                     /locus_tag="ACHOQY_003275"
     CDS             complement(7993..9222)
                     /gene="sorE"
                     /locus_tag="ACHOQY_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP9679323.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9274..10097)
                     /locus_tag="ACHOQY_003276"
                     /pseudo
     CDS             complement(9274..10097)
                     /locus_tag="ACHOQY_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10108..10905)
                     /locus_tag="ACHOQY_003277"
     CDS             complement(10108..10905)
                     /locus_tag="ACHOQY_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9679324.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10971..11465)
                     /locus_tag="ACHOQY_003278"
     CDS             complement(10971..11465)
                     /locus_tag="ACHOQY_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9679325.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11465..11872)
                     /locus_tag="ACHOQY_003279"
     CDS             complement(11465..11872)
                     /locus_tag="ACHOQY_003279"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP9679326.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11882..12688)
                     /locus_tag="ACHOQY_003280"
     CDS             complement(11882..12688)
                     /locus_tag="ACHOQY_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9679327.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12758..13705)
                     /locus_tag="ACHOQY_003281"
     CDS             complement(12758..13705)
                     /locus_tag="ACHOQY_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9679328.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14053..14925
                     /gene="rluF"
                     /locus_tag="ACHOQY_003282"
     CDS             14053..14925
                     /gene="rluF"
                     /locus_tag="ACHOQY_003282"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9679329.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14926..15198)
                     /locus_tag="ACHOQY_003283"
     CDS             complement(14926..15198)
                     /locus_tag="ACHOQY_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9679330.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3642 a         4000 c         4016 g         3647 t
ORIGIN      
        1 aacagtgaaa aaatttaata tgattacaac taaagaaata tcataaatcg ctcaatctca
       61 taatgcagcc gtaaaagttt cggtggaatg agatcttgcg attttgttaa tgattaagta
      121 gtttgatgtt taaaaataat gctgagttaa taaaagtgtg tgaagtgtat gacattaagt
      181 tattttggcg tcaatgcgat taacagacac ccttattcta ttgccactca ggtatgatgg
      241 gcagaatatt gcctctgccc gccagaaaaa gtcagcgcat tccaccgtac gccagcgtca
      301 cttccttcgc cgctttaatc accatcgcgc caaactcggt cacgcggtca tcggtaatac
      361 gtgaaatcgg tccggaaatt gaaattgcgg caaacggttc gcggtgctca tcgaaaatac
      421 acgctgcaag gcaacgtagc cccagtgcat gttcctcatc gtcaaatgaa taaccccgtt
      481 tgcgcgtttg ggcgagatct tcttttaaat gcacaggaga caccagcgtt gcgtgggtat
      541 aggcatgtaa ccctttgcgg tgcagcagct tcgtcacctg ttcttcgctc agttgggcta
      601 aaaaggcttt acccgcaccg gaagcgtgca tcggcaattt accgccgata ggcgcggaca
      661 ttcgcatcag atgcgtacac tgtacctggt cgataataat cgcttcgtga tcgctttgat
      721 caagcaccgc catattgacc gtttcgccag actcttccat taaattgcgc aggatagggt
      781 gaacaatcgc taacaaatta cggctctgga gaaagctgct gccgaccata aaggcatgtg
      841 cgccgattgc ccaatgtccc agttcgccga cctgacgcac gaaaccctgc tgttgcatcg
      901 tggttagcag gcggtgggtc gtggaattgg gtaacccggc ttgttgcgcc agctctgtga
      961 gtgccacact gccattggat tcggcaatcc actccagtaa tttcaggcca cgcgttaaag
     1021 actgaacctg tccagtcgct ggtgcggtgg caacggcggg ttttctgccg cgtttcgcgg
     1081 gaatgggtgc gaccatgaca gcctcctttt tctgtatcgt ggaaatcatt ttcattttta
     1141 ttgttagcta atgcaatagt tgctgaactg atccgatgag ttaatgttga acaaatctca
     1201 tgttgcgtgg tggtcgcttt taccacagat gcgtttatgc cagtatggtt tgttgaattt
     1261 ttattaaatc tgggttgagc gtgtcgggag caagtgtgag cagcaaagtg gaacaactgc
     1321 gtgcgcagtt aaatgaacgt attctggtgc tggacggcgg tatgggcacc atgatccaga
     1381 gctatcgact gaacgaagcc gattttcgtg gtgaacgctt tgccgactgg ccatgcgatc
     1441 tcaaaggcaa caacgacctg ctggtgctca gcaaaccgga agtgatcgcc gctatccaca
     1501 acgcctactt tgaagcgggc gcggatatca tcgaaaccaa caccttcaac tccacgacca
     1561 ttgcgatggc ggattaccag atggaatccc tgtcggcgga aatcaacttt gcggcggcga
     1621 aactggcgcg cgcctgtgct gacgagtgga ccgcgcgcac gccggagaaa ccgcgctacg
     1681 ttgccggtgt tctcggccca accaaccgca cgacgtctat ttctccggac gtcaacgatc
     1741 cggcattccg caacatcact tttgaccagc tggtggcggc ctatcgcgaa tccaccaaag
     1801 cgctggtgga aggtggtgcg gatctgatcc tgattgaaac cgttttcgac acccttaacg
     1861 ccaaagcggc ggtttttgcg gtgaaaacgg agtttgaagc gctgggcgtt gagctgccga
     1921 ttatgatctc cggcaccatc accgacgcct ccgggcgcac gctctccggg cagaccaccg
     1981 aagcatttta caactcattg cgccacgccg aagctctgac ctttggcctg aactgtgcgc
     2041 tggggcccga tgaactgcgc cagtacgtgc aggagctgtc acggattgcg gaatgctacg
     2101 tcaccgcgca cccgaacgcc gggctaccca acgcctttgg tgagtacgat ctcgacgctg
     2161 acacgatggc aaaacagata cgtgaatggg cgcaagcggg ttttctcaat atcgtcggcg
     2221 gctgctgtgg caccacgcca caacatattg cagcgatgag tcgtgcagta gaaggattag
     2281 cgccgcgcaa actgccggaa atccccgtag cctgccgttt gtccggcctg gagccgctga
     2341 acattggcga agatagcctg tttgtgaacg tgggtgaacg caccaacgtc accggttccg
     2401 ctaagttcaa gcgcctgatc aaagaagaga aatacagcga ggcactggat gtggcccgcc
     2461 agcaggtgga aaacggtgcg cagattatcg atatcaacat ggatgaaggg atgctcgacg
     2521 ccgaagcggc gatggtgcgt tttctcaatc tgattgccgg tgaaccggat atcgctcgcg
     2581 tgccgattat gatcgactcc tcaaaatggg atgtcattga aaaaggtctg aagtgtatcc
     2641 agggcaaagg cattgttaac tctatctcga tgaaagaggg cgtcgacgcc tttatccatc
     2701 acgcgaagct cttgcgtcgt tacggtgcgg cagtggtggt aatggccttt gacgaacagg
     2761 gacaggccga tacccgcgca cggaaaatcg agatttgccg tcgggcgtac aaaatcctca
     2821 ccgaagaggt tggcttcccg ccagaagata tcatcttcga cccgaacatc ttcgcggttg
     2881 ccaccggcat tgaagagcac aacaactacg cgcaggactt tatcggcgcg tgtgaagaca
     2941 tcaaacgcga actgccgcac gcgctaattt ccggcggcgt atctaacgtt tctttctcgt
     3001 tccgtggcaa cgatccggtg cgcgaagcca ttcacgcagt gttcctctac tacgctattc
     3061 gcaatggcat ggatatgggg atcgtcaatg ccgggcagct ggcgatttac gacgacctgc
     3121 ccgctgaact gcgcgacgcg gtggaagatg tgattcttaa tcgtcgcgat gatggcactg
     3181 agcgtttact ggagcttgcc gagaaatatc gcggcagcaa aaccgacgac accgctaacg
     3241 cccagcaggc ggagtggcgc tcgtgggaag tgaataaacg tctggaatac tcgctggtca
     3301 aaggcattac cgagtttatc gagcaggata ccgaagaagc ccgccagcag gctacgcgcc
     3361 cgattgaagt gattgaaggc ccgttgatgg acggcatgaa cgtggtcggc gatctgtttg
     3421 gcgaagggaa aatgttcctg ccacaggtgg tcaaatcggc gcgcgtcatg aaacaggcgg
     3481 tggcgtatct cgaaccgttt attgaagcca gcaaagagca gggcaaaacc aacggcaaga
     3541 tggtgatcgc caccgtgaag ggcgacgtcc acgacatcgg taaaaatatc gttggtgtgg
     3601 tgctgcaatg taacaactac gaaattgtcg atctcggcgt tatggtgcct gcggaaaaaa
     3661 ttctccgtac cgctaaagaa gtgaatgctg atctgattgg actttcgggg cttatcacgc
     3721 cgtcgctgga cgagatggtt aacgtggcga aagagatgga gcgtcagggc ttcaccatcc
     3781 cgctattgat tggaggcgcg acgacctcaa aagcgcacac ggcggtgaaa attgagcaga
     3841 actacagcgg cccgacggtg tatgtacaga acgcatcgcg caccgttggt gtggtggcgg
     3901 cgctgctttc cgatactcag cgtgatgatt ttgtcgctcg tacccgcaag gagtacgaaa
     3961 ccgtacgtat tcagcacggg cgtaagaaac cgcgcacacc accggtcacg ctggaagcgg
     4021 cgcgcgataa cgatttcgct tttgactggc aggcttacac gccgccggtg gcgcaccgtc
     4081 tcggcgtgca ggaagttgaa gccagcatcg aaactctgcg taattacatc gactggacac
     4141 cgttctttat gacctggtcg ctggccggga agtatccgcg cattctggaa gatgaagtgg
     4201 tgggcgttga ggcgcagcgg ctgtttaaag acgccaacga catgctggat aaattaagcg
     4261 ccgagaaaac gctgaatccg cgtggcgtgg tgggcctgtt cccggcaaac cgtgtgggcg
     4321 atgacattga aatctaccgt gacgaaacgc gcacccatgt gatcaacgtc agccaccatc
     4381 tgcgtcaaca gaccgaaaaa acgggattcg ctaactactg tctcgctgac ttcgttgcgc
     4441 cgaagctttc tggtaaagca gattacatcg gcgcatttgc cgtgactggc gggctggaag
     4501 aggacgcact ggctgatgcc tttgaagcgc agcacgatga ttacaacaaa atcatggtga
     4561 aagcgcttgc cgaccgttta gccgaagcct ttgcggagta tctccatgag cgtgtgcgta
     4621 aagtctactg gggctatgcg ccgaacgaga acctcagcaa cgaagaactg atccgcgaaa
     4681 actaccaggg catccgtccg gcaccgggct acccggcctg cccggaacat acggaaaaag
     4741 ccaccatctg ggagctgctg gaagtggaaa aacataccgg catgaaactc acagaatctt
     4801 tcgccatgtg gcccggtgca tcggtttcgg gttggtattt cagccacccg gacagcaagt
     4861 actacgcggt ggcgcaaatt cagcgcgatc aggttgaaga ctatgcccgc cgtaaaggta
     4921 tgagcgtctc cgatgttgag cgctggctgg caccgaatct ggggtatgac gcggactgat
     4981 tcacaaatct gtcacttttc cttacaacaa acagggcgct caatgagtgc cctgtctctt
     5041 tattaatatg aaacacttat actggaaaca ggctggaata atcttagccg gaaaaacgaa
     5101 tgtcaaaagg cacagtaaag atgccagtca gcgtgtttcc ggtcctttgg tcattcaatt
     5161 gcttcaggaa catataacga taaggagaac ctgaactcgt gttaacgctg cttcacctgc
     5221 tttctgccgt cgccctgctg gtctggggga ctcatattgt tcgaaccggc gtaatgcgcg
     5281 tcttcggcgc gcgtttgcgt actgtcctta gccggagcgt cgaaaaaaag ccgctcgcct
     5341 tttgcgcggg gatcggcgtt accgcactgg tgcagagcag taatgccacc accatgctgg
     5401 tgacctcgtt tgtcgctcag gatctggtag ccctcgcacc ggctctggtc attgtgctgg
     5461 gggcagatgt cgggacggcg ctaatggcgc gtattctcac cttcgactta tcctggctgt
     5521 caccgttact tatttttatc ggcgtgattt ttttcctcgg acgcaaacag tcacgcgccg
     5581 ggcaactggg ccgcgtcggt attggtcttg ggctgatttt actggcgctg gagttgattg
     5641 tgcaggccgt aacgccgatc acccaggcaa acggcgttca ggtgatcttt gcctcgctga
     5701 ccggcgatat tctgctggat gcgctgattg gcgcgatgtt cgccattatc agctactcca
     5761 gccttgctgc tgtactgctg actgcgactc tgaccgccgc aggcattatc tccttccccg
     5821 tggcgctctg tctggtgatt ggtgctaacc tcggttccgg cctgctggcg atgctcaaca
     5881 acagtgccgc caatgccgca gcccgccgtg tcgcgctggg tagtctgctg tttaagctgg
     5941 tgggtagcct gattatcctg ccgtttgtcc atttgctggc agagacaatg gggaagctgt
     6001 cattgccaaa agcggaactg gtgatctatt tccacgtctt ctacaacctt gtacgttgcc
     6061 tggtcatgct gccatttgtt gacccgatgg cacggttttg caaaacgatt attcgcgatg
     6121 aaccggaact ggatacccag ctacggccta aacatctgga tgtcagcgcg ctggatacgc
     6181 ccacgcttgc tctggcgaac gccgcgcgcg aaaccctgcg cattggcgac gccatggaac
     6241 agatgatgga agggctgaat aaagtgatgc acggcgagcc acggcaggag aaagagctgc
     6301 gtaagctggc agatgatatc aacgttctct ataccgccat taagctgtat ctggcgcgga
     6361 tgccaaaaga ggaactggca gaagaagagt cgcgtcgctg ggcggagatt attgaaatgt
     6421 cgctcaacct tgaacaggct tccgatatcg tcgagcgcat gggcagcgaa attgccgata
     6481 aatcgctggc agcacggcgg gcattttcgc ttgatgggtt gaaggaactg gatgcgctct
     6541 atgagcaatt gctcagtaat ttaaagctgg caatgtcggt gttcttctct ggcgatgtca
     6601 ccagcgctcg tcgtttgcgt cgcagcaaac atcgttttcg cattcttaat cgccgctatt
     6661 cccacgccca cgtcgatcgc ctgcatcagc aaaacgtgca gagcattgaa accagttcgc
     6721 tacatttagg cttactggga gatatgcagc gcctgaactc gctgttttgt tcggtggctt
     6781 acagtgtgct ggaacagccg gatgaagatg aaggacggga cgagtattaa tattgtgaat
     6841 cctgtaggcc tgataagacg cgtcaagcgt cgcatcaggc actatgaaca agatgccgga
     6901 tcatgcatcc ggcaatttac ttaaaaacgg tgacctgctt ctagcggcac cgcttcttca
     6961 ccagccttaa acacataagt ggtgttcggg ccacccagcg tagcgtgacc tttactcact
     7021 gtgatccagt taccttccgg tagaccaata atcgtcagtt ctggcgcgac gaccagcagt
     7081 tcgcgaatac gctgctcacg ggtttcacct ttatggcctt ccggcagcgc gttggtgaag
     7141 tgcgggttga tttgcagcgg gaacagattt agcgcatcga aaccttgcgg atcgacaatc
     7201 ggcatatcgt tggtggtacg aatggttggg caagcaaggt tagcgcctgc gctccagcca
     7261 atatacagcg caccacgttt aaccacgtca gtaattggtg ccagcagccc gcgctcgcgg
     7321 cactgtttca gcaactggaa agtattcccg cccccgacaa tcacgatctc agcattttca
     7381 atcgcggcaa cgggatcgac aacgctatga ataccggtga cagatacacc cagcggagcg
     7441 agaaccgcag ccgttttcgc tgtgtaatca tcccaggtct gcgttacgcc agcgaaagga
     7501 ataaataccg ctgaacggcg accctgcaac tgttcagcaa tcagcggcag cgcatgttcc
     7561 agccaggctt tacccggcag cgtcgagtta ctcaataaaa gcagttccat tacttctcca
     7621 gttagtaaga aatcacggga ggcatgctac cactgcgaat ttaaccactt actgacatgg
     7681 ctcacgatgt cacgtattgc accgggcgct gagactgatg acaaacacag cattgcctga
     7741 tgcgctacgc ttaccaggcc taccagataa ctgcaatata ttgaattttc gagattatgt
     7801 aggccgaata aggctttatt ccgcatccgg cataaacaat gtgtactttg ccaccaattt
     7861 gagcgcccct taaggcgctg atgttttttt atccttctcg tgcacattgg gccgcttcag
     7921 caatcagcca cacatcaggt cgcgaagttg ccgcggcgac cagccagcga catgcagagt
     7981 taagtagcgc gattaatcat gggaaattgc ctctgcatga gtgagcagat attgctccgc
     8041 ctcaccggac cagatcccct gatgacgcgc cagaatcgcc gccagtgctt gatcctgaat
     8101 ctgcgtgagt gacgtcagcg gcaggtattt cccggtatac accagctttt tgccgccgcc
     8161 gacggcaggc aattcaagcg tggtttcgcc cgcggcattc agcccaagaa tatgtgttac
     8221 cacttttgcg gcctgcactt ttttctcttc aatcagtttg accgccgcgc gcatgtcgtc
     8281 ggtattgccg cctgacgtgc ccacgtagtg ggtaaatgca taatgcacat cgtagaaatt
     8341 aattggcgcg ctgaaatgtt tatcctgcgg tccggcgaag aaattcaggc aaccatctgt
     8401 cgccagcaag gaagaggcga gagtcaccag tccttcatta ggcacaaaga cgaaaatatc
     8461 atcgaagccg tgaccgccac tcagcgccat tagcgtatca aatgctgcat cggcggcatt
     8521 gagataatga atcagtgttt gcggttctga tggatagtgc ttgcgcgcat aactcaattt
     8581 atcgttgtcg gtatcggtaa tgacgagcag cgacgggtta acgggtccat gtagcgcata
     8641 atcaatcgcc aacagtccca ttggtccggt gccgccgagg atcagcatgc gcccttgcgg
     8701 gcgaatcccc atcgtgtggt tataactacc ttcctgaaga tgatagttgg cgttgaacgc
     8761 gccaatcacg caggaaagcg gttcaaccag tgagccttca aaataggttt cgccgtcgta
     8821 tgccagcagg caatcttgtt ccatgacctc gttgggaata accacatgcg tggcttcgcc
     8881 gcccacccac gggaaggagt agccggggca gtccgggcgg tcggggagtt gcaggttggc
     8941 ctgaatcaca taacgctgac ccggctggaa tttgtgctgc cactttttac ccacggccag
     9001 aatatcgccg caaaactcgt ggccgatgat gatggggttg gtcgccacat cgtcgggtac
     9061 ttttttatgg ttttcaccca gattggcctc tttccaggaa gagaggcaca ggctgtcagt
     9121 gaccaccgtc gcgaggattt catcctcctg catttcagga aggtcaaagg tttccaggcg
     9181 taaatcacgt ttaccataaa gacgcagagc tgttgttttc ataaaccacc tcatgttgag
     9241 cgatggcccc ggcagtcgtg ccggggcgaa gaatcaggac aggaagccca gcgcattacc
     9301 gataatccct aagccaaaca gggcgaaaat cagccatacc gggttaattt ttttgttgag
     9361 cagacgaacc ataagcagcg tcagaccgag cgccagcaaa ccagggcaaa gttggtcgag
     9421 aatgttctgc acggtcatgg tgacggtgga gccatcggcg gcatgcgttt gtgaaaccac
     9481 caacggtacg ttgattgacg tccatttggt gaccagcacg cccatcacaa acagtccgag
     9541 aatcgacgcg ccttcggtga gtttttgcag cacattcccg cccatatcgc tgacgatatt
     9601 cacccctttg cgaaagccga gctgtaggcc ataccacttc atcgccagac gcaccgcatt
     9661 gaaaataaag aaaaaaaaag cgggccgagg atgttgccag aaagtgccag agatgcgccg
     9721 agcgcagcgg taatcgggcg cagcgttccc caaaccagtg gatcgccaac ccctgccaat
     9781 ggtcccatca gaccgacttt gatgccgtta atggtaccgt catcaatttc cgcgccgttg
     9841 gcccgcgcct cttccatggc ggcagtgacg ccgatgaccg ggccacatac ggctggcgtg
     9901 gtattgaaga acaccaggtg tcgcctgagc gccgcaacct gatcctcttt taatgggtaa
     9961 agtcgcttga tggcggggat catgtcgtag caaaagccca gcccgtgaat acgttcaaag
    10021 ttaaaggacg cctgttgcag gttggagcgc agaaacatgc tcaccagatc gctgcgtgta
    10081 atttttctct gttccatgat ggctccgtta atcgtcaagt tggtcaaggg cggtggaggt
    10141 ggtggcctgg ggttgaggtt cagctttacg ccactgtgga tttagctgga tgtagaccag
    10201 ggccatgatc acgccgacgc caccgaacgc cagcagactg agatcgaggt agccacctgc
    10261 gaggaaaccg aggaaaaaga aaggcatcaa atatttcacg cccatcatac gtagcaccat
    10321 ggcgtaaccg accaccacga taaaaccgcc agcaatttgc agaccgcgcg tcacgaattc
    10381 cggtatcgcg ctcagcatat tgctcaccat atcggcgctg acgaacagtg agacaatcag
    10441 tgccggaata gcaacgcgca gcgcctgcac gccaagcgcg gagacatgca gaatatcgag
    10501 agtgcgaaac cgcgcttctt ccgctgcttt atccgccgcg tgctggaaca ctacggtgat
    10561 ggtacgggca aacacagtca gcacctggcc tgccgcagcc acaggcaacg cgatggcgat
    10621 tccggtggcg atgctttgct gaccaacgat aaccagaatg gcggagatta tgctggcgag
    10681 tgcagaatcc ggagattgcg ccgcgccgac gttcatccag ccgagagcta tcaattccag
    10741 cgtaccaccg agtattattc cggtttttaa atcaccgaga attaaaccaa tcaccgtaca
    10801 ggcaattaac gggcgatgag tctgaaattc atccagcacg ctgcccattc cggcaataca
    10861 ggaaaaaaga aatatagcaa tgatttgtag ggtacttatt tccatgattt ttcacctttt
    10921 aagtgtcgtc atccctgcct gttaaagcat atgtacacag gcgctattat ttaatttgcg
    10981 attaactgtt cgtttatttt gtcgataata ttgattgagg gatctgacgc tacgacgcgt
    11041 aaatccagta tgacgccaag attatttaac tcatgaaatg cgttaatatc atcatcatct
    11101 aatgaaaccg ctttggttaa ctgtttttta ccgggccgcc aggccatgcc gccaatattc
    11161 agggtgtcta tttttacccc ctggcgaacc atcgctaagg catcctgtgg gcgagtgaaa
    11221 agataaaaaa cggtttcgtc ctgatattgc gggttgtgat aaacagcgac ggctttttca
    11281 atattgacca cgttaacctt cattcccggt ggcgcagcct gacgcaataa tgtccggcga
    11341 acttcatcgt tataaacttc gtcattgcag ataataatgc gctgggcgtt agccactttc
    11401 gaccagacgg tagtgacctg accgtggatc aggcggtcat caatgcgggc gagcgtaatg
    11461 ttcatcagaa atcctcctct gccgttgcca gttgtttcca gacaacgcag gtttgctttg
    11521 cgatttgcgt caggtgttca cacagttcat caacattcat gctgtcctgg ttatcaacca
    11581 gttcgagcgc cagcggcaga gaaaggccgc tgattacccg cagacgagga ttgcgcatcg
    11641 caagcatcgc tgccgcattc cacgggctgc cgcactgtaa atccacggca atcagccact
    11701 catcttgatt gtgaatgctt actaactttt ccagctttgc tacgatgtct ccggcgttct
    11761 cgccgggcac aaattcaacg gcttcaacat tggcgttgcc gtaaaccatc tgaaccgatt
    11821 ccagcatggc gcaagctaat ttgccgtggg cacaaaaaat ggcgttgacc ataaagcctc
    11881 cttagccgcg cgttttgccg cccgcaatgt tagtggttac tccggtgatg tagctggcgc
    11941 gttcagacaa cagataacaa acaaaatcag cgacttctgc taatcttccg gcgcgcccaa
    12001 taggaatggc gtttttggta tagccttcac gcagctgctc gacggtgata ttgcgcgtcc
    12061 acgccagcgc ctcttcatat tccggcgtgc gtagcccggt tttttccaga atcccgggcg
    12121 cgataccgac cacacggata ccgtgtttac ccagctcttt cgaccaggag cgcgtgaagc
    12181 tattaagcgc ggctttggta gcggcgtaac agctttggcc ttctgagcct tccagtccac
    12241 tttctgagga aacattcaca atcacgccat catgttgttt gaccatctgt cgcgccaccg
    12301 cctgcgacat cagaaaaacg cctttctggt tgatattgac cattttttcg aatgcagctt
    12361 cgttgagttc atactgcccg gcaggcgctt tctcatcgac cagcagacgc gggaaattga
    12421 ccccggcgtt attgaccaga ccgtcgatgc gaccaaaacg ctggataatt tccgctaccg
    12481 tatgatttac ctctttggcg ctggaaatat cggtcggcca aaactgatag cctttatgac
    12541 cttcatattg accatcgcca ccgtgaatat ctaccatctg tacgtttgcg ccttgtgcta
    12601 ataattcctc tacaatcgcc agaccaatac cggatgcgcc gccggtgacg ataatgattt
    12661 tatcctgcaa atttaaccac gtttgcataa cagactcctg attttagata atagggaccc
    12721 gccgtaattt ttattacggt aaacatcggg aagattttta tttcagtaat agttcagctg
    12781 tgctgctatt cgttaccaga caattaatat attttccacg cagtgcacca ataattccgc
    12841 tgtatttttc ttcactcatg gcaatgccaa tggaatatcg cgcctgcttt aatttattca
    12901 tttcgataga gagcgttttt tcgctcatat tcgtttcaac cattgcgccg tgaatatcaa
    12961 aaaagcgcga gcaaatatcg ccagcaacct ggcgggcatt caggtcgtca ctctcttcac
    13021 caccataaaa cgcatgccag ttagcgccgt cgcgaatggc cggtgagcca attcccacca
    13081 gggcgacatc cagattatcc cagtaggcag agatggtttt aaagtgctga gactgcatga
    13141 tcccattacg aattaatggg ttatccagca gagccggaaa atccgcgaga tgcgattccc
    13201 ctttcagctt cgctgccgcg ctgtaggtta atgtgtttac atgatagcgg ctttcgagtt
    13261 taccggacgg tccgccaata attggtacgc agattaactg ccgcgattgc cccgcctgcg
    13321 gcaagttttc aaccagtgcg ctcaccgcgc gaccccagga aaaaccgaca atatcgccag
    13381 gctccagcaa gcgatccagc agttgcgcgc cgtgtaaccc catcatcgcc agttgagtct
    13441 cttcatcctc atcatttccc gacaccacca caacgtcttt caggccaaac ttttgcttca
    13501 gttgctgctc cagccagaga ttttcgttgt agtcatagtt gatggcgatg gtgacaattc
    13561 cctgatcgcg gcctcgttta agcaagcggc tgatggtggt gcggtaaata cgcagttcgc
    13621 gcgcgatttg cgcctgcgtc atatcctgtt cgtaatagag ttgggcaatc ttcacaatca
    13681 aacggatatc gtcactgttt tccattgccg cctcctggtt ctgcacattt gtgcaaatac
    13741 gctttctcac cgcttgatct gatataaccc catcatgagg gcctttcaaa gcgatttttc
    13801 actctttgca tggggagaaa tagcgctttc tcttgcacat ttgtttctct gcgtagtagt
    13861 catcagcaca ggtgtgcagc gattcgtaat atgttgatta tccttatttt gccaggcaaa
    13921 tttgcatcgt acttccggag acacttttgt gataaaaggc gtggcagatc acatttaatt
    13981 tcgacgttgc cgcataaccc tgtttttccc cgctaagagg tacggtatat tccgcgtttt
    14041 acaggagaat ttatgctgcc cgactcatca gtccgtttaa ataaatacat cagcgaaagc
    14101 ggaatttgct cacgccgcga agcggatcgc tatatcgagc aaggcaatgt gttccttaat
    14161 ggcaagcgag ccaccattgg cgatcaggtg aaacccggcg acattgtgaa agtaaacggt
    14221 cagttgattg agcctcggga agccgaagat ttggtactta tcgccctgaa caagcccgtc
    14281 ggtattgtca gcaccaccga agatggcgag cgcgataaca ttgtcgattt cgttaaccac
    14341 agcaaacgcg tgttcccgat tggccgcctg gataaagact cccaggggct gattttcctc
    14401 accaatcacg gcgatctggt gaataagatc ctccgtgcgg gtaacgatca tgagaaagag
    14461 tatctggtga cggtcgataa accgattacc gatgaattta ttcgtggtat gggggcgggg
    14521 gtgccgatcc tcgggacggt gacgaaaaag tgcaaagtca aaaaagaagc gccgtttgtc
    14581 ttccgcatta ccctggtgca ggggctgaac cgtcagatcc ggcgcatgtg cgagcatttc
    14641 ggctatgaag tgaaaaagct ggaacgtacg cgcattatga acgtcagttt aagcggcatt
    14701 ccgctgggtg agtggcgcga tttaactgac gatgagttaa tcgacctgtt taaactcatt
    14761 gaaaattcct cttccgaggt aaaacctaaa gcgaaggcta aaccgaaaac agcgggcatc
    14821 aaacgtccag tcgttaagat ggaaaaaacg gcggaaaaag gcggtcgccc ggcgtccaac
    14881 ggtaagcgtt ttacctcgcc ggggcgtaaa aagaagggac gctaatcagc gcctgccgcg
    14941 cgtcgacgta tttgctgacc agctaaatga cgcttcagga tccggtttat aagcctgctt
    15001 tttcttcaac tggcgggctt ttttcgcttc agcctcacgc tcgaccatta atttatcgat
    15061 gtactctttc ttaatgctgt tggtttcgga gttggtcagt acgcgaccat gcgcaatccg
    15121 cgcccgatcc agcagtgttt taagttcacg ctgctcgcgt tcagtcatct ctttttgggt
    15181 aatacggggg agtgccatga tggtgccctc agtgagccag agagttcagt gtacgggaat
    15241 tcgtttgcga gctgaactga taatattcct ttgttctctt cttaaatatt tatctttctt
    15301 gataa
//
LOCUS       DAZKGS010000106        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKGS010000106 DAZKGS010000000
VERSION     DAZKGS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(219..812)
                     /gene="tehB"
                     /locus_tag="ACHOQY_003284"
     CDS             complement(219..812)
                     /gene="tehB"
                     /locus_tag="ACHOQY_003284"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9679331.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(809..1801)
                     /gene="tehA"
                     /locus_tag="ACHOQY_003285"
     CDS             complement(809..1801)
                     /gene="tehA"
                     /locus_tag="ACHOQY_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9679332.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1925..2905
                     /gene="ydcK"
                     /locus_tag="ACHOQY_003286"
     CDS             1925..2905
                     /gene="ydcK"
                     /locus_tag="ACHOQY_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9679333.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2900..3436)
                     /gene="rimL"
                     /locus_tag="ACHOQY_003287"
     CDS             complement(2900..3436)
                     /gene="rimL"
                     /locus_tag="ACHOQY_003287"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP9679334.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3499..3723)
                     /gene="ydcH"
                     /locus_tag="ACHOQY_003288"
     CDS             complement(3499..3723)
                     /gene="ydcH"
                     /locus_tag="ACHOQY_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9679335.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3863..5518)
                     /gene="opgD"
                     /locus_tag="ACHOQY_003289"
                     /gene_synonym="mdoD"
     CDS             complement(3863..5518)
                     /gene="opgD"
                     /locus_tag="ACHOQY_003289"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9679336.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5743..7086)
                     /gene="ydcJ"
                     /locus_tag="ACHOQY_003290"
     CDS             complement(5743..7086)
                     /gene="ydcJ"
                     /locus_tag="ACHOQY_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9679337.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7303..8226
                     /gene="ydcI"
                     /locus_tag="ACHOQY_003291"
     CDS             7303..8226
                     /gene="ydcI"
                     /locus_tag="ACHOQY_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9679338.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8264..9904)
                     /gene="trg"
                     /locus_tag="ACHOQY_003292"
     CDS             complement(8264..9904)
                     /gene="trg"
                     /locus_tag="ACHOQY_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP9679339.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            10303..10452
                     /gene="hokB"
                     /locus_tag="ACHOQY_003293"
     CDS             10303..10452
                     /gene="hokB"
                     /locus_tag="ACHOQY_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP9679340.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(10430..10498)
                     /gene="yncP"
                     /locus_tag="ACHOQY_003294"
     CDS             complement(10430..10498)
                     /gene="yncP"
                     /locus_tag="ACHOQY_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP9679341.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10524..10697)
                     /locus_tag="ACHOQY_003295"
     CDS             complement(10524..10697)
                     /locus_tag="ACHOQY_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679342.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(10942..11472)
                     /gene="cybB"
                     /locus_tag="ACHOQY_003296"
     CDS             complement(10942..11472)
                     /gene="cybB"
                     /locus_tag="ACHOQY_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9679343.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11661..12662
                     /gene="gap"
                     /locus_tag="ACHOQY_003297"
     CDS             11661..12662
                     /gene="gap"
                     /locus_tag="ACHOQY_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9679344.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12704..14143)
                     /gene="aldA"
                     /locus_tag="ACHOQY_003298"
     CDS             complement(12704..14143)
                     /gene="aldA"
                     /locus_tag="ACHOQY_003298"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9679345.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14340..15140)
                     /gene="ydcF"
                     /locus_tag="ACHOQY_003299"
     CDS             complement(14340..15140)
                     /gene="ydcF"
                     /locus_tag="ACHOQY_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9679346.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT         3948 a         3860 c         3569 g         3878 t
ORIGIN      
        1 aaaataaatt tttcagcccc ttttcagacg acatagtcaa tgccgaaaaa ggggctaagt
       61 caatcatcag tagttaaatt tcagtaagtt aagctgctaa aaaacttcga ctactgtgat
      121 taatttcaca tcccttcgat aaaaaatatg cttaccgaaa agaagaacag taaatcatct
      181 cagcgtgggg aattttcacg cgccttattc ttagcgggtc attttttacg tgccagcatc
      241 gtggcgaaac gcagtttaat acgattaccg ttggcgtcgg tgcggtgcag ctcgccgacg
      301 tcttcattgt atttcaccct ctcccagcct tcgtaatatc gacgtaattc tccctctttg
      361 aaggcaaacg ggaagccgac ggtacatgga taatcagcgg tatccatcgc cgccacaatc
      421 aggttgtaac caccaggttt agtgcaacgt tgcatattgg caatcagccc tgggatggtt
      481 ttagcctcaa ggaacatcag taccacagtc gaaagaataa aatcgtactg tctatcaaat
      541 gtgaggttat tcagatcaac gactcgggtg tgtaaattat ccagattttc aatggattta
      601 atgcgctcta cattggcgat actcatggca tttttatccc atgcgtcaac atcataacca
      661 ttggctgcca ggtaaagact gttacgacca ttgccacagc ccagatccag cgttttaccc
      721 ggtttaacca ctttcaccgc ttccagtact tcagagtgtg tgcgggttaa ttcatattta
      781 tcagtaaaat agttttcgtc acgaatgatc attctttgtc ctctgctttc attaaaacgg
      841 cgcgctcggt tctgactaac aattttccct gcatcagaag cgcaaaagta cggatgagca
      901 gtattgcaat aataaaattg gtaaagataa acagcggtac cgccagcgta tggaaaaatc
      961 cattttcgct gccactcccc agatgcaaac cggtggttgc cagtgcagat acgccgaacg
     1021 agaaactcca gaatgaagca ttaaatggct gggagagata ccatggcatc agacgtagca
     1081 taaacagcag ttgcagcagt ccataaccaa aaagcatttt cgccagcgta tcaccctcgc
     1141 cgccgttgac gctcaaccag gcactacagg ccaccagcgc aggagcgagc tgaatgccga
     1201 gtgatgtccg cagtgccgtg ggtaattctc ccgaactgcg cagacgctgc aagatcacag
     1261 gttcaaggct tagccatgag aaaacgcctg cgcctaaaaa caccagaccg gcgtcggtgt
     1321 agcccaacgc accacaggcc attgcgctga taaagttgtt ggcaactgtc ggcagataca
     1381 gtccaggcgt ggtagcttct tcagggtgag atccgcgcca taatcccgca gtttgccagg
     1441 cggcataagc caactgaacc acgacaccaa aactgaacag gcacaccgcc agtgggcgaa
     1501 accacggaac aaaaccaatc gccaccagca tcgtcgttgc cggaaacaaa ctcacaaaac
     1561 tgctcagcac tggatggcga acttccgcca gcacgctatg cggaaagcgt atgagtcggg
     1621 taataaatgc gctagttaat aatccccaga tgatcatcgc cagaatcacc agcccatccc
     1681 ctaaccagtg gctgacctgc caaacctggc tggcatagcg ccaggcaaat cccatcccta
     1741 tcgtccccaa cacaatacca aagtagcctg ccggcaaatt gagcacttta tcgctctgca
     1801 tcttcttact cattttgttt aatttataaa gtatatttga aatgcatttt atggaatttt
     1861 ctcgagcata gccagagccg cagaatttgc tacggttata gtaagtcacc tgtgggaaaa
     1921 cgctatgcgc aaatatcgtc taagtgaaga acagcgagcc tttagttatc aggaagatgg
     1981 cactaaaaaa aatgtgttat tacggcagat tatcgccatt agcgatttta acgatgtgat
     2041 agcaggaacc gctggcggct ggatcgatcg cgaaacggtg ttagcacagg aaggaaattg
     2101 ctggatttac gaccagaatg ctattgcgtt cggcggaacg gtgatttccg gtaatacgcg
     2161 catcaccggc accagcgtac tgtggggaga ggtttacgca acggataatg tctggatcga
     2221 taatagtgaa atcagccagg gtgcatatat aagtgacggt gtcaccattc atgactcact
     2281 ggtttgcggg caatgtagaa tatttggtca cgccttaatt aatcaacatt ctatgatcgt
     2341 cgcagcacaa ggcttaacgc ctgaccatca actcctgcta caaatttatg atcgcgcaag
     2401 ggttagcgcc tcgcgtattg tccaccaggc acaaatttat ggcgatgccg tcatccggta
     2461 tgcttttatt gaacatcgtg ccgaagtttt tgattttgcc agtattgaag gtaatgaaga
     2521 gaacaacgtc tggctatgcg attgcgctaa agtctacggt cacgcgcagg tgaaagctgg
     2581 catagaagaa gatgccattc caacgattca ttacagttcg caggtggcgg aatatgccat
     2641 tgtggaaggt aattgtgtgt taaaacatca tgtcttgatt ggcggaaatg ccgtggtacg
     2701 tggtggaccg attctgctcg acgagcacgt tgtaatacaa ggtgagagcc gtattacagg
     2761 tgcggtgata attgaaaacc atgtggagtt aaccgaccac gccgtcgttg aagcctttga
     2821 tggcgacacg gttcatgtcc gtggtccaaa agtcattaac ggcgaggagc gcattacgcg
     2881 aacgccgctg gcagggttat tatgaatcga taatacgcgc gtataagttc acatcatcat
     2941 aggcatcatt caggaactca gcctgtttca ggcaaccttc aaggataaaa ccattgcgca
     3001 aagcgacctg gttgctttgc ggattgtcca cccgacattt gatcacgaag cgtctaagtt
     3061 caccagactg ggcgtaatga tgaatcaatg cctgcagcgc ctgagaaatg atcccctgcc
     3121 cctgatgaga ttcgtccagc cagtagccta tttcagcggt tttattcagt ggttcaatac
     3181 gattaaacga gataacgccg ataagttcat cttctttgaa aatcatgaac attttggcat
     3241 agccgcgttg atgcaacatc acattaccct gcaccgtttt tcgcgtgtcc tcttcactct
     3301 gaacgaactg cggccagttt agcgactgct gtaaccaggt tttattttta cagattaact
     3361 gataaagagg agtgacgtga ttttctgcaa cagcatgtaa ttcaagtgat tcgcttactt
     3421 ttatcgtttc agtcatctta tctccagtca atccatgcag ttgttaaaaa agccgcctgg
     3481 ctggcggctt aggaaagttt acacctcttt gacgctctcc tgctgcagga ttttgagcat
     3541 ctcatctttt aactgtagct tttgtttttt catgcggacc acttccgcat tgtaccctcg
     3601 accgtcggaa ccttcctttc tggcaatttc atgatcaagt ttattgtgtt tatcgaacaa
     3661 ggacataaag cgaggatttt cgtttttcag acgggatatt aaatctcgat attctggaaa
     3721 catacgcact ccctgttagt ggctgacaag gtgtatctga aatacacacc atcagattac
     3781 tcatttaact tagggaaaaa tgcgagcggg atcgtgttta accagacggc acctcccgaa
     3841 gaaggtgccg aagaaaaaac gattaactca tcacgcggtc gtcaacatac tgacgtttat
     3901 ccggcgctgg cgggaaatac tgatacagcc atgtttcact gatagcgtcg ccctgacaac
     3961 gcagatacat ccgcatatcg accggatcag tggagtccga agtcggatac cagtcaaact
     4021 gaatacgata accatcaatg ggttcaatat agagaatttc gatttgcttc gcttccccac
     4081 tggaaagcgt aatcaccggc tcaatgcctt ttggtgcggc agctttcaga tcaccaccaa
     4141 cgaaatcgac ggcaaaacga cgcgcccatt tttcgggata gtgttcacct ggtgcccaac
     4201 cttccgggaa accgcccatg ccggtacgcg tcgccataac gcgcgctaat gggcaatgaa
     4261 caggcggttg cgcactccag tacagacgat actggaatgc aaactcatca cccgccttta
     4321 cagctttttc tggctgccag aagcagacaa tgttatccag cgtttcgccc gttgttggga
     4381 tttccatcag gccgatggtg cccttacccc acttgttacg cggttccacc cacagacttg
     4441 ggcgtttgtt ataccagccc ataatgtcct gataatggga gaaatcacga tccagttgca
     4501 ataaaccaaa ccctttcggg ttgttgtcgg tgtaagcatt gaactgcaat ttttgcggat
     4561 tattcagcgg acggcaaatc cactcgccgt tgccccgcca catggacaga cgatcagagt
     4621 catgaatttg cggatgaatg gtgtcgcaca tccgacgttc attagtaccg cagctgaaca
     4681 tactggtcat cggcgcaatg cccagctgtt taatgtcttt gcgcgcatac aggtgatttt
     4741 ccacatccat aatcacctga cttttctcac aatggatagt gaacttatag gcaccagtaa
     4801 tgctggcgct atcgagcaac gcataaacgg taaaggtagt tgcccccggt tttaccgtat
     4861 caaaccagaa ggcggtaaag tcggggaact cttctttact gtcggtgtaa gtgtcaatcg
     4921 ccaggccgcg ggccgacaaa ccgtattgat atgtgtcatc aacggcgcgg aaataactcg
     4981 ctccgaggaa tgatactaca tcacggcgcg ccagttcggg ggctttaaac acgcgaaaac
     5041 cggcaaagcc gagatcgctt tgcccttcta attgtttggt atcaacacct gcatcgttgt
     5101 atttgaacaa ctccgggcga aagtgaattt cacgcgccag atgtgttgct ggatctacag
     5161 aaaacatacg aacgcggcga cggaatccca ttcccatatg gaagaactga gcgtccagtt
     5221 gacggttctc aacgttatgc cagagcgatt tttcggcgtc gtattgaata ctgttataag
     5281 cctgcggcgt cattgtcgcc agcgtgtcag gtaacggacg aggcgcacca cgccacgctg
     5341 tttgcgctaa gtcgtgcgcc attgactgta gaatggagaa gtcaaaacgc tgggtttgcc
     5401 cgtcggcaat atcagaatct gccgcgaatg ccgcctgaga aaaaagagaa gcaatgccgc
     5461 tggtaccgca cacggcggcc atagccattg aacctttaat aaatcgtcta cgatccatac
     5521 ctgaaagtga gtccttctgg tgttgtgaat ggtcccgcgc tggtcgtttt cagcggcaag
     5581 aaagtgctta tttatgaccg ctcactctag acaatattta ttaataatcc aattgttgaa
     5641 ctcagagaat atgcataaat cggcaatttt tttatgtcga tgggatgagc ctgattcgtt
     5701 tgtaaaatca ggcaaatttt tgcaaaggcg gatggtcaga atttaaagca aaccacagcg
     5761 acgtttactg cgttcttccg cttcctgata aagctggaac tcatccaaaa ccggacaacc
     5821 caacgcctgc tcaaatgcct cgcgactggc attaccgtga ttgcgtgcct gcgtttcatt
     5881 acccagattt gactggaaga tccccgccgc gctaacgggc aagaaatctt cataggtaat
     5941 gggttgcgcc gcgacccaac cacgttcaat taagggctgt ggatcgtctc cgggatgaat
     6001 cgcctgacga tgcgcctcac ctgaaggcgt cagacggtac cggaaccatg ccagcccttg
     6061 ctgacgcatt aaaaactcac tgtcaggaaa agtgcggaag gtttcctgta aatgcatttg
     6121 gtgagtgaga ttatcctgcc cggttccagc gttacgcaga agatcatcat acagttgtcg
     6181 ccctttcggc gttaatgcca cgccacgctg ctcaatttca ccaaagcgcg cggtatgcgt
     6241 gccctgtttc tgccccgcaa acaacaccgt ctcttccagt gctttaaagc tggtctggcg
     6301 tagtaaaatc ggtacctcgc ggcgcggcgg cccctcgatg agaattttgg gttcaattcc
     6361 gcattcaggc atcatcgact gcacccggtc aatatccagc gtacgtggcg tcaggtggtt
     6421 gatatggcat ccaggaaaac agaccacatc agcaatcaac cgatgttcgt tgtgcaaggc
     6481 gcgataggtt tcttcatcta ccgttgctga ctggtgccag cgaaacgttt ccagggcttc
     6541 ctgcacaaac tcctgtgcct gtgtttcgtt aaaaccgccc tgctgctcat attcctctaa
     6601 cagttgtcga caacgtgggg tgaagatatc gcgctgacgc agaatctccg ccgctttctg
     6661 gcgcaaaatt tcgttctcga taagctcaag gcggagtaag gaggtaaaaa cgcggaaggg
     6721 attacgcgcc agagaagcat catcaatggg ccgaaatgct gtcgaatgta ccggcacccc
     6781 tgcctgcgag agatcgtaat agctcaccgg gtacatcccc ataatggcaa acatccgccg
     6841 aagagtagca agctcttgtg cagtcccaac gcgaatcgcc ccatgacgtt caacattcag
     6901 tcgcgccagc tcgtctgcat ttaccatttt ttcgtgcagt tgaggattgt tttccagcac
     6961 agccagattc acatcagcta ccagttccag cagcgtgcca tattgcggaa cttcttgctg
     7021 gtacatggct gacattgcct gcgaaaactg ttcccgaatc tcatccgccg tgatgctgtt
     7081 cgccatagtg tctgcctcca gtggtcagta atctggagtg taggtaaccg cattcactct
     7141 ggcgggaaga atttacaaac tgtgatctcg ccgcgaaaac atcaatatta tccattttgc
     7201 tgtaacacaa ttgctttaat tgttaataat attttgcaat caagttatca taatcaaaca
     7261 acttcacttg tcagcgacac cgcttcgttt ttaacatcgc ttatggaaaa aaatagtctg
     7321 tttagtcagc gcatccgttt gcgccacctt catacattcg tagctgtcgc acaacaagga
     7381 actttggggc gcgcggctga aacccttaat ttgagtcaac ctgcgctctc taagacattg
     7441 aatgaactgg agcagctgac tggcgctcgc ttgtttgagc gtggtcgtca gggggcgcaa
     7501 cttaccttac ccggcgaaca atttttaacg catgcagtca gagttcttga cgccatcaac
     7561 actgccggac agtcgcttca tcgtaaagaa ggtcttaata atgatgtcgt cagggttggt
     7621 gcactaccta ctgcggcact ggggatatta ccttcggtta taggtcagtt tcatcagcaa
     7681 caaaaagaga cgaccttgca agttgcgaca atgagtaacc ctatgattct ggcgggttta
     7741 aaaaccgggg aaatcgatat cggcattggt cggatgtcag atcctgaact gatgaccggg
     7801 cttaattacg aactgctgtt tcttgaatcg ttgaagctgg ttgtccgccc taatcacccg
     7861 ctacttcagg agaacgtaac gctaagccgg gtgctggaat ggccggtcgt tgtatcacca
     7921 gaaggcactg cgccacgcca gcattcagat gcattagtgc agagccaggg ctgtaaaatt
     7981 ccttcgggtt gtatcgaaac gctgtctgct tcgctatctc gtcaacttac ggttgaatac
     8041 gattacgtgt ggtttgtccc ttctggcgct gtaaaagacg acctgcgtca tgccacgctg
     8101 gtggccctgc ctgttccggg acatggtgca ggcgaaccga ttggaatact gacccgcgta
     8161 gatgcgacgt tctcttctgg ttgccagttg atgattaacg ctattcgaaa atcaatgccg
     8221 ttctgaaagg tgaagggatc tgtcgatccc tccttgaaca ttttcacacc gtagcgaaac
     8281 taactggttc acccgctccg cgaggttctg ccgacacaga atgtttgtgc agacggaata
     8341 catccaccgc ctcagttaat cgtgccgcct gttcttcaag ggacaccgcc gccgctgagg
     8401 cctcttctac cagagaagca ttctgttgcg tcaccttatc catttcagaa atcgcctggc
     8461 taacctgcgt tatgcctcta ctttgttcat ccgaggcggc ggcgatttcc tgcatgatat
     8521 gtgtgacact cgcgacggca tcaacaatag tgctcatggt tttccctgcc gttgccacct
     8581 catccgaccc caggtcaatt aacctgactg attcactgat caagccttca atctctttcg
     8641 ccgcttgggc gctgcggctt gcgagtgtcc gtacttcgct ggcgacaacg gcaaatccac
     8701 ggccttgctc acccgctcgc gcggcttcaa cggcagcatt cagtgccaga atattcgtct
     8761 ggaaagcaat actgttgatg acggcggtga tctcggaaat tttcttcgaa cttgtagaga
     8821 tagcgcccat cgtttttact acaccggaaa ccgtctgccc gccatcgctg gctttaatag
     8881 aagcctcttg cgccagtttg ctggcatgat gcgcgttatc cgcattctgt ttcaccgtcg
     8941 cagtgagttg ctccatgcta gcggcagttt gttcgatagc cgccgcttgt tcttcggtgc
     9001 gagatgacag gtccgcattg ccagctgaaa tttcgctggt gccacgataa atctcttccg
     9061 caccctgtcg aacagtccct actgtcatcc ccagtgaatg ctgcatttgc tgtaaatgac
     9121 gacttaagcg accgatttca ttacgacccg ccggttcatc attcatcgtc agatcgccac
     9181 tggcgatttt ttcaatccgt tgtgcggcat gttgcagtgg acgaatgacg atccgacgta
     9241 gcaccataaa tgttatcagc gtcatgacca gggcaagcac aaacgcgcca atcatgaaca
     9301 tcccacccag gcgggtgcgc tgatgggcca gttccgccag ttggttggct ctggtgctac
     9361 gtatcttaac ggctttgttc aaaatatcgg tataagcatt atccagcggt cggatctgct
     9421 cactttcatg attgataatc gcttcaaaca tgccattttt ggcatatttc aacataggtt
     9481 gcatacccgt gatataagcc tgaaagcgtt gatttaattc agtgtcgagg gcttcatcag
     9541 caggtgtttt caccggtcga ttctgataag cacgataacc ttgctgcgac tgtttaatct
     9601 ccgattcggc ttgcgcaata tttcgcttca ttgcttccat ttccgcaata cgacttgcag
     9661 ccccggcctg aatcatattg atccgggctg aacgcaaaaa atctgaactg ttcgctaacc
     9721 cggtccgaat ctcaatctcc gcagtaacat cacgctgatc gcgatcggcc tgccagagga
     9781 aatagccagc cagagcagaa cttaatgcga atagaactaa gataccgcca agaatgcagg
     9841 caaataacgg aaccagcctg atgtgatgca aaaaacctaa tcgatgtgag ggagttgtat
     9901 tcattaccgt tgtctctcgt ccaggtttac tcctgataga aagtcatcgg cacttgacgg
     9961 taattactta tgaccaaaaa cctctttgcg tcacattttt acaacaatta ttgcagagaa
    10021 aattaatgct attggaatga agagagtaac gaatggctaa actggctaac ccgaatgacg
    10081 aaaaattctg gaaaacagca cgcaatttcg tgaagtgatt attttcgtcc ctaaggaaac
    10141 ctcataggat gcctgcgggt gcctgagact ttctgttttc aggctaaccg ggaatcagaa
    10201 agacgaaggc cccgaccgat atttctatca accgaggcca acgaatgaac ctagcaattc
    10261 agatcttagc ctcttacccg ccatccggca aggagaaagg ctatgaagca caaccctctg
    10321 gtggtgtgtc tgctcattat ctgcattacg attctgacat tcacactcct gacccgacaa
    10381 acgctctacg aactgcggtt ccgggacggt gataaggagg ttgctgcgct catggcctgc
    10441 acgtccaggt aagggcaagc gcggggattt tccccgcgca tctgactagc agattcattt
    10501 tctcatggca ccccttttac acttcagtta gtgcatgttt ttttcgaggc actaatagaa
    10561 ccatcattac agacaaattt gccgcctgcc gtacagtgtg agatcccccc tttcgaacca
    10621 gaacagggtt tgcgcccggc atcggcataa aaggaaacaa gcgttcccat aaacaaaata
    10681 agtgccagtt ttttcatata acctccatgg tttatcgaat cttatgttcg atttgaacgt
    10741 tatacgctgg tttattttga taccgttgat gagatcaaag atcgggtgaa ttgattaaaa
    10801 ataagccaga tggacgtatg ggcaaggatt atggttttat ttgtcataca aataaatata
    10861 ataggcgctt ccgatgtaga cccgtattct tcgcctgtac cacgggtcgg ttttagtaca
    10921 ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc atcatgcgta gaagtgtgtt
    10981 gtccttccag aaatagtggt gtgccagtgc agccgcagcg tgcaacccga tgacaaaata
    11041 tcccagattc gccagcgtct catgccacga ctttaagcta tcaacccgtt cgaaattggc
    11101 ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac gggttgcccc ggttatacat
    11161 catcaccaaa ccaatcactg gcagcgcaat aaacaggaga taaatcacca aatgtcccaa
    11221 atgcgccagt cccgtcatca tcggttttgg cttaggtata atcggcgggg ttgggtattt
    11281 cagtcttaac aaaagacgaa ccaccatcag cacgagaatt gagataccac aggaaacatg
    11341 aaccatgttg atgagtggcc gatcgctacg tgggaagaaa ccacgaaact ccattgcgca
    11401 atacgctgcg ataaccagca aaaagaccag ccagtgaatg ctgatttgta accttgagta
    11461 tttattttcc ataacatttc ctgctttaac ataattttcc gttaacataa cgggcttttc
    11521 tcaaaatttc attaaatatt gttcacccgt tttcagtacg ttacgtttca ctgtaagaat
    11581 ggagattgcc catcccacaa tcctggtcta agcctggaaa ggatcaattt tcatccgaat
    11641 gttcctgaca ggagaaaacc atgagtaaag ttggtattaa cggttttggt cgtatcggtc
    11701 gactggtgtt gcgtcgatta cttgaagtca aaagcaacat agacgttgtc gctattaatg
    11761 atctcacttc cccaaaaatt ctcgcctacc tgctgaaaca tgattcaaac tacggaccat
    11821 tcctctggag cgttgatttt acggaagatt cacttatcgt tgatgggaaa agtatcgcgg
    11881 tttacgccga aaaagaggct aaaaatattc cgtggaaagc gaaaggtgca gaaatcattg
    11941 tcgaatgtac tggcttttat acctccgccg agaaatcgca ggcgcatctt gatgctggtg
    12001 cgaagaaggt gttgatttcc gcccctgccg gtgaaatgaa aactatcgtt tataacgtca
    12061 atgacgacac tctggatggc aacgacacca ttgtttccgt ggcgtcatgc accactaact
    12121 gtcttgcgcc gatggccaaa gccttgcatg acagtttcgg gatagaagtc ggcacgatga
    12181 cgaccattca tgcctatact ggcacccagt cactggtgga tggcccacgt ggtaaagatt
    12241 tacgtgcttc acgcgcagcg gcagaaaata tcattcccca cactacgggg gcggcaaaag
    12301 ccattggtct ggtgatcccg gaactgagcg gcaaactgaa aggtcatgcg caacgcgtgc
    12361 cggtgaaaac aggttcggtc actgagctgg tgtccattct cggaaaaaaa gtgactgccg
    12421 aagaggtgaa taacgcactt aaacaagcaa ccaccaataa cgagtcattt ggttataccg
    12481 atgaagaaat agtctcttcc gatatcattg gcagccattt cggttcggtg tttgatgcca
    12541 cgcaaacgga aattaccgcc gtgggcgatt tacaactggt gaaaacggtc gcctggtacg
    12601 ataacgaata tggcttcgtc acgcagctta ttcgcaccct cgaaaaattc gctaaactct
    12661 gacgcgcaca ggcggaggaa aaaacctccg cctcttttac tcattacgac tgtaaataaa
    12721 ccacctgggt ctgcagatat tcatgcaggc catgtttacc atctgcgccg ccaataccgg
    12781 atttacgcca tccggcgtgg aagccttgca tagcttcgaa gttttcacgg ttgatgtaag
    12841 tttcaccaaa cttcagccct ttaatggctt tcatcgcgac gttcagattt tgggtataga
    12901 ttgatgaggt caggccgtaa tcactgtcat tagccattga gatagcatct tccagcgtgt
    12961 caaatgcgac caccggcaga accgggccaa aggtttcctc atgcataatc gacatttcct
    13021 ggcgaacatc cagcagcaat gtcggcggat aataatatcc tttcccgtct actgctttgc
    13081 cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc cactttttgc tcgacccttt
    13141 ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc gttgcgttca gcggggttac
    13201 caaattgaac cgcctgcatc gcttcaccca gccgattgac gaactgatca taaatgcctt
    13261 tctgtacata aacacgttct gcacagttac acacttgccc actattaatg acgcgtgaat
    13321 caacgatggc tttgactgcc agttcaagat cggcatcgtc cattacgata gctggtgctt
    13381 taccacccaa ttccaggcac actttggtga tgtttttcgc cgcagtcgcc ataatcttct
    13441 cacctgcaga gacgctgcct gtcatactga ccattgcgac ctttgggtta cccgccagtt
    13501 cttgcccaac ggtttcacca cgccccagta caaggttaaa cacgccgcgc ggaaggccta
    13561 tttcatcgac gattttggcg aatgcaatcg cattgtttgg cgtaaattca ctgggtttaa
    13621 tgacgatggt attaccggtc aaaagagcgg gagccatttt gcgggcaatg aggaagaacg
    13681 ggaagttcca cggcagaatg ccggtagtca caccaagcgc acgtttaaac aaaagaatat
    13741 tttctcctgg acgatcgctt tgaataatct cgccctcgta acgccgtgcc cactccgcca
    13801 tgtaatcgat atagtcggca gtaaaagcca cttcgacttc agccagctgc tggatcttgc
    13861 ccccttcttc aacaatcagc gcactgattt cactggcgcg ttcgcggatg ccggcggaga
    13921 ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc ttcccattct ggttgtgcac
    13981 gttctgctgc atcgattgcc ttacgggcat cctcggcctg accatcgggt atgcgggaaa
    14041 tgacagcctc tgcagcaggg tttaccacat caatccatgc gtctccacgc caggtaacaa
    14101 actgtccatc gatatacata ggatgttgaa cgggtactga catgggcgac tcctgtgatt
    14161 tatatgtttg ttttcggtga atgcattgtt aattaacatc aatcatcaga gagacggcat
    14221 cgtgaaagga caattctgtg aagggcttca taaaaacaag tgtaaaagga ataaaaggca
    14281 gaaataaaag tcaggagtca tgcccgtttg caaacaagtg gttcacaaac gggcaatttt
    14341 taacgtaacg agcgactttc catcgcctcg atgagcaccg catcatgttt cagcgtttgc
    14401 catgcatgga tgacttctgc cggaaaatca acgtgaacga taaaatctcg cccgcgggga
    14461 ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa
    14521 cgctcaaccg gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt
    14581 cctaactgag gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca
    14641 gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata
    14701 gccgtatgaa ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca
    14761 ccgcagtttg ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga
    14821 tgagcgatat ccgccaggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag
    14881 tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta
    14941 atcagtaaag gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata
    15001 accgcattgc ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca
    15061 tcctgcgcca gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc
    15121 ggaaacgggg taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat
    15181 aatagtgaga agcggagcac tctgaaagga ggaagtcgta tgtccttaac cgccactccg
    15241 caagcctgac atatc
//
LOCUS       DAZKGS010000107        15202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKGS010000107 DAZKGS010000000
VERSION     DAZKGS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..150
                     /locus_tag="ACHOQY_003300"
     CDS             <1..150
                     /locus_tag="ACHOQY_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9679347.1"
                     /translation="AELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLA
                     GSGLS"
     gene            419..2656
                     /gene="nrfB"
                     /locus_tag="ACHOQY_003301"
     CDS             419..2656
                     /gene="nrfB"
                     /locus_tag="ACHOQY_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9679348.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2643..5615
                     /gene="nfrA"
                     /locus_tag="ACHOQY_003302"
     CDS             2643..5615
                     /gene="nfrA"
                     /locus_tag="ACHOQY_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9679349.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSRKAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5616..6506
                     /gene="ybcH"
                     /locus_tag="ACHOQY_003303"
     CDS             5616..6506
                     /gene="ybcH"
                     /locus_tag="ACHOQY_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9679350.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6689..7450
                     /gene="envY"
                     /locus_tag="ACHOQY_003304"
     CDS             6689..7450
                     /gene="envY"
                     /locus_tag="ACHOQY_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP9679351.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            7963..8916
                     /gene="ompT"
                     /locus_tag="ACHOQY_003305"
     CDS             7963..8916
                     /gene="ompT"
                     /locus_tag="ACHOQY_003305"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9679352.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9103..10587
                     /locus_tag="ACHOQY_003306"
     CDS             9103..10587
                     /locus_tag="ACHOQY_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9679353.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(10771..10983)
                     /locus_tag="ACHOQY_003307"
                     /pseudo
     CDS             complement(10771..10983)
                     /locus_tag="ACHOQY_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11133..11801
                     /locus_tag="ACHOQY_003308"
     CDS             11133..11801
                     /locus_tag="ACHOQY_003308"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9679354.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(11746..11883)
                     /locus_tag="ACHOQY_003309"
     CDS             complement(11746..11883)
                     /locus_tag="ACHOQY_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFP9679355.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(11856..12440)
                     /locus_tag="ACHOQY_003310"
     CDS             complement(11856..12440)
                     /locus_tag="ACHOQY_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9679356.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(12440..>15202)
                     /locus_tag="ACHOQY_003311"
     CDS             complement(12440..>15202)
                     /locus_tag="ACHOQY_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9679357.1"
                     /translation="FPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEALRRFELMV
                     EEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAGQAASSAQS
                     ASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSAATSASTAT
                     TKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     EATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEA
                     TRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLS
                     SATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAETP
                     KAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATV
                     TMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTN
                     FAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVY
                     SLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGW
                     TIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHS
                     LSGSTSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMANTNS
                     AGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFN
                     YIVRLA"
BASE COUNT         3867 a         3835 c         3678 g         3822 t
ORIGIN      
        1 gcagaattat tttcagggat acggcacatc gctattaata ttttaacgaa tgataaggta
       61 ttcaaggcag ggttaagacg taagatgcga aaagcagcca tggacagaaa ctatctggcg
      121 tcagtccttg cggggagcgg gctttcgtaa tcttgccctg cctgctcacc tcctccaggc
      181 acttccacta cattatgacc tgctcggtgc caccgctcct tttcactatg acgttaacct
      241 cccctccttg acaccatgct tttgtctaca ttctacgcaa acggcaactt ttataaccag
      301 tgtaaaaata acgtgccgca atagtcatac ctattaatgg taaaaagctg tcacaattca
      361 taaaaaacct taatatacgc caccctaaac ataaccagcg ttaatgtaag gtttttgtgt
      421 ggactggctt cttgatgttt ttgcaacctg gctctacggc ttaaaagtaa tcgcgataac
      481 gttagcggtc atcatgttca tcagcgggct ggacgatttt tttattgatg tcgtctactg
      541 ggtacgccgc attaaacgca agttgagtgt ttatcgccgc tacccgcgaa tgagttaccg
      601 cgaactgtat aaaccagatg aaaaaccgtt agcgattatg gttccggcgt ggaatgaaac
      661 gggcgtcatc ggcaatatgg ccgagctggc ggcgaccacg ctcgactacg aaaactatca
      721 tatctttgtt ggcacctacc ccaacgaccc cgatactcaa cgtgatgttg acgaagtgtg
      781 cgctcgcttt ccgaacgtgc ataaggtagt ctgcgcgcgt cctggtccca ccagcaaagc
      841 cgactgtctg aacaacgtgc tggacgccat cacccagttt gaacgtagcg ccaatttcgc
      901 ttttgctggt tttattctgc atgacgccga agatgtgatt tcaccgatgg aattgcgtct
      961 gttcaactat ctggtcgagc gtaaagatct gattcagatc ccggtgtatc cgttcgaacg
     1021 cgaatggacg cacttcacca gcatgactta cattgatgag ttttcagagc tgcatggcaa
     1081 agatgttccg gtgcgtgaag ccctcgccgg acaagtgccc agcgcaggcg tcggcacctg
     1141 tttcagccgc cgcgccgtga ccgctctgtt agctgacggt gacggtattg ctttcgacgt
     1201 gcagagtctg actgaagatt acgacattgg tttccgcctg aaagagaaag gtatgacgga
     1261 aatttttgtc cgttttccgg tggtggacga agccaaagaa cgcgagcagc gtaaattttt
     1321 acagcacgca cggacgtcaa acatgatctg cgtgcgcgaa tatttccccg ataccttttc
     1381 gactgcagtt cgacaaaaat ctcgctggat catcggcatt gttttccagg gctttaaaac
     1441 ccacaaatgg acctccagcc tgacgctgaa ctactttctc tggcgcgacc gcaaaggggc
     1501 aatcagtaac tttgtcagct tcctcgcgat gctggtgatg ctccagcttt tgctgttgct
     1561 ggcgtatgaa agtttgtggc ccgatgcctg gcatttcctt tctattttta gcggcagcgc
     1621 atggttaatg accctgctgt ggctaaactt tggcttgatg tttaaccgca tcgtgcagcg
     1681 ggtgattttc gtcactggct actacggtct gacgcagggg ctgctatctg tcctgcgtct
     1741 tttctggggc aacctgatta acttcatggc caactggcgc gcgctaaaac aggtacttca
     1801 acacggcgat ccacgtcgcg tcgcgtggga taaaacaacg catgacttcc ccagcgtgac
     1861 tggcgatacc cgctcgttgc gcccgttagg ccaaattctg ctggaaaatc aggtcatcac
     1921 tgaagaacaa ctcgatacag cactgcgtaa tcgcgtcgaa ggtctacgcc tgggcggttc
     1981 aatgctgatg caggggctga ttagcgccga gcagctggca caggcgctgg cagagcaaaa
     2041 cggcgtggcg tgggaatcca tcgatgcctg gcagatcccg tcctcgctga ttgccgaaat
     2101 gccagcctcc gtagcgctgc attatgcagt actgccgctg cgtctggaaa atgatgagtt
     2161 aattgtcggc agtgaagatg gtattgaccc tgtttcgctg gcggccctga cgcgtaaagt
     2221 cggacgcaaa gtgcgttacg tcattgttct gcggggacaa attgtcacgg ggttacgtca
     2281 ctggtatgca cgccgacgcg gtcacgatcc gcgggcaatg ttgtacaatg cggttcagca
     2341 tcagtggctc acggaacagc agaccggtga aatctggcgg caatatgtgc cgcatcagtt
     2401 cctgttcgcc gaaatactga ccacgctcgg tcatattaat cgttcagcaa ttaacgtgtt
     2461 gttattgcgc catgaacgca gttctctgcc gctcggcaag tttttggtca ccgaaggcgt
     2521 tatcagccag gaaacgttgg atcgcgtcct gacaattcaa cgcgaattac aagtttcgat
     2581 gcaatcacta ttactcaaag caggtttaaa cacagaacag gttgcgcaac tggagtccga
     2641 aaatgaagga gaataacctt aatcgcgtca tcggatggtc tggtttactg ctgacgtctt
     2701 tattgagtac cagcgcactc gcagacaata tcggcaccag cgcagaagag ctggggctga
     2761 gcgattatcg ccattttgtt atttatcccc gtctcgataa ggcgctgaag gcacagaaaa
     2821 ataacgacga agcaaccgcc atccgcgaat ttgaatatat acaccagcag gtgccggata
     2881 atattccgct gactttatac cttgcggaag cctatcgcca ttttggtcat gatgaccggg
     2941 cgcggctgtt gctggaggat caactgaaac gtcacccagg agatgcccga cttgagcgca
     3001 gtctggcggc tattccagtt gaagtgaaaa gcgttacgac tgttgaagaa ctgcttgccc
     3061 agcaaaaagc gtgcgatgct gcgccgaccc tgcgttgtcg cagtgaagtc gggcagaatg
     3121 ccctgcggct ggcacagtta cctgtcgcca gagcgcaact gaacgatgcg acgtttgctg
     3181 catcgccgga aggaaaaacg ctgcgaaccg atctgctgca acgggcaatc tacctgaaac
     3241 aatggtccca ggcagatacg ctatacaatg aagcacgcca gcagaacaca ttaagcgcgg
     3301 cagaacgccg tcagtggttt gacgtgcttc ttgccgggca gctggacgat cggatcctgg
     3361 cactgcaatc acaggggatc ttcaccgatc ctcagtcata tattacttac gcgaccgcgc
     3421 tggcttatcg tggcgaaaaa gcacgcctcc agcattatct cattgaaaat aagccgctgt
     3481 ttaccacgga cgcacaagag aaaagttggc tctatctgtt atctaaatac agcgccaacc
     3541 ccgttcaggc attggcgaat tatacggtgc agtttgccga taaccgccag tatgttgttg
     3601 gcgcgacgct accggtgctg ttaaaagaag gtcagtacga cgcagcgcaa aaactgctcg
     3661 ccaccctccc cgccaatgaa atgcttgagg agcgttatgc tgtcagcgtg gcgacccgta
     3721 acaaggctga atctctgcgt ctggcacgat tgctgtatca gcaagaaccg gcaaatctta
     3781 cccgcctaga tcaactgacc tggcaactga tgcagaacga gcagtcacgc aaagctgccg
     3841 atttattgct gcaacgctat cctttccagg gcgatgcgcg tgtcagccag actttaatgg
     3901 cgcgactggc gtctctgctg gaaagtcatc cttacctggc aactccagcg aaggtggcga
     3961 ttttatcaaa acccttgccg ctggcggcgc aacgtcagtg gcaaagccag ttgccgggta
     4021 ttgcagataa ttgcccggca atagttcgct tgctgggcga tatgtcgcct tcctacgatg
     4081 ccgccgcctg gaaccgtctg gcaaagtgtt atcgggacac gctacccggt gtggccttgt
     4141 atgcatggct tcaggccgaa caacgacaac cgaacgcctg gcaacatcgt gcggtagcct
     4201 atcaggcgta tcaggttgag gactacgcca ccgcactggc ggcctggcag aaaatcagtc
     4261 ttcacgacat gagcaatgag gatctgcttg ctgctgccaa taccgcccag gcggcaggaa
     4321 atggtgcagc tcgcgatcgc tggctacaac aggcagaaca acgtggactg ggaaacaatg
     4381 ccctctactg gtggctgcat gcgcaacgtt acattcctgg tcagccggaa ctcgcactga
     4441 gcgatctcac gcgctcaatc aatattgcgc cttctgccaa cgcttacgtt gcgcgggcga
     4501 caatttatcg ccaacgtcat aatgtcccgg cggcggtgag tgatttgcgc gccgcgctgg
     4561 aactggaacc gaataatagc aacatccagg cagcgcttgg ttacgccttg tgggatagcg
     4621 gtgatatcgc acagtcgcgg gaaatgctcg aacaggcgca taaagggctt ccggacgatc
     4681 cggcactgat ccgacaactg gcctacgtga accagcgtct ggatgacatg cctgcgacgc
     4741 agcactacgc ccggctggtg attgatgaca ttgataatca ggcgctgata accccactga
     4801 ccccagagca aaatcagcaa cgcttcaatt tccgccgttt gcatgaggag gtcggtcgcc
     4861 gctggacgtt cagtttcgat tcttctatcg gcttgcgttc cggggcaatg agcaccgcta
     4921 acaataatgt cggcggcgca gcgccaggga aaagctatcg tagctacggg caactggaag
     4981 ccgagtaccg catcggacgc aatatgctgc tggaaggcga cctgctctca gtttatagcc
     5041 gcgtctttgc cgataccgga gaaaacgggg tgatgatgcc ggtgaaaaat ccgatgtccg
     5101 gcaccggtct gcgctggaag ccgctgcgcg atcagatctt tttcctcgcc gtcgaacagc
     5161 agttgccgct gaacggccaa aatggcgcat ccgataccat gctgcgcgcc agcgcctcat
     5221 tctttaatgg cggcaaatac agcgacgaat ggcacccgaa cggttcaggc tggtttgccc
     5281 aaaacctgta cctcgatgcg gcgcaatata tccgccagga tattcaggcg tggacggcag
     5341 attatcgcgt cagctggcat cagaaggtag ctaacggaca gactattgag ccttacgctc
     5401 acgttcagga caacggctat cgtgataaag gcactcaggg cgcgcagctt ggcggagtcg
     5461 gggtccgctg gaatatctgg accggcgaga cgcactacga cgcctggccg cacaaagtca
     5521 gtctcggcgt cgagtatcaa cataccttta aggcgattaa tcaacgtaac ggagagcgca
     5581 acaacgcgtt tctcaccatt ggagtgcact ggtaaatgcg taagttcatt ttcgtattgc
     5641 tgacactgct tttggtcagc cctttttcct ttgcgatgaa aggtattatc tggcaaccac
     5701 aaaaccgaga tagtcaggtt accgataccc agtggcaggg gctgatgagt cagttacgtt
     5761 tgcaaggctt cgataccctg gttttgcaat ggacccgtta cggcgatgca ttcacccagc
     5821 cagaacagcg cgcgttattg tttaagcgtg ccgcagctgc gcaacaggct ggcctgaagc
     5881 tgattgtcgg gctgaacgcc gatccggaat tttttatgca ccagaaacag tcgtccgcag
     5941 cgctggaaag ctatcttaat cgcctgctgg ctgccgatct ccagcaagcc agattatgga
     6001 gcgccgcgcc tggcgtaacg ccggatggct ggtacatcag cgcggaaatt gacgacctga
     6061 actggcgcag cgaagctgcc cgtcagcctt tgctaacatg gttaaacaac gcgcagcggc
     6121 tgattagcga tgtttcagca aaaccggttt atatcagtag ttttttcgcc ggaaacatgt
     6181 cgcccgatgg ctatcgccaa ctgctggaac aagttaaagc aaccggcgtt aatgtctggg
     6241 tacaggatgg cagcggcgtg gataaactta ccgctgaaca gcgtgaacgt tatctacagg
     6301 ccagcgccga ttgccaaagt cccgcccctg ccagcggcat tgtttatgaa ctgtttgtcg
     6361 ccggcaaagg caaaaccttt acagcgaaac cgaaaccgga tgcagagatc gcctcgctgt
     6421 tagcgaaacg ttcctcttgc ggtaaagaca ctctctattt ctctctgcgc tatttgcccg
     6481 tcgcgcacgg cattctcgag tattaaatct cctccaggta agtcgggtac gacctggctt
     6541 acctctttcg ctctcgtcag aattccatca agattaattg ctaaaaagcg tgctaaagaa
     6601 agaagatcgt tatcggcgaa cgggccgatc actcatgcag gaagcataga tcctttgagc
     6661 ggagtttctg atatctgagg agtgcgaaat gcaattgagc agcagtgaac cttgcgtggt
     6721 gatcctgacc gaaaaagagg tagaggtaag cgtcaataac catgctacgt ttacccttcc
     6781 gaaaaactac ctggccgcct tcgcgtgcaa caataacgtc attgaactct caacgttaaa
     6841 tcacgtatta atcccccaca tcaaccgtaa catcatcaac gattatctgt tgtttttaaa
     6901 taagaactta acctgtgtaa agccctggtc gcggctggca accccggtta tcgcttgtca
     6961 tagccgtaca ccggaagtgt tccggctagc cgccaaccac agcaagcagc aacccagcaa
     7021 accctgcgaa gcggagttga cgcgcgcatt actttttacc gtattgtcta actttcttga
     7081 gcaatcgcgg tttatagccc tactgatgta tatcttacgc agcagcgtcc gcgacagcgt
     7141 ctgccgcatt attcaaagcg atattcagca ttactggaat ctgcgaattg tcgccagttc
     7201 gctatgttta agccccagcc tactcaaaaa gaaattaaaa aacgaaaata ccagctatag
     7261 ccagattgtc acagagtgtc gtatgcgtta cgccgtacag atgttattga tggataacaa
     7321 aaatatcact caggtggcgc aattatgtgg ctatagcagc acgtcgtact ttatctctgt
     7381 ttttaaggcg ttttacggcc tgacaccgtt gaattatctc gccaaacagc gacaaaaagt
     7441 gatgtggtga agggcaaagc ggaaacggat aagacgggca taaatgaaga agaaatggcg
     7501 cgccctgcag gattcgaacc tgcggcccac gacttagaag ttcctagaac gacattttaa
     7561 gtcaacaaat taccgcgcca tctctgcgct cacacgtccc actacctcaa aacatgtaaa
     7621 gccttgcaag ccattgtgag gccttatgtg tctcagtttt gtccctcttt tttgtactaa
     7681 aaaacatagc aattgaggat aaacctcatg ctattttcgc ttatatgcct ttaaaggcat
     7741 ggcgcttaaa tagataaaaa caccacaaaa gcataaaaaa ccacacagta aaaccgcaac
     7801 ataaaacaat aacagataat taaaccaaaa acagatagcg cattgtgata atcattcaat
     7861 actaaacaaa atataaacag tggagcaata tgtgattgac tcattaagtt agatataaaa
     7921 aatacatatt caatcattaa aacgattgaa tggagaactt ttatgcgggc gaaacttctg
     7981 ggaatagttc tgacaacccc tattgcgatc agctcttttg cttctaccga gactatatcg
     8041 tttactcctg acaacataaa tgcggacatt agtcttggaa ctctgagcgg aaaaacaaaa
     8101 gagcgcgttt atctagctga agaaggaggc cgaaaagtca gtcaactcga ctggaaattc
     8161 aataacgctg caattattaa aggtgcaatt aattgggatt tgatgcccca gatatctatt
     8221 ggggctgcgg gctggactac tctcggcagc cgaggtggca atatggtcga tcaggactgg
     8281 atggattcca gtaaccccgg aacctggaca gatgaaagta ggcaccctga tacacaactc
     8341 aattatgcca acgaatttga tctgaatatc aaaggctggc tcctcaacga acccaattac
     8401 cgcctgggac tcatggccgg atatcaggaa agccgttata gctttacagc cagaggtggt
     8461 tcctatatct acagttctga ggagggattc agagatgata tcggctcctt cccgaatgga
     8521 gaaagagcaa tcggctacaa acaacgtttt aaaatgccct acattggctt gactggaagt
     8581 tatcgttatg aagattttga gctaggtggc acatttaaat acagcggctg ggtggaagca
     8641 tctgataacg atgagcacta tgacccagga aaaagaatca cttatcgcag taaagtcaaa
     8701 gaccaaaatt actattctgt tgcagtcaat gcaggttatt acgtaacacc taacgcaaaa
     8761 gtttatgttg aaggcgcatg gaatcgggtt acgaataaaa aaggtaatac ttcactttat
     8821 gatcacaatg ataacacttc agactacagc aaaaatggtg caggcataga aaactataac
     8881 ttcatcacta ctgctggtct taagtacacc ttttaacgaa gttaaccaga ttttctcccc
     8941 ggtggtattg tataaccccg gggaatttgt ttacagttac aagtaggcaa tatccactaa
     9001 ttttgtaagc atttaaccta tgtcataacg tgtaatctct taccgcaaca caagacctca
     9061 atatattcaa atcttatctt tattccgata ttcagagaca caatgcgaaa aataattact
     9121 catttcaaag ttgttttaac gttacttcta ccagtaaccg tatctgccca gcagatacag
     9181 tggcaatcct gtatggccag tcaattcaac cactggtttg gtgaggaaaa accgtctcct
     9241 gacttactat gtggttattt gtctgttcca ttaaaatata cagacacagg cggagatgct
     9301 tcttatgaaa aaaaatcaca agtcaaacta gcgttgacaa aattgccggc aaaaagcaag
     9361 cataaaggaa gtatcctgat aataagtggt ggtcccgggt taccaggcat aaatccttat
     9421 attaactttg actggccagt cacaaatctt cgtgagtcat gggatattat tggatttgat
     9481 cctcgaggcg tcggacagtc cactccgaca ataaactgcc ggcaatcaga tacagagact
     9541 caggaaaaca taaccgaaaa gcaacaagta ttaaataaaa ttaatgcctg tatccataat
     9601 accggagccg aagtcattcg ccatatcggc tctaacgagg ctgtatacga tattgatcgt
     9661 attaggcaag ccttggggga taaacaactg acagccgtgg cgtattcgta tggaactcaa
     9721 attgcagcct tatatgcaga acgttttccc tacaacgtaa gatctatcgt tcttgatgga
     9781 gtcgtcgata tcgatgacct ggaggacaac ttcacatggc aactcaaaca ggcacagagt
     9841 tatcaggaaa cgtttgatcg ctttgcatcc tggtgtgcgc gtacaaaaag ttgcccgctt
     9901 tcttcagaca gagataaggc aataactcag ttccatgagc tattatcaaa attacatcac
     9961 aaacctttat tagacagtaa gagagaaaat atatcttcag atgaactcat atcattaaca
    10021 acagaccttc tgctatggcg ttcatcatgg ccaacccttg caactgccat acgccagttc
    10081 tctcagggga ttgtcagtaa tgaaattgaa actgcgctca gtgctccgat agcctcagaa
    10141 gagtcaagcg atgcttcggg ggtaatcctc tgtgtagatc agggggatga gcaattaaca
    10201 ccagaagagc gaaaatcccg aaaagacgct cttgcgaatg ccttcccggc tattaacttt
    10261 gacaatggac gttccgattc acctgatttt tgtgaattat ggccaataca tagcgacctg
    10321 aacaaaactc gcctgaaaaa tactgttctg ccctctggtt tactgtttat agcacacaaa
    10381 tacgacccaa caacgccctg gattaatgcc cgtaagatgg cagagaaatt ttccagcccg
    10441 ttactaacaa taaatggtga tgggcataca ttagctctca ccggagttaa tttatgtgta
    10501 gataaagcag ttgtacatca cctgatcact ccacaaaaaa tagaaaatat atactgccca
    10561 ggaaattctg aagcagaaat acaataattt caaaatattc cgctatttgc tcccaatgca
    10621 aaacatatat tgcattggga gcatattcat atttttgtta ttattgtatg aaatcgtctc
    10681 atgtacacgg tccttttttc gccgaaaact gatattactt taatcaaaag ttgacgtgcc
    10741 agattcgaag taaatttata aaatatcaaa tcaatataca ttttgcacct tcgagatagt
    10801 aatgactgtt actgtaacat gagttacaaa cagcttcata cccagagcta ctgatgtatc
    10861 aactcgattc aacaataccc gatatttatt ccatgcctcc agcaacgatc tttcttcctc
    10921 cgttgcgatt tccagatcta cagcatcctg cagtggcgca atatactcac tgaattcctg
    10981 gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgttgta tcgaagcata
    11041 ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca
    11101 taattgattt aattcacaaa taaaactata acatggtgaa attaatgaaa aaaaacacag
    11161 atgatggggc taaaatttac acaccactta ccctaaagct ttatgactgg tgggttttgg
    11221 gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt ccacactttc
    11281 tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt taccttactc
    11341 acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc ctgaacgcgg
    11401 catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat gtttttgaac
    11461 cttatcccgc ggcgttacat ggtcaatttg attccatttc catgttttac cttcttcact
    11521 gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg caggccttaa
    11581 ctgacgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg cacaatagct
    11641 tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac acaaaagatt
    11701 ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa accaaggttc
    11761 aaggtactgt agtaatgttt tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt
    11821 cttgcttaag acgtgctgcg gcataatccc aatgattact ccctgacagg gttcgtaggc
    11881 cactcaatat caggtgcagt tgatgtatca acacggttca gcaacacccg atactttttc
    11941 caggcttcca gcaatgaggt ttcttcctcc gttgcgattt ccagatctac agcatcctga
    12001 agtggcgcaa tatgctcact ggctacctgc atcaggctgt tttttatttc ttccgcctct
    12061 cggatccgga acagtttttc tgcttctgca tctttcaccc aggctgtgcc gttccacttc
    12121 tgaaactccc cttccggcga taaccaggta acattttccg gtaacggacc gagttcagaa
    12181 ataaataacg cgtcccctga cgctacgtca taaaccgttt taccccgatg gtcttcaacg
    12241 agatgccacg atgcctcatc actgttgaaa acagccacaa agccagcagg aatatctggt
    12301 ggtgcaatat cggtactgtt tgctggcaga cctgtatgag gcggaatata tgcatcacct
    12361 tcaccaataa attcattagt tccggccagc agattataaa tttttatggt ccgtgcttgt
    12421 tcactcattc tgaatgccat tatgcaagcc tcacaatata gttaaatgcg atgtttttga
    12481 cggtgttttc cgcgttacca gcagcgttaa cggtgatggt gtgtccatgt gagccaatcg
    12541 caacggagtg cgtatgagca ccaataccga cagtatgtgc gtgtgcacct gcgcttgcag
    12601 cagtgccgga cagtgagtgg gtatgcgcgc cagcagagtt cgtattcgcc atcgtgccaa
    12661 tgcccagacc aaccgcccct tgtacctggt atgagttact tccgacagct gtagaaccat
    12721 actgataggt gtctttaaaa gtgcttgtat taaacctgcg accatcaacg tgcgagtgtg
    12781 ctccggccga gcttgtagag ccgctcagac tgtgcgtatg agccccggtg ttattcgtgg
    12841 atttagtgcc gtaatcaaac gacgatgtgg ttttcgtccc caaatccgta ctggatgcgc
    12901 tggcgctgtg ggtgtgcgat ttaatgccgt cctgttcctg agacaatacg gcacgaccac
    12961 tggcgggttt gcccttaatc gtccagccac gcatatcagg gatcacgcct gacggataag
    13021 cggctgcaag tttcgggtat gcagatttgt caaaagtctg cccctgcatc agggcatagc
    13081 cagacggaac ggtatctgat ggccacggga ttggtgcgcc aggcggataa aactgctctg
    13141 atggcgtata gagtgaataa actgtaccgt ccgttaaccc ttccggctta ttagcagaat
    13201 atgctggtga cgtatgaatc gtcacgctgg cattactggt ataatcccat tgaatattta
    13261 caccagtcgc ataatttccg attgcaacgt aaatatcgta agtatcacca gatgtattga
    13321 cccaggcaaa atttgtaaac cctgtcgatg tgcgctgcca taaagcacca gtaatcccct
    13381 tcggattacc attacctgca cgcaaaacaa gttcagatat acctgcctgt tgaggtgacc
    13441 ccacgttaaa tcccgcgcca ccaatcaacg taattgaaac aacagaactc gcctgtggca
    13501 tggttaccgt tgctaatttg aaccaaccag caccaccgct gaatgacatt gttgttgagt
    13561 taagcgtacc aatatctttc ggcgtcagtg ttatatccgc tgaaagcgcc ttaccattca
    13621 ccttacgggc agaaggtacc cgaccattcg cattatcatt agctgctttc actgctttcg
    13681 gtgtctcggc aagcgtttca gatgcgctgt tggttgcact actgagctgg acaattcctt
    13741 tttgtgctgt cgtagcgtcc tgagcggtat atttcccgtt agcaaggtca taggctgcct
    13801 ttaccgactt tggcgttgcc gccagcattt cagatgtgct gttggtcgcg ctgctgagct
    13861 gaacgatccc tttctgtgct gtcgttgcat cctgtgcggt gtatttcccg ttagccaggt
    13921 catacgcggc cttaacggct tttggcgttg ccgccagtga ctcggaagtg ctgttagtcg
    13981 cactgctgag ctgtactatc ccctttttcg tcgtgctcgc atcctcaagc gccacggcgg
    14041 atgcaatatc ctctgcccgt ttagctgctg tctcggcgcg cgttgccgcg gattccgccg
    14101 tacttttgct ctgagctgcc gccgtcgcac tgccagctgc ctctgtcgcc ttcgtggatg
    14161 ccgtcgtggc gctgctcttc gctgctgacg cctgtctggt cgcctcatct tttgaagcag
    14221 acgccgatga tgccgatgac gccgccgaac tggctgacga tgcggcagcc gtttttgagg
    14281 attctgcgct ggtttccgac gctttcgcgt tcgtttcgga tgtcttcgct gcggaagcag
    14341 aactcgctgc tgcgctggcc tgttcagtgg cttcgccagc cttcgttgtg gctgttgaag
    14401 cggatgatgc ggcgctttct gccgattttc cggcggcggt ggcactggct gaggcctgcc
    14461 cggcacttgt tgacgcggca ctggcagacg acgcagccgc tgtttttgag cctgccgcag
    14521 ctgaggcgct ctgtcccgct gccgtctcag aagacttagc gttcgtctcg gacgtttttg
    14581 ccgccttcgc ggaatttcct gccgccgttg ccgaggaagc ggcactactg gcgcttgatg
    14641 atgcgttcgt ttctgatgat tttgccgctt cttttgaagc cgacgcatcc cgggctgagg
    14701 tggcagcttc tgacgctttc gtggtcgcgg tggatgcaga agtggctgct gattgttgtg
    14761 acgctgccgc attcgtttct gacgttttcg ccgcagcggc actggtggcc gccgcgcttt
    14821 ttgaggactc tgcagcggca gcactttttg atgcttcagt ggcctttgtt gatgccgttc
    14881 ctgcgctgga agacgctgac tgagccgacg acgcggcctg tccggctgac gtgctggctg
    14941 cgcgtgctga gcctgcagca tcagtcgcat gggttgccgc ctcacgggct gatgtgctgg
    15001 catcgctggc tgacttcttc gcggctgccg tgttctgtgc caccgcggac gcgttacgcg
    15061 ccacctcttc caccatcagt tcaaaacggc gcagtgcctc aggacgggca tcatcctccg
    15121 tcatggcacc gagaaaatca ttcagcgtac cgggtcggga atcttcatac acggtgatgg
    15181 tcccggcatg tgacggcggg aa
//
LOCUS       DAZKGS010000108        15156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKGS010000108 DAZKGS010000000
VERSION     DAZKGS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..1222
                     /gene="citC"
                     /locus_tag="ACHOQY_003312"
     CDS             164..1222
                     /gene="citC"
                     /locus_tag="ACHOQY_003312"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9679358.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1237..1533
                     /gene="citD"
                     /locus_tag="ACHOQY_003313"
     CDS             1237..1533
                     /gene="citD"
                     /locus_tag="ACHOQY_003313"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9679359.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1530..2438
                     /gene="citE"
                     /locus_tag="ACHOQY_003314"
     CDS             1530..2438
                     /gene="citE"
                     /locus_tag="ACHOQY_003314"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9679360.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2449..3981
                     /gene="citF"
                     /locus_tag="ACHOQY_003315"
     CDS             2449..3981
                     /gene="citF"
                     /locus_tag="ACHOQY_003315"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9679361.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3985..4536
                     /gene="citX"
                     /locus_tag="ACHOQY_003316"
     CDS             3985..4536
                     /gene="citX"
                     /locus_tag="ACHOQY_003316"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9679362.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4511..5389
                     /gene="citG"
                     /locus_tag="ACHOQY_003317"
     CDS             4511..5389
                     /gene="citG"
                     /locus_tag="ACHOQY_003317"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9679363.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5440..6903
                     /gene="citT"
                     /locus_tag="ACHOQY_003318"
     CDS             5440..6903
                     /gene="citT"
                     /locus_tag="ACHOQY_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9679364.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7017..7823
                     /gene="rna"
                     /locus_tag="ACHOQY_003319"
     CDS             7017..7823
                     /gene="rna"
                     /locus_tag="ACHOQY_003319"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9679365.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8053..8463
                     /gene="rnk"
                     /locus_tag="ACHOQY_003320"
     CDS             8053..8463
                     /gene="rnk"
                     /locus_tag="ACHOQY_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9679366.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8601..8690
                     /gene="yldA"
                     /locus_tag="ACHOQY_003321"
     CDS             8601..8690
                     /gene="yldA"
                     /locus_tag="ACHOQY_003321"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP9679367.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8694..9932)
                     /gene="ybdR"
                     /locus_tag="ACHOQY_003322"
     CDS             complement(8694..9932)
                     /gene="ybdR"
                     /locus_tag="ACHOQY_003322"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP9679368.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10153..10581
                     /gene="uspG"
                     /locus_tag="ACHOQY_003323"
     CDS             10153..10581
                     /gene="uspG"
                     /locus_tag="ACHOQY_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9679369.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="ACHOQY_003324"
     CDS             complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="ACHOQY_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9679370.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12438..13001)
                     /gene="ahpC"
                     /locus_tag="ACHOQY_003325"
     CDS             complement(12438..13001)
                     /gene="ahpC"
                     /locus_tag="ACHOQY_003325"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9679371.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13373..14119
                     /gene="dsbG"
                     /locus_tag="ACHOQY_003326"
     CDS             13373..14119
                     /gene="dsbG"
                     /locus_tag="ACHOQY_003326"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9679372.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14328..>15156
                     /gene="ybdO"
                     /locus_tag="ACHOQY_003327"
     CDS             14328..>15156
                     /gene="ybdO"
                     /locus_tag="ACHOQY_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9679373.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     "
BASE COUNT         3692 a         3955 c         3743 g         3766 t
ORIGIN      
        1 ttcacgccga aatactcctt aggatgtata gcgaaaggag aaaaagatat acctcgatca
       61 cccccctttc tcccaagtga aaataaaagg ttatcagttt gcaacattga acaacattcg
      121 ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat
      181 tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca
      241 tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga
      301 aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga
      361 atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta
      421 tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg
      481 ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg atggtgctga tggaaaacag
      541 cgccacgcgg ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa
      601 gattggctgc attgtgatga acgccaatcc cttcacgaat ggtcaccgtt atctgattca
      661 acaagctgcg gcacagtgcg actggttgca tctgttttta gtcaaagaag attcttcacg
      721 cttcccctat gaagaccggt tggatctggt gttaaaaggc accgccgata ttccacgcct
      781 gactgtgcat cgcggctccg aatacatcat ctcccgcgct acgttccctt gctacttcat
      841 taaagaacag agcgtcatta accattgtta caccgaaatt gatctgaaga ttttccgtca
      901 gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg
      961 cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc
     1021 accgcccatc gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc
     1081 ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc
     1141 tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc
     1201 tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt
     1261 gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat
     1321 atcgacctgc aaatcaatag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc
     1381 attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa
     1441 ggcgcactgg actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt
     1501 atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa
     1561 ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact
     1621 ccttcatcta cccggctgat gccctgatgt ttgacctcga agactccgtg gcattacgtg
     1681 aaaaagacac cgcccgccgc atggtttatc acgcgctgca acatccgctg tatcgcgata
     1741 ttgaaaccat tgtgcgtgtc aacgcgctgg attccgaatg gggtgttaac gacctggaag
     1801 ccgtcgttcg cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg
     1861 ttctggatat tgaaaaagag atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca
     1921 gcaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg
     1981 ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc
     2041 tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgcacgctgt tccattttgc
     2101 aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg
     2161 aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc
     2221 tgatcaaccc gcgtcagatt gatctgttgc acaacctcta cgcaccaacc cagaaagaag
     2281 tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg
     2341 tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc
     2401 tctctcgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa
     2461 attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat
     2521 cttgccgctt tccagaactc gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc
     2581 aaactgtgcg ccaacctgga agaagcgatt cgtcgctctg gtttacagga cggcatgacg
     2641 gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc
     2701 atcgcgaaga tgggctttaa aaacctgacc ctggcgtcca gctccctgag tgattgccat
     2761 gcgccgttgg tagaacacat tcgccagggc gtggttaccc gcatttatac ctccggcctg
     2821 cgcggtccac tggcggaaga gatctcccgt ggtctgctgg cagaaccggt gcaaatccac
     2881 tctcacggcg gtcgcgtgca tctggtacag agcggcgaac tgaatatcga cgtggctttc
     2941 ctcggcgtcc cgtcctgcga tgaattcggt aatgccaacg gctacaccgg taaagcctgc
     3001 tgcggctccc tcggctatgc aatggttgat gccgacaacg caaagcaggt cgtgatgctt
     3061 accgaagaac tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat
     3121 ttgatcgtca aagttgaccg cgttggcgat gctgcaaaaa tcggcgctgg cgcgacccgt
     3181 atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgtcaac
     3241 tcaggctact tcaaagaagg tttctccatg caaaccggca ccggcggcgc atcgctggcg
     3301 gtaacccgtt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctt
     3361 ggcggtatta ccgcgacgat ggttgacctg cacgaaaaag gtctgatccg caaactgctg
     3421 gatgtgcaga gctttgacag ccatgctgcg caatcgctgg cccgtaaccc caatcacatc
     3481 gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc
     3541 gacgtggtgg tactgagcgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc
     3601 ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcctct
     3661 gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta ttccgactct ggtggataac
     3721 gtgctgacct gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggtatc
     3781 gcagttaacc cggcacgtcc ggaactggca gaacgtctgc aggaagcggg cattaaagtg
     3841 gtttccattg agtggctgcg cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt
     3901 gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt
     3961 gtgcatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg
     4021 gtgtcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgtctgg
     4081 ctcaagcgcc atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa
     4141 gacagcgagg tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc
     4201 gcaaaacagg gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag
     4261 ggcatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa
     4321 catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgaaatt
     4381 ctctcccgcc gcgactattc actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca
     4441 gccgtctgcg cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggca
     4501 ctgctgaacg atgtcgatgc ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt
     4561 aatcgatgag tacgccctgc tgggctggcg cgccatgctg actgaagtca atctgtcacc
     4621 gaaaccaggc ctcgtggatc gcattaactg cggtgcgcac aaagatatgg cgctggaaga
     4681 tttccaccgc agcgcgctgg cgattcaggg ctggttaccc cgtttcattg aatttggtgc
     4741 ctgtagtgca gaaatggcac cggaagcggt actccacgga ttacgcccaa ttggtatggc
     4801 ttgcgaaggt gatatgttcc gcgccactgc gggcgtaaac acgcataaag gcagcatttt
     4861 ttctttaggg ctgctatgtg cggcaattgg ccgtttgctt cagctcaacc agccggtaac
     4921 gccaacaacc gtttgttcta cggcggcaag tttctgccgt ggtctgaccg atcgcgaact
     4981 gcgtaccaat aattcacaac tgacggcagg tcaacggttg taccagcagc ttggccttac
     5041 cggcgcacgc ggtgaagccg aagcgggtta tccactggtg atcaaccacg ccttgccgca
     5101 ttacctcact ctgctggatc aggggttaga tcctgaactg gcattgctcg ataccttgct
     5161 cctactgatg gcgatcaacg gcgataccaa cgttgcttcg cgcggtggcg aggggggcct
     5221 gcgctggcta cagcgcgagg cgcaaacatt attgcaaaaa gggggcattc gaacccccgc
     5281 cgatctcgat tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg
     5341 cggcagtgcc gacctgctga tccttacctg gtttttagca cagatttaat tatttaagca
     5401 cttgataaat ttgaaaatat taattttcgg agaacccgta tgtctttagc aaaagataat
     5461 atatggaaac tattggcccc actggtggtg atgggtgtca tgtttcttat ccctgtcccc
     5521 gacggtatgc caccacaggc atggcattac ttcgctgtgt ttgtggcaat gattgtcggc
     5581 atgatcctcg agccaattcc ggcaacagcg atcagtttta ttgcggttac tatttgcgtt
     5641 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
     5701 caaaaacagg cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta
     5761 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc
     5821 cttttcctgg tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt
     5881 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
     5941 tttccggtca ttaaaaacct gccgccgttg tttaaatcat tccccaacga tccgtccgcg
     6001 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
     6061 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
     6121 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
     6181 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
     6241 gaagtggcaa cctgggcggg tgatgaactg aaaaccatgg gtgcgctgac acgcagagag
     6301 tggacactga ttggccttgt attgctcagc ttaggtttgt gggtttttgg cagcgaagtc
     6361 attaatgcta ctgcagttgg tctgctggca gtttcgctaa tgctggccct gcacgttgtg
     6421 ccttggaaag atattacccg ctataacagc gcatggaaca cgctggtcaa cctggcaact
     6481 ctggttgtga tggctaacgg cctgactcgt tctggcttta ttgactggtt cgccggtacc
     6541 atgagtacgc acctggaagg attctcacca aacgcaacgg tgattgtact ggttctggtg
     6601 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     6661 gttattctgg ccgtcggtaa aggtattccg ggcgtaccaa tggaacaact gtgtatcctg
     6721 ctggtgctgt ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg
     6781 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     6841 ggggtgattt acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac
     6901 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg
     6961 gcttttttat gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga
     7021 aagcattctg gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     7081 acgccttagc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     7141 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     7201 gtcgcctgca aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc
     7261 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     7321 gttgcgctac tcgcccaatc ccgaatctac cagaagcgca cgccagccgg atgtgttcat
     7381 cgccggaaac cggattatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag
     7441 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     7501 ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg
     7561 aagccggaaa attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg
     7621 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     7681 gtaaccctgc gtatttgact gaaattcaga tctcgatcaa agctgacgca atcaacgctc
     7741 cgctttctgc aaactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg
     7801 tgattgataa agtgggttat taatcatctg atttacaaaa cacgctaatt cgtcttccat
     7861 aaaccgtgac gattaagaat tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7921 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7981 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     8041 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8101 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8161 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8221 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8281 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8341 gcactgctgg ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca
     8401 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt
     8461 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8521 acgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8581 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8641 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt
     8701 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8761 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca
     8821 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8881 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8941 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     9001 gcgtagacgc ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactggcgc
     9061 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
     9121 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
     9181 tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg gatcgcgccg
     9241 tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
     9301 gcaccgagca accgtgcaca ggcgattgtc aacaatccca ctggaccggc accatagact
     9361 gcaacgcttg aaccttgttg gatctgcgca ttttttgctg cctgccatgc cgttggcaga
     9421 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
     9481 acattccctt tagggacgcg gacatattcc gcctgtccac caggaacgcc accatacagg
     9541 tgactataac taaacaatgc cgcgggagct ggtatctgtt ttttattgag cgcagcgcct
     9601 ttacccgcat tggtattttc gcaggcggca tattgctgca ttcgacagaa aaaacagtcg
     9661 ccacaagcaa tgacaaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc
     9721 tttccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
     9781 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt tgccgtaata
     9841 cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
     9901 tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt tcaaaaaaga
     9961 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag gttttgggag
    10021 aattcctggt gttcagtgag aatatattaa tcacagaaac cgttcgtaag gaaaaagaac
    10081 cagacaaata tcagggataa atattataat catggcgaat gatttgttca tgattaacag
    10141 ggagaaaggg ttatgtataa gacaatcatt atgccagttg atgtatttga aatggaattg
    10201 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
    10261 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
    10321 cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac
    10381 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
    10441 gtcaatgagt tggcagaaga actgggggct gatgttgtag ttattggttc tcgcaaccca
    10501 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca tgccaatctg
    10561 ccggtgctgg ttgtgcgtta attgccagaa taaatatccc gccctgcccg gagccatccc
    10621 ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
    10681 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
    10741 cagagaggct ttggcacctt cgcctgtggc gatgataatc tgcttgtacg gaaccgtcgt
    10801 acagtcaccc gctgcgaaca cgcctttgac gttggtttcg catttcgcat caatgataat
    10861 ctcacccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc
    10921 aatctggacg aaaataccgg ccagttcgat gttgtgaata tcgccgctga cacgatctcg
    10981 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt gcgcattcag
    11041 aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
    11101 ttctggcgca aattcgagca gcgttacgtg ctcaacgata cccgccaggt caattgccgc
    11161 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcgggcc
    11221 gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc caggaacgtt
    11281 catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca gtaccgcgcc
    11341 agaagctgtt tcaatctgat gcagaccacc ttcaactgct gctgggatca gtttgctggc
    11401 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
    11461 cagcttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
    11521 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
    11581 aattgctgca gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg
    11641 cttgttcagc tcttctgccg cacgtttttc cgcgcccgta tcaattttgg caacgatttc
    11701 agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc
    11761 catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
    11821 gatgcgcggg ttcagtacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg
    11881 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
    11941 cagcagagac tgcgcttctt tcgacggatg accaccggtc catagcaacg ccagtaccag
    12001 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt tggaacctgg
    12061 gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt taaaggtgac
    12121 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
    12181 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
    12241 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
    12301 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
    12361 gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt tcgcgccgga gttggtagcg
    12421 cgaaagacga aggaaattta gattttacca accaggtcca gagacggagc cagagttgct
    12481 tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac gtactgtgct
    12541 gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg
    12601 attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc cagaccttca
    12661 tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc gatcatcgca
    12721 tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt gaagtgagta
    12781 tcggtagata ctgcgtatac gtctacgccc agtttctgca gttcttcgta gtggtcagca
    12841 acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa gaagaagacg
    12901 ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt tttgaatgcc
    12961 tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc ctccgtgttt
    13021 tcgatgagat gtaaggtaac caatttttgc cgattcggct aatgcgtttc cattatcaaa
    13081 tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc accgccagcg
    13141 gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac taccctttgg
    13201 tatcttcagt gcctgcctgc aggctgaggt gttgccctta caaatgcaac aacgacatgg
    13261 attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac ctatgacagt
    13321 gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat taatgttaaa
    13381 aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac ttcctgctcc
    13441 agtaaaagcg attgaaaaac agggcattac aatcatcaaa acattcgatg cccccggagg
    13501 aatgaaaggt tatctcggaa agtatcagga tatgggcgtc accatctacc tgactccaga
    13561 tggtaagcac gctatctctg gttacatgta caacgagaaa ggtgaaaacc tgagtaacac
    13621 acttatcgaa aaagaaattt acgcaccagc cggacgcgaa atgtggcaac ggatggaaca
    13681 atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg tgttcgccga
    13741 tccgttctgc ccatattgta aacagttctg gcagcaggcg cgcccgtggg tagattctgg
    13801 caaagtgcaa ttaagaacat tgttggttgg ggttatcaag ccagaaagcc cggcgacagc
    13861 agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caagaatatg aatcctctgg
    13921 tggcaagctt aagctaaacg tgcctgcaaa cgtaagtaca gaacaaatga aagtgttaag
    13981 cgccaacgag aaactcatgg acgatctggg ggcaaatgtc acaccagcga tctattacat
    14041 gagtaaggaa aatacgctgc aacaggccgt ggggttgccc gatcagaaaa cgcttaatat
    14101 cattatgggg aataaataaa agataattca tggatggttg aaatacatcc ctgcttttca
    14161 accatcctaa aaaagcatga attgctcgct ctgccctaag tggctatgct attttagaaa
    14221 aacttaatcc tgagttaagc aaaaaaatat aatcaataaa aatatatacg caaaaaacat
    14281 tgattaagtg aatataccat ggaagaaaaa tataaccgga gtagtgtatg gccaatctct
    14341 acgacttgaa aaagttcgac ttaaaccttc ttgtcatatt tgagtgtatt taccagcatt
    14401 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
    14461 tacaacgctt acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg
    14521 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
    14581 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atttacggcc
    14641 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
    14701 ctgtcgagat tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg
    14761 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
    14821 gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta
    14881 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat
    14941 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
    15001 catttcgtgg ttcctctttg ctgacaatca ttaatagcat tgcagttaca gatttactgg
    15061 gaatagtccc ctatgagctt tataattcct accgtgactt tcttaattta aaagagataa
    15121 aactagaaca tccgttacct tcaataaagc tttaca
//
LOCUS       DAZKGS010000109        15044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKGS010000109 DAZKGS010000000
VERSION     DAZKGS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1368
                     /gene="hpaG"
                     /locus_tag="ACHOQY_003328"
     CDS             79..1368
                     /gene="hpaG"
                     /locus_tag="ACHOQY_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFP9679374.1"
                     /translation="MKGTIFVVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1365..2831
                     /gene="hpaE"
                     /locus_tag="ACHOQY_003329"
     CDS             1365..2831
                     /gene="hpaE"
                     /locus_tag="ACHOQY_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9679375.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2833..3684
                     /gene="hpaD"
                     /locus_tag="ACHOQY_003330"
     CDS             2833..3684
                     /gene="hpaD"
                     /locus_tag="ACHOQY_003330"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFP9679376.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3694..4074
                     /locus_tag="ACHOQY_003331"
     CDS             3694..4074
                     /locus_tag="ACHOQY_003331"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFP9679377.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4185..4988
                     /gene="hpaH"
                     /locus_tag="ACHOQY_003332"
     CDS             4185..4988
                     /gene="hpaH"
                     /locus_tag="ACHOQY_003332"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFP9679378.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4999..5787
                     /gene="hpaI"
                     /locus_tag="ACHOQY_003333"
     CDS             4999..5787
                     /gene="hpaI"
                     /locus_tag="ACHOQY_003333"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFP9679379.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5962..7338
                     /gene="hpaX"
                     /locus_tag="ACHOQY_003334"
     CDS             5962..7338
                     /gene="hpaX"
                     /locus_tag="ACHOQY_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFP9679380.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7348..8238
                     /gene="hpaA"
                     /locus_tag="ACHOQY_003335"
     CDS             7348..8238
                     /gene="hpaA"
                     /locus_tag="ACHOQY_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFP9679381.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8489..10051
                     /gene="hpaB"
                     /locus_tag="ACHOQY_003336"
     CDS             8489..10051
                     /gene="hpaB"
                     /locus_tag="ACHOQY_003336"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFP9679382.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10069..10581
                     /locus_tag="ACHOQY_003337"
     CDS             10069..10581
                     /locus_tag="ACHOQY_003337"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFP9679383.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10974..13124
                     /gene="btsT"
                     /locus_tag="ACHOQY_003338"
     CDS             10974..13124
                     /gene="btsT"
                     /locus_tag="ACHOQY_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9679384.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13255..13458
                     /locus_tag="ACHOQY_003339"
     CDS             13255..13458
                     /locus_tag="ACHOQY_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9679385.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13469..14425
                     /gene="yjiA"
                     /locus_tag="ACHOQY_003340"
     CDS             13469..14425
                     /gene="yjiA"
                     /locus_tag="ACHOQY_003340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9679386.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14512..14703)
                     /locus_tag="ACHOQY_003341"
     CDS             complement(14512..14703)
                     /locus_tag="ACHOQY_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9679387.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14743..>15044)
                     /locus_tag="ACHOQY_003342"
     CDS             complement(14743..>15044)
                     /locus_tag="ACHOQY_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane domain protein"
                     /protein_id="HFP9679388.1"
                     /translation="IVSTILVAFASKNVEGISYYVLKSTIDFEWRNQVKCGELNISRP
                     DERYFGFNTDKYTVFYSNREGKWGVNELKCKKGSDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         3504 a         4117 c         4097 g         3326 t
ORIGIN      
        1 ttgccaaact acgcaatcgg gaataaaagt aatcattaac atattaatga ttaagatgat
       61 cgaatccgag gagtggtaat gaaaggcact atcttcgtcg tagcgttgaa ccatcgcagc
      121 caacttgatg catggcagga agcgttccag caatccccct acaaagcccc gcctaaaact
      181 gcggtctggt ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt
      241 ccacagggtg aaaaggtact gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg
      301 acgaaagtac gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac
      361 gtcagcctgc cggaagagag cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga
      421 ttctgcccca ttggcgaaac cgtggctctc agcaatgtcg ataatctgac catctatacc
      481 gagatcaacg ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgcg
      541 cagttgctga gcgccctgag cgaatttgcc acactgaatc caggcgatgc cattctgctc
      601 ggcacgccac aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa
      661 ggtttcccgc cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcgcaagagc
      721 ttcccaacgc agccacaccc gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac
      781 cacgccagcg aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca
      841 aataccctca ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac
      901 tatgaagcgg agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat
      961 gccatggatt atgtcgcggg ctacaccgtg tgtaacgact acgccattcg cgactatctg
     1021 gaaaactact accgccctaa cctgcgggta aaaagccgcg acggactgac gccgatgctt
     1081 tcaaccatcg tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc
     1141 gtcaacggcg agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc
     1201 ctgatcgcct acttaagcga atttatgacc ctgaatccgg gcgacatgat cgccaccggc
     1261 acaccaaaag gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc
     1321 gtgggctgcc tggtgaaccg aattgtgagt gaggaaacag cgaaatgaaa aaagtaaatc
     1381 attggatcaa cggcaaaaat gttgcaggta acgactactt ccagaccacc aatccggcaa
     1441 cgggtgaagt gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag
     1501 cggcagcgaa agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc
     1561 tgatgcgccg tctgggcgat ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa
     1621 ccgcggacac cggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttccc
     1681 acaactttga atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg
     1741 acgacaagat gctcaactac acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc
     1801 cgtggaacgt accgtttatg accgccacat ggaaggtcgc gccgtgtctg gcgctgggca
     1861 atactgcggt gctgaagatg tcagaactct ccccgctgac cgctgaccgc ctgggtgagc
     1921 tggcgctgga agccggtatt ccggcgggcg tgctgaacgt ggtacagggc tacggcgcaa
     1981 ccgcagggga tgcgctggtc cgtcatcatg acgtgcgtgc cgtgtcgttc accggcggta
     2041 ccgccaccgg gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg
     2101 gcggaaaatc gccagtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg
     2161 ccctgttcac catcttctcg atcaacggcg agcgctgcac cgccggttcg cgcatcttta
     2221 ttcagcaaag catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc
     2281 gcgtgggcga tccgaacgat ccgaataccc aggttggggc acttatcagc cagcaacact
     2341 gggaaaaagt ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg
     2401 gcggcccgga taaaccgtct gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc
     2461 caacggtgct ggcagacgta gataaccgta tgcgcgtcgc ccaggaagag attttcgggc
     2521 cggtcgcctg cctgctgccg tttaaagacg aagccgaagg cttacgcctg gcaaacgacg
     2581 tggagtacgg cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg
     2641 cgcgtggcat tgaagcaggc atggtgttcg tcaataccca gaacgtccgc gacctgcgcc
     2701 agccgtttgg cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg
     2761 aagtgttcgc ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat
     2821 ggggagtctg atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg
     2881 tatctctctg aactaccagg aaaaaaccac ggttgtcgcc agggtgcgat cgacgggcat
     2941 aaagagatca gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgacacc
     3001 cactggctgg tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac
     3061 accagtaacg aactgccgca ttttattcgt gacatgacct acaactacga gggcaacccg
     3121 gagttggggc agctaattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac
     3181 aatattccca gcctgaaact ggaatacggc acgctggtgc cgatgcgcta catgaatgaa
     3241 gacaagcact tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac
     3301 agccgcaagc tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg
     3361 gtccttgcca gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg
     3421 atgaacagct acacccgcga gttcgaccgc cagatggacg agcgcgtggt gaagctgtgg
     3481 cgcgaaggcc agttcaaaga gttttgcaat atgctgccgg agtacgccga ctactgctac
     3541 ggcgaaggca atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac
     3601 gacggcaagg tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac
     3661 gctgttttcc cgcttcccgc gtaaggacgt tttatgccgc actttatcgt tgaatgcagt
     3721 gataacatcc gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg
     3781 gcagccacgg gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc
     3841 tggcagatgg ccgacgggca gcatgattat gccttcgtgc atatgacgtt gaaaatcggc
     3901 gcaggtcgca gcctggaaag ccgccagcag gcgggtgaaa tgctgtttga actgattaaa
     3961 acgcacttcg ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag
     4021 ctgcatccga cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca
     4081 gattgcccgg tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc
     4141 cggataaggc gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacaca
     4201 cccacaccct gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg
     4261 cgatctcgct ggattacccg gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat
     4321 gggttcgcct gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct
     4381 cgaaagcgat gcaggcaagc tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg
     4441 acatgttctt ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg
     4501 aagtggagct ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg
     4561 acgtctacaa cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc
     4621 acaacatcga tccggaaacc cagcgcccgc gcaaagtttt cgacaccatt tctgataacg
     4681 ccgccaacgc cggggtgatc ctcggaggtc gtccgatcaa accagacgaa ctggatctgc
     4741 gttggatctc cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg
     4801 gcgtgctgaa tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg
     4861 acgtacaact ggaagccggg caaatcattc tcggtggttc gttcacccgc ccggttccgg
     4921 cgcgtaaggg cgacaccttc cacgtcgatt acggcaacat gggttccatt agctgccgct
     4981 ttgtttaagg agagaacgat ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg
     5041 cagatcggat tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca
     5101 ggattcgact ggttgttgat cgacggtgag cacgcgccga ataacgtgca aaccgtgctc
     5161 actcagctac aggcgattgc gccctacccc agccagccgg tagtacgtcc gtcgtggaac
     5221 gatccggtgc aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg
     5281 gtacaaaacg ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt
     5341 attcgcggtg tgggcagtgc gctggcccga gcctcgcgct ggaatcgcat tcctgattac
     5401 ctgcaaaaag ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg
     5461 aagaacttac cgcagattct ggacgtggaa ggggtcgacg gcgtgtttat cggcccggcg
     5521 gatctgagcg ccgatatggg ttatgccggt aatcctcagc acccggaagt acaggccgcg
     5581 attgagcagg cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc
     5641 aatgagcaac tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt
     5701 gacaccaccc tgctcgcccg cgccgccgaa gcgctggcag cacggtttgg tgcgcaggct
     5761 acagcgatta agcccgacgt gtattaatgc cggatgcgct gcgcttatcc ggcctacact
     5821 cgcaccaaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa
     5881 atcgcggacc ggataaggcg tttgtgcgca tccgccataa accttgtcgt accctacaaa
     5941 aatcccatta gaggaagtaa aatgagcgac acctcacctg ccataccgga gagtatcgat
     6001 ccggcgaatc agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctattt
     6061 cgccgcctga tcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac
     6121 atcggctttg ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc
     6181 ctcgctacca ccttgttcta cgccgcttac gtcatcttcg gcattcccag caacattatg
     6241 ctgagtattg tcggtgcacg gcgctggatc gccaccatca tggtgctttg gggcatcgcc
     6301 tctactgcca ccatgtttgc cactggcccc accagcttat acgtactgcg tatactggtt
     6361 ggcattaccg aagccggctt tctgcctggc attctgctgt atttaacctt ctggtttccg
     6421 gcctatttcc gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg
     6481 ttgggatcga tcgtttccgg ctacattttg tcgctggatg gcgtaatggc attaaaaggc
     6541 tggcagtggc tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg
     6601 ttctggcttg atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc
     6661 ctgcaagaga tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc
     6721 caccacgcca tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg
     6781 tataccctgg cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg
     6841 cagatcctgc aaagctttaa tcagggcagc agtaatatca ccatcggcct gctggccgcc
     6901 gtaccgcaga tttgtaccat tctcgggatg atctactgga gccgtcactc agatcgccgc
     6961 caggaacgaa ggcatcacac cgcccttcct tatttgttcg ctgccgctgg ttggttactg
     7021 gcttcggcaa ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga
     7081 tcattcagcg caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca
     7141 cgagcgatcg gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg
     7201 tttatgatcg gctggttgaa agatctgacc ggcagcttta acagtggatt gtggtttgtt
     7261 gccacgctgc tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt
     7321 ccgcgagcga ccccgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc
     7381 agcaaagagt acgatgaaag cctgggcacg gacgatgtgc attatcagtc gttcgcccgc
     7441 atggcggcct tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg
     7501 catttcctca atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa
     7561 gcgcccctgt ttgtcctgac gccgccgtca gtacctcatg cgtttattac ggagtctgat
     7621 gctgacggtc atgtattgac ggtacgggaa gatctgatct ggcccctgct ggaagttctt
     7681 tatccgggca ctcgggaaac cttcggcctg ccggggattt gcctgtcact ggcagataaa
     7741 cccgacgaac tggcggcgct ggaacactat tggcaactga tagagcggga atcggtagaa
     7801 caactgcctg gtcgggaaca caccctgacg ttactggcac aggcagtgtt caccctactg
     7861 ctgcgtaacg caaaactcga cgaccatgcc gccagcggaa tgcgcggaga attaaaactg
     7921 ttccagcgtt ttcatatgct gattgaaagc cattttcatc agcactggac agtaccggat
     7981 tacgctaacg aattgcatat caccgaatca cgcctcactg acatctgccg ccgctttgcc
     8041 aaccgtccgc caaaacggtt gattttcgac aggcagctgc gagaagccaa gcggctgctg
     8101 ctgttttctg ataacgccgt gaacaatatt gcctggcaac tcggttttaa ggatccggct
     8161 tattttgcgc gcttttttaa tcgcttagtc ggttgctcgc ccagtgctta tcgcgccaaa
     8221 aaagtacctg tgacgtgaaa atcccttagc cttaacggga aaccaggcac cacctgctat
     8281 tcccctttct ccgcctggag caaggggatt tagtaacgtt aaaaatcgct aaaagcgacc
     8341 tcgatcacaa aaccgtaccc gatctgaaaa gtaccagcca ttgtcccaaa agtgtcttcc
     8401 cgtaatcgcc cctgcaagct tcaattgaat aacaaaaaca acacacaaaa ttaacaatcg
     8461 tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg ccagtaccca
     8521 acgtccgttc accggggaag agtatctgaa aagcctgcag gatggtcgcg agatctatat
     8581 ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg cggcagcgtc
     8641 tgttgcccaa ctgtacgacg cgctacacaa accggagatg caggactctc tgtgctggaa
     8701 caccgacacc ggtagcggcg gctataccca taaattcttc cgcgtggcga aaagtgccga
     8761 cgacctgcgc cagcaacgcg acgccatcgc tgagtggtca cgcctgagct atggctggat
     8821 gggccgtacc ccagactaca aagccgcttt cggttgcgca ctgggcgcga atccgggctt
     8881 ttacggtcag ttcgagcaga acgcccgtaa ctggtatacc cgtattcagg aaactggcct
     8941 ctactttaac cacgcgattg ttaacccacc gatcgatcgt catttgccga ccgataaagt
     9001 gaaagacgtt tacatcaagc tggaaaaaga gactgacgcc gggattatcg tcagcggtgc
     9061 gaaagtggtt gccaccaact cggcgctgac tcactacaac atgattggct tcggctcggc
     9121 acaagtgatg ggcgaaaacc cggacttcgc actgatgttc gttgcgccaa tggatgccga
     9181 tggcgtgaaa ttaatctccc gcgcctctta tgagatggtc gcgggtgcta ccggctcgcc
     9241 gtacgactac ccgctctcca gccgctttga tgagaatgat gcgattctgg tgatggataa
     9301 cgtgctgatc ccatgggaaa acgtactgat ctaccgcgat ttcgatcgct gccgtcgctg
     9361 gacgatggaa ggcggttttg cccgtatgta tccgctgcaa gcctgtgtgc gcctggcagt
     9421 gaaactagac ttcattacgg cactgctgaa aaaatcactc gaatgtaccg gcaccctgga
     9481 gttccgtggt gtgcaggccg atctcggtga agtggtggcg tggcgcaaca ccttctgggc
     9541 attgagtgac tcgatgtgtt cagaagcgac accatgggtc aacggtgctt atttaccgga
     9601 tcatgccgca ctgcaaacct atcgcgtact ggcaccaatg gcctacgcga agatcaaaaa
     9661 cattatcgaa cgcaacgtta ccagtggcct gatctatctc ccttccagtg cccgtgacct
     9721 gaacaatccg cagatcgacc agtatctggc gaagtatgtg cgcggttcga acggtatgga
     9781 tcacgtccag cgcatcaaga tcctcaaact gatgtgggac gccattggca gcgagtttgg
     9841 tggtcgtcac gaactgtatg aaatcaacta ctccggtagc caggatgaga ttcgcctgca
     9901 gtgtctgcgc caggcacaaa gctccggcaa tatggacaag atgatggcga tggttgatcg
     9961 ctgcctgtcg gaatacgacc agaacggctg gactgtgccg cacctgcaca acaacgacga
    10021 tatcaacatg ctggataagc tgctgaaata acgcagcagg aggttaagat gcaattagat
    10081 gaacaacgcc tgcgctttcg tgacgcaatg gccagcctgt cggcagcggt aaatattatc
    10141 accaccgagg gcgacgccgg acaatgcgga attacggcaa cggccgtctg ctcgatcacg
    10201 gatacaccac cgtcgctgat ggtgtgcatt aacgccaaca gtgcgatgaa cccggttttt
    10261 cagggcaacg gtaagttgtg cgtcaacgtc ctcaatcatg agcaggagct gatggcccgc
    10321 cacttcgcgg gcatgacagg catggcgatg gaagagcgtt ttagcctctc atgctggcaa
    10381 aaaggtccgc tggcacagcc ggtgctgaaa ggttcgctgg ccagtcttga aggtgagatc
    10441 cgcgatgtgc aggcaattgg cacacatctg gtgtatctgg tggagattaa aaacatcatc
    10501 ctcagtgcag aaggtcacgg acttatctac tttaaacgcc gtttccatcc ggtgatgctg
    10561 gaaatggaag ctgcgattta agctccagcc ccttcacgca ttcgtcgaag gggcattttt
    10621 attgtgatct caatcacata tccacccgat gaataattct tacccaaccg ctcaccgcca
    10681 ttcctgaccg cttacatccc taaaataacc actcagttat ttaccttact ttacgcgtgc
    10741 gtaactctgg caacatcact gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa
    10801 acctagaacg gcttcggcct atgattaaca aattactcta tggattttgg tttgcaggaa
    10861 ggcagcaagt gagtgaatcc ccgggagctt ataacagtaa gtgacagggg tgaacgaacg
    10921 cagctgccgc acctgtaagc caaaagacga cgagtaaaat gccaggtttt actatggata
    10981 cgaaaaaact attcaagcac ataccctggg tgattctcgg aatcatcggt gcattctgcc
    11041 tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc gtggtcgcct
    11101 ctgtgtcggt gtatctggtg gcgtatcgct actacagtct gtatatcgcc cagaaggtga
    11161 tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt ctgaactacg
    11221 ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc ggtgctggtc
    11281 cgctggttgg tccggttctc gccgcgcaga tgggctacct gcccggcacg ctgtggctgc
    11341 tggcgggggt agtgctggcc ggtgcggtac aggactttat ggtgctgttt atctcctctc
    11401 gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga gatgggacca gtaccgggga
    11461 ctatcgcgct gtttggctgt ttcttaatca tgatcatcat ccttgccgtc ctggcgctga
    11521 ttgtggttaa agccctggca gaaagtccgt ggggtgtctt caccgtttgc tcaaccgtac
    11581 cgattgcgct gtttatgggt atctacatgc gctttatccg tccggggcgt gtgggtgaag
    11641 tctctgtaat cggtatcgtg ctgctggttg cctctatcta cttcggtggc gtgattgctc
    11701 acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc ttcgcgctga
    11761 ttggctatgc gtttgtttcc gcactgctgc cggtgtggct gatcctcgca ccgcgcgact
    11821 atctggcaac cttcctgaaa atcggcgtta tcgtcggcct ggcgttgggt atcgtggtgc
    11881 tgaacccgga actgaaaatg cctgcgatga cccagtacat tgacggtact ggcccgctgt
    11941 ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg gtatctggct
    12001 tccacgcgct gatctcttcc ggtacgacgc cgaaactgct ggctaacgaa accgacgcgc
    12061 gtttcattgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg gcgctggttg
    12121 cagcgtccat catcgaaccg ggtctttact tcgcgatgaa caccccgcct gcgggccttg
    12181 gcatcaccat gcctaacctg catgaaatgg gtggcgagaa cgcgccgatc atcatggcgc
    12241 agctgaaaga cgttaccgca cacgcggcgg cgaccgtcag ctcctggggc ttcgtgattt
    12301 cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc ctgaaccgtg
    12361 caggtggtgc gccgacactg gcggtaggta tcgcgcacgt gttccacaaa gtgctgccga
    12421 tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg ttcatcctga
    12481 ccgcgctgga tgcgggtacc cgttctggcc gctttatgct gcaggacctg ctgggtaact
    12541 tcatcccgtt cctgaaaaaa accgactctc tggttgctgg tatcatcggt actgcgggct
    12601 gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg ggcggcgtta
    12661 agagcctgtg gccgctgttc ggtatctcca accagatgct ggcagccgta gcgctggtac
    12721 tgggcaccgt tgtgctgatt aagatgaagc gcacccaata catctgggta actgttgttc
    12781 cggctgtatg gctgcttatc tgcaccacct gggcgctggg cctgaaactg ttcagcacca
    12841 acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag attgctaacg
    12901 gtactgacct gacggcacag cagattgcca acatgaacca catcgttgtg aacaactaca
    12961 ccaacgcggg cctgagtatt ctgttcctga ttgtggtgta cagcatcatc ttctacggtt
    13021 tcaaaacctg gcttgcggtg cgtaacagcg acaaacgtac tgacaaagaa acaccgtacg
    13081 ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccggtt gccggatgcg
    13141 ttgcgcgtat ccggcctaca aagtgtctat cgtaggccgg ataaagcgtt tacgccgcat
    13201 ccggcaaatc cctagcccgg accttccggg ctttacgcat atctggaagg caaaatgttt
    13261 ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggcatt
    13321 ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg
    13381 agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc
    13441 ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg
    13501 ttttttaggc gcgggcaaaa ccaccctgct gcgccatatt cttaacgaac aacacggcta
    13561 caagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc aactgattgg
    13621 cgaccgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa
    13681 cgagctggaa gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt
    13741 cgaccgtctg gtcattgaat gcaccggcat ggccgatccc ggtccgatta ttcagacctt
    13801 tttctcccat gagattttat gccagcgtta cctgctggac ggcgtgattg cgctggtgga
    13861 tgcggtacat gccgatgagc agatgaacca gttcaccatc gcccagtccc aggttggcta
    13921 cgccgaccgt attctgctga ccaaaaccga cgtcgcaggc gaagcagaaa aactgcgtga
    13981 acgcctggcg cgcatcaacg cccgtgcacc ggtctacacc gtcacccacg gcgacatcga
    14041 cctgggcctg ctattcaaca ccaacggttt tatgctggaa gagaacgtcg tcagcaccaa
    14101 accgcgcttc cactttatcg cggataaaca aaacgatatt tcgtcgattg tggtggaact
    14161 ggattacccg gtagatatca gcgaagtttc ccgggtgatg gaaaacctgc tgctggaatc
    14221 ggcagataaa ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct
    14281 gctattccag ggcgtccagc gcctctacag cgccgactgg gaccgcccgt ggggcgatga
    14341 aaaaccgcat agcacgatgg tgtttatcgg gattcagctg ccggaggaag agattcgggc
    14401 ggcgtttgcg gggttgagga agtaatcgtg aaaggttgag tgtaaagcag ctcaatgccc
    14461 catttagcag tgagggggga aacagaacga cgtgctcccc tccgctaagt tttattgcgc
    14521 gtgaatacct ggatctggca aaaaattgcc gcgtttggcg tcagaccatg cttcgctacc
    14581 agaagcagga atgtcgctgt aatcaatgtc ttcgtcggat ttattaacca aagccatgag
    14641 ttcagcttct tgctgcacat caagtgtgta gagtgtgccc cgtttatgtt taaccatgct
    14701 catttatgac cagaaccttt acgatttaat tataaagatg acttatttca gccatccagg
    14761 cacgttatat tctgagacat tctcaatgga atatgcatct ctcctgtcag aacctttctt
    14821 acacttcaac tcattgaccc cccatttacc ctctctattg gaataaaata cagtatattt
    14881 atcggtatta aacccgaaat atctttcatc cggacgtgaa atattcaact ctccacattt
    14941 aacctgatta cgccattcaa aatctatggt gctttttaaa acgtagtacg aaataccttc
    15001 tacatttttt gaagcaaaag ccactaaaat agtagatacg ataa
//
LOCUS       DAZKGS010000110        15030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKGS010000110 DAZKGS010000000
VERSION     DAZKGS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1074
                     /locus_tag="ACHOQY_003343"
                     /pseudo
     CDS             1..1074
                     /locus_tag="ACHOQY_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
     gene            1275..1526
                     /locus_tag="ACHOQY_003344"
     CDS             1275..1526
                     /locus_tag="ACHOQY_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679389.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            1523..1933
                     /locus_tag="ACHOQY_003345"
     CDS             1523..1933
                     /locus_tag="ACHOQY_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9679390.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            1944..2216
                     /locus_tag="ACHOQY_003346"
     CDS             1944..2216
                     /locus_tag="ACHOQY_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9679391.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            2173..2301
                     /locus_tag="ACHOQY_003347"
     CDS             2173..2301
                     /locus_tag="ACHOQY_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9679392.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            2343..2567
                     /locus_tag="ACHOQY_003348"
     CDS             2343..2567
                     /locus_tag="ACHOQY_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679393.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            2819..3025
                     /locus_tag="ACHOQY_003349"
     CDS             2819..3025
                     /locus_tag="ACHOQY_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679394.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQTHGGE"
     gene            3025..4080
                     /locus_tag="ACHOQY_003350"
     CDS             3025..4080
                     /locus_tag="ACHOQY_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9679395.1"
                     /translation="MAGKATALNTNQLFAYLNRGDIAEFKFSPLFTTLFFPNVATFNT
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNLIEGEGIERYE
                     IDWKIPESNIIEQADGKKWSELDKETHDPIYDIELYADQGNCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALVVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            4093..4428
                     /locus_tag="ACHOQY_003351"
     CDS             4093..4428
                     /locus_tag="ACHOQY_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9679396.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLAENGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESADTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            4441..4854
                     /locus_tag="ACHOQY_003352"
     CDS             4441..4854
                     /locus_tag="ACHOQY_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP9679397.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNSLEEKD
                     KPRADAIMALHESDDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            5060..5602
                     /locus_tag="ACHOQY_003353"
     CDS             5060..5602
                     /locus_tag="ACHOQY_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9679398.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDILPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            5858..6139
                     /locus_tag="ACHOQY_003354"
     CDS             5858..6139
                     /locus_tag="ACHOQY_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679399.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(6741..8201)
                     /gene="phoQ"
                     /locus_tag="ACHOQY_003355"
     CDS             complement(6741..8201)
                     /gene="phoQ"
                     /locus_tag="ACHOQY_003355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9679400.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8201..8872)
                     /gene="phoP"
                     /locus_tag="ACHOQY_003356"
     CDS             complement(8201..8872)
                     /gene="phoP"
                     /locus_tag="ACHOQY_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9679401.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(9040..10410)
                     /gene="purB"
                     /locus_tag="ACHOQY_003357"
     CDS             complement(9040..10410)
                     /gene="purB"
                     /locus_tag="ACHOQY_003357"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9679402.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(10414..11055)
                     /gene="hflD"
                     /locus_tag="ACHOQY_003358"
     CDS             complement(10414..11055)
                     /gene="hflD"
                     /locus_tag="ACHOQY_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9679403.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(11091..12197)
                     /gene="mnmA"
                     /locus_tag="ACHOQY_003359"
                     /gene_synonym="trmU"
     CDS             complement(11091..12197)
                     /gene="mnmA"
                     /locus_tag="ACHOQY_003359"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9679404.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(12251..12712)
                     /gene="nudJ"
                     /locus_tag="ACHOQY_003360"
     CDS             complement(12251..12712)
                     /gene="nudJ"
                     /locus_tag="ACHOQY_003360"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9679405.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(12722..13375)
                     /gene="rluE"
                     /locus_tag="ACHOQY_003361"
     CDS             complement(12722..13375)
                     /gene="rluE"
                     /locus_tag="ACHOQY_003361"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9679406.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            13547..14797
                     /gene="icd"
                     /locus_tag="ACHOQY_003362"
     CDS             13547..14797
                     /gene="icd"
                     /locus_tag="ACHOQY_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9679407.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(14900..>15030)
                     /locus_tag="ACHOQY_003363"
     CDS             complement(14900..>15030)
                     /locus_tag="ACHOQY_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9679408.1"
                     /translation="PFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
BASE COUNT         3942 a         3632 c         3947 g         3509 t
ORIGIN      
        1 atgcgggaga actggcgcga atacctggaa cgggtgcgcg ggaaagagcc ggcggaagaa
       61 aaaaaccacc agcgaaaaac cacgctaccg atgagcgttg gatctaccgg atacgacacg
      121 caactggatt acgtggttaa ggggattatt ccggcggtat cgctatgcag catatacggg
      181 gctagcgggt cctataaatc attccttgcc ggatcgtggg cgtgccatgt tgccactggt
      241 cgccagtggg gaggccgcag ggttgcacat ggtgcggttc tctatgtggt tggtgaaggc
      301 ggtataggtg ttccgcgtcg tgtaaaagcc tgggaggttg tgcacgatga gtaggtgaaa
      361 aatctgtatc tggtaaaccg ccccatcttt ccggctgccc cgcttgatgt tgatgaaatg
      421 gttatcgctg cccgtcaggt ggagcgggaa acgggtaaac ctgtacgcat gattattctg
      481 gatacgctgg cgcgttgctt tggtgggaat gatgaaaatg attcccgtga tatgggggcg
      541 tttatccgtg gttgtgacga actgaaacga cgcacagggg ccacggtgct ggtggttcac
      601 cattccggca aggatgagac gaaaggcgcg cgcggttcca gtgcatttcg tgcttcgctg
      661 gatgctgaat accggatacg cagggaggac gcaggaagcg aagcgctggt tatctcatgc
      721 accaaaatga aggacgcgga ggaactcaaa gaagccgcat atgacttacg cgtggtggag
      781 ctttttaccg acgctgacgg tgaattaatc acgtcgctgg tggtggtgga tgatccgcgc
      841 cctcctgttg aactggagcg catcgaggag gcagggaaca agacggaaaa ccataccgcg
      901 ctatgggggt gcatccgttc acgcacacag aacggcgaca agtgcacgat cccgctgtta
      961 cgtgatgaca tgaaaaagct ggggtatgaa atgaaaaact tccggcgctg gctgtacaag
     1021 ctggaaaaag atggggttat tcgtatcgat ggggatgatg tagcgccgct ataaaagtga
     1081 ggagcaaaag cgagggggat agaaagaggg ccaaaattag cccgctctcc ctcacttttc
     1141 gacctgtata catcctcaaa agtgaggggt aaaaaaatac ttatgaaaca cacacataga
     1201 aaaaccgaaa atcccaactg cgacgaagtg agacgcttga aaaagtgagg cgaaaaagtg
     1261 agaggttgcg agaaatgacc caaaaacgca gagacagaac agagccaaaa tataaagcgt
     1321 tagacatgac tgagcacacc ttaaaggtgg caatcagaac gatagaccgc cacacgcggg
     1381 aaggatacgc gaaggaacat cccgacctga taagcgcatt catgaccacg gcggcggcaa
     1441 actttgccac gctgacagaa cgggagattg ccgaagcgga acaggtaaca accatcaacg
     1501 ttaaaaccgg agaggtggaa ttatgacggc acagatagcc gcttacgggc ggctggtgga
     1561 cgacccgcag gtaaaacaga ccagcaaggg cacaccgatg acgctggcgc gtatggcggt
     1621 atctttgcca tgcagccagg cacaggacgg acaggcgacg ttatggttat cggtcatcgc
     1681 atttggcaaa caggccgact tcctggctaa acatcaaaaa ggcgatgttg ccagcgtatc
     1741 cggcacgatg caggtaagcc agtggaccgg acagaacgga gaaacgcggc agggctggca
     1801 ggttatcgca gacagcgtaa tcagtgcccg cgcggcacgt ccaggcggga acagacgcaa
     1861 aaccacaggc acacagggta atcagccacc agcgggaggc gatgaccctt acggtgatga
     1921 tatttcgttc tgagggggtg acgatggtac atgaccgcat agcggaggaa ctagaggcga
     1981 aaggctttta ccggagggcg gcggcgcgat ggggtgaagt catgcagctg gtggagacag
     2041 acaaggaacg gcatcacatc acgatgcgac ggctggaatg ttcaaggaag gcacagaggg
     2101 caccggagcc gccggataat ttcggagacc tgaaaaaggc agtcgatcgc acttatgccg
     2161 aaatgggtat agatggtgct ggtgatgaaa tatggcgcaa ttaccaggac agctaatcaa
     2221 acagccggag aaatccggct ttttttgcac cagttgaaac ggtatggcgc attaccgggt
     2281 tttcgtcacg gtcaggcata gttactatct gaaacaaaca gacacaacag aggaaaaaaa
     2341 caatgccgat gaaatttgat gagatattaa aacagcgtga taaataccat gctgacaaca
     2401 tggagacgat gagcatcaat gattaccgcg cattcctgga gacgggcgca ctgattgaaa
     2461 aggatcagca tggttttgtg agatgtgctc tatccggtga aatgctggcg gtaaatcctg
     2521 aacagataga tgcattgata gaatttctga aagagatcag agactgagcc agcacacagc
     2581 acacatagcc ggagcaatcc ggcttttttg cgcccaaaaa aagcccgata aggtcagagg
     2641 gttcttatcg ggcttttgca tatgaggttt ttggatgcac tggcgttcgt gatcgggata
     2701 atcatttcat aatttgcaac ataactcaat atcattgcat aaaatgcaat tctgattata
     2761 atcaggactg gataaacatc cagttatgat tttttaagtc gaagaggaat ttcttactat
     2821 ggctgaagag aaaaaaggcg gtgtttcggt gtacataagc cccgacatcg tgaaggcgct
     2881 caaggaacgc caccagcaga acgtaaaagc aggcattgcg gcaggacttg atccgctggc
     2941 gatggttgag ccgtcaacag gctggcaggt ccgcgcctat ttacgcgcgg cgctgggcat
     3001 gaatcagacg catggaggtg aataatggca ggcaaagcaa cggcacttaa cactaaccag
     3061 cttttcgcgt acctgaatcg cggggatatt gcagaattta aattcagtcc gctgtttacc
     3121 acgctgtttt tcccgaacgt ggcgaccttc aacacccaga acatcatgct ggacaccctg
     3181 gacattgaag aggttacgat gtcagcgttc tgttcgccta tggtgggtag tcaggtccag
     3241 cgcgataaag ggtacgaaac cagcacgatt aaaccaggct acatgaaacc caaacacgaa
     3301 atcgatccaa caaaaaccat catgcgcatg gctggagaag atccggcaca gcttaacgat
     3361 cctacctatc gccgtatgcg cctgattact ggcaacatgc gccgccagat aaacgccatt
     3421 aaggcacgcg tggaatggct ggcggtgaat gcggtaacga ccggaaaaaa cctcattgag
     3481 ggcgaaggca tagaacgcta tgaaatcgac tggaagatcc cggaaagcaa tatcatagaa
     3541 caggctgacg gtaaaaaatg gtctgagctg gataaagaaa cacacgatcc aatctatgac
     3601 atcgagcttt atgctgatca gggcaattgc cccgcaaacg tcatgattat gggcgctgaa
     3661 gtatggcgca cgttacgcag ctttaaaaaa ttccgcgaac tgtacgacct ttcccgtggt
     3721 tcagaatccg ccgcagaact ggcctgtaaa aacctgggtg aagtggtgag ctttaaaggc
     3781 tatctgggcg atattgccct tgtcgtctat tccggcaaat acactgacag cgacggcacc
     3841 gaaaaatatt tccttgagcc tgatttgctg gtcctgggta acaccaacaa taaagggctg
     3901 gtggcctatg gtgcgattat ggatcaggaa gcggtcagaa cgggcgcaac gcaaaacatg
     3961 ttttacccga aaaactggat tgaggacggc gatccggcga ttgagtacgt gcagacgcac
     4021 agcgcaccgc agccggtacc ggcagatatt cgcaaatttg ttaccgtcaa aattggttaa
     4081 cggggggatt ctatggacac tccatacatt gagttatttg caggtagtca gcaggtatcc
     4141 acgacgctgg tacattttgc tgctgatgct ggcgttattc aggaatttac cccgctgatg
     4201 ctggcggaaa atggcgagtt taagccgtgg gatggtcagg aatctggcaa ggctgtttat
     4261 ctgacctcgt accccgtgga cacgtcgacg cagaaatcag cacagtgcta caagacgggg
     4321 atattcaata ttgccgccgt taactggcct gagagcgccg acaccgacgc gaaaaaatgc
     4381 gccgcctttg cgggttctgg cgtatccgtt cagccgctgg cgcgataagc agggggaacg
     4441 atggcaacga atgaaagtat catgacgcta ccgctggcga gtaaatttaa agccgaagcg
     4501 cgggcaatgg ctgacagagg tttatcaacc tacgaggccg tatatcaact taacagcctg
     4561 gaagagaagg acaagccgcg cgctgatgcg attatggcgc ttcatgaatc tgacgactat
     4621 cagccgctgt tacgtgcaat ggcaaacgtg ccttgtattg atgttgatac ggctaaaaac
     4681 atcctgaaca tgaccataga gcaggaacgc ccgaaggttg caccagagct taccgcagcc
     4741 tttgaaaact ttatggacat gcacagccca caagccgtat cagctggcat ggcatacgat
     4801 ggcagaaacc cgggcgatga cggcgacatc gatcgcatac tgaaaaccat ctgagacaag
     4861 gccggagaaa tccggctttt ttgcgggtcc tttccggcat atggacctgt tacggggcgg
     4921 agacctcgcg gattttcgct atttatgagc cttttcaggg gggtggtggt ggttttgttg
     4981 tttgatctat ctttatgaat gaaaagggaa agatgcaagc aatacaccaa cctgaagcag
     5041 taattaagtt ggtgtattaa tgaaatctca tctgatgaac aaaaaaaaca tggcgaaaag
     5101 ctgccgtgta agtgcgacag cgttcgacaa gtggggagta actcccgttg agcgtaaagg
     5161 ccgtgaggcg ttttatgatg ttgccagcgt gatagaaaat agggttaaca atgcaattag
     5221 ccagcttata aacgacaaag gcgagattga cgatgatgaa cttttacgag tcaggatcag
     5281 attactgaca gcacaggcag aggcgcagga acttaaaaac gatcgcgatc gcggtgacgt
     5341 gattgatact gagttttgcc tgtacgcgct ttcaaagctg gcgagtcaga tttcatcaat
     5401 catggacatc ctcccgctta ctatgcaaag gagcttccca cagattaccc ccgccatgct
     5461 ggacaggctt aaaagggaag tggttaaagc ctgtaatgcc agtgccagag ttgccgacaa
     5521 cctcccacag atactggctg attacttgaa agaaacaacc ggaaacgtac cggaaaagtt
     5581 gctacagaag aaaggcgagt aacagacgcg caattattga acaaaaatga gaaaggatct
     5641 gataatggca ttttctggct aaaatgcctc gtgtcagatc ctttcttttt ttaatatttt
     5701 cattgttaaa cagtctgtta cagagaagaa gatcggatct gacttttgcc ggaaaattct
     5761 cataaacaga taaaaaccgc gaggtcgccg ccccgtaacg ggccataatt ccaggaagga
     5821 cccgacgaca ccagactatc agagcgatgg gggcacaatg acagaagccg aactactggg
     5881 attaatccgc cgcgtcgccg gaatcagcca gcagcatgac gaacaggcca cacagccgga
     5941 cagcgtgacc gctgaaaatt atgtgcgtgt tgtagcggaa gtgatgcgcc gtgatggtat
     6001 agaacttaat ggcgttgata tgcgcaacat acggaaccgc gttcttgaaa tgctggccta
     6061 caatcgccgc gttgaactgt atcgggagaa agaaaaaata acgtaccact ggaagaagcc
     6121 ggaacggctg cggcggtaac ttgctgatat tcccgataac gcaaaattgc gctggctaca
     6181 tccctcacat ctgaggactg ttaaccagaa ggccggaata tattcacaaa ctacgaaatt
     6241 ttcgtagttt aattaatggt cagattaatc gctaacccgc tgatattttc ggaaacctca
     6301 atttgaggaa gtcggaagcg tcacccttca tattctgaat ggtcataatg accacgcaga
     6361 ccatcaaggc aaccacaacg aacatcgtta cggttggagc ttttacccca ctgggggaaa
     6421 ggtattacga taatcataac acctaccgaa caggtaaaac ccaccagcct gattaacagt
     6481 taaccggaaa aaaaatccgg tagccccgtt atggggatcc ccatatcgac attaacgccc
     6541 ctcatgaatt ggtgcgcttc cccctggaaa gattacccgc ctgtatattt cttgtgtcta
     6601 tttgttccac gttgtttcat acagtgcacc gaacggtgta gttaatggtg tagtcaattt
     6661 gctgttttta gcactttttg cagtgtgtgt tgtttaatta aatcaatggg ttgccttgct
     6721 gtggtgataa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     6781 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     6841 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     6901 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     6961 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     7021 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     7081 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     7141 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     7201 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     7261 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     7321 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     7381 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     7441 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     7501 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     7561 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     7621 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     7681 gttaattttg gcatccgcga tgttgccggg tagacgttta ctgccaccga gtgggtcatc
     7741 tccgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     7801 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     7861 agccagtcag gctggatcat cttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     7921 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     7981 aactcgacat gcaacttatt gttttcccac ttcgcaaggg tatagaacag attgctctcg
     8041 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     8101 attccgtagg caagcgaaag caccagtact accgcagccg ttgccaacag aaaacgtacc
     8161 cgcagtgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaaat
     8221 agccctggcc gcgaacggta gtaatcactt cttgcggata ttgtgcctga atttttttgc
     8281 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     8341 gttggagcat taacgaatct ttgctcacca ctttgccatt attgcgtatc aacgtttcca
     8401 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     8461 gagagagatc aacctgaaac gggggtagcg aaatgacctg tgaagccagg ccgctattac
     8521 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     8581 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     8641 ataccagaat cggcagtgaa acatcattgc tacgccagcg gcgaatcagt gacagaccgt
     8701 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     8761 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     8821 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     8881 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     8941 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata attttaataa
     9001 acaggccgga cagcatcgcc atccggcact gatacgagtt tatttcagct catcaaccat
     9061 ggtgatagct cgaccaatat agttagccgg cgtcatcgct ttcaggcggg ctttctcttc
     9121 ttctggcaac gccagaccat cgataaactg cttcatgcct tcggcgtcaa cgcgcttacc
     9181 gcgagtcagc tctttcagct tctcgtacgg tttttcgatg ccatagcgac gcataactgt
     9241 ctggattggc tcagccagca cttcccagtt gtgatccagc tcatccagca gatggtcacg
     9301 gtttacttcc agtttgctca cgcctttcag ggtggattga tacgcaatca gcgcataacc
     9361 gatacccacg ccgaggttac gcagcacggt ggagtcggtc aggtcacgct gccagcggga
     9421 aaccggcagt ttgcttgcca gatgctgcaa taccgcgttg gaaaggccca gattcccttc
     9481 ggagttttcg aagtcaatcg ggttaacttt atgcggcatg gtggaagaac caatctcacc
     9541 agcaatggtt ttctgtttga agtggttaag ggcgatataa ccccagacgt cacggtcaaa
     9601 gtcgatcaga atggtgttga agcgcgcaac gcaatcaaac aattcggcaa tgtagtcgtg
     9661 cggttcgatc tgggtggtgt acgggttcca ctgaataccc agcgaggtga cgaactcttc
     9721 gctgaactga tgccagtcaa cttccgggta agcggcgata tgggcgttat agttaccgac
     9781 cgcgccgttg attttgccga ggatctccac ctgattaagc tggcggtact ggcgctccat
     9841 acggtaggcg acgtttgcca tctctttacc gatggttgac ggcgtggctg gctgaccgtg
     9901 ggtacgagac agcagcggga tatcgcggta ctgaacggcg agatctttaa tgccatcaat
     9961 cagttgacgc cagtacggca ggatcacttc atcacgcgcg gttttcagca ttaatgcgtg
    10021 ggagaggtta ttgatatctt ccgaagtaca ggcaaagtgg atgaattcag aaaccgcgtg
    10081 cagttccggg atctccgcca ctttttcttt caggaaatac tcaaccgctt taacgtcgtg
    10141 gttagtggta cgctcgatgg ttttgatgcg tgcggcatct tcttcgctga aattggcgac
    10201 aattgcatca aggtaaccga ttgcgtcggc agcaaaagca ggaacttcct tgatcgctgc
    10261 gtgcgcggcc agtttttgca gccaacgtac ttcaacttgt acacggaatt tcagcaaacc
    10321 atattcgctg aaaatcccgc gcagcgcgct gactttatcg ccgtagcgtc catcgacagg
    10381 ggaaacggcg gtcagtgagg ataattccat agatcacaac tccggggtta aatgagcaag
    10441 aatttgtttt gcctgagtgg tcaggcgatt acgagaaaac atcagttgca gacgtccgcc
    10501 gccgacctgg tgccagagca cggcggcgcg aatgcctgcc agcagggttg cgcgaacttt
    10561 cgcctgcact tgtgggcttt gcagtacagc aggggaaccg gtgacctgaa tgcgcgggcc
    10621 aagcgggctg atcacatcaa catagatagc agccatcgcg ctcatcagcg tttcggactg
    10681 taaatcgaag tgttcgagct ggcgttgcag gccgttgatc cggttgccca gagtgtcgag
    10741 cgcgcctttc gctgaggaga gtttgcgctc aagcaccatc aagctgagtg tgtagcgggt
    10801 taattcggcg tttaagccct ggcgactgct ggcattgagc acgccgagca gggtttccag
    10861 cccgacgcgc aggttggctt cgctaccgcc aaaaaccgcc agcgtcgagc tggggttcat
    10921 atcaataata ctgttgagtg agacgtgtag cgcatcggca tcacaatgcc cctggtgagc
    10981 gagttgttgc accaggcgtg ccgactgaca aataccggcc agggcgaggg tgatgtcata
    11041 gtaattcttt gccacgttca ctgcttcctt gttttaagta aagataataa tcagaccggc
    11101 agcggcagac gctgctcaat aataccgcca ccgaggcaca cttcaccgtt atagaagacg
    11161 gcagactggc ccggcgtcac ggcggcaacc ggttcatcga aaatcacttc aatgcgatca
    11221 tcgtccagcg ccttgacggt gcaagggata tcggtctggc gatagcgggt ttttaccgtg
    11281 caacgcatag tgccggtgaa tggttcgcga tcgacccagt gcaactgctg ggcaatcaac
    11341 ccgacagaca tcagccgcgg gtgttcatgg ccctgagcga caaccagaat gttgttttcg
    11401 acgtctttgt ccaccacata ccacggttct tcggtacctt ctttggtgcc accgataccc
    11461 agacctttac gctgaccgag agtgtgatac atcagcccct ggtgctcacc aatttcatcg
    11521 ccatcgacgg taatgatttt gcccggttgc gccgggagat aacggcccag gaactcgcgg
    11581 aatttacgtt cgccaatgaa gcaaatgccg gtagagtctt ttttcttcgc ggtgaccaga
    11641 ccaagatctt cagcaatctt acgcacctgc ggtttttcca gttcgccgac cgggaacagg
    11701 ctttgcgcaa tctgctcatg gctgagcgta taaaggaagt agctctggtc tttattgctg
    11761 tccagaccac gcagcaggcg gctcttgcca tcgacatcgg cccgacgtac gtaatgaccg
    11821 gtagcgatat aatcggcacc taaatcttcg gcggcaaatt cgaggaaggc tttaaatttg
    11881 atctctttgt tgcacagaat atccggattc ggcgtgcgac cggctttata ttcggcaagg
    11941 aacagttcga agacgttgtc ccagtactcg gcagcaaagt taacggtgtg cagttcaatg
    12001 ccgagcttgt cgcagacagc ctgggcatca gccagatccg ctgccgctgt gcaatattcc
    12061 tcaccgtcgt cttcttccca gttcttcata aacaggcctt cgacctgata tccctgttgt
    12121 tgcaacagcc aggcagaaac ggaggaatcg acaccgccgg acatgccgac gattactttt
    12181 tttgcggttt cagacattgg atcactcacg acattgaact tcaaggcggc gtattctatc
    12241 acgcagcctt ttagatgaca ccctttgtaa aaggccagtt aaaatcgcca atcatctcca
    12301 gcggatagcg ttgcccgctt tgataacaac ggatactttc cgccaccagc ggcgaacgaa
    12361 gatttgacgc ctgtaaaatt tcttcggcgc tgacccaacg acagcagtcg atatcgctgt
    12421 catgaggctg ggtcgggcat atttgctcaa gctcaatggc aaagaggaaa cgcaaaaacg
    12481 gcgttttatc tggcgcaatc cactgatgca tacgaataaa gtgttgcggc tgcgcgctga
    12541 tgccggtttc ttcccacagt tcacgggcgg cggcttccac taaggtttca tcggcttcca
    12601 gatgcccggc aggttggttc cataacgctt taccattaat cgtctcttca acgactaaaa
    12661 atttgccttc tgcgtgcacc acgcaagcaa cggtaacgtg cggtttaaac attttgcatc
    12721 cttaatctgt cacttctcgc cattcgccat tggcaagatt atccaaagag taatctccca
    12781 tcgcatagcg aatcagtcgc agcgtgggga aaccaacatg ggcggtcatg cggcgcacct
    12841 ggcgattacg tccttcatat aaggtgatct tcagccagct ggtgggaata cttttgcgtt
    12901 cacgaattgg tggattccgc ggccataacc acgcgggttc gtcaaccagc tccgcgcctg
    12961 cgggcagggt agggccatca tttaaggtta cgccattgcg caaggcttca agtgcgtctt
    13021 gtgtgggaat accttccacc tgcacataat agattttccc ggtgcgtttg cccggctggg
    13081 ttaaacgcgc ctgcaacgcg ccgttattgg tcagcaccag taacccttcg ctatcgcggt
    13141 caagacgacc tgctgcataa acgccctgaa ccgggatgaa ttcttttaat gttttgcgtc
    13201 cggcttcatc ggtgaactgc ggaagaacat cgtagggttt attgaacagg atcacacgcg
    13261 tgggctggtt ttcaggttta cgcctggtag aacgttgcga gctgaatcgc ttaacttggt
    13321 gatttctaaa agaagttttt tgcatggtat tttcagagat tatgaattgc cgcattatag
    13381 cctaataacg cgcatctttc atgacggcaa acaatagggt agtattgaca agccaattac
    13441 aaatcattaa caaaaaattg ctctaaagca tccgtatcgc aggacgcaaa cgcatatgca
    13501 acgtggtggc agacgagcaa accagtagcg ctcgaaggag aggtgaatgg aaagtaaagt
    13561 agttgttccg gcacaaggca agaagatcac cctgcaaaac ggcaaactca acgttcctga
    13621 aaatccgatt atcccttaca ttgaaggtga tggaatcggt gtagatgtaa ccccagccat
    13681 gctgaaagtg gtcgacgctg cagtcgagaa agcctataaa ggcgagcgta aaatctcctg
    13741 gatggaaatt tacaccggtg aaaaatccac acaggtttat ggtcaggacg tctggctgcc
    13801 tgctgaaact cttgatctga ttcgtgaata tcgcgttgcc attaaaggtc cgctgaccac
    13861 tcctgttggt ggcggtattc gctctctgaa cgttgccctg cgccaggaac tggatctcta
    13921 catctgcctg cgtccggtac gttactatca aggcactcca agcccggtta aacaccctga
    13981 actgaccgat atggttatct tccgtgaaaa ctcggaagac atttatgcgg gtatcgaatg
    14041 gaaagcagac tctgccgacg ccgagaaagt gattaaattc ctgcgtgaag agatgggggt
    14101 gaagaaaatt cgcttcccgg aacattgtgg tatcggtatt aagccgtgtt cggaagaagg
    14161 caccaaacgt ctggttcgtg cagcgatcga atacgcaatt gctaacgatc gtgactctgt
    14221 gactctggtg cacaaaggca acatcatgaa gttcaccgaa ggcgcgttta aagactgggg
    14281 ctaccagctg gcgcgtgaag agtttggcgg tgaactgatc gacggcggcc cgtggctgaa
    14341 agttaaaaac ccgaacaccg gcaaagagat cgtcattaaa gacgtgattg ctgatgcatt
    14401 cctgcaacag atcctgctgc gtccggctga atatgatgtt atcgcctgta tgaacctgaa
    14461 cggtgactac atttctgacg ctctggcagc gcaggttggc ggtatcggta tcgcccctgg
    14521 agcaaacatc ggtgacgaat gcgccctgtt tgaagccacc cacggtactg cgccgaaata
    14581 cgccggtcag gacaaagtaa accctggctc tattattctc tccgctgaga tgatgttacg
    14641 ccatatgggc tggactgaag ccgcagacct gattgttaaa ggtatggaag gcgcaatcaa
    14701 tgcgaagacc gtaacctatg acttcgagcg tctgatggaa ggcgctaaac tgctgaaatg
    14761 ttcagaattt ggtgatgcga tcatcaagaa catgtaatca ctacatgtgt ttaatattgc
    14821 aacgggcgta taacacgccc gttgttttat ttatgtaggt attattaata gcatatcgag
    14881 catatttata taaaaccctt tacttgagcc catatgggca tatttttata atgcaactat
    14941 tatgcaaaca tttatttgtt attttgcttt ctcctggagg acactcttga ctgcttttga
    15001 gtagactcca taaatccttg ttgaatggtg
//
LOCUS       DAZKGS010000111        15019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKGS010000111 DAZKGS010000000
VERSION     DAZKGS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            108..350
                     /gene="yjbE"
                     /locus_tag="ACHOQY_003364"
     CDS             108..350
                     /gene="yjbE"
                     /locus_tag="ACHOQY_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9679409.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            464..1102
                     /gene="yjbF"
                     /locus_tag="ACHOQY_003365"
     CDS             464..1102
                     /gene="yjbF"
                     /locus_tag="ACHOQY_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9679410.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1099..1836
                     /gene="yjbG"
                     /locus_tag="ACHOQY_003366"
     CDS             1099..1836
                     /gene="yjbG"
                     /locus_tag="ACHOQY_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9679411.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1836..3932
                     /gene="yjbH"
                     /locus_tag="ACHOQY_003367"
     CDS             1836..3932
                     /gene="yjbH"
                     /locus_tag="ACHOQY_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9679412.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVVQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3979..4257)
                     /locus_tag="ACHOQY_003368"
     CDS             complement(3979..4257)
                     /locus_tag="ACHOQY_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679413.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4258..4401
                     /locus_tag="ACHOQY_003369"
     CDS             4258..4401
                     /locus_tag="ACHOQY_003369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679414.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4527..4937
                     /gene="psiE"
                     /locus_tag="ACHOQY_003370"
     CDS             4527..4937
                     /gene="psiE"
                     /locus_tag="ACHOQY_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9679415.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4981..6456)
                     /gene="xylE"
                     /locus_tag="ACHOQY_003371"
     CDS             complement(4981..6456)
                     /gene="xylE"
                     /locus_tag="ACHOQY_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP9679416.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6828..7718)
                     /gene="malG"
                     /locus_tag="ACHOQY_003372"
     CDS             complement(6828..7718)
                     /gene="malG"
                     /locus_tag="ACHOQY_003372"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9679417.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7733..9277)
                     /gene="malF"
                     /locus_tag="ACHOQY_003373"
     CDS             complement(7733..9277)
                     /gene="malF"
                     /locus_tag="ACHOQY_003373"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9679418.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9431..10621)
                     /gene="malE"
                     /locus_tag="ACHOQY_003374"
     CDS             complement(9431..10621)
                     /gene="malE"
                     /locus_tag="ACHOQY_003374"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9679419.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10986..12101
                     /gene="malK"
                     /locus_tag="ACHOQY_003375"
     CDS             10986..12101
                     /gene="malK"
                     /locus_tag="ACHOQY_003375"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9679420.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12173..13513
                     /gene="lamB"
                     /locus_tag="ACHOQY_003376"
     CDS             12173..13513
                     /gene="lamB"
                     /locus_tag="ACHOQY_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9679421.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13756..14676
                     /gene="malM"
                     /locus_tag="ACHOQY_003377"
     CDS             13756..14676
                     /gene="malM"
                     /locus_tag="ACHOQY_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9679422.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14905..>15019
                     /locus_tag="ACHOQY_003378"
     CDS             14905..>15019
                     /locus_tag="ACHOQY_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679423.1"
                     /translation="MISHILNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVP"
BASE COUNT         3721 a         3861 c         3954 g         3483 t
ORIGIN      
        1 atcgctaatt ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg
       61 aaatgttttt gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc
      121 tgtatggcat ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac
      181 aggtgggcga agcggcaggg tcggcagcaa cgtcggtttc ggcggggagt tcctccgcta
      241 ccagcgtcag caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg
      301 atggttctaa taccgggacc accacaacca cgaccaccag tacccagtaa taaagtatgt
      361 atccccaaaa taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata
      421 accacactcc ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact
      481 cattcttatc tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc
      541 actgtgggac agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa
      601 tatgccctac gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct
      661 ggccttcgct gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac
      721 acaacatggt cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa
      781 ccttgccgcc gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg
      841 cacgatgggc tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa
      901 atgggatggc accgataccg tcaaagtcgg cagcgatgaa accctggttc gcgtgctgga
      961 cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg
     1021 gcaaattcgc cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct
     1081 catcaaggcg gcaaaacaat gattaaacaa acttttgtcg cgttgctttt gagtgtggga
     1141 gcgtcatcgg tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc
     1201 aaaacactaa caggcgcaga gcatttaatc gatctggtag gccaaccgcg gctggcaaac
     1261 agctggtggc ccggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag
     1321 cagcaggcgt tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc
     1381 gccgccatta acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa
     1441 atcaatcttg atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc
     1501 aactacacgc tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc
     1561 catcctggca atcagccatt cactccaggt cgcgacgtgg cgagctatct ctccgggcag
     1621 aacctgctta gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg
     1681 caaaaagcgc cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt
     1741 atttatgttg gcctcgcgga ctccgtctgg agtgagacgc ctgatgccct taacgccgac
     1801 attcttcaga ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct
     1861 tactggcgct gggcattagc atggcttgct acggcgaaac atatcctgcg cccattggcc
     1921 cgtcgcagtc ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac
     1981 gggaagggga gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt
     2041 cagtgcaact cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc
     2101 agtacagcag cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc
     2161 tcaaactgcg tttgtgggaa gagagttatt ggctgccgca agtggcggtt ggtgcgcggg
     2221 atatcggcgg tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc
     2281 cgttcgattt tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtgaaaa
     2341 atccgctctg ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg
     2401 gatctatcga cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat
     2461 accagacgcc ctggcaaccg ctgcgcctga aactggagta tgaaggcaat aattatcagc
     2521 aggattttgc cgggaagctg gagcaaaaaa gtaagtttaa cgtcggtgcg atttatcgcg
     2581 ttaccgattg ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg
     2641 ttacgttgcg caccaacttt aacgatctgc gcccgtctta caacgataac gcccgcccgc
     2701 aatatcaacc gcagccgcag gatgccattt tgcagcattc ggtggtggcg aatcagttaa
     2761 cgttgctgaa atacaatgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc
     2821 tgtatgttac cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcga
     2881 atcggatcgt gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acagaaaatc
     2941 gccttaacat gccgcaggtg acgacggaaa ccgatgtcgc cagcttgaaa aatcatctcg
     3001 ccggggagcc gctgggccat gaaacgaagg tggtgcaaaa acgcgtcgag ccagtggttc
     3061 cgaagtccac cgagcaggga tggtatatcg acaaatcacg ctttgatttc catatcgatc
     3121 cggtgctgaa ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga
     3181 tgggaacggc agatttgtgg ctgacggacc atctgctgac caccggcagc ctgtttgcaa
     3241 atcttgccaa caactacgac aagtttaact acaccaatcc gccgcaggac tcgcacttac
     3301 cgcgcgttcg tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc
     3361 aagccaacta cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc
     3421 tcgaaaccat gtttggcggt gccggggcag aagtgttgta tcgtccgctg gacagcaact
     3481 gggcgtttgg tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata
     3541 tgatgaaatt caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat
     3601 ctttcgccca ggacgtgttg gtgaaagcga gcgtcgggca gtatctggca ggggataaag
     3661 gcggcacgct ggagatcgcc aaacgctttg atagcggcgt ggtggttggc ggctatgcca
     3721 cgatcacgaa tgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg
     3781 tctctgtgcc gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct
     3841 ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca
     3901 tgaccagcga ccgtagcgtc aatttccgct aagtcatggg aaaggtgcca gttttcgcaa
     3961 tcactggcac cctcattctt aatggcctgt tgagccatca ggaacgcagt tcacccccgg
     4021 tttgcgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg
     4081 cagatctggt tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag
     4141 gtcggcttta tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa
     4201 catcaaagca gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg
     4261 ttccagagga acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg
     4321 gtgatttttg aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg
     4381 gcttatccgg cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg
     4441 ttgaacaaaa catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag
     4501 ctcgccactg tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta
     4561 tctccaccat tttgcagacc gtactcaatc ttggcctgtt gtgcctcggc ctgattttgg
     4621 ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa
     4681 ccagcaaata tgagctggta gaagggctgg tggtttactt tctctatttc gaatttatcg
     4741 cgctgattgt gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata
     4801 ttgggatcac cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtat
     4861 tgatctactc ggcagcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac
     4921 ggctgaagcg ggagtaaaaa gacagcacgc cgaactggcg cggcgtgctg gacaggaaga
     4981 ttacagtgta gcagtttgtt gtgttttctt cgtttccggt tcccagaggg cttccagctc
     5041 ctcaagggtt ttacctttgg tttccgggac aaatttccac ataaatagtg ctgccagaac
     5101 gcccatacaa ccgtaaatcc agtaggagaa accgttgtgg aaatgggtca ccagccagga
     5161 gtttttgtcc atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc
     5221 caccgcgatt gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac
     5281 ccagcatacc ggaccccagg acatggcaaa ggcagcaaca tagaacagca tcgacagtag
     5341 cgccacaata cccggtgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc
     5401 cattccgagc gcgccgataa tttgcagtgg cttacgacca aatttatcca ccgtcataat
     5461 tgccagaacg gtgaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat
     5521 atccgtgctg gcccccagcg ttttgaacac ttccggcgcg tagtacaaca ccacattgat
     5581 gccgacaaat tgctggaaga tggagagcat aacgccgata acaatcacgc ccacgccgaa
     5641 catcagcaga cgaccaccgg ttttgcgtcc atgatccagg gagtgtttaa tttcctgcac
     5701 cgcctgggct gccaaagcgt tgcccataat tttgcgcagg atgccttccg cttgttcttg
     5761 cttaccgcgt gacatcagcc agcgagggct ttccggcacg gtataaagca gcattaagaa
     5821 cagcagcgca gggatacatt ccgaagcaaa catataacgc cagccgtcag tattcagcca
     5881 ggtggcatcg ccggaacggg caataaaata gtttacgcag taaactaaaa gttggccgaa
     5941 aataatcgca aactggttaa aagagaccag cttcccacga atatgtgcag gagccagttc
     6001 cgcaatatac attggtgaga gcattgaggc taaaccaaca ccaataccgc caataatgcg
     6061 ataaataaca aattccggga cataacctgc cagataaaca ggcactgtgt tgtccgggtt
     6121 tatagaggta aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac
     6181 agcagcaatc ttaagtgaat cacgacgacc gaagcggtta ctgcaataac caccgagggc
     6241 accgccgatg atgcaaccaa tcagagcgct ggctacgcaa aaccctaaca gggagttggc
     6301 agcggattca cttaagtttt gtggagcaac aaagacggta tggagtgact caacagtacc
     6361 ggaaataacg gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat
     6421 cgaaaatata taactggaat tatactgggt attcattcag acctgcctta gaccattctg
     6481 atcttttatc agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt
     6541 acaatgcgtt tacgtaatgt cttttctgtg atcttaattg tgataattat ctaaaattga
     6601 tgaaaacaat actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa
     6661 caaataacgt aaaaataata agctctatga tgggaatatt aaccggcgaa cgattcagat
     6721 tgcagacgaa agaaaaaaag gcgctccgtg gagcgccgaa taacagtcac aagttgggat
     6781 aacgtaagtt gagggtgcag cggcataaca ttggcagaac aacatcttta acctttcaca
     6841 ccacctgccg tcaggccgtt gaccagccag cgttgagcca gcaagaagac gatggtgatc
     6901 ggtaatgcag acatcacggc agcggcggca aagtcacccc acaggtagtt ttgcgggttg
     6961 aggtattgct gcatccccac ggccagggtg tagctgttta cgtcacgcag taacagcgac
     7021 gcgaccggaa cttcagtaat ggcagcgata aacgacaaga tgaataccac cgccagaatc
     7081 ggtactgaca gcggcaacag gacaaggcgg aaggcctgcc acggtgtcgc accatccagc
     7141 gcagccgctt cttccagcga actatcgatg gtttcgaaat agcctttgat ggtccagaca
     7201 tgcagcgcaa tcccacccag atacgcgaaa attacgccgc cgtgagtatt caggccaatg
     7261 aatggaatgt actcacccag acgatcaaac aacgcataca acgcgaccag tgaaagtact
     7321 gccgggaaca tctggaaaat cagcattcct ttcagcagcg tcgctttgcc tggaaagcgc
     7381 atacgggcga aagcgtaggc gcaggtggta gagagcgcca caatgccaat cgcggaaatc
     7441 ccggcgacct ttaccgagtt ccacagccac agcagtaccg ggaatggcgg tggcgtaatg
     7501 cgaccatcag cctgttcaac gctaaaacct aacgccagtt tccagtgatc ccaggagatt
     7561 tgctccggga tcaggctacc ggtcgcgaag tttccctgac gcagcgagat agcgacgacc
     7621 atcagcagcg ggaacataat cgccgcgata aaaagtagca gtagcaggtg agtaataaac
     7681 aaacgtgctt tttgcgattt cggttggacc attgccattt ttgttatctc ccttaatcaa
     7741 acttcattcg cgtggctttc aggttcacta tcgccagcgc acccaccagc aggaagatca
     7801 gcgtggcaat tgctgctgcc agaccgaagt cctgaccccc gccgccttca aaagcgatgc
     7861 ggtaggtgta gttaacaagc aggtcggtat aaccggctgg cgtggtcgtg ccaagacgat
     7921 ccgggccgcc gttggttaac agttgaatca gcacgaagtt gttaaagtta aaggcgaagc
     7981 tggcgatcat cagcggcgtc agcggtttaa tcagcagcgg cagcgtaatc ttaaagaagt
     8041 tctggaacgg acctgcgcca tccattgctg aggcttcata caaatcgtcc ggaatcgctt
     8101 tcagcaagcc catgcagagg atcatcatgt acggataacc cagccaggta ttgacgataa
     8161 tcagcatcgt gcgggcggtg gtcggatcgc tgaaccaggc gggcttcacg ccaaacagcg
     8221 cgctcaacat catgttgatt tcaccgaagc tctggttaaa caaccctttg aaaatcaaga
     8281 ttgaaatgaa cgatggcacc gcgtagggca gaatcagcag gacgcgatag accgctttgc
     8341 cgcgcaacgc ttcccactgc accagacacg ccagaaccat gccgaccgcc accgttaaaa
     8401 agacagtgat cagcgagaac accacggtcc agacgaaaat ggcgaggaac ggtttctgaa
     8461 tgccttcgtc ggtgaagacg cgggtaaagt ttttccagcc agtagtcacg gtgtaaccgg
     8521 ggcttagctt ttcatcgccc cagttgccgt cggcggtaat ggactggtaa aagccaattt
     8581 ggttattcgg acgatatttc acgccgctct gattattcgt caacgtgccg tcaccgtcga
     8641 gtgtgtagag cggctgcgtg ccagaaaact ggcgcaggga gctcatcatc actttgttgc
     8701 catccggcag aatggcggta atgtcactca gcgcctgacg attctgggta atcacgcgca
     8761 gattcgcgcg ttcgccttcg ggctgggtgg tcgtttcttt cagttgcagt ttttgctcgc
     8821 cgccaaattt aaaagcgtcg gagaggtaat ttttgccggt ttcgccgtcg ctgagggcta
     8881 gttgccactc atcgcccgcc gggtaaagac caaaattata ggttttgcct gcttgccagg
     8941 agcgatctag caacacttcc tgcgcacgtt caaaagtcag ctggttagtg ctgctgtagt
     9001 tggtgaaggc aatggcgatg gtgcagacca gagggaagag gacgaataat cccattccag
     9061 ccattcccgg gtaaacatag cgccaggcgt aggctttacg attggcgaaa atatacagcc
     9121 ccgctgaact caatatcagc gtggtaatgg cgaacaggta ttccccttgt gcgtacatta
     9181 aaacaacaag gtaacccacc agcaggccga gcagacctaa cactgaccat ttcagcgcgt
     9241 cgctttgcca ccaatgtttc tttttaatga catccatggg gttcttcctc attccaggac
     9301 ggataaggct ttcacgcctt atccgacaac aactgcctga tgcgacgctg acgcgtctta
     9361 tcaggcctac atacgtttcg gttttgtagg ccggacaagg cgttcacgcc gcatccggca
     9421 tttcacagca ttacttggtg atacgagtct gcgcgtcttt cagggcttca tcgacagtct
     9481 gacgaccgct ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct
     9541 gcgggatgtt cggcatgatt tcacctttct gggcgttttc catggtggct gcaatacgtg
     9601 gatctttcgc caactcttcc tcgtaagact tcagcgctac ggcacccagc ggtttgtctt
     9661 tattaaccgc ttccagacct tcgtcagtca gcagatagtt ttcgaggaac tctttcgcca
     9721 gctctttgtt cggactggcg gcgttaatac cggcgctcag cacgccaacg aacggtttag
     9781 atggttgacc cttgaaggtc ggcagtaccg ttacaccata attcactttg ctggtgtcga
     9841 tgttggacca tgcccacggg ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag
     9901 cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt gtttttaatc aggtcaacca
     9961 ggaaggtcag acccgctttc gcgccagcgt tatccacgcc cacgtcttta atgtcgtatt
    10021 tgccattttc atacttgaac gcataacccc cgtcagcagc aatcagcggc caggtgaagt
    10081 acggttcttg caggttgaac atcagcgcgc tcttaccttt cgctttcagt tctttatcca
    10141 gcgccgggat ctcttcccag gtttttggtg ggttcggcag cagatctttg ttataaatca
    10201 gcgataacgc ttcaacagcg atcgggtaag caatcagctt gccgttgtaa cgtacggcat
    10261 cccaggtaaa cggatacagc ttgtcctgga acgctttgtc cggggtgatt tcagccaaca
    10321 ggccagattg agcgtagcca ccaaaacggt cgtgcgccca gaagataatg tcagggccat
    10381 cgccagttgc cgcaacctga gggaatttct cttccagttt atccggatgc tcaacggtga
    10441 ctttaattcc ggtatctttc tcgaatttct taccgacttc agcgagaccg ttatagcctt
    10501 tatcgccgtt aatccagatt accagtttac cttcttcgat tttggcgaga gccgaggcgg
    10561 aaaacatcat cgtcgttaat gcggataatg cgaggatgcg tgcacctgtt tttattttca
    10621 taatctatgg tccttgttgg tgaagtgctc gtgaaaacac ctaaacggac tctagtttct
    10681 ttatacggca acctctttcc atcctccttg cccctacgcc ccaccgttgc tttgtgtgat
    10741 ctctgttaca gaattggcgg taatgtggag atgcgcacat aaaatcgcca cgatttttgc
    10801 aagcaacatc acgaaattcc ttacatgacc tcggtttagt tcacagaagc cgtgttctca
    10861 tcctcccgcc tcctccccca taaaaaagcc agggggtgga ggatttaagc catctcctga
    10921 tgacgcatag tcagcccatc atgaatgttg ctgtcgatga caggttgtta caaagggaga
    10981 agggcatggc gagcgtacag ctgcaaaatg taacgaaagc ctggggcgag gtcgtggtat
    11041 cgaaagatat caatctcgat atccatgaag gtgaattcgt ggtgtttgtc ggaccgtctg
    11101 gctgcggtaa atcgacttta ctgcgcatga ttgccgggct tgagacgatc accagcggcg
    11161 acctgttcat cggtgagaaa cggatgaatg acactccgcc agcagaacgc ggcgttggta
    11221 tggtgtttca gtcttacgcg ctctatcccc acctgtcagt agcagaaaac atgtcatttg
    11281 gcctgaaact ggctggcgca aaaaaagagg tgattaacca acgcgttaac caggtggcgg
    11341 aagtgctgca actggcgcat ttgctggatc gcaaaccgaa agcgctctct ggcggtcagc
    11401 gtcagcgtgt ggcgattggc cgtacgctgg tggccgagcc aagcgtattt ttgctcgatg
    11461 aaccgctctc caacctcgat gctgcactgc gtgtgcaaat gcgtatcgaa atctcccgtc
    11521 tgcataaacg cctgggccgc acaatgattt acgtcaccca cgatcaggtc gaagcgatga
    11581 cgctggccga caaaatcgtg gtgctggacg caggtcgcgt ggcgcaggtt gggaaaccgc
    11641 tggagctgta ccactatccg gcagaccgtt ttgtcgccgg atttatcggt tcgccaaaga
    11701 tgaacttcct gccggtaaaa gtgaccgcca ccgcaatcga tcaagtgcag gtggagctgc
    11761 cgatgccaaa tcgtcagcaa gtctggctgc cagttgaaag ccgtgatgtc caggttggag
    11821 ccaatatgtc gctgggtatt cgcccggaac atctactgcc gagtgatatc gctgacgtca
    11881 tccttgaggg tgaagttcag gtcgtcgagc aactcggcaa cgaaacccaa atccatatcc
    11941 agatcccttc cattcgtcaa aacctggtgt accgccagaa cgacgtggtg ttggtagaag
    12001 aaggtgccac attcgctatc ggcctgccgc cagagcgttg ccatctgttc cgtgaggatg
    12061 gcactgcatg tcgtcgactg cataaggagc cgggcgttta agcaccccac aaaacacaca
    12121 aagcctgtca caggtgatgt gaaaaaagaa aagcaatgac tcaggagata gaatgatgat
    12181 tactctgcgc aaacttcctc tggcggttgc cgtcgcagcg ggcgtaatgt ctgctcaggc
    12241 aatggctgtt gatttccacg gctatgcacg ttccggtatt ggctggacag gtagcggcgg
    12301 tgaacaacag tgtttccaga ctaccggtgc tcaaagtaaa taccgtcttg gcaacgaatg
    12361 tgaaacttat gctgaattaa aattgggtca ggaagtgtgg aaagagggcg ataagagctt
    12421 ctatttcgac actaacgtgg cctattccgt cgcgcaacag aatgactggg aagctaccga
    12481 tccggccttc cgtgaagcaa acgtgcaggg taaaaacctg atcgaatggc tgccaggttc
    12541 caccatctgg gcaggtaagc gcttctacca acgtcatgac gttcatatga tcgacttcta
    12601 ctactgggat atttctggtc ctggtgccgg tctggaaaac atcgatgttg gcttcggtaa
    12661 actctctctg gcagcaaccc gctcctctga agctggtggt tcttcctctt ttgccagcaa
    12721 caatatttat gactatacca acgaaaccgc gaacgacgtt ttcgatgtgc gtttagcgca
    12781 gatggaaatc aacccgggcg gcacattaga actgggtgtc gactacggtc gtgccaacct
    12841 gcgtgataac tatcgtctgg ttgatggcgc atcgaaagac ggctggttat tcactgctga
    12901 acatactcag agtgtcctga agggctttaa caagtttgtt gttcagtacg ctactgactc
    12961 gatgacctcg cagggtaaag gtctgtcgca gggttctggc gtcgcgtttg ataacgaaaa
    13021 atttgcctac aatatcaaca acaacggtca catgctgcgt atcctcgacc acggtgcgat
    13081 ctccatgggc gacaactggg acatgatgta cgtgggtatg taccaggata tcaactggga
    13141 taacgacaac ggcaccaagt ggtggaccgt tggtattcgc ccgatgtaca agtggacgcc
    13201 aatcatgagc accgtgatgg aaatcggcta cgacaacgtc gaatcccagc gcaccggcga
    13261 caagaacaat cagtacaaaa ttaccctcgc acaacaatgg caggctggcg acagcatctg
    13321 gtcacgcccg gctattcgtg tcttcgcaac ctacgccaag tgggatgaga aatggggtta
    13381 tgactacaac ggcgatagca aggttaaccc gaactacggc aaagccgttc ctgctgattt
    13441 caacggcggc agcttcggtc gtggcgacag cgacgagtgg accttcggtg cccagatgga
    13501 aatctggtgg taatagcaaa acctgggccg gataaggcgt ttacgccgca ttcggcaacc
    13561 aacgcctgat gcgacgcttg cgcgtcttat caggccgaca acggctgtca aatgtaggcc
    13621 ggataaggcg tttacgccgc atccggcata aaaacaggtt gtcattatct gaaaggggcg
    13681 aaagcccctc tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat
    13741 tttgaggtga aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg
    13801 ttactggcaa gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc
    13861 agcgacgcgc cagccattcc atctgctgcg ctgcaacaac tcacctggac accggtcgat
    13921 caatctaaaa cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc
    13981 atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg
    14041 ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat
    14101 cagaacatga ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc
    14161 gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa
    14221 aaactttatg ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc
    14281 gacccggcta aagcctatgc caagggcgtc ggtaactcga tcccggatat ccccgatccg
    14341 gttgctcgtc ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc
    14401 agcgtgttgg taggaccttt atttggttct tccgctccag ctccggttac ggtaggtaac
    14461 acggcggcac cagctgtggc tgcacccgct ccggcaccgg tgaagaaaag cgagccgatg
    14521 ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat
    14581 gttgataagg cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt
    14641 tccaccttta tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg
    14701 caacaactgg tgcgtctcct ggcgcacctt tttttatgct tccttcctgg gatatgagcg
    14761 attttttata gtaactcact tcttcttcac taagaatatc cattatctca atgccttatc
    14821 agagattctt ttcctttcgc cggtagtgtc tggacattca ggctactttt ccaggttatt
    14881 ttatttctgt tatgcagagg ttttatgata agtcatatcc taaattctgg cggcaataac
    14941 tctttgatga aacatgatgt ggtgcaagga aataatatag tagatcttga tttactacgt
    15001 aatttcaatg gggtgccag
//
LOCUS       DAZKGS010000112        14981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKGS010000112 DAZKGS010000000
VERSION     DAZKGS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(71..146)
                     /locus_tag="ACHOQY_003379"
     tRNA            complement(71..146)
                     /locus_tag="ACHOQY_003379"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Phe,seq:gaa)
     gene            complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHOQY_003380"
     CDS             complement(252..959)
                     /gene="yqgA"
                     /locus_tag="ACHOQY_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9679424.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHOQY_003381"
     CDS             1112..1195
                     /gene="yqgH"
                     /locus_tag="ACHOQY_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFP9679425.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            1357..3492
                     /gene="speC"
                     /locus_tag="ACHOQY_003382"
     CDS             1357..3492
                     /gene="speC"
                     /locus_tag="ACHOQY_003382"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9679426.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGGNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHOQY_003383"
     CDS             complement(3542..4798)
                     /gene="nupG"
                     /locus_tag="ACHOQY_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9679427.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHOQY_003384"
     CDS             complement(5000..6079)
                     /gene="mltC"
                     /locus_tag="ACHOQY_003384"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9679428.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHOQY_003385"
     CDS             complement(6144..6419)
                     /gene="yggX"
                     /locus_tag="ACHOQY_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9679429.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHOQY_003386"
     CDS             complement(6447..7499)
                     /gene="mutY"
                     /locus_tag="ACHOQY_003386"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9679430.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7660..8379
                     /gene="trmB"
                     /locus_tag="ACHOQY_003387"
     CDS             7660..8379
                     /gene="trmB"
                     /locus_tag="ACHOQY_003387"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9679431.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8379..8705
                     /gene="yggL"
                     /locus_tag="ACHOQY_003388"
     CDS             8379..8705
                     /gene="yggL"
                     /locus_tag="ACHOQY_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9679432.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8889..9608
                     /gene="yggN"
                     /locus_tag="ACHOQY_003389"
     CDS             8889..9608
                     /gene="yggN"
                     /locus_tag="ACHOQY_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9679433.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9784..10830
                     /gene="ansB"
                     /locus_tag="ACHOQY_003390"
     CDS             9784..10830
                     /gene="ansB"
                     /locus_tag="ACHOQY_003390"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9679434.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10947..11954
                     /gene="yggM"
                     /locus_tag="ACHOQY_003391"
     CDS             10947..11954
                     /gene="yggM"
                     /locus_tag="ACHOQY_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9679435.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(12010..13311)
                     /locus_tag="ACHOQY_003392"
     CDS             complement(12010..13311)
                     /locus_tag="ACHOQY_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9679436.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13311..13814)
                     /locus_tag="ACHOQY_003393"
     CDS             complement(13311..13814)
                     /locus_tag="ACHOQY_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9679437.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13859..14845)
                     /locus_tag="ACHOQY_003394"
     CDS             complement(13859..14845)
                     /locus_tag="ACHOQY_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9679438.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3977 a         3587 c         3783 g         3634 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatc gaaccaagga cacggggatt ttcaatcccc
      121 tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc gcctcgtctc
      181 gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact ttgccgctat
      241 tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat tgccagaagg
      301 agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg taatccggtt
      361 gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg tgtggtcagc
      421 ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa tgggatacta
      481 attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga
      541 aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat cgccccgaag
      601 atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg aataaaagat
      661 tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc gactgctgta
      721 ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag cgtagctaaa
      781 accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc cagcgatgcc
      841 agaccaaaaa tagatgtcat ggagactcgg atacgttccg gtaagcgttg gctgagcagt
      901 gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc gatgaccaca
      961 agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc cttgttgatg
     1021 gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg
     1081 aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac attctctcgc
     1141 ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc ggtgaagtta
     1201 acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg
     1261 gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt tccaccttgt
     1321 ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa tattgccgcc
     1381 agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt gggagatact
     1441 gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag tggcattctt
     1501 gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga acatgctgtt
     1561 gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg gctggagctg
     1621 gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga cacgctgacg
     1681 cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca tggtgcgttt
     1741 tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa cgtctttcgc
     1801 gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga aggatcggcg
     1861 aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta ttttgtgctg
     1921 aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg
     1981 gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat tcaggcgggg
     2041 gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg cggtattgat
     2101 gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa
     2161 gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta tgacggaact
     2221 gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta cattctgttt
     2281 gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag ctcgccgctg
     2341 ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt gcacaaacag
     2401 caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat ccgcggacag
     2461 gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc ttctaccagc
     2521 cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga aggggagagt
     2581 gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt
     2641 gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa attgtggcag
     2701 gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga gccgggggcg
     2761 aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg
     2821 ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt tggcgttccg
     2881 gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa gtgcgatctc
     2941 aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc acaactggtg
     3001 gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc tgaggtgttg
     3061 ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt
     3121 caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc
     3181 cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag cgcttatatt
     3241 cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg
     3301 gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg gggtggggcg
     3361 gttcaacgtt atttccttgc actggaagaa ggggggaatt tgttgccggg attttcgccg
     3421 gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt gtacggttat
     3481 gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt tctttgcgta
     3541 attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat ttgaacatcg
     3601 ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat acggtctgcc
     3661 agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg atgccgccga
     3721 ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg gcgcgaattg
     3781 ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag tcgaatgcgc
     3841 aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc gggtcgccgt
     3901 aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt acgttcttaa
     3961 taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca gagatctgcg
     4021 aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac atcggatctt
     4081 tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt tccgcgccca
     4141 gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc gcgaatgcat
     4201 cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc ggaatatgcg
     4261 gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata tacagctgca
     4321 tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata aagccgatgg
     4381 tgccccagat acggattggc gggaagtcag taacgatatc catcccggca ttttgcaggc
     4441 gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac gagttaatca
     4501 atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac agcgtgatag
     4561 cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg tcggccacaa
     4621 tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa actgcgccaa
     4681 tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc gtcagccaac
     4741 ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta agattcatgt
     4801 taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga ccgtgcgaga
     4861 tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa ataattgcaa
     4921 acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc ctgagcatcg
     4981 tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta ttcactttat
     5041 aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga taaacatcgc
     5101 ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa aagactcgca
     5161 gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc gatgttgggt
     5221 tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg ccggtatcaa
     5281 tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc ccctgcgagc
     5341 ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc gcatcggaac
     5401 ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga atcagcgact
     5461 catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg tgcgcacgtt
     5521 tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc agcccgttgc
     5581 tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg cgaccttccc
     5641 agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt tctttcgaaa
     5701 tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca cccatcagca
     5761 acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg atagtttcga
     5821 tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga tcggtgtatt
     5881 tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg ttctcaatat
     5941 tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc caggcttcgt
     6001 tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca atcaaagcca
     6061 gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat gcaagttgcc
     6121 ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc ctcgatatgc
     6181 acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt gcggtgctcg
     6241 gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg ctgccactgc
     6301 gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc ggggtacagc
     6361 tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt tctgctcata
     6421 aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa ctgctgtaac
     6481 aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt ataccagagc
     6541 gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat aggcacaata
     6601 tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag gttatcggca
     6661 gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa ctgcgggaaa
     6721 cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac ttcatcttcg
     6781 tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg ttttttgccc
     6841 ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag cggacagagc
     6901 gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat cgcctgatta
     6961 aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa tttattctcg
     7021 acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac ccgtttgacg
     7081 ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc acctgcggtg
     7141 gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa tttaccgccg
     7201 tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc gtaatagcca
     7261 agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc ggtcaccgtc
     7321 gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg agtttgttgc
     7381 aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca gggcagcgtt
     7441 ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga cgcttgcatg
     7501 gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca gggtgtaaac
     7561 cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac tttggataat
     7621 gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga cgtcatttca
     7681 ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt gcgccgccag
     7741 gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt gatgggcgtt
     7801 gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc gccggtgacg
     7861 cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga tcgccctgag
     7921 caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct ggcttctgcg
     7981 catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga agtgctgcat
     8041 aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga cccgtggcac
     8101 aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact ggtaaaaagc
     8161 aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta tgcggaacat
     8221 atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag caatgattac
     8281 gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca tcgtcttggt
     8341 cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac cgtagccgtc
     8401 gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg gtggcatggc
     8461 gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat tttattaacg
     8521 aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc tgggaaggtc
     8581 tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt gtgcgtaagt
     8641 ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac gtttggtggg
     8701 actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta cgcttatcag
     8761 gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca tccggcatgg
     8821 aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt ttttcttgag
     8881 ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg gcaatgaccg
     8941 ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc agcccgcaaa
     9001 ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc aacgtgatgt
     9061 ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag gattatcagg
     9121 ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc gtcgaaaaag
     9181 cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc aaaatgcgca
     9241 gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt atcgaaacgc
     9301 gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc gaaggccagc
     9361 aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa atgggcgcga
     9421 aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc ctgggggggc
     9481 tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag cagtttggca
     9541 aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg gtcgggaatt
     9601 taaaataatc ctctatttta agacggcata atactttttt atgccgttta attcttcgtt
     9661 ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta aattgtttaa
     9721 cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa ctggaggaat
     9781 gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt tagtggtgca
     9841 gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc cggtggtggt
     9901 gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa tctggttaat
     9961 gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt gaatatcggc
    10021 tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa caccgactgc
    10081 gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga aaccgcttac
    10141 ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc aatgcgcccg
    10201 tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt ggccgcagct
    10261 gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt actggacggt
    10321 cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt taactacggt
    10381 cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc acgtaagcac
    10441 accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt cggcatcgtt
    10501 tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc gggctatgat
    10561 ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt cgacaccctg
    10621 gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc gacgggtgct
    10681 accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc tggcacgctg
    10741 aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa agatccgcag
    10801 cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt gccggggctt
    10861 tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa tcaccggaat
    10921 aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc acggcactgc
    10981 ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa aatatcttaa
    11041 aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc accttaaaaa
    11101 atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc tcttccagtg
    11161 acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa cagacctgga
    11221 cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg cccgcgtctg
    11281 gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg cgggtggtcg
    11341 acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc aattatcgct
    11401 ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg ctggaaaagg
    11461 aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat gcatattggg
    11521 gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa attcaccaac
    11581 agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa tatgacaaag
    11641 aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt tatgaagtgc
    11701 cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacagact tttctgcaat
    11761 cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa gggcaatatc
    11821 cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg atgaaaattg
    11881 acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg cggcgcaatg
    11941 gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag ttatccggca
    12001 aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt gcggaatgta
    12061 agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg gtttgattaa
    12121 atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac ccaccggcgg
    12181 tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta ctgggtccat
    12241 tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag ccggtgtcat
    12301 gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata tgatcagtgg
    12361 gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag cattagtcat
    12421 tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag tggtaacaac
    12481 cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa ccagcaccga
    12541 gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa aaatgccgcc
    12601 cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat ccagaaacgc
    12661 tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc gtttagcaat
    12721 aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat atcccgccat
    12781 aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta acacgtttga
    12841 gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag aaaatgccgg
    12901 atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag cagctgccgc
    12961 aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca catgaccaag
    13021 tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta tcgcaatacc
    13081 gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata gggcaaagtt
    13141 atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc agggaaacat
    13201 caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca caccaataaa
    13261 gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca ttaactggcc
    13321 tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa ggcataaaac
    13381 atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg tatttgcatc
    13441 gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc accagtcaca
    13501 atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag aagtaattgc
    13561 ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg cgcgccgacg
    13621 gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt
    13681 aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc
    13741 agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg
    13801 cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc ccggaatatt
    13861 attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt
    13921 cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac
    13981 cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt
    14041 gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct ggggttaatt
    14101 tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag
    14161 aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg
    14221 cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg
    14281 gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga acacgaattt
    14341 tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac
    14401 ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt
    14461 taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca
    14521 aattatagat agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca
    14581 tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc atctggctgc
    14641 tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct ttcgccagct
    14701 cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca
    14761 ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg
    14821 agcgggtaaa tgacctcatt ttcataaaaa aactctctgt tgaaggatgg aaattggttg
    14881 atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt ctgacaactt
    14941 gtctatgttt atcacaactt gaaataaagt cagaaattaa a
//
LOCUS       DAZKGS010000113        14806 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKGS010000113 DAZKGS010000000
VERSION     DAZKGS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..218)
                     /locus_tag="ACHOQY_003395"
     CDS             complement(<1..218)
                     /locus_tag="ACHOQY_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679439.1"
                     /translation="MSGKPAARQGDMTQYGSSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACHOQY_003396"
     CDS             complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACHOQY_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9679440.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            793..2991
                     /gene="priA"
                     /locus_tag="ACHOQY_003397"
     CDS             793..2991
                     /gene="priA"
                     /locus_tag="ACHOQY_003397"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9679441.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3147..4172
                     /gene="cytR"
                     /locus_tag="ACHOQY_003398"
     CDS             3147..4172
                     /gene="cytR"
                     /locus_tag="ACHOQY_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9679442.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4264..5223
                     /gene="ftsN"
                     /locus_tag="ACHOQY_003399"
     CDS             4264..5223
                     /gene="ftsN"
                     /locus_tag="ACHOQY_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9679443.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5316..5846
                     /gene="hslV"
                     /locus_tag="ACHOQY_003400"
     CDS             5316..5846
                     /gene="hslV"
                     /locus_tag="ACHOQY_003400"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9679444.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5856..7187
                     /gene="hslU"
                     /locus_tag="ACHOQY_003401"
     CDS             5856..7187
                     /gene="hslU"
                     /locus_tag="ACHOQY_003401"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9679445.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7254..8180
                     /gene="menA"
                     /locus_tag="ACHOQY_003402"
     CDS             7254..8180
                     /gene="menA"
                     /locus_tag="ACHOQY_003402"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9679446.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8273..8758
                     /gene="rraA"
                     /locus_tag="ACHOQY_003403"
     CDS             8273..8758
                     /gene="rraA"
                     /locus_tag="ACHOQY_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9679447.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACHOQY_003404"
     CDS             complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACHOQY_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9679448.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9513..10358
                     /gene="glpF"
                     /locus_tag="ACHOQY_003405"
     CDS             9513..10358
                     /gene="glpF"
                     /locus_tag="ACHOQY_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9679449.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10381..11889
                     /gene="glpK"
                     /locus_tag="ACHOQY_003406"
     CDS             10381..11889
                     /gene="glpK"
                     /locus_tag="ACHOQY_003406"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9679450.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12024..13034
                     /gene="glpX"
                     /locus_tag="ACHOQY_003407"
     CDS             12024..13034
                     /gene="glpX"
                     /locus_tag="ACHOQY_003407"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9679451.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13131..13877
                     /gene="fpr"
                     /locus_tag="ACHOQY_003408"
     CDS             13131..13877
                     /gene="fpr"
                     /locus_tag="ACHOQY_003408"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9679452.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13882..14310)
                     /gene="uspD"
                     /locus_tag="ACHOQY_003409"
     CDS             complement(13882..14310)
                     /gene="uspD"
                     /locus_tag="ACHOQY_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9679453.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14337..14636)
                     /locus_tag="ACHOQY_003410"
     CDS             complement(14337..14636)
                     /locus_tag="ACHOQY_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9679454.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
BASE COUNT         3562 a         3804 c         3992 g         3448 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcaggggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatgcg caccccggct gaaccctgga caatgctact gccatactgc
      181 gtcatgtcgc cctgacgcgc tgccggtttt ccgctcatcg taaaatcctt ttgaaataaa
      241 taagaaccag aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg
      301 aatttttcag aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt
      361 ttttcggaaa tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca
      421 acacggccac cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg
      481 cacacgtcga ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa
      541 gagcagctag cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct
      601 cggtttaagg ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt
      661 atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt
      721 atgcaaactg atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt
      781 caggatgatg ctatgcccgt agcccacgtt gccttgcccg ttccgcttcc tcgtaccttt
      841 gactatctgc tgccagaagg catgacggtt aaagctgggt gtcgcgtgcg cgtgccgttt
      901 ggcaaacagc aggagcgcat cgggattgtg gtatcagtta gcgatgccag cgaactgccg
      961 ctcaatgagc taaaagcggt agtcgaagtg ctggacgttg agccggtatt tactcactcc
     1021 gtctggcggt tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg
     1081 tttcatgcct tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg
     1141 tactggtttg ccactgaaca aggtcaggcg gtggatctta acagcctgaa acgttcgcca
     1201 aagcaacaac aggcgctggc ggcgttacgg caaggcaaaa tctggcgcga ccaggtcgcg
     1261 gaacttgaat ttaatgatgc cgcgttgcag gcgctgcgca aaaaaggtct gtgtgattta
     1321 gcaagtgaaa caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg
     1381 ttgcgattga ataccgaaca agccaccgcc gttggcgcaa ttcatagcgc ggcagatact
     1441 ttttctgcct ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt
     1501 gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc
     1561 ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta atgcccccgt ggaagttctg
     1621 cattccggcc tgaacgacag cgagcggctt tcggcgtggt tgaaagcgaa aaatggcgag
     1681 gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg
     1741 attgtcattg atgaagagca cgacagctcc tacaagcagc aggaaggctg gcgctatcat
     1801 gcccgcgacc tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc
     1861 gcaacgcccg cgctggaaac gttatgcaac gtccagcaga aaaaataccg cctgctgcgc
     1921 ctgacccgtc gggcagggaa tgcgcgtccg gcaattcaac atgtgctgga tttaaaaggt
     1981 cagaaggtgc aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag
     2041 gccaacaatc aggttatcct ctttcttaat cgtcggggtt ttgcgcctgc gctgctgtgc
     2101 cacgactgcg gctggatagc cgaatgccca cgttgcgatc actactacac gctgcatcag
     2161 gcgcaacagc atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc
     2221 ccttcctgcg gttccacgca cctggtcccc gtggggctgg gcaccgaaca gcttgaacag
     2281 acgcttgcgc cgatgttccc cggcgtgccc atttcacgta tcgaccgcga taccaccagc
     2341 cgcaaagggg cgctggaaca gcaactggcg gaagtgcatc gcggcggcgc gaggatcttg
     2401 attggcacgc aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta
     2461 ctggatgtgg acggcgcgct gttttctgcc gattttcgct cggcagaacg tttcgcccag
     2521 ctttacaccc aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg
     2581 caaacgcacc atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc
     2641 tttgccgaac aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat
     2701 gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg
     2761 cgtaatctga tcctttccag cccgctggca gacgacaaac tgtgggttct cggcccggtt
     2821 ccggctctgg cacctaagcg tggtggccgc tggcgctggc agatcttgtt gcagcaccct
     2881 tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg cgcttattaa tacaataccg
     2941 gattcccgca aggtgaaatg ggtgttggat gtcgatccca tcgaaggcta atgcgcaaac
     3001 gttgcgaggc ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc
     3061 gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg
     3121 cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca
     3181 tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga
     3241 atcccgataa agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg
     3301 gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg
     3361 tgattgtccc ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta
     3421 cggcggcaaa tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg
     3481 aaaaaacctt tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt
     3541 caaggctgcc gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg
     3601 cgaacgaatt cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg
     3661 catttgatgc agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg
     3721 gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc
     3781 gctgcggcat tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg
     3841 gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc
     3901 atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc
     3961 cggaagacct ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc
     4021 cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc
     4081 ttgatcaaat gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta
     4141 tcatccgggg atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg
     4201 tcaaaggccc gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg
     4261 atagtggcac aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag
     4321 agcacctcac ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt
     4381 gctgccgccg ttcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa
     4441 gaagagtccg agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca
     4501 gaagaacgct ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc
     4561 acagaacctt ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt
     4621 cagcttcttg aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt
     4681 ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg
     4741 cagcaattag cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg
     4801 cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa
     4861 ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg
     4921 cagtcgaagc cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg
     4981 gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa
     5041 caggcagaga cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc
     5101 aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc
     5161 accctcaatc ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt
     5221 tgaaaccctc aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg
     5281 tttgtactct gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc
     5341 gtaacggcca tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga
     5401 aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg
     5461 gcggtactgc ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc
     5521 agggccatct ggtcaaagcc gccgttgagc tagcgaaaga ctggcgtacc gatcgcatgc
     5581 tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg
     5641 gtaacggtga cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt
     5701 acgcccaggc tgcggcgcgt gcactgttag aaaatactga actgagtgct cgtgaaattg
     5761 ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca
     5821 tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa
     5881 tcgtcagcga actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga
     5941 ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga
     6001 ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc
     6061 gtctggctaa gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag
     6121 taggctacgt cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga
     6181 aaatggtacg cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag
     6241 aacgtattct cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc
     6301 agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg
     6361 atgacaaaga aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc
     6421 ctccaggcat ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc
     6481 agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag
     6541 aagcggcgaa actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc
     6601 agcacgggat cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg
     6661 gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca
     6721 ccgtttccac caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg
     6781 cgttccagat tgcgaaaccg tctgacctaa tcccggaact gcaaggtcgt ctgccaatcc
     6841 gcgttgaact gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg
     6901 cctctatcac cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta
     6961 ccgactccgg tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa
     7021 acatcggtgc tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg
     7081 acgccagcga tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc
     7141 tggatgcgtt ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca
     7201 ttttcatcat tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg
     7261 aacaacaaat tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc
     7321 tcgcctttgc tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc
     7381 cgctggtcgc cctgctggca ctaattaccg ccgggctatt acagatcctt tctaacctcg
     7441 ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac
     7501 gcggcatgca aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg
     7561 tcgtgctcat ctgtctctcc gggttggcac tggttgcagt ggcatgccat acgctggccg
     7621 attttgtcgg tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg
     7681 tgggcaatcg tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg
     7741 gctggttgag tgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactaa
     7801 tccttccggc gaccgcatgc ggcctgctgg caacggcagt actgaatatt aataacctgc
     7861 gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag
     7921 tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc gctggtgtgt ctggcgctgt
     7981 ttaatctctt ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac
     8041 tggtgaagca agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc
     8101 tggaacgtac tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc
     8161 taagccagtg ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag
     8221 cccacatagc gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata
     8281 cgatacttcc gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt
     8341 ctccaacttt ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga
     8401 ggacaacggg ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt
     8461 cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt
     8521 acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg cgtcaggtag atgacctgga
     8581 agagttggat atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg
     8641 cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc accttcttct ccggcgacca
     8701 tctttatgcc gacaataccg ggattattct ttctgaagat ccgctggata ttgaatgata
     8761 gaaaaggcac cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa
     8821 ttacgcggaa gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac
     8881 gttcctgcca gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc
     8941 gctgatgctg ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt
     9001 cgatttccat ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt
     9061 tctcaaacac ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata
     9121 aaaatcctcg tcccgattac cggtgacgcc ttaataaata cgagcgcact ttagttagct
     9181 ccgattgtat gaagccgcgc catcgctgtc cagcggcacg ccttgcagat tacggtttgc
     9241 cacacttttc atccttctcc tggtgacata atccacatca atcgaaaatg ttaataaatt
     9301 tgttgcgcga atgatctaac aaacatgcat catgtacaat cagatggaat aaatggcgcg
     9361 ataacgctca ttttatgacg aagcacacac attttaagtt cgatatttct cgcttttgct
     9421 cgttaacgat aagtttacag catgcctaca agcatcgtgg aggtccgtga ctttcacgca
     9481 tacaacaaac attaactctt caggatccga ttatgagtca aacatcaacc ttgaaaggcc
     9541 agtgcattgc tgaattcctc ggtaccgggt tgttgatttt cttcggtgtg ggttgcgttg
     9601 cagcactaaa agtcgctggt gcgtcttttg gtcagtggga aatcagtgtc atttggggac
     9661 tgggggtggc aatggccatc tacctgaccg caggggtttc cggcgcgcat cttaatcccg
     9721 ctgttaccat tgcattgtgg ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta
     9781 tcgtttcaca agttgccggc gctttctgtg ctgcggcttt agtttacggg ctttactaca
     9841 atttattttt cgacttcgag cagactcatc acattgttcg cggcagcgtt gaaagtgttg
     9901 atctggctgg cactttctct acttacccta atcctcatat caattttgtg caggctttcg
     9961 cagttgagat ggtgattacc gctattctga tggggctgat cctggcgtta acggatgatg
    10021 gcaacggtgt accacgcggc cctttggctc ccttgctgat tggtctactg attgcggtca
    10081 ttggcgcatc tatgggccca ttgacaggtt ttgccatgaa cccagcgcgt gacttcggtc
    10141 cgaaagtctt tgcctggctg gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca
    10201 ttccttactt cctggtgccg cttttcggcc ctatcgttgg cgcgattgta ggtgcatttg
    10261 cctaccgcaa actgattggt cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg
    10321 aaaccacaac tccttcagaa caaaaagctt cgctgtaata tgactacggg acaattaaac
    10381 atgactgaaa aaaaatatat cgttgcgctc gaccagggca ccaccagctc ccgcgcggtc
    10441 gtaatggatc acgatgccaa tatcattagc gtgtcgcagc gcgaatttga gcaaatctac
    10501 ccaaaaccag gttgggtaga acacgaccca atggaaatct gggccaccca aagctccacg
    10561 ctggtagaag tgctggcgaa agccgatatc agttccgatc aaattgcagc tatcggtatt
    10621 acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa ccggcaagcc tatctataac
    10681 gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg agcatttaaa acgtgacggt
    10741 ttagaagatt atatccgcag caataccggt ctggtgattg acccgtactt ttctggcacc
    10801 aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg agcgtgcacg tcgtggtgaa
    10861 ttgctgtttg gtacggttga tacgtggctt atctggaaaa tgactcaggg ccgtgtccat
    10921 gtgaccgatt acaccaacgc ctctcgtacc atgttgttca acatccatac cctggactgg
    10981 gacgacaaaa tgctggaagt gctggatatt ccgcgcgaga tgctgccaga agtgcgtcgt
    11041 tcttccgaag tatacggtca gactaacatt ggcggcaaag gcggcacgcg tattccaatc
    11101 tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc agttgtgcgt gaaagaaggg
    11161 atggcgaaga acacctatgg cactggctgc tttatgctga tgaacactgg cgagaaagcg
    11221 gtgaaatcag aaaacggcct gctgaccacc atcgcctgcg gcccgactgg cgaagtgaac
    11281 tatgcgttgg aaggtgcggt gtttatggca ggcgcatcca ttcagtggct gcgcgatgag
    11341 atgaagttga ttaacgacgc ctacgattcc gaatatttcg ccaccaaagt gcaaaacacc
    11401 aatggtgtgt atgtggttcc ggcatttacc gggctgggtg cgccgtactg ggacccgtat
    11461 gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg ctaaccacat tatacgcgcg
    11521 acgctggagt ctattgctta tcagacgcgt gacgtgctgg aagcgatgca ggccgactct
    11581 ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag tagcaaacaa tttcctgatg
    11641 cagttccagt ccgatattct cggcacccgc gttgagcgcc cggaagtgcg cgaagtcacc
    11701 gcattgggtg cggcctatct cgcaggcctg gcggttggct tctggcagaa cctcgacgag
    11761 ctgcaagaga aagcggtgat tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt
    11821 aattaccgtt acgcaggctg gaaaaaagcg gttaaacgcg cgatggcgtg ggaagaacac
    11881 gacgaataat gtaaatgccg aatgaagcgt ttatgccgca tccggtagtc ccgaaacgtg
    11941 cgggggcaac cccgcacaca tcaataatcc ctcccttccc ctgtgctaca cttcgcgcca
    12001 ttccttactg cttagagttt gctatgagac gagaacttgc catcgaattt tcccgcgtca
    12061 ccgaatcagc ggcgctggct ggctacaaat ggttaggacg cggcgataaa aacaccgcgg
    12121 acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca ggtcaacatt gacggcacca
    12181 tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct ctacattggt gaaaaagtcg
    12241 gtactggtcg cggcgacgcg gtagatattg ctgttgatcc gattgaaggc acgcgcatga
    12301 cggcgatggg ccaggctaac gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc
    12361 tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg gccgggagcc aaaggcacca
    12421 ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac
    12481 cgttgagcga actgacggta acgattctgg ctaaaccacg ccacgatgcc gttatcgctg
    12541 aaatgcagca actcggcgta cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa
    12601 ttctcacctg tatgccagac agcgaagttg acgtgctgta cggtattggt ggcgcgccgg
    12661 aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc
    12721 tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg tcgcattggc gagcaggagc
    12781 tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt attgcgcctg ggcgatatgg
    12841 cgcgcagcga taacgtcatc ttctctgcca ccggtattac caaaggcgat ctgctggaag
    12901 gcattagccg caaaggcaat atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac
    12961 gcaccattcg ccgcattcag tccatccact atctggatcg caaagacccg gaaatgcagg
    13021 tgcacatcct ctgattgatt tgatcgattg agccttccag tccttcggga ctggaatttt
    13081 tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt
    13141 gggtaacagg caaagtcact aaagtgcaga actggaccga cgccctgttt agtctcaccg
    13201 ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac caagcttggc cttgaaatcg
    13261 acggcgaacg cgtccagcgc gcctactcct atgttaactc gcccgataat cccgatctgg
    13321 agttttacct ggtcaccgtc cccgatggca aattaagccc acgattggcg gcactgaaac
    13381 caggcgatga agtgcaggtg gttagcgaag cagctggctt ctttgttctg gatgaagtac
    13441 cggactgcga aacgctatgg atgctggcaa ccggtacagc gattggccct tatttatcga
    13501 ttctgcaact aggcaaagat ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac
    13561 gttatgccgc cgacttaagc tatttgccac tgatgcaaga actggaaaaa cgctacgaag
    13621 ggaaactgcg cattcagacg gtggtcagtc gggaaacggc agcggggtcg ctcaccgggc
    13681 ggataccggc gttaattgaa agtggggaac tggaaagcgc gattggcctg ccgatgaata
    13741 aagaaaccag ccatgtgatg ctgtgcggca atccacagat ggtgcgcgat acacaacagt
    13801 tgctgaaaga gacccggcag atgacgaaac atttacgtcg ccgaccgggc catatgacag
    13861 cggagcatta ctggtaagcg gttacttatc gataaacggc acgatgagca aatccgcact
    13921 catcttattg atcatcccgc gatatgccgg catcaaacgg ttgataaatg agtgatgatg
    13981 accacagaca aggaggtcgc actgctcttt ttgcataatt tccagcagtg tttccggcat
    14041 ttctccgcgt tcaatacgca gttttgtctt cggccattga atatttttcg tcagtttata
    14101 cagcttgtta tccgacttat tcttcaacaa ttgaagaata tcttctgttg cagggaagta
    14161 gatacccgga tacaactcgc ttaagccatc atcaatatga attaacgtca ggtgagcgtc
    14221 attatgtctg gcgagctcca gagctttatt caccagtaag gcatcttctt cattcccgga
    14281 aattgccacg ccaatgtgtt tataagccat gtttaactcc ttctaaagca actccatcaa
    14341 gctaacgaac gcagtgataa ctcaaaaata atcatctcgg cctggcatga gaaagtgaag
    14401 gccgcatcaa gctcaacttg cccctccaga tccttaatct tgtaggccac gctggcccct
    14461 tcatctgcag aggatgcagc ggcggcaagc tctttcagtt tggtcataaa agactctgct
    14521 tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac agttgtcgtt atcaatcacc
    14581 gtaccgatat caatcgcaca tcctttagta ctgcatttat ctacgacatc tttcataggg
    14641 cacctctgta tattcgcctc tctgtcgatt acgacttaag aataatgcgc cgttaaggga
    14701 agaacaaaca agtaatgatc ttatggatta ccattttttt tgatattgct taaggtgaag
    14761 agttcagaag gattttccgg gtattgtcat ttgcatcgtg gaaata
//
LOCUS       DAZKGS010000114        14484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKGS010000114 DAZKGS010000000
VERSION     DAZKGS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..659
                     /locus_tag="ACHOQY_003411"
     CDS             93..659
                     /locus_tag="ACHOQY_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9679455.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            710..3169
                     /gene="ybgQ"
                     /locus_tag="ACHOQY_003412"
     CDS             710..3169
                     /gene="ybgQ"
                     /locus_tag="ACHOQY_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9679456.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3184..3915
                     /gene="ybgP"
                     /locus_tag="ACHOQY_003413"
     CDS             3184..3915
                     /gene="ybgP"
                     /locus_tag="ACHOQY_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9679457.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3912..4973
                     /gene="ybgO"
                     /locus_tag="ACHOQY_003414"
     CDS             3912..4973
                     /gene="ybgO"
                     /locus_tag="ACHOQY_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9679458.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5125..6171
                     /gene="abrB"
                     /locus_tag="ACHOQY_003415"
     CDS             5125..6171
                     /gene="abrB"
                     /locus_tag="ACHOQY_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9679459.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6168..6959)
                     /gene="nei"
                     /locus_tag="ACHOQY_003416"
     CDS             complement(6168..6959)
                     /gene="nei"
                     /locus_tag="ACHOQY_003416"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9679460.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6995..7729)
                     /gene="pxpA"
                     /locus_tag="ACHOQY_003417"
     CDS             complement(6995..7729)
                     /gene="pxpA"
                     /locus_tag="ACHOQY_003417"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9679461.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7719..8651)
                     /gene="pxpC"
                     /locus_tag="ACHOQY_003418"
     CDS             complement(7719..8651)
                     /gene="pxpC"
                     /locus_tag="ACHOQY_003418"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9679462.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSLQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8645..9301)
                     /gene="pxpB"
                     /locus_tag="ACHOQY_003419"
     CDS             complement(8645..9301)
                     /gene="pxpB"
                     /locus_tag="ACHOQY_003419"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9679463.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9324..10067)
                     /gene="ybgI"
                     /locus_tag="ACHOQY_003420"
     CDS             complement(9324..10067)
                     /gene="ybgI"
                     /locus_tag="ACHOQY_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9679464.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10338..11819
                     /gene="dtpD"
                     /locus_tag="ACHOQY_003421"
     CDS             10338..11819
                     /gene="dtpD"
                     /locus_tag="ACHOQY_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9679465.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11861..13279)
                     /gene="phrB"
                     /locus_tag="ACHOQY_003422"
     CDS             complement(11861..13279)
                     /gene="phrB"
                     /locus_tag="ACHOQY_003422"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9679466.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13276..13785)
                     /gene="ybgA"
                     /locus_tag="ACHOQY_003423"
     CDS             complement(13276..13785)
                     /gene="ybgA"
                     /locus_tag="ACHOQY_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9679467.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13936..14484)
                     /locus_tag="ACHOQY_003424"
                     /pseudo
     CDS             complement(13936..14484)
                     /locus_tag="ACHOQY_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3496 a         3864 c         3510 g         3614 t
ORIGIN      
        1 aatattttta agaaaaacta aaaacattcg acaatcaaat aagatattct tgcaccgata
       61 tacttttttc ttttctgtta aaggactccg ttatgtttaa aggacaaaaa acattggccg
      121 cactggccgt atctctgctg ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg
      181 aaattcactt taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg
      241 ataaagaggt tgaactcggt caggtgacca ccagccacat taatcagtca catcacagcg
      301 atgccgttgc tgtcgacctg cgcttagtca actgtgatct ggaaaactcc agcaacggtt
      361 ccggtggcaa gatttctaaa gttgcagtca cctttgatag ctcagcgaaa accaccggcg
      421 cagatccgat tctcaacaac accagcacgg gtgaagccac cggcgtcggc gtacgtttaa
      481 tgaataaaga tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggctc
      541 cgacctccag cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt gatcaggcta
      601 cacctgtaac gccaggcgca gttacggcga atgcaaccta cgtgctggat tataaataag
      661 tattactttg gcggcgctat cgccgccttt cataaaatat ttatcaggta tggacacagt
      721 gaatatttat cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc
      781 ggtcgaattc aaccttaatg ttctcgataa atcaatgcgc gaccgcattg atatttcatt
      841 attaaaggaa aaaggagtca ttgctcccgg tgagtatttt gttagcgttg cggtaaataa
      901 taaccaaatc agtaacgggc aaaagattaa ctggcacaaa aatgacgata aaaccattcc
      961 gtgcatcaat gatttactgg tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt
     1021 accattgata aatcagtgcg tcgattttag ctcccgacct gaaatgctct tcaatttcga
     1081 tcaagccaat cagcaactaa atatcaccat tccgcaagcc tggctggcgt ggcactcaga
     1141 aaactggacc ccaccctcca catggaaaga aggtgtcgcc ggtgtcctga tggattacaa
     1201 cttgtttgcc agcagctacc gcccacagga tggcagcagc agcactaatc ttaacgccta
     1261 tggaacagcc ggaatcaaca cgggggcatg gcgcttacgc agtgattatc agttgaatca
     1321 gactgatagc gatgataacc atgaacagtc aggcgaaata tcacgtacct atctttttcg
     1381 cccgttaccg caattaggtt caaaattaac cctcggcgaa acggatttta gttccaatat
     1441 tttcgacggt ttttcttata ccggcgcggc actggcaagt gacgatcgaa tgttgccatg
     1501 ggaactacgc ggctacgccc cacaaattag cggtattgca cagaccaatg ccacggtgac
     1561 gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc catttatcat
     1621 tgacgacctt aatcagtctg ttcagggcac actggatgtc aaagtgacgg aagaagatgg
     1681 tcgggtgaac aatttccagg tttcggcagc atcgacgccc ttcctgactc gtcagggaca
     1741 ggttcgctat aaactggccg cgggtcagcc acggccctcc atgtcacatc aaactgaaaa
     1801 tgaaaccttt tttagcaatg atgtttcctg ggggatgctg tcaaacacct cgctgtacgg
     1861 cggcctgctg ctttctggtg atgactacca ttctgccgca ataggtatcg ggcaaaatat
     1921 gctgtggctt ggcgcgctgt cgtttgatgt cacgtgggcc agtagtcact ttgatactca
     1981 gcaggacgag cggggcttaa gctaccgttt taattacagc aaacaagtgg atgccaccaa
     2041 cagcacgatt tccctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa
     2101 ctatcttgat cacaaataca acgacagcga tgcgcaggac gaaaaacaga cgatcagctt
     2161 atctgtgggc caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca
     2221 aacctggtgg aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga
     2281 tattggtgac tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga
     2341 taaagatcgc gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg
     2401 ggttggctat gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga
     2461 tacgctggat gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtccgga
     2521 caatggagcc caggtgagcg gtaactatca gcatctgagt tcagcgggtg agtgggatat
     2581 ttctggtacc tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt
     2641 caccgcaacc caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct
     2701 gatggtcagc accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa
     2761 ccattttggc attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt
     2821 gaacatgaat gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg
     2881 gattgaaggc gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt
     2941 catcattcgc aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga
     3001 cagcggcatt agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc
     3061 tgaaaatcaa aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc
     3121 tgaacatatg gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg
     3181 aaaatgacat ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac
     3241 gctgcactac aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt
     3301 ctgcgggtgg ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat
     3361 gaaaaagggg agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa
     3421 ccgaaagcaa caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc
     3481 gatcgcgaaa cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac
     3541 agcgaccatg ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg
     3601 gctgcattac gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag
     3661 ggcaaccaac tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga
     3721 cgaaatgaaa aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg
     3781 gtcaacacaa acatcggaaa ttatagcggc agccagtttt atctcggtta tatggatgat
     3841 tacggcgcgt tacgcatgac gacgctcaac tacagcggac aatgccattt acaagccgtg
     3901 gaggcgaaga aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc
     3961 cgttgaagtc tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa
     4021 aatcagaagc gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt
     4081 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg
     4141 aaagtgagca tgtttatgcc tgggtaaacc cctacccggg ggtgcaggat cgctattatc
     4201 aactcggcgt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
     4261 acactaacca gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg
     4321 cgatgggatg gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcacgta
     4381 cctttctcgc tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc
     4441 aaagtacgct tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag
     4501 atcctactgc ccaaaacctg aaatatcata ttaccggtct ggaaaacatt cgcgtgctgg
     4561 attgcagcgt caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta
     4621 atttgctgga tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat
     4681 cacaaaatga ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa
     4741 cgttggttga agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg
     4801 atgaaaacgc ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg
     4861 acatgttggt ctacgaaaaa acctatacgg ctgaactttc gtccattgca ggtaccccca
     4921 tcgaggctgg gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca
     4981 tgatgccctt tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt
     5041 ccttgattgc tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa
     5101 gagaaaataa tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta
     5161 tcacttctcc tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg
     5221 ccgatgatcg ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc
     5281 gcttttctcg ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg
     5341 attctcacca cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg
     5401 ctttccagcg ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc
     5461 ggtgcctggg gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac
     5521 ggcgcagata ttcgcctggt ggcgtttatg caatatctgc gagtgctgtt cgtcgcgggg
     5581 gccgctgttc tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa
     5641 atcgtctggt tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt
     5701 gccggtacgg cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg
     5761 gcgggcgcag tgctccagtc tggtcagctc atcaccatcg aactcccgga atggctgctt
     5821 gcgatggcgt atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta
     5881 ctgcgggcac tacgcccgct accgcaaatc ctgctgtcga tttttgctct gttggctatt
     5941 tgtgcgggta tggcgtgggg gctgacccgg tttatgcata ttgattttat gactgcctac
     6001 ctcgccacca gccccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc
     6061 gatatggcgc ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacggga
     6121 cctgccattg cacggtttat ttcaacctat gcgccgaagc ggtcggctta gtgctggcag
     6181 ccagggcacc agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gctgccacaa
     6241 cgttcgcacg gttcgccatc tcgatgaaaa accttaaagc gaaacagcgc cccatgatgc
     6301 ttattctcat ccacctgccc ccgcgtagcg taagaaaatc gaggaatctc cagtaacgcg
     6361 tgtgcgagtg catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac
     6421 ccaacctgcc agaggatctc cacccgcaaa taattgccaa gtcctgccag aaacgcctgc
     6481 tcgagcagta atcctgcaaa ctgacggtta cgaaagcgcg gcgacaatac ccgttctttc
     6541 accacctccg gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga
     6601 tgcgtggtca gttgttccgg ggtcaacatc tcaatatccg aggcgctata aagcagaatg
     6661 gttttgtcag ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg
     6721 gtatcaacca cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taattcgttg
     6781 gaaaaatgag ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata
     6841 agttgtgatt gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg
     6901 cctttgatcg ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg
     6961 gtgatatcct tttttcattc tttattaagc ggggttatgc tgcgacaaca atcccctttt
     7021 tggcaaatgt agctcgtaat cggcgggcga acgccagagc gtgctcgccg tcgccatgca
     7081 ggcagaccgt ttgcgccgcg accgttgccc attcgccggt gatacttttg actctgccgt
     7141 gttgcaccat ctccagcgtt tgcgccagcg cctgttgttc gttttcaatc aacgcgcctg
     7201 gctgacttcg cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct
     7261 cgcgcgttgt caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa
     7321 cgagaatcaa tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg
     7381 cttctttcgc cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc
     7441 cttgcgcacg ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg
     7501 tttctggcgg caactgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt
     7561 gagcgccaat tgcaacacca ttttttattg cttcacgcac gcaagcctgc atggtttgcg
     7621 catcgcctgc atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg
     7681 cgtcgctggc gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc
     7741 cacgctaatt gttcaaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt
     7801 gaacactgga caaaatgaat cggctgaccg agcggaattt gcgcaagatg gtacatatca
     7861 gcctcaatga tgcaggcaat acgcgggtaa ccaccggtgg tctgtgcgtc gttcatcaac
     7921 acaatcggct gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac
     7981 agttcgcgat cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta
     8041 ctttgcaagc taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac
     8101 tcatgatatt ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg
     8161 ccctgcgctt ccataaaatc acgcttcgct ttgccaatcg ggagtcggtc accatccttc
     8221 agtaaacggc cttccagccc gccactcccc actttgagat cggtgctgca tgaccccatt
     8281 accggcggaa catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg
     8341 cgtttaagcg ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca
     8401 ttatcatcca gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg
     8461 aactcaacag ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc
     8521 accagtaggt tagcaatgcg taacgcgggc atatccagtg cgccgcagtg gctgataccc
     8581 gactggcgaa aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata
     8641 atcttcagca tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata
     8701 agatgggttc gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc
     8761 agccacccgg cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac
     8821 ctgccggaac gagtaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac
     8881 tcccgagata cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg
     8941 agtgcaattc aacaacctgt ttttcgctca acccgcaatg cgccgcgacc accgccaaat
     9001 ccggtccgcc tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca
     9061 gggcctcgct ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact
     9121 caggattacg caaaatcacc gtaatattgt tcatgccggg aatggcttca accacattcg
     9181 gcatatccac cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg
     9241 gcggttccag ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca
     9301 ctttcgatcc ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca
     9361 agatcggtat tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca
     9421 tggtgacctg cagcataaaa atgcaatccc tgctcgcggg ctgaatgaat ggtctgttca
     9481 gaaacttcgc cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt
     9541 tgcccgccgc ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac
     9601 cataacggct tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca
     9661 ggcatggtca gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc
     9721 agtaacgccg ccagctgtgc gttattgccc agctcaggat gtgcgtcaag cggcaaatgc
     9781 cagccataca ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg
     9841 cgaatgaccg gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg
     9901 cccagacgca ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt
     9961 tgcaccgtct ctttgccttc cacctgcaaa ccattcggcg cgtaatcact aatcgccgcg
    10021 ctgttcagtt tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc
    10081 gtattctgat agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc
    10141 ttttgacata ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg
    10201 cttcagctaa tgtatattta tgcaatcaga cagcatattg cataaataat cagttctgta
    10261 tcaaagtttt aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgccg
    10321 tcaattgtgg aattattatg aataaacacg catcacaacc gcgcgctatt tactatgtcg
    10381 ttgcgctgca aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct
    10441 atctcaccaa tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact
    10501 gttcgctggt gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca
    10561 atcgcatggc ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg
    10621 ccagtgagat ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg
    10681 gtctgtttaa atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc
    10741 gtcgtgatgg cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct attatcgcac
    10801 ctatcgcctg tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg
    10861 cggtgggtat gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcacacata
    10921 cccgcggcgt taataaaaaa gtactgcgtg caacaaactt tctcctgcct aactggggat
    10981 ggctgctggt tctgctggtg gcaacgcctg cgctgattac cgtactgttc tggaaagagt
    11041 ggtcggtata cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt
    11101 atcgcaaagc agaaaaccag aaacagcgta aggagctggg gctgattgtg acgctcacct
    11161 tcttcagtat gttgttctgg gccttcgcac aacagggcgg cagctcgatt agcctttata
    11221 tcgaccgctt cgttaaccgc gatatgtttg gttataccgt tccgactgct atgttccagt
    11281 cgattaatgc cttcgcagtt atgctgtgcg gtgtgttcct ggcgtgggtg gttaaagaaa
    11341 gcgtcgcagg taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga
    11401 tgagcgccgg attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact
    11461 cttctctgcc actgatggta ttaggcctgg cggtgatggg ctttgcggaa ctgtttatcg
    11521 acccggttgc catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa
    11581 ccggcatcta catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg
    11641 atcagacatc gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat
    11701 atattgaagt atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga
    11761 tgatttggct gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat
    11821 aggccagcac catacctgat aaaacgctcg ctgcgtttta tcaggctcct ttcctcgctt
    11881 cttcgtacgc tgccagcgtt cgcagccttg cctctttgtg atcaactatc ggttgcggat
    11941 aatccagcat cacacctgct ttctgcgccc acttccacgg ctcatgcacc gctttccctg
    12001 gtacatcgcg cagctccggt aaccatcggc ggataaactc accttcgcga tcaaatttct
    12061 cgccctgggt tgtcgggttg aaaatacgaa aatacggcgc tgcatcagta cccgtagacg
    12121 ccgcccactg ccagccaccg ttattggctg ccaaatcacc atcaatcagc tgcgacatga
    12181 aatatcgctc gccttcgcgc cagtcgatca acaaatcttt caccagaaaa ctggctgaaa
    12241 tcatccgtag ccggttatgc atccagccag tgctgttaag ctgacgcatg gcggcatcga
    12301 caatcgggta tcccgttttg ccttcctgcc aggcctgtaa atgtgcggga ttgctctgcc
    12361 actgcacacg atccgtccag gcaataaacg gacaatgttt acacaacgag gggtaatacg
    12421 tcatcagatg acggtagaac tcgcgccaga tcagctcact aagccagaca ctaccggccc
    12481 caccgtccag cgcctgcggc tgttccgcca acaagcgatg caaacactgg cgaggcgata
    12541 acccgcccgt tgccaggctg gcggacaaac gactggtgcc ttccactgcc ggaaaatctc
    12601 gttgttgctc atattctccg gcaccgttct ggcaaaactg gcgtaattgc gcaatcgccg
    12661 ctttttcttc caccgggaaa tgcgcagtat cgaaagactg acgaggataa ttcagcgtaa
    12721 tggatggcga gggctctatc gatccgctac tacgaacttt tggcgcagcg acgcactccg
    12781 gcatcccttc ccgcagccgt ttcagccagg cattcttaaa aggcgtaaag actttgtaca
    12841 tctcgtgatt accggtcttc accgcgccag gcggcaggat cacgctgtca tcaaatcctt
    12901 cacacaccac gttacgcagc gctctttcaa cttccacatc ccgcgcccgc tcattcactt
    12961 catactgata gttataaaac aggtgggtaa cgctgttttc cgcgcacacc tgtttaacta
    13021 tttcgacact ggcgacaaag tcatccactt cacggaacaa taaaggaata cctttttccg
    13081 caagcgctat ttgtagccca ttcagttgag cattgataag ttcagcctga cgcggcgaca
    13141 tgttatgcgt cgcccactgg cgtggtgtag cgatatacaa cgccagcacg cgtgcagacg
    13201 aattgcggca ggcggcagcc agtgcgagat tatcgtgcag acgtaaatcc tggcgaaacc
    13261 agaccagatg ggtagtcata aaactcctga atggcgcaag ttaatcacac gcggccaaag
    13321 ttcgaaataa cgctgccctg aaagccaggc gtcaggataa agcgccatat agtgtttgat
    13381 acgcatcagc ggcgcaagaa gtggttgctc gccacggcga taactgtcga taagcgccgc
    13441 caactgctgg cgttctgtgg aatcaatatg cgggcgaaaa taaccctgaa cgtgcatcaa
    13501 aacgttggtg tgatcgcgcg ggttggcggg atgagaaagc aaaacaatta cgcgttggta
    13561 gtactggttg taaaaatcat ccagattttg ccactggtga atatcagcca cgaacgggcc
    13621 aagtttgcgg tactccggct gagaatgcgc cagaaagacg agtttatagc ggctgtgata
    13681 atcgagaagc gccccgcgcg ttaagccatg ttctttcagt cgatgtaact catgaagggc
    13741 gaagatacgg attagggtgc tgtcatcaaa tcgttgctga ttcattactg ctcctgaacg
    13801 gtttacagga gtaagtgtag acgttgagaa atgatatttg tgaagttaat cctgttgtag
    13861 caggacaaat ttacgaaagc ctgctccccg caaggactga cgtcaggtag tttctaacca
    13921 tagctgccct ttctattcaa acgtgaaatc aataagctca tcagggacat cgcaaacaat
    13981 atgaagacgg gtttcttctc tgccgtgact cttttcacta attgcgtaac tgtcatgctt
    14041 gggattattt aattctttca gcggaaattt ttcctcgaag gctttattta gccgcccctg
    14101 gtttcctttt acagcgaata aataatcacc tccctgtttt tgtatcttct ctgcaatatc
    14161 tttctggcaa cccatcgcat cggttgtgat gatttttcct ttaatatcca gcatgttaag
    14221 aagttctggg atagctgtga tctcattgga tttcttatcc gtcttgatct gcccgatgac
    14281 cagactgtgc attgttgaga acgcactaat gacatgaatc gctcccctgc ggcgactctt
    14341 gtcataagag tgccggagcg tttttccatc aattgcaatg acgtctttat catttgatga
    14401 atggcaatca cgcatccagt taataaagca ctcgtgaaat tttgcaggac tgatacagga
    14461 tacaactctg gcaatagtat cgtg
//
LOCUS       DAZKGS010000115        14218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKGS010000115 DAZKGS010000000
VERSION     DAZKGS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHOQY_003425"
     CDS             complement(<1..209)
                     /locus_tag="ACHOQY_003425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679468.1"
                     /translation="MTKTKRVRLLALTEEGKAGNCFTVGKVYTGLLDTYTRLMHVTDD
                     NGNDQTIVLDGNCVFGEWELTTEKS"
     gene            complement(209..517)
                     /locus_tag="ACHOQY_003426"
     CDS             complement(209..517)
                     /locus_tag="ACHOQY_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679469.1"
                     /translation="MSEVHNVTIQMMDLVIDLQKAVNAALTFPSELTITQAVINLEST
                     REALQLLIAACEESEGAQIEADDLSNLIIVIHDTTEIMKEAVADLQPLINELIDKANN
                     "
     gene            complement(517..738)
                     /locus_tag="ACHOQY_003427"
     CDS             complement(517..738)
                     /locus_tag="ACHOQY_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679470.1"
                     /translation="MINKAILETTTFHDLKLLSECFISLIERAQEENDLGLLDDIIDN
                     IATYKIPLTIAMKKAREAQQQLEQSEEVA"
     gene            complement(780..1160)
                     /locus_tag="ACHOQY_003428"
     CDS             complement(780..1160)
                     /locus_tag="ACHOQY_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679471.1"
                     /translation="MSKKKGKKNVITTIGEACDQYALNNPRKYNYEHDAQPGEELIFI
                     LEEPSPELTDEELSLIQSMDGVYFNPRPRKTTVIDSSLLKRWQCLGFQDMHHFRGYMA
                     LNSLQYAGVRETELLLLDKIKIDD"
     gene            complement(1282..1488)
                     /locus_tag="ACHOQY_003429"
     CDS             complement(1282..1488)
                     /locus_tag="ACHOQY_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679472.1"
                     /translation="MTIFDSEFSQETFLQYLRERTGMILFDSEFSGVVGEQFLYALLD
                     SLSKQGLLQYTKTHMKAFRIDSVA"
     gene            complement(1485..1742)
                     /locus_tag="ACHOQY_003430"
     CDS             complement(1485..1742)
                     /locus_tag="ACHOQY_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679473.1"
                     /translation="MKYNLTLNKRTFEITESGYTLAKQLADSNYELPAIISFQELPHS
                     KEHTQHWLNHLSHFGVIKYEAVNSQTFRLLEISPFELWVAL"
     gene            complement(1755..2420)
                     /locus_tag="ACHOQY_003431"
     CDS             complement(1755..2420)
                     /locus_tag="ACHOQY_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9679474.1"
                     /translation="MNQNTKTIAEAKAQCLNKKDSKQTYVVVEQWLKMFKKWFSPSGK
                     EYTATLPAVEIYSYVAGFNEQGQECYANQKTLANIGMVSDRQVRNIINMFIDMGVLSV
                     EARDGQTSLYKALPFTDAHITPPEDVKTSVNVEQPAPETQEPLEATHSIEQETQAEIT
                     EQSDLFTWLISIDSQKPMQEETENFIKRALTSKRIILEDDAFNALMIALRKESEIYDS
                     IPF"
     gene            complement(2568..2750)
                     /locus_tag="ACHOQY_003432"
     CDS             complement(2568..2750)
                     /locus_tag="ACHOQY_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679475.1"
                     /translation="MKWYTPEHVVEAFKKGELTRHQVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
     gene            5067..5618
                     /locus_tag="ACHOQY_003433"
     CDS             5067..5618
                     /locus_tag="ACHOQY_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9679476.1"
                     /translation="MANIGYIRVSTVQQNTDRQLAGVALDKVFEDKASGKNTQRPQFE
                     AMMNYVREGDVLHVHSIDRLCRNTADLLATVEQLTERGVSVHFHKEDFKTGKNSPAGN
                     MMLTVLAAVAQMEREMMLERQREGIAAAKAAGRIAKRGNGKAIDRAGIAAALANGGSI
                     RSVAKDFSVSTQTVQRIKKEQEA"
     gene            6271..6591
                     /gene="yecR"
                     /locus_tag="ACHOQY_003434"
     CDS             6271..6591
                     /gene="yecR"
                     /locus_tag="ACHOQY_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HFP9679477.1"
                     /translation="MRKSLMLLLASTLIISGCATKKQLVPTGGSKSDGTVRMSYSYGM
                     FESPVVDPQQGMNAAKARCAAWGYSGAEPFGGFTSQCSQPSSSGCMQTLVTIEYQCTG
                     DIKK"
     gene            complement(6625..6882)
                     /locus_tag="ACHOQY_003435"
     CDS             complement(6625..6882)
                     /locus_tag="ACHOQY_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679478.1"
                     /translation="MVSIVYNNLTIPLHEVDMTVSYKITYEHRDGDQIRMEEVLFHSE
                     HEPTKDEIHDAVRKDTVRFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(7329..7402)
                     /locus_tag="ACHOQY_003436"
     tRNA            complement(7329..7402)
                     /locus_tag="ACHOQY_003436"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7368..7370),aa:Gly,seq:ccc)
     gene            complement(7481..8236)
                     /gene="actS"
                     /locus_tag="ACHOQY_003437"
     CDS             complement(7481..8236)
                     /gene="actS"
                     /locus_tag="ACHOQY_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9679479.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            8651..10948
                     /gene="xdhA"
                     /locus_tag="ACHOQY_003438"
     CDS             8651..10948
                     /gene="xdhA"
                     /locus_tag="ACHOQY_003438"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9679480.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            10959..11837
                     /gene="xdhB"
                     /locus_tag="ACHOQY_003439"
     CDS             10959..11837
                     /gene="xdhB"
                     /locus_tag="ACHOQY_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9679481.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            11834..12313
                     /gene="xdhC"
                     /locus_tag="ACHOQY_003440"
     CDS             11834..12313
                     /gene="xdhC"
                     /locus_tag="ACHOQY_003440"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9679482.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12353..14131)
                     /locus_tag="ACHOQY_003441"
     CDS             complement(12353..14131)
                     /locus_tag="ACHOQY_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9679483.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
BASE COUNT         3873 a         3264 c         3031 g         4050 t
ORIGIN      
        1 caactttttt cggttgtcag ttcccactca ccaaatacgc aattaccatc taaaacgatt
       61 gtttgatcgt taccgttatc atcggttacg tgcatcaggc gggtgtaagt gtcgagtaag
      121 ccggtataca ctttacctac tgtgaaacaa ttacccgctt tcccttcttc tgtgagcgcc
      181 agaaggcgca cacgtttagt ttttgtcatt agttgttagc cttatcgatt aattcattaa
      241 tgaggggttg cagatctgct accgcttctt tcatgatttc agtggtgtca tgaatgacga
      301 tgatcagatt agaaaggtca tcagcttcaa tctgtgcacc ttcgctctct tcgcacgctg
      361 caataagtag ttgcagtgct tcccgcgtcg attccagatt aataaccgcc tgtgtgatgg
      421 ttagctcgct tggaaaagtc agagccgcgt ttactgcttt ctgtaaatca ataaccagat
      481 ccatcatttg aatagttacg ttgtgtactt cgctcattat gccacttcct ctgattgttc
      541 taattgttgt tgtgcttcac gagctttctt catagcaata gtgagcggga ttttataagt
      601 tgcaatgttg tctataatat catcgagtaa accgagatcg ttttcttcct gtgcacgttc
      661 aatcaggctt atgaagcatt cagaaagaag tttaagatcg tgaaaagtgg ttgtctctaa
      721 aattgctttg ttaatcattt tattttcctt ttgttaaggg ttatattcag caaacgctgt
      781 taatcgtcga tttttatttt atcaagcaga agtaattcgg tttctctcac ccctgcatat
      841 tgcagactgt taagagccat atacccccta aagtggtgca tatcctgaaa acccaaacac
      901 tgccatcttt tcagaagact ggaatctata accgttgttt tgcgtgggcg cggattaaag
      961 taaacaccgt ccatactttg aatgagtgat aattcttcgt ctgttaattc tgggcttggt
     1021 tcttcaagta taaagatcaa ttcctcgcct ggttgtgcat catgctcata attatatttg
     1081 cgaggattgt ttaacgcata ctgatcacat gcttcgccaa tagttgttat tacgtttttc
     1141 ttgccttttt tcttgctcat tgcttcccct tcgttttgtt tttcttgttg cagtgttaaa
     1201 aaaggaagcg atcataaata ctaagaaaaa tattaagctt tccattgttt cacccttcaa
     1261 gatgtttgat agttgtttgt tttaagctac agagtcgatt ctgaatgctt tcatatgagt
     1321 tttggtatac tgcaataatc cttgtttact taggctatcg agaagagcat ataaaaattg
     1381 ctcacctacc acaccggaaa actcagaatc aaacagaatc attcccgttc tttcccgtaa
     1441 atattgcaga aaggtttctt gtgaaaattc gctatcgaat atggtcacag cgccacccac
     1501 aattcgaaag ggcttatctc aagcaaacgg aacgtctgac tattcacagc ctcatactta
     1561 attaccccaa aatggctcag gtgatttaac cagtgctgtg tgtgttcttt actatgaggt
     1621 aactcctgaa agctgataat cgcgggtagc tcataattgc tatcagcaag ctgttttgcc
     1681 agtgtgtagc cgctttctgt aatttcgaat gttcgtttgt ttaatgtcag gttgtatttc
     1741 attttttatt ctccttaaaa tggtattgag tcatagattt cactttcttt tctcagggct
     1801 atcatcagtg cattaaaagc atcatcttca agaattatcc tcttcgaggt cagtgcacgt
     1861 tttataaaat tttctgtttc ttcttgcata ggcttttggc tgtctatgga aataagccat
     1921 gtaaaaagat cagattgttc cgtgatttct gcctgtgttt cctgctcaat agagtgtgtc
     1981 gcttctaacg gttcctgtgt ttcaggtgca ggctgttcta cgtttacaga ggtttttacg
     2041 tcttccggtg gtgtgatgtg tgcatctgta aagggtaatg ctttatagag tgatgtttgc
     2101 ccgtcccgtg cctctacaga aagaacaccc atatcaataa acatatttat aatattacgc
     2161 acctgacgat cactaaccat tccaatatta gctaatgttt tttgattggc gtaacattct
     2221 tgcccttgtt cattgaaccc agctacgtaa ctgtagattt caacggctgg aagtgtcgcg
     2281 gtgtattcct tacctgacgg tgagaaccac tttttaaaca tcttcagcca ttgttcaact
     2341 actacatatg tttgtttact atctttctta ttgaggcatt gcgcttttgc ttcggcaatt
     2401 gttttggtgt tttggttcat ttagttcctc ctttgtaagg actgtaatta gtaattcatt
     2461 aacaagtttt ggaattgatt tcccttctgc ctttgcacga tttaacaacg cgttataagt
     2521 tgcagaatca aaattaatat tcatttggtt ttctccttct cctgttatta ttctttctct
     2581 gcttctgcct tacgcagttc atcaataatt tttagtgcat cgtcaaagca tttttcacgc
     2641 tctggataac cacagcgccg cgctgtgttc ctgttcatcc ttacttgatg tcgtgttagc
     2701 tcaccttttt taaatgcctc tacgacgtgt tccggtgtat accatttcat aaaaaactcc
     2761 ttatttagat aaaagaaaaa cacaccttgc agcctgacgg ctcaaagtgc agtgttctgt
     2821 gatttgatta tttagatgga gagagatgtt tctcccctcc ctacttacaa tactacataa
     2881 cggcggcttt gtcaaccctc tcttataaaa aacttgatca ttttcaattt cttataattt
     2941 ttcttagctt tttgtgctgc ctactctggt acaattcttc cctgttgcct aaaacgctct
     3001 caatcgcact ctgacggggg ttttgataaa aacaatacat tcccttatgt agaggttgtt
     3061 tatctcgtca gaggcttatt taaggggctt atttttgatt tatcagatga tggaaagatg
     3121 gtggatagat aaactctgat ttcagacatc ttataaaata cctgtaaagc tcctcagaag
     3181 ctctataacg agctaatcta ttaccgctaa gtgtttccct accttatata cgtttagctc
     3241 gcctacggct tgatttaagc ggtagaaact tatttaacct tcaactacta aaaccattct
     3301 ttcttttttg tttgttgttg gttgttgtct gtgtctatgt gctaacatcg ctaacgcgta
     3361 gcacacaagg ttattgcact cgtcagagta ttgcacaata gattttctct taaatagatt
     3421 gatgatcgtt agattggcgt aagcctcatc aatggaacta ttgaagcgca cagcgcatag
     3481 acactaacaa ccattcgctt acgctcaggt tattgcacaa gtaaatttca atcgttagat
     3541 ggtagttagt taattatcgt tctgcgcaca gcgcaagact attagccata cgcaagccgt
     3601 aggctatttg cacatagtag attacatatt cgttgtgagc ggaaagcgaa catagatcag
     3661 ctaactacta acaaaaaaca aaaaacaaaa aacaaaaact atctgttatg acaagcatca
     3721 gtctaacttg tgcaaggttc tggcgaatgc caatagatta agggatagtc gaagactccc
     3781 taacgggtat ccctataatt attctttaaa agataaaaga atttataaaa gatagtttaa
     3841 ttgtagttta attatagtat tgtaggaact catttcctgg gtaggtagga actcatttcc
     3901 tgggtaggta ggaaacccgc actatacggt aaaaagtgct tgcatgtcaa cttatattat
     3961 gttaattgcg gctttagatc gtctaagtga ttgttaaaag taacttttaa agttaacaat
     4021 tgatcaatca aacttaaaaa ttcccggctt tagaaaagca taagtattgt gcacactggt
     4081 aagtgtcttg cacagaaaaa ggtgtaaaaa atgtctaaag ccctttagat aaaaatgata
     4141 cctttataca tgcgctaata gcgcgcataa caataaccta ctaacaacta actaaaaagt
     4201 aagaagatgt tgttatgatt agtgtatcgc tcaacgctca catctatctc tgatctataa
     4261 attggtaact gtctaacaac aacaaacaac tattacatgc aaaacagcct tacggctgca
     4321 tagagatgat tggctaacct tcggttgcta cgcatagcca tatctatgag aagataaaag
     4381 agatttaaag gttagtttgt tgttgatcta actaacattc gctatacgct catacaatct
     4441 acattacaca ttaactatca aactacaatc taacgatata tagaatctat atccactcat
     4501 tatcattcgt gcaatgatca cattgatagt tcgctatacg ctcacctcta ttatgtgcaa
     4561 tggcttttca ggcaacgcgt tagcgtattc ttgcgaatgc ctgatctcca aagccggaat
     4621 taaacagcaa gaatatctcg tgtaaatcat agaatattca tagtttatcc ctatgtaatt
     4681 ccatactaaa aattcaatga aaagtagctt aaaagagcaa tgttagcttt cttcactttt
     4741 acattatcat cagcaaagcc ttatgtaatg cggcatatag cgcatcactg caaaattgtt
     4801 gctcaataaa cgttctatca tttattgaac aagtttcctt taatgcaaca aaaaattatt
     4861 ttttcaaaat aaacacacta tttaatagct tgctaatttt accaaaatgt aactacatga
     4921 ttctcatgca agacgtgtag tgataaagca ggctaaatca ccacactaaa aaccttcgaa
     4981 tttcgtagtg aaaaaagtcc tttacacacc taatcaacac gatcaataat cactacacac
     5041 aaatcaacac agaaggaata aagaaaatgg ctaatattgg atacattagg gtatcgactg
     5101 tgcaacaaaa cacagaccgt caattagctg gagtagctct tgataaagtc tttgaagata
     5161 aagcgtcagg caagaatacc cagcgcccac agtttgaggc aatgatgaac tatgtgcggg
     5221 aaggtgatgt tttgcatgtt cacagtattg atcgactttg ccgtaataca gccgacctac
     5281 tggcaacggt tgagcaatta acagagcgcg gcgtgtctgt gcatttccat aaagaagact
     5341 ttaagacagg gaagaactct cccgcaggga atatgatgct cacagtgctt gcagcagtgg
     5401 cacaaatgga acgtgaaatg atgcttgagc gccagcgtga ggggatcgcc gcagctaagg
     5461 ccgctggacg tatagcaaaa cgtggtaatg gaaaagcaat tgatcgagcg ggaattgctg
     5521 ccgctctcgc gaatggtgga tcaattcgga gcgtagcaaa agatttcagt gtcagcacac
     5581 aaacggtgca acgtatcaag aaagagcagg aagcgtaatg cctcctgata tttcagaatt
     5641 gtcgctgcat ggacctgacg gcgctgatct tctcgttaag cgatgatcac gtatgcgccg
     5701 atttcagaga aaaagacagt aactgaactc actatgaagg ctaaacgtat ctttacactt
     5761 gatggaaacg tccaaaaaac aacctaaccc caaaacccaa caaattgcaa aatattgttt
     5821 ttaaaagaaa atcaaatcta catcaaatat tggttgacgt agattgaggt gaatagtaga
     5881 ctattaggat aaattaccag tattgcacac aacaaatttg catgatttaa tccccctcac
     5941 attcatttag ggggcgttat gcatttatct tcaaaacatc ttagcactat ccgctttctc
     6001 gttattgctt cattcgttgt agctatcagt taccgacttt ttgttgcctc caatgacgaa
     6061 atgaatcctc ttgctattat tgcccattca atagcgacaa tagttagacg gtttgaaaaa
     6121 ccgtaataca ataaacatcc gaaataattt cgaaagcaac tcatttttac tctgcaaaca
     6181 atcagaaaag acttaatttt gctaagttaa tgcattaatc taaaaacgct ttaaatcaat
     6241 acataaccat tatctcttgg gattatttac atgcgcaaaa gtttaatgct gttacttgct
     6301 tcaacattga ttatttctgg atgtgcaaca aaaaaacaac tcgttccaac tggtggaagc
     6361 aaatcagacg gcacagttag gatgagttat tcttacggta tgtttgaaag tcctgttgta
     6421 gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg ccgcttgggg atattcagga
     6481 gccgagcctt ttggtggatt cacttctcaa tgttcacaac cttcttcatc tggttgtatg
     6541 caaacacttg tgactatcga atatcaatgc actggcgata ttaagaagta ataattaggg
     6601 gcttccagcc ccttctataa ccattcatgg aacagggaca accgaaatca ctttaaaccc
     6661 tgaaacagcc gttactccat tagatggaga attaaatctc acagtatctt ttcttactgc
     6721 atcatgaatt tcatcttttg ttggttcatg ctctgaatgg aataaaacct cttccattct
     6781 aatttgatca ccgtctctat gctcataagt aatcttgtag ctaacagtca tatcaacctc
     6841 atgcagggga attgtgagat tattatagac aatagaaacc ataaagcccc gttggtgata
     6901 cagcaacaag cgggagcgta gcgacttcat cagattgtca gaaagatcac accttatcag
     6961 gataaccgtg tacccgttgg gagcgtggct gaaacgctga tagagcttgc atatggtttg
     7021 caaggaccaa tatcaccata aacctgttta cgcagccaca aggccgcaca tcgggcttaa
     7081 atgcgtgtgt tcagtctgtc gatgacatta caatgccagg atgggcttta tcttcacgtt
     7141 gtgctatcgc ggtgtgagag gtgcaactgc cgccaacgtt aggttatact gtcatctcac
     7201 cgtgtaactt aacgtgacag ttactaaggg cggatcacaa tggtagaact cctaagcaaa
     7261 ttaaataggt aactgtttaa gttgctgaca gagaagactt ttttagtctt ctccacctgg
     7321 ggagattctg gagcgggcga agggaatcga accctcgtat agagcttggg aagctcttgt
     7381 tctaccattg aactacgccc gcttcgagat gcgtaaggca ttataaacct tacgctctcc
     7441 ttagcaagtg ccgcgctgct gactgctgat taattcgcca tcagcatttt ggcttgctgc
     7501 cctgcggcgg caagtaacgt agcggatcaa ttgccgttgc acggtaacga atctggaaat
     7561 gcaggcgaac agatgccgca tccgtgctcc ccatagtggc gattttttgc ccagccttca
     7621 cgctttgccc attatttacc agcatcgtgt cattatgggc gtaagccgta atgtaatctt
     7681 cactgtgttt aatcatgatg agattaccgt agccacgcag ctggttgccc acatacacca
     7741 cctttcctgc acccgcggcg taaataggtg taccccgtgg agctgagata tcaatccctt
     7801 tattgccgcc atctgctgtc gaatacggca tgataacttt ccctgtcgtt ggccataacc
     7861 aacaacgttg ccctactggc ggccaggaag atttcggtac cgctgatgac ggtgtaaccg
     7921 atgcggtttt ggtcgttgat ttggcggttg atttacgtgt actactgcta cttttcgccc
     7981 cacccagttt tagtttctga ccaacttcaa tggtgtaagg gggggaaatg ccgttcagtc
     8041 gcgccagttc ttttacgctg gttcccgtgg tgcgcgaaat acgatatagc gtatcccccc
     8101 gtttcacggt gtaaacggag ccggaatacg ttcctgtatc ggatgatttg ctacccgaac
     8161 agcccgccaa aagcagtcca accgataaca acatcacgat acccagagat tttttattca
     8221 ggcgtcccgc actcaaaatc tttcctcgct taaaaaacaa gacgccttat catagcagcc
     8281 agcctcttga tacccaatct cttgcttctg gaaaaacgtg gtaatagaga aagaggttaa
     8341 tttcatttgg caaagggaag tatcatcagc aaaatattcg ctgtagaggt atcgaacttc
     8401 ctgacttttt accctatttc ccatcctgat gcgtaataat cccaatttat cacattgatt
     8461 ttatcgccac tcatattgat acgtatcact gcccctattc agagtcctgt ttcgtatcat
     8521 atctgtgttt ttttaacgtc atcacacttc ctacctcccc taacgcttat cgtcgtttct
     8581 ggcgtaaatc ttgcctgctt agactaaatc tttgccataa gaaccaaatg ttcaaatcga
     8641 ggggatttct atggaagcgc gggaagcaac cgctacgggt gaatcatgca tgcgcgtcga
     8701 tgccattgct aaggtcaccg ggcgggcacg atatactgac gattatgtta tggcgggcat
     8761 gtgttatgcg aaatatgtac gtagccctat cgcacatggt tatgccgtaa gtattaatga
     8821 tgaacaagcc agaagtttgc cgggcgtact ggcgattttt acctgggaag atgtgcctga
     8881 tattccattt gctactgctg ggcatgcctg gacacttgac gaaaacaagc gcgataccgc
     8941 cgatcgcgca ctgctaactc gccatgttcg tcatcatggt gacgccgttg ccatcgtcgt
     9001 ggcccgcgat gaactcacgg cagaaaaagc ggcgcaattg gtcagcattg agtggcaaga
     9061 attacccgtt atcaccacgc cagaagcggc gctggcagaa gacgctgcac caatccataa
     9121 cggtggcaat ttactgaaac aaagcacgat gtcgacgggt aatgtccaac aaacaatcga
     9181 tgccgccgac taccaggtac aggggcacta tcagaccccc gttattcaac attgtcacat
     9241 ggaaagcgta acatcgctgg cgtggatgga ggatgactcg cgaattacca tcgtttccag
     9301 cacccagatc ccgcacattg ttcgccgcgt ggttggtcag gcgctggata ttccctggtc
     9361 atgcgtacga gtcatcaaac cgtttgtcgg tggcggtttt ggtaataaac aggatgtact
     9421 ggaagagcca atggcggcat tcctgaccag caagcttggc ggcattccgg tgaaagtttc
     9481 ccttagccgt gaagagtgtt tcctcgcaac ccgtacccgc cacgctttta ccattgacgg
     9541 gcaaatgggc gtgaaccgcg acggaacatt gaaaggttat agtctggatg ttctgtctaa
     9601 caccggcgct tatgcatctc acgggcactc catcgcttct gcggggggga ataaagtcgc
     9661 ttacctttat cctcgttgtg cctacgctta cagttcaaag acctgctata ccaacctccc
     9721 ctcggctggt gcgatgcgtg gttatggcgc gccacaagtc gtatttgccg ttgagtctat
     9781 gcttgatgac gccgcgacag cgttaggtat tgatcctgtt gaaattcgtt tacgcaacgc
     9841 cgcccgcgaa ggagatgcta atccgctcac gggcaaacgt atttacagcg cagggttgcc
     9901 ggagtgtctt gaaaaaggcc ggaaaatctt tgaatgggaa aaacgccgtg cagaatgcca
     9961 gaaccagcaa ggcaatttgc gccgcggcgt tggcgtcgcc tgttttagct acacctctaa
    10021 cacctggcct gtcggcgtag aaatagcagg cgcgcgcctt ctgatgaatc aggatggaac
    10081 catcaacgtg caaagcggcg cgacggaaat cggtcagggt gccgacaccg tcttctcgca
    10141 aatggtggca gaaaccgtgg gggttccggt cagcgacgtt cgcgttattt caactcaaga
    10201 taccgacgtt acgccgttcg atcccggcgc atttgcctca cgccagagct atgttgccgc
    10261 gcctgcgctg cgcagtgcgg cactattatt aaaagagaaa atcatcgctc acgccgcagt
    10321 catgctacat cagtcagcga tgaatctgac cctgataaaa ggccatatcg tgctggttga
    10381 acgaccggaa gagccgttaa tgtcgttaaa agatttggcg atggacgctt tctaccaccc
    10441 tgaacgcggc gggcagctct ctgctgaaag ctccatcaaa accaccacta acccaccggc
    10501 gtttggctgt acctttgttg atctgacggt cgatattgcg ctgtgcaaag tcaccatcaa
    10561 ccgcatcctc aacgttcatg attcagggca tattcttaat ccactgctgg cagaaggtca
    10621 ggtacacggc ggaatgggaa tgggcattgg ctgggcgcta tttgaagaga tgatcatcga
    10681 tgctaaaagc ggcgtggtcc gtaaccccaa tctgctggat tacaaaatgc cgaccatgcc
    10741 ggatctgcca caactggaaa gcgcgttcgt cgaaatcaat gagccgcaat ccgcatacgg
    10801 acataagtca ctgggtgagc caccaataat tcctgttgcc gctgctattc gtaacgcggt
    10861 gaagatggct accggtgttg caatcaatac actgccgctg acgccaaaac ggttatatga
    10921 agagttccat ctggcaggat tgatttgagg ataacatcat gtttgatttt gcttcttacc
    10981 atcgcgcagc aacccttgcc gatgccatca acctgctggc tgacaacccg caggccaaac
    11041 tgctcgccgg tggcactgac gtactgattc agctccacca tcacaatgac cgttatcgcc
    11101 atattgttga tattcataat ctggcggagc tgcggggaat tacgctggcg gaagatggct
    11161 cgctacgtat cggctctgca acgacattta cccagctaat agaagatcct ataactcaac
    11221 gtcatctccc ggcgttatgt gctgcggcca cgtccattgc tggaccgcag atccgtaacg
    11281 tcgctaccta cggtggaaat atttgcaacg gtgccaccag cgcagattct gccacgccaa
    11341 cgctaattta tgacgcgaaa ctggagatcc actccccgcg cggtgttcgt ttcgtcccga
    11401 ttaatggctt tcacaccggg ccgggcaaag tgtctcttga gcatgacgaa atcctcgtcg
    11461 cctttcattt tccgccacag ccgaaagaac acgcgggcag cgcgcatttt aaatatgcca
    11521 tgcgcgacgc aatggatatt tcaacgattg gctgcgccgc acattgccga ctggataacg
    11581 gcaatttcag cgaattacgc ctggcatttg gtgttgccgc gccaacgccg attcgctgcc
    11641 aacatgccga acagactgca caaaatgcgc cattaaacct gcaaacgctg gaagctatca
    11701 gcgaatctgt cctgcaagat gtcgccccgc gttcttcatg gcgggccagt aaagagtttc
    11761 gtctgcatct catccagacg atgaccaaaa aagtgattag cgaagccgtc gccgcggcgg
    11821 ggggaaaatt gcaatgaatc acagcgaaac aattaccatc gaatgcacca ttaacgggat
    11881 gccttttcag cttcacgccg caccaggcac gccgctctcg gaattactcc gcgaacaagg
    11941 actgctaagt gtcaaacaag ggtgctgcgt gggtgaatgt ggtgcctgta cggtgttggt
    12001 cgacggcaca gcaatagaca gttgcttata ccttgccgcc tgggctgaag gaaaagagat
    12061 ccgcacgctg gaaggtgaag cgaaaggcgg aaaactttct catgttcagc aggcttatgc
    12121 gaaatccggc gcagtgcagt gcgggttttg tacgcctggc ctgattatgg ctaccacggc
    12181 aatgctggcg aaaccacgcg agaagccatt aaccattacg gaaattcgtc gcggactggc
    12241 gggaaatctt tgtcgctgca cggggtatca gatgattgta aatacagttc tggattgcga
    12301 gaaaacgaag taaaaggata tccggcctga attcaggccg gattcactga ggttatgtgt
    12361 ttaacaactc atatttctta atcttgcgat agagcgtagc aatgccgatg cccagttcat
    12421 cagcaacttg cttcttgctg ttatgacgtg aaagcgcctc gcggatcatt tgcttttcca
    12481 tctcctccag cgccgtgccg cccgcatcat cgagtgacag gtgcgcctca ctgacctctg
    12541 ttacatcact ttgctccgtt gtgccattat tcagcagatt tggcggcaat agcgtgctgt
    12601 cgataacttc acctgaagga actacgttaa ccagatattc catcaaattg cttaactcgc
    12661 gcaggtttcc gggccaacga tgcttacgca atatttcgac gacatcggga gcaatgccag
    12721 gataaaccga tcccagacga cgggtatgca gatgtaaaaa gtaatgcacc aatagttcaa
    12781 tatcttcctg acgttcacgc agcggtggca gagttatcgg gataacatta agtcggtaga
    12841 agagatcttc gcggaattta ccttcggcaa tgaactgggc caaattctga ttagttgcag
    12901 aaatgatgcg aatgtcgact tgtattgggc tactggcacc aatcggtaga atttcacgcg
    12961 cctcaatagc gcgcagtaat ttagcctgca acattaatgg catatcacct atttcatcga
    13021 gaaacagcgt gcccgtattc gccgcctgaa tcaaccctgt tttaccgttg gcagaagcgc
    13081 cagtaaatgc gcctttaaca taaccgaaca gttcgctttc cagaagctgc tccggaatcg
    13141 cggcacagtt gatagcaata aagggtttat tccgtcttcc gctcaactta tggattgctc
    13201 gggcgacgac ttctttaccc gtgccgcttt caccaaccac cataacgctg gatgggctgg
    13261 gtgcaatacg gctaatgagt cgttttaatt gccgcataac acggcactcg ccaaccaatt
    13321 gttcaatatg tggttcatca ggtgcatttg ctacagaaaa actggtatgc gattggtgaa
    13381 atgccattaa aaataattgt cggccctgaa tgttatgcaa ttgaccaatg attaattcac
    13441 ttttatcgtc ccacgaaaca atatgctgca tatgtccatg agtaaaatta ctctcaaatg
    13501 ttaatggtct gaaacggata ggtttcccaa taatattatt ttgtacaaca ccaagtgttt
    13561 ttaaggcagt ctgattaaca aactgaaccc gactttcatc atcaacaact aatacgccct
    13621 gatccatatt atcgatcatg gtcgcgaata ttttactgat gttatctcct ggcccctgat
    13681 cctccagaag tttcgaaaca aaaatggtgg atatatggcg aacataatca gaaaattcgc
    13741 gtaaattatc actgatatgc tcttgttgct cgtgggtaac ggcaatcaaa cttatcaccc
    13801 caacacaacg atcctgtaaa atgacaggcg tacccagaaa tgctttttca cggcaatttt
    13861 ctttactatc gcaaccttcg caaaggggat cgaagcgaga ctgtgtcaca actttttcag
    13921 ttttcgtttc caggacgtgg cggagcaggc gtgagttgcc gctcaactgg cgaccaagaa
    13981 acttcccata cgcgcccgtt ccggcgacgc gacacaagtt ttcatcaacg atctcaacct
    14041 caagctgcaa aacgctggca agcattctgg caaaacgctg aattgtcggt tgaatttgca
    14101 tcaatactga ctgcgtagta gcaagctcca tagctttacc ttccagactt acttaaaagt
    14161 cgatcattga agacgttgat ggttcacaga tcatgatgat attaactcag gcgaaatt
//
LOCUS       DAZKGS010000116        14206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKGS010000116 DAZKGS010000000
VERSION     DAZKGS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..249
                     /locus_tag="ACHOQY_003442"
                     /pseudo
     CDS             154..249
                     /locus_tag="ACHOQY_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            273..4787
                     /locus_tag="ACHOQY_003443"
     CDS             273..4787
                     /locus_tag="ACHOQY_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9679484.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4788..6437
                     /gene="yfaQ"
                     /locus_tag="ACHOQY_003444"
     CDS             4788..6437
                     /gene="yfaQ"
                     /locus_tag="ACHOQY_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP9679485.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKQYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6442..7218
                     /gene="yfaP"
                     /locus_tag="ACHOQY_003445"
     CDS             6442..7218
                     /gene="yfaP"
                     /locus_tag="ACHOQY_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9679486.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7493..10342
                     /gene="rcsC"
                     /locus_tag="ACHOQY_003446"
     CDS             7493..10342
                     /gene="rcsC"
                     /locus_tag="ACHOQY_003446"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9679487.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10428..11078)
                     /gene="rcsB"
                     /locus_tag="ACHOQY_003447"
     CDS             complement(10428..11078)
                     /gene="rcsB"
                     /locus_tag="ACHOQY_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9679488.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11095..13767)
                     /gene="rcsD"
                     /locus_tag="ACHOQY_003448"
     CDS             complement(11095..13767)
                     /gene="rcsD"
                     /locus_tag="ACHOQY_003448"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9679489.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         3439 a         3464 c         3804 g         3499 t
ORIGIN      
        1 taccttgcgc taatgctctg tctcaggtca ctaatcacac ctaactcatt gattaattgt
       61 gactctaaat gtagtgtttt gtcgcgtttt gcagtatgtt atttaatcta ttatataaat
      121 aacataatga tttttaatgt atttttgtaa tttactctcc ttctgacatg gaaggacacc
      181 cgatggatac ccaacgattc caatcccaat ttcattggca tttatcgttt aaattttctg
      241 gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac agggcttgct aatgctgatg
      301 attcgcttcc ttccagtaac tatgcgccgc ccgccggggg aacattcttt ttgcttgctg
      361 acagcagttt tagcagcagt gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt
      421 atcggcgcta tcagatggaa gagtacggcg gcgtggacgt tcgcctgtat cgtattcctg
      481 acccgatggc atttttgcgc cagcagaaaa acctgcatcg cattgtggtg caaccgcaat
      541 atctgggcga cgggctgaac aatacgctga cctggctgtg ggataactgg tacggcaaat
      601 ctcgccgcgt aatgcagcgt actttctctt ctcagtcacg gcagaatgtg actcaggcat
      661 tacccgaatt acatctcggc aatgccatta ttaaaccttc ccgttatgta cagaacaacc
      721 agttttcccc gctgaaaaaa tatcccttgg tggaacagtt ccgttatcca ctatggcagg
      781 ctaaaccggt cgagccgcag caaggggtaa aactggaagg cgcatccagc aatttcatct
      841 cgccgcagcc gggtaacatt tatattcctc tcggccaaca agagccggga ctgtacctcg
      901 tcgaggcgat ggttggtggg tatcgggcga cgacggtggt gtttgtttcc gataccgtgg
      961 cgcttagcaa agtgtcaggc aacgagcttc tggtgtggac cgcgggtaaa aaacagggtg
     1021 aagcgaagcc cggctcagag atcttgtgga ctgacggtct tggcgtgatg acccgcggtg
     1081 tgaccgatga cagcggtacc ttgcagttac aacatatatc gccagaacgt tcatacattc
     1141 tgggtaagga tgctgaaggc ggtgtttttg tctccgagaa cttcttctac gaaagcgaaa
     1201 tctacaacac ccgcttgtat atttttaccg atcgcccgct atatcgcgca ggcgatcgtg
     1261 tcgatgttaa agtgatgggc cgcgagttcc acgatccgtt gcattcatcc cccatcgtca
     1321 gcgccccggc gaagctttcg gtgctggacg ctaacggcag tctgttgcaa accgtcaatg
     1381 tcacgctgga tgcgcgcaat ggcgggcagg gaagtttccg cctgccagaa aatgccgtag
     1441 ccggaggtta tgagttacgt cttgcttacc gcaatcaggt ctatagcagc agttttcgcg
     1501 tggcaaacta catcaagcca catttcgaga ttggtttagc tctcgacaaa aaagagttca
     1561 aaactggcga agcggtcagc ggcaaactgc aactgcttta cccggatggt gagccggtaa
     1621 aagatgcccg cgtgcagtta agtttgcgcg ctcagcaatt atcaatggtc ggtaacgatt
     1681 tgcgttatgc cggacgtttt cccgtgtcgc tggaaggcag cgaaacggtg tccgacgcca
     1741 gcggtcatgt ggcgttaaat ctccccgccg ccgataaacc gagccgctat ttgttaaccg
     1801 tctccgccag tgacggcgcg gcgtatcgcg tcaccaccac caaagagatc ctcattgaac
     1861 gcggcctggc gcattactct ttaagtaccg ccgcacaata cagtaatagc ggcgagtcgg
     1921 ttgtgttccg ttatgccgcg ctggaatctt caaaacaggt tcctgttacg tatgaatggt
     1981 tgcgtctcga agaccgcacg agccatagcg gagagctacc gtcaggcggc aaatccttta
     2041 ccgtcaattt cgctaaacct ggcaactaca atctgacatt acgcgataaa gacggcttaa
     2101 ttctcgctgg gttaagtcat gccgtcagcg gtaagggcag cacggcgcat actggtacgg
     2161 tagatatcgt ggcggataaa acgctgtacc agccaggcga aactgcgaag atgctgatta
     2221 cctttccgga gccaattgat gaagcattat tgacgctgga acgcgatcgc gtggaacagc
     2281 agtcgctgct ttcgcatccg gcaaactggc tgacgttaca acgtttaaac gatacccagt
     2341 atgaagcccg ggttccagtg agcaattcct ttgcgcctaa catcactttt tcggtgctgt
     2401 atacccgtaa tggtcagtac agttttcaga acgccgggat caaagttgcc gttcctcagc
     2461 ttgatatccg ggtgaaaacg gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg
     2521 aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg
     2581 atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc
     2641 cgctggggcg taacaatgtg cgtaccagct ccagtctgtc gtttatcagc tacgaccagg
     2701 cgctctccag cgagccggtt gcgcctggcg caactaaccg cagcgagcgg cgagtaaaaa
     2761 tgcttgaacg tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa
     2821 ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc
     2881 gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc
     2941 gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtgtatcgc gtgggcgaca
     3001 aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga
     3061 ctaaatttgc aggcgctgag atgcgccaga cgctgacgct gcacaaaggg gcgaattata
     3121 tttcgctgac gcagaacatt cagcaatctg gcttgttaag tgcagaactg caacaaaatg
     3181 ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt ggacaacagc tggcccgttg
     3241 aacagcagaa aaatgtcatg ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg
     3301 cgagtaatat ccggctacaa agtagtgaaa cgccgcagga gattttccgc aacaatcttg
     3361 atgcgttagt cgatggacca tggggtggcg tgatcaacac cggtagccgt ctgatcccgc
     3421 tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac gacattcgtc
     3481 agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga gcgcgcttta
     3541 cctggtgggg tgaagatggc aatggtgacg ccttccttac ggcatgggca tggtacgccg
     3601 actggcaggc cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc
     3661 tcgacagtta tgccgagcag gcggataaca tgccgttatt gcatcgggcg ctggtgctgg
     3721 cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta
     3781 tcgcccggcg cggaactaaa gatgaagatt tctctgagga agacatccgc gatatcaatg
     3841 acagcctgat cctcgataca ccggaatctc cactggcaga tgcggtggca aacgtcttaa
     3901 ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt gatgccacag gttcagcaat
     3961 atgcgtggga taaagcggca aacagcaatc agccgctggc gcacacggtt gtgctgctca
     4021 atagcggtgg tgacgctacc cagacggccg ctattttaag tggtttgacc gctgagcaat
     4081 ccactattga gcgcgcgctg gccatgaact ggctggcgaa atatatggcg acaatgcctc
     4141 cagttgtttt gcctgcgcct gcgggcgcat gggctaaaca taagttaact ggagggggcg
     4201 aagactggcg ttgggttggt cagggcgtgc cggacattct ctcttttggt gacgaattat
     4261 cgccgcaaaa tgtgcaggtc cgctggcgtg agccggcaaa aatggctcaa caaagtaaca
     4321 ttccggtgac cgttgaacgc cagttgtatc ggcttatccc tggtgaagaa gagatgagct
     4381 ttattctgca accggtgacc agcaatgaga ttgacagcga tgcgctgtat ctcgatgaaa
     4441 ttacgcttac cagcgagcag gatgcagttc tgcgctatgg tcaggtggaa gtaccgctcc
     4501 cgccgggagc cgacgttgag cgcacaacat ggggcatttc agtcaataaa cccaacgccg
     4561 cgaaacagca ggggcaattg ctggaaaaag cgcgtaatga aatgggcgaa ctggcctata
     4621 tggtgccggt gaaagaactg acgggaacgg tcactttccg ccatttgctg cgcttctcgc
     4681 aaaaagggca attcgttctg cctcctgctc gttatgtgcg ttcctatgca cctgcgcagc
     4741 aaagtgttgc ggcagggagt gaatggaccg ggatgcaggt gaaataagtg aactggcgca
     4801 gaatcgtctg gctattggcg ttggttactc tgccaacgct tgcggaggag acgccgttac
     4861 aactggtgct gcgcggtgcg cagcacgatc aactgtatca gctgtcgtca tcaggcgtga
     4921 ccaaagtctc agcattacct gactcgctca ccacgccgtt agggagtttg tggaaacagt
     4981 atgtttacgc ctggctggag gatacccatc aaccggagca gccctaccag tgtcgcggaa
     5041 actccccgga agaggtttat tgttgtcagg ctggggagag cataacgcgc gataccgcgc
     5101 tggtacgttc ctgtgggctc tattttgcgc cgcagcggtt gcatattggc gcagatgtgt
     5161 gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt agcgtctctt acaacgctga
     5221 agccagaaac ctcggtaacg gttaaatcgc tgctcgattc attagcgacg ttacctgcgc
     5281 aaaacaaagc tcaggaagtc ttactggatg tggtgctgga cgaggcgaaa atcggtgtcg
     5341 catccatgct cggtagtcgg gtacgggtga aaacctggag ctggtttgcc gatgataaac
     5401 aggaaatccg ccagggtggt tttgctggct ggttaaccga tggtacaccg ctgtgggtta
     5461 ctggcagtgg caccagcaaa acggtactaa cccgttatgc gacggtgctt aatcgggttt
     5521 tgccagtgcc gacgcaggtt gccagtgggc aatgtgtaga ggtggagctg tttgcccgct
     5581 atccgctgaa gaaaatcaca gcggaaaaga gtacgacggc agtaaaaccc ggcgtactta
     5641 atgggcgcta tcgtgtgacg tttactaatg gtaaccacat tacgtttgtc agccacggtg
     5701 agacgacctt actgagcgaa aagggcaaac tgaaattgca gtcgcatctc gatcgtgaag
     5761 agtacgttgc cagagttctg gatcgcgagg cgaaaagtac gccccctgag gcagcgaaag
     5821 ccatgaccgt ggcgattcgc acttttttac agcaaaacgc caatcgggaa ggcgattgcc
     5881 tgaccatccc cgacagcagc gccacgcagc gtgtttctgc ttcgccagcg accacggggg
     5941 cgcgaacgat gaccgcctgg acgcaggatc tcatctatgc aggcgatccg gttcattatc
     6001 atggcagtcg agctaccgaa gggacacttt cctggcggca agccacggcc caggcggggc
     6061 agggagagcg ttacgaccag atactcgcgt ttgcttatcc cgacaacagc cttagccgct
     6121 ggggagcgcc acgctcaacc tgccagttat tacccaaagc gaaagcctgg ctggcgaaaa
     6181 aaatgccgca gtggcggcgt atattacaag gcgagacggg gtacaacgaa ccagacgtgt
     6241 ttgcggtctg tcgtttagtc tcaggtttcc cctataccga tcggcagcag aaacggctgt
     6301 ttattcgtaa cttctttacg cttcaggatc ggctggattt aacccatgag tatctgcacc
     6361 tggcctttga tggttatccc accgggcttg atgagaacta tatcgaaacg ctgacccgac
     6421 aattattaat ggactaatgc tatgcgaaag atttttcttc cgttgttact ggtggcactt
     6481 tcgcctgtcg ctcacagtga aggtgtgcag gaagtcgaga ttgacgcacc gctctctggc
     6541 tggcatccgg cagagggcga agatgccagt ttttcacaat cgatcaatta cccggcgtcg
     6601 tccgtcaaca tggcagatga tcaaaatatc tcagcgcaga tccgcggcaa aataaagaat
     6661 tatgctgcgg cgggtaaagt tcagcagggt cggctggtgg tcaacggtgc cagtatgccg
     6721 cagagaattg aatccgatgg ttcatttgca cgcccttata ttttcactga aggcagcaac
     6781 agcgtgcagg tcatcagccc ggatgggcaa agccggcaaa aaatgcagtt ttactcaacg
     6841 ccgggtacag gaacgattcg tgcacgttta cggctggttc tctcgtggga tacggacaat
     6901 accgatctcg atctccatgt tgtcacgccc gatggcgaac acgcctggta cggtaacacc
     6961 gtgctgaaaa acagtggtgc actggatatg gatgtcacga cggggtacgg acccgagatt
     7021 ttcgccatgc cagcaccagt tcacgggcgc tatcaggtgt atatcaacta ctatggtggg
     7081 cgcagcgaaa cggaattgac gaccgcccaa ctgacgctga tcaccgatga aggatcggtc
     7141 aatgagaaac aggaaacatt tattgtaccg atgcgtaatg ctggcgagct gacgctggtg
     7201 aaaagttttg actggtgatc catcccttca cacaacctgg ctctctcttt tttcagataa
     7261 gactgctggg cgatctgccg acgccaggat ataaccatta acaagcgtcg gcagattggt
     7321 catttcttat ttttcgtact gagaactgtc catctactcc ggctttcctg gttaaggcaa
     7381 tggtttctaa gcgatacgca ttattgcaat ggtgacagaa cattgcggta acacgctttt
     7441 accgctacct taaccacact ccatcggtca cctgaggcgg agcttcgccc ctttgaaata
     7501 ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac atgttcagag cattggcgtt
     7561 agtgctctgg ctgttgattg ctttttcatc cgttttttac atcgttaatg cgttacatca
     7621 gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc gatcaggctc agcgctttat
     7681 tcaacgcacc tctgatgtga tgaaagagct gaagtacatc gccgaaaatc gcttatcggc
     7741 agaaaacggt gtgctttccc cgcgtggacg agaaacgcag gcggatgtgc ctgcgtttga
     7801 accgctgttt gccgactccg attgttccgc aatgagtaac acctggcgag gttctctgga
     7861 gtcattggcg tggtttatgc gctactggcg cgataatttt tctgcggctt acgatctcaa
     7921 ccgggtattt ttaatcggca gtgataacct ctgcatggcc aatttcggtc tgcgtgatat
     7981 gccagtggaa cgcgataccg cgttgaaagc gttgcatgaa cgcatcaata aatatcgaaa
     8041 tgcaccacaa gatgatagcg gcagtaacct ctactggatc agcgaaggtc cgcgccctgg
     8101 cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg aaccggttgc aggcgctttt
     8161 gggtgtcgag cagaccatcc ggatggaaaa ctttttctta ccgggtacgt tgccgatggg
     8221 ggttaccatt cttgatgaaa atggtcatac cctgatttcg cttaccggac cagaaagtaa
     8281 aattaagggc gatcctcgct ggatgcagga acgctcctgg tttggctata cggaagggtt
     8341 ccgggagctg gtgctgaaga aaaatctgcc accctcatcg ctaagcatcg tgtattcggt
     8401 gccggttgat aaggtgctgg aacgcattcg catgttgatc cttaacgcaa ttttgctgaa
     8461 tgtgcttgcc ggagctgcat tgtttactct cgcacggatg tacgagcgac gtattttcat
     8521 tccggcggaa agcgacgccc tgcgactgga agaacatgag cagttcaatc gcaagattgt
     8581 cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct gatggcgtca atattttaag
     8641 taacgaactg gcgcatacct atctcaatat gcttacgcat gaggaccgcc aacgactgac
     8701 gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc ctgaccagca acaataccaa
     8761 tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa aacgtggcca tttgtgtgct
     8821 ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg caggagatgg cacaagcagc
     8881 ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc gtcagtcatg agctgcgaac
     8941 gccgctgtat ggcattatcg gtaacctgga tctgttgcaa accaaagagt taccgaaagg
     9001 cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc ctgttgttga aaattatcag
     9061 cgatattctc gatttctcga agattgaatc ggaacagttg aagatcgaac cgcgtgagtt
     9121 ttcaccgcgt gaagtgatga accacatcac cgccaactat ttaccgctgg tggtacgcaa
     9181 gcagttaggc ttgtactgct ttattgaacc ggatgtgcca gtggccttaa atggcgaccc
     9241 gatgcgttta cagcaggtca tctccaacct gctgagtaac gccataaaat tcaccgatac
     9301 cggctgtata gttttgcatg ttcgcgctga tggcgattat ctctctatcc gtgttcgcga
     9361 taccggcgtg ggcattccgg cgaaagaagt ggtgcgcttg tttgatccct tcttccaggt
     9421 cggaacgggc gtacagcgta acttccaggg gaccggtctg ggtctggcga tttgtgaaaa
     9481 actgatcagc atgatggacg gcgatatctc ggtagattca gaaccgggaa tgggcagcca
     9541 gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg cagaaaaaag gcgtggaagg
     9601 gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg tcgctctgtc agttcctgga
     9661 aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac gaagggcagg aaccgactcc
     9721 cgaagatgtg ttaatcactg acgaggtagt gagtaaaaaa tggcagggta gagcggtagt
     9781 gaccttctgt cgtcgccata ttggtattcc gctggagaaa gcgccagggg agtgggtaca
     9841 cagtgtggct gctccgcatg agctaccggc attgttggcg cgtatttatt tgatcgagat
     9901 ggagagcgac gatcctgcta acgctctgcc gtcgacggac aaagcggtca gcgataacga
     9961 cgatatgatg attctggtcg tggatgatca tccgattaac cgacgtctgc tggcagatca
    10021 gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat ggcgtcgatg cgcttaatgt
    10081 acttagcaag aatcatattg atatcgtgct tagcgacgtc aacatgccaa atatggatgg
    10141 ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg ttgccggtaa tcggagtaac
    10201 tgctaatgcg ttggctgaag agaagcagcg gtgtctggag tccggtatgg acagctgcct
    10261 gtcgaagccg gtaacgctgg atgtgataaa acagacgctg acggtatatg ccgagagggt
    10321 caggaaatcg cgggaatcgt aggacggata aggcgtttac gccgcatccg gcattcagtg
    10381 caaatgccag ataagacact aacgcgtctt atctggccta caggtgatta gtctttatct
    10441 gccggactta acgtcacaga agagagataa ttcagcaggg cgatatcgtt ctcgacaccc
    10501 agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg gttcagcttt
    10561 ttagcgatct cggtcaccag gaagccttcc gcaaacaggc gcagaacttc actctctttt
    10621 ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag gcgagaaacg
    10681 ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg cagatcggtc
    10741 ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc actaagaatc
    10801 gccgggttgt tgttcatggt cagaacaata atcgacaggc ttgggaaatg gcgcttgatg
    10861 tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag atcggtaatc
    10921 aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg
    10981 ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa caagactatc
    11041 ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac cttgctacag
    11101 caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg gaacatcctt
    11161 ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca gatttagcat
    11221 ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt cactggttgc
    11281 tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga gcgcataata
    11341 gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg tcacctcttc
    11401 ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt
    11461 gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat catcgcttaa
    11521 aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat cataatcttg
    11581 actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta actggcgagt
    11641 gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat catccagtaa
    11701 acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat gcacagagta
    11761 gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt tacgcgccag
    11821 ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg
    11881 gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt ctccggtgtc
    11941 cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct caagggtgat
    12001 ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa
    12061 ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa tcagcagttg
    12121 cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa ttaaatcctg
    12181 cacggagaac agcaccgtct cacttttcca gctatcatcc gcaagcatgt tcgctaactg
    12241 tatttcatca accagccgca ccagcacatc tgcctgattc gccagttgtt tgctttccgg
    12301 ggcgttgagt ttagccgcgc tctccgccag ggactgtgca ggttctttca gcgcatcgcc
    12361 aatgtttttc ataaaggtca tccggccctg ctggtttttc tcatacagac gctgcgcctg
    12421 cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga aaatttgtgt
    12481 gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat tgatcgtcgc
    12541 ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat tcaaatgcgg
    12601 cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct gatcgtgaac
    12661 cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta aaatgcgcaa
    12721 ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga aatggcggaa
    12781 tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca gtggcagcag
    12841 aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga ccaggcgcat
    12901 atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt ggatactgac
    12961 gctatccgtc ccttctttct cattatcatt gtttcccgtt gccgtcgcgt ctggctcaag
    13021 gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggca gatcaaaagc
    13081 cacgaccgtt gccagatgtc ctggctggtt aaaggtagta cgcaaggtaa agtaatgacc
    13141 gttctgccag gccaggcggc gcatgttaga aaagctttcg cgttcatcga gggcgttggc
    13201 ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa
    13261 tccggaggtg agatctttaa ggggtagggt tgagatcagc accagactgt tatcctgacc
    13321 attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtat ccagataagt
    13381 ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga ttaacgcttc
    13441 cgttttgcgg cgtggtttct ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa
    13501 accttcgccg gaggaggggg aggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac
    13561 gtaacgccag gtatcgacgc gcttttggat agcgtgggta atgtcgacaa tctggtaact
    13621 tttatctttc agccatgcgt taacggcgct ttgtaccatt acacccatcg tcaccagtaa
    13681 cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt
    13741 cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt
    13801 gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat tgatgattct
    13861 ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca agcgccattt
    13921 tgctgtacaa ataacctaca aaaagcccaa cgaaaataat tgcaaataaa caaagattgc
    13981 tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat agtttttctg
    14041 tggtagcaaa gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccga atgcgaggca
    14101 tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata aataaagtta
    14161 atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttt
//
LOCUS       DAZKGS010000117        14197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKGS010000117 DAZKGS010000000
VERSION     DAZKGS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHOQY_003449"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHOQY_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9679490.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..2833
                     /gene="safA"
                     /locus_tag="ACHOQY_003450"
     CDS             2636..2833
                     /gene="safA"
                     /locus_tag="ACHOQY_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP9679491.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2908..3669
                     /gene="ydeO"
                     /locus_tag="ACHOQY_003451"
     CDS             2908..3669
                     /gene="ydeO"
                     /locus_tag="ACHOQY_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9679492.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4071..5753
                     /gene="ydeN"
                     /locus_tag="ACHOQY_003452"
     CDS             4071..5753
                     /gene="ydeN"
                     /locus_tag="ACHOQY_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9679493.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKQKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            5805..6962
                     /gene="ydeM"
                     /locus_tag="ACHOQY_003453"
     CDS             5805..6962
                     /gene="ydeM"
                     /locus_tag="ACHOQY_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9679494.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(6928..7050)
                     /locus_tag="ACHOQY_003454"
     CDS             complement(6928..7050)
                     /locus_tag="ACHOQY_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679495.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            7253..8938
                     /gene="yddA"
                     /locus_tag="ACHOQY_003455"
     CDS             7253..8938
                     /gene="yddA"
                     /locus_tag="ACHOQY_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9679496.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            8976..11348
                     /gene="yddB"
                     /locus_tag="ACHOQY_003456"
     CDS             8976..11348
                     /gene="yddB"
                     /locus_tag="ACHOQY_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9679497.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11405..14188
                     /gene="pqqL"
                     /locus_tag="ACHOQY_003457"
     CDS             11405..14188
                     /gene="pqqL"
                     /locus_tag="ACHOQY_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9679498.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         4114 a         3130 c         3144 g         3809 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     2821 tttgcaagac tgaaaataaa cagcaaatta taaatatgaa atgttaaaaa agtatcgata
     2881 aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat
     2941 catgccttca acgctaacat tttagataaa gattacgcct tctctgacgg cgagatcctg
     3001 atggtagata acgctgttcg tacgcatttt gaaccttatg agcggcattt taaagagatc
     3061 ggatttactg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
     3121 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
     3181 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
     3241 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
     3301 gtgctatccg tttccggaaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
     3361 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta
     3421 aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa
     3481 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
     3541 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
     3601 aaggagtacc gttgtcaaag tcacacgggt atgaatacgg gcaacacgat gagtgcttta
     3661 gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg
     3721 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
     3781 catccatcac atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt
     3841 cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg
     3901 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc
     3961 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     4021 ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg
     4081 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg
     4141 catttgctgc tcatgcggca gatgatgtaa agcagaaagc aaccaaaaca aacgttgctt
     4201 tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa
     4261 ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa
     4321 caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag
     4381 ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta cggtttacta
     4441 acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag
     4501 ctcccgcccg ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag
     4561 aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat
     4621 ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc
     4681 atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt
     4741 actttatggg attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa
     4801 atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg
     4861 gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg
     4921 ctccgcacct gccaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg
     4981 gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac
     5041 gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct
     5101 ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata
     5161 agagtcagac ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac
     5221 ttcaacccgg taattatgac aagctgattt ccgcaatgga tttctacccg acagctcttg
     5281 atgcagccga tatcagcatt ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct
     5341 ggttgcaaga taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt
     5401 ctcactggtt tgacgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc
     5461 atcagtcaga cgattacccg cataacccca acactgagga cttaagccaa ttctcttata
     5521 cggtgagaaa taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct
     5581 acaaactgac ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta
     5641 aagagatgca aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgaag
     5701 taaatcagga gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc
     5761 ttcatgcggc ggaattttcc gccgccttat tgagcgagat agcgatgcac gttacagcca
     5821 agccctccag ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt
     5881 cgcagtttac tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat
     5941 atatcgcagc gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg
     6001 ctggcctgga ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac
     6061 gtatttttaa tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcttttc
     6121 tcaaagaaca tgagtttctg gttggtatct cgatcgatgg cccccaggag ctacatgacc
     6181 gttacagacg cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc
     6241 gtctgaaatc atatcaagtt gagtttaata cgttaaccgt cattaataac gttaatgtcc
     6301 attaccctct tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta
     6361 tcgaattgct cgaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat
     6421 tccgtatcat tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt
     6481 ttatgcaatg ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg
     6541 tcagccgttt tttggggaat gggcatacca gttgtatttt ccaggagtcc tgcaaggata
     6601 atctggttgt tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt
     6661 acaaaattgg aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag
     6721 cgcaaaaaaa acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg
     6781 gcggttgtcc taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg
     6841 aaggttataa aaaccttttt tcaaccatgg taccttatat gaacgccatg gtggagttag
     6901 ctaagaacag agtaccgctt tatcacatta tggatgttgc aaaacaaatg gagaataatt
     6961 aataacacgc ccttttagct ccattcttag cctccttctt atgaccttaa caaggcaccc
     7021 gacataaatt cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt
     7081 acgtggtatg cgtcagggca ttagcaatac ccttgattat caatagcata accataacta
     7141 atgaaaatga aatatttctt tatatttcat attgttaagt gatacttaaa taaatttcat
     7201 cagtaaatca ttgccataga taatcattgg atggcacaat caattttcgc taatgataac
     7261 cattcccatt acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg
     7321 gctgcgtaag aataataaaa cttcagtgtt gttaatcatt attatcctcg ccatgatcct
     7381 tggcgtggtt aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct
     7441 aagccagaaa gaaaccgata agctttggca gcttgtcctt tggttccctg ccctgctggg
     7501 gatctttgta ttaatatccg taaacaaaac atggttaatt aaactactga ctattcgctg
     7561 gcgcgaatgg ttaacagatt actaccttaa tcggtggttt gcagataaaa actactactt
     7621 cacgcagatc tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga
     7681 cattctctta ctgatcagca aaacgctcag cctttcattt ggcttcatcc agtcacttag
     7741 catgctgatc acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt
     7801 tggtggaaca gaatggaata tccaggggta tatggtctat accgttgtgc tcattgtgat
     7861 cggtggaact ttatttaccc ataaggttgg taaacgtatt cgtccgctta atgtggaaaa
     7921 acaacgtagc gaagcgacat ttcgaacaaa tcttgtgcag cataataagc aggcagaact
     7981 gattgcgcta agcaatgctg agtctctaca acgccaggag ttgagcgaca attttcatac
     8041 cattaaagag aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcagaa
     8101 tatttattcg cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag
     8161 cgggcagatc aatctgggcg gactgatgaa atcgcgccag gcatttatgc tggtatcgaa
     8221 caatttaagc tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga
     8281 tcgtttgtat gagttccatc aactcaccga acagcgccct acgaataaac ctaaaaattg
     8341 ccaacatgcg gtacaagtgg ctgacgcgag tattcgtact cctgataata agatcatatt
     8401 agagaacctg aactttcatg tttcgccagg caaatggcta ttactgaaag gttactctgg
     8461 tgcgggaaaa accacactgc ttaaaacatt atctcactgc tggccgtggt ttaaaggtga
     8521 tatttcttct cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaaccggctt
     8581 actgaaagag attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga
     8641 agtactgcat caggttggtc tggggaaatt ggctgcgcgt attcatgacc acgatcgctg
     8701 gggagatatt ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg
     8761 acgtccgaaa tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat
     8821 ccgcttactg cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca
     8881 tcaatccggt gtctggaacc tggcagatga tatttgtgac attagcgcgg ttttataaaa
     8941 acaataaaag ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg
     9001 tcattttatg tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgcatg
     9061 tttttgaaga gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt
     9121 acaccagtga aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga
     9181 gaaccaaccc tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata
     9241 ttcgcccgga gaaaatctct attcacggtg cgtcgcccta tcagaatgcc tatttgattg
     9301 acggtattag tgccactaat aacttgaacc cagcgaatga gtccgatgcc agtagtgcaa
     9361 ccaatattag cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga
     9421 cgctttatga cagtgttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg
     9481 caaagatcaa acgcttcaac gctgatgata gcaaggtgaa attgggttat cgcactacgc
     9541 gttcggactg gttaacatcg catatcgatg agaataacaa gagcgcattt aatcaaggct
     9601 cttcaggaag tacttattac tccccagatt ttaaaaagaa cttttatacc ttgtcgttta
     9661 atcaggaact cgctgataac ttcggcgtta ccgccggttt atcgcgccgc cagtctgata
     9721 tcacccgcgc ggattatgtt tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa
     9781 acgttatcga tactgcattg agcaaattta cctggtttgc cagcgaccgc tttacccacg
     9841 atttaacctt aaaatatacc ggctccagcc gtgattataa taccagcacc ttcccgcagt
     9901 ctgatcgcga aatgggtaat aaatcctacg gtctggcatg ggatatggat acgcagctcg
     9961 catgggccaa actgcgtacc accgttggtt gggatcatat tagtgattat acccgtcacg
    10021 atcatgacat ctggtacacc gaactttcat gtacatatgg tgatattaca gggcgttgta
    10081 cccgtggcgg attaggacac atttcccagg ctgtagataa ttacaccttc aaaacacgcc
    10141 tggactggca aaaattcgcc gtgggtgatg tttcgcatca accctacttc ggcgcggaat
    10201 acatctattc cgatgcatgg actgaacgcc ataaccagtc tgaatcctat gtgattaatg
    10261 ctgcaggaaa gaaaactaac cataccattt accataaagg taaaggcagc ctgggaattg
    10321 acaactacac gctgtatatg gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg
    10381 gtgtgcgtta tgactatgac aactatctgt caaaccacaa tatctccccg cgctttatga
    10441 cggaatggga tatttttgct gatcaaacct caatgattac agcaggttat aaccgttact
    10501 atggcgggaa tattcttgat atgggattac gtgatatccg caatagctgg acggaatcgg
    10561 tatcaggcaa taaaaccctg acgcgttatc aggatttgaa aacgccttat aacgatgaac
    10621 tggcaatggg attgcagcag aaaatcggta agaacgttat tgcgcgcgca aactatgttt
    10681 accgtgaagc gcatgatcaa atcagcaaaa gcagtcgtac cgacagcgcg actaaaacca
    10741 ccattactga atataacaac gacggcaaaa caaaaacgca ttcgttcaac ctcagttttg
    10801 aactggccga acccctgcat atcagccagg tagatattaa cccgcaaatt gtctttagct
    10861 atatcaagag caagggcaac ttgtcgttaa acaatggtta tgaggagagc aataccggtg
    10921 ataaccaggt ggtttataac ggtaatctgg tttcttacga tagcgttcca gtggcagatt
    10981 ttaataaccc attaaagatc tccttaaaca tggatttcac gcatcaaccg agcgggttag
    11041 tgtgggcgaa tacgctggcc tggcaagaag cgcgtaaagc tcgcattatc ctgggtaaga
    11101 caaatgcgca atacatcagc gaatattcag attacaagca gtatgttgac gaaaaactgg
    11161 atagcagcct gacctgggac acccgcttgt cctggacgcc acaatttctg aaacaacaaa
    11221 acctgacgat cagtgccgat attctcaatg tattggatag caaaaccgct gttgatacaa
    11281 cgaataccgg tgtggcgacc tacgccagtg gccgtacttt ctggcttgat gtcagcatga
    11341 aattttaata gttcggattt tacttaaccg ggtaaattcg cccggttttc gcatggagat
    11401 aattatgaga aacctctgtt tcttactgac gttagtggca actctgttgc tccccgggcg
    11461 gctgattgcc gccgccttac cgcaggatga aaagttaatt accgggcaac tgggcaatgg
    11521 cttgcgatat atgatttatc cgcatgctca tccaaaagat caggtaaatt tatggctgca
    11581 aattcatacc ggttcattgc aggaagaaga caatgagcgc ggcgtggctc attttgtaga
    11641 acatatgatg tttaacggca caaaaacatg gccgggtaat aaagtcatcg aaacatttga
    11701 gtcaatgggc ctgcgttttg gtcgcgatgt taatgcctat accagctatg acgaaacggt
    11761 gtatcaggtg agtttgccga ctacgcagaa acaaaatctg caacaagtga tggcaatctt
    11821 cagtgaatgg agtaatgccg caacctttga aaaactcgaa gtagacgctg aacgtggcgt
    11881 aattactgag gaatggcgtg cccatcagga tgcgaaatgg cgcacctctc aggcgcgccg
    11941 ccctttcctg ctggctaata cccgtaattt agaccgtgaa cctatcggcc tgatggatac
    12001 tgtcgccacg gtcacaccgg cacaattgcg ccaattttat caacgctggt atcaaccaaa
    12061 taatatgacc tttatcgtgg ttggcgatat cgacagtaaa gaagcgctgg cgctgataaa
    12121 ggataattta agtaagcttc cggctaacaa agcagctgaa aatcgcgtct ggccgacaaa
    12181 agccgaaaac cacctgcgct ttaatatcat taatgataaa gaaaaccggg tgaacggcat
    12241 cgcactctat tatcgcctgc caatggtaca agtgcacgat gagcaaagct ttatcgaaca
    12301 agctgaatgg agcatgttag ttcagctgtt caatcaacgt ctgcaggaac gcatacagtc
    12361 gggcgagttg aagactattt ctggcggcac tgcgcgcagc gttaaaattg cacccgatta
    12421 tcagtcgctg tttttccgtg taaatgcacg agacgataat atgcaggatg ctgcgaatgc
    12481 attaatggca gagttggcaa ctattgatca gcatggtttt tctgctgaag aactcgatga
    12541 tgttaaatct acccgcctca cctggctgaa aaatgcggtt gatcagcaag ctgaacgtga
    12601 tttacgtatg ctgaccagtc gcctggcatc cagctcatta aataatacgc cgttcttgtc
    12661 gccggaagag acatatcaac tttcgaaacg tctgtggcag caaattaccg tgcaaagtct
    12721 ggcggaaaaa tggcagcagt taagaaagaa ccaggacgca ttttgggagc aaatggtaaa
    12781 caatgagctt gccgccaaaa aagcattgtc tcctgcggct atcctggcgc tggaaaaaga
    12841 gtacgccaac aaaaagctgg cggcttacat cttcccaggc agaaatttat cgttaacagt
    12901 agacgctgac ccacaggcgg aaattagcag caaagaaacg ctggctgaga atctgacatc
    12961 attaacactt tccaatggtg ccagggttat tctggcaaaa tccgcgggtg aagagcaaaa
    13021 gctacaaatt actgccgtat cgaataaagg cgatttaagt ttccctgcgc agcaaaaatc
    13081 acttatcgcg ctggcaaata aagcagtaag cggaagcggc gttggtgagc tctcctcttc
    13141 cagcctgaaa cgctggagtg cggaaaattc ggtaaccatg agcagtaaag tcagtggcat
    13201 gaatacgttg ctctccgtta gcgcgcggac taataaccct gaacctggtt ttcagttgat
    13261 taaccagcga atcacccaca gcacgattaa cgacaatatt tgggcatcgc tacaaaatgc
    13321 tcaaattcag gcgttgaaaa cgctcgacca gcgtccagcg gagaaattcg cccagcagat
    13381 gtatgagacg cgctatgctg atgaccgcac gaaattactg caagaaaatc agattgtaca
    13441 gtttactgcc gcagatgcgc tggctgccga tcgccagttg ttttcatctc cagcggatat
    13501 cacgtttgtc attgtcggta atgtctcaga agacaaactc gtggcgttaa ttacgcgtta
    13561 cttaggatca atcaaacact ctgattcgcc attagccgca ggtaaaccat taactcgcgc
    13621 gacggacaac gcatcggtta ctgtaaaaga acaaaatgaa cctgtggcac aggtttcaca
    13681 gtggaagcgt tatgattccc ggacacctgt taatctggcg acgcgtatgg cgctcgatgc
    13741 ttttaacgtc gcactggcaa aagatctacg tgttaatatt cgtgaacagg catctggagc
    13801 atacagcgtt tcttctcgcc tctcggttga tcctcaggcc aaagatatca gtcatttgct
    13861 ggcttttact tgtcaaccag aacgacatga tgaactgtta acgttagcga atgaagtgat
    13921 ggttaagcgt ctggctaaag ggatcagtga gcaagaactg aatgaatacc agcaaaacgt
    13981 tcagcgcagc ctcgatatcc aacagcgtag cgttcaacaa ttagcgaaca ctattgtaaa
    14041 tagtcttatt caatatgacg atcctgcagc atggactgag caggagcaat tgttgaaaca
    14101 aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc cggtaaatac
    14161 ttataccgga gtattattgc caaaataata acagccc
//
LOCUS       DAZKGS010000118        14002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKGS010000118 DAZKGS010000000
VERSION     DAZKGS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..544)
                     /gene="nlpC"
                     /locus_tag="ACHOQY_003458"
     CDS             complement(80..544)
                     /gene="nlpC"
                     /locus_tag="ACHOQY_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9679499.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(622..1371)
                     /gene="btuD"
                     /locus_tag="ACHOQY_003459"
     CDS             complement(622..1371)
                     /gene="btuD"
                     /locus_tag="ACHOQY_003459"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9679500.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1371..1922)
                     /gene="btuE"
                     /locus_tag="ACHOQY_003460"
     CDS             complement(1371..1922)
                     /gene="btuE"
                     /locus_tag="ACHOQY_003460"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9679501.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(1985..2965)
                     /gene="btuC"
                     /locus_tag="ACHOQY_003461"
     CDS             complement(1985..2965)
                     /gene="btuC"
                     /locus_tag="ACHOQY_003461"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9679502.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(3066..3365)
                     /gene="ihfA"
                     /locus_tag="ACHOQY_003462"
     CDS             complement(3066..3365)
                     /gene="ihfA"
                     /locus_tag="ACHOQY_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9679503.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3370..5757)
                     /gene="pheT"
                     /locus_tag="ACHOQY_003463"
     CDS             complement(3370..5757)
                     /gene="pheT"
                     /locus_tag="ACHOQY_003463"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9679504.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKVMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5772..6755)
                     /gene="pheS"
                     /locus_tag="ACHOQY_003464"
     CDS             complement(5772..6755)
                     /gene="pheS"
                     /locus_tag="ACHOQY_003464"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9679505.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6965..7089)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(7039..7083)
                     /gene="pheM"
                     /locus_tag="ACHOQY_003465"
     CDS             complement(7039..7083)
                     /gene="pheM"
                     /locus_tag="ACHOQY_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9679506.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(7206..7562)
                     /gene="rplT"
                     /locus_tag="ACHOQY_003466"
     CDS             complement(7206..7562)
                     /gene="rplT"
                     /locus_tag="ACHOQY_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9679507.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7615..7812)
                     /gene="rpmI"
                     /locus_tag="ACHOQY_003467"
     CDS             complement(7615..7812)
                     /gene="rpmI"
                     /locus_tag="ACHOQY_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9679508.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7909..8451)
                     /gene="infC"
                     /locus_tag="ACHOQY_003468"
     CDS             complement(7909..8451)
                     /gene="infC"
                     /locus_tag="ACHOQY_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9679509.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8455..10383)
                     /gene="thrS"
                     /locus_tag="ACHOQY_003469"
     CDS             complement(8455..10383)
                     /gene="thrS"
                     /locus_tag="ACHOQY_003469"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9679510.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10907..12806
                     /gene="espL1"
                     /locus_tag="ACHOQY_003470"
                     /pseudo
     CDS             10907..12806
                     /gene="espL1"
                     /locus_tag="ACHOQY_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12978..13085
                     /gene="yniD"
                     /locus_tag="ACHOQY_003471"
     CDS             12978..13085
                     /gene="yniD"
                     /locus_tag="ACHOQY_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9679511.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13138..13896)
                     /gene="ydiY"
                     /locus_tag="ACHOQY_003472"
     CDS             complement(13138..13896)
                     /gene="ydiY"
                     /locus_tag="ACHOQY_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9679512.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         3600 a         3647 c         3175 g         3580 t
ORIGIN      
        1 gaatttttgt ctgcaaaata ctactgtatt caggagtaaa tggacggcga tgtatcatcg
       61 ccgtccattt ttcgacatac taaattcgtc tcgcctgcca gaaatttttc tgccaataga
      121 cattatcaag tgaggaacgc atcactccct tgctggtaga ggcgtggata aattggttgt
      181 tggtatcgta aatacctaca tgcaaaccat tttgtccgga acccgttttg aaaaagacca
      241 ggtcaccagg cagcaactcg tctttatcaa tttgcgtgcc gatagaggct tgttctttgg
      301 tttctcgggg cagctgcaaa tcgaaacgat cgcgcatcgt tacaaccaca aatcccgaac
      361 agtccacacc gcgccgcgtc atgccaccat aacgatacgg cgtgccatgc cagctttgta
      421 gctggtcgtt caaaccggca ataacggtaa tcgaatcaga aagtctggca tttggcggcg
      481 gtgctttatg gtggctacac ccggccagaa acagtgctgt gatcaaaata aggcagaaac
      541 gcattccgta cggttcctct gttttttatt cttgcattaa tttagcgtcg taattacccg
      601 attttcaaga tactaatgaa atcagatggt cgaaatcagc attctgtgac cttcgatatc
      661 cagacggcga aaattcatcc cataggcctg cgccagattt ggcggcgtga gcacctcttc
      721 tctgcgtcca ctggccagca tttttccacc ttttagcaac cacgcccgat gcgcatgacg
      781 caatgtgtgg ttgagatcgt gactgctcat cacaatcgcc agtccttgct gacacagagc
      841 gctcagaatt ttgtctaacg cactttgttg cgcaacatca agactgttca tcggctcatc
      901 aagaagcagc aattgacctg cgggattggc ttgtggtgtg atttgcagca ccaccgcagc
      961 aagacgtacg cgttgccatt caccgccgga aagttgattg gtgctacgtc cgagtttgtc
     1021 atcaagagcc agcgcccctg cgacatcatt cagtagttcg gtacgcgttt tatcgtgctg
     1081 atgcagtgtc aggtagtgcc agaccggcat tgcaaacggc ggcgtctgct gttgtgaaag
     1141 gtaggcgcga tgcagcgcga gttttgttgc ggaccatgct tccagtggtt gccccgcgaa
     1201 ctgaatgctt cccttaccgc tggtcattcc ggccattcgt gcaagcaagg tactcttacc
     1261 cgcgccattc ggccccacca ggtgcaggat ctccccagcc cgaacctcgc cagaaagcgg
     1321 ccccaggcgg gtagtttccg caacatcctg taactgcatc acaatagaca ttattttgcc
     1381 aacgccagtt taatgctttc catcacaatg ggatcttcag gcgtcatatc cggggaaaaa
     1441 cgctggatga ccaacccgtc cctgccaact aaaaatttct caaaattcca taaaatatca
     1501 tcagggtaca gcggtgcacg acctttgctg accatacggg catagaatcc gctctcttcc
     1561 ggcgcgactg cggtcggcgc tgcggcaatc aatttttgat acagcggatg gcgtccttcg
     1621 ccattaactt caatcttact gaacatcggg aacgtcaccc cccatgtggt ggtacagtaa
     1681 gttttaatct cttcatcgct gcccggttct tgttccagaa actggttgca cgggaatccc
     1741 agcaccacaa aacctcgatc ggcccaggct ttctgaatat tctccaactg ctcatattgc
     1801 ggcgttaagc cacactttga ggcgacattg acaatcaaca gcacattacc agcgtacttc
     1861 tccagcgtgg tcacttcacc gtcgatatct ttcactacgg tcgtcagaat ggaatcttgc
     1921 atcgtttctc ctgggtgtgg tcagtaaaaa tcttagcttt taatcataga ccgtcttttt
     1981 gcggctaacg tcttgctttt aacaataacc agataaacac cggcgcaccc aacgttgcgg
     2041 tgaccacgcc aataggcagc tctgcggcag ctaatgccag gcgcgctaca atatcggcca
     2101 gcagcaatgc gctcgcccct gccagcgcgc agccgggaag taatacgcga tgatcggtta
     2161 aaccacacaa ccgaagaata tgtgggatca ccagaccaat aaagccgata gcacccgcca
     2221 gcgccacact gacgccaacc atccagccgg tcgcggccac cagcacattg cgccagaacc
     2281 acaggggtaa acccagttgc cgcgccgaga tctcgccaag tgctaacata ttcatcggcc
     2341 tggactgaca acagatccac aacaacacgg ggatcaatgc cagcatcagc cagctttgcc
     2401 gccagtctac gccgccaaaa ccgcccatca tccagtacat cagctgacgt aaatcaacag
     2461 aggtggaaaa gtagatagcc cacgtcatta gtgcgctaca gataatccct aatgcaacgc
     2521 cagccagcaa taaccgactg gtcgaaagat gacgacgggc gaaacgtaag agtattaaag
     2581 tgatgataag cgcgccagca atcgcacaca gccctagcgc ccagttgggg agttgccctt
     2641 gcccaagcaa taccgcggcg ataagcccca cgcctgcgcc gttagagacg ccaagtagtc
     2701 caggttctgc cagaggattt tcaaacaacg cctgcattac agcgccggat atagccagcg
     2761 ccgcaccaac cagcaataca gccagcgtac gtggcaggcg aatttgccag acgaacagtt
     2821 cgccacgagg agtaaaccag tcacctggcg agatccattg ttcaccggcg caaaggctta
     2881 agagaagcgc cagcagcatc aaaactgaca ggcataataa ccagcgaata ttttgtcgct
     2941 gttgttggcg ggcaagtgtc agcatggtat ccgttctgct gaagtgtcat ggcgttgatt
     3001 ttacggtgac tcttcgacag tgaaaagaaa aaaggccgca gagcggcctt tttagttaga
     3061 tcagattact cgtctttggg cgaagcgttt tcgacccggc tttttaactt ctgcccgggt
     3121 ctgaaggtca ccacgcgccg tgctgtaatg ggaatatcct cgcccgtttt cgggttacgt
     3181 cccgggcgtt gattcttatc acgcagatcg aagttaccaa aaccagagag tttcacctgt
     3241 tcgccgtttt ccagagcgcg acggatctct tcgaaaaaca gttcaaccag ttctttggca
     3301 tcccgcttgc taagcccaag cttatcaaac agatattctg acatttcagc ttttgtaagc
     3361 gccataggtt caatccctca atgatgcctg gaatcgctct tttaatgcct ctacacattt
     3421 ggcgacggta gcggcaatct cctcttcttc gagtgtacgg ctggtatctt gcaggatcag
     3481 gcttatggcg aggctcttat acccctccgc aacaccctta ccgcggtaca cgtcaaataa
     3541 gtttacgcca actacctgat ttacgccaac tttcttacat tcggataaaa tatccgctgc
     3601 gggaacgttt tctgcgacca ccaccgcgat gtcacgacgg ttcgccggga agcgagaaat
     3661 ctcgcgcgcc tgaggcacca cgcggtctgc gagcttgttc cactccagtt cgaacaccag
     3721 agtgcgaccg ttaagatcca gtttacgttc cagttcagga tgaacaaccc caacaaaacc
     3781 aatacgttca cctttcagat aaatcgctgc ggattgcccc ggatgcagtg ccggattcgc
     3841 ttctgcacgg aactcaacct cattcagttt accggtcagg tcgagaacgg attcaagatc
     3901 gcctttcaaa tcatagaaat caacggtctc ttttgccagg ttccagtgct cttcgtaacg
     3961 gttaccgcaa atcacaccgg ctaacatcag atcctgacga atgcccaacg gtgcctgagt
     4021 atctggtacg aaacgcagac cgctttcgaa aatgcgcaca cggttctgct gacggttctg
     4081 gttgtacacc acggttgcca gcaggccggt ccacagagaa agacgcattg ctgacatttc
     4141 aacagagatc gggcttggca gcagtaaggc ttcaacgcct ggatggatca tctgctgcac
     4201 tttcggatca acgaagctgt aggtgatcac ttcctgatag cctttgtcgt tgagcagcgt
     4261 tttcacgcgc ttgagcgaca ggtcagcttc acggtgagta cccataatca ggcttgcctg
     4321 taccggctca tccgggatgt tgttgtagcc gtaaacacgc gcgacttctt cgaccagatc
     4381 ttcttcaatc tccatatcga aacgccagct cggcgcaact gcctgccact cgtctttgcc
     4441 ttcggtcact tcgcagccga gacgacgcag aatgtcagtt acctgctcat ccgcaatatg
     4501 atggccgatc aggcgatcca gtttgctacg acgtaaagtg atggttgcac gcttcggcag
     4561 cgttgcttcg ttggtgatat caattaccgg accagcctca ccaccgcaga tgtcgatcag
     4621 cagacgggtc gcacgttcca tcactttgtg ctgcagtgcc ggatcaacgc cacgctcata
     4681 acggtgagac gcatcagtat gcaggccatg acgacgagca cgaccggtga tagacagcgg
     4741 gctgaagaaa gcgcattcca gcaagacgtt ttgcgtttcg tcattcacgc cagagtgttc
     4801 gccaccaaag atgccgccca tcgccagcgc cttgttgtgg tcggcgatga ccagagtgtc
     4861 agcattcagc ttcgcttcag tgccatcaag cagtaccaac gtttcgccct ctttcgccat
     4921 ccgcaccaca atgccgcctt caatgcgatc tttatcgaaa gcgtgcatcg gctggcccag
     4981 ttcgagcagt acatagttgg tgacgtcaac aactgcatcg atagaacgga tcccgcaacg
     5041 acgcagtttt tctttcatcc acagcggagt tggcgcttta acgttaatgc cttttaccac
     5101 acggccaaga taacgcgggc aggcttccgg cgcttcgact gtaatcggca gcgtgtcgtc
     5161 gatggtcgca ccaaccggaa cgatttccgg ttcaaccagc ggcagctgat tcagcacggc
     5221 aacgtcacgc gcaacaccaa tgatacctaa acagtcggca cggtttggcg tcacgctgat
     5281 ttcgatggtg ttgtcatcaa gtttcaggta ttcacggatg tcggtgccaa tcggcgcatc
     5341 cgcaggcagc tcgataatgc cgctatgatc gtcagaaatt cccagctcag aaaaggagca
     5401 cagcatccct tcagacggtt caccacgcag tttcgccgct ttaattttga aatcacccgg
     5461 cagaacagca ccaatggtag ctaccgccac acgcaggccc tgacggcagt ttggcgcacc
     5521 gcagacgatg tccagcaggc gatcgccgcc aacattcact tttgtcacac gcagtttgtc
     5581 agcgttcgga tgctgcgcac actcaaccac ttcaccaacg accacgccgt ggaagctacc
     5641 ggcaaccggt tctacaccgt caacttccag gcccgccata gtgatttgat tcgccagcgc
     5701 atcgctatca atcgccgggt tcacccattc gcgtaaccac agttcactga atttcataat
     5761 ctattcctgc cttatttaaa ctgtttgagg aaacgcagat cattttcgaa gaatgaacgc
     5821 aggtcggtga cgccgtaacg caacatagtc agacgctcca tccccatccc gaaggcgaaa
     5881 ccagagtaaa cttccgggtc gatgccaacg ttacgcagca cgttcggatg caccatcccg
     5941 caacccagca cttccagcca tttaccgttt ttacccatga cgtcaacttc tgcagaaggc
     6001 tcggtgaacg ggaagtagga aggacggaag cgaatctgca aatcttcctc aaagaagtta
     6061 cgcaggaaat cgtgcagagt gcctttcagg ttggtaaagc tgatgttggt atcaacaatc
     6121 agaccttcca tctgatggaa catcggcgtg tgagtctggt cgtagtcgtt acgataaacg
     6181 cggccaggcg caatgatacg aatcggtggc tgctgggctt tcatggtgcg gatctgtacg
     6241 ccagaggtct gggtacgcag caggcgggta gtgtcaaacc agaaagtgtc gtggtcagcg
     6301 cgcgccgggt ggtgaccagg aatgttcaga gcatcgaagt tatgataatc gtcttcgatt
     6361 tccggcccgg ttgccacggt aaagccaagc tcaccgaaga aactttcgat acggtcgata
     6421 gtacgggtaa ccggatgcag accgccgttt tcaatgcgac gacctggcag agagacatca
     6481 atcgtttccg ccgccagacg cgcattcagt gcagcgcttt ccagttccgc tttacgcgca
     6541 ttcagcgcct gctgaacctg ctctttcgct tcgttgataa ccgcaccagc tgccggacgc
     6601 tcttctggcg gcagctcacg cagggtcgtc atctgaaggg ttaagtgccc ttttttaccc
     6661 aaatattcga cgcgcacatt atctaacgcg gcaacatctg acgcctggct aatggccgcc
     6721 ttcgcactgg caaccagttc tgcgagatgt gacatggttt tcctcattgt gtcagtggtg
     6781 acactggttc gttggactta gagcctatcc catcaggcta ttttacttgc cattttggtc
     6841 ccgggcagtg ctcgaaattc tcacgtactt aaatacgctc cggtttctcc gcgctggccg
     6901 tgtccagact ggctgcgaca attacacctg atgagacagg ctttttattt ttcaaaacgc
     6961 gcatacaaaa aaagcctcca ctgggaggct ttcaggcgct gttttccgtt tctcttctca
     7021 cgcgctagcc tcctggattc aggtgctaaa gtaaaaaaag aagcggaaaa tagcagcatt
     7081 cattgcttgc gttacctttt ggtactcttc aaaagacctt tattgaaaag gctacggcga
     7141 taaaagtcaa tgttttgatg gcgttgaaac gaaaagaggg agactagctc cctctttcaa
     7201 ctggcttatg ccagagctgc tttcgctttt tcaaccagag cggtgaacgc tactttgtcg
     7261 aatactgcga tatcagccag gatcttacgg tcgatttcaa cagaggcttt tttcaggcca
     7321 ttgatgaatt tgctgtaaga aataccgttc tgacgtgctg ctgcgttgat acgcgcaatc
     7381 cacagttgac ggaactgacg cttacgttga cgacggtcac ggtaagcata ctgaccagct
     7441 ttgataacag cctggaaggc aacgcggtat acgcgagaac gcgcaccgta gtagccttta
     7501 gcttgtttca aaattttctt gtgacgtgcg cgtgcaataa caccacgttt tacgcgagcc
     7561 atatgtgctc tcctgtatct atattctaat taaaaagtta aaaacgttaa cggcttatgc
     7621 gtacggcagg cacgcgatta ccaggcccag atcgcctttg gaaaccatgg ctttcggacg
     7681 caggtgacgt ttacgtttgg tcgctttttt ggtcagaatg tgacgcaggt tagcgtgctt
     7741 gtgcttaaaa ccacctttac cggttttttt gaagcgctta gcagcaccgc gtacggtctt
     7801 aatttttggc attttaataa cttccacttc gcattgttaa taaacgaaac aaaggcgaac
     7861 aaagcctgtg aagcccgaag gctccacaga cagtgctact tgaaggcctt actgtttctt
     7921 cttaggagcg agcaccatga tcatctgacg gccttcgatc ttcgttggga aggattcgac
     7981 cactgccagt tcttgcaaat cgtctttcac gcgattaagc acttccatac cgatttgctg
     8041 gtgcgccatc tcacgaccgc ggaaacgcag cgtgattttg gctttatcac cctcttcgag
     8101 aaagcgaatc aggctgcgga gttttacctg atagtcgcct tcatctgtac caggacggaa
     8161 tttaatttcc ttaacctgga taactttttg ctttttcttc tgttccttag aagacttgct
     8221 cttttcatag aggaatttgc cgtaatccat tatacgacaa accggcggct cggcgttagg
     8281 gctgatctcg actaagtcta ctccggcttc ttctgctttc tccagagctt ctctcagact
     8341 cacaatacca agctgctcgc cttccagacc tgttaagcga acttcctggg cgcgaatttc
     8401 gccattgata cggttagggc gcgccgtttg aactcgtttt ccgcctttaa taccttattc
     8461 ctccaattgt ttaagactgc ggctgcgaat ctcttgttgc agcttctcga tcacttcatt
     8521 tacgtccatg cttcccaggt ctttaccacg gcgggtgcga acggcaactt tgcctgattc
     8581 cacctcttta tcaccacaga ccagcatata tgggacgcga cgcaaagtgt gctcgcggat
     8641 tttaaagcca atcttctcat ttctcaagtc tgctttaaca cgaatgcccg catttgatag
     8701 tttttgcgtc aattcgttaa cgtattcaga ctgtgaatcg gtaatattca tgataacaac
     8761 ctgaactggc gcaagccagg tcgggaagaa accagcgaat tcttcggtca ggataccgat
     8821 gaaacgttcc atcgatccca gaattgcgcg gtgaatcatt accggtactt tacgttcatt
     8881 gtcttcgcct acataagaag cgctcagacg agaaggcaaa gagaagtcca gctgtactgt
     8941 accgcactgc catgcacgat cgaggcagtc atacagggta aattcaattt tcggaccgta
     9001 gaaagcgcct tcacccagtt gatattcaaa cgggatgttg ttttcttcca gcgcaaccgc
     9061 caggtccgcc tcagcacgat cccacatttc atcactgcca atacgttttt caggacgagt
     9121 ggagagtttg acgacgatct tctcgaagcc aaaagtgctg tacatatcat agactaaacg
     9181 gatacatccg ttaacttcat cgcgaatttg ttcttcagta cagaagatat gcgcgtcatc
     9241 ctgggtaaat ccacgcacgc gcatcaggcc atgcagcgaa cctgacggct cattacggtg
     9301 gcagctacca aactcggcca tacgcagcgg cagatcgcga taagacttca gcccctggtt
     9361 gaaaatttgt acgtgacccg ggcagttcat cggcttaatg cagtattcac ggttctcaga
     9421 agacgtggtg aacattgcat ctttgtagtt gtcccagtga ccggtttttt cccacaggac
     9481 acggtccatc atgaacggac ctttaacttc ctgatactgg tactctttca gtttagaacg
     9541 aacaaacact tctagttcac ggaagatggt ccagccgtcg ttgtgccaga ataccatacc
     9601 cggcgcttct tcctgcatat ggtacaggtc gagctgttta ccgattttac ggtggtcgcg
     9661 tttcgcggct tcttccaggc gctgcaggta agcgttaagc gcttttttgt ctgcccacgc
     9721 cgtaccgtaa atacgttgca acattttgtt gttgctgtcg ccacgccagt aagcccctgc
     9781 cgttttcatt agtttgaaat gatggcagaa acgcatgttc ggtacgtgcg gaccgcggca
     9841 catatcgaca tattcttcat ggaagtacag acctggcttg tcatcatggg cgatgttttc
     9901 gtcaagaatg gagactttgt agctctcccc acggttggcg aaagtttcac gcgcttcgtg
     9961 ccagctgact ttcttcttaa tgacgtcgta gtttttctca gcaagctcat gcatccgctt
    10021 ctcgagtgct tcgacatctt cctgggttaa cgtgcggtca agatcaacgt cgtaataaaa
    10081 accgttgtca ataaccgggc cgattgccat tttggtatgc ggccaaagtt gtttaatcgc
    10141 gtgccctaac aggtgcgcac aggagtgacg aatgatctcc agaccgtctt cgtctttggc
    10201 ggtaatgatg gacagttgtg cgtcgttttc aatcagatcg caagcatcaa ccagttcgcc
    10261 attaacgcgc cctgcgatac aggctttcgc cagacctgga ccaatgtcca gcgcaacatc
    10321 catggggctt acagcgtggt cgtaatggcg ttggctgcca tcaggaagag ttataacagg
    10381 cattttatat ccttatttgc agtggtgacc cacacgaaag atcacataca aagaaaaatt
    10441 tgtttattaa cagttcattg cgaaaccatc tagccaacaa atgctaaatt ggttcgcaat
    10501 caggtacaca aatgaaagac attaacctct caccttccac ccggttgagc gatgttaaca
    10561 ctaaaaaaag ggagattgta cctttccgtt tcacattgat tcgattcgca attcgtttgt
    10621 agtgagtaca ccagcaaaac gcttaaagaa ccactgataa catcacggtg cgtctgaaag
    10681 gtttactgcg agcggattta cagcagcata aactgaatcc agcaaagttc gttgcgtgat
    10741 ttggcggtag tattttaaac gctcatgaga ttaatgacga agtggtcata tcacaatgat
    10801 aaaagtgaca caattcttat aacaattttt cgtgcacatt tcgttctggc gataataatt
    10861 aatcatcatc ctcaaaacct ccagatatat ataaggcgaa tagattatga gtcaaaacga
    10921 tatcattatc agaactcatt ataagtctcc tcatagattg cacatcgata gcgacatacc
    10981 aacaccttca tcagagccta ttaataaatt tgcgcgccag ctcatcaccc tacttgatac
    11041 ctctgactta agttcgatgc tgtcatactg tgttactcag gaatttaccg caaactgtcg
    11101 aaaaatatca caaaattgtt attccactgc cctttttacc attaaccttg ccacttcacc
    11161 catccatgca gaaaatatac tcattacatt acactataaa aaagatatca tttccttatt
    11221 actggaaacc acgcctatta aagctaacca tttgcgaagc atactggatt atattgaaca
    11281 ggaacagtta actgctgaag atcgtaacca ttgtatgaaa ctgtctaaaa aaatccatag
    11341 agaaaaaact atacacccaa cagtaaatct caatggtagt gcatgttttt tgcaatctcc
    11401 ttctgacgct attttttgtc gccatctgtc attgcaatac gcccttgatt cattgagaaa
    11461 tggaaaaggc aaagtcaacc tgattaaaca ttactcctcc gttgaatcca tacagcagca
    11521 tgtcccctta gtccgggacg cggagttcag agcattactt cgccatcctc ctgcagggag
    11581 tcgcgttatc gcgagtaagg attttggctt cgctttagat tttttctttt gtcgaatgat
    11641 ggcaaacaat atcagtcata tgtccgcgat tttatatata gacaatcata ctttgtcagt
    11701 aaggctacga ataaagcagt cagtgtatgg acaattaaac tatgttgtgt ccgtttacga
    11761 cccgaacgat accaacgttg ccgtcagaga cacccacagg acagcacggg gctttctttc
    11821 gcttgataag ttcatcagtt caggtcccga tgctcagacc tgggctgata ggtatgttcg
    11881 caactgtgca attgctattc tgcccctatt acctgtggga gttccagggg ctattttcgc
    11941 gggtattgca tcacgaatgc catttgcccc tatacatcca tcggcaatgt tgttaataat
    12001 ggctacaggc cagactcaac agcttattac attattcaaa cagttgccca tactccctga
    12061 aaaagaaatc attgaaataa taactgcgca gaatagcgtt ggtacacctg ctttattttt
    12121 ggctatgatg aacggacata ctgacaacgt gaaaatattt atgcaagaaa ttcagtcact
    12181 ggtagataat cacatcattc atgaagataa cctggttaaa ttactgcaaa ctaaaagtgc
    12241 taacgaaaca cctggactct atatctccat gttgtatgga ttcgatgaaa taatcgatat
    12301 ctttctgaat gcattaacca ctccaatagc acaagaactt ttaaacaaaa aaatggtgat
    12361 ggatatttta gccatgaaaa cacgtgatgg tgaaccaggg ttatttgctg caatggaaaa
    12421 taatcaccct ttgtgtgtca cacggttcct ctctaaagtt tatgggatcg ccgttaaata
    12481 taaactcagc aaaatcaaca tcatggactt attaaaaggc gcgacagcac agggaacccc
    12541 tgcattatac atcgccatga gcaagggtaa tgaagacgtc gtgttatctt atatatcgac
    12601 gctgggtgct tttgcaaaaa aaacattctt ttagtcaaca tcagttattt acactattgg
    12661 ccgctaaaaa tcatgacaac atgtcagctg ttcatatagc cattcatcat aagcattata
    12721 aaactgtaga aacatattat gctgctataa atgtcatcag ccaaagcctg agttttagtg
    12781 ctgatgaaat aaagacgtat ttataaccag ctatttacga tatatatcga tacataattt
    12841 tatttttttc ataaaacgca gaaacagtga ccaggtgtac aacgccaccc aacggctgta
    12901 ataacaaaaa atcccttgat gcccgtccct tttgttacac tccgttatca cgcacaagag
    12961 atatgcagga cactggtatg ccgactaaac gctttgataa aaaacactgg aagatggtgg
    13021 tggtgctact ggcaatctgt ggcgctatgt tgttgctacg ttgggcagca atgatttggg
    13081 gctgagcagt gacaaacgaa gacagccaga ccgcataaca cggtccggca gataatttta
    13141 catgctgtag cccagcgata aggttgtccg acggtcggta tgttccggtg cagactctgg
    13201 aggttcagag ttccatgtga cgttgtaagc caccttcagt ccaaaatgtt cattaatagc
    13261 aacatttaat gcgctttcgg agttcagcgt tgtgtcttcc gcgccaaaga ccgaaacacc
    13321 ctgcgtaaat ttagcgttgt cagtcaactg ccatgcataa gccccggagg catagcccag
    13381 cggctgggtt tcactggcat tgtcggtata tttgtcgtaa cgcacacctg gaccgaattc
    13441 aaagcggaaa ctgtgtaccg ggccattcaa aaactgatga ccataacccg cggtcaacac
    13501 atcgcgctct cgatagccgt tataacggtc tgtcagccag cttgcctgac caaataaata
    13561 gtcataatca gttaaattaa aacggctacg cccgcccgcc gcatattttt ctgaagaacg
    13621 ctcatcatta gaagaagtat tactggcgtt cccccacagc gaccaggcag tggtttgtcc
    13681 ataccaggtc atggtggtgt cagccgtaag tgaggagctt ttcgtattgc ctgattgtgc
    13741 aagatatcct gcgttcagat taccttcgaa aggtttttta gcgctggcag gatcgtccat
    13801 gacagtaaaa acggaatcat cggcagctgc attcagtgac gcaaacatgc cccccgccaa
    13861 cataacgatg gcaggaactg tcttcaaaag cttcatttat caagagtccg tacaacaaaa
    13921 aaagagacca tcgcggtccc ggaaactttc ttaaggatca aagattagcg tccctggaaa
    13981 ggtaacgaat tataaaaagg cg
//
LOCUS       DAZKGS010000119        13911 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKGS010000119 DAZKGS010000000
VERSION     DAZKGS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..692)
                     /locus_tag="ACHOQY_003473"
                     /pseudo
     CDS             complement(1..692)
                     /locus_tag="ACHOQY_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            693..1430
                     /gene="tagH"
                     /locus_tag="ACHOQY_003474"
                     /pseudo
     CDS             693..1430
                     /gene="tagH"
                     /locus_tag="ACHOQY_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
     gene            1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHOQY_003475"
     CDS             1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHOQY_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9679513.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            1954..3284
                     /gene="tssK"
                     /locus_tag="ACHOQY_003476"
                     /pseudo
     CDS             1954..3284
                     /gene="tssK"
                     /locus_tag="ACHOQY_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3289..4050
                     /gene="icmH"
                     /locus_tag="ACHOQY_003477"
     CDS             3289..4050
                     /gene="icmH"
                     /locus_tag="ACHOQY_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP9679514.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4059..6878
                     /gene="tssH"
                     /locus_tag="ACHOQY_003478"
                     /gene_synonym="clpV"
     CDS             4059..6878
                     /gene="tssH"
                     /locus_tag="ACHOQY_003478"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9679515.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESESEQDNTGAEPADEAGSEQPEET
                     AETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSV
                     ITDLTKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVL
                     QLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVV
                     LLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDP
                     ETMQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAE
                     VIIEPEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSA
                     VDGAFTADVEDAQNDESVTKDETVL"
     gene            6875..7618
                     /gene="vasI"
                     /locus_tag="ACHOQY_003479"
     CDS             6875..7618
                     /gene="vasI"
                     /locus_tag="ACHOQY_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP9679516.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            7623..9035
                     /gene="tssA"
                     /locus_tag="ACHOQY_003480"
                     /pseudo
     CDS             7623..9035
                     /gene="tssA"
                     /locus_tag="ACHOQY_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            9144..12578
                     /gene="tssM"
                     /locus_tag="ACHOQY_003481"
     CDS             9144..12578
                     /gene="tssM"
                     /locus_tag="ACHOQY_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP9679517.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            12589..>13911
                     /locus_tag="ACHOQY_003482"
     CDS             12589..>13911
                     /locus_tag="ACHOQY_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9679518.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLGPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVINQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPE"
BASE COUNT         3122 a         3955 c         4020 g         2814 t
ORIGIN      
        1 ccgtagtgga ccgacacgcg gtggccttca ctggtatggc tgagggtggt taaccacccg
       61 tgctcatcat actgccactg ctctgccggg tcgccgttca ccgtccggtg cgtgatgcgg
      121 tccgacgcat cgtagtgcca gagggtgata agcccctcgt cttcactctg tgtgagtttt
      181 ctggtcaggt catagtggta acgttgcgtc cggccgtcaa aaccgcgctg ttcagtcagc
      241 ctgtcaaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacggtg
      301 atgcgcccgg cagcgtcata ccccatgctg cgcgtcagac cgccttccgt cacactcacc
      361 ggacgcccgc gtttatcata tgcgatggtg ctgcgtttac cgtccggtga taccgttgct
      421 gtcaggtctc ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccttctga
      481 ctgaccagtt gcccacgcgg gttataactg ctgtaagtgc tgatgccttc ctcccggtga
      541 acggcgattt gttgaccgta ccggtcgtac tcataccgcg tcgtgtaccc cgagcagtcc
      601 gtaaaggcca gcagttgacc gtaacggctc catgccatct gttttgtact gcccgtcgcg
      661 tcctgtatcc ctgtcggcag ctcactcgcc ggccgctttc aggcattgcg gctgtccggc
      721 catcggcacc gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc
      781 tgccgcccat ttatgccagc cccggcgatc gtaatgacga cgtctctgcg gcagaaatgg
      841 cgcaacgcca ccttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga
      901 gcaattccga cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa
      961 taaaaggtct gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc
     1021 cgctggaaga taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt
     1081 ttgccgaagg taaaagcccg gtacatctgg cggctcccgc cgccgtcagt gaaagcctgc
     1141 gcaatgtccg ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc
     1201 tgctggatgc tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc
     1261 atgaactgcg ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg
     1321 aagaactggc ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc
     1381 aagtctatga ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc
     1441 gtttctggcc tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat
     1501 cagtgaggtg atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga
     1561 aataaccgtc accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc
     1621 cgccgtggat gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta
     1681 tgaccagata gccagtacgc cgctaccgga tgtgctgggt aaaaaatata tcgaccacca
     1741 ggatttcaac ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa
     1801 aacgcagttt attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca
     1861 aatcgagacg gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag
     1921 cagcatcgaa atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg
     1981 tggcaggaag ggatgctgat gcgtccacac catttccagc agcagcagcg ttacaacgac
     2041 tacctggata accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc
     2101 actctcaaca atgaactgct ggcacagggt aagatcatga ttgacagcgc gtcaggcaca
     2161 ctgcccgacg gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac
     2221 ccgcaacatt ttccggacga gagaagccgc aatatctacc tcgctctgcc ggtcgccagt
     2281 gatgtgagaa atgaaatcag cgacgggcgg cgaatcgggc gttaccggct gaattatgcc
     2341 gatgtccggg atttgcattc agaagaaggt gacacgcgaa cactgacgct gggacaactg
     2401 acgccgcgca tatgagcggt gcagaagata tgagcgccta tattacgctc ccgctttgcc
     2461 gtatcagcga tcgccatgcc gatggctccc tgacgttgga tgacgatttt atcccctcct
     2521 gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta cagggggcca
     2581 ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag agcggcatcg
     2641 cggatgtggc ggaatttatg atgttgcagt tacttaaccg taaccagacc cggtttaccc
     2701 atcgcgcccg ccgatcccag ctccacccgg aagatttcta ccttgatctt gctgggttgc
     2761 tgggtgaact gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt gatgtgtatg
     2821 atcatcatga tctgaccaaa acatttaaaa cactgttacc ggaagtcaaa cgggcgctgc
     2881 ataccgtact gtcgccaaga gcggtcaatc tgccgctgca tctgcgtgac ggtatctggc
     2941 aggccgatat ccatgacaca gaactgctgc aatctgccac ctttgtgctg gccgtggcgg
     3001 caaacgtgcc ggtcgatcag atccagcgtc agtttatcca gcagtcgaaa atttcctcgc
     3061 cggaaaaaat ccgcaatatg gtcagtgtgc agatccccgg cattccattg cgcgcactga
     3121 tggtggctcc ccgccagctt ccttaccatt ccgggttcag ctatttcgaa ctcgacaaga
     3181 gcggacaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat gtatccggca
     3241 gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat ggatgagggg
     3301 tcactgtctc tgccaccatt taccggatac gacgaaaaaa gccagcgcga ctatcatctc
     3361 gcgcttcgtg gcaacagcct gaatccgatg atcgacgcag ccacgccgct cctcggtatg
     3421 gtgatgcgcc tttcaaccat gaacagccag acgatgccgg agcatctttt tgctcaggtg
     3481 gtgacggatg ttcaggccgt ggagcaactg cttcaggagc agggctacga accgggcgtg
     3541 attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct cggtaacggc
     3601 tggtcgaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca caacgaagcc
     3661 tggggtggag agaaagtttt catcctgctg gagcgcctga tccgtgaacc caaacgttac
     3721 caggatctgc tggaatttct ctggctctgt ttttctctgg gatttcgcgg gcgctacaaa
     3781 gtggcagcac aggatcaggg ggaatttgag cagatctacc gccgcctgta ccacgtcctg
     3841 cataaactac gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa aacccagggc
     3901 gggcgttatc agctcatcag tcgcctgacc attaaacata ttttttgtgg tggcgttgtc
     3961 gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac gcaggacatc
     4021 ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt gatcttccca
     4081 cgctggtaaa acggcttaac ctgttctccc gccaggcgct ggagatggcc gcctctgaat
     4141 gtatgagcca gcaggcagcg gaaattaccg tcagccatgt gcttattcag atgctcgcca
     4201 tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg gaagagttgc
     4261 gccaggcgct gacggtggag aactacacaa ctgcccgttc tgcggacagc tacccggcat
     4321 tttccccgat gctggttgag tggctgaaag agggctggct gctggcgtcg gctgagatgc
     4381 agcacagcga attgcgtggc ggcgtgttgc tgctggccct gctgcattcg ccgctgcgtt
     4441 atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt ctgcaacagg
     4501 actttgtgca atggacacag gagtcggcgg aatcagtcgt gccggatgca gacggtaaag
     4561 gcgcaggcac actgacggac gctgctgaca ccctgcttgc ccgctacgcc aaaaacatga
     4621 ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac gaaatcgacc
     4681 tgatgatcga cattctctgc cgccgccgca agaacaaccc ggtggtggtg ggcgaagcag
     4741 gcgtgggtaa aagcgcctta atcgaagggc tggcgctgcg catcgtggca ggccatgtgc
     4801 cggacaagct gaaaaacacc gatatcatga cccttgactt gggcgcattg caggccggag
     4861 cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct gatggcagag gtcatttcct
     4921 ccccggtgcc ggtcattctg tttatcgacg aagcacatac cctgattggc gcgggcaacc
     4981 agcagggcgg gctggatatc tccaacctgc tcaaaccggc gctggcgcgc ggcgagctga
     5041 aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgaaaaa gatgccgccc
     5101 tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa cgctgccgaa gccaccatta
     5161 ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt gatgatgacg
     5221 ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc cgggcgtcag ttaccggaca
     5281 aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg tcgtcgccgc
     5341 cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa attcgccagc
     5401 ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc gaggtgctgg
     5461 tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcctgg caccagcagc
     5521 agacgctggt ccgggagatt attgcgctgc gccagcagtt actgggcgtg gcagaggacg
     5581 atgcggcgcc gttgccggac gcagataccg tggaggatac gcagccagag tcagagtcag
     5641 agtcagagtc agagtcagag tcagagtcag agtcagagtc agagtcagag tcagagcagg
     5701 ataataccgg tgccgaaccg gctgatgaag ctggcagcga acagccggaa gagaccgctg
     5761 aaacagtttc cccggtacag cggctggccc agctcactgc cgaactggac gccctgcata
     5821 acgaccggtt gctggtctcc ccgcacgtcg ataaaaaaca gattgcggcg gtgattgccg
     5881 aatggaccgg cgtgccgctc aaccgcctat cgcagaatga aatgtcggtc atcaccgacc
     5941 tgacgaaatg gctgggtgac accatcaaag gccaggacct ggcgattgcc agcctgcata
     6001 agcatctact gaccgcacgc gccgacctgc gtcgtccggg acgcccgctc ggtgcgttcc
     6061 tgctggctgg ccccagcggt gtgggtaaaa ccgaaaccgt cctgcaactg gcagagctgc
     6121 tctacggcgg tcgccagtac ctgaccacca tcaatatgtc cgaattccag gagaaacaca
     6181 ccgtctcgcg gctgattggt tcgcctccgg gctacgttgg ctacggtgaa ggcggcgtac
     6241 tgaccgaagc gattcgccag aaaccctact cggtagtact gctcgatgaa gtggaaaaag
     6301 cgcacccgga tgtgctcaac ctgttctacc aggcgttcga taagggcgaa atggcagacg
     6361 gtgaaggccg cctgattgac tgcaaaaata tcgtcttctt cctgacgtcc aacctcggct
     6421 accaggtaat agtcgagcat gccgatgacc cggaaaccat gcaggaagta ctgtatccgg
     6481 tgctggccga cttcttcaaa cctgccctgc tggcgcgtat ggaagtggtg ccgtacctgc
     6541 cgctgtcgaa agagacgctc gccaccatca ttgccgggaa actggcccgc ctggataacg
     6601 tgctgcgcag ccgctttggc gcggaggtga ttatagaacc ggaagtgacg gacgaaatca
     6661 tgagccgcgt cacccgcgcg gaaaacggcg cgaggatgct ggaatcggtc atcgacggca
     6721 atatgctgcc gccgctctcc ctgctgctgt tgcagaaaat ggcggcgaat acggcggttg
     6781 cccggattcg gttgtcggca gtggacggcg catttacggc agacgtggaa gatgctcaga
     6841 acgacgagtc cgtcacaaag gatgaaacgg ttttatgagc aggttgcgcg ttttccggca
     6901 gacaggatgg tttatcgcgg gcctgatgac gggcttaccc gccactgccg cgcccgcaga
     6961 agcatcgtca atggccggtg tggcggtcgc cgtcgccacc acgaccccgc cggatgcgac
     7021 agccacgtta caggccatgc agtcctgtcg gcgggagtcc gcggcgctgg agcggcttga
     7081 ctgctatgac cgcctgctgg caccgctgtc cccgtcaggg tttgacgggg cgctggtcaa
     7141 agccggtttt gtgggcgagg cctggacccg cgccacagag caggaaaaac gccgcgaggg
     7201 caacaccaca gaactgctgg tgacgcaggt gccgggggag cgcccgacgg tggtgataac
     7261 caccccggct atcggtcatg ttccgccacg cccggtgctg atgttcagtt gcgtggacaa
     7321 tatcacccgg atgcaggtgg cgctgatgca ccctctggat gtccatgata tcgccgtcac
     7381 tctgaatgcc gacaaccggg cattgcgcag ccactggttt gtgcgcgaga acggcaccct
     7441 gctggagtcc agccgggggt tatccgggat tgatgaaatt aagcaactgt tcggcgcaaa
     7501 aacgctgacg gtggataccg gcgcagacaa tgccgccgga aagctgactt ttaacattga
     7561 cgggctggca agggcgattg ctcccctgcg cgatgcctgc cactgggcgg gagaataaaa
     7621 atatggcaat ggacttacgc gatccgaatg tctggatatc gcacctgctg gaaaacctgc
     7681 cggaagagaa actggcatcg gcgctgaaag atgacaaccc gaactgggag tatatcgacg
     7741 gcgaaatcgt caagctggga tcgctggccc acagtcaact ggatatcccg gaactccagc
     7801 gccgtgggct ggtcattctg gcttcggaga gtaaggattt tcgtctgctg gcgcatctgc
     7861 tgagaaccct gcaacacgcc ggtgatcctc atctcgcatt gcgcctgctg gcgctgtatg
     7921 tggagcatta ctgggcggtg gctgcgccgc agaatatggc gcataaaaaa cgctttgcca
     7981 gccaggtcat taaacgcttt gaaacaggta ttgaagtttt ttcacagaac gccgccacag
     8041 cgcagcgcga tgcgctgtca ggggaactgg caaaactggc gcaatgctgg cagtcacata
     8101 acgccccgga actggcacag gctactgatg atctttttgc cctgtaccag cgtgcgttta
     8161 atcgtgcggc accggctcct gtccccactc cggcggcctc cggtagttca ccacagacca
     8221 ccgccacgtc tgaaagcggc gtgacgcaac ccagtgctcc ggctccccaa atcgtcatcg
     8281 acagtcacga cgacaaagcc tagcgcgaca cgctgttaaa agtggcggcc attttatgtg
     8341 aacgccagcc ggattcaccg cagggctacc gtctgcgccg ccatgcgctt tggcagaaca
     8401 tcaccagtac gccccaggcg gaaagcgatg gccgtactcc actggctgcg gtctccgccg
     8461 atatggtggc agattaccat gctcagctcg gtagtgccga tatggcgctg tggcagcagg
     8521 tggagaaaag cgtactgctg gctccttact ggctggatgg tcactgcctt tctgcacaga
     8581 cggcgctgcg tctgggttac aaacaggtgg cagacgccat ccgcgatgag gtcatccgct
     8641 tccttgagcg tctgccccag cttaccgggc tgctgtttaa tgaccacaca ccgtttatca
     8701 gtgagcagac gaaacaatgg ctggcagctt cgcccgacgc caaagttgca ccggttgcgc
     8761 aaatcggtga ggaatcgaag gcagccagag cctgttttgc tgagcagggt ctggaggcgg
     8821 cgctgcgata tctggacatg ctacccgaag gcgatccccg cgatcagttt caccgccagt
     8881 accttgccgc acagttgacg gaagaggcgg ggctggtaca gcttgcgcag cagcagtacc
     8941 ggatgttgtt caggatggga ttgcagatga tggtggcaga ctgggagccg tccctgcttg
     9001 agcagctaga acaaaaattc acggcagaac aataaaagag aacaggagtt tttgtgttca
     9061 gattaccaac accccgacta ctcagcggac tcaaatcagc cctgcgaccg gcgatgcccc
     9121 ggtttaaagt ctctgctttc tggctgctga tactggcgtg gatttttctg ctggtgtgga
     9181 tctggtggaa aggcccgatg tggacgctgt atgaagaaca gtggctcaaa ccactggcga
     9241 accgctggct ggcaacggcg gcgtggggga ttattgccct ggtgtggctt accgtccggg
     9301 tgatgaagcg cctgcaacag cttgaaaaaa tgcaaaagca acagcgcgag gaagccgttg
     9361 atccgctcag cgtggaactg aacgcccagc agcgttatct tgaccgctgg ctgctgcgcc
     9421 tgcaacgcca tctcgacaac cgccgtttcc tgtggcagtt gccgtggtat atggtcatcg
     9481 gcccggcggg cagtggcaaa acgacactgc tgcgcgaggg gtttccgtcc gacattattt
     9541 atgccccgga gggcgcacgc ggcgcagaac aacgcctgta cctcacgccc catgtcggta
     9601 aacaggcggt gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc
     9661 tgcatcgccg tctgtgggaa catgccctcg gctggctgaa ggaaaagcgc gcacgccagc
     9721 cgctgaacgg gattattctg acactcgatt tacccgatct gctgaccgca gacaaacgcc
     9781 gccgcgagca tctgttacag acgctgcgca gccgcttaca ggatatacgc cagcatcttc
     9841 actgccagtt accggtttac gtggtactga cccggcttga tttattacag ggttttgccg
     9901 ccctgttcca gtcactcaac agacaggatc gcgatgcgat tctgggcgtc acgttcaccc
     9961 gccgcgccca tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg
    10021 tggatcgaat gaatctggcg ttgccggatc tgatggtcgc tcagactcac acccgcgcgt
    10081 ctttattcag tttttcccgc cagatgcagg gaagccgtga accgctggtg tcactgcttg
    10141 agggtctgct tgatggcgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctctt
    10201 cgcttcagcg tgggcagatg gatgatatat tcacccagtc tgccgcccgc cagtaccggc
    10261 tgggcaataa cccactggcg tcctggcccc tggtggacac cgcgccttat ttcacccgca
    10321 gcctgttccc gcaggcatta ctcgcagagc ctaatctggc aacagagagc cgcgcctggc
    10381 tgatacgttc ccgtcgccgc ctgacggttt tctctgccac aggcggcgtg gcagcactgc
    10441 tgctcatcac cggctggcat cactattaca acggtaacta tcagtccggc atcaccgtgc
    10501 ttaagcaggc caaagccttt atggacgtgc cgcctccgca gggggaagat gactttggca
    10561 acctgcaact gccgctcctg aacccggtac gcgatgccac actggcctat ggcgactggg
    10621 gcgaccgcag ccgtctggcc gatatgggac tgtaccaggg acgacgtatc gggccttatg
    10681 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
    10741 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
    10801 taatgcgaat gctggaggac aaaagcggac gtaacaatga ggtggtgaag cagtatatgg
    10861 caaaacgctg gagcgaaaaa ttccacggcc agcgcgatat ccaggcacaa ctgatgtccc
    10921 atcttgacta cgcgctggct catactgact ggcatgcaga gcgtcaggcg ggcgacggtg
    10981 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
    11041 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
    11101 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
    11161 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
    11221 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
    11281 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
    11341 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
    11401 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
    11461 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacaa cacacagcca ggcgtctttt
    11521 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
    11581 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
    11641 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
    11701 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
    11761 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
    11821 cgctcaaccg ctgggttggc aggctggctg accaggcctg gcatgtggtg atggtggagg
    11881 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
    11941 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
    12001 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
    12061 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
    12121 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
    12181 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
    12241 tactgaacct tgacggtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
    12301 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
    12361 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttcg
    12421 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
    12481 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
    12541 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
    12601 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
    12661 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
    12721 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
    12781 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
    12841 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
    12901 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
    12961 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
    13021 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
    13081 tttatgcaga acacgcgtaa acggcttgga ccccagctga agagtaacac tgagaacgcc
    13141 gcacagacca cggtcagaac gctgatttat gccccggaaa cacaggcatc ttccacacca
    13201 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt tcgcagtctg
    13261 acagagaatc ccccacaggc cagtgtgata aatcaaggca gtacggtaag agggtttatc
    13321 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
    13381 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
    13441 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
    13501 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
    13561 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
    13621 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
    13681 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
    13741 caggcggagc ggggactggg gcaggaaaca cccgttgagt ctctgttgac gcagtaccat
    13801 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
    13861 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga g
//
LOCUS       DAZKGS010000120        13445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKGS010000120 DAZKGS010000000
VERSION     DAZKGS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(223..942)
                     /gene="dcuR"
                     /locus_tag="ACHOQY_003483"
     CDS             complement(223..942)
                     /gene="dcuR"
                     /locus_tag="ACHOQY_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9679519.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(939..2570)
                     /gene="dcuS"
                     /locus_tag="ACHOQY_003484"
     CDS             complement(939..2570)
                     /gene="dcuS"
                     /locus_tag="ACHOQY_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9679520.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2751..2981
                     /gene="yjdI"
                     /locus_tag="ACHOQY_003485"
     CDS             2751..2981
                     /gene="yjdI"
                     /locus_tag="ACHOQY_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9679521.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2993..3265
                     /gene="yjdJ"
                     /locus_tag="ACHOQY_003486"
     CDS             2993..3265
                     /gene="yjdJ"
                     /locus_tag="ACHOQY_003486"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9679522.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3492..3788
                     /gene="ghoS"
                     /locus_tag="ACHOQY_003487"
     CDS             3492..3788
                     /gene="ghoS"
                     /locus_tag="ACHOQY_003487"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9679523.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3816..3989
                     /gene="ghoT"
                     /locus_tag="ACHOQY_003488"
     CDS             3816..3989
                     /gene="ghoT"
                     /locus_tag="ACHOQY_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP9679524.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4108..5625)
                     /gene="lysS"
                     /locus_tag="ACHOQY_003489"
     CDS             complement(4108..5625)
                     /gene="lysS"
                     /locus_tag="ACHOQY_003489"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9679525.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5862..7319)
                     /gene="dtpC"
                     /locus_tag="ACHOQY_003490"
     CDS             complement(5862..7319)
                     /gene="dtpC"
                     /locus_tag="ACHOQY_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP9679526.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7378..9525)
                     /gene="cadA"
                     /locus_tag="ACHOQY_003491"
     CDS             complement(7378..9525)
                     /gene="cadA"
                     /locus_tag="ACHOQY_003491"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP9679527.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9605..10939)
                     /gene="cadB"
                     /locus_tag="ACHOQY_003492"
     CDS             complement(9605..10939)
                     /gene="cadB"
                     /locus_tag="ACHOQY_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP9679528.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11305..12843)
                     /gene="cadC"
                     /locus_tag="ACHOQY_003493"
     CDS             complement(11305..12843)
                     /gene="cadC"
                     /locus_tag="ACHOQY_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP9679529.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(13103..13217)
                     /locus_tag="ACHOQY_003494"
                     /pseudo
     CDS             complement(13103..13217)
                     /locus_tag="ACHOQY_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         3712 a         3077 c         3120 g         3536 t
ORIGIN      
        1 ggtaaaaata cacataatgc gggtgacata atagttaatt aacttttgtt atcgttttga
       61 aattaaaaac accgttcacc tgaagagata ttaattttta gcgatgatgg agggataatt
      121 atatttgatc tggcacaagt tttactgatg aaggatgtaa cttgtgccag gggtattttg
      181 cattacggta ataattatta cttacagata accagcgatc cgttattggc aatattgttt
      241 cagtagtgag tagtgttctg cctgaatacg gtaacggtaa accggacgcc ccgtgacgcc
      301 ataatggata ctggtgaaca agatgtggca gttgaccagc cagatgaggt atttacggca
      361 ggaaacacgc gagatgttaa cctcgttagc cagctcgtcg gttgaaaact catagtcctg
      421 atgcgcgtca atccactggc acagcgtgcg taaagtctgc ggcgttaagc cttttggcaa
      481 gcgacgagga tcctgttcgt tggagctgct gccgtggatt agctgatcaa gctcggcctg
      541 gtcataatac tgatgttttt ccagcgccat tttcttttgc cgccagccgg tgagcgcctc
      601 ttcaaagcgg gaagcctgga agggtttgat caggtaatcc acgacaccgt aatgcagcga
      661 atctttaatg gttgccgcat cggctgcgga ggagatgaca atcacatcac ttttgcaacg
      721 cgcgttatgc aggacaggca gtaaatcgag cccgttctct ttttgcatat agatatcgag
      781 caatatcagg tcgataggcg tatcgctatt gaagataatc tctttggctt tctccagcgt
      841 cgaggctgtt ccacagcatt gaaagcctgg gatttgtgct acgtatcggc gattcagctc
      901 cgcgaccatt gcgtcgtcat cgataattaa tacattgatc atctgttcga cctctccccg
      961 tcccagggta tctggacaaa aaattgtgtg aaaatcccgg gttccgattc cacggcgatg
     1021 ctgccgccga gattttctac ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt
     1081 ccttttgtcg agacaccttt gtcaaaaatg tgatcgattt tgtcgggtgc gattcccggt
     1141 ccatcatcgt taacttcgca gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt
     1201 tcgccaccgg gttccggccc taatgcttcc agcgcgtttt ctatcagatt tcccaatgtg
     1261 gtaatcagcg tcgcgacctg gtcctcactg ccgctgtctg gcagctggct ttcactgttt
     1321 aaaatcagcg tatggcctaa atcggtcgcg cggttaatct tgctgattaa aaaaccagcg
     1381 ataaccggag atttgatctt acccagcaga gagccaatct cttcctgata gttattggct
     1441 gttttgagaa tgtaatcttc caactgctta taactcttca gatgcaataa tccgagaatc
     1501 acatgcaatt tattcataaa ttcgtgggat cgttcacgaa gtgcatcagc atagttgacc
     1561 agaccgtcga gtcgctgcat cagtttacgt acttcagttt tgtccctgaa ggttgaaatg
     1621 gcaccgatga taacgccatt actgcgcacc ggaacggtgt tgatcagtaa tagccggtct
     1681 ttaatcgtaa tctcttcgtc gcggcgcggc gtaccgtcgc gtaacacttc cgagacatct
     1741 accacctgtg accatgagtg gctgagcgtc gacagtttct catcgtcctg cgacttacgg
     1801 taattcagca attcttgtgc ggcatcgttg atcagcgtga cctcgccgcg atcgtccacg
     1861 gcaacgacgc cttctttgat agactgcaac atggcctggc gctgctcaaa cagcgtggag
     1921 atttcgtagg gttccaggcc gaaaaggatt tttttcagta ccttaaccag aatgcaggtg
     1981 ccaatcagtc cgaccagcat gccaaataat accgaccaga taatgctcca gcgactgtca
     2041 ttgatttgtt gggtcacgcg gcttaactca aggccgatcg ccaccacgcc aatttgttta
     2101 tgattttcat cgtagatggg ggtaaatacg cgtaaagcct gcgccagaaa accgcgattg
     2161 atagcgacat tttctttgcc attcagcgct ttaaggatgt catcaccttt aaatggctga
     2221 ccaatacgct gggcttcagg atgcgagtag cgaagacttt gcatatcggt aacgacaata
     2281 aacagcagat cgttgcgttt gcgcacggct tccgcgatgg cctggattcc gctctcctgc
     2341 ggttttttct gcaagccctg acggatttcc ggcgagtcgg cgagggtacg cgccactgcc
     2401 agtgccttgt tggccagccc gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga
     2461 tgcaccacca atagcaccga gaacagtacc gcgctgacca ttaagatcac tgtggtactc
     2521 aatttcatcg gacgtttgcg taacatacgg tagggcaatg aatgtctcat cagcttcctt
     2581 gtgtgacaaa tttcttaagc attatctctg atgaggcggg taattcaaag ggagtaagaa
     2641 tgattggcta tataggggaa gagactctgg caacggaaac tgccagtgct gtatgaagat
     2701 tccagggcta tgcttatagc gataatcata ctgatgagag agggaaagtc atggatcagg
     2761 cgctactgga cgagggttat cgctgttata ccggcgaaaa gatcgatgtc tatttcaaca
     2821 ctgcgatatg tcagcattct ggcaattgcg tacgtggcaa cggcaagtta tttaatctca
     2881 aacgaaagcc gtggatcatg ccggatgaag tcgacgtcgt cactgtggtt aaagtgattg
     2941 atacgtgccc gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat
     3001 acgcgaaggc cacaataaat tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat
     3061 tgtctttgtg ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag
     3121 cctgaaaggg caggggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg
     3181 ggaaaaacga aaaattatcc cactatgccc atttgcgaaa cacgaatttg ataaaacgcg
     3241 ggagtatgat gatattcgca gttgatggga gagtacagag tcacgatatt tttcatgctc
     3301 tccgcggagt gatgcaggag agcatctgaa gggtaggggg atgcacaaag aatgggcaga
     3361 gagcgcgttt ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta
     3421 tatgagaatc atcaatcggg gttaataatt tttgcgtccc cagagcattc aatattgata
     3481 ggagtcatat tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc
     3541 catcatctta ttcctcagtg ttcttaaggt taagatcatt gatgtatgat atgaatttct
     3601 cttctatcgt ggctgatgaa tatgggatac cacgacaatt gaatgaaaat tccttcgcaa
     3661 taacgacatc gttagccgca agtgaaatcg aagatttaat caggcttaaa tgcttagact
     3721 taccggatat tgattttgac ctcaacatta tgacagttga tgactatttc cgtcagtttt
     3781 acaagtagcc aggaaggata agaagaacat aaaaaatggc actattctct aaaatattaa
     3841 ttttttatgt gattggtgtg aacatatcct ttgtcattat ctggtttatc tcacatgaga
     3901 aaacacatat tcgtttactt agtgcattcc tggtcggaat aacctggcca atgagtctgc
     3961 ctgtggcatt acttttttct ctcttttagg agctgtagtt gagtggaccc gctggttatc
     4021 gtgaaacgaa gcgtctctcc tcaaaactgg atttgcggaa cgacgaacga agcagtcgcc
     4081 tgactgcttc gttcattaaa gtgaaattta tttctgtggg cgcatcgccg ggaagagaat
     4141 aacgtcgcga atagtatgac tgtttgtaaa cagcataatc attcggtcga taccaatacc
     4201 cagaccagcg gttggcggca gaccatattc cagcgcagtc acgtaatctt cgtcatagaa
     4261 catggcttcg tcgtcacctg cagctttagc gttaacctgt tcctggaaac gttcagcctg
     4321 atcttctgcg tcgtttaatt cgctaaaacc attaccgatt tcacgaccac cgatgaagaa
     4381 ttcaaaacgg tcggtgattt ctgggttaac atcattacgg cgtgccagcg gtgacacttc
     4441 tgccggatat tccgtaataa aggttggctg aatcagatgt gcttctgcca cttcatcaaa
     4501 gatctccgtg acaatacgtc ccagccccca gcttttctct accgtaatac cgatagattc
     4561 agctaatgct ttagcagcat caaaattatc caggtcggcc atatcggttt ctggacgata
     4621 ttttttgatt gcttcgcgca tggtgagttt ttcaaacggt ttgccgaaat caaacacatg
     4681 ctcgccataa gtgactttag tggtacccag aacctcttgt gccagggtgc ggaacaggga
     4741 ctctgtcagt tcaatcaaat cgtggtaatc cgcatacgcc atgtagagtt ccatcattgt
     4801 gaactcagga ttatggcgaa cagaaatacc ttcattacgg aagttacggt tgatttcgaa
     4861 tacccgttca aaaccgccta caaccagacg tttcagatac agctccggcg cgatacgcag
     4921 gtacatatct aaatccagag cgttatgatg ggtaataaac gggcgagcag atgccccacc
     4981 tggaattacc tgcatcatcg gggtttctac ttccataaag ccgcgcgcga ccatgaactg
     5041 gcggatagcg gccagaattt ttgaacggac aacaaacgtt tgacgggatt tatcgttagc
     5101 gatgaggtcc agataacgtt gacgataacg gacttcctga tcctgcagac catggaattt
     5161 atctggtaaa ggacgtagtg cttttgtcag caggcgcaac tcagtacagt gaatggaaag
     5221 ctcaccggtt tgcgtcttaa acagcgtacc gcgggcaccg ataatgtcac ccagatccca
     5281 ttttttaaac tgatcgttat aaacaccttc tggcaggcta tctcttgcaa cgtacagttg
     5341 aatacggcca ccgacatcct gcaacgttac aaaggaggct ttccccatga tacgacgggt
     5401 catcattcgg ccagcaaccg agacttcaat gtttaaggat tccagttcct ggttatcctt
     5461 cgcatcaaac tcttcgtgca gctggtcaga ggtatggtcg cggcgaaaat cattgggaaa
     5521 cgccacacct tgctgacgta gtgccgccag tttttcgcgg cgatttctca gttcatcgtt
     5581 aaaatcaata gcctcattgg ctccccgtgt ttcttgttca gacattttgg ttcctctaaa
     5641 tccagctttc aatttagcgt agataaagag acagatcggt acactgtatt atctgcctcg
     5701 actattaata actcagttta tcttatcgaa actaacgctt acctcaagtt gatatcacta
     5761 ataaataacc atcaaaatgg tttaaatgac ttattaatct agatttgcta gtgttataaa
     5821 ctgaaagtta atttattcat aaccaattgt ttttacgaca gttaatcgtt gctctcctgt
     5881 atcatattcg tcggcgtgct aaacagaaaa cgggtagcaa aggccaatac gacgatgatc
     5941 gcgacacaag ccaacgtcca ctctcccatc tgagaaaaga atcgctggta agctgcaatt
     6001 gccataccgc taatttgcga ctctgtcgtc tgctgtgcca cgacgcctgc cagccagttg
     6061 gcgaccgcgc ctgttgccag catataaata ccggttaata cgccagacat tttcagacgc
     6121 gtgatttgcg caatcgccac cgggtcaata aagagttcgg caaagcccat tagcgccagc
     6181 ccggatatca tcacggccat tgacgcttga ccgccagccg ctgcatgtcg ggcatcaaat
     6241 gccaacaaca taaagccaca agccatcagc agtaagccaa aggcaaactt cagccagacg
     6301 cgcaatgttg agttgccgcg gctttctggc gacgccagcc aggccagtac aaccccagcg
     6361 agcatcactg caattgcatt caccgactgg aatagtgctg taggtacttc aatattgaat
     6421 gcctgacgat tcacaaagcg atcgataaac aagctgatgg tactgccgcc ctgttgtgcc
     6481 agtacccaga acaacgtccc gacaaacatc aacaatacaa tttgccaaag agcacggcgg
     6541 tgttcgggga atttgatcat catgcgggca atgatttgtg cggcaatgag gcaaacgatc
     6601 gccagcaaat atccggacca gtcgttctcc agcagcaggg taaaaaatac tggggctaaa
     6661 cagagcatca ccactaacca gctccatact ggtaaggcaa atttcacgct agtgagcgct
     6721 tttttatcca tactacgcgt ggactggaaa tgacgatgcc cgcttaagaa aatcaacaaa
     6781 ccgataaaca tgccgccacc cgcaagggca aagccaacat gccatccata ccactgagca
     6841 gccaggccgc aggcgattgg ggcggcgata gaaccgatat tgcccgcagc atacagcagc
     6901 gaaaaaccgc catcacgtcg atgatcgttc tcgtcgtaga gctcgccaag caaacagctg
     6961 atgtttgatt tgaataaacc gtagccacaa ataatgattg ccagcgccag atacaggcta
     7021 aaggttgaat ttgtatcaat gcccagcacc acatggccaa gggtcattaa cagcgcgccg
     7081 gcaatcactg cagtgcggtt gccgagcagg cggtcggcaa gccagccgcc gagaataggg
     7141 gtaacgtaaa ccagagaagc atatgcgctg aacaggctga tggcatggtt atcatcaaaa
     7201 ccaagctgat gggtgagata gagaatgagt aaggcacgca tgccgtaaaa actgaagtac
     7261 tcccagattt ggatcgccac gatatagtat atcgcgcgcg gctgtgaggg tgttttcatg
     7321 tgttctcctt atgagcaaaa aagggaagtg gcaagccact tcccttgtac gagctaatta
     7381 ttttttgctt tcttctttca ataccttaac ggtatagcgg ccatcagcct gacggtatgc
     7441 accgtgaata tcggtttcaa agcccggata gtgagcgccg atttcacaca gcatctgcag
     7501 gaactccaga accggacggc tttcttcggt gatcatttca cccggcatta ccagaggaac
     7561 tcccggcgga tacggaagga tcatattggc gttaatacga cctaccattt cgtcgaggta
     7621 aacttcttcg gtcataccgt gcagctcttt ctggaatgca gcatacggag tcattaccat
     7681 cgtcggcagc acttcaaatg cgcggtacat cagatccggc agattgtggt gaacaatcag
     7741 tttgtggata ttctgagcca gttcctgaat acgcatgttt tcatagaatt caggatcttc
     7801 acgatacaga gacggcagca tgtttttcac acgcaggttc aggtcgaacg cacgtttgaa
     7861 gtcagtcaga gcacgcagca ggctcagtgc tttggtctta tcgataccga tgctgaacag
     7921 gaacagcagg ttatacggac cggttttctc aacaacgatg ccatgttcgt cgaggtattt
     7981 cgccacgatg ctggccggaa taccaaagtc gctcatggtg ccgtcttttt ccatccccgg
     8041 agtcagcagg gtgactttga tcgggtcaag atacatgtgc tcgttatcga tgtttttgaa
     8101 gccgtgccag gtgctgtcag aacgcagcgg ccagcattca gtcgtatcga tatgatccgg
     8161 ctgccataca tcaaagaacc agccatcaga ttccgttctc agacgtttga tctctttacg
     8221 gaatttgatc gcacgttcaa tggaaccgtt gatcagacgt ttacctgcat tgcctttcat
     8281 catcgccgca gcggtttcag tggacgccac gataccgtag tgcggagaag tggtggtgtg
     8341 catcatgtag gcttcgttaa aggtttcttc gtttacgtca cctttaacgt ggatcatgga
     8401 agcctgagag aacgccgcca gcagtttgtg agtggactgg gtttcgtaaa tcactttccc
     8461 ttctacacgg ccaccgctca taccgcattt accttcgtaa atcggtgaga agttggtgta
     8521 aggcacccac gcggagtcaa agtggatgga tttcacatcc agtgttttct tgatgaagtc
     8581 ggtgttgtac agcagaccat cataggtaga gttggtaatt acagcatgta ccggccaggt
     8641 tgcgtttggt gtttctttca cgcgcttagc aatggtagcg tgctggaatt cactctgtgg
     8701 gataccacca agaataccgt aagcgttacg ggtcgggcgg aaatagattg gcgtaacatc
     8761 gctcatcatc atcaggtggg tcagcgattt gtggcagtta cggtcaatca gaatggtgct
     8821 gcctgccgga gcagagtaca tgccaacaat tttgttcgca gtggaagtac cgttggtcac
     8881 catgtagctg cggtctgcgt taaagacgcg agcgatatac tgttctgctt ctttgtgtgg
     8941 accactgtga tccagcagag aacccagttc agatactgaa atggaaatat cagatttcat
     9001 ggtattcgga ccaaagaaat catagaacag gctacctacc gggcttttct ggaatgcagt
     9061 accgcccatg tgaccaggag tacagaaagt atatttacct tcacgaacat atttaaacag
     9121 tgctttagtc agcggaggca gaatagtgtt gatatattcg tcagtggtct gcttgatctt
     9181 attagcaata tcttcagcag cacccagcgc atattcaaag aagctaatct gtaaacgcag
     9241 gtcattcagg cttacatcga gagtggaata cgtattagcg aacgcgtaca acggcaggtt
     9301 ctcgttcatt ttgctaattt cttcgcacag ctcgagatta tatttatccc agtcaaaaat
     9361 aacgccgcac agacgcgcat tgttttcgat cagttttaat aagtcgtcac ggtcgttcgg
     9421 gtaaacaatc tggaagttca gacgttcaag cgcgcgatga agttcacgga tgggttcttc
     9481 tttaaaataa acccccatgt gattcaatat tgcaataacg ttcatagtca tatctccagg
     9541 taaaaaaggc ccctcccaac acatgggaca aaatgaaagg aggagcctcg gaaaatactt
     9601 ttaattaatg tgcgttagac gcggtgtggt tatccattga gtggctctgg cgctcgtgca
     9661 ttttgcgagc gtagaacatc aggataatca ggctgacgat gaaggtacct gccagctcga
     9721 aggagcttgc gcccatcagc gcgatgaagc agaacacaca acccagtaca gagcagatca
     9781 ggctgacaaa gttgcggatg ttaacgcctt caaaacgaat caggtcaacg caagagtaga
     9841 agtacggcag catagtcagc agtactgcga taccggtcag ttcaccgaac aggtcagatg
     9901 ctttaccacc ggcagagttc atcagagtga tgaggatcat cagggcagtc attttcactg
     9961 cagccagcag cagacctttt ttcggaatac cgttgctgtc gacttcacca taaactttcg
    10021 ggaagttacc gtcgttagcg gcacgtacac ctgcctggcc taccaacatc atccaggagc
    10081 ccagagaagt caggcacgca aaggcggtga atgcagaaac cagcggcgca gcccagttac
    10141 cgaggatggt tgaagcactg attgcaaacg gagcaccgga agctgccatt acagaagatg
    10201 gatacatacc ggaaagcacc tgagtcgcag cgatgtaaac aatacctgct aaaccagtac
    10261 ccagcatggt tgccagcgga acggtacgtt tcgggttttt aaccatacca gtacttactg
    10321 ctgcggattc aacacccacg aaggcccaca ggcagagcag aatactttta atgatcgcat
    10381 gaccatcagt ggtatccgca gtattccagt tagctgcata agttgccgca tcaaaccaat
    10441 gccagccaac aatagcggtc attactaccg gaataagaac cagcaccaga ccaatagtgg
    10501 ttaaacggct tacccaagta ccgccgagca tatttacaaa ggtaaatacc cagacgatag
    10561 caatacaggc gatacccgcc ggaacaggat catttaatac tgggaagaag gtggaaagat
    10621 aagataccgc ggtaatacca atcgccaggt taccaatcca gttagcatgg taataaagaa
    10681 cacctgtctg aaaaccaaat gcaggggaaa tttctccggc ataagcaatt gggccacctt
    10741 gttgcgggtt ttttgttgcc agtcgggcat atacatacgc cagcgacatt gcaccaataa
    10801 tagagataat ccaaccccag atagcaatac caccgatact tgctaggttc gcaggtaata
    10861 atgcaatacc gctccccatc atattaccgg caacaacacc ggtacaggca aatagcccga
    10921 tcttcttggc agaactcatg ctcttctcct aatttcattt ttgaatttgg agtccgggtc
    10981 atgatgtata actatttcct gaccagacca aactggcgct aagaatactc acgaaaaaag
    11041 gattaatcct aaagattagg tgaaataaca caaaagtttc tgtaagtgag aacttgaggt
    11101 tttttattaa cacatcagga tcgcaagttg atatcatgaa aagataaaca ttcaatgttt
    11161 acaatggatt gcgggatatt ctctggttaa atttatgtaa taaaaattat gcggcaaata
    11221 aattgccgca acatattata ccaacagaaa catacaaaaa ctcaacaaca aatattcccg
    11281 agcataaatc aacccgggag ttacttattc tgaagcgaga aatttgtcga gataaggtac
    11341 aacataagga acagaagtct ggaatatacc attttcaatc cagtaaaggg tgtttgcccc
    11401 tgggcgtaaa ttaaaggcgg tgagatatgc atcagccgct tcccggttca tccccttcat
    11461 ttcataaacc ttgccaagca acacataatt tagccaggac atttcaagat caatgccagt
    11521 atttatcgcc tggtaagact catctgtttt accttttacc agagcactga ccgcttttat
    11581 ttgatatata atggacaggt tattcaattc cggcagtgta acaatgttgt ctatttctgt
    11641 gttcagtgct gctaattgtt tttcatctaa aggatgttga gaatggcgca cgatatcaac
    11701 taatgctttt tctgctctcg cgtaggtaaa ttctggggat gattgaacaa tctcacctaa
    11761 taattcactg gcacgattca atgatttatc atcgccatgc agtaaataat catgtgcctg
    11821 ataaaaatta gttaataacg caccacgatg aggcaatatt tgctggagcg tctcctgcat
    11881 tcgttgtggc cacggttggt ttaacgcttt tgataaactc tccagtaaat cattttgaat
    11941 cgccagctga ttaccgttag tgatgacata acgtttatcc agcatggttg aaccatctgc
    12001 attgtctacc aattttatcg acataaagca ttgttgagca cggtattggc gctgattaac
    12061 aaacgcaata gataatgttt taccggaact gctcggttca tcaatgttgt agttgatttt
    12121 gtcatgcacc ataaaggtgg agaaggtgtt aagtgatgtc gccaccaaat cacccacgcc
    12181 tatcgcgtaa gagagctgat acggggaact ccagctgtta caacttttat ttaccatatt
    12241 aatgtcaata tcgcgtggat tgagcaaaat acgcgatttg ctcataggaa gacgtgtatc
    12301 aagagttgaa aacgctacca gtgctacaca gatacctaac gacaacagga aaaaaaacca
    12361 tacccaaaag gtagtgaatc gtttgctttt aactggggat tgttcaggtg gcgttgcggt
    12421 gttttgaatg ttaagactgt gggagggaga atctgtggca ggaaccgcct ctggtatagg
    12481 gggaggcgaa gatagcatta tttcctctcc ctcttcttcg ctgtaccaga taaccggcac
    12541 cattaattta tagccgcgct ttggtacagt agcgatatag acaggactat cttcatcatt
    12601 atcttttaat gacttacgta gttctgagat actctgcgtc acaacatgat tggtgacaat
    12661 acttctcttc cagacattat cgataagttc atccctgcta agtacttcgc cactgtgttg
    12721 agcaaagaaa accagaagat cgattaatct cggctcaagg gtaagttgac gcccattgcg
    12781 gctaatttgg tttatggacg gagtaacaag ccattcgcca acgcgaacta caggttgttg
    12841 cataatagaa actcattcga aaagggaatg atgcaatgat aattgccaca acctattttt
    12901 accatctata gatgggttta tttacatatt attggtgaat gcaagacgtt atttttacca
    12961 gccataaact tcttgattac atagtattac gaaaggattt tactgagaac cagaagtaat
    13021 attccttacc atcaaaattc atcatctttg ccaaagaaaa atgttcagaa aataatccat
    13081 ggaaaattgt ccggagcgct tactatttta atggattgtt agtctttgca tgagcaagcg
    13141 gactgataca tttctctttg ttctcattca gaaaatctca tcagtcggcg ttcagaccgg
    13201 tgttgtgctt tatggatgcg ttacgctcct gatgacgtca tttgacgttc aacaacatca
    13261 cggggccgca cgacatttca cgtcagttag tgctatcgct caggaacaaa ttttcccgaa
    13321 ttgggatatg cccgcaaatt gctggtgatg tggggagaat ctggttgagt tcgggagaat
    13381 tgatttggag gcagaacgct taaattgtgg cgtccagaaa cgaaaaacgg acctccgtgg
    13441 aggtc
//
LOCUS       DAZKGS010000121        13386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKGS010000121 DAZKGS010000000
VERSION     DAZKGS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..1173
                     /locus_tag="ACHOQY_003495"
     CDS             115..1173
                     /locus_tag="ACHOQY_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP9679530.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            1183..2325
                     /locus_tag="ACHOQY_003496"
     CDS             1183..2325
                     /locus_tag="ACHOQY_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP9679531.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            2318..3091
                     /locus_tag="ACHOQY_003497"
     CDS             2318..3091
                     /locus_tag="ACHOQY_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679532.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            3093..4172
                     /locus_tag="ACHOQY_003498"
     CDS             3093..4172
                     /locus_tag="ACHOQY_003498"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP9679533.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            4172..5128
                     /locus_tag="ACHOQY_003499"
     CDS             4172..5128
                     /locus_tag="ACHOQY_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP9679534.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            5139..6347
                     /locus_tag="ACHOQY_003500"
     CDS             5139..6347
                     /locus_tag="ACHOQY_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP9679535.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            6365..6832
                     /gene="terW"
                     /locus_tag="ACHOQY_003501"
     CDS             6365..6832
                     /gene="terW"
                     /locus_tag="ACHOQY_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP9679536.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            7093..7422
                     /locus_tag="ACHOQY_003502"
     CDS             7093..7422
                     /locus_tag="ACHOQY_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9679537.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            7409..7789
                     /gene="tnpB"
                     /locus_tag="ACHOQY_003503"
     CDS             7409..7789
                     /gene="tnpB"
                     /locus_tag="ACHOQY_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9679538.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVEWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            7832..8662
                     /locus_tag="ACHOQY_003504"
                     /pseudo
     CDS             7832..8662
                     /locus_tag="ACHOQY_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(8693..10306)
                     /locus_tag="ACHOQY_003505"
     CDS             complement(8693..10306)
                     /locus_tag="ACHOQY_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP9679539.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(10337..10687)
                     /gene="tnpB"
                     /locus_tag="ACHOQY_003506"
     CDS             complement(10337..10687)
                     /gene="tnpB"
                     /locus_tag="ACHOQY_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9679540.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(10684..11109)
                     /locus_tag="ACHOQY_003507"
     CDS             complement(10684..11109)
                     /locus_tag="ACHOQY_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9679541.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            11163..11914
                     /locus_tag="ACHOQY_003508"
                     /pseudo
     CDS             11163..11914
                     /locus_tag="ACHOQY_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            13231..>13386
                     /locus_tag="ACHOQY_003509"
     CDS             13231..>13386
                     /locus_tag="ACHOQY_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679542.1"
                     /translation="MYLHTYQHNDRLPPQKHDKGIIMEFSVIFFWVITGLITILFLKS
                     IITDLIKN"
BASE COUNT         3233 a         3508 c         3480 g         3165 t
ORIGIN      
        1 gcactccata accacaggag ttattaatca tattatgatt gacatgtagc ttagacactc
       61 atagactcac ttctcaatca taaatttgca tggcgttccg gataattaaa aaaaatgaac
      121 aagaaaattt ggtttatgga aggtttatcc tcccagcgag atattattca gggggtaaaa
      181 tcatttgcac aaaaaaataa ttttgccatt accgtttttg cctcccaccg taacgaaaga
      241 aatgaaatcc tttccgttgc cgattattct ttgactgaac ctgaagatcc tcaaaaacgt
      301 cttcagttta tccaggaaac cattcagacc tacggcatcc accatattca tactggccgt
      361 aacagccagt ggtttgaaga acaccgttca gccattgaac cgaccggtgc cacccttact
      421 accggtgcaa cgggcgtcga ctggttaact ctggctgacg aaaaagttac ttttgctcag
      481 tttatggagc aaaagggtct cccggtcgta ccatcctggc gggtgaatac gctggcagaa
      541 ttaaagacac acctcgcggc cccgccgttc actgacagcc cggtatgcgt gaagccggtg
      601 acgggtatct atggcatggg attctggcgc tttgatgaca gtgcttcgcc tatggccgtc
      661 tttaatcatc ccgaacatcg tctggtcagt ccgcaacagt atattgcagc agcatcagct
      721 gctgagtcgt ttaaacccct tgttttgatg ccgtacctgc caggcccgga attttccgtc
      781 gatatcctcg cggataaggg cgaaatactc gcagccgtgg gacgccgtaa ggaaggggct
      841 atccagtatc tggtaaacga aggaagcgcc tgggaactgg cgtgtgactg cgcccgtgtt
      901 atgaaggccg acgggctggt gaatgttcag acgcgaaacg atgtgaatgg caacccggtg
      961 ctgcttgaaa ccaacatgcg tccgtcaggg ggggtgggtt atacccttca cagcggtgtg
     1021 aaccttcctg ggttatttgc tgcctttaag ctcggtctga tgtctgaaga tatggtacgc
     1081 cagagcgcta aaaacacctt ttctccggtt gcggtgagat ccattacgga tgtaattgca
     1141 tacccggaat cactctctaa ccttctgaat taaggcgtat ttatgtctga tatcctcgaa
     1201 gatatgattt atcgccgtac cctctcatgc ggtacgattc aggtaacccg tgaccagggt
     1261 gaagtttcgc tcgatgacct gtttgacatc gctgaaagac gtaacccgaa acgcgccttt
     1321 ctgtttgtca gcaaagtact cggcaggcac attcccgtgc caccctcagt catgcgacag
     1381 gcctacagac agcttgccag ccagttcccc tcgacactga caggacccgt actgtttatc
     1441 ggtatggctg aaaccgccgt tgggcttggg gccggtgtat ttgatgaagt gcgccaccag
     1501 catcgtgaat ctgtctatct gacctctacc cgtcacccgg ttgatggcac gttgctttgc
     1561 gagttcaagg aagaacacag ccacgccacc gatcatctga tctatctgcc agatgatgaa
     1621 gagaaaagac gtcgtgttac caacgcacga acgctggttt tgattgatga cgaggcaacc
     1681 accggtaata cctttattaa cctgctttct gccctgcgta ataccggcaa gcttcaacat
     1741 attgaacagg ttatcgccgt aacgctgacc gactggagtg gcaatgccct gtcggagcgc
     1801 acccccttgc ccgtcacttc ggtttcgctt gtaagcggtc agtggggatg gaccccatta
     1861 cctgatgcac ctgtcccgga catgccgaaa gtcaacgtaa cttcacgagg agaatgggac
     1921 atccagggga aacagtcatg gggccgactg gggatgctcg cacctgcggc cgatctcggc
     1981 catgaggtct ccgtccacaa gggggaacgt attctggttc tcgggaccgg ggaattcgtc
     2041 tgggagccgt tcctgcttgc tgaacggctc gaagctgccg gagcacaggc attttatgga
     2101 tcgaccaccc gctcccctgt cgccgttggt tatgccattg agtccgccat ttcctttacg
     2161 gataactacg ggctgggcat ccccaatttt gtctataacg tcgcccacca gcagtttgac
     2221 cgcattcttg tgtgtactga gacacccgca gaaagtattg acacgcagct tcttaaagcg
     2281 ctggctgagg ttgcgcccgt cgtggagatt gttacctatg aataaacccg ttgttctgag
     2341 cgaccttgac gacacgctct ttcagacccg tcgtaaaatg gtggacgagc tggccctgga
     2401 gccattccgt accggtgccg ttgatcgcac cctgaatcca cgaagcttta tgacggaaga
     2461 acagtccatg ttggtggact ggctcctgga gcaggctgaa ctcatacccg ttaccgcacg
     2521 gggaactgaa gaaatcagcc gcgtacggat ccctttccac tcctgggcaa tcacaacgca
     2581 cggggccgtc attctcacgc ctgaaggcaa acccgacgag gagtggaagg cccatatgct
     2641 cggccagctg gctccctacc aggaaaagct gacatcgatg cagcgtctga tcactgaaat
     2701 gatggacgca aaaggaatca atgcctgggc aaggctgaac ttcgaatacg gtgaaacggc
     2761 ggtttatatg gtgatgaaac accgcgacag cacccgcctt gacgagctca atgccattgc
     2821 agatgagata gaaacggtgt ttccgaccga gggcttctac atccaccgca acagtaataa
     2881 cgtggcctgg cttcccaccc cggttgagaa agggctggca gtcagctggc tgcttgaaaa
     2941 acttcgggct gaacgaggag ttttccccgt aattggtttg ggcgacagcc tgagcgatca
     3001 tcgttttatg aaactgtgca gctggtttgg catcccgcgt cagagtcagt ttgcagatgc
     3061 catttcacag cgaatttttg gagaaaatta aaatgcagcc acatgaaaca tttaccggct
     3121 cataccagcc cggtgacgtg gaatttctgc taaagccggt tgtcattgag atgacgccgg
     3181 ttgagaaaaa agaagaactg attcagtccg ggaagaaaca ttattcggac atgctcagcc
     3241 aggagccagc gcctacacaa tggcatcttg atttgtttca ccgggcgctg gatcggggcg
     3301 cagagaggct tgcaaaagaa gtcacacagc tcgctattgc tctggccgaa cgcttcggtg
     3361 atgagcccat tgtactggcc agtcttgtca gggctggcgt tccgctcggc gttatgctgc
     3421 accaggccct gcgtgacatg gggaaaacct catggcatta cggtatcagc attatccggg
     3481 atcgtggaat tgacggtgca gccctcgacg tcattgaaga gcggcatggt accagcggta
     3541 ttgtctttgt tgacggatgg acaggtaaag gcgcaattac cggagagctt gtacgcgcac
     3601 tgaaagatcg cccgggctat cctgagcagc cgcgactggt tgtcctggcc gatccctgtg
     3661 gctgctcctg gcttgcagcc agtgatgatg actggctcat tccttttggc atcatggggg
     3721 cgccggtatc aggcctgatc tcccggtcag tatggtcctc tgaaggatta cacgggtgca
     3781 tggtttgcga gcatctcagt gaattcgaat gcagccggat gcttgccgat accgtcgctc
     3841 atttccgtaa gaagttaaca ccgtcatccc tcgctcccct gagctggaat acggaatcag
     3901 cccgggtctt atggcagaca agtcgcgacg ttatcgcgtt cctggccgat gaatttaaag
     3961 tggacagcgt caatcgtatt aaacccggta ttgctgaagc aacccgggct gtattacgtc
     4021 gggtaccgga ccatgtattt gtgcgttcta ttgacgaccc ggacgttgcc ttgcttgtag
     4081 ggcttgctcg tgaaaaggga atagttgtta cagaaatggg gggaaccctc ggccagtatc
     4141 gggctgtaac cattatcaag aaggtactct gatgaaaaat cgtctttctc cctggaatct
     4201 gggagccacg ctatacatgc ctgcaacacg ggaagatatt gctgatgccg tcctgcacgg
     4261 gaaaatcccg ggcttgcgct ctctggtgat ttgcctggag gatgctgtca gtgaagcaga
     4321 catccccgtc gccctcaaaa atctggagca cctgctgcac gagctcagta acagcatgag
     4381 tagcctgggt aaaaatgact ggcctctggt gttcattcgc ccccggcatg ccgaaatggg
     4441 cagatggtta aaagcgcatt atgatctttc cgctgtcgac gggtttgtgc tgcctaaatt
     4501 taccctcagt tcgttagctg aatggtggga tatcatggtc gggactcacc tgtgcatgat
     4561 gccaacgctg gaaacagaag atgtctttga cgtggttcag atgcgcgagc tggctacccg
     4621 tctggaggaa catccctgcc acgaccgcat tattgcgctt cgaatcggcg gcaacgatct
     4681 tatgaacgtt gtgtcgcttc gccgtccccg ggacctgacg ctgtatgaca gcccgatggg
     4741 ctacgtcatc aaaatgctcg tttcggtctt cggcccgcgt gattttgccc tgaccgcgcc
     4801 tgtatgtgag catattgacg atcatgccgt tatggccaga gagctggctc ttgatatggc
     4861 acacgggctt gttgggaaaa cggccattca cccgggtcag atagaggtca ttcaaaacgc
     4921 gctgatggtc actcagggtg agcattctga cgctctgcgg atcctgaact ctacccaggc
     4981 cgtgtttaag tcgcagggag caatgtgtga acccgccaca catcgccgct gggcggctgg
     5041 cattctggac agggctcgtt tttatgggtt acagaacgag caaagcgctg atggaatcag
     5101 attacttacc gtgacccagc atcattaagg aaatcataat ggggttcagg ttcagaaagt
     5161 cgatcaacat tattcctggc gttcgcctca acctgagtaa cggtgcaccg agcctgagtg
     5221 tcgggccgag aggtgcttcc gtttcttttg gtagccgggg gacctatgcc aatctgggct
     5281 tgcccggtac cgggctgagt taccgtaccc ggcttgaccg ggccgcgcgt tccagaggtg
     5341 aaaaccggac ggcaaccgac ccggggctca gacaggcgct tgagcagaaa gccgctgaac
     5401 tcatgtcagc ggtaaccgca atccgtaata ttcacgagct gacgccggat ccaaaaacag
     5461 gcatcagctg ggcagagctg gaagcagtat acctgcataa cagaacgtcg ccttttcagg
     5521 ttccggcacc ggtgcgtcca gaaaagccag actaccttgt attgccggaa aagcctgccg
     5581 agagcgaagg cattagtttt ttgggtaaat ggtttgaatc ggaatcagct aaagctgagc
     5641 gccacgccga aaatcttcgc cggtggcagc aggagctgat tgatgtggag cgtgagaata
     5701 cccttcgaca gcaccggtac cagcaacaac ggacggcctg ggccgaacag tatgcaaact
     5761 ggaagtttga agctgaagaa catgaaaaac ggctcgccac ggctcaggca gatgcccggc
     5821 agcagttccg gacagacgcc gcgtttttcg aatcatacct ggcgggtgtg ctggcagaaa
     5881 ctgaatggcc gcgtgaaacg cttgttgcat ttgaagtaaa gccggagcta tcagcagtcc
     5941 tgctggacgt tgatttagct gagattgaag atttccctga taagatttac ggcgttaatg
     6001 cccggggaac ggagctgacg gaaaaagcca tgacgcaaaa agccgtacgc gaaaactatg
     6061 cccaccacgt ccatggctgc ttgttccgcc tggtcggtat cgttttacat acgctacctt
     6121 tcgacaacgt gattgtgtca ggctttacgc aacgggtcag taagcggacc ggctatctgg
     6181 aggatgagta tatcctgtcc tgcaaatgca ctcgcagcca gatgtcgtca gtaaattttg
     6241 caggcataaa acacattgat ccggttgaag cgttaggcga tcaaccggtt attcgaaaga
     6301 tgagcagtac cttcattttt cagcctattg aaccactaac cctttaagca ggctaaatct
     6361 tacgatgcaa ttaaatacca gacaggcccg gatttttaag cttgccaact tactgggcac
     6421 cggcaagccc gtttccgctg ccgacattat aaccagtctg gaatgctctg agccgacatt
     6481 aacccgggcc ctcaaagagc tacgcgagtc atattctgcg gagatcaaat acagcaaggc
     6541 aggtcattcc tatcatctcg ttaatccggg ccagttggat aaaaagaccc tccgccggat
     6601 gaatgaagcg ctcgcacaga atgctgaact gaaaaccggt gagtccacag ggaaggtcgt
     6661 actcgataag gataaaaaaa cagccgtgtc attatcgcta cgcatgcgga ttctaagaaa
     6721 aattgaccgt ctggctgcgc tgagtgactc gacccgaagt gaagcggtag agaagctggc
     6781 tctgcactcc gttgatgaac tgataaaaga atacagcgct aaaaagtcct gacgctaaat
     6841 ctcagaccgg ggtgaaaact ccggttttgt catatttcac ctctgtattg gcgtgatgca
     6901 aaatggcatc tgaatcgatc atggtttggc atccatttcc gatcacagtt tggcatcaca
     6961 atcgatcacg atttggcatg cttccgctca ttgattggca tcctggtgtc gttacgggga
     7021 agtcatcttc acagggatct tcagtcagtt agcattaccg acatccattc accaacttac
     7081 cggagataat caatgtcaaa accccgatgg acgcttgatc agaaaaaaca tcacgttgcc
     7141 gcatggcgcg ccagcggcct tacccgtgaa cagtactgtg aactttacga cattccgttt
     7201 aaatccctgc gccagtggcc gcaggatgtc gcaaaagccg aaaaacgagc cagagcacca
     7261 gaaattattc ctgtttcagt atctggctct tccggaatga ctgatggcag accattgtcg
     7321 gatgagcctg tcactctctt cctgcccggt ggtatccgga tgtgctgcca gccttctcag
     7381 ttaaccgatg ttttccgggc tcttcgccat gctgacgcct gataacgtat tcctggtcgt
     7441 aaaacccgtc gatatgcgcc ggggtatcga cacactgaca cagtacgtcc agaacgaact
     7501 taacgctgca tggcatgacg gtgctgcttt tgtcttcacc aataaagtcc gctcccgcat
     7561 aaaagttctc cggtgggaca aacatggcgt atggctgtgt acccgacgac tgcaccgggg
     7621 aagcttccgc tggccccgga aaggtgatgc cacatggcat ctcacacagg atgagtttca
     7681 ctggctggtc tttggggttg agtggcaaca ggtgaaaggg catgacctgg caaaatgggt
     7741 ataccaggaa gaacccgaag atgctcagag caacgcaata actcaatgaa atataatgaa
     7801 tatccatgcg tgatgtcata taatccaccg catggatatc tcgcttctca gtaccaccag
     7861 tgaccctgaa cagcttcgcg ctctggcaat tgccatggtg caaaaagtca tggctgagaa
     7921 tgcagaactt cagaaccgta tccgcatcct ggaagagcag atgaagctcg cacgccagca
     7981 gcgtttcggt aaaaaatgcg aatcgctggc cgggatgcag cgttccctgt tcgaagagga
     8041 tgttgacgcg gatatcgcag aaatctcggc gcatctggac aaactgcttc ctcagaccgg
     8101 ggacgaagaa aaaacaacaa cacgtcctgt tcgtaagccc ttgccttcac ctcttccccg
     8161 tgctgaaaag gttatcccgc cagctgagga acgttgtcct gactgtgatg cccccctgca
     8221 cttcattcgc gatgaagtca gcgagaagct ggaatatatt ccggcacagg tcgtggtcaa
     8281 ccgctatatc cgcccgcagt acagctgtcc ctgctgtgag aaagtgttca gcggaaaaat
     8341 gccggcacat atccttccga aaagtgctgt tgagccatcg gtcatcgcac aggttgtcat
     8401 cagcaaatat accgaccatc tgcccctgta tcgccagcag cacattttct cccgaatggg
     8461 ggtggagctc cccgtcagta caatggcgga tatggtcggt gttgctggcg ctgcactggc
     8521 cccgctggca aaattgttac gccacgagct tctgacacgg gatgttattc atgcagatga
     8581 aacgtccctg aggctccttg atacccggaa aggcggtaaa tcctgctccg gctggctgtg
     8641 ggcttacgtc agcggagaaa gagtaagcgt caacggagca ccgtattgac gcttatttat
     8701 tggtgagtac tacgttccat ggcaggagtt catcaactcg gttggaaggc cattctggca
     8761 gtacgctcag gatatggcgc agatacgctt ccggatcgat accgttcaga cggcaggtgc
     8821 cgatcagccc gtacaacagt gctccacgct cgccgccgtg atcgctgcca aagaacataa
     8881 agtttttctt tccgagacag actgcacgaa gcgctctttc cgcagcatta ttatccgcct
     8941 ccgccagacc gtcatcactg taataacaga gggcatccca ctgattcagt acatagctgc
     9001 acgcttcgcc cagtctggat tttttcgaca gcgtaccatt cttctccacc atccattcat
     9061 gcagtgacgt cagtaacact ttgcttcgct gctgcctgac ggcaagacgc tctgactccg
     9121 gtaatccccg tatttcatcc tcgatggcgt acagttcact gattcgcttc agggcttctt
     9181 ctgccgtcgc acttttgctg ctgatgtata catcgtggat ttttcgccgg gcatgggccc
     9241 agcacgcaac ttctgtcagt gcaccacctt cacgttctgc actgaacagc cgatcgtaac
     9301 cgctgaacgc atccgcctgc aggatacccc ggaagggacg aaggtgttgt accggatgtt
     9361 ttccctgcct gtctggtgag taggcgaacc agaccgccgg tggctctgat gagcccgcat
     9421 tccggtcatc ccggacatac gtccagatgc gtcctgtttt tgcctttttt ctgcccggtg
     9481 ccagcacttt tactggtgtg tcgtcagtgt gaaccttgcg ggtgttcatc acgtaacggt
     9541 acagggtatc attcagcaga gtcattaact gacaacatgc gtcaacccag ttggagagta
     9601 atgcccggct cagttcgatg ccctggcggg caaagatttc actctgacga tacagtggca
     9661 ggtgttcgca gtattttccc gttaacacgc gggcaagtaa tccggagccc gcgatgccgc
     9721 gctctatcgg gcgggacggc gctggcgctt caactataca gtcacatttt gtacaggctt
     9781 tttttacccg aacagtgcgg atcactttca gggcgctact caccagttcc agctgctcag
     9841 cactgacttc ccccagataa tccagctcac tgccacactc cgggcaacaa ctttcttcag
     9901 gctccagacg gtgtatttca cggggaagat gtgccggtaa cggacgacga tgacgtgact
     9961 gtcgcaactg gcggggaacc tgcgggtcat cctcacgccc actgtaacga tcgctttcct
    10021 gttcgcgttg tttcagttgg gcctcagcct gttcaacctc acgctgcagt ttttcagaac
    10081 gggtaccgaa caacatccgg cgcagttttt ctatctgggc cctcagatgt tctatttccc
    10141 gctcctcctc ttcgatcttt tcctcggcac gtgccagtgc agagcgcagg aaggcctccg
    10201 tctcttcaac cagactcagt tgctgatctt tctgacggag ggcttcagcc tgctcagaga
    10261 gcagcctttc cagctcagtg atacgaatga ggtatttccg actcatgacc gtttttataa
    10321 tccggccatg acatttttac aacattgtca gtgcattaag gcgggatgtt ttgggctgac
    10381 gccagtccag tttatcgagg agcattgcca gctgcgagcg ggtaatggat accttaccgt
    10441 cacgcaccgc aggccagata aactggcctt cctccagacg tttggtgaac aggcacagac
    10501 catcagcatc agcccacaga attttaatcg tgtcaccccg tcggccgcga aagataaaca
    10561 ggtgaccgga gaaggggttc tcatccagca catgttgtac ctgttctccc agaccgttga
    10621 aggatttacg catatcagta acgccggcaa ccagccagat tcgagtgcct gatgggagcg
    10681 agatcatcgt cccctcccgg tcagttcacg gatcaacacc gtgagcagct ctggtgaagg
    10741 attttccagc gtcatgttac cgtggcggaa ctcaacttta caggaactgg cactgactgt
    10801 gctttgtgaa ggagtggata aaagcggagt aagagccgcc ataggctctt tctgctcatc
    10861 aggcgttatc tcaacaggta ataattcaac gccagcgcca gaagaggttg ttaccggaag
    10921 acgccgcgat atacgccttt cgttctgcca gagcctgagc catttgaaca ggaggttatc
    10981 attgatatcg tgttcccggg caatacgggc aacagaggct cctggttgtg aagccagttt
    11041 aaccatttga agtttaaact catttgaaaa tgatctgcgg ggttctgagg ataatatttt
    11101 ctgttccata acaggtgtcc actagttgaa aaggtgggca cctacgttac caatactggc
    11161 ttaatggcta catacggcgg tcagtttacg cttacgagaa agaagcggtc cgccggtcgt
    11221 ctgttttgac tcgcagaccg gacgggccct cagatatcct gaggcctggc tgcagggctg
    11281 gtgtggcggt acgctggtga gtgacggata cagcgtgtac aaatctctgg cggataatga
    11341 ccccggtatt acatctgcct gttgctggag ccacgcaagg cgcggtttcg caaatctgta
    11401 taaggccagc agggagcccc gtgcagccat ggcgctgaga aaaatcgcgg gtctttatcg
    11461 cattgagaag ttcatccgtg aacgtcctgt ggaaaaaata cggcaatggc gacaacggta
    11521 ttcccggccg atagtgaatg acctgtttgc ctggcctgaa gagcaggagc cgtgctgtcc
    11581 gcccgacgga ccactaaaca aagcgattaa ttatatcctg aaccggcgcg atgaactgag
    11641 ctgcttcctg agtgacggag ccgtgcccct ggacaacaat atctgcgagc gtgctatccg
    11701 cccggtggtc atggggcgga aagcatggtt attcgccggt tcactgatgg ccgggaaccg
    11761 cgcggcacag ataatgagcc ttctggaaac ggcaaaacgt aatggtctgg agccgcatgc
    11821 ctggctgaca gacgttctga cgcgtctacc ggagtggccg gaagaccgac tggaagagtt
    11881 gctgcccctt gagggattta ccttctccgg gtgagtgata cctgccatca ggtgttcgtg
    11941 cagcgggcca taacccgcag tcgggagttg aacgcctgac gacaggaaat gagccagagc
    12001 agcgaccacg cgggctgaca gtgagtcaga acctgaatca gcccggagcc attcccgggg
    12061 ctgtacttca tcacctgcca tcagtacccg tgtcagtact tcgcgacctg acgcaattac
    12121 ccgactcagt tcttcagaac ccgcctccga taccagcctg gcggtcgccc cggcgcaaca
    12181 gaataaatca accatcgtca gactgcgatc attcgtatgc atcgctgatg atgcgcccga
    12241 aaaacgcgtc aatatgagtt cgccggaata ttactgacga cacggtttgt agttacgttc
    12301 cttcggtgag tgagtacctg tgcgtcaggg taacgcaatc agttcaggtt tagcggggtg
    12361 tcaagtcggt ttggctaacc gaccgcagaa ttaactgtac ctttaaaggt cgcccccgat
    12421 atatctgcct gtcagacata tcgccgattc ggtatctcac tacataaaac tccttaaaaa
    12481 acaatgcgat tctgttggta cgaagctttc agtaagtcag tccggagaag cgccagtacg
    12541 atacagaatg aggttgaaaa aaacgttcac gtctggtcac ttcttcaaat gttaaaaaac
    12601 atctcgtaaa gagccagttt atgcgggttc gttgagtaga cgtgcgagcg tcactttgtc
    12661 agctttaacg ctgacgaaag tgacgcccgc acagcttcaa actccacatg cacacgcaag
    12721 cgaccaaatt tcaaatggtc ttatgccgtg cgcacagtca taagaagcgc atgcggaaag
    12781 tcagaaaata tcttaagacg cagattttaa aggcgttttt atgccagtct gccgggaata
    12841 cacagtagtc cggttgtccg gaagcaccac ggtaatccga aggaaaaatc aacgtacctc
    12901 aatcaccgta gtgatatccg ttcccagagg ctaaacggat gtaccaccat aaaggcatcc
    12961 tctttcaggc tacgaggata ttgcaatcac ccgaaggcgt ttctctcaga ttgctgaaac
    13021 attggctggc agtcacccga aggcgtccct ctcagactgc tggaacattg gctggtcgca
    13081 aaagcgacag ttaatgcaaa tacaggttaa caaaaaggga aatctgccgt caacccaacc
    13141 atatgaacat gttgatttca ttatgaatgt gatgttctaa tataaattaa tcagtaactt
    13201 cataaaaaga atataagaaa cagataatgc atgtatttac acacatatca gcacaatgat
    13261 agattaccac cccagaaaca cgataaaggt ataattatgg aattttccgt tatttttttc
    13321 tgggttatca ctggtcttat taccatctta ttcttaaaat caataataac cgatctaata
    13381 aaaaac
//
LOCUS       DAZKGS010000122        13370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKGS010000122 DAZKGS010000000
VERSION     DAZKGS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(79..855)
                     /gene="yaaA"
                     /locus_tag="ACHOQY_003510"
     CDS             complement(79..855)
                     /gene="yaaA"
                     /locus_tag="ACHOQY_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9679543.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(925..2355)
                     /gene="yaaJ"
                     /locus_tag="ACHOQY_003511"
     CDS             complement(925..2355)
                     /gene="yaaJ"
                     /locus_tag="ACHOQY_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9679544.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2634..3587
                     /gene="tal"
                     /locus_tag="ACHOQY_003512"
     CDS             2634..3587
                     /gene="tal"
                     /locus_tag="ACHOQY_003512"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9679545.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3702..4289
                     /gene="mog"
                     /locus_tag="ACHOQY_003513"
     CDS             3702..4289
                     /gene="mog"
                     /locus_tag="ACHOQY_003513"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9679546.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4324..4890)
                     /gene="satP"
                     /locus_tag="ACHOQY_003514"
     CDS             complement(4324..4890)
                     /gene="satP"
                     /locus_tag="ACHOQY_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9679547.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5039..5752)
                     /gene="msyB"
                     /locus_tag="ACHOQY_003515"
     CDS             complement(5039..5752)
                     /gene="msyB"
                     /locus_tag="ACHOQY_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9679548.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5778..6182)
                     /gene="yaaI"
                     /locus_tag="ACHOQY_003516"
     CDS             complement(5778..6182)
                     /gene="yaaI"
                     /locus_tag="ACHOQY_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9679549.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6559..8475
                     /gene="dnaK"
                     /locus_tag="ACHOQY_003517"
     CDS             6559..8475
                     /gene="dnaK"
                     /locus_tag="ACHOQY_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9679550.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8564..9694
                     /gene="dnaJ"
                     /locus_tag="ACHOQY_003518"
     CDS             8564..9694
                     /gene="dnaJ"
                     /locus_tag="ACHOQY_003518"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9679551.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9798..9950)
                     /gene="mokC"
                     /locus_tag="ACHOQY_003519"
     CDS             complement(9798..9950)
                     /gene="mokC"
                     /locus_tag="ACHOQY_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9679552.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            10536..11702
                     /gene="nhaA"
                     /locus_tag="ACHOQY_003520"
     CDS             10536..11702
                     /gene="nhaA"
                     /locus_tag="ACHOQY_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9679553.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            11768..12667
                     /gene="nhaR"
                     /locus_tag="ACHOQY_003521"
     CDS             11768..12667
                     /gene="nhaR"
                     /locus_tag="ACHOQY_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9679554.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(12706..>13370)
                     /locus_tag="ACHOQY_003522"
     CDS             complement(12706..>13370)
                     /locus_tag="ACHOQY_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP9679555.1"
                     /translation="IELPGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPF
                     PLLFEIEVSSCTGNNTNCNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNE
                     NYQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIG
                     FQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVM
                     YN"
BASE COUNT         3455 a         3239 c         3403 g         3273 t
ORIGIN      
        1 attctgcaat atattgaatc tgcatgcttt tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcattga ctgcaaactt aacgctgctc gtagcgttta aacaccagtt cgccattgct
      121 ggaggaagct tcatcaaaga agtaaccttc gctattaaaa ccagtcagtt gctctggttt
      181 ggtcagccga ttttcaataa taaaacgact catcagaccg cgtgctttct tagcgtagaa
      241 gctgattatc ttaaatttgc cgttcttctc atcgaggaac accggcttga taatctcggc
      301 attcaatttc ttcggcttca ccgatttaaa atactcatct gacgccagat taatcaccac
      361 attatcgcct tgtgctgcga gcgcctcgtt cagcttgttg gtgatgatat ctccccagaa
      421 ttgatacaga tctttccctc gggcattctc aagacggatc cccatttcca gacgataagg
      481 ctgcattaaa tcgagcgggc gcagtacgcc atacaagccg gaaagcattc gcaaatgctg
      541 ttgggcaaaa tcgaaatcgt cttcgctgaa ggtttcggcc tgcaagccgg tgtagacatc
      601 acctttaaac gccagaatcg cctggcgggc attctccggc gtgaaatctg gctgccagtc
      661 atgaaagcga gcggcgttga tacccgccag tttgtcgctg atgcgcatca gcgtgctaat
      721 ctgcggaggc gtcagtttcc gcgcttcatg gatcaactgc tgggaattgt ctaacagctc
      781 cggcagcgta tagcgcgtgg tggtcaacgg gctttggtaa tcaagcgttt tcgcaggtga
      841 aataagaatc agcatatcca gtccttgcag gaaatttatg ccgactttag caaaaaaaga
      901 gaatgagttg atcgatagtt gtgattactc ctgcgaaaca tcatcccacg cgtccggaga
      961 aagctggcgg ccgatatccg gataacgcaa cggatcaaac accgggcgca cgccgagttt
     1021 acgctggcgt agataatcac tggcaatggt atgaaccaca ggcgagagca gtaaaatggc
     1081 ggtcaaattg gtaatagcca tgcaggccat tatgatatct gccagttgcc acatcagcgg
     1141 aagacttagc aaggtgccgc cgatgaccgt tgcgaaggtg cagatccgca aacaccagat
     1201 cgctttaggg ttgttcaggc gtaaaaagaa gagattgttt tcggcgtaaa tgtagttggc
     1261 aacgatggag ctgaaggcaa acagaataac cacgagggta acaaactcag caccccagga
     1321 acccattagc acccgcatcg ccttctggat aagctgaata ccttccagcg gcatgtaggt
     1381 tgtgccgtta cccgccagta atatcagcat ggcgcttgcc gtacagatga ccagggtgtc
     1441 gataaaaatg ccaatcatct ggacaatccc ttgcgctgcc ggatgcggag gccaggacgc
     1501 cgctgccgct gccgcgtttg gcgtcgaccc cattcccgcc tcattggaaa acatactgcg
     1561 ctgaaaaccg ttagtaatcg cctggcttaa ggtatatccc gccgcgccgc ctgccgcttc
     1621 ctgccagcca aaagcactct caaaaataga ccaaatgacg tggggaagtt gcccgatatt
     1681 cattacgcaa atcaccaggc tggtcagtac ccagattatc gccatcaacg ggacaaaccc
     1741 ctgcatgagc cgggcgacgc catgaagacc gcgagtgatc gccagcagag caaagacagc
     1801 gagaataata cctgtcacca gcgggggaaa atcaaaagaa aaactcaggg ctcgcgcaac
     1861 ggcgttcgcc tgaattccgc tgaaaattat gccgtaggcg atgagcaaaa agacggcgaa
     1921 cagaacgccc atccagcgca tccccagccc gcgcgccata taccatgccg gtccgccacg
     1981 aaactgccca ttggcatcac gttctttata aagctgtgcg agggaacatt cggcaaacga
     2041 ggtcgccatg ccgataaacg cggcaaccca catccagaag acggctccag gtccaccggc
     2101 ggtaattgcc agcgcaacgc cggccaggtt gccgctaccc acgcgcgccg caagactggt
     2161 acacaatgac tgaaatgagg ttaaaccgcc tggctgtgga tgaatgctat ttttaagact
     2221 tttgccaaac tggcggatgt agcgaaactg cacaaatccg gtgcgaaaag tgaaccaaca
     2281 acctgcgccg aagagcaggt aaatcattac cgatccccaa aggacgctgt taataaagga
     2341 gaaaaaatct ggcatgcata tccctcttat tgccggtcgc gatgactttc ctgtgtaaac
     2401 gttaccaatt gtttaagaag tatatacgct acgaggtact tgataacttc tgcgtagcat
     2461 acatgaggtt ttgtataaaa atggcgggcg atatcaacgc agtgtcagaa atccgaaaca
     2521 gtctcgcctg gcgataaccg tcttgtcggc ggttgcgctg acgttgcgtc gtgatatcat
     2581 cagggcagac cggttacatc cccctaacaa gctgtttaaa gagaaatact atcatgacgg
     2641 acaaattgac ctcccttcgt cagtacacca ccgtagtggc cgacactggg gacatcgcgg
     2701 caatgaagct gtatcaaccg caggatgcca caaccaaccc ttctctcatt cttaacgcag
     2761 cgcagattcc ggaataccgt aagttgattg atgatgctgt cgcctgggcg aaacagcaga
     2821 gcaacgatcg cgcgcagcag atcgtggacg cgaccgacaa actggcagta aatattggtc
     2881 tggaaatcct gaaactggtt ccgggccgta tctcaactga agttgatgcg cgtctttcct
     2941 atgacaccga agcgtcaatt gcgaaagcaa aacgcctgat caaactctac aacgatgctg
     3001 gtattagcaa cgatcgtatt ctgatcaaac tggcttctac ctggcagggt atccgtgctg
     3061 cagaacagct ggaaaaagaa ggcatcaact gtaacctgac cctgctgttc tccttcgctc
     3121 aggctcgtgc ttgtgcggaa gcgggcgtgt tcctgatctc gccgtttgtt ggccgtattc
     3181 ttgactggta caaagcgaat accgataaga aagagtacgc tccggcagaa gatccgggcg
     3241 tggtttctgt atctgaaatc taccagtact acaaagagca cggttatgaa accgtggtta
     3301 tgggcgcaag cttccgtaac atcggcgaaa ttctggaact ggcaggctgc gaccgtctta
     3361 ccatcgcacc tacactgctg aaagagctgg ctgagagcga aggggctatc gaacgtaaac
     3421 tgtcttacac cggcgaagtg aaagcgcgtc cggcgcgtat cactgagtcc gagttcctgt
     3481 ggcagcacaa ccaggatcca atggcagtag ataaactggc ggaaggtatc cgtaagtttg
     3541 ctattgacca ggaaaaactg gaaaaaatga tcggcgatct gctgtaatca ttcttagcgt
     3601 gaccgggaag tcggtcacgc tacctcttct gaagcctgtc tgtcactccc ttcgcagtgt
     3661 atcattctgt ttaacgagac tgtttaaacg gaaaaatctt gatgaatact ttacgtattg
     3721 gcttagtttc catctctgat cgcgcatcca gcggcgttta tcaggataaa ggcatccctg
     3781 cgctggaaga atggctgaca tcggcgctaa ccacgccgtt tgaactggaa acccgcttaa
     3841 tccccgatga gcaggcgatc atcgagcaaa cgttgtgtga gctagtggat gaaatgagtt
     3901 gccatctggt gctcaccacg ggcggaactg gcccggcgcg tcgtgacgta acgcccgatg
     3961 cgacgctggc agtagcggac cgcgagatgc ctggctttgg tgaacagatg cgccagataa
     4021 gcctgcattt tgtaccaact gcgatccttt cgcgtcaggt tggtgtgatt cgcaaacagg
     4081 cgctgatcct taacttaccc ggtcagccga agtctattaa agagacgctg gaaggtgtga
     4141 aggacgctgc gggtaacgtt gtggtacacg gtatttttgc cagcgtaccg tactgcattc
     4201 agttgctgga agggccatac gttgaaacgg caccagaagt ggttgctgca ttcagaccga
     4261 agagtgcaag acgcgacgtt agcgaataaa aaaatccccc cgagcggggg gatctcaaaa
     4321 caattagtgg gattcaccaa tcggcagaac ggtgcgacca aactgctcgt tcagtacttc
     4381 acccatcgcc agatagattg cactggcacc acagatcaga ccaatccagc cggcaaagtg
     4441 gatgattgcg gcgttaccgg caatgttacc gatcgccagc agggcaaaca gcacagtcag
     4501 gctaaagaaa acgaattgca gaacgcgtgc gcctttcagc gtgccgaaga acataaacag
     4561 cgtaaatacg ccccacagac ccaggtagac accaaggaac tgtgcatttg gcgcatcggt
     4621 cagacccagt ttcggcatca gcagaatcgc aaccaacgtc agccagaaag aaccgtaaga
     4681 ggtgaatgcg gttaaaccga aagtgttgcc ttttttgtac tccagcagac cggcaaaaat
     4741 ttgcgcgatg ccgccgtaga aaatgcccat ggcaagaata ataccgtcca gagcgaaata
     4801 acccacgttg tgcaggttaa gcagaatggt ggtcatgccg aagcccatca ggcccagcgg
     4861 tgccggatta gccaacttag tgttgcccat aattcctcaa aaatcatcat cgaatgaatg
     4921 gtgaaataat ttccctgaat aactgtagtg ttttcagggc gcggcataat aatcagccag
     4981 tggggcagtg tctacgatct tttgagggga aaatgaaaat tttccccggt ttccggtatc
     5041 agacctgagt ggcgctaacc atccggcgca ggcaggcgat ttgcagtacg gctggaatcg
     5101 tcacgcgata ggcgctgccg ctgaccgctt taaccccatt tagtgccgca cctacagggc
     5161 ctcccagccc cgcgccgcgc agcaaaccat gcccaagtac gctcattgct gcgtgggtgc
     5221 gtaaaatgcg ggtcagttgg ctggaaagca aatgcgacac gccttttgcc aataatttgt
     5281 ctttcatcag cagcggcagc agctcttcca gctcattcac cctcgcatcg accgcgtgca
     5341 gaaactcctg tttatgttcc tcgtccatat tcttccaggt gttacgcaga aattgttcca
     5401 gtaactgttg ctcaatctca aacgtagaca tctctttgtc ggctttcagc ttcaatcgtt
     5461 ttgaaacatc gagcaaaatg gcccgataca atttaccgtg tccgcgcagt ttgttggcga
     5521 tactatcgcc accaaaatgc tgtaattctc cagcaatcag ctgccagttg cggcgatgtt
     5581 gctcgggatg cccttccata gatttaaaca gttcgttgcg catcagtacg ctggagaggc
     5641 gagttttgcc tttttcatta tgggtgagca atcgggcgaa atttgccaac tgttcctcac
     5701 tgcaatgctg gagaaaatcc agatctgaat cattcaggta attaacattc attttttgtg
     5761 gcttctatat tctggcgtta gtcgtcgccg ataattttca gcgtggccat atccgatgag
     5821 ttcaccgtat gaccggaaaa ggtgattttt gagacgcagc gtttattgtc gttatcgctg
     5881 ttaatgttga tccagtcagt ggtttgccct tcttttattt ctgaaggaat attcaggctc
     5941 tgactggcgc tacgggcggc tttgaaataa accgatgcac cgcttaactg taaatcgcca
     6001 tggtcggcag agagttgtat gcgtttaaca attcggcaaa caggaagttt cagcgccaga
     6061 tcgttggttt cgttacgtgg catcgcaatg acgccgagga ttttatggtc gtttgcctgc
     6121 gccgtgcagc acagcatcag actaatcgcc aggctggcgg aaatcgtaaa aacggatttc
     6181 ataaggattc tcttagtggg gagaggtagg gggatggata cccactagtt tactgctgat
     6241 aaagagaaga ttcaggtgcg taatgttttc tttttattac aattttttga tgaatgtctt
     6301 ggctgcgatt cattctttat atgaataaaa ttgctgtcag tttcacgtct tgtcctgcta
     6361 tatcgcgaaa tttctgcgca aaagcacaaa aaatttttgc atctccccct tgatgacgtg
     6421 ggttacgacc ccatttagta gtcaaccgca gtgagtgagt ctgcaaaaaa atgaaattgg
     6481 gcagttgaaa ccagacgttt cgcccctatt acagactcac aaccacatga tgaccgaata
     6541 tatagtggag acgtttagat gggtaaaata attggtatcg acctgggtac taccaactct
     6601 tgtgtagcga ttatggatgg caccactcct cgcgtgctgg agaacgccga aggcgatcgc
     6661 accacgcctt ctattattgc ctatacccag gatggtgaaa ctctggttgg tcagccggct
     6721 aaacgtcagg cagtgacgaa cccgcaaaac accctgtttg cgattaaacg cctgattggc
     6781 cgccgcttcc aggacgaaga agtacagcgt gatgtttcca tcatgccgtt caaaattatt
     6841 gctgctgata acggcgacgc atgggtcgaa gttaaaggcc agaaaatggc accgccgcag
     6901 atttctgctg aagtgctgaa aaaaatgaag aaaaccgctg aagattactt gggtgaaccg
     6961 gtaactgaag ctgttatcac cgtaccggca tactttaacg atgctcagcg tcaggcaacc
     7021 aaagacgcag gccgtatcgc tggtctggaa gtaaaacgta tcatcaacga accgaccgca
     7081 gctgcgctgg cttacggtct ggacaaaggt actggcaacc gtactatcgc ggtttatgac
     7141 ctggggggtg gtactttcga tatttctatt atcgaaatcg acgaagttga cggcgaaaaa
     7201 accttcgaag ttctggctac caacggtgat acccacctgg gtggtgaaga cttcgacagc
     7261 cgtctgatca actacctggt tgaagaattc aagaaagatc agggcattga cctgcgcaac
     7321 gatccgctgg caatgcagcg cctgaaagaa gcggcagaaa aagcgaaaat cgaactgtct
     7381 tccgctcagc agaccgacgt taacctgccg tacatcactg cagatgcgac cggtccgaaa
     7441 cacatgaaca tcaaagtgac tcgtgcgaaa ctggaaagcc tggttgaaga tctggtaaac
     7501 cgttccattg agccgctgaa agttgcactg caggacgctg gcctgtccgt atctgatatc
     7561 gacgacgtta tcctcgttgg tggtcagact cgtatgccaa tggttcagaa gaaagttgct
     7621 gagttctttg gtaaagagcc gcgtaaagac gttaacccgg acgaagctgt agcaatcggt
     7681 gctgctgttc agggtggtgt tctgactggt gacgtgaaag acgtactgct gctggacgtt
     7741 accccgctgt ctctgggtat cgaaaccatg ggcggtgtga tgacgacgct gatcgcgaaa
     7801 aacaccacta tcccgaccaa gcacagccag gtgttctcta ccgctgaaga caaccagtct
     7861 gcggtaacca tccatgtgct gcagggtgaa cgtaaacgtg cggctgataa caaatctctg
     7921 ggtcagttca acctggatgg tatcaacccg gcaccgcgcg gcatgccgca gatcgaagtt
     7981 accttcgata tcgatgctga cggtatcctg cacgtttccg cgaaagataa aaacagcggt
     8041 aaagagcaga agatcaccat caaggcttct tctggtctga acgaagatga aatccagaaa
     8101 atggtacgcg acgcagaagc taacgccgaa gctgaccgta agtttgaaga gctggtacag
     8161 actcgcaacc agggcgacca tctgctgcac agcacccgta agcaggttga agaagcaggc
     8221 gacaaactgc cggctgacga caaaactgct atcgagtctg cactgactgc actggaaact
     8281 gctctgaaag gtgaagacaa agccgctatc gaagcgaaaa tgcaggagct ggcacaggtt
     8341 tcccagaaac tgatggaaat cgcccagcag caacatgccc agcagcagac tgccggtgct
     8401 gatgcttctg caaacaacgc gaaagatgac gatgttgtcg acgctgaatt tgaagaagtc
     8461 aaagacaaaa aataatcgcc ctataaacgg gtaattatac tgacacgggc gaaggggaat
     8521 ttcctctccg cccgtgcatt catctagggg caatttaaaa aagatggcta agcaagatta
     8581 ttacgagatt ttaggcgttt ccaaaacagc ggaagagcgt gaaatcaaaa aggcctacaa
     8641 acgcctggcc atgaaatacc acccggaccg taaccagggt gacaaagagg ccgaggcgaa
     8701 atttaaagag atcaaggaag cttatgaagt tctgaccgac tcgcaaaaac gtgcggcata
     8761 cgatcagtat ggtcatgctg cgtttgagca aggtggtatg ggcggcggcg gttttggcgg
     8821 cggcgcagac ttcagcgata tttttggtga cgttttcggc gatatttttg gcggcggacg
     8881 tggtcgtcaa cgtgcggcgc gcggtgctga tttacgctat aacatggagc tcaccctcga
     8941 agaagctgta cgtggcgtga ccaaagagat ccgcattccg actctggaag agtgtgacgt
     9001 ttgccacggt agcggtgcaa aaccaggtac acagccgcag acctgtccga cctgtcatgg
     9061 ttctggtcag gtgcagatgc gtcagggatt cttcgctgta cagcagacct gtccacactg
     9121 tcagggccgc ggtacgctga tcaaagatcc gtgcaacaaa tgtcatggtc atggtcgtgt
     9181 tgagcgcagc aaaacgctgt ccgttaaaat cccggcaggg gtggacactg gagaccgcat
     9241 ccgtcttgcg ggcgaaggtg aagcgggcga gcatggcgca ccggcaggcg atctgtacgt
     9301 tcaggttcag gttaaacagc acccgatttt cgagcgtgaa ggcaacaacc tctattgcga
     9361 agtcccgatc aacttcgcta tggcggcgct gggtggcgaa atcgaagtac cgacccttga
     9421 tggtcgcgtc aaactgaaag tgcctggcga aacccagacc ggtaagcttt tccgtatgcg
     9481 cggtaaaggc gtcaagtctg tccgcggtgg cgcacagggt gatttgctgt gccgcgttgt
     9541 cgtcgaaaca ccggtaggcc tgaacgagaa gcagaaacag ctgctgcaag agctgcaaga
     9601 aagcttcggt ggcccaaccg gtgagcacaa cagcccgcgc tcaaagagct tctttgatgg
     9661 tgtgaagaag ttttttgacg acctgacccg ctaacctccc caaaagcctg cccgtgggca
     9721 ggcttgggta aaattagggt gcgttgaaga tatgcgagca cctgaaaagt ggcggggatc
     9781 actccccgcc gttgcactta ctcggattcg taagccgtga aaacagcaac ctccgtctgg
     9841 ccagttcgga tgtgaacctc acagaggtct tttctcgtta ccagcgccgc cactacggcg
     9901 gtgatacaga tgacgatcag ggcgacaatc atcgccttat gctgcttcat tgctctcttc
     9961 tccttgacct tacggtcagt aagaggcact ctacatgtgt tcagcatata gggggcctcg
    10021 ggttgatagt aaaatatcac tcggggcttt tctctatctg ccgttcagct aatgcctgag
    10081 acagatagcc tcaagtaccc gccgctatta tatcgctctc tttaacccat tctgttttat
    10141 cgattctaat cctgaagacg cctcgcattt ttgtggcgta attttttaat gatttaatta
    10201 tttaacttta atttatctct ttatcgcaat tattgacgac aagctggatt atttttgaaa
    10261 tattgtccta acaaataacg ccgactgaca agaaattaat tattactttt cctaattaat
    10321 ccctcagaaa tcctcacctt aagctatgat tatctgggct tagggtcact cgtgagcgct
    10381 tacagccgtc aaaaactcat ctcaccgctg atggcgcaaa ttcttcaatg gatcgtaaaa
    10441 aacgagttat tcctacacta taatctgatt ttaacgatga tttgtgcggg gtaaaatagt
    10501 aaaaacgatc tattcacctg aaagagaaat aaaaagtgaa acatctgcat cgattcttta
    10561 gcagtgatgc ctcgggaggc attattctta tcattgccgc tatcctggcg atgattatgg
    10621 ccaatagcgg cgcaaccagt ggatggtatc acgactttct ggagacgccg gttcagctcc
    10681 gggttggttc actcgaaatc aacaaaaaca tgctgttatg gataaatgac gcgctgatgg
    10741 cggtattttt cctgttagtc ggtctggaag ttaaacgtga actgatgcaa ggatcgctag
    10801 ccagcttacg ccaggccgca tttccagtta tcgccgctat tggtgggatg attgttccgg
    10861 cattactcta tctggccttt aactatgccg atccgattac ccgcgaaggg tgggcgatcc
    10921 cggcggctac tgacattgcc tttgcacttg gtgtgctggc gctgttggga agtcgtgttc
    10981 cgttagcgct gaagatcttt ttgatggctc tggctattat cgacgatctt ggggccatca
    11041 ttatcatcgc attgttctac actaatgact tatcgatggc ctctcttggc gtcgcggctg
    11101 tagcaattgc ggtactcgcg gtattgaatc tgtgtggtgt acgccgcacg ggcgtctata
    11161 ttctggttgg cgtagtgttg tggactgcgg tgttgaaatc gggggttcac gcaaccctgg
    11221 cgggggtaat tgtcggcttc tttattcctt tgaaagagaa gcatgggcgt tctccagcga
    11281 agcgactgga gcatgtgtta cacccgtggg tggcgtatct gattttgccg ctgtttgcat
    11341 ttgctaatgc tggcgtttca ctgcaaggtg tcacgctgga tggcttgacc tccattctgc
    11401 cattggggat catcgctggc ttgctgattg gcaaaccgct ggggattagt ctgttctgct
    11461 ggttggcgct gcgtttgaaa ctggcgcatc tgcctgaggg aacgacttat cagcaaatta
    11521 tggcggtggg gatcctgtgc ggtatcggtt ttactatgtc tatctttatt gccagcctgg
    11581 cctttggtag cgtagatcca gaactgatta actgggcgaa actcggtatc ctggtcggtt
    11641 ctatctcttc ggcggtaatt ggatacagct ggttacgcgt tcgtttgcgt ccatcagttt
    11701 gacaggacgg tttaccgggg agccataaac ggctcccttt tcattgttat cagggagaga
    11761 aatgagcatg tctcatatca attacaacca cttgtattac ttctggcatg tctataaaga
    11821 aggttccgtg gttggcgcag cggaggcgct ttatttaact ccacaaacca ttaccggaca
    11881 gattcgagcg ctggaagagc gcctgcaagg caaattattt aaacgcaagg gacgtggtct
    11941 cgaacccagc gagctgggag aactggtcta tcgctatgcc gataaaatgt tcaccttaag
    12001 ccaggaaatg ctggatattg tgaactatcg caaagaatcc aatttattgt ttgacgttgg
    12061 tgtggctgat gcactttcca aacgcctggt cagtagcgta cttaacgccg cagtggtaga
    12121 aggcgagccc attcatcttc gctgcttcga atccacccac gaaatgctgc tggagcaatt
    12181 aagtcagcat aaactggata tgatcatttc tgactgtccg atagactcta cgcagcagga
    12241 aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt ttctggtgta caaatccacc
    12301 accagaaaaa ccgttcccgg cttgtctgga agaacggcga cttttgattc ctgggcgacg
    12361 ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc cagggattaa acgtagaaat
    12421 cctcggcgag tttgatgatg ccgcgttgat gaaagctttt ggtgcgatgc acaatgcaat
    12481 cttcgttgcc ccaacgcttt atgcatatga cttttatgcc gataaaactg tcgtagaaat
    12541 tggtcgcgtc gagaatgtga tggaagagta ccatgctatt tttgctgagc ggatgattca
    12601 gcacccggcg gtacagcgaa tctgcaatac ggattattct gcgcttttta gtccagcggc
    12661 gcgttaatcg gcacctcccc ccaaagttaa ggtgggggag atagattagt tgtacataac
    12721 cacgattttg actcggctca ttatttgccc gcttgagaca ttatttccat atgtacgcgg
    12781 gcgaataaat agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt
    12841 gcttttacca tactggattt cattcgtacc atcattggag agttgaaagc ctatttgacc
    12901 cgcaccgtcc tcactgtttt ctaactcatt ttttaatatg ctggttcctg actcctgtat
    12961 cgtcactggt tcaaagtaat attcaacatc actgggttca aaaactaatt caccttgctc
    13021 gccctggatg cagtcaaatc ttatatgatg aaactgataa ttttcattat tgatacgaga
    13081 cagtacaaat cgtccagtgt cgatggattg atcttcaaaa ccacaggtca ttaaatttac
    13141 ctgtaaaata gtatttaaat tataattaaa gcggacgcta ttacaattag tattattccc
    13201 tgtacatgaa ctcacttcaa tttcgaacaa caaaggaaaa ggagttccag gaaaaatgtc
    13261 tgcggtgtta gaactatctg atacagatgc gatagaatat ggaatggtga aatttatcgc
    13321 agatagcatt tcttctaaat tataagatga cttccctgga agctctattt
//
LOCUS       DAZKGS010000123        13316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKGS010000123 DAZKGS010000000
VERSION     DAZKGS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..528
                     /locus_tag="ACHOQY_003523"
     CDS             <1..528
                     /locus_tag="ACHOQY_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9679556.1"
                     /translation="EVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAIL
                     QNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNT
                     RLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNIN
                     AVAMKITADIMQHAD"
     gene            complement(682..2331)
                     /gene="yjcE"
                     /locus_tag="ACHOQY_003524"
     CDS             complement(682..2331)
                     /gene="yjcE"
                     /locus_tag="ACHOQY_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9679557.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2483..3832)
                     /gene="ghxP"
                     /locus_tag="ACHOQY_003525"
                     /gene_synonym="yjcD"
     CDS             complement(2483..3832)
                     /gene="ghxP"
                     /locus_tag="ACHOQY_003525"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9679558.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4378..4842)
                     /gene="soxR"
                     /locus_tag="ACHOQY_003526"
     CDS             complement(4378..4842)
                     /gene="soxR"
                     /locus_tag="ACHOQY_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9679559.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4928..5251
                     /gene="soxS"
                     /locus_tag="ACHOQY_003527"
     CDS             4928..5251
                     /gene="soxS"
                     /locus_tag="ACHOQY_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9679560.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5254..6840)
                     /gene="pdeC"
                     /locus_tag="ACHOQY_003528"
     CDS             complement(5254..6840)
                     /gene="pdeC"
                     /locus_tag="ACHOQY_003528"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9679561.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7270..7551
                     /gene="yjcB"
                     /locus_tag="ACHOQY_003529"
     CDS             7270..7551
                     /gene="yjcB"
                     /locus_tag="ACHOQY_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9679562.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(7650..8186)
                     /gene="ssb1"
                     /locus_tag="ACHOQY_003530"
     CDS             complement(7650..8186)
                     /gene="ssb1"
                     /locus_tag="ACHOQY_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9679563.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8440..11262
                     /gene="uvrA"
                     /locus_tag="ACHOQY_003531"
     CDS             8440..11262
                     /gene="uvrA"
                     /locus_tag="ACHOQY_003531"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9679564.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11297..11653)
                     /gene="yjbR"
                     /locus_tag="ACHOQY_003532"
     CDS             complement(11297..11653)
                     /gene="yjbR"
                     /locus_tag="ACHOQY_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9679565.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11657..12073)
                     /gene="yjbQ"
                     /locus_tag="ACHOQY_003533"
     CDS             complement(11657..12073)
                     /gene="yjbQ"
                     /locus_tag="ACHOQY_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9679566.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12184..12897)
                     /gene="aphA"
                     /locus_tag="ACHOQY_003534"
     CDS             complement(12184..12897)
                     /gene="aphA"
                     /locus_tag="ACHOQY_003534"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9679567.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3468 a         3466 c         3242 g         3140 t
ORIGIN      
        1 gaggtaaatc tttataaagt aatagcgcca tatgttaatt tatttaaagc cgaccttagt
       61 ttctctaaac tcgatttaat taactttgaa catgctgatc tgtctcgcgt caatctgaac
      121 aaagcaatcc tccagaatat aaacttaatt gatagcaaac tcttttgtac gtggctaaca
      181 aatacattcc tcgaaatggt tatatgtacc ggctctaata tggctaatgt taattttaat
      241 aatgccaatt taagcaactg ccatttcaac tgttctattt taacaaaagc ctgtatgttt
      301 aatacccgtc tctatcgggt taattttgat gaggctagcg tccagggaat gggcatttct
      361 attctccgtg gggaggaaaa tatccccatt gatagtgata ccctggtaac actacagaaa
      421 ttctttgaag aagattgtac ctctcatact ggcatgtcac aaactgagga taatattaat
      481 gcagtcgcta tgaagattac tgcagatatt atgcaacacg cagattgagg ttaagtcgaa
      541 gaacagccaa tgaattgact ttacaagaga gataacttat ttgttttaga atcatgaatg
      601 ttagcccgag ccaacctgtt aaaggtgcaa aagattgcac ctttttcaga ttacctcggg
      661 cctggttacg tcgctttcgg cttactgatt ttcctcaatt agcaacgctt caagcaaatc
      721 gagatcgtgc agtaattttt gcagcgtttc gttgctgatc tcccgcgtgg cgcgcaggtg
      781 gtacagttca gcacgttcag aacgcaatgc cgccagacgg aagcgacgct caaggttctc
      841 ttcctgcacg gaactttcaa cgtcgttacg tccatcggcg cgacgacgca ggttaccaat
      901 gacgcgagaa ctgacctccg taagcagctg gttatcgata ttctcttcgg tatccgccgc
      961 cagacgctcc tccattttct ggatcgccac aatcgccact tctgccgttg ccgctcgcgc
     1021 aatacgttct tctttcaatt gttgcgaatg gtccgcgact tcaatgtgtt gtagcagaat
     1081 aggcaacatc accacgccga caaacagcga gaagagaatg acgccagccg ccaggaacac
     1141 cagctcatag cgcgccggga agacgttacc atccggcaag agcagcggga tagagagcac
     1201 accggccaga gtgatcgccc cacgcacccc ggcgaaagac gcgatcagga tttctcgtgt
     1261 ggtccacgaa ccaaactcca tcggcttctt cttcaggaag cggttgctga actttttcat
     1321 cgtccacagc cagccgaaac ggaccagcat cagcgccgca tatatcagaa taatatcggt
     1381 aaacagcatc cagatttcga cgttagggtc gatttctgcc gccatcagcg acgtctccag
     1441 aatacccggc agctgcagac ctaacagcag gaacaccatg ccgttaaaga caaattccag
     1501 catcgcccag gtgctgtttg cgcgcaggcg cattgccagc ggcgcacggc gcatcacacc
     1561 ggagcgggtg atggtcatcc cggcggcaac cgcagcgagg atgccggaaa cgccaatatg
     1621 ttcggcaatc agataagaag cgaatggcag caagaacagc agaacgatct gcgtcgcggg
     1681 ttcatcaccg ccccagcggc tgaggaatcg cagcgagcga ccatacagcc agctcaccac
     1741 aaagccggcg agaataccgc caatggctac tttcataaat tcgaccgtcg caccgccaac
     1801 ggtgaagatc atcgtcccca tcgccactgc cacggcaaac ttcaacgaca ccaggccgga
     1861 ggcgtcattc atcaacgctt cgccctgcaa aatacccatg atttttttcg ggatgcgccc
     1921 ttcgccaaca atcccggaga gcgccacagc atcggtcgga gaaagcactg ccgccagcgc
     1981 aaaggcgggg atcagcggaa tgcccggcac cacccagtaa ataaggaagc caatgccgac
     2041 cacggtgacc accaccagcg ccagcgcgag gccaaaaatc tctcgaccat gttcgagaaa
     2101 ttcacgggtc ggcgttttcc agccatcagc gaacagcaac ggcgggataa acaagactaa
     2161 aaagagttca ggatcaaact ccacatgcaa accaaacgtc ggccacgcca gtagcgcacc
     2221 gatggcgatt tgcataagcg gaagcgggat ctgaaagggc atgacacgag tgaccacccc
     2281 ggacagcgag accacgaggg tcattatcag tatggtgaag aagatttcca tgcgttccct
     2341 gtttgcgatg tttttttatg atctgagaaa gacgttatgc ctgaattctc attttttcag
     2401 aatatcgcaa aagctcacac aatgtgcgga gaaaataaaa acgggcagcc attgccaccc
     2461 gttttcagaa tccgatgaaa gattagatag cccagccacc cgcatagaag gtcaccagcg
     2521 ccacggcgat aacgaccgta ccgatgttca acttgcgcca ttcgccggaa accagacgac
     2581 caatcaccag agtcgcgaag ccgatcatga tgcctgttac gatgttacag gtcagcacga
     2641 tgaataccgc cgtaaccaga cccgccatcg catcaacaaa atcagcaaag tcgattttcg
     2701 ccacgttgct cagcatcagc aggccaacgt acatcagcgc cggagccgtt gcataccccg
     2761 gaacgaggta agagagcgga gagaggaaca gaatcagcag gaacagcacg ccaacggtga
     2821 tagccgtcaa accggtttta ccgcccgccg ccgtacccgc cgcagactcg atgtataccg
     2881 ctgccggagc agcacccacc aggccagaga aaacgctgct catggagtca gtggtcagtg
     2941 ctttcccacc gtcgatgatc tgcccatctt tatccagcag gttcgcctgg ccggcgacgg
     3001 cacggatagt tccggtggca tcaaatactg ccgtcatcac cagcgccaga acgcttggca
     3061 ggactacagg attcagcgcg cccataatgt ccaggctgcc aatcagtgaa ttgccgtttt
     3121 catcgctcaa tgaaggcatg gcgaaaacgc cggagaaatg gacgttagga tcgaagatca
     3181 aaccgacaat tgagataccg ataatggtca gcagaatgcc accagggact ttcagttttt
     3241 ccaggccgat gatcaccgcc agacctacta gtgacataat caccgggaag gtcgcgaaat
     3301 cacccagcgc aacgggcaga ccatcaagcg ggtttttaat caccagaccg acaccattag
     3361 cggcaatgag cagcaggaac agaccgatac cgatccccgt gccgtgcgcc acaccgtgcg
     3421 gcaagttgcg caaaatccag ctacggatac ccgtggcaga aattaccgta aacagcaccc
     3481 ccatcaggaa cacggcaccc agcgcgacag gtacgctaat atgttgcccc agcaccaggc
     3541 tgaatgcggt aaacgccgtc agggagatgg cgcaaccaat cgccaacggc agattagccc
     3601 acagacccat cacgatagaa ccgagtccgg caaccagaca ggttgcaacg aaaactgccg
     3661 caggcgggaa gcctgcttta cccaacatac ctggaacgac gatgaccgag tagaccatcg
     3721 ccagaaacgt tgttaacccg gcaaccactt cctgacggac agtgcttcca cgttgtgaaa
     3781 ttttaaacca ggcgtcgagt gaaccgccgg tacgcgctga tggcgtagac ataagaaaac
     3841 atcccctgag agtttaattt tcgtcaagat gcgtggcgga caatcccctg ccagcgaaac
     3901 gttatcttcc tgctccaggt ttgcgacaat ttactgcgtc gcaaaaaagc aaacgtttag
     3961 ctccgcgcat acaaaacggg tggtaaaaaa agcaaacgat tatccggcga tcatagcggc
     4021 tttttcaacc gaagtttgct gcttttttct cttttattgt cttgacgatg aaaaaccagg
     4081 caattgatgc gcaaacgtta tcgtcggtgc gcaagattgc agcgttatcg agccgtatac
     4141 cgttcaggat tgcgataaaa aatcgtctga gatgacaaaa atattgcgga taaaaagtat
     4201 acttttacac tggcgaagaa atgccggtga catacaatta aagcatcaac accaaccgga
     4261 acctccacca cgtgctcgaa tggggtgtgt tgacgtcggg ggaaaccctc ctgtgtacca
     4321 gcgggataga gagaaagaca aagaccggaa aacaaactaa agcgcccttg tggcgcttta
     4381 gttttgttca tcttccagca agcgtgcgcc ggtaccttct tctcctaagc ggtcgcccgg
     4441 gttacgcaac gggcaatcac tgcgcgaaag gcagccacaa ccaatacatc cgtccagttc
     4501 gtcacgcagc gctactaagg tatgaatgcg ccgatccaac tcttctcgcc attgggacga
     4561 aagctgtttc cactctttcg cacttaacgt atgcccttcg ggcaacacgc caaacgcttc
     4621 accaatggtc gccagcggaa tgccaatacg ctgagcaatt ttgataattg caacatatcg
     4681 caacacatca cgtttatatc gccgctgatt gccgctgtta cggatactgg taatcaaccc
     4741 tttactttca tagaaatgca gcgccgatac cgccacaccg ctgcgtttcg ccacttcgcc
     4801 gggggttagc agcgctttaa tgcggggtaa tttcttttcc ataaatcgct ttacctcaag
     4861 ttaacttgag gaattatact ccccaacaga tgaattaacg aactgaacac tgaaaagagg
     4921 cagatttatg tcccatcaga aaattattca ggatcttatc gcatggattg acgagcatat
     4981 tgaccagccg cttaacattg atgtagtcgc aaaaaaatca ggctattcaa agtggtactt
     5041 gcaacgaatg ttccgcacgg tgacgcatca gacgcttggc gattacattc gccaacgtcg
     5101 cctgttactg gccgccgttg agttgcgcac caccgagcgt ccgatttttg atatcgcaat
     5161 ggacctgggt tatgtctcgc agcagacctt ctcccgcgtt ttccgtcggc agtttgatcg
     5221 cactcccagc gattatcgcc accgcctgta gttttattgc ccgcgcgtta actcccgcgc
     5281 gggtaattgc tccatccatt gcataaacac ctgcggcggc atcgccttcg caaaaaaaca
     5341 tccctggcaa tagcgcacgc cgcgtttgcg cagccagtta acctgctcct cagtttcgac
     5401 gccttcagcg atcgttttta accccaggct gtgcgccagc tcgatgatgt gttccgcaat
     5461 caaatgactg gttttgtggg tggtcagcgt ttcaacaaac gatttatcga ttttcaaaat
     5521 atcgacattc aatgatttaa ggttatgcaa gttagagtag ccaataccaa aatcatcaat
     5581 tgccacttcg taaccagcct ggcggaaagc cagaataatc ggcgtcattt tgtcaacatc
     5641 aagaaatgca tgttcagtca cttcaaattt aatttgctgc ggacgcaccg cgtattgctc
     5701 tgttttctga ttgattcgcg ctatcaaccg tgacgtatga aaatcggagg ccgacaggtt
     5761 aatagaaaca tagcgatcgg catgtgttgc caagtacgcg cccagatcac ggaagacatt
     5821 atcaataaca taatcagtta tctgttctat catcccctcc ttttctgcca gcggaataaa
     5881 ctctgccgga ttcattattt gcccctgctc accaagccaa cgtaacaacg cttcagcgcc
     5941 gatacatttt tctgttttga tatcgattat tggctggtaa taaagacaaa gttgatgttt
     6001 ttcgagggcg cgttgcaatt tacgtttggg agataaatag ttttgtcgaa tacgtagcca
     6061 gagtaatagc agaacaagac tccccaaaat accggcgggc aacgcaaaaa taagatgatt
     6121 ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa ttgggcgttt
     6181 tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat ccttcaaatg
     6241 aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa aggttttcgt
     6301 caccgtatca tacacacccc attgcaatgt cggatcatca gacatcactt cactccagaa
     6361 gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt tatagccaga
     6421 gaaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct tataatcggc
     6481 cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa aatggttatc
     6541 acgggcatag atcaattcat taatataaag atagccacga ataatattca acattcgctt
     6601 ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc tcactaaatc
     6661 tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg ttcggagctg
     6721 attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa
     6781 gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat
     6841 tgctatcaat ttcctgcctg tttatacccg gccattgcaa ggtgaaaacc ctgggtaccg
     6901 acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga aattgatacc
     6961 tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat ttcctacagg
     7021 aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca taatctgtac
     7081 agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata tagttcacaa
     7141 aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc gatagccaga
     7201 gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc ttttcagagg
     7261 aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca acttcttagt
     7321 gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc tgattatgtc
     7381 gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt cgcaatgggg
     7441 ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga gttggtgatg
     7501 atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg atacgtagaa
     7561 gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat ccaccttaaa
     7621 acaatataac ctattgtttt aatgacaaat cagaacggaa tgtcatcatc aaagtccatc
     7681 ggcggctcgt tagacggcgc tgccggagcg gactgctgcg ggcgagactg cgcgccgccg
     7741 ctgaactgat tgccaccctg cggctgctgt ggctgacccc aaccgccctg cggctgacca
     7801 ccaccgatat tgccacctgc cggagcgcca ccaccctgac gaccacccag catctgcatg
     7861 gtgccgccaa cgttcaccac gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc
     7921 catttacggg tacgcagctg accttcgata taaacctgag aacctttacg cagatattcg
     7981 ctcgccactt ctgccagttt gccgaacagc acaacgcggt gccattcagt ctgttctttc
     8041 atctcgccgg tcgctttatc acgccaggat tcggaagtag ccagcgtaat gttggcaact
     8101 gcgccaccat ttggcatgta gcgtacttcc gggtcctgac ccagattacc aacgagaata
     8161 accttgttta cgcctctgct ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa
     8221 gtgtaaacgc gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc
     8281 cgggaaacaa acctggccag acattgttac acaacactcc gggtaatgca ttccaatact
     8341 gtatattcat tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca
     8401 tgccaccggg caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg
     8461 ggcgcccgca cccataatct caaaaacatc aacctcgtta tcccccgcga caagctcatt
     8521 gtcgtgaccg ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc
     8581 gaagggcagc gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg
     8641 atggaaaagc cggacgtcga tcatattgag gggctttctc ctgccatctc aattgagcag
     8701 aaatcgacgt ctcataaccc gcgttctacg gtggggacaa tcaccgaaat ccacgactat
     8761 ttgcgtttgt tgttcgcccg cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg
     8821 gcggcgcaaa ccgtcagcca gatggtggat aacgtgctgt cgcagccgga aggcaagcgt
     8881 ctgatgctgc tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag
     8941 aacctggcaa gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc
     9001 gatccgccga aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc
     9061 aaggtgcgtg acgatcttac ccaacgtctt gcggagtcgt ttgaaaccgc gctggagctt
     9121 tccggtggta cagcggtagt ggcggatatg gacgacccga aagcggaaga gctgctgttc
     9181 tccgccaact tcgcctgccc aatttgcggc tacagtatgc gtgaactgga gccgcgactg
     9241 ttttcgttta acaacccggc aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa
     9301 tatttcgatc ctgaccgcgt gatccaaaac cccgagctgt cactggctgg cggtgcgatc
     9361 cgtggctggg atcgccgcaa cttctattac ttccagatgc tgaaatcgct ggcagatcac
     9421 tataagttcg acgtcgaagc gccgtggggc agcctgagcg cgaacgtgca taaagtggtg
     9481 ttgtacggtt ctggcaaaga aaacattgaa ttcaaataca tgaacgatcg tggcgatacc
     9541 tccatccgtc gtcatccgtt cgaaggcgtg ctgcacaata tggagcgccg ttataaagag
     9601 acagaatcca gtgcggtacg tgaagaatta gccaagttta tcagcaatcg cccatgcgcc
     9661 agctgcgaag ggacgcgtct gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg
     9721 ctgcctgcta tctccgacat gagcatcggt catgcgatgg aattcttcaa caatctcaaa
     9781 ctcgctggtc aacgggcgaa gattgcggaa aaaatcctta aagagatcgg cgatcgtttg
     9841 aaattcctcg ttaacgtcgg cctgaattac ctgacgcttt cccgctcggc agaaacgctt
     9901 tccggcggtg aagcccagcg tatccgtctg gcgagccaga ttggtgcggg cctggttggc
     9961 gttatgtacg tgctggacga gccatctatc ggcctgcacc agcgcgataa cgagcgcctg
    10021 ttgggtacgc ttatccatct gcgcgatctc ggtaataccg tgattgtggt ggagcacgac
    10081 gaagacgcaa ttcgcgccgc tgaccatgtg atcgacattg gcccgggcgc aggtgttcac
    10141 ggcggtgaag tggtcgcaga aggtccgctg gaagcgatta tggcggtgcc ggagtcgttg
    10201 accgggcagt acatgagcgg caaacgcaag attgaagtgc cgaagaaacg cgttccggcg
    10261 aatccggaaa aagtgctgaa gctgacaggc gcacgcggca acaacctgaa ggacgtgacg
    10321 ctgacgctgc cggtgggtct gtttacctgc atcaccgggg tttcaggttc cggtaaatcg
    10381 acgctgatta acgacacact gttcccgatt gcccaacgcc agttgaatgg ggcgaccatc
    10441 gccgaaccag caccgtatcg cgatattcag gggctggagc atttcgataa agtgatcgat
    10501 atcgaccaaa gcccaattgg tcgtactcca cgttctaacc cggcgaccta taccggcgtg
    10561 tttacgcctg tgcgcgaact gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg
    10621 ccaggacgtt tcagctttaa cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt
    10681 gtgatcaaag tagagatgca cttcctgccg gatatctacg tgccgtgcga tcagtgtaaa
    10741 ggtaaacgct ataaccgtga aacgctggaa attaagtaca aaggcaaaac catccacgaa
    10801 gtgctggata tgaccatcga agaggcgcgt gagttctttg atgccgtacc tgcactggcg
    10861 cgtaagctgc aaacgttgat ggacgttggc ctgacgtaca ttcgcctggg gcagtccgca
    10921 accacacttt ctggtggtga agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc
    10981 ggcaccgggc agacgctgta tattctcgac gagccgacca ccggtctgca cttcgccgat
    11041 attcagcaac tgctcgacgt actgcataaa ctgcgcgatc agggcaacac cattgtggtg
    11101 attgagcaca atctcgacgt gatcaaaacc gctgactgga ttgtcgacct gggaccagaa
    11161 ggcggcagtg gcggcggcga aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc
    11221 gaagcttcgc atacggcacg cttcctcaag ccgatgctgt aatcgttaag gccgctttct
    11281 gagcggcctt ttcctttcag agttgtacca gcaatttacg cttttcttcc ggtagtaaat
    11341 tcactgcctg ctgataagac gcatccacca gataatagat ttgcgaatcc ggcagcgaac
    11401 cgtcgagata cacggtgctc cagtgcgctt tattcagatg gcggctagga cgcacatcgc
    11461 tgtgctgctg acgtagcagc tccgccagct ccgggctggt tttcagcgaa acagctgggc
    11521 gattttctac ttctttcacc atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt
    11581 tccagtcgtt atgcacgctc tgttctgcgc ctggttttgc catgcaatat tgtagcaact
    11641 ccgaaatggt cattttttac tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt
    11701 ggatgcgatg ttcccccagc cagatgcctt gccaggtgcc ggtctgaata cgccctttat
    11761 gtaccggcaa tacaagcgat gttcccagca ttgaggattt gatatgagaa ggcatatcgt
    11821 ctgctccctc atagtcatgc tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac
    11881 gctccatgtc gtggcgtacg gtgggatcgc agttctcatt aagtgtcaga gaggcggagg
    11941 tatgttgcag caacagatgc agtaagccga tgttaacgcg cggcatatca gccagctgat
    12001 tcagaatttc atccgttacc agatgaaacc cacgagattt ggcgctaagc gtgagcgtct
    12061 tttgatacca catgtgctgc tccttgataa aactctctta atcagtttgc agcaagacag
    12121 cgaaaggata aaggtgtgat taaaaaaaca gcattcggga gagcgtcacg ctctcccgct
    12181 ctgtcagtat tctgaattga cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg
    12241 tttgtaggta gagttggagg cgcgcagaat gcggatacca cgagcgccga catcgcgtgc
    12301 ggcggtaata tcattatcag aatcgccata aaaaattcgg atatttttat cctgcagcca
    12361 ttgcgatttt gtattttgcc ctagtttatc gcccgcaaag atcaccggat tcatgttggt
    12421 ggcaggaata tgaaaattat ccgccagcgt ttttgaaacc gtttctgttt tcgtcggact
    12481 acgaccagtc acaaagaaga tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg
    12541 agcgacctct tttggaatgc tgaattcatc ccagccattg ttcatttttt cccagaacac
    12601 aggatttttc agataatctt cgctttctgg cgagaaggtt tttttgccgc gccagaagcc
    12661 cggactggaa aaaagtaccg tgtcatcgat atcaaacccc accgccattg gcggacgccc
    12721 tgcgaggcta ttttcaattt gtgcgaccga aacccaatga atgggtgcct gttcagcaag
    12781 cctggcaacg ttagtcccag ggttaagcgg tgaaggagat gaggccaggg caacagcgga
    12841 actgtttaga gcgaacaata agcaaacggc actgattgcc tgtgtgatct tgcgcatatt
    12901 tttccctaaa tagtcagtat gttgaaactt tttattgtga gatttgttgc aaaaaccatc
    12961 tgaccataac gccagctgcc ggggaaagga aggattatct gcggtttttg tgatgaaaag
    13021 caggatgatt atatttaacc cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt
    13081 tattactcga tgtaaggata cattaatttt acgtttatta tgatgaatcc tcggcaaatg
    13141 agcgaaattt gatagaacat ttatctgctg tacgatttaa tgaacttaat gatacagcat
    13201 tacaaaaaca atcgaagttt ataaagatga tttctgattg accagccccc taccctacaa
    13261 tggtgattaa ttttattact cgcgatggag atgatgatat gaatatatca tatgtg
//
LOCUS       DAZKGS010000124        13256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKGS010000124 DAZKGS010000000
VERSION     DAZKGS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..393)
                     /locus_tag="ACHOQY_003535"
     CDS             complement(19..393)
                     /locus_tag="ACHOQY_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679568.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHOQY_003536"
     CDS             complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHOQY_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9679569.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHOQY_003537"
     CDS             complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHOQY_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP9679570.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(794..949)
                     /gene="ydfA"
                     /locus_tag="ACHOQY_003538"
     CDS             complement(794..949)
                     /gene="ydfA"
                     /locus_tag="ACHOQY_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9679571.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1142..1549)
                     /locus_tag="ACHOQY_003539"
     CDS             complement(1142..1549)
                     /locus_tag="ACHOQY_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9679572.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            1627..1854
                     /locus_tag="ACHOQY_003540"
     CDS             1627..1854
                     /locus_tag="ACHOQY_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9679573.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            1838..2359
                     /locus_tag="ACHOQY_003541"
     CDS             1838..2359
                     /locus_tag="ACHOQY_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9679574.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            2340..3305
                     /locus_tag="ACHOQY_003542"
     CDS             2340..3305
                     /locus_tag="ACHOQY_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679575.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            3346..3765
                     /locus_tag="ACHOQY_003543"
     CDS             3346..3765
                     /locus_tag="ACHOQY_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9679576.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            3820..4917
                     /locus_tag="ACHOQY_003544"
                     /pseudo
     CDS             3820..4917
                     /locus_tag="ACHOQY_003544"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            4990..5139
                     /gene="ydfW"
                     /locus_tag="ACHOQY_003545"
     CDS             4990..5139
                     /gene="ydfW"
                     /locus_tag="ACHOQY_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFP9679577.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(5576..5908)
                     /gene="flxA"
                     /locus_tag="ACHOQY_003546"
     CDS             complement(5576..5908)
                     /gene="flxA"
                     /locus_tag="ACHOQY_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFP9679578.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(6111..6416)
                     /gene="ydfV"
                     /locus_tag="ACHOQY_003547"
     CDS             complement(6111..6416)
                     /gene="ydfV"
                     /locus_tag="ACHOQY_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFP9679579.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            6441..6680
                     /gene="relB"
                     /locus_tag="ACHOQY_003548"
     CDS             6441..6680
                     /gene="relB"
                     /locus_tag="ACHOQY_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFP9679580.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6680..6967
                     /gene="relE"
                     /locus_tag="ACHOQY_003549"
     CDS             6680..6967
                     /gene="relE"
                     /locus_tag="ACHOQY_003549"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFP9679581.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            7039..7194
                     /gene="hokD"
                     /locus_tag="ACHOQY_003550"
     CDS             7039..7194
                     /gene="hokD"
                     /locus_tag="ACHOQY_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9679582.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7411..7662
                     /gene="rem"
                     /locus_tag="ACHOQY_003551"
     CDS             7411..7662
                     /gene="rem"
                     /locus_tag="ACHOQY_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFP9679583.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            7729..8007
                     /locus_tag="ACHOQY_003552"
     CDS             7729..8007
                     /locus_tag="ACHOQY_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679584.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            8009..9058
                     /gene="ydfU"
                     /locus_tag="ACHOQY_003553"
     CDS             8009..9058
                     /gene="ydfU"
                     /locus_tag="ACHOQY_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9679585.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            9072..9824
                     /gene="ydfT"
                     /locus_tag="ACHOQY_003554"
     CDS             9072..9824
                     /gene="ydfT"
                     /locus_tag="ACHOQY_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9679586.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(10102..10191)
                     /locus_tag="ACHOQY_003555"
     CDS             complement(10102..10191)
                     /locus_tag="ACHOQY_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679587.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(10246..10458)
                     /gene="cspF"
                     /locus_tag="ACHOQY_003556"
     CDS             complement(10246..10458)
                     /gene="cspF"
                     /locus_tag="ACHOQY_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFP9679588.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            10759..10974
                     /gene="cspB"
                     /locus_tag="ACHOQY_003557"
     CDS             10759..10974
                     /gene="cspB"
                     /locus_tag="ACHOQY_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFP9679589.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            complement(11338..11508)
                     /gene="ynfU"
                     /locus_tag="ACHOQY_003558"
     CDS             complement(11338..11508)
                     /gene="ynfU"
                     /locus_tag="ACHOQY_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFP9679590.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
     gene            11728..11943
                     /gene="essD"
                     /locus_tag="ACHOQY_003559"
     CDS             11728..11943
                     /gene="essD"
                     /locus_tag="ACHOQY_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP9679591.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            11948..12259
                     /gene="ydfR"
                     /locus_tag="ACHOQY_003560"
     CDS             11948..12259
                     /gene="ydfR"
                     /locus_tag="ACHOQY_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP9679592.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            12256..12789
                     /gene="rrrQ"
                     /locus_tag="ACHOQY_003561"
     CDS             12256..12789
                     /gene="rrrQ"
                     /locus_tag="ACHOQY_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9679593.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            12786..>13256
                     /gene="rzpQ"
                     /locus_tag="ACHOQY_003562"
     CDS             12786..>13256
                     /gene="rzpQ"
                     /locus_tag="ACHOQY_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFP9679594.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYES"
BASE COUNT         3602 a         2781 c         3308 g         3565 t
ORIGIN      
        1 acctcacctg tgaataaatt aatgattaat tgatattttg ttgtttgatt caactttccc
       61 atcggatgtg tgatgcttta aatcacagga attaatactg cttgctgtaa aatgattttc
      121 aaggggagct attcgaatcc ctttcttttt cattaacaag ccaaatcctt tattaatgat
      181 gtccattaat tccagaaagt atttttcatg taaatcctgg ttatcagaga gctgcttctc
      241 ttcgtacagc ccgataaagg ctcggcgtac gttaccagat atattgtcga tggtttcttt
      301 ttctacggta ctcaggtcaa gagtcgccag ttgagagcga accacattcg ctgccatttc
      361 ctggaatggc attggtaaat ctttaaattc cattattagc ctcgttggtt agctattaac
      421 gcgggtatgt aatcattctg gcaatgctta atgccgctgc tttttccagc ctggtgatat
      481 cctgctccag agcggacaga ttttcagcct gcttagccct ggcttcattg gcccatttca
      541 ggtcctgcgc agccttaatt ttctggtgca tccactcata aagttcatca tcggtatagt
      601 ctggcgcgat gatgacgggt tctcgtttct gcatactgat tcctcgcggt gctgtttcgc
      661 ttatcagccg ttagattttg ccgaactgga aagcgcctgt ttaaactcac tgaagctgag
      721 ggcttcttcg ccttcggcaa ggccttcgaa gtattcttcg taagcctttt ccatgattgt
      781 gttgaaatcc atatcactca cctgagtttc tttccagcca gcgacgggca ccattttcgg
      841 ttttaaacgt tttgcttttg gtatacgtca tcgcggtgaa cgtgccgtcc tggttgggga
      901 acacgccaca taccagagat tcgctgttgc caagatcgat agtatccatg ctgacctcat
      961 ttccccttaa cgccggggta gcggaactgt ttgctgagaa caccgtgcgg tgtcttgatg
     1021 gaggtaattt agttttctca ttatttttcg tcaaggtttt ttgatgagaa aactcaagtg
     1081 ttggcgcaag ataaagccaa tacattgaaa tgtaaggctt taaaattttg tgaagggatg
     1141 attattgatg cttgttgcgt ttgcgagcct ctaatagctc ggtgaacagg cgattaaaat
     1201 tctcaacgcg ggcgcggagt tcgctgagtt gtgcttgttg ctctgattta ggaagtgcgc
     1261 gatacaaacg cagcatctct aactcatctt ccgataagtc taaggcacta tcgagtgaaa
     1321 cagggggagc tggtgttttg tcctcgtcgc caaacagtat ccaagttggt gaacattgca
     1381 atacctcggc gaggcgatgc aaattttgcc cgcgcggggc tgtatggtca ctttcccata
     1441 atgaaattga tgagccagat acgccagcag ctttgcttaa accgttttga cttaagccta
     1501 cctgcttacg tctttctcta atgcgttgac ctaaagtttt ctcgttcata tttagatatc
     1561 ttaataaccc ttgacttgag attccttgag tgattagcat tgagaaaact caatattgga
     1621 ggtgcgatgt ttaaatcaga cgtaattaat ttttacggta cgaaagccaa agtagcgaaa
     1681 gctgctggcg ttgacccatc tgctgtttct caatggcaag agctggttcc tgaaggtcgc
     1741 gcgatgcgtc tacaggaggc atctggcggc gagcttctgt atgatcccaa ggtttatgac
     1801 gaatatcgta agacgaagcg ggcggggcgg ttgaacaatg aaaatcactc ctgaacaggc
     1861 tcgtgaggct ctggatgcct ggatatgtcg accaggaatg acacaggagc aggcgacgat
     1921 attaatcact gaagcattct gggctttgaa agagcgcccg aacatcgatg ttcagcgtgt
     1981 cacagatgaa ggtggcgcgg ttgatcagcg agcacttggc gttaatcgag tgaagatatt
     2041 cgaacgctgg aaggctatcg acaccaggga taagcgtgaa aagttcacgg cgctagtgcc
     2101 tgcaattatg gaggctattc ggattaatga tttcaggttg tatcgtgaaa ttagtgacgg
     2161 aaaaagcatc acgtacatga tcgccgggtt aaacaaagaa tatggcgatg tggtggagtc
     2221 cggactgctt tttgctgatc ctgccgtagt ggatcgtgaa actgacgaac ttatagaaaa
     2281 agcaattgct ttcaaacttg cgtatcgaca gcaataccaa caaaaagctg gatggaatta
     2341 tgagccttct ttttgctgaa cgcccactgg ttataaacac acagcttgcg atgaagattg
     2401 gcttaaatga agccattgtg ttgcagcagt tgcattactg gttgagagat accaattccg
     2461 gcatggaatg tgatggtgtt cgctggattt acaacacaac ggaacaatgg ctggaacagt
     2521 tcccattctg gtcagagtca acgttaaagc gcgcgtttgc aagtctgaaa acgctggggc
     2581 ttttgcgttg tgaaaagctc aataaatcaa agcgcgatat gaccaatttc tacacgatca
     2641 actacgggag cgagctttta gatggtggca aattgagcga atccatcggt ttaaaatgcg
     2701 ccgctccatc aggtcaaaat gacacgatgg aagaggtcaa aatgaaacgc tccattggtt
     2761 caaaacgacc caatgtcatc gggtcaaaat ggcctgatga tcttacagag aatacaacag
     2821 agattactac agagaataaa aacacttttc gtccggaagc ttcgcaaccg gaccagcaga
     2881 ccgctgaaca ggatttttta acccggaacc ccgacgcggt tgtgtttagt gcgaaaaaac
     2941 gccagtgggg tagcagggag gatttggcgt gtgcgcagtg gatttggggg cggatcgtga
     3001 acctttacga acaggctgcc agcgacgatg gagagatcat gcgaccaaaa gagcctaact
     3061 ggacagcctg ggccaatgac gtgcgcacaa tgcggatgct ggatggcaga agccacagac
     3121 aaatttgcga aatgtttggt cgggtacagc gagatcaatt ctgggtaaaa aacatcatga
     3181 gcccgtcaaa actccgcgaa aaatgggacg aactggtcat ccgcctggga cgttcacctg
     3241 tacagcgttg tgtgaatcat atttctgaac cggatacaga aattccgcct ggtttcaggg
     3301 gataagtgtt gattttaggg tataaagtaa tgttcaggag gacttgtggc aaaagttttt
     3361 acacaagaag agcgggaaaa aattaaaggg caggtggtgg aactcgtacg ccagagtggg
     3421 cgcgagacgt tacgacaact ggaagctaaa actggggcaa caagatatct gatgagcgtt
     3481 ctggccagag agctggttgc cagtggcgat gtatacagct ctggttacgg gttattcccg
     3541 tctgaacagg cgcgtaagga ctggcaaaac gcccgcaaaa aactctcgag ggcaaaggtg
     3601 aagaaacctg ctgtggttga tccggacctt atctggtcat tacctgacgg agaaatacgt
     3661 cgctacgaca gtcgtctaaa cataatctgt cgcgagtgcc ggaaggcaag gagaagcagg
     3721 ctattgttaa caccaagctg taatgtcccc tttgaaccat tctaaaatgt ccccagacaa
     3781 ttctctgggg gatttttcat gatcaaagag actgttacga tgagtcataa ggaactccac
     3841 cgacttcaga ttattcagga gtcaattaat cgacatatta ctcaggaaca agctgcggca
     3901 cgcattggca tttctattcg gcaggttaaa cgtctggtgc aacggtatag aaatgaaggg
     3961 ccttctggtc tggtttccca ccgacgtgga aagcgtccta ataattcctt ttctactgaa
     4021 ttcagagcaa cagtaatttc actcctcaaa ggccgttacg ctgattttgg acctacgttt
     4081 gcgtgcgaaa aattgcgcga gatacacggt ttatctttat ccgttgaaac tctcagaaag
     4141 tggatgatag aagaggggtt atggcgtgaa cgccgtcgta aaattgcccg tatatatcaa
     4201 cgccgcatgc gacgaccatc ttacggtgaa ctgatccaga ttgatggctc acctcatgac
     4261 tggtttgaaa atcgaggccc cagatgtaca ctgatcgttt tcattgatga tgccaccagt
     4321 gcgttgatgg cgttgcgttt tgtgcctgct gaaacaaccc gggcttacat ggaaaccctc
     4381 cggggttacc ttaatgatca tggcgtaccg ctcgctctct actctgatag acacagtata
     4441 ttcagggtaa ataacccaga gcgggaaggt gagctgaccc agttcactcg tgcgataaag
     4501 acactgggca tcgagccaat ccatgccaac agcccgcagg caaaagggcg ggtagagcgc
     4561 gccaatcaga cactacagga caggctggtc aaagaaatgc ggcttcagaa tatcagtgat
     4621 attgaaacag caaatgcatg gttgccgacc tttattgaag cctataacaa ccggttcgct
     4681 acgtcgcctc gtactactga taatgctcat cttgatgtgc accattctga agaggaactg
     4741 ggttatatct tcagcctaca ggcgaagcgc gttctgtcta aaaatctcac tttccagtac
     4801 aaaagcagtg cgtttcaggt acgcagtgag ggccggggat atcgacttag gcattcggtt
     4861 gttactgtat gtgagaactt tgacggtgaa attaacgttc tgtatgacgg gaaagcgctg
     4921 ggctgggaaa agtatgttga tggcccggag cctataccac tggatgatga aaagagtgtc
     4981 catgaacgag tggataatgc ccgtattgat ttacgctcaa aatactatgt taaatctaaa
     5041 gctgaccatc cctggcttac gcgccgaacg caaagtcatc agcaagttaa gcccccgaag
     5101 ttacctaaaa agaagcctga tcccgataaa atagattgaa accaagatcg attcggttga
     5161 gtgcatatcc attcataggg tagattctta agtcgcgttt ctgatgttca ttttcgggtg
     5221 gtttgttact tgttttaccg gggatatgcc agaaacgcgc tgagtcagtc tgggcggtgc
     5281 gcgtaatgag gcgttatggt aaatagccta tgctaatgtc cgctaagagc aagaagcgga
     5341 agttggcagt tttgtggact gtccccacaa aagtgactac agaaatagtt gcaattcata
     5401 attgatcatg ggttgtcagt taaactcgtg gcgatttaaa tagactaatt gggagtgcgt
     5461 ccattactta tatcttgtaa tgttaactat cagaaatgat acaaagataa tatgtcttta
     5521 aagaaaaggc tgatggcgaa aagtggcccg atgagggcca caatacggct gtcacttaga
     5581 cgtaaatatc aatggtgcca gcggtatttg tatcgtcttt tttctcttct tttttatcag
     5641 gctgaactgt cgcgtcttta ttctttttct ctgcctgctg ccttaacaac tgctccagtt
     5701 gagccctgag gctttcaatt tgcttctgta ccaatgcagc catttctttt ttctgctgtg
     5761 tcgtcatccc ctcttccgat gagattttcc caagcttttc agtcagcacc tgaatttgtc
     5821 ttgtgatttt ggctatttct gatgttcctt ccggggcgga gttgtttgaa ataacggttg
     5881 aggtatttcc ctgaattgtg acagacatag atttctcctt ttaaaaaagc actatcggca
     5941 tgcacaaaaa aatctttaat cgtatttctt gtgtcattaa ttgtttgatg ttcagattgt
     6001 tttcctcgcg ggctggcgcg cctcagaaag taaagcttgt tgacaggggt aaacgttcgg
     6061 caataatttt ctgccgcatg cgggtgttgc ataaaacgtg ctacgttcct ttatcgacag
     6121 gtcaggtcac cgctcacccg ccgacgagaa agcaacactg acatgctaaa gcaaaaaata
     6181 gatgaataag ttgagttgtg catatgtagc ctgaccgtca caaagtatat ggtgtctgta
     6241 ccagtaagat gatggccgga ctctttaaaa acgagctgac ctgcacaata caggatggac
     6301 ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca ccgttcttac gactactttc
     6361 tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg atacttgtaa tgacatttgt
     6421 aattacaaga ggtgtaagac atgggtagca ttaacctgcg tattgacgat gaacttaaag
     6481 cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc ttctgaagcg cttcgtctca
     6541 tgctcgagta tatcgctgac aatgaacgct tgccgttcaa acagacactc ctgagtgatg
     6601 aagatgctga acttgtggag atagtgaaag aacggcttcg taatcctaag ccagtacgtg
     6661 tgacgctgga tgaactctga tggcgtattt tctggatttt gacgagcggg cactaaagga
     6721 atggcgaaag ctgggctcga cggtacgtga acagttgaaa aagaagctgg ttgaagtact
     6781 tgagtcaccc cggattgaag caaacaagct ccgtggtatg cctgattgtt acaagattaa
     6841 gctccggtct tcaggctatc gccttgtata ccaggttata gacgagaaag ttgtcgtttt
     6901 cgtgatttct gttgggaaaa gagaacgctc ggaagtatat agcgaggcgg tcaaacgcat
     6961 tctctgaacc aaagcatgac atctctgttt cgcaccgaag gtgacacttc tgctttgcgt
     7021 tgacaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc
     7081 tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc
     7141 cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc
     7201 cggcggggga gaaatccctc gccacctctg atgtggcagg catcctcaac gcacccgcac
     7261 ttaacccgct tcggcgggtt tttgttttta ttttcaacgc gtttgaagtt ctggacggtg
     7321 ccggaataga atcaaaaata cttaagtagc gcgcagggat aagagggatg gtcccttaaa
     7381 ggggagagct aattatccgg aaggattctg atgatgaaca tcgaagaact gcgtaaaatt
     7441 ttttgtgaag atggcctcta tgctgtgtgc gttgaaaatg gaaatcttgt tagtcattac
     7501 cgcattatgt gtttgcgaaa gaatggggct gcgttaatta attttgtgga tgctcgggtc
     7561 acggacggat ttatcttgcg cgaaggtgag tttgtcactt cattacaggc attgaaagag
     7621 atcggaataa aagctggctt ttctgctttt tcaggagaat aaactcatct acaatcttgc
     7681 gcggggctga actcccgctg agtaacaccg tgccaccgga gaaaaccgat ggcacgcaac
     7741 gcaaaatatt acaattctga taattcgccc gttcttgcct gcacgcacgg gcggtattct
     7801 cacgcattca agtctgaatg gttccagcac cctccatgca ctgcagaaca ggccgaatgg
     7861 ctgattcatt cttaccgcag gcgcgggttc gaggttaaga aagctctcag tctcgactat
     7921 cggcactgga taatctctgt caggctgcct tattccgaac gcccaccacg tgcgtcccgc
     7981 actttccagc aacggatctg gaggtaacgt gcgggtatta cttagacctg ttctggtgcc
     8041 tgagcttggg ctggtggtcc ttaagccggg ccgtgaatcc atacagatat ttcataatcc
     8101 tcgagtgctg gtggaaccgg aaccaaaaag catgcgtaat ctgccatccg gagtcgttcc
     8161 tgccgttcgc cagccgctgg cggaagacaa aacattgctg ccgtttttta gtaacgaacg
     8221 ggtgattcgt gctgctggcg gcgttggcgc attgtccgac tggctattac gtcatgttac
     8281 atcctgccag tggcctaatg gcgattacca tcacactgaa acagtcattc accgttatgg
     8341 taccggcgca atggtgttgt gctggcactg cgacaaccaa ctgcgtgacc agacatcgga
     8401 atcactggag ctgcttgctc aacaaaatct gacagcatgg gtgattgacg tcatccgtca
     8461 cgcaataagc ggtacgcagg agcgggaatt atctttggct gaattatcct ggtgggcggt
     8521 ctgcaatcag gtggtggatg cactacctga ggctgtatcg cgtcgttcgc tgggattacc
     8581 agcggaaaaa atctgctcgg tgtaccgcga aagcgacatc gtaccgggag agcagaccgc
     8641 caccagcata ttgaaacaac gcacaaaaaa tcttgcaccg ttgccttacg cccaccagca
     8701 acaaaaatca ccacaggaaa agacggtggt aagcatcacc gttgatccag agtctccgga
     8761 atctttcatg aagctgccta aacgtcgccg ctgggttaag gagaaataca cacgttgggt
     8821 taagacacag ccgtgtgctt gctgcggtat gccagccgac gatccgcatc atctgattgg
     8881 tcacgggcag ggcggaatgg gaacaaaagc acatgatctc tttgtgttgc ctttgtgcag
     8941 aaagcatcac aacgagctgc atacggatac agtggcattt gaagataagt atggctccca
     9001 actggagctg atatttcgtt ttatcgatcg cgcgctggca attggcgtac tggcgtaagt
     9061 ggagaacgag catgaacctt gaagccttac caaaatatta ctccccaaaa tctccaaaat
     9121 tgagcgatga cgctccagcg acaggcaccg gttgtttaac aattacggat gtaatggcag
     9181 cgcaggggat ggtgcagtcg aaagcaccac ttgggttggc cttatttctg gcaaaagttg
     9241 gtgttcagga ccctcagttt gcgattgaag gcctgctaaa ttacgcgatg gcactggata
     9301 acccgacatt gaacaaattg agtgaagaaa tccggttaca gattattcct tacctcgtga
     9361 attttgcctt tgctgattac tccaggtctg cggcaagtaa ggctcgctgt gagcattgtt
     9421 caggtacggg attttataat gtattgcgcg aagtggtgaa acactaccga cgcggggaat
     9481 ctgtaatcaa ggaagaatgg gtgaaggaac tatgtcagca ttgccatggt aagggtgaag
     9541 tcagcacagc gtgcagaggg tgtaagggta aagggattgt tctggatgaa aaaagaaccc
     9601 ggtttcatgg cgtaccggta tataagattt gtgggcgttg taatggaaac cggtttagtc
     9661 gtttaccgac cacgctggca cgacgtcatg tccagaagct ggtaccagac ctgaccgatt
     9721 atcagtggta taaggggtat gcggacgtca ttggtaaact ggtaacaaag tgctggcagg
     9781 aagaagcata cgcggaagcg caattgagga aggtgacgag ataaatgatt tttgctgaag
     9841 atggcgacat gatgtttgca tttttcaaaa aatatggata aaattttttc aacgatgggc
     9901 tttgtatacc cgacgttaag aaaaagtaga aaacccgctg atgagcgggt tttgtgcttt
     9961 aaatggggca atggtaatgt tgaatctcat cccgggactc atgtctgtta acttattatt
    10021 tagctggtga cttggttatt tgcctgatgt ttaaaatgtt ttcttccagt acaatgtccc
    10081 taaacacaat gagtctgctt attatattat tagcagagct attacggcca aagtacagca
    10141 taagctttta aagccaatca accagtcatc aagacagacg gggttattca taaaaactct
    10201 ccatgtgtga tccgatgggg cctgaaatta aagctttaat atagctcatg aaaggtaaac
    10261 attggcagct gaagggccac gcagaccatt tatccggcaa aattccacgc gtaatccggt
    10321 ggtaatttct tctgcatcgc ggagattgag cgctgaaaca tgaagctgga catcgatacg
    10381 accatcggat ggggtgataa gacccttgcc gcttttgccg tcaaaggttt tgacaattcc
    10441 tgtcatttta cgggacaaaa aaattcctta atactgataa cttggcgcac tatacacacg
    10501 ttcctgaaga aagctatagt tttttgatgg ggttgaagat ggctggatgt ctaaaataaa
    10561 cattgcttca tatgttcaac tatgcgttaa tgattgcgtc ggtttgaaga acagacgata
    10621 tacgaagtag tttactaaag cagttctcat ttcaggtgtt attcacttat tccttctttg
    10681 agtctctcca attaagtacg aagtcgtttc tgttatgcaa accatttatg ccgaaaggct
    10741 caagttaagg aatgtagaat gtcaaataaa atgactggtt tagtaaaatg gtttaacgct
    10801 gataaaggtt tcggctttat ttctcctgtt gatggtagta aagatgtgtt tgtgcatttt
    10861 tctgcgattc agaatgataa ttatcgaacc ttatttgaag gtcaaaaggt taccttctct
    10921 atagagagag gtgctaaagg tcctgcagca gcaaatgtca tcattactga ttaaaattca
    10981 tcgctcgtct gtatacgata acgaagaagg ctgatgcctg agtagagata cggacagagt
    11041 agtgaatatt ggatctcttt aataaaaagt aaggaggtcc aatacatgaa acaatggcta
    11101 gcatatttgg caaaatctta atcaggaaaa gtatgctaac cattgtggtg aagtgcaggt
    11161 ttgctgcatg aatagtttca cagcagaagc taactgctgg catcgcaaaa caaagtgcgt
    11221 aagtggatga ctcccacaaa aagcaccaca atctcaaacc cgctcaggcg ggttttttat
    11281 tatctgcttt aaatatatta ttaaaatata aaaaatactt gttactaata aaatcaatca
    11341 ggctacagct ttaagatttg tctggaatac tttgttgcaa tgagggcaga tcaaaagggc
    11401 acctttttgt actcttgaaa aactgtgttc tgactcttgg gtgcagtttg ggcaggaaca
    11461 tttaacgaga taattacggc gtgattttga gtctttacgt tctgacatag gcttttcctg
    11521 tataatggcc gtatacagta cactaaatat gaaaacattt ctcgtattat tattttatat
    11581 atgactttct ttcaaaataa ttactcacat ttttaatgtg tatgtttctt tagcgccgtt
    11641 gagaacaacg tgtgctgtca aaactacccc gtagactccg atctttttca aacatattgc
    11701 accatccgtg tacatcgggg tgaggatatg aaatcaatgg ataagttaac aacaggtgtt
    11761 gcctatggca catcggcggg taatgctggt ttctgggcat tacagttact cgataaagta
    11821 actccgtcac agtgggctgc aatcggtgtg ctgggtagcc tggtttttgg cctgctgacg
    11881 tatctgacaa atctttattt caagattaaa gaagacaggc gtaaggctgc gagaggagag
    11941 taatccaatg actcaagact atgaactggt tgtgaaagga gtccgtaatt ttgagaataa
    12001 agttacggta actgtagcct tacaggacaa ggaacgcttt gacggtgaaa tttttgacct
    12061 ggatgtcgcc atggaccgtg ttgaaggagc tgcgctggag ttttatgagg cagcagccag
    12121 aaggagcgtc cggcaagtct tcctggaagt agcagaaaaa ttgtcagaaa aagttgagtc
    12181 ttatctgcag catcagtact cctttaagat tgaaaatcct gccaataagc acgagcgtcc
    12241 tcatcataaa tatatatgaa cacaaaaatc agatacggcc tgtcggctgc cgttctggcg
    12301 ctgattggtg ctggcgcatc tgctcctcag atacttgacc agtttctgga cgaaaaagaa
    12361 ggtaaccaca caatggcata ccgcgatggt tctggcatat ggaccatctg tcggggtgcc
    12421 acagtggtgg atggaaaaac cgtttttccc aatatgaaac tgtcgaagga aaaatgcgac
    12481 caggtcaacg ccattgagcg tgataaggcg ctggcatggg tggagcgcaa tattaaagta
    12541 ccactgaccg aaccacaaaa agcgggtatc gcgtcatttt gtccctataa cattggcccc
    12601 ggtaagtgtt tcccgtcgac gttttataag cggctgaatg ctggtgatcg taaaggtgca
    12661 tgcgaagcga ttcgctggtg gattaaggat ggcggacgcg attgccgcat tcgttcaaat
    12721 aactgttacg gtcaggttat tcgtcgtgac caggagagcg cattaacctg ctgggggata
    12781 gaacagtgaa tcagatattc atggtgattt ttctcgtgtt gtcaggattt atcgtcggaa
    12841 atgtctggag cgacagagga tggcaaaaaa aatgggcgga acgtgatgct gccgcattat
    12901 cacaagaggt aaatgctcaa tttgctgctc gaataattga acaggggcga actatagccc
    12961 gtgatgaggc tgttaaagat gcacaacaga aatctgctga aatttctgcc agggctgctt
    13021 atctgtctga tagtgttaac cagttgcgtg ccgaagcaaa aaaatatgcc atacgccttg
    13081 acgcagcgaa gcataccgca gatcttgccg ctgccgtcag aggcaaaaca accaaaaccg
    13141 ccgaaggaat gctcaccaac atgctcggag atattgcagc agaagctcag ctttatgctg
    13201 aaattgctga cgaacgctac atcgcaggag tgacttgtca acagatctat gaatct
//
LOCUS       DAZKGS010000125        13174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKGS010000125 DAZKGS010000000
VERSION     DAZKGS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..216)
                     /locus_tag="ACHOQY_003563"
     CDS             complement(<1..216)
                     /locus_tag="ACHOQY_003563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679595.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHOQY_003564"
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHOQY_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP9679596.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAILLSGGLYWIALQLI"
     gene            complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHOQY_003565"
     CDS             complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHOQY_003565"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9679597.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            2567..3280
                     /gene="yghS"
                     /locus_tag="ACHOQY_003566"
     CDS             2567..3280
                     /gene="yghS"
                     /locus_tag="ACHOQY_003566"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9679598.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3312..4070
                     /locus_tag="ACHOQY_003567"
     CDS             3312..4070
                     /locus_tag="ACHOQY_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679599.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4116..5465
                     /locus_tag="ACHOQY_003568"
     CDS             4116..5465
                     /locus_tag="ACHOQY_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP9679600.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5465..6289
                     /locus_tag="ACHOQY_003569"
     CDS             5465..6289
                     /locus_tag="ACHOQY_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679601.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6301..6861
                     /locus_tag="ACHOQY_003570"
     CDS             6301..6861
                     /locus_tag="ACHOQY_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9679602.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6892..7962
                     /gene="lptF"
                     /locus_tag="ACHOQY_003571"
     CDS             6892..7962
                     /gene="lptF"
                     /locus_tag="ACHOQY_003571"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9679603.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7959..9038
                     /gene="lptG"
                     /locus_tag="ACHOQY_003572"
     CDS             7959..9038
                     /gene="lptG"
                     /locus_tag="ACHOQY_003572"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9679604.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9074..10246)
                     /locus_tag="ACHOQY_003573"
     CDS             complement(9074..10246)
                     /locus_tag="ACHOQY_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9679605.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10246..10494)
                     /locus_tag="ACHOQY_003574"
     CDS             complement(10246..10494)
                     /locus_tag="ACHOQY_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9679606.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10526..11440)
                     /locus_tag="ACHOQY_003575"
     CDS             complement(10526..11440)
                     /locus_tag="ACHOQY_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9679607.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11437..13125)
                     /locus_tag="ACHOQY_003576"
     CDS             complement(11437..13125)
                     /locus_tag="ACHOQY_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP9679608.1"
                     /translation="MRYADFPTLVDALDYAARSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3148 a         3257 c         3426 g         3343 t
ORIGIN      
        1 tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatacacagg gcttttgtta ttgcttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcaac
      301 cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg aatatggggt
      421 caacccacgg tctggcgatg gtcttttcca tgctgctggc ggcgattata tggaacctgg
      481 ggacgtggtt cttcggttta ccggcctcca gttcgcacac cttgattggc gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggtggtct gattttcctg ctgcgccgtt tctggagcgg gacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg
      841 ggcagaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac tcgcgatgcc gtctccaact
      961 tcgaacacta tctgcaacag catcctgaac tgccgcagaa gttgattgcg atggaacctc
     1021 cattgcctgc ggcatcgact gatggcgcgc aagtaacaga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctcagata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggtctt gccagttata ttgggatgcc cgtctccaca acacacgtcc
     1501 tctcgtctgc agttgcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggcg tgggtattta ctttaccggc ggcgattttg ctttctggtg
     1621 ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcctggcga
     1681 tcgggcattt ccatttttgg ctagtgataa ccacgcgcgg tcataaaatc cataatcgct
     1741 ttttctgcat caaccaacac ctgttccagt ggctgattgg catcgatatc aaccagttgt
     1801 gcaccaccaa aggttaactg tggcgttatg gctatctttc ttgccagcga ctcccttttg
     1861 tggtcaggct tacgcgcgca ggcaacttca aggtcaacat tgagtttgat gaccagatca
     1921 ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag ccatgagaca
     1981 aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc aggaatttgg
     2041 tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc aagcatatga
     2101 cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactggtcct
     2161 ggcaattttt ttgctgtttt cacctgattt cgttcaattg ttttatgtaa ggattttccc
     2221 atcaacggta attttgtcac tgcacgaccg acatttccgg cttgttttcc taaatgaact
     2281 ctttcggcag caccatattt ttcgacaacg gtaataagat gttcacacac cgttgacttg
     2341 cctgaaccat cgctaccaat aacggcaatt aatggaggtg ttattgactg catgaatata
     2401 tccttaaatt taaatatcca tccaaaatat ttatttggtt aatatgtttt tatgaaagcg
     2461 taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg atattctgag
     2521 cacggaccac gtaatattca atacattatt tactgccggg tttttcgtga atcaacgtaa
     2581 tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat gtgatggttc
     2641 aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa tgccaacaga
     2701 acgcatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac agctccctgt
     2761 tattggcgca ccttttgggc gttatctgcg aagtaaagcg gcacatgtgc acgaaaagcc
     2821 ctcaacaccg cctggcaata ttactgcgct ggttatctat ctgctttcct gctggcgggc
     2881 gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca tcaccgaccg
     2941 ctacccgcag gttgaagtgc cgggttttcg ctttgatggc ccgcaattgg caaaaaccac
     3001 gggcggtaac ggttggataa aaatgttaag gcagcgcgag ctgaagctgt accaatggat
     3061 ggcatcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa ccgcgtttgc
     3121 gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact
     3181 gacattcaat ggcgcaaaga tccttgaact ggatgggcga caccccgccg atgaaattct
     3241 gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat ggatagggga
     3301 tgatttaacg aatggatgca ctacaaactc aaactgttaa tagtacaacc gcgccgcagc
     3361 ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt aaatccacac
     3421 tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgg cgctatctgg
     3481 ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc
     3541 tggaacggcg actggcggcc aaatcctcga aaacccaaag catgaaaacc aaatctccgg
     3601 cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg aatctacgca
     3661 aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg
     3721 aaatttcggg cttttattat gatggaccgg ggattggcgt cgaacgtgcg accgggaaaa
     3781 tcagcatgtt tctggcgcag cgcgaacggc gtttatacca acaaatggcg caatatcgcc
     3841 cggaattaat tattcgcctg ggcattgata ttgagactgc catctcccgc aagcctgacc
     3901 atgactatgc cgagctgcag gacaaaatcg gcgtcatgtc gacgattggc tataacggca
     3961 caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa caggcacaga
     4021 aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa ggctggcggg
     4081 atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac atcaaacatt
     4141 ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta ggctccagta
     4201 atgtcgtgag cgccttgctg ggtctgttgg cgctctcgtg tgccggtaaa gggatgacgc
     4261 ccgccatgtt tggcgtactg gtgattgtgc aatcgtacgc caagtcgatc agcgatttta
     4321 ttaagtttca gacatggcaa ctggtggttc agtacggaac gccagccttg accaacaata
     4381 atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc gtcagcggcg
     4441 cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca ttaggtctgg
     4501 atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca atggcttcct
     4561 ccacgccgac cggcattctg cgtgcggtag atcgcttcga tttaattgct gtacagcagg
     4621 cgacgaaacc ttttctgcgc gcagcgggga gcgtcgtagc ctggtatttt gactttggtt
     4681 ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc atgtactggt
     4741 ggtttgcagc gcgcgaatta cgccgcagaa atatccataa tgccttcaaa ttgaatctgt
     4801 ttgagtctgc ccgacacatc aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc
     4861 actccatctg gtcggcgcgt aactcgtgca gcacagtgct ggtggggatc gtgttaggac
     4921 ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc ggaacgccag
     4981 cgggtttgct gggtaaaagt ttttacccag aggtgatgcg tttagatccg cgcaccacca
     5041 gaccgtggtt gttaggtgtg aagtccggtt tactggcggg cggaatcggt attctggtgg
     5101 cgctcgcggt gtttatcgtc ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc
     5161 tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatttcg atgctgggct
     5221 tccctcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa
     5281 ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc
     5341 tgggagcagc gtttgcttac ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc
     5401 caaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc ggagagaaat
     5461 cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg ttgcgattga
     5521 agaagatgat gtcgttgatg cacaggttag tttgccgccg actattgcac taagttgtac
     5581 caccgaaatc attcatgata attacgcgtt atgcctgcaa ttctggctca atggcgttga
     5641 tcgtcaggaa ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc tgactgctga
     5701 tgagcgcaaa caatttaaat acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg
     5761 gctgtattta aagaagcatc aggccggatt tttattcggc aaaacgaccg tttttctggg
     5821 gcgttttcag gacggctttc gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct
     5881 gttacggatt tatttaagct ctccggtctg gtcgttggtg aactattcat tacgccatag
     5941 ccagttagaa agcgtaagca gttttattgc ctatcggcaa aagcaaatgc ataccctgaa
     6001 agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc gaaaaatcat
     6061 cagccaacag gtttcttatt acgacacctt aagatcgctt gatcctgaaa ataaagaggc
     6121 gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggagata aacacgacga
     6181 catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccgttgg cgctggatag
     6241 cgatattcgt cagcgcttag agctgctgat ttcgcgtttt ccgctatagc cggagcaatg
     6301 atgtcatctc gtttgattat tgcgttgatt atcatgctct tagccccggg tgtgcaggca
     6361 cacaatttcg tcactggcaa aacggttacc cctgtttata tccaggaagg cggcgagctg
     6421 cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca attagcagga
     6481 aaagtccgca ttattcagta tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg
     6541 ttaattaaag cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc caccaccatt
     6601 gtgaacaccg atgatgccat ctttggtact ggctattttg ttgttggcaa aatcgaaaaa
     6661 aataaacgcc gctatccgtg ggcgcaattc attattgacg gcaatggaca gggacgcgtt
     6721 gcctggcgct tgccggagca gagttcaacc attctggtgc tgaataaagc cgggcaaatt
     6781 cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat tgcgctggcg
     6841 cagaaactga tcaatgaata aaaaattcaa aggattgagg gcgttagtga gatgaagctg
     6901 gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt cggttttctg
     6961 atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc aaatgggaca
     7021 ttagctcttg atgttgtgct ggatatcgtt ttctacaaag tgctgattgc actggagatg
     7081 ttgttacctg ttggtctgta tgtgtcagtt ggcgtgacgc tagggcagat gtataccgac
     7141 tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt
     7201 ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta tggtcgtccg
     7261 tgggcttata cgcagattta tcaactggag cagcagtcgc agtcggagct ggatgttcgc
     7321 cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct ttcgcagacg
     7381 gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac tgccaatcga
     7441 acccgcattt tccgcgcccg ttcggttgat gtggttgacc catcacccga gaaaccgacc
     7501 gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa
     7561 cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc taacgtcaaa
     7621 cgcaaagcaa aatcggtcac ggagctggcg cgctccgcct ttcctgccga tcatgccgaa
     7681 ctgcaatggc gacaaagccg tggcctgaca gcattgttga tggcgctgct ggccatttca
     7741 ttaagtcggg taaaaccgcg gcaagggcga ttttcaacat tattgccact gacgttgctg
     7801 tttattgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa cggcgcgatt
     7861 cccctcattc ctggtttgtg gttagtaccc ggactcatgc taatgggcct gctgatgctg
     7921 gtcgcacgcg acttctcttt gctgcagaaa ttttcccgat gaatgttttc agtcgctatt
     7981 taatccgtca tctctttctc ggttttgccg ccgccgcagg gctattgctg ccgcttttta
     8041 ccaccttcaa cctgattaac gaactggatg atgtcagccc gggcggttat cgctggactc
     8101 aggcggtgct ggtggtgcta atgaccttac cgcgcacact ggtcgaactt tcgccgttta
     8161 tcgcgttatt gggtggtatt gttggactgg ggcagttatc gaaaaacagt gagcttaccg
     8221 ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggtgctg gttgccggga
     8281 tattgtggac tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac
     8341 aggcgttgca aatcaaatcg accgccaccg cgttggggga ggacgatgac attaccggca
     8401 atatgctgtg ggccaggcgt ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg
     8461 gccagccggt gggcgtggag atatttcatt atcgcgacga tctctcgctt gaatcctaca
     8521 tttatgcacg cagtgccacc attaaagacg acaaaacgtg ggtcctgcat ggtgtgaatc
     8581 ataaaaaatg gctgaatgga aaagaaacgc tggaaacatt agataatctt gcctggcaat
     8641 cggccttcac cagtatgaat cttgaagagt tatcaatgcc ggggaatact ttttctgtcc
     8701 gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc agcgaatacc
     8761 gccttgcact gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg attttgctgg
     8821 ctgtgccgtt tacctttact gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag
     8881 gcgtcatcgt gggcttactc acctggatca gctatcaaat catggttaat ctgggattgc
     8941 tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg
     9001 cgttgagcct ggtgtattgg tatgacagac aacattaaac ccgccgtggc gcaaacacca
     9061 cggcgggctc acattactgc ttcagagtag caaatgcctg aatgatggca tcgatttgct
     9121 cacgactgtg ggcagcattt acgctacagc gcagcagcgt gatgcctgca ggcgcggcag
     9181 gcggcaggat aagattcaca tacacgccca gcgagataag ctcgcgccag atgcgtaaac
     9241 cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcat
     9301 aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagc ttttgccgca
     9361 gctctggttg tgtggcaata gttcttaacg acgagcgcac ggtcgcaatg caggacggag
     9421 aaggagaggc ggtaaaaata tacgggcggc tgctgtagcg cagcacttcc atcgcttccg
     9481 aaccgacagc aaaaccgcca atcgaggcca gacttttact gaaagtacca acgataatat
     9541 ccacatcatc ttcaacgccg acagcttcag ccagtccgcg ccccgttgcg cctaacacgc
     9601 caaacgaatg cgcttcatcg acaatcaaat aaccaccgag acgacgtttg atatcaacaa
     9661 tttccgccag cggcgcaacg tcaccgagca tgctgtagat gccctcaacg atgataatcg
     9721 cctctttcgc ccgttcaccg aggcgaacca tgcgccgttc cagatcttta gcatcgttat
     9781 ggcggaaacg aataatttcc gcgccaccga gagaacaggc gtcataaata ctggcgtggc
     9841 tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgcgctgatg acgcctaaat
     9901 tagcggtata accggttgaa aaaacgatag cggtagggcg attgaaaaac tcggcaatct
     9961 ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc catacgagaa ccggtggtgc
    10021 cggtgccttg ggccgctaac gctgcctgac cttcgctgat ggcatcatga ttaaaggtta
    10081 aaccgagata attattggtc ccggccagaa tcactttttt attgccgatc cggccttcgg
    10141 tggcggaata cacttcatca atacaggtgc caaacggtgt taacccgttg tcactaaatt
    10201 gcagtcgctc gccagcaagg cgtgcatatt tatcgtatag ccccattatt tattctccag
    10261 ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca gaatattaat
    10321 aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca ctttgatcga
    10381 ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac cgttttcgac
    10441 caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta ccatagaaac
    10501 gttaacacac ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc
    10561 taatcccggg aaaccaatta atatcttcag aaatacggtt attacttgcc gaccagtcgg
    10621 cgtgggttaa ttcacgaatt ttcgagcggg tcagcatagg ttctttacct gccaggcgac
    10681 tcaacgcggt actgatatcc gcaaggcagg tgagtaccgg cagaggaata ccaaccattc
    10741 gcacggaacc gcaacggacg ttggcggcaa gttgctgtat gcgttgccag tcatagccgc
    10801 cagcgacgct atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact
    10861 gacccactgc ttgcgcaaaa tcggtgacgt gcaggaaaga gagctgtgta tctggtgcac
    10921 caagtcgtgg cagcaggccg cgcagcatcc agtcaaacag cggttttaac tctttatcgc
    10981 cgggaccata cacggctgtc gggcgaaaaa cgcccagcgt aatttcgtca gccattgcag
    11041 tcagccgttg ttcggcgatg tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg
    11101 ccgccagcga agagataaac agaaaacgtt ggcaaaagca gctctctttt gctgcttgca
    11161 tcaggcgcag gctgccgtca acgttacagc gggtgaaaat ctcttctttg tgcccgcgca
    11221 cttgcccggc gcaatggacc actgcgctgg ctccggcaac cagcttgcta agtgaatgag
    11281 tatcttccag cgaaccgcgc acccaggtga gattatcatt gacgtgagcg cgggcagtac
    11341 gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcgataata tatttaccga
    11401 taaacccggt agcgcccgtc accgcgacag tttgattcat gccagggatt cctgcacatt
    11461 aagactggca gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc
    11521 ggaggacgtt cggggaatac tgtggggcgg caacagatcg atagccgcgg tcacgccaaa
    11581 ttcgctttgg atccgtgccg ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct
    11641 gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc
    11701 gccagaatga atttccggtt cttgttccgc aatatattca atatcctgcg gccagatatt
    11761 acggccacga ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag
    11821 cagataaccg aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaaac
    11881 ctggtcgcca aagtaaccgc tcatcaggct ggggccggag atgcaaatat ggcctaccac
    11941 acgttccgcg accggcatac ctgcttcatt gcggatttca atgccatgtt ccggcaacgc
    12001 tttgccgcag ttgacgaaag tcgatacggc gcgtgtctct gcacccggcg cgacggcttt
    12061 accctgatat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg aggcttcatc
    12121 agagaagctg acagccagcg cattttctgc cagtccgtag cacggcatga aagttttatt
    12181 gtcaaagtta acctgacgga aacattcagc gaattgatgg agttgttctg cggagatggg
    12241 ttctgcacca ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc
    12301 attcacgcgg cgctggcaca attcatagcc aaacggcggc gcaacggaaa cggtgccgcg
    12361 atttttactg atcaatttaa gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa
    12421 ataatctact gaaagctgcg tggcgacggg gggcaggaga aagccgacca gtcccatatc
    12481 atggtagaaa ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa tgccgtcgtg
    12541 gcttatagca cgtagattag ccatcacttc gcgatgggtg ataatgacgc cacggggaaa
    12601 acgggtgctg ccggaggtgt actggaggta ggcgatatcg ttcggaactg gacgctggag
    12661 cgcaacatcg gcttccggta atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg
    12721 gttgttatgc gtcgcggcat tgaccagtgg caaccactca tcaccagtga taatggctgc
    12781 gggctggcaa cttgccagta aaccctggag tttggcgctc caggaatccc gctgaccgac
    12841 gcccattgga atcgccaacg ggacggcgac taagccagca tactgacagg caaaaaaagc
    12901 ctctacaaac ccgctactcg tttcggcaat cagcgccacg cgttcgcctt ttttcaggtt
    12961 cagcgataac aaccgcttcg caccagcttc ggcacgcgtt tttaatgtct gatattccag
    13021 ttgatcttca agttggcaac gtctgtcata aaaattcatt ccggcgctac tccgagcggc
    13081 gtagtccaaa gcatcaacca gcgttggaaa atcggcatag cgcataggta gggaatgcgt
    13141 aaagatttta ttagacatat acaccacgaa aaaa
//
LOCUS       DAZKGS010000126        12849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKGS010000126 DAZKGS010000000
VERSION     DAZKGS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..254
                     /locus_tag="ACHOQY_003577"
     CDS             <1..254
                     /locus_tag="ACHOQY_003577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679609.1"
                     /translation="IQSHIFNPKKNRTKRVNYSGASKNYEEWLSVLSFSENIYPASVS
                     QSKIRLLLNSQKTLHILLISPYRSVTYYSRQTSHLEVKK"
     gene            251..832
                     /gene="mdaB"
                     /locus_tag="ACHOQY_003578"
     CDS             251..832
                     /gene="mdaB"
                     /locus_tag="ACHOQY_003578"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP9679610.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            863..1177
                     /locus_tag="ACHOQY_003579"
     CDS             863..1177
                     /locus_tag="ACHOQY_003579"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9679611.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1225..3117)
                     /gene="parE"
                     /locus_tag="ACHOQY_003580"
     CDS             complement(1225..3117)
                     /gene="parE"
                     /locus_tag="ACHOQY_003580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9679612.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3146..3727)
                     /gene="yqiA"
                     /locus_tag="ACHOQY_003581"
     CDS             complement(3146..3727)
                     /gene="yqiA"
                     /locus_tag="ACHOQY_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9679613.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3727..4554)
                     /gene="cpdA"
                     /locus_tag="ACHOQY_003582"
     CDS             complement(3727..4554)
                     /gene="cpdA"
                     /locus_tag="ACHOQY_003582"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9679614.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4579..5001)
                     /locus_tag="ACHOQY_003583"
     CDS             complement(4579..5001)
                     /locus_tag="ACHOQY_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9679615.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5002..5631)
                     /gene="nudF"
                     /locus_tag="ACHOQY_003584"
     CDS             complement(5002..5631)
                     /gene="nudF"
                     /locus_tag="ACHOQY_003584"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9679616.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5836..7317
                     /gene="tolC"
                     /locus_tag="ACHOQY_003585"
     CDS             5836..7317
                     /gene="tolC"
                     /locus_tag="ACHOQY_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9679617.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7465..8136
                     /locus_tag="ACHOQY_003586"
     CDS             7465..8136
                     /locus_tag="ACHOQY_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9679618.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8142..9302
                     /locus_tag="ACHOQY_003587"
     CDS             8142..9302
                     /locus_tag="ACHOQY_003587"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9679619.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9340..10128)
                     /gene="ygiD"
                     /locus_tag="ACHOQY_003588"
     CDS             complement(9340..10128)
                     /gene="ygiD"
                     /locus_tag="ACHOQY_003588"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9679620.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10271..11044
                     /gene="zupT"
                     /locus_tag="ACHOQY_003589"
     CDS             10271..11044
                     /gene="zupT"
                     /locus_tag="ACHOQY_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9679621.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11102..11272)
                     /gene="yqiD"
                     /locus_tag="ACHOQY_003590"
     CDS             complement(11102..11272)
                     /gene="yqiD"
                     /locus_tag="ACHOQY_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP9679622.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11533..12186)
                     /gene="ribB"
                     /locus_tag="ACHOQY_003591"
     CDS             complement(11533..12186)
                     /gene="ribB"
                     /locus_tag="ACHOQY_003591"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9679623.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12290..12438)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12560..>12849
                     /locus_tag="ACHOQY_003592"
     CDS             12560..>12849
                     /locus_tag="ACHOQY_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFP9679624.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3240 a         3444 c         3159 g         3006 t
ORIGIN      
        1 ttattcaaag ccatattttc aatcccaaaa aaaaccgaac caaacgtgta aattattccg
       61 gtgcgagtaa gaattatgag gaatggctat cagtattgtc attttcagaa aatatttatc
      121 ctgcatcggt gagtcagagc aagatcagac ttttgctaaa ttcgcaaaag actttgcaca
      181 ttttgctaat ttcaccgtac cgctctgtga cgtactatag tcggcaaacg tctcaccttg
      241 aggttaaaaa atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa
      301 tggtcaactg aacgacaccc tgaccgaagt cgcggatggc acactgcgcg acctcgggca
      361 tgatgtccgc atcgttcgcg ccgacagcga ctacgatgtc aaagcggaag tacaaaactt
      421 tctctgggct gatgtggtga tctggcagat gccaggctgg tggatgggcg cgccgtggac
      481 agtgaaaaaa tatattgatg atgtattcac cgaaggtcat ggaacgctgt atgccagcga
      541 tggccgtacc cgcaaagatc cgtcaaaaaa atacggttcc ggcggcctgg tacagggcaa
      601 aaaatatatg ctttctctga cctggaacgc accaatggaa gccttcaccg aaaaagatca
      661 attcttccac ggcgttggcg tcgacggtgt gtatctgccg ttccataaag caaaccaatt
      721 ccttggcatg gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt
      781 tccccgctat actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct
      841 caggctttag aaggagttaa ccatgcttac cgtaatcgca gaaatccgta ctcgtcctgg
      901 tcaacatcac cgtcaggcgg tattggatca gtttgctaaa atcgttccaa ccgtactgaa
      961 agaagaaggt tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca
     1021 gtctatggca ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga
     1081 agcgcatctg caaaccccgc acatgaaggc gtacagcgaa gccgtaaaag gcgacgtgct
     1141 ggagatgaat atccgcatcc tgcaacctgg gatttaaacc tgccttgttt gcccggccat
     1201 cctgaccggg caatgttctt tcctttaaac ctcaatctcc gccatgtcgc ctttctcttg
     1261 caaccagttg cggcgatctt ccgagcgttt cttcgccagc agcatatcca tcatcgcgtc
     1321 agtacgctga tcgtcttcat catcgatagt caactgcacc agacggcgag tgttcggatc
     1381 aagcgtggtt tcacgcaatt gcatcgggtt catttccccc agacctttaa aacgctggac
     1441 gttcggcttg cctttcttgc gttttaattg ctcaagtacg ccctctttct cttcttccgt
     1501 cagcgcgtaa taaacctctt tcccgagatc aatacggtag agcggtggca gtgcgacgta
     1561 aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg aacaaagcgc agagcagcgt
     1621 ggcaatgtgc agaccatcag agtccgcatc cgcgaggata cagattttgc cataacgaag
     1681 ctggctcaga tcgtcgctgt caggatcgat accgatcgct accgaaatat catgcacttc
     1741 ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg ttaaggatct tacctttcag
     1801 tggcatgatc gcctgatatt cgcgatcgcg cgcctgcttg gcagatccgc ctgcggagtc
     1861 accttccaca aggaacagtt cggtacggtt aagatcctgc gcggtacagt cagccagttt
     1921 gccaggcagt gctgggccgc tggtcagctt tttgcgcacc acttttttag ccgcacgcat
     1981 acggcgctgg gcgctggaaa tcgccatctc cgccagcagc tccgccgcct gaacgttctg
     2041 gttcagccac aggataaagg catctttcac cacgcctgaa acgaatgccg cgcattgacg
     2101 cgaagagaga cgctctttgg tctgcccggc aaactgcgga tcctgcattt tcaccgacag
     2161 aacataggcg cagcgatccc agatatcttc cgccgacagc ttcacgccgc gcggcagaat
     2221 gttgcggtat tcacagaact cacgcatcgc gtccaacagg ccctgacgca gaccattaac
     2281 atgggtaccg ccctgcatcg ttgggataag gttgacgtag ctttcggtca gcagttcacc
     2341 gccttccggc agccacagta gcgcccagtc cacagcttca gtatcgccag cgaaattacc
     2401 gataaacggt ttttctggca gcgtcggtaa accgtttacc gcttccgcca ggtaatcatt
     2461 cagaccgtcc tgatagcacc agcgctgttc ggtgttgttg atctcatctt taaaggtaat
     2521 ctcaacgccg gggcacagta ctgctttggc cttcagcaca tgcgtcaggc gtgaaacaga
     2581 aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag tgcacgctgg taccggtatt
     2641 gcgtttaccg caagtgccga caacctgtaa atcctgcacc ttttcgccat tttcaaaggc
     2701 gatgttataa acctgaccat cgcggcgcac gttaacttct acgcgcttcg acagggcgtt
     2761 aaccaccgaa atccccacgc catgcaggcc gccagagaac tggtaatttt tgttagagaa
     2821 tttaccgcct gcatgcagac ggcaaagaat cagttcaacc gccggtacac cctcttccgg
     2881 gtgaatatcc accggcatcc cgcgcccatc gtcaataact tctaacgact ggtcagcatg
     2941 taagattaca tccacgcgtt ttgcgtgacc cgccagtgct tcatcgacac tgttatcaat
     3001 aacttcttgc cccaaatggt tagggcgagt ggtatcggta tacatccccg gacggcggcg
     3061 aaccggctca agcccggtga gtacctcaat ggcatcagcg ttataagttt gcgtcatggt
     3121 ttaagttagc aattcgagtt gatcgtcaga gatggtgcag accaagaaaa tcgacgatcg
     3181 ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc gccttctatg acagtctggc
     3241 ggcaggaagc gtagtacgcc accgcctggc ggtaatccag cacttcatct cccgtctgtt
     3301 gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat ctgcatgact ttgagatcgt
     3361 aaatatggcg tgactctagc acatattgct gcccggtgta ggggttctcg ttctgaccga
     3421 gatagtccgt cagcagttca aacgggcgca ccgccgggtt taccaccact gcgggcagca
     3481 taaaacattg tgacaaccag gtggcgtaat atccccccag tgacgaaccg acaataccca
     3541 gcgaatcgcc gccatgttcc aggacaatgg attccagcag ctctgccgcg tcggaaggat
     3601 acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg atgttccgcc agccagtttt
     3661 ttaacaagct cgcttttgca gagcgcggcg agctgttgaa accgtgtaaa taaagaagcg
     3721 tagacatcag tagccttctg aagcggtatc aggttggaaa cgtgtgtccg ccaggcgatg
     3781 cacctcggtg gtcagcgtgc catcagcatg taactcgaga gtacgccagc cgggcgcgat
     3841 ggtgtccagc gtaaagttgg aacagtgcgg cttaaactgc acacaggtcg acggcgttgc
     3901 cagcaggcgg cgaccgttcc agtcgagatc cagctcctga tgaatatgac cgcacagcaa
     3961 gtatttgacg tgcggaaact tcgccagcac ggtatccagt tcgcccgcgt tacgcaggct
     4021 gtgttgatca agccaactac aacccgcagg tagcggatga tgatgcagca gcagcaacgt
     4081 atggcgttct ggcgcatcgg ccagtttacg ttccagccac tcaagctgaa actcgctcag
     4141 ctcaccgtgc ggcacgccaa acacctggct atccagcaac aggatttgcc attgctcacc
     4201 aataaacacc cgcttcgccg gggagatacc cgcatcctgt aacgcgctgt acatcgcggg
     4261 ctggaaatcg tggttgccag gcagccagac gcagggcgca cgaaaacttg cgatgccttc
     4321 agcgaaatgc cgataggccg cagaggattg atcctgcgct aaatcacctg tcgcgacaat
     4381 caggtcgaat tcgtgctggt gtggacgaat cgcctccagc accgcctggt agctttccca
     4441 ggtatttacc cctaacaggg cttcgtgctt ttgtgcaaac aggtgagtat cggtaatttg
     4501 taaaatcctg actctggcct caccagccag aggaagggtt aacaggcttt ccaaatggtg
     4561 tccttaggtt tcacgacgct agtaaaccgg aatcgccatc gctccatgtg ctaaacagta
     4621 tcgcaaccag tccgctaaaa actggttaat ttgatgcttt tcgtcgcgtt gatgcaactt
     4681 tttattagga taatcatacc gcgctttgaa gcgaaaaatc tgctggcttg aacacacttc
     4741 agccaccatc gcgtcatgat acagacgcac cgtcattgac ggaaggctcc agtaactgat
     4801 cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat cgggtcgatt ccacaatcgt
     4861 cagccgatat tgtgcgtttg ccacctgata gcttacagtt tcgccgggtg cgtcattgcg
     4921 cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac aggcgcatca tttcaggaaa
     4981 gtcaggtgtg taacgcttca tttatgccca ctcatttttt aacgcttgat gatgcagctg
     5041 cagccattgc aaagcgatga ccgacgctgc gttgtcgatt ttcccctctt ctacccactg
     5101 gtatgcctgt tcccggctta ccacatgaac gcgaatatct tcgttttcat cagccagacc
     5161 atgaataccg cttgcggtcg tggcgtccac ttcgcccacc ataattgacg aacgctcact
     5221 ggtgcccccc gggcttgcca ggaaacttaa caccggtttg gtccgtttga ctatcagtcc
     5281 cgcctcttca atcgcttcgc gacgggcaac atcttccaca ctttcaccct cttctatcat
     5341 cccggcaacc atctccagta gccagggggt ttcgctggtg tcgtacgcgg caatccgaat
     5401 ctgctcaatc agcacaactt catcacgcac tgggtcaaag ggtagcaaga ctgcggcgtg
     5461 accgcgctca aaaatttccc gccgtacctc atgactcatt tgcccgttga atagacgatg
     5521 acgaaatcta taaagatcta atgaaaaaaa gccgcgataa agtgtttctc gtgcaataat
     5581 ttctacatcg tttttgccaa atgtaacggg caggttgtct ggcttaagca ttgttaatgt
     5641 cctggcacta atagtgaatt aaatgtgaat ttcagcgacg tttgactgcc gtttgagcag
     5701 tcatgtgtta aattgaggca cattaacgcc ctatggcacg taacgccaac cttttgcggt
     5761 agcggattct gctagaatcc gcaataattt tacagtttga tcgcgctaaa tactgcttca
     5821 ccacaaggaa tgcaaatgaa gaaattgctc cccattctta tcggcctgag cctttctggg
     5881 ttcagttcgt tgagccaggc cgagaacctg atgcaagttt atcagcaagc acgccttagt
     5941 aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg cctttgaaaa aattaatgaa
     6001 gcgcgcagtc cattactgcc acagctaggt ttaggtgcag attacaccta tagcaacggc
     6061 taccgcgacg cgaacggcat caactctaac gcgaccagtg cgtccctgca gttaactcaa
     6121 tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc aggaaaaagc agcagggatt
     6181 caggacgtca catatcagac cgatcagcaa accttgatcc tcaacaccgc gaccgcttat
     6241 ttcaacgtgt tgaatgctat tgacgttctt tcctatacac aggcacaaaa agaagcgatc
     6301 taccgtcaat tagatcaaac cacccaacgt tttaacgtgg gcctggtagc gataaccgac
     6361 gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga acgaagtgac cgcacgcaat
     6421 aaccttgata acgcggtaga gcagctgcgc cagatcaccg gtaactacta tccagaactg
     6481 gcagcgctga atgtcgaaaa ctttaaaacc gacaaaccac agccggttaa cgcgctgctg
     6541 aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac gcttgagcca ggacctggcg
     6601 cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga cgctggattt aacggcttct
     6661 accgggattt ctgacacctc ttatagcggt tcgaaaaccc gtggtgccgc tggtacccag
     6721 tatgacgaca gcaatatggg ccagaacaaa gtgggcctga gcttctcgct gccgatttat
     6781 cagggcggaa tggttaactc gcaggtgaaa caggcacagt acaactttgt cggtgccagc
     6841 gagcaactgg aaagcgcgca tcgtagcgtc gtgcaaaccg tacgttcctc cttcaacaac
     6901 attaatgcat ctatcagtag cattaacgcc tacaaacaag ccgtagtttc cgctcaaagc
     6961 tcattagacg cgatggaagc gggctactcg gtcggtacgc gtaccattgt tgatgtgttg
     7021 gatgcaacca ccacgctgta caacgctaag caagagctgg caaatgcgcg ttataactac
     7081 ctgattaatc agctgaatat taagtcagcc ctgggtacgt tgaacgagca ggatctgctg
     7141 gcactgaaca atgcgctgag caaaccggtt tccactaatc cggaaaacgt tgccccgcaa
     7201 acgccggaac agaatgctat tgctgatggt tatgcgcctg atagcccggc acccgtcgtt
     7261 cagcaaacat ccgcacgcac taccaccagt aacggtcata accctttccg taactgatga
     7321 cgacgacggg gcttcggccc cgtctgaacg taaggcaacg taaagatacg ggttatctgc
     7381 cgcattcttc ccccttctcg cttcaatttc gaccagccat cctctattct gatgggtatt
     7441 taccactggt cccggaagac aaaaatgaaa cggacaaaat ccatacgcca cgcatcgttc
     7501 cgcaaaaact ggagcgcgcg ccatctgaca ccagtcgctc tcgcggttgc cactgttttt
     7561 atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc tctatcaaaa tgctgacgac
     7621 tgttcagctg caaacccagg caaaagcgcc gaatgtacca ccgcgtacaa caatgcgctg
     7681 aaagaagccg aacgtactgc gccgaaatac gccacccgtg aagactgtgt tgctgaattt
     7741 ggtgaaggtc agtgccagca ggcaccagcc caggctggca tggcaccaga aaaccaggcg
     7801 caggcccagc aatccagcgg gagtttctgg atgccgctga tggccggtta catgatgggg
     7861 cgtctgatgg gcggcggcgc gggatttgca cagcagccgc tgttctcctc gaaaaaccca
     7921 gccagcccgg cttacggtaa atataccgac gcgacgggta aaaactatgg cgcagcccag
     7981 ccaggccgca ccatgaccgt accgaagaca gccatggcac caaaaccggc gaccaccact
     8041 accgttaccc gtggcggttt tggtgaatct gttgccaaac aaagcactat gcagcgtagc
     8101 gcaactggca cctcttctcg ttcaatgggt ggctgatacc gatggaaaga gtcagtatta
     8161 ccgagcgccc ggactggcgt gagaaagccc acgaatacgg tttcaatttt cacaccatgt
     8221 acggcgagcc gtactggtgt gaagatgctt actacaagtt gaccctcgcc caggttgaaa
     8281 agctggaaga agtcaccgcc gaactgcacc agatgtgcct gaaagtggtg gaaaaagtga
     8341 tcgccagcga tgagctgatg accaaattcc gcattccaaa acacacctgg agttttgtgc
     8401 gccagtcatg gctgacgcac cagccatcgc tttattcgcg tcttgatctg gcgtgggatg
     8461 gcactggcga acctaaactt ctggaaaata acgccgatac gccaacgtca ctatacgagg
     8521 cggcgttctt tcagtggatc tggctggaag atcagcttaa cgccggtaac ttgccggagg
     8581 gcagcgacca gtttaacagt ctgcaagaaa aactgatcga tcgcttcgtt gagctgcgtg
     8641 aacagtatgg cttccagttg ctgcatctca cctgctgtcg cgacacggtg gaagatcgcg
     8701 gaaccattca gtatttgcag gactgcgcaa cggaagctga aattgctact gagttcctct
     8761 acatcgatga tatcgggtta ggtgaaaaag gtcagttcac ggatttacag gatcaggtaa
     8821 tttccaacct gttcaagctg tatccgtggg aatttatgtt gcgtgagatg ttttccacca
     8881 agctggagga tgcaggcgta cgctggctgg aaccggcgtg gaagagcatt atctccaaca
     8941 aggcacttct accgctactg tgggagatgt tcccgaatca cccgaacctg ctgcccgctt
     9001 attttgcgga agatgatcat ccgcaaatgg aaaaatatgt ggttaaaccg atcttctccc
     9061 gtgaaggcgc aaacgtgtcg atcattgaaa acggcaaaac cattgaagca gcggaaggtc
     9121 cgtatggcga agaagggatg attgttcagc aattccaccc gttaccgaaa ttcggcgaca
     9181 gctatatgct gattggtagc tggctggtga acgatcaacc cgccggaatt ggcattcgtg
     9241 aagaccgtgc attgatcacc caggatatgt ctcggtttta tccacatatt tttgttgagt
     9301 aatcaatgat gccggatggc attcgccatc cggtaattgt taacctatct gcaccgacag
     9361 catactcagg ctgcccattt ctataccatc gaccggaatg gtaattggct cctgcccatc
     9421 ccacgcacct aagacataca acaacggcag atagtgctcc ggcgttgggt tcgataacgc
     9481 gccaccttca tggtcgaggt aattcaccag aggatgctgc tctaccgccc cttgccacgt
     9541 cagattcgct ttcacatact cattaaacga catcgcccaa ggatatggtg aactatcacc
     9601 gtgccacttc actgtacgca ggttatgcac cacgttgcca ctggcgacca acattattcc
     9661 ttcatctcgc agcgctgcca gtttgcgccc catttcgaaa tgccaggcgg caggtttgct
     9721 actgtcgata ctcaactgca ccatcgggat atcggcgtca ggatacatct taatcagcac
     9781 gccccaggaa ccgtggtcaa agccccaggc ttctttatcc agcgtcaccg ggaccggtgc
     9841 taacagctca accagatgct gtgccagcac aggcgaaccc ggagcaggat aatgcgtatc
     9901 gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg ggcggcgtct ccatcgcggt
     9961 cactcctgtt ccacgggtaa accagtgagc cgaaaccacc acaatcgctt gcgggcgtgg
    10021 caatgtcatc cccaacttct gccagctgcg ggtatacaaa ttatcttcca gcacgttcat
    10081 cggactaccg tgacctaaaa acaatgctgg catacgtgtt gaagacatga tgatatcctt
    10141 aactaaaggt gtcattttga tatcctcaca atacgcttgt tcggcggagt aagaacccgg
    10201 ataacaatga tgatgatcat cagttatttt gacgatctgc ctgaaggtga agatttataa
    10261 ggagttgtcg atgtcagtac ctctcattct gaccatactg gcgggggcag ccacgtttat
    10321 tggcgcgttt ctcggcgttc tcgggcaaaa accctcgaac cgcttactgg cgttttcgct
    10381 aggatttgcg gcggggatca tgttgctcat ctcattaatg gaaatgcttc ctgccgcact
    10441 agcagctgaa ggaatgtcgc ctgtgttggg ttatggaatg tttatcttcg gtctgcttgg
    10501 ctattttggc ctggaccgca tgttgccaca tgcgcatccg caggatttaa tgcaaaaatc
    10561 ggtgcagccg ttgccaaaat cgatcaagcg cacagccatt ctgctcactc tcggcatcag
    10621 tctgcataac ttcccggaag ggattgccac ctttgtcacg gcgagcagca acctggagct
    10681 gggatttggc atcgcactgg ccgtcgcgtt gcacaatatt cctgaaggtc tggcggttgc
    10741 aggcccggtt tatgcagcaa cgggttctaa acgcacggca attctgtggg cggggatttc
    10801 tggattggca gaaattcttg gtggtgtgct ggcgtggtta atcctcggta gcatgatttc
    10861 ccccgtggtc atggcggcaa tcatggcggc ggttgcagga attatggtgg cgctctcggt
    10921 tgatgaatta atgccgctcg ccaaagagat tgaccctaat aataacccca gctatggcgt
    10981 actatgtgga atgtcagtga tgggattcag tttagtgctg ctacaaacgg cgggaattgg
    11041 ttaaaagcaa catcagaagt tggccagcgc atggacaact tctgctagct tcactaaagc
    11101 cttattctac cttttcggct gtctcttctc tcgtactgtt taagtatttg ttccgcgctt
    11161 caagcagtgc gtctctgtcc tggcgaaacg cccgacaata acgtttcaaa tgcaggaaat
    11221 aacccaccac aacaagagca atcaatacaa tccagtacca ggcgataaac atccttttac
    11281 ccttaatatt taacaatact aaacatttag cgcataaatt tcacatatcc tttttcggat
    11341 atattcatat ggtcgcataa taacggaaca attatatgat tttgatcata atgaaaaaga
    11401 atgcctgata ttaatgaaat tttctcatag aaagagaagc ggaattaaca tcttgcatag
    11461 cagggcaaaa taaaaaccga agccagctac aacttcggtt tcttgattaa ggcagtaatt
    11521 aagcagcggt tttcagctgg ctttacgctc atgtgcctga cggtatgcca ccaggtcttc
    11581 aatagtcacg agcgccatat tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc
    11641 catcgtgcca tcgtcattag tcaactcaca cagcacacca gccggtttaa agcctgccag
    11701 cgtcatcaaa tcaatagtcg cttcagtatg accgccacgc gtcagtacac cacctgcctg
    11761 cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca gaaggttttg cgccatcggc
    11821 aatcgctgcg cgaacggtcg taattcgatc agcggcagaa acaccggtag tcacaccttc
    11881 agccgcttca atggtcacgg taaaaccggt gccataggcg ctggtgttat tttctaccat
    11941 cattggcaga tcaagttgtt tacggcgatc ttcagtgatg cacaggcaaa caataccgct
    12001 accgtggcga atggtcagcg ccatctgctc aacggtcatg gtttctgccg ggaagatcat
    12061 atcgccttcg ttttcacggt cttcatcatc aagcaccatt acaccgcgtc cttcacgcag
    12121 cgcagccagt gcattttcaa cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg
    12181 attcatggta aaaaaacctc actaaaatta tggttaccag aatcagggca gtcttaggag
    12241 tggcggcata tagccaaaat aacgtgagcg ggtccatgcc cgacagaatc gttactctct
    12301 cccatccgga ctctaaccgt cggccccgga attacaccgg atctgctgtc ctttgagttc
    12361 gcacccaaag cgctcgcggg ctttcaactg agttgattta ccgccggtgg ggaatttcgc
    12421 cccgccctga gaataagcgg attcactata acgctaatga ttagcggcag caacgcatag
    12481 cttcacataa ttctggttta tgacttaccc ttatcgcact acaatggcac tcaacaccta
    12541 tcagtacagg gaaactacaa tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt
    12601 tcacgaatca atgcctaaag gaatcaggga gttcggggaa gatgtggaga aaaaaatccg
    12661 ccaaacccta caagctcagc tgacgcgcct cgaccttgta agccgtgaag agttcgacgt
    12721 ccaaacgcaa gtcctgttac ggactcgtga gaaactggcg ctgcttgaac aaagaatgag
    12781 cgaactggaa aatcgcagta ctgagattaa aaagcagcca gatccagaga ctctcccccc
    12841 aacgctgta
//
LOCUS       DAZKGS010000127        12659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKGS010000127 DAZKGS010000000
VERSION     DAZKGS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..1376)
                     /gene="plaP"
                     /locus_tag="ACHOQY_003593"
     CDS             complement(18..1376)
                     /gene="plaP"
                     /locus_tag="ACHOQY_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9679625.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1366..1428)
                     /gene="yoeI"
                     /locus_tag="ACHOQY_003594"
     CDS             complement(1366..1428)
                     /gene="yoeI"
                     /locus_tag="ACHOQY_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9679626.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1643..2572)
                     /gene="yeeY"
                     /locus_tag="ACHOQY_003595"
     CDS             complement(1643..2572)
                     /gene="yeeY"
                     /locus_tag="ACHOQY_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9679627.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2618..3442)
                     /gene="yeeZ"
                     /locus_tag="ACHOQY_003596"
     CDS             complement(2618..3442)
                     /gene="yeeZ"
                     /locus_tag="ACHOQY_003596"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9679628.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3525..3779)
                     /gene="yoeB"
                     /locus_tag="ACHOQY_003597"
     CDS             complement(3525..3779)
                     /gene="yoeB"
                     /locus_tag="ACHOQY_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP9679629.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3776..4027)
                     /gene="yefM"
                     /locus_tag="ACHOQY_003598"
     CDS             complement(3776..4027)
                     /gene="yefM"
                     /locus_tag="ACHOQY_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP9679630.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4310..4357
                     /gene="hisL"
                     /locus_tag="ACHOQY_003599"
     CDS             4310..4357
                     /gene="hisL"
                     /locus_tag="ACHOQY_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9679631.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    4333..4456
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4503..5402
                     /gene="hisG"
                     /locus_tag="ACHOQY_003600"
     CDS             4503..5402
                     /gene="hisG"
                     /locus_tag="ACHOQY_003600"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9679632.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5408..6712
                     /gene="hisD"
                     /locus_tag="ACHOQY_003601"
     CDS             5408..6712
                     /gene="hisD"
                     /locus_tag="ACHOQY_003601"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9679633.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6709..7779
                     /gene="hisC"
                     /locus_tag="ACHOQY_003602"
     CDS             6709..7779
                     /gene="hisC"
                     /locus_tag="ACHOQY_003602"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9679634.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7779..8846
                     /gene="hisB"
                     /locus_tag="ACHOQY_003603"
     CDS             7779..8846
                     /gene="hisB"
                     /locus_tag="ACHOQY_003603"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9679635.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8846..9436
                     /gene="hisH"
                     /locus_tag="ACHOQY_003604"
     CDS             8846..9436
                     /gene="hisH"
                     /locus_tag="ACHOQY_003604"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9679636.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9436..10173
                     /gene="hisA"
                     /locus_tag="ACHOQY_003605"
     CDS             9436..10173
                     /gene="hisA"
                     /locus_tag="ACHOQY_003605"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9679637.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10155..10931
                     /gene="hisF"
                     /locus_tag="ACHOQY_003606"
     CDS             10155..10931
                     /gene="hisF"
                     /locus_tag="ACHOQY_003606"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9679638.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10925..11536
                     /gene="hisIE"
                     /locus_tag="ACHOQY_003607"
     CDS             10925..11536
                     /gene="hisIE"
                     /locus_tag="ACHOQY_003607"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9679639.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11633..12610)
                     /gene="wzzB"
                     /locus_tag="ACHOQY_003608"
     CDS             complement(11633..12610)
                     /gene="wzzB"
                     /locus_tag="ACHOQY_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9679640.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         3141 a         3331 c         3273 g         2914 t
ORIGIN      
        1 attattagaa aagatgatta tgctacgtct tcgtactgcg gaaccggatt acggaaactc
       61 ttggttacgc aagccaggta aatcagaccg atagcagccc agatcagacc cagaaccatt
      121 gagctttctt ccaggttaac ccacagcgca ccaacggtca gcgcaccaca catcggcagg
      181 aacagatact ggaagtgatc tttcagcgtc ttgttacgct tctcacggat ccagaactgc
      241 gaaataaccg acaggttaac gaaggtgaac gccaccagcg caccaaagtt aatcagcgcc
      301 gtagccatta ccaggtcgaa gttgattgcc agcaaggcaa tcgcgccaac caggatgatg
      361 ttcattgccg gagtacgcca tttcgggtgt acataaccga agaagctttt cgggaacaca
      421 ccgtcacgcc ccattacgta catcagacgc gctacgcctg catgcgccgc cataccggac
      481 gccagtacgg taatggtgga gaagatcagc gcgccaacct ggaacgcttt acccgcaacg
      541 tacagcatga tttcaggctg tgacgcatcc ggatctttaa agcgagagat atccgggaag
      601 tacagctgca ggaagtaagt tgcaaagatg aagatcatgc cgccaatcag tgcggtcagg
      661 aaaatcgcac gcgggatcac gcgctctgca tctttggttt cttccgacag gttgctgatg
      721 ccgtcaaagc cggtaaagga gaagcacagg attgtcgccc cggtaatcat cgggataaca
      781 tgcgcatcgc cagaccagaa cggacgagtg ctcgccagcg tacccgcgcc ttcaccttca
      841 aatacgccat aaacaaccat gcccagaatc accgcgatca gcactacctg caacacgacg
      901 attacagtgt tgaagttcgc tacggatttc agactacgca ggttaaaggc ggtcataaag
      961 gccaccagcg ccaccacaaa catccacgat gggatggaag gcaccagagc ttcaaaatag
     1021 attttcgcca gcagaatgtt gatcatcggc gcgaacagat agtcgagcag agaagaccaa
     1081 cccaccataa agccgacagt cgggctaatg gatttctggg cgtaagtgta tgcagagcca
     1141 gcagaaggat agcggcgaac cagcttcccg tagctcagag ccgtaaacag gatcgcaatc
     1201 aatgcgaacg cataggctgt cggcacatga ccatccgtga ggcctgaaac gataccaaat
     1261 gtatcaaaca gcgtcatcgg ctgcatatag gcaagaccca tcattacaac cggaactaac
     1321 gtaagcgttt tacgcaattc cacgcgagag gtgtttggag taacgttatg cgacatggtc
     1381 attctccttt acggtgataa ccgtcgcgta agcaaaaaat tgccccattt ttttggattc
     1441 ctcagcgaca acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct
     1501 tggtatgaat gattggtttg aagcaaaaaa taaccgacgc taattaaacg tcggttttta
     1561 gtcatttttt gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga
     1621 ggcttgtcat aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa
     1681 caccggcgat cgcactattt gctaaaattt cgtcccgcca ccaaccactg gcaagccccg
     1741 cggtctgttc attccagcca atccagacgg gttcaaagag ggtttgggcg accactttct
     1801 tctcgattaa cgcaccacta tcgagaaaac gttgcgccag ataacggggt aaatagccgc
     1861 agccgaggcc gctaatttgc agctccagct tggttttaaa atcgaaaacg gtaatagctt
     1921 cttgttcgtc gagaagttgt gaggccgtag aagcgccggc ctgtgcagtg tcccccacca
     1981 caatcgcccg atatcgttta atgatacgac ggtttaacgg ttcttcttcc agggctaacg
     2041 gatgatgagg cgcgaccgca aagacttgtt ccagatcgcc caaccgagag aaaccaaact
     2101 cactggaaga tggtggctca tgcatcgcgc caacaataat atccgctcgc ccctgagtca
     2161 gggcatccca tgaaccaccg agtacaccat tgataaactt caggcgcgtc acgctatgat
     2221 gttgataaaa agcctcaatg agtggcgcaa gcagagaaaa agggaaggtg tcgtccacgc
     2281 caatcaccag ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc
     2341 gcacggtatg cagaacttcc cgccctttct ctaataacat ttttccggtg cgggtgaatt
     2401 tagcgcggtg tccgctgcga tcaagcaatt gaatattaag gtcgctttcc agcttgtgaa
     2461 cggtgtaact cagagcggac ggcgtcttgt agagtttggc cgacgccgcc gcaaaactgc
     2521 cttctttttc taaggcatcg aggatcatca acacatccag cagtggtttc atacacgccc
     2581 ccttgcagtg tatgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg
     2641 gatcgggata ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct
     2701 tacctttgcc gctgtccaga ctatttctga actgcggagg ttccagcccc aataaacggg
     2761 ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt
     2821 gtccgccttt aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga
     2881 ctaaattaac accatgttca ccatcagggg cggtttttcc ggcaaagaag cgtccgggat
     2941 gacgtcccgg tccgaccagg cccgcaagac gcagaatatc gaccgaagta ccgggtaaat
     3001 tgtgcagcca gtcttcgagt tcttctaata ctcgtccact gttggtcacc ggattacgcg
     3061 gggtggtttc tttcaccgtg ccttgcgcgt cgccatagac agatgtcgag ctggtaaaaa
     3121 taatgcgggg aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat
     3181 agaactcatc gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat
     3241 ccatcagggc atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat
     3301 agctatcaat gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg
     3361 tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg catgcctaac caccctaacc
     3421 cgacaattgc gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac
     3481 gctagcgtat caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga
     3541 catgctgcaa tgagcagtga atcatcagta accgcgtata ccagacggtg ctcctctgta
     3601 atgcgtcggg accagaaacc tgacaaatta tgtttcaggg gttctggttt ccccttacct
     3661 tcaaatggtg ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta
     3721 tctgtttcct gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
     3781 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
     3841 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
     3901 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
     3961 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
     4021 tacgcatgta aatctcctct tttgtacagt tcattgtaca atgatgggtg ttaattcact
     4081 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
     4141 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttt cccgctcatt
     4201 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
     4261 tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta tgacacgcgt
     4321 tcaatttaaa caccatcatc accatcatcc tgactagtct ttcaggcgat gtgtgctgga
     4381 agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga agatcacctt
     4441 ccgggggctt ttttattgcg cggttgttaa cggttcagac aggtttaaag aggaataaga
     4501 aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt ttaagtgatg
     4561 actcacgcga attactggcg cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga
     4621 tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgat gacattcccg
     4681 gtctggtaat ggacggcgtg gtagaccttg ggattatcgg cgaaaacgtg ctggaagaag
     4741 agctgcttaa ccgccgcgcc cagggtgaag atccgcgcta ctttaccttg cgtcgtctgg
     4801 atttcggcgg ctgccgcctt tcgctggcaa cgccggttga tgaagcctgg gacggtccgc
     4861 tctccttaaa tggtaaacgt atcgccacct cttatcctca cctgctcaag cgttatctcg
     4921 accagaaagg tatctctttt aaatcctgct tactgaacgg ctctgtcgaa gttgcgccgc
     4981 gtgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg ctggaagcta
     5041 acggcctgcg cgaagtcgaa gttatctacc gctcgaaagc ctgcctgatc cagcgcgatg
     5101 gcgaaatgga agaatccaaa cagcaactga tcgacaaact gctgacccgt attcagggtg
     5161 tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgaccgaa cgtctggatg
     5221 aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat tctgccgctg gcgggtgacc
     5281 aacaacgcgt agcgatgcac atggtcagta gcgaaaccct gttctgggaa accatggaaa
     5341 aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc gattgagaag atgatggagt
     5401 gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcgg agcaacaacg
     5461 ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca ccgttaacga
     5521 tattctcgat aacgtgaaag cacgcggcga tgacgccctg cgggaataca gcgcgaagtt
     5581 tgataaaacc acggttaccg cgctgaaggt gtctgctgag gagatcgccg ccgccagcga
     5641 acgccttagc gacgagctaa aacaggcgat ggcggtggca gtaaaaaata ttgaaacctt
     5701 ccacactgcg caaaaactgc cgccggtaga tgttgaaaca cagccaggcg tgcgttgcca
     5761 gcaagtcacg cgcccggtag cttcagttgg gttgtatatt cctggcggct ccgccccact
     5821 cttctcaacg gtattaatgc tggcaactcc tgcgcgtatt gcgggctgta aaaaagtggt
     5881 gttgtgctca ccgccgccga ttgccgatga gatcctttat gcggcgcagc tgtgcggtgt
     5941 gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc gctctggcgt ttggtacgga
     6001 atctgtgccg aaagtggaca aaatcttcgg gccgggtaac gcctttgtca ccgaagcgaa
     6061 acgtcaggtg agccagcgtc aggacggtgc ggcgatcgat atgcccgcag gcccgtcgga
     6121 agtgctggtg attgctgaca gcggcgctac gccagatttc gtggcttctg atttgctttc
     6181 tcaggctgaa cacggcccgg actcacaggt gattttactg acgcccgctg ctgatatggc
     6241 gcgtcgcgtt gccgaggccg tcgaacgcca gttggcagaa ctgccgcgtg ccgaaaccgc
     6301 ccgccaggca ctgaacgcca gccgcctaat cgtgaccaac gatttagcac agtgcgtgga
     6361 gatctccaac cagtacggcc cggagcacct gatcattcag acccgcaacg ctcgcgatct
     6421 ggtcgatagc atcaccagcg ccggttcggt atttcttggt gactggtcac cggaatcggc
     6481 aggtgattac gcctccggca ccaaccacgt tctgccgact tacggttaca ccgccacctg
     6541 ttccagcctc gggctggcag atttccagaa gcgcatgacc gtgcaggaac tgtcgaaaga
     6601 aggcttttct gcgctggctt caaccataga aacactggcc gccgccgagc gcctgaccgc
     6661 ccataaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat gagcaccgtg
     6721 actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca gtcggcgcgt
     6781 cgtctgggcg gtaacggcga cgtctggctg aacgccaacg aatatcccgc agccgtggag
     6841 tttcagctta ctcaacaaac gctcaaccgc tacccggaat gccagccgaa agcggtgatt
     6901 gaaaattacg cgcaatatgc aggcgtaaaa ccggagcagg tgctggtcag ccgtggcgcg
     6961 gacgaaggta ttgaactgct gattcgcgct ttttgcgaac cgggtaaaga cgccatcctc
     7021 tactgcccgc caacgtacgg catgtacagc gtcagcgccg aaacgattgg cgtcgagtgt
     7081 cgcacagtgc cgacgcagga caactggcaa ctggacttac agggcatttc cgacaagctg
     7141 gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc cgaccgggca actgatcaat
     7201 ccgcaggatt ttcgcactct gctggagcta acccgcggaa aagccattgt ggttgccgat
     7261 gaagcctata tagagttttg tccacaggca tcgctggctg gctggctggc ggaatatccg
     7321 cacctggcta ttttgcgcac actatcgaaa gcttttgctc tggcggggct tcgttgcgga
     7381 tttacgctgg caaacgaaga agtcatcaac ctgctgatga aagtgatcgc cccctacccg
     7441 ctctcgacgc cggttgccga cattgcggcc caggcgttaa gcccgcaggg gatcgtcgcc
     7501 atgcgcgaac gggtggcgca aattattgca gaacgcgaat acctgattgc cgcactgaaa
     7561 gagatcccct gcgtggagca ggtttttgac tctgaaacca actacattct ggcgcgcttt
     7621 aaagcctcca gtgcagtgtt taaatctttg tgggatcagg gcattatctt acgtgatcag
     7681 aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg tgaagaaagc
     7741 cagcgcgtca ttgacgcctt acgtgcggag caagtttaat gagtcagaag tatcttttta
     7801 tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg gaccgttttg
     7861 acaaactcgc ctttgaaccg ggcgtgatcc cggagctgct gaagttgcaa aaagcgggct
     7921 acaaactggt gatgatcact aatcaggatg gtctgggaac acaaagtttc ccgcaggcgg
     7981 atttcgatgg cccgcacaac ctgatgatgc agatcttcac ctcgcaaggc gtacagtttg
     8041 atgaagtgct gatttgtccg cacctgcccg ccgatgagtg cgactgccgt aagccgaaag
     8101 taaaactggt ggagcgttat ctcgctgagc aagcgatgga tcgtgccaat agttatgtga
     8161 ttggcgatcg cgcgaccgac attcaactgg cggaaaacat gggtattaat ggtttacgct
     8221 acgaccgcga aaccctgaac tggccaatga ttggcgagca actcactaaa cgagaccgtt
     8281 acgcccatgt agtgcgtaac accaaagaga cgcaaattga cgtccaggtg tggctggatc
     8341 gcgaaggtga cagcaagatt aacaccggcg ttggcttctt tgatcacatg ctggatcaga
     8401 tcgccaccca cggcggtttc cgtatggaaa tcaacgtcaa aggcgacctc tatatcgacg
     8461 atcaccacac agtcgaagat accggcctgg cgctgggtga agcgttaaaa attgcccttg
     8521 gcgacaaacg cggtatttgc cgctttggtt ttgtgctacc gatggacgaa tgccttgccc
     8581 gctgcgcgct ggatatctct ggtcgcccgc acctggaata taaagccgag tttacctacc
     8641 agcgcgtggg cgatctcagc accgaaatga tcgagcactt cttccgttcg ctctcttaca
     8701 ccatgggcgt gacgctacac ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaga
     8761 gcctgttcaa agcctttggt cgcaccctgc gccaggccat ccgcgtggaa ggcgacaccc
     8821 tgccctcgtc aaaaggagtg ctgtaatgaa cgtggtgatc cttgataccg gctgcgccaa
     8881 tctgaactcg gtgaagtctg ccattgcgcg ccacggttat gaacccaaag tcagccgtga
     8941 cccggacgtc gtgttgctgg ccgataaact gtttttaccc ggcgtaggca ccgcacaagc
     9001 agcgatggat caggtgcgtg agcgcgaact gtttgatctc atcaaagcct gtacccaacc
     9061 ggtgctggga atctgcttag ggatgcaact gctggggcgg cgcagcgaag agagcaacgg
     9121 cgtcgacttg ctgggcatca ttgacgaaga cgtgccgaaa atgaccgact ttgctctgcc
     9181 actgccacat atgggctgga accgcgttta tccgcaggca ggcaaccgcc tgtttcaggg
     9241 aattgaagac ggcgcgtact tttactttgt tcacagttac gcaatgccgg tcaatccgtg
     9301 gaccatcgcc cagtgtaatt acggcgaacc gttcaccgcg gcggtacaaa aagataactt
     9361 ctacggcgtg cagttccacc cggagcgttc tggtgccgct ggcgctaagt tgctgaaaaa
     9421 cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac tgtggtgcgt
     9481 ctccatcagg gcgattacgg caaacagcgc gattatggta acgacccgct gccgcgattg
     9541 caggattacg ccgcgcaggg tgcagaattg ttgcatctgg tggatctgac cggggcaaaa
     9601 gatccggcta aacgtcaaat cccgctgatt aaaaccctgg tggcgggcgt taacgtgccg
     9661 gtgcaggttg gcggtggcgt gcgtagcgaa gaagacgtgg cggcgttact ggaagcaggc
     9721 gttgcgcgcg tggtggttgg ctccaccgcg gtgaaatcac cagagatggt gaaaggctgg
     9781 tttgaacgtt tcggtgccga tgccttagtg ctggcgctgg atgtccgtat tgacgagcaa
     9841 ggcaacaagc aggtggcagt cagcggctgg caagagaact cgggcgtttc actggaacaa
     9901 ctggtggaaa gctatctgcc cgtcggcctg aaacatgtgc tgtgtaccga tatctcgcgc
     9961 gacggcacgc tggcaggttc taacgtctct ttatatgaag aagtgtgcgc cagatatccg
    10021 caggtggcgt ttcagtcctc cggcggtatt ggcgacattg atgatgtggc ggccctgcgt
    10081 ggtactggcg tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa attcaccgtg
    10141 aaggaggcca tcgcatgctg gcaaaacgca taataccgtg tctcgacgtc cgtgatggtc
    10201 aggtggtgaa aggcgtacag tttcgcaacc atgaaatcat tggcgatatc gtgccgctgg
    10261 caaaacgcta cgctgaagaa ggtgcagacg aactggtgtt ctacgatatc accgcttcca
    10321 gcgatggccg tgtggtagat aaaagctggg tatctcgcgt ggcggaagtg atcgacattc
    10381 cgttctgcgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt ctttcctttg
    10441 gcgcggataa aatttccatc aactcccctg cgctggcaga cccaacatta attactcgcc
    10501 tggcggatcg ttttggcgtg cagtgtattg tggtgggtat tgatacctgg tacgacgctg
    10561 aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc cgcgtcactc
    10621 agtgggaaac actcgactgg gtacaggaag tgcaaaaacg cggtgccgga gaaatcgtcc
    10681 tcaacatgat gaatcaggac ggcgtgcgta acggttacga cctcgagcag ctgaaaaaag
    10741 tgcgagaagt ttgccgcgtc ccgctgattg cctccggtgg cgcgggcacc atggaacact
    10801 tcctcgaagc cttccgtgat gccgacgttg acggcgcgct ggcggcttcc gtattccaca
    10861 aacaaataat caatattggt gaattaaaag cgtacctggc aacacagggc gtggagatca
    10921 ggatatgtta acagaacaac aacgtcgcga actggactgg gaaaaaaccg acggactgat
    10981 gccggtgatt gtgcaacacg cggtatctgg cgaagtgcta atgctgggct atatgaaccc
    11041 ggaagcctta gacaaaacca tcgaaagcgg caaagtcact ttcttctcgc gcactaaaca
    11101 gcgactgtgg accaaaggcg aaacgtcggg caatttcctc aacgtagtaa atattgcgcc
    11161 ggactgcgac aacgacacgc tactggtact ggcgaatccc atcgggccaa cctgccacaa
    11221 aggcaccagc agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga
    11281 acaattgctc gccgaacgca aatctgccga tccggaaacc tcttacacag ccaaactgta
    11341 tgccagcggc accaaacgca ttgcacagaa agtgggcgaa gagggcgtgg aaaccgcact
    11401 agcagcaacg gtacatgacc gctttgaact caccaacgag gcgtctgact tgatgtatca
    11461 cctgttggtg ttgttgcagg atcaggatct ggatttaaca acggtgattg agaacctgcg
    11521 taaacggcat cagtgagttg cggggtaagc ggatgcgatg ttgttgtcgc atccggcaaa
    11581 aaaaacgggc aaggtgtcac caccctgccc gttttcttta aaaccaaaaa gattacttcg
    11641 cgttgtaatt acgcagagcg ttacgcccca gcacaatccc cgcgccaacc atgccgccca
    11701 gcagcaccgc cagaatcaaa gtgattgcct ttttcgggct atcgcgacga ataggtaacg
    11761 tcggtttcat cacatagcgg taagcatgaa tatcaagatc atcaactttt aagctttcga
    11821 tatccagcag gttttgacgt gtctgatagt agtttggtga gaacaccaac ggacgagtcg
    11881 cttcatgctt aatcatcgac tccagcgctt cgctccccaa aaggaacaac gtatcttgtg
    11941 tcacatcttc agtctgctga acctgtggct ttgtcacctg cgcctgattc gcatactgca
    12001 acgcttcctg aatctgacgg atacgcagat ctttctgctc ctgcgcaact acttcctgag
    12061 ttttcagaga atcctgcaag tttttcatac gcagcgcgat gttgtctttc aggtcttttc
    12121 ccagttcttc atttacctgg tcgtcaactt gctggatata cttcgccagc tgcttctgag
    12181 caccttcagg tgtttcacca acataagaca ccgctaaagg taatgactga tttttgacgg
    12241 taggctcaat ggtcagcttt tctgcttctt cctggttatc cagcgtttcc gctaatgctg
    12301 agaatgcagt gctgtaacgt ccgataagtg aagcctgaat gtcggagact ttcggtacag
    12361 ccgatccgta gatcacattc atcgcgttgg tataaccagc gatttgcccc acatcaggtt
    12421 gagtaacgat agctgtggaa gtccactttt ctttggcaac ggccagatac cctatagcca
    12481 gggcaatagc aacaatgaca gagatgataa ttgtcatctt gccacgccac aactgcacca
    12541 gtaaatcaat caaatcaatc tgttccgggt catggttttg cccagaaaca ttattatttt
    12601 ctactctcat cattacccta acggacgaaa taattttaag tcgccatagt ttagcgata
//
LOCUS       DAZKGS010000128        12505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKGS010000128 DAZKGS010000000
VERSION     DAZKGS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(55..336)
                     /gene="yehE"
                     /locus_tag="ACHOQY_003609"
     CDS             complement(55..336)
                     /gene="yehE"
                     /locus_tag="ACHOQY_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP9679641.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(599..1708)
                     /gene="apbC"
                     /locus_tag="ACHOQY_003610"
     CDS             complement(599..1708)
                     /gene="apbC"
                     /locus_tag="ACHOQY_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9679642.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1840..3873
                     /gene="metG"
                     /locus_tag="ACHOQY_003611"
     CDS             1840..3873
                     /gene="metG"
                     /locus_tag="ACHOQY_003611"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9679643.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4014..7808
                     /locus_tag="ACHOQY_003612"
                     /pseudo
     CDS             4014..7808
                     /locus_tag="ACHOQY_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7818..11450
                     /gene="yehI"
                     /locus_tag="ACHOQY_003613"
     CDS             7818..11450
                     /gene="yehI"
                     /locus_tag="ACHOQY_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9679644.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11512..11829
                     /gene="yehK"
                     /locus_tag="ACHOQY_003614"
     CDS             11512..11829
                     /gene="yehK"
                     /locus_tag="ACHOQY_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP9679645.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3279 a         3168 c         3058 g         3000 t
ORIGIN      
        1 tatttatata aatgatttcg gctttctaaa tgcaattaaa atcaggttgc caaattaatc
       61 gtagcgattt aacacgattc gacgtttgct atcgctacct gcaacaacca cttctgtcgt
      121 tacacctttg acgggcgaag aaaacagatg acgggtgtca acattatacg ttaactgacc
      181 acaatgctgt tgaggttgac cattaaccat agccatactg catgttgggg gactgatcct
      241 gccattaatc gctaatgtgg cagtctctga agagaatgcg ggagaagcca atccataggc
      301 aagaaaaata attcctgaca gccaatactt attcattgaa cgttatccct gtagtaaagg
      361 ttatgcctgg atagaatgag tgcataacaa actatagctg tacatccact acacagccac
      421 gaaggatgat aatgaagcat tgcctgtatg atcaatcgac tttgtagagt ttcggacgaa
      481 ggtccgcaga atattcgcag tattaaataa gtcttcatta atgcggcaaa aactaataca
      541 ccgcatcaat gtaacatctc tgcggtgtat gaagaataac cagaatggtt aataggcgtt
      601 agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc ctgccagtag agctgagctg
      661 caacgcggtc agccagctgg cggtagatag cggtaaattc gctctccgga cggctgataa
      721 ccgttggcgt gcctttatcg agatcctcac gtaaggagat gtgtaacggc atctgcccca
      781 gcagctgtgt gtggtatttt tcagccagtt tctcagcacc accggtaccg aaaatcggtt
      841 catggtgacc acagttactg caaatatgca cactcatgtt ctcgacaata ccaagcaccg
      901 gaacctcgac tttctcgaac atcacaatgc cttttttcgc atcgatcaac gcgatgtcct
      961 gcggggtggt gaccacgact gcaccagtta ctggaatgtt ttgcgccagc gtcaattgaa
     1021 tgtcaccggt gcctggcggc atatcaagca ccagatagtc gagatccggc cacaaggttt
     1081 cctgcaacat ctgcattaac gccttgctcg ccatcggtcc acgccacacc atcgcattgt
     1141 cgtcagtgac cagataacca attgaattgg ttgccaggcc atgagacata atcggtgcca
     1201 tgtgggtacc gtccggcgac gttggacgct gattttctgc gcccagcatg gtgggaattg
     1261 atgggccgta gatatcggcg tccagaatgc cgactttcgc cccttccgcc gccagtgcca
     1321 gcgccaggtt taccgccgtg gaggatttac ccacgccgcc tttaccggaa ctgaccgcaa
     1381 tgatgttctt cacaccatta atacccggct gattcttcac gcgttttaag gtggcgatat
     1441 tgtgcgacag tttccagtcg atagctttcg ccccggtgat acgcagcagt tcggcgctac
     1501 attgctcttt taactcttca aacgcgctat gccagacgaa tggcataacc agttccacat
     1561 gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt cagcgtggtg aggttatgct
     1621 tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac cattgcgcgc aaggcttctg
     1681 gtgatttggc ctgggattgt tcgttcatcc cgactccttt tttgtataga taaaccatca
     1741 gctgatagtt tacctgaaga atatagagaa gtacttactt aacattttcc catttggtac
     1801 tatctaaccc cttttcacta ttaagaagta atgcctacta tgactcaagt cgcgaagaaa
     1861 attctggtga cgtgcgcact gccgtacgct aacggctcaa tccacctcgg ccatatgctg
     1921 gagcacatcc aggctgatgt ctgggtccgt taccagcgaa tgcgcggcca cgaggtcaac
     1981 ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc tgaaagctca gcagctcggt
     2041 atcaccccgg agcagatgat tggcgaaatg agtcaggagc atcagactga tttcgcaggc
     2101 tttaacatca gctatgacaa ctatcactcg acgcacagcg aagagaaccg ccagttgtca
     2161 gaacttatct actctcgcct gaaagaaaac ggttttatta aaaaccgcac catctctcag
     2221 ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt ttgtgaaagg cacctgcccg
     2281 aaatgtaaat ccccggatca atacggcgat aactgcgaag tctgcggcgc gacctacagc
     2341 ccgactgaat tgatcgagcc gaaatcggtg gtttctggcg ctacgccggt aatgcgtgat
     2401 tctgaacact tcttctttga tctgccctct ttcagcgaaa tgttgcaggc atggacccgc
     2461 agcggtgcgt tgcaggagca ggtggcaaat aaaatgcagg agtggtttga atccggcctg
     2521 caacagtggg atatctcccg cgacgcgcca tactttggtt ttgaaattcc gaacgcgccg
     2581 ggcaaatatt tctacgtctg gctggacgca ccgattggct acatgggttc tttcaagaat
     2641 ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat actggaagaa agactccacc
     2701 gccgagctgt accacttcat cggtaaagat attgtttact tccacagcct gttctggcct
     2761 gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc tgtttgttca tggctatgtg
     2821 acggtgaacg gcgcaaagat gtccaagtct cgcggcacct ttattaaagc cagcacctgg
     2881 ctgaatcatt ttgacgctga cagcctgcgt tactactaca ctgcgaaact ctcttcgcgc
     2941 attgatgata tcgatctcaa cctggaggat ttcgttcagc gtgtgaatgc cgatatcgtg
     3001 aacaaagtgg ttaacctggc ctctcgtaat gcgggcttta tcaacaagcg ttttgacggc
     3061 gtgctggcaa gcgaactggc tgacccgcag ttgtacaaaa ccttcactga tgccgctgaa
     3121 gtgattggtg aagcgtggga aagccgcgaa tttggtaaag cagtgcgcga aatcatggcg
     3181 ctggctgatc tggctaaccg ctatgtcgac gagcaggctc cgtgggtggt ggcaaaacag
     3241 gaaggccgcg atgccgacct gcaggcaatt tgctcaatgg gcataaacct gttccgcgtg
     3301 ctgatgactt acctgaagcc ggtactgccg aaactgaccg agcgcgcaga agcattcctc
     3361 aatacagaac tgacctggga tggtatccag caaccgctgc tgggccacaa agtgaatccg
     3421 ttcaaagcgc tgtataaccg catcgatatg aagcaggttg aagcactggt ggaagcgtct
     3481 aaagaagaag tgaaagcagc tgccgcgcca gttactggcc cactggcaga tgatccgatt
     3541 caggaaacca tcacctttga cgacttcgca aaagttgacc tgcgcgtggc gctgatcgaa
     3601 aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc tgacactgga tctcggcggt
     3661 gaaaaacgca atgtcttctc cggtattcgt tctgcctacc cggatccgca ggcactgatc
     3721 gggcgtcaca ccattatggt ggccaacctg gcaccgcgta aaatgcgctt tggtatctcc
     3781 gaaggcatgg taatggctgc tggtcctggc gggaaagata ttttcctgct aagcccggat
     3841 gccggtgcta aaccgggtca tcaggtgaaa taatccccct tcaaggcgct gcatcgacag
     3901 cgccttttct ttataaattc ctaaagttgt tttcttgcga ttttgtctct ctctaacccg
     3961 cataaatact ggtagcatca gcattcaact ggataaaatt acagggatgc agaatgaaac
     4021 actttatcta tcaggacgaa aaatcacata aattctgggc agttgagcaa cagggaaacg
     4081 agttgcatat cagttgggga aaagttggca ccaaagggca aagccagata aaaagttttt
     4141 cagatgctgc ggcagcggca aaagcggagc ttaagctgat tgcggagaag gtgaagaagg
     4201 ggtatgtgga gcaagcgaag gataattctt tgcaaccttc ccaaacggta acgggctctc
     4261 tcaaggtagc ggacttatcc accattattc aggaacaacc ctctttcgta gcagaaaccc
     4321 gtgcgcctga caaaaataca gatgctgttt taccgtggct ggcgaaagat attgctgtcg
     4381 tttttccgcc cgaagttgta cacaccacgt taagtcatcg ccgctttccc ggagttcctg
     4441 ttcagcaagc agacaaattg acccaattac gtcgcttagc ctgtagtgtg tcgcaacggg
     4501 ataataaaac agccacattt gacttcagcg cctgttcttt agaatggcaa aacaccgtcg
     4561 cccaggcgat cagtcagatc gacggcctga aaacaacaca gctaccatca ccagtaatgg
     4621 ctgtactcac ggcacttgaa atgaaatgca caagatataa agtgcgtgag gatgttatgg
     4681 atcagatcgt ccaggaaggc ggtctggaat atgctactga tgtaataata caccttcaac
     4741 agattgatat taaatgggat tatgcgaata atgtcattat tattctgccg tctggcattg
     4801 cacctgacta cttggagcaa tattccagat ttgaattacg cctacgtaaa catttatcac
     4861 tggcggaaga gtctctctgg caaaaatgtg cacaaaaact tattgccgca attccacata
     4921 ttccagaatg acggcaacca ttaattgctt tgttattacc tgaaaaacca gaaattgcac
     4981 atgaaattgc ccagcgtcta ctggggcaaa aaaaattacc ctcgcttgag tggttaaaaa
     5041 tagtggcgac tgatgagcac attcttgcct cattagaaaa atatcacgag ccatatgcca
     5101 tttttgatga ttactattgt ggtgcgatat ggtcagccac cgtattacag gagcaaggtg
     5161 ttgcagccct gccccgattt gctccctatg ccgcaagtga ctactgcgcc gatgtgttgc
     5221 gtcatatcaa tcatccgttc gcattaacac tgcttatacg tgtagccggg catactaaac
     5281 gctgtcacga tcggatgacg aaagccattg ctgcgttccc acatgcagca atggcggcac
     5341 tgacggaact tcttgggcaa aaagaagaga acagttggcg cattatgcta atgacaatgc
     5401 ttatctcaca accagcactg gcagaacagg tcattccctg gctctcgaca cccgcagttg
     5461 ccgtactgaa atcatgccag caacaactga cacagccctc aaaccatgcc agcgccgatc
     5521 tactgccagc cgtagtagtc tcccctccct ggctttcgaa aaagaaaaaa tcgccgattc
     5581 cggtgctgga tttagcgcca ttaggcattg agccaatctg ttatctgaca gaagaaatca
     5641 gtaatcaact tttggcgaaa tatatctggt attcaaaaca catcacggtt agccatgaag
     5701 aaagtactac caacctgttg gcaaggatgg gttttcaacg acggatcgct ggtacatata
     5761 ttaaagctcc cgaagcggta gttgaggcat ggctaaatga agattattca accttactaa
     5821 gtgaatttaa ggtgtttcat tcacctaccg ggcattattg gcagttgggg attttgacaa
     5881 cattgccgct ggagaaagca gtaaaagcat ggaatgccct taccctatct ccacataccg
     5941 ataccgaata cgccatgtta cattttggac tcaaagggtt acctgggtta gtaaactcac
     6001 ttgcacgcta tccacaagaa gccttgccca tcacgaatta cttcgcagcg agtgagctgg
     6061 ctcctgccgt cgcccgtgcc ttcaacaaac tgaaaacgct acgcgaaaac gcccgtagct
     6121 ggctgttgaa atacccggaa catgccctta ccggcctgct gcctgcggcg ctcggcaaag
     6181 ccggtgaagc acaggataac gcccgcgctg ccttgcgtat gcttaccgaa aacggtcatc
     6241 agccattact gcaagaaatc gcccgacgtt ataaccagcc ggaagtaacc gatgcggtga
     6301 acgctctgct tgcgctcgat cccttagata atcacccgac aaaaatcccc actcttccgg
     6361 ccttttatca gccatcgctc tggacgcgcc cggtattaaa agcaaatgcc caatcactgc
     6421 cagatagcgc cctcctccac ctcggtgaaa tgctccgctt ccctcaggaa gaggctctgt
     6481 atccgggatt attgcaggtg aaagacgtct gttccgccga ctcactggcg ggatttgcct
     6541 gggatctgtt taccgcctgg cagaccgctg gcgcgccgtc gaaagagagt tgggcgttca
     6601 ctgcgttagg cgttctcggt aacgatgaca ccgcccgcaa actgacgcca ttaatacgcg
     6661 cctggcctgg tgaatcccag cataaacgcg ccaccgttgg gttggatatt ctcgctgcta
     6721 tcggtagtga tatcgccctt atgcagctta acggcatcgc ccagaaactg aaattcaaag
     6781 cattacagga gcgggcaaaa gaaaaaattg ccgacattgc cgagagccgc gaactcacgg
     6841 tggcggagct tgaagatcgg ttagcaccgg atctcggtct ggatgataac ggttcgctgc
     6901 tgctggattt tggcccacgg cagttcaccg tcagctttga tgaaacctta aaaccgtttg
     6961 tgcgtgatgt ttccggcagc cgcctgaaag acctgcccaa accgaacaaa agcgatgatg
     7021 aatcgcggtc gaacgatgcg gttaaccgct acaaattgct gaaaaaagat gcgcgtaccg
     7081 tcgccgccca gcaggtagca aggctggaat ccgccatgtg cctgcgccgc cgctggtcgc
     7141 cggaaaactt ccagctcttc ctggttgagc atccgctggt tcgccactta acccgccgtc
     7201 tgatttgggg cgtttatagc gccgaaaacc agctactggc ttgctttcgc gtagcagaag
     7261 ataacagcta cagcaccgct gacgatgatc ttttcaccct gccggaaggc gatatctcta
     7321 tcggcattcc tcacgttctg gagatatcac cgacggatgc cgccgccttt gggcagcttt
     7381 ttgccgacta tgaactgtta ccgccgttcc gccagctcga ccgtaacagt tacgccctga
     7441 cagaaaccga gcgcaatgcc agcgaattga cccgctgggc aggcagaaaa tgcccgagcg
     7501 gtcgggtcat gggactggcg aataaaggtt gggtgcgtgg tgaaccgcag gatggtggct
     7561 ggatcggctg gatgatcaaa cctctgggcc gctggtcgtt aatcatggaa atcgatgaag
     7621 gctttgcagt tggtatgtcg ccagccgaac ttagcgccga gcaactctta agcaagctgt
     7681 ggctatggga aggcaaagca gaaagctatg gctgggggag taattcaaca caggaagcgc
     7741 agttctccgt actcgatgcc atcaccgcca gcgagctaat taacgatatt gaagccctgt
     7801 ttgaataagg aaagagcatg gacaaggaat taccgtggct ggcggataac gcccaactgg
     7861 aactgaaata taaaaaaggc aaaacgccgc tcagtcatcg tcgctggccg ggcgaaccag
     7921 tgtccgttat cactggaagt ctcatccaga cattgggaga tgaattacta caacaagcag
     7981 gccagaagga aaatatcacc tggaattatg ataaatgctc actggagtgg cagtccgcca
     8041 tccagcaggc aatcaatttg actggcgagc ataaaccgtc aatcccagcc cttaccatgg
     8101 cggctctgat ctgtatcgcg caaaatgaca gccagcagtt acttgatgaa atcgtgcaac
     8161 aagaggggct ggaatatgcg acggatgtag tgatagcgcg tcagtgcatt gcgcgacgtt
     8221 atgagagtga ctcactggtc gtcactttgc agtatcagga cgaggactat ggctacggtt
     8281 atggctccgc gacctataac gatttcgatc tccgtttgcg taagcatctg tcgctggcag
     8341 aggaaagttg ctggcagcgt tgtgccgata aactcattgc cgcactgcca ggaataccca
     8401 aaattcgccg tccttttatt gcgctgatcc tcccggaaaa acctgaaata gccaatgaac
     8461 tcgccagtct tgaaagctct cggtcaagtc tccattcaaa ggagtggtta aaggtcgttg
     8521 ctactgataa tacggcggta aaaaaactcg aacgatactg gggcctggac gtatttagcg
     8581 atcgcgaagc cagctacatg tcacaagaaa accgcttcgg ctacgcggcc tgcgcctccc
     8641 ttttgcgcga acaaggactg gcagccgttc cgcgcctggc aatgtatgcc cataaagaag
     8701 attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc ttgctactgg
     8761 tggctgataa aaacaaaccc agcctgcaac gtgtggctaa atacagtaaa aacttccccc
     8821 acgcgacgct cgccgcactg gcagaactgc tggcgttaaa agaaccacca gcccgcccag
     8881 gttatccaat catcgaagac aaaaagctgc ctgcacagca aaaagcacgc gatgaatact
     8941 ggcgtacgct gttacaaacg ctgatggcat cgcagccaca actggcagca gaagtgatgc
     9001 cgtggttaag tactcaagcc agggcagtgc tgaatagtta tttatcggca ccgccaaaac
     9061 cggttattga tagcaccgat aacagcagtc tgcctgaaat gctcgtttca ccaccgtggc
     9121 gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa ttaactccgc
     9181 aaatttactg gcaaccaggc gaacaagaga ggcttgccgc cactgagtct gcccgttatt
     9241 tcagcacgga atctcttgcg gaacgcatgg aacaaaaaag tggacgagtt gtattacagg
     9301 aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc ggaaaactgg
     9361 atgctgcacg caattcactc attgttcagt ggcattacta ccaggggcgg gttgaagaga
     9421 tcctgaatgg ctggaactcc ccgcaagcac aattagcaga acaggcgctc cgcagcggtc
     9481 acatagaagc gttaattaac atatgggaaa atgacaactt ctcacgttat cgtccggaaa
     9541 agagtgtctg gaacctgtat ttattggcac agttgccgcg tgagatggct ttgaccttct
     9601 ggctgcgtat cattgagaaa aagcatctgt tcgcgggtga ggactatttt ctcagtatcc
     9661 tcggattgga tgcgctacca ggtctgctgt tggctttttc acatcgtcca aaagaaacat
     9721 ttccgttaat tttaaatttt ggcgcaacag aactggcgct gcctgttgcc cgcgtctggc
     9781 gacgtttcgc ggcgcagcgt gatctggctc gccagtggat tttacaatgg ccggaacata
     9841 cggctagtgc acttatccct cttgtcttta ccaaacccag cgataatagc gaagccgcat
     9901 tacttgccct gcgtttactg tacgaacagg gacatggcga attgctacaa accgtggcaa
     9961 accgctggca gcgtacagat gtatggtctg ccctggagca gttgcttaaa cagggtccaa
    10021 tggacattta cccggcacgc attccaaaag cccctgattt ctggcatccg gcaatgtggt
    10081 ccaggcctcg acttatcact aataatcagc ctgttaccgg tgacgctctg gaaattatcg
    10141 gcgaaatgct gcgctttacc caggggggac gtttttatag cgggctggaa caactgaaaa
    10201 cgttctgcca gccacaaacg ctggcagctt ttgcctggga tctcttcact gcgtggcaac
    10261 aagctggtgc ccccgcaaaa gacaactggg catttctggc gttaagtctc tttggtgacg
    10321 aaagcacggc acgggatctg acgacacaga tcctcgcctg gccacaagaa ggcaaatctg
    10381 cccgtgctgt cagcggcctg aacatcctta ccctgatgaa taatgatatg gcgctgatac
    10441 agctgcatca tatatcgcaa cgggctaaat cccgcccctt acgtgataac gcggcggaat
    10501 ttcttcaggt agtcgcagaa aatcgcgggc taagccagga agagctagcg gacagattag
    10561 tcccaaccct gggccttgat gatccgcagg cgttgagttt tgattttggt ccccggcagt
    10621 ttaccgttcg cttcgatgaa aacctcaacc cggttatctt taatcagcaa aacgttcgcc
    10681 agaaaagcgt tccccggttg cgcgccgatg acgatcaact gaaagcgccc gaggcactgg
    10741 cccgactaaa agggctaaaa aaagatgcta ctcaggtgag caaaaacctg ctcccgcgtc
    10801 ttgaagctgc cctacgtacc acccgacgct ggtcgctggc agattttcat tctctgtttg
    10861 ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggggtt tatccggcaa
    10921 atgaaccgcg ttgtttactc aaagcctttc gtgtggccgc agagggggag ttctgcaatg
    10981 cgcaagatga gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag
    11041 aaatgacagt agaaatgcgc agtgaatttg cacagctttt tgccgattac gaaattatgc
    11101 cgccttttcg ccagttgtcg cgccgcacgg tgctgctcac acctgacgag tcaaccagta
    11161 acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcagcttatg ggaatgcgat
    11221 acaaaggctg ggagtcaggt tatgaggacg catttgtcta tgacctgggt gagtaccggc
    11281 tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa gcgctaatga
    11341 gcttccgttc tcttcgagtg caccgtgaca ataaatccgt cacttttgcc gaacttgatg
    11401 tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa aaacacagcg
    11461 ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc gatgatcgtg
    11521 caaaaagagc tggttgctat ttacgattat gagatccctg tacctgaaaa tccgttttcc
    11581 ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta tcgactggag
    11641 cgattccggc tacgtccaac atttcatcaa cgtgatcgag aagatactga cccgctaata
    11701 aatgatgcgt tgatttatat aagagatgag tgtattgatg agcggaaatt acgaggtgaa
    11761 tcacctgaaa ctgtaatagc aatttttaat cgtgaactac agaatatatt caaccaagaa
    11821 atagaataat atactctaga taattaaagt cgcagcacat gcaacttgaa gtatgacgag
    11881 tatagctttc ttaaaggggg aaacggtatt cccccttaga tgcgtttcaa tttgcccgtt
    11941 acttcaacaa atcaatcaac cgtaacagag ccactaataa ctccagtacg gcaatgagca
    12001 cggttaactt ttgtgtcagc gtgtccatcc gctctcctta ttaaggagcg caacaccagc
    12061 accttaccct tacactgcct gttgatactg gtttacgtat gttggtacga aacgttgctc
    12121 tccggatcag ggcaccgcct gcggcaccac ccgtccggca gccaggactt aaaattggtc
    12181 cgatccggca caacctccca acgggcagga ttataaatct cactcactga attgcaatgg
    12241 agtgtggata tggttataat ttattcgttg gtacgaaatg ttgcacaatc agcaagttag
    12301 cgtcactgta gtaaataaaa gaacctgcct caccagcagg tttttttatt ttctgcgatc
    12361 tactttccag atattttttg ccaatagccc aacataaata ttcacatccc ggtcaaatgg
    12421 tgcgttgaat ataaaatcga tgaatttata aataaaaatg agttatatga tgaattaata
    12481 aaaacagtaa tcattttatt aaagg
//
LOCUS       DAZKGS010000129        12494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKGS010000129 DAZKGS010000000
VERSION     DAZKGS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(182..1606)
                     /gene="patD"
                     /locus_tag="ACHOQY_003615"
     CDS             complement(182..1606)
                     /gene="patD"
                     /locus_tag="ACHOQY_003615"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9679646.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1628..2422)
                     /gene="ydcV"
                     /locus_tag="ACHOQY_003616"
     CDS             complement(1628..2422)
                     /gene="ydcV"
                     /locus_tag="ACHOQY_003616"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9679647.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2412..3353)
                     /gene="ydcU"
                     /locus_tag="ACHOQY_003617"
     CDS             complement(2412..3353)
                     /gene="ydcU"
                     /locus_tag="ACHOQY_003617"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9679648.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3354..4367)
                     /gene="ydcT"
                     /locus_tag="ACHOQY_003618"
     CDS             complement(3354..4367)
                     /gene="ydcT"
                     /locus_tag="ACHOQY_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9679649.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4385..5530)
                     /gene="ydcS"
                     /locus_tag="ACHOQY_003619"
     CDS             complement(4385..5530)
                     /gene="ydcS"
                     /locus_tag="ACHOQY_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9679650.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5775..7180)
                     /gene="ydcR"
                     /locus_tag="ACHOQY_003620"
                     /pseudo
     CDS             complement(5775..7180)
                     /gene="ydcR"
                     /locus_tag="ACHOQY_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7259..7675)
                     /gene="hicB"
                     /locus_tag="ACHOQY_003621"
     CDS             complement(7259..7675)
                     /gene="hicB"
                     /locus_tag="ACHOQY_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP9679651.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7721..7897)
                     /gene="hicA"
                     /locus_tag="ACHOQY_003622"
     CDS             complement(7721..7897)
                     /gene="hicA"
                     /locus_tag="ACHOQY_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP9679652.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8119..8349
                     /gene="yncJ"
                     /locus_tag="ACHOQY_003623"
     CDS             8119..8349
                     /gene="yncJ"
                     /locus_tag="ACHOQY_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP9679653.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8441..10402)
                     /gene="rlhA"
                     /locus_tag="ACHOQY_003624"
     CDS             complement(8441..10402)
                     /gene="rlhA"
                     /locus_tag="ACHOQY_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9679654.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10475..11011)
                     /gene="sutR"
                     /locus_tag="ACHOQY_003625"
     CDS             complement(10475..11011)
                     /gene="sutR"
                     /locus_tag="ACHOQY_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9679655.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11064..12278
                     /gene="ydcO"
                     /locus_tag="ACHOQY_003626"
     CDS             11064..12278
                     /gene="ydcO"
                     /locus_tag="ACHOQY_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9679656.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12318..>12494)
                     /locus_tag="ACHOQY_003627"
     CDS             complement(12318..>12494)
                     /locus_tag="ACHOQY_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9679657.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3060 a         3352 c         3059 g         3023 t
ORIGIN      
        1 gtttaataac gatactctaa agccttatgc taatcaactg tgacaggatg aaaaatcgcc
       61 atcaaacatt tagcctaatt tttacgctag ttattaatgt aatcattcta atacttaacc
      121 gccattaaac tcagaaccta aaacgatagt ggccgtccta taacagccac taacatctaa
      181 cttaatgttt aaccatgacg tggcggacga cggtgtaatc ctccagccca taaagtgaca
      241 tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg catttcactt accagcatga
      301 aatgggtatt gacccaggta caaccatatt gcagccgtgc gctgacgcga tgcgccctgc
      361 ccacatcttt cgtccatacc gaagatgcaa gtccgtactg gctgtcattc gcccagttca
      421 ccacctgttc ttcgttgtcg aagggcgtaa cactcactac tggaccaaat acctcttttt
      481 gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag taatagccat
      541 tacccttgcg cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct ttcgcctctt
      601 ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca agctccgtag
      661 actcgtcatc cggcgcacca gattttaacg ttgccaccgc agcacccagt ttttccacca
      721 gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc cgcagtacaa tcctgtccag
      781 cattgtaata gccaaatgta cgtacacctt cgaccactgc ttcaatatcc gcatcatcaa
      841 aaacaatcac tggcgctttg ccaccaagtt ccatatgagt acgcttaatg gacgacgcgg
      901 tatggctgat gatgtgctcg ccggtggcga tagagcccgt cagcgacacc atccgcactt
      961 tgggatgacc ggtcagcgga tcacccaccg ttttgcctct gccaaacagt acgttaatca
     1021 cgcctgccgg gaagatatct ttcgccagct ctgccaactt caacgcggtc agcggggtaa
     1081 tttctgatgg tttaagcact acgcagttcc ctgccgccag cgccggagcc agtttccacg
     1141 ctgccatcat cagcggataa ttccacggtg cgatagaagc cacgaccccc aacggatcgc
     1201 gacggatcat cgaagtatga ccttcaagat attcacctgc cgccagacca ttcagacagc
     1261 gcgccgcacc cgcgaaaaag cgaaaaacat cgacaatcgc cgggatttca tcattgaacg
     1321 cactatgcag cggtttgcca caattacggg actccagttc ggcaaaaacc tgaccatttt
     1381 cttcgataac atcagccagt ttcagcagac attccgcacg cactttcggc gtggtttgcc
     1441 cccattcggc aaatgctgca tctgccgcgc gcacagcagc atcgacctgc tctgcggatg
     1501 cctcggcaat ctccagtaaa acgtcccccg ttgccggatt atagacaggc tgtttttccc
     1561 cttcgccgct aaccagttct ccgttaatca gtaacttatg ttgcatagca tattcctgta
     1621 tcagtggtta ttgaccattg tcgccttcgc gggttagcca ccaggccccc aggatcggca
     1681 aggttgttac caacataacc agcagtgcca ccacgttagt taccggtaca tcacgcggtc
     1741 gcccaagctg attgagcaac cacaacggta acgttcgttc atgacctgcc gtaaaagtcg
     1801 taacgatgat ttcatcgaac gacaaggcaa acgccagcat tcctcctgcc agtagcgccg
     1861 aactgagatt cggcaacact acgtagcgga aggtttgcca gccattggcc ccaagatcca
     1921 ttgacgcctc aaccagactc cacgaggtgc ggcgaaaacg ggcgatgaca ttgttaaaca
     1981 ccaccactac gcaaaaagtc gcatgaccga ccacgatggt gaaaaatccc ggctccagat
     2041 tgatggtttt aaaagcggtt aatagcgcca gaccagtgac aatgcccggc agcgcaatgg
     2101 gcagcagcag taacagcgaa atggcgtttt tgccgaaaaa gtctcgtcgc cacagcgcgg
     2161 cagctgccag cgtccctaac accagcgcca ttaatgtcgc cagcgccgcc actttaagtg
     2221 acagtgtcac ggcatcaaga atatcactac gctgtgctgc cacgctaaac cagcgcagcg
     2281 tcaggccctg cggtggaaaa ctaaacgccg catcttcagt gttaaacgca taagcggcga
     2341 taatcaggat gggaaaatgt aggaaaacca cgccgcccca ggccgccagt ttgaggaaaa
     2401 acggtgcgcg ttcagagtgc atcgaacgct cccagacgtt tcacgaacgc caggtacagt
     2461 gcgatgagaa caatcggcac cagggtgaat gccgccgcca tcggcatatt gccaatcgcc
     2521 ccctgctggg aataaaccat attgccgata aaatatcctg gaggaccaac cagctgcggg
     2581 acgataaaat cgcccagtgt gagtgagaag gtaaagatag agccagcggc aatacccggg
     2641 attgccagcg gcagcaccac atagcgaaag gtttgtcgtg gacgtgcgcc gagatcagcc
     2701 gacgcctgca acaatgacgg cggcaaacgc tcaagcgccg cctgaacggg caggatcatg
     2761 aacggcaacc agatatagag aaacaccaga aagcgcccca gcccggaagt tgacagcgta
     2821 tttccgccca ccgcaggtaa tgtaaggaac gcagtcagca gtggttccag cccaagatgt
     2881 tgtaaaaacc actgagccac gccatctttt gccagcaata acgtccaggc ataggcttta
     2941 acaatgtagc tcgcccacat cggcaacatt accgcaatat aaaaaaaagc tttcattttc
     3001 ccgctggtat agcgcgccat ataccacgcc attggaaaag ccagaatggc gctggcgata
     3061 gtgactgcca cagccatagt cagcgtgcgg agaatgatgt cgtaattcgc cggattaaac
     3121 agcgcacgga tattcgccag cgtcagttcc ggcgttaccg acatggtgaa atcgtcaaaa
     3181 gtataaaatc cctgccataa cagtgtcagc agcgagccga aatagacaat gccaaaccac
     3241 attagcgggc caagcagcag taaaaacagc cccagcctcg gattacgcca gaagaaaccg
     3301 gacaccttac ccagacctgg acgtgaaggt gattgcaata cattcatcgc cattcacctc
     3361 tcctcaacca gcggcaccat cacatcacgc gaccaggaaa ccatcacctg ttgtccgggc
     3421 gtgagcgtgg caggcagttc ttcgcctgtc atattggcct gactcacaag cagtttttca
     3481 ccgccgttca atttcagttc aaaacgagtt gccgcgccct gatattgcac cgcctggatc
     3541 gtgccattgg cctgcagttc accaggggtg ttgaggcgga tatgttccgg tcgcagggcg
     3601 aagcttcccg tcatgccaca aagtttctct gccatcagtc catcaaaaac gttggatgtc
     3661 ccgacgaatc cggcaacaaa cggcgtgcgc gggcgcatat agagatcgcg cggggaatcg
     3721 acctgctcaa tgcgtccatt attgaaaacc gccacacgat cggacatcga taacgcttcg
     3781 ccctgatcgt gggtaacgaa gataaaagtg ataccgagag actgttgcag ttttttcagt
     3841 tccagctgca tctgctcacg caatttgaga tccagtgcgc cgagcggttc atccaacagc
     3901 aatacgcgcg gttcattcac caatgctctg gcgatagcaa cccgctggcg ctgaccacca
     3961 gaaagttgtg acggtttacg ttgatgtaca aaccccaacg ccactttctc cagcgcctct
     4021 tgcgccattg cgtgccgctg ctttttattc acgcctttga ccatcagccc ataggcgaca
     4081 ttgtcaagaa tcgacatatg cggaaatagc gcgtagtcct gaaagacagt attcacgtcc
     4141 cgctcccacg gtggcagatt gctggcgggt ttaccaaaga tagagatagc cccgccggaa
     4201 agctgttcga agccagcaat caggcgcagg caggtggttt tgccggagcc ggacggcccc
     4261 agcatagaga agaactcacc atcttttatc gcaatactga cgccatctac tgcgcgcacg
     4321 tcaccgtaca accgcgagac gttgtcaaac tccactgcgt acgtcataaa gcaccccagc
     4381 gaagttagcg accgcccata atggcaatgt aatcctgcgt ccagcgactg tagggaacaa
     4441 acttgccccc ttctgctata ggcgttttcc agaaggcgat tttgtcgaaa tagttaaaac
     4501 cgttggtttc acaacctttt tcgcctaata acggactggc tttacaccct tccggcacta
     4561 ccggtaacga gccaaaccag gccgccacat cgccctgcac ttttggcgtt aatgaccagt
     4621 tcatccattt gtaggcgcaa accggatgtt tcgcttcgct atgcagcatg gtggtatcag
     4681 cccaaccggt aacaccctcc ttcgggaaaa cggtagcaac aggctggcct tcggctttca
     4741 gggcgttggc ctgatagggc caggcactgg aagcaaccac accctcgttt ttgaaatcgc
     4801 tcatttgcac ggtagtgtca tgccagtagc gatggatcaa actgtgttga gcgcgcagca
     4861 ctttcagcac cgcctggtac tgttcttcgg ttagttgata cggatcgctg atgcccaact
     4921 gcggctgagt ggctttaacg aacaacgcag cgtccgcaat gtagataggg ccatcataag
     4981 cctgaacgcg gcctttattg ctcttgccgt ccggcagatt ttgctcaaca aaaaccactt
     5041 gccagctatc cggcggcgtc gggaaggttt tagtgttgta catcagcagg ttcggccccc
     5101 attggtaagg tgtgccgtaa actttgccgc caacattaaa ccagtcgcct ttaaccacgc
     5161 gcggatcgag cgttttccag ttgggaatca aggcggtatt aatcggctgc acgcgtttgc
     5221 ccataatcaa acgcagcgag gcatcgccgg atgccgtaac cagatcgtaa ccccctttgg
     5281 tcatcagact gaccatttca tcggaagtcg cggcggtttt cacattcacc gcgcagcctg
     5341 tctctttttc aaattgcgtt acccagtcgt attgtttatc agtttgtccg cgttcgatgt
     5401 atcccggcca ggcgataata tccagtcgcc cttccggttt atctaaattg gtaggtggtt
     5461 cggcggcgtg agcggtcatt attgtcatgg tgagcgcaca caggctgctg cgggcaaatg
     5521 tcttgctcat aaggtcttac tcctgtcgta attaattgtt cagccgccgt gccgtaaaaa
     5581 tatctccttg tttaaccata gacggcggct tgcaggcaca ccttcgcccc tgagaaaatt
     5641 taatcaggtt gtgagctacc gcgcattatg ccgtctgtaa ggttatcttc tggagtatag
     5701 acaaggggtt aggaaatatg actgctattc gaaattcata ttacccaatc taagaggaat
     5761 taaattaaac gctattacag ccgttcttga ataagtttgc ctaattgttt taccgcctgt
     5821 tcttcacgtt ctccccactg ccacgcggta ttaaaacgaa aaaaacgtga ccagttttca
     5881 ccggtagaaa acattttacc cggcgcaata ctgatatgat gcgtcagtgc cgccaggctt
     5941 aattcgccgg catctaacgg ctcggggagc tccagccaga gaaaataacc gctgtcacta
     6001 tgatgaattt taacttccgc aggcagataa cgcagcagtg cctgccaggc acgttgttta
     6061 cgttccgcaa gctggcgacg caggcgacga agatgggcgt cgtatcggcg cgtggaaagg
     6121 taatccacca gcgcaagctg catcggtgag ctggtggaaa gcgtactcat caactgcaag
     6181 cgttgaattt tacgtgcatg ttttccggcg gcgacccaac caatacgaaa accaggcacc
     6241 agacatttcg aaaacgaaga gcaatgcaaa acgccatcgt ggcgatccca cgctttcgca
     6301 ggcagcggtt tttcccgtcc aaaataaagt tcgctgtaaa cgtcatcttc aatcagcgtt
     6361 acgttgtact gattgagtaa cgccaccagt tgtgcttttt ttgcggcgtt aaggtaaatc
     6421 cgagtggatt ctggctatta gtcatcagcc agcacgcttt caccggatac tcctgcaacg
     6481 ccagttccaa cgcctgaaga tctatccctt ctttaacatc cgtcgccacc gataacgctt
     6541 tcagccgtag ccgttccagc gcctgcaacg cgccatagaa acaaggattc tctactatca
     6601 cccaatcgcc cggttcagtt accgcttgca aactgaggtt taatgcctct aacgccccgg
     6661 cagtaatgac aatttcatca ggagaaatgg tgatgccctg taaggcatag cgacgagcaa
     6721 tagcctgacg cagttctgcg tttcctggcg gtaagttttc aatcacgctc atcgccgtgg
     6781 cggttttgct tacctgcgcc agcgagcggt ttagttgttg gagggggaaa agtcgcgggt
     6841 cgggaaaggc cgaggcaaac ggaacgaccg acggatcgcg actggcctgc aacatatcaa
     6901 aaatataagt gttgatatcg actgcttcat ctcgagtgac tggaatgact ggcgctttcg
     6961 gcatttttat tgcctgtggc gcaacgtaat aacccgactg cggtcgtgcg ataatatatc
     7021 cctgactttc gagcaactga taggcatggc tgacagtcat aaagctcatg cctgaaagcg
     7081 ccacctggtc acgcaacgaa ggcaaacgat cgccgggttg ccagataccc gacgcaatct
     7141 gctcgcgtaa ttgttctgca agctgctggt attttttcat tatcgaatcg taattatgtg
     7201 cagatgattc ggcagtctat atcagttgtt aaaaatgaca acttttcgtt aactgtaatt
     7261 aaaccatcac cagcgataac tctttgccaa gcgcctttgc cgcaagctgg acggcgtcga
     7321 tttttgtcgc atggtgcaag ttaaataggc gagtaatctc ctgtttaggt ttgccaattc
     7381 gcctggctaa ctcttgctga gtgatttctg actgtaaaaa agcatttaac agcaatactt
     7441 tagaggcgac gctcaaaggt acttcaataa agtgatcatg actatttaat ggcgaaggta
     7501 acgggataag ctcgttatct tcaaaataaa aatcaaatgc ggtcagtaaa gcatcttttg
     7561 ccgcttccat cgcttcagcg acagtttcgc cctgcgtcaa cgcttcaggg atatccacaa
     7621 aagaaaccat ataaccgcct tccggcgcgg gtgtaagagt gacgggataa cgcataattt
     7681 cgactgtccc tcgcatgtca ttaacctttt taattggcga ttaactcaaa ccgagttgtt
     7741 tcaggattgc tttacgcaat ggttctttaa tctcatcgca ggggtgacgc ggcatgacac
     7801 tgcgcctccc atgaaacctg agtttcaaat ggttgctgcc attcgctaca tcgacgccct
     7861 gagattcgag ccaacgtctg aactcgcttt gtttcacatc gcctccctgc cgctgagtat
     7921 atcaacaaag taaacattag tgtttacctc cgtgtgtaat ttgctcgaaa atagcactaa
     7981 atcgccgcaa cggcagaatg ccatgcaaaa tcaggaagaa agactccaga atttctttgc
     8041 gctaatttgc aaaactgaat aataacctca agaaacgatc ggcatcatag cggcaaaagg
     8101 taagaaaaag gtggcaggat gtttacgaag gcgttatcgg ttgtcttatt aacgtgtgcg
     8161 ctgttctcag gacaactcat ggcagggcac aaaggacatg aatttgtgtg ggtaaagaat
     8221 gtggatcatc agctgcgtca tgaagcggac agcgatgaat tgcgtgctgt ggcggaagag
     8281 tcggcggaag gtttgcgcga gcatttttac tggcaaaaat cgcgcaaacc agaagcggga
     8341 caacgttgag tcatttgttg ccggatgcgg cgagacgcct tacccggcag gcaatatcat
     8401 gcagattcaa tgagttgtga gtaaccagaa aactggcgtt ttaccctttt cgcttaggca
     8461 atgttttcag cagctcatcc ggacttacgg atgccactac gcttcccggt aacggcattt
     8521 tcagtatgtg atttttgatt ttgccaatga cgtgcatctc gcatgggcgg caatcaaact
     8581 ttagcgttaa tacttcatcg ccgttaacca gttgcattgg cgtcgccttc cagcttttga
     8641 tattgccttt cgcctgtttc gggcacagat taaaggcaaa gcgcagacaa tgcttggtga
     8701 tcatcaccgg gacttcgccc ttctcttcat gtgcttcata cgccgcgtca atcagctgca
     8761 caccatagcg atgataaaat tcacgcgctt tctggttgta tacgttcgcg aagaaactca
     8821 gatgcgtttg cggataaacc ggcgcaggat cagcaaccgg tttacggctg ccgcgctggt
     8881 aactggcaag acgcgcagca tccagcatgt cagcagcttc acggcggaac tggtttaaca
     8941 gactgtttgg tacaaacagc gcccccggca aattaatttg cacatcgcgg gcgtaataga
     9001 gggtttgccc cagttttgcc agaccatcct tcagattgtt cattgctttt tcggcgttat
     9061 tggcttcgtc gaactgcccg tccagcgtat gcgtgatgct gacaccctct tcactggtga
     9121 gggtcagaat cagttgttcc tgccagccgc ccagttcaat gtctaccgcc acccgacgtt
     9181 cgctggaggt ttttgtcagt gcctgctgcc agttatgatc aagattacgg tttagtgggt
     9241 gatgtggacg aattttgtgc aaatctgctg gcatttcatt gggccagacg cggtactgat
     9301 tttctccggt tttctcgacc gtattggcac gaaaaccgac gacttcacgt ttaatcatca
     9361 cgttcaggcc atcaccattt gccagtggct cggtgacggc aacatcgaga tgatctttcg
     9421 ccactttcaa tacttcgcct accggcaggc cgataaattt cggcgaatcg aacgcgccaa
     9481 tatcgccttt acgggcattc acaaaataat ctgtgctacc acggtggaaa gtcttttccg
     9541 tcgatggaac aaagaaatgt tcagtacgac ctgatgaagc gcgcgccaga tcgccacgtt
     9601 cttcaataat ggcatcaagc atctggcgat aatgggcggt gatattcttc acgtagctca
     9661 tatctttgta acgcccttca atcttgaagg agcgtacgcc agcatcaatc agcgcgccga
     9721 ggttggcagt ctgatcgtta tctttcatcg acagcagatg tttttcatag gaaaccaccc
     9781 gcccctgatc gtctttcaat gtgtatggca aacggcacgc ctgcgagcaa tcaccacggt
     9841 tggcgctacg ccctgtttgc gcatgagaaa tgtagcactg acccgaatag gccacgcaca
     9901 gtgccccatg aataaagaat tcaatggtcg cgtccgtggc ctggtgaatc gcgcggatct
     9961 gctcaagatt cagctctcgc gccagcacaa tctgcgtgaa gccaacatca gagaggaact
    10021 tcgctttttc tactgtacga atgtcgcact gcgtactggc gtgcagttca atcggcggaa
    10081 tatcaagttc cagaatcccc atatcctgaa caatcagcgc atcgacaccg gtctggtaga
    10141 ggtcggtaat cagccgttgc gcgggttcca gctcatcatc atgcaaaatg gtgttaagcg
    10201 tgacgaaaat ttttgcacca taacgatggg caaacggcac cagctcggca atatctttca
    10261 ggctattact ggcattatga cgggcaccaa aaccagggcc gccgatataa acagcatcgg
    10321 caccgtgcaa aatagcttcg cgggcaatgg cggcatcgcg tgccgggctt aacagttcaa
    10381 gtcgatgaga agatacggtc acattcttat ccagtagctg aaaaatggcg gctattttag
    10441 ccacaagcgc gacgttgcga aatagttttc ccgcttaact gcgcgggtaa tggatgaggg
    10501 aatgaaaatg tacggtttgc tccccaccat tacgatagat atgcgtgacg tctgcagcaa
    10561 atcgaacgcc ttccccacaa ttaagggtct gccattcccc atcaacgcac agatcaagtt
    10621 gtccatcaat gacgacgaca tgttcgatta cccccttctc atggggcgtc gattcgctta
    10681 ttgcaccaga tgccatctga atcgagaagt gttcgaagca gagttgtgga tcataaggaa
    10741 acagcgaagt gatcaccatc gcctgctgtt gtggatcata aactgaaggc gtagcggact
    10801 gcggcggaga aataaatgtt gaaaatggta cgttcaaccc ggtggcaatt ttccataatg
    10861 tcgcgaccgt cggactggac tcattacgct caatctgccc taacattgct ttagaaacgc
    10921 ccgtcgcttc tgccagtcgc gaaagactcc agccgcgctg ctggcgtagt tgtttaagtg
    10981 tggtggataa aaagcgagcg agattttcca tgcgactcct ccggcaaaac ggaagtttat
    11041 cacttgtgcg ttataacgga caaatgctac ggtgcctgta cgctataacg cacgaggtga
    11101 ctatgcgtct gttttctatt cctccaccca cgctactggc ggggtttctg gcggtattaa
    11161 ttggctacgc cagttcagcg gcaataatct ggcaagcagc gattgtcgcc ggagccacca
    11221 ctgcacaaat ctctggctgg atgacggcgc tggggctggc aatgggcgtc agtacgctga
    11281 ctctgacatt atggtatcgc gtacctgttc tcaccgcatg gtcaacgcct ggcgcggctt
    11341 tgttggtcac cggattgcag ggactaacac ttaacgaagc catcggcgtt tttattgtca
    11401 ccaacgcgtt aatagtcctc tgcggcataa cgggactctt tgctcgtctg atgcgcatta
    11461 ttccgcactc gcttgcggcg gcaatgcttg ccgggatttt attacgcttt ggtttacagg
    11521 cgtttgccag tctggacggt caatttacgt tgtgtggaag tatgttgctg gtatggctgg
    11581 caaccaaggc cgttgcgccg cgctatgcgg taattgccgc gatgattatt gggatcgtga
    11641 tcgttatcgc gcaaggtgac gttgtcacaa ctgatgttgt ctttaaaccc gttctcccca
    11701 cttatattac ccctgatttt tcgtttgctc acagcctgag cgttgcactc cccctttttc
    11761 tggtgacgat ggcatcgcaa aacgcaccgg gtatcgcagc aatgaaagca gccgggtatt
    11821 cggctcctgt ttcgccatta attgtattta ctggattgct ggcactggtt ttttcccctt
    11881 tcggcgttta ttccgtcggt attgcggcaa tcaccgcggc tatttgccaa agcccggaag
    11941 cgcatccgga taaagatcaa cgttggctgg cagctgccgt tgcaggcatt ttttatttga
    12001 tcgcaggtct gtttggtagt gctattaccg ggatgatggc tgccctgccc gtaagttgga
    12061 tccagatgct ggcaggtctg gcgctgttaa gtaccattag cggcagtttg tatcaggcgc
    12121 tgcataatga gcgtgagcgc gacgcggcgg tggtggcatt tctggtaacg gcaagtggat
    12181 tgacgctggt cgggattggt tctgcgttct ggggattaat tgccggaggc gtttgttacg
    12241 tggtgttgaa tttaatcgct gacagaaacc gatattgaca tcctccacgc cctgaaggac
    12301 gaggatccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac
    12361 gggcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg
    12421 ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta
    12481 ctttgtgaca ggcg
//
LOCUS       DAZKGS010000130        12465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKGS010000130 DAZKGS010000000
VERSION     DAZKGS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            142..972
                     /gene="yjiC"
                     /locus_tag="ACHOQY_003628"
     CDS             142..972
                     /gene="yjiC"
                     /locus_tag="ACHOQY_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9679658.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1113..1886)
                     /gene="uxuR"
                     /locus_tag="ACHOQY_003629"
     CDS             complement(1113..1886)
                     /gene="uxuR"
                     /locus_tag="ACHOQY_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9679659.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2101..3561)
                     /gene="uxuB"
                     /locus_tag="ACHOQY_003630"
     CDS             complement(2101..3561)
                     /gene="uxuB"
                     /locus_tag="ACHOQY_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9679660.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3642..4826)
                     /gene="uxuA"
                     /locus_tag="ACHOQY_003631"
     CDS             complement(3642..4826)
                     /gene="uxuA"
                     /locus_tag="ACHOQY_003631"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9679661.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5278..5370
                     /locus_tag="ACHOQY_003632"
                     /pseudo
     CDS             5278..5370
                     /locus_tag="ACHOQY_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5613..6515)
                     /gene="fimH"
                     /locus_tag="ACHOQY_003633"
     CDS             complement(5613..6515)
                     /gene="fimH"
                     /locus_tag="ACHOQY_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9679662.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6535..7038)
                     /gene="fimG"
                     /locus_tag="ACHOQY_003634"
     CDS             complement(6535..7038)
                     /gene="fimG"
                     /locus_tag="ACHOQY_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFP9679663.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7051..7581)
                     /gene="fimF"
                     /locus_tag="ACHOQY_003635"
     CDS             complement(7051..7581)
                     /gene="fimF"
                     /locus_tag="ACHOQY_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9679664.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7591..10227)
                     /gene="fimD"
                     /locus_tag="ACHOQY_003636"
     CDS             complement(7591..10227)
                     /gene="fimD"
                     /locus_tag="ACHOQY_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9679665.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10294..11019)
                     /gene="fimC"
                     /locus_tag="ACHOQY_003637"
     CDS             complement(10294..11019)
                     /gene="fimC"
                     /locus_tag="ACHOQY_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9679666.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11056..11595)
                     /gene="fimI"
                     /locus_tag="ACHOQY_003638"
     CDS             complement(11056..11595)
                     /gene="fimI"
                     /locus_tag="ACHOQY_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9679667.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11660..12208)
                     /gene="fimA"
                     /locus_tag="ACHOQY_003639"
     CDS             complement(11660..12208)
                     /gene="fimA"
                     /locus_tag="ACHOQY_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9679668.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3097 a         3231 c         2945 g         3192 t
ORIGIN      
        1 tcctaccctt taatatcacc gtgtagagtt gatatcacta caacagaaac atgcatgtca
       61 tttgtttact tctcatttta ctacagaaaa atttatctat ttttctaaac atcagtgaaa
      121 tactcataaa aggtaactat tatgtctatg ccattaagca atacccttca gagccaaatt
      181 attacagata atcacttcct tcatcatcca aaagtcgaca gtgagttaac acgtaaatat
      241 gaacgtgcac gcctggatac ggaaaatatc tatcttctgc ctcttgctcg tggcaataac
      301 cataattacg atggaaaaag cgttgtcgaa attagaaaac ttgatattag taaagagtcc
      361 tggccattta attatgtgac agaagcatgc agagaatccg atggtattac aacaacagga
      421 cgtatgctct atcgaaactt gaaaatcacc tcagctttag acgaaatata tggtggcata
      481 tgtaaaaagg cgcatgctgc tacagaatta gcagaaggtt tacggctaaa tttatttatg
      541 aagagccctt tcgatcctgt tgagaactat acagttcatg aaataacatt aggccctggt
      601 tgcaatgttc caggttacgc cggaaccacg ataggatata tttcaaccct tccagtttct
      661 caggcaaaaa gatggaccaa tgagcagcca agaattgata tctatattga tcagataata
      721 actgtctcag gggtagcaaa ttcttccgga ttcgcccttg ccgcattact taatgctaat
      781 attgagatgg gaaatgaccc aatcattggt atagaagcct atcctggtac cgcggagatc
      841 catgcaaaaa tgggatataa agttatcccc ggtgatgaag atgcgccact caaacgcatg
      901 actctgcaac cgtcatctct tccagaactg tttgaattaa aaaatggtga atggaattat
      961 attggtaaat agccactgag aagaatgagt actaatattc aggttataca gatatttaaa
     1021 caataccctg caatttacaa agcaatactc attgcgactt cttaatttaa gaaagcgcaa
     1081 ggaacgttta cccttgcgct tattataata agtcagacgt cgactttatc cagcggccat
     1141 gaatcaaaca gatagccgtc aaaataaata tcgtcaacgt tcgagaattc cagcagacgt
     1201 tgcttaacgt tttccagatg ctgccacatt gccagcttcg ccgctcgggc atcttttttg
     1261 attaacgcgg cgaggatctg tttgtgatcg cccaaccact ctttgcgata caggctgtca
     1321 tccagatggc tgtgcaactg gatccacatt ggattgtttt cccgccactg ccaggactga
     1381 cggaataact ccaccagcat gctgttatgc gttgcttcgg caatagcgag atggaactgc
     1441 atgtcaccgc tttcgctgct gcccggcgca ctggaagcca gttcacgctc ttccagttgc
     1501 aatgcctgac gcattttgac gatatcttcg cgggtagcct gcaaagcggc aaactcggcg
     1561 atgttgctct ccaataactg ccgcgcctgt aacagctcaa aaggaccggc atcgttgcag
     1621 acgttggcat ccggactgtc tgtgttctgg ctgcctgagt tatcaagaac atagataccg
     1681 gcaccccggc gtacttccac cagccctttg atctccagca tgatcagcgc ttcacgtacc
     1741 accgtccgcg tgacatcaag catttctgca atttcacgct ccggcggcag ccgttcgcca
     1801 ggattgtacg gcgtctttat gatcagatcg cggatcatcg ccccgacttc ctggtaaggt
     1861 ctttgcgcag aggtggcaga tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt
     1921 tttgccggat gcggcgtgaa cgccttatcc ggcctacaac gggcaatttt acggcccccg
     1981 ttaatctcaa actataccat tagcactgga agttggcacg tacaggtggg caaaaaaacg
     2041 aaaactggtc aaccaaatat cagtttgatt gaccagtaag tctgtagggt aatcagttag
     2101 ttacagcgca gccacacact cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt
     2161 cactgcgcca acaaagtcgg cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc
     2221 cagcagcgct ttcacgcggt ctgcgccctg atactgcgcg ttgaccttct ggaactccgc
     2281 cagcatcggg tcgaccacgt caatggcatt accctgctca tccacgccct gggtgtaacg
     2341 catccagcca gccacgccca gcgccaggtg acgccagctg ccgccgtttt gcaggtgcag
     2401 acgcaccggg tccagcagac gctgcggtaa cttctggctg ccgtccatcg caatctgcca
     2461 ggtacggtga cgcagagacg ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt
     2521 caggtccgta ccttccggca tcgacagcgt tggcgcttgt tcctgcatca tcaaggagaa
     2581 ggccgctttg cgataatccg ggttagtcat ggtgtcggca atggtttcat agccgccgag
     2641 gtaaccgagg tatgccagga aagagtggct gccgttcagc atacgcagct tcatcatttc
     2701 gaacggcaca acgtctgcaa cgaactgtgc gcccacttta tcccagtccg ggcgaccgtt
     2761 aacgaagtta tcttcaatca cccactgacg gaacggttcg caggcaatgg cgcacgggtc
     2821 gtaaacaccc agctggtcag caatttcctg caaggtttct ggcgtcgccg ccggaacgat
     2881 gcggtcaacc atggtgcacg ggaaggtgac gttttcttca atccatgccg ccagctgcgg
     2941 gtcacgcgcc tgagccagtc ccagtaccgc gaccttcgcc acatggccgt tttcacgcac
     3001 gttatcgcag gacatcaccg taaacgcttt caaccctttt tcacgacgca gccgcagggc
     3061 ttcgacgatg taaccaatcg cggacttcgg cgcagtcggg ttttccagat cgtgcttgat
     3121 cagtgggtta ttgagatcca gctgaccgct tgccgcatca gcgcagtagc ctttttccgt
     3181 gactgtcaga gagacaatcg ccgtctgcgg acgcgccatc gcattgagaa taccttcgca
     3241 gccgtcgatt tccggatgca gcgcttcttt catcgaaccg ataattttca gctcggtgct
     3301 ttccgcgcct ttttccgcca cggtgtacag cagttgctgt tttttcaggt tttcgatcag
     3361 cacgcggtcg ttgcctggca tcaggttaac ttcgcagata ccccagtcgc tgtcggtgct
     3421 ttccagcagg tgatgggtat acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag
     3481 atgcacaatg cgtgattcca gacgagaatg ctcccatgac gggcgggcaa ccggcagatt
     3541 gctgtcaaca atagtagtca tcgtaaactc cagaatccgt cttcagagag tgacggcaag
     3601 gaagaaggaa ccggcacggc gcgcagccat gccggtgggt attaacggct aaagaaagcg
     3661 cgctggatcg ccagttcgac accgcgaact tcggccaggc ctttcagacg accaattgcg
     3721 gagtaacctg ggttggtttt cttcttcagg tcgtccagca tctgatgacc gtggtccgga
     3781 cgcatcggga tcaggtcttc tttgccttcc gctttacgac ggtgttcttc ttcaacaatc
     3841 gctttcacca cttcgtacat atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt
     3901 ttcgggttat cttcacgcat ggtggagcgc agatgggtga agtaaatacg cggaccgaac
     3961 tgcttgatca tatcaaccag atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg
     4021 gtgaaaccgt tcgccatgct gtttacggta tcaaccatcc actgcatatc ttcaatggtg
     4081 gaaacaatgc gcggcaggcc aaggatcggg cgcggcggat cgtccgggtg aaccgccata
     4141 cgcacgccaa cttcttcagc aactggaata atcgctttca ggaagacggc aaagttttcg
     4201 cgcagcttcg ctttatcgat atctttgtac agctccaggt gcttacggaa ctggtcgagg
     4261 gtataccctt cttccgcgcc cggcagaccg gcaatgatgt tacgcgtcag acgcgctttg
     4321 tcttcgtcgc tcatagtggc gaagcgaaca gcagcctgag caatttcttc ttcggtgtaa
     4381 tccgcttccg cgcctggacg tttcaggata tgcatttcga atgcagcgaa ttcgatctgg
     4441 tcgaagcgca gagctttgga gccgtctggc agcacgtatt cgaggtcagt acgggtccag
     4501 tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc
     4561 agagtctgct gatagttagc gatccactgc tcatagttgc cagtgtgggt tttgatatct
     4621 tcgtgaattg gtacgctttc tacgacagac cacaccaggc ctgcgtcttc aacgatcgcc
     4681 ttgcgtttga ggatctcttc tacggaccat acttcgccgt tcgggatatg gtgcagcgcg
     4741 gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta aagaaaccgg atcgtttggg
     4801 ccgtaccagc gccaggtctg ttccatgatt tttcctcttt cacatagtgg gttccacagc
     4861 aaagagacgc ggaaccctgg agttgtttag gtatccggat gaacaaccag accgcagccg
     4921 tggcgttgca tcggatgaga tggcgtataa gttctacctt ttggtaagac atgtcaaccc
     4981 gaattctgaa attggttaac cacatcacaa gaatttcact ctcatggttg ttgctgcaaa
     5041 ttgcagcaaa gggtaatcga tggttattga tcagaggatg tgacattcat cgcaacaatg
     5101 gttgaccaat ttacataaca tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa
     5161 acgttatgca tgtgcttaac attctctggg tggtattcgg cattggcctg atgctggtac
     5221 tgacaaagac cgcaggttca gacacattcg ctgcattagc aatgcctctt agataatttt
     5281 gacctgtcag taaaagacac gttgaaaacc tggggtctgc tggagctggt caactccgtg
     5341 gttgggctga ttattagcaa ggtagcgtaa aagaacacca gagcctgcca atggcaggct
     5401 ctgactgatg acaaagtcta aaaatgtgcc cggacagtcc cctcgcccct ccggggagag
     5461 ggttagggtg aggggaacag accagcaaag tgcgctttgt ttatgccaga tgcgacgctg
     5521 acgcgtctta tctggcctac aaagggctaa cgtgcaggtt tttagcttca ggtaatattg
     5581 cgtaactgca ttagcaatgc cctgtgattt ctttattgat aaacaaaagt cacgccaata
     5641 atcgattgca cattccctgc agtcacctgc cctccggtac gtgcgtaatt tgccgttaat
     5701 cccagactta ccgccgaagt ccctactgct cctaacgata ccgtgttatt cgctggaata
     5761 atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg ctggtgaaaa cgacgcggta
     5821 ttggtgaaaa tcgagttgcc cgcatctacg gttgtgccgg agaggtaata ccccagattt
     5881 tggcttttcg cacaataaac ggtaagagga attggcactg aaccagggta gtccggcaga
     5941 gtaacggtga catcacgagc agaaacatcg cagccgccag taggcaccac cacatcatta
     6001 ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt tatagttgtt ggtctgtcgc
     6061 aaaataagca cggcaattaa tgagccagct ttaatcgcca ccccgcccgc actgctcaca
     6121 ggcgtcaaat aaagcgccac cggccacggc ttatccgttc tcgaattata aacaacgcgc
     6181 ggcgtttcgc tggtggtagg aaatggatcg ctactgccac tatattttac ggtcccggaa
     6241 aaattagata acacgctgcc ataagccgag cctcgttgca gtgtgacata gtctgtaatg
     6301 gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa gatccacgac caggttttgc
     6361 cccacattca cggcgggcgc aaggtttaca taaacattgg cgctgccacc gccaataggg
     6421 atagcggtac cattggcggt tttacaggcg aatgaccagg catttaccga ccagcccatc
     6481 agcagtacag caaacagggt aataactcgt ttcattacaa tcatctcttc gggttcaact
     6541 gtaggtatag gtgatgctaa tcactgcctg aatggttccc tgagtggctc cgccatttac
     6601 cgtcaatgct ctgacctgta acgggaagtg cgctgattgt gaggaatcat ccacctgaac
     6661 tgttttggtt gcgccagtat tcaatgtgtt gccactgtca tcctgtagct ctaactggat
     6721 gttttgcgcg gtcccctggt ttttataata tccggtactg tcggctgccc cgctgaagct
     6781 ggcggtgacc ctcgacgttc ccaccggaca attagtcaac tcaagcgcaa catcatgcca
     6841 ggccgatgcc gccccggcag acatcagact gaaagaataa agatcgccga gatcaaccgt
     6901 ggcattggtg gtggaaaccg tacacggttt ggcgacgacc ttaccgttca ccgtgatggt
     6961 gacatcggct gcctgtatcg tcgcacttgc gagcgccaat attgccgcca ataaataccc
     7021 acgtttgcgc catttcatga gcatctccag ttactgatat tcaagagtga aggtagccgt
     7081 ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc attagccggg cgtaaaaatt
     7141 caacgtattt ggtttacccg gcgtcagggt cgtccacgaa atcgcggacg atggagcatt
     7201 aaggggtatt tgattttgct gctcattcag aagctgtatt cccagtcccg aagccgctga
     7261 caccgtattt tcaagtgcaa gcagattggc attgtggcta tctgcaacgc cggtaaaccc
     7321 aacttttacg gcagaaacgg cattaccaca gggtgacagc aaaatacgaa atggaacaac
     7381 aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg ttttccatca gatcaacagt
     7441 aaaattggtt gattcagcgg ccacactaca gccgttatct ctgacatagc cgcggatagt
     7501 aatcgtgcta tccgcagcca aagcgtgact caccgccagc cacaaaaaag cgcacagaag
     7561 ataaaaaggt ttgtttctca tcacgccccc ttaacgacat tcagctgata gctgggttaa
     7621 taactgctgc tgactctctg gtggcagttg ataattggcg acacagtgag cattttcctc
     7681 ttctccccat ttcacctgaa cttttcccgc taaaggcatt ccgctgaggt aaacctgacc
     7741 attatccgca acaatgccgc tactctggct actctctgat gtcaccatcg ccccaaacgg
     7801 cagcggctta ttattgtggg ttagcgtcat gagcagtttt atcccaacgc gcgctttaaa
     7861 ctctgctcgc acgatcgccc cacgagtggg aacaacgtta gcgaccgcgt tatctaaatc
     7921 gacgttatca gccagggtat tggtatccag cgccactctg ttttccctat attcagtggc
     7981 ataaggcagc acggcataac cgcgccagtc ggtacgcacc cccgtctggt tttcgacttt
     8041 tgcatctttt gcgccaggcg ctttaacaag caccaccgta tcgtttaacg gctgccccag
     8101 cgttacgcca ttggcatgag ccaatacccc accgctgact ccgtaataga gctgcttaat
     8161 atcatcgcta tggctgtaac cgatattggc attgccgtaa ccaccgcgat aattcagcgt
     8221 ggcgtagcct gtgcttccgc tattaccatc gcctcccccg gcatagccgg tttgcacgct
     8281 atagctgagg ttgttgtctt ccagcaacgt accgtataca ccagccagat tggtcatccg
     8341 accgttgaga tcgtgtgaca tgctgtagct ggcactggca tgtcgccact gagatttact
     8401 gtcagaacgc agccagtggc tgaaaggaat attgacgtta agcgctaaca tctgatcacg
     8461 tcctttttgc caggcgtttt tcgtcaggct atagctgagc gtccagttga tatcttcgaa
     8521 cgcagtattt aatccagcct ggaattgctc atcgacatta ctcgttcccc aataagtttg
     8581 atggctacca ctcaaataca gtgttgatgt gcgcccgagt tgctgagtaa cggtgagctg
     8641 taatttcccg cgtttgttat aagcgaggtt gtaatagtcg gtgaatttcg gcttaacctg
     8701 aataactcca tcctgtgttt cgatgttgta gccattcatt cgactgtatg ttgtatcagc
     8761 gaaattaaaa tatccgctgg tcgaataacg gtaacccact aactgaatat tcgtgcctga
     8821 ttcattgagc gatttgttat agagaaaacg caccgattgt ccgtcatgct gactgtcatc
     8881 gggaagtgtg gaattagcct gcgtcatatc cacagacaga gcgcccagtg cccccatgtt
     8941 tttcccgata ccgaaattaa aagcacgata acgatccgcc agttgcgttc caccatatat
     9001 tgtccagcca gccggaaggc cgtggagtaa tgtactctgg aaaaagcggg gtttttcctg
     9061 ctgcgcattt ccactacggt attctcctgc cgtaatggaa taacgagtat gcccttcacg
     9121 ttgcaaaagc gggactgacg aatagggtac ggtaaaaatc tgcgtgctgc cgtcagcctc
     9181 tttgatcgtt acctgcaagt caccactatt acctgcggca tagatatcgt tgatggtaaa
     9241 aggccccggt ggcaccgtac tattataaat gtcataccca ttttgtttaa tagtgacctg
     9301 tgcagtacca cgagcaatac cgtggatcac cggggcaaat cctctttggc tatcgggtaa
     9361 catattgtca tctgaggcca attgtgcgcc gcgaaagtta ataccatcga aaatatcgcc
     9421 ctgagtataa ccatcaccca gcgtcagccg ggaacgtaac ggtattatgt ctcgctcaag
     9481 ccaggtattg atatgctgcc atttattttt gctacctgat gatctgtcgc tactgttata
     9541 actccaggtg gtattgtcgc gtaaacgcca cgcaccaata tttaacccac tctgtaggtt
     9601 taaatatgca taatggctgt tacccccaat ccgattctgt acactatttc cgctgaaatt
     9661 ataattgagc aatccggcat taataccggg atcccataac tcaggaggaa tataaccacg
     9721 cgcgcgatta ctcataaatg cctgagggat cgtcaggttc agtcgctgct gaccaacatc
     9781 cagatgcgca gtagcgtcct ggaccattgt ggttaatggc acacaggcat catccgccag
     9841 cagattcata ccggcgacag aagccgtatt cagccccata ctggcgagtt gcgcgcgtgt
     9901 caggcaggga acaatccctt gttcactgtc gcccgtatta aatgtgacat cacgcgttgc
     9961 catataacca ttattcaaat agatatcgac gcgatacgtc cctggcggta attcttgccc
    10021 attttcaaaa cgcgataaat cggccacagc ctggggatca tccgctaaaa aacgcgggtt
    10081 aaaatagagt tcggcagatg acaaaggtgc ctgtgcggca aaagcacagg caacaaagag
    10141 ccggacaaaa aaaccagcca aacgatgctt acgaatatgc aagcattgtg tgtttcgctg
    10201 gtaaagtctt aaattcagat atgacattat tcctccggtt taaagtcacc cggcagtcaa
    10261 ttctttttat tcaggcgaaa aattccccct gcgttattcc attacgcccg tcattttggg
    10321 ggtaagtgca ccataatcat ttattgttcg gtaagtaata ttgcttcccg catcagaagg
    10381 caatttaacc gtgctttcgc ccattggagg caccaaggca ttttcaagaa cccgggttcc
    10441 ggcattcaac tctgttaccg tcaggtaata gggtgtcggg ttaatcagcg tcagagaatt
    10501 cgcgctacga cgaaatctta atttttctgc ggcctgatcg ggtggcaacg ctaatttagc
    10561 cgggcgatag tacagtttaa tgcggctgat aattgcgagc tgtagcgtat tctcagtcaa
    10621 ttttgattta tccattgacg gaatcgcttt aacattcatc cagaataaac tttcccgatc
    10681 ctgtggcaat tggttatttg ttgcatcaag aatacgcaag gtattctctt tttttccctt
    10741 catcgcaaac agaggaggcg tcacgataaa acgaccatcc tttacaccat cggcattttc
    10801 cacccatgat tgaattaaat aggtactatt ttcatcatta tttgtcacgg caagttgcac
    10861 ttgtttttgc cctgccggat aaattacgcg agtcgcacct aaggccactc ccgcttcagc
    10921 gcgtccggca accatcattg ccataaacat caggatacct gccagcaagc agaatgttat
    10981 ttcctgcgat ttccttacat tgacgttttt attactcact gtcctgttcc tgttatcaca
    11041 ttatctgctg aacaattact gataggttaa agagaaccag gcctgggcat tggcgatgcc
    11101 gccagtaacc cgacgcccgg tagcacgata tttggcgatg aaatgtagcg aagtagagcc
    11161 tgagtaaagc cgtttccagt ttgctggagg acgattaatc ggtacgaggt ttccttcatc
    11221 atcaaacaac gctacgccaa tattggtggc tatccctggc ccctctccca cggaaagcac
    11281 atccggattt ttaccatccg cgacaccgtg aaacgccacc cccacacgtt cactcaccac
    11341 cgtgctacat tcccgtaaat gaataacaaa aggcaccggt gcgctatctt cccctgccgc
    11401 atgaaaccgg ttactgctga tttgccccat attgaccgtc atttgtttat caccggcttc
    11461 aatccggcaa gtttccgcaa taatgacgcc ctgaaattgc atatttccgc cgggcagcgt
    11521 ggtattccat ttatttccgg ccagagcaaa cattggcagc aacgacgcta acagaaataa
    11581 tctcttcctt ttcatcgtta tttttatcgc acaagggtgg gcatccctgc ccgtaatgac
    11641 gtccttgaac ctgggtaggt tattgatact gaaccttgaa ggttgcatcc gcattagctg
    11701 cacccggggt tgcctcgccg attgcataat aacgcgcctg gaacggaatg gtgttggtac
    11761 cgttattcag ggttgtttgt gcactaaatg tcgcaccgtc cagcgtcaga gcattgcctg
    11821 ttctgtccag gatctgcacg ccaacgtttg ttgcactacc tgcagcagaa ctctgcagag
    11881 ccagtacatc agtacgcgta gcatcaattg ccgtacctaa gaaggcaaca gcggcttttg
    11941 tggcaacagt ggtatcgcaa tcattcaact gaatgttaaa accaacggca gagctggttg
    12001 ctccagcctg cttcaggcta gcggtacgaa cctgtcccaa ctgaacggtt tgatcaacag
    12061 agcctgcatc aactgcgcaa gcggcgttaa caacttcccc tttaaagtga acggtcccac
    12121 catttaccgt cgtagtatcg gccagagccg ctgcggaact gagggacaga gccgacagaa
    12181 caacgattgc cagagtttta attttcatgc tgctttcctt taaaaaaact atttctaaat
    12241 cgacatgggc agtcgttctg tacactttgt tttgtcaacg agtttgactg ccaacactgc
    12301 acagttttcc cccaaaagat gaaacatttg gggccatttt gactcataga ggaaagcatc
    12361 gcgcacaaac tttttcagtt tatttgttgg cttaatattc tattgttatc tttatttata
    12421 gatttttata ttgcatgagg tggtttttgg agagaagaat gagga
//
LOCUS       DAZKGS010000131        12016 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKGS010000131 DAZKGS010000000
VERSION     DAZKGS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..321
                     /locus_tag="ACHOQY_003640"
                     /pseudo
     CDS             1..321
                     /locus_tag="ACHOQY_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2..66
                     /locus_tag="ACHOQY_003640"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            844..1359
                     /gene="ehxC"
                     /locus_tag="ACHOQY_003641"
                     /gene_synonym="hlyC"
     CDS             844..1359
                     /gene="ehxC"
                     /locus_tag="ACHOQY_003641"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP9679669.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            1361..4357
                     /gene="ehxA"
                     /locus_tag="ACHOQY_003642"
     CDS             1361..4357
                     /gene="ehxA"
                     /locus_tag="ACHOQY_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP9679670.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            4407..6527
                     /gene="ehxB"
                     /locus_tag="ACHOQY_003643"
                     /gene_synonym="hlyB"
     CDS             4407..6527
                     /gene="ehxB"
                     /locus_tag="ACHOQY_003643"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9679671.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6531..7970
                     /gene="ehxD"
                     /locus_tag="ACHOQY_003644"
     CDS             6531..7970
                     /gene="ehxD"
                     /locus_tag="ACHOQY_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP9679672.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            8037..8180
                     /locus_tag="ACHOQY_003645"
                     /pseudo
     CDS             8037..8180
                     /locus_tag="ACHOQY_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8184..8375
                     /locus_tag="ACHOQY_003646"
                     /pseudo
     CDS             8184..8375
                     /locus_tag="ACHOQY_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(8383..8736)
                     /gene="cmi"
                     /locus_tag="ACHOQY_003647"
     CDS             complement(8383..8736)
                     /gene="cmi"
                     /locus_tag="ACHOQY_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFP9679673.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(8909..9691)
                     /locus_tag="ACHOQY_003648"
     CDS             complement(8909..9691)
                     /locus_tag="ACHOQY_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9679674.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(9693..10106)
                     /locus_tag="ACHOQY_003649"
     CDS             complement(9693..10106)
                     /locus_tag="ACHOQY_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679675.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            10666..10896
                     /gene="vapB"
                     /locus_tag="ACHOQY_003650"
     CDS             10666..10896
                     /gene="vapB"
                     /locus_tag="ACHOQY_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFP9679676.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            10893..11309
                     /locus_tag="ACHOQY_003651"
     CDS             10893..11309
                     /locus_tag="ACHOQY_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP9679677.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDKFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(11471..>12016)
                     /locus_tag="ACHOQY_003652"
     CDS             complement(11471..>12016)
                     /locus_tag="ACHOQY_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679678.1"
                     /translation="MQICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFH
                     RRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNL
                     YRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIM
                     ECDKLIALPPAANQSSVARIA"
BASE COUNT         3574 a         2269 c         2785 g         3388 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgcgcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtatacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg
      361 tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga gccttatagg cgcatttgaa
      421 aaaactccag ctataccgga agatatttat tattaacagg agaggtttgc ttttctgtgt
      481 aggtttagat tttgtgtttg cagggtaaac aaaactgatt aactcgtcat ttcggtaact
      541 gaaatagttt gctgtattct gtttacaatc tctttaataa ggcatttgtc acgtggctat
      601 tcatatgaaa atcatacgtt atcttacaaa tcaatcatct gagtgttata atataactta
      661 gctgtgatat gtgtaagaat gtttaggcaa tatattatta tcgctaatgt tgttttggtt
      721 gggagagatt ctccttgcgg tagctttttg tttttagatg cttcttgctt aaaaagatat
      781 aatttctgtt cttttatata ggattcttta catttactga gttttcttat ttatttaaac
      841 attatgaaat ctaatgcttt tgacgtcatg gggaaggttg catggctatg ggcctgttct
      901 cctctgcata aaaaatggcc attatctgtt tttgctatta atgtcatacc tgctattcag
      961 acaaatcaat ttgctctctt aataaaggat gagcttcctg tagctttttg tagctgggcc
     1021 agtttagatc tggaatgtga ggtaaaatat ataaatgatg taacatcttt gtatgcaaaa
     1081 gactggatgt cgggagaaag aaagtggttt attgactgga tagctccatt tggacacaac
     1141 atggagttat ataaatacat gaggaaaaag tatccttatg agttattcag agcaataagg
     1201 ttagatgaga gcagtaaaac aggaaaaata gctgaatttc atggaggggg gatagataaa
     1261 aaactggcat caaaaatatt caggcaatac catcatgaac ttatgtctga agtaaaaaac
     1321 agacaagatt ttaattttaa tattgagaaa gaaaactaat atgacagtaa ataaaataaa
     1381 gaacattttc aataatgcga cattgactac aaaatcagca tttaatacag catcatcaag
     1441 cgtacgttcc gctggcaaaa aactcatatt attaatacct gataattatg aagctcaggg
     1501 cgtgggtatt aatgagttgg tcaaagctgc tgatgagctt ggaatagaaa tacaccgtac
     1561 tgaacgagat gatacagcga ttgcaaacca gttttttggt gcagcagaaa aagttgtagg
     1621 attaactgaa cgtggtgttg caatattcgc accacaactt gacaaacttc tgcagaagta
     1681 tcagaaagtt gggagtaaaa taggaagaac cgctgaaaat gtaggtaata atctgggaaa
     1741 agccggaaca gttctctcag cactacagaa ttttacgggg attgctttat caggcatggc
     1801 tcttgatgaa ttgctgagaa aacaacggga aggagaggat ataagtcaga atgatattgc
     1861 caaaagtagt attgaactta ttaatcagct tgtagataca gtatcaagta taaacagtac
     1921 cgttgattca ttttctgagc agcttaacca gcttggctca tttttatcca gtaaacctcg
     1981 cttaagttct gttggtggga aattacaaaa tttaccagac ctgggctccc tgggggatgg
     2041 gctggatgtt gtctccggaa ttctttctgc tgtatcagca agctttattc tgggaaacag
     2101 tgacgcacat acaggaacaa aagctgcagc gggtatcgaa ctgacaactc aggttcttgg
     2161 aaatgttggt aaagctgttt cgcaatatat tctggctcag agaatggcac aggggttatc
     2221 gacaacagct gcaagtgcgg gtctgatcac atcggctgtt atgctggcta tcagtcctct
     2281 ttctttcctg gctgctgcag ataaatttga gcgagctaag cagcttgaat catattctga
     2341 acgatttaaa aaattgaatt atgaagggga tgctttactc gcagcctttc ataaagaaac
     2401 cggagctata gatgcagccc tgacaacaat aaatactgtc ctgagttctg tatctgcggg
     2461 agttagtgca gcctccagtg catccctcat aggggccccg ataagcatgc tggtgagtgc
     2521 attaaccggt acgatatctg gcattctgga agcatcaaaa caggctatgt ttgagcacgt
     2581 tgcagagaaa ttcgctgctc ggatcaatga atgggaaaag gagcatggca aaaattattt
     2641 tgagaatggc tatgacgcaa gacatgctgc gtttttagaa gactctctgt ctttgcttgc
     2701 tgatttttct cgtcagcatg cagtagaaag agcagtcgca ataacccagc aacattggga
     2761 tgagaagatc ggtgaacttg caggcataac ccgtaatgct gatcgcagtc agagtggtaa
     2821 ggcatatatt aattatctgg aaaatggagg gcttttagag gctcaaccga aagagtttac
     2881 acaacaagtg tttgatcctc aaaaagggac catagacctt tcaacaggta atgtatcaag
     2941 tgttttgaca tttataacac caacatttac cccaggagaa gaagttagag aaagaaaaca
     3001 gagtggtaaa tatgaatata tgacatctct tattgtaaat ggtaaggata catggtctgt
     3061 aaaaggcata aaaaatcata aaggtgtata tgattattca aaattgattc agtttgttga
     3121 aaagaataac aaacactatc aggcgagaat gatttctgaa ctcggagata aagacgatgt
     3181 ggtttattct ggagcaggct catcagaagt atttgctggt gaaggtcatg ataccgtatc
     3241 ttataataag acggatgttg gtaaactaac aattgatgca acaggagcat caaaacctgg
     3301 tgagtatata gtttcaaaaa atatgtatgg tgacgtgaag gtattgcagg aagtcgttaa
     3361 ggaacaggag gtgtcagtag ggaagcgaac agagaaaata caatatcgtg attttgaatt
     3421 cagaaccggt ggaattcctt atgatgtaat agataatctt cattctgttg aagagctcat
     3481 tggcggaaaa catgatgatg aattcaaagg cggtaagttt aatgatatat tccatggcgc
     3541 agatgggaac gattatatcg aaggtaatta tggtaatgat cgactatacg gcgatgatgg
     3601 ggatgattat atatccggag gacagggaga cgaccagtta tttggtggta gtggaaacga
     3661 taaattgagt ggaggggatg gtaataatta tctgacagga ggaagcggta atgatgagct
     3721 tcaggcacac ggagcttata atattctgtc aggtggtact ggtgatgata aactttatgg
     3781 tggtggtggt attgatcttc tggatggagg ggaaggtaat gactatctga atggtggttt
     3841 tggtaatgat atttatgttt ataggcaaaa ctatggtcat catacaattg cagatgaagg
     3901 aggtaaagga gatcgtttgc acttatctga tattagcttt gatgatatcg catttaagag
     3961 agttggaaat gatcttatca tgaataaagc cattaatggt gtactttcat ttaatgagtc
     4021 aaatgatgtc aatgggataa catttaaaaa ctggtttgcg aaagatgcct caggagcaga
     4081 taatcatctt gttgaggtta taacagataa agatggtcga gagataaaag ttgataaaat
     4141 acctcataat aataatgaac ggtcaggtta tataaaagcc agtaatatag catctgaaaa
     4201 aaacatggtt aatatcacca gtgttgccaa tgatattaat aagattattt cttcagtttc
     4261 agggttcgat tcaggtgatg aacgattagc atctttatat aatttatcct tacatcaaaa
     4321 caacacacac tcaacaactt taacgacaac tgtctgagac taaaagagga ggtgatggcc
     4381 attccaggag gaatggtcat cgttatatga tgagtaaatg tagttctcat aatagtctgt
     4441 atgcactgat attgcttgca caatatcata atataactgt caatgctgaa actataaggc
     4501 atcagtataa tacccacaca caagattttg gggtgactga atggttactg gcagcgaaat
     4561 ccattggctt aaaagcaaaa tatgtagaaa aacatttttc cagattgtca ataatttctt
     4621 tacctgcgtt gatatggcgg gatgacggta agcattatat attgtctcgt gttactaaag
     4681 attcatcacg ctatcttgtt tatgatccag aacaacatca gtcactaact tttagtcggg
     4741 atgagtttga aaaactgtat cagggaaaag tcattctggt tacgtcaaga gcaacagtag
     4801 tcggagagtt agctaaattt gatttttctt ggtttatccc ctctgttgtg aaatacagga
     4861 ggattttact tgaggtgtta actgtttctg cttttattca gtttcttgcg ttaataacac
     4921 ctcttttttt tcaggttgta atggataagg ttttagttca ccgggggttt tcaacgttaa
     4981 atattatcac aatagcattt attatagtga tactttttga agtgatatta accggagcca
     5041 gaacttatat tttctctcat actacaagtc gtattgacgt cgaactgagc gctaagttat
     5101 tcagacattt gcttgcattg cctgtttcat attttgaaaa tcgcagggtc ggagagaccg
     5161 ttgccagagt aagggaactg gagcaaattc gtaatttttt aaccggacaa gcgttgacat
     5221 cagttcttga tctatttttt tctgtaatat ttttttgtgt catgtggtat tacagccctc
     5281 aattaacact ggttatatta ttgtcactac cttgttatgt tatatggtca ttgtttatat
     5341 cacccttatt acgtcgacgt cttgatgata agtttctcag gaatgcagaa aatcaagctt
     5401 ttcttgtcga aacggtaaca gcaataaata caattaaatc catggcagta tcaccacaga
     5461 tgattgctac atgggataaa caactggccg ggtatgttgc ttccagtttc agggtaaatc
     5521 tggttgcaat gacagggcag caggggatac agctgataca gaaaagtgta atggtaatta
     5581 gtctatggat gggagcacat cttgtcatat cgggagagat aagtatcggg cagttaatcg
     5641 cgtttaatat gcttgcaggt caggtcattg ctcctgttat cagactggct catctttggc
     5701 aggattttca gcaggttggt atttctgttg agcgccttgg tgatgtatta aatacaccgg
     5761 tagagaaaaa gtcaggcaga aatatactgc cggaaattca gggggatatc gaatttaaaa
     5821 atgtcagatt tcggtattct tctgacggta atgttatttt gaataatatt aatttataca
     5881 tatcaaaggg ggatgttatc ggtatagttg gccgttctgg ttcaggaaaa agtacattaa
     5941 caaaactgct tcagcgcttt tatataccag agaccggaca gattttaatt gatgggcatg
     6001 atttatcact tgcagatcca gaatggttac gacgccagat tggtgttgta ttgcaggaaa
     6061 atatactatt aaatcgtagt attatcgata atattacatt agcttctccg gctgtatcta
     6121 tggaacaggc tattgaggca gccagacttg caggtgccca tgattttatt acagaactaa
     6181 aagaagggta caatactatt gttggagaac agggcgttgg tctttcagga gggcagcgcc
     6241 aacggatcgc aatagcccgg gctcttgtta caaatcctcg aattcttatt tttgatgaag
     6301 caaccagtgc tcttgattat gagtcggaaa atataataat gaaaaatatg tcaagaatat
     6361 gtaagaacag aaccgtaatt attattgcac acaggttgtc aactgtaaaa aatgcaaaca
     6421 gaataattgt tatggataat ggctttattt ctgaagatgg cacacataaa gagcttatct
     6481 ccaaaaaaga cagtttatat gcatatttat atcagttgca ggcataacgt atgcgctttt
     6541 acatgaaagg tttatgggat ttggtttgcc gttataagac ggttttttct gatgtctgga
     6601 aaattcgtca cacacttgat gctcctgtga gagaaaaaga tgagtatgca tttcttcctg
     6661 ctcaccttga actcattgaa acaccagtat ccagacgttc tcattttgtt gtatggagta
     6721 ttttattatt tgtaattata tctcttctct tatctgttct tgggaaagtt gaagtggttt
     6781 cagtagcaaa tggtaagttt actcatagtg gaagaagcaa agaaataaaa ccgatagaga
     6841 atgcgattgt agaaaaaata atggtaaaag atggctcttt tgtaaaaaaa aatgatccat
     6901 tagttgaatt gacggtgcct ggtgttgaat ctgatatttt aaaatcagaa gcatctttgt
     6961 tgtatgaaaa aacagaacaa tatcgctacg caattctttc tgaatcaata caacggaatg
     7021 agcttcctga aataaggata acagattttc ctggcggaga agataacgcg ggaggtgaac
     7081 attttcagag ggttagctca ttaataaaag agcaattcat gacatggcag aacagaaaaa
     7141 atcaaaagca gctcacatta aataaaaaaa tagttgaacg ggatgcagcg cttgctcgtg
     7201 ttagcctgta tgagcatcag gtatcacagg aaggaagaaa actcaatgat tttaagtatt
     7261 tgttgaataa aaaagctgtt tctcaacatt cagttatgga gcaggaaaat agctatattc
     7321 aggcaaaaaa tgaacatgca gtctggcttg cacaggtttc tcaacttgaa aaagaaatag
     7381 aacttgtgcg ggaagaactg gcactggaga cgaatatctt cagaagtgaa attatcgaga
     7441 agcacagaaa atcaacagat aacattgtgt tgctggagca tgagcttgaa aaaaacaggc
     7501 agagaaaagc atcatctttt attaaagctc ctgtgagtgg tactgttcag gagttaaata
     7561 tacatacaga aggtggtgtt gtcacaacag cagaaacgct gatgattatt gtccctgata
     7621 atgatattct cgaagtaaca gcatctgtac ttaacaagga tatcggtttt atacaacctg
     7681 gacaggaggt cgttattaaa gtagatgcat atccatatac acgtcatggt taccttacag
     7741 ggaaagtaaa aaatataact gcagattctg tttctgttcc ggatacaggg cttgtattta
     7801 acgtgattat atctgttgat cggaatgata tacagggaga aagaaaaaaa atccctgtta
     7861 cagctggaat gactgttatg gcagaaataa aaaccggggt gcgtagtgtt atcagctatc
     7921 ttcttagtcc attaaaggaa actattaatg aaagtttacg tgaacgttag tatataaata
     7981 acatattggc tttttatatg atatagattt atgaaataat ctcgtaaaga ttatctatgt
     8041 tatttaaaca agctatgata cagtttttat ctttaaaaag agttaatgga atggatagta
     8101 ttaaattact tgataaagtt attatctatc aacaacagaa tgagcaatta tatccagcac
     8161 aggaacatct tcttttacaa tgttcatatg atgataaata cgattttaac gccagcactc
     8221 accgtggcat catcggagag tcgctcacaa ggctcggggc gatgttttct ggtaaagagt
     8281 accagatact gcttcctggt gaaattcaca ttaaagaaag tggtaagcga gaatataagg
     8341 gccgggattc cggccttttt atgcagcctt tataacagtg tattagtcat tcgcatattc
     8401 aacatatccc tcctcacaat ggtatatgcc ataatacatt ctcttaccct ccgggccttc
     8461 agctttaaat gggacaagaa gtgttttttc atcttcaaca tctgttaaac ttctctcaat
     8521 cttttcccat gtaattttcg ggatttctgt accaagaagt tgaacatcgt tgtcccagcg
     8581 tttcattctt tgcaggtaat cattttttac aaacgttctg gcctgttcat cgctgacctg
     8641 atagcaggcc ggacctttat cttcagacca gaaaaaaaca gcagcaagag caataatcgt
     8701 taaaataaaa ataaatttca tgctaattac tttcataaaa tctccacaac ttctgtcgtt
     8761 cattcgatat aaaaaaacat tacgaatata tccgtacaat gtgagcatac tgtgaagtgg
     8821 ctttaacaat tatgaaataa tacaagagat cacatattgc agtggtctct tttttataga
     8881 agaaatcaca attcataatt gtgacttatt atgcatgttt gtttttcagc attgtgaccg
     8941 catcggactc aggcatcgaa aactgcaccc ggtgccgggc agcgacatcc agcgcaaaca
     9001 ccttcgtgta gacctccgtt gagctgatgg acttatgccc catcagactc tgcaggacct
     9061 tcagcggtat accggcatac agcatatgca tcgcatagga atggcggaac gtatgtggcg
     9121 tgaccgggac agagaacgtc acaccatcag cggcagcggc ggcaaccgcc tccccaatcc
     9181 aggtcctgac cgttctgtcc gtcacttccc agatccgcgc tttctctgtc ctgcctgttc
     9241 gtttattacg ccgttccatg gggattttca atgtggccac catcgtctgc agctggctga
     9301 cgtaccagga gtcggagagc ggaaccagcc ggtgagtctg ctgcccggcg ggcgttcttc
     9361 ctgccgtcct ggcggctttt tcggtccgtt gtttcagggt cgcaagctgc acaaacggat
     9421 acggaggcgt gagcgaaaaa tccccccgcg tcagcgccag tgcttcatta atgcgcgcac
     9481 cggtgttcca cagtgtggcc agcagcatct tgcggcgcag atccgggacg taatggagca
     9541 gggcgctcac ttcaggggcc agcaggtatt tgggcagttc atcatgaacc atcgacatct
     9601 ggcggagtgc cagcgctgcc ggataatcga tggcaacagg aagctgagcg gcatcccggg
     9661 catggtggat cggtgccggc aggtgctgca tatcagtgtt cactccacgg ggaaacgtcg
     9721 ggcaggtttt ccacaacccg gtactttccc ggataacgga gctggttacc gccacgcgtc
     9781 cagacggtga caacaaaacc ccctccctgg ttgttttcgg ggagcaggcg atcgcactgc
     9841 tcactcagca tgtggatcac gaacggcgca tcgctgccgt cttcaaatat cagttccagc
     9901 gcatcgcggc catgcagcgt tcccttactg atgatgacgt actcagcccc ccgcatctcc
     9961 ctgagtaaat gttttgccgc atccgggaca agcagcctgg ctgcgccggc attccagctg
    10021 aggtagacat atccggcctg cgcctgtaca gactgccagt aatccgttct ggcgacagcc
    10081 tgaccttcat tctcaataaa aaacatgggt gactcctttt ttcttttccg ggcagggacg
    10141 gtcatgcgat aacgtcatcc atcatcactg ctcttaccgt cacgccatgt gataacatca
    10201 tgccgttatc atcatcacca tgataacaag catccatata accacgctgt taaattaccg
    10261 gaggaccatt tccgacagat ccgatttttc tgttattgac gggttttcgg aagtgtatca
    10321 cgctgaggaa ataatcatat cgctgttttc cgcccctgcg cgacgcatca caggtcaggg
    10381 agatgacaca acatatagtg tcatcagcag atgaatccgg caccacatgt tgtgatgacg
    10441 ggaggtggcg ttgtgtgggg ggagaggaag atcctgaaca accttttttc ggatctccga
    10501 taaaaaagaa agaaatagag atatgctaaa catatgcctg gcatctacat atgctaccgc
    10561 tatacatatg ccacacatct gcctatacta aacatatgca cacacccggt gctttgaata
    10621 ttccggatat gtttcacata tccatatgct cgggagagaa agaacatgag aaccgtatcg
    10681 atatttaaaa atggcaacaa ccgcgccatc cgtctgcctc gtgatctgga ttttgagggg
    10741 gtgagcgagc tcgagatcgt ccgggaaggg gacagcatca tcctgcgccc cgtccggcca
    10801 acctggggat cattcctaga gtacgaaaaa gccgatccag attttatggc ggagcgcgag
    10861 gatgttgtca gcgacgaagg gcgatttaac ctgtgaacaa gacttatatg cttgatacct
    10921 gtatctgctc gttcatcatg cgtgaacagc cagaagccgt cctgaagcgt ctggagcagg
    10981 cggtgctacg cggccaccgc atagtggtct cggccatcac ctactctgaa atgcgcttcg
    11041 gggctaccgg cccaaaggcc tcaccacgtc acgtccaact ggtcgataaa ttctgcgccc
    11101 gcctcgatgc catcctgccc tgggatcgcg ccgcagtaga cgccaccacg aagattaaag
    11161 tggcactgcg gctggccggg acgccgatcg gcccgaacga cacggcgatt gccgggcacg
    11221 ccatcgccgc cggggcgata ctggtaacga ataatacaag agaatttgag cgggtgcctg
    11281 acctggtgct ggaagactgg gtgaagtaag acactctttt caaagtcagg agataagggc
    11341 accaagaaac agcccgtata cgggctgttt taacagatat ctacagtaag cggtcctaaa
    11401 ctgtagggta tcttcgaaga tcagactatc agggagctaa agcctatctt ccggcggcaa
    11461 aaacagtggc tcaagcgata cgggcaaccg agctctggtt tgctgccggt ggcagggcga
    11521 tgagcttgtc acattccata atcacgtact ccgggatgag atcgaatctg gacggatcta
    11581 gttgacagat gaactcattt tcaatgtggt acgtttcgtt aacgaatttt cgtattaccg
    11641 gatggtcctg atccagttcc agtaaacgga atacctgcag tttttcatag ccgttacgac
    11701 agcgacggta caggtttttt atttcatctg ggctgctgac gagagcctga agtcgcgggt
    11761 aatcaaaact gtccacatac tcccggatat cccgcaaggc ctgctgaatt ttctcaggag
    11821 ccattttggg gtagcccgag cccgcagcgg caggctcgcg ataatccagc ggggcgacgc
    11881 gtcgatggaa tagattggat agaagctgat aagcatcacc acgttcgtca acgatttcaa
    11941 agtagcgacg aagatagatg agcttaatga tctcctcaca gtctgctgac gcagtgatgg
    12001 atttgcagat ctgcat
//
LOCUS       DAZKGS010000132        11874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKGS010000132 DAZKGS010000000
VERSION     DAZKGS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(227..874)
                     /gene="ychE"
                     /locus_tag="ACHOQY_003653"
     CDS             complement(227..874)
                     /gene="ychE"
                     /locus_tag="ACHOQY_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9679679.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(905..958)
                     /gene="ychE"
                     /locus_tag="ACHOQY_003654"
     CDS             complement(905..958)
                     /gene="ychE"
                     /locus_tag="ACHOQY_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9679680.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1351..4026
                     /gene="adhE"
                     /locus_tag="ACHOQY_003655"
                     /gene_synonym="adhC"
     CDS             1351..4026
                     /gene="adhE"
                     /locus_tag="ACHOQY_003655"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9679681.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4150..5046
                     /locus_tag="ACHOQY_003656"
                     /pseudo
     CDS             4150..5046
                     /locus_tag="ACHOQY_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5328..5945)
                     /gene="tdk"
                     /locus_tag="ACHOQY_003657"
     CDS             complement(5328..5945)
                     /gene="tdk"
                     /locus_tag="ACHOQY_003657"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9679682.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6550..6963
                     /gene="hns"
                     /locus_tag="ACHOQY_003658"
     CDS             6550..6963
                     /gene="hns"
                     /locus_tag="ACHOQY_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9679683.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7107..8015)
                     /gene="galU"
                     /locus_tag="ACHOQY_003659"
     CDS             complement(7107..8015)
                     /gene="galU"
                     /locus_tag="ACHOQY_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9679684.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8217..9230)
                     /gene="rssB"
                     /locus_tag="ACHOQY_003660"
     CDS             complement(8217..9230)
                     /gene="rssB"
                     /locus_tag="ACHOQY_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9679685.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9322..10227)
                     /gene="rssA"
                     /locus_tag="ACHOQY_003661"
     CDS             complement(9322..10227)
                     /gene="rssA"
                     /locus_tag="ACHOQY_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9679686.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10340..10798
                     /gene="ychJ"
                     /locus_tag="ACHOQY_003662"
     CDS             10340..10798
                     /gene="ychJ"
                     /locus_tag="ACHOQY_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9679687.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10848..11690
                     /gene="purU"
                     /locus_tag="ACHOQY_003663"
     CDS             10848..11690
                     /gene="purU"
                     /locus_tag="ACHOQY_003663"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9679688.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3214 a         2942 c         2696 g         3022 t
ORIGIN      
        1 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
       61 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
      121 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
      181 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
      241 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
      301 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
      361 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
      421 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
      481 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
      541 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
      601 tcgctatcgt caccaccagg atgcccccgg cgatacggaa cgaatcaatt gatataccaa
      661 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
      721 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
      781 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
      841 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
      901 aatgttagcc ataaataagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
      961 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
     1021 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
     1081 agcgatgctg aaaggtgtca gctttgcaaa aatttgattt ggatcacgta atcggtaccc
     1141 agaagtgagt aatcttgctt acgccacctg gaagtgacgc attagagata ataactctaa
     1201 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
     1261 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
     1321 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
     1381 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
     1441 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
     1501 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
     1561 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
     1621 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
     1681 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
     1741 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
     1801 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
     1861 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
     1921 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
     1981 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
     2041 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
     2101 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
     2161 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
     2221 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
     2281 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
     2341 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
     2401 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
     2461 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
     2521 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
     2581 ttcaaactcg caccttccct gactctgggt tgtggttctt ggggtggtaa ctccatctct
     2641 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
     2701 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
     2761 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
     2821 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
     2881 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
     2941 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
     3001 gccgcgaaga tcatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
     3061 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
     3121 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
     3181 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
     3241 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
     3301 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
     3361 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
     3421 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc gactatcgcg
     3481 ggtatcgcgt ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
     3541 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
     3601 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
     3661 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
     3721 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaggc tgaactgggt
     3781 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
     3841 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
     3901 atctccgagc tgaaacagat tctgctggat acctactacg gtcgtgatta tgtagaaggt
     3961 gaaactgcag cgaagaaaga agctgctccg gctaaagctg agaaaaaagc gaaaaaatcc
     4021 gcttaatcag tagcgctgtc tggcaacata aacggcccct tctgggcaat gccgatcagt
     4081 taaggattag ttgaccgatc cttaaactga ggcactataa cggcttccac aacagggagc
     4141 cgttttctta tgccacttct taatgatctg ctcgatttca gtgaccatcc gcttatgcct
     4201 ccgccctctg cacaactatt tgcagaacac cttcccaccg agtggataca acactgcctg
     4261 acgctttctg ctcatgcgac cgttcgccgc cgtcgtttac cgggggacat ggttatctgg
     4321 atggtggtgc aatgagccaa ttaccgatgt tgttcgccgt ctgaacctga gcgcggatgg
     4381 cgaagcgggg atgaacctgc tggcccgcag cgctgtcacc caggcgcgtc agcgcgtggg
     4441 ggccgcccca gtggaatggc tcttccgcca gaccgcacag acaggggcgc ggaacgttac
     4501 ctgaaggatg actggcacgg cctgcaactt tttgccattg atggcgcaca gttcaggaca
     4561 cctgataaac ccgagctgcg tgaatattat gggtctgcca acacatccac taagcggcag
     4621 aacgcctacc cggttatgcg tctggtggcc ttaatgaacc tgggaagtca cattctgctg
     4681 aatgccgtga ccgcacctta ccggcagagc gaaactgtgc tggcccactc catgctcgcc
     4741 accatcccgg ataactctat tacgttgttt gataaacttt tttacagcga agacctgctg
     4801 ctgacgctga accagaaggg ttgtaaccgc cactggctac tgcctgcgtg gaagaatatc
     4861 gcatcagaaa tgatagagct gggaaacacg gcgtcaacag ggaccatccc gaaacgactg
     4921 gagcatcttc ggggtgctct ggaagtggtg ttcataacaa aacgcccccg accatcaagg
     4981 ccgaggtcgg ttaagatatc aaaaacccgt tatcctgtga aacatagtgc cgctcctctt
     5041 aagtgaacgg cattgccctt ctggggccgt attttttgtt tacccaaagc aacttttcca
     5101 taaaccgaca gcattagcct tcatcatatt tgcgacgatg tataacgcct aaacacaggg
     5161 atattgtact ttacaggtca caagtcaacg tcggtgctta agagccctgt gaggcgtata
     5221 gcggcgttaa aaaactgccg agaagggtat atagcccgga agaagtgcgt aaaacgaact
     5281 gacaggataa aagtgccctg ctcaccctgt cagtaaagaa attcttatta atcgtggcga
     5341 tgcctttcct gaatagccgt taatgagccg acttgtaacg cctctttata gtgtttacgg
     5401 catacagaaa cgtatcgttc attaccacca attaccacct gctcaccttc gttataaggt
     5461 ctgcctgctt gatcaagacg cagcaccatg cttgctttac ggccacaaaa acagatggtt
     5521 tttaattcaa ccagtttgtc ggaccatgcc agtaagtatt ggctgccaat aaataattca
     5581 cctcgaaaat cggtacgtaa accataacaa agtacgggta tatcgagttg atcgacaacc
     5641 tccgataatt catatacttg ttgtctggtt aaaaactggc attcatcaac cagtacgcaa
     5701 tgaattgcct gctgttcatg ttccgcacga atctcatcaa ataatgatga attttggtta
     5761 aataattttg caggcgatga caaacctata cgcgaactga ctttcccggc accaaagcga
     5821 tcatcaattt ctgccgtata tacgacagtg cgcatgccgc gttcctggta attgtatgaa
     5881 gattgcaaca atgctgtaga cttacccgca ttcattgcgg aatagtagaa atatagctgt
     5941 gccatcagcc acaggccctc aatgatatga aataaagttg gctggagttt atcataattc
     6001 gtcagttttt cagctcaaca gtatgcacag aagtgggtat atgcgttctc ccttacgaag
     6061 ccttgcataa tccttctgag ctatcattac aactgcctcg cttgttataa gcgggtaaat
     6121 gactgctggt aactattcac aatctttaac ctgttgcgca agtaatagcc ctctgttgac
     6181 ctccaggaga tagtgcaata ctaagtccat gctcttattg cgactgttct acttttcatc
     6241 attcgcttaa tagggaattc tcgtaaacac aactaataca gaagactgaa aggtcgtcag
     6301 cctacgataa tctccccata aaatgtgaca tgaatcagga agttttaacc tcacgtgctg
     6361 cgaaatcatc ggtgtaaata gggctatatg ccgcgtcttt tctggctaat tttatgaaaa
     6421 gatatttatt ggcggcacaa aataaagaac aattttgaat tccttacatt cctggctatt
     6481 gcacaactga atttaaggct ctattattac ctcaacaaac caccccaata taagtttgag
     6541 attactacaa tgagcgaagc acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag
     6601 gcaagagaat gtacacttga aacgctggaa gaaatgctgg aaaaattaga agttgttgtt
     6661 aacgaacgtc gcgaagaaga aagcgcggct gctgctgaag ttgaagagcg cactcgtaaa
     6721 ctgcagcaat atcgcgaaat gctgatcgct gacggtattg acccgaacga actgctgaat
     6781 agccttgctt ccgttaaatc tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat
     6841 agctacgttg acgaaaacgg cgaaactaaa acctggactg gccaaggccg tactccagct
     6901 gtaatcaaaa aagcaatgga tgagcaaggt aaatccctcg acgatttcct gatcaagcaa
     6961 taatcttttg tagattgcac ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc
     7021 ggtttaagac aatttaataa gaataagcta taaaaaaacg gcatcgattg ctcaacgccg
     7081 tttcgtggat aacaccgata cggatgttac ttcttaatgc ccatctcttc ttcaagccag
     7141 gctttaaatt ccgtgccaag ggtgttatga cgaataccgt attcaacgaa ggcctgcatg
     7201 taacctaatt tattaccgca gtcatggctc ttccctttca tatgataggc ttccaccgtt
     7261 tctttttcaa tcagcatatc aattgcgtcg gtgagctgaa tttcatcacc agctcccgga
     7321 ggggtttttg ccagcaacgg ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg
     7381 agattagacg gcgcaacatc cgctttcggt ttttctacca caccaaccat cggtacgctt
     7441 tcacccggcg ctaattcaac gcctttgcaa tccacaacgc catatgcggt cacatcagca
     7501 accggttcaa ccatgatctg gctatgaccc gtttcatcaa agcggcggat catctctgcc
     7561 aggttatcct gtgacaaatc ggattcatat tcatccagaa taacatcagg caaaataaca
     7621 gctaccggtt catcacccac taccgggtga gcacacaata ccgcgtgtcc caggcctttc
     7681 gccagaccct gacgaacttg cataatagtc acgtgcggtg gacaaataga ctgcacttca
     7741 tcaagcagtt gacgttttac acgtttttcc agcattgctt ccagttcaaa actggtatca
     7801 aagtggtttt caatagagtt tttagatgag tgtgtaacca gcacaatttc agtaatgcca
     7861 gccgcaatac attcattcac gacgtattga attaatggct tatcgacaag tggcagcatc
     7921 tctttcggga tggctttcgt cgccggcaac atcctggttc ctaatcccgc aacggggata
     7981 acggcttttt tgactttcgt attaatggca gccatttaaa ttctcctgga ctgttcgtgt
     8041 tttgaacgtg ttcatatttc tgtatcgcat cccagtatat cagcaccccg aaggggctaa
     8101 gttctgaaaa ggaggcgagt tagcataatt aagacaatta cttatacgaa tggcgacgat
     8161 agtaccacca gccgacatta gcaggtaatg caaatttagc ccgcgttatc gtttgctcat
     8221 tctgcagaca acatcaagcg cagtcgacca ccggttcccc atatttggca ttgccaggca
     8281 tcgcatcgct ggctcaattg attcaaataa gcgttaccta aagtgcctaa cggaacacca
     8341 ttactgattt gcacctggtg ttcgccggta tttaacgtcg cattcagacc cgcagaaacc
     8401 agaatgagat ttttcagttc gcgatgataa tagccaacta atagcggaaa ctgccccggc
     8461 agattggcct gacgaagtaa atggtttacc tgcttcaata acgcgcccaa ctctggcaac
     8521 cgttgatttt ggtgtgcaag ctgttcctgt aataatccgt taaacaatgc gcgtaataac
     8581 aaggcagcaa gtacgccatt atgtccagct cgggtgacat caaggcaata aaatgccaga
     8641 tcgttttccg aaagtgcggc aatatcaagc accaggccgg gtttgtccgc ggcaaccaat
     8701 tgacgataat taacccggca atgggaaatc acctgctgaa ccggcggttg tagttcctgt
     8761 aataatttcg ccgctgcggc agggttatca accattgcat cccagtcgcg aaaaagcctt
     8821 tcctcttcct caacgcgcga attaaacatg ctgggataga gacaggcaaa aaccatctcg
     8881 cgcaagcgat tcaaatcttt aactggtttc agcaaaacat cttcaacgcc cagacgtaac
     8941 gctttggcaa tatctgccat attttcggtg gcagatatca ccagaaccgg tgtctggtca
     9001 cctctgttgc gtatatgttc cagcagttta agcccgttca ttcgtggcat cgcgatatca
     9061 catatcatca ggtctggagt gaaacctccc agcaactcaa gggcatccac cccatcagcc
     9121 gccagtaccg ttgtcgctcc caatgaggaa aaccacgaat ccagaagcga gcgaaatacc
     9181 ggctcatctt caacaatgag aatctgtttt ccgaccaatg gctgcgtcat gttctctccc
     9241 ctgactggct ttactcaata gtggcatgct attgccacct gcgcctgtca gaattaactt
     9301 aagtgtagat aaaaattctg gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat
     9361 tttcttttcc actgccagct gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag
     9421 cgtagatatt tgcgggcaaa caggttgaat cagaatatcg ggcggatcac ctgccatgcg
     9481 gttcctttta aggcggttct ccagcacctg gatagaagtg gtcataatct ctgttgccgt
     9541 tggcgctgtc accgcccgac gtgtcgttat gctgcctaac ctttctttca gacgcgcatg
     9601 ccacggcaga gaatcaccat tctcgctatt ttcttcactg acattaaagg agagcaaatc
     9661 ttgttgcatc aaatgagcat cgtgctgcag gtcaaccgct atcacaatat cagcccccaa
     9721 tgcacgcgtg agggaaatag gaattgggtt aacgactgct ccatcaacca gccagtagcc
     9781 gttatgtgca acaggtgcca tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg
     9841 gagatcgcct tcagtaaacc ataattcacg tcccgtactt aaattggtgg caacagccgc
     9901 aaagcgacgg gaacaatttt cgatctctgt ttccggcatt atttcgcggt attgattgaa
     9961 gacacgctcg ccgcgcagta acccaccgcg ctgccaggag agatccatca ggcgtaaaac
    10021 atcccaataa ctgaaagagg tcacccaatc ttccagcgca gataatcgat cgcatgcata
    10081 ggcagcgccc accagcgaac caattgaaca tcctgcaacg atatcaattt caatacccac
    10141 tttttttagc gcattaataa cgccaatatg cgaccaacct ctcgccgcgc cagatcccag
    10201 cgccagccct atttttatct ttctcattat tcccgcaaga ttcccctgga atgcaatcgt
    10261 agccacatcg ctactgctcg gttaacatag tgctactcag gcggttaata cgccgttatt
    10321 gtttccaggg agattatttg tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag
    10381 cctatgttgc cacccttatg tgtctggtga aaaggttgca cctgatccag aacatctcat
    10441 gcgttcgcgt tactgcgctt ttgtgatgca agacgcagat tatttaataa agacctggca
    10501 tccctcttgt ggagcagcgg cgttacgtgc cgaattgatg gccggatttg cgcatactga
    10561 atggttggga ttgaccgtat ttgaacattg ttggcaggac gccgacaata tcggatttgt
    10621 cagttttgtt gctcgattta ccgaaggtgg taaaacaggt gcaataattg aacgttcgcg
    10681 atttttgaaa gaaaacggtc aatggtacta tattgacggt acacgtccgc agtttggtcg
    10741 caacgatccc tgcccttgtg gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg
    10801 gttgacggta cggtttagca aacactctca acaaggtttt tccagcaatg cattcactcc
    10861 aacgtaaagt tctgcgtact atttgtccgg accaaaaagg tctgatcgca cgtattacca
    10921 atatttgcta caagcacgag ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta
    10981 ccgggcgctt ttttatgcgc acggaactgg aagggatttt taatgattcc accctgctgg
    11041 cggatctcga tagcgcattg ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc
    11101 gccggatagt gattctggtc actaaagaag cgcattgcct tggcgatttg ttgatgaaag
    11161 ccaattacgg cggcctggat gtcgaaatcg cggcagttat tggtaaccac gatactttac
    11221 gttctctggt tgagcgtttt gatattccgt ttgagctggt aagccatgaa gggttaagcc
    11281 gcaacgagca cgatcaaaag atggcggacg ccattgatgc ttatcaatct gactatgtgg
    11341 tgctggcgaa gtatatgcgg gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa
    11401 tcatcaatat tcaccattca ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg
    11461 cctatgaacg tggtgtgaag attattggcg caaccgctca ctatgtgaat gacaatctgg
    11521 acgaaggccc aatcatcatg caggacgtta ttcatgtcga tcatacctac acagctgaag
    11581 atatgatgcg cgcaggtcgt gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag
    11641 tactggcaca gcgcgtcttt gtttacggta atcggacgat tattctttaa tcgccagcaa
    11701 aaataactgg ttacctttaa tccgttacgg atgaaaatta cgcaaccagt tcatttttct
    11761 caacgtaaca ctttacagcg gcgcgtcatt tgatatgatg cgccccgctt cccgataagg
    11821 gagcaggcca gtaaaaagca ttaccccgtg gtggggttcc cgagcggcca aagg
//
LOCUS       DAZKGS010000133        11679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKGS010000133 DAZKGS010000000
VERSION     DAZKGS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..2487
                     /gene="ppsA"
                     /locus_tag="ACHOQY_003664"
     CDS             109..2487
                     /gene="ppsA"
                     /locus_tag="ACHOQY_003664"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9679689.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2544..4190)
                     /gene="fadK"
                     /locus_tag="ACHOQY_003665"
     CDS             complement(2544..4190)
                     /gene="fadK"
                     /locus_tag="ACHOQY_003665"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP9679690.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(4247..4540)
                     /gene="ydiT"
                     /locus_tag="ACHOQY_003666"
     CDS             complement(4247..4540)
                     /gene="ydiT"
                     /locus_tag="ACHOQY_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9679691.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4537..5826)
                     /gene="ydiS"
                     /locus_tag="ACHOQY_003667"
     CDS             complement(4537..5826)
                     /gene="ydiS"
                     /locus_tag="ACHOQY_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9679692.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5882..6820)
                     /gene="ydiR"
                     /locus_tag="ACHOQY_003668"
     CDS             complement(5882..6820)
                     /gene="ydiR"
                     /locus_tag="ACHOQY_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9679693.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     AAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6840..7604)
                     /gene="ydiQ"
                     /locus_tag="ACHOQY_003669"
     CDS             complement(6840..7604)
                     /gene="ydiQ"
                     /locus_tag="ACHOQY_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9679694.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7920..8831
                     /gene="ydiP"
                     /locus_tag="ACHOQY_003670"
     CDS             7920..8831
                     /gene="ydiP"
                     /locus_tag="ACHOQY_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9679695.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(8874..10025)
                     /gene="ydiO"
                     /locus_tag="ACHOQY_003671"
     CDS             complement(8874..10025)
                     /gene="ydiO"
                     /locus_tag="ACHOQY_003671"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9679696.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITSAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(10039..11633)
                     /gene="ydiF"
                     /locus_tag="ACHOQY_003672"
                     /pseudo
     CDS             complement(10039..11633)
                     /gene="ydiF"
                     /locus_tag="ACHOQY_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
BASE COUNT         2821 a         2998 c         2919 g         2941 t
ORIGIN      
        1 atatttgctt gaacgattca ccgttttttt catccggtta aatatgcaaa gataaatgcg
       61 cagaaatgtg tttctcaaac cgttcattta tcacaaaagg attgttcgat gtccaacaat
      121 ggctcgtcac cgctggtgct ttggtataac caactcggca tgaatgatgt agacagggtt
      181 gggggcaaaa atgcctccct gggtgaaatg attactaacc tttccggaat gggtgtttcc
      241 gttccgaatg gtttcgccac aaccgccgac gcgtttaacc agtttctgga ccaaagcggc
      301 gtaaaccagc gcatttatga actgctggat aaaacggata ttgacgatgt tacccagctt
      361 gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca ctcccttcca gcctgagctg
      421 gaaaatgcca tccgcgaagc ttatgcacag ctttccgccg atgacgaaaa cgcctctttt
      481 gcggtgcgct cctccgctac tgcagaagat atgccggacg cttcctttgc cggtcagcag
      541 gaaaccttcc tcaacgttca gggttttgac gccgttctcg tggcagtgaa acatgtattt
      601 gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc accagggtta cgatcaccgt
      661 ggcgtggcgc tctccgccgg tgttcaacgg atggtgcgct ctgacctcgc atcttctggc
      721 gtgatgttct ccattgatac cgaatccggc tttgaccagg tggtgtttat cacttccgca
      781 tggggccttg gtgaaatggt cgtgcagggc gcggttaacc cggatgagtt ttacgtccat
      841 aaaccgacac ttgcagcgaa tcgcccggct atcgtgcgtc gcaccatggg gtcgaaaaaa
      901 atccgcatgg tttacgcgcc gacccaggag cacggcaagc aggttaaaat cgaagacgta
      961 ccgcaggaac agcgtgacat cttctcgctg accaacgaag aagtgcagga actggcaaaa
     1021 caggccgtac aaattgagaa acactacggt cgcccgatgg atattgagtg ggcgaaagat
     1081 ggccacaccg gcaaactgtt cattgtgcag gcgcgtccgg aaaccgtgcg ctcacgcggt
     1141 caggtcatgg agcgttatac gctgcattca cagggtaaga ttatcgccga aggccgtgct
     1201 atcggtcatc gcatcggcgc gggtccggtg aaagtcatcc atgacatcag cgaaatgaac
     1261 cgcatcgaac ctggcgacgt gctggtcact gacatgaccg acccggactg ggaaccgatc
     1321 atgaagaaag catctgccat cgtcaccaac cgtggcggtc gtacctgtca cgcggcgatc
     1381 atcgctcgtg aactgggcat tccggcggta gtgggctgtg gtgatgcaac agaacggatg
     1441 aaagacggtg agaacgtcac tgtttcttgt gccgaaggtg ataccggtta cgtctatgcg
     1501 gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa cgatgccgga tctgccgttg
     1561 aaagtgatga tgaacgtcgg taacccggac cgtgctttcg acttcgcctg cctgccgaac
     1621 gaaggcgtgg gccttgcgcg tctggaattt atcatcaacc gtatgattgg cgtccaccca
     1681 cgcgcactgc ttgagtttga cgatcaggaa ccgcagttgc aaaacgaaat ccgcgagatg
     1741 atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc gtctgactga agggatcgcg
     1801 acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc gtctctctga ttttaaatcg
     1861 aacgaatatg ccaacctggt cggtggtgag cgttacgagc cagatgaaga gaacccgatg
     1921 ctcggcttcc gtggcgcggg ccgctatgtt tccgacagct tccgcgactg cttcgcgctg
     1981 gagtgtgaag cagtgaaacg agtgcgcaac gacatggggc tgaccaacgt tgagatcatg
     2041 atcccgttcg tgcgtaccgt agatcaggcg aaagcggtgg ttgaagaact ggcgcgtcag
     2101 gggctgaaac gtggcgagaa cgggctgaaa atcatcatga tgtgtgaaat tccgtccaac
     2161 gccttgctgg cagagcagtt cctcgaatat ttcgacggct tctcaattgg ctcaaacgat
     2221 atgacacagc tggcgctcgg tctggatcgt gactccggcg tggtgtctga attgttcgat
     2281 gagcgcaacg atgcggtgaa agcattgctg tcgatggcta tccgtgccgc gaagaaacag
     2341 ggcaaatatg tcgggatttg cggtcagggt ccgtccgacc atgaagactt tgccgcatgg
     2401 ttgatggaag aggggatcga tagcctgtct ctgaacccgg acaccgtggt gcaaacctgg
     2461 ttaagcctgg ctgaactgaa gaaataaaat aaatccccgg cggcgttcag tcgccgggtt
     2521 tatgtgatcc ccgaagataa aacttattca atctcttcac agacatcctg cgttaatcgc
     2581 cgcataatat cttttcttaa caaaaacttt tgtattttac ctgaggcagt gcgcggtagt
     2641 ttttcgatta ccacgatatg ttcaggatat ttatattttg cgacccgttt acggctaaaa
     2701 aaagccacca cctcttccag cgataatgaa tgatgcggcg ctttcagcac gacataagcg
     2761 catgatcgtt cacctaaacg ttcatcgggc attgcaacca cacaggcatc gtgaatttta
     2821 ggatgctgca ataaaatatc ttccacttca cggctgctaa tattttcgcc gccgcggaca
     2881 ataatatctt ttttgcgtcc ggtaattttt atatagccag cctcatccat acggcagaga
     2941 tcgccgctgt aataccagcc ttcttcatcc agcgcacggg cggttaattc aggttcatca
     3001 aaatatccca taaacacatt ggggccacgc gaggcttctt caccttcgca acctggcggt
     3061 aaggtcttgc gtgcgtcatc gaccacttta atctctacac ctgcggcagc gtaaccatcg
     3121 gtgtgcataa agcgcgacaa aggatcatcg agattcacca ccgcatgcgg cgaactttct
     3181 gtggaaccat aaacacttaa taatttaatg ccgagctgct ggcattcacg cgccactttt
     3241 ttggggattg tggttccgcc gcaaagaaag aaacgcagcg ctgacaggtc cgcgggttgt
     3301 ttctctagta cattcaaaag atcatagaca aacggcgttg cgcctagcat acaggtgcaa
     3361 cgctgctgct caagcagcgc gagacacgca tcaggagtga aaatatctaa caacacgctg
     3421 cgagcgccaa ttaagaatgg tgccgttacg ccatgcagaa agcccgttgc gtggccgagt
     3481 ggcgcaggca tcataaagac atcctgccag gtcagattca gccgcgcgca ataagcccgc
     3541 tcactggcga gaatattgtt atgcgttagc atcacgccct ttggcagacc ctcggttccg
     3601 gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg ttatcgccgt ggtcagtggg
     3661 gtattgtcgg cgataatctg acttaatgag agggaagagg tggcgggagc cagtttgtcc
     3721 acgccgacaa tttgttgtag ttgtggaagc tgattttgca gcggcaggat taaatctacc
     3781 gggcgcgttt gtttaaacaa cgtcggtgca aagaacattt ttgcctgaca cttattgagc
     3841 acccacacca gttctgcttc ccgccaggaa ggcaacagcg gcacggaaac cgcaccgatt
     3901 ttcaggcagg caagatagat aacggtaaat tcacaccagc caggcagttg aaatgcgatg
     3961 cgatcgcctg attcaatacc cttcgctaac atccagtttg ccagacagct cgcggcgtga
     4021 tcgagcgcgc tataggtgta cgatgcacca tgattatcga ccacggcaat tttgtctggc
     4081 atcgcacgag cggtctgctg ccagtaatcg gccagcgaag catcgcccca tagcccttgc
     4141 tgacgatacg ccgcacgacg ttgttcgttg aacgttaatg tcactttcat gttggactct
     4201 cgaaacagaa catcaggccc gagatgcggg cctgtgggat gcatgattag ccgtagcgaa
     4261 aatcaatacc gaaggtgcct gcgggatatt gccactgttc gagaatagtg ttaccgcaca
     4321 gcacccgaca ggtgccgcac tccagacaac cggcagaatc aaaatgaatg ttgccggcgt
     4381 catcctgctt ataaagtccg gcagggcagg ctttcattaa tttacggaat tcattgatat
     4441 cgggatttgc cgccagaatg atatgcgggt ggccctcatc aacatggaat ttattgacgc
     4501 ctaatttgat gtcgacgtta accgtagcgt tctggctcat agcgcggttg ctcccttaat
     4561 gccatctttc agcaagttga tcagcccaat tttcttcgca tgtcccatga tcattttgcg
     4621 taccggctgg tttggtttgc cgtcaatggt gaacatctcg ttcatgatgt cggcgaccat
     4681 tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc gccgggatct tgcggaaatg
     4741 ctgcatatcg cgcatgatgc agctttgttc cagctcacgt ttgtattgcg ccagactgct
     4801 ggcggagaaa tcctcgcgtt ctttggcggc gatcaccgtt gtggcggcag cctgagccga
     4861 tgcaatggct aaatccatgc cacgaactgt aaaacccaaa ttcaggcaga agcctgcggc
     4921 gtcaccaacg atcatcacgc catcgttaac catctgcggc accattgcca gaccgccttc
     4981 tggcaccata tgcgcggaat attcaagcag tttgccgccg ctaattagtg ggcgaatggc
     5041 ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt ttttgcgcat gggcgatatc
     5101 acccaatcca caaaccagcc ccaaggatat ggaatccttg ttggtataga gaaatccccc
     5161 gcccatcagg ccgtcagaag gggaaccggc aaacagccag gcggcacctt cctcgcccgt
     5221 aatattaaag cgatcgttga tctgttctgg tgtgaggcca ataacctctt taacaccaac
     5281 agcgtaatga tgcggatcgg aagcgggaac cattcccagc gagcggccaa gcatcgagtt
     5341 aacgccatca gccagaatca ccacattcgc ttcgagaata tcatccccag cctgcacgcc
     5401 agtgaccttg tttccttcac gaaccagcgc atcgacgcga acgcctggga taaactgcgc
     5461 gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga cgattacgca atacggtata
     5521 agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa tcgagggtaa cggcgctttc
     5581 ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt tctaccggcg ctgatactgc
     5641 aaagcctgga atgattgctt caagtgtgtg ggcataaaga cgcccgccgg tcatgttttt
     5701 acatccggca ctgtcgccgc gttctatcac caggacatcc agcccggctc gcgccatgac
     5761 cagtgcggca acgctaccag caacgcccgc accgaccaca atggcatcaa atttgtcttc
     5821 cgacatgcgt tatgctccct tcccgctgcg tttacagcgg gagatgtggt tgaaagggga
     5881 attagcggct caactggcta atcagggcag ggacgacttt gtagatatcg cccaccagac
     5941 cgtagtcggc ataattgaag attggcgcat ttttatcttt attgatggcg gcaatcactt
     6001 ttgcgccgtt gccgccaacc atatgctgga tctgcccgga gatccccagc gtcaggtaga
     6061 gatcggattt cagcaacacg ccggagacac cgatataacg ttcacgctcc atccagttct
     6121 cgccttcggc aattggacgt gaacagccga cttcagcatt cagcaccgcc gccagttcgt
     6181 ggaccatttt caggtcatcc tgcgccgcca gaccacgacc gacgccaacc acacgttttg
     6241 ctttgctcag gtccacgctg cttgcggctt tcgcacggcg ttcgcgacag agaatttcat
     6301 gacgcggagc aacataaggt accgtttctg tcgggcactg atgagaggta tcactggtgc
     6361 acggttcctg aacaccgggt gcaagggtaa tgatcgccag cgggctgttg atcttttcct
     6421 gagcgaacgc taacccgcca tacatccggt gttcggcgca aatgtgacca tcgacaatat
     6481 ccaccgccgt ggcatcgttc accagcgccg cattcagttg cacacttaac cgtgctgcca
     6541 gagctttacc acgtttcgtc gcggccaaca gcaacatagc ggggtgttta tctttcagca
     6601 gggcagcaat gctttcggcg taattttcag tgcgttgcag cgcgtcgttt tgctcaagaa
     6661 cataaataca ttttggacca taaggcataa ctgcctgcgc ctggtcggta ttttgtacaa
     6721 tggcatacac ctgttggccc cattgctgag cgccgccaaa cagttcagca taacgttcag
     6781 gattatcgct aaagacccag acgctgttta attgactcat cgtttcttct ccgtaggctt
     6841 cagttcagag ctttcttcag atgttccgcc agctcggcaa tggcctccgg cgaatcgtta
     6901 tcgataatga tgtgcttacg ttctgtttgc ggcggtacgc gaatgccgac cagttcagca
     6961 agtggcgcgc tctggctcca gtcaatatca cttgcctgcc actgatttac cggtttttta
     7021 cccgcgccga gaatggcttt catcgaagga atgcgtggca cgttaatatc ggaggtgacg
     7081 cagagcacgg ctggaacaga gagttcaata acttcaacat catcttcaag cgtgcgttca
     7141 atcaccagtg tattgccctg acgctgaata gcactcactg cattaatcac cggaagttgc
     7201 agaatttctc cgaccagcaa gccaacctgc tgggcataaa ggtcgccgga accttcacca
     7261 aagatcagta aatcgaagcc gatcttttca attgctgccg ccagcgcttt tgcggtatcg
     7321 agaggcagtg catgttcaag ttgcgcatcc tgcaccaaat acaggctgtg cggcccgcgg
     7381 gatagcacgt ctttgcgcac tttcgagttc tgcaacaatg agccgccaac ggtcagcgcg
     7441 gctatctcat cgtcatctgt tgcgagctgg cttgcagctt caatggcatt gagatcgaac
     7501 tggctgattt tggcgtcggc attgtcgaaa ttcagggtgt attctggagt gacaacaatg
     7561 tcctgttctt caggcaccag cttaaagcag gttattattt tcatgatttc tcctgtgagt
     7621 cgcatacgga accatctccg atggaaggta aaaatggcct gaaaatagtg tgcgatagcg
     7681 gtgcggggtt ttcgacggag caagctgttt tccttgccaa caatcttgca aaaaaaatgc
     7741 gatcgctaaa acaaaagtgc aatttgcggc aatatagtta ttccatgacc gtacaatctt
     7801 gcattcagtg taaataatct tgtttagctc tcgctaaacg cggttgtata accgcaatac
     7861 acgccgtatt ttatccggca tatattgttt ttgcttgctc atggaatagg gaggttgtca
     7921 tgtatcaacg ctgttttgat aacgccagtg aaacgctgtt tgtcgccggt aaaacgccac
     7981 ggctttcacg ttttgcattt agcgatgatc caaaatggga gtccggacat cacgttcatg
     8041 acaatgaaac cgagttgatt tacgtcaaga aaggggttgc aaggtttacc atcgattctt
     8101 cgttatatgt cgcacatgcg gatgacattg tggtgataga acgcggcagg ctacatgcgg
     8161 tggcctctga cgttaacgat ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt
     8221 ttcagggggc tgaggaaaat cagctactgc aaccgcattc ttgtccggta attgccgcgg
     8281 ggcagggaaa agaagtcata aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa
     8341 gtaaaaatag ccaaacatct tcgttatggg atgcatttgc ctatacgtta gccattcttt
     8401 actacgaaaa ttttaaaaat gcttatcgtt cggagcaggg gtatattaaa aaagatgttc
     8461 tgataaaaga tattcttttc tatctgaata ataattatcg cgaaaaaatc actttagaac
     8521 agttatcgaa aaaatttcgt gccagcgtca gttatatttg ccatgaattt accaaagagt
     8581 atcgtatttc ccctattaac tatgttattc aacggcgtat gacggaagca aaatggtcac
     8641 tcactaatac tgaattatca caggcagaga tctcctggcg tgtgggttat gaaaatgtcg
     8701 atcactttgc caaactgttt ttgcgccatg tcggttgttc gcccagcgat taccgtagac
     8761 aatttaaaaa ctgttttgcg gaacaagaaa tcctatctga atttcctcaa ccggtaagtc
     8821 ttgttggata aatctggcgg cgcagtttgt taagaagctg cgccgcctgc agattatttg
     8881 ttctgatagt ctttcaggat ctgccgacct gctacgtaaa tcataatttc gtctgtaccg
     8941 ccgccgatac gttcacaacg gacatcacgc cagaagcggg agacgcgcgc ttcatcggta
     9001 tagcccagac cgcccatgat ttgaatcgca tcatcaatga cttccattgc ggtacgtgcg
     9061 caatacagtt ttgccagcgc cgcgctggtg cgcagtgact gatgctgatc ggcttgccat
     9121 gccactttca gcaccatgtt gcgcatgttg tcgatcttaa tcgccatcag cgccagtttt
     9181 tcctggatca tctggttatg accaatgggc ttaccaaaag cgatacgttg gttggcatag
     9241 cgggcggcat cttcaaaggc gcattcggca aagccggtgc tgcgcgcggc gttgatcagg
     9301 cgctccatct caaagttgta catcacattg aggaaaccca ttccttcttc gcccaccatg
     9361 tcgctctctt caacttccac attgtcgaga tagacttcgc aggtgctgag catatgccag
     9421 ccgattttat gcagcgggtt aatcttaatg ccgggcttac tggagtcgac ccaccacagg
     9481 gtgaaggctt ttttgggatc tttcggttgc ggatcgcgcg ccagcaccag catatacggg
     9541 tactctttcg cactggtaat aaaggttttt tgtccgttga tgtagacctt gccgttttta
     9601 cgcgtgtaag tggtagtggc actgttgtta tctgagccag cacctggttc cgtcaacgcc
     9661 agggcatagg cgggatcacc cgtttccagg gtgctctctg ccgttttacg tagctgctct
     9721 gcagaaccga aacggcgcat actgtgaata cattgaccgt tggtaattaa aaatgccgga
     9781 gcaccgcatt ttgacacttc catcagcgcc agcatttggg tgacgtaatc cgcagggata
     9841 ccaccaaatt cttccggcac gccaagcatg gaaataccgt tatccgccag cgcccgcata
     9901 aactcacgcg gatatgtccc gttttgatcg caggtgcgga aatactcttc cggaaagtta
     9961 gtcgtaatca gttcgcgaat actggccagc agcagttctt gttcttcagt taaagaaaaa
    10021 tccatcatta tactcctgtt aatgagccgc ttcaggcagg acaaaaccca tcgccgcatc
    10081 gataaataat ctttcgtcca tcagtttgag ttctggcgaa atcactgggg tgaagtccat
    10141 tttgtcgaga atatcttttt gtaaatcgac gccaggggcg atttcgatta aatgcaggcc
    10201 gtcttctttc agcgtgaata cagcacgttc ggtgatgtaa cgaacatcca gtccccgttc
    10261 gagcgcgatt tttccgctaa aagtaatttc cggtagttcc cgaataaatt tatttacccg
    10321 tccttcctgg acgatatgca atttgccatc ggcaatttct gtttttaaac tgcccgcagt
    10381 taacgtgccg cagaaaataa ttttcttcga ggtggcactg atatcaataa atccaccggt
    10441 acccatgatt ttgccattga atttatgcac gccgacgtta ccgtgctggt cgacttcagc
    10501 aaaactcaaa taacaaacat ccagaccacc accgtgataa aaatcaaact gggacgtcat
    10561 atccagaatg gcgcgggtat tcacgttcgc gccaaaggcg ataccctgtg aagtaattcc
    10621 gccaatcgga cctgtttcta ccgtcagaat aaagtcatca gcacaacctt cttcccgggc
    10681 gaccaggcca ataccgtcag caataccgac gccgacattc cccaccgcgc ctttacgcat
    10741 ttcgaataat gcacgccgcg caactaattt acgttggttt aggggcaggc taagtttgtg
    10801 ctgtcatcaa gggtgaagtc accagaaata aaacggttaa ccggcgcacc gccatacagt
    10861 tggctttgat ccggatcgac caccacaata tccaccagat aacccggaat acggacagat
    10921 ttaggatgca gcgtggcttt cttaaccatt ttctgcacct gcatcatcac aataccgccg
    10981 ttattgtgca ccgcctgggc aataaccaat gcgtcgagat acatcacctc atcttcaaaa
    11041 gtggcgtagc cttcactgtc gcaggtggta gcgcgaatga aggcgatatc tggcgcaatc
    11101 gctttgtaat agagatattc tttgttatca aactcgacca gtttaatcag gtcttctttc
    11161 gtgacttcat tcagtttgcc gccttgctgg cgtggatcga caaatgtccc gatgccaata
    11221 tcactaataa taccaggctg gtgggctgcg gcggcgcgta aggtttgtgt aagtacacct
    11281 tgtggatagt tataagcaat aattttattt tgttctgcga gttcagaaat acgcggcgat
    11341 tgtccccagt gaccacataa tgcccatttc accagacctt cttgcgccag aggactaata
    11401 ccacggtcgg cgcgatcgcc aagccctgtc ggactaataa tcgataaatt acgtggcgtt
    11461 tgagtctgtt tatatttatc agcaagagct gtaattaacg tggtggcttc cagaataccg
    11521 ccgccagcgc ctaacacaca aagtgttgct tcgtcgggaa tataattcac cgcttcctgt
    11581 gccgacagga ccggcactcg tccattaata cgaggtggtt ttacaggttt cattgttaat
    11641 tcctgattca gatgtgattt tccctacgcc agatggaga
//
LOCUS       DAZKGS010000134        11525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKGS010000134 DAZKGS010000000
VERSION     DAZKGS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            258..1646
                     /gene="narU"
                     /locus_tag="ACHOQY_003673"
     CDS             258..1646
                     /gene="narU"
                     /locus_tag="ACHOQY_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9679697.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1728..5468
                     /gene="narZ"
                     /locus_tag="ACHOQY_003674"
     CDS             1728..5468
                     /gene="narZ"
                     /locus_tag="ACHOQY_003674"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9679698.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5465..7009
                     /gene="narH"
                     /locus_tag="ACHOQY_003675"
     CDS             5465..7009
                     /gene="narH"
                     /locus_tag="ACHOQY_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9679699.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7009..7704
                     /gene="narW"
                     /locus_tag="ACHOQY_003676"
     CDS             7009..7704
                     /gene="narW"
                     /locus_tag="ACHOQY_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9679700.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7701..8381
                     /gene="narI"
                     /locus_tag="ACHOQY_003677"
     CDS             7701..8381
                     /gene="narI"
                     /locus_tag="ACHOQY_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9679701.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8460..9353
                     /gene="yddE"
                     /locus_tag="ACHOQY_003678"
     CDS             8460..9353
                     /gene="yddE"
                     /locus_tag="ACHOQY_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9679702.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9449..10294)
                     /gene="nhoA"
                     /locus_tag="ACHOQY_003679"
     CDS             complement(9449..10294)
                     /gene="nhoA"
                     /locus_tag="ACHOQY_003679"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP9679703.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10467..11036
                     /gene="yddH"
                     /locus_tag="ACHOQY_003680"
     CDS             10467..11036
                     /gene="yddH"
                     /locus_tag="ACHOQY_003680"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9679704.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11040..11267)
                     /gene="pptA"
                     /locus_tag="ACHOQY_003681"
                     /pseudo
     CDS             complement(11040..11267)
                     /gene="pptA"
                     /locus_tag="ACHOQY_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
     gene            complement(11366..>11525)
                     /locus_tag="ACHOQY_003682"
     CDS             complement(11366..>11525)
                     /locus_tag="ACHOQY_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9679705.1"
                     /translation="GNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLAS
                     VLAGRRLS"
BASE COUNT         2713 a         3000 c         3036 g         2776 t
ORIGIN      
        1 ttagattcac gatctgaagg gttgaaacat gacatacgtt caaagattaa cccccgtttg
       61 aaggttagct tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc
      121 ccccgcggtt acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta
      181 attctaataa ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg
      241 tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg
      301 actggaaacc agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa
      361 acctctggat atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg
      421 cagttaccgt taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat
      481 taaccgcatt accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc
      541 ctatattcgg tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg
      601 tctggctcgg aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg
      661 ctttgctatg cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct
      721 tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag
      781 gtgtaagtgt aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc
      841 tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat
      901 ggatttgggt accgctactg gcgattgcca cgatcgccgc atggtcaggg atgaatgata
      961 tcgccagttc acgcgcctca attgccgacc agctccctgt cttacaacgc ctgcatctct
     1021 ggctgctgag cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt
     1081 ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg
     1141 gcccatttat cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aagttcggcg
     1201 gcgtgcgggt gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc
     1261 ttaccttacc gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc
     1321 tgtttctgac tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc
     1381 gccagataac catttatcgg gtaaagatga aaggcggtag tgatgagcaa gctcagaaag
     1441 aagccgtcac cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg
     1501 gcttttttat tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg
     1561 cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg
     1621 gtcggcggaa gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc
     1681 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     1741 tggatcgctt tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg
     1801 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     1861 tcgtgcgttc cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta
     1921 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     1981 tgcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctttaca
     2041 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag
     2101 ccctcaagca acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa
     2161 agtgcctgag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     2221 aactaaacca gctgattgcc gccgctaacg tctggaccat caaaacctac ggcccggatc
     2281 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacgc
     2341 gttatctgtc gctgcttggc ggcacctgtt taagtttcta cgactggtat tgcgacctgc
     2401 cgcccgcctc gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt
     2461 ataactccag ctacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg
     2521 cccacttctt taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact
     2581 actctgaagt ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg
     2641 ccctggcgat ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg
     2701 actactttat caactactgc cgccgctaca gcgacatgcc gatgctggta atgctggagc
     2761 ctcgcgacga tggtagctac gttcccgggc gcatgatccg cgcatctgac ctggtggatg
     2821 gactgggcga aagcaacaat ccgcagtgga aaaccgtagc agttaatacc gcaggtgaat
     2881 tggtagtgcc gaacggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     2941 aatccattgc cgccggtacg gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     3001 ctgttgcagg cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg
     3061 taaaacacaa tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg
     3121 gcaacacctg tccggtggtc agcgtttatg atttggtact ggcgaattac ggcctcgatc
     3181 gcgggctgga agatgaaaac agtgcgaaag attacgctga aatcaaaccg tacaccccag
     3241 cctggggtga gcaaattacc ggcgtgccgc gccagtatat tgaaaccatc gcccgtgaat
     3301 ttgccgatac cgcccataaa acgcatgggc gctcgatgat tatcctcggc gcaggtgtta
     3361 accactggta tcacatggac atgaactacc gcgggatgat caatatgctg atcttctgcg
     3421 gttgtgtcgg gcaaagcggt ggcggctggg cacactatgt cggccaggaa aaactgcgcc
     3481 cacaaaccgg ctggttgccg ctggcctttg cgctcgactg gaaccgacca ccacgccaga
     3541 tgaacagtac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     3601 ctcaggagtt actttcaccg ctcgccgatg ccagtaagta cagcggtcat ctgattgatt
     3661 tcaacgttcg cgccgaacgt atgggctggc taccttctgc gccgcagctg gggcgtaacc
     3721 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     3781 aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc
     3841 atccgcgtaa tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg
     3901 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     3961 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     4021 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     4081 ttctgcccac cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt
     4141 ttattcatcc gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa
     4201 tctacaaagg tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa
     4261 ccgacgtggt attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg
     4321 aagtgctcga ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata
     4381 ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat
     4441 tgatggacaa acttggcaat ggcggtaaag ggatttcgtg gaatacgcag gatgaaatcg
     4501 atttcctcgg caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga
     4561 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc
     4621 atgttgcagt caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc
     4681 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     4741 aaattatctc cagccccacc tggtccggtc tggaaagcga tcacgtatcc tataacgcgg
     4801 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct
     4861 atcaggatca tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta
     4921 tcgacacccg tagcgtcagt gagatgcgcc agataccgcc aaacggcttc ccggaaaaag
     4981 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     5041 tgctaatgct gacgctctct cgcggtggac cgattgtctg gatcagcgaa acagatgccc
     5101 gagaactgac cattgtcgat aacgactggg tggaagtatt caacgccaac ggcgcgctga
     5161 ctgcccgcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     5221 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     5281 actcggtcac ccgcgtttgc ccgaaaccaa cgcatatgat tggcggttac gcgcagctgg
     5341 cctggggctt taactactac ggcaccgtcg gatcgaaccg cgatgagttc atcatgatcc
     5401 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     5461 aaaaatgaaa atccgttcac aagtcggcat ggtgcttaac ctcgacaaat gtatcggctg
     5521 ccatacctgt tcggtgacct gtaaaaacgt ctggaccggg cgcgaaggca tggagtacgc
     5581 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     5641 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     5701 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga
     5761 ttactacgaa cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca
     5821 tatccctact gctcgcccgc gttcactgat tgacggcaaa cggatggaca aagtgatctg
     5881 gggcccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
     5941 cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct
     6001 gccgcgcctg tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc
     6061 tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg
     6121 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
     6181 gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc
     6241 agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat
     6301 tgaggaagcg gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtatt
     6361 cctcgatcca cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa
     6421 cgtgattgaa gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc
     6481 gctaccgctg caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc
     6541 gccgattcag tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc
     6601 catcgaaagc ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac
     6661 cggtccggta ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca
     6721 aaccgtggaa ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca
     6781 ggtcgaagag atgtatcgtt acctcgccat tgccaactat gaagatcgtt ttgtcatccc
     6841 gacgagccat cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac
     6901 ctttggcgac ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat
     6961 cgatgccatc aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc
     7021 aaagtgatcg gcctgttgat ggagtatccg gacgagctgt tgtgggaatg caaggaggac
     7081 gcgctggcgt tgatccgcag cgacgcgccg atgcttacgg atttcaccca caacctgctt
     7141 aacgcgccgc tgctggataa acaggccgaa tggtgcgaag tgtttgaccg tggacgtacc
     7201 acatcgctgc tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg
     7261 gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc
     7321 gattatctac cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa
     7381 ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag
     7441 gcaccgtggt acgcgttgtt tgatgctctg ctgcaactgg cgggaagctc cctttcaagt
     7501 gacagtgtca caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat
     7561 gcagtgtggg aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc
     7621 tcgccgctta atcaatatca gcgacgcttt agccaggatg tcgcgccgca atatgtcgac
     7681 atcagtgcgg gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc
     7741 cgtacatttg tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt
     7801 acacctggcg cgcctcctca agtcaaatgc tcgataaacg cgagatggtg atatggtcga
     7861 atctgttcca tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc
     7921 cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactaatg gcgatggtgc
     7981 tcggcggtat ttgcggtgtg ttgacattga ttggcggcgc aggactgctg tggcgcaggc
     8041 tgacaaatca gcgcgtgcgt gcaacttcca ctacgccgga tattatcatc atgagcattt
     8101 tgctgatcca gtgtctgctg gggctaagta ccataccgtt ttcagcacag tatcctgatg
     8161 gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat
     8221 cgtcagaaat gcttaacggc gtagcgttcg ttttccgcct gcatctggta ttgggaatga
     8281 ccatcttcct gctcttcccg ttcaccagat tggtgcatgt gtggagcgcg ccgtttgagt
     8341 actttactcg tcggtatcag attgtgcgtt cgcggcgata atttcaccct ggccggatag
     8401 cccttcgctc tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa
     8461 tgaaaccgca ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg
     8521 ccggtgttgt tttccccgcc gataatctta gcgaagcgca aatgcagctt atcgcccgcg
     8581 agttaggcca ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc
     8641 gctactttac gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact
     8701 atgtacgtgc gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag
     8761 gaaaacatcg cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag
     8821 gtacgccggg ctttgagcca ccgctggaag gtgaaacacg tgaggcgatt atcaacgcgc
     8881 ttcatctcac tgaagacgat attttgccgg gcttgccaat tcaggtcgca acaacaggac
     8941 actcaaaagt gatgatcccg ttgaaaccgg aagtggatat cgacgccctt tcgcctgatc
     9001 ttgctgcgct gagcgctatc agcaaacaga ttggctgcaa tggtttcttc ccgttccaga
     9061 tccgcccagg caaaaacgaa accgatggtc gtatgttctc gcctgcgatt ggtattgtgg
     9121 aagatccggt gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg
     9181 tattgcccca cgatggcaac gtgttatgtg ttaaaggcca tcaggggcgc gcattggggc
     9241 gtgacggcat gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca
     9301 tttcaggcac agcggtgatt ttgtttcatg ccgaatgggc aattgaactc tgaaaccgtt
     9361 gcctgatgcg cttcgcctgt caggtctaca accgagctaa tgtatgccgg ataaggcgtt
     9421 tacgccgcat ccggcaacct gacataaatt attttcccgc ctccgggtgc gtatcaaacg
     9481 ccgccatcac cagcgctaac tcatccacgg taaagccatg tttcgcatca tccacgccca
     9541 gaccaaactg ttcttgcatc acagcatata atgacgccac atccggtaga tttcgctgct
     9601 ccaccgcgtg cccattttca taatgggtaa aatgaaaatt ggtcagcgtt agcttgccac
     9661 cgtccggcaa atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg
     9721 ccgaccagaa attgcccatc acataatcgc tttgttgctg ctcgcagaga tcaaaacggt
     9781 acatcgactg ccagtgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca
     9841 acaaccgata atctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg
     9901 cggttagcgt ctgcccaccg aaaccgacat cagcaatcca tttttcctct tccagttcca
     9961 ccaacagcaa acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc
    10021 ctaacaagct gcgaacgtta aactccagct cgcgtaacac ccgctcaaac acgccattct
    10081 gctcaaaaca gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat
    10141 caagctgtat ttccctcggc agcaaaacgt cgaggttttc aaacggaata gtgcaattgt
    10201 gtttcaggtg caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa
    10261 tacgggcaaa atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc
    10321 aacttataac ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca
    10381 cgtttgcaaa tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa
    10441 aaaaaccttc ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg
    10501 ccagccgtct gttaaatcat ggcccaactg tcatgatcac cagtttcgat gagcaatccc
    10561 agcggcgtaa cattatggca gcagcctggt caatgccggt ggagtttgaa ccgccacgcg
    10621 tggcgattgt ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaaat
    10681 ttggcatcgt tatcccgggc gttgcagcaa ctaactggac gtgggcagtg ggaagcgtgt
    10741 cgggtcgtga agaagataaa tttaattgct atggcattcc ggtggtgaaa ggtccggtgc
    10801 ttggtttgcc acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg
    10861 cgacttctgc gcaacaaaaa tacgacacgc tgtttggtga ggtggtatca gcagcggcag
    10921 acgcacgggt atttgtcgaa ggccgctggc agtttgatga tgataagctc aatacgttgc
    10981 atcatttagg tgctggcacg tttgttacca gcggcaagcg agttacggcg ggttaagcat
    11041 tacatgctgt aaccgggttt ctttatcaaa gcctccattt ggggcgcgat ttcggcatcc
    11101 cagatagctt gccaagattc tggttgaatc tgctgtagag caatgcttat cgagctatct
    11161 ttactgttca gatggcgaat aataacgtcg gtaatatctg cagcaagtgc tgctttttat
    11221 tgttcgtcca gttcacgcgg aaaacattta atgtcgatgt gcggcatgat tgtgctcctt
    11281 ttgaaagatg tcagtaaaat taacacacat tgtgaatgtc gtgtgtccgg taatgcgttt
    11341 ttacgacaat aatgccaggg caagattacg aaagccttct ccccgcaagg actgacgcca
    11401 ggtagtttct gtccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11461 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11521 ttcct
//
LOCUS       DAZKGS010000135        11490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKGS010000135 DAZKGS010000000
VERSION     DAZKGS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            200..817
                     /gene="yijF"
                     /locus_tag="ACHOQY_003683"
     CDS             200..817
                     /gene="yijF"
                     /locus_tag="ACHOQY_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9679706.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(844..1749)
                     /gene="yijE"
                     /locus_tag="ACHOQY_003684"
     CDS             complement(844..1749)
                     /gene="yijE"
                     /locus_tag="ACHOQY_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9679707.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1843..4023)
                     /gene="katG"
                     /locus_tag="ACHOQY_003685"
     CDS             complement(1843..4023)
                     /gene="katG"
                     /locus_tag="ACHOQY_003685"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9679708.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4352..5242)
                     /gene="metF"
                     /locus_tag="ACHOQY_003686"
     CDS             complement(4352..5242)
                     /gene="metF"
                     /locus_tag="ACHOQY_003686"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9679709.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5318..5452
                     /locus_tag="ACHOQY_003687"
     CDS             5318..5452
                     /locus_tag="ACHOQY_003687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679710.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5591..8023)
                     /gene="metL"
                     /locus_tag="ACHOQY_003688"
     CDS             complement(5591..8023)
                     /gene="metL"
                     /locus_tag="ACHOQY_003688"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9679711.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(8026..9186)
                     /gene="metB"
                     /locus_tag="ACHOQY_003689"
     CDS             complement(8026..9186)
                     /gene="metB"
                     /locus_tag="ACHOQY_003689"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9679712.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9463..9780
                     /gene="metJ"
                     /locus_tag="ACHOQY_003690"
     CDS             9463..9780
                     /gene="metJ"
                     /locus_tag="ACHOQY_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9679713.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9964..10572
                     /gene="yiiX"
                     /locus_tag="ACHOQY_003691"
     CDS             9964..10572
                     /gene="yiiX"
                     /locus_tag="ACHOQY_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9679714.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10717..11392
                     /locus_tag="ACHOQY_003692"
                     /pseudo
     CDS             10717..11392
                     /locus_tag="ACHOQY_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2818 a         3201 c         2892 g         2579 t
ORIGIN      
        1 aataaacttc aatatattga atttgcatga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcac tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg
      121 cttctggaca ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt
      181 attttcacgg acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt
      241 taccagccac tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa
      301 taccaacctt gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga
      361 tccggcgtat atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg
      421 ttccgatgtg gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca
      481 cgaagatatg gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga
      541 cagcaacatc gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa
      601 aacacgcccc atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg
      661 actggataac ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac
      721 gccgctggtg atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg
      781 gcggatggtg gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa
      841 aattcagatg ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc
      901 cagcacaatc agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca
      961 ccaggaaaag agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc
     1021 aggcaagttt ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc
     1081 actgtaggcc agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag
     1141 taaagcgacc acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa
     1201 taaatccacg cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca
     1261 actgacgccg gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca
     1321 cggctgcaac accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg
     1381 tcgcaggcgt tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata
     1441 gcttaggatc gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac
     1501 catcccgcag gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat
     1561 tccgcgacca cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa
     1621 ggcggtaaag tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata
     1681 actccagata agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc
     1741 gacagacatg agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa
     1801 cttcagcgac tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg
     1861 gtcgaggttc atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc
     1921 atcgctgctg gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag
     1981 atccgcacgg ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag
     2041 ctctttcgat tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa
     2101 gtcattgctc aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt
     2161 ggcacccagt acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag
     2221 ttgctgggct ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata
     2281 gttacggaaa ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc
     2341 ctgacgcgca tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct
     2401 tgcggctttc tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt
     2461 accagactct ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac
     2521 atcccagtcg cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc
     2581 gccaccacgg aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag
     2641 accagaatcc gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat
     2701 cggctgcggc agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta
     2761 gcgagatttc ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc
     2821 gttgaacgcc tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa
     2881 acgcagcgtc aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg
     2941 atccgggata atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg
     3001 ggtctgtacc cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg
     3061 cgtctgggtc cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc
     3121 gtaagtgctc gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc
     3181 tacatttgat gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag
     3241 cgccacggtt tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc
     3301 cgcagaaagc ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat
     3361 ctcggttgca cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc
     3421 tttttcatca ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc
     3481 aaaaccgaag gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag
     3541 gtcggcccag gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc
     3601 tttatcgagg cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg
     3661 acccgcgccg ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg
     3721 aataaacaga ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag
     3781 cagggctttc agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta
     3841 gtcaaagtcc tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg
     3901 gagttgattt ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc
     3961 gccctgatgg aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct
     4021 catcaatgtg ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta
     4081 taattgaatt aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat
     4141 gtagatcaaa ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt
     4201 tataaaaaag ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca
     4261 accttatttt tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat
     4321 cacagtgtga ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat
     4381 ggcaaatcgc gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc
     4441 cttcacggct taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg
     4501 tttcagcatc gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt
     4561 tggtcatatc ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa
     4621 taatttccac atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga
     4681 catcaaagaa gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt
     4741 tgagcaaatc cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg
     4801 agatatcgaa atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg
     4861 gcttaccact tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat
     4921 tccagtagtc gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat
     4981 gcggtgccgc ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt
     5041 cgcgctcgcc ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa
     5101 ggcgatcgat ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact
     5161 cgaacgaaac gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct
     5221 ggctggcgtg aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt
     5281 tgtgcacatc tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt
     5341 caaccgaaaa atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga
     5401 tggtaagaaa aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca
     5461 tgtacttatt gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat
     5521 aattcaatat atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa
     5581 atttctgaaa ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg
     5641 tgacgtcgcg cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc
     5701 tttcgatggc aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca
     5761 ccgcttccac gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca
     5821 ccagccccat ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc
     5881 cattttcaaa gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca
     5941 cacgtacctg gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc
     6001 gcattacgtc tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc
     6061 acgcctgatc caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg
     6121 agagcgtgcc ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat
     6181 cgcgcacggt gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac
     6241 gcccggtttt ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg
     6301 ccagtttgtt ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag
     6361 caagctgctg gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca
     6421 tccacaggaa caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg
     6481 cgcggctggc gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg
     6541 ccagcacaaa ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt
     6601 ccagccaacg ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg
     6661 cgcggaagac ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta
     6721 ctgccactag gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca
     6781 gttgctgcca gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg
     6841 ccaccagcgc cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga
     6901 ttttcagcgc actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt
     6961 tatgtacgcc aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt
     7021 tatgcgccag tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat
     7081 cgtggctggt gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg
     7141 aaccttgatc cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg
     7201 gctgtaaagt acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat
     7261 ccagacgcag caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata
     7321 ccccggcgac gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg
     7381 tcgcggaata gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc
     7441 tgataaatcc ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca
     7501 acgggtaaga aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact
     7561 cgcgggcatc aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc
     7621 gtgccgacca tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc
     7681 tgtcgagcag cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg
     7741 cttcttcagc gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt
     7801 gaacctgatg cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt
     7861 tagtggtgct accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca
     7921 taatgcccgc gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat
     7981 gcagctgacg acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc
     8041 agcccggaag ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga
     8101 gatacgcagc agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc
     8161 atgtgtcatg gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag
     8221 cgtaaacaac gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc
     8281 aaaactcaac attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt
     8341 ttccggcagc gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac
     8401 aatcgcctgc gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg
     8461 tagcagcaga tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc
     8521 aagttcagtg acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg
     8581 accgttcaga tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt
     8641 ttgtaatgcc gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc
     8701 cagatggcag attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac
     8761 cagtaccagt ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc
     8821 aacaaacaac acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc
     8881 gccgtagcag tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac
     8941 caggtgaatc gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc
     9001 tgccagcgca cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc
     9061 gcgcggttca ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac
     9121 gcaaccatac tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg
     9181 cgtcatgtga tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat
     9241 tgtgttaatg gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa
     9301 atcccaaata gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt
     9361 cacggtaacg cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg
     9421 tggtctggtc tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc
     9481 gaatatatca gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg
     9541 gtttccattc ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg
     9601 aacaacctgc gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt
     9661 accgggcaac ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg
     9721 gaagcggcaa aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa
     9781 agcggaaatg agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt
     9841 catattgcaa tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca
     9901 tccggcataa acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta
     9961 actatgaaaa acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg
    10021 cagccgcaaa ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc
    10081 caactggcaa cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag
    10141 ccctacattt ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc
    10201 catggtgaaa agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa
    10261 cagcaaaaac tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc
    10321 tcatggagcg acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg
    10381 ctgggaatgc gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg
    10441 gtccaggcga agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt
    10501 tcaccacaag ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg
    10561 ttttcgtggt aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt
    10621 agttgcacga cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg
    10681 aattaaggat ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc
    10741 ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga
    10801 ttccgcggcc gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa
    10861 ccgggagtta aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact
    10921 gcccgtacgc tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac
    10981 gaagaataac gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc
    11041 agcaatggag ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca
    11101 cgaaaactgg tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg
    11161 aattgcgggc gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag
    11221 ttacgctaga gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat
    11281 tgagcgtaac aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg
    11341 tttctcgcgt tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg
    11401 ttctactaat tggagtcatc acccttctga taagctttcc ttggatgatt accagactga
    11461 atacacagcc aaagattttt acctcattct
//
LOCUS       DAZKGS010000136        11311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKGS010000136 DAZKGS010000000
VERSION     DAZKGS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..151
                     /locus_tag="ACHOQY_003693"
     CDS             17..151
                     /locus_tag="ACHOQY_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9679715.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            130..234
                     /gene="speFL"
                     /locus_tag="ACHOQY_003694"
     CDS             130..234
                     /gene="speFL"
                     /locus_tag="ACHOQY_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP9679716.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            545..2743
                     /gene="speF"
                     /locus_tag="ACHOQY_003695"
     CDS             545..2743
                     /gene="speF"
                     /locus_tag="ACHOQY_003695"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9679717.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2740..4059
                     /gene="potE"
                     /locus_tag="ACHOQY_003696"
     CDS             2740..4059
                     /gene="potE"
                     /locus_tag="ACHOQY_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9679718.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4115..5755)
                     /gene="pgm"
                     /locus_tag="ACHOQY_003697"
     CDS             complement(4115..5755)
                     /gene="pgm"
                     /locus_tag="ACHOQY_003697"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9679719.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5781..6326)
                     /gene="seqA"
                     /locus_tag="ACHOQY_003698"
     CDS             complement(5781..6326)
                     /gene="seqA"
                     /locus_tag="ACHOQY_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9679720.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6511..7275
                     /gene="ybfF"
                     /locus_tag="ACHOQY_003699"
     CDS             6511..7275
                     /gene="ybfF"
                     /locus_tag="ACHOQY_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9679721.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7415..7708
                     /gene="ybfE"
                     /locus_tag="ACHOQY_003700"
     CDS             7415..7708
                     /gene="ybfE"
                     /locus_tag="ACHOQY_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9679722.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7848..8378
                     /gene="fldA"
                     /locus_tag="ACHOQY_003701"
     CDS             7848..8378
                     /gene="fldA"
                     /locus_tag="ACHOQY_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9679723.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8588..8674
                     /gene="uof"
                     /locus_tag="ACHOQY_003702"
     CDS             8588..8674
                     /gene="uof"
                     /locus_tag="ACHOQY_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9679724.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8667..9113
                     /gene="fur"
                     /locus_tag="ACHOQY_003703"
     CDS             8667..9113
                     /gene="fur"
                     /locus_tag="ACHOQY_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9679725.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9197..9523)
                     /gene="chiQ"
                     /locus_tag="ACHOQY_003704"
     CDS             complement(9197..9523)
                     /gene="chiQ"
                     /locus_tag="ACHOQY_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9679726.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9573..10979)
                     /gene="chiP"
                     /locus_tag="ACHOQY_003705"
     CDS             complement(9573..10979)
                     /gene="chiP"
                     /locus_tag="ACHOQY_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP9679727.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2745 a         2768 c         2822 g         2976 t
ORIGIN      
        1 gtgctattca taattaatga tttattattt ggggtatgat cgttttttgt tgatcttctt
       61 cacagattat agccatttca tggatagaat aactctacct tcaactgaca cagcaagagg
      121 taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc
      181 atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct
      241 gactaaaggg caccccaacg tacaggtctc cctgacttta agcattacag gttaatacct
      301 gtattcctcg gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag
      361 cacttgcaac ttgaggtatg acgagtatag ccagttaccg ggctggtctg ggttattgca
      421 tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc
      481 gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
      541 caaaatgtca gaattaaaaa ttgccgttag tcgttcttgc ccggattgtt tttccactca
      601 tcgtgcatgc gtgaatatag acgaaagtaa ttatattgac gttgccgcca ttattttatc
      661 agtcagtgat gttgaacgtg gaaaactcga tgaaatagac gctactggct atgacattcc
      721 tgtttttatt gcaacggaaa atgaagaacg tgtacccgca gaatatctgc cacgtatttc
      781 tggtgtcttc gaacattgcg aatcacgtaa agagttttat ggacgtcagt tagaaaccgc
      841 tgccagccat tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt
      901 taatcagggc aatagtgctt ttgactgccc tggtcatcag gggggtgagt ttttccgtcg
      961 ccatccggca ggcaatcagt tcgtggaata ctttggcgaa atgctcttcc gctctgactt
     1021 gtgtaatgct gacgtagcaa tgggcgatct gctgattcat gaaggtgcgc catgtattgc
     1081 acaacagcat gcggctaaag tctttaatgc cgataaaacc tatttcgttt taaacggtac
     1141 ttcatcttct aataaagtgg tcttaaacgc attgctgacg ccgggcgatc tggtgttgtt
     1201 tgaccgcaat aaccataaat ctaaccacca cggtgcgttg ctacaggctg gcgcgacacc
     1261 ggtttatctg gaaaccgcac gtaacccgta tggctttatc ggtggcattg atgcgcactg
     1321 ttttgaagaa agttacctgc gtgagctgat cgcggaagtc gcaccgcagc gggcaaaaga
     1381 ggctcgtcct ttccgcctcg ctgtgattca gttaggcacc tacgacggta cgatttataa
     1441 cgcccgccag gtggtggata aaattggtca tctgtgtgac tacattctgt ttgactccgc
     1501 atgggttggc tatgagcaat ttatcccgat gatggctgac tgttcgccgc tgttgctgga
     1561 tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg
     1621 tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta
     1681 tgtaccgcac aaacgcatga acaacgcctt tatgatgcat gcctccacca gcccgttcta
     1741 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     1801 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aagctgatcc tcgataactg
     1861 tcagcatatt cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
     1921 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccaggtg agcactggca
     1981 ctcttttgaa ggctatgcag agaatcaata ctttgtcgat ccatgcaaac tgttgctgac
     2041 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
     2101 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2161 cctcttcctg ctgactccgg cagaagatat ggccaagctt cagcaacttg ttgccctgct
     2221 ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat
     2281 ctacaaacag catgaagagc gctacgccgg ttataccctg cgtcagttgt gtcaagaaat
     2341 gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     2401 acacttccca cgcgtaagca tgaatccgca agaagccaac tacgcctatt tacgcggtga
     2461 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     2521 ttatcctccg ggtgtgctgt gtgttgttcc gggtgaaatc tggggtggtg ctgttttgcg
     2581 ttacttcagc gctctggaag aagggatcaa cctgctgcca ggttttgcac cggagctgca
     2641 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2701 tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac
     2761 aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     2821 atcatgctgc cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg
     2881 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     2941 aaatcaggcg gtatgggcgg ttatgcggag tatgctttcg gtaaatccgg taactttatg
     3001 gcgaactata cctacggcgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     3061 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3121 accatcgggg tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3181 caaatcagta gcattaccgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     3241 ggctggttct ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg
     3301 ttcttcagtg cagtaggttc ttccatcgct atgacgctgt gggcttttct cggtctggag
     3361 tctgcgtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     3421 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg
     3481 attgtgccta atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag
     3541 atgttcacac cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3601 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc
     3661 tacttcccta aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg
     3721 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     3781 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     3841 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     3901 gcaaactttg ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg
     3961 gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg
     4021 gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc
     4081 gatcttgcga ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa
     4141 caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc
     4201 agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag
     4261 tcatcacttt cagaccgccg atagaagcac cattacccgg tgccgccgtc agacgtgcgg
     4321 tgatcgggtc acctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacaacg
     4381 ccgctttttg tgcggaagtc gcagcggcct gcaaacggtt gtagctcggc gcaccaaagc
     4441 gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg
     4501 ccgccagcag acacataatg atgccgtctt tatcggtgga ccacggcgtg ccgtcgaaac
     4561 gcaggaagga agccccggcg ctctcttcgc cgccaaagcc gaaactgccg tcgaacagac
     4621 catcaacaaa ccatttgaat cctaccggga cttctaccag tttacggccc aaggcgttga
     4681 ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc
     4741 actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca
     4801 tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca
     4861 gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag gagcagtcca
     4921 tacggatcgt gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt
     4981 taacgatggt caggttgagg ttgtaatact cgccaatacg cttccagtat tcgataccgg
     5041 aaccgcccag cggatcaacg ccaagcgtca ggcccgcttt ctgaatcgcc gccatatcaa
     5101 cgatatcggc cagaccttcc acaaacggct gcaccagatc ctgctctttc acatgaccgg
     5161 atgccatcgc ttcgtcgaga gagatacgct tcacgccttt caggccatcg gccatcagtg
     5221 cgttggccct gtcttccacc actttggtga cgttggtatc agccgggcca ccatttggcg
     5281 ggttgtactt gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt
     5341 ctgccagcgg gccacctttt ttattgtgaa ccaggatggc attggaaacg gcaggcgtcg
     5401 gagtgaatcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca
     5461 gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc
     5521 cagtgatgcc atttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg
     5581 gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg
     5641 cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga
     5701 cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt
     5761 tgtcctttgt ctgcaacgtt ttagatagtt ccgcgaacct tctcaatcaa ttccgccggg
     5821 aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg
     5881 gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc
     5941 agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt
     6001 gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga
     6061 ttgaccgctc gcttttgctc tgcgtattcg tccgaaagca gaagttcacg cattgcgcga
     6121 accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga
     6181 acctctttcg tcaccggagc agcaggctgt gatgaggcgg aaaatttcaa catacgccgt
     6241 aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt
     6301 tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag
     6361 tcgccaggag tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa
     6421 aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta
     6481 acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca
     6541 caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac
     6601 aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg
     6661 cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat
     6721 cttgttgata ctctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg
     6781 ggcggtaaag cggtaatggc acttactgca ctggcccccg atcgcatcga taaactggtg
     6841 gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct
     6901 atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc
     6961 cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag
     7021 tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa
     7081 atcccggcat gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc
     7141 gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca
     7201 ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc
     7261 tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg
     7321 ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc
     7381 ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac
     7441 gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc
     7501 gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg
     7561 ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc
     7621 ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc
     7681 ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc
     7741 ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg
     7801 caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg
     7861 gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac
     7921 agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg
     7981 aagcttatga cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg
     8041 actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc
     8101 tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca
     8161 tccgcgacat cattgaaccg cgcggtgcaa ccatcgttgg tcactggcca actgcgggct
     8221 atcatttcga agcatcaaaa ggtctggcag atgacgacca ctttgtcggt ctggctatcg
     8281 acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt
     8341 ctgaagagtt gcatctcgac gaaattctta atgcctgatg tgatgcggcg tagactcatg
     8401 tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg
     8461 ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg
     8521 tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa
     8581 ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa
     8641 ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg
     8701 gcctgaaagt aacgcttcca cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc
     8761 atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc
     8821 tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca
     8881 attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga
     8941 tttgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc
     9001 gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc
     9061 actgtgccga aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc
     9121 tgaacgagaa aagccaacct gcgggttggc ttttttatac aagggaaata agaaaagcga
     9181 gagttacagc tctcacttat ttgttattac tgcgtatttc ctgccagacc ttatcacaat
     9241 cggctttcac cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca
     9301 gtacgcgtac actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt
     9361 ttaacacgct ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg
     9421 cgctgtatgc ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta
     9481 ccagccccga tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg
     9541 tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc
     9601 ataaatttca cgtcacgctc atcctggaag atgttaccgt aaccgccgcc ccagcttggg
     9661 atgtcagagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga
     9721 ccgtcctgaa tggtgtagac cgcatccagg ctgtaggcag actcttcaat agtccggttt
     9781 ttgtcgtagt acgcatccgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa
     9841 acgtaggaag cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca
     9901 ccgaagaaga ccgctttttc gccgttggcg ttgaagtcgg aacggttatc ccaccagata
     9961 tccaggcggc cgttggagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc
    10021 tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg
    10081 taaccaaagg tcaacgcttg cagccaggcg gtgccgtcat aaaggtcgtt gacgctgcga
    10141 tcgtcaactt tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg
    10201 atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca
    10261 aatgccgctt ccagtacgaa gttatttttg aagtcgtatt tcgcaccgat ggagtgcagg
    10321 taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc
    10381 gctttgtatt cgttggtcca catgtaggag aaactcaagg caccagcatc gccgtaatca
    10441 aaattcgccc cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct
    10501 aacagcgttt gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta
    10561 aatttggccg cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca
    10621 taggctttat tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc
    10681 gccatttcaa tcgccgtaaa cgcagcaata tcaaggccga acatatcagc agcataaccg
    10741 gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg
    10801 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag
    10861 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
    10921 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
    10981 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa
    11041 ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac
    11101 gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt
    11161 tgatgatttt tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagc
    11221 gtgaatttcc agcaagatga aagcggttac attttattta attatggttt gtgattttaa
    11281 atttatttta tggataaatt gcgaagcgga a
//
LOCUS       DAZKGS010000137        11266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKGS010000137 DAZKGS010000000
VERSION     DAZKGS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1105
                     /locus_tag="ACHOQY_003706"
                     /pseudo
     CDS             1..1105
                     /locus_tag="ACHOQY_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            1441..2418
                     /gene="glaH"
                     /locus_tag="ACHOQY_003707"
     CDS             1441..2418
                     /gene="glaH"
                     /locus_tag="ACHOQY_003707"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9679728.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2438..3706
                     /gene="lhgO"
                     /locus_tag="ACHOQY_003708"
     CDS             2438..3706
                     /gene="lhgO"
                     /locus_tag="ACHOQY_003708"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9679729.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3729..5177
                     /gene="gabD"
                     /locus_tag="ACHOQY_003709"
     CDS             3729..5177
                     /gene="gabD"
                     /locus_tag="ACHOQY_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9679730.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5191..6471
                     /gene="gabT"
                     /locus_tag="ACHOQY_003710"
     CDS             5191..6471
                     /gene="gabT"
                     /locus_tag="ACHOQY_003710"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9679731.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6782..8182
                     /gene="gabP"
                     /locus_tag="ACHOQY_003711"
     CDS             6782..8182
                     /gene="gabP"
                     /locus_tag="ACHOQY_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9679732.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8203..8865
                     /gene="csiR"
                     /locus_tag="ACHOQY_003712"
     CDS             8203..8865
                     /gene="csiR"
                     /locus_tag="ACHOQY_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9679733.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8866..9315)
                     /gene="kbp"
                     /locus_tag="ACHOQY_003713"
     CDS             complement(8866..9315)
                     /gene="kbp"
                     /locus_tag="ACHOQY_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9679734.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9399..9557)
                     /gene="yqaE"
                     /locus_tag="ACHOQY_003714"
     CDS             complement(9399..9557)
                     /gene="yqaE"
                     /locus_tag="ACHOQY_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9679735.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9740..10039
                     /gene="ygaV"
                     /locus_tag="ACHOQY_003715"
     CDS             9740..10039
                     /gene="ygaV"
                     /locus_tag="ACHOQY_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9679736.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10049..10573
                     /gene="ygaP"
                     /locus_tag="ACHOQY_003716"
     CDS             10049..10573
                     /gene="ygaP"
                     /locus_tag="ACHOQY_003716"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9679737.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(10620..11024)
                     /gene="stpA"
                     /locus_tag="ACHOQY_003717"
     CDS             complement(10620..11024)
                     /gene="stpA"
                     /locus_tag="ACHOQY_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9679738.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2742 a         2805 c         2951 g         2768 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacctggaat atacaaaaac atggggagta atagcgttaa
      961 cattattatt aataataccc gaaaaattat cccccctggt aaagtattta cgcttagagg
     1021 cggaactcta aatatcaata ttcctgggcg tagcgctctt cttttaggga aaacaggaga
     1081 accgccgaac tatctctatt tataatttaa tgttatatct gccccgataa aacggggcag
     1141 ataatatgtt tagtttacta acggtcattt tgcagtgaag ccatttactg ttttttatcg
     1201 accagataat ctgttctcta atgttaactc cccctaacct gttgctttag ttattcattt
     1261 cctgtctcac tttgccttaa taccctacgt taaatgttac taatttgttg cttttgatca
     1321 caataagaaa acaatatgtc gcttttgtgc gcatttttca gaaatgtaga tatttttaga
     1381 ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg
     1441 atgaatgcac tgaccgccgt acacaataac gctgtcgatt caggccagga ctatagcgga
     1501 ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag
     1561 acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa
     1621 tcgtttctgc gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa tcagctgcaa
     1681 ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg
     1741 ggtatcgatg atgtggcgca ggcagatgag atggtgaagc tggcaacggc ggtagcgcat
     1801 ctgattggtc gctcaaattt cgatgcgatg agcggtcagt attacgcgcg tttcgtggtg
     1861 aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtgat ggaactgcac
     1921 aacgacggca cgtatgtcga agagatcacc gattacgtgc tgatgatgaa aatcgatgag
     1981 caaaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac
     2041 cactatttcc gccatccact ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa
     2101 aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg
     2161 atgcgctata tcgaccagtt cgtccagcca aaagattttg aagaaggcgt gtggttgagc
     2221 gagctttccg atgccattga aaccagcaaa ggcattcttt ctgtacccgt tcccgttggc
     2281 aaattcctgt tgataaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac
     2341 ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac
     2401 taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     2461 cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtttatc cggatgcccg
     2521 cattgcgttg ctggaaaaag agtccggccc ggcctgtcac cagacgggcc acaacagcgg
     2581 cgtgatccat gccggggtct attacacgcc cggcagcctg aaggcgcagt tttgcctggc
     2641 gggaaaccgc gccaccaaag ccttttgcga tcaaaacgac attcgctatg acaactgcgg
     2701 caagatgctg gtcgccacgt ccgatctcga aatggaacgg atgcgcgcgt tgtgggaacg
     2761 cacggcggcg aacggtatcg agcgcgagtg gttaaacgcc gaggaactgc gcgagcgcga
     2821 accgaatatc accgggctcg gcggcatttt tgtgccgtcc agcggcattg tcagctatcg
     2881 cgaagtcaca gcggcaatgg caaaaatctt ccaggccaga ggcggtgaga ttatttataa
     2941 cgccgaagtc agcgccctca gtgagcataa aaacggcgtg gtcgtccgaa cccgtcaggg
     3001 cggcgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
     3061 gaaaatgctc ggcctcgaac cgggttttat tatctgcccg ttccgtggcg agtatttccg
     3121 ccttgcgccg gagcataacc agattgttaa ccatctgatt taccccattc ccgacccggc
     3181 aatgccattt ttgggcgtgc atctcacccg aatgatcgat ggcagcgtca ccgtcgggcc
     3241 aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct catttagcga
     3301 cacgctggag attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     3361 aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcgcc tggtgcaaaa
     3421 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca
     3481 ggcagtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccacgcac
     3541 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     3601 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     3661 agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
     3721 acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg
     3781 aatggctgga cgccaacaat ggtgaagtca tcgacgtcac caatccggcg aacggcgaca
     3841 agctgggtag cgtgccgaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
     3901 accgcgccct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
     3961 actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     4021 agggcaaacc actggccgaa gcgaaaggtg aaatcagcta cgccgcctct tttattgagt
     4081 ggtttgccga agaaggcaaa cgcatttatg gcgacaccat tcccggtcat caggccgata
     4141 aacgcctgat tgttatcaag cagccgattg gcgttaccgc agctatcacg ccgtggaact
     4201 tcccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggc tgcaccatgg
     4261 tgctgaagcc cgccagtcag acaccgttct ctgcgctggc gctggcggag ctggcgattc
     4321 gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcaggc gcggtcggta
     4381 atgaactgac cagcaacccg atggtacgca aactgtcgtt taccggttcg accgaaattg
     4441 gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     4501 gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc
     4561 tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc
     4621 aggacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcaca
     4681 tcggcgacgg gctggataaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     4741 caaaagtgga agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg gtttgcggcg
     4801 gtaaagcgga tgaacgcggc ggcaacttct tccagccgac cattctggtg gacgtgccgg
     4861 ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct cgccccgctg ttccgcttta
     4921 aagatgaagc cgatgtgatt gcgcaagcca atgacaccga gtttggcctt gccgcctatt
     4981 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcgctggag tacggcatcg
     5041 tcggcatcaa taccggcatt atttccaatg aagtggcccc gttcggcggc atcaaagcct
     5101 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     5161 tgtgcatcgg tctttaactg gagaatgcga atgaacagca ataaagagtt aatgcagcgc
     5221 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     5281 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     5341 gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg
     5401 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     5461 gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct ggttacgacc
     5521 ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa acgtagcggc
     5581 accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     5641 aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt
     5701 tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc
     5761 ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     5821 gaaggcggtt tctacgccgc gtcgccagcc tttatgcagc gtttacgcgc gctgtgtgac
     5881 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     5941 ctgtttgcga tggagcagat gggcgttgcg ccggatctca ctacctttgc gaaatcgatc
     6001 gcgggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga tgccgtcgct
     6061 ccaggcggtc tgggcggcac ctatgccggt aacccgattg cctgcgtggc ggcgctggaa
     6121 gtgttgaagg tgtttgagca ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag
     6181 ctaaaagacg gattgttggc gatagccgaa aaacacccgg agatcggcga cgtacgcggg
     6241 ctgggagcga tgattgccat cgagctgttt gaagatggcg atcacagcaa accggacgca
     6301 aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gcctgattct tctctcctgc
     6361 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     6421 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagcagta gctatgtccg
     6481 tttgtaagag ttaaacgttg ctaagggaat gataacgatg cagtaaaaaa taataacgat
     6541 gaggcgatcg acaaagttaa aaacgactgc ggactgtccc ctcgctcctc tggggagagg
     6601 gttagggtga ggggtaatca cgcggctaca ccctcacccc ggccctctcc ctaagggaga
     6661 gggagaaaac tctcccgctt tcgtttttaa gattttgttg accgtctcaa ttaaacgcgt
     6721 gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga gaggattcag
     6781 gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac gccacgtcac
     6841 catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat
     6901 cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccggattac tggtggttat
     6961 gattatgcgg atgttggcgg aaatggcagt tgccacgccc gataccggtt cgttttccac
     7021 ctatgccgat aaagccattg gccgctgggc gggttatacc atcggctggc tgtactggtg
     7081 gttttgggta ctggttatcc cgctggaagc caacatcgcc gctatgatcc tgcactcgtg
     7141 ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct taactggcag
     7201 taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc tgtgcaaagt
     7261 catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt tttaccctta
     7321 tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc ccaacggctt
     7381 cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg cagaaattgt
     7441 caccattgcc gccgcggaat ccgacacgcc ggaaaaacat attgtccgcg ctaccaactc
     7501 ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtag tggcgttaat
     7561 accgtggaat atgccggggc tgaaagccgt tggttcttat cgctcggttc tggaattgct
     7621 caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg taaccagctg
     7681 tctgaactcg gcgctgtata ccgcgtcaag gatgctctac tccttaagcc gtcgcggtga
     7741 tgcgcccgcg ctaatgggca aaatcaaccg cagtaaaacc ccgtatgtgg cggtgttact
     7801 ctccaccgga gcggcatttt taacggtggt ggtgaactat tacgcacctg cgaaagtgtt
     7861 taaattcctg atagacagct ccggcgctat cgccctgctg gtttatttag tcatcgccgt
     7921 ttcacagttg cggatgcgca aaattctgcg agcagaagga agcgaaattc gcttgcgcat
     7981 gtggctttat ccctggctca cctggctggt catcggcttt attacctttg tgttggtagt
     8041 gatgctattc cgtccggcgc aacagttaga agtgatctcc accggcttat tagcgatagg
     8101 gattatctgt accgtgccaa ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac
     8161 acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat tacgtctctg
     8221 gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa
     8281 ttacggatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt gcgggaagca
     8341 ctttctcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg
     8401 gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa tatggaagcg
     8461 atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca
     8521 aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca gcgagaaaat gcttgatgag
     8581 tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg ttctcactat
     8641 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt tatctggctg
     8701 cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca ccagaccctg
     8761 actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg ccagcattta
     8821 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttccggaat
     8881 acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt tattcgcttc
     8941 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
     9001 gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg ttttcacctg
     9061 atcatcgaca ctggcaatac cggaaatatt cccaaccgca acaaggattt tctccttcgc
     9121 ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt gaatgttcac
     9181 tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct tcgcctgatc
     9241 gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat ctttcacaaa
     9301 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
     9361 gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt ttgcacccag
     9421 aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt aataatgaac
     9481 gcccaaccga accctttacc gagcagcacg ccgagcggcg gcagaatgat ggtgatgacg
     9541 attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt aaaaagagat
     9601 tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta cgcttttact
     9661 ctcatttacg ctgctgaggc tggtatatgt tgcattttta gtttagagtt tacttaattt
     9721 agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg ccgaacaggc
     9781 agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt gcatgcttag
     9841 cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga gtgcctctgc
     9901 gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc aacgggatgc
     9961 ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg ccaccctgaa
    10021 aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc cgcatgatgc
    10081 acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgacgctg atgaatatct
    10141 tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac agtcaggtct
    10201 tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta aacgcaccag
    10261 taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt tactcgaaga
    10321 tgggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat ctcaaccctt
    10381 gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg gcgttgtact
    10441 gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg ccgggttact
    10501 gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga tgccgtggaa
    10561 ccaacgaact tgagaagcga cgccggacgc gccctagcag cgacatccgg cctcagtaat
    10621 tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat tggcttcggt
    10681 gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcggtgaa tttatatttc
    10741 gccggacgcg gctggcgttt tttaccagcg cgtggtgcag cagcagagct attacctaat
    10801 aactcttccg ggttaattcc gtcagccttc atcagctcca gccaggtgct aattttttcc
    10861 tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc tttagtgaca
    10921 accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg agccatcgca
    10981 cggagagtgc gaatgttatt taaactttgt aacattacgg acataataaa gaaaaccttt
    11041 taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta ttatatttcc
    11101 acaaaattta atctatttac agtcctgcgc gagattattt cagctgttat tttctgaaac
    11161 tctcagaata atttcaagat tatatattag ccattttgat gagtaattta gattattggt
    11221 gaacagagtg gtgctctctg ctcgctaatt ccgcaagcct gacaaa
//
LOCUS       DAZKGS010000138        11119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKGS010000138 DAZKGS010000000
VERSION     DAZKGS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..583
                     /locus_tag="ACHOQY_003718"
     CDS             98..583
                     /locus_tag="ACHOQY_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9679739.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            599..1075
                     /gene="radC"
                     /locus_tag="ACHOQY_003719"
     CDS             599..1075
                     /gene="radC"
                     /locus_tag="ACHOQY_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9679740.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            1144..1365
                     /gene="yeeT"
                     /locus_tag="ACHOQY_003720"
     CDS             1144..1365
                     /gene="yeeT"
                     /locus_tag="ACHOQY_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9679741.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1528..1896
                     /locus_tag="ACHOQY_003721"
     CDS             1528..1896
                     /locus_tag="ACHOQY_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9679742.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            1986..2363
                     /locus_tag="ACHOQY_003722"
     CDS             1986..2363
                     /locus_tag="ACHOQY_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9679743.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            2360..2509
                     /locus_tag="ACHOQY_003723"
                     /pseudo
     CDS             2360..2509
                     /locus_tag="ACHOQY_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            2609..2689
                     /locus_tag="ACHOQY_003724"
                     /pseudo
     CDS             2609..2689
                     /locus_tag="ACHOQY_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3505..3876
                     /locus_tag="ACHOQY_003725"
     CDS             3505..3876
                     /locus_tag="ACHOQY_003725"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9679744.1"
                     /translation="MLFLPPLQLSAPGSIGFKNRRQLAAWLGLVPRQCFSVGKNTLGS
                     ISKRDDVYIRTLLFHGAKTVSNARKGRQVQESWLGRPVSRRNKNVATVALNNKNARVL
                     WVLLTNDKEISPEEVMVAAYM"
     gene            4147..4512
                     /locus_tag="ACHOQY_003726"
     CDS             4147..4512
                     /locus_tag="ACHOQY_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP9679745.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(4613..4942)
                     /gene="tmaR"
                     /locus_tag="ACHOQY_003727"
     CDS             complement(4613..4942)
                     /gene="tmaR"
                     /locus_tag="ACHOQY_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9679746.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5114..6172)
                     /gene="yeeA"
                     /locus_tag="ACHOQY_003728"
     CDS             complement(5114..6172)
                     /gene="yeeA"
                     /locus_tag="ACHOQY_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9679747.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRHLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(6370..6843)
                     /gene="sbmC"
                     /locus_tag="ACHOQY_003729"
     CDS             complement(6370..6843)
                     /gene="sbmC"
                     /locus_tag="ACHOQY_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9679748.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6961..8127)
                     /gene="dacD"
                     /locus_tag="ACHOQY_003730"
     CDS             complement(6961..8127)
                     /gene="dacD"
                     /locus_tag="ACHOQY_003730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9679749.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            8336..9763
                     /gene="sbcB"
                     /locus_tag="ACHOQY_003731"
     CDS             8336..9763
                     /gene="sbcB"
                     /locus_tag="ACHOQY_003731"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9679750.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9806..10033)
                     /gene="tsuB"
                     /locus_tag="ACHOQY_003732"
     CDS             complement(9806..10033)
                     /gene="tsuB"
                     /locus_tag="ACHOQY_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP9679751.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10047..11105)
                     /gene="tsuA"
                     /locus_tag="ACHOQY_003733"
     CDS             complement(10047..11105)
                     /gene="tsuA"
                     /locus_tag="ACHOQY_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP9679752.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         2940 a         2885 c         2622 g         2672 t
ORIGIN      
        1 attgtgggtg atggcggaaa ccctgctgga gagcctgcgc tgattcagcc attaccctga
       61 aaaccacgca tttatttgtc ctttaaggag tcatttcatg aaaaccattt cacataattc
      121 caccacatct tctgtttccg taaccgctgc atcagggaat gaccagcctc agttggttgc
      181 caccatagtc cctgatgaac agcgcatcag cttctggccg cagcattttg gcctcattcc
      241 acagtgggtg accctggagc cccgtgtttt cggctggatg gaccgtctgt gcgaagacta
      301 ctgcggtggt atctggaatc tgtacactct gaacaacggc ggggcattta tggcccccga
      361 accggatgac gatgatgacg aaacatgggt actgttcaat gccatgaacg gcaaccgcgc
      421 tgaaatgagc ccggaagccg ctggcattgc tgcctgtctg atgacgtaca gtcatcatgc
      481 ctgtcgtacg gagtgttatg ccatgacggt ccattattac cggctgcggg attacgccct
      541 gcagcatccg gaatgcagcg ccattatgcg catcatcgac tgaatggagg cagggacaat
      601 gcagcagctt tcctttctgc ccggagaaat gacgcccggc gagcgcagtc tcattctgcg
      661 ggctctgaaa accctggacc gccatcttca tgaacccggt gtggccttca cctccacccg
      721 tgcggcacgg gaatggctga ttctgaacat ggcgggactg gagcgtgaag aattccgggt
      781 gctgtatctg aataaccaga atcagctgat tgccggtgaa accctcttca ccggcaccat
      841 caaccgcacg gaagtccatc cccgggaagt gattaaacgt gccctgtatc acaatgccgc
      901 agccgtggtg ctggcacaca atcacccgtc cggtgaagtc acccccagta aggcagaccg
      961 ccttatcacg gaacgtctgg tacaggcgct ggccctggtt gatatccggg tgccggacca
     1021 tctgatagtc ggtggcagcc aggttttctc ctttgcggaa cacggtctgc tttaacccgt
     1081 caccgtcaca accacatcac acctgttttc acttttatct tctgtcttca gaggtatcac
     1141 actatgaaaa tcatcacccg tggtgaagcc atgcgtattc accaacaaca tccgacatcc
     1201 cgtctttttc cgttctgtac cggcaaatac cgctggcacg gcagtgctga agcgtatacc
     1261 ggtcgtgaag tgcaggatat tcccggtgtg ctggccgtgt ttgcagaacg ccgtaaggac
     1321 agttttggtc cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg
     1381 aaaacgagta cgactggcag agctcagacg ccgctgtgga tgaggtcctg ctcatcaacg
     1441 aatacacctt taccgaagcc ggtctgcgcg ccggctgacc cgtcatcgtt aaactatcat
     1501 tcaccgattt tctgtgagga ttttatcgtg tcagacacac tttccgggac aacgcatccc
     1561 gatgacaatg acgaccatcc ctggtgggga ctaccctgca cagtgacacc ctgtttcggc
     1621 gcccgtctgg tgcaggaggg taaccggttg cattaccttg ccgaccgtgc cggtatcaga
     1681 ggccggttca gagatgcgga tgcgtaccct ctggaccagg cctttccgct gctgatgaaa
     1741 caactgaaat taatgctcac cagtggtgaa ctgaatcccc gccatcagca taccgtgaca
     1801 ctgtatgcga aggggctgac ctgtgaagcc gacaccctcg gctcctgtgg ttacgtttat
     1861 ctggctgttt atccgacacc tgaaacgaaa aagtaaacca ccggaataac cctctactcc
     1921 ggcctggtgt tttcaccacg ccactgtccc attttttatc tctgcatatc aggaaaatct
     1981 tcagtatgaa aacattaccc gacacgcatg tacgggaggt atcgtgctgc ccgtctctcg
     2041 tcaccatctg gcagacactg ctcacccgac tgctggacca gcactacggc cttacgctga
     2101 atgacacacc gttcgctgat gaacgtgtga ttgaacagca tattgaggca ggcatttcac
     2161 tgtgtgacgc ggtgaacttt ctcgttgaaa aatacgcgct ggtacgtacc gaccagtcgg
     2221 gattcagcgc ctgtacccac tctcagttaa taaacagcat cgatatcctc cgggctcgcc
     2281 gggcaacagg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggga
     2341 agcatccgga ggcgaaacga tgaaactggc cctgacgctg gaagccgaca gcgttaacgt
     2401 gcaggcgctg aacatggggc gcattgtcgt tgacgtcgat ggtgttaatc tcgctgaact
     2461 gattaacaag gtcgctgaaa acggtgattc actccgcgtg gtggatgaac gtgactctac
     2521 cgaaacaccg gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgataca
     2581 gggtatagtg ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga
     2641 agatgtcctt accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa
     2701 caggcttcat ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga
     2761 aaggaaaacc gataataatg gaatggacgc ccctaccatg aagcaacggt aggatattac
     2821 tgataaacct tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca
     2881 aaaaatgaat tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg
     2941 gattaaacgt tccctgatgc ccgtattctt cgcgaatatg tcttcctgtc ggattgggat
     3001 ggaggtctgc gccggagcgc gtcattagtg agaatgctgt ctacttaggg gcataatgtc
     3061 agactaatgc ccgcgatttg taaagccata tgtgaaaaca aacaagaatg atatggctgc
     3121 tgccgaggcc atctgtgaag ccgttacccg acctaatatg cggtttgtca gcatcaagac
     3181 gcctgagcag tagtcattac tgtcttttca tcgtgcccaa acgggatttg ttaaatcaag
     3241 ggctgcgcag ataaaccaga tcagagaatt actggctgaa tttggcatcg ttttcccgcc
     3301 cgacattgta gcattcagtc gtcatgtccc tgccctgcct gaagacgcgg aaaactgttt
     3361 aacaatgccg ttcagacagc tgtcgtccta tctctaatga aaatgtgaaa caactgagcg
     3421 tacacattga aaccatagaa gccgtgagca acgaacacgt cgggaacgac gaactgtgta
     3481 ggaaaattgc caccatttcg gggattgcta ttcttaccac cactgcaatt gtcagcaccg
     3541 ggtagtatcg gcttcaaaaa ccggcgtcag cttgctgcat ggcttggact ggttccccga
     3601 cagtgcttca gcgtaggtaa aaacacgctg gggtctatca gtaaacggga cgatgtctat
     3661 atcagaacgc tactgttcca tggtgcgaag acagtgtcaa acgccagaaa gggcagacag
     3721 gtccaggaaa gttggctggg cagaccagtc tcgcggcgga ataaaaatgt tgccaccgta
     3781 gcgctgaaca acaaaaatgc cagagtgctc tgggtattgc taaccaacga taaagagatt
     3841 tcaccagaag aagtgatggt agctgcatat atgtaaatga aactacttct gtctgttgaa
     3901 aaataaaacc aacctactga ttgctaagga taaacaagta ttaaaggcca gactgataag
     3961 accgcgacta agtgaacctg tatctgacat ggcatagctt cgctaatgtg tcatcatgat
     4021 taggactaaa tctgtgaatt cctttaggaa gcacggacac aggccaatct gagtccatat
     4081 gtatgattgg aatcttacac agatgacttt ttgacacggg gacttggcaa acaggggcga
     4141 ccatgtatgt cagatatgac aatctcactt ccgaaagaag tcaacgggca cgttatctgt
     4201 tcaaccagaa tccgacacat tctggctata cgaaagagca tacttatgca aatctatatg
     4261 cttttccggc aacaagcagg aatctcagcg ctgaggcagg aattagaggt aaaaacgccc
     4321 taccgggcca tgaatcaccc ggtcattgga gtagtaacca aagcagattt agccagcatg
     4381 gaacacatat ctttggtgaa atgttggtta cgggaagctg gagcgcacaa cgtgttagta
     4441 accagtgcgg ttaataacaa tggagttaca gaactttttg ccttgctgca tacagaagaa
     4501 ggctgttgct aatttcgata ggttctcaga tgagaatgat gacggtgata cacagataag
     4561 aatcaatgag caatgagggt tgccgggcaa ccctcattga ataaaacagg aattacttcg
     4621 cttcgccgtt tttcatttcg cccatagctt tcagcttttt ggagatatcg cggcgttctt
     4681 tggagagctc ggcatttttg atgatgtaat cgtcaacgcg atcttcatag tcacctttca
     4741 tgctggcgat gatgccctgg attgcttcaa cgctcatccc gggcttgatg taatcgctca
     4801 ggttgtccag caacaggacg cgcttctggt tgtcacggat ctttttctca acgtcctgaa
     4861 tttcacgttg cagtttgttc ttacgacgga acagacgaac aaattccagt acgtcctgga
     4921 atgaaggctt ggtagtttcc atttttatac ccctgataat gtgagagtcg gattcgttta
     4981 agcaaccgct attcgttagg gccaacatta ctcatagctg ccgcagatga caatgctttt
     5041 atcccttttc actatcatac cctttatcct tgctgaatcg aagcagcagc aagatgattc
     5101 tgaagttcag gaattatttg cgcaaggccc ggcaaatcag actcgatagc aactcaagct
     5161 gatgtgccgt ttccatgttc agccacacat aaccatagat tggtgtttcc acaaccttca
     5221 ggtcatggtg gttattcagc aactgacgca gctcttccac agcatcgatc aatttttccg
     5281 tattggcaaa aaccggctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta
     5341 tttgctgcat catgtgctgg gtatcacgaa gtttttgcgc gttcaataac acgaaatggc
     5401 tggggcgcgt ggcccagtat gcattgattt gcaactccag catacaaacc agatggcggt
     5461 taatggtctg gataccttca tatatcgatt ttggaatacg agtttctttg ctggcgggcg
     5521 caatcagccc acgcattttc acggcatcgg tcaggagttt ttgtagatgg ctttccagac
     5581 gtgggcgttc gagtaagttc ggtgagaatg cagattgata aacccgatta tactcggtca
     5641 gacttttcgc cagttgaatg cgccagtgga tgaacgcccg ttgtggccag ataccggtaa
     5701 acaacattgc cagtaaagag ccgaggatca catcgccgct tcgccataac gccgtatcaa
     5761 tttcacctgt cggggaaccc acaacaattg ccagcgtcac cccaatcaat aatccttgat
     5821 acggtttctt gcccagcgcc agccaaccgc aaagaaacat agccgccgcg caccagacta
     5881 acatcagcgg taacgaaatt aactccagtt gcagagcgat aagacctaaa atcgaaccca
     5941 acaccgtacc gccaatacgt tcgaaggcgc gagggacaac gttaccccag aacgagattg
     6001 gccccataat caccaccatg gtgaccagcg gccaggtgct ttccgggata gtaaacaggc
     6061 ggataatgag aaaagtgagc aggaatgcca gcgcgacccg agtaccatgc acaatgcggt
     6121 aatggcggta tacccggatt tcaaacgggc ttaatgactt atcggcacgc acgcagaaac
     6181 tccgtaatga agattaaaag tgctcataac tcatctgtcg gccagaaggc ccgcctctga
     6241 attatccgac ggacaaaccg ggaacagtgt aatttcccgg caggagcgaa aaaattgaaa
     6301 ggcgcatctt actcttttcg ctttcaaaaa aagagtggtc atcgcgttaa cacaccgccc
     6361 tgagatgaat tagtgatgtt ttggctgcac cgcaacatac atttcgatat cccagtaccc
     6421 atcttccgcg ccattgttca aataaacctc gaagcagggc tttggtaaca tttcataagc
     6481 actgtcctgc aagaggctat taaagaactg ataccagggt ttagcaaaat catcaccgac
     6541 tacacgagcc accgcaaccg catactgacc acctgaaatt tctgtcagaa tgacgccctc
     6601 actgttttcg gggagcgtaa agttattcgg caccgtcacg acgatgtcgc agcgtaattt
     6661 ttcggcgggt gtttcatctg gattgtcgta aaagacagca acccactcct tcggcacaat
     6721 atttttgcta tctacccaca tcatcaactg ctcaaagcct ttctttaccg tctgttccca
     6781 cgggccaacg agatggaaac ctgcaacggt acgtttatct tcctgcttaa tctcgtagtt
     6841 catgacgtcc cattaacact gtttttatat acagtataat tgcgatatta aaaccacaag
     6901 gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc aaaagaaagg
     6961 tcaggcctta tggtggaaat aatcactcag gcgagaaaac atgctgcctt ccccgacaga
     7021 ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa gttcaatttc
     7081 ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa gggtatattt
     7141 ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt gttccgttcc
     7201 caggtcgata ttttctttat cgccatacca gatgcgttcc gtaccgacct ttttcccacg
     7261 gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt ttcttgcctc
     7321 ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac gacgctgccc
     7381 atcgacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg ttttcaggcc
     7441 gtcaacattc atggttttat cccacaataa cccgttacgg ttttgctggg tgataccgtt
     7501 ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgtcgt ggatgatagc
     7561 gcgagaaagc acagctaaat cataagccga gctatgctga cctggtgcat ccagaccatg
     7621 cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt tcatcatttc
     7681 aacaaactgc cgttgcccac cggcaatata gtcagccagt gcaacacaag cgtcatttcc
     7741 ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc cctctttcaa
     7801 aaacatcagt gaagaaccga caaacactgg attatctttc gcccacgcat cgcgccccac
     7861 ggtgacaata tcgtctggcg taatgcgatg actatcgata gtgcgatcca cgacatagcc
     7921 cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac ccgcggtgag
     7981 gatctgaccg gtggtgtaat ccatcagtac ccaggagccg gcatgaatct ctggaggctg
     8041 aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa tgaacaaaga
     8101 agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa atagctgttc
     8161 tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt gactaccctc
     8221 tcatttttat ctgacatgat ctgttgtcac tcgctgtcaa attgtcgcgc taaagctgat
     8281 tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta acctaatgat
     8341 gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct ttggcacgca
     8401 ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgatgacg aattcaatgt
     8461 catcggcgaa cccgaagtct tttactgcaa gcccgcggat gactatttac cccagcctgg
     8521 agcagtatta attaccggta ttaccccgca ggagacacga gcgaaaggag aaaacgaagc
     8581 cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta ttctgggcta
     8641 caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta atttctacga
     8701 tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttactgg atgttatgcg
     8761 tgcctgttat gccctgcgcc cggaaggaat aaattggcct gaaaatgatg acggtctacc
     8821 gagctttcgc cttgagcatt taaccaaagc gaatggtatt gaacatagca acgcccacga
     8881 tgcgatggct gatgtgtacg ccactattgc gatggcaaaa ctggtaaaaa cgcgtcagcc
     8941 acgcctgttt gattatctct ttacccatcg taataaacac aaactaatgg cgttgattga
     9001 tgttcctcag atgaaacccc tggttcacgt ttccggaatg tttggtgcat ggcgcggcaa
     9061 tacaagctgg gtggcaccgc tggcgtggca tcctgaaaat cgcaatgccg taattatggt
     9121 ggatttggca ggagacattt cgccattact ggagctggat agcgacacat tgcgcgagcg
     9181 tttatatacc gcaaaagccg atcttggcga taacgccgcc gttccggtta agctggtaca
     9241 tatcaataaa tgtccggtac tggcccaggc gaatacgcta cgcccggaag atgccgaccg
     9301 actgggaatt aatcgtcagc attgcctcga taacctaaaa attctgcgtg aaaatccgca
     9361 agtgcgtgaa aaagtggtag cgatattcgt ggaagccgaa ccgtttacac cttcagataa
     9421 tgtggatgca cagctttata acggcttttt cagtgacgcc gatcgtgcag caatgaaaat
     9481 tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg ttgataaacg
     9541 aattgaaaag ctgttgttca attatcgggc gcgcaacttc ccggggacgc tggattatgc
     9601 cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgccagagt ttttacaagg
     9661 ctatgcagaa gagattcaga tgctggcgca gcagtatgcc gacgataaag aaaaagtggc
     9721 gctgttaaaa gcgctttggc agtacgcgga agagattgtc taatgtgaag cctccgccgc
     9781 tggtacggcg gaggacaatt ttgagtcagg ctttttgaac ggtgatgctc cacgctgcat
     9841 cgccaatctg ctgataatcg gtgatggcat gtccttcttc tgccgcccac tgtgggattg
     9901 cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg gccatttctg
     9961 ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc acatccagct
    10021 ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag tcgtgcctta
    10081 cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat cataaataca
    10141 aggccaatcc agccctgcca ggtcatcatt gcagtcatga ccagtccatt accgatagag
    10201 caaccacctg caatgctggc accgaagccc atcagtacac cacctaaccc gctacgtaat
    10261 gttgtttgtg catcagcagc gcgaacgcgg aactcacggc tcgctttggc tgcaataaat
    10321 gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt cacgtcaccc
    10381 gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc aaacatgcgt
    10441 ccggttgctt cactcagggg ccaagctaaa agcgcaatca aaccgatgag tacagcagtg
    10501 acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccggttcg gcgcggcggt
    10561 aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca gagcgttatc
    10621 accagcaaaa cggcaaccaa cggccacaca gacaaattaa aagtatcagc aatagagtta
    10681 tgttcagtag tgtagtgctg caaggtttga tttaaaccac tggcgtgtgg agaacgcatc
    10741 accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa tccctcaccc
    10801 gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag cccgaaaaga
    10861 tacccaccta taacagtacc aagccacggg aacgccccgg cttcgtaagt cagtaggccc
    10921 gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag caaggcgtaa
    10981 aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag gcaaaaacgc
    11041 ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa tatcattgaa
    11101 aacataaatt aattaacca
//
LOCUS       DAZKGS010000139        11112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKGS010000139 DAZKGS010000000
VERSION     DAZKGS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            140..769
                     /gene="yqiJ"
                     /locus_tag="ACHOQY_003734"
     CDS             140..769
                     /gene="yqiJ"
                     /locus_tag="ACHOQY_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9679753.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            796..2457
                     /gene="yqiK"
                     /locus_tag="ACHOQY_003735"
                     /pseudo
     CDS             796..2457
                     /gene="yqiK"
                     /locus_tag="ACHOQY_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            2696..2755
                     /locus_tag="ACHOQY_003736"
     CDS             2696..2755
                     /locus_tag="ACHOQY_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9679754.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            3072..3131
                     /gene="ibsE"
                     /locus_tag="ACHOQY_003737"
     CDS             3072..3131
                     /gene="ibsE"
                     /locus_tag="ACHOQY_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP9679755.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3251..4684)
                     /gene="hldE"
                     /locus_tag="ACHOQY_003738"
     CDS             complement(3251..4684)
                     /gene="hldE"
                     /locus_tag="ACHOQY_003738"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9679756.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNITSLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4732..7572)
                     /gene="glnE"
                     /locus_tag="ACHOQY_003739"
     CDS             complement(4732..7572)
                     /gene="glnE"
                     /locus_tag="ACHOQY_003739"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9679757.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFSALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(7595..8896)
                     /gene="ygiF"
                     /locus_tag="ACHOQY_003740"
     CDS             complement(7595..8896)
                     /gene="ygiF"
                     /locus_tag="ACHOQY_003740"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9679758.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9138..9758
                     /gene="ygiM"
                     /locus_tag="ACHOQY_003741"
     CDS             9138..9758
                     /gene="ygiM"
                     /locus_tag="ACHOQY_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9679759.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            9822..11060
                     /gene="cca"
                     /locus_tag="ACHOQY_003742"
     CDS             9822..11060
                     /gene="cca"
                     /locus_tag="ACHOQY_003742"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9679760.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         2678 a         3043 c         2816 g         2575 t
ORIGIN      
        1 tgaaaaataa aaaaccatat taaaaaactt tctcttccag aacttctcta acatgtgtta
       61 ttattaacaa agataaatgt aagcaaggaa actatcccaa agtaaatatt gcgtcactaa
      121 atggacattg gagtgacata tgattttatt cgccgactat aatacccctt atcttttcgc
      181 tatttccttt gtactgttga tcggcctttt agaaatttta gcgcttatct gcggtcacat
      241 gctctccggt gctcttgatg cacatcttga ccattatgat tccataacca caggtcatat
      301 tagccaggca cttcattatc tcaatatcgg aagattgcca gctctcgtcg ttctctgctt
      361 gctggctggt ttttttggtc ttattggcat cctgttgcag catgcctgca tcatggtctg
      421 gcaatcacca ctgtcgaacc tgttcgtggt tcctgtcagt ttactgttta cgattattgc
      481 ggtgcattat accggtaaaa tagttgcgcc ctggattcca cgcgaccata gttcagctat
      541 tacagaagaa gaatatattg gtagcatggc attaattacc ggtcatcaag ccacttcagg
      601 taatccctgc gaaggaaaac tcaccgatca atttggccaa atacattatt tactactgga
      661 gcctgaagaa ggaaaatttt tcactaaagg agacaaagtg ttaattattt gccgactttc
      721 tgcaacgcga tatttggcgg aaaacaatcc ctggccacaa atattgtaat ttattaatca
      781 aaaggaaatt taataatgga tgatattgtt aattctgtgc cctcctggat gtttaccgcg
      841 attattgccg tatgcattct gtttattatt ggaattattt tcgccaggct ctatcgtcgc
      901 gcttcggcag agcaagcttt tgttcgtact ggtttaggtg ggcaaaaagt ggtgatgagc
      961 ggtggcgcaa tcgtgatgcc gatctttcat gaaataatcc ccatcaatat gaatactctg
     1021 aagctggaag tcagccgctc aaccattgat agcctgatta cgaaagatcg tatgcgtgtc
     1081 gatgtagtag tcgctttctt tgtacgggta aaaccgtctg tagaaggcat cgccactgcg
     1141 gcgcagacgt tggggcaacg cacgctatca ccagaagact tacgtatgtt ggttgaagat
     1201 aaatttgtcg atgccctccg tgcaacagct gcacaaatga ccatgcatga gttacaggat
     1261 acccgcgaga acttcgtgca gggagtacaa aatactgtcg cagaagacct gtcgaaaaac
     1321 ggtcttgagc tggaaagcgt ttcacttacc aactttaacc agacctcgaa agaacatttc
     1381 aatccgaaca atgcctttga cgccgaaggt ttaaccaaac tgactcagga gacggagcgc
     1441 cgtcgtcgcg aacgtaacga agttgaacag gatgtagaag ttgcggtgcg tgagaaaaac
     1501 cgtgatgcac tttcgcgcaa gttggagatt gaacaacaag aagcgtttat gacgcttgag
     1561 caggagcagc aggttaaaac ccgtactgcc gaacagaatg cacgtattgc ggcttttgaa
     1621 gctgaacgtc gtcgtgaagc agagtagacg cgaattctgg ctgaacgaca aattcaggaa
     1681 acggagatcg agcgcgagca ggccgtccgc tcaagaaagg ttgaggctga acgtgaagtt
     1741 cgtattaaag agatcgaaca acagcaggtc accgaaatcg ccaaccagac gaaatcgatc
     1801 gctattgccg ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa ccttgcactc
     1861 gcagaagcag taagtgcgca acaaaacgta gaaaccactc gccagaccgc agaagccgat
     1921 cgtgctaaac aagttgccct aatcgctgcc gcgcaggatg cagaaaccaa agcggttgaa
     1981 ctgaccgtgc gggcgaaagc agaaaaagaa gccgcagaga tgcaggcggc ggctatcgtt
     2041 gagttagccg aagctacacg taaaaagggt ctggcggaag cagaagcaca acgtgcgctg
     2101 aacgatgcta tcaacgtact ttctgatgaa caaaccagcc ttaaattcaa actggccttg
     2161 ttgcaggcgc tacctgcggt aatagaaaaa tccgttgagc cgatgaaatc tattgacggt
     2221 atcaagatta ttcaggtcga tggtctgaat cgtggcagcg ctgcgggtga tgcaaacacg
     2281 ggtaatgtgg ggggcggaaa cctggcggaa caagcattat cagccgctct ctcttaccgc
     2341 acacaggcac cgctgattga ctccttgctc aatgaaattg gcgtttcagg cggctcactg
     2401 gcggcattga cttcaccctt aacctcaaca actcccgtcg ccgaaaacgt agaataaatt
     2461 ttcagctatg aacgtccctg tcacaaaaag ccggggattt tttatatctg cgttccgcta
     2521 aaaactgcaa atgatcaggt cgttgcaacg ttttgcgtga ccgctcgggg aaggcaaaac
     2581 tgcctctggg aaagcgttgc gcgaggtccg gcgctcatca acaatcgggg agtcagcaaa
     2641 gggctgaaac gggaaagccc ctccctagga aggggcctta cagaaggaaa gggttatgat
     2701 gaagctcgtc atcatactga ttgtgttgtt aatcgtaagt ttcgcagctt attaacagcc
     2761 aatcagaggg gggagaaatc ctccctcacc cttgttcctt tactttaggt tgaaaaaaaa
     2821 tcagcgtcaa gaaggtttca ttaacacata tccctggccc aaaaagccgg ggatttttta
     2881 tatctgcgtt ccgctaaaag gtgcaaatgc tcaggacgtt gcagcgtttt gcgtgaccgc
     2941 tcggggaagg caaaactgct tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa
     3001 tcggggagtc agcaaggggc tgaaacggga aagcccctcc cgaggaaggg gccttataga
     3061 aggaaagggt tatgatgaag ttcgtcatca tactggttgt gttgttactg ttaagtttcc
     3121 cgacttacta acaactgatc agagggggga gaaatcctcc cttacccttg ttcctttact
     3181 ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct acgaagcgag atctgtgaac
     3241 cgctttccgg ttagcctttt ttatcctgtt ggatcttctt gatgatgtta gtggtcgagc
     3301 aaccgtcttc gaagttgagc accagcactt cgccgccgtt ggcccagact tctttactcc
     3361 cggcgatctc ttctggttta tagtcgccgc ccttcaccag taaatctggc aggatcccgg
     3421 cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac ccagtcgacc gcttccagtg
     3481 cgcccagcac aatcatacgc tgttcgagcg ggttaacggg gcgggaatcc cctttcagcc
     3541 gtttggtgga ggcatcgctg ttcacagcga caatcagacg gtcacccagt ttgcgagcat
     3601 ttgccagata ggagacgtgc ccggcgtgca ggatgtcaaa gacaccgttg gtcattacca
     3661 ccttttcgcc gcgtttacgc gctgccgcta cagcaagctt cagttcctct tcggtcatca
     3721 caccaaagcc ggtatctgcg cgcccacgca ctgcattttc cagctcgatc ggcgaaaccg
     3781 tggaggttcc cagtttgccg accaccacgc cagccgccgc attggcaaag aagcaggctt
     3841 cttccagcga attacccgct gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg
     3901 caccggtaac gtcatacact tcctgcgcct gggttggcat atgcagcggc gctttacccg
     3961 gttgcagcag cgacataccc tgttcggaac gggtcactaa cagagccgag agttcgtaat
     4021 cggcaatcag tttcatgccg cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa
     4081 cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc gccgcggtag cgctcaaaat
     4141 cggtaccttt tggatcaatc agcaccggaa cgcccgcttt acgcgccagt tggatcatct
     4201 gctgtacgct tgccagcgca cctttggcgt agtcagaaag caccagcgcg ccaatcgaac
     4261 tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc aacaccttcg aaaccttctt
     4321 caaaatccag gcggatcagc tgttggttgc gggaaagtac ccgtaatttg gtgatagtcg
     4381 gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac gtcggccaga gatttactca
     4441 gcgcacgcgc cgcatcgtca atgcccgtca gcccgaccag gcgagcattg gcaccgagag
     4501 aagtgatgtt cattgccacg ttagccgcgc cgcccggacg ttcttcgatg gtattgactt
     4561 taaccacggg caccggcgct tccggcgaga tacggctggt gggaccgtac cagtaacgat
     4621 ccagcatcac atcaccaacc accatcactc ctgcacgttc aaactctggc agcgttactt
     4681 tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat aataccatac ttcattcttc
     4741 caccagccac ttctgccagc ttgcccgcac cagttcacgc tctgcggtga agcaatcctc
     4801 cggcacatgg cccggcaatt cctgtaatgc cagatgatga agttcatcgc gcagcgtagt
     4861 atacgcacgg gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt gcgccagtag
     4921 ttccagaatg cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg
     4981 caacaccaga tattgggtaa taaattcgat atcggtaatt ccgccttcat cagctttgat
     5041 atcaaagcga tcgcgatgtt tattgccgag atgagcgcgc attttctcgc gcatttcccg
     5101 cacttccgtt tgcagagttt taccttcacg cggcagcgtc ataatctcgc gacgcaccgc
     5161 gtcaaagtgc gcggtgagct gcggatcgcc gtacactacc cgcgcacgca ccagcgcctg
     5221 atgttcccac gtccaggcct cgtttttctg ataatcggca aatgcttctg cggatgtcac
     5281 cagcattccc gcggccccgg acgggcgtag ccgtgcatcc acttcataca aaatgccgga
     5341 agaggtacgc gtactgaaca gatgcataat gcgttgcgcc agacgcagat aaaactgtcg
     5401 cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt gggcaatcat ggaggaagat
     5461 caggtcaaga tcggaactgt agcctaactc ccagccgccc agcttgccgt agccgaccac
     5521 cgcaaaacca cggccttcgc gatcgttgag gtgatttggc ttaccgtagc gggccaccat
     5581 ttgaacccac gcctgctgaa cgacggcatc tatcatagcc tctgccagcc aggttaagtg
     5641 atcgctcact ttcatcaccg gtagcgtacc agcgatatcc gctgcggcga tgcgtaacag
     5701 ctgtgcctgt ttgaactgac gcagcgcctc aagctgttgc tcttcgtcat cttccggcac
     5761 gcgcagcaaa tactggcgca actcatcgcg gtaggcatcg gtcgccgtcg gctggtaaag
     5821 ggtgtttgga tcgagcaatt catccagcaa taatggataa cgcgccagtt ggctggcaat
     5881 catcggcgac gcggcacaca gagaaatcaa atgtttaagc gccgcgggga attcactgag
     5941 caattctaaa taggtggtgc gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag
     6001 cgtaacggca gcgtcttcac gcgcacagac atcacttagc agatgcggca tcagatggtc
     6061 gagcacctga cgtcctcgcg gcccgatggt gcgcttatcc agctctttgc ggaaatcggc
     6121 aatcagcgtt agcacctgtt tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt
     6181 gtcatcttcc tgcaacgcat cctgccacag ctcacgccac tgttccgaca gcgactcttc
     6241 ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca cattggtcat
     6301 atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca aagtccatcg cccacgccag
     6361 ccgcgcacgg ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg
     6421 cagcaggttt tccagacgcc gcaggaacag atacgccact cgcaattgtt cagcatcgtt
     6481 ttcagaaagc agatgcagcg cggcaatgac gctgagcgtt ggcagtaaag agcgcgactg
     6541 cagcgacggt tcgcgcccac cgcgaatgag ctggaacacc tgaacgataa attcaatttc
     6601 gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa ccacgtcgac gcacttcacg
     6661 ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga tgtaacgacg
     6721 gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca tagacgcctt cgctatcgcc
     6781 cataatccgc gccttgacca tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata
     6841 atcctccaac gcggaaaagc tcagcaccag cgggccactt tcgccaaacg gacgcagccg
     6901 catatccacg cgatagacga agccatcctg cgttggttga tccaacactt taatcagccg
     6961 ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc cggcgtccgc cctgcgtaca
     7021 accatgttcc ggccaggcaa aaatcagatc gatatctgag gagaaattca gttccccacc
     7081 gcccagctta cccatgccta aaatcagcag cggttgcgct tcgccctgcg cattgcacgg
     7141 cgttccccac tcgcggcagc aagcgtcata cagccagtca cgcgcggcga caatcagcgt
     7201 ctccgccaga tggctgagct gctgcaatat gctctcttca gtaaccaaag ccagcgtttg
     7261 cgcccaggcg atgcgcacca taatgcgccg ccggaatagc cgcagctcgc gcattaaccc
     7321 ggcttcgtca ctcacattac tgagcgcctc ctgcaaccat gccgcgtaat gctgccattc
     7381 gtcggcctgc ggcggttggc tttccagttc cgtcagccac tctggatgca caatcacgct
     7441 gtcctgcaca aaatcactaa aagtaagtac tgacttcgcc tgtgcgctaa gtgattcctc
     7501 ggctaaaggc tctggcagcc gctcaacaac ggtctgccag tactgctgta acggtgaaga
     7561 gagcggcttc ataagcgatt ttatccttgc ctgattaacg ttttccgctg tgcaaccaga
     7621 acggttcctg attgtttgcc tcattacgga aatgttcaat ttcaatgcgt tgcccggtcg
     7681 caatagcgtg acgcagcccc tgccagttct ccagccaggc ttgcgcgacg acaggatcat
     7741 agtaacctgc cagcagcagt attgagtcaa tatcacgcgt caggcgtggc aactgatcat
     7801 ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa
     7861 gatggatatc ggcaaagcgt ttgaaggaat cgctgatttt accctgcgct ttggcatcta
     7921 aaaacggctg ccaggctttg cttaccaacc attcggtcaa cgccagcttt gccattgccg
     7981 tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga agcgatggtc gcttcgcatt
     8041 gagtcagcaa atcacgtaag tgagtgctcg ctttacgcgg cacaatgcca ccgaacagca
     8101 tcagtgcatg gcgaacaagg ccaatggctg ccagcacctg ctctttcgcc gcatcgttgc
     8161 cgcgtaccca cagttcttca tgatactgcc attgcgctaa cgccagctcg aacgccgctt
     8221 ccagcccctg ttccacatcg gcttttgccg caacatgcaa aatggtggtc ggtttgattt
     8281 cacgcgccgg attgccctgc gccagatgat agccacgcgc cgctttgctc aggctgccct
     8341 ggcgtaatcc ggtttgcgat accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc
     8401 cgctaagcag ttccagttcc agctcacaga taggttcagc aaattccccc gctttcacct
     8461 ccccctgatc gagagcgatt tcaattcggc tgtcatcgac cgccaccagc catttttcgc
     8521 gataaaaatc ggtgctgaac agcggttgta cgcgggaagc gagatcggcg ggcaattcgc
     8581 cgttcggcca gacttccgtc ggtaactgcg ccaggtcgag cgtcggttcg ctcaacgcca
     8641 cgttatattc cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact ttcatggtca
     8701 tctcatagcg accgttttcg ccacgaatac gtaagcccat atcgtgccca cgcagccagt
     8761 tatccggcgt ttcgtagtaa atattcagca actgcacggg gtcatggtgc tcgccgccca
     8821 gcgtattgag atggtcacgc aacgcctcaa cggcactgtg attaacaata aactttaatt
     8881 cgatttcctg agccatggcc tttacttatc ggttatgtca cataagtgac gatgaacggc
     8941 gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg ccatgcaacg
     9001 ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag aggattccga
     9061 ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc actttccatg
     9121 acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta ctcgcactta
     9181 gcgccactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgatgaa ctgaatacct
     9241 gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac gccggcgagg
     9301 aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa gacagctctg
     9361 gccgtaccgc ctggatcccg ttgaaacaac ttagcactga gccaagcctg cgctcccgtg
     9421 tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat atcgataaca
     9481 cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca
     9541 acggattaaa agaagaaaat cagaaattga aaaacgagct gattgtcgcg cagaaaaagg
     9601 tcgatgccgc cagcgtacag ttggatgaca aacagcgcac catcatcatg caatggttta
     9661 tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg tctggtactg ccacacctga
     9721 tcccaacgcg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc gttgcactgt
     9781 cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt tatctggtcg
     9841 gtggtgctgt tcgggatgca ttgttagggc taccggtcaa agacagagat tgggtggtgg
     9901 tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc cgcgattttc
     9961 ctgtgtttct gcatccgcta acgcatgaag agtatgcgct ggcacgtacc gaacggaaat
    10021 ccggttccgg ttacaccggt tttacttgct atgccgcacc ggatgtcacg ctggaagatg
    10081 atcttaagcg tcgcgatctg accattaatg cgctggctca ggacgataac ggcgagatta
    10141 tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat gtttcccccg
    10201 cttttggcga agatccgtta cgcgttctgc gcgtggcgcg ttttgctgcg cgttatgccc
    10261 acctcggttt tcgtattgcc gatgaaactc tgacgctgat gcgcgagatg acccatgcgg
    10321 gtgaactgga acacctgacg cctgaacggg tatggaaaga gacggaaagc gccctcacca
    10381 cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg cggtgcactt cgcgttttat
    10441 tcccggaaat tgacgccctg tttggcgttc cggcccctgc caggtggcat ccggaaatcg
    10501 acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt ccgcaggttg
    10561 atgtccgttt cgcaacttta tgccacgatc tcggtaaagg actgacgccg ccagaacttt
    10621 ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt agtggaacaa ctatgccagc
    10681 gtctgcgcgt gccaaatgaa attcgcgatt tagccagact ggtggctgag tttcacgatc
    10741 tcatccacac cttcccaatg ctgaacccaa aaaccatcgt caaattattt gattccatcg
    10801 atgcctggcg taaaccgcag cgtgtcgagc aactggcgct gaccagcgag gctgacgtac
    10861 gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgttggttg cgcgaagcct
    10921 gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga agcgggattt aaaggtgtgg
    10981 agattcgcga ggagctgacc cgacggcgga ttgcggcggt agccagttgg aaggaacaac
    11041 gttgcccaaa gcctgactga gtataaaact aaacgttaac acgaaccatc ccctcgcccc
    11101 ttcggggaga gg
//
LOCUS       DAZKGS010000140        11027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKGS010000140 DAZKGS010000000
VERSION     DAZKGS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..1876)
                     /gene="glmS"
                     /locus_tag="ACHOQY_003743"
     CDS             complement(47..1876)
                     /gene="glmS"
                     /locus_tag="ACHOQY_003743"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9679761.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2038..3408)
                     /gene="glmU"
                     /locus_tag="ACHOQY_003744"
     CDS             complement(2038..3408)
                     /gene="glmU"
                     /locus_tag="ACHOQY_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9679762.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3759..4178)
                     /gene="atpC"
                     /locus_tag="ACHOQY_003745"
     CDS             complement(3759..4178)
                     /gene="atpC"
                     /locus_tag="ACHOQY_003745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9679763.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4199..5581)
                     /gene="atpD"
                     /locus_tag="ACHOQY_003746"
     CDS             complement(4199..5581)
                     /gene="atpD"
                     /locus_tag="ACHOQY_003746"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9679764.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5608..6471)
                     /gene="atpG"
                     /locus_tag="ACHOQY_003747"
     CDS             complement(5608..6471)
                     /gene="atpG"
                     /locus_tag="ACHOQY_003747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9679765.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6522..8063)
                     /gene="atpA"
                     /locus_tag="ACHOQY_003748"
     CDS             complement(6522..8063)
                     /gene="atpA"
                     /locus_tag="ACHOQY_003748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9679766.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8076..8609)
                     /gene="atpH"
                     /locus_tag="ACHOQY_003749"
     CDS             complement(8076..8609)
                     /gene="atpH"
                     /locus_tag="ACHOQY_003749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9679767.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8624..9094)
                     /gene="atpF"
                     /locus_tag="ACHOQY_003750"
     CDS             complement(8624..9094)
                     /gene="atpF"
                     /locus_tag="ACHOQY_003750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9679768.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9156..9395)
                     /gene="atpE"
                     /locus_tag="ACHOQY_003751"
     CDS             complement(9156..9395)
                     /gene="atpE"
                     /locus_tag="ACHOQY_003751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9679769.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9442..10257)
                     /gene="atpB"
                     /locus_tag="ACHOQY_003752"
     CDS             complement(9442..10257)
                     /gene="atpB"
                     /locus_tag="ACHOQY_003752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9679770.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10266..10646)
                     /gene="atpI"
                     /locus_tag="ACHOQY_003753"
     CDS             complement(10266..10646)
                     /gene="atpI"
                     /locus_tag="ACHOQY_003753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9679771.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2636 a         3016 c         2728 g         2647 t
ORIGIN      
        1 aaaaaagggc caccggtcgg cagccctttt ttatctatga tagtgattac tcaaccgtaa
       61 ccgattttgc caggttacgc ggctggtcaa cgtcggtgcc tttgatcagc gcgacatggt
      121 aagccagcag ctgcagcgga acggtgtaga agatcggtgc aatcacctct tccacatgcg
      181 gcatctcgat gatgtgcatg ttatcgctac ttacaaaacc cgcatcctga tcggcgaaga
      241 catacaactg accgccacgc gcgcgaactt cttcaatgtt ggatttcagt ttttccaaca
      301 gttcgttgtt cggtgcaacg acgataaccg gcatatccgc gtcaatcagc gccagcgggc
      361 cgtgtttcag ctcgcctgca gcgtaggctt cagcgtgaat gtaagagatc tctttcagct
      421 tcaatgcgcc ttccagcgcg attgggtact gatcgccacg gcccaggaac agcgcgtgat
      481 gtttgtcaga gaaatcttct gccagagctt caatgcgttt gtcctgagac agcatctgct
      541 caatacggct cggcagcgcc tgcagaccat gcacaatgtc atgttcaatg gaggcatcca
      601 gacctttcag gcgagacagc ttcgccacca gcatcaacag cacagttaac tgagtggtga
      661 atgctttagt ggatgccacg ccgatttccg tacccgcgtt ggtcatcagc gccagatcgg
      721 attcgcgcac cagagaagaa cccggaacgt tacagattgc cagcgaacca aggtaaccca
      781 gctctttcga cagacgcagg cccgccaggg tatccgcggt ttcgccagac tgtgacaagg
      841 tgatcatcag gctgttacga cgcacggcag atttgcgata gcggaattca gaggcgattt
      901 cgacgtcgca cggaatacct gccagcgatt caaaccagta acgggaaacc ataccggagt
      961 tataagaagt accacaggcg aggatctgaa tatgctcaac cttcgacagc agttcgtcgg
     1021 cgttcggtcc cagctcgctt aaatcaacct gaccgtggct gatgcgcccg gtaagggtgt
     1081 ttttgatcgc gttcggctgt tcgtagatct ctttctgcat gtagtgacgg taaatacctt
     1141 tatcgcccgc gtcatattgc agattggatt cgatatcctg acgttttact tccgcgccag
     1201 ttttatcgaa gatgtttacc gaacggcgag taatttccgc aatatcgccc tcttcaagga
     1261 agataaagcg acgggtcacc ggcagcagcg ccagctggtc agaagcgata aagttttcgc
     1321 ccatacccag accaatcacc agcggactac cagaacgtgc cgccagcagg gtatccgggt
     1381 gacgggagtc catgatcact gtaccgtacg caccacgcag ttgcgggata gcacgcagaa
     1441 cagcctcacg cagagtaccg ccctgtttca gctcccagtt caccagatgg gcaattactt
     1501 cggtgtctgt ttcagaaacg aaggtatagc cacgcgcttt taactcttcg cgcagcggtt
     1561 catggttttc gatgatgccg ttatgcacca ccacaatgtg ttcagaaaca tgcggatgcg
     1621 cattcacttc tgaaggttca ccgtgggtcg cccagcgagt atgagcaata cccgtaccgc
     1681 catgcagagg atgttcttcc gctgcctgag ccagcatctg gactttaccg aggcgacgca
     1741 ggcgggtcat atgaccttcc gcatcaacaa cggccagacc ggcagagtca tatccgcggt
     1801 attccagacg acgtaaacct tcaagaagga tttctgctac atcacgttgc gcgatcgcgc
     1861 caacaattcc acacatagtt tttgattccg atttatatcg ttgtcggtca acctgtatgc
     1921 ccgttttttc gggcgccccg agccttgtag agagtggggt tatttttata gttactgctt
     1981 gtgggaggga gattatgtta tctcctcatc ccatgtgacc ggattacccg gccagaatca
     2041 ctttttcttt accggacgac gccagccttc tttctgagtc tgcggcacac ggctgatagc
     2101 taatgcattt tcgccgacat tacgcgtcac agttgtaccc gcagcaatgg tcgcgccttt
     2161 gcctactgtt accggggcca ccagctgagt gtcggaaccg acaaacacat cgtcgccgat
     2221 aatggtctta aatttattcg caccatcgta gttgcaggta atggttcccg cgccgatgtt
     2281 aacgttatcg ccaatttccg catcgcccag gtaagtcaga tgaccagctt tcgagccttt
     2341 acccagacgc gcttttttca tctcaacaaa gttaccgacg tgtgcacctt ccagcaactc
     2401 agcaccagga cgcagtcggg caaacgggcc aatagtacag gccgctgcca gattcgcgtc
     2461 ttccacaacg gtatacggac tgatttcgca atcatcacca atcacgctgt ttttaatcac
     2521 gcaaccggtg ccgattttca cgcgatgacc gagagtcacg ttgccctcga tgataacgtt
     2581 agtatcaatt tcaacatcgc gcccgtgagt aagcgtaccg cgcaaatcaa aacgcgccgg
     2641 atcgcgcagc ataacgcctg ctaacagcag tttttcagcc tgttcggact ggtaaacgcg
     2701 ctccagacgg gagagttgca ggcggttatt cacaccttct acttcgctta aacgttgcgg
     2761 atgaacggcg acgatttcac gcccttcctg atacgccagc gcaataatgt cggtgatgta
     2821 gtattcgccc tgagcattat tgttggtcag cttcgccagc cagcgtttca tatctgcgcc
     2881 gttagcgatc agaatgccgg tgttgatctc ctgaatctga cgctgctcgt cggtggcatc
     2941 tttgtgctca acaatgccgg taactttgcc attttcacgg gtgatacgtc cataaccggt
     3001 cggatcatcc agtttcaccg tcagcagacc aatgccaccc tgcggtttag catcacgcag
     3061 gcgctggagt gtttcgacag agatcagcgg cacgtcgccg tagagcatta aaatgtcttc
     3121 atcatcggca aagaaaggtg cggcctgctg cattgcatga cccgtaccca gctgctctgc
     3181 ctgaagcacc cagttaaggt tgtcgtcttt cagcgcctgt ttaagcagat cgccgccgtg
     3241 accgtacacc aggtgaacgt gcgctgcgcc taattcattc gcagcatcaa tgacatgctg
     3301 aaccatcgct ttcccggcaa gggtatgcag cactttcgga agatcggaat acatgcgcgt
     3361 gcctttgcct gcggcaagga tcactacgct catagcatta ttcaacatac gcgtcctgac
     3421 tgtaatttga gaacgaattt aaaccgcttc accttgaaaa aactacattt ttttcatcgt
     3481 gaaatggaca gaggataaat tgttcaataa cggattatcc ccgctgacga aagtgccatt
     3541 ttcgaccatc gtcacgcctt ttgtctgcag aaaattaagg cagtaccttc atttaagcgt
     3601 caggtggatg tttttgctct tatttcgatc aatgaataaa cgggaaaaca ggggtgtttt
     3661 atatcagcag gatctatgtg aacgctcttc aggacggatc acgcgtaaaa aaaagccagc
     3721 ctgtttccag actggctttt gtgcttttca agccggtgtt acatcgcttt tttggtcaac
     3781 tcgataacgc gcagctgcgc gatcgctttg gccagttccg cagacgcctg agcgtaatct
     3841 acgtcgccgt gagagctgct aatgtgctct tcagccttac gtttcgcttc catggctcgc
     3901 gcttcgtcga gatcctggcc gcgaattgcg gtgtcggcca gaacggtcac gttgccaggc
     3961 tgcacttcaa gaatgccgcc agacagatag ataaactctt cgtgaccgtg ctgtttcacg
     4021 atgcgaatca taccaggctt aatggcggtg agcagcggtg cgtggccagg gtagatcccc
     4081 agttcacctt cgctacccgt tacctggatt ttctcgacca gaccagagaa catttgttgc
     4141 tctgcgctga cgacgtccag gtggtaagtc attgccatat caccctccga ttaaggcgtt
     4201 aaagtttttt ggctttttcc acagcttctt cgatggaacc gaccatgtag aacgcctgct
     4261 ccggcaggtg atcgtattcg ccttccatga tgcctttaaa gccacggatg gtgtctttca
     4321 gggagacgta tttacccgga gaaccggtga atacttctgc cacgaagaac ggctgggaca
     4381 ggaagcgctg gatcttacga gcacgcgcta ccaccagttt gtcttcttca gacagttcat
     4441 ccatacccag gatggcgatg atgtctttca gttcctgata acgttgcagg atggactgaa
     4501 cgccacgcgc ggtgtcgtag tgttcctgac caaccaccag cgggtccagc tgacggctgg
     4561 tggagtccag cgggtcaacg gccgggtaga tacccagaga cgcgatctga cggctcagta
     4621 ccacggttgc gtcaaggtgc gcaaaggtgg ttgccggaga cggatcagtc aagtcatccg
     4681 caggtacgta taccgcctgt acggaggtga tagaaccagt tttggtggag gtgatacgtt
     4741 cctgcagaac gcccatctct tccgccaggg tcggctgata acctaccgct gaaggcatac
     4801 ggcccagcag tgcggatact tccgtaccgg ccagggtgta acgatagatg ttgtcaacga
     4861 acagcagaac gtcacgacct tcgtcacgga atttctcagc catggtcaga ccggtcagag
     4921 caacgcgcag acggtttccc ggcggctcgt tcatctggcc atacaccagg gatactttgt
     4981 cgataacgtt ggagtcggtc atttcgtggt agaagtcgtt accctcacga gtacgttcac
     5041 ctacgcccgc aaacacagag taaccggagt gctcgatcgc gatgttacga atgagctcca
     5101 tcatgtttac ggttttacct acacccgcac caccgaacag accaacttta ccgcccttag
     5161 cgaacggaca catcaggtcg ataactttga taccggtttc cagcagttcc tgagagtttg
     5221 acagctcttc gtaggaaggt gctgcgcggt gaatcgccca acgctcttct tcaccgatct
     5281 cgcctttcat gtcgaccggt tcacccagta cgttcatgat acggcccaga gtcgctttac
     5341 ctaccgggac ttcgatcggg tgttcgaggt cttttacatc cagaccgcga cgcagaccgt
     5401 cggaggaacc cattgcgatg gtacgcacga taccgccgcc gagctgctgc tgaacttcca
     5461 gcaccagacg ctcattacca ttttgcacct caagagcatc gtacacgcgc ggtacggcat
     5521 cctgagggaa ttcgacgtca actacggcgc cgattacctg gacaatcttt ccagtagcca
     5581 tcttaaatcc tctacgaaat aacctgttta aaccgcggcg gcccccgaga cgatctcggt
     5641 gagttcctga gtaatgctgg cctgacgagc tttgttgtat accaactgca gctctttaat
     5701 caggctgccg ccattgtcgg tcgcggcttt catcgccacc atacgggcgg cctgctcgct
     5761 ggccaggttt tcaaccacgc cctgataaac ctgagattcg acataacgac gcagcagggt
     5821 atccagcaac gccttcggat cgggttcgta caggtaatcc caggatttat gtttcagatc
     5881 atcatcatct gatgccggta acggcagcag ctggctgatg gtcggaacct gagacatggt
     5941 gttaataaat ttgttgctga caatgtaaag cttgtccaga cggccttcgt cgtaggcctg
     6001 caacatcact tttaccggac cgatcagttc ggacagggaa gggttatccc ccatgccggt
     6061 gacctgggca acaacattgc cgcccacgga gttgaagaac gacacgcctt tcgagccgat
     6121 cattgcgagg tcgcattgaa cgcctttgtc ggtccaggtc ttcatttccg ccagcagttt
     6181 tttgaacagg ttaatgttca aaccaccgca caaaccacgg tcggtcgaca ccaccaggta
     6241 gcccacgcgt ttaacgtcgc gttcttccag gtatgggtgc ttatattcca gattaccgct
     6301 cgcaaggtga ccaatcactt tgcgcatggt atctgcataa ggacggctgg ccgccatgcg
     6361 atcctgcgat ttacgcattt tggaagcggc gaccatctcc atcgctttag tgatcttttg
     6421 cgtgttctgg acgcttgcga tcttactacg tatctctttt gcgccggcca tgagcttctc
     6481 ctcaatgcct tgcggcctgc cctaaggcaa gccgccagac gttaccagga ttgggttgct
     6541 ttgaaggaat cgaggatgcc tttcagcttg ccttcgattt cgtcgttgta gccaccggtc
     6601 tggttgatct cttgcatcaa cggagcgtga tcacggtcga cgtaagccag cagagcggct
     6661 tcgaagctgc cgattttcga cagttcaaca tccgccaggt aaccacgttc tgctgcgaac
     6721 agaaccagag actgttgcgc tacggacatc ggcgcatact gtttctgttt cagcagttcg
     6781 gtcactttct gaccgtggtc aagctgctta cgtgttgcat cgtcaaggtc ggatgcaaac
     6841 tgagagaacg ctgccagttc acgatactgt gccagagcgg tacggatacc accggacagt
     6901 tttttcatga tcttggtctg tgctgcacca ccaacacggg atacggaaat acccgggtta
     6961 accgcaggac gaataccggc gttgaacagg ttggtttcca ggaagatctg accatcggta
     7021 atggagatta cgttggtcgg aacgaacgca gaaacgtcac ccgcctgagt ttcaataatc
     7081 ggcagtgcgg tcagagaacc ggttttccct ttcacttcac ctttggtgaa ggcttcaacg
     7141 tattcggcgt taacacgcgc agcacgctcc agcagacgag agtggaggta gaatacgtcg
     7201 cccgggaatg cttcacgtcc tggcggacga cggagcagca gggagatctg acggtaagca
     7261 acagcctgtt tagacaggtc atcgtaaatg atcagcgcat cttcaccgcg gtcacggaag
     7321 tattcgccca ttgcgcaacc ggcatacgga gccagatatt gcagtgcagc ggattcagac
     7381 gcggttgcta ccacaacgat ggtgttagcc agtgcgccgt gctcttccag tttacgtacc
     7441 acgttagaaa tggtggacgc tttctggccg atagcgacat agatacattt gataccggaa
     7501 tcgcgctggt tgatgatggc atcgatagcc agtgcggttt taccggtctg acggtcaccg
     7561 atgatcaatt cacgctgacc acgaccaatt gggatcatgg agtcaacggc tttataaccg
     7621 gtctgtaccg gctgatctac ggactgacgt tcgataacgc ccggagcgat tgcttctaca
     7681 gcagagaagc cgtcgtgatc cagcggacct ttaccgtcga ttggtgcacc cagagtgtta
     7741 accacacggc ccagcaggcc acggccaacc ggaacttcca ggatacggcc agtacactta
     7801 actttcatgc cttcggcaag gtcagcgtac ggacccataa caaccgcacc tacagagtcg
     7861 cgctcgaggt tcagtgcgat agcgtaacgg ttacccggca gggagatcat ttcaccctgc
     7921 atacaatcgg ccaggccgtg aatgcggata acaccgtcac ttacagaaac aatagtacct
     7981 tcgttgtgag cttcactcac aacattgaac tgagcaatgc gctgcttgat cagttcgctg
     8041 atttcggtgg aattcagttg catgctccag tccccttaag actgcaagac gtctgcaagg
     8101 cgctcaagac gaccgcgtac gctgccatca atgaccatat cacccgctcg gatgataacg
     8161 cctgccatta cagacttatc gattttgcaa ttcagcttaa ctttgcgtga cagacgtttt
     8221 tccatcgcag cagaaatttt cgcgagctgt tgttcactca gtgcggcagc ggaaatgacg
     8281 tctacctcag cggtagcctc actcacggca cgaaggtgaa taaactgctc cagaacatcc
     8341 gggagcgcgt taagacgacc attttcagcc attacccgaa tcaggttctg accgttttcg
     8401 tccagttgct caccacaaac tgcgataaac gactcggcga gcgtttctgg cgcaagcgcg
     8461 ccagagagaa gctctgccat ttgttcgttt ttggttacct cggcggcaaa cgccagcatg
     8521 tcctgccagc gttctacact ttggtgttcg acggcaaagt caaaagctgc tttggcgtag
     8581 gggcgagcta ccgtaataaa ttcagacatc agcccctccc tccttacagt tcagcgacaa
     8641 gtttatccac gatgtcgctg ttagcagctt catccacgga acgttcgatg atcttctcgg
     8701 cgccagcaac agccaggata gcaacttgct tacgcagctc ttcacgggca cgtttacgct
     8761 cggcttcaat ttctgcctgc gcctgggcca cgattttagt acgttcctgt tctgcctcag
     8821 ctttcgcttc gtccagaatc tgcgagcggc gtttgttcgc ctgctcgatg attacctggg
     8881 cttccgcttt cgcttttttc agctggtcgg tcgcgctggc ctttgcaagg tcaaggtcct
     8941 tatgtgctcg ttctgcggaa gcaaggccgt cagcaatttc tttttgacgt ttttcgatgg
     9001 ctgccattaa tggcggccat acgtacttca tgcagaacag aacgaacagg acaaacgcga
     9061 tggcctggcc gaggattgtt gcgttaagat tcacagcaca atgcctctat ttagttaacg
     9121 ttctgatatt gctctttaaa taaaagcaac gcttactacg cgacagcgaa catcacgtac
     9181 agacccagac ctacagcgat catcgggata gcatccacca gacccataac gataaagaac
     9241 tgagtacgca gcagaggaat cagatcaggt tgacgcgctg cgccttccag gaatttaccc
     9301 ccgaggatgc cgataccgat cgcagcaccg attgccgcca gacccatcat cacagcggca
     9361 gccatgtaca gcagatccat attcaggttt tccatgacag tctccagttt gtttcagtta
     9421 aaacgtagta gtgttggtaa attaatgttc ttcagacgcc atcgacagat agacgatcgt
     9481 cagaaccatg aagatgaagg cttgcagcgt aatgatcagg atgtggaaaa tggcccacgg
     9541 cacattcagg atccactgtg accaccacgg caacagacca gcaatcagaa tgaaaatcag
     9601 ctcaccggca tacatgttac cgaacagtcg caaaccaagt gaaactggtt tggacagcag
     9661 gcttacccct tcaaggatta agttgacagg aatgaacgcc cagtgattga acggctgcag
     9721 cgtcaactct ttcgtgaagc cgccgatgcc tttcattttg atgctgtaga acagaatcag
     9781 gataaatacg cccaatgcca tagacagcgt tacgttcacg tccgcagacg gaaccacacg
     9841 cagtgcaggc agacccagta catgttcagc aatgtacggc agcaggtcga taggcagtaa
     9901 atccatcagg ttcatcagga atacccagac gaagatcgtc agggccagcg gagcaatcag
     9961 cttgcttttg ccatggtaca tgtctttcac gctaccatta acaaagccga tcaccagctc
    10021 aatcgcggtc tgaaacttac ctggcacacc gctggtcgcc tttttggcta cgctacggaa
    10081 taaaaccagg aacaacagac ccagcaccac cgagaagaac atggagtcaa tattgattgt
    10141 ccagaaggtg gctggggggt tttgtggatc caccagcgag aatgtacgca ggtccagctg
    10201 aaggttattc aggtggtgtc ctatgtaatc ctgcggcgtc atattttctg aagccatgat
    10261 gccttttacc ctttgttgtt aattacagcc ggtgccagta tctgaaccac cagcaccaaa
    10321 acccacgtaa cgatcagcgg caagaatacc gcctttaaaa ccgccaacgc caccaccagt
    10381 aacaccaaca tcgccagaac tttgaaagct tcgccaaatg cgaatgtcca ggccacccgg
    10441 cctttcgctg gtgtatgcgc ctggtgacgc caggcaaata tcataaacaa aacgttaggc
    10501 agaaagactg ccaggccccc gcttattgca gagacgcccc agaaggggtc tttgaggctg
    10561 aacagcaatc cacttgctat caccaccagt aactgaacga gcagaagctt ccgagcaacg
    10621 tttcgactca cgagcgacac agacatcacg tttttcactc ctgctccctt cgaggtatgc
    10681 cgcgtgtcgt ataaaacttt ctttaaggct tagagtcaag catcaaaaag cggtcaaatt
    10741 atacggtgcg cccccgtgat ttcaaacaat aagtagccaa aaggtgaata aatgtttaaa
    10801 tatttttcca gtgcatacaa ttgcgacttt tctgctaacc ctgttcgatc atgaaaaact
    10861 gtaaataacg cgtaaacact ggtgataaag cgtgcttcag atcacatatt gcgcatgttc
    10921 gcgcacagca tatttattta cttggcaaat gatgcctttg caagtttatg atatttcagt
    10981 ctaaaaacag atactgtttt aataaatgac atttacacaa caaaaac
//
LOCUS       DAZKGS010000141        10945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKGS010000141 DAZKGS010000000
VERSION     DAZKGS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            179..829
                     /gene="csgD"
                     /locus_tag="ACHOQY_003754"
     CDS             179..829
                     /gene="csgD"
                     /locus_tag="ACHOQY_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9679772.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            834..1223
                     /gene="csgE"
                     /locus_tag="ACHOQY_003755"
     CDS             834..1223
                     /gene="csgE"
                     /locus_tag="ACHOQY_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9679773.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1248..1664
                     /gene="csgF"
                     /locus_tag="ACHOQY_003756"
     CDS             1248..1664
                     /gene="csgF"
                     /locus_tag="ACHOQY_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9679774.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1691..2524
                     /gene="csgG"
                     /locus_tag="ACHOQY_003757"
     CDS             1691..2524
                     /gene="csgG"
                     /locus_tag="ACHOQY_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9679775.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2588..3079)
                     /gene="ycdZ"
                     /locus_tag="ACHOQY_003758"
     CDS             complement(2588..3079)
                     /gene="ycdZ"
                     /locus_tag="ACHOQY_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9679776.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3181..3735)
                     /gene="ycdY"
                     /locus_tag="ACHOQY_003759"
     CDS             complement(3181..3735)
                     /gene="ycdY"
                     /locus_tag="ACHOQY_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9679777.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3759..4496)
                     /gene="ycdX"
                     /locus_tag="ACHOQY_003760"
     CDS             complement(3759..4496)
                     /gene="ycdX"
                     /locus_tag="ACHOQY_003760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9679778.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4551..5489)
                     /gene="ghrA"
                     /locus_tag="ACHOQY_003761"
     CDS             complement(4551..5489)
                     /gene="ghrA"
                     /locus_tag="ACHOQY_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9679779.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5723..5810
                     /locus_tag="ACHOQY_003762"
     tRNA            5723..5810
                     /locus_tag="ACHOQY_003762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5757..5759,aa:Ser,seq:gga)
     gene            complement(5960..6802)
                     /locus_tag="ACHOQY_003763"
     CDS             complement(5960..6802)
                     /locus_tag="ACHOQY_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9679780.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6887..7084)
                     /locus_tag="ACHOQY_003764"
     CDS             complement(6887..7084)
                     /locus_tag="ACHOQY_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9679781.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7096..7587)
                     /locus_tag="ACHOQY_003765"
     CDS             complement(7096..7587)
                     /locus_tag="ACHOQY_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9679782.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7584..7958)
                     /locus_tag="ACHOQY_003766"
     CDS             complement(7584..7958)
                     /locus_tag="ACHOQY_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9679783.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8005..8379)
                     /locus_tag="ACHOQY_003767"
     CDS             complement(8005..8379)
                     /locus_tag="ACHOQY_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9679784.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8542..8763)
                     /locus_tag="ACHOQY_003768"
     CDS             complement(8542..8763)
                     /locus_tag="ACHOQY_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9679785.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8826..9302)
                     /locus_tag="ACHOQY_003769"
     CDS             complement(8826..9302)
                     /locus_tag="ACHOQY_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9679786.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9318..9803)
                     /locus_tag="ACHOQY_003770"
     CDS             complement(9318..9803)
                     /locus_tag="ACHOQY_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9679787.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9894..10712)
                     /locus_tag="ACHOQY_003771"
     CDS             complement(9894..10712)
                     /locus_tag="ACHOQY_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9679788.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTIADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2766 a         2726 c         2727 g         2726 t
ORIGIN      
        1 atttaagatt tgtaatggct agattgaaat cagatgtaat ccattagttt tatattttac
       61 ccatttaggg ctgatttatt actacacaca gcagtgcaac atctgtcagt acttctggtg
      121 cttctatttt agaggcagct gtcaggtgtg cgatcaataa aaaaagcggg gtttcatcat
      181 gtttaatgaa gtccatagta ttcatggtca tacattattg ttgatcacta aaccgtcttt
      241 gcaagcgaca gctctcttgc agcaccttaa acaatcgctg gcaattacag gaaaattaca
      301 taatattcaa cgttctctgg acgatatatc ttcaggctct attattcttc tggatatgat
      361 ggaagcagat aaaaagctta tccattattg gcaggatact ttgagcagga aaaacaacaa
      421 tatcaaaata ttgttgctaa atacgcctga agattacccg taccgcgaca ttgaaaactg
      481 gcctcatatc aacggcgttt tttatgccat ggaggatcaa gaacgtgttg tcaatgggtt
      541 gcaaggcgtc ctgcgcggcg aatgctactt tacgcaaaag cttgccagtt acctgattac
      601 acattctggt aactatcgtt ataacagcac ggaatcagcc ctccttactc atcgggaaaa
      661 agagatcctg aataagctgc gtatcggcgc gtctaataac gagatcgctc gttcgttgtt
      721 catcagcgaa aatacggtta aaacgcatct ttataatctt ttcaagaaga tagccgtaaa
      781 aaaccggaca caagcggttt cctgggcaaa cgataacctc aggcgataaa gccatgaaac
      841 gttatttacg ctggattgtg gcggcagaat ttctgttcgc cgcagggaat cttcacgccg
      901 ttgaggtaga agtcccggga ttgttaactg accatactgt ttcatctatt ggccatgatt
      961 tttaccgagc ctttagtgat aaatgggaaa gtgactatac gggtaactta acgattaatg
     1021 aaaggcccag tgcacgatgg ggaagctgga tcactataac ggtcaatcag gacgttattt
     1081 tccagacttt tttatttccg ttgaaaagag acttcgagaa aactgtcgtc tttgcactga
     1141 ttcaaactga agaagcacta aatcgtcgcc agataaatca ggcgttatta agtacgggcg
     1201 atttggcgca tgatgaattc taaataaaaa attgttcgga ggctgcaatg cgtgtcaaac
     1261 atgcagtagt tctactcatg cttatttcgc cattaagttg ggctggaacc atgactttcc
     1321 agttccgtaa tccaaacttt ggtggtaacc caaataatgg cgctttttta ttaaatagcg
     1381 ctcaggccca aaactcttat aaagatccga gctataacga tgactttggt attgaaacac
     1441 cctcagcgtt agataacttt actcaggcca tccagtcaca aattttaggt gggctactgt
     1501 cgaatattaa taccggtaaa ccgggccgca tggtgaccaa cgattatatt gttgatattg
     1561 ctaaccgcga tggtcaattg cagttgaacg tgacagatcg taaaaccgga caaacctcga
     1621 ccatccaggt ttcgggttta caaaataact caaccgattt ttaagcccca gcttcataag
     1681 gaaaataatc atgcagcgct tatttctttt ggttgccgtc atgttactga gcggatgctt
     1741 aaccgccccg cctaaagaag ccgccagacc gacattaatg cctcgagctc agagctacaa
     1801 agatttgacc catctgccag cgccgacggg taagatcttt gtttcggtat acaacattca
     1861 ggacgaaacc gggcaattta aaccctaccc ggcaagtaac ttctccactg ctgttccgca
     1921 aagcgccacg gcaatgctgg tcacggcatt gaaagattct cgctggttta taccgctgga
     1981 gcgccagggc ttacaaaacc tgcttaacga gcgcaagatt attcgtgcgg cacaagaaaa
     2041 cggcacggtt gccattaata accgaatccc gctgcaatct ttaacggcgg caaatattat
     2101 ggttgaaggt tcgattatcg gttatgaaag caacgttaaa tcgggcggtg ttggggcaag
     2161 atattttggg atcggtgcag acacgcaata ccagctcgat cagattgccg tgaacctgcg
     2221 cgtcgtcaat gtgagtaccg gtgagatcct ctcctcggtg aacaccagta agacgatact
     2281 ttcctatgaa gttcaggccg gggttttccg ctttattgac taccagcgct tgctggaagg
     2341 ggaagtgggt tacacctcaa acgaacccgt tatgctgtgt ctgatgtcgg ctatcgaaac
     2401 aggggtcatt ttcctgatta atgatggtat cgaccgtggt ctgtgggatt tgcagaacaa
     2461 agcagaacgg cagaacgaca ttctggtgaa ataccgccat atgtcggttc caccggaatc
     2521 ctgacagata aaaaagccgg aattatcatc cggcatttta ctgtttaagc gaaagcgtga
     2581 gtgcccctca cgctttattt ttgatttcct gctcacggtg agccgtcttt gcactacgcg
     2641 ccgccagcca caggccgctg tttttcattg cgtaaccaaa taccagccca agcgccagcg
     2701 aaggtaacac cagtttccag tcaccctgcc cggcaaacgt cgcgcatgcg cctataaacg
     2761 tccccggaac aaatgaaagc agcagctgtt tggcctgaat acacatcaga aacgcgacaa
     2821 taccggttat gacataaccg agaatttcca gatgtggtgc cagtgcacta ccgtaaataa
     2881 tgaccatcgc ccacaccacg ccactaagca gggttgcagc ggagatcgcc agccctttca
     2941 gcccaccttg cgggcaggca aagtaggccg tacagcccag gaagcccgcc cagcttagta
     3001 agcccaggga aacagccacc catccccaga taccggagag aatgcctgtt gtgattgcaa
     3061 tagagagaag tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg aatgagatcg
     3121 agcacacatt ttatgcaaca acaaattatc tgttgcagtt atgagtgacg atgatcacat
     3181 ttactcttca gaatcttctt ccagctcgtc ccacattgca ctaatggcat cacgggttag
     3241 cggtgccatg gtgcgccaga aaggcgtggt tgcatgggcc tccactttgc cgaggaacgc
     3301 accacaccag ggtaacagat actcactgaa cagtgtttcc agtgcttcgc tctcatcttc
     3361 cgttgactga tcttccagcc aggaagccgc aagcagcaat gtgccgatgt gatcggctgg
     3421 cgtatccgcc agtggcatcc ctcgttcgga aagaaaagcg cgcacttccg cttccgttgc
     3481 gtcctcaacc catgcgctac gatatggcgg cacggcacat tcatcgccga taaacaacgc
     3541 attgtaatcg gcagagactt gcgccatatc acagcttttc tgtaaacgcg tcagtaactc
     3601 atcctgctcc agtggccagt tcgcagccag tttcccctca cgaatcaggg taaacagcgg
     3661 caccagtaaa ggatcttgcg gttggcggta atagagcgaa cccagcacac gacagaggat
     3721 agaaaactcg ttcattaata tatttccatt actagtaatt aaaggtctgc aaattccgca
     3781 atcggtgcca taccgcgaga ttcaaggaag ttcagtaagc ggcgcggcga aacattcaaa
     3841 atgcgctctg gcggaaaatc taccgcgtcg aggattttaa gacactcttc aaattccccc
     3901 atggtaaacg cggtgtgaga atccgagcct aatgccaccc aacctccagc atcacgtacc
     3961 gctgcggcta ccgcacgaca gttgtcttca ctgcccttac gtgagtgtaa aaatgaagaa
     4021 ttattgattt ccagcgccac ctgatgtttc gcggctgctt cagcaacggc tttcacatct
     4081 atttcatatt tgggatttcc gggatggctt attatatgca cattgccgct ggcgatagtg
     4141 gcgatcatcg cttgtgtatt ggtcgctttg tcatgtggtg caaaaaccgg ctcatgaaaa
     4201 ccggcaataa tcagatccag cgagtcaaac attttaccgc tgcaatcaat ttcaccatca
     4261 acatttttaa tgttagcttc gatgccgcgc aggatcccta ccccatcaac cactcgcggc
     4321 cagatacgca tgttaatgaa gtgccagtga tgcggagcat cttccatatc cgggccatga
     4381 tcggtgatcg caaaaagttt aatgcccttt tgtttggcct gggcaatgta atcactcaat
     4441 gtgctatatg catgtgtgct ggcaacggta tgcatatgaa ggtcgacggg atacataact
     4501 ctctcctgta ttcaattccc gccaaggata gcaggaatcc tgatgcttta ttagtagccg
     4561 cgtgcgcggt cgacttgccc gcagaccctc tccccttttt cgagctgggc aatggtgcga
     4621 gaaatgtact ccacagcttc agcgggacgg gtaatcgcgg cgacatgtgg tgttatcgtc
     4681 acgcgtggat gttgccagag cggactttca ggcggtaagg gttcacgatt aaaaacatcc
     4741 aacattgcgc ctttaacttt gccgctatcc agcgccgcga gcaggtcatc ttccacaaca
     4801 tgaacaccac gcgccaggtt gaggagatac gcgccatccg gtaatttttc gagtaattgt
     4861 tgattaataa tgccgacggt ttcaggggta ttcggtaaca aattaatcaa tacccgacat
     4921 tggctcagaa atgcagacag ttcttcccgt ccggcaaagc tttgcacgcc aggccacgat
     4981 ttacgggttc gactccagca acgcagcgga aagcgccagg tttgcagact ctgagcaact
     5041 ttactgccca gtacgcctgc gcccaaaatg ccgatggtaa aatcttcccg atgatattca
     5101 ggcagcggtt gccaatgcga actattttgc tggatgcgat aatcgtcaaa acgtcgaaac
     5161 caatgcagca cctgactgac agcatattcc tgcatttgct cgcccatacc ggtatcttcc
     5221 aggcgaaaaa gtggaacaga agggttcagc atttcagggt gtgcctgtag cttgctcaaa
     5281 atagaatcaa caccggcccc gagtgcgaac accgctttaa gatcgcgccc tgccagcatt
     5341 tcaacaggag gatgccagac taaagcataa tcagcagagt cattatctcc gcttttccac
     5401 gctctgactc ttgcctgagg aatagctttg cgcagtgcct caatccacca ttgggtatcg
     5461 aacgttgggt gataaaagat gatatccata ctgactcccg aaaagcgttg tgcgaaattt
     5521 attcgcactt atcgttatga tctacaaagg ccaccagcat aacaaatccg tggtcggtgg
     5581 caaaaaaagc agatttcgct tattaaaacc acacattgat tgaagtttga ataaacgcgc
     5641 gattttttca aaaagtttgt tgacctcagg tcatgatttc cctaaattag cgcccgttcc
     5701 agcaagacag gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa
     5761 agtgtgtata cggcaacgta tcgggggttc gaatcccccc ctcaccgcca gattataagc
     5821 gcatgttatt gtttaacatg cgctttttta ttttcccttc cagacagtat gccatcgggt
     5881 atgccatctg aaaccggttt atatgccccg cgatcatatc ttatcggaca atttccgatc
     5941 tttgagcata ctggcaaagc tatactgcca gcatatcagg atagtgtttt gcgataatat
     6001 cattcagtct gtcaaccagt tctggttttc tgaacgtgat atgcgcggag cccttcttaa
     6061 agtatctgat gctgaacatc tcatcttcat agctctcttt accctgaaca gactgaatgt
     6121 gatcatccag acgaagggtg atgtttcccc ggttgtcggg gattggttta ccgctgaaca
     6181 ggtgcaacat tctttccaga tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa
     6241 atccccaccg gtcccatctc accagattgt tgacgataat tttactgcca aatttacagg
     6301 ggcaattggt tttgtaattc cagttcagcc ctctgaagac gttgatcacc cctcgctcaa
     6361 acacctcatc cttattctgg tgcagttgtt caaatgtgct cagaatgttc gcttcgctga
     6421 tttccggaaa attatcgtac tccagtgacc ggtaccatgt atcacgggcc tgcgtatcca
     6481 ttaatgacag catcccggaa cgttgcatca ggtcacgcca gatttcgcga tcaagattcc
     6541 gggtaatggc tttcattgct gtttcgggtt tctccatcag ccagcagccg cagcgaaaat
     6601 cctgtttcat ggcccagtcc ggggcagttt tacctccgat gctttccgtc agcaccgaga
     6661 tttcagccag ttgtcggata agtgtgtcga tttgcaacag tgtactcttt cgtacagcca
     6721 gaatgtggcg aatgctggtt gagcagataa cgtccgtatg cccgttgacc acttccggac
     6781 gggagattgt gatatctgac ataatcgata ttcctttttg cagataaatg aaaacgccag
     6841 ccctgtgcag gctggcgtca gaggaatgaa gtctggtgag tgagcttcac cggttctggt
     6901 gcagaagctg cagatgacgc agggtacgga catcctcccg ggcctgttcg atacagggca
     6961 acagtgctgc tgaatccgtt ttatcctcat cgttgtcaaa gataattccc gatccgcagt
     7021 cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg gttaatgatt
     7081 tcatgttgtt cctggttacc agtcgaaagt gggtaaaccc ggcaggcatt cagcgctggc
     7141 atccagatga atgagactga cgccataacg ccggatgagt gtgacgacca gacgacggaa
     7201 cgtttttgac agccccaggc gtttaagccg cagaaccggg tatgaccacg catcggtacg
     7261 taacagatat ccgctacctg taaaatgaat ccattctgat tcaccgaagt cactggtctg
     7321 gtgtgacagc gagtacaacc aggcgttgtc cgtttccgtg atatgtgcgg tactgcagcg
     7381 tatgccggta agggtagcaa atggtggcgt gcattctgct gaggtctggt cagattcatc
     7441 aacaacacga agggagtagc cgttatcgca gaccatgtta atcagttcag cgagctcaat
     7501 accatcgacg tcaacgacaa tgcgccccat gttcagtgcc tgtacgttaa cgctgtcggc
     7561 ttccagtgtc agggccagtt tcatcatttc gcctccggat acttacccaa ggtaatgtta
     7621 tttaccgttc tgtaattgtc gcgggtcatc aggccggtcg cccggcgagc ccggaggata
     7681 tcaatactgt ttattaactg agagcgggga caggtgctga atcccggctg gtcggtacgc
     7741 accagcgcgt atttttccac gagaaaatta actgcatcac acagtgaaat gcctgcctca
     7801 atatgctgct caatcacacg ttcatcggca aacggtgtgt cattcagtgt gaggccataa
     7861 tgctggtcca gcagtcggat gagcagtgtc tgccagatgg tgacgggaga cgggcagcga
     7921 gatacctccc gtacatgtgt gtcggataat gttttcatct taagttctct gtaactggta
     7981 gtgctaaagg tgagagcttc ggggttatga ggtggttgcg agtgctggtg tcggataaac
     8041 agccagataa acgtaaccac aggagccgag ggtgtcggct tcgcaggtca gcccttttgc
     8101 attcagcgtg acggtatgct gatatcgggg attcagttca ccgctggtga gcatgagttc
     8161 cagttgtttc atcagcagcg gaaaggcctg gtccagatgg tatgcatctg cgtcgctgaa
     8221 gcggcctctg ataccggcgc ggtcggcaag gtaatgcagg cggttaccct cttgcaccag
     8281 acgagcgcca aaacagggcc tcactgtgca gggaagcccc caccaggggc ggtcgtgatt
     8341 gtcatcggga tgagttatcc cggagagttt gtctgacacg ataaaatcct ctctgaaaag
     8401 tggttaagaa tggtttaacg atgacaggtc agccggcgcg ctgaccggtt tcggtgaaga
     8461 tgtatgcatt gatgagaatg gcctcatcca cagcggcgtc tgaggtcatc tggtcgtact
     8521 cgtttcccgg gtgacggaac atcagttcag ggtgacactc atcagccgga catacgggcc
     8581 aaaactgtcc ttacggcgtt ctgcaaacac ggcaagaacg ccgggaatat cctgcacttc
     8641 acgaccagta tacgcttcag cactgccgtg ccagcgatac ttaccggtac agaacggaaa
     8701 aagacgggat gtcggatgtt gttggtgaat acgcatggct tcaccacggg tgataatttt
     8761 cataatggga tacctctgaa gacagaagat aaaagtgaaa acaggtgtga tgtggttgtg
     8821 aagggttaaa gcagaccatg ttcggcaaag gagaaaacct ggttgccacc gactatcaga
     8881 tggtccggca cccggatatc caccaggccc agtgcctgta ccagacgttc ggtgataagc
     8941 cggtctgcct tactgggtgt gacttcaccg gacgggtgat tgtgcgccag caccacggca
     9001 gcggcattgt ggtacagggc gcgtttaatc acttcccggg gatgaacttc cgtacggttg
     9061 atggtgccgg tgaagagggt ttcaccggca atcagctgat tctggttgtt cagatacagc
     9121 acccggaact cttcccgctc cagtcccgcc atgttcagaa tcagccattc ccgtgccgca
     9181 cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt ttgcagggcc
     9241 cgcagaatga gactgcgctc gccgggcgtc atctctccgg gcagaaagga aagctgctgc
     9301 attgtccctg cctccattca gtcaatgatg cgcataatgg cgctgcattc cggatgctgc
     9361 agggcgtaat cccgcagccg gtaatagtgg accgtcatgg cataacactc cgtacgacag
     9421 gcatgatgac tgtacgtcat cagacaggca gcaataccgg cggcttccgg gctcatttca
     9481 gcgcggttgc cgttcatggc attgaacagt acccatgttt cgtcatcatc gtcatccggt
     9541 tcgggtgcca taaatgcccc gccgttgttc agggtgtaca gattccagat accaccgcag
     9601 tagtcttcgc aaagacggtc catccagccg aagacacggg gctccagggt cacccactgt
     9661 ggaatgaggc caaaatgctg cggccagaag ctgatgcgct gttcatcagg gacgggagtg
     9721 gcaaccagct gaggtgggtt attccctgat gcagtggtta cggaaacaga aggtgtggtg
     9781 gaattatgcg aaacggttgt catgaggtta ttccttataa aaagtaaatg aatggaagaa
     9841 gccccggggc aggaacagat gtgagtcagg actgcacttt cagtgaaacg gcatcagcgc
     9901 atgctctcca gcagcgtttc tgccatcacc cacaatgcgc gattgagctt aatgtcggta
     9961 tcaatgctgt gaatggcccg ggtatggata cgttttcctt ttgcactgcg accggaaatc
    10021 ccgcctttca gcatattctc ctggatggtc tgataagcac tccacaggtc cttaccgtaa
    10081 tcctcccggc gtcgtggtgt cagaatgtcg gcgatggtga cgggctgatg ttcgtcacca
    10141 taacggtaag tcagtgccgc ctgtgccagc gcctggcgtg ccggtggcgg caggaccagc
    10201 gactgcatgg catcacgctt ctcctctatc cggtcaaaaa cgcccaccac ctcgtaagcc
    10261 ccttcaatga ctctgtccac cacatttccc cggtgtggaa cacgcaattc tcccagagac
    10321 tgaccgcaga ccagtccatt ggtacaaatg gcacgaaaat atccgggtaa catctggtag
    10381 ctggatgaac cgtcatggga gttgagcaga ataatttcag ggacatgctg accggttatc
    10441 tgtccggccc gccgcagacg cagcatgtgt tttgtgtatt cccggcggct ctggtcgcgc
    10501 acgcgggtct ggcaggcaaa gaacggctga aagccttcct gctgcagatt ttccaggacg
    10561 gtgatagtgg gaatgtacgc atagtgttca ctgcgagagg tatgtcggtt ttccccaaaa
    10621 atactcggta catggcgcat cagttcttcg tgtgtcagcg gacgatcacg acgtatctgg
    10681 ttcgcataac caaaacgact ggctaatctc atgatttgct ccttatgaat gattaagcga
    10741 tataaatgaa aaagccgcgt ctcccggaga agacgcggcc tgatgggtga aatgaatgag
    10801 gtttattgtc tgagaagccc ttcactggcg aaccacgtat taagctgtat tccggcatcg
    10861 ccagtgcaac tgaccttcag cattgcggat aacccgccgg aaatatgttc actggttgtt
    10921 tttgtatctt cccccaaaat taaat
//
LOCUS       DAZKGS010000142        10857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKGS010000142 DAZKGS010000000
VERSION     DAZKGS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1109
                     /gene="yghO"
                     /locus_tag="ACHOQY_003772"
     CDS             <1..1109
                     /gene="yghO"
                     /locus_tag="ACHOQY_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP9679789.1"
                     /translation="VINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSAKNPGTD
                     HIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAALFGAAE
                     AWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHKGI
                     DLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGWQHNW
                     GFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSLFP
                     FGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRKIDAL
                     EMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1116..1880)
                     /gene="glcC"
                     /locus_tag="ACHOQY_003773"
     CDS             complement(1116..1880)
                     /gene="glcC"
                     /locus_tag="ACHOQY_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP9679790.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2131..3630
                     /gene="glcD"
                     /locus_tag="ACHOQY_003774"
     CDS             2131..3630
                     /gene="glcD"
                     /locus_tag="ACHOQY_003774"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP9679791.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3630..4682
                     /gene="glcE"
                     /locus_tag="ACHOQY_003775"
     CDS             3630..4682
                     /gene="glcE"
                     /locus_tag="ACHOQY_003775"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP9679792.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4693..5916
                     /gene="glcF"
                     /locus_tag="ACHOQY_003776"
     CDS             4693..5916
                     /gene="glcF"
                     /locus_tag="ACHOQY_003776"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP9679793.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5921..6325
                     /gene="glcG"
                     /locus_tag="ACHOQY_003777"
     CDS             5921..6325
                     /gene="glcG"
                     /locus_tag="ACHOQY_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP9679794.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6347..8518
                     /gene="glcB"
                     /locus_tag="ACHOQY_003778"
     CDS             6347..8518
                     /gene="glcB"
                     /locus_tag="ACHOQY_003778"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP9679795.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8746..8991
                     /gene="traT"
                     /locus_tag="ACHOQY_003779"
                     /pseudo
     CDS             8746..8991
                     /gene="traT"
                     /locus_tag="ACHOQY_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9024..10767)
                     /gene="cptA"
                     /locus_tag="ACHOQY_003780"
                     /pseudo
     CDS             complement(9024..10767)
                     /gene="cptA"
                     /locus_tag="ACHOQY_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
BASE COUNT         2723 a         2637 c         2957 g         2540 t
ORIGIN      
        1 aagtaattaa taaaaatgac cttaaggcat ttatcgcttt tccgtcatca ctttatcccg
       61 acgatccaaa ctggataccc cctttattca tagaacgcaa cgagcatttg tctgcgaaaa
      121 atccggggac ggaccatatc atctggcagg cgtgggtggc aaaaaaagcg gggcagatag
      181 ttgggcgtat taccgcgcaa atagatacct tgcaccgtga gcgttacggt gaagataccg
      241 gtcatttcgg catgattgac gccattgatg atccgcaggt ttttgccgcg ttgtttggcg
      301 cagcggaagc gtggttgaag tcacaaggtg caagtaagat cagcggtcct ttcagcctga
      361 atatcaatca ggaaagcgga ttactgattg aaggttttga cacaccaccc tgtgcgatga
      421 tgccacacgg caaaccgtgg tatgccgcgc atattgaaca actgggttat cacaaaggca
      481 ttgatttact ggcgtggtgg atgcagcgaa ccgatctcac tttctctccg gcgctaaaaa
      541 aactgatgga tcaggtgcgc aaaaaggtga ccattcgctg catcaatcgt cagcggtttg
      601 ccgaagaaat gcagatcctg cgtgagattt tcaactctgg ctggcagcac aactggggat
      661 ttgtgccgtt taccgaacat gaattcgcga ccatgggcga tcaacttaaa tatctggtgc
      721 cggacgatat gatctatatc gctgagattg attctgcacc ctgcgcgttt attgtcggct
      781 tgccgaacat caacgaggcg attgccgatc tgaacggatc gctctttccc ttcggctggg
      841 caaaattgct gtggcgtttg aaagtcagcg gtgtgcgaac tgcgcgagta ccgctgatgg
      901 gcgtgcgcga cgagtatcag ttcagccgca tcggcccggt gattgcgctg ttattgattg
      961 aagccttacg cgatccgttt gcccgccgga agatcgatgc gctggagatg tcatggatcc
     1021 tcgaaaccaa tacaggcatg aataacatgc tggaacgcat tggggcggag ccgtacaagc
     1081 gttaccgatt gtatgaaaag cagatttaat accagctaac tcaggttcat ctccagcggc
     1141 accgccgagc gaatcaaatg gtggccttcc agctcgatat cgtggagatt ctttttcacg
     1201 gtccgcacat gatcgcgtgc tgcgcgctgg gcgacgtgcg gcagccgctg caacaccgcg
     1261 ttgtagatcc tcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg atggtagaga
     1321 ttatttacgc tggcaaacac tgaattaaac atcagatcgg tcaatgattg cagcgtaaac
     1381 accagcacct gattgtgaga agcctgacaa atggcgagat ggaaagcgtg atccaactgc
     1441 gcatgttcga tcagcgaaat ctctttgttg ttctcactgg cggcgagcat tttttcataa
     1501 cagcgggtta tcacaacaaa atcagcctgc gttcccagcg ttgccgccag ccttgccgat
     1561 tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg ctgcgtactg
     1621 aacagatgga tcagcggact ggtgtcctgc acccgattaa gccgtgcgac acgagaatcg
     1681 cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc ttcacgcagt
     1741 gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag tggctgaccg
     1801 accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac ttcgcaaata
     1861 gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat ttttctgttc
     1921 ttaactcgca aaacacgcac atcacgtaag tgattatgtt acatcaattt aacattgagt
     1981 taaccaaggc aaggtcacag agctggaaaa aaaatggtct gaccggtagc taaagagata
     2041 gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg
     2101 ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat
     2161 ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct
     2221 ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc
     2281 gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg
     2341 attcttgctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg
     2401 ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa
     2461 gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccagg cgtgcgtaac
     2521 ctggcgatct cccaggccgt tgcaccgcat aatctctact acgcaccgga cccttcctca
     2581 caaatcgcct gttccattgg cggcaacgtg gcagaaaatg ccggcggcgt ccactgcctg
     2641 aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag
     2701 gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc
     2761 accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag
     2821 ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgca
     2881 gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg
     2941 tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt
     3001 ttgttatgcg agctggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac
     3061 gacatcttgt tgaaagcggg cgcgactgac gtccgtctgg cacaggacga agcagagcgc
     3121 gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat
     3181 tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt
     3241 gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc
     3301 aacatgcacc cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc ccgcgcggaa
     3361 gagctgggtg ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa
     3421 catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc
     3481 acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa
     3541 aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat
     3601 ttacctttcc ctgaactgga gcgtttctga tgctacgcga gtgtgattac agccaggcgc
     3661 tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca
     3721 atagcaaagc ctttttaggt cgccctgtca ccgggcaaac gctggatgtg cgttgtcatc
     3781 gcggcattgt taattacgac ccgaccgagc tggtgataac cgcgcgtgcc ggaacgccgc
     3841 tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gctcccctgc gagccgccgc
     3901 attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc
     3961 gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcgctg
     4021 gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac
     4081 ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg atgaaagtgt
     4141 taccgcgacc gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa gaagccatta
     4201 acgaaatcgc ccagtggcaa ctccagccgc tacccattag tggcttatgt tacttcgaca
     4261 atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcggcg cgtgaactgc
     4321 tggggggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt
     4381 tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg
     4441 atttacccgg cgagcaactg atcgactggg gcggagcgtt acgctggctg aaatcgacag
     4501 ccgaggacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta
     4561 gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc
     4621 ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt
     4681 gaggagcagg ctatgcaaac ccaattaact gaagagatgc ggcagaacgc gcgcgcgctg
     4741 gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc aacctgccca
     4801 acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta tctgattaaa
     4861 caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga tcgctgcctc
     4921 acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa tttgctggat
     4981 atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg aatgctgcgc
     5041 gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac gcaggtaggg
     5101 ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc tgaaacggtg
     5161 aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct ggaaggctgc
     5221 gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct ggatcgtctg
     5281 gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga ctatcatctt
     5341 aatgcgcagg agaaagggct ggcacgggcg cgcaataata ttgatgcctg gtggcccgcg
     5401 attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc gtttgtcaaa
     5461 gagtacgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg tcaagtcagt
     5521 gaactggcgg tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa actggcagtt
     5581 cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc gcaaaagctg
     5641 aacggcgaag tggaaaaagt gttgcttcgt cttggattta ccttaacgga cgttcccgac
     5701 agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga tctggcacgc
     5761 cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat gatcgtcacc
     5821 gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctcagt gcgtcactgg
     5881 attgaaattg tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta aagtcattct
     5941 tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc agaaaaataa
     6001 ctggtcggtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt taagtcgcat
     6061 ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta ccgccgcgct
     6121 ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta ccgcgttcgt
     6181 gactgcgccg ttactgacgt cgctggaagg cggcgtaccg gttgttgtgg atgggcaaat
     6241 tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg tcgcgaaagc
     6301 ggcagcagcg gtgttggcga aataagcgaa aacgaggaga gaaacaatga gtcaaaccat
     6361 aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg aagaagtttt
     6421 accgggaaca gggctggacg ctgcggcgtt ctggcgcaat tttgatgaga tcgttcacga
     6481 tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg cagcgcttga
     6541 tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata aatctttcct
     6601 gcgtgaactg ggctacctgg tgccgcaacc ggagtgcgtg acggtggaaa ccacgggcat
     6661 tgacagcgaa atcaccagtc aggcagggcc acagctggtg gttccggcaa tgaacgcccg
     6721 ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt tatacggcag
     6781 cgacatcatc ccgcaggaag gggcgatggt cagcggctac gatccgcagc gcggtgagca
     6841 ggttatcgcc tgggttcggc gtttcctcga tgaatctcta ccgctggaaa acggcagcta
     6901 tcaggatgtg gtggcgtttc aggtggtcga taaacaatta cgcatccagt tgaaaaatgg
     6961 taaagaaacc acgttacgta ccccggcgca gtttgtcggt taccgtggcg atgccgctgc
     7021 gccgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa ttgatgccaa
     7081 cggacggatt gggaaagacg atgcggcgca catcaacgat gttatcgtcg aagctgcaat
     7141 cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag ataaaatcct
     7201 gctgtaccgc aatctgctgg gcctgatgca ggggactctc caagagaaaa tggagaaaaa
     7261 cggtcgacaa atcgtgcgta aactgaatga cgatcgtcag tacaccgccg ccgatggctc
     7321 tgaaatttct ctgcacggac gctcgctgct gtttatccgc aacgtgggtc atttgatgac
     7381 cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc ttgatggcgt
     7441 catgactggc gcgattgccc tctatgattt aaaagtgcag aaaaactcgc gcactggcag
     7501 cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg ccaacaaact
     7561 gtttacccgc attgagacaa tgctcggtat ggcaccgaat accctgaaaa tgggcattat
     7621 ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt atcgctcagg cacgcaaccg
     7681 cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc attcggtaat
     7741 ggaagctggc ccgatgctgc gtaaaaatca gatgaaatcg acgccgtgga tcaaagccta
     7801 cgagcgtaat aacgtgcttt ccggcctgtt ctgtgggctg cgcggtaaag cgcaaatcgg
     7861 taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga agggcgacca
     7921 actgcgtgcc ggggcaaaca cagcctgggt tccgtcgcca actgccgcta cgctccatgc
     7981 gatgcactac caccaaacca acgtacagag cgtgcaagcc aacattgccc agactgagtt
     8041 caatgctgaa tttgaaccgc tgctggacga tctgctgact attccggttg ctgaaaacgc
     8101 taactggtcg gcgcaagaga tccaacaaga gctggataac aacgtgcagg ggattctggg
     8161 gtacgtggtg cgctgggtgg agcaggggat tggttgttca aaagtgccgg atattcacaa
     8221 tgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata tcgccaactg
     8281 gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga atatggcgaa
     8341 agtggttgat cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg ggaatttcgc
     8401 taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga aacagccaaa
     8461 cggctatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa gtcattaata
     8521 gcgtcaaaaa aagccccggc aagttttatt gtcggggcta tgatagtaac tgcggtagta
     8581 catttatccc ggaaataacc ggagcgatta tcagggttta acaatattaa atacccgtag
     8641 cgcattacta gcgggcctat aacaacaata tggacatctt aacgatactc tgcgcctgcg
     8701 tgtgcagaac agaaaccctt acccgatata cttgttatgc cagaggtagc aattgcggca
     8761 accgatctga ctggcatgga tgcagatgca atggtggaag atataaatta cacgataatt
     8821 acagatgttc agattaccga acgaactaaa acctatgtca gagcagataa tgctaccact
     8881 tttcggcagg gaacatcagg caccgaaatt caggctagca cagaaaccag caaccaggtt
     8941 aatctgaaat ttgaaggggc taaacctctt ctggaaatac aactgaaata aatatcctcc
     9001 ggctatgccg gaggatattt attttaattt gaagcctgat ttacacgcag ctctgcgtag
     9061 ggcagggtat catagtcagt taacgcattt ttcttatagg ggtttcctat ccagcgagtg
     9121 atttctttga aatgctggtt taatatggaa cgctctggct catagttatc ataacgtaaa
     9181 ccagccaaat ccgaccaggt atggataaaa tcagccagac tgtatttacg gttaatgtac
     9241 tcagaaaatg tatgatgtcg attaagtagc catttttcag aggcccagag aataaatgga
     9301 atattgtaca taggtcgggt tggggtgtct tcgttacgtc ccagcatttt atgtggtggc
     9361 gtatcgaata cttcctctcc atggtcagaa aaatagaaaa gaaaaccatc aggggctgtt
     9421 gctttgaaag ttttgatcaa cgttgcaaca acatggtcat taaatatatt agcattatcg
     9481 taatcgttat ataattccag ttcattatca gtaagtcctt ctggtgcatg ttccctgtcc
     9541 ccatcgaata ctccctgacc ttcaggataa cggtatttat atttgatgtg tgttcccaac
     9601 aagtgaataa taacaaattt tttgggagct ggatctgcca gtacttcact aaacggagcc
     9661 agtacgttag tatcgtactc acgggcactt tgcgttctct gctgattcat gtagtactgt
     9721 ttatctgttt gccgagagaa gactgtcagc attgtgttgc gtgcagtcat cgtttgttga
     9781 ttggtgatcc agaatgtttt gtaaccggcc tgtttcatta tattcatcag ggaaggttgt
     9841 gtcaggtaga gttcgggctg ctgttcatca gcgaaagtca atacctgctg tagtacttcg
     9901 atggtatatg gtcttgagga gacaacatta ttaaacacaa acatattttg ttccgttttg
     9961 tataattcat ctagtccggg ggttgttttt ctgggatatc catacagact cattctccca
    10021 cgctgggttg attctcctat caccagaacc agtgttcgtg gggtatctcc ggaaatatcg
    10081 ttaaagtttt tcagagcggg gagcgcatta ttttcctcca gcaaacggtt cagagaatta
    10141 agctggagac ggtactgata atatccggta ataaattgcc agggggcagc gggctccata
    10201 cgggatgcca ggcgtcccag agtattgtca atagtcctgt ttttaacaac agtatttatt
    10261 aatacagggt gcagaattaa accgtagata ataaataaag gtataatgta gcgccagtat
    10321 gatggtatat atatcgggct aagacgagtc catagcagaa tagcaatagc tgtatatatt
    10381 gtcgatataa atagtatttt taaactaaaa tactgactaa aatattcgct ggcttcactt
    10441 gtattacttt caaacatgac gaacagtaca ctctgagaga attcctgccc gtagattaga
    10501 taatagcaaa gggcagcaag agatgtgccc cataaaacca cgccgattaa tgctgctgta
    10561 acctttattc tctctgggaa aagaaaaaca gggattaacc agagagagct gaaaagtagc
    10621 gagtcacgaa gtccattggt gccactatat ccagtcagac aaatgataat ctgaagtaaa
    10681 gtggaaaaaa taccagaaat aaatgagagc ccatcccagg gctttccagc taaaaacatg
    10741 ttcatgtttt gttaatgtct tctgcatatt aataaacctg cacgggaatt tactattgta
    10801 atttttatat gagatgtatt aaaacatttc atacagattt tttgataagc acatagt
//
LOCUS       DAZKGS010000143        10800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKGS010000143 DAZKGS010000000
VERSION     DAZKGS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(400..1593)
                     /locus_tag="ACHOQY_003781"
     CDS             complement(400..1593)
                     /locus_tag="ACHOQY_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679796.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1729..3453
                     /gene="iucA"
                     /locus_tag="ACHOQY_003782"
     CDS             1729..3453
                     /gene="iucA"
                     /locus_tag="ACHOQY_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFP9679797.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3454..4401
                     /gene="iucB"
                     /locus_tag="ACHOQY_003783"
     CDS             3454..4401
                     /gene="iucB"
                     /locus_tag="ACHOQY_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFP9679798.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4401..6143
                     /gene="iucC"
                     /locus_tag="ACHOQY_003784"
     CDS             4401..6143
                     /gene="iucC"
                     /locus_tag="ACHOQY_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFP9679799.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6140..7477
                     /gene="iucD"
                     /locus_tag="ACHOQY_003785"
     CDS             6140..7477
                     /gene="iucD"
                     /locus_tag="ACHOQY_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFP9679800.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7483..9678
                     /gene="iutA"
                     /locus_tag="ACHOQY_003786"
     CDS             7483..9678
                     /gene="iutA"
                     /locus_tag="ACHOQY_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFP9679801.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9936..10151)
                     /locus_tag="ACHOQY_003787"
                     /pseudo
     CDS             complement(9936..10151)
                     /locus_tag="ACHOQY_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            10238..10663
                     /locus_tag="ACHOQY_003788"
     CDS             10238..10663
                     /locus_tag="ACHOQY_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9679802.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            10660..10734
                     /locus_tag="ACHOQY_003789"
                     /pseudo
     CDS             10660..10734
                     /locus_tag="ACHOQY_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         2569 a         2811 c         2848 g         2572 t
ORIGIN      
        1 tccagctctt tcaggtgtca acgccactct gagagagttt tatgcatccc gtattgggca
       61 ctatgatgcc ggaaatctgc gaggctcgac acctctctga ttttcccctt tatttatcac
      121 ggtgccccag atctaatgtt ggggatgcgt tcatctcatt cccaatgagt aatcgcactg
      181 ttctcattgt ctgcaggcat ccggacagag ccatgtgatg ttgcactcac aaccagcatg
      241 acttcaatgc aacttaagtg acaccggccg gttttatacc gaacataccc tgtaccatgc
      301 ggtgcatttt tgccagtgtt actgcctgac atgctttcat cagacgcagg ttctctctca
      361 tatcctctga gttactccgg taaggtgatg atcatatctt tatttgatag cacggtaatg
      421 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgccg ccataatcag
      481 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      541 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      601 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      661 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      721 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
      781 gataagcgca gctactgcgg acagacccaa cagccaggcc acctgttggc taatggcggt
      841 ggtatccgtg gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
      901 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
      961 gctcttgcgc tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1021 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1081 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1141 cagagcggcc atgcgattcc cttctcctgc tctctgcaat gcccagacct gacaggctgg
     1201 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1261 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1321 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1381 ttgctttccc caccacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1441 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1501 accgttctgc ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1561 tttttggggc gtatcttcga tcccaggtga cattttaatg tttcaactcc atgtattaat
     1621 tgtgtttatt tgtaaaatta atttatctga caataacatt tcttattgat aatgagaatc
     1681 attattgaca taattgttat tattttactg tgtgggagct gtttgactat gaccctgccc
     1741 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     1801 actacagact ggaaactgac agaatacccg ccagacgaat tgatcatccc gctggatgag
     1861 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     1921 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     1981 cggcttatta ttgataagct acggcatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2041 ttccaccagc gcgtgctgga aagccacgcc catacgcaac aggcaattga tgcccgtcat
     2101 gactggaccg ccctgcgtga aaaagcgttg aattttggcg aagctgagca ggcgctgctg
     2161 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2221 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2281 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2341 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2401 cacccgtggc agggggaata tcttttgcag caggggtggt gccaggcact tgttgctaag
     2461 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2521 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2581 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2641 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2701 gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga aagtctgttt
     2761 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agaccaacgt actggtctcc
     2821 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     2881 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga tgcatactgt
     2941 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3001 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3061 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3121 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3181 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3241 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3301 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3361 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3421 ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg aggcaaacat tattcacagc
     3481 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3541 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3601 gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3661 tgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3721 accgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     3781 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccgcagggt
     3841 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     3901 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     3961 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4021 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta ttttgaaatc
     4081 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4141 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4201 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4261 ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4321 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4381 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4441 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4501 atgaccacta ctgcattaac ctgccgggag cacaatggcg ctttatcgct gaacgtggta
     4561 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4621 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4681 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4741 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     4801 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     4861 aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg gtaaaacgtg
     4921 aacatatgat ctggcgctgc gacaacgatc tggatattca gcagttgttg actgccgcaa
     4981 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg ctggatcaca
     5041 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc accgacttca
     5101 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5161 cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg gatatcaagc
     5221 tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga tatatcgctg
     5281 ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc accctggtgc
     5341 aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat gaaggctatg
     5401 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5461 atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca ctgatggagt
     5521 gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga ctggacgctg
     5581 aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac ctgctttgcc
     5641 gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg aaagaggggg
     5701 taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg aaagaagcgt
     5761 ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt ctgagtgcgg
     5821 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     5881 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     5941 tgagtgatta catgaacaaa catccacaaa tgtcagagcg ttttgcgctt ttctcactct
     6001 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg ccggaccagg
     6061 atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg ctgtggctgg
     6121 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg ggacagggcc
     6181 atttaatctc agtattgctg cattgtcaca tcagatcgaa gaactgaact gtctcttctt
     6241 tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6301 gactgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct acagctttgt
     6361 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac tacgtacagt
     6421 atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga ataacctgta
     6481 tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt ttctggttca
     6541 gaccagtcgg ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa aacaacctta
     6601 tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg aaatgaatct
     6661 tcgtcggccg gacctgagtg gaaaacggat aactgtggtt ggtggaggac agagtggtgc
     6721 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     6781 ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     6841 acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc agttactgga
     6901 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     6961 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagccg
     7021 ctcggtaaca actctggaaa gtagtggtcc gggctggaag ttattgatgg aacatcatct
     7081 ggatcggggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7141 tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg ataagaacac
     7201 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa ataacatctt
     7261 tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca gcctgatggc
     7321 ctggagatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg atctcagtat
     7381 gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccgcagc cggaggctgc
     7441 tgctttaact cactatacaa caaatattca ggaataacga tgatgcgcaa aaagtatatg
     7501 ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc ccagcaaaac
     7561 gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc ggagatggcg
     7621 caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg cggtaaagag
     7681 ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca gagccgaacc
     7741 aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg tgtgcgcctc
     7801 aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa tatcgaccat
     7861 attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg agggttgatc
     7921 aacatcgtga ccaaaaaagg ccagccggaa accatgatgg agtttgaggc tggcacaaaa
     7981 agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt ctccggcgga
     8041 aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg ctggtttgac
     8101 ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca ctccaatcgg
     8161 ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct tcaactgata
     8221 acgcagtact ataaaagtca gggggacgac aattacgggc ttaatctcgg gaaaggcttt
     8281 tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc tgaccgcatt
     8341 cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt cctgagacag
     8401 gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggttctaccc gttcccgacg
     8461 gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac cgaccagtac
     8521 ggcatgaaac tgactctgaa cagccaactt atggacggct ggcaaatcac ctgggggctg
     8581 gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc tcaggcaagt
     8641 gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc gtcatatgac
     8701 atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat ttttaccgtc
     8761 agcggtggcg tacgctatca gtatactgag aacagggtag atgattttat cgactacacg
     8821 cagcaacaga agattgctgc cgggaaggcg atatctgccg acgccattcc tggtggttcg
     8881 gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac cgaacgtcag
     8941 caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa atattatggt
     9001 cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag cgtgaacgtc
     9061 agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg gcgctttacc
     9121 ggtgacaacc tgcggactca aatcgcagca tattactcgc tttccaataa gagcgtggaa
     9181 aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta cggcgtggaa
     9241 ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa cttcaatgtg
     9301 ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa ggaatcaagt
     9361 ccatcgaaag cgacagctta cattagctgg gcgccggaac cgtggagtct gcgtgtgcag
     9421 agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg ttacactacc
     9481 gtcgatttta tcagtagttg gctgcttccg gtgggaacac tcagcttcag cgttgagaac
     9541 ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta ctacagcccg
     9601 ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt tggtctgaac
     9661 tactcagtgc tgttctgacg gggattcttt tgcaacaagg gtatgctgta aacaacatgg
     9721 tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa gatattccgt
     9781 ctccggaatg gactgttcac cactgaataa caatatgtct gttttatgga tctttgactg
     9841 aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac aagtgaaccg
     9901 ccccgggttt cctggagagt gttttatctg tgaactcagg ctgccagatc atcgtttccg
     9961 atggaagcat aataagcttt ttctgcttct gccggaggag tatggcccag cctttccagc
    10021 aatcgtcgat tgttatacca gtccacccac gtgagtgtgg ccagttccac ttctgcacgg
    10081 tttttccagc tcttacggtg tattacctcc gctttgtaaa gaccattgat gctctccgcc
    10141 atcgcgttgt cagtaagcgt aaactgaccg ccgtatgtag ccatcagacg aaaattggta
    10201 acttagacgc ccatctgata tagacggaca tctaagtatg gaattacagg actggcgaaa
    10261 agaacctcgt aaaaactatt cgaatgaatt caaacttcgt atggtggaac tggcatcaca
    10321 accaggagcc tctgttgccc gtattgcccg ggaacacgat atcaatgata acctcctgtt
    10381 caaatggctc aggctctggc agaacgaagg gcgtatatca cggcgtcttc aggtaacgac
    10441 ctcttctgac actggcgttg aattattacc tgtagaaata acgccggatg agcagaaaga
    10501 accggtggcg gccattgcgc cgtctttatc cacttccact cagaccagtg tcagtgccgg
    10561 ctcctgcaag gtggagttcc gtcacggtaa catgacgctg gaaaatccat cgccagagct
    10621 gctcacgctg ttgatccgtg aactgaccgg gaggggacga tgatctcact cccgtcaggt
    10681 acccgcatct ggctcgttgc cgacgttacc gatatgcgta aatccttcaa cggatacgac
    10741 atatgctgaa ggaggagcag taatggatta agtgataaca aatacccaga aatagaggtt
//
LOCUS       DAZKGS010000144        10728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKGS010000144 DAZKGS010000000
VERSION     DAZKGS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..400)
                     /locus_tag="ACHOQY_003790"
     CDS             complement(47..400)
                     /locus_tag="ACHOQY_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9679803.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHOQY_003791"
     CDS             complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHOQY_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9679804.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2228..2521)
                     /locus_tag="ACHOQY_003792"
     CDS             complement(2228..2521)
                     /locus_tag="ACHOQY_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9679805.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHOQY_003793"
     CDS             2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHOQY_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9679806.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4069..4545)
                     /locus_tag="ACHOQY_003794"
     CDS             complement(4069..4545)
                     /locus_tag="ACHOQY_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9679807.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4882..6318
                     /gene="aspA"
                     /locus_tag="ACHOQY_003795"
     CDS             4882..6318
                     /gene="aspA"
                     /locus_tag="ACHOQY_003795"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9679808.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHOQY_003796"
     CDS             6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHOQY_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9679809.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7853..8191
                     /gene="cutA"
                     /locus_tag="ACHOQY_003797"
     CDS             7853..8191
                     /gene="cutA"
                     /locus_tag="ACHOQY_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9679810.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHOQY_003798"
     CDS             8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHOQY_003798"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9679811.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9901..10476
                     /locus_tag="ACHOQY_003799"
     CDS             9901..10476
                     /locus_tag="ACHOQY_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9679812.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10583..10658
                     /locus_tag="ACHOQY_003800"
     tRNA            10583..10658
                     /locus_tag="ACHOQY_003800"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10616..10618,aa:Phe,seq:gaa)
BASE COUNT         2496 a         2819 c         2680 g         2733 t
ORIGIN      
        1 agcgcatcag gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact
       61 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
      121 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
      181 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
      241 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
      301 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
      361 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
      421 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
      481 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
      541 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
      601 atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga
      661 agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
      721 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
      781 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
      841 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
      901 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
      961 ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat
     1021 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
     1081 gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     1141 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
     1201 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     1261 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     1321 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     1381 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat
     1441 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     1501 agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     1561 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     1621 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     1681 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     1741 accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc
     1801 aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     1861 agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt
     1921 accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat
     1981 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     2041 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
     2101 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     2161 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     2221 gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     2281 cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     2341 ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     2401 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     2461 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     2521 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     2581 tttcccggct ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag
     2641 aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
     2701 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
     2761 gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg
     2821 gggctggccc aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc
     2881 gttcctgcgt tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg
     2941 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
     3001 gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc
     3061 ggctggttgt ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc
     3121 gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
     3181 acgctggcgc tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa
     3241 accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc
     3301 aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct
     3361 gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg
     3421 gaatttaaaa atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca
     3481 ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa
     3541 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
     3601 tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag
     3661 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc
     3721 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg
     3781 agcacgttgg tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat
     3841 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga
     3901 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc
     3961 ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa
     4021 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
     4081 atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa
     4141 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa
     4201 atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc
     4261 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac
     4321 acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac
     4381 gaaattctta aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac
     4441 cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
     4501 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
     4561 tagcagccgg taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
     4621 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
     4681 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
     4741 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
     4801 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
     4861 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
     4921 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
     4981 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
     5041 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
     5101 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
     5161 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
     5221 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
     5281 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
     5341 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
     5401 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
     5461 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
     5521 gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
     5581 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
     5641 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
     5701 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     5761 atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac
     5821 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     5881 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     5941 gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc
     6001 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     6061 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     6121 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     6181 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     6241 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     6301 gatgaaagcg aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg
     6361 tgcctttttt cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa
     6421 acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg
     6481 ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt
     6541 gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg
     6601 gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag
     6661 acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc
     6721 tatttcctga ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc
     6781 gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta
     6841 tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc
     6901 gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc
     6961 ctgctggcgg ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct
     7021 gacgatccga tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag
     7081 cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt
     7141 ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg
     7201 atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt
     7261 atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc
     7321 gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc
     7381 gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc
     7441 atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg
     7501 ccgatggccc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg
     7561 tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac
     7621 acgggtacaa cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact
     7681 ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga
     7741 ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc
     7801 cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga
     7861 tgaagaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac
     7921 agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat
     7981 ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca
     8041 gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca
     8101 tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta
     8161 cctctcatgg ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt
     8221 gccggattat tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct
     8281 tttgattttc agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac
     8341 tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag
     8401 ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat
     8461 cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc
     8521 taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta
     8581 agcgaagtgg tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag
     8641 cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc
     8701 tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa
     8761 cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca
     8821 ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg
     8881 ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca
     8941 atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg
     9001 agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga
     9061 ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa
     9121 agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg
     9181 ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg
     9241 gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag
     9301 cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt
     9361 ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata
     9421 atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg
     9481 acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag
     9541 ttaaatcagg cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac
     9601 tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa
     9661 gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg
     9721 gcactgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa
     9781 ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca
     9841 catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc
     9901 gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc
     9961 aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc
    10021 ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata
    10081 gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg
    10141 gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt
    10201 atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag
    10261 caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat
    10321 ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg
    10381 gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc
    10441 ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt
    10501 tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga
    10561 ttacggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa
    10621 tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca
    10681 cggaggtccg tttttcgttt cagaacacca tgatttaagt gttcttcc
//
LOCUS       DAZKGS010000145        10705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKGS010000145 DAZKGS010000000
VERSION     DAZKGS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..673
                     /locus_tag="ACHOQY_003801"
     CDS             <1..673
                     /locus_tag="ACHOQY_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP9679813.1"
                     /translation="VSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDA
                     CVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGS
                     SVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(755..1093)
                     /gene="rcnB"
                     /locus_tag="ACHOQY_003802"
     CDS             complement(755..1093)
                     /gene="rcnB"
                     /locus_tag="ACHOQY_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9679814.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1312..2136)
                     /gene="rcnA"
                     /locus_tag="ACHOQY_003803"
     CDS             complement(1312..2136)
                     /gene="rcnA"
                     /locus_tag="ACHOQY_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9679815.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            2257..2529
                     /gene="rcnR"
                     /locus_tag="ACHOQY_003804"
     CDS             2257..2529
                     /gene="rcnR"
                     /locus_tag="ACHOQY_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9679816.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      2621..2717
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2752..3540
                     /gene="thiM"
                     /locus_tag="ACHOQY_003805"
     CDS             2752..3540
                     /gene="thiM"
                     /locus_tag="ACHOQY_003805"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9679817.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            3537..4337
                     /gene="thiD"
                     /locus_tag="ACHOQY_003806"
     CDS             3537..4337
                     /gene="thiD"
                     /locus_tag="ACHOQY_003806"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9679818.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            4402..5220
                     /locus_tag="ACHOQY_003807"
     CDS             4402..5220
                     /locus_tag="ACHOQY_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9679819.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            5272..6018
                     /locus_tag="ACHOQY_003808"
     CDS             5272..6018
                     /locus_tag="ACHOQY_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9679820.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5992..6957)
                     /locus_tag="ACHOQY_003809"
     CDS             complement(5992..6957)
                     /locus_tag="ACHOQY_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9679821.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(6954..7958)
                     /locus_tag="ACHOQY_003810"
     CDS             complement(6954..7958)
                     /locus_tag="ACHOQY_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9679822.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(7955..9232)
                     /locus_tag="ACHOQY_003811"
     CDS             complement(7955..9232)
                     /locus_tag="ACHOQY_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9679823.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            9489..10541
                     /gene="fbaB"
                     /locus_tag="ACHOQY_003812"
     CDS             9489..10541
                     /gene="fbaB"
                     /locus_tag="ACHOQY_003812"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9679824.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         2778 a         2720 c         2729 g         2478 t
ORIGIN      
        1 tgtttctgcg gggggcggcg gttcagtgat gattaatgga gttgcgacat tatccagtgc
       61 ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat taggtggcaa
      121 gtcatgggat gcatgtgtaa atagctacag aaatgcattg gcacaaaatg caggtgtcta
      181 ttcctttaat ctgacattgt catacaaccc gataaccaca acctgcaaac cggacgattt
      241 attaattact ttagacagta ttcccgtttc acaattacca gccacaggta acaaagcaac
      301 aataaatagt aaacaagggg atattattct gcgttgtaaa aatttattag gtcaacaaaa
      361 tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa ccaacagcaa
      421 cacaatactg aaaggtgcgg aagataatgg cgtaggattt attcttgaaa gtaatggttc
      481 gtcagtcaca cttttaaata tcactaacag cagtaaacga tacacaaatt taaaagaaat
      541 tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataactg ccagttacta
      601 cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat taatcaacgt
      661 gaaatacgac taataacaag caagacgagc aagtggctaa taataaaaaa taacaaggtg
      721 tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa atctcaccgt
      781 cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg ccacccatat
      841 aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac tgcttaatgt
      901 tgtactcatc ggtgcggtac atctctggga cggtatcgcc tggtttgaaa tgcttaaagt
      961 cagcaatgaa actgcttaac tcatacttag aaattcccga ggtattggtc gaacccgcgg
     1021 tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cgcacccagc aacatcttat
     1081 ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca tatctttcct
     1141 gccatacaat ataactgaca agcgatattt tgttagtgat cccatgattt cgtaaaaagt
     1201 gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg gaaatagctg
     1261 atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga gttatcgcat
     1321 tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca acagactgga
     1381 aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg cgacctgctg
     1441 aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc caatgctgaa
     1501 actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata tcaacagcac
     1561 ggtaattgct gccgggcagg ggataaggcc accggttaag ccaaataaca aaatttgcca
     1621 gttggtgacc tctctaccat caaagcgtcg tttaatgtca ttagcatggg ctcgtgcatg
     1681 agcatcctga tactcgccat gttcgtgatg gtgatgatgt ccatggtcgt ggtgatcttc
     1741 gtgatcgtga tgatgatgct catgatcatg ctcgtgcata ttctccagcc agttgcgctc
     1801 gccgtgccag gtacgccaga acatccagaa cgcggtgcta ataatgatca ctgcggaaat
     1861 caactggagc cacggttctg ctgattgagc agtaaagcgc ttgctgacca ccatcccgcc
     1921 aaaggcaatc aaccagacca ctgcggtatg cgaaatagtt gctgccagtc cgagcatcac
     1981 cgcctgttta atggtgcctt tgatggcgat gataaacgcc gccatcatcg tttttgagtg
     2041 ccctggttcc aggccatgaa gcgcaccaag taagatggcg ctggggatga agaaccaggc
     2101 gtttccttgc tgaagaagag ttgtaaattc ggtcatgata ataattctta gtattaattc
     2161 ggcaatctga ttctactccc ccccagtacc tgatactacc ccccagtaga ttaatagtgc
     2221 tatgattttt catattcttg ttaaccaggt gttgccatgt ctcatacaat ccgtgataaa
     2281 cagaaactga aagcgcgtgc cagtaagatt cagggccagg tcgtggcgct caagaaaatg
     2341 ctcgacgaac cgcacgaatg cgctgcagtt ttacaacaga ttgctgctat ccgtggcgcg
     2401 gtaaacggtc tgatgcggga agtgattaaa ggtcatctga cggaacacat cgttcaccag
     2461 ggggatgagc taaaacgtga agaagatctg gatgtcgttc tgaaggtgct ggattcatat
     2521 atcaaataat ttattaacgc gattgtaaaa ctgccgtttt tcctcgttta caacgcgtgc
     2581 gctggacatt accatcctcc tctgcgattt atcatcgcaa ccaaacgact cggggtgccc
     2641 ttctgcgtga aggctgagaa atacccgtat cacctgatct ggataatgcc agcgtaggga
     2701 agtcacggac caccaggtca ttgcttcttc acgttatggc aggagcaaac tatgcaagtc
     2761 gacctgctga gttcagcgca atctgcgcac gcgttacacc tttttcacca acattcccct
     2821 cttgtgcact gcatgaccaa cgatgtggtg caaaccttta ctgccaatac cttgctggcg
     2881 ctcggtgcat cgccagcgat ggttatcgaa accgaagagg ccagtcagtt tgcggctatt
     2941 gccagtgcgt tgctgattaa cgttggcacg ctgacgcagc tacgcgcaca gtcgatgtgt
     3001 gccgccgttg agcaagcaaa aagctctcaa acaccctgga cgcttgatcc agtagcggtt
     3061 ggtgcactcg attatcgccg ccgtttttgt ctggaacttc tgtctcataa gccaaccgcc
     3121 atacgtggta atgcttcgga aatcatggca ttagctggca ttgctaatgg tggacgggga
     3181 gtggatacca ctgacgccgc agctaacgcg atacccgctg cacaaacact ggcacgggaa
     3241 actggcgcaa tcgtcgtggt cactggcgag atggattatg ttaccgatgg ccatcgcgcc
     3301 gttggcattc acggtggcga tccgttaatg accaaagtgg taggaactgg ctgtgcatta
     3361 tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata cgctggaaaa tgtcgcatct
     3421 gcctgtcact ggatgaaaca agccggagag cgcgcagtcg ccagaagcga ggggccaggc
     3481 agttttgttc cacatttcct tgatgcgctc tggcaattga cgcaggaggt gcaggcatga
     3541 aacgaattaa cgctctgacg attgccggta ctgatcccag tggtggtgcg gggattcagg
     3601 ccgatcttaa aaccttctcg gcacttggcg cttatggttg ctcagttatt actgcactgg
     3661 tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat tgagcctgat tttgtcgccg
     3721 cccagctcga ttcggtgttc agcgatgtgc gaatcgacac cactaaaatc ggtatgttgg
     3781 cggaaaccga tattgttgaa gcggtggcag aacggttgca acgttatcag atccaaaacg
     3841 tggtactcga caccgttatg ctggcaaaaa gcggcgaccc gctgctttca ccttcggcgg
     3901 ttgctacgct gcgcagtcga ttattgccac aggtttcatt aataacgcca aacttgcccg
     3961 aagctgccgc cttgctcgac gcgccacacg cgcgcaccga acaggaaatg ctggaacaag
     4021 ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat gaaaggtggt catctggatg
     4081 atgagcaaag cccggactgg ctgtttaccc gcgagggtga acaacggttt accgcaccgc
     4141 gcattatgac caaaaacacc cacggcactg gttgtacgct ctctgcggcg ttggctgcac
     4201 tacgcccgcg ccatacaaac tgggctgaca ccgtacagga ggcaaaaagc tggctttcat
     4261 cagcattagc ccaggccgac acgctggaag ttggtcacgg tattggtccg gttcaccact
     4321 tccacgcctg gtggtgagca ccgtactata gctgctttgg ttattgtcag cctttggcac
     4381 tgacattcag ggatgaaacc aatgcagcta agaattacca gtcgaaaaaa actcacctct
     4441 ctattatgcg ctcttgggct aatttccatc gttgcaattt atccgcgtca gacggtgaat
     4501 tttttctatt cgacagcagt tcagattaca gactacatcc atttctacgg ttatcgcccg
     4561 gttaaatctt tcgctattcg cattcctgcc agttacacca ttcacggaat agatgtttct
     4621 cgctggcagg agcggatcga ctggcagcgg gtggcaaaaa tgcgcgacaa cggtatccgc
     4681 ttacagtttg cttttattaa ggcgacggaa ggcgaaaagc tggtggaccc gtatttttcg
     4741 cgtaactggc aactaagccg cgaaaatggc ctgctgcgcg gggcgtatca ttatttctcc
     4801 ccgtcggtag cagcgccggt tcaggcacga ttatttctgc aaacggtgga tttctcacaa
     4861 ggcgatttcc ctgcggtact ggacgtagaa gaacggggaa aattatcggc aaaagaatta
     4921 cgcaaacgag taagtcagtg gctaaaaatg gtcgaaaaaa gtacgggaaa aaagccgatt
     4981 atttactcag gagccgtttt ttatcacacc aatctggcgg gctatttcaa tgaatatccg
     5041 tggtgggtgg ctcactatta tcaacgtcgt ccggacaatg acggcatggc ctggcgcttc
     5101 tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg gtccggtgga ttttaatgta
     5161 tttcatggca cggtggagga gttgcaggca ttcgttgatg ggattaaaga aacgccttaa
     5221 ataagaagca gtgatatact cccaccagac tccccggcag gaaaaagaca aatggaacaa
     5281 gcgcataccc agctgattgc ccaactgaac gagcgtattt tagcggcgga taacacgccg
     5341 ctttatatta agtttgccga aacggtaaaa aatgccgtgc gcagcggggt gctggagcat
     5401 ggcaatattt tgcccggtga gcgtgattta agtcagttaa ccggcgtttc gcgcattacg
     5461 gtgcgcaagg cgatgcaggc gctggaagaa gagggtgtgg tgacgcgttc gcgcggttac
     5521 ggcacgcaga tcaacaacat cttcgaatac tcgttgaaag aagcgcgagg tttttctcag
     5581 caggtggtat tgcgcgggaa aaagcccgat acgctatggg ttaacaagcg tgtcgtgaaa
     5641 tgtcccgaag aagtcgcgca gcagctggcg gtcgaagcag gaagtgatgt ctttttgctt
     5701 aagcgtattc gctatgtcga tgaagaagcg gtatcgattg aggaatcgtg ggttccggcg
     5761 catttaattc atgatgttga tgccatcggg atttcacttt atgactattt ccgcagccag
     5821 cacatttacc cacaacgtac gcgttcccgc gttagcgccc ggatgccgga tgccgagttt
     5881 cagtcacata ttcagttaga tagcaaaata ccggtgctgg tgatcaagca agtggcgctt
     5941 gaccaacagc aacggcctat tgagtacagc attagccact gtcgcagcga tctatacgtt
     6001 tttgtgtgcg aggagtagtt cctcgcgcgt tggcgcacaa tcaccgcccc gatgcccgac
     6061 aacccacgac gccactgcat tgcccagtaa tacggcatcc gccagtggca gaccagaggc
     6121 cagcccggca agtacgccac ctgcatgact gtcgcccgcc ccaatggtgt ctacaacttg
     6181 cgttggaaat gccggaatgc agccagaagc gtcgttgctg aaataccatg caccctcttt
     6241 atcgagacga acgatcaacg gcgcggcaaa tttctcctgc cattgcttgc caagtgttgt
     6301 tatctctgcg gataaagcaa aacgttcggc ggcaatctca gcctcttgac gattgagtga
     6361 tactaaaggt cgacaggcca tgatccgcgc cagtaatgcg tccgggatat cgccgatacg
     6421 tgggccaaaa tcgataaacg gcgtcacgtc ttgcagcttt tccagccatt ccactaacaa
     6481 ttcgccgcag ggcgaggcca gttgataacc ggaaaaatag agcaggctgc caggcgcaac
     6541 ggttaatcgc gccagccact ggcgattcca ctgattttcc acaccgctga atgacataaa
     6601 agtgcgttcg ccatccggct caaccagcgc cagacaccag ccgttatcgc cttcggcgtt
     6661 atcgatcagg ctgattaacc cctctttcgc catccggttg cgaataatct ccgcccacac
     6721 gccctgaccg agcggcaagg cattacccgc ttcgatgccg aggcgcttta acgccacggc
     6781 aatattcagt gcgcagccgc caacgttaac gctctgctgc ttcagttcga tatcgcaccc
     6841 acgccagggg agggcataag cgtcggcgat cacatcaatg accgccgcgc cgacgaccat
     6901 caccggctga cgcgaggtta attcaggcag cagcgtgtgt aatcgagcgc cgctcatacc
     6961 gcctcccgtt gttgacgata tttcgccagc gctgtggcat agcggttgaa atcaagctga
     7021 tttaccgcat ccagttccgc ctttaatgca ggatcgatag cgttaacgcc atgcaacgcg
     7081 ccgcaaatcg ccgtcgccat agcgccgatg gtgtctgtgt cgccgccaag gttagcgcac
     7141 aggacggcgc agcgattcgg gtcggtttgt gccagttcaa ccagcgcaat ggcgcacgga
     7201 acggactcaa tagtgctggt acctgcgcca acgacctggt aaagctgttc gctggcggat
     7261 tcggtgccgt cggcattgcg cacaattttc agcgcaatct ccagacgtgc tgccagtgag
     7321 gcgctgaagg tagtgatgcg tttttgctgt gcatgtcgcg caattgaagg cagggaatca
     7381 acaatcgctg accagctttc tccgtcaatg gcacgagaaa tcgcccatgc gatgactacc
     7441 gcacctgcaa ctgccagatc ggatttatgg gtcgggctcg acgccagcgc cacatcatca
     7501 ataaaggaat caacatcacg cgccggaagc aaacaaccta atggcgagac gcgcatcgct
     7561 gcgccgttgg tcacgccgtt gttttccagt tcagcaacgg gtttaccgtc gcgaatggcg
     7621 ttaagcgcaa tcttcgaggt cggacctagt acgtttttgt taaaggcgtc gaaacgcagc
     7681 gcccagtcga gaatattacg cccaatcaga tccggatcga tcttgccttc acgttccagt
     7741 aacgcatccg ccagacacag cgccatcgag gtatcgtcgg tgaattcggc gcggttaaaa
     7801 taacaggccg cgttattctc ctttggtcca ggcagaaaac ggtcaatcca gccaaagtgt
     7861 gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc ccctaacgcc
     7921 tgcccataaa gagcaccgag aatacgttct gttttcattt aacttcccct tgtgtcaacg
     7981 caatatcgcg atcatcgacc ttgatagccg taatttcgtt gtcggattcg cggaaaaaaa
     8041 tcatgaacag cacggcgata atggcaatca tcaccgcgcc gaaagtccac atcccggacc
     8101 agttgaaagt cagcccgttt accggttccg gataagcgaa catcttttcc atcatcacac
     8161 cgccaagacg atagccgagc aaactgccga agccctggca gcagagcgtg atcagcccct
     8221 gcgcggcggt acgcatatgc acgggggctt ttttatcgac atagatgtaa gcggtaacgt
     8281 agtaaaaatc gtaacttacg ccgtgaagca aaataccgag gaacagtaac gcgtaggtga
     8341 aatattcatc cgcactaccg taaataaaga agccatagcg gatcgcagcg gtgaccagac
     8401 caagcaataa tacctttttg ataccaaagc gtttagtgaa aaacggcaat gccagcataa
     8461 agaagatttc agagaactgg ccgagcgtca tccagccggt ggcgtttttc atgccaactt
     8521 cggtcagata accgttggca aagatgtaat agaacgccag tggcatcgca aacaggaatg
     8581 aacagaagaa aaagacgagg aagtttttat cgcgcagcag gatcagcgca tccaggccga
     8641 gcatgacttt aatgtccatt ttgccggtgc tttttggcgg cgtgtcgggc aggaaaaacg
     8701 caaacacacc gagcagagca gaacttccgg cggtaatcag cagcgggatg ttagtcggtg
     8761 agatatcggc ataccccagc atttgcggca agaaaccaca tgccagacca gaggcaatcc
     8821 agccgatagt gcccatcaca cgaatgcgcg ggaaatcacg ctccacatcc ggcacgttgg
     8881 caaaagcgat actgttagtc agcgcaatgg tcggcatata ggttagcgag taggccagca
     8941 gtaacgggaa gaacccggca aaagtggtct gttgcgcagc gaaatacatc agcagcgcgc
     9001 ctgcgaacat caataccgcc agcacttttt gcgccgagaa aaagcggtca gtgatggagc
     9061 caaccagaat tggcgacagg atcgccgcaa tggctgtgca ggcatacgac cagccaattt
     9121 ctccggcact aaaaccgctt ttacttaacc acaaccacaa tggcacaaac cacgcgcccc
     9181 agataaacca ttcaacaaac atcatgaacg acagctttgc tgttgttttc atcttttaat
     9241 tccttcatga caggtaatgt aaggtacgcg atgacaatac cacttaataa taccttttaa
     9301 atacctttgc ggtgaaaact tgaccgcgct aacatttttt ctgatgaatc gagccaacag
     9361 aaaacgctga aaaaacatcc aaaagatgga aaaactcgcc cgtaacgacc aggattatga
     9421 ttgcccgcaa aaggcgggcc aggacaatcc atagccgata tccaatcgga atttacggga
     9481 gcatagtaat gacagatatt gcgcagttgc ttggcaaaga cgccgacaac cttttacagc
     9541 accgttgtat gacaattcct tctgaccagc tttatctccc cggacatgac tacgtagacc
     9601 gcgtaatgat tgacaataat cgcccgccag cggtgttacg taatatgcag acgttgtaca
     9661 acaccgggcg tctggctggc acaggatatc tttctattct gccggttgac cagggcgttg
     9721 agcactctgc cggagcttca tttgctgcta acccgctcta ctttgacccg aaaaacattg
     9781 ttgaactggc gatcgaagcg ggctgtaact gtgtggcgtc aacttacggc gtgctggcgt
     9841 cggtatcgcg gcgttatgcg catcgcattc cattcctcgt caaacttaat cacaacgaga
     9901 cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag caggcgttca
     9961 acatgggcgc ggttgcggtt ggtgcgacta tctattttgg ctcggaagag tcacgtcgcc
    10021 agattgaaga aatttctgcg gcttttgaac gtgcgcacga gctgggtatg gtgacagtgc
    10081 tgtgggccta tttgcgtaac tccgccttta agaaagatgg cgttgattac catgtttccg
    10141 ccgacctgac cggtcaggca aaccatctgg cggcaaccat cggtgcagat atcgtcaaac
    10201 aaaaaatggc ggaaaataac ggcggctata aagcaattaa ttacggttac accgacgatc
    10261 gtgtttacag caaattgacc agcgaaaacc cgattgatct ggtgcgttat cagttagcta
    10321 actgctatat gggtcgggct gggttgataa actccggcgg tgctgcgggc ggtgaaactg
    10381 acctcagcga tgcagtgcgt actgcggtta tcaacaaacg cgctggcgga atggggctga
    10441 ttcttggacg taaggcgttc aagaaatcga tggctgacgg cgtgaaactg attaatgccg
    10501 tgcaggacgt ttatctcgat agcaaaatta ctattgcctg atacgcctca tacgaacagc
    10561 accaccccgt aggacggata aggcgtttac actgcatccg gcaacctatg cctgatgcga
    10621 cgcggaagcg tcttatcatg cctacatagc actgccacgt atgtttacac cgcatccggc
    10681 ataaaaacac gcgcactttg ctacg
//
LOCUS       DAZKGS010000146        10440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKGS010000146 DAZKGS010000000
VERSION     DAZKGS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..613
                     /gene="ssuE"
                     /locus_tag="ACHOQY_003813"
     CDS             38..613
                     /gene="ssuE"
                     /locus_tag="ACHOQY_003813"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9679825.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            606..1565
                     /gene="ssuA"
                     /locus_tag="ACHOQY_003814"
     CDS             606..1565
                     /gene="ssuA"
                     /locus_tag="ACHOQY_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9679826.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1562..2707
                     /gene="ssuD"
                     /locus_tag="ACHOQY_003815"
     CDS             1562..2707
                     /gene="ssuD"
                     /locus_tag="ACHOQY_003815"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9679827.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2718..3509
                     /gene="ssuC"
                     /locus_tag="ACHOQY_003816"
     CDS             2718..3509
                     /gene="ssuC"
                     /locus_tag="ACHOQY_003816"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9679828.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3506..4273
                     /gene="ssuB"
                     /locus_tag="ACHOQY_003817"
     CDS             3506..4273
                     /gene="ssuB"
                     /locus_tag="ACHOQY_003817"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9679829.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4480..7092)
                     /gene="pepN"
                     /locus_tag="ACHOQY_003818"
     CDS             complement(4480..7092)
                     /gene="pepN"
                     /locus_tag="ACHOQY_003818"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9679830.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7358..8560
                     /gene="pncB"
                     /locus_tag="ACHOQY_003819"
     CDS             7358..8560
                     /gene="pncB"
                     /locus_tag="ACHOQY_003819"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9679831.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAVAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8729..10129
                     /gene="asnS"
                     /locus_tag="ACHOQY_003820"
     CDS             8729..10129
                     /gene="asnS"
                     /locus_tag="ACHOQY_003820"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9679832.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2363 a         2690 c         2804 g         2583 t
ORIGIN      
        1 ttaacaaaaa aagaacaatt ggttataagg agagagtatg cgtgtcatca ccctggcggg
       61 tagtcctcgc tttccttctc gctccagctc cttgctggaa tatgcgcggg aaaaactaaa
      121 ttgcctggat gtagaggttt atcactggaa tctgcaaaac ttcgccccgg aagatctgct
      181 ttacgctcgt ttcgatagtc cggcactcaa gaccttcacc gaacagctgc aacaggctga
      241 tggactgata gtcgctacac ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct
      301 gctcgacctg ctgccagagc gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg
      361 cggtacggtg gctcatctgc tggcggtcga ttatgccctt aagccagttt taagtgcgat
      421 gaaagcccag gagatcctgc acggcgtgtt tgccgatgac tcacaagtta ttgattacca
      481 tcacaaaccc cagttcacgc caaatctgca aacccgtctt gataccgcgc tagaaacttt
      541 ctggcaggca ttgcaccgcc gcgatgttca ggttcctgac cttctgtctc tgcgaggtaa
      601 tgtccatgcg taacatcatt aaactggcgc tggcgggatt gcttagcgtc tctacttttg
      661 cggttgctgc agaatcctcg cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg
      721 gtatggtatt ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa tcaaaaatct
      781 cctgggtgga gttccccgcg ggtccgcaaa tgttggaagc gttaaacgtt ggcagtattg
      841 atctcggcag taccggggat attccgccaa tctttgccca ggctgccggg gctgatttgg
      901 tatatgtggg cgtcgagcca ccgaagccca aagccgaagt gattctggtg gcagaaaaca
      961 gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt tgcctttcag aaaggttcca
     1021 gttcacacaa ccttttactg cgtgcactgc gtcaggccgg acttaagttt accgatatcc
     1081 aacccactta cctgacgccc gctgatgccc gcgccgcgtt ccagcaaggt aacgttgacg
     1141 cctgggctat ctgggatccc tactactctg ctgcattatt acagggcgcc gtgcgggtgc
     1201 tgaaagacgg caccgatctc aatcaaactg gatcgtttta tctggcagct cgtccctatg
     1261 cagaaaaaaa cggcgctttt attcagggcg tactcgcaac ctttagtgag gccgatgcgt
     1321 taacccgcag ccagcgcgag caaagcatcg ctttactggc aaaaacgatg ggcttaccgg
     1381 caccggtgat tgcctcttac ttagatcatc gccctcctac caccatcaaa ccggttaacg
     1441 ccaaggttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaat cgtctggtgc
     1501 cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac tcaactggaa ggaaaacaat
     1561 tatgagtctg aatatgttct ggtttttacc gacccatggt gacgggcatt atcttggaac
     1621 ggaagaaggt tcacgcccgg ttgatcacgg ctatctgcaa caaattgcgc aagcggcgga
     1681 tcgtctgggc tataccggtg tgctgatccc aacggggcga tcgtgtgaag atgcgtggct
     1741 ggtggcggcg tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg ccctgcgccc
     1801 cagcgtaacc tcacctaccg ttgccgctcg ccaggccgcc acgcttgacc gtctctcaaa
     1861 tggtcgtgcg ttgtttaatc tggtcacagg cagcgatcca caagagctgg caggcgacgg
     1921 cgtgttcctt gatcatagcg agcgctacga agcctcggcg gaatttaccc aggtctggcg
     1981 gcgcttattg ctgggggaaa ccgtcaattt caacggtaaa catattcatg tgcgcggagc
     2041 aaaactgctc ttcccgccga ttcaacagcc ttatcctccg ctttactttg gcggatcgtc
     2101 agacgttgcc caggagctgg cggcagaaca agttgatctc tacctcacct ggggcgaacc
     2161 gccagaacta gtaaaagaga agatcgaaca agtgcgggcg aaagctgccg cgcatggacg
     2221 caaaattcgt ttcggtattc gtctgcatgt gattgttcgt gaaactaacg acgaagcgtg
     2281 gcaggccgcc gagcggttaa tctcgcatct tgatgatgaa actatcgcca aagcacaggc
     2341 cgcattcgcc cggacggatt ccgtaggaca acagcgaatg gcggcgttac ataacggcaa
     2401 gcgcgacaat ctggagatca gccccaattt atgggcgggc gttggcttag tgcgcggcgg
     2461 tgccggaacg gcgctggtgg gcgatggtcc tacggtcgct gcgcgaatca acgaatacgc
     2521 cgcgcttggc atcgacagct ttgtgctttc gggctatccg catctggaag aagcgtatcg
     2581 ggtcggcgag ttgctgttcc cacatctgga tgtcgccatt ccggaaattc cccagccgca
     2641 gccgctgaat ccgcaaggcg aagcggtggc gaatgatttt atcccccgta aagtcgcgca
     2701 aagctaagga agaaataatg gcaacgccag tgaagaagtg gttattgcgc gttgccccct
     2761 ggtttttacc ggtgggcatc gtggcggtgt ggcaactggc ctcctcagtt ggctggctgt
     2821 cgacgcgtat tttgccctca ccggaagggg tggtgacggc gttctggacg ctctccgcca
     2881 gcggcgaact gtggcagcat ctggcgatca gttcctggcg ggcgctgatt ggcttttcaa
     2941 ttggcggatc gctggggttg attttgggac tgattagcgg gctatctcgc tggggagaac
     3001 ggctgctgga tacctcaatt cagatgttgc gcaacgtacc gcatctggcg ctgatcccgc
     3061 tggtgatttt gtggtttggc attgatgaat ccgcaaaaat ctttctggtg gcgctcggca
     3121 cgctattccc catttatatc aacacctggc atggcatccg taatatcgat cgcggtctgg
     3181 tggaaatggc gcgtagctat ggattatccg gcataccgct gtttatccat gtgatcctgc
     3241 ctggtgccct gccctcaatt atggtcggcg tgcgttttgc gttgggcctg atgtggctga
     3301 cgctgattgt tgccgaaacc atttctgcca attcaggcat tggttatctg gcgatgaatg
     3361 cgcgggagtt tttgcaaacg gacgtggtgg tagtcgctat tattctttac gccctgctcg
     3421 gcaaacttgc tgacgtcagc gcgcagctgt tagagcgcct ctggttgcgc tggaacccgg
     3481 cttatcattt gaaggaggcc acggtatgaa tactgctcgt ctgaaccagg gcacaccatt
     3541 gttgctcaat gcagtaagca aacattacgc ggaaaatatc gtcctgaacc aactggattt
     3601 acatattccg gcaggtcagt ttgtggcggt ggtgggccgc agcggtggtg gcaaaagtac
     3661 cctgctgcgc ctgctggcag gtctggaaac gccaaccgca ggcgatgtgt tagcgggcac
     3721 cacaccgctg gctgagattc aagaagacac gcgaatgatg tttcaggatg cgcgtctgct
     3781 gccgtggaaa tcggtgattg ataacgttgg gttaggcctt aaaggccagt ggcgcgatgc
     3841 cgcgcgtcgg gcgttggctg cagtaggact ggagaatcgc gccggggaat ggcctgccgc
     3901 actttctggc gggcagaaac agcgtgtggc gctggcaaga gcgttgattc atcgaccggg
     3961 tttattgttg cttgatgaac cgctcggggc gctggacgcc ttaacgcgac tcgagatgca
     4021 ggatttgatt gtgtcacttt ggcagcagca cggctttacc gtactgttgg tgacgcatga
     4081 tgtgagcgaa gcggtagcaa tggctgaccg ggtgttgtta attgaagagg gaaaaattgg
     4141 tctggatttg acagtggata tcccccgtcc gcgtcgatta gggtcggtgc gattagcgga
     4201 gctggaagcg gaagtattgc aacgggtaat gcggcgtggg cattctgaac aactgattcg
     4261 gcgtcatggt taatatgccg gatgtggcgc acaaaaccag cattttcaac ggactagtag
     4321 gcctgataag acacgtcaag catcgcatca ggcagttggg cacggctgcc ggatgcggcg
     4381 tgaacgcctt atccagccta caaaaccagc aatttcaacg gagttgtagg cctgataaga
     4441 cgcgtcaagc gtcgcatcag gcattttggc tcaaaagtat caagccagtg ctttggtgat
     4501 cttctcgtac agatcgccag agagattttc cagccctttc aactgttcca gcgccgcgcg
     4561 cattttctcc tgacgtttgg catcataacg tttcaggcga atcagcggtt caatcagacg
     4621 tgaagctacc tgcgggttac ggctgttgag gtcggtaagc atttccacca ggaactggta
     4681 accgctgcca tcttcggcat ggaacgctgc cgggttgctg cccgcaaacg caccaatcaa
     4741 cgaacgaata cggttcgggt tgctcatggt aaatgagcga tgctgcaaca gaccacgcac
     4801 cgtctccagc acattcgccg ccgggctggt ggcttgcagg ataaaccatt tatccatcac
     4861 cagaccgtcc tgatgccact tatcgtcgta ctcctgcatc agcgcgtcac ggcaaggcag
     4921 ctgtgcagca accgccgcag aaagcgctgc cagcgcatcg gtcatattgt ttgcttcatg
     4981 gtattgcttg ctcaccagca cgtcagccag atgcgtttca ccaaaagcaa ggaagcgcag
     5041 gcaggcatta cgcagagtgc gttttgcaat atcttcatgc tcaacacggt actcgctctg
     5101 gtaattcgcg ttgtaaatag ccagcagctc atccgccagt tcagtcgcca gagtacgagt
     5161 gagtgcttcg cgtacttcgg caatagcaat cgggtcgatg atatcgaaca gttcagccat
     5221 ttcattgaca gaaggcagcg tcaggatttc tgccgccagc gccggatcaa tcttctcatc
     5281 gagcagcacc gcgcggaaag cgtcagccac atgtaccggc agagacagcg gctgcccttg
     5341 ctgatgacgg gcgacgttca gcttgatgta ggttgccagc aaactttgcg ccgcatccca
     5401 acgggagaaa tcattacgcg catgacgcat caggaaggtc agttgctgat cgctccactt
     5461 atattccagt ttcactggcg cagagaattc gcacagcagc gcaggcaccg gctggaagta
     5521 gacattatca aaaacaaagg tctgttccgc ctgggtgacg ttcagcacgg aattcaccgg
     5581 atgaccgcct ttctgcaacg ggatcacttt gccttcgttg tcatacaatt cgatggcaaa
     5641 tggaatatgc agcggctgtt tttctgcctg atccggcgtg gctggcgtgc gctggctgat
     5701 ggtcagggtg tactgctcgg tttccggatt gtagtcgtct ttgacggtca caatcggcgt
     5761 gccggactgg ctgtaccaac ggcggaaatg ggagagatcg acattcgacg catcttccat
     5821 cgcctgcaca aagtcgtcgc aggttgccgc gctgccatca tggcgctcga aatagagttg
     5881 catccctttc tggaagtttt cttcgcccag cagggtgtgg atcatgcgaa tcacttccgc
     5941 gcccttctcg taaacggtca gggtgtagaa gttgttcatc tcaatgacca tatccgggcg
     6001 gatcgggtgc gccatcgggc tggcgtcttc cgcaaactgc aatccgcgca tggtgcgcac
     6061 attattgata cggtttaccg cgcgggaacc aaggtcagag ctgaattcct gatcgcggaa
     6121 gacggttaaa ccttctttca ggctgagctg gaaccagtcg cggcaggtca ctcggttacc
     6181 ggtccagtta tggaaatatt catggccgat aacgcgttca atatcgagat aatctttgtc
     6241 ggtggcggtg tcggtgcggg ccaacacata tttggagtta aagatattca gacccttatt
     6301 ctccattgcg cccatattga agaaatccac cgcgacgatc atatagatgt cgaggtcata
     6361 ctccaggcca aagcgttctt catcccattt catggagttt ttcagcgagg tcatcgccca
     6421 cggcgcgcga tcaaggttgc cgcgatcgac gtacagctcc agtgctactt cgcgaccaga
     6481 acgcgtggta aaggtgtcgc gcagtacatc aaagtcgcct gccaccagcg caaacaggta
     6541 gcacggtttc gggaacgggt cctgccactg tacccaatgg cgtccgtttt ccagttcacc
     6601 ttgcgcaacg cggttaccgt tggaaagcag gaagggatat ttggttttat cggcaataat
     6661 tttggtggta aaacgcgcca gcacgtccgg gcggtcgaga taatacgtaa tatggcggaa
     6721 accttcggct tcacactggg tgcaaagcgc atcgcctgac tgataaagcc cttccagcgc
     6781 cgtattcgcc gccgggctta tttcattaat gatcttaagc gtaaaacgct ccggcaaatt
     6841 actgatgacc agtgcgccct cttcttcttt ccaggcggtc cacggctcat cattaatatg
     6901 aacagaaacc agtttgaggt cttcgccgtt gagacgaagc ggagcatctg atgcaccatg
     6961 acggacagcc tggctgaccg cggtaacgac cgtcttttgc gcgtcgaggt caaaggtcaa
     7021 gtcaatatca gtaatctggt aatccggcgc acgataatcg tgacggtatt tggcttgtgg
     7081 ctgttgagtc ataaataacc tttagcatct tttatagagt ctggtgttca gtctattcct
     7141 gttgcgtaaa tcgcgctatg cagaatcttc atcttttcag gtacaaacgc ctttattgct
     7201 acatttttat aacatacacc gcgtaatgcc atcgaccaga aaggtggcat atggtgtgat
     7261 cggggttcaa taaattgcga aacaaggtat actccagcag ttcctgaaga tgtttattgt
     7321 actaaacgct cctgtacgag gacgctactg cgcacctatg acacaattcg cttctcctgt
     7381 tctgcactcg ttgctggata cagatgctta taagttgcat atgcagcaag ccgtgtttca
     7441 tcactattac gatgtgcatg tcgcggcgga gtttcgttgc cgaggtgacg atctgctggg
     7501 tatttatgcc gatgctattc gtgaacagat tcaggcgatg cagcacctgc gcctgcagga
     7561 tgatgaatat cagtggctct ctgccctgcc tttctttaag gccgactatc ttaactggtt
     7621 acgcgagttc cgctttaacc cggaacaagt caccgtatcc aacgataatg gcaagctgga
     7681 tattcgttta agcggcccgt ggcgtgaagt catcctctgg gaagttcctt tgctggcggt
     7741 tatcagtgaa atggtacatc gctatcgctc accgcaggcc gacgttgcgc aagccctcga
     7801 cacgctggaa agcaaattag tcgacttctc ggcgttaacc gccggccttg atatgtcgcg
     7861 cttccatctt atggattttg gcacccgtcg ccgtttttct cgcgaagtac aagaaaccat
     7921 cgttaagcgt ctgcaacagg aatcctggtt cgtgggcacc agcaactacg atctggcgcg
     7981 tcggctttcc ctcacgccga tgggaacaca ggcacacgaa tggttccagg cacatcagca
     8041 aatcagcccg gatctagcca acagccagcg agctgcactt gctgcctggc tggaagaata
     8101 tcccgaccaa cttggcattg cattaaccga ctgcatcact atggatgctt tcctgcgtga
     8161 tttcggtgtc gagttcgcca gtcgctatca gggcctgcgt catgactctg gcgacccggt
     8221 tgaatggggt gaaaaagccg ttgcacatta tgaaaagctg ggaattgatc cacagagtaa
     8281 aactctggtt ttctctgaca atctggattt acgcaaagcg gttgagctat accgccactt
     8341 ctcttcccgc gtgcaattaa gttttggtat tgggactcgc ctgacctgcg atatccccca
     8401 ggtaaaaccc ctgaatattg tgattaagtt ggttgagtgt aacggtaaac cggtggcgaa
     8461 actttctgac agccctggca aaactatctg ccacgataaa gcgtttgttc gggcgctgcg
     8521 caaagcgttc gaccttccgc atattaaaaa agccagttaa tatcatcagg gagctaatcg
     8581 gctccctttt tttaccttta attccgaaat ctttcgctgc atttgcgaat tctgcttgtc
     8641 tgattgcaga tgccaggtaa cataggtatc cccccattta cgggtggaca agtgtttatt
     8701 ttttccgact attaacagag agaatattat gagcgttgtg cctgtagccg acgtactcca
     8761 gggccgcgta gccgttgaca gcgaagtcac cgtacgcgga tgggtacgta cccgccgaga
     8821 ttcaaaagct ggcatctcct tcctcgccgt ttatgacggt tcctgctttg atcctgtaca
     8881 ggctgtcatc aataattctc tgcccaatta caatgaagac gtcctgcgtc tgaccaccgg
     8941 ctgctcggtc attgtgacgg gtaaagtcgt ggcgtcgccg ggccaggggc aacaatttga
     9001 aattcaggcc agcaaggttg aagttgctgg ttgggttgaa gatccagaca cttacccgat
     9061 ggcggcaaaa cgccacagca ttgagtatct gcgtgaagtc gctcacctgc gtccgcgcac
     9121 aaacctgatt ggtgccgtcg cgcgcgttcg tcatacgctg gcgcaggcgc tgcatcgctt
     9181 ctttaacgag cagggattct tctgggtttc aacgccactg attaccgcat ctgataccga
     9241 aggtgcaggc gaaatgttcc gcgtttctac gctggatctg gaaaacctgc cgcgtaacga
     9301 tcagggcaaa gtggatttcg acaaagactt ctttggtaaa gagtctttcc tgaccgtatc
     9361 tggccagttg aacggcgaaa cctacgcttg cgcattatcc aaaatttata ccttcggccc
     9421 gactttccgt gctgaaaact ccaacaccag ccgtcacctg gcggaattct ggatgctgga
     9481 gccggaagtg gcgtttgcta acctgaacga tatcgcgggt ctggctgaag ccatgctgaa
     9541 atatgtcttc aaagcggttc tcgaagaacg cgcagacgac atgaaattct tcgctgaacg
     9601 cgtagataaa gatgccgttt cacgtctgga acgcttcatt gaggccgatt tcgcgcaggt
     9661 ggattacacc gacgcagtga ccattctcga aaactgcggc aggaagtttg aaaacccggt
     9721 ttactggggc gtcgatctct cttctgagca tgagcgttat ctggcggaag aacactttaa
     9781 agcaccggta gtggttaaaa actatccgaa agatattaaa gcgttctata tgcgcctcaa
     9841 cgaagacggt aaaaccgttg cggctatgga cgttctggct ccgggcatcg gtgagatcat
     9901 tggtggctcc cagcgtgaag aacgtctgga cgttctggac gagcgtatgc tggaaatggg
     9961 cctgaataaa gaagattact ggtggtatcg cgatctgcgt cgctacggta ctgttccgca
    10021 ttcaggtttc ggtcttggtt ttgaacgcct gattgcttac gtaactggcg tgcaaaacgt
    10081 gcgtgatgtg attccgttcc cacgtactcc gcgcaacgcc agcttctgat tttcttcctc
    10141 aaagagccag cgcctgctgg ctctttttta tcctttctgt caagttatct gtttgttaag
    10201 tcaagcaatc tatttgcaac cccgccataa cgatcatcct gttacggaat attacattgc
    10261 aacatttacg tacaaaaaat aacccgcatt cttattgcgg attagttttt tcttagctga
    10321 tagcacaatt ttcatactat tttttggcat tctggatgtc tgaaagagga ttttgtgcca
    10381 gttcgataaa gtttccatca gaaacaaaat ttccgtttag ttaatttaaa tataagtaaa
//
LOCUS       DAZKGS010000147        10399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKGS010000147 DAZKGS010000000
VERSION     DAZKGS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(208..696)
                     /locus_tag="ACHOQY_003821"
     CDS             complement(208..696)
                     /locus_tag="ACHOQY_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9679833.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKSKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            complement(709..3516)
                     /locus_tag="ACHOQY_003822"
     CDS             complement(709..3516)
                     /locus_tag="ACHOQY_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9679834.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARKWYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(3503..3658)
                     /locus_tag="ACHOQY_003823"
     CDS             complement(3503..3658)
                     /locus_tag="ACHOQY_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9679835.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(3667..4032)
                     /locus_tag="ACHOQY_003824"
     CDS             complement(3667..4032)
                     /locus_tag="ACHOQY_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9679836.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGGKNEQATEA"
     gene            complement(4087..4599)
                     /locus_tag="ACHOQY_003825"
     CDS             complement(4087..4599)
                     /locus_tag="ACHOQY_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9679837.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(4599..5783)
                     /locus_tag="ACHOQY_003826"
     CDS             complement(4599..5783)
                     /locus_tag="ACHOQY_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP9679838.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEGGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            5941..7041
                     /locus_tag="ACHOQY_003827"
     CDS             5941..7041
                     /locus_tag="ACHOQY_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP9679839.1"
                     /translation="MTDTTMQLLSQSTDPVKMPDFDILAEGETLSGVAERLMSLSLTD
                     NRGFEADLLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSSNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPKKEKSSSREGDYMEGAEGNVFVLR
                     KTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSDIDNQDW
                     IIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            7441..8580
                     /locus_tag="ACHOQY_003828"
     CDS             7441..8580
                     /locus_tag="ACHOQY_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014606071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFP9679840.1"
                     /translation="MGNNKVKLGRNDKCWCNSGLKYKKCHLGRDRETPVSKAEALKFS
                     KSLSQRKACYAPASMHEDCDKRIVQAHTISKSSSLKAIADSSNHVMGVIMNLASLMRN
                     NGKWLPEKIGINQASTFTGFCAVHDRILFSCLENEKFTGTDEQCFALMFRSLSKEIYA
                     KEGGVLSSDFAKNADKGKLQVEQYYIQKLIDQHQSGLNAAIIELNNLKTSLDRILLSR
                     QFSEIESVIFTFAEPLPIVVSSILSPERDFDGTKIQDLSDLTVSAEQVCFNAFSCGGK
                     GYVVFSWLRTSTIIRRFIRTLIKIPANRMFNTLLYFFFTKAENTYSSPEWWDRLSDKQ
                     RENIGNMIMSGVEFFGRPTSQVDHSVDYNAAALEEITCSNNEICS"
     gene            8867..9127
                     /locus_tag="ACHOQY_003829"
     CDS             8867..9127
                     /locus_tag="ACHOQY_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9679841.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            9318..9458
                     /locus_tag="ACHOQY_003830"
     CDS             9318..9458
                     /locus_tag="ACHOQY_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9679842.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLRGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(9647..9928)
                     /locus_tag="ACHOQY_003831"
     CDS             complement(9647..9928)
                     /locus_tag="ACHOQY_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679843.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRNGGYDVVCYEGDVTDEMIRQWKQENAE"
BASE COUNT         2661 a         2785 c         2483 g         2470 t
ORIGIN      
        1 agtagagtcc ccaaataaaa caaagtgaat tgccattaag taagacagca cgataatctc
       61 cttaattatt atttcagacc acacatatta taaggttaag agattataaa atcctgttgt
      121 ttgttattca aaaacaattt tctgagaagg acatacaaca gcaagtcgcc agtcaccttc
      181 atcaggaaat tggcgacata cgttaaatca gagcagcccc ttaactgagc tggtcgcgct
      241 attaagggat gatgtcacct tatctttgaa gccggacaac atatcgctga acgatgagga
      301 ttgcaggcgc tcccgcaaat cctcatcaca gcgttcaaga gtcagtgaaa attctatctt
      361 tttcgactta ccgtagcgat caaactcgga gcgggtcgta ttcgtttcgg tcaggacata
      421 catgccgtaa atctgcccga cgccatcaat cagaggccag ggccgtcctg tatacgcctg
      481 cgtggtcagc agagacagcg acacttcgcc acctgtaatt tcaggataaa gcacaccaga
      541 aagaacgatg cgatcatcac ctgcaccgat atactgccag cttgctgaac ggttaacgcg
      601 ttcatttttc acatgccgcc agcttttgtt ttgctgtaac tgctgatgcg gcagcgtgcg
      661 cagctcaaaa acaaacatgc catagatcat catcatggcc atgactcctc aatctttatc
      721 gtaaaaactg ccacgcccgg cacgggcgcg ccgttccatt tctgccctga ccatttcacc
      781 gaccagtttc gccagttcgc ggggattctg cgtaacaacg ttatgcagat gaacatgaat
      841 ttcaccgcca aatctggaga cagcaggctc ccgattacgg gaagctgcag gaactgatgc
      901 cactggcgat cgtatggcct ccgccaccgg gcgggagctg gccgcaacaa cagggaccag
      961 cgccggaggc agcggagccg ggactacggg tgtgatatta attgcggggg caggcttact
     1021 gacctgcgca atcttccgct cctgccactc cccacgaaca gcaagtgcgc ggggcaggtt
     1081 cttaaagaca atatcgccag ggccaatgcg ttttttcgtc tcatcaacca gcttacctgt
     1141 gttatcagca attttgctga gtctgcgtag cgtcccggta ttgctgtctg tgagcggttt
     1201 gttgtctttg ggtttatcac ctccggtgcc attgccattt tccacaggct tcggcggatt
     1261 gattttcgcc aggtccccct gaagtaaggc aaccttatcc tgaagaatgg ccgcacgctg
     1321 tgcgtcttcg attttcttgc gcgccctttc cgcttcatcc ggaaggacgc caagtttttc
     1381 aagtatccac gccagcgtat ccagtagcat ttttgcaggt gtcagaacaa gttgtaacgc
     1441 accgccaaga acgttaccga atatctcgcc agcactggta catttatcca acgtttcctt
     1501 gctggactcc atcggtgaca gcagcgattt aaaccagtta aacacctggc tgatcccgct
     1561 tccgattgcg tcaaaaacag ggccaaaccg ttcaaaggtt tcacgcaacg ggttcagcct
     1621 ttccataatc ccgctgaaca ccccggcaaa aaatgccctg atgggatccc agtatttcca
     1681 gataaggacg gcagctccgg caagcgcagc cacgataaga ccaaccggac tgaacagcgc
     1741 cccgatagcg cctcccagta aagaaacgga acccgtcacc attccccata gtgctggcag
     1801 gaccctgaca gcattcattg atccggtcag gagagaaaaa ccaagacgca gttttgccag
     1861 cggaccagca agcacaccaa tagccagcga caacgagcca accgttgcag tcattgccag
     1921 caacgcaccg cctgctatca gtagctggcg cgtcagtgcc ggatgggcct gcgccagcgc
     1981 cgtcaccctt gataccaccc gcgtgagcca ctgcgtgaca gaacgcagcg gaccgtcaat
     2041 cagatctgca atgcggatgc gcaacccttc ccatgcactg ccgagtgatt tcagatcgcc
     2101 gtcaaggttg ttggccataa cctttgctgt gcgttcagcc tcaccgcgcg cgccttcaag
     2161 ttcttttctc agtttgggta aggaaccgtc acccactgca tcaacgagcg ccataaacga
     2221 tgtgaaagcc tcttctccgg caatgtcctt aaagaacgat acccggtcaa cttccccgta
     2281 tttgcgggtg gctttataaa ggtcggccag cacatcctcc atcgggcgca ttttaccccc
     2341 ggcatccgag acggacacgc ccagctcttt cagagcttct gccgccgcct ttggcggtga
     2401 tgccagacga gccaggctgg cacgcattgc cgtcccggca tcactccctc tgatgcccat
     2461 attcgccagc acaccagcca tcgctgcggc ctgctccagc gatattccca gcttacccgc
     2521 caccggacct gcatatttca tggtttcgcc cagtgcgcga aggtcagtgt tggtacgggt
     2581 aaacgctgcg gtgagtgtgt caccgacccg gtccatctgg tcagcagaaa ggccgaactg
     2641 cgtcaggata tttgagccaa tatctgccgt ctcgccgaga tccataccgc cagccgttgc
     2701 catgctcagc actccgggaa gcgcagcctg aatggcctgc ggtgtgaagc ctgccattgc
     2761 aagaaatgcc tgcccactgg cggcatcgcc tgcggtgaac tgcgtttcag agccaagttt
     2821 taacgcctgc tcacgcagcg ccttaaactg cgggctgttc tggtcgattc gcgtcagcgc
     2881 ctgaacgcgg gacatctctt tgccgaaccc gatcgcgggc tgcaaaaaac gcccggcagc
     2941 atagccgccc gccgctgccg caccaattgc cagcgcacca cctgttttca gttttcccgc
     3001 ggtttcctgc gcgcgcgaat accacttacg cgcccgcgtt acacgcgcaa gcgcctgccg
     3061 ttcgcgttca agctggttgt tgtactgttc ggtgcgtctg atggcctgct ggatggtgtt
     3121 atcgctgcct gtcagggaaa tgccgtggcg tttcagctct ccgccaagct cccgcatttt
     3181 ctgaatttcc cgtgtgcgcg attcattcag gcgttcaagc cgggtgctta actgctgcat
     3241 cagcttttgt tgtttttcgc tgagcactgt acccgtgcgt tgtaactgat taagggcgtt
     3301 aagctggcgt cgtgctttca cgatacccgc atccgcttta ctgacagcgt cgcgggcgcg
     3361 ctcaaatgaa cgagcctgac gctcgagatt tttaatcgcc ccctgcgttc gctggatgga
     3421 gtcaccaaac tgccccatca ggcggcgggc gttttcggca ggccgggtca gcctgtcaac
     3481 ggcgctgaaa gcgacccgga tatcaagagt cttcattgtc tgcattcccg ctgcgaagtg
     3541 ccgcccgctc acgccagcta accacttcgc cgggcgtcat catgaagatt tcggcgggcg
     3601 accagttaaa aataacggca atatctgcca caaagtcttc tatgtgctca aagcacacaa
     3661 ccgtgatcag gcttccgtcg cctgttcgtt cttcccgcca gagtccgcac cgctcaaaaa
     3721 atttacggca accacacata actgaataaa gtcacgggat gccatttttt tgatcgtcac
     3781 ttcatccagt cgcggtgatg tcacgcgtga cagcagcgta aacatggatt ccgctttcag
     3841 attcagcaca tcagacagcg acaaatctcg cagagatcca gcctgctcaa tagccccggt
     3901 gatctccaca tacgtgattt tttcgccgcc tcgctcaatt ggttgggtaa gttttacacc
     3961 acgctcactg gtttctttca cagtgtcagc aactaccgtg ttttcggtat cgatgttttt
     4021 cgtctctttc atcaggaaac tcctttcagt cagaggcgac gcactgcgcc gcctgcatat
     4081 tacttatcag ccaagcccga gcgcggaacg gatgcgatcg ggcacaatgt ccttgccgtc
     4141 cttccggtaa atgaagttca gcaggtcaat ctcccacaac gggcgatcgt taacactcag
     4201 cttgtagtag gtgtttttaa tggcgtaagt gtgtgatgtg gcttcgccct gtttggcttc
     4261 ccccatatca atttccgtca cacgtccgcg catttcgact tcatacaggt cgctttctgc
     4321 atcggtgtaa tattcacccg caaaacgcag cagcgtgccg tcaatcgtgc cgccatactt
     4381 aaggaacagc tcacgaactg cgccccccat gacaaagctc gcatcaagcg cggagtcgtc
     4441 cagaccgaga tcaatactta ccgcacccat catgccacca ccccggtagc tgtcggtttt
     4501 gcgcgtcagc ttaggcagag tgacggacgt caccttaccc acttcgtttt caccatccac
     4561 aaacagcgta aaaaagcgaa gatgttttgg tacagccatc aggcacctcc cagcaccgca
     4621 aatgcgggtt caaagtattc atcagtaaac gtctggtaaa gctccatgtc ttccagtggc
     4681 ggaacgggcg tatatttgta gcgaatacgc acacgcccct gacgtaaatc cgtggtgctg
     4741 ttatccacca cgtcatacca gcactccgcg ccaatcagtt tcccggcagt aaccagcgaa
     4801 tccagttttg ccctgatggc actgataaca tccttcacgt tcgcaggcgt cagtggactg
     4861 tcgatggttt caaactgcgc ttccgcaatt gaatcagcca gcacctgtgc ggttcgggta
     4921 tacacctcaa agatgtaggc gttcgtttcc ggtgtgcggt tgccccagaa gcggaacccg
     4981 ttgcgacgaa taatggtcgt gatttctttg ttgttgaggc tgttggcatc actgtcttcg
     5041 gcctgcaacg accagaaaac atgcctggac atccccagca catttttaac aggaacgttg
     5101 gacagcgatt tgtgccagcc ctgctcatgg tcaatgtacg cacgaaggcc gcacgcatag
     5161 gcaggcgcgg ggaacgtttc gtttttgcca cttttcgggt tgtaggcgat gaagtccggc
     5221 cataagagca tcacctcacg ttcgttgaat ttctggcggt aggtaattgc ctcagccatc
     5281 gtgttacagt catgacatga ggcatacaca aacgcgcgca gtttacccgc aatcacgcac
     5341 agggattttg tcaccgcctc cgtgtccagc tccggcgcgg ccagaatacg cggacggtat
     5401 ccgatgcttt catcctgctc tgcaacaagc agcgcataca tccccgtata gctgccgtca
     5461 gattcagaac caccgataac cagttgatcc tgcgtcttac cgccttcttc tttgtgttca
     5521 gccacgcgaa cgacgatcac ttttgtgctc acctggtctg caatagcctt aagcgcacga
     5581 taaagcgtcc ccgtagtccc gcattttccc agcacgtcat tgacgcgggt cagcagtgtg
     5641 ggcttgttca gcgggaacag ctccgcgtcc gcatcatccg ccgttgccac gataccgata
     5701 acactggaat caacatcatt aatcgctgtt accaggtcgg tactttccgt aacacgggca
     5761 ccatgaaaac gagtttcact catagcttca gccccttgta tccgttaaat gattcggcaa
     5821 caatcatcac ccaccacgcg cgtaatctca cccctgcgcc gttctcccgc cacggcgaca
     5881 acaaaaagca gtaaccccct atgcacgcac atgcgaccat gccgcacagg gagggaacag
     5941 atgaccgata ccaccatgca attgctcagt cagagcacag accccgtgaa aatgccggat
     6001 tttgatattc tcgcggaggg ggaaacactg tccggcgtgg cagagcgcct gatgagcctg
     6061 tcactgaccg acaaccgggg atttgaagcg gacctgctca ccatcacgct ggatgatgcg
     6121 gatggtcagt tgcagctacc gccacggggc gcgcgcctga cggttctcat tggctggaaa
     6181 ggagaaccgc tgacagaaaa aggcacttac attgttgatg aaatcgctca cgaaggaccg
     6241 ccggacaggc tgactgtttc agccagaagc gcagattttc gggatgaatt taacgttaaa
     6301 cgtgaggtgt cctggcatga tgtgaccgtt gagcgcgtgg tatccgccat cgctcatcgg
     6361 tacggtctga aaccacaaat cagcgaaatg ctgatggata ttgaaatcga ccacgccgac
     6421 cagaccgaag aaagcgacat gtccttcctt acgcgcatgg cggaaatgct gggcgcaatc
     6481 actacggtaa aaagcagtaa tctgttattc atcatgccag gcggtggcgt gaacgcacag
     6541 ggccagccgt tgccatcgtt cgccatcacg cgcagcagtg gcgatcgcca tcagttccgc
     6601 attgctgacc gcgaagcgta tacgggggta cgtgcttact ggcttgatct taattacggg
     6661 aaaaagaaaa aagtcagcgt gaaacgccgc aaaccgaaaa aggagaaaag cagcagccgt
     6721 gaaggtgact atatggaagg tgcggaaggc aacgtgtttg tgttacgcaa gacttatcag
     6781 aacgagcagg cagcaagacg tgcagcggcg gcaaagtggc agcagctaca acgcggagcc
     6841 gcatcattct ccatcacgct ggcgcgtgga cgcgcagaac tctatcccga aatgcatggc
     6901 acggtgacag gcttcaaaag tgacatcgat aatcaggact ggattattgc aaaagccgag
     6961 cacaccattg ataacagcgg ctttaccaca cagcttgagc ttgaagcaaa aatcccggaa
     7021 tggatagcag aaacagaatg agcaaattag atgtattgac tcaaaccaga actgacacac
     7081 tcacagcaca gtgccagacc caatctggca gcccgctttg tgcctagagc aggtgttgct
     7141 tatgtaacag ttaatccacg gatagtggtc atgaatgctg gttgaagttg tattgccatg
     7201 agtggtcaat tagggactca cttattatcg cgtgctcacc attgatgggt aagatcatgc
     7261 atttcggcta ctgtacttcg cgaatcctcc gcacacaggc aacacctgat ttaagaacag
     7321 agggagatga tgacgcaagg aagatttatt atcgtgcatg tggcagatga tcttttagct
     7381 gccggagtga taaattacac ctattttcga taagtgtttt tgttatagaa cattaatttc
     7441 gtgggaaaca ataaagtgaa attaggaaga aatgataagt gctggtgtaa ttcaggactt
     7501 aagtataaaa agtgccatct tggtcgagat cgtgaaactc cggtatccaa agccgaggca
     7561 ctgaaatttt caaagtctct ctcgcagaga aaggcatgtt atgcgccagc atccatgcac
     7621 gaagattgtg ataaacgcat cgttcaggcg catacgatat ctaagagcag tagccttaag
     7681 gcgatcgccg actccagcaa tcacgtcatg ggggtgataa tgaacttagc aagcctgatg
     7741 agaaacaacg gaaaatggct tcccgagaag atcggtatca atcaggcatc cacttttaca
     7801 ggattttgtg cagttcatga cagaatctta ttttcatgcc tagagaacga aaaatttaca
     7861 ggcacggacg agcagtgctt tgccttaatg tttcgttcat tgtctaaaga aatttatgcc
     7921 aaagaagggg gagtgctatc ttctgatttt gctaaaaatg ccgataaagg aaagctacaa
     7981 gttgaacagt attatattca gaaattaatc gaccagcacc aaagtggctt aaatgcagca
     8041 ataattgaat taaataatct taaaactagc ctcgaccgca tcctacttag caggcaattt
     8101 tcagaaattg aaagtgttat cttcactttt gcagagcctt taccaattgt agtttcgtca
     8161 attctttcac cggagaggga tttcgatggg accaaaattc aggatttgag cgatcttaca
     8221 gtgtcggcgg aacaagtatg tttcaatgct ttttcctgcg gaggtaaggg atatgtagtg
     8281 ttcagttggc tgagaacttc aacaattatc agacgtttta tacggacact aattaagatc
     8341 ccagctaaca ggatgttcaa tacccttctg tattttttct tcacaaaggc cgaaaataca
     8401 tattcatccc ctgagtggtg ggacaggctc agtgataaac agcgagagaa tatcggcaac
     8461 atgattatgt caggggttga attttttggc agacctacat cacaggttga tcattccgtt
     8521 gattataacg ccgctgcact tgaagagata acatgcagca acaacgaaat ttgcagctga
     8581 atgtttacat gctgttagaa aaagcgggct gaacaatcat ttcgggattg taacagataa
     8641 gatggcactg cccgcttgct tcccgatatg ctcagccaat aaacttatgt aagcaaccga
     8701 tttccattta tgcggaggtt atcaaagttg ttggagttgc gaacttccgc tcttcgcaca
     8761 aagcgtaccg ctaggtttga ttgtattctg gctgcgcaaa ctgtcagttg gagtctgagc
     8821 tattacaact tagaatagcg gcagcaccac gttaagggag gtcgctatgt tccgttgtcc
     8881 gctttgtggc gcatctgccc gaatccgcac cagtcgtccg gaaaacgatt caaacactgt
     8941 gcggcaaaag tattaccagt gtaacaacct ggaatgcggc gtatgcttct caacactgga
     9001 agctttccat aaattcacat caaaacacgc ctccggcgtt cactcttcgg aaggtatccc
     9061 gtggcatgag ctgccagctt cacacagggg aaacaatcag atgagtttgc ctttacctca
     9121 gaattaacag gcagaattgc cggagtaaca aaaaaacgat agattacccg cgggtgcctt
     9181 tcggctgatg gtcggaggaa tacccgaagg ccggatgtgg aaaggccccg gaaaacattt
     9241 ctgtttaacc gaggccctaa catatctacc ttaagcaagt gataggttag cgcctctcca
     9301 acaaaggagc aagcgctatg tcgcaaaaat cgcttacagc catcacgttc tgcgtgacgg
     9361 taatcctcat catctggatg ctacgcggtt cgctgtgtga aatacggatg agcttctggg
     9421 gagcggagtt tgcggcgttc ttacagtgta agcagtaagg aaaccgcgac gggggagcaa
     9481 tcccccgtca atcggttgct aaggttggct gaagtggcac cctatctcac agacatgaac
     9541 aacaaacccg cagcgtaaga actgcgggtt ttctttttgg aacaatcact agctagtgta
     9601 ccgttaacgg cacataatgc tcgcacaaca aacctgttaa aagagattac tcagcattct
     9661 cctgcttcca ttgccggatc atttcatcgg taacatcacc ctcataacat accacgtcat
     9721 accctccatt acggctatat gcactgcgtc caccacatct actaccattc cttgcatgat
     9781 tataaggaca cgcacaattg cctggataag attcaatgga ttctttaatt atttcttttt
     9841 tgatctgagc atcagacttt cctgttgctg catatccact aaaagatact aaacaaaggc
     9901 agattgccat taataaacca catcgcatac cgatacactc tattactaac caatcatagc
     9961 ccacatcata aaatcgagat gaggagtaca atacctttaa caagaaggtg tcataacgat
    10021 aatatgtcgt gcaattcatc aaaaaaaaga tctatatcaa agagatagaa ctcttatcag
    10081 tgcttcagac atcggcattc gaggcagcag aaaaacagta aatctgacga atttgccttt
    10141 tcccaagatg ttgtcagatt aaaaagatag attagcgcag gtacctgttg gtggattgca
    10201 ggaggaaaaa tgaagtcgag tgaaaaaatc ccagaaaaat ttttgtttaa cagaggtcct
    10261 ggttcactat gcgaaatacg gaggagctgc agaagaattc tgcttcatcc ttagagttta
    10321 aacagcacgt gcactaccat tgtttctgca tatcgccaca ccatcgccac tctaccgcca
    10381 ttgcacaaaa tacaaatac
//
LOCUS       DAZKGS010000148        10350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKGS010000148 DAZKGS010000000
VERSION     DAZKGS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..1085
                     /gene="dusB"
                     /locus_tag="ACHOQY_003832"
     CDS             120..1085
                     /gene="dusB"
                     /locus_tag="ACHOQY_003832"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9679844.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1111..1407
                     /gene="fis"
                     /locus_tag="ACHOQY_003833"
     CDS             1111..1407
                     /gene="fis"
                     /locus_tag="ACHOQY_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9679845.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="ACHOQY_003834"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="ACHOQY_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9679846.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="ACHOQY_003835"
     CDS             2461..2640
                     /locus_tag="ACHOQY_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9679847.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="ACHOQY_003836"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="ACHOQY_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9679848.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            3704..4861
                     /gene="acrE"
                     /locus_tag="ACHOQY_003837"
     CDS             3704..4861
                     /gene="acrE"
                     /locus_tag="ACHOQY_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9679849.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4873..7966
                     /gene="acrF"
                     /locus_tag="ACHOQY_003838"
                     /pseudo
     CDS             4873..7966
                     /gene="acrF"
                     /locus_tag="ACHOQY_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            8219..8440
                     /locus_tag="ACHOQY_003839"
     CDS             8219..8440
                     /locus_tag="ACHOQY_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679850.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            8871..9896
                     /locus_tag="ACHOQY_003840"
     CDS             8871..9896
                     /locus_tag="ACHOQY_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9679851.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            9964..>10350
                     /locus_tag="ACHOQY_003841"
     CDS             9964..>10350
                     /locus_tag="ACHOQY_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9679852.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIE"
BASE COUNT         2634 a         2432 c         2572 g         2712 t
ORIGIN      
        1 ttttaaatgc aattctttga tccatctcag aggattggtc aaagtttggc ctttcatctc
       61 gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag tatggtcatt tcttaactca
      121 tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc agcgcccatg gctggcatta
      181 cagacagacc ttttcggacg ttgtgctacg agatgggagc cggattgaca gtatccgaga
      241 tgatgtcttc taacccacag gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca
      301 ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag cgatccgaaa gaaatggcag
      361 atgcagcacg tattaacgtg gaaagcggtg cccagattat tgatatcaat atgggttgcc
      421 cggctaaaaa agtgaatcgc aagctcgcag gttcagccct cttgcagtac ccggatgtcg
      481 ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt tcctgttacc ctgaagattc
      541 gcaccggctg ggcaccggaa caccgtaact gcgaagagat tgcccaactg gctgaagact
      601 gtggcattca ggctctgacc attcatggcc gtacacgcgc ctgtttgttc aatggagaag
      661 ctgagtacga cagtattcgg gcagttaagc agaaagtttc cattccggtt atcgcgaatg
      721 gcgacattac tgacccgctt aaagccagag ctgtgctcga ctatacaggg gcggatgccc
      781 tgatgatagg ccgcgcagct cagggaagac cctggatctt tcgggaaatc cagcattatc
      841 tggacactgg ggagttgctg cccccgctgc ctttggcaga ggttaagcgc ttgctttgcg
      901 cgcacgttcg ggaactgcat gacttttatg gtccggcaaa agggtaccga attgcacgta
      961 aacacgtttc ctggtatctc caggaacacg ctccaaatga ccagtttcgg cgcacattca
     1021 acgccattga ggatgccagc gaacagctgg aggcgttgga ggcatacttc gaaaattttg
     1081 cgtaaacaga aataaagagc tgacagaact atgttcgaac aacgcgtaaa ttctgacgta
     1141 ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc aaaaacccct gcgtgactcg
     1201 gttaaacagg cactgaagaa ctattttgct caactgaatg gtcaggatgt gaatgacctc
     1261 tatgagctgg tactggctga agtagaacag cccctgttgg acatggtgat gcaatacacc
     1321 cgtggtaacc agacccgtgc tgcgctgatg atgggcatca accgtggtac gctgcgtaaa
     1381 aaattgaaaa aatacggcat gaactaattc aggttagcta aatgcttgat taaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccggt ttggtaatga agctaagacc attattcacg gtgatgcgct
     1561 tgccgaactt aaaaagatcc ccgccgaaag tgtcgatctg atctttgccg acccaccata
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta
     1741 catcatgaac agcacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttctgga gtacaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa
     1921 cggtgatgct attctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg
     1981 caaaaatcct ccacagccat acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 gcgcgtgcgt tatttgatgg atgaatatga gaaccacccg acgcaaaaac cggaagcctt
     2101 actgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 cggtagcttt actaccggtg ccgtagccgt ctccagcggg cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc aacctgtcaa aacgaagccg
     2341 gttaagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatacaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctggca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttacatga cacttaattc attcgtttga tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccaga gcgggggctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccattaat aataatcatc
     2821 acaacatcta aatcgaggtt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagag tctgcggatt gaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaagcc gacaatcaat ttttcacgca attgttgaaa cgggtcatgc
     3061 tctaatccag ccgtcaagtg ttcctggatt aactcccgca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgagt cttgttttcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcagcgt cggcaatgtc gttgagtgtc gtcttgctta cgccatgctg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcagag cttcggcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca gagtgagaaa atagcgaagg
     3361 ttaatctatc acctaatgtg tatttatacg agaggctaat attgagttgc tataaatcgt
     3421 caaataaata atatatatta tttacctaaa atacattcgc cacatgaata tatatttcaa
     3481 tttacgaggt tttaattctg cctctttcaa cccgcgtcaa aataaaacag tagaatatta
     3541 atcttttttt gtgtttatgt gccttgagac gccagtattc ataactattc cttacatcga
     3601 cgaatgataa tttgtaggat agcaaactgt atttttcttt ctgcgagtta acgcgttacc
     3661 tttctgggta aataacgcgc ttttggtttt ttgaggaata gtaatgacga aacatgccag
     3721 gtttttcctc ctgccctcct ttattctgat ctccgcggct ttaatcgccg gttgtaacga
     3781 taagggagaa gagaaagctc acgtcggtga accgcaggtt accgttcata ttgtgaaaac
     3841 ggccccgtta gaagttaaga ctgaattacc aggccgcacc aatgcttacc gtatagccga
     3901 agttcgccca caggttagcg ggatcgtact gaatcgtaat ttcactgaag gcagcgatgt
     3961 acaagcaggc cagtccctgt accagatcga tcccgcgacc tatcaggcaa attatgacag
     4021 cgcgaaaggc gaactggcga aaagtgaagc cgccgccgcc atcgcgcatt tgacggtaaa
     4081 acgttacgtt ccgctcgtgg gtacgaaata catcagccag caggagtacg accaggccat
     4141 tgctgatgct cgccaggccg atgccgccgt gattgccgca aaagccacag tcgaaagcgc
     4201 tcgcatcaat ctcgcttata ccaaagtgac tgcgccaatt agcggacgta tcggcaaatc
     4261 gaccgtgacc gaaggcgctc ttgtcactaa tgggcaaacg actgaactgg cgactgtcca
     4321 gcagctcgat cctatctacg ttgatgtgac ccaatccagc aacgatttta tgaggctgaa
     4381 gcaatccgta gagcaaggaa atttgcataa ggaaaacgcc accagcaacg tagagttggt
     4441 catggaaaac ggtcaaacct atcccctgaa aggtacgctg caattctctg atgtgaccgt
     4501 tgatgaaagc accggctcca taaccctacg tgctgtcttc cctaacccgc aacatacgct
     4561 tttgccgggt atgtttgtgc gtgcacggat tgatgaaggc gtccaacctg acgccattct
     4621 tatcccgcaa caaggcgtta gccgcacacc gcgtggtgat gcaaccgtgc tgattgttaa
     4681 cgataaaagt caggtggaag cgcgccctgt cgttgccagt caggcgattg gcgataaatg
     4741 gttgattagc gaaggtctga aatctggcga tcaagtcatt gtcagcggcc tgcaaaaagc
     4801 gcgtccggga gagcaggtta aagccactac cgataccccc gcagatactg catcgaagta
     4861 aggtaatctg acatggcaaa cttttttatt cgacgaccga tatttgcatg ggtgctggcc
     4921 attattctga tgatggcggg cgcactggcg atcctacaat tgcccgtcgc tcagtatcca
     4981 accattgcac cgcctgcggt ttctgtttcg gcaaactatc caggtgctga tgcgcagacc
     5041 gtgcaggata cggtgacgca ggttatcgaa cagaatatga atggtatcga taacctgatg
     5101 tatatgtcct ccaccagcga ttccgccggt agcgtgacaa ttacccttac cttccagtcc
     5161 gggaccgatc ctgatatcgc gcaagtgcag gtgcagaaca aactccagct cgccacgccg
     5221 ttgctgccgc aggaggttca gcagcagggg atcagtgttg aaaagtccag tagcagctat
     5281 ttgatggtgg cgggctttgt ctctgataac ccagacacca cacaggacga tatctcggac
     5341 tatgtggcct ctaacgttaa agatacgctt agccgtctga atggcgtcgg tgacgtacag
     5401 cttttcggcg cacagtatgc gatgcgtatc tggctggacg ccgatctgct aaacaaatat
     5461 aaactgacac cggttgatgt gattaaccag ttgaaggtac agaacgatca gatcgctgcc
     5521 ggacagttgg gcggaacgcc agcgttacca gggcaacagt tgaatgcctc gattattgct
     5581 caaacgcggt taaaaaatcc ggaagaattc ggcaaagtga ccctgcgcgt aaacagtgac
     5641 ggctcggtgg tacgcctgaa agatgtcgca cgggttgaac ttggcggtga aaactataac
     5701 gttatcgctc gtatcaacgg aaaaccggcg gcgggcctgg ggattaagct ggcaaccggc
     5761 gcgaatgctc tcgataccgc gaaagccatt aaggcaaaac tggcggaatt acagccattc
     5821 ttcccgcagg gaatgaaggt tctctaccct tatgacacca cgccattcgt ccagctttct
     5881 attcacgaag tggtaaaaac gctgttcgaa gccattatgc tggtgttcct ggtgatgtat
     5941 ctgttcttgc agaatatgcg agcaacgctg atccccacca ttgcggtacc cgtggtgttg
     6001 ttagggacgt ttgccatcct cgccgctttt ggttactcca tcaacacact aacgatgttc
     6061 gggatggtgc ttgccatcgg gctgctcgtc gatgatgcga tagtggtggt ggagaacgtc
     6121 gagcgcgtga tgatggagga taagctcccg ccaaaagaag cgacggaaaa atcaatgtcg
     6181 caaatccagg gcgcactggt gggtatcgcg atggtgctgt cagcggtatt tattccgatg
     6241 gcattcttcg gcggttctac tggggcaatt tatcgccagt tctctatcac catcgtttcg
     6301 gcaatggcgc tttctgttct ggtggcattg attcttaccc ctgcgttatg tgcaacgctg
     6361 cttaaacccg tctctgctga gcatcacgaa aataagggcg gtttcttcgg ctggtttaat
     6421 actaccttcg atcatagcgt taaccactac accaacagcg tcggcaaaat cctcggttcc
     6481 acaggacgat attgcgctga ttgttgcagg aatggtggtg ttgtttttac gtcttccgtc
     6541 ttccttctta cctgaagagg atcagggtgt ctttctgacc atgattcagt tacccgctgg
     6601 cgcgacgcaa gagcggacgc aaaaagtgtt ggatcaagtg acggattact atctgaagaa
     6661 cgagaaagcg aacgttgaaa gtgtctttac ggttaacggc tttagcttca gcggccaggc
     6721 acaaaacgcc ggtatggcct tcgtcagtct gaaaccgtgg gaagagcgta atggtgatga
     6781 aaacagtgcg gaagcggtaa tccatcgtgc caaaatggaa tttggcaaga tccgcgacgg
     6841 ttttgtcatt ccattcaata tgccagccat tgttgaactg ggcacggcaa cgggtttcga
     6901 ctttgagtta attgatcagg ctgggctggg tcacgatgcc ctaacccagg cccgtaacca
     6961 gttgcttggt atggcggcgc aacatcctgc cagcttagtc agcgtgcgtc ctaatggcct
     7021 ggaagacacc gcgcagttta aactggaagt tgaccaggaa aaggcgcagg cattaggtgt
     7081 ttcactttct gacatcaatc agaccatttc aacggcgttg ggtgggactt acgttaacga
     7141 cttcatcgac cgtggtcgcg tgaaaaaggt gtatgttcag gcggatgcca aattccgtat
     7201 gctgccagaa gatgtcgata aactttatgt ccgcagcgcc aacggcgaaa tggtgccatt
     7261 ctcggccttt accacttcac attgggtgta tggctctccg cgactggaac gctacaacgg
     7321 tctgccgtca atggagattc agggggaagc cgcgccagga accagttccg gcgatgccat
     7381 ggcgttgatg gaaaaccttg cgtcaaaatt acctgctggc attggttatg actggacggg
     7441 tatgtcgtat caggaacgct tatcgggaaa ccaggctccc gctctggtag caatttcctt
     7501 tgtggttgtt ttcctgtgcc ttgctgcact ctatgaaagc tggtcaattc ctgtctcggt
     7561 tatgttggtc gtgccgttag ggattgtcgg cgtgctgctg gcggcgacac tctttaatca
     7621 aaaaaatgac gtctacttta tggtgggctt gctaacgaca attggcttgt cggccaaaaa
     7681 cgctattttg attgttgagt tcgctaaaga tctcatggag aaagagggta aaggtgttgt
     7741 tgaagcgaca ctaatggcag tacgtatgcg tctgcgtcct atcctgatga cctctctcgc
     7801 ctttattctc ggcgtattac cgctagctat cagtaacggt gccggcagtg gcgcgcagaa
     7861 cgcagtgggt atcggggtaa tgggcggaat ggtctctgca acgttgctgg caatcttctt
     7921 cgtaccggtg ttctttgtgg tgatccgccg ttgctttaaa ggataaatca gaaacataaa
     7981 ggcactttcg ggtgccttta ttattttcag tgaaacccat aaaaattaat aagatattct
     8041 tccactcact ctttaaaaac tttctatagt tcccgctccc ttcactattt ttacaattca
     8101 cataatttgt cattattcat tccgcaatat ttataacctc cggtgataaa atggcattca
     8161 gctcgttaat aagagagtta acttattaag cgttagcgtt tattactgag gtaacaccat
     8221 gaaaagatta attcctgtgg cattgctcac cgcattgctg gcgggctgtg ctcacgattc
     8281 gccctgcgtt ccggtttatg acgatcaggg tcgtctggtt cacaccaata cctgtatgaa
     8341 aggcacgact caggataact gggaaacggc tggggctatt gccggcgggg cagcggctgt
     8401 tgctggcctg acgatgggga tcatcgctct gtcgaaataa ctgaacaggg cgcggagttt
     8461 cgcgctctgt ttaacgatac agtatcttta cataagataa aatattgcta tattctgctg
     8521 gctaattctc tgctgattta gcatgttata tcgcgttatt tcgctgcttt cgaaataacg
     8581 catcaattta actttcacac ttcccaccag aaatatactc acgctaacaa tcattattct
     8641 gcttagtaaa acacgcgtaa atattttgcg ccaaaatgtg gcgcatgttt cattttcgca
     8701 ccattgcggg gcgctgtttt tattatttcc tgtctacact cagcttattg cgacgcgaaa
     8761 tcgggcgcaa aagctggcac tacttttgct tataagagga tggccacaga caggtaaaag
     8821 acgtttccca aacgtcctat aacgataatt ttcgcctcac aggaagcatt atgaaaaaga
     8881 tgatgatagc cacactggct gccgccagcg tgctgcttgc cgttgcaaat caggcgcatg
     8941 ctggagcgac gcttgatgcc gttcagaaaa aaggttttgt gcaatgcggg atcagtgatg
     9001 gattacctgg gttctcttat gccgatgcag acggtaagtt ttcaggtatt gatgttgatg
     9061 tttgtcgtgg tgttgccgct gctgtatttg gtgacgacac gaaagtgaaa tataccccac
     9121 tcacagcaaa agaacgcttc acggctttac agtcagggga agtggatttg ctctcccgta
     9181 atacgacctg gacttcatct cgcgacgccg ggatgggaat ggcatttact ggcgtcactt
     9241 attacgacgg cattggcttc ctgacgcacg ataaagcggg gctaaaaagc gcgaaagaac
     9301 tggatggcgc taccgtctgt attcaggcgg gtactgatac cgaactcaac gttgccgact
     9361 atttcaaggc aaacaatatg aagtacacac cggtgacttt cgatcgctct gacgaatcag
     9421 cgaaggcact ggaatctggt cgctgcgata cgctggcctc ggatcagtcg caactgtatg
     9481 ccctgcgcat caaattaagc aacccagctg aatggattgt cttaccggag gttatctcta
     9541 aagaaccgct tgggccggta gttcgtcgtg gcgatgatga atggttctcg attgtacgct
     9601 ggacgctttt cgccatgctg aatgctgaag agatgggcat caattcccag aacgtcgatg
     9661 aaaaagcggc taatccagca acgcctgata tggcacatct gctgggtaaa gaaggcgatt
     9721 acggcaagga tctgaagttg gataataaat gggcctataa catcatcaaa caggtgggta
     9781 actactcgga aattttcgag cgtaatgtag gttcagaaag cccgctgaaa attaaacgtg
     9841 ggcaaaataa tctctggaat aacggcggta ttcagtacgc gccgcccgtg cgttaagtcg
     9901 gtgtgaggta acgagcgtcg ctacggcggc gctctcttca gcgttatgct ttccgaggtt
     9961 tctatgtctc atcgccgctc aaccgttaaa ggctcactct cctttgccaa ccctacggtt
    10021 cgtgcctggt tattccagat ccttgccgtt gttgctgttg tcggcattgt tggttggtta
    10081 tttcacaaca ctgtaacgaa tctcagtaat cgtggcatta cttcaggttt tgcctttctg
    10141 gatcgcggcg ctggcttcgg tattgtccag catttgatcg attaccagca gggcgacacc
    10201 tacggacgcg tttttattgt cggcttactc aatacgctac tggtttctgc attgtgtatt
    10261 gtgttcgctt ctgttttggg cttctttatc ggtctggcga gactttcgga taactggctg
    10321 ctacgaaagc tttccacaat ttatattgag
//
LOCUS       DAZKGS010000149        10310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKGS010000149 DAZKGS010000000
VERSION     DAZKGS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            186..1580
                     /gene="galP"
                     /locus_tag="ACHOQY_003842"
     CDS             186..1580
                     /gene="galP"
                     /locus_tag="ACHOQY_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9679853.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1657..2154
                     /locus_tag="ACHOQY_003843"
     CDS             1657..2154
                     /locus_tag="ACHOQY_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9679854.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2249..2956
                     /gene="endA"
                     /locus_tag="ACHOQY_003844"
     CDS             2249..2956
                     /gene="endA"
                     /locus_tag="ACHOQY_003844"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9679855.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3036..3767
                     /gene="rsmE"
                     /locus_tag="ACHOQY_003845"
     CDS             3036..3767
                     /gene="rsmE"
                     /locus_tag="ACHOQY_003845"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9679856.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3780..4730
                     /gene="gshB"
                     /locus_tag="ACHOQY_003846"
     CDS             3780..4730
                     /gene="gshB"
                     /locus_tag="ACHOQY_003846"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9679857.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4839..5402
                     /locus_tag="ACHOQY_003847"
     CDS             4839..5402
                     /locus_tag="ACHOQY_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9679858.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5402..5818
                     /gene="ruvX"
                     /locus_tag="ACHOQY_003848"
     CDS             5402..5818
                     /gene="ruvX"
                     /locus_tag="ACHOQY_003848"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9679859.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5994..6974)
                     /locus_tag="ACHOQY_003849"
     CDS             complement(5994..6974)
                     /locus_tag="ACHOQY_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9679860.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            6992..7696
                     /gene="yggS"
                     /locus_tag="ACHOQY_003850"
     CDS             6992..7696
                     /gene="yggS"
                     /locus_tag="ACHOQY_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9679861.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7714..8280
                     /gene="yggT"
                     /locus_tag="ACHOQY_003851"
     CDS             7714..8280
                     /gene="yggT"
                     /locus_tag="ACHOQY_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9679862.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8277..8567
                     /gene="yggU"
                     /locus_tag="ACHOQY_003852"
     CDS             8277..8567
                     /gene="yggU"
                     /locus_tag="ACHOQY_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9679863.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8575..9168
                     /locus_tag="ACHOQY_003853"
     CDS             8575..9168
                     /locus_tag="ACHOQY_003853"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9679864.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9161..10297
                     /gene="hemW"
                     /locus_tag="ACHOQY_003854"
     CDS             9161..10297
                     /gene="hemW"
                     /locus_tag="ACHOQY_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9679865.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2425 a         2720 c         2722 g         2443 t
ORIGIN      
        1 gtcttaattg cagataacag cgtttaatct atgatgatat aactcaatta ttttcatgca
       61 cttaaaccat aactaagata aatgttagtg taagcgatta cactgatgtg atttgcttca
      121 catcttttta cgtcgtactc acctatctta attcacaata aaaaataacc atattggagg
      181 gcatcatgcc tgacgctaaa aaacaggggc ggtcaaacaa ggcaatgact tttttcgtct
      241 gcttccttgc cgctctggcg ggattactct ttggcctgga tatcggtgta attgctggcg
      301 cactgccgtt tattgcagat gaattccaga ttacttcgca cacgcaagaa tgggtcgtaa
      361 gctccatgat gttcggtgcg gcagtcggtg cggtgggcag cggctggctc tcctttaaac
      421 tcgggcgcaa aaagagcctg atgatcggcg caattttgtt tgttgccggt tcgctgttct
      481 ctgcggctgc gccaaacgtt gaagtactga ttctttcccg cgttctgctg gggctggcgg
      541 tgggtgtggc ctcttatacc gcaccactgt acctgtcaga aattgcgccg gaaaaaattc
      601 gcggcagtat gatctcgatg tatcagttga tgatcactat cgggatcctc ggtgcttatc
      661 tttctgatac cgccttcagc tacaccggtg catggcgctg gatgctgggt gtgattatca
      721 tcccggcaat tttgctgctg attggtgtct tcttcctgcc agacagccca cgttggtttg
      781 ccgccaagcg ccgttttgtt gatgccgaac gcgtgctgct acgcctgcgt gacaccagcg
      841 cggaagcgaa acgcgaactg gatgaaatcc gtgaaagttt gcaggttaaa cagagtggct
      901 gggcgctgtt taaagagaac agcaacttcc gccgcgcggt gttccttggc gtactgttgc
      961 aggtaatgca gcaattcacc gggatgaacg tcatcatgta ttacgcgccg aaaatcttcg
     1021 aactggcggg ttataccaac actaccgagc aaatgtgggg gaccgtgatt gtcggcctga
     1081 ccaacgtact tgccaccttt atcgcaatcg gccttgttga ccgctgggga cgtaaaccaa
     1141 cgctaacgct gggcttcctg gtgatggctg ctggcatggg cgtactcggt acaatgatgc
     1201 atatcggtat tcactctccg tcagcgcagt atttcgccat cgccatgctg ctgatgttta
     1261 ttgtcggttt tgccatgagt gccggtccgc tgatttgggt actgtgctcc gaaattcagc
     1321 cgctgaaagg ccgcgatttt ggcatcacct gctccaccgc caccaactgg attgccaaca
     1381 tgatcgttgg cgcaacgttc ctgaccatgc tcaacacgct gggtaacgcc aacaccttct
     1441 gggtgtatgc ggctctgaac gtactgttta tcctgctgac attgtggctg gtaccggaaa
     1501 ccaaacacgt ttcgctggaa catattgaac gtaatctgat gaaaggtcgt aaactgcgcg
     1561 aaatcggcgc tcacgattaa tctcctcagg cttcctccca tcgcggagga agccacctct
     1621 tgcagtcatc ttttcttcgc tctatcctct gccgctatga aaacatcccg tctccctatc
     1681 gccatccagc aggccgttat gcgccgcctg cgggaaaaac tcgcccaggc caacctgaag
     1741 ctagggcgta actacccgga gccaaaactc tcttacaccc agcgcggaac ctccgccgga
     1801 acagcctggc tggaaagcta tgaaatccgc ctcaatcccg ttttgctgtt ggaaaacagt
     1861 gaagctttta ttgaagaagt ggtaccgcac gaactggcac atttgctggt atggaaacat
     1921 ttcggccgcg tagcgccaca tggcaaagag tggaagtgga tgatggaaag cgtgctgggc
     1981 gttcccgccc gtcgtacgca tcagttcgaa ctgcaatccg tgcgtcgcaa caccttcccc
     2041 taccgctgca agtgccagga gcatcagctt accgtacgcc gccataatcg cgtagttcgt
     2101 ggcgaggccg tctatcgctg tgttcactgc ggtgaacagc tggttgcgaa ataaccatct
     2161 gaactatcag gaactttcct gatctggctg attgcatacc aaaacaactt tcgctacgtt
     2221 gctggctcgt tttaacacgg agtaagtgat gtaccgttat ttgtctattg ctgcggtggt
     2281 actgagcgca gcattttccg gcccggcgtt ggccgaaggt atcaatagtt tttctcaggc
     2341 gaaagccgcg gcggtaaaag tccacgctga cgcgcccggt acgttttatt gcggatgtaa
     2401 aattaactgg cagggcaaaa aaggcgttgt tgatctgcaa tcgtgcggct atcaggtgcg
     2461 caaaaatgaa aaccgagcca gccgcgtaga gtgggaacac gtcgttcccg cctggcagtt
     2521 cggtcaccag cgccagtgct ggcaggacgg tggacgtaaa aactgcgcta aagatccggt
     2581 ctatcgcaag atggaaagcg atatgcataa cctgcaaccg tcagtcggcg aggtgaatgg
     2641 cgatcgcggc aactttatgt acagccagtg gaatggcggc gaaggccagt acggtcaatg
     2701 cgccatgaag gtcgatttca aagaaaaagc tgccgaacca ccagcgcgtg cacgcggtgc
     2761 cattgcacgc acctacttct atatgcgcga ccaatacaac ctgacactct ctcgccagca
     2821 aacgcagctg ttcaacgcat ggaacaagat gtatccggtt accgactggg agtgcgagcg
     2881 cgatgaacgc atcgcgaagg tgcaaggcaa tcataatccg tatgtgcaac gcgcttgcca
     2941 ggcgcgaaag agctaaccta cactagcggg actctttttg ttaaccccta ccccacgcgt
     3001 acaaccgcgt ggggagacga cgcggatttt taactatgcg tatcccccgc atttatcatc
     3061 ctgaaccact gaccagccat tctcacatcg cgctttgcga agatgccgcc aaccatatcg
     3121 ggcgcgtact gcgcatgggg ccggggcagg cgttgcaatt gtttgacggt agcaaccagg
     3181 tctttgacgc cgaaattacc agcgccagca aaaaaagcgt ggaagtgaag gtgctggaag
     3241 gcaagatcga cgatcgcgaa tctccgctgc atattcacct cggtcaggtg atgtcgcgtg
     3301 gtgaaaaaat ggaatttacg atccagaaat cgatcgaact cggtgtaagc ctcattacgc
     3361 cacttttttc tgagcgctgc ggcgttaaac tggatagtga acgtctgaac aagaagctcc
     3421 agcagtggca gaagattgca attgctgcct gtgagcagtg tggtcgtaac cgggtgccgg
     3481 aaatccgccc agcgatggat ctggaagcct ggtgtgcaga gcaggatgaa ggactgaaac
     3541 tgaatcttca cccgcgcgcc agtaacagca tcaatacgtt gccgttaccg gttgaacgcg
     3601 tccgcctgct gattggcccg gaaggcggtt tatcggcaga tgaaattgcc atgactgccc
     3661 gctatcaatt tactgatatc ctgttgggac ctcgcgtttt gcgtacagag acaactgcgc
     3721 tcaccgccat taccgcgcta caagtacgat ttggcgattt gggctaacgg agaagaataa
     3781 tgatcaagct cggcatcgtg atggacccca tcgcaaacat caacatcaag aaagattcca
     3841 gtttcgctat gttgctggaa gcacagcgtc gtggttacga acttcactat atggagatgg
     3901 gcgatctgta tctgatcaat ggcgaagccc gcgcccatac ccgcacgctg aacgtgaagc
     3961 agaactacga agagtggttt tcgttcgtcg gtgaacagga tctgccgctg gccgatctcg
     4021 atgtgatcct gatgcgtaaa gacccgccgt ttgataccga gtttatctac gcgacctata
     4081 ttctggaacg tgccgaagag aaagggacgc tgatcgttaa caagccgcag agcctgcgcg
     4141 actgtaacga gaaactgttt accgcctggt tctctgactt aacgccagaa acgctggtta
     4201 cgcgcaataa agcgcagcta aaagcgttct gggagaaaca cagcgacatc attcttaagc
     4261 cgctggacgg tatgggcggc gcgtcgattt tccgcgtgaa agaaggcgat ccaaacctcg
     4321 gcgtgattgc cgaaaccctg actgagcatg gcactcgcta ctgcatggcg caaaattacc
     4381 tgccagccat taaagatggc gacaaacgcg tgctggtggt ggatggcgag ccggtaccgt
     4441 actgcctggc gcgtattccg caggggggcg aaacccgtgg caatctggct gccggtggtc
     4501 gcggtgaacc tcgtccgctg acggaaagtg actggaaaat cgcccgtcag atcgggccga
     4561 cgttgaaaga aaaagggctg atttttgttg gtctggatat catcggcgac cgtctgactg
     4621 aaattaacgt caccagccca acctgtattc gtgagattga agcagagttt ccggtgtcga
     4681 tcaccggaat gttaatggat gccatcgaag cacgtttaca gcagcagtaa cccaccttag
     4741 cgagaaggat ctcgttgaga ctctgagtga cagcgccctt ctttccacgc atactgggcg
     4801 ctgttgcttt tttgaaccag gaaacagaac ctctgacaat gaatttacag catcactttc
     4861 ttattgccat gcctgctctc caggatccga ttttccgtcg ttccgtggtc tacatttgcg
     4921 aacataatac caatggtgca atggggatca tcgtcaacaa gccgctggaa aatctcaaaa
     4981 ttgaagggat tctggaaaag ctgaagatca cgccggagcc gcgtgatgaa tcaatccgtc
     5041 tggataaacc ggttatgctc ggcggtccgc tggctgaaga tcgcgggttt attttgcata
     5101 ctccgccctc caattttgct tccagcattc gcatttcaga caacacagtg atgaccactt
     5161 cccgcgatgt gctggaaacg ctcggcaccg ataaacaacc gtctgacgta ttggtggctc
     5221 tgggttatgc ctcctgggaa aaaggtcaac tggaacaaga aattctcgat aacgcgtggc
     5281 taacggcccc ggcggatctg aatattctgt tcaaaacgcc gattgccgac cgctggcgcg
     5341 aggcggcaaa actgattggt gtggatattc tcaccatgcc tggtgtggca gggcacgcct
     5401 gatgagtgga accttactcg ccttcgactt cggcaccaaa agcattggcg tagcggtcgg
     5461 ccaacgcatt accggcactg ctcgcccttt gcctgcaatt aaagcacagg acggtacgcc
     5521 ggactggaac ctcatcgaac gtttactgaa agagtggcag ccggacgaaa tcatcgtcgg
     5581 tttgccgctg aatatggacg gcaccgagca accattgact gccagagcgc gtaaatttgc
     5641 caaccgtatt catggtcgtt tcggtgttga agtaaagctc catgacgaac gtcttagcac
     5701 cgtggaagcc cgttccggtc tgtttgaaca gggcggctat cgggcgctca acaaaggcaa
     5761 agtcgactct gcctctgcgg ttattattct cgaaagctat ttcgagcagg gatattaagg
     5821 cgatctaaaa gcctggcgga tagtcgttac cgccatcttg ccggatgcgg cgtgaacgcc
     5881 ttatccagca cacatctggc agcggctgta ggtctgataa gacgcgttag cgtcgcatca
     5941 gacatttatt gcctttatag gccctataaa cttgcgcatc gggcatggca acgtcacaaa
     6001 cgcccttccc ccacccgctg ctgataactc tgctgaaacg ttaacatccc cacctgctgc
     6061 ccggtttgaa taacatgcgg taactggtgg gttttccctt cgcgaatcaa attccccacc
     6121 gcgggagtgt taatcaataa ttcaaacagc gccacgcgtc cttcctgttt atccacttcc
     6181 agcttttgtg acaacacggc ccgtaaacta cctgccagtt gattacgtac gggatctttt
     6241 tcctgcgccg gaaatgaatc caccagtcgc tcaactgcct gcgcggcacc acgcgtatgt
     6301 aatgttgcca gcaccaaatg cccggtttct gccgccgtca gtgccagacg gattgtctcg
     6361 ctgtcacgca gctctccgag caaaatcaca tcaggatctt cccgcaatgc ggcccgcaat
     6421 cccgatgcga acgtcataca gtgcaaacca atttcccgct gctggatcaa acatcgctgg
     6481 ctggcataga tatattcaac aggatcttcc agcgtcagaa tatgcgcatc ggcatgttga
     6541 tagagatagc caaccatcgc cgctagcgtg gtagatttgc cgctccccgt cgcccccgtc
     6601 accagaatca ggccattctc gctcttgagt aattccggca ataccgttgg tgcgccaagc
     6661 tgttcgagct gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat accttgccgt
     6721 tgcgcgaacg cactgccacg caaccgctgg ttttccgcca gcgacacggc aaaatccagc
     6781 tggccatttt ccagcaatat tgcccgctga tcgtcatcca gccactcccg cagtagctct
     6841 tcgacgtccg gcgcatcaaa cggcgcagct tccattagcc cgcgaatgcg ccatcgtgcg
     6901 gaccaggcgc tgcacaggtg tagatccgag acgttatgct ttacactaag ggccacaatt
     6961 tcttccatat tcatactaag atcctcggaa aatgaacgat attgcgcata acctggcaca
     7021 ggtccgggac aaaatctcag cggctgcaac gcgttgcggc cgttctccag aagaaattac
     7081 gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca ttgatgccgg
     7141 gcagcgtcaa tttggtgaaa actacgttca ggaaggggta gataaaattc gccactttca
     7201 ggaactgggc gtaacaggat tagaatggca ttttattggc ccgttgcagt ctaataaaag
     7261 ccgcctggtg gcagagcatt tcgactggtg tcataccatc gaccgtttgc gcatcgctac
     7321 ccgcctcaac gatcagcgcc cggcagaact tccccctctt aacgtactga ttcaaattaa
     7381 tattagtgat gaaaacagta agtccgggat tcaactggca gaactggacg agctggcagc
     7441 tgcggtcgct gaactaccgc gtttacgtct gcgcgggctg atggcaatcc ctgcacctga
     7501 gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat ttgccggact
     7561 gaaaacgcgc tacccgcata tcgacacgct ctctctggga atgtcggacg atatggaagc
     7621 cgccattgcg gcaggtagca cgatggttcg tatcggcact gcaatttttg gtgcgcgtga
     7681 ttactctaaa aaataaggaa ttaaaggaac gccatgaata cgttgacttt cctgctttca
     7741 acggtcattg agctgtatac catggtgctg ttattacgca tctggatgca gtgggctcat
     7801 tgtgattttt acaacccctt ctcacagttt gtagtgaagg taacgcagcc aattatcggg
     7861 ccactgcgcc gcgttattcc ggcaatgggc cccattgaca gcgcctcgct gctggttgcc
     7921 tatattctca gttttatcaa agccatcgtg ctgtttaaag tggtgacctt cctgccaatc
     7981 atctggattg ccggtttact gattctgctg aaaaccatcg gcctgctgat tttctgggtc
     8041 ctgctggtga tggcgattat gagctgggta agccagggtc gtagcccgat tgaatacgtg
     8101 ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc gcctgctacc ggcaatgggt
     8161 gggattgatt tctcgccgat gatcctcgtt ctgctgctgt atgtcatcaa tatgggtgtc
     8221 gcagaagtat tacaggcaac cggaaacatg ctgctgccgg ggctgtggat ggcgttatga
     8281 gtgccgtaac agttaatgat gacggtctgg ttttacggct ctatattcag ccgaaagcca
     8341 gccgtgattc tattgtcggt ttacatggcg acgaagttaa agtcgccatt accgcgccgc
     8401 cggttgacgg tcaggccaac agtcatctgg tgaagtttct cggtaagcaa ttccgggttg
     8461 ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa attaaaatca
     8521 ttaatccgca acaaatcccg ccagaaatcg cggcgttaat taattaggta tcctatgcaa
     8581 aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc gctgcttagc
     8641 gacttcggtc ttgatatcgt ggcccaaaca gacctcggcg tagattccgc tgaagaaacc
     8701 ggcctgacct ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa agtgaccggt
     8761 ttaccggcaa ttgccgacga ctctggtctg gcggtagatg tgcttggcgg cgcgccgggc
     8821 atttactccg cgcgttattc cggtgaagac gcgaccgatc taaagaatct gcaaaaactg
     8881 ctggaaacac tgaaagacgt accggatgac caacgtcagg cgcgtttcca ctgcgtgctg
     8941 gtatatctgc gtcacgcgga agatccgact ccgctggtat gccacggtag ctggccgggc
     9001 gtgattactc gtgaaccagc gggcactggt ggctttggtt atgatccaat cttcttcgta
     9061 ccttccgaag ggaaaaccgc cgccgaactg acccacgaag aaaagagcgc catttcccac
     9121 cgtggtcagg cattgaaact gctgctggac gctttacgta atggttaaat taccgccgct
     9181 gagtctctac attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg atttcaactc
     9241 tcacgcgttg aaaggagaag tgccgcacga cgattacgtt cagcatctgc tttgcgatct
     9301 ggacaacgat gtggcttacg ctcagggccg tgaagtaaag acaatcttta ttggcggtgg
     9361 tacgccgagc ctgctttccg gcccggcgat gcaaacgctg ctggacggcg tgcgtgcgcg
     9421 tttgccgctg gcagcggatg cagaaattac tatggaagcg aaccctggta cggtagaagc
     9481 cgatcgcttt gtcgattatc agcgtgctgg tgtgaaccgc atctctattg gtgtgcagag
     9541 ttttagcgaa gacaagctga aacgacttgg gcgtattcat ggcccgcaag aagcgaaacg
     9601 cgcggcaaag ctggcgagcg ggctggggct gcgtagcttt aacctcgatt tgatgcatgg
     9661 actaccggat caatcactgg aagaggcgct tggcgatcta cgccaggcca ttgaactgaa
     9721 tccgccgcat ctttcctggt atcaactgac catcgaacct aatacgctgt ttggttcacg
     9781 cccgccggta ctgccggacg acgacgcgct atgggatata ttcgaacagg ggcatcagtt
     9841 attaaccgca gcgggttatc agcaatatga aacttccgct tacgccaaat ccagttatca
     9901 gtgccagcac aatctcaact actggcgctt tggcgactac atcggtattg gctgcggcgc
     9961 gcacggcaaa gtgaccttcc cggatgggcg cattctgcgt accactaaaa cgcgtcatcc
    10021 gcgtggtttt atgcaaggaa ggtatctgga aagccagcgt gatgtcgaag ccgcagataa
    10081 gccgtttgag ttctttatga atcgcttccg tctgctggag gccgcgccgc gcgtggagtt
    10141 tattgcgtat accgggcttt gcgaagatgt gattcgccca cagttagacg aggcgattgc
    10201 ccagggttat ctcaccgaat gtgcggatta ctggcagata acggaacatg gtaaattatt
    10261 cttaaactca cttcttgaat tattcctcgc tgagtaatca ttaatgcgtc
//
LOCUS       DAZKGS010000150        10303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKGS010000150 DAZKGS010000000
VERSION     DAZKGS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..5319
                     /locus_tag="ACHOQY_003855"
                     /pseudo
     CDS             144..5319
                     /locus_tag="ACHOQY_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5542..5808
                     /gene="hipB"
                     /locus_tag="ACHOQY_003856"
     CDS             5542..5808
                     /gene="hipB"
                     /locus_tag="ACHOQY_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP9679866.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5808..7130
                     /gene="hipA"
                     /locus_tag="ACHOQY_003857"
     CDS             5808..7130
                     /gene="hipA"
                     /locus_tag="ACHOQY_003857"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP9679867.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7442..7650
                     /locus_tag="ACHOQY_003858"
                     /pseudo
     CDS             7442..7650
                     /locus_tag="ACHOQY_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8082..8645
                     /gene="fimA"
                     /locus_tag="ACHOQY_003859"
     CDS             8082..8645
                     /gene="fimA"
                     /locus_tag="ACHOQY_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9679868.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            9006..9716
                     /locus_tag="ACHOQY_003860"
     CDS             9006..9716
                     /locus_tag="ACHOQY_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9679869.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9758..>10303
                     /locus_tag="ACHOQY_003861"
     CDS             9758..>10303
                     /locus_tag="ACHOQY_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFP9679870.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEE"
BASE COUNT         2861 a         2199 c         2645 g         2598 t
ORIGIN      
        1 gtattagcaa attagccaaa ttgacacctt cccacctcca actaacactc aatacagacc
       61 tctgacaatt taataagttt catttttaat gtctgacgac tcatctcttt cgtggctgta
      121 ttttttgtat atggagcgtt tatatgaata gaatctatcg cgtgatatgg aattgcactc
      181 tacaggtatt tcaggcctgc tcggaattaa ctcgcagggc aggtaaaaca tcgacggtta
      241 atttgcgtaa atcctctgga ctgacaacga aattcagtag attgacgctg ggtgttttgc
      301 tggcactaag cggttcagcg tctggtgcaa gtctggaagt tgataatgat cagattacca
      361 atattgatac tgatgttgct tatgatgcct acctggttgg ctggtatggc actggagtgc
      421 ttaatatttt ggctggcggt aatgcctcct taaccactat tactaccagc gtcattggcg
      481 ctaatgagga ctcagagggc accgttaatg ttttgggtgg cacctggcga ttgtatgata
      541 gcggaaataa tgcaaggcct ttaaatgtgg gtcaatccgg aacggggacg ctgaatatta
      601 aacagaaggg tcacgtcgat ggaggctatt taagattagg ttcttcgaca ggaggcgtcg
      661 ggacggtcaa tgttgaggga gaggactctg ttctgacgac cgaattattc gaaataggga
      721 gttacggtac gggctcatta aatattacgg ataagggtta cgtcacgagt tcaatcgtcg
      781 ccattttagg ctatcaggcg ggcagtaatg gccaggttgt cgttgaaaag ggtggcgagt
      841 ggctaataaa aaataacgat tcctcaattg aatttcaaat tggtaatcaa ggaactgggg
      901 aggcgactat tcgcgagggt ggactgatta cggctgaaaa tacgattatc ggtggcaatg
      961 ccaccggtgt cggaaccctg aatgtgcagg atcaagactc tgtcatcacg gtacgcagac
     1021 tctataatgg atatttcggt aatggcacac tcaatatttc caataatgga ctaattaata
     1081 acaaagaata ttcattagtg ggcgttcagg acggttccca cggtgtcgtc aacgtgaccg
     1141 ataaagggca ttggaatttc ctcggaacgg gcgaagcttt ccgctatatc tatatcggtg
     1201 atgctggcga cggtgaactt aatgtctcgc gtgaaggtaa agtagattcg ggaattatca
     1261 ctgcggggat gaaagaaaca ggcacaggca acattactgt taaggataag aactccgtta
     1321 tcactaatct cggaactaat cttggttatg acggccacgg cgaaatgaat atcagtaatg
     1381 aagggcttgt tgtcagcaac ggaggaagct cactcggtta tggagaaacc ggcgtcggga
     1441 atgtcagcat caccacgggg gggatgtggg aggtcaataa gaatgtctat accaccattg
     1501 gtgttgcggg cgtcggaaac ctcaatatta gcgatggcgg taagttcgta tcgcaaaata
     1561 ttactttttt gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgcgacat
     1621 catcgttcga tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca
     1681 gtaatggtgc cacccttaat tcaacgggct atggatttat cggaggaaat gcctccggta
     1741 aggggatagt taatatttca acggacagtc tctggaattt aaagacatca tctactaacg
     1801 cacaattgct acaggtcggt gtattaggca agggtgaact gaatattacc accggaggta
     1861 tagttaaagc gcgtgataca cagatagctc tcaatgacaa aagtaagggc gacgtgaggg
     1921 tggatgggca gaactctctt cttgaaacat tcaatatgta cgtagggaca tctggtacgg
     1981 gtacgttaac cctgacgaat agcggcacgc tgaatgtcga aggtggagaa gtttacttag
     2041 gtgtttttga acctgctgta ggaacgctaa acattggtgc agctcacggt gaggcggcgg
     2101 cagatgccgg gtatataacc aacgcgacga aagtagagtt tggttctggc gaaggtgttt
     2161 ttgtctttaa tcatactaat aacagtgatg ccggctacca ggtcgatatg ctgattacag
     2221 gtgacgataa agacggaaaa gtgatccatg atgcaggcca tacggtgttc aatgcaggga
     2281 atacttatag cggtaaaacg ctggtcaatg acggcctcct gaccattgcg tctcatacgg
     2341 cagatggggt aacgggcatg ggttcgagtg aagtcaccat tgcaagcccc ggtacgctcg
     2401 acattctcgc atcaacgaac agtgcaggag attacacgct gaccaatgcg ctcaaaggcg
     2461 atggcttgat gcgagtgcag ctgtcatcct acgacaagat gtttggcttt acccatgcaa
     2521 cagggactga attcgccggt gttgcccaac tgaaagacag taccttcact ctggaacgcg
     2581 acaacaccgc tgcgcttact cacgcgatgt tgcagtctga cagtgaaaat accacatcgg
     2641 taaaagtggg agagcaatcc attggtggac tggccatgaa tggcggtacg ctcattttcg
     2701 atacggatat tcctgctgcg acgcttgcag agggatatat cagcgtcgat acgctggttg
     2761 tcggcgcggg tgactacacc tggaaaggcc gtaactatca ggtaaacggg acgggcgacg
     2821 tgcctaaacc gtggaatgat cccatggcga ataaccctct gacgacgctc aatttgctgg
     2881 aacacgatga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt attgggtcgg
     2941 gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac aaaacgttac
     3001 acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc ctcacgaccg
     3061 caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc catggtgggc
     3121 aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat atgtcggcaa
     3181 aaatcggtgg tgaaggggat ctggcaatca atacggtgcg acaggtttcg ctttccaacg
     3241 gtcagaacga ctatcagggg gcaacctacg ttcagatggg gacattacgt accgatgcgg
     3301 atggtgcact tggcaacacc cgggaactga acatcagcaa cgcggccatc gtcgatctta
     3361 atggatcgac gcagacggta gagacattca ccgggcagat gggttcgact gttttgttca
     3421 aagagggggc gctgacggta aataaaggtg ggatcagtca gggtgaactg acaggtggcg
     3481 gaaacctgaa tgttacaggg ggaacgctgg ctatcgaggg gcttaatgca cgctacaatg
     3541 cgttaaccag cattagccca aatgcggaag tcagcctcga taatacgcag gggttaggca
     3601 gaggaaatat tgccaataac ggtctgttaa cgctaaaaaa cgtgactggc gaactgcgca
     3661 atagcataag cgggaagggt atcgtgagcg caaccgccag gacagatgta gagctggatg
     3721 gcgataatag ccgctttgtg gggcaattca acattgatac aggcagcgcg ctcagcgtca
     3781 acgagcagaa aaacctgggt gatgcttccg ttatcaataa tggcctgctc accatctcca
     3841 ctgagcgtag ctgggcgatg acgcacagta tcagcggtag cggtgatgtg acaaaactgg
     3901 gtaccgggat cctgactctt aacaacgatt ccgcggcgta tcagggtacg acggatatcg
     3961 tgggggggga aattgctttc ggttccgact ctgccattaa tatggcaagt caacacatta
     4021 atatccataa cagcggtgtg atgtcgggaa atgtcaccac tgcaggtgat gtgaacgtta
     4081 tgcctggggg gacactgcgt gtcgctaaaa ccacaatcgg cggcaacctg gagaatggcg
     4141 gcacggttca aatgaatagc gaagggggga aaccggggaa tgtactgacc gttaacggca
     4201 actataccgg aaacaatggc ctgatgacgt tcaacgcgac gctgggcggc gataattcgc
     4261 ccaccgataa aatgaacgtg aaaggcgata cccaagggaa cactcgcgtt cgggttgata
     4321 acattggcgg cgtcggtgca caaacggtca acggtattga actcattgag gttggcggta
     4381 attctgcagg taacttcgcg ctgaccaccg gaactgtcga agctggggct tacgtctaca
     4441 cgctggctaa agggaagggg aatgacgaga aaaactggta tctgaccagt aaatgggacg
     4501 gcgtaacgcc agcggataca cccgatccca tcaataatcc ccctgttgtg gatccggaag
     4561 gcccatcagt ttatcgcccg gaggccggaa gctatatcag caacattgcc gcagccaact
     4621 cgctgtttag ccatcgttta cacgaccgtc tgggtgagcc gcagtatata gattcactgc
     4681 attctcaggg gtcggcaagc agtatgtgga tgcgtcatgt cggagggcac gaacgttcaa
     4741 gggccggtga cggtcagcta attactcagg ctaaccgcta tgtattgcag ctaggcggcg
     4801 atttggcgca gtggagtagc aacgcgcagg atcgctggca tgacggcctc tgttgagggt
     4861 gggtatacct ttgaagcggg aacatttaga ggcagcgaag ggacgctgaa tacctggtac
     4921 gtccagccac aggcgcaaat cacctggatg ggtgtgaaag attctgacca taatcggaaa
     4981 gacggaacac gcattgaaac ggaaggcgac ggaaacgtgc aaacgcgact tggggtgaaa
     5041 acctacctga atagccatca caagcgtgac gatggtaaac agcgtgagtt ccagccttac
     5101 attgaagcga actggatcaa caatagcaaa gtctacgccg tgaagatgaa tggtcaaacc
     5161 gtaagccgtg atggtgcgcg taatctcggt gaagtacgta ccggggttga ggcgaaagta
     5221 aataacaacc ttagcctgtg ggggaatgtc ggtgtgcaac taggtgataa aggctatagc
     5281 gatactcagg gcatgctggg agtgaaatat agctggtaaa ccgtataagc cgcatgtcga
     5341 gatggcatgc ggcttaatat tgccgacttc aaacggcgca tcagcgcctt ttttaaatcc
     5401 tcctttttat ccgcgatcgc ggatatcgca gcgtttatcc cgtagagcgg ataagacgtg
     5461 tttccaaatt gacttatcct cactaaagga taaaactgat aatatcctct tatgcggata
     5521 aatttgctgt ggacgtatga catgatgagc tttcagaaga tctatagccc aacgcaattg
     5581 gcgaatgcaa tgaaactggt tcgccagcaa aatggctgga cgcagagcga gctggcgaaa
     5641 aaaattggca ttaagcaggc gacaatttcc aatttcgaaa acaaccctga caataccacg
     5701 ctcacgacat tttttaagat tttacagtcg cttgaactct caatgacact atgcgacacg
     5761 aaaaatgcct cgccagaatc aacagaacag caagatctgg agtggtaatg cctaaacttg
     5821 tcacttggat gaacaaccag cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca
     5881 cctttaagta tgcaccggag tggttagcca gccgttatgc cagaccgttg tcactttcgc
     5941 tgccattgca gagggggaat atcacctcag atgccgtatt taacttcttc gataacctgt
     6001 tacccgatag cccgattgta cgtgaccgga tcgttaaacg ttatcatgct aaatccagac
     6061 aaccgtttga tttattgtca gaaatagggc gagacagcgt tggtgccgta acgttattac
     6121 ccgaaaatga aaccataacg cgcccgataa tggcatggga aaagcttact gaagccaggc
     6181 ttgaagaagt attaacggct tataaagcag atatccctct gggcatgatt agagaagaaa
     6241 atgactttcg catctcggtt gctggcgcgc aggagaagac agcgctgctt agaataggca
     6301 atgactggtg cattccgaaa ggaataacgc cgacgacgca catcattaaa ttaccgattg
     6361 gcgaaatcag acagcccaat gcgacgctcg atctcagcca aagcgttgat aatgagtatt
     6421 actgtctgct gctggcgaaa gaacttgggt tgaatgttcc ggacgcagaa atcattaaag
     6481 cgggaaatgt gcgcgcgtta gcggtcgaac gttttgacag gcgttggaat actgagcgaa
     6541 cggttttact tcgcttgcca caggaggata tgtgtcagac attcggttta ccttcatctg
     6601 tgaaatatga atcagatgga ggcccaggca tcgcgcggat catggcgttt ttgatggggt
     6661 ccagcgaggc gcttcgcgat cgttatgatt ttatgaaatt ccaggtcttc cagtggttga
     6721 ttggcgcaac ggatggccat gcaaaaaact tctccgtatt tattcaggct ggcggcagtt
     6781 atcgactcac gccattttac gacatcattt cagcatttcc ggtccttggc ggtacgggaa
     6841 tacacatcag cgatctcaaa ctggcaatgg ggcttaacgc atccaaaggc aaaaaaacgg
     6901 caatcgataa aatttatccg cgacattttt tggcgacagc aaaggtgctg agattcccgg
     6961 aagtgcagat gcatgaaatc ctaagtgact ttgccagaat gattccggca gcactggata
     7021 acgtgaagac ttcattaccg acagattttc cggagaacgt ggtgacggca gttgaaacca
     7081 atgtgttgag gttgcatgga cggttaagcc gagaatacgg tagtaagtga gatatggggt
     7141 ttggtcgttg ttaatgagca cgacaatcat gaccgccaac gactggattc ttccttggtt
     7201 tatactggtg cgcataatgt atattatgtt aaattatgtc tgagtgatgc gaagtgcttt
     7261 tctggtagtc gttattcgtt cagggttcaa gaacttaagc ctctcctctt tcataattgg
     7321 cctgtgcatg gagaaatagc ctggttattc cctgccccat gctaagagcg cagtgtctgt
     7381 cgcatagaca ctgcatacga aaacaccaga gggtgctgtt acttatttaa ggacggatca
     7441 agcgggaatg agtccactcc ggtatcagaa atggctacgt ctcaatgaag tcaggcgacc
     7501 gatgctgaat gaacattacg atgtcaccac agcagcctat gctgtcggtt acgaaagcta
     7561 tcccatttca gtcgggaata ttcgcggatg tttggagagt cacccaagag agatattacc
     7621 aggttaagaa agtctgtcgg tcagctttaa taaaccctgc acttatctgt ttagagcaat
     7681 tcggtgttag tttcagcaag caaacattaa ccatagctaa tgatttatag ccatattaac
     7741 cattggtagc ccttgacctt tcctgacact tcccttgttc gcaataatat tctgtttagt
     7801 cacggtttat aaataacatg cataatgaaa tcgtcatgtt atttagcgac ataaaatgtt
     7861 tttgcaaata ctctcttcaa caagtgcaca ataaacttgt tgtgattagg tgtccttgtg
     7921 atttaaagat attatcgttc ttcgtgaaat gtaataaccc tggaaactat attctcaggg
     7981 caacaaccaa tcaagaatga ttcttgtttt taagcaataa cagaatgact taattgttaa
     8041 ccctctttat tcagctactt aaatataaat tttggagaat tatgaaactc aaacatgttg
     8101 gtatgattgt cgtttctgtg ttggcgatgt cgtctgctgc ggtaagcgca gccgagggtg
     8161 atgaatcagt aacgaccact gttaatggcg gtgttattca ttttaaaggt gaagtggtaa
     8221 atgccgcttg tgcaattgat tcagaatcaa tgaaccaaac ggttgagctg ggtcaggttc
     8281 gttcttctcg cttggctaaa gcgggtgacc tcagctccgc cgttggcttc aatatcaagc
     8341 tgaatgattg tgataccaat gtttccagta atgcagctgt tgcattcctg ggtactactg
     8401 tcaccagtaa tgacgatacg ttagcgctgc agagttcagc ggcaggctct gcccaaaatg
     8461 tcggtattca aattttggac cgtacgggtg aggtattaat acttgatggg gccactttta
     8521 gtgctaaaac cgacttgatt gatggcacga atatactgcc attccaggct cgttatattg
     8581 ctctcgggca gtccgtagct ggtactgcaa acgctgatgc gaccttcaaa gttcaatatc
     8641 tataattcaa tcttaaggtg gctatacgtc atctgaaatt gaaacagggt gtgtgtggca
     8701 tggctacgca cacccgagca atttaccaat attcatctca tttttaatga gaaatggttt
     8761 gtatttggag cgctatgaaa aatcacataa tatctaaact atcaataaac aacagtagtt
     8821 aaacttactg ttatttaaaa tatttacttt gtagcaaggt tttcctatta aaagagaacc
     8881 aacatttatt acaaacgcat taagtctgga aacccggcga ctcgccaatg tattagcaac
     8941 attaatcgat tgtctgatgt aacggttttc ccgcttatcc tcttaacaca tggagaatgc
     9001 atcagatgca aactacgcgt accccctact ctatttcctt tatggcgaca gtgttacttt
     9061 tattgctctt tgcttgccat agcaccgttg ccaacgcagc tgtcgcactg ggtgcgacgc
     9121 gggtaattta tccggcaaac cagaaacagg ttttattgcc tgtcacgaat aatgatcctg
     9181 ccagtgtcta tctcattcag tcatggatag aaaatgcagg tgaccaaaaa gacacccagt
     9241 ttgtcattac cccaccgctt ttttccatgc aaggtaaaaa agagaatacg ctgcggataa
     9301 tcaacgctac taatcatcag ttaccaggcg accgtgaaag cctgttctgg gtgaatgtta
     9361 aagctattcc tgctatggaa aaagaccaga agaatgagaa tacattacag ctggcaatta
     9421 ttagccgcat aaaaatgttt tatcgcccca cgaacctggc aatggcacct gaagaagcgc
     9481 ctgcaatgct tcggtttcgt cgcagcggta gcaaactcac gcttattaat ccaacgccct
     9541 atttcattac cgtaacaaat atgaaagcag gaaatagcaa tctcccaaac accatggtgc
     9601 cgccaaaagg tgaagtatcg gtggatatca cgcatgctgc aaccggggat atcagttttc
     9661 agacgatcaa tgattacggt gcattaactc cgcgtattaa ggcaaccatg cagtaagcca
     9721 gtgacctaat taataataaa ctcaatcgga attaataatg acagcctttc gtgccgcatt
     9781 taaggcatat cgtatgcatc aggtgttaat aatgcctcgc tttgcacggc ttaccattgc
     9841 ccttggctta gcaactgcgg tatttcccgt tgacgcagag tattatttta atccacggtt
     9901 tttaagcaat gatcttgctg aatctgtcga tttatccgcc tttactaagg gacgcgaggc
     9961 accacctggt acatatcggg tcgacattta tttgaacgac gaatttatga ctagtcggga
    10021 tattactttt attgcagatg ataataacgc agaactcatc ccatgcctga gtacagatct
    10081 tcttgtcagt cttggtataa aaaaaatagc gttattagac aataaagaac attcggcaga
    10141 aaaacatgtg ccggacaaca gcgcatgcac accacttcag gatcgtttgg ccgacgcatc
    10201 aactgagttt gatgttggtc agcaacattt gtcactctcc gttccgcaaa tttatgttgg
    10261 cagaatggct cgcggctatg tttctcctga tctgtgggaa gaa
//
LOCUS       DAZKGS010000151        10275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKGS010000151 DAZKGS010000000
VERSION     DAZKGS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1172
                     /gene="ybaT"
                     /locus_tag="ACHOQY_003862"
     CDS             <1..1172
                     /gene="ybaT"
                     /locus_tag="ACHOQY_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9679871.1"
                     /translation="MEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGL
                     GNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMT
                     LFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGI
                     TFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEK
                     YADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLM
                     NKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIH
                     ASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            1203..1300
                     /locus_tag="ACHOQY_003863"
                     /pseudo
     CDS             1203..1300
                     /locus_tag="ACHOQY_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1297..1704
                     /gene="cueR"
                     /locus_tag="ACHOQY_003864"
     CDS             1297..1704
                     /gene="cueR"
                     /locus_tag="ACHOQY_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9679872.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1705..2163)
                     /gene="ybbJ"
                     /locus_tag="ACHOQY_003865"
     CDS             complement(1705..2163)
                     /gene="ybbJ"
                     /locus_tag="ACHOQY_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9679873.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITSL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2160..3077)
                     /gene="qmcA"
                     /locus_tag="ACHOQY_003866"
     CDS             complement(2160..3077)
                     /gene="qmcA"
                     /locus_tag="ACHOQY_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9679874.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3223..3900
                     /gene="fetA"
                     /locus_tag="ACHOQY_003867"
     CDS             3223..3900
                     /gene="fetA"
                     /locus_tag="ACHOQY_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9679875.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3887..4666
                     /gene="fetB"
                     /locus_tag="ACHOQY_003868"
     CDS             3887..4666
                     /gene="fetB"
                     /locus_tag="ACHOQY_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9679876.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4729..5583)
                     /gene="cnoX"
                     /locus_tag="ACHOQY_003869"
     CDS             complement(4729..5583)
                     /gene="cnoX"
                     /locus_tag="ACHOQY_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9679877.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5644..6453)
                     /gene="ybbO"
                     /locus_tag="ACHOQY_003870"
     CDS             complement(5644..6453)
                     /gene="ybbO"
                     /locus_tag="ACHOQY_003870"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9679878.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6443..7066)
                     /gene="tesA"
                     /locus_tag="ACHOQY_003871"
     CDS             complement(6443..7066)
                     /gene="tesA"
                     /locus_tag="ACHOQY_003871"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9679879.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7037..7723
                     /gene="ybbA"
                     /locus_tag="ACHOQY_003872"
     CDS             7037..7723
                     /gene="ybbA"
                     /locus_tag="ACHOQY_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9679880.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7720..10134
                     /gene="ybbP"
                     /locus_tag="ACHOQY_003873"
     CDS             7720..10134
                     /gene="ybbP"
                     /locus_tag="ACHOQY_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9679881.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2256 a         2637 c         2864 g         2518 t
ORIGIN      
        1 taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg atgttttccg
       61 gttatgccta tgcgcgtctg ggggcgagct atcccagcaa tggcggcatt atcgacttct
      121 ttcgtcgcgg attaggcaac ggcgtctttt cgctggcgct ctcgttactg tacctgttga
      181 cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt
      241 tgcatgaagg cagccaggag gagcacctta ttttgctcta cgcgttgggg atcattgcgg
      301 tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg
      361 tcggcattaa aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac
      421 cggcgcatat ttccgtctct gcgcccccca gctccggtgc gttcttctcc tgtatcggga
      481 taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag
      541 atccgcaggt cattatgccg cgggcgtttc tggtggcgat tggcgttacc acgttgcttt
      601 atatctcgct ggcactggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg
      661 ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg acatgtgggt tatgtgatcg
      721 tcgtcatcgg cgctttactg gcgacggctt cagccattaa cgcgaacctg ttcgccgtgt
      781 ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg aataaatccc
      841 tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt gttgattatg ctgatgacgg
      901 cggcactaaa tttaggctca ctcgccagcg ttgccagcgc cacctttttg atttgctatc
      961 tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc
     1021 ttatcgttgg tacgttggtg atgttgttgg tgatcgttgg ctttatctac agtctgtggt
     1081 cccagggtag ccgtgcgttg atatggatta ttggctcact cttactcagc cttattgtgg
     1141 caatggtcat gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt acttcctgta
     1201 ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa
     1261 accctttaac aaagcacagg aggcgttgcg cgaacgatga acatcagcga tgtagcaaaa
     1321 attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg
     1381 ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa cgaactgacc
     1441 ttactgcgcc aggcacggca ggtgggcttt aacctggaag agagcggcga gctggtgaat
     1501 ctgtttaacg acccgcagcg gcacagcgcc gacgtcaaac ggcgcacgct ggagaaggtg
     1561 gcggagatcg aacgacacat tgaggagctg caatccatgc gcgaccagct gctggcactg
     1621 gcgaatgcct gccctggcga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc
     1681 tgctgtcatc atcgggcagg gtgattaaga cgagacggct cggatatgta gggttatccc
     1741 ttctatcgca atgacttcaa catgcgtacc tgcaccgaga tcctcgctgg cgctgacagg
     1801 ccatgaactg tcaccgacgc gcatatgacc gcgcccgttg accagcggag attccagcac
     1861 aaaacgtcgg ccaatcagct gctgcccgcg ctggtttaaa tgactatcgc tgtgcttttg
     1921 ttcgcgcacc cgccgcgaca accatttcca ccacagccag gcggcgagca gcgtcaggat
     1981 ggcaaacatt accccttgcc actcccaacc cagcggcacc agccagacca ccaggctggt
     2041 aatcaccgct gccacgccgc tccacaacaa ataaccattt ccgcccagca tctcggctgc
     2101 cagcagcaaa ccgccgagac tgagccagaa aatatgtgga tgaacgacca ttaactccat
     2161 catggctgag tccgtttgtt ggcgctgtct ttcaccagct cggcaatccc ggcaatcgac
     2221 cccatcaggc tgctggcctc taatggcatc atcactactt tgctgttact ggaggaaccg
     2281 atctgctgta acgcttcggt gtatttctgc gctacgaagt agttcaccgc ctgaatatca
     2341 ccggaggcga tagcttcaga caccattttg gtggcgcggg cttctgcttc ggcggaacgt
     2401 tcacgcgctt cagcctgtaa aaacgccgac tgacgttcgc cttccgcttt caggatttgc
     2461 gactgttttt caccttcggc tttgaggatt tccgcctgac ggatcccttc cgcttcaaga
     2521 atgtaagcgc gtttggtacg ttccgctttc atctgcgcgt tcattgaaga gataagctct
     2581 gccggtgggc gcacgtcgcg aatttcaata cgggtgactt taatccccca cgggttggtg
     2641 gcttcatcga caatacgcag caggcgtgag ttgatgctgt cgcgctgaga gagcatttcg
     2701 tcaagttcca ttgaacccaa cacggtacgg atgttagtca tggtcaggtt gatgatcgcc
     2761 agctccagat tgctgacttc ataagccgcg cgcggcgcgt caatcacctg aataaaacag
     2821 actgcgtcga tggtaacgtt ggcgttatct ttcgagataa cttcctggga agggatatcg
     2881 agcacttgct ccatcatatt gatcttgcga ccaatgcgat ccataaacgg caccaccaga
     2941 ctgagccccg gctgtaacgt tttggtatag cgaccaaaac gttctaccgt ccattgatag
     3001 ccctgcggca cgattttgac gcccgcgccg acaatgacca gcgcgacaaa aatgagaatc
     3061 gggataaaga taagcatcgg aaaaacctcc tgttgtaccg tccataatca gcaaaattgc
     3121 tgcttgatta aacaaattat acctgattac tgaaagagag ttccccctta ttcctgcgaa
     3181 ggataaactg tttttagtaa aaatcagaaa aagggaacag tgatgcagga aaatagtcct
     3241 ttacttcagc tacaaaacgt aggatatctg gcgggtgatg cgaagattct taataacatc
     3301 aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg gtccttccgg ttgtggcaaa
     3361 agtacgctgc taaaaatagt tgcttcattg atcagcccaa ccaacggaac gttactgttt
     3421 gaaggtgagg atgtcagcac actaaagcca gaaatctacc gccagcaagt ctcttactgc
     3481 gcccagacgc cgacgctgtt tggcgatacg gtatacgata atctgatctt tccctggcag
     3541 atccgtaacc agcagcctga cccggccatt tttctcgatt ttctcgaacg cttcgctttg
     3601 ccggacagca ttttgacgaa aaatatcgcc gagctatctg gtggtgaaaa acaacgcatc
     3661 tcactgattc gtaacctgca atttatgccg aaggttttat tgctggatga aatcaccagt
     3721 gcgctggatg aaagtaataa acataacgtt aatgagatga tccatcgtta tgtgcgcgag
     3781 caaaatattg ccgtgctgtg ggtgacacac gataaagacg aaattaatca tgcggataaa
     3841 gtgattacac tgcaaccgca tgccggagaa atgcaggaag cacgctatga actcgcataa
     3901 tattactaac gaatcattgg cactggcatt aatgctggtg gtggtggcaa tcttaattag
     3961 ccataaagaa aaactggcgc tggagaaaga tattttctgg agcgtcgggc gagcgataat
     4021 tcagctgatt attgtcggct atgtgctgaa gtatattttc agcgtggatg atgccagcct
     4081 gacattattg atggtgttat ttatctgctt taatgcggcg tggaacgcgc aaaaacgcag
     4141 taaatatatt gctaaagctt ttatctcatc gtttattgcc attacggtcg gggcgggaat
     4201 taccctggcg gtgctgattc tctctgggtc gattgaattt atcccgatgc aggtgatccc
     4261 tatcgccggg atgattgccg gtaacgccat ggtagcggtg gggttgtgtt acaacaattt
     4321 agggcaacgg gtcattagcg aacagcaaca gattcaggag aaactgagtc ttggtgcgac
     4381 gccgaagcag gcttcagcga tattgattcg cgacagtatt cgcgcggctt taattccgac
     4441 ggtcgattca gcaaaaacgg ttggcttagt gagtttacct ggaatgatgt ccgggctgat
     4501 atttgccggg attgatccgg tgaaggcgat taaatatcag attatggtga cctttatgct
     4561 gctctcaacc gccagcttgt cgaccattat tgcctgctat ttaacctatc gtaagtttta
     4621 taattcgcgc caccagttgg tggtgacgca attgaagaag aaatgatgcg atgccggatg
     4681 cgcagcatcg catccggcgt tgtggttcat gtgccgatca acggaatatc aatacaacaa
     4741 tgcatacagc tggcggcgat acttcgacgc cagtgcatca cccgtaccca gcgcagcgag
     4801 gatctcctga aacgttttac gcgtctgacc gtctgcggcg gtgagatctt tacgcagatg
     4861 cccgaacagc aactccagcg cctcttcatt gcgcccaacc tggtgcagtt gcagagccag
     4921 ttgcgtcgcc agtgcggcat cttctggatt ctccgccacc tgctgttgca actgttgaat
     4981 ttccggcgta tcagccgcct gcttcagcag ttcgatttgc gccaccagcc cctggtagcg
     5041 ggtgtcctga tcctgcaacg gaatggtttt cagcaccgtt tccgcatctt cagaacggtt
     5101 cagcgcaatc agcgtttctg ccagcagcag gccgatctcc ccgttctgat tcgacaactg
     5161 ccaggcgtct ttcagcaatg gcagggcatc ggtgtaattg ccttcctgca tcagttgcat
     5221 cgcctgctgc gctttcagct cttcttcgcg cggcagcact ttatccagca gggcgcggat
     5281 cgcctcttcc ggttgcggcc cctggaagcc atctaccggt tgcccgttct ggaacagata
     5341 cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc atctgctccg cgtcgcagtc
     5401 cagcttcgcc agaataaatt gcccgttgta ctgcgccgcg aggctttcca ggatcggggt
     5461 taactgcaaa cagtgctggc tacgttcaga ccaaaaatag aacagcaccg gagtggtcat
     5521 cgactgttca agaacctgtt gcaggttaga ttcgttaatg ttgacaatat tttctacgga
     5581 catggagtcg ctctctgttg tcgatatttt ctttgacatg ggggcttaag cgcgcgcttc
     5641 aactcacccc tgcaatattt tgtccatcac gcgccccggc agcaggcgct tgagcaccat
     5701 tactgcccag gtcaccagcg ttaccggata gcgcatcttc ggcttctcgc taataaaagc
     5761 atggcgtact ttgtccacca ccgcttccgg tcccaacgta aagcgggcgg cgatgccggg
     5821 attttcgact ggtttatcac tttgcgtctg gttgacgttg tcggtgaagc gagtacgaat
     5881 gggaccgggt tcgatcaggc tgactttaat tccgctgtgg cgcagctcca tgcgcagtgc
     5941 gtctgaccac gcctccagcg catatttact ggccgcgtaa gcgccacgac ccggcgtgga
     6001 gattaatccc atcaccgatg atgtcatcac aatacgccct tcaccgtgcg gtaacatcgc
     6061 gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag ttggcggaaa actgctgttc
     6121 catctgcgca cggctgatgg tggaaagggg gccatacatg ccgaatccgg cattgttaaa
     6181 gatcccatac agacaattat cggtcagggc gatcacctcg tctgctgcgc gatcaacact
     6241 ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc atgttgttca tacgctcaac
     6301 atcatccggt tttcggcaac ctgccagcac atgaaaaccc tggcgtttta attcgagcgc
     6361 gctttccagg ccaattccac tggaacatcc ggtaattaag accgattttt gcataacttt
     6421 acctgtcagg atctccgttg ctttatgagt catgatttac taaaggctgc aactgcttcg
     6481 ccatccagtc ggcaataaac ggctgggcgt cgcggttggg atgaataccg tcatcctgca
     6541 tccattgtgg cttgaggtag acctcttcca taaaaaaggg cagcagcgga acatcaaact
     6601 ctttggcgag tttggggtaa atggcgctaa aggcttcatt ataacggcga ccatagtttg
     6661 caggcagacg tatttgcatt aacaatggtt cagcgttggc ggctttgaca tcctgcaaaa
     6721 tctggcgcag cgtttgctcg gtttgctgtg gctgaaaacc acgcaaaccg tcattgccgc
     6781 ccagttcaac cagcacccag cgcggctgat gctgtttcag cagagccgga aggcgcgcca
     6841 gtccttgttg cgaggtgtcg ccgctgatgc tggcattaac taccgacgtt ttactctgcc
     6901 acttatcatt caacaaggca ggccaggccg cgctggcaga cattcgatac ccggcgctca
     6961 ggctatcacc cagaatcaat aacgtgtccg ctgcggcggc acggaaggtt aacaggacca
     7021 ggaacaggaa gggcaaatgc cagcggaaaa cattgttgaa gttcatcatc ttaagaagtc
     7081 cgtcggtcag ggggagcatg aactctccat cctcaccgga gttgagctgg ttgtcaaacg
     7141 tggcgagacc atcgcactgg tgggcgaatc gggatcgggt aagtcaacct tgctggcgat
     7201 cctcgccggg cttgatgacg gcagcagtgg cgaagtgagt ctggtgggac aaccgctaca
     7261 taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag cacgtcggct ttgtttttca
     7321 gtcatttatg ttaattccta cccttaacgc gctggaaaat gtcgagcttc cggctctgct
     7381 gcgcggtgag agtagcgcgg aaagtcgtaa cggggcgaaa gcgttgctcg aacagttagg
     7441 gctgggtaaa cgtctggatc atcttccggc acagctttcc ggcggtgaac agcaacgagt
     7501 ggcgctggca cgagccttta atggtcgacc tgatgtgctg tttgccgacg aacccaccgg
     7561 caaccttgac cgccagacgg gcgataaaat tgccgacctg ctgttttccc tcaaccgtga
     7621 acatggcacc acgttgatta tggtgactca cgacctgcaa ctggcggcac gctgcgaccg
     7681 ctgcttacgg ctggtgaacg ggcagttgca ggaggaagca tgattgcacg ttggttctgg
     7741 cgcgaatggc gctcgccgtc gctattaatt gtctggctgg cgctaagcct ggcggtggcc
     7801 tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga agggcttaag ccagcaaagc
     7861 cgtgagttta tggcgggcga tcgggcgttg cgcagttcgc gcgaagtgcc gcaagcatgg
     7921 ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc agctgacttt cgccaccatg
     7981 acatttgcag gcgacacacc gcagctggcg aacgtcaaag cggtggatga tatctacccg
     8041 atgtatggcg atctgcaaac taatccccct ggcctgaaac cgcaggcggg cagcgtattg
     8101 ctggccccac gcctgatggc actgcttaac ctgaaaacgg gcgacaccat tgacgtgggc
     8161 gatgccacct tgcggattgc cggagaagtg attcaggaac cggattccgg ttttaacccc
     8221 ttccagatgg ctccgcgtct gatgatgaat ctggcggatg tcgataaaac cggagccgtg
     8281 cagccgggga gtcgggtcac ctggcgttat aaattcggcg gcaacgagaa ccagctcgac
     8341 ggctatgaga aatggttgtt acctcagctt aaacccgaac aacgctggta cggtctggaa
     8401 caggacgaag gcgcgctggg gcgatcgatg gaacgctcgc aacagttcct gctgctttcg
     8461 gcgcttctga ccttgctgct ggcagtggca gcggtggcgg tagcgatgaa tcattactgt
     8521 cgcagtcgct acgatctggt ggcgatcctc aaaacgctgg gggcagggcg agcgcaactg
     8581 cgtaagctaa tcgtcggtca gtggttgatg gtgctggcgc tttcagccgt taccggtggg
     8641 gcgataggcc tgttgttcga aaacgtgttg atggtgctgc tcaagcccgt tctgcctgct
     8701 gcactaccgc cagccagcct ctggccgtgg ctgtgggcgc tgggtaccat gacggtcatc
     8761 tcgctgctgg tggggctacg accgtaccgc ctgttgctgg caacgcagcc tttacgcgta
     8821 ttacgtaatg atgtggtagc gaacgtctgg ccgctgaagt tttatctgcc gattgtcagt
     8881 gtggtggttg tgctgctgct cgccggatta atgggtggca gcatgctgct ttgggcggtg
     8941 ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc tgggctggat gctgctgaat
     9001 gtacttcgcc gcatgacgct gaaatcgcta cctctgcgcc tggcggttag ccgcctgtta
     9061 cgtcagccgt ggtcaacatt aagccagctt tcggcatttt cgctctcctt tatgctgctg
     9121 gcgctgttgc tggtattgcg tggcgatctg cttgaccgct ggcagcagca gctacctcca
     9181 gaaagcccga actacttttt aattaacatt gccacagaac aggtagaacc gctaaaagcg
     9241 ttcctcgcgg aacatcagat agtcccggaa tcattttatc cggtagtgcg ggcgcggctg
     9301 acggcgatta acgataagcc gacagaaggt aatgaagatg aggcgcttaa ccgcgaactc
     9361 aatcttacct ggcaaaatac gcgacccgat cataatccga ttgtcgccgg taactggccg
     9421 ccaaaagccg atgaagtgtc gatggaagag gggctggcga aacgcttaaa cgttgccctc
     9481 ggtgataccg tgacttttat gggcgatacc caggagttcc gcgctaaagt gaccagcctg
     9541 cgcaaagtgg actgggaaag tctgcggcct aatttctatt ttattttccc tgaaggggca
     9601 ttagacgggc aaccgcagag ctggcttacc agtttccgct gggagaatgg caacggcatg
     9661 ttgacacaac tcaaccgcca gttcccgacc attagcctgt tagatattgg cgcgatttta
     9721 aaacaggtcg gtcaggtgct ggagcaggta agtcgggcgc tggaggtgat ggtggtgctg
     9781 gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc aggtgggaat gcgccagcgt
     9841 catcaggagc tggtggtatg gcgcacactc ggtgcgggga aaaaactgct gcgcacgaca
     9901 ttgtggtgtg agttcgccat gcttgggttt gtttccggcc tggtggccgc aattggtgcg
     9961 gaaacggcac tggcagtgtt gcaggcgaaa gtgtttgatt tcccgtggga gccagactgg
    10021 cgattgtgga ttgttctgcc gtgcagcggt gcgctgctgc tgtcgctttt cggcggctgg
    10081 ctgggtgcgc gactggttaa gggtaaggcg ctgttcaggc agtttgcggg gtgatgagag
    10141 taaataaggg gcgttctggt tgaatggaac gccttgttta aattaattct acaatatgtt
    10201 tcattgatga tgttattgaa ttggtgattt cctatctttc tattgcgata ttaatctgaa
    10261 tcggtggagt ttaat
//
LOCUS       DAZKGS010000152        10203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKGS010000152 DAZKGS010000000
VERSION     DAZKGS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..178
                     /locus_tag="ACHOQY_003874"
     tRNA            92..178
                     /locus_tag="ACHOQY_003874"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Leu,seq:cag)
     gene            complement(217..453)
                     /gene="yjjZ"
                     /locus_tag="ACHOQY_003875"
     CDS             complement(217..453)
                     /gene="yjjZ"
                     /locus_tag="ACHOQY_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9679882.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            594..1382
                     /gene="fhuF"
                     /locus_tag="ACHOQY_003876"
     CDS             594..1382
                     /gene="fhuF"
                     /locus_tag="ACHOQY_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9679883.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1420..2097)
                     /gene="bglJ"
                     /locus_tag="ACHOQY_003877"
     CDS             complement(1420..2097)
                     /gene="bglJ"
                     /locus_tag="ACHOQY_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9679884.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(2055..2780)
                     /gene="yjjQ"
                     /locus_tag="ACHOQY_003878"
     CDS             complement(2055..2780)
                     /gene="yjjQ"
                     /locus_tag="ACHOQY_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9679885.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3399..4169
                     /gene="yjjP"
                     /locus_tag="ACHOQY_003879"
     CDS             3399..4169
                     /gene="yjjP"
                     /locus_tag="ACHOQY_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9679886.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            4160..4633
                     /gene="yjjB"
                     /locus_tag="ACHOQY_003880"
     CDS             4160..4633
                     /gene="yjjB"
                     /locus_tag="ACHOQY_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9679887.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4740..5279
                     /gene="dnaT"
                     /locus_tag="ACHOQY_003881"
     CDS             4740..5279
                     /gene="dnaT"
                     /locus_tag="ACHOQY_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9679888.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5282..6019
                     /gene="dnaC"
                     /locus_tag="ACHOQY_003882"
     CDS             5282..6019
                     /gene="dnaC"
                     /locus_tag="ACHOQY_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9679889.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            6068..6562
                     /gene="yjjA"
                     /locus_tag="ACHOQY_003883"
     CDS             6068..6562
                     /gene="yjjA"
                     /locus_tag="ACHOQY_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9679890.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6816..9107
                     /gene="opgB"
                     /locus_tag="ACHOQY_003884"
     CDS             6816..9107
                     /gene="opgB"
                     /locus_tag="ACHOQY_003884"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9679891.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9246..>10203)
                     /gene="lgoD"
                     /locus_tag="ACHOQY_003885"
     CDS             complement(9246..>10203)
                     /gene="lgoD"
                     /locus_tag="ACHOQY_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9679892.1"
                     /translation="PVPGDNEALIKIKTVGICGTDIHAWAGNQPFFSYPRVLGHEICG
                     EIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGRTNCCEKISVIGVHQDGGFSEYL
                     SVPVANILPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAIA
                     KADGAQVVVADTSPARREHVATRLELPVLDPSAEDFDAQLRAQFGGSLAQKVIDATGN
                     QHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDFAKVGRL
                     MAEGKITADMMLTHRYPFATLAETYERDVINNRELIKGVITF"
BASE COUNT         2588 a         2632 c         2451 g         2532 t
ORIGIN      
        1 agcttcaggt gttagtgttc ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa
       61 cgaggcgata tcaaaaaagt aagatgactg tgcgaaggtg gcggaattgg tagacgcgct
      121 agcttcaggt gttagtgtcc ttacggacgt gggggttcaa gtcccccccc tcgcaccaat
      181 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattag
      241 catcagcatg atgccagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      301 ctgtttgtga tacagcatca ccggcgtcaa tatcaatccc atcagaatga caatccgcca
      361 ttgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      421 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      481 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      541 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      601 atcgttccgc accgctctat gaagatgtca tctggcgaac gcatctccag ccgcaggatg
      661 ctgggcttgc acaagccgta cgggcgacga tcgccgaaca tcgtgaacat ttgctggagt
      721 ttatccgtct ggatgaaccc gccccgcttc gcgccatgac gctggcgcaa tggtcatcac
      781 cgaatgcgct aagttctcta ctggcggtct attccgatca tatctatcgc aaccaaccaa
      841 cgatgatccg cgagaacaag ccgctgatct cactatgggc gcaatggtat atcggcctga
      901 tggtgccacc attaatgctg gcgctgctga cgcaggaaaa agcgttagat gtgtcgccgg
      961 aacatttcca cgttgagttt cacgaaaccg gccgtgccgc ctgtttctgg gtcgatgtgt
     1021 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
     1081 aggcgctggt tccggttgtg caggcactag aagcgacggg agagatcaac ggcaaactta
     1141 tctggagtaa taccggttat ttgatcaact ggtatctgac tgagatgaaa cagctgctcg
     1201 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg cttactaccg
     1261 gtgaagataa tccactctgg cgtaccgtgg tactgcgcga cggcctgctg gtgcgccgca
     1321 cttgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1381 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1441 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1501 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1561 acgtttaagc tgctcggcaa tttgtgtcat tgagtaacca cgcgacataa agcgcaatat
     1621 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1681 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1741 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1801 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaagct taatcgccag
     1861 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1921 gaccgccgca aacgagatcc gcgtcatggc atcctgaaat gactcctgac tggtataggt
     1981 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactact
     2041 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     2101 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     2161 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
     2221 gcgctgatag ttttattact tttcttaagc aatgaagcta tctggttgat gccccatcct
     2281 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
     2341 aaatcatgag tttcagtcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc
     2401 gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
     2461 gtcgccgtag ggcacatgag cagttctact gcctgggaat accaggagcg tgacaccatg
     2521 aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa
     2581 cgtggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg gcgtaattgt
     2641 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
     2701 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
     2761 ttgcagcatc ctggcaacat attccctcca catccactgt ctaaaaatta tgatggttta
     2821 ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat
     2881 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt
     2941 cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta
     3001 cattaagttg ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt
     3061 aaatgatatc ccttcctgca gaatgcaaaa tcacacaacc aatatttatt caatgaaatc
     3121 tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt
     3181 aaaggccaaa caataaattc attatgatga taatattcat gtgatagttt gtttgcggcg
     3241 agagataatt cgctttttat caccgagctt ctcccgataa ccaggattaa tcagcgcaat
     3301 tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
     3361 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3421 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3481 cgttggttga tgagctttcc tcacgactgg gtcgggcgct gggaatggac agcgtcgaaa
     3541 gttctatctc ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat
     3601 cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3661 ttgtgattct tgcggagcat catctgctgg attacaaagg cgtagagaaa cgatttagcc
     3721 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg
     3781 cctgtttctg taaactcaat aacggtggct gggatggtgc cgtcatcacc ttctttgcca
     3841 gtacgaccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca
     3901 acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg caactcccca
     3961 ctttcagcga tacacccacc attgcgatgg ccgccagcgt tctgctgctg gtgccgggct
     4021 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     4081 gctgggcgat cgccagtctg ctgacgctgg ctacctgcgt cggcgtggtg atggcactga
     4141 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4201 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc
     4261 gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac
     4321 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4381 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat
     4441 ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4501 gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4561 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4621 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4681 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4741 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4801 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4861 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4921 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4981 ccgttgctgt ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     5041 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     5101 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     5161 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5221 gagatgtgaa tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac
     5281 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5341 acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag gggcgatccg
     5401 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5461 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5521 gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc
     5581 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5641 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5701 ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct
     5761 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5821 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5881 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5941 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     6001 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     6061 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     6121 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6181 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6241 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6301 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6361 ccaggtgctg gaaaagctgg gcctgaacag tgaagaacaa aaagaagaca ccaactatct
     6421 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6481 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6541 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6601 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     6661 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6721 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     6781 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6841 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6901 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6961 actattttac tggcgctggt attaacgacg cggttctcta taccttaacc aacagcctga
     7021 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     7081 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg
     7141 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7201 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7261 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7321 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7381 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccgggaacag
     7441 attacacgat tgcgggcatg gtggcttctc agtgcggcat tccgcttttt gccccatttg
     7501 aaggtaacgc ctccgcttct gtttccagct tcttcccgca gaacatctgt ctgggcgata
     7561 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7621 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7681 aaagcgtggt agccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7741 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7801 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7861 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7921 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtca
     7981 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     8041 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     8101 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     8161 actatctcgg acttggtcgt agcagtttat ccgggcagtc aatgtcggaa atcttcctca
     8221 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8281 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8341 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8401 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8461 tctgggttga ccgttgctac aagatggcac aactctgggc tccggaactg gcactttcca
     8521 ccgactggtg tgtctcgcag gggcagcttg gcggtcagca aattgttcag catgttgaca
     8581 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttaca
     8641 aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt
     8701 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8761 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8821 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8881 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8941 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     9001 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     9061 tcgggatcgg catggtggaa attaaagttg tggaacgtga agggtaattc ctgactgatg
     9121 tgtaggtcgc ataaggtgcg acctacacac cttctccggc ctacaaaaca tcattgagaa
     9181 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9241 ccttatcaga aagtaattac acctttaatt aactcacgat tgttaatcac atcgcgctcg
     9301 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9361 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggctg
     9421 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9481 aacaggccga caaataccac cgtgccgccg tgacgaatca gattcacggt gttattcatc
     9541 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9601 cgcaactgcg cgtcaaaatc ttcggctgac gggtccagca cgggcaactc cagacgcgtt
     9661 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9721 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9781 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9841 gcggccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9901 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9961 gtacgcccgc ttttacacgc cgggcattgc tgacaagaaa tatacggaat taccgcaact
    10021 tgctgtccga tattaaaatt gtgaaagttt tttcctgatt caataatctc cccacatatt
    10081 tcatggccta aaacgcgtgg atagctaaaa aatggttgat tgcccgccca ggcatgaata
    10141 tctgtaccgc aaatcccgac ggttttaatt ttaattaatg cttcattatc gcctggcacc
    10201 ggt
//
LOCUS       DAZKGS010000153        10179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKGS010000153 DAZKGS010000000
VERSION     DAZKGS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..233)
                     /locus_tag="ACHOQY_003886"
     CDS             complement(<1..233)
                     /locus_tag="ACHOQY_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679893.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLC"
     gene            334..1504
                     /locus_tag="ACHOQY_003887"
                     /pseudo
     CDS             334..1504
                     /locus_tag="ACHOQY_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1587..1844
                     /locus_tag="ACHOQY_003888"
     CDS             1587..1844
                     /locus_tag="ACHOQY_003888"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679894.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(2402..3169)
                     /gene="fecE"
                     /locus_tag="ACHOQY_003889"
     CDS             complement(2402..3169)
                     /gene="fecE"
                     /locus_tag="ACHOQY_003889"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFP9679895.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(3170..4126)
                     /gene="fecD"
                     /locus_tag="ACHOQY_003890"
     CDS             complement(3170..4126)
                     /gene="fecD"
                     /locus_tag="ACHOQY_003890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFP9679896.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(4123..5121)
                     /gene="fecC"
                     /locus_tag="ACHOQY_003891"
     CDS             complement(4123..5121)
                     /gene="fecC"
                     /locus_tag="ACHOQY_003891"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFP9679897.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLAMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRIPLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(5118..6020)
                     /gene="fecB"
                     /locus_tag="ACHOQY_003892"
     CDS             complement(5118..6020)
                     /gene="fecB"
                     /locus_tag="ACHOQY_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFP9679898.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(6065..8389)
                     /gene="fecA"
                     /locus_tag="ACHOQY_003893"
     CDS             complement(6065..8389)
                     /gene="fecA"
                     /locus_tag="ACHOQY_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFP9679899.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(8476..9429)
                     /gene="fecR"
                     /locus_tag="ACHOQY_003894"
     CDS             complement(8476..9429)
                     /gene="fecR"
                     /locus_tag="ACHOQY_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFP9679900.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(9426..9947)
                     /gene="fecI"
                     /locus_tag="ACHOQY_003895"
     CDS             complement(9426..9947)
                     /gene="fecI"
                     /locus_tag="ACHOQY_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFP9679901.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            10084..10152
                     /gene="ykiE"
                     /locus_tag="ACHOQY_003896"
     CDS             10084..10152
                     /gene="ykiE"
                     /locus_tag="ACHOQY_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFP9679902.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
BASE COUNT         2314 a         2774 c         2873 g         2218 t
ORIGIN      
        1 ttacagagat tgctggtaag tgattgccca ttttttaaca cgcttacctt actaaacgga
       61 atctcacttt tttctctatg ctccgcattt actagtggag gatcaaacac aacctcaaca
      121 ttataggcag gtgcgttccc tgtattagca atgttaatat caaagtaaaa agcggcccaa
      181 gggtttggct caagtgttac aactatatgg ggttgagctg atgcgtcaac cattcttttt
      241 gtctcgtctg ctctgaagtg gtgaagtggt ttactgaatt tggccacctg aacagaggtg
      301 atatgctcac ctcagaacaa cacaggtgct ccaatgaaaa aaagaaattt tagcgcagag
      361 tttaaacgcg aatccgctca actggttgtt gaccagacat acacggtggc agatgccgcc
      421 aaagctatgg atgttggcct ttccacaatg acaagatggg tcaaacaact gcgtgatgag
      481 cgtcagggca aaacaccaaa agcctctccg ataacaccag aacaaatcga aatacgtaag
      541 ctgaggaaaa agctacaacg cattgaaatg gagaatgaaa tattaaaaaa ggctacaacc
      601 gcgctcttga tgtcagactc cctgaacagt tctcgataat caggaaactc agagcgcatt
      661 atcctgtggt cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga
      721 aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt acgcagccag gtacttgaac
      781 tgcatggcat cagccacggc tctgccggag caagaagcat cgccacaatg gcaacccgga
      841 gaggctacca gatggggcac tggcttgctg gcagactcat gaaagagctg gggctggtca
      901 gttgccagca gccgactcac cggtataagc gtggcggtca tgagcacgtt gctatcccga
      961 atcatcttga gcgacagttc gccgtaacgg aaccaaatca ggtgtggtgc ggtgatgtga
     1021 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
     1081 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggcttacc atgaaagcac
     1141 tggaaatggc atgggaaacc cgtggtaagc ccgtcggggt gatgttccag gcgatcaagg
     1201 cagtcattat acgagcaggc agttccggca gttactgtgg cgataccgga tcaggcagag
     1261 tatgagtcgg cgtggaaact gctgggataa cagcccaatg gagcgcttct tcaggagtct
     1321 gaagaacgaa tgggtgccag cgacgggcta tgtaagcttc agcgatgcag ctcacgcaat
     1381 aacggactat atcgttggat attacagcgc actaagaccg cacgaatata atggtgggtt
     1441 accaccaaac gaatcagaaa accgatactg gaaaaactct aacgcggagg ccagttttag
     1501 ttgaccacaa cagactacct gaagggagcc gcggtcgcct agcagttgca gtagcaggag
     1561 atcatccagc cgcagtacag gtcacgatga ctctggttaa tgataccggc tttgaccccg
     1621 tattttccgg ctctatcgct gaatcatggc gtcagcagcc gtgcacacca tcctattgtt
     1681 gtgactggga ggctgccacc atgcttcgcg ctttccctct ggcgaaaaag ggagaaggac
     1741 gggcccgtct gccttcactt tatgccagct tcggtaagct gggtgagaca ccgactcata
     1801 aagatatcat tgataacaat cgatccatca actggcctgt ataacgtggc tgccggtgat
     1861 taagaaagct gcacctacct aagtagtagc aaacgcacac tttttagaaa aatcgatggt
     1921 cagaaactgg attagcaatt ccgttccagg gttgcttttg atttacgttg gcgtctgatc
     1981 attgatttat cctcaaaagc ccaacctcat tggtaatgaa ccagctccgt gaatgtccgc
     2041 tctggcacag agcgaaattt tttgatctcc ccccctgaaa tctaaactta gtcatgtcac
     2101 gtttttgggt ttctaaaatt ttaacttcgc gtttttcgtt gccgtaaggg ttatacagaa
     2161 atgtccgtta agcagagttc aaaattgatt gccgtgatca cgactggttt gaaagccgcg
     2221 cccaagcctg tacagctctg gtttgcgttg attatgaacc tgtcagccta aagcaagcgg
     2281 atggacgatg agtattggta atctttcaga gtccggaaaa gttcagcccc agtctgaaca
     2341 ggcttgctgg cgccagtcca gtttcattca gtcgtggttt ggttcttacg gcctgtgcaa
     2401 tctacctcat taggcacatc ggcctgccag ataccggctc ggggtgtatt tccgcttcca
     2461 cgctgaatac tgttctcagc aatcctgggg tcatcacctc ttctggtgtg ccttgcgcca
     2521 taacatgtcc gtttgccatt accaccagtt gatcgcagta ccggctagcc tgattaaggt
     2581 cgtgcagcac agcgaccacc gttttcccct gagtccggag ttcgcccatc aaccgcatca
     2641 ggtccacctg gtgattgata tcaagatagg tggttggctc atcaagtaat acaacgggcg
     2701 tattctgggc caggaccatc gccagaaatg cgcgctggcg ctgaccgccg gaaagctcgg
     2761 ttaaccgacg aacggcaaga tgattgatcc gggtctggtt catggcgaca ttaactcgtg
     2821 cattgtcttc agcggagaga cgcccccaga gtgacagcca gggattacga ccatacgaaa
     2881 ccagctcctg gactgtgatc ccctctggcg ttaaatggtg ctgaggcagc agcgaaagcc
     2941 tgcgggccaa ctggcgcgat gagagcatat ttatgggatt atcgccgaga aatacggtgc
     3001 cagactgcgg cattaaaagc cgcgaaaaac agtttaacag cgtcgatttc ccgcaaccgt
     3061 taggaccgat cagggcggtg atcttccccg ttggcagtga gagtgaaacg tcgttaagta
     3121 ccttgtctgt cccgtaactg accgtcagat tttcagttcg taaagtcatt tatcgcattc
     3181 tcacaagcaa ccagacaaac cacggcgcac cgataatggc ggtcagcacg ccaaccggga
     3241 gctccagtgg gggatgaata attctcgcca gcagatcggc aaccaccaac agcaacgcac
     3301 ctgtcagggc tgaaacaggc agcagtctgc ggtgacgtcc accggtgatg ctacgcatca
     3361 tatgcggcac cacgagacca ataaagctaa tcgggccgca ggcggccacg ccggtagatg
     3421 tcatggcgac agctagtaac aaagcccaga atcgggtatg gggcaccgac acaccgagcg
     3481 tggtggcgcg cgcatcgccg agtgcaagga ggtcgagatc gcggcaaaaa ctcaggctca
     3541 gcggcagaaa taaaatcatc agcgggatgg caatcttcac aaagctccag tcacggcccc
     3601 ataagctgcc ggtcagccac agcagggcgt tgttcacatc ctgtgggcgc gagagcatca
     3661 gataatccgt caggctggcc cagcatgcag aaagcgccac gccggtgagc gccagcttca
     3721 tcggctggtg ggtctttgcc agcatcttca gtaatatcaa ccccgccatg ccgcccgcaa
     3781 aggccagcag cggcagcacc atcacgggca gtgacggcat aagaagtaga gcccccacag
     3841 aggccaggct ggcggcatgg ttaacgccga gaatatccgg tgatgccaga gggttgcgca
     3901 caatcccctg tatcagcacg cccgccacgg cgagggctgc accgacaaac agtgccagca
     3961 gcaagcgcgg cagtcggtac tccatcaata cataataatg ctcgcgtccg gcctgccagt
     4021 cggtcagcag cgcgcgccac ggcacgggga tcactcccat atggagtgat aacagcgcac
     4081 agcccgccag ggcaagggtg atgaaaataa ccagcgcaat tttcatcctc gcctcctcac
     4141 aagccagaca aagcaagggc tgccaatcag cgccagcact gcgcctgcgg gcagatctcc
     4201 ggggaaggcc agcgcgcgtg cgagtacatc tgccagcagc atcagcgtgg cccccagcag
     4261 catgctcact ggcagtacgt tgcgctgatc gaagcctgcc cagaagcgcg ccagatgtgg
     4321 caccagcaga ccgataaacg ccaccggacc tgccacgctg acgcacgcac caaccagaag
     4381 cagcactaac atattgatga ccaaacgtag cctcgtcagg ttcactccca gcgtatgggc
     4441 ggtgctgtcg ctgaggttga gcaggttcag ttgattcgcc agcagcaaca cgacagggac
     4501 tgcagtgacc accaccggca agagctgcca gacatcctgc cagcgggcgt gggacactcc
     4561 gcctgccagc cagtaaaaga tgccgtaagc atgatcttcg gccagcagca gggggatgcg
     4621 ggtaaggccc atacaaaagg ccgacagcgc gatactcgcg aggatcagtt tgtttctgtc
     4681 atgggtatga cgaaatccgc ctcctgcggt catggccagc agccagctca cgccgccccc
     4741 gcatgccgcg atgaacgaca gagaatagcc tgcaatcggc gtcggactca gcgcgctggt
     4801 aagcgccata gccagcgccg cgccgctgtt aatgccgagc agtgaaggag aggccattgg
     4861 gttgtgggtc agggtttgca gcagcgtgcc cgcgagcgcc aggcttgcgc cgatcagaac
     4921 ggcgaccagg cttcgtggca aacgaaggtt ttgcaccagc gcttctggta gcgttggcgt
     4981 gtgtccaggc agcagggcgc gggttgcatc tgctccggaa acaggaatgg ccgagtagca
     5041 aaacagactc agccagaaaa taataataag tgctgcaacg ggaagccccc acagcagcac
     5101 cgggtgtttt atcgcggtca tttcacaacg gtaagcggct gatggtggaa gatttttacc
     5161 gtgtcagcgg caatacgctc tgcagcaaaa ataccgcgca tccgcgccca ggtgttactg
     5221 tcgaccgaag caacctgctg cttctgcgcg gcggttaaca tctgccagag cggatcttgt
     5281 tgccagcgtt taacaatgct ctcttcgcga tagtgggcaa ccagcagcca ggcaggattg
     5341 accgccagca gttgctccag gccgatggac ggcatggacg cacccgccat cgcagcggga
     5401 acgttcagcc ccagagaggc cagcacgctg ccggtccagg tctcctgagt atgcaggttg
     5461 aattgctgtt cgcgtgatgt gccaaaggcc acgcgtgtcc ctttgggaag ctggctggcc
     5521 cactgcgcca tcctctcttt atgttgttcc aggcgtgcct gcatctctcg ctttttaccc
     5581 accatttcgc cgatgatagc cgcagattgc aaattttcag cgtaggtttc gttgcgggac
     5641 ttaagcagca gtaccggcgc gatttgctgc aaggcgatgt aaacccccgc atggcgactg
     5701 ctgtcggcaa tgatcaggtc tggtttcaga gcggcaatgg cttccaggct cggctgcgcg
     5761 cgcgttccga cggactgcca cggtttcagg tgcgcacgca cttcgggcag gatgcgtttt
     5821 gcatcgttat cgtcggcaat accgatcggg ctgacgtcca cggcggccag cgcatcggcg
     5881 aacgagagtt ccagcaccac aatccgttgt ggcgtttttt cgagtgtaaa cgtgccgtgt
     5941 tcgtcctgaa ccgtggcggc aaaggcgtgg ctgatcacca gcagcaggcc tgcaaaaaca
     6001 aaacggataa atgccaacat aatcacattc cagctaaaag cccggcaagc cgggcgttaa
     6061 cacatcagaa cttcaacgac ccctgcatat acagcgtgcg cggctggcct gcatagatgc
     6121 ctttgttgtt gtcgtcataa gagcggatga agtagtcctg gtcgaagatg tttttcacac
     6181 cgaatgccag gttcagatct gccatctgcg ggccaaaatc atacgccacg cgtgcgcccc
     6241 agagcatgaa gccgggaatg cggccggtac tgccgtcggc gctctctttc accgtattgg
     6301 cgttatccgc aaactggctg gactggaaat cgctgttcag attgaacgtc cagtttcctg
     6361 gcttgtagtc cacgcccagc gtgcctttat gtttcgggga gaatggtacc agattgccgt
     6421 aggtgtcgcc tttctcgcgg atttccgcgt tcacatacgc atagctggcg tagatggaaa
     6481 cgttatcaag cgttggcgtt agcgtaccca gatcgtaacg tgcctgcgtt tccagcccgg
     6541 tatggcgcgt tttgccacgt gcagtgacgg tgtcgttggt ctggttggag tcgtactgat
     6601 tgttaaagtt aatcaggaac agccccattt ccgccgtcag cgcgccgtcg tcgtagcggg
     6661 taccgagttc ccaggttcgc gctttttccg gttccacatt gccgctttgc acagccttgc
     6721 caatctggct gtactgtacg gtgccgaacg agccttcagt gtttgcataa agattccagc
     6781 tgtcagtcag gtgatagagc acgttcaacg ccggaagcgg tgcgttatag ctcacttctt
     6841 cgtgcgtgcc tgtgatggcg ttgttctggt atgactcgat atgttcgaaa cgcatacccg
     6901 gcgtgatggt ccagttgccg atgtcgattt tgtcatccag ataccaggcg tgcgcctcgg
     6961 tgccggaacg cgtatcgcgg tcgtaagggc ttgagccgga cggcaactgc ccgctgctgg
     7021 tggcggtgta gtaacgcatt tcatgcgttg attcattcaa atagcgatag cccacgccca
     7081 cttcgtgcgc ggaagggccg atcataaaga tctggctgta gcgtggctca ataccgcgca
     7141 cccagtagtt acgcggcgag agggtgatgc gtttgccttg ctccaggtag ccgctgcgca
     7201 gggtttgggt gtagaacccc tgaatgttga atttatgctg gctgtctggc tggaactggt
     7261 agcccaggct cgccagcttg cgacgacccc agaagcggtc atacgggcgg gtggattgcc
     7321 agcgatcggc gtcgtaatcc gcgcgagaca ggccaccggg catgtcggct tcaccatcgt
     7381 aatattgcag caggctgttg aaggtgtgca cctcatccgg cgcatatttg cttttcagca
     7441 tcaggtcgtc gatgcgggtg gcgctgtgct cgcgccagtc actgccgcgc gtgccggagt
     7501 agagcagcgc ggtgccaaaa ccgttgtccg ctgtgccgcc caccatcagg ttgtgcgtct
     7561 ctttcgggtt gttttgtgaa gaggttgggc tgagctgacc ttccacgccc gcctcgatac
     7621 caaagtcctg cggaatggca cgggtaacaa agttcaccac gccgcccacg ctctgcggtc
     7681 cgtaacgcac cgcaccacca ccgcgtacca cgtcaatggc atccatgttg ccgagcgaaa
     7741 cgggagccag tgaaagctgc ggctgaccgt aaggggcgaa ggggacgggg atgccgtcca
     7801 tcaggacggt cgagcggctg gcgaggcgcg ggctcaggcc ccggatgcca aagttcatcg
     7861 ccaggtcgtg gctgccggtg ccgttgtttt ccggcgcgct gacgccaggg atgcggttaa
     7921 gtacctcacg catggtggtt gcgccggttt tggcgaaatc ctcacggcgg atcacgtcac
     7981 gcgcgccagc atgttcaaat acgtcgtttt cacgcgcatc acccagccag tcgccgacca
     8041 cggtcagggc atcttctttt ggtgcgggcg cgggctccag cgtccagctg ttatttccca
     8101 gcggttttac ctgcagtccg ctgccgtcca gcagttgttg caggccgctc tcgacgtcgt
     8161 aatcgccgtg caggccgttg ctctgcttgc cgcgcgtcag gctggcgtca accgagaggg
     8221 taaatccgct gtgtgcggca tactgattga gcgctttatc gagcgatccc ggtgcgatat
     8281 taacctgtgc agcaaaagcg gaaaacgaga gaccggccag cggcagcagg ctcaggcgaa
     8341 tggcgttaac caaaggtgtt gttttacgaa aaacgcgtaa cggcgtcata ccttccccat
     8401 catcattttt gttgtgttca gctatgagtc gaacgagaag ggtaaaaagg acaatcgaaa
     8461 taagaattat tttccttaca gtggtgaaat gtttatccag taccgcgtaa tagactgaat
     8521 tttaacggga agcgtttgcg cgataacgtt caggatcgca tcggtatttt tcagcgggaa
     8581 cgtcccgctc aggcgcagcc cggcaacggc gggatcgcag cgcagcacgc cgttgcggta
     8641 acgggttagc gtggctatca cctcacccag cggtttatcg ctgaagctca ggatgtcctt
     8701 cgtccagctt gtactctcgt catccagcgg tttcactgcg ccaaactcag aggcgctgaa
     8761 ctgcaggctt tcaccagcgt tcacgatgcg tttttgcgcg ggggcactgg cgagaagcac
     8821 ttccacagcg tgctgctgca cgtcaagctg cgtgaaatta tcctgctggc ggacggtaaa
     8881 ttctgtccct aaagcagtga gctggccctg acgggtcagg acacggaagg ggcgttgcag
     8941 ggcatctttc gcggtagtaa tggcgatttc accgtaccag agccggacgg tgcgctgatg
     9001 cgcatcaaaa cgcacatccg ccgcgctttg ggtattcagc gtgagcaggg agccatcttc
     9061 cagttgctga cggctgactg tgcctttggc ggtgcggtaa tctgcccgca gaccttcgcc
     9121 ggtttccgac tgccagagct gccagcctcc gccagcgccg agcaacagca gtaatccttt
     9181 catcacgtga cggcgggtga ggcgggtatc gtgcaacgcc ctgctggcta cgtcgccagg
     9241 cacaccgcca agctggttgc gcaggttttc aacctgctgc caggcccact ggttatcctg
     9301 atcctgttca taccactgtt gccagcgcgc ttcctgttgt gggctgacgc gctcgccgct
     9361 tagcacggca taccagtggg aagctgaacg cagcgcctga cggcgggaat cggttaacaa
     9421 aggattcata acccatactc cagacggaac agcaggcagt gctcgacggc tttcgccacg
     9481 tattttttca cggagctgat ggaaacaccg agtttgtgcg caatctcgct gtatgtcaga
     9541 ccatccagtt gcgaaagcag aaacgcttca cgtgttttgc cgtttagccc gtccagcatg
     9601 ctgtcgagga gttgtagggt ctcgagttgg ctttcgcgtt cctcaggtga aggcgctccc
     9661 ccctccggca taagcgccag catctccaga tacgcttttt ccagcgcgtt tcggcgaaac
     9721 aggtccacca tcacgcgttt ggcgatagtg cagaggaagg agcgaggatc gcggatcgtc
     9781 gagagcgttt cgctgaccat tacccgcaaa aaagtgtcct gggcaatgtc atctgcatca
     9841 aaagcagact ggagtttgcg cgtcagccag cttttcaacc agccgtgatg tgtgccataa
     9901 agcgactcga acgttaagga agctgtggta gtggcgcggt cagacatgcg gagtgcatca
     9961 aaagttaatt atcacgtagt catattaata tgagaatggt tatcattaca attggaaata
    10021 aaattgtttc caatagacat ttttaacatg ttgtttttct aagtgttata aggtaggtat
    10081 aaaatgggat ggagcctctg cttctggcat gtgtcggtca gaatgactca tgatgtggtc
    10141 tgctattatt gacatcctca ctgccctaaa ggatgggga
//
LOCUS       DAZKGS010000154        10155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKGS010000154 DAZKGS010000000
VERSION     DAZKGS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            172..996
                     /locus_tag="ACHOQY_003897"
     CDS             172..996
                     /locus_tag="ACHOQY_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9679903.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
     gene            1028..2608
                     /locus_tag="ACHOQY_003898"
     CDS             1028..2608
                     /locus_tag="ACHOQY_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9679904.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            2608..3816
                     /locus_tag="ACHOQY_003899"
     CDS             2608..3816
                     /locus_tag="ACHOQY_003899"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9679905.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            3785..4381
                     /locus_tag="ACHOQY_003900"
     CDS             3785..4381
                     /locus_tag="ACHOQY_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9679906.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            4453..5400
                     /locus_tag="ACHOQY_003901"
     CDS             4453..5400
                     /locus_tag="ACHOQY_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9679907.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(5480..5556)
                     /locus_tag="ACHOQY_003902"
     tRNA            complement(5480..5556)
                     /locus_tag="ACHOQY_003902"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5520..5522),aa:Met,seq:cat)
     gene            5765..6862
                     /gene="mltA"
                     /locus_tag="ACHOQY_003903"
     CDS             5765..6862
                     /gene="mltA"
                     /locus_tag="ACHOQY_003903"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9679908.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            6939..7745
                     /gene="tcdA"
                     /locus_tag="ACHOQY_003904"
     CDS             6939..7745
                     /gene="tcdA"
                     /locus_tag="ACHOQY_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9679909.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(7796..8239)
                     /gene="csdE"
                     /locus_tag="ACHOQY_003905"
     CDS             complement(7796..8239)
                     /gene="csdE"
                     /locus_tag="ACHOQY_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9679910.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(8239..9444)
                     /gene="csdA"
                     /locus_tag="ACHOQY_003906"
     CDS             complement(8239..9444)
                     /gene="csdA"
                     /locus_tag="ACHOQY_003906"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9679911.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            9636..9863
                     /gene="ygdI"
                     /locus_tag="ACHOQY_003907"
     CDS             9636..9863
                     /gene="ygdI"
                     /locus_tag="ACHOQY_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9679912.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         2696 a         2154 c         2491 g         2814 t
ORIGIN      
        1 atatttgaat atcggcttaa aaaataagcc tgtcatctct tttattaagc acaattccgt
       61 gtaaggtata cgcattagtt aattatcttg taactgttaa atcaggcaag gcaatgtttg
      121 aagtaattat tacttctgac atgccttgcc ttttttttgg ggagccatgg aatgatcatc
      181 gaaaaagtca tgaacaataa ttgtgtacag gcatcggtga acggacagga ggttatcatt
      241 tctgggcctg gcgttggtta caataaaaaa tatggtatgt cggtccctga gcatcctgca
      301 aaccggattt tttatgtcag aaatgagcaa aaaaataaac tctacaaatt gattgagcat
      361 gtgggtattg agtatatttt tgttgccgaa aagatagtgc attatgcgga gagtaatctc
      421 gaaaaaaaac tcaatccatc gctactactg attcttgctg atcacatttc gaatgcaata
      481 tcccgagtcg tctcaggcat acaaattaat aatgttttcc ttgaggaaat caaagcgttg
      541 tacaaagcag agtatgcaat aagtcgtgat gcattaacaa tcattaatga gcaatttagc
      601 gttcaactac cggatgatga gattggtttt atagcattgc atattttaaa taattatgaa
      661 aattcagttg attatgaatc agtaaggatt attgagctgt ctcaaaaaat cacggagctt
      721 attgaagttg tttataacag aagggtggac agaagttcat ttaactactc cagatttatg
      781 atgcacctta aatatttttc tagtcgtgtg ttatgtaatg aaaaaataaa acagaaaaat
      841 attggtgata tctatgagca gtttcttgaa aaagattttc agttacaacg tgccattcat
      901 gaaattgaaa gatatctgta cgcaactttt aaatatgatt ctcttctgga agagaaacta
      961 tatctttcta ttcgcatcaa agtattaatg gactaattat acataacatt ttcatgagat
     1021 aaccagtatg aaacaaaaga aagcctggag tttttttcag agcctgggga aggcatttat
     1081 gtatcccatc gctctgttaa gtgtatgtgg tatgatgtta gggctgggaa gtggtttagc
     1141 cagcgatgat atgggaaaat tcattccagt gttggctatt ccgttaatta aaaccatact
     1201 tgattttatt gttagccttg gtttgtttgc cttcgttaat ttacctgtat tgtttgcgat
     1261 agctatacct ttgggattat taaaagagaa agatgataaa gcctatggcg ctttttctgg
     1321 cttaattggc tttatggcga tgcatcttgg aacgaacttt tatcttaaac agcacgactt
     1381 attggtagct gctgaccaaa tgtcgacaca tgggcaaacc atcatactcg ggatccagtc
     1441 ctacaatacc agcgtgttgg gtgggattgt ggcagggtta ttagtcgcca gcatgtataa
     1501 aaagatcgtt aacttacgta ttcctgaatc attagggttt tatagtggcc cacgccttgt
     1561 gcctatcatt acactgattg tgatgagtgg atttggtctg ttcattcctt ttatctggcc
     1621 gccatttttc aatcttttca tgctcattgg tcactggatt tcaacttccg gtcctgttgg
     1681 ttatttcttc tatgctgttg ccgaacgcgt gacgatacct tttggcttaa atcacttggt
     1741 gacatcagtt ttccgcttta ccccaatcgg cggatcggca gtgattggcg gtgaagagta
     1801 ttacggcacc ctgaacatgt ttatggcata cgtcaaagag aatgcggtca ttccgctgga
     1861 tttggcgggg aaaatggagc agggcaaact gatgattcag tatggtctgg ctggagccgc
     1921 gctggcgatg tatcgcactg ctcatgctca aaacagaagg gctatcaaag cattgctgat
     1981 ttccggtgtg ctcacggtaa ttattggcgg cgtcagcgag ccgattgaat ttctgttctt
     2041 atttgtcagc ccgctgttgt ttgtcttcca tgcctttatg aatggattgg ctaacatggt
     2101 tctgccatat atgggcgtga agatgggctt tactggcgat ctgattcaat ttattagctt
     2161 tggtgtctta cgtggcacaa gaacgggatg gccattagcg gtatgtgttg aagtggctta
     2221 tttcttcatt tattactttg tgttccgttg gaccattctc aaatttaacc tgatgactgt
     2281 aggccgtgaa gagtccagtc ctgtcacgct gaacgctcac gaagaaactg caatagctaa
     2341 catcccagtt catgataaat cagagttgca ggcagcggag caaatggtta aggctctggg
     2401 tggtaaagaa aatattaaat cactggataa ttgtgtaact cgtttgcgtt taactatcgc
     2461 ggatatggga ttgatagatg aggctgcaat aaaaagggcc ggaggaattg cggttgtgaa
     2521 acttgatcaa aataccttac aagtcattat cggtacaaaa gtcatcgccc tacgtcggga
     2581 tatggataat tacatgggga tatgttgatg gatattttta atacctcaat ttctcgtaaa
     2641 ggaacatatt gcacacaatg ggacttttgt gaagacagat ttggtgtaaa agatgtatta
     2701 ccattctcta tttcagatat ggatcttccg ataccggaag ctatcatcag aacattaaaa
     2761 aaacgtctgg aacacccgat tttagggtac agtcgttggc agcatgatga ctatctgaat
     2821 tcgatagtca attggtatca tcaacaatat aaaacagaga ttcaaccaga atggatcacc
     2881 tatagcccaa gcgtgatgta ttcaatatct aaggcaatag aaatattgac attgaatgga
     2941 gataatattc tcattttcac gcctgtttat aacgcatttt ttgatgtgat taagcataat
     3001 ggtcgaaaca tgctaacaac ctctttgatg aaagaaaatg ggagaggcta cgttatcaac
     3061 tggcaagact ttgagcttaa aattaaaagc gcgaagatgg tgttattatg taatcctcat
     3121 aacccgacag gaacggtctg gtcggaagac gatcttcaca gaatcgcagc atgctgtgca
     3181 aggcataata tttggttatg ttcagatgaa atacatagtg attttgtttt taatcatagt
     3241 ttcacgtcag cgctaacaat aaaagatgac aaagtggtgg tgtttaattc gatttcgaaa
     3301 acatttaatg ttcctgcgct aaccggatca tatatgattt caactgacga taattttaat
     3361 cataagttca gaacgatatc gaggtatcgc gatttcgtaa actccccatc agtactgaat
     3421 gtaattgcga ccattgttgc ctataatgaa tgtgaagaat ggttaaagtc gttaaaggta
     3481 catattgcct ctaacattca atttaccttg cagtatttaa atgaaaatat tccggaattg
     3541 atcgtatggc cagctgaggg ctgctatttt tcgtgggttg attgctcggc ccttggttat
     3601 ccctttgata aattttaccg tcgattaatt aacgaaggga gagtaggtat tatggcgggg
     3661 catgtttatg gtactgaagg agagggctat ctgcgtctta atgtggcctg cagtcgagaa
     3721 aaattatata tggggcttgc gcgccttgcg agtgtaatta aaaatattaa tcaggaaaaa
     3781 gaaaatgaac aatacggatc ttatccatct tattaaacgt tttatgcgta acgaacataa
     3841 agcagtagaa gaggttattg attcccccct ttcggaagtc gcgaatttaa ttaaaatatt
     3901 acaatcttgc cagggaaaag ttgtttttat tggtgttggg aaatctggca ttattgccag
     3961 aaaacttgct gcaacatttg ccagcaccgg aactccctcg ttttttgttc acggtacgga
     4021 agcggtacac ggcgaccttg ggatggtggc gaaagacgat gttgttattc ttatttcaaa
     4081 cagtggtgag acggcggaaa tcctggcgac actgcctagt ttgaaaaaaa tgggtaatta
     4141 tttgatctct tttacccgca gtcatcactc atcgcttgcg ataagttgtg atttatctgt
     4201 tgaaatcccc gtcaaaagtg aggcggataa tttaggttta gcaccgtcat gctcgtctac
     4261 tgttgttctg gttgttggtg atgcggtggc gcttgcactc tctgaattga aaaaatttac
     4321 tcgtgctgat ttcggcttat atcatccagg aggtgcactc ggcattaaag caaattcata
     4381 atttcaaagt ataaatacag taaccggtca cgccatcttt tggggtggcg ttcattgaag
     4441 tgagtgtcag ctatgaaaaa tgttctggtc accgtcccca gcttttcagc acgctgtgtt
     4501 tccgccagca agttgctaag agagaataat tttaatctta ttattaagaa taatgtggag
     4561 catctgctta aatcagaatc cactgcatta cgagaatcta tttgcgcggt catcgccgga
     4621 aaagattgtt atcaggcgga cacgctatca ttgctgcctg gtgtgaaaat aatatcgaga
     4681 ttcgggacgg ggatagataa tattgatctt cgtgcagcac aacaatccgg tattgtagtc
     4741 aataatgcgg ttgggattaa ttcaaatgct gtcgcggaat ttatcattgg acttatcttc
     4801 gcgagcatga gaaatattcc aggcagctat catgcgatgc aaaatggcta ctggggtgag
     4861 tcgcatggct atgaattaca aggcaaacgg attggcatgg ttggctacgg caatataggt
     4921 aaaaccctgg caaaaaggtt atccggtttt gatgttgagc ttttagctta tgacaaacaa
     4981 cccgattatc aggttgctga caaagttgga gttcagtttg tatcaattga agatatcttt
     5041 atgcagtcgc atgtcatcat tgttcttttg cctttttctc ctgagctgga gaacttcatt
     5101 agtcataaat atttgtcgat gatgcgcaat ggtgctctaa tcatcaatgc cgcaagggga
     5161 aagttgctgg atgagggcgc tttactacag gtgattgaag aacgaaatgt gtttgcggcg
     5221 ctggatgtgt ttagctctga accgttggcg caatttagcc cactgctaca tgccaaaaat
     5281 atcattacaa cgccgcatat tgcggccgcc accgttgagt cataccagca aaccggtaca
     5341 cacgttgccc agtcgataat tgattacttc gcaggaaggg agataaaaaa cgtgttgtga
     5401 gttcagcgaa cgaagttttt aaatacaaaa aaaaccgcaa tttgtggaac acattgcggt
     5461 ttatcagcat tctaagtatt ggttgcgggg gccggatttg aaccgacgac cttcgggtta
     5521 tgagcccgac gagctaccag gctgctccac cccgcgtccg tggatgcgca ctatactccg
     5581 ctcgcgtttt catgcaacct tttttttacc ccgatcacgg atttagcatg attttgaaca
     5641 aaaacaaacc actaaagcct attttttgtg caaaatttgc gtcagggaga ggctgctcat
     5701 ttcattaaac gcggcggttt gttatcttcg ttgcgcctta ttttttaacc tgaagaagag
     5761 aacaatgaaa ggacgttggg taaagtacct tcttatgggc acggttgtgg caatgcttgc
     5821 cgcctgctct tccaaaccaa ccgatcgcgg acagcaatat aaagacggga aatttaccca
     5881 gcctttctct ctggtgaacc agccagatgc cgttggcgcg ccgattaacg ccggtgattt
     5941 tgccgagcaa attaaccata tccgtaattc gtcaccgcgt ctgtatggca accagagtaa
     6001 tgtttataac gcggtgcaag agtggctgcg cgcaggcggt gatacccgca atatgcgcca
     6061 gttcggcatt gatgcctggc agatggaagg tgccgacaac tatggtaacg tgcagtttac
     6121 aggctattac acgccggtaa ttcaggcgcg ccatacccgc cagggcgagt tccagtatcc
     6181 tatttaccgt atgccgccaa aacgtggtcg tctgccgtct cgtgcggaga tctacgcagg
     6241 ggcgctgagt gataaatata ttctcgctta cagcaactcc ctgatggata acttcattat
     6301 ggatgtgcag ggtagcgggt atatcgactt tggtgatggc agtccgctta actttttcag
     6361 ctatgcaggg aaaaacggtc atgcctatcg cagcattggt aaggtgctga tcgaccgtgg
     6421 cgaagtgaaa aaagaagata tgtcgatgca ggcgattcgt cattggggcg aaacacacag
     6481 tgaagccgag gttcgcgagc tgctggaaca gaacccgtct ttcgttttct ttaaaccgca
     6541 atcttttgca ccggtgaaag gggcaagtgc ggtgccgctg gttggccgtg cttctgttgc
     6601 ctccgatcgt tcaattattc cgccaggtac taccttgctg gcagaagtgc cgctgctgga
     6661 taataacggc aaatttaacg gtcagtacga attgcgtctg atggtggcgc tggatgtcgg
     6721 tggcgcaatc aaaggccaac acttcgatat ctatcaaggg atcgggccgg aagccggaca
     6781 tcgcgcaggt tggtacaacc actatggacg tgtctgggtg ctgaaaaccg ccccgggcgc
     6841 aggtaacgtc tttagcggct gatgtgttat tctgtatacc gtttttacgg atacgacagg
     6901 gtgagggata acctcaccct ttttaattct gaggtgctat gtctgtggta attagtgatg
     6961 catggcgtca gcgttttggt ggcacagcgc gtctgtatgg tgaaaaagcg ttgcaactgt
     7021 ttgctgacgc gcatatttgt gtggttggta tcggtggtgt cggttcctgg gcggcggaag
     7081 cgctggcgcg cacggggatt ggcgcaatca cgcttatcga tatggatgat gtgtgcgtca
     7141 ccaataccaa tcggcaaatt cacgctctgc gcgataacgt cgggctggca aaagcggaag
     7201 tcatggcgga gcgtattcgc cagattaacc cggagtgccg tgtaacggtg gtggatgatt
     7261 tcgtgacgcc ggataacgta gcgcagtata tgagcgtggg gtattcgtac gtgattgatg
     7321 ccattgatag tgtacggccc aaagcggcgc tgattgctta ttgtcggcgc aataaaatcc
     7381 cgctggtcac gaccggtggc gcgggtgggc agattgaccc gacgcagatt caggttaccg
     7441 atctggcgaa aacgattcag gatccgctgg cggcgaagtt gcgcgagcgc ctgaaaagcg
     7501 attttggcgt agtgaaaaac agtaaaggta agctcggcgt cgattgcgtg ttttctactg
     7561 aagcgctggt gtacccgcag tcagacggta ctgtgtgtgc gatgaaagcc acggcagaag
     7621 ggccgaagcg gatggattgt gcatctggat ttggcgcggc aacgatggtg accgccacct
     7681 ttgggtttgt tgcggtttct catgcgctga agaagatgat ggcgaaagcg gcgcgtcagg
     7741 gttaaacaat caggtcggat gcgacgcgaa cgtcttatcc gacctggttc tgtgcttaaa
     7801 cctgcttcgt cgcagcgata atcgcctcgc ttaacgcatt caacccctgg ctgcgtgagg
     7861 cgctaagctg cccacgcaat cccagctcat caaacaatgc cagcggcgac tgtgcctgca
     7921 attcggcggc ggttttcccc tcaacggcag tcaacagcac cgccagcagg ccgcgcacta
     7981 tacgtccttc gctgtcgcca aagaagtgca ttttaccgtt ttcagccacg gtatatccca
     8041 gccagacgcg gttttcgcat ccggcaatct ctttagcctg cgcttttaac tcgtctggca
     8101 aagccggaag ctgtttcccc agcatgatca actggcgata tttatcttcc cattgcgtca
     8161 acggtgcgaa ggtattgcgt aacgtttctg cggttacggt tgtgccgaac ggatgtccgg
     8221 cgaattgcgg gttcgtcatt aatccaccaa taattccagc gcgcggtcaa cggcattcac
     8281 cagcgcatcc acatcactct ttgtattata tggcgcaaaa gaggcgcgca gtgtgccggt
     8341 tacgcctaat tctgccagta gcggctgagc gcaatgttgc ccggcccgca aggcaatacc
     8401 gtactccgcc agcagcgtca ccatatcgct gtgatgaacg ccagcaaaat caaaggccag
     8461 caggctggaa tcctggcagc ggaatgaacg gaaacctgga cgtttcgcca gcgcatcttc
     8521 cgccagtgtt gctaagctac ggctccagtt ttcggcctgg ttgatatcgt aatctgccag
     8581 ccattccagc gccgcgctta atcctatgac accagccaca tttggcgttc ctgcttccag
     8641 tttccacggc gcagattgag tcgtgaagcc gtcaaaactc acttcgtgaa ccattttgcc
     8701 gccgcccagc cagggcgaca tcgcttccag cagttctgat ttgccataca gcacgccgat
     8761 acctgtcggg ccatacagtt tgtgacctga aaaagcatag aaatcaatat tcagttgctg
     8821 aacatccgcg gggaaatgca ccgccccctg agcaccatca accatcacca ccatcccggc
     8881 tgaatgagca aaggtaatcg ctcgcgccag atccgggcaa ccgccagtaa cgttcgacat
     8941 ctgacccaac gccagaatcc gactacgggg agtaatcagt tctggcaaca aatcgacatc
     9001 cggcagtcgc tgcgcattaa gcggcaattt caccactttg gctccagttt gttgggcgac
     9061 catcagccag gggacgaggt tggcgtggtg ttctgccacg ctgacaataa tctcatcgcc
     9121 cagttgcaga cgcggacgcg catagcattg tgcaaccatg ttgatggatt cagtggtgcc
     9181 gcgcgtccag acgatagttt tatcatccgg tgcattcagt aattgcgcca ctttctctcg
     9241 tgcagcttca taacgcgcgg tcaggcgttg ggcttcggca aactggctgc gatggacgtt
     9301 tccggcgctc aggctgtaaa actgtcgggt ggcttcaacc acggcttcag gtttaagcgc
     9361 ggtcgcggcg ctgtcgagat agacgcccgc atcctgtaga gcgggaaact gggcgcgaaa
     9421 ctgcgcggga ttaaaaacgt tcatggtact cctcggcttg atgcaccgat cgtgacgcaa
     9481 aattcacttt gatacaagga gagtgctaac catcagaatt atctggatat cctggcgaag
     9541 ccgcgtaagt aacatatgct gaatagtgtt aggcgttaat tttagcctgt taatcaatac
     9601 tattaagatt tattaatcgc tttaaggaga agatgatgaa aaagactgcc gcaattattt
     9661 ctgcctgtat gctgactttt gccctgagcg cctgttccgg ttcgaactat gtgatgcaca
     9721 ccaatgacgg acgtaccatc gtctctgacg gcaaaccaca gactgataac gataccggta
     9781 tgatttcgta taaagacgct aatggcaaca aacagcagat caaccgtact gacgtgaaag
     9841 agatggtcga actggatcag taattcacga tcgcaaggta aaaaaaagca ccgcaattag
     9901 gcggtgctac attaatcact atggacagac agggtaaatg tacaggaagt gaaaaaaggt
     9961 agctttgcta ccatggtctg aatcgcagac caattgcaaa cacaacaaca caacatcaca
    10021 accgtaagcc aaaagttcac cagaacacgc attccgataa aacttttcgt tccggctcag
    10081 gaagtgccgc cactataggt atttgctggt agaagctcaa cggacaattt ataatggctc
    10141 agattaaaaa aacta
//
LOCUS       DAZKGS010000155        10124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKGS010000155 DAZKGS010000000
VERSION     DAZKGS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            207..1580
                     /gene="aroP"
                     /locus_tag="ACHOQY_003908"
     CDS             207..1580
                     /gene="aroP"
                     /locus_tag="ACHOQY_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9679913.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1623..2477)
                     /gene="ampE"
                     /locus_tag="ACHOQY_003909"
     CDS             complement(1623..2477)
                     /gene="ampE"
                     /locus_tag="ACHOQY_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9679914.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2474..3025)
                     /gene="ampD"
                     /locus_tag="ACHOQY_003910"
     CDS             complement(2474..3025)
                     /gene="ampD"
                     /locus_tag="ACHOQY_003910"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9679915.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3113..4006
                     /gene="nadC"
                     /locus_tag="ACHOQY_003911"
     CDS             3113..4006
                     /gene="nadC"
                     /locus_tag="ACHOQY_003911"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9679916.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4209..4649
                     /gene="ppdD"
                     /locus_tag="ACHOQY_003912"
     CDS             4209..4649
                     /gene="ppdD"
                     /locus_tag="ACHOQY_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9679917.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4659..6044
                     /gene="gspE"
                     /locus_tag="ACHOQY_003913"
     CDS             4659..6044
                     /gene="gspE"
                     /locus_tag="ACHOQY_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9679918.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6034..7236
                     /gene="hofC"
                     /locus_tag="ACHOQY_003914"
     CDS             6034..7236
                     /gene="hofC"
                     /locus_tag="ACHOQY_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9679919.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7271..8314)
                     /gene="guaC"
                     /locus_tag="ACHOQY_003915"
     CDS             complement(7271..8314)
                     /gene="guaC"
                     /locus_tag="ACHOQY_003915"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9679920.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8470..8514)
                     /locus_tag="ACHOQY_003916"
     CDS             complement(8470..8514)
                     /locus_tag="ACHOQY_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679921.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8539..9159
                     /gene="coaE"
                     /locus_tag="ACHOQY_003917"
     CDS             8539..9159
                     /gene="coaE"
                     /locus_tag="ACHOQY_003917"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9679922.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9159..9902
                     /gene="zapD"
                     /locus_tag="ACHOQY_003918"
     CDS             9159..9902
                     /gene="zapD"
                     /locus_tag="ACHOQY_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9679923.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9912..10109
                     /gene="yacG"
                     /locus_tag="ACHOQY_003919"
     CDS             9912..10109
                     /gene="yacG"
                     /locus_tag="ACHOQY_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9679924.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2486 a         2666 c         2594 g         2378 t
ORIGIN      
        1 aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc
       61 tcataccatt attcttgatc tgacggaagt ctttttgtaa caattcaaac ttctttgatg
      121 taaacaaatt aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca
      181 aaaaaacaac caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa
      241 agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg
      301 ggttattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt
      361 acgccattgc tggttttatc gcctttctga tcatgcgtca gctgggtgaa atggtggtcg
      421 aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg
      481 gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga
      541 ctgccgtggg taaatacatt cagttctggt atccggaaat ccccacctgg gtttctgccg
      601 ccgtattctt tgtggtgatt aacgccatca acctgaccaa cgttaaagtg tttggcgaga
      661 tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg
      721 gctggctgct attcagtggc aacggcggcc cgcaggcgac cgttagcaac ctgtgggatc
      781 agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg atgatcatgt
      841 tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc
      901 aaagtatacc gaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt
      961 cgttagccgt tctgctctca ctgatgccgt ggacccgcgt taccgccgat accagtccgt
     1021 ttgtgctgat cttccacgag ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg
     1081 tactgactgc ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt
     1141 ttggtctggc acaacagggt aatgcgccaa aagcgctggc gtctgtcgat aaacgtggtg
     1201 taccagtaaa taccattctg gtgtctgcac tggtaacggc gttgtgcgta ctgattaact
     1261 accttgcccc agagtccgct ttcggactgt taatggcgct ggtggtatct gcactggtaa
     1321 tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag
     1381 gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt
     1441 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
     1501 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
     1561 aagccgtaaa agcgcattaa tctctctacg ccctcacccg tacagggtga gggcaataat
     1621 ctttacacca acgccccgta aatcgtcagt agtgcaatca ccaccacgac cacgaacgag
     1681 gttttcttcg ccattgaaac cgctgccctc ggcgtctcca ccttatcgac atgcggttca
     1741 cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaaatca
     1801 cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa ggcatatacc
     1861 acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac
     1921 tgtaaacgat gatgcggcgt ctgatatcgc gccagccagt attgccatgc acgcaaaaac
     1981 gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt
     2041 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
     2101 cgttcgtcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca
     2161 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
     2221 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagtagcgt gggaacgttg
     2281 aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
     2341 atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
     2401 tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
     2461 gttgtaaata gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt gcccaatcaa
     2521 atgcaggacc gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgtttt
     2581 tagcgatatc cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact
     2641 gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat
     2701 tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag
     2761 gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat
     2821 gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt
     2881 cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg
     2941 tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa
     3001 ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt
     3061 tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc
     3121 tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat
     3181 ccccggcgcg gtagcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa
     3241 tgatattacg gcaaaacttt taccggaaaa ttctcgctct catgccacgg tgatcacccg
     3301 cgagaatggc gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg
     3361 cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaatg ccaatcaatc
     3421 cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa
     3481 ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg aattgctgga
     3541 aggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagctct
     3601 gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt
     3661 cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc
     3721 gtcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga
     3781 tgaagccctg aaagcaggag ccgatatcat catgctggat aacttcgaaa cagaacagat
     3841 gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac
     3901 tgacaaaaca ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct
     3961 aactaaacac gtacaagcac tcgacctttc aatgcgtttt cgctaatagt tgacagattt
     4021 atccgctcca tcgcgggcgg ataatctccc cttccccaac tttcttcgta acgcctcgca
     4081 aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg aatctgcttt cccgtttcgt
     4141 tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacta aatcaaggag
     4201 cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca
     4261 tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg
     4321 cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc
     4381 tggaacatgg tggattagat acctgcgacg gtggcagcaa tggcattccc tcgcctacca
     4441 ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag
     4501 aaagtctcaa tgggctaagc gtcgtcatga caccgggttg ggataacgca aacggcgtca
     4561 ccggctggac gcgcaactgc aatattcaaa gtgacagcgc attgcatcaa gcctgcgaag
     4621 atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcactg
     4681 ccctgtgtct gcgttatcat ggagtcttgc tggatgccag cgaagaggtg gttcatgttg
     4741 cggtagtcga tgcaccttcg catgagctac tggacgcatt gcatttcgct accaccaaac
     4801 gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac
     4861 agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg ctaactcgaa
     4921 cgttacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg
     4981 cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggat gtttcaccgg
     5041 atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat attgcggaac
     5101 atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc
     5161 gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg
     5221 tgagccaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg gcggactttg
     5281 ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctaca ggcagcggca
     5341 aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta
     5401 gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc
     5461 gtgccggact cacctttcag ggcgtgttgc gtgcgttatt gcgccaggat cctgacgtca
     5521 tcatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa gcggcgcaaa
     5581 ctggtcacct ggtgttgtct accctacaca ctaattccac ctgcgaaacg ctggtacgtt
     5641 tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg gtaatagccc
     5701 agcgtctggt acgcaaactt tgcccacatt gtcgccggca gcaaggggag cccatccaca
     5761 ttccagacaa tgtatggcca tcgccgctgc cccactggca ggcacccggt tgtgtacatt
     5821 gctaccacgg tttttatggt cgtacggcct tatttgaagt tctgcccata acgccggtca
     5881 ttcgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac gcacgacagg
     5941 cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct
     6001 ttgaagagtt aatccgcgta ctggggatgc cgcatggcga gtaagcaact ctggcgctgg
     6061 catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtgct
     6121 ttgctgctca tggcactaca gcaacagatg gttaccccac ttagcctgaa gcgaatcgcc
     6181 atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg
     6241 ctactcaaag ccgggttaac gctttctgaa gggctggcac tgctggcgga acagcatccc
     6301 agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct
     6361 ttttccaatg ccttattacc ctggtcagag gtatttccgc cactctatca ggcgatgatc
     6421 cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa
     6481 aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc catcatcatt
     6541 ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc
     6601 gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat catgacgctg
     6661 gcagacttta gtggcgaatg gagctggctg ctggtgttgt tcggctttct gctggcgata
     6721 gccaataagt tgctgatgcg ccgaccgacc tggcttatag tgcggcagaa attgctgtta
     6781 cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ttttacgatt
     6841 ctggcgctga cacaaagtgc aggcattact tttttgcagg gcgtagagag cgtcagagaa
     6901 acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac
     6961 ggtcatccca tctggctggc gctaaaaaat accggggagt ttagcccgct ctgtttgcaa
     7021 ttagtgagaa caggagaggc atccggctcg ctggacctca tgttagacaa cctcgcccat
     7081 catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg
     7141 ttgctgatca taacgggagg aattatcggt acgctggtgg tggcgatgta tctgccaatt
     7201 ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc tccacaccag
     7261 cgttggaaga ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca cggataaatg
     7321 tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca
     7381 ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg
     7441 ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc atcgcagact
     7501 cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc
     7561 cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa
     7621 aggcttttgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc attccgccca
     7681 gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa ccgacgcctg
     7741 ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcagcac
     7801 cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt
     7861 tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat
     7921 tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt
     7981 tttcgaaatc cgcatcagac gtaccggtag aaaccatcac atgtttcagc acatcagcgg
     8041 aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca
     8101 aaatatcaaa agaagccagc gcagaggcca tagaaaatgt acctacggtg tccatatttg
     8161 cggcgataat tggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt
     8221 ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt
     8281 taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta
     8341 taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga
     8401 ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg
     8461 aacgtttcat caatttcgct ttcgtgcact catttttaac gttttgataa tcatgatgaa
     8521 ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc
     8581 aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt
     8641 attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt
     8701 ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc
     8761 ttcgccaacc cggaagagaa aaactggctt aacgccctgc tgcatccgct gattcagcaa
     8821 gagacgcaac accagatcca gcaagctact tccccctatg tactgtgggt tgtgccattg
     8881 ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca
     8941 gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa
     9001 atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga cgtcattgat
     9061 aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg
     9121 cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg
     9181 aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac
     9241 tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca
     9301 gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaacttg
     9361 accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta
     9421 ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta
     9481 tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc
     9541 caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg
     9601 cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac
     9661 ttaccatggt gctggattta attcgccagt cggctccctt ccgtaaacaa accagcctga
     9721 atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg
     9781 attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt cgttttatgc
     9841 cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct
     9901 aaggagtgaa aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg
     9961 tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc
    10021 tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg
    10081 atgactggag cgaagagcca aagcagtgac atttgtctga tgcc
//
LOCUS       DAZKGS010000156         9992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKGS010000156 DAZKGS010000000
VERSION     DAZKGS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..642)
                     /gene="yncG"
                     /locus_tag="ACHOQY_003920"
     CDS             complement(25..642)
                     /gene="yncG"
                     /locus_tag="ACHOQY_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP9679925.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            908..2407
                     /gene="ansP"
                     /locus_tag="ACHOQY_003921"
     CDS             908..2407
                     /gene="ansP"
                     /locus_tag="ACHOQY_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9679926.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2522..3583)
                     /gene="yncE"
                     /locus_tag="ACHOQY_003922"
     CDS             complement(2522..3583)
                     /gene="yncE"
                     /locus_tag="ACHOQY_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9679927.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3825..5927
                     /gene="pqqU"
                     /locus_tag="ACHOQY_003923"
     CDS             3825..5927
                     /gene="pqqU"
                     /locus_tag="ACHOQY_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9679928.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5963..6628)
                     /gene="mcbR"
                     /locus_tag="ACHOQY_003924"
     CDS             complement(5963..6628)
                     /gene="mcbR"
                     /locus_tag="ACHOQY_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP9679929.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6826..7863)
                     /gene="curA"
                     /locus_tag="ACHOQY_003925"
     CDS             complement(6826..7863)
                     /gene="curA"
                     /locus_tag="ACHOQY_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9679930.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8044..8562
                     /gene="mddA"
                     /locus_tag="ACHOQY_003926"
     CDS             8044..8562
                     /gene="mddA"
                     /locus_tag="ACHOQY_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9679931.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8559..9008
                     /gene="ydcZ"
                     /locus_tag="ACHOQY_003927"
     CDS             8559..9008
                     /gene="ydcZ"
                     /locus_tag="ACHOQY_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9679932.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGALMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9009..9242)
                     /gene="ydcY"
                     /locus_tag="ACHOQY_003928"
     CDS             complement(9009..9242)
                     /gene="ydcY"
                     /locus_tag="ACHOQY_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9679933.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9328..9501)
                     /gene="ortT"
                     /locus_tag="ACHOQY_003929"
     CDS             complement(9328..9501)
                     /gene="ortT"
                     /locus_tag="ACHOQY_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9679934.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9696..9791
                     /gene="yncL"
                     /locus_tag="ACHOQY_003930"
     CDS             9696..9791
                     /gene="yncL"
                     /locus_tag="ACHOQY_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9679935.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2402 a         2447 c         2440 g         2703 t
ORIGIN      
        1 taacataatc ttctggtatc ccactcaaat aatttcattc ctttttaata cttcttgtaa
       61 tttcggtaac tgacatacag catcagcgat cgcgctgata ttctgcgcat tatcctgaaa
      121 ccattcatgc cccggccccc atgtgcgcat ggtgcagaga taacagtcca ccagcgttag
      181 ttgctcacca aaggcataag gttcagcggt caattgtgaa ttcagccaga tataaagtga
      241 tttgcgatat tcaattacat tcttctttaa ctgttcaggt gcgtcagatg cccaacgctc
      301 agggtaatcg gcgaaagtga atgttggata gacattagca accagccaga ccaatagccg
      361 ttgaaactgc tgacgttcgg cgcgcccaac cggtggcgca aggtccgggc gacgatcgag
      421 aaccataaat gcaatcgccg ctgtctccgt cataatctcg tcattttcca gcgccagtgt
      481 cggaacctga cacaacgggt tcagggtttt caataactcg cgtgaagctc cctcgtggtc
      541 aaaaccactg acatcaacga actgataagg aatatcagcc agtgttagca tcagctcact
      601 gattgtagag ccccatccgg gtacgccata caccttaatc atatgccctc acaatctttt
      661 aaagattaag tgtaggacag tacacaggtt aaccagcaaa aagatttgtc attttttcgt
      721 cagttgtatt attccccttc aggattgtaa ggggatgttt tgcatcgttt atcgataacg
      781 ggtaggatgc tgcgccgata gttttatacc cataaagaat aatggtgata actatcatcg
      841 ccaggatgaa taaacattgt tcatggcaac ttatatgact ttttcattaa agcaatcagg
      901 gagagcaatg agtaaacacg acaccgacac ttcagatcaa cacgccgcga aacgccgctg
      961 gcttaatgcc cacgaagagg ggtatcacaa agcgatgggc aatcgccagg tacagatgat
     1021 cgccattggc ggcgcgattg gcaccggctt gtttttaggt gcaggagccc gactgcaaat
     1081 ggcgggtcca gcattggcac tggtttattt aatttgtggc ttgttttcgt tttttattct
     1141 gcgtgcattg ggtgagctgg tgctacaccg cccttccagt ggcagttttg tctcttatgc
     1201 ccgtgagttt ttgggtgaga aagccgctta tgttgctggc tggatgtact tcatcaactg
     1261 ggcgatgacg gggattgttg atattaccgc cgtcgctctg tatatgcatt actggggtgc
     1321 gtttggcggc gtgccgcagt gggtctttgc gctcgctgca cttaccatcg ttggcaccat
     1381 gaatatgatc ggtgtgaaat ggtttgcgga gatggagttc tggtttgcgc ttattaaagt
     1441 gctcgccatt gtgacctttt tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct
     1501 ggatggcaac accactggct ttcatttaat taccgataat ggcggcttct tcccccacgg
     1561 tttgctgcct gctctggtgt tgattcaggg cgtagtgttt gcttttgcct ccattgaaat
     1621 ggtgggtaca gctgccggag aatgtaaaga tccgcagacc atggtgccta aagcgattaa
     1681 cagcgtgatt tggcgtattg gcctgtttta cgtcggctcc gtggtgttgc tggttatgct
     1741 attgccgtgg agcgcgtatc aggcggggca aagtccgttc gtgacgtttt tctctaaact
     1801 gggtgtgcca tatatcggca gcattatgaa cattgtggtg ctgaccgctg ccctctccag
     1861 cctgaactca ggtctgtact gcaccggacg tattctgcgc tcaatggcga tgggcggttc
     1921 cgcaccgagt tttatggcga aaatgagtcg tcagcatgtg ccgtatgccg ggattctggc
     1981 gacactagtt gtgtatgtcg tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt
     2041 tgagattgtg ttgaacttcg cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt
     2101 gtgccagatg cgcctgcgta aagcaattaa acaaggcaaa gcagcggatg tcagttttaa
     2161 actgcctggc gcgcccttca cttcctggct gacattactg tttttactga gtgtcctggt
     2221 gctgatggcg ttcgattacc cgaacgggac ttatactatc gcggcgctgc cgattatcgg
     2281 tattctgctg gttataggct ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac
     2341 tgcgccagtc gtcgaggaag atgaagaaaa acaggaaatt gtgtttaagc ctgaaacggc
     2401 gagctaattg caaaattgcc cggctacgtg ccgggcaaat ttagattact gacgaaggtg
     2461 tgccgcatgc ggcgtgataa aagtcaggca agtctctccg ttttcaccct cgccacatct
     2521 attacagcgc aatacgaatc acatcgtccg gctgggtagc ttcctgctgt ttagtggatt
     2581 tttgtttcac actgacatac agcgttttgc catcggcaga cagcgccagg ctgtttggat
     2641 gagtcggcgt atcgaacgtt ttcaccactt tatagctttt cgcgtcaatc acactgactt
     2701 tacctgcctg acgatgcgtt acgtaggctt cattacgcgc tgggttaaac agcacagcca
     2761 gtgattctgg tgccgcaacc ttcgccagaa tattgccatt acgggtatcg accactaaca
     2821 cttctgcggc tttagaatcg gtgataaatg cacgctgcct ggcggtatca aggctaatgt
     2881 tgataaagaa gtgctctttg ccgtcatcca gcagcttttt acggctgagg attttattgt
     2941 cggcggtgtc gatggtaatc aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt
     3001 tgctatccag cgccagaccg gtactcattt taccggtgtt ctggatggcg gttttcagtt
     3061 tgatattctc gccatcaacg acccaaatca cgctctcttt accaatacca ctgatgtaaa
     3121 cggtgttcgt ggcatcatca gctaccagct cacgcggttg cagcgggcgc acctcttccg
     3181 tgcgcttacg atcatccagc accagacggc ctttcacctc gcccgttttg gcatctatcg
     3241 ccgtgaccgc gctgtttacg gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg
     3301 tggcaccaaa cggcttgaga tcgttatgga tcgcctgcgt cacttccaga gtgaccggat
     3361 caagacgata aaccacgcca cctttatcca gtttgcggct ttgcgaagtg gcgagccaca
     3421 gtgcgttttc ttgctggcta taagccattt cgtaggcacc tttacctacc gctttacgca
     3481 gcatttcttc tgcggcctgc gtactgaatg atgaagcaac aagcaatgaa cctaacagta
     3541 atgaaccacg caggcgcgat gaaaacagat gacgtaaatg catgacgact ccctttgatg
     3601 aaccgatatt tttgctgctt cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt
     3661 tataaatggg aatggtaatc attattttct caaatgtgga gcatttcttg ccgttacgac
     3721 tacaattttc tcaattactt aacgtatcgt gccgtttcgt tttccataaa tttacaattt
     3781 cattaccata acggcgtttt cccattctta acctggcttt tttcatgaag attttttccg
     3841 tccgacagac cgttttgccc gcactgcttg tcctttcccc cgttgttttt gccgctgatg
     3901 aacagactat gattgtcagt gccgcaccgc aggtggtttc agaactggat accccagcag
     3961 cagtaagcgt ggtggatggc gaggagatgc gcctggcaac accgcgcatt aacttgtccg
     4021 aatcactgac cggcgtgcct ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt
     4081 tacagctgtc gattcgcgga tttggctccc gctccactta cggtattcgc ggtattcgcc
     4141 tgtatgtgga cggtattccc gccaccatgc ccgacgggca agggcaaaca tccaacatcg
     4201 atttaagcag tgtgcaaaat gtggaagtgc tgcgtggccc cttctctgcc ctgtatggca
     4261 acgcgtctgg tggggtaatg aatgtcacca cccagaccgg acaacagcca ccaaccattg
     4321 aagccagtag ttactacggc agttttggca gctggcgcta tgggctgaaa gcaacgggcg
     4381 caacgggaga cggcacacag cctggcgatg tcgattacac cgtctcaacc acgcgtttta
     4441 cgacccacgg ctatcgtgac catagtggcg cacagaaaaa tttagctaat gccaaactgg
     4501 gcgtacgcat tgatgaagcc agcaaattaa gtctgatttt caatagtgtg gatatcaaag
     4561 cagatgaccc aggtgggcta accaaagcag aatggaaggc taatccacaa caagcgcctc
     4621 gtgcagaaca gtacgacacg cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg
     4681 agcgtagcct gagcgcgcaa gatgatatga gtgtgatgat gtatgccgga gagcgagaaa
     4741 cgacccagta ccagtcaatc ccgatggccc cccaacttaa cccgtcacat gcgggcggcg
     4801 tgattaccct gcaacgccat taccagggga tagacagccg ctggacacac cgtggtgaac
     4861 tgggcgttcc ggtcacgttc actaccggcc tgaactacga aaacatgagt gaaaaccgca
     4921 agggctacaa taacttccgc ctgaatagcg gcatgccgga gtacgggcaa aaaggtgagt
     4981 tgcgtcgcga cgaacgcaat ctgatgtgga acatcgatcc ctatttacag acgcagtggc
     5041 agctgagcga aaaactgtcg ctggatgctg gcgtgcgcta cagctccgtg tggtttgatt
     5101 ccaacgacca ttacgttact ccgggtaacg gcgatgacag cggtgatgcc agttatcata
     5161 aatggctacc tgccggttcg ttaaaatatg caatgaccga tgcctggaat atctatctgg
     5221 cagccgggcg aggttttgaa acgccgacga ttaatgagct gtcttatcgt gctgatgggc
     5281 aaagcggtat gaactttggt ttaaaaccat ccaccaacga tacaattgag atcggcagta
     5341 aaacgcgtat tggtgatggg ctgcttagtc tcgcattgtt tcagaccgac actgatgatg
     5401 aaattgttgt cgatagcagt agcggtgggc gtacgactta caaaaatgcc ggaaagaccc
     5461 gtcgtcaagg cgctgaactg gcatgggatc aacgtttcgc aggagatttt cgcgtaaacg
     5521 cgtcctggac ctggcttgat gcgacctatc gcagcaatgt ttgcaatgaa caggattgta
     5581 acggtaatcg gatgccaggg atcgcccgta atatgggctt tgcgtcgata ggttatgtac
     5641 cggaagatgg ttggtatgca ggcacggaag cgcgttatat gggcgatatt atggcagatg
     5701 atgaaaatac ggcaaaagcg ccgtcttata ctctcgtcgg cttattcacc gggtataaat
     5761 acaattacca caatttaact gtggatttat ttggtcgtgt cgataattta ttcgataaag
     5821 aatacgttgg ttctgtcatt gtcaatgagt caaacgggcg atattacgaa ccttcgcccg
     5881 gacgaaatta tggtgtcggc atgaatattg cgtggagatt tgagtaatta ccttgatgcc
     5941 cggtatttgc cgggcattta ctttaacgat tgtattgctg gtataaaata gcaatatttt
     6001 gttgcataat ttcagcaaga caatgtctgc atccctcttt gtcttttgct tttaacgcag
     6061 cgagtaacag atgatagttt tctatatgct cccgtaattc cgctggatta atcgcttcat
     6121 agagataatg taaaccaggc cccatcctga cccacagttg ctcaatcatc tcacacagga
     6181 tgggcatatt tgagcgatga taaatcgcta agcgaaatag cctgtttaca ttaatgattt
     6241 gttccatgtc gcccttttct tgcgcctgct gtaatttctc gagcaattcc tggagttccg
     6301 ccaggtcttg cggggtgagg ttttcaacag ccagagcaac tgccattaat tccagttcgt
     6361 agcggatccg attgatttcg tctaattgac gtttcctcac ttccggaact gtaaatgctt
     6421 gtgcaggtgc gacagaaagc gcattcaccg aaaccagacg taataatgct tcacgcacag
     6481 gtgtaatact catacctaat tgctccgcca gatttttagt aatcaggcgt gcgccaggtt
     6541 ttaatgcgcc aatactaagc tgatgtttca ggtcgttctc aacctgcaga gtcaaactga
     6601 catgtttcat ttttcccatt ccaggcattt taattccttc gcgtgttttc tgtccaaccc
     6661 tgcctgcacg gcagaaggag ttcacctggt gtattaaagt gaataatcat aaaccaccca
     6721 cctttgttgt tattagcagc agaacaatga cgtggttagt taattaaagc tgaaaataag
     6781 ctttttgcct ggatttttat ggcggcttac gccgccgttg aaactttaat catcacccgc
     6841 gacgcggatc accactttgc cgaagttttt acccttcagc aggccgataa acgtctgtgg
     6901 cgcattctct aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca
     6961 ttgccccatc tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa
     7021 cccttgcaag cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg
     7081 accgggtggt agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc
     7141 agatgtatta agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata
     7201 gatatcaata ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg
     7261 atcaagacaa acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc
     7321 ggcaaccccc accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac
     7381 aggacctgtc gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag
     7441 cagccccata taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt
     7501 ttgcggatga tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca
     7561 tccactgtag cccagcaccc agtcgccagg ctgataatca ggatgattcg actccacaac
     7621 acggctcacc gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg
     7681 ctcatcgctc atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa
     7741 cacctgtcct tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac
     7801 cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgcct
     7861 cataatgctc tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg
     7921 gttttgtttg cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg
     7981 ttgcttcggg ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca
     8041 gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac
     8101 cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc
     8161 attgcctggt ttgaagcgcg gaccatagca ggttatccgg tgctggtgag tgaagaagat
     8221 ggcgtagtga cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat
     8281 accgtggaac attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa
     8341 ttgttaagcc gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg
     8401 atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccagt cgctgggatt tgtcgtcacc
     8461 gcacaaatgc cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag
     8521 ttgcaactcg acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg
     8581 cttttttgat tgccgccgga attggtctgg tagtccagaa cacgctgatg gtacgcatca
     8641 cccagacctc ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc
     8701 tctttgtttc tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca
     8761 gcgtgcgctg gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta
     8821 tcagcggata tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc
     8881 taattggcgc tctgatgctg gatatattcc gcagtcatgg cgtaccacta cgggcgttgt
     8941 tcggcccaat ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct
     9001 cattttaatc agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct
     9061 tctttatgct tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag
     9121 cgatcttccc gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg
     9181 gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac
     9241 atactttctc cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg
     9301 tggtgccgtc aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa
     9361 actcatcggc catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc
     9421 gtgagaaaga aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag
     9481 catatgttga tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat
     9541 atgcgtcatt ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac
     9601 agcaacttgc ccttatccat cttcagaatg agtattattg cggattttct taacccgtac
     9661 tatacacata ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag
     9721 tactgataaa tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct
     9781 ggtttatttg atgtagtgat tttgcaagca gcacgtactg tcagagaaat caactctctg
     9841 acagtacgca agatcttctc atactctcca ggtttacaac attatcctta atatttactt
     9901 ctttttgctg gcatctacat tgaaatgagt agaaaacatc cccctactgt cgtctataca
     9961 aacagcttac tgattcaaac ggaatcttgt tt
//
LOCUS       DAZKGS010000157         9901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKGS010000157 DAZKGS010000000
VERSION     DAZKGS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..553
                     /gene="osmC"
                     /locus_tag="ACHOQY_003931"
     CDS             122..553
                     /gene="osmC"
                     /locus_tag="ACHOQY_003931"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9679936.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(609..1535)
                     /locus_tag="ACHOQY_003932"
     CDS             complement(609..1535)
                     /locus_tag="ACHOQY_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9679937.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(1528..2514)
                     /locus_tag="ACHOQY_003933"
     CDS             complement(1528..2514)
                     /locus_tag="ACHOQY_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9679938.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2511..3407)
                     /gene="ddpC"
                     /locus_tag="ACHOQY_003934"
     CDS             complement(2511..3407)
                     /gene="ddpC"
                     /locus_tag="ACHOQY_003934"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP9679939.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3404..4426)
                     /locus_tag="ACHOQY_003935"
     CDS             complement(3404..4426)
                     /locus_tag="ACHOQY_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9679940.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4428..5978)
                     /locus_tag="ACHOQY_003936"
     CDS             complement(4428..5978)
                     /locus_tag="ACHOQY_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9679941.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5992..6573)
                     /gene="ddpX"
                     /locus_tag="ACHOQY_003937"
     CDS             complement(5992..6573)
                     /gene="ddpX"
                     /locus_tag="ACHOQY_003937"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP9679942.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(6831..9230)
                     /gene="dosP"
                     /locus_tag="ACHOQY_003938"
                     /gene_synonym="pdeO"
     CDS             complement(6831..9230)
                     /gene="dosP"
                     /locus_tag="ACHOQY_003938"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP9679943.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9255..>9901)
                     /gene="dosC"
                     /locus_tag="ACHOQY_003939"
     CDS             complement(9255..>9901)
                     /gene="dosC"
                     /locus_tag="ACHOQY_003939"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP9679944.1"
                     /translation="GIFNQTMRNTRNLNNRSLRVKFLLQIRNTVSQIITLLRELFEEV
                     SRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDVDKFKEINDTWGHNT
                     GDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLRTAERIRSRVEKTKL
                     KAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGRNRVELWKASL"
BASE COUNT         2435 a         2579 c         2498 g         2389 t
ORIGIN      
        1 aatttaattt aagtgcagaa gtaatatttc tgccggattt ttattcggaa tatcctgctt
       61 atcctcgtgc tgtttctcac gtagtctata atttcctttt taagcccaca ggagagcaac
      121 aatgacaatc cataagaaag gtcaggcaca ctgggaaggc gatatcaaac gcgggaaggg
      181 aacagtatcc accgagagtg gtgtgctgaa ccaacagccg tatggattta acacgcgttt
      241 tgaaggcgaa aaaggaacta accctgaaga actgattggc gcagcgcatg ccgcatgttt
      301 ctcaatggcg ctttcattaa tgctggggga agcgggattt acgccaacat cgattgatac
      361 caccgccgat gtgtcgctgg ataaagtgga tgccggtttt gcgattacga aaatcgcact
      421 gaagagtgaa gttgcggtgc cgggtattga tgcctctacc tttgacggca tcatccagaa
      481 agcaaaagca ggatgcccgg tttctcaggt actgaaagcg gaaattacgc ttgattacca
      541 gttgaaatcg taaagcattg ccggatgacg cgtcaggcgc gtgaatgcct gatgcgttgt
      601 tagcatctct acagcgcccg ccagcaccgg atctcacgtc cgtcctccct tattgttaat
      661 gattgccgga cttcacatcc gtgggttgcc agagggcaac gttcgtagaa aaaacagcct
      721 tgcggcaacg tgcggtttcc cggcagatcc gttttacgta atgcccattc ttcctccagc
      781 ggtttatcaa tggcggggag ggaatccagc aataatcggg tatatggatg tgccggaaca
      841 gtcaacacct gttgcgcttc ccctagttcc acaatctgcc cgagatacat caccgccacc
      901 cgatcgctca tatgacgtat caccgagaca ttgtgtgaaa tcagcacata agtcagcccg
      961 tgattttcct gtagcgttac cagtaaattg aggatctgcg cctgcacgga gatatccagc
     1021 gccgaggttg gctcatcaag cacaatcacg tcaggctgcg aagagagtgc tctggcaatg
     1081 gcgatgcgtt gccgctgccc gccggagaac gcatgaggca ggcggtcgag atactccgga
     1141 cgaataccca cctgcacagc cagctcctct gccaacgctc gccgctgttg ttcactacta
     1201 cgcttagcta tccagagcgg ttctgtgatg atgcgccaca ccggtaagcg cggattgagc
     1261 gaagagagcg ggtcctgaaa caccatctgc ataatgcgtt gtgagccaga acggatgtac
     1321 tgcccgtggc tcggttgcag catgcccatt aaaagctgtg cgagggtgct tttgccgcag
     1381 cctgactcgc cgacgatccc taaggtttca ccacggcgga tctgtaaatc aataccatta
     1441 atggcatgaa catgttccgt agttttacca agccagtttt tacgggccgg gaaattgata
     1501 tggacgtcgc gtaacgttaa taacgtgtca gacacttatg acctcctgct gcggatacca
     1561 acaggcgcag cgctggttgt tgtcaccaca cgctgtcagc gccgggacgt tttcacactg
     1621 tgcacctgcc gcatagcaac gatcgcgaaa agcgcagcct tcaggcaaat gggtgaggtt
     1681 tggtaccgtc cctggaatgg cgggtaatag ctggcgtggt actccatgtt ctggtgcgca
     1741 ttggagcaaa ccaatggtat acggatgccg gggatgatgg ataacatctg ccgtcacgcc
     1801 gctttcaatt acgcttccgg catacatcac gtaaacgcta tcgcacagtt gcgacaccac
     1861 ggccatatca tggctgatga acagaaccgc agttccactg gcgcgggctt tatgtttaag
     1921 caaacgcagt acctgcaact gcaccgtgac gtccagcgcc gtagtcggtt cgtcggcaat
     1981 aatcaattgc ggctcgcagg agaatgccag cgcaatcatt acccgctggc gcataccacc
     2041 tgaaagctca aacggatagc gcgacataac ttccacggca tccgggattt gcatctcttc
     2101 cagcagggca atcgctttag ctctggcttc ccgacgactt attggttgat gatggcggat
     2161 cacgtccatc atctgaagac ctattcgaag tgtcggattg agggcggtca tcggttcctg
     2221 aaagatcatc gccactcgtg cgccgcgcca ctgacgaagc tgtttttccc gagcgttaag
     2281 aacatcgtct cccagcagtg aaatctgccc ccgatgtacg caataactgc ccgtctgtag
     2341 cagacgcata atcagcattg cggtgactga tttacctgag ccggattctc ccaccagacc
     2401 gacaatttcg ccgcggttaa tctgcaagga cacattgttg agcgcgtgaa catcaccgtt
     2461 aaaaccgggg aaactcaaat gcagctgttg aatgtccaga acgggttggg tcatgactgc
     2521 tttcctcctg ctttcgggtc aagcagatcg cgaataccat caccaaagag attaaaccca
     2581 acggcggtaa gcaaaatcgc tgcccccgga aatgcgcaat accaccactg atcgagtaca
     2641 tagttgcgac cattcgctac catcgcgccc cattcagcac tcggttgttg agcacccagg
     2701 ccaataaatc ccaacgtggc ggccattaaa atcgcgctac cgatatccag cgatgcctgc
     2761 acgatcagcg gcggcaggga gttacgtaaa atatgccagc tgatcagatg ccaacgcgac
     2821 gcaccaaagg ttttcgccgc ctgaacatag gtatactggc gtacaactaa tgcttgcccc
     2881 cgcgccaggc gcacataaaa gggaattcgc acaatagcaa gcgccagcat ggcgttaaat
     2941 aaactcggcc cgagagcggc agccagtgcc attgtcagta ccaacgaagg aatcgacagc
     3001 ataatgtcca tgatgcgcat gatgatggcg tctgcgcgtc cgccaagcac accggataga
     3061 catccgagta gcgaaccaat catacccgca atggcaacca cgactaatcc ggcgagaatt
     3121 gattgctgac tgccgaccag tacgcggctg aacagatcgc gtcccacttc atcggtgcca
     3181 aaccagtgcg ccgcagaagg cggcaaaagg cgggcggtta aatcaatggc gttgggatca
     3241 tgcgtcgcca gccacggtga aaaaatcatc atcaatagca ttaatacaat gatgactgcg
     3301 ccggtcacgg tgagcggact gcctttcagc atccatacca gttttgcacc gttaaatcgc
     3361 gtttgttttt gtgggcgtac ggcggacgtt tcctcgctta gcatcattca ccacctccac
     3421 gtccgatacg cggatcaatc cacaaataga gcaaatccac caccaggttg accagcacat
     3481 aagcaaatga aaccacgacg gcaaagccca tcactgccgg gaagtcgagc gcctgtattg
     3541 atgttactac ccatgcaccc attccgggcc aggcaaaaac ggtttcggtg agcactgcgc
     3601 catacaacaa atcgcccagc gccaaaccca atacggtaat cgatgggatc aacgcattgg
     3661 gtagcgcata acataaaacg atataccagc cgggcaagcc gctggcccgg gcggtacgaa
     3721 tgtagtcttc actcaattgt tccagcatcg ctgagcggat ctggcgagcg acaattccca
     3781 ggtgaacgaa cgccagcgtt aatgccggta agatgagatg ttgcaacgca ttgaagaaga
     3841 cttcaccgtt gccttcaagc agcgcatcga gcagataaaa gccggtaacg tgcgttggtg
     3901 gatccagcca gtcatcaagc cttccgccgc cgggaagaat ttgcagatga ccataaaaca
     3961 gcacaatgac gcccagccca agccagaacg caggtgtgga gattccggta atggccatta
     4021 ttcgcaccag atgatccagc cagcgatttc gccagactgc agagagtatg cccagcggga
     4081 tgccaatcag gagtgccagc agcagggcac caaaagccag ctccagcgtc gccgggaaaa
     4141 atatacgcaa ctcttccagc accggacgcc cggtacgaat ggatgttccc aggtcaccat
     4201 gaaacaggtc gctgacgtag cggtaaaact gtacatacag tggctggtcc agtcccagtt
     4261 gctggcgaat attttccacg atagcatcgc tggcgcggtc acccgccagt aaccgcgccg
     4321 gatcgccagg gatcagatgc gagataataa acgtaatcac gcagacgccc gccaccacga
     4381 gcaccagccc ccagcagcgt tggcgtaaaa tactccagaa ggtcacgtta tttactcatg
     4441 gtattgatat tgaagacctg ttccagcatg ggattgaaca caaagccttt cacctctttg
     4501 ttcatcgcca gttggtagtt tttctggaac aggtacacat aagcagcgtc atcaatgacg
     4561 attttctgtg cctgctggta gtcccgggta cgctgcgtct ggtcggtggt cgcaagcgca
     4621 ttgcgcagta acttatcgac ctcactgttt tcatagaacg agcggttacc cggcagacct
     4681 tttttgtctg actcaaacca gtaattcata aacatatacg ggtcggcaaa atccggactc
     4741 cagttgccaa tcgcaatgtc gtaatcacct ttacccactc tgtcgcgcat ggtggcgttc
     4801 gccagctttt ccagcttcac attgataccc agcttgttga gactggattg tgtcgccaaa
     4861 gcaataggct cccagttcgg atcgttatcg gagtagagaa acgtcaggct ggtgggtttg
     4921 ctcgtcactt tatcccattc ggctttggct ttcgtttcgt catggttgta ttgcattgcc
     4981 gtcgcatcgt agccccacat gccttccgga atcgggccgc gcatctgttt tccgttacca
     5041 ctcagaatgc cgttaaccat gccctgataa tcggtagacc aggaaatggc ccgacgtaga
     5101 tccgcctgat taagaggcgc tttgctgtta ttcagataca gataggtaac gcgcagtgac
     5161 ggatactctg ccacattgac tttattttcc tgcttcaggg cgttgagttg atccaccggc
     5221 agcgcatcgg caatgtcaat gtcgccacgg gagagctgca ggcgacggga ggcactttca
     5281 ccgataattt ttaccgatac tcgcttaaag ttcggtttat tgccggggta atgcggattt
     5341 ggcaccagaa ctaattgctg acctttttgc cagcttttca gcataaatgg tccggaaccg
     5401 gcggtatttt gcgcgaggaa gccgcgagca tcatccgctg catgttcctt taagaccgcc
     5461 ggattgataa tggatgcacc gtcattcgcc agcgtgtaga ggaacggtgc gaatggttgg
     5521 ctaagggtaa acttcaccgt atgttcgtcg ggagcatcaa tctttaaatc tttgggaaat
     5581 gcttctgctg gcccctggcc gatttttagt agccgctcaa aagaaagttt tactgcttcg
     5641 gcagtgacag gtgtgccatc ggcaaattta gcattatctt tcagggtgaa cgtccactct
     5701 ttttgatcgt cagacgcttt ccagctactt gccagatcgc cttcaacgtc ggttgagcct
     5761 ttatcaccgt ccgttttgta ctgaaccagc cgctgataag acgggtaggt caccgtccag
     5821 tcgttattat ctattgttac cgccgggtcg agggtttgtg gatcggcggc cttaccaatc
     5881 accagcatat ctttcggtac ggcggcgtgc gaaaccggga aatttgtggc aaggacgagc
     5941 gcgagcaatg tgggacgaaa cgatatcgat ctcttcatga cagtttcctg attaactgac
     6001 gtgctgtgtg ccaggggata taaaacaaga gaattgatca gcgagcagag ggtaactcgc
     6061 tgcctggggt aattcgaagt gccaccattc gctggagata ccgacaaaac cgccaccagt
     6121 cattatcgca ttcagcaaca gccgattgcg ctgagcggcg ggcgggacgg aagggtgata
     6181 ggcatgggag cgctcgtgca tttcatcgaa ccctgcgccc atatcgagga tgttcccgtg
     6241 ctcatcacga agcgtcaggt cgatcgccgt gccacggctg tgattagaac cgaccgtcac
     6301 atcaacaaca tattgcgggt ctgggcaggc ttgccacaac atcgcctgtg cttgttgtgg
     6361 gcgatacgca tcgtaaatca ccagttgtaa gcctgacagc tgggcgatac tgatactttt
     6421 cgccagcgcg gtaatcgcat ccttgtgtag cagacaacgc gcttgctgat aaatagcttt
     6481 acctgtgatg ttatcagcgc aggcgtattt caattcgatc tccagatcag ggaagattac
     6541 ggctaaatca accagttcgg tggtatccga cataaacggt tcctgtataa gacaaaaatt
     6601 gctgcgcttt ccgcttatgc agatctcatg ccatgccggg ataagcgcca gaatgctggc
     6661 ttaaagttat attttttaac ttttgatcaa catttgtgca gcgtagtgca gttttggtgc
     6721 aagaggggaa gttaaggaag gaatctcccg gaatcgtagc tgaaatcaca gtatttaagt
     6781 gacagtgtca cgttaaatga aaacccgcga gtgcgggcga gaggaatttg tcagattttc
     6841 agcggtaaca cgctgctcat ccagcctgga atttcttcgg cgggtagggg gcgggaaaag
     6901 aaatatccct gaataacgcg acagtggatc ttgcgtagca tctcaaattg ctctttggtt
     6961 tcgacgcctt ccgccacgac ggttaaattg aggctttgcc caatgctggt aatggcttca
     7021 agtaaggcaa ggatgcgttt ttcggtcaga caacgatcga caaaactttt gtcaattttg
     7081 atttccgtta ccggaagact gactaagcgg gataatccgg aaaagcccgt accaaaatca
     7141 tctaccgata agcccacgcc catatcacgc aggatctgaa tgcgcttaaa gatttcggta
     7201 tcgtgttcca tcatcatgct ttccgtgatt tctaccgtca gctggtggcc gtcaataccc
     7261 caggcttgca ttgcatcaga cacctgatta ggcagctgat tactgcgaaa gtgcagcgcc
     7321 gacaagttca cggataacgc cgggatatga atattctggc tacgccattc tgctaactga
     7381 cggcaagctt ccgcgatgac ccagcgccca atattttcga tttcaccaat ctcttctgcg
     7441 agaggaataa accgtgaagg gggtacatga ccatgctggg gatcgtgcca gcgagcaagg
     7501 gcttcgatgc cgtacagttc acccgtttct gcgaagattt gcggctggta aaccagtttc
     7561 agttggttat tgctaatcgc ttctttcagc gctgcgccta aaaccaaacg ctcttttacc
     7621 atttcgttca tcgccgggct gaagaactgc cagccgttac cgccattctt gcgaatataa
     7681 tccattgcat tgtgagcagt ggagagtagg taatcgcggt ttttacccac gtcgtagctg
     7741 atgccaatac tcaaggtaag cgggaatggt ttatcgtcaa tcattatcgg cttgctgacc
     7801 acattccgta gctcatcggc gatctgggta atgttactga cgtcgttttc caggctcacg
     7861 aggacaaact gcgtaccttc gatacgacag agatactgat ccggtttgag tttttcacga
     7921 aagcgattga ccacttccag caatgcctga tcggcccacg catagccaag gctatcaatc
     7981 acatcctgaa tatggtcaac accgatgaga tacaccaccg gagagacggc tttgtcgacc
     8041 aggtcatcga ggtaattgtg caggttattg cgatttggca gaccggtcat cggatcaaat
     8101 tggatgagtt gttcaatatg ctggcggctt ttttcctgtt ccagcgccag cgcggccata
     8161 tgctgactga tatctgccac gcgttcgata aaggcgctgg tttctgctcc tgacgaggtt
     8221 ttaatttgca ggatccccgc aggcgcgcca tcacgctgac gaatggtcgc tgaccagctt
     8281 tgcgcatttt gaacttctgc accgtgcgaa gatgacgccc agtgtatcgg catcccgttg
     8341 cgcagtgcga acagcgaaac atgcgattcg ttgagaacag attcgatgtt acgacaaatg
     8401 atttccccca tttcatgaaa tggcgggctg ctgcacatgg cggcgagaat attgccttca
     8461 agctggcgga tttgccgttc ttcggtgata tccgagaaag tcattaccag gttctgcaga
     8521 tgcgcgagca cgtcataaac cgggctgata gaggctttaa tccagatttt ttcaccggtg
     8581 cgcgtcaaca gcagaaattc gtcctgatcg cgggcggttt tccatagcaa ctgttgtaaa
     8641 cgaatgcggt tatcggcagg gaattcagga atgttcagga gtgtatcggg ctgcataccg
     8701 ctggcttcgc taatgcagta accaaacatt tcggtaaatg cgcgattgca ctgcacaata
     8761 tggcgttccg gatcgaggac aatcaccggt cggtcgagat ggtcaacggc aataatcaat
     8821 tgtcgagtct gttctttttg cgccatttct acgctggcat cccgtaccag cgccaggtaa
     8881 taaactttcc cctcggcgct cactttcgat agcgcaaaac gggtccagat tttactgccg
     8941 tcttttttct ccagctgcag ctcccgactc atcccctcga cacgcgcttt accgccttca
     9001 cggttatgac gaatgtattc aggatgcgca ggacgcaaat cccgcggaat cagcatatca
     9061 atgttattgc caatgacttc ttcacgtttg tacccccaga gcttctctgc ggcggggttg
     9121 aaaaacatca cttcatcatt ttcgttaatt aacaccgcac ccatcatatt ttgctcaagg
     9181 gcggggaaaa aaatgccatc ggcggcatta tccgcatcgg ttagcttcat gattacctct
     9241 gcatcctggc gcatctaaag actggctttc cagagttcaa cacggtttct acctcgtctt
     9301 ttggcgatat acagagcttc atcggctatt tgaatgaggc gctcatagtc aggatgacca
     9361 ttaaacatgg cggcaccgat ggaaagtgag agggcaatat cctcgccgtt tgcggctttc
     9421 agtttggttt tctccacccg actgcgaata cgttctgcgg tacgtaacgt ttcgttttca
     9481 gaagcttcag tcaaaacaat gataaattca tcgcccccgt agcgaaaaac ataatcacta
     9541 ctgcggacgt tgtcataaaa ggcctgagag actttacgca gaatttcatc gccagtgtta
     9601 tggccccacg tatcgttgat ctctttgaat ttatcaacgt caataatcag cactgacagt
     9661 ggtgtaccgg tccggttggc atgggcaatt tcgcgtttga agatagtcgg caggaaacgg
     9721 cggttaagta atttcgtcag tacatccata ccgacttcgt ggcgcgatac ttcttcaaac
     9781 aattcacgca gcaaggtaat aatttgcgat acagtatttc ttatctgtaa taaaaatttc
     9841 acccgcagac ttctgttatt caaattcctg gtgttacgca tggtttgatt gaatataccg
     9901 t
//
LOCUS       DAZKGS010000158         9877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKGS010000158 DAZKGS010000000
VERSION     DAZKGS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..616)
                     /locus_tag="ACHOQY_003940"
     CDS             complement(11..616)
                     /locus_tag="ACHOQY_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFP9679945.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLIINMLMCAGHY"
     gene            complement(613..2301)
                     /gene="yfaA"
                     /locus_tag="ACHOQY_003941"
     CDS             complement(613..2301)
                     /gene="yfaA"
                     /locus_tag="ACHOQY_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9679946.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2450..5077)
                     /gene="gyrA"
                     /locus_tag="ACHOQY_003942"
     CDS             complement(2450..5077)
                     /gene="gyrA"
                     /locus_tag="ACHOQY_003942"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9679947.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5224..5946
                     /gene="ubiG"
                     /locus_tag="ACHOQY_003943"
     CDS             5224..5946
                     /gene="ubiG"
                     /locus_tag="ACHOQY_003943"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9679948.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6074..9808)
                     /gene="yfaL"
                     /locus_tag="ACHOQY_003944"
     CDS             complement(6074..9808)
                     /gene="yfaL"
                     /locus_tag="ACHOQY_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP9679949.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEDYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2307 a         2736 c         2379 g         2455 t
ORIGIN      
        1 tttattttga ttaataatga ccagcgcaca ttaacatatt gataattaat gaaattttaa
       61 tatacaactt ccatgtcata agttgttgca gactgactgc gcgcattccg ttgtcagttt
      121 tcgtggcgct tccggtgtgg tagatgacgt aacgccccat ccagaccatt aagtgctggg
      181 catcgccctg atcgaaaaaa atcatatcgc caggcagcgc ctggtttatg tcctggccaa
      241 taaactggct gttgtactga atcaaattaa tcgcggtcac gtaggggccg gttttcccgt
      301 tcccctgatt ccagttttgc gccagttgac gctgtccagg tgttagcgtc atctctggcg
      361 gcaaatactg gctagagaaa ccgttacttt ttagccattt actatcgtga actttcagcg
      421 tctcgttcgc cgcaaatcgc accaggcccg cacaatcctg ctgataccag cgtggacttg
      481 gcccctggcg gagctgttct tgtgcaatgc gtacaaacca ggcgcgaaac agcccggatt
      541 gttcgacatt cagcatttcg ctatgggcaa caacacaaca cagccagcaa atcagcgcca
      601 gcagcccgtg cctcatagcg gctgccaggt tatgggtagc cactgccagg cggcaccggg
      661 ttccatctgg gccagcttca tgacataacg cggttgttga gataaagcgt ccagcttcgg
      721 cattaataaa gtttgtgcgg cgttataaaa aaccggttcg agattcttcg gcagactggt
      781 caacgtttcg ttacgcagca gtttcgccac gccttgtgga ttgatataga gcggaacgat
      841 gccatcagtg ggtattacat ccaccattgc cgggcggttt ttattcagtg tttgcagggc
      901 gttattaacc agcgtgtcat cgagggagaa aagcagcgtt ttgttttgca tcgccagcga
      961 cacgcggaaa aaataatccg acattaattg atcgggttgc gccgcctgcg ctttcggata
     1021 ctgaccgtat cgggaactca cttcgcgacg ccagatttgc gcttcgccct gctgagtctg
     1081 gcttatcggc aaaacacctt ctggcgcttt gctttcgtgc gcaccaatat tttgcgtaaa
     1141 cagtttccct ggcaattgcg cctgttcggc agtgccatca aactgaccga caaacagcgg
     1201 ggtttgcaat tttgagtctg cataccagca cagccccgcg gcaccgtcta acgccccatt
     1261 caacttgtcg ttttcctgac tgatgtgcga aagcatctct tcggcaatac cgtgtgaata
     1321 cggcaccacc acacagaagc tggccccggc aggcatactg ttccagaccg gcgtaaaatc
     1381 gaaactggta tctacgctgg cggattcatc atttaacgcc acaaaactgt gccagccgtc
     1441 gttacccatt tcgaagcgta cgccagcaaa agaaggcatt aaccgctggt agccaaaacc
     1501 cagcaacctg gcgctgacca cgatgcgctg gcgtactggc gttttttcag cagtacgctc
     1561 ttccaggcca aagcttgctt gccagcgttt tttgccgctc aacaaatcac ctgcgatcgc
     1621 cgtggcttcg gtatcctgct gatcatcttt aaacaacata tccgtgctgg aaaacaccag
     1681 catcttgtcc tgataagtcg cgaacatcag ggcgttattg ccgttatagc gcaactgata
     1741 aacaggaaca gtttcactat ttatcttgat gctactgatt tccgttttgc ttaactggct
     1801 gtcgctggta gcggcaaaca acaatggctc cagcagtttg cttaaaccac tgcgctggat
     1861 cagcaccata taatgtgaaa gatggccttg tttatcgtgc cacagcgctg cctgcgcggg
     1921 ctgatctaag agtgacgaaa agagcttatc tttcagcgtg agatcgtgtt catagacaat
     1981 acgacgaatg ctgccttcaa tgcccagacg atcggcatga ttctgataat agaaaacgaa
     2041 atcttcgctc agaacatcgt ggagaaacgg aatggtgagg agatctttgg gaagctggct
     2101 cagagagtcg ctgtcgagaa agaggtccgg ctcattgaga tcgatttgca gattgttgtg
     2161 caccaccagc ggcgacaacg ttttttctgg cccactgcca gcatattgca acgcccagac
     2221 gccagcggaa agcagtgcta ttgcgccaaa acctacaaga ccatagaacc gccagccttt
     2281 cgcctttttt tcaccactca ttgccacatt ccttgtgtat agccagccat tttttacggg
     2341 cacagccaaa ctttaccgtg ccctaatacg acaaaagccc agactttgca gcctggactt
     2401 ttcaattcaa acaagggaga tagctccctt ttggcatgaa gaagtaaaat tattcttctt
     2461 ctggctcgtc gtcaacgtcc acttccggag cgatttcatc gtccccttcc gcggcactgc
     2521 catcgatggt atccagatct tcctcgtcaa ccggttcagc aacacgttgc agacccacta
     2581 cgttttcatc ttccgcagta cggatgagga tcacgccctg ggtgttacgg cctacgatgc
     2641 tgatttccga aacgcgagta cgtaccagcg taccggcatc ggtgatcatc atgatctggt
     2701 cgcagtcatc tacctgtacc gcgccaacca ctaaaccatt acgttcagta accttaatgg
     2761 agataacccc tttcgtcgca cgcgacttgg ttgggtattc cgccactgcg gtacgtttac
     2821 cgtaaccgtt ttgcgttgcg gtgaggattg cgccatcgcc acgaggcacg atcagagaga
     2881 cgactttatc gccttcacct aagcgaatac cgcgaacacc ggtggtgttg cagcccatcg
     2941 cacggacaga agactcttta aagcgcacca ctttaccttc agcggagaac agcattactt
     3001 cgtcttcgcc gctggtcagg tcaacgccga tcagctcatc gccgtcaacc agtttgatcg
     3061 ccactttacc ggcggtacgc agacggttga actcggtgag gacagttttc ttcacggtac
     3121 cgttagcggt cgccatgaag actttcacgc cttcttcaaa ctcggtcact ggcaggatcg
     3181 cagtgatacg ttcgtcctgc tccagcggca gcaggttgac gatcggacga ccgcgcgcgc
     3241 cacgagtggc ttccggcaac tgataaactt tcatcgaata gacgcgacca cggctggaga
     3301 agcacagaat atggtcgtga gtgttcgcca ccagcagtcg gtcgataaag tcttcttctt
     3361 taatacgtgc ggcagattta cctttcccgc cacgacgctg cgcttcgtat tcagaaagcg
     3421 gctgatactt aacgtagccc tggtgagaga gcgtcactac cacatcttcc tgagtgatca
     3481 gatcttccag gttgatgtct gcgctgttgg cggtgatttc agtacgacgt ttgtcaccga
     3541 actgttcacg aaccagctcc agctcttcac ggatcacttc catcaaacga tcggcgctac
     3601 caagaatacg caacagttcc gcgatctgat ccagcagctc tttgtattcg tcgagcagtt
     3661 tttcgtgctc aaggccggtc agtttctgca aacgcagatc cagaatcgcc tgagcttgct
     3721 gttcggtcag gtagtacaga ccatcacgca cgccgaactc tggctccagc cattccggac
     3781 gcgcagcatc gtcgccagca cgttcgagca tcgcggcaac gttgcccagc tgccacggat
     3841 tagcaaccag cgcagttttc gcttctgcag gcgtcggcgc atgacggatc agttcgatga
     3901 tcgggtcgat gttcgccagc gccacggcta atgcttcaag gatatgagca cgatcgcgag
     3961 ctttacgcag ttcgaaaata gtacgacggg tcaccacttc acggcggtga cgaacaaacg
     4021 ccgcgatgat gtctttcagg ttcatgatct tcggctgacc atggtgcaat gccaccatgt
     4081 tgataccgaa agaaacctgc aactgggtct gggagtagag gttgttgagc acaacttcac
     4141 cgaccgcatc gcgtttcact tcaatcacga tgcgcatacc gtctttgtca gactcgtcac
     4201 gcagcgcgct gatgccttcc acgcgttttt cttttaccag ttccgcaatc ttctcgatca
     4261 ggcgcgcttt gtttacctga tacggaattt cgtggacgat aatggtttca cgaccggttt
     4321 tggcgtcaac ttccacttct gcgcgagcgc ggatatacac cttgccgcga ccggtacggt
     4381 aagcttcttc aataccgcga cgaccgttaa tgattgccgc cgtcgggaag tccggccccg
     4441 ggatgtgttc catcagccct tcaatgctga tgtcttcatc atcgatatac gccagacaac
     4501 cgttgatgac ttccgtcagg ttgtgcggcg ggatgttggt tgccatacct acggcgatac
     4561 cggaagaacc gttcaccagc aggttaggaa ttttggttgg catgacgtcg ggaatttttt
     4621 ccgtgccgtc atagttatca acgaaatcga ccgtctcttt ttcgagatcg gccatcagtt
     4681 catgggcaat tttcgccaga cggatttccg tataacgcat tgccgccgca gagtcgccgt
     4741 cgatggaacc gaagttaccc tgaccgtcta ccagcatgta acgcagcgag aatggctgcg
     4801 ccatacggac gatcgtgtca taaaccgccg agtcaccatg gggatggtat ttaccgatta
     4861 cgtcaccaac gacacgggca gattttttat aggctttgtt ccagtcattg cctagtacgt
     4921 tcatggcgta aagtacgcga cggtgtaccg gcttcaggcc atctcggaca tctggcagcg
     4981 cacggccaac aatgaccgac atcgcataat ccagatagga gctcttcagc tcttcctcaa
     5041 tgttgaccgg tgtaatttct ctcgcaaggt cgctcatcta accgctatcc ctctactgta
     5101 tcccggattc aaaggtcgca aattataaca cagccgcgca gtttgaggta aacctatacg
     5161 ctttattcac atccaatgcc tgatatactc gtttgtcttg ccaattacgg agtagaagtg
     5221 ccaatgaatg ccgaaaaatc gccggaaaac cataacgtag accacgaaga gatcgctaaa
     5281 tttgaagccg tcgcctcccg ctggtgggat cttgaaggtg agttcaaacc gttgcaccgc
     5341 attaacccgc tgcgtctggg ctatattgcc gagcgtgctg gcggtttatt tggcaaaaag
     5401 gtgctcgatg tcggttgtgg cggcggcatt ctggccgaga gtatggcgcg cgaaggcgcg
     5461 acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaaact gcacgcgctg
     5521 gaaagcggca ttcaggtgga ttatgtgcag gaaaccgtgg aagagcacgc ggcaaaacat
     5581 gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtccc cgatccgcag
     5641 tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt tttctcgacg
     5701 cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata tattttgcgc
     5761 atggtgccca aaggcacgca tgatgtgaag aagtttatta aaccggcaga actgcttggc
     5821 tgggtagatc agacaagcct taaagagcga catataaccg ggctacatta caacccgctc
     5881 actaatactt ttaaactcgg ccccggcgtg gatgtgaact atatgctgca cacgcagaat
     5941 aagtgaggtt gttgtttggc cgagccaatg cctgatgcga cgcttgccgc gtcttatcag
     6001 gcctacaaat gctccccgta ggccggataa ggcgtttacg ccgcatccgg caaccgtgcc
     6061 gactagacag tgattaccat ttcaccgtca tcgacaaaaa ccctgccgtc tgggcaaaat
     6121 catcactccc tttctgccac gccacgctgc cgcgcaggga cactcgctga ctgatattgc
     6181 ccgtgactcc cacttttatt tcaccccgtc gcttcaccgc atcgtcactg atagtgctgc
     6241 catcttcttc aatttccgtc gcatggggat cgtgataata attcagatct aatgttggtg
     6301 tgacaccaac cgcggtacgc cattcgctgt gtaaacccag ccgcgtctga atatcatccc
     6361 cctgcgattg tgacacgcgc gcatggttag cggcggtaaa atcatcctgc tgcacgccct
     6421 gataaatcac ctgcgcctgc ggttcaatca ccacaccacg ccccggtaac cactgatacc
     6481 cggcttccag cgaggcgata atccccgacg agtggtaatg atctgtgcca tcgtcttgtt
     6541 cggaaacatc attgttaaac cacgcgtatt gcagccagct atccagccag gccccctgct
     6601 tctgattacc gtgctgatac cagcttgagg tcagcccaac ggcataaccg tggttctggt
     6661 tatcggcgcg agttccggtc atattcgagc ggctctcgcc ctgattatca ctgtagccac
     6721 caaccgcccc aagcatccac tcgccatcat cgccccagcg cccgctaaac aggtcaccgc
     6781 taagctgcac cgtagaagtg tcttcatgct gagccagttg ccctgccgcc gtgtaatgat
     6841 aacgtccgcc gataacacgt aaattcagcg tctgaccatt gccacctgca tgatcgcgtc
     6901 gctccatcat aaacgcctga tttgccgccc gcagattatt gagataaccg ccaactttgg
     6961 cattcagcac cggctggtaa gcggggattg gatcgggtgt tgggtcagga tcgggtgttg
     7021 ggtcagggtc tggatcaggt ggcaatggtg gcgttacttc ttgcgatcgc agataccagt
     7081 cgttgttatc ttccaccagc gtgtagtcat atgctcccat attgacgtag ccgctgcctg
     7141 ccagactgaa cttcgcattg ttttgaaact gcgtgggatc ggctgcgaaa tcaaccactt
     7201 taatgcctgt cgatgtcggc tcaccaatcc ctgtaatgga gttaaccacc acagttgtgt
     7261 tgccagcagt attaccgttc aacaccaatt gatcgcttac cgagtcatcg ccgtttaatt
     7321 cgctatcgag cagtagcgta ccgccgccgg tataatcacc gtttaccgtc agcgtatcgc
     7381 cagcgacgcc attttgcagg ctaaccgcac cggacgtatt ggtcagattg ccattaaccg
     7441 tcacgtcgga aagataggtc aggctgtcag tttcaaggtt ggtattaagc tcacctgtca
     7501 acgtcacgcc atcggtggca ttcaccagcg tcccatcgcc gttaaacagt gaggcattaa
     7561 gggcgacaga agtatcctgc ccggtcaggc gcaaaaccgt accatcgctg atgtcgatag
     7621 tgccgctgga agtagcatca atagattgaa tatcctgatc cgcgccagta acgaacgtcg
     7681 ccccatcacc gacccacagt gacgaagcat aaggaaggat atccgcgaca tcgcctttca
     7741 gggtgccttc ttcgacacgt accgccgact gcgttgtacc gctggcggtc agctccagcg
     7801 tacccgcccc cgttttagtc aatgtgctac cctcatcctg atgctgcccg ctgctgtcag
     7861 ccatcagtgc gccccactgc gtgtctaccc ccgcatcaac tgccacttca ccgtcggcgc
     7921 gcatttcaat atcacgccca tgctcagtcg tcgtagagcg agtatcgaca ttggcagtaa
     7981 tctccatcac gctttcttgt tgcttatcgg taaagataac ctggcggtta tagtgggtat
     8041 ctccaagctg tgagttgtcg ctcacctcca gcataccgga ggcgatttgt gttgtgccca
     8101 aataactatt attgtttgcc agacttacct gcccgtcgcc cgcatcgacc accacgtaga
     8161 cgccatcttt cccgtcaccg ccgatatgca tatcgtttcc acctgcaaga tctaccacat
     8221 cctgactgcc gataaccgtt ccgctgctgc cgctgacttc aaggccatcg ttgtatgatg
     8281 tgccactctg ccaggtggag aaatcagaga gatcgagcac accgccgttt aacacgatcg
     8341 actgcggatc gtcctggaga gcggtaagat ctgccgcgtc gccttccagc gaaagcaccg
     8401 caccatcatc aacgactata tcgccggtta gcgccatcga ctgcgccccc gacagcacgt
     8461 agctgccgtt ttgcgcaatg gtgagctgac cagcaccttc gatggtgcca gcaaaactgc
     8521 cttgattaac agtaacattg ccaccagcat cgatatttaa agtgccattc tgaaagcccg
     8581 tcaatgagtg cacaaaggtt tgttgggtgg agccaacgtt tagttctgcc tgattctggt
     8641 acttatcaat actccctatc gtcagaccgt agcagtcttg cggatcgtcc tggcaatgcg
     8701 tatcgccgac attcatcagg gagttgctgc gacccagggt aacttcaccg ttttcaatct
     8761 gcatctcacc agtaaagtca ttgttatctg cattaagtac cagatcgccg gaacctgttt
     8821 tggtgatcaa cccggtacca gcaatagagt caacagctcc gtcattctct gtattgccaa
     8881 taaccagcgt ttttccgtcg gcaatatcaa aggtaacttc acttaagccg agatacataa
     8941 agccacccgc cgcagtggaa ggaccatctc catagcctgc tgcgctgttg ttctcatcca
     9001 ctaacacgcc gccgttctgg ctgtagctgt catcaacaga aatatcaata agatagggag
     9061 ccgtcgcgct attggtatat atcgccccgc cataatcttc ggcagtgtta tttgtaaagg
     9121 cggtgttatt tattatcgta taacctgaag gatgcttgct gtcgctatta ttatcggtaa
     9181 catcgattgc cccgccatcg ccatcactgt aacttgttga tgtatatgcc tggttgttat
     9241 caaaaataac atcacttaaa taaacgtcat tattaatggt ataaattgcg ccacctttgc
     9301 catcattagc gatgttattg cgaaacatgg cgttagtgac acgtaaatcg acgacaccag
     9361 tatcattagt accagaagaa tagattgcgc caccatagcc gcctgcgacg ttaccagaaa
     9421 aaataacatc agtaagattc agcgttgagt tttctttagc aaatatggcc ccgccgttat
     9481 tatattctcc tgtaacggtg ttattggcaa acagagtcat tccagtttca ttttctggta
     9541 atagtgaaaa ttccgctcct tgttgcaaaa atacggctcc tccgctggcg ttattggtca
     9601 tatccgaaaa aaccagccat tgcccatcgg cgacactcca gtcctgcgta atgcctgaaa
     9661 ggctttgcct gctggcgtga caactcgctt tgatatcata tccctggcat aaatcaatgg
     9721 ccgccgcgaa accgttagca gagaaaagag atgcaatcat tgacgggagt agagataaat
     9781 actccttgcg tagaaagata atccgcatat tcatctaacc atcattttat ataagacggc
     9841 atatttaaac gcattataca atatgggtta tctttct
//
LOCUS       DAZKGS010000159         9850 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKGS010000159 DAZKGS010000000
VERSION     DAZKGS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..343
                     /gene="bdm"
                     /locus_tag="ACHOQY_003945"
     CDS             128..343
                     /gene="bdm"
                     /locus_tag="ACHOQY_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9679950.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            445..582
                     /gene="sra"
                     /locus_tag="ACHOQY_003946"
     CDS             445..582
                     /gene="sra"
                     /locus_tag="ACHOQY_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9679951.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            739..2436
                     /gene="maeA"
                     /locus_tag="ACHOQY_003947"
     CDS             739..2436
                     /gene="maeA"
                     /locus_tag="ACHOQY_003947"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9679952.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2570..3580
                     /gene="adhP"
                     /locus_tag="ACHOQY_003948"
     CDS             2570..3580
                     /gene="adhP"
                     /locus_tag="ACHOQY_003948"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9679953.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3727..4011
                     /locus_tag="ACHOQY_003949"
     CDS             3727..4011
                     /locus_tag="ACHOQY_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9679954.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4062..4715)
                     /gene="fdnI"
                     /locus_tag="ACHOQY_003950"
     CDS             complement(4062..4715)
                     /gene="fdnI"
                     /locus_tag="ACHOQY_003950"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9679955.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4708..5592)
                     /gene="fdnH"
                     /locus_tag="ACHOQY_003951"
     CDS             complement(4708..5592)
                     /gene="fdnH"
                     /locus_tag="ACHOQY_003951"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9679956.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5605..8652)
                     /gene="fdnG"
                     /locus_tag="ACHOQY_003952"
     CDS             complement(5605..8652)
                     /gene="fdnG"
                     /locus_tag="ACHOQY_003952"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8065..8067),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9679957.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8884..9765
                     /gene="yddG"
                     /locus_tag="ACHOQY_003953"
     CDS             8884..9765
                     /gene="yddG"
                     /locus_tag="ACHOQY_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9679958.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2393 a         2582 c         2488 g         2387 t
ORIGIN      
        1 aaatataaaa atcatcaacc aggactaatc ttaacaacga agcggcaaat atttgccatg
       61 ctgaatgtgc tttataaatc tgcgatccgt agcagacacc ataaatacac agacacggag
      121 aatcactatg tttacttatt atcaggcaga aaattcaaca gcagaacccg ctctggttaa
      181 tgccattgag caaggccttc gggcagagca cggtgttgtc actgaagatg acattctcat
      241 ggagctgacc aaatgggtgg aagcttctga taacgacatc ctcagtgata tctaccagca
      301 aacgatcaat tatgtggtca gtggccagca ccctacgctt taaggtgcta tgcttgatcg
      361 gcaacctaat ttaggggttt agcacgtgtt tcttcgctac ggcgatgttg tccttaaaac
      421 tagcaacagg attgaggagt taaaatgaaa tcgaaccgtc aggcacgtca tattcttgga
      481 ctggaccata aaatttctaa ccagcgcaaa atagttaccg aaggtgacaa atccagcgtg
      541 gtaaataatc caaccggcag aaaacgcccc gctgaaaagt aattcataac catcagtcct
      601 caatgacgat taaacaccat tgcctgcgca atggtgtttt tgtttttatc tgctctatac
      661 ttggggccga cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt
      721 caaaaaagag tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct
      781 tacgctggcc ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg
      841 gaagaacgcc gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa
      901 gaacaagcgg aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac
      961 atctacctgc gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat
     1021 catcttgatg agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt
     1081 ttttctgaga tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccggcacaat
     1141 atggacgata ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac
     1201 ggtgaacgta ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt
     1261 aaactgtcgc tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg
     1321 ctggatgtcg gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt
     1381 aatccacgta tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg
     1441 aaacaacgct ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg
     1501 ccgttactta accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact
     1561 gcggcagtaa cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc
     1621 gagaaaaaaa tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc
     1681 atcgcccaga cccagcgcga aggattaagc gaggaagcag cgcggcagaa agtctttatg
     1741 gtcgatcgct ttggcctgct gacggacaag atgccgaacc tgctaccttt ccagaccaaa
     1801 ctggtgcaga agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg
     1861 ctggatgtgg tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg
     1921 ctgtttacgg aagagatcat tcgtgagatg cataaacact gtccgcgtcc gatcgtgatg
     1981 ccgctgtcta acccgacgtc acgcgtggaa gccacaccgc aggacattat cgcctggacc
     2041 gaaggtaacg cgctggtcgc cactggcagc ccgtttaatc cagtggtatg gaaagataaa
     2101 atctacccta tcgcccagtg taacaacgcc tttattttcc caggcatcgg gctgggtgtt
     2161 attgcttccg gcgcgtcacg tatcaccgat gagatgctga tgtcagcaag tgaaacgctt
     2221 gctcagtatt cgccgctggt gctgaacggc gaaggtctgg tactaccgga actgaaagat
     2281 attcagaaag tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc
     2341 gtggcggtga aaacgtctgc cgaagctctg caacaagcca ttgacgataa tttctggcaa
     2401 gccgaatacc gcgactaccg ccgtacctcc atctaagcct gcgcccggta gtgaaggcta
     2461 ccgggctatt tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat
     2521 ctcatttgcc tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaaggctgc
     2581 agttgttacg aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca
     2641 tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa
     2701 gaatggcgat tttggtgaca aaactggggt aattctgggc catgaaggca tcggtgtggt
     2761 ggcagaagtg ggtccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg
     2821 gttctacgaa ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg
     2881 ttcagttaaa aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt
     2941 cgccgattac gcggtaaaag tgccagatgg tctggactcg gcggcggcca gcagcattac
     3001 ctgtgcgggg gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg
     3061 gattgctatc tacggtcttg gcggtctggg taacctcgcc ctgcaatacg cgaagaatgt
     3121 ctttaacgcc aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga
     3181 aatgggtgca gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga
     3241 gaaaaccggt ggcgctcacg ctgcggtggt aacagcagtt gctaaagctg cgtttaactc
     3301 ggcagttgat gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc
     3361 tatgagcctg gatatcccac gccttgtgct ggatggcatt gaagtggtcg gttcgctggt
     3421 cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc
     3481 gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg
     3541 caaaatccgt ggccgtatgg tgattgattt ccgccgttaa gaggcctttg ctgcgagtgc
     3601 cagtcctggt gtcgcagcat cgcactctct ccgacattaa atggctaaat ggattattgt
     3661 tccttcctac gcaacaataa tgatagtgtt cctgatgaag gaacactact gagagtaaag
     3721 acactgatga aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac
     3781 gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg aaattgctcc ctcaacggca
     3841 agccgattgc tgaccggaaa agcagctttg acaccagaaa tggcgattaa actttccgtg
     3901 gtgatcggca gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag
     3961 gcagaaaaaa cggtggatgt gtcgcgacta cgccgtctgg ttacgcaata aaaaaatggc
     4021 gcttcttaaa gcgccatttt cagcttaaag ttcaaaagag attatatccc ttcttcactc
     4081 tcttttttcg cttctgcctt ctcgatttca cgataccagc gcggatggtg tttcttcgcc
     4141 cagcgacggc tcaccttccc ttcgatcatc cctttaatcg accctttcac ccagaatgcc
     4201 atatacatat ggatcaggat ggcgtggatc aggatgatac ccgcagccgc gtggatcagc
     4261 aggctgtagc gaacaacctg catcgggaag tactgcgcaa agtacggacg ccagataatc
     4321 accccggtca ccagcagcac gaaaatcatg ctcatgatcg accagaacat catcttttgc
     4381 ccggcgttgt acttaccgac atccgccact ttatgctcat tgcctttcaa tacttcgaca
     4441 atgttcaaca gccacggaat atctttctta tccgggatgt tgtgatgcac aaaacgcaca
     4501 aacataaaca tcagtgcgac gaaaatcgca atgccgaaaa acgggtgcaa aatgcgtccc
     4561 atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc
     4621 ccggacagcg ccaccaggaa gaagcaaatc accacggtcc agtgacaggc gcgatcaata
     4681 aatttggtgc gcacaatcat tttcgactta ctcatgatga tcctcctcgt catcgtccac
     4741 ttccttattc gggccaatac cgatgtagtg gaaaatcaac ccggcaaaag tggcaataaa
     4801 gccagccgct gccagcggtt tcaacgcgcc tttccacagg cttaccgagg tgtcgatctt
     4861 cggatctttc ggcagaccgt gatacagctc cggctgatcg gcgtgatgca gcacgtacat
     4921 aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca gcatgttcgt aaccacgcgc
     4981 tttcagtttc gccacgcgct gttccgccag ctccagcatc tccttcttgg tgccgaagtg
     5041 gatagccccg gtcggacagg ttttcacaca agccggttcc tggccgacgc tgacgcgatc
     5101 gacgcagagc gtgcatttat atacccggtt atcctctttg ttgaggcgcg gaatattaaa
     5161 cggacacccg gcaatgcagt aaccacagcc gatgcagttt tccgactgga aatcgacaat
     5221 cccgttagcg tactgaatga ttgcaccagc agacgggcac gccttcaggc agccgggatc
     5281 ttcacagtgc atacagccgt ctttacggat cagccactcc agcttgccgt tctgttcggt
     5341 ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga tcggcggggt tatcgtaaac
     5401 cccgacgcag tgccccactt catcacggat gtcgttccac tccgaacacg ccacctgaca
     5461 ggctttacag ccgatacagg tggaaacgtc gataagtttt gcgacttctg ctttgtaatc
     5521 acgcacctga gaaggcggcg tgatggagtt agttgcggac cttttgataa tgtcctgcgt
     5581 ttccatagcc atctgttcgc ccccttacgc cttctcgatg ttgactaaga acgctttata
     5641 ttccggcgtt tgcgagtttg catcaccgac attcggcgtc agagtgttag cgatataacc
     5701 tttacgcgcg acaccctcaa agccccagtg gattggaata cccaccgttt caacctgctg
     5761 accatttaca ttcagtggtt tcagacgacg cgttaccaca gccaccgcgc ggataaagcc
     5821 acgcttgctt gagacagtga cacgatcgcc attattaatg cctttcgccg ccgccagcgt
     5881 ttcgctgatt tccacaaact gttccggctg agcaattgcg ttgagcaatg cgtgcttggt
     5941 ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc acatacggga actgctcttt
     6001 tttacccatc cgcagtgcgt cttgttcata cagacgaaca accgggttag acaccacgtt
     6061 cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc tcgtagtgtt ccgggaacgg
     6121 accttccgcc attttgttga tggcaaacag gcgtcccatc ccttccggct gcatgataaa
     6181 cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca ggaatatcgt tacccgtcca
     6241 cttgctgccg ttccactgga tcagcatccg tttcggatcc cacggtttac cgttgatatc
     6301 cgccgaagca cggttgtaca gcacgcgacg gttgagcggc cacgcccagg cccatcccag
     6361 cgtattcccc agaccggacg ggtcggagtt atcgcggtta gccatctggt tgccctgctc
     6421 tgtccagcta ccggtgtaga tccagcaaga agatgcggtt gtaccgtcat cacgcagatg
     6481 cgcaaagcta ctcagcaact gacctttctt cgcaatcagc acgccattag catcatagag
     6541 atcttccagc gcatagccgt tgttctcttt agctacttcg tcagattgcg gttcgtgcgg
     6601 ctgcttgtag ttccagctca tcttcatcag cggttctacg cctttaccac cttcggcctg
     6661 gtacagctcg cgcaggtgat ggtagatacc cgccagaatt tcaccgtcgt tacgcgcttc
     6721 gcccggcgca tcctgacctt tccagtgcca ctgcagccag cgaccggagt tagcaataga
     6781 accatcttct tcagcaaagc aggtcgaagg cagacggaat acttcagtct gaatagacgc
     6841 cggatcgaca tcgttcgact cgccgtggtt ctgccagaag gttgaggttt cagtcaccag
     6901 cggatcgata accaccatgt acttcagctt gcttaggcag ctcaccactt tgtttttgtc
     6961 cgggaaggac gcaaccgggt taaagccctg gcagaaataa ccggtgactt tgccttcatc
     7021 catcatgttg aaatacttga tgacgtcgta ggtctggtcc cacttcggca gccagtcata
     7081 gccccagttg ttctctttct gcgcggcatc gccatagaaa gatttcatca ggctaacgaa
     7141 gaacttcgga tagttgctcc agtagttcac ctgaccagcc agcgtcgctt tcggcgtgtt
     7201 cgcttccaga tacgactgca aatcaacctg tttttctgac ggcagcgtca gataacctgg
     7261 caggctggta gagagcagac ctaagtcagt cagaccctga atgttggagt gaccacgcaa
     7321 tgcattcacg ccgccaccgg ccatacccat gttaccgagc agcaactgga tcatcgccat
     7381 agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt
     7441 ggttgtgcga tccggtgcgc tggtggaggc cagcacttca cacactttca ggaagtcggc
     7501 ttttggcgta ccacagatgt tttcaacaac gtccggcgtg tagcgggaaa cgtgcgcttt
     7561 cagcaggttc cacacacagc gcggatgagt cagtgtttca tcgcgtttcg catagccgtt
     7621 ttcatcgaac tgatagttcc aggacgattt atcgtattgc cgtttttcag cgtcgtagcc
     7681 gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc agcaggctgg cgttggtgta
     7741 atgcttaacg tattcggcgt tgattttgtc gttttcgatc aggtagcgca aaacgccaga
     7801 caggaacgta atgtccgtac cggaacgaat aggtgcgtaa atatccgcca cagaagcggt
     7861 acgcgtaaaa cgaggatcga caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat
     7921 cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta ccgcccatta ccattacgac
     7981 gttagcgttt ttgatatcca cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc
     8041 aagacttgct accgttggtc cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc
     8101 gagggagcgg gcaaatttct gtgtcagcat cccggtttcg ttgctggcac cggaggcaca
     8161 cagcataccg gtagaaagcc aacggtttac cgttacgccc tgctcgttct tttcaataaa
     8221 gttagcgtca cggtcagctt tcatcagctt tgcaatacgg gagaatgctt cttcccagct
     8281 aatgcgctgc catttgtcag aacctggcgc acgatattcc gggtagcgca gacggttttc
     8341 gctgttgacg taatccagca aaccggcccc ttttgggcac agcgcaccac ggcttaccgg
     8401 atgatccggg tcaccttcaa tgtgataaat cgcttctctg gcgtttttcg cgccatcacc
     8461 caggctatac atcaatagcc cgcaacctac ggaacagtat gtgcaggtgt tacggatctc
     8521 tttagcgcgt aataatttgt agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc
     8581 caatgccgct accgttgttc cagccatacc gcccgcgcag attttaaaaa attgtctgcg
     8641 actgacgtcc attgctttcc tcttttttca ggggtattac tgcgcgagga aactaacagt
     8701 aaaattctga atctttttgc gcgaaatcaa aaagaacacg tgtttgccac aaaataatcc
     8761 gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac gcaccactgc ctgacaggcc
     8821 agttaaaaaa atgctataaa attcagctta atttttaacg gcaagagaga caaaacagcg
     8881 agcatgacac gacaaaaagc aacgctcata gggctaatag cgatcgtcct gtggagcacg
     8941 atggtaggat tgattcgcgg tgtcagcgag gggctcggcc cggtcggcgg cgcagctgct
     9001 atctattcat taagcgggct gctgttaatc ttcacggttg gatttccgcg tattcggcag
     9061 attccgaaag gctatttact cgccgggagt ctgttattcg tcagctatga aatctgtctg
     9121 gcgctttcct tagggtatgc ggcgacccgt catcaggcga ttgaagtggg tatggtgaac
     9181 tatctgtggc ccagcctgac aattctcttt gccattctgt ttaatggtca gaaaaccaac
     9241 tggctgattg taccaggatt attattagcc ctggtcggcg tctgctgggt gttaggcggt
     9301 gagaatgggt tacattatga tgaaatcatc aataatatca ccaccagccc attgagttat
     9361 ttcctggcgt ttattggtgc gtttatctgg gcagcctatt gcacagtaac gaataaatac
     9421 gcacgcggat ttaacggaat taccgttttt gtcctgctaa cgggagcaag tctgtgggtt
     9481 tactattttc tcacgccaca accagaaatg gtatttagca cgcccgtcat gattaaactc
     9541 atctctgcgg catttacctt aggatttgct tatgctgcat ggaatgtcgg tatactgcat
     9601 ggcaatgtca ccataatggc ggtaggctcg tattttacgc ccgtactttc ctcagcgctt
     9661 gcagccgtgc tgctcagcgc cccgctgtcg ttctcattct ggcaaggcgc gctaatggtc
     9721 tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg gttaaaataa ttgccgggtc
     9781 tacggcccgg cagttaatca gataaattcc gacaatatta aaaaataaat ttcaaatacc
     9841 agtaattcac
//
LOCUS       DAZKGS010000160         9749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKGS010000160 DAZKGS010000000
VERSION     DAZKGS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..966
                     /gene="hlyE"
                     /locus_tag="ACHOQY_003954"
     CDS             55..966
                     /gene="hlyE"
                     /locus_tag="ACHOQY_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFP9679959.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1173..1619)
                     /gene="ycgN"
                     /locus_tag="ACHOQY_003955"
     CDS             complement(1173..1619)
                     /gene="ycgN"
                     /locus_tag="ACHOQY_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9679960.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1711..2370)
                     /gene="ycgM"
                     /locus_tag="ACHOQY_003956"
     CDS             complement(1711..2370)
                     /gene="ycgM"
                     /locus_tag="ACHOQY_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9679961.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2442..2735)
                     /locus_tag="ACHOQY_003957"
     CDS             complement(2442..2735)
                     /locus_tag="ACHOQY_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9679962.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2747..2905)
                     /locus_tag="ACHOQY_003958"
     CDS             complement(2747..2905)
                     /locus_tag="ACHOQY_003958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9679963.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2976..3377
                     /gene="pliG"
                     /locus_tag="ACHOQY_003959"
     CDS             2976..3377
                     /gene="pliG"
                     /locus_tag="ACHOQY_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9679964.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3480..3848)
                     /gene="ycgJ"
                     /locus_tag="ACHOQY_003960"
     CDS             complement(3480..3848)
                     /gene="ycgJ"
                     /locus_tag="ACHOQY_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9679965.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4368..5063
                     /gene="minC"
                     /locus_tag="ACHOQY_003961"
     CDS             4368..5063
                     /gene="minC"
                     /locus_tag="ACHOQY_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9679966.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5087..5899
                     /gene="minD"
                     /locus_tag="ACHOQY_003962"
     CDS             5087..5899
                     /gene="minD"
                     /locus_tag="ACHOQY_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9679967.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5903..6169
                     /gene="minE"
                     /locus_tag="ACHOQY_003963"
     CDS             5903..6169
                     /gene="minE"
                     /locus_tag="ACHOQY_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9679968.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6541..6885)
                     /locus_tag="ACHOQY_003964"
                     /pseudo
     CDS             complement(6541..6885)
                     /locus_tag="ACHOQY_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6920..7039)
                     /locus_tag="ACHOQY_003965"
     CDS             complement(6920..7039)
                     /locus_tag="ACHOQY_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9679969.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(7000..7185)
                     /gene="ymgJ"
                     /locus_tag="ACHOQY_003966"
     CDS             complement(7000..7185)
                     /gene="ymgJ"
                     /locus_tag="ACHOQY_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP9679970.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7286..7459
                     /gene="ymgI"
                     /locus_tag="ACHOQY_003967"
     CDS             7286..7459
                     /gene="ymgI"
                     /locus_tag="ACHOQY_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP9679971.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7461..7805
                     /gene="ymgG"
                     /locus_tag="ACHOQY_003968"
     CDS             7461..7805
                     /gene="ymgG"
                     /locus_tag="ACHOQY_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP9679972.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7809..8144
                     /gene="ymgD"
                     /locus_tag="ACHOQY_003969"
     CDS             7809..8144
                     /gene="ymgD"
                     /locus_tag="ACHOQY_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP9679973.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8201..>9749)
                     /locus_tag="ACHOQY_003970"
     CDS             complement(8201..>9749)
                     /locus_tag="ACHOQY_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9679974.1"
                     /translation="DATNSKITGSANISTDVNTHTYLSLSDNSTWDIKADSTVSNLTV
                     DNSTVYISRADGRDVEPTRLTITENYVGNNGVLHLRTELGDDNSATDKVVINGNTSGT
                     TRVKVTNAGGSGAYTLNGIEIISVEGESNGEFIKDSRIFAGAYEYSLTRGNTEATNKN
                     WYLTNFQATSGGETNSGGSSAPTVAPTPVLRLEAGSYVANLAAANTLFVMRLNDRAGE
                     MRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGAELLTGGFTDSD
                     SWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLYATWFADDISKKGAYIDAWAQ
                     YSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIW
                     MGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIH
                     NSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVM
                     VGLKYKF"
BASE COUNT         2727 a         2308 c         2164 g         2550 t
ORIGIN      
        1 agttgtaaaa caggagtttc attacaattt atatatttaa agaggcgaat gattatgact
       61 gaaatcgttg cagataaaac ggtagaagta gttaaaaacg caatcgaaac cgcagatgga
      121 gcattagatc tttataataa atatctcgat caggtcatcc cctggcagac ctttgatgaa
      181 accataaaag agttaagtcg ctttaaacag gagtattcac aggcagcctc cgttttagtc
      241 ggcgatatta aaaccttact tatggatagc caggataagt attttgaagc aacccaaaca
      301 gtgtatgaat ggtgtggtgt tgcgacgcaa ttgctcgcag cgtatatttt gctatttgat
      361 gagtacaatg agaagaaagc atccgcccag aaagacattc tcattaaggt actggatgac
      421 ggcatcacga agctgaatga agcgcaaaaa tccctgctgg taagctcaca aagtttcaac
      481 aacgcttccg ggaaactgct ggcgttagat agccagttaa ccaatgattt ttcagaaaaa
      541 agcagctatt tccagtcaca ggtagataaa atcaggaagg aagcgtatgc cggtgccgca
      601 gccggtgtcg tcgccggtcc atttggatta atcatttcct attctattgc tgcgggcgta
      661 gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgtgca gaatttcttt
      721 accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc caaattgaaa
      781 ttaaccaccg aaatagccgc catcggggag ataaaaacgg aaactgaaac aaccagattc
      841 tatgttgatt atgatgattt aatgctttct ttgctaaaag aagcggcaaa aaaaatgatt
      901 aacacctgta atgagtatca gaaaagacac ggtaaaaaga cactctttga ggtacctgaa
      961 gtctgataag cgattattct ctccatgtac tcaaggtata aggtttatca catttaaaaa
     1021 cacagacgtt ttttaaagat tgagagttac ctgttgataa taaggtatcg ggtaactctc
     1081 attatgacag agcgctttca aaaacagggc tacgaaatgg cgagcatcca gacgtatatt
     1141 aagaaagccc ccatcaacat ctattaaatt tatcactgcg cccagtcagg tttatttaag
     1201 atatgatcct gccagtcaat cacttctgat tctttcactg cgatatgacg cacagagata
     1261 cgttcaccat gcattgccgc tttcgaaccc gtaagtagcg gatgccacgc aggtaaatct
     1321 ttaccttccg ccagcaaccg ataagcgcag gtcattggca gccattcgaa tgttggcaga
     1381 ttttcacggg ttaatttaat gcagtcgggt tcaaactcga aacgacgttc gtagttccga
     1441 cattgacagg ttttaatatt gagctggcga caggcgacgt tagtgaagta gatttcgtcg
     1501 gtgtcttcat ccatcagttt atgcaggcaa cactgaccgc aaccatcaca caacgactcc
     1561 cattccgcat cgctcatttc gtccagggtt ttactttgcc agaaaggtac atcgctcatc
     1621 aggtgttccg ccattacgtt aaaacgcacc ttataaccag tctggcacag cgatgcaagt
     1681 tttgccgccg ctttcaggcg gcaaaaagta ttacaaaacg cgagttgcca aagaatgccc
     1741 atcgaaagtg actgtcagct catcaccgct ttgcaacggg ccgacgccat caggcgtgcc
     1801 tgtcagcaca acgtcaccgg ccttgagggt aaaaaacttg ctcatataag cgatcagcgg
     1861 aacaatttta tggatcatgt ccgcagtcgt accttgctga cgttgctcgc cgtttacgct
     1921 caggcccagc gttgtatttt gcgggtcgcc ggtaaattcc gccgcgggaa taaacccgga
     1981 aagcggacaa gagttatcaa acgctttagc cttttcccac ggctgcccgg ctttcttcat
     2041 ttttccctga acatcacgca atgtcagatc gagcgccacg ccataaccgg caatggcttt
     2101 gcggacatgc tcttccgtag cctgacgcag cgtcgcgcca atcaacaccg ccagttcgac
     2161 ttcatgatga actgaaccga aatcggatgg gatcgccaat ggctgccgca gatcgcacag
     2221 tgccgtttct ggtttaataa acagcactgg ctcttcgggc actgcgctgc ccatctcttt
     2281 aatatgtttg gcatagttac tgccaacaca gactacttta ctcaccggat aatccagcag
     2341 cgcaccttgc cagttgtgat gttgatacat aacgttcccc tcaccttatc gtggatatga
     2401 aaacccgcgc cgacaaagat aatgccaccg ccggatatcg gttatttgtt agtgtcgtct
     2461 gttttctgcc ccatgacgga aagatgttgc ttcagcaaat cttcgggtgg tggcggtaac
     2521 tgcaaatagt aaccttgctc ggttaatgcc agtttaacct tttcaatatc agcattgacc
     2581 agtttcttcc gcccatccaa cggcagaatc atcgctaact gaggctgacc aaaacctttc
     2641 atcagttcct caggaacacg cgaaaaatcg tctttttttt cgacatataa ataggtctgg
     2701 tcacgcttgc tgcttcgata aatcacacaa aacatacttt tactctttaa gatgccaggt
     2761 tttggcataa gctaacatca atgaaacccg cgccacaagc cagccagctg tagatttcta
     2821 cggtttgttg agtccatgcc cctttataag taatcccccc ggcaaaaatg ccgcgccggg
     2881 attttaccga acgcaggaaa gccactggaa ttctcccgtt agggattgtt atattataac
     2941 agttcatcgt actcattctg aacaggagac taccaatgaa aatcaagagc atcagtaagg
     3001 ctgtattgct gtttgcactg ctaacctcca cgagctttgc tgcgggtaag aatgtcaatg
     3061 ttgagttcag aaaaggccat agcagcgctc agtactccgg cgaaataaag ggatacgatt
     3121 acgatacata taccttctat gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg
     3181 aaggcgctga tacttacctc ttcgggccag gaatcgatga ttcagttgat ttatccagat
     3241 attccccaga actcgatagt cacggacaat actcactccc tgcttctggc aaatatgagc
     3301 tgcgagtgtt gcaaacccga aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg
     3361 atattcaaat taagtaaatg tcaaccgacg gggactctcc ccgtcataaa cgttaacgcc
     3421 ccactcctct ctgtcgctca agaggttaat gacgaataga acataatgta aacaagacat
     3481 cactcacgac acataaataa catctttgtg gtggtttgat tgatttttcc actccgctta
     3541 ccatctacac caaaataacg atcatcacga cataattttt ctttaacatc gcaaaataca
     3601 ccgttagcga atgtaaattc gctgggatcg tagccttgta atgattgtaa gttttcagcg
     3661 gctttttcgc cgagatattt ccttgttaac tctggggaaa tgccatctga gttcacacaa
     3721 atatacgggt cgcaaacaac gccgcacgcc ggagattgaa gtccgctagc gtcatgtcca
     3781 gacgccattg atgagaacat catccccaca cctgacaatc cgatataaat aatacgcatc
     3841 atattcatag cacatcctga taatccaacc gtacaacact tatgctcatt tcgtcatcaa
     3901 tccgtaatca aaaccgctga cctggacgag ttacgatgac ctgacagaag tatcagacgt
     3961 cttctgcggt ttctatgata gagcaattct gtggcggata ttaagataac gggtatctgt
     4021 tctttaatga tgccgcaaaa aatggaaagt taagaaagcc tggcgtggtg aaaaaataca
     4081 cttgcctcaa tactggatat ttaaacaggt ctggagatgc tgtcacgatt agggtgcgga
     4141 aaaaggaagc gtaaataaga agaatcaaaa gtacgacggc aatgggttga ttgacaaggg
     4201 tattttttaa gctatgaatc agcgccattt atcacagaat agacttttac tctgaataaa
     4261 tgggagggtg acttgcctca atataatcca gactataaca tgccttatag tcttcggaac
     4321 atcatcgcgc gctggcgatg attaatagct aattgagtaa ggccaggatg tcaaacacgc
     4381 caatcgagct taaaggcagt agcttcactt tatctgtggt tcatctgcat gaggcagaac
     4441 ctaaggttat ccatcaggcg ctggaagaca aaatcgctca ggcccccgca tttttaaaac
     4501 atgcccccgt tgtactcaac gtcagtgcac tggaagaccc ggtaaactgg tcagcgatgc
     4561 ataaggcggt ttcggcaacc ggtttgcggg ttattggcgt aagtggctgc aaagatgcgc
     4621 aacttaaagc cgaaattgaa aaaatggggc tgcctatcct gacggaagga aaggaaaaag
     4681 cgccacgtcc agctcccgca ccgcaggctc cagcgcaaaa tacaacgccg gtcacaaaaa
     4741 cgcgtttaat agataccccg gtgcgttccg gtcagcgtat ttatgctcca caatgtgatc
     4801 tgattgttac aagccacgtt agcgctgggg ccgaattgat tgccgatggt aacattcatg
     4861 tctatggcat gatgcgcggt cgtgcgctgg caggggcaag tggtgaccgg gaaacgcaaa
     4921 tattttgtac gaacctgatg gcggaactgg tgtccatcgc aggtgaatac tggctgagtg
     4981 atcaaatccc agcagaattt tatggcaaag cggcgcgact gcagttagtc gaaaacgctt
     5041 tgaccgttca accgttaaat tgatcccttt ttaacaagga atttctatgg cacgcattat
     5101 tgttgttact tcgggcaaag ggggtgttgg taagacaacc tccagcgcgg ccatcgccac
     5161 tggtttggcc cagaagggaa agaaaactgt cgtgatagat tttgatatcg gcctgcgtaa
     5221 tctcgacctg attatgggtt gtgaacgccg ggtcgtttac gatttcgtca acgtcattca
     5281 gggcgatgca acgctaaatc aggcgttaat taaagataag cgtactgaaa atctctatat
     5341 tcttccggca tcgcaaacac gcgataaaga tgccctcacc cgtgaagggg tcgccaaagt
     5401 tcttgatgat ctgaaagcga tggattttga atttatcgtt tgtgactccc cggcagggat
     5461 tgaaaccggt gcgttaatgg cactctattt tgcagacgaa gccattatta ccaccaaccc
     5521 ggaagtctcc tcagtacgcg actctgaccg tattttaggc attctggcgt cgaaatcacg
     5581 ccgcgcagaa aatggcgaag agcctattaa agagcacctg ctgttaacgc gctataaccc
     5641 aggccgcgta agcagaggtg acatgctgag catggaagat gtgctggaga tcctgcgcat
     5701 caaactcgtc ggcgtgatcc cagaggatca atcagtattg cgcgcctcta accagggtga
     5761 accggtcatt ctcgacatta acgccgatgc gggtaaagcc tacgcagata ccgtagaacg
     5821 tctgttggga gaagaacgtc ctttccgctt cattgaagaa gagaagaaag gcttcctcaa
     5881 acgcttgttc ggaggataag ttatggcatt actcgatttc tttctctcgc ggaagaaaaa
     5941 cacagccaac attgcaaaag aacggctgca gattattgtt gctgaacgcc gtcgcagcga
     6001 tgcagaaccg cattatctgc cgcagttgcg taaagatatt cttgaggtca tttgtaaata
     6061 cgtacaaatt gatcctgaga tggtaaccgt acagcttgag caaaaagatg gcgatatttc
     6121 tattcttgag ctgaacgtga ccttaccgga agcagaagag ctgaaataag cccgctgtaa
     6181 aaacgcattt atcttcaagg cagagtcatt tctgccttga gtttttcatc cctctcatcc
     6241 acgttgtggt aaagcggcga gtattcttgc tgatactcct cattgctatt tccacctccc
     6301 cctttacacc ttaaggctgt tcccacgcac agaacaaaat gtttatgcct ggttgagtaa
     6361 ataacctcat tgttagtgtg gatacactgg gggatgataa ccccgcaatt tcattattca
     6421 ttgttctccc ataggatgag gtttcccgcc gtaactggcg ggcttttttt gccccagaat
     6481 tttccctttt agccccctgt aaacgaaaac tgcgccgaag cgcagtttaa tcgttgatgg
     6541 tcagaacgac cagttcacac cagccaccgc attccacggg gattccacac cggcaccatg
     6601 gctatacccc accccgaaat gcccgcttaa cgtactgctg aatgaggctt taatacctgc
     6661 ctggtatatt ccacgtctgc ccgacaaatc attgacaaaa ttaccgtcac tattcacttt
     6721 cacccgatta tcatcgacaa attctttgct cacagccgcc ttcaggcagg gttcaatttc
     6781 cataccgttc cccagacgca tgttgtaact cagcgttgca cccagttcac gatatatact
     6841 gcgggtatcg actgatttcg attccatgcc attggataaa tgatagtagc gtctaagtga
     6901 cctaagctct gctgcaacat cagcccgcag gcaccagacc aggggattca tcctgaagag
     6961 acagcgcagg tgtattgtgt tcaccgctca ccaaagacat catgatgaaa tgatgatatc
     7021 ccgcataaga aagaggcatt ttttacacgc agtgcgctga agtgtagttg gataaaaaat
     7081 cactcttttc agataatact gtcgtacgct tatctttcga caatgaggcc gtcggcaaaa
     7141 taaaatgact aatgtaatcg ttgctgatga atatcttctg ctcacataaa aatcacacaa
     7201 taatattgat aaatcagatt atcttacctt tacaacattg cgttaacagt caatgacact
     7261 tcttgaataa aaatattgtc aatcaatgag ttattcctca tttaagatta ttctcattca
     7321 cgtaaaaaac ataattccta tcatcacagc cacgctcatt ttgaattatc tgaataatag
     7381 cgagcgttct ttagttaagc agatcttaat tgaagacgaa attattgtgt gtgcaactta
     7441 cttaataccg gacatctaag ttgaagaaaa aaatacttgc cttcgggctt atctcagcat
     7501 tgttctgctc cacgccagcg atggcggata tgaatcgcac cacgaaaggt gcattgctag
     7561 gcgcaggcgt tggtttactt actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg
     7621 ttggcgctgg tgttggtgca gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag
     7681 gtgcaaaagt aggtgccgcc gttggtgctg tcactggcgt tctgaccggc aatggcctcg
     7741 aaggggcgat taaaggtgcc gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga
     7801 aataaggaat gatgatgaaa aaatttgctt tactggcggg cctgtttgtt ttcgcaccaa
     7861 tgacctgggc gcaggactac aatattaaaa acggtttgcc gtcagaaacc tatattactt
     7921 gcgcagaagc caacgaaatg gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg
     7981 taatgggtaa tgccagcgtt gccagccgtg atttaaaaat tgagcaatct ccggaactga
     8041 gcgccaaagt ggtagaaaaa ctgaatcagg tttgcgctaa agacccgcaa atgctgttga
     8101 tcacggcaat tgatgacacc atgcgtgcta tcggcaaaaa ataatatttc atatgtaccg
     8161 cgtcgatgaa tacgacgcgg ttttttagct aacggcggga tcagaactta tatttcaggc
     8221 ccaccatcac tgcggtgtca ttgtagccat tatcacccag ctgcacgccc acattccccc
     8281 agacgctggc cgctggattc aggttgccat ttacgccaag tttaatttcc cccaaattac
     8341 tcgccccatc ttgttcgact ttcacaccgt tcattgagac agcaaaatct ttactgttat
     8401 ggatccagtt catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct
     8461 cctgagtacg aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat
     8521 tctcaatacg tgagccattg gcttcggtgt gcgcattatg atcgacgccc atccagatag
     8581 cctgtgcctg tggctggaat atccacgtgt atttcgccgc ttccagacca aaggatttat
     8641 tcagggcaaa gccgtaaccc gcttccagcg aaacggttgc gcctttcgca ctataggatt
     8701 cataggctaa ttcatcccct ttcaccgagt ttttaaacca gctatattgc gcccaggcgt
     8761 caatgtatgc gcctttttta ctgatgtcat cggcaaacca agtggcatac agccctgcgc
     8821 aatagcctct gacactccct ttcgaacgat aatccgacac gctggaatga gttgagttgt
     8881 agtcgcgggc ataaccagcc atcactccca aacgccaact gtcactgtcg gtaaaaccac
     8941 cggttaacag ctcggcccct aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc
     9001 cgttgctgtc acgccaggca ttatgcccgc caatttgacg tagccaaagt cggcttgaac
     9061 gctcctgttc agttacagga tcgatgtagc gcatttcacc cgcacggtcg ttcagacgca
     9121 taacaaaaag agtgttagcg gctgccaggt tggcgacgta acttccagct tcgaggcgca
     9181 ggacgggggt aggcgcaaca gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc
     9241 tcgttgcctg gaagttagtc agataccagt ttttattggt cgcttcggta ttacctcggg
     9301 ttaatgaata ttcgtaggca ccggcgaaaa tcctcgaatc cttaataaat tccccatttg
     9361 attccccctc aacgctgata atctctatcc catttaacgt gtaagctccg ctgcctcctg
     9421 cattggtaac tttaactcgg gttgttccag aggtatttcc attaataacg actttatccg
     9481 tagctgaatt atcatcaccc aattcagttc tgagatgcaa tacgccatta ttaccaacgt
     9541 aattttcagt tattgttaat cttgttggtt cgacatcccg tccatctgcc cgggaaatat
     9601 agaccgtact attatcaacc gtgaggttgc tcaccgttga gtcagcttta atatcccagg
     9661 tactattatc tgaaagcgac agataggtat gagtgttaac atctgttgaa atattagctg
     9721 aacccgttat ttttgaatta gttgcatcg
//
LOCUS       DAZKGS010000161         9730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKGS010000161 DAZKGS010000000
VERSION     DAZKGS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..251)
                     /gene="cspA"
                     /locus_tag="ACHOQY_003971"
     CDS             complement(39..251)
                     /gene="cspA"
                     /locus_tag="ACHOQY_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9679975.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(532..822)
                     /locus_tag="ACHOQY_003972"
     CDS             complement(532..822)
                     /locus_tag="ACHOQY_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9679976.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1256..1966
                     /locus_tag="ACHOQY_003973"
     CDS             1256..1966
                     /locus_tag="ACHOQY_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9679977.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2016..2990)
                     /gene="ghrB"
                     /locus_tag="ACHOQY_003974"
     CDS             complement(2016..2990)
                     /gene="ghrB"
                     /locus_tag="ACHOQY_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9679978.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3094..3753)
                     /locus_tag="ACHOQY_003975"
     CDS             complement(3094..3753)
                     /locus_tag="ACHOQY_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9679979.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3906..6239
                     /gene="bisC"
                     /locus_tag="ACHOQY_003976"
     CDS             3906..6239
                     /gene="bisC"
                     /locus_tag="ACHOQY_003976"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9679980.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6208..6648)
                     /locus_tag="ACHOQY_003977"
     CDS             complement(6208..6648)
                     /locus_tag="ACHOQY_003977"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9679981.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6645..7208)
                     /gene="tag"
                     /locus_tag="ACHOQY_003978"
     CDS             complement(6645..7208)
                     /gene="tag"
                     /locus_tag="ACHOQY_003978"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9679982.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7366..8064
                     /locus_tag="ACHOQY_003979"
     CDS             7366..8064
                     /locus_tag="ACHOQY_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9679983.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8293..9495
                     /locus_tag="ACHOQY_003980"
     CDS             8293..9495
                     /locus_tag="ACHOQY_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9679984.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2316 a         2364 c         2514 g         2536 t
ORIGIN      
        1 atggcaggga tcttagattc tgtgctttta agcagagatt acaggctggt tacgttacca
       61 gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca ctttctgacc ttcgtccaga
      121 gatttgtaac catcgttctg gatagcagag aagtgtacga acacgtcttt agagccatcg
      181 tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc attttacgat accagtcatt
      241 ttaccggaca tagtgtatta cctttaataa ttaagtgtgc ctttcggcga tatggcgtgc
      301 tttacagatt ttgaagcgtt aaaggaatgt gcactacgag gggtatcaac gataactctt
      361 gaagggactt gccttactac actgctttaa tggtctgtac gtcaaaccgt tgatgtgcat
      421 taagccacgc attggcgggt gatgcaacaa ttatttttca tatttatgat taatcggttg
      481 gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag tggataaaag tctattccat
      541 caactgctta cttaatgccg ggttggcttg aatcaaacgc atcaatttta gttcggcact
      601 tgaaggcttc acgcgtctgg attcccattc ctttaccatg gcgactgata cgcccaaaac
      661 ccgggcgaaa tcatcgattt ttaaccctgt cccttttcgt aactgctcaa tttcggtaca
      721 ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca tctttaaaaa caatctgttc
      781 caggctgctc aacagctcat gcattggatc tttatattcc attgaaaact cctcaaatca
      841 cactgcggga tcgtgaattg catcgaagct cattaagaat agtcgggaaa agcagacaca
      901 gcggggtgaa gtcagggcat gctctggcta tgattcgatt cagccagtca tttagcgtga
      961 cttatagcca cctaacgatc tgataaatgc cgatatatct cttaccactc ctggtttgta
     1021 aagatttcga aaatctaacc ttctgcgaag gcgtaaatct ctggctacaa aaaaagcaac
     1081 ggcactatca ttttttaaca atgaaacaac aaattaacct tgtttcattt tctccatgat
     1141 tttaccagga agattacgta gaaagggtat cctgcaagcc tgtcctggac tatccttgtc
     1201 acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt gaaaggagta agaaaatggc
     1261 gacaggaaag tcctgctctc gctggtttgc gcctcttgcg gcgttattaa tggtagttag
     1321 cctgagtggg tgttttgata aagaaggcga tcagcgtaaa gcgtttatcg acttcctgca
     1381 gaatacagtg atgcgtagcg gtgaacgtct accaaccctg actgccgatc agaaaaaaca
     1441 gtttggtcct tttgtctctg attacgcgat tctgtatggt tattctcagc aggtgaatca
     1501 ggcgatggat tccggtctgc gcccggtcgt agacagcgtt aacgcaattc gcgtaccgca
     1561 ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaac ggttcgctgg gtgtgctggc
     1621 gcaacaactg caaaatgcga agttgcaggc cgatgctgcg cactctgcgc taaaacagac
     1681 cgatgatttg aaaccggtct ttgatcaggc attcaccaaa gtggtaacga cgccagctga
     1741 tgcattgcaa ccgttgattc cggctgcaca aactttcacg caacaactgg tgatggttgg
     1801 agactacatt gctcagcaag gtactcaggt gagctttgtc gcaaatggca tccagttccc
     1861 gacttcacag caggcaagtg aatataacaa actgattgcg ccattaccgg cacaacatca
     1921 ggcgtttaat caggcctgga ctacagcagt tactgcaacc caataaagag taaaagcccg
     1981 agcgattatc gggctttact tcacgcagtc gcggcttagt ccgcgacgtg cggattcaca
     2041 cagttcttct caacctttcc ttgtaacgca tcaatcaaat tatccacggc acaggcggcc
     2101 atgccataac gcgtctcatg ggtggcagat ccaatatgcg gtactgcgac gacgttggcc
     2161 attgagagca acggcgaatc tacggacagt ggctcttgtt cgaagacatc cagcccggcg
     2221 gcgtgaattt cgcctttctg caatgcagca atcagtgcat tttcatcaac caccgggcca
     2281 cgtccggcat taatgaaaat ggcggaggat ttcattttgg cgaattgttc tgcgccaaac
     2341 agatgatgcg tctcatcagt taacggcagg atcaggcaaa cgaaatctga ctcttgtaac
     2401 agagtatcca aatcgcagta gcgggcgttg aagcgttctt ctgcttcttt atggtggcgg
     2461 cgcgcgttat agaggatggg catgttgaag ccaaagtgcg cacgttgtgc cagcgccata
     2521 ccgatccgtc ccatcccgac aatgcccagt gttttatggt gaacgtcagt gccgtaccag
     2581 tccgggccta tgctcgcggt ccattcgcct gcttttaccc gttctgctac ctccacaacc
     2641 cgacgagcgg tagacaacac cagcgccatc agcgtatcgg cgacggtttc tgttaatacg
     2701 gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg catcgacatc aaaattgtca
     2761 tagccgacgg agatcgttga tgtggcacgc agtttcggca ttttttccag caatgcggca
     2821 tcaacattct cgtttgaacc cagtaaacct tcagcttcgg caaaaattgc tgcattttgt
     2881 tcgacggttt gtgggctgag gtttgccacc tggtgaacgg tgaaatgctc ttgcaggcgt
     2941 tgcagtaaat catcaggtaa ggctttgtag aggataacgg acggcttcat gcttctctcc
     3001 attcactgat aaggcgatca ccttagcctg aaatatgatg aaggaaaact gaagaatgcc
     3061 aggtgatttc tgcatcacct ggcatgaaag ggattacagc gggcttaagg taatttctac
     3121 acggcggttt tgcgccttac cttctgcggt gctgttgctg gcgattgggt tagccgggcc
     3181 aaggccctga gtacggatgc ggctggcgtc cacgccctgg gtgatcaacg cgctggcaac
     3241 ggaatccgca cgttgctggg agagacgcat gttcaggtcg tgaccacccg tgctgtcggt
     3301 ataaccaatc acgttaaccg ccgttttcgg atactctttc agcaccattg ccacgccggt
     3361 cagggtgtta gcgcccgcag gtttcagggt cgcgctgctg ctgtcgaagg tcacattgtt
     3421 cggcatattg aggataatgt tatccccgct gcgggttacg ctaacaccag tgccgcgcat
     3481 tttgtcacgc agcttcgctt cctgcacatc catgtaataa cccacgccgc cgcccagagc
     3541 tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga tctttcttcg aagaagagag
     3601 cgcaccaata cccgcgccca cgagagagcc cagacctgcg ccgatagcag atttacctgc
     3661 ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat accgccagag cgccactcac
     3721 tacggcggca ataagataaa cacgtttctt cattgttaat ccttaataac ctttttattc
     3781 tttgccacgg gttccgtggc gggagattat gccgcgtgaa catgaagatt attcctggga
     3841 atactcggaa atttgtaagt aatatttaac tgctcaatac atctaacctt tcaggagttt
     3901 tcggtttggc caactcatcc tcacgatatt ccgttctgac tgccgcccat tgggggccca
     3961 tgctggttga aaccgacggc gaaaccgtgt ttagctcgca tggcgcgtta gccacaggaa
     4021 tggaaaactc cttgcagagc gcggttcgcg accaggttca cagcaataca cgggtacgat
     4081 ttccaatggt gcgcaaaggc tttcttgcgt caccggaaaa tccgcaaggc attcgtgggc
     4141 aggatgaatt tgttcgcgtg agttgggatg aggcgctgga tcttattcac caacaacata
     4201 aacgcattcg tgaggcttat ggtccggcat cgatttttgc tggttcctac ggctggcgtt
     4261 caaacggcgt gctgcataag gcctcgacat tattacaacg ctatatggcg ctggcaggcg
     4321 gttataccgg gcatctgggg gattattcga ccggcgcggc acaggcgatc atgccgtatg
     4381 tcgtgggtgg tagcgaagtt tatcaacagc aaaccagttg gccgctggtg ctggaacata
     4441 gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac gctgaaaatt gcgtggaatg
     4501 catctgatga gcaggggctt tcttactttt ccgcactgcg tgacagcggg aaaaagctga
     4561 tctgtattga tccaatgcga tcggaaaccg tcgatttctt tggcgataaa atggagtggg
     4621 tggcaccgca catgggcacc gatgttgcgc tgatgctggg gatcgcccat acgctggtgg
     4681 aaaatggttg gcacgacgaa gcgtttctga cgcgttgcac cacaggttat gccgtcttcg
     4741 cctcttattt gctgggcgag agtgacggaa tagcgaaaaa cgccgaatgg gcggcagaga
     4801 tttgtggggt taacgcagcg aaaatccgcg agctggcggc tcttttccac caaaacacga
     4861 ccatgctgat ggctggctgg ggaatgcagc gccaacagtt tggtgagcaa aaacactgga
     4921 tgatcgtcac gctggcggca atgttggggc aaatcggcac acccggcggc ggttttggtc
     4981 tttcttatca ttttgccaat ggtggtaacc ccacgcggcg ttctgcggtg ctctcttcca
     5041 tgcagggcag cttgccgggt ggctgcgatg cggtggataa aatccctgtt gcccgcattg
     5101 ttgaagcact ggaaaaccct ggtggcgcat atcaacacaa cggtatgaac cgacatttcc
     5161 cggatattcg ttttatctgg tgggcgggcg gtgccaactt tactcatcat caggatacca
     5221 atcgtctgat ccgtgcctgg caaaaaccgg agctggtggt gatctctgaa tgcttctgga
     5281 cggcggcggc gaaacacgcg gatatcgttc tgcctgcgac tacctctttt gagcgtaatg
     5341 atctcaccat gaccggtgat tacagtaatc agcatctggt gccgatgaag caagtggtgc
     5401 cgccacgcta tgaagcgcgt aatgattttg atgtttttgc cgagttgagt gaacgttggg
     5461 agaagggcgg ctatgcgcgg tttacggaag gaaaaagtca gctgcaatgg ctggaaacgt
     5521 tttataacgt tgcccggcag cgcggggcaa gccagcaggt tgaattgccg ccatttgctg
     5581 agttctggca agccaaccag ttaattgaga tgccggaaaa cccggacagc gagcggttta
     5641 ttcgcttcgc tgatttttgc cgcgatccgc tggcgcatcc gttaaaaacc gccagcggca
     5701 agattgaaat cttctcacag cgtattgccg attacggtta cccggattgc cctgggcatc
     5761 caatgtggct ggagccggac gaatggcagg gcaatgccga accagaacag ttgcaggtac
     5821 tttctgccca tccggcgcac cgcctgcaca gccagctgaa ttacagttct ctgcgcgaat
     5881 tgtacgcggt agcaaaccgt gagcctgtca ccattcatcc tgacgatgct caggcgcgcg
     5941 gcataaccga aggggatatg gtgcgggtgt ggaactcccg tgggcagatc cttgccggag
     6001 cggtcattag cgagggaatt aaacctggcg tgatttgcat tcacgaaggg gcatggccgg
     6061 atctggattt aaccgctgac ggtatttgta aaaacggcgc agtgaacgtg ctgaccaaag
     6121 atctccccag ctcgcggctg gggaatggct gtgcgggtaa tacggcgctg gcatggctgg
     6181 aaaaatacaa cggtccggaa ctgacactta cagcgtttga accaccggcc agctcataat
     6241 ccaggtgggt agttgggttt catcctgcca tgcgcaatcg acaatgtgaa aaccctgcgc
     6301 acggtaaaaa tctatcgccg gttgattttt ttgataaacc tccagcatca gatggggata
     6361 gcgctgctgc acatactgca tcagcgcctt accaataccg cgtctgacgg ccttcggtgc
     6421 gacaaacatt gctgccagaa atcggccttc cataatgctg acaaaaccga gaagcttacc
     6481 gtcctcttcc cagacccagt tttgcgcgtt ggcaagatag gcatcccgca ccagcggaat
     6541 gcagtcacgc cagtaattcg cttttataaa gggatgcccc caggttgtac tttccagcca
     6601 cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc cgaatcatgg tttatttccc
     6661 ggatagcaac agcagccaac cacatgatca ttcaccagcc cacatgcctg cataaaggag
     6721 taacagattg tggtgccgac aaacttaaaa ccacgttttt tcagtgcctt agatagggcg
     6781 tcggaggcgg acgtagatgt gggaatttcg ctcaacgttg tggcttgtgt cacctgtggc
     6841 tgatgattta caaacgacca gacaaagtcg gcaaacggtt cgccgttctg ttccatttgc
     6901 aggtacgccc gcgcattacc aataattgcc tgaattttcc ctcgatggcg gataatcccg
     6961 gcgtcctgta ccagtctttc gacatcctct tcctgcattg ctgcgacctt caccggatcg
     7021 aactgatgaa agcaggcgcg atagttttcg cgttttttga ggacggtgat ccacgataac
     7081 ccagcctgct gcccttcaag gcagatcatt tcgaacagtt ttttactgtc agtttcaggc
     7141 acgccccatt cattatcatg gtaggcaata taaagcgggt cctgactcac ccagccgcaa
     7201 cgttccatac tttccctcgc tatgtacccg atcgtaaaaa tttcagccgc ttttcttgac
     7261 gcatcggtga aaaagtcaat atttaataca gggctactat gcccgatatc cttcatgcaa
     7321 tgacaataat attgccgaat tcggctcttc cctggagtcg tgttaatgat cataaaaaaa
     7381 agcggtggtc gctggcagct aagcctgctg gcgagcgtgg taatcagtgc cttttttctc
     7441 aacacagctt acgcctggca acaagaatat atcgttgata cgcaacccgg acattccaca
     7501 gagcgttaca cctgggatag tgatcatcaa cctgattaca acgatatttt gtcgcaacgt
     7561 attcaaagta gccaaagggc gctgggactg gaagtcaatc tggcggaaga aactcctgtg
     7621 gatgtgacca gcagtatgag tatgggctgg aattttcctt tatatgaaca ggttacaacc
     7681 ggcccggtcg cggcattaca ttacgatggc acaaccacct cgatgtataa cgagtttggc
     7741 gacagtacta ccacgctgac cgatccgtta tggcatgcca gcgtgagtag cttaggctgg
     7801 cgtgttgact cccggcttgg cgatctccga ccctgggcgc aaatcagcta taaccagcaa
     7861 tttggcgaga atatctggaa ggcgcaatca ggcctgagcc ggatgacggc gacaaaccag
     7921 aacggcaact ggctggatgt caccgtaggc gctgatatgt tgctcaatca aaatattgcc
     7981 gcctatgccg cgctaactca ggcagaaaat accactaata atagcgacta tctgtatacg
     8041 atgggggtta gcgccagatt ttaacgtaac agtcacaatt taaaccattg aataacaaca
     8101 gttgtggcga tagtgggtgc taacttacca aataataaat ttggtgaata attgtcgcgt
     8161 cattcattcc tgaactaagg catttcattc cgttctgatg gcatttcatg ccgtttttcc
     8221 ccaggcataa aatgcacttc gttatggttg tcggcagaga tttttccttt ttattactgc
     8281 aggaatactg ccatgacacc ttcaaattat cagcgtaccc gctggctgac actcatcggt
     8341 actatcatta cccagtttgc actggggtcg gtttatacct ggagcctgtt taatggcgcg
     8401 ctttctgcca agctggatgc gccggtaagc caggtcgctt tctctttcgg cttgttaagt
     8461 ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc aggaacgttt tggcgttaaa
     8521 cgcgtcacca tggcttccgg cattttgctg ggattaggct tcttcctgac cgcgcattcc
     8581 aacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg tcggtctggc ggatggcgcg
     8641 ggttacctgc tgacgctctc taactgcgtg aagtggttcc cggagcgtaa aggtctgata
     8701 tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg gtttcaaatt tatcgacacg
     8761 cagctgctgg aaacggtggg tctggaaaaa acctttgtga tttggggagc gattgcgctg
     8821 gtgatgattg ttttcggcgc aacgttaatg aaagacgcac caaaacagga agtgaaaacc
     8881 agcaatggtg tggtggagaa agattacacg ctggcagagt cgatgcgtaa accgcagtac
     8941 tggatgttag cggtaatgtt cctgaccgcc tgcatgagcg gcctgtacgt gattggggta
     9001 gcgaaagata tcgcccaaag tctggcacac cttgatgtgg tttccgcagc caatgcagtc
     9061 actgttattt ccatcgccaa cctttcaggt cgtctggtgc tgggtattct gtctgacaaa
     9121 atcgcccgta tccgtgttat caccattggt caggtgatat cgctggtggg tatggcggcc
     9181 ctgctgtttg caccattgaa tgcagtgacg ttctttgcag cgattgcctg cgtggcattt
     9241 aactttggcg gcactattac cgtctttccg tcactggtca gtgagttctt tggcctcaat
     9301 aacctggcga aaaactacgg tgtgatttac ctcggtttcg gtatcggtag catttgtggt
     9361 tcgattatcg cctcactgtt tggcggcttc tatgtgacct tctacgtgat tttcgccctg
     9421 ctgattctgt cattggcgct ttctacgaca attcgtcagc cagagcagaa agtattgcgt
     9481 gaggcgcata tttagaaata tgttgctact gaaataaatg cgaggaggga gacttcaaag
     9541 tctccctttt tttatggacg catattaaaa gaacaataat atttacacca caaatatatt
     9601 ttatttgttt atatatcatg taaatataaa tatattccta aaaaaatagt cgttggaagc
     9661 gtttttgtgt gcaattaatt gaagttgaag tacatttcaa tttattgcaa aaactaatct
     9721 tcagtaaaaa
//
LOCUS       DAZKGS010000162         9617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKGS010000162 DAZKGS010000000
VERSION     DAZKGS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..603)
                     /gene="mocA"
                     /locus_tag="ACHOQY_003981"
     CDS             complement(25..603)
                     /gene="mocA"
                     /locus_tag="ACHOQY_003981"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9679985.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            706..1476
                     /gene="yqeC"
                     /locus_tag="ACHOQY_003982"
     CDS             706..1476
                     /gene="yqeC"
                     /locus_tag="ACHOQY_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9679986.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1524..3149
                     /gene="yqeB"
                     /locus_tag="ACHOQY_003983"
     CDS             1524..3149
                     /gene="yqeB"
                     /locus_tag="ACHOQY_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9679987.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3190..4122)
                     /gene="arcC"
                     /locus_tag="ACHOQY_003984"
     CDS             complement(3190..4122)
                     /gene="arcC"
                     /locus_tag="ACHOQY_003984"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9679988.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4170..5555)
                     /gene="hyuA"
                     /locus_tag="ACHOQY_003985"
     CDS             complement(4170..5555)
                     /gene="hyuA"
                     /locus_tag="ACHOQY_003985"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9679989.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5608..6819)
                     /gene="ygeY"
                     /locus_tag="ACHOQY_003986"
     CDS             complement(5608..6819)
                     /gene="ygeY"
                     /locus_tag="ACHOQY_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9679990.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6877..8073)
                     /gene="dpaL"
                     /locus_tag="ACHOQY_003987"
     CDS             complement(6877..8073)
                     /gene="dpaL"
                     /locus_tag="ACHOQY_003987"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9679991.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8131..9318)
                     /gene="ygeW"
                     /locus_tag="ACHOQY_003988"
     CDS             complement(8131..9318)
                     /gene="ygeW"
                     /locus_tag="ACHOQY_003988"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9679992.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2244 a         2341 c         2517 g         2515 t
ORIGIN      
        1 aacgcaatcg tttaactaca ttttctaaat tttagtatac cttttttgcg ctgtaataaa
       61 gtcgtccgga gtatcaatat ctaaaattat ttctgcattt tctatctcga cggaataatg
      121 agctcccata aggagtgcct gacgcatatt ggtgacattg ggtcgttgga ttgcctgcat
      181 caagcatggt tttgacacta aaatcggatg gccggggatg ccattatgga gtggcagtat
      241 tgcgccatca tttcgtaacg accagatttt tctaaaaata tcgatggtga gggttggcat
      301 gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg ggtactgcgg cctttactga
      361 tgttagtaaa ccctgcgcat aatctgggtt gtggataata gtgatattgc tctggttcgc
      421 ataacgttcg tgcagttcat taccacgata gccggtgact aaaataattc ggctacaaaa
      481 ctgcaacgca tttttgatac ttgtatcaag aattgtcccc tgttgccagg gtaacatcat
      541 tttccattgc cccattcttg atgataatcc ggcagcggta attatacagt cgatggctga
      601 catagtggtt ccattattta taggggctaa taacgcctga taattgcgtt acttcctgat
      661 ggattaagtt ggggataatt tatcccagag aggtcataaa gactcgtgaa aagtatagtt
      721 gacccatcgg cattagtcat tgatttaggt gcgcagaaac gtcccactgt gatttctgtt
      781 gttggtgctg gggggaaaac cagcttgctt ttttggctgg cagaactgct ccaggctagc
      841 ggcaggcgtg tgttaatcac gacgacgaca catatgttta tgccaacgtc tcactggccc
      901 gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt ctcttacatc ccccatttca
      961 ttttgttttc acagctggaa agcgaaccag ggaaaagtgc agggatttac gccagaagcg
     1021 attgatgcac tggtgcaacg accagagtgt gacgtaattc tcattgaagc agatggctcg
     1081 cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt gcatacccaa aagcagttgc
     1141 tgcgtgattg ctgtgatggg aggacatact ttgggcgcga aagtgagcac agaaaatgtc
     1201 catcgctggt cgcagtttgc tgatattacc gggttaacac ctgatgcaac cttgcaactg
     1261 agcgatctcg ttgcgctggt tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt
     1321 tgtcggcgag tctggttcat taaccgtttt tctcaatgtg agaatgcgat tgcacaaagc
     1381 gagctccttc aaccgctgca acaacacgac gtagaggcaa tctggctggg cgatatacaa
     1441 gaacatcctg caatcgcgcg cagatttgtg aattagcggc ccgggtattc cgggttactg
     1501 aaaggatatc cattgaggtt ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc
     1561 aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg aggctcgact ccccgccatt
     1621 ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg tacaattggt ggcggaatgg
     1681 ttgagcggaa ggtgattgaa gagtcacttc aggcattgca ggaacgtaag ccgcgattat
     1741 tccatggacg tatggctcgt aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcaa
     1801 tgtcagtatt tatcagcgtc catggtatgc gtccacgtct ggtgttgatt ggcgcggggc
     1861 atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg atttgatatt gccgttgccg
     1921 atatttatcg cgaaagcctc aatcctgaac tattccctcc atcaaccacg cttctccatg
     1981 ctgagtcgtt tggtgcagca gtggaagcac tggatattcg ccctgataat tttgtcctga
     2041 ttgccacgaa taatcaggat cgtgaagccc tcgacaaact cattgaacag cccattgcat
     2101 ggttggggtt gctggcaagt cgccgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga
     2161 aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc cgttggttac aacataggtg
     2221 cggaaacgcc gcaggagatc gccatcagcg tgctggcaga aatattacag gtgaaaaata
     2281 acgcgccggg tgggctgatg atgaaacctt ctcatccttc cggacaccag ctggtggtga
     2341 ttcgcggtgc gggagatatc gccagtggtg tggcgctacg tctgtatcat gcgggtttta
     2401 aagtgatcat gctggaagtg gaaaaaccga cagtgattcg ttgtaccgtg gcgtttgccc
     2461 aggccgtgtt cgatggcgaa atgacggtcg aaggcgttac tgctcgcctg gcaaccagta
     2521 ctgcggaagc gatgaaactt accgaacgcg gattcatccc tgtgatggca gatcccgcct
     2581 gttcattgct tgatgaactg aaaccgcttt gcgtggtgga cgctattctg gcgaaacaga
     2641 atttgggaac gcgggcagat atggcaccag taacaatcgc gcttgggccg ggctttactg
     2701 cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga
     2761 tttactctgg ttgtgcgcag gagaataccg gtgttcctgg caatattatg gggcatacca
     2821 cccgacgggt gatccgtgct cctgctgcag gcattatgcg atccaacgtg aaattaggcg
     2881 atctggtgaa agagggcgat gtgattgcct ggattggtga gcatgaaatt aaagcaccgt
     2941 tgacggggat ggtgcgtggc ttgttgaacg acggcctggc agtggtcggt ggttttaaaa
     3001 ttggtgatat cgatcctcgt ggtgaaacgg ctgatttcac cagcgtttct gataaagccc
     3061 gggcgattgg cggcggcgta cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag
     3121 cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc cggatggtat tacccacccg
     3181 gccttaaaat tactgacaaa tcaccgttcc ggttttgcct tcaatcccct ctttcgcttt
     3241 gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc
     3301 ttccacttta ggcagcatag aacctttcgc aaaatgacct tcttcgataa agcgttcagc
     3361 atcacttaat gacaggcgat cgagccactg ttcattctct ttaccgaagt taatggccac
     3421 tttttctacc gcagtcagaa tgatcagcat atcggcatca atcatttctg ctaaacgggc
     3481 gctggcccag tctttatcga taaccgcgct ggcaccacgc agatggttac cttcacgaat
     3541 aacaggaata ccaccaccgc caacggtgat caccacctgg cctgcatcta ccagagcttt
     3601 aaccgtttct ttttcaataa tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc
     3661 gcgaccagca tcttctttca gagtgtaacc ctgttttgtc agttgctctg cttcttgctc
     3721 ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac
     3781 ttcgacctga gtaaccagcg ttgctaccgg tttattaatg ccccgagaaa gcagttcttc
     3841 tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg cttaaggcaa cgcagacaga
     3901 catcggcagc attggcgagt gcgcttcagt ttttgcagca gcttcaaagg cctggttaat
     3961 catgcccacc tgcggcccgt taccatgagt gacgataact tcatgtcctt gggcaattaa
     4021 atcaacaatt gcctgagaag taatttttac cgctttcatt tgtccggcca ggtcatcgcc
     4081 cagcgcattc ccgcccaggg cgagaacaat tttcttactc atttttcctc actcatcatt
     4141 cttgattgtg tatagatccc tgacagtggt tagagcacgg gagggacaaa cggtttgcgt
     4201 cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca
     4261 ccacgggata aggttctgac aatcgcgccc tgacaggtaa aaccctccca tggcgagtag
     4321 tcggcgttgt cgtggagatg gcgatgctga atttgttggc tctgacgtgg gtcgataatc
     4381 accacgtcgc catcggaacc gggcgctaat aatccttttt gcggccacag gccaaacagc
     4441 ctggcgggca ttgcgctggt taattcaaca aagcgttccg gtgttatacg tcccgtcatc
     4501 acgccactgg aaaacagtaa ctgcatgcgg ttttccacac cgggtaagcc atttgggcag
     4561 cgactgaaat cgcctttaga aatttgcagg cgttgagcca tcgagaaggt gcagtgatcg
     4621 gtcgccacca cgtcaatcgc accatcgctg atgccacacc acagtttgtc ctgctcgcgt
     4681 acgttacgca gcggtgggct aagaatgaac ttcatgccgt cttctgtatc gtaactgcgt
     4741 tcgtccaaca ggagatattg tgggcaggtt tcaacccaga ctggctggtg attcgcacgg
     4801 gcaagacgca gataatccag acctaagccg ttagacaggt gcacgatata gagcggggcg
     4861 ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg cttccgcttc gcattccaga
     4921 gggcgactca aggcatgata gcgcggcgcg gttaaccctg cggcgataaa ttccgcccgc
     4981 ttgctggcga tagccgcatc attttccggg tgcacggtgg tcagcgcgcc ggattcatgc
     5041 agacggcgta atgcctgcaa aacctcgtca tcgttgagtt tgtattgata ggttaaatag
     5101 agtttaaaac tgctcagtcc ttcctcgacc atcatcggga tttcgtcgag gattgcgtga
     5161 ttaatgtgct ggatcacacc gtgaaagctg taatcgatga ccgctttatg ggcggcataa
     5221 ccacgataaa cctccagttg atggcgtaac cgacagccgt ttgggccaaa tcccatatgg
     5281 tcaataatgg ttgttgtacc gccacacgca gctgcgcggg taccggtaaa aaaatcatca
     5341 caactgcgcg cgatgccgac atcaatattg aaatgcgtat ggacatccac gccgccaggg
     5401 aaaacgtaac agccagtggc atcaatttct tcatacggga gctgcggcga aatattgttg
     5461 cccaactggc gaacaatccc gctttcaatc agcaaatcct gtttggcttg tccatctgcg
     5521 ttaacgacag tgccgttttt gatcaatacg cgcatagcaa actccaaaaa ccctccggcg
     5581 aaccggaggg gaataagtga agagtaatta ctctgttgcc agccagctta acgggattgc
     5641 agcgtacatc gcggcacagg tcaccaggtg agatttccag gttttttcgt taggtgcatg
     5701 cgcttcaggt tctttacccg ggccaaagcc gatcaccgga atgccgtgac ggcccatgat
     5761 agatacgccg ttagttgaga aggtccactt atcaacaacc ggcgctttgc caaacagccc
     5821 ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct tcttccactt tccaggtcgg
     5881 gaagtagcat tcggttgggt aaaccaggcc agtccaggac ggacggtcgt agttgtacat
     5941 agaaacaacc gcgttagctt tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc
     6001 ttcccaggtt tcgccccagg tcagacggcg gtcaatagag acggcgcagc tatctgctac
     6061 agcgcaacgg cttggggatg tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag
     6121 gaattcgtca taacccagac gttgggagag ttcttgtaat tcgccaagaa tcggacccat
     6181 tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg tggcagctaa caccctgaac
     6241 atcaatacga atttccatac gaccgcgctg accacggtat atctggcagt cggttggttc
     6301 ggtactgacc acaaattccg ggcgaatgcc ggattgttca ataatgtact gccagcacag
     6361 accgtcgcag tcttcttcct gcacagtacc ggtaaccagc agggtatatt catcttccag
     6421 accgaggtct ttaatgattt taccggcata aaccatagat gccatgccgc cttcctggtc
     6481 ggaagtaccg cgaccaccga ttagctcatc agtttccatg ccttcgtacg gatcgaagtc
     6541 ccagtttttg atgttgccaa tgccgacggt atcgatatga gcgtccattg ccaccagacg
     6601 cgggccgtgg ccgatataac cgagaacgtt gcccatcggg tcgatttcaa ctttatcgaa
     6661 gccgactttt tccatctctt ctttaatacg atgtactacg cgtttttcgt cgcagctttc
     6721 actgggaata gcaaccatgt cgcgcaggaa gcgagtcatg tccgcctggt aatcttttgc
     6781 tttttcaaga atcagtttga atggaatatt cttagccatt atttttctcc agatttttta
     6841 ctccccgttt tgcagggagt agttccaata gagtgattaa ggtgctacag cgtgtttgcc
     6901 ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg ccttcagtgc tgataaccag
     6961 caccacggca tctttgttca gcgccagttt ttccatcagg ctttgacgtt gcgggtgata
     7021 atgaaccgct gcgagaacgc ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg
     7081 gtcgttgccg tacggattac ccagcacgcg catacctaat gcggcaacgc tgtcctggca
     7141 ggagatgaat tgggtggcgc agttacgtag gatttcccag cccagcgggt taggttcgcc
     7201 acaggccagg cctgccatga tggtggccat atcaccgcca acgttgacga tgtcgccttt
     7261 gacgccggag cgataaatac agtcagcttt gtcaggttca acaataatgc tgtgcagatt
     7321 ttgcgggcta tagacgtcga ccagataacc cagcacacca ccggccattg ctccgacacc
     7381 ggcttgcagc agaacgtgcg tcggggttac gcccatttca cgcatttgct cgacggcttc
     7441 atctgccagg gttgcgtagc cttgcatgat ccaggttggg attttggtgt aaccttccca
     7501 tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac
     7561 ggtatcgtca tagttcatat ctgtgacgat gcactcggca ccgaggttca gaatggcgtc
     7621 aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc gcattctgtc cgagttgctg
     7681 cgctgcccac gccacaccgc gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt
     7741 ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt
     7801 ttcacacaat aattgagcga tagcgtacgc accgccgagc attttgaagg cgttcagacc
     7861 gaatcgtttt gattcgtctt tgacaagaat tttcttcacc ccaaaaaggt ttgcgagatc
     7921 gtccagcgca caaagcggtg ttgggcgata accagctatt ttctggtgga actggcgcgc
     7981 cagtttggcc tggctttgcg aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc
     8041 ggcgatatca atcttcaatg agaaaacgga catatcctat ccttttggcc tctggaaata
     8101 gagggccgac cagctgatcg gcccgagggg ttatttcacg cgttcttgcg cttctttcag
     8161 cagttgctcg agcagtgcac ctggtttggc gtatttacgg gacaggatca tcgcggcgat
     8221 gatataaggc ttccagctgg cttctttgta ggtagcgata cggtattttt cgaatacgcc
     8281 ttcagtcact tccccttctt tgcaggatac gccgctgata tcagctggca ggcagtgcat
     8341 gtacagggct tcgccatcac gggtcagttc catcatctct tcagtacaat gccagtcttt
     8401 gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct tttaagcctt cgtgatcgtt
     8461 agcgcgcagc aattcggtac gctgttccat cactttgtaa ggtgcccatg acttcggata
     8521 aacgatgtct gcatctttga acgcttcttc catgctggtg acctgacgga agctaccgcc
     8581 agatgcttta gcattgtttt tcgccacttc aaccacatcc gggatcaggt cgtagccttc
     8641 cggatgggcc agggtgacat ccataccgaa gcgagtcatc agaccgatga tgccttgtgg
     8701 tacagagagc ggtttgccat agcttggaga gtaggcccag gtcatggcga tttttttacc
     8761 tttcaggttt tccagtgaac caaagtgttc acgtaaccac gcgaggtcag ccattgactg
     8821 agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc ggacgctgtg gcagtacacc
     8881 ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc
     8941 cagatacata tcgtcgcgaa taccaatagc gtcggcgcag aaggagatca tattggcggt
     9001 ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct tcgtcgagat cttgttgtgc
     9061 aaggccgagc aggttaagcg cggaagcata agagaagcgg gtacgggtgg agttgtcgcg
     9121 gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt cagcacgcag
     9181 tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt tcatctggcg tctgttccca
     9241 cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc gaattgatat ccttaatcag
     9301 ctcattaaca gttttcatca taaagtcccg gtagtggaat gacaggcaaa gtggtttatt
     9361 tcactgtgac ttgccaaagt gattggtgtt ttcacagcta tacctgggac taacaataag
     9421 tgtgccagaa ttatccacgg ggctgcaaaa gactaatttg acggataatg tgtggggttg
     9481 atcacaaatt gatgccagct tccggttgtt tgtaaaatac gaaaacgttt ggcttttttt
     9541 atcgcgttat ttatttgctg ctaagtatca gaaataagat aaagggacag gctatctctc
     9601 ttcagtttga taaactc
//
LOCUS       DAZKGS010000163         9413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKGS010000163 DAZKGS010000000
VERSION     DAZKGS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            142..1206
                     /gene="ulaG"
                     /locus_tag="ACHOQY_003989"
     CDS             142..1206
                     /gene="ulaG"
                     /locus_tag="ACHOQY_003989"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9679993.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHOQY_003990"
     CDS             1314..2069
                     /gene="ulaR"
                     /locus_tag="ACHOQY_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9679994.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHOQY_003991"
     CDS             complement(2066..2815)
                     /gene="yjfP"
                     /locus_tag="ACHOQY_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9679995.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHOQY_003992"
     CDS             2997..3326
                     /gene="bsmA"
                     /locus_tag="ACHOQY_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9679996.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHOQY_003993"
     CDS             3475..3750
                     /gene="yjfN"
                     /locus_tag="ACHOQY_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9679997.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3867..5492)
                     /locus_tag="ACHOQY_003994"
     CDS             complement(3867..5492)
                     /locus_tag="ACHOQY_003994"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9679998.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5576..6739)
                     /locus_tag="ACHOQY_003995"
     CDS             complement(5576..6739)
                     /locus_tag="ACHOQY_003995"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9679999.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6742..7380)
                     /locus_tag="ACHOQY_003996"
     CDS             complement(6742..7380)
                     /locus_tag="ACHOQY_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9680000.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7390..7788)
                     /locus_tag="ACHOQY_003997"
     CDS             complement(7390..7788)
                     /locus_tag="ACHOQY_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9680001.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7806..8465)
                     /locus_tag="ACHOQY_003998"
     CDS             complement(7806..8465)
                     /locus_tag="ACHOQY_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9680002.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8516..9214)
                     /locus_tag="ACHOQY_003999"
     CDS             complement(8516..9214)
                     /locus_tag="ACHOQY_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9680003.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9233..>9413)
                     /locus_tag="ACHOQY_004000"
     CDS             complement(9233..>9413)
                     /locus_tag="ACHOQY_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9680004.1"
                     /translation="NQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLV
                     DNALDLAEITEEYSH"
BASE COUNT         2439 a         2492 c         2251 g         2231 t
ORIGIN      
        1 ctttccacac tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc
       61 cttcctgaaa aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa
      121 ctatcatgaa ggtaaatggc gatgagtaaa gtgaaaagta tcacccgaga atcctggatc
      181 ctgagcactt tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggtc
      241 gcccctggca catttgcgat gtggtggcta ggctgcaccg ggatctggtt gaaatcagaa
      301 ggtggcacca acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac
      361 ccgttaatga aacagggtca ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca
      421 aacctacgca ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta
      481 ctggcgactc acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag
      541 aactgtgctg atgacgtacc attcattgga ccgaaaacct gcgttgattt gtggattggc
      601 tggggcgtac cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtaaaa
      661 gacattgaaa ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc
      721 gatcaaaaag cggctggcgt actgccggac ggcatggacg atcgcgcggt gaactatctg
      781 ttcaaaacgc ctggcggctc cctgtatcac agcggcgact cccactactc taactattat
      841 gcgaagcacg gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg
      901 cgcggtatta ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat
      961 gcgaaagtgg ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa
     1021 gagatccgcg tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt
     1081 atctggcagg ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac
     1141 tatccgcgcg gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc
     1201 ctgtaatctg tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt
     1261 tcctggctat ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg
     1321 aagcacaaag acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg
     1381 agaaagtcgt tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac
     1441 ttgacgaaag cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccagcagc
     1501 gcccgcgctg gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta
     1561 tcgctaaagc agcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct
     1621 ccaccgcgtt tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt
     1681 atctaccgct ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggtg
     1741 gacagtacaa caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct
     1801 atgccgggca ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa
     1861 ccgatatgct gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg
     1921 tactggttga tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc
     1981 aaatcgatat gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag
     2041 cgcaaggggt cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat
     2101 ctaacgcctc aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt
     2161 tatcccgtcc cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa
     2221 cgtcatcgtc gaggccatgc cacagcagta gaggtctgtc cccaagttgt tccaggtggt
     2281 ttgtcgcttc ccactctgcc agcggcgcga taatgttatt gaattcattc tgctgtgctg
     2341 ccgtttcagg tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc
     2401 ccatcatgct ggcggtacat ttcaccgtgg ggtggcgagt ggtaattccc agtgccgtca
     2461 tcgcgcccat cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg
     2521 ctattgccgc acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt
     2581 gatttaaccg ccgcgcttcg tcgctactaa aacggctacc gtgatcgggc gcatccggca
     2641 tgatcacccg caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg
     2701 atgaagtaaa gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag
     2761 gataagcatg aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc
     2821 tctccttttt gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat
     2881 tagttcataa tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt
     2941 accaggtaac aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg
     3001 ttagcaggaa acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt
     3061 taagtgcctg tagcgctctg caaggtacgc cacagccagc accgccagta acgaaccatc
     3121 cgcaagagat tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga
     3181 tggttcgggg ttctccggat gacgcattag cagaaatcag agcaaaagct gtcgctgcaa
     3241 aagctgatta ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt
     3301 cacaagccat tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct
     3361 ttgcgtccct atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta
     3421 ttaccgcgat ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa
     3481 caattacttg cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag
     3541 tctgcagaat tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca
     3601 gttagcaata tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat
     3661 gaacaaggtg cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac
     3721 tggcgcgtac aggcgatact gtatgcatga gtgttatata cagcaaatga acaatgccgg
     3781 atgcggcgcg agcgccttgt ccggcctaca atgttcgcat atttcattga gttaatcgaa
     3841 accagcgtcg catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat
     3901 aaatcattct ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga
     3961 caccacgcct gcgccattgg cggagaggca tatttcaaca tttgcgcacc gcagcccagc
     4021 aggaatagct gatgagtaat ctctcgcccc agttcttcag ctggcttgcg cagctgctgc
     4081 tgtaaacgac gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaacgct
     4141 tccgacaata agtcgtatac gcccgcttgc ttattgagaa cgcgcagcac atccaggcac
     4201 ataatattgc cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc
     4261 agctcgctct cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac
     4321 ggcatacctc ttttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg
     4381 gcttctttgg catcggcacg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt
     4441 tgcccttcaa gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc
     4501 aatggattac caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg
     4561 cgcatcatgg catggctacc cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg
     4621 atcagacgaa ttccttcccc ttcctgcccc aacaaccagc caatggcatc ctgaaactcc
     4681 acttcgcagc tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc
     4741 gcgttgcgtt gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacaa accgcccgta
     4801 gtctgcgcca gcaccagatg cgcatcgctt tgcggcaccg agaaaaacca tttatgcccc
     4861 accagccgat aagagccatc ttccaggcgc tctgcgcggg tggtgttgct catgacatcg
     4921 gaaccgccct gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct
     4981 ggcaataagt gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc
     5041 gtgggtaaca tctgtaacaa caatggcgtg gcggcaaagg tcatcgttat tggacataac
     5101 gacccagcct caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg
     5161 gagcgagcgt cttcttccca ggcaagattg tgcacccgat tggtacataa cgcctgcatc
     5221 agcaggtgcc aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag
     5281 cgcaataatt caggcggatt cacattcgcc agccgcccca gttcaagaga ttcagccgtt
     5341 cctaactgct gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt
     5401 accgcttcac agagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt
     5461 tgattaaaaa cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac
     5521 ccttagtatg gtcactgcca tggattcctg acagaaagag attgctaaaa cattcttatc
     5581 cagcaatgta atgcggaacg aaacgtgagg tgtctttggt gatcagcgtg ttatcttcac
     5641 ggatccccat tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat
     5701 cgccaaatcg cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac
     5761 catcagcgtg gtcaacgacg ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc
     5821 gcgagtagat cggtttacgc acatagcttt cgccagcggc aatctgcggt ttttcgccct
     5881 caaaccagga tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta
     5941 gccctttatt actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc
     6001 gcagcagcgg accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac
     6061 gctggatgac attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt
     6121 caatgtagat aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca
     6181 cggtagtcct gtcttcatcg gtgtcctgac agcagcagaa ataaaacggc tcccggctat
     6241 aaagctcgct aaagcgtgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac
     6301 gcggaataat gccgctgcgc cgggcatcct ccagccacaa ccactggaaa taagccgact
     6361 catacaacga ggtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac
     6421 accaggcaaa atccatccgg ccatacagcg aaggatcgcg tgcgcgccag ctttcggcga
     6481 tcacatccca gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg
     6541 cgcgatcaac cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct
     6601 cttcaatctg gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt
     6661 cgatgatatg aaagtcgaaa ccgttgttag cggcgatctg gtccagatcc cgtcgcacag
     6721 gaacgttgtg tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc
     6781 atagccgccg cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga
     6841 ctcttttttg ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg
     6901 gcgggaagca aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg
     6961 aataacccgg cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc
     7021 gtagtagcaa ttttcgtact tagactgaca attctgctga gtcatattct tcggaacatc
     7081 ggcataaaat gccgctttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc
     7141 gtcatcaata caatcctgca ctgtcgaata aaacgttccg tcgccgtcgt tatcgacatc
     7201 gccactatcg ccacaacctt tcaatacaaa aaaagcggca ccgcccatta gagccaacgt
     7261 taagtatttt tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat
     7321 gcgactgatc gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat
     7381 gatcgtccat taccatgtca tacaagctgc gttaaaaata ccgccagcca gcgcggcggt
     7441 tcccataaac attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat
     7501 ataaagtctg acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc
     7561 gaaatagtct ggaatactca ccgcattgat tgccgcactg gataatggga taacgtaacc
     7621 taacaatgta ccactgaacg ccagtgaagc cgcggtatta ttgtttttga ttaactgcca
     7681 ttcgttgtgc ggtgtaattt tagaatagat aaaaagaaaa ataatcacca tagccacgcc
     7741 aataaaaaaa taggcgctaa aggcaagaag tgaatccagt atatgcattg taaacgtcct
     7801 tgtaattaac caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa
     7861 aataaccact ctggctcgcc gagatcgtta aaagattcct caccatttaa cagcaaatat
     7921 tcgtaagtat cttcggttac ttgccgctgg tagcccatgg taaaattatg gacctcccat
     7981 ttggcatggt tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt
     8041 tcttcgctgt taaaaaatcg ctgccagcgt ttttcctgcc agtttaagat cattgccccc
     8101 atcactttgg cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct
     8161 tcatagacaa agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg
     8221 atttgcagaa attcatcgcc tgaagtgtag tagcggaaaa tctgactacc gccgcccaga
     8281 tcgatgtggc tgacagcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca
     8341 tcttccagca aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc
     8401 agcggaccgc gagcgatagc aggcttatta tccttgccaa acagacgctg gaaaaaacca
     8461 gacatatttt atccttaaaa tagttgccag ccttttagcg aggctggcaa aaaaattact
     8521 cgccctgttg gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc
     8581 caattccggc ttccgccagc ttttcatcaa gatcgcgacc gtctgcgact ttttccaact
     8641 gtgcagcagc atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg
     8701 attctgccgc tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt
     8761 gtgcacgctg catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga
     8821 tacgctgcgc tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg
     8881 acaacacttg ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt
     8941 cgttaatcaa cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac
     9001 tggctttgcg ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca
     9061 gatcaacgcg agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt
     9121 ccagcatgcg cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt
     9181 tccccagcgt aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg
     9241 aatattcttc tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa
     9301 gcaggatatc ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt
     9361 cctgttgcac gctggagatt cctaccgatg acaacggcat catcttctga ttt
//
LOCUS       DAZKGS010000164         9273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKGS010000164 DAZKGS010000000
VERSION     DAZKGS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..558
                     /locus_tag="ACHOQY_004001"
     CDS             <1..558
                     /locus_tag="ACHOQY_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680005.1"
                     /translation="YHEMYHALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAAC
                     DLVPTQGNDPHHVRLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIF
                     EGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTGY
                     AERYQAIASQYSSRIGVDYVSMMLP"
     gene            complement(630..1046)
                     /locus_tag="ACHOQY_004002"
     CDS             complement(630..1046)
                     /locus_tag="ACHOQY_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680006.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(1085..2314)
                     /locus_tag="ACHOQY_004003"
     CDS             complement(1085..2314)
                     /locus_tag="ACHOQY_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9680007.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(2613..3521)
                     /locus_tag="ACHOQY_004004"
     CDS             complement(2613..3521)
                     /locus_tag="ACHOQY_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFP9680008.1"
                     /translation="MARTLVNVSATIFALMMIVRALFTYIYPGKLPFSIAIIDWLIVI
                     AGSGAAISSIFCFIKKRYPDTAEFLPMFSTVCYVIILIGYAILRYTPAYQTSLSIMVT
                     GMLVGMGWWIQCITSAANTRRSHTLNMIINTRTSPEYQKQLRNSTKFYRGMRYVPQEL
                     SEWRCNPDKEEYKNMKVPDEYRDAINGLLYILNYFEFLAQGIKFKDLDDELLRECFSS
                     FLRGIERRGFHMILESQKQDPAAFEGIIYLSKKWNGTSFVETHRSNPNTVELGVPYPS
                     NDMVEKMVQGQPLIDSDAGPELQVAT"
     gene            complement(4004..5179)
                     /locus_tag="ACHOQY_004005"
     CDS             complement(4004..5179)
                     /locus_tag="ACHOQY_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFP9680009.1"
                     /translation="MGKKEQGLLSLPRGVTIRKHKTASTLVITFTYKGVLCREPLSRL
                     EANTRGIKYAERLLGEIQNQIASGTFEYAKYFPNSKKLELFGVVKKTKNIKSYLDEYL
                     KICINRNLSPSTIGGYEKCLSALSELHKLHVTELTPAILKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVIDSSPSADDYEVDPFTPAETLAIYQSCR
                     YREWENLFRFAFNTGLRSSELCALRWADLDTIANTAHVQAASVVGVLKGTKTKAGTRK
                     VELNSEALAALQAQKQYTFMKSEFIFSDPKTGEPWTNADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRNYGKYLAEYDGKTSINVVSS"
     gene            complement(5134..5343)
                     /locus_tag="ACHOQY_004006"
     CDS             complement(5134..5343)
                     /locus_tag="ACHOQY_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9680010.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLIEVSKWARKNKDYYLCQEE"
     gene            complement(5352..5498)
                     /locus_tag="ACHOQY_004007"
     CDS             complement(5352..5498)
                     /locus_tag="ACHOQY_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680011.1"
                     /translation="MHTVFELWVRKTFGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LNVV"
     gene            complement(5502..5744)
                     /locus_tag="ACHOQY_004008"
     CDS             complement(5502..5744)
                     /locus_tag="ACHOQY_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680012.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRCTDRSVFFRRMDGHSGRVCIG
                     EFNCLFEHIDHQQYCKILADTEQEKHLKKLRAMQRR"
     gene            complement(5829..5993)
                     /locus_tag="ACHOQY_004009"
     CDS             complement(5829..5993)
                     /locus_tag="ACHOQY_004009"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9680013.1"
                     /translation="MPNDAQWCVVNTEYGYYVQCWSEGQGWLGDDISIPECDVTHWMP
                     LPEPPQEVSQ"
     gene            complement(6081..6344)
                     /locus_tag="ACHOQY_004010"
                     /pseudo
     CDS             complement(6081..6344)
                     /locus_tag="ACHOQY_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(6567..6692)
                     /locus_tag="ACHOQY_004011"
                     /pseudo
     CDS             complement(6567..6692)
                     /locus_tag="ACHOQY_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(6694..7023)
                     /locus_tag="ACHOQY_004012"
     CDS             complement(6694..7023)
                     /locus_tag="ACHOQY_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9680014.1"
                     /translation="MSKIDYQALREAAEAIKVVATPQKLLAFRMKVTPQVVLVLLDEL
                     EAKNKRITELEAREVQLPTRYDLRYGHPINADKRHVMIPKENGSWLCLIDLEHALRVA
                     GIRIKGE"
     gene            complement(7020..7259)
                     /locus_tag="ACHOQY_004013"
     CDS             complement(7020..7259)
                     /locus_tag="ACHOQY_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680015.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTREAFFSRIAAQIRKGGNQ"
     gene            complement(7252..7455)
                     /locus_tag="ACHOQY_004014"
     CDS             complement(7252..7455)
                     /locus_tag="ACHOQY_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680016.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(7452..8330)
                     /locus_tag="ACHOQY_004015"
     CDS             complement(7452..8330)
                     /locus_tag="ACHOQY_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFP9680017.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVLKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWQKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(8321..8857)
                     /gene="yfdR"
                     /locus_tag="ACHOQY_004016"
     CDS             complement(8321..8857)
                     /gene="yfdR"
                     /locus_tag="ACHOQY_004016"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9680018.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(8986..>9273)
                     /locus_tag="ACHOQY_004017"
     CDS             complement(8986..>9273)
                     /locus_tag="ACHOQY_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFP9680019.1"
                     /translation="DNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLR
                     LSIITGDRPVLVLRIIQLEAMQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         2366 a         2159 c         2000 g         2748 t
ORIGIN      
        1 tatcacgaaa tgtatcatgc tttaaatatg aatttgtctt caaagaattt tgaagatggt
       61 catatttcta ctggcactat tttctttgat acagagtcga acaaatggta tttatgtgta
      121 tctgcggcat gtgatttggt tcctactcag ggtaacgacc ctcaccatgt aagattaagt
      181 ccgcacaggc tcattaaagt tctggagctt tttaacgcca gtcagagtaa agcattgcca
      241 tttgctgaac attcgaaata tatatatgta atgcataaaa atcaaagaaa atatctctct
      301 attttcgaag gggataaaac gcttcctgtt gttgattata tggtggtgtt gaatcatgga
      361 acaacagttg atggcgaaga aaaaaatatt atttctgccg tgtttttaag taatatggat
      421 ggcaacgtgc aaaatgttcc tgtccgactc aaacttaaat ctcaactgag aactggttat
      481 gcagaaagat atcaggctat agcgtctcag tatagctcaa ggattggtgt ggattatgta
      541 tcaatgatgc taccataatt attatatttt aggcgtggtg attttttttc gccatgccta
      601 ttttatattt atcatcacaa tgatgttgtt tatttttgtt taagtcttct aagcttcatg
      661 cattctaatg agaataataa tagagttgtg ctgtatataa agccagagtt agttaataac
      721 tttaaattta atgcttcagg attttcactg agaaagtgaa aggcataaac taagtataca
      781 gctaaagtag ccgttccaat tgttggtcca atatcgcctt gatcatgagg ttcaaggttt
      841 acattgaact tgaagattag ccattcaaat ccccaagtaa aaaaaacaac taatatcatg
      901 gctgcgaata atttcgcgat gttatctgct gatggattca tcagaacaga ttgtttctga
      961 aaaaattcac aaagtgagag tccgaaaaaa ataaaaaaaa gtggtctgga actaaatttt
     1021 tcaaaaactt tgaatagggt ttccattttc tttaccttca ggattatatt agctattctg
     1081 atacttaggc taccagagca tttgatcttt gagcttgaac gtaatcactc caccattgca
     1141 tcaaactctg tcgctctatc agatattctg cacgattgta tgctgcgata atttcatctt
     1201 ttttcgagtg ggcaagcgct gcctcaagaa cttcagctct gaatttacca gactcctctg
     1261 ccgctgttcg tgcaatagaa cgcataccgt gagctacaag ctcgcctccg aaccccattc
     1321 ggatgatagc tgcgttggct gtttgttcat gcatatgatt aagaggcgct tttatgctgg
     1381 ggaaaaccca ttctctatgc ccacttattg atttcattaa ttcaaggatg cgcaaagctt
     1441 ctttactcaa aggaactttg tgaagctttt tcattttcat gaaatcagca ggaatgttcc
     1501 aaatgctgtt ggttgtatca atatcagacc accttgcgcg aacggcttca cccggacgaa
     1561 cccatgtcaa caattgccat tcaattagca tacgtgtttc caaccggatt gacgcattcg
     1621 tcaaagattc cataaacctt ggcaattcgc ttgggggaag ggcaggcata ttttgctttt
     1681 ttggtttact gaatctttga ccaaggttgt cagccgggtt gaactcaata agttcttcag
     1741 tagctgccca ccggaagatt tcattcagac gtgaaatgat acggcgtaga gtttccaata
     1801 cgcctcgttg ctcaatagga tcaaggtgtt gttttaagag cttaggtcgg atctcattga
     1861 tagggacatt acccagaccg ggaaagacat ttctctctaa gctgcgccag atgtctgctg
     1921 catggtcttg tgagatacct gatgtcttta ccttctcatc taaccatttc cgcgctacgg
     1981 cttggagagt gtgctcagta gcactcttta atgccttcgc cttatcgttg ttatggattt
     2041 ggggatcaac accatttgcc agaaaggaga gatattcatc acgtaaggct ctggctcttg
     2101 caagggtaag gtgaggatat gtcccaaggc tcattttggt tcttttcttg ctcacgggta
     2161 ctgcatacct gaaataccaa tttttcttgc ctcctttcgc caaaggagcg attcgtagaa
     2221 tcagaccatc accgtcaaac aagttgattt ctttatcggc tggcttggtg cttttgattt
     2281 cagtgtcagt gagcttctta gcgatttttg ccattttggg accctcggtt tttggaccct
     2341 tcttagtggg tcccattcag ggtgccataa ctcgtagttc tcagcaattc tcactggacg
     2401 acaatagacg taaaaaagcc cgcagagctt gtgctgtgtg ggcttagtag acttcattga
     2461 acttcaaaca actaaaaagt ggagctggcg ggagttgaac ccgtgcccga gcaatgctta
     2521 acccattgaa taacaatgca ttctctgttt aaaaatgcct caagtgcatt ttgagtgcat
     2581 tttctggtcc tctcttggtc catacaacga gattaagtgg ccacttgaag ttccggccct
     2641 gcgtcgctat ctattaacgg ctgaccttga accatttttt caaccatatc gtttgaagga
     2701 tatggaaccc cgagctcaac cgtattaggg ttggaacggt gggtttcaac aaacgatgtt
     2761 ccgttccatt tttttgaaag gtagattatt ccttcaaagg ctgcagggtc ctgtttctga
     2821 gactctaaga tcatgtggaa gccacgacgt tctatcccgc gtaagaagct ggagaagcac
     2881 tccctcagta attcgtcatc caaatccttg aacttgatac cctgcgcaag aaactcgaaa
     2941 taattaagaa tatataaaag gccattgatg gcatccctgt actcatcggg gaccttcata
     3001 ttcttgtatt cttctttgtc tgggttgcag cgccactctg atagttcttg tggtacataa
     3061 cgcatgcccc gatagaattt ggtgctgttc cgaagctgtt tttggtactc cgggcttgtt
     3121 cgggtgttaa tgatcatgtt cagcgtgtgg gacctgcgcg tgttagcagc ggacgttatg
     3181 cattgtatcc accatcccat tccaacgagc attcctgtaa ccatgatcga aagcgatgtt
     3241 tgataggccg gtgtgtacct taaaattgca tatccaataa ggataatcac ataacaaaca
     3301 gtgctgaaca taggaagaaa ttctgcagtg tctggatagc gttttttgat gaagcagaag
     3361 atagaagata ttgctgcacc agaaccagca atgacgatca gccaatcaat gatggcgatg
     3421 ctaaatggta gttttccagg gtagatataa gtaaaaagtg ctctgacaat catcattaga
     3481 gcaaaaattg tagcgctgac gttgactaat gttctcgcca tcttattgat tcctataaca
     3541 aaagacctcc gcatgggagg ccttattgtg ctaatggagg tttgttttaa aattttaggt
     3601 tgtagttaac cttcaccgaa acctacggtc agtgtctttt tcatagtgtt cactccagtt
     3661 gtggtgcttc gccgatacct acgttaagct cttgaaaagt aaaaaaacat aaaagtttta
     3721 gaatttgtgg agcacaggat aacagtttgt actgggactc atcaagtact atcagtgtcc
     3781 ttgcactgtc ctagtgaaaa tttattgcaa taaagtaact tacgtgaagt caagaaatat
     3841 atttgcactc catttcttgc tatgatgtaa gttattgatt ttaattaaat ttacaattac
     3901 ctaaatgaac tatggaaact atcaataaca tagatgatag ctcatttcac aaaaagcaat
     3961 ggtagactca ttcttaattt tcatcagcct taagcctatg tactcagctt gacacaacat
     4021 ttatagacgt tttaccatcg tattcagcca ggtatttacc gtaattgcgg aacagcattt
     4081 ccggcccctt atgccccatc tgcccggcaa gccagaaaag gtttacaccc tggctaatgt
     4141 gtctggtggc gaatgtgtgg cgcgtctggt acgggttacg gtagcgcaca ccagcttttt
     4201 tcagggtcgg cacccatgct tttttacgga tagcgtcggc gttcgtccag ggttctcccg
     4261 ttttcggatc gctgaatatg aactcacttt tcataaaggt gtattgcttc tgcgcctgca
     4321 gggctgccag cgcctcactg ttcagctcca ccttacgggt accggctttt gtcttggtgc
     4381 ctttaagtac ccctacgaca ctggccgcct gaacgtgggc tgtgttcgcg atggtgtcga
     4441 gatcagccca gcgcagcgcg cacagttctg agctccgcag acctgtattg aaagcaaagc
     4501 ggaacaggtt ttcccattcc ctgtacctgc agctctggta aatggcaagg gtctccgctg
     4561 gcgtgaacgg gtcaacctcg taatcgtcgg cgctcgggct actgtcgatc acgtggtacc
     4621 ggctggcgct gacgagggtt accgggttaa tggtcagcag gccatccgta acagcttcat
     4681 caatggcgct gcgcagaaat gacaggttat tcctgattgt tttcagctta gtttttcggc
     4741 tggctatcca attttttagt atcgcaggcg tcaattctgt tacatgtagt ttatgtagtt
     4801 ctgatagtgc tgatagacat ttttcgtaac cgccaatggt tgacggcgac agattacggt
     4861 ttatgcaaat tttcaggtat tcatcaagat aggacttaat atttttggtt ttctttacta
     4921 caccgaataa ctccagtttt ttggaattgg ggaaatattt cgcatattca aacgttccgc
     4981 tggcgatctg attttgtatc tcccctagca ggcgctcagc atattttata ccacgtgtat
     5041 ttgcctcaag ccgagatagg ggctccctgc aaagaacccc tttatatgtg aaagtgataa
     5101 ctagtgttga agcagtttta tgcttacgaa tagttactcc tcttggcaga gataataatc
     5161 cttgttcttt cttgcccatt ttgaaacctc tattaagtcg acccaacgct ctttaacccc
     5221 gtcgactttt aatacatgta ccccttcttt ccatattcct ctttgtatcc gtttgttaac
     5281 ggcatcaacc gtttctcccg cgttgcggca gtaggttgat ataggtacgc aatccagccc
     5341 cacagttcac ctcacacaac attcagccca cgacagtgac accacacttc aaacattcgt
     5401 ttcacaattt cacgacagta gaaaccgtca acatctcgcg tcaggtcata gcgattgccg
     5461 aatgtcttgc gaacccataa ctcaaaaacc gtatgcatgt atcacctccg ttgcattgcg
     5521 cgcaattttt tcaggtgctt ttcctgctca gtgtccgcca ggattttgca atactgctgg
     5581 tggtcaatat gttcgaacag gcagttgaac tcaccaatgc atacccgccc ggagtgtccg
     5641 tccatacgtc gaaagaacac agagcggtcc gtacagcgag taatcaccac agggtatccg
     5701 gccctgtccg tgtatatttg cccgcgttga ataagtgcga acatgagatt atccccagcg
     5761 gcaaatcgaa aaaataatca gcgcaatcga tattgcaaca cctacaattg caaatgcttc
     5821 aggccaactc attgactcac ctcctgcggc ggttctggta gaggcatcca gtgggttaca
     5881 tcgcattcag ggatgctgat atcatcacca agccaccctt gaccttcaga ccagcattgc
     5941 acgtaatacc cgtattctgt gttcactacg caccactgtg cgtcgttcgg cattagctca
     6001 ctacagctta tccaaccatc cggagttacc ggaacttgcg gaatggctgt ctgctctcga
     6061 acgtcattag gcgctatagg ttctgctgcc aactgactgg catatttgtt aatggtaacg
     6121 ataagctctt gctcagcctc atccagataa tcaccgatac ctcgcctgtc accgtcaaaa
     6181 tcatcgaaat cggcacgaat cttggcaacc ttctggattg cggacaacac ctcactagga
     6241 attaccggat agttggttga cgtttccgcg atttcccgaa aattattggt tgacgaattc
     6301 ttattttccc gaaagtttct ggcctgaagc atggcggcgc ggcaggcatt ccagccagta
     6361 gcgtaatatt tcgcatcctg ccagctcgtt tgtacatttg agtacgcttt ttcctctgtc
     6421 atggcctctg gtattgatgc cggtgcaatt cgtggcaggc cgatatatag tggtacatct
     6481 cccggctcca tcgaattgac gggccaaata tacgcatcac taccatggtt aatgagcgca
     6541 atccccgaag tatcgatgat gccaaatggc tccgctgtca atgcagtcaa tgcaatttca
     6601 taagcccgac gctcaatatt gtctcgcacg tccaggctgc ttatgcgctc tttgatttct
     6661 ttaatcagtt tcttatcggt gaaagtggtc atctcactct cctttgatgc gaatgccagc
     6721 gacgcgtagt gcgtgttcta agtcaatcag gcaaagccag ctgccatttt ctttaggtat
     6781 catgacatgt cgcttatcag catttattgg gtgcccatat cgaaggtcgt agcgagtcgg
     6841 taattgaact tccctcgctt ccagttctgt aatgcgtttg tttttagctt ctagttcatc
     6901 cagcagcacc agcacaacct gcggtgtgac tttcatacga aacgcaagca atttttgcgg
     6961 cgttgccacg acttttattg cttctgccgc ctcacgcagt gcctgatagt caatcttgct
     7021 cactggttgc ctcctttacg gatctgcgct gcgatgcgcg aaaaaaaagc ctcccgcgta
     7081 tggctgttaa gagctggcgc gaacgccgcg ttaagaacgg cagcatcaca gccgtcatcg
     7141 atatagagcg caattttttt ctccaggcgc gctttggctt cctgcaactg cataccccgg
     7201 cacgcacgcg ggatataatc agcaatttga gcgatagctt tttcgttctg tttaaacatg
     7261 cttcacctcg ataggcttga tggtgtctaa cagcagtcgg cggcgcgtat tttctgcaaa
     7321 atggcggcgt ccagtttctt tgtggtaaaa ctcgtttttg ccaacgaccc acatccgctc
     7381 tgtttggtgc aattttttta cctgcgggcc atctttggtg atcacggtac cggtatgggt
     7441 ttttacgatt gtcatgccac gacctcttcg aatttcaact ccaattggtc accccagatt
     7501 tcacatgatt cggaacacga accagtatca aaccgcttgg ccagcaccat cgcctgatac
     7561 aaacttctgt agtcgctgtc ggcatacatt ctggcaatcc cgtcaagcgt caggtgacca
     7621 cggtacataa cgtctttacc tgttctgcga tgaccatccc tgacgtgttt gcctgtaacc
     7681 agttcattaa aaacccgcat cagaccaggt tcgtctttac atgcaagccc cagcttttgc
     7741 gttgactttt tgatgcaaaa aacacagttc ccgaggtgct ccgggatttg caaatcaaaa
     7801 ggttgttttt gccaccaccg gataacatcc gacttatcaa aatctgacag ctcggcaaga
     7861 taccggacgc caggtttcgg tttcagccta cggggttcgt ccgcacgaat accgagccat
     7921 gtgatgtaat tccctcgccc aaaatggtca tcgcaatatt tcgtgaaagg ggtgagtttt
     7981 agcctgtcag tacagaatgc gccgccgatg tatggcgtac cgtacttttt aaccatgtcc
     8041 ataaacggtt taagcaccgg cattcgcgtc tgaatatcct ttggttccca ttccgtataa
     8101 ccatttggct gcccaagctc tggatttata tcgacctgca acacagttag cggtatgccc
     8161 cagaacttca aaacctcccg aataaagcgg tatgtcagcg gatgttcgca accggtatcc
     8221 ataaagatgt agcagacgtt attgccagcc tttctttgtt cttccatcag gtgaacaaga
     8281 tatgcagatg ttctcccgcc agaaaaacta actacatgag ttatgcacat ttgcgtaatt
     8341 ccgataactc gttgaagcgt tccataaaca tcccgtaggc atggcccggt gccagtggaa
     8401 tcactttgaa catctctgtt gccgggatac cttccaatac aggccagaaa gagccatcat
     8461 caagtccgag atcgcggcgt tcggttgcca gcatgatgag atcggcatat ttcactggcg
     8521 tgctcataac aggaggtaac ccgtatttct cacggattac ggcgtctatt ttttcttcca
     8581 tccgtttata gtcaggaaga agtcgtttca gtggcgcggg gatgtcctgg caatatgctt
     8641 ctgttgcatc atgcattaac gcttcaaaag caaattcctg cggcaccagc tggctgcaaa
     8701 gcaccgcatg ctgggcgaca ctgtagaagt gtgaaagatg tcctgcaaag cgacagatat
     8761 ttgaaaggga aactgcgata tcgttaatca cgatgtcgtc tttatttatc ttgtcataat
     8821 aaaaatgctt cccggaaaaa gttttaataa atgacatttc gttctccact ttatatgcgc
     8881 tgcaccgcgc tgaattctgc taaaaggaag ctctcaccat ccggtgatta ttgagttaat
     8941 tacgtttcca taaatgcccc cgcaggggca tttgcagtaa tgaaatcagg cggtgaaagt
     9001 accaataaag gtttctactt tgctgtcttt gaatttctca acaagcagat cacgaaattc
     9061 gttagccatt tcttcctgca tcgcttccag ctgaataatg cgcagaacca gtacaggacg
     9121 atcgccagtg ataatgctga ggcgtaattt aaacggacgt tctttcaggc cttcaaacgg
     9181 aacgcattta aattcaaatg ccactggcat aatgtctttg gtcttcgctt cgacagactc
     9241 catcagggag cgtttgccgc tgaagtcatt gtc
//
LOCUS       DAZKGS010000165         9203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKGS010000165 DAZKGS010000000
VERSION     DAZKGS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(356..1456)
                     /gene="chaA"
                     /locus_tag="ACHOQY_004018"
     CDS             complement(356..1456)
                     /gene="chaA"
                     /locus_tag="ACHOQY_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9680020.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1726..1956
                     /gene="chaB"
                     /locus_tag="ACHOQY_004019"
     CDS             1726..1956
                     /gene="chaB"
                     /locus_tag="ACHOQY_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9680021.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2114..2809
                     /gene="chaC"
                     /locus_tag="ACHOQY_004020"
     CDS             2114..2809
                     /gene="chaC"
                     /locus_tag="ACHOQY_004020"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9680022.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2853..3206)
                     /gene="ychN"
                     /locus_tag="ACHOQY_004021"
     CDS             complement(2853..3206)
                     /gene="ychN"
                     /locus_tag="ACHOQY_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9680023.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3391..4785
                     /gene="ychO"
                     /locus_tag="ACHOQY_004022"
                     /gene_synonym="iatA"
     CDS             3391..4785
                     /gene="ychO"
                     /locus_tag="ACHOQY_004022"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9680024.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4786..5436)
                     /gene="narL"
                     /locus_tag="ACHOQY_004023"
     CDS             complement(4786..5436)
                     /gene="narL"
                     /locus_tag="ACHOQY_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9680025.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5429..7225)
                     /gene="narX"
                     /locus_tag="ACHOQY_004024"
     CDS             complement(5429..7225)
                     /gene="narX"
                     /locus_tag="ACHOQY_004024"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9680026.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7564..8955
                     /gene="narK"
                     /locus_tag="ACHOQY_004025"
     CDS             7564..8955
                     /gene="narK"
                     /locus_tag="ACHOQY_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9680027.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2240 a         2240 c         2358 g         2365 t
ORIGIN      
        1 ctggtttcaa gattagcccc cgttttgttg tcaggtttta cctctcaacg tgcgggggtt
       61 ttctctttcc agcaaccaat gccaccaggg ataaagcccc cgcaacattg cgcctcaccg
      121 gataacgccg gcttggtgtg gatactacgt cgcaattcat cttcacttca tccctgaaat
      181 gtttgcaata aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt
      241 tgtggtacag gctcgcaatt ctgattaacg agctgtctag gtagtatgaa gaaatacaat
      301 aagcaggaat ttatcttatc tcccccgatt tccgtcatcg gaggagatat aactgtcagg
      361 caaatatcgt catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt
      421 tagtacgtcc ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca
      481 tttctggcgc accaagtgca aactgtaatt cgttacccgt cataaaggca attagcgtga
      541 cgacaggtac ggtaagcgaa atggttgcta acactgaacc aaagaacaga ttcatcgcgc
      601 gctggacctg gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca
      661 acagagccac caggaagcca gtaaaggcga caggggcatt catgctgtcg agcaatgtct
      721 ccagcgggct ggcattcatt ttggtgactg caataacggc aatcagatgg ataatcaacc
      781 agatagcatg ccacacgctg ctatgggcag acggtttacc gtgatgcggg tcatcgtcat
      841 cactgtcatc ttcgtgctcg tagacaaaca aactttgatg cgttttggtc tggatcagca
      901 aaaatacgcc atacattgcc gcagaaatta atgctaccag taacgcctga ccggttgaaa
      961 aattcgccgc aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg
     1021 caattaaata ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc
     1081 ccaacaataa tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg
     1141 tatcacgcat tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga
     1201 cttcaagaat aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat
     1261 gggctaatac gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg
     1321 caagaagatt gatggcaatg accactggta gtgtctggct gcttccccac aggaacagca
     1381 ctaccagcgc cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct
     1441 cttgagcatt tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa
     1501 taggtaggaa aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc
     1561 cactgtcgat tttttactca ttgggacata aaaataagta ttacgtttag acaatgtttg
     1621 ttttagcgtt tcttaagaac agtctgacat gaaaattctt atgttttggc aagagtagat
     1681 attgttgacc acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg
     1741 aaaagcgatc tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat
     1801 aaagaagcgt tcaacagcgc atgggatcaa tataaagata aagacgatcg gcgtgatgac
     1861 gccagtcgcg aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc
     1921 aaaggggatg atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat
     1981 tcgtgcggtg ttgccttgca agtggtccgt ggattgcata ttgtcccgtt agtggtttca
     2041 aaatgagcag taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg
     2101 gcggaggtgt aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt
     2161 tggtgccatt gaagaatcac tcttatggtc agcagagcaa cgggcggctt cgctggcggc
     2221 gacgctggct tgtcgacctg atgagggacc ggtgtggatc ttcggttatg gatcgttgat
     2281 gtggaatccg gcactggagt ttaccgagtc gtgcaccggt acactggttg gatggcatcg
     2341 cgcgttttgc ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct
     2401 tgcactgaaa gagggcggac gcactacagg cgtcgcctat cgactgccag aagagacgct
     2461 ggagcaggaa ctaaccctgt tgtggaagcg cgagatgatt accggctgtt atctgccaac
     2521 ctggtgtcag cttgatcttg atgatggatg cacagtaaac gccattgtgt ttattatgga
     2581 cccgcgacat ccagaatatg aatctgacac tcgcgctcag gtcatcgcgc cgttgattgc
     2641 ggcggcgagc ggtccgctgg gaaccaacgc acaatacctg ttttcactgg aacaggagct
     2701 tatcaaactg ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact
     2761 gctggcggag aattatccgg atggtgtgtt acggccggga ttcgcctgag taaacttccc
     2821 tcatagtggg gcgtcagacg ccccctcaaa cattaaaatg tgagcacttt atcggctgac
     2881 agcgtccatt gcgccagttc cacaagagta ccgatttcca ccccatcaat cagaggaagt
     2941 gtactaatcc cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg
     3001 gtaaggatct ccagcatttg ctgaatgttg tagccttccc ctggtttttg cccgcgcaac
     3061 ccggctgtga ccgcatcaga catcaggaac agacgcagat ccagattgct ctcctgctct
     3121 cgtaacgcaa tggctagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca
     3181 ttggcaacga tcacgatttt ttgcattatt tactcctgta ttcagggaat tagacattca
     3241 tcttctatct tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc
     3301 tgaaagcgcg agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt
     3361 ttttcattat taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg
     3421 ttttatttac tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa
     3481 aaagcggcaa atccctttga taataacaat gatggtctgc cggatttagg catggctccc
     3541 gaaaatcatg atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt
     3601 atgaatgata acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc
     3661 cgcgacgcgc ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga
     3721 aatgccagtg ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc
     3781 tggtttgtgc cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact
     3841 cagcaggatg atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac
     3901 tggctggtgg gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg
     3961 ggctttggtg ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagccg
     4021 tttgctgcat ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtatgac
     4081 ctgacagccc ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa
     4141 cagtattttg gtgatcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc
     4201 gcgttgagtc tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat
     4261 aaacagggtg aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt
     4321 ggtgtaccgc tcaaaaaaca actttctgcg ggtgaggttg ccgaaagtca gtcgttacgt
     4381 ggtagtcgct atgacaatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga
     4441 aaaacgttaa cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg
     4501 ccgctgaaat tacaaatccg cagtcgttac ggtattcggc aactgatttg gcagggcgat
     4561 acgcagatat taagtttgac gccgggcgca caagccaaca gcgcggaggg ctggacgctg
     4621 atcatgcctg actggcagaa cggggaaggg gcaagcaatc actggcgatt gtcggtggtg
     4681 gtggaagata accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa
     4741 ccgttcgatg cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc
     4801 ctgatgcacc cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg
     4861 cttgacgtgc acttttactg tgctttcggt gatatccagg cggcgggcaa tcatcttgtt
     4921 cggcaaaccc tgggcaatca gcttgagaat atcgcgctcg cgtggggtta actggttaac
     4981 atcgcgctca gtagtggcac ggttagcgcg caagctggcg gccagaacag gcgttaatgc
     5041 ttcgcttaat accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg
     5101 ttccatatct tttaacagat aaccatccgc gccgcgtttc agtgcggtga ccacatcttc
     5161 ttcatggtta gagacgctga ataccacaat gcgccctgag agggactttt cgcgcagttt
     5221 atccagcgtt tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg
     5281 atcaagagac tccgccagtt caataccctg ttcgccatta ctcgcttcgc caaccacggt
     5341 gatatctggt gccatactga taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc
     5401 aatcagcagg atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga
     5461 aagttttttc gggaataaag gtgaccacca cttcggtgcc acctgattca cgacggcgga
     5521 cgcggcaatc gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat
     5581 tgctgcggat ggcattttca ggcacgccgc agccgttatc ctggacggtc agtttgacct
     5641 gattatcgtt ttgcgccacc gtcaccacga cttcactcgc ttgcgagtgt ttgagggcgt
     5701 tacttaatgc ctcacgggca atttgcaaca agtggattgc ctgatgcgaa ggcaccaggc
     5761 gaggcggcaa ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc
     5821 aactcgcctc cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga
     5881 gcaattcacg caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt
     5941 cgcggctgct ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca
     6001 tgcaagagag agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt
     6061 ccatcacgat caactgttgc tgacgttcct gatggcgatc cagcgccagc gtggcggtga
     6121 gttgttcaac cagggtatcc accagttgtt gttgatcatg gctaagatga cgcccctgcg
     6181 gcagggtcgc cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg
     6241 ttgtgccgcg atcaccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat
     6301 cacaagtcat atctggttgg caggtaaact cctgatgatt ctcttcatca tcagtatcat
     6361 aaacccgcag ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtacaggtg
     6421 acaggcgttc acacagcggg gcttgggaat gcaaacggcg gttagcctgc cataaaaaag
     6481 agaggatctg atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta
     6541 cggcataact ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca
     6601 tttcgttgcg cccgctgatg tttgcgcgtt gggtaaaatc gcgatgactg acggcactcg
     6661 ccattgccag cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga
     6721 acaccagtaa aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcga
     6781 tgcgcatttc cgtggtgcgg tcaaaaccag ataccagttg atcaagcccg gcaacaaact
     6841 ggctgacatc cgctgacacc gtttctcggt tttgtgcacg catcaacgca gggatcagtt
     6901 cattacgcca gtaatcttgt aaaccctgta attgcgccag ttgtccgtct cgttctgctg
     6961 ctcgagtcaa ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt
     7021 tctcgcttaa tggcactgct gccaacagac ggtaactttg catgcgcagc gatcccgctt
     7081 tgttgatcgc atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc
     7141 ctgccagtcc aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga
     7201 gcggagagag acaacgttta agcatgtaaa cctcttcctt caggctttaa atgagcaata
     7261 accttaatga atgtgacgat acattctgga atggcagtat tctcggctat tggctgaagt
     7321 atacccatac ctggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgt
     7381 taagggtagg gattctacag caccgtgaaa aatctcataa tttttatgaa atcactgtac
     7441 tcactatggg taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca
     7501 tcaaattgcc tttagctaca gacactaagg tggcagacat cgaaacgagt atcagaggtg
     7561 tctatgagtc actcatccgc ccccgaaagg gctactggag ctgtcattac agattggcga
     7621 ccggaagatc ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg
     7681 atttccgttc cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg
     7741 gtgaacctac cgaaagtcgg ctttaatttt acgaccgatc agctatttat gttgactgcg
     7801 ctgccttcgg tttctggcgc gttattacgt gttccatact cctttatggt tcctatcttc
     7861 ggtggtcgtc gctggacggc gttcagcacc ggtattctga ttattccttg cgtctggctg
     7921 ggttttgccg tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgcta
     7981 tgcggctttg ctggcgcgaa cttcgcctcc agtatggcaa acatcagctt cttctttccg
     8041 aaacagaagc agggtggcgc gctgggtctg aatggtggtc tgggcaacat gggcgtcagc
     8101 gtcatgcagt tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc
     8161 cagggcgtca aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg
     8221 gtgccgttcc ttgccatctt cactattgcg gcgtggtttg gcatgaacga tcttgctacc
     8281 tcgaaagcct ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg
     8341 agcctgctgt atctggcaac cttcggctcc ttcatcggct tctccgcggg ctttgcaatg
     8401 ctgtcaaaaa cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt
     8461 attggtgcgc tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt
     8521 gtcacgctgg tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta
     8581 ccgactgacg ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc
     8641 ctgacagctg ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa
     8701 ctgacaatgg atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg
     8761 gcaaccgaca cggcagcggc gctgggtttc atctctgcga ttggcgctat tggtggcttc
     8821 tttatcccga aagcgtttgg tagctcgctg gcattaacgg gttcgccagt cggcgcaatg
     8881 aaggtatttt tgattttcta tatcgcctgc gtagtgatta cctgggcggt atatggtcgg
     8941 cattctaaaa aataaaccgt tactcgtcat acttcgggtt acatgtgctg cggctgcgtt
     9001 cattcacccc agtcacttac tttagtaagc tcctgggatt cattcacttg ccgccttcct
     9061 gtaaaccgaa ttatatagag taaaatattt gattatcctt tgcgcggcat gatgtcgcgc
     9121 tttttttatg cgttatttag ttacaacata ctaatgttat atggcttatt cgccggattt
     9181 cattaagagc cattaatatg tta
//
LOCUS       DAZKGS010000166         9185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKGS010000166 DAZKGS010000000
VERSION     DAZKGS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..848
                     /locus_tag="ACHOQY_004026"
     CDS             <1..848
                     /locus_tag="ACHOQY_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
                     /protein_id="HFP9680028.1"
                     /translation="PVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKKEIQRVY
                     DENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAG
                     DRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVL
                     DALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESI
                     NGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGN
                     DDLAA"
     gene            complement(891..1230)
                     /locus_tag="ACHOQY_004027"
                     /pseudo
     CDS             complement(891..1230)
                     /locus_tag="ACHOQY_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1546..2190)
                     /locus_tag="ACHOQY_004028"
     CDS             complement(1546..2190)
                     /locus_tag="ACHOQY_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680029.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(2211..2480)
                     /locus_tag="ACHOQY_004029"
     CDS             complement(2211..2480)
                     /locus_tag="ACHOQY_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9680030.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(2559..3197)
                     /locus_tag="ACHOQY_004030"
     CDS             complement(2559..3197)
                     /locus_tag="ACHOQY_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFP9680031.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(3182..4413)
                     /locus_tag="ACHOQY_004031"
                     /pseudo
     CDS             complement(3182..4413)
                     /locus_tag="ACHOQY_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4914..7595)
                     /locus_tag="ACHOQY_004032"
     CDS             complement(4914..7595)
                     /locus_tag="ACHOQY_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP9680032.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(7836..8882)
                     /locus_tag="ACHOQY_004033"
     CDS             complement(7836..8882)
                     /locus_tag="ACHOQY_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9680033.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8986..9135)
                     /locus_tag="ACHOQY_004034"
     CDS             complement(8986..9135)
                     /locus_tag="ACHOQY_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680034.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
BASE COUNT         2461 a         2336 c         1926 g         2462 t
ORIGIN      
        1 gaccggtatg cagcgaactg catattgccc cgtcaacgta ttaccattgt cagcaacagc
       61 gacatcatcc ggataaacgc agtgcccgtg cgcagcacga tgactggctg aagaaagaga
      121 tacagcgcgt atacgatgaa aatcaccagg tatacggtgt gcgtaaagtc tggcgtcagt
      181 tgttacggga aggtatcaga gtggccagat gcactgtggc acgtctcatg gcagttatgg
      241 gacttgccgg tgttctccgg ggtaaaaagg tccgtacgac catcagccgg aaagccgttg
      301 ccgcaggcga ccgcgtaaac cgtcagttcg tggcagaacg acctgaccag ctgtgggtgg
      361 ctgattttac ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg
      421 tgtttgccgg atacattgtg gggtggcggg tctcatcgtc catggagacg acattcgtgc
      481 tggatgcact ggagcaggcg ttatgggccc gtcgaccgtc cggcacggtc catcacagtg
      541 ataaaggttc tcagtatgta tcgctggcct acacacagcg gcttaaggaa gccggattac
      601 tggcatcaac aggaagtaca ggcgactcgt atgacaacgc gatggcggag agcatcaatg
      661 gtctttacaa agcggaggta atacaccgta agagctggaa aaaccgtgca gaagtggaac
      721 tggccacact cacgtgggtg gactggtata acaatcgacg attgctggaa aggctgggcc
      781 atactcctcc ggcagaagca gaaaaagctt attatgcttc catcggaaac gatgatctgg
      841 cagcctgagt tcacagataa aacactctcc aggaaacccg gggcggttca aacctgattc
      901 tggaaaagga ctttgtggat tgttgctatt gccagtgaga ttgaatgaag tcgttttaac
      961 tcgctttgaa tacgtcttgc ccccagattt cgctgagaac gtaactccag aatcagagcg
     1021 acttcgcaag tgccggtttt cgttgatggt gaacgcttgg ggcgacggct gtggctttcc
     1081 agaccagcga tgccctgagc cagatatctg cgccaccatt ttacgcaatg tgggccgaga
     1141 aatgccacag cgacggcata caaatcccgc atcaccggac gtttcataga gttttaccca
     1201 ctgtagccgc tgctgaattt ccctgttcat aatttggcat tagttcagct caatcccgac
     1261 ttaaaaagtc cctacaggat gttccggacc tgaggcacag ttcatatcct tatccataaa
     1321 aaatttagct atgtctatag cattaatctt gacaagacga cgttagggcc tggcgatgaa
     1381 tcatttcccc tgccagatat cctttggggt gcaccagaac taccgtttta tatctatata
     1441 actgaaacgt ccctcgccat ccttcatatt ttgatgtagt aacaccattc actttcatct
     1501 accccccgtt ttattaacat atagtaaaaa tcacctcgcc gcattttatt cttttgaagt
     1561 aaaatcattt accagttcac ttaaagctct cttttgttca tcatcataca gagattgaat
     1621 ccggaattta atctcttctg caaccagtct tgcatcgggc tcataaaata tttttgttat
     1681 tcgctgtttc tgatgttcag gctggtcttc catatttcgt aaaaccagat gaaataatat
     1741 atcataaagt gcgcaatgcc atgaataacc atcattggtt aaaataagct cccaaacccg
     1801 ctcctgctcg gcaataagct gcagaacaaa ggactcaacc tgttctgttt gttcgggggc
     1861 aacacggtat agcggagcaa tcacagcaaa agatagttca tttaaccagg attccgcttc
     1921 cttctcgctc ctgaacggtg gacagttttg tattcttcgt tcgtattcac gggtaatctg
     1981 ctccagaata tcctccggcg gtgtcagcca cttattcctg agcaatacac tggttgatgg
     2041 atgacagctg gctatttcag caataatttc acgtaatgtc tccgggctga acttcttcag
     2101 ctgattgtag tgctcccggg taaaaccatg ttcatcgcat aaaagttgcc atgtcattga
     2161 tatactgttt gccattcctt gcttcatcat taatacctgt tacctgaata tcaacagacc
     2221 ggtgtacctt ttctgaatac ctccccggac ggagtggcaa tccccattcg cctcagcgtg
     2281 gcatctgcag ctttcgccac ctcgctgata tccagcgttt caggtagcgg cggggacgat
     2341 atctgcgcca gacaataatt ccggcgcaac aacaaccact cccgttgtgc ttctgtacat
     2401 tcaacatttc ccataacaaa aagccagcgg tcagaagctc tgcgccagca accagcagcc
     2461 tccagtttct gcgcaaccat gtcttccaca acttccgggg tactcttttt cctgctcaaa
     2521 acattaactc actgaaataa tatacatatc tgacggtctc agcgaaccgt ccatgccggg
     2581 ctgaaatcct cttcctgaaa cagctccgtc agcccgctaa tctgtttcaa tctgagaact
     2641 tcatcctggt ccatacccag ctccgtacca atgcgttcat tcgtccagcc caggcgcgac
     2701 aggtccctga caatatcaga cattgaggtt atctgatgtt tgcccctggc ccggttatgt
     2761 cggatggtcg cagcaatacg tgaggcctgt ccctttctct ccggattaat acagaccacc
     2821 ggcagccagc ccttcagacg ttttcccgta cctgcttccc tgcccagtac ctggcgatga
     2881 aaaccatcca ccaccaagta atggttcttt tcctcagaca ctaccaccgg ttgtgtgaaa
     2941 ccatctttct ccagtgactg cttcagcagt ttcttctcac ccggcgccat gacattagga
     3001 ttgtaatcat tggccaccac ctcatctgct tttacccaca gcacacagtc cacaggctcc
     3061 tgtgcaaaag gactgacctc atgaagcacc ctgcgcaggt gattaagggc ctgtatggcc
     3121 tcttcttctg gtcgttcctg gctgaagtac tgacgaatca tgttgccaag tatctccggt
     3181 gtcacagaat cccccattcc ttcctccttt ccttcatacg ctgcaggtaa cgttcatagt
     3241 gccggggttt gttagggcta aaggagaggg tccggcacca gaaatcattc tttatgagag
     3301 tcttacagat acgtcgccag gacggaatat cccggctccc caggtcattc tcctgttcat
     3361 ccggaatgtc atccgggaag ccccgcgtct gataccaacg caggtagaca gcaattttat
     3421 tacggtaatg ttctgctgtt ctttccggca tcacatccag caggaacatc gcatagctgc
     3481 gccaggtatg atgaggcggc ttcgtgatac gcttacacag ggcaaaatag gcaccactct
     3541 cattggcata aagcgccccg ctggcagccc ctgacacccg ttcacacatc ctggcccagg
     3601 tttccggctc cagaacatgg taaagccaca gtcccttacg ctgttcagga ccaaaaggtt
     3661 cacagacacg catgttgcgt aacggcacgc cggcacggta catcaggtca tacagaggat
     3721 tgtaaatggc acaggttctg gcgttatata tccagatatc gcgggccttc cagtcataca
     3781 acggatacat ggtgtaataa aacccttcag gcgatgccgt ggtccagggc ttatcatcgg
     3841 catatctcag tttgcgctga gataccagtc ccataaagcg attgagcgat tcatcagcac
     3901 ggacaccagt cagtattgcc actccacacc ggttaccggc aaaccaggaa gaaaatgccg
     3961 gaacaaattc ttcaaacgtc atggcatacc ggtaaaaggg aaaatacgtc atgtctgtaa
     4021 tggcctcttc cggtggctgg cgaacccagg tcactcctgg ctcccagcat atccactccg
     4081 gctgaaactg cgagacgccg tttaccgtgg tcagggaagt gccacccagt aaaaggtttc
     4141 cgtcacatca tggtacattt cccgcatctt ctgaatatgt tcaatagtgc accgatactg
     4201 ggcctcccag tcaatgaaca gaacagagaa atgacgtttt ctcctgcggg ccacctctgc
     4261 cacaagatgg aaaagcacag tggaatcctt tccacctgaa aaagacaggc ataccgacgg
     4321 aaatgtttca aaaatccact caatgcgggc gattgcggca cgcaaaacat tttctccggt
     4381 aaggattttt tcggttaaaa gcgtatcact cattttcctg gctttatgtc gaataattct
     4441 gtttatgcat tcagacaaac ttcattaata actcaccgct ttggggataa ataataacaa
     4501 taaaactctt cagatattaa tctggttgtt ggatttgtta tcacatcctt tatccacctg
     4561 cttcttactg gtaacacatc tggaaaatcc atgcattttc aacatatacc atcctttcaa
     4621 cttgtaaata aggaaatatg atgcagatgg tgaacaattc aaatatcatt ttgcaggaag
     4681 tattcactgt aatagaatta ccccctgata aagtttattc actaaggaat tattcaacag
     4741 aaccattccc tgttagcttt gagcaatagc acttattttt atatgtatca ggaatacgta
     4801 cgttgatgta accaaaacaa agaagcctgt gtccatgggg ttcgtacaat tgtgtgtggc
     4861 acatgaccaa atcaggtttg agacctcccg ctgcaatatt ttttattcag actttaacct
     4921 gtcaggcctg acagaaacgc tcccaacttc ccttctatat cactgtctat agtacgtaat
     4981 cttagcaatg gcaggccgca tttcttcagt atactgtttt taagttcatc acgttcagcc
     5041 tgaacactgg tcagatgata acctccatcc acttcaataa cccctaatgg agtcttcccg
     5101 accttatagt aaacaacaaa gtcacaactg gcccggttac gcatgaataa ctgttcacgg
     5161 tgtgtaaaat ccccccgctc cagcaacacc aactgattca gcgctatttg tgaatgaaac
     5221 atcatactcc gataactatc ctgcgacagg atatcccgca acaggcaggc cacaatctgt
     5281 tctgacttaa agtgtgaatc atttgaattc aatctactat tcagtcgttc cagtgatttg
     5341 tcatactcac tgtacagcaa atcaaatgct gatatgaccg ggctctcgaa aatctctccg
     5401 tcatcagcgt agtatttgat gtaacgaatc agcgccgcaa tatgtccagc gtggcgttca
     5461 aatacatcat taccagtcac cagcgtaaat ttatttcggg cccttgaaac agcgacattc
     5521 accagttcag gattatcaac aaacgcgatg ttccgactgt gctggctgct gcgcttctta
     5581 tccagcacag tggaaaacac aatttcatcg cactcccgtc cctgaaattt atgcaccgtc
     5641 gatttcacaa aatcagcagg cagcactgtt tcagccagat taacctgtgc attatatggg
     5701 gcgatatatc ccctgctact ttcctcatcc cagtaatgtc cttccagaga ttcaagctcc
     5761 ctgagatttg aaaaattacg ggtatggttt ccttttgccg tgatgatcag agataatgag
     5821 gcttcacctg aatcaagcgt tagcggtatc agagcattat cataaaactg cttattacaa
     5881 aattgaatga ttttaggatg acagcgataa tgctctttaa gaagagttac tggcaccata
     5941 ttcctgaaca acatacaaac cgaatcaagt agactatatt tctcacaatt atagtattct
     6001 gccggtggag atgggctgtt cgggagtaat acaggaacat gtggaagttg ctttcgatcc
     6061 cctacaacga ttacattacg ggcacatccc agtcccagaa tgcccggaac aatatcctgc
     6121 tgtgaggctt catcaataat cacataatca agaagagccc ccttaccgat agagttaata
     6181 atagaatgcg tgctgctacc aataaccgga aaacgcttta taaaacggtt aaattcatcc
     6241 ctgtatgtct ctgccgagaa tgaatttctt gttgaaatat ttcttcgaag atgttgtttt
     6301 aaataaagca cagaccatga tgtcaggcgc tccagtaaaa ttttaaaatc agattccttt
     6361 aatacttcct catattcatt cagagaaact tgtttttccc ggatgaattt ttcataataa
     6421 gaaagctgaa gagaagtaaa tagtgccagc cttctctccg gaatcaccaa tggtttgact
     6481 ttcagtatcc tgaagtttaa caacaaatca attctgtttc tgaatccaat ccgcctgtca
     6541 gaaaggtaat gaatataagc catcaggtca gttgtttttc gtgaggaaag tcggtatttg
     6601 tgagaaaaaa gctcctccgg tcgaagattc tgactctgcc attgctgcag gtatttatac
     6661 tcaatattca gctcattaat ttctgctttt aacgaagcga cctggttaat ggcattcagg
     6721 tgttttttca cttgttgaag tacatcatca atcgcatttg cagacggtga gtcaggtaag
     6781 acttcctcag aacgcgaaat cgacgttgaa aaaaactgtt gcctgttctc cgtactcccc
     6841 agtcgtgcca ccacatatcc cagttgctgt ttgtccatct tttcataaac attactgaca
     6901 gctgagttat tattggacaa aattgccact gttttattct gaattaatat attagcaaca
     6961 atattgagta tagtctgtgt tttacctgtt ccaggtggtc cttcaataac tgaaacctgt
     7021 gaggaaaatg cccgttcgac agccagcaac tgactttcat taagaccaaa cggaaaaatc
     7081 aaaccccatg gcgaatcacg ttttttactc tgtcctgaac agtatgcatg caaggcggta
     7141 tccggatgag gaactatttt ctttatctgc ctaaggatat tctcatcgat atttctgtta
     7201 ttaccgcggg cataaaaaat acgttcatta gccaccctac acaagtaatt aaacacggca
     7261 tgttctgtaa atgctgaaca ctgttgaaag tcaagaccgg tcgttttcat gatgtagggt
     7321 cgttcaccct cgcgataata aacagcagca tatttatcac catatataat aaccagttca
     7381 gcccgggatg ttacagcgtt ccctttcctt tcgaggaaaa catttcgcaa atttacagtt
     7441 ggcgttatct gacatacaga aagcggacga gtatattttc tcccgctgcg aaaatgacag
     7501 gtcagcataa cctctctgtc gtcatttcca gcccacagcg tccagtcggt aattttatga
     7561 gtcttatttt caccgtcaac acaaatcgtg accatattct tactcagaaa ttatcattta
     7621 acttacggaa aattgttaag gcactcccga tattagctcc ggaggatttt ccacaactcc
     7681 gggactgtta acatatttct gcctgccata tatacattga atttagtcat aacagtcaat
     7741 accagaatta caggaatgcg atgccgatac gctggttatt gaatactggt agcccctgag
     7801 cactaattca tcacgatact ctgtatttac tgttttcact gctttttaca accattcccc
     7861 caggtaaaat gctgctcctc cggtgatggc tgtgtcagaa actgccatcc cggaatagcc
     7921 acgctgaccg ccagcaatgc cgcacagaca ccagccacaa acggtttcag aaacgtgggc
     7981 ggggatttct gaagggaggg tgactgctcc gcctgcactt tccccttgcg attatcaagg
     8041 ctcagtgcat ccagacgcac cgcgccattt gccgcaagca gcccttcccg gttatccacg
     8101 cttcccggca gactcagcga aaaatcccct gttcccgtct gaatcagact gcctttacga
     8161 tttgacaggc tttgtgccga tatctgcagt gtgtcggcat ttatcgtacc gccattactg
     8221 ctgaactgcc cggcagtccg ggcggaaagc gtatcggcac tcagttgcgc attctgtgta
     8281 gacagatttc cactggttgc attcagctca gtatgttgtc cctgtatccg gctgttacct
     8341 aaatccagcc cctgctcaat gtcccgtaaa tccagcagag aaccttcgtg tgccgggaat
     8401 gaggaataca actgaatata gtcatcactg tccggtgtca gacgcacatg tcgcacaaca
     8461 ccgggaataa taaccagccg gaggataccg ctttttaaat cctgcgaagg tgccagaaca
     8521 cgggtggtga cataaccatg atcgaccaga cggttctgca atgtactcat cagcagatta
     8581 attcctttcg cccccaggca atgcccgacc gccccgttgg cgattttttg taaaggcagc
     8641 cagtgtggta acgcatcagc ccccttcagt tccgtctgtt taatctgaaa acagggcgtt
     8701 tcaacaggaa aattaatctt atgggcaaaa gagccaggtg cagaaaggcg aacatcaggg
     8761 gctgaaggcg tcagttgttg ctcaagggct tcctgacgct gctgctgaat gataaatgct
     8821 tcatctccgc tacgccggac atccgctgcc tgaagagata aaggcgataa taaagcagac
     8881 atcagtgtca gtgacagcaa ggacggacag cgccggaata agctttgttc ctcagtcctc
     8941 tcgttcctgc aacataattt tccagtcaaa cgaaaaacct tttattcatg tcataccaac
     9001 aatcaccagc ctcctgacga tacctcccac catgttttgc gatggcacgc taccgccctt
     9061 ccggtattta cccgcatcaa ccggtaccag aatgctgagt aacaaaccag ctagaattga
     9121 cttaattttg atcattatag aataatgatc tgaactacag caacatgaca gatttatcga
     9181 tcgat
//
LOCUS       DAZKGS010000167         9152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKGS010000167 DAZKGS010000000
VERSION     DAZKGS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..116)
                     /locus_tag="ACHOQY_004035"
     CDS             complement(<1..116)
                     /locus_tag="ACHOQY_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680035.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNSRDIRNIKNFSSHQHS"
     gene            313..1049
                     /locus_tag="ACHOQY_004036"
                     /pseudo
     CDS             313..1049
                     /locus_tag="ACHOQY_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1228..1524
                     /locus_tag="ACHOQY_004037"
     CDS             1228..1524
                     /locus_tag="ACHOQY_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9680036.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1832..1948
                     /locus_tag="ACHOQY_004038"
     CDS             1832..1948
                     /locus_tag="ACHOQY_004038"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680037.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2167..3600
                     /locus_tag="ACHOQY_004039"
     CDS             2167..3600
                     /locus_tag="ACHOQY_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9680038.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4421..4984
                     /locus_tag="ACHOQY_004040"
     CDS             4421..4984
                     /locus_tag="ACHOQY_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9680039.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            5139..7499
                     /locus_tag="ACHOQY_004041"
     CDS             5139..7499
                     /locus_tag="ACHOQY_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9680040.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(7661..7837)
                     /locus_tag="ACHOQY_004042"
     CDS             complement(7661..7837)
                     /locus_tag="ACHOQY_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680041.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(7975..8217)
                     /locus_tag="ACHOQY_004043"
                     /pseudo
     CDS             complement(7975..8217)
                     /locus_tag="ACHOQY_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(8256..>9152)
                     /locus_tag="ACHOQY_004044"
     CDS             complement(8256..>9152)
                     /locus_tag="ACHOQY_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP9680042.1"
                     /translation="RSLLSGWVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLP
                     GNKKTGRLWAYVRDDRNAGSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGF
                     NELYRNGGITEAACWAHARRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQ
                     RLAERQRKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEI
                     DNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGV
                     IADWPVNRVSELLPWRIALPAE"
BASE COUNT         2492 a         1904 c         2188 g         2568 t
ORIGIN      
        1 gaatgttgat gtgaggaaaa atttttaata tttcggatgt cacggctatt ctggttttta
       61 gccagaacac caaaacatga aaagacatca tacattatag tgataaaatt atacataaca
      121 ttctcctttt attgcactat ataaaatttt aacattatat cattttgctt ttctgttaac
      181 tggttttcat ttttgaatca atattttctg aacagggtaa tagtacacgt attaacagat
      241 tatgtcagaa ggccccggtg atgccttctg acgatagatt aatgttgtat tctattggta
      301 tctggagacc atatggcaac agttacagta tattctcccc gctgtaattc ctataaagtg
      361 taccgttatg atcacagcac ctcagatcat gagcgtttcc agtgctaatc ctgcaaacgt
      421 gtatttcaac tgaattacat ctacgaaacc cgaaaaccag gcattaaaga gcaaattgtt
      481 gaaatggttc ataacggagc cggggttcgc gatagctcca gaacgctgaa ggtggatatc
      541 aacaccgtca ttctcacttt aaaaaacgct caccacgtaa agtaaccata tctccagccg
      601 ccagtgtcga tgtggcactt atctgtgaac ttgatgaaca atggagtttt gtcgaaaaca
      661 aagctcgtca gcagtggcac tggtacgcgt ataagaccaa agctgacggt gtgctggctt
      721 acacttttgg tcctcgcact gatgaaacat gccgtgagtt gccggaattc ctgaagccat
      781 tctccgcagg catgataacc agagacaacc ggagcagtta taccagagaa atgccacagg
      841 ataagcatct tgtcggcaag atatttaccc ggcgcattga gcgcaacaat ctgacacttc
      901 gtactcacat caaacgacct gcccggaaaa cgatttgctt tttacgctcc ctggaaattc
      961 acgaaaagcc attggttcat ttatcgaaaa cacatgtact actgacagga gtcattacca
     1021 gggcctcttt tgctgttttt ttgccatgaa tgataatgag aatcgacagc gataagggaa
     1081 aagtaaaata acactaatgc tgtcgttttc tatcatattg atttttaatt atttattgtc
     1141 ttttttctca acccattttt ttacgcagac aaatttaccc ggggaataca aactgtaacg
     1201 acagttcacc acactatggg ggataaaatg ccagatcaat ccggacactg caggactcct
     1261 tcacgcatct tacggatgcc tgaactgagc caattgttag gtatatctcg ttcgacaatt
     1321 tacgaaaaaa tgaatccgtt atcgaaatac tacgatgcaa ccttccccag gccggtacgt
     1381 cttggtagtg gttctgtggg ctggcgttca tcagctattg acgaatggct tactttacat
     1441 actgtacctg ccagatcagc agtcaaaggt gttaatgacg aagtgagaca tacgctttct
     1501 tcagatatga ccatgagcag ataagttttt gtcatcacgt gatcacctca ggaggaaaaa
     1561 gaagcataag aacaatttag ccagaagttt aattgataaa cagaatttcc agcattcatt
     1621 gttgaaagag cagctattgt cgctattgta gcggtaattg ctgctataac cgaaacgtat
     1681 tacgaaaaag gaagcattta tgccttacat caaaccggat atgtcgtatt cagtatatgc
     1741 atgaccatca atgataggga aggcaataat taaagctcaa tacaataaac aagtacctct
     1801 gactctggtt gcaatgtcag tattaacagc gatgtcaatt gcctgtcaga atcaggttga
     1861 cgtatgcagt cccggaaatt tacgtggggc cgttaatatt tacactatgg ttctggagaa
     1921 agggaaaaga ccgggggaaa ggtattagtg aaataatttc cggaggtatt aagaatgaga
     1981 aatatgaatg atatttcgtg gtgaataata cagtcttcac tgaattaacc ttatcggttt
     2041 attaaacatc agatctacaa atgctctgta tttctcagag catcaggtct gaatagcagc
     2101 tattttcgct attgtagcgg gatttgctgc tattttacta acccattaat gaaaagagag
     2161 gcttttatgt gcttacttgc accagaaaat ccttatccga tatatgcact accaccgctg
     2221 gtgagaaatg caataattga aactcaaaaa aatacacagg cacctttggc tatggtggca
     2281 acatccgcat taacagcgat ctcaattgcc tgtcagaatc agattgacgt gtgcagacct
     2341 ggaaatttac atgggcctgt taatctttac tctctgattc tggctgattc cggtgaaagg
     2401 aaaacgaccg tggataaagt gtttatgaaa gcattttatc tcagggatga agccctggcg
     2461 gatgaatacg cgaaactggt tgagaattac agtacagaaa aggaaatatg ggagcaaaaa
     2521 caaaaagcgc tggaatcaaa atttcataaa gagattcgtg ccggtaaaga ttacaaggca
     2581 acagaatcag agcttgaaac gcatctgaat aaacctcctg ttccgccgca gatacgtcga
     2641 acaattttta atgagacaac gatagaggga atgttaaaat attactccga tagcaatcgc
     2701 tcttttgctc ttgtatccag tgaagggggg gtaatttttg acagccgggc catgagtaaa
     2761 ctgggaatta ttaacacttt gtgggatgga ggttctcttt tcatcgacag gaaatcatct
     2821 cccggaatta atttgaagga gccaagactg acgatatcgg tgatgattca gcctgatgtt
     2881 taccacaaag gtttttgtac gcgaaaaaaa gaaattgtga aaacgtcagg acatcatgca
     2941 aggtttttga tgtgtcaacc aacatcaacg caggggacaa ggataataac cggagataat
     3001 tattcatcac agtatcagga tttgtttgaa gagaggatca atgagcttat tgatgaaagt
     3061 ctggcgatga gtggtgaacg acgttgcctt cacttttctc ctcaggctgc cagaatctgg
     3121 acggattatt acaatgatgt ggaatcgaag ctggggggat tggggccttt aagacattgc
     3181 cgggaatatg ctgctaaaaa tgcagagtat atggcaagac tggcaggact tatttaccat
     3241 tcgagcggtg aagaggggga gatttcccct tacactgcag aaatggcgag agaattagca
     3301 atatggtacg gtaatgagta tgtgcggttg tctaatccgt taacttttga caactctgcc
     3361 ctgaccgtac ctgtgcgact cattccagag gagcttgaac ttttcaactg gataaaaagc
     3421 tattgtattg agaaggggat cctctgtatg aaaaaaaatg acattttaca gcgtggtccg
     3481 aaccgtttcc ggaaaaagga taaaatcaac tggttactgg atttactgta tgaacaaaac
     3541 agagttgtac cggttattga gggaaaaacg ttgtgtgtcg cacctaactt tgacctctga
     3601 acgacttaat cgttgagtac atatagtctc ctctgctact tgcggaggag actaatgtct
     3661 ttaccatgtc agcgttgtat tagaaaactc tggtacaggt cagtcagtaa atataactgg
     3721 tgtaaccact atttactatt ggatagagtg ttactcagga tattgtcggc tttagtctga
     3781 ggtgaggtgg ggccttcatt gttttcgtgt tcaataaata atttaggtta attagtatta
     3841 ttagtggaag ttatgtgatc tgttatgttg tcttgtacgc acttaacact ggttacgtac
     3901 cggaacacta ttagagtatg ttatggctct gttacagttt ataggtatga aaaaatataa
     3961 tctttcctgt atgaaataat attcttatgg tgtgtggggt atggtctgta taccatcatt
     4021 tttatggtga agttaaatat ctgtagaggc ctcaagctgg gtcgctgttt ttacttaatg
     4081 gtacagaagt attaatagcg gtgttgcctg tggctacccg atactggctt ttgtgaatgt
     4141 cagattactt cctgtaagtg attgaatggt acttccttaa actcaattct atgaggtgtg
     4201 tttttaccat tgtaattcgt actggtcctc agtttaagtg agtcttactg tactctgaaa
     4261 tattacctct ttggagtaat gaggtaatac gaaacagttt tatcaggttg tctgtgctgc
     4321 tggttacctc gtttattaat gccgcggtac ggcgtttctg tactctcagt atttatgtaa
     4381 ttccaccttc ctgtaacttt tataaagtaa ggactataaa atgtataatg ctaatcccaa
     4441 ttacgaaatg gacttcatga ttctgaaaga cgtgaatgaa cacatggaag gtctgtttca
     4501 acgtttttct aaactgttac ctttcagaat tgattttgcc tacaggaaag acacgcccag
     4561 ttttggtcat tcctgtaggc acagtatgtg tattgaaatg caccgcttac ttagtgaaac
     4621 ccaaacgatg ctggcgggat attactgggt aatggaatat acgtcaaaca agggacttca
     4681 tattcatttt atcggctacc tggatgggca acgtcacaac aaatcttatc ggatatccag
     4741 gcaactggga gatatctgga ggcggatcac ggaaggggag ggttattttc acctgtgcag
     4801 ggctaaagac aaatacccgg tgcgtatcga tcacgtaatt cactatagtg ataaatctgc
     4861 ggtagacgac ttacgttacg cccttagcta tctggccaaa caggatcaga aagaacacgg
     4921 tattattctg aggcgtagca ggcttcccga aaaaagtaac cggggccgac cacgacataa
     4981 ctgaattaca gctgtattag gtattagaat taagtttctg taagttgttg ggaggtgctt
     5041 gagttaatag gcagaagagg gcggggcgct ttgtcatcag tgtaggggac ccgaatttct
     5101 gccgtggtgc ctcagtggat cggatacagg taacgaatat ggttcataaa tctgacagtg
     5161 atgaattagc tgcattgagg gcagaaaatg cccgtctggt ctcattactt gaagctcatg
     5221 ggattgaatg gcgacgtaaa ccgcagactc ctgtgcagcg cgtttctgta ttatccaccg
     5281 acgagaaggt tgcattattt cgtcggttgt ttcgtgggcg tgatgatgta tgggcactta
     5341 gatgggaaag taaaaccagc ggcaaatcag ggtactctcc tgcctgcgct aacgaatggc
     5401 aggcgagaat atgcggtaaa ccccggataa aatgtgggga ctgcgctcac cgtcagttga
     5461 ttcctgtatc cgatctcgtc atctaccacc atctggccgg tacccatact gtcgggatgt
     5521 atccgttgct ggaagatgat tcctgttatt ttctggcagt tgattttgat gaagctgaat
     5581 ggcagaagga tgcatccgca ttcatgcgat cctgtgatga gctgggtgta cctgctgcgc
     5641 tggaaatatc ccgttcacgt cagggggcgc atgtctggat attttttgcc tcacgagttt
     5701 cggcccgcga agctcgccgt ctggggactg ctattattag ctatacgtgt agtcggaccc
     5761 gacagctgcg attggggtct tatgaccgat tatttcctaa tcaggatact atgccaaaag
     5821 ggggatttgg taatctcata gcgttacctc tgcaaaaaag accgcgtgaa ttggggggaa
     5881 gcgtttttgt tgatatgaat ctccagcctt atcctgatca gtgggctttt cttgtatcgg
     5941 tgaccccgat gaatgtgcag gatattgaac cgacgatatt acgggctaca gggagtatcc
     6001 atcctctgga tgtgaatttt atcaacgaag aagacctggg tacgccgtgg gaagagaaaa
     6061 aatcatcagg aaacagactg aatatttcta ttgcagaacc gctgaaaatc acgctggcaa
     6121 accagatcta tttcgaaaaa gcgcaattac ctcaggtgct gattaaccga cttattcggc
     6181 tggcagcatt tccgaaccct gagttttata aggctcaggc aatgcgtatg tcagtctgga
     6241 ataagccccg tgttataggt tgtgcggaga attacccgca acacattgcg ttgccccggg
     6301 gatgtctgga cagcgtatta tctttcctta gggacaacaa tattgctgca gaattaatcg
     6361 ataaacgatt tgccgggacg gaatgtaatg ccgtttttat gggaaacctc agagcggagc
     6421 aggaagaggc cgtttcggca ttactccgtt atgacactgg tgtgctttgt gcgccaacgg
     6481 cttttggtaa gacagttacc gcagcggcag tgattgccaa gcggaaagtg aatacactga
     6541 tactggtaca ccggactgaa ttgctgaagc agtggcagga gcgtctcgcg gtgtttctgc
     6601 aggccggtga cagtattggt attatcgggg gaggtaaaca taaaccctgt ggcaatattg
     6661 atattgcggt ggtgcagtcc atatccagac aaggagaagt tgaacctctg gtcaggaatt
     6721 atgggcaaat cattgtggat gagtgccatc atattggcgc ggtttcattt tctgcgattc
     6781 tgaaggaaac gaatgccaga tatctgcttg gcctgacggc aacaccaatc cgacgggatg
     6841 gtctgcatcc cattattttt atgtactgtg gtgccattcg ccatacagcg gtccgcccga
     6901 aggaaagccc acataatctg gaggtactga tccgttcccg ttttacatct ggtcatttac
     6961 catcggatgc gagaatccag gatattttca gagaaattgc tctggatcat gacagaacgg
     7021 tggcgatagc tgaagaagcc atgaaagctt tcgggcaggg gcgaaaagtt ctggtactga
     7081 ctgaacgtac agatcatctg gatgagatag catcagtgat gaattcactg aaattgtctc
     7141 cctttattct ccatggtcga ctatcgaaaa aaaagcgtgc gatgctgata tccgggctga
     7201 atgctcttcc tcccgattct cctcgaattt tgttgtcaac aggcagactt attggtgagg
     7261 gatttgacca ccctccgctg gatacgctga ttcttgccat gcctgtgtca tggaaaggga
     7321 cattacagca gtatgcaggg cgtcttcaca gagagcatac cggtaaaagc gatgtcagga
     7381 tcattgattt tgtggatacc gcgtatcctg tgctgctcag aatgtgggat aaacgtcagc
     7441 ggggttataa agcgatgggg tacaggatta tagctgacgg cgatgaatca gtgatttaat
     7501 tttaaggagt actttaccct ttttaaataa tttgagctcc ccgggttgtt cgggcaacaa
     7561 agagtcccag tatacaggct tcaggaatat gttgtagtat caggcttttc atcgctttaa
     7621 aatgactacc ggcagtgaga acgtcatcaa aaataacaat ctagggcctg cacccttcca
     7681 gtaattgttc atccagttgg tagtttgcca taatcgcttc tggtaagggc cggtttgttg
     7741 cggtatgaga ggctattgtt gatacgtttt gcacaaccag ttcccagaaa tccagccatg
     7801 gttcgtaggt ttgcgcaatt cgcagaattt ctgtcatacg gtcatcgtaa gcggcatctg
     7861 tacggcattt ggatggtggt acgggaacaa atgtgtattc ctcaagatta ccgatatttg
     7921 atatgagcat ctgagcaaca tactcaatag cagccttttt atagtggtaa tcaggctacg
     7981 aaagtacagc gttttataaa ttgtctcagg tgatattcgc agcgtttttt gacgtggttt
     8041 tgttcgcctt aaccatcctg atatttgctc tggagaccat ttcatctcca gcttttccag
     8101 aacaagcttt cgcaatggta aattttgatc cagtaagcac ggttttggcc ttttcgccat
     8161 tctgttggct cggttattag catcaacagc tttgtaatag cgtctgcccc gattacgcgt
     8221 aagcgtacag cgagggccgt attgacgggg atgtgttatt cagctggcag tgctatgcgc
     8281 cacggaagca gttcgctgac ccggttgacc ggccagtctg ctatgacgcc aagcacatgg
     8341 cgaaggtagc tttctggatc cacgtcattc agtttgcacg tcccgatcag gctgtacagt
     8401 agcgctcccc gctcaccacc atgatcagag ccgaagaaca ggaagttttt acgacccaga
     8461 ctgaccgccc gcagggcatt ttcagcgatg ttgttgtcga tttccaccca gccatcgttc
     8521 gcatagtacg tcagtgccgg ccactggtta agtgcgtacg cgaacgcctt cgccaactct
     8581 gagtgtcgcg acagggtctt catcttttca cgcaaccagc tttccaggga tttcaacaac
     8641 ggtttcgttt ttcgctgacg ttcagcaagc cgctgctctg ccggcattcc ccttatatcc
     8701 gcctctatgg cgtacaactg accgatctgc tccagggctt cttccgtcag tgctgacggg
     8761 atgcggacgt gcacatcgtg gatctttcgg cgggcatgag cccagcaggc agcttccgtt
     8821 atcccaccat tgcgatacag ctcgttgaac ccggcgtacg catccgcttg cagcacaccg
     8881 ctgaagcagg caagatgagt ctgcggatgg atgccttttc tgtccgggct gtaagcgaac
     8941 cacactgcag gtgccaacgc tgaccctgca ttgcggtcat cacgaacata cgcccacaac
     9001 cgcccggtct tcttattacc cggcagcagt acctggaccg gggtatcatc ggcatggagt
     9061 ttgccgtcag tcatgacata gccatgaagc gcctcttcca gcggagacag cagccggcag
     9121 catgcatcca cccagcccga cagcagtgaa cg
//
LOCUS       DAZKGS010000168         9127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKGS010000168 DAZKGS010000000
VERSION     DAZKGS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1195)
                     /locus_tag="ACHOQY_004045"
     CDS             complement(<1..1195)
                     /locus_tag="ACHOQY_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFP9680043.1"
                     /translation="MIHPGSFLDKTINNTRGKNVNANIKHSQIQEIKRNFTYKKNDDI
                     SSRFKFYSSLVKQKNSTEDVVLIGKMISDEVRNYSTIHNDRNIIINSGNWKTSFLCNL
                     ARLLYSMFNGSVYFPSRDDENSSSPSPTLPTIHQSERKELSQQKSINHLKKSDTIDEY
                     IKIRKTRGIEDQITTTSQSLIINELLNGVNRNTIPFQKISELNDIIHSYENMQTKNSR
                     KGIEILVKQGELLSSLINYDKENKQLSDNISKIINLLGIEYQSHKVDIEPFIHAVWVA
                     GAPPENTSSYITAFLNTYKNYTYLLWIDPNAFGAAKFSGILKNIAMNYAIMRLRRTNP
                     HLAEKMNDVILKIQHIQNETVDFNETRERVKELENKYKSLTRETKEKFNVFFLESMIG
                     MQDNYF"
     gene            complement(1310..1540)
                     /locus_tag="ACHOQY_004046"
     CDS             complement(1310..1540)
                     /locus_tag="ACHOQY_004046"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9680044.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
     gene            1463..1955
                     /locus_tag="ACHOQY_004047"
                     /pseudo
     CDS             1463..1955
                     /locus_tag="ACHOQY_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2022..2150)
                     /locus_tag="ACHOQY_004048"
     CDS             complement(2022..2150)
                     /locus_tag="ACHOQY_004048"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFP9680045.1"
                     /translation="MFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPE"
     gene            complement(2181..2947)
                     /locus_tag="ACHOQY_004049"
                     /pseudo
     CDS             complement(2181..2947)
                     /locus_tag="ACHOQY_004049"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2983..3522)
                     /locus_tag="ACHOQY_004050"
     CDS             complement(2983..3522)
                     /locus_tag="ACHOQY_004050"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9680046.1"
                     /translation="MLTTSESQFRQKDQQLSLVEETEAFLCSALARAEEKIEEDEREI
                     EHLQTQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDAPQVPRQLRQ
                     PRHRRPLPAHLPHAIHRLESEESCCPECGGELDYLEEVSAEQLELVSSALKVIRTVRV
                     KKPVQNVTASLKHRRRPAR"
     gene            complement(3534..4686)
                     /locus_tag="ACHOQY_004051"
                     /pseudo
     CDS             complement(3534..4686)
                     /locus_tag="ACHOQY_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            complement(4667..5143)
                     /locus_tag="ACHOQY_004052"
     CDS             complement(4667..5143)
                     /locus_tag="ACHOQY_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9680047.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            5230..5344
                     /locus_tag="ACHOQY_004053"
                     /pseudo
     CDS             5230..5344
                     /locus_tag="ACHOQY_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5395..6904)
                     /locus_tag="ACHOQY_004054"
                     /pseudo
     CDS             complement(5395..6904)
                     /locus_tag="ACHOQY_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7524..8531)
                     /locus_tag="ACHOQY_004055"
                     /pseudo
     CDS             complement(7524..8531)
                     /locus_tag="ACHOQY_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2180 a         2280 c         2049 g         2618 t
ORIGIN      
        1 tgaaatagtt gtcttgcata cctatcattg actcaagaaa aaaaacattg aatttttctt
       61 tggtttctct cgttaatgat ttgtatttat tttcaagttc ctttaccctc tctcttgttt
      121 cattaaaatc tactgtttca ttttgtatgt gttgtatttt taatatcaca tcattcattt
      181 tttccgctaa atgaggattg gttcttctca aacgcataat tgcataattc atggctatat
      241 tttttaaaat tccactaaac ttggctgcac caaaagcatt gggatcaatc cagagcaagt
      301 aagtataatt tttatatgta tttaaaaaag ctgttatata tgacgatgta ttttcaggag
      361 gtgcacctgc aacccatact gcatgtataa atggctctat gtctacttta tgtgactgat
      421 actctatccc taataaattt attattttag atatattgtc tgataattgt ttgttttctt
      481 tgtcataatt tattaatgat gataacagtt ctccctgctt aactaatatt tctatacctt
      541 ttcggctatt tttagtttgc atattttcat atgaatgtat gatgtcattg agctcactta
      601 ttttctgaaa tggaatggta tttctattaa ctccatttaa cagctcatta attatcaaac
      661 tttgagaggt agttgttatt tgatcttcaa tgcctcttgt ttttcttatt ttaatgtatt
      721 cgtcgatggt atcacttttt tttaaatgat tgatgctttt ttgttgtgat aactccttcc
      781 tttcagactg atgtatagta ggtagagttg ggctggggga tgagctattt tcatcatcac
      841 gggaaggaaa atagacgcta ccattaaaca tgctatatag tagtctggca agattacata
      901 aaaaagatgt tttccaattt ccagagttaa ttatgatatt tctatcatta tgtatggtgc
      961 tgtaatttct aacttcatct gaaatcattt ttcctatcaa aacaacatct tctgttgagt
     1021 ttttctgctt tactagcgag ctgtaaaatt taaatctgga tgagatgtca tcattttttt
     1081 tataggtgaa attccttttt atctcttgaa tttgactatg tttgatatta gcattaacat
     1141 ttttacctct tgtattgtta attgtcttat ctaaaaaaga acctggatga atcatatgta
     1201 tactctctag ttacgcaaca attaatgaat tttgattggt gacaacctca aatgtatttt
     1261 gaggttgtta tataaaacac attattttat atgatttggt gatatccaat tagtagaaca
     1321 tgtatttttc gataaagtac cggtgttttt ttatgaaatt tacagagtgc gggaaacaga
     1381 tagccctacg ggtcagatgt ttgattaata ttctcatgca ctcaatatgc tgaataaaaa
     1441 tagatgagtc tccttctata acatatctga tgtgactacc ccattcgtca gtggtcaata
     1501 taccaatgaa aaaaagaggg aagcagcgcc agcaattcac aacagtatag gtattgctgc
     1561 tgcagcccgt gagctcagcc tgtatgaatc acagtctaca actgggtagt caacaacaaa
     1621 atcagtagac gttcttctga acgtgagctg gagatgtcta ccgatgttgc acgtctcaaa
     1681 cgccagctgg cggaacggga tgaagagctg accatcctcc aaaaggccgc gacatacttc
     1741 gcgaagcgcc tgaaatgaag tatgtcttta ttgaaaaaca tcaggctgtg ttcaacatca
     1801 aggcaatgtg ccatgtgctc caggaaggcc gcagtgactg gtatatgtgg tgtcagcggc
     1861 ggacaaggat aagtacatgt cagcagttcc gtcagcactg cgacagcgtg gtactcgcgg
     1921 cttttatccg gtcaaaacag cgctacggag caccgtattg acgcttattt attggtgagt
     1981 actacgttcc atggcaggag ttcgtcaaca cggttggagg gctattccgg tagtacgctc
     2041 agaatatggc gcagatacgc ttccggatcg ataccgttca gacggcaggt gccgatcagc
     2101 ccgtacagca gtgctccacg ctcgccgccg tgatcgctac cgaagaacat gtaattcttt
     2161 ttcccgagac agacggcacg aagcgctctt tccgctgtgt tattgtccgc ctccgccaga
     2221 ccgtcatcac tgtaataaca gagggcgtcc cactgattca gtacatagct gaacgcttcg
     2281 cctggtctgg attttttcga cagcgtgcca ttcttctcca ccatccattc atgcagcgac
     2341 gtcagtaacg ctttgcttcg ctgctgcctg acttcaagac gctctgactc tggtaatccc
     2401 cgtatttcat cctcgatggc gtacagttca ctgatgcgct tcagggcttc ttctgccgtc
     2461 gcacttttgc tgctgatgta tacatcgtgg atttttctcc ggacatgggc tcagcacgca
     2521 acttctgtca gcgcaccatc ttcacgttct gcactgaaca gccgatcgta accgttgaac
     2581 gcatccgcct gcagggggcg gaggtgttgc tccgggtgtt tcccctgccg gttcggtgag
     2641 tacgcgaacc agaccgccgg aggagatgac gaacccgcat tgcgatcatt ccggacatac
     2701 gtccagatgc gccctgtttt cgtcttttca ggcccagtgc cagtaccttt accggtgtgt
     2761 cgtcagtgtg aagcttgcgg gtattcatca cataacggta cagggcatca ttcagcggag
     2821 tcattaactg gcagcacgcg tcaacccagt tggagagtaa tgcacggctc agttcgacac
     2881 cctgtcgggc aaagatttca ctctgacgat acagtggcag ctgttcgcag tattttcccg
     2941 ttaacacgcg ggcaagtaat ccggggcccg cgataccacg ctctatcggg cgggacggcg
     3001 ccggtgcttc aacgatgcag tcacattttg tacaggcttt tttacccgaa cagtgcggat
     3061 cactttcagg gcgctgctca ccagttccag ttgttctgca ctgacttcct ccagataatc
     3121 cagctcaccg ccacactccg ggcaacagct ttcttcagac tccagacggt gtattgcatg
     3181 gggaaggtgt gccggtaacg gacgacgatg gcgcggctgt ctcaactggc ggggaacctg
     3241 tggagcgtct tcccgcccac tgtaacgatc gctgtcctgt tcgcgttgtt tcagcagggc
     3301 ctcagcctgt tcaacttcac gacgcagttt ttcagaacgg gtaccgaaca gcatccggcg
     3361 cagtttttct atctgagtct gcagatgttc tatttcccgt tcatcttctt cgatcttttc
     3421 ttcggcacgt gccagtgcag agcacaggaa ggcctccgtc tcttcaacca gacttagttg
     3481 ctggtctttc tgacggaact ggctttctga agtggtcaac aaaaactggc cacctagtta
     3541 gagtttttcc agtatcgatt ttccgattcg tttgggggta acccaccgtt atattcgtgc
     3601 ggtcttagtg cgctgtaata tccaacgata tagtccgtta tggcgtgagc tgcctcgctg
     3661 aagcttacgt aacccaccac cggcatccat tcgttctctt cagactcctg aagaagcgtt
     3721 ccattgggct gttatcccag cagtttccgc gccggctcat actctgtctg atctggtatc
     3781 gccacaataa ctgccggaac tgcctgctcg tataatgact gccctgatcg ctgtggaaca
     3841 tcaccccgcc gggcttacca cgggtttccc atgccatttc ccgcgctttc atggtgagcc
     3901 tgctgtccgg cgagaacgac atggcccagc ccactggttt tcttgcgaac aggtcgagaa
     3961 caacggcgag gtacgcccag cgcttacccg tccagatata ggtcacatca ccgcaccaca
     4021 cctgatttgg ctcggtcacg gcgaactgcc tttcaaggta gttagggata gcaacatgtt
     4081 catgaccacc acgtttatac cggtgagtcg gctgctgaca gctgaccagc cccagctctt
     4141 tcatgagcct gccagcaagc cagcgtccca tctggtagcc tctccgggtt gccattgtgg
     4201 cgatgcttct tgctccggcc gaaccgtggc tgatgccatg tagctcaagt acctgactgc
     4261 gtaatacagc ccgtctgccg tctggttttt caggacggtt tttccagtat ctgtagctgc
     4321 tgcgatgaac cccgaacaca tggcagagtg tgaccacagg ataatgcgct ctgagtttcc
     4381 cgattatcga gaactgttca gggagtctga catcaagagc gcggtagcct tttttaatat
     4441 ttcattctcc atttcaatgc gttgtagctt tttcctcagc ttacgtattt cgatttgttc
     4501 tggtgttatc ggagaggctt ttggtgtttt gccctgacgc tcatcacgca gttgtttgac
     4561 ccatcttgtc attgtagaaa ggccaacatc catagctttg gcggcatctg ccaccgtgta
     4621 tttctggtca acaaccagtt gagcggattc gcgtttaaac tctgcgctaa aatttctttt
     4681 tttcattaga gcacctgtgt tgttctgagg tgagcatatc acctctgttc aggtggccaa
     4741 attcagtgtg ccacttcaca gtgagcagct ctggtgaagg attttccagc gtcatgttac
     4801 cgtgacggaa ctccactttg caggagctgg cactgacagt agtctgagtg gataaggatg
     4861 gagtaagagc agccatcggt tctttcggct catcaggcgt tatctctaca ggtaataatt
     4921 caacgcctgc gtcagaagtg gttgtcaccg gaagacgccg tgatatacgc ccttcgtttt
     4981 gccagagtct gagccatttg aaaataacat catcattgac gccattttct cgtgcaatct
     5041 gggccataca agcaccgggt tgtgatgcca gttcaaccat acgaagtttg aattcattcg
     5101 aatacttttt acgaggttct tttcgccagt cctgtaattc catacttgga tgtccgtctg
     5161 tgtcagatgg gcgtctaagt taccaattct tgtctaatgg ctacatactg cggtcggttt
     5221 acgcttactt ttaacgtaaa aaccgtgatg gcaagcctgt gccgtcacgg actgagggca
     5281 aaggcttccc ggaagttcag cccttaccgc acacagcctg cctgtgtcag aaaatctgct
     5341 ggagctgtca acgacggatg aaaagtgcct cttccgcaac ctgggtggag attttcatgc
     5401 cagtggactc attaccctct gcctgagtag ttcaaatcct gctcgaccat acatctggcg
     5461 tttcagcatc ttcaggcgat ttacatgacc ttcgacaaca ccattactcc agcgactact
     5521 gattgcctca catatagctg cagcatcagc ttccatcccc gccgccactc gccgaaattc
     5581 tgctgaaccg ctttcgtgga ctcgagtgaa ccaactactt agctgtgatt tattttgggt
     5641 tttcagaata cggtagaact cgagtgccag ttgctgcgct attttcagct caggttcttt
     5701 tcacacatca gactaataaa tcgggaagca taattttctt catccctggt tattctccag
     5761 ggcatcaacc agcggcttac ccgggccacc atttcccgcc atatccggct ggcgttataa
     5821 ttaccacttt ctcgttgctc ttttaaccac tccctccatg ggtccagaag ccctcgcttt
     5881 ggaggtcggg ttgacatttc agggaatgtt cctgaacgca cccagcgact gactgtcaca
     5941 cgcgacaggc ctgtaatacg ggatatttcc ctgaaactac atcccttgtt atgctgggcc
     6001 ataacctctg tccaacgctg atgccgtttt ttgcgggttt gttgttttag gcgttccgga
     6061 cgacggagcg atgctgcagg ctcagacagt tctggctcag gtggtttctt tggtgacaac
     6121 tcgctggcaa caagacgtat cagaggtata tgtctgtaca tcattcgttc aagcgcatcg
     6181 ccaatatttt ttagcaggtg gcatcgacca cctgtctggc ctgtggtaca ccttcacgtg
     6241 ctgctgtcgc acagacccca ctgcgatcac gcgagacaat ctgtatttcc ggatattttc
     6301 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     6361 tatcaagatt gacgattaac gtaccgtaac ggtggccccg atgccacgcc cactcatcaa
     6421 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagga
     6481 gagtatcagc actgcaacgg agacccactg ccgttgctgc ccgttttccg gcctcacctc
     6541 cggcgattaa tcccagttga cgctgtaaat tttgtaacgc ctgtgaagac tgcagatgtg
     6601 aaccaccgaa gggaacaaac gactcggcaa aaatttttcg tgaacaagcc gggttacggc
     6661 tgtacctgtg gcggacggaa aatacaagcc atagcgtctg ccctgagcag ggtagatgct
     6721 gtatccggcg ccgacgacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     6781 gtgctgtttt gcgttttccg cggagatgga ggggtatacc gtcagagtta ataatttgtc
     6841 gactacatcg ccatccgcaa ggtaactgta gaagtgtttt aagagagtgc attgcgttac
     6901 ccataactaa aaacagctta gatccttgtt caccataaag tttcatccat tttctccacc
     6961 caggttgcgg aagaaccact tttgcaccgt tcgtgacagg agcaggattt ttacgccagt
     7021 agccagagcc agaagtgggc gcgagacatc acgtacttac gtacggatga aggctggctg
     7081 tatctgggag tggttaatgt cgccacgtat gtctgcataa gagacctcgg aacgttatgc
     7141 taccgcatgt catttgcaaa aatgacagat agatatactt caatttaaac cttaacaatt
     7201 agcatgggaa tgctgtcaat cctctgtatg caaaaatggc atctgaatcg ctcattattt
     7261 agccctctgt gtcaaagcgt aaagtgttcc cagagagcct gtacatagat tcgtgtaatt
     7321 tcctgatttt gatatgttca tcgacaggaa tttatccccc ggaaagatta agaaaacaaa
     7381 aaacgaacat ttattcaaca catccggaac ctcatcgggt atatatcagc gcttatattg
     7441 ctgacattga ttgatacagg gtatcggtga cgtttttttg ctccatttcc ctcaaaatgg
     7501 acgcaacttc ccctctgcgg ctctcagccg caatactgca ttcgggccag cggctcatgc
     7561 atcataacca gctctgccag acggtaaccc cgcttcagcc ccgtaaaccg catctgactt
     7621 tcgcacagga tgcatttcag cgggtcaacc ttcagtaacc gctgatacat ccctctccac
     7681 ctgatttgca tcgccgtttt tatcaccgtc tctgttatta cgtacactaa ttcttccagc
     7741 aaccggcgtt tcgccggact aaggaacccg tagaacctca ccatacggaa ccccttatcc
     7801 gccacatgcc aggagaacct ttccataaac gcatcaccac tcatcaatag gtattcttca
     7861 cgtcgtgtgc ggtgactgtt gtaacgcagt ccgatttcat cctgaccggc atagtgctcc
     7921 agacggctca ttggcaccgg cggctttttc aggtaagagc caaagtacac cgccacatgt
     7981 gtggcgttat ccatcacctt tgatacgttg acattcctgc cccgtcggta atgactgtcc
     8041 aggaaacgat tccattcccg tttactgctt ccttcagcag ccagctcatc cggtatcaat
     8101 agttccgggt atttccgtga cagtaaccgt gttatccggt atcgctacat actcatcacc
     8161 ttacgggcgt aaaaatgaag gtttttccag gtgtgatgtg atgtcacacc gccggcagtt
     8221 gtcgataaat ggataaatgg atatgtggat atgtggatga cactgctggt cccggcccca
     8281 tgtgtggatt accgtgaata tccccggctt cacatctgct gctatgcggc tcatctctgc
     8341 cagtaaccac cggttgtgga acaccaggga ccagtactgg cagggaagcg tgaatacaat
     8401 tgctgccacg ggccgtcggg gaccagactc agcagatact gtatccactg tgcacccgct
     8461 ttcaccccgc agtgtgggca ggagcgtttt ttacaccgaa aacagacctt ctttgtattg
     8521 caacagtccg ggcagtacat gccgccggcg ctgataccgg caagaatggt cgtaaactcc
     8581 cgctctgtac agcgggcgat ttccggatgc ccttcgtcat caacacgtac aaaccagaag
     8641 accagctttt tgtttccggc atccacaaag aacggaatat tcaggtctgc gcaacactca
     8701 acagtatcgt caaaattatc gaagcgcaga atctctactt cttcttcgtc aaaaaaatca
     8761 tcttcgtgaa gttttacaac atagcgggga agtttgcttc tttgaggtgc gagttttcgt
     8821 ttacggggtt tagctgaacg tgccatataa ctacctgaaa gacaatgaca ttgtttgttt
     8881 ttataacggt aatttcaggc catgacaagc cgcagccgct aggctgccta ctcgatcgat
     8941 aggaatttaa atcccaaaaa gattaaaaaa acacccaaaa acggatgttt attcaacact
     9001 gcctgcgtgc catgagtgca ccgccgtcaa ttaaatggat cttatcgatt gatactgcat
     9061 atcactgatg gttttttacc ccattcccct gaaaatggac gcaacttctc cgatgcggct
     9121 ctcagcc
//
LOCUS       DAZKGS010000169         9113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKGS010000169 DAZKGS010000000
VERSION     DAZKGS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1234
                     /locus_tag="ACHOQY_004056"
     CDS             <1..1234
                     /locus_tag="ACHOQY_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9680048.1"
                     /translation="VKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILS
                     LAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGK
                     DNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINY
                     TKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISR
                     GIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKD
                     FCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTE
                     LNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADK
                     ALREAKSEGGNKVIIHHI"
     gene            1415..2905
                     /gene="dgcJ"
                     /locus_tag="ACHOQY_004057"
     CDS             1415..2905
                     /gene="dgcJ"
                     /locus_tag="ACHOQY_004057"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9680049.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2948..3451
                     /gene="yeaK"
                     /locus_tag="ACHOQY_004058"
     CDS             2948..3451
                     /gene="yeaK"
                     /locus_tag="ACHOQY_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9680050.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3452..3556)
                     /gene="yoaI"
                     /locus_tag="ACHOQY_004059"
     CDS             complement(3452..3556)
                     /gene="yoaI"
                     /locus_tag="ACHOQY_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9680051.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3726..4172
                     /gene="yeaL"
                     /locus_tag="ACHOQY_004060"
     CDS             3726..4172
                     /gene="yeaL"
                     /locus_tag="ACHOQY_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9680052.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4129..4950)
                     /gene="nimR"
                     /locus_tag="ACHOQY_004061"
     CDS             complement(4129..4950)
                     /gene="nimR"
                     /locus_tag="ACHOQY_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9680053.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5047..6228
                     /gene="nimT"
                     /locus_tag="ACHOQY_004062"
     CDS             5047..6228
                     /gene="nimT"
                     /locus_tag="ACHOQY_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9680054.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6283..6630
                     /gene="yeaO"
                     /locus_tag="ACHOQY_004063"
     CDS             6283..6630
                     /gene="yeaO"
                     /locus_tag="ACHOQY_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9680055.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6652..6906)
                     /gene="yoaF"
                     /locus_tag="ACHOQY_004064"
     CDS             complement(6652..6906)
                     /gene="yoaF"
                     /locus_tag="ACHOQY_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9680056.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7089..8114
                     /gene="dgcP"
                     /locus_tag="ACHOQY_004065"
     CDS             7089..8114
                     /gene="dgcP"
                     /locus_tag="ACHOQY_004065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP9680057.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8147..8245
                     /gene="yoaK"
                     /locus_tag="ACHOQY_004066"
     CDS             8147..8245
                     /gene="yoaK"
                     /locus_tag="ACHOQY_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9680058.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8248..8322
                     /gene="yoaJ"
                     /locus_tag="ACHOQY_004067"
     CDS             8248..8322
                     /gene="yoaJ"
                     /locus_tag="ACHOQY_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9680059.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8381..8629)
                     /gene="yeaQ"
                     /locus_tag="ACHOQY_004068"
     CDS             complement(8381..8629)
                     /gene="yeaQ"
                     /locus_tag="ACHOQY_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9680060.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8857..>9113)
                     /locus_tag="ACHOQY_004069"
     CDS             complement(8857..>9113)
                     /locus_tag="ACHOQY_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9680061.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2256 a         2199 c         2131 g         2527 t
ORIGIN      
        1 tgtaaaactc atcccacaga tgaattacct gatggttgtt gtggctttgt ttttccttaa
       61 cgccgtcatt tttcttttca tgttaatgaa atatttcact aacaaacaaa ttttaccaac
      121 actcatttta agccttgcat ttttaagtgg ccttatctat ttagttgaaa ccattgtaat
      181 tatccataaa ccaattaacg gcagtacact gatccagaca aagtcgaatg atgtttctat
      241 tttctatatt ttccgccaac tcagttttat ttgtttaacc tcgctggcac tcttttgtta
      301 tggaaaagac aacatccttg acaacaataa gaaaaaaacg ggaatcctgt tgctggcgct
      361 gattcctttt ttagtttttc cccttctggc acacaatctg agcagttata acgctgacta
      421 ttctttgtat gtcgtcgact actgtccgga caaccatact gcgacctggg gaatcaacta
      481 tacaaaaata ttggtttgtc tgtgggcatt tttactgttc tttattatca tgcgtacacg
      541 attagccagc gaactatggc cgttaatagc attattatgt ctggcatcgc tatgctgcaa
      601 cttacttcta ctgactctgg atgagtataa ttacaccatc tggtatatca gtcgcgggat
      661 tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt tataacattt ttcaggaact
      721 gcaactctcc agcaaactgg cagttcatga tgtgctgacc aatatttata atcggcgcta
      781 ctttttcaac agcgtagagt cattattgtc gcgacctgtt gttaaggact tctgtgtcat
      841 gctggttgat attaatcagt tcaaacgcat caatgcccaa tggggacatc gtgtgggtga
      901 taaagtgctg gtttcaattg tcgatattat ccagcaaagc atccgccctg atgatatttt
      961 agcgcgactg gagggtgagg tgtttggctt gctatttacc gaactcaata gtgcccaggc
     1021 aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc ctgaccggct ttagtaacag
     1081 atatgatgtt cctgaacaaa tgaccatcag tattggcacg gttttttcaa cgggtgacac
     1141 gcgtaatatc tcgcttgtca tgacggaagc agataaagcc ttacgcgaag cgaaaagcga
     1201 ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa aaatattcag agccatgctt
     1261 tttgcgtggc ttttgcatac aatttattac tattacccct aaatttcccc tccccagcgg
     1321 cgcagagatg agtataatta gcgcccctgt gccaggccgc aatcgaactt tatctggttt
     1381 tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa ttgcaccata gaatgctccg
     1441 gcattttatc gccgcaagtg tcattgtgct gacatcttcc ttccttattt ttgaacttgt
     1501 cgccagcgac agagcaatga gtgcctatct gcgctatatc gtgcagaaag cagactcctc
     1561 ctttctttat gataagtatc agaatcagag tattgccgcg catgtgatgc gcgctctcgc
     1621 tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc
     1681 tgccaataat acccatggct taaacctgac tgcccataaa tacccgggct tacgcggcac
     1741 actacaaacc gcatccactg actgcgacac aattgtggaa gctgcagcac tattacccgc
     1801 ttttgatcag gcagtggaag gcaaccgcca ccaggatgat tacggttcag gtcttgggat
     1861 ggccgaagag aaatttcact attatctcga tctcaatgac cgctatgtct atttttatga
     1921 gccggttaat gttgaatact ttgcgatgaa taactggtcc ttcctgcagt caggaagtat
     1981 tggcatcgat cgcaaagata ttgaaaaggt atttaccggg cgtaccgtat tgtcgagcat
     2041 ttaccaggat cagcgtacta aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc
     2101 agggcagcta aaagggattg tgctgctgga tattaacaaa aacaatttgc ggaatatctt
     2161 ttacactcat gaccgccctc tcctctggcg ttttctcaat gtcacgctaa ccgataccga
     2221 ttcggggcgc gacattatca tcaaccagag cgaagataat ctgttccagt acgtcagtta
     2281 cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc attgatattc tttactttat
     2341 cacgtcttcg tggaaaagcg ttctgttctg gattttgacg gcgttaattt tgctgaatat
     2401 ggtccggatg cacttccgtt tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat
     2461 gactggactg tataatcgca aaattttaac ccctgaactg gagcagcggt tgcagaaact
     2521 ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac atggacaagt taaagcaaat
     2581 aaatgacacc ctcggtcatc aggaggggga tttagcgatt acgttattag ctcaggcgat
     2641 taaacaatcg attcgtaaaa gtgattatgc catccgactc ggtggcgatg aattctgcat
     2701 cattcttgtc gattcgacgc cgcaaattgc agcacaactg cctgaacgta tcgaaaaacg
     2761 tctgcaacat atcgcgccgc agaaagaaat cggcttctct tccggtattt acgcgatgaa
     2821 agaaaacgat acgttacatg atgcgtataa agcttccgat gagcgtttat atgtcaataa
     2881 gcagaacaaa aacagccgtt catgataacc ttctgtggtt gtttgcttgt aatctcagga
     2941 gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat cagcggttaa tcgctttatt
     3001 atcacaagaa ggtgctaact tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc
     3061 ggtgtcagaa attcgtggca ccgctctagg ccagggtgct aaggcactgg tatgtaaagt
     3121 caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc gctgccgatc aacaggccga
     3181 tctgagccaa ctcgccagcc atataggcgg attacgcgct tcgttggcca gcccggcgga
     3241 agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa
     3301 actcaaactg gttgccgacc ctctactctt cgaacgattt gatgaaatcg ccttcaatgc
     3361 agggatgctg gataaatccg ttattttgaa aaccacggat tatttgcgca ttgcgcaacc
     3421 agaactgctc aatttccgcc gcactgcgta actagcctgt ccgttcaata agcagaacgg
     3481 acaaaaccag cacaacagca atggcaaaaa acgatgacgt gataatcagt gtctcgacga
     3541 acatttgatc gttcatagca tgcccccggg agatagctgt tttcacgtta ttgttagcgt
     3601 gcacaaatgg cagtttgatg acagttcgcc tgatttttta tgcaaaaaac gtaaagattt
     3661 tctacttcct tcctgcagca agcgtaaagt aagcaggctt attatttttt ggcaaggaaa
     3721 ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt agctgcgctg ggctttatca
     3781 gtcataacac cactgtcgcc gtttcaattc tggtgttgat cattgtccgc gtcacaccgt
     3841 taagcacctt tttcccgtgg attgaaaaac aggggctaag tatcgggata atcattctga
     3901 cgattggcgt tatggccccc atcgccagcg ggacgctacc gccctcaacc ttgattcact
     3961 cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg
     4021 gtgggcgcgg cgtgacctta atgggcagcc agccgcaact ggtcgccggg ctgctggtag
     4081 gcactgtttt aggcgtggcg ctgtttcgcg gcgtaccggt cggcccgctt attgccgcgg
     4141 gtctggtttc gctgattgtg gggaaacagt agttaatccc gcaatatatc ggcctggcgt
     4201 ttgccccagg cctttcttaa acatagtaat aaaggccgtt gttgaatcat accccagcgt
     4261 atgagcaact ttctgtacag tatcgccttt cactaatccc tgaagtgcca taatcagctg
     4321 taactgctga cgccactggc gaaaactcaa ccccgtctct tttactatca gtcgcgcaag
     4381 attgcgttca ctcatcgcaa aaaagccagc ccattgccct aatgaccccc actcgacggg
     4441 ccctttcgcc atcatctcca ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg
     4501 cagctgttgt tgtggctgct gagggagttc atcaaacagg acctgaatta atctgcgggt
     4561 catcggctca gcacgctgag tcgtcgtcct gtttgccagg gttaaaatca attcccggca
     4621 taagggcgat attttcaatg tgcaacatgt tgtcggcatt gtcactgcgc tgggttcgat
     4681 aaacagaaaa cagagttcag cgttagctgt cacttgattg ctatgttcca ccccgcccgg
     4741 tatccaaacc gcatattgcg gcggcaccat ccacaaagca ttttccaccg tacaggtaat
     4801 tgcaccatgt aatgctagta tcagctgtcc tttgcggtgc tgatgtacag gactggttaa
     4861 ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc
     4921 atagccattg agattcaacc tgtgcatcat tttgtccgaa tttagcgata atttgtcatt
     4981 ttagcttgat tcaacataac aataaaaacg gtaaggtaca gcctcgtttg taacaatgag
     5041 aagcatatga cctgttcaac ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc
     5101 attctgatga ttgccactac attacgcgtc accttcaccg gcgcagcacc gttactcgat
     5161 acgattcgtt ccgcttactc gctgacgaca gcgcaaaccg gcttattaac caccctgcca
     5221 ttattggcct ttgcgctaat ctcacctttg gctgccccgg tggcgcgacg ttttggtatg
     5281 gaacgtagcc tgtttgccgc gttacttttg atctgtgcag gtatcgcaat tcgttctctc
     5341 ccttcgcctt acttattatt tggcggtaca gcggtcattg gcggtgggat tgcattaggc
     5401 aatgtcttac tgccaggatt aattaaacgc gatttccctc attccgtcgc cagacttacc
     5461 ggcgcatatt ccctgacaat gggagctgca gcggcactgg gatcggctat ggtcgtgccg
     5521 ctggctttga acggttttgg ctggcaaggc gcgttgttca tgctgatgtg ttttcctctg
     5581 ctggctcttt ttttatggct gccacagtgg cgaagccaac aacatgcaaa tttgagtacc
     5641 tcgcgcgcct tacatactcg gggtatctgg cgttcgccgc ttgcctggca ggtcacattg
     5701 tttcttggga tcaactcact ggtctattac gtgattattg gctggcttcc ggcgatcctc
     5761 atcagtcgcg gctatagcga agcacaggcg ggttcactgc atggtttgct gcaactagcc
     5821 acagcagctc ccggtttgct gatccctctt ttcttacatc atgtgaaaga tcagcgtggt
     5881 attgcagcgt tcgttgcctt gatgtgcgca gtgggcgctg ttgggctctg ctttatgcca
     5941 gcgcacgcga tcacctggac tctgcttttc ggttttggtt ccggcgcaac aatgatactg
     6001 gggttgacgt tcattggtct tcgggcaagt tctgcgcatc aggcggcggc actctcgggg
     6061 atggcacaat ccgtcgggta tttgctggca gcctgtgggc cgccgctgat gggtaaaata
     6121 cacgatgcta acggtgactg gtccgtacca ctgatgggtg ttgccatact ttcactactg
     6181 atggcgattt tcggactttg cgccgggaga gacaaagaaa ttcgctaata tccggtgcta
     6241 tagtgacgtg acaaatcatg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa
     6301 cgcgtttatg atccggctga acagagcgat ggttatcgcg tactggtcga ccgcctctgg
     6361 ccgcgcggta tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg
     6421 tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag
     6481 caatatcttg cagaactggc gcaacacgag caagaaggaa agcggctggc ggacatcgcc
     6541 aaaaaacagc cgctgaccct gctctacgca gcaaaaaaca ccacgcagaa ccatgcgctg
     6601 gtgctggccg actggctacg tagcttgtga ttttagtaca gcatccggcg gttatttttc
     6661 accagccgga tggtcccgtc gccacaatgc ccattcatca atcgtttcac cgcccggtaa
     6721 tttgcaattg ttgctgaccc cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg
     6781 ctggcaatag accgacgccg gatttgccat accaatctgc ggcggtttag gtgcttctgg
     6841 ctgagaagag gttgaacaac cagccaggac cagcaagcaa ggcaaagcaa aactgataat
     6901 tttcatttat tgatctcaca catttatcta cgcttagagt atcgcggtat cgttttgttt
     6961 tgcagcacta tttttattac attcactcaa aacatattac gtcttgtttc atctttgttg
     7021 atgatgtttt atcatgcctg caaagattaa ataatcagca tttacccgcc gtatcctgga
     7081 gttgtttcgt gtcagatcag attatcgccc gcgtctcgca atcccttgcc aaagaacagt
     7141 cactagaaag tctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat
     7201 caacctacct gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg tttgcccgta
     7261 acagccagaa aatgcacatc ccggagaatt ttaccgtctc gtgggattac tcgttatgca
     7321 aacgcgctat tgatgaaaac tgctttttca gcgatgaagt ccctgaccgt tggggcgact
     7381 gtattgcggc acgcaatctt ggaatcacca catttctgag tacaccaatt cacttaccgg
     7441 atggatcatt ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac
     7501 gcgcggaaca ggttttacag ttattcgccg gactgattgc acaatatatt caaaaagagg
     7561 cgttggttga acagctgcgc gaagccaatg ccgcgctgat tgcgcaatcg tataccgact
     7621 cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa tctgacgacg ctgttttctc
     7681 tcgcccggca tcttaaccat aagataatga tcgcgtttat cgatctggat aacttcaaat
     7741 taatcaatga tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc
     7801 gccttaatac gctccagcaa aatggcgaag ttattggtcg tctcggcggt gatgagtttt
     7861 tggttgtttc actaaataac gagaatgcgg atatttcgtc gctgcgagaa cgtattcaac
     7921 agcaaatacg tggagaatat cacttaggtg atgttgattt gtattatcct ggtgccagtc
     7981 ttggcattgt agaagtcgat cctgaaacga ccgatgcaga cagtgccctg catgctgccg
     8041 atatcgcgat gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcattcag
     8101 cgccacattc ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat
     8161 tatttttccg gttgtaatct ttattacagc ggtcgtattt ttagcatggt tttttattgg
     8221 cggctatgct gccccgggag cataaagatg aaaaaaacaa cgattattat gatgggtgtg
     8281 gcgattattg tcgtactcgg cactgagctg ggatggtggt aacgtcacct ctaaaaaata
     8341 gcaaaggctg cctgtgtgca gcctttgtgc aatttaagcg ttaactttta atcttcctgt
     8401 agataaatag cacgacaatc gcaccaataa cggcaaccac gaagctgcca aaattgaagc
     8461 catcgacttt accaaagcca aacagcgtgc tgatccatcc gccaactacg gcaccgacta
     8521 tccccagcag gatagtcata aagaatccac caccatcttt acctggcatg atccacttcg
     8581 ccagaatacc ggcaataagc ccaaaaataa tccatgacag aatgcccatt gtttcctcac
     8641 ttatctgttt tgcattagcg ggttagtcgc tgataaaaag catagcacaa catcgggagg
     8701 gcaagatttg tgacgagcat cacggaggat ttttttgcgg atggcacaga aattgcgcca
     8761 tcaacgatca gtgataatta ccaaccacaa acatcatgtt cgttttccgt gtcttgacat
     8821 cctccacgcc ctgaatgacg aggatccctg ctacgttcag gctgtcgcct gaatcatttc
     8881 ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg caagaacggc
     8941 gtgccccgcc gctaaaatgt tacgagcgcc gtttacatcg gcgttcgctg tatatccaca
     9001 tacctggcat ctgaattgac tttgtgacag gcgattttct ttcgctgtat gaccacagca
     9061 cgcgcaacgc tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cac
//
LOCUS       DAZKGS010000170         9023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKGS010000170 DAZKGS010000000
VERSION     DAZKGS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..271
                     /locus_tag="ACHOQY_004070"
     CDS             <1..271
                     /locus_tag="ACHOQY_004070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680062.1"
                     /translation="KINIKNSAKFNIRSTIRLTYDIIRQLHLPQNGYFMERSNFLVAI
                     IRLILRSLAIIPGWFVSLVHEIVTFLFILEIWGKAGDDFFICIRW"
     gene            1149..1865
                     /gene="nanC"
                     /locus_tag="ACHOQY_004071"
     CDS             1149..1865
                     /gene="nanC"
                     /locus_tag="ACHOQY_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9680063.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1885..2991
                     /gene="nanM"
                     /locus_tag="ACHOQY_004072"
     CDS             1885..2991
                     /gene="nanM"
                     /locus_tag="ACHOQY_004072"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9680064.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3056..4036
                     /gene="nanS"
                     /locus_tag="ACHOQY_004073"
     CDS             3056..4036
                     /gene="nanS"
                     /locus_tag="ACHOQY_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9680065.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(4044..4694)
                     /locus_tag="ACHOQY_004074"
     CDS             complement(4044..4694)
                     /locus_tag="ACHOQY_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9680066.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            4831..4974
                     /locus_tag="ACHOQY_004075"
                     /pseudo
     CDS             4831..4974
                     /locus_tag="ACHOQY_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(5638..5766)
                     /locus_tag="ACHOQY_004076"
                     /pseudo
     CDS             complement(5638..5766)
                     /locus_tag="ACHOQY_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5742..5891)
                     /locus_tag="ACHOQY_004077"
                     /pseudo
     CDS             complement(5742..5891)
                     /locus_tag="ACHOQY_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(5976..6173)
                     /locus_tag="ACHOQY_004078"
     CDS             complement(5976..6173)
                     /locus_tag="ACHOQY_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9680067.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(6185..6673)
                     /locus_tag="ACHOQY_004079"
     CDS             complement(6185..6673)
                     /locus_tag="ACHOQY_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9680068.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(6670..7044)
                     /locus_tag="ACHOQY_004080"
     CDS             complement(6670..7044)
                     /locus_tag="ACHOQY_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9680069.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(7134..7502)
                     /locus_tag="ACHOQY_004081"
     CDS             complement(7134..7502)
                     /locus_tag="ACHOQY_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9680070.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPMPETKK"
     gene            complement(7665..7886)
                     /locus_tag="ACHOQY_004082"
     CDS             complement(7665..7886)
                     /locus_tag="ACHOQY_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9680071.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(7949..8425)
                     /locus_tag="ACHOQY_004083"
     CDS             complement(7949..8425)
                     /locus_tag="ACHOQY_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9680072.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(8441..8926)
                     /locus_tag="ACHOQY_004084"
     CDS             complement(8441..8926)
                     /locus_tag="ACHOQY_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9680073.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT         2479 a         2032 c         2198 g         2314 t
ORIGIN      
        1 taaaatcaac ataaaaaatt cagcaaaatt taatattcgt tcaactatcc gtctcacata
       61 tgacattatt agacaactcc atttgccaca aaatggctat ttcatggaac gcagtaattt
      121 cttagtagct attatcaggc taattcttag gtccctagcg attattcctg gctggttcgt
      181 cagtttagtt catgagattg ttacattcct ttttatcctg gagatatggg ggaaagcagg
      241 agatgatttt ttcatctgca tccgttggta aatatggctt tggtcactgc gacttaaacc
      301 ttcctcaatc acagctgttc gccgggctat tcggcaaaca agctaagtga aacatcatcc
      361 tgaaaacacc aacatcaaca agcctctcca gagagacttc aggagtgacc agctacaagc
      421 cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
      481 cttattttca ccacgcccac tgagttatct tgcaaatctt tccttgttta tactctaaat
      541 aaattactta taaaacagat aattaaataa caacccttgg tttgtgaagg cctccgcaag
      601 tgttcgtttg gcttcacatg gcatcttctt cttagaaaaa tatcgacata ttttgtgaca
      661 cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg tataacaggt
      721 ataaaggtgc accaagatag tcaatgagac agggcatctc gcaatctatg gcaaacatca
      781 cttcagttct ttcacatcgg gtgatgaaaa tgcacttcag tctgaaagga atatgaaaat
      841 gagatcaaca gacactctat tttatgactc tgggtaaaat ggattgagta agtgatatag
      901 cttacgaaca ttcaaatcaa ttaaacatca gaagagattt tatactcagg tatttaatct
      961 ggctctctgt ttatttaaat aatgtgaaaa gagatttttc acaggagacc ttatacaaaa
     1021 aatataaaat acagctaccg gttgccaaag acactataag cctggcaaaa aaatattaca
     1081 caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtttcc tacaaatgag
     1141 tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc ttttcgtcac
     1201 cattaatttc tcaagctgcg acactggacg tacgtggtgg atatcgtagt ggaagccacg
     1261 cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg gcaagtatgg
     1321 aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca ctcaatgatg
     1381 ttcaggttga tgttaattac gcgattaaac ttgatgatca atggacggtg cgcccgggaa
     1441 tgttaacgca ttttagcagc aacggcacac gctacggacc ctacgtaaaa ctgtcctggg
     1501 acgcgacaaa agatcttaat tttggcattc gctatcgtta cgactggaaa gcttaccgac
     1561 aacaagactt atccggtgat atgtctcgtg ataacgttca tcgttgggat ggatatgtca
     1621 cttaccatat taatagtgat ttcaccttcg catggcaaac gacgctatac agcaaacaga
     1681 acgattatcg ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat
     1741 accatatgac gccggatatt acgccataca tagaatatga ctaccttgac cgtcagggtg
     1801 tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac
     1861 tgtagtagac aggagacagt cacaatgaat aaaacaataa cggcgcttgc tatcatgatg
     1921 gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt taaaagtggt
     1981 accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg tacggcatgg
     2041 tacaagctgg atacacaggc caaagataaa aaatggaaag cgttagctgc attccctggt
     2101 ggaccaagag atcaagcaac ctcggcattt attgatggca atctgtatgt gtttggcggc
     2161 attggcaaaa acagcgaggg cttgactcag gtatttaatg acgtacacaa atacaacccc
     2221 aaaaccaata gttgggttaa attgatgtcg cacgcaccga tgggcatggc gggtcatgta
     2281 acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat
     2341 ggctattttg aagatctcaa cgaggctgga aaagattcaa ccgctataga taaaatcaac
     2401 gctcactatt ttgacaaaaa agcagaagat tattttttca ataagtttct gttgtctttt
     2461 gatccctcaa cacagcaatg gagttacgct ggcgaatcgc cctggtacgg aacggctggt
     2521 gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc caaaccagga
     2581 ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta ataatttaaa atggaataag
     2641 cttgctcccg tctcatcacc agatggcgta gctggcggtt ttgcggggat aagcaatgat
     2701 tctcttatat ttgccggagg ggccggattc aaaggttcac gagaaaatta ccagaacggt
     2761 aagaactatg cgcatgaagg cctgaaaaaa tcatatagca ctgatattca tctttggcat
     2821 aacgggaaat gggataaatc gggtgaatta tcgcaaggtc gggcctacgg agtatcattg
     2881 ccttggaata atagtctatt gattattggc ggtgaaactg caggcggcaa agcggtgacg
     2941 gattcagttt tgatctctgt gaaggataat aaagttacag tacaaaacta acgcttcagg
     3001 gccccggtaa aggggccctg ctatcaattt gtcatttcaa ttacgcgaaa tttatatgaa
     3061 cgcaataata tcgcccgatt attactatat tcttaccatt gctggtcagt ctaatgccat
     3121 ggcgtatggc gaaggactgc cattaccgga cagggaagat gcgcctcatc ccagaatcaa
     3181 acaattagcg agatttgcgc atacgcatcc cggaggcccg ccatgtcact ttaacgacat
     3241 gattccactg actcactgcc cacacgatgt tcaggatatg cagggttatc accatcctct
     3301 ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata tagcacggaa
     3361 attactgccc tttattcctg ataatgcagg ggttctcatc gttccgtgtt gccgtggcgg
     3421 atcggctttt accgcgggca gcgaagggac atattcagaa cggcacggag ccagccatga
     3481 tgcttgtcgt tggggaacgg atactccgct ataccaggat ttagtcagca gaacgcgagc
     3541 cgcactggca aaaaatccgc ataacaaatt cctcggcgta tgctggatgc aaggcgaatt
     3601 tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata tggttgaagc
     3661 ctttcgtagg gatctaaaac aataccattc tcagcttaat aatattactg acgcaccgtg
     3721 gttttgcggc gataccacct ggtactggaa agaaaatttc cctcatgcgt atgaagttat
     3781 ttatggcaat tatcaaaata atgttttagc caatattatt ttcgtcgact tccagcaaca
     3841 aggtgaaaga ggactgacga acgcgcctga tgaagacccg gacgatttaa gcacgggata
     3901 ttacggttca gcgtaccggt caccggagaa ctggacgacg gcactgcgaa gcagtcattt
     3961 cagcacggca gcccgtcggg ggattatttc tgacaagttt gtagaagcaa ttttgcagtt
     4021 ttggcgcgaa aggtgagcgt tcattaattt gatttattcg catccatcag cgcttttaac
     4081 tgctccggcg taaacggtgg tttgcgtcgg tgcaacatcg catggcaatt agggtacaca
     4141 ggcagcaaat cattaactgg atccagctgg taatcctctt taatggctga cagcggcacc
     4201 aggtgatgca catggataaa gccttcagcg atatcaccat agatcttcac cagatcaaca
     4261 ccgcatacgt tgcactggca tccatgatgt tcgaccgctt tatctcgggc ctttttatcg
     4321 cgttcatagc ggtttactgt cacctttatc gccgcacctt caaggtatgt ctcctgcgac
     4381 ggcaactcat ccgggaacag aacgtcggct atgggagccg cagcggcttt atccagatga
     4441 atatgaaatc cctgctctat gagtagcttg acacatttag attttattcc gccggtgaaa
     4501 tccgcagggg taaattgcgt ccccgtcatc aatgtcgctg cgatgccagc aatcgccttc
     4561 gaggggtaac gtcggccatg atattcaatc tcatacaacc gcgcaggctg gaactgatga
     4621 gtttctcccg catcccataa atggatagct tcgatgatat accgccgcgg aagatgatca
     4681 gggagtttat tcatggagaa tgtccttata cgttctcaga acaggcagca aaaaaacaaa
     4741 tgagatcaag gtaatatatc ttcacttgca ggcttgaaca gcagaaaatt tgcgagcatg
     4801 atcgcaatta tttcactgcc agggatatca atagcttcca gtaaatcgct acagcaggca
     4861 attgccaata taaaaatctg gcacaagggt gaacagcgcg cgccgcataa gccattgtta
     4921 ttgctatacg tattagcggg atacctgaat ggacatccgc gccttttcga ttattgtagt
     4981 ggaacagtct acgaggatgc agttcgaagt gagtaagtaa aggttgcggg aaacgatcaa
     5041 tgccgccata caatgatttt ttaggatcaa caatgagtaa taaaaactac gaaagtcacc
     5101 gcaaagcgat tgttagtaaa ggtataccac ccgctctatt aaataggctc accaattcgg
     5161 atgttcaggt gatcaatacc ttcttaacac gagtgagcaa actggaacta tctcaacaag
     5221 agaaagactg gatcataaaa atcatctcta tggtttagac aatttcagcc ttgtagcctt
     5281 tcaagggttt tgtgtgggta caaacattat cgaatcgggg tatagccgca gctgtagctg
     5341 tatccacatc agtaagttga tgtaggttga cttaggttga gcgaaaaata gcgaaaagcc
     5401 ttgtgtggcg cggatttcag tcataaaaaa acgtccgttg atgtctattg atgttccgat
     5461 ggtgcgaagg tgcgaaggtg cgaaggccgg actcaaacat gtgacttaac ctcatgattt
     5521 aaaattgtta ataataaaca atatcatctt ataccaacac agataccaac acgaaaaatc
     5581 actggttttc ggcatcgaac caggacaatc agctcctgat tgagataccg agctctgtta
     5641 ctcgtcatct tcctcgacag gcttcattgc atcaagtagt actcgcacca tcgcgataga
     5701 accaatccgg ataaaagcat cttctccatc tatatctgtt aactgatatc gctcacttga
     5761 tgccggataa ttgtgtcgat ttgcagcagt gaactctttc gtacagccag aatgcggcgg
     5821 atgctggttg agcagataac gtccgtattc tcgttgacca cttccggacg gtaggctgtg
     5881 atatcagaca taatcgatat tcctttttgc agataaatga aaatgccagc cctgttcagg
     5941 ctggcgtcag tggaatgaag tctggtgagt aagactcacc ggttctggtg ctgaagctgc
     6001 agttggcgca gggtacggat atcctctctg gcctgttcga tacagggcaa cagtgctgct
     6061 gaatccgttt tatcctcatt gttgtcaaag ataattcccg attcgcagtc gatattgtcc
     6121 tgcagccacg caatcagaat atccagtgcg gtttccgtgg ttaatgattt catgttgttc
     6181 ctggttacca gttgaaagtg ggtaaacccg gcaggcattc agcgctggca tccagatgaa
     6241 tgagactgac gccataacgt cgggtaagtg tgataaccag acgacggaac gtttttgaca
     6301 gccccaggcg tttaagccgc agaaccggat atgaccacgc atcggtacgt aacagatatc
     6361 cgctacctgt gaaatgaatc cattctgatt caccaacgtc actggtctga tgcgacagcg
     6421 agtacagcca ggcgttatcc ttttccgtga tatgtgcggt actgcagcgt atgccggcaa
     6481 gggttgcaaa cggtggtagt gtgcaggttg actgttggtc ggattcctcc accacgcgga
     6541 gtgaataacc gttttcagcg accttgttaa tcagttcagt gagattaaca ccatcgacgt
     6601 caacgacaat tcgccccatg ttcagtgcct gcacattaat tctgtcggct tccagcatca
     6661 gggaaagttt catcgtttct ccggatgctt acctagggta atgttattta ccgttctgta
     6721 attgtcgcgg gtcatcaggc cggtcgccct gcgagcccgg aggatatcaa tactgtttat
     6781 taactgagag cggggacaag tgctgaatcc cggctggtcg gtacgcacca gcgcgtattt
     6841 ttccacgaga aaattcaccg catcacacag tgaaatgcca gcttcaatat gctgctcaat
     6901 cacacgttca tcggcgaacg gtgtgtcatt cagtgtaagg ccgtaatgct ggcccagtag
     6961 tcgggagagc agagtctgcc agatggtgac gggagacggg cagctcgatg cctcccgtac
     7021 gtgtgtatcg ggtaatgttt tcatactgaa ggttttcctg atatgcagag atgaaaaatg
     7081 gaaaagtggc gtggtgaaag caccaggccg gagcagaagg ttattccggt ggtttacttt
     7141 ttcgtttcgg gcatcggata aacagccaga taaacgtaac caccgctacc gagagtgtca
     7201 gctttgcagg tcagaccttt tgcatacagc gtgacggtat gctgatggcg gggattcagt
     7261 tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gcggaaaggc ctggtccagg
     7321 tggtacgcat ctgcatcgct gaacaggcct ctgataccgg cgcggtcggc aaggtaatgc
     7381 aggcggttac cctcctgcac cagacgggcg ccgaaacagg gcgtcactgt gcagggtagt
     7441 ccccaccagg gatggtcgtc attgtcatcg ggatgcgttg tcccggaaag tgtgtctgac
     7501 acgataaaat cctcacagaa aatcggtgaa tgatagttta acgatgacgg gtcagccggc
     7561 gcgcagaccg gcttcggtaa aggtgtattc gttgatgagc aggacctcat ccacagcggc
     7621 gtctgagctc agccagtcgt actcgttttc cgggtgacgg aacatcagtt cagggtgacg
     7681 ctcatcagcc ggacatacgg accaaaactg tccttacggc gttctgcaaa cacggccagc
     7741 acaccgggaa tatcctgcac ttcacgaccg gtatacgctt cagcactgcc gtgccagcgg
     7801 tatttgccgg tacagaacgg aaaaagacgg gatgccggat gctgacggtg aatacgcatg
     7861 gcttcgccac gggtgatgat tttcatagtg ggatacctct gaagacagaa gataaaagtg
     7921 aaaacaggtg tgatgtggtt gtgacgggtt aaagcagacc atgttcggca aaggagaaaa
     7981 cctggctgcc accgactatc agatggtccg gcacccggat atccaccagg cccagtgcct
     8041 gtaccagacg ttccgtgata aggcggtctg ccttgctggg tgtgacttca ccggacgggt
     8101 gattgtgtgc cagcaccaca gcagcggcat tgtgatacag ggcgcgttta atcacttcac
     8161 ggggatggac ttccgtgcgg ttgatggtgc cggtgaagag ggtttcaccg gcaatcagct
     8221 gattctggtt gttcagatac agcacccgga actcttcacg ctccagtccc gccatgttca
     8281 gaatcagcca ttctcgtgcc gcacgggtgg aggtgaaggc cacgccgggt tcatgaagat
     8341 ggcggtccag ggttttcagg gcccgttgaa tgaggctgcg ctcgccgggc gtcatctctc
     8401 cgggcagaaa ggaaagctgt tgcattgtac ttctctccat tcagtcgatg atgcgcataa
     8461 tggcgctgca ttccggatgc tgcagggcgt aatcccgcag ccggtaataa tggaccgtca
     8521 tggcataaca ctccgtacga caggcatgat gactgtacgt catcagacag gcagcaatgc
     8581 cggcagcttc cggactcatt tcagcgcggt taccgttcat ggcattgaac agtacccatg
     8641 tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt
     8701 acagattcca gataccaccg cagtagtctt cacacagacg gtccatccag ccgaagacac
     8761 ggggctccag ggtcacccac tgtggaatga ggccaaagtg ctgcggccag aagctgatgc
     8821 gctgttcatc agggacgagg gtggcaacca actgaggctg gtcattccct gatgcagcgg
     8881 ttacggaaac agaagatgtg gtggaattat gtgaaatggt tttcatgaaa tgactcctta
     8941 aaggacaaat aaatgcgtgg ttttcagggt aatggctgaa tcagcgcagg ctctccagca
     9001 gggtttccgc catcacccac aat
//
LOCUS       DAZKGS010000171         8841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKGS010000171 DAZKGS010000000
VERSION     DAZKGS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(434..1663)
                     /locus_tag="ACHOQY_004085"
     CDS             complement(434..1663)
                     /locus_tag="ACHOQY_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9680074.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLSDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTALPAARRK"
     gene            1912..3033
                     /gene="roxA"
                     /locus_tag="ACHOQY_004086"
                     /gene_synonym="ycfD"
     CDS             1912..3033
                     /gene="roxA"
                     /locus_tag="ACHOQY_004086"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9680075.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(3179..4408)
                     /gene="pepT"
                     /locus_tag="ACHOQY_004087"
     CDS             complement(3179..4408)
                     /gene="pepT"
                     /locus_tag="ACHOQY_004087"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9680076.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            4658..5794
                     /gene="potA"
                     /locus_tag="ACHOQY_004088"
     CDS             4658..5794
                     /gene="potA"
                     /locus_tag="ACHOQY_004088"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9680077.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5778..6641
                     /gene="potB"
                     /locus_tag="ACHOQY_004089"
     CDS             5778..6641
                     /gene="potB"
                     /locus_tag="ACHOQY_004089"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9680078.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(6874..7023)
                     /locus_tag="ACHOQY_004090"
                     /pseudo
     CDS             complement(6874..7023)
                     /locus_tag="ACHOQY_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            7147..7232
                     /locus_tag="ACHOQY_004091"
                     /pseudo
     CDS             7147..7232
                     /locus_tag="ACHOQY_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(7255..7845)
                     /gene="espM2"
                     /locus_tag="ACHOQY_004092"
     CDS             complement(7255..7845)
                     /gene="espM2"
                     /locus_tag="ACHOQY_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFP9680079.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            8029..8679
                     /locus_tag="ACHOQY_004093"
     CDS             8029..8679
                     /locus_tag="ACHOQY_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9680080.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            8754..>8841
                     /locus_tag="ACHOQY_004094"
     CDS             8754..>8841
                     /locus_tag="ACHOQY_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680081.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTN"
BASE COUNT         2243 a         2162 c         1989 g         2447 t
ORIGIN      
        1 cacgtacggc taaggccggt tagtttttcg cattcttttt cacgtataaa ccgatcagtg
       61 cttaacacta ttgcccccct ttcgtttctt aaagagtcat caggtgtctt attgtgtcgt
      121 attgttcccg ctagagtgat gaatggcaaa tattgaggat gtatgattta cagaaaatga
      181 aacagtaaga ataaaatctt ttaaattcat gttaatacaa aggcataaaa tattgtttca
      241 tgcctttttt ctcacgcgtt aaagcgttat tcgctaatgt ataaaaaaac agtcacacaa
      301 ataaaatcag tcacttataa acctgtgtac tttttcgcct cttgttcgtg gttgttccac
      361 gttgtttcac attgttcgcc gttgtatctg tgtgcttatc cagtatgcgc atactgaaaa
      421 aacacgaaaa aaattatttt cttctggctg ctggtagtgc ggttacgttt tcatgtgttc
      481 ccgccagtat ccctaaccgc tccatccaca tatccagcgc attgcgttta gcatccagat
      541 aacgggaatg attataaact cgctgcattc ctggcatctg gtggcctgta agctgctcca
      601 cgacgtgagg atccacgcct aaatcgctca gcatcgttgt aaaggtgcgc cggatgtcat
      661 gtagtgacca gtgagggtga tttagcctcc tgtgcgctaa tctgccgtac tgcgacacgc
      721 ttgtttcctg tttcacttcc cccagcaata agcccgtgtg cctgttctgc tccaccagct
      781 gcgtgacgaa cggcagtatt gcttccggta tgggccggaa tattgccacc ttcgttttgc
      841 tgtgttcttt cggcacggtc cagagcattt cggtaaaatc ccactcgctg atctccgata
      901 acctcagttc taccgtccgg catccgaaca caatcaggag gcggattaac gcgatgtagt
      961 agggggagaa tatttttttg tccagtgcct gcaataattc gcccagttct ttggtgctta
     1021 agacacgctc gcttatatcc ggttttttcc caacgtccgc cacactcata tcatcaagaa
     1081 cgttgctgat tgcatagcgc cgcctccggc agaacttaag cgcctgtttg cacgtctgta
     1141 gcaagaatcc ggcagtaaca ggcgttcgct ttgccacctg gtcaaaacag gccagccagt
     1201 gccgtagctc gcatttatcc agcggcatag caccaatgtg ctgtattacg tgattattaa
     1261 ggcgcttttt cagggcggca taatccacgc ggttttcctt tgcgtacgac tcaagccagt
     1321 aggtgagcgc atcgccaacc gttaccggct ttaacgcttc ctgtacggtg taattaagct
     1381 catgacgtgg atttttcccc tctgccagcc atgcgcgaca ctgggcggct ttttccctgg
     1441 ctgatttcag gctcagatca ggataatttc ccagcttaat gcgttccggt ggtgcccccc
     1501 ttcccgttcc ggccctgtaa gtgaaatacc aggttaaaag gccactggtt gaatgcctga
     1561 cgctcaggtt tccaccgtca ttaagaaagg ctgttttttg ggcgggtgtg ccgttgattt
     1621 tcctcagctg tgtatcgctc agtttgttaa gtgctctgct cataattttg attccggtca
     1681 tcacaattaa ctacaccatt aactacaccg atcgttgcac aaagggctac aacgtgaaac
     1741 aagctggaac aaggaaaatc acaaatctgt ttataatcaa aaacataatg aacaatctga
     1801 aacattatga aacggcaaaa aacactaatt gataaaatat gtccatactt cacgcattac
     1861 gttaagcatc cgttataatc ggttgcagat accagcctgt ggatgcttaa catggaatac
     1921 caactcactc ttaactggcc cgattttctt gaacgtcact ggcagaaacg cccggtggtg
     1981 ttaaaacgcg gctttaataa ttttattgac ccgatctctc cagacgagtt ggcgggtctg
     2041 gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc aggatggcaa atggcaggtc
     2101 agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa ccaactggtc attactggta
     2161 caggcagtga accactggca tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta
     2221 ccggactggc gtattgatga tctgatgatc tctttttctg taccgggcgg cggcgtcggc
     2281 ccgcatctcg atcagtacga cgtgtttatc attcagggta ccggtcgtcg tcgctggcga
     2341 gtgggcgaaa agctgcaaat gaaacagcac tgtccgcacc cggatctgtt acaggtcgat
     2401 ccgttcgaag ccatcatcga tgaagagctg gagcctggcg atattcttta tattccgcca
     2461 ggattcccgc atgaaggcta cgcgctggaa aatgcgatga actattccgt gggttttcgc
     2521 gcgccaaata cgcgggaatt aattagcgga tttgccgatt atgtgctgca acgtgaactg
     2581 ggcggcaact actacagcga tccggatgtt ccacctcgcg ctcatcctgc ggacgttctg
     2641 ccgcaagaga tggataaact gcgtgagatg atgctcgaat tgatcaacca gccggaacac
     2701 tttaagcaat ggtttggcga gtttatatcc cagtcacgtc atgaactgga tatcgcgccg
     2761 ccggaaccgc cttatcagcc agatgaaatc tacgatgcgc tgaaacaagg tgaagtgctg
     2821 gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg acgtgtatgc caatggtgag
     2881 aagatcgatt ccccgcaccg tccggcactg gatgcactcg ccagcaacat tgcgctgact
     2941 gcggagaatt ttggcgatgc gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg
     3001 gtcaatagcg ggtattggtt cttcgaaggg taagtttgga tttaagccgg atgcggcgct
     3061 gattgcgcct tatcgtgcct acatgcactg aacgcctgta ggcacgataa gtatgtggac
     3121 cagtatcaat ataacggttt catcccggta attggcagtc cacatacgca tcgggcaatc
     3181 acgacttccg ttgcgccgtt aactcggcaa tacggacaat cacctgcacc gctttttcca
     3241 taccttccag agtcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaggt
     3301 tcgggcacgg taatcccata aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt
     3361 tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg ggcgatatcg ataatatgcg
     3421 gatgctcaac cactttctcg cgcatattgt agtaactgtc ttcaatcacc agttcaatgt
     3481 agcaatcagg atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacgtttac
     3541 gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gctcggtcaa
     3601 cggtgccttt catgctcgcc aggtgataga aaccttcata gccttctgtc atttccgggc
     3661 tttcatccgc cggaacttcc gcatgaatac gtgccgccag cgacagcgca tttaccatca
     3721 ctcctttcgc cgtgcccgga tgaacattgt taccgacaat tttgatattg accgatgcgg
     3781 cgttgaagtt ttcaaactcc agttcgccta cgccaccacc gtcaacagtg taagcccagc
     3841 gggcatcgaa ggcatcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg
     3901 taaaggcgac gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg
     3961 tcatgatttc tgcaatacct gctttgtcat cggcacctaa caaggtttta ccatcggtgg
     4021 taatcaacgt ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt
     4081 catcgccgat acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattca
     4141 catttttgcc gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga
     4201 tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca
     4261 cattgataag ccccatctct tcgagctgct ctttcagcag atgcaataac ttccattggc
     4321 cttccgtgct gggaacctgt ctcacccctg cttttgattg ggtatccaga gacacgtagt
     4381 tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc actttttgtg acaacattat
     4441 taagaagcaa gaaaagacaa atattgcgtc aggtcacttt tacccctgca agcgggaata
     4501 tttatcagca ttaccttaat gaatataaag ctaagccagt aattcacaac aaagattggc
     4561 gattcaagcg gtttgccgta gaattaccgc cctcattaag agtcaccaag gtggttaacc
     4621 acaaaccccg catcggtaag ccatccgttg cgtttacatg ggacagagta aaaaattgaa
     4681 taaacaaccg agttcgcttt caccgctggt gcaattggcg ggaattcgca aatgctttga
     4741 tggtaaagag gtcattcccc agctggatct gactatcaac aatggcgagt tcctcacgct
     4801 gcttggccct tctggctgcg gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac
     4861 tgttgattcc ggacgcatca tgctggataa cgaggacatc acccacgttc cggcggaaaa
     4921 ccgctatgtg aacactgttt tccaaagcta cgcacttttc ccccacatga ccgtgttcga
     4981 aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt
     5041 gatggaagcc ctgcggatgg tgcaactgga aaccttcgct caacgtaaac cgcatcagct
     5101 ctctggtggt caacagcaac gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct
     5161 gttgttgctg gatgagtcgc tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa
     5221 cgagctgaaa gcgttacagc gtaagcttgg tattaccttc gtctttgtca ctcacgatca
     5281 ggaagaagcg ctcaccatgt cagacaggat tgtggtgatg cgcgagggtc gtattgagca
     5341 agatggtaca ccgcgcgaaa tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat
     5401 tggcgaaatc aatatgttta acgccactgt catcgaacgt ctagacgagc agcgcgtacg
     5461 cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac ttcgccgttg aaccggggca
     5521 aaaactgcat gttctgctgc gcccggaaga cttacgtgtt gaagagatta acgacgacaa
     5581 ccacgctgaa gggctgattg gttacgttcg tgagcgtaac tacaaaggca tgacgctgga
     5641 gtcggttgtt gaactggaaa atggcaagat ggtgatggtc agcgaattct tcaatgaaga
     5701 cgatcctgac tttgaccact ctctcgacca aaaaatggcc attaattggg tagaaagctg
     5761 ggaggtcgta ctggctgatg aagaacacaa gtaagttcca gaatgtagtg attgtcacta
     5821 ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct gatgatcatt ggcaccagct
     5881 ttttgacccg cgacgacgcc agtttcgtaa aaatggtctt tacgctggat aactacacgc
     5941 gtctgctcga tccgctctat tttgaagtgc tattgcactc gctgaatatg gcgctgatcg
     6001 ccaccctcgc ctgcctggtg ctgggctacc cgtttgcctg gtttctggcg aagctgccac
     6061 acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc cttctggacc aactcattga
     6121 ttcgtatcta cgggctgaaa attttcctca gcaccaaagg ctatctcaac gagtttttgc
     6181 tctggctggg cgttatcgac acaccaatcc gtatcatgtt cacgcccagt gcggtgatta
     6241 tcggtctggt ttacattctg ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa
     6301 aactggataa gccgttgctt gaggcggcgc gcgatctcgg tgccagcaag ttacagactt
     6361 ttatccgtat cattattcca ctgacgatgc cgggaattat tgccggatgt ctgctggtga
     6421 tgctgccagc gatgggcctg ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc
     6481 tgatcggtaa cgtcatcaag gtacagttcc tcaatattcg tgactggcca tttggtgcag
     6541 ccaccagcat tacgctgact atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt
     6601 ctcgtctgct gaataagaag gtgaacgaat tagatgatta atcctttata ttcaatttat
     6661 taggtttgtt tttgagtgat tttgtcgttt aaggtaacgg cgttatagat cctatcaaaa
     6721 tcaatcaatt gcattctggt tttggagaaa gatttttcga gagcttcggt ctttaactaa
     6781 aactgattgc gtataatcgc aataccccct ccccgactat ctgaatggtc ggggaggcac
     6841 ttcaagcaca aaatatcatc cctgctccgt tgattactct acaggtgaag tcggccactc
     6901 aatatcaggt gctgctgatg tatcaacacg attcaacaac acccgatact ttttccaggc
     6961 ttcccgcaat gaagtctctt cctcaattac gatctccaga tctgcagcat cctgaagtgg
     7021 ggcgcaccac gcaaacatca caaaatattg tcgttgtgat ggacctgtgc aatagtgaat
     7081 cacgctacgt ttctgtgtac aacgtactgt agttttcttt acgatttata gctgtactgg
     7141 tgagccatga gtaatctgaa tccatgcatg gcgtgtggtg cctgttgtgc atttttctgc
     7201 gtctctttta ctggtccgaa gccgatgatg ctgaagcagt cctctcctct agcctcatcc
     7261 ctgtatagca cgcgtcaagt aagctgggat atcatttcgt agttttacaa tatcaatatc
     7321 cgaatcgata tgtttctggt aaaacttgtc cgcaagcaag tttgctatgg tatctgatgt
     7381 acgatttttt acatcccggc tcataccatc acaaagagca tccattttct tgccaaaaga
     7441 agcactcccc attattacat gattaataga actttgtgct gccttaacat caagggttag
     7501 ttgattctcc tggctcacaa gagaaaagac tctttgcctc tcttcagtgg aaatttttgc
     7561 atttttttga aaacagtaat tatcaatctc ctggttgatc acccttgagg gatatactat
     7621 gcgttcatct tttatccacc catcaatatc tttagcactc tgcctgttca ctgtatccag
     7681 tatatttctg ctaaatttgc gaacagaaaa gctaatagtc cgttctccaa ttgaacattt
     7741 tacgacctca tcattcttcc ctcggaaaga gttcctaaag gagccttctc tatggcaact
     7801 tataccaaaa gagctgaaag acgtacctga agtattcatc ggcattataa ttccttttta
     7861 taaaaaagcg tttacaagat tgcatgcttg tgtttattgt agccagaaaa aacatgcatg
     7921 caaataatat tctattaaaa gttgtattgt ttgagtccca tctcatgatc ggtatgttga
     7981 ctcttgttac caatatagtt cacaaaatgg gttgaaaagg ataataatat gccagtcata
     8041 ttaaattttt ctaatggaag tgtattgccg gaaaatgagc tggaggcttt acgacatatt
     8101 gcaagaagca atcagaatga cactattact ataggaggcc gtaatatgag gcttcattat
     8161 atccagttta tggatggttt tagtgtagag cctattcctg gtggactttg ggaccatctt
     8221 ggggcaagag agtctcatca tctcgcagac agcctggcaa gacaacttaa cggaggtaac
     8281 acttttctgc aggcatacag cttatatctg gagcagaggc aagctgcccc gcttgtgcag
     8341 gaaagcgtca taaaaacact actagatcga ataaattcga atgcatttcc cgttagttta
     8401 caagactttt cctgtactga ggagcatctt aattgtccga taacgctaca tattcctgag
     8461 acaggtgttt ttgtcagaaa tgctcgaaat tcagaaatat gtgcattata tgatcaggaa
     8521 gcattgactg aacttatcct gcgtaacgct ctccaccccc tcagccgtga cccctttgct
     8581 ccagaaatga ttataagcaa agacaagtgt cattttaata taacaaaaca atgtttttac
     8641 gcattaccca tataccccct tcaacaaaac agtatttaaa aataaaaaca caatataaga
     8701 agcatacaat aagttaatca ccatataaca aacatatagc gaggagatta tttatgccca
     8761 aaatatcatc agttgtatca tcatgttacc atctgttcag tgaacatcaa caactttcaa
     8821 atgaaacaac aatgacgaac t
//
LOCUS       DAZKGS010000172         8792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKGS010000172 DAZKGS010000000
VERSION     DAZKGS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..1316
                     /gene="moeA"
                     /locus_tag="ACHOQY_004095"
     CDS             81..1316
                     /gene="moeA"
                     /locus_tag="ACHOQY_004095"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9680082.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            1316..2065
                     /gene="moeB"
                     /locus_tag="ACHOQY_004096"
     CDS             1316..2065
                     /gene="moeB"
                     /locus_tag="ACHOQY_004096"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9680083.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2141..2803)
                     /gene="fsa"
                     /locus_tag="ACHOQY_004097"
     CDS             complement(2141..2803)
                     /gene="fsa"
                     /locus_tag="ACHOQY_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9680084.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGMAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            2934..3833
                     /locus_tag="ACHOQY_004098"
     CDS             2934..3833
                     /locus_tag="ACHOQY_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9680085.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3839..6271
                     /locus_tag="ACHOQY_004099"
     CDS             3839..6271
                     /locus_tag="ACHOQY_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP9680086.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6417..7232
                     /gene="ybiV"
                     /locus_tag="ACHOQY_004100"
     CDS             6417..7232
                     /gene="ybiV"
                     /locus_tag="ACHOQY_004100"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9680087.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7384..8649
                     /locus_tag="ACHOQY_004101"
     CDS             7384..8649
                     /locus_tag="ACHOQY_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP9680088.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         2084 a         2337 c         2351 g         2020 t
ORIGIN      
        1 acgcccgagg tggcatacag tctgtggcgt ttttctgaca taataggcaa attcgatttt
       61 gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc
      121 ttaatgagat gctttctcgc gtcaccccac tgaccgccca ggaaacgctg ccactggtac
      181 agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc gcttgatgtt ccggggtttg
      241 ataactccgc aatggacggc tacgcggtgc gtttagccga tattgcctcc aggcaaccgc
      301 tgcccgttgc cggtaaatcc tttgccggtc agccgtatca tggtgaatgg cctgcgggca
      361 cctgcattcg tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc
      421 aggagcagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc
      481 aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa
      541 ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg
      601 tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc
      661 agccgctggg cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag
      721 aacagctggg ttgcgaggta atcaacttag ggattatccg cgacgatccc catgccctgc
      781 gcgccgcatt tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt
      841 cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag atcgccttct
      901 ggaagctggc gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt
      961 tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc
     1021 ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac
     1081 gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc
     1141 aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat
     1201 ttagctcctt tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg
     1261 aagtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatgac
     1321 ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga
     1381 ttttgacggt caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact
     1441 cggctgcgca gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga
     1501 cttcgactcg gtttcgctct cgaatctgca acgccagacg ctgcacagtg atgctacggt
     1561 cgggcaaccg aaggtagaat ccgcccgtga cgccatgacg cggatcaacc cacatatcgc
     1621 gattacgcca gtcaatgcac tgcttgatga cgctgaactt gctgcgatga ttgcaaaaca
     1681 cgatctggta ctcgactgta cggataacgt tgcggtgcgt aatcaactga acgctggctg
     1741 ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac
     1801 ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga
     1861 gaacgcatta acctgcgtag aagcaggcgt gatggcaccg ttgatcggcg taattggttc
     1921 gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa
     1981 aatcgtcatg tacgatgcaa tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc
     2041 ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc cagataagac gcggcactcc
     2101 gccacgtcat cagcaattta tgaggatgca gaacgtccgg ttaaatcgac gttctgccaa
     2161 acgctcccag ccagtcctgt tcaaacttcg ccacagcggc ttccaccgcc ggatagctaa
     2221 tcatctgttg tgccacatcc agtggcagcg taattgattc acatcctgcc agtaagcagt
     2281 ccagcgcctg acgcggggtt ttgaaactcg ctgccagcac tttcgcctgc ggcgcatgca
     2341 ttttcaataa ctggtgtaag tcggtcacag tctgaatgcc gctaccgccc tgagcatcaa
     2401 tacgattaac gtaaggcgca acatattccg cacctgccag cgccgacagc agcccttgtg
     2461 ctgcgccata taccgcggtt cccagcgttg gaatcccttc cgcttttaac atcttaatag
     2521 ctgccatccc ctcggcggtc accggaactt tcaccacgat atccgcaata atagaacgca
     2581 atttacgtgc gtcattcacc atcccttcag cagtggtagc cattacctgg gcaaacagac
     2641 gcccctgacc gcccatcgct tcatgaagtt gcggaagcac aacatccagc ggtttgttac
     2701 ccgcggcgat aatgcttggg ttagtggtca cacccgccag cggaaaaata cgtgacagcg
     2761 ccttcaccgc aacaacgtct gaagtatcca gatacagttc cataaccatc ctcaaaatgt
     2821 actttttgtc tgaatgtaga ctatcacggg aaaactctct gaacagttga cgcacatcaa
     2881 tctaactttc attcgaaagc attttaatgt ttgaacgaaa cagagaggca actatgattt
     2941 tcaatattca gcgctactcg acccatgatg gccccggtat ccgcacggtc gtatttctta
     3001 aaggctgttc gctgggctgc cgctggtgtc agaacccgga aagccgcgcc cgcacgcagg
     3061 atctgctgta tgacgcacga ctgtgtctgg aaggctgcga gctgtgcgct aaggccgcgc
     3121 cggaagtgat tgagcgcgcg ctgaatggtt tgcttattca tcgggaaaag ttaaccccgg
     3181 agcatctgac ggcgttaacc gactgctgtc cgacacaggc attaaccgtg tgtggtgaag
     3241 tgaaaagcgt tgaggagatc atgacgaccg ttctgcgcga taaaccgttt tatgatcgca
     3301 gcggcggcgg tttaacgctt tcgggtggtg agccctttat gcagccggaa atggcgatgg
     3361 cgctactgca agccagccac gaggcaggca ttcatactgc ggtagaaacc tgtctgcatg
     3421 tgccgtggaa atatatcgcc ccttctctgc cctatatcga tctgtttctt gccgatttaa
     3481 aacacgttgc cgacgcgccg tttaaacagt ggaccgacgg taacgccgcc agagtgctgg
     3541 ataacctgaa aaaactcgcc gcagcgggca aaaaaatcat tatccgcgtg ccgctgattc
     3601 agggctttaa tgccgacgaa acctctgtaa aagccattac cgattttgcc gccgacgagc
     3661 tgcacgttgg cgaaattcat tttctgccct accacacgct gggcatcaac aaatatcact
     3721 tacttaatct gccctatgac gccccggaaa aaccgcttga tgcgccagaa ctgctcgact
     3781 ttgcccagca gtatgcctgc cagaaagggt taaccgcgac cttacgagga taacaaccat
     3841 gaccacactg aaactggaca cgctcagcga ccgcattaaa gcgcacaaaa atgcgctggt
     3901 gcatattgtg aaaccgccag tctgtaccga gcgcgcgcag cactataccg agatgtatca
     3961 acaacatctc gataagccga tcccggtacg tcgcgcgctg gcactggcgc atcacctggc
     4021 gaatcgcacc atctggatca aacacgatga gttgatcatt ggcaaccagg caagcgaagt
     4081 tcgcgccgcg ccgatcttcc cggaatatac tgtctcgtgg atcgaaaaag agattgatga
     4141 tctggcagat cgtcccggtg ctggctttgc ggtgagcgaa gagaacaaac gcgttctgca
     4201 tgaagtgtgc ccgtggtggc gcggtcagac cgtacaggat cgctgctacg gcatgtttac
     4261 cgatgagcaa aaaggtctgc tggcgaccgg aatcattaaa gcggaaggca atatgacctc
     4321 cggcgatgcg cacctggcgg tgaatttccc gctgctgctg gaaaaaggtc ttgatggtct
     4381 gcgtgagaaa gtagcggaac gtcgctcgcg catcaatctg acggtgctgg aagatttaca
     4441 tggcgaacaa ttcctgaaag cgattgatat cgtgctggtg gcagtcagtg aacacattga
     4501 acgtttcgct gccctggcgc gtgaaatggc tgctaccgaa actcgcgaaa gccgtcgcga
     4561 tgaactgctg acgatggcag aaaactgcga tcttatcgcc caccagccgc cgcagacttt
     4621 ctggcaggcg ctgcaactgt gttacttcat ccaattgatt ttgcagatcg aatctaacgg
     4681 tcactcggta tcgtttggtc gtatggacca gtatctctac ccgtactatc gccgcgacgt
     4741 tgaactcaac cagacgctgg atcgcgaaca cgccatcgag atgctgcata gctgctggct
     4801 gaaactgctg gaagtgaaca agatccgctc cggctcacac tcaaaagcct ctgcgggaag
     4861 tccgctgtat cagaacgtta ccattggcgg acaaaatctg gttgatggtc aaccaatgga
     4921 tgcagtgaat cccctctctt acgcgatcct cgaatcctgc ggtcgcctgc gttccactca
     4981 gcctaacctc agcgtgcgtt accatgcagg aatgagtaat gatttcctcg acgcctgcgt
     5041 acaggtgatc cgttgcggct tcgggatgcc ggcgttcaac aacgacgaaa tcgtgatccc
     5101 ggaatttatt aaactcggta ttgaaccgca ggacgcttac gactacgcag cgattggttg
     5161 tatcgaaacc gccgtcggtg gcaaatgggg ctatcgctgt accggcatga gctttatcaa
     5221 cttcgcccgc gtgatgctgg cggcgctgga aggcgggcgt gatgccacca gcggcaaagt
     5281 gttcctgcca caagaaaaag cgttgtcggc aggcaacttt aacaatttcg atgaagtaat
     5341 ggacgcgtgg gatacgcaaa tccgttatta cacgcgtaaa tcaatcgaaa tcgaatatgt
     5401 cgtcgacacc atgctggaag agaacgtgca cgatattctc tgctcggcgc tggtggatga
     5461 ctgtattgag cgagcgaaaa gtatcaagca aggcggcgcg aaatatgact gggtttctgg
     5521 cctgcaggtt ggcattgcca acctcggcaa cagcctggcg gcagtgaaga aactggtatt
     5581 tgaacagggc gcgattggtc agcaacagct ggctgccgca ctggcggatg acttcgatgg
     5641 cctgactcat gagcaactgc gtcagcggct gattaacggt gcgccgaagt acggcaacga
     5701 cgatgatact gtcgatacgc tgctggctcg cgcttatcag acctatatcg acgaactgaa
     5761 acagtaccat aatccgcgct acggtcgtgg tccggttggc ggcaactatt acgcgggtac
     5821 gtcatctatc tccgctaacg taccgtttgg cgcgcaaact atggcaacgc cggacgggcg
     5881 taaagcccac accccgctgg cagaaggcgc tagtccggcc tctggtactg accatctcgg
     5941 ccctactgcg gtcattggct cggtgggtaa actgcctacg gcagcgattc tcggcggcgt
     6001 gttgctcaac cagaaactga atccggcaac gctggagaac gaatctgaca agcagaagct
     6061 gatgatcctg ctgcgcacct tcttcgaggt gcataaaggc tggcatattc agtacaacat
     6121 cgtttcccgc gaaacgctgc tggaggcgaa aaaacatccg gatcagtacc gcgatctggt
     6181 agtgcgtgtc gcgggttatt ccgccttctt caccgcgctc tctccagacg ctcaggacga
     6241 tatcatcgct cgtactgaac atatgctgta atctcttcgg cccggctgcg gcgtcgccgg
     6301 gctaaatcat tcacatcatc aatttcatcc ttactttcat tcgaaatata atttgtgctc
     6361 tgcgtcacat tgttattaca ctgttacggt ttcattttcg agccaggagc acacctatga
     6421 gcgtaaaagt tatcgtcaca gacatggacg gtacttttct taacgacgcc aaaacgtaca
     6481 accaaccacg ttttatggcg caatatcagg aactgaaaaa gcgcggcatt gagttcgttg
     6541 tcgccagcgg taatcagtat taccagctta tttcgttctt tcctgagcta aaggatgaga
     6601 tctcttttgt cgcggaaaac ggcgcactgg tttacgaaca tggcaagcaa ctgttccacg
     6661 gcgaactgac ccgacatgaa tcgcggattg ttattggcga gttgctaaaa gataagcaac
     6721 tcaattttgt cgcctgcggt ctgcaaagtg catatgtcag cgaaaatgcc cccgaagcat
     6781 ttgtcgcact gatggcaaaa cactaccatc gcctgaaagc tgtaaaagat tatcaggaga
     6841 ttgacgacgt actgttcaag ttttcgctca acctgccaga tgaacaaatc ccgttagtga
     6901 tcgacaaact gcacatagcg ctcgatggca ttatgaaacc cgtcaccagt ggttttggct
     6961 ttatcgacct gattattccc ggtctacata aagcaaacgg tatttcgcgg ttactgaaac
     7021 gctgggatct gtcaccgcaa aatgtggtag cgattggcga cagcggtaac gatgcggaga
     7081 tgctgaaaat ggcgcgttat tcctttgcga tgggcaatgc tgcggaaaac attaaacaaa
     7141 tcgcccgtta cgctaccgat gataataatc atgaaggcgc gctgaatgtg attcaggcgg
     7201 tgctggataa cacatcccct tttaacagct gacctctcta ccggagccgt gcgctccggt
     7261 tttcccttct ttttacatcc tgtacatcac atcaaatcat taaacttgca ttaacttatt
     7321 tataacttat attgtcacat gcaaatcaga atggtttcga atgaaagtta atgagaggtg
     7381 gttatggctt ctacttttac cagcgacaca ttgcctgccg atcacaaagc agctatccgt
     7441 cagatgaagc acgcgctgcg ggcgcagctt ggcgacgtcc agcagatctt taatcagcta
     7501 agcgatgaca ttgccacgcg agtggctgaa atcaacgcac tcaaagcaca gggcgatgcc
     7561 gtctggccgg tgctgtctta tgccgatatc aaagcaggtc atgttactgc agagcagcgc
     7621 gaacagatta aacgtcgcgg ttgtgcggtg ataaaaggcc atttcccccg cgaacaagcg
     7681 ctaggctggg atcagtcgat gctggactat ctggaccgca accgctttga cgaggtctac
     7741 aaaggccccg gcgataattt cttcgggacg ctcagcgctt cacgtcccga gatttacccc
     7801 atctactggt cgcaggcgca aatgcaggcc cgccagagtg aagaaatggc gaatgcgcag
     7861 tcgtttctca atcgtctgtg gacatttgaa agtgatggaa agcaatggtt taacccggat
     7921 gtgagcgtca tctaccctga ccgtatccgc cgccgtccgc ccggaacgac ctccaaaggt
     7981 cttggagcgc ataccgactc cggggcgctg gaacgctggc tgcttccagc gtatcagcgc
     8041 gttttcgcca acgtctttaa tggcaatctg gcgcaatatg atccctggca tgcggcacat
     8101 cgtacggaag ttgaagagta cacggtggac aacaccacca aatgttccgt gtttcggaca
     8161 ttccagggct ggacagcgct ctctgatatg ctgcctggtc aggggctgct gcacgttgtg
     8221 cccattcctg aagccatggc gtacgtactg ttacgtccgc tgcttgatga tgtgccggag
     8281 gatgaactgt gcggcgtagc gcccggaaga gtgttgccgg tatcagagca atggcatcca
     8341 ctgttaattg aggcgttaac cagcattcca aaactcgagg ccggagactc cgtctggtgg
     8401 cactgcgacg tcatccattc cgttgccccc gttgaaaatc aacagggttg gggcaacgtg
     8461 atgtacattc ctgcggcacc gatgtgcgag aaaaatcttg cctacgcgca caaggtgaag
     8521 gccgcactgg aaaaaggcgc atcgccgggc gacttcccgc gcgaggacta tgaaacaaac
     8581 tgggaaggac gctttacgct tgccgacctc aacattcacg gtaagcgagc gttgggcatg
     8641 gatgtttgag agttaatgga agtgaattga tgtgggatta agaaaatcga ttcgacgtta
     8701 ctcagattgc tgacaagtgc gctttcttaa tgcctgatgc gctacgctta tctggcctac
     8761 aaagtcctgc agattcaata tattacaggt ac
//
LOCUS       DAZKGS010000173         8742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKGS010000173 DAZKGS010000000
VERSION     DAZKGS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..1138
                     /gene="allD"
                     /locus_tag="ACHOQY_004102"
     CDS             89..1138
                     /gene="allD"
                     /locus_tag="ACHOQY_004102"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9680089.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1160..2395
                     /gene="allC"
                     /locus_tag="ACHOQY_004103"
     CDS             1160..2395
                     /gene="allC"
                     /locus_tag="ACHOQY_004103"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9680090.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            2406..3191
                     /gene="allE"
                     /locus_tag="ACHOQY_004104"
     CDS             2406..3191
                     /gene="allE"
                     /locus_tag="ACHOQY_004104"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP9680091.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(3320..4465)
                     /gene="glxK"
                     /locus_tag="ACHOQY_004105"
     CDS             complement(3320..4465)
                     /gene="glxK"
                     /locus_tag="ACHOQY_004105"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP9680092.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4487..5787)
                     /locus_tag="ACHOQY_004106"
                     /pseudo
     CDS             complement(4487..5787)
                     /locus_tag="ACHOQY_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(5844..7205)
                     /gene="allB"
                     /locus_tag="ACHOQY_004107"
     CDS             complement(5844..7205)
                     /gene="allB"
                     /locus_tag="ACHOQY_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP9680093.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(7265..8719)
                     /locus_tag="ACHOQY_004108"
     CDS             complement(7265..8719)
                     /locus_tag="ACHOQY_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9680094.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         2337 a         2297 c         2114 g         1994 t
ORIGIN      
        1 ttttgcctgt ctggatcaca taatccagat attttccctg tatgttaatt gcagtcatgc
       61 ttcacaccgt cgttaaaaag gaagacagat gaaaatcagt cgggaaacac tccaccagct
      121 aattgagaat aaactctgcc aggctgggtt aaaacgtgag cacgctgcaa ccgtggctga
      181 agtattggtt tacgccgatg ccagagggat ccactctcat ggcgcggtgc gcgtggaata
      241 ctacgcggaa cgcatttcaa aaggcggcac caaccgcgaa ccggagtttc gtcttgagga
      301 aaccgggccg tgctcggcaa ttttacatgc cgacaatgcc gccggacagg tcgcggcgaa
      361 aatgggtatg gaacatgcca tcaaaaccgc ccagcaaaat ggcgttgcgg tggtcggtat
      421 cagccggatg ggtcacagcg gcgcaatctc ttattttgtg cagcaggcag cccgcgccgg
      481 attaattggt atttcgatgt gccagtccga tccaatggtg gtgccgtttg gcggcgcgga
      541 aatttactac ggtactaacc cactggcctt tgccgcgccg ggagaaggcg acgagatcct
      601 tacctttgat atggcgacta ccgtacaggc atggggaaaa gtgctcgacg cccgctcgcg
      661 taatatgtct atcccggata cctgggcggt cgataaaaac ggtgcaccaa caaccgatcc
      721 gttcgcggta catgctctgc tccccgccgc cgggccaaaa gggtatggcc tgatgatgat
      781 gattgacgtc ctctcaggcg tcttactcgg cttaccgttc gggcgacagg ttagttcgat
      841 gtatgacgat ttacacgccg ggcgtaattt ggggcaatta catatcgtta ttaacccgaa
      901 ctttttcttc tccagcaaat tatttcgtca acatcttagc cagaccatgc gcgaattaaa
      961 tgccattacc cccgcgcccg gttttaatca ggtttattat cccggacagg atcaggatat
     1021 taaacaacgc aaagccgccg tcgaaggcat cgaaattgtt gatgatattt accagtattt
     1081 aatttccgac gcgctttata acacgtcata cgaaacgaaa aatccctttg cgcaataatt
     1141 attgagacag gactttctta tgattacaca ttttcgtcaa gccatagaag aaacgctgcc
     1201 ctggctttcc tcttttggcg ctgacccaac gggtggtatg acccgtttac tttattcgcc
     1261 ggaatggctg gaaactcagc agcaatttaa aaaaagaatg gcagcaagcg ggctggaaac
     1321 acgtttcgat gaagtgggga atttatatgg tcgcctgagt ggcaccgaat atccacaaga
     1381 agtggttctg agcggttcgc atatcgatac cgtggttaac ggcggtaacc tcgacgggca
     1441 attcggcgcg ctggcggcgt ggctggcaat tgactggctg aaaacgcaat acggcgcgcc
     1501 gctacgtacg gtcgaagtgg tggcgatggc agaagaagaa ggcagccgct tcccgtatgt
     1561 cttctggggc agtaaaaata tctttgggct ggcgaatcct gacgacgtgc ggaatatctg
     1621 tgatgccaaa ggaaatagtt ttgtcgatgc gatgaaggct tgcggattta ctcttccgaa
     1681 cgccccacta actccgcgtc aggatattaa agcctttgtt gaactgcata ttgaacaggg
     1741 ctgtgtgctg gaaagtaatg ggcaatcaat tggcgtggtg aatgcaattg tcgggcagcg
     1801 tcgttatacg gtaacgctga acggcgaatc aaaccatgca ggcaccacgc cgatgggtta
     1861 tcgtcgtgat acagtttacg ctttcagtcg catttgccat cagtcggtcg aaaaagcgaa
     1921 aaggatgggc gatccgctgg ttctgacctt tggcaaagta gagccgcgcc cgaatacggt
     1981 aaatgtggtg ccgggtaaaa ccacgttcac cattgattgt cgtcataccg acgctgccgt
     2041 gctgcgcgat ttcacccaac agttagaaaa cgacatgcgg gcgatttgcg atgaaatgga
     2101 cattggtatt gatatcgatt tatggatgga cgaagaaccc gtgccgatga ataaggagct
     2161 ggtcgccacc ctgacagaat tgtgtgaaag agaaaaactg aattaccggg tgatgcacag
     2221 tggtgccggg cacgacgcgc aaattttcgc gcctcgcgta ccaacctgca tgattttcat
     2281 tcccagcatc aacgggatca gccataaccc ggcggaacgc accaatatta ccgaccttgc
     2341 cgaaggggtc aaaacgttgg cactcatgct ttatcaactt gcctggcaga aataaggagt
     2401 cataaatggg atatttaaat aacgtcaccg gttaccgcga tgatttactg gctaaccgtg
     2461 cgattgttaa acacggtaat ttcgcactgt taaccccgga tggtctggta aaaaatatta
     2521 ttccgggctt tgaaaattgt gacgcgacaa tcctctccac gccaaagctg ggtgcctctt
     2581 ttgttgatta tctggtcaca ctgcatcaaa acggtggcaa ccaacagggc ttcggtggcg
     2641 aaggcattga aacgttcctg tatgtgatct ctggaaatat cactgccaaa gccgaaggca
     2701 aaacatttgc cttaagcgaa ggtggctatc tttattgccc accaggctcc ttaatgacgt
     2761 ttgttaacgc ccaggccgaa gacagccaaa tctttttata taagcgccgc tatgttccgg
     2821 tagaaggcca tgcaccgtgg ctggtttctg gtaatgccag cgaactggaa cgcattcact
     2881 atgaaggtat ggatgatgtg atcctgctgg atttcctgcc caaagagtta ggttttgata
     2941 tgaacatgca tatcctctct tttgcgccag gtgccagcca cggttatatc gaaacacacg
     3001 ttcaggaaca tggtgcctat attctttccg gtcagggggt ttataacctc gacaataact
     3061 ggatcccggt gaaaaaaggc gattacatct ttatgggcgc ttattcttta caggctggtt
     3121 atggcgtagg gcgtggtgaa gcgttcagct atatttactc gaaagattgc aaccgcgacg
     3181 tagagattta agtcatacca ctcgcctgat tatttttagc tatgttggat tttgccacgg
     3241 tttataccgt ggcttttttt gtagtaaatt caggtaaaaa aagatgcgaa ataaatcgca
     3301 tccgctttaa aggtaagggt cagtttttaa ttccctgacc tattttaatg gcgcaggcaa
     3361 tatttcgcgc gctgttgaag agattggttt caccgctggc gagcacttcg gctaaaggtg
     3421 ccagacgagg caaaatgctg aataccgcgt caatgccgta ctggtgcacc acttccacgc
     3481 catcacccaa tacgccagca atcccaatca ccgggacatt aaactgcttc gccaccgacg
     3541 caacgcccag cggcgcttta ccgcctgccg tttgcgagtc aatgcgccct tccccggtga
     3601 tcaccagtgc tgcgccctgc accgcctgct ccaggttgac cgcccgcagt acaatctcaa
     3661 tacccggttt aatatccgca ttgaggaata ccgccgccgc aatacccata ccgcccgcag
     3721 cgccgccgcc cgccatctgg cagacattaa tttcagtttg ctgttgtaaa acacgggcgt
     3781 aattttgcag cccctgttca agttcttcga ccatctccgg cgttgcccct ttttgcgggc
     3841 caaacaccgc cgccgcgccg cgtgccccta ccagcgggtt atcaacgtca catgctactt
     3901 caatatggca attcgccaga cgcggatcgc attcatccat ctcaatgctt gccacgcgcg
     3961 ctaaattacc gccgttagcc gccagcacct ggccgtcggc atcaaggaaa cgcacaccaa
     4021 gcgcctgagc catgcccata ccgccgtcga ccgtcgcact gccgccaatg ccgagaataa
     4081 tatggcgaat gccgttatcc agcgcatgac gaattaactc ccccgtacca aaactggagg
     4141 ccagcaacgg attacgcttt tcaggcgcga ccagcatcag gccacttgct gccgccatct
     4201 caatcaccgc cgttttcccg tcgccggtaa ggccataaaa agcattcact gtttcgccca
     4261 tcggcccgca gacttcaagc gtcacgatgt tgccgcccgt cgcggcgacc atcgcatcca
     4321 ccgtcccttc gccgccatcc gctatcggca aacagatata gttcgcatcg ggaaagaggg
     4381 tcgaaaaccc ggctttaatt gcctgacaac atttttctgc acttaagctc tctttaaaag
     4441 agtctggcgc aatgacaatc ttcattatca atcctttttc aacaaattat tcgatgcgtt
     4501 cccacggaat aagattatcc ataagaacag ccagtaaaat tccgaccagt aagccgttac
     4561 tgagcagagg acgcagcgtt aatggaaggt cttgcagata cacaggcggt aatgccatta
     4621 aaaatatgcc gacaaataac ggcaatgcga gtcgataaat attgcgagcg gtaaaggtta
     4681 tttgctggct aaacaccagc gcggaaaaga gtaaaggcag ataagaaacc agcatgaccg
     4741 cgctactcac gggtaaaggg atactgcaaa acagtcgtgt gagtgccgga accagcgcca
     4801 ccagcaggca aataacgctg ccataaataa acgaacgccg cgtgtaatcg cctgtctggg
     4861 ttaataaacc aatggatgaa acaaacggtg aaaatggaat taccgccagc ggtacggtta
     4921 tcagcgtcat aaatccggtc gccacaaagc tgcgacgata acgcgtattc ccgcgccctg
     4981 ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga tattcaccag
     5041 acctgtaatc accgccgtca gaataattcc cggagacaaa gcacctccac tgccgagcgg
     5101 gaaccactgc cagtgcaact caccggagag cgagtgcgaa gaaggaaagc aaaagtacca
     5161 caacaaccag ccggttatgg tgccgaccag caggccataa cgggcaaaac gttgcggcag
     5221 gaagataatc atccccagta ccaggcacat caccgccacc gagagcgcga acggcggtaa
     5281 ctgaatttta aaatttgggt cggctatgcc aaacggcagc ccgagcatac ctttgaaaaa
     5341 gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg acggcgtaaa
     5401 taaccgtgcc aggcgatggc ctaatccgct aaaaccaatc aacatcgtca gcacgccgga
     5461 gagcgcaatc cccaccgcca ggctggtagc gatatcgttg atcggtgtcc cgcgcgatgc
     5521 ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacccggct cttccataat
     5581 agcgcgacga tgaccgcaga acgcctgagc gaagcaggcc agtgcggtag caagaaaagc
     5641 atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa gtagcgtagg
     5701 aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg ataacaggct
     5761 ttcgcggctc actgcaaaat tgaacataaa cacaccttaa ccggagaatg cccgccgcaa
     5821 ggacgggcat tgcaggggcc agattactgc tgatgtttaa ggataaattg acctttcggc
     5881 gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat ggttttcgtg
     5941 atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata ttccagatcg
     6001 tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc atcttttcct
     6061 ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt cgccattaat
     6121 ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa catcacgtcc
     6181 atacagtttt gcagaccggc aataccgccc catgcttcca tgatgttgcc ggctttcatt
     6241 tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt aaacagtttt
     6301 tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt cgccagagtg
     6361 ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga ttcacaagta
     6421 acgtcctgac cttcctgacg tgcacgagtc acttcttcaa cgccttccgg gctgctgata
     6481 tggcaaacgt gcagacggca accggcaact ttcgccagat acagcacgcg gcgaatcgct
     6541 tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac gcgaccttca
     6601 cgtttcgctt cttcgcccag tgcgtcacag atcagcgcgt tttcgcagtg caccagcacc
     6661 ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc gtttacatca
     6721 cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt gaagccgaca
     6781 acgcccactt catccagctc atgcagacgg tcgatgttgt aagacaccag gccaccgagt
     6841 tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc aattgaagcg
     6901 cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt gataccacct
     6961 tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc cggttcagaa
     7021 atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc atccataact
     7081 tcttttgcat cgcccagatc ctgaccgata gcagcaattt tcccgccttt aacggcgata
     7141 tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat taaatcaaaa
     7201 gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc aatatcggga
     7261 ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt ttctttaata
     7321 aggcgtaggc cgcaaaggcg acgatgacgc cgacaaacca tgaaacacgc gataacggtt
     7381 ccataaagtg aataaactta ccgccaagag ataaaataac ggccaccaga gttactgaaa
     7441 acgcagtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg gtatacagtt
     7501 catcaagatt aatttgtccg cgcatcacca caaaataatg cgccatcatg acaccaatta
     7561 ccggaccaag cattccgccg ataatatcga ggaaaagata aatgctgtcc tgattttcca
     7621 ttaatttcca cgggcagatc agcaagctga taatactggc aatcagtacg ccgtttttat
     7681 aggtcagttt tgtcggtgca atggcggcaa tctgataacc ggctggaata atattaccgg
     7741 tcgcgttagt ggagatagtt gtcatcagaa taaccagtac cgcaaagaac gaggcgaaca
     7801 ggctgtccca acgctgaaca atatccagca cgttccaggt atcagcgccg tagtgaatac
     7861 tggctccggc aataatacat accccggcga ccgcaaacag aatataggcc acaactaaac
     7921 ccagcgtttg ccccagcgcc tgctcacgaa acgagtgggc gttttgcgta aagtcggatg
     7981 cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt aatcaccacc aggaacagga
     8041 agccaccgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt ggaccgatcc
     8101 cgaccagtga aatcgcccaa atcgccatac cgccgaaaac gatatagatg cacgggttaa
     8161 gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca acgttgacca
     8221 gccagaagat taagaaagta attaagcccg gtagagaaag gcctaacaga gtgaaatcac
     8281 caccgagagt taaaaatccc ggccagattt tgccaatcag aatcaagcag gccagtgacc
     8341 ccgcgtaaca ttgcaaacca aaccacatga tggcggcaat tccgcccctt aataatccgg
     8401 gaaacagtgc accacgtaca ccgtaagaag cacgcaggat catggcaaaa ggcacaccgt
     8461 atttactgcc cgcagcaccg tttaatacca ttaccgcggc aatgaaaaag gcgctgagga
     8521 taattgccag cataatacta aaggtagaca agccgagaat aaaaaagccg ccgaccatca
     8581 cataattggg aacgttatga accgaaccca tccataaggt aaaatagtta aatgttttcc
     8641 aggtccgctg gctttgcgtt ttcggcaata gatcttcgct atagccgcgt tgctggaata
     8701 gttttctctg atgttccata aatccctctt aatttcacgt at
//
LOCUS       DAZKGS010000174         8638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKGS010000174 DAZKGS010000000
VERSION     DAZKGS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            281..1597
                     /gene="fucP"
                     /locus_tag="ACHOQY_004109"
     CDS             281..1597
                     /gene="fucP"
                     /locus_tag="ACHOQY_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9680095.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQLAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1630..3405
                     /gene="fucI"
                     /locus_tag="ACHOQY_004110"
     CDS             1630..3405
                     /gene="fucI"
                     /locus_tag="ACHOQY_004110"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9680096.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3514..4932
                     /gene="fucK"
                     /locus_tag="ACHOQY_004111"
     CDS             3514..4932
                     /gene="fucK"
                     /locus_tag="ACHOQY_004111"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9680097.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4934..5356
                     /gene="fucU"
                     /locus_tag="ACHOQY_004112"
     CDS             4934..5356
                     /gene="fucU"
                     /locus_tag="ACHOQY_004112"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9680098.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5414..6145
                     /gene="fucR"
                     /locus_tag="ACHOQY_004113"
     CDS             5414..6145
                     /gene="fucR"
                     /locus_tag="ACHOQY_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9680099.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6189..7289)
                     /gene="rlmM"
                     /locus_tag="ACHOQY_004114"
     CDS             complement(6189..7289)
                     /gene="rlmM"
                     /locus_tag="ACHOQY_004114"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9680100.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7282..7677)
                     /locus_tag="ACHOQY_004115"
     CDS             complement(7282..7677)
                     /locus_tag="ACHOQY_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9680101.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7696..8613)
                     /gene="gcvA"
                     /locus_tag="ACHOQY_004116"
     CDS             complement(7696..8613)
                     /gene="gcvA"
                     /locus_tag="ACHOQY_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9680102.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         2160 a         2247 c         2130 g         2101 t
ORIGIN      
        1 aacaattaat acggtcatct ggtttgtgtt ttttatgatt tattttctga aacgggcatg
       61 aaatttcgat tattaaagtg atggtagtca cataaagtca ccttccagct aataagtgtg
      121 accgccgtca tattacagag cgttttttat ttgaaaatga atccatgagt tcatttcaga
      181 caggcaaata ttcactgata tgaagcccga actcgctggt tttgcacttt tgaaaacata
      241 accgattacg tgcttaagct tctgaaccta agaggatgct atgggaaaca catcaataca
      301 aacgcagagt taccgtgcgg tagataaaga tgcagggcaa agcagaagtt acattattcc
      361 attcgcgctg ctgtgctcac tgttttttct ttgggcggta gccaataacc ttaacgacat
      421 tttattacct caattccagc tggcttttac gctgacaaat ttccaggctg gcctgatcca
      481 atcggccttt tactttggtt atttcattat cccaatccct gctgggatat tgatgaaaaa
      541 actcagttat aaagcaggga ttattaccgg attattttta tatgccttgg gcgctgcatt
      601 attctggccc gctgcagaaa taatgaacta caccttattt ttagttggcc tatttattat
      661 tgcagccgga ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg tattagggcc
      721 ggaaagcagt ggtcacttcc gcttaaatct tgcgcaaaca tttaactcgt ttggcgcaat
      781 tatcgcggtt gtctttgggc aaagtcttat tttgtctaac gtgccacatc aatcgcaaga
      841 cgttctcgat aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc tggtattatc
      901 ggtacagaca ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat
      961 gctgacgaaa ttcccggcat tgcagagtga taatcacagt gacgccaaac aaggatcgtt
     1021 ctccgcatcg ctttctcgcc tggcgcgtat tcgccactgg cgctgggcag tattagcgca
     1081 attctgctac gttggcgcac aaacggcctg ctggagctat ttgattcgct acgctgtaga
     1141 agaaattcca ggtatgactg caggctttgc cgctaactat ttaaccggaa ccatggtgtg
     1201 cttctttatt ggtcgtttca ccggtacctg gctcatcagt cgcttcgcac cacacaaagt
     1261 cctggccgcc tacgcattaa tcgctatggc actgtgcctg atctcagcct tcgctggcgg
     1321 tcatgtgggc ttaatagccc tgactttatg cagcgccttt atgtcgattc agtacccaac
     1381 aatcttctcg ctgggcatta agaatctcgg ccaggacacc aaatacggtt cgtccttcat
     1441 cgttatgacc atcattggcg gcggtattgt cactccggtc atgggttttg tcagtgacgc
     1501 ggcgggcaac atccccactg ctgaactgat ccccgcactc tgcttcgcgg tcatctttat
     1561 ctttgcccgt ttccgctctc aaacggcaac taactgaaca tattttccga ataaagtgag
     1621 gaatctgtaa tgaaaaaaat cagcttaccg aaaattggta tccgcccggt tattgacggt
     1681 cgtcgcatgg gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg
     1741 gccgcactgc tgaccgagaa actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt
     1801 tccgatacct gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa attcagcagt
     1861 cagaatgtag gcctcaccat tacggtaacg ccttgctggt gctatggcag tgaaaccatc
     1921 gacatggatc caacccgccc gaaagccatt tggggcttta acggcactga acgtcccggc
     1981 gctgtttacc tggcagcggc tctggcagct cacagccaga aaggcatccc ggcattctcc
     2041 atttacggtc atgacgttca ggatgccgat gacacatcga ttcctgccga tgttgaagaa
     2101 aaactgctgc gctttgcccg cgccggtttg gccgtcgcca gcatgaaagg taaaagctat
     2161 ctgtcgctgg gcggcgtttc gatgggtatc gccggttcca ttgttgatca caacttcttt
     2221 gaatcctggc tgggaatgaa agtccaggcg gtggatatga ccgaactgcg tcgccgtatc
     2281 gatcagaaga tttacgacga agccgaactg gaaatggcac tggcctgggc tgataaaaac
     2341 ttccgctatg gcgaagatga aaataacaaa cagtatcaac gcaatgccga gcaacgccgc
     2401 gcagttctgc gcgaaagttt actgatggcg atgtgtatcc gcgacatgat gcaaggcaac
     2461 agcaaactgg ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg
     2521 ggcttccagg ggcaacgtca ctggaccgat caatatccca atggtgacac cgccgaagcg
     2581 atccttaaca gttcatttga ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa
     2641 aacgacagtc ttaacggcgt ggcaatgcta atgggtcacc agctcaccgg caccgctcag
     2701 gtatttgccg atgtgcgtac ctactggtca ccagaagcaa ttgagcgtgt aacggggcat
     2761 aaactcgatg gactggcaga acacggcatc atccatttga tcaactccgg ttctgctgcg
     2821 ctggacggtt cctgtaaaca acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg
     2881 gaaatctctc agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc
     2941 cacgaatact tccgtggcgg cggttactct tcccgcttcc ttaccgaagg cggcgtcccg
     3001 ttcaccatga cccgtgtcaa catcatcaaa ggcctgggac cggtactgca aatcgcggaa
     3061 ggctggagcg tggaattgcc gaaggatgtg catgacatcc tcaacaaacg caccaactca
     3121 acctggccaa ccacctggtt tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg
     3181 tactcggtaa tggcgaactg gggcgctaac catggggttc tgaccatcgg ccacgttggc
     3241 gcagacttta tcactctcgc ctccatgctg cgtatcccgg tatgtatgca caacgttgaa
     3301 gagaccaaag tgtatcgtcc ttctgcctgg gctgcgcacg gcatggatat tgaaggccag
     3361 gattaccgcg cttgccagaa ctacggcccg ttgtacaagc gttaatacat tactccctga
     3421 tgtgatgccc ggtcgctccg gctaccgggc ctgaacaagc aagagtggtt agccggataa
     3481 gcaatgttat ccggctatat tgcaggagcg attatgaaac aagaagttat cctggtactc
     3541 gactgtggcg cgaccaatgt cagggccatc gcggttaatc ggcagggaaa aattgttgcc
     3601 cgcgcctcaa cgcctaatgc cagcgatatc gcgatggaaa acaacacctg gcaccagtgg
     3661 tctttagacg ccattttgca acgctttgct gattgctgtc ggcaaatcaa tagtgaactg
     3721 actgaatgcc acatccgcgg tatcgccgtc accacctttg gtgtggatgg cgctctggta
     3781 gataagcaag gcaatctgct ctatccgatc attagctgga aatgtccgcg aacagcagcg
     3841 gttatggaca atattgaaca gttgatctcc gcacagcggt tgcaggctat ttctggcgtc
     3901 ggagccttta gtttcaatac gttatataag ttggtgtggt tgaaagaaaa tcatccacaa
     3961 ctgctggaac gcgcgcacgc ctggctcttt atttcgtcgc tgattaacca ccgtttaacc
     4021 ggcgaattca ctactgatat cacgatggcc ggaaccagcc agatgctgga tatccagcaa
     4081 cgcgatttca gtccgcaaat tttacaagcc accggtattc cacgccgact cttccctcgt
     4141 ctggtggaag cgggtgaaca gattggtacg ctacagaaca gcgccgcagc aatgctcggc
     4201 ttacccgttg gcataccggt gatttccgca ggtcacgata cccagttcgc cctttttggc
     4261 gctggtgccg accaaaatga acccgtgctc tcttccggta catgggaaat tttaatggtc
     4321 cgtagcgccc aggttgatac ttcgctgtta agtcagtacg ccggttccac ctgcgaactg
     4381 gatagccagg cagggttgta taacccaggt atgcaatggc tggcatccgg cgtgctggaa
     4441 tgggtgagaa aactgttctg gacggctgaa acaccctggc aaatgttgat tgaagaagct
     4501 cgtctgatcg cgcctggcgc ggatggcgta aaaatgcagt gtgatttatt gtcgtgtcag
     4561 aacgctggct ggcaaggagt gacgcttaat accacgcggg ggcatttcta tcgcgcggcg
     4621 ctggaagggt taactgcgca attacagcgc aatctacaga tgctggaaaa aatcgggcac
     4681 tttaaggcct ctgaattatt gttagtcggt ggaggaagtc gcaacacatt gtggaatcag
     4741 attaaagcca atatgcttga tattccggta aaagttctcg acgatgccga aacgaccgtc
     4801 gcaggagctg cgctgttcgg ttggtatggc gtaggggaat tcaacagccc ggaagaagcc
     4861 cgtgcacaga ttcattatca gtaccgttat ttctacccgc aaactgaacc tgaatttata
     4921 gaggaagtgt gaaatgctga aaacaatttc gccgttaatt tctcccgaac tattgaaagt
     4981 gctggcagag atgggacatg gagatgaaat tattttttcc gatgctcact ttcccgccca
     5041 ttcgatggga ccgcaggtga tccgcgctga tggcctgttg gtgagcgact tgctccaggc
     5101 gattatcccg ttatttgagc tggacagtta tgccccgccg ctggtgatga tggcagcggt
     5161 agaaggtgac actctcgatc ctgaagtaga acgacgttac cgtaatgcgc tttcactaca
     5221 agccccgtgt cctgacatca tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa
     5281 agcctttgcg atcgttatca caggcgaacg agcgaagtac gggaatattc ttttaaaaaa
     5341 aggggtaaca ccgtaatctc ataccggtac gcccgcatga cgcgggcggt tatcgaatga
     5401 tggggtgaaa aatatgaaag cggcacgcca gcaagcgata gtcgacctgc tgctgaacca
     5461 taccagcctg accacggaag ctctctctga acagctaaag gtcagtaaag aaaccattcg
     5521 tcgcgatctc aatgaattac agacgcaggg taaaattctg cgcaatcatg gacgcgctaa
     5581 atatatccac cgtcaaaatc aagacagtgg cgatcccttt cacatccggc tgaaaagcca
     5641 ttatgcgcat aaagcagata tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt
     5701 gatagcctta gacgccagtt caacttgctg gtatctggca cgccagttgc ctgacatcaa
     5761 cattcaggtc ttcaccaata gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat
     5821 tcaactgatc agttccggcg gcacacttga gcgcaaatat ggctgttacg tcaatccctc
     5881 gctgatttcc caacttaaat cgctggaaat cgatctgttt attttttctt gtgaagggat
     5941 cgatagcagc ggcgcactgt gggactccaa tgccatcaac gctgattaca aatcgatgct
     6001 attaaaacgt gccgcgcaat cgttgttatt gattgataaa agtaaattta atcgttcagg
     6061 ggaagcccgc atcgggcatc tggatgaggt aacgcacatt atttctgatg agcgccaggt
     6121 tgcaacttct ttggtaacag cctgacggaa agggtagcag gccggagacg acgccccggc
     6181 cttgcctgtt atcgctcgtc gcgacgacca cccaccgcag cccagatgcg gcggacgtgc
     6241 accgtcactt cttcgcgatc gtgatacaac tgccgcgcct gaatctgagc atttatgcca
     6301 tgttcatcaa gctgtgcctg aatatacgcc agattgtgtg atacttcttc gtagcgtttt
     6361 ttcatcggca gtttgagatt gaaaatggtt tcacggcacc agccattaac cagccactgc
     6421 gccatcaatg ccgcaacttt agccggtttt tcaaccatat cgcataccat ccaggagata
     6481 ttgctgcgtg tcggacggaa tttgaaaccg tcttcccgca gccacgtcac ctgcccggta
     6541 tccatcagac tttgcgccat cgggccgttg tcgacggaat aaacccacat gttgcgcttc
     6601 accagttggt aggtccagcc gcctgggcaa gcgcccaaat ccaccgccca catcccgttc
     6661 gccaggcgtt catcccactc atccgcagga ataaacacat gaaatgcctc ttccagtttg
     6721 agtgtggaac gactcggcgc atctgccgga aatttcaggc gcggaatgcc catatagaac
     6781 ggcgaattat tgttgctgta tgagtaaccg gtatagcagc aacctggtgc aatgaagaat
     6841 acatgcacaa ccggacgctt cggcgtttca tagttcgcca gcaccccggc atcgcgcaag
     6901 gcagcgcgta gcggcacggt gaatttacgg cagaatttca gcaactcttt gctttcgttg
     6961 gtatcggcaa cttcaacacg cagttcaccg cccttctcta ctacgccctg taacatgccg
     7021 acaatggggg taatacgatc ttctggcggc aaatgctgca ggagttcccc caccacaaac
     7081 cactggcggg caaaaattaa tgaactgaac ggcagctcac ggattaactt atcgccatca
     7141 tcaggttgat aacattcata aatgacataa cccgcattct ctttcacgcg ggcaaaaccg
     7201 aaaatttccc gctggccggc tttatcggta atttctgcgg cgcactcttt ttcaaagccc
     7261 ggacggcaca gcaatacaac cttattcatg acttacgccc ttgcgcttta aacggatagc
     7321 accaactaac attaacgccc agcccgcaag gaagctcacg ccgccaaccg gagtgacaaa
     7381 cgcccacaaa cgcagatggg acagtgccag gcaataaagg ctgccgctga acagcaccgt
     7441 gcctaacgcg aggaaaacgc tactccagta aaaccagata ctgatgcgac gctgcattgc
     7501 caccgccaga cctaagatcg ccagcgtatg aaacgcctgg tattcgaggc cggtctggat
     7561 ccagcccatc tcaacggccc ccatggtttt acttaacaca tgcgcgccaa aagcgcccag
     7621 agccacaaaa atgaagccgc taatggcggc gaaaatcagc ataaaacggc tggtcatggt
     7681 cgtaccctac gtaaattatt gttcataacg aaagcggaat ttttcttgtt cagcagcggc
     7741 tttcgccagg atccattggc gaaaggcggc tattttaccc agttctgcct ggctgtcatg
     7801 acaaaccaga taaaaagcgt ttttactgac cagaacatca ttaaacgggc aaacaagacg
     7861 tccggcttca atttcagatt gcgccatcac gttatttgcc agcgccactc cctgcccgtg
     7921 aatagccgct tgcagcacca tggcgctatg actaaaaatt ggcccttgct gaacgttgat
     7981 atgatttaac cccaactgtc gggtatatgt ctgccagtca cggcgtgacg catcatgtaa
     8041 taacgtatgt ttagccagat cttccggggt cttcaagggt ttttcgcctg tcagcagtag
     8101 cggcgaacac accggcaata aatattcggc gtacagtttt tccacccgta gccccggcca
     8161 gttgccccga ccataaaata tcgccacatc aacatcatcc gccagcttat cttcctgacg
     8221 atcaaccgcc tggattcgaa cgtcaattcc cggataagct gaattaaagc tggaaagtcg
     8281 cggaaccaac caatgaatgg cgaaactggg gagtaaactg accgtcaacg cccccttggc
     8341 gctacgggcc tggagtttac gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc
     8401 gaggaaatag ctttgccctt cctcggtcag caggagtgaa cgattacggc ggcggaacag
     8461 ttttagcccc aaaaaatcct caagagactt gatttgatga cttactgcgg cctgggtcac
     8521 aaaaagctct tctgctgcgc gagtgaaact taaatggcgt gctgcggcat caaaaactcg
     8581 taaggcattt agcggtggta atcgtttaga catggctttt aaactttgat gttaaatg
//
LOCUS       DAZKGS010000175         8637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKGS010000175 DAZKGS010000000
VERSION     DAZKGS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(296..559)
                     /gene="rpsT"
                     /locus_tag="ACHOQY_004117"
     CDS             complement(296..559)
                     /gene="rpsT"
                     /locus_tag="ACHOQY_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9680103.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            662..880
                     /locus_tag="ACHOQY_004118"
     CDS             662..880
                     /locus_tag="ACHOQY_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9680104.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            888..1829
                     /gene="ribF"
                     /locus_tag="ACHOQY_004119"
     CDS             888..1829
                     /gene="ribF"
                     /locus_tag="ACHOQY_004119"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9680105.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1872..4688
                     /gene="ileS"
                     /locus_tag="ACHOQY_004120"
     CDS             1872..4688
                     /gene="ileS"
                     /locus_tag="ACHOQY_004120"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9680106.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4688..5182
                     /gene="lspA"
                     /locus_tag="ACHOQY_004121"
     CDS             4688..5182
                     /gene="lspA"
                     /locus_tag="ACHOQY_004121"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9680107.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDQVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5270..5719
                     /gene="fkpB"
                     /locus_tag="ACHOQY_004122"
     CDS             5270..5719
                     /gene="fkpB"
                     /locus_tag="ACHOQY_004122"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9680108.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5721..6671
                     /gene="ispH"
                     /locus_tag="ACHOQY_004123"
                     /gene_synonym="lytB"
     CDS             5721..6671
                     /gene="ispH"
                     /locus_tag="ACHOQY_004123"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9680109.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6737..7651
                     /gene="rihC"
                     /locus_tag="ACHOQY_004124"
     CDS             6737..7651
                     /gene="rihC"
                     /locus_tag="ACHOQY_004124"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9680110.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7818..>8637
                     /gene="dapB"
                     /locus_tag="ACHOQY_004125"
     CDS             7818..>8637
                     /gene="dapB"
                     /locus_tag="ACHOQY_004125"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9680111.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1957 a         2153 c         2466 g         2061 t
ORIGIN      
        1 aattcaaaaa tagagtggtc ttaagtttaa tacctcaatg tgtatcacaa tatccacatt
       61 ctttgtaggg gagtctggag attgagtaga tattcttgtt cagaatgtat cagccgatgg
      121 ttctacgatt cttaagccac gaagagttca gaaagtgtaa cggcatgtct gcaaaagcca
      181 gcatcttttg actatctggc aaccggtagt gtgttctctc acgcatcata aaagcagcag
      241 gcataaaaaa acccgcttgc gcgggctttt tcacaaagct tcagcaaatt ggcgattaag
      301 ccagtttgtt gatctgtgca gtcaggttag ccttatgacg tgcagctttg tttttgtgga
      361 tcagaccttt agcagcctga cggtccacga tcggttgcat ttcgttaaat gctttctgtg
      421 cagcagcttt gtcgccagct tcgatagctg cgtatacttt cttgatgaaa gtacgcatca
      481 tagagcgacg gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg cgcttcttag
      541 ctgatttgat attagccaag gtccaactcc caaatgtgtt ctatatggac aattcaaagg
      601 ccgaggaata tgccctttta gccttctttt gtcaatggat ttgtgcaaat aagcgccgtt
      661 aatgtgccgg cactcgttac gtagtgatgg cgcaggattc taccagcttg cagggtgtga
      721 atacagcttt tccgcgataa aaattgcagc aggcggtcac tttcttcccg tgatttgcgc
      781 catggcaatg aaaagccact tctttctgat ttcggtactc aatcgccggt taaccttgac
      841 cgctgtacaa ggtatactcg gacgattttc actgttttga gccagacatg aagctgatac
      901 gcggcataca taatctcagc caggccccgc aagaagggtg tgtgctgact attggtaatt
      961 tcgacggcgt gcatcgcggt catcgcgcgc tgttacaggg cttgcaggaa gaagggcgca
     1021 agcgcaactt accggtgatg gtgatgcttt ttgaacctca accactggaa ctgtttgcta
     1081 ccgataaagc cccggcacgg ctgacccggc tgcgggaaaa actgcgttac cttgcagagt
     1141 gtggcgttga ttacgtgctg tgcgtgcgtt tcgacaggcg tttcgcggcg ttaaccgcgc
     1201 aaaatttcat cagcgatctt ctggtgaagc atttgcgcgt aaaatttctt gccgtaggtg
     1261 atgatttccg ctttggcgct ggtcgtgaag gcgatttctt gttattacag aaagctggca
     1321 tggaatacgg cttcgatatc accagtacgc aaactttttg cgaaggtggc gtgcgtatca
     1381 gcagcaccgc tgtgcgtcag gcgctggcgg atgacaatct ggctctggca gaaagtttac
     1441 tggggcaccc gtttgccatc tccgggcgtg tagtccacgg tgatgaatta gggcgcacta
     1501 taggtttccc gacggcgaat gtaccgctac gccgtcaggt ttccccggtg aaaggggttt
     1561 atgcggtaga agtgctgggc ctcggtgaaa agccgttacc cggcgtggca aacattggaa
     1621 cacgcccaac ggttgccggt attcgccagc agctggaagt gcatttgtta ggtgttgcaa
     1681 tggaccttta cggtcgccat atacaagtag tgctgcgtaa aaaaatacgc aatgagcagc
     1741 gatttgcgtc gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta accgcccgcg
     1801 aattttttgg gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg aaacacggaa
     1861 ccgagaatct gatgagtgac tataaatcaa ccctgaattt gccggaaaca gggttcccga
     1921 tgcgtggcga tctcgccaag cgcgaaccgg gaatgctggc gcgttggact gatgatgatc
     1981 tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg catgatggcc
     2041 ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt ctgaaagaca
     2101 ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct ggctgggact
     2161 gccacggtct gccgatcgag ctgaaagtag agcaagaata cggtaagccg ggtgagaaat
     2221 tcaccgccgc cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag gttgacggtc
     2281 aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg tacctgacca
     2341 tggacttcaa aactgaagcc aacatcatcc gcgcgctggg caaaatcatc ggcaacggtc
     2401 acctgcacaa aggcgcgaag ccagttcact ggtgcgttga ctgccgttct gcgctggcgg
     2461 aagcggaagt tgagtattac gacaaaactt ctccgtccat cgacgttgct tttcaggcgg
     2521 tcgatcagga tgcactgaaa gcaaaatttg ccgtaagcaa cgttaacggc ccaatctcgc
     2581 tggtgatctg gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc tctattgcac
     2641 ctgatttcga ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg gcgaaagatc
     2701 tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc ggcacggtaa
     2761 aaggtgcgga gcttgagttg ctgcgcttta cccatccgtt tatgggcttc gacgttccgg
     2821 caatcctcgg cgatcacgtt accctggatg ccggtaccgg tgccgttcac accgcgcctg
     2881 gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc gctaacccgg
     2941 ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc gtgaacgtct
     3001 tcaaagcgaa cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg ctgcacgttg
     3061 agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc atcttccgcg
     3121 cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag tcactgaaag
     3181 agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag tcgatggttg
     3241 ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggcgtaccg atgtcactgt
     3301 tcgtgcacaa agacacggaa gagctgcatc cgcgtactct cgaactaatg gaagaagtgg
     3361 caaaacgcgt tgaagttgac ggcatccagg cgtggtggga tcttgatgcg aaagagatcc
     3421 tcggcgatga agctgatcag tacgtgaaag tgccggacac attggatgta tggtttgact
     3481 ccggatctac ccactcttct gttgttgacg tgcgtccgga atttgccggt cacgcagcgg
     3541 acatgtatct ggaaggttct gaccagcacc gtggttggtt catgtcttcc ctaatgatct
     3601 ccaccgcgat gaaaggcaaa gcgccgtatc gtcaggtact gacccacggc tttaccgtgg
     3661 atggtcaggg tcgcaagatg tctaaatcca tcggcaatac cgtttcgccg caggatgtga
     3721 tgaacaaact gggcgcggat attctgcgtc tgtgggtggc atcaaccgac tacaccggtg
     3781 aaatggccgt ttctgacgag atcctgaaac gtgctgccga tagctatcgt cgtatccgta
     3841 acaccgcgcg cttcctgctg gcaaacctga acggttttga tccagcaaaa gatatggtga
     3901 aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa gcggcacagg
     3961 aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag cgtctgatgc
     4021 gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac cgtcagtaca
     4081 ccgccaaagc ggacagcgtg gcgcgtcgta gctgccagac tgcgctgtat cacatcgctg
     4141 aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa gtgtggggct
     4201 atctgccggg cgaacgtgaa aaatacgtct ttaccggtga gtggtacgaa ggcctgtttg
     4261 gtctggcaga cagtgaagcg atgaacgatg cgttctggga cgagctgttg aaagtgcgtg
     4321 gcgaagtgaa caaagtcatt gagcaagcgc gtgccgataa gaaagtgggt ggctcgctgg
     4381 aagcggcagt aaccttgtat gcagaaccgg aactggcggc gaaactgacc gcgctgggcg
     4441 atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat aatgacgcac
     4501 ctgctgatgc tcagcagagc gaagtactca aagggctgaa agtcgcgttg agtaaagccg
     4561 aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag gtggcggaac
     4621 acgcagaaat ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa aaacgtaagt
     4681 ttgcctgatg agtcaatcga tctgttcaac agggctacgc tggctgtggc tggtggtagt
     4741 cgtgctgatt atcgatctgg gcagcaaata cctgatcctc cagaactttg ctctggggga
     4801 tcaggtcccg ctgttcccgt cgcttaatct gcattatgcg cgtaactatg gcgcggcgtt
     4861 tagtttcctt gccgatagcg gcggctggca gcgttggttc tttgccggta ttgcgattgg
     4921 tattagcgtg atcctggcag tgatgatgta tcgctcgaag gccacgcaga agctaaacaa
     4981 tatcgcttat gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc gcctgtggca
     5041 cggcttcgtt gtcgatatga tcgacttcta cgtcggcgac tggcacttcg ccaccttcaa
     5101 ccttgccgat actgccattt gtgtcggtgc ggcactgatt gtgctggaag gttttttgcc
     5161 ttctaaagcg aaaaaacaat aataaaccct gccggataag gcctacagat tgctgcgaaa
     5221 tcgtaggccg catccggcaa aaatcctcaa aatataagag caaacctgca tgtctgaatc
     5281 tgtacagagc aatagcgccg tcctggtgca cttcacgctg aaactcgatg atggcaccac
     5341 cgctgagtct acccgcaaca acggtaaacc ggcgctgttc cgcctgggcg atgcttctct
     5401 ttctgaaggg ctggagcaac acctgttggg gctgaaagtg ggcgataaaa ccaccttctc
     5461 gctggagccc gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt acttctcccg
     5521 ccgtgaattt atggatgcag gcgagccaga aattggcgca attatgcttt ttaccgcaat
     5581 ggatggcagt gagatgcctg gcgtgatccg cgaaattaac ggcgactcca ttaccgttga
     5641 tttcaaccat ccgctggccg ggcagaccgt tcattttgat attgaagtgc tggaaatcga
     5701 tccggcactg gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg
     5761 gggtagaccg cgctatcagc attgttgaaa acgcgctggc catttacggc gcaccgatat
     5821 atgtccgtca cgaagtggta cataaccgct atgtggtcga tagcttgcgt gagcgtgggg
     5881 ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt ttctccgcac
     5941 acggtgtttc tcaggcggta cgtaacgaag caaaaagtcg cgatttgacg gtatttgacg
     6001 cgacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgccagt cgccgtggcg
     6061 aagaatctat tctcatcggt cacgccgggc acccggaagt ggaagggaca atgggccagt
     6121 acagtaaccc ggaaggggga atgtatctgg tcgaatcgcc ggacgatgtg tggaaactga
     6181 cggtcaaaaa cgaagagaag ctctccttta tgacccagac cacgctgtcg gtggatgaca
     6241 cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt ccgcgcaaag
     6301 atgacatctg ctacgccacg actaaccgtc aggaagcggt acgcgccctg gcagaacagg
     6361 cggaagttgt gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt ctggcggagc
     6421 tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagat atccaggaag
     6481 agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc
     6541 tggtgcagaa tgtggtggca cgtttgcagc agctgggcgg tggtgaagcc attccgctgg
     6601 aaggccgtga agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc gatattcgtg
     6661 aagtcgatta agtcattagc agcctaagtt atgcgaaaat gccggtcttg ttaccggcat
     6721 tttttatgga gaaaacatgc gtttacctat cttcctcgat actgaccccg gcattgacga
     6781 tgccgtcgcc attgccgccg cgatttttgc acccgaactc gacctgcaac tgatgaccac
     6841 cgtcgcgggt aatgtctcgg ttgagaaaac tacccgcaat gccctgcaac tgctgcattt
     6901 ctggaatgcg gagattccgc tcgcccaagg ggccgctgtg ccgctggtac gcgcaccgcg
     6961 tgatgcggca tctgtgcacg gcgaatcggg aatggctggc tacgactttg ttgagcacaa
     7021 ccgaaagccg ctcgggatac cggcgtttct ggcgattcgg gatgccctga tgcgtgcacc
     7081 agagcctgtt accctggtgg ccatcggccc gttaaccaat attgcgctgt tactttcaca
     7141 atgcccggaa tgcaagccgt atattcgccg tctggtgatc atgggtggtt ctgccggacg
     7201 cggcaactgt acgccaaacg ccgagtttaa tattgctgcc gatccagaag ctgctgcctg
     7261 tgtcttccgc agtggtattg aaatcgtcat gtgcggtttg gatgtcacca atcaggcaat
     7321 attaactcct gactatctct ctacactgcc gcagttaaac cgtaccggga aaatgcttca
     7381 cgccctgttt agccactacc gtagcggcag tatgcaaagc ggcttgcgaa tgcacgatct
     7441 ctgcgccatc gcctggctgg tgcgcccgga cctgttcact ctcaaaccct gttttgtggc
     7501 agtggaaact cagggcgaat ttacctcagg cacgacggtg gttgatatcg acggttgcct
     7561 gggcaagcca gccaatgtac aggtggcatt ggatctggat gtgaaaggct tccagcagtg
     7621 ggtggctgag gtgctggctc tggcgtcgta acctgtcaca tgttattggc atgcagtcat
     7681 tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt ctaattatca
     7741 gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc tatgcaaatt
     7801 aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg ggagccgggg
     7861 ggcgtatggg ccgccagttg attcaggcgg cgctggcatt agaaggcgtg cagctgggcg
     7921 cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag ctggctggtg
     7981 cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagat gattttgatg
     8041 tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt tgtcgccagc
     8101 atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa caggcgattc
     8161 gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt ggcgttaacg
     8221 tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc gatatcgaaa
     8281 ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca ctggcaatgg
     8341 gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc tacagtcgtg
     8401 aaggccacac cggtgaacgt gtgcctggca ccattggttt tgccaccgtg cgtgcaggtg
     8461 acatcgttgg cgaacatacc gcgatgtttg ccgatattgg cgagcgtctg gagatcaccc
     8521 ataaggcgtc cagccgcatg acatttgcta acggcgcggt aagatcggct ttgtggttga
     8581 gtggtaagga aagtggtctt tttgatatgc gagatgtgct tgatctcaat agtttgt
//
LOCUS       DAZKGS010000176         8628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKGS010000176 DAZKGS010000000
VERSION     DAZKGS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..972)
                     /gene="glxR"
                     /locus_tag="ACHOQY_004126"
     CDS             complement(94..972)
                     /gene="glxR"
                     /locus_tag="ACHOQY_004126"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9680112.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1072..1848)
                     /gene="hyi"
                     /locus_tag="ACHOQY_004127"
     CDS             complement(1072..1848)
                     /gene="hyi"
                     /locus_tag="ACHOQY_004127"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP9680113.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(1861..3642)
                     /gene="gcl"
                     /locus_tag="ACHOQY_004128"
     CDS             complement(1861..3642)
                     /gene="gcl"
                     /locus_tag="ACHOQY_004128"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9680114.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3732..4547)
                     /gene="allR"
                     /locus_tag="ACHOQY_004129"
     CDS             complement(3732..4547)
                     /gene="allR"
                     /locus_tag="ACHOQY_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9680115.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4625..5107)
                     /gene="allA"
                     /locus_tag="ACHOQY_004130"
     CDS             complement(4625..5107)
                     /gene="allA"
                     /locus_tag="ACHOQY_004130"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9680116.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5337..6263
                     /gene="allS"
                     /locus_tag="ACHOQY_004131"
     CDS             5337..6263
                     /gene="allS"
                     /locus_tag="ACHOQY_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9680117.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6332..7426
                     /gene="mnmH"
                     /locus_tag="ACHOQY_004132"
     CDS             6332..7426
                     /gene="mnmH"
                     /locus_tag="ACHOQY_004132"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9680118.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            7542..7907
                     /gene="ylbG"
                     /locus_tag="ACHOQY_004133"
                     /pseudo
     CDS             7542..7907
                     /gene="ylbG"
                     /locus_tag="ACHOQY_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(8428..>8628)
                     /locus_tag="ACHOQY_004134"
     CDS             complement(8428..>8628)
                     /locus_tag="ACHOQY_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680119.1"
                     /translation="TLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVG
                     EINVTHYFITNIGAGLPDACAE"
BASE COUNT         2200 a         2259 c         2031 g         2138 t
ORIGIN      
        1 atttttaata ctgaagcaac atctttggct gctttctttt ttaatattgc tggccgattc
       61 attttctgat atcggccatt tttattgcgg gtatcaggcc agtttatggt tagccattaa
      121 ttccagcgcc tgcactaacg cagagtgatc caactggctg ccaccgtttg ccgcgcaggt
      181 attaaataac tcctggcagg tcgcagtgtt tggcaggttc agcgctagcg ctttcgcact
      241 ttgcagtgcc aggttgagat ctttctggtg cagcgcaatt ttgaaacctg gattaaaagt
      301 gcgtttaatc atgcgctcac cgtgaacttc cagaatacgt gaggaagcaa agccgcccat
      361 cagcgcctgg cgcacacgta ccgggtccgc accggctttt gaagcaaata gcagggcttc
      421 agaaaccgct tcaatattga gcgccacaat aatctgattt gccactttgc aggtttgacc
      481 atcgccgtta ccgcccacga gggtgatatt tttaccgagc agttcaaaca gcggtttaac
      541 acgttcaaat accgcttcat caccgccaac cataatcgac aacgtccctt cacgcgcacc
      601 gatttcaccg ccggagactg gcgcatcgag ataatcgccg cccagttcat tcacctgacg
      661 agcgaaacgc ttagtttcaa tcggggaaat ggagctcata tcaacaatgg ttttgccctt
      721 cagcgaggct ttggtacaac cattttcacc gaacagaact tcttcaacct gtggtgtatc
      781 tggcaccata ataaaaatga tgtccgatgc ttccgttacc tggcgcgcag tttcaacact
      841 gacggcaccc agtgacagta attcatcagc aaccggacca atggttgtga catgcagttg
      901 atgaccggca cgcgccagat taatggccat cggtgtaccc ataatgccta agccaataaa
      961 tcccagtttc ataattaacc tcttttaaat ttcgcttttc ctgaattcag acaacacgat
     1021 cccctgccaa cttagcggcc taaaaaagca ttgcctgaac agcgttacgt tttaacggta
     1081 cggatccatc cagcgtaaac cggcttccgt ggtggtttgg ggtgtatatt cacacccaac
     1141 ccaaccgttg tagtcagaat tttcgattac cttaaagaga taatcataat taatttctcc
     1201 ggtccccggt tcgccgcgat gcggattatc agcaatttgc aggtgaccaa ttttatcggc
     1261 ccactgagtc atggtgttgg ttaattcacc ttccatccgc tgcatgtgat aaatgtcata
     1321 ctgaattttc aaattgcagc aaccaacatc atcaatcaat ttcagcgcct gccgagttcc
     1381 ggtgagatgg aaaccaggaa tatcaaaatg gttaataggt tcaatcagta ataaaatatc
     1441 ttctttcatc agcatattcg cggcataacg caggttttct acaagcgttg cgtgaatctg
     1501 ttcactgctg aaaccagccg gcgttttacc gaccagacag ttaatttttt tattacccag
     1561 cgcacgggca taacgaatcg ctgctgctac gccatcccga aactcttctt cacggccagg
     1621 aatacaggca ataccgcgct cccccgccgc ccagtcacct gccggtaaat tgtgcagcgt
     1681 atgttcgagt ttattactcg ccagcacctg ttttaattct tcaatgtcgt agtcataagg
     1741 aaacataaat tcaacgccgc gaaaaccaca ctgcgcagct ttctcaaaac gggcgagaaa
     1801 atcatattca ccaaataaca tcgataaatt agcagagaaa cgtaacatta tttatctccc
     1861 ttattcatag tgcatgaagc aggtttcagt cggtgcgtcc gctgcgttat cggcgatatc
     1921 ttcaaattcc atgacgttat ccagttcgct gcccatcgaa atattggtca cacgctcaag
     1981 aataacttcc acgactaccg gtacccgata ttgcgccatt aaggctttcg cctgttcaaa
     2041 ggctggcgca atatcttccg gtttgaagac ccgaatagct ttacaaccta aaccttccgc
     2101 tacttttacg tggtcgacgc cgtaaccgtt cacttcactg gagttaatat tttcgaacgc
     2161 gagctgtacg cagtaatcca tgtcaaaggc gcgttgtgac tgacgaatca gccccagata
     2221 agcgttattg accagtacat ggatgtacgg aatgttgaac tgtgcgccaa cggctaattc
     2281 ttcaatcagg aactggaagt cgaagtcacc ggaaatcgcc accacattcc gttctggatc
     2341 agcagcacac acgcccagcg ccgccggaat ggtccagccc agcgggcctg cctgaccaca
     2401 gttgatccag tgacggtctt taaagacatg cagcatttgt gccgcagcga tttgtgacag
     2461 gccaatggtg gtgacataac aaacatcgcg accaaaggct ttgttcatct cttcatacac
     2521 gcgctgcggt ttcaccggca cgttgtcgaa gtgggttttg cgcagcaaag tgcgtttgcg
     2581 ctgctggcag tcggcgaccc attctttacg acacggcaga cgacccgctt tttgcatctc
     2641 ctgcgccact tcaaccagca gtgtcagcgc cgctttagca tcagagacaa tgccgagatc
     2701 cggacacagc acgcgaccaa tttgcgtcgg ctcaatatcg atatgaacga ttttgcgccc
     2761 ttcggtgtat ttctctaccg aaccggtatg acggttagca aaacggttac cgataccaaa
     2821 caccatgtcg gacgccagca gcgttgcgtt accgtaacga tgcgcggttt gcagacccac
     2881 catcccggcc atcagttcat gatcgtccgg gatacagccc cagcccatca gcgttgggat
     2941 caccggaacg ctggtcagtt cagcaaactg ttgtaacagt gcagctgcgt cagcattaat
     3001 taccccgccc ccggcaacaa tcaccggacg ttcggcctgg attaacattt ctacagcttt
     3061 ttcgatctgc atacggctgg cagcaggttt gtagaccggc agcggttcgt acatatcagg
     3121 atcaaactcg atttccgcaa cctgaacgtc gaacggtaaa tccaccagta ccggacccgg
     3181 acgaccagaa cgcatcaggt gaaatgcctg ttgcaacaca cgaggcacca gcgccgcttc
     3241 acgtactgta accgccattt tacttaccgg cttggcgata gcttcgatat caacagcctg
     3301 gaaatcttct ttatgcagac gggcgcgcgg agcctggcca gtaatacaca gaataggaat
     3361 ggaatcagca gaagcagaat agagcgcggt gatcatgtcc gtgcccgcag gaccggaagt
     3421 ccccagacat acgccgatat tccctgccgt tgcgcgggta taaccttccg ccatgtgcga
     3481 agcaccttcc acatgacgcg ccagaatgtg acgaataccg ccgtgcttac gcatcgctga
     3541 gtagaacgga ttgattgcag ctcccggaac gccgaaggcg gtggtgatac cttctttctc
     3601 cagcacatac attgccgcgt caacggctct catttttgcc attttattcc aacctctatt
     3661 aattggaaaa attttccaac tttcaaattt tgtcaaaaaa acccgctccg cagagcggga
     3721 tgcgacagac actatggatg tgctttcagt cccaacgccg tgctgatatc gcgggcggtg
     3781 tctctgacca gctcaccctg actgacaaaa cgatcttctg tcagtcttga tgaaggcccg
     3841 gagatggaga tagcggcaac aacgctaccg acatcatcgt aaattgctga agctatgcaa
     3901 ttcagaccta caacatgctc ttctttatct acggtatagc ccagttcacg cgcttgttcc
     3961 aggtccttca gcaaggtggg catatccaca agcgtagttg gcgtaaactg ctgcaaaccg
     4021 gtttgcagaa tgatgctcat caactcctct tccgccagcg gataaagcaa cgccttgccc
     4081 gcaccggaag catgcagcgg cagacgactg cccagtggcg cacacatcct gaccatcgat
     4141 ttacactcta actgaccaat taataccgct tcattgccgt tacggatcgc gacattgacc
     4201 gtttcgccgg aaagtaacat caggcggcgc ataaacggcc cggcgacgga gaggacatcg
     4261 cggttatgga tgtacgccgc accgacgtta aagacaccta atcctatatg ccaccagcct
     4321 aactgactgt cctgatagac aaaatccgct gcctgtaaaa ccttcagcaa gcgaaaggtc
     4381 gtagagagcg gcaaatccag attgagagaa atatcgctaa ccgacgaact tcccccactt
     4441 ttttccagat attgcagaat cgcaattccc cgctctaacg cctgtgcgcc cttctgtgcc
     4501 acaggctccg cctgtcctgg cctgccgcgc cgtctaactt ccgtcatacc tacgttaact
     4561 ccgacctaca agtgagcata aacaatgaac aattagttta tcttatgcaa gtcggttgca
     4621 ggcgtcacgc aaaacagagt tcctgttcag gaatactttc aacatcacag ttgtcactgc
     4681 cgccgcgatc gatggtcaga aaatcggtga cgcgctgcca ggcgaaaagt gggtgatgcc
     4741 agacgttacg gtggtaattc actccctgtt cgccgttggt aataaacgcc cgcagggttg
     4801 acaggtctgg tttgtcgtca cctaacgcca cgaccaccac aaacacctca cctttcatcg
     4861 ggataaaggc ctgagtaccc agcggatgac gttcgagttc gtgaatggtc agcggcagat
     4921 tcgccggttg cgcgcggtta atgctgataa gcgtacggtc ttgctcaaga atctcaacca
     4981 gcgccaaatc gtggtaacgc tccaccaggc cattgttaat atggaaaaaa tcccgttgct
     5041 gcgtttcgat tacgtcgcca taagcactaa aggcttcctg acttaacggt aatacctgaa
     5101 gtttcatctt tttgtccttg taacataatt ccgttcaacc accgccgacc ggtcagcttg
     5161 gtttcaataa caggcaatct aaaaccttaa gagaacaaca tcaatacaaa agttggaata
     5221 tttttccaaa actagattta gtccacaaat tgaacagggt tatcatgtaa aaatactgat
     5281 aaatcagagg ataagaaaat cacgcaactt gcggatttaa aaggggatac ctgaagatgt
     5341 tcgatccaga aaccttgcgg actttcattg cggttgctga aacaggaagt ttttcaaaag
     5401 cggcagaacg attatgtaaa accacggcga cgatcagtta tcgcattaaa cttctggaag
     5461 agaataccgg agtagcgctg tttttccgca cgactcgcag cgtgacgttg acagcggctg
     5521 gcgagcatct actttcccag gccagagact ggctgagctg gctggaaagt atgccaagcg
     5581 agctgcaaca ggtgaatgat ggcgtggaac gccaggtgaa tattgtcatc aacaacctgc
     5641 tctacaaccc ccaggccgtc gcccagttgc tggcgtggct gaatgaacgt taccccttta
     5701 cccagtttca catctcccga caaatctata tgggcgtctg ggattcgcta ttatacgaag
     5761 gtttttcact ggctatcggc gtcacgggaa ccgaggcgct ggcaaatacc tttagtcttg
     5821 atcccttagg atcggtgcaa tggcgctttg tcatggcggc ggatcatccg ctggcgaacg
     5881 ttgaagagcc gctaacagaa gcgcagttgc ggcgctttcc ggcggtcaat attgaagaca
     5941 gcgcccgcac cttaaccaaa cgcgtcgcct ggcgattgcc tgggcaaaaa gagattattg
     6001 ttcctgatat ggaaacgaaa atcgccgccc atcttgcggg cgttggcatt ggttttttgc
     6061 caaaatcgct ttgccagtca atgatcgata atcaacaact agttagccgg gtaatcccaa
     6121 cgatgcgccc tccttcgcca ttgagtctgg catggcgcaa atttggcagc ggcaaagcgg
     6181 tagaagatat tgtgaccttg tttacccagc gcaggccgga aatcagcgga tttttagaaa
     6241 ttttcggcaa cccacgcagt taaaaatcaa gtttccttgc gcgttggcgc gtaaaatagc
     6301 gcgcaagctg atttgaggtt cacttttaac tatgcaagag agacacacgg aacaggacta
     6361 tcgtgccctg ctgattgctg atacgcccat tattgatgtt cgcgccccta tcgagtttga
     6421 gcaaggcgca atgcccgccg ctatcaatct gccgttaatg aataacgatg aacgcgccgc
     6481 cgttggcacc tgctataaac agcaaggctc agacgcagcg ctggcgctgg ggcataaact
     6541 ggtggcgggt gaaattcgtc agcagcgcat ggacgcctgg cgggcagcgt gcctgcaaaa
     6601 tccgcaaggt attctctgct gcgcccgtgg cggtcagcgc tcacatattg tgcaaagctg
     6661 gttgtatgca gcggggattg attatccgct ggtggaaggc ggttataagg cactgcgcca
     6721 gaccgcgatt caggcgacta ttgaactggc acaaaaaccg atagtgctga ttggcggttg
     6781 tactggcagc ggtaaaacgc tgttagtgca gcaacagccg aacggtgttg atctggaagg
     6841 gctggcgcgc catcgcggtt cggcgtttgg tcgcacgtta caaccacaac ttagccaggc
     6901 gagttttgaa aacctgctgg ctgccgaaat gctgaaaacc gacgcccgtc aggatttgcg
     6961 cctgtgggtg ctggaagacg aaagccggat gatcggttcg aatcacctgc cggaatgcct
     7021 gcgcgagcga atgactcagg cggcgattgc ggtggtagaa gatccgtttg agatccgtct
     7081 tgagcgcctg aacgaagagt atttcttgcg tatgcatcat gattttaccc acgcttatgg
     7141 cgacgaacag ggctggcagg aatattgcga atacctgcat cacggacttt cggcgattaa
     7201 gcgtcggctg gggttacagc gctataacga actcgctgcc cagctggata cggcgctgac
     7261 aacgcaactc accaccggca gcaccgacgg tcatctggcc tggctggtgc cgttacttaa
     7321 agaatattac gacccaatgt atcgctatca gcttgagaaa aaagcggaaa aagttgtctt
     7381 tcgcggtgag tgggcggaag tggcggaatg ggttaaggcg cggtaatgag ggacgtattt
     7441 gtattagtcg cgacttgatc tgacacatgg ccttgaaagg ttgagagtta ccggttttga
     7501 tatgagtgtc gaatccttat ccaaaacaag aggtaactct catgcttcat actgccaatc
     7561 ccgtcatcaa acacaaagcc ggtttgctca atctggctga agaactcagc aacgtatcaa
     7621 aagcctgtaa aatcatgggc gtttcgcgtg atacgtttta tcgttaccgc gaactggtcg
     7681 ctgaaggcgg tgtggatgcg cagattaatc gtagtcgccg cgctcctaac cttaagaacc
     7741 gtaccgatga ggcaactgaa caggctgttg ttgattacgc cgtcgctttc ccgacacatg
     7801 gtcagcaccg ggccagcaac gagctgcgta aacagggggt ttttatctcc gacagtggtg
     7861 tccgttctgt ctggttactc cataaccttg agaatctcaa aagacgatac tgaacaaatt
     7921 ttatcaggtg acgttccgta aaaagttgta tggagttctc gatgcagtcg gcgcgactat
     7981 cagtaccccc gctgaaccct ggactatcgg gccgccgtac tggggcatgt caccctggcg
     8041 tgccgctggt tttccgctaa tggtttattt cctctttctt tcccggcaag aatttatttt
     8101 aatccggaag gattatttat tttcagatag ggactcacat taaacacgta acattttaat
     8161 taatgcatgg ctgaatatgt attacacaat gtcagatatc gtaaaagacc agtgcaattc
     8221 tatgtaaact ctctgactgt tcattttatt tgttgtttca gggtcggaac tcaccctcaa
     8281 taaacaactg aatacgagta gatattcatc gtctgagcta tatggcttta cacaatagcc
     8341 gacatgtctt ttctcagtca ggaattttac ttttcctcac tacgaagttg acgacttcga
     8401 tatggaatag actcttaatt caagcaatta ctctgcacaa gcatcaggca atccagctcc
     8461 aatatttgtt ataaaataat gcgtaacatt aatttcacca acaaaaaaaa ggtttacacc
     8521 atccccttgc cctggtatct taccatattt acaccacact ttttttgtat ctggatcatt
     8581 ctttgatacg attttcgcca cttgttccct ggccctcact gtcaatgt
//
LOCUS       DAZKGS010000177         8508 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKGS010000177 DAZKGS010000000
VERSION     DAZKGS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..272)
                     /gene="cas2e"
                     /locus_tag="ACHOQY_004135"
     CDS             complement(<1..272)
                     /gene="cas2e"
                     /locus_tag="ACHOQY_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9680120.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVS"
     gene            complement(272..1192)
                     /gene="cas1e"
                     /locus_tag="ACHOQY_004136"
     CDS             complement(272..1192)
                     /gene="cas1e"
                     /locus_tag="ACHOQY_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9680121.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(1189..1839)
                     /gene="cas6e"
                     /locus_tag="ACHOQY_004137"
     CDS             complement(1189..1839)
                     /gene="cas6e"
                     /locus_tag="ACHOQY_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP9680122.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(1821..2567)
                     /gene="cas5e"
                     /locus_tag="ACHOQY_004138"
     CDS             complement(1821..2567)
                     /gene="cas5e"
                     /locus_tag="ACHOQY_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9680123.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(2578..3633)
                     /gene="cas7e"
                     /locus_tag="ACHOQY_004139"
     CDS             complement(2578..3633)
                     /gene="cas7e"
                     /locus_tag="ACHOQY_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9680124.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(3696..4184)
                     /gene="casB"
                     /locus_tag="ACHOQY_004140"
                     /gene_synonym="cse2"
     CDS             complement(3696..4184)
                     /gene="casB"
                     /locus_tag="ACHOQY_004140"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9680125.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IR"
     gene            complement(4181..5743)
                     /gene="casA"
                     /locus_tag="ACHOQY_004141"
                     /gene_synonym="cse1"
     CDS             complement(4181..5743)
                     /gene="casA"
                     /locus_tag="ACHOQY_004141"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9680126.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(5841..>8508)
                     /gene="cas3"
                     /locus_tag="ACHOQY_004142"
     CDS             complement(5841..>8508)
                     /gene="cas3"
                     /locus_tag="ACHOQY_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP9680127.1"
                     /translation="KNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKR
                     NCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKN
                     YEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMD
                     ELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADW
                     TGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPT
                     PLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMAT
                     ANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFS
                     QGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACD
                     AYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDY
                     PWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSV
                     DDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIV
                     CTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELL
                     ILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESV
                     YGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAE
                     ESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIE
                     QRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         2031 a         2363 c         2020 g         2094 t
ORIGIN      
        1 aaagaaacca aacgtaaccc atccaaatcc accggaatac gtctgttttc tccccaggtc
       61 tgaaattcaa aacccgactc ggtattggtc gcccaggcca tcaccacatt tccgcaacca
      121 gccagttggg taatttgctg ccagatcatc tcccgaatac gttttgatgt atcaccaaca
      181 tacacaccgg cacgcacttc cagtagccag attgcgagcc gtccacgtaa gcgcggcggg
      241 acattttctg taacaaccac gaccatgctc atcagccgcg tcctcgatga ccgctatcgc
      301 ccaacgtttc aggttcgggg atggctggcg gtaacatatc cggcgcaggt tgtggcggtt
      361 caatttcacc tgcggcaagg acttcctcaa ttaacggtat taatttgccc gttaacttag
      421 tgctacggaa aatatcgcga caggctaatc tgacttcttt atcaggttct gcgggttgcc
      481 tcgctgctat ttcaaatgcc tttggcacaa ccgaatcaaa tttaatgata tcggctatgt
      541 cataaacaaa tgaaagcggt ttgccactat ggataaaccc aatagcgggc gcatatcccg
      601 cggctaatac tgccgcttca gatataccgt acagacatga tgtagcagca ctgatgcagc
      661 gattcacaac atcgcctttt tcccagtctt taggatcgta tttgcgacca ttccatttca
      721 caccatattg tttcgccagt aatgcatacg tctggcgaac gcgagatccc tcaattccac
      781 gtaactgctc cactgaacgg cgagctggct gtggttcacg aaaacgtaat tcatacattt
      841 tgcgcaccac cttcaaccgt agatcttccg ttaaagccag ctttgcctgg tagagtaatt
      901 tatctgcccg cgccccaccg ggttgtccgg aggagtaaac gcgaacgccc gcttcaccga
      961 cccagaccag cagtgttccc accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg
     1021 ttcctggttc gagcataatg caggcgaccg atcccaccgg aatatgcgtt cggatccctg
     1081 ttttgtcgat cagcacgaaa gcgccgtcca gtacgtcgat ctgaccgtac tggaggaaga
     1141 tcatggaggt gcgatctttt aatgggatcg ggctcagtgg tacaaacgtc acgcctctgc
     1201 tccgggtttg atcagcatca gaccacagcc gaacgcccgg cttttaccat acccctggct
     1261 caggcgttgc acaaataacc caggatcggt gaccgtaagc atccccgtat aatccacgct
     1321 actgaactgg atcagttgcc gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc
     1381 aaccgaggta tccagcagtg taaaaccgct tctctccccc tgcgctgcca gccagtccag
     1441 cgccgcctgt tgttgatgca accagacatc acttccttcc gccggccccc tcacctgccg
     1501 tttcgcctcc atcagcagat cgtggcgctt gcccgcttta cagattgttg gattcgcccg
     1561 caggttgaaa caaagttgtt gtccggtaca cagctcgggc gcaaatgacc ggcattctat
     1621 ggtgaacgta tcactttccg ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc
     1681 ctggagttct tcccgacgat aaagaaattg cctttctttg ccgccaggga agagatccca
     1741 cagccactga tgcatcacat attcaccgcg atccaccaga tgcagcaact gcgcaggcga
     1801 aagctggccg gtatgtaagg ttattcttga gaggtacatg gttcctcctt gctgagccac
     1861 ggcccctgat tgacggtgcg ttccccaaac agccactgct gacgatttaa aggaacatcc
     1921 cggcggcgta atattttgct cgcgaccagg ccgtcgtgtt tcccttccca ccagcattca
     1981 tcctgaagtt tcgggagtga gactttcagt ttatggaaat gatcctgata ctgttggtat
     2041 gcattacata aggcatcaca cgcgttgcct tcgagcagta acggcgcaag aggtaatgcc
     2101 agcggatgac tttttcgccc cagataaagc ggaaaaactg gatggcgtaa accgtcctgc
     2161 aactgttcga ggctgtaagg cgcatcggct gttgttgcca ccgccaccat ccaccaggca
     2221 tcggtgtagt agtcgcgccg ggagataatc gcgctcaaaa gctcagggtc gctcaactct
     2281 tcgcgacggc tgaaataacg tgctttacgc acctcttttg gcatctggac cgtgtgataa
     2341 tcccgtgccc agcgcgggtt acggctggcg caaaccacca gtgaatagtg gcgattaaac
     2401 gcgtttaagc gttcggtatc atcacgccga atccctaccc cggcagccag tagccccagc
     2461 aatgctgagc gcgaaggaag ttcatgggta tgacgaactt cacctggagc atcgcccccc
     2521 caggacgcca tcggcccatg aagctgaaaa atcagatatt ggctcataag ccgcccctta
     2581 cgcgcagata aagtccagca cgtccttcat gcttccctgt ttgttcatca cgtcaaaact
     2641 tgcgcattcg gtcttctgtt cgtagaccgt attcatgttt tcgcgaagcg ttgtaatacg
     2701 ctgcaccgcc acatctaact gacgtgtgcc attaatgggt tcatagaaag ccgccgccag
     2761 agagcgtggt tgatcggtgc ctttttctgc cagcgcccag gaggcgtagg cacggctggc
     2821 aaagctgttc tgtttgccgg ttggggagac tttaagtgcg gcttccgtaa aggcacgtat
     2881 ggtctgatta gctaacgctt cgtcaccgcc aaggttttcg accagcagat ctttatcaat
     2941 gcagatatag gtgtagaaca gtgcagaacc gaatccggtt tcaccaagat gcccggcacc
     3001 ggcatcttca gaagcctggc gcaaatcatc aacggcggtg aaaaaatcat cttcgacaat
     3061 cgtttcactg acaccaaatg cgtgcgctac ctggcaggcg gcttcaacat taaactcggg
     3121 tttattcgcc agcatacgac caaacatggc aatatctacc gccatacggt ctttgcgtaa
     3181 caacgcgaga tcttcctctt ttggcgcgcg cttttcttct gcaagctgat gggccagcgc
     3241 ttttacggcg tcaaattctg ccgggctgat atggactaac tgttcagttt cggcgttagt
     3301 gagcggatct tttggttttt tgtcattttt agctttccca agataatcag caatttttgc
     3361 cgaccattcg atggcttttt tatcttcaat ccctttctca atcaggatag ttgccgcctc
     3421 acgcgcaata cgcccactgc gaataccaat atggcccgcc agtgcctgtt caaaaagtgc
     3481 agaagtgcgc cacgcacgtt tcagactttg cgaggaaacg cgcagacgcg ttgctccacc
     3541 cagcaccaca gtttttggcg caccggtatc atcacggtta aggttggcag cagggtaagc
     3601 ggttaacaaa tgaagctgaa taaatgttgt catgagatag tcctttattg attttgttcg
     3661 ttatcggcgt cgccagcctg gtaatattcc agcgctcagc ggatacggat aaattcggtt
     3721 ggccgctgct ggcggcggtg atgattgagc aggtcgtcgc tctcctggca ccaacggaag
     3781 accccctctg ccagagagtc aagattgacg gaaccgttta ataatctgac tgcgcggcgt
     3841 aactggcgca gtaattcatc cggtgttttt actgccgaca gacgagtaaa acgccccttt
     3901 gacattaccg ccgccagctg cgcggcaaaa ggctgccgtt catcaatagc ttttacattg
     3961 gcgctaactg cggcgaccag cgccagtgcg gtaaaacgcc actcttgtgc ttcaattttc
     4021 cacaaagtgt gggtctgcat cagcagagaa cgaaaccctt ctgacaagca aacatcatta
     4081 accgttgtgc tacgccgcag acttgcacga ttgccgcgtt tagcctgcaa ctcttcgcgc
     4141 catttacgca gcgtggcttt gtgctcctct tttacaatac tcattcagca gcctcctgct
     4201 ttttctccct ggcggcttta gcactttgct tctccgccga tgttgtgaaa tatttcttgc
     4261 gcgcggtcat aacgcgtttc aaatcaacag gctcatatgg attggtgaat acacgctcgt
     4321 caaaatcctg acgcgcgaat aaccaaattt ccttttgcca tttgccgagt aattcgtccg
     4381 catcctgacc ttcttcaatt tggcgcacta acctcaggaa gcgatgctga gttttgttcc
     4441 agaagtcgat atccacaaaa ctgaaatcac cccgtgcacc ttttggatcg gagaaccacg
     4501 cttctttcaa tgcacttcgt aacagactta gaatccgtga agccgtttgc gcagccagac
     4561 gcagtttcgg tatctggcct tcttttttct tgagcagcag cgggaaatgg tgttcgtacc
     4621 aacagcgcgc tttcatgttg tcgaaatcat agccaaatcc ccacaggcct acttttgcct
     4681 gtttcagact gctggcatta aagagtttca ccaccagcgc gggaagttcc gtattgtttt
     4741 ctgatttacc cgtttcgata aggcctaacc agtcgcgcca gattaaaccg cccggttgtg
     4801 gtttgaccga gtaaaactcg ccaccctctt taagtggtat acggtaaggc gttaagggat
     4861 gctgccacat ggcataattc gcaccgtaat ttttggtcgt catcaggctc aggagcgcgt
     4921 cgctctgttc accgcaaata tcgcagttgc cgactgtcgt ggtattaaaa tcaatacgaa
     4981 tacggcgcgg cattccccag taagcctgga gtttattgac ctgatcatgg gttaccaccg
     5041 caccggccag ttcgctggtt cgcgtcgggc caagccaggg gaaaaccaga tcgtcaaatt
     5101 ttttgggtag cggtaagtcg gcttcatcct gcggcatcac attgggccac agtttgcgcc
     5161 acaagggcgt ttgttgattg ccttgatatt cctgtaattc aatcagagtg gtcatcggcc
     5221 caccgccgcg caaaccggtg cgatagcctt tgccgcctga tggcgcattt aactgtaggg
     5281 agaacagagc taacgcggaa caatgagggc atacgtgttc agtcacgcca cgcttaataa
     5341 agtggtcttt attaaacttc gttgtttgag cgccgggaat ctcaggcagt agcgaagcga
     5401 cctgaacttt atctcccttg agcgcctcga aatcctgcat aaatgaaggg gaatctgccc
     5461 caaactggaa agcgtgttct aaggacagca atgcttcccg tagcttttca gcctccagtc
     5521 cgtcttgcca gatatcatcc caacgaccgt gatttttggg cgcgaagctg gtttgtagta
     5581 gccccagcaa aaactgccag gccgccccct ggagatctgc ccgcggcgca gcgatatcga
     5641 caacattttc atcagccaga tcgactggtg ccagcttgcc tgtcgttccg tctttaaaac
     5701 gaacaggcaa ccacggggtt gtcagaagtg aaaacgagtt catacgcatc ctctcgtaaa
     5761 aaagaccatc ccaggtcggt tgtccatctt tccatccttt tcggtgtatc aactattcag
     5821 agagtcctgt cgcagacatc tcaaccaacc ttgccaatca atccttccgt agccgaatac
     5881 ccgcaacttt cgtcatcgtt gactaaaatc acgtttgcca tctccggatc ttgtcgctgt
     5941 tcaatgcacc acagcctgaa cgcctctccc tccagtaaag aaaactcatc ccgatgtttt
     6001 ttccaccagc ttcggcgcac tctgacaacg ctcatttccc atgcgtgagc accggtggca
     6061 taaggcttca ccacaccgtc aatacaggta gccagccaca gggaaacaga ctcctcagcc
     6121 agacgtgtcg acagtttttc cgggaggtaa tcgttgatat tggcggcata gccgggcctg
     6181 aagttcagca caaacttttt agccattgcg cgatcgcagt aatatttgcc cacttgctcc
     6241 tgctcgctgc gggcaaatcc ttccggcatt accacgtcct caccgtagac ggattcaata
     6301 agaagccggg ctgcgtgtgg catctgaata gcaccttgct cacgcagcac acgctgcgtc
     6361 agccagattc gcccatgatc gggatagaca aatgcactgt tacgcatggc actgccaaac
     6421 cattcgtcgc caggagagtc gtcccagacg ggagccagaa tcagcaattc agggggggaa
     6481 cgctcgtcct ttccgtcacg ctttaactga ccattaatat cgcggatatg ccgctgtaat
     6541 cgtcctgctc gctgaatcag caaatcaaca ggggccaggt cggagatcat ttcgtccagg
     6601 tcaatatcca cgctttgctc aattacttgt gtacaaacaa tgacttgcga cgctctctgt
     6661 aaactacaat atttaccaaa gcgtgccagc gtttccgttt caattcgctg gcgatcgcta
     6721 aaagcaaagc ggctatgaaa aagtgaaagg ctggaagcag gaatgacgcc gcgggcaagc
     6781 agctgacgat gaaccttaat agcgtcatcg acagaattcc ggatccaggc gatgcatttc
     6841 ccctgactta ccgccgattc gatacgcgca atacactctt gttcactatg aagccaaccc
     6901 acgctgacgc tacgctcaac atctttgcgc gtcgctaccc ggtgtgagtt cacatcggat
     6961 ttcgtgacat gcgtcagcca ggggtaatca tccttttcca ggaacggagc ttcttgctgg
     7021 ccctctgtac cccgcgcaaa ggcagcgacg agtttgtcgc gctgctgttg agataacgtg
     7081 gcagaaagca aaataacgct gtttccgcca cgcgcctgcc gctcgatcaa cccttcaaga
     7141 atgcacgaca tgtaagcgtc acaggcgtgg atctcatcag ccagcaggat tttgttactc
     7201 agccccagaa gccgcagatt attatgtttg aacggcatca ccgccatcat cgcctgatcc
     7261 agcgtgccga cgccaatttc agccagcaac gccttcttgt tactgtcggc aaaccaggcc
     7321 gcacatccct gactgaacgt ttgttcatcc ggttcttctg acccgactaa atcaccagac
     7381 cagagtgatt cattaaagcg gtccattaat gtgcgggcac tatgtgccag caccaggctg
     7441 gggcgggatt ctggcgaata gaaagcaagc caggttttga ccagccgatc gtacatggca
     7501 ttggccgttg ccattgttgg cagaccaaaa aacagcccct gagctttccc cgcagctatc
     7561 aacctgtgcg ccaggataag cgccgcttct gttttacctg cgccagtcac atcttccaga
     7621 ataaataact gcggtccttg ctggctgata tccagatcca gcaccttttg ctgtaacggt
     7681 gtcggattct caataaaagg aaacagcgta ttaattccgt tgaaaggcgc gacttttgct
     7741 tttagaggaa agacggttaa ggcgttttga gcctgaatta aagttttctg ccagtaacct
     7801 ttaatatcca ttggatgtgc gacgcgtgga aaaaatcgcg ttgacgaacc cgtccagtct
     7861 gcgagcacga cggttgcaga gatataccag gaaaggtgtt ttataagttc aatgccctca
     7921 tcatcatccc agaatgcggg gatctctatg agcggaaaca gtggtttgat ttcaaggaga
     7981 aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca
     8041 gggggctgac catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt
     8101 ttgcgcggag agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc
     8161 cacaaccagt agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc
     8221 tgaatcttga gttcaggggg caggtaaagt tgttgaaaag agcgggcaaa ttttccaata
     8281 tcgtgccagc acagcaacca ggcgaaaaat tgagccgcct gttccttgtc agaaatccct
     8341 aattgacgaa aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatc
     8401 gcggctacat ccagcgaatg ccagcaaaga aggtgatagt cgtcgccccc gtctttctcg
     8461 ccgcgtcggg tttttcccca gaaatcaaaa aaagtcacaa tattttta
//
LOCUS       DAZKGS010000178         8462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKGS010000178 DAZKGS010000000
VERSION     DAZKGS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..76
                     /gene="yobI"
                     /locus_tag="ACHOQY_004143"
     CDS             11..76
                     /gene="yobI"
                     /locus_tag="ACHOQY_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP9680128.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            215..826
                     /gene="ruvA"
                     /locus_tag="ACHOQY_004144"
     CDS             215..826
                     /gene="ruvA"
                     /locus_tag="ACHOQY_004144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9680129.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            835..1845
                     /gene="ruvB"
                     /locus_tag="ACHOQY_004145"
     CDS             835..1845
                     /gene="ruvB"
                     /locus_tag="ACHOQY_004145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9680130.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1992..2777)
                     /gene="znuB"
                     /locus_tag="ACHOQY_004146"
     CDS             complement(1992..2777)
                     /gene="znuB"
                     /locus_tag="ACHOQY_004146"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9680131.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2774..3529)
                     /gene="znuC"
                     /locus_tag="ACHOQY_004147"
     CDS             complement(2774..3529)
                     /gene="znuC"
                     /locus_tag="ACHOQY_004147"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9680132.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3608..4540
                     /gene="znuA"
                     /locus_tag="ACHOQY_004148"
     CDS             3608..4540
                     /gene="znuA"
                     /locus_tag="ACHOQY_004148"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9680133.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4556..5878
                     /gene="mepM"
                     /locus_tag="ACHOQY_004149"
     CDS             4556..5878
                     /gene="mepM"
                     /locus_tag="ACHOQY_004149"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9680134.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQVVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5998..6969
                     /gene="lpxM"
                     /locus_tag="ACHOQY_004150"
                     /gene_synonym="msbB"
     CDS             5998..6969
                     /gene="lpxM"
                     /locus_tag="ACHOQY_004150"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9680135.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7058..>8462)
                     /locus_tag="ACHOQY_004151"
     CDS             complement(7058..>8462)
                     /locus_tag="ACHOQY_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFP9680136.1"
                     /translation="FDEIWKAMLTLQFHDILPGSCISRVYHETEKEYLKLEAKTEKII
                     SDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAVIDPKNK
                     IVNGLKAESRSIENNYIKLLFSESGDLISLYDKRYGKEYITENMHSEIRAYHEDAGFF
                     AAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVD
                     VQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHW
                     VDLSDQETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEVVKTDNYDNFLINNFADI
                     GKQKFTFSLFPHHGDYRVGGVVNQAYIMNHPLQIVPVKPHPGELPPSLSFFAIDTNSV
                     IIETIKLAEDGDGIIVRLYESYGLEISAMFSWVCNYHYVQYVDLQENKLDDSFYCNQY
                     CNLEFKPFEIITLRLTQ"
BASE COUNT         2266 a         1980 c         2102 g         2114 t
ORIGIN      
        1 aggagtcgca atgtatattt ttattacgca tttcttcact gaatatgtaa tattcaaata
       61 tttgcttcca atataacctg tagaataaat tatactgcgc catttttcag atcgttggga
      121 cgcctcgcaa gttttcttca tccttcgctg gatatctatc cagcattttt ttatcataca
      181 gcattatctt tgattcatta cgcaggagcg tcatgtgata ggcagactca gaggcatcat
      241 cattgaaaaa caacccccgc tggtgttaat tgaagtgggc ggcgtaggct atgaagtgca
      301 tatgccgatg acctgttttt atgaactccc tgaagcgggt caggaagcga tcgttttcac
      361 ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt tttaacaata aacaagagcg
      421 cacattgttc aaagagttga tcaaaaccaa cggcgtcggc ccgaagttgg cgctggcgat
      481 cctctccgga atgtcagcgc agcagttcgt taatgccgtt gagcgtgaag aagtgggggc
      541 actggtgaaa ctgccgggta ttggcaaaaa aaccgccgaa cgcttgattg ttgaaatgaa
      601 agaccgattt aaaggtttgc atggcgatct ctttacgcca gccgccgacc tggtactcac
      661 gtcacctgcc agcccgacga ccgacgatgc tgaacaggaa gcggttgccg cgctggtggc
      721 gctgggctat aaaccacaag aagcaagccg catggtgagc aaaatcgctc gccctgacgc
      781 cagcagtgaa acattaattc gcgaagccct acgcgccgcg ttatgaggta aaggatgatt
      841 gaagcagacc gtctgatttc tgccggtacc actttgccgg aagatgtggc agatcgcgcc
      901 attcgcccta aattactgga agagtatgtt ggtcagccgc aggttcgttc acagatggag
      961 attttcatca aagcagcgaa actgcgcggc gatgccctcg atcatctgtt gattttcggt
     1021 cctccggggt tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat
     1081 ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg atctggcagc gatgctcact
     1141 aaccttgaac cgcatgacgt gttgtttatt gatgagatcc accgtctttc gccagtggtg
     1201 gaagaggtgt tgtatccggc aatggaagac taccaactgg atatcatgat tggtgaaggt
     1261 ccggcggcac gctccattaa aattgatttg ccgccgttta ccctgattgg tgcaaccacg
     1321 cgcgcaggtt cgctgacatc accgttgcgc gatcgttttg gtattgtgca gcgtctggag
     1381 ttttatcagg tgccggatct gcaatatatc gtcagtcgca gcgcacgctt tatggggctt
     1441 gagatgagtg atgacggcgc gctggaagtt gctcgtcgag ctcgcggtac gccgcgcatt
     1501 gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc
     1561 tcggcagata tcgctgctca ggcgttggat atgttgaatg tcgatgctga aggtttcgat
     1621 tatatggacc gcaagttatt gctggcggta atcgataagt tctttggtgg gccggtaggt
     1681 ctggataacc ttgcggcagc cattggcgaa gaacgtgaaa ccattgagga tgtgctggaa
     1741 ccttatttga ttcagcaagg ctttttgcag cgtacaccgc gtgggcgtat ggcgacgacg
     1801 cgggcgtgga atcactttgg cataacgccg ccagaaatgc cgtaagtcgg attggtcgct
     1861 cgcgccgcat ccgacactgg cagtgtgtct gatgcgacac tgttcgcgtc ttatcaggcc
     1921 agccagagat agcgaaccgt aggtcggata aggcgctcgc gccgcatccg acaaatgtgt
     1981 tcagcgatag attagctggc ctgctttttc atcatactga gaataaataa cagtgccgca
     2041 catagcacca ccgacggacc cgccggcgta tcgtaaaacg cggaaaaggt taaaccgcca
     2101 gttactgcca ccatccccac caaaacagcg acaccagcca tctgttccgg cgtacgggca
     2161 aagcgacgcg cagtagctgc aggaataatc agcagtgaag taataatcaa cgcgccaaca
     2221 aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca tcaacaacaa tttcacgcgc
     2281 tgtaatttca caccatcaac aaacgccaga tccgggctaa tcgtcatcga cagcaaattg
     2341 cgccattgcc agaacaaaat agccaccacg atgaccacgc caatcgcaat agcgatgaga
     2401 tcttctggcg tcactgccag caaatcaccg aacaggtaag ccatcaaatc aacacgaatg
     2461 ttagacatca gactaacgac caccaggccc agcgacaggg cactgtgcgc cataatccct
     2521 aataacgtgt caatcgccag ctgtggacgc ttctccagcc ataccagacc gcccgccagg
     2581 agcagcgtaa cggcaattac cgcatagaat ggattcacgt ccagcaacaa accaaacgcg
     2641 acgccaagta atgaggcatg agccagcgta tcaccgaaat aagacatacg acgccagact
     2701 acaaacgaac ccagcggacc cgcggcacag gcgagcatga tcccggctaa ccaaccggga
     2761 aataataatt caatcatgag cgatcatttc cccgacgcaa aacaattcgt ccctgtaaat
     2821 cgtgacgatg attatgatga tggcgataga tacccagttg ttcagcacca cgaggaccaa
     2881 acatagaaat aaactccgga tgtagggaaa caacttccgg tgtgccggaa caacaaatgt
     2941 ggtgattcag acacagcact tcatcggttt ttgccattac cagatgcaga tcgtgagaga
     3001 ccattaaaac gccacaatcc agttcgcgac gcaactggtc aataaggtca tataacgcca
     3061 cctggccatt tacatccacg ccttgagtgg gttcatccag cactaataat tgcggtcgat
     3121 ttaacaatgc tcgcgctaac agtacacgct gtgtttcgcc accagagagc ttttgcatcg
     3181 gtgcgttaat cagatgcccg gcctggacac gtttcagtgc aggcaaaata tcttctttat
     3241 gtgtgccagg gcgtaagcgt aaaaaacggt ttacggtcag tggcaacgtg gtgtcgagat
     3301 acagcttctg cggtacatag ccgatgcgta gttttccgtt gcgcttgata accccttcat
     3361 cgggtgttac cagcccgagc actacccgta ccagtgtcga cttacctgcg ccatttggcc
     3421 caagtaaagt caaaattttt ccaggtttaa gttccagcga cacatcagag aggacgcggc
     3481 gttggccaaa agaaaccgag acattttcca gggaaaccag acttgtcatg ttaattttag
     3541 tcttgcagta gtcatgaaat gttataatat cacacttctc atattcatta cgatgattgg
     3601 tcgcattatg ttacataaaa aaacgcttct tttcgcagca ttatccgccg ctctctgggg
     3661 aggtgcaaca caggccgcag atgccgccgt tgtcgcttcg cttaaacccg ttgggttcat
     3721 cgcttctgcc attgctgatg gtgtaacaga aacggaggtt ttactgcctg acggcgcttc
     3781 agaacatgat tattcactgc gtccatcgga tgtaaaacgc ttacagaacg cggacttagt
     3841 cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa ccggtaagca aattaccagg
     3901 agcgaagcag gtaacgattg cgcagcttga agatgtgaaa ccgctactga tgaaaagtat
     3961 tcacggcgat gatgatgatc acgaccacgc ggaaaaaagt gacgaagatc accatcacgg
     4021 cgatttcaac atgcatcttt ggctttcccc agagatagcg cgggctacag cggttgcaat
     4081 ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc aaacttgacg ccaacctgaa
     4141 ggattttgag gcacaattag cctcaaccga aacgcaggtt ggtaacgagc tcgcgccgct
     4201 caaggggaaa ggttatttcg tttttcacga tgcttacggc tatttcgaaa aacagtttgg
     4261 actgacaccg cttggtcatt ttaccgttaa ccctgagatt caacctggcg cgcagcgttt
     4321 acatgaaata agaacacagt tggttgagca aaaagcaacc tgcgtttttg ctgagccaca
     4381 gttcaggcca gcggtcgttg agagcgtcgc acgagggaca tccgttcgta tgggaacgtt
     4441 ggatcccctg gggacgaata tcaaactggg taaaacaagc tattcagaat tcctgagtca
     4501 attagccaac cagtatgcga gctgcctgaa aggagattaa tgaggaagtg attacgtgca
     4561 acagatagcc cgctctgtcg ccctggcgtt taataattta ccgcgaccac accgcgttat
     4621 gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc tggcggcctt atgtttatca
     4681 ccgtgatgcc acgccaattg tcaaaaccat tgagctggaa cagaacgaaa ttcgttcgct
     4741 cttacctgaa gccagtgagc cgattgatca agctacacaa gaagatgaag ccattcccca
     4801 ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg catgaatatg ttgtttccac
     4861 tggcgatacg ctaagcagca ttctcaatca gtatggtatt gatatgggtg atatcaccca
     4921 actggctgcg gccgacaaag aattgcgtaa cctgaaaatc ggtcaacaac tctcctggac
     4981 attaaccgcg gacggcgaac tgcagcgcct cacctgggaa gtgtctcgtc gtgaaacccg
     5041 aacctatgac cgtactgccg ctaacggttt taaaatgacc agcgaaatgc agcaaggaga
     5101 gtgggttaac aatctgctga aaggtaccgt cggggggagc tttgttgcca gcgccagaaa
     5161 cgccggttta accagcgccg aagtgagcgc agtgattaaa gccatgcagt ggcaaatgga
     5221 tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtgtta atgtctcgag aaatgcttga
     5281 tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg cgttcagaag gtaaagatta
     5341 ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt aacggtactg gtctggcgaa
     5401 aggattcttg cgattcccga cggcgaaaca gttccgtatc tcgtctaact ttaacccgcg
     5461 tcgaactaat ccggtgacag gtcgcgttgc accacacaga ggtgttgatt tcgccatgcc
     5521 acaaggtacg ccagtgcttt cagtgggtga cggtgaagtg gtggttgcca aacgcagtgg
     5581 cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc tacaccacgc gttatatgca
     5641 cttgcgcaag attctggtga aaccgggaca gaaggtgaaa cgtggcgacc gtatcgcgct
     5701 ttccggtaat accggacgtt caactgggcc gcatctgcac tatgaagtat ggataaacca
     5761 gcaggtcgta aacccgctga cggcaaaact gccgcgtact gaagggctga ccggctccga
     5821 tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg cagctacggt ttgattaatt
     5881 aacatccatt cgcagccggt acgcagtcag taccggcttt ttttatttgg tgcggggcaa
     5941 gttgcgccgc tacactatca ccagattgat ttttgcctta tccgaaactg gaaaagcatg
     6001 gaaacgaaaa aaaataatag cgaatacatt cctgagtttg ataaatcctt tcgccacccg
     6061 cgctactggg gagcatggct gggtgtagca gcgatggcgg gtatcgcttt aacgccgcca
     6121 aagttccgtg atcccattct ggcacggctg ggacgttttg ccggacgact gggaaaaagc
     6181 tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc cagaacgtag tgaagctgaa
     6241 cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc aagcgatggc aatgatggct
     6301 gagttggcaa tacgcggacc ggagaaaatt cagccgcgcg ttgactggca agggctggag
     6361 atcatcgaag agatgcggcg taataacgag aaagttatct ttctggtgcc gcacggttgg
     6421 gccgtcgata ttcctgccat gctgatggcc tcgcaagggc agaaaatggc agcgatgttc
     6481 cataatcagg gcaacccggt ttttgattat gtctggaaca cggtgcgtcg tcgctttggc
     6541 ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca tccagtcggt acgtcagggg
     6601 tactggggat attatttacc cgatcaggat catggcccag agcacagcga atttgtggat
     6661 ttctttgcca cctataaagc gacgttgccc gcgattggtc gtttgatgaa agtgtgccgt
     6721 gcgcgcgttg taccgctgtt tccgatttat gatggcaaga cgcatcgtct gacgattcag
     6781 gtgcgcccac cgatggatga tctgttagag gcggatgatc atacgattgc gcggcggatg
     6841 aatgaagaag tcgagatttt tgttggtccg cgaccagaac aatacacctg gatactaaaa
     6901 ttgctgaaaa ctcgcaaacc gggcgaaatc cagccgtata agcgcaaaga tctttatccc
     6961 atcaaataaa aaacagcctc tcaaattatt gagaggctgt acttgataaa aaatctcaat
     7021 gagtgttctc accattttga gtttttagat tagtacgcta ttgagttaat cgtagcgtga
     7081 taatttcaaa aggtttgaat tcaagattgc aatattgatt gcaataaaaa ctgtcatcta
     7141 atttattttc ttggagatcg acatattgaa cgtagtgata attacatacc cacgaaaaca
     7201 tggctgaaat ttctaatcca taactctcat ataatcttac gattataccg tcgccgtctt
     7261 cagctaattt tattgtttcg atgataacgc tgttagtatc gatagcaaaa aatgatagtg
     7321 aagggggtag ttcacccggg tgtggtttga cagggacgat ctgtaaggga tgattcatta
     7381 tataggcctg gttaacgaca ccacccacac ggtaatctcc atgatgagga aataaggaga
     7441 atgtgaattt ttgtttacca atatctgcaa aattattaat caggaaatta tcataattat
     7501 ctgtcttaac aacttcccca ggtttgtgct gcgaacgcaa caaagttaac tctagcgtct
     7561 ggtctttgac acgatagcca tatttgtttt ttgctattaa agcaatgcca gtttcttgat
     7621 cagaaaggtc gacccagtga tgtgctggta tttcatcttt ggcaaaagcg aacgaatcat
     7681 cgctatgcgt tggtctgtca attacaccga actgaatttg acattgtgca agagatgttt
     7741 gtactgatac cgggaatttt actttgagag aaacattagg ttcgtgccaa tcaataaaag
     7801 tttggacatc aacgcgtgga ctgtcctgtg tcagggtaaa tgcgaagcga agataagagg
     7861 aattgtagtg ataaatcagc gtcatcgtag ttttaggccc gctgatcaca gttgtcattt
     7921 tttcagcgag taaaacatat gattccccat cacgataatt tgaagcaaaa tcccaggccg
     7981 cgaaaaaacc tgcatcttcg tggtatgcac ggatctcact gtgcatattt tcagtaatgt
     8041 attctttacc atatctttta tcatatagcg aaattaaatc accgctttct gaaaaaagaa
     8101 gtttaatgta attattttca atacttcgag attctgcctt cagaccattt acgatcttat
     8161 tctttggatc aatcacagca taaccataag agtttactcg tgctttgagc cagttgttgt
     8221 ttatattgat ccattcgtta cgagcaaaac aggtggtatt aaataaaatg tgcggatctt
     8281 tataagatga agtgtcaatt ttacttaata aagtagactg cgcatcagaa ataattttct
     8341 cggtttttgc ttctagttta agatattcct tttcagtttc atgataaact cttgaaatac
     8401 atgagccggg aaggatgtca tggaattgca aggtcagcat cgctttccat atttcatcaa
     8461 ac
//
LOCUS       DAZKGS010000179         8444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKGS010000179 DAZKGS010000000
VERSION     DAZKGS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHOQY_004152"
     CDS             <1..224
                     /locus_tag="ACHOQY_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680137.1"
                     /translation="PVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETL
                     FSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            447..944
                     /gene="ubiC"
                     /locus_tag="ACHOQY_004153"
     CDS             447..944
                     /gene="ubiC"
                     /locus_tag="ACHOQY_004153"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9680138.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            957..1829
                     /gene="ubiA"
                     /locus_tag="ACHOQY_004154"
     CDS             957..1829
                     /gene="ubiA"
                     /locus_tag="ACHOQY_004154"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9680139.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1984..4407)
                     /gene="plsB"
                     /locus_tag="ACHOQY_004155"
     CDS             complement(1984..4407)
                     /gene="plsB"
                     /locus_tag="ACHOQY_004155"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9680140.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4578..4946
                     /gene="dgkA"
                     /locus_tag="ACHOQY_004156"
     CDS             4578..4946
                     /gene="dgkA"
                     /locus_tag="ACHOQY_004156"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9680141.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5056..5664
                     /gene="lexA"
                     /locus_tag="ACHOQY_004157"
     CDS             5056..5664
                     /gene="lexA"
                     /locus_tag="ACHOQY_004157"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9680142.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5737..7062
                     /gene="dinF"
                     /locus_tag="ACHOQY_004158"
     CDS             5737..7062
                     /gene="dinF"
                     /locus_tag="ACHOQY_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9680143.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7178..7387
                     /locus_tag="ACHOQY_004159"
     CDS             7178..7387
                     /locus_tag="ACHOQY_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9680144.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7429..7944)
                     /gene="zur"
                     /locus_tag="ACHOQY_004160"
     CDS             complement(7429..7944)
                     /gene="zur"
                     /locus_tag="ACHOQY_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9680145.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8262..>8444
                     /gene="yjbL"
                     /locus_tag="ACHOQY_004161"
     CDS             8262..>8444
                     /gene="yjbL"
                     /locus_tag="ACHOQY_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFP9680146.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRYRDIII"
BASE COUNT         1957 a         2023 c         2291 g         2173 t
ORIGIN      
        1 ggccagttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata
       61 ccaattggaa ttccacgtgt atctataaat ccagacataa gatgttgcct attaatctta
      121 ctcaggaaac actttttagc tccaaatctc atggtaaata tgcgcttttc ccaattttta
      181 ctgcgagttg gcgagctact cgtataaaga atataggtat ttaagtaaag gaaaacatca
      241 ccgttcctgg catcatgggc ggtgatgccc ctacggttgc cctcgccagc acgggcatcg
      301 gcaaagcgta aggttcaaca gcggtttacc acttcatgcg attgttgcgt ttttgttgcg
      361 cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct
      421 ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc
      481 tgcgctattt taaagagatc cctgccctgg atccgcaact gctcgactgg ctgttgctgg
      541 aggattccat gacaaaacgt tttgaacagc agggaaaaac ggtaagcgtg acgatgatcc
      601 gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg ccgaaagagt
      661 ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc
      721 gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa aaattgggta
      781 aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac tttattgaga
      841 taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc
      901 tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg
      961 agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc
     1021 caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg
     1081 gcgttcctca gctctggatc ctggcggtat ttgtcgcggg tgtctggctg atgcgcgctg
     1141 ctggatgtgt ggtgaatgat tatgctgacc gaaagtttga tggtcatgtt aagcgcacgg
     1201 cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgca ctgtttgtag
     1261 tgctggtact gatttcgttt ttactggtac tgacgctgaa tacgatgacc attctgttgt
     1321 cgattgccgc tctggcgttg gcgtgggtgt acccgtttat gaagcggtat acccatctcc
     1381 cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gccgctgtga
     1441 gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg
     1501 cttacgacac gcagtatgcg atggttgacc gcgatgatga tgtgaagatt ggcattaaat
     1561 ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg cagattggcg
     1621 tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga tattactggt
     1681 caattgtggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc
     1741 gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta ctatttttag
     1801 ggctggcaat gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct
     1861 ttcttctggg ttgcctgatg cgcggcgctt ctcaggccta cacaacacat cgcaatttat
     1921 tgaatttgca gattatggaa ggccggataa ggcgttttcg ccgcatccgg caattctctc
     1981 tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa
     2041 ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcatcgc cgctatcgct
     2101 gatataccct tcatcacgca gtgtcagcac cagagaactg aacaccgcct tgtcgaagaa
     2161 ctccggcgcg ttgatgccgt gcagcacgga gagacgttgc gcgacggtgc ggctctcttt
     2221 ctccagcgta ccgcggttga tcgacgggtt ggcactcaac aaccagaagg tgatggcata
     2281 acgttgcagc gtttcgcgcg cgcctgcggc gagcagctgt agcgtgcgag aatgcaccgg
     2341 gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc
     2401 cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt
     2461 cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg
     2521 ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg
     2581 atagtaggtc atcagcaccg cttgctcgcg tggcagaatg atgatgtcgc cgatagtgtc
     2641 tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc
     2701 tgaaggaacg gtagagtccg tggagtaagg tacgttgcgc atcagatcca ggtagcagtt
     2761 gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt
     2821 acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc
     2881 agcaatatta ttgaccgtcg gcgttaacca ggccggacgc accgcttcga tgggatcgat
     2941 agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc
     3001 gaagttgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca gcatctgcgg
     3061 caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat
     3121 gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag
     3181 catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtgcg
     3241 ggaacgaccg ccttccacga agtactcgac ggaataacca cggctgaaca gttcgccgag
     3301 atactcacgg aaaacggtgg aataaagttt attgccttta aaagtacggc gaataaagaa
     3361 cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga tcccggcggc
     3421 gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagtagca ggtagtccat
     3481 gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca gctggcgaac
     3541 gcgctcggcg ttatggacgt tgatgccctg ataaagtcgg ttccaggtga agcccagaat
     3601 gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagcgc
     3661 aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac
     3721 cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcag
     3781 acgtgggccc acagcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag
     3841 tttctgagcg atagttttat ctgtgccgtg ttcatcggcc atgcggcgca gagaaactga
     3901 cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acggcgaaaa atttttgtac
     3961 gccgttaagc atacgcagcg gcgggtttac ttcgcctttt tcacgccccg gcgcgcgacc
     4021 aaacatcacc gatactggca ccatctgcac atccagattt gggttgctgc ggtgcaaatc
     4081 aagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg tgaacacacg
     4141 cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc
     4201 taacgggtca ggcaagtcat gagccagaca ctgggcgcgc aacgtcagca aatctgcttt
     4261 cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca gttccggggc
     4321 aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt
     4381 gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa
     4441 tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg
     4501 cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat
     4561 gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaatt atcaaagctg
     4621 ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg
     4681 aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcaatta
     4741 cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg
     4801 ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcaa
     4861 aagatatggg atccgccgcg gtgctgattg ccattatcgt cgccgtgatt acctggtgca
     4921 tcctgttatg gtcgcatttt ggataaccct tccagatttc gataaatctc tggtttattg
     4981 tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata actgtatata
     5041 cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
     5101 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
     5161 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt
     5221 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
     5281 ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca gcatattgaa
     5341 ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc
     5401 agcgggatgt cgatgaaaga tatcggcatt atggatggtg acttgctggc agtgcataaa
     5461 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt
     5521 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
     5581 ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggggtt
     5641 attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgcc aggcgtcgcg
     5701 gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat
     5761 aaagcactct ggcatctcgc cttacccatg attttctcca atatcaccgt tccgttgctg
     5821 ggactggtcg atacggcggt aattggtcat cttgatagcc cggtttattt gggcggcgtg
     5881 gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt acgcatgagc
     5941 accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg
     6001 ctggtgcaac cgttgctgtt ggcgttgggg gctggggcgt taattgcgct gctgcgtaca
     6061 ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcggtcct ggaacaggcg
     6121 cggcgctttc ttgaaatccg ctggttaagc gctccggcgt cgctggcgaa tctggtatta
     6181 ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc
     6241 aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctgcatat gaacgtgcag
     6301 ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg tctgctaatg
     6361 gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac tgcctggcga
     6421 ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc gctgttgttg
     6481 caactctgtt tcggcgcgat aaccgtactt ggtgcgcgac tggggagtga cattgtcgct
     6541 gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgcttga tggttttgcc
     6601 tacgcggttg aagcgcactc cggtcaggca tacggtgcgc gcgacggtag ccagttgctg
     6661 gatgtatggc gggcagcgtg ccgccagtcg gggattgtag cgttactgtt ttcggtggtt
     6721 tatttgctgg caggggaaca catcattgcg ttgctgacgt cgttaaccca gattcagcag
     6781 ctggctgacc gctatcttat ctggcaggtg attttgccgt tggttggtgt ctggtgttat
     6841 ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg
     6901 gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg taatcatggt
     6961 ttgtggctgg cattaaccgt ctttctggcg ttgcgcgggc tttctctggc ggctatctgg
     7021 cggcgtcact ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa aattctgaat
     7081 aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac
     7141 gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg
     7201 taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga
     7261 tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg
     7321 ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg
     7381 ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt
     7441 ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac
     7501 ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc
     7561 catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacact cttctttcac
     7621 cgcgccgcag cgatcgcaaa taaacatggc tgacgtatgg gtgggctggt cgaacagatg
     7681 acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa
     7741 atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagtaaatc
     7801 aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag
     7861 gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct gcgctaataa
     7921 ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac
     7981 tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg ttgattttct
     8041 gttttgtagg taaggtgttg tgttgccttg tcgtaccatt atcaacacga taataattaa
     8101 taattcattt ttaaataaat tgcttcaagt taactcgcta tttatttaac tgggtttaaa
     8161 cgcatacctg tagttcagaa attatgagcg atttttgata gtgaccagat ggcaaaagaa
     8221 ttataaggag gacacggtac gacaaaaaag gtagatgaat catgttgaag ataatacccg
     8281 gtgcaactgg atactttaat aaaacactca attcaaatca attcgataat gaagatgcaa
     8341 tcaaagataa attagataat agaggttcaa tcaaaggtaa attaaataat atatatggta
     8401 aatctattga ttatgcagcg ctgcgttatc gggatataat tatt
//
LOCUS       DAZKGS010000180         8418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKGS010000180 DAZKGS010000000
VERSION     DAZKGS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..577)
                     /gene="yeeO"
                     /locus_tag="ACHOQY_004162"
     CDS             complement(<1..577)
                     /gene="yeeO"
                     /locus_tag="ACHOQY_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9680147.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKI"
     gene            738..813
                     /locus_tag="ACHOQY_004163"
     tRNA            738..813
                     /locus_tag="ACHOQY_004163"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:771..773,aa:Asn,seq:gtt)
     gene            complement(851..1801)
                     /gene="cbl"
                     /locus_tag="ACHOQY_004164"
                     /pseudo
     CDS             complement(851..1801)
                     /gene="cbl"
                     /locus_tag="ACHOQY_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(1903..2820)
                     /gene="nac"
                     /locus_tag="ACHOQY_004165"
     CDS             complement(1903..2820)
                     /gene="nac"
                     /locus_tag="ACHOQY_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9680148.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3146..3221
                     /locus_tag="ACHOQY_004166"
     tRNA            3146..3221
                     /locus_tag="ACHOQY_004166"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3179..3181,aa:Asn,seq:gtt)
     gene            complement(3265..4212)
                     /gene="ldtA"
                     /locus_tag="ACHOQY_004167"
     CDS             complement(3265..4212)
                     /gene="ldtA"
                     /locus_tag="ACHOQY_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9680149.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(4274..5353)
                     /gene="cobT"
                     /locus_tag="ACHOQY_004168"
     CDS             complement(4274..5353)
                     /gene="cobT"
                     /locus_tag="ACHOQY_004168"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9680150.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5365..6108)
                     /gene="cobS"
                     /locus_tag="ACHOQY_004169"
     CDS             complement(5365..6108)
                     /gene="cobS"
                     /locus_tag="ACHOQY_004169"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9680151.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6105..6650)
                     /gene="cobU"
                     /locus_tag="ACHOQY_004170"
     CDS             complement(6105..6650)
                     /gene="cobU"
                     /locus_tag="ACHOQY_004170"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9680152.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6802..6975)
                     /locus_tag="ACHOQY_004171"
                     /pseudo
     CDS             complement(6802..6975)
                     /locus_tag="ACHOQY_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(6954..7142)
                     /locus_tag="ACHOQY_004172"
                     /pseudo
     CDS             complement(6954..7142)
                     /locus_tag="ACHOQY_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(8139..8418)
                     /locus_tag="ACHOQY_004173"
                     /pseudo
     CDS             complement(8139..8418)
                     /locus_tag="ACHOQY_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2271 a         2245 c         1808 g         2094 t
ORIGIN      
        1 gtattttcgt attccctgca ccacgaagtg ccccgctacc aatcaaagtg atggcaactg
       61 ctggataact gagtaccgtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
      121 tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa atgatgaata agcgttgcca
      181 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
      241 gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagcag
      301 caaaaaaagc cataatgacc atattgaagc tgtccgccaa tcccacgccg gccatcgcat
      361 cttttcccag ccagctgacc agaaaagtgc tcaggacacc catcaacagg acacaggcat
      421 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc
      481 tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
      541 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaataatgag ggagaatcag
      601 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
      661 acggcaaaca gttgggaaat ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga
      721 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
      781 atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc
      841 gggctttttg ctatatctga taatcaattt cctcttcact gttctccatc acctggcgct
      901 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac
      961 gttgaagttg ccctcgcttc agtcccaacc atacagtatt ggcatcgaaa agatgccgcg
     1021 tatccagacg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     1081 caatccccag cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata
     1141 caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag caccccgtaa
     1201 tcccctatcg gtaagtgatt aacggccact tcgctattga ttccagcgtc aacggtgtaa
     1261 tttgcgtcaa gggatgatcg agtggaacaa gcaaactatg gtgccaacga aaccacggga
     1321 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     1381 cattttgcaa caacgtcgca atttcctgtg gcgtaccctg gattagctcc agccgaacct
     1441 ccgggaaaag ttcgcgaaaa gctttaatga cctcaggcaa gctataacgt gcctgagtat
     1501 gcgtcgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac
     1561 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     1621 cagtcatacc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatccc
     1681 ccagttcccg aatatgacgg ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct
     1741 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     1801 cggtaaactc cgggcagctc agatttcccg ttattgttaa agtctaatgc ccggcataac
     1861 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaattgcca
     1921 ctgccttttt tccatcactg gagaactaat cactgacatt aacaactctt tcactgcctg
     1981 tgcctgtggc gataagttcg ctctggcggg taaatttaat gacaaagaga gactcatgga
     2041 aggcgtggta atgcgtgaca tccacccatt tactgcgcca cataacgaac gcgcggccga
     2101 ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga
     2161 ctcaatttca ccaatgactt ttgccgtgag ccgccgtagg gaaaaagcct catcaacacg
     2221 aagtctaaca gcactgtaat cactggggag aaagaggttc atttgcgcaa tagcattaac
     2281 atcaacgctt tgccccgggc aatcttgagt tcctaccagg aaaagatctt ctttcagcaa
     2341 agcctgactg gataccccag ccacagggga atgctcataa atcaccgcca tatcgagttg
     2401 gtgatttatc agtttttcgt taagcactgc accgctattt tcatgaagat agataacgat
     2461 ctccgggaat tcagcgcgaa ccgcttgtaa taagggcatg gtgatagatg acgcagcggt
     2521 tcctggcgca aagccaatcg agacttgccc cgataatgcc tgaccaacgt tatgcaccgc
     2581 cagttgggcc tgctcacact gacgtaaaat ggcccgcgca tgggtataga gaatttttcc
     2641 ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa agttgttgat ttaactcacc
     2701 ttccagtgtg gcaacctgct ggctgagcgc tggttgtgcg atatgcaata cttcagcagc
     2761 ctgagtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat
     2821 gttgcctccg gtttttgaga atcggcccaa gtgccgccat tacttacaac cagactgcaa
     2881 gatgcttgcc agttttattt tggcgttgat gtacaagcta accaactgtc aaataagaga
     2941 ttatgataga ttcgtcattg gctcttttca ccagcagtcg cgttcccctg ccctataaaa
     3001 ggagggtatg caccacgatg gttcattacc aataagattg aaatctcatc actttgttga
     3061 aattggcagc aaacaaacaa aaaaatgcat ttcacccttt gacatcacca tgcactgcca
     3121 ttaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt
     3181 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcaaaa aagcctgctt
     3241 tctagcagga tttttgcttt ctaattacca acgctcataa aacatctgtc ttgaaccaga
     3301 actaatttgc acaggcattc ccgatcgacg ttgcaacgca gcatttgcgc gattcacatc
     3361 aacttcttgc ccgttgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg
     3421 gtcagactca tattctgcac gattgcgcga cagcggctca tgaacttcca gccagttcga
     3481 gccatctggt tcagtggtgt attttactgg ctggtcgata atttgcacac gcgttccaac
     3541 aggaacatta tcaaacagat atttgatatc gtcattgcgc agacgaatac agccctgact
     3601 tacccgaagt ccaataccaa aattggcatt ggtaccgtgg atggcataca acctgccaat
     3661 ataaatcgcg tacagcccca tgggattatc gggccccgca ggaacaaatg cgggcaaact
     3721 ctcccctcgt ttcgcatatt cgcgccgagt gttcggcgtt ggcgtccagg ttggagcttc
     3781 ttgtttacgt tcaacggtag tcacccagtt acgcggggtt tctcgcccag cctggccgat
     3841 accaatagga aagacttcca cagtattact gtctggcggg tagtaataaa gacgcatctc
     3901 agcgacgtta accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat
     3961 ggtgagttgc gagccagact tcggcaagaa aacatcagcg cccggattcg cttccagcat
     4021 gttacttaac ccttgcccgt attgtgcggc aaaagtctcc agcggctggg tattgtgatc
     4081 aggaacagtt acagtaagcg actgccccac caaacggcta ccctctggag gtaatggata
     4141 agttaccgcc aggctagtat ggctggcaaa aagcagagca aatgagcaaa gaatatttac
     4201 acgacgcatc atgtcccttt cctatgtcgc gaaagctatc cgttaagtat agcttttatc
     4261 agacttttcg tttttaactg ttcaaatcag aagtcgtatt ccccggtaga acaatattac
     4321 tggcagcaag ttcgcccatg ttgttatata tcgcacaggc agcttcgatg atgggcatcg
     4381 ccagagctgc gccactcccc tcacctaaac gcatctccat attgagataa ggctccagcc
     4441 ccaaatgcga cagcgctata cgcgcccctt tttctgccga caggtgagaa ggaatgagat
     4501 acggtttgat tgcaggagac atctggcagg ctgcgagcgc agcagcataa gaaagaaatc
     4561 catccagcaa cacgggtaaa ccgcaggaag cagcgcctaa catcacgcca gccattccga
     4621 ccaaatcaaa tccccccact tttgccagaa catcaacgcc atcctgcgga tttggttgat
     4681 tcaacgtaat cgcccggcgc acaacatcaa ttttattagc cagtttatct gtcggcaggt
     4741 ttgcgccaat cccaaccact tcttcaggat cccggccagt gattgtgctg actattgccg
     4801 ctgccggtgt cgtgtttgcc atccccagtt cacctacacc aaacagcgtg acaccgtttt
     4861 ttgccagctc ccgcgtataa catatgacgt ccaaaagcaa cttttcagcc tgacggcgac
     4921 tcattgccgg agctgaagca atattgccgc tacctcgtgc gacacgcatg ttgataagcc
     4981 cggggatagg ctcagcacta tcaataccaa catcaactac gtggacgtta gcgcccgctt
     5041 gtgctgccag cacacacacg ccggttgttc cgcgggtcat attttcagcc tgaatggctg
     5101 tcacttcttt tggggaaata gcgacccctt cctcccagac gccgtgatcg gcacacataa
     5161 ccagtaccgc ttttttgccc acatgcggta tgccattcaa ccccggcatt cccgccagtt
     5221 gtatggcaag cgcctccagc cttcccaggc taccaacagg tttgagtaac ccgtcaatat
     5281 gccgttgtgc acgcgacata gcggcaggat cgatagcagg gatcgtattc agtaaatcgg
     5341 caagtgtttg catgtcacgt ctctttatag cagtgccagc aggaagacca gttcaccaag
     5401 ctctatagca gctcccagcg tatcgccggt ttgcccgccc agcgtacgtt tgagaagctg
     5461 accgaggatg aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccgg
     5521 caataatacg gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc
     5581 aattttgcca atgaacacat tgccaagccc ttcttcccgg gcataacgat gacgatacat
     5641 caataatgcg gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcgg
     5701 ttcgccacgc agagccaact cgcttaatac cagaatcttt gccagtacaa caaaaatcaa
     5761 tgccagaccg ccatgggtgc ctaaacggct atcacgcatg atttccaaca tgcgatcgcg
     5821 gctacgtgca gaaaatacgc catcacaggt atcggcgagg ccgtctaggt ggaatccccc
     5881 ggtcatcagc gccagcacaa gtacgctaaa cagtgccgcc agtggtacgc cacaccatgc
     5941 ctgcagcacc atgaagacca gcccgctaat cgcgccaagc aataatccaa ttaaaggaaa
     6001 agtaataata ccgcgagaat aatgctcgaa atccagtccc tgggacctgc gacgcggtac
     6061 aggcagacgc gtaataaatg agagcatcgc ccaaaataat ttactcattt gatttttact
     6121 ccaatacccg aaaccaccag ccatacttca tttgccgcag cggccaactg ctgatttacc
     6181 cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc
     6241 acttcgttag tcactaatac aacctttgcg gggcaacgtt ggcaggcagc aatcaacgac
     6301 tgaatctcag cattaatcgc ctgttccatc gcctgataat cccattcatc agggtcttta
     6361 tcgccgccat aatcaaacaa cagattagtc accattgtgg taacgcattc aagcaacaca
     6421 acctcattcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact
     6481 gtgcgccagt gctccgggcg gccttgccga tgatgttcta tccgtgcagc catctcatca
     6541 tcaaggattt gcgaggtagc gatatacaga acctgtgaag agtccccaat aagcgcctct
     6601 gcgtggcgac tcttcccgct ccgtgcgccc cccgtcacca aaatcatcat accgactcct
     6661 gattcaaatg tatgatggta ttcattgaac agaacaatgg atcccccacc gttgctgacc
     6721 gcaaaacatt tacgcgatta atactgcgcg taatataatt aaaaatccag cattctcaat
     6781 ccattacgaa ataagatggc attacggaat agccagactt tctcgtgtta acgtccgaca
     6841 aaatgcgtca ttgacagcgg tttctgtgga ttgacacatt tcattcagat aatgaattaa
     6901 tgctactgcc ggaacaacca gcaaacgggc attgtgttct gaaatccagg ctattgattc
     6961 aacgtcagcg acagatctgc gctgtaaaag aacttgtaaa acaacgtaat agaattatcc
     7021 ggaatggtgg cgactatgca ctagaacacc acaatttcgc tctctcggta aggcactgtc
     7081 acagcattca acagaatgtg acttgccaga tttattagcg ccaccagatg tttaaccggg
     7141 taaccacgac ccagtcgggg atatattcca tcttctcgct gacatcatca agaataaaaa
     7201 ggtttatcac actcagagca acgttcttca gcaggcggga taattcttca acatttagaa
     7261 gatgtgttat taaaggcata caaactggac gtattgtatt ttcttttggt ggttgaccta
     7321 aggtagcagt ttatcctgat gcgctgagat ttctgtaata tctacgtcaa cattctcctc
     7381 gaatagaaaa tgcagccccg gcaagtgatt cacatttttt aacaaaacgt tgttagcgtg
     7441 ccattttcag aagctctcca agaaagcgaa ttcttttctc aaattctgca ttctcagaca
     7501 cagctttcta ctccatggta atggcccaaa tacgaagttg ctcaggatca ttagtggtgc
     7561 tgagaagtta aataaccatg cggagtacca ctcgtcatca cgtatttata tatcatgaag
     7621 ctattgttgt gttttgtaca tcagtagata tattctgata tactcctttt gctagacata
     7681 acctttcacc tgcttgcaaa gcttctgtgt tctgacattg ccaaattgtt gcaattctgt
     7741 atccagcctt ctttcagtca tagcttcggg ccgcgataag actcactgat ctgaccctga
     7801 ttcctcttgc agactttata gaccaattaa aatgcagttt ctgcaggtca acgtctgacc
     7861 atcattgtca tcactctggc cattagagta accttctgca ttcatccttt tgtaaaaagt
     7921 ttatgtcagt atcagcaatt aaccggacct gatactgata tgagtcttac cgcatatacg
     7981 gtcaatttca gcaattaatt acattatcca cgccaaagtg tttgtcatcg caatgatggt
     8041 accttctttc agacaccatt ttttcaactc cgttttccac ggaccgcact cttatgtcaa
     8101 gagtgcggtg atcctaccca cgtaatatgg acacaggcct aagcgaggtt ctggttttca
     8161 aattgttccg gactgaggcc gccacaccaa ctgtgccgcc gccaccgatt gtaatcacat
     8221 tcgatataat taaacaccgt tgcccgcatt atttcccggc tgataaagtg ttctccatgg
     8281 atacattcca ctttcagcga atgaaagaag ctttccacgc agattatgcc gcttcagttg
     8341 cgcctgataa tctgctgaac agtactggcc tccacggtcc gtgtgaacga taacgttccg
     8401 gggcctctta cgccgcca
//
LOCUS       DAZKGS010000181         8416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKGS010000181 DAZKGS010000000
VERSION     DAZKGS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..364)
                     /locus_tag="ACHOQY_004174"
     CDS             complement(158..364)
                     /locus_tag="ACHOQY_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9680153.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            677..766
                     /gene="kdpF"
                     /locus_tag="ACHOQY_004175"
     CDS             677..766
                     /gene="kdpF"
                     /locus_tag="ACHOQY_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9680154.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            766..2439
                     /gene="kdpA"
                     /locus_tag="ACHOQY_004176"
     CDS             766..2439
                     /gene="kdpA"
                     /locus_tag="ACHOQY_004176"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9680155.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2462..4510
                     /gene="kdpB"
                     /locus_tag="ACHOQY_004177"
     CDS             2462..4510
                     /gene="kdpB"
                     /locus_tag="ACHOQY_004177"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9680156.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4519..5091
                     /gene="kdpC"
                     /locus_tag="ACHOQY_004178"
     CDS             4519..5091
                     /gene="kdpC"
                     /locus_tag="ACHOQY_004178"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9680157.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5084..7768
                     /gene="kdpD"
                     /locus_tag="ACHOQY_004179"
     CDS             5084..7768
                     /gene="kdpD"
                     /locus_tag="ACHOQY_004179"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9680158.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7765..>8416
                     /gene="kdpE"
                     /locus_tag="ACHOQY_004180"
     CDS             7765..>8416
                     /gene="kdpE"
                     /locus_tag="ACHOQY_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9680159.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPNPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITET"
BASE COUNT         1763 a         2179 c         2447 g         2027 t
ORIGIN      
        1 gtcagataag aacaattagg aaaatattta tatatatttc atgaatatat gtttcgattg
       61 gtataacgat gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt
      121 tccgctggcg gttttcaatg tattcaaaac tcagcaatca ataaaaatca ccagttgcct
      181 tttcggcctg atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt
      241 agctgtaaaa gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg
      301 ctgcaaccgc ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt
      361 ccatactttt ctcccccttt tgtatctacc cggtgaatgg taccggaaaa atgaatttgt
      421 ttatctgatg aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt
      481 tttgcttatt gatggtttat ttacattcat cctgtaatta agttacacaa aagttaaatt
      541 aatactaaac attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc
      601 ccgcagattt ttgcgaaatc tttgcagcca gaattctacc cttccggtat cacttttagg
      661 ccactggagg tgcactgtga gtgcaggcgt gataaccggc gtattgctgg tgtttttatt
      721 actgggttat ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg
      781 ttcttactga tcgccacgtt tttactggtg ttaatggtgc tggcgcgtcc tttaggcagc
      841 gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt tgaacgtata
      901 ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata tctttgtgcc
      961 attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cgatcagcac
     1021 tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatgcc
     1081 gccgtcagct ttgtcacgaa taccaactgg caatcttaca gcggtgaaac cacgttgagc
     1141 tatttcagcc agatggctgg cttaacggtg caaaactttc tttctgccgc cagcgggatt
     1201 gcggtgattt ttgccctcat ccgtgcgttt acccgccaga gcatgagcac actcgggaat
     1261 gcctgggtcg atctgctacg catcacgtta tgggtgctcg tccctgtggc gttgttgatc
     1321 gcactgtttt ttattcaaca aggtgcgctg caaaactttc agccttatca ggctgtgaat
     1381 accgttgaag gagcgcaaca gctgttaccc atggggcctg tagcttctca ggaagcgatc
     1441 aagatgctcg gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa
     1501 aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacggcg
     1561 ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg
     1621 gcgatgtcag tgatttttgt catctgcgta ggcgtggtga tgtgggcaga agttcagggt
     1681 aatcctcatc tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc
     1741 cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc ttcctgtggc
     1801 gcggtgattg cgatgcatga ttcgtttacc gctctcggtg gcatggtgcc gatgtggctg
     1861 atgcaaattg gtgaagtggt gttcggcagt gtcggttctg gtctttacgg catgatgctg
     1921 tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg
     1981 ggtaaaaaaa tcgacgtacg cgagatgaaa ctgactgcac tggcgattct ggttaccccg
     2041 acgctggtgc tgatgggcgc ggcgctggca atgatgaccg acgccggacg tagcgccatg
     2101 ctcaaccctg gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaac
     2161 aacaacggca gcgcctttgc cggattaagc gccaactccc cgttctggaa ctgtttactg
     2221 gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt
     2281 tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg
     2341 ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct
     2401 gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt gagcactgaa
     2461 tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga
     2521 agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg
     2581 gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcctgg
     2641 caatgcgctg tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa
     2701 tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagtc ttaaaggggt
     2761 gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa
     2821 agttcctgcc gatcaactgc gtaaaggtga catcgtgctg gtagaagctg gcgatattat
     2881 cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg
     2941 ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac
     3001 gcgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggtg agacatttct
     3061 ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga acgagattgc
     3121 cctgactatc ctgctgattg ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg
     3181 gccgttttcc gcgaggggcg gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt
     3241 ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag
     3301 tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga
     3361 cgttgacgtt ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc
     3421 ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc
     3481 ttcgctggct gatgaaacgc cggaaggccg cagtattgtg atcctcgcca agcaacgttt
     3541 taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca
     3601 aagccggatg agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc
     3661 cattcgccgc catgttgagg ctaacggcgg tcacttccct accgatgttg atcaaaaagt
     3721 cgatcaggtt gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt cccgtgtgct
     3781 gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct
     3841 gcgcaaaatg ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc
     3901 cattgctgcg gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct
     3961 ggcattgatt cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac
     4021 caacgatgct ccagcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggtactca
     4081 ggcggcgaaa gaagcgggca atatggtcga tctcgactct aacccgacca agttgatcga
     4141 ggtggtacat attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat
     4201 tgccaacgat gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacgtatcc
     4261 gcagttaaat gcgctgaaca tcatgcgcct tcattcgccc gactccgcca ttctcagtgc
     4321 ggtcattttc aacgccttga ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag
     4381 ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg
     4441 tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg
     4501 tctggtgtga ggtttaccat gcgtggctta cgtccggcat tatcgacatt tatctttctg
     4561 ttattgatta ctggcggcgt ttacccgctg ctgaccaccg tgctggggca atggtggttt
     4621 ccctggcagg ccaacggttc gttgattcgt gaaggtgata cggtgcgggg ttcggcatta
     4681 atcgggcaga attttaccgg caacggctat tttcagggtc gcccgtcggc aacggcagaa
     4741 atgccctata atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg
     4801 gataaacaaa tagccgcacg cgttgctgca ttacgggccg ctaacccgga tgccagtacg
     4861 aacgttccgg ttgaactggt gacggcatcg gcaagcgggc tggacaataa tatcaccccg
     4921 caagcggcgg tctggcaaat cccacgcgtg gcgaaagcgc gtaatctcag cgttgaacag
     4981 ctcacgcaac tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg
     5041 gtcgtcaaca ttgttgaact caatctggcg ctggataaac ttgatgaata acgaaccctt
     5101 acgtcccgac cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa
     5161 agttttcttc ggtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca
     5221 gcgactgcgg gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc atgggcggaa
     5281 agataccgcc gccatgcttg aagggctggc tgttctgccg cccaaacgcc aggcgtaccg
     5341 tgggcggcat atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcgctgat
     5401 cttaatggac gaactggcgc acagtaatgc gccaggttct cgtcatccta aacgctggca
     5461 ggatatcgaa gaactgctgg aagctggcat tgatgttttc acaaccgtca acgttcagca
     5521 tctggaaagt ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt
     5581 gcccgatcct tttttcgatg ccgccgacga cgtagtgctg gtggacttgc ccccggacga
     5641 tctgcgccag cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat
     5701 tgaacatttt ttccgcaaag gcaatctgat cgccctgcgt gaactggcac tgcgccgtac
     5761 tgccgatcgc gtagatgagc aaatgcgcgc ctggcggggg catcctggtg aagagaaagt
     5821 gtggcacacg cgcgacgcga tccttttatg catcgggcat aacaccggca gtgagaaact
     5881 ggtccgcgcg gcggcgcggt tggcctcgcg cctgggtagc gtctggcacg cggtgtatgt
     5941 tgaaacccct gccctgcatc gcttaccgga aaaaaaacgt cgggcaattc tcagcgcctt
     6001 acgtctggcg caggagctgg gcgcggagac ggctacactt actgatccag cggaagaaaa
     6061 agcagtagtg cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccggc
     6121 ctcgtgccgc tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga
     6181 tctcgatcag gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc
     6241 ggatagccgc tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc
     6301 cgcgttatgc gccgttatca ctttaattgc catgcagtgg ctgatggcgt ttgatgccgc
     6361 caacctggtg atgctgtatc tgcttggcgt ggtggtggtg gcgctatttt atggacgctg
     6421 gccttcggtg gttgctaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc
     6481 acgcggcacg ctcgctgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac
     6541 cgtcgggctg gtgatcggga accttactgc gggtgtgcgt tatcaggcgc gggtagcccg
     6601 ttaccgcgag caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg
     6661 cagtccgcag gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg
     6721 cagtcaggtg ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg
     6781 aatgacgcca tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg
     6841 tgcgggcacc gacacgttac ccggcgtgcc gtaccagatt ttgccgctaa aaagcggcga
     6901 gaaaacctac ggactggtgg tggtggaacc ggggaatctg cgtcagttga tgatcccgga
     6961 gcagcagcgc ctgctagaga cgtttacgct gttagtcgcc aatgcgtttg agcggctgac
     7021 gctgaccgcc agtgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc
     7081 cctgctggcg gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc
     7141 agaaatctta acgctcgatc tggcaagcga aggatcgccc cacgcccgcc aggccagcga
     7201 gattcgtcag catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg
     7261 aattcagtcc ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg
     7321 cagcgctctg caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga
     7381 accgctgacc ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct
     7441 ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga
     7501 gggcgaaaat ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga
     7561 gcagacgata tttgataagt ttgctcgcgg gaataaagag tcggcagtac cgggggtagg
     7621 gcttggactg gcaatttgtc gggcgatagt ggatgtacac gggggcacta ttaccgcgtt
     7681 caaccgaccg gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga
     7741 acttgaagac tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct
     7801 attcgtcgct ttctgcgcac ggcgctggag ggcgacggga tgcgcgtcta tgaggccgaa
     7861 acgctgcaac gcggcttgct ggaagcggct acccgtaagc cagatttgat tattctcgat
     7921 ctcggcctgc ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg
     7981 gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg acaaaatcgc cgcgctggat
     8041 gccggagcgg atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg
     8101 cgcgtcgctt tacgccgcca ctccgccacc gccgcgccca atccgctggt gaaattttcc
     8161 gatgttaccg tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc
     8221 acgccgattg agttccgcct gctggcggtg ctgctaaata atgccgggaa agtgctcacc
     8281 cagcgccagt tactcaatca ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg
     8341 cgtatttata tgggacatct gcgacaaaaa ctggaacagg atcccgctcg cccccgccat
     8401 ttcattactg aaaccg
//
LOCUS       DAZKGS010000182         8398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKGS010000182 DAZKGS010000000
VERSION     DAZKGS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(177..253)
                     /locus_tag="ACHOQY_004181"
     tRNA            complement(177..253)
                     /locus_tag="ACHOQY_004181"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Asp,seq:gtc)
     gene            complement(386..1117)
                     /gene="dnaQ"
                     /locus_tag="ACHOQY_004182"
     CDS             complement(386..1117)
                     /gene="dnaQ"
                     /locus_tag="ACHOQY_004182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9680160.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1182..1649
                     /gene="rnhA"
                     /locus_tag="ACHOQY_004183"
     CDS             1182..1649
                     /gene="rnhA"
                     /locus_tag="ACHOQY_004183"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9680161.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1646..2368)
                     /locus_tag="ACHOQY_004184"
     CDS             complement(1646..2368)
                     /locus_tag="ACHOQY_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9680162.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2402..3157
                     /gene="gloB"
                     /locus_tag="ACHOQY_004185"
     CDS             2402..3157
                     /gene="gloB"
                     /locus_tag="ACHOQY_004185"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9680163.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3229..4587
                     /gene="mltD"
                     /locus_tag="ACHOQY_004186"
     CDS             3229..4587
                     /gene="mltD"
                     /locus_tag="ACHOQY_004186"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9680164.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4635..5405)
                     /locus_tag="ACHOQY_004187"
     CDS             complement(4635..5405)
                     /locus_tag="ACHOQY_004187"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9680165.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5483..6283)
                     /locus_tag="ACHOQY_004188"
     CDS             complement(5483..6283)
                     /locus_tag="ACHOQY_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9680166.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6524..7438
                     /gene="yafC"
                     /locus_tag="ACHOQY_004189"
     CDS             6524..7438
                     /gene="yafC"
                     /locus_tag="ACHOQY_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9680167.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7435..8238)
                     /gene="dkgB"
                     /locus_tag="ACHOQY_004190"
     CDS             complement(7435..8238)
                     /gene="dkgB"
                     /locus_tag="ACHOQY_004190"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9680168.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2258 a         1994 c         2029 g         2117 t
ORIGIN      
        1 taaattgaat gattcgatag tatgattaaa tattaatgat atggtctacc atatgtgatt
       61 aacacattta cagcataaca aaaccataga ccgaagaaat agcgggattt aaatgcaaaa
      121 aaaaccgcaa aattaaaaat cttgcggttc tctgaactca ttttcatgag tgaatttggc
      181 ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact
      241 gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg attgcctcac
      301 ccttcgtcaa cggtttttct catcttttga atcgtttgct gcaaaaatcg cccaagccgc
      361 tatttttagc gccttttaca ggtatttatg cccgccagag gcagcttccg cccttcttct
      421 ccaccagatc aagacgggct tcatgagctg caagctcttc atctgtcgca aaaacaacgc
      481 gtaacttact tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt tgctgctgtg
      541 tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc agataaactt
      601 ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt tcgtttactg ttatctattt
      661 cgtagcgggc acataacgca tcgaggctgt tgcgcttacc aggaaacatt ttcctcgcca
      721 ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg cgcttaagca
      781 acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc accaactccg
      841 cgccgcggat atagtccatg aactcatcgg ctacttcggc aaacgtgggc ttatcgagca
      901 aaaattcatc ggcaatacca tgtacgccaa aggcttccgg atccaccagc cgatcgggtt
      961 tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca acggcaccaa
     1021 tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg gtggtttcgg
     1081 tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat gtcagacttg
     1141 tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa caggtagaaa
     1201 ttttcaccga tggttcgtgt ctgggtaatc ctggacctgg gggttacggc gctattttac
     1261 gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc aacaaccgta
     1321 tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc gaagtcattt
     1381 tgagtaccga cagccagtat gtccgccaag gtatcaccca gtggatccat aactggaaaa
     1441 aacgtggctg gaaaacagca gacaaaaaac cagtaaaaaa tgtcgatctc tggcaacgtc
     1501 ttgatgctgc attggggcag catcaaatca aatgggaatg ggttaaaggc catgccggac
     1561 acccggaaaa cgaacgctgt gatgaactgg cccgtgctgc ggcaatgaat cccacactgg
     1621 aagatacagg ctatcaagtc gaagtttaag cctgtggttt acgatattgc cgggtggctc
     1681 caaccgcctg gcgaattcgt ggcttgtttt tactctgttt catcggattt agcgttaaag
     1741 gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga atatgtgcat
     1801 tcaatagttt tccaccgtgt ttgttccacg ggagaacgtg gaaacggctg gcgtgcagca
     1861 cttcaaaatt caacaaagag agccagtcca gttgccgcat cagagtaaac atccggctgt
     1921 tatagggcga ggtttttcgc aataccggca caagtttgcg taatcccatt aaactaatgg
     1981 gattgaagcc actaatcacc agccagccat catcaatcaa tacccgatcg gtttcacgca
     2041 ataaacgatg cggatcggtg caccacggca atgtatgtgc cagtagacaa acatcaacgg
     2101 atttatcggc aaaaggaaga tgaagtggat ccgcctgcac ctggacgggc attccttgcg
     2161 cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct gcgcttaaat
     2221 tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg agctggcgct
     2281 ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcgatca ggagccacga
     2341 cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacacta agaggtaatc
     2401 tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt tgaatgatga
     2461 agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccagtattaa acgccattgc
     2521 cgccaataac tggcaaccgg aggccatatt tctcacccac catcatcacg atcacgttgg
     2581 cggcgtaaaa gaactggtag aaaagtttcc gcaaattgtg gtgtatggtc cacaagagac
     2641 acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg ttttggggca
     2701 tgaatttagt gtaattgcca caccgggtca cactttagga catatctgtt acttcagtaa
     2761 accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtaggt tgtttgaagg
     2821 gacagcatca caaatgtatc aatcacttaa taagttaagt gcgttacctg acgatacatt
     2881 ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga gtattcttcc
     2941 gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg caaaaaatca
     3001 aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt taagaacgga
     3061 agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac ctgaagagcg
     3121 ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg tcatcggctc
     3181 cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat gaaggcaaaa
     3241 gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa cgttcaacag
     3301 cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac aagtcaggca
     3361 cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc tttcattggc
     3421 gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa acagaaatat
     3481 ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta tatgtactgg
     3541 atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact acccatagtg
     3601 gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat ctggcagatc
     3661 attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga cgcgcgtcgc
     3721 gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa caagatgttt
     3781 gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg agtcatgaag
     3841 gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt accgttgccg
     3901 caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct caaaaacagc
     3961 aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc gcgtgtgcac
     4021 ctgagcagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc cgtcagcaag
     4081 ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg tccgcagtac
     4141 gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctggcttc aggcgaaatt
     4201 gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg tgtttacacc
     4261 gtacgctctg gcgacacgct ttcaagtatc gcttcacgtc tcggcgtaag caccaaagat
     4321 ttgcagcagt ggaacaaact gcgcggatct aaactgaagc caggccaaag tttgacgatt
     4381 ggcgcaggca gtagcgcaca gcgactggca aacaacagcg atagcattac gtatcgtgtg
     4441 cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat caaagatgtg
     4501 atgcgctgga acagcgacac tgcgaatctg caaccaggcg ataagctgac gttgtttgtg
     4561 aaaaacaaca acatgccaga ttcctgacaa accagataat aaaaaggcac cgattccccc
     4621 ggtgcctttt ttatttatgc cgctttatgt gcttctacca tgatgatatc actggtgaaa
     4681 gagccgtcat tctgcaaggc aaaatacgtt ctcacctctg tcgatgcgct ctgttggtaa
     4741 atgcgaatag cgtctattaa cgcttctggc gtacgcattc tcacgaccca tgaagaaaat
     4801 tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc attgattaac
     4861 gtcaaccact caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa tgcttctacc
     4921 gtctgtaacc agatgtcgcg cactgggtga cccggagaca ttacgtccat cacaatcagc
     4981 ctaccgccag gtttcaatat ccggttcact tcacgcagtg ctgcaccaac atcatgccag
     5041 tgatgggcag aataacggct gataacaata tcaaatgcgt tgtcggcaaa tggcagactt
     5101 tcggcataac cctggcgggt ggtgatattt ttcagttgcc ggacttcggc agcttgtgcc
     5161 acgacatcta gcatgtgggc agataagtca tacgccacca ccgtgctcac gttttgcgca
     5221 gcgacaaagc tggcatgccc tgctccgcag cccatatcaa gtacacttgc accaggataa
     5281 tcagccagac gcaccgccag gcgctgtaaa tctcgtccgg atgcatgtac ggtactggtc
     5341 agatattcgc tggcttgaga gctaaactgc ttttctacat ggtcatggtg ggattgtatt
     5401 gtcattgcgc tgtccttttg ttatttgagt ataaaagggc agcgccaaac gttggtctgc
     5461 cctgtggcag acctgacata ccttatttat caggcttgcc gggactgaat tcaacgagta
     5521 gcggattgtg atcggaagcg cgcgtaacca gtacagaagc ttcgctgacg ttcagaccac
     5581 ggtagaaaac aaaatcgagc gggcgaccaa acgcccgacg gcgctgatca tcggtaaaac
     5641 gcacctggcg cagcgacatt tcccgcgcaa agcgatataa cgcgttcatc cttctacggc
     5701 tccaggcatt gaaatctccc gccataatga ccgggccgct gtggtgagct atctgatcac
     5761 caataggaag taactgctta ctatagacat ccacgcccag actgaagttg acggcgtgta
     5821 tattaaccac catcaacagg cgggtgtcag gtaatggata gaccgtcacc agtgccgact
     5881 tcgccagacg caaaatgggt tctcgttcac gtaacgggca gcaatacact ggatgcgccg
     5941 ccgaaagggt cattacaccg gaaggatgtt gtggcagcac gaaagcgggc acctgatcgg
     6001 cggcaagata gttagcggtc gcaaactgta ctaactctgg cgttgtctgc gcttcctgca
     6061 ataacaccag atgtgcatct ttgccgtagt tctttaatac cgacaaccat tcagcgcgtt
     6121 gctgtttgta tatgttccac accaggatcc gaatacgctc ttcagtactt aacggttctc
     6181 caggtggtaa tgcctggccg atgctcgcaa aagaccccgg cggtaagatc ctttccgcag
     6241 gttgtccggc aacatagcgc atggcatagg tgttttttcg cacttttgac tttgaaacct
     6301 ctattacgtc aacccttccg cactcgggag ggttctactg ttatagggat tttagctcac
     6361 tctttcaacg agcaattgta tattgttatg taagcaagtg cttacacagg tcagaatttt
     6421 gctcattatt gagaacatta aatcggatga catatcacgc atcctgatgg tttaatgaca
     6481 aataaattca aaatatctct tctattttgc aacaggagca aaaatgaaag ccacgtcgga
     6541 agaactcgcc atttttgttt cggtcgtaga aagcggcagc tttagccggg cagcggaaca
     6601 attagggcaa gcaaactcag cggtaagccg ggcagtgaaa aagctggaga tgaaacttgg
     6661 cgttagcctg cttaatcgga ccacgcgaca acttagcctg acggaagaag gccagcgtta
     6721 ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg gcagaatcag aaattatgga
     6781 gacgcgtaat acaccgcgtg gactgttacg catcgatgcc gcaactccag tggtgctgca
     6841 ctttctgatg ccgttaatta agcctttccg tgaacgctat ccggaagtta ctttatcgct
     6901 agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa gtggatgtcg cgatacgcgc
     6961 tggtacgtta acggattcca gcttacgtgc caggccgtta tttaacagtt atcgaaaaat
     7021 tatcgcctcc cccgattata tttcccgcta cgggaagcca gaaacgatcg acgatttaaa
     7081 gcaacatgtt tgcctgggat tcactgaacc cgcttccctc aatacctggc cgatagcctg
     7141 tagtgatgga caattacatg aggtgaagta cggtttgtca tccaatagtg gggaaacact
     7201 gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg tccgactaca tgatcgacaa
     7261 agaaatcgtt cgcggagaat tggtggagtt aatggcagat aaagtgttgc cagtggaaat
     7321 gccattcagt gcagtctatt acagcgaccg tgcggtaagt acgcgcatcc gggcttttat
     7381 cgatttcctt agcgagcatg taaaaacagc tcccggagga gctgtcagag aggcttaatc
     7441 ccattcagga gccagacctt ccgggctaac caggcggtcg ttgcaatcca gtgcggcgat
     7501 cgctttttta tcttcggcat caagctgtaa attttgtgcc ttaagattac tttccaggtt
     7561 ttcacgttta gtagaagaag gaattactga gtaaccttcc cccatagccc acgccagaat
     7621 cacttgtgcc ggagtcgcat tgtgtttagc tgcgatacga gcaataacct catctttcaa
     7681 ggctttccca taagccagcg tcatatagga agtaatatgg atgccgtgct gtttagccca
     7741 ggcaaccact ttacggtttt gcagataagg agagagttca atctggttag tagcgatgtt
     7801 ttcagcgcca ataacagcaa tcgccttttc catcaatggg atcgtgaagt tggaaatacc
     7861 gatctcacgc gtcagccctt gttttttggc ttccagcagc gcctgcataa actcttcaac
     7921 agagacttca tcgtttggtg acggccagtg gattagcgtc agatcaacat aatcggtacg
     7981 caatttttgc aggctctctt tcagacttgg gatcaatttg tctttgctga gattttcaat
     8041 ccagatttta gtggtgatgt agagttcatg acgtggcacg ccactttctg caatcgcctg
     8101 acctactgcg gcttcgttat catagatttg tgcggtatca attgcgcgat aaccaagttc
     8161 aagcgccgtt ttcacagatg aaataacaac gtcgtctttc agacggaaag tacctaaacc
     8221 aaatgcaggg atagccataa tatgcctctt ttaatgctat tttcgttaac tgaggtgatt
     8281 atgggcttca gactgttgag gaaaaagagc gtaaaatgca gaggattttt gcgattctgg
     8341 caataataga tatccagacg aaaaaaaacc tcgagcgtta actcgaggct tcttaata
//
LOCUS       DAZKGS010000183         8377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKGS010000183 DAZKGS010000000
VERSION     DAZKGS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..1636
                     /gene="dcuB"
                     /locus_tag="ACHOQY_004191"
     CDS             296..1636
                     /gene="dcuB"
                     /locus_tag="ACHOQY_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9680169.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1714..3360
                     /gene="fumB"
                     /locus_tag="ACHOQY_004192"
     CDS             1714..3360
                     /gene="fumB"
                     /locus_tag="ACHOQY_004192"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9680170.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3483..4112
                     /locus_tag="ACHOQY_004193"
     CDS             3483..4112
                     /locus_tag="ACHOQY_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9680171.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4251..5672)
                     /gene="melB"
                     /locus_tag="ACHOQY_004194"
     CDS             complement(4251..5672)
                     /gene="melB"
                     /locus_tag="ACHOQY_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP9680172.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5775..7130)
                     /gene="melA"
                     /locus_tag="ACHOQY_004195"
     CDS             complement(5775..7130)
                     /gene="melA"
                     /locus_tag="ACHOQY_004195"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9680173.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7413..8321
                     /gene="melR"
                     /locus_tag="ACHOQY_004196"
     CDS             7413..8321
                     /gene="melR"
                     /locus_tag="ACHOQY_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9680174.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLRQQRRQTFPG"
BASE COUNT         2149 a         2207 c         2057 g         1964 t
ORIGIN      
        1 acaaaaaata gaccgataaa tatcaataag ataacagcaa acaaaacatt aacatctgcg
       61 cagtacaaac tataaaccca tcgccagaga gtttttctct ctgaaaaagc cgcttatcac
      121 agtgcataaa tttgccgctg ctttaatcag ccaatattta ctgtgaggta tttgctaaag
      181 ccggtaacga ccaaacggat atttagtcag gctctgaaaa cagttcatac aaaacagaac
      241 gtgactgtga tctattcagc aaaaatttaa ataggattat cgcgagggtt cacacatgtt
      301 atttactatc caacttatca taatactgat atgtctgttt tatggtgcca gaaagggtgg
      361 tatcgcgctg ggtttattag gcggtatcgg tctggtcatt ctggtcttcg tcttccacct
      421 tcagccaggt aaaccaccgg ttgatgtcat gctggttatc attgcggtgg tggcggcatc
      481 ggcgaccttg caagcttcgg gcggtcttga tgtcatgctg caaattgccg agaagctgct
      541 gcgccgcaac ccgaaatatg tctcaattgt cgcgccgttt gtgacctgta cactgaccat
      601 tctttgcggt acgggtcatg tggtttacac cattctgccg atcatctacg acgtcgccat
      661 taagaacaac atccgtccgg aacgtccgat ggcggcaagt tctatcggtg cacagatggg
      721 gattatcgcc agtccggtgt cggttgcggt cgtgtctctg gttgcaatgc tgggtaatgt
      781 cacctttgat ggtcgccatc ttgagttcct cgacctgctg gcaatcacca ttccatcgac
      841 gttaatcggt atcctggcga tcggtatctt cagctggttc cgcggtaaag atctggataa
      901 agacgaagag ttccagaaat tcatctccgt accggaaaac cgtgagtatg tttacggtga
      961 taccgcgacg ctgctcgata aaaaactgcc gaaaagcaac tggctggcaa tgtggatttt
     1021 cctcggtgca atcgctgtag tcgcccttct tggtgctgat tcggacctgc gtccatcctt
     1081 cggcggcaaa ccgctgtcga tggtactggt tattcagatg tttatgctgc tgaccggggc
     1141 gctgattatt atcctgacca aaaccaatcc cgcgtctatc tcaaaaaacg aagtcttccg
     1201 ttccggtatg atcgccatcg tggcggtgta cggtatcgca tggatggcag aaaccatgtt
     1261 cggtgcgcat atgtctgaaa ttcagggcgt actgggtgaa atggtgaaag agtatccgtg
     1321 ggcgtatgcc attgttctgc tgctggtttc caagtttgta aactctcagg ctgcggcgct
     1381 ggcggcgatt gttccggtcg cgctggcgat cggcgttgat ccggcataca tcgtggcttc
     1441 agcaccggct tgctacggtt attacatcct gccgacttat ccgagcgatc tggcagcgat
     1501 tcagtttgac cgttccggca ccacccacat cggtcgcttc gtcatcaacc acagttttat
     1561 tctgccgggg ttgattggtg tgagcgtatc gtgcgtcttc ggctggatct tcgccgcgat
     1621 gtacgggttc ttataaatgc actttgcgtg ccgcccgtga ctacgcggca cgccattttc
     1681 gaataacaaa tacagagtta caggctggaa gctatgtcaa acaaaccctt tatctaccag
     1741 gcacctttcc cgatggggaa agacaatacc gaatactatc tactcacttc cgattacgtt
     1801 agcgttgccg acttcgacgg cgaaaccatc ctgaaagtgg aaccagaagc cctgacccta
     1861 ctggcgcagc aagcctttca cgacgcttct ttcatgctcc gtccggcaca ccagaaacag
     1921 gttgcggcta ttcttcacga tccagaagcc agcgaaaacg acaagtacgt ggcgctgcaa
     1981 ttcttaagaa actccgaaat cgccgccaaa ggcgtgctgc cgacctgcca ggataccggc
     2041 accgcgatca tcgtcggtaa aaaaggccag cgcgtgtgga ccggtggcgg tgatgaagaa
     2101 gcgctgtcga aaggcgtcta taacacctat atcgaagata acctgcgcta ttcacagaat
     2161 gcggcgctgg acatgtacaa agaggtcaac accggcacta acctgcctgc gcaaatcgac
     2221 ctgtacgcgg tagatggcga tgagtacaaa ttcctttgcg ttgcgaaagg cggcggctct
     2281 gccaacaaaa cgtatctcta ccaggaaacc aaagccctgc tgactcccgg caaactgaaa
     2341 aacttcctcg tcgagaaaat gcgtaccctc ggtactgcag cctgcccgcc gtaccatatc
     2401 gcgtttgtga ttggcggtac gtctgcggaa accaacctga aaaccgtcaa gttagcaagc
     2461 gctcactatt acgatgaact gccgacggaa gggaacgaac atggtcaggc gttccgcgat
     2521 gtccagctgg aacaggaact gctggaagag gcccagaaac tcggtcttgg cgcgcagttt
     2581 ggcggtaaat acttcgcgca cgacattcgc gttatccgtc tgccacgtca cggcgcatcc
     2641 tgcccggtcg gcatgggcgt ctcctgctcc gctgaccgta acattaaagc gaaaatcaac
     2701 cgcgaaggta tctggatcga aaaactggaa cacaacccag gccagtacat tccacaagaa
     2761 ctgcgccagg ccggtgaagg cgaagcggtg aaagttgacc ttaaccgccc gatgaaagag
     2821 atcctcgccc agctttcgca atacccggta tccactcgtt tgtcgctcac cggcaccatt
     2881 atcgtgggcc gagatattgc acacgccaag ctgaaagagc tgattgacgc cggtaaagaa
     2941 cttccgcagt acatcaaaga tcacccgatc tactacgcgg gtccggcgaa aacccctgcc
     3001 ggttatccat caggttcact tggcccaacc accgcaggcc gtatggactc ctacgtggat
     3061 ctgctgcaat cccacggcgg cagcatgatc atgctggcga aaggtaaccg cagtcagcag
     3121 gttaccgacg cgtgtcataa acacggcggc ttctacctcg gtagcatcgg cggtccggcg
     3181 gcggtactgg cgcagcagag catcaagcat ctggagtgcg tcgcttatcc ggagctgggt
     3241 atggaagcta tctggaaaat cgaagtagaa gatttcccgg cgtttatcct ggtcgatgac
     3301 aaaggtaacg acttcttcca gcaaatcgtc aacaaacagt gcgcgaactg cactaagtaa
     3361 cctcttcggc ccagcgcctg gcagcatgct gccaggtgat ccccctgggc cacctctttt
     3421 gcgattgtaa tttcacgctt gctggtgaat agtcagtatt ttccctgact tgcgaactca
     3481 ccatgacccg tacactcaag ccgttaattc ttaacaccgg cgcactggcg ctaacgttaa
     3541 tcctgattta taccggcatt tcggcccatg acaaactcac ctggctgatg gaagtgacac
     3601 cggtgattat tgtcgtgccg ctactgcttg ccaccgctaa acgttatccg ttaacgccgc
     3661 tgctctatac gctcattttc tttcacgcca tcatcctgat ggtcggcggg caatacacct
     3721 acgcgaaagt ccccatcggt tttgaggtgc aggaatggct ggggttgagc cgtaatccgt
     3781 atgacaagct ggggcatttt ttccaggggc tggtgcctgc actggtggca cgagaaattc
     3841 tcgtgcgcgg gatgtacgtg cgcggacgta aaatggtggc gttcctggtc tgctgcgtgg
     3901 cgctggcgat aagcgcgatg tatgaattaa tcgagtggtg ggcggcgctg gcaatgggcc
     3961 agggagcgga tgattttctc ggcacccagg gcgaccagtg ggacacgcaa tccgatatgt
     4021 tctgcgcgct gcttggcgca ttaacgacgg tgatattcct cgctcgcttt cattgccgcc
     4081 agctacggcg ctttggcttg ataaccggat aacaacgtgc gctttgttga tgccggatgc
     4141 ggcgtgaatt ccttatccgg tctacaaaat ttgcaaatcc aaagcattac attaagacgt
     4201 ggcctgataa acatatcgca tcaggccatt ttcgttagct attgtccacg tcaggctttc
     4261 acatcactca ctgcaccgac cggaatatca gcatgaacag gctcgggcgg tactttgcga
     4321 tatttatcca gcagatgaat ctggatcctg cgcagcgtgt cgccattgag gcgatagaaa
     4381 cggaagtaga gaatcagcgt taccatgaaa aacagagttg gtagagcaat cataataaac
     4441 tgcataccta atagggcttg cgtagactgt tcaacattcg gtacatagcc aatcatccct
     4501 aacacaaccg caatgaaaaa agccgcaaag gctgaaccgc ccttcaccac catagtctgc
     4561 acggagtaag cgatactttc acagcgtacg tgcagtttat attcaccgta atcaacggta
     4621 tctgccacca tgatgacctg taatacccag aaaagcgccg ttcccacatt cagcaaaata
     4681 cccgcaatca caatgaggac gacgttgtga tagctcatta atgccattaa caggagaaca
     4741 ccacagctta acaccggaag aatagatgct ccggcccata aaatgcgtcg ggataatgat
     4801 ttaactaagc gtgggaagaa tactaacgtc accaggttag cagctcccgc atacgacaga
     4861 taatagggga acaaatccgc atcaccgata acatatgaga aataatagat agcaaatccg
     4921 gtaataatgt tgctggctac attataagca agagccatac ccaagaggca tgaaagctga
     4981 tcgtttttat aaattagcgc aacgatggct ttaagtgtca gatggcttcc ttcagcagac
     5041 ggttgattgt ctgacgaaaa gacttcatgc acattgcgca gagtgatgat ggttgaaaca
     5101 ataaaaaagg cgattagtac cagagtgaac atctgaaagc caaatccccg atcgccaccg
     5161 ccgacataat taacaaatgg tagcgtcaca cctgccgtaa caaagcctgc cagactggca
     5221 aaaaaacgcg gataaggaac cagttgttcg cgctcacgtt tatcgagcgt gatggttgga
     5281 accagcgacc agaagggaat atccataatg gtgtaagtca tgccccagag gatgtaggtc
     5341 acgcaaacaa agacaatctg agtagtacct tcaaacagat gcgcactaaa gaggagaaat
     5401 aagattacag agtttgccaa cgtaccgatc aggatccagg gtttgaactt accccatcgc
     5461 gatcgcgtag catttacaat ccatcccata atcggatcgt taatagcatc ccagatcctc
     5521 gccaccagaa acaaagtacc gaccaaaccc acagacagcc caacgacatc ggtgtagtaa
     5581 tacatgaggt acatatacac aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca
     5641 taactgagtt ttgtagtcat tgaaatgctc atagggtatc gggtcgctgt taccaacgcc
     5701 cgccttctgt tattggtatc gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag
     5761 cgtttagtcg cgttttaacg gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc
     5821 aggtcgtcaa caagagcata tatttcatca atgcccagca cggcagcagt atgcgggtcc
     5881 atcatcgcgg cgtggtaaac acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc
     5941 gtctgtacgt tgatgttggt ttgcatcagg gcggccagat gcgaaggtag cgtaccgact
     6001 ttggtcggct gaatgccatt agcatcaacc agacaggcta cttccacgca acatccttgt
     6061 ggcaggttat caatcaaacc atcgttacgg acgttgccgt aaatcacact cggctcgcca
     6121 gtccagatag cgttcatgat tgtgctggca tattcccgtg acggtttaat atcaatccgg
     6181 gaggcgtttt tatactcctc cagctcttta tgccagttcg ccagctgctc gacgcagcgt
     6241 ttcgggtact catccagcgg tactttataa cgctcaatca aatcctcacg acctggctta
     6301 ataaaccacg gtgtgtactc agcaaaatgt tctgacgatt ccgtgacgaa gtagcccagc
     6361 tttttgaaca tttcgtagcg cacaatattc tggcagcgag tattgccatg aatattcggc
     6421 ttcggtgcct gccctgcttc ataagccgcc agcagttccg ggtagagatt cacataactg
     6481 ccgtcggcgg ttttgcgctc cagctccagg taaaacgcca tatggttgat acctgcgcaa
     6541 cggtaacgca gcgtagctgg gtcgatattg aggtcacgcg ccagctcttc cgccgttccc
     6601 tgcaccgaat ggcacagccc gacctgtttg atatgcggat agcgggcata catcgcccag
     6661 gtattcatcg ccattgggtt aacatagttg agcatggtgg catcggggca gacttccgtc
     6721 atgtcctcgc aaatttgcca cagatgcgga atggtacgta gcgcgcgcat aataccgccc
     6781 ggccccaacg tatcggcaat ggtttgttcc agaccatgcc gcttacagac ctcgaaatca
     6841 gtcaccgtgc aaggttcata accgccaatc tgaaatgcca ccacgacaaa atcggcatca
     6901 tgtaaggctt ctttctgttg ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc
     6961 ataagcttac gcaccacaat atgcgactct tccaggcggg tgggatcaat gtccatcagg
     7021 gcaatatgcg ccgttttcag cgcctcgcga tggaacacat caccaagaat atttttaacg
     7081 aaaatcgtcg aaccagcgcc gataaatgta attttgggtg cagtcatcat gcagatctcc
     7141 tggcttgctt gaataacttc atcatggcag gcgaatctgc agaaaacctc cggcttccag
     7201 gttgagcatt cccataaact cagatcctgc gtgaagcagc agtaaatctg agtttatggt
     7261 aataagttac ctgaaaagca gagttttcgg cctcatgatg ctcggacgct cccctcgcct
     7321 ctctgggaag agggtgaaaa ccgtgctccc actcgcagtc atcctccctc actcctgcca
     7381 taattctgat attccaggaa agagagccat ccatgaatac agatacgttt atgtgcagca
     7441 gcgacgaaaa acagacccgc agcccgctgt cgctgtattc ggaataccag cggatggaga
     7501 ttgagtttcg cgcaccgcat atcatgccca ccagccactg gcatggtcag gtcgaagtga
     7561 atgtgccttt cgatggcgat gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc
     7621 agggtcatat cacgctgttc tgggcctgta caccgcacca actaacagat accggaacct
     7681 gtaagagcat ggcgattttt aatctgccga tgcatctgtt tctctcctgg ccgctggata
     7741 aagacctgat taaccacgtc actcacggca tggtgatcaa atcactggcc acacagcaac
     7801 ttagcccgtt tgaagtgcgc cgctggcagc aggaattaaa cagtccgaac gagcaaattc
     7861 gccagctcgc cattgatgaa attggcctga tgctcaagcg atttagcctc tctggctggg
     7921 aaccgattct ggtcaataaa acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc
     7981 aattttatgt tagccagatg ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca
     8041 tcaacgatgt ggctgagcac gtcaaactta acgccaacta tgcaatgggg atattccagc
     8101 gggtcatgca attgacgatg aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg
     8161 cgttactgag cgataccgat aaaagtattc tcgatattgc cctgacggca ggctttcgtt
     8221 caagtagtcg tttttacagc acgttcggca aatatgtcgg catgtcgccg caacaatacc
     8281 gcaaacttcg ccaacagcgc cgccagacgt ttcccggcta aagacgatat cagtatcagc
     8341 caaaaaaata gtttagccgt cgataataag cagtgaa
//
LOCUS       DAZKGS010000184         8054 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKGS010000184 DAZKGS010000000
VERSION     DAZKGS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            337..1365
                     /gene="torT"
                     /locus_tag="ACHOQY_004197"
     CDS             337..1365
                     /gene="torT"
                     /locus_tag="ACHOQY_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9680175.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(1338..2030)
                     /gene="torR"
                     /locus_tag="ACHOQY_004198"
     CDS             complement(1338..2030)
                     /gene="torR"
                     /locus_tag="ACHOQY_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9680176.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            2180..3352
                     /gene="torC"
                     /locus_tag="ACHOQY_004199"
     CDS             2180..3352
                     /gene="torC"
                     /locus_tag="ACHOQY_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9680177.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3352..5906
                     /gene="torA"
                     /locus_tag="ACHOQY_004200"
                     /pseudo
     CDS             3352..5906
                     /gene="torA"
                     /locus_tag="ACHOQY_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            5903..6502
                     /gene="torD"
                     /locus_tag="ACHOQY_004201"
     CDS             5903..6502
                     /gene="torD"
                     /locus_tag="ACHOQY_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9680178.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(6595..6900)
                     /gene="cbpM"
                     /locus_tag="ACHOQY_004202"
     CDS             complement(6595..6900)
                     /gene="cbpM"
                     /locus_tag="ACHOQY_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9680179.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(6900..7820)
                     /gene="cbpA"
                     /locus_tag="ACHOQY_004203"
     CDS             complement(6900..7820)
                     /gene="cbpA"
                     /locus_tag="ACHOQY_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9680180.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         1941 a         2005 c         2199 g         1909 t
ORIGIN      
        1 taggcatagc cataatgcat gatggtgtgg cgtggcaaag cagagatata caaagtctgc
       61 cacatgtttt tatgacaaaa aattagcgca agaagacaaa aaatcacctt gcgctaatgc
      121 tctgttgtag gtcactaacc acatctaact cattgattaa tagtgactct agatgtagtg
      181 ttttatcgta ttttgcagtc tgttatttaa tcatgaattc atgtaacaat atgattttta
      241 aaatatttgt gtgtatttgt catcctttag gtgaaaaagg ttgagtcgca aagcggaatg
      301 catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct atttttactt
      361 ctttcccttt tcatgttatc ggcattttcg gctgataacc tgttgcgctg gcatgatgcg
      421 cagcattaca cggtgcaagc ctctacgccg cttaaagcca aacgcgcatg gaaactgtgc
      481 gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg tatgcaggag
      541 gcagctcgcc gctatggtgt ggatttaaaa gtgctggagg caggcggcta cagccagttg
      601 gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc cattttgctc
      661 ggtagtagca ctacgtcatt tcccgacctg caaaagcagg tagcaaatct gccggtgatc
      721 gaactggtaa atgctcttga tgatcccctg gtgaaaagcc gcgttggtgt accctggttt
      781 cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa accactgaac
      841 gtgctgttga tgcccggacc cgataatgcc gggggcagta aagagatggt cgagggtttt
      901 cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg tgataacgat
      961 attgaaatcc agcgtaacct gttgcaggag atgctggaac gccacccaga aatcgacgtc
     1021 gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa cctgaaaacg
     1081 ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct gaagcgggga
     1141 agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc ggttgagcag
     1201 gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc gattttagtt
     1261 ctgacgccga aaaatgccga ccgtgaacat attcgccgct cgctgtcacc agggggattt
     1321 cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc accatgttgc
     1381 gtcaccagta aatccgcgct gagtttatga cgtaaacgac gaattaacac atcgacggtg
     1441 cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gtagcagacg ttcacggctg
     1501 agaatttcgc ccggattcgt cacaaatgcc accaacattt catactctgc gcgggtcagt
     1561 ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca ataaccggca
     1621 aagcgatagc agttgtcctg agtgtgcggt tgagcttgtc gcgcgaggtc gattcgccag
     1681 agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt gacgtaatcg
     1741 tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc ggtaaccaga
     1801 ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc attttcatcg
     1861 ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat ttcccgcagc
     1921 ccggcaccgc tcgctgtaac ggaaacggta tacccctcct gagtgaagta agattgtaat
     1981 cgcgcctggg taaccggctc atcttcaaca ataacaatgt gatgtggcat cagagggttt
     2041 tactcattct gttcatatct gttcatatct gttcatatct gttcatattc tgccgtaagc
     2101 cgttcatcct gaccagtgcc gctgttcata tttgctcatt aagatcgctt cactaaacca
     2161 taattttaca ggggctatta tgcggaaact ctggaacgcg ctacgccgac ccagtgctcg
     2221 ttggtcggta ctggcgctgg tcgcaattgg gattgtgatt ggcattgcgc tgattgtatt
     2281 gccacacgtt gggatcaaag tcaccagcac aaccgaattt tgtgtcagtt gccacagtat
     2341 gcaaccggtg tatgaagaat ataaacagtc ggtgcatttc cagaacgcct ccggcgtgcg
     2401 agctgaatgc catgactgtc atatcccgcc ggatattcca ggcatggtga agcgcaaact
     2461 ggaagcgagc aatgatatct accagacctt tattgctcac tccattgata cacctgaaaa
     2521 attcgaagcc aaacgcgcgg aacttgccga gcgtgaatgg gcgcgaatga aagaaaacaa
     2581 ctcggcaacc tgccgctcct gccataacta cgatgcgatg gatcatgcga agcagcatcc
     2641 tgaagcggca cgtcagatga aggtggcagc gaaagataat caatcctgca tcgactgtca
     2701 taaaggtatt gcccaccagt taccggatat gagtagcggc ttccgtaagc agttcgatga
     2761 gctgcgcgcc agtgctaatg acagtggtga cacgctgtac tctattgata ttaagccaat
     2821 ttatgcggcg aaaggcgata aagaagcctc tggttctctg ctgcctgctt cggaagtgaa
     2881 agtccttaaa cgtgacggcg actggctgca aattgaaatt accggctgga cggaaagcgc
     2941 cggacgtcag cgtgtactca cccaattccc aggtaaacgc atctttgttg cctcgattcg
     3001 tggtgatgtg cagcagcagg taaaaacgct ggagaaaacc accgttgccg acaccaatac
     3061 cgagtggagc aagttgcagg ccactgcgtg gatgaagaaa ggcgacatgg tgaacgatat
     3121 caaaccgatc tgggcttatg cggattcgtt gtacaacggc acctgtaacc agtgccacgg
     3181 tgcaccggaa atcgcccact ttgacgctaa cggttggatc ggcacgctca acggcatgat
     3241 tggctttacc agtctcgata aacgtgaaga acgcaccttg ttgaaatatc tgcaaatgaa
     3301 tgcgtctgac accgcaggta aggctcacgg cgataagaag gaagaaaaat aatgaacaat
     3361 aacgatctct ttcaggcatc acgtcggcgt tttctggcac aactcggcgg cttaaccgtc
     3421 gccgggatgc tggggccgtc attgttaacg ccgcgccgtg cgactgcggc gcaagcggcg
     3481 actgacgctg tcatctcgaa agagggcatt cttaccgggt cgcactgggg ggctatccgc
     3541 gcgacggtga aggatggtcg ctttgtggcg gcaaaaccgt tcgaactgga taaatatccg
     3601 tcgaaaatga ttgccggatt gccggattgc cggatcacgt acacaacgcg gcgcgtattc
     3661 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctcagcgat acctcccagc
     3721 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     3781 aactggaacg cgtgcagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     3841 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     3901 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     3961 gcgtggttgg ctcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     4021 acagcaaaac cattgtgctg tggggctctg atttgctgaa aaaccagcaa gcgaactggt
     4081 ggtgcccgga tcacgatgtt tatgaatatt acgcgcagct aaaagcgaaa gtcgccgccg
     4141 gtgaaattga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     4201 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga tgtgccgctg caactggcgc
     4261 tggcgtatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     4321 tgggttttga gcagttcctg ccgtacctgc tgggtgagaa agacggtcag ccgaaagatg
     4381 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     4441 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     4501 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     4561 caggtggtgg ctttggtttt ggctggcact ataacggcgc aggcacgccg gggcgtaaag
     4621 gcgttattct gagtggtttc tccggctcta cgtcgattcc gcctgttcac gacaacagtg
     4681 attacaaagg ctacagcagc accattccga ttgcccgttt tatcgatgcg atcctcgaac
     4741 cggggaaagt gatcaactgg aacggtaaat cggtaaaact gccgccgctg aaaatgtgta
     4801 tttttgccgg aactaaccca ttccatcgcc atcagcagat caaccgcatt attgaaggct
     4861 ggcgcaagct ggaaacggtt atcgccatag ataaccagtg gacctcaacc tgccgctttg
     4921 ccgatatcgt gctgcctgcg accacgcagt ttgagcgtaa cgatctcgac cagtacggca
     4981 accactccaa ccgtggcatt atcgccatga aacaggtggt gccgccgcag ttcgaggcgc
     5041 gcaacgactt tgatattttc cgcgagctgt gccgtcgctt taatcgcgaa gaagccttta
     5101 ccgaagggct ggacgaaatg ggctggctga aacgcatctg gcaggaaggt gtacaacaag
     5161 gcaaaggacg cggcgttcat ctgccagcgt ttgatgactt ctggaataac aaagagtacg
     5221 tcgagtttga ccatccgcag atgtttgttc gccaccaggc attccgcgaa gatccggatc
     5281 tcgaaccgct gggcacgccg agtggcctga ttgagatcta ctcgaaaacc atcgccgata
     5341 tgaactacga cgattgtcag gggcatccga tgtggtttga gaaaatcgaa cgctcccacg
     5401 gtgggcccgg ctcgcagaag tatccgttgc atctgcaatc tgtgcatccg gatttccgac
     5461 ttcactcgca gttatgtgag tcggaaactc tgcgtcagca atatacggta gcgggtaaag
     5521 agccagtgtt cattaacccg caggatgcca gcgcgcgcgg tattcgtaac ggtgatgtgg
     5581 tacgcgtctt taacgctcgc ggtcaggtgt tggcaggggc agtagtttct gaccgctatg
     5641 cacccggcgt ggcacgaatt cacgaagggg catggtacga tccagataaa ggcggcgagc
     5701 ctggtgcgct gtgcaaatac ggtaacccca acgtgctgac catcgacatc ggtacttcgc
     5761 agctggcgca ggcgaccagt gcgcacacta cgctggtgga aattgagaag tacaatggag
     5821 cagtggagca ggtgacggcg tttaacggcc ctgtggagat ggtggcgcag tgcgaatatg
     5881 ttcccgcgtc gcaggtgaaa tcatgaccac gctgacagca caacagattg cctgtgttta
     5941 cgcctggctg gcgcagttgt tctcccgtga actggacgat gaacaactga cgcaaatcgc
     6001 cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc tggctgcggc
     6061 ggtgaatgag ctggaaaact gtattgccac gctgacagta cgtgacgatg cccgtctgga
     6121 actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg cgctgccgta
     6181 tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg aggcagggat
     6241 ggaaaccagc ggcaatttca acgaaccggc agatcatctg gcgatctatc tcgaattgct
     6301 cagccatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa tcgacagttt
     6361 gcggcaaaaa acactgacgg cgctgtggca atggttacca gagtttgttg tgcgttgtcg
     6421 tcagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg tgttagtgga
     6481 gagcgaccac caaaacagat aacgtcgttt gtgcgcctga aaagacgcgt ttagcgtcgc
     6541 atcaggcatt atggggcagt tgccggatgc gtcatcacat ccggcaaaca cacatcacgg
     6601 atgagttaca aaccgggaaa gtcgctggcg cagcaggcgg ttttcctgct tcaggtgcgc
     6661 aatatcatcc attaacgtca gcgccaccgc gatcccaggc cagtccagag ccagttcatg
     6721 acgcaggcgt actgcgcgtt gcaccacgat ggcggcatgg tcgtcaaata cccaggttgt
     6781 ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca actcctcttc
     6841 agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa cattagccat
     6901 tatgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc cagttgctgc
     6961 cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag taccgcatac
     7021 agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa tcgttgcccg
     7081 gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg aacggtgact
     7141 ttagcaccca gcgccgcttc ccacgggcta accggcacta caatttccag atcatggccg
     7201 acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc gccatttgga
     7261 ccgccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc attgccgacg
     7321 cccgccggga tcttgacatt cagcgttttc ggaatttcct gttcgatcat gccaaaggcg
     7381 ttataaaccg gcaggttata gctgatggta cgcttatgct cagtaagcgt ttcttcgagg
     7441 aataccgcca cttcgatttc aatatcgtgg ccgcgtgtgg cggggcgttg acggctctgg
     7501 cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc gttaaaactc
     7561 tgaccgtcgc tatgatggaa ctgacggtta aattgcggat cgttgcgatg ttgccacatc
     7621 tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc agcgacctct
     7681 ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt gcgggccagt
     7741 cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc catgatggcg
     7801 taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt gaaggaaccc
     7861 ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc caacacagaa
     7921 tatgttattg aaatcatcgc ggagaggagg tcgcctcaag gtggggtgct gaacatattt
     7981 taaacaggtg aaaaagggtg agcgattttt gatagtggaa ccaggcactt taagtttaac
     8041 tggggcgtca ttat
//
LOCUS       DAZKGS010000185         7985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKGS010000185 DAZKGS010000000
VERSION     DAZKGS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..434)
                     /gene="cedA"
                     /locus_tag="ACHOQY_004204"
     CDS             complement(171..434)
                     /gene="cedA"
                     /locus_tag="ACHOQY_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9680181.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            617..2878
                     /gene="katE"
                     /locus_tag="ACHOQY_004205"
     CDS             617..2878
                     /gene="katE"
                     /locus_tag="ACHOQY_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9680182.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3137..3895)
                     /gene="chbG"
                     /locus_tag="ACHOQY_004206"
     CDS             complement(3137..3895)
                     /gene="chbG"
                     /locus_tag="ACHOQY_004206"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9680183.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3908..5260)
                     /gene="celF"
                     /locus_tag="ACHOQY_004207"
                     /gene_synonym="chbF"
     CDS             complement(3908..5260)
                     /gene="celF"
                     /locus_tag="ACHOQY_004207"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9680184.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5365..6207)
                     /gene="chbR"
                     /locus_tag="ACHOQY_004208"
     CDS             complement(5365..6207)
                     /gene="chbR"
                     /locus_tag="ACHOQY_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9680185.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6215..6565)
                     /gene="chbA"
                     /locus_tag="ACHOQY_004209"
     CDS             complement(6215..6565)
                     /gene="chbA"
                     /locus_tag="ACHOQY_004209"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9680186.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6616..7974)
                     /gene="chbC"
                     /locus_tag="ACHOQY_004210"
     CDS             complement(6616..7974)
                     /gene="chbC"
                     /locus_tag="ACHOQY_004210"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9680187.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2148 a         2080 c         1786 g         1971 t
ORIGIN      
        1 aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagatcactt atttatagca
       61 atagatcgtc aaaggcagct ttttgttact ggtggtttga atgaatgtag caatgaaata
      121 cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg tacgtaatct ttactcagtc
      181 acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg cacagtaaac
      241 gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt gccacgcagc
      301 atccagacat cacgaaaaga atccatcttt atcgcatgtt ctggcggcgc gggttccgtg
      361 cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt cattactggc
      421 ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta gcccctgtac
      481 gtcccacttt gcgtgtattt cataacaccg tttccagaat agtctccgaa gcgggatctg
      541 gctgatggtc tatagttaga gggttttttg accaaaacag cggccctttc agtaataaat
      601 taaggagacg agttcaatgt cgcaacataa cgaaaagaac ccacatcagc accagtcacc
      661 actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg aggacggatc
      721 tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc ctgggagcct
      781 gaaagcccct gatacacgta acgaaaaact taattctctg gaagacgtac gcaaaggcag
      841 tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc aaaactcact
      901 gcgtgccggt aaccgtggtc caacgctgct ggaagatttt attctgcgcg agaaaatcac
      961 ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat cagcagctca
     1021 cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt tcctctcaga
     1081 tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg gcgctggctc
     1141 tgccgatacc gtgcgtgata tccgtggttt tgccaccaag ttctatactg aagagggtat
     1201 ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc ataaattccc
     1261 cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag ggcaaagtgc
     1321 ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca acgtgatgtg
     1381 ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct tcggtattca
     1441 caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc actggaaacc
     1501 actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg gacgtgaccc
     1561 ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc cggaatacga
     1621 actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg atcttctcga
     1681 tccaaccaaa ctgatcccgg aagaactggt gcccgttcag cgtgtcggca aaatggtgct
     1741 caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc atccaggtca
     1801 tattgttcct ggtctggact tcaccaacga tccgctgttg cagggacgtt tgttctccta
     1861 taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc cgattaaccg
     1921 tccgacctgc ccttaccaca acttccagcg tgatggcatg catcgtatgg ggatcgacac
     1981 taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg aaacaccgcc
     2041 gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa ataaagttcg
     2101 tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg cgtctgttct ggctaagtca
     2161 gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa gcaaagtcgt
     2221 tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc tcactctggc
     2281 ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga atatcacccc
     2341 acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg ccattcctga
     2401 cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga gatcggcaga
     2461 ccttctggcc attctcaagg ccctgaaggc caaaggcgtt catgccaaac tgctctactc
     2521 ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg ctacctttgc
     2581 cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata tcgcggatat
     2641 cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc ttaaaccgat
     2701 tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg accagggtga
     2761 agaagggatt gtggaagctg acagcgctga cggtagtttt atggatgaac tgctaacgct
     2821 gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc ctgcctgatg
     2881 ggaggcggcg caattgcgcc gcctcaaatg atttacatag tgcgctttgt ttatgccgga
     2941 tgcggcgtga acgccttatc cggcctacaa agtcgtataa attcaatgta ttgcagttta
     3001 ttggtagata ctgataagcg aagcgcatca ggcagttttg cgtttgtcag cagtctcaag
     3061 cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga tgcaaggttc acgccgcatc
     3121 tggcaaacat cctcacttac acatcccgat aactccccaa ccgataacca cgctgagcga
     3181 tagcaccttt caacgacgct gatgtcaaca catccagctc cgttaagcgt gggaaacagt
     3241 aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc tccagcgaac
     3301 gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg ctaatctctt
     3361 caccataaaa cgcgctactg aatccctgag aactgcgcag attagccggt aaatcaccag
     3421 cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc gcaaacctgg
     3481 caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca agatgcgtag
     3541 gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc tgagtaattt
     3601 cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc agcacaccat
     3661 cgcgggttaa ccccggcata gctgtcaggg gcttgcccat agtaaggaca aagtgcatcc
     3721 ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata gcctgcccat
     3781 tcaccagcgc cgtcgttgat gtgacaatcc cattgcgaca ggcctcgata atgccgtagt
     3841 tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt tccataatca
     3901 gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt ggcagccatt
     3961 tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg gaatgcacca
     4021 acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt gcggcgttgc
     4081 tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta tcatcgaaat
     4141 gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag cttacaggtc atttctaccg
     4201 cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg taatgttcag
     4261 cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag taataagccc
     4321 caccgcgctg ttccagttct ttcggcttaa ctttcaactc cggattttta tacagctcaa
     4381 aaagttgttt ctcgactttc tgtactacct gtgctcgtgc gccgcctttg tagtattcgc
     4441 ccatttcaat agccagcatc tctttctgct tgaagtaata cagcagataa gaacatggca
     4501 gcagatttaa cgaacgaatt aagccctcac taaatggcag atcgaaaata ttttttacag
     4561 aggacgcttt taactgccct gacgccacac catcaagcaa ttcggcaaag cgcgacttgc
     4621 catttaccag cacatcctta atgaacacca tatggttgag gccgaacaga tcgatagata
     4681 aatcatcgct gtctttcagc atcagaacat cgcgaataaa catcttcatg ccgatcggaa
     4741 tattacacac gccgataaag cgtttaaatc cggtatgacg ataaacggct tcagtgacca
     4801 ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct tcgacatctt
     4861 ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacagaccg cccgcgccgt
     4921 tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt tcgcgcgccg
     4981 gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc atctttcaat gcttcgcggc
     5041 gatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc cgttggcaga
     5101 gttcaaaaat gatatccagt ttctctttac catcttcgac atccaccagc cataattcgc
     5161 tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg gtatagctgc
     5221 tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc ttcagtacag
     5281 aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa caggacagat
     5341 atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct tacgatagct
     5401 cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg ggctactata
     5461 accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt gttttttggc
     5521 aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat atcgttgagt
     5581 ggctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct ccagcgccga
     5641 ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc actgcggtac
     5701 atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat aaaaagtaac
     5761 catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa tcactgtttc
     5821 cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcgacaa agcaataagg
     5881 caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt tcaatatacg
     5941 cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa aaacaaaatc
     6001 gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc cggttaatac
     6061 cagagtaaat tcataatagt cgtgctgatg cagcccgctg atactctcag ttttgttata
     6121 gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag tggcaatttc
     6181 cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt tcatgaagct
     6241 caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga tcctgagcgt
     6301 ggaccagcac cagactcact ttcatcttaa cttcgcccgc atcgccttca atcagtttcg
     6361 tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg gcttttgctg
     6421 cagcaaaatc gccctgcttc gcctgtttca gtgccgcata cgccaggctg cgcgcttgtc
     6481 cggagttgat gataagcccc atcaccactt cttccagctc ttcagcttct gtttgcgtat
     6541 caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg gcaagattac
     6601 ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc tctcttcttt
     6661 atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa ttaaggttgc
     6721 gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg tgttaaagaa
     6781 ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag gaatgatgcc
     6841 catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta caaacgggat
     6901 aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt taatctggaa
     6961 gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc gagaggcgat
     7021 aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa taaaggagtc
     7081 cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg cttcgacgga
     7141 accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa tgccgttgtc
     7201 cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg gaacaaagat
     7261 cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga tggtatccat
     7321 aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa tccccatcac
     7381 ggaaagaata ataaagccgg gaattaatgc cgagaaggaa cgcgatactg aagcaggtac
     7441 gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca tttctgccac
     7501 caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt ttgcgccaac
     7561 cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa cggataacaa
     7621 ccccgccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa taaagaacgg
     7681 tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa tacctttcag
     7741 gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga
     7801 cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc ccgcaagggt
     7861 taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct gctttcctat
     7921 tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac tcatagaaaa
     7981 atacc
//
LOCUS       DAZKGS010000186         7950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKGS010000186 DAZKGS010000000
VERSION     DAZKGS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..282)
                     /locus_tag="ACHOQY_004211"
     CDS             complement(40..282)
                     /locus_tag="ACHOQY_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9680188.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDAEVIIKSTS
                     NNGLSVTRTADKDSAKTFVQETLKDTRESADEWFVR"
     gene            414..1697
                     /gene="ydfJ"
                     /locus_tag="ACHOQY_004212"
                     /pseudo
     CDS             414..1697
                     /gene="ydfJ"
                     /locus_tag="ACHOQY_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1786..3246
                     /gene="ydfI"
                     /locus_tag="ACHOQY_004213"
     CDS             1786..3246
                     /gene="ydfI"
                     /locus_tag="ACHOQY_004213"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9680189.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3282..3485)
                     /gene="ydfZ"
                     /locus_tag="ACHOQY_004214"
     CDS             complement(3282..3485)
                     /gene="ydfZ"
                     /locus_tag="ACHOQY_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9680190.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3663..4349)
                     /gene="rspR"
                     /locus_tag="ACHOQY_004215"
                     /gene_synonym="ydfH"
     CDS             complement(3663..4349)
                     /gene="rspR"
                     /locus_tag="ACHOQY_004215"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9680191.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4438..5184)
                     /gene="ydfG"
                     /locus_tag="ACHOQY_004216"
     CDS             complement(4438..5184)
                     /gene="ydfG"
                     /locus_tag="ACHOQY_004216"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9680192.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5321..7366
                     /gene="dcp"
                     /locus_tag="ACHOQY_004217"
     CDS             5321..7366
                     /gene="dcp"
                     /locus_tag="ACHOQY_004217"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9680193.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7411..7929)
                     /gene="ydeJ"
                     /locus_tag="ACHOQY_004218"
     CDS             complement(7411..7929)
                     /gene="ydeJ"
                     /locus_tag="ACHOQY_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP9680194.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1984 a         1843 c         2017 g         2106 t
ORIGIN      
        1 attgaatgat ttccagccgt tactgatttt actcgttaat tagcgaacaa accactcgtc
       61 agcagactcc cgggtatctt tcagcgtctc ctgaacaaaa gtttttgcag aatctttatc
      121 ggcggtgcga gtaactgaaa ggccattgtt gctggtggat ttgatgatca cttctgcatc
      181 gtcataacgt ttactgacac gtcgcagcat ttcttgttgc agggcaggaa cagaaccttt
      241 tggcattttg ttgattttgg ctgcggggta tctgtggggc atatatggga cataaaaagg
      301 cctcaaataa acagcaaaat cgagattagg gatttttaga aaaaatacaa ccatctgaaa
      361 aaacttagaa aacacccaaa aacccacaga atagtaaaaa acatctatga attatggatt
      421 tccagttata ttcgctcggc gcagcgttag tgtttcatga aatatttttt cctgaatcat
      481 caacggcaat ggcgttaatt ttggcaatgg gaacctacgg tgcaggttat gtggcgcgta
      541 ttgtcggagc atttattttc ggcaaaatgg gcgacagaat agggcgtaaa aaagtgctct
      601 ttattaccat caccatgatg gggatctgta ccaccttaat tggtgtgtta ccgacctatg
      661 cacagattgg tgtttttgcc cccatcttgc tggtgacgct gcgtattatt caggggttgg
      721 gtgcaggtgc ggaaatttcc ggtgccggta cgatgctggc ggaatatgcg ccaaaaggta
      781 agcgcggaat tatctcctca tttgtagcta tgggaactaa ctgcggaacc ttgagcgcaa
      841 cggcaatctg ggcctttatg ttcttcattc tcagtaaaga ggaactgctt gcgtggggat
      901 ggcgtatacc gttcctggcg agcgttgtcg tgatggtctt tgctatctgg ttgcgtatga
      961 atctgaaaga aagcccggtc tttgagaagg ttaacgacag caaccaaccc acagcaaaac
     1021 ctgcacctgc tggtagcatg ttccagagca aatccttctg gctggcaaca gggctgcgtt
     1081 ttggtcaggc gggtaactcc gggttaattc agactttcct tgcaggctat ttagtgcaga
     1141 cgttattgtt taacaaagca attccaacag atgcattgat gattagttcg attctcggct
     1201 ttatgaccat tccgttcctt ggttggttat ccgataaaat tggtcgccgg atcccgtata
     1261 ttattatgaa tacctccgcg attgtgctgg catggccaat gctttctatc attgtagata
     1321 aaagctatgc cccgagcacc attatggttg cactgattgt gattcataac tgtgcggtgc
     1381 tgggattatt tgctctggaa aacattacca tggcagaaat gttcggctgt aaaaaccgct
     1441 ttacccggat ggctatttct aaagaaattg gtggtcttat cgcttccggt tttggtccta
     1501 tcctggcggg tattttctgc accatgacgg aatcctggta tccgatcgcc attatgatca
     1561 tggcatattc agtaattggt ttaatctctg cgctgaaaat gccagaggtg aaagaccgtg
     1621 atttaagtgc gctggaagac gctgcggaag atcaaccgcg tgttgtaaga gctgcgcaac
     1681 cttccagaag tctgtaaatc ctcaatccct tctcttaccg gagaggggat ttttattcat
     1741 ataacaaaac atatagcttg ccatatttat atttaaggaa ttgttatggg aaataatttg
     1801 ttatcagcaa aagcgacact ccctgtttat gatcgtaata acctggcccc aagaattatt
     1861 catttaggct ttggtgcatt tcaccgtgcg catcagggcg tgtatgccga tattcttgct
     1921 acagaacatt tcagtgactg gggatattat gaggtcaact taatcggcgg cgaacagcaa
     1981 attgccgatt tacaacagca agataatctt tataccgttg cggaaatgtc ggccgatgcg
     2041 tggacggctc gcgtcgttgg cgtcgttaaa aaagccttgc acgtacagat ggatggctta
     2101 gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga ttgtctctct gacaatcacc
     2161 gaaaaagggt atttccactc tccggcgatc ggacagttaa tgctcgatca cccgatggtc
     2221 gctgccgacg tgcaaaatcc ccaccagccg aaaacagcaa caggagtgat tgttgaggcg
     2281 ctggcccgcc gtaaagcggc aggacttccc gcatttaccg tcatgtcatg tgacaacatg
     2341 ccagaaaacg gtcatgttat gcgtgacgtt gtcacttcat acgcgaaagc tgttgatgta
     2401 aaactggcac aatggatcga agataacgtg actttcccat caacaatggt ggaccgtatt
     2461 gtgcccgcag tgacagagga tacgctggcg aaaatcgaac aacttaccgg tgtgggcgat
     2521 cctgcgggcg ttgcctgtga acctttccgc cagtgggtaa tagaagataa ctttgttgcc
     2581 ggacgtccag aatgggaaaa agcgggagcc gaactggtta gtgatgtgct gccttatgaa
     2641 gagatgaagt tgcgcatgct caacggcagt cattcattcc tggcgtattt gggttatctt
     2701 gctggatatc agcacattaa tgactgcatg gaagatgaac attatcgtta tgcggcgtat
     2761 ggcttgatgt tgcaggaaca agcgccgacg ctgaaagtgc agggcgttga tttgcaagat
     2821 tacgctaacc gattaattgc acgctatagc aacccggcgt tacgtcatcg aacctggcag
     2881 attgcgatgg atggtagcca gaaattgcca cagcggatgt tggattctgt tcgctggcat
     2941 ctggcgcatg acagcaagtt cgatctgctg gcgctgggcg tcgcgggttg gatgcgttat
     3001 gtcggtggtg ttgatgaaca gggaaatctg atagaaatca gtgacccact gttacctgtt
     3061 attcagaagg ctgtacaaag tagtgccgaa gggaaagcgc gcgtccagtc attgctggcg
     3121 attaaggcaa tctttggtga tgatttgcca gacaatagct tgtttactgc aaaagtgacg
     3181 gaagcgtact tgtctttatt agcgcatggc gcgaaagcga ccgtggcgaa atattccgtg
     3241 aagtaaaaaa agcaatgccg ccatcggcgg cattcacaca cttagttcat gccgagacga
     3301 atcaggtcca gaggtgccag tttctcttca caaccttcaa caactacggt ttttccgcgg
     3361 cggatttgct cagcggtcag accttcagta tcaatcgaca ggatcttgcc agtgtgaccg
     3421 gtgccgctaa ccataacacg gctgccagtg gtgattgcgt tacggttacg atcgtaggtc
     3481 gtcataggtg ttttctcctt tctgatttac agtcgaagcc cacgtaacgg gcgcaatata
     3541 aatactcctg tcgagtgaaa tattttttgt ttttgatcaa gctcacatct tttttgatgc
     3601 atgcatgagc cgtaaggcgg ggggaaaagc ctctcatccc ggatgggatg agagtaaatg
     3661 aattactctt cgctaaacca gtcgctgttt tcctggcgga tctggcgcac ggattcgctg
     3721 atttcctgca aatgttgcgt cattgcacgt tctaccgcat cgccatcacg tttttgcagg
     3781 gcagagaaaa tatcaagatg ctggcgtaac agcatttctg gtggagaaac atggtcgaaa
     3841 ctcatatagc gcacgcgatc aacggtcgct ttcaggttct caatggtatc ccacgccagt
     3901 tgacagtcgg caatctgcgt caggagttga tggaagttgt catcaagttc aaaaaaatca
     3961 tccagttgct tgcgctcaat ggcaatgcgt tgctggtgaa gattttgttc cagttggtag
     4021 cactggcttt cggtaatcat gctcgccgcc cgacgcgcca ccgcgcactc aatggcctga
     4081 cggataaaac tgccgtttcg cacctgggcc atggaaattt tgttgacgta gctgccacgc
     4141 tgcggacgaa tttgaatcag gccgttttcc gccagtttaa taaaggcttc acgaaccggc
     4201 tggcgtgaca cattgaaacg aacagaaact tctttttccg acaacggtgt gcctggtgca
     4261 atcaggcaat ggacaatgtc gcgacgaaga atacgataaa tctgctgatt gacaggctgt
     4321 gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag ttaactgcat
     4381 gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt ataaaaatta
     4441 ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt ccagggtatt
     4501 gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga catcttctgg
     4561 cgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg
     4621 gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga cgcgcaccgc
     4681 cgtaccatgc aggtccgtac gcagattcag gctaaactga cgaacaaacg ctttcgtcgc
     4741 accgtaaacg ttaccaccgg cataaggcca gctacctgcc gttgagccaa tgttaataat
     4801 atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat ataccaggcc
     4861 tttgttgttg gtatcaatca tcgtttccca gtcttcaacg ctggctttat gcgcaggctc
     4921 catgcccaac gccaggccgg cattatttac caggatatca atattgcacc actcggcagg
     4981 gagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt gggcgatata
     5041 cagattatct cccagttcgt cttttaactc ctgcaaccgc tcctggcgac ggccagtggc
     5101 gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa aacctgccgt
     5161 tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt gtcaataaca
     5221 tagcacgtga tgcttaagag cgggtaatac tggtttgcaa gattgcactc cgttatcagt
     5281 aagcctacgc tatggaacac ctcgcgttca ggagaagaaa atgacaacaa tgaatccttt
     5341 ccttgtgcaa agcacactgc cgtatctggc tcctcatttt gatcaaattg ccaatcatca
     5401 ctatcgcccg gcattcgatg agggaatgca gcaaaagcgg gcagaaattg ctgccatcgc
     5461 gcttaacccg caaacgcctg atttcaacaa tactattctg gcactggaac aaagcggaga
     5521 attacttacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata ccaatgatga
     5581 attacagcgt cttgatgaac agttttccgc tgaactggcg gaactggcta atgatatcta
     5641 tctgaacggt gaattattcg cgcgggtaga tgctgtctgg cagcgccgtg aatccctggg
     5701 gcttgatagt gaatccatcc gcctggtgga ggtgattcat caacgttttg tccttgccgg
     5761 agccaaactt gcgcaagctg ataaagcaaa attaaaagta ctgaatacag aagctgcgac
     5821 actgaccagt cagtttaacc agcggttact ggcagcaaat aaatccggcg gtctggttgt
     5881 gaacgatatc gcgcagctgg caggaatgag tgagcaagag attgcgctgg cggcagaggc
     5941 ggctcgcgag aaaggtctgg ataacaaatg gctgattccg ctgctgaata ccacccaaca
     6001 accggcgctt gccgaaatgc gcgatcgtgc gacgcgtgaa aaactgttta ttgcgggctg
     6061 gacgcgagcg gaaaaaaatg atggcaatga tacccgcgct atcattcaac gtctggtgga
     6121 gatccgtgca caacaggcga cattacttgg ttttcctcat tatgccgcat ggaaaatcgc
     6181 cgatcagatg gcaaaaacac ctgaagcagc gctcaacttt atgcgggaaa ttgttccagc
     6241 ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttattg ataagcagca
     6301 gggcgggttt agcgcgcagc cgtgggactg ggcattttat gccgaacagg tacggcggga
     6361 gaaatttgat cttgatgagg cgcagctcaa gccatatttt gaattaaaca cggtgttgaa
     6421 tgaaggtgta ttctggaccg cgaatcagct cttcggtatt aagtttgtcg aacgttttga
     6481 tattcctgtc taccatcctg acgttcgtgt gtgggaaatt tttgatcata atggcgtggg
     6541 actggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg gtgcatggat
     6601 gggcaatttt gttgagcaat caacgcttaa tgaaacgcat tcggtaattt ataacgtttg
     6661 caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg atgtcataac
     6721 cttattccat gaatttggtc atacgctgca cggccttttt gcccgccagc gttatgccac
     6781 gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca
     6841 ctgggcaacg catccgcagg tattcgctcg ctacgcccgg cattatcaga gcggggcagc
     6901 aatgcctgac gaactgcaac agaaaatgcg taatgccagc ctgttcaaca aagggtatga
     6961 gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgcc tggaagaaaa
     7021 cgaagcaatg caggatgtcg atgattttga attgcgggcg ctggtggcgg aaaatatgga
     7081 tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt ttggtggcgg
     7141 atatgccgca ggttattacg cttatctgtg gacacaaatg ttggccgatg atggttatca
     7201 gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg ctgcgttttc gcgaggcgat
     7261 cctttccaga ggtaacagcg aagatctgga acgcctgtat cgacaatggc gcggtaaggc
     7321 tcctcagatt atgccgatgc tgcaacatcg tggcttgaac atataaacac caccaatggc
     7381 tgtcctggag atatcgccag gccagcctgg ttatagcagt aattgcagca gctgggcgag
     7441 ggcaaacctc accgctaaag ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc
     7501 agtataggtc tggcctttaa tatgccacgc gaaccagacg gtacccgctg gcgtaccatc
     7561 ttcaccgccc tccggtccgc cgtagccggt aatggcaata cttacatcag catccgcacg
     7621 ctctatggct ccggttgcca tttctgctgc cactttctca ctcaccgcag aatatcgttc
     7681 aagagattgc tggcttacgc tgaggatttt catctttgcc tgatcggtga aagtaacaaa
     7741 gcctgcaccg taaaatttgg gtgtatcttc agctgcacac agggcgctag ccaacttacc
     7801 gccggtgcat gattcggcgg tagttaagcg taattgatcc gcgataagtc tttgactcaa
     7861 cgcggatgtc aggttttcaa tagtgtcggt atctgcgagt tgtacaattt tatctttatt
     7921 aatgttcact gttttttgtc cacgctatta
//
LOCUS       DAZKGS010000187         7938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKGS010000187 DAZKGS010000000
VERSION     DAZKGS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(312..1343)
                     /gene="rsmC"
                     /locus_tag="ACHOQY_004219"
     CDS             complement(312..1343)
                     /gene="rsmC"
                     /locus_tag="ACHOQY_004219"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9680195.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1446..1859
                     /gene="holD"
                     /locus_tag="ACHOQY_004220"
     CDS             1446..1859
                     /gene="holD"
                     /locus_tag="ACHOQY_004220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9680196.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            1828..2274
                     /gene="rimI"
                     /locus_tag="ACHOQY_004221"
     CDS             1828..2274
                     /gene="rimI"
                     /locus_tag="ACHOQY_004221"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9680197.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2289..2966
                     /gene="yjjG"
                     /locus_tag="ACHOQY_004222"
     CDS             2289..2966
                     /gene="yjjG"
                     /locus_tag="ACHOQY_004222"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9680198.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3057..4646
                     /gene="prfC"
                     /locus_tag="ACHOQY_004223"
     CDS             3057..4646
                     /gene="prfC"
                     /locus_tag="ACHOQY_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9680199.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5039..5644
                     /gene="osmY"
                     /locus_tag="ACHOQY_004224"
     CDS             5039..5644
                     /gene="osmY"
                     /locus_tag="ACHOQY_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9680200.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5771..5932
                     /locus_tag="ACHOQY_004225"
     CDS             5771..5932
                     /locus_tag="ACHOQY_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9680201.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6054..7127
                     /locus_tag="ACHOQY_004226"
     CDS             6054..7127
                     /locus_tag="ACHOQY_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9680202.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7124..7906
                     /locus_tag="ACHOQY_004227"
     CDS             7124..7906
                     /locus_tag="ACHOQY_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9680203.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1897 a         2056 c         2151 g         1834 t
ORIGIN      
        1 gccaccttcg cataccatca attcttaaaa agaattgcta ccacggaggc gcattctagt
       61 ggttttcagc ttttcgtcaa tagttaatta tcgccggagg tgtaattgct ggaaaaatgt
      121 ccatcaggaa actaacgtgc aggttttgta ggtcggataa ggcgttcacg ccgcatccga
      181 cacggtattt gggtgcagat gccagatgcg acgcgggcgc gtcttatctg gcctacgaag
      241 ggctaacgtg caggttttgt aggttgtata aggcgagcac gccgcatccg acaaggcttg
      301 cggcgggata cttaaccttt cttcgcctgg cgggtcataa tggcgcgata caccttgaag
      361 cgcccggttt gcgcgataac ttcatggaag ccaaatgtct catccagcac gtccgggtaa
      421 ggcaggaagg cgttcgctac aattcgcagc tcgccgccgc tattaagatg acgcaccgcg
      481 ccgcgaatca gcgtttgcgc cgcatccagg ctggtttgca tcccatcgtg gaacggcggg
      541 ttggagatga tcatatcaaa acgacctttc acctcggaaa agacgttgct ggcaaagact
      601 tcaccttcaa caccgttggc cgcaagcgtc gcacggctgg cttctaccgc cggggcagag
      661 acatcgcaca aggtgagacg aattttcggc gaatggcgcg caaaggcaac tgaaagcaca
      721 cccgcgccac agccgacatc cagcacttta cctttcgtgt gcggggttaa cgtcgagagc
      781 agcaactggc taccgacatc cagaccgtcg cggctaaaca cgccaggcag cgttttgacc
      841 gtcaggccat cgacgctgta ttcgccccag aatttatccg catcaaatac tggctgtttt
      901 tccagacgac caaaatagag gccacagcga cgagcgctgt cgactttatt caacggcgca
      961 taatctgcca gcatctgctc ggcgctgcgc acgccgctgc ggttctcgcc aacgacaaaa
     1021 atatccgtcc ccaccggcag cagagaaagt aaattcatca actggaactg ggcttccggt
     1081 ttgttcttcg gccagtagta aatcagcgta tcgcaatctg cgacgtcatc cgccttggcg
     1141 accagactaa aacgggcgtt atcccccatc tggcggctta atacctgcca gtggtggaat
     1201 tgctgggtat gagcacggct ggccgcggta tctaaacgcg cgggcaggtc atcctgtaag
     1261 tctccggcaa acagaatacg gctttgttcg aaatcatcac tgtgacgcag caagacttca
     1321 cttgccgggg taaatgcaga catggaatgc tcctcaattg atactggcgg cgattatagc
     1381 catatgttgg cgcggtatcg acgaatttgc tatatttgcg cccctgacaa caggagcgat
     1441 tcgctatgac atcccgacga gactggcagt tacagcaact gggcattacc cagtggtcgc
     1501 tgcgtcgccc tggcgcgttg cagggggaaa ttgccattgc gatcccggca cacgtccgtc
     1561 tggtgatggt ggcaaacgat cttcccgccc tgactgatcc tttagtgagc gatgttctgc
     1621 gcgcattaac cgtcagcccc gatcaagtgc tgcaactgac gccagaaaaa atcgcgatgc
     1681 tgccgcaagg cagtcgctgc aacagctggc ggttgggtac tgacgaaccg ctatcactgg
     1741 aaggcgctca ggtggcatca ccggcgctca ccgatttacg ggcaaaccca acggcacgcg
     1801 ccgcgttatg gcaacaaatt tgcacatatg aacacgattt cttccctcga aacgactgat
     1861 ttaccagcgg cttaccacat tgaacaacgc gcccacgcct ttccgtggag tgaaaaaacc
     1921 tttgccagca accagggcga gcgttatctc aactttcagt taacgcaaaa cggcaaaatg
     1981 gcggcgtttg cgattacgca agtggtgctg gatgaagcta cattgttcaa tattgcggtc
     2041 gatcctgact atcagcgtca gggattggga agggcgctgc tggaacatct gatcgacgaa
     2101 ctggaaaaac gcggcgtggc gacgctctgg ctggaagtcc gtgcctcaaa tgctgccgcc
     2161 attgccctgt acgaaagttt aggctttaac gaggcgacga ttcgccgcaa ttactacccc
     2221 accacggacg gtcgcgaaga cgccatcatc atggcgttgc caatcagtat gtaagacaag
     2281 gtggaataat gaagtgggac tggattttct ttgatgccga tgaaacgctg tttacctttg
     2341 actcattcac cggcctgcag cggatgtttc ttgattacag cgtcaccttt accgctgaag
     2401 attttcagga ctatcaggcc gttaacaagc cactgtgggt ggattatcaa aacggcgcga
     2461 tcacttcatt acagcttcag cacgggcgtt tcgagagctg ggccgaacgg ctgaaagttg
     2521 aagcaggctt gcttaacgat gcctttatta atgcgatggc ggaaatctgc acgccgctgc
     2581 cgggcgcggt ttctctgctt aacgccattc gtggcaacgc caaaatcggc atcatcacca
     2641 acggctttag cgccttgcag caagtgcgtc tggaacgcac gggcctgcgt gattacttcg
     2701 atttgctggt gatttccgaa gaagttggcg ttgctaaacc gaataagaaa attttcgatt
     2761 atgcgctgga acaggcgggc aatcctgacc gttcacgcgt gctgatggtt ggcgacactg
     2821 ccgagtccga tattctcggt ggcatcaacg ccgggcttgc gacctgctgg ctgaatgcgc
     2881 acaatcgcga gcaaccagaa ggcatcgcgc ccacctggac cgtttcttcg ttgcacgaac
     2941 tggagcagct cctgtgtaaa cactgattgc ctcccccccg ttgatgggta aaatagccgc
     3001 aatttttcgt tttcaacaag cgcggcgcga tgccgcttac tcaagaagaa agaattatga
     3061 cgttgtctcc ttatttgcaa gaggtggcta agcgccgcac ttttgccatt atttctcacc
     3121 cggacgccgg taagactacc atcaccgaga aggtgctgct gttcggacag gccattcaga
     3181 ccgccggtac agtaaaaggc cgtggttcca accagcacgc taagtcggac tggatggaga
     3241 tggaaaagca gcgtgggatc tccattacta cgtctgtgat gcagtttccg tatcacgatt
     3301 gcctggttaa cctgctagac accccggggc acgaagactt ctcggaagat acctatcgta
     3361 ccctgacggc ggtggactgc tgcctgatgg ttatcgacgc cgcaaaaggt gttgaagatc
     3421 gtacccgtaa gctgatggaa gttacccgtc tgcgcgacac gccgatcctc acctttatga
     3481 acaaacttga ccgtgatatc cgcgacccga tggaactgct cgatgaagtt gagaacgagc
     3541 tgaaaatcgg ctgcgcaccg atcacctggc cgattggctg cggcaagctg tttaaaggcg
     3601 tttaccacct ttataaagac gaaacctatc tctatcagag cggtaaaggt cacaccattc
     3661 aggaagtccg cattgttaaa gggctgaata acccggatct cgatgctgcg gttggtgaag
     3721 atctggcgca gcagctgcgt gacgaactgg aactggtgaa aggcgcgtct aacgagttcg
     3781 acaaagaatt gttccttgcg ggcgaaatca ctccggtatt cttcggtact gcgctgggta
     3841 acttcggtgt cgatcatatg ctggatggcc tggtggagtg ggctcctgcg ccgatgccgc
     3901 gtcagactga tacccgtacc gtagaagcga gcgaagacaa atttaccggc ttcgtattta
     3961 aaattcaggc caacatggac ccgaaacacc gcgaccgcgt ggcgtttatg cgcgtggtat
     4021 ccggtaaata tgaaaaaggc atgaagctgc gccaggtgcg caccgcgaaa gacgtggtga
     4081 tctctgacgc gctgaccttt atggcgggtg accgttcgca cgttgaagaa gcgtatcctg
     4141 gcgatatcct cggcctgcac aaccacggca ccattcagat cggcgacacc tttacccagg
     4201 gtgagatgat gaagttcacc ggtattccga acttcgcacc agaactgttc cgtcgtatcc
     4261 gcctgaaaga tccgctgaag caaaaacagc tgctcaaagg gctggtacag ctttccgaag
     4321 agggcgcggt gcaggtgttc cgtccgatct ccaacaacga tctgatcgtt ggtgcagttg
     4381 gtgtgctgca gtttgatgta gtggtatcgc gcctgaagag cgaatacaac gttgaagcag
     4441 tatatgaatc agtcaacgtt gccaccgccc gctgggtaga atgtgcagac gcgaagaaat
     4501 tcgaagagtt caagcgtaag aacgaaagcc aactggcgct tgatggcggc gataacctcg
     4561 cttacatcgc taccagcatg gtcaacctgc gcctggcgca ggaacgttat ccggacgttc
     4621 agttccacca gacccgcgag cattaattct tgcctccagg gcgcggtagc cgctgcgccc
     4681 tgtcaatttc ccttccttat tagccgctta cggaatgttc ttaaaacatt cacttttgct
     4741 tatgttttcg ctgatatccc gagcggtttc aaaattgtga tctatattta acaaagtaat
     4801 gacatttctg acggcgttaa ataccgttca atgcgtagat atcagtatct aaagccgtcg
     4861 attgtcattc taccgatatt aataactgat tcagaggctg taatggtcgt tattcatcac
     4921 ccatcgcttt tgtgatggcg accattgact tctgtagagg gtgaagtctc tccctattca
     4981 gcaatgcaac ctcgtgttgc caggctcaaa ttacgagcaa acatacagga ataaatcgat
     5041 gactatgaca agactgaaga tttcgaaaac tctgctggct gtaatgttga cctctgccgt
     5101 cgcgaccggc tctgcctacg cggaaaacaa cgcacagact accaatgaaa gcgcagggca
     5161 aaaagtcgat agctctatga ataaagtcgg taatttcatg gatgacagcg ccatcaccgc
     5221 gaaagtgaag gcggcgctgg tggatcatga caacatcaag agcaccgata tctctgtcaa
     5281 aaccgatcaa aaagtcgtga ccctgagcgg tttcgttgaa agccaggctc aggccgaaga
     5341 ggcagtgaaa gtggcgaaag gcgttgaagg ggtgacctct gtcagcgaca aactgcacgt
     5401 tcgcgacgct aaagaaggct ctgtgaaggg ctacgcgggt gacaccgcca ccaccagtga
     5461 aatcaaagcc aaactgctgg cggacgatat cgtcccttcc cgtcatgtga aagttgaaac
     5521 caccgacggc gtggttcagc tctccggtac cgtcgattcc caggcacaga gcgaccgtgc
     5581 tgaaagtatc gccaaagcgg tagatggtgt gaaaagcgtt aaaaatgatc tgaaaactaa
     5641 gtaattcgtc gtaattcgtc ctcccgaact ttgtcgggag gcgcgatgtg caccacacta
     5701 aaaatatcgc attgagtgat tttcacgctc atatcaagcg gtgacattaa ctatggtaaa
     5761 ggagacgctt atgtttcgtt ggggcatcat atttctggtt atcgcgttaa tcgccgccgc
     5821 acttgggttt ggtggtctgg ccggtaccgc tgcaggcgca gctaaaattg tctttgtcgt
     5881 cgggattatt ctgttcctgg tgagtttgtt catgggccga aaacgaccct agatttcaga
     5941 ccattaagaa aattttatcc aaagccagtc cagcggactg gcttttgcgg ttttagcgaa
     6001 taataaattg cgttactttg tcattctttc acaacaaaaa caggaaggca gaggtggggc
     6061 agcgaatacc tgtaacgctt ggtaatattg cgccgttgtc gctaaggccg ttccagcctg
     6121 gacgaatagc tctggtgtgc gaaggcggcg gacagcgtgg aattttcacg gctggcgtgc
     6181 tggatgagtt tatgcgcgcg cagtttaatc ctttcgatct ttatctcggc acatctgccg
     6241 gggcgcagaa cctctcggcg tttatctgca atcagcccgg ttacgcgcgc aaagtcatca
     6301 tgcgctatac cacaaaacgc gaatttttcg atccattgcg ctttgtccgt ggaggaaatc
     6361 ttatcgatct cgactggctg gtggaggcca ccgcaagcca gatgccgttg caaatggata
     6421 ccgccgcgcg gttgtttgac agcggcaaat cgttttatat gtgcgcctgt cgtcaggatg
     6481 actacgcgcc taattacttt ttaccaacca aacaaaactg gctggatgtg attcgcgcct
     6541 ccagtgcgat acctggcttt tatcgtagcg gagtgtcgct ggaaggcatt aactacctgg
     6601 atggcgggat cagtgatgcg attccggtta aagaggcggc aaggcagggc gctaaaacgt
     6661 tggtcgtcat ccgcactgtg ccgtcacaaa tgtactacac gccgcagtgg ttcaaacgca
     6721 tggaacgctg gctgggtgac agtagcctgc agccgctggt caatctggtg cagcatcatg
     6781 aaaccagcta tcgtgacatt cagcaattta ttgagaaacc accgggcaag ctgcggatat
     6841 tcgaaattta tccaccgaaa ccattacata gtatcgcgct tggcagtcgg attccggcgc
     6901 tgcgtgaaga ctataaactt gggcgtttat gcggtcgtta tttcctcgcc acggttggca
     6961 agctattaac tgaaaaagcg ccgcttaccc gccatctggt gccagtggtg acgccggaat
     7021 cgatcgttat tccgcctgcg ccagtcgcca acgatacgct ggttgccgaa gtgagcgatg
     7081 ctccgcaggc gaacgacccg acatttaaca atgaggatct ggcttgattt gccgttttat
     7141 cgatacccac tgccattttg atttcccgcc gtttagtggc gatgaagagg ccagcctgca
     7201 acgcgcggca caagcgggcg taggcaagat cattgttccg gcgacagagg cggaaaattt
     7261 tgcccgcgtg caggcgttag cggaaaaata tcaaccgctg tatgccgcat tggggttgca
     7321 tccggggatg ttggaaaaac atagcgatgt gtctcttgat cagctacagc aggcgctgga
     7381 aaggcgtccg gcgaaggtgg tggcggtggg ggagatcggt ctggatctct tcggcgacga
     7441 tccgcaattt gagaggcagc agtggttact cgacgaacag ctgaaactgg cgaaacgcta
     7501 cgatctgccg gtgatccttc attcccggcg tacacacgac aaactggcga tgcatcttaa
     7561 acgtcacgat ttaccgtgca ctggcgtggt tcacggtttt tccggcagcc tgcaacaggc
     7621 cgagcggttt gtacagctgg gctacaaaat cggcgtaggc ggtactatca cctatccacg
     7681 cgccagtaaa acccgcgatg tcatcgcaaa attaccgctg gcatcgttgt tgctggaaac
     7741 cgacgcgccg gatatgccgc tcaacggctt tcaggggcag cctaaccgac cggaacaggc
     7801 tgtgcgggtg tttgacgtgc tttgcgagtt gcgcccggaa ccagaagatg agattgccga
     7861 agtgttgctt aataatacgt atgcgttgtt tagcgttagt gggtagttta cccttcgtag
     7921 gcagcatccg gcagtcgg
//
LOCUS       DAZKGS010000188         7902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKGS010000188 DAZKGS010000000
VERSION     DAZKGS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..1571
                     /locus_tag="ACHOQY_004228"
     CDS             300..1571
                     /locus_tag="ACHOQY_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9680204.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHOQY_004229"
     CDS             complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHOQY_004229"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9680205.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHOQY_004230"
     CDS             complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHOQY_004230"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9680206.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHOQY_004231"
     CDS             complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHOQY_004231"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9680207.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHOQY_004232"
     CDS             complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHOQY_004232"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9680208.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHOQY_004233"
     CDS             complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHOQY_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9680209.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7637..>7902)
                     /locus_tag="ACHOQY_004234"
     CDS             complement(7637..>7902)
                     /locus_tag="ACHOQY_004234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680210.1"
                     /translation="GTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVTETIVFF
                     YICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1818 a         2138 c         2024 g         1922 t
ORIGIN      
        1 ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
       61 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
      121 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
      181 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
      241 gcaagagaaa ctgggtttat tcatttttgc gagaccgact tctttctgga caggacttta
      301 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
      361 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
      421 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
      481 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
      541 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
      601 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
      661 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
      721 aagtcgctat tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta
      781 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
      841 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
      901 gcgatgtcct gcttacaatt ccattttgct tcttttctgc ggtatttatt caggtactta
      961 acccaatgaa tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg
     1021 cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
     1081 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
     1141 cggcactggc gctggccgcg caggtgatcc ctggacatat cattcatatc acctctacgg
     1201 tgctgaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
     1261 ctattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
     1321 cacgcatgct gactctggcg atctgcgctt ttatcgtcat tacattgacg atttgggttt
     1381 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt
     1441 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
     1501 ttaaagcctg gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc
     1561 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
     1621 cctgatcaac agcaaaacaa gcgaccagtt gaccgccgta gtcttttagc tgcggctgca
     1681 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag
     1741 gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt
     1801 tcaggcgtgg cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga
     1861 tttggtcctt cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag
     1921 caatgtgctc caccaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct
     1981 gctgcaaatc catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa
     2041 ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac
     2101 gctgacgttg tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
     2161 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt
     2221 taataagcag cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt
     2281 aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
     2341 gcttgagcag atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg
     2401 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
     2461 tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga
     2521 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
     2581 gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
     2641 gcatttggac tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct
     2701 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc
     2761 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
     2821 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc
     2881 ggtttccacc acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag
     2941 cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag
     3001 tagttcgatg atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat
     3061 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
     3121 ggaaagctga tgcgggtaaa tatccagacg cgatgccgga tcgggaatac cgacctgatt
     3181 cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat
     3241 cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
     3301 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg
     3361 caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag
     3421 ccccataatc gccagtgaac tgaccgactt accggaacca gactcaccca caatcccgac
     3481 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc
     3541 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
     3601 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
     3661 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
     3721 gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga
     3781 ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
     3841 tcagcggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
     3901 ccgccacgcg agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg
     3961 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
     4021 acgggccgaa gattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga
     4081 tatcgaccac gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc
     4141 cgagaataac gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca
     4201 ggcgcgcacc gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca
     4261 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact
     4321 gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga
     4381 cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
     4441 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
     4501 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
     4561 gcaagttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
     4621 agtcgcggcg ttgcagcgcg tcaatcaacc agcgccccag accgggccac gagaagatgg
     4681 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta
     4741 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt
     4801 tggcgcgcgc ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca
     4861 tacgcacaat gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg
     4921 cgacggcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac
     4981 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
     5041 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
     5101 tcgagtagcc ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa
     5161 ccgcagccag cacgccgacc ggaataccga ccgcagtcgc aaaaatcatc gcgcagacgc
     5221 caagttccag cgtggcctgg aggcgcggca cgaactcttc ccaaaccggg atgcggcttt
     5281 tcattgaaat gcctagatcg ccatgcataa cgccccaaat gtaatggaga tactgctgcc
     5341 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctct ggggagatcc
     5401 cacgttcgcc cgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
     5461 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
     5521 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
     5581 cacaaatcaa ttcccgccta ggtcgggctg ggaatgaagt attgctgccg gatggcgctg
     5641 cacgcagagg cacagcgcca ccgggtatgg cttttaatta ttcgatagag acgttttcga
     5701 agtgatgttt gcctaatgga tcaaccacat agcctttaac ttctttgcgt accggttcaa
     5761 acacggtgga gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc acctgcgctt
     5821 gcttgtacag ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt tgaatcagat
     5881 cttcaaacgg tttgtagcac cattttgagt agttggagcc ttgttcagag gcggcgcagc
     5941 tgaacagggt ggcgaagaag ttatccggat ccccgttatc gccagtccag cccatcatta
     6001 ccgtctggtg ctcgccatct ttcgcacgct tgaggtactc accccattcg taggtgacaa
     6061 ttttggcctg cacgccgact ttcgcccagt cagcctgaat catctccgcc atgcggcgag
     6121 cgttcgggtt atatgggcgc tgtaccggca tcgcccacag gtcgatggag aaaccttttt
     6181 ccagacccgc ttctttcagc aaggctttcg ctttttcagg atcgtaggtg taatcctgaa
     6241 cgtcgtcgtt atagccccac atggttggcg ggatcaggtt tttcgctgat acacccgcgc
     6301 cctgataaac cgctttgatg atcgcgtctt tgttcaccgc gtaggtcagt gcctggcgaa
     6361 ctttcacgtc atccagcggt tttttctgca cgttatagga gaggtagccg acgttcagcc
     6421 ccggcatttc catcaggttg atggatttat cctgcttcat gcgagcgatg tctgccgggt
     6481 tcgggtacgg catcacctgg cattcattct tctgcaattt cgcataacgc acggaagcgt
     6541 caggagtaat agagaagacc agcgtatcga tctgcggttt ggtgccccag tagccatcaa
     6601 acgctttgta gcggatacgg gagtcttttt ggtactgctg taactggaac ggaccggtgc
     6661 cgattgggtt gaggtccagt ttttccggtg taccggcttt catcatcgcg tcagcatatt
     6721 cttttgacag aatagaggcg aagtccattg ccaggtcagc gaggaacggc gcttccgggc
     6781 gagtcagcac aaactgaacg gtgttgtcgt ccaccttttt cacttcgctg atcagctccg
     6841 gcaagcccat gccttcgaag tattcgtagc tgccgccaga aactttatgg tacgggtttt
     6901 gcgcgttttt ctgacgatca aacgagaaca ccacgtcatc ggcgttcagt tcacgcgtcg
     6961 gtttgaattc tttattgtcg tgccacttca cacctttacg cagatggaag gtataggttt
     7021 taccgtcttc gctgacttcc cacttttcag cgaggcccgg gatcacttca gtggtgccga
     7081 ttttaaattc aaccaggcgg ttataaagcg gtacggaaga ggcgtcatag gtggtgccgg
     7141 aggtaaacag ctgcgggtta aacccttccg gagatccttc tgagcaataa accagagttt
     7201 tagcctgaac acttgctgcg acggtcatag ccaccaggct gagaccaagc ttcagcatcc
     7261 ctgacttttt caaggaaata cgcattattc tgctccaatt gtgatgtttg ttgttttaac
     7321 cctttgcagt gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc gggtcaacgt
     7381 tatgaggtgg ggatgccgta ctaattaaca tcagtgtaac agtgcggatc ctccataaaa
     7441 tgcccctcgt gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca gccaaccggg
     7501 atttacacca acggtgagaa tccacaaaca aagattaaaa aaacttcaaa cagctatttg
     7561 cagcagagaa atttgtgcta ctccaacatg accagaacaa tcagcttaat atttagcaac
     7621 atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg
     7681 agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt
     7741 aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt
     7801 taatggatag tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga
     7861 ataaggcggt cgcgccgcat ccgacaataa gtgcggtgcc gt
//
LOCUS       DAZKGS010000189         7900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKGS010000189 DAZKGS010000000
VERSION     DAZKGS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1080
                     /locus_tag="ACHOQY_004235"
                     /pseudo
     CDS             1..1080
                     /locus_tag="ACHOQY_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1049..2785)
                     /gene="aceK"
                     /locus_tag="ACHOQY_004236"
     CDS             complement(1049..2785)
                     /gene="aceK"
                     /locus_tag="ACHOQY_004236"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9680211.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3066..4370)
                     /gene="aceA"
                     /locus_tag="ACHOQY_004237"
     CDS             complement(3066..4370)
                     /gene="aceA"
                     /locus_tag="ACHOQY_004237"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9680212.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4400..6001)
                     /gene="aceB"
                     /locus_tag="ACHOQY_004238"
     CDS             complement(4400..6001)
                     /gene="aceB"
                     /locus_tag="ACHOQY_004238"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9680213.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6270..7199)
                     /gene="metA"
                     /locus_tag="ACHOQY_004239"
     CDS             complement(6270..7199)
                     /gene="metA"
                     /locus_tag="ACHOQY_004239"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9680214.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7356..7799
                     /locus_tag="ACHOQY_004240"
     CDS             7356..7799
                     /locus_tag="ACHOQY_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9680215.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1977 a         1978 c         1988 g         1957 t
ORIGIN      
        1 atttatcaga atgttgatta caaaccccat caagatgatt actcttcagc tgaaaaattt
       61 ctcactcact tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct
      121 gaggggcgtt gtgttgctgc ctgtggtgat ttcgggctag ttatgaaggc atatttcgac
      181 aagatggaat caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct
      241 ttgacggtcc ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg
      301 gtttatgatc ctaatgtcac taacgataaa ataagaatta tgagcgaaag caaagaggat
      361 attaaacact attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca
      421 aatgatcata ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta
      481 ttgatgttaa aaggatctgt ggatgaaata acccctccat tatcaccagc aacgatgaat
      541 ttgctaatgg caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa
      601 atgccagaac tacatagaac agaaatgttg actgcctata atagtggaca tatgaacgtt
      661 attaatacta tttttaacgc ataacccact ctgtttaata cgtttaaatt cgataaaaaa
      721 aatatgaagc ccctcctcct ggcaaataat tctaatgaat atcccggttt gttttcagcg
      781 atacagcata aacaacaaaa tgttgtagag acggtttatc ttgctttatc taaccatgca
      841 cgcctgtttg gatttaccgc tgaagatatt atggattttt ggcaacacaa agccccacaa
      901 aaatactctg cctttgagtt ggcttttgaa ttgggtcacc gggttattgc tgaattaatc
      961 cttaatacat taaataagat ggctgaaagc tttggcttta cggataaccc tcgatacatt
     1021 gcggagaaaa attatatgga agctttactc aaaaaagcat ctccccatac cgtacgctaa
     1081 atctttgcct gcgccgatac gcataaacat cttccacatg cccttcacgt atgcggtttt
     1141 gtagtgcgcg ccagtaatca gcgcggaaca ggtcggcgtg catctcttca aacagcggac
     1201 caatacgcgg gtcggcgcat agccagtggc gaaactcttc cgggaaaaca tcgcccggcg
     1261 agacgctgta ccacggttcg ctggcaagtt cgtcttccgg atagcgcggc ggcgggatgt
     1321 cgcggaaatt cacttccgtc atgtagcaaa tttcatcgta atcataaaaa accacacgcc
     1381 cgtgacgggt gacaccgaag tttttaaaca gcatgtcgcc aggaaaaatg ttagcagcgg
     1441 caagctggcg aatagcgtta ccgtattctt caatggcgtc gcgcaactgc tgaccttcca
     1501 cttgttccag ccagatattg agcggcacca tccgccgctc gatatagaga tggcgaatta
     1561 caatctgctc accaagatcg gtgatttttt ccgctgcttc ctggagcagt aattccatta
     1621 atgccgggga aatatgccgc ttctccagca caaagttttc aaactcctgg gtgtccgcca
     1681 ttcggcccac acggtcgtgc tctttcacca gttgatagca ggcacgaacg tgagcggcag
     1741 acatctcttt ctgcggcgcg aacttgtctt tgatgacttt gaatacccga tcaaagcccg
     1801 gcagcgtaaa taccaacatc accattccac gaatacccgg cgcttcaatg aactgctcat
     1861 tacagccctg tagataaacg agatattcgc ggtagctttc ggttttggcg tgcttctggc
     1921 agccgatagc catatacaat tcagcagtgg ttttacccgg cagaatttcc cgtagccact
     1981 cgaccagcgc tgcgggcagc ggtgcataaa ccataaaata agaacgcgca aagccaaaaa
     2041 caatgctcgc ttcggcggtc gtcgtcaggc aggtatcaat aaataactcg ccgtcgtccg
     2101 tctggtggat cggcagtaag aagggcaacg tgccggaagg cgtgatcagt ttgcccacca
     2161 gccaagcggc tttattgcgg taaaacagtt cgttcgccac ctgtaaatga ctttccgcga
     2221 ggttgtctgt ccccagcgtt tccgtcagat ggcgaatgat gtaatgaatg tcacggcttt
     2281 tattctgcca gcgcaggcgc agcggcaggt cgctgataac gcgcatcagt agagattccc
     2341 agccgtgatc ggggtgaaag tctttcgcca gcgggcgggg aatggtacga aagcggcgct
     2401 ctggctgaga gctaaagata aaaagccgct cgggagtaag cgagcggtgg tcaaataacc
     2461 gacagtacac ggagttaaaa aagctctccg caatctcgaa gtgcgggtaa tccggcaaca
     2521 gccgcgtgta atgctcttta acgcgcagta aaaatgccgc gtccgtactt tggccgttag
     2581 taatgcagcg cagttgctcc acgaccagac caacgtgatg atcgtaaaga tggatacggt
     2641 ttttcatcgc ctgctggaca gcatgccagt cggcctgttc gaaacgctgc tgcgccccgg
     2701 aagtcacttc gaggaatcga ccatactgag cgtcgaagcc ttgcaaaatg gtttgagcaa
     2761 tcagtaattc caggccacgc ggcatttagc gccctcatca ggagcagaga attgccggat
     2821 gcggcgtaaa cgccttatcc agcctacgtt cggcaacggt tgtaggcatg ataagacgcg
     2881 caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc ggcgtaaatg ccttatccgg
     2941 cctacgttcg gcaacggctg taggcatgat aagacgcgca agcgtcgcat caggcatcgt
     3001 gcaccgattg ccggatgcgg cgtgaacgcc ttatccggcc tacagtcagc aacggttgtt
     3061 gttgcttaga actgcgattc ttcagtggag ccggtcagcg cggtgactga agacgtgccg
     3121 ccctgaataa tagtcgtcac tttatcgaag taacctgtac ccacttcctg ctggtgagat
     3181 acgaaggtat agccatcttt cgcggcggca aattccggct gctgcacttt ctcaacgtag
     3241 tgcttcatac cctcgccctg ggcataggcg tttgccaggt caaacatgtt gaaccacatg
     3301 ctgtggatac ctgccagggt gatgaactgg aacttgtagc ccatatccga cagctgctgc
     3361 tggaagctgg caatagtttt gtcgtcgagg tttttctgcc agttgaacga cggcgagcag
     3421 ttataagcca gcagtttgcc cggatatttc gcgtggatag cttgtgcaaa gcgacgcgcc
     3481 agttccagat ccggcgtgga ggtttcacac cagaccaggt cagcatatgg cgcatacgcc
     3541 aggccacggc tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc ttcactggta
     3601 cgctcgccag taataaattc actgtcatac gggtcgcaat cggaggtaat cagatccgcc
     3661 gcatcggcat cggtacgagc aaccagcagg gttggaacgc ccgtcacgtc agctgcaaga
     3721 cgcgccgcga ccagtttctg aatagcttcc tgagttggca ccaaaacttt gccgcccatg
     3781 tgaccgcatt tcttcactga cgccagctga tcttcgaagt gaactgccgc tgcaccggct
     3841 tcaatcatcg ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca
     3901 tcggcaacga tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcctgcg
     3961 gaccattgga tctgatcggc acgacggaag gtgttgttga tccgctccac cacagctggc
     4021 accgagtttg ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc
     4081 gccgctacct gccatcccga cagatagact gcttcaatac ccgctttcgc ctgttgcagc
     4141 gcctgaccgc cagtcagtgc accgaggctg ttgatgtagc cttttttcga ctcgccatgc
     4201 agcagacgcc acattttcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa
     4261 ccgcgtaatt tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt
     4321 tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc
     4381 tccatagtta tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc aggaaatcaa
     4441 ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca aaacgcccct
     4501 gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttca cccagcatct
     4561 ggcggaacaa ggctttggtc accggtttgc cattgctcaa cgttttttga tgatggatcc
     4621 actgccagat tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa
     4681 tcggcacgca gccgttgccg gagatccatg cttcgatgta ctgcactgcc acgcgaatgt
     4741 tggcgcgcat accttcttcg gtgcgttcac catcacaagg tgccagcagc tgatcggcag
     4801 taatcggcgc gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt
     4861 cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg agcaatccat gtgccatcgt
     4921 gaccgttatt ggcttccagc gctttatccg cttttacttt gttgagcacc tggttattgc
     4981 gctcttcatc tttgctcgga ataaacgctg ccatgccgcc catcgcaaaa gcaccgcgtt
     5041 tatggcaggt tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca
     5101 ctgcctgtct gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga
     5161 agatgtaatc ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga
     5221 tttcatccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc
     5281 cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt
     5341 cctgccagga ctgggttttc ggcagataga aatagggacc gctgcccttt gccaacagtg
     5401 cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg attgcctcac
     5461 cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg
     5521 ctggattggg cttgagctga taaattttgc ctgcttcatt ggtatagctg atggtgccgt
     5581 taaccgcatc gcgcaggtta atttgcccgt cgatcacttt gttccagtct ggtgccagtg
     5641 aatcttcgaa atcggccata aagactttca cattggcgtt cagtgcgttg atcaccatct
     5701 tgcgctcaac cgggccagtt atctcgactc gacgatcttg taagtccgcg ggaataccac
     5761 gaattttcca gtccgtatcg cgaatggaag ctgtttccga aataaaatca ggcaaagttc
     5821 cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattg cgttgtggcg
     5881 taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct
     5941 cctgctcgcc atacggcctt atgaaagcca gttcatcggt tgttgttgcc tgttcagtca
     6001 tcgtgcagct cctcgtcatg gacccgaaaa cttccccact gtgaacgaag gatcgttgtg
     6061 cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc
     6121 cattttaatt aaaaatactt gataacttat tgataacaat atgaataaaa tttattcacc
     6181 tcctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga
     6241 atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga
     6301 tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg
     6361 tgactacgcc agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac
     6421 ggtacttccg ggcctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc
     6481 gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga
     6541 tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac
     6601 gctgccggaa agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc
     6661 gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg
     6721 cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca
     6781 aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag
     6841 taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca
     6901 aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc
     6961 gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag
     7021 tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg
     7081 attagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag
     7141 acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aataggcata
     7201 acctgattac ctcactacat acgcttatac gtttagacat ccagatagct gaagataacc
     7261 agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaaggct
     7321 acgttagaat ataagaatga cgaaaaggag agaaaatggt tattagtatt cgccgctcac
     7381 agcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacccacg
     7441 attttttatc agcagagtat cgggccgagc tggaagagct ggttcgttcc tttctgccgg
     7501 aagcaccgtt gtgggtcgcg gttaatgaac gggatcagcc ggttgggttt atgttgttaa
     7561 gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gcgcggctgc ggcgtgggtc
     7621 ggatgctggt ggagcatgcg ctctcgatgg caccggaact gacaaccaac gttaatgagc
     7681 aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg ggacgctctg
     7741 aggtggacga tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg ggggaataag
     7801 ccgcccctgc cgtgcctgcc caggctggag aagacgcgcc agcgtcgcac ccggcatttt
     7861 tttcaccaac gcaaaaaggc catcgtgact gatggccttt
//
LOCUS       DAZKGS010000190         7853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKGS010000190 DAZKGS010000000
VERSION     DAZKGS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..1204)
                     /gene="ugd"
                     /locus_tag="ACHOQY_004241"
     CDS             complement(38..1204)
                     /gene="ugd"
                     /locus_tag="ACHOQY_004241"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP9680216.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(1454..2860)
                     /gene="gndA"
                     /locus_tag="ACHOQY_004242"
     CDS             complement(1454..2860)
                     /gene="gndA"
                     /locus_tag="ACHOQY_004242"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9680217.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(2971..3378)
                     /locus_tag="ACHOQY_004243"
     CDS             complement(2971..3378)
                     /locus_tag="ACHOQY_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFP9680218.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(3369..4577)
                     /locus_tag="ACHOQY_004244"
     CDS             complement(3369..4577)
                     /locus_tag="ACHOQY_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9680219.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(4577..5707)
                     /gene="wecB"
                     /locus_tag="ACHOQY_004245"
     CDS             complement(4577..5707)
                     /gene="wecB"
                     /locus_tag="ACHOQY_004245"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9680220.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(5704..6810)
                     /locus_tag="ACHOQY_004246"
     CDS             complement(5704..6810)
                     /locus_tag="ACHOQY_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFP9680221.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(6812..7846)
                     /gene="fnlA"
                     /locus_tag="ACHOQY_004247"
     CDS             complement(6812..7846)
                     /gene="fnlA"
                     /locus_tag="ACHOQY_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HFP9680222.1"
                     /translation="MFKNKTLVITGGTGSFGNAVLKRFLDTDITEIRIFSRDEKKQDD
                     MRKKYNNSKLKFYIGDVRDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVL
                     GTENVLEAAIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRAL
                     RSGEDYELDS"
BASE COUNT         2306 a         1754 c         1535 g         2258 t
ORIGIN      
        1 agagaatcag ggcgaaaatc gccctgataa caggatgtta gtcactgcca aagagatcgc
       61 gggtgtagac tttgtctgcc acatccttaa gctcttctgc catacggttg gagataatta
      121 cgtcggcttg ttgtttgaaa gtggcgagat cacgttccag ccgagagttg aagaatgagt
      181 cttctttcat cactggctcg tagatgatta cttcaacacc tttcgccttg atacgcttca
      241 taatcccctg aatagaagac gcacggaagt tatctgaacc gctcttcata atcaggcgat
      301 aaatacccac cacatgcggt ttgcgtgaca aaatggcatc ggcaataaaa tctttacgcg
      361 tgcggtttgc atcgacaatt gccgagatca ggttattcgg cacagactga tagttcgcca
      421 gtaactgctt ggtatctttc ggcagacaat aaccaccata accaaacgac ggattgttgt
      481 aatggttgcc aatacgcgga tcgagacaaa cgccttcgat aatctggcga gtattcagac
      541 ctaaactttc tgcgtagcta tccagttcat taaagtacgc tacgcgcatc gccagatagg
      601 tattagcgaa aagtttaatc gcttctgctt cggttgagtc ggtaaacagg gtcgggatat
      661 tttgcttaat cgcgccttcc tgtaacagag cagcaaaacg ttctgcgcgt tctgaacgct
      721 caccgatgac aatacgtgaa ggatggagat tatcgtaaag ggctttaccc tcacggagaa
      781 attccggaga gaaaataata tttttagtac gatatttctt atgcatcgct gcggtaaagc
      841 caacgggtac cgttgatttg atgaccataa ccgcataagg atttatctct actacgtcct
      901 taattactga ttctacactc gatgtattga aataattagt gttaggatca tagtcggttg
      961 gagtggcgat gatgacataa tcagcatccc ggtaggcttc atttttatct aatgtggcat
     1021 taaagtgtat tttatctgat tgcaaaaact gctgaatttc cttatcaaca ataggagata
     1081 tccgatcatt cagcatagca acgcgtgacg gtaaaatatc taatgccaca acctcatgat
     1141 tttgtgcgat tagaagcccg tttgacaagc ctacatagcc ggtaccggaa atggtgattt
     1201 tcatttcatg ctctcagaat taacttaact gtgaatcatg atgtttttag catcctgata
     1261 agagctaaaa gttttaacgc tacgttgttc gatggtcaac gcaacgaaaa acaattcaga
     1321 ttttctttat aagaatagct gatatttatt aagttaatat taagcagtga aatttagtct
     1381 ataagataat tggcaaaaaa aaaagcccgg cgtaatatac gccgggcctc aatttattgt
     1441 tggttaaatc agattaatcc agccattcag tatggaacac accttcttta tcaatgcgct
     1501 tataagtatg cgcaccaaaa tagtcacgct gagcctggat caggttcgca ggcagaacag
     1561 cggcacggta gctgtcataa taggcaaccg cagcggcgaa ggtcggaacc gggataccgt
     1621 tctgtactgc ataagcaacg acatcacgca gcgcctgctg gtagtcatcg gcaatttgct
     1681 tgaagtacgg agccaacagc aggttagcga tctgaggatt ttcggcataa gcatcagtta
     1741 ttttctgcag gaactgcgca cggatgatgc agccagcacg gaaaatcttc gcgatttcgc
     1801 cgtagttcag atcccagttg tactcttcag acgctgcgcg cagctgagag aagccctgag
     1861 cgtaagaaac gattttgccc agatacagcg cacggcgaac tttttcgatg aactcagcct
     1921 tgtcgccagc tggctgcgct tgcgggccag agagaacttt agatgcggca acacgctgat
     1981 ctttcagaga agagatataa cgtgcaaaca cagactcggt aatcagcgac agcggttcac
     2041 cgagatccag cgcgctctgg ctggtccatt taccggtacc tttgttagcc gcttcatcca
     2101 ggatcacatc aaccaggtag ttaccgtctt catctttttt ggtgaagatg tctttggtga
     2161 tgtcgatcag gtagctactc agttcaccgt tattccactc ggtaaaggtc tgtgccagtt
     2221 cttcgttaga gagattcagg ccgcctttca ggagagaata ggcttcagca atcagttgca
     2281 tatcaccgta ttcaataccg ttgtgaacca tcttcacata gtgacccgcg ccatcagcac
     2341 caatataggt aacgcacggt tcgccatctt cagcaacggc ggcgatttta gtcaggatcg
     2401 gtgcaaccag ttcataggct tctttctgcc caccaggcat aatggaagga cctttcagtg
     2461 cgccttcttc accaccggag acaccggtac caatgaagtt aaaaccttct gcagaaagct
     2521 cacgattacg acgaatggtg tcctggaaga aggtattacc accatcaatg atgatgtcac
     2581 ctttatcgag gtatggctta agggaatcaa tagcagcatc cgtgcctgca cctgctttca
     2641 ccattaacag gatgcgacga ggcgtttcca gagattcaac aaactctttc accgtatagt
     2701 aaggaaccag tttcttgcct ggattttcgg caatcacttc ttccgtcttt tcacgggaac
     2761 ggttgaaaat agagacggta taaccacggc tttcgatgtt gagcgcaagg ttgcgcccca
     2821 tcactgccat accgactacg ccgatctgtt gctttgacat tgtttactcc tgtcaggggt
     2881 ggtcacgcgg ggttacgcgg cttgaagaag ctaagatggt aacccagcat gtatagaata
     2941 agatagaaga ataaacggtg gtgtatcgcc ctataaccac ttagaaaaag ccttagcttt
     3001 cagtgggtca aatggcccct cctttatctc taacataagg gcttttggtg acaggcattt
     3061 gacactatgt atatctcctg gggaaaattc cacgacactt attcccgtac cgtctccaac
     3121 aacaaacttt ttttggagct caccattttg ctcatacaaa caaacttcta attgcccttg
     3181 catgacgaca aacatttccc actgatgcgg taactcatgc caatggggtt caacatagct
     3241 gtcgcgtaca aatgcaataa gtaaacgttg aaccttctcc tgatgagatg cgtgcaataa
     3301 ataatgagcg cgcaaacgtt cagaatttgc tgcagtgcga aaaagttcat ccataatatt
     3361 gtcatcaact aaacgcattc tcctgcctcc agtcggactt cgatagtatg tgccgccgat
     3421 tcaaccgaaa attgagactt taacaacaca ttggcgttct gacctaactg ttttcttaaa
     3481 actgaatcac taagaagcaa ttgtgcagat tcaaacagtt tatcatcttc accattaaca
     3541 tgaatgaacc cagccctgtg cttattaatt acatccatta gatcattacc gccattcaca
     3601 ctcccaagga tcgggattga ttgaaccata taccctagta attttccggg gaaattatgt
     3661 gaagaatggc gagacgaaag ggagaacagg ccaacatcaa cttcagataa aattaattta
     3721 aactcttcct ggttcactga aggtagatga gtgaaattag ttaaattcca ttctgcagaa
     3781 agagatttaa tcaggtcaac ttcatcaccc tgccctataa acaggaaatg agcatcatga
     3841 tgacgcatca tattacgcgc aaggcgcatt aagtttgcca tatcctgagc atgcccaata
     3901 ttaccgccat agaaaaaaat gattttatct tttagagcgt atttttgacg aagtgaatga
     3961 taatcatcgc cggcagacac aggagtcatt gaggcccaat tacgtaaaac ttcacacgga
     4021 taacctttat tggcctgacg aaatatctca agattcttat cagacattaa cccaatccag
     4081 tcagcctgtt gatatgattt tttttcaaaa tacctgaaat atttttcaat tggcgagccg
     4141 gcttttatca taccagcatc aatgacccac tgtgggaaca tatcccttag gaccagatag
     4201 cttgggcact ggcatcgctc tttaattttt ttaaccaagc ctccccaaaa aatagagggg
     4261 gaataataaa cgataccatc aaatgtatca tgttggatga gatgctttaa tgcgtgccat
     4321 gcacgaaaag ataaaagagt ttcatttatg gcacgtttag ccttacctat atcctttaaa
     4381 ggtccacttt tgaaacgcca aacctttata ccatcagtca tgctaacaga atagattgcc
     4441 tgtaatgtgt tgtcaggcgt aattacagtt acatcatggc ctctgctcag caattccagg
     4501 cctaactcat gaaacatttt agccccaact cgtgtgctat ggggcaaata atcatcaatg
     4561 attaatgcaa gtttcattag tattgcttcc agacaacccg tttaacgtag tcagtatatg
     4621 aatggataat acgcacaact ttatctgaaa catttggcat actatagtca ctgacctgac
     4681 gaagtaagcg tacttctcca cgaggctgtg ttgcaataat ttctaatgcc tgtaacacac
     4741 gggcagactc aagaccaacc atcattacag ccccttcttc gaagccttca gggcgttcgt
     4801 gcgcttctcg tatattgagc gcagggaagt tcataatgga ggactcctct gtaatagtcc
     4861 cgctatccga taaaacagca cgtgcatttt tttgcagatg gttgtaatcg tgaaatccta
     4921 atggcttaag tagcaagata tttttatgaa attgaatacc gttttcgttg atgcggttac
     4981 gagtgcgcgg atgagtagaa acaactaccg ggatatcata tttttcagcc acggtattaa
     5041 gtatatttgc cagtttcgca agctgtttag gggtatcaac attttcttct ctgtgggcac
     5101 ttaccacaaa gaaattccca ggtgttaaat tcaggcgaga aagtacatcg gaaccatcaa
     5161 tctgcgacat ataatgagtg agtacttcaa acattgggct accggtttta ataatcctat
     5221 cggctggtac accttcagcc aaaagatatt cacgcgcgat atcactatat gtcatattga
     5281 tatcagcggt gtggtcaact atttttctgt tagtttcttc cggtacgcgt tgatcaaaac
     5341 aacgattccc agcctccata tggaaaatcg gaattcttcg acgctttgct ggtattgctg
     5401 aaatacagga gttagtatcg ccaagaacta acatggcttc tggtttttcc tgttcaaaga
     5461 cctcatcaac tttaatgata acttgtccaa tagtctctgc tgcattttta cctgcggcat
     5521 taagaaaata atcaggtttg cgaacaccca aatccttgaa aaaaacctca ttcaattcat
     5581 aatcgtaatt ttgcccagta tgaacaataa ggtgatcaca atattcatct aattttgcaa
     5641 ggacacgcga gagtcgaata atttctggac gagtcccaac aaccgacatg actttcaatt
     5701 ttttcacgat aaaactctcg ctatagtatc tggttcagaa cgattaaata tttcatttgc
     5761 ccaaagcata acaactagct catccgagcc attattagta atgtcatgcg tccatcccgg
     5821 aactgtttca acaattttaa aatcatctga tgaaacatta agttcatatc gttcacccgt
     5881 gacaatattt tcaaatttga aacaagcact tcctcggatg ataataaatt tctcattttt
     5941 ggaatgatga taatgccccc cccgagtaat tcctggatgc gcagtaaaaa acgaaaactg
     6001 gcccgcgttt ttcgttttca atacttcaca gaatacccct ctgtcatcac tataagaagg
     6061 aaccgtatac gcaaactgtt taggagaaag gtaacttaac catgttgagt acaatgctcg
     6121 agtgaatcca ttacctacat cttcgatgat taatgtttgg cgactttctt taaatcgata
     6181 aattaattgt gccacctcac caacagtaac agaataaatt ggaccaaatg tcctgtaacc
     6241 agtttcgttc gctccttcta atagctttaa tatgtcagaa caaaagtcat ctatatacac
     6301 caaatttaca actgctgaag gatcattaat tgtaatagct tcatcatttg caatgcgatg
     6361 gcagaaagtt gctataaagg agttataatt tggttgacac cacttaccga atacattcgg
     6421 caagcgataa atataatatt tagcgtttgt cgtttcccca tactgctgaa tgattttttc
     6481 cgcaactgct ttactctttc cataagcgtt atcacattca gcttggatgg atgaactaag
     6541 catgatagga gtatctttat tatttttttt cagaatatca acaatctgtt gagttagatc
     6601 gcaattcccc tcttcaaact cactctcctt cacaggacga tttactccag caagatgaaa
     6661 aatgaaatct gcctgcttaa gtccctgctc taaatccgtc aaagaagagt tacgatctat
     6721 cgcaataagt tcgttatatc cagcttcctt aaggcggaaa accaaatttc gaccgataaa
     6781 ccctgcagcg ccagtaacta aaattttcat attatgaatc caactcataa tcttcaccag
     6841 aacgaagtgc ccggataaaa ggaagtttta gcagtaattc tttcattccc tcaacatcta
     6901 atctctcagt attatgagaa ttataatctt ccacttccga gatacgacgg tcaccatgtt
     6961 ccacatattt tccatagttc aaatcgcgga gatctggtgg aacacgataa taatcaccca
     7021 tatcttccgc tgcaatcatt tcctctcggc tcaataacgc ttcgtaaagt ttttccccgt
     7081 gtcgagttcc aataatattg attggatgct catgggcatt tagcaattcc ttaagtgcaa
     7141 tggctaatgt ttgaattgtt gccgcaggtg ctttctgaac gaaaatgtca ccattatttc
     7201 catgttcgaa agcataaagg accagatcga cagcatcctc aagcgtcatc atgaaacgag
     7261 tcatattggg atcggttatg gtcaatggtt taccagcttt gattagatca acaaataatg
     7321 ggatgaccga tccacgtgaa gccattacat ttccataacg agttccacag ataactgttt
     7381 ttgaactgtc aagattacgt gattttgcaa caataacttt ttccatcatt gccttagata
     7441 tgcccattgc attgatagga taaacggctt tatcggtact caagcacacc acgcgtttaa
     7501 tcccattcgc aatagcagcc tccagaacat tttccgtacc cagaacattt gtcttaaccg
     7561 cctccatagg gtgaaattca catgaaggaa cttgtttaag ggctgctgca tgatacagaa
     7621 aatcggcacc acgcgttgca tttagaacgg aattatagtc tcgcacatca cctatataaa
     7681 attttaattt tgagttatta tattttttcc gcatatcatc ttgttttttt tcatccctgc
     7741 taaatattcg tatttcagta atatctgtat ctagaaaacg cttaagtacg gcattaccaa
     7801 aagaaccagt gccaccagtg ataacgagtg ttttattctt aaacatattt tca
//
LOCUS       DAZKGS010000191         7852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKGS010000191 DAZKGS010000000
VERSION     DAZKGS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(175..660)
                     /gene="sixA"
                     /locus_tag="ACHOQY_004248"
     CDS             complement(175..660)
                     /gene="sixA"
                     /locus_tag="ACHOQY_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9680223.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(863..3007)
                     /gene="fadJ"
                     /locus_tag="ACHOQY_004249"
     CDS             complement(863..3007)
                     /gene="fadJ"
                     /locus_tag="ACHOQY_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9680224.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3007..4317)
                     /gene="fadI"
                     /locus_tag="ACHOQY_004250"
     CDS             complement(3007..4317)
                     /gene="fadI"
                     /locus_tag="ACHOQY_004250"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9680225.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4497..4781)
                     /locus_tag="ACHOQY_004251"
     CDS             complement(4497..4781)
                     /locus_tag="ACHOQY_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9680226.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5153..6493
                     /gene="fadL"
                     /locus_tag="ACHOQY_004252"
     CDS             5153..6493
                     /gene="fadL"
                     /locus_tag="ACHOQY_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9680227.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6858..>7852
                     /gene="yfdF"
                     /locus_tag="ACHOQY_004253"
     CDS             6858..>7852
                     /gene="yfdF"
                     /locus_tag="ACHOQY_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFP9680228.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNR"
BASE COUNT         2097 a         2006 c         1805 g         1944 t
ORIGIN      
        1 taactttggg caaaatcaag atacgcgcag aagaataatt tccgaatgaa agtagaaagt
       61 gttttattta tagatgggga gagtcttcga tagtagtgag gttgacagat atccgctgcc
      121 tgatgagcaa ttatgcccaa tgaatctacc tcattgggca taatttggaa ctcatcagat
      181 agcttttgcc atcttcagat tacacgggct catctgccag ttgaatgtgc cgtttccgct
      241 ttcatcaagc gttacgctgg caatggccga ggtggtaaac ataggcggcg tttcgcccgg
      301 acataactcg gcaaccagat acccgactaa cggtaagtga gagatcacca gtaccgaagc
      361 aacaccttca ttggtcaacg cctgcaaata ggcactgacc agaccaacat cgccgcaggg
      421 cgttaactcc ggcagaactt ctgcactgga aggcaggttc agacaatccc ctacttcttc
      481 cagtgtttgc tcggctcgca ggaacgggct caccagaacg cgttcgattt ccactttttg
      541 acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa ccattagtgg tcagaggacg
      601 aacggaatca ctggcggcat cgagggctgc gtcgccgtga cgcatgataa aaacttgcat
      661 attgcaccgc ttttgttaac cagattcgcc cgttttcttt catctgaaac cagagaaaga
      721 aaacggtggc cggcattgtg ccttatccat tcaccgaatg aaacgctgtt ttttacctca
      781 atggcgtaag tatagtcaat atgcgtttat attttgacca acactccgcc attcagcgcg
      841 gattcatata gctttgacct tcttattgca ggtcagttgc agttgttttc caaaaacttt
      901 ccccacgcgc gcccatctcg accaaacgct cgcaaggggt aaaacgggaa ccatactgcg
      961 tggcaagtcg ttgcattatt gcaaccactt cgcccgcgcc gagagaatcg atatagcgga
     1021 acggtccacc gagaaatggc ggaaaaccaa tgccaaatac cgcaccaata tccccgtcac
     1081 gcacgctacg gataacctgc tcatcaacac aacgtactgc ttcattcagc atcaacatca
     1141 cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg cccttgtgcg ccaatgagcg
     1201 ggtaaatggc gggatcgacc tgttttttgc ttttacgccc tttctgacca taaagataga
     1261 aaccccgacc attttttctg cctttgcgat cgtcgttcaa aattgaagaa acaacatttg
     1321 caggcgcgct aaaacgttct ccataagcgg cttccagtac aggaataatt ttagtcccgg
     1381 tgtcgattcc tacctcatcc aaaagttgga ttggacctac cggaaaacca aatttcacta
     1441 gcgcggcatc aatgtgctct acccgttcac cttcggtcag catgcggata gcttcattaa
     1501 tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt gtcacgcacg acaatgggcg
     1561 ttttgccctg ttttttcgcc agttttaccg tagtggcgat ggtttgcgcc gatgtacccg
     1621 catgaggaat aatctctacc agcggcattt tttccaccgg actgaagaaa tgcaggccaa
     1681 taacttgctc aggtcgcgtg gcgtgagctg cgatatcacc aatcggtaaa gatgacgtat
     1741 tcgaagcaaa aatggtatga gcggcgcaat tttgctccac ttccgccacc atctgttgtt
     1801 ttaattcgag attttcaaac accgcttcaa taatcagatc gcgatgggca aagccgcgat
     1861 agtccgtcgt tccggagatt aatgccagct gtttgtcacg ttcgctggct ttgagatgac
     1921 gacggcgaac tttgccctcc agctgatccc aactgtactt cagcgcatga tttatgccct
     1981 gcgggttgat atctttaatt ctgaccggaa gccccgcttt acaagcagtg acataagcaa
     2041 taccgccgcc catcaagcca ccacctaaaa ttcccacgct gtttaatggc gcaggcggtg
     2101 catcactacc gggatctttc ttcacttccg tactggcaaa aaagatacta cgcaaagctt
     2161 gcgattgtgg cgtcatcgcc agttcgccaa acgcccgagc ttcggcgtca taaccgctgc
     2221 tggtgccctg cgctaatccc gtttcaacaa cctccaggat gcgttctgtc gccggataat
     2281 tgccttgagt tttgtgttct gttttcttgc cgaccatttt gaacagcagc gcacgaccta
     2341 acggccccgc cagaatacgc tcgcgtacag gtagagggcg ggaagatggg cgatccttct
     2401 tcgccagctc aacagcgact tccagcaaaa tggagtgcgg aacaacgtca tccaccagcc
     2461 ccagctttaa tgcctgtttc gcccgaagtt gttttccggt gaggatcatc tctaatgctg
     2521 tgctgacgcc tatcagacgc ggtaaacgct gggtgccgcc tgaaccgggt aacaatccaa
     2581 gttgtacttc aggcaaaccg agcaccgttt taggatcgtc agtacaaacg cgaccgtggc
     2641 acgccagcgc caactccagc ccaccaccca ggcaagcgcc atgaatagcc gcgataaccg
     2701 gaatgggcaa agcatgaatc tccgccatca actgttggcc ctgccgcgcc agtgcttccg
     2761 cttcttgcgc cgttttgcag ttgccgatca tgttgatgtc tgcgccagca atgaagttgt
     2821 ccggtttagc ggagacaaac accacgcctc gcaactcttt gttttcacgg agttgcttaa
     2881 taatggcgcg cacctgcgag gcaaactccg ccttcagggt attcattttc tcacccggta
     2941 cgtcgatggt gataacggca atgttgtcca gacgaacatt aagggtaaac gctgatgtca
     3001 tttccattat tccgcctcca gaaccattgc cgcgccaagc ccaccggcag cacaggcggt
     3061 aactaaacca aatccaccgc cgcgacggcg aagttcatgc aatgtctggg taatcatccg
     3121 tgcgccggtc gccgcgaagg gatgtccgta agcaatcgaa ccgccaagca cgttaaattt
     3181 gctatcgtcc acttcgccag tggcatgtgc acgccccagt acttcacgag caaaacgttc
     3241 actacccagc aactgaatat tcgccagtgt ctgagctgca aaggcttcgt gcatatcgat
     3301 caatgtcaga tcgctcattg tcaaaccggc acgttccagc gccagcggtg ttgaccaggc
     3361 cggaccgagc aacatgtcct gccagacatc aatcgcagta aatgcgtagc tgcgcagata
     3421 ccccagcggc accagcccta attctttcgc ccgggattca gtcatcagga tcaccgctgc
     3481 cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc gttccgtgtt tgcgatcaaa
     3541 cgccgggcgc agctttgcgt aatcggcaag cgaggaatta ccgcgaatat tgttgtcttc
     3601 gacaagcggt tgtttataag gagggataaa ggcagtcatc acctcttctt tgagttttcc
     3661 gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc gctaatgcat cttgctgttc
     3721 tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg tcgcccatcc gcaagccagt
     3781 agaatattct gctaccgcag gcggtacggg cattaagtcg cgcaaacgca ggcgagagaa
     3841 gagtttcagt cgctggctca tggttcgagc tttgttgaca tcaaccagca cgcgcgccag
     3901 ttttttactg acgccaattg gcaatacgga agaggaatct gccccaccgg caatccccgc
     3961 tcggatagtt cccgccatca ggctttctgc gacgtttgca accgcctgga aactggtagc
     4021 gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc attccggtac cgagaacaat
     4081 ttcacgcgca atgttggggg cttcaggcat ttgtacgacc tgaccaaaga ccagctgttc
     4141 aatcacttca gcggggatct cgctgcgtgc cagcagttcg cctaccacca tcttccctaa
     4201 atcaaccgcg ggaatgccat gaaaagccgt cgcctggcgg gcaaaaggcg tacgtaaacc
     4261 gctaacaatg gcgatacgat cgccctggcg ggtaaccagc ggtaaaacct gacccataac
     4321 actcccctgt aaaaaaataa ataaagtggt ctgacctgat catagtctta accatttttt
     4381 tacatttagc caagcggaga aaaaggaaag tgggagctat gacacagtga agacaggaga
     4441 aagaaaagaa aacgcccctg ccagcaactg acaggggcgt acgtacccga gaggaattaa
     4501 cgcagaccca gctggaagat cagcatttca gcttcgcagg caaaagtgaa atcgatatcc
     4561 aggcgcacac cgtcagactc ttcagtgaaa gtcggggtga ttttacacgg ttcggattcc
     4621 acgctacggg ctttttcagt cagcgccgcc agcgtttgtt ctgcttctgc gcggcttgca
     4681 aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc aacatccata
     4741 cagcagcaaa ccggggtttc atcagcacta catttactca tcgttgattt cctctgtatg
     4801 tgcacccaag gtgccagata aacgttgtgg atattttacg cttccggaaa gtgctgctcc
     4861 agttgttaat tctgcaaaat cggataagtg accgaaatca cacttaaaaa tgatctaaaa
     4921 caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt ttgccagctg gatcgcgttt
     4981 cttagatcat atttgaaaaa agatggaaac atacttgcaa cattccagct ggtccgacct
     5041 atactctcgc cactggtctg atttctaaga tgtacctcag accctacact tcgcgctcct
     5101 gttacagcac gtaacatagt ttgtataaaa ataaatcatt gaggttatgg tcatgagcca
     5161 gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg gcacttatct ccacccaggc
     5221 ctggtcggca ggctttcagt taaacgaatt ttcttcctct ggcctgggcc gggcttattc
     5281 aggggaaggc gcaattgccg atgatgcagg taacgttagc cgtaaccccg cattgattac
     5341 catgtttgac cgcccgacat tttctgcggg tgcggtttat attgacccgg atgtgaatat
     5401 cagcggaacg tctccgtctg gtcgtagcct gaaagccgat aacatcgcgc ctacggcatg
     5461 ggttccgaac atgcactttg ttgcaccgat taacgaccaa tttggttggg gcgcttctat
     5521 tacctctaac tatggtctgg caacagagtt taatgatact tatgcaggcg gctctgtcgg
     5581 gggtacaact gaccttgaaa ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa
     5641 tgcatggagc tttggtcttg gtttcaacgc cgtctacgct cgcgcgaaaa ttgaacgttt
     5701 cgcaggcgat ctggggcagt tggttgctgg tcagattatg caatctcctg ccgggcaaac
     5761 tcctcaaggg caagcattgg cagctaccgc caacggtatc gacagtaata ccaaaatcgc
     5821 tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc ggaatcctgt atgaactgga
     5881 taaaaataac cgctatgcac tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa
     5941 ctacagcagc gatcttaatc gtgcgtttaa taactacggt ttgccaattc ctaccgcgac
     6001 aggtggcgca acgcaatcgg gttatctgac gctgaacctg cctgaaatgt gggaagtgtc
     6061 aggttataac cgtgttgatc cacagtgggc gattcactat agcttggctt acaccagctg
     6121 gagtcagttc cagcagctga aagcgacctc aaccagtggc gacacgctgt tccagaaaca
     6181 tgaaggcttt aaagatgctt accgcatcgc gttgggtacc acttattact acgatgataa
     6241 ctggaccttc cgtaccggta tcgcctttga tgacagccca gttccggctc agaatcgttc
     6301 tatctccatt ccggaccagg accgtttctg gctgagtgca ggtacgactt acgcatttaa
     6361 taaagatgct tcagtcgacg ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa
     6421 cgaaggccca taccagttcg agtctgaagg taaagcctgg ctgttcggta ctaactttaa
     6481 ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc tgcatagcag gtgactttaa
     6541 ctcccccact tcaccgaaag tagtgtcctc attgcttacc tcctgagttt tgcaaacagc
     6601 ctgatggcag cttgcccttt caaatcaata agcggtatcc acagacaaat ctgaagggaa
     6661 aggcatattt tcaggcgttc tgctcgtcct tctcaaagag tttacttttc tgaatttcca
     6721 ggatactcac ccccctggct attgtgcgct catacactca aattaaagat aggttctaaa
     6781 taaatgagcg ttttttgata gtctatttca ttaggtaata tatatttgta acaaatcaat
     6841 caaaatggaa taaaatcatg ctaccatcta tttcaatcaa caataccagc gcaacttacc
     6901 cagaatccat taatgaaaat aacaatgatg aagttaatgg attagtacaa gagctcaaaa
     6961 acctttttaa tggtaaggaa ggaataagca cctgtattaa acatctactt gagcttataa
     7021 aaaacgccat acgagtaaac gacgatcctt atagatttaa tattaataat tcctcagtta
     7081 cttatattga tattggctcc aatgatacag accatattac tattggtatc gacaaccaag
     7141 aaccaataga attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg
     7201 acaacatagt tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa
     7261 aagaaatata tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac
     7321 atacggtaac ggagtttgat gagagcggga atagcgaacc aacggactta tttacctggt
     7381 acggtaaaga taaaaaaggc gactctctcg ctattgtaat taaaaataaa aacggaaatg
     7441 attacttatc tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta
     7501 ttaatggtga tagtctcacc caatattgta gtgaaaaagc caggaatgct tcagcgtggt
     7561 ttgaaagcag taaagctatc atggcagaat catttgcaac aggttccgat catcaggttg
     7621 taaacgagct caacggggaa agactgagag aacctaacga agtttttaaa cgtttaggtc
     7681 gagcaataag atataatttt caagtggacg atgcaaaatt tagacgcgac aatgtaaaag
     7741 aaataatttc taatttattc gctaacaaag ttgacgttga ccatcctgaa aataaacaca
     7801 aagactttaa ggatctcgaa gataacgttg aaaaaagact tcaaaatcgc ca
//
LOCUS       DAZKGS010000192         7834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKGS010000192 DAZKGS010000000
VERSION     DAZKGS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..546
                     /locus_tag="ACHOQY_004254"
     CDS             22..546
                     /locus_tag="ACHOQY_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFP9680229.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            630..1316
                     /gene="lpfB"
                     /locus_tag="ACHOQY_004255"
     CDS             630..1316
                     /gene="lpfB"
                     /locus_tag="ACHOQY_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP9680230.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1340..3871
                     /gene="lpfC"
                     /locus_tag="ACHOQY_004256"
     CDS             1340..3871
                     /gene="lpfC"
                     /locus_tag="ACHOQY_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP9680231.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3882..4937
                     /gene="lpfD"
                     /locus_tag="ACHOQY_004257"
     CDS             3882..4937
                     /gene="lpfD"
                     /locus_tag="ACHOQY_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP9680232.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4943..5467
                     /gene="lpfE"
                     /locus_tag="ACHOQY_004258"
     CDS             4943..5467
                     /gene="lpfE"
                     /locus_tag="ACHOQY_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP9680233.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5720..7411
                     /gene="eptB"
                     /locus_tag="ACHOQY_004259"
     CDS             5720..7411
                     /gene="eptB"
                     /locus_tag="ACHOQY_004259"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9680234.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7503..7579
                     /locus_tag="ACHOQY_004260"
     tRNA            7503..7579
                     /locus_tag="ACHOQY_004260"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7537..7539,aa:Pro,seq:cgg)
     gene            7614..7747
                     /locus_tag="ACHOQY_004261"
     ncRNA           7614..7747
                     /ncRNA_class="other"
                     /locus_tag="ACHOQY_004261"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2037 a         1804 c         1988 g         2005 t
ORIGIN      
        1 tctatataaa tggagttata aatgaaaaag gttctgtttg ccttatctgc gcttgcgctg
       61 acctctactt ccgtattcgc agctgatgcg ggcgacggta ctgttaaatt caccggtgag
      121 attgttgacg ccgcttgtgt tgtgtctcca gacactcaaa aacaagaagt tgttctgggt
      181 caggtgaaca agtctgtatt tactgctatg ggtgataaat ccacagcaac gccgttcaaa
      241 atcaaactgg aaaactgcga tatctccact ttcaaaaatg tggaaatcag ctttaacggc
      301 gttggcgatg cagacaacag caaactggtt tctgtaagca ctgaaccagg tgccgcaact
      361 ggcgtaggta tcggtattta tgataacacc aataccctgg ttgacctgaa tactggtaaa
      421 tctgctactg tcctgaaaga aggtcagacc gttctgtatt tcaccgctaa ctatgtctct
      481 accaaagatg cagtaaccac gggttacggt aacgctgaag tcgacttcaa cctgacttac
      541 aactaatcga tcctgacgga acgatcatct ctcaatggca acagagactg tgttgccatt
      601 attcacacca atgactcagg gaccgaatca tgaaacgtat tagctttgct gtgctggcac
      661 ttttaatgac cgcacaacag tgttttgctg gtggcgtagc cttaagcacg acgcgtgtta
      721 tttatgatgg cggcaagaaa gaagcgtcgc tgaccgttga aaaccataat aaaaatgaag
      781 agtttttgat ccagtcatgg attgatgatg tgaatggcaa taaaaaaaca ccgtttatta
      841 ttacaccgcc attattcaaa cttgatcctg ataaaaataa cgtcttacgt atcgtcaata
      901 ttgaagatac cttacctaaa gatcgtgaat ccgtttactg gattaatgta aaggccattc
      961 ctgcgaaaag tgctgaaaat gaaaataaaa acgtcttaca gatagccgtt cgcacccgtt
     1021 tgaagttgtt ctatcgtcca aaaggtttgc cgggaagcaa cctcgaggcc atgaaacagt
     1081 tacgtttcca gcgtcaaaat aaccaaattg tggttaataa cgactccgct tataacctga
     1141 cctttaatca gttttccatt aatggacaaa agattgaacg ggcgggaatg gttctcgcga
     1201 aaggaaagtt aaacattaac cttccagccg gtacaggcaa cgcgcacgaa gtgaaataca
     1261 gcattattaa tgatttcggt gttgctggcg aaatgctgac gaagccgatt aactgatcct
     1321 tttacaaggc gcttttacta tgcattggat gcatttacct ctctatcact acagagcgca
     1381 tttttccttc tctctgctgg cattaacgat tgccagctcg ttacctgcgt atggcggaaa
     1441 atttaaccct aaatttctcg aaaacgttca gggtatcgat caacacatcg acttgtcggt
     1501 atacgactca cctgtcggcc agcaaatccc cggtaaatac cgggtttcgg tgtttgtaaa
     1561 cgaagaaaaa atggcttccc ggacgctgga ttttagtacc gccagcgaag cgaaacgcaa
     1621 agccagcggt gagtcgctta tgccttgcct tagccgggtg caactggaag aaatgggcgt
     1681 gcgcgttgac agctttccgg cgctgaagat gtcaccacca gaagcctgcg tagcgtttga
     1741 tgaaatcatt ccccaggcca ccagccgctt tgattttaat actcagacgc tgcatctttc
     1801 ctttccacag gcggcaatga tgatgaccgc tcgcggcacg gttgatcctt ctcgctggga
     1861 cgaaggaata cctgcgctgt tgctggatta cagctttagt ggcagcaatg gacgcaatga
     1921 aggtacaggt tcatcttcag attcgaccag tgacagttat tacctgaacc tgcgcagcgg
     1981 tttgaacgtt ggtccgtggc gtttacgtaa taacagtatc tggaaccgta ccgatggcaa
     2041 aaaccaatgg gataacgttg gtacatcgtt aaaccgggca attattccac ttaaatcaca
     2101 gataactctg ggtgataccg ccacgcctgg cgagattttt gatagcgtac aaatgcgtgg
     2161 ggcgttactg gcatccgacg atgaaatgtt gccggacagc cagcgtggct ttgctccggt
     2221 agtgcgcggc atcgccaaaa gcaacgctga agtgagtatc gaacagaacg gctatgtcat
     2281 ttatcgcact tttgttcagc cgggtgcatt tgagattaac gatctctacg caacctccgg
     2341 cagcggcgac ctgacggtga tcattaaaga agccgacgga agtgaacaac gttttatcca
     2401 gccgttttcg gcggtggcga ttttccagcg tgaagggtat ctgaaataca gtctggctgc
     2461 cggtgaatat cgtgctggta attacgacag cgataaacca catttcgggc agtttaccgc
     2521 catgtacggc ttgccgtggg gtatgaccgc ctacggtggt gcattgttgt cggcagatta
     2581 caacgcttta gctctgggcc tggggaaaaa cttcggtacc attggcgcag tatcggtgga
     2641 tgtcactcag gcgaaaagtc agttacgcaa caatgaaaaa gaggaaggcc agtcttatcg
     2701 cttcctctat tcgaaaagtt ttgagggtgg cactgacctg cgtctgttgg gctacaaata
     2761 ctcaaccagc ggctactaca ccttccagga agcaactgat gttcgcagtg acgccgacag
     2821 tgattaccgc cgttatcaca agcgcagcca gatacagggc aatattaccc agcaactggg
     2881 agactacggc tcggtttatt tcaatatgac gcagcaggat tactggaacg ttgacggtaa
     2941 ggaaaactcc ttaagcgccg gttatcacgg tcacattggt cgggtgaatt acagcgtggc
     3001 ctatacctgg acgagaagtc cggaatggga agaagacgat cgcttgtggt cattcagtgt
     3061 ttccatccct ctggggggcg cgtggagcag ttatcgcatg actaccgacc agaatggcaa
     3121 gacgtcgcaa caggcctctg ttagcggcac gttactggaa gatcgtaacc tgagctataa
     3181 cgttcagcag ggttacacca gtaatggcgt aggctacagt ggcagcgtca acatggggta
     3241 tatgggtggc tctggtaaca ttgacgtagg ctataactat tctaaagata accaacaggt
     3301 taactatggc gtgcgcggcg gtgtcatcgt tcacagtgaa ggtataacgc tttcgcagcc
     3361 tttaggcgaa agtctggcga ttgtttctgc accgggcgcg cgtggtggac atgtggttaa
     3421 cagcagcggc gtagaagttg actggatggg caacgccgtg gtgccttatc tcacaccata
     3481 tcgtgaaacc attgtggaat tgcgtagcga cacgttgggg caaaacgttg aactacagga
     3541 agcgttccag aaagtcgtgc ctactcgtgg tgcagttgtg cgttcccgct ttgatacccg
     3601 cgtcggttac cgtgtgctga tgagcttaaa acaggcgaat ggcaacgcag taccgtttgg
     3661 tgcaaccgca gcgttaattg atgagagcaa acctgccagc agcattgtgg gtgaagaagg
     3721 acagctttat atcagcggga tgccagaaga gggcgaattg caggtgagct gggggaacga
     3781 gcaggcgcaa cgttgccgtg tgccattccg cttgccagag aacaaggaca atgcggctat
     3841 cgtgatggtt aatgcggtat gcgagaaata aggatattta gatgaaaatt aaaaatttgt
     3901 tggcaggtat ggtgctgtta gggacgagca cttttgctgg aaatatatgg gctgcggatt
     3961 ggggaccatg tcagacatca aatggggtgg cccatgagta tagttttgat ttcgtacaga
     4021 ctatccaggt accttcagaa aataaggctg ggaaaatatt aactcagccc tttgctttag
     4081 gtacaaaata ttccgcttat tgtgaatgcc cggatcccat tccagataat ggagttgtga
     4141 cttattttaa aggtgtaacg cttctgcctg agcctggaac tgtagatggg tactataaat
     4201 tcaataatta cattgatatt ctcaccaaaa tatatgtcta cacacaatta gatatcccgg
     4261 tgccttttac cgatcgaagc aatggcacgg cacagaaaga atgtacgccc tatactgcta
     4321 acaattgggg gacaggtggt aaaggaagta tcagtattta tatttcacat ccttttgttg
     4381 ggcaaatgat tattcctaaa acacgagttg cgtccctctt tgggacaaaa aagaaaggtg
     4441 tttacaatga tagtcagccc atggccaatg tcagtatttc cgggagtatt acagttactc
     4501 aaggctgtga acttgccgct ggcaccgtac tggatattcc attcggagaa tatcaggccc
     4561 atgattttaa agggcgtgcc ggacaaccac cacaaaatgt tcagaaggta caaaaagaat
     4621 taagctttga ctgcaataat atttcagatg gcgtcaaaat ttatctcagt attgatgcaa
     4681 caccaaatac tgcctatccc agcgcaattg atttaggcaa cgccgatgtt ggtgcagtca
     4741 ttgaggacgg taaaggtaat attcttaagc cgaatgacag taatagctta ttagaaatga
     4801 acccaggctc gctgtatgaa gatgtcaaac gcaaagcgac aaccacaatt accgcttatc
     4861 ccgttagcac taccggaaaa ttacccgctg ccggggatta tagcggtatt gctaccatac
     4921 atgtcgaact ggagtaacag tcatgaagcg aacaatactt gcgttatgcc tgctgcctct
     4981 ttccttttcg tcattcgcgg caaccacgga tttagggccg aaaggaacat tgaaattcac
     5041 ccttaaaatc agccagggtg cttgcgaact ggtgaaagac agcgttgagg ttgacatggg
     5101 caccgcggtt ctcaaaaagc cggtaagaat cggaacggaa attaatccca ctcctttttc
     5161 catcggcctg aaaaactgta gcgaagtggt cagagcctac gtgactatgg acggtacacc
     5221 caatgcagac gactcaaatc tttttgcgct ggatgcgggt ggcgctacag gcgttgggct
     5281 gaaaatcaaa accgctgctg gagtacaaca gttcccggaa aatacaacgc cgacaccggt
     5341 tgagtttgca attaacggtg atggcgcaca tcaactgaac tatatcgcca gctacgtgcc
     5401 tattcgcgcc gatgcaacta ttggtcgcgc agatgcaacg gtcgacttta ctgtacagta
     5461 cgaataattt cacatcttaa gggccagtta actggccctt tttactgctt tttgtaacgc
     5521 taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc
     5581 acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact
     5641 acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt
     5701 ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt
     5761 tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg
     5821 cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc
     5881 caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg
     5941 gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt
     6001 ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct
     6061 gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc
     6121 tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc
     6181 agggcagcgt attcgcagcc tggcggtcgt cgtactggcg ggtattatgg tttgggcacc
     6241 gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc
     6301 gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg
     6361 gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc
     6421 gaagaaattc acttatcagg caccgcaaaa cgttgatgac acttatgtcg tgtttatcat
     6481 cggtgaaacc acgcgttggg accatatggg cattttcggc tatgagcgta ataccacgcc
     6541 gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac
     6601 caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg
     6661 cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct
     6721 ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcggaca acattgctta
     6781 tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct
     6841 ggtagacgaa atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct
     6901 gcataccaaa ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg
     6961 gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta
     7021 tgacaactcg gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga
     7081 taagaaagcg attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca
     7141 cctgcacggc acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt
     7201 ctggatgtca gataaatatc tggaaaatcc ggccaatgct caggcatttg cgcagctgaa
     7261 aaaagaagcc gacatgaaag tgccacgccg tcatgtagag ctgtacgaca ccatcatggg
     7321 ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat
     7381 cccgcagaca aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa
     7441 aggcattttg ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca
     7501 ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg
     7561 aatcctctat caccgaccaa attcgaaaag cctgctcagc gagcaggctt ttttgcattt
     7621 gaagcccatg aggatgagaa cctccggggg cggggaggtt cgactcgagc gaagcgagag
     7681 aacgttgcgc cagcaacggc ccgcagggcg agccacgaag tggcgagtaa tcctctatca
     7741 ccgaccaaat tcgaaaagcc tgctcaacga gcaggctttt ttgcatttgt agctcatgag
     7801 gatgagaacc tccgggggcg gggaggttcg actc
//
LOCUS       DAZKGS010000193         7805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKGS010000193 DAZKGS010000000
VERSION     DAZKGS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..1088
                     /locus_tag="ACHOQY_004262"
     CDS             36..1088
                     /locus_tag="ACHOQY_004262"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9680235.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1164..2597
                     /locus_tag="ACHOQY_004263"
     CDS             1164..2597
                     /locus_tag="ACHOQY_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9680236.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2780..5041
                     /locus_tag="ACHOQY_004264"
     CDS             2780..5041
                     /locus_tag="ACHOQY_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9680237.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5275..6558)
                     /locus_tag="ACHOQY_004265"
     CDS             complement(5275..6558)
                     /locus_tag="ACHOQY_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9680238.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6693..7763)
                     /locus_tag="ACHOQY_004266"
     CDS             complement(6693..7763)
                     /locus_tag="ACHOQY_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9680239.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTH
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1852 a         1868 c         1988 g         2097 t
ORIGIN      
        1 gcaataagtt atgtttttaa attgagggca ttattatgaa aaaaataccc tgcgtgatga
       61 tgcgaggtgg aacctcgagg ggcgcgttcc tgttagcgga acatttaccc gaagatcaaa
      121 cgcagcgcga taaaatattg atggcaatta tgggttccgg taacgatctg gaaattgacg
      181 gtattggcgg cggtaatcca ctgaccagta aagtcgccat tattagccgt tccagcgatc
      241 cgcgtgctga tgttgattat ctgtttgctc aagttatcgt ccatgagcaa cgtgtcgata
      301 ccacgcctaa ctgcggcaat atgctttctg gcgttggggc attcgccatt gaaaatggtt
      361 tgattgcagc gacatcaccg gtcacccgtg tgcgtatccg caacgtcaat acgggtacgt
      421 tcatcgaagc tgatgtgcaa acgccaaatg gtgttgtcga gtacgagggt agtgccagaa
      481 ttgacggcgt accgggtact gccgcaccgg ttgcgctcac tttcctgaat gccgctggaa
      541 ccaaaaccgg aaaagttttc ccgactgata atcagattga ttattttgac gatgtcccgg
      601 tgacctgtat cgatatggcg atgccagtcg tcattattcc ggctgaatat ctgggaaaaa
      661 caggttatga attaccggcg gaactggatg ccgacaaagc attattagcc cgcattgaat
      721 ctatccgtct acaagcgggt aaagcaatgg gcttaggtga tgtcagtaat atggttatcc
      781 ctaaacctgt gcttatttct ccagcgcaga aaggcggggc aattaatgtg cgttatttta
      841 tgccgcattc ttgccatcgc gcgctggcga taaccggtgc tattgctatt tccagtagtt
      901 gtgcattgga aggcaccgtc acccgacaaa tcgtcccttc tgtaggatac ggcaatatca
      961 atattgaaca ccccagtggt gcgctcgacg ttcatttaag taatgaaggt caggatgcca
     1021 cgacgttacg cgcatctgtt attcggacga ccagaaaaat attttccggt gaagtttatc
     1081 ttccctgaaa aaattcgttg tcaagataag gataatcaat aaaggacttc tgtatgagtc
     1141 atacagaaag aacaggattt taaatgaata agaaatcgtt atggaagcta attctgatat
     1201 tagcgatccc atgtattatt ggttttatgc cagctccggc aggattaagc gaactggcgt
     1261 gggtgctttt tggtatttac ctggcggcca ttgtggggct ggttatcaag cctttcccgg
     1321 aacctgtcgt actgttaatt gccgttgctg cctccatggt ggtggtcggt aacttatccg
     1381 acggtgcgtt taagaccacc gccgtattaa gcggttactc ttcaggtact acctggctgg
     1441 tgttctcggc gtttacctta agcgccgcat ttgtgaccac cggtttaggt aaacgtattg
     1501 cctatctgct gattggtaaa atcggtaaca ccacgctggg tctgggttac gttacggtat
     1561 tcctcgatct ggtactggct ccggcaacac cgtctaacac cgcgcgtgcg ggcggtattg
     1621 tgttaccgat catcaacagc gtggcggtgg ctttggggtc cgaaccggaa aaaagtccgc
     1681 gtcgtgtcgg acattacctg atgatgtcca tttacatggt caccaaaacc accagctata
     1741 tgttctttac cgcaatggcg gggaacattc tggcgctgaa aatgatcaac gacattctgc
     1801 acctgcaaat tagctggggt ggatgggcac tggccgccgg attgccgggc atcattatgc
     1861 tgctggtcac cccactggtg atttatacca tgtatccgcc agaaattaag aaggtggata
     1921 ataaaaccat cgctaaagcg ggccttgccg aactgggacc gatgaaaatc cgcgaaaaaa
     1981 tgctgctcgg tgtctttgtg ctggcgctac tgggctggat tttcagtaag tctctggggg
     2041 ttgatgaatc cactgtggca atcgttgtta tggcgaccat gctgctgctg ggtatcgtta
     2101 cctgggaaga cgtggttaaa aataaaggcg gctggaatac cttaatctgg tacggcggta
     2161 ttatcggatt aagctcctta ttatcgaaag ttaaattctt cgaatggtta gctgaagtct
     2221 ttaaaaataa cctggcattt gatggtcacg gtaacgttgc tttcttcgtt attattttcc
     2281 tcagcattat cgtgcgttat ttcttcgctt ccggtagtgc ctatatcgtt gctatgttac
     2341 cggtatttgc catgctggcg aacgtctccg gcgcaccgtt aatgttaacc gcgctggcac
     2401 tgttgttctc caactcctat ggcggcatgg ttactcacta tggcggcgcg gcaggtccgg
     2461 tcatctttgg cgtgggttat aacgatatta aatcctggtg gttggtcggt gcggtactga
     2521 cgatattaac cttcctggtg catatcaccc tcggcgtgtg gtggtggaat atgctgatcg
     2581 gctggaacat gctgtaaata ttctcgtcat acttcaagtt gcatgtgctg cgtctgcgtt
     2641 cgctcacccc agtcacttac ttatgtaagc tcctggggat tcactcgctt gtcgccttcc
     2701 tgcaactcga attatttaga gtatatccat ttattatctt tctgcgcact tcacggtgcg
     2761 cagatatctg gagcatttga tgatcaagtt atctgaaaaa ggcgtgtttc tcgccagtaa
     2821 taacgaaata attgccgaag aacatttcac cggcgaaatt aaaaaagaag aagccaaaaa
     2881 aggcactatt gcctggtcta ttctctcttc gcataatacg tccggaaata tggataaact
     2941 taaaattaag tttgattcat tagcctctca cgatattacc tttgttggta ttgtacagac
     3001 cgctaaagcg tccggtatgg aacgtttccc gctgccgtat gtgctgacca actgccataa
     3061 ctcactctgc gccgtcggcg gcactattaa cggtgatgac catgtttttg gtttatcggc
     3121 ggcccagcgt tatggcggta tttttgtgcc tccgcatatt gcggtcatcc atcaatatat
     3181 gcgtgagatg atggcaggcg gcggcaaaat gatcctcggg tcagacagcc acacccgtta
     3241 cggtgcatta gggacaatgg cagtcggtga gggcggcggt gagttggtaa aacagctgct
     3301 taatgacacc tgggatatcg actatccggg cgtggttgcg gtgcatctga ccggaaaacc
     3361 tgcgccgtat gtggggccac aggatgtggc gctggctatc attggcgcgg tgttcaaaaa
     3421 cggttacgtc aaaaacaaag tcatggagtt cgttggaccg ggcgttagcg cgctctctac
     3481 cgatttccgt aacagcgttg acgtgatgac cacggaaacc acctgtttaa gctccgtctg
     3541 gcaaactgat gaagaagtgc ataactggct ggcgctgcac ggtcgcggcc aggattactg
     3601 ccagcttaac cctcaaccga tggcgtacta cgatggctgc atcagcgttg atttaagcgc
     3661 catcaaacca atgattgcgc tgccgttcca cccgagcaac gtgtatgaaa tcgacacact
     3721 gaaccagaac ctgaccgaca ttctgcgtga gattgaaatt gagtccgaac gcgtggcgca
     3781 cggtaaagcc aaactctcgc tgctggataa agtggaaaat ggtcgcctga aagtgcagca
     3841 ggggattatc gcgggctgtt ctggcggtaa ctacgaaaac gtcatcgcgg cggcgaatgc
     3901 actgcgcggt caatcctgtg gcaatgacac cttctcgctg gcagtttacc cgtcatcaca
     3961 gccggtgttt atggatctcg ccaaaaaagg tgtggtagca gatttgattg gcgcaggcgc
     4021 aatcatcaga accgcgttct gcggcccatg ctttggcgcg ggcgatacgc caatcaacaa
     4081 cggtttgagt attcgccaca ccacgcgtaa cttcccgaac cgcgaaggct ctaagccagc
     4141 taatgggcag atgtcagcgg tggcgttgat ggacgctcgt tctatcgctg cgactgcggc
     4201 aaacggtggc tatttaacct ctgccagcga acttgattgc tgggacaacg tgccggagta
     4261 cgccttcgat gtaacgccgt ataaaaaccg tgtttatcag ggctttgtga aaggggcaac
     4321 tcagcaaccg ctgatttacg gaccgaacat taaagactgg ccggaattgg gtgcgctgac
     4381 tgacaatatc gtcctgaaag tgtgctcgaa gatcctcgac gaagtgacca ccaccgacga
     4441 actgattcct tccggtgaaa cctcttctta tcgttcaaat ccgattggtc tggcggagtt
     4501 taccctgtca cgccgcgatc ccggttatgt tggcagaagt aaagcgactg ctgagctgga
     4561 aaatcagcgt ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca
     4621 gattgctggt caggagcata ttgatccgct gcaaactgaa attggcagca tggtatatgc
     4681 ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg
     4741 cggtctggcg aatattgccg aagagtacgc gactaaacgc taccgttcta acgtcatcaa
     4801 ctgggggatg ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat
     4861 ttacatccct ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt
     4921 gatccatgaa gatgcgccgg taacggaaat tacgctctat atggaaagtc tgactgctga
     4981 agagcgcgag attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta
     5041 aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgcttgcgc
     5101 atcttatccg acctacgaat cgcatcgaat ctgtaggcca gataaggcat tttcgcagca
     5161 tccggcactt attgtcggat gcgatgcttg cgcatcttat ccgacctaca aatcgcatcg
     5221 aaccgtaggc cggataaggc gtttacgccg catccggcaa atagttaatt gctcttactt
     5281 cttcgcctct gcaaccactt tactacccac gccgcggtta ttgtattccc acatgcggtt
     5341 gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta tcatctacgc tgccggtatt
     5401 acccgcaaac ggacgattag agatcaccgc atcggcccac ggtttagccg tgttaaaacc
     5461 ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg gtattggcat caacatccag
     5521 cgagcggccc agttgcgcca caccatcacc gaaagcattg aaacggctgt ttacggcgag
     5581 gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac acatacgctt cttgctgagt
     5641 acgtgagttc accacgcgga attcggtgtt atcgaacacc actgcgccgc gaccagaaac
     5701 gatatccaca tccccttcaa tgtagctgtt ggtcaccagc gtacgcggct gacgattcgt
     5761 ttccagacgg ttctgcacac cgctgttggt gacaaagaag gtgttctgac gaccgagaat
     5821 gttaacgttg ttaatctgta cctggtcacc atcagtacgc agtgccaccg ccggatggtt
     5881 acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc agattttgca gttgcaggcc
     5941 attgttttgt gaccagaaga ccgcagagca gagaacaccg atactgtcgc tgcgtttgct
     6001 ctggcagcta tcgtacatat accacgctgg tttacctggc atatatttgc cgcgcgggtt
     6061 gacgtcgtga cgccagtcgg cagggctcat gccaccatca agggaaagcc caatcttcac
     6121 atcaatcggt ttttcacctg taccgtacag agtaattcca cccggagcgg cagggacata
     6181 taccgttccc tgatactcac caggcatcac ggcaatatac tggcgcttgt tggtacgctt
     6241 gataattgcc gcatctaccg ccgcctgaat cgtggtatgc gttacacctt gagtgcccgc
     6301 cgggccgaca acaaagtcag gttgcgcagg cagggtaatc ggggaaggat tccacgctgc
     6361 tgcacctggt gtcagggatg caaaatagtg ttgagcatcg aaattctgcg cttcttttgc
     6421 cgacagaatc gggcgagaag aggtaccagg cgcggtttga tcagaaggac gttgatcggg
     6481 cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc aatgccagcg ccagacggga
     6541 aactgaaaat gtgttcacag gttgctccgg gctatgaaat agaaaaatga atccgttgaa
     6601 gcctgctttt ttatactaag ttggcattat aaaaaagcat tgcttatcaa tttgttgcaa
     6661 cgaacaggtc actatcagtc aaaataaaat cattatttga tttcaatttt gtcccactcc
     6721 ctgcctctgt catcacgata ctgtgatgcc atggtgtccg acttatgccc gagaagatgt
     6781 tgagcaaact tatcgcttat ctgcttctca tagagtcttg cagacaaact gcgcaactcg
     6841 tgaaaggtcg gcggatcccc ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa
     6901 taccttgata ctgtgccgga tgaaagcggt tcacgatgag tagatgcaat tatggtttct
     6961 ccgccaagaa tctctttgca tttatcaagt gtttccttca ttgatatccc gagagcatca
     7021 acatgcaatg ttgtagggat ggcaattttt acgcctgttt tgctttgctc gacataaaga
     7081 tatccatcta cgatatcaga ccacttcatt tcgcataaat caccaactcg ctgcccggta
     7141 acaacagcca gctccattgc aagtcttagc caacatggtg atgattctgc tgcttgataa
     7201 attttcaggt attcgtcagc cgtaagtctt gatctcctta cctctgattt tgctgcgcga
     7261 gtggcagcga ccgggtttgt tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg
     7321 ctcagtgttg atctgattaa cttggctgac gccgccttgc cctcgtctat gtatccactg
     7381 agcattgccg caatttcttt tgtggtgatg tcttcaagtg gagcatcagg cagccccctc
     7441 cttattgctt taattttgct catgtaattt atgagagtct tctgcttgat tcctctgctg
     7501 gcgaggattt tttcgtagcg atcaagccat gaatgtaacg taacagaatt atcactgttg
     7561 attctcgctg tcagaggctt gtgtttgtgt cctgaaaata actcaatgtt ggcctgtatt
     7621 gcttcagtga ttgctatcct cctgtctcgg cctaatccaa actctttacc cgtccttggg
     7681 tccctgtagc agtaatatcc attgtttctt atataaagat tagggggtaa atcccggcgc
     7741 tcatgacttc gccttcttcc catttctgat cctcttcaaa aggctacctg ttactggtcg
     7801 attta
//
LOCUS       DAZKGS010000194         7646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKGS010000194 DAZKGS010000000
VERSION     DAZKGS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(143..847)
                     /locus_tag="ACHOQY_004267"
     CDS             complement(143..847)
                     /locus_tag="ACHOQY_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9680240.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(984..1436)
                     /gene="moaE"
                     /locus_tag="ACHOQY_004268"
     CDS             complement(984..1436)
                     /gene="moaE"
                     /locus_tag="ACHOQY_004268"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9680241.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1438..1683)
                     /gene="moaD"
                     /locus_tag="ACHOQY_004269"
     CDS             complement(1438..1683)
                     /gene="moaD"
                     /locus_tag="ACHOQY_004269"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9680242.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1676..2161)
                     /gene="moaC"
                     /locus_tag="ACHOQY_004270"
     CDS             complement(1676..2161)
                     /gene="moaC"
                     /locus_tag="ACHOQY_004270"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9680243.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2164..2676)
                     /gene="moaB"
                     /locus_tag="ACHOQY_004271"
     CDS             complement(2164..2676)
                     /gene="moaB"
                     /locus_tag="ACHOQY_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9680244.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2698..3687)
                     /gene="moaA"
                     /locus_tag="ACHOQY_004272"
     CDS             complement(2698..3687)
                     /gene="moaA"
                     /locus_tag="ACHOQY_004272"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9680245.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3675..3819)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4084..4992
                     /gene="yvcK"
                     /locus_tag="ACHOQY_004273"
     CDS             4084..4992
                     /gene="yvcK"
                     /locus_tag="ACHOQY_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9680246.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5184..7205)
                     /gene="uvrB"
                     /locus_tag="ACHOQY_004274"
     CDS             complement(5184..7205)
                     /gene="uvrB"
                     /locus_tag="ACHOQY_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9680247.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1844 a         2029 c         1903 g         1870 t
ORIGIN      
        1 acaaaatact tttgtattta tggatacgga aaacgcttcc gctcgccata aggcgtattt
       61 tatacttggt acttgttaag tgtcgttttt agtaagaaaa aaaatcccga cacatggccg
      121 ggatattgat ggtgaaaaag aattaacggc ggttgccaaa aattcgcagc aacatcagga
      181 acaggttgat gaagtccaga tacaaggtta acgcgccaag aatggaatat ttgcgcaggt
      241 tcgacgtgtc gcgggtatca atctgctcac ccatattttt caatttctga gtgtcatacg
      301 ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac tgcccacatc aatgcttcgc
      361 ttttcagcca gaagttgacc agcgatgcca gcacaatgcc gattaacgcc ataaacagca
      421 tattgccaaa gccacttaaa tcgcgcttcg tggtgtaacc gtacaggctc attgcgccga
      481 acatcccggc agtaacgacg aaagtactgg cgatagaagc agcggtatag acaatgaata
      541 tactggaaag cgtaagaccc gtcagcgccg aataaagcat aaagagcatc gtcgttacac
      601 ctgcgctcag cttttgaatc atcgctgata acacaataac caatgctaat tgcgcgatga
      661 tcagaccgat taaaaagaca cggttagtaa acaacagctc catcacggcc gcggaattag
      721 ccgcatacca ggcaacaaat gcggtcagca acaagccaac ggtcatccag ccatagactt
      781 gcgccatata agtttgcaag ccagcccggg gttgtacgat tgaatcagaa cgtgggaatc
      841 tgtccatgac gatctcctga agatataagg aatatcttaa ggatactgca aaatgatgag
      901 gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt gaaattgccg gacgcagcgg
      961 tgccttatcc ggctaacaaa aaactaccag cgttttgccg cctgctgatc gctctcccga
     1021 gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct tccagaacgg tgcgcgggtt
     1081 ttgagataat ccataataaa ctgcccggct tcaaacgcac tgctgcgatg cgcactggtg
     1141 acaccgacaa aaacgatttc atcgcccggc cataattccc cgatgcggtg aatcacagtg
     1201 acgcgcccca gcggccagcg gttacgcgct tcatcaacaa tttctgccag tgctttttca
     1261 gtcatccccg gatagtgttc gagggttaat gctttgacgc tgtcgcccag gttatggttg
     1321 cgcaccttac cagtaaaggt gactaccgca ccgtcttcgt cacgctccgc cagccacggg
     1381 tactcttctc ctacgctgaa cggctgcgga ccaacaacaa ttttggtttc tgccatctta
     1441 acctccggtt accggcggga agaaagctac ttcgtcgccg tcagtcagcg gatggtcaaa
     1501 actcaccagc gtctggttga cggcagccag taatttgcca tcttccagcg ccagcgccca
     1561 gcgatcgctc tgcgcagcca tgtgctggcg taacgcttca acagttggga aatccgcagc
     1621 cacttcggtt gcatctgttc ccaccaactc gcgcacctgg gcgaaaaaaa gaactttaat
     1681 catccgcttc caccttaaag tcacccgact tgccgccgct cttcgccagc aaacgtaccg
     1741 gaccaatcac catatctttt tgcaccgctt tgcacatgtc ataaatggtc agcgccgcca
     1801 cggaggccgc ggttaatgct tccatttcga caccggtttt cccggtcagg cggcataagg
     1861 tttctatacg cacccgattg tgctccggct cggcctgtaa attgacttca actttgctga
     1921 gcatcagcgg atgacagagc gggatcagat cccaggtgcg ttttgccgcc tgaataccgg
     1981 caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg accatcaata atcatcgcca
     2041 gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc ttcacgcacg gtttccgctt
     2101 tggcggagac atccaccatg tgcgcttcgc cagcggcgtt gatatgggtc agttgcgaca
     2161 tacttatttc ttcaaatgtg gatggaaatt acacggacgc gtacgggcat ccagctgcgg
     2221 cgcgatgata ttttcccatg cggtacggca cgctttggtc gaacccggca tggcgaaaat
     2281 cagcgttttg ttggcgacgc ccgctaccgc acgagattgc aacgtggaag tgccaatctc
     2341 ttcaaacgac aacatacgga acacttcacc aaaaccttca atttcacggt cgaacaacgg
     2401 cagcaatgct tcgggagcct gatcaccttc cgtcaggcca gtaccacccg taatcaatac
     2461 cacttgtaca tcgtcgctgg cgatccacgc agatacctga gcgcgaatag cgtagcggtt
     2521 ttctttcaca atggctttat cgacaacgtg atggcccgct tcttgcgccg aatcgcgcag
     2581 atagtgaccg gaggtatcgt cttcttcacc gcgacgatta gaaaccgtaa gaatagcaat
     2641 acgggtcggg ataaattcag tgcttacctg actcatctga tctctccttt tgacgtttta
     2701 gccgccaatg tacgataagt tttgcgtaat accggtgttg ttttgatgca ggaaatgggt
     2761 ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc agcgcctgtt gctgggtatc
     2821 gtcttccagc agatcgcgca ggttaacgcc gccttcacca aacaggcaga gatggagttt
     2881 accaatggag gaaacgcgca ggcggttgca agtggcgcag aagtcttttt catacggcat
     2941 gataaggcca atctctccgg cgtaatctgg atggcaaaag acttgcgcgg gaccgtcgct
     3001 gcgttgacgt aattggtgga tccagccgcg acgcagtagc tcgtcacgca gaacctgacc
     3061 agagatgtga tgcttacgga agagctcgct gccctcgccc gtttccatca gttcgatgaa
     3121 acgcagctgg atagggcgat gctggatcca gttcagaaag gtgtcgagct ggtgatgatt
     3181 aacatcacgc atcagcacgg tattgacttt gaccttctca aaaccggcct caaatgcagc
     3241 atcaatcccc gccatgacct ggttgaattt atcctgcccg gtaatagcgt gaaactggcg
     3301 ggcgtccaga ctgtcgacac tgacgttaat gccagtaagt cccgcatcgc gccagttcgc
     3361 cacatcgcgt tccagacggt aaccattggt ggtgaccgca atctggcgga tagcgtcgtt
     3421 ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt aaagacggct caccgcctgt
     3481 caggcgcact ttttcggtgc ccagactggc gaaggcgcgc gtaacccggc gaatttcatc
     3541 gacggtaaga aagcctttat tggtgacgcc gctcggtttg tagccatccg gcaggcagta
     3601 ggtgcaacga aagttacaca catcggtaat cgacaggcgc aagtagtaaa acttacgcgc
     3661 aaatgcatca gtcagttgtg aagccatgta cacctttcca gatacgggag gcgaagtcat
     3721 ttcttccttc gccctggtgg caatctttac gagcattgtc acggccaaag caccgtatca
     3781 gttgacccag gtgcagaggc tagagtgttt agtggttatg ccgatactag cgtgtaaatg
     3841 agatttttac catccacact ttcgctatgt aatcatgtat atagcgtcat gatcgtgcta
     3901 tttcgctaca gaataaggga aaatggcggt tttactttga tatacatcat cagggaggca
     3961 tagcgtgcat cgtcctttag aggtagaaga aaatagtttg tatcctcaat attggcaggt
     4021 taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg ataatctgac tccaggaaac
     4081 tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg ttgctctcgg cggagggcat
     4141 ggactgggac gcgttctctc atcactttcg tctttgggtt ctcgtttaac gggtatcgtc
     4201 accaccaccg ataatggtgg ctcgacgggg cgtattcgcc gttcagaagg cggcattgcc
     4261 tggggcgata tgcgcaactg cctcaaccag ctgataacgg aaccgagcgt cgcctccgcg
     4321 atgtttgaat accgttttgg tggcaatggc gaactttccg gtcataatct cggaaacttg
     4381 atgttaaagg cgctggatca ccttagcgtg cggcctctgg aagccatcaa tttaattcgt
     4441 aatctgctga aagtggatac gcatttgatt ccaatgtcag agcatcctgt tgatctgatg
     4501 gcgattgacg atcaggggca tgaagtttac ggcgaggtca atatcgacca gttaactacg
     4561 ccgattcaag agttattgtt aacgcctaat gtacccgcaa cgcgtgaggc ggttcacgct
     4621 atcaatgaag cggatctcat cattattggg cctggcagtt tttataccag cctgatgcca
     4681 attctgctgc tgaaggaaat cgcccaggca ttacgccgca cgccagcgcc gatggtttat
     4741 atcggcaatc tggggcgtga gttgagttta cctgcggcta atttgaagct ggaaagcaag
     4801 ctggcaatta tggagcagta tgttggtaaa aaggtcattg atgcggtcat cgtcgggcca
     4861 aaagtggatg tctcggcggt gaaagagcgg attgtgatcc aggaggtact ggaggccagc
     4921 gatattccgt atcgtcatga ccgccagttg ttacataacg cgctggaaaa ggcgttacag
     4981 gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc ctattgaaga caaagtgcgc
     5041 gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccgc gcaaattcaa
     5101 tatattgcgg agaaaatgta ggcctgataa gcgtagcgca tcaggctgtt ttccgtttgt
     5161 catcagtctt cttcgctatc ctgttacgat gccgcgataa acagctcacg cagctgatgc
     5221 aactggtcac gaatttgcgc cgcttcttcg aactccagat tctgcgcgtg ttgcatcatc
     5281 aacccttcca gctcatggat tttctgctgc aacgctttag gcgacatatc catcggcaca
     5341 ttatccggct caacaatcgg tcgcgatttt cctctgccct tcgctttggt tttagcaatg
     5401 ttctgcccca acgccaggat atcgaccact ttcttgttca agccttgcgg cgtaatgccg
     5461 tgttcctcgt tgtacttctg ctgtttctcg cggcgacgtt cggtttcgcc aatcgctttc
     5521 gccattgatg gggtgatctt atcgccgtag agaatcgctt taccgttaac gttacgtgcc
     5581 gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc tttgtcagcg
     5641 tcgaggatcg ccaccagcga aacttccggc atatccagac cttcacgcag taagttgatc
     5701 cctaccaata cgtcgaactc acccagacgc aagtcgcgga taatctccat acgctcgacg
     5761 gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt gttcttcgag atattcggta
     5821 agatcttccg ccattcgctt ggtcagtgtg gtgaccagta cgcgttcgtt aattgccgct
     5881 cgctgacgaa tctccgaaag aagatcatca acctgtgtcg ccaccggccg cacttcgata
     5941 atcgggtcaa gcaatccggt tggacgcacc acctgatcca ccacatcgcc gccggatttt
     6001 tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg
     6061 aactcttcaa acttaagcgg acggttatcc agcgctgatg gcaggcggaa gccgtactcc
     6121 accagtgtct ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg tggaatggtg
     6181 acgtgagatt catcgaccac cagcagccca tcagcaggga ggtaatcaaa cagcgtcggc
     6241 ggtggctcac ccggtccacg accggagagg aagcgcgagt agttttcaat ccccgaacag
     6301 tagcccagct cgttcatcat ctccagatca aactgggtac gctgggtcag ccgctgctct
     6361 tccagcagtt tgttgttttc caacagcact ttgcgtctgg cggccagctc ttctttgatc
     6421 tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt agtgcgtttt cgggtagatg
     6481 gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg ggtcaaataa cgacaatcgt
     6541 tccacttcct cgtcaaacag ttccacgcga agtgcaatgt catccgattc tgccgggaag
     6601 atatctatca cctcgccacg aacgcggaaa gtaccacgct ggaatgcttg atcattacga
     6661 gcgtattgca gctccgccag tcggcgcaga atcgcgcgct gatcgataat cataccgacc
     6721 gtgagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata aatcgcggaa
     6781 acagacgcca ccacaaccac atcacgccgc tccagcatcg ctttggtggc ggacaaacgc
     6841 atctgctcaa tatgttcgtt aaccgaggca tctttctcaa tgaaagtgtc ggaactcggt
     6901 acataggctt ccggctgata gtagtcgtag taggaaacga aatattccac cgcgttttcc
     6961 gggaagaact ctttcatttc gccatacagc tgggccgcca gcgttttgtt gggcgcaagt
     7021 accatggttg ggcgctgaag gtcagcaatg acattggcaa tggtgaatgt tttcccggag
     7081 ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt ccagcccctc ttcgagacgt
     7141 cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg aattcagttt gaacggttta
     7201 ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac tcgtcgtact
     7261 taattatgcc aacaaattat actggataaa aaaacagttc atcaccataa tatttctgtt
     7321 acagcgtaaa ctccgctgtc aatcatgagc aaaatttact ctgtggcgag ataaaactcc
     7381 ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag atgagtgatg
     7441 acagttttat ggcaagagat gcctgttcag tgactgccat tgcatttatc taaccagtta
     7501 aaaaataaaa gatatttctt tgagccgtct ttaacgccaa ttcgcgtgca agccgcgtat
     7561 tctctcgctt gcctcgtgtt ttctaactct aatacacatg gttatccaca ggaatagtgg
     7621 ataactgtct ccagccccta tccccc
//
LOCUS       DAZKGS010000195         7591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKGS010000195 DAZKGS010000000
VERSION     DAZKGS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            171..1568
                     /gene="ulaA"
                     /locus_tag="ACHOQY_004275"
     CDS             171..1568
                     /gene="ulaA"
                     /locus_tag="ACHOQY_004275"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9680248.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1584..1889
                     /gene="ulaB"
                     /locus_tag="ACHOQY_004276"
     CDS             1584..1889
                     /gene="ulaB"
                     /locus_tag="ACHOQY_004276"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9680249.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1899..2363
                     /gene="ulaC"
                     /locus_tag="ACHOQY_004277"
     CDS             1899..2363
                     /gene="ulaC"
                     /locus_tag="ACHOQY_004277"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9680250.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2377..3027
                     /gene="ulaD"
                     /locus_tag="ACHOQY_004278"
     CDS             2377..3027
                     /gene="ulaD"
                     /locus_tag="ACHOQY_004278"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9680251.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3037..3891
                     /gene="ulaE"
                     /locus_tag="ACHOQY_004279"
     CDS             3037..3891
                     /gene="ulaE"
                     /locus_tag="ACHOQY_004279"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9680252.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3891..4577
                     /gene="ulaF"
                     /locus_tag="ACHOQY_004280"
     CDS             3891..4577
                     /gene="ulaF"
                     /locus_tag="ACHOQY_004280"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9680253.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4706..4981)
                     /gene="yjfY"
                     /locus_tag="ACHOQY_004281"
     CDS             complement(4706..4981)
                     /gene="yjfY"
                     /locus_tag="ACHOQY_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9680254.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5308..5703
                     /gene="rpsF"
                     /locus_tag="ACHOQY_004282"
     CDS             5308..5703
                     /gene="rpsF"
                     /locus_tag="ACHOQY_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9680255.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5710..6024
                     /gene="priB"
                     /locus_tag="ACHOQY_004283"
     CDS             5710..6024
                     /gene="priB"
                     /locus_tag="ACHOQY_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9680256.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6029..6256
                     /gene="rpsR"
                     /locus_tag="ACHOQY_004284"
     CDS             6029..6256
                     /gene="rpsR"
                     /locus_tag="ACHOQY_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9680257.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6298..6747
                     /gene="rplI"
                     /locus_tag="ACHOQY_004285"
     CDS             6298..6747
                     /gene="rplI"
                     /locus_tag="ACHOQY_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9680258.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6818..>7591)
                     /locus_tag="ACHOQY_004286"
     CDS             complement(6818..>7591)
                     /locus_tag="ACHOQY_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9680259.1"
                     /translation="YSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDHNCDGRRT
                     VEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKYNKAHAGA
                     RLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWS
                     SELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGL
                     GFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1816 a         1837 c         2071 g         1867 t
ORIGIN      
        1 aaataattgt ttcgttgtgt gaatcccact ctatccgtgt ggaattattt gcgggtcgcg
       61 tcacatttaa tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca
      121 acattccggg tgtgcggaat acccggttac ctcttcttca ggagatcgtt atggagatcc
      181 tctacaacat ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg
      241 gtattgtgac ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag
      301 gcacgattaa aaccataatt ggtttcatgt tgttgcaggc agggtccggc atcctcacca
      361 gcaccttcaa accggtggtg gcgaaaatgt ccgaagtcta cggcattaac ggcgcaattt
      421 ccgatactta cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg
      481 taggttacgc ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca
      541 ttaccggtat tcgtaccatc atgctgactg gttatatcat gttccagcag gcggggctga
      601 ttgccgttac gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga
      661 ttctggtttc gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag
      721 tgacggacgg ctgcggtttc tccatcggtc accagcaaca gtttgcatca tggattgcct
      781 ataaagttgc gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag
      841 gctggctgaa tattttccac gacaacatcg tctccacggc gattgtgatg accatcttct
      901 ttggtgccat tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtga
      961 actggacggt gtacatcctg caaactggtt tctccttcgc ggtggcgatc ttcatcatca
     1021 ctcagggcgt gcgcatgttt gtggcggaac tctctgaagc atttaacggt atctcccagc
     1081 gcctgatccc tggtgcggtt ctggcgattg actgtgcggc tatctatagc ttcgcgccga
     1141 acgccgtggt ttggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc
     1201 tggtcgcctg cggttcctcg atcctgatta ttcccggctt tatcccgatg ttcttctcca
     1261 acgccaccat cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagatat
     1321 gtctggtgat ggggatgatt gaaatctttg gctgcgtctg ggcggtgaaa ctcaccggta
     1381 tgagcgcctg gatgggcatg gcggactggt cgattctggc accgccgatg atgcagggct
     1441 tcttctccat cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt
     1501 tcttcgctgg ccgcgcactg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt
     1561 ctgcttaata aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac
     1621 aaggcagttc catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg
     1681 accacacggt aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata
     1741 tcatcatcgc ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg
     1801 ttggcgtgcg caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca
     1861 aagcgcattt cccgcaggat gtgaagtaag gacgcgccat gaaattacgt gattcgctgg
     1921 cggagaataa atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa
     1981 tcggcgttga cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc
     2041 tggatgcggt tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc
     2101 acggtcgccc ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc
     2161 cgctggagtt caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg
     2221 tcgatgccaa cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg
     2281 aagaaaattt cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg
     2341 accgcaccaa cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc
     2401 gcgctggaca accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa
     2461 gtcgacatta tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgt
     2521 gacctgaaag cgctctaccc gcacaaaatc gtactggcag acgctaaaat tgctgatgcg
     2581 ggcaaaatcc tttcgcgtat gtgcttcgaa gccaacgctg actgggtgac ggtaatttgc
     2641 tgtgcggata tcaacaccgc caaaggcgcg ctggacgtag caaaagagtt taacggcgac
     2701 gtgcagatcg aattgaccgg ctactggacc tgggaacagg cgcagcagtg gcgtgacgca
     2761 ggcattcagc aggtggttta tcaccgcagc cgtgacgcgc aggccgcagg cgtagcgtgg
     2821 ggcgaagcgg acattaccgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc
     2881 accggtggcc tggcgctgga agatctgccg ctgttcaaag gtattccgat tcatgtgttt
     2941 atcgctggtc gcagcatccg tgatgccgct tctccggttg aagctgcacg tcagttcaaa
     3001 cgttccatcg ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt
     3061 ggcatctatg aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcaactggca
     3121 aaaacgttgg gcttcgattt tgtcgagatg tcggtagatg aaacggacga gcgcctgtcg
     3181 cgcctcgact ggagccgcga gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc
     3241 gtgcgcgtgc cgtccatgtg cctttctgct catcgtcgtt tcccgctggg cagtgaagat
     3301 gacgcggtgc gggcgcaggg gctggagatt atgcgtaaag ctatccagtt cgcccaggac
     3361 gtcggcattc gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac
     3421 gaaacgcgtc gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg
     3481 caggtgacgc tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg
     3541 ctgggttacg cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac
     3601 ctgtcggcgt gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc
     3661 gcggtacatg tgaaagacac caaacctggc gtcttcaaaa acgtgccgtt tggcgaaggt
     3721 gtagtggatt tcgaacgttg tttcgaaacg ctcaaacaga gtggctattg cgggccgtac
     3781 ctgattgaga tgtggagcga aacggcggaa gacccggcgg cagaagtggc gaaagcgcgt
     3841 gattgggtga aagcgcgcat ggcgaaagcg ggcatggtgg aggcggcata atgcaaaagc
     3901 taaaacagca ggtatttgaa gccaacatgg atctgccgcg ctacgggctg gtgaccttta
     3961 cctggggcaa cgtcagcgct atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg
     4021 gcgtcgccta cgaaaccatg aaagcagacg atatggtggt ggtcgatatg agcggtaagg
     4081 tggtggaagg ggagtatcgc ccatcttccg acaccgcgac gcatcttgaa ctctaccgtc
     4141 gttatccgtc gcttggcggc attgtccata cccactccac tcatgctacc gcatgggcgc
     4201 aggcggggct ggcgatcccg gcgttaggca ccacgcacgc tgactacttc tttggtgaca
     4261 tcccgtgtac gcgtggatta agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg
     4321 gcaaagtgat tatcgaaacg ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg
     4381 tgtatcagca cggaccgttc gcctggggga aagatgccca cgacgcggtg cataacgcgg
     4441 tagtgatgga agaagtggcg aaaatggcgt ggattgcgcg cagtattaat ccacaactca
     4501 atcacatcga cagcttcctg atgaataaac acttcatgcg taagcatggt cctaacgctt
     4561 attacgggca gaagtaaaac acgcgctgcg gaaatttcct tcttcgggag ataactggtc
     4621 taattccgca gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc
     4681 aatgtttcgt tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc
     4741 cgttactccc cggttcccgc atcagaatta cgtggtagta cgttgcgccc tgcgcatctg
     4801 tttcttcatt taatgcctga cgtgcttcac tttcagtggc gaaattatga ttgatataaa
     4861 tcacgcctaa gctttgcaca tcgtccatat ttctggcctg gtggttattg atttcaatgg
     4921 ctgcccatgt atttgcactt agcaaaagca cagccagaag ggctaaaaca cgactgaaca
     4981 tagatacctc ctcaacggct gactttgtgt gctctcctct gtgatgatct tctgatttaa
     5041 ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagttgt tcgaaaaaac
     5101 actgtgacgg aatgtgatga acttcaaatc agggagttag ttcttttgag cgaaagatga
     5161 gatttatttg tcggctggcc ttgagtttag cggggcatac aagtactata acgcgtcatt
     5221 tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag
     5281 gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat
     5341 cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc catcactggt
     5401 gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc
     5461 aacaaactgc acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc
     5521 gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt
     5581 accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag
     5641 cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag
     5701 taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga
     5761 aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag
     5821 gaggaagccg gctttcaccg gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac
     5881 gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc
     5941 atttcatgcc acaaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt
     6001 gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg
     6061 ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa
     6121 ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata
     6181 ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac
     6241 tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg
     6301 caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt
     6361 aaagcgggct atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag
     6421 aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact ggctgaagtt
     6481 ctggcagctg ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg
     6541 tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac
     6601 gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt
     6661 ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa
     6721 gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc
     6781 attggtcggc gttttgcttt ctatttttcg tcaggtatta gtttcgcaag tagatccatt
     6841 caccattctc ttgtgtccat aagtcaggat ggtttgctgg cgttagatgc atacgatcac
     6901 tccttatgtc tcggtcctgc ggcactcgct cgaaacctaa acccgagtag attgctgctg
     6961 aatttgataa ggaacgtaga ccaattttca aatcatgggg taaatcagaa tacgcgttta
     7021 gcaatgcagc aatgatgaat ccgagggcat tagaaacgcc tgtaacagtg cgaagttggt
     7081 cgatataaag atcgattgtt ggtagttcag atgaccagca attaggcatt tgggcaggaa
     7141 gggttacaac gtatcctatc gtttggccgg tatagtctgg agtaccataa ccaggtccca
     7201 aaaccacttc gtgtattgtg tatttactgt tgggttcgaa ggattcgaca tcttgcaata
     7261 aatatttcag ttctgcggtt aacctcgcac ccgcgtgagc tttattgtat ttctgggcgt
     7321 gaatattatc taatgtagct ttatggttcc atccaaaaga attatcacca ttcattgtta
     7381 ctccatcgta agcattacac ttcgttgcta acttattgaa tgcttgccag tcagagagat
     7441 ctagtttgtg gatctcaaca gtacgtctgc catcgcaatt atgatctctt ccacgatttg
     7501 gcagtaatgt agcacgtgca tattgatgaa attttaattt tactggtttg tcggtggacg
     7561 aaaaatgggg tgaatatgca ggaaatgaat a
//
LOCUS       DAZKGS010000196         7535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKGS010000196 DAZKGS010000000
VERSION     DAZKGS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..656
                     /gene="ecpR"
                     /locus_tag="ACHOQY_004287"
                     /gene_synonym="matA"
     CDS             114..656
                     /gene="ecpR"
                     /locus_tag="ACHOQY_004287"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9680260.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            730..1317
                     /gene="ecpA"
                     /locus_tag="ACHOQY_004288"
                     /gene_synonym="matB"
     CDS             730..1317
                     /gene="ecpA"
                     /locus_tag="ACHOQY_004288"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9680261.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1375..2043
                     /gene="ecpB"
                     /locus_tag="ACHOQY_004289"
                     /gene_synonym="yagY"
     CDS             1375..2043
                     /gene="ecpB"
                     /locus_tag="ACHOQY_004289"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9680262.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2069..4594
                     /gene="ecpC"
                     /locus_tag="ACHOQY_004290"
                     /gene_synonym="yagX"
     CDS             2069..4594
                     /gene="ecpC"
                     /locus_tag="ACHOQY_004290"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9680263.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     STTLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4584..6227
                     /gene="ecpD"
                     /locus_tag="ACHOQY_004291"
     CDS             4584..6227
                     /gene="ecpD"
                     /locus_tag="ACHOQY_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP9680264.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6196..6906
                     /gene="ecpE"
                     /locus_tag="ACHOQY_004292"
     CDS             6196..6906
                     /gene="ecpE"
                     /locus_tag="ACHOQY_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9680265.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7219..>7535
                     /gene="ykgJ"
                     /locus_tag="ACHOQY_004293"
     CDS             7219..>7535
                     /gene="ykgJ"
                     /locus_tag="ACHOQY_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9680266.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGL"
BASE COUNT         1948 a         1933 c         1968 g         1686 t
ORIGIN      
        1 cttatttact tccggtttac taacgaaact gaatgcacct gtaaaaatta caggtttgga
       61 aagtagtgac atggcaaagt gattacagca gggactatga ggttaaaaac catatggaat
      121 gtcaaaaccg ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac
      181 tatctgaact gattgtggat atcgacagat taatttatct atcgttggag aaaattagaa
      241 aagatttcgt gtttatcaat ctcaatacgg attctttatc tgaatttata aaccgtgata
      301 atgaatggtt atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag
      361 aagccttagc aaattattgg tattacaaca gcaatattag gggcgtggta tacgcaggac
      421 tgagtcgtga tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag
      481 atattaagaa agataaaatc acggaccggg aaatggaaat tatccgcatg acggcccagg
      541 gaatgcaacc taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc
      601 atcggcgtaa tgctgaggcc aagctgtact caaaaatata taagttggtt cagtaaactc
      661 caggctagtt agttttaaaa atgactcact gggacatcac gtcctcaatt caactcggga
      721 agaaatgcaa tgaaaaaaaa ggttctggca atagctctgg taacggtgtt taccggtata
      781 ggtgtggcgc aggctgctga cgtaacagct caggctgtag cgacctggtc agcaacagcc
      841 aaaaaagaca ccaccagtaa gctggttgtg acgccactcg gtagcctagc gttccagtat
      901 gccgaaggca ttaaaggttt taactcacag aaaggtctat ttgacgtggc tatcgagggt
      961 gactcaacgg ctaccgcctt taaactgacc tcacgtctta tcaccaacac attaacccag
     1021 ttggatacct caggttccac actgaatgtg ggcgtggatt ataacggcgc ggcagtcgaa
     1081 aaaactggcg ataccgtgat gatcgatacc gccaacggcg tactgggcgg caaccttagc
     1141 ccgctggcaa acggttacaa tgccagcaat cgtaccaccg cacaggatgg tttcactttc
     1201 tccatcatca gcggcaccac caatggtacc accgcagtaa ccgattacag cactctaccg
     1261 gaaggcatct ggagcggcga cgttagcgta cagttcgacg cgacctggac cagttaatct
     1321 ctctgatgta ccagcagggg tagcccccct gctttattcc tggacggaat tgttatgaaa
     1381 aagcaccttc tgcctctcgc tctgctgttt tccggaatat ctccggccca ggcgctggat
     1441 gtcggcgata tatcatcgtt tatgaacagt gacagcagca cgctgagcaa aacgatcaaa
     1501 aacagtaccg acagtggtcg ccttatcaat atccgtctcg aacggctctc ttcaccgctt
     1561 gacgacgggc aggttatctc aatggacaag ccggatgagt tgctactcac tcccgccagc
     1621 ttgctgctac ccgcccaagc cagcgaagtg atccgcttct tctataaggg acccgcagat
     1681 gaaaaagagc gctactaccg cattgtctgg tttgatcagg ccctcagtga tgcgcagcgc
     1741 gataatgcca accgcagcgc tgtggccact gcttccgccc gcatcggcac cattctggtc
     1801 gtcgcccctc gtcaggcgaa ctaccacttt cagtacgcca acggctccct gacaaataca
     1861 ggaaatgcga cgctgcggat cctcgcttac ggcccctgcc tgaaagccgc caatggtaag
     1921 gagtgtaaag agaattacta cctgatgcca ggcaagtcgc gtcgttttac ccgcgtggac
     1981 acggcggata acaaaggacg ggttgcactt tggcagggtg ataagttcat tcccgtgaaa
     2041 tagatagctg tgcagatgga taacgacaat gcctttacga cggttctccc caggactgaa
     2101 agcccagttt gccttcggca tggtcttttt gttcgttcaa cccgatgcca gcgctgctga
     2161 cataagtgcg cagcaaatag gtggggtgat tattccgcag gccttcagtc aggcgcttca
     2221 ggacggcatg agcgtcccgc tctatattca tctcgccggt agccagggtc gccaggacga
     2281 tcagcgaatc ggcagcgctt ttatctggct ggatgatgga cagctacgca tccggaaaat
     2341 acagctggaa gagagtgaag ataacgccag tgtcagcgaa caaactcgac agcagctgat
     2401 ggctctggcg aacgccccgt tcaatgaggc ccttaccatc cccctgactg acaacgcgca
     2461 gctggatctc agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg
     2521 caccgtacta cgctcacgta gcgaagacat cgggcagtcc agtgttaaca ccctcagcag
     2581 taatctgagc tataacttgg gcgtctataa caaccagttg cgtaacggcg ggagcaacac
     2641 atccagctat ctgtcgctga ataacgttac tgcgctgcgc gaacatcatg tggtgctcga
     2701 cggctcgctg tacgggatcg gtagcggtca acaggacagt gaattatata aagcgatgta
     2761 tgaacgcgat tttgccggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca
     2821 gtccttaggg ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa
     2881 ccaggccagc tccaccatct tcgacagcag ccagtcagcc acgccagtga tcgccttttt
     2941 accggcggcg ggcgaagtac atctcacccg tgatgggcgg ctactaagcg ttcagaactt
     3001 cactatgggc aatcatgaag tggatacccg gggtctaccg tacgggattt acgatgtgga
     3061 agttgaggtg atcgttaacg gtcgcgtgat cagcaaacgc ccccagcggg tcaataagct
     3121 gtttagccgg gggcgcggcg tcggtgcacc actggcgtgg caggtatggg gcggtagctt
     3181 tcatatggat cgctggtcgg aaaacgggaa aaagacgcga ccagctaaag agagttggct
     3241 agcaggtgcc tcgacctccg gctcattgag tacgcttagc tgggcggcaa cgggatatgg
     3301 atacgataat caggcggtgg gtgaaacccg tctgacgctg ccgcttgggg gggcgatcaa
     3361 cgttaacctg caaaatatgc tggccagtga cagctcatgg agcagcatcg gcagcatcag
     3421 caccactcta ccgggaggct ttagttcgct gtgggttaac caggaaaaaa cccgcattgg
     3481 caatcaattg cgacgtagcg atgccgacaa ccgtgcaatc ggcggcacac tcaacctgaa
     3541 ctcactgtgg tcgaagctgg gcacattcag catcagctac aatgatgacc gccgttacaa
     3601 cagccattat tacacggcag attactatca aaatgtctac agcggtacct ttggttcgct
     3661 tggcctgcgg gccggtattc agcgctataa caacggcgac agcaacgcca atacagggaa
     3721 atatatcgct ctcgatctct cgctaccact gggcaactgg tttagcgcag ggatgactca
     3781 tcaaaacggc tacaccatgg caaacctgtc agcacgcaaa cagtttgatg aaggaaccat
     3841 tcgcactgtt ggtgccaatc tgtcacgtgc catctccggc gataccggtg atgacaaaac
     3901 cctcagcggt ggggcgtatg cacagttcga cgctcgttac gccagcggaa cgctgaacgt
     3961 caatagcgcg gcggacggct acatcaatac caacttgacc gccaatggca gcgtcggctg
     4021 gcagggtaaa aacatcgctg ccagcgggcg gactgatggc aacgctgggg tgatattcaa
     4081 caccgggctg gaggacgacg gtcagatcag cgccaaaatc aacgggcgga ttttcccgct
     4141 taacggcaag cgtaactatc tcccgctctc tccctatgga agatatgagg tggagttaca
     4201 gaacagcaaa aactcactcg acagctacga tatcgtcagc ggccgcaaaa gccatctgac
     4261 tctctatcca ggcaatgtcg ctgtcattga gccagaggtg aagcagatgg ttaccgtctc
     4321 cggtcgtatc cgtgcggaag acggcacact gctggctaac gcacggatta acaaccatat
     4381 cggccgaacc cgaaccgatg aaaacggcga gtttgtcatg gacgtggata agaaatatcc
     4441 cactatcgat tttcgctaca gtggcaataa aacctgcgaa gtggcactgg aactcaacca
     4501 ggcgcgcggt gccgtctggg tcggtgatgt ggtctgcagc ggcctctcat cgtgggcgga
     4561 ggtgacgcag acaggagaag agaatgagag ttaacctact aataacgatg ataattttcg
     4621 cgctaatctg gccagtaact gagctcagag cggcagtgag caaaacaacc tgggcggatg
     4681 caccggcacg cgagtttgtg tttgtcgaaa acaactcaga cgacaacttt ttcgtcactc
     4741 ctggcggggc gctggatccg cgcctgaccg gtgccaaccg ctggaccggt ttaaaataca
     4801 ctggttcagg aaccatctat cagcaaagcc tcggctacat tgataacggt tacaacaccg
     4861 gcctttatac caactggaag tttgatatgt ggctggaaaa ttcaccagtt tcatctcctt
     4921 taactggctt gcgctgcatc aactggtacg ctgggtgtaa tatgaccacc agtcttatcc
     4981 tgccgcaaac caccgacgcc agtggatttt atggcgcgac ggtgaccagc ggcggcgcga
     5041 agtggatgca cggcatgttg tcagacgcgt tttaccagta tctgcaacaa atgcccgtcg
     5101 gcagcagctt tacaatgacc atcaatgcct gccagacctc tgtgaactat gacgccagca
     5161 gcggcgcacg ctgtaaggat caggcctccg gcaactggta tgttcgcaac gtcacccata
     5221 cgaaagcagc aaatctgcgg ttaataaata cccactcgct ggcggaggta tttatcaaca
     5281 gcgacggagt accgactctg ggcgaaggga acgccgactg ccggacacag accatcggca
     5341 gcctttcagg attaagttgt aagatggtca actataccct gcaaacaaac ggactcagca
     5401 acacctcaat ccatatattc ccggcgatcg ccaactcgtc gttagcctcg gccgtcgggg
     5461 cgtacgatat gcagttcagt ctgaatggca gttcatggaa accggtgagc aataccgcct
     5521 attactacac cttcaacgag atgaagagcg cagactcgat ctatgttttc ttctcgagca
     5581 acttctttaa gcagatggtg aacctcggga tcagcgatat caacaccaaa gatctattca
     5641 actttcgctt tcagaacacc acatcaccgg agtctggctg gtatgaattt tctacctcca
     5701 acacgctgat tatcaaaccc cgtgatttca gcatcagtat tatctccgat gaatatactc
     5761 agacaccgtc gcgggaggga tatgttggca gcggcgagtc ggcactcgat ttcggctata
     5821 tcgtaaccac cagcggtaaa acagctgccg acgaagtgct gatcaaggtg accggacccg
     5881 cgcaggtgat tggcgggcgc tcctattgtg tcttcagctc cgatgacggt aaggcgaaag
     5941 taccgttccc ggcgacactt tcctttatta cccgcaacgg agctacaaaa acctacgatg
     6001 ccgggtgcga tgatagctgg cgggatatga ccgatgcgct gtggttgacc acaccgtgga
     6061 ctgatatctc tggcgaagtg gggcagatgg ataagaccac agtcaaattt tcgattccaa
     6121 tggataacgc catttcgctg cgtacggtag atgataacgg ctggtttggc gaagtcagcg
     6181 cttcaggaga gattcatgtt caggcgacgt ggcgtaacat taactaaggc cctgctgaca
     6241 gcggtctgta tgctggcggc acctttgaca caggcgattt cggtcggcaa tctgacattt
     6301 tcgctgccgt ccgagactga ctttgtcagc aaacgtgtag tgaataacaa caaaagcgca
     6361 cggatatacc gtattgccat cagtgctatt gatagcccgg gcagcagtga attgcgcacc
     6421 cgaccggtgg atggtgaact gctttttgcc ccccgccaac tggcgttgca ggctggtgag
     6481 agcgagtatt ttaaatttta ctatcatggt ccacgggata accgcgagcg ctactaccgg
     6541 gtctcatttc gcgaagtccc cactcgtaac cagacaagac gtagcccaac cggcggcgag
     6601 gtcagcacgg agccggtggt ggtgatggat accattctgg tagtacgacc acgtcaggtt
     6661 cagtttaaat ggtccttcga caaggtgaca ggaacggtaa gtaacactgg caacacatgg
     6721 tttaagctac tgattaagcc aggatgtgat tcgaccgaag aggaaggcga tgcctggtat
     6781 ctacgtccgg gagacgtggt tcatcagcct gagttacgtc agccggggaa tcattatctg
     6841 gtctataacg acaaattcat taagattagc gattcctgtc cggctaagcc cccttcggcg
     6901 gactaagact tatccatcgg tcgagggaag aattccacct cagagctcca aaaatcgttt
     6961 aaatgatgtg gaagcgatcg tcaatgtcac ctgtaacgat gggctttctc atatgcacac
     7021 catagtgttc aggagacacg gaaaggtatc aggcagcctg ctaatgagtg gtttaatcac
     7081 gaattagtac gtaaaatcgg taacggctgg aaatcattca atactcacac tatcgaaagt
     7141 tccccagcca accgcggtac gttcttacat acgatgtacc gctgttctct ttacgatttt
     7201 tagctgtact ggtgaattat gagcaatctg aatccatgca tgacgtgtgg tgcctgttgt
     7261 gcatttttcc gcgtctcttt ttactgggcc gaagccgacg atgctggcgg tactattccc
     7321 gccaggctca ctgaacaaat atcccctttt caccgatgca tgagcggtac caatcagaaa
     7381 aacccccgat gtattgccct tgcaggaacc ccgggcaaaa atgcctgctg cacgatatat
     7441 aaaaatcgat cgtccacatg cagagaattt gccatgtctg gtgagaacgg agtcgtcaat
     7501 gaggcttgca atcgtgcaag ggctaaatac gggct
//
LOCUS       DAZKGS010000197         7525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKGS010000197 DAZKGS010000000
VERSION     DAZKGS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..915)
                     /locus_tag="ACHOQY_004294"
                     /pseudo
     CDS             complement(92..915)
                     /locus_tag="ACHOQY_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(969..2687)
                     /gene="proS"
                     /locus_tag="ACHOQY_004295"
     CDS             complement(969..2687)
                     /gene="proS"
                     /locus_tag="ACHOQY_004295"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9680267.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="ACHOQY_004296"
     CDS             complement(2799..3506)
                     /gene="tsaA"
                     /locus_tag="ACHOQY_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9680268.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="ACHOQY_004297"
     CDS             complement(3503..3907)
                     /gene="rcsF"
                     /locus_tag="ACHOQY_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9680269.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="ACHOQY_004298"
     CDS             complement(4025..4840)
                     /gene="metQ"
                     /locus_tag="ACHOQY_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9680270.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4880..5533)
                     /locus_tag="ACHOQY_004299"
     CDS             complement(4880..5533)
                     /locus_tag="ACHOQY_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9680271.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5526..6557)
                     /gene="metN"
                     /locus_tag="ACHOQY_004300"
     CDS             complement(5526..6557)
                     /gene="metN"
                     /locus_tag="ACHOQY_004300"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9680272.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6745..7320
                     /gene="gmhB"
                     /locus_tag="ACHOQY_004301"
     CDS             6745..7320
                     /gene="gmhB"
                     /locus_tag="ACHOQY_004301"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9680273.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1850 a         1946 c         1909 g         1820 t
ORIGIN      
        1 aatctctgca atctattgaa tttgcatgtt tttgtaggcc ggataaggcg tttacgccgc
       61 atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg
      121 ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa
      181 acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt
      241 gcgccagtgt aagtggatgc ccggctttgg caaaggcatc cgtgacgaat tccgaacaaa
      301 accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga
      361 cgcactttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
      421 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
      481 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
      541 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
      601 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
      661 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
      721 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
      781 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
      841 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaaacaggc ggcagtacgc
      901 ctttggttta tccattttat acaatccatg taaaaaaagg gccctgaaat tcaggaccct
      961 ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt
     1021 aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg
     1081 tcgcccagca caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc
     1141 ggacgctctt tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac
     1201 agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc
     1261 acctggaacg gcgcgatagc gtcaggccat acgatgcctc gttcgtcgta gttctgctca
     1321 atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg
     1381 ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc
     1441 tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg
     1501 cttggatcgc cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga
     1561 tcccagttga tgccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc
     1621 atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa
     1681 cccggaccgg ctttaaccac ggcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt
     1741 gcaacctgcg gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc
     1801 agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt
     1861 ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta
     1921 tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct
     1981 gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg
     2041 ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggct
     2101 tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt
     2161 gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc
     2221 aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc
     2281 tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg
     2341 tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca
     2401 aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcagct
     2461 ggctgaacca ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg
     2521 atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag
     2581 gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg
     2641 gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc
     2701 cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaaagtg atttagttta
     2761 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
     2821 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
     2881 taggttttgc ccgtttcctc acctttacga taggccgggc gtgggtcctg cgccagtact
     2941 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
     3001 acctctgcgg taaaactcac cgccatctct gccgctggcg cactttgcgc atagctggca
     3061 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
     3121 ccatcgacca gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc
     3181 tctttcagct ctaccagcga cataccaatt gggttagggc ggaaagtaga gcgtgttgcg
     3241 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
     3301 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaacgc ttccaggccg
     3361 cgaacggcgt cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt
     3421 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
     3481 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
     3541 cagcctgacg atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaataccg
     3601 cattggcctt cattttcgag gcgttgattt gcatccgctt acgcgcggtt ggaatgctcg
     3661 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
     3721 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
     3781 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
     3841 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
     3901 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
     3961 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
     4021 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
     4081 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
     4141 gcgttacgat caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg
     4201 ctggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
     4261 cgtcgtccag agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg
     4321 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
     4381 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
     4441 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
     4501 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
     4561 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
     4621 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
     4681 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
     4741 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
     4801 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
     4861 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga
     4921 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
     4981 tatagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
     5041 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
     5101 ccggcaatgc ttccggtaac agcactttac ggacgatctg catcggcgtg gcacccattg
     5161 cacgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
     5221 caataaacgg tgctgcacca acggttaacg gaacaatcgc tgcctgcaaa ccaatcgatg
     5281 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
     5341 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
     5401 gccccgggcg cgtaacataa agcaggacgc cgaccggcag accaatcaca aagccaaaaa
     5461 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc aaccacatca
     5521 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
     5581 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttaacg
     5641 ccaccggcgt aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
     5701 gtttcagaaa gcagcggggc atcgaccgat tgaccggtaa actccaggcg caacatcggc
     5761 acgcagtcag taaatggctc cgcttgcaga cgttcctggt aatcttccgg gatatccaga
     5821 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
     5881 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
     5941 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
     6001 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
     6061 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
     6121 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
     6181 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
     6241 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
     6301 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg
     6361 ccatcgacca gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc
     6421 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
     6481 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
     6541 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatctcgaa
     6601 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
     6661 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
     6721 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
     6781 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
     6841 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
     6901 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
     6961 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
     7021 ccgcatcatc cgcagggtag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
     7081 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
     7141 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
     7201 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
     7261 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcacaatga
     7321 ttaaaagatg agcggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
     7381 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
     7441 ggttgaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata
     7501 cgccacctcg cgacagtgcg ctaaa
//
LOCUS       DAZKGS010000198         7490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKGS010000198 DAZKGS010000000
VERSION     DAZKGS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..187
                     /locus_tag="ACHOQY_004302"
     CDS             <1..187
                     /locus_tag="ACHOQY_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680274.1"
                     /translation="CEKAAESLHHAERNCTQQTDTSLSDEGGERLRPGRIAVPDNSVL
                     YLPVSTFVGTGFFIHPL"
     gene            198..3047
                     /gene="cah"
                     /locus_tag="ACHOQY_004303"
     CDS             198..3047
                     /gene="cah"
                     /locus_tag="ACHOQY_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFP9680275.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            3158..5543
                     /locus_tag="ACHOQY_004304"
                     /pseudo
     CDS             3158..5543
                     /locus_tag="ACHOQY_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            5540..6445
                     /locus_tag="ACHOQY_004305"
     CDS             5540..6445
                     /locus_tag="ACHOQY_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9680276.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            6442..>7490
                     /locus_tag="ACHOQY_004306"
     CDS             6442..>7490
                     /locus_tag="ACHOQY_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9680277.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALR"
BASE COUNT         1870 a         1869 c         2207 g         1544 t
ORIGIN      
        1 ctgcgaaaag gcagctgaat ctcttcatca tgcagaacgg aattgcacac aacagactga
       61 tacttctctg tctgatgaag ggggagagcg tttacgcccg ggacgtatcg ctgtgcccga
      121 taactctgtt ttgtacctgc cggtatccac ttttgtgggt accggctttt ttattcaccc
      181 tctgtaagga aaagctgatg aaacgacatc tgaacaccag ctacaggctg gtatggaatc
      241 acattacggg caccctggtg gtggcctccg aactggcccg ctcacgggga aaacgcgccg
      301 gtgtggcggt tgcgctgtct cttgctgctg tcacatcagt cccggcactg gctgctgaca
      361 aggttgtaca ggcgggagaa accgtgaacg atggaacact gacaaatcat gacaaccaga
      421 ttgtcttcgg tacggccaac ggaatgacca tcagtaccgg gctggaactg gggccggaca
      481 gtgaagaaaa caccggtggg caatggatac agaatggcgg gatagccgga aacaccactg
      541 tcaccacaaa tggtcgtcag gtcgtgctgg aggggggaac agccagtgat acggttattc
      601 gtgacggcgg gggacagagc ctgaacggac tggcggtgaa caccacactg aataacagag
      661 gcgagcagtg ggtgcatgag ggcggggttg ccaccggtac aattatcaac cgcgacggtt
      721 accagagcgt taaaagtggc gggctggcaa caggaaccat catcaacacc ggcgcagaag
      781 gcggccctga ttctgacaac tcgtatacgg gtcagaaggt ccagggaaca gcagaatcca
      841 ccaccatcaa caaaaatgga cggcagatta tcttattttc cgggctagcc cgtgacactc
      901 tcatttacgc aggtggtgac cagtcggtac acggaagggc cctgaatacc acactgaatg
      961 gcggttacca gtatgtgcac agggacggac ttgcgctgaa cacggtaatt aacgaggggg
     1021 gctggcaggt tgttaaggca ggtggcgctg ccggtaacac caccataaat cagaacggtg
     1081 aactgagggt acatgccggc ggggaagcca ctgcagtcac ccagaacacg ggcggtgcac
     1141 tggttaccag tactgctgca actgtcatcg gcacaaaccg tctggggaat ttcacggtgg
     1201 aaaacggtaa ggctgacggt gttgttctgg aatccggcgg tcgtctggat gtactggaga
     1261 gccattcagc acagaatacc ctagtggatg acggcggtac cctggcagtg tctgccggcg
     1321 gtaaggcgac aagtgtcacc ataacatccg gtggtgccct gattgcagac agtggtgcca
     1381 ctgttgaggg gaccaatgcc agcggtaagt tcagtattga tggcacatcc ggtcaggcca
     1441 gcggcctgct gctggaaaat ggcggcagct ttacggttaa tgccggggga caggctggca
     1501 acaccactgt cggacatcgt ggaacactga cgctggctgc cgggggaagt ctgagtggca
     1561 gaacacagct cagtaaaggc gccagtatgg tactgaatgg tgatgtggtc agtaccggcg
     1621 atattgttaa cgcaggggag attcgctttg ataatcagac gacaccgaat gccgcgctga
     1681 gccgtgctgt tgcaaaaagt aactccccgg taacgttcca taaactgacc accacgaacc
     1741 tcaccggcca gggcggcacc atcaatatgc gtgttcgcct tgatggcagc aatgcctctg
     1801 accagctggt gattaatggt ggtcaggcaa ccggcaaaac ctggcttgcg tttacaaatg
     1861 tcggaaacag caacctcggg gtggcaacca ccggacaggg tatccgggtt gtggatgcac
     1921 agaatggcgc caccacagaa gaaggtgcgt ttgccctgag tcgcccgctt caggccggcg
     1981 cctttaacta caccctgaac cgtgacagcg atgaagactg gtacctgcgc agtgaaaatg
     2041 cttatcgtgc tgaagtcccc ctgtatacat ccatgttgac acaggcaatg gactatgacc
     2101 ggattctggc aggctcccgc agccatcaga ccggtgtaaa cggtgaaaat aacagcgtcc
     2161 gtctcagcat tcagggcggt catctcggtc acgataacaa cggcggtatt gcccgtggag
     2221 ccacgccgga aagcagcggc agctatggct tcgtccgtct ggagggtgac ctgctcagaa
     2281 cagaggttgc cggtatgtct ctgacgacag gggtgtatgg tgctgcaggc cattcttccg
     2341 ttgatgttaa ggatgatgac ggttcccgcg ccggcacggt ccgggatgat gccggcagtc
     2401 tgggcggata cctgaatctg gtacacacat cctccggcct gtgggctgac attgtggccc
     2461 agggaacccg tcacagcatg aaagcgtcat cggacaataa cgacttccgc gcccggggct
     2521 ggggctggct gggctcactg gaaaccggtc tgcccttcag tatcactgac aatctgatgc
     2581 tggagccaca actgcagtac acctggcagg gactctccct ggatgacggc caggataacg
     2641 ccggttatgt gaagttcggg catggcagtg cacaacatgt gcgtgccggt ttccgtctgg
     2701 gcagccacaa cgatatgacc tttggtgaag gcacctcatc ccgtgacacc ctgcgcgaca
     2761 gtgcaaaaca cagtgtgagt gaactgccgg tgaactggtg ggtacagcct tctgttatcc
     2821 gcaccttcag ctcccggggt gacatgagca tggggacagc cgcagccggc agtaacatga
     2881 cgttctcacc gtcccggaat ggcacgtcac tggacctgca ggccggactg gaagcccgta
     2941 tccgggaaaa tatcaccctg ggcgttcagg ccggttatgc ccacagcgtc agcggcagca
     3001 gcgctgaagg ctataacggt caggctacgc tgaatatgac tttctgatta tacggtatcc
     3061 cggaaatacg atttcaggaa aattcatgca gtaatgggaa aggacgctca ttgctgttca
     3121 ccgcagttct gaattattga atttataaag gtgaatgatg cacgaaaaga acatcgccct
     3181 gctttgtgat gaagccgacc ggcttttgca actgaatatc aatctgctcc ggcaaatggt
     3241 tgatgagccg gatgtgttat ctgacagtaa gaacgaaaac gggctgcttt ttgataaacg
     3301 gaaagcgctg aaaagaattg aggagctgga gggcgaacaa atcaaaaccg cccgcaggga
     3361 gatggtgctg gctgtggtcg gcacgatgaa ggcgggcaaa tcaaccacca tcaacgccat
     3421 tgtggggcag gaaattctgc ccaaccgtaa ccgccccatg acctcggtac cgacattgat
     3481 ccgccacgtt cccggaaaaa cggaaccggt cctccatctg gaacatattc agcccgtccg
     3541 caatttatta atcacactgc aggaaaaact cgccaccccg gcagggcagc aagtcgcaca
     3601 gtccctgcag caaaccgggg atacccgcga actactggat attctggcgg atgatgtctg
     3661 gcttaaaaat gaataccacg gggaagacga aatctttacc ggactggcat cgttaaatga
     3721 cctggttcgt cttgctgcgg caatggggac tgaatttccc tttgacgaat acgcagaagt
     3781 gcagaaacta ccggtgatcg acgtggcgtt cagccacctg gtgggaatgg atgcatgtca
     3841 gggaacactc acactgctgg atacccccgg ccctaatgaa gccggacaac cacagatgga
     3901 aatgatgatg cgggatcaac tacagaaagc ctctgcggtg ctggcggtga tggattacac
     3961 ccagatgaac tcgaaagccg atgaggaggt tcgtaaagag cttaatgcca ttgctgacgt
     4021 ctcagccggt cgtctgtttg tgctggtcaa taaattcgat gaaaaagatc gcaatggtga
     4081 tggtgcggat gccgtacgcc agaaggtgcc agcaatgctg aacagcgatg tgctgcccgc
     4141 ctcccgcgtt tatcccggct catcacgtca ggcgtacctg gctaaccgtg cgcttcatga
     4201 gttacgaaaa aacggtgcgc ttccggttga tgaagcctgg gtcgatgatt ttatcaggga
     4261 agctttcgga cctatggtgg aagaggacga ctggaaagac agcactaagg taaataaaaa
     4321 ggcagaaaaa ctctggaata tatctctgat cgatcaactg ataacggaag tcatacagag
     4381 ctcccattcc agagcggcgg cactggcggt tgactctgcc gccgcaaaac tgatgcagaa
     4441 tgcagaaaat gtcagcgaat atctgtcatt acgtcaatca ggggctgcag caaagcattc
     4501 agtcactaca ggcgcatatt accagcctgc tggcggatat ccaggagatt gaggaatgtc
     4561 agaatcaggt gaccggtgat gtcagaatgg ccatggagga catcaatacc aaaaccgggg
     4621 aattactgac gaaggtctgt gcctcactgg aggaggagct gaacgactat ttcagaagcg
     4681 gtaaacgcaa agaacagcaa atgctggagg aagaaaattc agcgcagccc cgggagagaa
     4741 acgcattcgc tttctttcac gatattttcg gaaccggaaa tcagcatgac aggatgcggg
     4801 actttgatcc ggacagcccc gaaataaaat tcagtgatcg cagggcagcc cttgaactga
     4861 tgacgcaaat cgaatcgacc gtgaccagcc tgcaccgtga ggctgaagca cagttccggc
     4921 ctgagctgga gaaaatcgtc agcgggattg aaacaggttt tcgtggcacg gccctgtacg
     4981 ccacagaaaa cattgccggt cgcatcaatg cccgcctgga ggatgagggc tttaccgtaa
     5041 aaatcagttt tccggcagtc agccagttac agacccggct cgcggtaaaa ataaatctga
     5101 gtgcgcttat ggaggaaaga acggagaccg tcacccgtcg ccgtcggcag agtggcgtat
     5161 ggggaaccgt ttgtcgatgg tttggcacca gtgacctggg ctgggaaaac tatgacgagg
     5221 atgtgagtcg cagcgtgatc aatatcaaca aggccagaga ggaagttatg tcactgaccc
     5281 gggcatattt cggggagctg caggcatcca ttgagcagga tattaaccag cccgtccgcc
     5341 aggaaatcga tgcctttttc tgcgcattca gggagaaagt tgaacaactg cgtaacacgc
     5401 tgattcagag ctctgaagat cataaacgcg atcagcaggc gcaggaacgg cttaccgggc
     5461 gacttcaggc attaaatgaa agggttcctg agctcattac tgacagtaag gcgctgaggg
     5521 aagagctgga gacaatgctg tgacttcacc atttattcaa caaattgctg ataaccgggt
     5581 atgtcaggta ctcacctgtc ttcctgaaaa atttgtggtt gatttcgcaa acggaatcga
     5641 tgttgcacag gagcatatcc gcacggccgg agagcgcacg ttcttccggc gtttaaaaga
     5701 ggggctgacc ggcgaaggcg ctgcacggca gaatgccatc aatgcgtcgc ttgcacaggg
     5761 cgttgaggcg tccctgcgct ggctgacgga gatgacaacg tcacttgcca ccacaaacta
     5821 cgcgattacc cgggtaaacg acagggtcag ttcactggtc agtgatacag ccaggctggc
     5881 gcattattcc gcagatacgc gggagcagtt actcaccctg gccgatcagg ttcaccataa
     5941 actgaatcat cttgaagaaa aactccaccg tgttgaccag gttcagcggg cgcagttaca
     6001 tcttgagcag atattctcat ggtggagcgc cgggcgatat gcgtcatttt cccctgccgg
     6061 acgttgttat gtggcacttg aagagcttcg ctggggcgcg tttggtgatg tgatacgtca
     6121 gggcgaaaca ggccaggtta accagctact ggatattctc agacataaag cattaacgca
     6181 gatggcacag gagagtggcg gtagtgcaac cgtgcgtctg aacactctgg actggcttgg
     6241 tggtcagggc cgggagcagg cagataacga gtggcatgac gcgattaact ggctgggaga
     6301 ctggtgcagt gaagagcagc atccggtgat ctggtcaacc acgcaggctg cagaacactt
     6361 accggttcgc atgcctcgcc tctgctctgc agaacgcctc tctgaaagta tggtcgatga
     6421 aatatttcag aagggggctg catgagtaca gaaatgaaaa cggggctggt gctgtccggt
     6481 ggcggggcgg tgggcgctta tcaggcggga gtggttaagg cactggcaga gtgtggtaca
     6541 cagatcagca tggtttcagg gaccagcatt ggcgcattca atggtgccat tatcgcggcc
     6601 tctcccgatc tgtcagaagc tgccgtacgc ctggaggcgc tctgggagca tctggggaat
     6661 aatcaggtgc tgtcggtaaa cagattggtt tacttttcat tgctgaaaaa attgttccag
     6721 gcaatgaacc tctgccagat ccccggacgt gcaggagcac tgcttacgac gcttcttcgc
     6781 catatatcga caatcaacgg gtttgacaat ctgatggctc agccgttgtt gtcagatgag
     6841 cccctgacag cgctgatgga tcattatctt gatactgatg ctctggcaga cgggctaccg
     6901 ctgtatgtgt cgctgtaccc cacagaaggg ggcatgcagg atattattga ctgcattcgt
     6961 gctgaactgg gtgtcggaac cacgaaaaac gccgtttttc agcatatcca gagcctgccc
     7021 cgcggacagc agaaagaggc tctgcttgcg tcagccgcgc tgcccctgct gttccgtccc
     7081 cgtgaggttc aggggacaat gttcggtgat ggtggtatgg gaggatggcg aaatatgcag
     7141 ggaaataccc ctgtgacgcc tctggtcgat gccggatgca atatggtgat tgtgacgcat
     7201 ctgagtgacg gttctttatg ggatcgccag gcttttccgg acaccacaat ccttgagatc
     7261 cgtccccgga aaaggctgaa atatgcaggt gatggtggca acagcggcgg tctgctcagt
     7321 tttacatcgg cacataccga cgcctggcgt cagcagggct atgaagacac gatgctggcg
     7381 atggagcata tccggaaacc gctggcagca cgtcaggcac tgacccggtc agaggcggta
     7441 ttgcagaaaa gcctggatat aacggaagag gcagatttgg cactgagaaa
//
LOCUS       DAZKGS010000199         7410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKGS010000199 DAZKGS010000000
VERSION     DAZKGS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            323..1945
                     /gene="yhiJ"
                     /locus_tag="ACHOQY_004307"
     CDS             323..1945
                     /gene="yhiJ"
                     /locus_tag="ACHOQY_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9680278.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2135..2248
                     /gene="yhiY"
                     /locus_tag="ACHOQY_004308"
     CDS             2135..2248
                     /gene="yhiY"
                     /locus_tag="ACHOQY_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9680279.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2311..3378
                     /gene="yhiI"
                     /locus_tag="ACHOQY_004309"
     CDS             2311..3378
                     /gene="yhiI"
                     /locus_tag="ACHOQY_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9680280.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3375..6110
                     /gene="rbbA"
                     /locus_tag="ACHOQY_004310"
     CDS             3375..6110
                     /gene="rbbA"
                     /locus_tag="ACHOQY_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9680281.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6110..7234
                     /gene="yhhJ"
                     /locus_tag="ACHOQY_004311"
     CDS             6110..7234
                     /gene="yhhJ"
                     /locus_tag="ACHOQY_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9680282.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1819 a         1761 c         1991 g         1839 t
ORIGIN      
        1 acttgataaa aataacaaaa taatttattc ctcagggctt agttgttttc aatctcatta
       61 aaactccatg aatacggtgg tatgcctttt taatcctgcc agtatttcat aggttttctt
      121 aatcgattgt agaggtatgt actgatgacg cctctacaat ctttagggac tttctctgct
      181 atgagataag aaatttcata ttatgcttta aggctgaatg atttgtgcaa aatataatta
      241 tttttccatc gaacatgagg tgtatgtcat aagattaaat gttaactatg cattcctcgc
      301 ggtgaattat atgaggtaaa agatgaaaat tggaactgtg gcaggcacta acgacagtac
      361 aacgacaatc gcaacaaatg atatggtgca agagcatgtt actaacttca ctaaagaact
      421 gtttgggtat attgcaaatg gtataggtga tgatatatca agtattgcca ggacaatgct
      481 cggtgaagtt gtggaaaaaa ttgatgattg gcaaatagaa cgtttccaac aatctattcg
      541 agatgacaaa atttcattta ccatacaaac gaatcattca gaaaaatata gtatgctatc
      601 gggcatgcgc gctcatattc tacgtaggga taataattat cagtttattg tgacaattaa
      661 tagtataaat tacggctgct atcttgataa taccgatatt aattggtgca gcattgttta
      721 tttacttaat aatatgacag taaatgatga tgctaatgat gttgcagtta ctgagtcgta
      781 caaacccatc tggaactgga aaatatcgca atacaatgta tttgatatta aattcgaaac
      841 aatgataaaa ccccagttcg ctgacagaac ctatttctcc aattgttcac ctgttgatcc
      901 cacaagtacc agaccaacct attttggcga taccgatggc tcagtcggtg cagttttgta
      961 cgcgcttttt gcaacgggac atttgggaat catggctgaa ggagaaaatt tccttagcca
     1021 gttgcttaat atagaagatg aagttctcaa cgtattgcta agggagaact ttaatgagca
     1081 acttgacact aatgttaata taataatcag tatcctgaac agaagagaca ttgttttaga
     1141 aagtcttcag ccttatttgg ttattaataa agacgcagtc acgccatgta cgtttctggg
     1201 cgaccagact ggcgatcgat ttagtaatat ctgtggcgat aaatttatca tcgatttatt
     1261 aaaacgcata atgagtatca atgaaaacgt tcatgtatta gctggaaatc atgaaacgaa
     1321 ttgcaatggt aactatatgc agaacttcac gcgcatgaaa ccacttgatg aggataccta
     1381 cgctgggata aaagattatc ctgtatgttt ctatggcccc aaatacaaaa taatggcaaa
     1441 tcatcatggg ataacttttg atgatcagcg aaaacgttac attataggac cgataacagt
     1501 ctctagtgat gaaatgacca atgctctcga tccggtcgaa ttagcggcaa ttatcaataa
     1561 aaagcaccat gctgtaatta atggcaaaaa gtttaaaaat agccgggcta tctcttgtcg
     1621 atcctttaat cgttattttt ctgtttcgac agattacaga cctaaacttg aagcactatt
     1681 agcatgctca caaatgttag gtataaatca agttgttgca cataacggta atggtggaag
     1741 agaatgtata ggtgaaacgg ggacagtttt aggtcttaac gcccgtgaca gtaaacatgc
     1801 tggaagaatg ttcagcatgc ataattgcca aattaaccct ggtgcagggc ctgagataac
     1861 cactccctgg aagtcttacc agcatgaaaa aaacagaaat ggacttatgc ctttgattag
     1921 aaggcgcaca atgttacagc tctgaaccat agacaacatg aatattaatg ttactatctg
     1981 gttgagactg ttgataaaac gtaaaaagga atgctctgtt cctgcaactg aacaggcgtg
     2041 aaacgcgatc caacaggatg gataccgttt tgtcagccag ttttatagat cacacttatg
     2101 agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac tacccgtttt
     2161 cactaagcct tccccgttgg cgctcaatgc tctgcgcgct ggtcgtattt gtcgtttcct
     2221 tcttatcccg gatggaagaa tccgctgaac gtcgttcggc ggcagttgta acacgcgtcg
     2281 tttgcttaca gggattatgg cgggccgatt atggataaga gtaagcgcca tctggcgtgg
     2341 tgggttgtcg gggcactggc ggtggctgcc gtcgtggcgt ggtggctgtt gcgcccggcg
     2401 ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg aagcgacgga agtggatatc
     2461 gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca gtttgttcgc
     2521 gaaggtgaag tgctggcgaa gatggatact cgcgtgttgc aggaacagcg actggaagcc
     2581 atcgcgcaaa tcaaagaggc acaaagcgcc gttgccgccg cgcaggcttt gctggagcaa
     2641 cgacaaagcg aaagtcgcgc cgcgcagtcg ctggttaatc aacgccaggc agaactggac
     2701 tccgtagcaa aacgtcatat gcgttcccgt tcactggccc aacgaggggc tatttctgcg
     2761 caacagctgg atgacgaccg cgccgccgct gagagcgccc gagctgcgct ggaatcggca
     2821 aaagctcagg tttctgcttc taaagccgct atagaggcgg cacgtaccaa tatcattcag
     2881 gcgcaaaccc gcgtcgaagc ggcacaagcc actgaacggc gcattgccgc agatatcgat
     2941 gacagcgaac tgaaagcccc gcgtgacgga cgcgtgcagt atcgggttgc cgagccaggg
     3001 gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga cgtctatatg
     3061 accttcttcc tgccaaccga acaggcgggc acgctgaaac tgggtggtga agcccggctg
     3121 atcctcgatg ccgcgccaga tctgcgtatt ccggcgacca tcagttttgt cgccagtgtc
     3181 gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac ggctgaaact gatgttccgc
     3241 gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt caaaaccggt
     3301 ttaccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga cgacctcgtg
     3361 gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc gcgcaactgg
     3421 cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa caatattact ctcgatattc
     3481 cggcccgctg tatggtcggg ctgattggtc cggatggcgt cgggaagtcg agcttattgt
     3541 cgttgatttc cggtgcccgc gtcattgagc agggcaatgt gatggtgctg ggcggcgata
     3601 tgcgcgaccc gaagcatcgc cgcgacgtct gcccgcgcat cgcctggatg ccgcaggggc
     3661 tgggcaaaaa tctctaccac accttgtcgg tgtatgaaaa cgtcgatttt ttcgctcgcc
     3721 tgttcggtca cgacaaagcg gagcgggaag tgcgaattaa tgagctgctg accagcaccg
     3781 ggttagcacc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg aagcaaaaac
     3841 tggggctgtg ctgtgcgtta atccacgacc cggaactgtt gattcttgat gagccaacaa
     3901 cgggggttga ctcgctctcc cgcgcccagt tctgggatct gatcgacagt attcgccagc
     3961 ggcagagcaa tatgagcgtg ctggttgcca ccgcctatat ggaagaggcc gaacgcttcg
     4021 actggttggt ggcgatgaat gccggagaag tgctggcaac cggcagcgcc gaagagttgc
     4081 ggcagcaaac gcaaagcgcc acgctggaag aagcatttat aaatctgtta ccgcaagcac
     4141 aacgccaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac gcagagattg
     4201 ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt gatcacgtca
     4261 atttccgcat tccacgcggg gagatttttg gattccttgg ttcgaacggc tgcggtaaat
     4321 ccaccaccat gaaaatgctt accggactgc tgcccgccag cgaaggtgag gcgtggctgt
     4381 tcgggcaacc ggttgatcca aaagatatcg atacccgccg tcgggtaggc tatatgtcgc
     4441 aggcgttttc gctctataac gaactcaccg tgcggcaaaa ccttgagtta catgcccgtt
     4501 tgtttcacat cccggaagcg gaaattcccg cgcgagtggc tgaaatgagc gagcgtttta
     4561 agctcaacga cgttgaagat gttctgccgg agtcattgcc gctcggcatt cgccagcggc
     4621 tttcgctggc ggtggcggtg attcatcgcc cggagatgtt aatcctcgat gaacctactt
     4681 ctggcgtcga tccggtggcg agggatatgt tctggcagtt gatggtcgat ctctcgcgcc
     4741 aggacaaagt gactatcttc atctccaccc actttatgaa cgaagcggaa cgttgcgacc
     4801 gcatctcact gatgcacgcc ggaaaagtgc ttgccagcgg tacaccgcag gaactggttg
     4861 agaaacgcgg agccgccagt ctggaagagg catttatcgc ctatttgcag gaagcggcag
     4921 ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga catcacccac gcgccgcgtc
     4981 agggatttag cctgcgccgt ctgtttagct acagccgccg cgaagcactg gaactgcgcc
     5041 gcgatccggt ccgttcgacg ctggcgctga tgggaacggt gatcctgatg ctgataatgg
     5101 gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgctcgac cgcgaccaga
     5161 ccgtcagtag ccaggcgtgg acgctcaatc tctccggttc ccgttacttt atcgaacagc
     5221 cgccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat atcacggtgg
     5281 caattgagat ccctcccaat ttcgggcgcg atatcgcgcg tggtacgcct gtggaactcg
     5341 gcgtctggat tgacggagcg atgcccagtc gtgccgaaac ggtaaaaggt tacgtgcagg
     5401 caatgcacca gagctggtta caggatgtgg cgagccgaca atccacaccc gccagccaaa
     5461 gcgggctgat gaacattgag acgcgttatc gctataaccc ggacgtaaaa agcctgccag
     5521 cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg ctaagcgccc
     5581 ttagcgtggt gcgggaaaaa gagcttgggt cgattatcaa cctttacgtg acccccacca
     5641 cgcgtagtga atttttgctt ggtaaacagt tgccatacat cgcgctgggg atgctgaact
     5701 ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt accgcataaa ggcagtttcc
     5761 tgacgctcac cctggcggcg ctgctgtata tcatcattgc caccggaatg gggctgctga
     5821 tctccacctt tatgaaaagc cagattgccg ccattttcgg aacggcgatt atcacgttga
     5881 tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa gggcctggac
     5941 gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccgc gggacgttct
     6001 cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg atagccatcc
     6061 cgctggtgat gggcttaagt atcctgctgc tgaaaaaaca ggagggatga tgcgccattt
     6121 acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg ataaagcgat
     6181 gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga ccgttacgcc
     6241 aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc agttatcgaa
     6301 ccggatcgtt aacagcttct atcgtccgtg gtttttgcca ccggagatga tcaccgccga
     6361 tgagatggat gccggactgg acgccggacg ctataccttc gcgataaata ttccgcctaa
     6421 ttttcagcgt gatgtcctcg ccggacgcca gccagatatt caggtgaacg tcgatgccac
     6481 gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca acggtgaagt
     6541 gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc tggaaacccg
     6601 gatgcgcttt aacccgaacc ttgatcccgc gtggtttggc ggggtgatgg caatcatcaa
     6661 caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg agcgtgaaca
     6721 cggcacggtg gaacacttgt tggtgatgcc gataacgccg tttgagatca tgatggcaaa
     6781 ggtctggtcg atggggctgg tggtgctggt ggtgtcggga ttatcactgg tgctgatggt
     6841 gaaaggcgtg ctgggtgtac cgattgaagg ctcgatcccg ctgtttatgc tgggcgtggc
     6901 gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag cgcgttcaat
     6961 gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc tttccggtgg
     7021 atccacgccg cgcgaaagta tgccgcagat ggtgcaggac attatgctga ccatgccgac
     7081 gacacacttt gttagcctcg cgcaggccat cctctaccgg ggtgccggat tcgaaatcgt
     7141 ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca ttgcactgct
     7201 gcgattcagg aagacgattg ggacaatggc gtaaatacaa ttgcccgatg cgtaagctta
     7261 tagggactac gcatcggtgc aatttattga attttagttg tttttaggcc ggataaggcg
     7321 tttatgaact gacccccaaa acctggacag tttcagttaa ccggcttttt gatactcagt
     7381 cctgtattca gggcaagatt acgaaagccc
//
LOCUS       DAZKGS010000200         7389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKGS010000200 DAZKGS010000000
VERSION     DAZKGS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..247
                     /locus_tag="ACHOQY_004312"
     CDS             <1..247
                     /locus_tag="ACHOQY_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680283.1"
                     /translation="ICTLCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKELSLT
                     LEFNDTIIESQKVTGNKAKHILEDIEIFYRNKPDTYY"
     gene            complement(469..1662)
                     /gene="tyrB"
                     /locus_tag="ACHOQY_004313"
     CDS             complement(469..1662)
                     /gene="tyrB"
                     /locus_tag="ACHOQY_004313"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9680284.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1897..2994)
                     /gene="alr"
                     /locus_tag="ACHOQY_004314"
     CDS             complement(1897..2994)
                     /gene="alr"
                     /locus_tag="ACHOQY_004314"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9680285.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(3047..4462)
                     /gene="dnaB"
                     /locus_tag="ACHOQY_004315"
     CDS             complement(3047..4462)
                     /gene="dnaB"
                     /locus_tag="ACHOQY_004315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9680286.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4545..5528
                     /locus_tag="ACHOQY_004316"
     CDS             4545..5528
                     /locus_tag="ACHOQY_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9680287.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5694..5936)
                     /gene="pspG"
                     /locus_tag="ACHOQY_004317"
     CDS             complement(5694..5936)
                     /gene="pspG"
                     /locus_tag="ACHOQY_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9680288.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(6070..7107)
                     /gene="dusA"
                     /locus_tag="ACHOQY_004318"
     CDS             complement(6070..7107)
                     /gene="dusA"
                     /locus_tag="ACHOQY_004318"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9680289.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1894 a         1929 c         1807 g         1759 t
ORIGIN      
        1 tatttgcacg ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac
       61 ctttttattt gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga
      121 gctctcttta actttagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa
      181 taaggcaaag catattcttg aggatataga aatattctat cgtaacaaac ccgatactta
      241 ttattaaatt taataatggt tagatgtgca gaaattatta ctccacccaa aaaaacagga
      301 taaggaattc cagataattg ggataagctt aacaataaaa gaactaacaa cgtttattat
      361 ctctcacgta gaacgatggc atcaaaaaaa tcatctataa agaaagagtg aaagcgacga
      421 cagtttaatt tcactgcagg ctgggtagct ccagcctgct ttcctgcatt acatcaccgc
      481 agcaaacgcc tttgccacac gttgtacatt tgccgtattt aacccggcga cacacatgcg
      541 accgctggcg atgagataga caccaaattc ttcacgtagt cggtcaacct gagcggcact
      601 taaaccggta taactgaaca tgccgcgctg attaagcaga taatcgaaat tgcgttctgg
      661 catctctgtg cttaatacct tcaccagttc ctgacgcatt gccagaatgc gagtacgcat
      721 ctcttctact tccgccagcc agctggcttt caatgcctcg tcattcagca ctgcagccac
      781 cacctgcgca ccaaaattcg gcgggctgga gtagttgcgg cgaactgttg ctttcaattg
      841 ccccagtacg cggccagcgg cttcggcatc ttcacacaga acagaaagtc cgccgacgcg
      901 ctcgccgtaa agggagaaaa ttttcgagaa cgaattgctc accagagcgg gtaatccagc
      961 gctggcaatg gcgcgaatag cgtaggcatc ctcttccata ccggcaccaa atccttgata
     1021 ggcaatatcg aggaatggaa taagctcgcg ggatttgaga atttcaatca ccgcatccca
     1081 ctgatcatta gtgagatcgg cacccgttgg gttgtggcaa catggatgca gcaacacaat
     1141 actgcgggca ggtaatgttt tcagcgtcgc caacaggtca ttaaagcgca cgccgttagt
     1201 cgcttcgtca taccaggggt aagtactcac ttcgaatcca gccccggcga atattgctac
     1261 gtggttttcc caggtaggat cgctgaccca gacgcctgat tccgggaagt agcgtttcag
     1321 gaaatccgcg cccactttca atgcccctga gccgccaagg gtttgaatgg ttgctacgcg
     1381 ctgttgttgc agtactggat ggtccgcacc aaacagcagc ggcgcaatgg catggcgata
     1441 gctgttaagc ccttccatcg gtaaataaag cgaagcgcca tgaggctgcg cattcaggcg
     1501 cgcttccgcc tccgccacgg cttgcagttg tggaataatt ccgtcttcgt tgtagtacag
     1561 accgatactt aaattcactt tgtcgctgcg agggtcttct ttaaaacgct ccataagcgt
     1621 aagaatcggg tcgccagcgt aggcgtcaac tttttgaaac acgcgatggt tctccaggtt
     1681 tacgggcagg tggttaaaac acaataaacc ggaagaaggc gaagatcgag tggatgttca
     1741 ggagcgaacg gcaattagca acagagtgag actcatgaca aacgtacatc cgccagaggc
     1801 acgaccttta taagagcatg aaagaacatc aacttattga atttttagga ttttattggc
     1861 cggataaggc attcacgccg catccggcac agacaatcaa atattacaga acgataaatc
     1921 cacgtatttc atcgcgaccc ttgaagtcag gcgcgtaata agttcgtaag cgcttacttt
     1981 cgtcatttca gcgatacgtt ctacgggcaa accttcgccc cataaaatga ccggatcccc
     2041 ggctttgtcc tgcgcttgtg gacctaagtc tacgcagatc atatccatcg ccacgcgccc
     2101 gacaatcggt acttcgcgac cgttcaccag cactggcgta ccggacggcg cggcgcgcgg
     2161 ataaccatcg ccatagccca tcgcgactac gccaagacgg gtatcacgtt cgcttaccca
     2221 ggttccacca taaccaacag gttctccggc tttatgctca cgcacggcaa tcaggctgga
     2281 ggttagtgac atcactggct gacagccaaa atcggcaccg gtggagcgat cttccagcgg
     2341 cgagacgcca taaagaatga tgcccgggcg cacccagtca aaatgcgact gtggccacag
     2401 cagaatgcca cccgatgcgg caatggaacg ttgaccaggt ttgccttcgc aaaaggtatt
     2461 aaagatagcg agttgtttct cggttgcgcc acattttggt tcatccgcgc gcgcaaaatg
     2521 gctgacgata ttcaccggct gacgaacgtt tttgcactgg gtcaggcgat gataaaacgc
     2581 ctcagcctgt tccggcctta cgcccagacg gtgcataccg gtatcgagtt tcatccagac
     2641 ggtaaccggc tcgtccaggc tagcctcttc cagcgcagcc agctgttctt cgttatgcac
     2701 ggcggtatga aaatgttgcg cagaaatcgt cggcagatct ctggcatcaa aaaagccttc
     2761 gagtaacagt acaggtttgg tgattccccc cgcacgcagt cgcagagctt cttcgagacg
     2821 ggctacgcca aaggcgtcag catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc
     2881 ataagcgttc gctttcacca ccgcaaccat tttactggca ggggccagtt cacgaagacg
     2941 ttgcaggttg tgtcgcagag cgcggcggtt aatcacaaca gttgccgctt gcatttgtgt
     3001 tccttgataa gtgtttgctt taattaccta attcataaaa taattattat tcgtcgtcgt
     3061 actgcggccc cgcatagttg tcgaagcgcg accattgacc gttaaaggtc aggcgtaccg
     3121 tcccgattgg gccgttacgt tgtttaccga taataatttc cgcgatgcct tttaaatcac
     3181 tgttttcgtg atacacctca tcacgataga taaacatgat caagtccgca tcctgctcga
     3241 tagagccaga ttcacgcagg tcggagttga ccgggcgttt gtcggcacgt tgttccagag
     3301 aacggttcaa ctgggacagc gccaccaccg gcacgttcag ttctttcgcc agtgctttca
     3361 gcgagcggga gatttctgca atttccagcg tacggttatc ggaaagcgcc ggtacgcgca
     3421 tcagttgcag gtagtcgatc atgataagcc cgatgccgcc gtgttcacgg gcaatacggc
     3481 gtgcgcggga acgcacttcc gttggcgtca ggccggagga gtcatcgata tagatattgc
     3541 gtttttcgag caaaataccc atggtgccgg aaatgcgcgc ccagtcttca tcatcgagct
     3601 gcccggtacg gattttagtc tggtcaacgc gcgacagcga cgccagagaa cgcatcataa
     3661 tctgttctga aggcatctcc agcgagaaga taagtactgg tttatcctgc aacatcgccg
     3721 cgttttcgac gaggttcatc gcaaatgttg ttttacccat cgacggacgc gcggcgacga
     3781 tgatcaaatc cgacggctgc aagccagcgg tttttttgtt gagatcgtca taaccggtgt
     3841 ttactccggt aacgccatcg tgtggctgct gaaacaactg ctcaatacgc gccacggttg
     3901 cgtcgagcac atcggcgatg ttcttcggcc cttcgtcttt gtttgcacga ctttcggcaa
     3961 ttttaaagac gcgggattca gcaaggtcca gcagatcttc gctggtacgc ccctgcggat
     4021 caaaaccggc ttcagcaatc tcattcgcaa ccgagatcat ctcacggaca acggcacgtt
     4081 cacgcacgat gtcagcataa gcactgatgt tcgccgcact tggcgtattt tttgacagct
     4141 ctgccagata agcaaaacca ccgacgctat cgagttgccc ctggcgttcc agcgattccg
     4201 caagggtaat cagatcgata ggactaccgc tttcctgcaa acgcgccatt tcagtaaaga
     4261 tatggcggtg tgggcgggtg taaaaatcgt ctgccaccac acgctcggct acatcgtccc
     4321 agcgttcgtt atccagcatt aaaccgccca acaccgactg ctccgcttcg atcgagtgcg
     4381 gaggcacttt cagcccggca acttgtggat cgcgttcgcg gggttcagcc tgctgtttgt
     4441 tgaagggttt atttcctgcc atagtgaatg gagttaccga gataaagaat gggtcgaaac
     4501 tttaccatat gaagcagccc ctgacgatac gttctggagg acacatggca acacgaattg
     4561 aatttcacaa gcacggtggc ccggaagtac ttcaagccgt agagttcact cctgccgatc
     4621 cggcagagaa tgaaatccag gtcgaaaata aagccatcgg catcaatttt atcgacacgt
     4681 atatccgcag cggcctttac ccgccgccat cactacccag cggattaggt accgaagcgg
     4741 caggcatcgt gagtaaagtc ggcagtggtg taaagcatat taaggcaggc gatcgtgttg
     4801 tctatgcgca gtcggcgtta ggcgcttaca gctctgtgca taacattaat gcggataaag
     4861 cggcgattct gcctgcggca atttcttttg agcaagctgc ggcatccttc ctgaaaggct
     4921 taacggttta ttatctgctg cgtaaaacct atgaaattaa acccgatgaa cagttcctgt
     4981 tccacgcagc ggctggcggc gttggcttga ttgcctgcca gtgggcaaaa gccctgggcg
     5041 cgaaacttat cggcaccgta ggaaccgcgc aaaaagcgca gagcgcgcta aaagcgggcg
     5101 cgtggcaggt aattaactat cgtgaagaga atctggtcga gcggttaaaa gagatcaccg
     5161 gcggtaagaa agtgcgcgtg gtgtacgatt ccgtgggcag agacacctgg gaacggtcgc
     5221 tggattgcct gcaacgccgc ggcttaatgg tcagttttgg caactcatca ggtgcggtta
     5281 ccggtgtgaa cttaggcatt ctcaatcaaa aaggctcgtt gtatgtgaca cgcccttccc
     5341 tgcaaggcta tatcaccacg cgggaggaat taaccgaggc cagtaatgaa ctgttctctt
     5401 tgattgccag cggtgtgatt aaggtcgatg tcgccgagca gcagaaatat ccgctgaagg
     5461 atgcgcagcg tgcgcatgag attctggaaa gccgggcgac gcaaggttcc agcctgctga
     5521 ttccataaaa gaaatagggc ttccacctgg gaagcccttt ctttttatag ttcggctgta
     5581 tgtagggtac agcacgatga atctgttaga ggcgcaatag tgacagattt gattatcaat
     5641 tcctattttg ttctaaggat aaaaccttag gttgtgatca tccgcacaat cccttagtaa
     5701 cgccagcggt cataacgctg atatttaggc acttttggtg ctttaatcgc cttaataacc
     5761 cacaccaccg caatcgccag tagtaaccac ggcagcaact taatcatcaa tgccagcata
     5821 ccgccgagga acataatggc cgtcgccaca accagcgcgg cgataatgcc cagcaacgaa
     5881 acgccggtga ccatcagcat gacaaaaaag ccaatcacaa aaagtagttc cagcatgatg
     5941 ctctcccaaa tatgaaatct cttgctggca ttacaagaat catgccaaaa ataatctatt
     6001 gatttaacag caaaacgccc cgcgacggtg cgcagggcgt ggtgaatttg actacttttt
     6061 ggtgaaaagt taacgcttat ccgccaccag tttgagcgcg tgttccagca cattaatgtc
     6121 tgcacccgct ttatgggcat tttcacttaa ataacgccgc cactgccgcg cgccaggaat
     6181 accctggaac aagcccaaca tatgccgggt aatatggccg agatacgtcc cctggctgag
     6241 ttcacgctca atgtacggat acatggcgcg cactaccgcc accggatcgg catcggtatc
     6301 cgaggaacca aagatctccc ggtctaccgc cgccagaata cccggattct gatacgcctc
     6361 gcgcccgacc atcacgccat ccatatgttg caggtgtgct ttggcctctt ccagcgactt
     6421 gataccaccg ttaatcgaca ttgtcagatg cggaaagtca cgcttcagtt gatacacacg
     6481 cggataatcg agcggcggga tttcacggtt ttctttcgga cttaaccccg aaagccaggc
     6541 tttacgtgcg tggatgataa acatctcaca ctcgcctttg ccggaaacgg tgttgatgaa
     6601 atcgcagaga aattcatagc tgtcctggtc atcgatgcca atacgcgttt tcaccgtcac
     6661 tggaatcgac accacatcgc gcatcgcttt cacgcagtcg gcaaccagct gcgtattacc
     6721 catcagacac gcaccaaaca tgccgttctg cacccggtca gacgggcagc cgacattcag
     6781 gttgatctca tcatatccac gcgcttctgc cagcttcgca cactgtgcca gcgccgccgg
     6841 atcgctaccg ccgagttgca acgctaccgg atgttcttct tcactgtacg ccaggtaatc
     6901 acctttaccg tgaataatcg cccctgtggt caccatttcg gtatacagca acgtattgcg
     6961 ggaaagcaga cgcaagaaat agcggcaatg tctgtccgtc cagtcgagca ttggtgcaac
     7021 gctaaaccga ccactccagt gaacgtcagt tttttcaggc attgcgctgg tttggttgat
     7081 tttttgcatt tcagaattac cgtgcatttt caaatgtaga gattatttta tcgatatatc
     7141 atggggttat gttattcagc atcactattc aggaggctca atagcggggt actataccat
     7201 aacaacagga agcgcctgtc tcattgcaaa agaaaattga gatcctctca aggcatgaag
     7261 ctctcacgaa gtgatggaaa taatcttatt agccgttaac tttgttaagg ccaatgataa
     7321 acaatccagg ttcgacgata aataaaaaat cacacattaa actctggtga tatatctccc
     7381 tgctaatag
//
LOCUS       DAZKGS010000201         7346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKGS010000201 DAZKGS010000000
VERSION     DAZKGS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..806
                     /locus_tag="ACHOQY_004319"
     CDS             57..806
                     /locus_tag="ACHOQY_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFP9680290.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            733..2855
                     /locus_tag="ACHOQY_004320"
                     /pseudo
     CDS             733..2855
                     /locus_tag="ACHOQY_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3025..4077)
                     /locus_tag="ACHOQY_004321"
     CDS             complement(3025..4077)
                     /locus_tag="ACHOQY_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9680291.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4392..5708
                     /gene="shiA"
                     /locus_tag="ACHOQY_004322"
     CDS             4392..5708
                     /gene="shiA"
                     /locus_tag="ACHOQY_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9680292.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            5810..7264
                     /gene="amn"
                     /locus_tag="ACHOQY_004323"
     CDS             5810..7264
                     /gene="amn"
                     /locus_tag="ACHOQY_004323"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9680293.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         1961 a         1719 c         1699 g         1967 t
ORIGIN      
        1 tgacaccaat ctttttcctt aacggaacga attgttgtgt agaaggagat aatattatgt
       61 caaaaaaata tcagctattg cttataactc attatatgtc aacatgggtc actataacgg
      121 aagcagttga gatcactacc aaagccataa aacaaaaaat tactcctagc gatatttatc
      181 gtcatgcctt gagtggcaac atcctactat tggtctattt ccagtctcct gtgatactta
      241 agaagataca aacttttaat ggaaaaataa aattcaggca gtttgtggga gacctacttg
      301 ataaactatg catgcttgac agggacggtt ttatttatgg tcaaaattta agactctgta
      361 cagaagccag atatatttgt cctgtgcaac aaattataga tacccccctg ttacgcaaac
      421 tcaaccagtt tcgtacgttt gttcgtaatg tccgcccggg tgatgaactg gatgtccagg
      481 cacaagttag tgaaaaaaat ttaaccccgc cgccgggtaa cagcagtggc aaccttgagc
      541 aacagatagc cagtacttca cagctaattg gatctctgct cgccgaagat atgaacagcg
      601 agcaagcggc aaacatagca cgtggatggg cctcttctca gacttcaggg gtaatgacag
      661 actggttaag ccgcttcggt accgcaagaa tcacgctggg cgtggatgaa gatttcagcc
      721 tgaagaactc ccgtgacggc aaccctcgaa aatggcgatt cgatgcaaca aacagtgaac
      781 tatgtgccga acgtcacgaa tgctgaaatc acgctggcag ccttgaagga tccggtgatt
      841 gccgacaata acgatctcac gacactaaca gcaccgtcgc tgatacagag ggcaatgcga
      901 tagccaacac tgaggtaaca tttactctac cggaagatgt gaaggcgaac ttcacgctga
      961 gcgatggcga caaagcggtt actgatgccg atggcaaagc gaaagtcacg ctgaaaggta
     1021 caaaagcagg cgctcatacc gtgacagcat cgatggttgg cggtaagagt gagcagttag
     1081 tggtgaactt tactgcggat acgctcactg cgcaggttaa tcttaacgtt accgaggaca
     1141 atttcatcgc caataacatt ggaatgacca ggctacaagc aatagtgact gatggaaacg
     1201 gcaacccagt agaaggtata aaagttaatt tccgtggaac ctccgtcacg ctaagcagca
     1261 ccagcgttga aacggatgat caggtttttg ctgaaattct tgtgacaagc accgaggtcg
     1321 gactaaaaac agtttcagcc tctctggcag ataaacctac tgaagtcatc tcgcgattac
     1381 tgaatgccaa ggtagatgtt aattctgcga cgattaccag tcaggagata cctgaaggtc
     1441 aggtcatggt cgcacaagac atagcagtta aagctcacgt taacgaccag tttggcaacc
     1501 cggtgacgca tcaagccgcg acattcagtg cagcgccatc ctcgcaaatg atcatcagcc
     1561 agaacacggt ctctactaat acgcagggag tagccgaggt caccatgacg tctgaaagaa
     1621 acggctcgta tactgtaaaa gcatccctag cgaatggagc ttcacttgag aaacaactgg
     1681 aggctattga tgaaaaactg acactcacgt ccagtccgct tatcggtgtc aatgctccta
     1741 aaggcgctac tctgacggca acgctaacct ctgcaaatgg cactccagtg gagggtcagg
     1801 tcatcaactt tagcgtaacg ctagaaggtg cgacgttaag tggcggaaaa gtgagaacca
     1861 actcttcagg tcaggctcca gtcgttctga ccagcaataa agtcggtaca tatacggtga
     1921 ctgcatcgtt ccataacggc gtcacaatac agacacagac aaccgtgaag gttactggca
     1981 acccaagcac cgcacatgtt gctagcttta tcgctgatcc gtcgactatc gccgccacca
     2041 acagtgattt aagtacctta aaggcaacgg ttgaggatgg cagtggtaac ctgatcgaag
     2101 gtctcactgt gtacttcgcc ttaaaaagcg gctccaccac attaacgtca ttaacagcgg
     2161 tgaccgatca aaacggaatc gcgacaacaa acgtgagatg agcgataaca gggagcgtca
     2221 ctgtaagcgc agtcataagc gcaggtggaa tgcaaacagt agatatatcg ctagtggcag
     2281 gcccagcaga cgcctcgcag tccatcctta agaacaacca gtcatcactg aaaggggact
     2341 ttaccgatag tgctgagcta catcttgttc tgcacgatat atcaggcaat ccgatcaaag
     2401 tttctgaagg gatggaattt gtgcaatcag gtacaaacgt gccctatatg aaaattagcg
     2461 caattgatta cagtcaaaat atcaatggtg attacaaagc tactgttaca ggagacggag
     2521 agggtatcgc aacgctgatc cccgtattga atggtgttca tcaggcgggc ctgagtacca
     2581 cgatagaatt cattagcgcg gaaaccagac caatgacggg tacagtgtca gtgaatagtg
     2641 caaacctacc gacagcttca ttcccttcgc agggatttac cggggcgtat tatcagttga
     2701 ataatgacaa ctttgcacca ggaaaaacgg cggctgatta ttcgttttca agctcggcct
     2761 cctgggtagg cgttgatgct actggtaaag tgacattcaa aaatgacggt gatagcaata
     2821 cagtaataat cacagcgccc ccaagatccg gtggtgctat ttaccaaaca gttcctcctg
     2881 aatcgcgttc ggtgtgacta tgatcatttt cggtgagtat tgctttgatc caaaggctgg
     2941 ttttctgtga tgattgacca gcctcagagt attgatgaca ccaccgttca taacagatcg
     3001 gcattactat gcataagtac tgcctcaaat tctcgccaaa acacttttta atgcctttgc
     3061 gaattcatcc agacttatat tgtcaatttt ttgcccaacg acgactgact ccgaaacatc
     3121 ggccattgcg ggccatcgcg ttttgtaatc agcataaatt gcaacaacag gggtattata
     3181 ggcacttgcc atatgtaata ttgaggtgtc aggagttatt gcaatataag catcttttat
     3241 gattgctgct gaacgtttaa tcgagggtga taaggataaa cggtagacat tattataaca
     3301 atccactaat gccctggctt tatcttcccc tttgggtcca taaacgatca ctattggaat
     3361 atctgtttca ctttgaattt ttgctattag attttctgca atcgataatg aaaatgtacg
     3421 ttcctgcgag ctaccgtcta aattgagcgc aatgtaagat cctaacgatc gcatttcctc
     3481 gcgtacctca tcgagtacat catcgcttaa tggcaattca tatattgacc tgactgccgg
     3541 aaaacccgcc tcacgcatca taaatgccca tgtcatgggt acaggagtcc ggagattctg
     3601 atccatccgg gacgcgttct tacacaaagg ggaatagcac ttcatggtta aaccgacgac
     3661 ctggaaattc gtttttgctt tcaactgact gataaatatc atcgttttca ggttcttttt
     3721 acgcatcgcc tctatacaaa gatccggcgt accgtattgc agtgttatat tcttcgctaa
     3781 atcttttatt tcttttaatg ttgcgtgatc ctgcatagtc attgtgacta atgttaattt
     3841 agtctgttca agtttgagcg cattaaagac ttctaaatta attgtcgacg taacaattaa
     3901 tagatgctta attttatgca attcaagcgc ccgaataaca ggaaagatgg ccatagcatc
     3961 gccaatctga tcgggaatat gtatgacaac aaagtctgtt ttttcaatat taaaattata
     4021 agctttataa tcgtagtaac taaatgcaat acgtctcaac aatgatacta aaaacataac
     4081 taacctcgcc tccctactgg ttataatgca atgcagtcta tcagaatcat cagggtgcaa
     4141 ttttgtgcat atgcggactt ttatgtttca tatctcgaac ctgtgggccc tctgcttaat
     4201 ccttaaacaa caccagcaac tcctgcgctt ttatcttcca tcgaattttt catgttgccg
     4261 ctaatcagcc ataaaatcat ttgcagatgc gctctgtcga ggtagtctca taaggttcgt
     4321 ttatagatcg acggcaatgt gagttacctt ttccatacta attataaaaa gacagtacaa
     4381 acaggatcat tatggactcc acgctcatct ccactcgtcc cgatgaaggg acgctttcgt
     4441 taagtcgcgc ccgacgagct gcgttaggca gtttcgctgg tgccgtcgtc gactggtatg
     4501 attttttact ctatggcatt accgccgcac tggtgtttaa tcgcgagttt ttcccgcaag
     4561 taagcccggc gatgggaacg ctcgccgcat ttgccacctt tggtgtcggc ttccttttcc
     4621 gaccgctcgg cggtgtcatt ttcggtcact ttggtgaccg gctgggacgt aagcgcatgt
     4681 taatgctgac cgtctggatg atgggcatcg cgacagcctt gattggtatt cttccttcat
     4741 tctcgaccat tgggtggtgg gcacctattt tgctggtgac actgcgtgcc attcagggat
     4801 ttgccgtcgg cggcgaatgg ggaggcgcag cgttgctttc cgttgaaagt gcgccgaaaa
     4861 ataaaaaagc cttttacagt agcggtgtac aagttggcta cggagtaggt ttactgcttt
     4921 caaccggact ggtttcattg atcagtatga tgacgactga cgtacagttt ttaagctggg
     4981 gctggcgcat tcctttcctg tttagcatcg tactggttct gggagcattg tgggtgcgca
     5041 atggcatgga ggagtccgcg gaatttgaac aacagcaaca taatcaagcg gccgcgaaaa
     5101 aacgcatccc ggttatcgaa gctctgttac gacatcccgg tgctttcctg aagattattg
     5161 cgctacgact gtgcgagttg ctgacgatgt acatcgttac cgcctttgca cttaattatt
     5221 caacccagaa tatggggtta ccgcgcgaac ttttccttaa tattggtttg ctggtaggtg
     5281 gattaagctg cctgacaatt ccctgttttg ccaggcttgc cgatcgtttt ggtcgccgca
     5341 gggtttatat cacaggtgcg ttaatcggaa tgttgagcgc atttcctttc tttatggcgc
     5401 ttgaagcaca atctattttc tggatagttt tcttctccat aatgctggca aacattgcgc
     5461 atgacatggt ggtgtgtgtg caacaaccga tgtttaccga aatgtttggt gccagttatc
     5521 gctatagtgg cgctggagtc ggttatcagg ttgccagtgt ggttggtggt ggatttacac
     5581 cttttattgc cgctgcactc atcacttact ttgccgggaa ctggcatagc gtcgccattt
     5641 atttgctggc tggatgcctg atttccgcaa taaccgcttt gttgatgaaa gacaatcaac
     5701 gcgcttgata gcctggcgaa gatcatccga tcttcgcctt acacttttgt ttcacattcc
     5761 tgtgacatac tatcggatgt gcggtaattg tatggaacag gagacacaca tgaataataa
     5821 gggctccggt ctgaccccag ctcaggcact ggataaactc gacgcgctgt atgagcaatc
     5881 tgtagttgct ttacgcaacg ccattggcaa ctatattaca agtggcgaat tacctgatga
     5941 aaacgcccgc aaacaaggtc tttttgtcta tccatcactg accgtaacct gggacggtag
     6001 cacaaccaat ccccctaaaa cgcgcgcatt tggtcgtttt actcacgcag gcagctacac
     6061 caccacgatt actcgcccta ctctctttcg ttcgtatctc aatgaacaac ttacgttgct
     6121 gtatcaggat tatggtgcgc atatctcggt gcaaccctcg cagcatgaaa tcccttatcc
     6181 ttatgtcatt gatggctctg aattgacact tgatcgctca atgagcgctg ggttaactcg
     6241 ctatttcccg acaacagaac tggcgcaaat tggcgatgaa actgcagacg gcatttatca
     6301 tccaactgaa ttctccccgc tatcgcattt tgatgcgcgc cgcgtcgatt tttccctcgc
     6361 gcggttgcgc cattataccg gtacgccagt tgaacatttt cagccgttcg tcttgtttac
     6421 caactacaca cgttatgtgg atgaattcgt tcgttgggga tgcagccaga tcctcgatcc
     6481 tgatagtccc tacattgccc tttcttgtgc tggcgggaac tggatcaccg ccgaaaccga
     6541 agcgccagaa gaagccattt ccgaccttgc atggaaaaaa caccagatgc ccgcgtggca
     6601 tttaattacc gccgatggtc agggtattac tctggtgaat attggcgtgg gaccgtcaaa
     6661 tgctaaaacc atctgcgatc atctggcagt actacgcccg gatgtctggt tgatgattgg
     6721 tcactgtggt ggcttacgtg aaagccaggc cattggcgat tatgtacttg cacatgctta
     6781 tttacgcgat gatcacgttc ttgatgcggt tctgccgccc gatattccta ttccaagcat
     6841 tgctgaagtg caacgtgcgc tttatgacgc taccaagtta gtcagcggca ggcccggtga
     6901 ggaagtcaaa cagcggctgc gtacaggtac tgtggtaacc acagatgaca ggaactggga
     6961 attacgttac tcagcttctg cacttcgttt taacttaagc cgggccgtgg caattgatat
     7021 ggaaagtgca accattgccg cgcaaggata tcgtttccgc gtgccatacg ggacactact
     7081 gtgtgtttca gataaaccgt tgcatggcga gattaaactt cccggccagg ctaaccgttt
     7141 ttatgaaggc gctatttccg aacacctgca aattggcatt cgggcaatcg atttgctgcg
     7201 cgcagaaggc gaccgactgc attcacgtaa attacgaacc tttaatgagc cgccgttccg
     7261 ataataagtt ctgaaccttc ttatcaaaaa tagatgtgaa ggattaagta agacatctgg
     7321 caaatagccc gcaataaacg cgggct
//
LOCUS       DAZKGS010000202         7294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKGS010000202 DAZKGS010000000
VERSION     DAZKGS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(207..404)
                     /locus_tag="ACHOQY_004324"
     CDS             complement(207..404)
                     /locus_tag="ACHOQY_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9680294.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(636..1178)
                     /locus_tag="ACHOQY_004325"
     CDS             complement(636..1178)
                     /locus_tag="ACHOQY_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9680295.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(1187..1552)
                     /locus_tag="ACHOQY_004326"
     CDS             complement(1187..1552)
                     /locus_tag="ACHOQY_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9680296.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALMHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            complement(1553..2608)
                     /locus_tag="ACHOQY_004327"
     CDS             complement(1553..2608)
                     /locus_tag="ACHOQY_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9680297.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(2610..2882)
                     /locus_tag="ACHOQY_004328"
     CDS             complement(2610..2882)
                     /locus_tag="ACHOQY_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680298.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2829..3008
                     /locus_tag="ACHOQY_004329"
     CDS             2829..3008
                     /locus_tag="ACHOQY_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680299.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(3050..3205)
                     /gene="hokD"
                     /locus_tag="ACHOQY_004330"
     CDS             complement(3050..3205)
                     /gene="hokD"
                     /locus_tag="ACHOQY_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9680300.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            3763..4860
                     /locus_tag="ACHOQY_004331"
     CDS             3763..4860
                     /locus_tag="ACHOQY_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFP9680301.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRD
                     MEIRVIPVSSPTRPSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINHLGIPLSNI
                     DIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVGL
                     YSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDF
                     WVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGN
                     YKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
     gene            complement(4820..5398)
                     /locus_tag="ACHOQY_004332"
     CDS             complement(4820..5398)
                     /locus_tag="ACHOQY_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFP9680302.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(5704..6015)
                     /locus_tag="ACHOQY_004333"
     CDS             complement(5704..6015)
                     /locus_tag="ACHOQY_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680303.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(6008..6262)
                     /locus_tag="ACHOQY_004334"
     CDS             complement(6008..6262)
                     /locus_tag="ACHOQY_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680304.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(6259..6681)
                     /locus_tag="ACHOQY_004335"
     CDS             complement(6259..6681)
                     /locus_tag="ACHOQY_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9680305.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            complement(6722..>7294)
                     /locus_tag="ACHOQY_004336"
     CDS             complement(6722..>7294)
                     /locus_tag="ACHOQY_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680306.1"
                     /translation="VDPSNFDGSTVDKKLPIRGPMIDPDPSVLKPDPSDKRSSCPDAS
                     QPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRIVSLYEQAASYDGEI
                     TRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVKNIMSPAKLREKWDE
                     LVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
BASE COUNT         1877 a         1813 c         1537 g         2067 t
ORIGIN      
        1 tccaggcaat cagcgttgat taactcacaa ctggatattt ttacagtatt aatcatagat
       61 caataagcac ttctctgata ggctcatacc gcttttgcgc aaagcagatg ggcctgaggt
      121 ttgcttgtga ccccaacgca tgagcagatg gctggcaggt gccgctaaca cccaccagcc
      181 gcccattacc acaaattaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga
      241 taatctgcca gacccgccat aacaagctga gtcagtatta actggcagcg ttcgcgtgaa
      301 aggtaagtat tctgcgcaat ttccccgacg gtcgccggtt cggtgacgct taattcatta
      361 aacaccactc tggcggtttc ggtcatatcc tgctgtttta gcatgctttt ttcccttttc
      421 cggttaacgt gacataccaa tacctcttgt cgaaaaagcc agcaagctga aagaccagta
      481 ttcacaacta ccagcgcgtt taatgttctg tgccgttttt cagacataaa aaaacccgca
      541 taaagcgggt tctttcaggt gtccatgtct gctattcgcc tcgcggtata gctttgcgaa
      601 gcgtagctgg attgaaacag tttatgcgta aaaaatcaag acattttttg agcaaacgat
      661 tctcgcatag ggatgtatag cgcatattca gcaacagcca accaattagc aattcgcttt
      721 tcgcatgtac taaaacacca ttccgggtgc acctcattca acaattcagc cattttgcgt
      781 ttactcatcc cccgcccttc gtacctttgc cgcaggatat caatcaatcc aggataacgt
      841 gcaagcgctt tacttatcac cccatcaatg cgtaacgcct ctgcatcagt acagtgagac
      901 aaccagctct tctgtctgcc agcgatcatc tctcgcaaga atgcttccag ctctggttta
      961 tcaatccctg actccctgat tctacgcagg gcttcattga ttgcggtttt tgtcagtttt
     1021 ttggatgcca gcaactgatt gaacatattt cctggtttgc caccacctat atacgaccaa
     1081 cgcccccaca tccgtaattt cccctggatc cagacggctt ccagcgtttt tagacgtaaa
     1141 tgctcgccgc ttttgcctgt aatttccggg tatatcatat ttacgatcac tcactctcaa
     1201 ttttgtaaat cttcacgccc agccgtcccc caggaacgcg ctgaccgcgc acaatattga
     1261 tttcatcaaa ctgctcgtcg tctatgagaa gtccggcatg catcagcgca tccagtggtg
     1321 ctttcaggat attgtccagg tcacgacgac gtttatccgg tggctctgca ataatcttta
     1381 tcgccagcct tccggacagg tttaatttca gccgctgctg gcgaacaata agcgccacat
     1441 cacggcgata acgctcaccg gcttttgata caaaatatgt gctgccacga cgtcgccagt
     1501 aagtgttcac cgttggcggg taaggcaaaa caaattctat gcgttcagtc attcatgctt
     1561 tccacttcag gacacccgaa tttctcgcgt gcattaaaaa acgaatcagc aacaacagct
     1621 ggctgccgtg tttttcttca aaatctttta ccccggcgtg tagttcgcta tggcatttac
     1681 ggcacagcgg aataacaaac aaatcatcag cctttgttcc catccctccc agtccatgac
     1741 caatgatgtg atgcggatca tctgcctgat taccacacgt catgcatttc tgcgttttta
     1801 cccaacgcgt gtatacaggc atctcttccc gtcgtggttt ctggcgctgg agatactgag
     1861 ccggtgactc cggatcaacg gcaatgctta ccaccgtctt ttcctgtggc gggttttgct
     1921 ggtgggcgtg aggcagtagc gcaatatttt ttgtgcgctg cttcagcatg ctggtggcgg
     1981 tctgctctcc cggcacgatg tcgctctcgc ggtatactga gcgaattttt tccgcgcgta
     2041 atcccagtga acgacgtaac accgtctccg gtagtgcgtc cgctacgtta tttatggttg
     2101 cccaccagga taattcagcc agcgataatt cccgctcctg cgtgccattc attgcgtggc
     2161 ggatgacatc aatcatccat gctgacaggt tttggtgagc aagctgcccg agtgattcgg
     2221 aagtctggtt acgcagctgg ttgtcgcagt gccagcacaa caccatcgcg ccggtaccgt
     2281 aacgatgtat gacggtttca ctgtgatggt agtcaccatg aggccactgg caggatttaa
     2341 tgtggcgtaa cagccagtca gacaatgcac cagcaccacc agcagcacga atcacccgct
     2401 catcgctgaa aaatggcagt aatgatttat cctccgccag cggctggcga acagcaggaa
     2461 cgactccgga cggcagaccg cgcatgcttt tcggttccgg ctccaccaga actcgagggt
     2521 tatgaaatac ctgcatggat tcacggcccg gttttagcac caccagccca agttccggta
     2581 ccggaacagg tcgaagtaat acccgcacgt tacctccaga tgcgttgctg gaatgtgcgg
     2641 gacggacgcg gtgggcgttc ggaataaggg agcctgacat agattatcca gtgacgataa
     2701 tcgaggctga gggctttctt aatctcgtat ccgcgtctgc ggtagttatg aattagccat
     2761 tcggcctgtt cttcagtaca tggtgggtgt tggtaccagt cggttttaaa tgcgtgtgaa
     2821 cgccgcccat gccggatggc aaggtcggta tcagaattgt gaaatttggt tttgtgcacc
     2881 atctgttttc tctgctggcg cagcaggtgt caggtgttca ggctgacgtg cgaattgtaa
     2941 accagaatgc caggaaaaaa caaaacccgc cgaagcgggt taagtgcggg tgcgttgagg
     3001 atgcctgact catcagaggt ggcgagggat ttctccctcg cctggtctct tactcctcag
     3061 gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt acctcgcaga
     3121 ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg atcagggcga
     3181 ttaacatcgc cttttgctgc ttcatagcct gcttctcctt gcctttcggc acgtaagagg
     3241 ctaacctaca tgtgcaaagc atgaaattgg cctcagatta atgttaagcg tcttgccgga
     3301 cgcgtaatgt taactggggc ttttctctat ctgccttttg gtgttcatgc ctgaggcaga
     3361 tagcctcaag cacccgcagc aattctaact atccaataga tcaatgccaa cttcttttcc
     3421 tgcaacatct ctcatcagaa gtgacccaaa ttcatccaat cagcacaaaa gaaacattta
     3481 aagcgtgaat gctaccgaac gttcatttca attacatcgt tttgattttt aacagttttc
     3541 acctcaacaa atcaaatggc attagcattc atcatggaag aaagactgat aaaaatgatc
     3601 attttcaatg acttatgatc acaccgggta ttgccaagtc attcctttta cgccaggata
     3661 cccacaagtg agtagtcgag gagagtttgc caatttttgc gatataaatt tcaatgtccg
     3721 aagaaccatt aattgttaat tgattctgta taaggtatta acatgacaat ttcatatatt
     3781 cctattctga aagcgaaacg ttctgagtta tcagctttat cgcagctatc cattgaaaag
     3841 aaatcaaaaa ttttgccatt actcgaaatt gaaccagtgc caattgaccc tgattcaggt
     3901 attgccttaa agagttataa cgagactctt atagagttcg gaaagaaagt ctcaaaatct
     3961 tgctcagata tgcaaggtgt ttatattgat ggattattaa ttgaagagca ttttatttct
     4021 cctgaagatc attaccctat aataaatgcg gttaatcaag ttagagatat ggagataaga
     4081 gttattcctg tcagttcacc aactcgccca tctaactata aaagagcgat tgatgaatta
     4141 atgcagaatg aaatatgctt gagattaacc acgttagatc tggttaaccc acaattaata
     4201 acgcattaca ttaatcatct gggaattcct ttatcaaata ttgatataat tattgactta
     4261 agagatgagt taaccgagga taaaattaat tccggcgaac tatatacttt ggcaatggga
     4321 ttgataaaca atctggcgca cctcaatgaa tacagaaaag tgattctttc tgggggttca
     4381 tttcctacag atctcagtga tatttctgtt ggtctatatt ctcaacctcg aatcgagtgg
     4441 attttatggc agagtttaat gaatagaaaa gaacttgcca gaaatgtgat ttatagtgat
     4501 tatggagtac agcaccccga ctttaatagg ctttcgacgc gattccctag cgtatctgcc
     4561 agcgtcagat actctggaga taatgacttt tgggtatttc gaggaagagt agctaatcgc
     4621 tttggttatg aacaatatgg taaacatagt gaggatattc ttgctcatcg agaatattca
     4681 gggcctacat tctgtgcggg agatagagac atagaatatt atgcaaacga gtatcaagcc
     4741 tacaaagcca atccctctgg taactataaa tttggtagcc cagaggtatg gcgtaggatt
     4801 gggcaaaacc atcacataac taaggttgtt gagcaactcg ccactcttta cgggctttaa
     4861 gtttaactcg aacagcttca cgaagctcgc caacatccat actattggcg agttttgacc
     4921 ataagacgcg tttaggttta tttttcaaat tctgtagctc ccctacatca cttaataggg
     4981 aaagcaattc atctttccaa agaagcattg ttaaggaaag tttgtccact tgtggattca
     5041 acttgctagt tcgaattgtt tttaaatgga taccttgccg tgcaccttgc gtaacttgct
     5101 ttatccccca ccatgatgga actatgctta aagcatcata aagatggcaa tcagagacaa
     5161 ccaaagttac tttatccatc actgatgagt aatgttgcac ctgcgctggt aatctgagca
     5221 agttatcact cttgctcttc aactcataac catgtataag cccattgatc actgctatat
     5281 cagctctgct agcccctaga ttcatcgtaa actcatcaat gattagggtg tcaggatctt
     5341 tatgatgatc tctcagaatc ttggcatgca cagcctttct tacatcaatg tctctcatac
     5401 catagttgcg ctttcatcga ctctctcaag ccatgattct atatcatggt tcgcatgaat
     5461 gccatttgat atgaccagtg gttaaacatt gcacagaaaa gtggatgtgt attttaacaa
     5521 aagcaatgac ggctaaccat gttggtatgt cgataaaatg tttcaacgta agcattatct
     5581 gttccgccct ttcagacggc ctcctgatgt tctgagggtg cagaatccct ccggttaagg
     5641 gtttaataaa aatcgtttct gatttaaatc ttcagtattt agttgttagt cggtttatag
     5701 cctttatgct tcggccttat ttctcagcca tacacaaacc gggccatctt cggtgtcatg
     5761 tattgaacca ataaaccatc cattgccctc tggtcgttcc ggttcccatg cagaaatatc
     5821 agcatcacac gcatcaaggt cagcacatcc ttcatctctg aagcagagga cgtattgaag
     5881 attattttcc tccatccagg cgttaaactc ttccgttgaa atatattccc gaccgtcaca
     5941 gaatttttca tattcaggat gcgtccagca gccatattca tcacgtacta ctggtatttc
     6001 tttaatttca ttcatttctg ttctcccacg ttttcagact ttcaccacag aacggacaaa
     6061 atgaaacccg aactggtaat ttagaaaatt caccggaacg caacatcaca aaatcaggac
     6121 cgcgagttaa actctcattc cagattttgt atatcagcag accttttcgc atcgtgtatt
     6181 cagcatcatg ctcaagggac tttgccagtg ctgcacatgg ttctatcttg ttgccattaa
     6241 cctggcattt tgattcactc accgcaccac ctcctcaaaa ttcccctgat aaaacgccag
     6301 tacgcgctgc ataacttcgc tcttccggca ctcgagacag attatgttca gacgcctgtc
     6361 gtagcggcgt atttcgccat ctggtaatga ccagataagg tccggatcaa ccgcagatgg
     6421 tttcttcggc tttgcccttg agagcttttt acgggcattt tgccagtcct tacgcgcctg
     6481 ttcagacggg aataacccgt aaccagagtt gtatacatcg ccactggcaa ccagctctct
     6541 ggccagaacg ctcatcagat atcttgttgc cccagtttta gtttccagtt gtcgtaacgt
     6601 ctcgcgccca ctctggcgta cgagttcaac aacctgccct ttaatttttt cccgctcttc
     6661 ttgtgtaaaa acttttgcca caagccctcc tgaaaattac ctcatgacca gaaattaaca
     6721 cttaccccct gaagcccggc ggaatttcag tgtccggttc agaaatgtga ttcacgcaac
     6781 gctgcgcagg cgaacgcccc aggcggataa ccagttcatc ccatttttcc cggagttttg
     6841 ccggactcat gatgtttttt acccagaacg aatcccgctg gagacgccca aacatttcac
     6901 aaatttgtct gtgagttctg ccatccagca tccgcattgt gcgaacgtca ttggcccatg
     6961 ctgtccagtt gggttctttc ggtctagtga tctcgccatc atagctggcc gcctgctcgt
     7021 aaagactcac gattcgtccc cagatccact gtgcgcacac caaatcttcc tgacttcccc
     7081 actggcgttt tttcgcactg aacacaaccg cgtcagggtg tcgggttaaa aaatcctgtt
     7141 cagccgtctg cgggtccggt tgcgaagcgt ccggacaaga agatctttta tctgacggat
     7201 caggttttaa tactgacgga tcggggtcaa tcatcggccc cctaatcggc agttttttat
     7261 caacagttga tccatcaaaa tttgacgggt caac
//
LOCUS       DAZKGS010000203         7271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKGS010000203 DAZKGS010000000
VERSION     DAZKGS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(83..844)
                     /gene="kduD"
                     /locus_tag="ACHOQY_004337"
     CDS             complement(83..844)
                     /gene="kduD"
                     /locus_tag="ACHOQY_004337"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9680307.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(874..1710)
                     /gene="kduI"
                     /locus_tag="ACHOQY_004338"
     CDS             complement(874..1710)
                     /gene="kduI"
                     /locus_tag="ACHOQY_004338"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9680308.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1997..3178)
                     /gene="yqeF"
                     /locus_tag="ACHOQY_004339"
     CDS             complement(1997..3178)
                     /gene="yqeF"
                     /locus_tag="ACHOQY_004339"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9680309.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3433..4662
                     /gene="yqeG"
                     /locus_tag="ACHOQY_004340"
     CDS             3433..4662
                     /gene="yqeG"
                     /locus_tag="ACHOQY_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9680310.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5122..5754
                     /gene="yqeH"
                     /locus_tag="ACHOQY_004341"
     CDS             5122..5754
                     /gene="yqeH"
                     /locus_tag="ACHOQY_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9680311.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
     gene            6088..6897
                     /gene="yqeI"
                     /locus_tag="ACHOQY_004342"
     CDS             6088..6897
                     /gene="yqeI"
                     /locus_tag="ACHOQY_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9680312.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGATTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            6890..>7271
                     /gene="yqeJ"
                     /locus_tag="ACHOQY_004343"
     CDS             6890..>7271
                     /gene="yqeJ"
                     /locus_tag="ACHOQY_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFP9680313.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFY"
BASE COUNT         2107 a         1665 c         1491 g         2008 t
ORIGIN      
        1 ataaccatga tatggattat gatgatctac aggtataaaa aaccctgcca tgcggcaggg
       61 tcataaaagt aagaagaatg aattaacgcg ccagccaacc gccatccacg gcaatggtat
      121 aaccattcac ataatctgaa gcgctggagg caaggaacac tatcggcccc atcaggtcac
      181 tcggcagtcc ccaacgacca gctggaatgc ggtcgagaat ttccgcgcta cgttgttcat
      241 ctgcccgtag ttgttgagta ttgttggtcg ccatgtaacc cggagctatc gcattaacat
      301 taatgttgtg tttagcccat tcgttcgcca tcaatcgcgt cacacccatc acgccgcttt
      361 ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt gacgcgatat
      421 tgataatctt gccgccattg ccttgcgcga taaagtgttt cgccgctgcc tgagacatga
      481 agaatacgct cttgatattc aggttcatga catcgtccca gtccttttcg ctgaactcga
      541 gagcatcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata tgaccaaact
      601 ccgctaccgc gcgatccagc agtccaggaa tgccatcaat ctttcgcaga tcggcggtca
      661 ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat ggtttcagtc ggttcaacga
      721 tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc ccctggccca
      781 gcccagtatc acaaccagtg acgaccgcaa ctttaccttc gagagaaaat gcacttaaaa
      841 tcataacaat accttattcg tttatgccca caactagcgc aaatctttaa cggccacatg
      901 gtccatatca tcaaagacct ggttttcacc gaccatgccc cagataaagg tataagcttt
      961 ggttccgacg ccggaatgaa tcgaccagct cggggagatc accgcctgct cgttatgcat
     1021 cacaatgtga cgcgtttctt gcggctgccc catcatgtgg aaaacgcagg cgtcatcatc
     1081 catattgaaa tagaaataaa cttccatccg gcgctcgtga gtgtgacacg gcatggtgtt
     1141 ccacaagtta cctggagcca gctccgtcag ccccatactc aattggcagg tttccagcac
     1201 atccgggaca aaatatttgt taattgtgcg acggttactg gtgaggttat cgcctaacgt
     1261 gactggagat acttcgtccg gtgtgacttt tttggtggga tacgtcgtat gcgcgggtgc
     1321 gcaattgtaa taaaactttg ccggagtggc ggtatcaata ctggcaaaga caacttcttt
     1381 tgcaccttta cccacataca gggcgtcgcg gtgaccgatt tcatagcatt ggccatcgac
     1441 agtaatcgta ccggcaccgc caatattgat aacacctaac tcgcgacgtt caaggaaata
     1501 gcttacgccg agttgtttac caacttcccc gccaacggaa accgttttag ttatcggcat
     1561 aatgccgcca acaataattc ggtcaatgtg gctgtaaacc atggtgtact catcggcgac
     1621 aaatacctct tcaaccaaaa attcattgcg cagcccttgg gtatccagcg tttttgcgtg
     1681 cgcactgtgg atgctctgtc ttacgtccac atcaacctcc gataatcagt gaagcgtgaa
     1741 aatagaaaac gatagtgaaa cactgtttcg ttttctctat aaagggatga tatgcccatg
     1801 ggctagggtt ttcaattaaa aataaaacaa tgtttcattt ttaagttaag aattaaaaaa
     1861 agtgcagtgt cgatcacgaa tatgtcatga gaatggaaaa atgagggagc ccagaaaatg
     1921 tatgtcgact tgccagccat cattattcaa cagattaatg acggcagata tttatcggta
     1981 aggaggaaat gcagagctac tcgtcacgtt caatggtcaa tgccacgccc tggcccccgc
     2041 cgatacaaag cgttgccagc cctttgcggg cattacgttt caccatttca tgaaccagag
     2101 aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc gagtgcgatc gcgccaccat
     2161 tgacattgac ccgacgctca tcccactcaa gcatcttgcc aaccgaaagc gcctgtgcag
     2221 caaacgcttc attagcctcg ataagatcga catcagccaa ctgccagcct acacgctcca
     2281 ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa tgccggatct acaccaacgc
     2341 tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgctcgtgct ttggcttcgc
     2401 tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc gcggtcacag
     2461 aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg gcgtcagtgc
     2521 gtggctgttc atcggtatca acaaccaacg tctgaccgtt actttggatc attaccggga
     2581 cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt tgcgagctaa
     2641 gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga ttttcggcgg
     2701 tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc agactgtcaa
     2761 ccaactggct attgccaagc tgtgcaccgg tgcggctatc agtcagaaca tgtggtgcgc
     2821 ggctcatgtt ttcctggcca ccggcgatga caatatcagc ctcgccacac tgtatcgcct
     2881 gagtagccag atgcagtgct ttaagcccag aaccgcaaac gtcattaata gtgattgcag
     2941 aaacgctatt aggaagacca cctttaatag ccgattgcct tgccggattc tgccctgccc
     3001 ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga acgccggtac
     3061 gttctattaa cgctttcacg accagactac caagttccac ggcggaatga cccgctaacg
     3121 caccgcgaaa gcagccgata ggtgtccgta acgccccgac aatcacaacg tctttcatca
     3181 caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt tatcttaatt
     3241 gtttaaaaaa agtgattttt atcacaaagg aaatatgcct gagcagtagt cagagtcata
     3301 actggcacgt aaggtttgca accactaacc caccaataga ggggtagata gggcgttaat
     3361 ctcccatact taacctggtt tatggtaaat tgccctccat tttgtttaat ttgtagatga
     3421 tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtggag tttcgcgctc
     3481 tacggcacag ccgttggtgc aggcacgctc ttccttccta ttcagttagg ttcggcaggg
     3541 gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatattg gccacataaa
     3601 gccttatgcc agttcatcct ctcatcgaaa acatcagcag gtgaagggat aacgggcgca
     3661 gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta cttcatcgcc
     3721 ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac ggaacagctg
     3781 gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta
     3841 atcctgaatc tcatttttct gatgggacgc catgcaacca ttcgggtaat gggttttttg
     3901 gtattcccat tgattgccta tttcttattt ctttccattt accttgtcgg tagttggcaa
     3961 cctgatctat taacgaccca agtagagttc aatcagaata cccttcacca gatatggata
     4021 tcgattcccg tgatggtttt cgcctttagc catacgccca ttatttctac gtttgccata
     4081 gacagacgtg aaaaatatgg cgaacacgct atggataaat gcaaaaaaat tatgaaagtc
     4141 gcttatctca tcatctgcat aagtgtactg ttctttgtct ttagctgcct gctttctatt
     4201 ccaccttcgt atattgaagc ggctaaagaa gaaggggtca ccattttatc ggcgctttct
     4261 atgctgccga acgccccagc atggctgtca atttccggga ttattgtcgc agtagttgcg
     4321 atgtcgaaat cattcctggg tacgtacttt ggcgttattg aaggtgccac ggaggtcgtc
     4381 aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg catttaaccg cgcactatca
     4441 attatgttgg tatcgctgat caccttcatt gtttgttgca ttaacccgaa cgcgatttcg
     4501 atgatttacg cgatcagcgg cccgctcatt gccatgatac ttttcatcat gcctacgttg
     4561 tcaacgtatc tgatcccggc gcttaaaccc tggcgttcca tcggaaatct gattacgctg
     4621 atcgtgggta tcctgtgcgt atcggtaatg ttctttagct gatatccatt gtgaatggtt
     4681 caaaattgct acaattttga accattccag ttttcataat gttcattcgt tattattccg
     4741 cactatcact tccctcatca tatcctagta ttatcagtta gttaaacaca caaagagatc
     4801 tgaacatctt aaataagact atttaagatg cataacttag attcgcaaga tatcttccgg
     4861 tcattcaaaa acaatttgcg atatagatat tctcatacca tgagatagtt atctgaacca
     4921 ccctcaacca ggtaagatta aatcagtgaa acataatcat atttaataac gagaaatgca
     4981 ttttaaaatg tcttaatcag aattatagca aatacagatt aatcgcaatt tttcccagca
     5041 aaattacaaa atcatggtgt attgacatca tcaatcacca atgaaattat gcaattatat
     5101 acggataggg aggttcttaa catggggctt tgtagtcgtt ataaaagtct tacatgcaat
     5161 agttgctcaa tgcattgcca aataatgcca gaagagtcac cgcgtttgca gtactgcgca
     5221 aactcgtgtt tttgtatgtg gcccgaagaa agttcatatt ttaatcgtgg tgtggtagaa
     5281 ggtatcttaa caaaaaacca caatgcgaga ttaagcggat atatttttgt cgatttttca
     5341 gtaagttttt tacgtctatt ccttgaaaaa gactggattg actatcttgc aagtactgac
     5401 atggggattg ttttagtcag cgaccgcaat atgcaatcgt tagctaatta ctggcgaaaa
     5461 cacaactcag caatatctgc tgtcatatat aatgatgatg gtcttgatgt tgccaatgaa
     5521 aagatcagac aactatttat tggtcgttat ttatcattta ccagaggtaa cacattaact
     5581 cagatggaat tcaccataat ggggtatatg gtttctggtt ataatccata tcagattgct
     5641 gaagttctgg atatggatat ccgtagcatc tacgcgtaca agcaacgaat cgaaaagaga
     5701 atgggtggta aaataaacga attatttatt cgttcacatt cggtccaata ttgatactca
     5761 acgaccagcc agaatcatac tctgtttata cgggcattta ttgaaatgtc cgtattttat
     5821 aaataggccg tcccctcaag agtaaacacc atagtattta aaaccatcac acgaaaaatt
     5881 cagaagcatt acgaaatatg aatttacaaa caatagtggc ataaatgtta accatgttaa
     5941 tttacgtaaa gttttacgtt gcaacattaa agcctcattt caatcatcat gataaatata
     6001 aaattaatat atatttatgc cgtaaatacc aatatactta gcaaactatg tgatctccat
     6061 ttcgattgat ttagtgttta ttgacgtatg tactggatta ttaacgataa tatcgagttc
     6121 tggcctgagc accgaaaatt aatatcggta cataacgccg atcttaacgt cgttctgaca
     6181 acgccagcca gtcgatgttt atcacttcta cttgaagctt ttcctgatgt ggttgcacaa
     6241 caagattttt tcaccagagt ctgggaagaa gaaggtatgc gtgtgcctac taacacgtta
     6301 tatcagaaca tatccattat cagacgcgga tttcgcgctg ttggtgctac tacccactcg
     6361 ctaattgcaa ccgtgccgag aagaggattc aagatccata atgacattaa catacaaaat
     6421 catgtaataa actcgtcaac agacgcacat acacacaatg ccccacctgc cataaaagtt
     6481 aatgcgggat acaaagagag cattggtggc gcaaagaatt tcaataacaa aatcctcaaa
     6541 catataaaaa gtcatctaat tatgttgagc gcatttgtca taggcgcata ttctgcatat
     6601 tggctatgga ataataatca accgaagcca tttttcaagg attacaagac tgttgcggaa
     6661 attaatggct gccattttaa tgtaacagaa gatacaattg acgggttgaa agagttcgat
     6721 aaatataaaa cacggatact ggattcgggg attaactgta aaaaacatcc gtggttatac
     6781 ttccctcttg caaaatcctc accggggatg attgttatgg catgcaataa aaattataac
     6841 caacatgaag tggcagactg tttaaccctg tcttaccgtg aggttaatcg tgattgatta
     6901 taaaaaaaac ttattattta ttttagtttt tatcagtggg tttattctct tcactgtcta
     6961 ttcttacact gcagaaaaaa tgatatataa cgaaacatgc actgcaaatt gggtaatttt
     7021 taatgaccag ggacgagcaa atcttaccat cgactttatg tataacaaaa aaaataaaac
     7081 aggaactgtt gcgttgagtg gaacctggca acaaggtaac agagaaagta aatcaatacg
     7141 gcgaaatatt gaatacacat ggattgaaaa ctatgacaca gcccatttaa catcaaagaa
     7201 agttaataag tttgagatta tggatcaagt tgatgatgat agacttgcac aacttattcc
     7261 tgatttttat g
//
LOCUS       DAZKGS010000204         7248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKGS010000204 DAZKGS010000000
VERSION     DAZKGS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..189
                     /locus_tag="ACHOQY_004344"
     ncRNA           58..189
                     /ncRNA_class="other"
                     /locus_tag="ACHOQY_004344"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(373..1050)
                     /gene="narI"
                     /locus_tag="ACHOQY_004345"
     CDS             complement(373..1050)
                     /gene="narI"
                     /locus_tag="ACHOQY_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9680314.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1050..1760)
                     /gene="narJ"
                     /locus_tag="ACHOQY_004346"
     CDS             complement(1050..1760)
                     /gene="narJ"
                     /locus_tag="ACHOQY_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9680315.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1757..3295)
                     /gene="narH"
                     /locus_tag="ACHOQY_004347"
     CDS             complement(1757..3295)
                     /gene="narH"
                     /locus_tag="ACHOQY_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9680316.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3292..7035)
                     /locus_tag="ACHOQY_004348"
     CDS             complement(3292..7035)
                     /locus_tag="ACHOQY_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9680317.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKISNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1622 a         2054 c         1878 g         1694 t
ORIGIN      
        1 cccaccacca tcacttttca aaagtccgta aactctcaaa cgaatccgca attaaatatt
       61 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
      121 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgttgagt aatccttccc
      181 ccaccaccat cacttcctta aataatcaaa acctgttact ccgagctaat tttgcccgat
      241 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
      301 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
      361 actaaaattc gcttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
      421 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
      481 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
      541 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
      601 atgctgagcg gagaacggaa tggtcaacag gcccagcgcg cactggataa cgagcagcga
      661 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acagacgacg
      721 cttcagcaac agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
      781 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcag
      841 cataccgaag aagtgaccaa caaaaatccc cagaataccg atatggaaca ggttcgacgc
      901 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
      961 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     1021 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     1081 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     1141 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     1201 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     1261 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     1321 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     1381 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     1441 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     1501 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     1561 tcgaacagtt cgctgtactg ggcctgggca tcgagcggat ccatcgccgt taaatcgcgc
     1621 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     1681 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     1741 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     1801 tacgacggct gttgaacaga ttgaatttgg tatctgaacc gtggcagcca tcaccaaagg
     1861 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     1921 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     1981 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacggttt
     2041 cagcacgttt gtaatgacgc atcgccaaca tacgtttcag tgcgcgcagt accggtttgg
     2101 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     2161 ctggcagaat gccgttgcta cccagctcac ccgcgtctgc tgcagactga atcggagaca
     2221 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     2281 gtttccattc cattgccatt ttataaaccg gcgactgctg tgcggcttca ataacgctca
     2341 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     2401 cctccagctg acgctggtaa agatctttct cgttctcggt gctggctgca cgttcaatcg
     2461 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     2521 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     2581 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     2641 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     2701 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     2761 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     2821 tgtctttcgc cagtttgtca aactcaccac ccaggtcatc ttcccagttc ggcccttttt
     2881 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     2941 tgctgccttc cggcgcggta tgcaggttct gatagtcaaa atcgaacggc tcgtaataat
     3001 cgtcgatccc cggcagatgc gggttagcga agattttacc cagcaacatg gcacggttac
     3061 ccatgcgcgg ctgcagtttg ccgttgattt tacggatcca gccgcctttg tatttttcct
     3121 ggttttccca gtcagtcggg aagccctgac ccggcttggt ttccacgttg ttgaaccacg
     3181 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
     3241 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
     3301 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
     3361 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
     3421 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
     3481 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
     3541 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
     3601 cagagcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
     3661 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
     3721 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
     3781 tttttccggg ttgccattgg atttctggcc catcacttct ttcaccgaac gggtgtcgat
     3841 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccattggt gatcctgata
     3901 cagctgctga cggccagaga gcgtacgcca cgggatcagc tcgtgaacgt tggtgtaacc
     3961 ggcgttgtaa gaaacgtgtt catcttcaag gccagaccag gtcgggctgg agatgatttt
     4021 gcgcggctgt gcctgaatat cgcggaagcg aatcttctcg tcttctttat tcagcgccag
     4081 atgcgtatgg tcacgaccgg taaactcgct cagggcagcc caggctttca cggctacctg
     4141 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
     4201 catcggctgg cctttcgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
     4261 catctcgctc tgggtgttcc aggcaatccc tttaccgcca ttactgattt tctccatcag
     4321 cgggccgata gaggtaaagc gttcgtaagt cgccggataa tcgcgctcta cgaccataat
     4381 gtgtggcgcg gttttaccag ggatcaggtc gcactcgcct tttttccagt ctttcacatc
     4441 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
     4501 ttctttaccc agatgaccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtaaat
     4561 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
     4621 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
     4681 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
     4741 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
     4801 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
     4861 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
     4921 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
     4981 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
     5041 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
     5101 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
     5161 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
     5221 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
     5281 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
     5341 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
     5401 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
     5461 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
     5521 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
     5581 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
     5641 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
     5701 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
     5761 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
     5821 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
     5881 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
     5941 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
     6001 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
     6061 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
     6121 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
     6181 gtagtctggt gtgacggcaa cagttttggt ccctttgtaa cgcacttcag taaagaagtg
     6241 agcatccggg gtacgcgtct gcggtacgtt tgacccccag gcgatgatgt agctggagtt
     6301 gtaccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
     6361 aggcaagtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
     6421 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
     6481 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
     6541 ctcctgccag gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
     6601 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
     6661 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
     6721 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
     6781 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
     6841 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
     6901 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
     6961 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
     7021 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
     7081 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
     7141 gtgtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
     7201 ttaaagcata acatgctgta aattaagacg ttttatacct gtagttat
//
LOCUS       DAZKGS010000205         7221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKGS010000205 DAZKGS010000000
VERSION     DAZKGS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHOQY_004349"
     CDS             complement(<1..114)
                     /locus_tag="ACHOQY_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680318.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHOQY_004350"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHOQY_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9680319.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHOQY_004351"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHOQY_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9680320.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHOQY_004352"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHOQY_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP9680321.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6741)
                     /gene="yehL"
                     /locus_tag="ACHOQY_004353"
                     /pseudo
     CDS             complement(5655..6741)
                     /gene="yehL"
                     /locus_tag="ACHOQY_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         1690 a         1953 c         1934 g         1644 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcacccat aactctgtca gaaagcatac ctaacagaca
     6241 atcttgcact tccagcggtg tacgcgttat ctcttcgaaa cggacgattt tgccgtcgcg
     6301 cattccctgg taaagcgggg cagggaccag cgcttccgtt gatgggccat gattgataag
     6361 cagagcgtag ttccagccgt acttgatttg atcttcagtg gtagatgcgc cgccctggat
     6421 ggttaacccg gcatcgccgc taatagcggt tgccagtaat tcagacagga gagattttgc
     6481 ggtgccaggt tcccccacca acatcaaacc gcgtccggtg gcaagagtga ctaacatacg
     6541 ttcgacagcg gagggactaa tcaccacttt acggctaata ttttgcgcgc tatcgccaag
     6601 gataaaagca cgggccgcag gcaaacttaa ctgccaaccg ggcgggcaag gtgcgttatc
     6661 attttgtttt aattttgcca gttcatcggc gtataacaca gcagcaggcg gacgctgcag
     6721 atgattattc tgcggtgaca tatattcatc cctgaaatgt attaatgaaa taaacgatat
     6781 tttacgcgca ataaatgttt ggcgcattag ttgtttgagc ggaaaatatc tggaaagcag
     6841 atcacagtaa ataaaaaaac ctgctggtga ggcaggttct tttatttact attttcaggc
     6901 ctaacaccac agcctgtgca acatcgtgtt ccaacgaacg aattataacc acggctggac
     6961 cccgttgcaa ttaagataat gagatttata atcctgactg ttgggacgtt gcgccggatc
     7021 gaacctattt caagtcctgg ctaccggacg gggggtgctg caggtggtgc cctgatccgg
     7081 agtgcaacat cgtggtccaa catacgtaaa ccagtatcga caggcagtgt aagggtaagg
     7141 tgctggtgtt gcgctcctta ataaggagag cggatggact cgctgacaca aaagttaacc
     7201 gtgctcattg ctgtactgga g
//
LOCUS       DAZKGS010000206         7218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKGS010000206 DAZKGS010000000
VERSION     DAZKGS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(156..1019)
                     /locus_tag="ACHOQY_004354"
     CDS             complement(156..1019)
                     /locus_tag="ACHOQY_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9680322.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(991..2541)
                     /locus_tag="ACHOQY_004355"
     CDS             complement(991..2541)
                     /locus_tag="ACHOQY_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP9680323.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2799..3578
                     /gene="deoC"
                     /locus_tag="ACHOQY_004356"
     CDS             2799..3578
                     /gene="deoC"
                     /locus_tag="ACHOQY_004356"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9680324.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3705..5027
                     /gene="deoA"
                     /locus_tag="ACHOQY_004357"
     CDS             3705..5027
                     /gene="deoA"
                     /locus_tag="ACHOQY_004357"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9680325.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            5079..6302
                     /gene="deoB"
                     /locus_tag="ACHOQY_004358"
     CDS             5079..6302
                     /gene="deoB"
                     /locus_tag="ACHOQY_004358"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9680326.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6382..7101
                     /gene="deoD"
                     /locus_tag="ACHOQY_004359"
     CDS             6382..7101
                     /gene="deoD"
                     /locus_tag="ACHOQY_004359"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9680327.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1684 a         1905 c         1968 g         1661 t
ORIGIN      
        1 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
       61 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
      121 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
      181 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
      241 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc gttcaggcgt
      301 accggaacat cgccaagtcc cttgataaac accgccagtt cttcgatgtg ttgcaaataa
      361 tccacctggc cagggattac cagcaaacgc agttccgcca gcttaccgcg ctctgccagc
      421 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgttg atgacattcg
      481 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
      541 ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg cagttgcgga
      601 tcgtttttga tggctgtaaa cagcgccacc acaaacggca gctgggtcgt ggcttcgccg
      661 ccgcttaccg ttatcccttc gataaacagc accgctttgc ggatatggct aagcacctca
      721 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacatctt caggcaggta
      781 tcacactgct cgcagaccac agcgttccac agcactttgc cgtcaacaat ctgcaacgcc
      841 tgatgcggac actgtggcac gcactctccg caatcattgc agcgtcccat cgtccacgga
      901 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
      961 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     1021 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     1081 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     1141 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa attcacgcat
     1201 tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac gtttgatggt
     1261 ttcgtcgagg gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     1321 agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg cgcccggcgt
     1381 ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     1441 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     1501 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct cacacagcaa
     1561 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     1621 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     1681 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     1741 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
     1801 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
     1861 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
     1921 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tctggatcgt agataaaggt
     1981 caggttcggt gaaacctgct tcaactctgc atctgcacgt aatatcgcac gggtgatggg
     2041 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
     2101 acgccagaaa cgttttattc gaatatcgat ctcgtcttgt gttagaattc taacatacgg
     2161 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
     2221 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
     2281 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
     2341 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
     2401 ctcggcaggc agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
     2461 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
     2521 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
     2581 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
     2641 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
     2701 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
     2761 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
     2821 gcctgcgtgc actgaaatta atggacctga ccaccctgaa tgacgacgac accgacgaga
     2881 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
     2941 tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa
     3001 tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg
     3061 cagaaacccg tgcggcaatc gcctacggtg cagatgaagt tgacgttgtg ttcccgtacc
     3121 gcgcgctgat ggcgggcaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
     3181 cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
     3241 aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa
     3301 cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
     3361 tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accggcgggc ggcgtgcgta
     3421 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgtttggt gctgactggg
     3481 ctgatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ctgaaagcgc
     3541 tgggtcacgg cgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
     3601 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
     3661 atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag
     3721 aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt cgtttcttta
     3781 tcaacggtat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
     3841 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
     3901 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact
     3961 cgaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg
     4021 gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt acgctcgaca
     4081 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
     4141 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
     4201 gtttctacgc gacccgtgat attaccgcaa ccgtggactc tatcccgctg atcactgcct
     4261 cgatcctggc gaagaaactg gcggaaggtc tggatgcgct ggtgatggac gtgaaagtgg
     4321 gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
     4381 gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac
     4441 tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg
     4501 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgctgatct
     4561 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg gtgctggaca
     4621 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact
     4681 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
     4741 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
     4801 tgggcggtgg acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata
     4861 tggcgcgtct gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
     4921 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
     4981 aagcaccgga aagcacacca actgtctatc gtcgtatcag tgaataacgg tatactgatc
     5041 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
     5101 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
     5161 ctgacaccct gggtcatatc gcagaagcct gtgccaaagg cgaagctgat aacggtcgta
     5221 aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
     5281 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
     5341 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atcgccggtg
     5401 tcccggttct gtttgagtgg ggatatttct ctgatcacga aaacagcttc ccgcaagagc
     5461 tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
     5521 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
     5581 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
     5641 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
     5701 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
     5761 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
     5821 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc cgacatctac gccaactgcg
     5881 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
     5941 agatgaaaga agcgggtgat aacactatcg tcttcaccaa cttcgttgac ttcgactctt
     6001 cttggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg ttcgaccgcc
     6061 gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc
     6121 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
     6181 tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatatcg
     6241 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
     6301 gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag agggtgaaaa
     6361 catgtagata aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc
     6421 gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcaa agtatattgc tgaaactttc
     6481 cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac
     6541 aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac
     6601 accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc
     6661 gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat
     6721 tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac
     6781 atggtgcgta acgcggtaga cgcggctaaa gcactgggcg ttgatgctcg cgtgggtaac
     6841 ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa
     6901 tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtggc tgcagagttt
     6961 ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca cgagcagacc
     7021 actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt
     7081 ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gaacgtcgtg ttaagccgga
     7141 gcaggagact gctccggctt tttagtatct attcatcaat agcaactgat ttatagattt
     7201 actattattt ttcatatg
//
LOCUS       DAZKGS010000207         7215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKGS010000207 DAZKGS010000000
VERSION     DAZKGS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(311..1377)
                     /locus_tag="ACHOQY_004360"
                     /pseudo
     CDS             complement(311..1377)
                     /locus_tag="ACHOQY_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1380..1742)
                     /locus_tag="ACHOQY_004361"
     CDS             complement(1380..1742)
                     /locus_tag="ACHOQY_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9680328.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2279..4192
                     /gene="mtlA"
                     /locus_tag="ACHOQY_004362"
     CDS             2279..4192
                     /gene="mtlA"
                     /locus_tag="ACHOQY_004362"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9680329.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4422..5570
                     /gene="mtlD"
                     /locus_tag="ACHOQY_004363"
     CDS             4422..5570
                     /gene="mtlD"
                     /locus_tag="ACHOQY_004363"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9680330.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5570..6157
                     /gene="mtlR"
                     /locus_tag="ACHOQY_004364"
     CDS             5570..6157
                     /gene="mtlR"
                     /locus_tag="ACHOQY_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9680331.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6169..6378)
                     /gene="yibT"
                     /locus_tag="ACHOQY_004365"
     CDS             complement(6169..6378)
                     /gene="yibT"
                     /locus_tag="ACHOQY_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9680332.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6663..7025
                     /locus_tag="ACHOQY_004366"
     CDS             6663..7025
                     /locus_tag="ACHOQY_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9680333.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1841 a         1807 c         1825 g         1742 t
ORIGIN      
        1 atatttttta aatgtgactg tcatccttca gataataaag ccatcatcgc aatagatgga
       61 aaagcactcc tgctcttttt tgattagagc cgtcgcagga ggaacaactc atatcatcat
      121 tgcgatctcg acgatgtata gtctcatcct gcggcagaac aagacggata aaaaatccaa
      181 tgagagcaca ctcatccaga acttattaac ataatgaata ttaaagaaaa atcatcgcag
      241 ctaatgaagt aatatgcaaa aataattagt ccttacaagg agcaaagttc cataatctcc
      301 cccctccccc tcaatgatcc aaataaagat aatgcatcca gctggtcatt cttagcagca
      361 ctttccgcat caccgaaaca tggctgaaat ggtcggagta aaccgccacc tgggcgtaga
      421 tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt cccagcacac
      481 cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc
      541 ctggcacgac aggtaaaata ctggtcagtt tgccgtggaa cacctgccca ggtagcgcgt
      601 taaacaccac ttctgcatca tcaccaggtt tcagacgtaa cagcgagttt tgccgaaatt
      661 gggcgacaat ttgccgtttt tgctcgggga tgaaaaccat caccggacgc aacggcaagg
      721 cagctgcgta tgtacctggg cggatcagta cctgagtaat gtagccattg ctcggcgcgc
      781 gaatgacagt ctgctcaaga ttatattttg cttcagtaag ttgcgctctt aagctcacaa
      841 tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg
      901 ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt
      961 cacgttccga gaacggattc accgccgcct ggctaccttt cagataacgt tgataatttt
     1021 taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tctgtgagct
     1081 gcgcgcgcag agtctttata ttatgcgtcg ccgtcatcag gtcagcctga agcctgtcaa
     1141 ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt tgaataagct
     1201 gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg atggggatcg
     1261 ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca
     1321 aaccactcac cagaaacacg ccagcgccac gtaagttaaa acaatcaata gatccataat
     1381 tagatctccg ggaacgtgga atagtcagtc tttttctcgg cggcgagttg atgctccagc
     1441 cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag tcgctttctc ctgcgacgca
     1501 acatgtgact gcatacccca gccacgctcc ggttgataga gcgtcgccca gatccacaga
     1561 aacggccaga taacatgcag agtaaacagg ctcacccagc ccgccgtatg aatagcgtcg
     1621 gcatggggat ggttgcgctt tttggcaatc agataaggga tgtcatgtat cgcgatgatt
     1681 ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata gtttagaaac
     1741 atatctgcct cggattcacg tttatcagtg ttgtttttgg gctggcagcc agaagggagt
     1801 caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac caaagaataa
     1861 tttttagagg tgatgagttg cttagttaca taacgattgt atgacgaagg cataacatgc
     1921 tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat cacaaagatt
     1981 caacaaacca tcaaaacaaa aatgtgacac tactcacatt taaatgccat ttttagcgaa
     2041 aatcgccgcc ttgttgcttt tttacacaag cgttttgtga tgaacgtcac gtcaattacc
     2101 tctctacccc ctatatttat gtgattgata tcacacaaaa ggccgtcgac tggacagtta
     2161 accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg ccgccacatt
     2221 aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg gtgtttttat
     2281 gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca acatggtgat
     2341 gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta ttccaacagg
     2401 gtggttaccg aacgagacgc tggcgaagct ggtcgggccg atgatcactt atctcctgcc
     2461 gctgctgatc ggttataccg gtggtaagct ggtaggcggc gaacgtggcg gcgtagtcgg
     2521 tgccatcacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc tcggttctat
     2581 gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg tagacggtaa
     2641 gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca tcgggatgat
     2701 cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt ccaaaatgct
     2761 ggctgcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt ctatctttgt
     2821 tgaaccggcg aaaatcctgt tcctcaacaa tgccattaac cacggtatct tctcgccgct
     2881 gggtattcag cagtcccatg aactgggtaa atcaatcttc ttcctgattg aagctaaccc
     2941 aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta gcgctaaaca
     3001 gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt atccacgaaa tctacttccc
     3061 gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc ctcggcggta tgactggcgt
     3121 gttcacgctg actatcctgg gcggtggtct ggtttctccg gcatctccgg gttctatcct
     3181 tgctgtactg gcgatgacac caaaaggtgc ttatttcgct aacatcgcgg gtgtgtgtgc
     3241 ggcgatggct gtctccttcg ttgtctctac tattttgctg aaaaccagca aagtgaaaga
     3301 agaagatgat attgaagcag caactcgtcg tatgcaggac atgaaagctg agtctaaagg
     3361 cacatctccg ctgtctgcgg gcgatgtgac taacgacctg agccacgtac gtaaaatcat
     3421 cgttgcctgt gacgccggta tgggttcaag tgcgatgggc gcaggcgtgc tgcgtaagaa
     3481 aattcaggat gcaggtctgt cacagatttc ggtcactaac agcgcgatca acaacctgcc
     3541 gccagatgtg gacctcgtca tcactcaccg tgacctgacc gaacgcgcta tgcgccaggt
     3601 tccgcaggcg cagcatattt cgctgaccaa cttcctcgac agcggcctgt acaccagcct
     3661 aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac gaagtgaaag tgaaagacag
     3721 tctgaaagac agctttgacg attccagtgc taacctgttc aaactgggcg cggagaacat
     3781 cttcctcggt cgcaaagcag caaccaaaga agaagcgatt cgttttgctg gcgagcagct
     3841 ggtgaaaggc ggttacgttg agccggaata cgttcaggcg atgctggatc gtgaaaaact
     3901 gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg aagcgaaaga
     3961 tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg gaaggcgtgc gcttcggtga
     4021 agaagaagat gacattgccc gtctggtgat tggtattgca gcccgtaaca acgagcacat
     4081 tcaggttatc accagcctga ccaatgcact ggatgatgag tccgtcatcg agcgtctggc
     4141 acacaccacc agcgtggatg aagtgctgga actgctggca ggtcgtaagt aatccaatcc
     4201 caccctctcc acatggagaa ggtggggtta attgcctgat gcgctacgct tatcaggcct
     4261 acaggatgca tcacaatttg ttgaatttgc acgttcttgt aggccggata aggcgcttac
     4321 gccgcatccg gcgctgcccc tctcctcacg gagagggttt gggtgaggga aaagcctcac
     4381 cccagccctc tcgggtaaaa acattgatga aggttaatac tatgaaagca ttacattttg
     4441 gcgcaggtaa tatcggtcgt ggctttatcg gtaaactgct ggcagacgcg ggtatccaac
     4501 tgacgtttgc cgatgtcaat caggtggtac ttgatgccct gaatgcccgt catagctatc
     4561 aggttcatgt ggttggtgaa accgagcagg tagataccgt ttccggcgtc aatgctgtca
     4621 gcagcattgg tgatgatgtc gttgatctga ttgctcaggt cgatttagtc actaccgccg
     4681 ttggcccggt tgtgctggaa cgtattgctc cggctatcgc caaagggctg gtgaaacgca
     4741 aagaacaagg taatgaatcc ccgctgaaca tcatcgcctg tgaaaacatg gtacgcggta
     4801 ccacgcagct gaaaggccat gtgatgaacg ccctgccgga agacgccaaa gcgtgggtag
     4861 aagaacacgt tggctttgtc gattccgccg ttgaccgcat cgtaccgcct tcggcttcgg
     4921 caactaacga tccgctggaa gtgacggtag aaactttcag cgaatggatt gtcgataaaa
     4981 cgcagttcaa aggcgcactg ccgaacatcc caggcatgga gttaaccgac aacctgatgg
     5041 catttgtcga acgtaaactc ttcaccctga acacgggtca tgctataacc gcgtacctcg
     5101 gaaaactggc cggtcatcag accattcgtg acgctattct cgacgagaaa atccgcgcgg
     5161 tggtaaaagg tgcgatggaa gaaagtggtg cggtactgat caagcgctac ggctttgacg
     5221 cagacaaaca tgcggcgtac atccagaaaa tcctcggtcg ttttgagaac ccgtatctga
     5281 aagatgatgt agagcgcgta ggccgtcagc cgctgcgtaa actgagtgct ggcgaccgtc
     5341 tgatcaagcc actgctcggt acgctggaat acagccttcc gcacaagaat ctgattcagg
     5401 ggattgctgg tgcaatgcac ttccgcagtg aagatgatcc gcaggctcag gaactggcag
     5461 cactgatcgc tgacaaaggt ccgcaggcgg cgctggcaca gatttccggt cttgatgcca
     5521 acagcgaggt tgtatccgag gcggtaaccg cttataaagc aatgcaataa tggtggacca
     5581 ggcgcaggac accctgcgcc cgaataacag attgtcagat atgcaggcaa caatggaaca
     5641 aacccaggcc tttgaaaacc gtgtgcttga gcgtctgaat gctggcaaaa ccgtgcgaag
     5701 ctttctgatc accgccgtcg agcttttgac cgaggcggta aatcttctgg tgcttcaggt
     5761 attccgcaaa gacgattacg cggtgaagta tgctgtagaa ccgttactcg acggcgacgg
     5821 tccgctgggc gatctttctg tgcgtttaaa actcatttac gggttgggcg tcattaaccg
     5881 ccaggaatac gaagatgcgg agctgctgat ggcattgcgt gaagaactaa atcacgacgg
     5941 caacgagtac gcctttaccg acgacgagat ccttggaccc tttggtgaac tgcactgcgt
     6001 ggcggcgtta ccaccgccgc cacagtttga accagcagac tccagtttgt atgcaatgca
     6061 aattcagcgc tatcaacagg ctgtgcgatc aacaatggtc ctttcactga ctgagctgat
     6121 ttccagaatc agcttaaaaa aagcctttca aaagtaagca acgtctgctt actgcccctc
     6181 tacctgcttc ggccgataaa gccgacgata atactccaga cgttgtagat ataacggcac
     6241 gctttcatcg ggtattccgg acggaatcgc gttacgggga ggaagttttt tcagatactc
     6301 ccggaacgcc tggcttgatg ccatgaaatc tacggcttta tcgataagaa gcggaacgtt
     6361 ttcacctaat ttgcccatga tgatatctcc gtattacccc gcgccgggaa tgcgcggccg
     6421 ccaattttag tttaggttcc cggtaaagat gacattaagg aaaacgtgct gaatcctcaa
     6481 taatcatccg ctgctgttta ttatcttttt tctatagatt tcttaattaa tcaacgaatt
     6541 gcgtagtttt tgagcatgtt aatgattacg ttgcagaact attacaatgt catcagttgt
     6601 gaacaaagca cctggtcgcg catactaggg gctataaatt tatctttatc agaagccatc
     6661 acatgaaaga agtcgaaaaa aacgaaatca aacgtctcag cgatcgcctg gacgccatcc
     6721 gccaccagca ggccgatctg tcgctggttg aagccgcaga caaatatgcc gagctggaaa
     6781 aagagaaagc cacgctggaa gcagaaattg ctcgcctgcg tgaggttcat agccaaaaac
     6841 tgagtaaaga agcacagaag ctgatgaaga tgccgtttca gcgcgcaatt accaaaaaag
     6901 agcaggctga tatgggcaag ctgaagaaaa gtgttcgcgg actggtggtt gtgcacccaa
     6961 tgaccgcgct gggccgtgaa atgggcctgc aggagatgac tggattctct aagactgcgt
     7021 tttaagcgcc tcagacgact ggttcgatca tatcaacccg catctggctt gccttagctg
     7081 gtcaatgcgg ggactctcct gtctactcgc ctaattacca aacaattcac tccctttacg
     7141 tcgatttacc gctggttgat attctgaaaa tgcattttta ccgaaatagc cacctataaa
     7201 caataaaaat atata
//
LOCUS       DAZKGS010000208         7176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKGS010000208 DAZKGS010000000
VERSION     DAZKGS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..1003
                     /locus_tag="ACHOQY_004367"
     CDS             68..1003
                     /locus_tag="ACHOQY_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9680334.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1059..1601)
                     /locus_tag="ACHOQY_004368"
     CDS             complement(1059..1601)
                     /locus_tag="ACHOQY_004368"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9680335.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1585..1896)
                     /locus_tag="ACHOQY_004369"
     CDS             complement(1585..1896)
                     /locus_tag="ACHOQY_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9680336.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2081..2971)
                     /gene="cyoE"
                     /locus_tag="ACHOQY_004370"
     CDS             complement(2081..2971)
                     /gene="cyoE"
                     /locus_tag="ACHOQY_004370"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9680337.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2983..3312)
                     /locus_tag="ACHOQY_004371"
     CDS             complement(2983..3312)
                     /locus_tag="ACHOQY_004371"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9680338.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3312..3926)
                     /locus_tag="ACHOQY_004372"
     CDS             complement(3312..3926)
                     /locus_tag="ACHOQY_004372"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9680339.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3916..5907)
                     /gene="cyoB"
                     /locus_tag="ACHOQY_004373"
     CDS             complement(3916..5907)
                     /gene="cyoB"
                     /locus_tag="ACHOQY_004373"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9680340.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5929..6876)
                     /gene="cyoA"
                     /locus_tag="ACHOQY_004374"
     CDS             complement(5929..6876)
                     /gene="cyoA"
                     /locus_tag="ACHOQY_004374"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9680341.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1968 a         1770 c         1782 g         1656 t
ORIGIN      
        1 tcattaaatg gatatttggt ggttaattat tatggaaata tatgacagtg aggacataaa
       61 ggtatacatg ttaactaaaa gaatattatt ttctgtaatg ctgatagttt caccaagcgt
      121 ggtggccagc gagaaagcgc atgaattgta tgacagcata tacggtggta aacctgctcc
      181 cgatgttata aatacactgc ataaaatggc tgaatcggga gatatcgacg cgcagagcct
      241 gttaggctgg gaatattatc agcctcgtta tgataccaaa cctgatgttc aggaagcgat
      301 taaatggttc gagttagtgg ctaaacaagg tgacagagaa gctccgttag cgctgggggg
      361 tatctactac gacggtgagc aggtgcgggt ggattatgcc aaagcgtatg cactatttaa
      421 tcaggccgcg cagcacggtg tgaatttagc aaggtccagg ttgggcatta tgtacgccaa
      481 tggtcagtat gttgaggtag attgtaagaa agcgaaggaa tatcttgata aaggtgtcca
      541 catttatggt ggcccagaag actttctggc tacttgtcga aaagacatga ttgacagaaa
      601 aacggttgac gatacgttac ccgtgattac ggttacccgt tctgggatgc gagataattt
      661 tttagataaa ggtttttcct gcatggatag cctcttcgcc accaccaata aattaggcga
      721 agtggccaat cttcgcgtga cattcagcat tcgtcgccca tccggtaagg agattaacca
      781 aacggttggc ttcgcgcctt tcggtttaaa ccggctgaat attagcttta ctgattacct
      841 atttggttca tttaccagta attcgtctct tattctttat aaaccggaat ttgagcgaaa
      901 atcttgtgca actgtaagga ccacaatcgt tgccgcaacg gcaaccatta acggcaagga
      961 tgtggagttg ctgaaagcgg gggcaattga acaaaagtgg taacgctgct cagcccccgg
     1021 ctgaggcaaa acttgccctg gcgggtggcg ataataatct aagccgcgag aatatgctga
     1081 atcgccaaaa acataggtac agcaccaaaa gcatttggtg tcgctgaaag ctgaacaaag
     1141 ccgagacgag cataaaaatt aatggcggca gggtcagcat caagataaac ccctttaatt
     1201 ggtaatgcct ggtgaattat ttttacatgt tcaaaaaaat cacatagtag gtcctgacca
     1261 aagccccgct tttgatactt ccgtgctacc cccaacatga ctaatctgac aacaccaatt
     1321 tctgaaggtt gtgaaccctg aaggacgcca gaaacgcgtt gtttttccag cgaatatgcc
     1381 gtaaaagtac agatgccgat cagttcacca ctttgtctgt caataagtgc tttagccgca
     1441 cagtcgctat tacgcacact tttctttagc gatgcgcgta caaatttatc gataacaggg
     1501 ttaccgcaat caaactgttt ttgcccagga taagtaatat cagactgata attacaggag
     1561 aggactatgg gcagagtaat ctcttcatgc ttgtcgacca tcgctgtttt tccttttcat
     1621 taacgccttg agggcgagcg tcggttgagc aggttcagcg atgagttggt tcatcagttc
     1681 ccagctttga ttcgaaagct cacggcgacg ttggttatca agcacacttt cggcgcgttc
     1741 catagcagcg ctcaaaataa aagcgcttaa atctacccca acggcttgcg cagcttcacg
     1801 aagtttgttt ttggcgtcgg gactagtttt gagttcgacc cgcgcatttt tcagcgtttt
     1861 atcgacatcg ggtagagtgg ctgtttttcc aggcattatg acctctttga ttaactgaat
     1921 gaaatcgtag ggagtcgatc tcatacataa tacggctatc aaccgtatga ttaatttatg
     1981 ttcatctgct cactctgtca agacatacgg ttattagccg tatctctgat ctcagacaat
     2041 aaaaaaggtg ccgtagcacc tttttaatag agaggttttg ttaccacaca gcagccagca
     2101 gcgtatgcga gtccggtacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg
     2161 cgatgataga gaagccgaac agcttgcgcg cccagattct gtcatcagca actttataac
     2221 cgcgcagagc catacctaac caccagacgc taaccgccgc ggcgaccacc agatatttat
     2281 atccagcgta accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca
     2341 gcgtgatgtg attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg
     2401 ccgcctggta atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca
     2461 ggctgaagat agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt actgcacagt
     2521 agccaatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagactgagt
     2581 ggcgtttcat gtacaggcta tagacgccga cataaaccac aaagcccatc acccccagcc
     2641 agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg
     2701 tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg
     2761 tcctttccat ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg
     2821 ccacaaccag tgacacccca accagcgtgt agataaacag gggataatca atgctgccct
     2881 ttgaggccag caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt
     2941 tcgttacttg caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt
     3001 gtagttgagg ttccacataa tccagatgga gcctacaacc aggatagcga tgattagcac
     3061 ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa
     3121 gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac
     3181 ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga
     3241 caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt
     3301 agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc
     3361 acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt agtgctggtc
     3421 aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc ggaagtgacg
     3481 tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct gcgatccgga
     3541 cccatgccgt taacaatcag gtgatggaat tcgtagattt ccatcccgat aaatccggca
     3601 ccaaacaacc aggtcaacgc cagccaggag ataacctggc ttttgttgtt tttgtacatg
     3661 gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga
     3721 acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc gttcaccaga
     3781 acggcatagg tagcaaacaa gattgagaac agaatgcagt cgctcatcag gtagatccaa
     3841 aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtgggcagtc
     3901 gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg
     3961 ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa
     4021 gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat
     4081 atgccagatc atggcgaaac cgaagatggt ggagaaagct gcaatgacga tacctgcacc
     4141 gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg cttcgccttt
     4201 ctctttcatt tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg caaagttata
     4261 gaacggaggc ggggaagagg ttgcccactc cagcgtacgg ccaccccacg ggtcgccagt
     4321 caggtcacgg ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa cgaggcagag
     4381 aatacccagc gcaatcagta ctgcaccgct agctgcaatc atcagcatgg tgtggaactg
     4441 cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg catacagcgg
     4501 cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac cccaggtttc
     4561 attcagtttg aaaccgaagg ctttaggcca ccagtaggtc atccctgcga agcagccgaa
     4621 gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc tgttatgcag
     4681 aacgaagtcc gcgcccggta ctgccagcag cacgccagtc atcccgccca ccgagaaagt
     4741 gacgataaaa ccgatggtcc acagcatcgc agaatggaac acgatgcggc cctgatacat
     4801 ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca ttgtggtgat
     4861 accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt gcagccaaac
     4921 gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat aaccaaacag
     4981 acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag gcaggatcag
     5041 gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca tcatgttgcc
     5101 acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca acaacgcgac
     5161 ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca gtgatgccca
     5221 ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca gaatggtaac
     5281 gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac tccatatcca
     5341 gtaatcgaca ccgactcccg gactgtactc tattcccgat agcggtggat aggccagcca
     5401 gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca caccaacaac
     5461 agtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg cgccgatctg
     5521 cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga agaagatcat
     5581 aatcacgccg tgcgcggtga agatctgatc gtagtggtga ggtggcagga agcccgcttc
     5641 gcccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa aaccacgcag
     5701 caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt cgacggaggt
     5761 cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc caacgagcgc
     5821 cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat ggaacgggac
     5881 tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt aatgggcgga
     5941 ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcactgtgct caccttctgg
     6001 ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaatcacat cggcaaacaa
     6061 gtctggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg cggccagttt
     6121 ttcgaacgca gccatgtcag acatggtgtt cggcgactgt ttcgcttttg cgacccattg
     6181 gtcgaattcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg agaagcccgg
     6241 cccgctgtag ctggcggaga taccgtcata agtaccgggt tcgttggcga tcagatgcag
     6301 gcgagtctgc ataccggcca tggcataaat ctggctaccc agacgcggaa tgaagaagga
     6361 gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg ggaaagcgat
     6421 ttcattcacg gtagcaatgc cctgttccgg gtagatgaag aaccatttcc agtccatgga
     6481 aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag gctcaagagc
     6541 gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg attaagatag gtaccgtcca
     6601 gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag catctttatt
     6661 gctcgcacga tacttccagg cgaaaccaac agccatcaag attgcgggaa taacgacaat
     6721 caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa tctgtccttt
     6781 gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa ataatgacaa
     6841 ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat tccacgggat
     6901 ctggtggcgt ttaaagtgtg tgggcatttt acgggaaggt tacattactg taaacatgat
     6961 taaattcctg ttacttggat ttggctgtca ggtcacatat gttgcaaaac atatcaagtt
     7021 gattttaaga agctgttgca aaagggcgtt ataacgaaag gggagtgcag aaggagtttg
     7081 gacgggcaat tggtataacc aatctggaat aaaacaattg tttaacaaat aattatcatt
     7141 atggtgacaa ttgggtgggg gaaaataaag actcat
//
LOCUS       DAZKGS010000209         7139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKGS010000209 DAZKGS010000000
VERSION     DAZKGS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            584..1573
                     /gene="araF"
                     /locus_tag="ACHOQY_004375"
     CDS             584..1573
                     /gene="araF"
                     /locus_tag="ACHOQY_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9680342.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1643..3157
                     /gene="araG"
                     /locus_tag="ACHOQY_004376"
     CDS             1643..3157
                     /gene="araG"
                     /locus_tag="ACHOQY_004376"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9680343.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3172..4158
                     /gene="araH"
                     /locus_tag="ACHOQY_004377"
     CDS             3172..4158
                     /gene="araH"
                     /locus_tag="ACHOQY_004377"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP9680344.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4325..5125
                     /gene="otsB"
                     /locus_tag="ACHOQY_004378"
     CDS             4325..5125
                     /gene="otsB"
                     /locus_tag="ACHOQY_004378"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9680345.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5100..6524
                     /gene="otsA"
                     /locus_tag="ACHOQY_004379"
     CDS             5100..6524
                     /gene="otsA"
                     /locus_tag="ACHOQY_004379"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9680346.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6531..6959)
                     /gene="uspC"
                     /locus_tag="ACHOQY_004380"
     CDS             complement(6531..6959)
                     /gene="uspC"
                     /locus_tag="ACHOQY_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9680347.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1817 a         1648 c         1839 g         1835 t
ORIGIN      
        1 tgtaactgca atgaatttta atgatagcgc tttttgtatc ttgttgatat taatcaatga
       61 atttgaattc atgatgaagt gatgatttta taaaacgttt tttcattttt gcgagatggc
      121 tctcattata cgtgttctgt taattaatga gtacagtgat aattaatggc gaaatgaatg
      181 acgtgcattt tccacatctt tgagttgcgg ttattacacc atttcaaaaa ctcaacgcca
      241 ggtaatgcgg cctattgact ggttaaaaag aagacatccc gcatgggtac caaagacaac
      301 aaggatttcc aggctaatct tatggattaa tctgctgtgc attcgacaat ttgtctgaca
      361 aattggcttt cccttatgtc ttttcccgct aaatttatgc acgttctcac tgtaattctg
      421 cgatgtgata ttgctctcct atggagaatt aatttctcgc taaaactatg tcaacacagt
      481 cacttatctt ttagttaaaa ggtaatgctt tgttttccga ttaatttaac gaatgtcatt
      541 cgtttttgcc ctacacaaaa cgacactaaa gctggagaga accatgcaca aatttactaa
      601 agccctggca gccattggtc tggcagccgt tatgtcacaa tccgctatgg cggagaacct
      661 gaagctcggt tttctggtga agcaaccgga agagccgtgg ttccagaccg aatggaagtt
      721 tgccgataaa gccgggaagg atttagggtt tgaggttatt aagattgccg tgccggatgg
      781 cgaaaaaaca ttgaacgcga tcgacagcct ggctgccagt ggcgcaaaag gtttcgttat
      841 ttgtactccg gaccccaaac tcggctctgc catcgtcgcg aaagcgcgtg gctacgatat
      901 gaaagtcatt gccgtggatg accagtttgt taacgccaaa ggtaagccaa tggataccgt
      961 tccgctggtg atgatggcgg cgactaaaat tggcgaacgt cagggccagg aactgtataa
     1021 agagatgcag aaacgtggct gggatgtcaa agaaagcgcg gtgatggcga ttaccgccaa
     1081 cgaactggat accgcccgcc gccgtactac gggatctatg gatgcgctga aagcggccgg
     1141 attcccggaa aaacaaattt atcaggtacc taccaaatct aacgacatcc cgggggcatt
     1201 tgacgctgcc aactcaatgc tggttcaaca tccggaagtt aaacattggc tgatcgtcgg
     1261 tatgaacgac agcaccgtgc tgggcggcgt acgcgcgacg gaaggtcagg gctttaaagc
     1321 ggccgatatc atcggcattg gcattaacgg tgtggatgcg gtgagcgaac tgtctaaagc
     1381 acaggcaacc ggcttctacg gttccctgct gccaagcccg gacgtacatg gctataaatc
     1441 cagcgaaatg ctttacaact gggtagcaaa agacgttgaa ccgccaaaat ttaccgaagt
     1501 taccgacgtg gtactgatca cgcgtgacaa ctttaaagaa gaactggaga aaaaaggttt
     1561 aggcggtaag taatttgccg gaaaaattcc cctctgcatg atgcagaggg ggtgtgaacg
     1621 accagtgatt cacggagacg ttatgcaaca gtctaccccg tatctctcat ttcgcggcat
     1681 cggtaaaacg tttcccggcg ttaaggcgct gacggatatt agttttgact gctatgccgg
     1741 tcaggttcat gcgttgatgg gtgaaaatgg cgcaggaaaa tcaactctct taaaaatcct
     1801 cagcggcaac tatgcgccaa ccacgggttc tgtagtgatt aatgggcagg aaatgtcctt
     1861 ttccgacacg accgcagcac ttaacgcggg tgtggcgatt atttaccagg aactacatct
     1921 cgtgccggaa atgaccgtcg cggaaaacat ctatctcggc cagctgccgc ataaaggcgg
     1981 cattgtgaat cgctcattgc tgaattatga ggcgggttta caacttaaac atcttggtat
     2041 ggatattgac ccggacacgc cgctgaaata tctctccatt ggtcagtggc agatggttga
     2101 aatcgccaag gcgctggcgc gtaacgctaa aattatcgcc tttgatgagc caaccagctc
     2161 cctttctgcc cgcgaaatcg acaatctttt ccgcgttatt cgtgaactgc gaaaagaggg
     2221 gcgggtgatc ttatacgttt ctcaccgtat ggaagaaata tttgccctca gcgatgccat
     2281 cactgtcttt aaagatggac gttatgtcaa aacctttacc gatatgcagc aggttgacca
     2341 cgacgcgctg gtgcaggcga tggtcgggcg cgacattggc gatatctacg gctggcaacc
     2401 tcgtagttat ggcgaggagc gcctgcgtct tgatgctgtg aaagcaccag gcgtgcgtac
     2461 gccaataagt ctggcggttc gcagtggtga aattgttggg ctgtttggtc tggtaggggc
     2521 ggggcgtagc gaattaatga aaggcttgtt tggcgggacg caaatcaccg ccggtcaggt
     2581 ttatatcgac caacagccga tcgatattcg taaaccgagc cacgccattg ccgcaggcat
     2641 gatgctctgc ccggaagatc gcaaagcgga aggcattatt cccgtgcact ccgttcgcga
     2701 caatatcaac atcagtgcca gacgtaaaca tgtgctcggc ggttgtgtaa tcaacaacgg
     2761 ttgggaagaa aacaatgccg atcaccacat tcgttcgctc aacatcaaaa cgccgggcgc
     2821 tgagcaactg atcatgaatc tctcaggcgg aaatcagcaa aaagccattc tgggccgctg
     2881 gttatcggaa gagatgaagg tcattttgct ggatgaacct acgcgcggca ttgatgttgg
     2941 tgctaagcat gaaatttaca acgtgattta tgcgctggcg gcgcagggtg tggcggtgct
     3001 gtttgcctcc agcgacttac ctgaagtcct cggcgttgcc gaccggattg tggtgatgcg
     3061 ggaaggtgaa atcgccggtg aattgttaca cgagcaggca gatgagcgtc aggcactgag
     3121 ccttgcgatg cctaaagtca gccaggcagt tgcctgagta aggagagtat gatgtcttct
     3181 gtttctacat cggggtctgg cgcacctaag tcgtcattca gcttcgggcg tatctgggat
     3241 cagtacggca tgctggtggt gtttgcggtg ctctttatcg cctgtgccat ttttgtccca
     3301 aattttgcca ccttcattaa tatgaaaggg ttgggcctgg caatttccat gtcggggatg
     3361 gtggcgtgtg gcatgttgtt ctgccttgct tccggtgact ttgacctttc tgtcgcctcc
     3421 gtaattgcct gtgcgggtgt caccacggcg gtggttatca acctgactga aagcctgtgg
     3481 attggcgtgg cagcggggtt gctgcttggc attctctgtg gcctggtcaa tggctttgtt
     3541 atcgccaaac tgaaaataaa tgctctgatc acaacactgg caacgatgca gattgttcga
     3601 ggtctggcgt acatcatttc agacggtaaa gcggtcggta tcgaagatga aagcttcttt
     3661 gcccttggtt acgctaactg gttcggtctg cctgcgccaa tctggctcac cgtcgcgtgt
     3721 ctgattatct ttggtttgtt gctgaataaa accacctttg gtcgtaacac cctggcgatt
     3781 ggcgggaacg aagaggctgc gcgtctggcg ggtgtaccgg ttgttcgcac caaaattatt
     3841 atctttgttc tctctggcct ggtatctgcg atagccggaa ttattctggc ttcacgtatg
     3901 actagtgggc agccaatgac gtcgattggt tatgagctta ttgttatctc cgcctgcgtt
     3961 ttaggtggcg tttctctgaa aggtggcatc ggaaaaatct catatgtggt ggcgggtatc
     4021 ttaattttag gcaccgtgga aaacgccatg aacctgctta atatttctcc tttcgcgcag
     4081 tacgtggttc gcggcttaat cctgctggca gcggtgatct tcgaccgtta caagcaaaaa
     4141 gcgaaacgca ctgtctgatg cttttttcca caacaattta acgttttttc ccaccacagc
     4201 cagccgccac aacggttggc tgttcttcat tgcaaatggc gacccccgtc acactgtcta
     4261 tacttacatg tctgtaaagc gcgttctgcg caaaacaata agaaaagaga aggaggagag
     4321 ccgggtgaca gaaccgttaa ccgaaacccc tgaactatcc gcgaaatatg cctggttttt
     4381 tgatcttgat ggaacgctgg cggaaatcaa accgcatccc gatcaggtcg tcgtgcctga
     4441 caatattctg caaggactac agctactggc aaccgcaagt gatggtgcat tggcattgat
     4501 atcagggcgc tcaatggtgg agcttgacgc actggcaaaa ccttatcgct tcccgttagc
     4561 gggcgtgcat ggggcggagc gccgtgacat caatggtaaa acacatatcg ttcatctgcc
     4621 ggatgcgatt gcgcgtgata ttagcgtgca actgcataca gtcatcgctc agtatcccgg
     4681 cgcggagctg gaggcgaaag ggatggcttt tgcgctgcat tatcgtcagg ctccgcagca
     4741 tgaagacgca ttaatgacat tagcgcaacg tattactcag atctggccac aaatggcgtt
     4801 acagcaggga aagtgtgttg tcgagatcaa accgagaggt accagtaaag gtgaggcaat
     4861 tgcagctttt atgcaggaag ctccctttat cgggcgaacg cccgtatttc tgggcgatga
     4921 tttaaccgat gaatctggct tcgcagtcgt taaccgactg ggcggaatgt cagtaaaaat
     4981 tggcacaggt gcaactcagg catcatggcg actggcgggt gtgccggatg tctggagctg
     5041 gcttgaaatg ataaccaccg cattacaaca aaaaagagaa aataacagga gtgatgacta
     5101 tgagtcgttt agtcgtagta tctaaccgga ttgcaccacc agacgagcac gccgccagtg
     5161 ccggtggcct tgccgttggc atactggggg cactgaaagc cgcaggcgga ctgtggtttg
     5221 gctggagtgg tgaaacaggg aatgaggatc agccgctaaa aaaggtgaaa aaaggtaaca
     5281 ttacgtgggc ctcttttaac ctcagcgaac aggaccttga cgaatactac aaccaattct
     5341 ccaatgccgt tctctggccc gcttttcatt atcggctcga tctggtgcaa tttcagcgtc
     5401 ctgcctggga cggctatcta cgcgtaaatg cgttgctggc agataaatta ctgccgctgt
     5461 tgcaagacga tgacattatc tggatccacg attatcacct gttgccattt gcgcatgaat
     5521 tacgcaaacg gggagtgaat aatcgcattg gtttctttct gcatattcct ttcccgacac
     5581 cggaaatctt caacgcgctg ccgacatatg acaccttgct tgaacagctt tgtgattatg
     5641 atttgctggg tttccagaca gaaaacgatc gtctggcgtt cctggattgt ctttctaacc
     5701 tgacccgcgt cacgacacgt agcgcaaaaa gccatacagc ctggggcaaa gcatttcgaa
     5761 cagaagtcta cccgatcggc attgaaccga aagaaatagc caaacaggct gccgggccac
     5821 tgccgccaaa actggcgcaa cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg
     5881 tcgaacggct ggattattcc aaaggtttgc cagagcgttt tctcgcctat gaagcgttgc
     5941 tggaaaaata tccgcagcat catggtaaaa ttcgttatac ccagattgca ccaacgtcgc
     6001 gtggtgatgt gcaagcctat caggatattc gtcatcagct cgaaaatgaa gctggacgaa
     6061 ttaatggtaa atacgggcaa ttaggctgga cgccgcttta ttatttgaat cagcattttg
     6121 accgtaaatt actgatgaaa atattccgct actctgacgt gggcttagtg acgccactgc
     6181 gtgacgggat gaacctggta gcaaaagagt atgttgctgc tcaggaccca gccaacccgg
     6241 gcgttcttgt tctttcgcaa tttgcgggag cggcaaacga gttaacgtcg gcgttaattg
     6301 ttaaccccta cgatcgtgac gaagttgcag ctgcgctgga tcgtgcattg actatgtcgc
     6361 tggcggaacg tatttcccgt catgcagaaa tgctggacgt tatcgtgaaa aacgatatta
     6421 accactggca ggagtgcttc attagcgacc taaagcagat agttccgcga agcgcggaaa
     6481 gccagcagcg cgataaagtt gctacctttc caaagcttgc gtaggagcgg ttaatctccc
     6541 gtaagtggaa ctaacagcac atcgacctca cttgaggcaa taactctttt cgccgagcag
     6601 gatgctcgcg aaaagaaact gtgattgtga ttaccgcaaa tcaccaaatc gaaatgatgt
     6661 ttgtgacata cttccagaat atgttcgctt aactcaccgt aggcaataaa cgtcttgtca
     6721 acgggatacc ccgcatcctg aattaactta tcaagaaagc tttgcgtttc ttcttgcatc
     6781 acgctgcgta aatcttcaag catcggcgcg gctaattgat tgtacatttc cgggtcggaa
     6841 gcgagagtaa ttaaactgat gtgtcccttt actggcctgg cgatagatac cgcttttgcc
     6901 agcagttgct gactttccgg tgtaaccgca acagcgacaa gaatattgct atagctcata
     6961 acctgttcct tattctgtga acttcaggtg acattaaagc tttactccta atggagaaac
     7021 gacgcaatcc caactcggtc aaacaggtaa atattagtct gattattagt aaagacaatt
     7081 aatcaatcac tcccggtgat catataattt caaatgtaat tataagaaaa tagtgtact
//
LOCUS       DAZKGS010000210         7073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKGS010000210 DAZKGS010000000
VERSION     DAZKGS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHOQY_004381"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHOQY_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP9680348.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1382..1532)
                     /locus_tag="ACHOQY_004382"
                     /pseudo
     CDS             complement(1382..1532)
                     /locus_tag="ACHOQY_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1744..2064)
                     /locus_tag="ACHOQY_004383"
                     /pseudo
     CDS             complement(1744..2064)
                     /locus_tag="ACHOQY_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(2487..3062)
                     /locus_tag="ACHOQY_004384"
     CDS             complement(2487..3062)
                     /locus_tag="ACHOQY_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9680349.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(3131..3709)
                     /gene="terD"
                     /locus_tag="ACHOQY_004385"
     CDS             complement(3131..3709)
                     /gene="terD"
                     /locus_tag="ACHOQY_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP9680350.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(3758..4798)
                     /gene="terC"
                     /locus_tag="ACHOQY_004386"
     CDS             complement(3758..4798)
                     /gene="terC"
                     /locus_tag="ACHOQY_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP9680351.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(4821..5276)
                     /locus_tag="ACHOQY_004387"
     CDS             complement(4821..5276)
                     /locus_tag="ACHOQY_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9680352.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(5299..6456)
                     /locus_tag="ACHOQY_004388"
     CDS             complement(5299..6456)
                     /locus_tag="ACHOQY_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9680353.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6456..7037)
                     /locus_tag="ACHOQY_004389"
     CDS             complement(6456..7037)
                     /locus_tag="ACHOQY_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9680354.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
BASE COUNT         1794 a         1832 c         1748 g         1699 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaatgcca atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta
      901 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt
     1081 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     1201 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     1261 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     1321 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     1381 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     1441 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     1501 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     1561 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat
     1621 aagtcccttt gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca
     1681 ccacctcgcc acgaccaaat ccaaataagt caattgcacc ttctcaatcg ccattttgtc
     1741 agtaagcgta cagcctgaac tgatggtatc ttcaccatca atgacaacgg tgatcgcaat
     1801 tttactgacg ttcgccggaa cacgatccag tgctatctca atgctggcct gctgcgaacc
     1861 ggtaacgagc ctgactgccc cctcaggaga agacaaatta ttataaaaga taaagtcaga
     1921 atcgccactg acctttccct gagcattaag catgaacagg caggtatcgg gttcgccttt
     1981 aaagccggat ttggctgggt actgaagatt cagcctgatc gtagattgct gaaggggtat
     2041 gttttgtccg gattgtaaat tcatattaac tctcctgatt tttgattatt attaatgcgc
     2101 agcgtttata tatgttccct aggcttagtt ctggacgctg gatattcggt gaggcgtaaa
     2161 tatggtatga caccattttt cataacgctg aagtttctat acctgttgaa tttgaatttt
     2221 cattgaccgg gtatcttatt ttccagggcc cgttccttca taagtcgcaa aagtaacata
     2281 tatccgaagg gcatgctgtt gatatcagac acggaatact ggctttaacc agcaaccaca
     2341 gataaacccg gggcctgcat aaaaggttca tgccagaatt aacatagctc tgctttctgc
     2401 cccccccctt tcccatattc acccgttgat agcggatcga tacccaaaaa aaaccccggc
     2461 attgccgggg tcagactcag cgtcttttag acattgacgc cgtgctggga agcaagcgct
     2521 gacagaccac cggcaaaacc ctggccaaca gctttaaact tccactcagt accatggcga
     2581 tacagttcac cgaagaccat tgcggtttcg gttgaggcgt cttcagacag atcgaaacgg
     2641 gcaatttccg tcccgttgtc gttgttgtaa acgcgcatga agctgttgct caccatgccg
     2701 aagttttgtt tacgcgcttc tgcatcatag atggtaacgg caaataccag ttttttgatg
     2761 tctgctgaga ctttggtcag atcgattttg acctgctcat cgtcgccgtc gccttcaccg
     2821 gtacggttgt cgccctggtg ctctactgcg ccatcagggc tggttttatt attgaagaaa
     2881 atgaaatggg catctgacag tactttaccg tcttcaccta ctgcgaatac ggaagcgtcc
     2941 agatcaaaac cctgaccatc ggttacacgg gcatcccagc ccaggccaac catagcgaca
     3001 ttcatggttg gtgcttcttt ggtcagagat acgttgccgc cttttacgag agaaactgcc
     3061 atttttagct cctgcaaaca gttgaatgag gctgaattaa cagccccaga tatgaaaagt
     3121 tacttttcga tcaggacgcg ttaatgccgt actgagcaca tacagatgcc agaccaccag
     3181 cataaccctg gcctactgcg cggaatttcc actcaccatt gtggcgatac agctcgccga
     3241 acagcatggc agtctcagtg gacgcatctt cggtcagatc gtagcgagcg acttcagtct
     3301 ggttatcgtc attaaccaga cgaataaacg caccggatac ctgaccaaag ctctggcgac
     3361 gagcctgagc atcgtggatg gtcacaacga agatgatctt gtcaacttca gacgggacgg
     3421 cgtccagttt aattttcagc gattcatcat caccatcgcc ctcaccggtg cggttatcgc
     3481 cggtgtgcgt tacggaaccg tcggatgacg tcaggttgtt atagaagatg aaatctgaat
     3541 cgccgcgcac tttgccgttt gaggccagca ggaatgctga agcatccagg tcaaagtcct
     3601 gaccgtctgt tgaacgcgca tcccagccaa ggcccaccag gacatttttc attgacggag
     3661 ctgctttact cagggagacg ttcccgcctt tggaaagaga aacactcata aaataacctc
     3721 ttcgattagt aattgttcag gttaacactt aaggggatta actccccttt tcctcagatt
     3781 caggtttgcc cgggaacatg acgcttgcga gaatgcccag cgccagtaca cccagtacaa
     3841 catacaggct ggttgttgcc gcgatgctgt aaccatgatg ccagatgtga tcggtcgcat
     3901 tcaggccgag ttttgccgcg atgaagaaca gcagcacgat aacggccttc tccagatgaa
     3961 ccaggtactg tttcagtgcc tcaaggacaa aatacagagt acgcaggccc aggatagcaa
     4021 acatcatggc actatagacg ataagcggtt cacgactgac ggcaatgatt gccggtaccg
     4081 agtcaaacgc gaacattacg tccgagagtt caaccacagc cacacacagg aacagcgggg
     4141 tcgcatacag cgccgctttt ttaccacggc caatggtgac atcgctgttt tctggtttcg
     4201 ccagttcagc atccacttcc ttctggttaa gtaggaaggc atgccctctg agcttcggcc
     4261 agatagggaa gaagcgttta accatacggt aagccagatg ctgggagtaa tcctcaattt
     4321 catcatcgtc atcaccgctt ttaagcatca tgaccgccgt ccaggcaacg ataatagcga
     4381 agacaacttc aacgtacggc cccagactca gcaggctcgt accgatggcg acaaagatgc
     4441 ccctgaagac gatggcacca atgatccccc agtagagaac acggtggcga taacgatccg
     4501 gaacggcgaa ccaggagaag attgccatca tgacgaacag gttatcgacc gacagcactt
     4561 tctccagcgc ataacccgtg acaaacagac tggcaacctc agcaccgtgg tggatataga
     4621 ggaaaccggc aaatgccatc gcaaccacaa cccagaatac ggaccagagc gcagcactct
     4681 tcagcgagat tggcttgtca tcacggtgca taaacaggtc aataaagatg gcaccgactg
     4741 aaagcgcaat aaaaacaatg accgtttcag tcgggaagcc gatgtgtgtg gaaaccatat
     4801 tttaccctta gggatgtgga tcagaggcca aattcagccg ggtcgaagcc caacgcgata
     4861 gcgatctcgc gggaggccag tttctcgtcc tgatcgaagt taccgtcact tttcgcaacg
     4921 gcaataccaa cacgtaaggc cagctgagcg gcctcaggct gatctttcag cgccaggatg
     4981 tatttcatgg tttcgccctt gccaatttca acatcgaaat cgaagcttga aaccagttta
     5041 ttgaagaact caatcacctc attggtatcg aagaccttca gctcttctga agagcgcaga
     5101 aagcccatca ttttctgctt ttcttccgaa cttacgccgt cactcgatac ggcaatacgg
     5161 gcacatacag caacggtgcc ctgcatgaat tttttgtttt tgaaacggcc aacctggcgg
     5221 gtcagttcgt cacggcctga gttaatggca cctttaactt tgtcgaaaaa gctcatacct
     5281 gtttcctttg ttgctgtgtt atttagaacc ggcgctccag cgaaatcccc agccaaatgc
     5341 ccggtccatt tcgtcctggc ctttgaagta ctggttaatt cgctcgactt taattgcgcc
     5401 gttttcgttt acgagtctgg caatggcgca caatgtgcga cggttctcac cttcggtcag
     5461 gcgggtctca atgggcggtt gatccggcac atgaatggtg actacaccat cagtcttgtc
     5521 ccagctggga acaccttcat aaataaaggc gtaaatcagc acttcgcgga tatgcttcca
     5581 ttcacgtcca ttgatatgca gccactcgcc gtctgataca tcacccgtcc ggtcatcgcc
     5641 cttgagctga acatacggtt catcgcgata atcaccgaat gcattaccga gcgcctgaat
     5701 gaccgattta tacccgtcct gaagctcaac aaaggccccc agatccaggt cgattccttt
     5761 ggagccgaac atacctgcaa agcctgattt gctactgccc cggtgccagt tcaggttgat
     5821 ccggatttca ccgaagttat cccgtttggt caggctgatt gcaggcttct cttttgtcag
     5881 cgataccttg ctcaggctga tttttgtctc aactggcgga ggggttacaa ccggaggagt
     5941 tggggctgcg ggagccggtt cgtcggcaat atttacgccg aaatgctctg ccagcggttt
     6001 aagtccaccg ttgaacccct gcgcaacgaa gcggaatttc cagtcattat tacgacggta
     6061 aaattccccc agaattaagg ctgcttcctg acggccgctc agttcgacac tgccgctaac
     6121 caggccggta gccccttgct ccacgtcgat ggaaagatta cgaagaccgg acaccgtctg
     6181 cccaccgtca caggtcacgg tgaacgcaat tttctggaca tcaggtttca gccggttgag
     6241 cgcgacagta aaggttgagt actggccttc actgaccagg ctgacggtac catcatcgtt
     6301 acgtggctga ccataaaaca ccatgtccgc atcaccctgc accttcccgt cggcatacag
     6361 gcgaaacgcc gaagcatcca cctggccgcc cgaggtgatc cggacccgca actcctgggc
     6421 tggaacgggg gcattgcccc ccggcgtcag gttcattagc gaataaccgc tgaaacgata
     6481 tcggagtgca tatcgtcaat tgtgcggccg cgacaggcat ggccaagggc ggtaaagtcc
     6541 cagttgccgt tattacggcg cagcgaagaa ataacgatcc cggtatgtga gccctgctca
     6601 gtcagcttgt agcgagccag ctctttgccg gtttgatcga caacgcggca gaaagcgttt
     6661 tcgacgtcat tgaacgactg accacggaag ctgtttaccg taaacgccag gtattcaacg
     6721 tttgcaggca ggcgggagag gttgacctta atcacttcat cgtcaccgtc accctcaccg
     6781 gtcaggttat cgccactgtg cacaacagcg ccgcaggtgg atttcagttt gcggaaccag
     6841 atggtgtcaa ttgttttacc tgtgctgtcc attaatacac agccagcatc gagatcgatt
     6901 gagtcgttgc ctccaaacaa tcctccaaga agaccttttt tcttaaccgg atcccaaccg
     6961 agaccgaaat gaagctggct taatgcagat gattctttgc tgagtgatac cgtctggttc
     7021 ttaactaatg aaaccatgtt ttattcctta aaatggtaat gaccgagaca atc
//
LOCUS       DAZKGS010000211         6914 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKGS010000211 DAZKGS010000000
VERSION     DAZKGS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..5963
                     /gene="efa1"
                     /locus_tag="ACHOQY_004390"
     CDS             <1..5963
                     /gene="efa1"
                     /locus_tag="ACHOQY_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFP9680355.1"
                     /translation="VEILNKLSELPPHELLTPVLGQSVNPLGMGYSSDNGKITEQVIV
                     SGADGFDNPISGLIYTYLEDLYNIHVRMREGTLNSQNLRQLLENSVSSCFLTEQSINK
                     LLSEAEKRPYQSLTEIHQHLTGLPTIADATLSLLSVGLPGTGKLLRREQDYGRPPVTA
                     IQDSTFVLPYNFKGIGFNDNIISSAPVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFE
                     MAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYLQTEDTAHYSILQENLMTVSALHQTS
                     NRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYITNESLLHKAAWNQERITLLFNEKGV
                     KRALISTPNHTLVLQQLEDIYRLTDPNFGHADFLSPIDALKFIEAMIQLTPTLQEYYG
                     LLNKDINKHIQVHYAESDMVWNKLLPENDAGLSTRIQHTTTDRLANLAEPVAVAGISL
                     PVKTLYDIGATLDGRRITSPPTSEQIPSLRLNGDVLNDYLSRTVLTPEQADNIRKILH
                     TQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRS
                     SGRHLSVENIELADIGSGRFNLQIRDGETLHTTSVEVPEVVSRFQKLSTMLSALPASG
                     IMDFDLGMSVVGVVQYARLLQQGHEDSTLAKINLAMDIKQLSEATLGSMIQIAGNKFL
                     NTEGIQGFRLESAVAEGMRSVATRTGGTMGKALSASARVLELPVLETVLGTWNLYNSV
                     IQLQQATSYSETMAARVQIAFDSISLGLTAASVAFPPLIIATGPIAAIGMGASSIARN
                     VARKEERHTQWLEYKKFLTDGSKHIVVASPERGLLDFSGNKVFGKMVLDLRQSPPLLH
                     GESSFNADRKIGHRPDLGDWQIREKVGYANSISPYSSLAHGYANSKWPQTIPKIPSGE
                     YDTIILGYGHQYQANTEIEYLSNWIVWREAVPDSTSRHKRPPLEVLNSQCTVIAGERK
                     TTVLPLRVLSDLTPECTEQAISLKDYKFILRGGSGGLAVQVGGAGYYDIDANPVAKEN
                     TLSFRGLPEEFPLAFDLSKQTQSVMLKTTDGEVPVMTITQKGINTLVGTTAGENWLTG
                     NDKDNTFHTSSGGGTVISGGGNNRYIIPRDLKTPLTLTLSNSSVSHEILLPETTLTEL
                     KPVAFELSLIYWAGNNIKVQLEDETQLNRFAGNFRGHTSDGITLEAVSQENGIQLGVS
                     SCDVQRWQAVYPEENNRPDAILDRLHDMGWSLAPEVRFRGGETSASYDPLNRQLVYRL
                     QERYSEFRLTGSRHYTTAVTGTPGSRYIITGPDTAQISPAQIILAGDNDHPETIDLLE
                     ASPVLVEGKKDKNSVILTIATIQYSLQLTISGIDESLPGTTRVAIQPQDTRLLGDVLR
                     ILPDNGNWVGIFRSGHTPTVNRLENLMALNQVMTFLPRVSGSAEQVLCLENLGGVRKK
                     VEGELLSGKLKGAWKAEGEPTVPVNISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPL
                     KITSAGEMQLSERQWQQQEHIIVKPDNEAPSLILSEFRRFTISSDKKFSLKLMCHQGM
                     VRIDRRSLSVRLFYLREQPGIGSLRLTFRDFFTEVMDTTDREILEKELRPILIGDTHR
                     FINAAYKNHLNIQLGDGVLNLADIVAEYARIQKEETSKILYQYQGAMKKKTDGPSVVE
                     DAIMTTTVTTDSGEPFPTFHPWYTDDLSGRYKSVPMARKADTLYHLTPKGDLQIIYQV
                     ATKMVNQAMIVSLPNYRHEWEKYNLSILSEIPQNNNTVVHSILRVNGPTMQVRTIDYR
                     GTDENNPIVSFSDTTFINGEQMLSYDSHSSGRVYSREEYMMWELQQRVSEASSARTQD
                     YWLMDAAVRNGEWKITPELLRHTPGYIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLT
                     TIQNNVFSRQGGGYQVYYRIDGMAGADIADNAPGETRCTLRPGTCFEVTSVDERHYEW
                     NIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6135..6485
                     /locus_tag="ACHOQY_004391"
                     /pseudo
     CDS             6135..6485
                     /locus_tag="ACHOQY_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2042 a         1578 c         1569 g         1725 t
ORIGIN      
        1 ctgttgaaat tttgaacaaa ctttcagagt taccaccaca tgaactactc acacctgttc
       61 ttggccagag tgttaatcct ctgggcatgg gctactcatc cgataatgga aaaatcacag
      121 agcaagtaat agtcagtgga gctgatggat ttgataatcc catatctgga cttatatata
      181 cctatcttga agatctatat aacatccatg taaggatgcg agaaggtaca ctaaattcac
      241 agaatcttcg tcagcttctg gaaaactctg tttcttcatg ctttttgact gagcaaagta
      301 ttaataaatt acttagtgag gcagaaaaaa ggccttatca gtctttaaca gaaatacatc
      361 agcatctcac aggattacca actattgccg atgcaaccct ttcattactt tctgttggat
      421 tacctggtac gggaaaacta ttgcgcaggg agcaggacta tgggcgtcca ccagttacag
      481 caattcagga ttccacattt gtactccctt ataatttcaa aggtattggt tttaacgata
      541 acattatatc ctctgcacct gtagtctcct cattacattt tatcgctgaa catgcgaaat
      601 atactttgtt gtcatggcct gagttttatc gtcatcatgc acagcgatgg ttcgaaatgg
      661 ctaaaggata tggaagccag aatattgatt ttcaccctca gtctctattg gtaacccaag
      721 aaggacgctg tatgggatta gccttacttt atttacagac tgaagatact gctcattata
      781 gcattctcca ggaaaaccta atgactgtga gtgcacttca tcagaccagt aatcgcgata
      841 agttgccact gtccaaagat gataattcct taatgacaag aacttatagt ctgattgaaa
      901 tgctacagta tcagggaaac aaatatatta ccaacgaatc gctactacat aaggccgcat
      961 ggaaccaaga aagaataact ttattattca atgaaaaagg agttaagcga gccctgataa
     1021 gcacgcctaa tcatactctg gttctgcaac aactggagga tatttaccgg ctcactgatc
     1081 caaattttgg gcatgcagat ttcctttcac ctatagatgc tctgaagttt attgaggcta
     1141 tgatacaatt aactccaaca cttcaggaat attatggcct attaaacaaa gacattaata
     1201 aacatataca agtacattat gccgaatcag atatggtctg gaataagctt ctgccagaaa
     1261 atgatgctgg actgagcacc agaattcagc acaccaccac cgaccgtctg gcgaatctgg
     1321 ctgaaccagt cgctgttgca ggtatctccc tgccagtaaa aacactttat gatatcggag
     1381 ccacccttga cggtcggcgc atcacctctc ctccaacatc ggagcaaatc ccttctctgc
     1441 gtctcaacgg tgatgttctg aatgattatc tgtcccgcac agttctgact ccagaacagg
     1501 ctgataacat aagaaaaata ctgcacactc agggaatacg cagcggtacc cgtcccatag
     1561 atccggagat gattcgtggg acgcaggatg acctagtttc gtcacagact cgtctgcaaa
     1621 ggcaagcaac acgggttaaa cagcaactcg ccggcgtact tgatactctg caacagcact
     1681 tccagaacat tccacgttca tccggtcgtc atctttctgt agagaatatt gagctggctg
     1741 atatcggaag tgggcgtttc aaccttcaaa ttcgagatgg agaaacattg cataccactt
     1801 ctgtggaagt accggaagtt gtgtcccgtt ttcagaaact ttctaccatg ctttcagccc
     1861 tgcctgccag tggaatcatg gatttcgacc tcggcatgag tgtggtcgga gtcgtccagt
     1921 atgcccgcct gctacagcag gggcacgaag acagtaccct agccaagata aatctagcta
     1981 tggatatcaa gcaactttcc gaagcaactc tcggcagcat gattcagatt gccgggaata
     2041 agtttctcaa tacagaagga atccaggggt tcagactgga aagcgccgtc gctgaaggta
     2101 tgcgctcagt agcaacccgt accggaggca caatggggaa agccctttct gccagtgccc
     2161 gtgttcttga gctgcctgta ctggaaacag ttctggggac atggaacctg tacaacagcg
     2221 tcattcagct ccagcaagct acatcttatt ctgagacaat ggctgcccgg gtacagattg
     2281 cgtttgattc catttctctg ggattaactg ccgcttcggt agctttcccg ccactaatta
     2341 ttgccactgg ccccattgcg gctattggta tgggagcttc cagtattgca cgtaatgtgg
     2401 cacggaaaga agaacggcat acacaatggc tggaatataa aaaattcctg actgatggca
     2461 gtaaacacat tgttgtggcc tctccggaaa gaggtctgct ggatttctcc ggaaacaaag
     2521 tttttggaaa aatggtgctg gatctgcgtc agtctcctcc tctcttgcat ggagaaagct
     2581 cttttaacgc tgaccgcaaa atcggtcatc gtccggatct gggggactgg caaattcgtg
     2641 agaaggtggg gtatgccaac agtatcagtc cctactcttc cctggcgcac ggttacgcca
     2701 acagtaaatg gccacaaaca ataccgaaaa ttccctcggg agaatatgac accataattc
     2761 tgggctacgg tcaccagtat caggccaata cggaaataga atatctgtct aactggattg
     2821 tatggcggga agccgtacca gacagtactt cccgccacaa acgtcctcct ctggaggttc
     2881 ttaatagtca gtgtactgtg atagctggag agcgtaaaac cacagtactt cccctgagag
     2941 tgctcagcga tctgacaccg gaatgcacag aacaggctat atcgttaaaa gattataaat
     3001 tcatactgag agggggaagc ggtgggctgg ctgttcaggt cggtggcgcg ggatattatg
     3061 atattgatgc aaatcctgtg gcaaaagaaa atacgctctc ttttcgcggg ctaccggaag
     3121 agtttccgct cgcctttgat ttatcaaaac aaacacagtc ggtcatgctg aaaacaacgg
     3181 acggtgaggt gccggtaatg accatcaccc agaagggaat aaacaccctg gtcggtacaa
     3241 cggccggcga aaactggcta acgggtaacg ataaggacaa taccttccat acgagttctg
     3301 gtggcggtac tgtcatctct ggtggaggga ataaccgcta tattatcccc cgcgatttaa
     3361 aaactccgct gacactgacg ctgtctaata gctcggtctc tcacgagatc cttctgccag
     3421 aaacaactct gacagaatta aaacctgtcg cctttgagct gagtctgatt tactgggccg
     3481 ggaacaatat aaaggttcag ctggaggatg agacacaact gaaccgtttt gccggaaact
     3541 tcagggggca taccagtgat ggcattactc tggaggcggt ttcccaggaa aatggtattc
     3601 aactgggggt ttcatcatgt gatgttcaac gctggcaggc tgtttatccg gaagaaaaca
     3661 acagaccaga cgccatactg gacaggctgc atgatatggg atggagcctg gcccccgagg
     3721 tcaggttccg gggaggagaa acatctgcca gctatgaccc cctgaaccgt cagctcgttt
     3781 accggcttca ggagcgttac tcggaattca ggctgaccgg cagtcgccat tacaccacgg
     3841 ctgtaaccgg aacccccgga agccggtaca ttattacggg gccggataca gcacagatat
     3901 caccggcaca aatcatactg gccggtgata atgaccatcc ggaaacgatt gatttgcttg
     3961 aggccagtcc tgttctggtt gaagggaaaa aagacaaaaa cagcgtgata ttaacgattg
     4021 ctacgattca gtattccctt caactgacaa tatccgggat cgacgaatcg ctgcccggga
     4081 caacccgtgt ggcaattcag cctcaggata cccgtttact gggtgacgta ctccggatct
     4141 taccagataa tggtaactgg gtggggattt tccggagtgg tcatacacca acggtaaacc
     4201 ggctggaaaa tttgatggca ctgaatcagg taatgacgtt cctgccccgg gtatccggaa
     4261 gtgcagagca ggtattatgc ctcgaaaacc taggtggcgt aaggaaaaaa gtggaggggg
     4321 agttactgtc agggaagctg aaaggtgcgt ggaaagccga aggtgaacct actgttccgg
     4381 taaatatctc agatctaagt atcccaccct attcacgtct gtatctgatt tttgaaggga
     4441 aaaataatgt gttgctacgc agtaaagtac atgcagctcc gttgaaaata acatccgcag
     4501 gagagatgca gttatctgaa aggcagtggc aacagcagga acatattatt gtcaagcccg
     4561 acaacgaagc cccctcatta atactcagtg aatttcgtcg tttcactatt tcatcggata
     4621 aaaaattttc tttaaaactg atgtgccatc aggggatggt ccgcatcgac cgcagatcat
     4681 tatcggtcag attgttctat ctgcgtgaac agccaggtat cggcagttta cgtctgacgt
     4741 tcagagattt tttcacagaa gtgatggata caacggacag ggaaattctg gagaaagagc
     4801 tgagaccaat tctgatagga gatacacacc gctttatcaa cgctgcatac aaaaatcatc
     4861 tgaatatcca gttaggagat ggcgttctga atctggcaga cattgttgcg gaatacgccc
     4921 gtattcaaaa ggaagaaaca tcaaaaatat tgtatcaata tcaaggtgcc atgaaaaaaa
     4981 aaacagatgg accatctgtg gtagaagatg ccattatgac cactactgtc acaacagatt
     5041 caggtgaacc attccctacc ttccacccgt ggtatacaga tgatttatca gggcgttata
     5101 agagcgtacc tatggcaaga aaagcagata ctttgtatca cctgacacca aaaggtgatc
     5161 tacagataat atatcaggta gctacaaaaa tggtgaatca ggcgatgatt gtatccctgc
     5221 caaactaccg acacgagtgg gaaaaatata atttaagcat cttatccgaa atccctcaga
     5281 acaataatac tgttgtacat tcaatcctca gggttaatgg ccccacaatg caggtgcgca
     5341 caattgacta cagaggaacg gatgaaaaca atcccatagt atctttttca gatacaacct
     5401 tcatcaatgg tgaacagatg ttgagttatg actcgcattc atcagggcga gtctattcca
     5461 gagaagaata tatgatgtgg gaattgcagc aacgggtatc agaagcttcc agtgcccgga
     5521 cacaggatta ctggctgatg gatgcagcgg taagaaacgg agaatggaag atcacaccag
     5581 aattattacg tcacacaccg ggatatatcc ggagtacggt atcgaaatgg tccagaggat
     5641 ggctgaaaac cggcacaata ctccagactc cagaagacag aaatacggat gtatacctga
     5701 ctaccataca gaacaatgta tttagtcgtc aggggggcgg ctaccaagtg tattatcgga
     5761 ttgatggaat ggctggtgcg gatatagcgg ataatgcacc aggggaaacc cgctgcaccc
     5821 tcaggcccgg aacatgtttt gaagtgacaa gtgtggatga aaggcattat gagtggaata
     5881 tcatttatgt cacgctgaaa acctgtggct ggagccgaaa tggccaaagc aaaacgccga
     5941 atggtgacaa cctttttaac taattaaaat ctctgttaaa gatgtataac ccggcatcta
     6001 tgtgctcacc agcatataga tgcaatgtga ttcccctgat ggtcgtatca gagatggtat
     6061 gcgagcctgt acatagattt gtgtaattgc ctgattttga tatgttcaat ccaacatcaa
     6121 aagcaggtta atttatggac gaaaaacagt tgcaggctct ggctaacgaa caggccaaaa
     6181 atctcaaaac ccctgaagat ctcagccagt tcgatcgcct gctgaagaaa atcagcgttg
     6241 aggcagctct caacgctgaa atgtcccacc atctgggcta cgataaaaac cagcctaaac
     6301 cgggtgccaa ctcccgcaat ggctattcca caaagaccgt tatcaccggt gatggccccc
     6361 tggaactacg cactccgcgc gatcgtgatg gctcttttga accgtaactt gtgaagaaaa
     6421 accagacccg gatcaccggg atggataacc agatcctgtc gttgtacgcc aaaggcctga
     6481 caaccgtaag cgtacagcct gaaccgtctg gtcagaatct gacgaattag agtaagctgg
     6541 acatccccag atatattatg gctgccagcg tcatatcggc ataaatagag atatccctta
     6601 acgctaacca gaaaaaaata cctataagag cctgtacata gagttgtgtc gttgcctgat
     6661 gttgatatgt caaatccaac atcaaatagc ggtaaattta tggataaaaa attacaggct
     6721 ctggttaacg aaacaccccc ctcttgctct attcccctct ttcatcattt attacaatat
     6781 ttatttcaat aaaaaccgat attaccaatc acatttatca caatgcaact taatatcggt
     6841 ttttatcttt taactctcta aacaatcaac agtaattaat aattttaaat ctacttttta
     6901 tgcctttttc gaca
//
LOCUS       DAZKGS010000212         6720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKGS010000212 DAZKGS010000000
VERSION     DAZKGS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHOQY_004392"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHOQY_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9680356.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHOQY_004393"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHOQY_004393"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9680357.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHOQY_004394"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHOQY_004394"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9680358.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHOQY_004395"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHOQY_004395"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9680359.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..>6720
                     /gene="fdhD"
                     /locus_tag="ACHOQY_004396"
     CDS             5895..>6720
                     /gene="fdhD"
                     /locus_tag="ACHOQY_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9680360.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRL"
BASE COUNT         1461 a         1828 c         1827 g         1604 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
//
LOCUS       DAZKGS010000213         6715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKGS010000213 DAZKGS010000000
VERSION     DAZKGS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..278
                     /locus_tag="ACHOQY_004397"
     CDS             <1..278
                     /locus_tag="ACHOQY_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFP9680361.1"
                     /translation="KKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDS
                     EGVDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
     gene            complement(506..664)
                     /locus_tag="ACHOQY_004398"
     CDS             complement(506..664)
                     /locus_tag="ACHOQY_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9680362.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            complement(1324..1515)
                     /locus_tag="ACHOQY_004399"
     CDS             complement(1324..1515)
                     /locus_tag="ACHOQY_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP9680363.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGEDL"
     gene            1580..1903
                     /locus_tag="ACHOQY_004400"
     CDS             1580..1903
                     /locus_tag="ACHOQY_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9680364.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQG
                     ARDDC"
     gene            2112..2543
                     /locus_tag="ACHOQY_004401"
                     /pseudo
     CDS             2112..2543
                     /locus_tag="ACHOQY_004401"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2581..3099
                     /locus_tag="ACHOQY_004402"
     CDS             2581..3099
                     /locus_tag="ACHOQY_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFP9680365.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSN
                     ETKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVT
                     HYQGKDWIQLTE"
     gene            3106..3339
                     /locus_tag="ACHOQY_004403"
     CDS             3106..3339
                     /locus_tag="ACHOQY_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFP9680366.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENWR"
     gene            complement(3714..3920)
                     /locus_tag="ACHOQY_004404"
     CDS             complement(3714..3920)
                     /locus_tag="ACHOQY_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680367.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQ
                     SGDGKLMATYRRNKKAIDKLDAEG"
     gene            complement(3913..4296)
                     /locus_tag="ACHOQY_004405"
     CDS             complement(3913..4296)
                     /locus_tag="ACHOQY_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680368.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKEL
                     ISKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINH
                     KISDTEDAVLSVGKIINLIEDSQHG"
     gene            complement(4687..5748)
                     /locus_tag="ACHOQY_004406"
     CDS             complement(4687..5748)
                     /locus_tag="ACHOQY_004406"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="HFP9680369.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMN
                     PIYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVI
                     KGNNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHE
                     IRKIFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKS
                     PILDQNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNI
                     NRIYTEMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPE
                     RRFDQSVYYTASHLFDEIK"
     gene            5836..>6715
                     /locus_tag="ACHOQY_004407"
     CDS             5836..>6715
                     /locus_tag="ACHOQY_004407"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP9680370.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSH
                     PIKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKR
                     IKPKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGK
                     LDVRDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTV
                     RGVNRPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGN
                     AVPVELAKYVAVTIMEY"
BASE COUNT         1768 a         1310 c         1751 g         1886 t
ORIGIN      
        1 aaaaaaaaca agtaaaaggt atttatgata aagaaggctt tagggcgtgg ttgctgaatg
       61 aaaagaaact aacaaaaaga acctctagcg atattatatc tagatgttgc agaggtgttt
      121 cattttttga ttcagagggg gtggattttt ataactgtga aatagatgaa attattatga
      181 aactggaaag gcttgaatcg tttgtcaggc ttggggttag cctgaagtct cagttaagaa
      241 gggcttttaa attatattac gaatattgtc gaaggtaata aatacgaatt tatgtctctc
      301 aggggagggg gaggggataa aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc
      361 gtaatctgct gcctgtaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa
      421 tctagagcta ccaactcttt cttccgaagg taactggctt ggtggagcgc agataccaaa
      481 tactgtcctt ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa
      541 tccaccttgt ccagttacca gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt
      601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc
      721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg
      901 ggcggagcct atggaaaaac gccagcggcg cggccttttg ttgtcctttg cctttttgct
      961 cacatgttct ttccggcctt atcccctgat tctgtggata accgtattac cgcctttgag
     1021 tgagccgact ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     1081 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     1141 atatggtgct cttttcttat aatccgcact gatgccacat agttaagcca gtatacactc
     1201 cgctatcgct acgtgactgg ttcagggctt cgccccgaaa ccccctgacg cgccctgacg
     1261 ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg ggagctgcat
     1321 gtgtcagagg tcttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     1381 ggcgtggtcg tgaagagatt cacaaatatc ggcctgttca tctgcgtcca gttcgttgag
     1441 tttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     1501 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     1561 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     1621 actggaacgc tgtgagggta aacaactggc ggtatggatg cggcgggtct gcctgggtga
     1681 gccggttgcc cgttccggga agctgccgac actggcaccg ccgttactgc gtcagctggc
     1741 cgccatcggg aataacctga atcagacagc gaggaaggta aacagcgggc agtggtcttc
     1801 cggtgaccgg gttcaggtgg tggccgcact gatggccatc gagggagagt tgcgcagctt
     1861 acggcaggtc gtcagggagc agggagcgcg ggatgattgt taaatttcat gccaggggaa
     1921 aaggcggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     1981 caacggtgct tcagggtaat ccggaagaag tcagggaact catcgatgcc acgccatttg
     2041 cgaagaaata cacgtccggt gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac
     2101 gtgaaaaagt gatggagagc tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt
     2161 acagcattct gtgggtggag caccaggaca aggggcgtct tgagctgaat tttgtcattc
     2221 cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccga gcagacaggc
     2281 cgagaattga tgcctggcag acactggtga atcaccatta cgggctgcat gaccccaacg
     2341 ccccggagaa ccgccggata ctgactctct ctgataacct gccggaaacg aaacaggcgc
     2401 ttgctgagag cgtcacacgc gggatagacg ctctttatca tgtaggggag ataaaaggcc
     2461 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa
     2521 gcagcatcag cattgcagac ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt
     2581 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa
     2641 tctacagaga aaatgctgaa cgacgagttc aggaagctcg gcgagtctgt cagcgaggct
     2701 gtgacctcaa acgagacgaa aatcagagac gctatagccc tgttcacagc ctcgacagag
     2761 aaatcgctgg aaaaacacag ggaaggggtg aaagaggcga tgatgcagca caggaaggac
     2821 gtgttaaagc tggcagggaa tacgggcatg atgttactgg ggatagtctt tctcctgttt
     2881 accgcgagtg gcgggacgct ctggtatctt ggcgggagga tacaggcgaa cctggaagaa
     2941 atcaggaagc aggaagagac attgcagaaa ctgaacgcga agacatgggg cgtggagttt
     3001 gtgcaggacg ggagaaggaa atttctggtc attccgcaag ggatgtcagc agaggtgacg
     3061 cactatcagg ggaaggactg gatacaactg acggagtgat acggaatgac agagctggaa
     3121 acacatttgc tgaacgcctt agagcagctg caacaggact atatgcagcg gctgaacgaa
     3181 tgggagagcg ccttcgtgga attgcagaag atgttttcgc ttacgcaacg ggacaacgcg
     3241 atgctgaacg agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgagcggaca
     3301 gaacgcttga gccagttata cagcgagaac tggagataag agaggaacgg ctgatacagg
     3361 agcgcgaaca tgcgttatcc ctggaacggg agcgtcagcc ggaaatacag gaacgcatac
     3421 tggatggtcc ttcgttgtcg ctgtgatggc gaaactataa aaaatggcag gttcagtgga
     3481 ttttgacgga ctaatgtagt ctgcaccatc tggttgcata ggtattcatg cggttaaaat
     3541 ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct
     3601 gccgtgatcg caccgaacgc gttttagcgg tgtgcgtaac ggatgttatg gtaaatacaa
     3661 tatgcagcca gtcttagaga cgaaaagtcc gcccagaaga ctgacttttc agatcaacct
     3721 tcagcatcta acttgtctat cgctttttta ttgcgccgat aggtagccat tagtttacca
     3781 tcgcctgatt gtaagtcacg acctgcttgc tcagcatagg tatcgtttac agtcttagcg
     3841 ccttttttat aggccttttt aacatcatca aatccggctt ggatgacttc tttcggcttt
     3901 tcttgaggta atttagccat gctgtgaatc ctctataagg ttgattattt ttccaacaga
     3961 taagacggca tcttctgtgt cgctgatttt atgatttatc caaccgtcct ctatctttaa
     4021 tttgttcact actcctaatg taaaaacaat ctttttttca ggaaagaaaa ttgcaaaggt
     4081 tctacttttc tctttccaat cagagggcgg cgtcgaacgg aacgctacat tcctcgattt
     4141 aagtatcgct ttgattttac taattaattc ttttttttgt ttattgctga gatttttgac
     4201 gaaccctttg ctgagattgt ctgtgtaatc atcaaaagaa atatcaactg gtttgttttt
     4261 ttgtttttta tcagatagat gaacaatgtt tgacactgga tatcctgctt tttgatgaat
     4321 gaatactcag aagagtagcg gaactgtctt gttagtcaca gaggtagagt atcaattcta
     4381 cgatagatgg gttcgaagat ccggctgaga ccgttcagta cgcggtcgag atgttatgtt
     4441 caatgtaatg accgtcgaaa ttgcctttac gggcatgaag caaccaggag caatcattgg
     4501 caaaggtaac tagagttcaa tgcatggtaa tcatttttga gcttgtgatg catagagttg
     4561 gttgctgttc cattgcgtct caaactccgt caagatgctc gccgtaaaag cgagcatgtc
     4621 tagtatgcct gttccgctac tgatttttga tgggcgaatt cagggctggc gagccaccaa
     4681 gaaaaattac tttatttcat caaacaggtg agatgcagta taataaacgc tttgatcaaa
     4741 gcgtctctct gggcttaatt tattttgccc gccatttaaa attatttctt ttaatactga
     4801 tggtgggagg atgggttttg ttcttcttgg gtgatccata taagcttttg ttatttttcc
     4861 gtttagcgag aactctttat cactttgata tccaaagttc atctcagtat aaattctatt
     4921 gatgtttgtc ttgtttttgc tcgcaatttc atacaccatt ctggcgagtc tggctgatga
     4981 tctcattagt ctgctggcag atgaattgtt atatgaggat ctatcaataa aggttcgaca
     5041 caaagcaaaa tcactccaga tgaatatgtc aaaggcattt tgatctaata ttgggctctt
     5101 gccttttgtt ttccataatg gttgcattaa aaaaggtttc tggtaatcaa gaaaattttc
     5161 ttggaaagtg tttaggcaat ttaatatatt ttctttcttt gaaagaactt cagtttcatt
     5221 cccccagtgc tgtatgcttt cgcatgtgga ttcaaatatt tttcttattt catggaattt
     5281 atcgctgcaa gagtgcatta tgccaagggc acaataagaa gtgcttgcag gtcttattac
     5341 aatctctgaa ctccattcgc tctcgttttc tttatatgtc gtgctatctg gaagtacggt
     5401 taattttatt tctagtgctt ttaaaaaact attgtttcct tttataacta gatctattcc
     5461 ttttatgtca tcataagcat actgttggta tggttcatat tttttctcaa attgaaattc
     5521 aagatcacta ttttttaatg ttgtgttaaa aacgaagtca aaagatactt ctttggcatg
     5581 cacttttaaa tccttatcta aataaagata aatcggattc attttgttgt ctctcatata
     5641 acatgctaga gaggtgggaa aagaactgtt aaattggttc tttccccaca gatcgctctc
     5701 tttcctgttg gtgtttttta aaccataaag agattctatt ttgctcactt tttgctcctg
     5761 acgtcaattt attgacgtct atggtgtgtt tctatatcat gatgtggagt gatatgataa
     5821 tgagtttttt atgttatgag aataattgat ctttttagtg ggtgtggggg gttgtcccta
     5881 ggctttttga agggggggtt tgatgttgta ggtgcttatg atttctggga tcctgctatt
     5941 gaatgttaca gagataattt tagccatccg ataaaaaaac tagatttgag caatgttgat
     6001 gatgtggtga gggagcttaa agatatagac ttcgatatga ttattggagg gcctccttgc
     6061 caagacttca gtcatgcagg tttaagaatt gaaggtgcta gagctaactt aactcgctca
     6121 ttttcagaaa ttataaaaag aataaagcca aagtggtttg tgatggaaaa tgtagatcgg
     6181 gcattaagaa gtggtgccta tttagaagct cgaggaatct ttaaagaatc agggtatggg
     6241 ttaacagaaa ttgttttgga tgccagtaag tgtggtgttc cgcaaaaaag aaaaagatta
     6301 tttgtaattg gaaagcttga cgtgagggat ggttttatcc ttaacgaagt gatgtgcggg
     6361 atatcgaaag atagtatgac agttagaaat tatcttggtg attcattggg gattgagtat
     6421 tattacagac atccgagaaa ttataacaga agggctattt tctctattga tgaacctgcg
     6481 ccaacagtaa gaggggttaa tagaccgata cctgatggat atttagggca tgcaggcgat
     6541 cctgtcagta tttctgagaa tgtaaggcca ttaacaacct ttgaaagagc aagattgcaa
     6601 acatttcctg aagactttaa atttaaaggt gcaaaaacaa atttggagca aatgataggt
     6661 aatgctgttc cggttgagtt agcaaaatat gttgcggtga caatcatgga gtatg
//
LOCUS       DAZKGS010000214         6713 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKGS010000214 DAZKGS010000000
VERSION     DAZKGS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..178
                     /locus_tag="ACHOQY_004408"
     ncRNA           42..178
                     /ncRNA_class="other"
                     /locus_tag="ACHOQY_004408"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(269..955)
                     /locus_tag="ACHOQY_004409"
     CDS             complement(269..955)
                     /locus_tag="ACHOQY_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFP9680371.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1536..2324)
                     /gene="phoH"
                     /locus_tag="ACHOQY_004410"
     CDS             complement(1536..2324)
                     /gene="phoH"
                     /locus_tag="ACHOQY_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9680372.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2943..4214)
                     /gene="efeB"
                     /locus_tag="ACHOQY_004411"
     CDS             complement(2943..4214)
                     /gene="efeB"
                     /locus_tag="ACHOQY_004411"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9680373.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(4220..5347)
                     /gene="efeO"
                     /locus_tag="ACHOQY_004412"
     CDS             complement(4220..5347)
                     /gene="efeO"
                     /locus_tag="ACHOQY_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9680374.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5405..6235)
                     /gene="efeU"
                     /locus_tag="ACHOQY_004413"
     CDS             complement(5405..6235)
                     /gene="efeU"
                     /locus_tag="ACHOQY_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9680375.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1737 a         1794 c         1544 g         1638 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaagtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg gttcgacaac tggcgacagc cagttggaca gaccgtgaac
      121 gcagtgagcg ggctgcccgc agggcgagcg aagcgagtca atccccccct caccgccatc
      181 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
      241 gatgagaagc cccgaccggg atgcttagtt aatgcaccgg ttcactcttt gtcgccgcga
      301 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      361 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      421 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      481 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      541 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      601 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      661 acattttttc acaacgcgtg attaaccact cttttctttc caataaaata ccttcggcaa
      721 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      781 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      841 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      901 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      961 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
     1021 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
     1081 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
     1141 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1201 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1261 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1321 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1381 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1441 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1501 gcccggttcg cccgggctct gcaccgataa tacacttaac tgtaggcatg cagcgtacgt
     1561 tggcaaagtg ccgaacgtac gcagtcctct ttaccgaacc ggacgatccc aaccatttca
     1621 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1681 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1741 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1801 aaggtacgtc cacgcatata ggcgaacggc gcaatttcca ccttccctat ttccggtcgc
     1861 aggcagtact gcataaagga agcccctaag cgccggacca gtacgtcgta gaccgggcga
     1921 aaatagggag caaacttttc tgcgatatct ccaggtaaga agccaagatc ttcatcggct
     1981 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcttctgcc
     2041 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     2101 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     2161 gaagtatcgc gactgtcgcg ggccatacca atggcttcta cgccgcccat ctgcacaagc
     2221 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2281 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2341 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2401 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcaca
     2461 ccgtttgcgg cgtcggtaac acgagtaaag agaggaagtg agaaaaatac agagtgacga
     2521 agttgctacc ggaggagaaa tctccgcaag tggtggagcg tagcttaaca agacattgtc
     2581 cagaacagga cgttaccatc cgcgatctcc atagtgactg actatcactg ccgggaactt
     2641 ccgctgctac ttaataatta caacagatct cgcattcatt gcaacaatat attcacttat
     2701 atttaaccat aaaacaccat ttcagtgaca ttagtttcta ctggaaagat gacagagtga
     2761 tgacagatat gaaaaaagct gtgtgctttc agcaggattc gcatcaggca tctgcgcacg
     2821 aacgccggat gcggcgtgaa cgccttatcc gcctaccgaa cccgcaccga ttgtaggcat
     2881 aagaagacgc gcaagcatcg catcaggcat ctgcgcaaga acgccttatc cgtctataaa
     2941 cattaaaccc gcaataacgc gcttccgaga taatcgttcg cgtccttcac tcccggcagc
     3001 gcaaaaaaat aaccgccgcc gataggttta acgtattcct ccagcgcttc gccattgagc
     3061 cttttttgta ctgtcaggaa gcctttttcc agatcgtgtt ggtaacagac aaacagtagc
     3121 cccatatcca gttgcccgga gttggtgacg cccagtgaat aactgtagcc acgacgcagc
     3181 atcaggctgg actcactctc cgccgtgcgg ggattcgcca gccggatatg gctgtccagc
     3241 gcaatcacct tcccttccgg atcgctggcg taatcgggaa catcatgctc atgctgcatt
     3301 cccagcggcg caccggtttg cttatcacga ccaaaaatcg tctgctgttc tttcagcgga
     3361 gttctgtccc aaaattccac tcgaaactga atcaagcgta ctgcctgata gctgccaccg
     3421 attgtccacg ctggctcctg ctgatctgcc gttacccaca ccactttttg catcaactta
     3481 tcattctggc tatcgggatt ggcagtgccg tctttgaaac ccagcaaatt aatcggcgtc
     3541 tctttgcctt tactacgcgc cgcgtgatcg gaaataaacc cttcccgctt ccagcgcacg
     3601 ctgagcaaat ccggcgtatg tttgatgata tcgcgcagtg catggatcac cgtgtcctgg
     3661 gtgttggcgc aaatttgtag caacacatca ccatgacata acgccgcatc cagcgagtcg
     3721 ttgggaaaac gcgtcatctt ctgcaacttt ttcggcatct gtggcgcaag gccaaagcgc
     3781 tcatcaaaca atgagtgacc caccgataac gtgatggtga gattatcggg cgcaatgtag
     3841 ccgccaagaa tgccggaatc gagtggtggc aggcgcggat ttggcgtttc tggtgccgct
     3901 ccgccctgag tcagaaaagc aaaacgctga gtcaacaagc gaaacaaccg ttcaagatcg
     3961 gctttatcgc tggcaagcac atcaaacgcc accagcatca ttgcggcctg ttgtggcgtc
     4021 aggatccctg cctgatgctc accataaaac ggctgtttct cattgcgtgc atccggtgaa
     4081 agcgtacccg gcgcactttg cgttttttgt gcatgagcga ccggacaact tcccgccagc
     4141 gccagcgcac caatgccttt cagtaaacgt cggcgtgacg gttcattcac gccgttttta
     4201 tcttcatact gcataatgct taatccaatc ccagcacacc gcgaagttgc gccagatctt
     4261 ccgccagcgc agtaatcggt cctttcagtg cattccggtc ggcatcggtc aatttgtcgt
     4321 aggtttcaaa accgtcttta gtacggtatt tcgccagaat ggtatcgacc tttttaaagt
     4381 tggcatcgac ttttgccaac agttccgggt tggctttttg cagttgtgga cgcagcagat
     4441 cgacaatttt ctgcgagcct tcaacgttag cctggaaatc ccacagatcg gtgtggctgt
     4501 agcgatcttc ttcaccgctg attttgctgg ctgccacttc ctcaatcagt ccggctgcac
     4561 cgccgaccac ttttgaaggt gggaaagcca gttcactgat gcgtttttgc aaatcgacca
     4621 catcggtata aagctggtca gcgtactgat ccatgccttt ggtggtgttg tcgccaaaca
     4681 atgctttttc cagacggtgg aaaccggtga attttggatc agcggctttt tgctcgtaat
     4741 catcttcacg ggcgtcaatg ctgccatcca gatcggagaa cagttcagca atcggttcaa
     4801 tgcgctcata gtgctggcgc gtcggcgcat acagtgcttt cgctttttcg atatcgcctg
     4861 ctttaatcgc gtcggtaaag gctttggtgt cggtcaccag ctgcgtggtt tccgccatga
     4921 catacgcttt atatgcagta attgcaccac caagacttaa cagcgcatca ctttgcgccg
     4981 catccgccgt tgcctcacct ttgacgatca acttcccttt cgggttagtc agcagaccgc
     5041 aggtcatatc gtattcgcca ggctgtaaat tcgccgtcat tttctggcta aaaccagggg
     5101 cgatattttc ccgctcttcc accaccatca cgcctttgag gatctcccac tccagcgcct
     5161 tctggctatg gttctgaata atgaactgtg ttttcccggc gttaaccgta atggtcatcg
     5221 gttcgcactg cttatccgtc acggtcactt tgacctgcgt cacatcagcg gcgttagcca
     5281 taaaagcaga agaaaacagc gcagccacgc tcaactgcaa tgcgttacgg cggaagttaa
     5341 tggtcatgac gagactatcc ctttaaagta tgttgtaact aagcaagagt ttgcgtcgta
     5401 tttgttacgc ggagcgagac gctgtcgccc ctgcgcgtgg tggcagagca aatgccacca
     5461 gcgccgggat gagataaata aaccagacgg cgacttcgct gacgctcggc gcttcctgat
     5521 agccaaaaat cccttccatc agcgtgccaa acagcgagtg agttgagagc accgcactca
     5581 tatcgaaggc gatttcctga aagtggttcc acaatccggc ttcatgaaat gcgcgaatgg
     5641 caccagctgc cagccctgcg gcgacgaaga gaataaacag gctggtccat ttaaaaaatg
     5701 caccaagatt gaggcgaata ccgccccagt agagcaagaa gcctagcacc acggcagtag
     5761 caagaccgag cattgcaccc agcggcggcc agatcccgac atcttgttga aatgccgcca
     5821 gcaggaaaaa gaccgactcc agcccttccc ttgcaacggc aaaaaagacc atcatcacca
     5881 gcgcccagcc atgatgattt ccacgctgca atgcgctatc gactgcctgc tccagttgta
     5941 ctttgacgtt gcgcgacact ttgcgcatcc agaaaaccat ccaggtaagg atcaccacgg
     6001 cgatcaccgc cacgatacct tcaaacagtt cctgctcttt ttgcggaaat tcgccggtgg
     6061 tttcgttaat gaagatcccc aagcccaggc acaacgcagc ggcaagcaac acgccaatcc
     6121 acatcacacc aatccatcgg cctcgctggg tacgcttaag atagctggca atcaaactga
     6181 caatcagcgc ggcttcaagt ccttcgcgca acataatgag aaacggaaca aacatgccac
     6241 ccatccttaa acgtttttct gagtcaatct atgcaaagaa aagtaaatcg cagtgatagc
     6301 gattatcatt acggtaaaga aaaatacaag cggaatgttt ataaaatgat gttgaaatgt
     6361 aaacaaagag aggtaatgcc aggggatata aacgattatt ccaggtacaa agatgggatt
     6421 gtttacaacg agccatgtgt tcttgccgga tgccgcgtaa acgccgtatc cagccgacaa
     6481 aaccgtcctg actcaataca tcaaaacttc catgtaaaac tgataagcgg gtgtatcaag
     6541 caatttcgtc catcctcgat tatcaaatct aactttttgc ccggaacata agaacagcgc
     6601 gggtgttggt ggcgtcaaaa tcgctgaaac gttccccgaa ggccggggca gcccacatgg
     6661 atgtgggctg agggcgcgtc ttacaggacg ttacctcgcg cccgacccgg tag
//
LOCUS       DAZKGS010000215         6712 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKGS010000215 DAZKGS010000000
VERSION     DAZKGS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            711..845
                     /locus_tag="ACHOQY_004414"
     CDS             711..845
                     /locus_tag="ACHOQY_004414"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680376.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            1446..2267
                     /locus_tag="ACHOQY_004415"
     CDS             1446..2267
                     /locus_tag="ACHOQY_004415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9680377.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            2267..3373
                     /locus_tag="ACHOQY_004416"
     CDS             2267..3373
                     /locus_tag="ACHOQY_004416"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9680378.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            3467..5188
                     /gene="cptA"
                     /locus_tag="ACHOQY_004417"
     CDS             3467..5188
                     /gene="cptA"
                     /locus_tag="ACHOQY_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9680379.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            5262..6260
                     /gene="lpxM"
                     /locus_tag="ACHOQY_004418"
                     /gene_synonym="msbB"
     CDS             5262..6260
                     /gene="lpxM"
                     /locus_tag="ACHOQY_004418"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9680380.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            6313..6501
                     /locus_tag="ACHOQY_004419"
                     /pseudo
     CDS             6313..6501
                     /locus_tag="ACHOQY_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6564..>6712)
                     /locus_tag="ACHOQY_004420"
     CDS             complement(6564..>6712)
                     /locus_tag="ACHOQY_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9680381.1"
                     /translation="LLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT         1743 a         1447 c         1660 g         1862 t
ORIGIN      
        1 gtctccagga ttcccggggc ggttcatata cccctaattc aactaattgg aggcattacc
       61 atgcattagt gcaagcgcct tgtggattct tttgctaatg gcggaaacat attgtacaat
      121 attgcattta tccctcaata aataaatgag ttatcagatt gcaaaaatct gttatcaaac
      181 taattcaaat aaaaagagat tgatacgacc aacgtacaca aaaattttcg atattcgaac
      241 taaataacag ctcctaatac cgctacactt tagctatcca gtctgcgcct cagaaatgga
      301 cgatgccgtt gaaggactgg tgtatggcaa tgagccgctt tattatcgaa ttcggtgacc
      361 gcttggacgg tcacttcgga gaaaaggcat ttacacagca tcctaaacag gttcagcata
      421 ggtgatatac cgccttttca ttttcttctc cattatttat aagggaggtg tattattatg
      481 caatgttatt ggttttatcc attgtttttt tagtttatga atgttcgcaa aaaaaataaa
      541 tgaacaataa ttttatgtat ttaaaataaa ttaattttac ttctgtaatg gtgtcatgat
      601 tagcagcttt ttaaactaat atcgactttc aataaataag gacaggtaat ggacaacaaa
      661 aatatctgaa cccgggtgag attcataagt tgcacagtgc tgtcttcaaa gtgccacacc
      721 cggaacgtaa tcactgcctg ctccttatgg ggtatcttca tggtgtacaa gcatcagagt
      781 tattaggtat aaaactgtct gatattgatt tgcaggccgg taacctgaat atccggcggc
      841 tctaaacggt ttgtcaactc agcccctcgg ctgaaacagg aggtacagtc cattcgtcgc
      901 tggctgaaac ttcgggatga atgggtagaa gagggatatg aatggttatt taacctctat
      961 ggcaggtctg ctacaggtat tactgttgtt ctggggggca ctggtgtggt taatatctac
     1021 gatgcaagag attctcacga tggcccggtc catcagtgca tgcggcgcga tattgtgcga
     1081 gtatttttca gggggagtgg tatccggatg cagaaaattc cgctactcga ctggttttat
     1141 gcaataaatc aggatgataa tcttctgatt ttttactgag gcggatatct ctcgctgtgt
     1201 ttactgaaat tgcggtattc tgttttttct gaaaaacaga aaatctgatt aaaaagagca
     1261 tatgcgttaa gggaaattta agttctggtt gattaactga taaaaatatt aaaaaatccc
     1321 tgttgtcggt aatctccgga ccattccggg attacacaat aatatcacca tgtgtgatat
     1381 ctatggatcc tcactgttgt ttacgcgagg atattttcag aatgcttaac agacgataat
     1441 tttttatgtt gaatgcaagg catcttcccg tactgatgta tcatcatgtc agtcgttgtc
     1501 cggggcttgt gacactctca cctgaaacat tttgtgaaca gatgaagtgg ctcgcagata
     1561 ataactggcg gacggtaaca tctgctgagc ttgaatattt ttatcagggg ggaacgcttc
     1621 cacgaaagag tgtcatgctc acattcgatg atggttatct tgataactgg ctcagagcat
     1681 ggccggtcct gaaggaatat aaccttcatg cacatatttt tctcattacg gggctgattg
     1741 gtaagggaaa cgtacgtagc aggcaggagc aggaatattc tcaccgcgac tgtgaacggt
     1801 tgattgccga taaccgttca gatgaggtca tgcttcgctg gtctgaagtc cgggaaatgc
     1861 gtgattccgg actggttgaa tttcatgtgc acacccattc acataaacgc tgggacaggt
     1921 tgtctgtatc acgtgctgaa cagtgccggc tcatgaaaga agatattctg gtgggaaaac
     1981 aatgtctgac ggaaaagctg gggttctgta gttcccatct gtgctggcct gaaggatatt
     2041 ataatcggga ttatattaat ctcgccggta agcttggttt ctcttattta tatacaaccg
     2101 aaagaagaat gaactgtcct gaaaatggtt cattacgcat agggcgtatc agcaccaaag
     2161 agcgggaaca ttccggctgg ctgaaacgac gcctttttta ttatacaacg ccactttttt
     2221 cttcagtact ggctcttcat aaggggccaa gattaccaga taattaatga tgaaaatttt
     2281 attcactgaa tcttcgtcag atattggggg ccaggagctg caggcactgg cccagatgac
     2341 tgctcttcaa aagcagggac attccgtttt acttgcctgc agggaaaaaa gtaaaattgc
     2401 tccggaagcc agaaaaagag ggcatgatgt cacttttatt cctttcagga acagccttca
     2461 ccttccttcc atcctcagac ttcgacggat tatcggggaa tttaaacctg atctggtcat
     2521 ctgccatagt ggccatgaca gtaatatagc aggactgagc cgtctgatat gctgtcaccg
     2581 cttcagtatt gtcagacaaa aaacctacat cacccgtaag acccgaacgt tttccctgaa
     2641 ttatctgtgt gatttcattg ttgtgcctag ctcagccatg atggcgcatc tgatggcgga
     2701 aggtgtcagg acaccggtaa cggttattcc accgggtttc gactggcccg cattgcataa
     2761 tgaagccatg cgaccgttac ccctgcatat ccacgcctgg gctgcatcag cagacaatgt
     2821 tccgcttatc gttcaggtgg ggatgctacg tcccgaaaaa ggacatgaat ttatgctgcg
     2881 tgtattgtat cagctgaaga tggaagggaa atcatttcgc tggctcgtgg tgggcgcggg
     2941 gcgtgaagaa tacgaagcac gtctgcgtca gcagacagaa cacctgggaa tgagcggaga
     3001 tgtgttaatg gcaggtgccc ttttcccagc gttaccggtc taccggattg ccagtgtggt
     3061 ggtcatgcct tcggaaaatg aagcgtttgg tatggtactg gctgaagcgt cagtctctgg
     3121 cgtgcccgtt atcgccagtg agacgggcgg gatccctgat gtcattcaga agaatgtgac
     3181 agggacgctg ttacccgtgg gggatgtgtc tgcatggacc ggcgcactgc gcgatttttt
     3241 atcccgaccg gaacgcttcc ggatgatggc agccagcgcc agagaagata ttgagtaccg
     3301 gtttgatatt aacagaacgg cacagatcat tgtgtctctg gctagtcagg ctaaggggaa
     3361 atgtaacaga taacggcgga tcctcttttc agcccgaatt ttgttcagcg gttttccggc
     3421 aagtaacgga gtaatttatc acattgttta aatacgggga cggtcaatgc atttaaatac
     3481 cggacagaac aggcctactt tcagctggag tgcgctggga tgggcaattt tttattttgg
     3541 ctttttttcc actctcctgc aggtcatcat cttcagcagt gggtacagcg gaacgaatgg
     3601 aatacgggac tcactgttat tcagttgtct gtggttgatc ccggtgtttc tctatcctga
     3661 tcggataaaa ataattgcag ctgttgtcgg tttcattctc tggggcacgt cgctggcagc
     3721 actttgttat tattttctct atggtcatga attctctcaa agtgttcttt tcgttatgtt
     3781 tgaaacgaat gccagagagg ctggtgaata tttcagccag tatttcagcc ttaaactgtt
     3841 gcttatctcg ctggtatata ctgcggtgtc cgtttttctg tggacacgtc tgcgtcctgt
     3901 atatattcct ttgccatggc ggagaattgt ctctttcctg ctgctttatg ctctgcttct
     3961 gcatccggtt gttctgaaat cgttaatcag acaggagccg ctgaatgata ctctaggcaa
     4021 actggcatcc cgaatggagc ctgctgcccc ctggcagttt gtatccagtt attatcagta
     4081 ccatcagcaa ctgaatgcac tgacaacctt cctgaatgaa aatagcgcac tgccaccact
     4141 gggtaatctc agggatgaat caggggagag accacgcaca ctggtcctgg tgattggtga
     4201 gtcgacacag cgcgaacgca tgagcttata cgggtatcta cgtgaaacga cgccggagct
     4261 ggatgcactg cgtaaaaccg atccgggtct tactgtgttt aataatgtgg tggcatcgcg
     4321 tccgtacacc attgaagcat tgcaacaggc ccttactttc gccaacgaaa agaatcctga
     4381 tctgtatctg acgcagccgt cgctgatgaa catgatgaag caggcaggct ataaaacatt
     4441 ctggattacc aaccagcaga caatcacagc ccgtaacacc atgctcactg tattttcgcg
     4501 ccagacggac aggcagtact acatgaatca gcagcgaaca caaagtgcgc gtgaatatga
     4561 cactaacgtg ctgaagccgt tccgggaagt gctgaatgac cctgcaccaa agaaactgat
     4621 catcgtgcat ctgctgggta cgcacattaa gtataaatac cgttatccgg aaggtcaggg
     4681 gcggtttgat ggcattacag ggcatattcc cactggatta aatgcgaaag agctggaagt
     4741 gtataacgat tacgataatg ccaatctgtt taacgatcat gtggtggcca gtctgataaa
     4801 ggacttcaga gcaactgcgc cggacggctt tctgctttat ttttcagatc atggtgaaga
     4861 agtatatgat actccgccat ataaaacgca gggacggaat gaagataacc ccacacgtcc
     4921 catgtacact gttccgttcc tgctgtggac ctcggaaaag tggcatgctg cacatccgcg
     4981 agatttttcg cagtatgttg accgcaaata cagtctggct gaactgatcc acacctggtc
     5041 agatttggcg ggactgacat acgatggtta cgatccgacc cgttctctgg tgaatccgca
     5101 attcagggaa actacccgct ggattggaaa tccgtataaa aagaatgggc tcactgattt
     5161 cgacactctt ccgtatggtg agccatagta ttttcttatc ttcggttccg ttctgaaggc
     5221 aggaggatgt gttttcctct gctgtccggt ggactccagg gatggtacca ccttctgctg
     5281 tactctgtta tcataatgag ataagcaggc agataccggt aaatatgaaa aatatcagaa
     5341 ctgagtttat tccccgcttt aaccttacat tatgcttccc ccgttactgg atgacgtgga
     5401 caggtatcgg tattatctgt gtgtttgcaa tggtcccccc cgcactgaga gatccattgt
     5461 tgggaaaatt gggcatgctg gttgggcgtc tggggaaaag cgcacgtcaa cgggccttga
     5521 ttaatttatc gctttgtttt ccggagtaca gtgataaaga aaaggagaat atcgttgatg
     5581 ccatgtttgc aacagcctcc atggccgttg tgcttatggc tgaattagca ctgtccgggc
     5641 ctgataaaat ttcgcatcgt atacgctgga atggtctgga gattgtggag aagatggcac
     5701 agaacaatga aaaggtcatt tttcttgtgc cacatgcctg gggagtggat attcccgcaa
     5761 tgctgatagc tgcgtcgggc agaaaaatgg cagcgatgtt tcataaccag agaaatccgg
     5821 tggtggatta tgtctggaac agtgttcgtc gtcgtttcgg aggaaaactg cacgcacgta
     5881 atgacggtat tgcctccttt gtccgctcag tccggcaggg atactgggga tattatcttc
     5941 ctgatcagga tcatggcccg gaattcagtg agtttgcaga tttttttgcg acgtataaag
     6001 caactttacc ggttatcgga cggctcagca ggatcagtgg tgcacggata ataccgcttt
     6061 tccctgttta tgacggaaag acccatcatc tgactattca tgtcagcccg ccacttgcta
     6121 tacgacagaa gagtgatgct catattgcgc gacagataaa cgaagtggtt gaaaatttcg
     6181 ttcgtccaca tcctgagcag tatacgtgga tcctcaagtt gctgaaaacg cgtaaagaag
     6241 gggaagagga tccttactga ggcaggtttt tcaggataat gagcttaccc agcaatagtg
     6301 gacacacgct aagtgagtaa actctcagtc agaggtggct catatgacaa aaaaacagta
     6361 ccaaccagta aaaaaccccg taaatagcat tcgcctgaat ttcgcagtga agccctgaag
     6421 cttgctgaac gcatcggtgt tgctgtcgca gcccgtgtgc tcagtctgta tgaatcacag
     6481 cttgtaagcg gctcgtcaga actgtattga tatttactga gcggtcgtaa gcgtacagcg
     6541 agggccgtat tgacggggat gtgttattca gctggcagtg ctatgcgcca cggaagcagt
     6601 tcgctgaccc ggttgaccgg ccagtctgct atgacgccag gcacatggcg aaggtagctt
     6661 tctggatcca cgtcattcag tttgcacgtc ccgatcaggc tgtacagtag cg
//
LOCUS       DAZKGS010000216         6675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKGS010000216 DAZKGS010000000
VERSION     DAZKGS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(209..454)
                     /locus_tag="ACHOQY_004421"
     CDS             complement(209..454)
                     /locus_tag="ACHOQY_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680382.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            718..2811
                     /gene="cda"
                     /locus_tag="ACHOQY_004422"
     CDS             718..2811
                     /gene="cda"
                     /locus_tag="ACHOQY_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFP9680383.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2808..3071
                     /locus_tag="ACHOQY_004423"
     CDS             2808..3071
                     /locus_tag="ACHOQY_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFP9680384.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3141..3287
                     /locus_tag="ACHOQY_004424"
     CDS             3141..3287
                     /locus_tag="ACHOQY_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP9680385.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            3994..4182
                     /locus_tag="ACHOQY_004425"
     CDS             3994..4182
                     /locus_tag="ACHOQY_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680386.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTILTDAT"
     gene            complement(4334..4525)
                     /locus_tag="ACHOQY_004426"
     CDS             complement(4334..4525)
                     /locus_tag="ACHOQY_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP9680387.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4593..4916
                     /locus_tag="ACHOQY_004427"
     CDS             4593..4916
                     /locus_tag="ACHOQY_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9680388.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5125..5559
                     /locus_tag="ACHOQY_004428"
                     /pseudo
     CDS             5125..5559
                     /locus_tag="ACHOQY_004428"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5594..6154
                     /locus_tag="ACHOQY_004429"
     CDS             5594..6154
                     /locus_tag="ACHOQY_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFP9680389.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            6161..6394
                     /locus_tag="ACHOQY_004430"
     CDS             6161..6394
                     /locus_tag="ACHOQY_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFP9680390.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVLRLAGQVDRLSRLFITNSR"
BASE COUNT         1661 a         1422 c         1936 g         1656 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg caacttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 taaggggtta tgttaaataa cccttagctt agcggcggcg cagtggttgc caggtgaagg
      241 tgtcatccgg gtcctgtccg gcagtcagtg tggcttctcg atttttgctg gccgttcgtt
      301 catcaagaat ggctttaagt atccgtgccc ggaggcgtcg ctttgtttcc tgcggcattt
      361 tgtccttttt cgtcagcccc atggcacgag cttcagcggc aatggcttct gccagtcggc
      421 gctcttcggc ttcggtcagt ctatggcgtt tcatggtggt ctccgggtgt ttctctggtg
      481 tcataacagt ttaactgttg gctctctcct tatgctaatc agccttcttt catgcctgtt
      541 gccattgagt ttaagaatga ttggttgttt ttcttgagcg ttttcacaaa aacgtaaatc
      601 cagtgggttg acaggattta cgaggtgacg taacttttta ctgtacataa aaccagtggt
      661 tttatgtaca gtttgttgtt tttaacgcat tgttttaaaa gtcaaagagg tgtttttatg
      721 agtgattacg aaggtagtgg tccgacagaa ggtattgatt atgggcactc gatggtcgtg
      781 tggccgtcaa caggactgat ttcgggtggt gatgtgaaac caggaggctc atcaggtatc
      841 gctccatcca tgcctccggg atggggggat tacagcccac agggtatcgc acttgtacaa
      901 agtgttcttt ttccgggaat tattcgccgg attattctgg ataaggaact tgaagaggga
      961 gactggtcgg gatggtctgt cagtgtgcat agcccttggg gaaacgagaa agtttccgct
     1021 gcacgaacag ttcttgagaa tggtttacgt ggtggtttgc cagaaccgtc tcgcccggct
     1081 gctgtttctt ttgcccgtct ggagcctgct tccggaaatg agcaaaaaat tattcgtctt
     1141 atggttacac agcaactgga acaggtaacg gatattccgg ccagccagtt accagcagcg
     1201 ggtaataatg taccggtgaa atatcgtctg atggacctta tgcagaacgg tacgcaatat
     1261 atggctatta tcggaggtat tccgatgaca gtgccggtag ttgatgccgt tccagttccg
     1321 gaccggagtc gtccgggaac aaatattaaa gatgtttaca gtgcccctgt atcaccaaat
     1381 ctaccggacc tggtattaag tgtgggtcag atgaatactc cggttctgtc taatcccgaa
     1441 attcaggaag aaggagttat tgctgagaca ggtaattatg ttgaggctgg ttatacgatg
     1501 tccagtaata atcatgatgt cattgttcgt tttcctgatg gcagtggtgt ttctccgtta
     1561 tatatttcaa ctgtagagat tctggacagt aatggtctga gtcagcgtca ggaagccgaa
     1621 aataaagcaa aggatgattt cagagtcaag aaagaagaag cggtagcccg ggctgaagct
     1681 gaaaaagcaa aagccgagtt atttagtaaa gcaggtgtga accagcctcc tgtatataca
     1741 caggaaatga tggaaagggc taattcagta atgaatgagc agggagctct tgttctgaat
     1801 aacactgcca gttctgtaca actggcgatg accggaactg gtgtctggac tgctgctggt
     1861 gatattgcag gcaacataag caagtttttc agtaatgctc tggagaaagt aaccataccc
     1921 gaagtgagtc ccctgcttat gcggatttct cttggtgctc tgtggtttca ttcagaagaa
     1981 gctggagcag gaagtgatat cgtgccgggg cgaaatctgg aggcgatgtt ttcgctgagt
     2041 gctcagatgt tggctggaca gggcgtggtc attgaacctg gtgcgacgag tgtaaatctg
     2101 cctgttcgtg gacaattgat aaacagtaac gggcaattag ctctggattt actgaaaaca
     2161 gggaatgaaa gtatcccagc tgcggttcct gttcttaatg ctgtccgtga tacagcaaca
     2221 ggactggata aaatcacgtt accagcagta gtaggcgcac cttcccggac gattctggtt
     2281 aatccggtac cacaaccttc ggtgccaaca gatacaggta atcatcaacc cgttccggtt
     2341 acaccagtgc atacaggaac ggaagtaaaa ccggtcgaaa tgccagtaac gacgattact
     2401 cctgtttctg atgttggtgg actacgagat tttatttact ggcgtcctga tgctgctggg
     2461 actggtgttg aagctgttta tgtgatgctt aatgatcctc tggattcagg gcgattttcg
     2521 cgtaaacaac ttgataaaaa atataaacat gctggtgatt ttggtattag tgatacaaaa
     2581 aagaatcgtg aaactcttac taaattcagg gatgctattg aggagcattt atcggataag
     2641 gatacagtag agaaaggaac ataccgaaga gaaaaaggtt caaaagttta ttttaatcct
     2701 aatacgatga atgtggttat aattaagtca aatggtgagt tcttatctgg gtggaaaata
     2761 aatccagatg cggataatgg tcgaatttat ttagagacgg gtgaactatg aacaagatgg
     2821 caatgatcga tttggcgaaa ttatttttag cttcgaaaat tacagcaatt gagttttcag
     2881 agcgaatttg tgttgaacgg agaagattgt atggtgttaa ggatttgtct ccgaatatat
     2941 taaattgtgg ggaagagttg tttatggctg ctgagcgatt tgagcctgat gcagataggg
     3001 ctgattatga aattgatgat aatggactta aggttgaggt ccgatctatc ttggaaaaat
     3061 ttaaattata atcatgagtg attttaatat ctggcattta agtataaatg tcagatattc
     3121 actcatgaca aggagatgtc atgaaaaaaa agataggggg atctatgatt ctcgttcttg
     3181 ctgttttgtg tctgactgct tgccaagcta actatgtaag ggatgttcag ggtggaacaa
     3241 ttgctccatc atcatcttcg aaactaatcg gggtggcggt tcagtgaaaa atattaaagg
     3301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaacccgc
     3361 cttgtggcgg gtccttccgt ccgatcagaa gtcttcgacc tcttctaatc ggcggtggta
     3421 actggcttgg aggagcacag tcaccaaaac tgtcttttca gtttagcctt aactggttca
     3481 taacttcaag agcaactcct ctaaatcagt taccaatggc tgctgccagt ggcgttaagt
     3541 cgtgtctttt ccgggttggc cccaagagaa cagttaccgg agaaggtgca gcagtcgggc
     3601 tgaacggggg gttcctgcat acagcccagc ccggagcgaa ctgcctaagt cgccccgaga
     3661 taccagaaga cgtgagctat gagaaagcgc cacgtgtccc gcaagggaga aaggcggaaa
     3721 aggtatccgg taatcgatat ggcgggaacg tgggggcgca ggagggagcg attcaccgga
     3781 aacggtgatg atctttaagc cacgtcgggt ttcgccaccg ctggcggatt tcatggttga
     3841 gcctcacggc tcccacagat gcaccgaaaa agtgtctgtt ttgtgaagtc cgtcaggggg
     3901 gcggagccta tggaaaaagc gcctccggcg cggccttttt cggggttttc gtttcttgct
     3961 cacatgttct ttccggcgtt atcccctgat tctgtggata acactatcac cgcttttgag
     4021 tgggcagata ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa
     4081 gcggaaaagc gcctggacga gcattttctc cttacgcatc tgtgcggcat ttcacacccg
     4141 gcatggtgca cttttcatac catcctcact gatgccacat gattaagcca gtatacactc
     4201 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgctaaaa cccgctgacg
     4261 cgccctgacg ggcttgtctg ctcccggcat ccgctcacag gcaagctgtg accgtctccg
     4321 ggagctgcat gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg cagctgcggt
     4381 aaagctcgtc agcgtggtcg tgaagcgctt cgcagatgtc agcctggtcg tcggcgtcca
     4441 gttcattcag tttttccagc agggtcagcg tctggcttct gatgaagcgg gccatgttaa
     4501 gggcggtttt ttcctgttta gtcacagttc tgtttctcca tgtgtgggga gtctgtcgga
     4561 ggtttttaat gataccggtg aaacgcgaaa ggatgctcac gatacgggtc acggatgagg
     4621 aacatgcgcg gctgctggca cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg
     4681 tctgcctggg ggcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt
     4741 tgcgccagtt cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagtg
     4801 gacagtggtc ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg
     4861 aactgagtga gctgagggat gaggtccgta aacagggaga acgcgatgat agttaagttt
     4921 catgccaggg gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt
     4981 aaccgggaag gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat
     5041 gccacgccgt ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagacactg
     5101 ccaccgggag agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt
     5161 gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg
     5221 aattttgtca tcccgaatat ggagctggcg agcggaaaac ggctccagcc ctattacgac
     5281 cgggcggacc gtccgcgaat caatgcctgg cagacactgg tgaatcacca ttacgggctg
     5341 cacgacccga acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa
     5401 gcgaaacagg aggccgcaga agcgattacg aggggactgg aggctcttta ccatgccgga
     5461 gcgctaaaaa cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggtggtg
     5521 aggacaacca ggagcagtat cagtatcgcc gacccggagg gcgggcgtaa tctcaggctg
     5581 aaaggggcgc tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa
     5641 agagcgtgcc gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc
     5701 tgtagacagg gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac
     5761 agcagcggtg aaagaacaac tggagacgct gaacagtgcc gtgaacgcgc agttcagcgc
     5821 cacagagaac gaacttgccc ggcggcagga agagctgctc tggaagctgg tcaaagggcg
     5881 gatactgtac ccgtcgctga cggcgctttg cgtcacggtc gggatttttc tggcgtcatg
     5941 ggggctgatt cagtggcagg aaagccgtat tgcggcgaac atcctggcta tcagggacca
     6001 ggaagagacg ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag
     6061 gaacgggaaa tttctggtgt tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga
     6121 gaagaagaac gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca
     6181 cttactgaac gccttagagc aattgcagca ggactatacg cagcggctga aagactggga
     6241 gagcgccttc acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact
     6301 gagggaacag tgcgacgact tgagtcagca ggtgctgaga ttagccgggc aggtggaccg
     6361 cttgagtcgg ttatttatca cgaacagcag ataagggatg aacaggaaag gcaggcacgc
     6421 ctggaacggg agcgtgcgcg gtcctggcat gaagagcagg aacgggaagc acgggtatgg
     6481 cgtggcccgt caatggggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg
     6541 gaacgcgtta aaaacgcatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt
     6601 gcctctttgc cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat
     6661 caactgaaaa gatgg
//
LOCUS       DAZKGS010000217         6606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKGS010000217 DAZKGS010000000
VERSION     DAZKGS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..384
                     /locus_tag="ACHOQY_004431"
     CDS             <1..384
                     /locus_tag="ACHOQY_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9680391.1"
                     /translation="LGIYLYTDVALPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKK
                     AGNTKLGEFGNYSNDWQTLELVFTASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTL
                     TDITKNATYGVEIESLVLEINSPAA"
     gene            408..875
                     /locus_tag="ACHOQY_004432"
     CDS             408..875
                     /locus_tag="ACHOQY_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680392.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            868..1503
                     /locus_tag="ACHOQY_004433"
     CDS             868..1503
                     /locus_tag="ACHOQY_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP9680393.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            1515..2081
                     /locus_tag="ACHOQY_004434"
     CDS             1515..2081
                     /locus_tag="ACHOQY_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP9680394.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            2078..2428
                     /locus_tag="ACHOQY_004435"
     CDS             2078..2428
                     /locus_tag="ACHOQY_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP9680395.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            2432..3328
                     /locus_tag="ACHOQY_004436"
     CDS             2432..3328
                     /locus_tag="ACHOQY_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP9680396.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            3321..3851
                     /locus_tag="ACHOQY_004437"
     CDS             3321..3851
                     /locus_tag="ACHOQY_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP9680397.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATHDGEVITIYPDD"
     gene            3854..4567
                     /locus_tag="ACHOQY_004438"
                     /pseudo
     CDS             3854..4567
                     /locus_tag="ACHOQY_004438"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            5132..5986
                     /locus_tag="ACHOQY_004439"
     CDS             5132..5986
                     /locus_tag="ACHOQY_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680398.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTPDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPGSGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGNHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNEENTVKNIA
                     FNYIVRLA"
     gene            5986..6564
                     /locus_tag="ACHOQY_004440"
     CDS             5986..6564
                     /locus_tag="ACHOQY_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9680399.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
BASE COUNT         1681 a         1703 c         1861 g         1361 t
ORIGIN      
        1 ctgggaattt atctgtatac cgatgtagcg ttacctgacg tcgtggcgat gaccgggact
       61 ggtaacccgt tcctgatgtc gttcttcacc cagaccacag acggcaaact gaatctgatg
      121 catcacaaga aagcaggaaa cacaaagttg ggcgagttcg ggaattacag taacgactgg
      181 cagacgctgg agctggtgtt caccgccagc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggtcataaaa gacagtctga cactggggct gaatgcactg
      301 acgctgacgg atattaccaa aaatgcaacg tatggcgttg agatagaaag tctggtgctg
      361 gaaataaata gtcctgcagc gtaaggatga acaggagagc aaaacagatg cttaagacaa
      421 acagcctccg gaagggcatg attcatgggt gtcgctggtg tcaggccaac ccggaaaaat
      481 tcaccatttt cgtggagagc ggcaacattg aaacgaccgg agaaacgccc tcgtttgttt
      541 accgctatca gatggtgatg tttgtcatgg attacgccgg ggagctggac gacctcacgc
      601 tgccgctact ggcgtggtta tccgaaaatc agccgcaatt gttgcttaat ccggagcgta
      661 atcaggacat caaattctcc gccgttatca atgacgatga cagcgccgat ctcctgttta
      721 cgctccctct gcgggaacgc gttcgcatca cgcgcagcag tcagggggca ccgcaggcag
      781 aacacctgca ggagccaaaa ccccgtctgc catcttccga aggcgactgg tcgcatgtat
      841 tccaggatgt gacgtggggt gaaagcgatg gataaggcat tcacccgcgt ggatgaaacc
      901 tttgaggcta tccgcgacag cctgaatcag caggccatca ataacatcgc cagaaagctg
      961 gcacaggatt tacgccgcgc ccagcaggcg cgtatccggt cacagaaagc gccggacggg
     1021 accgcgtgga caccacgcag acgccgcgta acccggatac aggagcgcat tcgctttatc
     1081 tggaataacg aagcacgcac gctgaaaaac tggcatcacg acacggggaa atacgggcga
     1141 accattaccg ggtgggatga ggataaaaac aatatccgca cgttttaccg ggatgacatc
     1201 gaccgctttc tggaaatacg cacccggcgc atcaaccagg acagcacaaa gcgcgtcccc
     1261 atgttcgtaa aactgcgcac cgcccgctac ctgaaagccc gtgcagatgc ttccggtgtg
     1321 acggtgggtt acagcggcgt ggccgcacgt attgcccgcg ttcatcagtt cggtgagcgc
     1381 gatcaggttg cgccgggcat tttcaccgat tacccggtac gtgagctgtt gggcatcagt
     1441 caggcagatg aacgcctgat ttataacacg gtgctgggcc ggattgcgga ggctgtacgg
     1501 tgagcgcaga actcatgcga ctgctgagca acatcatccg cactgggatc atctctgaag
     1561 ttgatgagaa gtcctggtgc gtgcgcgttc gcagcggcga actggaaaca ggctggttgc
     1621 gctggaacac cacgcgcgcg ggagccttca atgtgtggct gccgccatca cccggcgaac
     1681 aggtggtaat tgcctgcatt ggcggcaacc cggaaaccgc catgataatt ggcagcctgt
     1741 ggagtgatgc caatccggcc cccggcaaaa gcctgaaaga aatcgtggtc agcgcgccgg
     1801 atggcgcggt gttccgctac gacgcggacg caggcgcact gagcgccagc ggcatgaaaa
     1861 cggccaccct gcaggcatcc gtcagtgtga cactggacac gcccgtcgtg gaatgcacag
     1921 accttctgag aacggcgacg cttgacgtca caaaaggggg aaagatgagc ggcaatatca
     1981 cgcacagcgg cggcaatttc acctcaaacg gcatcacagt gcatacgcat aaacacggtg
     2041 gcgttaaagg tggcagcgat tcgacaggag gcccgcagtg acaacccgct acacaggaat
     2101 gaacccggac ggaacgggaa acctgaacga tatggagcat ctgaaacagt cagtcaggga
     2161 catcctgacc accccgctgg caagccgggt tatgcgacgg gaatatggca gccttgtgcc
     2221 cgatttaatt gacgaaccca tgaataacac cactcgtctg caatgcatga gtgctgccgt
     2281 gattgcgctg acacgatggg aaccccgcat tgccctggac gccatcgacg ttgtctggaa
     2341 ggcaggaggc cgcgccgggg tgacgctgtc gggcactgtc atgcagacca tgcagaatgt
     2401 tgaattaacc atcacgctga gggagtaaat catgcctgcc gttgaccttt cccagttacc
     2461 ggaacccgcc atcatcgcgg agcctgactt tgaggcaatt ctggctgaca caaaggccat
     2521 gatgattgcg tcctatcctg ccgaacagcg tgaagccgtc tccgccgcgc tggagctgga
     2581 atcggaaccc ctgaacgtta tcgcccagac aacagcgttt cgtgaaatgc tgttacgcca
     2641 gcgggtcaat gagggggcac gcgcctgcat gttaagccac agcgccggga cagacctgga
     2701 caacctcgcg ggcaatatga acacaaagcg cctgaccatc actccggcaa cggataccac
     2761 cgacgcagtg atggaaagtg acacctcgct gagactgcgg gcgcagcggg cgtacgatgg
     2821 cctgagtgtt gctggcccgt caggtgcata cgagtatttt gcccgcagcg ccagcggtct
     2881 ggtgcgtgat gcgcgggcta tcagtccgtc tccggcaaat gtgacggttt ccatcctgtc
     2941 cactgaaggc gacggcacag caacggaggc gttgcttaat accgttcgcg ccgttctgaa
     3001 tgcagaggat acccgcccgg tggccgaccg cctgacggta cagagcgcca gaatcgtgac
     3061 atggcggctg aatgcaaaac tgtactttta ccccggcccg gaatccgaac ctattctggc
     3121 ggcggctgaa tcgtcgttca ggaagtggct ggctgagcag gggcttatcg gtcaggacgt
     3181 ggcgttgtcc gccattgctg ccgcactgca tgtgcacggt gtgcaacgcg tggagataat
     3241 cgaacccaca cagaatatgg ccatcagcga catacaggcg gcgcgctgtg agtcgttcac
     3301 catcagcgaa ggtgggcgca atgagtaatt cactgttacc accatcagcc agcagtttca
     3361 tgcgttgtgc cgaagctgtc ggaacgcgca ttacagacat cccggtagac ctcaacacgc
     3421 tgtggtcgcc ggacacctgc ccggtgcacc tgctgcctta tctcgcctgg gcattttccg
     3481 ttgaccgctg ggatcgcaac tggccggaag agacaaaacg acaggtgatt cgtgatgcat
     3541 ggctgataca ccgacacaaa gggaccatca gcgcactgcg cagggccatt gagccgctgg
     3601 gatacctcat tcgcgtgtct gagtggtggg agttcggcgg agaaccggga acatttaccg
     3661 ttgaagtcgg cacactggac agtggcgtga cggaggaaat gtatctggaa atggagcggt
     3721 tgattgctga tgcccgtccg gtcagccgcc acatgacagg gctgaatatc attcaggaaa
     3781 ttccggggga tatttttgca gcggcggcaa ctcatgacgg tgaagttatt accatttatc
     3841 cggacgatta agcatgagta ccacaacacg aaaatttaaa accgttatca ccgatacagg
     3901 tgccaaaaaa ttagctcagg cagccgcgcc agatggtaag cctgtccgcc tgactcatat
     3961 ggccgtgggc gacggtggcg gcgcgttgcc cacaccagac agtaagcaga cccgtctggt
     4021 gcatgaggtg tggcgacaca ctgttaatcg cgtcatcctg gacgcaacac atcagaaccg
     4081 cattattgcg gagctggtta ttcctccaga aacgggcgga ttctggatcc gggaaattgg
     4141 tgtgtttgat gagcacggcg atttaatcgc ggtgggcaat actgccgaaa gttacaagcc
     4201 agccgttgcc gaagggtccg gacgtgcaca aacatttcgc accattctga ccgtatccag
     4261 cactgccact gtggcgctta ccgtggataa caccatggtg atggccacag tggattacgt
     4321 ggataacgaa ctgaaagagc atgaacagtc acgatgtcac ccggatgcct cgctgaccgc
     4381 aaaaggcttt gttcaactca gtagcgccac taacagcgat tctgaaacgc tggctgcaac
     4441 gccgaaagcg gttaaggtcg cgtatgatct tgctaacgga aaatatacag cgcaggatgc
     4501 cacaacagcg cgaaaaggcc ttgtccagct tagtagtgca accaacagta catctgaaac
     4561 gctggcggca acatcaaatg cagtaaaagc tgcctatgac aatgctgaaa aacgtctgca
     4621 gaaagctaag aatggtgagg atatctctga taaagacacc tttacgaaaa atatcggtgc
     4681 ctgccgtgca tatagtgcag agctgaatat tggtggagat agtgaagcat ggacaactgc
     4741 gcagttgatt ttttggctag agagtcaggg ggcatttaac catccttact ggatgtgcaa
     4801 aggctcatgg gcttatgcaa ataataaggt cattacagat acaggttgcg gaagtatttg
     4861 tcttgcaggt gctgttgtgg aagttattgg cacccgcggc gcaatgacca tacgcattac
     4921 cacaccgagt acatccagcg gtgaaggcat ccctaatgct caatttactt atattaatca
     4981 tggtgatgct tatgctcctg gctggcgaag ggactataac tccaggaata agccaacagc
     5041 atcagagatc ggggcgttac cgtcagatgg gacagcagta tcgtcagtta atctggcttc
     5101 aaaaggtcgg ctgaccgccc tgacagataa tatgcagggg gccacaggtc tggagttata
     5161 cgaggcgtat aacaacggat atccaacaac gtatggaaat atcattcacc tgaaagggat
     5221 gacagccgtt ggcgaaggcg aattactcat cggctggagt ggtataagcg gtgctcatgc
     5281 tccggcattt attcgttcac gacgggatac gcccgacgca aactggtcgc cgtgggcgca
     5341 gctttacacc tcggctcatc ctcctgaaga gttttatcca gtcggtgcac cgattccgtg
     5401 gccatcagat accgttccgt ctggttatgc cctgatgcag gggcagactt ttgacaaatc
     5461 tgcatacccg aaacttgcag tcgcttatcc gtcaggcgtt attcctgata tgcgtggctg
     5521 gacgattaag ggcaagcccg gcagtggtcg tgccgtattg tctcaggaac aggacggcat
     5581 taaatcgcac acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc
     5641 gtcgtttgat tacggtacta aaacgaccag ttcatttgat tacggcacaa aaactacgaa
     5701 tagcgctgga aatcattcac acaatatacc tgttggtcac actggcgcgg ggaatggtgt
     5761 atcagccggt tataacgctg cgttaggtac tggtaccacg tcgagcgcag gcgagcatgc
     5821 tcacaatgta tatatcggtg cccataacca cactatcggc attggtgctc atgcccattc
     5881 tgtcattatt ggtccccacg gacacaccat caccgttaac gctacgggta acgaagaaaa
     5941 caccgtaaaa aacatcgcat ttaactatat tgtgaggctt gcataatgac attcagaatg
     6001 agtgaacact cacggaccat aaaaatttat aatctactga ccggaaccaa tgagtttatt
     6061 ggtgaaggtg atgcatacat tccacctcat acaggtctgc ctgcaaacag taccgatatt
     6121 gccccgccag atattccggc tggcttcgtg gccgttttca acagtgatga ggcatcgtgg
     6181 catctcgttg aagaccatcg gggtaaaacg gtttatgacg tggcatcagg ggactcgtta
     6241 tttatttctg aactcggtcc attaccggaa aatgttacct ggttgtcgcc gtatggagag
     6301 tatcagaagt ggaacggcac atcctgggtg aaagatgcag aagcagaaaa actgtttcgg
     6361 ataagggagg cggaagaaac aaaaaacagc ctgatgcagg tagccagcga gcatattgcg
     6421 ccacttcagg atgccgtaga tttggatatt gcaacggagg aagaggcatc gttactggct
     6481 gcatggaaga cataccgggt attgttgaat cgtgttaata cagcggtagc agcggatgtt
     6541 gagtggccag tcgccccaca ataaagagaa aaagccatcg attgaaatat agatggcttt
     6601 atgtac
//
LOCUS       DAZKGS010000218         6568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKGS010000218 DAZKGS010000000
VERSION     DAZKGS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..622)
                     /locus_tag="ACHOQY_004441"
     CDS             complement(<1..622)
                     /locus_tag="ACHOQY_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9680400.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     KAMYPERKLFAIGWVKHKCKPGTKWPEIQAELRNWKKRQDAERKETG"
     gene            complement(716..907)
                     /locus_tag="ACHOQY_004442"
     CDS             complement(716..907)
                     /locus_tag="ACHOQY_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9680401.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(904..1092)
                     /gene="dicB"
                     /locus_tag="ACHOQY_004443"
     CDS             complement(904..1092)
                     /gene="dicB"
                     /locus_tag="ACHOQY_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9680402.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            1660..1869
                     /locus_tag="ACHOQY_004444"
     CDS             1660..1869
                     /locus_tag="ACHOQY_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680403.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(1870..2508)
                     /locus_tag="ACHOQY_004445"
     CDS             complement(1870..2508)
                     /locus_tag="ACHOQY_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680404.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESMANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2520..2672)
                     /locus_tag="ACHOQY_004446"
     CDS             complement(2520..2672)
                     /locus_tag="ACHOQY_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9680405.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            complement(2949..3236)
                     /locus_tag="ACHOQY_004447"
     CDS             complement(2949..3236)
                     /locus_tag="ACHOQY_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9680406.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(3236..3427)
                     /locus_tag="ACHOQY_004448"
     CDS             complement(3236..3427)
                     /locus_tag="ACHOQY_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP9680407.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(3455..3856)
                     /locus_tag="ACHOQY_004449"
     CDS             complement(3455..3856)
                     /locus_tag="ACHOQY_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680408.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            3965..4237
                     /locus_tag="ACHOQY_004450"
     CDS             3965..4237
                     /locus_tag="ACHOQY_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9680409.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            4221..4646
                     /locus_tag="ACHOQY_004451"
     CDS             4221..4646
                     /locus_tag="ACHOQY_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9680410.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4853..5308)
                     /locus_tag="ACHOQY_004452"
     CDS             complement(4853..5308)
                     /locus_tag="ACHOQY_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680411.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            5387..6502
                     /locus_tag="ACHOQY_004453"
     CDS             5387..6502
                     /locus_tag="ACHOQY_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9680412.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
BASE COUNT         1708 a         1459 c         1619 g         1782 t
ORIGIN      
        1 ttccagtctc tttgcgctct gcgtcctgcc gttttttcca gttacgtaat tcagcctgaa
       61 tttcgggcca tttggtaccc ggcttacatt tgtgtttaac ccatccgata gcgaacagtt
      121 tgcgttccgg atacatagct ttaatttcag gcgttttcat cagtgcttca acgatatgcc
      181 cgtcaaaggt agccacgtct tcttgcagta attcctgcgc gtcaatcgcc atatcaacgg
      241 tgatgttttc acatgtaccg aacttaacca ggaccgcgtt ctgtacttca agggacagct
      301 tgtcaaaatt gacgttaata ggatcggatt ctggttcgac cggaataaag gaagcggatt
      361 cctcatccca gcggttttcc tgcatatatt cggtatccca ggagtcgata gcagggcggg
      421 gcatgccggg tttatcttcg cagacaagaa atttataagc gcagtcctga gcagcaggat
      481 attgctccag gaattgccag gtaaatttgg cacgggcgcg gcgttcatca ccggcttcaa
      541 tggcagtggc tacagcaact gcgccctctt cttttattgc ctgttcgtcc ggaatggcgg
      601 cgcaaataaa gactttactc attttgtttt aacctcatta cagatttaag ggtgaacaaa
      661 tccctgccat tgctggcata taaaaatgaa accggatatt aattacggtg ctgttttaaa
      721 gtcctgccgg tatttcgtta ttattagtgt gagtagtttt atctaccgga taacagttac
      781 cgggaatttt ttgttctgct gctgcagcca tgcattcttt cattgaaccg tataagccag
      841 tcaccagctc aagagattcg ccggaaacaa gataaactgt cagaacgagt gcaaatgttg
      901 tattcattgt ttatatcctt tttgcagcag gtccagacga gccagcattg aaggaatgca
      961 tacttcattt aacaggtcct gctcgagttt tctctgctta atggcgtctt caataaatgt
     1021 tttgtctcca gtgataacgc caatttcgaa acgaagttca gacgtgctgg cattacatga
     1081 taacttttcc attatcgcgt cctcaacaat gaattttgtg atgcggtgcc tggtgcctcc
     1141 aggtgacgtt aaccagttaa caattaacgc cggatacaga gaatccaccc ataacactgt
     1201 ttttggtttt aactgttccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt
     1261 ttaaaaaagg gcggcagagc agtcacggag taaaactgat accgccaaat gtcaccagaa
     1321 tattgataac agagggcgtt gtagcggggg tgtcacttaa gcgtatggtc aacctgacaa
     1381 cccggtgtcc tcaactgggg aaggaataac cccgccatac ttaccgccgc gccatttcgc
     1441 ggattgccac aaccggaagc gcacgttcga agaaatctaa cgacaagcct tctaagggaa
     1501 agagcttcgc cgtacgcttt cgcgttatgc cctgactttt cagggaaata tcctttcagt
     1561 aaactgtcag taccggattc ttatccgtgt ccggcgcacg accacacgtg acagcgtgtt
     1621 ggtctccatt tttaacccag aacctcaatg gaggataaaa tgccaaacaa aaaaagaaat
     1681 ccgcttattg aaaaacagat tgaatgcctg gtaaatcaac tcaggcaatc agggttatta
     1741 aaaactcatt cagagttgag gctcacagaa tcagcattcg acgataaatt aaataatgtc
     1801 ctttataatg gcattattga ttttaatcgt tctgttggtc gccgcggccc tgctggtgtt
     1861 tccttataat taccagtcaa tccagagtgg accgtgttca gcgtaaatat aactgtacac
     1921 atccagatta tatttgtggt ctgttaagaa caggccgcaa atacatgccg aagcttccag
     1981 tgcagcggct ctgttactga ataaccatgt agcaacattc cagcgttttt ctgcatccca
     2041 gtctttctca aggcctgata ccatgaagaa accgttagtg ttgccatcaa ataattctgt
     2101 ttccaaattt ttaagcaatg cctgatggac tcttgccagg tattccgccg gaatttcgcc
     2161 acgaattcta atgagattgt cataaacaaa catgttcccc gcatatggcg atttttcttt
     2221 cttgtttttt aaaccagcat catgagcaaa ctgatcaatt tcttcttccg ttggtttcgt
     2281 attgatgttt tgcgctgtcg tttctgcaat tttatttgcc atactctctg agtcgtgttt
     2341 atttatagac gcacagaaat acaatccggt aaacgcatcg cgcacattac gagccatatt
     2401 atcagtgtct tttttcgtta ccgattccaa ttcaagttcg ttcagacgat gacgaagtgt
     2461 gtgtgctgca atctcctgga ttgaaggagg taaatcttta aattccatcg tcaacctcat
     2521 cagtcagtgt ttctggctaa ccagcgacgc gcgccagctt cagttttaaa cgttttgctt
     2581 ttggtatacg tcatcgcggt gaacgtaccg tcctggttgg gaaacacgcc gcataccaga
     2641 gattcgttgt tgccaagatc gatagtatcc atgttgacct catttcccct taacgccggg
     2701 tagcggaaca aaaacctgct gcatagttat taaagttgaa ccctgccgtc atgttcatac
     2761 gcctcgggct ggctacttac cccctgacca ctgcttggta actcgaagta ttgcccggcg
     2821 ttctgtgggg cggggtgggt ggttggtgta tgtaatctac aattaaaaac tgttttgtgt
     2881 caacagtttt taattgtttt tttgggcaaa aaaatccctc gaaagaggga gtatgaaaat
     2941 tgttcaactc agatagagaa gggaaattgt cgccgagaat gtgtcacgct tacaatctca
     3001 atgcttgaag cagctactct gtacaggatt atgtagttag ggtgggtcac gatctctctc
     3061 aatccagaaa cccgttcgct tggtggatat aagtacggat gctcagatag aggtaatacc
     3121 gatgtttcaa tgcgtatttt tagtctacgt gctgccggtg ggttctcctt ggcgatgtaa
     3181 gttatgatct ggcgcaaatc atcgcgagca gacggtagcc ataaaatggg taacattact
     3241 cgctcttgtt agttacagca atttgagcaa taagattttc catttcagcc attacctcgt
     3301 catgtggaat tgcggggcga gtgtctgcaa ggcttgacgt tactttagtg cgcaaccatt
     3361 cgttgtaact gttttcttgt tcggtagttt cgaattctga aactatcgga gaaagggctg
     3421 tacccatggc ataactcctc ttcttgtact gtggtcacgc ccggcggctt ttttgtgccg
     3481 ccagccacct agcaatggtt tcttccattg attttttctt gtctttgatt tcttgaagca
     3541 ttttttcttg gtcttcctca ggaaacgcac taaaagcttg gagcagttcg cgttggcgag
     3601 gaccaatttt catcgtgtca ggggtgaaaa tttgctcaca ctcttcagga ggcaataaaa
     3661 accaatgcaa tggatgccct gaaacctcaa ccagtttatc caaacttgag gctttaggtg
     3721 tagccttacc gctgacccat tgttgaacag tttgttgtgt cacaccaatt ctacgggcaa
     3781 gctcagcctg gctccatcca gtttcctgaa gaagcttgct gattctgtac atagatactt
     3841 ctagggcgct catcattatt caattttaca ggtaaatact gttaaaagca tcacaataaa
     3901 aaactgttga ttgcatacag ttttttattg taggctttgc ttatagtttt tagaggaggg
     3961 caaaatgcta gatagcactc gcgaaaaaat taggcagaaa tacactcagg ctgaaatagg
     4021 tcgttatatg ggggtcgctc aacagactgt ttggcaatgg tttagctttg gcgttccccc
     4081 aaagcaggta attccgttat gccaactaat gaagtgggaa gttaccccgc atgaaatccg
     4141 cccagatatt tatcctaacc caaccgacgg tttacctgtt ggatgtaagg ttaacacatc
     4201 aaatgcgccg gagttgattc atgaaaatca agcatgaaca catccgcatg gcgatgaatg
     4261 cctgggcgca tccggacggc gaaaaagtac cggctgcgaa aattaccaaa gcgtatttcg
     4321 agctgggaat gacgttcccg gaactgtatg acgacagcca tccggaagcc atggctcgca
     4381 atactcagaa aattttccgc tgggtggaga aagacactcc tgatgcggtt aaaaaaattc
     4441 aggcgttgtt accagcgatc gaaaaagcga tgccgcctcc gctggtggcc cgaatgcgca
     4501 gccacagttc cgcttatttt cgggagttgg tagagacgaa ggaacggctg gtgaaagata
     4561 ttgatgattt cgttgcatca gcgatcgttc tgttcgatca gatgaatcgt ggtggcccgg
     4621 caggaaacac tctggctgtg cattaattgg gtaataaata tgagtaatga caaaaaattg
     4681 acactgagcg tttacgaaaa cagtccgcac atctggcgtg gcggtttatc tgatgtggag
     4741 ctggcagagt ggttgataca taaagctaat gcgctgctct ggcgtttgtc agccagagaa
     4801 cagcgcaagg aaaccagaat aaagctggct gatgcagaag cgtgtgccgg gcttattgag
     4861 gattatacaa atcttggtat ttcttcagca gagagtgatc ccattcagcc tctgagcagg
     4921 gagtcaatcc agcacgctgg ttgtatggca catcttgtaa ctgctcgtca acatgaggtg
     4981 ggtattggat cacttccggt gggatattcg ctgattccag agctggttga agcaagaaaa
     5041 tcagttcaga aaaagagaga tgacgcactt caattattga gagagcacta tggcgcgata
     5101 ccagaatgcg aacagcatcg ataccctgaa ggttatgaat ggatgcagtc tctttttgaa
     5161 gttcgctaat caatatgtcg agacgaaggt atgtttcggc gcgcagccag gctctgtaat
     5221 ccgggagcat ttcggggctg ttacaccagc ggtttgttgc tgcaacattt aatacatgag
     5281 cctgataaag gcttttcaaa aaatacatgt cgaacctcct ctggttctgt cgattgggaa
     5341 ccacagatta tatccggagg aaggttcggc accagatgag gtagccatgc gtgattacgc
     5401 aaaagtttct ccgcgattct ggctgggaga aacggggaga gaacttagaa aggcgggtgc
     5461 agaagcgcaa gttgttgctt tttacctgat gacatcccct cacgcaaata tgctgggttt
     5521 gtattacctg ccagttttat accttgctca tgaaaccggg cttggtctgg aaggggcttc
     5581 aaaggggctt aaaagggctg ttgaagctgg tttttgtagc tatgaccatg atgcagagat
     5641 ggtctgggtc catgaaatgg cagcctggca ggttggggaa acgttgaagc ctggcgataa
     5701 ccgttgtgca ggtgtcagga atgagtatgc atcattacct gaaaacgctt ttctgtcagt
     5761 gttttacgac agatataaaa cggatttcca tctggatgtg aggcggaata atagccgaaa
     5821 ttcggtaagg ggcttcgaag gggcttttaa ggggcttcga agccaagaac aggaacagga
     5881 gcaggagaaa gaacaggaac aggacaaaaa cactatggtt catggcaaaa aaaacaccac
     5941 gaaccaggca ggggatgttc agaccgtcaa tcctggtcag ccagcaggca cgacaccgga
     6001 agccgattca gcgtatgcgc tgaaagccga ttcgggcgct gtgcagcagg tgatgaccgc
     6061 agggtcggag caatcacacc aactgcagca gcctgaagcc gattccgcca ttcagcggga
     6121 agccgatcgg gtagtcccgg aaagcaccgg gcagtctgtg ggacgagtgg attatccgga
     6181 tgtgttcgaa caggtctggc gggaataccc gttgcgtgct ggggcaaacc cgaagaaatc
     6241 cgctttcagt gcctggaagg ccagattgcg cgagggggtg ccaccagaga ccatgctgga
     6301 tggtgtgagg cgttacgcga gatacctggc ggcgaccggg aaagcgggaa cggaatttgt
     6361 tcagcgagcg acgacgtttt ttgggccgga ccggaatttt gaaaacccct ggttgctccc
     6421 ggtaagcggc acgaacaacc agcgttgtgt gaatcatatt tctgaaccgg ataccgaaat
     6481 tccgccggga ttcagggggt gatgtggcat gaaaaacatt gcggcagccg gggttcttga
     6541 acgtattcgc agacttgcac cacaggcg
//
LOCUS       DAZKGS010000219         6516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKGS010000219 DAZKGS010000000
VERSION     DAZKGS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            112..840
                     /gene="gadW"
                     /locus_tag="ACHOQY_004454"
     CDS             112..840
                     /gene="gadW"
                     /locus_tag="ACHOQY_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9680413.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1203..4316)
                     /gene="mdtF"
                     /locus_tag="ACHOQY_004455"
     CDS             complement(1203..4316)
                     /gene="mdtF"
                     /locus_tag="ACHOQY_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9680414.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4341..5450)
                     /gene="mdtE"
                     /locus_tag="ACHOQY_004456"
     CDS             complement(4341..5450)
                     /gene="mdtE"
                     /locus_tag="ACHOQY_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9680415.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5558..5836
                     /locus_tag="ACHOQY_004457"
     CDS             5558..5836
                     /locus_tag="ACHOQY_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680416.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5837..6364)
                     /gene="gadE"
                     /locus_tag="ACHOQY_004458"
     CDS             complement(5837..6364)
                     /gene="gadE"
                     /locus_tag="ACHOQY_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9680417.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1631 a         1588 c         1593 g         1704 t
ORIGIN      
        1 aaattttggt cctaccaaat ctggcagttt ttgcgctaag aaacagtctg gcaacatttc
       61 attagtatac tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat
      121 gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcataaaata
      181 tcgctgcata acgagagtat cgtgctgctg gagaaaaatt tggcagacga ttttgcgttt
      241 tgttcaccgg atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa
      301 aatattcgtc agctaccacg caatttaggg ttacatagca aagaccgtct attaattaac
      361 cagtcacccc ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta
      421 aattcaccga tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag
      481 aaagaactga tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc
      541 cttattagct ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat
      601 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
      661 ttactcgctt ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg
      721 catactattg cagaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga
      781 caatattatg gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga
      841 catattttgc atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc
      901 tgatgatttc tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt
      961 attttcagga ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt
     1021 acgggaatgt gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc
     1081 tattttcagt gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt
     1141 gaatggttag caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca
     1201 cgttacgctt ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg
     1261 aagtaaattg ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg
     1321 ttttgcgcgc cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag
     1381 gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct
     1441 atcggcgttt tcccttcttt ctgcatcatc tcaacggcaa attcgacaat caggatggcg
     1501 ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt
     1561 aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc
     1621 accgagaacg gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc
     1681 agtgaaatgg catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt
     1741 ccggtccatg agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc
     1801 atagcgtcac cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg
     1861 ccgttgtagc gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa
     1921 agcggtgcca tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac
     1981 atacggaacg gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag
     2041 tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc
     2101 acacccattg cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc
     2161 aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc
     2221 tcgtttcgtg cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata
     2281 tcaaaacctg acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg
     2341 gctttattga tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt
     2401 tcctcaccga cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc
     2461 ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct
     2521 tttgtaagat aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca
     2581 ccggaaggta actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag
     2641 gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg
     2701 tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt
     2761 tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgcg
     2821 gctttcagaa tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc
     2881 agcatggagg agatcagcgt gatggagaac tggcggtaga tctccccggt tgcgccgctc
     2941 ataaaggcca tcggcataaa cactgcggaa agaacaacag caataccgac cagcgcacgt
     3001 tggatctgcc ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg
     3061 acacgctcga cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc
     3121 accatcccga acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac
     3181 gtcccgagaa taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc
     3241 aaaaacagat acatgaccag gaagacgagg atgatagcct caaccagtgt tttaaaaact
     3301 tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt
     3361 gcggggaaat aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg
     3421 gcgtttgctc cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc
     3481 gccacggtgg aataatcttc cgctccaagt tcgacgcggg cgacatcacg cagcagcact
     3541 tgcgaaccat cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga
     3601 cgcgtctgca caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc
     3661 agttgaccac cggaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt
     3721 accagattgt atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa
     3781 ccaaagagct gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac
     3841 gctacatagt ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct
     3901 accatcagaa tattactgct cgacttatca acgctaatcc cctgctgctg caccgcttca
     3961 ggtaatgaag gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga
     4021 gatgtcccag tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa
     4081 gacatgtaca tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag
     4141 tcttctaccg tttgcgcatc ggcacctgga taggtagcgc tgacggtaat ggtcggtggc
     4201 gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc
     4261 ataataatgg caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa
     4321 gtccccttaa gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag
     4381 gaaattgctc gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga
     4441 tcgccagcct gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca
     4501 atttcgcgta gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg
     4561 gcgttgtggg tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc
     4621 actaatgccg tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc
     4681 cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg
     4741 gtctggctgt agcgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc
     4801 tgtttgattt gcccactggc gacctcttct ttcatgcgta agaaatcttg caccgactgc
     4861 gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta
     4921 gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag
     4981 gtgacattcg cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg
     5041 acattggctt ctgcttcatt caactgggtg cgcgcggtat cgtaatcctg acggctaacg
     5101 tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta
     5161 gagagcgctt tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga
     5221 tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta
     5281 atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg
     5341 ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc
     5401 gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag
     5461 aataacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga
     5521 gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg
     5581 catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt
     5641 caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa
     5701 gggttctact ggtggataca cataccaggg gaataaagtc gttttagcct ctcctttagt
     5761 aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca
     5821 gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac
     5881 gtcttagttt cagcttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa
     5941 tattgagcgt cgacgtgata ttgctttcat ttttattttt cagaatcagt tgcataattt
     6001 tgaactgatt atgtgaaaaa cataattgtg aattcttatg gggcaagtgt ttaccataag
     6061 taatctcaag aagatttttg atactttctt tgcggctaac aaaataaata ggcgctttag
     6121 cttttagttt actgatgtga taaggtgcca aaacaataat tctctcggca tctaatttct
     6181 ccagaaattt aatcgcttct tcatcaagga tatgattgtg ataggtatca atgataacct
     6241 tgaagggttt attgcctact tttttgatct ctgacaggga attgattttt atcatttcgt
     6301 gattatcttt caactgccaa aagccctgta aaagaaaaga atctttcgtc atgagaaaaa
     6361 tcataacttg ctccttagcc gttatcgttt cgaacatgtc atccttttca ttatttacat
     6421 ccttgtccga atcgttgttc aatatagtaa acgcctataa gaactttgcc cttcgtgaac
     6481 taatacgtct attaacacta atcaatactt cagatt
//
LOCUS       DAZKGS010000220         6472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKGS010000220 DAZKGS010000000
VERSION     DAZKGS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..352)
                     /gene="yaaX"
                     /locus_tag="ACHOQY_004459"
     CDS             complement(56..352)
                     /gene="yaaX"
                     /locus_tag="ACHOQY_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9680418.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(566..1852)
                     /gene="thrC"
                     /locus_tag="ACHOQY_004460"
     CDS             complement(566..1852)
                     /gene="thrC"
                     /locus_tag="ACHOQY_004460"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9680419.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1853..2785)
                     /gene="thrB"
                     /locus_tag="ACHOQY_004461"
     CDS             complement(1853..2785)
                     /gene="thrB"
                     /locus_tag="ACHOQY_004461"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9680420.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2787..5249)
                     /gene="thrA"
                     /locus_tag="ACHOQY_004462"
     CDS             complement(2787..5249)
                     /gene="thrA"
                     /locus_tag="ACHOQY_004462"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9680421.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5276..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5331..5396)
                     /gene="thrL"
                     /locus_tag="ACHOQY_004463"
     CDS             complement(5331..5396)
                     /gene="thrL"
                     /locus_tag="ACHOQY_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9680422.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(5610..6296)
                     /gene="yjtD"
                     /locus_tag="ACHOQY_004464"
     CDS             complement(5610..6296)
                     /gene="yjtD"
                     /locus_tag="ACHOQY_004464"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9680423.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         1548 a         1792 c         1571 g         1561 t
ORIGIN      
        1 cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta cccggcattt gtcatttagc
       61 ggtgatgttt gcctggacca tgaccgccgt gatgatcatg atgagctttc ttatggtgac
      121 gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc tcgccattca taatgttgtt
      181 tccaccagcc gtggtcgcgc cagtgaccgc catcccagta ataaccgcga ttatcacgat
      241 cgcctatctg taattttact gacgggacta acgtaatttc cgcagcctgc gctgccatgg
      301 gagcgaccag aaccagggaa agtgcgagta cgatagattg catctttttc acaggttact
      361 ccttttatca ttgcccgtta tgggtcgata tccggagatt acgcgatgag aacggcggtt
      421 gttattctcc gcaatcctaa tctctaagtt accgcattta ttgagaattt ctcctttttc
      481 cctgtcgttt ctctccatat tttcttcaca aaaaagccgg gctgcataaa agcaaacccg
      541 gcctgattga gataatgaat agatgttact gatgattcat catcaattta cgcaacgcag
      601 caaaatcggc gggcagatta tgtgaaagca agggtaaatc agcacgttct gccagctctt
      661 ttggcagatc caacgtttca ccgagaatcg cttccacgct ctctttaaat ttcgccggat
      721 gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa ctggtcacgc agcgcacgat
      781 acgctacggc agcgtgcggc tccgaggtgt agcccagttc ttttaactca cgcattgtct
      841 gttgcgtggt ttcatcatcc acggctgcat aacccagctc tttcagttgc cagattttgc
      901 ggcggaacaa ctcttccaca cgcggccagt tgttcggctg actcacgtcc atcgcgttgg
      961 ataacgtcgc ctgagtcgct ttgggggacc actgaccgtc gtgcaggaaa cgtggcacgg
     1021 tatcgttcac gttggtcgca gcaataaaac gtttcaccgg cagaccgagt gacttcgcca
     1081 gcagacccgc cgtcaaatcg ccgaagtttc cgcttggtac cgaaacaacc agctggttgc
     1141 gcgcctcctg cggcagctgc gcaacagctt caaagtagta gcaaatctgc gccagcaaac
     1201 ggctgatgtt aatcgagtta gctgagttta accccagcgc cactttcagt tcttcatcat
     1261 caaacgcctg cttcaccagc gcctgacagg catcgaaatc gccgtcgatg gcaacagttt
     1321 cgatattgcc gcccaatgta cagaacagtt tttcttgcag tggactgatt ttgcctcgtg
     1381 gatagaggat aaccactttc acattcggca aaccgtagaa agcatgagcc actgccgctc
     1441 cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt atcacccgca atatgggtca
     1501 gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc cagcgttggc ccgtggaaca
     1561 attccagaca accgacatcg ctttcaacat tggcgaccgg agccgggaag gcaaacgccg
     1621 cgcgcacgcg ctcttccagg atttcctgcg ggatttcatc accaataaac gccgagagga
     1681 tcttcgcact gcgggtgaca aaatccagct tcagcatctc atcaatttca gtcaggctga
     1741 attccggcag gtcatgcgga aaaaacagcc cctgattttt gcccaacccc tgggttacgg
     1801 cttgcgcaaa gctgacctgc tcgttgtgat ctttcagatt gtagagtttc atttagtttt
     1861 ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg aacaaaacct tcctgatttt
     1921 gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc ggtatccggc ttgtcacaca
     1981 gagcgaacaa ggtcgggccg gagccggaga taccgctcgc taccgcgccg atttccgcga
     2041 ccgcctgccg cgcctgccgg aagcctggca gtaatcgttc acggtagggt tcagcgataa
     2101 catctttcat cagcttcgcg gcaagctcag gctgacggga atagcaggcg tgaatgaagc
     2161 ctgccagatg tcgcccgtgc gcaatgcaat cctggcggcg atactgcgcc ggtaaaatag
     2221 cccgggcttc tgccgtcgag actttaatcc ccggatacgc cagcacccac agccactcat
     2281 caaacccagg cacttgctgg ctgatgatgt cgttttcttc gatcatcaac tgcataccac
     2341 ctagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc ggagatacgt ccttccagct
     2401 cgcccatcaa agccagcaaa cgagtgtcat taagcggctt gccgcagtgt tcattcatcg
     2461 ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa gcccgaaccg atcggcatat
     2521 tcttttccag agtcatcgcc actggaattt gcttgccaag ctcctggcaa aaacgctccc
     2581 agcactgata aacgatattt tcccgtggtt ctgacggcag cttatcggca aagcgcccga
     2641 ggttgttgag actgaatatc tctgccgcct caaccgtaac tacatctccg agcaatgcac
     2701 catcaacggg tgtcaccgcc gccccgagca catcaaaccc gacgctcata ttggcactgg
     2761 aagccggggc ataaacttta accatgtcag actcctaact tccatgagag ggtacgcagc
     2821 agatcggcaa agacaccagc agcggtaacg tcattgcccg caccatatcc gcgcagcacc
     2881 aacggcagcg gctgataata gtggctatag aaggccaggg cgttttcgcc atttttcact
     2941 ttgaacagcg gatcattacc atccacttcg gcaatcttca cgcgacagac gccatcttca
     3001 tcaatattgc caacatagcg caaaactttt ccttcatcac gggccttcgc cacgcgcgcg
     3061 gcaaagcgat cgtcgagctg tgacagattc gccataaaag cggcaacatc accctcggcg
     3121 ttaaactctg cgggcagcac aggttcaatt tcaatatccg ccagctccag ttcacgtccc
     3181 gtttcacggg cgagaatcaa tagtttacgc gccacatcca taccagaaag atcatctcgc
     3241 ggatccggtt cggtataacc catttcccgt gccagcgtgg tcgcctcgga gaaactcatg
     3301 ccttcgtcta acttgccgaa gatataagaa agcgatcctg aaagaatgcc ggagaacttc
     3361 atcaattcat caccagcatt gagcagattt tgcaggtttt caataaccgg taatccagcc
     3421 ccaacgttgg tgtcatagag gaatttccgc cgcgattttt ccgccgcatg acgcaacaga
     3481 tggtagtaat ccatcgacga ggtgttggcc tttttgttcg gcgtgacaac gtggaaacct
     3541 tcgcgcagga agtcggcata ttgatccgcc actgcctggc tggaggtgca gtcaacaatg
     3601 accgggttca gcagatgata ttctttcacg aggcgaatta agcgcccgag attaaacggc
     3661 tctttggctt gcgccagttc ttcctgccag ttttccagat ttaggccatg cacattggtg
     3721 agcagtgcct tcgagttggc aacaccgcag acacgtaagt cgatatgttt attcttcagc
     3781 cagctttgtt gacgcttcag ttgctccagc agcgcaccgc caacgccacc gacgccaatc
     3841 acaaacactt cgataacctg atcggtattg aacagcatct gatgagtaac gcgcacgcca
     3901 gtggtcgcat catcgttatt taccacgaca gagattgagc gttcagaaga tccctgagca
     3961 atagcgacaa tgttgatatt ggcgcgggcc agcgcggcaa agaatttcgc cgagatccca
     4021 cgcaaggtac gcataccatc acctaccacc gagataatgg ccagccgttc cgtcaccgcc
     4081 agcggctcca gtaagccttc tttcagttcc aggtagaact cttcctgcat tgcccgttca
     4141 gcccgcacac agtcgctttg cggaacgcag aaactgatac tgtattcgga agatgattgc
     4201 gtaatcagca ccacggaaat acgggcgcgt gacatcgcgg caaagacgcg cgccgccatg
     4261 ccgaccattc ctttcatccc cgggccggaa acgctgaaca ttgccatgtt attcagattg
     4321 gaaatgccct tgaccggtaa ttcgtcttca tcacgactgg caccaatgag cgtacctggt
     4381 gcttgaggat ttccggtatt tttaatcagg caagggatct ggaactgggc gatgggggta
     4441 atggtgcggg ggtgaagaac tttagcgccg aagtaggaaa gctccatcgc ttcctggtag
     4501 gacatcgact tcaacaacct cgcatcgggc acctgacgcg ggtcgcaggt atagaccccg
     4561 tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac aggcagccag caccgccgcg
     4621 gagtagtcgg aaccgttgcg tccaagtacc accagttcgc ctttttcatt accggcggtg
     4681 aagcctgcca tcagcaccat gtgatcagcc ggaatacgac ttgccgcaat acggcgggtg
     4741 gactcagcaa tatcgacggt agattcgagg taatgcccca ctgccagtag tttttcgacc
     4801 ggatcgataa cggtaacgtt gtgaccacgc gcttctaaca cgccggccat aatggcgatc
     4861 gacattttct cgccacggca aatcagcgca gcgttaatgc tatccgggca ctgccctaac
     4921 aaactaatgc catgcaggac atgttttatt tgggcaaatt cctggtcgac gaaagttttc
     4981 aattgcgcca gcgggaatcc cggctgggcg gcggcgagtc ccgtcaaaag ttcggcaaaa
     5041 atacgttcgg catcgctgat attgggtaaa gcatcctggc cgctaatggt tttttcaatc
     5101 atcgccacca ggtggttggt gattttggcg ggggcagaga ggacggtggc cacctgcccc
     5161 tgcctggcat tgctttccag aatatcggca acccgcagaa aacgttctgc atttgccact
     5221 gatgtaccgc cgaacttcaa cactcgcatg gttgttacct cgttaccttt ggtcgaaaaa
     5281 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     5341 ccgttacctg tggtaatggt gatggtggtg ataatggtgg tgctaatgcg tttcatggat
     5401 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     5461 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     5521 tgtcagagac tcttttttta atccacacag agacatattg cccattgcag tcagaatgaa
     5581 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     5641 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgctgtaac
     5701 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt
     5761 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgt cggttgttgt
     5821 attaatgttg ctaattgata gcaatagacc atcactgcct gccccagatt gagcgaagga
     5881 taatccgcca ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc
     5941 aacccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
     6001 tctaacagcg gcaccagttc aactggcgtg gcgtagtaat gatatttcgc ccgactgcgc
     6061 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt caaccagtgt cgggaaaact
     6121 ttaatattat caataatatc accagatcca tgtgcgaccc agcgagtggc tggctccaga
     6181 tgtgcctgac tatcgacaat ccgcagttcg ctaaacccca tcgttttcat tgcccgcgcc
     6241 gctgccccaa tattttctgc tctggcgggt gcgaccagaa taatcgttat acgcatattg
     6301 ccactcttct tgaccaaatg accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
     6361 cgaatttaca aatccttaac gtaagtcgca gaaaaagccc tttacttagc ttaaaaaagg
     6421 ctaaactatt tcctgactgt actaacggtt gaattgttaa aaaatgctac at
//
LOCUS       DAZKGS010000221         6429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKGS010000221 DAZKGS010000000
VERSION     DAZKGS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1267
                     /gene="tssI"
                     /locus_tag="ACHOQY_004465"
     CDS             <1..1267
                     /gene="tssI"
                     /locus_tag="ACHOQY_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP9680424.1"
                     /translation="QFRYSAQIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYE
                     VYDYPGRFKSAHGQNFARWQMDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNR
                     EWQVVASELHGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVV
                     TGPAGEEIFCDEHGRVRVRFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQE
                     VIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDAT
                     GREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITG
                     VLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPD
                     GIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            1295..1756
                     /locus_tag="ACHOQY_004466"
     CDS             1295..1756
                     /locus_tag="ACHOQY_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9680425.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1776..6429
                     /locus_tag="ACHOQY_004467"
                     /pseudo
     CDS             1776..6429
                     /locus_tag="ACHOQY_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
BASE COUNT         1555 a         1753 c         2000 g         1121 t
ORIGIN      
        1 ccagttccgc tacagcgcac aaatccgccc ttcttccgtg gtgaccaaag actacacctt
       61 taaacgcccc ggctgggccg gacgttttga acaggaaggc cagcaccagg actaccagcg
      121 cacgcagtat gaagtgtatg actaccccgg acgtttcaag agcgcccacg ggcagaactt
      181 tgcccgctgg cagatggacg gctggcgaaa caacgcagaa accgcgcggg gaatgagccg
      241 ctcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc aggcgaacct
      301 aaaccgggaa tggcaggtgg tggcaagtga actgcacggc gaacagccac aggcggtgcc
      361 aggacggcag ggagcgggga cggcgctgga gaaccatttt gcggtgatcc cggcagacag
      421 aacatggcga ccacagccgt tgctgaaacc gctggtcgac ggcccgcaga gcgctgtcgt
      481 gacaggaccg gcaggcgagg aaatcttctg cgacgaacat ggtcgcgtgc gggtgaggtt
      541 caactgggac cgttataacc cggcagacca agacagttcg tgctggatcc gtgtggcaca
      601 ggcgtgggca ggcaccggtt ttggccacct ggcgataccg cgtgtgggtc aggaggtgat
      661 tgtagacttc ctcaacggcg atccggacca gccgatcatt atggggcgca cctaccacca
      721 ggaaaaccgc acccccggca gcctgccggg aacaaagacg cagatgacca tccgctccaa
      781 aacgtacatg ggcagcggat ttaatgagct gaagtttgat gatgcgacag ggagagaaca
      841 ggtctacatc cacgcgcaga agaacatgga taccgaagtg ctcaacgacc gtaccaccac
      901 cgtaaaacac gatcaccgcg aaaccgtaaa aaatgaccag acggtcacga tccaggaagg
      961 taaccgcctt cttacggtgg aaaaaggcca caagatcacc ggagtactga aagggtcttt
     1021 atctgaggat gtctttcagg acagaggcac gattgccggt tcggtgcatg ttgacgctgt
     1081 aaacaatggt ggcgaaggcg acggtataca ggcttatacg gcgattaagg aaattttgct
     1141 ggctgtggag gaaagcaaaa ttgcgctgac gccggatggc attcagctac aggtcgggga
     1201 atcgacggta atcaggctgt cgaaggatgg catcaccatc gtgggcggtt ctgttttcat
     1261 caactgattc actcagatta tcagggagta ttttatgcag ttcactttta atgaaggcca
     1321 cattcaactg ccgtctcaat ggcaggatca gtcaatgcag gtcctggtgt ccacagataa
     1381 cagtggcatc aacctggtca ttacccgcga agccgttcca cagggaaccc tgacaccaga
     1441 gctgtaccag gaaacgctgg cgctgtacca gggcaaactt gatgggtaca ccgaacatgc
     1501 ctgccgggaa atcactctgg cagaggcccc ggcctggctg ctggattatt cctggaacgg
     1561 tccggaagat gaggggaatc aggggcggat tagccagata gccgtattcc agcggcgtgg
     1621 agatacgctg ctgaccttca ctttcagcac ctccttatcc ctgaagaaca gtcaaaagac
     1681 gatgctgctg gaggtgataa agagcttcac cccgttgcca ccggagaatg acatccaaaa
     1741 ggaccagccg cgctgacata aacgagggat aacacatgag tgaaggacca ggcgggccac
     1801 agggagcgac cgcaggcggt acgctggcaa tgcgaatgct gtcacagcag gcgatggtcg
     1861 ccagccagat gaaacgggca gccaacgaca aagccattgc acagatgctg gcagcaaaga
     1921 agtccggccc acctgccgcc aggctgggcg atgaaattca gcataaaagt tttctggggg
     1981 cactggcagg ggccgtgctg ggggcgatag tgaccatcgc agaaggttgc ctgattatgg
     2041 ccgcctgcgc caccggccct tatgcgctgg ttctggtgcc tgcgctgatg tatgccagct
     2101 ataaggcgag tgattatgtg gaggagaaac agaaccagct tgaatcatgg ataaacagct
     2161 tttgtgacac ggacggcgcc atcaataccg gttctgaaaa tgtaaacatt aacggaaagc
     2221 ccgctgcaag ggccgccgtc acccttcccc ctcctccccc acctggagca atacctgaaa
     2281 tcccacaggg ggaaccctca tggggtgata ttgccactga cctgcttgaa tcggcagcgg
     2341 aaaaagcagt accactggcg aaggcctggg ggaacgctgt tatcaccctg acggaaagca
     2401 atgccggttt tatggatcgc gtcagcgccg gcgcatcgct tctgtttccc gccggtccgg
     2461 tattaatgga gtttgccacc atggtgggcg ggcgtagcga aatcaaaaaa gatgtggatt
     2521 tcccggaagc cggtgaggac acggcgctct gcgacaagga gaacaaacca ccgaggatag
     2581 cccagggcag tagcaacgtc tttatcaaca atcagcctgc cgcgcgcaag ggcgacaaac
     2641 tggagtgcag tgcggcgatc gtgggaggtt cgccggacgt ctttattggc ggtgaacagg
     2701 tgacctatct ggatatccag ccggagttcc cgccgtggca gagaatgatc ctggggggaa
     2761 taacgatagc cagctatctt ctgccgcctg cgggactgct gggaaaactg aagaatctgg
     2821 cgagactcgg caaactggga aacctgctgg ggaaaagcgg gaagctgctg ggcgcaaagc
     2881 tcggcgcgtt gctggggaaa acaggtaagt cgttaaaaag tattgccaat aaagtcatca
     2941 gatgggtaac agatcctgtc gatccggtaa ccggcgcata ctgcgacgaa cgtaccgact
     3001 tcaccctggg ccagaccctc cccctctcct tcacccgttt ccacagttct gtactgccgc
     3061 tgcatggcct gaccggcgtg ggctggagcg actcctggag cgaatacgcc tgggtgcgtg
     3121 aacagggaaa tcgggtagat attatcagcc agggagccac gctgaacttc gccttcgacg
     3181 gtgacagtga tacggcggtt aacccgtatc acgcccagta cattctgcgc cgccgcgacg
     3241 attacctgga gctgttcgac agggatgcac tgagcagccg cttcttttat gacgcctttc
     3301 cgggaatgcg tctgcgccac ccggtgactg acgataccag cgatgaccgc ctggcacaca
     3361 gccccgacga ccggatgtat atgctgggcg ggatgagcga caccgccagc aaccgcatca
     3421 cgtttgagcg cgacagccag taccggatca cgggtgtcag tcacaccgac gggatccggc
     3481 ttaaactgac gtaccacgcc agcggctacc tgaaagccat tcaccgcacg gataacggca
     3541 tacagacgct ggcgacctac gaacaggatg cgcgggggcg gctgacagaa gcggatgcgc
     3601 ggctggacta ccacctgttt tatgagtacg acgctgcgga ccggatcatc cgctggtccg
     3661 ataacgacca gacgtggagc cgtttcacct acgatgcaca gggccggtgc gtgaatgtca
     3721 ccggggcgga gggctattac aacgccacgc tggactatgg tgacggctgc accaccgtga
     3781 cggacggcaa gggcactcac cgttattact atgatcctga cggcaatatt ctgcgggaag
     3841 aagcgccgga cggcagtacc accacgtatg aatgggatga attccatcac ctgctggccc
     3901 gccactcccc tgccgggcgg gtggagaagt ttgaatacaa cgccgcactc ggtcagttaa
     3961 gccgttacac ggcggcagac ggcgcggagt ggctgtaccg ctatgatgag cgcggcctgc
     4021 cggtaagcct ggtgtcgcca cagggcgaag agacccggct ggcatacacc gctcaggggc
     4081 tgctgtcggg gatattccgc caggatgaac ggcgtctggg catagagtac gaccaccaca
     4141 accggccgga aacactcacc gacgtgatgg gccgtgaaca ccacaccgaa tacagcggtc
     4201 acgacctgcc ggtgaagatg cgcggccccg gcggtcagtc agtgcggtta cagtggcagc
     4261 agcaccataa actgagcggc cttgagcggg cagaaaccgg cgcagaagga ttccgctatg
     4321 accgccacgg caacctgctg gcgtacacgg acggtaacgg cgttgtctgg acaatggaat
     4381 acggcccgtt cgatttgccg gtggcgcgaa cggacggtga aggccaccgc tggcagtacc
     4441 gctacgataa agacacgctg cagctcacag aagtcattaa cccgcagggc gagtcatacc
     4501 gttatattct ggacaactgt ggccgggtga cggaagagcg tgactggggc ggcgtggtct
     4561 ggcgttaccg ctatgacgct gatggcctgt gtaccgccag ggtcaacggc ctggaggaaa
     4621 ccatcctcta cagccgggac gccgcaggcc gcctggcaga agtcatcacg ccggaaggca
     4681 aaacgcagta tgcgtatgac aaatccggca ggctgacggg tatcttcagc ccggacggca
     4741 catcacagcg caccggctat gacgaacgcg ggcgggtgaa tgtcaccact cagggccgac
     4801 gggccattga ataccactac cccgatgaac acaccgttat ccgctgtatc ctgccaccgg
     4861 aagatgaacg cgacagacac cccgatgaat ccctgctgaa aaccacgtac cgctacaacg
     4921 ccgccggaga actgacagag gtcattctgc cgggggatga gacgctgacg ttcagccgtg
     4981 atgaggcggg acgtgaagtg ctccggcaca gtaaccgggg ttttgcctgt gaacagggct
     5041 ggaatgcagc cggccagcct gtcagtcagc gcgccggatt ttttccggag gaaaccacat
     5101 ggggcgggct gctcccctca ctggtacggg agtaccgtta cgacagcgcg ggcaatgtgt
     5161 cggctgtcac cagccgggaa gattacggac gggaaacccg gcgggagtac cggctggacc
     5221 ggaacggcca ggtcacggcg gtgacagcct caggcaccgg gctgggctat ggcgaaggcg
     5281 atgagtccta tggctatgac agttgtggct acctgaaggc gcagtctgcg ggcaggcacc
     5341 ggataagtga agagactgac cagtatgccg gaggccaccg gctgaaacag gccggaaaca
     5401 cgcagtatga ctatgacgcc gcaggccgga tggtcagccg caccagacac cgtgacggct
     5461 accgcccgga aacagagcgg ttccggtggg acagccggga ccagctgacc gggtattgca
     5521 gcgcacaggg tgagcagtga gaataccgcc acgacgccag cggcagacga acagagaaac
     5581 gctgcgaccg gaagaaaatc cgcttcacgt acctgtggga cggcgacagt attgcggaaa
     5641 tccgggaata ccgcgatgat aaactgtaca gcgtacggca cctggtgttt aacggctttg
     5701 agctgataag ccagcagttc agccgggtac ggcaggcgca tccgtcggtg gccccgcagt
     5761 gggtgacgcg gacgaatcat gcggtgagcg acctgacggg ccgcccgctg atgctcttta
     5821 acagtgaagg taaaaccgtc tggcggccgg gacagaccag cctgtggggg ctggcactca
     5881 gcctgcccgc agacaccggc tacccggacc cgcgcgggga gtgggacccg gaagccgacc
     5941 ccggcctgct gtatgcggga cagtggcagg atgcggaatc ggggctgtgc tataaccggt
     6001 tccggtacta cgagccggaa accggaatgt acctggtgag tgatccgctg gggttgcagg
     6061 ggggtgagca gacttaccgg tatgtgccta atccactggg gtatattgat ccgctggggc
     6121 tggcaaaaac ttctgttccc gcagaaaaaa taagcctttc ggataaagca agagatcttt
     6181 ttagacaagg aaaagtaaga gaagcattag atgttcatta tgaggatctt gtacgaagaa
     6241 agcttggtgg tatatctcaa gaaattgccg ggcgtgaata tgatgttgtt actgacaaga
     6301 tcatagcgca ggtcaagagg acttatagct ctatagataa tccaaaaaac ttcttaagta
     6361 agtctactcg gacacaaatt aagaagacaa ttgagctggc tgaagaacag gggaaagagg
     6421 ctcagtttt
//
LOCUS       DAZKGS010000222         6345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKGS010000222 DAZKGS010000000
VERSION     DAZKGS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..965
                     /gene="iaaA"
                     /locus_tag="ACHOQY_004468"
     CDS             <1..965
                     /gene="iaaA"
                     /locus_tag="ACHOQY_004468"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9680426.1"
                     /translation="GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEA
                     GESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHL
                     RNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEGA
                     TVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCY
                     ANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGL
                     IAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            952..2823
                     /gene="gsiA"
                     /locus_tag="ACHOQY_004469"
     CDS             952..2823
                     /gene="gsiA"
                     /locus_tag="ACHOQY_004469"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9680427.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2843..4381
                     /gene="gsiB"
                     /locus_tag="ACHOQY_004470"
     CDS             2843..4381
                     /gene="gsiB"
                     /locus_tag="ACHOQY_004470"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9680428.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4399..5319
                     /gene="gsiC"
                     /locus_tag="ACHOQY_004471"
     CDS             4399..5319
                     /gene="gsiC"
                     /locus_tag="ACHOQY_004471"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9680429.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5322..6233
                     /gene="gsiD"
                     /locus_tag="ACHOQY_004472"
     CDS             5322..6233
                     /gene="gsiD"
                     /locus_tag="ACHOQY_004472"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9680430.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1394 a         1525 c         1868 g         1558 t
ORIGIN      
        1 tgggcaaagc agtcattgca attcatggtg gcgcaggtgc aattagccgc gcgcagatga
       61 gtctgcaaca ggaattacgc tacattgagg cgttgtctgc cattgttgaa accgggcaga
      121 aaatgttgga agcgggcgaa agtgcgctgg atgtggtgac ggaagcggtg cgtctgctgg
      181 aagagtgtcc actgtttaac gccggaattg gcgctgtctt tacgcgtgat gaaacccatg
      241 aactggacgc ctgtgtgatg gatggtaaca ccctgaaagc cggtgcggtg gcgggcgtta
      301 gtcatctgcg taatccggtt cttgccgccc ggctggtgat ggagcaaagc ccgcatgtga
      361 tgatgattgg cgaaggggca gaaaattttg cgtttgctcg tggcatggag cgcgtctcgc
      421 cggagatttt ctccacgcct ttgcgttatg aacaactact ggcagcgcgc aaggaagggg
      481 caaccgtcct cgaccatagc ggtgcgccac tggatgaaaa acagaaaatg ggcaccgtgg
      541 gggccgtggc gttggattta gacggcaatt tggcggcagc cacgtccaca ggcggaatga
      601 ccaataaatt acccggacga gttggcgata gtcccttagt gggtgccgga tgctacgcca
      661 ataacgccag tgtggcggtt tcttgtaccg gcacgggcga agtcttcatc cgcgcgctgg
      721 cggcatatga catcgccgcg ttaatggatt acggcggatt aagtctcgcg gaagcctgcg
      781 agcgggtagt aatggaaaaa ctccctgcgc ttggcggtag cggtggctta atcgctatcg
      841 accatgaagg gaatgtcgcg ctaccgttta acaccgaagg aatgtatcgc gcctggggct
      901 acgcaggcga tacgccaacc accggtatct accgtgaaaa aggggacacc gttgccacac
      961 agtgatgaac ttgatgccgg taatgtgctg gcggttgaaa atctgaatat tgcctttatg
     1021 caggaccagc agaaaatagc tgcggtccgc aatctctctt ttagtctgca acgcggtgag
     1081 acgctggcaa ttgttggcga atccggctcc ggtaagtcag tgactgcgtt ggcattgatg
     1141 cgcctgttgg aacaggcggg cggtttagta cagtgcgata aaatgctgtt gcagcggcgc
     1201 agtcgcgaag tgattgaact tagcgagcag agcgctgcac aaatgcgcca tgttcgcggt
     1261 gcggatatgg cgatgatatt tcaggagccg atgacatcgc tgaacccggt atttactgtg
     1321 ggtgaacaga ttgccgaatc aattcgtctg catcagaacg ccagtcgtga agaagcgatg
     1381 gtcgaggcga agcggatgct ggatcaggta cgcattcctg aggcacaaac cattctttca
     1441 cgttatccgc atcaactctc tggcgggatg cgccagcgag tgatgattgc gatggcgctg
     1501 tcatgccgcc cggcggtgct gattgccgat gagccaacca ccgcgctgga tgtcactatt
     1561 caggcgcaga tcctgcaatt aatcaaagta ttgcaaaaag agatgtcgat gggcgttatc
     1621 tttatcactc acgatatggg cgtggtggca gagattgccg atcgggtact ggtgatgtat
     1681 cagggcgagg cggtggaaac gggtaccgtc gaacagattt ttcatgcacc gcaacatcct
     1741 tacacccgtg cgctgttagc tgctgttccg caacttggtg cgatgaaagg gttagattat
     1801 ccccgacgtt tcccgttgat atcgcttgaa catccagcga aacaggcccc ccccatcgag
     1861 cagaaaacgg tggtggatgg cgaacctgtt ttacgagtgc gtaatcttgt cacccgtttc
     1921 cctttgcgca gcggtttgtt gaatcgcgta acgcgggaag tgcatgccgt tgagaaagtt
     1981 agttttgatc tctggcctgg cgaaacgcta tcgctggtgg gcgagtctgg cagcggtaaa
     2041 tccactaccg ggcgggcgtt gctgcgcctg gtcgaatcgc agggcggcga aattatcttt
     2101 aacggtcaga gaatcgatac cttgtcaccc ggcaaacttc aggcattgcg ccgggatatt
     2161 cagtttattt ttcaggaccc ttacgcttcg ctggacccac gtcagaccat cggtgattcg
     2221 attatcgaac cgctgcgtgt acacggttta ttgccaggta aagacgcggc tgcacgcgtt
     2281 gcgtggttgc tggagcgcgt gggcctgtta cctgaacatg cctggcgtta cccgcatgag
     2341 ttttccggcg gtcagcgcca gcgcatctgc attgctcgcg cgttggcatt gaatccaaaa
     2401 gtgatcattg ccgacgaagc cgtttcggcg ctggatgttt ctattcgcgg gcagattatc
     2461 aacttgttgc tcgatctcca gcgtgatttc ggcattgcgt atctgtttat ctcccacgat
     2521 atggccgtgg tagagcggat tagtcatcgt gtggcggtga tgtatctcgg gcaaattgtt
     2581 gaaattggtc cacggcgcgc ggtcttcgaa aacccgcagc atccttatac gcgtaaatta
     2641 ctggcggcag ttccggtcgc tgaaccgtcc cgacaacgac cgcagcgtgt actgctgtcg
     2701 gacgatcttc ccagcaatat tcatctgcgt ggcgaagagg tggcagccgt ctcgttgcaa
     2761 tgcgtcgggc cggggcatta cgtcgcacaa ccacaatcag aatacgcatt catgcgtaga
     2821 taacattcag gcggagaata aaatggcaag agctgtacac cgtagtgggt tagtggcgct
     2881 gggcattgcg acagcgttga tggcatcttg tgcattcgct gccaaagatg tggtggtggc
     2941 ggtaggatcg aatttcacca cgctcgatcc gtatgacgca aatgacacgt tatctcaggc
     3001 cgtagcgaaa tcgttttacc aggggctgtt cggtctggat aaagagatga aactgaaaaa
     3061 cgtgctggcg gagagttata ccgtttccga tgacggcatt acttacatcg tgaaattgcg
     3121 ggaaggaatt aaattccagg atggcaccga tttcaacgca gtggcggtaa aagcgaatct
     3181 ggaccgggcc agcgatccgg cgaatcatct taaacgctat aacctgtata agaatattgc
     3241 caaaacggag gcgatcgatc cgacaacggt aaagattacc ctcaaacagc cgttctcagc
     3301 gtttattaat attcttgccc atccggcgac cgcgatgatt tcaccggcag cgctggaaaa
     3361 atatggcaag gagattggtt ttcatccggt gggaaccgga ccgtatgaac tggatacctg
     3421 gaatcagacc gattttgtga aggtgaaaaa attcgcgggt tactggcagc caggattgcc
     3481 caaactggac agcataacct ggcgtccggt ggcggataac aacacccgcg cggcaatgct
     3541 gcaaaccggt gaagcgcagt ttgctttccc cattccctac gagcaggcca cactgctgga
     3601 gaaaaacaaa aatatcgagt tgatggccag tccgtcaatt atgcagcgtt atatcagtat
     3661 gaacgtgacg caaaagccgt tcgataaccc gaaggtccgt gaggcgctga attacgccat
     3721 taaccgtccg gcgctggtga aagttgcctt tgcgggctat gcaacgccag ctactggtgt
     3781 ggtaccgcca agtatcgcct acgcgcaaag ttataaaccg tggccttacg atccagtgaa
     3841 agcgcgcgaa ttactgaaag aggcgggata tcccaacggt ttcagtacca cgctgtggtc
     3901 gtcacataac cacagcaccg cgcagaaagt gctgcaattt acccagcagc agttagcgca
     3961 ggtcgggatt aaagcccagg tgaccgcgat ggatgccgga cagcgggcgg cagaagttga
     4021 aggtaaaggg caaaaagaga gcggcgtgcg gatgttctac actggctggt cggcttcaac
     4081 cggcgaagcg gactgggcgc tatcgccact gttcgcctcg caaaactggc caccgacgct
     4141 gtttaatacc gcattttaca gcaataaaca ggtggacgac ttcctggctc aggcactgaa
     4201 aactaatgat ccggcggaaa agacccgctt gtataaggcg gcgcaggata tcatctggca
     4261 agagtcgccg tggatcccgc tggtggtaga aaaactggtg tcggcacaca gtaaaaacct
     4321 gaccggtttt tggatcatgc cagacaccgg cttcagcttt gaagacgcgg atttgcaata
     4381 agcaacgcag ggagtggaat gcttaattac gttatcaaac gcttactggg gttgattccg
     4441 acgctgttta tcgtctcggt gctggtgttt ttatttgtgc atatgctgcc cggcgatccg
     4501 gcgcgattga ttgccgggcc cgaagctgat gcgcaggtta tagaactggt gcgtcagcag
     4561 ctggggttgg atcagccgct gtatcaccag ttctggcact atatcagcaa tgctgtgcag
     4621 ggggattttg gcctgtcgat ggtgtcgcgt cgtccggttg ccgatgagat tgccagccgc
     4681 tttatgccaa cgctgtggct gaccataacc agtatggtct gggcggtgat atttggtatg
     4741 gcggcgggaa ttatcgccgc cgtctggcgt aatcgttggc cggatcgatt gagtatgacc
     4801 attgcggtgt cggggatctc gtttccggca tttgcgctgg ggatgctttt aattcaggta
     4861 ttctccgttg aactgggctg gctgcctacc gtgggagcag acagttggca gcactacatt
     4921 ttaccctccc tgacgctcgg cgcggcagtg gcagccgtga tggcgcgctt tacccgcgcg
     4981 tcgtttgtcg atgttttaag cgaagattat atgcgtaccg cgagggcgaa aggggtgagc
     5041 gaaacctggg ttgtcctcaa acacgggcta cgtaacgcga tgatcccggt agtgaccatg
     5101 atgggcttac agtttggctt tttgctcggt ggttctatcg tggtggagaa agttttcaac
     5161 tggcccggac ttggacgctt actcgttgac tccgtagaaa tgcgcgatta cccggtgatt
     5221 caggcggaaa ttctgctttt ctcgctggaa tttattctta tcaacttagt ggtggatgtg
     5281 ctttacgccg ccattaaccc ggctatcagg tacaagtaag gatgcgacta tttaactggc
     5341 gacgtcaggc ggtgttaaac gccatgccac tggtcaaacc tgaccaggtg cgtacaccgt
     5401 ggcatgaatt ctggcgacga tttcgccgtc agcatatggc gatgaccgcc gcattattcg
     5461 ttattttatt gattgtggtg gccatttttg cacgctggat cgctccctat gacgccgaaa
     5521 attattttga ttatgacaat ctgaataacg gaccttcttt gcagcactgg tttggcgtcg
     5581 attcactggg gcgcgacatt ttcagccgtg tcctggttgg tgcgcaaatc tcgctggcgg
     5641 cgggcgtgtt tgccgtgttt atcggtgcgg cgatcgggac gttgctgggc ttgttggctg
     5701 gatattatga aggctggtgg gatcggttga tcatgcgcat ttgcgatgtg ctgtttgcct
     5761 tcccgggtat tttactggcg atcgctgttg ttgcggtgtt gggaagcggc attgctaacg
     5821 tgattatcgc agtcgccatt ttttccatcc ccgcgtttgc ccgactggtg cgcggcaaca
     5881 cgctggtgtt gaaacagcaa acctttattg agtcagcacg cagtattggt gccagcgata
     5941 tgaccatttt attgcgtcat atcttgcctg ggaccgtctc gtctatcgtg gtgtttttca
     6001 ccatgcgcat tggcacctcg attatctctg ccgccagcct gtcatttctc ggcctcggtg
     6061 cgcagccgcc gacaccagag tggggagcaa tgctcaatga ggcacgagcg gatatggtta
     6121 tcgcgccgca tgtcgctgtt tttccggccc tggctatttt tctgaccgta ctggcgttca
     6181 atttgttggg cgatggttta cgcgatgcgc tggatccgaa aattaaagga tagttacgtt
     6241 tgaatattgc ttgaaagggt aatcacctca caggaaatta ttgccctaag caagtgttgt
     6301 aactttctgc tgattttgta gaatcgggta atttggttaa aaagc
//
LOCUS       DAZKGS010000223         6333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKGS010000223 DAZKGS010000000
VERSION     DAZKGS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..339
                     /gene="ydjO"
                     /locus_tag="ACHOQY_004473"
     CDS             <1..339
                     /gene="ydjO"
                     /locus_tag="ACHOQY_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFP9680431.1"
                     /translation="LRFKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSI
                     PDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKAT
                     APIQNGPLPA"
     gene            complement(343..1734)
                     /gene="tcyP"
                     /locus_tag="ACHOQY_004474"
     CDS             complement(343..1734)
                     /gene="tcyP"
                     /locus_tag="ACHOQY_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9680432.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1867..2457)
                     /gene="ydjM"
                     /locus_tag="ACHOQY_004475"
     CDS             complement(1867..2457)
                     /gene="ydjM"
                     /locus_tag="ACHOQY_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9680433.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2620..3288)
                     /gene="hxpB"
                     /locus_tag="ACHOQY_004476"
     CDS             complement(2620..3288)
                     /gene="hxpB"
                     /locus_tag="ACHOQY_004476"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9680434.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3435..3971
                     /gene="yniB"
                     /locus_tag="ACHOQY_004477"
     CDS             3435..3971
                     /gene="yniB"
                     /locus_tag="ACHOQY_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9680435.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4012..4872)
                     /gene="yniA"
                     /locus_tag="ACHOQY_004478"
     CDS             complement(4012..4872)
                     /gene="yniA"
                     /locus_tag="ACHOQY_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9680436.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4978..5268)
                     /gene="ghoS"
                     /locus_tag="ACHOQY_004479"
     CDS             complement(4978..5268)
                     /gene="ghoS"
                     /locus_tag="ACHOQY_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9680437.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5369..6298)
                     /gene="pfkB"
                     /locus_tag="ACHOQY_004480"
     CDS             complement(5369..6298)
                     /gene="pfkB"
                     /locus_tag="ACHOQY_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9680438.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1629 a         1587 c         1556 g         1561 t
ORIGIN      
        1 ctgcgattca aaaaaaatga gagtctccta aagtttcctt cgataatatc aatgcttaat
       61 atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtgt gcttgaagat
      121 attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt tttaagcttc
      181 tcactacagg cacattcatt gagccaggaa tgtattgata agttggacat ttttttcgct
      241 gacgatctgt ttaaatatga gtcagtatgc atcgccgcga tggaacattt aaaaagtaaa
      301 gcaacggccc ctatacaaaa cggaccgttg ccagcataag aattaacggt gtgccagttc
      361 ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc ttcagccact ggctggtcag
      421 cgtgccagct gtcatcgagc cactaacgtt tagcgccgta cggcccatgt cgataagcgg
      481 ttcaacggag attaacagcg ccaccagggt tactggcagg cccatcgcag gcagcacaat
      541 cagtgcggcg aaagttgcac caccaccgac accggcaacg cctgcggaac taacggtaac
      601 aataccgacc agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa ccgtaggcgc
      661 aaccatcacc gccagcattg ccggatacaa accggcgcag ccgttctgac caatggttgc
      721 accgaaagag gcggcgaaac tggcgatgga ttcaggaacg cccagacgac gcgtttgtgc
      781 ttccacattc agtgggatag acgcagcact ggaacggctg gtaaaggcaa acgtcagcac
      841 aggccatacc ttacggaagt acttcagcgg actcacgcca ttaatgccca gcagaatgcc
      901 atgcactgca aacataatca gcagaccgag gtaggacgcg acaacgaaac ttcccagttt
      961 gatgatgtct tgcaggttag aacctgcaac cactttggtc attagtgcca gaacgccgta
     1021 aggggtcaac tgcatgacca ggcgaaccag tttcatcacc cagctttgca gggtatcgat
     1081 agcggttaag acgcgttcac ctttcggcgc atcatccttc agcagtttca gcgcagctac
     1141 accgaggaat gcggcaaaaa ttaccacgct gataattgac gtcggattgg ctccggtcag
     1201 atcggcaaac gggtttttcg ggataaagga caagaccagc tgcggaacgc tcaggtcaga
     1261 gactttacca acatagttac tttcgatggc gttcagacgt gcagtttctg caccaccctg
     1321 aaccagacct tcagccgtca aaccaaacag gttggtgacc agcacaccga ccagcgccgc
     1381 aatcagtgtg gtaaacaaaa gcgtaccgat ggtcagaaaa ctgattttgc ctaactgaga
     1441 tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg aagactaacg gcataacgat
     1501 catttgcagc agttgaacat agccgttacc aacgatgtta aaccactgta cagaatcttt
     1561 aagtacctgg ctgtcagaac cataaatggt atgcagggca aggccaaaaa ccacacccat
     1621 caccagaccc accagcactt ttttcgccag actccactgt ttgtggcggg tctgagccag
     1681 cgcaaacagc agtacaacga acaccacgat gttcgcaatt aatggaaagt tcatccccgt
     1741 tctcctgatc ttattatttc tatcactgcg tagcagtcat agtgttggcg caagattaac
     1801 agaactgtga gctggctctt atattcaaat ggaattgctt attccagaaa ggtgattttt
     1861 gcctttttag tattcaacct gatgcttaat aagccgatga aactggatct gcaaggtatt
     1921 gatcatttgc gatgaccaac gaaccgcgct gttttcgggt aatgaatggg gcatccatac
     1981 cgaccagaca aataatgcca tgcagataaa acgttccagt tggttgccct tttggggaac
     2041 caggataggc aagcggaaac gccagcgaca tggccagagc aggggaacac cggcgggtgt
     2101 cagcatatcg gcaagtatgt ggcttaaata acccagcacc attccttgta gcgcatcagc
     2161 cggaatgaac cagctttcag gaaccttaag gtaaaaggtt gccagcagcg caaataccgc
     2221 cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg gcgatcggtt ttgatatcca
     2281 ctttaatcgc tgcccaagaa acgactttgg gtgatcgatg tccggtaaca aacacgtcag
     2341 gattgcggaa gggacaatat gccaccagtc accctgtgcc agcacgggcg tcagctcggc
     2401 atttttggca aataccgcac aagcaataga aaagagaagg tgaccttccg ccgtcatgat
     2461 aaaactcact aaactgtcga tacgtacagt ataggatttt tatacagtgc acggaagtgg
     2521 tgacggtgta actctttctc ttaaattgac gattaatccg tcagttgaat gcaaattcag
     2581 ataaagaacc aggggcagcg agatcactgc ccctggtcat taaccgagaa ggtcttttgc
     2641 ggtgagttct gtcagcgatg agagtttgac gtttgctaat acaaaacgtg gatcattttg
     2701 cgcttctggc gcaggaacga caatggaacg catgcgggct gctttagagg cgatcatgcc
     2761 atttactgaa tcttccagcg ctacgcaggt cagtgggtca acgcccagtt ttgctgcgca
     2821 gtcgagatat acttgcggat gcggcttgct gtaaggcagt ttttcagccg aggcgagggc
     2881 atcgaaactg tcgcgtaagt caaacatggt caacactttt tccagcatat gtagtggtga
     2941 cgcggaggcc agtcccacca ataaaccttg ttctttgcat aacgcaacgg cttcgcgcac
     3001 gcctggtaat aatggacgtg tctcttcaac cagtgaaatg gcacgggcaa taacccgttc
     3061 tactacttcc tgacggcttg gcccattcca cggttgccgg gcgtaccaaa gatcgaccac
     3121 catatcgatg cgtaaaccta aggtgtccgg cagctcgtta cgacgggaga tatccacccc
     3181 caggcttgcc atcacatcca gttcggctcg atcccataaa ggttctgagt cgataagtaa
     3241 tccatccata tcaaaaattg cagcaagaat ctgacgcggg gttgacatta caacctctcc
     3301 tttagagcat tatgtgtaag gtattaagcc gtgcttatga ttaagcgcct atcatatcgc
     3361 ctttaaagaa tgggcgaaaa cagatatcta caggtaaact aagtgaaacg acgcgtctta
     3421 atcaaggaga actcatgacg tatcaacaag ctggacgcat tgctgttttg aaaaggattt
     3481 tgggttgggt gatttttata cctgcattga tctctacatt gatttctttg ctgaaattta
     3541 tgaatacccg gcaggaaaac caggaaggca ttaatgcggt catgctcgac tttactcatg
     3601 tcatgatcga tatgatgcag gcgaatacgc cttttttgaa tctgttctgg tataactccc
     3661 cgacgcccaa ttttaatggt ggcgtgaacg ttatgttctg ggtgattttc atcctgattt
     3721 ttgtcggact ggcgttgcag gattccggtg cccggatgag tcgccaggcg cgcttcttgc
     3781 gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa aggggaagag gggctaacgc
     3841 gtgaacaaat tgagtcccgt attgttgttc cgcaccatac tattttcctg cagtttttct
     3901 cactatatat cctgccggtt atttgtattg ctgcaggtta tgtgttcttt tctctgcttg
     3961 ggtttattta atcaaaaggc cgcacggagc ggccattatc ctcaacccat atcatgctgc
     4021 taataatcta tccaatgact gctgagcaat aaccaaatgc tgaccgccaa ataatcttgc
     4081 acgatttaac agcgtgtaga gttggtaaac cggttgacgt tcaaggaaat cggcaggtag
     4141 cggggatact gactgatagc cgtcatagat ttgtggcggc tgttcagtat gcagcggtaa
     4201 catcgccagg tcgcactctc ggtcacccca gtagcaggca gggtcgaaaa tgtacgggcc
     4261 atccggaccc agcgcacagt tgccggacca taaatcgccg tgcaacagag aaggctgcgg
     4321 ttgatgcgag gccagacgct gctgaatatg ctcgacgatg gcgtcgatat tgccgaaagc
     4381 gatccctttc tctgctgcca gttccaactg ccagccaatc cgttgttcag caaaaaacgt
     4441 tgaccagcga cgttgccagg tgttgggctg tggagttgtg gagagcgagt tatcaaaatc
     4501 gaggccaaat tgtggttggt cactccattg atgtaaacgc gcaatttgct gaccaagaat
     4561 aaatgcgcta tgcgcatcca gcggacgagg tgggagataa tccatcacca gaaaactgta
     4621 gtcacggtca gcgcctactg cccaaacctt aggcacggtg acggttttac tacgcgacag
     4681 taactccagt tggtcggctt cggcggtaaa accgggaagc agttcccttt catcacattt
     4741 gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg gcatggactt ctccgccagg
     4801 cagttcatta cgcagttcga tttcgccttc acctaactgc tcgctcaaaa gacgactgat
     4861 tgcctgccac atgatgtctc tcccatgttg tctgccagat cataaggtta gcgcaagaat
     4921 gcggtgtaaa aacacgagct aacgcacacc acagactgat tattgcccaa aaaccattta
     4981 ttttctgttg ctattccatt cctcccaggt cgtgatggtg atttcaggat ctttgccggt
     5041 tgcactttgg gtaagccccg aaaccagttc tctaatttct tcttcacttt gggtactgat
     5101 aagtccaaaa gtgttagtcc ccagctcatg gatatttccc tcatcatccg tcatggtgag
     5161 caaaaaaccg tcgcgagtca ggtaattatt caactcgtta atttcagtca atgtatcctc
     5221 atgcaacatg acggttatga cataacggac aagatcgcca ctggccatag ttcacccctt
     5281 tgttatcaca tgacttttga gcatagtcgg agaaacgcgt tgccgacagg ttggtggtga
     5341 ttcccccaat gctgggggaa tgtttttgtt agcgggaaag gtaagcgtaa attttttgcg
     5401 tatcgtcatg ggagcacaga cgtgttccct gattgagtgt ggctgcactc cccgcagcta
     5461 cgccaaaacg gaccatctct tcaagagagg cattttctgc cagtttcagt gtcatcgcgc
     5521 cgaccatgct gtcaccagcg ccaacggtac tctggctttt caccggtggt ggcaccacct
     5581 gaatgcagtt ttcactatca acacccagcg ctccttgtgg accaagggaa acgacaaccc
     5641 gtttggcctt gccgctatta acgatttcct gcgcggcttt gcggacatcg tctggctggg
     5701 tgagttcgcg attcaccagc gcactgagtt ctttttggtt aggcttaacc aactcgatgt
     5761 taccaatcgc cagtgctgca cttaacgctt cgccagaact gtcgatgatg cagcggatcc
     5821 cttggttttg cgcagcggaa atcagttggg ttaatttttc cagcttcaca cctggcggca
     5881 ggcttccgct tatgaccagg atggccccgg attcaatttc cagaacttgc tcttcaagct
     5941 ggcgaaactc atcttcattt aatgccgcgc ctggcataac aaaacgatac tgctcaccgc
     6001 ttgcttccac atgtacgtgt aaattctgcc gggtccagtc tttggcttct acagtagcga
     6061 cggggacatt ttcatccgcc aacagagaaa ccaggtgttc gccggtcgcg ccacccgccg
     6121 ggaagatcgc tgtggcactg cctccaagat gggcaatggc gcgggcgacg ttgatgccgc
     6181 cgcccccggg ttcgaacacc ggtgcggtac agcgcagttt tccttcggga taaatttgcg
     6241 gggtaattgt tgcgctatcg agagagggcg caagtgtcaa cgtatagata cgtaccatca
     6301 tttcctccta taggctgatt tcagtctggc acc
//
LOCUS       DAZKGS010000224         6300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKGS010000224 DAZKGS010000000
VERSION     DAZKGS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..312)
                     /locus_tag="ACHOQY_004481"
     CDS             complement(<1..312)
                     /locus_tag="ACHOQY_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680439.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCALIACFIFLFLVLSIN
                     IF"
     gene            453..2885
                     /gene="hsdR"
                     /locus_tag="ACHOQY_004482"
     CDS             453..2885
                     /gene="hsdR"
                     /locus_tag="ACHOQY_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFP9680440.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2952..4421
                     /locus_tag="ACHOQY_004483"
     CDS             2952..4421
                     /locus_tag="ACHOQY_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9680441.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4421..6190
                     /locus_tag="ACHOQY_004484"
     CDS             4421..6190
                     /locus_tag="ACHOQY_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9680442.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TRIKTAQQTQLHLADALTDAAIN"
BASE COUNT         1946 a         1466 c         1429 g         1459 t
ORIGIN      
        1 aaaaatatta attgacaaca ctaaaaacaa aaatataaaa cacgcaataa gtgcacacca
       61 ggcagcacaa ctattcaacc tgtttttatt actgaactta acaaaatacc ccatatattc
      121 tgtaatgcga ttttcttcca ctaatgcata aagaagaata ccaacaaagc acagagataa
      181 aataatatct ttccacagat atttataagg gagaagtata aaaccaagaa atagataaat
      241 tagcagaatg acatctaatg ccttagtaca aaaatattgt ttcactcctt gcaagaaccg
      301 aacattctcc atatgaatcc ttatcctact attgatgaaa ctcaagaata cctttaaaac
      361 agtgacttaa cgctgataat actctttgaa cagatgaatt catctatttt tctgtgtaga
      421 atttgcgcat cgcatttaga tgagaacacc ccatggcgga actgaaccta agtaacctga
      481 cggaagcaga catcattacc aaatgcgtta tgccagccat tctcaatgca ggctgggaca
      541 acacaacgca aatcagacag gaggtcaaac tccgggacgg taaagtcatc gtacgtggta
      601 aagttgcggc acgcagaacg gtaaaatctg ctgacatcgt gctgtatcac aaaccaggca
      661 ttcccttagc cgtgattgaa gcaaaagcca acaaacatga aattggcaaa gggatgcaac
      721 agggcattga atatgcgcgc ctgctggacg ttccttttgt ttttgccacc aacggggatg
      781 gctttatctt ccgcgatgcc accgcagccg aaggtgaatg ccttgaaaag caaataacgc
      841 tggatgactt cccctccccc gctgaactct ggcaaaaatt ctgcctctgg aaaggataca
      901 cacaagctca gcttccggtg attactcagg actattacga cgatggcagc ggtaaatcgc
      961 cacgttatta ccagcttcag gcaatcaaca aaaccattga agccgtctcc aacgggcaaa
     1021 accgcgttct gctggtcatg gcgaccggaa cagggaaaac ctataccgca ttccagatca
     1081 tctggcgcct gtggaaatca aaaaataaaa aacgcatttt gttccttgcc gatcgcaaca
     1141 ttctggtcga ccaaaccaaa aataatgatt tccagccatt tggtacggca atgaccaaag
     1201 tcagcggacg caccattgat cccgcttatg aaattcacct cgcgctctat caggctataa
     1261 ctggcccgga ggaagaccaa aaagcgttta aacaagtcgc accagatttc ttcgatctga
     1321 tcgtgatcga cgaatgccat cgcggcagcg catccgaaga cagcgcctgg cgagagatcc
     1381 ttgattactt cagttccgcc acccaaattg gcttaaccgc cacgccaaaa gagacgcatg
     1441 aagtctccag cacggattac ttcggcgatc cggtttacgt ctactcgcta aaagaaggga
     1501 tcgaagacgg cttcctcgcc ccttataaag ttgtccgtgt tgatattgat gttgatctgc
     1561 aaggctggcg cccaaccaaa gggcaaactg acttaaacgg cgaagtgatc gacgatcgta
     1621 tctataacca gaaagatttc gatcgcacga tggtaatcga cgaacggaca gaactggttg
     1681 ccagaaccat taccgactac ctcaagcgca ccaacccgat ggataaaacc atcgtcttct
     1741 gtaacgacat cgatcatgca gaacgtatgc gccgcgccct ggtgaatctc aacccggagc
     1801 aggtgaaaaa gaacgacaaa tacgtcatga aaatcaccgg cgatgatgaa attggcaaag
     1861 ctcagttgga taacttcatc aacccgaaaa aagcgtaccc ggttatcgcg accacttcag
     1921 agctgatgac cactggtgtg gatgctaaaa cctgcaaact ggtagtactg gaccagaaca
     1981 tccagtcgat gaccaaattc aagcaaatta tcggtcgtgg tacacgcatc gacgaacgtt
     2041 acggcaaact ctggtttacc atcctcgact ttaaaaaagc caccgaactg tttgccgatg
     2101 agcgtttcga tggcattccc gaaaaagtca tggataccac accagaggat atcgccgatc
     2161 cagaatctga ttttgaagag aaactcgaag aaatcagcga acatgacgac gaacaggtaa
     2221 caggcgttga tgaaccgcct gcgccaccat accaggttac cgataccgat gatgtcggcc
     2281 cacttccgga agaagacgag aagaaaatcc gcaagtttca cgtcaacggt gtagcggtgg
     2341 gcgttattgc gcagcgtgtt cagtattacg atgccgacgg taaactggtt accgaatctt
     2401 ttaaagatta cacccgcaaa acactgctca aagaatatgc ctcgctggat gactttaccc
     2461 gcaagtggca ggacgccgat cgcaaagaag cgatcattca cgagctggag caacagggga
     2521 tcatctggga agtactggca gaagaagtcg gtaaagatct cgacccgttc gacatgcttt
     2581 gccacgtagt gtatggtcag ccgccgttaa cccgcaaaga gcgcgccgag aacgtgcgca
     2641 agcggaacta cttcacaaaa tactctgaag cagcgcaagc cgtgctcgat aatctgctgg
     2701 ataaatacgc cgatgcgggc gtacaggaga tcgaaagtat tcaggtgctg aaacttaaac
     2761 cattcgacag catgggcact ttaccggaga ttattaaaac cggatttggc gaccgtaacg
     2821 ggtataatca ggcgctcagc gagctggaaa acgaaatcta ccaattaccg ccccgctctg
     2881 cttaacaact ctcttttgtg ccttctgcaa ccaacatgtt gcagaaggcc ttgtttttca
     2941 tggaatacaa catgtctatc agttcagtaa tcaaatcatt acaagatatt atgcgcaaag
     3001 atgccggtgt ggatggcgat gcgcagcgtc tcggtcagct ctcctggctg ctgtttttga
     3061 aaatcttcga tgcccaggaa gaggtgctgg aactggagca ggataactat caatatccga
     3121 tcccacagcg ttatttatgg cgcagttggg ccgcaaacgc tcagggcatt accggtgatt
     3181 ccctgctgga attcgttaat gatgatctgt tcccggcgtt gaaaaacctc actgcgccta
     3241 tcgataaaaa cccacgcggc tacgtggtga agcaggcgtt cagcgatgcc tataactata
     3301 tgaaaaacgg tacgctactg cgccaggtga tcaacaagct gaacgaaatt gactttacca
     3361 gcgccagcga acgccatctg tttggtgata tttacgaaca gatccttaaa gatctgcaat
     3421 ctgcgggcaa tgctggcgaa ttctatacgc cacgcgccgt cactcgcttt atggtggatc
     3481 gcgttgatcc gaaactcggc gaatccatta tggacccggc ctgcggtacg ggcggttttc
     3541 ttgcctgcgc atttgatcat gtaaagaaca aatacgtgaa gagcgtcgcc gatcatcaga
     3601 cgctgcaaca acagatccac ggtgttgaga aaaaacagct tccgcacctg ctggcgacca
     3661 ccaatatgct gctgcacggc attgaagtac cggtacaaat tcgtcacgac aacaccctga
     3721 acaaaccgct ttcctcctgg gatgaacaac tggatgtcat tgttaccaac ccgccgtttg
     3781 gtggcacgga agaagacggt attgagaaga actttccggc agagatgaaa acccgcgaaa
     3841 cggcggatct gttcctgcaa ctgattgtgg aagtactggc gaaaaacggt cgcgcggcgg
     3901 tggtattgcc ggatggcaca ctatttggcg aaggcgttaa aaccaaaatc aaaaaactgc
     3961 ttaccgaaga gtgcaacctg cataccatcg tgcgtttacc gaatggggtg tttaacccct
     4021 ataccggcat taaaaccaac ctgttgttct ttaccaaagg tcagccaacc aaagagattt
     4081 ggttctatga gcatccgtat ccggcgggcg taaaaaacta cagcaaaacc aagccgatga
     4141 agtttgaaga gtttcaggcg gagatcgact ggtggggtaa cgaggccgat ggttttgcca
     4201 gccgcatcga gaatgagcaa gcgtggaaag tcagcattga tgacgtgatt gcgcgtaact
     4261 tcaatctgga tattaaaaac ccacatcagg cggaaaccat tagccatgat ccggacgaac
     4321 tgttagcgca gtatgcaaaa cagcaggcgg agatccagac gctgcgtaat caactgcgcg
     4381 atattcttgg cgctgcgctg tctggcaagg aggttaacta atgagtgtgg aaaagctgat
     4441 cgttgatcat atggaaacct ggacctcggc gttgcaaacc cgttccaccg ccgggcgcgg
     4501 cagctccggt aagattgatt tgtatggtat taagaaattg cgtgagctga ttctggaact
     4561 ggcggtgcga ggtaaactgg tgccgcagga tccgaacgat gaaccggcgt cggagctgct
     4621 gaagcgtatt gcggcggaaa aagcagagct ggtgaagcaa ggtaaaatta aaaagcaaaa
     4681 accgctgccg gaaattagcg aggaagagaa gccgtttgaa ttgccggagg ggtgggaatg
     4741 gactcgatta attaatcttg gtatatgggc tttagggtct ggttttccaa atgtggtaca
     4801 gggaagcact gataaagaaa ttttgatgtg caaagttagc gacatgaatc ttgaaggtaa
     4861 cgagaaattc attttttcga caaaaaatac aatttcaaaa gacctagctg atgaatataa
     4921 aattaaaatc tcagaacccg gcaccatcat tttccctaaa attggcggag caattgcaac
     4981 taataaacga agaattttag ttcaggatac agctattgat aacaactgtc ttggaataaa
     5041 accatgtgat gcaatatcgg gggagtggtt ctatctaatc cttaatactc tcgatatgtc
     5101 gaagtatcaa tcaggaacct caatacccgc gataaatcaa agtgtcatag gtagtatacc
     5161 aattgcgctt ccttctttaa aaatgcaaga gaagattgtt tcttatgtca taaccctaat
     5221 gtccctttgc gaccaactgg aacagcaatc cctgaccagt ctggacgcac atcagcaact
     5281 ggttgaaacc ctgttgggaa cacttacaga cagccaaaac gccgaggaac tggctgaaaa
     5341 ctgggcgcgt attagcgagc atttcgacac cctatttacc accgaagcca gcgtggatgc
     5401 gttaaaacag accattctgc aactggccgt aatgggcaaa cttgtgccgc aggatccgaa
     5461 tgacgaacca gcctctgaac tgctcaaacg aattgcgcag gaaaaagctc aactggtgaa
     5521 agaaggaaaa ataaaaaaac aaaaaccgtt gccgccaatt agcgatgagg aaaaaccgtt
     5581 tgaacttccg gaagggtggg agtggtgtaa atttggttta acatcagagt ttataaatgg
     5641 cgatcgtgga agtaattacc ccaataaaaa tgagtatgtt tctcaaggta ttccttggat
     5701 caatactgga catatagaaa aaaatggtac acttacggtt acagaaatga attttataac
     5761 cgaaggaaaa tttaatgaat taagaagtgg gaaaattcaa aaaggtgatt tggtttattg
     5821 cctacgagga gcaacttttg gtaaaactgc ctttgtaatc ccctatgaaa caggagctat
     5881 tgcctcatca ctaatgatca ttagaccatt tatcacagaa atgggagggt atatttataa
     5941 ttatttaacc agtccatttg ggcgctcaca gatttaccgc tttgataatg gctcagcaca
     6001 acccaaccta tcagcaaata gtgtaatgtt gtattcattc ccatgcccac cattaactga
     6061 acaatataga atattttcac aagtaggttt acttcatgaa ctatgcgata agcttaaaac
     6121 tcgtataaaa acagcccaac aaacccaact gcaccttgca gacgcactca ctgacgcggc
     6181 gataaactaa ttttctcgcc ctcaaaaaac caataaagcg gctaaatggc cgctttattc
     6241 acatcaccaa aaattatatt tcacgctaat tttcatctta ttttcattaa tttttagctg
//
LOCUS       DAZKGS010000225         6187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKGS010000225 DAZKGS010000000
VERSION     DAZKGS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..272)
                     /locus_tag="ACHOQY_004485"
     CDS             complement(<1..272)
                     /locus_tag="ACHOQY_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP9680443.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRV"
     gene            complement(269..1939)
                     /gene="menD"
                     /locus_tag="ACHOQY_004486"
     CDS             complement(269..1939)
                     /gene="menD"
                     /locus_tag="ACHOQY_004486"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9680444.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2028..3323)
                     /gene="menF"
                     /locus_tag="ACHOQY_004487"
     CDS             complement(2028..3323)
                     /gene="menF"
                     /locus_tag="ACHOQY_004487"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9680445.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3402..3707)
                     /gene="elaB"
                     /locus_tag="ACHOQY_004488"
     CDS             complement(3402..3707)
                     /gene="elaB"
                     /locus_tag="ACHOQY_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9680446.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3762..4223)
                     /locus_tag="ACHOQY_004489"
     CDS             complement(3762..4223)
                     /locus_tag="ACHOQY_004489"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9680447.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4288..5205
                     /gene="rbn"
                     /locus_tag="ACHOQY_004490"
     CDS             4288..5205
                     /gene="rbn"
                     /locus_tag="ACHOQY_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9680448.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5393..>6187
                     /locus_tag="ACHOQY_004491"
     CDS             5393..>6187
                     /locus_tag="ACHOQY_004491"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFP9680449.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALG"
BASE COUNT         1549 a         1661 c         1471 g         1506 t
ORIGIN      
        1 acccgtccgc caagcgagta ccccaccagc cagaagtcaa ggatgttgta actaaccaag
       61 gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca
      121 ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct
      181 tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt
      241 tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct
      301 ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg
      361 tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt
      421 tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac
      481 gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca
      541 ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt
      601 aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc
      661 ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt
      721 aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa
      781 acaattgccc ctgttcaggc agatagtcgc tgatgcgatg cgccagttgc gcttcgccaa
      841 acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa
      901 cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa
      961 ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc
     1021 agtactcttc gggttcacag cttgcctgcc attgcaggag ccgtttcccc gtcaggctgc
     1081 ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta
     1141 accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg
     1201 gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc
     1261 cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt
     1321 ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct
     1381 gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt
     1441 tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc
     1501 aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg
     1561 cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc
     1621 gatcggcggt taagagaatc agtttttctc cggttaaccc ggcttcaatc agtgccggat
     1681 agagatttgc caccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt
     1741 tcgccagccc cagcgccaga tgccccagtc cacgctcatc gaaatgggtg tggtgaatga
     1801 atgcggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcct ggggcgatac
     1861 agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt
     1921 taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc
     1981 tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta ttccatttgt
     2041 aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg
     2101 gaaccacgga caatgcccgc gccagcatat agccgcacga cattgccgct gatttttgct
     2161 gaacgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg
     2221 taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt
     2281 aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg
     2341 ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc
     2401 ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc
     2461 accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc taactgctgc
     2521 gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt acgcagcgct
     2581 ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca
     2641 aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc
     2701 gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg
     2761 agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg
     2821 tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc
     2881 accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg
     2941 aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa
     3001 agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt
     3061 tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg
     3121 cccaggacgg ctgcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt
     3181 tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg
     3241 ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc
     3301 agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg
     3361 ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac
     3421 agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg
     3481 acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact gtctgaagcc
     3541 tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca
     3601 tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc
     3661 agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat
     3721 ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg aattacctcg
     3781 cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa
     3841 ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca
     3901 ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gctgctgccc cactttttcg
     3961 ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca
     4021 cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccgag aatatggcga
     4081 ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca
     4141 aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga
     4201 tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg
     4261 gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcggccggt
     4321 gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag
     4381 agcggtcttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc
     4441 tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt
     4501 ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt
     4561 tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg
     4621 accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg
     4681 cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa
     4741 gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct
     4801 ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca cgctggaaga tggaaggcag
     4861 attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc
     4921 gataccggcc cctgcgatgc cgcacttgac ctggctaaag gtgtcgatgt catggtgcac
     4981 gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca
     5041 cgccaggctg cgacactagc ccgtgaggct ggagtcggta agctaatcat tacccacgtc
     5101 agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc
     5161 ccggcgactg aactagcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta
     5221 aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc acccacacgg
     5281 gcatattgag cgatttttga tatttattca tctccttacg cactatattc agagcaataa
     5341 gttgcctctt tttatcttca ttcagcacaa cctttgcaac taaaaggagc ccatgatggt
     5401 tacagttgtc agcaattatt gtcaattatc tcaaacccaa ctcagtcaga catttgcaga
     5461 aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga
     5521 tgaagaaagt attgagttac tgcataatat tgcgttaggt tatgatgaat ttggaaaaaa
     5581 agctgaagat attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat
     5641 ccgacttatt aaaaatgcct gtttaaaatt atataatttg gcacataccg caaccaactc
     5701 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
     5761 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
     5821 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatgata caaacttctg
     5881 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
     5941 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
     6001 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
     6061 agtccagtta ttccacataa atactggcgg acactggata ttagtttcgc tacagaaaat
     6121 agtaaataaa aaaaataata aactacaaat aaaatgcgtc atattcaact cattgcgtgc
     6181 actaggc
//
LOCUS       DAZKGS010000226         5926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKGS010000226 DAZKGS010000000
VERSION     DAZKGS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..235)
                     /locus_tag="ACHOQY_004492"
     CDS             complement(<1..235)
                     /locus_tag="ACHOQY_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9680450.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEI"
     gene            complement(268..564)
                     /locus_tag="ACHOQY_004493"
     CDS             complement(268..564)
                     /locus_tag="ACHOQY_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9680451.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            complement(703..930)
                     /locus_tag="ACHOQY_004494"
     CDS             complement(703..930)
                     /locus_tag="ACHOQY_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680452.1"
                     /translation="MQNLDEPIKGVGIPEVAKACGVSERAVYKWLKNGFLPKTEFFGK
                     TKYASKIEEISGGKYQASEMLEISKKNLLAA"
     gene            1009..1716
                     /locus_tag="ACHOQY_004495"
     CDS             1009..1716
                     /locus_tag="ACHOQY_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9680453.1"
                     /translation="MVQNEKVRKEFAQRLAQACKEAGLDEHGRGMAIARALSLSSKGV
                     SKWFNAESLPRQEKMNALAKFLNVDVVWLQHGTSLNGANDEDTLSFVGKLKKGLVRVV
                     GEAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKV
                     FPGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILK
                     QSRHLDDIEAREWLKSS"
     gene            1886..2788
                     /locus_tag="ACHOQY_004496"
     CDS             1886..2788
                     /locus_tag="ACHOQY_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680454.1"
                     /translation="MPNLPMGGSQGELPLVPTVEVVADGIPMGVLNDGTPYLTLYGLA
                     KLCGIDEAPLRMFTSNWETEKNKPRGQKVAAYLAEKGFNNVDRLYTRVINSSNVETHA
                     YPDYVCMAVLRYYALDATNFDRSVAIGNFVRLAEYTLKRMIYEKANYNPNAAIDVSFE
                     NYRARIKLNNQIPTTHFAVFREIADIAMTLIGNGFPMDDTTSLDGSVGNHWGKYWTKN
                     NLSERFGDRVQYQHIFPENYRQSAANKYIKAWIYPIESLGLFRKWLHDNYAMEKLPKY
                     LGNKKISNITELLEAVKKPALPNQ"
     gene            3615..3683
                     /locus_tag="ACHOQY_004497"
                     /pseudo
     CDS             3615..3683
                     /locus_tag="ACHOQY_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            3742..4212
                     /locus_tag="ACHOQY_004498"
     CDS             3742..4212
                     /locus_tag="ACHOQY_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680455.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            4356..4667
                     /locus_tag="ACHOQY_004499"
     CDS             4356..4667
                     /locus_tag="ACHOQY_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFP9680456.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            4743..4877
                     /locus_tag="ACHOQY_004500"
     CDS             4743..4877
                     /locus_tag="ACHOQY_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680457.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            4862..5014
                     /gene="kil"
                     /locus_tag="ACHOQY_004501"
     CDS             4862..5014
                     /gene="kil"
                     /locus_tag="ACHOQY_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9680458.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            5090..5260
                     /locus_tag="ACHOQY_004502"
     CDS             5090..5260
                     /locus_tag="ACHOQY_004502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680459.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            5271..5876
                     /locus_tag="ACHOQY_004503"
     CDS             5271..5876
                     /locus_tag="ACHOQY_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFP9680460.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT         1839 a         1217 c         1341 g         1529 t
ORIGIN      
        1 taatctcgct aagttgagaa tcggtgattc tgtccattgg tttattccac ccataggttt
       61 tacgcagaat ggcaagcagc actttaaact gtcgcttggt cagatctgcg cctgaataag
      121 cctcaagcag catatttgat agtctggcgt aaccatcatc gagatctgcc acattacgct
      181 cctgttcggc aaagttacct ctgccgaagt tgagtatttt tgctgtattt gtcataatga
      241 ctcctgtgga ttgatccagt aattccctca gaattgcata tcaatttgct tagagtcccc
      301 ggcggccacc ggggattttt tctttgtgat ttcatcaagc gcatacttaa aagctctgct
      361 aatcggactg atgtctgatg ccatgccaaa agcacacaag accgaagcta taaacctcca
      421 gtctgttctg cttatcttcg attcatgaca gccaatcatc tttgccagac cgcgctgggt
      481 aagcgttgac aggttgatga gtaaatcagt ttcagcgcga tcaatttctc gctgtgatag
      541 tttgctgtaa cttgtttgtt ccatttctta agatttccaa tagtgaatag ctagttgaaa
      601 ggtatgcgtg gaaacgcata tggccttagt tggtcagata tattgggact cgctttgtca
      661 gcgacgtagg acgaatgtcc attgtgaaaa tagcggtgtt acttatgcag ccagaaggtt
      721 ctttttgctt atttcaagca tttcgcttgc ttgatatttg ccaccagaaa tctcttcgat
      781 ttttgatgcg tatttagttt tcccaaaaaa ctcagtctta gggaggaagc cgtttttgag
      841 ccacttatag acagcccttt cgctaactcc acaagccttc gcaacttcag ggatgccgac
      901 acctttaatc ggctcatcaa gattttgcat aggaatatcc tttttcgtac tttcagtacg
      961 tattatggtt gaactgaaag tttttgcaag tgctttagta tcgtactcat ggttcagaat
     1021 gaaaaagtgc gcaaagaatt cgcccagcgg ctagcgcaag cctgtaaaga agctggtctt
     1081 gatgaacatg gtaggggaat ggctatagcc cgtgcccttt ctctttcgtc caaaggcgtt
     1141 agcaaatggt ttaatgctga gtctttaccg cgtcaggaaa aaatgaatgc gcttgcgaaa
     1201 tttctaaacg ttgatgttgt ttggcttcag cacggcactt cgttaaatgg agcgaatgat
     1261 gaagatactc tttcatttgt tggcaaatta aaaaaagggt tagtgcgcgt ggttggtgag
     1321 gcaattcttg gtgttgatgg tgccatcgag atgaccgaag agcgcgatgg gtggctcaaa
     1381 atttatagcg atgatccaga tgcctttggt cttcgtgtga aaggagacag catgtggccc
     1441 agaataaaat caggagaata tgtactcatt gagcctaaca ccaaagtatt cccgggggat
     1501 gaggtgtttg tcagaactgt tgaaggacac aacatgatca aagttcttgg ctatgacaga
     1561 gacggagaat accaatttac aagcatcaac caagatcata ggccaataac gttgccttat
     1621 catcaagtag caaaggtgga gtatgtagct ggtattctga agcaatctcg ccatctggat
     1681 gacatcgagg caagggagtg gctgaaaagt tcgtgacttc atcgtcacat agctggtaac
     1741 cagtggcctg aagagacgtt tgggtaacca caatacaacc ttgttgtcgc aaaaagatcg
     1801 catatgcgat tgtttattag aaatagaaat cgccatatag gcgaatagtc gatcgcggcg
     1861 cataacctaa ggaaatcaat aacatatgcc aaatttaccg atgggcggat cgcagggtga
     1921 attaccctta gtacctacag tagaagttgt tgctgatggc attcctatgg gggtgcttaa
     1981 tgatggaacc ccttatctaa ctctttacgg tttagccaaa ttatgtggca tagatgaagc
     2041 acctcttcga atgtttacat ctaactggga gactgaaaaa aataagccaa gaggacaaaa
     2101 agtagctgca tacttagctg agaaaggatt taacaacgtt gaccgactgt atacccgcgt
     2161 cataaatagc tcaaacgttg aaacacatgc ttatcctgac tatgtttgta tggctgttct
     2221 taggtactat gccctcgatg ccacaaattt tgatagatcc gttgcaatag gaaactttgt
     2281 tcgcttagct gaatacacgc tcaagcgaat gatctatgaa aaagcaaact ataaccccaa
     2341 cgctgccata gacgtttcat tcgaaaacta tagagctcgt ataaagctaa acaaccaaat
     2401 accaacaact cattttgctg tattcagaga aatagcagat attgcaatga cgttaatagg
     2461 aaatggcttt cctatggatg ataccacgtc actagacgga agcgttggaa atcactgggg
     2521 aaagtattgg acaaaaaaca acctttccga gagatttggt gatagagttc aatatcaaca
     2581 cattttccct gaaaactaca ggcaatctgc agccaacaag tacataaaag cctggattta
     2641 ccctatcgaa tcattggggc tattcaggaa atggcttcac gataactatg ccatggaaaa
     2701 actacccaaa tatcttggaa acaaaaaaat cagtaacata actgaactat tggaagccgt
     2761 caaaaaacca gcattaccaa accagtgata aaattattta cgctacagca acccggactc
     2821 tgcgccgggt tttctttgcc tcacgttcgc ccacctaaaa aacataacca attgtattta
     2881 ttgatgtaac tcgctaaacc atgcagttat gatccctgcc gcataacctt catcagccac
     2941 attttcaaaa ataaatttcc ttatatatca gaatcatact tcgtagagtt aataaatcac
     3001 caaaattcgt accaatagtt cttgataatg tcgaactatt ggttcattat tatcgtcgtc
     3061 agcaggacgc attactcacc agggcggtga atatacaacg attcgaatat gaatctacgg
     3121 cgctgacaaa gcgcaataac caaagtgaac tttggggtgt ggtgaagggt tcatggacgg
     3181 gaatatgtcg cacgtaaagc ggcgaggcct gcgggactat tgccgaattg aagtaggccg
     3241 aaacaggtcg aaatgggtct cccacctacc acaccaccaa agttcatcag gaggtctata
     3301 tgacacgcag aactcagttc aaaggcaatt cacgttctcg tcgtcgtgag cgtttaaagg
     3361 caaaggcatt agctaacggc gtactggccc gcgaagaagc aataagttca gaagtattac
     3421 accgcccaac tctaagtaga gcgcagattc aggctaaagg tactcacgaa acgcctgatc
     3481 gcatagaaga cgctaagcca attaagttca tggcacagga cgtgatctgg cagcaggaag
     3541 aatacagacg caatctggag cgagcggcca ttgtgtacgc gaatgagttt ggacataagc
     3601 aaccagaaac tggtgtatgt cttccaaatg tagctcttta cgcggcaggc taccggaaat
     3661 ccaaacaact gacagcaaga tgacttgtgt tggtcgccag aaaatgaaat taggcagcaa
     3721 accacttatt tgaggtgaga tatgacaaaa tcatggagcg taccttttcc tgaatcagaa
     3781 actgaacatg atggaatgcc tgttttctgg agattccagg cgacagttga agaagatggg
     3841 ataaaaatat tcgcacttca atatatagct tttcatcaga cagagcatta tgcatggttg
     3901 gttcctgcgc attggattgt taattttaaa ccagcaccaa atcagtggtt acaggaatgg
     3961 aaacaaagga gaaatagata tgcaattaag aaagtagcaa aaaatgcaga aagatctttt
     4021 gcattcccaa cgaagaaact tgctattgaa agtttattgc gccggaagaa ataccattta
     4081 atgagaataa aacaagattt ggctgttgta tcaactcttg ttgatgggat gaaaaatatt
     4141 gatacatcaa caccagatat tgaatataac tttggacaca accaagaaac agaaaattgg
     4201 gtattttatt agtacgaata agcactgtgt attcattcca acgagtgaat acacggagca
     4261 atgtcgctcg taactaaaca ggagccgact tgttctgatt attggaaatc ttctttgccc
     4321 tccaatgtga gggcgatttt tttgacggag gaaatatgaa attacgtgtc tggcatatcc
     4381 cgcaagtacc tatgaaaccg ttcattgcag aagtggcaag tgttgaagag ggtgttcgcc
     4441 tgatgggcgc actggctgat tatgacgcct ttcagtatga caacaacatc aagcctgatt
     4501 actgcaatgc taacggcctt gagatgtggg atgagagcct taccgatgaa gatttatcag
     4561 aggtggggct tactgatcgc tgggtggatt ggtacagcga atgccaatgt tacgacgacc
     4621 cacgtaaata tctcgaaagc ctgaaagaag aaacatcagc cgcctaagcg cggctttacc
     4681 gcataccaat aacgcttcac tcgaggcgtt ttcgttatgc aatcaaatat aaggagttac
     4741 ccatgatgca ctttcagctc gcgggtagcg gcgtcatgtc cgctttctac ccgcacgaat
     4801 ctgaattatc acgccgagtt aaacaattaa ttagagcagc aaagaaacaa ctggaggcgt
     4861 tatgcgcaat gaaatagcca ttaatcacca gatgcttcgt gcggcacaaa acaaagcagt
     4921 aatagccaga tttattggtg attcaaaaat gtggcttgaa gcaaataaag cgatgaaatc
     4981 agctatcaac cttccgtggt atcgcaggaa atgagtttca cagataactg gtcagacgaa
     5041 gaattcattc gtcagatgaa agaattaatc ggtaacgaag gagatattca tgtcacttgc
     5101 aaccacagtg aaggagagca agttacagag acgcatgtac acgcagcagg cgttaatgta
     5161 tcgccagaag ggagatcgtg aaggtgttcg cgtattttta aatgcggcaa agactgaagt
     5221 attaaatcag cgttatttcc ttgggccatg cccattctga gaacaaacat atgagcaaag
     5281 acttttacgc aagactggca gctattcagg agaacttgaa cgcgccaaag aatcagtaca
     5341 actcattcgg taaatataaa tacagaagct gcgaagacat tcttgaaggc gttaagccgt
     5401 tactgaatgg cctgttttta tcaatcagcg atgaagttgt gttgattggt gatcggtatt
     5461 atgtgaaagc cacggcaact attaccgatg gcgaaaacag tcatacggca accgctcttg
     5521 cacgagagga agaaagcaag aaaggaatgg attctgcaca agttacggga gctacaagct
     5581 cttatgcacg caagtattgc ctcaatggtt tgttcggcat tgatgatgcg aaagatgcag
     5641 ataccgacga gcataaacat cagcagaacg cagcagcaaa gcagtcaaaa ccatcaccta
     5701 cacctgaaca ggttctaaaa gcattcactg acgcagcatt gcagaaaaac accgtggaag
     5761 agcttaaaca ggcgttcgcc aaagcgtgga agatgctcga aggcacaccg gagcagcaca
     5821 aagcgcagga cgtttacaac atcagacgag acgaattaga aggagctgct gcttaatggc
     5881 acattcgatt actgtaagac taaacaagcc cgcaagagag tttcag
//
LOCUS       DAZKGS010000227         5872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKGS010000227 DAZKGS010000000
VERSION     DAZKGS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..622)
                     /locus_tag="ACHOQY_004504"
                     /pseudo
     CDS             complement(89..622)
                     /locus_tag="ACHOQY_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(627..791)
                     /locus_tag="ACHOQY_004505"
     CDS             complement(627..791)
                     /locus_tag="ACHOQY_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680461.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(788..1521)
                     /locus_tag="ACHOQY_004506"
                     /pseudo
     CDS             complement(788..1521)
                     /locus_tag="ACHOQY_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(1555..2016)
                     /locus_tag="ACHOQY_004507"
     CDS             complement(1555..2016)
                     /locus_tag="ACHOQY_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9680462.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(2009..3049)
                     /locus_tag="ACHOQY_004508"
     CDS             complement(2009..3049)
                     /locus_tag="ACHOQY_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9680463.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3021..3572)
                     /locus_tag="ACHOQY_004509"
     CDS             complement(3021..3572)
                     /locus_tag="ACHOQY_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9680464.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3556..3783)
                     /locus_tag="ACHOQY_004510"
     CDS             complement(3556..3783)
                     /locus_tag="ACHOQY_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9680465.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3860..4267
                     /locus_tag="ACHOQY_004511"
     CDS             3860..4267
                     /locus_tag="ACHOQY_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680466.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4532..4831
                     /locus_tag="ACHOQY_004512"
     CDS             4532..4831
                     /locus_tag="ACHOQY_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680467.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4904..5122
                     /gene="ydfC"
                     /locus_tag="ACHOQY_004513"
     CDS             4904..5122
                     /gene="ydfC"
                     /locus_tag="ACHOQY_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9680468.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5145..5552
                     /locus_tag="ACHOQY_004514"
     CDS             5145..5552
                     /locus_tag="ACHOQY_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680469.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENNANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5530..5763)
                     /locus_tag="ACHOQY_004515"
     CDS             complement(5530..5763)
                     /locus_tag="ACHOQY_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680470.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1474 a         1527 c         1274 g         1597 t
ORIGIN      
        1 aaacttatcc gactttacct cgtgcatagc ttgttattta aagtcaacaa aataaggaaa
       61 attatacgca tattgaagag tataaacctt acatgttgat tacatttttg taatcaacat
      121 cctgtttgga atagccagcc tttaatggat aactatttct gacaatgcaa tgagtataat
      181 caagtccatc ttccactgag aattagaggc ggcatgcttt ttccggctct tgccggatat
      241 ccgtaattgt ccataatctg cagatttata ccttctgcat gacctgtcag cgaaaatttg
      301 tccggtgttt ctacgggaat gacatcaaaa gttacacgca ctcgcgttac cgtgtagacc
      361 tactttcctg cacttgcaag atcacagtgg tgtaaccgta acaggaattt attctctgga
      421 ccggcagtaa atccctgagt ggcgtggttc ccatatcaat ttcccgccag gcagcctcca
      481 ttgccagcgt acaggctgga gccatgacct gccctttaaa tctggcccga ccatcccacc
      541 ggacgtgttc ttctcccctg aacttaggta cagtcatctc cagtggcaca aaagtgtcag
      601 cgccataatt tttgaccgtt atcgcgctac ggatattttg ttgactggtg aaaatcaccc
      661 cgcagaatca ggcttattcc cttaacctgg gctttcatcc tgaccgccgc ctcactacga
      721 ccaatcagac tgccgatgca ttttaccttc attgttagta tcatgatttc aggcctgcac
      781 catccgctca ttgcccggac ttccgacaaa tcccggcaac catatcccgg tgcttgttca
      841 gctcccgcag cgcggcgcag actcgctccc acttctggac atgattcttc gccctacgca
      901 gttcgcgatt tgccatatgc agcgatggca aaatcaaatc atcttctcgc gttgcagtaa
      961 acgatggcag cgactgcaca atgtccgcca cagtttctgt tttaatatct tcctgtgttg
     1021 catctttctg taccggtaac gaacaccggc tggctgagga aaggctttac catcggtttc
     1081 cgctaccgat tcaactttcg gctctgctgg taaattatca cctggtatgc agtaacgaat
     1141 tttaccgttc tggtttacgc gaatcagacg acctttgctg attgccattg ccagcgttga
     1201 agcaactttg cgggatgttg taccgaacag cgtagccagt tcatccgccg tttgtggtcc
     1261 gcgttgttca atcgtcgcgg ttaaatcgca ctctgagatt ttcgcttctg ttgccgtggt
     1321 ggtttcttct ggcgctggct gttcctgctg aacgttgtta tcagccacac gccaggtgta
     1381 tacgctttta tcaacgaagc cagccttttt cagttcccac agctcgttca gtacttcttc
     1441 acgactgata tcaagtcgcg cagccagctc taccgacgtg gcttttccca tcgctttcag
     1501 tgcgtcaaaa acagtctcca taaatttcct cccggtaaaa aatcacttct caactcaaac
     1561 aaaaccagcc gctttccggc gttcatattc ctgtttcagc aactcaattg gcgttggccc
     1621 cgacgggcgt ttgggggccg ccagttgtcg ccggactggc ggaacgctca ggccgttact
     1681 aacatgcttt gcccatttcg tcagttgccg ttctgcaagc cgttttaatt ccccttcggt
     1741 catctggcgc tcaatcccct ttgaacgcat ctcgaggcaa atgtgataca gcacaggctg
     1801 agaccacggg tacttatcac ttccgtcgta tcgccaggac tcattgcgcc agcggcggta
     1861 ctcctccatc acagcatcca ccgtcagacc gaatggattg gctccgcttt tcgaaatcag
     1921 tgccacaaac tcagccaggt ccggaggcca tgtttcaccc gcccggcagc ggtccatgca
     1981 ctggcggcag acctgccgga tttgctgctc agtcatcgcg ccaatctgtg caatccagag
     2041 cttcgaaggt gcggccccgt tcttctggat ccagcggttc gaataaacca cccccatgag
     2101 ttcccacagc ttccaggccg tttccgtcgc tgataaatcc gttttcacgt tcccactgct
     2161 cacgtgctgc ccgaatttcc tgaactgccc gtgatgcggt gccacctggt gctgctgcat
     2221 ggtttacccc tttgctgact ggtttaacct gcgccctgac gtgatttacg tgacgggcga
     2281 atttctgctc ccactgaatc tgcgtaaaca ctttcccctc cgctgcccag tagtcccgga
     2341 aggcggcaag ttcagcaggt gtaaattctg tctccggcaa agccatcccc cacaacgcag
     2401 cccgtcgtcg aaaatcccgt gacggatacc agctatcgct catcgggaat tttccgatgg
     2461 gttcgctcag gccatacagg aatacagggg gggctgcctg taacgacaaa acttcctgct
     2521 cactggtcgg agcactctcg cgtgcgttat gtgtggggtt tagatctttg ggttcctttg
     2581 ggttccgtga tccgtttttg ggtgtctttg atggaaaatt tgggtgtctt tggttatttt
     2641 ccatgcagct aagagttctg tttttgggtc tgttttgtgc tgaaacataa ccattttcgg
     2701 tactgttttt attaacagca ccaattttac ccacctttaa agactcccgt ttttgggtgt
     2761 attcaggctc ggcaacactt tcttctacac cgataagtcg gtacaccaca atttgctttg
     2821 ttctgccttt tctctcaccg gtatcaacaa ttaacccaat ctccatcagg tgtcgtaagc
     2881 tgtcctgcac agtctttttg ttcagttccg ttacttctgc cagtgcagat acagacgggt
     2941 atgcacacaa atcggcaccg cacatatcag caagccaggt caatactgac ttactggatg
     3001 aactgccggt tttcaccttt ttagcccatc gtagtgcatc gatactcata cgaacccctg
     3061 gcagacattt gtttatctgc aaagtaatat tggtattgct gacgatacgc gtgcttgaaa
     3121 gcaatagctt tttctataag ctcgtcagtc tcacgttcca caacaactgg atccgcaaaa
     3181 agcagcccgg actccaccac atcgccatat tctttgttta acccggcgat catgtacgta
     3241 atgctttttc cgtcagtaat ttcacaatac aatctgaaat cgctgatccg gatagcctcc
     3301 ataattgccg gaatcagcgc cgtgaatttg tcacgcttat ctctggtgtc gatagctttc
     3361 cagcgttcga atatcttcac ccggttaacg cccagcgccc gttgatcaac ctcgccatca
     3421 ttaaacgtga cgcgttgaac atcgatgttc gggcgttctt tcagagccca gaatgcttcc
     3481 gtgattaata tcgtcgcctg ctcctgtgtc attcctggtc ggcataccca ggtatccaga
     3541 gcctcacaaa cctgttcagt ggtgattttc attgttcaac cgccccgccc gctttgcctt
     3601 acgatattcg tcataaactt tggggtcgta ctgaagttcc ccgccggatg cctcttgcag
     3661 gcgcatcgcg cgaccttcag gaaccagttc cccccatgca gcaacgcttg ccagcctaac
     3721 tcctgcgaca ttggcaagct ttgttttgct gccaaaaaac gctatagcat caattttcaa
     3781 catatcgaac cccttagatt ttcctaagga aactagatcg tagagaaacc taagtcaaga
     3841 aaaattagaa ttacctaata tgaaaaacga aaccttcggt gctcgcctct tatacaggcg
     3901 aaaaaaatta aaactgtctc aggccgcatt aggtaagctg gtcaaagtgg ctcacgtaac
     3961 aatttctcaa tgggaaagag atgaaacaca gccagcgggg aagagattat tcgcactgag
     4021 ccaggcgctt cagtgctcgc cgacttggct tctttttgga gatgaagata agcaaccagg
     4081 cgaaccgatc ccagataatc agccagtcaa tctgacagaa gatcaaaaag agttgcttca
     4141 actgttcgac gcactgcctg agtcagagca aaaggctctg ttgtcagaga tgcgtgctcg
     4201 agttgagaat ttcaacaaac tttttgaaga actactcaaa gctcgcaaaa gaagcgcaaa
     4261 taaataaccc cccttttttt cgcaactctc tgtaataaaa agcacaaact ttcaaatact
     4321 tgtgtttttt acatcaagaa gcttaggttt ttctacacaa aaagcttgac cataactctt
     4381 aggctattct aaattctact catcaagaca ccgcacggtg ttctcagcaa acagttccgc
     4441 tacccggcgt taaggggaag cagaggattt ctcagtgggc gaagtcaaac atcagaatgg
     4501 aaggcgtcca gggatcagca aagaaacagc gatggcgctt tatattgata tcagcgccat
     4561 tgccggacag gtaagagtta tcagagcggt aactaagcgg tatgcgcctt tacttcagaa
     4621 agtctctggt gagtgcaccg aagatattgt caacgatttc gtcatcgaac tgcgaggact
     4681 catcttcagt tacaaggtga ccacaatttt tgcagatggc tcccgcgaaa ctgtcagagc
     4741 cctgcggctt aaaggatgtg tcaaagactt agccaccaca ttctgggcaa gaaaacttga
     4801 ttgtattcat aaccaatttc ctctcgagta acagacccct cagaggatac cacctcgcct
     4861 gacgtggtta aaaagcaggc aacgctaacc acaaggagcc gacatgcaga aacgagaccc
     4921 cgtcatcatc gcgccagact ataccgatga tgaactttat gagtggatgc accagaaaat
     4981 taaggcggtg caggacctga aatgggccaa tgaagccagg gctaagcagg ctgaaaatct
     5041 gtccgctctg gagcaggata tcaccaatct ggaaaaagca gcggcattaa gcattgccag
     5101 aatgattaca tacccacgtt aatagctaac caacgaggct aataatggaa tttaaagatt
     5161 taccaacgcc attacaggaa atggcatcga atatagttcg ttcacaactg gctactcttg
     5221 accttagtac cgcagaaaaa gaaaccatcg ataatatggt tcgtaatgtg cgcaatgctt
     5281 tttctgggct atatggttct gataatcaaa agcaggaaag cgatgttaat aaacgggtaa
     5341 tttctgtttg cgtgaatggc catgttcttt catcaatcaa aacagaaacg gcgacagtct
     5401 tcgattgcct ttgcattgta cagagccttg ttgatgccct gtttcgttca gtgaatttag
     5461 aaaataatgc aaatctgcga gggcgcataa tagcacatcc atatgcacat actttaggct
     5521 ctgtggatat caaagatccc acaaatcttt aatgaaatag ttaacgcgaa ttgtacttgc
     5581 tctttcggtt gctttcagaa tacgcgttga aactgctggc ggtaatttgg tattccattt
     5641 attaaaatca tgcccgggaa agtactcttc gaaaatactt ttaactgcag actcgcctat
     5701 tgaaatgctg cttaccatgc gattttgata aaggcattta gcaataagag ttgattttaa
     5761 cattcaccct cctgagggtt ggtaattaag gagttctcca cgggtgaagt ggagtgcgtg
     5821 cgccggacac gggtgaacat ccggcactga cagtttactg aaaggatatt tc
//
LOCUS       DAZKGS010000228         5851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKGS010000228 DAZKGS010000000
VERSION     DAZKGS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..288
                     /locus_tag="ACHOQY_004516"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHOQY_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHOQY_004517"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHOQY_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP9680471.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHOQY_004518"
     CDS             complement(965..1639)
                     /locus_tag="ACHOQY_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP9680472.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHOQY_004519"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHOQY_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP9680473.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3766)
                     /gene="ureC"
                     /locus_tag="ACHOQY_004520"
     CDS             complement(2114..3766)
                     /gene="ureC"
                     /locus_tag="ACHOQY_004520"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP9680474.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRYIAKYTI
                     NPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDINGSIPT
                     PQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRTVQKADM
                     RHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3792..4112)
                     /locus_tag="ACHOQY_004521"
     CDS             complement(3792..4112)
                     /locus_tag="ACHOQY_004521"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP9680475.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4121..4423)
                     /locus_tag="ACHOQY_004522"
     CDS             complement(4121..4423)
                     /locus_tag="ACHOQY_004522"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP9680476.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4433..5212)
                     /locus_tag="ACHOQY_004523"
     CDS             complement(4433..5212)
                     /locus_tag="ACHOQY_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP9680477.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5593..>5851
                     /locus_tag="ACHOQY_004524"
     CDS             5593..>5851
                     /locus_tag="ACHOQY_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680478.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFP"
BASE COUNT         1312 a         1533 c         1576 g         1430 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgggagaa cgcgccaagg tcgtgcagga
     2761 cgtcttccgc ggcaatggtt tcctggcgaa tgcgcgattc ggcaaaggct acgtcctcgg
     2821 cgatatccgg gtcaagatga tggcaaacca tcagcatgtc cagatgctca tcaatagtgt
     2881 tgacggtata gggtagcgtc ggattggttg aggaaggcag aatattgggg tgcgcgcagg
     2941 cggtgataat atccggagca tgaccaccac ctgcaccttc tgtatggaag gtatggatag
     3001 tgcgcccgcc gatggcagtc agagtatcct caacgaatcc tgattcgtta agcgtatcac
     3061 tgtgtagcgc aacctgaacg tccatttcgt cggctacagt cagtgcgcag ttgattaccg
     3121 caggtgtagc tccccagtct tcgtgaattt tgaggccgat aaccccggcc gcgacctgct
     3181 cacgaagcgc atccggattg gagcaattgc ctttacccag caacccgata ttgaccggca
     3241 ggctgtctgc agcctgcagc atttgataaa tgtaccatgg tcctggggta caggttgtgg
     3301 cgttagaacc cgctgtcggg ccagtaccgc caccgatcat ggtggtaatg ccggatgtca
     3361 gcgcttcttc agcctgctgt gggcagatcc agtgaatatg cgtatcgacg ccacctgcgg
     3421 taacgatcct gccttctgcg gcaataattt ccgtggatac gccgattggg atagtgacgt
     3481 tgggttgtat atcaggatta ccggctttgc cgatagcaaa tatccttcca tctttgacgc
     3541 cgatatcggc tttaacgatc ccccagtaat cgatgatcag ggcattggtc agcaccagat
     3601 cagcgcagcc ggcggagagc atttgcccct gtcccatacc gtcgcggatt actttaccgc
     3661 cgccgaattt gacctcttcg ccgtaggtag ttaaatcatc ttcgacctcg atccacagct
     3721 cagtgtctgc cagacgaatt ttatcaccgg tggtagggcc gaacatgtca gcataggcct
     3781 agcgtgaaat attactcatc atttgcctcc agctcaccca ttatatctcc gcggaaaccg
     3841 tgcacgatac gggcaccgct cagcgccacc agatcaacct cacgcttttg gcctggttcg
     3901 aagcgcactg cagttccggc ggcgatattg agacgatagc ctcttgcctt ctggcgatcg
     3961 aacttcagtg ccgggttaac ctccgcgaag tgataatggg aaccgacctg aattggtcgg
     4021 tctccgtgat tttcaacgat aatactgcag gtagcccggc cgatgttaag ctctatatag
     4081 ccaggtttaa ccttatattc tccagggatc atctgttacc tcagattatc ggattatgga
     4141 cggtaaccag cttggagccg tccggaaagg tggcttcgac ctggatatcg gggatcattt
     4201 ctggtatgcc ttccatgacc tgctcgcgac tcaggacatg ccgtccttct tccatcagcg
     4261 cagcgacgct tttgccgtcg cgagcgccct ccattataaa agcgctaatc agggccacgg
     4321 attcgggata gttaagtttc aggccgcggg ccagacgacg ctctgccagc agtgcagcgg
     4381 taaacagtaa tagtttgtct ttttctcttg gagtcagttc catagcgttc tcttaagtca
     4441 gccagatccg gggaagtacc ggtgatttgt cagtcagttg cggccgcata aattgccaga
     4501 tatcacgcat tacctgctgg caacgcagat tatcatcact taaaaagcga actgtcagta
     4561 gaccatcggt gagcgtggct ccggcgtagt tttccagtgg tgtgagtttt tcgcgtatgt
     4621 cttccagttg cgtttcattc cccaggtaga acagcatcgt tcccacccat ggtttccggg
     4681 caacgcaggt aagttctcca ttggccacgt gcagacgctc aatcaacagc ggagaaccgt
     4741 cgacccatat ctccagacgg ttagcgagtg ttccgtggct gaaggtttct ccgatgaccg
     4801 gacgtccaag gcacagaaga tcccaggcca gcagtgtact ggaggacgac agatgaaaca
     4861 cggtatgcaa cgtggcgtga gcgccgggaa aaaaaatcga atcctgtggc aaccactcca
     4921 gcgttgcgtt attgcacagc gtcaggtttt gctgaaggtg cgcctgcgcg ccgctgctgc
     4981 ggtaaaattt actggcacca ggcatggtga tcagcgtatg gcaatcagag tcaattattg
     5041 cgttgatgat gagttcgtca ccgccgacga taccaccggg tggatgaagc agacagaggt
     5101 ggcaggtttc ttcttcggga taaaaaggac gctgaacagt gagcggaccg acatgatgac
     5161 gggtggtgag cgtggttttc ccgtgcagac gctgaaaacg gagatccagc atggcctgcc
     5221 agcctttttt attttgtggt aacgcggtac tgagcaatct tacgcccctg ataaataaag
     5281 ataaaacgac agtaaagagc cggatttcct tgttatataa aagatgatgc ctttccagtg
     5341 agttttcgtc tgaataatac gaatgtttgt tttcgtcatg aagcgttccg gctcccgggg
     5401 ctatacatcg ccagaaaagc aatgcatatt gtcgggatta tccagagaat caggcagaaa
     5461 gatccgacaa tgggctgtga ttatgccatc tggctcagaa tgatatcaag gtgttatggg
     5521 gataacttgc tgatacaata gcagtatgac agattcatta atgcggaaac tgctcactga
     5581 aacagagtgt tcatgactga gcactggtcg ggtcatgcag atgataattt ccccttttct
     5641 tggggaagcg acagtatttt catgcatgcc tgtcttcagt catacgggga gagcatctgt
     5701 gatacagtcc atacatttgt tgcggacgtc gctcctgagt cactgactgg tctgcaaaca
     5761 gtttatctgt acacggcagg gcttctgtgt gatatcgttt ttcatatcga gggggggcct
     5821 ccagagatga atggaataat aatatttcct g
//
LOCUS       DAZKGS010000229         5833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKGS010000229 DAZKGS010000000
VERSION     DAZKGS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..811
                     /gene="yiaT"
                     /locus_tag="ACHOQY_004525"
     CDS             71..811
                     /gene="yiaT"
                     /locus_tag="ACHOQY_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP9680479.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            complement(758..1021)
                     /locus_tag="ACHOQY_004526"
                     /pseudo
     CDS             complement(758..1021)
                     /locus_tag="ACHOQY_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1025..1126
                     /locus_tag="ACHOQY_004527"
                     /pseudo
     CDS             1025..1126
                     /locus_tag="ACHOQY_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1158..1853)
                     /gene="araD"
                     /locus_tag="ACHOQY_004528"
     CDS             complement(1158..1853)
                     /gene="araD"
                     /locus_tag="ACHOQY_004528"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9680480.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1847..2707)
                     /gene="sgbU"
                     /locus_tag="ACHOQY_004529"
     CDS             complement(1847..2707)
                     /gene="sgbU"
                     /locus_tag="ACHOQY_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9680481.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2700..3362)
                     /gene="ulaD"
                     /locus_tag="ACHOQY_004530"
     CDS             complement(2700..3362)
                     /gene="ulaD"
                     /locus_tag="ACHOQY_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9680482.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3359..4855)
                     /gene="lyxK"
                     /locus_tag="ACHOQY_004531"
     CDS             complement(3359..4855)
                     /gene="lyxK"
                     /locus_tag="ACHOQY_004531"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP9680483.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4859..>5833)
                     /gene="yiaO"
                     /locus_tag="ACHOQY_004532"
     CDS             complement(4859..>5833)
                     /gene="yiaO"
                     /locus_tag="ACHOQY_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP9680484.1"
                     /translation="SVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFN
                     DLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDV
                     PFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLK
                     GLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEV
                     QKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQKIID
                     GMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         1255 a         1596 c         1596 g         1386 t
ORIGIN      
        1 tgcaccggag aaattgtaag cattattatg ccgactcttt tttatcttat tttaaaatgg
       61 aataccggac atgttaatta atcgcaatat tgtggcgtta tttgcgttgc cttttatggc
      121 aagcgcaact gcttctgaat tatccattgg tgctggtgca gcttataatg aatcgcctta
      181 tcgcggttat aatgaaaata caaaggcaat tccgctgatt agttatgaag gtgattcttt
      241 ttatgttcgt cagaccacat taggttttat tctgtcgcaa agtgaaaaaa atgaacttag
      301 cctgaccgca tcctggatgc cgctggaatt tgaccctgcc gataatgacg attatgccat
      361 gcaacagctt gataagcgtg atagtacggc tatggcgggg gttgcctggt atcaccacga
      421 gcgttgggga accgtgaaag cctctgcggc tgcggacgtt ctggataaca gcaacggctg
      481 ggtgggggag ctatcggtat tccacaaaat gcagataggt cgcctgtcgc tgacacctgc
      541 gctgggcgtt ctctattatg acgagaattt cagtgactat tactatggca tttcagagag
      601 tgagtcccgt cgtagcggtc tggcaagtta ttccgcgcag gatgcctggg tgccctatgt
      661 cagcctgacg gcaaaatacc cgataggaga gcacgtcgta ttgatggcga gcgcaggata
      721 cagcgagctg ccggaagaga ttaccaacag cccgatgatt gatcgtaatg agagttttac
      781 ctttatcacc ggcgtgagct ggcgttttta attcaccggt ggatgtcggt gcggctggtg
      841 ctccgtcgta atgccacaaa ggttgttgct ctggattgct ctgttcatac cgggaacctg
      901 gcgtctgaat caggatctta tggctgaaca atcccttgcc ttcaaatgtc gtaaatggcg
      961 catcggcggg cagcaacagg ttatgcagtg actcgacgtt gtcggcctgc tccatgcaat
     1021 acggtttaat gacgttggct atttccggca gatttttcgc aagcacaccg gtttaacgcc
     1081 agcagcgtgg aagcggcgtt acagcaagga acatatcaat tcgtagtgcc ggggcgatga
     1141 agccccggcg tgagggatta ctgcccgtaa taggcattgg ccccatgctt acgcaggtag
     1201 tgcttatcca gcagttcgtt ttgcatcgca gggagctgcg gcgcaagctg gcgcgagaat
     1261 aggcccatat aggcgcattc ttcgagtact acggcgttat gcacggcatc ggcggcgttt
     1321 ttaccccatg cgaacgggcc gtgagaatgc accagcaccg ccgggatttg tgccggactc
     1381 ctgccacgtt cttcgaaggt ttcaatgatc acttcgccgg tctgatattc atattcgccg
     1441 ttaatctcct ctgcggtcat ctgtcgcgtg caggggatgg caccatagaa ataatcagcg
     1501 tgcgtggtgc cccaggcggg gagatccagc ccggcctgcg accagatggt ggcgtggcgt
     1561 gagtgggtat gcacaatgcc accgatttcg gcgtagcgac ggtaaagcgc cagatgcgtt
     1621 ggcgtgtcgg acgagggctt tttgctacct tccaccacct tgccgctggc tatctcaacc
     1681 accaccatat cgtcggcggt catcacgtca tactcgacgc cggaaggttt gattaccatc
     1741 cattgccgcg tttcgtctac cgcgctgaca ttgccccagg tgaacgtcac cagatggtga
     1801 gcgggaagcg ccagattcgc cgccagcacg tcggctttca gttgctctaa catataaatc
     1861 cagcctcctg catacgcgct tcaatccaac gccgcgcctg aataatctcc agcaccggct
     1921 ctttggcttt ttcggtccac atctcaatca ggaaagaacc gcgatagttc agtttatgca
     1981 gcgttttgaa gatgccaacg aaatcgacgc agccttcgcc aaacggcacg tcgcggaact
     2041 gtccgggact ttgctcggta actggctggg tgtctttcag gtggatcgcg gcgatacggt
     2101 caatgcccag tttcagttcg gcgggaacat cattgcccca ggcgctgagg ttgccgacgt
     2161 ccgggtagac ggtgaaccac ggcgaggcga gcatctcgtc ccactttttc catttactga
     2221 tggagttcat aaacgcggtg tccataatct ccaccgctag catgacttgt gatgccgccg
     2281 cctgttcgac cgcccacgcc agcccttcag caaaacgttg ccgggtgcct tcatcgtggt
     2341 cttcgtaata gacgtcgtag cccgccagct ggatggtgcg tatgccgaga tcgcgtgcca
     2401 gacgaatcgc tttgctcatg atctcccgtg ctcgctggcg taccgcgtcg tcacggctac
     2461 caaaaggaaa gcgacgatgt gcggacaggc acatcgacgg gatcccaaca cccgtttcga
     2521 tcatcgcggc aaccagcgaa gtcctttgtg cggtgctcca gtcaagacgt gaaagccgtt
     2581 cgtcggtttc atccaccgac atttcgacaa aatcaaaacc gcagcttttt gccagcacca
     2641 gccgctccgg ccaggagaga tctttcgcca gcgccttttc ataaatccct aacggatgat
     2701 tacgcacgct tgcctcccca gatagcgtcg atttgcgcat ggaaatcgcc agccacctgc
     2761 gccggatttg ctgagcctgc cagtgctcgc ccggcaataa acgctttcac gcgaatatct
     2821 ttaaacagcg gcaggtcagc aggggtaatc ccgccggtaa tggaaagctc aaggccgata
     2881 tcggaaagcg ccttcatgcg tgccagatcg gcttcgcccc actgttgccc gctggcctgt
     2941 gcatcacgac cgcgatgata aatcgcctgc cgcacgccaa tacgatgcca gtcgcgggcg
     3001 tcgtccagcg tccagttacc gaacagctcg atctgaattt caccaccgca gcgttgcgcc
     3061 atcgcgtggc ctttttccac cgtcgcgagc ggtgcggcgc agatgatggt catccagtta
     3121 gcgcctgcgc caaatgcctg ttgcgcgagc gtttcaccag cgtcggcgac cttccagtcg
     3181 gcaacgatga ttttgtcagg gcactgttcg cgcaaggctt tcaccgcgcc aagcccttcg
     3241 tttaaacaga gaatggtgcc cgcttcgaca atatcgacgc tgtcttttaa ctgcgtcacg
     3301 tcgcgctgtg cggcttcaag tgatgagtgg tcgagtgcca gttgcagaag tggtcggctc
     3361 ataatatgtg ctccttgatg cgggcgtgaa agccctgaag tgcggcaatg agatgctgat
     3421 agcgttggta tttttgttgg taaagctgat ggacagtcat atccggcagc agggtgcgca
     3481 ccgggtgctg taaatcacgc tgggcttcgc tgaaatcgcg ataaaccccg gtgccgacgc
     3541 gggcggcaag tgctgcacca aagcagccgg tttcttccac ctgcggcagc tcaatacgca
     3601 gaccgctgac gtccgccagc atttgcatcc agacatcgga atgcgccggg ccgccagtga
     3661 cgcgcagggt gtgcacatca gtaaaacgtt cgcgcatccg gttgagatgg gtcatatggc
     3721 tgaacaccac cccttcataa atggcctgca acaggtgtgc gcgggtgtga atggcctgca
     3781 tcccgtagaa accgctggtc atctcgagtc cggcgttgct gccgtacagg aacggcagga
     3841 aaaagagatc gccaccggcc ttcggcaagc tggcaacggc ctggttgatc tcatcaaacg
     3901 agatttctcc ccactgtgcg gtaaaccatt cgaggttgcc ggaagaggtg gggctggctt
     3961 cgtgaacgat aaattcacca tcgttaacgt agcgaccata gacatacgga tgcgcttcac
     4021 cgtcacgtaa accgcgggtt atgccgctgg tcaccgccca ggtgcccatc accgcattaa
     4081 gggtaaattc gtcttcgatc ccggcgcaga gtgcggtgga aaccacatca aacaggccgc
     4141 cgacaacggg cgtacccgct ttcagaccgg tcagtacggc tgtctgagcg gtgatctccc
     4201 cgcagatttc ggcagatccg acaacaggcg gcagggtgtg attgatttca gcaatcccca
     4261 gccagtcggt gaggcacggg tcatattccc caagactcat gttgtagaga ttggactcgg
     4321 aaatattgct ctcttcacag cctttgacgc cggttaaaca ccagcgcagg tagtcgtgcg
     4381 tcatcatcac gcagccaatt tgcgcgtagc gttccggttc gtgctcttta agccaacgta
     4441 acagcgatac cggatgcccg gtccacaagg tttgtcgggt cagcgggtag agtttttccg
     4501 ggatgccatc ttcctgccag cgacgaacga tttccatcgc ccggcgatcc gaggataaaa
     4561 tggcgttacc gagcggcttg ttgtttttat ccagcaaaaa caagcctttt ccttgtgcgg
     4621 agataccgat accgacaatt tgttccccgc taacgccgga atgggtaagc agttcgcgaa
     4681 tgacagccat gcagcattgc cacagttctg ccatatcgcg ctctgcccaa cctggctgcg
     4741 ggcttaatgc gcacagcggc aggcgctgca cgcctgcctc ccggccttcg cggtcataca
     4801 gcccggcttt cagccagcta ccgccacaat ctaaccccag ccagtattgc gtcatggttt
     4861 attgcacctc atccacggct ttcagtagat cagcaccctg cggaacatct tttacaaaca
     4921 tgtcgcggac ctgagtaccc agtgcgtcgc taaaggcttt gcggtcgaga tcggtgatga
     4981 cttccacgcc cgcttctttc atgccgtcga tgattttttg ctgatcttcg gcaaccagtt
     5041 tgcgctggta gttacccgct tcctgcgcag atgaaattag cgcttgctgg aactccgggc
     5101 ttaagccatc aaacttcgct ttgttgatca ccaccagaag cggggaatag gcgtggtgcg
     5161 tcagagaaag gtacttctgc acttcgaaaa attttgctga ccagacgacg ttgatcgggt
     5221 gttcctgagc gtcgatagtg cgggtttcca gcccggtata gacttcggca aacggcatcg
     5281 ggatcgggtt agcgccaaag actttgaatg cggcgatatt catcgggcta ttgttggtgc
     5341 gaattttcag ccctttcagg tcggcggggg ttttaaccgg tgcgcgcgag ttggtgacat
     5401 cgcgccagcc gttttcccag taggccagta cttttagtcc tttaccttca agtgaggctt
     5461 tcagatcatc accgactttg ccgtcgagcg ttttatgcgc gtgagcggta tcgcggaaca
     5521 ggaaagggac atcaagcaag ttcatcactg gtgataatcc ggcaaagtta ttcgagccgg
     5581 acatttccat atcgatggtg ccgccacgta cgccgctgat catcgcctgc gcgttaccga
     5641 gggtgctatc cggaaacaat tttaatttca gctcgccttt ggttctctcc tgcaataaat
     5701 cattgaattt tttcgccgca atatgttgcg agtcggtttg tgaggtttca taaccgaaac
     5761 gtaaagattg cgccgccaga gaagatgtgc tgaatgcagc caggccagca atgaataatg
     5821 cgtaggttac aga
//
LOCUS       DAZKGS010000230         5824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKGS010000230 DAZKGS010000000
VERSION     DAZKGS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            295..1059
                     /gene="pdhR"
                     /locus_tag="ACHOQY_004533"
     CDS             295..1059
                     /gene="pdhR"
                     /locus_tag="ACHOQY_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9680485.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            1220..3883
                     /gene="aceE"
                     /locus_tag="ACHOQY_004534"
     CDS             1220..3883
                     /gene="aceE"
                     /locus_tag="ACHOQY_004534"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9680486.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            3898..5790
                     /gene="aceF"
                     /locus_tag="ACHOQY_004535"
     CDS             3898..5790
                     /gene="aceF"
                     /locus_tag="ACHOQY_004535"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9680487.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
BASE COUNT         1493 a         1468 c         1591 g         1272 t
ORIGIN      
        1 atttaaattc gttttaattg aattaaaaat cacaaaattg gtaagtgaat cggttcgatt
       61 cggattttta tagtttaata atcgttaaaa aactcctttc ctacgtaaag tctacatttg
      121 tgcatagtta caactttgaa acgttatata tgtcaagttg ttaaaatgtg cacagtttca
      181 tgatttcaat caaaacctgt atggacataa ggtgaatact ttgttacttt agcgtcacag
      241 acatgaaatt ggtaagacca attgacttcg gcaagtggct taagacagga actcatggcc
      301 tacagcaaaa tccgccaacc aaaactctcc gatgtgattg agcagcaact ggagtttttg
      361 atcctcgaag gcactctccg cccgggcgaa aaactcccac cggaacgcga actggcaaaa
      421 cagtttgacg tctcccgtcc ctccttgcgt gaggcgattc aacgtctcga agcgaagggc
      481 ttgttgcttc gtcgccaggg tggcggcact tttgtccaga gcagcctatg gcaaagcttc
      541 agcgatccgc tggtggagct gctctccgac catcctgagt cacagtatga cttgctcgaa
      601 acacgacacg ccctggaagg tatcgccgct tattacgccg cgctgcgtag taccgatgaa
      661 gacaaggaac gcatccgtga actccaccac gccatagagc tggcgcagca gtctggcgat
      721 ctggacgcgg aatcaaacgc cgtactccag tatcagattg ctgtcactga agcggcccac
      781 aatgtggttc tgcttcatct gctaaggtgt atggagccga tgttggccca gaatgtccgc
      841 cagaacttcg aattgctcta ttcgcgtcgc gagatgctgc cgctggtgag tagtcaccgc
      901 acccgcatat ttgaagcgat tatggccggt aagccggaag aagcgcgcga agcatcgcat
      961 cgccatctgg cctttatcga agaaattttg ctcgacagaa gtcgtgaaga gagccgccgt
     1021 gagcgttctc tgcgtcgtct ggagcaacga aagaattagt gatttttctg gtaaaaatta
     1081 tccagaagat gttgtaaatc aagcgcatat aaaagcgcgg caactaaacg tagaacctgt
     1141 cttattgagc tttccggcga gagttcaatg ggacaggttc cagaaaactc aacgttatta
     1201 gatagataag gaataaccca tgtcagaacg tttcccaaat gacgtggatc cgatcgaaac
     1261 tcgcgactgg ctccaggcga tcgaatcggt catccgtgaa gaaggtgttg agcgtgctca
     1321 gtatctgatc gaccaactgc ttgctgaagc ccgcaaaggc ggtgtaaacg tagccgcagg
     1381 cacaggtatc agcaactaca tcaacaccat ccccgttgaa gaacaaccgg agtatccggg
     1441 taatctggaa ctggaacgcc gtattcgttc agctatccgc tggaacgcca tcatgacggt
     1501 gctgcgtgcg tcgaaaaaag acctcgaact gggcggccat atggcgtcct tccagtcttc
     1561 cgcaaccatt tatgatgtgt gctttaacca cttcttccgt gcacgcaacg agcaggatgg
     1621 cggcgacctg gtttacttcc agggccacat ctccccgggc gtgtacgctc gtgctttcct
     1681 agaaggtcgt ctgactcagg agcagctgga taacttccgt caggaagttc acggcaatgg
     1741 cctctcttcc tatccgcacc cgaaactgat gccggaattc tggcagttcc cgaccgtatc
     1801 tatgggtctg gggccgattg gtgctattta ccaggctaaa ttcctgaaat atctggaaca
     1861 ccgtggcctg aaagatacct ctaaacagac cgtttacgcg ttcctcggcg acggtgaaat
     1921 ggacgaaccg gaatccaaag gtgcgatcac catcgctacc cgtgaaaaac tggataacct
     1981 agtcttcgtt atcaactgta acctgcagcg tcttgacggc ccggtcaccg gtaacggcaa
     2041 gatcatcaac gaactggaag gcatcttcga aggtgctggc tggaacgtga tcaaagtgat
     2101 gtggggtagc cgttgggatg aactgctgcg taaagatacc agcggtaaac tgatccagct
     2161 gatgaacgaa accgttgacg gcgactacca gaccttcaaa tcgaaagatg gtgcgtacgt
     2221 tcgtgaacac ttcttcggta aatatcctga aaccgcagca ctggttgcag actggactga
     2281 cgagcagatc tgggcactga accgtggtgg tcacgatccg aagaaaatct acgctgcatt
     2341 caagaaagcg caggaaacca aaggcaaagc gacagtaatc cttgctcata ccattaaagg
     2401 ttacggcatg ggcgacgcgg ctgaaggtaa aaacatcgcg caccaggtta agaaaatgaa
     2461 catggacggc gtgcgtcaca tccgcgaccg tttcaatgtg ccggtgtctg atgcagatat
     2521 cgaaaaactg ccgtacatca ccttcccgga aggttctgaa gagcatacct atctgcacgc
     2581 tcagcgtcag aaactgcacg gttatctgcc aagccgtcag ccgaacttca ccgagaagct
     2641 tgagctgccg agcctgcaag acttcggcgc gctgctggaa gagcagagca aagagatctc
     2701 taccactatc gctttcgttc gtgccctgaa cgtaatgctg aagaacaagt cgatcaaaga
     2761 tcgtctggta ccgatcatcg ccgacgaagc gcgtactttc ggtatggaag gtctgttccg
     2821 tcagattggt atttacagcc cgaacggtca gcagtacacc ccgcaggacc gcgagcaggt
     2881 tgcttactat aaagaagacg agaaaggtca gattctgcag gaagggatca acgagctggg
     2941 cgcaggttgt tcctggctgg cagcggcgac ctcttacagc accaacaatc tgccgatgat
     3001 cccgttctac atctattact cgatgttcgg cttccagcgt attggcgatc tgtgctgggc
     3061 ggctggcgac cagcaagcgc gtggcttcct gatcggcggt acttccggtc gtaccaccct
     3121 gaacggcgaa ggtctgcagc acgaagatgg tcacagccac attcagtcgc tgactattcc
     3181 gaactgtatc tcttacgacc cggcttacgc ttacgaagtt gctgtcatta tgcatgacgg
     3241 tctggagcgt atgtacggtg aaaaacaaga gaacgtttac tactacatca ccacgctgaa
     3301 cgaaaactac cacatgccgg caatgccgga aggtgctgag gaaggtatcc gtaaaggtat
     3361 ctacaaactc gaaaccattg aaggtagcaa aggtaaagtt cagctgctgg gctccggttc
     3421 tatcctgcgt cacgtccgtg aagcggcaga gatcctggcg aaagattacg gcgtaggttc
     3481 tgacgtttat agcgtgacct ccttcaccga actggcgcga gatggtcagg attgtgaacg
     3541 ctggaacatg ctgcacccgc tggaaactcc gcgcgttccg tatatcgctc aggtgatgaa
     3601 cgacgctccg gcagtggcat ctaccgacta tatgaaactg ttcgctgagc aggtccgtac
     3661 ttacgtaccg gctgacgact accgcgtact gggtactgat ggcttcggtc gttccgacag
     3721 ccgtgagaac ctgcgtcacc acttcgaagt tgatgcttcc tacgtggttg tagcggcgct
     3781 gggcgaactg gctaaacgtg gcgaaatcga taagaaagtg gttgctgacg caatcgccaa
     3841 attcaacatc gatgcagata aagttaaccc gcgtctggcg taagaggtaa aagaataatg
     3901 gctatcgaaa tcaaagtacc ggacatcggg gctgatgaag ttgaaatcac cgagatcctg
     3961 gtcaaagtgg gcgacaaagt tgaagccgaa cagtcgctga tcaccgtaga aggcgacaaa
     4021 gcctctatgg aagttccgtc tccgcaggcg ggtatcgtta aagagatcaa agtctctgtt
     4081 ggcgataaaa cccagaccgg cgcactgatt atgattttcg attccgccga cggtgcagca
     4141 gacgctgcac ctgctcaggc agaagagaag aaagaagcag ctccggcagc agcaccagcg
     4201 gctgcagcgg caaaagacgt taacgttccg gatatcggca gcgacgaagt tgaagtgacc
     4261 gaaatcctgg tgaaagttgg cgataaagtt gaagctgaac agtcgctgat caccgtagaa
     4321 ggcgataaag cgtctatgga agttccggct ccgtttgctg gcaccgtgaa agagatcaaa
     4381 gtgaacgtgg gtgacaaagt gtctaccggc tcgctgatta tggtcttcga agtcgcgggt
     4441 gaagcaggcg cggcagctcc ggcggctaaa caggaagcgg ctccggcagc ggcccctgca
     4501 ccagcggctg gcgtgaaaga agttaacgtt ccggatatcg gcggtgacga agttgaagtg
     4561 accgaagtga tggtgaaagt gggcgacaaa gttgccgctg aacagtcact gattaccgta
     4621 gaaggcgaca aagcttctat ggaagttccg gctccgtttg caggcgtcgt gaaggaactg
     4681 aaagtcaacg ttggcgataa agtgaaaact ggctcgctga ttatgatctt cgaagttgaa
     4741 ggcgcagcgc ctgcggcggc tcctgcgaaa caggaagcgg cagcgccggc tccggcagca
     4801 aaagctgaag ctccggcagc agcaccggct gcgaaagcgg aaggcaaatc tgaatttgca
     4861 gaaaacgacg cttacgttca cgcgactccg ctgatccgcc gtctggcacg cgagtttggc
     4921 gttaacctgg cgaaagtgaa gggcactggc cgtaaaggtc gtatcctgcg cgaagacgtt
     4981 caggcttacg tgaaagaagc tatcaaacgt gcagaagcag ctccggcggc gactggcggc
     5041 ggtatcccag gcatgctgcc gtggccgaag gtggacttca gcaagtttgg tgaaatcgaa
     5101 gaagtggaac tgggccgtat ccagaaaatt tctggtgcga acctgagccg taactgggtg
     5161 atgatcccgc atgttactca cttcgacaaa accgatatca ccgagctgga agcgttccgt
     5221 aaacagcaga acgaagaagc ggcgaaacgt aagctggatg tgaagatcac cccggttgtc
     5281 ttcatcatga aagccgttgc tgcagctctt gagcagatgc ctcgcttcaa cagttcgttg
     5341 tcggaagacg gtcagcgtct gaccctgaag aaatacatca acatcggtgt ggcggtggat
     5401 accccgaacg gtctggttgt tccggtattc aaagacgtca acaagaaagg catcatcgag
     5461 ctgtctcgcg agctgatgac tatttctaag aaagcgcgtg acggtaagct gactgcgggc
     5521 gaaatgcagg gcggttgctt caccatctcc agcatcggcg gcctgggtac tacccacttc
     5581 gcgccgattg tgaacgcgcc ggaagtggct atcctcggcg tttccaagtc cgcgatggag
     5641 ccggtgtgga atggtaaaga gttcgtgccg cgtctgatgc tgccgatttc tctctccttc
     5701 gaccaccgcg tgatcgacgg tgctgatggt gcccgtttca ttaccatcat taacaacacg
     5761 ctgtctgaca ttcgccgtct ggtgatgtaa gtaaaagagc cggcccaacg gccggctttt
     5821 ttct
//
LOCUS       DAZKGS010000231         5768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKGS010000231 DAZKGS010000000
VERSION     DAZKGS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(229..705)
                     /locus_tag="ACHOQY_004536"
     CDS             complement(229..705)
                     /locus_tag="ACHOQY_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP9680488.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(764..2053)
                     /gene="bioA"
                     /locus_tag="ACHOQY_004537"
     CDS             complement(764..2053)
                     /gene="bioA"
                     /locus_tag="ACHOQY_004537"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9680489.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2140..3180
                     /gene="bioB"
                     /locus_tag="ACHOQY_004538"
     CDS             2140..3180
                     /gene="bioB"
                     /locus_tag="ACHOQY_004538"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9680490.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3177..4331
                     /gene="bioF"
                     /locus_tag="ACHOQY_004539"
     CDS             3177..4331
                     /gene="bioF"
                     /locus_tag="ACHOQY_004539"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9680491.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4318..5073
                     /gene="bioC"
                     /locus_tag="ACHOQY_004540"
     CDS             4318..5073
                     /gene="bioC"
                     /locus_tag="ACHOQY_004540"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9680492.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5066..5743
                     /gene="bioD"
                     /locus_tag="ACHOQY_004541"
     CDS             5066..5743
                     /gene="bioD"
                     /locus_tag="ACHOQY_004541"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9680493.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1345 a         1528 c         1620 g         1275 t
ORIGIN      
        1 ctatctaagt agttgattca tagttactag atatgttgtg ttttgtagta ttatgcagtc
       61 tattttttag actaaatata ttgtaacaca ttgatattaa tggtctttag cgtttcgcgt
      121 tcagcttttt tatactaact tgagcgaaac gggaaggtaa aaagacaaaa agttgttttt
      181 aataccttta agtgatacca gatggcattg cgccatctgg cagagtgatt aactaaacat
      241 cgcagtaatc gaggcgcttg ccagagagtg gaaatgaacg ttaaacccga ccatcgcgcc
      301 gctggcacct tcatcgacat caatacgttc tatatccagc gcgtgaacgg taaaaatgta
      361 gcgatgagtt tcgcctttcg gcggtgctgc gccatcgtac ccggttttac caaagtcggt
      421 acgcgtctgc aaaacgccgt ctggcattgc taccagacca gagccaaacc cttgcggtaa
      481 tacgcgggta tcagcgggta agttaacaac tacccagtgc caccagccgg agccggttgg
      541 cgcatccggg tcgtagcagg tgacaacaaa acttttcgtt cccgcaggaa catcatccca
      601 cgccagatgc ggtgaaatat tatcgccatc gtaacccatg ccgttaaaga catgacgatg
      661 cggcaattta tcgccatcgc gcagatcgtt actgatgagt ttcatgaacc ctcctttctt
      721 gtttgcagaa agtgtagcca gaaaccctca cgcggacttc tcgttattgg caaaaaaatg
      781 tttcatcctg taccgcgcgg ttaaccgctg cggtcagacg ctgcaactgt tgcgggagaa
      841 taatataggg cggcatcagg taaatcagtt tgccaaaagg ccggatccag acaccctgtt
      901 cgacaaagaa tttttgcagc gccgccatat tcaccggatg agtggtttcg actacgccga
      961 ttgcccccag cacgcgcaca tcggcaacca tttcggcatc acgggcgggg gcaagttgct
     1021 cgcgcagctg tacttcaata gccgccacct gttgctgcca gtcgccagat tcgagaatcg
     1081 ccaggctggc gtttgctgcc gcgcaggcca gcggattgcc cataaaagtt ggcccatgca
     1141 taaagcaacc ggcttcgccg ttactgatgg tttccgcaac ctcgcgcgtg gtgagcgtgg
     1201 cggaaagggt cattgtgccg ccggttaagg ctttaccgag gcacaaaatg tccggcgcga
     1261 tttctgcatg ttcacaggca aacagtttgc cggtacgacc aaatccggtg gcgatctcgt
     1321 cggcaatcag caagatacct tcgcgatcgc acattttgcg gattcgtttt aaccattccg
     1381 gatggtacat gcgcatcccg cctgcgccct ggacaatcgg ttcaatgatc accgccgcga
     1441 tttcatgacg atgcgccgcc atcaggcggg caaagccaac catatcacgt tcatcccatt
     1501 caccatccat gcggctttgc ggggcgggag caaacaagtt ttctggcaga tagcctttcc
     1561 acaggctgtg cattgagttg tccggatcgc acaccgacat cgcgccaaag gtatcgccat
     1621 gataaccatt gcggaaggtc agaaaacgct ggcgcgcttc gcctttggcc tgccagtact
     1681 gcaacgccat tttcatcgcc acttccaccg ctacggagcc ggagtccgcg agaaaaacgc
     1741 attccagcgg ttgcggcgtc attgccacca gtttgcggca tagctcgatg gcgggcgcat
     1801 gggtgatacc gccaaacatc acatgcgaca tagcatcaat ttgcgacttc atcgccgcat
     1861 taagctgcgg gtgattatag ccgtggatcg ccgcccacca ggacgacata ccgtcaacca
     1921 ggcgtctgcc gtcagacaaa attagctcgc aaccttcggc gctcgcgacc ggataaaccg
     1981 gcagagggga ggtcatggat gtgtatgggt gccagatatg gcgttggtca aaggcaagat
     2041 cgtccgttgt cataatcgac ttgtaaacca aattgaaaag atttaggttt acaattctac
     2101 accgaattaa caacaaaaaa cacgttttgg agaagcccca tggctcaccg cccacgctgg
     2161 acattgtcgc aagtcacaga attatttgaa aaaccgttgc tggatctgct gtttgaagcg
     2221 cagcaggtgc atcgacagca tttcgatcct cgtcaggtgc aggtcagcac tttgctgtcg
     2281 attaagaccg gagcttgtcc ggaagattgc aaatactgcc cgcaaagctc gcgctacaaa
     2341 accgggctgg aagccgagcg gttgatggaa gttgaacagg tgctggagtc ggcgcgcaaa
     2401 gcgaaagctg cgggatcgac gcgtttctgt atgggcgcgg cgtggaagaa tccccacgaa
     2461 cgcgatatgc cgtacctgga acaaatggtg cagggggtaa aagcgatggg gctggaggcg
     2521 tgtatgacgc tgggcacgtt gagtgaatct caggcgcagc gccttgcgaa tgccgggctg
     2581 gattactaca accacaacct cgacacctcg ccggagtttt acggcaatat catcaccacc
     2641 cgcacttatc aggaacgcct cgatacgctg gaaaaagtgc gcgatgccgg gatcaaagtc
     2701 tgttctggcg gcattgtggg cttaggcgaa acggtaaaag atcgcgccgg attattgctg
     2761 caactggcaa acctgccgac gccgccggaa agcgtaccaa tcaacatgct ggtgaaggtg
     2821 aaaggcacgc cgctggcgga taacgatgat gtcgatgcct ttgattttat tcgcaccatt
     2881 gcggtcgcgc ggatcatgat gccgacctct tacgtgcgcc tttctgccgg acgcgagcag
     2941 atgaacgaac agactcaggc gatgtgcttt atggcaggcg caaactcgat tttctacggt
     3001 tgcaaactgc tgaccacgcc gaatccggaa gaagataaag acctgcaact gttccgcaaa
     3061 ctggggctaa atccgcagca aactgccgtg ctggcagggg ataacgaaca acagcaacgt
     3121 cttgaacagg cgctgatgac cccggacacc gacgaatatt acaacgcggc agcattatga
     3181 gctggcagga gaaaatcaac gcggcgctcg atgcgcggcg tgctgccgat gccctgcgtc
     3241 gccgttatcc agtggcgcaa ggagccggac gctggctggt ggcggatgat cgccagtatc
     3301 tgaacttttc cagtaacgat tatttaggtt taagccatca tccgcaaatt atccgtgcct
     3361 ggcagcaggg tgcggagcaa tttggcgtcg gtagcggcgg ttccggtcac gtcagcggtt
     3421 atagcgttgc gcatcaggcg ctggaagaag aactggccga gtggctgggc tattcgcggg
     3481 cactgctatt tatctctggt tttgccgcta atcaggcggt cattgccgcg atgatggcaa
     3541 aagaggaccg cattgtcgct gaccggctta gccatgcctc attgctggag gctgccagtt
     3601 taagcccgtc gcagcttcgc cgttttgttc ataacgatgt cactcatctg gcgcgactgc
     3661 ttgcttcccc ctgtccgggg cagcaactgg tggtgacaga aggcgtgttc agcatggacg
     3721 gcgatattgc gccactggcg gaaatccagc aggtaacgca acagcacaat ggctggttga
     3781 tggtcgatga tgcccacggc acgggcgtta tcggggagca ggggcgcggc agctgctggc
     3841 tgcaaaaggt aaaaccagaa ttgctggtgg tgacttttgg caaaggattt ggcgtcagtg
     3901 gggctgcggt gctttgctcc agtacggtgg cggattatct gctgcaattc gcccgccacc
     3961 ttatctacag caccagtatg ccgcccgctc aggcgcaggc attacgtgcg tcactggcgg
     4021 tcattcgccg tgatgagggg gatgcacggc gcgaaaaact ggtgtcactc attgcgcgtt
     4081 ttcgtgccgg agtacaggat ttgccgttta cgctggctga ttcatgcagc gccatccagc
     4141 cattgatcgt cggtgataac agccgtgcgt tacaactggc agaaaaactg cgccagcaag
     4201 gctgctgggt cacggcgatt cgcccgccaa ccgtacccgc tggtactgcg cgactgcgct
     4261 taacgctaac cgcagcgcat gaaatgcagg atatcgaccg tctgctggag gtgctgcatg
     4321 gcaacggtta ataaacaagc cattgcagcg gcatttggtc gggcagccgc actctatgag
     4381 caacacgcag atctacagcg ccagagtgct gacgtcttac tggcaatgct cccacagcgt
     4441 aaatacaccc acgtactgga cgcgggttgt ggacctggct ggatgagccg ccactggcgg
     4501 gaacgtcacg cgcaggtgac ggccttagat ctctcgccgc caatgcttgt tcaggcacgc
     4561 cagaaggatg ccgcagacca ttatctggcg ggagatatcg aatccctgcc gttagcgact
     4621 gcgacgttcg atcttgcatg gagcaatctc gcagtgcagt ggtgcggtaa tttatccacg
     4681 gcactccgcg agctgtatcg ggtggtgcgc cccggaggcg tggtggcgtt taccacgctg
     4741 gtgcagggat cgttacccga actgcatcag gcgtggcagg cggtggacga gcgtccgcat
     4801 gctaatcgct ttttaccgcc agatgaaatc gaacagtcgc tgaacggcgt gcattatcaa
     4861 catcatattc agcccatcac actatggttt gatgatgcgc tcagtgccat gcgttcgctg
     4921 aaaggcatcg gtgctacgca tcttcatgaa gggcgcgacc cgcgaatatt aacgcgttcg
     4981 caattgcagc gattgcaact ggcctggccg caacaacagg ggcgatatcc tctgacgtat
     5041 catctttttt tgggagtgat tgctcgtgag taaacgttat tttgtcaccg gaacggatac
     5101 cgaagtgggg aaaaccgtcg ccagttgtgc acttttacaa gccgcgaagg cagtaggtta
     5161 tcggacggca ggttataaac cggtcgcctc tggcagcgaa aagaccccgg aaggcttacg
     5221 taatagtgac gcgctggcgt tacagcacaa cagcagcctg cagctggatt acgcaacggt
     5281 aaatccttac accttcgcag aacccacctc gccgcacatc atcagcgcgc aagagggcag
     5341 gccgatagaa tcattggtaa tgagcgccgg attacgcgcg cttgaacaac aggctgactg
     5401 ggtgttagtg gaaggtgctg gcggctggtt tacgccgctt tctgacactt tcacttttgc
     5461 agattgggta acacaggaac aactgccggt gatactggta gttggtgtga aactcggctg
     5521 tattaatcac gcgatgttga ctgcgcaggc aatacaacac gccggactga ctctggcggg
     5581 ttgggtggcg aacgatgtta cgcctccggg aaaacgtcac gctgaatata tgaccacgct
     5641 cacccgcatg attcccgcgc cgctgctggg agagatcccc tggcttgcag aaaatccaga
     5701 aaatgcggca accggaaagt acataaacct tgccttgttg tagctatcct gtatttggtt
     5761 aaattgcg
//
LOCUS       DAZKGS010000232         5763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKGS010000232 DAZKGS010000000
VERSION     DAZKGS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..521
                     /locus_tag="ACHOQY_004542"
     CDS             174..521
                     /locus_tag="ACHOQY_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680494.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(726..1427)
                     /locus_tag="ACHOQY_004543"
     CDS             complement(726..1427)
                     /locus_tag="ACHOQY_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9680495.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1424..2443)
                     /locus_tag="ACHOQY_004544"
     CDS             complement(1424..2443)
                     /locus_tag="ACHOQY_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9680496.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2440..2979)
                     /locus_tag="ACHOQY_004545"
     CDS             complement(2440..2979)
                     /locus_tag="ACHOQY_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP9680497.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(3049..3279)
                     /locus_tag="ACHOQY_004546"
     CDS             complement(3049..3279)
                     /locus_tag="ACHOQY_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9680498.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3384..4073
                     /locus_tag="ACHOQY_004547"
     CDS             3384..4073
                     /locus_tag="ACHOQY_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9680499.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4155..4418
                     /locus_tag="ACHOQY_004548"
     CDS             4155..4418
                     /locus_tag="ACHOQY_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680500.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            4431..4874
                     /locus_tag="ACHOQY_004549"
     CDS             4431..4874
                     /locus_tag="ACHOQY_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680501.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            5425..5631
                     /locus_tag="ACHOQY_004550"
     CDS             5425..5631
                     /locus_tag="ACHOQY_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP9680502.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
     gene            5707..>5763
                     /locus_tag="ACHOQY_004551"
     CDS             5707..>5763
                     /locus_tag="ACHOQY_004551"
                     /inference="COORDINATES: protein motif:HMM:NF017841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9680503.1"
                     /translation="MNAYYIQDRLEAQSWARHY"
BASE COUNT         1530 a         1372 c         1273 g         1588 t
ORIGIN      
        1 acgactcatt cttatgactg gaaaaagcaa tcatgctaat aatcattgtt gttccagttc
       61 caaatgcagc gaaataatca ccaatatttt ttctaccggt ttcgactttg atgctatatt
      121 ctgctaacac ctaactaaca ctacgtttca cacagcaatt acatggaaat aacatgacta
      181 tcaaagcgac tgacatagag attcttcatc gttatgctga aggggtaatg gaacgttcta
      241 accatcatgc aaaaaatgtt ggtgcagccg ctcttacatt gctaggaggt gttatctgga
      301 aagccctgcc cggatcaatc gagataagaa cgtataacgg aagtcttgca aatatggtgt
      361 ggtggcaaag cgaaagaaca ttaaaaaatt atgcaatctc atacaaccat aattcatgtg
      421 aaattgaaat gagagatgaa tcagtcaaag gtgctgtatt atttagcatt tccaacgaaa
      481 ccactccaga aaaaatatta tcacagcttt ctgagcttta attaaaggcg gtgctaacac
      541 cgcccatacc attactccac atcttacgca acttgataat tatgctctat taaataccca
      601 caatatttca tcaatgcatg ggtcagctac aaaatgttta ccgtggtaat tcctgttttc
      661 gcaacatctc aaaagaacaa taaacactgt atttcttcaa gtaacgaaga atctttcttt
      721 gcccgtcata cacttgctcc tttcagccca aacttagctt tgatttctgc gatcttcgcc
      781 agagcctgtg cacgatttag aggtctaccg cccataacag gaagttgttt tactggttca
      841 ggtatcgtct caccacggtt aattcgcgct gtcatacagg tcagttcatc ggcagccttg
      901 cgccgtaatt ccgcgtcagt cagcgcattg gcccgcatgt tctggtacag gttggtaacc
      961 aaccagtaat gcgcgttcga tttccacgga taagactctg catccggata caggccacgc
     1021 ttccggcaat actcgtaaac catatcaacc agctcgctga cgtttggcag cccggcgtta
     1081 acggatgctt cttcccggca ccatgcaaca aactgcccgg gtgatggcag aaatggtcta
     1141 ttctgccgac gggcaacacg cattccggcg ctcacctgtt ccatcgtggt gatcccgttt
     1201 tcccggaaag ccagaaccca ctggcggcgg atttcgttca gttcgttctg gtcacgatta
     1261 gccaggctcg ccgggaaagt tgccagtaac tggctgaata caccgttgat tatctgcgct
     1321 acctgctgta cctgcggctt ttcgtcgtac tgttccggca tgttgttggc gatccgacgc
     1381 atctgctcac ggtcaaagtt aatcatctgt gcggcgatgt ttttcataga tccaccccgt
     1441 aaatccagtc tgtgtttgtc aggtcgagtt ttggtttgcc gactgtcacg ccagcctgtt
     1501 gcttgttccg gttgatttcg agctgggtcc acttgtcgcg gagtttggcc ggactcagca
     1561 cgttaccgga ccagaagttg tcctggcagg cccagcggaa cagtacacac atgtcgcggt
     1621 ggttacgtcc atcacgttca cgcatcagac ggatatcgtt agcccaccct gcaaaattcg
     1681 gttttctggc tgatggcgcg atggtcttca ccatgtcaaa catccactct gcggcggtca
     1741 ggtcttctgc tgtcccccac ttgctgccgc tctgaatcgc agcatccgct ttcaccacag
     1801 gaaggtcgtt ttctggcagg tcagaggatt cgccagaatt ctcggacgaa taaggtttta
     1861 tattgtcttt tgttagtttg tcttttgtgt ttacctgatt cgggtaagtg cctttacctg
     1921 atttgggtaa acttttctta cctgattcag gtaaatttac ctctttcagg taaactttat
     1981 ttttcttacc tgattcgggt aatgttgacc attcactgac cacattatta atgccgatat
     2041 tccgcccgct ctgaataaaa atcccacgct ttaccagaac actttttgca gcagaacact
     2101 tgtgcggcaa tatcccggtc aactcggaaa gttgctcgtt gctcacccag tccagttttt
     2161 tattaaagcc atatgttttg cgcatgacag ccaggaagac cagaagctgg tgctgtgtta
     2221 atccggccag cattacagct tccagcaact catttgcaat gcgcgtataa ccatcatcga
     2281 gatctgccac gcgcggctcc ttttgtgccg catccggcac tggaaaattg aatatctcag
     2341 cagtgtttgc cataattcct cccgcaatga gtgtgttacg atttgcacct gaaagtcggt
     2401 tctgttccag cagaccggct ttcgccattt ctgaacctgt catatcgccc ccagcatggt
     2461 agtaaccatc gccatcaatg gaccagccag ctctgggtcc acacgaaaca tcgacacaat
     2521 accttcacta atttccttca gtttctggtg gcgtggtgcg ttgagaatga cagcctgttt
     2581 tgcctcactg agttcctttt ccatttcagc caacctagcc atgaagctat cctgctcaac
     2641 caggtaaccg cgatattcca gcggtagtac cgccagaatt gccggggtca gttcacgcac
     2701 gttatttcgg tatttttcag aatcgaattt gttatcgagg aagcggaaca gcttctggcg
     2761 tgtacggctg acatcatcag ggaaatcgat ggtgccgccg ccctgctccc gatactcatt
     2821 cacaatgagt gtggcaacga catcctgatt atctacagcc gaccaggcgc ggacggcatc
     2881 acggattttt tcgtggcctg gcacctgttt tgtttgagaa cgatttatca ccgcagtcgg
     2941 gctaaatccg ctagtctgtt ggtatggaag tggttgcata attgactcct ttagtttgaa
     3001 ttgactgtta agttgattgc ttattgttaa agagcgtgaa atggaaattt aagctgcgtt
     3061 cttttcggtg tgtggaaaca acttcggaag atccgggcga atctggtatg ccttcactac
     3121 tccaccagta gccgtaacaa tgctgccgac atgttcaggg gatacctttg ctttgttgtg
     3181 aagccactta tagacggcct gctgtgaaac ttcgcaggca gcgcccagtt tcttttgtga
     3241 accaacgata ttgatcgctg ttttgatagc tgggttcata acaacctccg tggttaattt
     3301 gaatcaagat taaaactatg gttgttttta gtcaacaacc attttcgttt gatggaataa
     3361 aaccttggtt gtacatttgg actatgaaaa caacactctc agaaagactt aaagaagcca
     3421 gattagcgcg aggccttaca caaaaggcgc ttggggattt ggtcggggtt agccaggctg
     3481 ctattcagaa aatcgaaaca gggaaagcta atcaaacaac taaaatcgtg gagatcgcga
     3541 acgctttggg tgtgcgcgca gaatggttat cttctggcgt tggaaatatg tcagacagta
     3601 cagtgcaacc aatacaatca actgtcagcc attccaaata cttcaagatt gacgttcttg
     3661 atatagaagt cagtgctggg ccgggagtca tcaaccgtga gtttgtagaa gttctacgct
     3721 cggttgagta ctcgtttgac gatgctcgtc acatgttcga tggtaggaag gcagaaaata
     3781 tccgcatcat taacgtgcgt ggtgacagca tgtcaggaac gatcgaacca ggtgatctgc
     3841 tgttcgttga tatcacggtt aaatctttcg acggtgatgg tatctatgca tttctgtacg
     3901 acgacaccgc ccatgtaaag cgcctgcaaa tgatgaagga taaactgctg gttatctctg
     3961 ataacaagag ctactcaccg tgggacccta tcgagaaaga cgagatgaac cgggtattca
     4021 tattcggtaa ggttattggg agcatgccgc agacgtacag aaaacacgga taatcagccg
     4081 cgtgttgatg aggcttttgg gtaatacgct gaagaaaata ccttataaaa aataacatta
     4141 cgggaaaggc aaaaatgagt aataaaacac ttgttaaaaa atcaaataac tgtgtcgatg
     4201 catacgccga cgcgtttggc tactcttcgt ttggtgacgg ctctcagcgc ctaggttcaa
     4261 tttctttttt tcataatgta actgaatggc ctgttgatga aagtgacgat gttgagaaca
     4321 taaagtacaa cattgcaacc attagaatgc ccgaagaatt gatgcttaaa ttggctgact
     4381 tcattcgcga ccagcatgac aaagctaaaa caaactaatc gtaattaatt atgaaacacg
     4441 attattacga agctccaaac ctaaatgata aaattgcaaa ggcggcagaa tcattttgtt
     4501 tgcactccag tgcgtcttcc cagctagcag gtatatctgg aaaaagatgg gacatgctac
     4561 aatcaagtgg tagcagtggg tctgggagtg ataacatgcc tgaaaaagtt gcaaaactgg
     4621 agtccgatgt tgctcatatc aaacgagacg ttgatgaact taaaacagac gttaaagcta
     4681 tcgacaaaaa tatgataact attcttgcac gtctggactc tataaaagaa tccttaacaa
     4741 acaagccatc aagtgatgct gtcgatagaa agatttcaga cgcaaagctt gcggtattgc
     4801 ttggtgttcc agcaatcatc gctgcaggaa caggacttta taagctatca atgtactttt
     4861 tctttagtgc ttaagaaaat gctcgcaccc ttgcattagg taaagcgatt tttcattccc
     4921 cattaccctc gctcttaacc aatcatctaa acccggccac cgtgccgggt tttcttttgc
     4981 cctcccctca tcatacaccg ttcaaaaaaa ccaccacgac ctcgcttcag ttatcgctat
     5041 gcgatgcaag tcacaaaata aatccatctt aaatacaacc agttatatct aaaacaacca
     5101 acaaaacaac ttttgttgtt gacgataaaa caactatagt tttaaatgag ttcatcgcaa
     5161 caacacaacg atacggcaat cacctgattc accgttgcga tgaccgctta gatccgcagc
     5221 ttgaatttca gcaggcttcg gggagtgcga gggatgaaac ggacgcgtga acgtcggtgt
     5281 gaccagctga aatcaactca acatttcata ccttagtcgc ttcaacgagg cggcttagtt
     5341 atgacaaccg gcggccatcc accgcctgaa tacgcgcaga agtctttata tgttcagcag
     5401 cccagcttac gggcaggagt ttttatggtt catcaacatt acggaacgca gaccgttaat
     5461 cgcggtgcgg tcatgccagg aatgctggtc aaacgcaaag atggtacctg gactgcatca
     5521 gctaatttac gcggacgact ttatctgcat cgcggcattg agcgcactta tacccgtgac
     5581 ttgctcgtgg aagtttttct cgacggacgc ggcaacgctc tgaatcacta atcccctttc
     5641 ctgttttccg aatcagcctg gcattccgcg ggcgattttt tcacagccat tttcaggagt
     5701 tcagccatga acgcttatta cattcaggat cgtcttgagg ctcagagctg ggcgcgtcac
     5761 tac
//
LOCUS       DAZKGS010000233         5761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKGS010000233 DAZKGS010000000
VERSION     DAZKGS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..352
                     /locus_tag="ACHOQY_004552"
     CDS             <1..352
                     /locus_tag="ACHOQY_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9680504.1"
                     /translation="GTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPL
                     SAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQ
                     MELVMKAAGFKEVK"
     gene            complement(392..862)
                     /gene="yehS"
                     /locus_tag="ACHOQY_004553"
     CDS             complement(392..862)
                     /gene="yehS"
                     /locus_tag="ACHOQY_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9680505.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(909..1628)
                     /gene="btsR"
                     /locus_tag="ACHOQY_004554"
                     /gene_synonym="yehT"
     CDS             complement(909..1628)
                     /gene="btsR"
                     /locus_tag="ACHOQY_004554"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9680506.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1625..3310)
                     /gene="btsS"
                     /locus_tag="ACHOQY_004555"
     CDS             complement(1625..3310)
                     /gene="btsS"
                     /locus_tag="ACHOQY_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9680507.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3532..3597
                     /locus_tag="ACHOQY_004556"
                     /pseudo
     CDS             3532..3597
                     /locus_tag="ACHOQY_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3825..4073
                     /locus_tag="ACHOQY_004557"
     CDS             3825..4073
                     /locus_tag="ACHOQY_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9680508.1"
                     /translation="MRVESCIAKEKISKMPKGSVPALQQEMLRRIRKRYDNAEIIIKS
                     ASNNGLTILRATDKESAHEFVQETLQEARETADDWFIH"
     gene            4695..5486
                     /locus_tag="ACHOQY_004558"
     CDS             4695..5486
                     /locus_tag="ACHOQY_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avirulence protein"
                     /protein_id="HFP9680509.1"
                     /translation="MGHLERGLWKTETRWDGNDREQLGKEALGSEEPKPGSPFYGSRG
                     LKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQTGQVLAGRLKIQERVKFRADNRI
                     DAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYAAEHFGK
                     TWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRYDAGMEEKYVNTLDI
                     FTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT         1650 a         1405 c         1289 g         1417 t
ORIGIN      
        1 cggcactgaa attgccatta cctatgtcta caaaggtgac aaggtgctta agcaatcttc
       61 tgaaaccaaa attcaatttg cttctattgg tgcaaccacc aaagaagacg ctgccaagac
      121 acttgagccg ttaagcgcca aatacaaaaa catcgcgggt gttgaagaaa aattaaccta
      181 tacagatacc tacgcgcagg aaaacgtgac tatcgatatg gaaaaagtgg attttaaagc
      241 cctgcagggt atttcaggaa tcaacgtttc tgctgaagat gccaaaaaag gtatcactat
      301 ggcgcaaatg gaactggtga tgaaagccgc tggttttaaa gaagtgaaat aatcggttgg
      361 cggtcatgct ctaaacatga ccgccaattt tttagccttt tttcacatgc tggcgcgctg
      421 ccagtccacg cagaaaataa cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat
      481 ccggtgcgcg catcatcgct gtaatttccg gcatcgaaac gcggaactgc tgttcggtga
      541 ggatagccag aatgtcatcg gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga
      601 tgttgttatt aatgcgacgt tccggctcca gtgccggagc agactcatcc ttgccgcgtt
      661 tttcataaat caggccattg aggaatgacg acaaaaaaat gtccggacaa cgctgaaaac
      721 cctcttcgtc ttctttacgt agccagacgg cgatctgttc cgcggtggct tcgacattac
      781 ccagcgccag aatacgcacc aggtcattat tattggcttt caaaatgtag cgcacgctgc
      841 gcagaatatc gttacttagc atgaggcctt caggtgttga tgaggcaaaa agccatttta
      901 gcagtctttt acaggccaat cgcctctttt aagcttttca gataacggcg gctgaccggc
      961 acggttaagc cattacgcaa aatcaactcg gcctggccgt tatcttccag acgaatctcc
     1021 tgtaaatgcg cgaggttaac cagatactga cgatggcagc gcagtagtgg tgtacgactt
     1081 tccagggtac gtaatgtcaa ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag
     1141 acaccgctca tccgactgct gacaaatgcc acatctttca tttgcagcaa ataaatccga
     1201 ctatgccccg tacaagggat aaatttcagc gcctgttgat tttccggtaa cagcgaaaca
     1261 tcctgcttgc tgcgctcctg acgcaatcgc gccagcgttt tctccagtcg cgcttcatca
     1321 attggcttca gcagataatc aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca
     1381 aacgcagtga gaaaaacaat atacgggcga tgttccgggt caagcatccc caccatttcc
     1441 agaccactga tgcgcggcat ctggatatcg agaaacagca catccgggcg cagtttatgc
     1501 accgcgccga tcccttccac ggcgtttgaa cactctccaa cgatttcaat atcgctctgc
     1561 tcctgcaaaa atacgcgcag gttctcccgt gctaacggtt catcatcgac aattaagact
     1621 ttaatcatgc ctcgtccctc catggtagtc gtaacgttat tcgggtgtaa ctatcaggct
     1681 cacaggcgac gcttattcca tagtcatcgc caaaccgttc acgtaaacgc ttatccacca
     1741 gattcatccc cagcccactg gcattggtta ccggttgata caaaccggca ttgtcttcga
     1801 tctccagcat caaatgttgc ccctcacgtc gggcgctgat tgccactcgc cctgtatcca
     1861 gcagttgtga tgtcccatgt ttaatggcgt tttccactat cggttgcagg gtaaacgcgg
     1921 gcaattgctg ctgggataat tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga
     1981 agcgcgcctt ttcaatttgc agataagcgt tcacatgttc aatttcgtcg gcgagagtaa
     2041 caaactccga aggccgcttt aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct
     2101 ggctggcctg ttcgctgtcg cggcggatca ccgctttaat ggtgttaagc gcattaaaca
     2161 aaaaatgagg attcacctgg gcgtgaagca gtttgatttc tgactgggtg agcatcgctt
     2221 tttgccgctc atattgcccg gcaagaatct gcgccgaaag cagttgcgca atcccctcgc
     2281 ccagcgtgcg gtttattgaa ctgaataaac ggtttttggc ttcatacaat ttgatggtgc
     2341 ccatcacgcg ctgattttcg ccacgcaacg gaattaccag cgtcgacccc agtttgcatt
     2401 gcggatgcaa agagcaacga taaggtactt cgttgccatc agcgtagacc acttcaccgg
     2461 tttcaatcgc ttttaaggtg taagtcgaag aaatcggttt gccgggtaaa tggtggtcgt
     2521 caccaattcc ggtaaaggcc agcaatttct ctcgatcggt aatcgcgact gcaccaatat
     2581 ccagctcctg atacagcacc tgagccactt tcatgctgtt cacttcgtta aacccctgtc
     2641 gcaaaatgcc ttccgtcgag gctgccactt tcagcgcagt ggcagaaaaa gcagaagtgt
     2701 atttttcaaa catcgcgcgt ttatcgagca atatacgcat aaacagcgcc gcgccgacgg
     2761 tattggtgac catcattggt gcagcaatat tactcaccag acgcaccgca tcttcataag
     2821 gtcgggcgat cgcaaggatg atcagcattt gcaccatttc agcgacgaac gtgacggcac
     2881 cggcggtaat ggggttaaag actttatcag tgcgcccgcg acggatcagg atgctgtgta
     2941 ccaggccgcc gagcaatcct tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc
     3001 cccccatcga atatcgatgt aagccgccgg tcagaccaac cagcccaccg acgaccggac
     3061 cgccgagtaa gccgcccatt accgcgccta tcgcacgggt attggcaata gaatcgtcaa
     3121 tgtgcaaccc aaaccaggtg cccatgatgc agaagatgga aaagacgatg tagcagagaa
     3181 atttatgcgg cagacgaacc gtgacctgca ttaacggtat gaataatggc gttttactca
     3241 ttaaccacgc aatgactaaa aaaacgcaca tctgctgaag cagcagcaac accagattaa
     3301 aatcgtacat acccgcaaac cacacttccc tttaaaacgc gtaacataca ttgcctgcgt
     3361 ttaactttct ttgaactctt gcagaaaaat gagaattcgt gagtacgatc actcaaaatc
     3421 gcctggcaaa aataaaatca ccctatagat gcacaaaaaa cgggcaaaac tacctggttc
     3481 gcaaaactgc gtctaaagtt aaaccgggac ctcgcgagca agggtgagac gatggcgctt
     3541 tacacaattg gtgaagtggc gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga
     3601 cgcaacattt ttaaaaatca acgaagatcg tccaggagcg ccgattttca agggttggac
     3661 agataattcg ttttcgatgt cgacgaactt cgacggattt cgcctataaa aagtgtccca
     3721 tacatgcccc attgatgccc catagacaat atcagcaatt gacactggtt atctatacaa
     3781 ctaaaaataa cacctgtaca taaccacagt tacaggaggg tgttatgcgt gttgagagct
     3841 gtatcgctaa agaaaaaatc agcaaaatgc caaaaggttc tgttcctgcc ctgcaacagg
     3901 aaatgctgcg ccgtatcagg aaacgatatg acaatgcaga aatcatcatc aaatctgcca
     3961 gtaataatgg actcactatt ttaagagcca cagataaaga gtccgcacac gaatttgtac
     4021 aggaaacact tcaggaagcc agggaaacag cggacgactg gtttattcac tgactaaaat
     4081 cccgtccaca tgccgggaat ttcctatgtc cgaagaaaat ggtgtggtta actaagcaaa
     4141 agtgtccctt tatcggtata gcaactcatt ttgttaacat acggttgcaa aattgatgac
     4201 aagtgtttat tttagccaca agaaatgtat gcaaaccaga attgggaaat gcatttataa
     4261 aataaggaat agcagatatg tttgcagcaa agcccgaatt aagtcaaaga tgtatttctg
     4321 gactcctcac aactgggcca aatgatgaaa acgtaaataa taaaccgtca agtgatatgc
     4381 cgaacagaat caaactagtt gtaagcgatt cacaaatcgg tatatataca actgacagta
     4441 atactctgaa ggatggattg tgtagccaag cccacatagg aactcaacct tatcggccag
     4501 gtgctgactc gccgataact gtgagatcat tcctacaaat cacactttcc aacaataatt
     4561 acaaatcatt agagcatctg caaacatgag cagagaatta tctgaggtat cgcaaagcag
     4621 aagagaatat tctgcgttcg accgttgagg ggctaaccaa tccagaatct cctgtattca
     4681 aacaaacagc atggatgggg catctggagc gtggcctttg gaaaacggaa acacgctggg
     4741 atggaaacga ccgcgagcag ctaggcaaag aggctcttgg ctcagaggag ccaaaacctg
     4801 gttcaccatt ttacggtagt cgtggactaa aattatccga cagtgcgcat tctgccttct
     4861 caatgatgct gtgcggttct gaaggtccat tcacaaaaga acaagcactc tctggtttcg
     4921 agcttgctca aactggccaa gtgctggctg gaaggctaaa gattcaggag cgagttaaat
     4981 tccgcgcaga caaccgcatt gatgctcagc gtaacggtac acactcaacc cgcacaccaa
     5041 caggcatgga tctttctcaa gatatcggca caattatgcg tgataaagct gggctaccgg
     5101 tcatgagcgg aacatccggt tcctcctctg atgccactct tgcaacacgt tacgctgccg
     5161 agcattttgg taagacatgg gccgctccag gcttaagcca agcagaagga tgcaaagcta
     5221 tctcggatct gtcacatcac tattttcgtg cagaaggctc cagtccacca caatcaatgg
     5281 caacgggaat aaataaggtt cgttatgatg caggaatgga agagaaatat gtaaatacac
     5341 tggatatatt tacacatagc tacccagaaa tttatgctgg ggttgcattg acgattgctg
     5401 gggctggcgg taatgatgag caggcaatgt ataatgttac gcaagaagcc gctcgtattc
     5461 tgcacgaagc tgaaactaaa gattgaacat tctcaataaa gagctgcgcc aggcagcaca
     5521 tattcagcat tgcagtaaga tatgcatgcc cagccccctg taaacctata aatatttcaa
     5581 atcatattga ttatcttata gccatatttt ttatgacacc ctgtttcagt gaacatgaag
     5641 acactcacct tgttcctgat attcattagc atcatcctga ttatttaact tctctattca
     5701 gggaagcccg tctgatacaa agcttctatt cctcgttaaa tattctccat tctggcgcca
     5761 a
//
LOCUS       DAZKGS010000234         5694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKGS010000234 DAZKGS010000000
VERSION     DAZKGS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..270
                     /gene="mazE"
                     /locus_tag="ACHOQY_004559"
     CDS             22..270
                     /gene="mazE"
                     /locus_tag="ACHOQY_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP9680510.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            270..605
                     /gene="mazF"
                     /locus_tag="ACHOQY_004560"
     CDS             270..605
                     /gene="mazF"
                     /locus_tag="ACHOQY_004560"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP9680511.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            676..1467
                     /gene="mazG"
                     /locus_tag="ACHOQY_004561"
     CDS             676..1467
                     /gene="mazG"
                     /locus_tag="ACHOQY_004561"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9680512.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            1695..3332
                     /gene="pyrG"
                     /locus_tag="ACHOQY_004562"
     CDS             1695..3332
                     /gene="pyrG"
                     /locus_tag="ACHOQY_004562"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9680513.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            3420..4718
                     /gene="eno"
                     /locus_tag="ACHOQY_004563"
     CDS             3420..4718
                     /gene="eno"
                     /locus_tag="ACHOQY_004563"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9680514.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4778..5650)
                     /gene="ygcG"
                     /locus_tag="ACHOQY_004564"
     CDS             complement(4778..5650)
                     /gene="ygcG"
                     /locus_tag="ACHOQY_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9680515.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
BASE COUNT         1543 a         1298 c         1457 g         1396 t
ORIGIN      
        1 tagcggtttg aggaaagggt tatgatccac agtagcgtaa agcgttgggg aaattcaccg
       61 gcggtgcgga tcccggctac gttaatgcag gcgctcaatc tgaatattga tgatgaagtg
      121 aagattgacc tggtggatgg caaattaatt attgagccag tgcgtaaaga gcccgtattt
      181 acgcttgctg aactggtcaa cgacatcacg ccggaaaacc tccacgagaa tatcgactgg
      241 ggagagccga aagataagga agtctggtaa tggtaagccg atacgtaccc gatatgggcg
      301 atctgatttg ggttgatttt gacccgacaa aaggtagcga gcaagctgga catcgtccag
      361 ctgttgtcct gagtcctttc atgtacaaca acaaaacagg tatgtgtctg tgtgttcctt
      421 atacaacgca atcaaaagga tatccgttcg aagttgtttt atccggtcag gaacgtgatg
      481 gcgtagcgtt agctgatcag gtaaaaagta tcgcctggcg ggcaagagga gcaacgaaga
      541 aaggaacagt tgccccagag gaattacaac tcattaaagc caaaattaac gtactgattg
      601 ggtagtgtta ctaactcact tctattctgg tcacaggttt ccacctgaca gacccgcaat
      661 tttcaggaca attcaatgaa tcaaatcgac cgtttgctca ctattatgca gcgcctgcgc
      721 gatccggaaa acggctgccc gtgggataaa gagcagacat ttgccaccat tgcgccttac
      781 acccttgaag aaacctacga agtgctggac gccatcgccc gtgaagattt tgacgatctt
      841 cgcggtgaac tgggcgatct gctattccag gtggtgtttt acgcgcaaat ggctcaggaa
      901 gaagggcgct ttgactttaa tgatatttgc gctgctatta gcgataaatt agagcgtcgc
      961 catccgcatg tttttgctga tagttctgcc gaaaacagta gtgaagtgct tgcccgttgg
     1021 gagcaaatca aaaccgaaga gcgcgcgcag aaagcgcagc attcggcgct ggacgatatt
     1081 cctcgtagtt taccggcttt aatgcgtgcg caaaaaatcc agaaacgttg cgccaacgtt
     1141 ggcttcgatt ggacgacgct tggtccggta gtcgataaag tctacgaaga gatcgacgag
     1201 gtgatgtacg aagcgcggca ggctgttgtc gaccaggcta aactggagga ggaaatgggg
     1261 gacctgctgt ttgccacggt caatctggct cgtcatttag ggacaaaagc ggaaatcgca
     1321 ttgcaaaaag cgaacgaaaa atttgagcgt cgttttcgcg aagtggagcg tattgttgcc
     1381 gcgcgtggac tggaaatgac aggtgttgac ctcgaaacaa tggaagaagt ctggcaacag
     1441 gtaaaacggc aggaaattga tctctaaggg aattacgcgg tcaagcgcca tttgtgtcat
     1501 tttttaaatg acaagcgctt gatttgcgtc aaaaacattt accccaaagg ggctattttc
     1561 tcactcctga tttcaatagt gcgctggcga agaggaggga taatgaaagt ttgtggcaca
     1621 ggtcatgttc gggtatactg ctttcccgtc ctggttattc catcgtcttt tcaacctaac
     1681 ttctcaggtt cagcatgaca acgaactata tttttgtgac cggcggggtc gtatcctctc
     1741 tgggtaaagg cattgccgca gcctccctcg cagccattct tgaagcccgt ggcctcaatg
     1801 tgaccatcat gaaactggat ccgtacatca acgtcgatcc aggtactatg agcccaatcc
     1861 aacacgggga agtgttcgtt actgaagacg gcgctgaaac cgacctggac ctggggcact
     1921 acgagcgttt cattcgtacc aaaatgagcc gccgcaacaa cttcaccacg ggtcgtatct
     1981 actctgacgt tctgcgtaaa gaacgccgcg gtgactacct cggcgcaacc gtgcaggtta
     2041 ttccgcacat cactaacgca atcaaagagc gcgtgctgga aggtggcgaa ggtcatgacg
     2101 tagtactggt agaaatcggc ggtacagtag gtgatatcga atccttgccg ttcctcgaag
     2161 cgattcgcca gatggctgtt gaaattggcc gtgagcacac tctgtttatg cacctgacgc
     2221 tggtgccgta catggcagcg tctggtgaag tcaaaaccaa accgactcag cactctgtaa
     2281 aagagctgct ctccatcggt atccagcctg acatcctgat ttgtcgttca gatcgcgctg
     2341 ttccggcgaa cgaacgtgcg aagattgcat tgttctgtaa tgttccggaa aaagcggtta
     2401 tttctctgaa agacgtcgat tccatctata aaattccggg cctgttgaaa tctcaggggc
     2461 tggacgatta tatttgtaaa cgattcagct taaactgccc ggaagcgaat ctgtccgaat
     2521 gggaacaggt tatcttcgaa gaagcgaacc cggtaagtga agtcaccatc ggtatggtcg
     2581 gcaagtacat tgaactgccg gatgcttata aatcagtgat cgaagcactg aaacacggtg
     2641 ggctgaagaa tcgtgtcagc gtcaacatca aactgatcga ttcacaagat gttgaaacgc
     2701 gcggcgttga aatccttaaa ggtctggacg caatcctcgt acctggcggt ttcggctatc
     2761 gtggcgtaga aggcatgatt acgaccgcgc gttttgcgcg tgagaacaat attccttatc
     2821 tgggcatttg cctgggtatg caggtggcgt taattgatta cgctcgccat gttgccaaca
     2881 tggagaacgc caactctacg gaatttgtgc cagactgtaa gtacccggtt gtggcgctga
     2941 ttaccgagtg gcgcgatgaa aacggcaacg ttgaagttcg tagcgagaag agcgatctcg
     3001 gcggtaccat gcgtctcggc gcacagcagt gccagttggt tgacgatagc ctggttcgcc
     3061 agctgtacaa tgcgccgaca attgttgagc gtcatcgtca ccgttacgaa gtcaacaaca
     3121 tgctgttgaa acagattgaa gatgcaggtc tgcgcgttgc gggccgttcc ggggatgatc
     3181 agttggtcga gatcatcgaa gttccgaatc acccgtggtt cgtggcttgc cagttccatc
     3241 cggagtttac ttctactcca cgtgatggtc acccgctgtt tgcaggcttt gtgaaagccg
     3301 ccagcgagtt ccagaaacgt caggcgaagt aagtaaaaaa gttagaacgg caacgcgtac
     3361 cctgggtacg cgttgtttgt ctggagtttc agtttaacta gtgacttgag gaaaacctaa
     3421 tgtccaaaat cgtaaaaatc atcggtcgtg aaatcatcga ctcccgtggt aacccgactg
     3481 ttgaagccga agtacatctg gagggtggtt tcgtcggtat ggcagctgct ccgtcaggtg
     3541 cttctactgg ttcccgtgaa gctctggaac tgcgcgatgg cgacaaatcc cgtttcctgg
     3601 gtaaaggcgt aaccaaagct gttgctgcgg taaacggccc gatcgctcag gcgctgattg
     3661 gcaaagatgc taaagatcag gctggcattg acaagatcat gatcgacctg gacggcaccg
     3721 aaaacaaatc caaattcggc gcgaacgcaa tcctggctgt atctctggct aacgccaaag
     3781 ctgctgcagc tgctaaaggt atgccgctgt acgagcacat cgctgaactg aacggtactc
     3841 cgggcaaata ctctatgccg gttccgatga tgaacatcat caacggtggt gagcacgctg
     3901 acaacaacgt tgatatccag gaattcatga ttcagccggt tggcgcgaaa actgtgaaag
     3961 aagccatccg catgggttct gaagttttcc atcacctggc aaaagttctg aaagcgaaag
     4021 gcatgaacac tgcagttggt gacgaaggtg gctatgcgcc gaacctgggt tccaacgctg
     4081 aagctctggc tgttatcgct gaagctgtta aagctgctgg ttatgaactg ggcaaagaca
     4141 tcactctggc gatggactgc gcagcttctg aattctacaa agacggtaaa tacgttctgg
     4201 ctggcgaagg caacaaagcg ttcacctctg aagaattcac tcacttcctg gaagaactga
     4261 ccaaacagta cccgatcgtt tctatcgaag acggtctgga cgaatctgac tgggacggtt
     4321 tcgcatacca gaccaaagtt ctgggcgaca aaatccagct ggttggtgac gacctgttcg
     4381 taaccaacac caagatcctg aaagaaggta tcgaaaaagg tatcgctaac tccatcctga
     4441 tcaaattcaa ccagatcggt tctctgaccg aaactctggc tgcaatcaag atggcgaaag
     4501 atgctggcta cactgcagtt atctctcacc gttctggcga aactgaagac gctaccatcg
     4561 ctgacctggc tgttggtact gctgcaggcc agatcaaaac tggttctatg agccgttctg
     4621 accgtgttgc taaatacaac cagctgattc gtatcgaaga agctctgggc gaaaaagcac
     4681 cgtacaacgg tcgtaaagag atcaaaggcc aggcataaga ctgactttat ctgatttaaa
     4741 aatgccagcc tccgggctgg cattttttat ggttgagtta ccagcggccg gatgcgccgc
     4801 cacctccaga agaaccgcca ccgccgctaa atccgccgcc acttgagcct ccaccggaac
     4861 cgctgttgtc tgaatgataa cttttacctg atgctctttt ctgaagtaca cataaacagg
     4921 taaagaccat aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaaattt
     4981 tatcggagaa caggaataaa tctaaaagat agattgctga aatatagact ccacttttac
     5041 atgcgcgaca aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa
     5101 atacccaaaa aatagcaaag taataatgat cactggaaga cgctgattca ctttctgaag
     5161 gattagtcgg atattggtgt tgtgaagtga gttggttatt caaagcgttt atagctagct
     5221 ctaatccctg agcaaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat
     5281 ctccagccag agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag
     5341 tacgatctga ccaggcaaca atgatcagta tcccatcatt acgtttggca tctcctaaac
     5401 gccaattgtc aaaaactctt gtagcatatt gttcaattgt ctcgtctttg gttgtgggaa
     5461 caattaatac agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca
     5521 gttctttctt ttcttgtgag tttaagctac tcgtaagatc cgtaacttgt tgctgtaatt
     5581 gtgggacaat agttggttgg gctgcaacaa aggaacacac gaaagtgaac attagtatga
     5641 aatagcgcat tataaatttc tcattaaatc tggataaata aattgacggc ggca
//
LOCUS       DAZKGS010000235         5554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKGS010000235 DAZKGS010000000
VERSION     DAZKGS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..285)
                     /locus_tag="ACHOQY_004565"
     CDS             complement(<1..285)
                     /locus_tag="ACHOQY_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680516.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHD"
     gene            809..1783
                     /gene="hemB"
                     /locus_tag="ACHOQY_004566"
     CDS             809..1783
                     /gene="hemB"
                     /locus_tag="ACHOQY_004566"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9680517.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPSVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1889..2740)
                     /gene="tauD"
                     /locus_tag="ACHOQY_004567"
     CDS             complement(1889..2740)
                     /gene="tauD"
                     /locus_tag="ACHOQY_004567"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9680518.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2737..3564)
                     /gene="tauC"
                     /locus_tag="ACHOQY_004568"
     CDS             complement(2737..3564)
                     /gene="tauC"
                     /locus_tag="ACHOQY_004568"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9680519.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3561..4328)
                     /gene="tauB"
                     /locus_tag="ACHOQY_004569"
     CDS             complement(3561..4328)
                     /gene="tauB"
                     /locus_tag="ACHOQY_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9680520.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4341..5303)
                     /gene="tauA"
                     /locus_tag="ACHOQY_004570"
     CDS             complement(4341..5303)
                     /gene="tauA"
                     /locus_tag="ACHOQY_004570"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9680521.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1366 a         1529 c         1358 g         1301 t
ORIGIN      
        1 gtcatgattc aggtaatatg tagagatagt cccgttaaca gtgtcaccat taaaggtggt
       61 atcgaaatca ccgtttgtgt tgttatagta gacaaagcca ctataattca catagttacc
      121 atctacagta cccggtgcat ccatattgct ccagtcaaga gcggtagttg gattgtcgta
      181 ataagtccag gtgtcataag tagttgcatt agcctgagag gtgatagcca gagtgcaagc
      241 caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc atccctcctc
      301 agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt atgtttcgcg
      361 ctaatgatta tgctgcgtta cagacgaatg gttcaattat tctttcccct aagtccgaat
      421 gtggacatat ataacctatg gagaaaaaca cgcatcaaat tgataaaagg gcgatatttc
      481 aacatgttgc agataatgtt atttttgata catacgcgtt ataaatatca aaaacacaaa
      541 ccagataaat atcttatcaa tatacaacaa agtttgaatt attcaattta tagcagcaca
      601 accaaacgcc aaaaaataat aaccaagagc tataaataaa gattaaaaat attttcagtg
      661 aatatgaaat tattgctatt tctattacaa agatgtcctg gtctctcttt cattctcttc
      721 ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt atactgtgca
      781 ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc ctcgtcgcct
      841 gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc ttaacgacct
      901 ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg aagccatgcc
      961 aagcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca tcgccaacgc
     1021 cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa ccggcagcga
     1081 cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc agactgtgcc
     1141 agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg gtcactgcgg
     1201 tgtgctgtgc gagcatggcg tcgacaacga cgcaactctg gaaaatttag gcaagcaagc
     1261 cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga tggatggcca
     1321 ggtacaggct attcgccagg cgctggacgc tgcaggcttt aaagatacgg cgattatgtc
     1381 gtattcgacc aagttcgcct cttcctttta tggtccgttc cgtgaagctg ccggaagcgc
     1441 attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg aggcgattcg
     1501 tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac ctgctggagc
     1561 atacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg gcgcgtatca
     1621 ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta tagatgaaga
     1681 gaaagtcgtg ctcgaaagct taggttcgat taagcgtgcg ggtgcggatc tgattttcag
     1741 ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt cctctcagaa
     1801 agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc ctctccccaa
     1861 gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt ttatccccaa
     1921 ggatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg gcatagtgct
     1981 gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa cgcacctgaa
     2041 actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc tctttctcgc
     2101 tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt ttaccgctca
     2161 ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc gcctcgcgcc
     2221 agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat ttacggaaat
     2281 catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc gcctcatagg
     2341 ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaactct ttagctgcca
     2401 gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag ttgtcgttat
     2461 ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct tcggcatgag
     2521 ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt tgctgctgcg
     2581 gcgcaatagt ttgatcgcgc agaaacacca cctgatggcg cagcaccgca tggtaaagct
     2641 gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt tgtgcgccga
     2701 tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc catgccaggg
     2761 cgtcagacgg cgctgtaacg cgcgcaaacc cagttctaaa agaaaggcga taatcgcaat
     2821 caccgcgatc cccgccagca ccacgtcagt cgcgagaaat tcacccgctg actgaaccat
     2881 aaatcctaaa ccgcgcgtcg cggcaatcag ctccgccgct accagcgtag accagcccac
     2941 acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca aaatgacaaa
     3001 ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc gaacctgctg
     3061 cgcgcttttc acccccgcca gcgccgacat cgccaccggt gcaaaaatcg ctaaatagat
     3121 cagtaagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg gcaaataagc
     3181 cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc gcaccgtcgg
     3241 gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga gtagcgccag
     3301 cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca taaatccctg
     3361 cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca aaaacagcgg
     3421 gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg ctaacgtgcc
     3481 aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc gcgaatgcag
     3541 tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac gcggcttaaa
     3601 acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct cgactctccc
     3661 gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacgcggcc agggccgggt
     3721 gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg ggtaatcaac
     3781 agcacctgct tgcccgtctc ctgccagagt ttcagcagca gggtttgcat ctggtcgcgg
     3841 gtgaaggcgt ccagcgcacc aaacggctcg tcgagtaata acagctgggg attcgccgcc
     3901 agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca gatatagcgt
     3961 ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc cagtcgctgc
     4021 attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac attgcgccac
     4081 ggtagcagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc ctcaatacgc
     4141 ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat cagattcagc
     4201 agggtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc gctttccagc
     4261 gtcaggttga tatcttccag tgccggtttg ccgccataat cggcgtaaag atgagagatt
     4321 tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg gctgtaatca
     4381 ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc gatgatcgct
     4441 ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata ggtattcccc
     4501 ttcaccagcc ccggaacgtc accttccggc acgccgctta aacgcgccag tttgctgatg
     4561 ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg agcatcgatg
     4621 gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc tttgcgcacc
     4681 acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc ggtcagcacc
     4741 ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc atcaatatct
     4801 ccccgctgcc aggcagcgat aatcgcgggc ggctgcaggt tcacaatctc cacttgcccg
     4861 ggtttaatgc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt agagataaac
     4921 ggtacggcga tgcgtttgcc aatcagatct tccggtttgc tgatagtttt ctttaccacc
     4981 agcgcttcgg agttacccag ttttgacgcc agcaagaaaa cttcaatcgg cacctgttgg
     5041 ctggctgcaa ccgctaacgg gctggaaccg aggttgccga tttgcacatc gccagaagcc
     5101 agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacggt tgctccgctt
     5161 tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga ggtttgatac
     5221 gcaacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc ggcaagaagt
     5281 gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg tgagcagtat
     5341 tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga ttttattctt
     5401 agcaaaaaaa cggcaaagga aagattaaat atgcaattac tccgttctaa caattgggaa
     5461 aattactaca cttcatttta gtgatgcata aacaaccaca ttacgagatt tattacttca
     5521 ttatttctat caatatcaat acatataata aaac
//
LOCUS       DAZKGS010000236         5408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKGS010000236 DAZKGS010000000
VERSION     DAZKGS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..566
                     /gene="hpaR"
                     /locus_tag="ACHOQY_004571"
     CDS             120..566
                     /gene="hpaR"
                     /locus_tag="ACHOQY_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFP9680522.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            685..2349
                     /gene="tsr"
                     /locus_tag="ACHOQY_004572"
     CDS             685..2349
                     /gene="tsr"
                     /locus_tag="ACHOQY_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9680523.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(2392..3663)
                     /locus_tag="ACHOQY_004573"
     CDS             complement(2392..3663)
                     /locus_tag="ACHOQY_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9680524.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(3660..4133)
                     /locus_tag="ACHOQY_004574"
     CDS             complement(3660..4133)
                     /locus_tag="ACHOQY_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9680525.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            complement(4197..5168)
                     /locus_tag="ACHOQY_004575"
     CDS             complement(4197..5168)
                     /locus_tag="ACHOQY_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFP9680526.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1428 a         1280 c         1381 g         1319 t
ORIGIN      
        1 agttaataaa acatgctcaa ttttgataaa atgaagaatt gcaggggcag cgcagtcccg
       61 gtaacgtgat gattaactgt ttctatatta atgattttga atttttcagg ggaaagataa
      121 tgcacgactc tctaaccatt gcgttgctcc aggcgcgaga agcggcgatg agttattttc
      181 gcccaatcgt taaacggcat aatttgactg agcaacagtg gcgcatcgtg cgtattctgg
      241 cggaaagccc atcaatggat tttcacgatc tggcgtatcg cgcctgcatt ttgcgcccaa
      301 gcctgaccgg gatcctcacg cggatggaac gcgacggttt agtgttgcga ttaaagccga
      361 tcaatgatca gcggaagctc tatatctcgc tgaccaaaga ggggcaggcg ctgtataacc
      421 gcgctcagac acagattgaa gaagcctatc ggcagattga agctcagttt actgccgaga
      481 aaatgcaaca gttaacccat ctgttagagg aatttattgc tctgggtaat tcccgacaag
      541 aagacatccc gggcgataat gaataacttt ttagtatctg ataaccagcg cctggttttt
      601 ccatggatgg cgggttacac cttttcataa agtttttgct ttccaggccg aaaatcttgc
      661 atcggtccac aggaaagaga aaccatgtta aaacgtatca aaattgtgac cagcttactg
      721 ctggttttgg ccgtttttgg ccttttacaa ctgacatcag gcggtctgtt ctttaatgcc
      781 ttaaagaatg acaaagaaaa tttcactgtt ttacaaacca ttcgccagca gcaatccacg
      841 ctgaatggca gctgggtcgc gttgttgcag acgcgtaaca ccctcaaccg cgcgggtatc
      901 cgctacatga tggatcagaa taatattggt agcggttcaa ccgttgctga gctgatgcag
      961 agtgccagta tttcgctgaa acaggcggaa aaaaactggg cggattacga agcgttgccg
     1021 cgtgacccgc gtcagagcac cgccgcagcg gcagagatca aacgtaatta cgatatttat
     1081 cacaatgcgc tggcggagct gatccaacta ttaggtgcag gcaaaatcaa cgagttcttt
     1141 gatcagccga cccagggata tcaggacggt ttcgagaagc agtatgtggc ttacatggag
     1201 caaaacgatc ggctctatga tatcgccgtc agcgataaca atgcctccta cagccaggcg
     1261 atgtggattc tggtgggcgt gatgatcgtc gtactggcgg tcatcttcgc cgtctggttc
     1321 ggtattaaag cctcgctggt agcgccaatg aatcgcctga ttgacagcat tcgtcatatt
     1381 gcaggcggcg atctggtgaa accgattgag gtggatggct ctaatgagat ggggcaactg
     1441 gcagagagtt tgcgccatat gcagggagag ctgatgcgta ccgtcggtga tgtgcgcaac
     1501 ggggccaatg ccatctatag cggtgccagc gaaatcgcta ccggcaataa cgatctctct
     1561 tcgcgcaccg agcaacaggc cgcttcgctg gaagagacgg cagccagcat ggagcaactg
     1621 accgcaacgg tgaaacagaa cgccgagaat gcgcgccagg ccagccatct ggcattaagt
     1681 gcttctgaaa cggcgcaacg cggcggcaaa gtggtagata acgtggtgca gactatgcgc
     1741 gatatctcca ccagttcgca gaaaatcgcc gatattatca gcgtaattga cggcattgcc
     1801 ttccagacca atattctggc tttgaacgcg gcggttgagg ctgcgcgtgc gggtgagcaa
     1861 ggacgcggtt ttgcggtggt cgcgggagaa gtgcgtaatc tggcccagcg tagcgctcag
     1921 gcggctcgtg aaattaaaag cctgattgaa gactcggtgg gaaaagtgga tgttggctct
     1981 acgctggtcg aaagcgccgg ggaaacaatg gcggagattg tcagtgctgt gacccgcgtg
     2041 acggacatta tgggcgaaat tgcttctgct tctgatgagc agagccgtgg tatcgatcag
     2101 gttggcttag cggttgctga gatggaccgg gtaactcaac agaacgccgc gctggtggaa
     2161 gaatctgccg ccgccgccgc cgcgctggaa gagcaggcca gtcgcctgac cgaagctgtg
     2221 gcagtgttcc ggattcagca acagcagcaa cagcagcgtg aaacatcggc tgtggtaaaa
     2281 accgtgacgc cagctgcgcc gcgtaaaatg gccgtggcag atagcgggga gaactgggaa
     2341 acgttttaac gcaatgacgc cggatgagta tttcatccgg cgtttttatt attaaaccag
     2401 gaacagtgaa atcgccggga tatatgtcac cagcgttagc attaatagta gagccgccag
     2461 tagaggcagg gattgtttaa agaaatttcc caccgaaact tgcgtgatgt tacataccag
     2521 cagcatcacg gtacctaccg gtggcgtgaa tgcgccaatc gacaggttga agatcatgat
     2581 gatgccaaaa tgcaccggat cgatgccaaa gtgttcgacc accggtttta atagtggtac
     2641 caggatcagc aaaatggcgt ttccttcaag aaacatcccc agcgtcagca gcaatatgtt
     2701 gataattaac aggaacatat atttattttc ggtcagcgaa gtcacaaaat tcgccactgc
     2761 gacaccagca tgttccattg agaaaatcca tcccaacgcg gcgctggcca tgattaccag
     2821 catcaccgat gcggttgcgc gtgccgtttc gcccaatgct ttgacgacat gagaaatccg
     2881 catttcacga tagataaaaa agccaatcgc cagcacgatt agcaccgctg ctgcacctgc
     2941 ttctgttggg gtgaaaatgt tagcgcgaat accacccacc acagcaaaaa tgaggaacag
     3001 cgcaggccag gcctgtccca gcgagaacac cacctctttg cccgtaggcc agctttcacg
     3061 cgagggttta gcgttagttt tgcgggcgac caggtagacg gtaaacatca gaacgactgc
     3121 gcatagcaaa ccaggtacca gcccagcgat aaacattttt ccgatagaga cgtcggcaac
     3181 cagaccgtaa ataatcaggg ctattcccgg cggtatgatc ggggtgacta acgagccacc
     3241 agcggtaatg gcggcggcaa acgcgcggtt gtagccttta cgctccatct ccggcaccat
     3301 catgcgcgac agcatcgccg cgtcagcaag gttagaggcg ctgacgccgc ccatcatggt
     3361 gctgacgaga atgtttgcca cgccaagtcc gccgcgcatt ttccccacca gaacgttggc
     3421 aatgtgcagg atgcgttcgg caatgccggt gtagctcatt agtgtgccga gcaagataaa
     3481 aaacggaatt gccagcaatg atgggctttg cgttggcgcg atcagtcttt gcacggcaat
     3541 ttcaatcggt acgctggaga agaataaaaa gtaagcgatg atgccaataa acatcgccag
     3601 atagacccga atgttgagaa acaaacagac gatcattagc ggaataatca ataaccaact
     3661 cattgcattt tgtctccgct atcagattgt tttatatcgt gtattaacca gtagagggta
     3721 taaatagccg ttgccagtgc accgacaggg accgccagat cgatccagaa ccaggaaacc
     3781 ttcagaagtt gtgtcacttt aaactgcgcc atttcggcta gtcggtagcc cagccaggcc
     3841 atatacagca gcagtgcgat ggagagaacg gcgaccagta ctgcaataac acgcctgagc
     3901 ttgcctggaa gcatctccat caggaaggga atggtcaggt gcgccctgtc acgttccgcc
     3961 accactccac ctaacatcac cacccaaatc atcagcattc ccgacatttc ttctgtccat
     4021 tgaagcggtt ggccgatgaa ataacgcata aataccgctc caattgtcat cacaaccaga
     4081 acgaatagcg caaaggcagc actaaattct gccactttgc caaaaagacg catgaaacct
     4141 cctgttagtg cgtgtcctgc ctaagagaga tgaggcagga cgctgagcat taatgcttat
     4201 tgcagttgct gttgaatggt ttcgtacaga cctggtgtcc attccgggaa ttgagagtaa
     4261 acttcgcgtg ctttcttctg gaatggtcca gtatcgggat agatcacttc gacgcccgct
     4321 gcctgcatgg ttttcaacat ttcggcatca cgttcaagag tgagtttctg gctatagaca
     4381 cccgcttcat agccggtctg gtggatcatc tccagttgtt ctggtgaaag ggtgctaaag
     4441 aaggcttcac ctcctatcca tactgaggta tttgtcaggt agttaaccat tgtcagatat
     4501 cttgcctgct catacagttt ttgcccttgc agcactgaaa ttgggttttc tacgccgtca
     4561 ataacgccct gggttaatgc gggataaact tcgcctaatg gcatgggagt tggtgttgcc
     4621 cccatcgcct gaatggcttt tatttgcatg acgttattag gaacacgaat ttttaagcct
     4681 gcgagatcgt caacagtgcg aatgggtttt ttcgaaataa tctgtcgagt gccatagaga
     4741 taattattca ttaccacatg aattcccttt ttcttcagac tttcattttt ttgtttaaac
     4801 cagtcgcttt catatatttt gaacagtttt tgcggatcgt cagtcagata cggtccaaac
     4861 aaaataccca aatcgggttc ataatcggca agaaaggcca catcactcaa ggttatgaca
     4921 ttcatgccca tcatggcttg ctcggtgaca tcctgttttg agcccaattg ggaacttgga
     4981 tagagcgcaa gtgttatttc gccattactt tttttattta ataggtctgc ccagtaacgc
     5041 atgaccacat ccaggggttc tccagggtta ttttcatagg caacttttat cgaaataggt
     5101 tttgcctgga ttattgacgg taaaaaaagg gtactgacag taattagcat cgctattatt
     5161 ttgctcatct ttacttactc catgctaagg aaataaaact ccaacattgt tgtcgggagt
     5221 gaaaccttat gaaaaagcag tcattactga aaagtagtca gcgaatagcg gaatggctgc
     5281 aaatgtgtca taaattcgtg caccgggtaa tactttgctg cttttaatcg gtttttaatg
     5341 cattttttga ttgtatatct taaagttatg taatgtatga cgttacaagg aacacccact
     5401 gatgagtt
//
LOCUS       DAZKGS010000237         5266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKGS010000237 DAZKGS010000000
VERSION     DAZKGS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..545
                     /gene="ygiL"
                     /locus_tag="ACHOQY_004576"
     CDS             <1..545
                     /gene="ygiL"
                     /locus_tag="ACHOQY_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9680527.1"
                     /translation="FKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGD
                     EDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQ
                     TDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESP
                     SKDATPGNVYAQADYKIAYE"
     gene            605..3109
                     /locus_tag="ACHOQY_004577"
     CDS             605..3109
                     /locus_tag="ACHOQY_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9680528.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3125..3874
                     /gene="yqiH"
                     /locus_tag="ACHOQY_004578"
     CDS             3125..3874
                     /gene="yqiH"
                     /locus_tag="ACHOQY_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9680529.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3876..4941
                     /locus_tag="ACHOQY_004579"
                     /pseudo
     CDS             3876..4941
                     /locus_tag="ACHOQY_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4984..5184)
                     /gene="glgS"
                     /locus_tag="ACHOQY_004580"
     CDS             complement(4984..5184)
                     /gene="glgS"
                     /locus_tag="ACHOQY_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9680530.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT         1626 a         1099 c         1072 g         1469 t
ORIGIN      
        1 cttttaaaaa gtcacttctt gttgctggcg tagcaatgat tctttccaat aacgtatttg
       61 ctgacgaagg tcacggcatt gttaaattta aaggtgaagt tatctctgct ccatgttcaa
      121 ttaaaccagg tgacgaagac ttaactgtaa atctgggtga agtagcagat accgttctga
      181 aaagcgacca gaaatctttg gctgagcctt tcactattca cctgcaagac tgcatgttga
      241 gccagggtgg aaccacttat tcaaaagcga aggttacttt caccacagcg aatactatga
      301 cgggtcagac tgacctgctg aaaaacacca aagaaactga aatcggtggt gccacaggcg
      361 ttggcgtacg tattctggat agccagagcg gcgaagtgac tttaggtact ccggtagtaa
      421 ttacattcaa caatactaac agctatcagg aactgaactt caaagctcgt atggaatccc
      481 caagcaaaga tgctaccccg ggcaacgttt acgctcaggc tgactataag attgcttacg
      541 agtaatcaca ttaggccggg ttcgcccggc cacatcatta acggattaat gataagtgga
      601 acagatgtat aaaaaattaa aattaaccac aataagcgaa ttgattaaaa atatttattg
      661 ttcattatcc gttctcatca ttggttgtgc gtcagcttat gccgttgaat tcaacaaaga
      721 tttaatcgaa gccgaagatc gtgaaaacgt taacctttcc caatttgaaa ctgatggcca
      781 attacccgta ggtaaatact cattaaacgc tcttattaat aataaaagga cgccaatcca
      841 tctggacctg caatgggtat taattgataa ccaaactgca gtttgtctga ctccagagca
      901 attaacatta ttaggcttta ctgatgagat tattgaagag gctcagcaaa acctgatcga
      961 tggttgttac cctatcgaaa aagaaaaaca aattacaact tatctcgata aagggaaaat
     1021 gcaattatcc atatctgcac ctcaggcatg gttaaaatac aaagatgcaa actggacgcc
     1081 tcctgaactt tgggatcatg gtattgctgg ggcatttctt gactacaatt tatatgcctc
     1141 tcattatgca ccacatcagg gcgataattc acaaaatata agttcctatg ggcaggctgg
     1201 ggttaatctt ggggcctggc gcctgcgtac tgattaccag tacgatcagt catttaacaa
     1261 tggcaaaagc caggcgaaca acctggattt tccgcgtatt tatttgtttc gcccaatccc
     1321 agcaattaat gcaaaactaa ctataggtca atacgatact gaatcctcta ttttcgactc
     1381 tttccatttt tctggcgttt cgttgaaaag cgatgagaat atgttgccgc ctgatctacg
     1441 tggttacgca ccgcaaatca cgggtgttgc acaaacgaat gcaaaggtca ctgtctcaca
     1501 gaacaaccgt attatttatc aagaaaatgt tcctcctggc ccatttgcta ttactaattt
     1561 attcaataca ttacaggggc aactggacgt caaggttgaa gaagaggacg ggcaagtcac
     1621 gcaatggcaa gttgcatcta atagtattcc ttatctgacg cgtaaagggc agattcgcta
     1681 caccactgct atgggtaaac cgaccagcgt tggtggtgat tccttacaac aacccttctt
     1741 ctggactggt gaattctcat ggggttggct gaacaatgta tccctgtatg gtggttcagt
     1801 tttaacaaac cgtgattatc aatctctggc tgccggcgtt ggctttaacc ttaactcatt
     1861 gggttcatta tcttttgatg tcacacgatc tgatgctcag ttgcataatc aggataaaga
     1921 aacgggttat agctaccgcg ctaactattc aaaacgtttt gaatctaccg gtagccagct
     1981 cactttcgct ggttaccgtt tctctgataa aaactttgtg acaatgaatg aatatatcaa
     2041 tgacactaac cattacacga attatcagaa tgaaaaagag agttatattg tcacgtttaa
     2101 ccagtatctt gaatcattaa ggttaaatac atacgtaagt ttggctcgta atacttactg
     2161 ggacgccagc agtaatgtga attattcatt atcacttagc cgcgattttg atatcgggcc
     2221 attaaaaaac gtctccactt ctctaacatt tagccgaata aactgggaag aagacaacca
     2281 ggatcaactg tacctaaata tttcgattcc ctggggaacc agtagaacat tgagctatgg
     2341 tatgcaacga aatcaggata acaagatttc gcatactgct tcgtggtatg actcttccga
     2401 tcgaaataat tcctggagcg tttctgcttc aggcgacaat gatgaattca aagatatgaa
     2461 agcgtcacta cgcgccagtt atcagcataa taccgagaac ggtcgtctct atctctccgg
     2521 gacatcacag cgagacagtt attattctct gaatgccagt tggaatggtt cattcacggc
     2581 gactcgtcac ggagccgctt tccatgacta tagtggtagt gctgactcgc gttttatgat
     2641 cgacgcagat ggtgcagaag atattccgtt gaacaataaa cgcgcggtaa ctaatcggta
     2701 tggcatcgga gttattccat cagtcagcag ttacataaca acatcattaa gcgttgatac
     2761 ccgaaatctt ccagaaaatg tggatatcga aaactcggtt atcaccacca ccttaaccga
     2821 aggtgctatt ggctacgcca aacttgatac ccgcaagggc tatcaaatca tgggggttat
     2881 tcgcctggca gatggtagtc acccaccact ggggattagc gtaaaagata aaaccagcca
     2941 taaagaatta ggactggttg ctgatggcgg ctttgtatac ctcaacggca ttcaggatga
     3001 cagcaaactt actttacgct ggggtgataa atcctgtttt attcaaccac ccaatagcag
     3061 caacttaacc accggaacgg ttattttacc gtgtattagc caaaattaat tcaggatgaa
     3121 actaatgcgc tacttaaaca caaagaatct cattgcagca ggagtattac tcgcctgcat
     3181 gagcagcatt gcctggggtg ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc
     3241 agataaaggg gaagcattaa aacttactaa tcaaagcaaa aatctaccct atctggcgca
     3301 aacatggata gaagatacga aaggcaataa atcacgtgac tttattgtca cggttccccc
     3361 tatggtacgt ttaaatccca gtgagcaaat tcaaataaga atgataagtc aggaaaaaat
     3421 tgctcaactg cctaatgaca gagaaacgct cttctatttt aacgtgcgag aaataccgcc
     3481 aaaaacagat aaaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg
     3541 gcgaccaaaa gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat
     3601 catccgtaga aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct
     3661 gggttatatc ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac
     3721 cccttatagc catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta
     3781 tatcaatgat tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc
     3841 ttgtaatgtt tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta
     3901 tatttattat aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg
     3961 aagggggaaa taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta
     4021 aaaaaggtga taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg
     4081 cattacctgc cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt
     4141 ttttaccagg cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca
     4201 atggagttga ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata
     4261 taaaaggagc gggtaccttt aataatcact ataaaaaacc ccgctgtctg tagtggaggt
     4321 cctgagcctc aattatcagt aacctttcca gctcgcgtac aactctatat taaattagca
     4381 aaaaatgcca atagagttaa taaaaacctg gtgttacctg atgaatatat cgctctagag
     4441 tttaaaggta tgagtggcgc aggagctata gaagtagaca aaaatttgac cttcaggatt
     4501 cgtggattaa ataatattca tgtccttgac tgctttgtta atgtcgcact ggaaccagct
     4561 gatggcgttg ttgactttgg taaaataaat tcccgaacaa ttaaaaatac cagcgttagt
     4621 gagacgttta gtgtagtcat gacaaaagat cctggtgcgg cctgtactga gcaattcaat
     4681 attttaggga gctttttcac tacggatatt ttgagtgatt atagccatct ggatatgggt
     4741 aatggactgc tattgaagat atttcataaa gatggaacag caacggaatt taaccgcttc
     4801 tcacaatttg cttctttttc atcgtctagt gcaccttcgg tcaccgcacc attcagggca
     4861 gaacttagtg cgaatccggc agagactgtt gttgagggac cgtttagtaa agacgtcatc
     4921 ctcaaaatca cctataacta gcatctaata caaacactaa aacgggccat caggcccgtt
     4981 atatcagtgc tctaactcca gctctcttgc ctgcatcatc tgttgacaat accaggcata
     5041 acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg caagaatatc
     5101 gaccgggcga ccttctgcgt gcattctggc aaaactacgc gccaggaaat cgaaattatt
     5161 taaagaatta agactatgat ccataagcac atcctccggt tatcgcatac cttttaaggc
     5221 catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcct
//
LOCUS       DAZKGS010000238         5180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKGS010000238 DAZKGS010000000
VERSION     DAZKGS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..204
                     /locus_tag="ACHOQY_004581"
     CDS             <1..204
                     /locus_tag="ACHOQY_004581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680531.1"
                     /translation="GVAEQKTCCIVKRNPRRHVHTPQAGYLTPDHCRVTRSIALRSVG
                     RGGFMDTIYKLKFNWSTCLLLNL"
     gene            250..552
                     /locus_tag="ACHOQY_004582"
     CDS             250..552
                     /locus_tag="ACHOQY_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP9680532.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            555..914
                     /locus_tag="ACHOQY_004583"
     CDS             555..914
                     /locus_tag="ACHOQY_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680533.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(961..1353)
                     /locus_tag="ACHOQY_004584"
     CDS             complement(961..1353)
                     /locus_tag="ACHOQY_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680534.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1480..1740
                     /locus_tag="ACHOQY_004585"
     CDS             1480..1740
                     /locus_tag="ACHOQY_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9680535.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1737..2174
                     /locus_tag="ACHOQY_004586"
     CDS             1737..2174
                     /locus_tag="ACHOQY_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9680536.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2261..3271
                     /locus_tag="ACHOQY_004587"
     CDS             2261..3271
                     /locus_tag="ACHOQY_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP9680537.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3264..3725
                     /locus_tag="ACHOQY_004588"
     CDS             3264..3725
                     /locus_tag="ACHOQY_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9680538.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3759..4484
                     /locus_tag="ACHOQY_004589"
     CDS             3759..4484
                     /locus_tag="ACHOQY_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9680539.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            4500..4892
                     /locus_tag="ACHOQY_004590"
     CDS             4500..4892
                     /locus_tag="ACHOQY_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9680540.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            4889..>5180
                     /locus_tag="ACHOQY_004591"
     CDS             4889..>5180
                     /locus_tag="ACHOQY_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9680541.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVA"
BASE COUNT         1415 a         1114 c         1436 g         1215 t
ORIGIN      
        1 ggggtagcgg aacaaaaaac ctgctgcata gttaaacgta atccccgccg tcatgttcat
       61 acgcctcagg ctggctactt aacccctgac cactgccggg taactcgaag tattgccctg
      121 cgttctgtgg ggcggggtgg gtttatggat acaatttaca aattaaaatt taactggtca
      181 acatgtttat tattaaattt gtaattgcgg gctgttgcat cagtcccaaa atgggactac
      241 tatacagtta tgaagattat ctcagttaaa acactcaggg atttttgggc ggagaatcct
      301 gatgcagaac aaccgctaag ggcatgggtg gatgaggcgg caaaagctga ctggaaaagc
      361 ccagcagaca ttaaggcaca ataccgaacg gccagtatat tgaaaaaccg gcgcgtggta
      421 ttcaacataa aaggtaatca ttaccgttta attgttgcaa ttgcgtatca gcgaggatgg
      481 gtatttgtta aattcatcgg aaaccacaag gaatacgatg ctattgatgc tgataccatc
      541 gaactggagt aagcatgaac atcaaaccta ttcgtacaga gcaagattat gaagccgcgt
      601 tgcgtgctgt tgaacccatg ttcgacaatg agcccgaaat tgacacgcct gagggggatt
      661 tttttgaggt gatgtgtttg ctaatagagg aatatgaaaa aaaacattat cccattgagc
      721 caccatcccc aattgaagct ataagatttc gcatggaaca gcaggggctg actgtgaaag
      781 atttggaacc cgcaattggg aaaaaaaatc gggtttatga ggtgttgaat ggtaccagaa
      841 gccttacgtt accaatgatt cgccgtcttc ataatcaatt tggtatcccc ctggaaagcc
      901 tggttggatt ataaaatctg ctagtcattt gcctgatgct cgttccagaa aaggaatgca
      961 tcaggcagtt ttgtttttct gccgcagtaa ctcttcaagt ttccgtttat agaaatcgcg
     1021 tttttgctcc atatcacgaa tgatctgctc tgcgtcgctt tgaggtaact catctaaaag
     1081 cgatatgatt tttcgttgtt gttctgtaag ttgcggttgg ttgtcattac tggatacagc
     1141 cattttatcg ccgagagttt cttcttccat aaagaaccaa tggacgggat gttgtgagag
     1201 ctctgctaat ttttccagtt tatccattct tggcatcacg cctttcaacc aaccttgcac
     1261 ggattggggt tttacaccaa gacgtcttcc cagctctgac tggtttatat tcaattcctg
     1321 caacacctgc tgaaggcgtt ttacaaagat catcaccacc cctcgtaaac tagttccgcg
     1381 atcctacaga aaaaattgat aagtggcatt acaaatagaa gttgaaattt aaatttaaat
     1441 ttgtaattat cggtgtcatc gtaaagttcg gagggaaaca tgcaaaaaag tactcaagtg
     1501 aaaatcctgt caataatgag ccaatcagaa ttagggcgtc gtcttggtaa aacaccgcaa
     1561 accataagtg ggtggtttaa aaaacgagtg ccagcggagg aggttatacc agcatgtgag
     1621 gcgcttgact ggggagtaac tccgcatgaa ttgcgccctg ataaataccc taatccaacc
     1681 gatggtttac ctgttgagta tcaggctaac gcacaagcag cggcgggagt tgattcatga
     1741 aaatcaagca tgagcacatc cgcatggcga tgaatgcgtg gctgctttat ccgagggtag
     1801 ggcgcaaaaa aatcgctgat gatatagcga cagcatattt tgagcttgaa atgacttatc
     1861 caccaatgca tgacacctct acgacagagg gtattggatt gaacatacaa aatatttttc
     1921 gctggcttga aaaggatacg cctgatgctg ttgaaaaaat tcaggcacta attccagcta
     1981 tcctgactgt tcttccgcgt gaactgcgtt atcacctcag tatttttgac actgttgagc
     2041 gccgtgcatt actggcggcc caggaagcgt tgagcacggc aattgatgcg catgatgatg
     2101 cagtccaggc agtttaccgg aaagcgtatt tcagcgacgg cgggtcatcc ggcgagtctg
     2161 ttgtggtgca ttgatattta tgccagaccc ctgctgattc tgttgattgg ggaatcacag
     2221 aatatcacca gaggatggtt cgtcacaaga tgaggcaatt atggccgcat taccatacat
     2281 gcaactgtac atagctgatt acctggctga caccatgcat ttgtccgcag aggagcacgg
     2341 tgcgtatttg ttgctgatgt tcaattactg gcaaacaggg aaaccaatac ctaaaaacag
     2401 gctggcaaaa atttcccgtc tgactaacga gcgatgggct gatgtggaac catccttgcg
     2461 ggagtttttt tgcgataaca gcgatgaatg ggtgcatctt cggattgagg aagatctggc
     2521 atcagtcagg gaaaaattga ccaaaaaatc agccgccgga aaagcatctg ttcaggccag
     2581 aagaagcaga aaggaagcat atgttcaaac aaaacaagag agagatttaa caggtgttca
     2641 aacaaatgtt ggtgttgtgt ttgaacatga cgcaaacaca aaagcaacta ataaagatac
     2701 agatctaaaa gaattaaacc ccacacataa cgtgcgtgaa cgcgagagta ttccgaccag
     2761 tgagtcgcat ggtgcgccgt tgcagacagc cgaacctgaa tacctggacg gcctgagcga
     2821 accgatcggg aaattttcga tgactactgt ctggcagccg tcgccggatt ttcgacaacg
     2881 ggcagcagtg tggggtatgg ctctgcctga gccggaattt acacctgctg agcttgccgc
     2941 attccgggat tactggatgg cggaggggaa ggttttcacg caggttcagt gggagcagaa
     3001 atttgcccgc cacgtgcagc acgtcagggc acaggtaaaa ccagtcagca agggggtaag
     3061 ccatgcagca tcaggtggca cggcatcacg ggcagttcag gaaatccggg cagcacgcga
     3121 acagtgggaa cgtgaaaacg gatttatcag caacggaaac ggcctggaag ctgtgggagc
     3181 ttatggggga ggtgtattcg aaccgctgga ctcagaagaa cggggccgca ccttcgaagc
     3241 tctggattgc ccagattggt gcgatgactg aacagcaaat ccgtctggtc tgccgtcagt
     3301 gcatggaccg ctgccgggcg ggtgaaacgt ggcccccgga cctggctgag tttgttgcgc
     3361 tgatttcgga gagtggggca aatccatttg gtcttacggt ggatgccgtg atggaagagt
     3421 accggcgctg gcgcaatgaa tcctggcgat acgacgggag tgataaatac ccgtggccac
     3481 agcctgtgct gtaccacatc tgcctcgaaa tgcgtaccag agggattgag cgccagatga
     3541 cgcagggtga gttaaaacta cttgcagaac ggcaactgac gaaatgggca aagcatgttg
     3601 gtaacgggat gagtgttccg ccagtgcgac gacaactgga aggggcgaaa cacccgcaag
     3661 ggccaacgcc aattgagcgg ctgaaacagg aatacgaacg ccggaaggca gctggtttta
     3721 tttgaatctg agaaacgatt ttgtcggagg aaattttaat ggaaaccgta tttgacgcac
     3781 tgaaagcact gaaaagagcc tcttcacagg tagtggcggc ccgccttgga atcagccgtg
     3841 aagatgcggt caacgaactg tggaaactga agcgccgcgg tgaagcggat aacaaggggt
     3901 cgatgtggtg gctgattcag gctggtgaaa gtgaaccggt gtcaccggta ccgaaagtga
     3961 cagcgcaaat gctgactgag gcgattgaac aacatggccc acaaacggcg gatgagctgg
     4021 cactgatgtt cgggattacc tcccgccggg cgaattcatc gctggccatg gtaatcagca
     4081 aagggcgtct gattcgcgtg aatcagggcg gtaaatttcg ttactgcata ccgggcgctg
     4141 atttaccggc agagccggaa gctgcatccg tagcggaaac cgatggtaaa gcctttcctc
     4201 agccagcagg tgttgcgtta ccagttcggg aagcagaaac acaggaagaa ataaaaacgg
     4261 aaagtgtggc ggtcacagtg cagtcacagc cgtcgttcac cagaaaacat ccggatggtc
     4321 tgattttacc atcgctgcat gtggctaacc gcgagctgcg ccgggcaaaa ggtcaggttc
     4381 agaagtggga gcgagtctgc gccgcgctgc gggagctgaa caagcaccgg gatattgttc
     4441 gacagattac tgattcttcc cgccgtgttg tatcggaaaa gtgattgccg gaggcgctta
     4501 tggcaaaaga atttacacca gaagagcggg aagaagtgaa ggcgcgcatt gtggaattcg
     4561 tgcgcctgag cggacgagaa acttttcgac aactggcaga taaaacgggt gtcagtaaga
     4621 ccgctattcg tcgtttatct ggtgcgcttg cggccagtgg tgatgtctgg ctctctggtt
     4681 gcggggtatt tccatcagag caggcgtatc gcgtatggcg taagacaccg gagaaggctg
     4741 ctgacccgac actgattcga aagttacctg acggagaaat acgtcgttac aacagacggc
     4801 agaacataat ttgtcgtgag tgccgccaga gcgaagttat gcagcgtgtg ctggcgttct
     4861 atcggggaaa ctttcaggag gtgatggagt gagggtgaga gtttatattg ccggtccaat
     4921 gacgggatat gaaaatttca accgtgaggc gtttcacaag gcggaagagg aactgaaacg
     4981 ggaagggcat accgtcttaa ctccggcagt acttccggac gggctgacac agccgcacta
     5041 catggatatt tgcatggcaa tgattcgttg tgtggatgcg atttacatgc tgaatggctg
     5101 gcagcggtca gcgggcgcta aggcagagct ggcactggcg gagaaactgg ggcatgcggt
     5161 gatttatcag gaggtggctc
//
LOCUS       DAZKGS010000239         5125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKGS010000239 DAZKGS010000000
VERSION     DAZKGS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..358)
                     /locus_tag="ACHOQY_004592"
     CDS             complement(<1..358)
                     /locus_tag="ACHOQY_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9680542.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHOQY_004593"
     CDS             complement(625..1290)
                     /locus_tag="ACHOQY_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFP9680543.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHOQY_004594"
     CDS             complement(1431..2072)
                     /locus_tag="ACHOQY_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFP9680544.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2774..3805
                     /gene="repA"
                     /locus_tag="ACHOQY_004595"
     CDS             2774..3805
                     /gene="repA"
                     /locus_tag="ACHOQY_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFP9680545.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
     gene            complement(4765..>5125)
                     /locus_tag="ACHOQY_004596"
     CDS             complement(4765..>5125)
                     /locus_tag="ACHOQY_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680546.1"
                     /translation="NGKDGVAQMSLPIYAYSTCRFWPKESVAVGDTINLSNYEHIEFS
                     FNSFSKDVCREQNSDIYKIFFKIVALNNSIVVSENMFTQKNFLEHNTFFSVTGIDESL
                     YEKHIIFITLKAIPPQK"
BASE COUNT         1392 a         1241 c         1325 g         1167 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataag gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtag
     2281 atcgtaatca caagatcgat tcatccaaaa aacatgaaaa acacagcata aaagatcgtt
     2341 tgtggaattg tctatttttt gacacagttc aaaaaaacga cgttttttag gtggttaatg
     2401 agaggcctgg taaatcagag aaatcgtgtt ggcttttcaa agcggtggaa aaggggtata
     2461 ttgcggatcg ttatgcagtg gcttttggga tcgtcaggat ccgggaagtc agaaaatggc
     2521 aggatgcgcc ataaggcatt caggacgtat ggcagaaacg acggcagttt gccggtgccg
     2581 gaaggctgaa aaaagtttca gaaggccata aaaggaaaac ccccataatc ttctcatcga
     2641 acttggcgga acgttagaag attatggggg cctcacagaa tacgggataa gtatatatga
     2701 aaccgtgtca gagattcaac ccttcgcagt gtattaacgc acggcattct gtttccgcca
     2761 taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac actggagtca
     2821 gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa gggcgacctc
     2881 tttcctggtt gaaccggaaa ggaagcgcac caggcgccgt cgtggcgaac actccactaa
     2941 acccaaatgc gaaaatccgt cctggtatcg tcctgcgcgc tataaggagc tgagcgggca
     3001 gctcgggcac gcctacaacc gtctggtgaa aaaggccccg gtgaccggcg agcagagcct
     3061 gcgtatgcgt atgtcgcggc atccttttta cgtgcagaaa cggacgttcg tcggtcgcaa
     3121 atatgctttc cggccggaaa aacaacgcct cctcgatgcc atctggccgg ttctggtcag
     3181 cttcagtgat gccggcacac acactgtggg gatgagtgtc actcgcctgg ctaaagaaat
     3241 cagtccgaaa gacagtaagg gaaatgttat tccggaactg gaagtgacgg tctcccgtct
     3301 ttcccgtctg ctggccgaac aggtgcgttt tggtgtgctg ggtgtttcag aggaaacgat
     3361 gtgggaccgt gaaacccgcc agcgtctgcc gcgctatgtc tggataacac cggctggctg
     3421 gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag cagcagaaac gactgcgtga
     3481 aagtgaaatc cgccagcagc tcattcggga aggtgttctg cgtgaggatg aagatatctc
     3541 cgtacatgcg gccagaaaac gctggtatct gcagcgcagt caggatgcgc tgaaacaccg
     3601 tcgtgcgaaa gcagcagcca gtaagcgcgc cagacgcctg aagaaactgc ctgccgacca
     3661 gcagattcat gagatggcag agtatctcag gaagcgtctg ccgccggatg aagcctattt
     3721 ttgttccgat gaccatctga agcgactggc catcagggag ttgcgtcagc ttgaactgac
     3781 gctggctgcc ccgccaccgc actagacagc accattccct cagcactgaa tcatcgccag
     3841 cccctccggg gctttcggcg caggtatcgc tcagcccaaa atccgcagta atcaccttaa
     3901 atcccctcag aggggcatat ttgctcataa aaccacgcat cagtcatcag aacgtggcca
     3961 cgttgtttca gttatccaca taaatccgca aacaaagaac tttaagaagc tgcaaacctg
     4021 aaacagcaaa cctgcaatat agtcttaacc ccattattta atcccctgcg ttgcttcgcc
     4081 gcagcgaaag tctttatctc tgaaaccact gtgaacaaac acaaagaggc cttcgcttgc
     4141 agcggccaag gccgcgccgc tcagaatcta aaagcacctc ccacgctgac gcgcgggccc
     4201 cgaacctcac cgttctggaa ccacaacaaa aaaacatcag gaataaaaac accacacaaa
     4261 cgcagcaccg tgcccacccc tcataactga aaagcgccgc cgccccggcc caaagggccg
     4321 gaacagagtc gcttttaatt atgaatgttg taactaaaca tcatcatcgc tttaagaccc
     4381 catttgtcta gcagggcgat ggcccactgc gtgaaccatc accctgcaca cagggtattt
     4441 tatgtgcaat tgtcaaccac tgaatcggaa ccccagaggg gcccccacga ttcagaagtt
     4501 gacaatttaa gcacagggac tacatgagaa aaatcgtggt tcgtctgtca tacacgcgcg
     4561 taagcggcac tgataaccct cttacgcgga aatacgccca taagcgctaa cttaagggtt
     4621 gaaccgacgc cacggtgtct cgttaagact tcaattgcgt tttgtaggca gtcagggata
     4681 ctgtcccatg aatggccacc tgtaagctcc agataaccat ttttgttatt ctccacaacg
     4741 aattccgcat accggaaatc agcgctactt ctggggagga atggccttaa gagtgataaa
     4801 gattatatgc ttttcataga gagactcatc tatgccggta acactgaaaa atgtattgtg
     4861 ttccaagaag tttttttgag tgaacatatt ttcgctcaca acaatcgaat tattcaaagc
     4921 cacaatttta aaaaatattt tatatatatc actattttgt tcacgacaga catctttact
     4981 aaaagaatta aagctaaatt caatatgttc ataattagat aaatttattg tatctccaac
     5041 agctacactt tcttttggcc aaaaacgaca tgttgagtat gcatatatag gtaatgacat
     5101 ctgagctaca ccgtcttttc catta
//
LOCUS       DAZKGS010000240         5123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKGS010000240 DAZKGS010000000
VERSION     DAZKGS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..834
                     /locus_tag="ACHOQY_004597"
     CDS             <1..834
                     /locus_tag="ACHOQY_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9680547.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHOQY_004598"
     CDS             876..1271
                     /locus_tag="ACHOQY_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9680548.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHOQY_004599"
     CDS             1283..1657
                     /locus_tag="ACHOQY_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9680549.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHOQY_004600"
     CDS             1648..2226
                     /locus_tag="ACHOQY_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680550.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHOQY_004601"
     CDS             2223..2618
                     /locus_tag="ACHOQY_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9680551.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHOQY_004602"
     CDS             2626..3366
                     /locus_tag="ACHOQY_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680552.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHOQY_004603"
     CDS             3382..3804
                     /locus_tag="ACHOQY_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9680553.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHOQY_004604"
     CDS             3786..4220
                     /locus_tag="ACHOQY_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9680554.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHOQY_004605"
     CDS             4213..>5123
                     /locus_tag="ACHOQY_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9680555.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1133 a         1313 c         1642 g         1035 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cggcatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZKGS010000241         5083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKGS010000241 DAZKGS010000000
VERSION     DAZKGS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHOQY_004606"
     CDS             <1..75
                     /locus_tag="ACHOQY_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9680556.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHOQY_004607"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHOQY_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9680557.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1543
                     /locus_tag="ACHOQY_004608"
     CDS             863..1543
                     /locus_tag="ACHOQY_004608"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9680558.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1540..1722
                     /locus_tag="ACHOQY_004609"
     CDS             1540..1722
                     /locus_tag="ACHOQY_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9680559.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            1695..1886
                     /locus_tag="ACHOQY_004610"
     CDS             1695..1886
                     /locus_tag="ACHOQY_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9680560.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            1963..2178
                     /locus_tag="ACHOQY_004611"
     CDS             1963..2178
                     /locus_tag="ACHOQY_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9680561.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2495
                     /locus_tag="ACHOQY_004612"
     CDS             2277..2495
                     /locus_tag="ACHOQY_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9680562.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            2543..2821
                     /locus_tag="ACHOQY_004613"
     CDS             2543..2821
                     /locus_tag="ACHOQY_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680563.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            2793..2858
                     /locus_tag="ACHOQY_004614"
                     /pseudo
     CDS             2793..2858
                     /locus_tag="ACHOQY_004614"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3020..4183
                     /gene="intD"
                     /locus_tag="ACHOQY_004615"
     CDS             3020..4183
                     /gene="intD"
                     /locus_tag="ACHOQY_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9680564.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4199..4275)
                     /locus_tag="ACHOQY_004616"
     tRNA            complement(4199..4275)
                     /locus_tag="ACHOQY_004616"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4239..4241),aa:Arg,seq:tct)
     gene            4518..>5083
                     /gene="fimZ"
                     /locus_tag="ACHOQY_004617"
     CDS             4518..>5083
                     /gene="fimZ"
                     /locus_tag="ACHOQY_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP9680565.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNI"
BASE COUNT         1505 a         1159 c         1251 g         1168 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggcaggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gttgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcattg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gtacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gaatggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacggcata
      541 aagccatgat tcagtgtgcc cgtctggcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aatactgcat acactgcaga acgtcagcca gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg agattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgcg
      781 catcgtcaga actgacacag gccgaagcag tgaaagctct tggattcctg aaacagaaag
      841 ccactgagca gaaggtggca gcatgatacc ggacattatc ctgcagcgta ccgggatcga
      901 cgtgagagct gtcgaacagg gggatgatgc atggcacaaa ttacggctcg gcgtcatcac
      961 cgcttcagaa gttcacaacg tgatagcaaa gccccgctca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taatgctaag gcgctggcct ggggaaaaca gtacgagaac gacgccagaa ccctgtttga
     1141 attcacttcc ggcgtgaata ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac tgaaatgccc
     1261 gtttacctcc cgggatttca tgaaattccg gctcggtggt ttcgaggcaa taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacgcga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatttgggga gcaatggcga tgacgcatcc tcacgataat
     1561 atccgggtag gcgcgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatcagaaa tccactgaaa gcacagcggc tggctgagaa gataaataat
     1681 aaacgggagg cggtatgcac aaagcatctc ctgttgagtt aagaacgagt attgagatgg
     1741 cacatagcct tgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggtg gcgaaagcag
     1861 aagcagatga gagagaccag gtatgacaac cactgaatgc atttttctgg cagcgggctt
     1921 catattctgt gtgcttatgc ttgccgacat ggggcttgtt caatgacacc tcagcaagaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcat
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac taaagaagca ccgcgaaacg
     2101 gtaacgctga tgggattcac accgactcat ttaagcctgg cgatcggcat gttaaacggc
     2161 gtctttaagg aacggtgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcatcctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaatcg aagaattaca gcgcaataca gcaataaaaa
     2341 tgcgtcgtca gaactaccag actgtatccg caactcattg ttgtgagtgt ggcgatccga
     2401 tagatgagcg aagacgcctg gctgttcagg gttgtcggac ttgtgcaagt tgccaggagg
     2461 agatcgaact taagaacaaa caatggggac tgtgatggcc tcaaagcagc aaatttcaac
     2521 atcgtccaac tgaggtgtaa aaatgttcag aatcattttt cctaacacct ggtacgtcga
     2581 ccaccacggc actccctgca aaatcctgcg ttctacccac aacaaagttc actacatccg
     2641 aaaaggcaga acatgtatcg ccagcatgtt ccgctttaat catgactttg aacctgtgaa
     2701 taaagctgat gcagatcgga tagcagaaga gatcgaaacg gcagaacaca ttaagaagtt
     2761 acgtgacatg cgttcaaaaa gcagaggtaa ccatggaatc atacagcctc acactcgatg
     2821 aggcctgtca gtttcttaag atatccagac caaccgccac caactggata cgaacaggcc
     2881 gcctacaggc aacacgtaaa gatccaacca agccaaaatc tccttacctc acaacacggc
     2941 aagcctgcat tgcggcgctt cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg
     3001 gcataacaga ggaaagaaaa tgtcactctt ccgcagaaat gaaatatggt atgcctcgta
     3061 ttcgctcccg ggcgggaaac gaattaagga atctcttggc acaaaggaca agcggcaagc
     3121 tcaggagttg cacgacaagc gaaaagcaga actctggcga gtagaaaagc taggggattt
     3181 acctgatgtc acttttgaag aggcctgcct aagatggctt gaggaaaaag ctgataaaaa
     3241 atctctcgat tcagataaaa gccggattga gttctggctt gaacattttg agggtataag
     3301 gcttaaagat atctcggagg caaagattta ctctgctgta agcagaatgc ataacagaaa
     3361 gacgaaagaa atatggaaac agaaagttca ggccgccatc aggaaaggta aagaaccgcc
     3421 tgtttatgaa ccaaagccag tatcaactca gacaaaggca aagcatcttg ccatgataaa
     3481 ggccattctc cgtgctgcag aacgcgactg gaagtggctg gaaaaagcgc ctgtcatcaa
     3541 gataccagcg gtcagaaaca agcgagtcag atggctggaa aaggaggaag caaaacgcct
     3601 tattgatgag tgccccgaac cactgaaatc tgtcgtcaag tttgcgctgg caactggtct
     3661 gagaaagtcg aacatcataa atctggaatg gcaacaaatc gacatgcagc gacgagttgc
     3721 ctgggtgaat ccagaagaga gcaaatcaaa ccgcgccatt ggtgtggcgc tgaacgatac
     3781 cgcctgtaaa gtgttgcgtg atcaaatagg caagcatcac aaatgggtgt ttgtacatac
     3841 caaggcggct aagcgagcag atggaacatc aacgcctgcg gtcaggaaga tgcgcatcga
     3901 cagcaagaca tcatggctat cagcttgtcg tcgtgcagga attgaagatt tccgtttcca
     3961 tgacctcaga cacacctggg caagctggct gattcagtca ggcgtcccat tatcagtgct
     4021 tcaggaaatg ggcggatggg agtccataga aatggttcgt aggtatgctc accttgcgcc
     4081 taatcatttg acagagcatg cgaggaaaat agacgacatt tttggtgata atgtcccaaa
     4141 tatgtcccac tctggaatta tggaggatat aaagaaggcg taactgattg aattgtaatg
     4201 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
     4261 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
     4321 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
     4381 ttctgattta tttcgctata gcggcaaaca aacacacacc gctgcgcgtc tgaatcaaga
     4441 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
     4501 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
     4561 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
     4621 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     4681 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     4741 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     4801 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     4861 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata
     4921 taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag
     4981 tgaccatatt acgttatctg gttagcggat tatctaataa agaaattgcc gataagttat
     5041 tacttagcaa taaaacagtt agtgcgcata aatctaatat tta
//
LOCUS       DAZKGS010000242         5008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKGS010000242 DAZKGS010000000
VERSION     DAZKGS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHOQY_004618"
     CDS             <1..261
                     /locus_tag="ACHOQY_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9680566.1"
                     /translation="ENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLI
                     AAGLESNLTVYTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(403..1527)
                     /locus_tag="ACHOQY_004619"
     CDS             complement(403..1527)
                     /locus_tag="ACHOQY_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP9680567.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(2257..2454)
                     /locus_tag="ACHOQY_004620"
     CDS             complement(2257..2454)
                     /locus_tag="ACHOQY_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9680568.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(2520..2790)
                     /locus_tag="ACHOQY_004621"
                     /pseudo
     CDS             complement(2520..2790)
                     /locus_tag="ACHOQY_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3095..3283
                     /locus_tag="ACHOQY_004622"
     CDS             3095..3283
                     /locus_tag="ACHOQY_004622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680569.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            3611..3805
                     /locus_tag="ACHOQY_004623"
     CDS             3611..3805
                     /locus_tag="ACHOQY_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680570.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4148..4276
                     /locus_tag="ACHOQY_004624"
     CDS             4148..4276
                     /locus_tag="ACHOQY_004624"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680571.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(4586..4921)
                     /locus_tag="ACHOQY_004625"
     CDS             complement(4586..4921)
                     /locus_tag="ACHOQY_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680572.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1495 a         1135 c         1035 g         1343 t
ORIGIN      
        1 gagaataata cctcctgggt aagtgagcag atactttcag cgcctccgaa agatgtaaat
       61 attcgacttg gagtcggttc tttagaaggt acaaccgtct cacatgttca gcgattgcat
      121 cattcgttaa tcgcagctgg tttggaaagt aacctcactg tctataccgg tggtcacgat
      181 tatgcctggt ggcgcggagc aattattgat gaattagcaa attataattg caggaaggta
      241 tcaaataata actctgtgta aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt
      301 attatgggag actatctcat ttatttagtg atgtccttca tttatgtgat gaaggacatt
      361 atattactca taacatttct cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt
      421 gttttttaat tttctcatca aaacctctgc aatctctgca ggactgggtt gttcagccat
      481 ttcagcggct acctgatctg aacagaagcg tagtgaatca tcataaagaa ggcgatttac
      541 caaatcactg gtcagtccat gcttgtccgg aataatccca cacccacgct tcgcaacaat
      601 ttctgcatta acagagcgat ctgcaccttc gccaaacaca atctgtggta ttccactata
      661 cagcgctgtc agggtatttc ctgcgccacc gtgatgaata aatccatcag ctccattaag
      721 gaatacaccc ataggtatcc agtcaaccag acgtacattt gatggtagtt ttcgtaaacc
      781 agtacgagca tttattgcaa gttgcaaaat gatatcagca tcaacttcat ttgcagaatc
      841 cataacccat gaaatcagct ccagaccatc aaccattggt tttacagtgc caaggctgat
      901 aagtaaacgt ttccgatcag aatccctgtc ccaccatgtt tcctttacag cacctccgtt
      961 ataaggaata tatctcattg agataaccgg ttctccagca tttttaagaa tgctcatact
     1021 tgggggagca acatcaatcc atgctaaatc acatagtgga ccgctgactc catgccgcct
     1081 gtaagcattg cttaaagaac ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc
     1141 aacagccagc atcactgaag gaattctata tttagcagca accaatgggc ctgccggatc
     1201 aagaggggga tagactatta aatctggcct ccattttcct gcaaaatcga tgaggttatc
     1261 tgccatttca tcgctaaaaa aagagaaatg accaataata ttactttttt tcctcaactc
     1321 ttcctgatgg cgatatccag cctctgaatc taaacctgga actgcatcaa aaacaaccag
     1381 tccagcttct gctgctttat tcgcgaattt gccagcgcta ctaattacta catcatgtcc
     1441 aattacacga aaggcctgag ccagagaaat caatgggtat aacaaaccgt acagcggtgg
     1501 gccaataaaa agaatacgtt ttcgcattaa aaaagtccgt cttctgctta cctgtttatt
     1561 tttctatctc taagaaaaca taattaatat tatttttatg tgagcttatg cacaaaaata
     1621 accaagtata ttatcttctg gtagagattc aggatacagt ttgtaggagc tttctttttt
     1681 gaacaatgat ttgcaggaca gttatgctaa actagcggaa tcagtatcac aacaataaat
     1741 gactgatcat acctgaaatt tgaattaaca ggcatcatga atgtcaatag tctaagcggt
     1801 gactacttat atcgcttgca taaatactaa acactgacta gaaaagtatt ggataacccc
     1861 gtcactaaaa gtaacgcttc ggttatttgc tcggaatgta ttaacgccaa gattgttagc
     1921 cgttacgcct tatatcactg gcgctgacaa atgtttcttt tctaatttac tgaaccggcg
     1981 cagacacgca ggaaagcacg ccggcaccgg tgtaatgcca gttctaatca ataagaatgg
     2041 aatgacgaat cagtcaggaa gggataacaa cccgctgctg aacggaaggt atcagcagga
     2101 agatgaacgg gaatatcgcc agcaccaagg gcagggccgg gttccacaat accagcgctg
     2161 gcattttgca cattgccagc gtaccggcat tacaacacgt ttcatgctgc tgccccccag
     2221 gattcctcga ttcgttgcta catccactgc tctacttcac ttccactgct ccacttcact
     2281 tttgtaccag cggctgctgc gccccagttt gatgggttta gggaaatggc cttcactgat
     2341 caacttgtaa aaccatttgt cggtcatacc ggtgaaactg gtgatatagt tcatgtcgat
     2401 cagggaatta tcggcacgca gaaatacgct gtcgtggctt gttgtagtca acatggtcag
     2461 aatgtcctgt gagatttatg aagattgtgc gaatgcggga aatctactga gctgtgcttt
     2521 cagaactggc ctgttacggg ataccaggga ttaccggcgg ggtaacgggc ttccggatca
     2581 tacacaccac gattatcgcg gacaaaatca ctgaacgccc atatcacctc tttaagtatg
     2641 tcttcgcagt ccggtaaatg acgatccagc gccacatccc gggtggtaca gctttgatgc
     2701 gcccggtgac acaaagcccg gattgttcca gacatcctga atcaaacgcc ccagattagg
     2761 ggcgtcgaaa tatgcctctc tgaccattat attccggcgt ataggtagca ggtctgaagt
     2821 gacaatgcgt cacctgacgt taaaagtcac ccaagatgac cccagtgggg gcaacaagta
     2881 taaaaccgtt ttttgattaa gtggtcggat atgaaaaggg gaaggtttat gtgaaaaata
     2941 cctgtgtttt ctttgaaaac acacaacagt atcaggttat ttatgtttat acagtgcgtt
     3001 ccaaactgac ctgaataatt acgcaagttg caacaataaa acatttttgc cgtagtgtga
     3061 ggtactccgg taaattgctt cccaggtgca gacaatgact aacgaagaac tacaggcaat
     3121 cgcattatcc atgcttgagc gccagcgcga aaaagtacac cagcaacgag agtctttgct
     3181 taacccatct ccattcacca ggccaaaacg attggagctt gaagcattct tggacaaata
     3241 cccacggcgg cttaaatcag gcaaaaccag accaccaggc tgatagctca cagtttgttc
     3301 tccgattcaa taaaacggat aagaaccatc agcgcggtag cgtaaggcac tacagcgcat
     3361 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
     3421 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
     3481 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
     3541 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
     3601 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
     3661 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
     3721 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
     3781 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
     3841 gcatcgttat cagcgcactt gattttcttt atatccactg acgagtttgc cggtcattct
     3901 ggtatcatgc agatatttac ggcgtacaag cacactagca tgacattgta ataaggcctg
     3961 agcctaacgc tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca
     4021 tgtaatcgta agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg
     4081 gtgtccacca aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca
     4141 acgaaaaatg ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg
     4201 ccagttctcc gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt
     4261 gatggagcat cgttaatacg atgctatacg atgcattcac agtgcccggc cagaggatgc
     4321 cccgtcgctg catatggatc agggttggca atatcgaatt gcaggctata ggcaaagtta
     4381 aagccccatg gagtagcaca aaatatgccg cacaaaggaa acggtctgaa taacgcagtg
     4441 atgaagaact tcttcagcac actgctaaaa cgcatagtga tcgagcgctg attctggcga
     4501 acaactgaac taatacatca gaatctgcat tatgttaaat aaatataaaa agatggttta
     4561 aataccccgt tacttttgac ttacactata cggtatcgca tcgtttaata ttcgcaccgg
     4621 ccagattttt atttctatta gttgtcacaa tactgaatgc gtacgaccac agtattctgg
     4681 ctcctgtgtg gttatgcttt aattctgcgt tccgggcaga taagcagttg cttgcaggaa
     4741 tccttcttgt gttaatgtca gttccccttt taccagtgct gatttccaca ttccgtccaa
     4801 cagagcttat agcctttccc tggattatag cattgtccgg ctgaagttct ttttgaataa
     4861 taatagaagc actgctggca gatccagtcc gtttttcata acccactgta ctgataacca
     4921 taatctaatc agtagaaatt gagtcgaaaa taagcactac tccatacagg ataattagag
     4981 gtcagtttga ttattcacaa ttcatcat
//
LOCUS       DAZKGS010000243         4991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKGS010000243 DAZKGS010000000
VERSION     DAZKGS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..169
                     /locus_tag="ACHOQY_004626"
     CDS             <1..169
                     /locus_tag="ACHOQY_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9680573.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            612..4514
                     /gene="espP"
                     /locus_tag="ACHOQY_004627"
     CDS             612..4514
                     /gene="espP"
                     /locus_tag="ACHOQY_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFP9680574.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4762..>4991)
                     /locus_tag="ACHOQY_004628"
     CDS             complement(4762..>4991)
                     /locus_tag="ACHOQY_004628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680575.1"
                     /translation="AQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLFF
                     VDKEAGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1514 a         1134 c         1113 g         1230 t
ORIGIN      
        1 ccatggtggt gagcggggag cgctactgta cagcctgatc gggacgtgca aactgaatga
       61 cgtggatcca gaaagctacc ttcgccatgt gcctggcgtc atagcagact ggccggtcaa
      121 ccgggtcagc gaactgcttc cgtggcgcat agcactgcca gctgaataac acatccccgt
      181 caatacggcc ctcgctgtac gcttacgcat aaatattgaa tacatttact gagcatgcaa
      241 aagaaaaaga caaactaatc ccggcctttc acagaatttc ctctatcaga ggctgagttg
      301 acaacctgct cacccaggaa atagtaacac ccgaaaacat gaagagactc tgataaacaa
      361 tctcgataca tattcctcat ctttcacagc atctcacttc tttaacagga attattatta
      421 tctacgcctc ctcctttcat gtatccctca tgaatgatta agataaacat actgtgtttg
      481 ttatccgtat gataacaaac acataaagga gtaaacattt atctggatga tgattacaat
      541 gttcacgact gtcagagaca cacctctgat acattagaaa aaccaatgtt tcccttaaaa
      601 atggagctta tatgaataaa atatactctc ttaaatacag ccatattaca ggagggttaa
      661 tcgctgtttc tgaattatcc ggcagagtat catcaagagc aactggtaag aaaaaacaca
      721 aacgcatact tgcattatgt tttttaggct tattacaatc ctcatattct tttgcgtcac
      781 agatggatat ttcaaatttc tacatccgtg actatatgga ttttgcacag aacaagggca
      841 tatttcaggc tggcgcaaca aatattgaaa tagtgaagaa agatggctcc accctgaaac
      901 taccggaagt accatttcct gacttctcac cggttgcaaa caaagggtca accacatcta
      961 ttggtggtgc atacagtatc acagccacac acaatacgaa aaaccaccac tcagttgcga
     1021 cgcaaaactg ggggaacagc acgtacaaac aaactgactg gaatacttca catcctgatt
     1081 ttgcagtatc ccgacttgac aagtttgttg ttgagacccg aggtgcgact gaaggcgcag
     1141 atatttcgtt atcaaaacag caggcacttg aacgttacgg ggttaattat aaaggagaaa
     1201 agaaacttat cgcattcaga gccggctctg gtgtggtatc cgttaaaaaa aatggacgca
     1261 taactccatt taatgaggtt tcttataagc cagaaatgtt aaatggctct ttcgttcaca
     1321 ttgatgactg gagtggatgg ctgatattaa ccaacaacca gtttgatgag tttaataaca
     1381 ttgcctctca gggtgacagc ggttcagcac tgttcgtcta tgataaccaa aagaaaaagt
     1441 gggttgtcgc tggaactgtc tgggggattt ataattacgc caatggcaaa aaccacgcag
     1501 catacagtaa atggaaccag acaaccattg acaacctgaa gaacaagtat tcttacaacg
     1561 tggatatgtc aggagctcag gttgcaacca ttgaaaatgg aaaactgaca ggcactggct
     1621 cagacaccac cgatataaaa aataaggact taatatttac tggcggtgga gatatcctcc
     1681 tgaaatcctc ttttgataat ggtgctggcg gtcttgtctt taatgataaa aagacctatc
     1741 gagtaaacgg ggatgatttc acctttaaag gtgccggtgt tgatacaaga aacggcagca
     1801 ccgttgagtg gaatatccgg tatgataata aagacaacct tcacaaaatt ggtgatggca
     1861 cattagatgt ccgaaaaacc cagaacacca acctgaaaac aggtgagggt cttgtcattc
     1921 ttggagctga aaaaacattc aataatatct acataaccag tggtgatgga actgtccgac
     1981 tgaatgcaga aaatgcactg tctggcggtg aatacaacgg tattttcttt gcgaaaaatg
     2041 gcggaactct tgacctgaac ggatataatc agtctttcaa taaaattgct gcaactgatt
     2101 caggtgctgt aataaccaat acgtcaacca aaaaatccat tttatccctg aataatactg
     2161 ctgactatat ctatcacggc aacataaacg ggaatctgga cgtacttcag catcatgaga
     2221 cgaaaaaaga gaaccgtcgt cttattcttg atgggggcgt ggacacaaca aatgatataa
     2281 gcctgcgtaa tacacaactg tccatgcagg gacatgccac tgaacatgcc atttatcggg
     2341 atggagcttt ctcttgttca ctaccagctc ctatgcgctt tttgtgtggc agtgattatg
     2401 ttgcaggaat gcaaaataca gaagctgatg ctgtaaaaca aaacggaaat gcctataaaa
     2461 ccaacaatgc tgtctctgat ttatcgcagc cagactggga aaccggaaca ttcagatttg
     2521 gaacgctaca tcttgaaaat tccgattttt ctgttggtcg taatgcaaat gtaatcgggg
     2581 acattcaggc cagtaaatca aacattacta ttggtgacac tacagcatat attgatttgc
     2641 atgctggtaa aaatattacc ggtgatggtt ttggcttccg ccagaatatt gtgcgtggaa
     2701 actcacaagg agaaacgctg tttacaggag ggatcacagc agaagacagc actatcgtta
     2761 ttaaagataa agcaaaagca ttattttcaa attatgtata cctgctgaac acaaaagcaa
     2821 ccatagagaa cggtgctgat gtgacaactc aaagtggtat gttctccacg agcgatatca
     2881 gcatctctgg taatctgtcc atgacaggca atcccgacaa agacaataaa ttcgagccct
     2941 caatatatct gaatgatgct tcttatctac tgactgacga ctccgccaga ctcgttgcca
     3001 aaaataaagc atctgtggtg ggagatatac actccactaa aagtgcatcc atcatgtttg
     3061 gtcatgatga aagcgacctc tcgcagttgt ctgacagaac ctcaaaaggg cttgcacttg
     3121 gtcttttagg tggctttgat gtctcatatc gcggttcagt caatgccccg tcagcatctg
     3181 ccactatgaa caatacctgg tggcaactaa ccggagattc tgcgctgaaa acactgaaaa
     3241 gtacaaacag catggtctat ttcactgaca gcgcaaacaa taagaaattc catacgctga
     3301 cggtcgatga gctggcaacc agcaacagcg cctatgcgat gcgtacaaac ctttctgaat
     3361 cagacaaact ggaggtcaaa aaacacctgt ctggtgagaa caatatttta ctcgttgatt
     3421 tccttcagaa accaacgcct gaaaaacaac tgaatattga actggtaagc gcgccaaaag
     3481 acaccaatga aaatgtcttt aaagccagta aacaaaccat tggtttcagt gatgtaacgc
     3541 cggtcattac aaccagggaa accgatgaca aaataacatg gtcactgaca ggctataaca
     3601 cggtagcaaa caaggaagca acccggaatg ccgccgccct gttctctgtt gactataaag
     3661 cgtttctgaa cgaggtcaac aacctgaaca aacgtatggg tgacctgcgt gatatcaacg
     3721 gcgaagccgg tgcatgggca cgcatcatga gcggtaccgg ctctgccagt ggtggtttca
     3781 gtgacaacta cacgcacgtt caggtcgggg tcgacaaaaa acacgagctg gacggactgg
     3841 atttgtttac cggtttcact gtcacacaca ctgacagcag tgcctccgcc gatgttttca
     3901 gtggtaaaac gaagtctgtg ggggctggcc tgtatgcttc cgccatgttt gattccggtg
     3961 cctatatcga cctgattggc aagtatgttc accatgataa tgagtacact gcaacctttg
     4021 ccggactcgg aacccgtgat tacagcacgc attcatggta tgccggtgca gaagcgggct
     4081 accgctatca tgtcactgag gatgcctgga ttgagccaca ggctgagctg gtttacggtt
     4141 ctgtatccgg taaacagttt gcatggaagg accagggaat gcatctgtcc atgaaggaca
     4201 aggactacaa tccgctgatt ggccgaacgg gtgtggatgt gggtaaatcc ttctctggta
     4261 aggactggaa agtgacagcc cgtgccggtc tgggctacca gttcgacctg ctggctaacg
     4321 gcgaaaccgt attgcgggat gcatctggtg aaaaacgcat caaaggtgaa aaggacagcc
     4381 gtatgctgat gtccgttggc ctgaatgcag aaatcaggga taacgtccgc tttggactgg
     4441 agtttgagaa atccgccttt ggtaagtaca acgttgataa tgcagtcaac gctaatttcc
     4501 gttactcgtt ctgatactcc gtattcaaaa gggcctgcgg gccctttacg ctcagtgcct
     4561 gccgccctga tatgtctgag agacatttcc gggatgtcag tcgactggtt tacttaactg
     4621 cgatgactaa agctggcagt aatgccggct tttttacggc cacatacgtc ttcctctcat
     4681 ccggaactgt gatacaaatc ctgcatacag gattgaccgc cggttcctgc aaatggcaca
     4741 ttttcagtaa gatagcgaag gttacattcc cccgtcatgt gtggcacaag ctgtcaccgg
     4801 gaaagaaacg gaaagcacat gaaaaacatc aaaactcaca ccggcctcct tatccacaaa
     4861 gaacaaacgc ggcgcgttcg tcttcatgaa acacccactg cctggtgcca cacgcaccgg
     4921 gaatgctaca gcaaaacgac cggtcgccgc tgcggcagcc ctgacagtct cagcaggctg
     4981 attctgagca g
//
LOCUS       DAZKGS010000244         4964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKGS010000244 DAZKGS010000000
VERSION     DAZKGS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..412)
                     /locus_tag="ACHOQY_004629"
     CDS             complement(<1..412)
                     /locus_tag="ACHOQY_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9680576.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVV"
     gene            complement(412..741)
                     /locus_tag="ACHOQY_004630"
     CDS             complement(412..741)
                     /locus_tag="ACHOQY_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680577.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(738..3350)
                     /locus_tag="ACHOQY_004631"
     CDS             complement(738..3350)
                     /locus_tag="ACHOQY_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9680578.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSSKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAA
                     LAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            complement(3331..3744)
                     /locus_tag="ACHOQY_004632"
     CDS             complement(3331..3744)
                     /locus_tag="ACHOQY_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9680579.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGIYGGVRYVPASG"
     gene            complement(3771..4193)
                     /locus_tag="ACHOQY_004633"
     CDS             complement(3771..4193)
                     /locus_tag="ACHOQY_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9680580.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(4207..4959)
                     /locus_tag="ACHOQY_004634"
     CDS             complement(4207..4959)
                     /locus_tag="ACHOQY_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680581.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTGFTLAWKPGEEGQKWLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
BASE COUNT          981 a         1671 c         1145 g         1167 t
ORIGIN      
        1 ccaccaccca ccggtcagtc agctcctgct ccgggtcggc ctccggattg cctgccacaa
       61 agttcaccgc atccagaaaa cgcgcataca cccggcggcg gaccaccgtg gcacccacca
      121 ggctctgcaa atcctccgcc atcccggtga caagaccgaa cagattggac accgtcagcg
      181 acgggcgggc actgctgccc tttccgttca tctcaaagcc gctgccctca atcgggtacg
      241 cctgatattc acgcccctgc caggtcaccg gctccccttt ttcattcagc tcattgcaga
      301 aaaaataccg ctcaccgccc tgcgccgtca ggtcgatttc ccagagtacc acccgcggtg
      361 actgctctga tttaaccgac tcgttcagac tttcttcgtg aatatcctgc atcagttcac
      421 cacctgctta aactccgcgc tgaactcaac gcgcaacatc ccgacccgcg cagaccaccc
      481 ggcacaggtc acctttatct gccggtatgc atagggtggc ttccacaaaa atgccttcca
      541 gcccccgtgc tctgccagaa acgcttccag atgccgggcc tcctcccggg tcacggaaag
      601 cgtcacccgg tatgttttca ggtcagcatt cagccctgcc gccatacgct gtgagtaccc
      661 gtcaccaaaa cgcacttcac gcaccgatgg ctgtgagttc acctccatat ccggcttcac
      721 tttccagcga aaggttttca tcgcccgctc cctgataaca taccgccatc acgcaactgc
      781 agccggagct catcctgcgc ccccttgcgg gccatgtcat acaccgcctt catcagctgc
      841 ggccccgcct gtccgttggt gccgtcgttc tgaatcacca cgtgattgtt ctgattaaaa
      901 ttaatgcctt ccgtccgccg catctgcgcc ggacttccgg caccgcccac ataaccacct
      961 tccgcatagc cgcgcatcag acggtaaaga ttccccacac ctatccggct ggttgcctct
     1021 ttcgtgaaaa caaactcccc gcggtgaact atccccgcag gctcatattt gccgcccgtc
     1081 cccgtaaatc ctccggtcgc gaaatggaag ttcgccgccg cagcctcaat ggccgtcccc
     1141 gaggaagcag atgcaccacc accgaaagca ccgccaatgg cgctgccgat acgcccgaca
     1201 atgcccacca tggcctgttt aagcaggatt tctgtcatca tggacagcac agaacgggtg
     1261 aatccccgcc agtctgcctc tgcaccggtc agcatcgccg ccatattctg tgcaataccg
     1321 tcaaaggtct gcgtggcagc acttttaacc tgcgaaaaac tgtccgtcgc actttccgcc
     1381 cactcgcccc agccggactt cagcccggcc atccagcttc cacgaagctg ctcctccgca
     1441 gaccaggtgt tcttcagtgc cgatgtggcc ttcgccagcg cagccggatt atcaccgtac
     1501 acgtcccgaa gacgctgcgc ttcagactcc cgctgcgcct gacggtcagt gagaccgcgg
     1561 gcttttgcgc tgatggcggc ctgcttcgag ctctgctgct cttcaaaccg caccgcctgc
     1621 tgtgccagct cattcaggcg tttctggtat tcaaccttgt cgcccagctc agccagctgg
     1681 cgtttgtact ccagcgtctc gtctttatgc gccagcaggg atttttcctg ctcagataac
     1741 tgtcgtttcg tggcagcctc tttcaggacc gcatactgac tttccgcctt ccataaatca
     1801 cggcgctgcc ggctgatttt ctcattcgca ccgctgtgtt tttccagcgt cctgagctca
     1861 gcttcaaggg caagcagagc ctctcttgcc tgctcctctt ccctctcccc ggcagagcgc
     1921 gttttcggtg atgtatgctt tttacctgtc agctcttcag ccagacggct gacggcttcc
     1981 tgctgccccg gacctttgct gacgcctgtt gcacgcgaac ggttgatgta ccccatttcc
     2041 ccctggcgta tacgcgcatc ccgttccgca atggattttc tcagcgccag ttcatcgcgt
     2101 tttgttttct caataaatac gcggttctct tctgccagtt caccaaacaa cgcaccaacg
     2161 ccgggcacat tctttgtcgt ttcccaggct gactgaataa attcagccag cgccagatcc
     2221 ccctgcacaa gcagcagctt cacttgttca acggttccgg ccaccacgtc agtgatcaga
     2281 ctgagtgccc ccagtgtatg atcacctatc catgcccatg cgtcagaagt ccaggtttta
     2341 acatcgtccc agattttttc caccggcgtg gccgctttat caagttgctc cagacgtgca
     2401 ttcatgacat ccgcaaacag ggacatcgcc tccgtcaccg cagcctgttt acctttcgtg
     2461 cgctcaagct catcaatatg gcgtaactgg gaaacgctca ggaagttata ctgctgattc
     2521 agggaggcca gcgccttcac cggatctgct gcaatccctt caaaggcttt ttccaccttc
     2581 ccggcatcgt cccccaccgt ctgcagccat ctctgagagg tttcccccat gatccgtagc
     2641 tgcccggcgg tatatttccc gctttctgcc agacgggcca gattttctgc cgcctgtctg
     2701 ataccaccac cggcttcatc gctgatcacc ccggccattt tccacaattc tgccgttgtg
     2761 gtggcagccg cccctccggt caggatcagt gaacgcaata aggcccggtc agcctgctct
     2821 gcctgccagg cggcggcagc aagcgcggcc agtacggcaa ccccgccacc tgccgccaca
     2881 cgggccaccg acataaatcg tcccagctca ccggcattcc gggcattttc agccagtgca
     2941 tttgccgtat ctgacagcga ctcctctgat gattcagagg catccctgat cccgagaagt
     3001 tcctccttca gcagggtaag caggctgagc ggtccaccga atgaatcgct gatctgcccc
     3061 ccctgctgca gcatgataag gaagggattc tgaccaccgg caagctgagt gacaatatcc
     3121 gtgaactgtg cgggcagtgt gcgcatggca gccttatact gtccgactga tatcccggct
     3181 ttttgtgccg ccagcgcctg tcggctcagc ccctgttcaa cagtactggc ggtttttctg
     3241 gcatccgcct ccagactgga aaaatgacgg cgtacccggg ccatctgttc atcaaaccgg
     3301 gccgcatcca gactcaaatc aataaccaga tcacccgctg gctgggacat atctcacacc
     3361 tccgtaaatc cccgctgaag ccatcattaa tgcaacatca tcctcgctga catccaccac
     3421 atccgcagaa ggtgaaatat cgcgccctcc gtcccctccg aaccggacgc ctccggcaac
     3481 tcctgtcgct ttctgcatca gcatttcttc ctcgtccggc atctccgtct gctcttcctc
     3541 acacgctgga gcaagcagac tgaaatccgc cggatgcata tccggatcgc caaaaaacag
     3601 gctgagtacg gcgtacatca gcccggaaaa atgagcgtcc agttgggtat cctgaaaata
     3661 atgcgtgcag taaaaacgtc gccagtcggc atattcggtg gatgtcatcc cggcaagcat
     3721 ggcgcgccag tcgggtctcc ccatctctcg cgccagtttc aggacaaagt tcagctcgcc
     3781 ttcgaatgct tttttgatgt taccggctca gtcgcttctg ctttcccggt ttgttcagga
     3841 tcggcatcgt gccggttatc cagcatacct gaaagataaa gcacccggtt cgttgcctga
     3901 ttcagtgcat cagcaggcca tcccagcatc acttcacggc ggatctgctg catctctgtc
     3961 tccggagagg ccagagtgcc tttgagggaa tgggaatgcc atagcgacat cgccacaagc
     4021 agggatgccg tttccagata tcgctggtta atgtgcacga catcatgctt cgttgtctcc
     4081 tgttgttctg cgtctgaaac aaactttata taatcaaacc gctgcagcgc agacaattcg
     4141 gaaagcgtga cggacacacc gttatattca aattgttctg ttttcagaaa catgtattac
     4201 ctccgtttac cctgcagcgc ccgcttcagt aacggtgact tcagccactg cggcgaactg
     4261 accatttccg ctcaccacag ggatctgcac cttacctgtc gccacgccgt ttaccgtaat
     4321 tgtcatatct ttcacactaa tggtggcttt cgacggatcg gcggaaaccg ctctgaacgt
     4381 cttgtcggtt gcactttccg gctcaaaaga aaccgtcagg gtggttgttt tcccttttgc
     4441 caccgtaccg gatgtcggcg tcaccttaat cgcagtgacc ggcgtaattt tgctgcgttc
     4501 ttccgctaca gaaggtttgc ccacgttggt cactttcacc gtgcgggtga tcacttcttt
     4561 cgccgtcacg gccttaccga tactgctgac ccagccacgg aacacatcca ccgtgccatt
     4621 tgggaaacgg attttatagg cccgcacatc cccgctttca aaccagccta taagccattt
     4681 ctgaccttct tctcccggtt tccaggccag cgtaaaaccg gtatctcctg cagacttctg
     4741 cccctgcccg gtcgcggtcc agtccgcgtc ttcatcatcc aggtagttat catcgtaggg
     4801 ttctgccgtc atctcgcccg gcgtcagatc cttcacctta gccagtcgct gccagtcatc
     4861 gtctgacaac gggtttgcat aagcatcacc cttgccgttg taaacccaca gagtggtacc
     4921 ggcacctttt accggctcaa ggggatttgg tgttgccata tcgt
//
LOCUS       DAZKGS010000245         4814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKGS010000245 DAZKGS010000000
VERSION     DAZKGS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHOQY_004635"
     CDS             complement(<1..596)
                     /locus_tag="ACHOQY_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9680582.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHOQY_004636"
     tRNA            complement(711..786)
                     /locus_tag="ACHOQY_004636"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHOQY_004637"
     tRNA            complement(793..867)
                     /locus_tag="ACHOQY_004637"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(983..1067)
                     /locus_tag="ACHOQY_004638"
     tRNA            complement(983..1067)
                     /locus_tag="ACHOQY_004638"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Tyr,seq:gta)
     gene            complement(1076..1151)
                     /locus_tag="ACHOQY_004639"
     tRNA            complement(1076..1151)
                     /locus_tag="ACHOQY_004639"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1116..1118),aa:Thr,seq:tgt)
     gene            1513..2463
                     /gene="coaA"
                     /locus_tag="ACHOQY_004640"
     CDS             1513..2463
                     /gene="coaA"
                     /locus_tag="ACHOQY_004640"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9680583.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2492..3457)
                     /gene="birA"
                     /locus_tag="ACHOQY_004641"
     CDS             complement(2492..3457)
                     /gene="birA"
                     /locus_tag="ACHOQY_004641"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9680584.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3454..4482)
                     /gene="murB"
                     /locus_tag="ACHOQY_004642"
     CDS             complement(3454..4482)
                     /gene="murB"
                     /locus_tag="ACHOQY_004642"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9680585.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1216 a         1228 c         1095 g         1275 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtgccgaa attggtggtg ggggaaggat tcgaaccttc gaagtctgtg
     1021 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccgga cttgatggtg
     1081 ccgactaccg gaatcgaact ggtgacctac tgattacaag tcagttgctc tacctactga
     1141 gctaagtcgg catcaagtag cgcgcattct atggagacat gcgagttcat gcaactaaaa
     1201 aattgcataa tttgttttat tggtcacatt ttatgcgaca cgatgaagaa acagccgaac
     1261 ggagggcaga ttagcacact ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa
     1321 ccataattct ttttatcaga tggaatatct gtcacattgc ttttcaacga tagcttcctg
     1381 gcagagattt tttcttatta ttcctcccca tctggtgtta ccctcctgcc cattaaccca
     1441 ttcaacagaa ctgtgacgcg ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc
     1501 agaaacatgc ttatgagtat aaaagagcaa acgttaatga cgccttacct acagtttgac
     1561 cgcaaccagt gggcagctct gcgtgattcc gtacctatga cgttatcgga agatgagatc
     1621 gcccgtctca aaggtattaa tgaagatctc tcgttagaag aagttgccga gatctattta
     1681 cctttgtcac gtttgctgaa cttctatata agctcgaatc tgcgccgtca ggcagttctg
     1741 gaacagtttc ttggtactaa cgggcaacgc attccttaca ttatcagtat tgctggcagt
     1801 gtcgcggtgg ggaaaagcac aaccgcccgt gtattgcagg cgctattaag ccgttggccg
     1861 gaacatcgtc gtgttgaact gatcactaca gatggcttcc ttcaccctaa tcaggttctg
     1921 aaagaacgtg gtctgatgaa gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg
     1981 gtgaagtttg tttccgatct caaatccggc gtgccaaacg ttacagcccc tgtttactcg
     2041 catcttattt atgatgtgat cccggatgga gataaaacgg ttgttcagcc tgatatttta
     2101 attcttgaag ggttaaatgt cttacagagc gggatggatt atccacacga tccacatcat
     2161 gtatttgttt ctgattttgt cgatttttcg atatatgttg atgcaccgga agacttactt
     2221 cagacgtggt atatcaaccg ttttctgaaa ttccgcgaag gggcttttac cgacccggat
     2281 tcctattttc ataactacgc gaaattaact aaagaagaag cgattaagac tgccatgaca
     2341 ttgtggaaag agatcaactg gctgaactta aagcaaaata ttctacctac tcgtgagcgc
     2401 gccagtttaa tcctgacgaa aagtgctaat catgcggtag aagaggtcag actacgcaaa
     2461 taatttgcag gggagcgaat actccccttt cttatttttc tgcactacgc agggatattt
     2521 caccgcccat ccagggtttt attattccat cctgctcaag taataaagcc ccctgtttat
     2581 ctattccgcg tgaaatgcca aatatttctt tatcaccaat gataagtttc actgggcgat
     2641 taataaaatt atccagcttt tcccagcgcg acagataagg tgccaatcct tcttgttcga
     2701 agagttccaa cgcagcacgt aattcacgta ttagcatggc cgccaacgta ttacgatcga
     2761 gattgatccc cgcttcctgc agcgtgatcc acccctgatt aacgacactc tcttcaacac
     2821 ggcgcattgc catgttgatc ccggctccaa tgactatttg cgccgcatcg ccagttttgc
     2881 cagtcagctc cacaagaatg cctgccagct tgcgatcctg cagatagagg tcattaggcc
     2941 atttgacacg aactttatct gctcccagct tgcgtaatac ttccgccatc acgataccga
     3001 taaccagact taaaccaatc gccgccgccg ggccttgttc cagacgccag aacatcgaca
     3061 aatataagtt tgcgccaaaa ggcgaaaacc atttccgccc ccggcgacca cggccagcct
     3121 gctggtattc tgcaacacag gcatcgcccg atttaagctc tccgatacga tcaagaaggt
     3181 actgattcgt ggagtcaata actggcagca cggctacact accgccatcc agctgaccca
     3241 atatctgttc agcattaagt aactggatgg gctcaggcag gctgtatcct ttacccggaa
     3301 cggtaaagac atcaacgccc cagtcacgca gtgtctgaat gtgtttatta atagccgccc
     3361 ggctcattcc cagcgtttca cccaactgct cgccagagtg aaattcaccg ttcgctaaca
     3421 gggcaatcaa tttcagtggc acggtgttat ccttcatgaa attgtctcca ctgcgctcac
     3481 ttcacctgat gcaccaataa agcggacttc aggctcaagc cagacattaa atttttcacc
     3541 cactttttgt cttacatggt gtgccagttg caccacatct tcgctttttg cattgtcttc
     3601 attaatgaga actaacgcct gttgacggtg caccgcagcc ccacccattt gcatcccttt
     3661 tagctggcac tgatcaataa gccaacctgc tgccagtttt actgaaccac ccgcctgggg
     3721 gtaatttggt gctgttggaa attgcgccag taatgcttta gccgtttcgg cagatacaac
     3781 agggtttttg aagaaactac cggcattgcc attcactttt ggatcaggga gtttggtggt
     3841 gcgcatatga cacaccgcat taaatacttg ctgtggcgtt actgttgtag gatccagacg
     3901 agttaagtca ccatacgtta gtacaggttg ccactctttt ggcagacgca gacctacggc
     3961 tacaatggcg aagcgatcct ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg
     4021 gcactctttg gcagttaagc gcacttgctt gcctgtcgcc agttcaacac aatcaacata
     4081 agcgcaaact cgctgtaatt ctacgccata agcaccaata ttctggatag gtgatgagcc
     4141 gacacaacca ggaattaatg ccagattttc cagaccaggc ataccttcct gcaaagtgta
     4201 ttttaccagg cgatgccagt tttctccggc tcctacatgt aaataccacg catcaggttc
     4261 atcatgaatt tcgatacctt tgatccggtt gatgatcacc gtgccgcgat agtcttccag
     4321 aaaaagtaca ttacttcctt cacccagaat aagaacgggt tgtccttctg cggttgcata
     4381 ctgccaggca ttgagtaatt gttgttcgtc ttcggcacat acaatgtgct gagcattatg
     4441 atcaatgcca aatgtgttcc agggttttaa ggagtggttc atagctgctt tcctgatgca
     4501 aaaacgaggc tagtttaccg tatctgtggg gggatggctt gtagatatga cgacaggaag
     4561 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct
     4621 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa
     4681 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4741 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4801 ctctcgcatg ggga
//
LOCUS       DAZKGS010000246         4762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKGS010000246 DAZKGS010000000
VERSION     DAZKGS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..823
                     /gene="ydiV"
                     /locus_tag="ACHOQY_004643"
     CDS             110..823
                     /gene="ydiV"
                     /locus_tag="ACHOQY_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9680586.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            886..2322
                     /gene="selO"
                     /locus_tag="ACHOQY_004644"
     CDS             886..2322
                     /gene="selO"
                     /locus_tag="ACHOQY_004644"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9680587.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(2326..2517)
                     /gene="hemP"
                     /locus_tag="ACHOQY_004645"
     CDS             complement(2326..2517)
                     /gene="hemP"
                     /locus_tag="ACHOQY_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9680588.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2649..3695)
                     /gene="aroH"
                     /locus_tag="ACHOQY_004646"
     CDS             complement(2649..3695)
                     /gene="aroH"
                     /locus_tag="ACHOQY_004646"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9680589.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3852..4685)
                     /gene="ppsR"
                     /locus_tag="ACHOQY_004647"
     CDS             complement(3852..4685)
                     /gene="ppsR"
                     /locus_tag="ACHOQY_004647"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9680590.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
BASE COUNT         1174 a         1127 c         1226 g         1235 t
ORIGIN      
        1 tggttaacat ggaacaaatg aaagttactc cttatgtaat tgttatgtct tatggcctca
       61 tcccttttag ccggatactg aaaaacatcc ttcgagaggg acggttacca tgaagatttt
      121 tttggagaat ctttatcatt ctgattgcta tttcctgccg ataagagata accagcaaga
      181 ccttgtcggc gttgagttaa ttacccattt ctccagtgaa gatggtacgg ttcgtatccc
      241 gacgagtcgg gttatagcgc aacttaccga ggagcaacat tggcaattat tttcagagca
      301 gttggaatta ctaaaatcat gccaacattt ttttattcaa cataaactct ttgcctggct
      361 taatttaacg ccacaagtgg ctacattatt attagagagg gataattacg caggcgaact
      421 attaaaatat ccgtttattg aactgttgat taatgagaat tacccgcatc tcaatgaagg
      481 aaaagataat cgcgatcttt tatccttatc gcaaatgtat ccccttgtgt taggcaattt
      541 gggagcaggt aatagcacaa tgaaagcggt ttttgatggt cttttcaccc gcgtcatgct
      601 ggataaaagt tttattcagc aacaaatcac gcatcgctcc ttcgaaccgt ttattcgtgc
      661 gatccaggcg caaatttccc cttgttgcaa ttgcatcatc gcggggggca ttgatacgac
      721 agaaatattg gcacagatta cccccttcga tttccatgct ctccagggat gcctgtggcc
      781 tgccgttcct ataaatcaga taacgacgtt ggttcagcga taaccctcct gtttgctggt
      841 gtttaagacg agagtaaccg tctacactat taaacaggag gatctatgac cctgtctttt
      901 attacccgct ggcgcgatga attgccagca acctatacag cactttcccc tacgccttta
      961 aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag tattccatcg
     1021 tcgctgttta aaaatggcgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca
     1081 ccactggcgc aggtttacag tggtcatcag ttcggcgtct gggcgggcca actgggtgat
     1141 gggcgcggta ttttactcgg cgaacaactg cttgctgatg gcactacaat ggactggcat
     1201 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
     1261 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
     1321 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt ggagccaggc
     1381 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
     1441 tatcgccgcg agccggaaaa ggttcgtcag ttagctgact ttgccattcg tcattactgg
     1501 tcatatcttg aagatgatga ggacaaatac cgcctctggt ttagcgatgt tgtcgcacgt
     1561 accgcatcgt taattgccca atggcagacg gtcggctttg ctcatggggt gatgaatacc
     1621 gataacatgt cgctgctggg gctgacgctt gattacgggc cgtttggttt tctggatgat
     1681 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag ctttgataat
     1741 caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc accatttgtt
     1801 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
     1861 ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
     1921 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
     1981 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgatga gtttattgat
     2041 cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt
     2101 agcgatagtg agcgtcagca gctgatgcaa agcgttaacc cagctctggt gttgcgcaac
     2161 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgacgga attgcaccgc
     2221 ctgcatgagg cgttgcgaaa tcctttcagc gacagagctg atgactatgt cagtcgtcca
     2281 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tggtcaacag
     2341 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
     2401 tttgccatcc ggacctaaca gtgtctggct ggaaattcgc cgaataggct gcggggaagc
     2461 ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat aacgcataaa
     2521 aattaataat aattgatata acaatgattc ttattatcaa tattatggct gaccagagca
     2581 agcagtaatg tgctgagaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
     2641 gcacgcattc agaagcgggt atctaccgca gaggcaagtt tttcgaccag gcgttcggta
     2701 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
     2761 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatcgccgta
     2821 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
     2881 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc
     2941 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
     3001 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
     3061 tctggcgaga gaaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
     3121 gagttgccat cggtaccatt tttaaaacct accggacagg agagtgccga agccatttca
     3181 cggtggatct gactttcggt agtacgtgcg ccaatcgcgc cccaactgat taaatcagca
     3241 ataaactgac cggtcaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
     3301 acctgtaaaa gtaatttgcg cgccatctcc agaccgtgat ttacccgata gctgccgttt
     3361 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
     3421 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
     3481 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt
     3541 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
     3601 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
     3661 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
     3721 ctacggcagg actaccggga agtgatgcgc atcacgataa accaatcgca gctgatttca
     3781 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa ataagctaat
     3841 gcactagttc tctagtacat tcggcggcta agccccatga tatcgaggag cttggtggca
     3901 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaac
     3961 gcttctactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg gctgttctcc
     4021 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
     4081 tgctgaagcg gtttgagcga cgcgggtagc accagattgt ccatatcgtc ggcaataaag
     4141 gggtagtttg ccgcgcggat accaaactgc attgccagat acagactggt gggggtttta
     4201 ccacagcgag aaacaccgag caggatcacc tgagcctggt ccagattgcg caacgaaatg
     4261 ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
     4321 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
     4381 tgttgtagcg gggcaaccag cgcctgaaca atatcctggc aaaagccttc actttgcaag
     4441 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacacctgtc
     4501 tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
     4561 aacggcagcg taatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
     4621 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
     4681 tccattttgt tcttcccgtg atgcagacat aattctatga aagcataaat taaaaacgca
     4741 cagaagcgta ggacgtaatg tc
//
LOCUS       DAZKGS010000247         4700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKGS010000247 DAZKGS010000000
VERSION     DAZKGS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..458
                     /locus_tag="ACHOQY_004648"
     CDS             <1..458
                     /locus_tag="ACHOQY_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429641.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFP9680591.1"
                     /translation="DKSEIKMVDYTQQQKIGAIWKEHLDGWSLAPEHLKTITEKTNIG
                     SLSDTEKAEILFCLGAVFCKYSSSDIFGTEHESPEILRRYANGLLEEAYKLASEIFNK
                     REFYDDIRDRLQGRNNAFSCTAVLADILTSHAKGNFTDIFEQYYPLAWR"
     gene            685..1698
                     /gene="nleC"
                     /locus_tag="ACHOQY_004649"
     CDS             685..1698
                     /gene="nleC"
                     /locus_tag="ACHOQY_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP9680592.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFSPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNTLRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(1819..1902)
                     /locus_tag="ACHOQY_004650"
                     /pseudo
     CDS             complement(1819..1902)
                     /locus_tag="ACHOQY_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            1987..2061
                     /locus_tag="ACHOQY_004651"
                     /pseudo
     CDS             1987..2061
                     /locus_tag="ACHOQY_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(2848..3282)
                     /gene="uspF"
                     /locus_tag="ACHOQY_004652"
     CDS             complement(2848..3282)
                     /gene="uspF"
                     /locus_tag="ACHOQY_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9680593.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(3423..4561)
                     /gene="ompN"
                     /locus_tag="ACHOQY_004653"
                     /pseudo
     CDS             complement(3423..4561)
                     /gene="ompN"
                     /locus_tag="ACHOQY_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
BASE COUNT         1349 a          945 c         1078 g         1328 t
ORIGIN      
        1 ctgataaatc agaaataaaa atggttgatt atactcagca acaaaaaatt ggggctatat
       61 ggaaagagca tcttgatggt tggagtttag ctccagaaca tttaaaaaca ataacagaaa
      121 aaactaatat agggagtctt tcagatacgg aaaaggctga gatattattt tgtttaggtg
      181 ctgttttttg taagtactcg tcatctgata tatttggaac ggagcatgag tctcctgaga
      241 tcctgcgtcg ttatgcaaat ggtcttttgg aagaggcata taaactggca tctgagatat
      301 ttaacaaaag agagttttat gatgatatca gggacaggtt gcaaggacgg aataatgcat
      361 tttcatgcac tgcggtttta gctgatatat taacaagcca tgcaaaggga aattttactg
      421 atatttttga gcagtattat ccattagcat ggcgttgatg tctgttgcta cgtgatgcca
      481 atatatattg caaatcacag ggcagacagc tcattaaact tggtaagctt tgctacgata
      541 aggaaggttt accattgtgg tgtcattagc tcacatgtgt atgggagctt taaacgttcc
      601 tgttactcgt tggaacacct gccttgcagg gataaaagct atgctgtccg ttatcgtctg
      661 ggctagtgaa ttggtggcac tgaaatatat aaaaccatat taagtatcaa tatgaaaatt
      721 cccgttctcc aacctggctt caactttttt tcccctgctg gatactctgc tgccgttgct
      781 cctaatcgtg ctgaaaatgc ctatgcggat tacgttttgg atataggcaa gcgaataccg
      841 ctttccgcag cagatttaag caacgtatac gaaagtgtaa ttcgcgccgt ccatgacagc
      901 cgtagcaggc tcatcgatca gcatacggtc gatatgattg gcaacactgt acttgatgcg
      961 ttgagccgat cacaaacatt tcgtgatgcc gtaagctatg gcattcataa tgaggaggta
     1021 cacattggtt gcattaaata cagaaacgaa tacgagctta acggagaatc tgctatcaaa
     1081 attgatgata ttcaatcact aacctgtaac gaattatatg gatacgatgt cgggcaagaa
     1141 ccaattctcc ccatttgcga ggcaggagaa aacgagaacg aagagcctta tgtcagtttt
     1201 agtgttgcgc cagatactga ttcttatgag atgccatcgt ggcaggaagg actgattcac
     1261 gagattattc atcatgttac tggggccagc gatccatctg gagatagtaa tatagagcta
     1321 ggacccaccg agattctcgc acgtcgtgtc gctcaagagc tgggatggag tgttcccgac
     1381 ttcaaaggat atgcagagcc agaacgagaa gctcatctta gactacgtaa cctgaatacc
     1441 cttcgacagg ctgccatgag gcatgaaaag aatgagaggg ctttcttcga aagactgggt
     1501 acgatcagtg accgatatga ggcgagtcct gatttcacag agtattccgc tgtgtctaac
     1561 ataggatacg gatttatcca gcaacatgat tttccagggg tggctatcga cgagaattta
     1621 caggatgtaa atcagatcca actgtatcat ggtgctcctt atatctttac atttggggat
     1681 gtggacagac acaattagcg ctgattcgtc tttacagtta catagggtta ctactcatag
     1741 atattaacgg agttgatgat gggcaatcgt gcaacattgt attgtatgta aaggcatagc
     1801 cccgaatttt actgttgtaa gtgggcctag cgggaatggt atcgccagca tcattggctt
     1861 ggggccagta aaaagagacg cagaaagatg cacaataggc actacgcgtc atgcatggat
     1921 taggtcgctc atcatttaca cagtactgct aaaaaattat taaggaaagc gcggcacttc
     1981 gtatgtaaga acgtgtcgcg gttggctggt gaactttcga tagtgcgagt attgaatgat
     2041 ttccagccgt taccgatttt atataactgc agtggaatta ttcaacacac aggaaaaacg
     2101 gaataagctg agaaaaaaag cgtgcttacc caatctgggg taaatcacgt ctgaatgaca
     2161 cctgaaaaca ggtggtggcc tcaagaaggc ttggaatatt cttctttaat gttatgtaat
     2221 ttattgattt ttcgtgtacg attttaagac atttattcca agaaaaattt ttaactcctt
     2281 gaattttcga ctctgtatca tcggtctcga aacccgtagc cacgtcgtat gcaagaacgt
     2341 gccgcggttg gctggtaagc ttttcgatag tgcgagtatc taatgatttc cagccggaac
     2401 tgattctatt ggaaagaaat catgggcgca atttacagat cacagattga ttagttatag
     2461 atttttactg tggcaaaaat gggacaaaag gccgcaaaag gggcaaaaat ggggcaaaaa
     2521 aagtgtagat tattgtgctt tattgtagtt gttaatcctg cctaacatat tgaaaaacat
     2581 aaaagttatt gttcgtcaga tagttgcggt ttttactctg acttgttcag gttgtattgt
     2641 tctttcttac taatttcttg attttgcgac atttaaaagc gactcaattc gttatatggc
     2701 atcagaagag tatgcgtcat gccggaacgc ccagcataag aaatctgata taaaaactgt
     2761 ggcgtgtatg gtacggatta gaggggaaaa tgtcagcaca tttgcgaaat gaatcaaaaa
     2821 gcccgcagca atgtgcgggc gttagtgtca gcgcacaacc agcacggagc actctgcgtg
     2881 acgcactaca gctgcggcgt tagaaccgag cagataagtg gtgatatccg gtcgatggga
     2941 agcaatgatg atcatatgag cggggatctt cttcgccaat tccagaatgc ggtcttttgg
     3001 cgagccttcc tcaacatgga catgcactct gtcggttggc agtttaaatt ttttaatgat
     3061 ctcttccagt tgcgatttgg cttccgcttt caggtcatcc attgccggta attctgcgga
     3121 atacgctaaa cccagagagg catagtaggg cagtgaaggt attaccgtca ggaaatgaac
     3181 ctctgcatca tcaatctttg cctctgcctc aacgtggcta atcacgcgtt gagttaattc
     3241 tgaatcggaa atatcgatag ggacaagaat cgttctgttc ataaaacctc ctgttttagt
     3301 atccgcataa agtgtaacgc cagatgacac tttttgtgta atgacggagt tcacattttt
     3361 aatttagatc aaaggaggaa gaataagcag aaaaagcccg ccataacagc gggcaggagg
     3421 atttagaact gataaaccag acctaaagcg acaatatcat cggtagagat gccgttggca
     3481 gcgtagaaac tgtcatcttc atccaacagg ttgattttat agtcaacata agtggacatg
     3541 tttttattga aatagtaagt cgcgccaaca tcggcgtatt taaccagatc tttatcatca
     3601 acacctgccg ggttgtctgc accaccggca gcgtgcaggt cacggccttt agacatcagg
     3661 aaagagactg ccggacgcag accaaaatca aactggtact gtgcagtgac ttcaaagttc
     3721 tgggttttgt ttgcaacagc ataatcgctg tcgccaaacg gggtcatatt acgcgtttct
     3781 gaatacatgg ttgccaggta aatattgtta gcatcgtatt ttagcccagc agtccacgcg
     3841 tctgctttat caccacccgc cgcagtatgg ttaacctggt cattggtgcg gtcagaagag
     3901 gtgtatgccg caccagcgct aaagcccatg cctaaatcat atgttgtgga aagaccccac
     3961 cccagccgtc accgttttca tggcgaacat cacgtccgtt gttggtgcct tcctgaccat
     4021 tactggcacc ttcgttgtta ccttgatact gcaccgcgaa gttcagacca tttaccagac
     4081 cgaagaaatc agtattacga taagtcgcga cgccattggc tcgaccagtc ataaagttgt
     4141 ctgcattggt ataagagtca ccgccaaatt caggcagcat atcggtccag ccttcgatgt
     4201 cgtacattac gccataatta cgtccgtaat cgaaagaacc gtaatctgca aatttcagtc
     4261 cggcaaatgc cagacgggtc catgactggt tttttgaaga ttcagtgttg tttgcctgaa
     4321 tattgtattc ccattgaccg tagccagtaa gttgatcgtt aatttgggtt tcgcctttaa
     4381 aacccagacg cgcatagctc tggtcgccat ctttcgctga attatcagaa aaataatgca
     4441 ggccatcaac tttgccatac agatctaatt tgttgccgtc tttattataa acttcggctg
     4501 catgtgcagc acctgcggcg agcagggcag gaattaaaag tgccagtact ttgcttttca
     4561 ttgaataaat cctttagtta ttttatttgc cttttatccc cattttgggg agtgaaaaca
     4621 tcctaaagga gaagttcagt tagccgataa atatcatttg ttacgcctta agtaaaacct
     4681 taatcaaata attctcctga
//
LOCUS       DAZKGS010000248         4692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKGS010000248 DAZKGS010000000
VERSION     DAZKGS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            29..787
                     /gene="paa"
                     /locus_tag="ACHOQY_004654"
     CDS             29..787
                     /gene="paa"
                     /locus_tag="ACHOQY_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP9680594.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1123..1278
                     /gene="hokD"
                     /locus_tag="ACHOQY_004655"
     CDS             1123..1278
                     /gene="hokD"
                     /locus_tag="ACHOQY_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9680595.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1499..1756
                     /locus_tag="ACHOQY_004656"
     CDS             1499..1756
                     /locus_tag="ACHOQY_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680596.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1826..2104
                     /locus_tag="ACHOQY_004657"
     CDS             1826..2104
                     /locus_tag="ACHOQY_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680597.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2106..3161
                     /locus_tag="ACHOQY_004658"
     CDS             2106..3161
                     /locus_tag="ACHOQY_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9680598.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3162..3527
                     /locus_tag="ACHOQY_004659"
     CDS             3162..3527
                     /locus_tag="ACHOQY_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9680599.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3524..4213
                     /locus_tag="ACHOQY_004660"
     CDS             3524..4213
                     /locus_tag="ACHOQY_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9680600.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4409..4484
                     /locus_tag="ACHOQY_004661"
     tRNA            4409..4484
                     /locus_tag="ACHOQY_004661"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4442..4444,aa:Ile,seq:cat)
     gene            4575..4648
                     /locus_tag="ACHOQY_004662"
     tRNA            4575..4648
                     /locus_tag="ACHOQY_004662"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4609..4611,aa:Arg,seq:tct)
BASE COUNT         1249 a         1061 c         1249 g         1133 t
ORIGIN      
        1 tcatgataac caataaaagg agttttttat gaggaacata atggcaggtt ttttaatatt
       61 cctgtcttct gctgcttatg ctgatatcaa tctgtatggt cctggtggcc cgcatacagc
      121 cttgcttgat gcagccaaac tttatgccga aaaaacaggt attatagtga acgttcatta
      181 cggcccacag aacaaatgga atgaagatgc caaaaaaaat gcagatatct tgtttggcgc
      241 atcagaacaa tctgctctgg ctatcattcg ggaccataaa gacagcttca gtgaaaaaga
      301 tattcagcct ctttatctgc gaaaaagtat tttactggta aagaaaggta atcctaaaaa
      361 tatccggagt attgacgacc tgaccagacc tgggattggc gtaattgtta atgatggtgg
      421 tggtaccagt aatacatcag gcactggcgt ctgggaagat attgccggac gtaaagggaa
      481 tatagaaact gtcgccgcaa tccgaaaaaa tattatttta tatgcgccca atagcggaac
      541 tgcacgtaag gctcttgaga atcagcctgg agcagatgtc tggataacct gggctgactg
      601 ggcagccagt aatccagaaa ttggtgatgt cgtggaaata gcgccagact acgtgatatg
      661 gcgtgatatg aacattacag tacgtcagga tgcaaatgat gaaacccgtc gatttgcaga
      721 atggctacaa accgatgaag cggcgcctgc attcaaaaaa tatggctgga ccaggaaagg
      781 cacttgacat cctcgtcctt caggacgtgg attctttttc cggatgccgc gccagcggca
      841 tgtaggggca gctcacaaaa cggaaaaaat tgtacgctaa gcctcgccag gtgaactgaa
      901 ttcattccga tatgggaatt cccatatcgg gcgaaaacgg tttgctgtaa cggcagagtt
      961 aagtaggatt gctgcgggtg cttgaggcta tctgcctcgg gcatgaacac caacggcaga
     1021 tagataaaag ccccacccga ctataaatcg aagtgaggcc cctatatgct cgtcacatat
     1081 agattgcctc ttacggaccg aaaggtcaag gagaagcagg ctatgaagca gcaaaaggcg
     1141 atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg
     1201 aaagacctct gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct
     1261 tacgaacctg aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctgatgtgt
     1321 caggcatcct caacgcaccc gcacttaacc cgcttcggcg ggtttttgtt tttattttca
     1381 acgcgtttga agttccggac ggcgccggaa tagaatcaaa aatacttaag tagcgcgcag
     1441 ggagaagagg gatggacccc gaacagggga gtgctattta tctggaagga ttctgttgat
     1501 gagaatcgaa gaattacgtg aaatttttag tgaagatggc ctctatactg tgcgcgttga
     1561 gaagggcgct attgtcagcc actgccgtat taaatgttta cagtctcaac aaaggaagag
     1621 tggagctgcg ttaattcatt ttgtggatgg gcttgtgacg gatggtttta ttttgcgtgc
     1681 aaatgaattt gtcacatcgt tgccgtctct gaaagaagct gggattaagg ctggtttttc
     1741 tgcttttgaa gattagtgaa ttcatctaca attcagcgca gggctgaacc cctgttgagt
     1801 aacactgtgc caccggagaa agccgatggc gcaaaattcc agactacaca attctgataa
     1861 ttcagccgtc tttgccagca ggcacgggcg gcgttctcat gcattcaaat ctgactggtt
     1921 ccggcacgcc ccatgcactg aagaacaggc cgaatggctg attcagaact accgcagacg
     1981 tgggtatgag tttaggaaag ccctcagcct cgattatcgt cactggataa tctacgtcag
     2041 gctgccgtac tccgagcgcc caccgcgtcc gtcccgcaca ttccagcaac gcatctggag
     2101 gtaacgtgcg ggtattactt cgacctgttc cggtaccgga acttgggttg gtggtcctta
     2161 aaccaggccg tgaatccatg caggtattcc ataaccctcg agtgcttgtg gagccggaac
     2221 cgaaaagcat gcgcggtctg ccgtccggca tcgtccctgc cgttcgccag ccgctggcgg
     2281 aggataaatc attactgccg tttttcagca atgagcgtgt gattcgtgct gttggtggcg
     2341 ctggtgcact gtctgactgg ctgttgcgtc atgtcaaatc ctgccagtgg cctcatggtg
     2401 actatcatca cagtgaaacc gttattcacc gttatggtac cggcgcaatg gtgttgtgct
     2461 ggcactgcga caaccagctg cgtgaccaga catccgaatc actcgagcaa cttgctcatc
     2521 aaaacctgtc agcatggatg attgacgtca tcggtcacgc aataagcggt acgcaggagc
     2581 gtgaattatc tctggctgaa ttatcctggt gggcggtccg caatcaggtg gcggacgcgc
     2641 taccggaagc ggtattacgt cgttcgctgg ggttgcgtgc ggaaaaaatc cgctcaatgt
     2701 accgtgaaag cgacatcgta ccgggagagc agaccgccac catcatactg aagcagcgca
     2761 caaaaaatct tgcgccgctg cctcacgccc accagcaaaa cccgccacag gaaaagacgg
     2821 tggtcagcat tgccgttgat ccggagtcac cggctcagta tctccagcgc cagaaaccac
     2881 aacgggaaga gatgcctgta tacacgcgct gggtaaaaac gcagaaatgc atgacgtgcg
     2941 gtaatcaggc agatgatccg catcacatca ttggtcatgg actgggaggg atgggaacaa
     3001 aggctgatga tttgtttgtt attccgctgt gccgtaaatg ccatagcgaa ctacacgccg
     3061 gggtaaaaga ttttgaagaa aaacacggca gccagctgtt gttgctgatt cgttttttaa
     3121 tgcacgcgag aaattcgggt gtcctgaagt ggaaagcatg aatgactgaa cggatagaat
     3181 ttgttttgcc ttacccgccg acggtgaata cctactggcg acgtcatggc aatacgtatt
     3241 tcatctcgga agccggaaag cgttatcgcc gtgatgtggc gctaattgtt cgccagcagc
     3301 ggctgaaatt aaacctgtcc ggaaggctgg tgataaagat tattgcagag ccaccggata
     3361 agcgccgtcg tgacctggac aatatcctga aagcaccact ggatgtgctg acgcatgccg
     3421 gacttctcat agacgacgag cagtttgatg aaatcaatat tgtgcgcggt cagctcgttc
     3481 ctggtgggcg gctggggata aaaatcacag aactggagtg cgcatgaata accactattt
     3541 acagtttgtg cgtgagcagc tcattatcgc caccgctgat ttgagtgggg caacaaaagg
     3601 tcagcttgaa gcctggcagg agaatgccat gttcgataca gggcgttaca ggcgaaaaaa
     3661 aatccggtac cgcgatgaag tgactggaaa aatgataacg cgggataatc caccaatccc
     3721 gggaaagcaa tcgctggcga aggtgacgtc aattcctctg gtcagtccgg ttgagttttc
     3781 gacatcatcg tggcggcggg ctgttctgtc tcttgaagaa catcataaag cctggttgtt
     3841 gtggtgttac agcgggagta tttgttggga atatcagatc gcgataacac agtgggcgtg
     3901 gaatgaattt aatactcaat ccggtaccag aaaaattgct gggaaaacgc aggaacgcct
     3961 gaaaaaatta atctggctgg cggcgcaggc agtaaaagca gaactttttg gtggggaagg
     4021 ttatgaatat caggagctgg cattactggc gggagtgaca actaaaaact ggtccaaaac
     4081 atttactcgt cactgggttg caatgaaaca catttttcac cgactggata gtgaggcttt
     4141 attatttgta atgagaacac gttcaaaaca aaaggcggca ttttcaaagc aaagtgttgc
     4201 aaaagtagat tgaaaggcat atatttcatg caaatctgat attttgccga ttttgtacgt
     4261 gatggcaaaa gcaaacaaaa cccgcccaca agcgggtttt gttgtgccac ttatctcgga
     4321 tagacatggt gaatgcgctg gtggaggaga taagggtgat ttttgtatgc ttgcaacatt
     4381 gatttcgtaa cgttattatc ctgcgcccgg ccctttagct cagtggtgag agcgagcgac
     4441 tcataatcgc caggtcgctg gttcaaatcc agcaagggcc accaaccgcc actagccatc
     4501 aggaaagagc gtcaaccctt taagttgagt gtgcgaggtt cgagtccccg gtggtggtcc
     4561 attatcggta ttctgcgttg ttagctcagc aggacagagc aattgccttc taagcaatcg
     4621 gtcactggtt cgactccagt acaacgcgct acacttattt tcccggctcg cttttgcggg
     4681 ccttttttgt at
//
LOCUS       DAZKGS010000249         4653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKGS010000249 DAZKGS010000000
VERSION     DAZKGS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            419..1435
                     /locus_tag="ACHOQY_004663"
     CDS             419..1435
                     /locus_tag="ACHOQY_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680601.1"
                     /translation="MSSVRTDKFIRYGFAVVAIVAILITTITTPNPTPYQYIVYRIVL
                     VLAAGCIGAVLPGFLEVKFNGWLRASGAVAIFAIVFFISPDLLQQNPESASKPVPPTV
                     IDAYAVNSSNRDRYLELFRTGQFGQRDKLRIGCVEWSESSCVAAGNFLRLLSEAGWEI
                     DSDAVFRMQPSVPVEGVSIVSRGDDLVGLPKVPPHMGRWSRSDISYEILTAAFKLMGV
                     PVYFSSDPTLQPGTTGVYFGPKPKLDAPVMQKDENVRRELLRYVGLGMTIQRACSQQP
                     VELCGGEIFSWETSVSSFLRQQRFNSDAVNRWLALPQVNAASATADMKIKLDLLMSFV
                     LGIE"
     gene            2079..4439
                     /locus_tag="ACHOQY_004664"
     CDS             2079..4439
                     /locus_tag="ACHOQY_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFP9680602.1"
                     /translation="MKSMQSKTGSPLVRTEAELESRLTSALNIAFPNIPREDLIEQRH
                     FTVRLGHGTYKIDSAAHWKKYGRADVLIFHRERPLAVIELKREDLTLTHDDYEQAQSY
                     ANQLTPRPPLVVVTNGKDTRVYDSSNGQQWSGGQDASAAVNKMLANSAKLAAADMRWA
                     IEALMGRETNAWVPAVREETARLLIDITDQPGHSEHPFANNLLFPRKITSLVIESAVM
                     GTAFTIIEGDAQSGKSSCLREISLKTESSDLLAVLMLRGSGPGLFQALANLFAAEFEW
                     NLTSNDARNWLRRMSNCTEGPSLMLAIDDVEPGSQMATDLEELAGIRFGNRLVVVLTT
                     YHANALLKNPNGRTPSAIGSRSKVFKTSPMSLDEFKLAQQILSDQRIVFQQGAEYADD
                     YRSPWVLRTIYDDIVRNHQYQKTDLIAYLPPSPGMELIDAAQKSYESQYDLLRYYRVL
                     ARCALADTNSHSVELMFAKANGFVVRYDALSDEARGVVNELKHMGAVRIFRLSGWEDV
                     VVPTVPAAYLLELSDAVCDELVLRAEQDPQDAGAWLGERLDATYLGDMIGAQAIRRMA
                     AKERYFSFGIIQGLLSIEPYKEPIKNGLFTLAMPDNQQVNLKIEDGLAWISKHGDDAK
                     SVLVNLEDQIPKVISKSTSWMILGQLAKLPSAEVGDDDQRIDAYILLSIGRCPFPLIR
                     TNIEGLPYFEHNFGDQGDVLCLEKASIEVATQAMADLFSAPWLYADQWVDTAIATGSI
                     HLLHRLFAALNTVILRRIPVQSDWANEALNQRVSPALKEAIRSLSS"
BASE COUNT         1224 a          998 c         1128 g         1303 t
ORIGIN      
        1 acttacttgg ctttagtccc ggagtgttgc caggagtatc tttattatct cgtcggcgtt
       61 gatctggttt tcctgtcgat ttagcaattg gttttggacc aggtttaaga cccatgatag
      121 ttatcctttt gagatgtaag agggaagccc tcaacattgg tatattggat actgagaaat
      181 ttttcaatgg gagtgataaa cggccgaatg attaactcga tattgtgatc aacgtctgtt
      241 ttttgcacac tgtagactga ctcttggagc agaaggttgc tctgagcgag gaatggacag
      301 ttacactcat caccaacgcc aatagcctca ttacgcgtat aaagctgtat taatcattgg
      361 ggagcagttt aatgcgggat tttcgactta tggacttgat tcaggtgtca aaattaaaat
      421 gagcagtgta aggacagaca agttcattag gtatggtttt gcggttgtgg cgatagttgc
      481 catactcatt actactatca caacccccaa tcccacacct tatcaatata tagtttaccg
      541 aatagttctg gttctggctg ccggatgtat tggtgcagtt ctgcctggtt tccttgaagt
      601 aaaatttaat gggtggttaa gagcatctgg cgcagtggcg atcttcgcta tagtgttttt
      661 cataagccca gatttgcttc aacaaaatcc tgaatctgca agtaaacctg taccgcctac
      721 tgtgattgat gcatatgccg ttaatagttc caatcgcgac agatacctcg aattatttcg
      781 aacaggacag ttcggtcagc gggacaagct gcgcatcggt tgtgttgagt ggagtgaatc
      841 atcgtgcgtg gctgctggta attttttgcg attgttaagc gaagctggct gggaaatcga
      901 ttctgacgct gtattcagaa tgcaaccatc cgtgccagtt gagggggtat cgattgtaag
      961 ccgtggcgat gatctggtcg gtttgccaaa agtaccaccc cacatgggcc gttggagtcg
     1021 atcagacatt agttatgaaa ttttgacagc ggcatttaaa ttgatgggag ttccggttta
     1081 tttttccagt gacccgacat tacaaccagg tactaccgga gtgtactttg gaccgaaacc
     1141 gaaactggat gcaccagtca tgcaaaaaga tgagaatgtg cgacgtgagc ttctccgcta
     1201 cgtcggtttg ggaatgacta ttcagcgagc ctgctctcag cagcccgtcg aactttgtgg
     1261 gggtgaaatc ttttcctggg aaacgtcggt atcttcgttc ctaaggcagc agagattcaa
     1321 ctccgatgcc gtaaacagat ggcttgcctt gccgcaggtt aatgcggctt cggcaactgc
     1381 agacatgaaa ataaagctag atttgttaat gtcatttgtt ctgggcatcg agtaactata
     1441 cctcttgcga ggatgattga gcgtctaatg gaccagctta gccgttagcg ctatttgttt
     1501 acgtaagcct gtggaatcca actagtgctt ctacgatgcg ttgtttggtg aaaaaaaagt
     1561 gttgaagcac tggttttaga caactaaagc cacgatttgc tcatgcgtcg aaaatgtagt
     1621 cgtctactgt gtagcacgga ggcgatattc gtacgcttac acttgccgat aggccactgg
     1681 ggagtgttct tggcactaag ctgccaggcc cgaaggtctg ctatgagcga ggggctgaca
     1741 ttcctattgg cattcggtgt gagttatcgg gggcggtcat gcggcatact tccgatacag
     1801 gcgtttccag ctctgcctgt ttcagggtta tattaatctg ctttagaatt aatcgtggct
     1861 ttagcatctg cacatcgtgc tggagaaacc tgagtgaact aaatcatatt ccctttcttc
     1921 agctaatcaa ttgatagtta tgcggaaaaa gttactttgc ttgtcaataa tccatttcac
     1981 atccgttaat ccaatgctat gcgtcttcgc attaatctgg aaaggtagta agtgaaataa
     2041 aaacttagcc aatgactaat atctgaggat actttgtcat gaaaagtatg caatcaaaaa
     2101 cgggctctcc actcgttcga actgaagccg agctagaaag ccggcttact tcagcactaa
     2161 acatagcatt tcctaacatt cctcgcgaag atcttatcga acagcgccat tttaccgttc
     2221 ggctaggcca tggaacttac aaaatagata gcgccgcaca ctggaaaaaa tacgggcgag
     2281 cggatgtttt gatctttcat cgggagagac cacttgcggt tattgaactg aaacgtgaag
     2341 acctgacact gacccatgat gattatgaac aggctcaaag ctacgctaac cagttaacac
     2401 cacgtccgcc tctcgttgta gttacgaacg gtaaagatac gcgcgtttat gactcaagta
     2461 atggacaaca gtggtcgggt ggacaggatg cctcggcagc ggtaaacaag atgctcgcca
     2521 attctgcaaa gttggcggcg gctgacatgc gctgggctat tgaagccttg atggggcgag
     2581 aaactaatgc ttgggtgcca gctgtgcgag aagaaacagc ccggctgctg attgatataa
     2641 ctgatcagcc tgggcattca gaacatccat ttgccaacaa cctacttttt cctcgcaaaa
     2701 tcacgtctct tgtaattgaa tctgctgtga tgggtacagc tttcactatc attgagggtg
     2761 atgcacaaag cggaaaaagc agttgcctcc gtgaaatttc gctaaaaacg gaaagtagcg
     2821 atcttttggc tgtcctgatg ttacgaggtt ccggaccagg actatttcag gctctggcca
     2881 atctttttgc agctgaattt gaatggaatt taacctcaaa tgatgcacgt aactggttaa
     2941 gacgaatgtc taactgcact gaaggtccat cgcttatgct tgctatcgac gacgttgaac
     3001 caggctccca aatggctact gatcttgagg aacttgcggg tatacgtttt ggcaacaggc
     3061 tggtggttgt tctgacgacc tatcatgcca acgcgttact gaaaaatcca aacggacgaa
     3121 ctccgtctgc aattggtagc cggtcgaagg tatttaaaac atcgcctatg tccctagatg
     3181 aatttaagct ggcgcaacag attctctcgg accagcggat agtttttcaa cagggagcag
     3241 aatacgccga tgactaccgc tcgccttggg ttcttcgcac tatttatgat gacattgtgc
     3301 gcaatcatca ataccagaaa acggatctta tcgcctatct tcctccatca cccggtatgg
     3361 aacttataga tgctgcgcaa aagagctatg agagtcagta tgatcttctt cgttattatc
     3421 gggttcttgc acgctgcgca ctagctgaca caaactccca ctcagtagaa ttaatgtttg
     3481 ctaaagcgaa tggatttgtc gtgcggtatg acgcactttc tgacgaagct cgtggtgtag
     3541 tgaatgagtt aaaacatatg ggggcagttc gtatttttcg cctttcagga tgggaagatg
     3601 ttgtggtgcc gacagttccc gcagcttacc ttctggaact gtcggatgct gtttgtgatg
     3661 aactggtttt acgggcagaa caagaccctc aagacgccgg ggcttggctt ggcgaacggc
     3721 tagacgccac ttatcttgga gatatgatcg gtgctcaagc catacgccgt atggcagcta
     3781 aagagagata tttcagcttt ggaattattc agggccttct cagcattgag ccatataaag
     3841 aacctataaa aaacggattg tttactttag ccatgccaga caaccaacaa gtcaatctaa
     3901 agattgagga tggattagcg tggatttcta aacacgggga tgatgcaaag agtgttctgg
     3961 tgaatttaga ggatcaaatc ccaaaggtca tttctaaatc aaccagttgg atgatactcg
     4021 ggcaactagc gaaactgccg tcagcagagg tgggtgacga tgatcagcgt atagacgcct
     4081 atattttact gagcattggg cgctgcccat ttcctctaat tcgcacgaat atagaaggat
     4141 tgccttactt tgagcataat tttggcgatc agggtgatgt tctttgccta gaaaaagcgt
     4201 ctattgaagt tgctactcag gcgatggctg atttattttc agccccatgg ctctatgcgg
     4261 accagtgggt ggatacagct atcgcgaccg gttctataca tttactgcat cgtttgtttg
     4321 ctgctttaaa cacagtgata ttgcgtagaa tacccgtgca atccgactgg gctaatgaag
     4381 cacttaatca acgtgtctca ccagccctta aggaagccat acgatcatta tcttcgtgac
     4441 taagcaatgg gattttaata cataacgagc atctgtccac gcaagttaac aattgccttg
     4501 gctcgatatt ttggtttcta gtgcatatat gctgatcttc tcttcgttga tctgatctta
     4561 cccaccaata gtggacacag gactaagtga gtaaactctc aaccagaggt gactcatgac
     4621 aaaaccagta tcaatcagca agaagccccg tga
//
LOCUS       DAZKGS010000250         4539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKGS010000250 DAZKGS010000000
VERSION     DAZKGS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            283..4536
                     /gene="fdeC"
                     /locus_tag="ACHOQY_004665"
     CDS             283..4536
                     /gene="fdeC"
                     /locus_tag="ACHOQY_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9680603.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         1193 a         1068 c         1270 g         1008 t
ORIGIN      
        1 attcactgtc cgcagagtaa gaaatataac ttaggtatct atttaatgac ttgcacaaaa
       61 agctaaattt tcccccataa ataaaaatat aatcccgcgc ccaaccacct gatgagtggc
      121 tacaggcact ggatatatta ggtggcggtg cactttctta cataaaggta tttccttttc
      181 tgcggaaaag gaaatcggga aatccccggt ttttctgaca agcagacgcc attatttgtg
      241 tctgcctatg ttcgttaatt cgttcatcag gaaattatct caatgtcaca ttataaaaca
      301 ggtcataaac aaccacgatt tcgttattca gttctgaccc gctgcgtggc gtgggcaaat
      361 atctctgttc aggttctttt tccactcgct gtcaccttta ccccagtaat ggcggcacgt
      421 gcgcagcatg cggttcagcc acggttgagc atgggaaata ctacggtaac tgctgataat
      481 aacgtggaga aaaatgtcgc gtcgtttgcc gcaaatgccg ggacattttt aagcagtcag
      541 ccagatagcg atgcgacacg taactttatt accggaatgg ccacagctaa agctaaccag
      601 gaaatacagg agtggctcgg gaaatatggt actgcgcgcg tcaaactgaa tgtcgataaa
      661 gatttctcgc tgaaggattc ttcgctggaa atgctttatc cgatttatga tacgccaaca
      721 aatatgttgt tcactcaggg agcaatacat cgtaccgacg atcgtactca gtcaaatatt
      781 ggttttggct ggcgtcattt ttcaggaaat gactggatgg cgggggtgaa cacctttatc
      841 gaccatgatt tatcccgtag tcatacccgc attggtgttg gtgcggaata ctggcgcgat
      901 tatctgaaac tgagcgccaa tggttatatc cgggcttctg gctggaaaaa atcgccggat
      961 gttgaggatt atcaggaacg cccggcgaat ggctgggata ttcgtgctga gggctattta
     1021 cccgcctggc cgcagcttgg cgcaagcctg atgtatgaac agtattatgg cgatgaagtc
     1081 gggctgtttg gtaaagataa gcgccagaaa gacccgcatg ctatttctgc cgaggtgacc
     1141 tatacgccag tgcctcttct gacactgagc gccgggcata agcagggcaa gagtggtgag
     1201 aatgacactc gctttggcct ggaagttaat tatcggattg gcgaacctct ggcgaaacaa
     1261 ctcgatacag acagcattcg cgagcgtcgg gtactggcag gcagccgcta tgacctggtt
     1321 gagcgtaata acaacatcgt tcttgagtac cgcaaatctg aagtgatccg tattgctctg
     1381 cctgaacgta ttgaaggtaa gggtggtcag acactttccc tggggcttgt ggtcagcaaa
     1441 gcaactcacg gactgaaaaa tgtgcagtgg gaagcgccgt cattactggc tgagggtggc
     1501 aaaattaccg gtcagggtag tcagtggcaa gtaacgctcc cggcttatcg tccaggcaaa
     1561 gacaattatt atgcgatttc tgcggttgcc tacgataaca aaggcaatgc ctcaaaacgc
     1621 gtgcagacag aggtggtcat taccggagca ggtatgagcg ccgatcgcac ggcgttaacg
     1681 cttgacggtc agagccgtat tcaaatgctt gctaacggta atgagcaaag accgctggtg
     1741 ctgtctctgc gcgacgccga ggggcagcca gtcacgggca tgaaagatca gatcaagact
     1801 gaactagcct tcaaaccggc tggaaatatt gtgactcgtt ccctaaaggc cactaaatca
     1861 caggcaaagc caacactggg tgagttcacc gaaactgaag caggggtgta tcagtctgtc
     1921 tttactaccg gaacgcagtc aggtgaggca acgattactg ttagcgttga tggcatgagc
     1981 aaaaccgtca ctgcagaact gcgggccacg atgatggatg tggcaaactc caccctgagc
     2041 gctaacgagc cgtcaggtga tgtggttgct gatggtcagc aagcctatac gttgacgttg
     2101 actgcggtgg actccgaggg taatccggtg acgggagaag ccagccgctt gcgatttgtt
     2161 ccgcaagaca ctaatggtgt aaccgttggt gccatttcgg aaataaaacc aggcgtttac
     2221 agcgccacgg tttcttcaac ccgtgccgga aacgttgttg tgcgtgcttt cagcgagcag
     2281 tatcagctgg gcacattaca acaaacgctg aagtttgttg ccgggccgct tgatgcagca
     2341 cattcgtcca tcacactgaa tcctgataaa ccggtggttg gcggtacagt tacggcaatc
     2401 tggacggcaa aagatgctaa tgacaaccct gtaactggtc tcaatccgga tgcaccgtca
     2461 ttatcgggcg cagctgctgt aggttctacg gcatcaggct ggacggataa tggcgacggg
     2521 acctggactg cgcagatttc tctcggcact acggcgggtg aattagaggt tatgccgaag
     2581 ctaaatggac aggatgcggc agcgaatgcg gctaaagtaa ccgtggtggc tgatgcgtta
     2641 tcttcaggcc agtcgacagt gtctgtagcc gcagatcgcg taaaagcagg cgaaagcaca
     2701 accgtgacgc ttattgcaaa agatgcacat ggcaacgcta tcagtggtct ttccctgtcg
     2761 gcaagcctga cgggtgctgc gtctgaaggg gcgactgttt ctggttggac cgaaaaaggt
     2821 gatggttcct atgtcgctac gctgacaaca ggtggaaaga cgggtgagct tctcgtcatg
     2881 ccgctattca acggccagcc agcagccacc gaagccgcgc agttgactgt cattgcgggg
     2941 gagatgtcat cagcgaactc tacgcttgtt gctgacaata aggctccgac cgtcaaaacg
     3001 acgacgaaac tcaccttcac cgtgaaggat gcgtacggga accttgtcac cgggctgaag
     3061 ccagatgcac cgcagtttag tggtgccgcc agcacgggga cagagcgacc ttcaacagga
     3121 gactggacag aaacaagtaa tggggtctac gtggcgacct taacgctggg atctgccgcg
     3181 ggccagttgt ctgtgatgcc gcgagtgaac ggccaaaatg ccgttgctca gccactggtg
     3241 ctgaatgttg ctggtgacgc atctaaggct gagattcgtg atatgacggt gaaggttgat
     3301 aaccagctgg ctaatggaca atcgactaac caggtaaccc tgaccgttgt ggacacctat
     3361 ggtaacccgt tgcagggaca aaatgtgacg ctgactctgc cgaaaggtgt gaccagcaag
     3421 acggggaata cggtaacaac cgatgcggca ggtaaagccg acattgagct gatgtcaacg
     3481 gttgccgggg aacacagcat cacggcctca gtgaataatg ctcagaagac ggtcacggtg
     3541 aaattcaacg cggatgccag caccggtcag gcaaacctgc aggtagacac cgctgctcaa
     3601 aaagtggcaa acggcaaaga tgcctttacg ctgacggcga acgttgagga taaaaatggt
     3661 aaccctgttc cagggagcct ggtgaccttt aatctgcccc ggggtgtcaa gccgcttaca
     3721 ggcgataatg tctgggtgaa agccaacgat gaggggaaag ccgagttgca ggtggtttca
     3781 gtgactgccg gaacgtatga gatcacggca tcggcaggga atagccagcc ttcgaatacg
     3841 cagactataa cgtttgtagc cgataaggct accgcaaccg tctccggtat tgaggtgatg
     3901 ggcaactatg cgctggcgga cggcaaagcc aaacaaacgt ataaagttac ggtgactgat
     3961 gccaataaca atttggtgaa agatagcgaa gtgacgctga ctgccagccc ggcttcgtta
     4021 aacctggaac cgaatggcac tgcgacaacg aatgagcaag ggcaggctat tttcaccgct
     4081 accactactg ttgcggcgac atacacactc aaggcgcaag tgagtcagac caacggtcag
     4141 gtatcaacga aaactgccga atctaaattc gttgcggatg ataaaaacgc ggtactcacc
     4201 gcatcatctg atatgcaatc tctggtggcg gatgggaaat cgactgcgaa gctggaggtg
     4261 acactgatgt cggcaaacaa ccccgttggc gggaatatgt gggtcgacat tcagacgccg
     4321 gaaggggtga cggaaaagga ttatcagttc ctgccgtcga aaaatgacca tttcgtgagc
     4381 ggaaaaatca cgcgtaaatt tagtaccagc aagcctggtg tctatacgtt cacatttaac
     4441 gccctgacct atggcgggta cgaaatgaag ccagtgacgg tgaccattac cgcggtggat
     4501 gccgatacgg caaaggacga ggaggcgatg aaataattt
//
LOCUS       DAZKGS010000251         4473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKGS010000251 DAZKGS010000000
VERSION     DAZKGS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..97)
                     /locus_tag="ACHOQY_004666"
     CDS             complement(<1..97)
                     /locus_tag="ACHOQY_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9680604.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVR"
     gene            complement(212..523)
                     /locus_tag="ACHOQY_004667"
     CDS             complement(212..523)
                     /locus_tag="ACHOQY_004667"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9680605.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            722..1420
                     /locus_tag="ACHOQY_004668"
     CDS             722..1420
                     /locus_tag="ACHOQY_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9680606.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1465..2364)
                     /gene="eamA"
                     /locus_tag="ACHOQY_004669"
     CDS             complement(1465..2364)
                     /gene="eamA"
                     /locus_tag="ACHOQY_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9680607.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLWWRKAVKVRG"
     gene            2559..3746
                     /gene="ydeE"
                     /locus_tag="ACHOQY_004670"
     CDS             2559..3746
                     /gene="ydeE"
                     /locus_tag="ACHOQY_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9680608.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3873..3968
                     /gene="mgtS"
                     /locus_tag="ACHOQY_004671"
     CDS             3873..3968
                     /gene="mgtS"
                     /locus_tag="ACHOQY_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9680609.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3968..4072
                     /gene="mgtT"
                     /locus_tag="ACHOQY_004672"
     CDS             3968..4072
                     /gene="mgtT"
                     /locus_tag="ACHOQY_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9680610.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4187..>4473)
                     /gene="dgcZ"
                     /locus_tag="ACHOQY_004673"
     CDS             complement(4187..>4473)
                     /gene="dgcZ"
                     /locus_tag="ACHOQY_004673"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9680611.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         1208 a         1059 c          941 g         1265 t
ORIGIN      
        1 ctctcaccgc tgcaacatgt ttttgtgagt ttaacttgat agcaactgga acaagaaaac
       61 gttccattcc acgttcgaat gaggccatta atcccatttt tttatacctg ttgttttgga
      121 tatttataca tcccaataaa gcatgtatgc catatccatt ttatgatgtg tgaaaagtta
      181 gttcaggtaa tagtttaata atattagatt cttactcttg cagtagtttc atcgcaaatt
      241 caagaactgc ctgtccgttc atggtgccgt agtctttcat ctcgataacg gctacaggca
      301 ttttcccctg tgcaacggct tcaatctctg gtttctgaaa gcgaacttgc gggccaagta
      361 aaacaacgtc aacttcgtta tttttaattt tccctttagc ctctgcaatc gccaatgcgg
      421 aaatattaac ttcaagtgaa atagcgttag catgatcaat catacgttta accagcatac
      481 tggttgacat gcccgcagcg caaactaaca atattttttt catattttcc cttactggta
      541 tataacagat tacattgttt aaatatcata tcccgcagta gctatacgtc ctggtacatt
      601 gatcacagta atacagtgat aaagttgttc aacatcaggg tgattattaa acgactcggc
      661 catacgataa accaatcagg actcaactcc ttgttttcaa tggctcttaa aaggtgcaaa
      721 agtgattttt caaaagattg cccgcttact taaatccgaa atcaatggca attcatggca
      781 tgttggtgat ttgttgccgt cagaagcgga actcgctgtt cgttataatg tttcgcgtaa
      841 taccctgcgt aaggcattat ccttgctgga aggcgaagga attattcaca gaaagcatgg
      901 ttcaggaaga tacattcaaa aaaagaattt tgttgcacac attgatcaca tgaacagttt
      961 cagtgaaatt gcgcataaaa gcggcaaaga ggcagggagt cagattatga aatttgaagt
     1021 gcaggatgct tcacccacta ttgcgactga gctgaattta gtgactggtg agcaggttta
     1081 ttacataaag agactgcgat tcattgagga taatgcagca caattagaag aaacgtggat
     1141 gtcagtagca cgttttcctg atttaaccgt gtcacatatg caaaaatcca aattttcata
     1201 tattgagaat gaatgcggga tcaaaatcat tggcaccttt gaaactttct cccctacttt
     1261 tcctacccca gaaatcgcca gtattttacg gatcagccca cgggatccca tacttaaaat
     1321 tcagacccag gctgtggaca gtaactctat tccgctggat tattcgttac tttacagcaa
     1381 tattttcgag ttccaggtaa agtacttttt cccgcgataa aagcagacgc acagagtgct
     1441 gacatcacat tctgtgcgta tggtttagcc tctaaccttt accgctttac gccaccacaa
     1501 gccaaataca ttgatataca gcccggtcat aatgagcacc gcacctaaaa attgcagacc
     1561 cgttaagcgt tcatccaaca atagtgccgc acttgccagt ccgactacgg gcaccagtaa
     1621 cgataacggt gcaacccgcc aggtttcata gcgtcccagt aacgtccccc agatcccata
     1681 accaacaatt gtcgccacaa acgccagata catcagcgac aagatggtgg tcatatcaat
     1741 agtaaccaga ctgtgaatca tggatgcgga accatcgaga atcagcgagg caacaaagaa
     1801 gggaatgatt gggattaaag cgctccagat taccagcgac atcaccgccg gacgcgttga
     1861 gtgcgacatg atctttttat tgaagatgtt gccacacgcc caactaaatg ctgcagccag
     1921 ggtcaacata aagccgagca tcgccacatg ctgaccgttc agactatctt cgattaacac
     1981 cagtacgcca aaaatcgcta aggcgatccc cgccaattgt ttgccatgca gtcgctcccc
     2041 gaaagtaaac gcgccaagca cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc
     2101 cagtccagca ggcataccaa agttaatggc gcagaacaga aaggcaaact gcgcaaaact
     2161 gatggttaat ccatacccca agagcaaatt cagtggtact ttcggtcgtg caacaaaaaa
     2221 gatggccgga aaagcgacca gcataaagcg caaaccggcc agcatcagcg gtggcatgtt
     2281 atgaagcccc actttgatga ccacaaaatt tagcccccat acgaccacta ccagtagcgc
     2341 caacacccca tcttttcgcg acattctacc gcctctgaat ttcatctttt gtaagcaatc
     2401 aacttagctg aatttacttt tctttaacag ttgattcgtt agtcgccggt tacgacggca
     2461 ttaatgcgca aataagtcgc tatacttcgg atttttgcca tgctatttct ttacatctct
     2521 aaaacaaaac ataacgaaac gcactgccgg acagacaaat gaacttatcc ctacgacgct
     2581 ctaccagcgc ccttcttgcc tcgtcgttgt tattaaccat cggacgcggc gctacgctgc
     2641 catttatgac catttacttg agtcgccagt acagcctgag tgtcgatcta atcggttatg
     2701 cgatgacaat tgcgctcact attggcgtcg tttttagcct cggttttggt atcctggcgg
     2761 ataagttcga caagaaacgc tatatgttac tggcaattac cgccttcgcc agcggtttta
     2821 ttgccattcc tttagtgaat aacgtgacgc tggtggtgct cttttttgcc cttattaact
     2881 gcgcctattc cgtttttgct accgtgttga aagcctggtt tgccgacaat ctttcgtcca
     2941 ccagcaaaac gaaaatcttc tcaatcaact acaccatgct aaacattggc tggaccatcg
     3001 gtccgccgct cggcacgctg ttggtgatgc agagcatcaa tctgcccttt tggctggcag
     3061 ctatctgttc cgcgtttccc atgcttttca ttcaaatttg ggtaaagcgc agcgagaaaa
     3121 tcatcgccac ggaaacaggc agtgtctggt cgccgaaagt tttattacaa gataaagcac
     3181 tgttgtggtt tacctgctct ggttttctgg cttcctttgt tagcggcgca tttgcttcat
     3241 gcatttcaca atatgtgatg gtgattgctg atggagattt tgccgaaaag gtggtcgcgg
     3301 ttgttcttcc ggttaatgct gccatggtag ttacgttgca atattccgtg ggccgccgcc
     3361 ttaacccggt taacatccgc gcgctgatga cagcaggcac cctctgtttc gtcatcggtc
     3421 tggttggttt tattttttcc ggcaacagcc tgctattgtg gggtatgtca gctgcggtat
     3481 ttactgtcgg tgaaatcatt tatgcgccgg gcgagtatat gttgattgac catattgcgc
     3541 cgccaggaat gaaagccagc tatttttctg cccagtcttt aggctggctt ggtgctgcaa
     3601 ttaacccatt agtgagtggc gtagtgctaa ccagcctgcc gccttcctcg ctgtttatca
     3661 tcttagcgtt ggtgatcatt gctgcgtggg tgctgatgtt aaaagggatt cgtgcaaaac
     3721 cgtgggggca gcccgcgctt tgttgattta agtcgaacac aataaaaatt taattcagcc
     3781 ttcgtttagg ttacctctgc taatatcttt ctcattgaga tgaaaattaa ggtaagcgag
     3841 gaaacacacc acaccataaa cggaggcaaa taatgctggg taatatgaat gtttttatgg
     3901 ccgtactggg aataatttta ttttctggtt ttctggccgc gtatttcagc cacaaatggg
     3961 atgactaatg aacggagata atccctcacc taaccggccc cttgttacag ttgtgtacaa
     4021 ggggcctgat ttttatgacg gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc
     4081 atggatagat ttgtgttttg cttttacgct aacaggcatt ttcctgcact gataacggat
     4141 cgttgacaca gtagcatcag ttttctcaat gaatgttaaa cggagcttaa actcggttaa
     4201 tcacattttg ttcgtcaata aacatgcagc gatttcttcc ggtttgctta ccctcataca
     4261 ttgcccggtc tgctcttcca atgaccacat ccagaggctc ttcaggaaat gcgcgactca
     4321 cacctgctgt cacggtaatg ttgatatgcc cttcagaatg tgtgatggca tggttatcga
     4381 ctaactggca aattctgaca cctgcacgac atgcttcttc atcattagcc gctttgacaa
     4441 taatgataaa ttcttcgccc ccgtagcgat aaa
//
LOCUS       DAZKGS010000252         4399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKGS010000252 DAZKGS010000000
VERSION     DAZKGS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            511..771
                     /locus_tag="ACHOQY_004674"
     CDS             511..771
                     /locus_tag="ACHOQY_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9680612.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            768..1316
                     /locus_tag="ACHOQY_004675"
     CDS             768..1316
                     /locus_tag="ACHOQY_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9680613.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1316..1540
                     /locus_tag="ACHOQY_004676"
     CDS             1316..1540
                     /locus_tag="ACHOQY_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680614.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1537..1860
                     /locus_tag="ACHOQY_004677"
     CDS             1537..1860
                     /locus_tag="ACHOQY_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFP9680615.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1875..4208
                     /locus_tag="ACHOQY_004678"
     CDS             1875..4208
                     /locus_tag="ACHOQY_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP9680616.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1053 a         1033 c         1134 g         1179 t
ORIGIN      
        1 tgtctgttta ataattaaac attcaacctc cctgcactca gttgcatttc aggaaaatat
       61 tcataaaaat gaatttgtct gatgttcatt aaacacatcc ggatacatgc ggaagtaaga
      121 tggtaattta atttctttaa tcatcggatt tattgataca tatggtgagc tgtttatttt
      181 tggccgtttt ggctctttta ttaatcaggt gttgtcttag tgcttatgat gtaacgccct
      241 gaatgatatg ggctggttca gatttgagct gtgatcctgc ctagtgtaat agactttttt
      301 attattgatg ctccttatta tattccataa aggaattgtt tgtttttgtt cgtttcatac
      361 ggtttttagc tgttttattt tttgagaaat caacatttca cacaaaataa ataaagcatg
      421 cgcgagccgc ttatataaac ataataagga gctacctgaa tccgcatgga attgcccgga
      481 ctgttgtaaa catattagag aggtagccag atgaatagta tttccgtttc caccccggca
      541 ccagctgctc cggtgctccc ggttccggct cagcaggagc gcttcatacg attacctgaa
      601 gtgatgcacc tttgcgggct atcccgttca accatctacg accttatcag ccgcgatgcc
      661 ttcccgcagc agatatccct gggtggaaaa aatgtggcct gggtacagtc ggaggtcagt
      721 gcatggatgg cggcgcgaat tgctgcccgc gagcaaggag gccatgcgtg aaacttgctt
      781 tgcagtctca tcttcttttt tctggcttgc gtcctgatat cttttccagg tatagttttc
      841 ccgctgccgc aaaatcggct gccgggcgta ggaacccgag ttacttattg gcgacaccag
      901 acgcgccgtg cgtctttttt tacgtcgttg ctcaggcaca cccatttttc gggctgcggt
      961 gcttatacct tagccccttt cagataatgg tggtccgggc ggggcagcct tcgggctggc
     1021 cggtttccaa taaggccggt attcctaccc ccgttcgggt caccacccat gagcgtagga
     1081 actccggtgg tggcatttac cgctacttat tggaggttgc ccttatggct acgatcctcg
     1141 ccccgtcaca tcctcaattt atatttgttt ttgccgccgt tcgtcgtgca gaacgtaaac
     1201 cacgtatcag tatgctgcgt atcatcgcca gcaatgagca tcatgcacgt ctttcactgg
     1261 tccatgagta tgtgctttgt cttgctgctc gcctgcctgt ccgggaggtg atctaatgaa
     1321 tcacgccgca atctcttatg acgatattct ccgcctgaag catcttcgta acgtgggaga
     1381 gtttgtcact ggaatggctg ttcttcagga ttgctacgaa aaacctgcca gtgctcaatg
     1441 tgaacaactg gtttccctga tttatctgat gacagagcag cttgatggcg tggtacagcg
     1501 ttgccaggat gacctcatga acatggaggt ggtgcaatga aatcacgcac tctttccctt
     1561 tcacttcggg tagcgttgta tcgccgcgcg gtagcctgcg cctggttgag tgcctgctat
     1621 cagcaaaacc gccatctgca gctcactctg gatgaaattg aaaccgctat tgcccgtgag
     1681 cttgaaggat tttatctgcg acagcacggg caaacaaaag gcatggaaat cgcctgcgcc
     1741 ctgctttcag acctgatgga atacggccct ctaatgtcct gtcccgcact ctcccagctc
     1801 ggaatagccg taatggatga gttatgcgtc cgtcacatca aaaagccggt tttacactga
     1861 gggagaactg cgtcatgtca ggaatgaaag tcagtcaggc cgtgaaggct gcccgcggtc
     1921 actgggctca gattctgcca gcgctgggcg tgaatatatt gaaaaatcgc catcagcctt
     1981 gcccggtctg tggcggtaaa gatcgttttc gcttcgacga tcaggaagga cgcggaacat
     2041 ggttctgcaa tcagtgcgga gccggggatg gtttagcgct tgtcaccagg gcactgaatg
     2101 tggatatcag tgaagcagct gacaggatac atggactgac acatgatctg tctgtagtta
     2161 attccgaggt caggacgtta accgccgata ccgatagcgg gaaagatgca gcggcagcac
     2221 ttgccgcgcg actgctgcaa gcctcgcgtg aatctgccgg aaacacttat cttacacata
     2281 aaggtttccc ggagcatatt tgccatgagc tgacttcagc tcataaggcc ggtggtatga
     2341 tgttccgccc cggtgatttg atcgtcccac tctataacgc tgacggggag ctggtgaata
     2401 ttcagctcat cagtggcaat ggtagcaagt gttttcttaa aggaggtcag gttaaggagg
     2461 cctatcatct gatagaaggg ggcgggagtt ctgtaagaag gatttggatt gcggaaggtt
     2521 acgccacggc gcttaccatt cattatttga caggagaagc cgtcatggtg gcattttcgt
     2581 cggtcaactt tctttctctg agcagcgttg cccataataa gtatccggga tatcagttaa
     2641 ttattgcagc ggaccgcgat ctgaatggtt caggccagaa cagggctgaa actgctgcaa
     2701 aagcgagtca gtgtgacatt gtgctgccac cggtttttgg tgactggaat gatgcgctta
     2761 cccaatacgg agaggaatcc acccggaagg caattcttga ggctttgaag ccacataacg
     2821 ccagtccttt cgacacaatg agtgaagcag aattcacagc gatgagtgtc agcgaaaaag
     2881 ctcagagggt tcgggagcat tacagggatg cactggcggt tgatccgaac gggcagcttt
     2941 tatcccgcta cgagtctgga gcgtggaaag ttatttctca gtcagatttt gcccgagatg
     3001 tcgcagccct tttccagcgc ctcggggcac cgttttcctc aggaaaaatt gcctcactgg
     3061 tggaaacatt aaagttaatt gttccgcagc agcaaaatcc gtcacgtcat ctgattggtt
     3121 ttcgtaatgg cgttctcgat acgcgaacag ggctttttag tccgcactgt aaagagaact
     3181 ggctgcgtac cctgtgtgaa gtcgatttca cgccaccggt aaagggggaa acgcttgaaa
     3241 ctcatgcccc ggcattctgg cgttggctgg accgggccgc cggacacaag ccagcaaaac
     3301 gcgacattat tcttgcagcg ttatttatgg tgctggcgaa ccgctatgac tggcagctct
     3361 ttctggaagt gactggccct ggtggaagtg gcaaaagtat tctggctgaa attgcgacca
     3421 tgcttgctgg agaggataac gccacctctg cgaccattga aacgctggag tccccgcgag
     3481 aacgtgcggc tctgataggt ttttctctta ttcgcctgcc tgatcaggag aagtggagcg
     3541 gcgacggcgc cgggcttaag gctataactg gtggcgatgc agtatctgtg gaccccaaat
     3601 atcagaatgc ttattcaaca catattccgg cggttatctt ggccgtaaat aataatccta
     3661 tgcgcttcac tgatcgcagt ggtggtgtgt cccgcaggag agttattctg cattttcctg
     3721 agcagatagc accggaagaa cgcgatccgc agttgaaaga taaaattgca cgagaactgg
     3781 ctgtgattgt tcgtcagctc atgcagcgtt tcagcgatcc gatgagcgcc agaacattgc
     3841 ttcagtcgca gcagaattct gatgaggctc tcaccatcaa gcgtgatgct gatccagcat
     3901 ttgatttttg tggctacctt gaggcattac ctgacaccaa cggtatgttt atggggaacg
     3961 ccaatattgt cccacgccag ccacgtacat acctttacca tgcctacctc gtatacatgg
     4021 aggctaatgg ctataaaaac accctgagtc ttacgatgtt tggcaaaggg ttgccagtca
     4081 tgctgaagga gtacgggcta cattatgaga aacggcgaac taatcaggga atgcaaacta
     4141 acctcacttt gaaagaggag agtaatgcag actggctgcc caagtgtgat gagccaatat
     4201 taaaataacg attaactaac ccggctgatg ccgggcttct tattggaaac gcacaaatgt
     4261 agagatagct gttcactctt cacctcgctg ttaacacgta aacaattgat atataagtta
     4321 aaaatggcag ggtgaatagt gtgcatggtt ttgcgaaaaa aaagtatttt ttgtagagtc
     4381 aagtcccggt ttggatccg
//
LOCUS       DAZKGS010000253         4360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKGS010000253 DAZKGS010000000
VERSION     DAZKGS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..173)
                     /locus_tag="ACHOQY_004679"
     CDS             complement(<1..173)
                     /locus_tag="ACHOQY_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680617.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTV"
     gene            complement(163..342)
                     /locus_tag="ACHOQY_004680"
     CDS             complement(163..342)
                     /locus_tag="ACHOQY_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680618.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(518..1069)
                     /gene="ymfL"
                     /locus_tag="ACHOQY_004681"
     CDS             complement(518..1069)
                     /gene="ymfL"
                     /locus_tag="ACHOQY_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP9680619.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(1062..1322)
                     /locus_tag="ACHOQY_004682"
     CDS             complement(1062..1322)
                     /locus_tag="ACHOQY_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680620.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            1420..2112
                     /locus_tag="ACHOQY_004683"
     CDS             1420..2112
                     /locus_tag="ACHOQY_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9680621.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(2432..2947)
                     /locus_tag="ACHOQY_004684"
     CDS             complement(2432..2947)
                     /locus_tag="ACHOQY_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680622.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            3092..3403
                     /locus_tag="ACHOQY_004685"
                     /pseudo
     CDS             3092..3403
                     /locus_tag="ACHOQY_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3418..3780
                     /gene="yfdP"
                     /locus_tag="ACHOQY_004686"
     CDS             3418..3780
                     /gene="yfdP"
                     /locus_tag="ACHOQY_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP9680623.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            3846..>4360
                     /locus_tag="ACHOQY_004687"
     CDS             3846..>4360
                     /locus_tag="ACHOQY_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFP9680624.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISNIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEA"
BASE COUNT         1179 a         1056 c          970 g         1155 t
ORIGIN      
        1 ctgacggtac tcatccccgc gccaatctga cgggcaatag tttcaattga tggccagcac
       61 acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct ggataatttc
      121 atgcctgatg cagcgcaacc atcccataca tagccggtta atttagtgct catgaccgac
      181 ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact catgctcata
      241 gccttcgcgg aggtagataa ctcgttgtgt ttccggctcc caacgaatga ctctgacggg
      301 tactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt ctccttctgc
      361 cggttaaaat cacccacagc ccactctgca aagctgtggg ttacaattac cctgccacct
      421 ggtacattaa ctgcatagca atactccacc ttcgcttttc cacccggtac aggaagcgca
      481 atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt tctccacagt
      541 cacgacacgc cacggcgccc ggagctgcac actcgcgggc gtcattactt tctgaaatgc
      601 aaaaaatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag aggtacagct
      661 cgtcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcaatt gctgaacgaa
      721 tctgttttga ataactgccg atctgttcaa tgacctccag caggcgttgg ttgatatcgg
      781 cgttgtccac atcctcgaca tcaggaagag acacaaagac gccatttgca gactgcgcca
      841 cagcgtcagc aatgaagtga gttccaccag cacgttgcaa aatcattgcc catcccagcg
      901 ggaaaatctg atcgccatcg gcacgaaggc ggttaaataa tgcgttctct gttacatcca
      961 gccagtcagc agcttcagcg tacccccccg gcaacgctgc gatagttttt ctgacagctt
     1021 tcacgtacca ctcaggctgt ttttctactt tccagtgatg cttacccacg gttcacctcc
     1081 tgttcctgtg gtttaaaccc attctggttt tggctagatt gaaaacgtgc cggataaaga
     1141 atctgcattt cgctgacttc acccttaaaa aaattggcta aacgttctgc aagctcgata
     1201 gatggaatct gctccagcct ctcaatacga ctcaacgtcg ctggattgac ttgaacaccc
     1261 gcagcaacat gctgcaaagt gaaaccatgc gccttacgca catttcgtaa tggtgattgc
     1321 atacgtcctc caaatattgc gcgttatgca tgttatttca cgcaattatt ttgcgcaagt
     1381 tgatttgctt atcacgcaat aaagaaatgt aataaacgca tgaacatagg aaaccgagtc
     1441 agacaacttc gccaagcgaa gaacatgaaa atcgccgatc tcgctgaagc gataggagta
     1501 gatgcggcga acatctcgcg cttagaaacg ggtaagcaaa aacaatttac cgaacaaaca
     1561 ctgagtaata ttgccaagag cttaggtgtt gatattgctg atctctttac ctctgcccac
     1621 aaaagtaata ctgtatataa aaacagtaat aatgaggatg ttgcgcaggt gaaggatgtg
     1681 ttccgtattg aaatgctgga tatcagtgcc agtgcgggaa atggccttat ccagggcggt
     1741 gatgtcattg atgtgattca tgccatcgaa tacagaactg ataatgctgt atcaatgttc
     1801 ggcggacgac cagccaatca catcaaagtt atcaacgttc gtggggacag tatgtgtcca
     1861 accattgagc caggagatct catcttcgtt gatgtcagca tcaatcagtt tgatggtgat
     1921 ggtatatatg tctttggttt tgatgacaaa atatacgtta aaagacttca aatgattcct
     1981 gacaaactgc tggtgatttc tgataaccag atttaccgtg aatggggaat tactagcgaa
     2041 aacgaacacc gattcatggt ctttggaaag gtcttaatca gtcagtcgca aacccttaag
     2101 agacataatt aacctcaata tcccatccat cggccaccga aaggtggctt tttattacct
     2161 ataaatttgc atatcacgca aatatcactt gcatatctcg caatttaatt ttatcttttg
     2221 ttccagacca actacaggat tacaacaaaa tctggttgca acacggtgca tgtgtcgtaa
     2281 gcagtcagta aatgtcaaaa acgaacaggc aggacgccca cgaagtagcc gcctggggca
     2341 tatgaagtcc aggatgattc gttagcaaca aaaaagcgcc ctacaggacg cttagctctt
     2401 taacaatctg gatatccaca acagtagtaa tctacagatt gccgttaagt tttctggaca
     2461 actcctcaat ggatggaggc gatacgtaat ccggattttt attcatcaga aacttatttt
     2521 cacagtggag gcacctgctt ttatgaaaaa gctcatcttc gctaaccggg aatggttgaa
     2581 gtatcgatac tatcttttgt ccaaaacatt ttgggcaaag atgcatggtt atgctgccac
     2641 cgttcacgat tacctccttc gagtatacaa aagtacccga ctcaagttgg ttaaggatat
     2701 agccttccgt ctgagcctca aagttttcga attctgcaat tttagctttg agagaagcat
     2761 ttatttcttg ataagagccc accagttcaa cgagagacac gcattcgcgc tgaatagacg
     2821 caagctttga gttcagctca ccaatagccg catttacttc agcttgagtt tttgcctcgt
     2881 tcattagttt tgcaatctgg gctgtttcac gaatagccgt cattgctgcc gttaattcag
     2941 cgatcacatt gaatactctt attgttgttg gggatatcca gattaaccga atccttgttg
     3001 ttggggaata accaggtcca cctcgcctga tgtggctaaa agcaggcaca taacagctaa
     3061 gtattttcaa ccagagagaa tccttagcgt tgtggtgaat gcggctcagc gcacgcgggt
     3121 taaggttgag gctgacagtc gaccttctgt ggatacccac ccgcctggtg tgcaaccttc
     3181 gccaggcacc gggaggcacc cggcaccaca actttatgct gtgtgtagtc ctggcggtac
     3241 cagtttgtac ccttgcttcc ggctggtacc gtcctttttt gcaaaacaga gaagagcatc
     3301 accggacgac gggctcataa cccaatccat ccgggcggct gccaccgcag gtgttcttct
     3361 ctgttttgtg gagaaaccaa ccgaccttgc aaggtcgata tgatgaggag cagcaaaatg
     3421 gctagcgaac gcagtactga tgtgcaggca tttatcgggg agctggacgg cggcgtattt
     3481 gaaaccaaaa tcggcgcagt tctcagtgaa gtcgcttccg gtgtgatgaa cacgaaaacc
     3541 aaagggaagg tctcgctcaa cctggaaatc gaaccgtttg atgagaaccg tgtgaaaatc
     3601 aaacacaaac tctcatatgt tcgcccgact aaccgcggga aaatttccga agaagacacc
     3661 accgaaacgc cgatgtatgt caatcgcggt ggtcgactga ctattctgca ggaagaccag
     3721 ggacaattac tgactcttgc cggtgaacct gacggaaaac tccgcgcagc aggtcattaa
     3781 tatcgttctt aattaactga ttatttatct catcactgaa tatctttata tagtgaggac
     3841 ttattatgtc tcagaactta gacgcaaccg caattaatca aatccatgcc cttatttctg
     3901 ctcagggtgt taatgaaatt atcagtaata ttggtgccga tgctgtggca ttgcctgaga
     3961 atttccgcat tcatgatctg gaaaaattta atttaaatcg cttccgtttc cgtggtgcgc
     4021 tttccactgc cagcatcgat gactttaccc gttattctaa agatcttgca gatgaaggca
     4081 cccgctgctt tatcgatgcc gataatatgc gagccgtcag tgtgcttaac ctgggtacta
     4141 ttgatgaacc aggtcacgca gataacaccg ccaccctcaa actgaaaaag acagcaccgt
     4201 tttctgctct gttgtctgtt aatggcgagc gtaactccca gaagtcactg gcagaatgga
     4261 ttgaagactg ggccgactac cttgtgggct ttgatgctaa tggtgacacc attcaggcaa
     4321 ccaaagcggc tgcggcagtc cgtaaaatca caattgaagc
//
LOCUS       DAZKGS010000254         4347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKGS010000254 DAZKGS010000000
VERSION     DAZKGS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..476
                     /locus_tag="ACHOQY_004688"
     CDS             114..476
                     /locus_tag="ACHOQY_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680625.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            492..620
                     /locus_tag="ACHOQY_004689"
     CDS             492..620
                     /locus_tag="ACHOQY_004689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680626.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
     gene            886..1105
                     /locus_tag="ACHOQY_004690"
                     /pseudo
     CDS             886..1105
                     /locus_tag="ACHOQY_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1083..1355
                     /locus_tag="ACHOQY_004691"
     CDS             1083..1355
                     /locus_tag="ACHOQY_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9680627.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(1379..1918)
                     /locus_tag="ACHOQY_004692"
     CDS             complement(1379..1918)
                     /locus_tag="ACHOQY_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFP9680628.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            2174..3142
                     /locus_tag="ACHOQY_004693"
                     /pseudo
     CDS             2174..3142
                     /locus_tag="ACHOQY_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            3167..3298
                     /locus_tag="ACHOQY_004694"
     CDS             3167..3298
                     /locus_tag="ACHOQY_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP9680629.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            3283..3435
                     /gene="kil"
                     /locus_tag="ACHOQY_004695"
     CDS             3283..3435
                     /gene="kil"
                     /locus_tag="ACHOQY_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9680630.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            3432..3590
                     /locus_tag="ACHOQY_004696"
     CDS             3432..3590
                     /locus_tag="ACHOQY_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680631.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            3692..4297
                     /locus_tag="ACHOQY_004697"
     CDS             3692..4297
                     /locus_tag="ACHOQY_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFP9680632.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
BASE COUNT         1443 a          930 c          982 g          992 t
ORIGIN      
        1 agatatttgt gcttaacatc aattaatcat tgctaaatcg tgcttattgt tcagaaatcg
       61 cccaagtcat attgaaaatc tgttggcgaa cacctatata aggagtatag ttagtgaccc
      121 aaaacgtatt gccaatcaac aagagcttgc atgaccgagc tgttgatgaa tttaatcgtc
      181 tgcatggaac aatgattggc gagattagtg caatgctgaa aacagctaaa gtagcgccat
      241 tggtggatct tcgtaagaaa gatcctacct tttcgaacgt tgtcgcagaa ttacggacat
      301 tcagagacgt atgtaatgct ctgctcccat actttcgtgt agataagacc agcgaaatcg
      361 ctgtcatcga taagttactt atactggcta atgacctggc tcaagccatc gatgctgacg
      421 atcctgacgc attgtgcgca gcaatagcag cccttgatgt tgagccttac atttaaacga
      481 ggggaaaaaa aatgactaaa gcgtttgact atactgccgt tagtaagctt ttggctgaaa
      541 tgcgcggatg tgtagagcgc gttcaaaatc tacgccgcga ctttgaagct cacatcaatc
      601 attcacaaaa agcggcttga tacttaagtt agatgacaga acccggcctc agcgccgggt
      661 tttctttgcc tcacgatccc cacacctaat aacaccttaa ccaattgtat ttattgaaaa
      721 attgatagat acaactttct aaaccacgaa attctgatcc ccctcaagtc tcttcattcc
      781 ttctgataaa ttcccgcaat caaataaaaa accataataa tcaacaggat atttgaaaac
      841 ccaaacaatt tatcattttg ctattgccat cagtttatca tttcgataaa gtcaactcat
      901 cagcaggacg ctgtaagcca aacggagcag actggcaggc tctttaaaca acgtcgaaca
      961 ctcgactacg tggctgaaaa gccagatcac ccaaccacat aagctgtggg atgcaatgcc
     1021 gaagtaaccg tctcaggagg agcttcgaga ttgcatcgcc aaagtttatt cgggaggaat
     1081 ctatgtccag aaaaacagaa tttaaaggca ccgcagcttc tcgccgtaga gctcgtcgcg
     1141 caaacctgca aagtcaggag gcgattagct ccgacaagct acacaggcca accccttcac
     1201 gagtggtctt gcaatgcaag cgcaaaccag caatgagagc agaagttata actctgacaa
     1261 cgttgaccag aaaatatgaa ggttcaactt gtcttccaaa cgtagccatt tacgcggcag
     1321 gctaccggaa atcaaaacaa ctgacagcga ggtgacgatc ccccccgccg tagaggaact
     1381 aaattagctc atcagcttta ccagcaaagc gcatataaca ctcatggaac cagacgtcag
     1441 gtataacaaa tttctcttta cctgacgcgc tgtaagtcac acctgagcga tggagaaggc
     1501 cttttctcat aagagataaa gcgaccggat cgccgtgctt aagtacgatt ttgttatttg
     1561 ccataacagc aaatgctaaa acctcttttt cctgaatact cagagaattg aataggcgct
     1621 cagtctccac aattacttta gcctcatctc tcttactgag atatttttta tatctgtctg
     1681 aagatgccag aaatataaac tccatccccc tgttcagaac ataactgata cacaaaagca
     1741 ttgcgtaata catccagtga tctggaagta tttcggggtt ccttatatct acccactctt
     1801 taattgatgc tggggtgatt attatcaagg caattaaaat aataagcata tgaattaact
     1861 gttgtagcgt aagaccacgc aggaagaaac gtaatagttc ctgccaccag ttactcatcg
     1921 gtgaatctcc atcattctct ctgtaggggt gaatagagtt tatccgattt ctcgctgtag
     1981 gggtacacga gaaccaccga gcctgatgtg gttaaaagac aggcatacga ataaacactg
     2041 cactgtgtat tcattccaac gagtgaatac acggagcaat gtcgctcgta actaaacagg
     2101 agccgacttg ttctgattat tggaaatctt ctttgccctc cagtgtgagg gcaatttttt
     2161 tgacggagga tatatgagtg aagtaacaga tttagttgtt attgaaaaag caaatgcaat
     2221 gactgtaatt tagtctgccg accagattga agaaattctt caaaaggttg aacgtgaagt
     2281 tatgtccttt gtgcctgata tcacaacggc aaagggcaga aaggagatcg cttatctggc
     2341 gtataaagtt gcgcagacga aaacatatct cgatggtctt ggcaaagacc ttgttgctga
     2401 actgaaggaa attccaaagc taattgatgc taaccgcaag acagtgcgcg atcgccttga
     2461 tgaactgaaa gccaaggcgc gccagcctct tactgattat gaggaagaac aggcgcggat
     2521 taaagccgaa gaagaagcta aggcagcagc tgaagctctc gcaaagcaaa ttgagtctga
     2581 ccatgaaata gcgattttga tggatcgcga atttgaccgc caaagagaag aggcaagact
     2641 caaagcggag caggaaaagc gagagcatga agaacgctta aaaagagaag ctgaagagaa
     2701 agccagagct gaagccgaag caaaggcaaa agccgaaatt gaagcagcag caaggcgaga
     2761 agcagaagct aaggccgcag aggaacgtgc agagcgtgaa cgcattgaag ccgagcaacg
     2821 agcacagcgc gaagcaaaag aggcagcaga acgagctgaa agagaaaagc atgcggcaat
     2881 tgaagcagaa cgccgtaaag cacaggagga ggcagaacga atcctgcgcg aggctgaagc
     2941 aaaagagcaa gccagaatag cagaagaaaa aagaatcaag gacgaagaag agcgtagagc
     3001 aaaggataaa gctcaccgga aagaagtaaa taacaaaata cttgctgacc ttatcaaggt
     3061 tggcgcatca gaagatgttg ctaaaaatat cataacagcc atcgtaaaag gcgaattatt
     3121 cgcaacaaaa ataacctact aataaaacca acataaggaa ccacccatga tttacgcaat
     3181 cgcgggaggc gctcgcatgg gtgccttcca attaaatgaa tctttacttg aacgaatcac
     3241 ccgtaaatta cgtgacggat ggaaaagagt tgaggtctta ttatgcgcaa tgaaatagcc
     3301 atcaatcacc agatgcttcg tgctgcacag aacaaggcag taatagcccg atttattggt
     3361 gattcaaaaa tgtggcttga agcaaataaa gcgatgaaat cagctatcaa ccttccgtgt
     3421 tatcgcagga aatgagtttt acagataact ggtcagacga agaattcatt cgtcagatga
     3481 aagaattaat cggtaacgaa ggagatattc atgtcacttg caaccacagt gaaggagagc
     3541 aagttacaga ggcgcatgta cacgcagaaa gctctctggt atcgccataa tggtgaccgc
     3601 gaaggaatgc gggtatgcct taatttgtcc cgagtcgaag tattaaatca gcgttatttc
     3661 cttgggccgt gtccattctg agaacaatca tatgagcaaa gaattttacg caagactggc
     3721 agctattcag gagaatctga acgcgccaaa gaatcagtac aactcattcg gcaaatataa
     3781 atacagaagc tgcgaagata ttcttgaagg cgttaagccg ttactgaatg gcctgttttt
     3841 atcaatcagc gatgaagttg tgttgattgg tgatcggtat tatgtgaaag ccacggcaac
     3901 tattaccgat ggcgaaaaca gtcatacggc aaccgctctt gcacgagagg aagaaagcaa
     3961 gaaaggaatg gattctgcac aagttacggg agctacaagc tcttatgcac gcaagtattg
     4021 cctcaatggt ttattcggca ttgatgatgc gaaagatgca gataccgacg agcataaaca
     4081 tcagcagaac gcagcagcaa agcaatcaaa accatcacct acacctgaac aggttctaaa
     4141 agcattcact gacgcagcaa tgcagaaaaa caccgtagaa gagcttaaac aggcgttcgc
     4201 caaagcgtgg aagatgctcg aaggcacacc ggagcagcac aaagcgcagg acgtttacaa
     4261 catcagacga gacgaattag aaggagctgc tgcttaatgg cacattcgat tactgtaaga
     4321 ctaaacaagc ccgcaagaga gtttcag
//
LOCUS       DAZKGS010000255         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKGS010000255 DAZKGS010000000
VERSION     DAZKGS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            288..896
                     /locus_tag="ACHOQY_004698"
     CDS             288..896
                     /locus_tag="ACHOQY_004698"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9680633.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="ACHOQY_004699"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="ACHOQY_004699"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9680634.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..4226
                     /gene="selB"
                     /locus_tag="ACHOQY_004700"
     CDS             2382..4226
                     /gene="selB"
                     /locus_tag="ACHOQY_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9680635.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          976 a         1073 c         1237 g          996 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgttggttt gattcgccga
      481 tcatcgctga atatattgaa ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cactggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccag cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgcc gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggctggt gtgtcgatcg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg gcagccagac gctgcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaagc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg gcgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgctgg acgcggacat
     1381 cgcgatcgtg cgctggcgca gttgctgtgc cgcattacgg gggcggaaga tgcctgtatc
     1441 gttaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtggttt ctcgcggcga actggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattattgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agtttctccg gcgacaagtt gttaggcggg ccgcaggcag gaattattgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ccccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccggtgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggcgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct ggttttatcg acgttcccgg tcatgaaaaa tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gtgtgatggc acagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggt gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac
     2761 gtcaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgagga attgatgccc tgcgcgagca tctgcttcag ttaccagaac
     2881 gtgaacacgc cagccaacat agtttccgcc tcgcgatcga ccgcgcattt accgtaaaag
     2941 gtgccgggct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tatggctaac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc
     3061 caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggttat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt atttccggaa aaataagaaa atgattaatt aatttttaaa ataaattaat
     4261 acaaatttct tatgatttta aa
//
LOCUS       DAZKGS010000256         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKGS010000256 DAZKGS010000000
VERSION     DAZKGS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..377)
                     /locus_tag="ACHOQY_004701"
     CDS             complement(<1..377)
                     /locus_tag="ACHOQY_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP9680636.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVYFVENES
                     WGSVIYNLFLQLEKENKSHTSLEV"
     gene            complement(368..931)
                     /locus_tag="ACHOQY_004702"
                     /pseudo
     CDS             complement(368..931)
                     /locus_tag="ACHOQY_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1165..1842)
                     /locus_tag="ACHOQY_004703"
     CDS             complement(1165..1842)
                     /locus_tag="ACHOQY_004703"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9680637.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            2040..3659
                     /locus_tag="ACHOQY_004704"
     CDS             2040..3659
                     /locus_tag="ACHOQY_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFP9680638.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            3656..>4282
                     /locus_tag="ACHOQY_004705"
     CDS             3656..>4282
                     /locus_tag="ACHOQY_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFP9680639.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLAGRVYDPAVQR
                     PLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIR
                     TVREKHACTQCDAIVQAPAPSRPIERGIARPGLLARVLTSKYAEHTPLY"
BASE COUNT         1057 a         1081 c         1021 g         1123 t
ORIGIN      
        1 acttctaatg atgtgtgaga tttattctct ttctcaagtt gaagaaaaag gttatatata
       61 acgcttcccc aagattcatt ttcaacaaaa tacacatcac ccttaatatt atttgtcccc
      121 ttgttttcct gaattacaat agctttgctt aataattgag gggatgaaaa tgtttcgtag
      181 tctatctttc ctgaattact acaaaaaaac tcactccaat atgatgctat atgtcgacac
      241 catatgattt ccccattgca atcactaacc tttccattta gatttatact ggcatctctc
      301 ttttttgata aataaggggg attgtttttt gaaatatatt caacataatt tgatgccgat
      361 ttgtttatta ttggcatatg tttgtatcct ttctataaat tttccgcctg acagtggaaa
      421 taatatattc tactgatttt tgtagtttaa acgatttatg aagaatttta ctgttgtaca
      481 gtaccagata ctcagccagt ttcagattga acatattcag ctattcaaag agcaattctt
      541 cattaaactc aataaattgt tccctcagtg ttcggttaaa atgctcactt atggcattta
      601 ttttcggcgt atagagataa gtccatacga acccgacggt ggcttcctgt agcgttttgt
      661 cgaattttcc cgagaactct ttgcccatca tcagtgatga tctggttgat accaacaagg
      721 aacaactggg ctacacagca gaagaaatgc ctgatgatat cacttttaag cgatagtacg
      781 gccagagagt agttgctgtg ttcgtcgagc atggtaatga aataacggta cagctcgcca
      841 ttctgagctc aatagtatcc atgccactga gcttacaggt ctttatcggg cgatagtgct
      901 ttattcttcg gggctttgtg gatgattttt cgaaatgttc atgatagtta ggtaaagcta
      961 ccgcatatca tttgcaaaat gacagataga tatacttcaa tttgaatctt aacaattagt
     1021 atgggaatgc tgtcaatcct ctgtatgcgt ccaccacata ttgaaatttt tgttctctaa
     1081 tatattccac gggcgtcaag agtgtgaggc ctgtgctgtt atacagcacc agacgttgtc
     1141 ccccatcttt gacctgtctc ttgatcagat ctcctgatca agagacttca tcaccaggta
     1201 accctcagcc atatcctgat gtctgaacca gccatcccac ataactaccc aaccggggct
     1261 gcttgtgcgt ttgctgtcat gccatcgccc cagtttagcc agtttcaaac aggcccattt
     1321 cagggtcggt gtttgcgctg gaagctgctt tccttccagc ttaacccaca gtagtttcca
     1381 ctctgtcggc gtcagtattt tctcacagct gtcattttgt gtttcttcac tgatacttcc
     1441 ctgccgcagg cccagcaccc gcaccgcgat aaccaccatg cgctcaaggt tatcccgggt
     1501 ctgcattcgc agcgattcca cacatgtacc accacttttc cacgctttgt ggtattcctc
     1561 tatcagccag cgttgctcgt aatggctgac gatacgtcgc gcatcggcgg cactcgccac
     1621 tttttctgat gtcagcggat accagcaggc tccgtcctct cctgctcccc gcaacagaca
     1681 tacgtgagcg agagcgcctg gccgctgttg tcgggatttt ttatgctgac ttcgctgtaa
     1741 ctgatgagca tccgggcctg gcgggctgcc cgcccgcctt tttgcatcac attcagcgtg
     1801 tggcttcccg cggctgactt tataatgcag gtaatgccag atatccgctt cccggtcaca
     1861 gacagtgatt acctgtttct gtatctcccc cagccgttcg gccatgcgct ccgaagcctg
     1921 ctgccagcgg taactttctt tttctgtcac gaacggtgca atagtggttc ttccgcaact
     1981 tgggtggaag aaaatggatg aaactttctg gtgaacaaag atctaagctg ttttaagtca
     2041 tgggcaactc aatgcactct cttaagacac ttctacagtt accttgcgga tggcgatgca
     2101 gtcgacaaat tattagctct gacggtataa ccctccatct ccacggaaaa cgcaaaaaag
     2161 cacaatgtcc tgaatgcttt aagcgtagcg actctgttca tagttgtcgt cggcgccgga
     2221 tacagcatct accctgctca ggacatacgc tatggcttgt attttccgtc cgccactggt
     2281 actgccgtaa cccggcttgt tcacgtaaaa tttttgccgg gtcgcttgct cccttcgccg
     2341 gttcacacca acagtcttca caggcgttac aaaatttaca acgtcaactg ggattaatcg
     2401 ccggaggtga ggccgggaaa cgggcagcaa cggcagtggg gctccgttgc agtgctgata
     2461 ctctccttcg cagggttatc aataccccgg agacgaaaca gtcaggcgcg cctcatgtcg
     2521 gtattgatga gtgggcgtgg catcggggcc accgttacgg tacgttaatc gtcaatcttg
     2581 atactcaccg tcccctcgtc ctgcttcccg gccgtgatca gcgtacgctg gcgacctggt
     2641 tcagaaaata tccggaaata caaattgtct cgcgtgatcg cagtggagtc tatgcgacag
     2701 cagcacgtga aggtgcacct caggccagac aggtggccga tcgatggcat ctgctaaaaa
     2761 atattggcga tgcgcttgaa cgaatgatgt acagacatat acctctgata cgtcttgttg
     2821 ccagtgagtt gtcaccaaag aaatcacctg atccagaacc atctgtgcct gcagcatcgc
     2881 tccgtcgtcc ggaacgccta aaacaacaaa cccgcaaaaa acggcatcag cgttggacag
     2941 aggttatggc cctgcataac aaggaatgta gtttcaggga aatatcccgt attacaggcc
     3001 tgtcgcgtgt gacagtcagt cgctgggtgc gttcaggaac attccctgaa atgtcaaccc
     3061 gacctccaaa gcgagggctt ctggacccat ggagggagtg gttaaaagag caacgagaaa
     3121 gtagtaatta taacgccagc cggatatggc gggaaatggt ggcccgggga tttacaggca
     3181 gtgaaaccat cgtcagggat gcggttgcca aatggcgtaa aggctggaac ccaccggtta
     3241 ctactgccgt cagacttcct tcagtgtccc gggtaagccg ctggttgatg ccctggagaa
     3301 taaccaggga tgaagaaaat tatgcttccc gatttattag tctgatgtgt gaaaaagaac
     3361 ctgagctgaa aatagcgcag caactggcac tcgagttcta ccgtattctg aaaacccaaa
     3421 ataaatcaca gctaagcagt tggttcaatc gagtccacga aagcggttca gcagaatttc
     3481 ggcgcgtggc ggcggggatg gaagctgatg ctgcagctat atgtgaggca atcagtagtc
     3541 gctggagtaa tggtgttgtc gaaggtcatg taaatcgcct gaagatgctg aaacgccaga
     3601 tgtatggtcg agcaggattt gaactactca ggcagagggt aatgagtcca ctggcatgaa
     3661 aatctccacc caggttgcgg aagagccact ttccgtcgtt gacactgacg acatcttcct
     3721 gctgaaacag cgcctggccg aacaggaagc gctgatccac gccctgcagg aaaagctgag
     3781 caaccgggag cgcgaaatag accatctgca ggcgcagctg gataaactcc gccggatgaa
     3841 cttcggcagt cgttccgaaa aagtctcccg ccgtatcgca caaatggaag ccgatctgaa
     3901 ccggcttcat aaagagagcg atacgctggc tggtagggtg tatgacccgg cagtacagcg
     3961 tccgttgcgt cagacccgca cccgtaagcc gttccctgaa tcactacccc gtgacgaaaa
     4021 gcgactgttg cctgcggcgc cgtgctgccc gaactgcggc ggttcactga gctatctggg
     4081 cgaggatacc accgaacagc tggagttgat gcgtagcgcc tttcgggtta tccggacggt
     4141 acgggaaaaa catgcctgta ctcagtgcga tgccatcgtg caggcacctg caccttcgcg
     4201 gcccatcgag cggggtatcg ccagaccggg gctgctggcc cgtgtgctga cctcgaagta
     4261 tgcagagcac accccgctgt at
//
LOCUS       DAZKGS010000257         4274 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKGS010000257 DAZKGS010000000
VERSION     DAZKGS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..445
                     /locus_tag="ACHOQY_004706"
     CDS             <1..445
                     /locus_tag="ACHOQY_004706"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9680640.1"
                     /translation="MPVVQFLNITLAIALLFLCLGLRNGPGIVAWGAYSFMIIAPIVL
                     ISKCRDVYRNKILTYVGEISYSLYINHMVVYSLAVLYLKPYGVLDSKGFGVFLAMICA
                     TFIISIISYNLIEKPSVSIGHAIARRMQKKSDVSSIKTESVREAP"
     gene            complement(690..1847)
                     /gene="intS"
                     /locus_tag="ACHOQY_004707"
     CDS             complement(690..1847)
                     /gene="intS"
                     /locus_tag="ACHOQY_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFP9680641.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2009..2083)
                     /locus_tag="ACHOQY_004708"
     tRNA            complement(2009..2083)
                     /locus_tag="ACHOQY_004708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2048..2050),aa:Arg,seq:cct)
     gene            complement(2159..3091)
                     /gene="yfdC"
                     /locus_tag="ACHOQY_004709"
     CDS             complement(2159..3091)
                     /gene="yfdC"
                     /locus_tag="ACHOQY_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9680642.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3385..4140
                     /gene="mlaA"
                     /locus_tag="ACHOQY_004710"
     CDS             3385..4140
                     /gene="mlaA"
                     /locus_tag="ACHOQY_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9680643.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1059 a          995 c          996 g         1224 t
ORIGIN      
        1 aatgcctgtt gttcaatttc ttaatattac attagcgatt gccttgcttt ttttatgtct
       61 agggttaagg aatggacctg gaattgtagc atggggggct tattcattta tgattatagc
      121 cccaatagtc cttatttcta agtgtaggga tgtatataga aataaaattc taacgtatgt
      181 tggtgaaata tcctattcac tttacattaa tcatatggtt gtatacagtc ttgctgtttt
      241 atatctcaaa ccgtacgggg tattggatag taaaggattt ggtgtttttc tagctatgat
      301 ttgcgctact tttattatat caatcatatc ttataacctt attgaaaaac catcagtatc
      361 catagggcat gccatcgctc gtagaatgca aaaaaaatca gatgtatctt caattaaaac
      421 tgaatccgtt agagaagcac cttgataaat agagcaatag ccagatagca cctaaccgat
      481 ttcgtactca tagggttgtc actttaccaa actgagagga aatacactga taacagttgg
      541 ttatgaagta ctgggtgagg tggatgtgaa tggtattttg atgcatttca ttaacctgta
      601 cgcctaagaa agcaaccctg aaatctaaga cacacaagct ttgcactgga ttgcaaggct
      661 ttgtgcactt cgatagtggt taaggtggtt cactccacct tttcatcaag ccagtccgcc
      721 caccattgca tcatttctct gcgcttatcg agatactgag catggttgta aataccgcgc
      781 acagatccgc cgttggcatg tgccagttgc acttcaatag cgtcagcagg ccattcgtgc
      841 tcgttcataa tcgtgctgaa ttcatgcctg aatccgtgac cgctttccag accctcatag
      901 ccgatttgtt tgatcacaag tagcaccgcg ttctcgcaga ttggcttctt cttatcgttg
      961 cgcccggcaa aaacaaattc tgatactggt ttggtgatgg agcttaacgt agtgagaagt
     1021 tcaaccacct ggtccgacat cgggacaaca tgaattttgc gtcccttcat cacattggcg
     1081 tcaatggtga taatcctgtt ttcaaaatcg acgttcttcc attgcatgga acgaagctct
     1141 tttgttctta gggctgtgta gcgtaaaacc ttggtggcaa tgagcgatac gatacttcct
     1201 gaaaatgttg ccagtgcttt attgaatgcc gggatctggt ctgcaggaag aaacgggaag
     1261 ttcttcttgc ggtatcccct catggcgtca gcaaggtcag gtgccgggtt atatttagcc
     1321 ctgccggtga caatagcgta acggaaaacc tcgccgcatc ttctgcgggc tttatttgct
     1381 cgctccattg caccgcgatc ttcaaatctg cggattactt ccagcagttg catcggctca
     1441 atatcctgaa tttcaaggcc gccgatgata ggcaaaatgt cgtcatcaaa catttttgca
     1501 agttcagtcg cataccctac tgaccatact tgcttcttgt gctcgtacca ttccttgtaa
     1561 atcgcactaa aggaattgtt gttagacgaa gcctttttcg cctttaccgg atcgatgcca
     1621 accgagatgt ctttcctcgc agtccatgcc ttatcccttg cctcctgcaa agtcattagc
     1681 ggatattttc cgacggtcag gattttctcc ttaccgtcaa tcttgtagcg aagctgccat
     1741 acctttttcc cggatacagg aacataaagg tacaggccat taccatcgag aaggcggtat
     1801 ggtttttctt tcggctttgc tgcttcaatc tgcttaacgg tgagcatggg taaaaatccg
     1861 gagggtaaaa ttattttatc cactttttac ccgtcatggg gtgcggctgt caacgatctg
     1921 acgcgaacca tgacgaactg tgaatctacg gaaggcttga tattcagggg attttgcgga
     1981 ctggtacgga tgggagcgaa ctgataaatg gtgtcccctg caggaatcga acctgcaatt
     2041 agcccttagg aggggctcgt tatatccatt taactaagag gacaatgcgg catgagtata
     2101 cccgctaatg gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt
     2161 atgctgggtt tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt
     2221 ctgctttacg cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga
     2281 taaaggtgcc gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat
     2341 cgctccagtg cagcgtgccg ttaaacacca gatagaggat ttcaacagaa ccgacaacga
     2401 tgtgggtggt gtcacccagt gcaataagcc aggtcatcaa aataatcacc acaatctttg
     2461 ccgcacccgc tgcaggaaac atccaaacca tagtggcgat cagccagccg gaaatgatcg
     2521 cgttggcaaa catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg
     2581 catcgcgagt ttcttcattg aagataggca tatattcaaa tgcccatgca gcaatacctg
     2641 tcccgagaat attacccagc agcacgacgc cccataatcg cataagtaag ccgacgttgc
     2701 tcattgtcgg tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt
     2761 gctggcgggc cataatgacg ataataaaac caaaggtata accgagattc tccagtaaga
     2821 agctgcctgg cactccttcc agttcgacat gaaatatccc ttttgccagt agcgaagcgc
     2881 ccatcgacag acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttccagct
     2941 ctttttcacc atcctggcgg atatgctcat gaattgccat cgcccgggag gggagtcggt
     3001 cttcatctat ttctattttt ttgccgcgct ctttttcttc gctctcaact tcaatttcgt
     3061 cgctgtgttg atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag
     3121 ctaaagcgta gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa
     3181 ggctgcgttt gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc
     3241 aggctatgat caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat
     3301 cgcccacacc taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag
     3361 ccataaaaaa acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact
     3421 acgcttctgg tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta
     3481 gaagggttca accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt
     3541 cgaccggtcg ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc
     3601 aactttactg gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac
     3661 ccttatcagg ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc
     3721 ggctttattg atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc
     3781 ttcggtagta cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc
     3841 tacggtagct tcacgctgcg tgatgacggt ggtgatatgg cggatggtct ttacccggtt
     3901 ctttcctggc tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc
     3961 cgcgctcagc tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg
     4021 gtgcgcgaag cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg
     4081 caggaaaatc cgaacgcaca agcgattcag gatgatttaa aagatattga ttctgaataa
     4141 gaaacaaata aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac
     4201 cacaagtaaa aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg
     4261 ttcgtcactg acta
//
LOCUS       DAZKGS010000258         4262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKGS010000258 DAZKGS010000000
VERSION     DAZKGS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(204..1853)
                     /gene="pgi"
                     /locus_tag="ACHOQY_004711"
     CDS             complement(204..1853)
                     /gene="pgi"
                     /locus_tag="ACHOQY_004711"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9680644.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      2090..2286
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2378..3727
                     /gene="lysC"
                     /locus_tag="ACHOQY_004712"
     CDS             2378..3727
                     /gene="lysC"
                     /locus_tag="ACHOQY_004712"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9680645.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3872..>4262
                     /locus_tag="ACHOQY_004713"
     CDS             3872..>4262
                     /locus_tag="ACHOQY_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9680646.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKR"
BASE COUNT         1015 a         1070 c         1090 g         1087 t
ORIGIN      
        1 ggatttatct gataaaaaaa tgccgacata acgtcggcat tgttattaag gttatttaat
       61 caatcgtgct atgtattaat tgccgaatac gatgtacata tcggcatcga cctgtaggcc
      121 tgataagacg cgaccgcgtc gcatcaggca tcggttgccg gatgcggcgt gaacgcctta
      181 tccggcctac atatcgacga tgattaaccg cgccacgctt tatagcggtt aatcagacca
      241 ttggtcgaac tatcgtggct gctgatttct ttgccatctt tcagctctgg cagaatacgg
      301 ttcgccagct gtttacccag ctccacgccc cactggtcga aggtgaagat gttcagaatc
      361 acgccctgag taaagatttt gtgctcatac agcgcaatca acgcacccag gctgaacgga
      421 gtgatttcac gcagcaggat ggagttggtc gggcggttac cttcgaacac tttgaacggc
      481 accacgtagt caagcgttgc cggatcttta ccctgatcgc gatattcctg ctcaaccact
      541 tcgcgggatt taccaaacgc cagcgcttcg gtctgggcga agaagttaga cagcagtttc
      601 tggtgatgat cagagagcgg gttatgggtg atagccggag cgatgaaatc gcacggtacc
      661 attttggttc cctggtggat cagctggtag aacgcgtgct gaccgttagt gcctggttca
      721 ccccagataa tcgggccagt ctggtaatcc acaacgttac cgttacggtc aacatactta
      781 ccgttggact ccatattgcc ctgctggaag tacgccgcga aacggtgcat atactggtca
      841 tacggcagaa tcgcctcagt ttccgcacca aagaaattgt tgtaccagat gccaaccagc
      901 gccagcagta caggcaggtt tttctcggca ggcgtggtgg agaaatgctt gtccatcgcg
      961 tgtgcgccag aaagcagttc aacgaagtta tcaaagccga tggagagaac aatcgacagg
     1021 ccaatcgctg accacaaaga gtaacggccg ccgacccagt cccagaactc gaacatgttg
     1081 gcagtatcaa taccaaactc gccaacggct ttggcattgg ttgagagcgc cgcaaagtgt
     1141 ttcgcaacgt gtttttcatc acctgccgct ttcaggaacc agtcacgcgc gctatgggcg
     1201 ttggtcatgg tttcctgagt ggtgaaggtt ttagatgcta ccaggaacag cgtggtttcc
     1261 gggtttactt ttttcagcac ttcagcgatg tgagtcccat cgacgttaga aacaaagtgc
     1321 atgttcaggt gatttttgta cggacgcagt gcttcggtca ccatgtacgg gccgaggtca
     1381 gaaccgccga tcccgatgtt cactacgtca gtgattgctt tgccggtata acccttccac
     1441 tcaccggaga taatcgcttc cgagaaggtt ttcatcttct ccagcaccgc gttgacttcc
     1501 ggcattacgt ctttgccatc aaccaaaatc ggggtattgc tacggttacg cagcgctacg
     1561 tgcagcacag cgcggttttc agtgcggttg atcttctcgc cagagaacat cgacttaatc
     1621 gcgcccgcca gatcgcactc tttcgccaga tcttgtaatt tcgccagcgt ctcttcagtg
     1681 atgcggtttt tggagtaatc caccagcatc tgatcgccga aggttgcgga gaacttagaa
     1741 aaacgatcgc cgtctttagc aaaaagatcg gcgatcgtaa cgtctttcat ttcatcgaag
     1801 tgtttctgta gtgcctgcca ggcagcggtc tgcgttggat tgatgttttt cattagcaat
     1861 actcttctga ttttgagaat tgtgactttg gaagattgta gcgccagtca cagaaaaatg
     1921 tgatggtttt agtgccgtta gcgtaatgtt gagtgtaaac ccttagcgca gtgaagcatt
     1981 tattagctga actactgacc gccaggagtg gatgaaaaat ccgcatgacc ccgtcgttga
     2041 caaccgcccc gctcaccctt tatttataaa tgtactacct gcgctagcgc aggccagaag
     2101 aggcgcgttg cccaagtaac ggtgttggag gagccagtcc tgtgataaca cctgaggggg
     2161 tgcatcgccg aggtgattga acggctggcc acgttcatca tcggctacag gggctgaatc
     2221 ccctgggttg tcaccagaag cgttcgcagt cgggcgtttc gcaagtggtg gagcacttct
     2281 gggtgaaaat agtagcgaag tatcgctctg cgcccacccg tcttccgctc ttcccttgtg
     2341 ccaaggctga aaatggatcc cctgacacga ggtagttatg tctgaaattg ttgtctccaa
     2401 atttggcggt accagcgtag ctgattttga cgccatgaac cgcagcgctg atattgtgct
     2461 ttctgatgcc aacgtgcgtt tagttgtcct ctcggcttct gctggtatca ctaatctgct
     2521 ggtcgcttta gctgaaggac tggaacctgg cgagcgattc gaaaaactcg acgctatccg
     2581 caacatccag tttgccattc tggaacgtct gcgttacccg aacgttatcc gtgaagagat
     2641 tgaacgtctg ctggagaaca ttactgttct ggcagaagcg gcggcgctgg caacgtctcc
     2701 ggcgctgaca gatgagctgg tcagccacgg cgagctgatg tcgaccctgc tgtttgttga
     2761 gatcctgcgc gaacgcgatg ttcaggcaca gtggtttgat gtacgtaaag tgatgcgtac
     2821 caacgaccga tttggtcgtg cagagccaga tatagccgcg ctggcggaac tggccgcgct
     2881 gcagctgctc ccacgtctca atgaaggctt agtgatcacc cagggattta tcggtagcga
     2941 aaataaaggt cgtacaacga cgcttggccg tggaggcagc gattatacgg cagccttgct
     3001 ggcggaggct ttacacgcat ctcgtgttga tatctggacc gacgtcccgg gcatctacac
     3061 caccgatcca cgcgtagttt ccgcagcaaa acgcattgat gaaatcgcgt ttgccgaagc
     3121 ggcagagatg gcaacttttg gtgcaaaagt actgcatccg gcaacgttgc tacccgcagt
     3181 acgcagcgat atcccggtct ttgtcggctc cagcaaagac ccacgcgcag gtggtacgct
     3241 ggtgtgcaat aaaactgaaa atccgccgct gttccgcgcg ctggcgcttc gtcgcaatca
     3301 gactctgctc actttgcaca gcctgaatat gctgcattct cgcggcttcc tcgcggaagt
     3361 tttcggcatc ctcgcgcggc ataatatttc ggtagactta atcaccacgt cagaagtgag
     3421 cgtggcatta acccttgata ccaccggttc aacctccact ggcgatacgt tgctgacgca
     3481 atctctgctg atggagcttt ccgcactgtg ccgggtggaa gtagaagaag gtctggcgct
     3541 ggtcgcgttg attggcaatg acctgtcaaa agcctgcggc gttggcaaag aggtattcgg
     3601 cgtactggaa ccgttcaaca ttcgcatgat ttgttacggc gcatccagcc ataacctgtg
     3661 cttcctggtg cccggcgaag atgccgagca ggtggtgcag aagctgcatt ttaatttgtt
     3721 tgagtaatat ttccctactg gcgtataacg ctcaccgatt gttatacgcc atcctcactg
     3781 aaaacataga ttaaataata tctgaaaagc aaaatcactc ctgtagaaca gtattataat
     3841 caccgaaaaa tatcatttaa caggagtgtt aatggctact ttaatggaaa aagatgcact
     3901 tttaaacgga gccagtcagt gcatcgcgtt tttatcaaat atcattgata attgctcagt
     3961 ttcgtcgcat caggattcag gcgatgctct caagcgatta gtcagctatc gcgattatct
     4021 ttattctacc cccgctgaac ttgttgattt tacacaaggt aagattctcc ttcagcaggt
     4081 aagaactcaa tatcagcatg aattcaacaa tactacgcat tcagaaaata aagcatcttt
     4141 tgattctatt tggcagcgtc tgacaaatca tgaagtcaca ccgcaacagc atcccattgg
     4201 ttttgtttta ggtgggcaac ccggagcagg taaatcatct ctgattgaat tagccaaaag
     4261 ag
//
LOCUS       DAZKGS010000259         4169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKGS010000259 DAZKGS010000000
VERSION     DAZKGS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2030
                     /locus_tag="ACHOQY_004714"
     CDS             <1..2030
                     /locus_tag="ACHOQY_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9680647.1"
                     /translation="SNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHIN
                     TSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIA
                     RGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYVPYS
                     SVPVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQ
                     AFNLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGY
                     RYSTQGFYNLSDSAYSRMSGYTVKPPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQL
                     GNYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLA
                     FTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQV
                     GNTHGGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFG
                     QPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNV
                     ELDETVITVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVA
                     ENGQVYLTGLPQSGKLQVSWGKDKNSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            2044..2574
                     /locus_tag="ACHOQY_004715"
     CDS             2044..2574
                     /locus_tag="ACHOQY_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9680648.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2587..3090
                     /locus_tag="ACHOQY_004716"
     CDS             2587..3090
                     /locus_tag="ACHOQY_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9680649.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3149..4063
                     /locus_tag="ACHOQY_004717"
     CDS             3149..4063
                     /locus_tag="ACHOQY_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9680650.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1197 a          891 c          956 g         1125 t
ORIGIN      
        1 aatccaacta tgcatatttg aatttacaga gtggcatcaa cattggtagt tggcgactgc
       61 gcgataactc aacgtggagt tataacagtg ggagtagcaa ttcatctgac agcaataaat
      121 ggcagcatat caatacgtcg gctgaacgtg acattattcc cttacgctca cggttaacgg
      181 taggtgatag ttataccgat ggcgatattt ttgatagtgt gaactttcgt ggcctcaaaa
      241 taaattcaac agaagcgatg ttgcccgata gccaacatgg tttcgctccg gtgattcatg
      301 gtattgcccg tggaaccgca caagtgagtg taaaacagaa cggatacgat gtttatcaga
      361 ctactgttcc acccggccct tttactattg atgatatcaa ctctgcggcc aatggtggtg
      421 atctgcaagt aaccataaaa gaggcagacg gcagtattca gacattatat gttccttatt
      481 cgtctgttcc ggttctccaa cgtgctggat atacgcgtta tgcgcttgcc atgggggaat
      541 atcgtagtgg aaataacctg caaagctctc ccaagttcat acaaggtagc ttgatgcatg
      601 gtctggaagg aaactggaca ccttatggcg gaatgcaaat tgcagaagat tatcaggcct
      661 tcaaccttgg tattgggaaa gatttaggac tttttggtgc cttttctttc gatatcacgc
      721 aggccaatac gacacttgca gatggcaccc gtcacagtgg gcaatcggtt aaatccgtct
      781 acagcaaatc cttctaccag acaggaacca atatccaggt cgcaggatat cgctattcta
      841 cgcaaggttt ttataactta tccgacagtg cctacagtcg aatgagtggt tacaccgtca
      901 agcctcctac cggagacacc aatgagcaga cacaatttat tgattatttt aatctgttct
      961 acagtaagcg tggtcaggaa caaataagca tctctcagca gcttggaaat tacggtacga
     1021 catttttcag tgccagtcgc caaagttact ggaacacgtc acgcagcgac cagcaaatat
     1081 catttggatt aaatgtgccg tttggtgata ttacgacttc gctgaattac agctattcca
     1141 ataatatatg gcaaaacgat cgggatcatt tactcgcttt tacgcttaat gttcccttca
     1201 gtcattggat gcgtacagac agtcagtcgg catttcgtaa ttcaaacgcc agttacagta
     1261 tgtcaaacga tttgaaaggc ggcatgacca atctatcggg ggtttatggc actctgctgc
     1321 cggataataa cctgaattat agcgttcagg tcggtaacac ccacggaggt aatacatcgt
     1381 ctggcaccag tggttacagt tctcttaatt atcgtggagc ttatggtaat actaatgtcg
     1441 gttacagtcg gagtggtgac agcagccaga tttattacgg aatgagtggt gggattattg
     1501 ctcatgctga tggcatcacc tttggacagc cgctgggcga cacaatggtt ctggttaagg
     1561 ctcctggtgc tgataatgtc aaaatagaga accagaccgg aattcatacc gactggcgtg
     1621 gctatgccat attaccattt gcgacagaat atagagaaaa ccgtgttgct cttaacgcga
     1681 attcccttgc agataatgtt gaactggatg aaaccgtgat cactgtcatc ccaactcacg
     1741 gtgctattgc cagagcaaca tttaatgcac aaatcggcgg gaaagtatta atgacgttga
     1801 agtacggtaa taagagtgtt ccattcggtg caattgtcac acacggagag aataaaaatg
     1861 gcagcattgt cgcagaaaac ggtcaggttt atctgactgg acttccacag tcagggaaat
     1921 tacaggtttc atggggcaaa gataaaaact caaactgtat tgtcgagtac aagcttcctg
     1981 aagtttctcc tggtacctta ctgaaccagc agacagcaat ctgtcgctaa ggaacaatag
     2041 attatgaaat acaataacat tattttcctc ggtttatgtc tggggttaac cacctattct
     2101 gctttatccg cagatagcgt tattaaaatt agcgggcgcg tcctcgatta tggctgcaca
     2161 gtctcatcgg attcgcttaa ttttaccgta gatctccaaa aaaacagtgc cagacaattt
     2221 ccaacgaccg gtagcacaag tccagccgtc ccttttcaga ttacgttaag tgaatgcagc
     2281 aaagggacaa cgggggttcg ggttgcattt aacggtattg aggatgcaga aaataatact
     2341 ttgttgaaac tggatgaagg aagcaatacg gcctccggtt tgggtataga aatattggac
     2401 gcaaatatgc gtccggtgaa actgaatgac cttcatgccg ggatgcagtg gatcccactg
     2461 gtaccagaac agaacaatat tttgccttac tccgctcgtc tgaagtcaac tcagaagtcc
     2521 gtcaatccgg gactggtgag ggcttcggca acctttaccc ttgaatttca ataatcagag
     2581 tcagatatga aaagacttca caagaggttc ctgttagcta cgttttgcgc gttattcaca
     2641 gcaactctcc aggccgccga tgtcactatc actgttaatg gtcgggtagt cgctaaaccc
     2701 tgcactattc aaaccaaaga agctaacgtt aatctcgggg atctttatac gcgcaatctg
     2761 caacaacctg gttctgcatc tggctggcac aatattactt tgtcattaac cgattgtccg
     2821 gttgaaacaa gtgcagtgac ggcaatcgtg acaggttcaa ctgacaatac gggttattac
     2881 aaaaatgaag gtactgccga aaatattcag atagagctga gggatgacca ggatgctgcg
     2941 ttaaaaaatg gcgatagcaa aacggttatt gttgatgaga tcactcgtaa tgcacagttt
     3001 ccacttaagg caagagctat cacggtgaat ggaaacgcaa gccagggaac gatcgaggcg
     3061 ctaatcaatg tgatctacac ctggcaataa cgtcgattcg attttcttag ccttcttttt
     3121 aaatcaattt ttaaccttag gatatatcat gggtaaaaca atcagtataa aggtcctgtt
     3181 cggtatttat cttctattaa tggcaggcaa agtttttgcc ttcagttgta atgtcgatgg
     3241 cgggtcaagt attggagcag gtacaacctc tgtttatgtt aatctcgacc ctgtaataca
     3301 gccgggccag aatctggttg tagacttgtc tcagcatata agttgctgga atgattacgg
     3361 cggctggtac gacactgatc atataaacct ggtacaaggc tcagctttcg caggctccct
     3421 acagtcatat aaaggatcgc tgtactggaa taatgtgacc taccccttcc cgttgacgac
     3481 aaataccaac gtattggata ttggtgataa gacaccgatg ccattaccgt taaaactcta
     3541 tattacccca gttggggcgg ccggtggagt tgtcataaaa gcgggggaag tcattgcccg
     3601 tatacatatg tataaaattg caacattagg aagcggaaat ccgcgtaatt ttacgtggaa
     3661 tattatttca aataacaatg ttgttatgcc aacaggcggc tgcaccgttg atagtcgtaa
     3721 tgtcaccgtg gatctcccgg actttcctgg aagcgcagaa ataccccttg gcgtatattg
     3781 ctcaagcgaa caaaagctct cattttatct ttcgggtgcc accactgaca gtagccgaca
     3841 agtgtttgct aacacagcac ctgatgcaac aaaagcatcg ggagtgggtg tcactctaat
     3901 gcgtaatgga aaaatactgg ctacgggaga aaatgtctcc ctggggacag taaataaaag
     3961 caaagtacct ctgggtttaa gcgcaaccta tggacaaaca ggaaacaagg tttcagccgg
     4021 aaccgtccag tcagtcattg gtgtcacttt tatctatgaa taattgtgat tttgacctta
     4081 gtctatgcga tccattcacg aaaattgcgc ggataattat tttgtgaagg ctattagcct
     4141 acacctgtaa gaaaatccgc gcacaattg
//
LOCUS       DAZKGS010000260         4109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKGS010000260 DAZKGS010000000
VERSION     DAZKGS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..500
                     /locus_tag="ACHOQY_004718"
     CDS             <1..500
                     /locus_tag="ACHOQY_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9680651.1"
                     /translation="EFIGQDGAKTCEELAGKFGVSTRKVASTLAVVTATGRLARVNQN
                     GKFRYCMSGGNLPADPKAAPVTKNDGKAFPQPAGAALPVREAATQEEIKTESVAVTVQ
                     SQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALRELNKCRDILRDITAT
                     REQQR"
     gene            533..814
                     /locus_tag="ACHOQY_004719"
     CDS             533..814
                     /locus_tag="ACHOQY_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9680652.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            811..1038
                     /locus_tag="ACHOQY_004720"
     CDS             811..1038
                     /locus_tag="ACHOQY_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680653.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1031..1342
                     /locus_tag="ACHOQY_004721"
     CDS             1031..1342
                     /locus_tag="ACHOQY_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680654.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            1470..1688
                     /locus_tag="ACHOQY_004722"
     CDS             1470..1688
                     /locus_tag="ACHOQY_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9680655.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            1690..2247
                     /locus_tag="ACHOQY_004723"
     CDS             1690..2247
                     /locus_tag="ACHOQY_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9680656.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            2538..2693
                     /locus_tag="ACHOQY_004724"
     CDS             2538..2693
                     /locus_tag="ACHOQY_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9680657.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            2813..3157
                     /locus_tag="ACHOQY_004725"
     CDS             2813..3157
                     /locus_tag="ACHOQY_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP9680658.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            3279..3551
                     /locus_tag="ACHOQY_004726"
     CDS             3279..3551
                     /locus_tag="ACHOQY_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680659.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            3553..>4109
                     /locus_tag="ACHOQY_004727"
     CDS             3553..>4109
                     /locus_tag="ACHOQY_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9680660.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT         1083 a          890 c         1170 g          966 t
ORIGIN      
        1 ttgagtttat cggtcaggat ggtgctaaaa cgtgtgagga actggcgggt aagttcggcg
       61 tcagtactcg caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac
      121 gcgttaatca gaacggtaaa tttcgttact gcatgtcggg gggtaattta ccagcagatc
      181 cgaaagccgc gccggtaacg aaaaatgatg gtaaggcctt tcctcagcca gcaggtgctg
      241 cgttaccagt ccgggaagcc gcaacacagg aagaaataaa aacggaaagt gtggcggtca
      301 cagtgcagtc acagccgtcg ttcaccagaa agcatccgga tggtctgatt ttaccatcgc
      361 tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag
      421 tctgcgccgc gctgcgggag ctgaacaagt gccgggatat tctccgggat attaccgcca
      481 ccagagaaca gcagcggtga gtgggtggaa gacgtggtgc cgggcggaaa tcatgatact
      541 ccggcagtgt gcgggaacga tgaaggtaaa aagcgttggc gcacttatcg gacgaactga
      601 agcggcagtg agaacgaagg cacgggagct gggcatcagc atgatgttac gtggtgattt
      661 tcacccgtcg gcaaaatatt ctcagcgtga tattgagctg gcgcggcaac tgcatcagag
      721 aggcatgcaa agaagggaaa ttgccagaaa attaggcatg ccgctgcgca tagtgaataa
      781 ctacgtttat ttcgacagga gggtgtctgc gtgaaaatcc tgtatcagga ttacggcccg
      841 gtggggcagg tggttatcag cagtactgta atggagtttc ggaagcataa ccgtgtggtg
      901 gatgctgtgc tgttaacctg tccggggata tcggcgagtc gtgcaggtgt gtttattatg
      961 aagacgaaat tatatggcag taaggcgtgg ataaagaagg cgtatcgtgt agcgttgcag
     1021 gaggttaaca gtgagtgaaa ttaaagaaat gccggtagtt cgtgacggat atggctactg
     1081 gacacatcct gaatatgaaa aattctgtga tggtcgggaa tatatttcaa cggaagagtt
     1141 taacgcctgg atggaggaaa ataatcttca atacgtcctc tgcttcagag atgaaggatg
     1201 tgctgacctt gatgcgtgtg atgctgatat ttctgcatgg gaaccggaac gaccagaggg
     1261 cgatggctgg tttattggtt ccattcatga tacggaagat ggcccggttt gtgtctggtt
     1321 gcgaaataag gctgaagcat aaaggcgata aaccaactaa caactaaata ctgaagattt
     1381 aaatcagaaa cgatttttat taaatcctta accggaggga ttcctgcacc ctcagaacat
     1441 caggaggccg cccgaaaggg cggtaagaaa tgaaacatta tttagaaaaa aattacccac
     1501 gaaagagcag aacaacagag tttctgtttt tcattctgtt tatagtgttg atgataccga
     1561 tatccccgct attactggtc tggataattg gaaggacatt tgaaccagtt attgagctat
     1621 ataccgatgt gacatgggaa tcattcagcg cactgcacaa taaaattaat ccgtataagg
     1681 aaaactgata tgagcactat taccagagaa cgcgcggaga ttaaatcata catcacaggc
     1741 ttcctgagcg actcggcgca cgataacaag tcttcagaca gcctgctggc taatgtgttt
     1801 cgtatcgcgc tggcatcact ggaagcagag ccgatagcaa tggtagtgcc tgatgaaatg
     1861 gatttgctta cctgccatct cgacggtgta actaaaacat atgctgatgg ctggaacgcc
     1921 tgccgcgtcg ccatgcttca ggccggaaac tttcgggaaa ataagaattc gtcaaccaac
     1981 aattttcggg aaatctcgga aacgtcaacc agatctccga taactctgga tggctggata
     2041 agctgtactg agcgaatgcc tgaaaagagc cagaacgtgc ttatttcgat gaatatcgat
     2101 agcgaggctg ggccattaat atattccgca cgctatctcg gaggcacgtt ccggcgcgga
     2161 ggtatagcag ttagtccggg taatgatctt aggcaagcaa cccactggat gtcgctacca
     2221 gaaccgccgc aggaggtgaa tcaatgacct ggcctgaagc attcacaacg gtaggaattg
     2281 cgatggcggt ggcgctggtg gtgtattcga tttgccgctg gggataaaaa cggtttgcgg
     2341 gaaaaggata gttaagtaga attgcagcgg gtgcttgagg ctatctgcct cgggcatgaa
     2401 caccaacggc agatagagaa aagccccagt taacattacg cgtcctgcaa gacgcttaac
     2461 attaatctga ggctcaatcc atgctgaaca catgtaggtt agcctcttac gtgccgaaag
     2521 gcaaggagaa gcaggctatg aagcagcaaa aggcgatgtt aatcgccctg atcgtcatct
     2581 gtttaaccgt catagtgacg gcactggtaa cgaggaaaga cctctgcgag gtgcgaatcc
     2641 gaaccggtca gacggaggtc gctgtcttcg tagactacga atctgagaag taagagacca
     2701 ggcgggggag taatctcccg ccacctctga tgtgtcaggc atcctcaacg cacccgcgct
     2761 ttaccatact gaaaatgctg tttgaatgtt catctctgaa agaggactat gaatgaaaaa
     2821 ggtattgatt gcagcactta tttccggtgt gtcttttggc gcttttgcac agcagggtgg
     2881 tttccagggg ccagaagcag agcgttcaac agtagcgcag gcaaaagaac tgaaggatga
     2941 tgcatgggtt atccttgaag ggagcatcgt taaaaaagtg ggtgatgaac gttatgagtt
     3001 tcgtgacaat agcgggacaa ttgtcacgga tattgatgac agcgtatggg ccgggcagaa
     3061 tgtttctccg aaagacaaag taagaattga gggtgaaatt gataaagacc tgagcagtgt
     3121 tgaagttgat gtaaaggcac tgaaattatt aaagtaaccg cccctgcttg ttaagcccgt
     3181 cttactgacg ggttttctgt ttgtacattc cggcgtattg ccttacaatt cgcgcagtca
     3241 gcctgaacaa ctgacacctg ctgtcaccgg agaatccgat gacacaacac ataaaatccc
     3301 acaattctga agccgacccg gaaattaagc aggggaggcg ttttcgtgcg cctcagtatg
     3361 gctggtttca ctatctgttc tgtacgatcg atgaggcaga tatgcttcaa gaggcgtatc
     3421 tgcgtcgcgg tgtccgtgtg gagcggagtc tgaacgctga tcgtctgacc tggaccgttt
     3481 ctgtatatct tcctgttcgt gcacatctgc cacggacaca tgcctgctac cgtcagcgcg
     3541 tctggaggta atgtgcgggt attacttcga cctgttctgg ttccggaact cgggctggtg
     3601 gtccttaagc ccggtcgtga atcattgcca gtttttcatc gcggcagggt gctggtggag
     3661 ccggaaccga aaaacatgcg ggcgctgcca tctggagcgg ttcctgctgt tcgccagccg
     3721 ctggcggaag ataaatcact gctgccattt ttcagcgatg agcgggtgat tcgtgcagct
     3781 ggcggcgctg gtgcactgtc tgactggtta ttacgtcacg tgaaatcctg ccagtggcca
     3841 cacggcgatt atcatcacag cgaaaccgtt attcacagtt acggtgctgg cgcaatggtg
     3901 ttgtgctggc actgcgacaa ccagctgcgc gaccagacct ccgaatcact tgagcaactt
     3961 actcaacaaa atctgacagc ctggatgatt gacgtcatac gccatgtaat gaatggcacg
     4021 caggagcggg aattatcgct ggctgaatta tcctggtggg cagtctgcaa tcaggtggtg
     4081 gacgcattac ctgaggcagt atcgcgtcg
//
LOCUS       DAZKGS010000261         4095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKGS010000261 DAZKGS010000000
VERSION     DAZKGS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..1695
                     /gene="dpiB"
                     /locus_tag="ACHOQY_004728"
     CDS             37..1695
                     /gene="dpiB"
                     /locus_tag="ACHOQY_004728"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9680661.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1664..2344
                     /gene="dpiA"
                     /locus_tag="ACHOQY_004729"
                     /gene_synonym="citB"
     CDS             1664..2344
                     /gene="dpiA"
                     /locus_tag="ACHOQY_004729"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9680662.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2385..3770)
                     /gene="dcuC"
                     /locus_tag="ACHOQY_004730"
     CDS             complement(2385..3770)
                     /gene="dcuC"
                     /locus_tag="ACHOQY_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9680663.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(3909..>4095)
                     /locus_tag="ACHOQY_004731"
     CDS             complement(3909..>4095)
                     /locus_tag="ACHOQY_004731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680664.1"
                     /translation="NHLNEVYNINRQPMLMLDYFLSAQPQLTPLQLTNLHQSTINLHT
                     LLCDNCQPLNMRFVIYV"
BASE COUNT         1126 a          954 c         1056 g          959 t
ORIGIN      
        1 cgtcaaagag gctcgggaca acccgcaagg aaaacaatgt tgcagcttaa cgagaataaa
       61 cagtttgcat ttttccaaag actggcattt ccgctgcgta tctttttgct gattctggtg
      121 ttctcaatat ttgtcattgc agccctggcg caatatttta cggccagttt tgaggactat
      181 ttaacgcttc atgtacgcga catggcaatg aatcaggcga aaattattgc ctccaatgac
      241 agtgtcatct ctgcggtgaa aacgcgtgac tacaaacggc tggcgaccat cgctaacaaa
      301 ttacaaagag ataccgattt tgattatgtg gtgattgggg accggcactc gatccgcctt
      361 taccatccta atccggagaa aattggttat cctatgcagt tcaccaaaca gggcgcgctg
      421 gagaaagggg agagctactt cattaccggg aaagggtcaa tggggatggc gatgcgcgcc
      481 aaaacgccaa tctttgatga cgatggaaaa gtcatcggcg tggtgtcgat tggctacctg
      541 gtgagtaaaa tcgatagctg gcgggctgag tttttattac cgatggcagg cgtgtttgtc
      601 gtgctgttag ggattctgat gttgctgtcg tggttcctgg cagcgcatat ccgtcggcaa
      661 atgatgggca tggagccaaa gcaaatcgca cgcgtggtcc gtcagcaaga ggcgctgttt
      721 agttcggttt atgaagggct gattgcggtg gatccgcatg gttacattac cgccatcaat
      781 cgtaacgcaa gaaagatgct ggggctgagc tctcccggac ggcaatggtt gggtaaaccc
      841 attgctgaag tggtcaggcc cgccgatttc tttaccgaac agattgatga aaaacgtcag
      901 gatgtggtgg ctaactttaa cggtctgagc gttattgcca atcgggaagc tattcgttca
      961 ggtgatgatt tgctgggggc cattatcagc tttcgtagta aagacgaaat ttccaccctc
     1021 aatgcgcaac tgacgcaaat aaaacaatac gtcgagagcc ttcgtacatt gcgacacgag
     1081 catctcaatt ggatgtcgac gctcaatggt ctgttgcaga tgaaagagta tgatcgcgtg
     1141 ctggcgatgg tgcaggggga gtctcaggcc cagcaacagc ttattgacag cctgcgtgag
     1201 gcgtttgccg atcgccaggt ggcggggctg ctttttggta aagtgcagcg cgcccgggaa
     1261 ctggggctaa aaatgatcat tgtccccggt agccagcttt cgcaactgcc gccaggactg
     1321 gatagcaccg agtttgcagc cattgtgggc aatttacttg ataacgcctt cgaagccagc
     1381 ctgcgtagcg atgaaggaaa caagatcgtt gaattattcc tcagcgatga aggcgatgat
     1441 gtggtgattg aagtcgccga tcagggctgc ggcgttccag agtctctacg agacaaaata
     1501 tttgagcagg gggtcagtac gcgtgctgac gagcccggtg aacatggcat tgggttgtac
     1561 ttgattgcca gctacgtaac gcgctgcggt ggtgttatca ctctcgaaga taatgatccc
     1621 tgcggtacct tgttttcaat ctatattccg aaagtgaaac ctaatgacag ctccattaac
     1681 cctattgatc gttgaggacg aaacgccgct ggcagagatg catgcggaat atattcgtca
     1741 cattcccgga ttcagtcaga tattactggc gggaaacctg gcgcaggccc gaatgatgat
     1801 cgagcgtttt aagccggggc taatcttgct cgataactat cttcctgacg gcagagggat
     1861 taatttattg catgaactgg tgcaggcgca ttatcccggc gacgtggtgt ttaccactgc
     1921 agccagcgat atggaaacgg tgtctgaagc agtacgttgt ggtgtatttg attatctcat
     1981 taagcccatt gcctatgaac ggctggggca aacgctaacc cgtttccgcc agcgtaaaca
     2041 tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt ttaatgccta
     2101 tgctcgcggt gaacctaaag acgaactgcc gaccggcatt gatcccttga cgctaaacgc
     2161 ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg tggcgcaggc
     2221 actgaccatc agtcgcacca ctgccaggcg ttatcttgaa tattgcgcca gccgccatct
     2281 gattattgct gaaattgttc acggcaaagt tggcagaccg caacgcatat accacagtgg
     2341 gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt gcctgtgacc
     2401 gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac caaaccaaca
     2461 agcaccggta ccgaggtgcg ttttacgact tcaaacggcg agatcttcgc catcccggca
     2521 accgcaacga ctacgccaga aacgggcgaa agggtacggc ccaggtttga cgcctgcagc
     2581 atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt cgggatcatc
     2641 tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc cagcatggtc
     2701 agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt agcgatagaa
     2761 atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc agcggcaacc
     2821 agcagcatca ccacgttagc aaacgcatct gccattccgc gataagccac ttccagacca
     2881 gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc aatcagcata
     2941 caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc aaaaatcagt
     3001 acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg caggagcagt ggtggtgatt
     3061 tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata acgttgccag
     3121 aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag cgtcgttttg
     3181 aaggcgaagt caatcagcga catttcggaa gcttgcgccg ccagcaccac atcccctgaa
     3241 gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagcagctgc gccacgactg
     3301 ataccaacgt ttaccatcac cggaaatagg gttgccatca gcaaaacacc cagaccggtt
     3361 gcggaagaga cggccagaga catcagacag gcgacaaaat aggcggcaat catcagcagg
     3421 taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat atcattcgcg
     3481 ccgatatggg tcatgtaagc ggcaaatcca cacagcatca taatcatcat gccgaggtcg
     3541 ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc gctgtagcct
     3601 gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat aatcagcaat
     3661 aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat gtagcgagct
     3721 acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat aatttttcct
     3781 gtctccaggc cccaaagtaa ataataaaaa attcctaaag cttaaggaaa aaatatgccc
     3841 aataaattgg cgatgaatgc tgattaaaat caagaaaaac tgccattaag acattgaagt
     3901 tgctgttttt atacatagat aacaaaacgc atatttagcg gttggcaatt atcacataat
     3961 aatgtgtgta aattgatggt ggattgatgc aaatttgtta attgcaatgg tgttagctgc
     4021 ggctgtgcgc tcaaaaaata atctaacatg agcataggtt gacgatttat attatatact
     4081 tcgttaagat gattg
//
LOCUS       DAZKGS010000262         4070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKGS010000262 DAZKGS010000000
VERSION     DAZKGS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..276)
                     /gene="escO"
                     /locus_tag="ACHOQY_004732"
     CDS             complement(<1..276)
                     /gene="escO"
                     /locus_tag="ACHOQY_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFP9680665.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKS"
     gene            complement(336..1676)
                     /gene="escN"
                     /locus_tag="ACHOQY_004733"
     CDS             complement(336..1676)
                     /gene="escN"
                     /locus_tag="ACHOQY_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP9680666.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1660..3687)
                     /gene="escV"
                     /locus_tag="ACHOQY_004734"
     CDS             complement(1660..3687)
                     /gene="escV"
                     /locus_tag="ACHOQY_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP9680667.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            complement(3684..4037)
                     /gene="cesL"
                     /locus_tag="ACHOQY_004735"
     CDS             complement(3684..4037)
                     /gene="cesL"
                     /locus_tag="ACHOQY_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFP9680668.1"
                     /translation="MNLLIKRNVEEFLRLLGNDFYLFDNRVEIDFNGFSFFIEIIDSN
                     VFITFALEYNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVFSLMNTF
                     QQHVQIIERVRRMTS"
BASE COUNT         1265 a          931 c          729 g         1145 t
ORIGIN      
        1 tgatttatta acatgttgta gatcctcttt tgttcgtttt tgtgaatcga gcaatgaacg
       61 cttttcttta gaaagtgagg attttttctc atataatcgc tgcttttgct catcaaatgc
      121 attgttttta atctggtatt tatacaacga cactttatcc agattcacca gcgatgcaca
      181 atacgcctgc ttagaggcaa tcgactcttt aatggcttgc tcgcagtcat cgagtttacc
      241 atccacctct tttatctgag agttaatctt agccattgat tctctcaatc gattcgctct
      301 gcttttacga atagataaaa ttctgtccaa catattcagg caaccacttt gaataggctt
      361 tcaatcgttt tttcgtaact actgatatct ttggtgcttt gttgtataaa gctctgaatt
      421 gctttcctgt tttttattgc cttatccgct tcaggatctt gccccatcgt gtactcacca
      481 atacgaataa gcagttcaat attcttatag gttgcaataa gctttttgca ttcagccgct
      541 gcacgtaaat gttccgattt aacaacgtta tgcataacac gactggcgct taaaccaata
      601 tcaattgcag ggaaatggtt ttcctctgca agttctcggg taagcacaat atgtccatca
      661 agaatagagc gaacttcatc accgatggga tcattcacat tatcgctttc taacagcacc
      721 gtataaatcg cagttattga accttttggc gcaggtcccg cacgctctaa taatttgggt
      781 aacgaggaaa aaacactcgg agggaatccc cctcttacat caggctctcc cgatgcaagc
      841 ccaacatcac gcgcggctcg ggcataacga gttacggaat ccatcattaa taatacattt
      901 ttgccctgat cccggaagaa ctctgcaatg gttgtcgccg taaatgcggc cttcattctt
      961 tccagcgcgg gacggtctga cgttgtgacg accagtacac atttagaaag cgtggattga
     1021 ggtaaaagcg cgaggaattc atttacttcg cgaccacgct cgccgataag ggcaaggaca
     1081 ataatatccg cggatgcgcc attacaaatc attcccaaaa gcgtactttt accaacgccg
     1141 gaccccgcga aaataccgat gcgttgccca ataccgcaag taagtaaacc atctattgcc
     1201 ctgacgccaa gcgtaaatgg ctgatcaata acctgcctta ataaaggatc ggggggttca
     1261 gcatacaaac tgcgttcaaa cggaagataa ggtgcagtaa tattgctccc cattggtctg
     1321 cctataccat caaccagtcg cccaaggagt tcgtccccaa cacgaatctt gaactcttcc
     1381 ccctgataac ttagccactg gccacaatac atgccggaaa tatgctcaaa aggcagcagg
     1441 aaaacctcat cttcatcaat agcgatcacc tcagctaaac gttgactggg ttctatcttg
     1501 taaaaggcac caatacgcgc ttttggtaaa cgcgccttga taatcgtccc gccgatattg
     1561 gtaatcttgc cttcatatct ggtagacgaa tttaatgata atgtcggcac agtgctattg
     1621 agcactttct ggatacgtgg gtatctttcc aatacagaat catgctctaa aatcatttac
     1681 cgttcctaat actttaagtt cgatattatt cccaacttcc tgaaaagaga gcacggggac
     1741 tgacgggaac gaaccttcaa tcattttctt cacatagcgc ctgatatcta aagcggtcag
     1801 aatgacgcca ttccccgttg gattcacaat attttttaag aagccaataa tctgctcact
     1861 tcgctccggt gagatattca aatacgaacc tgatgacgtt tgtctgatcg actctcgtaa
     1921 ctcattttca atatcagagc caataagcca gacattcaga agtgtaccgc gaacgctata
     1981 gcgacctaaa atatgccgac ggagggcgat acgaacgtat tcgcacaaga taaccacatc
     2041 tttttctttt gtggaccaaa aaataagcgt ttcaaaaata gttcgtagat ctctaattga
     2101 gatattttcc tctacgagac gctgaagaat atccacaatc ttgctcagac caagctgacg
     2161 ctgaagctct ttcacaagtt cgttatattt cctctccatg atgtccatca gataacgcgt
     2221 ttcttgtacg ccgatgaact ctttggcatt agacaataca agtttcttta gcaaaaacgt
     2281 tatcttgtca tttgtttttg cataaacatc catacctgag acgtgagcta gttgttggtt
     2341 agcctcattc gttttataca cgatagagtt agccgaaagc ttttcctgga caaattcaaa
     2401 tgatatctct gcatgcccag cctcaaaata aacagtatca tctgtaagag tatctttgta
     2461 tattgactct tgatagagca aaaattctat agcgctatca ttatttttaa ctggattaac
     2521 aataatctta ggcaatggaa ttccactttc ctcaaagaaa ttccaacgca taacctcaat
     2581 ctgctgtgta atatctgagc tataaatatt actacttaag tttaaaatca acggctcggc
     2641 acccggtgag atattttgac tattagacaa atcagctccc atagcgtcct tattatttcc
     2701 ggttccaaac gttcttttct tttgtaattt ccaggcgata cccaacaggc aaaccgccag
     2761 aaacaagaag accaatgtag gaaagccagg aataatcgcc attacgatca tgagtgcggc
     2821 actgattaaa aaagggcgac tattaacaga aacctgttga acaatatcac ctgcaaggtt
     2881 ttcttctttt tcgctttcac cgggaacacg agtaacaaca acccccgcag tgacggaaat
     2941 aataagcgca gggatctggg caaccaaagc atcaccgaca gacaatacag aaaacaggct
     3001 aagtgcttca ctaaatggca tgtcaaattg ccacattcca atgagcacgc caccaaacag
     3061 gttaactaaa acaatgatga taccggcaat agcgtcccct tttacaaact tcatcgcccc
     3121 gtccattgaa ccataaaact ggctttcctt ttgcactcgg gagcgaagaa cttttgcctc
     3181 aagcggatca ataacgcctg cgcgcatatc accatcaata ctcatttgtt ttcctggcat
     3241 accatcaagg gaaaaacgag cactcacttc agcaacacgc tcagcccctt ttgtaataac
     3301 cataaattgt acaatggtga taatggtaaa aataaccagg ccaacaacaa tattaccgcc
     3361 aacaacaaag ttaccgaagg agtaaataat gtcacccgca tcgtgatgca gcagaataag
     3421 tctggttgta ctaacactga gcgacaggcg catcaacgtc gtaatcaaca ggattgtagg
     3481 aaaagaagtt agctcaagag gattttttat atagattgag agcattaata aaagcaacgc
     3541 tgtcgatata ttaatcgcga taatgatatc caccacaact gttggcaatg gaataatcat
     3601 catcattaca gccataaaga agaagagagc cagaataaga tcgtgatatg actctgcttt
     3661 tttaaatata tttaagagtt tattcatgat gtcatcctgc gaactcgttc aataatctgc
     3721 acatgctgct gaaaagtatt cattagagaa aacacatcaa tattggttaa caaacaactt
     3781 aagcacaatt tgttatcgta gacaaaaata tgttcaatca ctccctgcgt ccgttcaggg
     3841 gcaagggcat tgaaaaaaga aaaaaacgca ttttcgttat actctaaggc aaacgtaata
     3901 aacacattac tatcaattat ttcaataaaa aaagaaaaac cattaaaatc aatttccaca
     3961 cgattatcaa acaaataaaa atcatttcct aataatctta aaaactcttc tacgtttctt
     4021 ttaattaaaa gattcatatg caggctctga agtaagtata tgtgtggata
//
LOCUS       DAZKGS010000263         4060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKGS010000263 DAZKGS010000000
VERSION     DAZKGS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1820
                     /locus_tag="ACHOQY_004736"
     CDS             <1..1820
                     /locus_tag="ACHOQY_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9680669.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHOQY_004737"
     CDS             1817..2023
                     /locus_tag="ACHOQY_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9680670.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..3621
                     /locus_tag="ACHOQY_004738"
     CDS             2020..3621
                     /locus_tag="ACHOQY_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9680671.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3602..>4060
                     /locus_tag="ACHOQY_004739"
     CDS             3602..>4060
                     /locus_tag="ACHOQY_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9680672.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
BASE COUNT          926 a         1053 c         1253 g          828 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ttggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggagaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaatccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     2701 ggcgggcgcg cctcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag tgagctggat
     2881 acgcagtcag cgatggattt tattcttggc gcgaacagta atgagcagcg ggacaagctg
     2941 gcgggctgga ttggtgaaat tgccgcgtat tacgccgccg cgccggtccg gctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggtgac tctctgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cggactgggt
     3121 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     3181 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     3241 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta
     3301 ccttcaaaag cgcgtttcag ctttcaggaa gcccgcagcg cctgggggaa ctgtgactgg
     3361 ataggctccg gtcgtatggc catcgatggt ctgaaagaag tacaggaagc ggtgatgctg
     3421 atagaagccg gactgagtac ctacgagaaa gagtgcgcaa aacgcggtga cgactatcag
     3481 gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagccgg tcttaaaccg
     3541 cccgcctggg cggctgcggc atttgaatcc ggactgcgac aatcaacaga ggaggagaag
     3601 agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct
     3661 gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat
     3721 cagccgcctg acggatgcgg tgtccggcga cagcctgact gcccaggagg cactcgcgac
     3781 gctggcatta tccggtgatg atgacggacc acgacaggcc cgcagttatc aggtcatgaa
     3841 cggcatcgcc gtgctgccgg tgtccggcac gctggtcagc cggacgcggg cgctgcagcc
     3901 gtactcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga
     3961 tccgatggtg gacggcattc tgctcgatat ggacacgccc ggcgggatgg tggcgggggc
     4021 atttgactgc gctgacatca tcgcccgtgt gcgtgacata
//
LOCUS       DAZKGS010000264         4015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKGS010000264 DAZKGS010000000
VERSION     DAZKGS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..695
                     /locus_tag="ACHOQY_004740"
     CDS             <1..695
                     /locus_tag="ACHOQY_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9680673.1"
                     /translation="TFIWAICATIFTFSFGFILALALAKKNIRFAKVWRLIFILPYAI
                     PTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDFLSVPLNAKITVIAVCVWVGAPYFML
                     LIAGALTNISRDLYEASEVDGATKWQQFKEITLPMVLHQVAPTLVMTFAYNFNNFGAI
                     YLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNFQQYHIASVISIIIFIFLSVIAIWQF
                     RRMKSFKDDVGM"
     gene            698..1531
                     /locus_tag="ACHOQY_004741"
     CDS             698..1531
                     /locus_tag="ACHOQY_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9680674.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            1551..3350
                     /locus_tag="ACHOQY_004742"
     CDS             1551..3350
                     /locus_tag="ACHOQY_004742"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9680675.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            3372..>4015
                     /locus_tag="ACHOQY_004743"
     CDS             3372..>4015
                     /locus_tag="ACHOQY_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9680676.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKIL"
BASE COUNT         1153 a          677 c          850 g         1335 t
ORIGIN      
        1 ggacatttat ttgggcaatt tgcgcgacga tttttacctt cagttttggc tttattctgg
       61 ctttagcact ggctaaaaaa aatatacgtt tcgcaaaagt ctggcgattg atatttatac
      121 tgccatatgc catacccaca tttgtaacgt tacttatttt ccgtcttctt ttaaatggaa
      181 ttggtccggt taactcgttt ctgaacagta tcggaatgaa ttcagttgat tttctgtcgg
      241 ttccattaaa tgcaaaaata acagtaattg ccgtttgtgt ttgggtaggg gcaccgtatt
      301 ttatgctatt gatcgcgggt gcattaacaa acatatcccg agatttgtat gaggctagtg
      361 aggtcgatgg cgcaactaaa tggcaacagt ttaaggaaat tacattgcca atggtgttac
      421 atcaggttgc gccaactctg gtaatgacat ttgcatataa tttcaataat tttggtgcaa
      481 tttatcttct gactgaaggt ggtccgacta atccggaata cagattcgct gggcataccg
      541 atatttttat tacctggatt tataaactca cattgaattt ccagcaatat catatagctt
      601 ctgtaatctc catcataata tttatcttct tatcggttat agcaatatgg caattcagac
      661 ggatgaaatc atttaaagat gatgtaggga tgtgacaatg gataaatttt tacgtaaaat
      721 atcaacgctc ttagtttatc tttttttaat ctgcaactcc attcttgtgc ttgggccagt
      781 gatatggacg ataatggcat cttttaaaaa agggaataat ctatttagtt caacatttag
      841 tggcatcgag tttacatttg accactacat aacgttgttc acggatacgc catatatgca
      901 gtggtatttg aacactttca ttcttgctac cgctaacatg ttaatttcac tgttcgttgt
      961 gacgatgacc gcctacgttt tttcaaggta tcgatttcgt ggaaagcgta atactttaat
     1021 gagtattctt gtattgcaaa tgtttcctgc atttttatca atgactgcaa tttatatcct
     1081 gctatcaaag gccaatttga tagacacata tactgggttg cttctggttt atgtaaccgg
     1141 ttcattgccc ttcatgacgt ggttggtaaa aggttatttt gatgctattc caacttcttt
     1201 ggatgaggcc gctaaaatag atggagcagg gcatctgact atttttattc agataatatt
     1261 gccattggct aagccgattc tcgttttcgt tggattagtt ttgttcactg gaccatggat
     1321 ggacttcatc ttgccaacgt tggtgcttcg tagtgaggat aaaatgaccc tggcgattgg
     1381 aatttttagt tggattgctt ctaattcagc agagaattat acgctttttg ctgctggggc
     1441 gcttttagtt gcagtaccta tcacattgtt atttgtattc acacagaagc atataactac
     1501 tggacttgtt agcggtgccg ttaaggaata atttttaggg attagtattt atgattacag
     1561 ttagttcgtt aaaacactca gcaaaaagtg aagatagcta tgcctatgat aacgaaactt
     1621 tgcatgtacg gttacgcaca ttaagaggag aagttgataa agttattctg tggataggcg
     1681 atccatataa ttgggctgaa ggtggtcttg acggcggtaa catggccggg acagaagcgt
     1741 tcggctggat tggcggcaac gagatcgaga tggaacaaga agctgtcacc gaatttcatg
     1801 atcactggtt tgcagtgttc aaacctcaaa aacgccgttg ccgttacggt tttatacttt
     1861 ttgggaaaga gggagagaag tttctttttg gtgagaaaag gtgtgttgat atcagttcac
     1921 cggactgcga agaacgagag ttaagccgct taaataactt tttttgcttc ccttatctta
     1981 ataaaattga cgttctaaat accccctcct gggtaaaaaa tacggtatgg taccaaattt
     2041 ttccagaccg tttctgtaac ggcagacctg aaatatcccc agaaggggta gaaccgtggg
     2101 gcagtgcacc gacttcattc aactttatgg gtggggatct gtggggggtt attgataaac
     2161 tcgattatct ggaagatctg ggtattaatg ggatttattt ttgtcctatt tttacctcag
     2221 cttccaacca taaatatgac accatcgatc attttagtgt tgatccacat ttaggtggga
     2281 atattgcttt ccatactttg attgaagaag cgcataagcg tggaataaaa atcatgttgg
     2341 atgcagtatt caatcattta ggcgctgact ctccaatatg gcttgacgtt gtacgcaatg
     2401 gtgccaattc acgttacgcc gattggttct ggattcataa atttcctgtt tatcccgata
     2461 ctccaaaaag tgaatgggat tttaaaaact ttaactatga aacctttggc aatgtaattg
     2521 aaatgcccaa gctaaatact gagaatgaag agtgccgtga atacttactt agtatagttc
     2581 gctattggac gcaaaatttt gatattgatg gttggcgtct ggatgttgcg aatgaagtgg
     2641 atcatcattt ctggcgagat ttcagaaagg tgattaaaga tatcaaacct gaatgttata
     2701 ttttgggtga gatatggcat gagggcaccc cctggttgcg tggtgatcag tttgattcat
     2761 taatgaatta tcccttaaca tatgggatta ttgattattt tgcgttacag gatacaacaa
     2821 aacaagaatt tatgacatcc gttacacgtt cttatctttg ttatcctaaa aatataactg
     2881 aagttatgtt taatcttctg gatagtcatg acaccgcgcg tattttaagc gtttgtttga
     2941 atgataaacg aaaagttaaa ctcgcctatt tatttatgtt aactcaagcg ggctctcctt
     3001 gtatctacta cggtagcgaa ataggaattg atggttttaa atcaatgacg cttgagaata
     3061 atagaaaatg tatgatatgg gatgaaaaca aacaagatct tgaattacgg caatttatac
     3121 gttggttaat acgtctacgt aaaaaacatc cacaatggtg tgtggcatca atacaatgga
     3181 aagatgttga acatcctacg gtgatcgctt atcaacgcga taacatcact ttcttcttaa
     3241 ataatagtga agatactgcc aactttattt atgacggaag aagtatggag atctccggtt
     3301 tcagttatga aattgaaggt ttacctgctg ctgatcttta tgatttttaa atttcaatta
     3361 ggatgtttgt tatgataatt gatgcacaca ctcatattgg tgactttgtg aaaatccgta
     3421 tgccagaaga tgtttttctg gcatctctgg acaagtataa tattgatttt gcattatgtt
     3481 cttgtggatc tgcagtagaa gttgaccatg accagaaccc gattccggat gaagatcaag
     3541 ttacacagca tgataataat gaacgtatgc tcagacttgt tcgtcaacat tcaaagcgaa
     3601 ttggcgcgtt tatgtggatt aagcctcgtc tggaaagttg tgatcaagat tttgaagata
     3661 tgattgccag caatcgagat atcatttatg gcatcaaagt tcacccatat cattcaaaaa
     3721 tggctttcaa ttcagataaa gttcaggaat atatacgcct tgctcaaaaa tatgatctcc
     3781 cggtagttag ccatacttct aatgattatg aatccagccc acagttggtt tatgagatgg
     3841 cacttaaata tccaaatgtg aattttgtta tgtgtcatat ggggctcgcg acagataatc
     3901 aggaagcgat cgaattgatt gcaaaacttc ctaatctcta tggcgatact gcgtgggtta
     3961 gggcggatat ggtttaccag tcgataaaaa tctgtggtag tgataagatt ttatt
//
LOCUS       DAZKGS010000265         3887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKGS010000265 DAZKGS010000000
VERSION     DAZKGS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..577
                     /locus_tag="ACHOQY_004744"
                     /pseudo
     CDS             1..577
                     /locus_tag="ACHOQY_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(588..659)
                     /locus_tag="ACHOQY_004745"
                     /pseudo
     CDS             complement(588..659)
                     /locus_tag="ACHOQY_004745"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            722..868
                     /locus_tag="ACHOQY_004746"
                     /pseudo
     CDS             722..868
                     /locus_tag="ACHOQY_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            895..999
                     /locus_tag="ACHOQY_004747"
                     /pseudo
     CDS             895..999
                     /locus_tag="ACHOQY_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1303..1596
                     /locus_tag="ACHOQY_004748"
     CDS             1303..1596
                     /locus_tag="ACHOQY_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFP9680677.1"
                     /translation="MVKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVI
                     EEAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(1916..2047)
                     /locus_tag="ACHOQY_004749"
                     /pseudo
     CDS             complement(1916..2047)
                     /locus_tag="ACHOQY_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2329..3687
                     /locus_tag="ACHOQY_004750"
     CDS             2329..3687
                     /locus_tag="ACHOQY_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFP9680678.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         1085 a          874 c          946 g          982 t
ORIGIN      
        1 tacagttcac ttacaccgcc tctcagcccg gtaagcacca gaaaatcatt gatatggcca
       61 tgaatggcgt cggatgtcgc gccagtgcac gcattatggg cgttggcctc aacacggttt
      121 tacgtcactt aaaaaaatca ggccgcagtc ggtaacctcg cgcatacaac cgggcagtga
      181 tgtgattgtc tgcgctgaaa tggacgaaca gtggggctac gtcggtgcta aatcacgtca
      241 gcgctggctg ttttacgcgt atgacaggat acggaggacg gttgtggcgc acgtcttcgg
      301 tgaacgcact ctggccacac tggagcgtct tctgagcctg ctgtcggcct ttgaggtcgt
      361 ggtatagatg acggatggct ggccgctgta tgaatcacgc ctgaagggaa agctgcacgt
      421 tatcagcaag cgttacactc agcgcattga gcgacataat ctgaatctga gacaacatct
      481 ggcaaggctg ggacggaagt tactgtcgtt ctcaaaatcg gtggagttgc atgacaaggt
      541 catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta cctcctcccg
      601 aactcaaagc tatcatacag ataattactg gttaacccta tatagggcat tttaaagtgt
      661 tgtgatctta cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt
      721 gattcacaag acaaaacagt ttcaaccagt aaaaaaacgt aaacaccatt cgcatgaatt
      781 tcgtagttaa gatctgaagc ttgcagaatg catcggtgtt actgccggag ttcgtgaact
      841 tagtctgcat gaatcaccac agctttacat ctgtcgcagt aaacagcaaa atcagcaaac
      901 ttacttaatt atatcgagtg cgattacaat cgctggcgat ggtacagttg gtgtggcggt
      961 ctcagtccgg aacaatttga aaatcagaat ctcacttaaa acggcatcca ttttacaaaa
     1021 gatggccgtc tgtgtatttg taatgaatac cttctacatg tttagagtgc ataatccacg
     1081 acaaggcgat atcctaaaaa ttagctagca ggcaactttg atggcataga taatgttttt
     1141 agtaaaaaag agttgactga atataataca gcctcttcct acataagaag gaatgtgctc
     1201 acaaaaaaaa accattagtc tgcttcctgt tcttacaata atcagtatta tgtattttat
     1261 ttatatagca aaatatgctt gttacacctg tcataattag gcatggtaaa attattgact
     1321 gaaccctggg ggagaaagat gcacagtgat tattataata tggtttttgg tgaaaaattg
     1381 gccaatattt tatatgaagc taactctcaa tttttttacg agagaaatgt catagaagag
     1441 gcagtcaacg ctttattctg tgagcgtgaa ataataaaca acaagaacat aataaagaag
     1501 ctgatgttct ttttgtcaga tgttaatcac accaagaaag atgttgttca gagcgcactt
     1561 aatattatca ttgacattac atcaggtgat atttgagatt ttacataaaa gataatctct
     1621 caaggagtct tatctgaaaa cgaaatatat agtccctctc gttttgtaat atcgattggc
     1681 tttgttgaat cggttacgta ccccggagcc ggtcatattt tcaggtaata cacacgcttt
     1741 cagacatttc ggctttcgtc attttggtat catgggcata gcttctgcta cctgaccatc
     1801 ataatcacgc agcttcagtg aagcagtgac cacctgttta acccggtgta tcgttatgat
     1861 cttatccagc aattgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg
     1921 ctgccagatc atcgtttccg atggaagcat aataagcttt ttctgcttct gccggtggaa
     1981 tatggccctg cctttccagc aatcgtcgat tgttatacca gtccacccac gtgagtgtgg
     2041 tcagctcatc atactgacac gtgcattacc cctaccgcga taatgcaacc gtacaatata
     2101 gggcgtttcc taaacgcagc cctcatcact ccaatccaac aagacacagc atttttgatc
     2161 acgaagaaag acaaataatt tccgtaaatg tttccgttgt tacaaataac aacagtgttt
     2221 tatatttctt tggcctcagg cccgcgcatg ttgccattcg atgtcatttt cctgactcgt
     2281 ttcttcttta tggtgtagtg tgatcttcac tttatggatg ctaacaatat gaaaagaaaa
     2341 attattcccg tgctaatcgg ttgtacgctc tctttctctg ccctggcggc acaacctacg
     2401 gctgagcgct atgtcgtcag ttttccagaa ggcacgcacg tgaactatgc cggcgcattt
     2461 gccagtgcat tcccgaacgg ccttccggtt gggataggtt ctggcctgct gttcaccggt
     2521 aaacagggcg atgccctgac attcgcaacc attaccgatc gcggtccgaa cgcggattca
     2581 ccgaaagagg ggaaaaacga aactaaaatt ttcgttacgc cagatttcgc gccgctgctg
     2641 atgacgattc gtgtacaaaa cggcaaagcg gaggccatcg atccccgtcc tctgcatgac
     2701 gataaaggcg cgatcaacgg tctgccgctg gcaagcgacg tgattggctc caccaatgaa
     2761 gtggccttca gcgacacgtt acatagactg aagggggata atcgcgggct ggatacggaa
     2821 gggatcactc cggatggtaa aggaggttac tggctgtgcg atgagtatgg cccgttcctg
     2881 attaacatcg acagtaaagg gaaaatcctg gcaatccacg gtccgcaggc ggcggaaggg
     2941 gaaaaagcga ttgcgggcgg tttgccaaat atcctcaaat ggcgtcaggc gaaccgcggc
     3001 tttgaaggcc tcacccgtat gccggacggg cgtattattg tcgccgtgca aagtacgctg
     3061 gatatcgacg ctaagagcaa gaaaaaggcg ctgtttacgc gtctggtgag cttcgatccg
     3121 gcaagcggaa aaaccgcgat gtacggttat cctatcgaca gcgcggccta cagcaaaaac
     3181 agcgacgcta aaatcggaga catcgtggcg ctcgataacc agcacatcct gctgatcgaa
     3241 cagggaagag ataaaaacaa cagaatgcgt aacctgattt acgaggtcga tctcaataag
     3301 gcgagcgacc tgtcaggctt cgacaagccg ggtgagtatc cggagtttga tgatgagaaa
     3361 accctgtcac agcgcggcat tacccttgcg cagaaaacgc aggttgttga tctgcgctcc
     3421 ttgggctggc agcaggagaa ggctgaaggg ttggcgctga tcgacagcaa aacgctggcg
     3481 gtggcgaacg ataacgactt tggcgtgaag gtggcgatgc aacatccggt cgagggcaag
     3541 acattcaagg attatcgggt gaacgcggaa ggaaagctga cgcttgacga caagcaggtt
     3601 gaaacgaccc tcagagtgaa gccactggag aaaccggagt ctgacagtga actgtggatt
     3661 gtgacgctgc cggaagcgtt gaaataagtt tataaggtac ggacagcagt atcgcttagt
     3721 ttttcaaatt catgtatgtg aacaatattg gggttccggc gtagaccccc aatgaaagcg
     3781 tcaccacgtg aaaacatgcc tgcacggttt acacgtcatc tcatggtggc agccggttaa
     3841 ctttcctgct acaccagtgg aaaaaggcca taagcagtcc gaacaaa
//
LOCUS       DAZKGS010000266         3762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKGS010000266 DAZKGS010000000
VERSION     DAZKGS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..206)
                     /locus_tag="ACHOQY_004751"
     CDS             complement(9..206)
                     /locus_tag="ACHOQY_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9680679.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(448..978)
                     /locus_tag="ACHOQY_004752"
     CDS             complement(448..978)
                     /locus_tag="ACHOQY_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9680680.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(987..1346)
                     /locus_tag="ACHOQY_004753"
     CDS             complement(987..1346)
                     /locus_tag="ACHOQY_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9680681.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(1359..2405)
                     /locus_tag="ACHOQY_004754"
     CDS             complement(1359..2405)
                     /locus_tag="ACHOQY_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9680682.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(2407..2679)
                     /locus_tag="ACHOQY_004755"
     CDS             complement(2407..2679)
                     /locus_tag="ACHOQY_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680683.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            2815..3072
                     /locus_tag="ACHOQY_004756"
     CDS             2815..3072
                     /locus_tag="ACHOQY_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9680684.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            3078..3377
                     /locus_tag="ACHOQY_004757"
     CDS             3078..3377
                     /locus_tag="ACHOQY_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9680685.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(3582..>3762)
                     /locus_tag="ACHOQY_004758"
     CDS             complement(3582..>3762)
                     /locus_tag="ACHOQY_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680686.1"
                     /translation="GKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMS
                     YLERNGLISVEDILR"
BASE COUNT          898 a         1026 c          855 g          983 t
ORIGIN      
        1 aaaagccttc aggactgaag gcgtctgtaa caaccgcact gatagtctgc cagacccgcc
       61 ataacaagct gggtcagtat taactggcag cgttcgcgtg aaaggtaagt attctgcgca
      121 acctccccga ctgtcgccgg ttcggtaacg cttaattcat taaacaccac tctggcggtt
      181 tcggtcatat cctgctgttt cagcatgtct ttttcccttt ttcggttaac gtgacacacc
      241 aataactctt gtcgaaaaag ccagcaagct gaaagaacgg tattaataac caccagcgaa
      301 tttattgcgc tgctgtatat tacggacaca aaaaaaccac cttccggtgg cttccttgtg
      361 cgaaaaaact tgcatttcgc ctcgcgatac agctttgcga agcttacagg aattcaagct
      421 gtttctgcgt aaaaaagcaa gcttttttta tcgaaatgaa tcgtgcatag gtacataaag
      481 catgtgttca gccacggcta accaacctgc aatacgtttc tcacatgtgc tgaaacacca
      541 ttccgggtga gtacgattta aacattctgc catttttctc ttactcattc cccgtccttc
      601 gtacctttgc cggagaatat tgattagccc gggatattcc ccaagcacct cactgataac
      661 gcgatcaata atcaacgcct ctgtgtctgt acaatgtgac aaccagctct tctgcttccc
      721 tctggtcata tcccgaaaaa atgcctcaag ttccggtttt tccagcccgg atttcttcat
      781 gctgcgtaaa acctcattaa ctgctgtttt cgtcagcttt ttcgaaacca gtaaccggtt
      841 aaacatattt ccggatttac ccccaccgat atacgaccac cgcccccaca tccgtaattt
      901 cccctggatc cagactgctt ccagcgtgtt caggcgtaaa tgttcgccgc ttttgcctgt
      961 aatttccgga tatatcatat ttacgctcac tcactctcaa ttttgtaaat cttcacaccc
     1021 agccgtccac cagatactgg ctgaccgcgc acaatattga tttcatcaaa ctgctcgtca
     1081 tcaatgagca cttccgcatg cgtcagcgca tccagcggtg ctttcagaat gttgtccagg
     1141 tcacggcggc gcttatccgg tggctctgca atcaccttta tcgccagcct tccggacagg
     1201 cttaatttca gccgctgctg gcgaacaata agcgccacag cccggcgata acgctttccc
     1261 tcctccgaga taaaatatgt gctgccacgg cgtcgccagt aagtgttcac cgtcggcggg
     1321 taaggtaaaa ccaaatctat gagcatcagt cacctctttt acccaagcac gccagttgca
     1381 aaggcgtgat caagaaaacg aaaaattaaa tcaacctgag aaccatgctt ttcttcgaac
     1441 gccagcggat ccgcatgaag ctcgttgtga tgctcccgac acagcggtag cgtgaaaata
     1501 tcgtgagatt ttgtccccat tcccccctga ccatgaccaa tcaggtgatg cggatcgtca
     1561 gcaggcttac cacaacacgc acacggctgt gtcttcaccc agtgcgtgta tttctcgtta
     1621 acccagcggc gacgtttagg tcgtttcatg aaagattccg gagactcagg atcaacggca
     1681 atgctgacca ccgtcttttc ctgtggcggt ttttgctggt gagcgtgagg cagcggcgca
     1741 agattttttg tgcgctgctt cagtatgctg gtggcggtct gctctcccgg tacgatgtcg
     1801 ctttcacggt acattgagcg gattttttcc gcacgcaacc ccagcgaacg acgtaatacc
     1861 gcttccggta gcgcgtccgc cacctgattg cggaccgccc accaggataa ttcagccaga
     1921 gataattccc gttcctgtgc accacttatt gcgtgacgta tgacgtcaat catccaggct
     1981 gtcagatttt gttgagcaag ctgcccgagt gattcggatg tctggtcgcg cagctggttg
     2041 tcgcagtgcc agcacaacac cattgcgccg gtaccataac ggtgaatgac ggtttcgctg
     2101 tgatgataat cgccgtgtgg ccactggcag gatttaatat ggcgcaacag ccagtcagac
     2161 aatgcaccag caccaccagc agcacgaatc acccgtgcgt tactgaaaaa cggcagcaat
     2221 gttttgtctt ccactagcgg ctggcgaacg gcaggaacaa ccccggacgg cagattacgc
     2281 atgcttttcg gttccggctc caccagtacc cgggtattgt ggaataccgg catggattca
     2341 cgacccggct taacgatcac cagcccgagt tccggtacca gaacaggtcg aagtaatacc
     2401 cgcacgttac ctccagatgc gttgctggaa tgtgcgggac ggacgcggcg ggcgttcgga
     2461 ataaaggagc ctgacgtaga ttatccagtg acggtagtcg aggctaaggg ctttcttaaa
     2521 ctcataccca cgtctgcggt agttatgaat cagccattcg gcctgttctt cagtgcaggg
     2581 atcgtgctga taccagtcat atttgaatgt gtgagaacac cgcccttgcc tgctggcagg
     2641 ggcgatatca gaattgtgat gtttggtatt gtgcgccatc ggttttctct gctggcgcag
     2701 caggtgccag ttgttcaagc tggcgtgcgg caatattgtc tctgatttct gttgtcgtca
     2761 acaggcaacg tgctatcatc gaatggtgtt ctatcctact ccgtgaggtt taccatgcgt
     2821 acaacccaac aattcagcat tacattaact aacgaaatgg ctgacatggt gcgcgcccgt
     2881 gtggcttccg gtgcctatgc ttcagaaagc gaggtcattc gtgaagggct tcgcgcactg
     2941 aatgagcgcg ataaagcaat cgaagcgtgg ttaacgcatt cagccgcccc ctctcttgat
     3001 tctatccgcg aaaatccaaa caacggacgc tccatttcac aggttcgcgc cgcgattcga
     3061 tccgggaagt aatctgcatg acatatgaag tcatcattac tcctgaggcc gaacaacaaa
     3121 taatcaacct gcacaggtat ataacggaga aagcagggaa cgtcattgct gacaattatg
     3181 ccaatgcgct tcttgattat cttgatgggt tttctacatt cccgcatcgg ggcaataaac
     3241 gcgatgatat tcgccagggg atgcgggtaa ctcatttccg ccacagaacg attattgctt
     3301 ttgccgttga tagcagaaaa gtctttattg tcggtatcta tcatggtggg caaagttatg
     3361 aaaccgattt cttataaact tttacccaca tcattccggt gttagaatta accgtccgcc
     3421 ccctctctta ctggcggatt cgtaggctat ataaatcaaa gatcccggct catgtttatg
     3481 tcgggatctt ttttcggcga tttatcccca gcggcaaatc gaatacacca ccagcgccac
     3541 cgccatcgca attcctaccg ttgtgaatgc ttcaggccag gtcatcgtaa aatatcctcc
     3601 acgcttatca gtccgttccg ctccagataa ctcatcgcct tatccggtaa tttgcagtct
     3661 ggcttcgctt tcctcagttg ccaggttaac tgctttacca gcatggttaa ctcatcgacc
     3721 agacgctgat atcccactgg tttgtattca tgcaatttac cg
//
LOCUS       DAZKGS010000267         3758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKGS010000267 DAZKGS010000000
VERSION     DAZKGS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHOQY_004759"
     CDS             <1..75
                     /locus_tag="ACHOQY_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9680687.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHOQY_004760"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHOQY_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9680688.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1540
                     /locus_tag="ACHOQY_004761"
     CDS             863..1540
                     /locus_tag="ACHOQY_004761"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9680689.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLSVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            1540..1722
                     /locus_tag="ACHOQY_004762"
     CDS             1540..1722
                     /locus_tag="ACHOQY_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9680690.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            1695..1886
                     /locus_tag="ACHOQY_004763"
     CDS             1695..1886
                     /locus_tag="ACHOQY_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9680691.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            1963..2178
                     /locus_tag="ACHOQY_004764"
     CDS             1963..2178
                     /locus_tag="ACHOQY_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9680692.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2498
                     /locus_tag="ACHOQY_004765"
     CDS             2277..2498
                     /locus_tag="ACHOQY_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9680693.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2495..2800
                     /locus_tag="ACHOQY_004766"
                     /pseudo
     CDS             2495..2800
                     /locus_tag="ACHOQY_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            3097..3447
                     /locus_tag="ACHOQY_004767"
     CDS             3097..3447
                     /locus_tag="ACHOQY_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9680694.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            3522..>3758
                     /locus_tag="ACHOQY_004768"
     CDS             3522..>3758
                     /locus_tag="ACHOQY_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680695.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQR"
BASE COUNT         1071 a          858 c          990 g          839 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggctggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gctgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcatcg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa cgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gcacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gagtggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacgtcata
      541 aagccatgat tcagtgtgcc cgtctcgcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aataccgcat acactgcaga acgtcagccg gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg aaattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgtg
      781 catcgtcaga actgacacag gccgaagcag taaaagctct tggattcctg aaacaaaaag
      841 ccactgaaca gaaggtggca gcatgacacc ggacattatc ctgcagcgta ctgggatcga
      901 cgtgagagct gtcgaacagg gagatgatgc gtggcacaaa ttacggctca gcgtcatcac
      961 agcttcagaa attcacaacg taatagcaaa accccgatca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taacgctaag gctctggcct ggggaaaaca gtacgagaac gacgccagaa ccctctttga
     1141 gttcacttcc ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgagc tgaaatgccc
     1261 gtttacctcc cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacacga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatatgggga gcaatggtaa tgaagcatcc tcacgataat
     1561 atccgggtag gcacgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatccgaaa tccactgaaa gcacagcggc tggctgaaga gataaataat
     1681 aaacgagggg ctgtatgcac aaagcatctc ctgttgaatt aagaacgagt atcgggatgg
     1741 cacatagcct cgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggcg gcgaaagcag
     1861 aagcaaacga gagagacccg gcatgacaac aacagaatgc atttttctgg cagcaggctt
     1921 catattctgt gtgcttatgc ttgccgacat gggacttgtt caatgacacc tcagcaggaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcag
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac tgaagaagca ccgcgaaacg
     2101 gtaacgctaa tgggattcac accgactcat ttaagtctgg cgatcggcat gttaaacggc
     2161 gtctttaagg agcgatgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcaccctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaattg aagaattaca gcgcaacaca gcaataaaaa
     2341 tgcaccgcct gaaccaccag gctatatctg ccactcattg ttgtgagtgt ggcgatccga
     2401 tagatgaact aagacgtctg gccgttcagg gttgtcggac ttgtgcaagt tgccaagagg
     2461 atctggagct tatcagtaaa cagagaggtt cgaagtgagc gaaattaact ctcaggcact
     2521 gcgtgaagcg gcagagcagg caatgcatga cgactgggga tttgatgcgg accttttcca
     2581 tgagctggta acaccatcga ttgtgctggc actgctggat gaacgggaaa gaaaccagca
     2641 atacatcaaa cgccgcgacc aggagaacga ggaaattgcg ctaacggtag ggaagctgcg
     2701 tgttgagctt gaggaagcaa aatcaaaact caacgagcag cgtgagtatt acgaaggtgt
     2761 tatctcggat gggagtaaac gtattgctga actggagaaa agcgaagagc aactcattaa
     2821 cgagcgtgac catgctgagt ctgctttagc tgatatgtac ttcgcaacaa ccggggatag
     2881 gcctgagtgg agtaactgtt tcagtttttc agatgctgtc gatgccgtag ttgacagaat
     2941 tgctgattta gaagccaaac agccatcgcc agtagtaccg gaagaaaaac caatgcctaa
     3001 ccctcttagc atgtacacag ttgatgctgt tgcagctatt gcagaggtga gaggctggaa
     3061 cgcctgccgt gcagccatgc ttaagggaga taaatcatga ttaatcgaac caaactggag
     3121 cacatcctcg agtatgccag gcagcagagg cgctttggcc agctttgtaa aattctgcca
     3181 ggagatatgg ttgaaatcgt ggagattgcc atgcgtaaga ctggcaactc tccggtaagt
     3241 ccgggtggtt ggataagctg tagtgagcga atgcccgctc aagatgattg gattttaatt
     3301 tattcaaagc acggtgagta tatggcagga caggtacaag aggaatacgt ggagttgagc
     3361 gacggcactt tatcgtggtt agggaacgcc ttgtactgga tgctgctacc agaaccgccg
     3421 cagggagtga atgatgaatt ggcctgaagc attcaccgct gtaggagttg caatcgcggt
     3481 ggcatttatt ctgtattcgc ttttccgctg gggataaagg aatgttcgct ctgattcaac
     3541 gtggtcagat atacaccgat agcgccggct acccgataaa aattcttcgc tgcataaaca
     3601 acactgtgtt gtacagaaga atggatgggc gaacacagtc ggtaaaaata aacgatttta
     3661 atgaactgtt tgaacggatc gatcaccagg aataccgaca aattctggct gaaacagagc
     3721 aggagaacca tctgaaaaaa ttacgcgcca tgcaaagg
//
LOCUS       DAZKGS010000268         3732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKGS010000268 DAZKGS010000000
VERSION     DAZKGS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            146..1249
                     /gene="proB"
                     /locus_tag="ACHOQY_004769"
     CDS             146..1249
                     /gene="proB"
                     /locus_tag="ACHOQY_004769"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9680696.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1261..2514
                     /gene="proA"
                     /locus_tag="ACHOQY_004770"
     CDS             1261..2514
                     /gene="proA"
                     /locus_tag="ACHOQY_004770"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9680697.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2629..2704
                     /locus_tag="ACHOQY_004771"
     tRNA            2629..2704
                     /locus_tag="ACHOQY_004771"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2662..2664,aa:Thr,seq:cgt)
     gene            3309..3467
                     /locus_tag="ACHOQY_004772"
     CDS             3309..3467
                     /locus_tag="ACHOQY_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680698.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          947 a          875 c          996 g          914 t
ORIGIN      
        1 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
       61 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
      121 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
      181 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg ttgaacttgt
      241 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
      301 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
      361 acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
      421 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
      481 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
      541 aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata acgataacct
      601 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
      661 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
      721 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
      781 cggcatgagt accaaattgc aggctgctga cgtggcttgc cgtgcgggta tcgacaccat
      841 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
      901 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
      961 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc ttgaacgcgg
     1021 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     1081 catccgcatt tgcaacctcg aaggtcgcga tatcgcccac ggcgtcagtc gttacaacag
     1141 cgatgcatta cgccgtattg ccgggcacca ctcgcaagaa attgatgcaa tactgggata
     1201 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagcaggctg
     1261 atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc gcaactctcc
     1321 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     1381 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gtgccaatgg ccttggcgaa
     1441 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgatgatgtg
     1501 cgccaggtgt gtaacctcgc cgatccggtg gggcaggtaa tcgatggcag cgtactggac
     1561 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     1621 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     1681 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     1741 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     1801 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     1861 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     1921 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     1981 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     2041 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     2101 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     2161 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     2221 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     2281 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     2341 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     2401 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     2461 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     2521 ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg cccggttagt
     2581 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     2641 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     2701 accattaaga aaatcaataa cttagcttaa cttctctaag aaattgtatc ttctgttatc
     2761 acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat gggcgagtag
     2821 ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta
     2881 aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct
     2941 acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc
     3001 atagtggatt aaagcccacc tcttcaaaac ctaacttgat cgggaaagaa tcaagctcta
     3061 gcacggtttc gtccaccgga gtacaacggg tgcctgataa gagaagaact gctaagactt
     3121 catagctttt gtgaaaaagc cctttttagt ggatacgcct acggcgctta ttttttggta
     3181 taaccatcaa aggcgcttta tggctcctat tgtcttgaaa atgtatcttt acttgtattg
     3241 tgttttatgc gtagtctccc tgtgtagtat gtaacttgtt aattttcata tgaatacaga
     3301 ggcacacgat gaaaaacatt gcagctatca aacgtaataa ccgcaagatt cacgctcgta
     3361 agttcctgtc tacgccagag ggaaaagcct ggctagagcg aaagcaacga gagaacgaag
     3421 aaagaaaact ccttagtgag ttgaaatggc ttagagagga tttctgaaaa tctccagaac
     3481 gcaacaggac gcattctaac gctgttttca ttgcttggca tagggatgta taggtgatgt
     3541 gtgtttaatg cgtatgtgag cttgttttgt gccttatttt gcattttcat aatgcagaag
     3601 agaacacgga aaaccttaaa attaattatt ggggtattta aggctatttt caggttattt
     3661 aagggtatta aacttgattt aattgattag aatataaaca gataattatg tatcttattg
     3721 atttataagg tt
//
LOCUS       DAZKGS010000269         3623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKGS010000269 DAZKGS010000000
VERSION     DAZKGS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..421
                     /locus_tag="ACHOQY_004773"
     CDS             101..421
                     /locus_tag="ACHOQY_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFP9680699.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            436..2769
                     /locus_tag="ACHOQY_004774"
     CDS             436..2769
                     /locus_tag="ACHOQY_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP9680700.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRHLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            3353..3481
                     /gene="rhuM"
                     /locus_tag="ACHOQY_004775"
                     /pseudo
     CDS             3353..3481
                     /gene="rhuM"
                     /locus_tag="ACHOQY_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          841 a         1031 c          991 g          760 t
ORIGIN      
        1 ttcgcgcagg gatacgcacg tcttcaggag acgcagatat gccttatcag ttaatgcaac
       61 cggcacggaa tgcagtcatc tgtcacaggg aggaaagcaa atgaaaacac ccttaccgcc
      121 cgtcttacgc gctgcccttt accgtcgcgc tgtcgcctgt gcctggctga ccgtgtgcga
      181 acgtcagcac cgctacccgc atctcaccct tgagtcactg gaggcggcca tcgccgctga
      241 gctggaaggc ttttatctgc gccagcacgg tgaggaaaaa gggcgtcaga tagcctgtgc
      301 cctgctggaa gatttaatgg aatccggccc cctgaaggcc gcgccgtcgc tgtcctttct
      361 cgggctggtt gtgatggatg aactctgtgc ccgtcacata aaagcgccgg tactgcactg
      421 aaggagaaca acaccatgaa aatgaacgta accgccaccg tcagccatgc gctcggccac
      481 tggccgcgta ttctcccggc gctggggatt caggtgctga agaaccatca tcagccctgt
      541 ccggtctgtg gcgggagtga ccgcttccgt tttgatgaca gggaggggcg cggcacctgg
      601 tactgcaatc agtgtggtgc cggtgacggc ctgaaactgg ttgaaaaggt gtttggtgtt
      661 tccccgtccg acgcggcagc aaaggtggct gccgtgaccg gcagcctgcc cccggctgac
      721 ccggcagtga cggccgccgc cgttgctgaa acagacgctg cccggaagaa cgccgccgca
      781 ctggcacaaa ccctgatggc gaaaacccgt cccggaaccg gtaacgccta cctgacccgc
      841 aagggctttc ccggccggga atgccggatg ctgaccggca cacacagagc cggtggcgtg
      901 agctggcgcg ccggtgacct tgtggtgcca ctgtatgacg acagcggcga actggttaac
      961 cttcagttaa tcagtgctga cggccgtaag cgcaccctga aaggcggaca ggtcaggggc
     1021 acctgtcaca cccttgaagg acagaatcag gccggaaaac gtctgtggat agcggaggga
     1081 tacgcgaccg cacttaccgt gcatcacctg accggtgaaa cggtaatggt ggcgctttct
     1141 tccgtgaacc tcctttctct ggccagcctt gcccggcaga agcatccggc ctgtcagatt
     1201 gtccttgccg cagaccgtga cctcagcggt gacggccaga aaaaagccgc cgcagccgca
     1261 gatgcgtgtg aaggtgttgt tgccctgccg ccggtcttcg gtgactggaa tgatgccttc
     1321 acgcagtacg gcggggaagc cacccgtaag gccatttatg atgccatccg gccaccggct
     1381 gaaagcccgt tcgacaccat gagcgaagcg gagttctccg ccatgagtac cagcgaaaag
     1441 gccatgcgta tctatgagca ttacggcgag gcgctcgcgg ttgatgccaa cggccagctt
     1501 ctgtcccgtt atgaaaatgg tgtctggaag gtgctgccac cacaggactt tgcccgggat
     1561 gtggccgggc tgtttcagcg gctgcgcgcg ccgttctcct ccgggaaggt ggcctccgtg
     1621 gttgacaccc tgaagctgat tattccgcag caggaagccc catcccgcca cctgattggc
     1681 tttcgtaacg gcgtgctcga cacgcagaac ggcacgttcc acccgcacag tccgtcacac
     1741 tggatgcgca ccctgtgcga tgtggatttc accccgccgg tggacggtga aacgctggaa
     1801 acccacgctc ccgcgttctg gcgctggctt gaccgtgccg ccggtggccg tgcggaaaaa
     1861 cgcgacgtga ttctggctgc actgtttatg gtgctggcaa accgctacga ctggcagctc
     1921 tttctggagg tgaccggtcc cggcggcagc ggcaaaagta tcatggccga aatagccacc
     1981 ctgctggccg gggaggataa cgccacgtcg gccaccatcg agacgctgga atccccgcgt
     2041 gaacgtgccg cgttaactgg cttctcactg atacgcctgc cggatcagga aaaatggagc
     2101 ggcgacggtg ccggactcaa ggccatcacc ggcggcgatg cggtgtccgt ggacccgaaa
     2161 taccgggatg cgtactccac gcatattccg gcggtaattc tggccgtgaa caataacccg
     2221 atgcgcttca ccgaccgcag cggcggcgtg tcacgcaggc gggtgattat tcacttcccg
     2281 gaacagatag ccccgcagga gcgcgacccg cagcttaagg acaaaatcac ccgcgagctg
     2341 gcggtcatcg tgcgtcacct gatgcagaag ttcagcgacc cgatgctcgc ccggtcactg
     2401 cttcagtccc agcagaactc agacgaggca ttgaacatca aacgggatgc cgacccgacg
     2461 tttgatttta tcggctatct ggaaaccctg ccgcagacca gcggcatgta tatggggaac
     2521 gccagtatca tcccgcgtaa ttaccgtaaa tacctctatc acgcctatct ggcctacatg
     2581 gaggcaaacg gctaccggaa tgtactcagt ctgaaaatgt tcgggctggg gctgccggtg
     2641 atgctgaagg aatacggact gaattacgag aagcgccata ccaaacaggg gatacagacc
     2701 aacctgacgc tgaaagagga aagctacggc gactggctgc caaaatgtga cgaccctgca
     2761 acaacctgac cctcatgact catctgaccg gcatctgccg gtcttttttc atccctgaat
     2821 cccccgaagg tgaacaatcc actgttcacc cttcaccgta tattcacccg ttatcacact
     2881 gaaattaaaa gagaaaaacg aaaggtgaac agtgtgaaca atcaaatcaa aaaaaacttt
     2941 tttcttcctg agtgatttca gtgcggagga ttaatcaccg gtatgagtca caccggcaga
     3001 atgccggagg tgaagaatcg aatgttcacc cttcacccat tattcaccac ctatcaaact
     3061 gaaataaaag gagaaaacag aaaggtgaac agtgtgaaca gttcttccga aaaaaaattt
     3121 ttttcctgag ttaacgtgcg tgaagatacg tgtaacaatt aatcgataga tattatatgt
     3181 ataaggatgt gtataaatga attagaatag aaaaatataa acgatttgaa tcaagtggtt
     3241 atatggcaat tttgactcct gtgatcttcc gccaaaatgc ctcttctgac gttctccaac
     3301 cccttgtaat actgtcaatc cagatatttt ctactgctca atattcaatc ggcttcagga
     3361 atacctggtc taaggttttg tgatggacga tgagcggctg aagaatccgc ctgtcggttc
     3421 ttctgtcctc gaacgcatcc gcgatatgcg cgccagcatg tgccgtgttt atttgcaggt
     3481 gatttgcccc ctgctggctc tcagccaact gtatcgccat taaggtgaag atgttgtttt
     3541 actcgcccca gaacaccctt ttttatacga ctattctatt tctttaaccg ctggcgaaaa
     3601 aaactgacta taaacttcac caa
//
LOCUS       DAZKGS010000270         3612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKGS010000270 DAZKGS010000000
VERSION     DAZKGS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..363
                     /locus_tag="ACHOQY_004776"
     CDS             <1..363
                     /locus_tag="ACHOQY_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9680701.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHOQY_004777"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHOQY_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9680702.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHOQY_004778"
     CDS             2185..3504
                     /locus_tag="ACHOQY_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9680703.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          871 a          909 c          893 g          939 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctaaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aa
//
LOCUS       DAZKGS010000271         3596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKGS010000271 DAZKGS010000000
VERSION     DAZKGS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1016
                     /locus_tag="ACHOQY_004779"
     CDS             <1..1016
                     /locus_tag="ACHOQY_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9680704.1"
                     /translation="LSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGFNGHVGTTF
                     EYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQEEKGYHENE
                     TSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNSFRPTLTYF
                     NSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIEFFYQKKHN
                     DEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSLQYNANRTY
                     FKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQDNIYVHAF
                     YRF"
     gene            1035..1991
                     /locus_tag="ACHOQY_004780"
     CDS             1035..1991
                     /locus_tag="ACHOQY_004780"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9680705.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            2082..2570
                     /locus_tag="ACHOQY_004781"
     CDS             2082..2570
                     /locus_tag="ACHOQY_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFP9680706.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            2653..>3596
                     /locus_tag="ACHOQY_004782"
     CDS             2653..>3596
                     /locus_tag="ACHOQY_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9680707.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSI"
BASE COUNT         1157 a          652 c          753 g         1034 t
ORIGIN      
        1 tcttatcggc ttcaattgca acagtccttt taggctcaac aggagccttt gcggcaaacg
       61 atcgcggctt tacctccgac actgactggt cagaagaaca agcgggcttt aatggacatg
      121 ttggtactac atttgaatat gagaccaaaa gcactgataa ctatttaggt aaaacagtaa
      181 aggaatatac taaaacacac gagattttcc aggcctattt cagccaacct aattggcaat
      241 ttgcagcaat ctatggcctt aagtctatcg atcgccggca agaagaaaaa ggctatcacg
      301 aaaatgaaac aagtctgcaa aactatatat cactaaacaa aacgttcact attggtaacg
      361 gtttcgactt cagtctggta tacgacctga tgtataccga cggaaatata gatagccctt
      421 atgttacagg cttgcatact gtaactgttg acaacagttt tcgccctacg ttgacgtact
      481 ttaactctga ctggaacgca ggtttttatt caaacgttga atacttatac agtcgaaatg
      541 ataagagctc ctggggcgta cgcgaagaag aaggtcaaag tatactattt cagccctata
      601 aacgttttgg tgcatgggag tttggtattg aatttttcta ccagaaaaag cataacgatg
      661 aatttaatca cggtaaaata aatgatcgta gtatttatac cgaaacttac tacgagccaa
      721 ttatcaaata tgcttttgaa aacgcaggta atctgtatct acgcacccgc ttcggacacg
      781 ggaaaacaga aaacgcagat agtttgcaat acaatgcaaa cagaacctat ttcaaaacaa
      841 ttcgaaaagc cactctaggt tatgaacaaa aaataggtga cgactggata gccaagattg
      901 aatataaaca tgcctgggaa aaagagcaca aaaactttta tgatccaaga gatgaagaaa
      961 aatataatgt gctcaatcag gataatattt atgttcatgc tttctatcgc ttttgattaa
     1021 acagagggaa acctatgttc agcgcactag atattaaaac gttgatgcaa ggaactttat
     1081 atggtgatcc atcactcagg gttgatacca ttcggccaat ccatagtcct ttagttggtg
     1141 gactctcgat tgttatgaca cctggagatt tgttgcatat accaacgact ggtgcggata
     1201 taattatcgg accggaagag atcattagtt ctaatgctaa ggcaaagatt gtagtcgatt
     1261 acctgaacgt gaataatctt aacaaagttt taagatattt taaggtacat aagtatcgtt
     1321 tgtttgagca agaaaacact tcaacgatac ctgatgtata cattggtaaa cactgccaga
     1381 taggtatgaa ctgccatttt atgcctggtg ttaaaattat gaactgtgtg accataggtg
     1441 ataatgttgc cattcatgcg aatacagtga ttaaagaagg tactattatt ggcaatgacg
     1501 ttatcataga ttctaataac tccatcggta attatagttt tgaatatatg tcggatgaac
     1561 gtgattcata tgtccgagtt gatagcatcg gtagagtaat cataggtgat gatgttgaaa
     1621 tcggttgtaa caacaccatt gaccgaggga cacttggaga tacgattata ggccaaggca
     1681 ctcgtattga taatcaagtg cagattggac atgactgcat aatcggtaat aaatgcctga
     1741 ttgtttcaca atgcggtttt tcaggacacg tagttttagg cgaccatgtt attacacatg
     1801 gtcaagtcgg aatcgccggt cacatatcaa ttggtagtta ttcggtaatt aaggctaaat
     1861 caggtgtaag ccattcttgt cctgaaaaga gtgacctttt tggttaccct gccaaaaata
     1921 cgcgagaata caacaaaaat ctcgctgtcc ttaataaact aactaaacag catggtgtat
     1981 ataaacagtg agtcgttgag aagtattatt tatatacatt aacattaaca ctgacataga
     2041 aatagcttaa ctaagctata ctacttttat tgagagttta aatgaaaaat aaattgctta
     2101 ttgcggctgt gttgactggt cttctttcag gatgcgttga tggcaattca ccctctggca
     2161 gtcaggaagg tgtcgtgaaa gcggatcctc gctattcaga atgtaacctc cctgatgtaa
     2221 atgaaagagg gccaattgct gcgggtcttt acatttccgg aaccctcccg gaaggctatt
     2281 ggttaatgaa gccagagcgt aaaatgcatt ataagggaga tggtatttat cagcttattt
     2341 attctgaaaa agcaggtgcg gtaacaatgc aatttgcgac tatgaactgg aacccgcagt
     2401 tcactgtaac aggtcttgaa ttgccattaa atcaggctaa aacactcaag aaaggaggca
     2461 tggataaaaa tacaaaagtc gttattcctg ccgatggaaa gtatgtttgg acgattcaaa
     2521 ttggtcctga taaaaaggca atagccgcca tggtagcacc atgtaaatag ttatttttag
     2581 cactccacta ttcaacattc gttgaatagt ggagtatcag tattccaaaa ttcttgttaa
     2641 tgagagcaga ttatggccgc ggtaattcta cgcaaagttg aaaaaattta tgacaatgga
     2701 ttcaaagctg ttcacggcat tgatctcgat ataaacgatg gtgaattcat ggtttttgtt
     2761 ggaccatctg ggtgtgccaa atcaactaca ttacgcatga ttgcaggtct tgaagacgta
     2821 tcaggtggtg aaatttacat tagcgaccgc cttgttaata cccttccgcc taaagatcgc
     2881 agcattgcaa tggtgttcca gaactatgcg ctgtatcccc ataagactgt atttgacaat
     2941 atggcttttg gtttaaaaat gcaaaaaagg ccaaaagaag agattaagaa aattgtcgaa
     3001 gaagttgcag aaaaacttga aattagcgat ttactttacc gaaaaccgaa agaaatgtcg
     3061 ggtggtcagc gtcagcgtgt cgcggtaggc cgtgcaattg tccgtaagcc agatgttttc
     3121 ttatttgatg agccactatc taaccttgat gcaaaacttc gtgtttcaat gcgagtcaaa
     3181 attgctcagt tgcatcagtc tctcaaggat gagggtcatc cagcaaccat gatttatgtt
     3241 acacacgatc aaattgaagc attgaccctc ggtgatcgta tttgtgtgct taatcagggg
     3301 cgaataatgc aggtcgacac accagttaat ctgtataacc accctgctaa cagattcgtt
     3361 gctggcttta ttggttcccc gtcaatgaat ttgatagatg cggttgtacg tgaggaagac
     3421 ggtaaactct atattgacat tgccaaaaat attcggttgt acatacctga gaataaacaa
     3481 tcagtactca aagcgtatat agataaaccc gtttgttttg gtattcgtcc agaacatatc
     3541 tctttgagtt tgaattgcaa agaaatgaat acagtcactg gtgcattaag tattat
//
LOCUS       DAZKGS010000272         3527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKGS010000272 DAZKGS010000000
VERSION     DAZKGS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..303
                     /locus_tag="ACHOQY_004783"
                     /pseudo
     CDS             1..303
                     /locus_tag="ACHOQY_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            658..1224
                     /gene="slp"
                     /locus_tag="ACHOQY_004784"
     CDS             658..1224
                     /gene="slp"
                     /locus_tag="ACHOQY_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9680708.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1380..1910
                     /locus_tag="ACHOQY_004785"
     CDS             1380..1910
                     /locus_tag="ACHOQY_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9680709.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(1952..2599)
                     /locus_tag="ACHOQY_004786"
     CDS             complement(1952..2599)
                     /locus_tag="ACHOQY_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9680710.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(2663..2989)
                     /gene="hdeB"
                     /locus_tag="ACHOQY_004787"
     CDS             complement(2663..2989)
                     /gene="hdeB"
                     /locus_tag="ACHOQY_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9680711.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            complement(3105..3437)
                     /gene="hdeA"
                     /locus_tag="ACHOQY_004788"
     CDS             complement(3105..3437)
                     /gene="hdeA"
                     /locus_tag="ACHOQY_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9680712.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         1083 a          748 c          669 g         1027 t
ORIGIN      
        1 aacaaatcga aggtttattt tcaacattat taaccattaa tgacaacttt ttacgagcaa
       61 aagcggaaat tgcttcgaca ataattaaat ttttagaggc caggataacc aatctttcat
      121 acaatgacat attaacatat cagcaagaat tccaaaaaca atgttataag caagttaaag
      181 cctttacgac actctctcga tacaataaaa ttcaaacatg ggcagaaatg tctgaatatc
      241 agttcgaagt atttcaatac gagacgctta accccaaaaa aatgtcacac acgccttatt
      301 taaagaggcc acttcctaat gaaaaagata taaattatgg ggtggaaata gaaataccat
      361 ctggcaaaag aattcgctta tcaaatcatt atcagaatat aataccataa tattgattat
      421 tagcacttta taattattga ataaaaatag attttatgta attttaaaac aaaggattat
      481 attatggggc gatggatact catgccacgc cccaaaatca atggaattga actaaacaaa
      541 agggatataa acatcagaca ggtttacgtt actatcaggc atatcacctc agaatcagat
      601 gaaaactata aagaaatatc tattatggct ttaatatttg ttgataagga tagtaacatg
      661 aacatgacaa aaggtgcact catcctcagc ctttcatttt tgcttgccgc atgtagttca
      721 attccgcaaa atatcaaagg caataaccaa cctgatattc aaaaaagttt tgttgctgtt
      781 cataaccagc cggggttata tgttggtcaa caagcgcgct ttggtgggaa ggttatcaac
      841 gttatcaatg gcaaaacgga tacgttgtta gaaatcgctg tattaccgtt ggatagctat
      901 gcgaagcctg atattgaagc caactatcag ggccgactgc tcgccagaca aagcggcttc
      961 cttgatccag tgaactatcg taatcacttt gttaccatcc tcggtaccat tcagggtgaa
     1021 caaccaggct ttatcaataa agtcccgtat aacttcctgg aagtgaatat gcagggcatc
     1081 caggtgtggc atttgagaga agtggttaat accacctata acctgtggga ttacggctat
     1141 ggtgcattct ggccggaacc gggctggggt gcgccttact acaccaatgc ggtgagtcag
     1201 gtaacacctg agctggtcaa ataacaccac cgaaagatgc agatgctttc tcagcatctg
     1261 catcatgtat tacataaaat taatatacag taagctaact attattatta taagccctgt
     1321 cctgttaatt acctttggca aactgattat aaagttaatg tccgcaccag gagtcggtta
     1381 tgtttcttat aattaccagg gatacgatgt tcttcaccgc gatgaaaaac attctgagta
     1441 aaggtaatgt cgttcatata cagaacgaag aagagatcga cgtaatgttg catcagaatg
     1501 ccttcgtcat tattgataca ttaatgaata atgtatttca ttctaatttt ctcactcaaa
     1561 ttgaacgatt aaaacctgtc catgtcattg ttttctcccc ctttaatatt aaacgctgtc
     1621 tggggaaagt accggtgacc tttgttccgc ggactatcac tatcattgat tttgtcgcac
     1681 tcatcaatgg cagttactgc tctgtgcctg aagcgaatgt gtcactttcg cgcaagcaac
     1741 atcaggttct gagctgcatt gcgaatcaaa tgacaactga agatattctg gagaaactga
     1801 aaatatcgct aaaaacgttc tactgccata aacacaatat catgatgatc ctcaatctta
     1861 agcggatcaa tgagctggta cgccatcagc atattgatta tctggtgtga atttcaggct
     1921 tacggtgagt ctggctacgc tgccacacag attagctaat tgaaacgcct ttcacccctg
     1981 ctataccttt taataatcgc aacaggtctt ctatcgacgt tgtggcatga agctggatat
     2041 caatggcgac cacttcatga tcttcatttt cctgcaacga aactaaatcc gtttttattt
     2101 tttgctgctt aaaccagtcg agcattgata ccgtattacc gttcactaat gtcagctgta
     2161 aatggtaatt tttgttcatc aggcggaagg ttaattgatg gaagacttcc agcaccaaca
     2221 aggtcatcac tgaaccataa atacccagtt cgtacatacc gctgccaata accataccta
     2281 tggcggcggt cacccagata tccgctgccg tcgtcagacc tacaatattt tggttacgaa
     2341 cgagaatgtt acctgcaccg ataaacccga cgcccgtcac cacctgagca gcaatacggc
     2401 tggggtcgag tccgacgtga tctaaagaca gcacgtcagc aaaaccatat ttcgaaacaa
     2461 tcataaacag ggcgcttccc atgccaatta atacatgtgt gcgtaaccct gctcctttgc
     2521 cgcgcatttg cctttccatg ccaatagcgc cacaggcaat tgccgcgagt ataagacgta
     2581 taataaattc tgctgtcata taaaaatgca aaaaggagca gcaagatggc tcaacttgct
     2641 actccttttt acttgtacct cattaattcg gcaagtcatt agatgcttga tttttgaagg
     2701 tatacaaatt tttctgcggg tttttcttac agtattcgat cactttagga atttgagtga
     2761 gatcggtttc atttaaagta acggtatcgc cacctttata tactgtttct tcatgcagca
     2821 tccaccatgc aaccggggtc attgcttttg gattcagatc aataaattcc tggcaggtca
     2881 tatctttagc ggattcattg gctgccagcg cagattgtgc gttcaccagt gacaaagccg
     2941 ctacagcgcc cataaaaata aacgctttac ggagagatga aatattcatt ttgtaaccca
     3001 ttcaatatag agattatata tgaacaggag gaatagctgc ttttaaagaa gatattccgg
     3061 cctgttcaat atgcaaggaa gtacgatgta acgcattccc ggaattacat atctttctta
     3121 attttgtccc attcgccttt aactttatct ttaaagttgg cttgtttatc ctgagtacaa
     3181 gcctgaacga tagctggggt tacggttgca ataccctgaa catctaaaac cgcatcttct
     3241 ggtttatctt tgttgttcag cgcttcagca aaaccaactg cagttggctg gaaggattcg
     3301 tccacagcca ggaaatcttc acaggtccag gagttgaccg gttttttgtt atcagctgct
     3361 ttttgcgcat ccgctgcatt gctcacaact ggcagaagaa gcagaccacc aagaataacg
     3421 cctaatactt ttttcatcgt aatatcctca actataaagt gaaagagccg tcacgaatca
     3481 atttcgacac tgaggttata acctggtttt ctgtatatgt catgttg
//
LOCUS       DAZKGS010000273         3526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKGS010000273 DAZKGS010000000
VERSION     DAZKGS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHOQY_004789"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHOQY_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9680713.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHOQY_004790"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHOQY_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9680714.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..2738)
                     /locus_tag="ACHOQY_004791"
                     /pseudo
     CDS             complement(1944..2738)
                     /locus_tag="ACHOQY_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(2780..>3526)
                     /locus_tag="ACHOQY_004792"
     CDS             complement(2780..>3526)
                     /locus_tag="ACHOQY_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFP9680715.1"
                     /translation="DWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
                     VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTER
                     LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNN
                     RRLLGRLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          925 a          975 c          829 g          797 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg
     1261 ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggattata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaagattg aaccgccccg ggttcctgga
     2761 gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa gcataataag
     2821 ctttttctgc ttctgccgga ggagtatggc ccagccttcc cagcaatcgt cgattgttat
     2881 accagtccac ccacgttagt gtggccagtt ccacttctgc acggtttttc cagctcttac
     2941 ggtgtattac ctccgctttg taaagaccat tgatgctctc agccatcgcg ttgtcatacg
     3001 agtcgcctgt actccctgtt gatgccagta atccggcttc ttttagtcgc tccgtatagg
     3061 ccagtgacac atactgagag cctttatcgc tgtgatggat ggtgccagac ggacgacggg
     3121 cccacaacgc ctgctccagc gcatccagca cgaatgtcgt ttccatagac gatgagaccc
     3181 gccaccccac gatgtatccg gcaaacacat caatgataaa cgccacatag acgaagccct
     3241 gccatgtgct gacgtaagta aaatcagcca cccacagctg gtcaggtcgt tctgccacga
     3301 actgacggtt tacgcggtcg cctgcggcaa cggctttccg gctgatggtc gtacggacct
     3361 ttttaccccg gagaacaccg gcaagtccca taaccgccat gagacgtgcc actgtacatc
     3421 tggccaccct gattccttcc cgtaacaact gacgccagac tttacgcaca ccgtacacct
     3481 gatgattttc atcgtatacg cgctgtatct ctctcttcag ccagtc
//
LOCUS       DAZKGS010000274         3464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKGS010000274 DAZKGS010000000
VERSION     DAZKGS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..420
                     /locus_tag="ACHOQY_004793"
     CDS             <1..420
                     /locus_tag="ACHOQY_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein E"
                     /protein_id="HFP9680716.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIRCYGAIQDAQALSEGVVASSRY
                     PKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            472..855
                     /gene="gpFI"
                     /locus_tag="ACHOQY_004794"
     CDS             472..855
                     /gene="gpFI"
                     /locus_tag="ACHOQY_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9680717.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            848..1201
                     /locus_tag="ACHOQY_004795"
     CDS             848..1201
                     /locus_tag="ACHOQY_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9680718.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1216..1749
                     /locus_tag="ACHOQY_004796"
     CDS             1216..1749
                     /locus_tag="ACHOQY_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680719.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1746..2141
                     /locus_tag="ACHOQY_004797"
     CDS             1746..2141
                     /locus_tag="ACHOQY_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9680720.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2149..2901
                     /locus_tag="ACHOQY_004798"
     CDS             2149..2901
                     /locus_tag="ACHOQY_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680721.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            2915..3346
                     /locus_tag="ACHOQY_004799"
     CDS             2915..3346
                     /locus_tag="ACHOQY_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9680722.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            3373..>3464
                     /locus_tag="ACHOQY_004800"
     CDS             3373..>3464
                     /locus_tag="ACHOQY_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9680723.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYF"
BASE COUNT          804 a          794 c         1098 g          768 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcg aaaacgtctt atgtggcaga ggacggtacc
      181 gaaaaacgtt atctgccgga gggcacgctg gtcctgggaa atacggctgc agatgggatc
      241 cgttgttacg gtgccattca ggatgcgcag gcgttgtccg aaggtgtggt ggcttcttcc
      301 cgttacccga aacactggct gaccgtgggc gatccggccc gtgaatttac catgacgcag
      361 tccgcaccgc tgatggtgct gccggatccg gatgagtttg tggtggtgca ggtgaaataa
      421 tccgtgagcg ggggcgaaat gcccccgtgt cttttttcac agggggctga tatggcaaca
      481 aaagaagaaa atcagaaaca tcttcgtcaa ctggctggcc tgctggggcg cgaggcggat
      541 atgtcgggga gtgctgcgga tattgcgcaa cgtgtgtctg agtgggaaga ggagcttgct
      601 gcttcccggg agggcattat gcctggtgat gagagcgggc ctgagcaaaa tcacacagac
      661 gatggtgagc agttgcacaa cactgatgct acggatgatg ttaaagcggt ccgtgtgcgg
      721 aaatgcctgc atgtgatggg gtattgcccg gagacaggcc gtcccgttga actgacgtac
      781 cggggcatgc gtgttctggt gccatcacca ctggcgacag ccatgataca gcacggaacg
      841 gctgagcatg cgtgattttc agaatgcctt tgatgctgcc cttgccgggg tggacagcac
      901 gattgttgaa gtgatggggc tctgtgcgca gttcacctcg ggggcacagt gtggcagcga
      961 agttcagggg gtttttgacg atccggagtc gctggggttt gccggtagcg gggtccgtat
     1021 tgaaggaagc tgcccgtcat tatttgtacg gacggatacg gttcgtgctg tgcggcgtgg
     1081 tgacacgctg accattaatg gtgagacatt ctgggtggat cgtgtttctc cggatgacgg
     1141 gggcagttgt tatctctggc tcaaccgtgg gcaaccaccg gcagttaacc ggcgacgata
     1201 aacgcagggt gaattatggc gataaaaggg cttgatcagg cgattgaaaa tctgagccgg
     1261 gttcgtaaaa acgccattcc ggcggcttca gcaatggcca ttaaccgcgt ggccacaacg
     1321 gcgattaatc agtcttcgtc acaggttgcc cgggagacca gggtgagccg gaaactggta
     1381 aaggaacggt ccagactgaa acgggccacg gtcagaaatc cgaatgccag aattatcgtt
     1441 aaccgcggtg atctcccggt gattaagctg gggatcagga tgccggggcg tcgtccggac
     1501 agcatactca aagccggtca gcatcggtat cagcgggcat ttattcagcg attaaaaaat
     1561 ggtcgctggc atgtcatgca gcgtgtggcc gggaaaaacc gttaccccat tgatgtggtg
     1621 aaaatcccga tggcggcccc actgaaacag gcgtttgatg agaatgttga ccgtatccgg
     1681 cgtgaacgcc tgcccggaga actggcatat gcgctgaaac aacaactgag gattgcgata
     1741 aaacgatgaa acatactgat atccgtgctg cagtgctgga tgcactggag ctgcatgaac
     1801 acggggcgac gctgtttgat ggtcgccccg ttgtttttga cgaagaggat tttcccgcgg
     1861 tcgcggttta tctgacggat gcagagtata ccggtgaaga gctggatgcg gatacctggc
     1921 gggccacact gcatattgaa gttttcctgc ctgctcaggt gccggattca gagctggatt
     1981 cgtggatgga gtcccggatt tatccggcga tgtccgcgat ccctgcactg gcagggatga
     2041 ttaccacgat ggttcagcag ggctatgact atcgtcgtga tgacgatatg gcgttatgga
     2101 gctctgcaga tttgacttat tccattacat acgagatgtg aggacgatat gccaacacca
     2161 aatcccctgg cgccggtaaa aggtgccggt accaccctgt gggtttataa cggtcagggt
     2221 gatgcttatg caaacccgtt gtcagatgat gactggcagc gactggctaa ggtgaaggat
     2281 ctgacgccgg gcgagatgac ggcagaatcc tacgatgata actacctgga tgatgaagac
     2341 gcggactgga gcgcgaccgg gcagggacag aaatctgcag gtgataccag ttttacactg
     2401 gcctggaaac cgggagaaga aggtcagaaa gggcttatag gctggtttga aagcggggat
     2461 gtgcgggcct ataaaatccg ttttccgaat ggcacggtgg atgtgtttcg tggctggatc
     2521 agcagtatcg gtaaggcagt gacggcgaaa gaagtgatca cccgaacagt gaaagtgacc
     2581 aacgtgggca aaccttccgt ggcggaagaa cgcagcgaaa ttacgccggc cactgcaatt
     2641 aaggtgacac cgacatccgg taccgtggaa aaaggaaaaa caaccaccct gactgtttct
     2701 tttgagccgg aaagtgcaac cgacaagacg ttcagagcgg tttccgccga tccgtcaacg
     2761 ggaaccattg ctgtgaaaga tatggtgatc actgtgacgg gggttaaggc tggaaaagtg
     2821 agtatccccg tgatttccgg taatggtcag tttgccacgg tagctgaagt caccgttact
     2881 gaagcgggcg ctgcagggta aacggaggtc atacatgttt ctgaaaacag aacaatttga
     2941 atataacggt gtgtctgtca cgctttccga attgtctgcg ctgcagcgta ttgagcatct
     3001 tgccctcctg aaacggcgtg cagaacaggc agaatccagc ggcaacctgc aggtaagcgt
     3061 ggaagatctc gtcagaaccg gcgcgtttct ggtggcgatg tccctgtggc ataaccatcc
     3121 gcagaaaacg gggtcaccgt caatgaatga ggccgtgatg cagattgagc aggaagtcct
     3181 gaccacctgg cctgctgatg ccattgcccg ggcggaagat gtggtgttgc gtctgtccgg
     3241 gatgagcggg cctgttcatg tggatacgga tattaccgaa gtggcgaaaa ataacgcgct
     3301 gactgatgat gatttttctg cgggaaagtc ttcgacggcg agctgaattt tgccctcaga
     3361 ctggcgcgtg agatggggag gcctgactgg cgcgccatgc ttgccgggat gacatccacc
     3421 gaatatgccg actggcgaca tttttaccgc atgcattatt ttca
//
LOCUS       DAZKGS010000275         3437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKGS010000275 DAZKGS010000000
VERSION     DAZKGS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..692
                     /gene="yehC"
                     /locus_tag="ACHOQY_004801"
     CDS             18..692
                     /gene="yehC"
                     /locus_tag="ACHOQY_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9680724.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            708..3188
                     /gene="yehB"
                     /locus_tag="ACHOQY_004802"
     CDS             708..3188
                     /gene="yehB"
                     /locus_tag="ACHOQY_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9680725.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3202..>3437
                     /locus_tag="ACHOQY_004803"
     CDS             3202..>3437
                     /locus_tag="ACHOQY_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFP9680726.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDT"
BASE COUNT          982 a          635 c          864 g          956 t
ORIGIN      
        1 atttaagagg cattaagatg aaaggattat tatctttact cattttttct atggtccttc
       61 ctgcacatgc cggaattgtt atctacggga cgcgcattat ttacccggca gaaaataaag
      121 aagttatggt gcagttgatg aaccagggaa accgttcttc gctgctgcag gcgtggattg
      181 atgatggcga tacgtcgtta ccaccagaaa aaattcaggt tcctttcatg ttaacgccac
      241 cagtggcaaa aataggggca aattccgggc agcaagtaaa aatcaaaatt atgccgaata
      301 aactgcccac taataaagaa agcatttttt atctgaatgt tctggatatt ccaccaaata
      361 gtccagagca agaaggcaaa aatgcactga agtttgcgat gcaaaacaga attaagttgt
      421 tttaccggcc agcgggtatt gctccggtaa ataaagcgac ctttaaaaag ttactggtaa
      481 atcgcagtgg caatggattg gtgataaaaa atgactcagc taattgggtg acgatttcgg
      541 atgtcaaagc taataatgtt aaagtcaatt atgaaactat tatgattgcc cccttagaaa
      601 gtcagagtgt taatgtcaaa agtaataatg caaataactg gtatctgacc attatcgatg
      661 accacggcaa ctatattagt gacaaaattt aattgcagga gctgcctatg ttgagaatga
      721 ctccacttgc atcagcaatc gtagcgttat tgctcggcat tgaagcttat gcagctgaag
      781 aaacctttga tacccatttt atgataggtg gaatgaaaga ccagcaggtt gcaaatattc
      841 gtcttgatga taatcaaccc ttaccggggc agtatgacat cgatatttat gtcaataagc
      901 aatggcgcgg gaaatatgag attattgtta aagacaaccc gcaagaaaca tgtttatcaa
      961 tagaagttat caagcggtta ggcattaata gcgataactt cgccagcggt aagcaatgtt
     1021 taacatttga gcaacttgtt cagggtggga gctatacctg ggatatcggg gtttttcgtc
     1081 tcgatttcag tgtcccgcag gcctgggtgg aagaactgga aagtggctat gttccaccgg
     1141 aaaactggga gcggggtatt aatgcgtttt atacctctta ttatgtgagt cagtattaca
     1201 gtgactataa agcgtcgggt aatagtaaga gtacatatgt acgttttaac agcgggttaa
     1261 atttactggg gtggcaactg cattctgatg ccagtttcag taaaacaaat aacaatccag
     1321 gggtgtggaa aagcaatacc ctgtatctgg aacgtggatt tgcccaactt ctcggcacgc
     1381 ttcgcgtggg tgatatgtac acatcaagcg atatttttga ttctgttcgc ttcagcggtg
     1441 tgcggttgtt tcgtgatatg cagatgttgc ctaactcgaa acaaaatttt acgccacggg
     1501 tgcaggggat tgctcagagt aatgcgctgg taactattga acagaacggt tttgtggttt
     1561 atcagaaaga ggttcctcct ggcccgttcg cgattacaga tttgcagttg gccggtggtg
     1621 gagcagatct tgatgtcagc gtgaaagagg cggacggctc ggtaaccacc tatctggtgc
     1681 cttatgcagc agtgccaaat atgctgcaac ccggcgtatc gaaatatgat tttgcggcgg
     1741 gtcgtagcca tattgaaggg gcgagcaaac aaagtgattt tgtccaggcg ggttatcagt
     1801 atggttttaa taatttattg acgctgtatg gtggctcgat ggtcgcgaat aattattacg
     1861 cgtttacttt ggggactggc tggaatacac gcattggtgc catttccgtc gatgccacga
     1921 agtcgcatag taaacaagac aacggcgatg tgtttgacgg gcaaagttat caaattgcct
     1981 acaacaaatt tgtgagccaa acgtcgacgc gttttggtct ggcggcctgg cgttattcat
     2041 cgcgtgatta ccggacattt aatgatcacg tttgggcaaa caataaagat aattatcgcc
     2101 gtgatgaaaa cgatgtctat gacattgccg attattacca gaacgatttt ggccgtaaaa
     2161 atagcttctc tgccaatatg agccagtcat tgccagaagg ctgggggtct gtgtccttaa
     2221 gtacgttatg gcgagattac tgggggcgta gcggcagcag taaggattat cagttgagtt
     2281 attccaataa ctggcggcgg ataagctata tcctcgcggc aagccaggcg tatgacgaga
     2341 atcatcatga agagaaacgt tttaatattt ttatatcgat tccctttgat tggggtgatg
     2401 acgttacgac gcctcgtcgg caaatatata tgtctaactc aacgacgttt gatgatcagg
     2461 ggtttgcctc aaataatacg ggattatcag gaaccgttgg aagccgggat cagtttaact
     2521 atggggtcaa cctgagttat cagaatcagg gaaatgaaac gacagctggg gcgaatttga
     2581 cctggaacgc gccggttgcg acagtgaatg gcagttatag tcagtcgagt acttatcgac
     2641 aggctggagc cagtgtttca gggggcattg tcgcctggtc ggggggcgtt aatctggcga
     2701 accgtctttc cgaaacgttt gctgtgatga atgcgccagg aattaaagat gcttatgtca
     2761 atgggcaaaa atatcgcaca acaaaccgta atggagtggt ggtatacgac ggaatgacac
     2821 cttatcggga aaatcacctg atgctggatg tgtcgcaaag cgatagcgaa gcagaattac
     2881 gtggcaaccg gaaaattgcc gcctcttatc gcggcgcggt tgtactggtt aattttgata
     2941 ccgatcagcg caagccctgg tttataaaag cgttaagagc ggatgggcaa ccattaatgt
     3001 ttggttatga agtcaatgat atccatggtc ataatattgg cgttgtcggt cagggaagcc
     3061 agttatttat tcgcaccaat gaagtaccgc catcggttaa tgtagcaatt gataagcaac
     3121 aaggactttc atgcacaatc accttcggta aagagattga tgaaagtaga aactatattt
     3181 gccagtaata gtcaggtggt tatgaatatt cgtataatgt tatttatagt aatgatgatg
     3241 gttatgcctg tgagctatgc ggcatgttat agtgagttat ctgttcagca caatttggtt
     3301 gttcaggggg attttgcact tactcaaaca caaatggcga catatgagca taattttaat
     3361 gattcgtcat gcgtgagtac aaatactatc acccctatga gcccgtcgga tattattgtt
     3421 ggactttata acgatac
//
LOCUS       DAZKGS010000276         3427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKGS010000276 DAZKGS010000000
VERSION     DAZKGS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(17..430)
                     /gene="hiuH"
                     /locus_tag="ACHOQY_004804"
     CDS             complement(17..430)
                     /gene="hiuH"
                     /locus_tag="ACHOQY_004804"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9680727.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            563..1234
                     /gene="hprR"
                     /locus_tag="ACHOQY_004805"
     CDS             563..1234
                     /gene="hprR"
                     /locus_tag="ACHOQY_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9680728.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1234..2592
                     /gene="hprS"
                     /locus_tag="ACHOQY_004806"
     CDS             1234..2592
                     /gene="hprS"
                     /locus_tag="ACHOQY_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9680729.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(2700..>3427)
                     /gene="hchA"
                     /locus_tag="ACHOQY_004807"
     CDS             complement(2700..>3427)
                     /gene="hchA"
                     /locus_tag="ACHOQY_004807"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9680730.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT          975 a          680 c          787 g          985 t
ORIGIN      
        1 tttacatatt tatgcattaa ctgccacgat aggttgaata cccatattgg ctaagtaata
       61 aaggcacatg ataatgctcg ttcactttat taatatgaaa ttcaacgggg atctcaggga
      121 agaaactttc aagattttgt ttcttgaaat agtccccggt tttaaatacg acacggtaat
      181 cgcccgtagt tgcagtttgc tcgggccaca gtgccttaat tcgtccatcc ttatctgttt
      241 tggcggtatt aagttgtaac cagccgttgt ccgctttctt ttcaagagtg actgtcacgt
      301 cggcagcggg ttttcctgtt tgctggttca aaatgtgcac actaagaatg ttttgttgtg
      361 cagcatagac caaagaaggt aatgaaaatg cagccgttac tacggagagt actaaataac
      421 gctttaacat gtttatatcc ttgtcatgtg aatgagtttt gaggatagcg aaaaagcgtc
      481 cacggtaacc ttacagcagc attacaattt tgtaatgaac caggctgttt ctataacata
      541 tgatttatgg catattattt tcatgaagat tttacttatt gaagataatc aaaggaccca
      601 ggagtgggta acgcaggggc tttccgaagc gggttatgtc attgatgccg tttctgatgg
      661 cagagatggg ctttatcttg cgcttaagga tgattatgca ttgatcattc tggatattat
      721 gcttccgggt atggatggct ggcagatctt acaaacgtta agaacagcaa agcaaactcc
      781 tgttatttgc cttactgcaa gagattctgt cgatgacaga gtcagagggc tggacagtgg
      841 ggcaaatgat tatctggtaa aacctttttc attttctgag ttgctggcaa gggttcgggc
      901 acaattaagg caacatcacg ctttgaattc aacattagaa atcagtggct taagaatgga
      961 ctctgttagt caaagtgtga gcagggacaa tatcagtatt acactgacgc gcaaggagtt
     1021 tcagttactt tggctactgg cctccagagc tggcgagatt atccccagaa cggttattgc
     1081 gagtgaaatt tggggaatca actttgatag tgataccaat acggtggacg tcgccattcg
     1141 caggctccgc gcaaaagttg atgatccttt tcctgaaaag ctaatcgcca caatccgggg
     1201 gatgggctat tcattcgtag cggtaaaaaa ataatgaaaa gattatctat aaccgtccgt
     1261 ttaaccttgc tttttatatt gctgctatct gttgctggcg ctggaattgt atggactctc
     1321 tataatggcc tggcaagtga gttgaaatgg cgcgatgata caacactcat taaccggaca
     1381 gcgcagatca agcagttgtt aattgatggg gtaaatccag atacgttacc tgtgtacttt
     1441 aaccggatga tggatgttag tcaggatatc ttgatcattc atggtgatag catcaacaaa
     1501 attgttaacc ggacgaatgt cagtgatgac atgttaaata acatacctgc tactgagaca
     1561 atcagcgcag ctggcattta cagaagcatt attaatgata cagagataga tgctttacga
     1621 attaatattg atgaagtttc gccatcatta acggttactg tggctaaatt ggcttcagcc
     1681 agacataaca tgcttgaaca gtataaaatt aatagcatta taatttgcat tgtcgccatt
     1741 gtactttgct cagtattaag tccgctgtta atcagaacgg gattacgaga gatcaaaaag
     1801 ttgagtggtg taacggaagc gctgaattat aacgatagcc gggagcctgt tgaggttagc
     1861 gcattaccga gagaactaaa acctcttggg caggcgttga ataaaatgca tcatgcttta
     1921 gtcaaagatt ttgagcgtct aagtcagttt gctgacgatc tcgctcatga acttagaacg
     1981 cccattaatg cattactggg tcagaatcag gttacgctca gtcaaaccag aagtatcgct
     2041 gaatatcaaa aaacaattgc cggtaacatt gaagagctgg aaaatatttc gcggttaaca
     2101 gagaacatac tgtttcttgc ccgggcagat aaaaacaatg ttttggtgaa actggactca
     2161 ctttttctca ataaggaagt cgaaaatttg ttggattatc ttgaatacct ttcagacgag
     2221 aaagagattt gctttaaggt cgagtgcaat cagcaaatct ttgcggataa aattttactg
     2281 caacgaatgt tatcgaatct tattgttaat gccattagat attcaccaga aaaatcgcgc
     2341 attcatataa ccagttttct tgataccaac ggctatctta atattgatat cgccagtcct
     2401 ggaacgaaaa ttaatgagcc tgaaaaactc ttccgtagat tttggcgggg agataattcg
     2461 cgtcattccg aaggtcaggg acttggtctt tctttagtca aagcgattgc cgaattgcat
     2521 gggggaagtg ctacgtatca ctattttaat aagcataatg tgttccgaat tacgttaccg
     2581 caaagaaatt aacatagggc ttcagtacgc cataaatcaa ccatgaaaaa acgcagccac
     2641 gtcgtgagta ttaacgtggc tgcgattgat tatgcgctta cattcatacg taacagggat
     2701 taacccgcat aagctgccag catttcctgc gccgccagtt tacccaacgc attcgctgcg
     2761 aaaggagtgt cgccggtgag aactttacgg tccttatgta ctcgcccggt gatgtcgtca
     2821 ttaataatgt tcatgcccat tttcttcagc tcttcgccga agtaccaggt gagatgaccc
     2881 ggcatatagc ctatctctgg cgtttgtttg tctgcggcgt ctgggaatgc gcaaatggaa
     2941 taaccattca gtgggttatc gccgtggcga agcgccagaa aagccgccgg gccgtggcaa
     3001 agggagataa caaaacggtc atttttgatt gcccactgca aagcagcagc cacgtcctgg
     3061 ctttctggta aaccaataag tgcgccatga ccaccaggaa caaagattgc tgcatattcg
     3121 ctgtctgcgt tgaggctgac gacaacatcc gcgatttttt tcggattgcg gaacaacgat
     3181 ttatgctgct caaagaatgg cattactttt tcatctttgt gcggcatagc ccagtattca
     3241 aacttggtca tcagaccgga aatggtcgcc acttcgaatt cgaaacctgc agcatggaga
     3301 tgatacaacg gcagcaacgt ttcaatcgga tggttaccgg tcgagaacag ttttccgtta
     3361 tcggtcggca aataacgttc gtccgcggcg atcaccagaa ttttatgttt accgcgatac
     3421 ggttttg
//
LOCUS       DAZKGS010000277         3426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKGS010000277 DAZKGS010000000
VERSION     DAZKGS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>3426
                     /gene="toxB"
                     /locus_tag="ACHOQY_004808"
     CDS             <1..>3426
                     /gene="toxB"
                     /locus_tag="ACHOQY_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFP9680731.1"
                     /translation="ISGASLEGFISSGLEVCASRIGGTAGRYLSNVAKVIKLPLLDIG
                     INIWSLYDSSLNHARATTQIEYISTAIDVSFSSINTALSIGAIAYPPLAIAIVPITIF
                     SHEVKNYAVYVNQINERHKLWLEAEKYLDNGSAKVLSINKDTGIIDLSNNQVLGNVHL
                     DMRENPPILHGEKSYNSGKNIGSHPDMTDREIMESGAYNFACTKLSDAGEPDIFGWGD
                     KDVCNSMELSESQLANGYSNRQWPSQSPVIPEGIYNTVYLGYGETLRANTEVTLSSTG
                     YFYEIARAYTDDELSEPLLTVCNQRSSVIGGKEPLTIIIPALEHGMLGSNLHMIERFK
                     NYNFSISGGKGGIKLMVGGIGDYNIECTPGVRNIISFEQLSRDFNLDLDLSDERKQNF
                     YFHHPSGFYSGKVMSITQKGINSVVGTKQGYDKIRGNNLDNTFSLGNGGGVIYSGGGS
                     NTYFVPATLQHNLHIYISEKSNGNHIILGETHSRLSIECHFSDDKREFIKMDLYNGGD
                     VILESDTLQKINTFSKNITIQTADGVMANWDDESSTLSVYSIDMIAWRDKNKTEKEPL
                     PLDVIQLTNWRIHNSCSLFYDKYQVDIEANKLTYTILFPDTELPVQLNYASVIYGNHG
                     AKYTFLDSGSKAITIHITDESGDYDTFDFRNIVFEHHTNDMFISFDNQGGFVISISNN
                     ATNELTDINVFRKNMTSFDGCRSLIYLPSGDVFYISDIYKMSRGRKSVKLNVEKKPDI
                     NDIINVAILETSYLQIKKIPNNDDGSYVLCLDNPNLSSYTLHFNDLSGYISSIWDNIR
                     GSFTPFHNNTVNIAPNEKKFISLTEPDKLSFNIDVFREALEVKNSYKLSKFTWETYGD
                     IVVSPEDRISHLELDGFNYFSQPELDAQISDTFSYLYDNFQIADGDVHIKLLHLDRET
                     KQITPHRIILQKYFIDSFAKTSISDRENNIYPVICDSPDHFTNDIYRHPFRIVLGNKT
                     LYPSEELIKFIRTSKEYLSNMDIINNIIVPQKTIKKNKLSVVSLNSNLKNEVVLSGVM
                     TGTSKVFHLNKSGDLLLTTSKTHGGGVIVIFKDFINNWWKYNLTLITVPIDDKLSDNR
                     INITPMGITIHEIVNGDDKLFFYPTPLKNGGFILHNPLYTNSFPSSYSHELFD"
BASE COUNT         1202 a          514 c          607 g         1103 t
ORIGIN      
        1 gtatatctgg tgcctcgctt gaaggattta tatcttctgg tttagaagtg tgcgcgtcaa
       61 gaataggtgg caccgctggg cgttatctta gcaatgtggc aaaagtaata aaattacctt
      121 tgctagatat aggcataaat atttggtcat tatatgactc ttcattaaat catgccagag
      181 ccaccacaca aattgaatat atatcaacag cgatagatgt atctttttca tctataaata
      241 ctgctttatc aataggcgca atcgcatatc cacctttggc aatagctata gttccgataa
      301 ctatcttttc tcatgaagta aaaaactatg ctgtttatgt gaatcaaatt aatgagcgac
      361 ataagttatg gcttgaagct gaaaaatatc tggacaatgg aagcgcaaag gttctaagca
      421 ttaacaaaga tacaggcata atcgatttat caaataatca ggtgttaggt aatgttcatt
      481 tagatatgag agaaaaccct ccgatattac atggtgaaaa atcatataat agcgggaaga
      541 atattggaag tcatccagat atgactgaca gagaaattat ggagtctgga gcatacaatt
      601 ttgcatgtac aaaactctca gatgcaggtg aacccgatat tttcggttgg ggagataagg
      661 atgtatgtaa ttcaatggaa ctgtctgaat ctcaattagc taatgggtat tctaaccgcc
      721 aatggccttc acaaagtcca gttattcctg aagggattta taatacagta tatcttgggt
      781 atggagaaac attgcgagct aatacagaag ttacattgag tagcactggt tacttttacg
      841 agattgcaag ggcatatact gatgatgaac tatctgagcc attattaaca gtatgtaacc
      901 aacgtagtag tgtaattggt ggaaaagaac cattaacaat aatcattcca gctcttgaac
      961 atggtatgct tggctctaat ctacatatga ttgaacgttt taaaaactac aatttttcaa
     1021 tatcaggagg gaagggcggg ataaaattaa tggttggtgg tattggtgat tacaacatag
     1081 aatgtacccc cggtgttaga aatattattt cttttgaaca attatcacgt gacttcaatc
     1141 tggatttgga tttatcagat gagaggaaac aaaattttta ctttcatcat ccctctggtt
     1201 tttattcagg aaaagttatg agtataacgc aaaagggcat aaattcggtt gttggtacta
     1261 aacagggata tgataagatt cgtggtaata atttagataa taccttttcc cttggaaatg
     1321 gggggggggt tatatattct ggtggaggaa gcaatacata ttttgttcct gctacactac
     1381 agcataattt gcatatatat atatcagaaa aatcaaatgg taaccatatt attttaggtg
     1441 agacacactc tcgcttatct attgaatgcc actttagtga tgataaaaga gagtttataa
     1501 agatggactt atataatgga ggtgatgtaa ttttagaaag tgacacttta caaaaaataa
     1561 atacattttc aaaaaatata acaatacaga cagcggatgg agttatggca aactgggatg
     1621 atgaatcaag tactctgtct gtttattcaa tagatatgat tgcctggagg gataaaaata
     1681 aaactgaaaa agagccttta ccacttgatg tgattcagtt aactaattgg aggatacata
     1741 actcatgctc tttattttat gataaatatc aagttgacat tgaagccaat aaattaacgt
     1801 atacaatttt atttcctgat acggagttac ctgttcaact taattatgct agtgttattt
     1861 atggtaatca tggggcaaaa tatacattcc tcgactcagg aagtaaagct ataaccatcc
     1921 acattactga tgaaagtggc gattatgata cttttgactt ccgcaatatt gtttttgaac
     1981 atcacaccaa tgacatgttt ataagctttg ataatcaagg aggatttgtt atatcaatat
     2041 caaataatgc cacaaatgaa ctgacagata ttaatgtatt ccgaaaaaac atgacttctt
     2101 ttgatggctg tcgttcattg atatatttgc ctagcggtga tgttttttat atttcagata
     2161 tctataaaat gtccagagga agaaaatcgg ttaaattaaa tgtagaaaag aaaccggaca
     2221 ttaacgacat catcaatgtg gctattttag aaacttctta tctgcaaata aaaaaaatcc
     2281 caaataatga tgatggcagt tatgttttat gtctagacaa ccctaacctc tcatcttaca
     2341 cattacactt taatgactta tctggatata tatcaagtat atgggataat attagaggaa
     2401 gcttcactcc ttttcataat aatactgtga atatcgcccc taatgaaaaa aaatttattt
     2461 ccctaacaga gccagataag ttgtcattca acattgatgt ttttagagaa gcattagagg
     2521 taaaaaatag ttataagtta agtaaattta catgggaaac gtatggtgat atcgtggtat
     2581 cacctgaaga tagaatttct catctggaat tagatggatt taattatttt tcacaacccg
     2641 agttagatgc tcaaatatca gacacctttt catatctata tgataatttt cagattgctg
     2701 atggtgatgt tcatattaag cttcttcatc tcgatagaga aacaaagcaa ataacgccac
     2761 atcgtattat ccttcaaaaa tactttatag actcatttgc taaaacaagt atctctgaca
     2821 gagaaaacaa tatatatcct gttatttgtg attcacctga tcacttcaca aatgatatat
     2881 ataggcatcc atttaggata gttctcggga ataaaacatt gtatccatca gaagaactta
     2941 taaagtttat aagaacctca aaggaatatt taagcaatat ggatataatt aataatatta
     3001 ttgttccaca aaaaacaata aagaaaaata aattatctgt cgttagttta aatagcaacc
     3061 tcaaaaatga ggttgttttg tctggagtaa tgacaggaac atcaaaggtt tttcatctaa
     3121 ataaatcagg cgacttattg ttaaccactt caaaaactca tgggggcggg gttatagtta
     3181 tttttaaaga ttttataaat aactggtgga aatataacct tacattgata acagttccta
     3241 ttgatgacaa attgtcagat aacaggataa atataacacc aatgggtata acgatacatg
     3301 aaatagtcaa tggggatgat aagttgtttt tttatcctac tccattaaaa aatggaggtt
     3361 ttatcttaca taatccactt tacactaatt catttccttc ttcatattca catgaattat
     3421 ttgatt
//
LOCUS       DAZKGS010000278         3407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKGS010000278 DAZKGS010000000
VERSION     DAZKGS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..1235
                     /gene="yiaY"
                     /locus_tag="ACHOQY_004809"
     CDS             84..1235
                     /gene="yiaY"
                     /locus_tag="ACHOQY_004809"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9680732.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1400..2938
                     /gene="aldB"
                     /locus_tag="ACHOQY_004810"
     CDS             1400..2938
                     /gene="aldB"
                     /locus_tag="ACHOQY_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9680733.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3157..3360)
                     /locus_tag="ACHOQY_004811"
     CDS             complement(3157..3360)
                     /locus_tag="ACHOQY_004811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680734.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
BASE COUNT          845 a          840 c          907 g          815 t
ORIGIN      
        1 gataattaat aataatgatc atgttttcga atgaaaatcc tcagtaagct gcccaccctt
       61 ttttacactt tcaggagtgt gttatggcat cttcaacttt ctttattcct tctgtgaatg
      121 tcatcggcgc tgattcattg actgatgcaa tgaatatgat ggcagattat ggatttaccc
      181 gtaccttaat tgtcactgac agtatgttaa cgaaattagg tatggcgggt gatgtgcaaa
      241 aagcactgga agaacgcaat atttttagcg ttatttatga tggcacccaa cctaacccca
      301 ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga aaataattgc gatagcgtga
      361 tctccttagg cggtggttct ccacacgact gcgcaaaagg tattgcgctg gtggcagcca
      421 atggcggcga tattcgcgat tacgaaggcg ttgaccgctc tgcaaaaccg cagctgccga
      481 tgatcgccat caataccacg gcgggtacgg cctctgaaat gacccgtttc tgcatcatca
      541 ctgacgaagc gcgtcatatc aaaatggcga ttgttgataa acatgtcact ccgctgcttt
      601 ctgtcaatga ctcctctctg atgattggta tgccgaagtc actgaccgcc gcaacgggta
      661 tggatgcctt aacgcacgct atcgaagcgt atgtttctat tgccgccacg ccgatcactg
      721 acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa cctgccgtta gccgttgaag
      781 atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc ccagttcctc gccggtatgg
      841 cgttcaataa tgcttctctg ggttatgttc atgcgatggc gcaccagctg ggcggtttct
      901 acaacctgcc acacggtgta tgtaacgccg ttttgctgcc gcatgttcag gtattcaaca
      961 gcaaagtcgc cgccgcacgt ctgcgtgact gtgccgctgc aatgggcgtg aacgtgacag
     1021 gtaaaaacga tgcggaaggt gctgaagcct gcattaacgc catccgtgaa ctggcgaaga
     1081 aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa agaagaagat ttcgcggttc
     1141 tggcgactaa tgccctgaaa gatgcctgtg gttttactaa cccgatccag gcaactcacg
     1201 aagaaattgt ggcgatttat cgcgcagcga tgtaatcatc atttccacaa cggctggcaa
     1261 attgtcagcc gctttttcaa ctatctctgt aacccttgcc cgtaaattcg tgatagctgt
     1321 cgtaaagctg ttaccgactg gcgaagattt cgccagtcac gtctaccctt gttatacctc
     1381 acaccgcaag gagacgatca tgaccaataa tcccccttca gcacagatta agcccggcga
     1441 gtatggtttc cccctcaagt taaaagcccg ctatgacaac tttattggcg gcgaatgggt
     1501 agcccctgcc gacggcgagt attaccagaa tctgacgccg gtgaccgggc agctgctgtg
     1561 cgaagtggcg tcttcgggca aacgagacat cgatctggcg ctggatgctg cgcacaaagt
     1621 gaaagataaa tgggcgcaca cctcggtgca ggatcgcgcg gcgattctgt ttaagattgc
     1681 cgatcgaatg gaacaaaacc ttgagctgtt agcgacggct gaaacctggg ataacggcaa
     1741 acccattcgc gaaaccagtg ctgccgacgt gccgctggcg attgaccatt ttcgctattt
     1801 cgcctcgtgt attcgggcgc aggaaggtgg gatcagtgaa gttgatagcg aaaccgtggc
     1861 ctatcatttc catgaaccgt taggcgtggt ggggcagatt atcccgtgga acttcccgct
     1921 gctgatggcg agctggaaaa tggctcccgc gctggcggcg ggcaactgtg tggtgctgaa
     1981 acccgcacgt cttaccccgc tttctgtact gctgctaatg gaaatcgtcg gtgatttact
     2041 gccgccgggc gtggtgaacg tggtcaacgg cgcaggtggg gaaattggcg aatatctggc
     2101 gacctcgaaa cgcatcgcca aagtggcgtt taccggctca acggaagtgg gccaacaaat
     2161 tatgcaatac gccacgcaaa acattattcc ggtgacgctg gagctgggcg gcaaatcgcc
     2221 aaatatcttc tttgctgatg tgatggatga agaagatgcc tttttcgata aagcgctgga
     2281 aggctttgca ctgtttgcct ttaaccaggg tgaagtttgc acctgtccga gtcgtgcctt
     2341 agtgcaggag tcaatctacg aacgctttat ggaacgcgcc atccgccgtg tcgaaagcat
     2401 tcgcagcggt aacccactcg acagcgtgac gcaaatgggc gcgcaggttt ctcacgggca
     2461 actggaaacc atcctcaact acattgatat cggtaaaaaa gagggcgcag acgtgctcac
     2521 cggcgggcgg cgcaagctgc tggaaggtga actgaaagac ggctactacc tcgaaccgac
     2581 gattctgttt ggtcagaaca atatgcgggt gttccaggag gagatttttg gcccggtgct
     2641 ggcggtgacc accttcaaaa cgatggaaga agcgctggag ctggcgaacg atacgcaata
     2701 tggcctgggc gcgggcgtct ggagccgcaa cggtaatctg gcctataaga tggggcgcgg
     2761 catacaggct gggcgcgtgt ggaccaactg ctatcacgct tacccggcac atgctgcgtt
     2821 tggtggctac aaacaatcag gtatcggtcg cgaaacccac aagatgatgc tggagcatta
     2881 ccagcaaacc aagtgcctgc tggtgagcta ctcggataaa ccgttggggc tgttctgata
     2941 taagaagctg gtcgcattgg gtattcattg cctgatgcga cgcttacgcg tcttatcatg
     3001 cctacgggaa cctgaccgta ggccggataa ggcgtacacg ccgcatccgg cataaacaaa
     3061 gcgcacgttg ttacaattta cgttttaaac ctacgtgcgc aataacccag actacgccaa
     3121 cagaagtgcg tttctggggc gtgttttgcc aggagtttac tctcgcgttt cgatggtgag
     3181 cgggcaaacc aggccgtaaa tcaccattgg cgttccgagg ccaatcagca ataaacggta
     3241 gataattgaa ggtttaatac gctttatctg tactgtggat gttttactgg agttattcat
     3301 tagcttggtc aatagttcca ttatttcaat atgataaacc tcgacctgtt gaaaagccac
     3361 gcagcacact tcagaaagta tataaaaaac aatatgccgt tatataa
//
LOCUS       DAZKGS010000279         3402 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKGS010000279 DAZKGS010000000
VERSION     DAZKGS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..688
                     /gene="nleG"
                     /locus_tag="ACHOQY_004812"
     CDS             41..688
                     /gene="nleG"
                     /locus_tag="ACHOQY_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFP9680735.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(825..971)
                     /locus_tag="ACHOQY_004813"
     CDS             complement(825..971)
                     /locus_tag="ACHOQY_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680736.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            1399..1677
                     /locus_tag="ACHOQY_004814"
     CDS             1399..1677
                     /locus_tag="ACHOQY_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9680737.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            2215..2376
                     /locus_tag="ACHOQY_004815"
                     /pseudo
     CDS             2215..2376
                     /locus_tag="ACHOQY_004815"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2360..2425
                     /locus_tag="ACHOQY_004816"
     CDS             2360..2425
                     /locus_tag="ACHOQY_004816"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFP9680738.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            2541..2798
                     /locus_tag="ACHOQY_004817"
     CDS             2541..2798
                     /locus_tag="ACHOQY_004817"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9680739.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(2845..>3402)
                     /gene="nleF"
                     /locus_tag="ACHOQY_004818"
     CDS             complement(2845..>3402)
                     /gene="nleF"
                     /locus_tag="ACHOQY_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFP9680740.1"
                     /translation="SGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQDKLDV
                     MVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGMTIED
                     RDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCLVEGC
                     SDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT         1052 a          727 c          652 g          971 t
ORIGIN      
        1 ccaatatggt tcaataacat agactaaaga gggagaaaat atgcctgtca tattaaactt
       61 ttcgagtgaa agagtattgt ctgaaagtga actggaggct ttacgtcatg tcggaagagt
      121 tagtcagagt gaacagcttg ttgtgagagg ccgcactatg agacttcatc atatttcatt
      181 tatggacagt tttagcgtgg aaccggtttc cggcggactg ttggatcgcc ttagtgcaag
      241 agggcatcgc cttctcgcag aaaatctgga gatacagctt aacagaggcc atactttttt
      301 gcaggcattc cgtttatata tggaacagag ccgtgcaacc ccatgtacgc gccaaaacgt
      361 cagtagtgca atacaaaaca aaattaactc tcatgcattt actgttagtc atcaagactt
      421 ttcctgtcat gaacaacacc tgaattgccc gataacatta tgcattcctg aaactggtgt
      481 ttttgtcaga aatgcaaaaa actcagaaat atgttcatta tatgaccaca atgcactcac
      541 tgaacttatc cgacgcaacg ctccacaccc tctcagccgt gaaccctttg ttccggaaat
      601 gattgtcagc aaagatgaat gccattttaa tctaatagaa caatattttt gcatattagc
      661 cacacaaaat atatgcacta gaatttgact gtatggttcg atttaaatgt tgcaaaatta
      721 attttactag ttattgccat ctatttcata ctccctaatt cttgatacaa tctgtctgtc
      781 aaaatagtag attagatcga acttatatta cagctattta gtttctaaaa tggaattttt
      841 aacgttttat acttcacatt acaatctgta ccataagcct cctcagcgag gttgcctgaa
      901 taccctgtcg tgcccatttt tccatcggac gcagttgtct gctctctcga actgctggca
      961 ataaaaatca accagacagc ttgatgtccg ttacatgcag gactaatcac gattggttac
     1021 cgtttacaaa acttgcgtaa cattggagga gaacgaataa tattctctag ctggtacgcg
     1081 ttccatagcg aaacgggcaa ggtccaacag caagagctga aattcagggg gatttatcag
     1141 tcgatttatt cgccatggat gaatcacgct gtacggttat ggtagctctt agcgacaagg
     1201 cataatcatc agtacaccaa tgtaacaacc gttgaaaaag actcattatt ttatgaacca
     1261 gccatcccat ttccagagtg gtaaatatga tttgttaaag tgtaacagta ctctgcgtag
     1321 cctttttaga ttcatagttt aatgtttcta aatacattaa attgcactat aagagaaaga
     1381 cataatgtga ggataaaaat gccattttca ataaaaagta ttttttcagg acacacttgg
     1441 catcaacctg aaatatctag gcctatagca gataaatcat ctactaaaaa ttgtatctta
     1501 gactcaacaa catgcaatgt agatggcttt acagtgttta acagaagatc ctgtagtttt
     1561 gatatgcgtc ctcccggaag cgcagacaga actccacaac tgagattatc aatatcagaa
     1621 gtcgcatgga tgtcaaaaat aatagagaca gagacaaata atacaaacaa atcatagcta
     1681 ttagcccaga aaagagttgg tacactagcc atgaataact tcctaacaac ttgatttttt
     1741 agttattttt ttcttcattc ttgaaaaagg atgccccgaa agggttccag agttcgattt
     1801 tctgcgcttc tgcaaataat caaggggtta caaagtggtt gcccctttgt ttttagtgct
     1861 cttggtatac ccattggtac atttagcgca ataccatctg gtatcactta aagacacgaa
     1921 aaacaacttt ttatcttttt gtttcgctca aattagtata aaaagccgaa caacaaacaa
     1981 taaaaaacca ttaacatcaa tagattatga tggcttcaat ctaaataata ggctatataa
     2041 tactgcaaga cacaacacat gctgtcacta tgagtcaact atttagatga tattagtgac
     2101 ctgtttcaga gcattagcgc aaggcgattc tttgtcttct tgcactaatt ttttatcata
     2161 aaaatgttcc tagcactggg catcaatatc gcaggtcaga aagagctcct ggggatgcgg
     2221 ctggccgaaa atgaaggggc gaatttctgg ttcaatgtgc tgactgaact gaaaaaccgc
     2281 ggtctgaacg atatcctcat cgcctgtgtg tatggcctga aagaattccc ggaggcccgc
     2341 atccagttat gcatcgtgca tatggtgcgc aacagcatgc gcttcgtgtc atggaaggaa
     2401 tacaaagccg tcactcgcga cctgaaagcg attagcctcc cacagaagag gcaggccagc
     2461 aggcactgga agcgtttgct gcggcctggg actgccgcta tccgcagata agccggtgct
     2521 agctgtcaaa ctggactaac ttggcgacgt ttttcgctta tccggcagat atccgcaaag
     2581 tgatctacac aacgaacgcc atcgagtcgc tgaacagtgt gatccggcat gccatcagga
     2641 aacgcaaggt gttcccgacg gacgagtcag taaaaaaagt ggtgtggctg gcaatccagg
     2701 ccgcgtcaca gaaatggaca atgccgttaa gggactggcg aatggcaatg agccgcttta
     2761 ttatcgagtt tggtgaccgt cttgacggtc acttttaaga aaaggcattt acacagaatc
     2821 ctaaacaggc tcacaggcct ctaatcatcc acattgtaaa gatcctttgt tgtaagtaag
     2881 atctggtacc ctaataatat caaaaggatt aaaatcatca ctgcatcctt ccacgaggca
     2941 tttcattgct cgtagatcac catatagctc tgaagagtga tgcggacata gcagattata
     3001 aactttacat ttcgaatctt ctttgccttg tctactttcc tcaattttta ctttcaatga
     3061 atgcagaaaa aaaagatccc tgatataact atccctgtcc tctatcgtca ttcccgccct
     3121 cagcgctccg acaacctcat cagtattttc atttctcacc tcaaatacac tatttctctt
     3181 atccatcagc ccacttacaa aagcacttag ctcttgaaaa atttcctcaa gctcattatt
     3241 atttctggca catgaatata tagagaccat aacatcaagt ttatcttgta attcaggaga
     3301 aagaaagtga ttatgattaa gctcacttac tgattccgga gtcgaagggt tacctcttcc
     3361 tgatacatac atccatgaat aaagatttgc tgaagaacca ct
//
LOCUS       DAZKGS010000280         3380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKGS010000280 DAZKGS010000000
VERSION     DAZKGS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..567)
                     /locus_tag="ACHOQY_004819"
     CDS             complement(<1..567)
                     /locus_tag="ACHOQY_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9680741.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTD"
     gene            complement(638..>3380)
                     /locus_tag="ACHOQY_004820"
     CDS             complement(638..>3380)
                     /locus_tag="ACHOQY_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9680742.1"
                     /translation="EQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAY
                     SNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCN
                     AYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAP
                     FRYSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRG
                     QAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNS
                     QTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSV
                     WGLKLPTLRQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGV
                     TPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTT
                     ETTYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITAT
                     PHLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIR
                     SVNTVGKSAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEF
                     SKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFID
                     PANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISG
                     RVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTV
                     TVTISDDHNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRE
                     TKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          717 a         1033 c          825 g          805 t
ORIGIN      
        1 gtcagtgcgc cagtcgccac tgccggagcc ttcataagca atatcaatgg ccacggattc
       61 ggtcgggtta aactgcacgc cagctcccca cgccagagac gtgttgctgt ggcgaccgtc
      121 atcacttccg gtcagcacgt cgtgcgtttt ccccttgttg tcagttacgc ggagataatc
      181 cccggagaaa gtcgacacac ggctgtaaga cacacccgcc atcgcatacg cgctgaacca
      241 ttcattcacg cgcacagacg gccccgccat cacgctgaac cagcggttac gcacggaatc
      301 ttcatgccag cggatatcgc tgtaacgggt aagctggcga ttcttgtctc ctgcatagct
      361 gaatgacgtc accagcccca gtgtgtccgt aaactcataa cggtatttca cgttaatccc
      421 gttcagatca tcgctgccgg gaacgttcgt cgaggcatga agataccccg cgctcagcgt
      481 ggactgatgt tcagatgccc atgcagacgc accggatacg gccagacaaa tagctgcgga
      541 caaaatggct gcataaagtt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc
      601 gtcattcctg acgcccttta ttggggttat aaatatttca acgaatactg ataccggaag
      661 ccgctttttt ggtcacaatc accgtacagt cggtgatatt ccctgccccc tgattgcctt
      721 tctggaaaat cttaaactcc agagtgacgc taccaccacc actaggcata tcaataactg
      781 cactgtaact accgggaatg gcccctttag tttctctgga tgcgattaat acaccgtttt
      841 tgcgaacttc aaaaccataa cccgtgtatc ttgtacctcc cgggttatta ccacttcccg
      901 gatcgctata cgctattccg ttaaagataa tgggcggaat aatgatttga cggtcaaagt
      961 tatgatcatc gctgatggtg actgtaaccg tcccgtttgg tgtttccgta ttaccccacg
     1021 taccagcctg tttcgggaat gatttggata cagctttaac gaagtcacct ctgacctgag
     1081 tcgcctccag catgccctta atcgtacagt tttcatttac cgtgacattg ttgagcgtcc
     1141 cggcgttcgc attcactctg ccactgatat ccgcattttt agcggtcagc tttccgtctg
     1201 atgtcagaga aaataccgga ggactgccac cgctggtaat ggtgggggct gtcaggcgct
     1261 tcaggaacac gtcgttcatg aatatctggt taccctgcgc cacaaacatc ggcgtttcat
     1321 tcccgtttgc cgggtcaata aacgcgatac ggttagcggc aaccagaaac tggctcagct
     1381 tgccttcctc cgtgtcctcc atgctgaggc caatacccgc gacataatgt ttgccgtctt
     1441 tggtctgctc aattttgacg ccccacatgg cattccattt atcgttagcg tccttccact
     1501 ctttcgaaaa ctcatccagt ctgctggcgt tatcctccgt caaatcaact ttttccagca
     1561 gctctttacc gagatgggat tcggttatct tgcctttgaa aaaatccagg taaccttccg
     1621 catcatcgct cgcccgaccg acgccctcca cgaatgccga tttgccaacg gtgttcacac
     1681 tgcggatata aaagtaataa tcatggcccg gtttgatatt gatactggcg gctatccagt
     1741 acagcgccgt accaagatag cgggctgtgg tttcaacctg cctgatatcg gtaatccgcg
     1801 tttccgagaa ccagaactca aactgtaccg tcgggtcata aacggcaaga tgcggcgtgg
     1861 cggttatctg aaaatagccc ggcgtcagct caatcctcga cggtgctgcc ggtgcggcaa
     1921 tccggaacga taccgacgcc ggatcgccct gctgccccca cgcatttacc gcccggactg
     1981 tcagcctgta gttccccagc gccagttgcg tgaagcggta tgtggtttcc gtcgtccggg
     2041 ccgtgctgac cagccgctca ctgccgtcat ccgctgttac ggtcagacgg agcaggaagc
     2101 tcacgccctt caccaccttc ggcgtgtccc agcgcgccag cacctgatat tccccgctgt
     2161 ctgcggtgac ttcggcggtc aggtgctgca ccgctggcgg cgtgacacca ttcaccgtgc
     2221 cgctctggtc gccgtcaaag tgcgccccgt tatccacgat ggcttctttt tccggtacat
     2281 gctgcacggc agtgatggca tacgtgccgt catcgttatc acggatactc acacagcgga
     2341 acaggcgctg acgcaacgtc ggcagcttca gcccccacac actgtattct gcaacgccgt
     2401 caggaacccg gttcactttc accttaagtc cgtcggtgac ggactgaacc tccacgctga
     2461 ccggattacc acttccgtca accaggctta tcagcgtggt accggaggat ggcagcgtga
     2521 tttcacggtc gagcgtcagc gtccgggtct ggctgttcac cgccagcacg cgaccaccgg
     2581 tgctgatacc ggcatagtca tcatcgaaga tttcaataac atcgcccggt acatggcgaa
     2641 gcccttcagc acccacgctg aaatccacgg tctgcgtctc cagcagttcc gttttaatca
     2701 gccacagccc ggcgcggtgt gcctgccccc ggctggtaca gccaaaggca tccatcttcg
     2761 tgacattacg accgtaacgg gcaatggcct gcgtatcttc aacaagctct gtcgccgtct
     2821 cccagccgtt gttcgggtca atccagttca cctcaacggc attatggcgg tccttcaggg
     2881 cgctgaagct gtagcggaac ggcgcgccat catccggcat caccacatta ctgcggttat
     2941 aggtccacac cttatctgat ggtcggtcct gcacgaacgt cagcgtctgc ccgttccata
     3001 ccggcataca gcgcatcgcc gagcagaaat cactgagcac atcccacgcc ttgcgctgtg
     3061 tggtcaggta tgcattacag gtgatgcgcg gctccgtgcc gccaaagccg tccggtactg
     3121 actggtcgca gtactggccg atgacataca gcgcccattt gtccacatcc gccgcaccaa
     3181 gacgtttccc catgccgtag cgtggatggg tcagcatatc ccacagacac caggccatgt
     3241 tgttgctgta tgccggttta aacgttccgt cccagatacc gctgtattgt cgcgtctgcg
     3301 ggttatagtt cgacggcacc tgcagaatgc gcccgcgaag atgataatta cgactcacct
     3361 gctggctgcc gaactgctcc
//
LOCUS       DAZKGS010000281         3354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKGS010000281 DAZKGS010000000
VERSION     DAZKGS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..186)
                     /locus_tag="ACHOQY_004821"
     CDS             complement(<1..186)
                     /locus_tag="ACHOQY_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFP9680743.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRV"
     gene            490..1422
                     /gene="rfaD"
                     /locus_tag="ACHOQY_004822"
     CDS             490..1422
                     /gene="rfaD"
                     /locus_tag="ACHOQY_004822"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9680744.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1432..2478
                     /gene="rfaF"
                     /locus_tag="ACHOQY_004823"
     CDS             1432..2478
                     /gene="rfaF"
                     /locus_tag="ACHOQY_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9680745.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2482..>3354
                     /gene="rfaC"
                     /locus_tag="ACHOQY_004824"
     CDS             2482..>3354
                     /gene="rfaC"
                     /locus_tag="ACHOQY_004824"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9680746.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYG"
BASE COUNT          867 a          814 c          869 g          804 t
ORIGIN      
        1 aacccttggt ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag
       61 acgctcaaag taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt
      121 attagcagtc caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga
      181 tttcattact aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa
      241 tgtatactgt atattatcca ttcaaatgct tccgttattt atcatacatc ggaatattga
      301 tactaaagca ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca
      361 aactagtttg ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga
      421 tgtgatggta tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag
      481 gttacagtta tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaaa
      541 gccctgaatg ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc
      601 aagtttgtga acctggtgga tctgaatatc gcagactata tggataagga agacttcctg
      661 atccagatta tggctggcga agagttcggc gatgtcgaag cgattttcca cgaaggcgcg
      721 tgctcttcca ccaccgagtg ggacggcaag tatatgatgg ataacaacta tcaatactcc
      781 aaggagctgc tgcactactg cctggagcgt gaaatcccgt tcctgtacgc ttcttccgca
      841 gccacctacg gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg
      901 aacgtctacg gttactcaaa attcctgttt gatgaatatg ttcgccaaat cctgccggaa
      961 gcgaactcgc agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat
     1021 aaaggcagca tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca
     1081 ccgaagctgt tcgaaggtag cgagaacttt aaacgcgact tcgtctatgt tggcgatgtg
     1141 gcagatgtaa acctgtggtt cctggaaaat ggcgtttccg gcatcttcaa cctcggcacc
     1201 ggtcgtgcgg aatccttcca ggctgtagct gatgctacgc tggcttatca caagaaaggc
     1261 cagatcgaat acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag
     1321 gcagatctga caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa
     1381 ggtgtaacgg aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata
     1441 ctggtgatcg gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg
     1501 ctccaggcgc gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca
     1561 ttattatcgc ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg
     1621 ctggaaatcg gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc
     1681 gcctacgtct tacctaactc cttcaaatcc gcattagtgc ctttcttcgc gggtattcct
     1741 catcgcaccg gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc
     1801 gataaagaag cctggccgct aatggtggaa cgctatatag cgctggccta tgacaaaggc
     1861 attatgcgca cagcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc
     1921 gaaggtgaaa aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt
     1981 ggtttttgcc cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg
     2041 gagctggcaa agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctcggcgaaa
     2101 gatcatgaag caggcaatga gattcttgcc gctttaaata ccgagcagca ggcatggtgt
     2161 cggaacctgg cgggggaaac acagcttgat caagcggtta tcctgattgc agcctgtaaa
     2221 gccattgtca ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg
     2281 gttgccctgt atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc
     2341 gtgatccgtc tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat
     2401 caccagagct tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg
     2461 ttacaagagg aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat
     2521 gttctccata cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt
     2581 gactgggtgg tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga
     2641 gttattcctg tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct
     2701 gaacgcaaag cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct
     2761 caggggctgg taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga
     2821 ttggactggc aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat
     2881 attgcaaaac agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc
     2941 tatagcaaac cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg
     3001 cctacagatg ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac
     3061 tggccggaag aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt
     3121 aaacttccgt ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt
     3181 gcttatgttg aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg
     3241 gctaaatttg tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga
     3301 cccaatatca cggtttatgg accaaccgat ccgggattaa ttggtgggta tggg
//
LOCUS       DAZKGS010000282         3352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKGS010000282 DAZKGS010000000
VERSION     DAZKGS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..504
                     /locus_tag="ACHOQY_004825"
     CDS             <1..504
                     /locus_tag="ACHOQY_004825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680747.1"
                     /translation="LSFSEFKQALRIRMCSHNDAEHKYEKQNQTAENFVLEPGETLFK
                     IPVTCPICGFTSEELDDSCNNQETTKYVEDDTECARRTIISTSPNSRTNKSHYERVIN
                     PLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQRSANNCKRFIPVINFHVLKRIYWWL
                     RHCLMNP"
     gene            991..1179
                     /gene="dicB"
                     /locus_tag="ACHOQY_004826"
                     /gene_synonym="ftsT"
     CDS             991..1179
                     /gene="dicB"
                     /locus_tag="ACHOQY_004826"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9680748.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1176..1367
                     /gene="ydfD"
                     /locus_tag="ACHOQY_004827"
     CDS             1176..1367
                     /gene="ydfD"
                     /locus_tag="ACHOQY_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFP9680749.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            1461..>3352
                     /locus_tag="ACHOQY_004828"
     CDS             1461..>3352
                     /locus_tag="ACHOQY_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9680750.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGH"
BASE COUNT         1024 a          760 c          805 g          763 t
ORIGIN      
        1 ctcagcttca gtgagtttaa acaggcactc agaataagaa tgtgctctca caatgacgcg
       61 gagcacaaat atgagaagca aaatcagacc gcagaaaatt ttgttctgga acccggagaa
      121 acgcttttca aaattcccgt tacgtgcccc atttgcggtt ttacatcaga agaacttgac
      181 gactcctgta acaatcagga aacaaccaag tatgtcgaag atgataccga gtgcgcacga
      241 agaacgatta tatccacgag tccaaactcc aggaccaata aatctcacta tgagagggtg
      301 attaatccac tcccccaaac caataaaaaa gatgccggag gacaaaaaac ccaatggaac
      361 aacgggatat ctgtcaaaaa aagacgttcc attaaagaca aacaacgcag cgccaacaac
      421 tgtaagcgct ttataccagt aatcaatttt catgttctta agcggattta ttggtggttg
      481 cgacattgct taatgaatcc ttaaaactgt ggtgatttta aggataccac ctcgcctgac
      541 gtggttaaaa gcaggcacac aacacgaaag cgcacggcga agttcgtctc actgtacggt
      601 gtcgttaaat ttaattcgac cgtgcgcttc cggttgtggc aacccgcaaa atggcgcggc
      661 ggtaagtatg gcggggttat tccttccccg ttgaggacac cgggttgtca ggttgaccat
      721 acgcttaagt gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg
      781 cggtatcagt tttactccgt gactgctctg ccgccctttt taaagtgaat tttgtgatgc
      841 ggtgaatacg gctaagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
      901 tctctgtatc cgacgttaat tgttaactgg ttaacgtcac ctggaggcac caggcactgc
      961 atcacaaaat tcattgttga ggacgcgata atgaaaacgt tattaccaaa cgttaatacg
     1021 tctgaaggtt gttttgaaat tggtgtcact atcagtaacc ctgtatttac tgaagatgcc
     1081 attaacaaga gaaaacaaga acgggagcta ttaaataaaa tatgcattgt ttcaatgctg
     1141 gctcgtttac gtctgatgcc aaaaggatgt gcacaatgaa ttcagcattt gcgcttgttc
     1201 tgacagtttt tcttgtttcc ggagtgccag ttgatattgc agtcagtgtt cacaggacaa
     1261 tgcaggagtg tatgactgca gcaaccgaac agaaaattcc cggtaactgt tacccggtcg
     1321 ataaagttat tcaccaggat aatatcgaaa tcccggcagg tctttaaaac agttccgtaa
     1381 taaatatccg gtttcattct tatatgccag caatggcagg gatttgttca tccttaaatc
     1441 tgtcatgagg ttaaaacaaa atgagtaaag tctttatttg cgccgctatt cctgacgaac
     1501 tggcaacaag ggaagaaggc tctgtggctg tagccacagc cattgaagct ggcgacgaac
     1561 gccgtgctcg agcaaaattt cactggcagt tcctggaaca ttatccggct gctcaggact
     1621 gcgcttataa atttattgtc tgcgaggata aacctggcat accccgccct gccctcgatt
     1681 catgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcttttgtcc
     1741 cggttgagac tgaatccgat ccgatgaacg tcacttttga caagctggcc cctgaagtac
     1801 agaacgctgt catggttaag ttcgacacat gtgaaaacat caccgttgat atggttatta
     1861 gcgcacagga attgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt
     1921 tgatgaaaat gccagaagtt aacgccatgt atccggagct taagttgcac gccattgggt
     1981 gggttaagca taaatgtatt cctggtgcta aatggcccga aattcaggca gagatgcgca
     2041 tctggaaaaa acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg tctgttgttg
     2101 atctcgcccg cgccagagcc aatcaacagt acactgaaaa ttcaacagga aaaatcagcc
     2161 cggtcattgc tgccattcat cgcgaataca agcagacatg gaaaacactg gatgacgaac
     2221 tggcctacgc tctctggcct ggtgatgtgg atgccggaaa cattgacggc agcatccatc
     2281 gctgggcaaa aaaagaagtt atcgacaacg accgcgaaga ctggaagcgt atctcggcat
     2341 caatgcgcaa acagcctgat gcccttcgct acgaccgcca aactattttt ggccttgtcc
     2401 gtgagcgtcc gatcgacatt cacaaagatc ccgtagcact gaacaaatat atctgcgaat
     2461 acctgacgac aaagggcgtg tttgagaatg aagaaacaga cctgggcact gttgatgttc
     2521 tccagtcatc agaaacacaa actgatgcag tggaaactga ggtatctgat atcccaaaaa
     2581 atgaaaccgc gccggaagct gaaccatctg tagagcgtga ggggccgttc tatttcctct
     2641 tcgcagataa ggacggagaa aaatacggtc gcgcaaacaa actttctggt ctggataagg
     2701 cactggctgc tggcgccact gaaatcacaa aagaagaata ttttgcccga aaaaatggca
     2761 catacacggg cttaccgcaa aatgtagata ccgctgaaga ttcagaacaa ccagagccga
     2821 taaaagttac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg
     2881 acgccgataa gttgcttgct gcatcacgtg gtgaatttgt tgaagggatt agtgacccga
     2941 atgatccgaa atgggttaag gggatccaga cccgcgattc tgtgaaccag aaccagcatg
     3001 aatcggaacg gaactaccaa aaagcggaac aaaacagtcc aaatgcgtta caaaacgagc
     3061 cagaaacgaa acagcctgaa ccagtggcgc aacaggaagt ggaaaaagcc tgcaccgcct
     3121 gcggtcagac cggcggcggc aactgccctg attgtggcgc ggtgatgggc gacgcaacat
     3181 accaagaaac attcgatgaa gagtatcagg ttgaagttca ggaagatgat ccggaggaaa
     3241 tggaaggcgc tgaacatcca cacaaggaga acactggcgg caatcagcat cacaatagcg
     3301 ataatgaaac tggcgagacg gcagatcacc caattaaggt gaacggtcat ca
//
LOCUS       DAZKGS010000283         3290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKGS010000283 DAZKGS010000000
VERSION     DAZKGS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..443
                     /locus_tag="ACHOQY_004829"
     CDS             <1..443
                     /locus_tag="ACHOQY_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9680751.1"
                     /translation="AGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQG
                     PKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSHGWFPDTDGALITGLT
                     FLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            445..714
                     /locus_tag="ACHOQY_004830"
     CDS             445..714
                     /locus_tag="ACHOQY_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9680752.1"
                     /translation="MNILRKLMERLCGCGKHDDSENGELLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            826..1398
                     /locus_tag="ACHOQY_004831"
     CDS             826..1398
                     /locus_tag="ACHOQY_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9680753.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGRGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPENGVFIKNSGNSAVCTLFDSVSFSHLVREGGVHPL
                     TRETITPSMIVKPEDCIFDSSKGNFIIKDG"
     gene            1616..2314
                     /gene="nleD"
                     /locus_tag="ACHOQY_004832"
     CDS             1616..2314
                     /gene="nleD"
                     /locus_tag="ACHOQY_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFP9680754.1"
                     /translation="MRPTSLNLALHQSSTSSSMSDADIESLVKTSSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVIAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSQGFLQVRLHPLL"
     gene            3001..>3290
                     /locus_tag="ACHOQY_004833"
     CDS             3001..>3290
                     /locus_tag="ACHOQY_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9680755.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPS"
BASE COUNT          929 a          646 c          877 g          838 t
ORIGIN      
        1 atgctggtcc ggcaggcccg caggggccga aaggtgacag gggagagcgg ggagagaccg
       61 gtctgacggg aaatgcaggt ccacagggtc caaagggaga taccggtgcg gcaggcccgg
      121 caggcccaca gggaccgaaa ggagaaacag gtgcggctgg cccggtgggg gcaaccggac
      181 ctcagggacc gaagggcgac ccgggggaga cacaaatccg ttttcgtctg gggccggcga
      241 gcattattga gacaaacagc catggctggt tcccggatac agatggtgcg ctcatcaccg
      301 gactgacctt tcttgacccc aaagatgcca cacaggttca ggggctgttt cggcatttgc
      361 aggtcaggtt tggcgacggg ccgtggcagg atgttaaggg actggatgaa gtgggcagtg
      421 atacaggcag aacaggagaa tgacatgaat attttgagaa agcttatgga gcgtctgtgt
      481 ggttgcggaa agcatgatga cagtgaaaac ggggagttac ttacagcaca gctgcgactg
      541 ggaccagcag acattctgga gtccgatgag aatggcatta tcccggagca ggccagggta
      601 atcacgcagg tggtgatact ggatgcggat aaaaagcaga tacagtgcgt ggtaagaccg
      661 ctgcaaatcc tgcgtgctga cgggaggtgg gaaaatattg gcggaatgaa atagccgaca
      721 gcttcacaaa aaccggagtc cggctccggt ttttgttgtc atgtcatggt gatgtttgtt
      781 aataaagact tgagggaata tttatccgta tgaaggaata tggtaatgcc tggattaata
      841 tcatatgtgt catcggcttc attcgtgaat gagatgatgg agctgcgtca gcaggtaatg
      901 gaggggcaga ttggtggatt tctcctggga ggggagaggg ttagagtttc ttatatgcca
      961 gatacaggcc gttttttagc agaaagtgaa gggcggggac gggtttatgc agaattattg
     1021 aatattgctt ttaatgatgg agttaatgtg ctcagaaaca ggatattaag tgcgctccct
     1081 ggaatgggag ggcgaaactc tttgcaggaa aaaatatcgg agtgtgcctt tactgtcgat
     1141 attgaaaaac ttcaatgtcc tggtgatgcg cttcaatgtc caattacact ggagcagcct
     1201 gaaaatggtg ttttcattaa gaattcagga aattctgcgg tttgcaccct gtttgattcg
     1261 gtttcttttt ctcatttggt ccgtgaaggt ggggttcacc cactgacacg agagacaata
     1321 actccatcaa tgattgtaaa gccagaagat tgtatatttg acagttcaaa gggaaacttc
     1381 attataaaag atggttaaaa tgttttacat gaagcaatat tgattttcgg tagtttatat
     1441 aaaacaccac agacattcaa aatgtcagag gatgtcagtg gtggatgtac ctggagcgga
     1501 agttgtggaa gtaagttatg ttatatcgca ataaaccgaa tacatctgtg atgttacatt
     1561 ggtctgagct tcagtgtgtg gtgaatgaaa ctcgtaacat tttcagaggt ccttgatgcg
     1621 ccctacgtcc ctcaacttgg cattacatca gtcatcaacg tcgagttcaa tgtcagatgc
     1681 agatatcgag tctcttgtaa aaacatcgag cgttcaatgg ataaaaaaca atccgcaact
     1741 tcgtttccag gggactgatc ataatatata tcaacagatt gaagcagcgc tcgataagat
     1801 tggctctaca gagacagggc gtgtactcct gaatgctatt gaatcaatat cccgacttaa
     1861 atcagaaaca gtggtaatac acctcaattc ttccagacta ggagttattg cacatagaga
     1921 tatagatgct gagaaccatc ggggaactgg ttccgatttt cactgtaatc tgaatgcagt
     1981 tgaatatccc tgtggggagg ggattagcgt ggtggacttt catgcgacta ttgtttttca
     2041 tgagctgctc catgtgttcc acaatttaaa tggggagcgc ctgaaagttg agagttcccg
     2101 accagaatca caaaaatact ctccactttt actcgaagaa gccagaactg ttgggttggg
     2161 ggctttttca gaggaggtgc tttcagaaaa taaattccgc gaagagattg ggatgccccg
     2221 tagaacatcc taccctcacg actcagctct cattcatgat gacaatacag tgagtcaggg
     2281 attcctacag gtaagactgc atccattgct ttaggtgtgt ttcttgtggt gaatatatat
     2341 tggtgggata aaactgagat acataaggtc tcacaatggc ttgcaaggct ttgtgcttct
     2401 ctgtagaggc gacatgtttt tatccggtgc gccgtaaaac cccgtccttc agggcgtgga
     2461 ggatgtcaaa taataaggaa tagtggatat gtttgcagca aagcccgaga gcagtcaaag
     2521 aagtatttct ggattattca gtcctaggtc aaatggtgaa aacgcaagta ataaaccgtc
     2581 aagtggtatg gcgaacagaa tcaaaccagt tgtaagcgat tcacaaatcg gtagatatac
     2641 aactgacagt aatactctga aggatggatt gtgtagccaa gcccacatag gaactcaacc
     2701 ttatcggcca ggtgctgact cgccgctaac tgtgagatca ttcctacaaa ttacactttc
     2761 caacaataat tacaaatcat gagtctgccc tgtcccttct gccaggaagt gttacgcaac
     2821 gatatctcac caatctttcg caccactatt aatcaatgag ttagaggtga ttagtgacct
     2881 gagacagagc attagcgcaa ggtgattttt tgtccgtaag cgtaaaccga tcgccgtatg
     2941 tagccattag acaagaattg gtaacttaga cgcccatctg acacagacgg acatctaagt
     3001 atggaattac aggactggcg aaaagaacct cgtaaaaagt attcgaatga attcaaactt
     3061 cgtatggttg aactggcatc acaacctgga gcttgtgttg cacagattgc acgagaaaat
     3121 ggcgtcaatg ataatgttat tttcaaatgg ctcaggctct ggcagaacga agggcgtgtt
     3181 tcgcggcgtc ttccggtaac aacctcttct gacactggcg ttgaattatt acctgtagaa
     3241 ataacgccgg atgagcagaa agaacctgtg gcggccattg cgccgtcttt
//
LOCUS       DAZKGS010000284         3290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKGS010000284 DAZKGS010000000
VERSION     DAZKGS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..189
                     /locus_tag="ACHOQY_004834"
     CDS             <1..189
                     /locus_tag="ACHOQY_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9680756.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKTARGD"
     gene            194..538
                     /locus_tag="ACHOQY_004835"
     CDS             194..538
                     /locus_tag="ACHOQY_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP9680757.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            589..1122
                     /locus_tag="ACHOQY_004836"
     CDS             589..1122
                     /locus_tag="ACHOQY_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9680758.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1393..1959
                     /locus_tag="ACHOQY_004837"
     CDS             1393..1959
                     /locus_tag="ACHOQY_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9680759.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMIDFVLADTGKGKITKAD"
     gene            1959..2105
                     /locus_tag="ACHOQY_004838"
     CDS             1959..2105
                     /locus_tag="ACHOQY_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680760.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            2333..2539
                     /locus_tag="ACHOQY_004839"
     CDS             2333..2539
                     /locus_tag="ACHOQY_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9680761.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            2604..2828
                     /locus_tag="ACHOQY_004840"
     CDS             2604..2828
                     /locus_tag="ACHOQY_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680762.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3185..>3290
                     /locus_tag="ACHOQY_004841"
     CDS             3185..>3290
                     /locus_tag="ACHOQY_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680763.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCS"
BASE COUNT          852 a          704 c          898 g          836 t
ORIGIN      
        1 ggtgtgtcat acaccacgtc agcggtaggg acgggatact ggttactgca actgctggac
       61 aaagtctctc cgtcccagtg ggtggcaata ggtgtactgg ggagtctgct gtttggcctg
      121 ctgacgtatc tgactaacct gtatttcaaa atcagggagg accgccgtaa gacggcgcgg
      181 ggagactaaa gcgatgaaga aaaaatacga actggttgtt aaagagataa ataattaccc
      241 ggataagatt gctgttactg tggcacttga aattggcggg catccgtcgt tgttgttgcc
      301 acatgtggcg attagtcttg accgtactga aggtgccacg ctggagtttt acgaagctga
      361 ggcgaaaaag caggcgaagc agtttttcat ggatgttgct gccgggttat gtgaagggga
      421 tggtccgtta ccggaaaagc gtcccgtaat tttagaggcg caggatgtgt tgataaccta
      481 cagaggaaaa ctaccgggaa taattacggg ttctctgaag actccaccgc tggcctgaag
      541 acttaacata tccagggatt tgaaatcgat aaaccctgat aaatatccat gaacgcaaaa
      601 atcagatacg gcctgtcggc tgccgttctg gcgctgattg ccgcaggtgc gcctgcgcct
      661 gaaatcctcg accagtttct ggatgaaaag gaaggtaacc acaccacagc ataccgtgat
      721 ggtgcgggta tctggaccat ctgccgtgga gccacccggg tggatggtaa gcctgttatt
      781 cctggcatga agctgtcgaa ggaaaaatgc gaccgggtta acgccattga gcgtgataag
      841 gcgctggcat gggtggagaa aaacatcaga gtgccactga ccgaacccca gaaagcgggg
      901 attgcgtcat tctgtccgta caacattggc cccggtaagt gtttcccgtc gacgttttac
      961 agacggatta atgcaggaga tcgaaaaggt gcctgcgaag ctattcgctg gtggattaag
     1021 gacggtggca gggactgccg tattcgctca aataactgtt acggtcaggt atcccgtcgt
     1081 gaccaggaga gcgcgctggc gtgctgggga atcgacagat aagcagaata ttttgctgaa
     1141 aaatgaggtt tgcttacatg gatggataac acgaaatcct gcaaattggc aaaatgtaag
     1201 tgaataaagt caaaacagtt gtttaacact caggcaccgt aatgatgcct ttgtcatttc
     1261 tgcgcatctc acgcgcatct cacaacacag aacctttcag gatgaccctt gaggataccg
     1321 gtttggctgt cggtgccttt ctgtgggctg gattcctgtg agacaaggtt catcactaaa
     1381 aggaaataac cgatgaatat gatggccgtg ccgtttcacg gcaactctct ttatgtagtt
     1441 aaccataatg gcgaaccata cgttcccatg aaacctgtcg ttgcggggat ggggctggcc
     1501 tggcaatcac agttggctaa gttaagacag cgttttgcgt caactataac ggaaatcgtt
     1561 atggttgctg aggatgggaa acaacgcaat atggtgtcca tgccacttcg aaaacttgcc
     1621 ggctggctac aaaccattaa tcccaacaaa gtaaaacccg aaatccgcga taaggtcatc
     1681 cggtatcagg aagagtgcga cgatgttctt tacgagtact ggacgaaggg ttttgtcgtt
     1741 aatccccgta aaatgagcgt gatggaagaa ctcaaccagg cttgtgctga catgaaacgg
     1801 gataaaaaca ttgccagtgt gtttgctacc gggctgaatg agtggaaaca ggttaaagcc
     1861 gcgcatgtat caaaaatccg tacgctggta aatgaagcga atatgattga ttttgtcctg
     1921 gctgatacag gcaaagggaa aataacaaag gcggattgat ggggtggcta atgatatcag
     1981 ataaactcat aacgctggtg aagagcctct gtgtacttgt cggcatttca tttttagtca
     2041 tgctggttgc cattttcttt tccaccgcct ggcgagtcct gacgttatcg ggactggtgg
     2101 ggtgaaagag agatgaaccg tgttctgtgt gtggtgatta ttgtcatggc ggttggctgt
     2161 ggtgcgctgt ggctggcaac aaaccattac cgtgacaacg cgctcaccta caaagcgcag
     2221 cgcgataaaa aagccagaga gctggaacag gcgaatgcca ccattactga catgcaggtg
     2281 cgccagcgtg atgttgctgc gctcgatgca aaatactcga gggaattagc cgatgcgaga
     2341 gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc gtaagcgcct gcggatcaac
     2401 gccacctgcc ccggtaccgt gcgtgaagcc accggcacct ccggcgtggg caatgatgcc
     2461 gccgtcgaac tctctccggt tgctggacga aacgttctcg gtatcagaga cggaatcatc
     2521 agcgaccagg cagcattgag aatgcttcag gaatatatcc gcactcagtg tattaactag
     2581 tatttttgtt attcggagaa tgcatgaaga aattattggt aaccgtaaag ccttttcagg
     2641 gaacaattcc gttccgtatt ttgcagcgtg gtcgtgttct tgttgaaggt tcgttcagtg
     2701 gtaaatgtac gcaattacac tccaggacct ttcaggtgaa tgccacgaat gaagagctaa
     2761 ccgttgagtg tacgatgaat gccgctaaat gccgcatggt atccgctgca ttacagccag
     2821 tgtgttgagc gaccttatta ttcatgcgcg gtattgtcgc cgtattcctg cattaacaga
     2881 gaccgcagcc cgaccgggag aatcctctgc gcgagtgtgc ggggataatc aaaaacgata
     2941 cacaccgggg tttaccgcgt taacggagcg cggcgttgtc ccctcatggt cgctggtccg
     3001 gtgcgatggt ggaagaagcc ggatgtttat cactattaat tgataacaca gaaatggatt
     3061 cattgatttt cagcacgttt ttgtattcgt attattgaac atctgtttat tttactttta
     3121 acatattgat aataaaaaga gctgtaaatc tttagatgag tcgattttgt ccggggaagt
     3181 tcaaatggat tttatgctga cggtttctgg tgtggttatc ctgtccattg cttatactgc
     3241 agataaatat ggctgccatt tgttatcacg tattggcgct tattgctcgt
//
LOCUS       DAZKGS010000285         3271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKGS010000285 DAZKGS010000000
VERSION     DAZKGS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..603
                     /gene="yibB"
                     /locus_tag="ACHOQY_004842"
     CDS             <1..603
                     /gene="yibB"
                     /locus_tag="ACHOQY_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP9680764.1"
                     /translation="RSRIKKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVN
                     KAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTSQQQ
                     VFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKR
                     PDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            878..2074
                     /gene="kbl"
                     /locus_tag="ACHOQY_004843"
     CDS             878..2074
                     /gene="kbl"
                     /locus_tag="ACHOQY_004843"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9680765.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2084..3109
                     /gene="tdh"
                     /locus_tag="ACHOQY_004844"
     CDS             2084..3109
                     /gene="tdh"
                     /locus_tag="ACHOQY_004844"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9680766.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
BASE COUNT          812 a          722 c          863 g          874 t
ORIGIN      
        1 agaagtcgta tcaaaaaaat tcaaaaagat gagtcgttta caaatagact tgagcctcga
       61 caattaaaaa acccagagta ctggtcacca gagtatgttt tggtatgtaa ccttaaggcc
      121 tattttgtaa ataaagctat caacatgggc ttagtgaaaa cgcctcttgt tgcgtggata
      181 gattttggct attgccgtaa gccaaatgta actcgaggat tgaaaatttg ggatttccca
      241 tttgatgaaa gtaagatgca tcttttcact ataaaaaaag ggcttactgt cacatctcaa
      301 caacaggttt ttgatttcat gattggcaat catgtatata ttattggggg cgcaattgtt
      361 ggttcacagc ataaatggaa agaattctat aaattagttc tggaaagtca aaaaataaca
      421 ctaaataata acattgtgga tgatgaccag ggcatttttg tcatgtgcta ttacaaacgc
      481 cccgatctct tcaacctaaa ctatctgggg agaggaaaat ggttcgattt gtttcgctgc
      541 ttcaggagta acactttagg ggcaaaaatg caggcgctga gaattttcct ttcaagaaaa
      601 taggtttccc tccattagtg ggtagcgttt tgataccaac tgagatagga aaaatgatgc
      661 tttggcatca ttttttcttt ttatatcaat gcagtaaaat caatcatcat tatgcccagc
      721 caaaactgta cagtttttct actatctgta ggtgttgaga gacatcaagt tacacgttat
      781 ttatcctgaa ttttgcagaa gtgttaacgc gtcatctcgt cgcgacctat aagtttgggt
      841 aatatgtgct ggaatttgcc ctgtctggag aatcgcaatg cgtggagaat tttatcagca
      901 gttaaccaac gatctggaaa ccgcacgggc ggaagggttg tttaaagaag agcgcattat
      961 tacgtctgcg cagcaagcag atatcactgt ggctgatgga agccacgtca ttaacttttg
     1021 tgccaacaac tatctcgggc tggcgaatca tcctgatctg attgcggcgg caaaggcggg
     1081 aatggattct cacggtttcg gcatggcttc ggtgcgtttt atttgcggca ctcaggacag
     1141 ccataaagag cttgaacaaa aactggcggc cttcctgggg atggaagatg cgattctcta
     1201 ctcttcctgc tttgatgcta acggtggcct gtttgaaacg ctgctgggcg cggaagacgc
     1261 cattatctcc gacgcactga accacgcgtc tattattgat ggtgtgcgtc tgtgtaaagc
     1321 taaacgctat cgctatgcca acaacgatat gcaggagctt gaagctcgtc tgaaagaagc
     1381 gcgtgaagcc ggtgcgcgtc atgtgctgat tgccaccgat ggtgtattct cgatggacgg
     1441 cgtgattgcc aatctgaagg gcgtttgcga tctggcagat aaatacgatg ccctggtgat
     1501 ggtagacgac tcccacgcgg tcggttttgt cggtgaaaat ggtcgtggtt cccatgaata
     1561 ctgcgatgtg atgggccggg tcgatattat caccggtacg cttggtaaag cgctgggcgg
     1621 ggcttctggt ggttataccg cggcgcgcaa agaggtggtt gagtggctgc gccagcgttc
     1681 tcgtccgtac ctgttctcca actcgctggc accggccatt gttgccgcgt ccatcaaagt
     1741 actggagatg gtcgaagcgg gcagcgaact gcgtgaccgt ctgtgggcga acgcgcgcca
     1801 gttccgtgag caaatgtcgg cggcgggctt taccctggcg ggagccgatc atgccattat
     1861 tccggtcatg cttggcgatg cagtagtggc gcagaaattt gcccgtgagc tgcaaaaaga
     1921 gggcatttac gtcaccggtt tcttctatcc ggtcgttccg aaaggtcagg cgcgtattcg
     1981 tacccagatg tctgcggcgc atacccctga gcaaattacg cgtgcagtag aagcattcac
     2041 gcgtattggt aaacaactgg gcgttatcgc ctgaggatgt gagatgaaag cgttatccaa
     2101 actgaaagcg gaagagggca tctggatgac cgacgttcct gtaccggaac tcgggcataa
     2161 cgatctgctg attaaaatcc gtaaaacagc catctgcggg actgacgttc acatctataa
     2221 ctgggatgag tggtcgcaaa aaaccatccc ggtgccgatg gtcgtaggcc atgaatatgt
     2281 cggtgaagtg gtaggtattg gtcaggaagt gaaaggcttc aaaatcggcg atcgcgtttc
     2341 tggcgaaggc catatcacct gcggtcactg tcgcaactgt cgtggcggtc gtacccatct
     2401 gtgccgcaac acgatcggcg tcggcgttaa ccgtccgggc tgcttcgccg aatatctggt
     2461 gatcccggcg ttcaacgcct tcaaaatccc agacaatatc tccgacgacc tggcttccat
     2521 ttttgatccc ttcggtaacg ccgtgcatac cgcgctgtcg ttcgatctgg tgggcgaaga
     2581 tgtgctggtt tctggtgcag gtccgatagg tattatggct gcggcggtgg cgaaacacgt
     2641 tggtgcacgc aatgtggtga tcaccgatgt taacgaatac cgccttgagc tggcgcgcaa
     2701 aatgggtatc acccgtgcgg ttaacgtcgc gaaagaaaac cttaatgatg tgatggctga
     2761 actgggcatg accgaaggct ttgatgtcgg tctggaaatg tccggcgcgc cgccagcgtt
     2821 tcgtaccatg ctcgatacca tgaaccacgg cggtcgtatt gcgatgctgg gtattccacc
     2881 gtccgatatg tctatcgact ggaccaaagt aatctttaaa ggcttgttca ttaaaggtat
     2941 ttacggtcgt gagatgtttg aaacctggta caagatggcg gcgctgattc agtcaggtct
     3001 ggatctctcg ccgatcatta cccatcgttt ctctatcgat gatttccaga agggctttga
     3061 cgctatgcgt tcgggccagt ccgggaaagt aattctgagt tgggattaac acgaacaagg
     3121 gctggtattc cagccctttt gcctgaggat aatccgttaa atatgtaaaa tcctgtcagt
     3181 gtaataaaga tatcgtaatt gcgctgatct cttatatagc tgctctcatt atctctctac
     3241 cctgaagtga ctctctcacc tgtaaaaata a
//
LOCUS       DAZKGS010000286         3269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKGS010000286 DAZKGS010000000
VERSION     DAZKGS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(541..726)
                     /locus_tag="ACHOQY_004845"
     CDS             complement(541..726)
                     /locus_tag="ACHOQY_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9680767.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
     gene            complement(954..1103)
                     /locus_tag="ACHOQY_004846"
     CDS             complement(954..1103)
                     /locus_tag="ACHOQY_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680768.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            complement(1103..1672)
                     /locus_tag="ACHOQY_004847"
     CDS             complement(1103..1672)
                     /locus_tag="ACHOQY_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9680769.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            complement(1947..2480)
                     /locus_tag="ACHOQY_004848"
     CDS             complement(1947..2480)
                     /locus_tag="ACHOQY_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9680770.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(2485..2700)
                     /locus_tag="ACHOQY_004849"
     CDS             complement(2485..2700)
                     /locus_tag="ACHOQY_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9680771.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2778..3023)
                     /locus_tag="ACHOQY_004850"
     CDS             complement(2778..3023)
                     /locus_tag="ACHOQY_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9680772.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            complement(3064..3243)
                     /locus_tag="ACHOQY_004851"
     CDS             complement(3064..3243)
                     /locus_tag="ACHOQY_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9680773.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
BASE COUNT          834 a          906 c          678 g          851 t
ORIGIN      
        1 gtgatcatgt agcataagta acctccgttt atctacagac tgccatcctg aatttaccgt
       61 ccttaataag acaacaataa aaaactaatt atataaaaac aataaaaata atatacaaaa
      121 aaactaaaca acaaagaaaa gcaaatcata tctggcattt taattgaaag agcaattacc
      181 gaacaaaaga cgctgactat atactcaaaa ccaaacaaca attctgccaa tcaggtatca
      241 tggcaacaca cggaattacc atgtttttgc cttctctgcc catacaatac gggcatatac
      301 ttcatactct attgtaatat ttctatccat gcgccccact ccatttacct gtaaataata
      361 ttcaaaatat ttatcacaga aatcgttttt ggccatgaac tgagcacact ataaagtccg
      421 gaactgactc tttgttaaat taccttaacg ttaccagtaa caccttcgta acaaaacatc
      481 acggtataca ctgggtacgg atatattcct gtgctccttc cagttgcttc tgcattgcca
      541 tcagccgttc tctgaggatg aaataatccc gttcagcggc ttctgccagt cggggaccgg
      601 ttgcattatc cacgccggag gtgatggggg ctttacgcaa ggagcctgga cagttggcgt
      661 tgatgcgcag gcgcttacga ccagcggcaa catcagcacg cagagtttca ttttcagctc
      721 tcgcatcggc taattccctc gagtatctgg catcaagtgc agcaacatca cgctggcgct
      781 gctgcatatc agtaatggtt gcatttgcca gctccagctc actgactttt ttatcgcgct
      841 gctctttgta ggttatggcg ttatcgcggt aatgattcag ccccagacta agtgcaccac
      901 aggctaccag caggacaata atcaccacac acagaacacg gttcatctct ctttcacccc
      961 accagtcccg ataacgtcag gactcgccaa gcggtggaaa agaaaatggc aaccagaatg
     1021 actaaaaatg aaatgccgac gattacacag aggatcttcg ccagcgttat gagtttatcc
     1081 gatatcatta gccaccactc catcaatccg cctttgttat tttccctttg tctgtatcag
     1141 ccaggacaaa atcaatcagc agattcgctt cgtttatcaa tgtgcggatt tttgatacat
     1201 gcgcggattt aacctgtttc cactcattca gcccggtagc aaacacactg gcaatgtttt
     1261 tatcccgttt catgtcagcg caagcctgat tgagttcttc catcacactc attcgacggg
     1321 gattaacgac aaaacccttc gtccagtatt catagagaac atcgtcacac tcttcctgat
     1381 actggattac cttgccgcgg atttcgggtt ttactttgtt gggattgatg gtttgtaacc
     1441 agcctgcaag ttttcgaagc ggcagggaca ccatattgcg tcgtttccca tcctcagcaa
     1501 ccataacgat ttccgttata gttgacgcaa aacgctgtct taacttagcc aactgtgatt
     1561 gccaggccag ccccatcccc gcaacgacag gtttcatggg aacgtatggt tcgccgttat
     1621 ggttaaccac ataaagagaa tcgccgtgaa acggcacggt catcatattc atcggttatt
     1681 tccttttagt gatgaaccct gcgcacagga ataaccagcc caaagagggt taaccagacc
     1741 actgccggtt atccaccagg gctcatcctg aaaggttctt tggtttattt acgcttgtgc
     1801 gaagcgcaga aatgacaaag gcaccattac ggtgcctctt catgaaacaa tcttgttgac
     1861 tttattcact tacattttgc cagttcgcag gatttcgtgt tgtctacccg cgttggccaa
     1921 cgtcattttt cagcaaaata ttctgcttac ctgtcgatac cccagcatgc cagcgcactc
     1981 tcctggtcac gtcttgatac ctgaccgtaa cagttgtttg aacggatacg gcagtctctg
     2041 ccaccgtcct taatccacca gcgaatcgcc tcacacgctc ccctgcgatc gcctgcatta
     2101 agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat
     2161 gacgcgatac ctgctttttg tggctcggtc agtggaactt ttatattacg ctccacccat
     2221 gccagcgcct tatcccgttc gatagcgtta acccggtcgc atttttcctt cgacagcttc
     2281 atgccaggaa tcacaggctt accatccacc atgatggcgc ctcggcagat ggtccagata
     2341 cccgcaccat cacggtatgc cgtggtgtgg ttgccttcct tttcatccag aaactggtcg
     2401 aggatttcag gcgcagaagc acctgcggca atcagcgcca gaacggcagc cgataaacca
     2461 tagcggagtt tcctgctcat cagcttactc tccccgtgcc gccttacgcc tgtcctctct
     2521 gattttgaaa tacaggttcg tcaggtacgt cagcagacca aacagcagac tccccagcac
     2581 gcctattgcc gcccactgag acggggaaac cctgtccagc aactgcagga accagtagcc
     2641 cgttcccacc gctgacgtgg tgtatgacac acctgttgtg attttttcca tctggttcat
     2701 accccgcctc ccgcaatccg gaagctcaca acaataaaaa agaccaccgg cacacaccga
     2761 tggtccctga cgcatgctta catcatcatg tcgctgtccg gtgtggggtc accgctatct
     2821 gaagcactcc cctcacccgc gatgccttcc ggctccggag ctgccggtgc gcccagcagt
     2881 tcatccagaa tggcatccac ttctgcatca agacgcgctt ccaggttatg gcgaagtttc
     2941 tgtttcagtg cgctccggac ttcttcagag cgcaggactt ccttcactgc ttcagcagtg
     3001 accagggatg taatttctga catgggattt tctcgtcgaa aggtgtgatt aagaaagttg
     3061 ccgctaaatg agcggctctt cgggtttgct tccggctgac tgaccggcgc tgattttctc
     3121 agcggccctt ttgtcaatct gcctgcgcca gcaatcgcgc actgccctgt acccacccga
     3181 aaggagatac agcacacaga ccaccgtaca gaagtacagc attaactggt tcagaaatgt
     3241 cataatttct ttccgttatt gttgacaat
//
LOCUS       DAZKGS010000287         3236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKGS010000287 DAZKGS010000000
VERSION     DAZKGS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..851
                     /locus_tag="ACHOQY_004852"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHOQY_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHOQY_004853"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHOQY_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHOQY_004854"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHOQY_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          791 a          860 c          767 g          818 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgcag ccccccctcc ctgctgtata ccccagtacc acaacg
//
LOCUS       DAZKGS010000288         3206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKGS010000288 DAZKGS010000000
VERSION     DAZKGS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHOQY_004855"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHOQY_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9680774.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHOQY_004856"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHOQY_004856"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9680775.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHOQY_004857"
     CDS             1252..1521
                     /locus_tag="ACHOQY_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9680776.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHOQY_004858"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHOQY_004858"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9680777.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3206
                     /gene="dsbA"
                     /locus_tag="ACHOQY_004859"
     CDS             2601..>3206
                     /gene="dsbA"
                     /locus_tag="ACHOQY_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9680778.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVK"
BASE COUNT          804 a          774 c          785 g          843 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattga tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatggcgatt
     1021 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcgca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtc aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaa
//
LOCUS       DAZKGS010000289         3195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKGS010000289 DAZKGS010000000
VERSION     DAZKGS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..70)
                     /locus_tag="ACHOQY_004860"
     CDS             complement(<1..70)
                     /locus_tag="ACHOQY_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9680779.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRR"
     gene            complement(177..521)
                     /locus_tag="ACHOQY_004861"
     CDS             complement(177..521)
                     /locus_tag="ACHOQY_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680780.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(550..717)
                     /locus_tag="ACHOQY_004862"
     CDS             complement(550..717)
                     /locus_tag="ACHOQY_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680781.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(790..1074)
                     /locus_tag="ACHOQY_004863"
     CDS             complement(790..1074)
                     /locus_tag="ACHOQY_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9680782.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(1067..1210)
                     /locus_tag="ACHOQY_004864"
     CDS             complement(1067..1210)
                     /locus_tag="ACHOQY_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680783.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1220..1369)
                     /locus_tag="ACHOQY_004865"
                     /pseudo
     CDS             complement(1220..1369)
                     /locus_tag="ACHOQY_004865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
     gene            complement(1846..1983)
                     /locus_tag="ACHOQY_004866"
                     /pseudo
     CDS             complement(1846..1983)
                     /locus_tag="ACHOQY_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1985..2542)
                     /locus_tag="ACHOQY_004867"
     CDS             complement(1985..2542)
                     /locus_tag="ACHOQY_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9680784.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            complement(2539..3096)
                     /locus_tag="ACHOQY_004868"
     CDS             complement(2539..3096)
                     /locus_tag="ACHOQY_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680785.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            complement(3093..>3195)
                     /locus_tag="ACHOQY_004869"
     CDS             complement(3093..>3195)
                     /locus_tag="ACHOQY_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFP9680786.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
BASE COUNT          759 a          814 c          677 g          945 t
ORIGIN      
        1 atcgacgaac tgtttcaagg cttcttgggc gtcgctggcg tgcattccac tcctgaagtg
       61 tcaagtacat cgcaaagtct ccgcaattac acgcaagaaa agccgcattg atgcggcgat
      121 ggtaggtctg gatatcattg agccatgaac aggcctcatc gagtgtgagg ctgtggttag
      181 tcctttcgta actcgctgat tctcctgtaa gtctctggtg ctttgtttcc gtgtatcttc
      241 atttcagact tcaacagagc aacgagggaa tcccattcgt tgaggatgcc tttgaatgcc
      301 ggaacgcgct ttgcaacctt gtcgaatgaa tctctgattt ctggaatctt ctcaacaagt
      361 gcaacgcatc gtcggaagtc tgctgcgtca tggggagcgc cgaagtgatg accatagata
      421 ttctttttca ggccacatgc gattgaggca agagttgcgc tactgatgcc aacatcgcca
      481 gtcgattgcc atttcaaaac cttcatagac aaatctgaca tttcttgtct ccaataaaaa
      541 aaacctccat caggcggctt ggtgttcttt cagttcttca attcgaatat tggttacgtc
      601 tgcatgtgct atctgcgccc atatcatcca gtggtcatag cagtcattga tgttctctgc
      661 ttcgataact ctgttgaatg gttctccatt ccattcacct gtgactcgga agtgcattta
      721 tcatctccat aaaacaaaat ccgccgtagc gagttcagat aaaagaaatc cccgcgagtg
      781 cgaggattgt tattcattgc cgatattcac ctttatcgcg aacaccttta ccggtttatc
      841 accgaagtgc ggatgtgtga ttgtcttgat ttcatatccg tcatacggaa catcaattct
      901 gcggctggaa tcgtcgcgct tcggatatcc ctttgtgata atcaggcggt catactcccg
      961 gaacataatt cgcttattcc aatagtcatt acacaggcga tactcttccg ttttctctcc
     1021 tcgaatcatg gcatcgaagt attcaccttt gacggcaagt tgtaggttag ccacggttaa
     1081 cctcctgcgg agtagccttt acaagcactg gtgcaaatcc atcttcatta aggttatgaa
     1141 tatagacttc tgttctcctg cgctcttcaa cgtttaatat tgtttctgga tcataaaccc
     1201 atactttcat tcgactattc catgaagaaa tcgcttcaga tctagttcgt ttttctggtc
     1261 cttgggcacc acatttgcat gacacatagc gcattttccc tctgatactg aatgagtatc
     1321 cgatgttaag cacagtggaa ccacagaatg ggcagcgata tagattcata aatcatccac
     1381 cttaggcgca gctgagacag caacattcca gaactcagaa aacatattgt atgcaccaga
     1441 tagattggaa gtagcatacc ctccaagctc acttctaatc tgaacggctc tcatcatttc
     1501 cggggttaac tccttcggca ccataaccca accatccgga gttaccggag agttgccacc
     1561 gggaatattt tccggaatat tttgttgtgc gttttgtggt tgttctgatt taccctgaag
     1621 catggcggcg cggcaggcgt tccagccagc tgttcgccca agcgcgtaaa cttcagatgg
     1681 atcaagataa tcaatgtcat gcccgtcctc atcgtcgttc tcaggtaatg cagcaggtac
     1741 taccggtact ggcggagcgg cgtagacctc aataattcca ttatcaatag gccattctcc
     1801 atccttgagg tagtcacttg tgccgtcaac ttgctgttct gcaatgtgga atgcacctat
     1861 tggttttgct tccagcgatg ccagagcaat ccgtgccagt tccatttgtt cgccactagt
     1921 aagtccgtta tcaagcgggg atttaatgaa caattcgata cgttctttag tgatagtgct
     1981 catatcactc tcctttgatg cgaatgccag ctgcgcttgc tgattcttca tatgcgcgtt
     2041 tagcagcgtt aaggattgct gccagtggcg tatagccgcc atccattcgg attgtgttgt
     2101 ggatgccagc cattgtgtcg cgcaatttgc tgtggctagc agacagttct gctatgcact
     2161 tctctgcggc atcggctcga tctttttcac gctggaacat tttgtgagtg cggtctatag
     2221 cttcgattcg tgccgctgat gtcttctgca cggcttcaag ctcaacacgc agcttcccaa
     2281 ccgtaagagc aatatcctcg ttcttctggt cgcggcgttt gatgtattgc tggtttcttt
     2341 cccgttcatc cagtagtgcc agcacggttt ctggcccggt cagaaatttg aaggcgttga
     2401 gcgcatcaat atccacaccg taatccttaa gttcctgttc agttaacaaa tcatcatcaa
     2461 ctggcaacat taacaggcgt tccattgccg gaattgcacg ctctgccgcc tcacgcagtg
     2521 cctgatagtc aatcttgctc attggatgac tcctttacga agctgttcgg cgatatcttc
     2581 gagaacgcca tcagagaatg aacggtcaaa atcgccttcc ggcgcattgg ccataaactc
     2641 agtagaggta agaatcatcc gggcaatatc agcggcgttc ttcgcagtat catcaataaa
     2701 accagcttcc caggcagcca gcattctgtt cgccacaaag taagcgccct ccttgcgtgc
     2761 ttcagtcttc acttcagcca gaaaagcatc ggtggctggg gtttcagtaa tatcatcttc
     2821 ccattcgcta aactcctcac gacaaaagtc attaaattcc ttctcagatt gcttaagcga
     2881 ggaattttca gcagccatct tcgcgcattt agcctcaagg ttatcaatcg tgattccagc
     2941 agaacgacac tcccgcaacg ccgtttctag ttttgattca agttcaccga acttacggac
     3001 aagatattca tcgtttgttt cgtcaacctt taaatcactt gggatgcatt tacctttcag
     3061 aaaaccatcc atctcaatta gtgtcatttg tttcatttct tcccactccg ccacatcgca
     3121 ttcagatatt tgttgtcatt aacagaaccg aaactctttc tcttaagcaa ttcctctctc
     3181 gatggcattg gcttt
//
LOCUS       DAZKGS010000290         3162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKGS010000290 DAZKGS010000000
VERSION     DAZKGS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1581
                     /locus_tag="ACHOQY_004870"
     CDS             <1..1581
                     /locus_tag="ACHOQY_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9680787.1"
                     /translation="PDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFE
                     DDEGTNQGTANTLSSPVPETDAVETAIQDNEKTECKVEVESSVEREGPFYFLFSDKDG
                     EKYGRANKLSGLNKALAAGATEITKEEYFARKNGTYSGSQQNTGASDTIAQPEPVKVT
                     ADEVNKIMQAANISQPDADKLLAVSRGEFVAGISDPNDPKWVKEIETRDSVNQNQQET
                     EQNDQKAEQNSPNALQNEPETKQSEPVVQQEPEKVCTACGQSGGGNCPDCGAVMDNAT
                     YQETFDEENQVEAQENDPEEMEGAEHPHKENTGGNQHHASDSETGEAADPLIKVNGHH
                     NLTSTSRAGIHLMIDIETMGKNPNAAIISIGAIFFDPQTGDMGPEFSKTIDLDTAGGV
                     IDRDVIKWWLKQSREAQSAILTDEIPLDDALLQLREFIAENSGEFFVQVWGNGANFDT
                     VILRRSYERQGIPCPWHYHNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQ
                     AKYVSAIWQKLIPSQADF"
     gene            1651..1902
                     /gene="xisR"
                     /locus_tag="ACHOQY_004871"
     CDS             1651..1902
                     /gene="xisR"
                     /locus_tag="ACHOQY_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9680788.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDCMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            1922..>3162
                     /locus_tag="ACHOQY_004872"
     CDS             1922..>3162
                     /locus_tag="ACHOQY_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFP9680789.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSDNNNAQVTLLNT"
BASE COUNT         1013 a          706 c          764 g          679 t
ORIGIN      
        1 cctgatgcgc ttcgctacag ccgccagact atttttggcc ttgtccgtga acgcccgatc
       61 gacattcaca aagatcccgt ggcactgaac aaatacatca ctgaatacct gactacaaag
      121 ggcgtgtttg aagatgacga aggaacaaat cagggcacag ctaatactct ctcgtcgcca
      181 gtaccagaaa ctgacgcagt ggaaacggca attcaggaca acgaaaaaac cgaatgcaaa
      241 gtggaagtcg aatcatctgt agagcgtgag gggccgttct acttcctctt cagcgacaag
      301 gatggcgaaa aatacggtcg tgcaaacaaa ctttctggtc tgaataaggc gctggctgca
      361 ggggctactg aaatcacgaa agaagaatat tttgcccgca aaaacggtac atactcaggt
      421 tcacaacaaa atactggtgc atctgacacg atcgcacaac cggagccggt aaaagttacc
      481 gctgacgaag taaacaaaat tatgcaggca gccaatatca gccagcctga cgccgataag
      541 ttgcttgctg tatcacgtgg tgaatttgtt gcagggatta gcgacccgaa tgatccgaaa
      601 tgggtgaagg agattgaaac ccgcgattca gtgaatcaga accagcaaga aacggaacag
      661 aacgaccaga aagcggaaca aaacagccca aatgcgttac aaaacgagcc agaaacgaaa
      721 caatccgaac cagtagtgca acaggaaccg gaaaaagtct gcaccgcctg cggtcagagc
      781 ggtggcggca actgccccga ttgtggcgct gtcatggaca acgcaacata ccaggaaaca
      841 ttcgatgaag agaatcaggt tgaagctcag gagaatgatc cggaggaaat ggaaggcgct
      901 gaacatccac acaaggagaa cactggcggc aatcagcatc acgccagcga tagtgaaact
      961 ggcgaggcgg cagatccctt aattaaggtg aatggtcatc acaatctcac atccaccagc
     1021 agagcgggga ttcatctgat gatcgacatt gaaaccatgg gaaaaaatcc caatgccgcg
     1081 attatctcaa taggcgcaat atttttcgat ccacaaaccg gagatatggg accggaattt
     1141 agcaagacca tcgatctgga tactgctggc ggagttattg atcgtgacgt cattaaatgg
     1201 tggctgaagc aatcacgtga agcgcagtct gccattctga ccgatgaaat cccgttagat
     1261 gatgcactac tgcaattgcg ggaatttatc gccgaaaact ccggtgaatt ttttgttcag
     1321 gtctggggaa atggagccaa cttcgacacc gtgattttac gccgttcata cgaacggcag
     1381 gggatcccct gcccctggca ctaccacaac gatcgcgatg tacgcacaat cgttgagcta
     1441 gggaaagcca tagacttcga tgccagaaca gctatcccat tcgaaggtga gcgccacaat
     1501 gcacttgatg acgcccgtta ccaggcaaaa tacgtttcag ctatctggca aaaactgatc
     1561 ccgagtcagg ctgattttta atgttcaacc atcgccggtt gtgactggta ttctgcaacc
     1621 ggccctcatc tgatgtaaga gataaaagcg atgagcgaag taatcatgat tgtctctccc
     1681 ggtaaatggg tatctgaaga acagctaatt gcactgaaag gaataaaaaa gggaacgcta
     1741 aaaaaagcca gagaaaaatc gtttatggaa ggaagggaat ataagcatgt cgcccatgac
     1801 tgtatgccat gggataacag cccatgcttt tacaacctgg aagaaataga tcgctggatt
     1861 gaacgccagg catcagcaag accaaggcgt catcttactt gagtaaaaac aatactaacc
     1921 aatgagagaa gctgaaatga aatatccgac aggcgtggaa aaccatggag ggaaattacg
     1981 tatctggttt gtttataaag gtgtaagagt ccgggaaaat cttggggttc ctgacacagc
     2041 aaaaaacagg cgcattgcag gtgagctacg cgcctctgtt tgttacgcaa taaaaactgg
     2101 cgctttcgac tatgcaaaac agtttcccgc ctcacacaat ctggaaaaat ttggtgaggc
     2161 ccgacaagat ttaaccataa aagaactggc tgaaaaattt ctggcactga aagaaactga
     2221 agtcgcaaaa acgtcactca acacgtaccg tgccgtcatc aaaaatattc tgagcataat
     2281 cggtgaaaaa aatcttgcat catcgattaa taaagaaaaa ttgctggcgg tacgtaaaga
     2341 gttactgact ggataccaga tccccaaaag taactatatt gttacacaac cagggagatc
     2401 ggctgttacc gtaaataatt acatgacaaa tctttacgcc gtgttccagt ttggtgttga
     2461 taacggttat ctggcagaca atccatttaa ggggatctca ccattaaagg agtcgagaac
     2521 catcccggat ccactttcgc gggaggagtt tatccgcctt attgacgcgt gcagaaatca
     2581 gcaagccaaa aatttatggt gtgtttccgt ttatactggg attcgccctg gtgaactctg
     2641 tgcgcttgga tgggaagata tagatctgaa aaatggaaca atgataatca gaagaaatct
     2701 ggcaaaagac cgttttacag taccaaaaac acaggcggga accaatcgtg tgattcacct
     2761 tattaagccc gcaatcgacg ctctccggag tcagatggca ctaacgagac tgagtaaaga
     2821 gcatatcatc gatgttcacc tcagagaatt cggcagaaca gagaaacaaa aatgtacctt
     2881 tgtttttcaa cctgaagtgt cagcgaaagt aaaaaattat ggtgaccatt ttaccgttga
     2941 ctcaataagg cagatgtggg acgcagcggt aaagcgtgcc ggaatccgcc atcgcaaatc
     3001 gtatcagtcg agacacactt atgcctgctg gtcgttaaca gcaggagcta acccggcatt
     3061 cattgcaaac cagatgggcc atgcagatgc gcaaatggtg tttcaggtgt acgggaaatg
     3121 gatgtcagac aataataatg cgcaggtaac gctgttaaat ac
//
LOCUS       DAZKGS010000291         3153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKGS010000291 DAZKGS010000000
VERSION     DAZKGS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..368
                     /gene="cesT"
                     /locus_tag="ACHOQY_004873"
     CDS             <1..368
                     /gene="cesT"
                     /locus_tag="ACHOQY_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP9680790.1"
                     /translation="IYYISLSDANDEYMMIYGVCGKFPTDNPNFALEILNANLWFAEN
                     GGPYLCYESGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLENE
                     HMKIEEISSSDNKHYYAGR"
     gene            429..>3153
                     /gene="eae"
                     /locus_tag="ACHOQY_004874"
     CDS             429..>3153
                     /gene="eae"
                     /locus_tag="ACHOQY_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFP9680791.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQS"
BASE COUNT          936 a          599 c          732 g          886 t
ORIGIN      
        1 agatttatta tatttcgtta tctgatgcta atgacgaata tatgatgatc tatggcgtct
       61 gtgggaaatt cccgacagat aatcctaact tcgctcttga gattttgaat gcaaacttat
      121 ggtttgcaga gaatggtgga ccatacctgt gttatgaatc aggagcacaa tcgctgttgt
      181 tagcgttacg ttttccgctc gatgatgcta cacctgaaaa acttgagaat gaaatagaag
      241 tcgtcgttaa gtcaatggaa aacctgtatt tggtattaca taatcaggga ataacattag
      301 aaaacgaaca tatgaaaata gaggaaatta gttcaagcga taataaacat tattacgccg
      361 gaagataaaa gattatttat taatataatt tactattcat tctaactcat tgtggtggag
      421 cccataacat gattactcat ggtttttatg cccggacccg gcacaagcat aagctaaaaa
      481 aaacatttat tatgcttagt gctggtttag gattgttttt ttatgttaac cagaattcat
      541 ttgcaaatgg tgaaaattat tttaaattga gttcagattc aaaactgtta actcaaaatg
      601 ccgctcagga tcgccttttt tatacgttaa aaacaggtga aactgttgcc aatatttcta
      661 aatcacaggg tatcagttta tcggtaattt ggtcactgaa taaacattta tacagttccg
      721 aaagcgaaat gatgaaggct ggacctggtc agcagatcat tttgccactc aaaaaactgt
      781 ctgttgaata tagtgcctta cctgtcttag gttcggcacc tgttgttgct gcaggtggtg
      841 tcgctggtca tacgaataaa atgactaaaa tgtccccgga cgcgactaaa agcaacacga
      901 ccgatgacaa ggctctaaat tatgcggcac aacaggccgc gagccttggt agccagctcc
      961 agtcgcgctc actgaacggc gattacgcga aagataccgc tcttggtatg gccagcagcc
     1021 aggcttcgtc acagttgcag gcctggttac aacattatgg aacggcagag gttaatctgc
     1081 agagtggtaa taactttgac ggtagttcac tggacttctt attaccgttc tatgattccg
     1141 aaaacatgct ggcatttggt caggtcggtg cgcgttacat tgactcccgc tttacggcaa
     1201 atttaggtgc tggccagcgt tttttccttc ctgaaaatat gttgggctat aacgtcttca
     1261 ttgatcagga tttttctggt gataataccc gtttaggtat tggtggcgaa tactggcgag
     1321 actatttcaa aagtagcgtt aacggctatt tccgcatgag cggctggcat gagtcataca
     1381 ataagaaaga ctatgatgag cgcccggcaa atggttttga tatccgcttt aatggctatt
     1441 taccatcata tccggcatta ggcgccaaac tgatgtacga acagtattat ggtgataatg
     1501 ttgctttgtt taattccgat aagttgcagt cgaatcctgg cgcggcgacc gttggtgtaa
     1561 actacactcc gattcctctg gtgacgatgg ggatcgatta ccgtcatggt acgggtaatg
     1621 aaaatgatct cctttactca atgcagttcc gttatcagtt tgataaaccg tggtctcagc
     1681 aaatcgagcc acagtatgtt aacgagttaa gaacattatc gggcagccgt tacgatctgg
     1741 ttcagcgtaa taacaatatt attctggagt acaaaaagca ggatattctt tctctgaata
     1801 ttccgcatga tattaatggt actgaacaca gtacgcagaa gattcaattg atcgttaaga
     1861 gcaaatacgg tctggatcgt atcgtctggg atgatagcgc attacgcagt cagggcggtc
     1921 agattcagca tggcggaagc caaagcgcac aagactacca ggctattttg cctgcttatg
     1981 tgcaaggcgg cagcaatatt tataaagtga ccgctcgcgc ctatgaccga aatggtaata
     2041 gttctaataa tgtacagctc actattaccg ttttaccgaa tgggcaggtt gtggaccagg
     2101 ttggggtaac ggactttacg gctgataaaa catcggctaa agcggatggc atagaagcta
     2161 ttacctatac cgcgacggtt aaaaagaatg gtgtagctca ggctaatgtc cctgtaacat
     2221 ttagtattgt atccgggact gcaactcttg gggcaaatag tgccagaacg gatggtaacg
     2281 gtaaggcgac cgtaacgctg aagtcggcta cgccaggaca ggtcgtcgtg tctgctaaaa
     2341 ccgcggagat gacttcgcca cttaatgcca gcgcggttat atttgttgat caaaccaagg
     2401 ccagtattac tgagattaag gctgataaaa caacagcgaa ggcagatggt tctgatgcga
     2461 ttacctatac tgtcagagtg atgaaggagg gggcacccgt agtagatcag aaagtgacct
     2521 tttctaagga ttttgggacc ctgaataaga ctgaagcaac aaccgatcag aatggttatg
     2581 ctactgtaaa attatcatcc aatactcctg gcaaggccat tgttagtgca aaagtgagtg
     2641 gagtaggtac agaagttaag gctactaccg ttgagttttt tgccccgttg agtattgatg
     2701 gtgataaagt gaccgtaatt ggtactggta tcacgggggc tctgccaaag aactggttac
     2761 agtatggtca ggttaagcta caggcaacag ggggcaatgg aaaatacaca tggaaatcca
     2821 gtaatactaa aattgcttct gttgataact cgggagtgat aaccttaaat gaaaaaggga
     2881 gtgccacaat tactgtagta tctggtgata atcagagtgc gacatacaca attaatgcac
     2941 cgggtagtat tgtaattgct gtggataaaa atactcgagt tacgtatttt gatgccgaaa
     3001 acaaatgtaa gacaaatagc gcaaatttag cacagtcaaa agaactattg gccaatatct
     3061 attcaacatg gggtgctgca aataaatatc cttactattc tggttctaaa tcattgactg
     3121 cttggattaa acaatcctct tctgaacagt cat
//
LOCUS       DAZKGS010000292         3091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKGS010000292 DAZKGS010000000
VERSION     DAZKGS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..288
                     /gene="yciG"
                     /locus_tag="ACHOQY_004875"
     CDS             109..288
                     /gene="yciG"
                     /locus_tag="ACHOQY_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9680792.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            374..874
                     /gene="yciF"
                     /locus_tag="ACHOQY_004876"
     CDS             374..874
                     /gene="yciF"
                     /locus_tag="ACHOQY_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9680793.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            920..1426
                     /gene="yciE"
                     /locus_tag="ACHOQY_004877"
     CDS             920..1426
                     /gene="yciE"
                     /locus_tag="ACHOQY_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9680794.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1486..1611)
                     /locus_tag="ACHOQY_004878"
                     /pseudo
     CDS             complement(1486..1611)
                     /locus_tag="ACHOQY_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            1835..1936
                     /locus_tag="ACHOQY_004879"
                     /pseudo
     CDS             1835..1936
                     /locus_tag="ACHOQY_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2346..2581)
                     /locus_tag="ACHOQY_004880"
                     /pseudo
     CDS             complement(2346..2581)
                     /locus_tag="ACHOQY_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2752..>3091)
                     /locus_tag="ACHOQY_004881"
     CDS             complement(2752..>3091)
                     /locus_tag="ACHOQY_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9680795.1"
                     /translation="MHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQ
                     RGVFLRNAEGSRVCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGH
                     FTVRSDCYSV"
BASE COUNT          980 a          659 c          606 g          846 t
ORIGIN      
        1 ttctcgttgc gcttaatttg actaattctc attagcgact aattttaatg agtgtcgaca
       61 cacaacactc atattaatga aacaatgcaa cgcaacggga gaaataacat ggccgaacat
      121 cgtggtggtt caggaaattt cgccgaagac cgtgagaagg catccgacgc aggccgtaaa
      181 ggcggtcagc atagcggcgg taattttaaa aatgatccgc aacgcgcatc tgaagcgggt
      241 aaaaaaggcg gtcaacaaag cggtggtaat aaatcaggca aatcctgatt caccgttaat
      301 tattaatatc agctgataaa gataattgca tctgcgggcc gtatggctcg cagtgatttc
      361 actggagaaa aatatgaata tgaagaccat tgaagatgta tttattcacc tgctttcaga
      421 tacctacagc gcagaaaaac aattaacccg ggcactggca aaactcgcaa gagcaacatc
      481 aaatgaaaaa ttaagtcagg cttttcatgc gcacctcgag gaaactcatg gacagattga
      541 acgtattgat caagttgtgg agtccgaatc gaacctgaaa attaagcgca tgaaatgtgt
      601 ggcaatggaa ggtcttattg aagaagctaa tgaggtcatc gaaagtaccg agaaaaacga
      661 agtgcgtgat gccgcactga ttgccgcagc gcagaaagtc gagcattatg agattgccag
      721 ttacgggaca ttagcgacgc tggctgaaca attaggttac cgtaaagcag cgaagcttct
      781 gaaagaaacc ctggaagaag aaaaggccac cgacatcaaa ctgactgatc tggcccttaa
      841 taacgtaaat aagaaagccg aaaataaagc ctgaaatatg aattttaact tttagtcatt
      901 ttataaagag gacattttca tgaatcgtat tgaacattat catgactggt tacgtgacgc
      961 ccacgcaatg gaaaagcaag ccgaatctat gcttgaatcc atggccagcc gtatagataa
     1021 ttatcctgaa ctacgcgctc gtattgaaca acatcttagt gaaaccaaaa accagattgt
     1081 tcaactggaa actattcttg atcgtaatga catttcacgt tcagtcatta aagattccat
     1141 gagtaaaatg gctgcgcttg ggcagtcaat cggtggtata ttcccttctg atgaaatagt
     1201 taaaggctct attagcggat atgtcttcga gcaatttgaa atcgcctgtt acacctcact
     1261 attagcagca gcaaaaaatg ccggtgatac agtctcaatt ccgatcatcg aagcgatttt
     1321 aaatgaggaa aagcaaatgg ccgactggct gattcagcat attccgcaaa caactgagaa
     1381 atttttaatt cgttctgaaa ctgatggcgt agaagcgaag aaataatacc ctttatacca
     1441 tgtccttatt gaccccgtat attacggggt cgtttttgtg cggaattaaa aacgatatcc
     1501 tgctgagaac ataaacaccc acggatcgag gcgtacgcta tcgtgttgct gtgcaccgcc
     1561 tagcttataa ttggcggtgg tatcgatatc catgtaccac actggtagat tttacaaaac
     1621 tcaataacat attatattta aaggaaaaat acagaattta cgatgctttt ttatacagat
     1681 ctctcgtgtt tttaactgat tgattttgtt gaactctttt tttgttttgc aggagttact
     1741 cctcttacct tattgtccac tcgatgacca acacattcac aacccaaacc agaatatcct
     1801 atctatgaag catgtatccg cacgcagatt aattatggtt tacattgttc taccacaata
     1861 gtcgcatcct aaatgggtgc ggttgaggat caccatcctt gaagaggatg cccgccactt
     1921 taactgcatg acagggctat ttaaacaatc actaaaaatc gcccaagagc attctgtttc
     1981 tataatgtat tccaagacat taaaaaataa tatcatacgt tattcggcag agttcgtatg
     2041 tactacggcg tcccaaccat gtcccaaaac aaaaatttct gccctataca cgttccaaaa
     2101 aattcaacag caaagagagt ctgctgacga gttgttgatt cactgattaa tacgtaaaat
     2161 tgataactgc tggaaatcat acaatactcg tactttcgaa agttcgctaa ccaggtgcta
     2221 cacgcgaggt gttgcagttt tctttacgat ttatcattca atcctaatcc atgcatgaca
     2281 tgtggtgcct gtggggtgat cctacctacg taatgtggac acagccctaa tcaaggttct
     2341 gtttttcaaa acagttccgt gctgagacag ccacaagtac tgtgtcggca ccaccgattg
     2401 taatcacact agatctaatt aaacacagcc gtctgcatta tttcccgact cataaaatat
     2461 tagccgtaaa tgcattcgac ttttagtgag tggaagaaac tttccgccct agcattatcg
     2521 taacagcgcc cctttgcact catcctgtca tgcaggtatg ccgcttcaga agcgcctgat
     2581 aatgcgttga aatcaggacg gtggctactt gctattcagt tcacgataca aaatgggcaa
     2641 tatcccttaa ggaccagcga atagcaatga gtcactttat tatcaagttt gatgaccgct
     2701 ttgatgctaa cttttaataa gagtctcttc acacaaaatc gtgtaccgag tctacactga
     2761 ataacaatca ctcctcaccg taaagtgacc gatactgcaa tcaaactcac actgctctct
     2821 ggccacaagc attgataatg ttattggctc tctgcttagt gggtgatgca tcccatcatt
     2881 aattatacga gaaagagcca tttcatcata taaactacat actctggaac cttcagcatt
     2941 tcttaaaaaa acacctcgtt gaggaattgc cagcgtaatc gggcactgta atgcttcatc
     3001 tatgtaagac atttcttcaa tattaactaa aaatgaacat gaatgtaatc gccgctgtaa
     3061 caattcaatt ctgttatcat tactatgcat a
//
LOCUS       DAZKGS010000293         3089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKGS010000293 DAZKGS010000000
VERSION     DAZKGS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..931
                     /locus_tag="ACHOQY_004882"
     CDS             <1..931
                     /locus_tag="ACHOQY_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9680796.1"
                     /translation="KELLEKVELTEDNASKLEEFSKEWKDANDKWNAMWGVKIEQTKD
                     GKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFL
                     KRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTINQNCTIKGML
                     EATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGI
                     AYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTL
                     EFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            1002..1601
                     /locus_tag="ACHOQY_004883"
     CDS             1002..1601
                     /locus_tag="ACHOQY_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9680797.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            1666..>3089
                     /locus_tag="ACHOQY_004884"
     CDS             1666..>3089
                     /locus_tag="ACHOQY_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9680798.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSAMAAGNSVKAAKT
                     SETNAKSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGVVQLSSAT"
BASE COUNT          818 a          790 c          914 g          567 t
ORIGIN      
        1 caaggagctg ctggaaaaag tcgagctgac ggaggataac gccagcaaac tggaggagtt
       61 ttcgaaagag tggaaagacg ccaacgataa atggaatgcc atgtggggcg tcaaaattga
      121 gcagaccaaa gacggcaaac attatgtcgc gggtattggc ctcagcatgg aggacacgga
      181 ggaaggcaag ctgagccagt ttctggttgc cgctaaccgt atcgcgttta ttgacccggc
      241 aaacgggaat gaaacgccga tgtttgtggc gcagggcaac cagatattca tgaacgacgt
      301 gttcctgaag cgcctgacgg cccccaccat taccagcggt ggcagtcctc cggtattttc
      361 cctgacatca gacggaaagc tgaccgctaa aaatgcggat atcagtggca gtgtgaatgc
      421 gaactcagga acgctcaaca atgtcacgat taaccagaac tgtacgatta agggcatgct
      481 ggaggcgacc caggtcagag gggatttcgt taaagctgta tcaaaagcct tcccgaaaaa
      541 agtcggtacg tggggtaaca cggaaacacc aaacggtacg gttacagtca ccatcagcga
      601 tgatcataac tttgaccgcc agattattat tccgcccatt atttttaacg gtatagcgta
      661 tgacgatccg gggagcggaa ataacccagg aggcacgcga tacacgggtt atggttttga
      721 agttcgcaaa aacggcgtat taatcgcatc cagagaaact aaaggggcca ttcccggtag
      781 ttacagtgca gttattgata tgcctagtgg tggtggtagc gtcactctgg agtttaagat
      841 tttccagaaa ggcaatcagg gggcaggcaa tatcaccgat tgtacggtga ttgtgaccaa
      901 aaaagctgct tccggcatca gtattcgttg aaatatttat aaccccaata aagggcgtca
      961 ggaatgacgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag
     1021 ccattttgtc cgcagccatc tgtctggccg tatccggtgc gcctgcatgg gcgtctgagc
     1081 agcaggccac actgagcgca gggtatcttc atgcccgtac gaacgctccc ggcagcgata
     1141 atctgaacgg gattaacgtg aaataccgtt atgagtttac ggacacgctg gggctgatta
     1201 cgtcattcag ttatgccaac gctgaagatg agcaaaaaac gcattacagc gatacccgct
     1261 ggcatgaaga ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg
     1321 tgaatgaatg gttcagcgcg tatgcgatgg cgggtatggc ttacagccgt gtttcgactt
     1381 tctccggaga ttatatccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg
     1441 gaagtgatga cggtcgccac agcaacacgt ctctggcgtg gggagctggc gtgcagttta
     1501 acccgaccga atccgtggcc gttgacgtcg cttatgaagg ctccggcagc ggtgactggc
     1561 gtaccaacag tttcatcgtg ggtgtcggtt ataaattctg attagccagg taacacagtg
     1621 ttatgacagc ccgccggttc aggcgggctt ttttgtgggg tgaatatggc agtaaagatt
     1681 tcaggtgtac tgaaagacgg cacaggaaaa ccggtacaga actgcacaat ccagctgaaa
     1741 gcaaaacgta acagcaccac ggtggtggtg aacacgctgg cctcagaaaa tccggatgaa
     1801 gccgggcgtt acagtatgga cgttgagtac ggtcagtaca gcgttattct gttggtggaa
     1861 ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattcccg acccggtacg
     1921 ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc actgcgccgt
     1981 tttgagctga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca gaacacggca
     2041 gccgcgaaga agtcagccgg cgatgccggc acatcagccc gtgaggcggc aacccatgcg
     2101 actgatgctg caggctcagc acgtgcagcc agcacatcag ccgggcaggc cgcgacgtcg
     2161 gctcagtcag cgtcttccag cgcaggaaca gcatcaacaa aggccacaga agcatcaaaa
     2221 agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcca ccagtgccgg tgccgcgaaa
     2281 acgtcagaaa cgaatgctgc agcgtcacaa aaatcagcag ccacttctgc atccactgcg
     2341 acgacgaaag cgtcagaagc cgccacttca gccagagatg cggcggcctc caaagaggcg
     2401 gcaaaatcat cagaaacgaa cgcctccaca agcgccagca gtgcagcctc ctcggcaatg
     2461 gcggcaggaa attccgtgaa ggcggcaaaa acgtccgaga cgaatgctaa gtcttctgaa
     2521 acggcagcgg aacagagcgc ctcagctgcg gcagactctg agacagcagc tgcatcatcg
     2581 gcccgtgagg cgtccattaa ggcagaggag gcagcagtca gtgccaccgc cgccggaaaa
     2641 tcggcagaaa gcgccgcatc atccgcttca acagccacaa cgaaggctgg cgaagccact
     2701 gaacaggcca gcgcagcagc gaggtctgct tccgcagcga agacatccga aacgaacgcg
     2761 aaagcgtcgg aaaccagcgc agaatcctca aaaacggctg ccgcatcgtc cgccagttcg
     2821 gcggcgtcat cggcatcatc tgcgtctgct tcaaaagatg aggcgaccag acaggcgtca
     2881 gcagcgaggg gcagcgccac gacggcatcc acgaaggcga cagaggctgc tggcagtgcg
     2941 gcagcggcag cacagagcaa aagtacggca gaatccgcag caacgcgcgc tgagacagcg
     3001 gcaaaacgtg cagaggatat tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa
     3061 aagggggtag tacagctcag cagtgcgac
//
LOCUS       DAZKGS010000294         3057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKGS010000294 DAZKGS010000000
VERSION     DAZKGS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHOQY_004885"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHOQY_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP9680799.1"
                     /translation="ENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGES
                     HLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1418..1775)
                     /locus_tag="ACHOQY_004886"
                     /pseudo
     CDS             complement(1418..1775)
                     /locus_tag="ACHOQY_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1995..2759
                     /locus_tag="ACHOQY_004887"
                     /pseudo
     CDS             1995..2759
                     /locus_tag="ACHOQY_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          767 a          776 c          786 g          728 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcaat gtactgaaac gcgacaaatg gggccttgcc
       61 ggtcaactgc gggagcttaa ggaatctaac cttatcctga attcattgct gcttacccct
      121 ctgggagaat ctcatctggt tactgtgggg ggcgagtatc agagttcgtc catgaaagat
      181 ggggttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggacgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcggggggca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagctc
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaacgcca atatcacagg ttttatgact gacttctcta acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatataaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct atggtcagag gatgtcacgc tgtcgctgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataat aaaggtgcac cgctgagtta taccccagaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcgct gagcgctgta
      901 cagaagaaag tatatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaaggtgac ggatgccctg acgctgaatg cggcagtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagtctgtac agtgccggta agagtacgct ttatgccggt
     1081 gattacttcc agacgggatc atcaacaacg ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataacaa caaaacgcta tcactgacgg tagcatacgt
     1201 tgccactgca actcctggcg gaacggtggc aatgttttag gttaagtgcg tttccggtcc
     1261 gctaatgaga tttcgttacc aacaactaat atcatcaccg gaaatgcacg gattattttt
     1321 taactcatca tttacatact tgtccagaaa tttatactcg gctagctctg aattaagggc
     1381 cgcgtccgca tctccattac cgtcttatat cactggcgct gacaattgtc tcacatatag
     1441 ctgcagtatc ggctccatcc ccgtggctac gcgctgaaat tctagtgaac cgctttcgtg
     1501 gacttgagtg aaccaactgt ttagctgtga tttatttggg gggcagaata cggtagaact
     1561 cgagtgccag ttgctgcgct attttctgtt ccggttctgt ttcacaaatc gggacgcata
     1621 attttcttcc ccctgattat tttccagggc accaccccgc agcttcccag ggacagtgaa
     1681 ggaagtcggg cggcaatagc aaacggcggg atccaacctt tacgccattt ggcaacagta
     1741 tctctgatga tagtttcagt gcctgtaact ccccgttact ttattcgtac ccttgtaatg
     1801 gggtgttagc cagccccggc atgattaatt cccccagtcg tccatgattc ggggggatgt
     1861 caccgggtct ggtggggcgc tggtaaccgc tgataggggc ctggtcagac atcatgtcgg
     1921 gacagtctgt aacgtggatg ccggtacctg ctccccaagg ttatctggtt aacccgtata
     1981 aacggggaga cacaatgccc gaatccagcg attacgaatc aatcctggtc tttattggcg
     2041 ttgatgtagg taaagacacc cattacgccg ttgccgttaa tcgttcaggt aaacgtttgt
     2101 tcgacagagt attacccaac gacgaaaaca aactcagagc gctgatatct gacctgaaac
     2161 agcatggaca gatactgctg gtcgttgacc agcctgctac catcagggcg ttacccgtcg
     2221 ccgttgcccg ctctgaaggc gtccttgtcg gataccttcc tggactggca atgcgacgca
     2281 tcgccgattt gcactccggt gaagccaaaa ccgatgcccg tgatgcggcc atcattgccg
     2341 aagctgcacg tactctgcct catgctcttc gctctctaaa actggccgac gaacaaatcg
     2401 ccgaactctc catgctttgc ggcttcgatg atgatcttgc cgcacagaca acacaggcca
     2461 gcaaccgcat ccgcggcttg ttgacccaga tacatccggc tctggagcgc gttctcggcc
     2521 cgcgactgga tcatcctgca gtacttgatc ttctccagcg atatccctca cccgaaaaac
     2581 tcgctttact gggtgagaaa aaactggcaa cccagctctg caaactcgcg ccccgtctgg
     2641 gaaaacgcct tgctgcagat atcccccagg cactgacaca gcaaaccgtc atcgtccccg
     2701 gtacgaatgc tgctgtcgtt gttctcccac gcctggcact ccagctcatc acgctgcgtt
     2761 agccaagaga cgaagtgact ctggcttcaa ctataagttt gttaatacca gtgacgctga
     2821 tgtcgttaca ggcgatagca caggacgcaa gacgatggta acggtaatag agaggcagat
     2881 gaaccatcca ctggaagatt gcggacctct cttaaaatta gtacattacg gaccgccacg
     2941 ctggaaagac agcatatgga aaggctccct gtcaaacatt ttgtttaacc cggaccctga
     3001 ccatctaacc ttggcaagtg gaagattagc gcctctttga aaaaggatca aacacaa
//
LOCUS       DAZKGS010000295         3052 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKGS010000295 DAZKGS010000000
VERSION     DAZKGS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..1892)
                     /gene="gss"
                     /locus_tag="ACHOQY_004888"
     CDS             complement(33..1892)
                     /gene="gss"
                     /locus_tag="ACHOQY_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9680800.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2097..2963
                     /gene="yghU"
                     /locus_tag="ACHOQY_004889"
     CDS             2097..2963
                     /gene="yghU"
                     /locus_tag="ACHOQY_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9680801.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          718 a          821 c          773 g          740 t
ORIGIN      
        1 tcataatcag tacggggttg ctacaacatg tattactttt tcaccacaat taacggttca
       61 atatcactct cttttttgat gaccagtgat tcatcaccgc gcaaacacgt cccaccgtag
      121 ttgccgccaa cggtgaaggt acatacctga atgtatttac cgtccacttt cggcaaacac
      181 cacagttgct gatagatgtt tttctgctcg gcaaatttac cgctggtttt gtccagcacc
      241 tcttcatgat ggctgaccag gttgatattg ctaccacagc gaccggcaat cggtttcact
      301 gcgtaacctg ttttcaccaa ttcatcatta acagtgaaat cggtatccag caggtaacga
      361 tggtgcggga acagcgacca gaggatcggc agaatcgctt tgttgccggg gatcaccgtc
      421 cacagcggct caaagactag cacttccggg cgcagcaata cgtcgataag acgcacttcg
      481 ttttgcggat gaccggtacg gattggcacc gcagcaaact cacggtcgct aacttcacga
      541 atctgatcaa acgcggtttc ccacgcccag gttttccaca cgcagttaac cagccgtcct
      601 tccccatcaa tcagttgccc ggcagcatcc cagcccagtt catccagccc gcgcaagata
      661 cgcgtttcaa agccctcctg gtgcagcgcc tgctccataa actgcgcgtg atagttttcc
      721 tcgatatctt tgtcctgcat gatatggaca aacggacgtg cacgactgtg tttccaggca
      781 ccagccagtt cgttaatcag cccttccgcc ggattgaagc cgttgccttt atagccctgc
      841 tccgcccaac gttcgaggat caagcccgct tcggtatgac atgaggcaga gtcggcgttg
      901 tattcgtaaa ccttcaggcc acgctcatcc atgcagaaat ccatacgacc agtgatcata
      961 tggtgacggc gacgctgcca ggagagacgc aaacgtggcc agaggatttt cgggatgtcg
     1021 aacagcgcca gcaggttgtc atctttcagc accttgtcgg ttgcgtgaag atacatcagg
     1081 tgcagctcgt tggtggcttt aattagctcc tgctcggcac tctcggtaat ggtgtagtaa
     1141 tgataaggat cctgattgat cacctgaccg ttggcctgca cataggcgtt ttgcagcgga
     1201 tctttttcat ccagccattt accgtcaaac tggcctttgt tttccaggcg cgctccgctg
     1261 attttcagca gctcgcctgc aatttccggc tgcggtaagc tgtattcagt atcttccgtc
     1321 tggatcatcc agcccagaat ggtggtgtca tcaaaagtgt ctttcagggt atagcagccg
     1381 ttttcgacca ccatctccag ctcacgcgtc cactgttgcc cttgcggcaa cggggaatga
     1441 atcacgttct gttccgcaat acggactttg ttgccatgca attgggtaat gatggcgaca
     1501 tggccagtgt ctttaaattc accgccttta tcccagataa gaagcgcacc cgcgaccggc
     1561 gcacgcggcg agccgttagg aaatgcctgc aatggcagga tgttgtcatt aaccacttct
     1621 cgcaggaagc gcagcgagaa aatctcccac gccataccca cgtcagtaaa gaccacaccg
     1681 taattcagaa agagaaaacg gcgagcaaat tcaacgcatt gccacttgtg gcccatatat
     1741 tcgtcgtcga tatagctacg gaatacggcg tcatcttcgt attcctgcgg atcgagagaa
     1801 ctgtaatctg aagagtagat tgctacccca cctggggcgt agcccaataa tgtcccgaac
     1861 ggggcatcct ggctggtcgt tcctttgctc atcactttac ctttaacaat acaacctaag
     1921 cagttggcgt aattgttgtg ctctgattac ctgctcagcg acattaaccg gacagaggtc
     1981 agactaatca tacacctcat cgcagcggct ggcgcaaagg gttaaaaaat tcacattctg
     2041 tacagcaagt gacctgctac actgcttcaa cactaccact cagaaggcaa ctcactatga
     2101 cagacaatac ttatcagccc gcgaaagtct ggacgtggga taaatccgct ggcggcgcgt
     2161 tcgccaatat caatcgcccg gtttctggtc cgacgcatga aaaaacgctg cccgttggca
     2221 aacacccatt gcaactttat tcgctgggaa cgccgaacgg tcagaaagta acgattatgc
     2281 ttgaggagct gctggcgctg ggcgttactg gtgcagagta cgacgcctgg ctgattcgta
     2341 ttggcgatgg cgatcaattc tccagcggct ttgtcgaagt gaacccaaac tcgaagatcc
     2401 cggcgctgcg cgatcatacg cataatccgc cgatccgcgt gtttgaatct ggttcgatcc
     2461 tgctttatct ggcggagaaa tttggctact tcctgccgca ggatttggca aagcgtactg
     2521 aaacgatgaa ctggctgttc tggttacagg gcgcggcacc gttcctcggc ggtggttttg
     2581 gtcactttta ccattacgcg ccgatgaaaa ttgagtacgc catcaaccgc tttaccatgg
     2641 aagccaaacg tctgctcgac gtgctggata agcaactggc gcagcataag tttattgcgg
     2701 gcgatgagta caccattgcg gatatggcga tttggccgtg gtttggcaat gtggtgttag
     2761 gcggcgtgta tgatgccgca gagtttcttg atgcgggcag ttataagcat gtacaacgct
     2821 gggcgaaaga agtaggcgaa cgtccggcgg tgaaacgtgg gcgtattgtt aaccgcacca
     2881 acggaccgct gaatgagcag ttgcatgagc gccatgacgc cagtgatttc gagacgaata
     2941 cggaagataa gcgtcagggg taagggttgg tgttcgtcgc agcaagccat ccaggccgga
     3001 taaggcgttc acgccgcatc cggcaatcgt gcacaatgcc tgatgcgacg ct
//
LOCUS       DAZKGS010000296         3046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKGS010000296 DAZKGS010000000
VERSION     DAZKGS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..173)
                     /locus_tag="ACHOQY_004890"
     CDS             complement(<1..173)
                     /locus_tag="ACHOQY_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680802.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTV"
     gene            complement(163..342)
                     /locus_tag="ACHOQY_004891"
     CDS             complement(163..342)
                     /locus_tag="ACHOQY_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680803.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(518..1075)
                     /gene="ymfL"
                     /locus_tag="ACHOQY_004892"
     CDS             complement(518..1075)
                     /gene="ymfL"
                     /locus_tag="ACHOQY_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP9680804.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(1119..1319)
                     /gene="ymfT"
                     /locus_tag="ACHOQY_004893"
     CDS             complement(1119..1319)
                     /gene="ymfT"
                     /locus_tag="ACHOQY_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP9680805.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1410..2084
                     /gene="ymfK"
                     /locus_tag="ACHOQY_004894"
     CDS             1410..2084
                     /gene="ymfK"
                     /locus_tag="ACHOQY_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9680806.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            2753..>3046
                     /gene="yfdP"
                     /locus_tag="ACHOQY_004895"
     CDS             2753..>3046
                     /gene="yfdP"
                     /locus_tag="ACHOQY_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP9680807.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQ"
BASE COUNT          815 a          763 c          711 g          757 t
ORIGIN      
        1 ctgacggtac tcatccccgc gccaatctga cgggcaatgg tttcaattga tggccagcac
       61 acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct ggataatttc
      121 atgcctgacg ctgcgcaacc atcccataca tagccggtta atttagtgct catgaccgac
      181 ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact catgctcata
      241 gccttcacgg aggtagataa cccgttgtgt ttccggctcc caacgaatga ctctgacggg
      301 cactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt ctccttctgc
      361 cggttgaaat cccccacagc ccactctgca aagctgtggg ttacaatttc cctgtcacct
      421 ggtacattta ctgcatagca atactccacc ttcgcttttc cacccggtac aggaagcgca
      481 atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt tctccacaac
      541 cagaagcaat cgacgccacg acgcccggag ctgcacactc gcgggcgtca ttactttctg
      601 aaacgcaaaa aattttgtag acaagtgctg catgctcctg cagcttcgaa attgagaggt
      661 acagctcgtc gttaattgct gtcttctcat gcggttccac tacaccgtct tcgattgccg
      721 aacgaatctg ttttgaataa ctgccgatct gttcaatgac ttccagtaaa cgctggttaa
      781 tatcggcatt gtccacatcc tcgacgtcag gaagagacac aaagacgcca tttgcagact
      841 gcgccacagc gtcagcaatg aagtgagtgc caccagcacg ttgtaaaatc attgcccatc
      901 ccagcgggaa aatctgatcg ccatcggcac gaaggcggtt aaataatgcg ttctctgtta
      961 catccagcca ctcagcagct tcagcgtaac cccccggcaa cgctgcgata gtttttctga
     1021 cagctttcac gtaccactca ggctgttttt ctactttcca gtgatactta cccacggtta
     1081 gcctcatcgt tctgtggtta aaaattgaag gtgttctgtt aatctttcgg atagatatcc
     1141 ggtcttaagt cagatttcgt aattgcacct gacgtgcatt gctcaagttt tttcgccagc
     1201 acaaaactgg cttttttata accattgaaa accagccgta agtagccagg tgttgagcca
     1261 acttttccgg ccaactcgcc ctgctgttct ttggttaaag agtcccaata cgctttcata
     1321 caacatgtac ctccggtata catattacat gattgaaatg aaccttcaag atacttgtac
     1381 cttatcggta caaaggtttt aatttcgtta tgaaaacaat ccatgacatc cggcggtcta
     1441 acgccagaaa actgagagat ggtgttggcg gaaattcatc ctttgccacc atgattgatc
     1501 gcgagccaac ccaaaccagc aggtttatgg gggatggcgc tactaaaaat atcggtgaca
     1561 gcatggcacg ccacatcgaa aaatgtttcg acctgcctgt cggatggctt gatcaagaac
     1621 accagacaac gaacatcaca aaaaaacctg atgtttcaat cactaacaaa caaataacgt
     1681 tagtccctgt catatcatgg gtacaggccg gagcatggaa agaagttggc tattctgagg
     1741 ttgatttgag cacagcagaa acgtatccct gccctgtacc ctgtggcgaa atgacttata
     1801 tcttgcgggt gattggtgat tcaatgattg atgagtaccg ccctggagac atgatttttg
     1861 ttgatcccga agtccctgcc tgccacggtg acgacgttat tgcattgatg cacgatacag
     1921 gcgaaaccac cttcaagcga ttgatagaag atggaacaca gcgttatctc aaagcattaa
     1981 acccaaactg gcctgagcct tacattaaga ttaacggtaa ttgctctata attggtacag
     2041 tgattttctc gggaaaacca agaagataca caataaaggc ctaatcaata tttatgaacc
     2101 tgcttcggca ggttttttta tacttgacaa tgtacccatg agatacataa tgtatccaaa
     2161 agaaacatga ggcaggcaag attcaaacaa aatttggttg taacacggcg tatggcacat
     2221 gcgtcgttag cggtctgggg acgttaaagg ggacaatcca ctccttgcta gggcaaacaa
     2281 accaggtagc cggaatgtgc aagtcaatga tgatgctgat aagacgccta accagcgtgg
     2341 cgatccggtt tgacgcctgg gaagagacca gggtgcaacg atgagggcat ttatggagcc
     2401 gcgacaaagt gtggtgccgt aactggctaa gtgctctcag cgttgtggtc atccgcgaaa
     2461 tggcgcggcg gtaagtatgg cggggttact ctttccccgt tgaggacacc ggattgtcag
     2521 gttgaccata cgcctgagtg acaaccccac cacaacagcc actgctttgg cggtaccagt
     2581 ttgtacactt gcttccggct ggtaccgctc tttttacaaa acagagaaga gcatcaccgg
     2641 acgacgggct cataacccaa tccacccggg cggctgccac cgcaggtgct cttctctgtt
     2701 ttgtggagaa accaaccgac cttgcagggt cgatatgatg aggagcagca aaatggctag
     2761 cgaacgcagt actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac
     2821 caaaatcggc gctgttctca gtgaagtcgc ttcaggtgtg atgaacacga aaaccaaagg
     2881 gaaggtctcg ctcaacctgg aaatcgaacc gtttgatgag aaccgtgtga aaataaaaca
     2941 caaactctca tatgttcgtc cgactaaccg cgggaaaatt tccgaagaag acaccaccga
     3001 aacgccgatg tatgtcaatc gcggtggtcg cctgactatt ctgcag
//
LOCUS       DAZKGS010000297         3042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKGS010000297 DAZKGS010000000
VERSION     DAZKGS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(208..810)
                     /locus_tag="ACHOQY_004896"
     CDS             complement(208..810)
                     /locus_tag="ACHOQY_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680808.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(897..1103)
                     /locus_tag="ACHOQY_004897"
     CDS             complement(897..1103)
                     /locus_tag="ACHOQY_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9680809.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1855..2004
                     /locus_tag="ACHOQY_004898"
     CDS             1855..2004
                     /locus_tag="ACHOQY_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP9680810.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2816..>3042
                     /locus_tag="ACHOQY_004899"
     CDS             2816..>3042
                     /locus_tag="ACHOQY_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9680811.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          784 a          730 c          816 g          712 t
ORIGIN      
        1 tgcgtggtgg tgatgaatca acaggcatat aactgatacc cgccggtcag aagttccagc
       61 ctgcgacttt cctgtctgcg tgcccgcagt ccggcgttca ggtgcagcgt gtgacagaac
      121 gcacggagag cgaaacggca ggcagacagg ccgttcagtc agccctggca ccctgcccgg
      181 ataccagctt ccctgaaaca actggcatca ggtgcgctta tcgtcaaccg ttgtgcggta
      241 tgccaggagc acgtactgat ccatcagatc cgtcagtgct gcgctctgct tctgacggtt
      301 gatgatccat tcctccagct ctttttctga tttccgtttc agattctccg tccagtcgtc
      361 aaggcagttt ccggtcatta aatcaagcca gcaaagccag accagtttga ggcgcaactc
      421 cgtgccacca aactgacgga agaggctgat cagtttattt gccagcgaac gctgcctcag
      481 acgttcacat tcagacagga gaatgccttc cagcatcgga tgatgaacca gcagatgttc
      541 actggtgcat aaattcacta tcagatcatc atccaggggc ggcgtggtcc gtttgtccgg
      601 accataaatc agccggtgca gactctgggg tacgatggca atctccggtt tttcaccatt
      661 gaatgacacc cacccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      721 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcag
      781 ctcgtaacgg gtctgatact gtggtttcat tttcatattc ctcacggggg ataacaggca
      841 gcagaacgcc gccggcaggc cggagactgc gtctgctgca gatgaacggt taagaattac
      901 ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcgctttt cagccatcgg
      961 gagctgcggc ccattttgat gggcgcagga aagtcaccat ccttgatgag tttgtaaaac
     1021 cacttatcgg tcaggccggt cagttgagtg ataaatgcca tgtcgaccat ctggtcatcc
     1081 atcagtgaaa ctggggtgat catgagaatg ttcctccgga tgttgttaaa ccagccggtg
     1141 tggtcatgaa aacaacgcac caccttcacc ggcataacaa ccggggagaa acgtaaaaaa
     1201 taatcgggaa tgtgaccgcc aggagataaa cggggaggtt acggtggctg agccgtaccg
     1261 gtatgccggg tggttgttac gaaacgatgc ttccgcacgc gcgcgcgata aagaagggat
     1321 gattttcgtg aggaagaggg gaaaactggc gttctgataa ttgcagagtt atttacgggg
     1381 cggttacgat ggggcagacc gtgtccggtg ggcaggcgac aggtatcacc gatgggataa
     1441 gactgtctga ttggtattat aaatatataa gagagatcaa acacatgaga tcaaatacat
     1501 gagttcatat atgtgaaata aacacggtga gtgtgatcag tgggcatgat gagaacgcag
     1561 tagcaacaga ctgcgctatc cctgatagct ggcgtataac ctgcgtatat aaaacgtacc
     1621 agcaacgcac gtgaacagag tgcattaagc atccgtcgtt gacaccgacc tggtgagtga
     1681 cagtctgaga gaccggttaa gtggtggtac gggagtaatg agtttgggga gggtaccaga
     1741 atgtcagtct ggtacggtat gctgacagta tcacgttaat tgcagcataa agtgatgaga
     1801 ggctaatgga agttaaaacc agagcagttc cgtcgttgtt catcccggga gacactggaa
     1861 aaagtgattt cccacacgcg ttataagctt actccagcgg agatggaagc cttcaactct
     1921 gcggtcgatc accgactggc agaactgaca atgaacaagc tttacgatcg tgttcccgct
     1981 tcagtctgga aatatgtcat ctgagcgttc ggtaatcaca actgcatcgc gctaggtgtt
     2041 atccgctttg tgattcttat tggctcttct caggttatta aaagctgaat atgttcctgt
     2101 tgtcgggaac caggataata ccgggagcgt taagcggtat agcgacaaca aagaattcga
     2161 aatcaaaagg gaatggtgcc ggaggcgtgg gcggtaatag gctgattttc aggtattacc
     2221 ggcttacaac gtgtggttga tattgagggg ctgtgagttt cgcgtatttg tcagaaaccc
     2281 agatgagttt tcctgtatgg gacgaaataa ggcagcggca atacctggaa aatttaatat
     2341 ggtatgaagg agaacctgtc ggttacgctt aatatacacg gtacactgtc agctcagggg
     2401 atacatgaac cgttatctca ccagctacac agtatacaca cactgacgct gacagtagtc
     2461 agcaggacca caataatcag cgccagcagt atggtaacga tacggtctgt attccggtag
     2521 tagccgaagg ggtcgacaga accacaatac gggcaacaca ctgtattatt agtaatcgcg
     2581 tttccacagt ccctgcactt cttttgcgta tcaccataac tccctcctca cagccttacc
     2641 cgtaaccggt tttttacatt aaaaaacaac gctgaaaatt caaccgtcag tccggagacg
     2701 accgttcggg ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca cttcagggac
     2761 tctgcgcatt ttttacgacg tggctattcc gtatgaacca tacggagatt taaccatgac
     2821 ctacaaatac aaccccttct ggcagcaacg tattcgtgag acggtgcggc acgcactgga
     2881 tgttcatccc cgtctgacgg cattgcgggt tgatctgcgt ctcccggatg taccggcagc
     2941 aacggacgca gctgtgatat cccgcttcat caatgccctg aaagcccgaa tcgacgctta
     3001 ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc ac
//
LOCUS       DAZKGS010000298         3031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKGS010000298 DAZKGS010000000
VERSION     DAZKGS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            426..992
                     /locus_tag="ACHOQY_004900"
     CDS             426..992
                     /locus_tag="ACHOQY_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9680812.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            967..1569
                     /locus_tag="ACHOQY_004901"
     CDS             967..1569
                     /locus_tag="ACHOQY_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9680813.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            1566..2231
                     /locus_tag="ACHOQY_004902"
     CDS             1566..2231
                     /locus_tag="ACHOQY_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP9680814.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            2228..2851
                     /locus_tag="ACHOQY_004903"
     CDS             2228..2851
                     /locus_tag="ACHOQY_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9680815.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT          934 a          603 c          731 g          763 t
ORIGIN      
        1 tagtctaact atagttatca ccaaaaactt gcctcgattt tagatttttc cagtatttgt
       61 agatattgca ctgaacaccg aatacgtagc agagggtgtc tacacgataa cgttctatga
      121 gctaccatgt tgtcgaaaaa ttgtttagtg agtatgacat caggaatgtg gtggtctgtt
      181 ttaatatttc tatttctatt tctatttcta tttctatttc tatttctgtt tgttgtggtt
      241 ttttctttag ttcatgtatt tcgatttgtt tctgaattat cgggggtagt gtcttccctt
      301 ttccctgagg cttatcatgc agttattttc gccatcttgg tattgtggaa ataccgacat
      361 ccatagcttt ggtgagctct gttcaggtgg ccaaattcag aaaaccatta cggaggaaga
      421 aggcgatggc taaaccagcg cgaagacgat gtaaccgtaa aagagaagat ttaactgtta
      481 aaaggatatt tgagttacta agtttcgata aatctaccgg ggtatttaga tggaaagttc
      541 ccactcaggg aaggatagca ttaaatagtg ttgctggaac ttttgattcc aacggttatt
      601 caatgatcat gatagatggg cgtagatata aaactcacgt cttagttttt tacataactc
      661 ataatcgttg gcctgctggt caaattgacc acgttaatgg aattaggacc gacaataggc
      721 cagaaaattt aagagaatgc ctgccaatag aaaattcaag aaatataagg atccgaaaga
      781 atagcaaatc aggttgcaga ggggttactt ggcacaaacg acagaaaaaa tggaatgtta
      841 ggctaggatt ccatggcaag agtaaacact tcggatgctt tgatgatctg gagttagcgg
      901 tactagttgc tgaagaagcc cgagataagt attacggtga tttttccggc aacgaaagga
      961 gcacttatgc gaatctatcg aaggaaatgt aaatgttgca atgaatggtt tataccaaaa
     1021 tatcaaaatc aatattggtg taatgagatt tgtggaacca agatagcact cgaacgacga
     1081 agtaaagaac gcgaaaaagc ggaaaaagca gcagagaaga aactacgacg agaggagcag
     1141 aaacagaaag ataaactgaa gattcgaaaa ctcgccttaa agccccgcag ttactggatt
     1201 aaacaagccc aacaagccgt aaacgccttc atcagagaaa gagaccgcga cttaccatgt
     1261 atctcgtgcg gaacgctcac gtctgctcag tgggatgccg gacattaccg gacaactgct
     1321 gcggcacctc aactccgatt tgatgaacgc aatattcaca agcaatgcgt ggtgtgcaac
     1381 cagcacaaaa gcggaaatct cgttccgtat cgcgtcgaac tgattagccg catcgggcag
     1441 gaagcagtag aggaaatcga atcaaaccat aaccgctatc gctggactgt cgaagagtgc
     1501 agggccatca aggcggagta tcaacagaaa cttaaaaaac tgcgaaacag cagaagtgag
     1561 gttgcatgaa tatctacgaa agaattgatg gcagcaaata ccgaaatatt tgggtagttg
     1621 gcgatctgca cggatgctac acgaacctga tgaaaaaact ggagacgata ggattcgaca
     1681 ccaaaaaaga cctgcttatc tcggtgggcg atttggttga tcgcggtaca gagaacgtag
     1741 aatgcctgga attaatcaca ttcccctggt tcagagctgt acgtggaaac catgagcaaa
     1801 tgatgattga tggcttatca gagcgtggaa acgtcaatca ctggctgctt aatggcggtg
     1861 gctggttctt taatctcgat tacgacaaag aaattctggc taaagctctt gcccataaag
     1921 cagatgaact tccgttaatc atcgaactgg tgagtaaagg aaaaaaatat gtcatctgcc
     1981 acgccgatta tccttgtgat aaatacgagt ttggaaagcc agttgatcat cagcaggtaa
     2041 tctggaaccg cgaacgaatc agcaactcac aagacgggat cgtgaaagaa atcaaaggcg
     2101 cggacacgtt catctttggt catacgccag cagtgaaacc actcaaattt gccaaccaga
     2161 tgtatatcga tactggcgca gtgttctgcg gaaacctcac attgattcag gtacagggag
     2221 aaggcgcatg agactcgaaa gcgtagctaa atttcattcg ccaaaaagcc cgatgatgag
     2281 cgattcacca cgggctacgg cttctgactc tctttctggt actgatgtga tggctgctat
     2341 ggggatggcg caatcacaag ccggattcgg aatggctgca ttctgcggta agcatgaact
     2401 cagccagaac gacaaacaaa aggctatcaa ctatctgatg caatttgcac acaaggtatc
     2461 ggggaaatac cgtggtgtgg caaagctcga aggaaatact aaggcaaagg tactgcaagt
     2521 gctcgcaaca ttcgcttatg cggattattg ccgtagtgcc gctacgccgg gcgcaagatg
     2581 cagagattgc cacggtacag gccgtgcggt tgatatagcc aaaacagagc agtgggggat
     2641 agttgctgag aaagagtgcg gaagatgtaa aggcgtcggt tattcaagaa tgccagcaag
     2701 cgccgcatat cgcgctgtga cgatgctaat cccaaacctt acccaaccca cctggtcacg
     2761 cactgttaag ccgctgtatg acgctctggt tgtgcaatgc cacaaggaag agtcaatcgc
     2821 agacaacatt ttgaatgcga tcacacgtta gcgccatgat tgccacggat ggcaacatat
     2881 taacggcata atattgactt tttgaataac tttggggaaa cttgacacca ataatgggcg
     2941 ttttttacat gtcattgatg agtctcaata acctgccgcc gagtagtttt tatgctctga
     3001 attgtatttg tgtagtaaac atgctgactg c
//
LOCUS       DAZKGS010000299         3017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKGS010000299 DAZKGS010000000
VERSION     DAZKGS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..913)
                     /locus_tag="ACHOQY_004904"
     CDS             complement(56..913)
                     /locus_tag="ACHOQY_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9680816.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            1162..2031
                     /locus_tag="ACHOQY_004905"
     CDS             1162..2031
                     /locus_tag="ACHOQY_004905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9680817.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(2191..2784)
                     /gene="rclC"
                     /locus_tag="ACHOQY_004906"
     CDS             complement(2191..2784)
                     /gene="rclC"
                     /locus_tag="ACHOQY_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9680818.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2796..>3017)
                     /gene="rclB"
                     /locus_tag="ACHOQY_004907"
     CDS             complement(2796..>3017)
                     /gene="rclB"
                     /locus_tag="ACHOQY_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9680819.1"
                     /translation="VLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIAD
                     IARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT          871 a          617 c          666 g          863 t
ORIGIN      
        1 ttattgaatt tcctgacagt agtctcgtta taaattcatt tagatatagc gaaaactaaa
       61 ccggacgaac ggggataaaa atttcgcaaa caatgttatt gctatcaatt tcgacgaaat
      121 tttcattgta ataaaaccgc tcaatatcac tgccgttcac tctggctaac cccctggcgg
      181 gtaattccat cagatagagg tttcgcgccc attcagggta gtgctcccac tcaccttcat
      241 agcggaaaga ggcatacagt cctcggttaa ttgtccactc tttcggcatc tctgaatcgg
      301 tttgtttgca taccgtaccg gcaaaagttt caaccgtgag caaatcgacc gattttgttg
      361 acggttcaaa cgtggagacg aaatagatat ccgtcttaaa attattcata catctggcta
      421 aatgatgacg tcgcagtttg acttcttcat ccggtgattt attcgacact tcaatatcat
      481 atttaaatga attgccaaaa ataggtgtct caggaaagtt gactaatcta cactcggtct
      541 tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa taatcacggt
      601 ggcggtatac acggggagag cagccgaata actttttaaa ttcgcggctg aatgactgct
      661 gtgaatcaaa atgcaaagag agtgcaatat caagcataga tttcgcggtg agccggacaa
      721 gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc acatgcatga
      781 aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt gcgatatctt
      841 cgattgaaat agggtgctca agattgcact caatccactc caggagctgc tgtagaatct
      901 tctgcctgat catcacattc tccattgatt cttcgtcata tcgcacattt tcgttatgcc
      961 ctgggcccta agtgtatcaa aaacagcaag ggcgaaggca aaagcactgg tagcgtctgc
     1021 ttcgttgtcc ccgaaaatag agaggtttca gtcctgcatt attaatgaat tttttgcata
     1081 agtgatatca aaatccacgt actaatttga ggttacgttt taacgtagac tcattgttca
     1141 tgcctaatgg agggactgac agtggaattt tccgtattaa gtaacaatct gaaaatgccg
     1201 atgatgggat ttggtgtttt tcaggttacc gataaaaacg tgtgcaaaca gtcagtgttg
     1261 aacgctatcc gcacaggtta tcgactcatc gacactgcag cggtatacgg taatgaagat
     1321 gccgtcggtg aagccgttcg tgaagctata tctgaaggtt tatgtacccg agatgagtta
     1381 tttataacgt ctaaattgtg ggtgcaggat atgttgaatc aggatacggc agcagcaggt
     1441 attgaagcat cattaaaaaa atcgggacta gagtacttcg acctctattt attgcaccag
     1501 gctatgcgcg attatttcag tgcgtggcgt gcactcgaag atgcctatga agagggcaaa
     1561 ttaaaagcaa ttggggtttc caatttctat cctcatgttc tggcgaactt ttgtgaaacg
     1621 gtaagagtta aaccgatggt caaccaggtc gagttgcatc cctattttgc ccaaccagag
     1681 gcgctggcaa ccatgaagta ttataacgtg cagcctgaag catgggctcc gttaggtggt
     1741 ggacgacata aaccctttga aaataatctg cttcagagta ttgcagatgc ccatcaaaaa
     1801 tcgatttctc aagtcattct gcgttggaat attcaacggg gagtggtcgt tattccgaaa
     1861 tcgacacatc aacagcgtat cgaagaaaat tttgctatct gggatttctc actgacagag
     1921 aaagaaatgg cacaaattag ttcgcttgat ttgggttatg ttggggagtc ggtaaaacat
     1981 tttaatcctg aatttgttcg tggttgtctt gctgtaaaaa tacatgattg atattaatca
     2041 tatattttac ctgagacgac aagaatcttt taacagggga gtgatattga tcttcactct
     2101 gtcatatctc cggtaatatg gcgtcaggct tcatcatcag aaagggggcc tgacgcctga
     2161 aaaagtgaac aacagacagt gttcggatta tcaatattca gtttttaacg ttgaactgga
     2221 ttcattactg cgttgtttaa gaatttcccg cgccgaatct gccattatca tgacagcacc
     2281 tgccagcatc agagtatctt tcaataccag gcgaccagca ccggataaat aagggaaacc
     2341 atgatgagcg tcacccaatg cgggtaccca tgcctccggg gtggtgatta aaaatgagag
     2401 tgttaccagc ggcgtggtaa atgccatcag tcctcccaat aaacctaacc agcgattgac
     2461 aggattagcc aaaaccagca gagcaataat cacctccacg acgccaagac cgttggaaaa
     2521 accataggta ttattggccg tttgccatgc ccttgcttct ggtttgtatt cgccttcgtg
     2581 agtcagatac tgtttatagt cttccgggtg ttcataaaag aacgacatta gtggactgtt
     2641 tgcgacgaat ggtgtaatgc tgtctgcctc gtaagggaca aactttaata acccaatcca
     2701 cataaaaaca attgcaatac tcagacgaat caatgtcagg ccaattttat ctccccgact
     2761 taacaggtgc aggtattttt ccattgtgaa catccttatt tatacaatac tgctgttgcg
     2821 gttgaattat tatcaattcg catcgatgtg actttccagg atgaagcatt atattttctg
     2881 gctatatcag caatggtatc ctccagaact gtcggtgatg ctgatactga cgagacgctg
     2941 atatgtttca gaattatttt atcatctgca ttggtggaaa atgccataac gccagataat
     3001 agtgttgcaa ataaaac
//
LOCUS       DAZKGS010000300         2973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKGS010000300 DAZKGS010000000
VERSION     DAZKGS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            226..1437
                     /gene="bluF"
                     /locus_tag="ACHOQY_004908"
     CDS             226..1437
                     /gene="bluF"
                     /locus_tag="ACHOQY_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP9680820.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1642..2373
                     /gene="bluR"
                     /locus_tag="ACHOQY_004909"
     CDS             1642..2373
                     /gene="bluR"
                     /locus_tag="ACHOQY_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP9680821.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2594..>2973
                     /locus_tag="ACHOQY_004910"
     CDS             2594..>2973
                     /locus_tag="ACHOQY_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9680822.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDV"
BASE COUNT          869 a          627 c          635 g          842 t
ORIGIN      
        1 attttttgca attagtgcta atgcatataa aacaatgtgt tatgataaaa catattgcat
       61 catttaactg acaatctata caagattttt cgccaactag tgttcaactt tttccatttt
      121 ttatcaatgt aaagaaacgg ttatgttttg gtattaaaag aaagacacaa gaagatatgt
      181 ctgttaccgt cttactctcg cctcacccat taccctggat tgttaatgct taccaccctt
      241 atttatcgta gccatatacg tgacgacgaa cctgtcaaaa aaatcgaaga aatggtttcg
      301 atagcaaatc gcaggaacat gcagtctgac gtaacaggga tcttactgtt taatggttct
      361 cattttttcc agcttctgga aggtccggaa gaacaggtta aaatgatata tcgggctata
      421 tgccaggatc cacggcacta taatattgtt gagctgctgt gcgattacgc gcctgctcgc
      481 cgttttggca aagcgggaat ggaattattt gatttgcgcc tgcacgagcg agatgacgtt
      541 ttacaggccg tattcgacaa aggcacatca aaatttcagc taacttatga tgacagagcg
      601 ctacaatttt ttcgtacttt tgtccttgca accgaacaat caacctattt cgagatccct
      661 gccgaagact cctggctttt tatcgctgac ggatctgata aagaacttga ttcctgtgcc
      721 ctttcaccaa ctataaacga ccactttgcc tttcatccta ttgtcgatcc cttatcgcgg
      781 cggataatcg cttttgaagc cattgtgcaa aaaaatgaag atagcccatc agccatagcg
      841 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      901 ttcacgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      961 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttaatgaaat aaaggccaat
     1021 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagtcat atctcggttt
     1081 gatgagtttg ccgaagcgat taaatcgcta aaggctgctg gtatcagtgt agcaattgat
     1141 cattttggcg caggttttgc tggtttgtta ctcctgtcac gcttccagcc tgacagaatt
     1201 aaaatcagtc aggaattgat taccaatgtt cataaaagtg ggccacggca ggcaattatt
     1261 caggcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1321 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1381 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1441 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttacgttta
     1501 aatttaatcc acattaaaaa taatgtttca tccaatttag ctgaaatctt atttatactt
     1561 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cgacggagat
     1621 ttgtgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1681 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1741 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1801 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1861 agccaggata cggaagatga ctggagccgc ctgcaagaag agatgatgtc aattcttcgc
     1921 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1981 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     2041 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatacgc agcaacctcg
     2101 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     2161 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     2221 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2281 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2341 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2401 gaatgttata atctgaaccc acaacagcgt ttgtaccgga gctggtccgt tgcaaaatgc
     2461 tcaccgggag aattcagata ttccctctat cacactctgt acatatcaca gccaaaggac
     2521 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2581 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2641 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2701 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2761 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2821 ggccgcattg cttcatattt gcggaaaatc tacatttcgg gaatactgtt caacactagc
     2881 aggcgcgggc gttttccggt gggttactga tgttaatcat aacaaacgca gttactacgc
     2941 cattgataat acgcttttat atattgaaga tgt
//
LOCUS       DAZKGS010000301         2936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKGS010000301 DAZKGS010000000
VERSION     DAZKGS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /locus_tag="ACHOQY_004911"
     CDS             <1..517
                     /locus_tag="ACHOQY_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9680823.1"
                     /translation="YGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRDGAGIWT
                     ICRGATRVDGKPVLPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQKAGIASF
                     CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQ
                     ESALACWGIDR"
     gene            810..941
                     /locus_tag="ACHOQY_004912"
     CDS             810..941
                     /locus_tag="ACHOQY_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680824.1"
                     /translation="MSDKLITPAKVLCVIVGISFSLMLVALFLSLAWVMLSSSGLLG"
     gene            905..1060
                     /locus_tag="ACHOQY_004913"
     CDS             905..1060
                     /locus_tag="ACHOQY_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680825.1"
                     /translation="MGDVVFVGAAGVTVTDDISRALAFAIKWVAVGIAVSPMLYGLAK
                     LVIALKS"
     gene            1072..1158
                     /locus_tag="ACHOQY_004914"
     CDS             1072..1158
                     /locus_tag="ACHOQY_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680826.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            1380..1565
                     /locus_tag="ACHOQY_004915"
     CDS             1380..1565
                     /locus_tag="ACHOQY_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9680827.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            1966..2292
                     /locus_tag="ACHOQY_004916"
     CDS             1966..2292
                     /locus_tag="ACHOQY_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP9680828.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(2424..2624)
                     /locus_tag="ACHOQY_004917"
     CDS             complement(2424..2624)
                     /locus_tag="ACHOQY_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9680829.1"
                     /translation="MSSKNRPRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2666..>2936
                     /locus_tag="ACHOQY_004918"
     CDS             2666..>2936
                     /locus_tag="ACHOQY_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9680830.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQ"
BASE COUNT          688 a          638 c          834 g          776 t
ORIGIN      
        1 atacggcctg tcggctgccg ttctggcgct gattgccgca ggtgcgcctg cgcctgaaat
       61 cctcgaccag tttctggatg aaaaggaagg taaccacacc acggcatacc gtgatggcgc
      121 gggtatctgg accatctgtc gcggtgccac ccgggtggat ggtaagcctg tcctcccggg
      181 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgaacgtg ataaggcgct
      241 ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa ccccagaaag cggggatcgc
      301 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
      361 aattaatgca ggtgatcgca ggggagcgtg tgaggcgatt cgctggtgga ttaaggacgg
      421 tggcagagac tgccgtattc gttcaaacaa ctgctacggt caggtatccc gtcgtgacca
      481 ggagagcgcg ctggcgtgct ggggaatcga cagataagca gaatattttg ctgaaaaata
      541 aggcatggcc acgcgggcgg ataacacgaa atcctgcgaa ctggcgaaac gtaagtgaat
      601 aaaagtaaaa atcccgtttg ttggcagcaa gcggggtttt gtttttatgg cagtaagcta
      661 tgggaggctg ccttgattga ttttagcaaa ctgattaggg agttgcgact catgattagt
      721 caattaccaa actggaaatt tttgctggtc tggagcatcc cttttttatg ggtagtatcc
      781 cagttaattg tggcaattaa ggggtagcta tgtcagacaa actcataacg ccggcaaagg
      841 tcctctgtgt gattgtcggt atttcatttt cactaatgct ggttgctctt tttctgtccc
      901 tcgcctgggt gatgttgtct tcgtcggggc tgctggggtg acagtgactg atgacatcag
      961 cagagcgctg gcttttgcta ttaagtgggt ggctgttggt attgctgtgt ctccgatgct
     1021 atatgggctg gcaaaactgg tcattgcgct gaaatcgtga actttaaaaa gatgagtgct
     1081 gaacttattc gggcaatggc atttgccatt cgtattgtgg ccattgctgt tctggtctgg
     1141 gcaatccgtt ggtggtgaaa tgaaccgcgt tctgtgtgtg gtcatcattg ctctgctggt
     1201 ggcctgtggt gcgcttagtc tggggctgaa tcattaccgc gataacgcca tcacttacaa
     1261 agcgcagcgc gataaaaaag ccagtgaact gaagctggcg aacgcaacca ttactgatat
     1321 gcaggtgcgc cagcgcgatg ttgctgcgct cgatgcaaaa tactcgaggg aattagccga
     1381 tgcgagagct gaaaatgaaa ctctgcgtgc tgatgttgcc gctggtcgta agcgcctgcg
     1441 gatcaacgcc acctgctccg gtaccgtgcg tgaagccacc ggcacctccg gcgtggataa
     1501 tgcaaccggc ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg
     1561 gttgatgaca atgcagaagc agctggaagg ggcgcaggaa tatatccgta ctcagtgcct
     1621 gaaataagtt ttgttgatgc gccgtatcgt cgctgtattc cctcattaac agagaccgca
     1681 gcccgacagg gagactcctc tgcgcgagtg tgcggggata atcaaaaacg atacacaccg
     1741 gggtttaccg cgttaacgga gcgcggcgtt gtcccctcat agtcgccagt ccggtgcgat
     1801 ggtggaagaa acaggacgat gtgttacctc gcaagccctg ttatgtcatg tgtctgattt
     1861 gtgatttaag tcggataatt gtcgttgcca ttaagcagag gattgatgac cgacagggcg
     1921 gcattgttag aataagactt atttttatct gtgcggggaa tgaaaatgaa aagaaatctt
     1981 ccgttaatta ttttgttgtc ttctctggtt atgggctgta cgcaacataa aacagatatg
     2041 ccccgacagt tggttaaggc attaccacaa tatccggcct atgcagcggc aaattatata
     2101 aagggacggg ttgatgtgag gtttgatatt ggtgctgatg gtactgtcac ccgaattgag
     2161 tttattcgtt cagagccgca ccatctgttt gatgagcagg ttgtaaaggc gatggcaaaa
     2221 tggcgatttg agaaggacag gccgtgtaaa ggcgtgaaga aaacgtttat ctttagtcct
     2281 tctgcaccct gattatttca tcagaaatta attatcactc tgttgttatt ctgtacatcc
     2341 cggcagggta agtcttgttc cgtcggatat gaagatgaaa tattgtggga ggacagtggg
     2401 tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg tcatgctggc cgggcgcaga
     2461 tgcgttgcat ctgttgccag ccttctcctg caggcttcaa taacccacgc tgaaaagtta
     2521 ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt ggggaaacgg
     2581 atgttgcggg ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg
     2641 acaagctata tggagaggat tttacatggc tgtgcttcgt acgttaccgg gcagaatcaa
     2701 aacactgaat acccgacggg tgaatattct gaagggtgaa cagcgtcgtg tcagtggcag
     2761 tgcacgtgtt tccctcaagc gtcatatctg gctcagggac gccgggcagt gccgtctctg
     2821 tggtcgtgtg gttgacctct gtgacagtga actcgatcac cgaattgcac ttcagttcgg
     2881 tggtggtaat gaggagacga atctctggac gctctgtacc gaatgccatc gacaaa
//
LOCUS       DAZKGS010000302         2909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKGS010000302 DAZKGS010000000
VERSION     DAZKGS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..245
                     /gene="ariR"
                     /locus_tag="ACHOQY_004919"
                     /gene_synonym="ymgB"
     CDS             <1..245
                     /gene="ariR"
                     /locus_tag="ACHOQY_004919"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP9680831.1"
                     /translation="NAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLSGDNVNNKN
                     IILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            358..606
                     /gene="ymgC"
                     /locus_tag="ACHOQY_004920"
     CDS             358..606
                     /gene="ymgC"
                     /locus_tag="ACHOQY_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP9680832.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            938..2461
                     /locus_tag="ACHOQY_004921"
     CDS             938..2461
                     /locus_tag="ACHOQY_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9680833.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2593..2811
                     /gene="ymgF"
                     /locus_tag="ACHOQY_004922"
     CDS             2593..2811
                     /gene="ymgF"
                     /locus_tag="ACHOQY_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP9680834.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          806 a          582 c          622 g          899 t
ORIGIN      
        1 ataatgcaat aactgataaa gcgttagcaa gttactttcg cagttcgggt aatttgttag
       61 aagaagagtc agcagtgtta gggcaggctg tcaccaattt aatgctttca ggcgataatg
      121 ttaataataa aaatattatc ttaagtctga tacactccct ggaaacaaca agtgatattc
      181 tcaaagctga tgtgattaga aaaacactgg aaatcgtgtt gcgatacaca gctgatgata
      241 tgtaacttct aattattaag tataagttta tagaaaactc attcatctta tttttgtctg
      301 tcgctttaga ctatacacag cataatttta ttgggttaat atttctacga ggctgacatg
      361 aataattcaa tcccagagag atttattttt caatgtgctt tgtttaaaaa tctcgagagg
      421 gaggtgttca tgacgcatgg gtatgttgat agtcatatta ttgatcaggc tttacgcttg
      481 cgtttaaaag atgaaaccag tgtgattctt tctgatctct atctgcaaat attgcagtac
      541 attgaaatgc ataagactac actaacggat atcattatta atgacaggga atccgtgctc
      601 tcttagaact tgagtaaggt tttagaataa acatcagctg tatcaccatg ctgatgcaaa
      661 gtgagcattt caggcgttat gctttcttat tatgtccgca atatcaggtg tcaagaatgg
      721 agagttctcg ctctccattc ttgacgcctg atatcccgcc taacttattt tgtactgcct
      781 acattatgct caataattaa tgggttagat caagatggag aaaattttat cttcggcgtg
      841 gatatttctt atgctctcta ccatgctctc tgtagatgcc agcgcgagcg aatgaatggc
      901 attttcatgt attaaaccct ttggccggga caagcaaatg cgcaatacac tcatacccat
      961 cctcgttgcg atatgcttgt ttattaccgg tgtcgctatt ttgaacatcc agctctggta
     1021 ttccgcaaaa gcagagtacc tggcgggagc gagatatgcc gccaacaata tcaatcatat
     1081 acttgaagaa gcgtcacaag cgactcaaac agcggttaac attgccggga aggaatgcaa
     1141 cctcgaggag caatatcagc ttggcactga agcagctctg aaacctcacc tgcgcacaat
     1201 catcattctc aaacagggaa tagtctggtg tacatccctg cctgggaatc gggtcctgtt
     1261 gtctcgtatt cctgttttcc cggacagtaa tttactgttg gctccagcaa tcgacaccgt
     1321 taatagatta cctatcctgc tctatcagaa ccaatttgca gatacgcgca ttttggttac
     1381 gataagcgat cagcatattc gtggggcact taatgtaccc ttgaaagggg taaggtatgt
     1441 attacgcgtg gcggatgaca ttattggacc aacgggtgat gtgatgacgc ttaatggaca
     1501 ttatccctat accgagaagg ttcactccac aaaatatcat ttcactatta tctttaaccc
     1561 gccgccactc tttagcttct acagacttat cgataaaggc tttgggattt taatatttat
     1621 tctgttaatc gcctgcgccg ctgccttcct gcttgataga tatttcaata aaagcgcaac
     1681 gcctgaagag atcctgcgac gggcaataaa taatggggag atcgtccctt tttaccaacc
     1741 tgtggtaaat ggtcgggaag ggacattgcg gggagttgag gtgttagccc gctggaaaca
     1801 acctcacggt ggatatatat cacccgcggc atttattcca cttgctgaaa aatccggatt
     1861 aatcgttccg cttacgcaaa gcctgatgaa tcaggttgcc agacagatga acgctatcgc
     1921 gagtaaattg ccggaaggtt ttcatattgg aattaatttt agcgcttcgc atattatttc
     1981 gccgacgttt gtcgacgagt gtttaaattt ccgtgacagt tttacccgcc gcgatttaaa
     2041 ccttgttctg gaagtcaccg agcgtgagcc attgaatgtt gatgaaagtc tggttcagcg
     2101 gttgaacatt ctgcatgaaa atggttttgt catcgcgctg gatgatttcg gtactggcta
     2161 ctcagggctt tcttatcttc atgacctgca tattgattat atcaaaattg atcacagttt
     2221 cgttggccgc gtaaacgcag acccagaatc aacccgaatt ctggattgtg tattggatct
     2281 ggcgcgtaaa ctttcgatca gtatcgtcgc tgaaggtgtc gaaacgaatg aacaacttga
     2341 ctatctgaac caaaataata tcacatttca gcagggttat tatttctata aacctgttac
     2401 atacatcgac ctggtcaaaa ttatcctttc taaaccgaag gtgaaggttg tggttgagtg
     2461 aaaattgatc agtaaggcca tagtgcggtg taattataga cagctaatta gctcgttgcc
     2521 tcttgttact attgttcatt attttgtttg ctataattgt ttgaaagttt tgacaggatt
     2581 gccattagta gcatgaacaa tagtaataat ctggattatt tcactctcta tatcatcttt
     2641 tccattgcat ttatgctgat caccctcctg gtcatcctta ttgcaaaacc cagtaccggg
     2701 ctgggagaag tgcttgtgac gataaatttg cttaatgccc ttgtttggct ggcgatcaat
     2761 ctggttaatc gattaaggga aagactcgtc agccacaggg atcagcaata atctttcagt
     2821 ttctcactgt cagtatgcgg ctgaatgggt tgctggcagt gaatgcctgg atcattgaag
     2881 taaaggaatt attgcgcaaa tagttgtca
//
LOCUS       DAZKGS010000303         2883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKGS010000303 DAZKGS010000000
VERSION     DAZKGS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1758
                     /gene="recE"
                     /locus_tag="ACHOQY_004923"
     CDS             <1..1758
                     /gene="recE"
                     /locus_tag="ACHOQY_004923"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFP9680835.1"
                     /translation="YNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVV
                     ASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGK
                     QDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKYQELRAELH
                     EARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQNQPETEKIS
                     PDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQVEAKEKDP
                     EEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVS
                     KSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNR
                     RTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVESAGHAESS
                     IYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGY
                     EAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLADCLNTDE
                     WPAIKTLSLPRWAKEYAND"
     gene            1751..2560
                     /gene="recT"
                     /locus_tag="ACHOQY_004924"
     CDS             1751..2560
                     /gene="recT"
                     /locus_tag="ACHOQY_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFP9680836.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            2617..2811
                     /gene="ralR"
                     /locus_tag="ACHOQY_004925"
     CDS             2617..2811
                     /gene="ralR"
                     /locus_tag="ACHOQY_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFP9680837.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            2780..>2883
                     /locus_tag="ACHOQY_004926"
     CDS             2780..>2883
                     /locus_tag="ACHOQY_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9680838.1"
                     /translation="MEIIMEVFMYKITATIEKEGGTPTNWTRYSKSKLT"
BASE COUNT          884 a          661 c          716 g          622 t
ORIGIN      
        1 tataaccttc atccggcaca cgctaaacgc attgaggaaa ttatcgctga aaataaacca
       61 cccttttctg ttttccgcga caaattcatc accatgcctg gcgggctgga ttattcccgc
      121 gccatcgtgg ttgcgtccgt gaaagaagca ccaattggga tcgaggtcat ccccgcacac
      181 gtcactgaat atctgaacaa agtactgact gaaaccgatc atgccaaccc tgatccggaa
      241 atcgtggata ttgcctgcgg tcgctcctcg gccccgatgc cgcaacgagt aacagaagaa
      301 ggaaaacagg acgatgaaga aaaaccacaa ccatctggcg caatggcaga tgaacaggca
      361 acggctgaaa cagtggaacc gaatgcaact gaacatcatc agaacacgca gccgctggat
      421 gctcagtcac aggtaaattc tgttgatgcg aaatatcagg aactgcgggc agaactccat
      481 gaagcccgga aaaacattcc atcaaaaaat cctgtcgatg ccgataaatt gcttgctgca
      541 tcacgtggtg aatttgttga cggaattagc gacccgaacg atccgaaatg ggttaagggg
      601 atccagactc gcgattctgt gtaccagaac cagccagaaa cggaaaaaat cagcccggat
      661 gcgaaacaac ctgagccagt agtgcaacag gaaccggaaa tagtctgcaa tgcctgcggt
      721 cagactggcg gggataactg ccctgactgt ggtgcggtga tgggcgacgc aacataccag
      781 gaaacattcg gtgaagagaa tcaggttgaa gctaaggaaa aagatccgga ggaaatggaa
      841 ggcgctgaac atccgcacaa tgagaatgct ggcagcgatc cgcatcgcga ttgcagtgat
      901 gaaactggcg aagtcgcaga tcccgtaatc gtagaagaca tagagccagg tatttattac
      961 ggaatttcga atgagaatta ccacgcgggt cccggcgtca gtaagtctca gctcgatgac
     1021 attgctgata ctccggcact gtatttgtgg cgtaaaaatg cccccgtgga cactacaaag
     1081 acaaaaacgc tcgatttagg aaccgctttc cactgccggg tacttgaacc ggaagaattc
     1141 agtaaccgct ttatcgtagc acctgaattt aaccgccgta caaacgccgg aaaagaagaa
     1201 gagaaagcgt ttctgatgga atgcgcaagc acaggaaaaa cggttatcac tgcggaagaa
     1261 ggccggaaaa ttgaactcat gtatcaacgc gttatggctt tgccgctggg gcaatggctt
     1321 gttgaaagcg ccggacacgc tgaatcatca atttactggg aagatccgga aacaggaatt
     1381 ttgtgtcggt gccgtccgga caaaattatt cctgaatttc actggatcat ggacgtgaaa
     1441 accacagcgg atattcaacg attcaaaacg gcttattacg actaccgcta tcacgttcag
     1501 gatgcattct acagtgacgg ttatgaagca cagtttggcg tgctgccaac tttcgttttt
     1561 ctggttgcca gcacaactgt tgaatgcgga cgttacccgg ttgagatttt catgatgggc
     1621 gaagaagcaa aactggcagg ccagcaggaa tatcaccgca atctgaggac cctggctgac
     1681 tgcctgaata ccgatgaatg gccagctatt aaaacgttat cactgccccg ctgggctaag
     1741 gagtatgcaa atgactaagc aaccacctat cgcaaaagcc gatctgcaaa aaacccaggg
     1801 aaaccgtgca ccagcagcag taaaaaataa cgacgtgatc agctttatta atcagccatc
     1861 aatgaaagag caactggcag cagctctccc acgccatatg acggctgaac gaatgatacg
     1921 tatcgccacc acagaaattc gtaaggttcc ggcgctagga aactgtgaca ccatgagttt
     1981 tgtcagtgcg atcgttcagt gttcacagct cggccttgag ccaggtagcg ccctcggcca
     2041 cgcatattta ctgccttttg gtaataaaaa cgaaaagagc ggtaaaaaga acgttcagct
     2101 aatcattggt tatcgcggca tgattgatct ggctcgccgt tctggtcaaa tcgccagcct
     2161 gtcagcccgt gttgtccgtg aaggtgacga gtttagtttt gaatttggcc ttgatgaaaa
     2221 gttaatacac cgcccgggag aaaacgaaga tgcaccagtg acccacgtct atgctgtcgc
     2281 aagactgaaa gacggaggga ctcagtttga agttatgacg cgcaaacaga ttgaactggt
     2341 gcgcagccag agtaaggctg gtaataacgg gccatgggta actcactggg aagaaatggc
     2401 aaaaaaaacg gctattcgtc gcctgtttaa atacctgcct gtctcaattg aaatccagcg
     2461 tgcagtatca atggatgaaa aggaaccact gacaatcgat ccggcagact cctctgtatt
     2521 aaccggggaa tacagtgtaa tcgataattc agaagaataa ttcagcctgg cggtgtaatg
     2581 caccgccaac gtgagacagt ttttatgaca aaaattatga gatatgacga tgttaaacca
     2641 tgtccgtttt gtggttgtcc atctgttaca gtgaaagcaa tttcaggata ttaccgggca
     2701 aaatgcaacg gatgcgaatc ccgaactggc tatggtggaa gtgaaaaaga agcactcgaa
     2761 agatggaata aacgaactac tggaaataat aatggaggtg ttcatgtata aaataactgc
     2821 cactattgaa aaagaaggtg gcactcctac taactggaca agatattcaa aatctaaatt
     2881 aac
//
LOCUS       DAZKGS010000304         2796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKGS010000304 DAZKGS010000000
VERSION     DAZKGS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..321)
                     /gene="bolA"
                     /locus_tag="ACHOQY_004927"
     CDS             complement(4..321)
                     /gene="bolA"
                     /locus_tag="ACHOQY_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9680839.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            626..1204
                     /locus_tag="ACHOQY_004928"
     CDS             626..1204
                     /locus_tag="ACHOQY_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9680840.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            1248..2723
                     /gene="ampG"
                     /locus_tag="ACHOQY_004929"
     CDS             1248..2723
                     /gene="ampG"
                     /locus_tag="ACHOQY_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9680841.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT          600 a          729 c          704 g          763 t
ORIGIN      
        1 tttttacgcg atgcttcctg ctccacgaca gggaggagag gcaaagacgg tgtcctgcaa
       61 cccttcccac tccttaatag tgtaagtatg cagagccagc gcatgaacgg tagtagagag
      121 ttcctccgct aaagtactgt aaatcattcg atgacgattc agaaaacgtt cacccgtaaa
      181 acgatcgctg accagcacaa ctttaaaatg gctttcagag ccggctggga cattgtgacg
      241 atagctttca tccactactt cgaggaatac gggttggaac gccgccctta atttttcttc
      301 tatccgctca cgtatcatca tgaaatatct cctccgacaa cgctgagatg tcatccatcc
      361 ctttaaatac tagccgcttt taccgtttcc attgcagctt aacaacaaat atttaggttt
      421 cgtcctggtt tttccgtcaa gcgtatgaag tttactgatt taaagattga aacacaggcc
      481 tcgcatcagc gtttctttta ccaggggtga ttatttcgtc aacatgatga atttacatgc
      541 cttacccact tcccctcgcc gttggcaatg ttatgatggc gggaattttt accaaacacg
      601 cttaagagtc tctgagagcc cgaacatgtt caaaaaaatc ctcttcccgt tagttgctct
      661 gtttatgctt gcaggatgcg caaaaccgcc aacaactatt gaagtttccc cgacgattac
      721 actgccacag caggatccaa gcctgatggg cgtcaccgta agcattaatg gtgccgatca
      781 gcgtaccgat caggcgctgg caaaagtcac ccgcgataat caaatcgtta ccctgaccgc
      841 ctcccgcgat ctgcgtttcc tgctgcaaga agtgctggaa aaacagatga ccgcgcgtgg
      901 ttacatggtt ggtccgaatg gcccggttaa tctgcaaatc atcgttagcc aactgtatgc
      961 tgacgtgtcc cagggcaacg tgcgctacaa catcgctacc aaagcggata tcgccatcat
     1021 cgctaccgcg cagaatggca acaaaatgac caaaaactat cgtgccagct acaatgttga
     1081 aggtgcgttc caggcctcca acaaaaatat cgccgatgcg gttaacagtg tgctgaccga
     1141 taccatcgct gatatgtctc aggacaccag cattcacgaa ttcatcaagc agaacgcgcg
     1201 ttaatttctg ccctctggcc cggtgcaagc cgggcctgta gacgcccatg tccagtcaat
     1261 atttacgtat ttttcaacag ccgcgttcag ccatattgct gatcctgggt tttgcttccg
     1321 ggctaccgct cgccctgaca tccggcacct tacaggcctg gatgacggtc gagaatattg
     1381 atctcaaaac cattggtttc ttctctctgg taggccaggc ttacgttttt aaattcctct
     1441 ggtcaccgct gatggaccgc tacacgcccc cattttttgg gcggcggcgc ggttggctgc
     1501 tcgccacgca aatcctgtta ttagtcgcca ttgcggcgat gggttttctc gaacctggca
     1561 cccaactccg ctggatggcg gcactggcgg tggtgatcgc tttttgctcg gcctcccagg
     1621 atattgtctt cgatgcgtgg aaaaccgatg tgctcccggc agaagaacgt ggtgcgggcg
     1681 cggcaatcag cgtgctgggt taccgtttag ggatgctggt ttccggcggc ttagccctgt
     1741 ggctggcaga taaatggctg ggctggcagg gcatgtactg gttgatggcg gcgctgttga
     1801 tcccttgtat tatcgcgacg ttgcttgcac cagaaccgac cgacaccatt cctgtgccaa
     1861 aaacgctgga acaagcggtt gttgcacctc tgcgagattt ctttggtcgc aataatgcct
     1921 ggcttatttt gcttcttatc gtgctgtata agctgggcga cgcattcgcc atgagcctga
     1981 caaccacgtt tttgattcgc ggcgtcgggt ttgatgcggg tgaagtaggc gtggttaaca
     2041 aaacgcttgg cttactggcg accattgtgg gcgcattgta cggtgggatt ttgatgcagc
     2101 gcctgtcgct gttccgggcg ctgctgattt tcggcatttt acaaggtgcg tctaacgctg
     2161 gttactggct gctgtcgatt actgataagc atctctacag catgggcgca gccgtctttt
     2221 tcgaaaacct ctgtggcggg atgggcacat cagcctttgt cgcgctgtta atgacgctgt
     2281 gtaataagtc attttccgca actcaatttg ccctgctctc agcgctttcc gctgtaggac
     2341 gagtttatgt cggccccgtg gcgggttggt ttgttgaagc acacggctgg tcgacattct
     2401 atctattctc cgtcgccgct gccgtaccgg ggcttatttt gctgctggtt tgccgccaga
     2461 cgcttgaata tacaggagta aatgacaact ttatctcccg taccgaatat ccggcaggtt
     2521 atgcctttgc catgtggaca ctggcggcgg gcgtcagcct gttggccgtg tggttactgc
     2581 tgttgacgat ggacgcgctg gatttgacgc acttctcttt cctgcctgct ctgctggaag
     2641 tcggggtttt agtcgccctt tctggcgtcg tgcttggtgg tttgctggat tatctggcgc
     2701 tacgaaaaac gcatctgacg taatctgtaa atattattta gctgtagtaa tcactcgccg
     2761 taattattac ggctaaataa acatcagtat cgctta
//
LOCUS       DAZKGS010000305         2753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKGS010000305 DAZKGS010000000
VERSION     DAZKGS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHOQY_004930"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHOQY_004930"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9680842.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHOQY_004931"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHOQY_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9680843.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          698 a          673 c          657 g          725 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt ttcgctaccg
     2701 tgaaagtgct acaaagataa agatttagtt tcaaaaagaa aaaatatgta acc
//
LOCUS       DAZKGS010000306         2747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKGS010000306 DAZKGS010000000
VERSION     DAZKGS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHOQY_004932"
     CDS             <1..480
                     /locus_tag="ACHOQY_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9680844.1"
                     /translation="LNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGNYVGA"
     gene            480..1133
                     /locus_tag="ACHOQY_004933"
     CDS             480..1133
                     /locus_tag="ACHOQY_004933"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9680845.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1130..1456
                     /locus_tag="ACHOQY_004934"
     CDS             1130..1456
                     /locus_tag="ACHOQY_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP9680846.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1453..1842
                     /locus_tag="ACHOQY_004935"
     CDS             1453..1842
                     /locus_tag="ACHOQY_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9680847.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            1862..2671
                     /locus_tag="ACHOQY_004936"
     CDS             1862..2671
                     /locus_tag="ACHOQY_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9680848.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          672 a          670 c          812 g          593 t
ORIGIN      
        1 ttgaatgaag ttcagaaatt cattgaagcc catccgggat gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt cagcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggaaccgga accacaacca gttcgagaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc acgggtgatt ttgtgcctga cccgccaggc tgaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcggtgctt acggaaaagc
      421 agcaagcgcg ctgcatcggg tgaagagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgagtaataa atattgccag gcgctggtgg aactgcggaa caaaccagcc catgaactga
      541 aggaagtggg cgatcagtgg cgcacgccgg acaacatttt ctggggaatt aacaccctgt
      601 ttggcccgtt tgttctggat ctgtttactg acggtgataa cgccaaatgt gccgcgtatt
      661 acacggcgga agataacgcg ctggcgcatg actggtcaga acgtcttgcg gagcttaaag
      721 gtgctgcctt tggtaatccc ccatacagcc gcgccagtca gcatgagggg caatacatca
      781 ccggcatgcg ttacatcatg aaacatgcca gtgccatgcg tgataagggc gggcgctatg
      841 ttttcctgat caaagctgcc accagcgaag tgtggtggcc ggaagatgcg gaccatattg
      901 cttttattcg cgggcgtatt ggttttgaac tgcctgcctg gtttatcccg aaggatgaga
      961 agcaggtgcc gacaggcgct ttcttcgctg gtgctattgc tgttttcgac aagacctgga
     1021 agggaccggc aatcagctac atcgggcgcg atgaacttga ggcatgtggt gaggcctttc
     1081 tggcgcaggt tcgccagcag gcggaaaaac tggtcaggga gatggcggca tgacgacgtt
     1141 aactcaatgc cagcagcagg tgctggatat gctgatttct taccagcaag aacgtggctt
     1201 tccgccaacc aatcaggagg tggcaaccat gctgggatac cgttcggtga atgcagcggt
     1261 ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg gcgtggcccg
     1321 ggggataact cttcataccg cggtgaagga cgacgacagc gaggcggtcg ggattatccg
     1381 cgcactgctt gccggtgagg aaaacgccag gctgcgtgca gcccactggt tacatgagag
     1441 gggcctgaaa gtatgaagct gatcttgcct ttccctccca gcgtgaacac gtactggcga
     1501 caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg gcgcaaattc
     1561 cagagcgcgg cgtgcgcagc aatagttgag cagttacgtc gtctgccgaa accaacgtcg
     1621 gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg cgatctggac
     1681 aactataaca aggcgctgtt tgacgccctg acccatgcgg gtgtgtggga agacgacagt
     1741 caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa ggtcgagatc
     1801 actatcagta agtacgagaa aacggcgggt gcagccgcct gatcaagagg agaaacgaag
     1861 tatgaataat ctgatggtca ttgatggtat tgaagttcgt cgtgatgctt atgggcgtta
     1921 cagcctgaac gatctgcaca gggcagccgg gggagaacaa aaaaaacgcc cgaaatactg
     1981 gctctccaat aagcaaacct gtgaattgat tgaacaactt ttcaccgagg gtggaattcc
     2041 gcctctggaa aagaatcaac cagttagcgt tattaatggc ggaaataacc aggggacgta
     2101 tgtctgcaaa gaactggtgt atgcctatgc aatgtggatc agcccgtcat tccatctgaa
     2161 ggtgatccgt actttcgata tggtaaccag cgcatcggaa aaattatccg ggcaggctgc
     2221 tgacaagatg caggctggcg tgattctgct ggactttatg cgccgggagt taaacctgtc
     2281 taactcttca gtgcttggtg cctgtcagaa actccaggag gctgttggct taccgaatct
     2341 ggcaccgcgc tatgccattg atgctcctgc tgacgcgcct gatggctcaa gccgtcccac
     2401 gctgtcactg agtgcactgc tgaagcagta tggtatccgc ctgacagcta atcaggcata
     2461 tcaccagatg gtgaagctgg ggatcgtcga gcagcgcgaa cgatacagcc gtaccgcgat
     2521 taacaacatc aaaaaattct ggtcgctgac agcgaaaggc tgcatgttcg gcaagaacat
     2581 caccagtccc gcaaatccgc gcgagacgca gccgcatttc ttcgaatccc gattccctga
     2641 gctgttaaag ctgctcgata ccgttcattg aggtgaccgt gagagcacta ctgacccctg
     2701 aaattgcccc gcgtatgggg atcgtattgt tcaggccagg ttcagag
//
LOCUS       DAZKGS010000307         2741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKGS010000307 DAZKGS010000000
VERSION     DAZKGS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            245..979
                     /locus_tag="ACHOQY_004937"
     CDS             245..979
                     /locus_tag="ACHOQY_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFP9680849.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNNADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACGSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQAAELKARGLLS"
     gene            976..1476
                     /locus_tag="ACHOQY_004938"
     CDS             976..1476
                     /locus_tag="ACHOQY_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9680850.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1550..2122
                     /locus_tag="ACHOQY_004939"
     CDS             1550..2122
                     /locus_tag="ACHOQY_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP9680851.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(2450..>2741)
                     /locus_tag="ACHOQY_004940"
     CDS             complement(2450..>2741)
                     /locus_tag="ACHOQY_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680852.1"
                     /translation="GEHIHLILSYSDTHEDGVNNIKMRYIYNNKHQLLSVAQKIDASS
                     YKTQWDRSEKLDEMLNKLALKLPKDSLVINKIREAIPDDYKTIFYPHLKIAC"
BASE COUNT          725 a          657 c          703 g          656 t
ORIGIN      
        1 gtcattactg cgattgttca ccctttaact tactgtatta cttatctttt ttcttatggt
       61 gaacagaggt gaacagtaaa atataaaaaa acaaacagta agccggtttt tcctgcgacc
      121 ttttcctggc ttgccggtct gaggatgagt ctcctgtgtc agggctggca catctgcaat
      181 gcgtcgtgtt gttgtccggt gtacgtcaca attttcttaa cctgaagtga cgaggagccg
      241 gaaaatgtct gacaacacca tccctgaata tctgcaaccc gcgctggcac aactggaaaa
      301 ggccagagcc gcccatcttg agaacgcccg cctgatggat gagaccgtca cggccattga
      361 acgggcagag caggaaaaaa atgcgctggc gcaggccgac ggaaacaacg ctgacgactg
      421 gcgcacggcc tttcgtgcag ccggtggtgt cctgagcgac gagctgaaac agcgccacat
      481 tgagcgcgtg gcacgccggg agctggtaca ggaatatgac aatctggccg tggtgctgaa
      541 tttcgaacgt gaacgcctga aaggggcgtg tggcagcacg gccaccgcct accggaaggc
      601 acatcatcac cttctgagtc tgtatgcaga gcatgagctg gaacacgccc tgaatgaaac
      661 ctgtgaggcg cttgtccggg caatgcatct gagtatcctg gtacaggaaa atccgctcgc
      721 caacaccacc ggccatcagg gctacgtcgc accggaaaag gctgtcatgc agcaggtgaa
      781 atcatcgctg gaacagaaaa ttaaacagat gcaaatcagc ctcaccggcg agccggttct
      841 ccggctgacc ggactgtcag cggcaacact tccgcacatg gattatgagg tggcaggcac
      901 accggcacag cgcaaggtgt ggcaggacaa aatagaccag caggcagcag agcttaaggc
      961 cagagggctg ctgtcatgat ttactgcccg tcgtgtggac atgttgctca cacccgtcgc
     1021 gcacatttca tggacgatgg caccaagata atgattgcac agtgccggaa tatttattgc
     1081 tctgcgacat ttgaagcgag tgaaagcttt ttctctgact gtaaagattc aggaatggaa
     1141 tacatttcag gcaaacagag ataccgcgat tcactgacgt cagcctccgg cagtatgaaa
     1201 cggccgaaaa gaatgcttgt taccggatat tgttgtcgga gatgtaaagg ccttgcactg
     1261 tcaagaacat cgcggcgtct gtctcaggaa gtcaccgagc gtttttatgt gtgcacggat
     1321 ccgggctgtg gtctggtgtt taaaacgctt cagaccatca accgtttcat tgtccgcccg
     1381 gtcacgccgg acgaactggc agaaagcctg catgaaaaac aggaactgcc gccagtacgc
     1441 ttaaaaacac aatcatattc gctgcgtctg gaatgagggc tgccggttaa caccggccgt
     1501 cgccgcacac cgtattttta ttcttcagca tgatgagaaa gagataacga tggaaagcac
     1561 agccttacag caggcctttg acacctgtca gaataacaaa gcagcatggc tgcaacgcaa
     1621 aaatgagctg gcagcggccg aacaggaata tctgcggctt ctgtcaggag aaggcagaaa
     1681 cgtcagtcgc ctggacgaat tacgcaatat tatcgaagtc agaaaatggc aggtgaatca
     1741 ggccgctggt cgttatattc gttcgcatga agccgttcag cacatcagca tccgcgaccg
     1801 gctgaatgat tttatgcagc agcacggcac agcactggcg gcggcactgg caccggagct
     1861 gatgggctac agtgagctga cggccattgc ccgaaactgt gccatacagc gtgccacaga
     1921 tgccctgcgt gaagccctcc tgtcctggct tgcgaagggg gaaaaaatta attattccgc
     1981 acaggatagc gacattttaa cgaccatcgg attcaggcct gacgcggctt cggtggatga
     2041 cagccgtgaa aaattcaccc ctgcgcagaa catgattttt tcgcgtaaaa gtgcgcaact
     2101 ggcatcacgt cagtctgtgt aaaattcccc gaaaatccgc ctgtttttac tgaaaaaagc
     2161 catgcatcga taaggtgcat ggctttgcat gcgttttcct gcctcatttt ctgcagaccg
     2221 cgccattccc ggcgcggcct gagcgtgtca gtgaaactgc attaaaaccg ccccgcaaag
     2281 cgggcgggcg aggcggggaa agcactgcgc gcaagtggtg ttttataatc aaagacagat
     2341 aactgtatta ttatattggt aattaatagt tgtaacgtaa tgtttgttgg gtaaagcatt
     2401 ttgtgtaaaa agccgctact atagcggctt gaatattgga actatatatt tagcaagcga
     2461 tcttcaggtg aggatagaat atcgttttat aatcatcggg aattgcctct ctgattttat
     2521 taattaccaa gctatcttta ggtaatttta aggctaattt atttagcatt tcatcaagtt
     2581 tttcactacg gtcccattgc gttttataac ttgatgcatc tattttttgt gcaacggata
     2641 atagctgatg cttattgtta taaatatatc tcattttaat attgttgaca ccatcttcat
     2701 gagtgtctga ataagacaat ataagatgta tatgctcacc t
//
LOCUS       DAZKGS010000308         2737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKGS010000308 DAZKGS010000000
VERSION     DAZKGS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..492
                     /locus_tag="ACHOQY_004941"
     CDS             <1..492
                     /locus_tag="ACHOQY_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP9680853.1"
                     /translation="FLHPDFNYIYQNYGDDFVTHTAKFSGETVERAIERAIVSKLNIV
                     VEGTFRNAATPLQTLKKLKDAGYQTEVMIKTTSAALSWESTNERYSKDKEAGNIARKV
                     DKNHHDIVTGLLAENARKVFASNLSDKFAVYSREKMIFSSQAATNDDIATLIQNEISG
                     NTQ"
     gene            complement(528..1319)
                     /locus_tag="ACHOQY_004942"
     CDS             complement(528..1319)
                     /locus_tag="ACHOQY_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP9680854.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(1325..2452)
                     /locus_tag="ACHOQY_004943"
     CDS             complement(1325..2452)
                     /locus_tag="ACHOQY_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFP9680855.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT          700 a          756 c          592 g          689 t
ORIGIN      
        1 tttctacacc ctgattttaa ttatatctac caaaattatg gggatgattt tgttacccat
       61 acagcgaaat tttccggcga aacggtcgaa cgtgcaattg agagagcgat agtcagtaaa
      121 ctcaacattg ttgtcgaagg aacatttcgt aatgccgcga cgccgttaca aacattaaaa
      181 aaactcaaag atgcaggcta ccaaacagaa gtcatgataa aaacaacctc agcggcatta
      241 agctgggaaa gcaccaatga acggtatagt aaggacaaag aggcgggaaa tattgcccga
      301 aaagtggata aaaaccacca tgatatcgtc accggtttgc tggcagaaaa tgcccgcaag
      361 gtgtttgctt cgaacctgtc agataagttt gcggtgtatt cgcgtgagaa gatgatattt
      421 agttcacaag cggcaacgaa tgacgatatt gcaacgctta tacagaatga aatcagcggg
      481 aatacacaat aataataaga gctgatggcg tgaaataacg ccgggcttta tgcccggcgc
      541 attaccgtct ggtacattaa gctatccagt tccttaacgt ccccttctct gctatccggt
      601 aacagcggtg gaatttcccc acgcgccagt tccgcacgaa taaacgcatg taacaatgga
      661 cggcgcggac catattccgc ttctcctgcg cgctgtttac gttccagtaa ttcgtctatt
      721 tctgcacgta acggcgcatc aagttcgctt cccgccagta actcagcaaa gcgcattggc
      781 ggtacgcctt tacccgcttc gacccagcgc acggccagca gcggacgcag cacgtaaaag
      841 tattttttca gtcgaacttc gtcgccctgc aaatatccgg ggaagttttt ctgcgccatc
      901 gaatagtaat gccagcgcgc acgcagcgga gaaaaccaca ttgggaccat tgccttaaac
      961 gccgttatcg tttcttcatc ttgttgataa accaccggag aatccagcca ttctatcagc
     1021 gtcgggttcg cgcctttcaa taatcccagc gccttacgcc actcccaacc ggaaacatct
     1081 aactcgtcgt cgatgggcag ctcaataaca tcgcgcggtg actctactcg cagataccat
     1141 tccagcgggt gaacatacaa aaaccgtaca tcgtagtcgc tgtcgggcga ggcaaatccc
     1201 cagccgcggc tccctgattc acaggcgtac agtactttca catcataccg ccgttcaatt
     1261 tcttttaact gctgcgatac cctttctcgc atcgcagcac tgactccatt taatggcatg
     1321 ttactcaccc tttgatatta accaacccat gcagaacctg atccagaccg ccaaatacag
     1381 agatgttatc gatccgttca gccactctct ccagcgtttc cagttctttt aagcgtaacg
     1441 ctaccgggtt gttttccatg actttagcgg tattgagtaa cgaacgcgtc gctgccgttt
     1501 cttcacgtcg gcgaatcaca ttggcctgcg cggatttctc cgcctcaacc agctgcgcaa
     1561 ggatattttt catatcgcca ggcaggacga tatctttcac ccctaacgag gcaatctcca
     1621 taccaaatgg caacatgcgc gatttcacct gttcgctgac aacgtcatca atgacctgtt
     1681 tatcttccag caattcatcc aacgtacgcg tacctaccgc ttcacgcagg gcaaattgta
     1741 attcccgata caaatgatca atcggcttcg ttaactggct aaatgccaat aacacgtcgc
     1801 tgtagcgcca gttagcggcg aggttaatcc gcagattgac cttatctttc gttaagattt
     1861 cttgcccgct gacttccagt acctgcaagc gggtgtcaac cacttcagcg tcaaccagat
     1921 ggttaatttt ccagtatgcg gtcagccccg gcggtaacag cgcctgcgtt tcaccatcga
     1981 ttttcagcac gcctacatgc caggccggaa cctgcaccgt tagtattgca tcgcggcctt
     2041 taactgcccc tttgcgccgt ggttgcaata ccgcattcat cacctccgtt tgtacctgga
     2101 cttgctgcgt attcatccgc accagtgtca aatcatcctc tacacgccag tacaaacggc
     2161 gcgttgatgg cggcaggatc tcctgcaaaa taccgtccat atacaacgcc ccggcttcaa
     2221 tctccgacaa gtctgccacc aggcaatatt tctccaccca atcaggctga aagcggcgca
     2281 aataatccgc cagcacgtcg ggaacttcgc tgccatcgag cgtaatcagc aagacttctg
     2341 gcgtatttaa ccagggtaaa agatgctcgc ctgcatgtag cactttgtag taatcgccgt
     2401 ttctggagag taatgccagc tgatcttttc ttacgcttat ttttttaatc attatctatt
     2461 ccttgcttaa cagccgtcgc ggaagcagac acgaagcgac aggggaattc ccttttgcct
     2521 gttacccggc atctttatcg gcgacgttat cggggatcgc tgactgcgca atccctcact
     2581 ttttatttaa taagttaaaa ccagtattca tttcaggaat gaatgtggga atcgaaccca
     2641 taattggcgt caaaggctga cggaacacgc ccggaaaaca gcctggccgt tcctggtgtg
     2701 ccgacggggt ttgaccccct ttgtaattca gtttcaa
//
LOCUS       DAZKGS010000309         2711 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKGS010000309 DAZKGS010000000
VERSION     DAZKGS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1751)
                     /locus_tag="ACHOQY_004944"
     CDS             complement(<1..1751)
                     /locus_tag="ACHOQY_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9680856.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVTANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREA"
     gene            complement(1815..>2711)
                     /locus_tag="ACHOQY_004945"
     CDS             complement(1815..>2711)
                     /locus_tag="ACHOQY_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9680857.1"
                     /translation="FEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKARE
                     AGEEVFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDG
                     GGLDDLLGMYVTGRDRETREWLGWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRR
                     VGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLG
                     GAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALF
                     NAVSLMSLNPEPKKKAYEVFFI"
BASE COUNT          574 a          900 c          680 g          557 t
ORIGIN      
        1 gcttcacggg acatatccac cgccacaccg ccgtcatcag ccagataaat atccggtgca
       61 ttcaccagta ccagctggtc acccacatac tgggagacaa tcaccggcag cccctggaag
      121 gtcccgccca gcagggtcat gtccggatat tccttctgcc ccagcgcatt tttacgcatg
      181 gacagcgcca gggcattggt gctggacatc agccagaccg caccggtggg ctgcagattt
      241 gccgtcacaa actggccaaa cgcggcctcg gcatccgcat ccgggttacc ggttgatgcc
      301 gtgcccttca catcatgggt gatggacgcc ggggagacat ccgccaccgc cgcttttttc
      361 gggtccacaa agtctgtgtc cagacgcgcc accaccgctt ccgccagcgc attacggacc
      421 agtgcatcag cagccggact ggaaaaacgg atcaattctt ccgtcagtac cgcaatggcc
      481 gacactttcg catgactgaa ggtgatggat tcaaaatcaa acttcgtcag gggtctggcc
      541 ttaccctcac ccacccagcc ggcagcacca ccggacacct gggcatgcac gcggatattg
      601 aacggcacct gacgaagtgc agggatcccg ccctgaccaa atcgcccgat aatggtctgc
      661 ggacgcaggt aatcaataaa gtcctgcgca tattcctggt attcagacag gctgcctgcc
      721 cactgcgggt ccgtggtggt ccctgccccc accgccgatt tcaggacatg atgcagacgg
      781 ctgtcatccg gatactgacg acgggccact tccagggctt cagagcggac acctttagcc
      841 gcggccagtg atttggcaaa gcgggcgaaa ccaatcccct tctccagttt ctgctcaaca
      901 cggatcaccg gcgctgaagc caccgtggcc acattcccgt taccggcctg tttcaccggc
      961 tgtgccgtgg cggccttact ggtttccagt tcacgcagac gcttcaggtg cgcatccacc
     1021 tgacggattt ccgctgcggt gttgtcgtag tgctcttcct cttccacatc cagtgtgcgg
     1081 ccttcctctg cggctttgtt catgatctcc tcaagggagg ctgccagcgc cgcacgcttg
     1141 ttttcaaaac ttttaatctg ttcaccagta ttcattgctg acttttcctt atgaaaagag
     1201 gttattgact gtgccgaagc gccggcagaa gatgcgattt tcaccaccgg tttccggttg
     1261 ccggacgcgg cagaaaacgg gcggtcgaaa tatttaatgg tccggatggt gcattccgca
     1321 ttcgcgggca cggtgacggc agacacctcc atcagctccc agcgcagaaa atgcagtccg
     1381 cctccgtcca gataagtgta ttcatggggc cggaagccca cagaaagccc cctgaccagc
     1441 ccggtcttaa tggcagccca ggcctcatcc agccgggctg ccatctggga gggcatcccc
     1501 ggctccggct tcaccagcat tgccgtgatt tccagccctt ccctgacccg acgcaccgta
     1561 cactggccta cagggcggga atggtcatgc tgccagagaa acgggatcgt actgccaaac
     1621 tccgccccct ccggctccag gatgtcacca tcccgatccg gagaaggcgt tgacgcaatc
     1681 ccggtgatca cccgttcatc ctcactgaag gatttcaccg tcagcaggga acaggcccgt
     1741 ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc gcagcggttc atgatggtta
     1801 cagggtgagc agggttatat gaaaaaaacc tcatacgctt tctttttcgg ttccggattc
     1861 agggacatca gggacaccgc attgaagagc gccatcagcg ggtcaatttt tccccgtccg
     1921 ctggcctgtt tggtgataag aatggcgtta cctttaggct ccacccgggc attaccgaca
     1981 caccaggcca tcaggggctg atcaccgtga atcagcaccc cttcagccag tttgcgctcg
     2041 gtggttttga tggccccgcc cagcttccag ccctggctta tccccaccac actctcatcg
     2101 gggatcccgg cttccgccag tgaatccaga atctgcccca cacctgacgg gtcaataccg
     2161 atatgatcca gtaactcagc ctcatgaata cgacgcacat actccgccac ttccgccgtg
     2221 tcatccccga cccgacggac aatcgtcatg tctccacagg ccacaaaatc ctgaaaccgg
     2281 gatgcctcac tcttccgtct gaccaccgcg gtttcatgca cccaggcatg gccccagccc
     2341 agccattcgc gggtttccct gtcacggcca gtcacgtaca ttcccagcag atcatccagg
     2401 cccccgccgt caatccccac cgtcaccaca tcagcgcgct gcaggatatc gtccaggctg
     2461 acgcgcctgc cctgctgctc ccagaaatcc gcgcccgccc agcggtcaga acgcagggca
     2521 agaccgattt ccacattggc gtgttttgac atgaagccac gaaatacttc ctcaccagcc
     2581 tcccgggctt tacggtactc ccggtacaga aaggcctcat ccaccgaata accgagattc
     2641 gggttaacca tggcgaggtt ttccatcagc aggtgagccc cgctttccac catttcagga
     2701 ggatgctcaa a
//
LOCUS       DAZKGS010000310         2685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKGS010000310 DAZKGS010000000
VERSION     DAZKGS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..742)
                     /gene="zinT"
                     /locus_tag="ACHOQY_004946"
     CDS             complement(92..742)
                     /gene="zinT"
                     /locus_tag="ACHOQY_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP9680858.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(999..1634)
                     /gene="msrQ"
                     /locus_tag="ACHOQY_004947"
     CDS             complement(999..1634)
                     /gene="msrQ"
                     /locus_tag="ACHOQY_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9680859.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(1635..2639)
                     /gene="msrP"
                     /locus_tag="ACHOQY_004948"
     CDS             complement(1635..2639)
                     /gene="msrP"
                     /locus_tag="ACHOQY_004948"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9680860.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
BASE COUNT          728 a          622 c          614 g          721 t
ORIGIN      
        1 tctgagtttg tcttacttat ttgtcttggc aaattattgt tgttactacc aggcactgtt
       61 ctgttaaata actgaagtca gcgcaaaaaa atcaatgaga catcatctcc tcgaccactt
      121 cttcgctact caactgatag ggataatacg ttggccagtt ttccatttca ttcaataaag
      181 actgttggga atcattaccc ataaaaatgt gaaaatgaga tgattttcgt ggtgcaatta
      241 tatgatcgct aaattgtata tatttagggg ctttactttc aggatcttta cattcgaata
      301 agtaacgaac gcctttcttg cctgatttat aggtgagtat tttgtatcca tcgtaatcat
      361 atttacagga tgttgtttca ttatttctat ggaattcgac aatgccgtcc tcaatgccaa
      421 tcatctcgat gtctgtcaca taacctttgc gataataatc tttaatttca gcaaatgttt
      481 tggttttatc tgcatccgct ttcttctgaa agatggggtc aagtttgcca ctttgcagta
      541 aaggataaac ggattgccag actccatccc agtcactgag cgttcggttt tgtacattgg
      601 catcatcaaa aacaccatta gcagcttttt gttcgacctc tgttaagggt ttgccgtgtg
      661 agtgatgacc atgcgaaaag gcaggagcgc taacaataaa gacacctaaa gcaacagcca
      721 gtttatgaag acgaatcgcc aaaacagttt cctccagagt taatgtatat gatgtgtaat
      781 gttatattgt aacatataac aaaatgcaac aactcttagg agacaagaga atagttctct
      841 caggtgtgct tatctatctt attgaaataa aataaattat gtcgatgatt tgaggttgat
      901 gacaaacgaa acataacttg atgtgatctg cgtaggccgg atagggcgct cgcgccgcat
      961 ccggcaggga taacgcgtac tttgtcggca atatgaagtt atacagataa tttgttgtga
     1021 acctgcttgc gtaaccggtt aaaaagggaa agcaacttct tataccgtaa ggctaaaagc
     1081 agtaccgcca gcccagcgta gatgagtggc tgcggtgaaa taatcttcac tgaccacaaa
     1141 taatgtattg gggccaggat cgcgacaaga tagacgaagt tatgcaactg ttgccagtgt
     1201 ttaccgagtt ttcgctgcat cgcctgggtt gaagtgaacg ctaacgcaag caaaattacc
     1261 cagctgataa tacctaacgt taaataaggc cgggtaatta actccttacc taataacgcc
     1321 agattattca cgcccagctc cagcaatgcg taactggtta aatgcagtgt cgcccaggca
     1381 aagcaccata agcctaacag gcggcgagtg cgtatcaata acggctgttt tgcgtagcgt
     1441 gccagaggtg tgattaacaa ggtcgccagc aaaaatttca gtgcagtgcg accagtaaaa
     1501 tgctgaatat ctttcaccgg atcggcaccc aatccaccgt gattaatcgc ccagaccagc
     1561 cagagaaacg gcaacaatcc ggcaagatgc aggcaaactt tcagccatgt cacctgtttt
     1621 gctgtcagac gcatttagaa attctcccgc aaatccaggc cacgatacag cgatgccacc
     1681 tgctcggcgt aaccattaaa cagtagcgtt ggctggcgct gtacatcgag gatgccgcct
     1741 gaaccaataa atcgttcggt agcctgtgac cagcgcgggt gatcaacatg cggattaacg
     1801 ttggcgtaaa aaccgtattc gtcaggcgct gccagattcc aggtggttgg cggacgctcg
     1861 cgggtcagct taatactgac tatcgattta atccctttaa agccatattt ccacggcaca
     1921 atcagtcgca ccggcgcgcc attttgtggc ggtaacgcct tgccataaac acccacggtc
     1981 atcagtgtga gcggatgcat tgcttcgtcg agacgcaatc cttcgacata aggatatttc
     2041 agcccgccac cgataaagcg gtcctgctgg cctggcattt gttccggtgc ataaattgtt
     2101 tcgaaagcga catacttcgc attgctggtg ggttcggcaa gcgccagcaa tttgtgcagc
     2161 ggaaaaccaa tccacggcac caccatcgac catgcttcca cgcagcgcat acgataaata
     2221 cgctcttcca gcggaaagcg acgggttaaa tcatcgtgat cgagggtcaa tggttttgcc
     2281 acttcgccgc tgattttcag tgtccatgga tcggttttca ggctaccagc attagcggcg
     2341 ggatcggctt tatccagccc gaattcatag aagttgttat aaccggagac tttatctgct
     2401 ggcgtcagtg gcaggtcatt ttgccaggcg gcaggcttgc tgaactccaa cggttttccg
     2461 gcgggggcgg gtgggcgatc attccctttg aaccagctaa gcagatcggc atgcgcagcg
     2521 tgaggcaaag aaagtgcagc cgcgctgatg cccagtgctt ttaacacctg ccgacgcgtc
     2581 ataaagaata ccgactcggc cgtaacatct gattctttta aaaattgatt ctttttcata
     2641 agccatcaca cttttttgtc tttagccagc aagagccgtt tactg
//
LOCUS       DAZKGS010000311         2659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKGS010000311 DAZKGS010000000
VERSION     DAZKGS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1009)
                     /locus_tag="ACHOQY_004949"
     CDS             complement(<1..1009)
                     /locus_tag="ACHOQY_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9680861.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTARPEQ
                     SHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAF
                     SAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLP
                     VS"
     gene            complement(1081..1506)
                     /locus_tag="ACHOQY_004950"
     CDS             complement(1081..1506)
                     /locus_tag="ACHOQY_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9680862.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELHD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKILALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(1490..1771)
                     /locus_tag="ACHOQY_004951"
     CDS             complement(1490..1771)
                     /locus_tag="ACHOQY_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP9680863.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            1872..2291
                     /locus_tag="ACHOQY_004952"
     CDS             1872..2291
                     /locus_tag="ACHOQY_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680864.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
BASE COUNT          660 a          704 c          593 g          702 t
ORIGIN      
        1 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
       61 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
      121 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
      181 tgaatgcgga tttcttcggg tttgctccgg cacgcaacgg gtactcccgc cagacctgtt
      241 cgaacacatc cggataatcc actcgtccca cagactgccc ggtgttttcc gggactaccc
      301 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg tgtgattgct
      361 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
      421 aatcggcttc cggtgtcgtg cctgctggct gaccaggatt gacggtctga acatcccctg
      481 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct tgatctgtat
      541 ctttatctgt atctttatct gtatctttat ctgtcgtgac tcgtcgtgac atgtgcgtga
      601 catttcgtga cgcgccgtga caatcgccat tttgttcccg ctttctttcc ctctctcgct
      661 gcgccctctt gcgctctgca ggagattttg cggtttgcga aatattgccg ttgtcctctt
      721 taagcacctg gcgtttttcc catccagtga ttaaatcacc atcaagtacc cgcccctgca
      781 tcgtctgcaa aattgaatca attacctctt ctgtcacgtc gagcgcactt gccaaatctt
      841 ctgtcgtgac atcaatgtga cctcgcgtga catttcgtga cgcgctcacc aggaggtgga
      901 tatacactgc catcactgtt gcaattggct gccctgacac cctggcaatt gttcgccact
      961 tagggtcatt tggcatgtca tgccataatc tgagccaggc gttagccata ctcacctctt
     1021 ctgataccga atctttttac tcacgagttg ccggaagcga ttcgatatgg ctattatcac
     1081 tcaatgcact gccacagcat ttcctgccgg gccaccacgg ttcatctgat tgaaaccggc
     1141 gattgccacc gcgacaaaat catcagcgtc tctcaccagt cgctcccgcg tctccaccag
     1201 ctcccgaaaa taagctgaac tgtggctgcg cattctggcc accagcaaag gtggcattgc
     1261 cttttcgatc gctggtaaca acgccagaat tttttcaact gcatcagggg tgtctttctc
     1321 tacccagcgg aaaattttct gggtattgcg agccagggct tccggatggc tgtcgtcatg
     1381 cagttctggg aacgtcatac ccagttcaaa ataagccctg gttatttcag ctgccggaac
     1441 tttttcaccg tccggatgcg cccaggcatt catcgccatg cggatgtgtt catgcttgat
     1501 tttcatgaat caagctccta gaaagtggtt gtgttaacgt tttggtatct tccagctcgg
     1561 gccaaatatt catccaatca aaaggcctta gttgctgacg tgtaacttca ccattactgg
     1621 ctcgctcaat aaggacacat aacgatgccc ctaacacttg acctttactc aatgcctttc
     1681 ttagataacc gatgctggta ccacactcgc atgcaaacat acgctgttca tctgacgaaa
     1741 gagaattgag aaatattctt aattcttcca tagctactcc ttagtaaaca cagcaaagaa
     1801 tacctacagg taaacaaaag tcaataccca caggttgttt accttgcagt aatcgcatct
     1861 attatttacc tatggacaaa tatgaattta gacgacagca actcatcaaa attcgtgatg
     1921 agaaatgcga tggtaaagcg gttaacgtgg ccagaaagat cgggcgcgag ccttcttatg
     1981 tatcaagaat gttgtaccca gaggggaaaa agggaaaaaa acggatcgct gatgatatgg
     2041 tggagattat cgaagagtcc tttgggttac cccggggatg gatggatggt atcgtttcat
     2101 catcaacgaa cacagcctcc agttatgaaa caagggttct aacgccacga caacgtattt
     2161 ttttagatct cttagacgaa ctgccagaaa gtgaagcgga taacttatta aaaactcttg
     2221 aagagaaaaa acagtattac aatatgatct acgaagaaat ccgtaaaaag aaagcacaaa
     2281 acgcatcata gctcaccaaa caactagtca ccagttaaga caccgcaaaa agttacccat
     2341 gggtatttac tttttaaata cctatgggta tccttctttt cataccaact caccccgctc
     2401 cacagaatgc agggcaatac ttcgagttat caggcagtgg tcaggggtta agtagccagc
     2461 ccgaggcgta agaacatgac ggcagggttc aactttaata actatgcagc aggtttttgt
     2521 tccgctaccc cagcgttaag gggaaacaga ggatttctca gtgggcgaag tcaaacatca
     2581 gaatggaagg catccaggga tcagcaaaga aacagcgatg gcgctttata ttgatatcag
     2641 cgccattgcc ggacaggta
//
LOCUS       DAZKGS010000312         2651 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKGS010000312 DAZKGS010000000
VERSION     DAZKGS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1127)
                     /locus_tag="ACHOQY_004953"
     CDS             complement(<1..1127)
                     /locus_tag="ACHOQY_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspD"
                     /protein_id="HFP9680865.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPVLWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIV"
     gene            complement(1140..1718)
                     /gene="espA"
                     /locus_tag="ACHOQY_004954"
     CDS             complement(1140..1718)
                     /gene="espA"
                     /locus_tag="ACHOQY_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP9680866.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(1776..>2651)
                     /locus_tag="ACHOQY_004955"
     CDS             complement(1776..>2651)
                     /locus_tag="ACHOQY_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFP9680867.1"
                     /translation="EGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKELS
                     LKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDD
                     WELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLK
                     VIIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINKDKDVILN
                     EVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRDNT
                     LLMIGKVIDYKEDVI"
BASE COUNT          744 a          625 c          476 g          806 t
ORIGIN      
        1 acaatacggc tatttactcg tgctgagtcg gatattaact gagaagcatt ttgtaaatag
       61 cttgcctggt ttcgcaactc ggctgcagct ttatccagct caaccttcgc actctcaccc
      121 aggttttcac tctgtcgagt taagttttgt accgcgtaag cagataactg agccaatatg
      181 ctgtattgcg cagaatcatt attcagcaca gttgtcccaa tagaacgtgc agaggtcgta
      241 aatccatttg ccgttgccgc aacttttgaa acaacgttct caacaaatac atcggccagt
      301 ttagtaactg ttttcacgat gtttgaacca actttgttag cgacatcgcc aactttagac
      361 ataagtgaag acaccccgcc aatgccggct gtcagaatac ttgcagcaat agatactccg
      421 ccaaagactt gcgctgctgt ctttaacgcc tgcggggcct tatcgcccat tacatcaact
      481 gcagtttgca gtgccattgc tgttgcgcta acggcaacaa cagcccagag taccgggtta
      541 aaaatagctg caatagcagt taccgcgacg cccaaccaac caaagacctg accaagaatt
      601 ttactttttt gtgctttctc ttcggctttc tgttgttctt cgagctgctt tttaaactcc
      661 agcgttttat tatccagcgc cttattttgc ccatccgtgt agatctcgtt ggagtttttc
      721 atactcgcaa ctttctgcgc tgaagcatcc agggataaca gggtgaccat catcatcatg
      781 gcctgagggt caacgttatt cacctgagaa agataaggat agccagagct tgcattctga
      841 gtgctgcgct gtggttcggt tccctctgtt cgactcctaa cttcaccact aataccacca
      901 aacagtttac tcaggacatc ctcagcaaca gagggcgtca ctaatgagtg gcctgccggt
      961 ggcgttggta aagcggtact ttctgtccaa cttgctgatg gggccgtgga tttaaccagt
     1021 tgtaaatcca gcgcggaagt cacttcaggc tgattaatac ctgaagtagc cgtagcagcg
     1081 ctggccccag acctcataga ctggatatcg ctatttacat taagcatagt tatctccggt
     1141 tatttaccaa gggatattgc tgaaatagtt ctgtattgca gtgactgaat atcagaacgt
     1201 gcactcgtta ataggtttaa cgtatttgac atttgctgta tttcaagctg gctattattc
     1261 actaccgtgg tcaagttatt cgatttggcc gaaatagcgg ccttcaccgt ttgcaaatca
     1321 ccagcgccta attcagcatt tagatcgcta ataccactta ctgtaatgtc attgcgagga
     1381 tcatttatat agtcaatcac ttcttgagga agtttggctt tcttattctt gtcagaacta
     1441 ctctgaacat cagcaatttt agcatccaca agattagcca ttttttgggc tgtggttgac
     1501 tccttagatg cctcattcat atcagcaaac tttgcaatcg acagatcgct ttgtgcctga
     1561 tacatatagg caaacatgag aagcgcagcc tgaaaaacac cgactttatt aaatagctga
     1621 actacttcgt ctttcgacat actgcctaag tcatagactg tcgatgtcga agtactcgcg
     1681 ttcgcactag caactgatgt tgcagttgat gtatccatgt atatacctct taataatctt
     1741 tcttagcaag accaaaaacg attaaaaaac caatatcaaa taacatcctc cttataatca
     1801 attactttac caatcattaa taatgtatta tctctctgtt ccctatcaat aaaacaagcg
     1861 tcaggtaatg atgcaaaaaa gtctttcaat aggtggaaat aataaacatg tttatcttcg
     1921 acaatcgata cccgagaagg gctttccagg agccagtctt ctgttaacca tacatgttca
     1981 ataatagaca atacttcatt taaaatgaca tctttatctt tattaataga gattgcaata
     2041 atatctgtat agttatcaag cgataaaaaa atgattaccc ggcataaatc gctaataatt
     2101 gaagatgttt tcaccttatc ttcaaaagaa gacatataag acagatcgaa cgatagcgcc
     2161 ctaataataa cctttaagtg cttctcacgc tctggaagat ccttaatctc cataaaccag
     2221 tcaagtaaag aggctccctt ttcagaatcc tcatccttgg ccttttcata aagctttttg
     2281 atcttctgga taccttcctg atttaactca cccaccccta accaggaaag cagagccaac
     2341 tcccaatcat cttcgcctaa taaatctaat aataatttgg cgtatttctt ttttaattca
     2401 atcttaacaa acttagattg taataaagaa gatataagtg caacaatctc cccttttggc
     2461 atattaccaa acatagccaa attaggatca tgctgttcca gcagcccact gttctttaat
     2521 gatagttctt ttaatttatc gctatcggtc agagtaagca gctcttgcat atcattaagc
     2581 agcttctccg ttgtcgtacc ttcctcttct ttccttgcac tgcctttgcg atatcccagg
     2641 cttagccctt c
//
LOCUS       DAZKGS010000313         2601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKGS010000313 DAZKGS010000000
VERSION     DAZKGS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..411
                     /locus_tag="ACHOQY_004956"
     CDS             37..411
                     /locus_tag="ACHOQY_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9680868.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            408..1229
                     /locus_tag="ACHOQY_004957"
     CDS             408..1229
                     /locus_tag="ACHOQY_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9680869.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1467..1829
                     /locus_tag="ACHOQY_004958"
                     /pseudo
     CDS             1467..1829
                     /locus_tag="ACHOQY_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448682.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            1826..1993
                     /locus_tag="ACHOQY_004959"
     CDS             1826..1993
                     /locus_tag="ACHOQY_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9680870.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            2400..>2601
                     /locus_tag="ACHOQY_004960"
     CDS             2400..>2601
                     /locus_tag="ACHOQY_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9680871.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPEWYYVI"
BASE COUNT          650 a          529 c          763 g          659 t
ORIGIN      
        1 tttgcaaccg gcgtgctcgg gtaaaagagg ttactgatgc gtatagagtt tgttttgctt
       61 tacccgccga cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      121 gccggtgagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
      181 agcctgtccg gaaggctggc gataaagatt attgccgagc caccggataa gcgccgccgt
      241 gacctggaca atattctgaa agcgccgctg gatgcgctga cgcatgcggg agtgttaatg
      301 gacgatgagc agtttgatga aatcaatatc gttcgtggtc agccagtatc tggtggacgt
      361 ctgggggtga agatttaccc cataatgctt gaagggctgg tcaaaaaatg aaactggaag
      421 atttaccgaa atactactcc ccaaaatccc ccggcctgac tgatgcatcg gcctcaacgt
      481 cgaaagatac gctgagtatc actgatgtga tggccgcgca gggcatgaca cagaattggg
      541 ctgagatggg gttttctgcg ttccttggga aaatgggcat tagtatgaat gacagagagc
      601 gggcaacaga attgctgaca gaatatgcac tcagtcggtg tgatcgcgtg gcggcgttaa
      661 gaaaactccc ggcagaaata aaaccggcag tgatgcgtat tatggcttcg tatgcgtttg
      721 aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc ctgttgtcac ggaaaaaaat
      781 ttattgaaag cgaggttttt acaaacaaga tccagtatcc ggatggtaag ccgccagtgt
      841 gggcaaagtg cacaaaaggc gtgtatccgt cttactggga ggaatggaaa aaagtcaggg
      901 aggtggtaaa agttgcctgt ccggagtgtg gagggaaggg ggaggtttcc accgcctgta
      961 aagattgtcg tgggcgcggt gttgccattc atcgtgaaga gtcggtaaaa cgtggtatgc
     1021 ctgttatcag agactgccag cgttgtggtg gtcgtggcta tgaaagatta ccttcaacgg
     1081 aggcatttaa tgccatatgt aatgtaaccg atgccatatc tcttgataca tggaaaaaaa
     1141 cagttaaacg tttttacgat acgctggtgg tgcagtttga tattgaagaa gcatgggcag
     1201 aacaacaact gaaaaaggtg accagatagc tttgttgatt tttcccgaat ctgtggtaaa
     1261 tttgccctaa cgatgggcgt tttatgcctg acgttagaag attttttaca cccgtcgcca
     1321 ggcgggtttt tttatgactg aaatcacgcc agtacagtaa acgcgctggt ggttgtgaat
     1381 accggtcttt cagcttgctg gctttttcga caagagttat tggtgtgtca cgttaaccgg
     1441 aaaaaggaaa gtttgagaaa cgcgatctgg cacaggcggt tattaatgct gcctacctgg
     1501 tggcctgtgc agatggtgaa tgtgaggctt cctagaaagc gaagatcgaa caggtactgc
     1561 gtaatcagcc tgcgctgtcc gcgtttacgt cagaaattaa tgcgattagc gcaaccatta
     1621 tcggtcagct ggatacgaac tttaaaattg gtcgtcgtgc ggcgttacgt gagatcgagg
     1681 atgtgaaaca cgatacgcgt gaagcggaag atgtgctgga tgtggcggtg gccattgcgg
     1741 aggcagacgg cgaaattgag ccggaagagc gcaaggtgct ggaagagatt gccggtgttc
     1801 tgggtcttcg tctggagaat caccagtgac ggtaaaactg cgcctggctg tggctgcact
     1861 cctgctgttt ctggtggtga tggtggattt caccagcaga atcatgtcgg tgctggcgga
     1921 tggggtgctg gtctgcggca ttgtggtatt gctgtggccg gtgataaaaa gaaacagcct
     1981 gcataatgct tgattttttt gtttactgtt tattaaaaat actactgcat ggtgaatccc
     2041 cctgtgcgga ggggcaatca gcaaccaggt atatgtgata atcgcggatt caggtgctga
     2101 tactgaattc accgggaggc acccggcacc atgcaagaaa aagaatgtgc atgcaaacat
     2161 gcccctctcc ggaggggctt ttttatgggt aaaaaatgcc cgaatgggtt cgggcaatag
     2221 catgagatac tgatattgtt gtgttgttat cgtgtggatt ttaaccaggg tttatcaggc
     2281 tgcgcaactg cgtggccttt tttcatttct tgggctgtag tccccgtgtg tcattcaggc
     2341 ttccggacta cagcccactc catatctgat ttaatacact atcccggccg ggaggaataa
     2401 tgacatttaa acattatgat gttgtcaggg cggcgtcgcc gtcagacctt gcggaaaagc
     2461 tgacacacaa actgaaagag ggctggcagc cgtttgggag tccggtggcc ataacccctt
     2521 ataccctgat gcaggcgatt acagcagaag gtgatgtggt ggtcagtggt gcaactgagc
     2581 cggagtggta ctacgtcatc g
//
LOCUS       DAZKGS010000314         2573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKGS010000314 DAZKGS010000000
VERSION     DAZKGS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..758)
                     /locus_tag="ACHOQY_004961"
     CDS             complement(24..758)
                     /locus_tag="ACHOQY_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP9680872.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(755..1086)
                     /gene="sctI"
                     /locus_tag="ACHOQY_004962"
                     /pseudo
     CDS             complement(755..1086)
                     /gene="sctI"
                     /locus_tag="ACHOQY_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1106..1346)
                     /locus_tag="ACHOQY_004963"
                     /pseudo
     CDS             complement(1106..1346)
                     /locus_tag="ACHOQY_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1360..2541)
                     /locus_tag="ACHOQY_004964"
     CDS             complement(1360..2541)
                     /locus_tag="ACHOQY_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP9680873.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQKPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEVLYGRDHVIYINTNILDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQ
                     E"
BASE COUNT          791 a          504 c          402 g          876 t
ORIGIN      
        1 agattcataa cgctctcata tgtctactta attttcaacc tgactaaatt agttagaatg
       61 gccctatact tccataacag ccagcaagtc gctacggata ttaatgcaag taagatagaa
      121 accggcatag ccttatcata agcaaaaaca ggttcgctaa tttcatatgt tggtgcttgc
      181 tcaataatgt ctcttcgttt tgacaataca acagaaatat tttcatattg tacgcttgca
      241 gagctattaa caataaatct cttgatatca tttattttta tttctgggtt gatatctttt
      301 tcatatactg caagtacaga aatatgaatt ggtaaagcag ttttaccact atcgccatta
      361 tcaacatcgt aactaacatg tactctcgaa gaaataatgc catccataat tttgagagat
      421 tgctctaacc gctgctcaat agcagaatat agccttgctt tttccgctcg tggagatgaa
      481 acgagtgcat ctgcagggaa catctgcgat atttgaatat caggcttacc cggtagattg
      541 tagattttta gccaatccac cgcagaagca aaatccgttg gttcgacaaa tattgaaaat
      601 cctgttttgc cttgatcctt cttttcagca ttaatattat gtctttgtaa aacagcaagg
      661 acatcattag cttgctgttg atcaagatgg ttcaataatt cctgctgctt gcagccgcac
      721 aacagcagga taaacaataa taatgatata tatttcatga gcgggtcaac gtctcaactg
      781 cagcgacaat ctttcttgct aatgttccac caatgaaacc tcgacattat aattaagcac
      841 atgatcctgc aagactaata atttttcagg agttaacccc tctttatcag accttaatgc
      901 ttgtaacgca ttttgctgcc aattttcagt ttgtacagca tagttggcaa aagcggagat
      961 cattttatca tccgggttgt caagattgag aacccggtca tttaaatcaa tcactttcgc
     1021 ttgctgtatt tcatgagcag ttgattgctc agctctatca ataggtggca tattgcttac
     1081 taacataata aaatccttat ctagtttagc ggaagttttg tatgattgct gaatcaagat
     1141 ctttatacgc ttttacaacg ttagattgcg catttcgata taatgtatac tcagctaatg
     1201 cactttgata ttcggcaaga aatttcgtgt cggagggatt ggttggctaa atcctccaac
     1261 gctttttcaa cttgctggtt cagatcatca acgccctgat caaattgatt gctgatatcc
     1321 ataatataac cattccaatc tgccataagt cctcctcaat tattcctgaa atgtccagtt
     1381 gctaccttga gacttctcga atcggttatc tccgtagcta aatgaactat tatttgtgtc
     1441 ctgatttttc aaattcacat taaatcttac atattgttta ccccattgtt tataatattc
     1501 gtcaacatag ttattaattt tatttaattc actgtcactt aacgtaccaa tgatattgaa
     1561 atacacattg ttagctgttt tatatttttc ataagaaaga cctatcgctg acaacgtttc
     1621 aatagcctga ctttccaata cattatcatc taataccgca atacttatat tgctggcata
     1681 tggcatagtt tgcagtaatc ctttaataag attatcgatt agttgctgtg tgatagcgtt
     1741 ccgttgctta ctcactgtca atcttagatt actggcatct aatagttgaa gtttaaaata
     1801 ttgtaaatca gggaaattat cagcaagcca tgaaaaaatt cgtatcgatt catcatcagg
     1861 atttatcacc cgtactggct ttcctggttg atttttttcc agtgcttgtt tgacccagac
     1921 tgcttcatcc aaaatattgg tattaatata gataacatga tctctaccat ataaaacttc
     1981 ataattcctg tttttgttta caagaatttt atcgatatgt ctcagctgcg gatcattact
     2041 ggcattaaca atataaaaag caatgaaaat gatagcaaaa agaaaaaaaa gcattacata
     2101 cttaaacagc ttactgctaa agaaatgttg ttttgtagaa ataatgttat tttcaataat
     2161 gcctggcaat gaatactgcc aaacttcatt tttttctttt acagcaaatt tgagacctaa
     2221 aacttctatt tgctgattca atgttaacgt tctatcttca ggtttttgtg ctgttaattc
     2281 tctgaatgtt actaccagat cagggtcggt agtaattatt atctcaaaac tacctgtaag
     2341 ctcgccataa ggaacgacta ttgtgttttc tggtaactca gaaaaagcat cacttcttcc
     2401 taacgaaaca tcattaccaa tgataacaag tagacgccca ttaagtattt catactcgca
     2461 tccattcaat ggtccgctca acaaacgaat ggcataagat tccaataagt cttgtgataa
     2521 gaatttatca ttattttcca tgaataccac ccgctaattc cataagctct agt
//
LOCUS       DAZKGS010000315         2558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKGS010000315 DAZKGS010000000
VERSION     DAZKGS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..410)
                     /locus_tag="ACHOQY_004965"
     CDS             complement(72..410)
                     /locus_tag="ACHOQY_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9680874.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(413..718)
                     /locus_tag="ACHOQY_004966"
     CDS             complement(413..718)
                     /locus_tag="ACHOQY_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP9680875.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1033..1683)
                     /locus_tag="ACHOQY_004967"
     CDS             complement(1033..1683)
                     /locus_tag="ACHOQY_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9680876.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            1764..1949
                     /locus_tag="ACHOQY_004968"
     CDS             1764..1949
                     /locus_tag="ACHOQY_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9680877.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2059..2355
                     /locus_tag="ACHOQY_004969"
     CDS             2059..2355
                     /locus_tag="ACHOQY_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9680878.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            2388..>2558
                     /locus_tag="ACHOQY_004970"
     CDS             2388..>2558
                     /locus_tag="ACHOQY_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9680879.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAIL"
BASE COUNT          666 a          572 c          538 g          782 t
ORIGIN      
        1 acccatttca ctgcgtggct tgctgtaata aattaggtta gttcagccaa taaaaaaccc
       61 accgaagtgg gctatgacca ttttttattt ggatttcgtt ggtgagcgtg attaacaact
      121 ctgtgcatta catcctcata tttttcatct tcaatttttt cgacatcgcg aggaaatggt
      181 gttgctaatg ctttgtcaac tttgtccatt gggtcttcat taatcttata ttcaggaccg
      241 tcatctatag cattaaatcc aggtgttaca ccgtttttta atgcatatgc tatcctcttt
      301 tcccatctcg ctattctcct cctgtctcga gatgtaagac ctctatcaga tacttttctg
      361 ttttgtccgc ggtcaggatt aacataaata gtctttttca ccataagcat actcaataag
      421 caccgtacgg tagtttactg tacaatttta ttttttggac tgcatgtatt ttgtttccta
      481 atgggtttga atccttgtaa taaatacttc tattttttcg aacaacttct tctttcttct
      541 tgcagcaaag gcttcctagt gatgctgctt tgtctgctct gacgcaacca gagagcttta
      601 gcgcaatttt tcgcgccagt gcttcattac tgcgtcgctc ggcaataagt tctgctctgc
      661 gagctttgta gcggcttttt gccgtacctt tggattcttt ccagacaatg gttaccatga
      721 tggtctcctt taagtggctt tggcgcatga cgcgtcgagg tgcttatctt ctcgatcgct
      781 gtcttgcagc tgcaattcgc gccatcccca aaaccactca agttctggtc tcaacggtta
      841 ggttgagagt tcgtcgatgt taaagagcct gccaatctgt tccgtttggc ttccagcctc
      901 ctgctgacgg cttaaaatta agacctctta attttttggt taagtgcatt tttgaagaaa
      961 acttaatttt atgggcgcga atttagtttg tctttgattt ttaacgggaa ataaaaaagg
     1021 ggcgaaagcc ccttaaggaa ggtttgctag cttggcatca acgacaacgc caatgatttt
     1081 acagttccca ttgatttcaa tcattgggta ttgtggattg agtggtttca ggaattttct
     1141 accggcatca ataactaact ttttgaatgt cgcctcgttt tctccttcaa gtttggcgac
     1201 taccagcttt ccattacgtg gttcgacttc tgggtcgacg agaataatca tcccttcagg
     1261 aatactcagt cctgccgggg cagtcattga atcaccttta acgtcgagcc aaaaagagtc
     1321 ttcagaacaa tctaccgttg tgtcgtacca gttatctatt gcacgcctat gatatggctc
     1381 tacagcttcc atccaacatc ctgcgcttac ccaactaatt agaggatacg aacctcttgg
     1441 atcatgcctg ctgtgatagg caatgtttga aagactatcc tctcctttca acaggtaatc
     1501 aggggagcac tgcaaagcct tggctaaggc caataggttt tcgccattgg gctcagtttc
     1561 agatcgctcc cattgggaaa tagcaacatt agacacgcca accatcttgc caagggcagc
     1621 ctgcctaatc ttgagttctt ttctgcgagc gcgaatacgc tcacccatca gttgtgtatt
     1681 catagttaag acatcttaaa taaacttgac ttaagattcc tttggtggat aatttaagtg
     1741 ttctttaatt tcggagcgag tctatgtaca aaaaagatgt tattgaccac ttcggaaccc
     1801 agcgtgctgt tgctaaagca ctaggcatta gcgatgcagc agtctctcag tggaaagaag
     1861 ttatcccaga gaaagacgcc tatcgattgg aaatcgttac agctggcgcc ctgaagtatc
     1921 aagaaagtgc ttaccgccaa gcggcataag taaattgctc tttaacagtt ctggcctttc
     1981 acctctaacc gggtgagcaa acatcagcgg caaatccact gggtgtgccg ctatatatca
     2041 atataggaaa attaacaaat ggcacaagca agttacagca agctaacaca gcgagaaatt
     2101 gatcgcgcag aaacagattt actcatcaac ctgtcaacgc ttacccagcg cggtctggca
     2161 aagatgattg gctgtcatga atcgaagata agcagaacgg actggagatt tattgcttcg
     2221 gtcttgtgtg ctttcggaat ggcatcagac atcagtccga ttagcagggc ttttaagtat
     2281 gcgcttgatg aaatcacaaa gaaaaaatcc ccggtggccg ccggggactc taagcaaatt
     2341 gatatgcaat tctgagggaa ttactggatc aatccacagg agtcattatg acaaatacag
     2401 caaaaatact caacttcggc agaggtaact ttgccgaaca ggagcgtaat gtggcagatc
     2461 tcgatgatgg ttacgccaga ctatcaaata tgctgattga ggcttattca ggcgcagatc
     2521 tgaccaagcg acagtttaaa gtgctgcttg ccattctg
//
LOCUS       DAZKGS010000316         2532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKGS010000316 DAZKGS010000000
VERSION     DAZKGS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..665
                     /gene="tnpA"
                     /locus_tag="ACHOQY_004971"
     CDS             15..665
                     /gene="tnpA"
                     /locus_tag="ACHOQY_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9680880.1"
                     /translation="MPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTIHTLFQRFI
                     ASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSREFKVRLAKQ
                     ALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRP
                     VTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIREMKGGTR"
     gene            662..1009
                     /gene="tnpB"
                     /locus_tag="ACHOQY_004972"
     CDS             662..1009
                     /gene="tnpB"
                     /locus_tag="ACHOQY_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9680881.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1029..2532
                     /locus_tag="ACHOQY_004973"
                     /pseudo
     CDS             1029..2532
                     /locus_tag="ACHOQY_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          640 a          686 c          707 g          499 t
ORIGIN      
        1 agacaaaaaa agatattccc tgcttccgtt cttatttgcc tgatgctctg cgtttaagat
       61 ttgaagataa actgaccatc cgggccatcg ctcagcgtct aggtctcagt cattccacaa
      121 tacatacgct ttttcagcga tttattgcat ccggtatcgc atggccattg cccgattcag
      181 tttcattagc tcagcttgat gccatccttt atgccaacag aaagaaggaa ttaacagagc
      241 ctcaaatcag cgaaggcaca tggcgaaaag aacggcgagc cagctacagc cgtgaattta
      301 aggtccgtct ggctaagcag gcattacagc ccggtgctgt tgttgcccgg atcgccagag
      361 agcacggtat caatgataac ctgctgttta aatggaaaag ccagtacgag gacggcttac
      421 tgagcgatga tgacatacag gaatgcatgc ctgtcccggt ggctctgact gatacgccag
      481 agccgaccag accagttaca aatcccttct ggcgtaacaa gcctgatgag tgccctgaga
      541 gtgatcccgg aaacgtccca cggtgcgagc tgcatcttaa atcaggtgtg gtaaaactgt
      601 ttgaccctct cactccggaa atgttacggg cgctaatccg cgaaatgaaa ggaggtaccc
      661 gatgataacg ctgccaaccg gtaccagaat ctggatcatc gctggcatca cagatatgcg
      721 ttgtggcttc aacggcctgg cttcgaaggt gcagaacacg ctgaaagatg acccgttctc
      781 cgggcatatc ttcgtcttcc ggggccgcag tggcaaaatg gtgaaaatac tgtgggccga
      841 tcgtgacggg ttatgcctgt tcgccaaacg cctggaacgg ggccgcttcg tctggccggt
      901 aacccgggaa gggaaagtgc acctgacgcc agctcagtta tccatgctac tggaggggat
      961 cgcgtggcaa catcccaaac ggacagaacg gcctggcatc cggatataac ccgtgataaa
     1021 acaggggaat gaacaacaca ctccccgacg acatcgagca actgaaggcc ctgctgatcg
     1081 cacagcaggc tgttatcgtc tgtctggtga aataaccggc tatgcccgcg agatcagctc
     1141 actcagagcg ctggtcgcta aactgcagag aatgttgttc ggtcgcagca gcgagaaaag
     1201 ccgcgagaag atagaaaaga agatcgcacg ggcagaaacg cgtataaccg agctccagaa
     1261 caggcttggt gaggcgcagt tgcaactcac ctcaatggcc ggagagacag cgccgaaaac
     1321 atcagactct cccgtccgca aagcacttcc ggcaacactt ccccgtgaca ggcaggttat
     1381 ctccccggca gaaaccgaat gccccgtctg cagcggcaaa ctgaaaccgc tgggagaaag
     1441 catctctgaa caactggata tcatcaacac cgcgttcagg gtaatcgaaa cggttcgccc
     1501 aaaactggcc tgcagccggt gcgactgtat agttcaggct ccgcagccac caaaacccat
     1561 cgagcgcagt tacgccagtc cggctctgct ggcccgcata atcatggcta agttcgccga
     1621 gcatctgccg ctgtaccgtc agtcggaaat ctatgcccgc cagggcgtgg agctgcaccg
     1681 caatacgatg gggcgctggg ttgacatcat gggagagcag cttcgcccgc tgtatgatga
     1741 actgaagcac tatgtgctga tggcgggtaa agtgcatgcc gatgacacgc cggtaaatgt
     1801 actggagccg ggtcagggta aaacccgtac cggacggctg tgggtctatg ttcgtgacga
     1861 tcgcaacgcc ggttcgacca tgccggcagc ggtgtggttc tcatactctc ccgaccgcaa
     1921 aggcatccac ccacagcaac atctggcgga ctacagaggt atcctgcagg ccgatgcata
     1981 tgcgggttac aatgctcttt acgaaagcgg tcaggtaacc gaagcggctt gtatggcaca
     2041 tgcccgacgc aagatccacg atgtacatgt ccgccatcca acgacagtaa cgggagaagc
     2101 gctccgtcgt atcggggaac tgtacgctat cgaggctgag atccgcggca gtccggcaga
     2161 agagcgactg gcggtcagaa aagccagaac ggtaccgcta atgcagtcgt tgtatgagtg
     2221 gctccagggg cagatgagca cgctgtcgcg ccactcggat acagcgaaag cgttcaccta
     2281 tctgctgaag caatgggacg ctctgaacga atactgccgc aatggctggg tggagatcga
     2341 caataacctg tgtgaaaacg ccctccgggt agttgcactg gggcggcgta actacatgtt
     2401 cttcggctct gatggtggag gcgagagtgc ggcagtgatg tacagcctga tcggtagctg
     2461 caaacttaac ggaatcgagc cggaaacgtg gctacgccac gtgatcagtg taatcaacac
     2521 ctggcctgcc aa
//
LOCUS       DAZKGS010000317         2493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKGS010000317 DAZKGS010000000
VERSION     DAZKGS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            584..811
                     /gene="ykgL"
                     /locus_tag="ACHOQY_004974"
     CDS             584..811
                     /gene="ykgL"
                     /locus_tag="ACHOQY_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFP9680882.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(846..986)
                     /gene="ykgO"
                     /locus_tag="ACHOQY_004975"
     CDS             complement(846..986)
                     /gene="ykgO"
                     /locus_tag="ACHOQY_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9680883.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(986..1249)
                     /gene="ykgM"
                     /locus_tag="ACHOQY_004976"
     CDS             complement(986..1249)
                     /gene="ykgM"
                     /locus_tag="ACHOQY_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9680884.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1613..1714)
                     /gene="ykgR"
                     /locus_tag="ACHOQY_004977"
     CDS             complement(1613..1714)
                     /gene="ykgR"
                     /locus_tag="ACHOQY_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP9680885.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2188..2277)
                     /locus_tag="ACHOQY_004978"
                     /pseudo
     CDS             complement(2188..2277)
                     /locus_tag="ACHOQY_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          740 a          457 c          542 g          754 t
ORIGIN      
        1 tcaatattta gaaaaactga aaatattgct tttttgataa gaagaaaaag acatttggtt
       61 tcataaggtt attggcaata taacgttcaa ttcttacttg cattttatat tggtcaggaa
      121 ctttgccctg aagtgttagg aatagtctta atgcgctgtg tgatggacta gtaaaaaaac
      181 aagttttaag ggtaagggat agaatataaa actataatga gctgtcattg aagatgattt
      241 gccaatcagt aagaaatttt aatggattaa catgccgcac aaaatggtta taggtgttca
      301 ttacaataag tgatggaaaa ttgaacgggc gtatgtacgt catctgttcg gtaagccccc
      361 aagagccaga ttaagtgaag ttaaacgaag gctgccagct gtcacaggaa cgatggcctc
      421 tttgtcaggg cactgagtgc agttatctgt gcgcgttact ctaactggca ctcgaacgtc
      481 ggctacgggt taccggtgga tcagactgtt cctgtggcgt aaacgttttt atgttagtac
      541 caacagattt accacattta tcatcttaac agtttgagtg aaaatgcagg ctgcgccgca
      601 aagggaggag actgagtggc gtgttcagtc taaaagaggg ttgatgccag catacagggg
      661 cgaagctggt caacaagtca acattaagat aatggaatat tcggagagaa acgtcaggca
      721 gttggcctca aatgaacagg aagaatatat ccccaggaaa attaatgttg gtgttataaa
      781 cacaccaaca ttaatccgat ctgattatta atcagacaaa tatcgttaat tcgtcgaatt
      841 ttgaatcaac gttttttctt acgaccctga acggccttaa aacgtggatt agatttacaa
      901 atcacataca accgtccttt tcgcttcaca atctgacagt ctggatggcg ttcttttgcg
      961 gtacgcagag agttaagaac tttcatcacg cccccttttt cgtgctaaca aaacgaccaa
     1021 aacgttgggt gaatcgtgca acatttcctt ctgatgtcac tgttctcagc ttccctgtat
     1081 agaacgggtg cgatttagaa gagacatcaa ttgtcacgta cggatacgtt acgccatcca
     1141 gctcaatctc acggtctgtt ttgatagtcg agccgatttt aaagtactca tcaacactgg
     1201 tgtcgtggaa caccacagta cgatactcag gatggatatt gggcttcatc atttttacct
     1261 gttatgttat aacataaccg taaagataca tgctcatccg gatcatttca acacccaatc
     1321 cctgtgccca tttgtggtat gtggaatttc cccaacgggg aagtctggca ttgttagcag
     1381 ccgttggcga cgctcccata aacgcctgga tgagtgattg atgatggttt tgctgagagt
     1441 atggattttt cgagaatgga ggttatctcc actttactgg ccgatgattt atcgcagtat
     1501 gattgattac ctgacagcgt gtattcctct cccacggata taataattac ttacaaaaag
     1561 ggagaggatg catattttaa atatcactga agtgaacagt ttattgccgt tattaataga
     1621 aatggagaaa taaataggcg tattctacaa ttgcgacaaa aacaacgata ttaatcagtt
     1681 tatgactgat ttgttgtact ttattctctt tcattggtac ttcctcgctt taaaaaagag
     1741 tgcacttcgt aagtgccctt atataaataa cgagtttggt caaccaattt tttgacatgt
     1801 atcacaaatt tgaatagatg tattacatca gctatctttt attgcaccaa cgtcattgat
     1861 atatgtcgac tgaagtcagt tccgggaatg agtctgatct caagactggc ccagtccggg
     1921 cgttgattgg cgctgaggag catacctcat ttcatcataa tgtcgtatct cctggggtgt
     1981 tatacaagat atcgttgttg gtaacctggg aaaggaattg agttctatta aaccgtcaac
     2041 tatgccggat acatactgga ttacactgca ggcacgcctt atgagagaac gtgccgcagt
     2101 gacgggttaa ttatctgaaa gaatttgtga ggctgtatcg gttactcatt gatttgatag
     2161 ttttactctc gggagaataa tagatattta atccattaac ggaaaccagc cagttccttt
     2221 cgatgcctga atttgatccc atagtttatc cggaatggtt aattcatgaa caagttgtgt
     2281 tatcgttcat gagaagcata acgtaaaggg aaaagctcga ttagacggca gaatttgtca
     2341 ggggttatga acgaaattca taaatctgtt tgagtgttgc gatgggtagt gcaagttcga
     2401 tatctccgca atttacagtc cgatgaagga aaatgaatat ccataaaaaa tatattggtt
     2461 tatcctggca tatataccta tttcgacgta ttt
//
LOCUS       DAZKGS010000318         2457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKGS010000318 DAZKGS010000000
VERSION     DAZKGS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..129)
                     /locus_tag="ACHOQY_004979"
     CDS             complement(<1..129)
                     /locus_tag="ACHOQY_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680886.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVI"
     gene            complement(137..>2457)
                     /gene="pdeI"
                     /locus_tag="ACHOQY_004980"
     CDS             complement(137..>2457)
                     /gene="pdeI"
                     /locus_tag="ACHOQY_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9680887.1"
                     /translation="RLIILFLLAALSFIGLFFIINYQLVLERAVKRADSRFELIQKNV
                     GYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSR
                     RANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRP
                     GKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAK
                     NQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKL
                     FYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAK
                     EIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLC
                     GLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKI
                     AMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDD
                     AKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIIS
                     PLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHF
                     HERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLS
                     NLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARE
                     LIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT          724 a          566 c          461 g          706 t
ORIGIN      
        1 aataacggac tgtttcaggc tatacacccg ttcctttacc atttgagtgt agatcccgca
       61 ggcaactgct gaaatcatga taaaaatcag cagactgact gtaagtacga acttattgat
      121 tctggacata cgtaaactac tcttttacta attttccact tttatcccag gcggagaatg
      181 gcatgggttt ctggtacaga tacccctgag cctgaacaca tcctaattta attaattcgc
      241 gcgcaacgtc ttcgctctct acgccttccg caatcaccgg gatccggtta tagcgtgcca
      301 gatcgaaaat aatgcgcaca atttttcccg cagtaccgaa aatatcgccg gtaaattgcg
      361 agtccacctt tatgctgtct ggattaattt cataaaaacg tttcaggttt gacaaaccgg
      421 tgccaaagtc atcaatcgat atccgtaccc cttgtttacg cagttgttca atattgagat
      481 aaaaatgttc attaattcgc tcgatcacgg tttcagtgat ctcaacacag aggctgttcg
      541 ccttcaggcc attttctttc atgatggtgg taaaacgctc atgaaagtgc ggttcgttta
      601 actgccaggg cgaaacatta atatgcagta agaaatcatc cttcagcgac gcacgctgac
      661 taaaatgtct gaattcaccc attgccgttt taataatctg ataacccagc tcattgataa
      721 acccgatatc ttcagcaatg gtaatgaact tcagcggtga aatgatcccc cgctccgcag
      781 aaacccaacg gcacaacgcc tccgcgccca gaatggcgcg ggtgttaata tcaacgatcg
      841 gttgatagac gaggaatagt tcgccaattt ccattgcatg gcgaatatta cgcgcaatcc
      901 agatattctc ttctatttcg ttgagtttcg cgtcatcaca gaaaataaac tcggagaaca
      961 tatggtcacg gatactggaa atcgccagtt tcagtttttc tgcgtaactc caggcgtttt
     1021 ccccctgcaa aggttcacaa atggcagact tatggatgcg cagattgcgg tagccaaagc
     1081 tgctgtcaaa atcgtttacc atcgctattt tacgggtaaa tgtctgtaca ttttcccgac
     1141 acagaagtaa atagagatcg gcattgaatg tcacgatatc gcagcattgc gcatatttct
     1201 cggcaattcg ctgacacaat ttcctgacga ttttattgcg ttcatcaaca ccaaacaccg
     1261 cttccacggc ttccagacca cacagtttga tgatccccac agccagatac tgattattgt
     1321 tggactcaat aatcgccata tttttaataa aaccgctttc ctgatcgtaa gagatcagtg
     1381 agagcaattt atttgaccgt tcaatttcgg catctttcat ttccgcgatc gaatttttga
     1441 tgctgataat ctctttcgca tcgccttcgc gaattttcag agcctcgatc tgatccgttg
     1501 agtcgggcaa aaacgccagc gtattaacca gctcggtcat ttcagaaaat tgccgacgga
     1561 acacacgagt ggtcatgaaa tataccacca gcaacacgat aataatgaac gcaaacggta
     1621 gataaaaaag tttatctgat gcgttcaata aattaccgcc aggataataa aaatagattt
     1681 cgaacaatgc attatttggc actgctgatg tctttgcaat atactccgta tcgacagaat
     1741 caataatatt ataattacct tcgctattat aaataatcag tttttcacga gcgagttgat
     1801 tcttggcaat gatctcatga ccttgtttca aaaatagaac ttcatcatta gcgctgattt
     1861 gatcaagata ctcgttcaga taccgccagt ttaaatcgac atgaatttta tctaccgcta
     1921 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
     1981 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
     2041 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
     2101 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgtgaaaaga
     2161 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
     2221 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
     2281 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
     2341 gttcaaagcg gctatcggca cgttttaccg cgcgttccaa taccagttga tagttaatga
     2401 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agcctta
//
LOCUS       DAZKGS010000319         2455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKGS010000319 DAZKGS010000000
VERSION     DAZKGS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..788)
                     /locus_tag="ACHOQY_004981"
     CDS             complement(<1..788)
                     /locus_tag="ACHOQY_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680888.1"
                     /translation="MEMVVSDGEERFTELDFYYGSKALEGTSEIVTVLTNTILNREVV
                     TKIPSIEGITASFRTSYEGSFRQRFMLKITGEEQRRNYGAIGEGAFLDVMSYYLTRPL
                     LTPYNFTYKRAARIVKELESDYSSLMKRLYGPLLKLHKPIEEQNYTVELKRRRTNLAR
                     YDHTTLENLIVERADDERIIIEASITRFNRLTGTGRLILEEDSESVSFEPSYLWRDFP
                     ADQKRWLSRNLDVNTDNGDFTRVRLEVTSIRNFLGVIKKYSLHRV"
     gene            1587..2060
                     /locus_tag="ACHOQY_004982"
     CDS             1587..2060
                     /locus_tag="ACHOQY_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680889.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
BASE COUNT          686 a          533 c          491 g          745 t
ORIGIN      
        1 actcggtgaa gactatattt tttaatgaca ccaagaaagt ttctgattga tgtcacctct
       61 agccgtacac gagtaaaatc accattatct gtgttgacgt ctaagtttct agatagccag
      121 cgtttttgat ctgctggaaa atctctccaa agatatgaag gttcaaaact tacagactca
      181 gaatcttcct caagaatgag ccttcccgtc cccgttagcc gattaaatct tgtaatagaa
      241 gcttcaataa tgatcctttc atcatcggca cgttcaacaa ttagattttc aagggtcgtg
      301 tgatcatacc gagcaagatt tgtccttctc cgtttcagct caacagtata attttgttct
      361 tctatgggct tatgaagctt caatagtggg ccataaagcc tcttcattaa tgaactataa
      421 tctgactcaa gttctttaac aattctagca gcccttttat atgtgaaatt ataaggtgtt
      481 aataatggtc ttgtaagata ataagacata acatctagaa atgccccctc tccgattgcg
      541 ccatagtttc tacgttgttc ctcacctgtg atcttgagca tgaaacgttg tctaaacgac
      601 ccttcataac tggtacgaaa agatgcggtt atgccttcga ttgatgggat tttagtaact
      661 acttcgcgat ttagaatcgt attagtgagg actgtaacta tctctgacgt gccttcaagt
      721 gcttttgacc catagtaaaa atcaagctct gtgaatcttt cttctccatc acttactacc
      781 atttccatgg gaacatgaat ttccattctc taattccaaa taaaaaccat gggtttaata
      841 tgtcctaatt gatttggttt tggcaactat ttttgattgt ttttcaagaa taattcgaaa
      901 gctattaatt aatggctgga actaataggt tccagttttc aactaatcca gtgacctgct
      961 ccccgctgat caactcagac tgttgttagc aacttacgct cctggtacgg agcgggcatt
     1021 gattacccgc tccgttcacg ttccgcctgg aagttctgca atttatgtct atcggctatt
     1081 gaaaaccttc cggtccttcc caatatcgaa actggcacct ttgccagata acaaagagat
     1141 acgggaacat gatgtacgtc tacagaagac ctccaaatca gctcctttcc atttctcgtt
     1201 tgaagcagta tttgttgcat gaggtggaat caaatcaaca catcatgaga cagtgggaat
     1261 ctgccagaac ggcacttcga aacatccgta acaaagggca tgatccagac accgcctcct
     1321 gggtttcact taaaaatgat ccccgagtct tcacgaatgg cgcagatgaa aagcatgggt
     1381 tcatttttgt tgacgacgac gatcaatcag tccttgttgt ggcctcctgg gtgatagatg
     1441 agtacggttc ccccctcact acatattaat ccctgtacct ggacagaaga gttttctgtc
     1501 cacaactacc aatagcttta cataatgtgt gcatctaact tcaataagtt acttcattac
     1561 caataatgtc taaaaaggaa acgcgcatgg atattgatac gctgggtggc caggaacttc
     1621 gggataagat ttttgctggg ctcaaaatat acgagggtaa acccttcatc gaacggttcg
     1681 gtctgtttat gggcaaggca cagttgctag agtttggcct aaaaaaaatt cttgtttctt
     1741 ttccgggata caaccttgat gaagaaaaat tagagaggct gacattaggc cagacccgcg
     1801 ttgagcttaa aaagctgggg ctgcgcacgg actataatgc ttacctgaag tcattcaaag
     1861 atcagcgcaa tacgatggct cacgagttcc ttgctaattt tgcggtcaca cagcagcttt
     1921 tagacggagc tgcacttatt cgcacatttg agcgtgagtt agaccatgcc tgttacgcgg
     1981 tggagcagtt gatcatcctt ttcgacttca tcaacggtgc cggtgacgta accgcctggc
     2041 ttgagcctac tgccccctga tacttcgtat gcttttatct ctaggctgga cagaatagta
     2101 attctgtcca ggtatgcaga atggctgtct atatatcttc attagttcac agtaggttac
     2161 cggcggcttt gagcgaacag cggaacttcg ccctttaata aatgataata aattttcatt
     2221 atcactttag ttgaaaatgg tgtcgaagat gaagagaaat gtcatattag cctacggagt
     2281 acttgtgaaa taaattcata gcattcccta acatctaaac ttagggctca ctgaacgatg
     2341 gtgaacccta tgccattggg ttaataacta ttgataattt tgtgaagtgc agggttaatt
     2401 gtcgatgaaa tatcaaaatc ggaatttcta atcgacccag tgctccatcc ctctt
//
LOCUS       DAZKGS010000320         2451 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKGS010000320 DAZKGS010000000
VERSION     DAZKGS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..937
                     /locus_tag="ACHOQY_004983"
     CDS             <1..937
                     /locus_tag="ACHOQY_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9680890.1"
                     /translation="FNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDF
                     LNGDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQV
                     YIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQII
                     NVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKV
                     GNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKE
                     QVNGDSLLINWNCAASKSPPKPPDEKQDTPDMREY"
     gene            1005..>2451
                     /gene="rhsD"
                     /locus_tag="ACHOQY_004984"
     CDS             1005..>2451
                     /gene="rhsD"
                     /locus_tag="ACHOQY_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFP9680891.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDT
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVV"
BASE COUNT          542 a          662 c          811 g          436 t
ORIGIN      
        1 gttcaactgg gaccgttata acccggcaga ccaagacagt tcgtgctgga tccgtgtggc
       61 acaggcgtgg gcaggcaccg gttttggcca cctggcgata ccgcgtgtgg gtcaggaggt
      121 gattgtggac ttcctcaacg gcgacccgga ccagccgatt attatggggc gtacctacca
      181 ccatgaaaac cgcacacccg gcagcctgcc ggggacgaag acgcagatga cgatccgctc
      241 gaaaacctat aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga
      301 acaggtctac atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac
      361 ggatgtgata aacaaccatg ctgagaccat tggcaacaat cagatgattg cggttaccaa
      421 caatcagata cagacggtgg gcgttaacca gatagagacg gtgggcagta accagatcat
      481 taacgtgggt tctgttcagg tggaaacgat tggacttgtt cgtgcgctga ccgtgggcgt
      541 ggcgtaccag acgacggtag gtggcattat gaacacttcg gtggctctga tgcagtcctc
      601 gcagatcggt ttgcataaat cgttgagggt cgggctgggt tatgacgtca aagtcggaaa
      661 taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat accgggcaga ccgcgattta
      721 ctccgccggt gagcatctgg agctctgctg tggtaaggca aggctggtgc tgacgaagga
      781 cggacaaatt tttctcaacg gcacaaaaat tcatttgcag ggtaaggagc aggttaatgg
      841 tgactcactg ttgattaact ggaactgtgc agcctcgaaa tccccaccga agccccctga
      901 tgaaaagcag gatacgccgg atatgagaga gtactgatcc cgtacctgtt cggaattcag
      961 taacaaacca gcattaaaaa taaacagaag aaggaatttg aatcatgagc ggaaaaccag
     1021 cggcgcgtca gggagatatg actcagtatg gcggtcccat tgtccagggt tcggcaggtg
     1081 taagaattgg cgcgcccacc ggcgtggcgt gctcggtgtg tccgggcggg atgacttcgg
     1141 gcaacccggt aaatccgctg ctgggggcga aggtgctgcc cggcgagacg gaccttgcgc
     1201 tgcccggccc gctgccgttc attctctccc gcacctacag cagctaccgg acgaagacgc
     1261 ctgcaccggt gggcgttttc ggccccggct ggaaagcgcc ttctgatacc cgcttacagc
     1321 tacgtgatga cggactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc
     1381 tgctgccggg ggaggcggtg tacagccgca gtgagtcaat gtggctggtg cgcggtggta
     1441 aggcagcaca gccggacggc catacgctgg cgcggctgtg gggggcgctg ccgccggata
     1501 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggccg tggtggatac
     1561 tcggctggtc agagcgggtg ccgggtgctg aggacgtgct gccagcgccg ctgccgccgt
     1621 accgggagct gacggggctg gcggaccgct tcgggcggac gctgacgtac cggcgtgagg
     1681 ccgccggtga cctgaccggg gaaatcaccg gcgtgacgga cggtgccggg cgggagttcc
     1741 gtctggtgct gaccacgcag gcgcagcgtg cggaagaggc ccgcacctct tcgctatctt
     1801 cttctgacag ttcccgccct ctctcagcct cagcgttccc cgacacactg cccggtaccg
     1861 aatacggccc cgacaggggt atccgccttt cggcggtgtg gctgatgcac gacccggcat
     1921 acccggagag cctgcccgct gcgccactgg tgcggtacac atatacggaa gccggtgaac
     1981 tgctggcggt atatgaccgc agcaatacgc aggtgcgcgc tttcacgtat gacgcgcagc
     2041 acccgggccg gatggtggcg caccgttatg caggcaggcc ggagatgcgc taccgctacg
     2101 acgatgcggg gcgggtggtg gagcaactta acccggcagg cctgagttac cgctaccagt
     2161 atgagcagga tcgcatcacc gtcacggaca gcctgaaccg gcgtgaggtg ctgcatacag
     2221 aaggcggggc cgggctgaag cgggtggtga aaaaagaact ggcggacggc agcgtcacgc
     2281 acagcgggta tgacgcggca ggaaggctca cggcgcagac ggacgcggcg ggacggcgga
     2341 cagagtacgg tctgaatgtg gtgtcaggcg atatcacgga catcaccacg ccggacgggc
     2401 gggagacgaa attttactat aacgacggga accagctgac ggcggtggtg t
//
LOCUS       DAZKGS010000321         2448 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKGS010000321 DAZKGS010000000
VERSION     DAZKGS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..2325
                     /gene="adiA"
                     /locus_tag="ACHOQY_004985"
     CDS             58..2325
                     /gene="adiA"
                     /locus_tag="ACHOQY_004985"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9680892.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          620 a          665 c          607 g          556 t
ORIGIN      
        1 aaaggccgga agatacttgc ccgcaacgaa gattccttca taaccgggta agcaatgatg
       61 aaagtattaa ttgttgaaag cgagtttctc catcaagaca cctgggtcgg taacgccgtt
      121 gagcgtctgg cagatgcttt aagccagcaa aatgttaccg tgattaaatc cacctccttt
      181 gatgatggtt ttgccattct ctcttcaaac gaagccattg actgcctgat gttcagctat
      241 caaatggaac atccggacga acatcaaaac gtcagacaat tgatcggtaa gcttcatgag
      301 cgccaacaaa acgtgccggt cttcctgttg ggcgatcggg aaaaagccct cgccgcaatg
      361 gaccgggatc tgctggagct tgtcgatgaa ttcgcctgga ttctggaaga taccgccgac
      421 tttatcgccg gacgcgccgt tgccgcgatg acccgctacc gccagcagct gttgccgcca
      481 ctgttcagcg cgctgatgaa atatagtgac atccatgaat attcctgggc agcgccaggc
      541 caccagggcg gcgttggttt taccaaaaca cccgccggac gtttctacca tgactactat
      601 ggtgaaaatc tgttccgcac cgacatgggc atcgaacgaa cttccctcgg ttctttgctt
      661 gaccatactg gcgcatttgg cgaaagcgaa aaatatgccg cacgcgtatt tggtgccgat
      721 cgctcctggt cggtagtcgt cggtacttcc ggctctaacc gcaccatcat gcaggcttgc
      781 atgaccgata acgatgtcgt ggtcgttgac cgtaattgcc ataaatccat cgaacaaggc
      841 ttgatgctga caggcgcgaa accggtctat atggtgccaa gccgcaaccg ctacggcatt
      901 atcgggccaa tctatccgca ggaaatgcaa cctgaaacct tgcagaagaa aatcagtgaa
      961 agcccgctga ccaaagacaa agccgggcaa aaaccgtctt actgcgtggt gaccaactgc
     1021 acctatgacg gcgtgtgtta taacgctaaa gaagcgcagg atctgctgga aaaaacctcc
     1081 gatcgtctgc actttgacga agcctggtac ggctatgcac gtttcaaccc gatctatgcc
     1141 gatcactatg ccatgcgcgg cgaacctggc gatcacaacg gtcctaccgt tttcgccacc
     1201 cactccaccc acaaactgct gaatgcgctg tcacaggctt cttatattca tgtacgtgaa
     1261 ggtcgtgggg cgattaactt ctcccgcttc aaccaggcct acatgatgca tgccaccacc
     1321 tccccgctgt atgccatctg cgcatccaac gacgtggcgg tgtcgatgat ggacggcaac
     1381 agcggcctgt cactgacaca ggaagtgatt gacgaagcgg ttgatttccg tcaggcgatg
     1441 gcgcggctat ataaagagtt caccgctgac ggtagctggt tcttcaaacc gtggaacaaa
     1501 gaagtcgtca ccgacccaca aaccggcaaa acctatgact ttgctgacgc accaaccaaa
     1561 ctgctgacca ccgttcagga ctgctgggta atgcatccgg gcgaaagctg gcacggcttc
     1621 aaagatattc cggataactg gagtatgctc gacccaatta aagtcagcat ccttgctccg
     1681 ggaatgggtg aagatggtga actggaagaa accggtgttc cggcggcgct ggtcactgcc
     1741 tggcttggtc gccacggcat tgtgcctacc cgcaccactg acttccaaat tatgttcctg
     1801 ttctctatgg gcgtaacccg tgggaaatgg gggactctgg ttaacaccct ttgttccttc
     1861 aaacgccact atgacgccaa cacaccgctg gcgcaggtga tgccggaact tgttgaacaa
     1921 tatcctgaca cttacgcgaa catggggatt cacgatctgg gtgacaccat gtttgcctgg
     1981 ctgaaagaaa acaaccctgg cgcacggttg aacgaagcct attccggcct gccgatggcg
     2041 gaaatcaccc cgcgtgaagc gtacaacgcg attgtcgaca acaatgtcga actggtatcc
     2101 attgaaaatc tgccaggacg catcgcggca aactcagtta tcccgtatcc gccaggaatc
     2161 ccgatgctgc tgtctggtga aaacttcggc gataaaaaca gtccgcaagt aagttattta
     2221 cgctcgctgc aatcctggga ccaccatttc cctggatttg aacacgaaac tgaagggact
     2281 gaaattattg acggtattta ccacgttatg tgcgtgaaag cgtaaccact attccgctga
     2341 aggcgtaatt gtttaaataa cattacgccg cctggcctta ggccttttga gtatggcaac
     2401 gttttcataa aaattgctgc aaacaaaaat gtcatacttt ttgcgcgg
//
LOCUS       DAZKGS010000322         2414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKGS010000322 DAZKGS010000000
VERSION     DAZKGS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..66
                     /locus_tag="ACHOQY_004986"
     CDS             <1..66
                     /locus_tag="ACHOQY_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9680893.1"
                     /translation="AQRCVNHISEPDTEIPPGFRG"
     gene            73..819
                     /locus_tag="ACHOQY_004987"
     CDS             73..819
                     /locus_tag="ACHOQY_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9680894.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNMRVVK"
     gene            841..1611
                     /locus_tag="ACHOQY_004988"
     CDS             841..1611
                     /locus_tag="ACHOQY_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9680895.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGGNLPADPKAAPVTENDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KGQVQKWERVCAALRELNKHRDIVRQIVDSSGRIVSEK"
     gene            1627..2022
                     /locus_tag="ACHOQY_004989"
     CDS             1627..2022
                     /locus_tag="ACHOQY_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9680896.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFTSEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRQQ
                     NIICGECRKSEVMLRVLAFYQGNFQEAIL"
     gene            2080..>2414
                     /locus_tag="ACHOQY_004990"
     CDS             2080..>2414
                     /locus_tag="ACHOQY_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680897.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEASERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRA"
BASE COUNT          670 a          505 c          721 g          518 t
ORIGIN      
        1 gcgcagcgtt gcgtgaatca catttctgaa ccggacactg aaataccgcc gggattcagg
       61 gggtgacgtg tcatgaaaaa cattgcggca gttggggttc ttgaacgtat tcgcagactt
      121 gcaccacagg gggcggttcc accgtaccgg acggtggagg agtggcggga atggcaactt
      181 gctgaaggac gaaaacgcag cgaggagatt aaccgccaga atcgccagtt gcgggtggaa
      241 aaaatcctga atcgttcggg catccagcct ctgcacagca aatgctcgtt tgcgaattat
      301 caggtgcaga acgacgggca aaaatacgcg ctgagccagg ccaaatccat agctgacgaa
      361 ctgatgaccg ggtgcacgaa ttttgtgttc agcggtaaaa ccggcaccgg gaaaaatcac
      421 cttgcagcgg cgatgggtaa ccggctgatg gcgaaggggc gcagcgtgat tatcgtcacc
      481 gtgtctgatg tcatgagcgt gttgcatgac agctacgaca acggcaaatc cggggaaaaa
      541 tttttacagg agctttgcag tgttgatttg ctggtcctgg atgaaatagg cgttcagcgg
      601 gagacgaaaa acgagcaggt ggtattacac cagataattg atcgccggac agcatcactg
      661 tgcagtgtcg ggatgttaac aaacctgaat catgccgcaa tgagtacact tcttggtgag
      721 aggattatgg accgcatgac catgaacggt ggtcgatggg tgacgtttaa ctgggatagc
      781 tggcgtccaa atgtcagcaa tatgagggtt gtgaagtaat tttgtccgga ggaaatttta
      841 atggaaaccg tatctgacgc actgaaagca ctgaaaaaag cctcttcaca tgtggtggca
      901 gctcgccttg gaatcagtcg tgaagaggct gtcaacgagc tgtgggaact caaaagaaat
      961 ggcgtcgttg ataaaactgg tcacacctgg tttctggctg gcgaaggtga atcccgggta
     1021 accgaagagc ggccagtaaa atctgaagca caggatatgc tgaccgggga ggtcgaacaa
     1081 aaagttaccg cagacatgat gattgagttt atcggtcagg atggggctaa aacgtgtgag
     1141 gaactggcgg ataagttcgg tgttagcatt cgcaaggttg cttccacgtt ggcggtcgta
     1201 acagcaacgg ggcgcctggc acgcgtaaat cagaacggta aatttcgtta ctgcatgccg
     1261 gggggtaatt taccagcaga tccgaaagcc gcgccggtaa cggaaaatga tggtaaggcc
     1321 tttcctcagc cagcaggtgc tgcgttacca gtccgggaag ccgcaacaca ggaagaaatt
     1381 aaaacagaaa ctgtggcgga cattgtgcag ccgttgccat cgtttaccga aacgcaagca
     1441 gatgagctga tttttccgtc ccttcgcagg gcaaacctgg cgctgcgcag ggcaaaaggt
     1501 caggttcaga agtgggagcg tgtctgcgcc gcgctgcggg agctgaacaa gcaccgggat
     1561 attgttcggc agattgtcga ttcatccggt cgtattgtgt cggaaaagtg attgccggag
     1621 gcgcttatgg cgaaaccttt tacacacgaa cagcgtgaag aactgaaggc ccgaattatc
     1681 gggctggtac gcaaaaatga acgcatgacg atatcacaac tggagagagc gacgggagca
     1741 ggctggcatt cagtcagacg ttgccttgtg gatgtactgg cttgtggcga tttatacatg
     1801 cccggtaaat acggtgtttt tacatcagaa caggtgtatc gcgtatggcg taaggcagcg
     1861 gagaaaacaa ccgaccagac attgattcga aagttaccag acggagaaat acgccgctac
     1921 gacagacaac agaacataat ctgtggcgag tgccggaaga gtgaagttat gctgcgtgta
     1981 ctggctttct atcagggtaa ttttcaggag gcgatactgt gaatgaaatt agctatcagg
     2041 cttcaattac cgctggcatt cacatcaaag gagaagagca tggaaataaa accagaagat
     2101 gagttaagca atatcgtttt atttccggta aaagaggatg acccacgtaa tcaggttaat
     2161 tttctttatg aggcatcgga aagagcatat tgtcatcacg cctctgttcg ggttgacgaa
     2221 aaagagcgtc aggtccgctg taaaatctgc ggtgcagttg tggagccgtt tgactggatg
     2281 ctctctgtgg cgaaaagaga aaccagactg gcagatgatg taaggctctt gcgccaggag
     2341 gagcgggaaa ggcgaaaaaa tatagaaaag ctaattcaga ttgagcgtaa cgcgaaagcg
     2401 cggatacgca gggc
//
LOCUS       DAZKGS010000323         2370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKGS010000323 DAZKGS010000000
VERSION     DAZKGS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(437..513)
                     /locus_tag="ACHOQY_004991"
     tRNA            complement(437..513)
                     /locus_tag="ACHOQY_004991"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Arg,seq:tct)
     gene            complement(527..603)
                     /locus_tag="ACHOQY_004992"
     tRNA            complement(527..603)
                     /locus_tag="ACHOQY_004992"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(567..569),aa:Arg,seq:tcg)
     gene            complement(613..688)
                     /locus_tag="ACHOQY_004993"
     tRNA            complement(613..688)
                     /locus_tag="ACHOQY_004993"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Ile,seq:cat)
     gene            complement(729..842)
                     /locus_tag="ACHOQY_004994"
                     /pseudo
     CDS             complement(729..842)
                     /locus_tag="ACHOQY_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1191..1943)
                     /locus_tag="ACHOQY_004995"
     CDS             complement(1191..1943)
                     /locus_tag="ACHOQY_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9680898.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     ATLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            complement(1957..>2370)
                     /locus_tag="ACHOQY_004996"
     CDS             complement(1957..>2370)
                     /locus_tag="ACHOQY_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9680899.1"
                     /translation="VVPSVTRESDLVPSVPATSIIQDKAKKVLVLKVDPESPESFMLR
                     PKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQCGMGTKAHDLFVLPLCRKHH
                     DELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
BASE COUNT          624 a          632 c          509 g          605 t
ORIGIN      
        1 agaaatgaaa aaaggccacg cagttgcgca gcctgataaa ccctggttaa aatccacacg
       61 ataaaaatga caatgcaagt atctcatgct gttgcccgaa cctactcggg ctttttttgc
      121 atgtaaaaag gctcctgcga tgaggggcct ggatatatgc ctaatctctg tatacagcat
      181 gatgccgggt gcctcccggt gaattctgca atgaccagac agaatccgca acttgcctat
      241 acaatacgca accaaacatc tgtcattatg ccccgccgcc cagggggatt catcatgcag
      301 gattttttta acaaacgctc agcatgtcag gcaacagtcg actacctgaa ttgtgaggca
      361 tttaacattt cactgtccgg tgtctttcct gtaataaaaa gcccgcaaaa gagagtcagg
      421 gcagataagt gtggtgtggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa
      481 ggcaattgct ctgtccggct gagctaacaa cgcatgatgc tgataatgga ccgccatcgg
      541 ggacttgaac cccgcacagc cagcttcgaa ggctgacgct ctatcccgat gagctaatgg
      601 cggtatgtga tatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg
      661 ctcgctctca ccactgagct aaagggccgg gcgcaggata ataacggtac gtaactaatc
      721 ctgcaatatc atccgttctg actgactaaa tcctgaactt ccctgaccgt ctgctcaaaa
      781 cgttcagtct ccagctcaac gccaattgca cgacgcccca gcgacattgc tgctttgacg
      841 ctacagacat aaaaaagcca gccactgggg gaggctggca aactcgtaga gcaaaatgct
      901 gttacgcaaa cttcgttaca gggtcatcct gcaatacaaa aaatacacaa tatttagaaa
      961 actaatagtg ccatgtgcaa tttttaagat tttgttatta attgtggtcg caccttcctt
     1021 tctgtgtact ttccgtatag ctcacaggat tctgggtaca aaaaaacccg cgcatcggcg
     1081 ggttcttaaa tcttatcaac ggtagacata caaagcccat cgttgggaaa atcttatcca
     1141 tattttttga aaaatgcaag catcatgtcg tcatcttcgg cgaaaaccat ttatcttgtc
     1201 acctttctca attgtatctc tgcatatgct tcttcctgcc agcactttgt aaccagttta
     1261 tcaatgacat ctgcatatcc tttgtaccac tgataatccg tcaggtctgg taccagcttc
     1321 tggacatgaa gccgcgccag tgtggttggt aaacggctaa accggtttcc attgcaacgc
     1381 ccacaaacct tataaacagg cgtgccatga agccgggttc ttttttcatc caggacaata
     1441 cctttaccct tgcaccctct gcatgctgtg ctgacttctc ccttaccatg acaatgctga
     1501 catagttcct tcacccactc ttccttaata acagattccc cgcttctgga gtgtttcacc
     1561 acctcgcgca atacattatg aaatccagta ccagcacaat gctcacagcg agccttactt
     1621 gccgcagacc tggaataatc agcaaaggca aaattcacaa ggtaaggaat gatctgtagc
     1681 cgggtttctt cactcaattt attcaatgtc gggttatcca gtgccatcgc gtaattgagc
     1741 agaccttcaa tcgcaaactg aggatcctga acaccaactt ttgccaggaa taaagcaaac
     1801 ccaagcgttg cttttgactg caccatcccc tgcgcagcca tcacatccgt aattgttaaa
     1861 ccaccagagc ctgtcgccgg tgcgtcatcg ctcagttttg gagatttcgg ggagtaatat
     1921 ttcggtaagg cttcaaggtt catgctcgtt ctccacttac gccagtacgc caattgccag
     1981 cgcacgatcg ataaaacgaa atatcagctc cagctgagag ccatacttct cttcaaatgc
     2041 cacggtatcc gcatgcagct cgtcgtgatg ctttctgcac aaaggcaaca caaaaaggtc
     2101 atgcgctttt gtacccattc cacactgacc gtggcctatc aggtggtggg gatcatcagc
     2161 gggctttcca caacatgcac acggctgtgt cttaacccag cgcgtgtact tttcattaac
     2221 ccagcggcga cgttttgggc gtaacataaa agactccggc gactccggat ccactttcag
     2281 caccagcacc tttttcgctt tatcctggat gatgctggtg gcaggaaccg aaggcacaag
     2341 gtcactttcc cgggtgacag acggcacaac
//
LOCUS       DAZKGS010000324         2363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKGS010000324 DAZKGS010000000
VERSION     DAZKGS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..512)
                     /locus_tag="ACHOQY_004997"
     CDS             complement(<1..512)
                     /locus_tag="ACHOQY_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9680900.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGEASESAR
                     QAAESAASAK"
     gene            complement(664..1263)
                     /locus_tag="ACHOQY_004998"
     CDS             complement(664..1263)
                     /locus_tag="ACHOQY_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9680901.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDDPKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYDGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1331..>2363)
                     /locus_tag="ACHOQY_004999"
     CDS             complement(1331..>2363)
                     /locus_tag="ACHOQY_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9680902.1"
                     /translation="FFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQ
                     TVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAG
                     MQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFS
                     LTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFP
                     RDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKN
                     GAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMK
                     KATAGITIS"
BASE COUNT          531 a          693 c          540 g          599 t
ORIGIN      
        1 tgctttgctg aggctgcact ttctgccgcc tgccgggctg actccgatgc ctcccctgct
       61 gaagtgtcag catttgcagc gctctcttct gcctgactgg ctgatatgcc ggcattcctc
      121 gctgacgtct ccgcctctcc ggcattcttc ttcgcctcct cagcgtgacg cgccgcttct
      181 tccaccatca gttcaaaacg acgcagtgcc tccggccgga cgtcatcctc cgacatggca
      241 ccgagaaaat cattcagcgt ccccggttga gaatcttcat acacggtgat ggtcccggca
      301 tgtgacggag gaaatccctc caccaacaga atgacgctgt actgaccgta ctcaacgtcc
      361 atgctgtaac gtccggcttc atccggattt tcagaggcca ccgtgttcac caccaccgtg
      421 ctgctggttc gtctggcctt cagcacaatg gtgcagttct gtactggttt tcctgtgcca
      481 tctttaagca cgccagaaat ttttactgtc atacttttcc accaataaaa aaagcccgca
      541 gcagtgacgc cacgggcttc aggacagtgt aactttacgt ttcctcaaac gcagttcacc
      601 ccataaggtg gatgaacctg cgtatcataa caatatttac agaagataaa tcggcgtctg
      661 ttgtcagaaa cggtatccga taccaacaat aaatgcatcc gatcgccagt cgccactacc
      721 ggaaccgtca taagcaaggt caatggtcac ggattcggtc gggttaaact gcacgccagc
      781 cccccacgcc agagacgtgt tgctgtggcg accgtcatca cttccggtca gcacatcgtg
      841 cgttttcccc ttgttgtcag ttacgcggag ataatccccg gagaaagtcg acacacggct
      901 gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgta cagacggccc
      961 cgccatcacg ctgaaccagc ggttacgcac ggaatcttca tgccagcggg tatcgctgta
     1021 gcgcgttttt gggtcatcct cagcattggc ataactgaag gacgtaatca gccccagcgc
     1081 gtccataaac tcataacggt atttcacgtt aatcccgttc agatcatcac taccgggaac
     1141 gttcgtcgag gcatggagat accccgcgct cagcgtggac tgatgttctg ctgcactcgc
     1201 tggcgtagca gcggcgacct gccagactac tgcggacaaa ataacagcac ataatttacg
     1261 cataattacc tctcgctttt ctgcaataaa aaaggcgcca tttctggcgc ccgtatctgg
     1321 gttataaaat tcagctaatc gtgatgcctg cagtggcttt cttcatcacc acaaccagca
     1381 aatcgctgat acttgctgtg ggataccagt tatttaccag ccatgctgac accgaaaact
     1441 ccagtgtcat gtgaccgtga ccggcaggca tatcaataac accactgtaa atcagcgtat
     1501 tatccagcgc ggtacggtta taaatttcag caccgttttt ccgcactatc agacggcatg
     1561 aggagtaaat atcagtatgc tctttctcat gtttagcgcc gctgaatgcc accgccggaa
     1621 taacaatctg ccggtcaaac ggctgatcgt cataaaccct gacggtaatg gttcctgatg
     1681 gccaccgctc cggtgcacgg gagtcccggg ggaaagcttt gcccactgtt ttaacgagat
     1741 cgccttcaat ctggttcgcg gacaattttc ccagaacccg acagttctcg ttaatcgtga
     1801 cgttgttgag cgtcccggag ttcgcattca cgttaccgct gatatcggca tttttcgccg
     1861 tcagccgccc gtccggtgtc agggaaaatg ccggaggatt accgccgctg gtaatggtgg
     1921 gagccgtcag atatttcagg aacacttcat tcataaatat ctgatcgccc tgaccaacaa
     1981 acatcggctt tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct gccgccagca
     2041 gcacctgact ctgcattcct gctggcgtat tctcaatacc ggcaccgata cccgcaatat
     2101 aaaggcgtcc gtcctgcatc tgctgcagtt tcacggccca catgctgttc aggttattat
     2161 ttgtatcaac ctgaactttc tgtatctgct ggattgccgc actctgattt tccagttttt
     2221 tattgacggt ctgcgtgatt tcattgctga cattcgtaat ggacgtcctg atttcagcca
     2281 ggtccggcgc aagctgaccg ttatcaatct gcgtccacag ctcctgggcc agatgtgttt
     2341 tcccgatttc tcctttgaaa aaa
//
LOCUS       DAZKGS010000325         2359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKGS010000325 DAZKGS010000000
VERSION     DAZKGS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..587)
                     /locus_tag="ACHOQY_005000"
     CDS             complement(<1..587)
                     /locus_tag="ACHOQY_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9680903.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLV"
     gene            complement(645..992)
                     /locus_tag="ACHOQY_005001"
     CDS             complement(645..992)
                     /locus_tag="ACHOQY_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9680904.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(1029..>2359)
                     /locus_tag="ACHOQY_005002"
     CDS             complement(1029..>2359)
                     /locus_tag="ACHOQY_005002"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9680905.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          466 a          754 c          602 g          537 t
ORIGIN      
        1 ttcacaagac cgctggcctg gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg
       61 tcacgatcct gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac
      121 ctgccaaaat cgacctcaat tttctcgaac tggtctcctt ccatcgtgta tttgccatac
      181 agcacggcat tcaccgcctg catttcttcc acccggacaa tggcgtgctc ttcctgtttg
      241 aggttatcag tgatgatacg cagacgacgg taggccgggt cgttcagctg agccggatct
      301 tcaccgggaa gacgctcaac cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag
      361 cccggacgta acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc
      421 ggggaaacat aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg
      481 gtatggaaat tcaccgtacg gcggaaaaac agctccagaa acagcgcacg aaatttcact
      541 ttttgttcgg tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt
      601 tcattcagaa atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg
      661 tgactcaggg cactgccggc aaaggcgttg gcctttttgt gttcatccac actttcaggc
      721 cagcggattg cctccgtcgc aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg
      781 ccttcaagcg gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc
      841 accagtttcc cggtggcctc atccagcatc aggggcgtca gtgccggtgt tgccgaggaa
      901 atcccgctgc tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc
      961 acacgctgtt cagtgatagt tttcgtcacc atattgtaaa aacctcctgt tgatggtcag
     1021 cactggcttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc
     1081 tgtggcacgc caccctgtac cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg
     1141 gcggttgtgg atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg
     1201 gcgctctcca cagtcattcc cgggcaggca gccagctgtt cagcctgcgc ctcagcccct
     1261 tttgcctcat ccagtgccat gatctgatca cggagtgagg gtccggcatc cgcctgcggt
     1321 gaagcagcca ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt
     1381 gccgccagtt gttcacgacc tttcgcttct tcacacgcca taatgcgatc ggcttcactc
     1441 tgcgcggatg ccaccggctg ctgtggtgcc gccgcggcca gaatcgcccg ggcctgttca
     1501 acgctcatgc cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc
     1561 tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt
     1621 aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg
     1681 gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca
     1741 aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata catcgctact
     1801 ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg gcgaacctct
     1861 gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg
     1921 atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca
     1981 atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc
     2041 atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg
     2101 cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg
     2161 tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaatg
     2221 ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg caccagcgtg
     2281 ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc cggtcgttta
     2341 cctccggcca tgtactcgt
//
LOCUS       DAZKGS010000326         2351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKGS010000326 DAZKGS010000000
VERSION     DAZKGS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2351
                     /gene="agn43"
                     /locus_tag="ACHOQY_005003"
                     /gene_synonym="flu"
     CDS             <1..>2351
                     /gene="agn43"
                     /locus_tag="ACHOQY_005003"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP9680906.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAA"
BASE COUNT          567 a          630 c          738 g          416 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggattcgtc
     2281 cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac cgcgggggta
     2341 tatggtgctg c
//
LOCUS       DAZKGS010000327         2348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKGS010000327 DAZKGS010000000
VERSION     DAZKGS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..573
                     /gene="espM1"
                     /locus_tag="ACHOQY_005004"
     CDS             <1..573
                     /gene="espM1"
                     /locus_tag="ACHOQY_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP9680907.1"
                     /translation="GVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSIRFNVNK
                     FSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEERKMVFS
                     LVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYIANVLAD
                     KFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(792..1848)
                     /locus_tag="ACHOQY_005005"
                     /pseudo
     CDS             complement(792..1848)
                     /locus_tag="ACHOQY_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2080..>2348
                     /locus_tag="ACHOQY_005006"
     CDS             2080..>2348
                     /locus_tag="ACHOQY_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFP9680908.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFL"
BASE COUNT          641 a          459 c          553 g          695 t
ORIGIN      
        1 ggtgtgtcct tcagctcttt tggtatcagt tatcataaag ataattcttt ccggggcacc
       61 atccgtggga agaatgatga ggtcgtgaag tgttcaatgg gagagcgctc tatccgcttt
      121 aatgttaaca aatttagcgg ctgtatactg gagacggtaa gcaggcagag tactaaagat
      181 attcatggat gggtaagtga tgaacggaca gtatatccgt caagggttat caaccaagag
      241 attgataatt gctgccttca aaaaaatgca aaaatttctt ctgaagagag gaaaatggtc
      301 ttttctcttg taagtaagga gtttgaacta actcttgatg ttaaagcggc acaaagttct
      361 attaatcata taataatagg aaatgcttct tttggcaaaa aaatggatgc tctttgcgat
      421 ggtatgagcc gagctgtaaa aaacagtaca acagattaca tagcaaacgt gcttgcagac
      481 aagttttatc agaaacatat cgccccgggt gttgatattg taaaactacg aaatgaaatc
      541 ccaggttata tgagtcgtgt tatacagggg taaggttgga ggaggctatg cgccagtatc
      601 attggttttc atcctgtaaa aagatatgag taaaatatac aacaggcgtt acactatatg
      661 tatgtatcca aataactcat agttcacctg tacagttata aatcgtaaag aaaattgcag
      721 cgcgtcgtac gcagaaacgt gctgtgattg actatttata gctttgtcac aacgacaaga
      781 ttcagtagtg tttacacggg cgggagaaat gacggaataa ctaaatagct gcgcctaata
      841 tcgcacattt ttgccagccc atgtttgcct tcggggaatg gactgacgga ttccataaaa
      901 tggcgaactt tttcaatagt tgccacattg agcggcactg atgattacgc gttattgtgg
      961 cgtgaagtat ctgccatagc tattcaaaat gattcaccca tggagattaa accggctgat
     1021 aaatgactct gcacttcggt ttatccttca gccagcgctg ggtttcccgg cctttgtgga
     1081 taatgtagtt gtcctcgagc agcgtgatgg ttttcgtccg acagtatgta gctttaagcc
     1141 gcttcagcag tctgatgaac agcgccgaac ttttgctgtt ggcgcccaca cagctgactt
     1201 aacctgtccc gctgtgcagc gctacgtcca gagaatattt ttcattttgt tccggcgtga
     1261 cccctcgttt ttgcttcccg agcagtttcc agtccgcacc gattttggga ttaagatgga
     1321 taaatacttc atcttcataa aagaccggat gctctgtgct gcattcgtcc agtgcgttat
     1381 ggattgttgc catcttttta tctttaggcg gggcacggat acgtagagtt ggcgcagccc
     1441 ttcgccacac aagccccaca gacgacaacc agcggcgaat ggttccagca tgtaacaggc
     1501 agccggttat ctcattgatt tttattgtca gcagttctgt actccagcgt gaataattgt
     1561 agccaaagtc gcgagtgaat attttacctg ttcacgtaat agtgtgtaga tatgcgcaaa
     1621 cggccagcgg cgggctcgct cggcaggtaa tgatttcagt ccctcaacac ccgactgtgt
     1681 gaactagtta atccagcgcc caacagagga acgggcgcag catagtgtgc gggcaacgtc
     1741 gctgacacgg tcgccccggt gcagcatcag catggctgtc agccttttat ggatggcttt
     1801 ctgtattagg cgtcgttcgt cacgggaaat tggtggtatg atcgacattg ctcagtccgg
     1861 ttggtgattt gtattgattt ggcgattgat cagatcgcac aatcgggttg agttccctct
     1921 aaatgatcta ctattctgcg cagctattta gtgagtatat attttatgct tataaattag
     1981 attaaaataa tcctacaatt attctgatgg agtattgctg tggaggatgg ctttttgtta
     2041 attatcgaaa agtaagatat atgctttagg gataaaatta tgccattttc atttaatttg
     2101 tcttctggaa attacttgtc aacacaagat gttgaagttt tacaacgtgc gaccagagat
     2161 catcaaatgg aaagactcac aataggagaa agatcgtttt ctgttcgcta tcagagtgct
     2221 atggacgctt ttattgtaga tcctgttcaa ggggaactat attcgggttt aagccataca
     2281 gaactagccg atatcattag attggctgat tctgttgaaa atcaattgaa tggaggcaat
     2341 tcatttct
//
LOCUS       DAZKGS010000328         2345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKGS010000328 DAZKGS010000000
VERSION     DAZKGS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..86)
                     /locus_tag="ACHOQY_005007"
     CDS             complement(<1..86)
                     /locus_tag="ACHOQY_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9680909.1"
                     /translation="MINQLTFTKHYDTFDNVSKIYSDKFPQG"
     gene            complement(140..310)
                     /locus_tag="ACHOQY_005008"
     CDS             complement(140..310)
                     /locus_tag="ACHOQY_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680910.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(330..518)
                     /locus_tag="ACHOQY_005009"
     CDS             complement(330..518)
                     /locus_tag="ACHOQY_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680911.1"
                     /translation="MKWFTPEHVVQAFKKGELTRHQVVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
BASE COUNT          697 a          445 c          407 g          796 t
ORIGIN      
        1 tttccttgag ggaatttatc agaataaatc tttgatacat tatcaaacgt gtcatagtgt
       61 tttgtaaaag ttagttggtt aatcattaaa aatctccttg atgtaatcac gctctatggc
      121 gctactatgg tgataaaaat cacttgcttt tatttgagag tgtttcagcc tctcttttca
      181 acatatcaag aatgtaatct gtgacagtga tccgtctact tctggcttct ttaaataaca
      241 cctgaaatac ttcatcagga atatgaaagc aagtgcgtgg ttgtcttttc ttttctgtag
      301 tttcgctcat agttttctcc ttctcttctt tactctgttt cagattcttt ttcatttttt
      361 cttagttcat caataatctt gagtgcttca ttgaagcaag ctgctcgctc tggataacct
      421 cgactccttg ccatattacg attcattact acttggtggc gtgtaagctc acctttttta
      481 aatgcctgga cgacgtgctc aggtgtaaac catttcataa aaaactcctt attcagttaa
      541 aagaaaaaac accttgcaag cctgacggct tcaaagtgca ttgattttga ttatttagag
      601 aagaatgttt ttctgtccct ctaataccta ttattacata taaagaaaca tttgtcaata
      661 ccttttcata aaaaatctga tctttttcat gcacttacat tttttcttag caattcgtgc
      721 tctcctatgt agtgctattc ttccttattg tctaaaacgc tctaaaacgc gttctaacga
      781 gattttgata aaagcaatac attcccttat ctaatggctg tttagctcgc cagaggctta
      841 tttaagtggc ttatttaagt ggcttatttt gtatttgtta gatgatgata gatataaaac
      901 agagagtcac tttataaaac gctctgtaaa gccctgtagt gagtttatta tatgtaggca
      961 atgcgtttcc attccctacc ctgcttaatg ttgttagaga agcatttaga ccccttattt
     1021 ttaattacct aaagtatcct ctatacatct ctgttatgaa acacctctga atccttcata
     1081 aaacacatta tgacgcaaag aacacttttc taaggtgatt cccttcctga atgctgtttc
     1141 ttgcgtcaga gagctacaca gagcttataa agatattatt aataataaag tagataaagt
     1201 aagtaagata agtaagtaag ataactaaga tagataagat taataagatc agaattctct
     1261 ttcggtgtcg cctctggctt ttccaccgaa aacaattaca cgactacaga atgtaggaag
     1321 tgaaattgta ttgaggctta gtgaaattac acgaggaaag cagagtgtaa ttgagcttta
     1381 gccgctctct tcgagtaatt acacaactac agagaataac gagagttatt aaatgtagga
     1441 agtggaatta agaggtagag agttacacat aagcaatcag tatgattggt taggtgatta
     1501 ctcttctgcg cacagcgcaa tagtatttct tcgtgggcgt taccgccacg atagttacac
     1561 gaagaaagta acgctttgct ttcggtgtga gcggaacggg ccagccacga agcccgaaag
     1621 gtagcggagt aaggctgctt gccagagagt gaagctaacg ccggaaggca aaagatcata
     1681 tatatatagt ataaagaata ttattatgat ctaattaata atctaatata gttttattaa
     1741 tagtcttatt gtagttcact gtgacctacc ccctagctca ctatgaccta ccccctagct
     1801 cactatgacc taccccctag gtcacagtga cctatatagt ataaagaata ttatttatat
     1861 tatttaatta tagtattggt ggaactcatt tcctaggtag gtaggaactc atttcctaag
     1921 taggtaggaa accacttcct aggttacttc ctaaatagat cggaaatcat ttcctaggtg
     1981 ttatttattc ttatcgggta tcagttttga taggggtatc agacttgata ggggtaacag
     2041 aattctgtgg gggtagttca ctatgaccta cccctagctc actgtgagct accgttacat
     2101 tcaatcatct tccgatgtgt aatatcgtta cattcaataa ttagtctatg taacatatcg
     2161 ttactaccaa ctttcttaca aagagcattt cagatagctg aatgtaacct ctcgttacat
     2221 ccatttgtca catagcactc tgtgtaatgc ctttctatgt gtgtgccata atccccccta
     2281 tattccctgt gtaaatcata gaatatccct aacttattcc tgtgtaattc catactaaaa
     2341 attca
//
LOCUS       DAZKGS010000329         2335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKGS010000329 DAZKGS010000000
VERSION     DAZKGS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHOQY_005010"
     CDS             <1..224
                     /locus_tag="ACHOQY_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9680912.1"
                     /translation="KRNHPHADAIHVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQ
                     NHDSSVVQLQQRIAGLEKQLADIKSSSAE"
     gene            230..1366
                     /locus_tag="ACHOQY_005011"
     CDS             230..1366
                     /locus_tag="ACHOQY_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9680913.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1363..>2335)
                     /locus_tag="ACHOQY_005012"
     CDS             complement(1363..>2335)
                     /locus_tag="ACHOQY_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9680914.1"
                     /translation="TKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFE
                     SKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAP
                     TGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDES
                     LVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSK
                     EVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWWL
                     DLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT          573 a          551 c          606 g          605 t
ORIGIN      
        1 aaaaacgtaa tcatcctcat gccgacgcca ttcatgttgc tggttgggtg agtcttttta
       61 cgctgcatgt tatctggcca tttttgtgga tttgggccac gctttaccgc ccggagcggg
      121 gatggggaat gcaaaaccat gactcctccg ttgtgcaact acaacagcgt attgccgggc
      181 tggagaaaca gctcgccgac atcaaatcct cttctgccga gtaatactta tggatttact
      241 gattattttg acctatgtgg cttttgcatg ggcaatgttt aagatcttca aaattcctgt
      301 aaataaatgg accattccca cagcggccct gggtggcata tttattgtca gtggtttaat
      361 tctgttaatg aactataacc atccgtatac cttcaaagcg caaaaagcgg ttatttctat
      421 tcctgttgtc ccacaggtga caggcgtggt gatcgaagtg acggataaga aaaatacgct
      481 gattaaaaaa ggtgaggtgc tatttcgact ggacccgacg cgttatcagg cgcgggtgga
      541 tcggctgatg gcggatatcg ttaccgcaga acataaacag cgggcgttgg gcgcagagtt
      601 agatgagatg gcggcgaata ctcagcaggc aaaggccacg cgggataaat tcgctaaaga
      661 gtataagcgt tacgcacgtg gcagccaggc gaaagtaaac ccgttttcag aacgcgatat
      721 cgatgtggcg cggcaaaatt atctggcgca ggaagcctcc gtgaagtcat cggcggcgga
      781 acaaaaacag atccagagcc agctggatag cctggtgttg ggtgaacatt ctcaaatcgc
      841 cagcctgaaa gcacagctcg cggaagcaaa atataacctt gagcagacga tagtgcgtgc
      901 gccgagcgat ggttatgtga cccaggtact gattcgtccg ggtacctatg ccgcgtcgct
      961 gccgctacgt ccggtgatgg tgtttatacc cgatcagaaa cgacaaatcg tggcgcagtt
     1021 ccgtcagaac tccttgctgc gactggctcc tggcgacgat gcggaagtgg tgtttaatgc
     1081 tctgccaggt aaggtattca gcggtaagct ggcagccatt agtccagccg ttcccggcgg
     1141 agcttatcag tcgaccggca ccttacagac gttaaacaca gcgccgggtt cagatggcgt
     1201 tatcgcgacc attgaactgg atgagcacac tgatttgagc gctttaccag acggtattta
     1261 cgcccaggtg gcggtttact ctgatcattt cagccatgtc tcggtgatgc gcaaagtgct
     1321 attacgcatg accagctggg tgcattacct ttatctcgat cattaatctt cgctttccgc
     1381 cgcttgttca ggccgcagtt gctgcgactg agcaagctgg tggcgaattt cacgcagcat
     1441 ttcatccagc acatactgga cccgccaggg ctggtattcc cgtttacgga agatagccac
     1501 cagatccagc caccattcat agggttgctg aaagcagggc accagcgtgc cgtcttgcag
     1561 gtcgctctgc acacttttgt ctggcgcaaa cacaatcccc aggtgatttc gtgccagctc
     1621 cagcgcagat tgcgtgttgt cgcaaacgta atttcccttc acgcgataat cccgcacctc
     1681 tttactcccg gcgacgttga agcgccagat attcgcgtcg tcgatcatca tcgagtcgat
     1741 caaaatacag gagtgatgtt cgagttcgtc aggacggcta atgggatgtt tctcaagata
     1801 gcgctggctg gcataagcgg ttacggcata ttgggtaata aaactggcaa ccagcgattc
     1861 atcttttggt ggagcgtagc tgattaaaac atcacaatca tcaggaaatt cgacgccttc
     1921 ataaaaggca ttacgctcaa gattgcaggt ttttagcgat aaggtgatat caccgatatc
     1981 tttaatttta tcgattacat gtttggataa ataggtaatt atgccggttg gggcgtagat
     2041 ggtgacccga ccacgtttct catgcttata atctgcaata aaattattaa gttgctcgtt
     2101 tctgtccagc atgtcgttga tgtacggtag cagcgcggtg ccaaaaggtg tgagcatcag
     2161 ctgccgggtg gttcggtcaa aaacttttaa acccactttt gattcaaaat cagcaagata
     2221 tttgctgacg ttggcctgtg cgatgccaag tacagtcgcg gcatggctga tattttcact
     2281 ggcagcgatt accgagataa tttttaactc ccggtactta agttgtaatt ttgtc
//
LOCUS       DAZKGS010000330         2321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKGS010000330 DAZKGS010000000
VERSION     DAZKGS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            351..542
                     /locus_tag="ACHOQY_005013"
     CDS             351..542
                     /locus_tag="ACHOQY_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680915.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(595..828)
                     /locus_tag="ACHOQY_005014"
     CDS             complement(595..828)
                     /locus_tag="ACHOQY_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680916.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(924..1547)
                     /locus_tag="ACHOQY_005015"
     CDS             complement(924..1547)
                     /locus_tag="ACHOQY_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9680917.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1636..2145)
                     /locus_tag="ACHOQY_005016"
     CDS             complement(1636..2145)
                     /locus_tag="ACHOQY_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680918.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          674 a          593 c          496 g          558 t
ORIGIN      
        1 ttattctgac aaatatcgca aaaacagcac attacaaaat ttataaccat aattactgga
       61 aaagtgcaga aaattattcc actgaatgct tttaatacag ttggttagct taaaaaatag
      121 caatttttga tttcagtcat tgaaagttgc aatgtttgac gattcaacag catttaagtg
      181 agtttcagga tatgggaagg acatgaaagg aggagcgcaa taaaatacac aggaagaagg
      241 ggaaaatgtt tcgtaaaaac agaaaattaa cagcgcacat caggaaacgc agtctcctga
      301 ttcagtgcta ccacatgaaa atttagttaa ctgttaccgt actaaaattt atgtcagaag
      361 atgttccatt accgaaagtc aatcagcgat acaaagacga tcatggtgcg cttgtcaccc
      421 tgaccagtgt tgaggaaacc cgggtggtgt ttatgcagga cggctatccg catccctgca
      481 tgcgaccgat gtacaacttt ctgggaaaat tcaaacccga accccgggaa gaaacagaat
      541 aaaacgaaat ccctgccggc aatcacacta cctgatagca gcataatcca cttattaatt
      601 ctcatccccg gagacaagcc cagaaaacca ctccgggtgt tcactgtatg ccgtgttcag
      661 cgcatccctg gaaacgttta tataaaagct gtcctgcaat ttgcgggtaa caacttctgc
      721 caggcgattc cggttatcca ccagtccggc ataacctctc tctgccagcc actcattcag
      781 ttcatgttcc agagacagat tgataatatc ataatcggac cttgccattc ccgtttcctt
      841 agcacatgtt ttcaggcacg gggataaagt ataaagaatg tcatactgct gctgccagag
      901 caatattccc cctgatggtt gtatcagaga tggtatgccg gtactttgcg ggttgttccc
      961 gtcaggttat cgtgcacctc cacggtcgtg gtcaccaccg gcattccggc ctttctcagc
     1021 ctcagaacat cagccgcaat acgctgactg ccaaaccaga acaggccgtc cagtgcagtc
     1081 accagtaaac ccgcctccag tgcatacttc agccgttcac ggggcgcttt cacttcccgg
     1141 gcaacctgct gatatggcaa tgatgtgttt tcattcccaa ccacgcgacg aatacggtga
     1201 gacagatgat accggtatgt atccggcaca ccagaaaggc tggccttcag gctgtacacg
     1261 cagccaaacc gtttatcgtt aaacaccaca tttttctggc tgatgcccca ttcttcacgc
     1321 agcgcggcaa tcaattgcgg tgtacgggta agcagcaagc gaaaaggcag ttcaaaactg
     1381 gtgacgtaat ccacattcag cagtgcaata cgcaaccgtt cttctggccc ggcctctgtc
     1441 tgccggcact cctccagaac atcctgccac tgccggcgaa gacgggggga ttcgttccgt
     1501 tctgtaaaac agtatttatc cgccagatag tcaattcgtg tacgcatact gaaaggaatt
     1561 ccgtataaag attcagcaga caaaacttta tcagccagta aaaattaacg gaagcattga
     1621 tattcctccc gacaatcacc ggatgattgt tgcaataacc tgcgacataa aggactgaac
     1681 agccgttttt aacgccacgt attgccctga tgaaccaggc cgggccaccc gaaaacatct
     1741 ttccacccgg cattgtccgc cagcacttat caccgaccag gtctgttgca gtaaatccac
     1801 cagttgcgca cattcttcat cagccatgac gccgactcgc ggatggcaaa tactcgtctg
     1861 gctgagcagc aacagacatc gtatctgtgc tccttcatgt tgttcccgca accagcgtaa
     1921 taccaccggc gctgatgggc tgaccgtgtg attatgctcc gtaatacgtt cgtgtacccg
     1981 tcggtgaaag gaactacaaa atgtctgaat ctgttgacca tagattcctt ctgccgaata
     2041 gagcgtgaag tgaaatgcca acagatttgg ccagtggtcc accgcctgct gaacaaaacg
     2101 ccggatttcc cccggctctg aaattaaggc ttcggttatt tgcacgaaat gttccttttc
     2161 aacccctgtt gaattttgtc aagccggtgc agacacagta aagcgcgcat caacacctga
     2221 aaggtacgat acaaacacaa gttacaaaca ataagaacgg aaggacaatt aatcagtgag
     2281 gaataataac taaattaacc aaaagaatgt tactgacaat a
//
LOCUS       DAZKGS010000331         2283 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKGS010000331 DAZKGS010000000
VERSION     DAZKGS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..362
                     /gene="tnpA"
                     /locus_tag="ACHOQY_005017"
     CDS             <1..362
                     /gene="tnpA"
                     /locus_tag="ACHOQY_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9680919.1"
                     /translation="PYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGG
                     LLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKL
                     TPALLQTLIREIKGSSH"
     gene            362..709
                     /gene="tnpB"
                     /locus_tag="ACHOQY_005018"
     CDS             362..709
                     /gene="tnpB"
                     /locus_tag="ACHOQY_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9680920.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            729..>2283
                     /locus_tag="ACHOQY_005019"
     CDS             729..>2283
                     /locus_tag="ACHOQY_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFP9680921.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKV"
BASE COUNT          552 a          628 c          653 g          450 t
ORIGIN      
        1 tcccttatga gtttaaaatc gccttagtgg agcagtcact gcagcccgga gcctgtgtgg
       61 cgcagatcgc ccgggaaaac ggaatcaacg ataacctgct cttcaactgg cgccatcaat
      121 accggaaagg tggcctgctg ccttccggaa aaaatatgcc ggcactgctt cccgtgacgt
      181 taacgccgga gccggataat aaaatcccgg cccccgcaca ggaaccagag cagataaata
      241 caccgtccga cagtctgtgt tgtgagctgg ttctgccggc cggaactctc aggcttaaag
      301 gtaaactgac gccggcgtta ttacagacac ttatccgcga aataaaaggg agcagccact
      361 gatgatatct ctccctgcag gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg
      421 aaatggcttt aacggcctgg catcaaaagt tcagaacgtc ctgaaggatg acccgttctc
      481 cggacacctg ttcatcttcc gcggacgccg gggtgaccag ataaaagtgt tgtgggctga
      541 cagtgacgga ctgtgcctct tcaccaaacg cctggagcgg ggccgcttcg tctggccagt
      601 cacccgtgac ggcaaggtgc accttactcc ggctcagtta tccatgcttc ttgaaggtat
      661 caactggaag cacccgaaac gaacggaacg cgctggaatc cgcatataac ccgttgtaaa
      721 gtgaggatat ggacacctca cttgctcatg agaacgcccg cctgcgggca ctgttgcaga
      781 cgcaacagga caccatccgc cagatggctg aatacaaccg cctgctctca cagcgggtgg
      841 cggcttatgc ttccgaaatc aaccggctga aggcgctggt tgcgaaactg caacgtatgc
      901 agttcggtaa aagctcagaa aaacttcgtg caaaaaccga acggcagata caggaagcac
      961 aggagcgaat cagcgcactt caggaagaaa tggcggaaac gctgggtgag caatatgacc
     1021 cggtactgcc atccgccctg cgccagtctt cagcccgtaa accgttaccg gcctcacttc
     1081 cccgtgaaac ccgggttatc cggccggaag aggaatgctg tcctgcctgt ggtggtgaac
     1141 tcagttctct gggatgtgat gtgtcagagc aactggagct tatcagcagc gcctttaagg
     1201 ttatcgaaac acaacgtccg aaacaggcct gttgccggtg cgaccatatc gtgcaggcac
     1261 cagtaccttc aaaacccatt gcacgcagtt atgccggagc ggggcttctg gcccatgttg
     1321 tcaccgggaa atatgcagac catctgccgt tataccgcca gtcagaaata taccgtcgtc
     1381 agggagtgga gctgagccgt gccacactgg ggcgctggac aggtgctgtt gctgaactgc
     1441 tggagccgct gtatgacgtc ctgcgccagt atgtgctgat gcccggtaaa gtccatgctg
     1501 atgatatccc cgtcccggtc caggagccgg gcagcggtaa aacccggaca gcccggctgt
     1561 gggtctacgt ccgtgatgac cgtaacgccg gttcacagat gcccccggcg gtctggttcg
     1621 cgtacagtcc ggaccggaaa ggtatccatc cacaaaatca cctggccggt tacagcggtg
     1681 tgcttcaggc cgatgcttac ggtggttacc gggcgttata cgaatccggc agaataacgg
     1741 aagccgcgtg tatggctcat gcccggagaa aaatccacga tgtgcatgca agagcgccca
     1801 cctacatcac cacggaagcc ctgcagcgta tcggtgaact gtatgccatc gaggcagagg
     1861 tccggggctg ttcagcagaa cagcgtctgg cggcaagaaa agccagagcc gcgccactga
     1921 tgcagtcact gtatgactgg atacagcaac agatgaaaac actgtcgcgt cactcagata
     1981 cggcaaaagc gttcgcatac ctgctgaaac agtgggatgc actgaacgtg tactgcagta
     2041 atggctgggt ggaaatcgac aacaacatcg cagagaacgc cttacgggga gtggccgtag
     2101 gccggaaaaa ctggatgttc gcgggttccg acagcggtgg tgaacatgcg gcggtgttgt
     2161 actcgctgat cggcacatgc cgtctgaaca atgtggagcc agaaaagtgg ctgcgttacg
     2221 tcattgaaca tatccaggac tggccggcaa accgggtacg cgatctgttg ccctggaaag
     2281 ttg
//
LOCUS       DAZKGS010000332         2268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKGS010000332 DAZKGS010000000
VERSION     DAZKGS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1238
                     /gene="escC"
                     /locus_tag="ACHOQY_005020"
     CDS             <1..1238
                     /gene="escC"
                     /locus_tag="ACHOQY_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP9680922.1"
                     /translation="KTNEISRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFN
                     SIEVRGVPECIKYITSLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVV
                     VPGVVSILKTMASNGSLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDI
                     YRQLISELDIEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANL
                     SSSVISNANNFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVA
                     SLESITSGTLLRVTPRILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSIL
                     PEVQNSEMTTEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKH
                     SVVRLFLIKATPIKSASSE"
     gene            1252..1707
                     /gene="cesD"
                     /locus_tag="ACHOQY_005021"
     CDS             1252..1707
                     /gene="cesD"
                     /locus_tag="ACHOQY_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP9680923.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(2093..>2268)
                     /locus_tag="ACHOQY_005022"
     CDS             complement(2093..>2268)
                     /locus_tag="ACHOQY_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFP9680924.1"
                     /translation="LHYKRLEIFIYDVNLEAVPIESPVSTGPKRKNYRVGNGIVGQSN
                     IWNEMIMRRKKEN"
BASE COUNT          750 a          439 c          428 g          651 t
ORIGIN      
        1 ataaaacaaa tgaaatatca agaagcataa ttacgccaac atatctggat attgactcac
       61 tgttaaaata tctgcgtgat acaatctcag taaataaaaa ctcctgtaac gtgagaaaaa
      121 tcacaacatt taactcgatt gaagttcgtg gcgttcctga atgcataaaa tacattacat
      181 ctttatctga aagtcttgat aaagaagcac agtctaaagc aaaaaataaa gatgtcgtta
      241 gagtatttaa attaaactat gcatcagcga cagatataac gtataaatac cgcgaccaaa
      301 atgttgtcgt gcctggcgtc gtctccatcc tgaagacgat ggcaagtaat ggctcacttc
      361 ccagcacagg caaaggtgct gtagaaagaa acggcaacct gtttgataac agtgtaaaga
      421 tttccgcaga tccacgtctt aatgcagttg ttgtaaaaga tcgtgaaata actatggaca
      481 tatatcggca attaatttct gagcttgata ttgagcaaag gcaaattgag atttctgttt
      541 caattattga tgttgatgca aatgatcttc agcaactggg agtaaactgg tccggtacat
      601 taaatgcagg gcaaggttct attgccttta acagctcaac ggcgcaagct aatctcagtt
      661 cttctgtgat aagcaacgcc aataatttca tgatcagagt gaatgcgtta caacagaact
      721 cgaaagcaaa aatactctct cagccatcaa ttataacctt aaataatatg caggcgattc
      781 ttgataaaaa cgtaacgttt tatacaaaag tttccggaga aaaggtggca tctctggaaa
      841 gtattaccag cggaacatta ctgagagtaa caccacgtat acttgatgac aactcgaata
      901 gtcttaccgg aaaacgtcgg gaacgagttc gtttgttact tgatattcag gatggcaatc
      961 aatcgactaa ccaaagtaat gctcaggatg ccagcagcat cttacctgaa gtacaaaatt
     1021 cagaaatgac aacagaagcg acattaagcg ctggagaaag tctgctgctt ggcggattta
     1081 tccaggataa agagagttca agtaaagatg gtataccgtt gttaagtgat attcctgtta
     1141 ttggtaactt attctccagt acaacaaaac aaaaacacag cgttgttcga ctctttttga
     1201 ttaaagcgac cccgataaaa tcagcatcta gcgaataata aatgaggctt tatgagcagg
     1261 aaatttagct ctctacagga tatctatgat ttctaccagg atggtggtac gttagcatca
     1321 ctgacaaatt tgacgcaaca agatctcaat gatcttcatt cttatgccta tacagcatat
     1381 caatctggtg atgttataac cgcaagaaat ctatttcatc tgctaacata tctggagcac
     1441 tggaattatg actacaccct atctctgggg ttgtgtcatc agcgtttatc aaatcatgaa
     1501 gatgcacaac tgtgtttcgc tcgctgtgca actttagtta tgcaagatcc cagggcatct
     1561 tattattctg ggattagcta cttattggtc ggaaatcgga aaatggccaa aaaagccttt
     1621 aaggcttgtt taatgtggtg taatgaacaa gaaaaatacg ctacatataa agaaaatatt
     1681 aaaaaattgt taggtaatac agattaaaaa ctaccatctg ccataccaat ccccaggctg
     1741 tctggggatt ttctatcgga aataataaag ctgctttggt gaagtgcgcc caacgttgag
     1801 caccgaccgc tggcagattt atctacagga gtgggttact ccattaacca ggattttatg
     1861 cgcctgaacc atcttctgct gtactcaatg tcatttccgc ttatagaata agcagtatat
     1921 atcttaactt agcatcagaa atctgactgt ttcaatcatc tatagcccag agtgtatttg
     1981 tcaggaaatg actttcaaac tttggcagag aaaaaggctt atcctggaaa acaaagccct
     2041 tgaatagagc tttattttta ttcttctata aaatatactc aaaaaaatac gtttaattct
     2101 cctttttccg cctcatgatc atttcgttcc agatattaga ctgccccaca ataccattac
     2161 cgactcggta gttttttctt tttggtccgg ttgataccgg actttctatc ggaacagcct
     2221 cgaggttaac atcataaatg aaaatctcaa ggcgcttata atgcaagt
//
LOCUS       DAZKGS010000333         2263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKGS010000333 DAZKGS010000000
VERSION     DAZKGS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..321
                     /locus_tag="ACHOQY_005023"
     CDS             <1..321
                     /locus_tag="ACHOQY_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9680925.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..879
                     /locus_tag="ACHOQY_005024"
     CDS             487..879
                     /locus_tag="ACHOQY_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9680926.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSLPRTAQQLS"
     gene            complement(889..1857)
                     /locus_tag="ACHOQY_005025"
     CDS             complement(889..1857)
                     /locus_tag="ACHOQY_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9680927.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
     gene            2043..>2263
                     /locus_tag="ACHOQY_005026"
     CDS             2043..>2263
                     /locus_tag="ACHOQY_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9680928.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPA"
BASE COUNT          533 a          601 c          589 g          540 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgcagacaat gtcaccacga ttattatggg gagcggtact gcacacactg ccagaccgga
      841 ggtttcagtc ttccccggac agctcagcaa ctgtcttgaa cgtaaaaatt atggagcaac
      901 gtgatggtac gattcattcc attcttcgtt ctttctgagc attgcgttca ggatagtcag
      961 cagcttgcgt atacaggtaa ccagcgccac ttttttgggt tttcctgcgg caagcagtcg
     1021 catgtaaaac acctttatta ccgggttaaa gcgggttgcg acaagcgctg ccatgtacag
     1081 ggctgttcgc acaccagccc tgccgccaaa gatggttcgt cgccctctca tcgtgcccga
     1141 gtcccggttt actggaacca taccgacaag cacactgatt tcacgcctgg agaggttacc
     1201 ctgttcgggt acttccgcca gcagcgcagc agccgtcatg gtgccaacac ctttaatact
     1261 gctcagtcgc ttagcaagct ctttgaagtg gctatggata tggctgttca tgtcagcatc
     1321 tagtcgcgca agctcgtctt ccagtgcttt gatgatgata ttaatgcttt tcctgttcaa
     1381 tggatgagca gggtgcaacc ggtttctctc ggcaaccagc atcgtaatca actgccgcct
     1441 acgaactacc attgcagtga gaacctgaca ttcagtatcc ggcagtgctc tgataaagcg
     1501 ttcccgctcc ggatgccggt tgagaacctc agccatctgg gaaagcactc tggcatcaat
     1561 actatcagtt ttcgcgaggt atcccatggc gcgggcaaag tcacgagctt gtctggggtt
     1621 aaccacggca agctcaaaac cttctgcctg aagcgcacag actatggcgg tttcaagtcc
     1681 tccagtggct ttcattaaaa ccagcgcaat ggcgtacatt ctcaactcag caatgatggc
     1741 gtcaaagcca tcactgtcat tgccgacaga aaattgttca atagcgctgc tggcggcaat
     1801 atccagtatc gctttcgaaa catcgatatc cacacaaagt gaacctggct gactcatgat
     1861 tacccatcct tgcaaatgcg ttatggcgta ctgacaactg ttcgggtttc agatgagtgg
     1921 ctcagtgtat gcgccattcg ctgagctacg ggctatgaac cccttgaggg atcgggctgc
     1981 atacaccttg ccaaatctaa tcacagttat tttaaccaat tgtcaagata caaggaggag
     2041 atatgcgaat tctgaactgc tatatggcga atgacagcaa aggccatttt gttacggcga
     2101 aagaagccgc gaagcacaac cgacaggacg ttttgtgctg tgtgtcctgt ggttgtccgt
     2161 taacacttaa gcggggaaat gacggacaac cgccgtggtt tgaacatgac cagatgactg
     2221 ttgctgcgaa aatcctgctg cgatgcacct ggcttgaccc ggc
//
LOCUS       DAZKGS010000334         2258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKGS010000334 DAZKGS010000000
VERSION     DAZKGS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..266
                     /locus_tag="ACHOQY_005027"
     CDS             <1..266
                     /locus_tag="ACHOQY_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFP9680929.1"
                     /translation="RDCFVEMLQDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTV
                     YDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            294..1252
                     /locus_tag="ACHOQY_005028"
                     /pseudo
     CDS             294..1252
                     /locus_tag="ACHOQY_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1264..1725
                     /gene="yibG"
                     /locus_tag="ACHOQY_005029"
     CDS             1264..1725
                     /gene="yibG"
                     /locus_tag="ACHOQY_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9680930.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          697 a          407 c          533 g          621 t
ORIGIN      
        1 ttagggattg ttttgtggag atgcttcagg ataaaaatga agaagtccgt attgaagcaa
       61 taatcggact ttcctacaga aaagataaaa gggttttatc tgttttatgc gatgagttaa
      121 aaaaaaatac tgtttatgat gatatcattg aagctgcggg tgaattaggt gataaaacgc
      181 tacttcctgt tttagatact atgttgtaca agtttgatga caatgaaatt ataacttccg
      241 ctattgataa gctgaagcgt tcatagcgtg agttgcctat gcacagtggg ggattcccgc
      301 cggcacggtg cagatgctcg accggctgga aagtgaaatc ctggctgacc gggtgagtga
      361 ggaaagccgc cgctggctgg catcgtgcgg cctgaccgtg gagcagatga aaaaccagat
      421 ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct
      481 gccgctggcg ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat atgatgaatg
      541 gggcaacctg ctgagtgatg agaacccgca tcatctgcag cagctcattc gcctgccggg
      601 tcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct
      661 gcaggggcgg tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca
      721 gtatccgttg aatccggtca taaatgtaga tccgcaaggt ttggttgata taaatttata
      781 ccccgaaagt gatcttatcc attctgtagc tgatgagatt aatatcccag gcgttttcac
      841 aatcgggggg catggtaccc ccacatctat tgaatccgca acgcgcagta tcatgacagc
      901 taaagatcta gcatatctaa ttaaatttga tgggaattat aaagatggga tgacagtttg
      961 gttattttct tgtaatacag gtaaaggaca aaattcattt gctagccaat tagctaaaga
     1021 gttacataca aatgtaatag gacctgacac gctatggacg tggtgggggc gaggaactaa
     1081 tggtaagtta aaaatggata cagtgctaac agcaccaacg aaccttaatt caaataagga
     1141 tctaatggct ataacaacaa aagaccttgg taattggata acatatgggc catctgggca
     1201 ccccatttct aatatgcaag gtacgccaga aaaacccagt gatataagat aggttgtaga
     1261 tgtatgaaag catgcttgtt actatttttt tatttttctc ttattggtca attgcatggt
     1321 gctgatgtga aaataaaaga aaacgaaagt gtgatgggat ctacagcaat gacctatgat
     1381 ttaagtgaag aaaagctgat gaaactgaag tataaatcac aacatggtga ttcagaggca
     1441 tcatttcgac tatatcaata ttactgcttt actaaaaata atattgataa acaattgcga
     1501 ttcttggaaa gatcagcatc tcaggggaat gttacagcgc agtttaatta tggggtcttt
     1561 ttatcagata caaatccaac attatcagaa tattataatt tgaatagagc tatttattgg
     1621 atggaattcg ctgtaaataa cggtaatatt gatgcaaaga gcaaacttca agagctcaaa
     1681 aagctaaagc gaatggatag aaggaagaat aaggagaatc cgtaaaagca atagttcatc
     1741 agactgccgc agcagtagta tgatgatgaa tcagggctgt actataacta ttatcggtaa
     1801 cattaaccgc agcaggggcg gtataacact caggctccga ttgggctgga aggtaggtgg
     1861 aatttttatc agtatccgtt gaacccgatt tctggaatcg atccccttgg gattagccac
     1921 ctgtttatac agtataactt taggtatgct ttcgtgtgtt tcagatatac catgtgatga
     1981 taatttttat gatgtgctta gtattccagt cgcttctggt aataatatgc aatgtaaaaa
     2041 taatccccgt tgtacacatt tataaataga tggccaatac ctcaaggcct atggagttag
     2101 aatgttaatg acccgggagc aagcaataga aaacctgacg gcatacgatt agtaccgtca
     2161 gttaatacag gaacttataa tcgcaatgga ttttcaatac atagttgttt aaacgctttt
     2221 ggtccgtctt taggacctcg attctgttcc gagggttg
//
LOCUS       DAZKGS010000335         2227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKGS010000335 DAZKGS010000000
VERSION     DAZKGS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2227
                     /locus_tag="ACHOQY_005030"
     CDS             <1..>2227
                     /locus_tag="ACHOQY_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9680931.1"
                     /translation="TAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTGLKISGAS
                     TAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGK
                     LTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASGATKTLSG
                     VLNGVAGNIDNVATAAGALVAVGVARYFGNMAAGVGKATTELIAAKSGTVALAAAQAD
                     AAVQVQRKAAADALAAERAWQLAQAELALARNSSAEALATQNAVAKRRAMIVANAALV
                     QSNQAVAASRKALENATSVTGMLKSGAASLLSLVGGIPGLLMLGAGAWYTVYQNQEQA
                     RESARQYASTIDEIRAKVPGMTLPELADNEGKTREALEEQNRLIAEQESKVRGLKTQI
                     ADYQRWLQENPQGGSGVEIITHGLAEATGKLAVEQSRLTQMQEKAKSVQEALASLEYN
                     RVARIREEAAEQNRAYQALLQMNVQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATL
                     DDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEA
                     WRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELK
                     TLTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERAR
                     ERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAE
                     SD"
BASE COUNT          558 a          519 c          753 g          397 t
ORIGIN      
        1 acggcatttt cggataatgc ggccctgttt gcccgttcgg ctgcctcgat gcgtgaatat
       61 ggttacagtg ccggtgatgt actgaaggtg acggaggcca tttcgacagg gctgaaaata
      121 tccggtgcca gtacggcaga ggcgggctcg gtgatcaccc agttcagcca ggcgctggca
      181 cagggtgtgt tacggggtga ggaatttaat tcggtcaatg aaagcggaga ccggatcata
      241 cgtgcactgg ctgcgggtat gggcgtggcc cgtaaagacc ttaaggcgat ggcggacgac
      301 ggcaaactga cggcggataa agtcgttcct gcgttaatca gccagctggg ggtattgcgt
      361 gatgaatatg ccgccatgcc ggaaacggtc tctgacggga tcacaaaggt ggaaaacgcc
      421 tttatggcct gggtgggtgg cgcgaatgaa gccagcggag cgacgaaaac gctctccggc
      481 gtgctgaacg gtgttgccgg taatattgat aatgtggcaa cagccgcggg ggcgctggtt
      541 gccgtcgggg tggcccggta ctttggcaat atggccgcag gcgtgggaaa ggccaccaca
      601 gagctgatcg cggcgaagtc cgggacggtt gcccttgcag ccgcacaggc agatgcggct
      661 gttcaggtgc agcgtaaggc ggcagctgac gcgctggcgg cagaacgtgc ctggcaactg
      721 gcacaggctg aacttgcact ggccagaaat agcagtgcgg aggcgctggc cacacagaat
      781 gccgtcgcaa aacgccgggc catgattgtg gccaatgccg cactggtaca gtcgaatcag
      841 gcggtggcgg cctcgaggaa ggcgctggag aatgccacgt cggttacggg aatgctgaaa
      901 agtggcgcgg catccctgct ttcgcttgtc ggcgggatac ccggtttact gatgctggga
      961 gccggtgcct ggtacacggt gtaccagaat caggaacagg ccagggagtc cgcccgtcag
     1021 tatgcatcga cgattgatga aatccgggca aaggtaccgg gaatgaccct tccggaactg
     1081 gctgataatg agggtaaaac tcgcgaggca ctggaagagc agaatcgtct gattgctgag
     1141 caggaaagta aagtccgggg actgaaaaca cagattgctg actatcagcg ctggctgcag
     1201 gaaaatccac agggtggttc aggtgtggag attataaccc acggccttgc ggaggcaact
     1261 ggcaaactgg cggttgaaca gtcacgactg acacagatgc aggaaaaggc gaagtctgta
     1321 caggaagctc tggcgtcact ggaatacaac cgtgtggcga ggatacgcga ggaggcagca
     1381 gagcagaaca gagcatacca ggcattgctg cagatgaatg ttcagcatac ggaattcaac
     1441 cgtctgctgg ggctgggtaa tgaactgctt cagcagcggc agggactggt gaatgtgccg
     1501 ttacggctgc cacaggccac cctggatgat aaacagcaga gtgccctgac aaaaacagag
     1561 cgtgagctgg ccctgtccag actgaaaggg gaagaaaaag agcgtgtccg gctggggtat
     1621 gcggcggatg acctcggttt tgtgggtgat ccgtatcagg aggcgagaca acgttatatc
     1681 agtaatgccc tggaagcctg gcgcaataac gaggcgaata aacccaaatc ccggggtgga
     1741 aaatcagaga cggaaaaagc ggaagacagt ttttcccggc tgctgaagca gcagaaagag
     1801 caactggcac tggtggggca gaatacagag ctggcgaagc tgaaatacca gactgcgcag
     1861 ggcgaactga aaaccctgac ggagatgcag aagcaggagc tgctgcgtaa tgcggccctg
     1921 attgaccagc aaaaaatccg ggaacagttg cgatcccggg aagagacact gaagaatgag
     1981 aatgcggctg cgcgtgcgtc gaatgatgct gaactgctgg ggtacgggca gggggaacga
     2041 gccagagaac gcatgcggga gttgcagcag atccgcgaca gcttccgcca gaaggatgcg
     2101 gaccttcagt ctcagtatca gaccggggat atcagtgagg atttttacag acaggctctg
     2161 gcgcagaatg cacagtatct gagtgaacgt ctgaaagagc aggaagcctt ttatgccgaa
     2221 tcggatg
//
LOCUS       DAZKGS010000336         2194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKGS010000336 DAZKGS010000000
VERSION     DAZKGS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            341..1093
                     /locus_tag="ACHOQY_005031"
     CDS             341..1093
                     /locus_tag="ACHOQY_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFP9680932.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            1090..1644
                     /locus_tag="ACHOQY_005032"
     CDS             1090..1644
                     /locus_tag="ACHOQY_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFP9680933.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1646..1918
                     /locus_tag="ACHOQY_005033"
     CDS             1646..1918
                     /locus_tag="ACHOQY_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9680934.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          647 a          517 c          528 g          502 t
ORIGIN      
        1 gtgaggagaa aagatccagt aataaactga aataaatatc gcgacttata tgaaaaagaa
       61 atatatgttt aattttatct ctgtgaacag tcatgaacag tagatgatca ctttaattaa
      121 aacactacac cacttaacca catgtattta ctatattttt attgaagtga acagtagtga
      181 atagtatgta ttaaaggaag cgtgtagatc agacatgaca cctttctctg gctagccaga
      241 acaaggtcat tgttcagtca ctgacacaat cctcctcggt agtgcgcttg tatggacccc
      301 ggcacaattg acacaacaac aaaacactac cggagcagtc atgactactg ttaaccagat
      361 ccctgatgca aacattacac cctccctccc accgaaaatt cgcgaagcgg tggataaagt
      421 taaggcagca aaagctgtct ggcaggaaga acgacgaaaa caaactgaag ctgctgcaat
      481 gattgaaact atccgtaaac gtcaggaaga tacaaaaacg gaaacgcagg cgcttaatga
      541 tgaatggcga aacctgtttc gtgagaatca gggaaatatg acgccacgca tgaaaaaact
      601 gcgggcagaa atcgccctcg gacgcgaaac actggatgag ttcgaagatt tgctggcagc
      661 tcaggcggca gaaaatgaat tcctgccctg gaaaactgcg gatgctgcaa accactacat
      721 cagcgaacat aatcgcctga ttgaaactca tgcagtgtgg ctctggaatg agtttatgaa
      781 ggaacacggt cagaaactta ttcagatcct tggactgctg aaaatgactc tgggccgaag
      841 cgcttctgct gttatcggtg tagttcatac cgtaaacgac cccgaaagtg tgctgaagca
      901 atttatcagc gagcaactca ccactccggc actgtcctgt aacgtatctt caaccgatga
      961 tattaccctg ccggggatca gcatttatgc ggacgataaa gccatccagg atgccagaca
     1021 atcccccagc cctgcagcgc gttcacggat gcttaaacag cgtgacatgg ttaaaggggg
     1081 cgagaagaaa tgaatgccgg aaccattact caggaagcac tcaacgatta ccgctcggcg
     1141 ataaaaagct ggctgacact acgtaatacg caaagtacct accagcttcg tctggcggct
     1201 ctgctggata ctgaagaaaa accggcagca tatgccagcc agcttgagga tcttcgtgag
     1261 cgtcttgcag tcctcgaatg gcagattaac tgtgctgcac gggatggtct ttatgctcat
     1321 cagattgtgc tggagagttg tgttacaagc gcaacggaaa acttcatgag cgagcatggt
     1381 gatgcactca ctgacgctct ggctcctttt ctttgcgctc catacgggct tgaggcggca
     1441 atgaaaatat tacgaaccgc tgtagcccga caaacggaaa tgcgtactcc ggtaatttca
     1501 gcagcatata agagcattat cgacgaaacc ggattaacgg tggatgcatc aatgcgcgct
     1561 gatgcttcag ccagtttcac cccggcacaa cataaagttt tcctggcccg ccttaaccga
     1621 cttaatgaaa aaggagtgtg ctgatatggc cctgaagtgt cctgaatgtg gcgcggtcgc
     1681 tcacgccaga accagcgcct atgaagctcc gtcggttaaa cgctcatggt atcagtgcca
     1741 gaatctggaa tgttcctgca catttaccgc actggaaagc gtggatacga taattatgaa
     1801 gcctcggtgc aatgaacagg aatcagacaa agcaaaaata ccggaaaaac aacagcaaac
     1861 tctcaatcgc tatggctccg cgtcaaagct gtcaaaccgt cagcaaattc ctgtctgatt
     1921 agcaaaatac gaccacgaag tcccggtcca gtaccgggat tttttacgcc tttttcctgg
     1981 ctggcctgag agcgtatgag tgcatatcta tggcgcatga aaacgcatga gtctcatgcg
     2041 ccatttttga cgcgaaagcc cttgtgtggt ggcttctgag acgatttacg gggtgcatga
     2101 aaaccagtct gttaagcgaa gcgggcaggc gggcggggca ttgcgcgcag gaatgtgtat
     2161 accgttttag taatgatcgc tgacatctca cttt
//
LOCUS       DAZKGS010000337         2185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKGS010000337 DAZKGS010000000
VERSION     DAZKGS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..290
                     /locus_tag="ACHOQY_005034"
     CDS             189..290
                     /locus_tag="ACHOQY_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680935.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            287..742
                     /gene="ybcN"
                     /locus_tag="ACHOQY_005035"
     CDS             287..742
                     /gene="ybcN"
                     /locus_tag="ACHOQY_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP9680936.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            742..912
                     /locus_tag="ACHOQY_005036"
     CDS             742..912
                     /locus_tag="ACHOQY_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9680937.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            905..1195
                     /locus_tag="ACHOQY_005037"
     CDS             905..1195
                     /locus_tag="ACHOQY_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9680938.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1192..1554
                     /gene="rusA"
                     /locus_tag="ACHOQY_005038"
     CDS             1192..1554
                     /gene="rusA"
                     /locus_tag="ACHOQY_005038"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9680939.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1551..1691
                     /locus_tag="ACHOQY_005039"
     CDS             1551..1691
                     /locus_tag="ACHOQY_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9680940.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1777..2160
                     /gene="quuD"
                     /locus_tag="ACHOQY_005040"
     CDS             1777..2160
                     /gene="quuD"
                     /locus_tag="ACHOQY_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFP9680941.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          576 a          489 c          553 g          567 t
ORIGIN      
        1 gcgtttctaa ttctatggta aatggtttag aaatcgttga ctgtctgatc tatgagttaa
       61 aataagaaat ctcttgctgg gtatcattat tgtttaaatg acttttgatt ttcaataatc
      121 aacttgtcat aattaagtca ccggagtttg aactcctccg gtgacttctg cgctaaacgg
      181 ggacgtttat gcgcacatac aatctaaact atcttctccc gtcacagatg cagaaatgca
      241 cctgcgattt tctttatcca gcgtttgacc tctgcggagg tgaagcgtga acctcccaca
      301 agacggcatc aaactgcatc gcggtaactt caccgctatc ggtcagcagc tccagcctta
      361 tttggaagac ggcaagtgct ttcgcatggt gcttaaaccg tggcgcgaga gacgcagtct
      421 ttcccagaat gcacttagcc acatgtggta cagcgaaatc agtgaatacc ttatccgcag
      481 ggggaaatcg ttcgctaccg cagcatgggt aaaagatgct ctcaaacaca catacctcgg
      541 ttatgaaacc aaggacctgg ttgatgtcgt aaccggcgaa atcactacta tccagtcgtt
      601 acgccatacc tccgatcttg ataccggaga gatgtatgtc ttcctgtgta aggttgaagc
      661 ctgggcgatg aatattggct gccacctgac tattccgcag agctgcgagt tccagctgct
      721 gcgcgacaag caggaggcgt aatggctaca ccgcttattc gtgtcatgaa cggacacatc
      781 tacagagtac caaatcgtcg taagcgtaag cctgagctaa aaccatccga aataccaaca
      841 ctgctcggat ataccgccag cctggttgat aaaaaatggt tgcgactggc agcaaggagg
      901 aatcatggct gatttgagaa aagcagcgcg tggtcgcgaa tgccaggtaa gaatccctgg
      961 cgtatgcaat ggcaaccctg aaacgtctgt actggcacat atccggctgg ctggactgtg
     1021 tggcaccggt attaaaccgc cagaccttat tgccaccatt gcctgttctg cctgtcacga
     1081 cgaaatcgac cgccgcacgc attttgtcga tgctgagtac gcaaaagaat gcgcgctgga
     1141 aggtatggcg agaacgcagg ttatctggct gaaagagggg gtaatcaagg cgtgaatacc
     1201 tacagcatca cattaccctg gcctccgagc aataatcgct attaccgcca taatcgcggg
     1261 cgcacgcacg tcagcgcaga ggggcaggca taccgcgata acgtcgcccg aatcattaaa
     1321 aacgcaatgc tggatatcgg cttggctatg cctgtgaaaa tccgcattga gtgccacatg
     1381 ccggatcgcc gtcgccgtga cctggataat ctgcaaaaag ccgcttttga cgcactcact
     1441 aaagcaggtt tctggctgga tgatgctcag gtcgttgatt accgcgttgt gaagatgcct
     1501 gttaccaaag gtgggaggct ggaactgacc atcaccgaaa tggggaatga atgatgtttg
     1561 agttttatat ggcagaacgt cttcgccacc gctgggggcg tctgcgctta tatcgtttcc
     1621 ccggttctgt tttgaccgat taccgaatac tgaagaatta cgccaaaacc ctgacaggag
     1681 caggagtatg aagtcagaga taacaatcaa ctaatactgt ttcgttgatt tttgcttgta
     1741 attggcgttc tggtctgatt tttgtggagt aagttgatgc gtgatattca gatggttctt
     1801 gagcgttggg gagcgtgggc ggctaataat catgaagatg tgacctggtc gtccattgcc
     1861 gccggtttta agggattaat tacttcaaaa gtaaaatctc gcccgcaatg ttgtgacgat
     1921 gacgcgatga tcatttgcgg gtgcatggcc cgtctgaaaa agaacaacag cgatttgcac
     1981 gatttattag tagattatta tgtagtcggt atgacattca tgtcactggc aggtaagcat
     2041 tgctgctctg atggttatat cgggaaaagg ttacagaagg ctgagggcat aattgaaggg
     2101 atgttaatgg cattagatat ccggttagag atggatatcg ttgttaataa ctctaattaa
     2161 tatgccaatt gtttactaaa aatta
//
LOCUS       DAZKGS010000338         2181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKGS010000338 DAZKGS010000000
VERSION     DAZKGS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..1758
                     /gene="yfbK"
                     /locus_tag="ACHOQY_005041"
     CDS             4..1758
                     /gene="yfbK"
                     /locus_tag="ACHOQY_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFP9680942.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAA
                     QQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKA
                     ASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAV
                     RVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKS
                     EELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALP
                     SISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNV
                     GIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQ
                     KVLNSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIG
                     AGKHITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLV
                     EFPLGPTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYR
                     AEFIRLIELADGVTDISQ"
     gene            complement(1829..>2181)
                     /locus_tag="ACHOQY_005042"
     CDS             complement(1829..>2181)
                     /locus_tag="ACHOQY_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFP9680943.1"
                     /translation="NIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
BASE COUNT          633 a          467 c          522 g          559 t
ORIGIN      
        1 tatatgcgaa ataaaaatat aatcatgttg cttatgagta gtttgatttt gtcaggatgc
       61 gggccggaat ctgagaataa ggaaagtcag caacaacagc ccagtacccc cacagaccag
      121 caagtgcttg ccgcgcaaca agctgcaata aaagaggccg agcaaagatc cgtcgccgat
      181 aaagccactg cagacgcgaa agcaaaagcc ttagcccagc aagaagcgca acaatattca
      241 gacaaacagg ctttgcaggg gcgcttacag gcggctccaa aatatcaaca tgcagccaga
      301 gaaaaagcag cgtcccaaat cgcaaatcca ggaaccgctc gctacaagca gttcgatgat
      361 aatccggtta agcaggtagc gcaaaacccg ttggcgacgt ttagccttga cgtcgacacc
      421 ggcagttatg cgaatgtacg ccgttttctc aatcatgggc tgttacctcc gccagacgct
      481 gtgcgggtgg aggagatagt caattatttc ccgtatgact gggatatcaa agacaaacaa
      541 tctattccgg ccactaagcc aatacctttc gctatgcgct acgaactggc acctgcgccg
      601 tggaatgaac agctaacatt gctgaaaatt gatatcctgg cgaaagatca taaaagtgaa
      661 gagttaccag cttctaatct ggtctttctt atcgacactt ctggttcaat gatttctgat
      721 gaacgtttgc cacttatcca gtcttcgttg aaattattgg tcaaagaact tcgtgagcag
      781 gataacattg ccatcgtgac ctacgctggc gactcccgta ttgcgttgcc ttctatctcc
      841 ggtagtcaca aggcggaaat taatgccgca attgattcgc tggatgccga aggcagtacc
      901 aatggcggtg ccgggctgga actggcttat cagcaggcgg cgaaggggtt tattaagggc
      961 ggcatcaatc gcattttatt agccactgac ggtgacttta acgttggcat tgacgatcca
     1021 aaatcgattg aatcaatggt caaaaaacag cgggagtctg gtgttactct gtcgacgttt
     1081 ggcgtgggga atagcaatta caacgaggca atgatggtgc gaattgccga tgttggtaac
     1141 ggcaactaca gctacattga taccctctct gaagcgcaga aagtattgaa tagtgaaatg
     1201 cggcagacgt tgattaccgt agcaaaagat gtcaaagcgc aaattgagtt taaccccgcg
     1261 tgggtaacgg aataccgtca gattggttat gaaaagcgcc aacttcgggc ggaagatttt
     1321 aataacgaca acgttgatgc gggggatatt ggcgcaggca aacatataac gttgttattc
     1381 gaattaacgc tgaacgggca aaaagcatca attgataagt tacgctatgc cccggataac
     1441 aaatcagtga aatcggacaa aacgaaagaa ctggcctggt taaagattcg ctggaaatac
     1501 ccgcagggaa aagaaagtca gttagttgaa ttcccgctgg ggccaacaat aaacgccccc
     1561 tctgaagata tgcgtttttg cgcagcagta gctgcatatg ggcaaaagtt acgcggttct
     1621 gaatacctga acaatacctc ctggcagcag atcaaacagt gggctcagca ggcaaaaggg
     1681 gaagatccac agggttacag ggcggaattt attcgcctga ttgaactggc ggatggtgtg
     1741 actgacatca gtcagtgatg actgtttagc aaactatgtt cgaccagtca gcatatttgc
     1801 tgactggtcg aattgattaa caatgatgtt aactcactct tttgccggat gctctatttt
     1861 tgggttgtga gtgttttatt tctaattgtg tataagaagc attgattcgt gcatcgccat
     1921 atttatctaa ataacaattt ctgtttacca gtcgatgttg aaaatcaatt tcgtactgct
     1981 cttctgctga atatttttta aatctgtcag catagttttt taataacgta tagggggcgc
     2041 tgtcagaatt gctttccctt tgttcaatca cttcctgggc tgccatgcac acaaacgcac
     2101 cgcaactttg gcttaagtat tgctgaagat cggcatgtaa aaatattatc tctggttcat
     2161 ttaaatgaac ttttatatta t
//
LOCUS       DAZKGS010000339         2177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKGS010000339 DAZKGS010000000
VERSION     DAZKGS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..1277
                     /locus_tag="ACHOQY_005043"
                     /pseudo
     CDS             300..1277
                     /locus_tag="ACHOQY_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1800..>2177
                     /locus_tag="ACHOQY_005044"
     CDS             1800..>2177
                     /locus_tag="ACHOQY_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680944.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDG"
BASE COUNT          665 a          472 c          487 g          553 t
ORIGIN      
        1 tttgatcaca actgaagatg ctttttacaa tgaagaatta ttgttaaaaa acgctttgtt
       61 gaatcaaccg aactgttgct gagttgcagg atcacatcac gtattttcct gaaaacgcag
      121 gctgcttagt agcaaagcag aagttcaaaa tcaccaacta aatagctgcg ccgaatagta
      181 gatcaatgaa agggaactca gcccggattg tgcgatctga tcaatcgcca aaaatccccc
      241 cctaaccgga ctgagcgatg ccgatcatag caccaattcc ccgcgacgaa tgacgcctga
      301 tgcaaaaagc aatctataaa actcacgata aaaattatgc ccgcaggctg accgccatgc
      361 tgatgctgca ccggagcgct cgcgtcagtg acgttgccag aacgctctgc tgcgcccgtt
      421 cctccgtcag gcgctggatt aactggttca cgctgtcgag tgttgaaggt ctgaaatcat
      481 tacccgcagg acgttcccgc cgctggccgt ttgagcatat ctgcacattg tttcgtgaac
      541 tgataaaaca ctctcccgga tattttggct atcagcgttc acgctggagt acagaactgc
      601 tggcaataaa aatcaacgag ataaccgagt gccagttaca tgccggaacc gttcgccgct
      661 ggttgccatt agtcgggctt gtgtggcgca gggctgcgcc aaccctgcgt acccgtaacc
      721 cgcataaagc agcaattcac aaagcactgg acgaatgtag tgcggagcat ccggtctttt
      781 atgaagatga agtgtatatc tatcttaatc ccaaaaccgg tgcggactgg caacttcgca
      841 gacagcaaaa acatgtggta acaccggggc agaatgaaaa atactatctg gctggtgctt
      901 tacatagtgg aacgggcaaa gtcagctatg tgggtggcaa cagcaaaagc tcagccttat
      961 ttatcagtct gttgaaacat ctgaaattga cataccgacg ggataaacct atcacgctca
     1021 tagtggacaa ctacatcatc cataaaagtc gggaaacaca gcgctggctg aaagaaaatc
     1081 cgaagttcag agtgatttat cagccagttt actcgccgtg ggtgaatcat gttgagcggt
     1141 tatagcaggc gcttcacgat acgataacgc gcaatcatca gtgccgttca atgtggtaac
     1201 tgcttaaaaa ggttcattat tttatggaaa ccgtgagccc attccccggt ggtaaatatg
     1261 ggctgttaaa agtgtagcgg tattcggcgc agctatttag gatggtcagg tcgcagaagt
     1321 tctgtgccac tacagtgcgt gtactaaaca aaatgacgag ggcacgaatg tcataatacg
     1381 tgcgtattac ctgcagtagc taccaactga gggggcttat cccaaacctg atttattcaa
     1441 caaagctgga ctggacttat ccttactctg aattattttc cagacatatg catcatgtac
     1501 tggctcgctt ggttcaggaa atacaggagc aaatctacag ctattagatt tattcctatt
     1561 tgtatgcaaa gtcgcattgc ataatgcgga gctatgttac agatacgcct gacgtgcgta
     1621 gtaaaattat aaagcaacat atatatacaa ggagaaaccg agaaaaataa atgagtcgta
     1681 tgttgggtag catcgagata aatatttttt tatcacatta gcaccttggt taagggtgtt
     1741 aataataaga ttttctcact tattaaaaca taaataattt tttaagcgga aatgtaatta
     1801 tgagactgcc agagaaagtt ctttttcctc ctgtcactag tggcctgtca gggcaggaaa
     1861 aacaaaaaaa accgaagagc attaccggat ttcaggaaaa ttatcaacgc aatatcaggc
     1921 caatcaaaac agcatcagaa gcccgactac gcttctttga taaaatggtt tcgaaagaaa
     1981 actctctgga agatgttgtt tctttaggtg aaatgattca gaaggaaatt tatgggcatg
     2041 aacaaagaac attttcacca gttcatcata caggtaactg gaaatcatca ttgttacaca
     2101 acgcgctcct tggtctggca aatgtttata atggcttacg ggaaacagaa taccctaaca
     2161 ctttcaacag agatggt
//
LOCUS       DAZKGS010000340         2167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKGS010000340 DAZKGS010000000
VERSION     DAZKGS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..506
                     /locus_tag="ACHOQY_005045"
     CDS             <1..506
                     /locus_tag="ACHOQY_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680945.1"
                     /translation="NIFSELWGVGLFVTLIVLNIVFNLFFRLLFILSKMILMSRHIRK
                     MQKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNIINTDLVIGEKIHFRT
                     LSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDNHNTPWLL
                     NNENVQE"
     gene            complement(1377..1517)
                     /locus_tag="ACHOQY_005046"
     CDS             complement(1377..1517)
                     /locus_tag="ACHOQY_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9680946.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1819..2082)
                     /locus_tag="ACHOQY_005047"
     CDS             complement(1819..2082)
                     /locus_tag="ACHOQY_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9680947.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
BASE COUNT          678 a          490 c          389 g          610 t
ORIGIN      
        1 acaatatatt ttcagaacta tggggagttg gcctgtttgt tactctgata gtactgaata
       61 tagtatttaa tctctttttt agactcttat tcattctctc gaaaatgata ttaatgtcac
      121 gacatataag aaagatgcaa aaaaaactaa aacagacgtc tgtgcctgat tttatttatt
      181 attcaataga aaaacacaac aaatatccca tatatgtatt acacatgcca tcaaacaata
      241 taattgaaat aggctacaat ataataaaca ccgatctggt cattggcgaa aaaattcatt
      301 tcaggacact aagcaatcgt tctttatatt ttaacctcac ccaaccccca ttaatcgcca
      361 caataaaaga tgatgtgttt tgcactcttc atgattacta taaccacaat aatgaaacga
      421 aatccactat tgacaattac atatcaaaaa taaaggataa tcataatacc ccatggttat
      481 taaacaacga aaatgttcag gaataaaaaa aagagacaat tcccgagata aaacaaaaga
      541 gcataaacat atgaaccgga aattctattt caaaaatatc ctgtggggaa tgtgccccat
      601 tataatatcc atattgcatc tgatatatga cacaccaaca gaagcatcga taaaagttga
      661 cttatttctg atactcagtg gcctggccct tccttgttca atatacctga caaaaacaat
      721 cacactaaaa tacacatcca tatcattctg gaaaaaacct cttttctggt cttatggtat
      781 atatcatttt catcttgttt atataatgac atgtatagtt ttagcaatac cttctgcaat
      841 tatcgtttta ttctttcact tttatcaaaa catcaccaga aactggaagt gacaaccgca
      901 ataatgaaca taaaaaggca aatcagaatg gcagtaaatc aataaagtac catttcatat
      961 gaatcatcag aaaaacggtg gacgcgacat ccagcacgag gcaaaacaca gcaacaaaat
     1021 aagccggata tcgcgccctg tacttttcta actcaactcc cggcattgtg caagttttaa
     1081 tcgcaggtgg attcagagcc acgcccccat tgattcagcc tgaagacacg tatatccttg
     1141 gagaaagcag gaatctcatt gctgtgaaat tccttacaga atcttcagcc ccattttatg
     1201 gggctttttt tggtgccccc acgctgttac cacactttcg ggatgcgaat aaaaaagccc
     1261 acagttttta cactgcggac ttttgttttt tctttgtgat ttgtgtgcaa gggtgcgact
     1321 ttcgaccgaa cctttggcaa ccggatagcg gggactgttg tccccgctgc ggtttcttac
     1381 ggtttacact gcaagaacgc cgcaatttcc gaccacatga agcttaccct gaattcacac
     1441 ggtgatgcgt gtaacaccca gacggtcagc aacatggtca ggcataacat gcccacaatg
     1501 atcgtttttt gcggcatagc acttgctcct tttacagaga ggcgctaacc ttccacttgc
     1561 tgaaggtaga tggtcagggc ctcgggttaa cgtttaagtt gactcggggc ctttccacat
     1621 ccggccttca ggtaatcctt ccagccgtca gccgaaaggc acccgcgcgt aatgtactgg
     1681 ttttcagaga ggtccgcaat cttccggtga cgagatcatc tgcacacgcc ccggtgggac
     1741 gggctaacgg tatattcagg tgcaggatct ctctgtctgg tgcgttttta aattgtcaaa
     1801 cgcaccctgg cggcaaaatc agccaccttt tttcgcatca acaaaacgcc caaaacgctg
     1861 ggtaaagcgt gcaacattac cttctgaagc gacagttctc agtttacctg tataaaacgg
     1921 atgcgactta gaagaaacgt caattgtaac atatgggtaa gttacaccat ccagttcaat
     1981 tacccggtca gttttgatcg tcgaaccgat cctgaagtac tcatcaatac tggtatcgtg
     2041 aaatactact gtacggtatt ccggatggat attcggtttc acaacagttt tcccccttta
     2101 agtgaaaatt ccgcataaag gtacatattc agacggtcag attcaactgt ctgttcttat
     2161 aacctca
//
LOCUS       DAZKGS010000341         2127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKGS010000341 DAZKGS010000000
VERSION     DAZKGS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..150
                     /locus_tag="ACHOQY_005048"
     CDS             <1..150
                     /locus_tag="ACHOQY_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9680948.1"
                     /translation="DENGIIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWEN
                     IGGMK"
     gene            256..1137
                     /gene="nleH"
                     /locus_tag="ACHOQY_005049"
     CDS             256..1137
                     /gene="nleH"
                     /locus_tag="ACHOQY_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFP9680949.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            1361..>2127
                     /gene="cif"
                     /locus_tag="ACHOQY_005050"
     CDS             1361..>2127
                     /gene="cif"
                     /locus_tag="ACHOQY_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFP9680950.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKH"
BASE COUNT          647 a          369 c          513 g          598 t
ORIGIN      
        1 gatgagaatg gcattattcc ggagcaggac agggtaatca cacaggtggt gatactggat
       61 acagataaaa agctgataca gtgtgtggta agaccgctgc aaatcctgcg tgctgacggg
      121 acgtgggaaa atattggcgg gatgaagtaa cccgacagct tcacaaaacc ggagtccggc
      181 tccggttttt gttgtcatgt catggtgatg tttgttaaga aagtaaagat tgattcattt
      241 tgaaggttga aatgtatgct atcaccatct tctgtaaatt tggggtgttc atggaattct
      301 ttaaccagaa acctgacttc tcctgacagt cgtattttat cctctgtaag ggatgcagct
      361 gctagctcgg ataatggggc gcaagtaaag gtgggcaaca gaacatatcg tgttgttgtc
      421 actgataata agttttgcgt tacaagagaa agtcatagtg gttgctttac taatctgttg
      481 cacaggctgg gatggcctaa gggagagatt agcaggaaaa ttgaggtgat gctgaattca
      541 tcgccagtga atagggctat ggaaagaggt gctgttcatt cgaatagacc tgatttacct
      601 cctgttgatt atgcaccgcc agagttaccg agtgtggact ataacagctt gcctgtgcct
      661 ggtaatgtta ttggcaaagg gggtaacgct gtagtatatg aagatgctga ggatgcaaca
      721 aaagtcctga agatgtttac tacatctcaa agcaatgaag aggtaacaaa cgaagttcgt
      781 tgcttcaacc aatattatgg tgccgggagt gcagaaaaaa tatatggcga taatggcgat
      841 attattggta ttagaatgga taaaataaat ggagaatcgc ttttaaatat ttcgtccttg
      901 ccggcacagg ctgagcatgc catttatgat atgtttgaca ggcttgagca aaaaggaatt
      961 ctttttatcg atacaacaga gacaaatgtc ttatatgatc gcacgagaaa tgaatttaat
     1021 ccaatagata tatcatctta taatatttct gaacgttcat ggagtgaaaa tcaaataatg
     1081 caatcttatc atggaggaaa gcaagatctt attagtgtgg tattaagtaa aatttagtaa
     1141 ttttatccag tgtagtggat ttgttgcatg gatggaggtg gtaaaaagtg gtgtggctgg
     1201 caatccaggc cgcgtcacag aaatggacaa tgccgctgag agactggcga atggcataca
     1261 ggcaccagaa aaatttcaga catgacataa tgcccatgcc agaagttctt tttgcacctc
     1321 gcctgtattt atagataaat atagagtgaa tgaatgaggt atgaaagaca ttacccttcc
     1381 ccccccgacg tccgcgtcct gtctgacagg ggccatatct gtaaatactg aagctgtatt
     1441 atctcccatg caacacactt cagccttaca tgtaagagat tttgcttccc tgtgctcaca
     1501 gaacctcaaa gctaatgtat tgctaaatag tgatgaccac gaagtaccta tacatcagaa
     1561 aaatcctgct gcaataatgc aaaatatcga ctctaacatc aaacagatgg caacagactg
     1621 ggggatgtcg attgaggagg ttgaggttat tatagggcga gagaaaggca ttgtggaacc
     1681 ctcctgcgga gttaccgcta atgctattat gaaactattt ctggacaagg atggcttcag
     1741 ttactgcttt gaaaatgaac agacactatc gctcgagcag cttcaggagc gcctgtcctg
     1801 tatgcctgaa tgtaagagct ttgtattacg tgttaatgat ggtgcgcttg gtcatgctta
     1861 cattgtcgat attcccaaag gagaaaactc ttgtcgtcct gcattcttgt atcagtcaga
     1921 tttaggagag ggcgtcacca gaaagttaag atttgaggac tggatgacgc ataaagcatt
     1981 gactccgatt ttgctggatg atatttgtaa ttacttctcc tgcatgtctc aaaataagac
     2041 agatttggag cagattgcaa cgttatttga tattgatgga aatgttaaaa tgttacgaaa
     2101 ggaaaatatt caatatcaaa agcatga
//
LOCUS       DAZKGS010000342         2124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKGS010000342 DAZKGS010000000
VERSION     DAZKGS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..628
                     /locus_tag="ACHOQY_005051"
     CDS             <1..628
                     /locus_tag="ACHOQY_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9680951.1"
                     /translation="IHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGL
                     PFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFG
                     EGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQN
                     GTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            749..>2124
                     /locus_tag="ACHOQY_005052"
     CDS             749..>2124
                     /locus_tag="ACHOQY_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFP9680952.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAA"
BASE COUNT          505 a          552 c          570 g          497 t
ORIGIN      
        1 gatacacaac gcctccggac tgtgggctga cattgtggcc ctgggaaccc gccacagcat
       61 gaaagcgtca acggacaata acgacttccg cgcccggggt tggggctggc tgggttcact
      121 ggaaaccggt ctgcccttca gtatcactga caacctgatg ctggagccac aactgcagta
      181 tacctggcag ggactttccc tggatgacgg taaggacaac gccggttatg tgaagttcgg
      241 gcatggcagt gcacaacatg tgcgtgccgg tttccgtctg ggcagccaca acgatatgac
      301 ctttggcgaa ggcacctcat cccgtgcccc cctgcgtgac agtgcaaaac acagtgtgag
      361 tgaattaccg gtgaactggt gggtacagcc ttctgttatc cgcaccttca gctcccgggg
      421 agatatgcgt gtggggactt ccactgcagg cagcgggatg acgttctctc cctcacagaa
      481 tggcacatca ctggacctgc aggccggact ggaagcccgt gtccgggaaa atatcaccct
      541 gggcgttcag gccggttatg cccacagcgt cagcggcagc agcgctgaag ggtataacgg
      601 tcaggccaca ctgaatgtga ccttctgaca gaaccatcgc ctctctgtgg tcccggtcat
      661 catgaccggg acccggaccg gcgcaacgga tcttcaacgc cacattcgct ggcattaaca
      721 ataacatgat attcatcacg gagtgactat gttacagata gtcggcgcgc tgatcctgct
      781 gatcgcagga tttgccattc ttcgcctttt gttcagagca ttaatcagca cggcttctgc
      841 gctggcaggg ctcatattgc tgtgtctgtt cggcccggcc ttactggctg gctatatcac
      901 cgaacgcata acccggttgt tccatattcg ctggctggca ggcgtatttc tgacgattgc
      961 cggaatgatc atcagcttca tgtggggact tgatggtaaa catatcgcgc tggaggctca
     1021 cacctttgac tctgtgaaat ttattctgac caccgctctc gccggtggtc tgctggctgt
     1081 tcccctgcag atcaaaaaca ttcagcagaa cgggatcaca ccagaagata tcagcaagga
     1141 aattaacggg tattactgct gtttttatac tgcctttttc cttatggcgt gttctgcatg
     1201 cgcaccattg atcgcgttac agtacgatat ttcaccgtca ctgatgtggt ggggcgggtt
     1261 gttgtactgg ctggctgcat tagtgacgct gctatgggcg gccagccaga tccaggcgct
     1321 gaaaaaactg acctgtgcca tcagccagac actggaagaa caaccggtgc tcaacagtaa
     1381 atcgtggctg accagtttgc aaaacgatta cagccttcct gactcactga cggagcgcat
     1441 ctggctgacg ctcatttctc aacggatttc ccggggagag ctgagggaat ttgaactggc
     1501 agacggaaac tggttactga acaatgcctg gtatgaaaga aacatggcag ggtttaacga
     1561 acagttgaaa gagaacctgt cattcacacc tgatgaactg aaaacgctct tccggaaccg
     1621 cctgaattta tcaccggaag cgaatgacga ttttctcgat cgttgcctgg acggcggtga
     1681 ctggtatccc ttttcagaag gtcgccgttt tgtatcattc catcacgtgg atgagcttcg
     1741 tatctgtgcc tcctgcgggc tgacagaagt acatcatgcc ccggaaaatc ataagccgga
     1801 tccggaatgg tactgctcct ctctttgtcg cgaaacagaa acactgtgtc aggaaattta
     1861 tgaacgccct tacaacagct ttatttccga tgcaacggcg aatggtctga ttctcatgaa
     1921 actgccggaa acctggagta caaatgagaa aatgtttgct tccggagggc agggacatgg
     1981 gtttgccgct gaacggggaa accatattgt cgacagagtc cgtctgaaaa acgcacggat
     2041 cctcggtgat aataatgcca gaaatggagc agacaggctg gtcagcggaa cagaaatcca
     2101 gacgaaatat tgttcaactg cagc
//
LOCUS       DAZKGS010000343         2123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKGS010000343 DAZKGS010000000
VERSION     DAZKGS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..1371
                     /locus_tag="ACHOQY_005053"
     CDS             106..1371
                     /locus_tag="ACHOQY_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9680953.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYANVNNPLRR"
     gene            1662..2015
                     /locus_tag="ACHOQY_005054"
                     /pseudo
     CDS             1662..2015
                     /locus_tag="ACHOQY_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          587 a          505 c          504 g          527 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtactcaa aagcctcgag
      481 acacacgttt tcccctttat cggcaaccgg gatatcacaa cactcaatac cccggacctg
      541 cttatccctg ttcgtgctgc agaagcaaaa caaatttatg aaatcgccag tcgtctgcag
      601 caaagaatat ctgctgtaat gcgttacgcc gtacagtctg gcatcatcag atataacccg
      661 gctctggata tggctggtgc attgaccacg gtaaaacgcc agcatcgccc cgctctgaat
      721 ctttcacgcc tgcctgaact tctgtcgcgt attgacggtt ataaaggcca gcctgtcacc
      781 cggcttgccg ttatgctgaa tttactggtt tttattcgtt ccagtgaact cagatatgcc
      841 cgctggtctg aaattgatat tgacaatgcc atgtggacta ttccagccga acgcgaacct
      901 ctgccaggcg taaaattctc acaccggggc tccaagatgc gaacaccaca tcttgtgcca
      961 ctcagcaaac aggctgtagc catactgaca gaactacaga catgggctgg tgaaaatggt
     1021 ctgatcttta cgggagcaca tgacccgcgt aaaccaatca gtgaaaatac tgtaaataaa
     1081 gccctgaggg tgatggggta tgacacaacc caggatgtct gtggccatgg attccgggcg
     1141 atggcgtgca gtgcattgat tgaatcaggt ttgtggtccc gcgatgctgt ggaacgtcag
     1201 atgagccatc aggagcgtaa tggtgtacgt gctgcgtata tccataaagc agaacatctg
     1261 gaagaacgtc gactgatgct acagtggtgg gccgattttc ttgatgcgaa ccgggagaag
     1321 gggatcagtc cgtttgaata cgcaaatgtt aacaatccgc tgagaaggtg acagaaggct
     1381 attcacacac atgctcttcc catggggaag agcaacctgc tccaataaat ttctttttta
     1441 ttccggctga tagcaccgca atcacccgtt acctctaaat agccacttag cattcggcgt
     1501 ataagttgac agagaaggag tatctcagca ggagcttaaa gtgtggtttt aggcagaatt
     1561 tgattgatcc tccatttggg gcatgtgact aatcgatata gcttcctgaa cggaaaaagt
     1621 gttttccctt ccaatataaa ctattcgaca ataatataaa tcaattacag aataaaactc
     1681 tgtataaaca tgcaaaaatg gtaaaggcgt tcgactatat cctgaatcac tggaatgcgc
     1741 tgaacgagtt ctgtcgtgac ggctgggtgg aaatagacaa caacatcggt gaaaacgcgt
     1801 tacgatcggt ggcggttgga agaaaaaatt atctcttctt cggctcagac aaggggggag
     1861 aaagcgcggc gatcatctac agtctgctgg tcacctgcaa actgaacgaa gtggagccgg
     1921 aggactggtt gcgcgaagtg atcgtgaagc tcaatgactg gccgtcgaac cgtgtgcatg
     1981 aactgctgcc ctggaactgc tcaactgtaa aataatcctt acgctacgta cttctcgggg
     2041 cgcttacgaa tttttcacgt gccacagaat tttgaacgct ttctcttaca atatttcaat
     2101 gtttctatca gtattcgccg gaa
//
LOCUS       DAZKGS010000344         2115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKGS010000344 DAZKGS010000000
VERSION     DAZKGS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            778..1755
                     /locus_tag="ACHOQY_005055"
     CDS             778..1755
                     /locus_tag="ACHOQY_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9680954.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            1939..>2115
                     /locus_tag="ACHOQY_005056"
     CDS             1939..>2115
                     /locus_tag="ACHOQY_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9680955.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          569 a          520 c          504 g          522 t
ORIGIN      
        1 gtctccagga ttcccggggc ggttcagtgg ataaatgctt attacaacgt ttatcgacct
       61 catacaaaca atggtggttt atcgccttgc cgcatgaaga aaaatggaaa caggttattc
      121 cggtgtcctg attttatgat ccactacaca ttcctgacga acgccactgc ataaatcaca
      181 ttgcacatcc tcagagagta cgcagtatag gaggggtata tatacattat cggtatatca
      241 ggcgacttct gtctgtatgt tgtcggtgct cgccgcagac cggtgaccgg gcgaagcccg
      301 cgaaccggcg aggaagcgca atgtatctgt agttacagtg agcagatacg ccctgaagct
      361 tcctcataaa acagttgtgt aaactgtttt cccggaatga tttatccaca tatccggcgg
      421 cccgatccag taaacagatc cataaatgat caagaaatga tccattaaag atccccatat
      481 cgctgcaaac cttgtcactc atgctccggg accacgatca cataagctgt ggcatgtttc
      541 tgataaactg tagcaggatc atgataagct gtattcagta atgtatatac tgtagtaagt
      601 taatgacata aattatggtc agtacgccag gctcagctgt taaatacaga ctgcaggttt
      661 ttcttcagtc agttgacggg gttctgacac tcaattttct gtttattctt ttactgtcca
      721 caggcaggag gctttctgga aaacgaaaat tcagacatca aaaaactgtt cggcgaggtg
      781 gataagtcgt ccggtgagct ggtgacactg acaccaaaca ataacaacac cgtacaacct
      841 gtggcgctga tgcgtctggg cgtttttgta ccgaccctta aatcactgaa gaacagtaaa
      901 aaaaatacac tgtcacgtac tgatgccacg gaagagctga cacgtctttc cctggcccgt
      961 gctgagggat ttgataaggt tgagatcacc ggcccccgcc tggatatgga taacgatttc
     1021 aagacctggg tgggaatcat tcattccttt gcccgccata acgtaattgg tgacaaagtt
     1081 gaactgcctt ttgtcgagtt tgcaaaactg tgtggtatac cttcaagcca gtcatcccgc
     1141 aggctgcgtg agcgcatcag cccttccctg aaacgcattg ccggtaccgt gatctccttt
     1201 tcccgcaccg atgagaagca cacccgggaa tatatcaccc atctggtaca gtcagcctac
     1261 tacgatactg aacgggatat tgttcagtta caggctgatc cccgcctttt tgaactgtac
     1321 cagtttgacc ggaaagtgct gctgcagctc aaggccatca atgccctgaa gcgacgggag
     1381 tccgcccagg cactttacac ctttatagag agcctgcccc gggatccggc accgatatcg
     1441 ctggcgcggc tgcgtgcccg cctcaatctg aagtctcctg tattttccca gaaccagacg
     1501 gtcagacggg caatggagca gttgcgcgag attggatatc ttgattacac ggagatccag
     1561 cgggggcgga caaaattctt ctgtattcac taccggcgtc cccggttaaa agcgccgaat
     1621 gatgagagta aggaaaatcc gttgccacct tcacctgcgg aaaaagtcag tccggagatg
     1681 gcggagaaac ttgccctgct tgaaaaactg ggcatcacgc tggatgacct ggaaaaactc
     1741 ttcaaatccc gctgaacata aactgtagtc agtgaagagt gctcctttac tgactacagc
     1801 ttatattatc aggtgcagtg agtggtctgc tcactgcagt ttatatgtaa gcgtacagcc
     1861 tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg
     1921 ggaaccaaag tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct
     1981 aattatcctc ccgaatttaa acagcagctc gttgctgcct cctgtgaacc cgggatatcc
     2041 atctcaaaac ttgctcttga aaatggcatt aacgccaatc tgttgttcaa atggcgacaa
     2101 caatggcgcg aggga
//
LOCUS       DAZKGS010000345         2104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKGS010000345 DAZKGS010000000
VERSION     DAZKGS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..356)
                     /locus_tag="ACHOQY_005057"
     CDS             complement(<1..356)
                     /locus_tag="ACHOQY_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9680956.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            484..1344
                     /locus_tag="ACHOQY_005058"
     CDS             484..1344
                     /locus_tag="ACHOQY_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9680957.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
     gene            1833..>2104
                     /locus_tag="ACHOQY_005059"
     CDS             1833..>2104
                     /locus_tag="ACHOQY_005059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680958.1"
                     /translation="MSSSGQSLALFYHDVGVFISSTRLSISTPYAAFFVTSVSISTML
                     THDVEPVIDTLKSVRRLFSNQVTASCLRLSAGVIEELPVNDGDIMT"
BASE COUNT          561 a          436 c          525 g          582 t
ORIGIN      
        1 ggaaccagac ggacttcatc ttttggcggg cgtccgcgtc gcgcgcgcac tttttcggac
       61 aggatccgca caaacggtct tacaccatcc agatcaaatg attccggtgt cagcatccgg
      121 gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg gtgacgctga
      181 tccggcatat agaaaagaag ggcactcact tccggggcca gcaggtaggc cggtgtggcc
      241 cctgtggaag tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca
      301 atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt
      361 tatttttcac gtagcacacg cactgtttat ggatactgat tgcgctgggc attctggtat
      421 tgtgactggt atgatgctgg cgctgattct gatgattaat aaacaacata acacaggata
      481 tttatgaaaa ttacggatca caaactttct gaaggtattg ctctgacatt tcgtgttcct
      541 gaaggaaata tgaaacatcc tttgattatc ttatgccatg gattttgcgg tatccggaat
      601 gttctgctcc cttgctttgc gaatgctttc acagaagcag ggttcgcaac gattactttt
      661 gattatcgtg gattcggtga gagtgaagga gaacggggcc gtctggttcc agcaatgcag
      721 atcgaagata ttatttcagt tatcaattgg gcagaaaagc aggcatgtat tgataatcag
      781 cgtatcgggt tatggggaac atcgctgggt ggttgtcatg tgttcagtgc agctgcgcaa
      841 gatcaaagag tgaaatgtat tgtcagccag ctggcttttg ctgatggtga tgctctgatt
      901 accggagaga tgaatgaatt ggagagagct tcttttctgt ccacactgaa taaaatggca
      961 gagaaaaaga aaaacacagg gaaggagatg tttgtggggg ttacacgggt actaaatgat
     1021 gatgagtcga aggtattctt cgagaaagtc aaagctcagc atcccgaaat ggatataaaa
     1081 atacctttcc ttactgtcat ggaaacgttg caatataaac cggccgagag cgcagccaga
     1141 gtccagtgtc cagtattggt tgttattgct ggccaggata gtgttaatcc accggaacag
     1201 ggaagagcat tatatgatgc tgttgcttct ggtactaaag aattatatga ggaagctgac
     1261 gcatgccatt atgatattta tgagggcaca ttttttgagc gggtagcagc tgtgcaaaca
     1321 caatggttta aaaaatatat ttaatagtga gcctgtgttt accattctgt gtaaatactt
     1381 tttcttaaaa gtgaccgtca aggcggtcac taaactttat aataaagagg catcgttcat
     1441 ttccgggagg cagactggat tgccagcccc ccacttttta ccaagctgca ggtatggggg
     1501 acacggacac tggtgttcac agaaaaaaat aaccatctct gaaatgtctg aaccgccccg
     1561 gttttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt ttctgatgga
     1621 agcataataa gctttttctg cttctgccgg agggatatga cccagccttc aggaaaaccg
     1681 gggcggttca aaccactttt gcaccgatcg tgacattttc ccttaataaa gcatctgtaa
     1741 ctgaaacaat cagcatatta ataatatatt gacaggcatc attgctgtct gtgaaaaata
     1801 agtatctaca aacatgcaaa gccttttagc cagtgtcttc ttcaggtcag tcgctggccc
     1861 ttttttatca tgatgtaggt gtatttatct ccagcaccag actttctatc tcaacgccat
     1921 acgctgcatt ttttgtaaca tccgtcagca tcagcactat gctgacgcat gacgtggagc
     1981 ctgtgattga cacgctgaag tcagtcagaa ggttgttcag taatcaggtg accgcctcct
     2041 gcctgcgcct gtctgctgga gtcatagaag aattacctgt taacgacggc gatatcatga
     2101 catt
//
LOCUS       DAZKGS010000346         2085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKGS010000346 DAZKGS010000000
VERSION     DAZKGS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHOQY_005060"
     CDS             <1..480
                     /locus_tag="ACHOQY_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9680959.1"
                     /translation="LNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYVGA"
     gene            480..806
                     /locus_tag="ACHOQY_005061"
     CDS             480..806
                     /locus_tag="ACHOQY_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP9680960.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVAAMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTTVKDDDSEAAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            803..1192
                     /locus_tag="ACHOQY_005062"
     CDS             803..1192
                     /locus_tag="ACHOQY_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9680961.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDRQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            1212..2009
                     /locus_tag="ACHOQY_005063"
     CDS             1212..2009
                     /locus_tag="ACHOQY_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9680962.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          511 a          513 c          609 g          452 t
ORIGIN      
        1 ttaaatgacg ttcagaaatt cattgaagcc catccggggt gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt ctgcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggagccgga accacaacca gttcgtgaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc ccgggtgatt ttgtgcctga cccgccaggc ggaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcagtgttt acggaaaagc
      421 agcaagcgcg ctgtatcggg tgatgagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgacgacgtt aactcaatgc cagcagcagg tgctggatat gctgatttct tatcagaaag
      541 aacgtggttt cccgccaacc aatcaggagg tggcagccat gctgggatac cgttcggtga
      601 atgcagcggt ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg
      661 gcgtggcccg gggtatcacg cttcatacca cagtgaagga cgacgacagc gaggcagccg
      721 ggattatccg cgcactgctt gccggtgagg aaaacgccag actgcgtgca gcccactggt
      781 tacatgagag gggcctgaaa gtatgaagct gatcctgcct ttcccgccca gcgtgaacac
      841 gtactggcga caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg
      901 gcgcaaattc cagagcgcgg catgcgcagc aatagttgag cagttacgtc gtctgccgaa
      961 accaacgtcg gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg
     1021 cgatctggac aactataaca aggcgctgtt tgacgccctg acccacgcgg gtgtgtggga
     1081 agacgacaga caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa
     1141 ggtcgagatc actatcagta agtatgagaa accggcgggt gcagccgcct gattaagagg
     1201 agaaacgaag tatgaataat ctgatggtta ttgatggtat tgaagttcgt cgtgatgctt
     1261 atgggcgtta cagcctgaac gatctgcaca gggctgctgg ttctctggat aagcataagc
     1321 ctgcattctg gctccgcaat gagcaaactg agcgtttaat aagcgagttg cagatttgca
     1381 actcggtcaa tatagagcca gttaacgtta ttcgtggcgg aaataaccag gggacgtatg
     1441 tctgcaaaga actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg
     1501 tgatccgtac tttcgacatg gtaaccagcg caccggaaaa attatccgga caggctgctg
     1561 acaagatgca ggctggtgtg atcctgctgg actttatgcg ccgggaatta aacctgtcta
     1621 actcatcagt gcttggagcc tgtcagaagc ttcaggaggc tgttggctta ccgaatctgg
     1681 caccgcgcta tgccattgat gctcctgctg acgcgcctga tggctcaagc cgccccacgc
     1741 tgtcactgag tgcactgctg aagcagtatg gtatccgcct gacagctaat caggcatatc
     1801 accagatggt gaagctgggg atcgtcgagc agcgcgaacg atacagccgt accgcgatta
     1861 acaacatcaa aaaattctgg tcgctgacag cgaaaggctg catgttcggc aagaatatca
     1921 ccagtcccgc aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc
     1981 tgttaaagct gctcgatacc gttcattgag gtgaccgtga gagcactact gacccctgaa
     2041 attgccccgc gtatggggat cgtattgttc aggccaggtt cagag
//
LOCUS       DAZKGS010000347         2077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKGS010000347 DAZKGS010000000
VERSION     DAZKGS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1733
                     /locus_tag="ACHOQY_005064"
     CDS             <1..1733
                     /locus_tag="ACHOQY_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9680963.1"
                     /translation="TRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLAS
                     AQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKA
                     GELAARDRAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYN
                     TLMAAAKKDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKI
                     SQQRRDLWKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQK
                     RLNELAQQAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKA
                     TSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAM
                     LTGSEQSWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAA
                     AANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVG
                     GAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMR
                     DGGVFSGGRR"
     gene            1730..2059
                     /locus_tag="ACHOQY_005065"
     CDS             1730..2059
                     /locus_tag="ACHOQY_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9680964.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     EFKQVVN"
BASE COUNT          494 a          494 c          720 g          369 t
ORIGIN      
        1 agacccgtcg cctgaaagaa aacatgggaa cactggagac ctgggcggat aaaacaggga
       61 aggcattcaa atcgatgtgg gatgccattc tggatatcgg tcgtccggaa tcctcagcgg
      121 atatgctcgc cagtgcgcag aaggcatttg atgaggcgga taaaaaatgg cagtggtacc
      181 agagtcggag ccagcgccgc ggtaaaacct cctctttccg ggccaacctt cagggcgcat
      241 ggaatgaccg ggaaaatgcc cgtctggggc tggcagcggc cacgctgcag tcggatatgg
      301 aaaaagccgg tgaactggcc gccagggacc gggccgaacg ggacgcatca cagctgaagt
      361 ataccggaga ggcgcagaag gcgtatgagc gtctgctgac gccgctggag aaatataccg
      421 cccgtcagga agaactgaat aaggccctga aagacgggaa aatcctgcag gcggattaca
      481 acacgctgat ggcggcggcg aaaaaggatt atgaatcgac gctgaaaaag ccgaagtcgt
      541 caggagtcaa agtgtcagcc ggtgagcgtc aggaagacca ggcgcatgct gccctgctgg
      601 cgcttgaaac cgagctccgg acgctggaaa aacacagcgg tgcgaatggg aaaatcagcc
      661 agcagcgtcg cgatttatgg aaagcggaaa atcagtatgc ggtcctgaaa gaggcagcca
      721 cgaaacggca gttatctgag caggaaaaat ccctgctgac ccatgagaaa gagacgctgg
      781 agtacaaacg ccagctggct gagctgggag acaaagttga acaccagaaa cggctgaatg
      841 agctggcaca gcaggctgcg cggtttgagc agcagcaggg cgcgaagcag gcggcaatca
      901 gtgcccaggc gcggggcctc accgaccgtc aggtgcagcg ggagtcggaa gagcagcgcc
      961 ttcgtgacgt gtacggtgat aatccggatg cgctggcgaa ggccacatct gcactgaaga
     1021 acacctggtc tgcggaggag cagcttcgtg gaagctggat ggccggtctg aagtccggct
     1081 ggggcgagtg ggcagaaagt gcgacggaca gtttttcgca ggttaaaagc gtggccacgc
     1141 agacctttga cggtattgca cagaatatgg cagcgatgct gaccggcagc gaacagagct
     1201 ggcgtggttt cacccgttct gtgctctcca tgctgacaga gatttttctg aagcaggcca
     1261 tggtggggat tgtcgggagt attggcagcg ccatgggtgg tgctttcggt ggtggggcgt
     1321 ctgcctccac ggggacggcc attcaggctg cggcggcgaa cttccatttc gcgaccggag
     1381 gatttacggg aaccggtggc aaatacgaac ctgccggtat tgtccaccgc ggggagtttg
     1441 tcttcacgaa ggaggcaacc agccggattg gcgtcggcaa cctgtatcgt ctgatgcgcg
     1501 ggtatgcgga aggtggttat gtgggcggtg ccggaagtcc ggcgcagatg cggcgggcgg
     1561 aaggcattag ttttaatcag aacaatcacg tggtgattca gaacgacggc accaacggac
     1621 aggcggggcc gcagctgatg aaggcggtgt atgacatggc ccgcaagggg gcgcaggatg
     1681 agattcaggc gcagatgcgt gatggcggcg tcttttccgg aggcaggcga tgaaaacatt
     1741 tcgctggaaa gtgaagccgg atatggaggt gaactcgcag ccatcggtgc gtgaagtgcg
     1801 ttttggtgac gggtattcgc agcgtatggc ggcggggctg aatgctgacc tgaaaacata
     1861 ccgtgtgacg ctttccgtga cccgggagga ggcccgacat ctggaggcat tcctggcaga
     1921 gcacggtggc tggaaggcgt ttctgtggac accgccttat gcctggcggc agataaaggt
     1981 gacctgtgcc gcctggtcat cacgggttcg catgctgcgg gttgaattca gtgccgagtt
     2041 taagcaggtg gtgaactgat gcaggatatt cacgaag
//
LOCUS       DAZKGS010000348         2050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKGS010000348 DAZKGS010000000
VERSION     DAZKGS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..275
                     /locus_tag="ACHOQY_005066"
     CDS             6..275
                     /locus_tag="ACHOQY_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9680965.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(392..667)
                     /locus_tag="ACHOQY_005067"
     CDS             complement(392..667)
                     /locus_tag="ACHOQY_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP9680966.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(730..>2050)
                     /gene="espK"
                     /locus_tag="ACHOQY_005068"
     CDS             complement(730..>2050)
                     /gene="espK"
                     /locus_tag="ACHOQY_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFP9680967.1"
                     /translation="SYSAETSAGIKSEITPIQIEEARASGRLYIKDCDIEYLPQLPNE
                     ITSVTIENCNNLTILTGLPVNTQNLSVINCEKLQITDIPSTVKNLHIELTDSPFIHII
                     PEGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSLSINSYNPENQARID
                     NLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIGVEGMPDGLDLDLQN
                     VLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVNSIKLVNDFSKKDII
                     TQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSHNNYNVTMADLSVYN
                     RDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDGYGQSITASELRWIY
                     RNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATYKYS"
BASE COUNT          476 a          389 c          487 g          698 t
ORIGIN      
        1 atgacatgaa catactaaaa aaacttatgc agcgtatgtg tggttgcgga aagcatgatg
       61 gccgtgaaca cgtgcagtcg cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtccgatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatt ctgcgtggtg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaactcga cagcttcaca aaaccggagt
      301 ccggctccat tttaccacct ccacgggagg tggttgcttg caatcagatg tttcgggcct
      361 gcggcttttt gtgttcccta gtaagtggtt actattcttt tatgttattc ctctctgtat
      421 ctattgtctt agatagccat atgagttcag atgctgagag ttttaattgt ggtgttctgt
      481 ctcctgctcc gggtggacgc atgtcaaaag tgcaggattt tctgctgaat actgtgtagt
      541 cattcataga gcatgttgtt gaggttagtg tacaattctt cgatactggc ttgtcctgca
      601 caggtccgga tatttctggg tagcttcctt ttgaatttga aaatatgttt ttgattgaaa
      661 atggcatcgt gctttgtcct ttgaactacg atatattgtt ttgtgttctt tcgttgtgag
      721 taggtatcat taagaatact tatatgtggc tccagagtat ttgggtttgt agtctttcca
      781 ctctggtgcg cctaatattt tttctgctgg tatctctttc ccacgcaagt aaaactttat
      841 gtttttcatt atctggtcgt tgtctttgtt gcgataaatc catcgtaatt cgctggcagt
      901 aatactctga ccataaccat ctgacttatt agcaatgagt ttgagtgagt tgacaatctc
      961 gtcagcacaa aaaataaccg ttttgttgcg tgttaatgtc tgaaactcca ggccggcctt
     1021 gcttgttttt gcccataatt tgtcgcgatt gtatacggaa aggtcggcca tggtgacatt
     1081 ataattattg tggctcttta ggtgttgttt aaattttatc cctctttcgt gttcatttaa
     1141 tgctcttttg atcagtggat cttgcttata tttgcgaggt gttctggggt cccatactgc
     1201 gtttgttaac gtattctgag ttataatatc ttttttggaa aagtcgttaa ctagtttaat
     1261 gctgttgaca agtttttctc tgggtgaaga tagtccatag acaatgtctc cctcgcgaaa
     1321 gtgtaaggcc acatccagag cgttgccctg aaaggtgatg ttttttgctt taactacatc
     1381 tggagagagt agtacatttt gtaaatcaag atccagccca tcaggcatcc cttcaacacc
     1441 gatgttccac gtggttttct gctccgcatg aatggttacc gatgtcacac tctccggaag
     1501 tatctccggc agtataatat tgctacatcc agtcagcgat agcgtctgca gtgacggtga
     1561 tatcaggttg tcaattctgg cctgattctc cgggttatag ctattgatgc tgagagatga
     1621 taacccgttt ggaacatttt ttatgctgtc tgtggcgcta ccttttatct caaggtagcg
     1681 gacgctctct ggcactccag atatatggca gcgacaaacc gtcaggcatt cgatgccttc
     1741 aggtatgatg tgtataaatg gtgaatcagt taattcaata tgtagatttt ttactgtcga
     1801 tggtatgtct gtgatttgta atttttcaca gttaatgacg gagaggtttt gtgtattaac
     1861 cggcaatcct gtaaggattg tcaggttgtt gcagttttcg attgtaactg atgttatttc
     1921 gtttggtaac tgtggcagat actcaatatc acaatctttg atatataaac gaccactggc
     1981 ccgcgcttct tctatctgaa ttggtgtgat ttcgcttttg atgcctgctg atgtttcagc
     2041 ggaataggag
//
LOCUS       DAZKGS010000349         2050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKGS010000349 DAZKGS010000000
VERSION     DAZKGS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(179..358)
                     /locus_tag="ACHOQY_005069"
     CDS             complement(179..358)
                     /locus_tag="ACHOQY_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9680968.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKEPE"
     gene            450..764
                     /locus_tag="ACHOQY_005070"
     CDS             450..764
                     /locus_tag="ACHOQY_005070"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9680969.1"
                     /translation="MFNTLLAQGAETTKPGSALTLTGLLTTNDSESIEVAMRNHTTHP
                     QGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEARQQSPAGCVMVFAARIRQEVCH
                     VQ"
     gene            754..1218
                     /locus_tag="ACHOQY_005071"
     CDS             754..1218
                     /locus_tag="ACHOQY_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680970.1"
                     /translation="MCNNIRPDAAAEAIITLMHALIDISVIADMAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1211..1444
                     /locus_tag="ACHOQY_005072"
     CDS             1211..1444
                     /locus_tag="ACHOQY_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680971.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDLLHDRYKEQHTGE"
     gene            1450..1749
                     /locus_tag="ACHOQY_005073"
     CDS             1450..1749
                     /locus_tag="ACHOQY_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9680972.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            1746..>2050
                     /locus_tag="ACHOQY_005074"
     CDS             1746..>2050
                     /locus_tag="ACHOQY_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9680973.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANY"
BASE COUNT          555 a          486 c          504 g          505 t
ORIGIN      
        1 ctttaaggaa ttgctcaagg gcaaaagcac atggcgcgaa tctttctgat tcatgctcta
       61 tctttctgcg ccgtcttttc cgtgccggtg ataatgtttt ggtcaattct ttatcggtca
      121 ttgtgttgtc ctgcatagca atgcgccgta ataccttaca ccacggcgct gatggtgatt
      181 actctggttc tttggccttg cgccgctggc gatattcaac ttctcgtttt aatgctgcag
      241 taacaaattg ccccgtactt tcgccaggca tttttaccgc ctcaacattg ttcataactt
      301 catgcggaac ccttgccgca acggtttgtg attttgcgtt tactgctttt gtcgccatgt
      361 ggtgtacccc tcataaaaaa caaatgcagt atgcaggaaa aaaaataagt gttcaacact
      421 tgacgtgttt aacacctggg tttaaattgg tgttcaacac cttgttggcg caaggtgcag
      481 aaacgacgaa acccggcagt gcgctaacac taaccgggct tctaaccacc aacgatagcg
      541 aaagtatcga ggtagctatg agaaatcata ccacacaccc gcaagggcgg gactcgcaca
      601 acctgaataa atacatctgg cgttttatcg ccctgagcac ggcacaaccg cgcgtgatta
      661 ccattgaggc caccagcgaa caggaagcac gccagcaatc tccggctggc tgcgtgatgg
      721 tattcgctgc ccgtattcgt caggaggtgt gccatgtgca ataacatccg tccggacgca
      781 gccgccgaag ccatcataac gctaatgcac gcgctgattg atatttctgt tatcgcagac
      841 atggcgcata agcacgccac cagtgaaaca gaatatgccg gggctttcgt tcctcattcg
      901 ctggcggtta tgcaacttag tgcggatatg gcgctgaatg aggccaaagc catcctgatt
      961 gctgattgtg aaaatggggg ggttatgcgt gatgatcgtt ttaattccct gaaacaggaa
     1021 ttttccggcg ttcctgatga tgcggctgat gcgctttcgt caatgccaga acttattaga
     1081 gcggcttttt tcttactttc cacgagagaa tataaatcaa cggggcttga tgtactgaat
     1141 atcgccgccg attatgcgga atatgtggca gaggcgcgtt atagaagaaa atttcctgag
     1201 gatgtaagcc atgcgtgata tttaccacca aacaatagac cgcgcatttc ttgcactttc
     1261 tcacagtgaa aacatgctgg aaatattgcg catatggctt gaaacacttg gcgacaatga
     1321 acgcgacaaa caaaaatcaa gaattgccac ggcattaata acgcttcttg atcctgtaat
     1381 aatggaactg caagaaatag atctattgca cgacagatat aaagaacagc acaccggaga
     1441 ataaaaataa tgaaacttaa atattctggc ttaactgcca gtggcaacac tcaccctaaa
     1501 tttacgcgcg gtgatattta ccgcgaccag tacggcggca cggtaatgat taagggcgtg
     1561 gaagaacggc gcgtaaccta ccgccgtgaa ggttacgaat atgattgcgt gatgcctgtt
     1621 tatcagttcc ggcgtgattt ttctctggta cagaccgcgc cgcataacgt gcccaccagc
     1681 aacgccaggg cacgggcaaa catccagaag ctgaaaacca tgattaacgg attcaggggc
     1741 aagaaatgaa aagcgcaccg aacttaaaaa aacagcctta cgacaagatg accgaagtca
     1801 ttatttttgc gggtagtgat gcctgggcac atgcgaaaca gtggcaggaa caggacgggc
     1861 gactggctgg cgataatgtg cctcccgttg tgctggctga tgatcaactg gatgaactgg
     1921 cagacctgag aatcatcgac gaggggcgct attgtgtccg gctgtacaag gcaggccaca
     1981 tcaggccatc aaatattaat gccattgcgc acaagctggc ggcggcgggt gtaactgatg
     2041 cgaattatta
//
LOCUS       DAZKGS010000350         2045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKGS010000350 DAZKGS010000000
VERSION     DAZKGS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1755
                     /locus_tag="ACHOQY_005075"
     CDS             <1..1755
                     /locus_tag="ACHOQY_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9680974.1"
                     /translation="SIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPP
                     AVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITADTEH
                     RFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPAGVELTPGYFQITAVPKLT
                     IYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNL
                     VGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTSIT
                     ETRNEITQTVSKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGA
                     GIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAASITS
                     SGNPPAFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIV
                     KTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYS
                     TCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSI
                     SDLLVVVMKKATAGISIS"
     gene            1823..>2045
                     /locus_tag="ACHOQY_005076"
     CDS             1823..>2045
                     /locus_tag="ACHOQY_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9680975.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGD"
BASE COUNT          519 a          513 c          592 g          421 t
ORIGIN      
        1 tccatccggg aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa
       61 aaagaagcca tcgtggataa cggggccagc tttgagccgc agtcaggcac cctgaacagc
      121 gttatccctc cggcagtgca gcacctgacg gtggaggtga gcgcggctga cggtcagtat
      181 ctggcacagg cgaaatggga cacgccgcgg gtggtgaagg gcgtgcgctt cagtctgcgc
      241 ctgaccagcg gaagcggtga gaacagccgc ctgctgacca ccgccatcac tgccgatacg
      301 gagcaccgtt tcagtggcct gcctctcggg gaatacaccc tgacggtcag ggcgattaac
      361 agctatggcc agcagggcga acccgccacc accacgttcc ggattaacgc accggcagcg
      421 cctgccggtg ttgaactgac gccggggtat tttcagataa cggcagtccc gaaactgacc
      481 atttatgacc cgacggtgca gtttgagttc tggttttcgg aggcaaaaat tgcagacgcc
      541 gcacaggtgg aaacctctgc ccgttatctg gggaccggca gtcagtggag tgtctccggt
      601 ccgcacatta agcccggaaa ggatttctgg ttttatgtgc gcagcgtcaa cctggtgggg
      661 aaatctgctt ttgtggaagc cagtggccgg gccagcaatg atgcagaagg gtatctgggg
      721 ctgtttcggg aaaaaatagg aaaactgcat ctggctcagg ggctgtggga gctgatagac
      781 aacagccagc ttgcggatga gatggcggag atgaagacca gcatcaccga aacccgcaat
      841 gaaatcacac agacggtcag taaaacactg gagaaccaga gcgccaccat acagcagata
      901 cagcgcgtgc agaaggacac aaatgatgac ctggctgcgc tgtacatgct gaaggttcaa
      961 aaaacgaaag acggcattcc ctatgtggcc gggattggtg cagggattga ggatactgat
     1021 ggccagccac tgagcaacat actgctgctg gctgaccgta tcgcgatgat aaatccggag
     1081 agcggcaaca gcacgccgtt atttgtggcg caggggaatc agttgttcat gaacgatgtg
     1141 ttcctgaagc ggctgtttgc ggcgagtatc acgtcatccg gcaatcctcc ggcattttcc
     1201 ctgacgccgg acgggcgact gacggcgaaa aatgcggata tcagtggcag tgtgaatgcg
     1261 aactcaggga cgctcaacaa cgtcacgatt aatgagaact gtcagattaa ggggaaactg
     1321 tcagccaacc agattgaagg cgatattgtc aaaacggtca gcaagtcttt cccccgcacg
     1381 agcacttatg ccagtggcac catcacggta agaatcagtg atgatcagaa gtttgaccgg
     1441 caggtcatga taccgccagt gttattccgc ggtggtaagc atgagaattt caacagtaat
     1501 aaccaacagt catactggta ttcaacctgc cggttaagag tgacccgcaa tggtcaggag
     1561 atttttaatc agtccacgac ggatgctcag ggcgtatttt cctcagttat agatatgcct
     1621 gccggacagg ggacgctgac actgacattc accgtatctt catcaggagc gaataactgg
     1681 acaccaacaa ccagtatcag cgatctgttg gttgttgtga tgaagaaagc caccgcaggc
     1741 atcagtatca gctgaatttt ataacccata tacgggcgcc agaaatggcg ccttttttat
     1801 tgcagaaaag cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat
     1861 ttgtctggcc gtatccggtg cgcctgcatg ggcatctgaa catcagtcca cgctgagcgc
     1921 ggggtatctt catgtctcga cgaacgttcc tggcagcgat gaactgaacg ggattaacgt
     1981 gaaataccgt tatgagttta cggacacact ggggatggtg acgtcgttca gctatgcagg
     2041 agaca
//
LOCUS       DAZKGS010000351         2037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKGS010000351 DAZKGS010000000
VERSION     DAZKGS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1557
                     /locus_tag="ACHOQY_005077"
     CDS             <1..1557
                     /locus_tag="ACHOQY_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9680976.1"
                     /translation="PDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFE
                     DEGRNQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYFLFTDKDGE
                     KYGRANKLSGLDKALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDSVQPEPVKVTA
                     DEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQESE
                     QNDQKTEQNSPNAQQNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFDEE
                     NQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTSRA
                     GIHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTIKW
                     WLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRSYE
                     RQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSAIW
                     QKLLPSQADF"
     gene            1625..1867
                     /locus_tag="ACHOQY_005078"
     CDS             1625..1867
                     /locus_tag="ACHOQY_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9680977.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1845..>2037
                     /locus_tag="ACHOQY_005079"
     CDS             1845..>2037
                     /locus_tag="ACHOQY_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9680978.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMT"
BASE COUNT          643 a          482 c          508 g          404 t
ORIGIN      
        1 cctgatgcgc ttcgctacag ccgccagact atttttggcc ttgtccgtga acgtccgatc
       61 gacattcaca aagaccctgt ggcactgaac aaatacatta ctgaatacct gactacaaag
      121 ggcgtgtttg aagatgaagg aagaaatcag agcgcaactg atactctctc gtcgccagta
      181 ccagaaactg atgcagtgga aacggcaatt ccggacaacg aaaaaaccga atgcaaagtg
      241 gaagtcgaac catctgtaga gcgtgagggg ccgttctact tcctcttcac cgacaaggat
      301 ggcgaaaaat acggtcgcgc aaacaaactt tctggtctgg acaaggcgct ggctgccggg
      361 gctactgaaa tcacgaaaga agaatatctt gcccgcaaaa acggaacata cacggactta
      421 ccgcaaaatg tggataccgc tgaagattcc gtacaaccgg agccggtaaa agttaccgct
      481 gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgacgc cgataaattg
      541 cttgctgcat cacgtggtga atttgttgaa gggattagcg atccgaatga tccgaaatgg
      601 gtgaagggga ttgaaacctg cgattctgtg aaccagaacc agcaagaatc ggaacagaac
      661 gaccagaaaa cggaacaaaa cagcccaaat gcgcaacaaa acgagccaga aacgaaacag
      721 gagccggaaa aagcctgcac cgcctgcggt cagaccggcg gcggtagctg ccctgattgt
      781 ggtgcggtga taagtgacgc aacataccag gaaatattcg atgaagagaa tcagcctgaa
      841 gttcaggaaa atgatccgga ggaaatggaa ggcactgcgc atcagcacaa ggagaacact
      901 ggcggcaatc agcatcatgc cagcgatagt gaaactggcg aggcgtcaga tcccttaatt
      961 aaggcgaacg gtcatcataa tctcacatcc accagcagag cggggattca tctgatgatc
     1021 gaccttgaaa ccatgggaaa aaatcctgat gccccgatta tctcaatagg tgcaatattt
     1081 ttcgatccgc aaaccggaga gatggggcca gaattcagca aaactatcga tctggaaact
     1141 gctggcggag tcattgatcg ggacaccatt aagtggtggc tgaaacagtc acgcgaagcg
     1201 caatccgcca ttcttaccga tgaaatcccg ttagatgatg cactgctgca attacgggaa
     1261 tttatcgacg aaaactccgg tgaatttttt gttcaggtct ggggtaacgg tgcaactttc
     1321 gacaacgtga ttttacgccg ttcatatgaa cggcagggga tcccctgccc gtggcgttac
     1381 accaatgatc gcgatgtaag aacgatggtt gctctgggac tggtgatgga tttcgacgca
     1441 agaacgacta ttccattcga aggtgaacgc cataacgctc tgaacgatgc acgttaccag
     1501 gcgaaatacg tttcagccat ctggcaaaaa ctgctcccga gtcaggctga tttttgatgt
     1561 tcaacccata tcgccgccca ccagctatag tggcggcggt catgctgtaa aggcacgtga
     1621 ccacatgtac gaattaactc tatctccagc agagattaaa gagatcacga aatacgagcg
     1681 atacacaaaa cagcaacacc agttaagact gcacggcatc ccatttgtaa tcggccctaa
     1741 aaacgaaccc atcgttctcc gcagggatat tccacacggt ctgacaacga tgccaaaaac
     1801 atctgaactg gtttctgctg aacccgattt tgaggcgctg aacaatggga agaccaagaa
     1861 aaaataaaaa agataatgta ctgccaccgc gggttagatc gaatggttac agttacgtgt
     1921 ggaaacccga aggaagtaca agaagtatag ggctaggaag agtgcggaaa accagcgtag
     1981 ctaaagtctg gcaaaattat gaactggaaa aagcaaaact ccacaacata atgaccg
//
LOCUS       DAZKGS010000352         2013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKGS010000352 DAZKGS010000000
VERSION     DAZKGS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1944
                     /locus_tag="ACHOQY_005080"
     CDS             <1..1944
                     /locus_tag="ACHOQY_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9680979.1"
                     /translation="GERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWL
                     TYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQ
                     HLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATD
                     PAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDE
                     RTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSR
                     KPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQ
                     QHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQ
                     LLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHA
                     GIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVG
                     IGAGVDASVMTLACRTWQECWVN"
BASE COUNT          488 a          498 c          642 g          385 t
ORIGIN      
        1 ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca gcatgagaca
       61 acacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag cctgccgccg
      121 gtggagtggc tgacctacgg cagcggttat ctggcgggca tgaagcttgg cgacacgccg
      181 ctgctggagt acacgcgcga ccgcatgcac cgggagacgg tgcgcagctt tggcagcatg
      241 gcaggcagta atgccgcata taaactgacc agcacataca cccccgcagg ccagttacag
      301 agccagcacc tgaacagcct ggtgtatgac cgtgactacg ggtggaatga caacggcgac
      361 ctggtgcgca tcagcggccc gcgacagacg cgggaatacg gctacagcgc cacgggcagg
      421 ctggagagcg tgcgtaccct cgcaccggac ctggacatcc gcatcccgta tgccaccgac
      481 ccggcgggaa accgcctgcc ggacccggag ctgcacccgg acagtaccct cacggcgtgg
      541 ccggataacc gcatcgcaga ggatgcgcac tatgtttacc actatgatga atacggcaga
      601 ctgacggaga agacggacct tatcccggcg ggtgttatcc ggacggacga cgagcggacc
      661 caccactacc actacgacag ccagcaccgt ctggtgttct acacgcggat acagcatggc
      721 gagccactgg tcgagagccg ctacctctac gacccgctgg gacggcgaat ggcgaaacgg
      781 gtatggcggc gggagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa accggagatg
      841 acgtggtacg ggtgggacgg cgaccggctg acgacggtac agaccgacac cacgcgtatc
      901 cagacggtat atcagccggg gagcttcgcg ccgctcatcc gcatcgaaac agacaacggc
      961 gagcgggaga aagcgcagcg ccgcagcctg gcggagaagc tgcagcagga agggagcgag
     1021 gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc tgctggacag gctggaggaa
     1081 gaaatccggg cagaccgcgt gagcagtgaa agccgggcgt ggcttgcgca gtgcgggctg
     1141 acggtggagc aactggccag acaggtggag ccggaataca caccggcgcg aaaagttcat
     1201 ttttaccact gcgaccaccg gggcctgccg ctggcgctca tcagcgaaga cggcaatacg
     1261 gcgtggcgcg gggagtatga tgaatggggc aaccagctta atgaagagaa cccgtattac
     1321 ctgcaccagc cataccgtct gccggggcag cagcatgatg aggaatcagg gctgtactat
     1381 aaccggaacc ggtactatga cccgctacag gggaggtata ttacacaaga ccccattggg
     1441 ctggcggggg gatggaacct gtataattac ccactgaatc cgataataag gatggatcct
     1501 ttgggtttgt ataatttata tcaattatta tatgatgttt ggcatgatga ttcatatgga
     1561 acatcatcaa ttgatattac tggcagtgga gatctaatat cattaggtgg tcatgcagga
     1621 cttggcgttg cgtttgctaa aaagaaaggt gaaatgttat ctgatatttg tatttatgct
     1681 acagcatgcg gacatgcagg aattggtggt gggataaatg cggctatcac atattctgag
     1741 accaagtctt tacctgcatc gggagtcagc aattcagtag gtgtaacggt tggcggcgga
     1801 gttggggggc attttgcgta tacttatgta gtggatgttg ataatccaga atcatcgaca
     1861 gaatctgttg gtatcggtgc aggtgttgac gcttcagtta tgactctggc ttgtagaacg
     1921 tggcaagaat gctgggtcaa ttaacaggag gaaatatgaa aaaatatata tttttagttt
     1981 tatttttatt gtgtggttgt tctccagtgg aca
//
LOCUS       DAZKGS010000353         2000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKGS010000353 DAZKGS010000000
VERSION     DAZKGS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..752)
                     /locus_tag="ACHOQY_005081"
     CDS             complement(<1..752)
                     /locus_tag="ACHOQY_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9680980.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYL"
     gene            760..1261
                     /gene="tnpA"
                     /locus_tag="ACHOQY_005082"
                     /pseudo
     CDS             760..1261
                     /gene="tnpA"
                     /locus_tag="ACHOQY_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1279..1947)
                     /locus_tag="ACHOQY_005083"
     CDS             complement(1279..1947)
                     /locus_tag="ACHOQY_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9680981.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT          500 a          474 c          461 g          565 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata caggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
      121 agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgagccgga
      241 gtggatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaacacc cgtgacctga cggctattcc ggtagcgcat ccagcccagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt
      481 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta
      541 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccggc ctcatgattc tcattctgac gtgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgacc acctggtctt
      721 aactggaatt taaatgcctg taatcgtttc acaggtcaaa tgatactttg gtctgtgaat
      781 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      841 tttgtcacca gataccgacg ccagattttt tgacgcgaca gaaaaactac gcacttactt
      901 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      961 cgtccatttg ttaattaact aacctcccaa actggcgata tccagtctgg taaacagcct
     1021 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
     1081 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
     1141 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcac
     1201 cttatatccc cgccctgaag gatggggttt tacggcacac cggataaaaa gcgccatcta
     1261 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
     1321 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
     1381 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
     1441 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
     1501 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
     1561 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccagtgg
     1621 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
     1681 aactttctgc cctacctggg tagaaggttt tggcaccgga taataagtga ttgggttcca
     1741 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     1801 acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     1861 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     1921 aactttcgca aatgatgtgg tacgcatgaa tagtttcctg agatgattat attacttttt
     1981 aagttatagc aaaaagcgcc
//
LOCUS       DAZKGS010000354         1965 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKGS010000354 DAZKGS010000000
VERSION     DAZKGS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1802)
                     /gene="nleL"
                     /locus_tag="ACHOQY_005084"
     CDS             complement(<1..1802)
                     /gene="nleL"
                     /locus_tag="ACHOQY_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP9680982.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     SFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYH"
     gene            complement(1822..1911)
                     /locus_tag="ACHOQY_005085"
     CDS             complement(1822..1911)
                     /locus_tag="ACHOQY_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680983.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
BASE COUNT          665 a          403 c          291 g          606 t
ORIGIN      
        1 ttatgataag gaatactgaa aattggaaat gattcagaaa aaagattgct caatgaatac
       61 ccaaatgtat tttgaacttc tccatcctta tataaaaaaa aggacatcca gttagttgat
      121 gtattaggag ataacatatc aaccatatga ctcaaagaac acatcattgc cataccatca
      181 tcctgtgatg ataacaaaac ccagttataa gccaaatcgg aatcatcatc ccagtcaggc
      241 ttcccagagc catcgcacaa tccaaaatca ctctgctgtg tgacaggata aactttttca
      301 ttttttaaat atacttcata gagttcccta aatcgttcct gcattccatc acgccgtgaa
      361 ccattcatta caatttgtat aaaggaacta ttataagata ccatcgcacc tggatgtcgc
      421 tcaaaataca tccctgcctg caataaacta ccattctcca tatacatcat tattgaatta
      481 ttaaaccaag acattatagt atcaaaaaag tcatcacata taggctcgag ccattgctgt
      541 atatttgcgt tgttcaaata aggatgaata ctaaatgaat cagccaaaga catcttaaca
      601 tagggccgga acgccgctaa ttcgtcaaga gatatttttt tgataagttc ttcagcggcc
      661 cttactttat catggttata cttatcatca gatgtattca tagtcaataa aattgattta
      721 tttgattttg tatagttatc caacacagat aatatatttt cctgtatgtc agatagatct
      781 atgtaacacc ctcctagatt aatggtatta cttttttcac taaagagctc ctttacatca
      841 cctaagatag caccacttaa aaccattcca ggaattataa tagctcctgt taaatcagcc
      901 tcgttcaaaa ttggaggata ttgtatatat gaagatcctt taaatgatgc atttttaaga
      961 ttggaattgc tgaaattaga gttttcaaac aaagccctaa taatacaatt agataaatta
     1021 gcataacaaa aattacattc attcaaaatc gagtttttaa aagaacagtt ttccaataca
     1081 gcctgagaca aatcactcat cctgagatct gtatcatcaa gcactgctcc agaaaaattt
     1141 actgatgata aattcagccc tacaagactt agacctgaca agtcacatcc agagtaattc
     1201 aattcttcag cagactcttc acccgttcgg ttagccgaca accaaattaa gtcagcagta
     1261 agctcagctt tgctcaggca ggcacggcct tgactgacat catatgcaag gaattgacat
     1321 tgcgattcat ttaatgaatt acagccattt aaatatacgg aatttctgag tgcctcagga
     1381 aaggaacctt ctatatgttt caaagaacta caatggcata aagataaatt aatgacattt
     1441 tcaggtatgc tgcaatttat aacctctaat gatgagcatc caaccatact taatgaagat
     1501 aagttggggg gtaagcagtt tattgattta agctctgtac atccattcaa aaccagttct
     1561 ttcagagaac ctggaagcaa gtctggtaat gttgttattg gctcactgat tgataaagtc
     1621 tctccattag tacttataac atcaagtatt tttgatgcaa cctcatgacg attttcgcta
     1681 aattctccct cagcgcacca cttttcgagg gcaacttcaa catcctcgtt agatggtgaa
     1741 tctacaggac tttcaaaaga tattactcca gaatttacag agatatttgt agtgggcagc
     1801 atttatctca ctccttttta cttagtccac taaagtaaaa ttagaaaaca caactgccgg
     1861 gacaactttc tgtccctgta cccacaaata agatgtcaga tttatcggca ttaatttaac
     1921 tccctattga attatctcct cttttttatc taacaaatat tcccc
//
LOCUS       DAZKGS010000355         1943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKGS010000355 DAZKGS010000000
VERSION     DAZKGS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            226..1458
                     /locus_tag="ACHOQY_005086"
     CDS             226..1458
                     /locus_tag="ACHOQY_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9680984.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMMNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            1461..>1943
                     /locus_tag="ACHOQY_005087"
     CDS             1461..>1943
                     /locus_tag="ACHOQY_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9680985.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     A"
BASE COUNT          596 a          365 c          383 g          599 t
ORIGIN      
        1 tccatatagt cgtacctcat gtactcctaa acactctgcg gcaataaaat atttttttcc
       61 tcaacaaatt actgcaaatg agatatgcat cactatttgc agttattcat cattaaaatc
      121 ttatttgaca gttgattggc cttgatttgt gttgataatg gcgtgaatgc ttatgtaggc
      181 gtacagcatg tactacttaa agatattatt cacgaggtaa atataatgag actttcaaat
      241 atcgtcacag ttataattct tgcaatttcc agtacgctaa caccccaggc gatggcagaa
      301 aaattgattc ctgaaactga tgctgagtta ttaatctggt ctgatgccac aagcgcggaa
      361 tatatgaaat atgcagcaac ggaattcaat aaagattttg gctacaaggt caagtttaca
      421 tttcgcaata tagcgccaat ggatgcagca tcaagaatta tgcaggatgg gggtacgact
      481 cgtgtagctg atgtagctga aattgaacat gataccctgg ggcggttagt cgttgctggc
      541 ggggttatgg aaaacatggt ctcagctgag cggattaaaa aaacatttat tccaggcgca
      601 gtatcggcag ctacatataa taacatcagc tatggttttc ctgtaagttt cgcaacgctg
      661 gcgcttttct ataataagga tttgttaaac accgcaccaa aaacattcga agaaatcaat
      721 actttcagtg aaaagtttaa taattcatcc gagcataaat atgctctgct atgggatgta
      781 caaaattatt atgtttcacg tatgtttatt accttgtatg gtgccaacga attcggtaaa
      841 accggtaacg atcctaaagc tctaggcatc gcttcatctg aagcgaagaa agggctagag
      901 acaatgaaac gcttaaagaa agcgaatcct tctaatcctc ttgatatggg taatccacaa
      961 gttctaagag gtctgtttaa tgaaggaaaa gttgctgctg taatcgacgg accttggtcc
     1021 atacaaggtt acattgacag cggaatcaat tttggcgtga cacgcatccc aacattagat
     1081 ggtcatcagc ctcgcacatt ttcaacagta cgactggccg ttgtaagctc atttaccgaa
     1141 tatcctcatg ccgctgaatt attcgctgac tacctgacaa cggataagat gttgatgaag
     1201 cgttatgaaa tgatgaatct catcccacca atcgattcac ttatgaacaa aattagccag
     1261 acaggtagtg aagctataaa agcaattatt gctcaagcta attattctga cgcaatgcca
     1321 tcaataccgg aaatgtctta tctatggtct ccaatgacta atgctatttt ggctacctgg
     1381 gttgagaata aaacaccgga tgaagtttta aatcatgcac aaacaattat tgaagaacaa
     1441 ctttcgcttc aggagtaatt gtgaatacac aacaactgaa aatgaagagt gcacctgtgc
     1501 tacctatttc ctgtttgatt atgggcggga ctcagttatc acgtcattat tatgtgaaag
     1561 gcgggatatt ctttgcaata caagtatgct ttttgcttta tctttccgat attgtgcata
     1621 cattaatagg tttgttcaca ttaggtgatg tcgcgcaaat tcgaaaaggg ttgactgtta
     1681 ttcagggtga caattctata tttatgcttg tagagggcgt gatagcgacc attattgttg
     1741 gcttattcgc cactatttac atccttaata ttaaagatgc gcggaactca tcttattgcc
     1801 atctgacttt taaacaacag ttatataagc tctatgagga taaatttgca tttatagtcc
     1861 taacgcccgc atttttagcg agtatagcat tcattgtttt acctattgta attacagttc
     1921 ttgtatcgtt tacaaactat gct
//
LOCUS       DAZKGS010000356         1941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKGS010000356 DAZKGS010000000
VERSION     DAZKGS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..202
                     /locus_tag="ACHOQY_005088"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHOQY_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHOQY_005089"
     CDS             331..576
                     /locus_tag="ACHOQY_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680986.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHOQY_005090"
     CDS             548..958
                     /locus_tag="ACHOQY_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9680987.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..1131
                     /locus_tag="ACHOQY_005091"
     CDS             955..1131
                     /locus_tag="ACHOQY_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9680988.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            1134..1535
                     /locus_tag="ACHOQY_005092"
     CDS             1134..1535
                     /locus_tag="ACHOQY_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9680989.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            1495..1704
                     /locus_tag="ACHOQY_005093"
     CDS             1495..1704
                     /locus_tag="ACHOQY_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9680990.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEYCCAELMSDPNSSMYEEEDDE"
     gene            1697..>1941
                     /locus_tag="ACHOQY_005094"
     CDS             1697..>1941
                     /locus_tag="ACHOQY_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9680991.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQ"
BASE COUNT          612 a          386 c          490 g          453 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatgcttt
     1141 taattcaacc tggatttgga cttagcatca aaaaaggcca catgtttggc gagaaagagt
     1201 cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg ctaaacaggg
     1261 aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct gactggtttg
     1321 tgaaaaacca ccacgcagtt cgtcaggcaa agagaaaggc caatatgaca tacatgaagg
     1381 cgtatcaaaa agcatggaaa gaacaccgcg atcgatacca acaagacatg gaaaagcttg
     1441 aatcagaaaa catggaatta agacgaaagc tcggtgaagc aaaacgagac attgatgctt
     1501 acaagcgact ttttaatggt gaaagccatg cttagcccat cccaatccct tcaataccag
     1561 aaagaaagcg tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg
     1621 gaagttcacg tgtgtgagta ctgctgcgca gaactgatga gcgatccgaa tagctcaatg
     1681 tacgaggaag aagacgatga atgagttaat aaatggcaat gccatcaaaa tgacaagcat
     1741 tgaaatagct gagttggtgg gtaagcgtca tgacaatgtg aaacgtacca tcgaaacgct
     1801 ggctaaaaat ggtgttatcc ggcttcctca aattgaggtt tccgaaagaa tcaataactt
     1861 agggttcaat gttcagtacg agcattacgt cttcgagggc gaacaaggta agcgagacag
     1921 tattgtcgtt gttgcccagt t
//
LOCUS       DAZKGS010000357         1927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKGS010000357 DAZKGS010000000
VERSION     DAZKGS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..122)
                     /locus_tag="ACHOQY_005095"
     CDS             complement(36..122)
                     /locus_tag="ACHOQY_005095"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9680992.1"
                     /translation="MSVLADCVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(667..743)
                     /locus_tag="ACHOQY_005096"
     tRNA            complement(667..743)
                     /locus_tag="ACHOQY_005096"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Arg,seq:tct)
     gene            complement(841..916)
                     /locus_tag="ACHOQY_005097"
     tRNA            complement(841..916)
                     /locus_tag="ACHOQY_005097"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Ile,seq:cat)
     gene            complement(966..>1927)
                     /locus_tag="ACHOQY_005098"
     CDS             complement(966..>1927)
                     /locus_tag="ACHOQY_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9680993.1"
                     /translation="LIVTDPPYFKVKPEGWDNQWEGDDDYLKWLDQCLAQFWRVLKPA
                     GSLYLFFGHRLASDTEIMMRERFNVLNHIIWAKPSGRWNGCNKESLRAYFPATERILF
                     AEHYQGPYRPKDDGYVAQGRELKQHVMAPLISYFRDARKSLGITSKQIAEATGKKNMA
                     SHWFGTSQWQLPNEGNYNKLQALFARVAAEKHQRGELEKPHHQLVSTYSGLNRQYTEL
                     LSEYKNLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQIINASSRPGDLV
                     ADFFMGSGSTVKAAMALGRCAIGVELETGRFEQTVREVQDLIV"
BASE COUNT          510 a          530 c          397 g          490 t
ORIGIN      
        1 gtatttaaaa atttctcccg ctaactgata cccggctaac agtctggcgt tttctttttc
       61 agcaacggga aagcaacaac caccacaccc gccaccagta cacagtcagc cagcactgac
      121 attatccggc tgctgcaatg ccattcacaa aaacagtaag caatcacttt ttaccgtaac
      181 aggtgataat ccagatatgt atctacccca gatgagtaat ccgaagttca tccataccac
      241 aggtcctggc tattctgttg tactcctgaa caagagcaaa taattctgaa ttagcaacca
      301 tgaactcatc gcaaaccctc tgtatagcat cactattcag aagaataacg tctcttcccg
      361 aaagacgatc aggagtacag aacaaaactg tcaaacggct gaaggccttt gctcgtgctg
      421 cattgactat atcaatacgc tgcctaagga tgaaacaccc cgacgcctca tcaatattca
      481 ctctacccac accatatgaa tgataaatat ttaatactga aaaaaccatt agaccgtata
      541 acaaacactc aatcaatact taacagaact tttatttttg acaaacatat aatattttca
      601 acaatatcct gagccaggta tattccagta taaggctctg ccggaagaaa tctggaagaa
      661 tgaatatggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
      721 ctgtccggct gagctaacaa cgcataatgc agataatgga ttgccatcgg ggacccgggc
      781 cccacacagc cagtttcgaa agctgacgct ctctgccgat gagctaatgg cggtatgtga
      841 tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct cgctctcacc
      901 actgagctaa agggccggta gcagaataat aatggtgcgt aattaattct gcaatcccat
      961 ccgtttcaaa cgattaaatc ctgaacttcc ctgactgtct gttcaaaacg tcctgtctcc
     1021 agctcaacac caatcgcaca acgccccagt gccatcgccg cttttaccgt tgaacctgaa
     1081 cccataaaaa aatctgcaac caggtctccc ggacgactgc tcgcgttgat tatctgctgc
     1141 agcatttctg ccggtttttc gcacggatgt ttccctggat agtactgcac cggtttatgc
     1201 gtccagacat cggtgtacgg aacctgcgcc gtcacaccga aataccgccg caaattttta
     1261 tattcactca gcagttccgt atactgccgg ttcagcccac tgtatgtgct gaccagctgg
     1321 tggtgtggct tttccagttc cccgcgctga tgtttttctg ccgcaacacg cgcaaacaac
     1381 gcctgcaatt tgttgtaatt accctcgttc ggtaactgcc actgactggt accaaaccag
     1441 tgcgaagcca tgtttttctt tccggtggct tccgctatct gttttgacgt tattcccagt
     1501 gatttacgcg catcacgaaa gtaagaaatc agcggggcca tgacgtgctg ttttagctcg
     1561 cgcccctgtg ccacatagcc atcatctttc gggcgatacg gtccctgata atgttctgca
     1621 aacagaatgc gctctgttgc cggaaaatac gcccgcagac tttccttatt gcacccgttc
     1681 cagcgtccgg acggcttcgc ccagataata tggttcagca cattaaagcg ctcacgcatc
     1741 atgatttcgg tgtcagatgc caggcgatga ccaaagaaca ggtaaagact tccggcaggc
     1801 ttcagtaccc gccagaactg cgccagacac tggtccagcc atttcaggta atcatcgtcg
     1861 ccctcccact ggttatccca gccctcgggc ttcactttaa agtatggcgg gtctgtgact
     1921 atcagat
//
LOCUS       DAZKGS010000358         1922 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKGS010000358 DAZKGS010000000
VERSION     DAZKGS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..182
                     /locus_tag="ACHOQY_005099"
     CDS             <1..182
                     /locus_tag="ACHOQY_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9680994.1"
                     /translation="GWFPDTDGALITGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDV
                     KGLDEVGSDTGRTGE"
     gene            184..453
                     /locus_tag="ACHOQY_005100"
     CDS             184..453
                     /locus_tag="ACHOQY_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9680995.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            455..523
                     /locus_tag="ACHOQY_005101"
                     /pseudo
     CDS             455..523
                     /locus_tag="ACHOQY_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            564..1145
                     /locus_tag="ACHOQY_005102"
     CDS             564..1145
                     /locus_tag="ACHOQY_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9680996.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            1213..1848
                     /locus_tag="ACHOQY_005103"
     CDS             1213..1848
                     /locus_tag="ACHOQY_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9680997.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCVFRPDSNHFSCPESFLTCPITLDTPENGVFMRNSRGAEI
                     CSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDSKKEAFVASDA"
BASE COUNT          559 a          329 c          502 g          532 t
ORIGIN      
        1 atggctggtt cccggataca gatggcgcac tcatcaccgg actgaccttt cttgacccca
       61 aagatgccac acaggttcag gggctgtttc ggcatttgca ggtcaggttt ggtgacgggc
      121 cgtggcagga tgttaagggg ctggatgaag tgggcagtga tacaggcaga acaggagaat
      181 gacatgaata tactaaaaaa acttatgcag cgtctgtgtg gttgcggaaa gcatgatggc
      241 cgtgaacacg tgcagtcgct tacagcacaa ctgcgactgg ggccggcaga catcctggag
      301 tccgatgaga atggtattat cccggagcag gacagggtaa tcacgcaggt ggtgatactg
      361 gatgcggata aaaagcagat acagtgtgtg gtaagaccgc tgcaaattct gcgtgctgac
      421 gggacgtggg aaaatattgg cgggatgaag taacccgaca gcttcacaaa accggagtcc
      481 ggctccggtt tttgttgtca tgtcagggag atgtttgtta tgaagcccag aggaaatatt
      541 tatctgtatg aaggaatatg gtaatgcctg gattagtatc atatatatca tcgacttcat
      601 tcgcgaatga gatggcggaa atgcgtcagc aggtaatgga agggcagatt ggaggatttc
      661 tcctgggagg ggagagagtt agagtttctt atatgccaga tacaggccgt tttttagcag
      721 aaagtgaagg gctgggactg gtttatgcag aattattgaa tattggtttt aatgatggag
      781 ttgatgcgct cagaaacagg gtgttaagcg tgcttcctgg aatggtggcg cagcgacaag
      841 agaactcttt gcaggccaaa atatcggaat gtacctttac tgttgatatt gaaaaacttc
      901 actgtcctgg tgaggtgctt caatgtccaa ttacactgga gcagcctgaa aaaggtattt
      961 ttgtgaagaa ttcagatggt tcagatgtat gtactttatt tgatgccgct gcattttctc
     1021 gtttgactgg tgaaggctta ccccacccac tgacccggga accaataacg gcatcaataa
     1081 ttgtaaaaca tgaagaatgc atttatgacg ataccagagg aaacttcgtt ataaagggta
     1141 attgaaatga acattaccct ttatttaatg aaacatcctg caaactgata tgaattactg
     1201 aatgaggttt ttatgcctgt taccacctta agtatcccaa gtatatctca attatctcct
     1261 gcaggagtgc agtctttgca ggatgcagcc agacttgaaa gtggaataag aatatccatt
     1321 ggtagtggcc aatattctgt tcactatgtc caactactgg atggattttc agttgaaccg
     1381 gtgagaggag gcttactgga taggctattg gggcgtgagc atcgaatgga gagaagggct
     1441 gtggctctgg aaaggcaatt aaatggaggt gtcgattttt taagtagtgt taataactat
     1501 tttcagagtg tcatggcaga acacagagaa aataaaacaa gtaataaaat attaatggaa
     1561 aaaataaatt cttgtgtatt tagaccggat tctaatcact tttcttgccc ggagtcattt
     1621 ttgacatgcc cgataacgct ggacacacct gagaatggag tgttcatgag aaactcacga
     1681 ggtgctgaga tatgctctct atatgataag gacgcgttag tgcaacttgt tgaaactggt
     1741 ggagctcatc ctctgagtcg agaacctata acagaatcaa tgatcatgag aaaagacgaa
     1801 tgtcactttg attcaaaaaa agaagccttt gttgcaagtg atgcttaatt ttttctgttg
     1861 gtgtgttttt atattaatag tttattataa tagtgccatg taaggatata ttgcctgaac
     1921 aa
//
LOCUS       DAZKGS010000359         1892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKGS010000359 DAZKGS010000000
VERSION     DAZKGS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            659..949
                     /locus_tag="ACHOQY_005104"
     CDS             659..949
                     /locus_tag="ACHOQY_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9680998.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            973..1224
                     /locus_tag="ACHOQY_005105"
     CDS             973..1224
                     /locus_tag="ACHOQY_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9680999.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1272..>1892)
                     /gene="traT"
                     /locus_tag="ACHOQY_005106"
     CDS             complement(1272..>1892)
                     /gene="traT"
                     /locus_tag="ACHOQY_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFP9681000.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          491 a          386 c          438 g          577 t
ORIGIN      
        1 gaatatgcat tataaaatat cagttaacat taacaggatt ttgtaataag ccacatttat
       61 tctttcggga attaaaaaac ataacgaaat tttgcctcat tctgcatttc attgaaatag
      121 tatgtttctc atcgaaagca agtttgtcaa tataaatttg taacagaaat gactttccgg
      181 ggatgaacaa aactgttcat ggcaatggct ttctctgtcg ccaacatgga tacattgttc
      241 cggataaagc actgatgtca ttgactgacg cagactatca ggccatctga tgctttcttt
      301 tccttcaccg gtattgatga tatgccttat gaagggggac ttattcctgt aacccaatca
      361 caggcactgt gattactggt ttatgattga tgctgatttg agcagatatg cacagcagtt
      421 gtggattcag gggatatttt ctgccagata ttcataccag attgatgacc tctttgctca
      481 gatgctcttc gttctgagcc agccaggatt ttgtggccac atacattgtt cgccgatggg
      541 gaatcgagac aggcgattgg ctgtgatgcg attttgccgc taactgagga tgagtccgcc
      601 cctcccgggg aattcctgta agttaagccg tacctgttga ttagcactaa actgggtaat
      661 gaatttatct gccagtatga atgatgccgg ggacggtttt gaactcctga tgtcgggtaa
      721 tatgcaggat tttaatattt gttgtcgtcg ggataatcat tatcctgaac ataaactgat
      781 acctttatca gagttatttg acgttaatta tggactgaat cttgagctga ataaactgga
      841 gaaagattct tcaggaatta attttgtttc aagaacgtcc aaaaataatg gcgtctccgc
      901 cagggttaaa ttaaaagatg gtattagtcc atgccagcag ctgtgttaac tgtagcagca
      961 ggaggttctg ttatggaatc gtttgtacag gattcaccat tttatagtgg aagagatctt
     1021 tattggttac gtcctaaagt tgaactgact ctggaagaga aattgtacta ctgctcttgt
     1081 ataagaagaa acagacacaa atacagctac gggcgtcagg caaaccgaac gctgaagaat
     1141 ttactggtac cgtcattaga ttctgttccg gcatgggtat atggtgtgac agggaaaata
     1201 atatctgaat tgtctgaacg ttgagactaa gagggccgga ggccaggtga atggccaccg
     1261 ggatatttat atcagagtat attggcgatt gattttgcca gctggtcttc cagatgaggt
     1321 ttagcttctt caaacttcag attaacctgg ttggcactgg atacaacccg ggtctggtat
     1381 ttatgctggt tgccggtttc tgtgcttgtc tgaattttgg agcctgatgt tccctgacgc
     1441 aaagctgcaa cattatccgt tctgacgctg gttctggttc gctcggcaat ctgaacatct
     1501 gtaatcattg tgtaatttat atcttccacc attgcatccg cggccatgcc aatcagaccg
     1561 gtagccagtc caacaccgag agtggtacct gccgacccgg tattgtacgc tgtgattcca
     1621 cctccaagag cagctccgac tgctgcacct tcataaccac gactcagcca tccctgagat
     1681 tcacgcagat ccattttgtc tgctttgaga acgttagcct ggatccagta gtaagcttta
     1741 tccggggaag tcatgatctg ataacctctg gctttgactg catcggcaat ttttccctgt
     1801 agtccactca tgtctttatc cgacgtgttt ttgatctgca ggaaaacggt gcgttcgctc
     1861 gcaggttcaa gccagatggt tgaggtgtac tg
//
LOCUS       DAZKGS010000360         1889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKGS010000360 DAZKGS010000000
VERSION     DAZKGS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..1488)
                     /gene="pykF"
                     /locus_tag="ACHOQY_005107"
     CDS             complement(76..1488)
                     /gene="pykF"
                     /locus_tag="ACHOQY_005107"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9681001.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1496..1690)
                     /locus_tag="ACHOQY_005108"
     CDS             complement(1496..1690)
                     /locus_tag="ACHOQY_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681002.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          476 a          443 c          461 g          509 t
ORIGIN      
        1 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
       61 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      121 cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc tctgcagagc
      181 cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg
      241 cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag
      301 gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgagtagc
      361 aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      421 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      481 gtcggtacgt tcgcaaatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      541 tgcggattca ccagacagca tcactgcatc agtaccgtcg aggatggcgt ttgcaacgtc
      601 accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc
      661 agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      721 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      781 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      841 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
      901 cttacgaata aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
      961 ctgtttgtct ttttcagcca gtgctggcag agcaatggaa acgccaggca ggttcacacc
     1021 tttgttttcg cccaggtcac cgttgttcag cactttacag ataactttgt taccttcaat
     1081 ggcggtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc caactgacag
     1141 gtcagtagtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1201 atcagtggtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag
     1261 tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1321 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1381 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1441 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1501 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1561 cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1621 gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1681 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt
     1741 aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat
     1801 tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcgc tgtttgcgca
     1861 acgtctggtg ccaaagttgt cagtgtgag
//
LOCUS       DAZKGS010000361         1887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKGS010000361 DAZKGS010000000
VERSION     DAZKGS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1481
                     /gene="espN"
                     /locus_tag="ACHOQY_005109"
     CDS             <1..1481
                     /gene="espN"
                     /locus_tag="ACHOQY_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFP9681003.1"
                     /translation="ILEFVHEINNISSARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQ
                     QFIWGFDKVIQEGLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAV
                     PGYNALSGVSSILHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEAL
                     KHIEKNINDNIIYHPSNNANFYSVIESIDGNDFIYNEKQSKILEMKQDRGGNRYSAVD
                     LNSSKYGYYEKVGGGFYRYIESFNPISSETPNKIVYKGESVDLTKEPNSELYSGRYSI
                     NNKQVNVYFFRDADGTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSP
                     VLEDTLPALSSEIVPTPEHSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYK
                     TAGGLPDSNPNVKAPDEYMAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKY
                     ITRGSVTESGWVIDKTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          614 a          291 c          401 g          581 t
ORIGIN      
        1 ctattcttga gtttgtccat gaaattaata atatatcatc tgctcgctgg atgtcaaaat
       61 cagaaaagaa taataaacaa aaagaggcaa tagaaaagtt caaaaaagaa gtatcccata
      121 tgaatggcgg gcagcagttt atttgggggt ttgataaggt tattcaagaa ggcttaagtg
      181 gattgattga gttaagtatc gatattaatg atagtacaaa tcatcgtgat aagtcttctc
      241 tttctcctga tgggagagct gtgttacatt ttttaggtac aatttggaat atggcgatgg
      301 gagctgtacc tggttataat gcattgtctg gtgtaagtag cattttacat agtgctatag
      361 taaaagaatc tagcaatatc tgtgattata ttcagggggc tgtacgtatt ggaatggact
      421 ttgttccagg cactcgctct gacttacata gccgttcgct gcagataaaa tatgaggctt
      481 tgaagcatat agaaaaaaac attaatgata atattattta tcatccgagt aataatgcta
      541 atttctattc tgtaattgag tcaattgatg gtaatgactt tatatataac gaaaaacaat
      601 ctaaaatatt agaaatgaaa caggatcgtg gggggaatag atatagtgca gtagatctta
      661 actcttctaa gtatgggtat tatgagaaag ttggcggtgg tttttataga tatatagaat
      721 cctttaaccc catatcttca gagacaccaa ataaaatagt ctacaagggg gaatcagtag
      781 atttaactaa ggagccaaat tcggaattat attcaggtag gtattctata aataacaaac
      841 aggttaatgt ttatttcttt cgtgacgctg atggtacatt ttataaatca gaaggtcttc
      901 atggtggggg agttattaga tacatagata aaccgtattc tcagttaaga gaaggagata
      961 ttgggtatga tgaggatttg ttggatatat acgatgattc tccggtgctt gaagacacgt
     1021 tgcctgcttt atcttctgaa atagtaccaa ctccagaaca tagtattaaa caaatttatt
     1081 cgaaaattaa ggaggggcac atagaactgt ccgattcaga catcatattg tgtcgcggca
     1141 caaccggtat tcaagctgaa aatatcgttg aatataaaac tgctggaggg cttcctgatt
     1201 caaatccaaa tgtaaaagca ccagatgaat atatggcaca acagcaggta cgtattggaa
     1261 gaatattgcc tgaatacaca tcggatctta gcgttgctga tcggtttagt cgtgagcatt
     1321 atctaatagt tgttaaagta aaggcaaaat atatcacacg aggaagtgtt acagagagtg
     1381 gttgggttat agataagacc gcacctgttg aaccacttgc gataattgat agaacttttg
     1441 gtatgaagga aaatatctca atggtaaatg catcgaaata gtttttttac aatctatgtc
     1501 ctgcctcctc tggtaaaaac gatgctacat ctttgagatg ttgcacggca gtacggtgtt
     1561 gaccgatata tagtgaagta cacatcggtc aacgaattac cattgtcagc aatatcatcc
     1621 tgataaactc agtactcgtg agccgcttga tgacgggcgg aatagcccaa aaggtaaacg
     1681 atccgcatgg cagtccgtcg gaaagctgct gcttcatacg accgcttaaa ccgtcagtta
     1741 gtgtcagtat cgctgaagat cagcttcttt tgctgattta ctctgttttt acctgccctg
     1801 atgaaatact ctttaatcat aacattgatt atatcgaaaa tatatttttt gctatcatta
     1861 agaatattta tatgtggaac cggagta
//
LOCUS       DAZKGS010000362         1881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKGS010000362 DAZKGS010000000
VERSION     DAZKGS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..472)
                     /locus_tag="ACHOQY_005110"
     CDS             complement(56..472)
                     /locus_tag="ACHOQY_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFP9681004.1"
                     /translation="MNKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKAC
                     IAKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWS
                     EQFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            complement(772..1380)
                     /locus_tag="ACHOQY_005111"
     CDS             complement(772..1380)
                     /locus_tag="ACHOQY_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFP9681005.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1806..1874)
                     /locus_tag="ACHOQY_005112"
                     /pseudo
     CDS             complement(1806..1874)
                     /locus_tag="ACHOQY_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          512 a          419 c          395 g          555 t
ORIGIN      
        1 ttttttaaca aaaattgacc tgagaattcc atatcttttg tcaactctct gccgattacc
       61 agtgtgcttt tacccatttc aaaactccag aattttggtc acctacagca tcaaacaatt
      121 gttcggccat agctttactt atgttattgt cgtatctaaa ttgctcgctc cagtctttaa
      181 cgaccgacca gttaatctcc aacgaaacat cattttttgc gtcagcaatc aaaatttgat
      241 gtaagttggc aattttgaga agttgcgaca aatcatgagt atatgaatca tttacgactt
      301 ttttgtttgg aaactcatgt tgtaaaaatg acttggcaat acaggccttc aatgcacatt
      361 caactgcata cccacaaaga taatatgcac catgaaaaaa gccatgatca agcaagcatt
      421 tggcctcatt aagcctagtg tcagataatg cttcaaggtc gtttttattc atacaattat
      481 aacttgaatt atcctaattt ttatgattat acccttatca ggtatgatgt aggaactgaa
      541 aacaatattg gatatcattt tcggtatttc tctgcaaatt ccagcccaca ggatctcggt
      601 gtacaatctg tcgctgatcg cgcaaattta gggtcaatca cttcattttt tgcatcaaac
      661 cctcactaac atcgaacgat tatagtacaa gtagtgttct tgacttctca ttaaaccgtc
      721 agcattcaat taattacaaa atactagcgg catttctctc ccgcaacttt attacagccg
      781 ttcagaacgt aaatgcgtca atactcgact ccacttatca tcctgccacg gccggaattt
      841 tacatgtgcc gtttctctgg caaggattgc ctgtgcctta ttgagtatcc ggggatattc
      901 ttgttcgatt gaagtgaagc gaccggcttc gcggtgttct gctacctgaa gaagcggagt
      961 gacgttttgg caagcggtta acattgtgtc acttgcccgc cataaccaag caagtgtgca
     1021 aagttcgtta tcagtgaatt gttttgtgat tggggattgt tgaatttctc gatcgagaat
     1081 atccagcacc cagcggcgga attctttggc cacaggagtg cgggcgaaca tggcgatcag
     1141 atgggcgcca cggagggaga aaatgcgagt cttcttacga taatttcctg aggtcgttga
     1201 ttcgacgacc tgagacattc cgtcagtaaa ctcatcaata tttttgttgt agatgtcggt
     1261 tactgctttg gtgctggcat attttaatgc tgccgccaat tctttgctgg taaaccagat
     1321 ctggttgttg tgatttatag gagtgaattt tacatcgcgg aatgcgagct gagtgaccat
     1381 agcgataatc tcgttttgaa tttgagctat caccaccgac gacgccaatc gactggtggt
     1441 ggactgtgca aggttggcgt aaccggtcaa aacgaaatcc ggcgcttccg aagaagccct
     1501 cacacagccc accataattt ttgtgtagac gtgcactgcg cataaaaaaa ccgcgaacgc
     1561 ggttatgcgt cgttttgatg tccgggacgc caatcccgtg tgccgatttt gcggcaacgc
     1621 acagaatata gcgccggata aataatgtcg tcaacccttg catatggggg gcgcgatgat
     1681 cactactacc accataatcg ctccaactgt tgcaactact ttaggctaag acatcacgat
     1741 ttccatatca cctttagccg ccagacaaac agagactccc tgttcttggc tgccaaaaac
     1801 accgaatgca aaaaatcgca ggtgtatttc tgcatctgtg gagggagaag agagtttgga
     1861 ttgtatgtgc gcataaacgt c
//
LOCUS       DAZKGS010000363         1878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKGS010000363 DAZKGS010000000
VERSION     DAZKGS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..571
                     /locus_tag="ACHOQY_005113"
     CDS             <1..571
                     /locus_tag="ACHOQY_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9681006.1"
                     /translation="FLSYQENNLNCYESHETLAKIFKSSASTIKRKINDLKEMGLLET
                     SPHPDPYISSLIYNALPLTDAHITPPGESSLSDLSEAEEAQEQPKGHKQPSFDVLDDD
                     WDTPLPWETEETPVSSSEKVANDNEEDVLKNFASLIIKQKNTRTGGASFIEFATSLAY
                     KHRLKLPNGIEAYFAKKHPKVYEDFDIPF"
     gene            583..861
                     /locus_tag="ACHOQY_005114"
     CDS             583..861
                     /locus_tag="ACHOQY_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681007.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIEFIYSTL"
     gene            994..1404
                     /locus_tag="ACHOQY_005115"
     CDS             994..1404
                     /locus_tag="ACHOQY_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681008.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPDFQILDNADHYGDF"
     gene            1406..1741
                     /locus_tag="ACHOQY_005116"
     CDS             1406..1741
                     /locus_tag="ACHOQY_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681009.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPASLNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGTITEE"
BASE COUNT          662 a          343 c          337 g          536 t
ORIGIN      
        1 attccttagt tatcaggaaa acaaccttaa ttgttatgaa agtcacgaaa cattagctaa
       61 gatttttaag tcttcagcgt caacaattaa acgtaagata aacgatctta aagagatggg
      121 attattagaa acatccccac atcctgatcc gtatatttca tctttgattt acaatgctct
      181 ccctctcaca gatgcacata ttactccccc aggagaatca tcactctctg atctttctga
      241 ggcagaagaa gcacaggaac aacctaaagg ccataaacag ccttcttttg atgttcttga
      301 tgatgattgg gatacgcctt taccgtggga gacggaagaa acacctgttt catcaagtga
      361 aaaggtagct aatgataatg aggaggatgt gttaaagaat tttgcctctc ttattatcaa
      421 gcagaagaat actagaacag gaggagcatc atttatagaa tttgcaacat cgttagccta
      481 taagcacagg ttaaagctac caaatggtat tgaggcatat tttgctaaga aacatcccaa
      541 agtatacgaa gactttgata ttccatttta ataaggaggg ttatgattca atatttagta
      601 aaaaatcaag tagacagaat tcagtgtaac gacacaggaa aacgcatcta cgaaacactt
      661 gcttacctct ataaaggaaa gccaacaccg ctaaaatata gcgatgtgtt acatcgagca
      721 gggtgctctg aagatggttt aaaattctgg ctgaaacagc tatcaaactt cggtgttata
      781 gaaattaaag aattatcttt ctctacattc aatctcaaaa gactagataa agaaatagaa
      841 ttcatctatt ccactctcta aaatctctct atgtaattaa aatataaaag gaaaacatta
      901 ttatgatagt agctatgtat tggttattgt tctttctcac gttagaaaca tacttcgggg
      961 tgttttcctt taaaagaaaa ggaaaagtaa ataatggcaa aaagaaaaaa caacgttgtt
     1021 aagaaaatcg gggattcagc ttctcttcta agtaaaccta aatctattct aagacgcaaa
     1081 gactttaaag agctagttac gctcaccaag caaaatagcg cccctggcga atggaaaaca
     1141 gagatcatag aacattctcc ctctgtgcct tgcggagatg aatttaacgc cttacaagaa
     1201 atcttatctt caacacctgg cgtattctgg aaacctagaa aaagaaaaga gtatatcgtt
     1261 gatagctctg atttacgcaa ataccagatt ttaggatttg aagactataa ccactacgtt
     1321 ggttatctcg ccacaaatgg cctaaataac ttagttccag acttccagat tctagataat
     1381 gctgatcact atggagactt ttaatatgaa caaaaacacg tatgacacaa tttactcact
     1441 aattaattat tatgaggatg attaccttct gcctttaaac cgcgctgaac ttgaagcata
     1501 caaagaaaac acaccagcgt cactaaatga ggcgtttaaa cactgggatt tagctgtgaa
     1561 tgcctttgag cacctctcta agcgtgtaga gatgctctgt aagcgcgaaa acgcatacct
     1621 gacagccgat cagatatggg agctatcaaa ctggatagaa gatatcgaat ctgatgttcg
     1681 ctatgtggga gatggacttg tcgaactcgc tcaacgctta ggcactatca cagaagaata
     1741 aaaaaagcaa caaaaaagtt gtaaaatatc ttgacattcc atctattata tgttaatata
     1801 attatatggg gtgattattt ttcctttatt ggttcgatat taaaaattct tcttgtcgtt
     1861 aaaggggagt aaaatctc
//
LOCUS       DAZKGS010000364         1868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKGS010000364 DAZKGS010000000
VERSION     DAZKGS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(510..692)
                     /locus_tag="ACHOQY_005117"
     CDS             complement(510..692)
                     /locus_tag="ACHOQY_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9681010.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            complement(689..1216)
                     /locus_tag="ACHOQY_005118"
     CDS             complement(689..1216)
                     /locus_tag="ACHOQY_005118"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9681011.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(1213..1659)
                     /locus_tag="ACHOQY_005119"
     CDS             complement(1213..1659)
                     /locus_tag="ACHOQY_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9681012.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            complement(1667..>1868)
                     /locus_tag="ACHOQY_005120"
     CDS             complement(1667..>1868)
                     /locus_tag="ACHOQY_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681013.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
BASE COUNT          493 a          498 c          362 g          515 t
ORIGIN      
        1 tgttagactt taactctata ttgatataac attagccaaa tattcaacag tgtatgcgac
       61 caaacaccaa catgtcgcat acatatacaa tttgaatatt taattatatt tacttaatgt
      121 atttctataa aagcaaatta caaactcaca acaaaaaacc tcataacaca ttaacaatca
      181 attcttttca tctttataaa ttctcatatc aggcttgcac ccgataaacc gacgaaaact
      241 atttaaaacc catcgagtga agtaatctct aaaaccaaag aaataccaag tgaaaatatt
      301 cacgataaaa tgcccggtca aagcccctcc tgtaccgcat gcaagaacag taaaaaatca
      361 gatgttttca taaatatcag tcctcatcgt tttgcctggc atgtccttta ccagcaatct
      421 tctgtatgca ctaagcctag atagaatcca ctcagtgtac actgaagccc gctcgacgct
      481 ttcttgttcg taacttcgat tttagtcaat taccttgttt tcctcgcacg atgtcttagc
      541 caccggatat cccacaggtg agccgtgtaa ttgaaggttt ttacgtcaga ttcttttggg
      601 attggcttgc gtttatttct gtagcgtttc gttggaaggt atttgcagtt ttcgcagatg
      661 atgtcggtga aacttcgtcg ctgtcgcctc atgccgccct cctgacgccc tgcccgatcg
      721 ccatcaatgc cgctttggat acggtagtaa acatccgtcg aggactgatg aacggtcgcc
      781 aaatcagcag catggagcct ttactgtttc ccttcttctc cagccctgtc gatggttcga
      841 taaaattaat ccgtccatca gtgataatgc gaacttcgtc aacactctcc agagccttgc
      901 tgaaccatcc gacagacata tcctctggca caagcatcac taccgtctgt cgctgttgta
      961 tgcactgctc agcggctttt tccacccacg gcctgatatt gctgtacggt gggttattcc
     1021 agattgcacc gtggcttatc cactcagaat tgagtgcgtc gtcggcctca gttagccagt
     1081 gagcgcacag agcatttttg tcgctcgcag ctgaatccag ccagaatcca aactcaatat
     1141 ccagcgcatc aaaaagccaa agcggcgttt gccagcagtc cttgtcgtgt gctggtgtat
     1201 ttgatttgat agtcatgcag ccctaccttt tcgttgtgac cattcatact ctcgccggga
     1261 gtcatcactc caccgcacgt tgcgctctga gccgaaccaa aacatgattt cgataagctc
     1321 agtcatgctg gcctttcgca ttttgctggt acgcacgcca agcatgacaa cgccaccatc
     1381 gataccaggc acacttcgtt gctccagttt tttggtctta agccacaggg cagtgaacag
     1441 gtctttccag tcctccggcg caagtctctg tccatgccaa agcacctgac gtgatacgtc
     1501 ctgcaataac gcccacataa ggcggttttg aggattgctc cgctttggtt ctttaatgtg
     1561 gacttcgtga ggtgacttgt cgtcgatcgg aagtgagagt attgcgtcta tggcgttgtt
     1621 tctgattgct tcgttgcgaa gcatgtatat ttgcttcatt gtcacctcaa ctcacaaaac
     1681 gccacgccat ttttgctaca gcgacaggcg caacaccgat aatcacccac aggaaaatgc
     1741 taccgaaaag cacaccaacc aggtctttac cttcgcctac cagccggaca aaactgctgg
     1801 caaccacaat gaacgtcgcc accatccaca tagcaccgag aatcctcaat gcagaaaaaa
     1861 tcaactca
//
LOCUS       DAZKGS010000365         1866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKGS010000365 DAZKGS010000000
VERSION     DAZKGS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..1717)
                     /locus_tag="ACHOQY_005121"
     CDS             complement(65..1717)
                     /locus_tag="ACHOQY_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9681014.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT          555 a          475 c          397 g          439 t
ORIGIN      
        1 taaacgatgc tggaagtatt actctcagcc atcatttaac tccctattac tggtagcgat
       61 aaaattatcg gctatccatg attaacgttt ccagcacatc taattcactg actatttttg
      121 ccagtgtttc atcactgact tcttgccgtg cacgcatctg gtaaatagcc gcacgcctgg
      181 catttaacgc tgccagatta aatgttcttt ccagcttcgc gcgctgcata tgtaaagaat
      241 tatcattttc cgacatatca tcttcgctac gccgcatata accgataact ctggcccctg
      301 tttccataat caactcgttg tcgaggtttt ctgtactggt gcgtagcaaa cgttgctcag
      361 tcttttgcac actgagtatg gccattttgg ccataaacag atcggcagct tcctcttctt
      421 tttgccggtt atcgctgaaa atgcccacat ttttccccag tagtaacggc agaataataa
      481 tgccgcaaac cagcgaaaag aggatcacac ctgtggcgag aaataccagt aagtaacgcc
      541 cgggaaaagg tgtgccatcc ggcagcaata acgggataga aagcaccccc gcaagcgtga
      601 tggctccgcg cacccccgca aaggtcgcca gcagcatatg acgcgtttta tatccagcaa
      661 acagcatcgg atgtttgccg ggtcgtttac ggctatacag acgcatcagc caaagccagc
      721 ccaggcgcac aaccattaac gccaggtaga tccaaatgac atcaaggagc agcgatccgg
      781 tgtctaccag tggatcgttt tctgcacgga tcagtgattc cgaaataatg cccggcagtt
      841 gtaagcctaa aatcagaaac accatgccgt taaagacata ttcaagcatc gaccagctac
      901 ttttagcttt aattctcagg cggataggcg cgtggtggac tacgccaatg cgactggttg
      961 ttaaccccgc ggcgacagcg gcaagaatgc cagataagcc aaaatgttcc gcaaccagat
     1021 aaacaataaa cggcaacaga agcatcaata caatctgaat ggaaggttca tcgtgagcat
     1081 atcgactgat caacagcagc cctttgccat ataaccaggt aacagcaatc cccacggcaa
     1141 taccgcccaa cgcaacgaca aaaaacgcaa tggttgcgcc atgaacgctg aattccatcg
     1201 ttcccatcgt cactgcaaca gcaaatttca acgccaccag gccggaagca tcattcatca
     1261 acgcttcgcc ttcgagcact tcctggatgt ttttgctgat acgccctttc ccgacaatcc
     1321 ccagcaacgc cacggcatcg gtaggcgaca acacggcggc taatgcgaaa gcagcgatga
     1381 gcggcacatt cggtaagagt aaatgaatca catagccgac accaaaaata gtgattagca
     1441 ccaacacaag cgccaggctg ataatttccc gactgtgctt gatcaattct ttaggttgta
     1501 tttttgagct ttcagcaaac aacaaggggg gtataaacaa taagagaaaa agttcaggat
     1561 tgaattccac atgcagacca aatatgggct gggccagtac agcgccagcg gcaatctgga
     1621 tcaaaggaag tggtatctga acaggagaca gacgcgtaag cacgctggtt aatgatacta
     1681 ccaacgtaag aattaaaatt gttaaaagta tttccatata ataaacaggc ttcctcattc
     1741 ataaataatg ggtattatta atttaacttc ataaaaagcg ccctggttag cacttaacgt
     1801 taatcattaa tcacagcgtt cactcacatg ctcaggagga aaccagtgag atgagttttc
     1861 cagttg
//
LOCUS       DAZKGS010000366         1866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKGS010000366 DAZKGS010000000
VERSION     DAZKGS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..463)
                     /locus_tag="ACHOQY_005122"
     CDS             complement(<1..463)
                     /locus_tag="ACHOQY_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9681015.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIA"
     gene            732..848
                     /locus_tag="ACHOQY_005123"
                     /pseudo
     CDS             732..848
                     /locus_tag="ACHOQY_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(953..1029)
                     /locus_tag="ACHOQY_005124"
     tRNA            complement(953..1029)
                     /locus_tag="ACHOQY_005124"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(993..995),aa:Arg,seq:tct)
     gene            complement(1043..1119)
                     /locus_tag="ACHOQY_005125"
     tRNA            complement(1043..1119)
                     /locus_tag="ACHOQY_005125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Arg,seq:tcg)
     gene            complement(1127..1202)
                     /locus_tag="ACHOQY_005126"
     tRNA            complement(1127..1202)
                     /locus_tag="ACHOQY_005126"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1167..1169),aa:Ile,seq:cat)
     gene            complement(1231..>1866)
                     /locus_tag="ACHOQY_005127"
     CDS             complement(1231..>1866)
                     /locus_tag="ACHOQY_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9681016.1"
                     /translation="PGSVVLATKCIRALFLMHYGKVKIFDINHSIVSQYLEIQHKLTR
                     THLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSG
                     CLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMR
                     VAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          485 a          485 c          448 g          448 t
ORIGIN      
        1 tggcaatatg taagccctgg ccgacacagc cgtactgccc tttgctcagg tctgccttcg
       61 gatgattcag cgtactcata tcctgcacat catgcaggca gtggtcagcc ggaatgatgt
      121 cgttatacgt acaactctct ccacccggag ttaccgtgct gcggcgcgcc agctgtttaa
      181 tgcgcggatc cggagcatcg taagaatccg gtaacggaag cccttcaccg taggccatgc
      241 cgttggactg tccggcaagc acaaccacgt agaaccagtc cggctcagat gaagggccga
      301 cctgtggatc tccttcaata gccaccgcct gcatcagtgt gtacggcgta atggcaaccg
      361 gtccgccgta tggctgccag ccctctttca gtttgtgtgt cagcttttcc gcaaggtctg
      421 acggcgacgc cgccctgaca acatcatagt gtttaaatgc catgaatcct cccggccggg
      481 ataatattgt gagtaaaatg aggagcgggc tgaagtccgg aagttacagg acaatggcag
      541 aagagagaca acagcccgca atacgaaaaa ggccgcgcta ttgcgcagag tgattactgt
      601 cggatattat tcgccagctg aaatattact tcacgttttg ttgtttattc cttgccgccc
      661 gcgtctccct gcgcgggctt tttttgtcca taagaaagcc cctccggaga ggggctggag
      721 agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca gtaccagcac
      781 ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac agggggattc
      841 accatgccag tttcttttaa caaactcccc gcaaaccaga caacagtcaa ccgcctgaat
      901 tgtgagacat ttaaaaaaaa ggcccgcaaa agcgagccag ggaaaataag tgtggcgcgt
      961 tgtactggat tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc
     1021 taacaacgca ggatacagat aatggaccgc cttcggggac ccgaactccg cgcaaccagc
     1081 ttcgaaagct ggcgctcttt cctgatgagc taatggcggt atgtgatggt ggcccttgct
     1141 ggatttgaac cagcgacctg gcgattatga gtcgctcgct ctcaccactg agctaaaggg
     1201 ccgggtcaaa aaataataat cagatgaaaa tcaataatca agcccttgcc tggatacata
     1261 tctgtctggc gggaagccat aatagcggtg aaatacagaa ataaagtagg accagcttga
     1321 ataaccgcat ttttctgcta cagcctgtcc atatccatgc cgggaacata acatattcac
     1381 agcaacacgc atccgctctt ccagcaacaa gcgactgaac atgtgccctt catttttcag
     1441 ttttatcttt aacaaactct cactcatatg caggcgtaac gcaatcgcac caagcgtcca
     1501 gcttgctgat atatctgtct gaattatcgc cctgactttg gcacttatac tggataaaca
     1561 tccacttaaa aataataata tccgttcatc tgattcaaac agcgacaggc atgccatcat
     1621 aagaaacata tccgtggtat ctccggaaaa tctctggctg gtaattaaag cctcagccaa
     1681 cgcagggttg ttgggttcca gtgacagata aagcggaacg tcagtcagat gagttcttgt
     1741 cagcttatgc tgaatttcca gatattgact tactatggaa tggtttatat cgaaaatttt
     1801 aactttgcca taatgcataa ggaaaagcgc cctgatgcat ttggtggcca gaacgactga
     1861 gccggg
//
LOCUS       DAZKGS010000367         1858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKGS010000367 DAZKGS010000000
VERSION     DAZKGS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHOQY_005128"
     CDS             222..1169
                     /gene="stxA1a"
                     /locus_tag="ACHOQY_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9681017.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1179..1448
                     /gene="stxB1a"
                     /locus_tag="ACHOQY_005129"
     CDS             1179..1448
                     /gene="stxB1a"
                     /locus_tag="ACHOQY_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9681018.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          498 a          328 c          456 g          576 t
ORIGIN      
        1 gtaacatcct gtgtatcaat aaatgttgtt atctacgtac gtcaagtagt cgcatgagat
       61 ctgaccagat atgttaaggt tgcagctctc tttgaatatg attatcattt tcattacgtt
      121 attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg aggatgtcaa
      181 gaatatagtt atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta
      241 gagtgctaac ttttttcttt gttatctttt cagttaatgt ggtggcgaag gaatttacct
      301 tagacttctc gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag
      361 gtactccatt acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggct
      421 caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta
      481 ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga
      541 caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcca ggtacaacag
      601 cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgca gggatcagtc
      661 gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat ttaatgtcgc
      721 atagtggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt gttactgtga
      781 cagctgaagc tttacgtttt cggcaaatac agaggggatt tcgtacaaca ctggatgatc
      841 tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacattg aactggggaa
      901 ggttgagtag cgtcctgcct gactatcatg gacaagactc tgttcgtgta ggaagaattt
      961 cttttggaag cattaatgca attctgggaa gcgtggcatt aatactgaat tgtcatcatc
     1021 atgcatcgcg agttgccaga atggcatctg atgagtttcc ttctatgtgt ccggcagatg
     1081 gaagagtccg tgggattacg cacaataaaa tattgtggga ttcatccact ctgggggcaa
     1141 ttctgatgcg cagaactatt agcagttgag ggggtaaaat gaaaaaaaca ttattaatag
     1201 ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac gcctgattgt gtaactggaa
     1261 aggtggagta tacaaaatat aatgatgacg atacctttac agttaaagtg ggtgataaag
     1321 aattatttac caacagatgg aatcttcagt ctcttcttct cagtgcgcaa attacgggga
     1381 tgactgtaac cattaaaact aatgcctgtc ataatggagg gggattcagc gaagttattt
     1441 ttcgttgact cagaatagct cagtgaaaat agcaggcgga gattcataaa tgttaaatac
     1501 atctcaattc agtcagttgt tgccggtctg ataatagatg tgttagaaaa tttctgcatg
     1561 gtgaatcccc ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa
     1621 gcgagaatac gcgggtttgg tggcaccagg ccgaactcac cgggaggcac ccggcatcat
     1681 gctgtataca gagattaggc atatatccag gctcctcatc gcaggagcct ttttacatgc
     1741 aaaaaaaagc ccgagtgggt tcgggcaaca gcatgagata cttgcattgt catttttatc
     1801 gtgcggattt taaccaggat tcataaggct gcgcaactgc gcggcctttt tcgtattt
//
LOCUS       DAZKGS010000368         1855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKGS010000368 DAZKGS010000000
VERSION     DAZKGS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..617
                     /locus_tag="ACHOQY_005130"
     CDS             45..617
                     /locus_tag="ACHOQY_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9681019.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            678..>1855
                     /locus_tag="ACHOQY_005131"
     CDS             678..>1855
                     /locus_tag="ACHOQY_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681020.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     "
BASE COUNT          405 a          483 c          592 g          375 t
ORIGIN      
        1 tttacgggga tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg
       61 aagccatccg cgcactggcc acacagctcc cggcgtttcg tcagaaactg agcgacggct
      121 ggtatcaggt acggattgcc gggcgggacg tcagcgcgtc cgggttaacg gcgcagttac
      181 atgagactct gcctgatggc gctgtgattc atattgttcc cagagtcgcc ggggccaagt
      241 caggtggcgt attccagatt gtcctggggg ctgcagccat tgccggatca ttctttaccg
      301 ccggagccac ccttgcagca tggggggcag ccattggggc cggtggtatg accggcatcc
      361 tgttttctct cggtgccagt atggtgctcg gtggtgtggc gcagatgctg gcaccgaaag
      421 ccagaactcc ccgtacacag acaacggata acggtaagca gaacacctat ttctcctcac
      481 tggataacat ggttgcccag ggcaatgttc tgcctgttct gtacggggaa atgcgcgtgg
      541 ggtctcgcgt ggtttctcag gagatcagca cggcagacga aggggacggt ggtcaggttg
      601 tggtgattgg tcgctgatgc aaaatgtttt atgtgaaacc gcctgcgggc ggttttgtcg
      661 tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga
      721 aagataacct gaaatccacg cagctgctga gtgtgattga tgccatcagc gaagggccga
      781 ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg gtgctggaca
      841 gtgaggggaa taccaacatc tccggcgtca cggtggtgtt ccgggcaggt gagcaggagc
      901 agacaccgcc ggaaggtttt gaatcctccg gctccgagac ggtgctgggt acggaagtga
      961 aatatgacac gccgatcacc cgcaccatca cgtcggcaaa catcgaccgt ctgcgcttta
     1021 ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag
     1081 tccgcctgct ggttcagata cagcgtaacg gtggctgggt gacggaaaaa gacatcacca
     1141 ttaagggcaa aaccacctcg cagtatctgg cctcggtggt ggtggataac ctgcctccgc
     1201 gcccgtttaa tatccggatg cgcaggatga cgccggacag caccacagac cagctgcaga
     1261 acaaaacgct ctggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca
     1321 cggcactggt cggcgtacag gtggactcgg agcagttcgg cagccagcag gtgagtcgta
     1381 attatcatct tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg cagacgcgac
     1441 aatacagcgg tatctgggac ggaacgttaa aaccagcata cagcaacaac atggcctggt
     1501 gtctgtggga tatgctgacc cacccgcgct acggcatggg gaaacgtctt ggtgcggcgg
     1561 atgtggacaa atgggcgctg tatgtcatcg gccagtactg cgaccagtca gtgccggacg
     1621 gctttggcgg cacggagctg cgcatcacct gtaatgcgta cctgaccaca cagcgcaagg
     1681 cgtgggatgt gctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacgggc
     1741 agacgctgac gttcgtgcag gaccgaccgt cggataaggt gtggacctat aaccgcagta
     1801 atgtggtgat gccggatgat ggcgcgccgt tccgctacag cttcagcgcc ctgaa
//
LOCUS       DAZKGS010000369         1849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKGS010000369 DAZKGS010000000
VERSION     DAZKGS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..681)
                     /gene="nleH1-2"
                     /locus_tag="ACHOQY_005132"
     CDS             complement(<1..681)
                     /gene="nleH1-2"
                     /locus_tag="ACHOQY_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFP9681021.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDNGNVIGIRMNKINGES
                     LLDIPSLPA"
     gene            complement(788..859)
                     /locus_tag="ACHOQY_005133"
                     /pseudo
     CDS             complement(788..859)
                     /locus_tag="ACHOQY_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            853..1847
                     /locus_tag="ACHOQY_005134"
                     /pseudo
     CDS             853..1847
                     /locus_tag="ACHOQY_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          555 a          444 c          389 g          461 t
ORIGIN      
        1 tgctggtaat gatggaatat ccaaaagaga ttccccattt attttattca ttctaatacc
       61 aataacattt ccattatcat tatatatttt ctctgcactc ccggaaccat aatactgatt
      121 gaaacaacga acttcgcttg tcacctcttc atggctttga gatatagtaa acatcttcaa
      181 cacttttgtt gtatcttcca tatcttcata cacgacagca ttaccacctt taccaataac
      241 attacctggc acgggtgatt tattgttgct aaccctcggc aactctgatt gagtataatc
      301 cgctggaggt aactccggct gcgcataatc cactggaggt aaatcaggtc tgttcgaatg
      361 aacagagcct ctttctatag tcgtgctcac tggcgatgta ttcagcatag cctcaatttt
      421 tctgctaatc tctcccttag gccatcccaa cctgtgcaac agattagtaa aacaaccact
      481 atgactttct cttgtaacgc aaaacttatt atcagtgacc acaacacgat atgttctgtt
      541 gccaaccgtt acttgcgtcc cgctatcaga gtgaacagca gcatccctta cagaggataa
      601 aacacgatta tcaggcgaag tcaggtttct ggttaaagaa ttccatgaac atcccaaatt
      661 tatagaagag ggcgataaca tacatttcaa ccttcaaaat aaacctatct aattattcct
      721 aacagacatc ccccatacat gacaacaaaa accggagccg gactccggtt tttgtgaagc
      781 tatcgggtta cttcatttcg ccaatatttt cccacttccc gtcagcacgc aggatttgca
      841 gcggtcttac tacacactga cccgaggacg aaaaaagagt tcgggctggg aagagacaga
      901 agagtagcaa tttcagaagc attcacacca acattgagct tttcagcgaa agtgggcacg
      961 aatcattgct ggacagggtt aaaggcactg actcaatcac tcttcatacg tggcttgatc
     1021 gatatgaaac aatcctcagc tagaggggga gcaagccgaa aacattactc gactacgcca
     1081 gcaaaatcag ggcaattcga agaaaattgc cggacaaacc gctcactgac atatcaacga
     1141 aagaggtggc agcaatgcta aacacctacg tcgcagaagg taaagcggtt tccgcaagag
     1201 taatcaggtc aacccttgtt gacgtttttc gaggggcaat agccgagggg catgtggcaa
     1261 cgaatccagt aacaacaacc cgtgcagcaa agtcagaagt aaggcgctca aggctgacag
     1321 ctaatgagta tgtcgctatt taccatgctg ctgagcacct ccccatctgg ctgaggctgt
     1381 caatggattt agctgtcgtt acagggcaga gagtgggcga tttgtgcaga atgaagtggt
     1441 cagacataaa cgatggtcat cttcacattg gacagagtaa aacaggagcc aaaattgcca
     1501 ttccgctggc tctaaccatt gacgcactcg acatctcact ggttgataca ctacagaaat
     1561 gcagggaggc cagcagcagt gaaacaataa tcgcatcaac ctatcacgaa ccactttctc
     1621 cagccacagt atcacggtat ttaacaaagg cgcgaaatgc atccgggatc tcgtttgatg
     1681 gagacccacc gacatttcat gaactacgta gtctgtccgc gaggctatat cggaaccaga
     1741 ttggctacaa gtttgcacaa cgtcttcttg gacataaatc tgattcaatg gcggcgcatt
     1801 atagggacag tcgcgggcgg gagtgggaca aaattgaaat cggataatg
//
LOCUS       DAZKGS010000370         1834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKGS010000370 DAZKGS010000000
VERSION     DAZKGS010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            200..466
                     /locus_tag="ACHOQY_005135"
     CDS             200..466
                     /locus_tag="ACHOQY_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9681022.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            463..1062
                     /locus_tag="ACHOQY_005136"
     CDS             463..1062
                     /locus_tag="ACHOQY_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9681023.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1055..1342
                     /locus_tag="ACHOQY_005137"
     CDS             1055..1342
                     /locus_tag="ACHOQY_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFP9681024.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1335..1790
                     /locus_tag="ACHOQY_005138"
     CDS             1335..1790
                     /locus_tag="ACHOQY_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681025.1"
                     /translation="MFDFPRPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQEPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
BASE COUNT          385 a          525 c          485 g          439 t
ORIGIN      
        1 tttcactata attttttcca gctccttcca gagcgtttta aagctatctg gcgctgatgt
       61 acaaaaaata cacagccatt gtaaggctgc cggaacaaat tccccctgtt gcgtctgccg
      121 aaaatattca caaaataaag cgctacccga agccggacgg acttatccgg tgctgtatgg
      181 acattaacga ggtagcccga tgcaagctgt tttttcttcc ccgtctcccg ccccagtgac
      241 gccactgatg ccgctgccgg acatcacgca ggagcgtttt ttacgtctgc cggaagtgat
      301 gcacctgtgc ggcctgtcac gctcgaccat ctatgaactt atccgtaagg gggaatttcc
      361 gccgcaggtg agtcttggcg gtaaaaatgt ggcctggctg cactctgaag tcaccgcatg
      421 gatggccggg cgcattgccg gacgcaaacg ggggtacgac gcatgatgat gcccgctctg
      481 caaaaactcc ctttttctgg cttgcctttt tccggcattt gcggatatag tttttccgct
      541 gccgcaaaat cggcagccgg gcgtgagaac ccgagcaatt cacaggcgac atatgacgcg
      601 ccacgcgtct ttttttgtgt cgcaatcaat gccacagagc gccagattat ggtgtggtgt
      661 gtggtcagtc gtgcagatgg cattccgtgc atcctgcccg cgtccgctca ttatgccgca
      721 gagtcaatgg tagctcaggc ggggcagcct ccgggctggc cggtatcctg tgaagccggt
      781 attctcaccc ccgtctgggc tatcgccatc gagcgtgaga actccggcga tagcgttatt
      841 tgctattcac aggaggctgc catcatggct acaaccctta ccccctcaca ccctgaattt
      901 gtctttgtgt ttgcggctgt ccgtcgcgca gaccgtcatc cccgtatctg tatgcttcgc
      961 accgtcgccg gtgatgaacg cagtgcccgc cgttcccttg tccgtgacta tgtgctctcc
     1021 cttgctgccc gtctgccggt ggtggaggtg tcccgtgcgt aataaaaaag cccctcagac
     1081 cgtctcagca cgtcatgacg cccgtgaaca cctcagcatt gaggcttacc ataagctcaa
     1141 ccgcgccagc gccgtatccc agtttgttgg gggtgatttg attcaccgtg aactctccgg
     1201 cctgcatcag ctctacattc cgcatatttt cagctacctg aatgaagata ttgattttgt
     1261 gctgaatgag ctgaaagcca aaggcctgtg ccgcgatttt ctcgcccagc agaaagaccg
     1321 gggagacagg acgcatgttt gattttcccc ggcccggtga gatttaccgt tctgccggtt
     1381 ttcccgatgt ggccgtggtc ggcattctgg aagacggtat tccgtgggaa atgccgtacc
     1441 gctgcccgga aattgtctgg aacccgtacc gccgtaaatt cagtatcctt gtgcgtatcc
     1501 tcgctgacgg gcgcaccaca gacatcccgc tggggcgttt tctgcgggaa tttacctgtg
     1561 accgtcctga cctgtttaaa cgcagccccg taaaccggca tgcggtactg aaagaaatgg
     1621 ccggagaccc ggaattacag aaatggcggg agaaatatct ggatatttac ccgcaggagc
     1681 ctgttccggt cagccgggcg gcaccggtgg cgcgggaatg gcgggaaatt ccccgcacgg
     1741 agcctgaccc ggaaatcacc ccggataaca gttaccgcaa ttatctgtaa ttaaaaaacg
     1801 acacccgaaa aaaaaattaa atgtgcgtat tcgc
//
LOCUS       DAZKGS010000371         1830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKGS010000371 DAZKGS010000000
VERSION     DAZKGS010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            141..644
                     /gene="ftnB"
                     /locus_tag="ACHOQY_005139"
     CDS             141..644
                     /gene="ftnB"
                     /locus_tag="ACHOQY_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9681026.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHOQY_005140"
     CDS             complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHOQY_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9681027.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHOQY_005141"
     CDS             complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHOQY_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9681028.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1438..1761
                     /gene="yecR"
                     /locus_tag="ACHOQY_005142"
     CDS             1438..1761
                     /gene="yecR"
                     /locus_tag="ACHOQY_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9681029.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          474 a          432 c          406 g          518 t
ORIGIN      
        1 tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa
       61 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
      121 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
      181 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
      241 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
      301 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
      361 atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
      421 acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg
      481 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc
      541 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga
      601 ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa
      661 tgcattgcgc cgatgaaggt tttgagaaac cgcagcctca tctgtttgaa gcagcggttt
      721 ttttaatggg attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg
      781 attgcctgca tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc
      841 agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga
      901 atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc
      961 agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc
     1021 acatcttcag cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag
     1081 gttttttatt atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta
     1141 ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata
     1201 caggtgttat tctctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt
     1261 gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc
     1321 cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     1381 ttgtcgcagc gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg
     1441 agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccag
     1501 gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct
     1561 tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc
     1621 cagcaggaag gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt
     1681 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     1741 gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa
     1801 atagcggtaa tgcgttttat tcccattcgt
//
LOCUS       DAZKGS010000372         1815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKGS010000372 DAZKGS010000000
VERSION     DAZKGS010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..612)
                     /locus_tag="ACHOQY_005143"
                     /pseudo
     CDS             complement(1..612)
                     /locus_tag="ACHOQY_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(572..691)
                     /locus_tag="ACHOQY_005144"
                     /pseudo
     CDS             complement(572..691)
                     /locus_tag="ACHOQY_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(704..>1815)
                     /locus_tag="ACHOQY_005145"
     CDS             complement(704..>1815)
                     /locus_tag="ACHOQY_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFP9681030.1"
                     /translation="VISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHK
                     QNDALGLYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEE
                     DARLNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDK
                     GYERITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKK
                     LAGPYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKS
                     AAIVYKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGT
                     LHEAPSPIIPLNWSNGSMTLMLKEMSNLINDEGIK"
BASE COUNT          487 a          396 c          312 g          620 t
ORIGIN      
        1 tttgtattat aaataaccgc ataagccaaa ttatttgaga ttaataaaac atcaccaact
       61 tcaatatcaa caagacttct gtaacatgtt ttactaaatc caattacaaa atgaatggac
      121 caatataatg attttttgtc taacactgga gcgtttccgt attggcagac tgtgacttta
      181 tctaaccaaa gattcaattc ccctgttcat attcttatta gtggttgagc agggctcaat
      241 actccatcaa atactttacc tacgtgcaaa cgattatatt ttagttcagg atgctttaga
      301 tttaacgggg aactggataa actgagccat tcccctatta acctttccgt ttctggcatt
      361 aaagccatcc cagcgagctc agatgaaatt aagggaaata tttctccaag caatacccat
      421 ccagaccagc tgccactctc ttctgctatc ggtaaccatt tagcactaaa ttcgggcatt
      481 aataatgcaa catcttcgcc attttctttc caattttgaa aagtggtttc aaacgtatga
      541 gtgtttcgat ttactttcct cagaccgaac attaactttc atccttctga tcgttttcga
      601 atttgctttc attttcttgc tcctcataag gctgatgtcg ttgtccttgt cgttcatctt
      661 gctcagtcaa tacccaacgt tgtgctgtaa ctcattatga aaactattta attccctcat
      721 cattgataag gttggacatc tctttcaaca tgagagtcat tgaaccattc gaccaattta
      781 gcggaatgat agggcttggc gcttcatgca aagttcccga cttgtggata tagttatagc
      841 tttcgggaag tgccatttca ggtacactcc tgccatttgc accaatcata ttctcgcctg
      901 ttatttgggc caatgatcta ttcataaatt gcactgagtc attcaaatac tcttcctttc
      961 ttgattcttt ataaacaatt gctgctgatt ttgcatacca tgagtcaaaa aaccattctg
     1021 cttctgttga tggaatgaag ctcttttctc ttttcgcatg tgaattttga tcggtatcgg
     1081 ttttaatatc attgaaccaa aaatttgccg actgataatt atctttctcg tatcttttta
     1141 taccatacgg acctgctagt tttttaacta tttttagaac ctgctctttt cttctggtgt
     1201 ttatctttgc aagatttgca ggataaataa cattaagtaa agccgcatcg gcttctctgt
     1261 aatgtttatc tttttccaga taacctgggg attctccccc taaatcaagc tgtaacgtta
     1321 ttctttcata acctttatct atgaggtggt tcaacctcgt tgttgaaaga ggttcatcca
     1381 gttcattggc tttagcttcg cgtagtaaat cagagacaaa aacagagtct ttcttcgaga
     1441 gtaagttcga caaacgttcc agtccagagg tcaccagtgc aacggaagat gtgtttaatc
     1501 gggcatcttc ttcccatgct ccggaatctt ccataacata gaaattggct ttatccagat
     1561 aagcaatcag cagcgcaacc gattttaatc ggtcaccttt ttgccaatct tcagcgttaa
     1621 ttgtatcagt gtcgattgct tgtataagga gatcaagata aagacctaac gcatcatttt
     1681 gtttatggtt ccacagctgc ggcttgcctt cttcctgcac atctgccatc acgggggagt
     1741 tgctgtcaaa tcggatatgc acaacattca tttgcccggg aactccatcc agtcgtttgg
     1801 ggttgctaat gacgt
//
LOCUS       DAZKGS010000373         1812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKGS010000373 DAZKGS010000000
VERSION     DAZKGS010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..349
                     /locus_tag="ACHOQY_005146"
     CDS             47..349
                     /locus_tag="ACHOQY_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFP9681031.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMTPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            433..810
                     /gene="sctI"
                     /locus_tag="ACHOQY_005147"
     CDS             433..810
                     /gene="sctI"
                     /locus_tag="ACHOQY_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9681032.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSIQ"
     gene            812..1384
                     /gene="escJ"
                     /locus_tag="ACHOQY_005148"
     CDS             812..1384
                     /gene="escJ"
                     /locus_tag="ACHOQY_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP9681033.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1390..>1812
                     /gene="sepD"
                     /locus_tag="ACHOQY_005149"
     CDS             1390..>1812
                     /gene="sepD"
                     /locus_tag="ACHOQY_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP9681034.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPT"
BASE COUNT          626 a          342 c          342 g          502 t
ORIGIN      
        1 attgataacc agacaaatgt ctaaattaga caatggagaa aagttaatgg atgcagcaaa
       61 tttaagtcct tctggcggag tattaccact ggcagccaca atcaatggaa atgtttcggt
      121 ggatgaaaat acaggagtaa tgacaccaga aaatggggta agtcgtaata tcagaattgc
      181 agccggaatt gcactcgcta ctacagctct ggctgcttta ggtactggta tcgcaatggc
      241 atgcacaaca gatgcaagct cccaagaatt cttaggttta ggtattgcta ccggtgtatt
      301 aggtggtgtt actgcgcttg gcggcggatt agcgatgaaa tatgcctaat catcgcatcg
      361 cataaataaa atagagaggt catggatgca ttatgccatt acctctctca tgaaaagaga
      421 ctaaccgtaa atatgaacaa tattaatcaa tcagaaaata ttaatattca ttttaataaa
      481 gtatcccaaa caaatttcgt tgatgaccat accccgttag caccaacgcc ctctgcggct
      541 ggagcggctc agtttcttga tcaattactt cctaaaacag ctggtgtatc ttctccagag
      601 caagttttaa ttgaagaaat taaaaagaga catcttgcaa caatgaacac cgatctcagt
      661 ttcgatgccc tatctgcagg tggactctcg ccagaagatg tgctcacatt acaaaagaat
      721 gtgctcaacg caaacgtcaa tgttgatgtc gtatccaagt tagcaagtct tctttcaaca
      781 tcggttacaa aactggtttc aatacaataa aatgagaaaa tacattaaaa acaccttttt
      841 atttgttttt atttgtttga cagttgcatg taaagagcaa ttgtatacag gattaacgga
      901 aaaagaagcc aatcagatgc aagcattgtt gttatccaat gatgtcaatg tatctaaaga
      961 aatggacaaa agtggaaatg taacaataag tgtggaaaaa gaggattttg tccgtgcgat
     1021 aaccatttta aataacaatg gcttcccgaa aaagaaattt gcagatattg aagttatttt
     1081 tccgcccagt cagctagtgg catcgccatc acaagaaaat gcaaaaatca actatctcaa
     1141 agaacaagat atagaaaggt tactaagtaa aatccccggc gttatcgatt gcagcgtttc
     1201 attaaatgtt aacaataacg agagccaacc atcctcagcg gctgttttag tcattagctc
     1261 gccagaggtt aacttagcac catcagtcat tcagataaaa aacttagtta agaatagcgt
     1321 agacgatctc aaactagaaa atattagtgt cgttatcaag tcatcctcag gacaggacgg
     1381 gtaatatgta tgaacaataa taatggcatt gttaagaatg atagtgactg gctaacggct
     1441 cttgattttg taaaagatgt caaaggttca ccaaagcatc ttacttttca tatctatcaa
     1501 aaaaatgcct tcttacacga ctttggaagt tactgggttc tgtatatcga gcttaatggc
     1561 gattttcgcc aggttccaac agataccttc atcagactct gtaatattct ggccgttagt
     1621 aaagaatata aacaaatggg catttttcaa tctaataaaa aatggtatct ttgccaactg
     1681 tttaataaag ataataatca tcgcgcaaac atgtcaaaag caataatgca acacacactc
     1741 gcctctttgc ttgacaagca atttgataag ttagagcaag catctacatc agatacaacg
     1801 atgcctccaa ca
//
LOCUS       DAZKGS010000374         1810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKGS010000374 DAZKGS010000000
VERSION     DAZKGS010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..583
                     /locus_tag="ACHOQY_005150"
     CDS             <1..583
                     /locus_tag="ACHOQY_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFP9681035.1"
                     /translation="IKTEQTGKLLNRKITTTPTPAKLLSYRNADLIKENYITEKVLSI
                     FNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFGIMMGTPRRSAD
                     IAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSASVGSLLDKGAKV
                     YPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(596..817)
                     /locus_tag="ACHOQY_005151"
     CDS             complement(596..817)
                     /locus_tag="ACHOQY_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681036.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(901..1281)
                     /locus_tag="ACHOQY_005152"
     CDS             complement(901..1281)
                     /locus_tag="ACHOQY_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681037.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1482..>1810)
                     /locus_tag="ACHOQY_005153"
     CDS             complement(1482..>1810)
                     /locus_tag="ACHOQY_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9681038.1"
                     /translation="DSGEAQSQESIQDKISQCKFPVSSGNFQCPPESIQCPITLERPE
                     EGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHPLTRAPITPSMIVKPEECKYDPARGS
                     FIIKDS"
BASE COUNT          599 a          380 c          310 g          521 t
ORIGIN      
        1 aataaaaaca gagcaaacag gaaaactgtt aaacagaaaa ataaccacca ctcccacacc
       61 agcgaaacta ttatcatatc gaaatgcaga tttaataaaa gaaaactaca ttacagaaaa
      121 agttttatct attttcaata taaaacggga tttcgttgct gtaagaatcc aaagtaatca
      181 gtttactgat ttaaaaaaca agacaattca gggtcataaa gacactgtcg caaaagtcat
      241 agactggtat aacccacaaa aaaacgcatt tggtatcatg atgggcacac caagacgttc
      301 tgctgatata gcaaaggaag aatccagaaa cgctctaaat ttcatgataa tggaaaagga
      361 tacatttaat gaaaagatac ttaacagcaa tgctaattta caaaaaaaat acggcacaac
      421 agaaaatagt agttgggtat cggcttctgt tggttctctt ctggacaaag gagccaaagt
      481 atatccagat atatcatgct ctctgagact gggagaacca ttcattatta cacttccaga
      541 aacagtcagg ctggatgtta atatccaccc actcaaaaaa tgatcctgaa cgtttttatg
      601 caggcaaatg cgtccacatc aaggcttcaa ccttgtgctc tccccctgtc acaggataaa
      661 tttgcctgta aaaatcaaac tctccctgct cattaccaat accaatcagt tgaatgcagt
      721 tgccatctac aataaatttt tccgctgacg taaccaaaat aaaggcttcc ggatgttcat
      781 taggaaaggt gttagcaaga gtacactgaa tatccatctc caacaaaatc tcctgattat
      841 tagcgtaaac tcatcgacga aatcgttact atcccctggg cacatatgta ccattgaata
      901 ctactgttct ccagattctg ataatccatg gcgaacaata agaacactac agccgctttg
      961 ctgattagtt agccataacg gtgaactttg ctggatagtt tgagaaatat catttcgccg
     1021 gaggcttata cactgattac tcaagcttac aactgcaggt gcatcaaagc gctccggact
     1081 atcagtatca cgcatagctg taaacagaat ataatcacta ccagccatat catattccgt
     1141 ttcttttagt cttaacaagc catcctctct tgtaatatta actgtaatta aagatgtctc
     1201 atcatgatgg gcatagcgct gtgtattaag aattaaatgc tcttccagaa attttacagg
     1261 gttactacgt atttcattca ttatacaccg aagctctgaa cgatgcggca ccagaaagtg
     1321 cccggtattt tgtgaaataa tactacttac cttgattgtt ttctctccag ctcatgaaaa
     1381 ataactcgcg tattatattg ttttaccaac cacaggcccc gaatgcctgt ggtgttttta
     1441 tataaattat cggaagttaa tattgtttca tttgaaacat cttaactatc ttttataata
     1501 aagcttcccc ttgcagggtc atatttacac tcttctggtt taacaatcat tgatggggtt
     1561 attggagctc gtgtcagagg gtgaactgaa ccgtcattaa ccacgcgaga aagtgcatca
     1621 acatcaaata aagtgcatac tgctgaacta tctgaatttt tgacaaacac tccttcttcg
     1681 ggtctctcta gtgtaattgg acactgaata gactctggcg ggcactggaa atttcctgaa
     1741 ctaacaggaa acttacattg agatattttg tcctgaatac tctcttgcga ttgtgcttca
     1801 cctgagtccg
//
LOCUS       DAZKGS010000375         1809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKGS010000375 DAZKGS010000000
VERSION     DAZKGS010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(310..582)
                     /locus_tag="ACHOQY_005154"
     CDS             complement(310..582)
                     /locus_tag="ACHOQY_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681039.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            742..1275
                     /locus_tag="ACHOQY_005155"
     CDS             742..1275
                     /locus_tag="ACHOQY_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9681040.1"
                     /translation="MHEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1496..1609
                     /locus_tag="ACHOQY_005156"
     CDS             1496..1609
                     /locus_tag="ACHOQY_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681041.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1611..>1809
                     /locus_tag="ACHOQY_005157"
     CDS             1611..>1809
                     /locus_tag="ACHOQY_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFP9681042.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDHAIIYKEQRDKKASEL
                     ELANATITDMQIRQRDVAALDA"
BASE COUNT          481 a          354 c          488 g          486 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat
      421 attatgtttc agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt
      481 tttatgtgag atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc
      541 tgccatcaga agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct
      601 gcgcaaactt tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc
      661 aagggatatg tttatgagag cgaccatgag tgttggatta tatacctaac atatcaaggg
      721 attagaaatc gataaatccc catgcacgaa aaaataaaat acggcctgtc ggctgccgtt
      781 ctggcgctga ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa
      841 aaggaaggta accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt
      901 ggtgccattc tggtggatgg taagcctgtt attcctggca tgaagctgtc aaaggaaaaa
      961 tgcgaccggg ttaatgccat cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc
     1021 cgggtgccgc tgaccgaacc ccagaaagcg gggatcgcgt cattctgtcc gtacaacatt
     1081 ggccccggta agtgcttccc gtcgacgttt tataaacgaa ttaatgcagg cgatcgaaaa
     1141 ggtgcctgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
     1201 tcaaataatt gctacggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
     1261 gacatcgaca gatagcagaa tattttcctg aaaaatgacg ttggccaacg cgggtggata
     1321 acacgaaatc ctgaaaactg gtaaaaccta agtgaataaa agtaaaaacc ccgtttgttg
     1381 gcagcaagcg gggttttgtg ttttctgacc ttgagcaagg caagggagaa attatgggta
     1441 gggaggtact ttccctgtga ggaagtataa aagattcttt ctgaggttgt ccattatgaa
     1501 aggcattgaa gtggagacgc cagccagtct ggatttaaca agagcggcag cttttgccat
     1561 tcgtattgtg gccattgctg ttctggtctg ggcaatccgt tggtggtgat atgaaccgtg
     1621 ttctgtgcgt ggttatcatt gtcctgctgg tggcctgtgg tgcgcttagt ctggggctga
     1681 atcattaccg tgatcacgcc atcatctaca aagagcagcg cgataaaaaa gccagtgagc
     1741 tggagctggc gaacgcgaca attactgata tgcagatacg ccagcgtgat gtcgctgcac
     1801 ttgatgcca
//
LOCUS       DAZKGS010000376         1779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKGS010000376 DAZKGS010000000
VERSION     DAZKGS010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            160..1776
                     /gene="tir"
                     /locus_tag="ACHOQY_005158"
     CDS             160..1776
                     /gene="tir"
                     /locus_tag="ACHOQY_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP9681043.1"
                     /translation="MPIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMG
                     SRLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNS
                     AIGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRG
                     GAGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLR
                     ADPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLT
                     KEAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQ
                     KYDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVV
                     DNQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRN
                     DSLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGL
                     RLGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          498 a          392 c          451 g          438 t
ORIGIN      
        1 tctgcattat tttcttgcat caaaaatcag actgtgattt atttggtcga tgttatgttc
       61 agccgtatat cgactacgta ctgtatacca ttacattatt atgtaatagc aatataaagg
      121 attattatct atttacataa aataaaaagg agatatttta tgcctattgg taatcttggc
      181 cacaatccca atgtgagagc tttaattcca cctgcaccgc cattaccttc acaaaccgac
      241 ggtgcaggag gtgcccgtaa tcagctcatt aactcaaatg gcccgatggg gtctcgtttg
      301 ctatttacgc ctataaggaa ttctgttgct gatgctgctg attctcgtgc cagtgatatt
      361 cccggacttc ctacaaatcc actgcgcttt gctgcgtccg aggtatcttt gcatggtgcg
      421 cttgaagttc ttcatgataa aggggggctt gatactctta actctgctat tggatcttcg
      481 ttattccgtg ttgaaactcg ggatgatggc agccatgttg ctatcgggca aaaaaatggc
      541 ctcgagacca ctgttgtttt aagtgagcaa gagttttcta gcttacagtc ccttgatcct
      601 gaaggtaaaa acaaatttgt atttactgga ggccgcggtg gcgcagggca tgctatggtc
      661 acggttgctt cagatatcgc cgaagcccgt cagaggataa tagataaatt agaaccaaag
      721 gatacaaagg agacgaagga gccaggggat ccaaatagtg gcgagggaaa aatcattgaa
      781 attcatacct caacctcaac ttctagcctc cgtgcagatc ctaaactttg gttgtcattg
      841 gggactattg ctgcaggtct gatagggatg gctgcgacgg ggattgcaca ggctgttgcg
      901 ttgactccag agccggatga cccaatcact accgaccctg atgctgcagc aaacacagct
      961 gaagcagcgg caaaagatca gttaacgaaa gaagcattcc agaacccaga taaccagaaa
     1021 gttaatatcg atgagaacgg aaatgcaatt ccgtccgggg aactaaaaga tgatgttgtt
     1081 gcgcaaatag cagaacaagc taaagcggcg ggtgaacagg ccagacagga agctattgaa
     1141 agtaattctc aggcgcagca aaaatatgat gaacagcatg ctaaacgcga acaggaaatg
     1201 tctctttcat cgggggttgg ctacggtatt agtggtgcgc tgattcttgg cgggggaatt
     1261 ggtgccggtg ttactgctgc tcttcatcgg aaaaaccaac cggcagaaca aacaatcact
     1321 acacgtacgg tagtcgataa tcagcctacg aataacgcat ctgcgcaggg caatactgac
     1381 acaagtgggc cagaagagtc cccggcgagc agacgtaatt cgaatgccag cctcgcatcg
     1441 aacgggtctg acacctccag cacgggcacg gtagagaatc cgtatgctga cgttggaatg
     1501 cccagaaatg attcactggc tcgcatttca gaggaaccta tttatgatga ggtcgctgca
     1561 gatcctaatt atagcgtcat tcaacatttt tcagggaaca gcccagttac cggaaggtta
     1621 gtgggaaccc cagggcaagg tatccaaagt acttatgcgc ttctggcaag cagcggcgga
     1681 ttgcgtttag gtatgggagg attaacgggg gggggcgaga gcgcagtaag tactgccaat
     1741 gccgcaccaa cgccgggacc cgcacgtttc gtttaaata
//
LOCUS       DAZKGS010000377         1760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKGS010000377 DAZKGS010000000
VERSION     DAZKGS010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..287
                     /locus_tag="ACHOQY_005159"
     CDS             <1..287
                     /locus_tag="ACHOQY_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681044.1"
                     /translation="PKSNDRETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDW
                     KPIAQGAKKVKADVYTSSDRNEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            complement(336..1160)
                     /locus_tag="ACHOQY_005160"
     CDS             complement(336..1160)
                     /locus_tag="ACHOQY_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFP9681045.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
BASE COUNT          568 a          349 c          347 g          496 t
ORIGIN      
        1 aacctaaatc aaacgataga gaaacaaatg aatatgtgta tgacatggcg acatttgaag
       61 gaataagtta tctaggagcg agaagcccat tgacgcaaat tgcagaacaa attaaaggta
      121 taagagagga ttggaaacct attgcacaag gagctaaaaa agtaaaagca gacgtatata
      181 cttcaagcga tagaaacgaa gaacgcacgt atctgcaaga gcaacacgat ttggcaataa
      241 aaaggagaga tgagaaaaga gaaaaaagat tagagtctgg tgaataattt taaagggagt
      301 gggtaactaa cccactcgta actataaacc tgtaattaat cacttatttt tgacaacaga
      361 taattactga acgcactgca agtgactaac ataaatttag cttcagctaa ggtaggattt
      421 acatcttctt ctgttagcgc atgacgtatt ccaccctgat cacttgtata accatagagc
      481 tgactaaaag cgcctttcat tgcagagtgt atatatcctt tttcctctat agctttaaga
      541 caagccccca aggttccttt atcattgccc gtgattttcc tgcataaaga ttcaattgca
      601 gagatagact ctttaatcga gtttctgtag tctggctgct ctctatccgt cattagttgt
      661 aacgcccttt cgaaatggct acgcgatgaa tcagtgccat tatcaactgc gttctgaaca
      721 ctttcaattt cgttatcatt tgaaatagga gtaatacaac catttattat ggtataacca
      781 acgccatgct ttttaaagat ggaattgaga tgcttcgata gattaatata tgaattagtt
      841 ctctcaatga tgaactcaat taaatcatat accaaatacc atgcttcccc atatatataa
      901 tctcggatag cagtcagcaa cgtcttatca cttttgtatc cactttcata acgaggaata
      961 ttatccgcag gttgatttag ataatatatc cacacagact gcgcacattt tgttgctgta
     1021 gcagtttgac gattgttagt ccaaaggaaa agatataagc aattccacaa tgccatgcgt
     1081 gtatcagaat taagatcatt cagctggaca tgctctctaa cgtcaacatg accatacctc
     1141 acagaaaatg gctttatcat tcttcccctg cctgattgtt atgttcgttt aaattatgca
     1201 gtctaagttt gatacaatca tatattaagc aatagaatcc ataaagcccc gttggtggta
     1261 agacaacaag cggagcctct tgcgacttca tcagggttat gtcagttagt gaggcttatc
     1321 aggatgacca tgtacccaag gggagcgagg ccgaaccgct ggcagagctt gcatatggtc
     1381 tgcaagtacc gtgaccacca taaacctgtt tatcccctgc ggcgtctcgc gacggcgaca
     1441 gaatgggggc atcgggctaa aatgcgctgc actaaaagtt ttgggattgt ttagagacgg
     1501 tggctacgtc atagctttca agttgagtca gtacgtgttt gacgcttgga acgacacgcc
     1561 gctatgagtt aacatcccaa ttaccactat caaccacagc ggctttttca agggacataa
     1621 cagagcattt cttacaggca aaagagacag atttaatcgt ctaactaact gtattgtaag
     1681 gaatttagta acttggtgcg aaggccggac tcgcacacac atttaaccta ttgtaaaaat
     1741 tgaaatttaa ttataattaa
//
LOCUS       DAZKGS010000378         1753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKGS010000378 DAZKGS010000000
VERSION     DAZKGS010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..867)
                     /locus_tag="ACHOQY_005161"
     CDS             complement(<1..867)
                     /locus_tag="ACHOQY_005161"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9681046.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDA"
     gene            complement(848..>1753)
                     /locus_tag="ACHOQY_005162"
     CDS             complement(848..>1753)
                     /locus_tag="ACHOQY_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681047.1"
                     /translation="FIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          342 a          572 c          473 g          366 t
ORIGIN      
        1 cgcatcggtg ctgttgacaa gttcatcagc cagtccggca tcaatggcct cctgaccgct
       61 gtacactgca gcctcggtat ccagcacagc ctgcacggac aggccggtat atgccgacac
      121 cttctgcgca aacatccggc gggttgcatc catccgggac tgcagtgttt cccggacatc
      181 acccggtaga tggctgtagg ggttgccatc caccttatgg ctgccgctgt aaatcagcgt
      241 gatttccacg ccctgtttct ccagggcagc accgtaatta ctgtgagcca tcatgacgcc
      301 gatggagcct gtccgggcgg tctgcgtgac cagacgccgg gaggcggcgc tggcaagcag
      361 ctgacctgca ctgcagttca tgtcgttggc cagcgcccat accggtttta tgtcacgcac
      421 acgggcgatg atgtcagcac agtcaaatgc tcccgccacc attccgcccg gtgtgtccat
      481 atcgagcaga atgccgtcca ccatcgggtc gctggcagcc tgttgcagac gggcgataat
      541 gccgttgtaa ccggtcatcc ccgaatacgg ctgcagcgcc cgcgtccggc tgaccagcgt
      601 accggacacc ggcagcacgg cgatgccgtt catgacctga taactccggg cctgtcgtgg
      661 tccgtcatca tcaccggata acgccagcgc cgcgggtgcc tctccggcag tcaggctgtc
      721 gccggatact gcatccgtca ggcggctgat cccaagctgg cctgcaagcg cacaaaagaa
      781 aacccgcgca taggcgggtt caagcatcag cggctcatta aaagccatgc tggcaatatg
      841 cgggagatta cgcagctctg ctgtcactct tctcctcctc tgttgattgt cgcagcccgg
      901 attcaaatgc cgcagccgcc caggcgggcg gtttaagacc agctgcgcgg cgctccatcg
      961 tttcacggac ctgctgggca aaaatttcct gatagtcgtc accgcgtttc gcgcactctt
     1021 tctcgtaggt gctcagtccg gcttctatca gcatcaccgc ttcctgaact tctttcagac
     1081 catcgatggc catacgaccg gagcctatcc agtcgcagtt cccccaggca ctgcgggctt
     1141 cctgaaagct gaagcgcgct tttgaaggta acgtcaccac gcggcgaacg atggcctctt
     1201 ccagccagca cagaaacatc tggctcgcct gacgggatgc gacgaatttt cgccgcccca
     1261 taaagtacgc ccacgactcg ttcgcactgg cccgtgccgt ggagtagctc atctgggcgt
     1321 aattccggga aagctgctca tacgagacac ccagcccggc agcgatatac cgcagcagtg
     1381 actgctcaaa cacggagtag ccgttatccg tgtcctgagc cgtctgcagg ttcagtgagt
     1441 cccccggcat caggtgcggc acttttgcgc ctcccagacg gaccggtgct gcggagtaat
     1501 acgcggcaat ttcaccaatc cagccggtca gcctttcccg ctgctcctga ctgttcgcgc
     1561 ccagaataaa atccatcgct gactgcgtat ccagctcact ctcaatggtg gcggcataca
     1621 tcgccttcac aatggcgctc tgcagctgcg tgttctgcag cgtgtcgagc atcttcatct
     1681 gctccatcac gctgtaaaac acatttgcac cgcgggtctg cccgtcctcc acgggttcaa
     1741 agacgtgaat gaa
//
LOCUS       DAZKGS010000379         1752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKGS010000379 DAZKGS010000000
VERSION     DAZKGS010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1752
                     /locus_tag="ACHOQY_005163"
     CDS             <1..>1752
                     /locus_tag="ACHOQY_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFP9681048.1"
                     /translation="DFLINDERVFNLEGLIKKYKYLSLSEIHKTLTNSNSFADISIPL
                     LQTICPSITTIIKKTEYHGHQLTNAMTVASVVKPYDFSNLGAINSIDKSVSDIPALHT
                     IVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSKGKCLGLSLL
                     YLDTGGYGSRYQKLRHNIETASALYQTKYNDNIKLSNSDDFFLRKTQRIITMSNELGN
                     NRLKNSQLEILELKDPVLTEGILYQRRISSLLITTEYHSLALQQVSSFWRVTDPNFGH
                     CDFHSLAQALTFIKNITSNGNFSSLYGSGIIKIYFSESPNNWKYIKLPLIQNNSLSRD
                     IYLTTPEKLSATGDSLNIMGHLVPVSFIYDIGGVFNGNRISESTDIKNKIKNLKINGD
                     ILQHYINTHYLSESQTQKIKDIVDFLGIQDNTKRVKLESDIKHISEVQQPLHSIISRQ
                     KEHVKNLLSGLLNEFSNKLRQQGLSLKTNVLSVNNFKASKINSDTVEVTVTDLQGRLY
                     SVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQYTRMIKTNDNISAIDHAG
                     AVSDIKNIVDKFLGGILT"
BASE COUNT          661 a          257 c          287 g          547 t
ORIGIN      
        1 ggatttttta attaatgacg agagagtttt taatcttgaa ggcttaataa aaaaatataa
       61 atatttatca ttgagtgaaa tacataaaac tttaacaaat agtaattcat ttgcagatat
      121 ttccatacca ttattacaga ctatatgtcc cagtataaca acaataataa aaaaaacaga
      181 atatcatgga catcagttaa caaatgctat gacagttgct tcagtggtta agccatatga
      241 ctttagtaat cttggtgcca taaatagtat agataagtcc gtttctgata tacctgcatt
      301 gcatacaatt gttgaacaag cgaagtataa tttactttca tggaatgatt tttataatac
      361 tcatgcgtca atatgggata caatagcaag gcagcataaa agtacaaata tcgaatttca
      421 cccacaaagt cttctttttg atagagattc gaaaggaaag tgccttggtt tgtctctcct
      481 gtatcttgat acaggaggtt atggaagcag atatcaaaaa ctgagacaca atattgaaac
      541 tgccagcgca ttgtatcaaa caaaatataa tgacaatatt aaattatcta atagcgatga
      601 tttcttttta agaaaaacac aacgtatcat tacaatgtcc aatgagttag gcaataatag
      661 attaaaaaac tcacagttag aaatcctaga attgaaggac cctgtactaa ctgaaggtat
      721 attataccag cgtcgaatat catctctttt aataacaaca gaatatcact cactcgcgtt
      781 acaacaagta tcttcttttt ggagagtaac cgatcctaat tttgggcatt gtgacttcca
      841 ttccttagca caggctttaa cgtttataaa aaatataacg agcaatggta acttttcatc
      901 tttatatgga agtggtatta ttaaaattta ctttagtgag tctcctaata attggaagta
      961 tataaaactc cctcttattc agaataattc tttgtcaagg gatatatatt tgacaactcc
     1021 agagaaatta tccgcaactg gagattcatt aaatattatg ggacatttag tgcctgtcag
     1081 ttttatttat gacattggag gagtctttaa tggcaacaga ataagtgaaa gcacagatat
     1141 aaaaaataaa atcaagaatc tgaaaataaa tggggatatt ttacaacatt atataaatac
     1201 gcattatttg tcagagtcgc aaactcagaa gataaaagat attgtggatt ttttaggcat
     1261 tcaggacaac acaaaaagag ttaagcttga aagtgatatt aaacatataa gtgaagttca
     1321 acagcccctt cattctatta tctcacggca gaaagaacat gtaaaaaact tactatctgg
     1381 attacttaat gagttttcaa ataagttaag acagcaaggc ctttcactta aaacaaatgt
     1441 tctttcagtt aataacttca aagcatcaaa gataaatagt gatactgtgg aggttacagt
     1501 aactgatctg caaggaagac tttatagtgt tgatatagat acaagagtta ttggcttaac
     1561 ttttaaagag gggataaata gtttatctga agcactcgaa cacatgaata tagatgcaat
     1621 tatgtctgtt attggcttgg ttcaatatac acgaatgata aaaacaaatg acaatattag
     1681 cgcaatagat catgcaggag cagtatcaga tattaaaaat attgtagaca aatttttagg
     1741 cggaatttta ac
//
LOCUS       DAZKGS010000380         1743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKGS010000380 DAZKGS010000000
VERSION     DAZKGS010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..663
                     /locus_tag="ACHOQY_005164"
     CDS             <1..663
                     /locus_tag="ACHOQY_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9681049.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
     gene            666..992
                     /locus_tag="ACHOQY_005165"
     CDS             666..992
                     /locus_tag="ACHOQY_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9681050.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..1352
                     /locus_tag="ACHOQY_005166"
     CDS             1002..1352
                     /locus_tag="ACHOQY_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9681051.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            1349..>1743
                     /locus_tag="ACHOQY_005167"
     CDS             1349..>1743
                     /locus_tag="ACHOQY_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9681052.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAA"
BASE COUNT          373 a          420 c          597 g          353 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 tgaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaacgct gccggagaaa
     1081 tgacggaaga atgggtgtca tgcgggaaaa ttcatgcgga tatccgtggc aggagcagcc
     1141 gggagcggat gcagtctggt gcggaaatgg cgcaggcgga aatccgcatc tgggtgcgcg
     1201 gtcagtccgg tcgggaaatc acggcagcgt cacgacttca tgtgctgagt ggtccctggc
     1261 gtgaccggat cctgaacgtt gtcgggctgc ccgtgccgga tgcgaccggc ggacgtctgg
     1321 aaattctctg tcggctggga ggtgaaaaat gatcgaaacc ctgctggatt tttcggggct
     1381 ggaggacatc agccgcgatt tgcagcttct gagtggtgca gaaaataacc gggtgctgcg
     1441 tgaggcaacc cgtgcgggtg cgaatgtgct gaaagaagaa gtggtgtcac gggcaccggt
     1501 acgcagggga aaactgcgcc gcaatgtggt ggtcctttcc cggcgctccc gcgatggcgg
     1561 gatggaatcc ggtgtgcata tccggggtgt taatccggac accggtaaca gcgataacac
     1621 catgaaggcg gataacccgc gcaacgcttt ctactggcgg tttgtggaaa tggggaccgt
     1681 gaatatgcca ccgcacccgt ttgtgcgtcc ggcgtttgat gtgcgcagtg aacaggcagc
     1741 gca
//
LOCUS       DAZKGS010000381         1730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKGS010000381 DAZKGS010000000
VERSION     DAZKGS010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..>1730
                     /locus_tag="ACHOQY_005168"
     CDS             8..>1730
                     /locus_tag="ACHOQY_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681053.1"
                     /translation="MIPNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYP
                     ITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINI
                     PQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLGSIIHEIIPKENESAE
                     TVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALF
                     FNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPT
                     IFYQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRT
                     IGRGVSYVDIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLS
                     LMSSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNP
                     DILNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQ
                     GERAILNTSQSETCKHKIDEIYEKRFSETELNTLPNEMKNFLIFLNKTHEKDISRLSM
                     GNEVNEKI"
BASE COUNT          653 a          332 c          270 g          475 t
ORIGIN      
        1 aggaaacttg attccaaatt caacttcaaa taaaggttat gtatgcattg acatgcagtg
       61 ttcgtcaacc tctgaaccaa cagcttctac ctccagcaac cggagtatta aattagccgc
      121 ttccacaaat gtatatccga taacaagaaa cgactccgag ctcactctga acgattttct
      181 tgataatagc tcttctacgt catcattgga ctacattaat gaattgggtt ctcaactgac
      241 gttaaatgat tttcttgaca acataaagac aaatgaggtg gatagaacat gtacggatgt
      301 ggtaattaat atcccacaag agatacaaac aaatacacag gaaaatgatt tgttattatc
      361 cgataaaaat aattcaatat gcattgaaat cgatgaaaga attacaaaaa tcctaacatg
      421 caagcaaaaa tatcaactgg gcagcatcat tcatgaaatt ataccaaaag agaatgagag
      481 tgcagaaact gttctccatc ttatgagagt tctgaacgat caatatcatc aggtatataa
      541 tcagtcagga tgtttttata aagcctatat ggccatacac aataaaatcg aacagatact
      601 tccatatgcg ttcagagccg gaggcggaat cagcattcac ttgctcatac aggcattatt
      661 ttttaatggc gactataaca aatcaccttc acagtctcaa caaccatctt tatatacatc
      721 accttctcca acaataaata cagaagcatt cttaagtaat gtattatcac tagatataac
      781 ccaggtacgc atacttggtg atttactatc agcaacttta tttcatgcac caacaatatt
      841 ctatcaatat cctaaactaa tagatgaagt taagtattgt ataagtaata aaaaaataac
      901 aggttcggtt atagcacgat ttactctatg tttaacaagt acattactaa ccatgtcacc
      961 actgttaatg cttaatggag cagttaaaac aggtagcata gtaagaacta taggtagggg
     1021 agtgagttat gttgatatac cattggcctt agctatatta ggtgactcgt ggtataaagc
     1081 ttataaacat ggttcttctg ataacccaaa ttctgctcag agatttatat cgcaagaagc
     1141 ggcctttaaa accacccagc gggtattaac acaaggatta agtctgatgt cctctttatc
     1201 gggagcaatc atgcgctctc ttgagaaagg cacaccacca caaatgatgt ctttattcat
     1261 cgtaaacata ctaaatctat tatttcatca aaatccatat gaaggggcat cagcaagtgc
     1321 taatgctttg aaaagatcaa cttactccca taatccggac atactaaata ctcaggcaat
     1381 agctctttgt gttgacctcc agcatacaaa aaatataacc atgccacttt tcaaaacaaa
     1441 agacaggata tcttacgcat tcaatggaca aagaacatcc ccagaagacc aaaaacaaat
     1501 actgaaagaa gttataaact cctgtaccca aggagaaaga gccattttaa atacatcaca
     1561 atcagaaaca tgcaaacata aaatcgatga gatttatgaa aaaagattct cagaaacaga
     1621 actaaataca ttaccaaacg aaatgaaaaa tttcttgata tttttaaaca aaactcatga
     1681 aaaagatatt tcgcgtttaa gcatgggtaa cgaagttaat gaaaaaataa
//
LOCUS       DAZKGS010000382         1714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKGS010000382 DAZKGS010000000
VERSION     DAZKGS010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            272..1474
                     /locus_tag="ACHOQY_005169"
     CDS             272..1474
                     /locus_tag="ACHOQY_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681054.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          470 a          349 c          438 g          457 t
ORIGIN      
        1 gccagttgtc gaaccccggt cggggcttct catccccccg gtgtgtgaaa tatacgaaaa
       61 aaaagcccgt actttcgtac gagctcttct ttaaatatgg cggtgagggg gaccgcctga
      121 aataaatctg acttaatcca ggggtgttat ataatttcat tataaacaat gtattacact
      181 ttttgttgtc cgatattatc taatgtattc ttagtgaagc gagagactaa tggcatactg
      241 gatggcatac cagagtgacg ggaattagcg tatggcagta ttgacggata cgaaagcaag
      301 acatatcaaa cctgatgaca aaccattgcc ccatggggga attaccggac tgacccttca
      361 tccttcttca gtaaaggggc gggggaaatg ggtttttcgt tatgtaagtc cggtgacaca
      421 aaaaagacgt aatgctggat tgggaactta cccagaggtc agtattgctg aagctgcacg
      481 tactgcccgg ataatgcgag agcaacttgc tgcaggtgat gatcctctgg agattaaaaa
      541 ggctgaatct gagaaagtcg ctatcccaac atttgccgat gcagccaggc gtgtacatgc
      601 agaactgtct cctggatggg aaaatccaaa gcatgtaagg cagtggttat cgacgcttga
      661 gaattacgcg tttcctcaac tgggagcaaa aacgctggat tcgattacgg ctgcggacgt
      721 ggcagaaaca ctgcgtccag tctggttaac cttgtcagaa acggcaagcc gggttaaaca
      781 gcgcattcat gttgttatgc agtggggatg ggcgcacggt ttttgtgtag caaatcctgt
      841 tgatgtggtt gaccatttgc ttcctcagca gacaagagga cgtgatgaac accaacccgc
      901 aatgccctgg aggcagttac cgctttttgt ggcgaccagt gtgtataccg atgaacctta
      961 taatgttacc cgcgcactgt tattaatggt gatacttaca gcaactcgct cgggcgaagc
     1021 caggggaatg cgctgggctg aaattgattt tcataagcgg gtatggacta tacctgcaga
     1081 aagaatgaaa gccaggatac agcatcgtgt tcctttatcc cggcaggcta tttacattct
     1141 ggaaaatata cgtggcctgc atgatgaact ggtgttccct tcacccagaa agcagcagat
     1201 cctttccgat atggtgttga caagttttct gcgtaaaaag aaagccgtca gtgacattcc
     1261 ggggcgagtt gccacggcac atggttttcg ctcaacattc agggactggt gtagcgaaca
     1321 ggggtattcg cgggatctgg cggaaagggc gctcgctcat acgctgaaaa ataaggttga
     1381 ggcggcatat catcgtactg atctactgga gcagcgtgta ccgatgatgc aggcatgggc
     1441 ggattatgtg atgtctcaaa ttgtgaataa ataatagtga aaatgtatat tgtacgatca
     1501 tgatttgata tgtgatcgta gcagtagtaa ttaagaaacc gcttttatgt caaatgtatt
     1561 gagctgataa ttatcagatc gtcaggaaag cgtattttga ttaacagggg attacttaac
     1621 aatccccttt ttataaaaaa taatttgcct gtttaactct tcactcctgc tgagactgaa
     1681 ttattcctag tgcgtcataa atcagtcttc tgcg
//
LOCUS       DAZKGS010000383         1710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKGS010000383 DAZKGS010000000
VERSION     DAZKGS010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(35..355)
                     /locus_tag="ACHOQY_005170"
     CDS             complement(35..355)
                     /locus_tag="ACHOQY_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9681055.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            463..636
                     /locus_tag="ACHOQY_005171"
     CDS             463..636
                     /locus_tag="ACHOQY_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9681056.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            707..1630
                     /locus_tag="ACHOQY_005172"
     CDS             707..1630
                     /locus_tag="ACHOQY_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9681057.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKKTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSCRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWLRLKNKELPPSLHHY"
BASE COUNT          486 a          371 c          374 g          479 t
ORIGIN      
        1 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
       61 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      121 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      181 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      241 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      301 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      361 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatagtg gcattatggc
      421 cttcatggtg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      481 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      541 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      601 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      661 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      721 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
      781 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
      841 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
      901 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
      961 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1021 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1081 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1141 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1201 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggagca
     1261 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taatcggcaa
     1321 acaagagaag aaaaccaacg agctttctgc aaaagaagca aacagccttg tatggttatg
     1381 ggattatgct aaccgctcac aggcattatt ccgcgaactg tatccggcgc taaagcaaat
     1441 tcaatcgaac tattcctgca gatgctacga ctacggtcat gagttctcgt atgttattgg
     1501 aatggcgaga gacgttttaa tcaatcacac gcgagatatt gatattaatg agccagacgg
     1561 accaacgaat ctttctgcat ggctaagact taagaacaaa gaattacctc cttcactgca
     1621 tcactactaa cagattgcca acgaaatgac ccagcttcgg ctgggttttt tatcaggagt
     1681 tctcatgctc tatagcaata tattggcgca
//
LOCUS       DAZKGS010000384         1703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKGS010000384 DAZKGS010000000
VERSION     DAZKGS010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            281..931
                     /gene="nleG7"
                     /locus_tag="ACHOQY_005173"
     CDS             281..931
                     /gene="nleG7"
                     /locus_tag="ACHOQY_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFP9681058.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(1156..1703)
                     /locus_tag="ACHOQY_005174"
                     /pseudo
     CDS             complement(1156..1703)
                     /locus_tag="ACHOQY_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT          541 a          346 c          299 g          517 t
ORIGIN      
        1 cattaatatc ttgaatggat tacatagagt taatgtatcc agtactccct attctcttgc
       61 atataataca tgttgcaact tacatctcag cgctatgaaa aaacaccacc tctctcttta
      121 tgaaattctg gatttaccaa gcgctaattt atcatttcaa tctaccttta agtattgcat
      181 ttatctccct acgaggtcat actttaggaa gttaaatatg aatgataata tacctacagc
      241 gcgaaatcac aaacaatcga cttgtattac agaaaaaaac atgcctatat ttttaaactt
      301 cacagcaggc agtatcttac ctgagaatga gctagcatct ttacgttata ttgtgcagca
      361 aaatcaaaat gatactgtaa tcataaaaga acgttataaa atggatatcc gttatatcga
      421 atcagtcaat ggttttacag taaatcctgt atgcagtaat catttctcca tatttatggc
      481 gagacaaaac actattgctc gcaacctgga acagcagatc aacaacggac gaagttttgc
      541 acaaatatct caggatttta tgcttcaatt atcttcaaat ataggatgga aaaaaggggc
      601 cgaaaacgcc cttaaaaata aaatccattc tcattcattt gttgtaaatc ctgatgaatt
      661 ctcttgcgac acacaatttc ttaagtgccc aataacatta tgcgttccag aaaaaggggt
      721 ttttgtcaag aacgcactga actccaacat atgcactctt tatgataagt ctgcgttcat
      781 gaatctcaca agagaacatc taccccaccc tctcagcagg gaaaagatag taaaagaaat
      841 gattattgaa aggaatatgt gttattttga caccataagt cagcatttca taattatgga
      901 tgcagaccaa cagaaacagc attgtaaata aaatgtaata attacatact attagtgatt
      961 ctcatgcatc gtaagcggct cgccagaacc gtattgatat ttactgagcg ctcagatcaa
     1021 ctttccatgg caacagatcg cgtacccggt ttgccggcca gtcctggata tgctcccgcc
     1081 gacaatatta atggccagga aatgtttaaa acaatgattc tgtttcctaa ccatcaagtg
     1141 tatatgctgg ctcaattact caatatctat gggttgagtt ctacccaaag tggctctgta
     1201 gtcgcttctt cttacagtat gttcaggtgt tgcaggcaca gttgtggagc gtagatgcgt
     1261 tgttggttta ccatgctctg gtacataaaa aacgccagtg ccatggtaac ctgatataga
     1321 aacgttaatt ccgcgacgtt caaactcggc atagacgtgt tctgctaaag aactcttttg
     1381 accaaataat gccctggcca accagccgtt attcatattt gcggatttct ctatttcatc
     1441 aggagagaag tctttgtttt ttaatatgtt ggctgagtta caggatgtta atctgatatc
     1501 atcaatctca tgaagattat gttgaacaat gcggtcgaca atatctgtcg gcgataaaaa
     1561 ttcatctcca cacttaagaa gtggtagacc agcagaacca tccccagaca gataaatttt
     1621 ttgtattttc ctggtgttaa atcatctggc aggatggttt tcatctctgt cgctgtaata
     1681 cttatagctg cagcagcaac aac
//
LOCUS       DAZKGS010000385         1701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKGS010000385 DAZKGS010000000
VERSION     DAZKGS010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..67)
                     /locus_tag="ACHOQY_005175"
     CDS             complement(<1..67)
                     /locus_tag="ACHOQY_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681059.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHOQY_005176"
     CDS             complement(64..639)
                     /locus_tag="ACHOQY_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681060.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHOQY_005177"
     CDS             complement(654..1007)
                     /locus_tag="ACHOQY_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9681061.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHOQY_005178"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHOQY_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9681062.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..>1701)
                     /locus_tag="ACHOQY_005179"
     CDS             complement(1426..>1701)
                     /locus_tag="ACHOQY_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9681063.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          390 a          531 c          375 g          405 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcaccgc
     1621 tgtttcgagt tgcgaattag agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga c
//
LOCUS       DAZKGS010000386         1690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKGS010000386 DAZKGS010000000
VERSION     DAZKGS010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            909..1280
                     /gene="ler"
                     /locus_tag="ACHOQY_005180"
     CDS             909..1280
                     /gene="ler"
                     /locus_tag="ACHOQY_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFP9681064.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
     gene            1295..1513
                     /gene="escE"
                     /locus_tag="ACHOQY_005181"
     CDS             1295..1513
                     /gene="escE"
                     /locus_tag="ACHOQY_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFP9681065.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLIRINNK"
     gene            1526..>1690
                     /locus_tag="ACHOQY_005182"
     CDS             1526..>1690
                     /locus_tag="ACHOQY_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system filament chaperone"
                     /protein_id="HFP9681066.1"
                     /translation="MSQTKNRELLDKKIRSEIEVIKKIIAEFDVVKENVNALSEKAKT
                     DPQAAEKLNKL"
BASE COUNT          580 a          271 c          353 g          486 t
ORIGIN      
        1 ttcaggcaac ttctttttgt ctgtatataa gggatgatta ctaaggtggt acattgatat
       61 cagttagctt tcccttttcg ggttacttta tcaaagagtc ctctttatag aagagcgcat
      121 tgtgagtttt ccagattgac ttaaacgtgt ggcggcatca caccgatcaa cacgatctgc
      181 agcaaagggc aggtgtaacc aggcccatct tagcgctatg cacttttgag ccactgagct
      241 tgctaaccgt ttacccggct ttcactgagc taattacggc gataatctga tgcccagtaa
      301 aacggacttg atgtatgacg ggtcctcttt tgcgggatag atatgggaat atttcttaat
      361 gcgaatggta cggttatgcg ggggggacaa acatggttgc ttgttctgcc atttgttcct
      421 tgttcctttt tataccgccg ccgctcccgg tcagcactgg ggaatagggg gttgaggtat
      481 atctgctcct tatgtcaggg gaacaggtaa tagctatata gcggaacgtc ttagtgctgt
      541 agttattcca ttttgctatt tgtctaatag gactaatctt aaagaaaaca aaacgattaa
      601 ctaaatggaa aggcaattgt taatggcgtt tgctaaagag atgaagttct tctactttat
      661 tgatttttaa tgatttttaa gaaatatgat ttttttgttg acatttaatg ataatgtgtt
      721 ttacacatta tacatcggtg attaataact tttataagga ttttgtttga tgaagtagat
      781 gtgttctaat ttgatagata aaacgttatc tcacataatt tatatcattc gattaattgt
      841 tgatccttcc tgataaggat aagattgcta atagcttaat atattaaagc atgcggagat
      901 tatttattat gaatatggaa actaattcgc acacaacaag cccatacatt cagcttattg
      961 agcaaattga agtgttacaa caggaagcaa agcgactgcg agagcaggaa attcaaagtg
     1021 taattgagtc gattcaaaag cagattactt attacaatat aaccctacaa gagctgggat
     1081 atactaatgt gcctgatgat ggccttgctc gccggaactc atcgaaagga gtttattatc
     1141 gcaatgaaga agggcagacc tggtcgggag ttggccgaca gccacgctgg cttaaagaag
     1201 cactgttgaa tggaatgaag aaagaagatt ttcttgtgaa ggacaccgaa gacgaaataa
     1261 taccgctgaa aaatatttaa catgaaatca ttaaatgata acgataactg aactggaaga
     1321 cgaaataata aaaaataaag aagccgcaaa tattttcatt gaaaaaataa acgacaaaaa
     1381 gaacgaaatc catgaaaaaa tgaaacaccc cttggataaa gttacctaca atgaagcaaa
     1441 ggaactgctc attgcctgcg atgcggcaat caggatcata gagataatgc taatcagaat
     1501 taataataaa tagaggacga gtattgtgag ccaaacaaaa aatagagaac tgttagataa
     1561 aaaaataaga tcagaaattg aggtgataaa aaaaataatc gccgaatttg atgtcgtaaa
     1621 agaaaacgtt aatgctttaa gtgaaaaagc aaaaacagat cctcaggctg cagaaaaatt
     1681 aaacaaatta
//
LOCUS       DAZKGS010000387         1688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKGS010000387 DAZKGS010000000
VERSION     DAZKGS010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            86..1033
                     /locus_tag="ACHOQY_005183"
     CDS             86..1033
                     /locus_tag="ACHOQY_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9681067.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            1035..1213
                     /locus_tag="ACHOQY_005184"
                     /pseudo
     CDS             1035..1213
                     /locus_tag="ACHOQY_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          510 a          316 c          455 g          407 t
ORIGIN      
        1 gtgctgctga tgctgaagca tggcgtgatg attatgaagc agggaaaact gcggtcgaag
       61 catattgtga tgagtggggc gatgaatgag cgagattgac tatcaggcgc tgcgtgaagc
      121 agcagagaaa gcaactaaag gatgctacat cgtagggcat acatcgggca atcagcatgg
      181 gaatataaca ggagtttttg tttgtcaaaa atggaaagga gaacccggtg gcgtgattgc
      241 agaatgtcat gttaactgcc tggttgaaac agatgctcag gcttacgcaa acgctgaatt
      301 tattgctgcc tttaatccaa atgttgcgct ggcgcttctg gatgaacggg aaagaaacca
      361 gcaatacatc aaacgccgcg accaggagaa cgaggatatt gcgctaacgg tagggaagct
      421 gcgcgttgag cttgaagcag caaaatcaaa actcaacgag cagcgtgaat attacgaggg
      481 agtaatcgcg gatggaagta agcgcatagc agaactggaa aaacaatgcg ccgaatggga
      541 gcgaaaagca ttaagcaact ttgaagagtg tgctgcgatg gctgaacgta tcgaagagat
      601 gcagacaaaa tctgcaccag attcgtttgg catcatcggt gaaaatattc gaacacagga
      661 caatcgaata acgtcagatc ccatgttttg tgtgtatcaa aagcgcgaaa tcgttgttga
      721 tgctgattat gaccatgacc ggattgtctg ggttgacgaa gatggcaatg aagccaataa
      781 acgccatagt cgtcgtctcg agctacttca tgaaaacttt cgagagccac cagaaaaatg
      841 gcggcgcgtt gctgtgaaag atattgatga attcgttacc tgctgtttca ccgaacaggg
      901 ttgtaaagac tacctggcag tcaatggtca caatcttcgc ttgccattta tatatgtaaa
      961 aagcggtttc aggaacgctg aatatatcgg cataagaaac tggcttgctg gcattcgcat
     1021 caaaggagag tgatatggcg ttaacacacc gcgaactctg tcagattgcg tacaagttcc
     1081 ttaagcgcaa cgggttcaag gtttgttttc atgaccgctt tatagctgta accagtaccg
     1141 gagaacagcc agatgctatg ggattcagaa attcagcatc atgcctgata gaggcgaaat
     1201 gttctcgtgc tgacttgttg gcagatagaa aaaagcgttt tcgtaaaaat ccgtctcttg
     1261 gaatgggcga ctggcgattc tttattagtg agccgggaat tatttcaatt gaggatttac
     1321 ctcctggctg gggattactt cacgttgtta acggaagagt acggaaagta catgggtggc
     1381 ccaagggtaa ttgctgttgg ggtaatcctg acgataagcc atttactgga aataagcagg
     1441 ttgaatgcga ttacatgttg tctgcattaa ggcgcatgga gttgagaggg caccttaatg
     1501 aaatatatga cggtgtaatt gttaataaga aagaaggaaa cgcggcatga ccactattac
     1561 cagagagcag ttaatagctc acgcagagga gactattgaa gcacagagac tgtgtatacc
     1621 gggcacaatc gaccatgaca tcatccgcac atataagatg gatattgctg ttctggaaat
     1681 cgcactgg
//
LOCUS       DAZKGS010000388         1675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKGS010000388 DAZKGS010000000
VERSION     DAZKGS010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..330
                     /locus_tag="ACHOQY_005185"
     CDS             217..330
                     /locus_tag="ACHOQY_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP9681068.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            399..632
                     /gene="ydfK"
                     /locus_tag="ACHOQY_005186"
     CDS             399..632
                     /gene="ydfK"
                     /locus_tag="ACHOQY_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP9681069.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            949..1539
                     /locus_tag="ACHOQY_005187"
     CDS             949..1539
                     /locus_tag="ACHOQY_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9681070.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          565 a          347 c          302 g          461 t
ORIGIN      
        1 ctatgtatac ttttaaaccc aatccgtgta gagtctctgc ataagatagt ttgcagttgc
       61 cgcttcagct tgcgccataa accgcctgat ttttgctgcc acctgttagc atttctgtat
      121 acctgaaacg acaatgttta tctacgaact ttaagaacac ccaagataaa aattgtcaac
      181 tatatcatat ataacacatt actaattcga ggctatatga acagcatact gataatcact
      241 tctctcctta tcatattcag catttttagt catgccctaa taaaattagg gattggcata
      301 tccaataacc cagacaaaac cgatgtataa gtcaacatat cctgaatcag acatacaata
      361 tcgcaatgaa aatcaataat attttaagga atatcttcat gaaatcaaaa gacaccctaa
      421 agtggttccc tgcgcagctt cctgaagtaa gaattatcct aggggatgct gtagtggaag
      481 tagcaaaaca gggaagacct atcaatacca gaacattgct tgattacatt gaaggaaaca
      541 taaagaaaaa atcatggctg gataacaaag aattattaca aacagcgata tcagttctta
      601 aagacaacca aaatttaaat ggtaaaatgt aatataataa acttactttt ttatcatttt
      661 tccactttaa caacattttg ctccactttt ccacgaccaa acaacttgaa atctggttaa
      721 aataacacgc aacactattc ttcttccttg agtccgcccg gaactcgaaa aacaaaccga
      781 gttaaagcca tttttcacaa aatcgatttt gggtctcacc aaaattacgg ggttgcatac
      841 gcattcgttt attttcgaac gtgtacatac aaatatgcac aaaaataatc aaaattattt
      901 tctgagatgc attatgatat gaacaccaat ttcgtataga gtctcactat gtctcgaatt
      961 tttgcttact gtcggatatc aacgctggat cagaccatcg aaaatcaacg ccgggaaatc
     1021 gaaagtgcag gttttaaaat caaacctcag caaataatcg aagaacacat tagcggctca
     1081 gcagcaacca gtgagcgtcc tggttttaac cggttgcttg ctcgcctgaa atgtggtgat
     1141 caattgattg tgacaaaact ggatcgcctt ggttgtaatg caatggatat caggaaaaca
     1201 gtggaacaac tgaccgaaac aggtatcaga gtgcattgct tagcattggg gggcattgac
     1261 ctgaccagtc caacaggaaa aatgatgatg cacgtaattt cagcagtcgc tgaatttgaa
     1321 cgagaccttt tacttgaacg cactcattcc gggatagtaa gagcccgcgg cgcagggaaa
     1381 cgttttggtc gaccacctgt gttaaatgaa gaacagaaac aggtggtatt cgaacgaatt
     1441 aagtcaggtg taagtataag tgccattgcc cgggaattca aaacctcgcg gcaaaccatt
     1501 ttaagagcca aagcaaaact tcagacacct gacatataaa aaataatctc ggtgtgagat
     1561 gctttacgtc ttccaagccc ccttccttgc cgtaaatgga aagatacatc taattataga
     1621 atttatatgt tttaccctac ggcagtgctg gccattcaat atcctgtgca gttga
//
LOCUS       DAZKGS010000389         1675 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKGS010000389 DAZKGS010000000
VERSION     DAZKGS010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..165
                     /gene="kil"
                     /locus_tag="ACHOQY_005188"
     CDS             <1..165
                     /gene="kil"
                     /locus_tag="ACHOQY_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9681071.1"
                     /translation="DYVLHEGRTYIASANNIKKRKLYIRNLTTKTCISDCMIKVFLGR
                     DGLPVKAESW"
     gene            165..335
                     /locus_tag="ACHOQY_005189"
     CDS             165..335
                     /locus_tag="ACHOQY_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFP9681072.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            409..684
                     /locus_tag="ACHOQY_005190"
     CDS             409..684
                     /locus_tag="ACHOQY_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681073.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            783..>1675
                     /locus_tag="ACHOQY_005191"
     CDS             783..>1675
                     /locus_tag="ACHOQY_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP9681074.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVD"
BASE COUNT          532 a          376 c          379 g          388 t
ORIGIN      
        1 gattatgttc ttcatgaagg ccggacatat atcgcctcgg caaacaatat taaaaagcga
       61 aaactttata ttcgtaacct gactacaaaa acatgcattt ctgactgcat gattaaagtc
      121 ttcctcggtc gtgatggttt acctgtaaag gcggagtcat ggtgatgact aagaaaataa
      181 aatgtgctta ccacctttgc aataaaggaa ttgaagaaag caaaagcatt aaaagaccac
      241 ttcatttcat gcgtggagtt atcccaacga cggaaatgaa aaaatattgt agtgaaaatt
      301 gtgccgaaaa agaccagatg gcacacgaac tttaattaac tgactatgcg aaactgaatt
      361 tatgccagca atggcaggga ttcgctcaac cttaattaag gagaaaaaat gattaccaat
      421 tatgaagtca ctgttgtaac taccgatgac attgttcacg aggttaatct ggaaggaaag
      481 cgtattggct acgtgattaa aacagaaaat aaagaaaccc cattcactgt ggttgatatt
      541 gacggtccat caggcaacgt aaaaacactt gatgaaggtg tcacaaaaat gagtctggtt
      601 cacatcggaa agaatctgcc cgcagaaaaa aaagccggat ttctggcaac tctgattgca
      661 atgaaattaa aaggtgaaat ctgaaaaaag aaagcctgca caacgtgcag gcctgagtga
      721 agaacctggg acatttattc atcactcgca gtaattttaa tctgagttga ggttaaaaaa
      781 caatgagcac aaaaccactc ttccttttac ggaaagcgaa aaaatcatcc ggtgaacctg
      841 acgtcgtcct gtgggcaagc gacgattttg aatcaacctg tgccactctg gactacctga
      901 tcgttaagtc aggtaaaaaa ctgagcaact attttaaagc tgttgccaca aattttcctg
      961 tcgttaatga ccttccccct gaaggtgaga tcgattttac ctggagtgaa cgctatcaac
     1021 tcagcaaaga ctccatgacc tgggaactaa aaccgggagc agcgccagac gacgttcacc
     1081 atcaggagga tgcaccggaa accgaagaac cgacgggagg ccaggaagaa aacgcgcagg
     1141 cagacgccca cgaggattgc caggattgcg aagtctctgt agccactttg cggttcacac
     1201 agcgtcttct gcacattttt acgtatgcag ccggggatcg aaaatacctg catcatgcca
     1261 cccgtgaaca acgcaaacac attactgctc ttgagatgga tcaggaaaac agctatgtcc
     1321 agaatctgct gttggccata cgcagcatgg cagaaccgac aactctggat aatgccgccc
     1381 tgctccgcct gactgatgca attaaggcag tgttctctat cacgaaaaaa catcagccct
     1441 atgaatttaa gaatttcatt tcagcctggc tggataccga acacattgat cgcggtcttc
     1501 tgacaaaaga atggcgaaaa gggaatcgtg tttcacgcat cactcgcacg gcttccggtg
     1561 ctaatgctgg cggcgggaac ctcaccgatc gcggcgaagg tttcgtccac gatctgacat
     1621 cactggcgcg cgatgtagcc actggcgtac tggcccgttc aatggacgtg gacat
//
LOCUS       DAZKGS010000390         1663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKGS010000390 DAZKGS010000000
VERSION     DAZKGS010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..513)
                     /gene="ivy"
                     /locus_tag="ACHOQY_005192"
     CDS             complement(67..513)
                     /gene="ivy"
                     /locus_tag="ACHOQY_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9681075.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            694..1461
                     /gene="yafV"
                     /locus_tag="ACHOQY_005193"
     CDS             694..1461
                     /gene="yafV"
                     /locus_tag="ACHOQY_005193"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9681076.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(1582..>1663)
                     /locus_tag="ACHOQY_005194"
     CDS             complement(1582..>1663)
                     /locus_tag="ACHOQY_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681077.1"
                     /translation="CYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT          391 a          391 c          437 g          444 t
ORIGIN      
        1 gtgcggaaag ttgaagccgc gtaattgaat aacggagccg aaaggctccg tttctttatc
       61 cgctaattat ttaaaattaa agccatccgg atggttttcc aggctgccgg tcaacgccgc
      121 gaacagcacc gttttaccat caatcgaaag cgcatcgttc acattcagcc aggtgagttt
      181 ctcttgcgac gttttctcat caatagtcga gaacagcccc gtcatctgat tagatttctc
      241 ggaccacatc acagcgatac gttgcgagcc acagtcatgc ggtttgcacg cgctcatcac
      301 ctgatacgtc tcatctccca acgttacggt ttgtgcggga gtataagtac cgcctttcat
      361 cacccaggca ggcagcttat gcccttgtac catctgatta aatgcagctt tggtggtttc
      421 gccctttgca aggctgctaa tggttaaatc atcctgcgcc attgcactgg tggcgatgac
      481 cagagcggcg actgtcgtta ttgccttaaa catcattcct cccgagctta tcctgcccat
      541 gttattaacc ttctaaagcc tggcgataac acgataattt gcaaatcaga tatttccaat
      601 atcagcattt gttatcaatt tattctgcgc taaggcaact gcctgataag gtgttaaagt
      661 tataaaaccc ttcgcataaa ggagttgtac accgtgcctg gtttgaagat tacgcttttg
      721 cagcaaccac tggtgtggat ggatggccct gccaacctgc gtcattttga tcgtcaactg
      781 gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga tgtttaccag cggctttgcc
      841 atggaagcgg cagcttcgtc gctagcacaa gatgacgtag tgaactggat gacagctaag
      901 gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat tacaaacgga gtctggttcg
      961 gttaaccgct ttttgctggt tgagccgggc ggcacggtac atttttatga taagcgtcat
     1021 ctgttccgca tggcagatga gcatctacat tataaagcgg gcaatgcgcg agtgattgtg
     1081 gaatggcgcg gctggcgtat tttgccgctg gtgtgctacg acttacgttt tcctgtgtgg
     1141 tcgcgcaatc tcaacgatta tgacctcgcc ctgtacgtcg ccaactggcc tgctccgcgc
     1201 tctctgcact ggcaggcatt gctgacggcc cgcgcgattg agaatcaggc gtatgtggcg
     1261 ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt atcgcggtga cagccgggtg
     1321 attaatccac aaggcgagat tatcgctact gccgacgcgc atcaggcaac gcgcattgat
     1381 gccgagctgt cgatggcggt gttaagggaa tatcgcgata agtttccggc atggcgggat
     1441 gcggatgagt ttaggttgta gtgagtgggg gctgtattta gggcggggaa ttcagaaagg
     1501 gatgtttagt gtgctgagcg agagttaaat aataaaaaac tgggaagatt taataatccc
     1561 cccgcagggc aagagcatga attaaatttg tgttaaattt ttcattttat cagttcgaaa
     1621 cccaagctct gagcaggatg caagcacttc ttctatataa cat
//
LOCUS       DAZKGS010000391         1659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKGS010000391 DAZKGS010000000
VERSION     DAZKGS010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..656)
                     /locus_tag="ACHOQY_005195"
     CDS             complement(<1..656)
                     /locus_tag="ACHOQY_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9681078.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     G"
     gene            complement(656..997)
                     /locus_tag="ACHOQY_005196"
     CDS             complement(656..997)
                     /locus_tag="ACHOQY_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681079.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(990..>1659)
                     /locus_tag="ACHOQY_005197"
     CDS             complement(990..>1659)
                     /locus_tag="ACHOQY_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9681080.1"
                     /translation="QRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
BASE COUNT          333 a          596 c          375 g          355 t
ORIGIN      
        1 ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc cttacggata
       61 tccgtggtgg ggttgtcgaa ctcatccgcc accgcaggac cgttataccc gcattcatct
      121 ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgcccca
      181 tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag ctgcgacatc
      241 tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg attgcccgca
      301 acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac cgtggccccc
      361 accagactct gcaggtcctc cgccatcccg gtgacaaggc caaacagatt ggacaccgtc
      421 agcgacgggc gggcactgct gccccggccg ttcatctcaa agccgctgcc gtcaatcggg
      481 tatgcctcat acttacgccc ctgccaggtg accggctccc ctttttcatt cagctcatta
      541 cagaaaaaat accgctcacc accctgtacc gtcaggtcga tttcccagag taccacccgc
      601 ggtgactgct ctgacttaac cgactcgttc agactttctt cgcgaatatc ctgcatcagt
      661 tcaccacctg ctcaatcgta caactgaaat cactgtacct ggcgttatct gtgacgctcc
      721 actcccggca caccaccctc accgtccggt tatgtttcgg cggtcgccac aaaaaggcac
      781 ggtaaccacc atgccacgat aaaaattcat ccagccagcg ccgggttggt tcatccgtca
      841 cccggaacac cgcctgaaac gtcttcagtc tggcattaag tcccgtcggt cggcgctgtt
      901 cataaccgtc accaaaccga accctcacca ccgacggttt ctcactcacc tgcatccctt
      961 cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc gtcacgtcgc
     1021 atggacagca tcaccgcctg cacccgctgg tcaatcagct gcacaagact gcctgccgcc
     1081 tccgccccta tctgtccgtt agccccgtca ttctgaatgg cgatgtggta gaccggggaa
     1141 tacaccagac cggcactgcc gttcatactg cccaccgcgc gtacgcccag cgagccatcc
     1201 gccgcccggg tcaggggcat aatggcttca ggtccggctt cccccatcag cccggcccct
     1261 tttgcaaacg caaagtacgt gggcgtgtcc acaatgctgt tgctgtacgc actcaggttt
     1321 gccgaggtat acacgccgcc ttttgcattg gccaccgcac cgcccagcca gtcaccaatg
     1381 ctgccaataa atcctcccgc accggacata ccgtttgccg ccgtcttaat tccgttgaca
     1441 atcgcggcat tcataagaac ttttgagatt tcctgcagta cggatgaggc ccagttgcgc
     1501 cattccactt tgtttccgtt cagcatctcc gtgatgttat tcaccatccc tgagataccc
     1561 tccgtcgcaa gctgtgctgc ctgtgaagcg taatcggatg cattgtccac ccagttactg
     1621 agcccctcct gcaagccttt ctgccagtcc gcacgctgc
//
LOCUS       DAZKGS010000392         1658 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKGS010000392 DAZKGS010000000
VERSION     DAZKGS010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1025)
                     /gene="tssI"
                     /locus_tag="ACHOQY_005198"
     CDS             complement(<1..1025)
                     /gene="tssI"
                     /locus_tag="ACHOQY_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP9681081.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTAL"
BASE COUNT          416 a          438 c          403 g          401 t
ORIGIN      
        1 tccagcgccg tccccgctcc ctgccgtcct ggcaccgcct gtggctgttc gccgtgcagt
       61 tcacttgcca ccacctgcca ttcccggttc aggttcgcct gcggatgccc cgtcagcaca
      121 attcgtcgtc ccggccatat ctccggcgag cgactcattc cccgcgcggt ttctgcattg
      181 tttcgccagc cgtccatctg ccagcgggca aagttctgcc cgtgggcccc cttgaaacgt
      241 ccggggtagt catacacttc atactgtgtg cgctggtaat cctggtgctg gccttcctga
      301 tcaaaacgtc cggcccagcc ggggcgttta aaggtgtagt ctttggtcac cacggaagaa
      361 gggcggattt gtgcgctgta gcggaactgg ctgatgcaga gggtgctcac ctcggtacgg
      421 gtgttcgggt tccatgggat ttcaaaagat tcgggcagat ggcggactgt gtcgcacagc
      481 accaggctct ggtcggtact tttgtaagca tgctcctcat aaaagaagat gccttcctcc
      541 gccgccatcc ggcacaggaa atcgtaatca gtctcaccgt actggacaca aaactcacgg
      601 gaaggatgcg gctcgctgaa cagcggactc cattcggtca ccccgttttc ctgcaacatc
      661 gtgccgagga tgcttttgat gtcctcgttc tggaagatac ggaagttctg gcgcagaccg
      721 gcacgccaca gcggcgggtg caccttcatg ctgtacagca tctggttttt gtcgttctcc
      781 ccgagttcaa accaggtcac cacgcctttc acccggcgct gtacttcatc gccctgccat
      841 atcgtcaggt aggccatttt gtccagcacc tgcgcaaatt caagggagag aaactgctgg
      901 ctgaccaggg agagatcgag ggaaaagagc gaagagagtg actggttcag atgaaaggaa
      961 accaccgcaa aagcatccgg cggcaggccg tccacttcca gtgtgaaacg taatccggtt
     1021 gacatagcaa cctccgtgaa aatgaataat aatccgcaca tattgcatgt acaacatgtc
     1081 gggcggatga gataagcgag tcgacatctg gcgttaccgg ggaatatcag aaaaatgtca
     1141 tggttatgtg atgatttagt ctgggatttt acttcagaga aacgggtgtc tgtttagtca
     1201 acaaaataat cacaaacgca acggatgggt tctcgacgtt attcacgaca ggctcgtaca
     1261 ttacagcgag taataaaaaa cctgatatgc aaagtcaaac caatattcat aatgtctttt
     1321 tattcacaac aataaataat tcaataacaa tcattgaaat gaatgatggt ttgtataagg
     1381 aatatacgct accgccccac cctgataaca tgatctgcac attgccatct taacgtcgtt
     1441 aaacactagc ttaatagtgg atatttcaaa ttaggaattt attaaattca cctgatgaat
     1501 ataataagca aatagcatag aaaataatga caactccccc gaggggacca tttccaaagc
     1561 actgcaccac cacctggtgc cggatgatct tgcttcagtg gtaagagtac ctgatgcatt
     1621 gttcccaaaa tggtgttttc ccctgcccct ccaataat
//
LOCUS       DAZKGS010000393         1649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKGS010000393 DAZKGS010000000
VERSION     DAZKGS010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..1641
                     /locus_tag="ACHOQY_005199"
     CDS             103..1641
                     /locus_tag="ACHOQY_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFP9681082.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          372 a          474 c          499 g          304 t
ORIGIN      
        1 ggtatcgact ggcggcagcc taaaagactg cttacgtccc tgactatgtt gtaagcctct
       61 ttatcctggt cgacgctgaa tgagcctggt aatatacccg gtatgagcag ctcacttcct
      121 gacgatatca atgcactgaa acgtctcctt gccgaacagg aggcgctgaa ccgtgccctg
      181 ctggaaaagc tgaacgagcg tgaacgcgaa atagaccatc tgcaggcaca gctggataag
      241 ctgcgccgga tgaacttcgg cagccgctcc gaaaaagtct cccgtcgtat cgcacagatg
      301 gaagctgacc tgaaggcact tcagaaagaa agtgataccc ttaccggtcg ggttgacgac
      361 ccggccgtgc agcgcccgct gcgtcaaacc cgcacccgca aaccgttccc cgaatcactc
      421 ccccgcgatg aaaaacggct gctgccggca gcatcatgct gcccggaatg tggaggctca
      481 ctgagctatc tgggtgagga tgccgccgaa cagctggagt tgatgcgcag cgccttccgg
      541 gttatccgga ctgtacgtga aaagcatgcc tgtactcagt gcgatgccat cgtgcaggcc
      601 cccgcgcctt cacggcccat cgagcggggt atcgcaggac cggggctgct ggcccgcgtg
      661 ctgatctcaa agtatgcaga gcacaccccg ctgtaccgcc agtctgaaat gtacggccgc
      721 cagggcgtgg agctgagtcg ttcactgctg tcgggctggg tggatgcatg ctgccggcta
      781 ctgtcaccgc tggaagaagc gcttcaggac tatgtgctga ctgacggtaa gctccatgct
      841 gatgacacgc ctgtcccggt gctgttgcca ggcaataaga aaacgaagac cgggcggtta
      901 tggacctacg ttcgtgacga ccgtaacgcc gggtcaacgc tggcgccggc ggtgttgttc
      961 gcttacagcc cggacagaaa aggcatccat ccgcagaccc atcttgcggg gttcagtggt
     1021 gtactgcagg cggatgcata cgccgggttc aacgagctgt accgggatgg ccggataacg
     1081 gaagccgcct gttgggctca cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg
     1141 tcagccctga cggaggaagc gctgaaacgg atcggcgaac tgtacgccat cgaggcagag
     1201 ataaggggaa tgacggcgga gcagcgcctt gccgaacgtc agttgaaaac gaaaccgctg
     1261 ctgaaatccc tggaaagctg gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa
     1321 ctggcgaaag cgttcgcata cgccctgaac cagtggccgg cgctgacgta ctatgcagat
     1381 gatggctggg ctgaggcgga caataacatc gctgaaaatg cgttgcggat ggtcagtctg
     1441 ggccgcaaaa actacctgtt cttcggttcg gatcatggag gagagcgggg agcgctgctg
     1501 tacagcctga tcgggacgtg caaactgaac ggagtggagc cagaaagcta cctccgctat
     1561 gtccttgacg tcatagccga ctggccgata aaccgggtcg gcgaactgct cccctggcgc
     1621 gtagcactgc cgactgaata acacatccc
//
LOCUS       DAZKGS010000394         1630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKGS010000394 DAZKGS010000000
VERSION     DAZKGS010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1230
                     /locus_tag="ACHOQY_005200"
     CDS             <1..1230
                     /locus_tag="ACHOQY_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9681083.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..>1630
                     /gene="ybfC"
                     /locus_tag="ACHOQY_005201"
     CDS             1227..>1630
                     /gene="ybfC"
                     /locus_tag="ACHOQY_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP9681084.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSS"
BASE COUNT          467 a          374 c          439 g          350 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga
//
LOCUS       DAZKGS010000395         1629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKGS010000395 DAZKGS010000000
VERSION     DAZKGS010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1629)
                     /locus_tag="ACHOQY_005202"
     CDS             complement(<1..>1629)
                     /locus_tag="ACHOQY_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9681085.1"
                     /translation="DGVKVKVSRVPEGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGT
                     YAITAVQHVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARW
                     DTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWG
                     QQGDAASVSFRIAAPAAPSRIELTPGYFQITATPHLAIYDPTVQFEFWFSEKRITDIR
                     QVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGKSAFVEAVGQPSDDASGYL
                     DFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAI
                     QQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIA
                     MVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNAD
                     ISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGT
                     ITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          325 a          501 c          408 g          395 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcggctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tcggtaatcc gcttttccga gaaccagaac tcaaactgta
     1021 ccgtcgggtc ataaatggca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatcct cgacggtgct gccggtgcgg caatccggaa cgataccgac gccgcatcgc
     1141 cctgctgccc ccacgcattt accgcccgga ctgtcagcct gtagttcccc agcgccagtt
     1201 gcgtgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 cgtccgctgt tacggtcaga cggagcagga agctcacgcc cttcaccacc ttcggtgtgt
     1321 cccatcgcgc cagcacctga tattccccgc tgtctgcagt gacttctgcg gtcaggtgct
     1381 gcaccgctgg cggcgtgaca ccattcaccg tgccactctg ttcgccgtca aagtgcgccc
     1441 cgttatccac gatggcctct ttttccggca catgctgcac ggcggtgatg gcatacgtgc
     1501 cgtcgtcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcagct
     1561 tcagccccca tacgctgtat tcagcaacac cctcaggaac acggctcact tttaccttca
     1621 cgccgtcgg
//
LOCUS       DAZKGS010000396         1628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKGS010000396 DAZKGS010000000
VERSION     DAZKGS010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1238)
                     /locus_tag="ACHOQY_005203"
     CDS             complement(<1..1238)
                     /locus_tag="ACHOQY_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9681086.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNT"
     gene            complement(1277..1552)
                     /gene="yddL"
                     /locus_tag="ACHOQY_005204"
                     /pseudo
     CDS             complement(1277..1552)
                     /gene="yddL"
                     /locus_tag="ACHOQY_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          485 a          248 c          307 g          588 t
ORIGIN      
        1 gtattgttta tttcatctaa ggtcatgttt ttaactttta tattcctctg gtttacaact
       61 tcctctgtct tgtaagtgtg tatattatat ggggtatatg cttttttaat aggatagtga
      121 aacaattcgt tatccacatc tattccgtca gagtgaactt caaatgcacc aggcgaaaga
      181 attgccaggc cagtattaag cttaagtgct ggggaaaaca aattaaataa catgagctgc
      241 atagtagggc aattgttctt cccagaaaca tttaaaaaat ttaagttttt gctctgtttt
      301 attatgggga ataaagttcc aatatcggat gtattattag tatcaatgtg ggttatttta
      361 ttattgtcca gtcttaaact tttgatagat ggaagataag tcaggaaatc aagatttata
      421 tatttcaact ggttgttagc agcgtttaat gtctgaacat tgcagaagtt gctatagaat
      481 attttaattt cagaaagcaa attgttgtta ataagtaacg tattcaagtt gggaaataag
      541 tcattgtgta aacttgttag tttattatgg gatagatcca gcagtaagag ttcattttta
      601 ttacctgcaa caatatcagt taattgatta tggctgagat tcaggtattg cagccattcg
      661 caagattcta attgcacaaa ctctagttga ttatgtgccg cactaaaata agtaactgat
      721 gaaagcctat ctatatcaat atttctgaga gcattattat tcatagatat atgtgtaata
      781 cttacatttc tgccttgagg aaaatttatt gactcaagag cattactatt cagacttagc
      841 tttacgagtc tatcgtagtt ggttgcatca aaatgtgtga gttggttatt atcaagattg
      901 atagaggtga agttttgcag ccctggtggt tgagtggtta aattaagtcc tgataaatca
      961 atggtcactt cattagtgtc atcaattata tttcttactg cagtccagta ctgcatttgt
     1021 gcaagtggat cattgttgat gacgttaact aatacatgcc aaagatgttt tgatgatatt
     1081 agttgatcaa tgtcattggt tggcagtaaa ttgttattgc ttaagtttaa atattccagt
     1141 tttgtagtgt ttttggcgtt taaatgtgca atatggttgt cgtttaaagt gattgttttc
     1201 atcctgggat gattgtgtaa tataaggtca gtgatcatat tgtgatgggc acttaactca
     1261 atcagagatt cttgccgcgc gagccgttgg cttcatcgtt atagccttta aaatcatatt
     1321 cccagtgacc aaaaccaatc atttgatcat tgatttgcgt ttctccttta aagccgagac
     1381 gggcataagt tttatcacca tcgtcacgct tatcatcagt aaaataacgt agagcggtaa
     1441 ccttgccata aaggtcgagt ttattaccat ccttgttata gacttcggca gcgtgtgcta
     1501 cgttcgcagc taacaaacca gttacaacca ccgcaactat ttttaatttc atttaacaga
     1561 atccttttaa ttatcgttaa acgtattttc taaacgaatt ttaaacggcg tcatttataa
     1621 atgacata
//
LOCUS       DAZKGS010000397         1612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKGS010000397 DAZKGS010000000
VERSION     DAZKGS010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1612
                     /locus_tag="ACHOQY_005205"
     CDS             <1..>1612
                     /locus_tag="ACHOQY_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9681087.1"
                     /translation="LTDNIIKLSNKIGQRVYTVVSEMSIDAWITKEPQPFVYRRSGAY
                     QKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITN
                     KASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCN
                     QLLKSLYYDVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDNTL
                     ITFSNEEIIKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRTFA
                     TALTNIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLN
                     LVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQKL
                     SQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICPYA
                     FMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIISGE
                     LYFEAHQGCFTSESATKAHNRIMENKLHD"
BASE COUNT          499 a          320 c          330 g          463 t
ORIGIN      
        1 ccttactgat aatattatca aactgtcgaa taaaataggt cagcgtgttt atactgttgt
       61 ctctgaaatg tcgattgatg catggataac aaaagaaccg cagccttttg tttatcgccg
      121 tagcggcgct taccagaaac tcaccattgg ctcagactgg ggtcaactgt ttgactgtgc
      181 ctggatgcga gtttatggta aacgtccagc agataaattt agacacaaac tcgtcgctct
      241 cgttgatatc ggtggcgaag gtcttattgt ggataagaat ggttcaccca tctgcggcat
      301 cacgaataaa gcatcatctt atggcgtacc gccagataaa ccaggtaaat gggtggttga
      361 cctttctctc gttagtgaaa atgacgaggt cgagttttgg attgatgctg cgtgcaacga
      421 tttatttggc tatgtcacta atggagggat tattactgat gttcatatcg cgacatgtaa
      481 tcaactactg aaatcccttt attatgatgt tgaagtgctt ttcgactgga ttaatgatgg
      541 tcagaagttt gaatccatac atcctaaagg tattatccca gaaaaaatta tcaccaaaag
      601 aagcgagcgt actgacgaaa tcataagaat tctcgaatat attgataata cgttaattac
      661 ctttagcaat gaagaaataa ttaaatgtca aattgcaatc caatccataa ttagtaaaaa
      721 caataatcct tcagagttca ggattatggc tactggtcat gcccatttag atatagcgtg
      781 gatgtggcct ttaagagaag gacgcagaaa agcaataaga acatttgcca ccgccctgac
      841 aaacattgaa aaatatcctg attacatttt tggcgctagt cagtaccaac tttttcactg
      901 gataaaaaaa gattatccct atttcttcga aaagttaaaa aaacaaatcg ccgcaggccg
      961 ctttgagtta caaggttgct tttgggtcga atgcgattta aatctggtaa gtggtgaatc
     1021 gctgataagg caaatcatgc atgggacacg attcacactg cagaattttt caaagaagat
     1081 aaattatgtc tggcaaccgg atgtctttgg attccccgcc acgcttccac aaattctcaa
     1141 aaaatcaggc attaactata ttgcatcgca aaagctctca caaaacaaga ttaataaatt
     1201 taataattac cttttccgct ggcaaggcct ggatggctca gaaattctga tgcataactt
     1261 tcctgaagat acgtatgata gccgagcccg cgctcgctct ttagaatata ttgaacaaaa
     1321 ctataatgaa aaagaaatct gtccctatgc gtttatggtg tatggtgtcg gtgatggcgg
     1381 cgctggcccc ggtgaggaac atatagaacg attaacacgc atacgcaata ttgatggctt
     1441 acctcatgtt gatttcagcc gtgttgataa gttcttcact cacgccgacg cctttcgcga
     1501 atccttgccc attatttcgg gtgaacttta cttcgaggct catcaagggt gctttacatc
     1561 agagtcagca actaaagccc ataatcgaat tatggaaaat aaactccatg at
//
LOCUS       DAZKGS010000398         1588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKGS010000398 DAZKGS010000000
VERSION     DAZKGS010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..677)
                     /locus_tag="ACHOQY_005206"
     CDS             complement(<1..677)
                     /locus_tag="ACHOQY_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9681088.1"
                     /translation="MERLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFS"
     gene            685..1110
                     /gene="tnpA"
                     /locus_tag="ACHOQY_005207"
                     /pseudo
     CDS             685..1110
                     /gene="tnpA"
                     /locus_tag="ACHOQY_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1174..1467)
                     /locus_tag="ACHOQY_005208"
                     /pseudo
     CDS             complement(1174..1467)
                     /locus_tag="ACHOQY_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1398..>1588)
                     /locus_tag="ACHOQY_005209"
     CDS             complement(1398..>1588)
                     /locus_tag="ACHOQY_005209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681089.1"
                     /translation="NDAPGEKMTSTPSTIHSCCAVCNYSTLLVSVRLLIIWLTSHKKP
                     LRKLLILHGVIILLTLGF"
BASE COUNT          417 a          379 c          356 g          436 t
ORIGIN      
        1 ttgctgaatt tgaccctgcg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
       61 ttctgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
      121 acgccagcat ccagcccgac cattgatgct gaagggtgaa ccggagtgga tacttcactt
      181 tctgtctgaa tactgatgta ccacttaccg caggactggc tgacagtgac atttttcaca
      241 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
      301 cggctgtttt cctgatcgag cttaacgccc tgcgggtagc ggaatgcatc attctgtccc
      361 cgctttttga atcggggaaa agcagcccga ttctggaaga agtttttgta agcccgctca
      421 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
      481 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
      541 ccggcctcat gattctcatt ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg
      601 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
      661 gcctgtagtc gttccacagg tcaaattata ttttggtctg tgaaaaaaga aaccgatatc
      721 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
      781 cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt ttcaaatgta
      841 tgtgctgatt ttgaagctga actggttgaa atggatggcg aaccagatca cgtccatttg
      901 ttaattagct atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcgta
      961 tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
     1021 gttttgtgga gtcctggcta ttttgccaat agctgcggag gtgcgccaat atccgtcatc
     1081 cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
     1141 cgccctgaag gacggggttt tacggcgcac cagataaacc aggccaagcg tatgctggtt
     1201 gatcaaataa tatcccccaa gcgcagcaat attaatattc acctgcaatg ggtcgatacc
     1261 tggcttaaaa atattgtatt tcttcccttc atccaataag gattccatta actgcaagtg
     1321 cgcatggtta atttctaaga gacgctgtga ttttgcataa tgcacacctt tactttgatt
     1381 ttcactatgc acaattttta aaaaccaagg gttagcaata taataacccc atgtaaaatc
     1441 aataattttc tcaatggctt tttgtggctg gttaaccaga tgattaacaa acgcaccgat
     1501 accaacagcg tagaataatt acataccgca caacaagaat gtatggtgga aggcgtggaa
     1561 gtcattttct ccccgggggc atcgttat
//
LOCUS       DAZKGS010000399         1584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKGS010000399 DAZKGS010000000
VERSION     DAZKGS010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..350)
                     /locus_tag="ACHOQY_005210"
     CDS             complement(<1..350)
                     /locus_tag="ACHOQY_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9681090.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVK"
     gene            complement(350..>1584)
                     /locus_tag="ACHOQY_005211"
     CDS             complement(350..>1584)
                     /locus_tag="ACHOQY_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681091.1"
                     /translation="VKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSF
                     SGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITG
                     ALWQRTLTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSA
                     NKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQAFDKSAYPKLAAA
                     YPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGT
                     KSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSV
                     GMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAG
                     NAENTVKNIAFNYIVRLA"
BASE COUNT          405 a          402 c          354 g          423 t
ORIGIN      
        1 tctttcaccc aggctgtgcc gttccacttc tgaaactccc cttccggcga taaccaggta
       61 acattttccg gtaacggacc gagttcagaa ataaataacg cgtcccctga cgctacgtca
      121 taaaccgttt taccccgatg gtcttcaacg agatgccacg atgccttatc actgttgaaa
      181 acagccacaa agccagcagg aatatctggt ggtgcaatat cggtactgtt tgctggcaga
      241 cctgtatgag gcggaatata tgcgtcacct tcaccaataa attcattagt tccggccagc
      301 agattataaa tttttatggt ccgtggttgt tcactcattc tgaatgccat tatgcaagcc
      361 tcacaatata gttaaatgcg atgtttttga cggtgttttc cgcgttacca gcagcgttaa
      421 cggtgatggt gtgtccatgt gaaccaatcg caacggagtg cgtatgagca ccaataccga
      481 cagtatgcgc gtgtgcacct gcagatgctg ctgtgccgga cagtgaatgg ctatgattac
      541 catctgtact ggtattcgct aaccaccccg tagacatacc tactgagcct tgtacacccc
      601 aggtattttg acctgagctt gtataaccat attgataagt atctttaaaa acactggggt
      661 taaatcgacg gccatctcta tggctgtgat taccagctgc attcgtgctg ccacttaaac
      721 tatgggtatg cgcaccagtg ttattcgtgg atttagtgcc gtaatcaaac gacgatgtgg
      781 ttttcgtccc caaatccgta ctggatgcgc tggcgctgtg ggtatgcgat ttaatgccgt
      841 cctgttcctg agacaatacg gcccgaccac tggcaggttt gcccttaatc gtccagccac
      901 gcatatcagg gatcacgcct gacggataag ccgctgcaag tttcgggtat gcagatttgt
      961 caaaagcctg cccctgcatc agggcatagc cagacggaac ggtatctgat ggccacggga
     1021 ttggtgcgcc aggcggataa aactgctctg atggcgtata gagtgaataa actgtaccgt
     1081 ccgttaaccc ttccggctta ttagcagaat atgctggtga cgtatgaatc gtcacgctgg
     1141 cattactggt ataatcccat tgaatattta caccagtcgc ataatttccg attgcaacgt
     1201 aaatatcgta agtatcacca gatgtattaa cccaggcaaa atttgtaaac cctgtcaatg
     1261 tgcgctgcca taaagcacca gtaatcccct tcggattacc attacctgca cgtaaaacaa
     1321 gttcagatat acctgcctgt tgaggtgacc ccacgttaaa tccagcgcca ccaatcaacg
     1381 taattgaaac aacagaactc gcctgtggca tggttaccgt tgctaatttg aaccaaccag
     1441 caccaccgct gaatgacatt gttgttgagt taagcgtacc aatatctttc ggcgtcagtg
     1501 ttatatccgc tgaaagcgcc ttaccattca ccttacgggc agaaggtacc cgaccattcg
     1561 cattgtcatt agctgctttc actg
//
LOCUS       DAZKGS010000400         1583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKGS010000400 DAZKGS010000000
VERSION     DAZKGS010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..506)
                     /locus_tag="ACHOQY_005212"
     CDS             complement(<1..506)
                     /locus_tag="ACHOQY_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681092.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIV"
     gene            complement(691..1380)
                     /locus_tag="ACHOQY_005213"
     CDS             complement(691..1380)
                     /locus_tag="ACHOQY_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9681093.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(1395..1517)
                     /locus_tag="ACHOQY_005214"
     CDS             complement(1395..1517)
                     /locus_tag="ACHOQY_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9681094.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          495 a          398 c          274 g          416 t
ORIGIN      
        1 atcacaatct caaaaaaatg tttttttata aatggcttat catagaagca tgataccgat
       61 aaaaatcgcc cataagatct tatcgaaatt gtaaccgcca gcgcaatcgc tgctgacagt
      121 agcaaaagag gtacctgaat cttctgtctc aatatagaaa actcaataat tgccggcaca
      181 aacaataatt ccacagcaaa ataaaggcga aatacattta gctcttgcat agaatgtttt
      241 cttttcactg cgaaaaagaa tacaacacca ataccccaac cgataagaaa tatagcaatg
      301 acgataactg caaaaaataa acttctggca acatcatcaa cacctgcacc tacaatccac
      361 catgggaagc cgtagtaaaa agaagtaccc catccataga aataagcact cccccatcca
      421 aggcatccca tgtaggcaat aaaaagtgaa gaactcctga gcagcgcacc atccttcata
      481 accaccccaa tacaagatga taacattggc ttacaactca taacaaaagc aattcaatgc
      541 cgtcaagagg ttacaggcta aaaaaactct attacatagc agccagcatg tttaccgtac
      601 aagtacaact cagggcataa aaaaaaccca ctcggcagcg ggtttataca ttttttacaa
      661 cataccaaat ttgcatgaag tatatggctt ttaatccagt tttgcaatat tttgctgtaa
      721 aaatgctgcc ttttgttttg aacgtgttct cgtcacaagc aataaagcat cactatcaag
      781 ctgtagaaaa atgtgcttca ttgcaaccca gcgttcagta aatgtctcgg accagttttt
      841 tgttgtcact cccaccaatg atgccagtgt ctggtattca taggcctcac gccctgcaag
      901 ttcgctcttc acatcctgtg cagccagcca gattaacgtc ttcaggcgat ccagtgtctt
      961 acctgcaatt tttctgttac ttaacaaatc tttaaactcg ctccatgccc attgcgttat
     1021 ggtgacctga tgcccccatc gaacgctttc gctgtaacac caaagcaacc atgctttctg
     1081 atgttcatcg agagacaaaa ccgcgcggcg ccatgaagag gttgagaatt caaccgggct
     1141 gaccaaagca atggatgaac cttttgcgta cgactgctta ccggaagtcg gcgtattatc
     1201 cagcgtaatc atcttgccag ttaccacatc cagaatgcgc ggcttctttc gtttgtatgt
     1261 accagtatca aattgtgcat gctcctgcca ggcttcgagc tggcctttcg ttgctccgtt
     1321 caagtcagca gtagctgcca taagttgctc acgaacatac tgtaaatatt gggtattcat
     1381 gcagtaaatc ctttctatat tttggcataa ttcttcaaca ttcggtaatc gttcaaaacc
     1441 gaatcgggga aacgacataa gcacaggagc ccccagcgac agcgaaggag ttctgatata
     1501 taagactcag acatcattca ttccccggtt ctccaatatc tgtttcactc atcatccata
     1561 actacctgta attgcccccc ctt
//
LOCUS       DAZKGS010000401         1544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKGS010000401 DAZKGS010000000
VERSION     DAZKGS010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..453)
                     /gene="sctT"
                     /locus_tag="ACHOQY_005215"
     CDS             complement(<1..453)
                     /gene="sctT"
                     /locus_tag="ACHOQY_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFP9681095.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLW"
     gene            complement(455..715)
                     /locus_tag="ACHOQY_005216"
     CDS             complement(455..715)
                     /locus_tag="ACHOQY_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9681096.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(725..1390)
                     /locus_tag="ACHOQY_005217"
     CDS             complement(725..1390)
                     /locus_tag="ACHOQY_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9681097.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(1380..1544)
                     /locus_tag="ACHOQY_005218"
                     /pseudo
     CDS             complement(1380..1544)
                     /locus_tag="ACHOQY_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
BASE COUNT          536 a          339 c          240 g          429 t
ORIGIN      
        1 ccaaagagtt tccagaataa agttcattcc acctttagta agataaaccg cagcagagaa
       61 aaggttaaat aatcttgcta attccgaggt atcgacacct gtagccggat ccaaagtact
      121 acttaatgtt gcaccgcgtt ggttatcgat aaagctacct atagcaagga aaatccaaaa
      181 cggggatgct aataagcatg caataaacag ccctatgata atttctctgg cagcgaacaa
      241 agcaatatcg atttcattta agttcaataa ttcatagtgg taatgctgaa ctaatgcaca
      301 tgataccact attattattg ggtgacgaac tactgttggt atattcccac tggcgaaaaa
      361 tggtaacaaa tagaaagttg gtgccaatcg gcaaaaaccg agcgctgtag cagccagcag
      421 actatgcaac tgatagagta tcgcctcccc catgctatcc tctcgcgaat gctaaattca
      481 gcatttcgat accaaatgaa tataattttt caccatacca cccgctcatt aaaaaaaagc
      541 aaatacttac gcacaataat ttcacgccaa atggcaacgt ctgttcttgc aactgcgtta
      601 ccgtttgaaa aagccctacc aataaaccaa caaatgtcgc aactgcaata ggtcctgcag
      661 acataactaa aatcagatac agcgccctat tccccgcaaa aacaatatcg tccatatttt
      721 tttctcaggg attaatggat aggtcaaaat attgtaaaat gagccctttt gataacattg
      781 tccaaccgtc catcgcgaca aacaaaatta attttatcgg tgtagatatg gttaccggac
      841 tcatcatcat catacctaac gttaacagaa cgctggatat aaccagatcc actacgacaa
      901 agggtaaata gatataaaaa cctataataa atgcactttt tatctcactc aatgcatatg
      961 cgggcagtaa cgagaaaatt gaaatattat catcatcgat tatctcttca ttgtcttcag
     1021 aactattcac cttctgaatc ttttcaaaaa agctaaccaa ctcaggttca gaatatttta
     1081 tcaaatatga tttataacca ctcatgcctg tctcaacaaa attaacaacc gatgctacat
     1141 tattgaatga aagattctca ttctgactgt tattatatat ttccttccca accggcatca
     1201 ttacgaacat tgacaatagc aaagcaaccc cattaagtgt catatttgat ggcacttgct
     1261 gaagtcccag cgcgtttcgg acaataacaa aaacaatcga gaatttaata aaacatgtcc
     1321 cggatgctat tataaacggc aatagtgtaa ataatgataa tatcgctatc agcgaaatgc
     1381 tatttgacat tattattccc tgataaccaa gaatgaattt ctacgcccaa tttatcatcc
     1441 acttcaacaa gttcgccata gccagttagc gcaccattta cacgtatctc tatattttct
     1501 cagattcagg aagcagagat attattcttt ttgcacagag atcg
//
LOCUS       DAZKGS010000402         1538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKGS010000402 DAZKGS010000000
VERSION     DAZKGS010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1291
                     /locus_tag="ACHOQY_005219"
     CDS             <1..1291
                     /locus_tag="ACHOQY_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9681098.1"
                     /translation="NGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQVAYNKYLVQTA
                     TRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLSNNL
                     GNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFIS
                     IPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQN
                     NDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAI
                     LDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQII
                     APYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIR
                     VDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1304..>1538
                     /locus_tag="ACHOQY_005220"
     CDS             1304..>1538
                     /locus_tag="ACHOQY_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP9681099.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNAL"
BASE COUNT          493 a          304 c          304 g          437 t
ORIGIN      
        1 aaatggttgg aatacaccgc tgggtgccat ctctttttac gctgcccgat cgagtagtaa
       61 gttaaataat gataccagac acgaaggtac gagctatcag gtcgcctata ataaatatct
      121 agtacaaaca gccactcgat ttagcgtggc cgcctggcgt tatgcttcgc aggattacag
      181 gacattcagc gaccatcttt acgaaaatga taaacactat catcagagtg attatgatga
      241 tttttatgat attggcagaa aaaatagcct ttctgctaat attatgcaac ctttatccaa
      301 taatctggga aatgtatcat taagtgcgct ttggcggaat tactgggggc gaagtggaaa
      361 tgctaaagat taccaattca gttactccaa taactggcaa cacattagtt atactttctc
      421 tgccagccaa tcttatgatg aaaataataa agaagaggag cgttttaatc tgtttatctc
      481 cattcctttc tactgggggg atgatattgc caaaacacgt caccaaatta acttatcgaa
      541 ttcgacctca ttttccaaag atggctattc ctccaacaat actggaatta ctggcatagc
      601 cggtgaacat gatcagttaa attatggtat atatgttaat cagcaacaac aaaataatga
      661 tacctcgctt ggtacgaatt taagctggag aactcccatc gccataatag atggcagcta
      721 tagtcattct aaaaacgcct ggcaaagtgg tggaagtatt agtagtggat tagttgtctg
      781 gcccggtggt attaatatca ctaaccagtt atccgataca tttgcaattc tggatgcgcc
      841 tgggttagaa ggtgcacata ttaatggaca aaaatacaat cgaacaaaca gcaaaggcca
      901 ggttgtttac gacctgataa tacctcatcg tgaaaaccat ctggtacttg atatagcaaa
      961 cagtgaaagt gaaacagaat tgcagggcaa tcgtcaaatt attgcacctt accgtggagc
     1021 agtttcttat gtgcagttta caactgacca acgtaagccc tggtatatac aggcactgcg
     1081 tcccgatggt tcgccattaa cctttggcta tgacgtactg gatctccagg aaaataatat
     1141 tggagtcgtt ggccagggta gtcgcctttt tattcgcgta gatgaaattc ccactggcat
     1201 aaaagttgct ctcaatgatg aacagaactt attctgtact attacttttc aacacgttat
     1261 cgatgaaaac aaaacatata tatgccagta aaaggagttt actatgaagt ggcttttatt
     1321 aatcaccttg agtctatatt cttttattgt gcaaagtgct ccctgtgcat tgaccaatgt
     1381 cggtgaacaa cgtggaacat atatactcaa gccactgagt atgaaaggta atctaaccgc
     1441 cgaaaaaatt gaaaacaccg gaaatatcat tgagtgtaca gacttgcaag agacaaccaa
     1501 cgccattaaa gttcattttt taacacagaa cgctttaa
//
LOCUS       DAZKGS010000403         1532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKGS010000403 DAZKGS010000000
VERSION     DAZKGS010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..632)
                     /locus_tag="ACHOQY_005221"
     CDS             complement(<1..632)
                     /locus_tag="ACHOQY_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9681100.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSG"
     gene            complement(632..961)
                     /locus_tag="ACHOQY_005222"
     CDS             complement(632..961)
                     /locus_tag="ACHOQY_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681101.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(958..>1532)
                     /locus_tag="ACHOQY_005223"
     CDS             complement(958..>1532)
                     /locus_tag="ACHOQY_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9681102.1"
                     /translation="QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSA
                     IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIGPAAL
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          316 a          516 c          373 g          327 t
ORIGIN      
        1 ccgctcaggc atttgctgca tttatccttc gtgatatcgg acgttggctg gtcatattca
       61 tccgcgacag ccggaccgct ataaccgcac tcgtcaccgc gataggtcca ggtgcaggtg
      121 ttggccagca tgatacgtcc cggaaaaaca gcgccatccg tttccgtcgg cgtggacagt
      181 acaaaggagg cactcaccgc gctcagttcg ctgcactgct cgatgcgcca gcggctgatc
      241 acctcctgct ccggatcggc gtcactgttt ccgttgacga agttcaccgc atccagaaaa
      301 cgggcgtaaa ccttacgccg gaccaccgtt ccgccgacca gactctgcag atcttccgcc
      361 atcccggtga ccataccgta caggttagaa accgtcagcg tggggcgcgt actggtgcct
      421 ttgccattca gttcaaaacc gctcccctga atgggatacg gctgatactg tcgcccctgc
      481 caggtgaccg gctcaccttt ttcgttctgc tcattacaga aaaaataacg ttcaccaccg
      541 acctctgtca gatcgatttc ccagagcacc acgctggccg actgctccgc acgggtgcat
      601 tcattcagtg tttcctgccg gatatcctgc atcagttcac cacctgttta aactctgcgc
      661 tgaactcaac acgcaacata ctgacctgcg acgaccattt tgcgcaggtc acctttatct
      721 gcctgtaacc ataaggcggc gtccacagaa aggccttcca gcccccgtgc tcagccagaa
      781 acgactccag cgccgtggcc tcctcacggg agacagacag cgtcacgctg tacgttttca
      841 ggtcagcgtt cagcccggca ggcacacgct gggaatagcc atcaccaaag cgcacctccc
      901 tgacggaagg ggccgatgtc acatccatac cgggtttcac tttccagcgg aaggttttca
      961 tcgtccacct ccggagaaca ggccaccatc acgcatctgt gtctgaattt catcacgggc
     1021 acccttgcgg gccatgtcat acaccgcctt cagagcagcc ggacctatct gcccgttcgt
     1081 gccgtcgttg ttaatcacca catggttatt ctgctcaaac gtcccggacg cctgcgaccg
     1141 gctgtctgcc atgctgcccg gtgtaccgac ataaccgccg gtggcatagc cgcgcatcag
     1201 ccggtaaaga ttccccacgc caatccggct ggttgcctcc ttcgtgaaga caaactcacc
     1261 acggtgaaca atccccgctg gctcatattt gccgccggtt cccgtaaatc ctccggttgc
     1321 aaaatggaat ttcgccgcag cggcctgaat ggctgtaccg cctgacgcgg atgcgccgcc
     1381 accaacagcc ccgccaatgg cgctgccgat actcccgaca atccccacca ttgcctgctt
     1441 aagcagaatt tctgtcatca tggacagcac ggaacgggtg aagctgcgcc agttctgctc
     1501 actgccggtc agcatcgccg ccatattctg tg
//
LOCUS       DAZKGS010000404         1527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKGS010000404 DAZKGS010000000
VERSION     DAZKGS010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..627)
                     /locus_tag="ACHOQY_005224"
     CDS             complement(<1..627)
                     /locus_tag="ACHOQY_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9681103.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCL"
     gene            complement(627..956)
                     /locus_tag="ACHOQY_005225"
     CDS             complement(627..956)
                     /locus_tag="ACHOQY_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681104.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(953..>1527)
                     /locus_tag="ACHOQY_005226"
     CDS             complement(953..>1527)
                     /locus_tag="ACHOQY_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9681105.1"
                     /translation="QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSA
                     IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSR
                     IGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNGQIGPAAL
                     KAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          317 a          510 c          368 g          332 t
ORIGIN      
        1 caggcatttg ctgcatttat ccttcgtgat atcggacgtt ggctggtcat attcatccgc
       61 gacagccgga ccgctataac cgcactcgtc accgcgatag gtccaggtgc aggtgttggc
      121 cagcatgata cgtcccggaa aaacagcgcc atccgtttcc gtcggcgtgg acagtacaaa
      181 ggaggcactc accgcgctca gttcgctgca ctgctcaatg cgccagcggc tgatcacctc
      241 ctgctccgga tcggcgtaac tgtttccgtt gacgaagttc accgcatcca gaaaacgggc
      301 gtaaacctta cgcctgacca ccgttccgcc gaccagactc tgcatatctt ccaccatacc
      361 ggtgaccatg ccgtgcaggt tagaaaccgt cagtgtcgga cgggcagcac tgcccttgcc
      421 gttcagttca aatcccgtcc cctgaatggg atacgcctga tactgtcgcc cctgccaggt
      481 gaccggctca cctttttcgt tctgctcatt acagaaaaaa taacgttctc caccgacctc
      541 tgtcaggtcg atttcccaga gcaccacgct ggccgactgc tccgcacggg tgcattcatt
      601 cagtgtttcc tgccggatat cctgcatcag ttcaccacct gttcaaactc tgcgctgaac
      661 tcaacacgca gcatactgac ccgcgacgac cattttgcgc aggtcacctt tatctgccgc
      721 cactcataag gcggcgtcca cagaaaggat ttccagcccc cgtgctcttc cagaaacgac
      781 tccagtaccg tggcctcctc acgggggaca gaaagcgtca cgctgtacgt tttcaggttg
      841 gcattcagcc cggcaggcgc tcgctgggaa tagccatcac caaagcgcac ctttcttaca
      901 gaaggggccg aagccacatc cataccgggt ttcactttcc agcggaaggt tttcatcgtc
      961 cacctccgga gaacaggcca ccatcacgca tctgtgtctg aatttcatca cgggcaccct
     1021 tgcgggccat gtcatacacc gccttcagag cagccggacc tatctgcccg ttcgtgccgt
     1081 cgttgttaat caccacatgg ttattctgct caaacgtccc ggacgcctgc gaccggctgt
     1141 cagccagact gcccggtgta ccgacataac caccggtggc atagccgcgc atcagccggt
     1201 agagattccc cacgccaatc cggctggttg cctccttcgt gaagacaaat tcaccacggt
     1261 gaacaatccc cgctggctca tatttgccgc cggttcccgt aaatcctccg gttgcaaaat
     1321 ggaatttcgc cgcagcggcc tgaatggctg taccgcctga cgcggatgcg ccgccaccaa
     1381 cagccccgcc aatggcgctg ccgatactcc cgacaatccc caccattgcc tgcttaagca
     1441 gaatttctgt catcatggac agcacggaac gggtgaagct gcgccagttc tgctcactgc
     1501 cggtcagcat cgctgccata ttctgtg
//
LOCUS       DAZKGS010000405         1520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKGS010000405 DAZKGS010000000
VERSION     DAZKGS010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(57..1382)
                     /gene="rclA"
                     /locus_tag="ACHOQY_005227"
     CDS             complement(57..1382)
                     /gene="rclA"
                     /locus_tag="ACHOQY_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9681106.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGHQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          408 a          368 c          304 g          440 t
ORIGIN      
        1 ataagattct tttctctgag acgccagaat atttgttctg gcgtctgatt ttgagtttat
       61 ttgactaatg aaaatagatc attgagtgat tcgctcatcg acggatgagt aaatatctga
      121 tcgcgtaata tgctataagg cagcccggca tccatcacca ttttcactat attgatcatc
      181 tcgtgggagt caacacacag cagtgatgcc cctaatatac gttgggtttt attatcaaca
      241 atcgctttta ataccccacg agtatcattc atcactctgg cacgcggaat tgcagctaca
      301 ggcaatgtca ccacctgaat atcagcacca ctctctctgg cctgttcttc tgtcatacca
      361 accctggaca gtggcggtgt catgaatacg gaataaggca catttttccg atcatcagta
      421 ctacgtctgc cttctcccag caactcatca cgcacaatgc ggtaatcatc cagtgaaata
      481 taagtaaatt gcagcccgcc ggtaacatct cccatcgccc aaatattgtc tgcggtggta
      541 tgtaattgct tgtcaacgac aattgccccg cgctcgttta ctgcgatacc ggcattttct
      601 ggatgtaacg aagcggtagc cggttgatga ccggaagcta ttaacagtgc atccaccgcc
      661 agttgggcgt gctcgctatg cacttgcact tgattttcat ggtgactgat tcgctccaca
      721 tgggcattga ggataatatc gacgccctga tcgcgtaaaa tcgtcgcgat attatcagca
      781 atatcccgat cttcccgagg caaaaacagc gaagctgctt ctaaaatggt taccttgctg
      841 ccaaaattag cgaacataga ggcgaactca acgccaatat atccgccgcc caaaatacct
      901 aaatgcccag gcaattcttt tagattaagt aatccggtgc tgtcatatac tcctggcgtg
      961 gtggtaattc caggaattgg cggaaccacg gtttgtgcac cggtattaat aaaaattttc
     1021 tcgccatgaa tttccagatt tgcctcaggc cgatgaacac gcaggctatg attattgata
     1081 aactccgcct ggccgtcgat cacgtcgata ttgggcatat ccgcaagatt atgaaaattc
     1141 ttattacgta aaaaattaac cacttcattt ttacgctgta tggcacggac aaaatctgtg
     1201 tgctgctgtg cgtcatgaac caatgttttg gttgggatgc agccgatatt aatacaggtc
     1261 ccgccataca ttgcatttga ttgttcgatg agagccactc gccaacctgc ttttgccagc
     1321 gtgacggcta atgtttttcc agccttgcca aaaccaataa tcactgcctg atatttattc
     1381 atgatgattt cctgtcgaaa cgcgttcatt tgacatcatg actatagacg caaaagagca
     1441 aataccattt cttcacaacg cttgatattt tgtcttatcg tctggtattt tctttttctt
     1501 aatcagaatc acaaaacaaa
//
LOCUS       DAZKGS010000406         1514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKGS010000406 DAZKGS010000000
VERSION     DAZKGS010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..407
                     /locus_tag="ACHOQY_005228"
     CDS             42..407
                     /locus_tag="ACHOQY_005228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681107.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            407..>1514
                     /locus_tag="ACHOQY_005229"
     CDS             407..>1514
                     /locus_tag="ACHOQY_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP9681108.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRF"
BASE COUNT          353 a          363 c          447 g          351 t
ORIGIN      
        1 gttataaaaa cagacaatat cattgtcttt caggtagtta tatgtcccgt tcagctaaac
       61 cccgtaaacg aaaacctgcc cctcaaagaa gcaaacttcc ccgctatgtc gtgaagcttc
      121 acgacgatga cttctttgac gaagaagacg cagaagctct gcgctttgat aattttgacg
      181 atgccgttga gtgctgcgca gacctgaata ttcccttctt tgtggatgcc ggaaacaaaa
      241 agctggtctt ctggtttgta cgtgttgatg acgaagggta tcctgaaata gcccgctgca
      301 cggagcggga gtttgcgacc attcttgccg gtatcagcgc cggcggcatg tactgcccgg
      361 agtgtggcac ggttcactgg ccggacggag tccccccgcc cttctgatgc ttccccgttt
      421 tgccgacatt tttcagcagg gaaaccgctg gcttaactgg ctggagaaac aaccggaagg
      481 ttcagtgcgt ccggtagtca ttgagtctgt gacaaaaatc atggcctgcg ggaccacgct
      541 gatggggtac acacagtggt gctgttcatc tccggactgc agccacataa aaaaggtctg
      601 cttccggtgt aaaagtcgct cctgcccgca ctgcggagtg aaggctggcg cacagtggat
      661 acagtatctg ctgagtctgg ttcccgactg tccgtggcag catattgtgt tcacacttcc
      721 ctgccagtac tggtccctgg tgttccacaa ccggaggtta ctggcagaga tgagccgcat
      781 tgctgcggat gtgatacagg aaatctgccg ccaggcagat gtggtgccgg ggatattcac
      841 ggtgatccac acatggggac gtgaccagga gtggcatccg cacattcacc tgtcgacaac
      901 gaccggcggc gtgacatcag accacacctg gaaaaacctt catttttacg cccgtaaggt
      961 gatgagtatg tggcgttacc ggataacgcg gttactgtca cggaaatatc cggacctggt
     1021 gataccggat gcgctggcag cagaaggaag cagtaaacgg gactggaatc gcttcctgga
     1081 cagtcattac cggcggggct ggaatgtcaa cgtatcccgg gtgatggata acgccacaca
     1141 tgtggcggtg tacttcggct cttacctgaa aaaaccgccg gtgccgatga gccgtctgga
     1201 gcactatgct ggtcaggatg aaattggtct gcgttacaac agtcaccgga caaaacggga
     1261 agaatacctg gtgatgagtg gtgatgagtt tatggaaagg ttctcctggc atgtggcgga
     1321 taaggggttc cgtatggtga ggtactacgg tttcctgagt ccggtgaagc gccggttact
     1381 ggaagatgtt gtgtacgtca taacggagac ggtgagaaag acggcgatgc aaatcaggtg
     1441 gagagggatg tatcagcggt tactgaaggt tgacccgctg aagtgcatcc tgtgcggagg
     1501 tcagatgcgt ttta
//
LOCUS       DAZKGS010000407         1509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKGS010000407 DAZKGS010000000
VERSION     DAZKGS010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..106)
                     /locus_tag="ACHOQY_005230"
     CDS             complement(<1..106)
                     /locus_tag="ACHOQY_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9681109.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNM"
     gene            complement(139..438)
                     /locus_tag="ACHOQY_005231"
     CDS             complement(139..438)
                     /locus_tag="ACHOQY_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9681110.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(577..810)
                     /locus_tag="ACHOQY_005232"
     CDS             complement(577..810)
                     /locus_tag="ACHOQY_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9681111.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            924..>1509
                     /locus_tag="ACHOQY_005233"
     CDS             924..>1509
                     /locus_tag="ACHOQY_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9681112.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQD"
BASE COUNT          417 a          314 c          344 g          434 t
ORIGIN      
        1 gcatatttga tagtctggcg taaccatcat cgagatctgc cacattacgc tcctgttcgg
       61 caaagttacc tctgccgaag ttgagtattt ttgctgtatt tgtcataatg actcctgtgg
      121 attgatccag taattccctc agaattccat ctggatttgt tcagaacgct cggtcttgca
      181 caccgggcgt tttttattgg tgagtccatc aagcgcatac ttaaaagctc tgctaatcgg
      241 actgatgtct gatgccattc cgaaagcaca caagaccgaa gcaataaatc tccagtccgt
      301 tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct gggtaagcgt
      361 tgacaggttg ataagtaaat ctgtttctgc gcgatcaaca tcacgctgtg atagtttgct
      421 gtaacttgtt tgttccattt cttaagattt ccaatagtga atagctagtt gaaaggtatg
      481 cgtggaaacg catatggcct tagttggtca gatatcttgg aactcgcttt tcagcgacgt
      541 aggacgaatg tccgttgtta caaagagcgg ggttacttat gctgctgatg ctctacgcga
      601 tacgaacacc agattttcct ttttcacagg tttataaccc gtgaaattac gagtggcttc
      661 ttcgattgca ttcgctttat ctggggaagc tcttcgaaac ccatatacaa tctgatcaag
      721 atagccaact gaagtattcg ctaatgcggc gagtcgcttc cattcctcac tagaagcctc
      781 ttttcgccag cgtagtagtt cattactcat tagtgcctcc gtttatcaca cagagcgact
      841 ttaccatttt gataaataaa acgcaatgaa gatttatcaa aatgcatatt tatccatttg
      901 ctaaatagag ggagaatcgt gggatggaaa acaaagatat tcgcaaatcg aatctggcgt
      961 ttttgctaga tgagcataaa aaaatcgcgg gtaacactaa tgcaagcttt gccgataagc
     1021 ttggagttag cccttctcaa ctcacgcaag tctccggtga aaaaagcact cgaaacatag
     1081 gggataaact agcaagaaaa ttcgaagccg cacttgggtt acctaatggg tggcttgatt
     1141 tggtacatga tgtaacacca attgcatcat gctcagattc tttaactttt gtcggtcagg
     1201 taagaaaagg gttagtgcgc gtggttggtg aggcaattct tggtgttgat ggtgccatcg
     1261 agatgaccga agagcgcgat gggtggctca atatttatag cgatgatcca gatgcctttg
     1321 gtcttcgtgt gaaaggagac agcatgtggc ccagaataaa atcaggagaa tatgtactca
     1381 ttgagcctaa caccaaagta ttcccggggg atgaggtgtt tgtcagaact gttgaaggac
     1441 acaacatgat caaagttctt ggctatgaca gagatggaga ataccaattt acaagcatca
     1501 accaggacc
//
LOCUS       DAZKGS010000408         1503 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKGS010000408 DAZKGS010000000
VERSION     DAZKGS010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..771)
                     /locus_tag="ACHOQY_005234"
     CDS             complement(73..771)
                     /locus_tag="ACHOQY_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9681113.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(771..1112)
                     /locus_tag="ACHOQY_005235"
     CDS             complement(771..1112)
                     /locus_tag="ACHOQY_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681114.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(1105..>1503)
                     /locus_tag="ACHOQY_005236"
     CDS             complement(1105..>1503)
                     /locus_tag="ACHOQY_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9681115.1"
                     /translation="LGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKG
                     AGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGP
                     EAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          317 a          542 c          342 g          302 t
ORIGIN      
        1 cacgactccg caggcacaca ccgccgggca tgcgccagaa tcgctgattc agtctgtgtc
       61 ataaaccggg atttactgcg aaagtttatt aatggaaagg aaaccgccaa aattagccac
      121 catgccgcgc atctcacacc cgcgcatgca cttgctgcat ctgtccttac ggatatccgt
      181 agtggggttg tcgaactcat ccgccaccgc aggaccgtta tacccgcatt catctccccg
      241 gtaatcccac atacaggtgt tcgccagcat gatgcgacca ggaaacagcg ccccgtccgt
      301 ctcggtcggt gttgccagca caaacgaggc tgtcatggcc gtcagctctg acatctgctc
      361 caccacccag cggtcgctca gctcctgctc cgggtccgct tccggattgc ccgccacaaa
      421 attcaccgca tccagaaaac gggcatacac ccggcggcgg accaccgtgg cccccaccag
      481 gctctgcagg tcctccgcca tcccggtgac cagaccaaac agattcgaca ccgtcagcga
      541 cgggcgggca ctgctgccct tcccgttcat ctcaaagcca ctgccgtcaa tcgggtatgc
      601 ctggtacttc cgcccctgcc aggtcaccgg ctcccctttt tcattcagct cattacagaa
      661 aaaataccgc tcaccaccct gggccttcag gtcaatttcc cagagcacca cccgcggtga
      721 ctgctctgat ttaaccgact cgttcaggct ttccccgtga atatgctgca tcagttcacc
      781 acctgctcaa tcgtacagct gaaatcactg taccggacgt tatccgtgac gctccactcc
      841 cggcacacca ccctcaccgt ccggttatgt ttcggcggtc gccacaaaaa ggcacggtaa
      901 ccaccatgcc aggataaaaa ttcatccagc cagcgccggg ttgactcatc cgtcacccgg
      961 aacaccgcct gaaacgtctt cagttgagga ttcagtcctg tggggcgacg ctgttcataa
     1021 ccgtcaccaa accgtaccct cgccaccgac ggtttctcac tcacctgcat cccttcacgc
     1081 gggaccagat gcagcgtttt tatctcagcc actcagcatt cctccgtcac gtcgcatgga
     1141 caacatcacc gcctgcaccc gctggtcaat cagctgcaca agactgcctg ccgcctccgg
     1201 ccctatctgg ccattagtcc cgtcattctg aatggcgata tggtagaccg gggaatacac
     1261 caggcccgca ctaccgttca tactgcccac cgctcgcaca cccagcgagc catccgccgc
     1321 ccgggtcagg ggcataatgg cttcaggacc ggcctccccc atcagtcccg ccccttttgc
     1381 aaaggcaaag tacgtgggcg tatccacaat actgttgctg tacgcactca ggtttgccga
     1441 ggtatacacg ccgccttttg cattggccac cgccccgccc agccagtcac caatgctgcc
     1501 gag
//
LOCUS       DAZKGS010000409         1497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKGS010000409 DAZKGS010000000
VERSION     DAZKGS010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..382
                     /locus_tag="ACHOQY_005237"
     CDS             <1..382
                     /locus_tag="ACHOQY_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9681116.1"
                     /translation="SWAVNHYRDNAIAYKEQRDKATSIIADMQKRQRDVAELDARYTK
                     ELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSMGDGESPGLTSDAELNYYRL
                     RGGIDKITAQVNYLQEYIRTQCLK"
     gene            532..1149
                     /locus_tag="ACHOQY_005238"
     CDS             532..1149
                     /locus_tag="ACHOQY_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9681117.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(1337..>1497)
                     /locus_tag="ACHOQY_005239"
     CDS             complement(1337..>1497)
                     /locus_tag="ACHOQY_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9681118.1"
                     /translation="RNIRFPNQMIEQINIALEQKGSGNFSAWVIEACRRRLTSEKRAY
                     TSIQSDDG"
BASE COUNT          408 a          337 c          380 g          372 t
ORIGIN      
        1 gtcatgggct gttaatcatt accgtgataa cgccatcgcc tacaaagagc agcgcgataa
       61 agccacatcc atcatcgctg atatgcagaa gcggcaacgt gatgtagcag aacttgacgc
      121 cagatacaca aaggagcttg ctgatgctaa tgcgactatc gaaagtctcc gtgctgatgt
      181 ttctgctggg cgtaagcgcc tgcaagtctc cgccacctgt gcaaagtcaa cgaccggagc
      241 cagcagcatg ggcgatggag aaagcccagg acttacatca gatgctgaac tcaattatta
      301 ccgtctccga ggtggaatcg acaagataac cgcgcaggtt aactacctgc aggaatacat
      361 caggacgcag tgcttaaaat aattttaatt tcactgaaat ttaacaagtg actttcagga
      421 aaatgcctcg cagatgcggg gcatttttgt accggtattt caccgcgcac cgcagcgcac
      481 aataaacacc gaacctgacc ctttggaatg ggcctttgag gataccagtt agtgctggcg
      541 agcctcggtg ggctggtttc ctgtgcggca aaggttcatt tcaaagaagc aggcaacgcc
      601 atgaatgaat taattgcgaa tcatgacttc gactttcgcc agttagttac cgcagcagaa
      661 ggtcaaccgg taactgacac cttccagatt gccagggcat ttggtaaacg ccatcagcat
      721 gtgattaggg ctattaaatg tttgagatgt tctgaggaat tctcgacaac ccatttttgg
      781 gccgtcgaga aaatcaatga cttaggtatt tttgacaaga aacagattta ctaccgcatg
      841 gactttagtg gcttcgttat gctggttatg ggatttaacg gggcaaaagc cgatgctgtt
      901 aaagaagcct atatcaatgc gtttaactgg atgtcagcag aactccgtaa gtacagcgaa
      961 agttatgaag cagaacgtaa cgccgtaatg ctggagtaca tgaaagagaa ggatgtcgcc
     1021 agcatgtcag gccgtctgct caatcgctgg gggagaacga aaaaacctca attgcttgca
     1081 aagctggaac gtctggagag acagggacag tttttattac cgggattcga taaaggtatt
     1141 caagcctgac acattatgcg ctgtatcgtc gccgtattcc cgcattaacc atgaccgtag
     1201 cccgacgggg aattccttct gcgtgagtgt gcgggaataa tcaaaaacga tgcacaccgg
     1261 gttttactgt gctgacagac gcagggttac cctcatagtc gcttttccgg tgcgatggtg
     1321 gaagaaaccg ggatgttcat ccatcatcac tttggattga tgtatatgct ctcttttctg
     1381 acgttagtct ccgacggcag gcttcaatga cccaggctga gaaattccca gacccttttt
     1441 gctcaagagc gatgttaatt tgttcaatca tttggttagg aaagcggatg ttgcggg
//
LOCUS       DAZKGS010000410         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKGS010000410 DAZKGS010000000
VERSION     DAZKGS010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..370
                     /locus_tag="ACHOQY_005240"
     CDS             23..370
                     /locus_tag="ACHOQY_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFP9681119.1"
                     /translation="MKVKKVQLLVTFLSMFSFSAVAMPFKTIERESFNGVWPFNTDEV
                     QLQCLDGNPYVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSL
                     GDVTKAAFDLCDK"
     gene            376..1377
                     /locus_tag="ACHOQY_005241"
     CDS             376..1377
                     /locus_tag="ACHOQY_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681120.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPQKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
BASE COUNT          425 a          242 c          371 g          444 t
ORIGIN      
        1 tgagctaaat tgaggtaatg atatgaaagt aaaaaaggtt caactattag ttactttttt
       61 atctatgttt tctttttccg ccgtcgcaat gccttttaaa actattgaac gtgagagttt
      121 caatggggta tggccattta atactgatga ggttcaatta cagtgtcttg atggtaatcc
      181 ttatgtgatg aattttgacg ataataagtt atatgcgctt acaggtttag ctcgaataaa
      241 aggtaaaaca tttggtgcgt taccgttaga taacaataat ccattttggc tagataatga
      301 tgccgcccca gggttaaaaa agagtctggg ggatgtcact aaggctgcat ttgatttatg
      361 tgataagtaa ctaaaatgtc ggttcgtaag attccatcag gtaaatggct ttgcgaatgt
      421 tatccctacg gggcatcggg aaaacgcatt cgtaaacagt ttgcgacaaa aagtgaggcg
      481 ctctcttatg agcgccgttt aatgaatagt agagttggag acgagtttca agatggttct
      541 ggtcctcgtc tttctgagtt gattgctcgt tggtttgaga tgtacggtaa aaccttgtcc
      601 tctggtgcag agcgcaaagt caaacttgag gcgatttgtt ccaggctggg ggatccattt
      661 gcttctcagt ttgacaaaaa tatgtttgct acttatcggg aaagaaggct atcaggagaa
      721 tggaatccca aggggaagaa aaaacttagt gaagcaaccg ttaatcgcga gcagtcatat
      781 ctacatgctg tttttgccga actgaagcgc cttggggagt ggtctggtga aaaccccctg
      841 actggtattc gcaagtttcg tgaggaagaa aaggaactgg cgtttctgta tgtagatgag
      901 attgaacgcc ttctgattgc gtgtgatgag tcacggaata aagatttggg ggttgttgtc
      961 cgtattgggc ttgcgactgg tgctcggtgg agtgaagctg aaggattaaa gcaatctcaa
     1021 gtactgcccg gtcgaatcac atttgttaaa actaaaggaa agaagaaccg cactgtaccg
     1081 atttcacctc aattgcaggc tatgcttcct caaaaacgag gagcgctatt ttcaccatgt
     1141 tatgaggctt ttgacgctgc aattaagaga gcgaagatcg agcttcctga tgggcaatta
     1201 actcatgtgc tacgtcacac gtttgccagt cattttatga tgcggggcgg aaatattctt
     1261 gtgttgcaaa aaatactggg gcatagcgat ataaaaatga ctatgcgtta tgcgcatttt
     1321 gctccaggtc atttagaggc tgctgttgaa ttgaaccctt ttgacaatag agggtaaaaa
     1381 gtggcgataa aaagtgacaa tagatttaaa tggcgttgta tagagccatt ttcaaagtta
     1441 ataaaatcat aaggttatga tttataaatg gtggttgata gt
//
LOCUS       DAZKGS010000411         1464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKGS010000411 DAZKGS010000000
VERSION     DAZKGS010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..270)
                     /locus_tag="ACHOQY_005242"
     CDS             complement(<1..270)
                     /locus_tag="ACHOQY_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681121.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHOQY_005243"
     CDS             complement(365..571)
                     /locus_tag="ACHOQY_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9681122.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHOQY_005244"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHOQY_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          378 a          340 c          414 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tggtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZKGS010000412         1461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKGS010000412 DAZKGS010000000
VERSION     DAZKGS010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..178
                     /gene="ydcD"
                     /locus_tag="ACHOQY_005245"
     CDS             <1..178
                     /gene="ydcD"
                     /locus_tag="ACHOQY_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFP9681123.1"
                     /translation="NISKLENMEFCATKQRVYFSDKNKKASYKIINYGDYYDVDYYDN
                     NLKNEVFDWIGKWS"
     gene            complement(93..278)
                     /locus_tag="ACHOQY_005246"
     CDS             complement(93..278)
                     /locus_tag="ACHOQY_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681124.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFSNCYHNNQHRNSLHN"
     gene            360..1461
                     /locus_tag="ACHOQY_005247"
                     /pseudo
     CDS             360..1461
                     /locus_tag="ACHOQY_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          542 a          195 c          341 g          383 t
ORIGIN      
        1 aaacattagt aaactggaga atatggagtt ctgcgctaca aaacaacggg tgtattttag
       61 tgataaaaac aagaaggcta gttataaaat aattaattat ggagactatt acgatgttga
      121 ttattatgat aacaatttga aaaatgaagt ttttgactgg attggtaaat ggtcataatg
      181 aacaacacca gaaagagaaa aatataaaat acatacagaa agacatgaga gcaagggacg
      241 gggcagggca agatcatgaa aattgtgatg taaatcacga ttttcatctt ggctttaacg
      301 cctacaggtg atcaaaattt aacctttgga ataactaaaa agataaaaaa ggacgccagg
      361 tgagtattca aagtttgctt gattatattt cagtgatccc tgatatacga caacaaggaa
      421 aggttaaaca taaattatct gctattttgt ttctcaccgt atgtgcagta attgcaggtg
      481 ccgatgagtg gcaggaaatt gaagattttg gacatgaaag acttgaatgg ctaaagaaat
      541 atggtgattt tgataatggc attccggtcg atgacaccat tgcacgcgtt gtgagtaaca
      601 ttgacagttt ggcctttgaa aagatgttta ttgaatggat gcaggagtgc catgaaatca
      661 ctgatggtga aattatagca atagatggaa agaccataag aggctccttt gataagggaa
      721 aaagaaaagg agcaatccat atggtgagtg cattctcgaa cgaaaatggt gttgtactgg
      781 ggcaggtgaa aacggaagcc aaaagtaatg agattacagc cattccagag ttgcttaacc
      841 tactgtattt aaagaaaaat ttgataacca ttgatgctat gggctgtcag aaagatatcg
      901 cttcgaagat caaagataaa aaagcagatt atcttctggc agtaaaaggc aatcagggga
      961 aattacatca tgcattcgag gaaaaatttc ctgtaaatgt gttttctaat tataaaggcg
     1021 attcgtttag tacgcaggag ataagtcatg gaagaaaaga aacacgtttg catattgtca
     1081 gtaacgtaac gcctgaactt ttgtgatttt gaattcgaat ggaagggatt aaaaaagctt
     1141 tgtgtagcat tgtcattcag gcagaagaaa gaagataaat cagcagaagg tgtaagcatc
     1201 cgatattata tttcatcaaa ggatatggat gctaaagaat ttgcacatgc tatcagagcg
     1261 cactggctga tcgagcacag tcttcattgg gtgttagatg taaaaatgaa tgaagatgcc
     1321 agccggataa gaagaggaaa cgcagcctaa ataatatctg gaataaagaa gatggcactg
     1381 aatttattaa gggattgcaa agacattaag ggtgaagagg aaaagaaaga aggctgcgtt
     1441 aaacacaggg aaagatcatc a
//
LOCUS       DAZKGS010000413         1458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKGS010000413 DAZKGS010000000
VERSION     DAZKGS010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..394
                     /gene="ydeI"
                     /locus_tag="ACHOQY_005248"
     CDS             2..394
                     /gene="ydeI"
                     /locus_tag="ACHOQY_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP9681125.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            649..1260
                     /gene="dgcZ"
                     /locus_tag="ACHOQY_005249"
                     /pseudo
     CDS             649..1260
                     /gene="dgcZ"
                     /locus_tag="ACHOQY_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
     gene            1315..>1458
                     /locus_tag="ACHOQY_005250"
     CDS             1315..>1458
                     /locus_tag="ACHOQY_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9681126.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTAS"
BASE COUNT          383 a          300 c          345 g          430 t
ORIGIN      
        1 tatgaaattt caggccattg tattagcaag ttttcttgtc atgccttatg cgttggcaga
       61 tgaccagggc ggtttaaaac aggatgcagc gccaccgccg cctcatgcaa tagaagatgg
      121 ctatcgcgga accgatgatg caaaaaaaat gaccgttgat ttcgcaaaaa acatgcacga
      181 tggcgcttcg gtttcactac gcggtaattt gatttctcac aaaggagagg accgttacgt
      241 ttttcgcgat aagagcggtg aaattaatgt cgttattcct gcggccgtct ttgatggacg
      301 agaagttcag ccggaccaga tgatcaacat tagcggcagt ctggataaga aaagcgcgcc
      361 ggccgtcgtt cgggtcactc atttgcagaa ataaaaataa attagcctga tggcctgaac
      421 tactgggcca ttagggtatt gggttatgct atttgtcatc cttttattta cgataatgca
      481 gaggtgattt acagaattaa acactttttt atccgctgat tttgttttaa atcacaatgt
      541 tatctcagag atgactcata acttcggatt tctatgaaaa tattgacgta attttgcttt
      601 tctctcgtta gaatagcgcg cacaaggaac tgtgaaaaag gagtggcaat gatcaagaaa
      661 acaacggaaa ttgatgccat cttgttaaat ctcaataagg ctatcgatgc ccactaccag
      721 tggctggtga gtatgtttca cagcgtggtc gcgagagatg ccagtaagcc agaaataacg
      781 gataaccatt cttatggact gtgccagttt ggtcggtgga ttgatcatct ggggccactc
      841 gataacgatg aattacctta cgttcggcta atggattctg cccatcaaca tatgcataac
      901 tgtggtcggg aattaatgct ggctattgtt gaaaatcact ggcaggacgc gcatttcgac
      961 tcctttcagg aggggttgct ttcttttact gcggcattaa ccgattacaa aatttatttg
     1021 ctgacgatcc gtagcaatat ggatgttttg acgggattgc cggggcgtcg ggttcttgat
     1081 gaatcctttg atcatcagtt acgcaacact gagcctctga atctttattt aatgttgttg
     1141 gatattgacc gatttaaatt ggttaatgat acctacgggc atttaatcgg cgatgtagta
     1201 ttacgcaccc tggcaactta cttagccagt tggacgcgtg attacgaaac ggtttatcgg
     1261 gtaatgctgc caacttactg atttagtgta tgatggtgat tttaaggtgc ttgcgtggct
     1321 tccatttcca tcagatgtcc ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa
     1381 agcactgccg gacatcagcg ctatctctgc tctcattgcc gtaaaacatg gcaactacag
     1441 ttcacttaca ccgcctct
//
LOCUS       DAZKGS010000414         1453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKGS010000414 DAZKGS010000000
VERSION     DAZKGS010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..359
                     /locus_tag="ACHOQY_005251"
     CDS             78..359
                     /locus_tag="ACHOQY_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9681127.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            325..1401
                     /locus_tag="ACHOQY_005252"
     CDS             325..1401
                     /locus_tag="ACHOQY_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681128.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT          503 a          288 c          321 g          341 t
ORIGIN      
        1 agctgattat taaaaatcaa ccacacccgc cagtattctg tatatttact ggcggtcata
       61 tcgtaagagg tatggcaatg aatcttgtga cactcaaaac gtggggaaaa ctcagatatc
      121 cggataaccc accatcaata tcaacgctga gacgatgggc aaggaatgga aacatttatc
      181 ctgcacctga actacacggg aggagttaca gggtggttcc ggaagctttc tatatcaacc
      241 caaataaggt tgataccgat ataacacacc atcagcctaa tgggcgacaa gggagagaca
      301 gtccgttact ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttacctaaa
      361 aacttaacat atcgaaggag ggacaaagca tattactggc gcaaccctct gacgaaagaa
      421 gaatttacac taggtaaaat ttcaagaaga gatgcagtag cgcaggcaat tgaagcaaat
      481 cattatatat acaaaaacta ctctcctgct gccttaattg aaaaactaaa agggttcgac
      541 tcatttacta tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg
      601 tccagaaata cttataaaat tcgggtaaat caactggaga caataaaaga aaaattagga
      661 ggggttttac tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt
      721 gaaggaggga aaaacacgat ggcaggatca atgcgttctg tgttatctga tatgttccgc
      781 gaggccattg ttgaaggacg tatatctcaa aatccagtaa cgccaacaag agcaccgaaa
      841 atagtagtta caagagaacg gctgaaacta aagacataca actgcatcag ggaggcagca
      901 gatcaacttc cgacatggtt cccattagct atggacttag cccttgtaac aggacaacgt
      961 cgcgaagaca taacaaatat gcggttcagt gagatttatg atgatcgtct ccacatcagg
     1021 caaattaaga caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt
     1081 ttacgacttg gtacagtagt tgaacagtgc cgcatggtaa gtagggggga ttatctaatc
     1141 agtgccggga ttagaaaaaa cagccctgac ggcagcattc acccggacgg cctgacaaag
     1201 aaatttgtcg cagccagaaa attaacaggt atccagttca gtgaaaaccc accaactttt
     1261 cacgagatca gaagcctggc tggacgattg tacaaagaaa catgtgggga agaatttgct
     1321 caacgtctac ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa
     1381 aaaacgtact tactgctctg attttaacgt aaatggattg ttaaatgtgt tttggttgtg
     1441 atataaccaa aaa
//
LOCUS       DAZKGS010000415         1445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKGS010000415 DAZKGS010000000
VERSION     DAZKGS010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1445)
                     /locus_tag="ACHOQY_005253"
     CDS             complement(<1..>1445)
                     /locus_tag="ACHOQY_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9681129.1"
                     /translation="YVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEIDKLDNDSQID
                     NDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDTFIFTII
                     PDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDITYKVNGLSIIPENFFTLLSGNFK
                     ADWRWDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVL
                     YALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELLNTRDMILNELHKH
                     VFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMESNKDSRINKNVVVLAG
                     NHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCHYDSERKVLTSHHGIIRDEENACY
                     CLGALQVPFKQMKNPTDPEELANIFNKKHKEHMNDPLLHLIRSNIMKPTAVYADYINN
                     TTDFRPKPERIFICGETLKCEDPSIYIRQKYG"
BASE COUNT          446 a          260 c          236 g          503 t
ORIGIN      
        1 ccatattttt gtctaatgta tatgctggga tcttcacatt tgagtgtctc tccacatatg
       61 aagatacgtt caggttttgg tctgaaatca gtagtgttat taatataatc agcatataca
      121 gctgtgggtt tcataatatt gcttcggatt agatgtaata gaggatcatt catatgttct
      181 ttatgttttt tattaaatat atttgctaat tcctcaggat cagtaggatt tttcatctgt
      241 ttaaatggaa cctgcaaagc accgaggcaa taacatgcat tttcttcatc tcttattatt
      301 ccgtgatgac tggttaaaac tttcctttct gagtcataat gacaaacatc gagcgtttta
      361 attaagttat acgtatcacc ggcagagagt ttatgatttg ctagtcgtgc agtatagtta
      421 ccattaaaat tgatttcatg atttccagcc agaacgacga catttttatt aatacggcta
      481 tctttattac tctccatatt tcgcatagaa tttagcaaag taaggatata tttatcacca
      541 aaaatagtac taaatctatc gcctgtatga tccccaagga agatacaagg ggttatcgca
      601 tcgtccttta aaaaaacatg tttgtgcaac tcattcaata tcatgtctct cgtatttaat
      661 aactcgtaga gcactttatt atttttatgc ttgtatgcag atgaagccat gttctcgtga
      721 tttaataatt cacataataa actccaaccc ttttccctga tgcctatatg ccctgaaacg
      781 agcaatgcat aaagtacagc gcctactctt ccatctgtat cgccaaaata agccgtgttg
      841 gcattttgtg ggttaatcac accacattgt ttataaaagt ctattttaga aaacacagat
      901 ttaacatagc cagcaaactt atccttagga aacgtctctt tagagacatc ccaacgccag
      961 tcagctttaa aattaccgct aagaagagta aagaaatttt caggtatgat agaaagccca
     1021 ttaactttat aagttatgtc tgatttatct ccagaggaat taataaaata ataaccgccc
     1081 ttacactcaa cagtaacggt aatgggagac gataatttta acacatggtt attgtctggt
     1141 ataattgtaa agataaacgt gtcagtagta ttgtcggcga atctattata ttcttgaata
     1201 tctatagaag gttcactatt gtaaaaccat actttctcca tctgatgact acgatcattt
     1261 accctccagt gaccatgttg caggaaatag atgatatgca tgccaaaatc gttatctatt
     1321 tgcgaatcat tatccagttt atcaatttca actatctttg aacgatgtgt tttatcaacc
     1381 ttgctatggt gttgatgatt attaacttta tctgaatgct tatctttatg gtttgaaggg
     1441 acgta
//
LOCUS       DAZKGS010000416         1435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKGS010000416 DAZKGS010000000
VERSION     DAZKGS010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..531
                     /gene="espL2"
                     /locus_tag="ACHOQY_005254"
     CDS             <1..531
                     /gene="espL2"
                     /locus_tag="ACHOQY_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFP9681130.1"
                     /translation="HADAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLLLAYQNG
                     DINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYGKLFKKL
                     DTSPYKMSIILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFNICPEKVAEYLS
                     EFSGKHFLDVYNYYSN"
     gene            713..898
                     /locus_tag="ACHOQY_005255"
     CDS             713..898
                     /locus_tag="ACHOQY_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9681131.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            1139..>1435
                     /gene="nleB"
                     /locus_tag="ACHOQY_005256"
     CDS             1139..>1435
                     /gene="nleB"
                     /locus_tag="ACHOQY_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9681132.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYE"
BASE COUNT          506 a          227 c          268 g          434 t
ORIGIN      
        1 catgcagacg caataaaggc atattgtggc gtacttggca atagtaattt gaaaagaggt
       61 gagataatca ggatgcttga ggcgagaaat tatgatggtg caccaggatt acttcttgcg
      121 tatcaaaatg gagatattaa tacgattcag tctttttttg actcactaat aatgttagat
      181 atatcaaagg attttatcga agagttattg actgcaaaac attatgattt tacggggtta
      241 tcattagcga tcagtcatag acatgatcat gttgtcaaat tatacggaaa gttatttaaa
      301 aaacttgata ccagtccata taaaatgtca attatcctag cattagcaat tgattgcgag
      361 agaaataatg caaacataat tattgatagt gagtacaagt caaataaggc agtaaaggaa
      421 tatgtagaaa ttttaaaaga attcaatata tgcccagaaa aggtagcaga atacctaagc
      481 gagttcagcg gaaaacattt cctcgatgta tataattatt attccaattg aaaaaaatat
      541 aaagtatcta tcacggcact ctcagttgag tatcacaggc acatgtattt tagccccctg
      601 agtctacaga caaccaatat tacttaaata agtgataatc ttaatgctgt tttttagaac
      661 agatgattga tatttttggg ccggagaaac gcagacaggc tttgttgaat aaatcgagct
      721 tttgctgagt ttaaggatca gatcactcat tttcctgcaa aagctcattg ggcgaaacaa
      781 tctgactcag agaacccgga ttaaacgtct ggcccgcaga accatctatt tctcacgcac
      841 tgtagtacgt aataaaaaag tcatcggtac ttttatcaaa aaacatatat tctattaatt
      901 attgccatca cctttttctt attgacaaca ttactctgct gattgttaaa atctaacctg
      961 tattcagggt tacgattcta tacaatagaa cagggagggg aaaggatgtc atcgttaatg
     1021 agactttttg ttaatgtcaa caatgctatg aatttatttt agaaaaacaa ttaagctgac
     1081 gtagtgttcc gtctaaatga cggggcgata ttaacatgat tagaacaaga ggaattttat
     1141 gttatcttca ttaaatgtcc ttcaatccag cttcagagga aagacagcct tatcaaatag
     1201 tacacttctc cagaaagttt cttttgctgg aaaagaatat cctctggaac ctattgatga
     1261 aaaaacccct attctttttc agtggtttga agcaaggcca gagcgatacg aaaaaggaga
     1321 agtaccaata ttgaatacca aagaacatcc gtatttgagc aatattataa atgctgcaaa
     1381 aatagaaaat gagcgtataa tcggtgtgct ggtagatgga aattttactt atgaa
//
LOCUS       DAZKGS010000417         1434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKGS010000417 DAZKGS010000000
VERSION     DAZKGS010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1434
                     /locus_tag="ACHOQY_005257"
     CDS             <1..>1434
                     /locus_tag="ACHOQY_005257"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9681133.1"
                     /translation="KTQEVLENNFWVNFDIVFNSKDIPIVNEYSSDLSDEHLLSSHII
                     YSNIQKVTSSRGNPLISRVPPDFFDLIIIDEAHHAPAQSWRDTLHYFSNAKILHVTGT
                     PYRGDNQEIPGVKIHETPLSEVMRDKYVKWLRKETVNAHDLFFYTPEQPGVKLSKEQV
                     LMFKDKEWIEKSIALSKECSLDVINHSIKKLNELKELSPNVPHKILAIGCSISHAEDL
                     LSWYKAKGMTSVIVHSEMDQNDLSDALMKIEHHQCDVVISVNMLMEGYDHKYLTVLAI
                     FRPYRSLNAFAQVVGRILRAIPESEITHFEIDNNGIVIFHEEIGLNVMWETFQKEVER
                     ARRASVREYTFTDRDYHERSNTLAGIESDSAYVSDTDSYLDDVDFNAMFEQKRNEINN
                     SVEQQIEKIKSSGVDLPEDALEALRQTLAEKAVQKAAEIIDPELIEKRPHQARKLMRN
                     ILKTRTQDGVSNLLIDNGIGEKSSELY"
BASE COUNT          521 a          235 c          269 g          409 t
ORIGIN      
        1 aaaaacgcaa gaggttttag aaaataactt ctgggttaat tttgatattg tttttaatag
       61 caaagatata ccaatagtta atgaatactc atctgattta tctgatgaac atcttttaag
      121 cagccacata atttattcaa acatacaaaa agtaacatct tctcgaggca acccactaat
      181 aagtcgtgta ccccctgact ttttcgatct cattattatt gatgaggctc atcatgcccc
      241 cgcacaaagt tggcgggata ctcttcatta tttttccaat gctaagatat tgcatgttac
      301 tggcactcca tatagaggcg acaatcaaga aataccagga gtaaaaatac atgaaactcc
      361 tttatctgaa gttatgcggg ataaatatgt caaatggtta agaaaagaaa ccgtcaatgc
      421 acatgattta ttcttttaca cgccagaaca gccaggagta aagttatcta aagaacaagt
      481 tttgatgttt aaagataaag aatggataga gaaaagtatc gctctttcca aagagtgctc
      541 tttagatgtt attaatcata gtataaaaaa acttaatgaa ctaaaagagc tgtcaccaaa
      601 tgtcccacat aaaattttgg ctataggctg tagtatatct catgctgaag atttactttc
      661 atggtataaa gccaaaggaa tgacatcagt aattgttcat agtgaaatgg atcaaaacga
      721 cctttctgat gctctaatga aaatagaaca tcatcaatgt gacgtcgtta tatctgtcaa
      781 catgctcatg gaagggtatg atcataaata tctaacagta cttgctatat ttagaccata
      841 ccgtagccta aacgcttttg cacaagttgt tggacgcatt ttacgagcga ttcctgaatc
      901 agaaattacc cactttgaaa tagataacaa tggaatagtc atattccatg aggaaatagg
      961 tttaaatgtc atgtgggaaa catttcagaa agaagtggag agagcacgca gagcaagtgt
     1021 aagagaatat acatttacag acagggatta tcatgaaaga tctaatacat tagctgggat
     1081 agaaagtgat agtgcttacg taagtgatac agactcttac ctagatgatg ttgactttaa
     1141 tgctatgttt gaacagaagc gtaatgagat caacaattct gttgagcaac aaatagaaaa
     1201 aataaaatcg tctggagtag atcttcctga agatgctctt gaagcattac gccaaacatt
     1261 agcagaaaaa gctgtgcaaa aagccgcaga gataattgat cctgagctaa ttgaaaaaag
     1321 gcctcatcaa gcgagaaagt taatgagaaa catcttgaaa acccgcactc aggatggtgt
     1381 ttctaattta ttgattgata atggaattgg tgaaaaaagc tccgaactgt atag
//
LOCUS       DAZKGS010000418         1431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKGS010000418 DAZKGS010000000
VERSION     DAZKGS010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            185..1330
                     /locus_tag="ACHOQY_005258"
     CDS             185..1330
                     /locus_tag="ACHOQY_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFP9681134.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          487 a          295 c          248 g          401 t
ORIGIN      
        1 tgctgatgaa tcaatgaaaa tctattgttc gtcattagag gagtatttcc tctaatttaa
       61 aaaaataact ctctatacat ttaaaactct cgctataaat agcttatata ttattgacac
      121 tatatcatgc tatatgatat ctgttgtcga atcattcata ataaatgaga caggggaatc
      181 tctcatgata aacggactta ataataattc cgcatcttta gttttagatg ctgcaattag
      241 aattaattct gattttaaaa agcagtggaa tgatatgtca tgcgctgaaa aattactaaa
      301 agttcttagt tttggtttat ggaatccaac gtatactcgt agtgaaaggc aaacatttca
      361 agagttgtta accgttttag aacctgtatc tcccgcgcct aatgaattgg gcagaatata
      421 tgctaatttt gcagatggct catccttaag aatttccgtc actaacagcg aacttgttga
      481 agccgagatt cgcacaccag ataatgaaaa gattcttatg ctcctggagt caaacgaaca
      541 aaataggtta ttacaatctt tacccatcaa tctccacatg ccatacattc aggttcatcg
      601 tgccttatct aagatggacc tgactgatca taagtcaatg cacaatctac ttagttttac
      661 gtcaaaacta tcagcaacct tgattcctca taatactcaa acagatccgc tttccgggcc
      721 tacaccattc agctctatgt ttatggatac atttcgagga ctcggcaatg caaagctttc
      781 actcaatggt gttgatatac ctgtagatgc acaaaaattg cttcgcgatg cactaggact
      841 taaagacaca cattcatcac ttgcccggaa tgttataaat aatggtattt ctcgtcatca
      901 tgcaaagcaa atagcaaggg aaagcagcgg cagcgataaa cagaaagctg aagttgtgga
      961 gtttttatgc caccccgaag cggcaacagc catatgctcg gctttctatc aatctttcaa
     1021 tgtgccagcc ttaatgttga cacatacaag gatctctcaa gccagagaat acaatgtgga
     1081 aagatcgtta gatgtaccta acgcgtgcat taacataagt atctctcaat caccagatgg
     1141 aagcattcac gttgccagcc ataccgggat tctgataatg gcgccagaag accgccccaa
     1201 cgagttagga atgttgacga acaggacctc ctatgaagtg ccacaaggtg tgaaatgtga
     1261 aatcgatgag atggtaagaa cgctacaacc aaggtatggc gcatctgaaa catacctgaa
     1321 aaacatttaa aaataaaaaa atggacacca cacactttat ttatcaaaac aaacaataac
     1381 taatcacact cacaattatt aataatttaa cctcacagca atcatacaaa a
//
LOCUS       DAZKGS010000419         1428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKGS010000419 DAZKGS010000000
VERSION     DAZKGS010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            221..868
                     /locus_tag="ACHOQY_005259"
     CDS             221..868
                     /locus_tag="ACHOQY_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9681135.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRILAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(1128..1347)
                     /locus_tag="ACHOQY_005260"
                     /pseudo
     CDS             complement(1128..1347)
                     /locus_tag="ACHOQY_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          421 a          243 c          329 g          435 t
ORIGIN      
        1 ggatgaagta acccgacagc ttcaaaaaac cggagtctgg ctccggtttt ttgttgtcat
       61 gtcatggtga tgtttgttaa tgaagttaaa tttattttgg cttgataatg gagtcttatc
      121 ttttgcaaga ctgataatgg tgtgtgaaag cattgtgata agtaagatgc ttcaacagga
      181 aatcaagtaa aatgttaatg aagttaatgg gggttttatt atgccaataa atttaacacc
      241 atatctaaca gcgagtgggg gactggggga aataccgcag gatactcttt ctggcatacg
      301 aatactggct tttaacggag gagctcaggt tcagatgggg agcactattg ttacaatacg
      361 ttctgtcttt ttaggcttct ttatgggaag tgtaagccct gaaggattat cagagagagc
      421 tttacgaact gcattgaata atgttaatcg tcttgaacgc gaccttaatg gagggctatc
      481 cggccgccag attttagcaa gtatacccag cccgtatgct tctccacctc gtccttctgt
      541 aatgcaaaca gagttggtgt tagagcaaat agaaaaatgc tcatttaatg ttaactcgtc
      601 atctctgagg gcgactgaag aggcattaac atgcccgatt acattatgca tccctgagca
      661 tggtgtgatt atgagaaatg ctggagattc agatgtatgt actttatatg ataaagaatc
      721 attaaaacat cttgttaaca caagttcacc tcatcctctg agcagagaaa aaataacaga
      781 gtcaatgatt gttaaggaaa actcgtgcta ttttgatttc acatcaggaa gtattaaaaa
      841 tgttcgtggt gagataacta gactataaga tgttagaaca tattgactga taagtgtaat
      901 tcacagacat cttttcagtt ttttctgtta attttctaat tgatggactg tatacaggga
      961 taaaaggaga gggtattgtc tggacatttc tgttagcata attggaaatg tgcgtgaaaa
     1021 aaagcataac agacatcatg tgttatgtat gtattcagag tgcccataat tcaccagtgc
     1081 agggataaat tgtaaaaaaa tcgcagtacg tcgtatgcag aacggtgcgg cgtacgcagt
     1141 tccagtggac cgtcgcctgt gataacggtc tttgtggaat aaccgttgcg ggagttagct
     1201 cctgatctgg actgattttt ctcattccca agatggtgtg tcatctctgc attgagagcg
     1261 gcttcaacgc tgagcttttt cagcaaccga tcaaactgac tgaggtcttc tgagggggct
     1321 gaggtttttg gccagttcgt tagcaataca ccagaaaaat tctaaacgag cctgtacata
     1381 gatttgtgta attgcctgat tttgatatgt tcaacccaac atcaaatg
//
LOCUS       DAZKGS010000420         1410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKGS010000420 DAZKGS010000000
VERSION     DAZKGS010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1410
                     /gene="rhsB"
                     /locus_tag="ACHOQY_005261"
     CDS             <1..>1410
                     /gene="rhsB"
                     /locus_tag="ACHOQY_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFP9681136.1"
                     /translation="GRLTEKTDLIPEGVIRTDDERTHQYHYDSQHRLVHYTRTQYAEP
                     LVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRI
                     QTIYQPGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRL
                     ESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISK
                     EGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFD
                     SIDSTAYDTLNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPN
                     GTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHG
                     KEITNRKGLPNVCRVHGNM"
BASE COUNT          401 a          333 c          393 g          283 t
ORIGIN      
        1 acggcaggct gacagagaaa accgacctca tcccggaagg ggttatccgc acggatgatg
       61 agcgcaccca ccagtaccat tacgacagtc agcaccggct ggtgcactac acgcggacac
      121 aatatgcaga gccgctggtc gaaagtcgct atctttacga cccgctgggc cgcagggtgg
      181 caaaacgggt atggcgacgt gaacgggacc tgacgggctg gatgtcgctg tcacggaaac
      241 cggaagtgac ctggtacggc tgggacggcg accggctgac cacgatacag aacgacagaa
      301 cccgcatcca gacgatttat cagccgggga gcttcacgcc actcatcaga gttgaaaccg
      361 ccaccggtga gctggcgaaa acgcagcgcc gcagcctggc ggataccctt cagcagtccg
      421 gcggcgaaga cggtggcagt gtggtgttcc cgccggtgct ggtgcagatg ctcgaccggc
      481 tggaaagtga aatcctggct gaccgggtga gtgaggaaag ccgccgctgg ctggcatcgt
      541 gcggcctgac tgtggagcag atgcaaaacc agatggaccc ggtgtacacg ccggcgcgaa
      601 aaatccacct gtaccactgc gaccatcgcg gcctgccgct ggcccttatc agtaaggaag
      661 gggcaacaga atggtgcgca gaatacgatg agtggggcaa cctgctgaat gaagagaacc
      721 cgcatcagct gcagcagctt atccgcctgc cggggcagca gtatgatgag gagtccggcc
      781 tgtattacaa ccgccaccgc tattatgacc cgctgcaggg gcgatatatc actcaggatc
      841 cgattggact gaagggggga tggaacctgt atggatatca attgaatccg atatcagaca
      901 tcgaccccct gggtttatct atgtgggagg atgcaaaatc gggggcatgt actaatggtc
      961 tttgcggcac actatccgct atgataggtc cagataaatt tgattctata gatagcaccg
     1021 catatgacac cttaaataaa ataaatagcc aatctatttg cgaagataaa gagttcgctg
     1081 gtttaatatg taaggataat agtggcagat atttctcaac agcacctaac cgaggagaaa
     1141 gaaaaggatc atatccattc aatagccctt gccctaatgg tactgagaaa gtatcagctt
     1201 atcatactca tggtgcagat agtcatggag aatattggga cgaaatattt tcaggtaaag
     1261 atgagaaaat agttaaaagt aaagataaca atatcaagtc attttattta ggtacgccca
     1321 gtggtaattt taaagcaata gataaccacg ggaaggaaat aacaaacaga aaaggattac
     1381 ctaatgtctg cagagttcat ggtaatatgt
//
LOCUS       DAZKGS010000421         1403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKGS010000421 DAZKGS010000000
VERSION     DAZKGS010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1126)
                     /locus_tag="ACHOQY_005262"
     CDS             complement(<1..1126)
                     /locus_tag="ACHOQY_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681137.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPN"
     gene            complement(1072..1293)
                     /locus_tag="ACHOQY_005263"
     CDS             complement(1072..1293)
                     /locus_tag="ACHOQY_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681138.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
BASE COUNT          276 a          461 c          341 g          325 t
ORIGIN      
        1 cattgggcgt gagaagcgta tttttcaccg catcccccag cgttttcatg cgccgctcac
       61 tgtccattct cagcagcgtg gtgacatcaa attctgtact ctcgttttcc cccgtttcca
      121 gcgcctcatc cagtaacagt tcaatggact caatcagcgt ctgcaggcac tgggaataat
      181 actgctgctc cagcgcctcc acgttgtcac tggaaggcgg ttgtcccacg ccaatcttgt
      241 aggccgggac acggaacacc gaacagacaa tttcagcggt catcttcagt tgttccaccg
      301 tctgcgcatc cacaggtgaa aacgtcgtgg ggttgtattt tgccccgttg ctcagaatgg
      361 ccgttttccc cgcattttcg cctgtatacc cgctgtccca gttgctcttc agttttttcg
      421 cattttcttc cgtaatactg ccggggatct caatcacccc ggacggcctg ccgccatttc
      481 tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg cgtggccgcc agcccggcga
      541 catacaccgg cggcaatcct ataagcggat gaaaaaaaca gttaaaccgg tcgtggatca
      601 cttcccgggc aggcaccgtc accgcctccg tgatcccgca gttccggtcc ggcgtgatgc
      661 ggtagaacac ctcgccgtca tccgccacca gaggttcaac ccggctccag tccagaatac
      721 gcagttcttt gatctgcccc cgggcattac ggattttcag caccaccgta ttgccatgac
      781 gcagtttggc gttcagccac agttcaaaaa actggatgcg gttctgctgg gcgttgggac
      841 gacgacagag gcgggcaata tccccccggc gcgtttccct gcgtatccca tgcgcatccg
      901 tctgcataag acgcagccgc attttggcga tatcctggga tatcagcgaa atacatgcaa
      961 acaccgcatg aaaggagagg acggcttcag gatcggcttt cacgccctgc tgccaggcgc
     1021 cggaaaaggg ctcagccacc gcctgaaaca ggctggtcca gcccgcctct cttacgtcac
     1081 gtcctgattt ctggtttttt cgggttcgcc gtaaaaggtt ccacattcgc catgctccgc
     1141 atcacgtttc tttttctgac ctgccggacg tcgcaccgtg atgtactccg cctttcccag
     1201 gcgaaccagc acctccgcac acggctgtgc cacatcacgg atatccccgg cccgggcatc
     1261 atgcgtgccc tgcagatatc ggatctttgc cataacctgt tacgggaggc gcacgcctcc
     1321 cgtcctcctt atcagactca gccgccggac gcactgccgt agttcactcc ggtgatcacc
     1381 gccaccgcca caccgccgtc atc
//
LOCUS       DAZKGS010000422         1395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKGS010000422 DAZKGS010000000
VERSION     DAZKGS010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1395
                     /locus_tag="ACHOQY_005264"
     CDS             <1..>1395
                     /locus_tag="ACHOQY_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9681139.1"
                     /translation="GSAGVRIGAPTGVACSVCPGGMTSGNPVNPLLGAKVQPGETDLA
                     LPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDIRLQIRDDALILNDNGGRSIHF
                     EPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWASLPPDIRLSPHLYLATNSAQG
                     PWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGRTLAYRREAAGDLAGEITGVTD
                     GAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPLSAPSFPDTLPGTEYGADSGIR
                     LSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYDRSGTQMRHFTYDDKYRGRMVG
                     HRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAG
                     LKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDGRET
                     KFYYNDGNQLTAVV"
BASE COUNT          261 a          413 c          492 g          229 t
ORIGIN      
        1 agggttcggc aggtgtaaga attggcgcgc caactggcgt ggcgtgctcg gtgtgtccgg
       61 gcgggatgac ctcgggcaac ccggtgaacc cgctgctcgg tgcaaaagta cagcccggcg
      121 agacggacct tgcgctgccc ggcccgctgc cgttcattct ctcccgcacc tacagcagct
      181 accggacgaa gacgcctgca ccggtgggcg ttttcggccc cggctggaaa gcgccttctg
      241 atatccgctt acagatacgc gatgatgcac tgatactcaa cgacaacggc gggcggagca
      301 ttcactttga gccgctgctg ccgggggagg cggtgtacag ccgcagtgag tcaatgtggc
      361 tggtgcgtgg cggtaaggca gcgcagccgg acggccacac gctggcacgg ctgtgggcgt
      421 cgctgccacc ggatatccgg ttaagcccgc atctttacct ggcgaccaac agcgcacagg
      481 ggccgtggtg gatactgggg tggtctgagc gggtgccggg tgctgaggac ttactgccag
      541 cgccgctgcc gccgtaccgg gtgcttaccg ggatggcgga ccgcttcggg cggacgctgg
      601 cgtaccggcg tgaggccgcc ggtgacctgg ccggggaaat caccggcgtg acggacggtg
      661 ccgggcggga gttccgtctg gtactgacca cacaggcaca gcgggcggaa gaggcccgta
      721 aaccgcacac cgcttcgtta tcttcccctg acagtccccg ccctctatca gctccttcct
      781 tccccgacac actgcccggt acggaatacg gcgctgacag cggtatccgt ctttcggcgg
      841 tgtggctgat gcacgacccg gaatacccgg aaaatttacc tgccgcgcca ctggtgtgct
      901 acgactggac gccacgcggt gagctggcgg cggtgtatga ccgcagcggt acgcagatgc
      961 gccactttac ttacgatgat aaataccggg gccggatggt ggggcaccgt tatgcaggca
     1021 ggccggagat gcgctaccgc tacgacgatg cggggcgggt ggtggagcaa cttaacccgg
     1081 caggcctgag ttaccgctac cagtatgagc aggaccgcat caccgtcacg gacagcctga
     1141 accggcgtga ggtgctgcat acagaaggcg gggccgggct gaagcgggtg gtgaaaaaag
     1201 aactggcgga cggcagcgtc acgcacagcg ggtatgacgc ggcaggaagg ctcacggcgc
     1261 agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtcc ggcgatatca
     1321 cggacatcac cacaccggac gggcgggaga cgaaatttta ctataacgac gggaaccagc
     1381 tgacggcggt ggtgt
//
LOCUS       DAZKGS010000423         1381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKGS010000423 DAZKGS010000000
VERSION     DAZKGS010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            73..636
                     /locus_tag="ACHOQY_005265"
     CDS             73..636
                     /locus_tag="ACHOQY_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9681140.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            633..>1381
                     /locus_tag="ACHOQY_005266"
     CDS             633..>1381
                     /locus_tag="ACHOQY_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9681141.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDP"
BASE COUNT          329 a          360 c          432 g          260 t
ORIGIN      
        1 gcggcatgag acgccgggaa aaatgggctg aaccataccc ggctgagtgc gttctggacc
       61 cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg
      121 aaaaacaaaa cagcgtcagc ccttgccgcc ggttattcgg cgaagacagc cagggttaaa
      181 ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta tagcccgtaa acagtgcgag
      241 acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagcc ggaggataaa
      301 ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg aaacaaatcc ggagatgcca
      361 ccgcccgtgg tgatatctcc tggtattgag tatatggagg acggtcttcc cgatccggtg
      421 aaagcgatgg ggcgtcttct ggtggagaac attaataccg accccaggct ggcgctggat
      481 gcggcttata agctggcgca attcacgcac cacaaaaaag gggatgccgg taaaaaatcg
      541 gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg cggtgccacc accaccccgc
      601 ctggtggtga ataatgataa tgagggcaac ggatgatacc tgtgtggagc acggcctgcc
      661 cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggctccg atttatccgg
      721 agcaggccgc acatgccctg gcgattttta aacaactgcg gattgtggat gcaccgggca
      781 gcccgacgtt cggggagtcc tgcgcacagt gggtgtttga cctggtggcg gccctgtttg
      841 gctcctacga tgcgcagacc ggtgtacgcc atatcaagga agtttttatc cttatcccca
      901 agaaaaacag caagtccacg ctggctgccg ggatcatgat gacggcgctg ttactgaact
      961 ggcggcaggc ggcgggctac accattctgg ccccgaccgt ggaggtggcg gctaacgcct
     1021 tcaaccctgc cagggatatg gtacgacggg acgatgatct ggatgacctc tgtcaggtgc
     1081 agacacatat ccggaccatc acccacaggg tgacggacac cacccttaag gtggtggctg
     1141 ccgatccgaa tacggtatcc ggtatcaagt ccgtggggac gctgattgat gaactgtggt
     1201 tatttggcaa gcagtacaaa gcggaggaca tgctacgtga agccataggc ggccttgcct
     1261 cccgcccgga agggtttgtg gtgtatacga ccacccagtc gaatgagccg ccagccgggg
     1321 tgttcagaca gaaactgcag tacgcccggg atgtccgtga cggcaaaatt catgatccgc
     1381 a
//
LOCUS       DAZKGS010000424         1370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKGS010000424 DAZKGS010000000
VERSION     DAZKGS010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(576..>1370)
                     /locus_tag="ACHOQY_005267"
     CDS             complement(576..>1370)
                     /locus_tag="ACHOQY_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681142.1"
                     /translation="PAFTELAPRTQKDYRQHQKALLMVFGKVLADNVKTEQVRIFMDK
                     RGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFSLKARTVYITDEQYAAIY
                     AEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQNKTGTKQIKEWSPRLRT
                     AIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAAEQKVGVPFGCNFHDIKA
                     KGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLPLVVSK"
BASE COUNT          363 a          304 c          296 g          407 t
ORIGIN      
        1 ccctaaatag agaagttaaa taatcaggat gatgctaatg aatatcaggg acaaggtgag
       61 tgtcttcatg ttcagtgaaa cagggtgtca caaaaaagat tggggggggc tagagccttc
      121 tgcacgaaaa tagtgatgcg acagatctga gatagctttg tatccttctg cctggttcag
      181 gcctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
      241 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
      301 ccaattggta taccaagttt ataccaagag ccaaaaaagc aaaggggcta ccatttggta
      361 accccttgat tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
      421 ggttttcaag accgaagaaa aacaaataaa aatcagtgca atacgtgaaa ttgctgaaaa
      481 gttaatcaaa tttaacacca agaaaaatca aggtattagg aagttgctca tagctagtat
      541 accaagtctt tttgccagaa atttttctgg attgtctact tgctaaccac gagcggcaaa
      601 tccagtgttg gtgtgatttt tgttttacga tcgtaaatca acacctgatt ttctgtttta
      661 tgcccgctga aaatttgttt gtcgcgactg ctgccttcgt aatctgagat ccccttggct
      721 tttatgtcgt gaaaattgca cccgaacgga acgccaactt tttgctcggc tgcgcgttta
      781 gcctgattcc accagttatt cagcgtttta gctatgactt tcccgccttt ggttgtattg
      841 atcacatatt cgcatgtaca ggaagataca tttcgggcta actggatcgc tgtacgtaat
      901 cgcggtgacc attccttgat ttgtttggtg ccggttttgt tttgctcaat gtagatccct
      961 ttatccataa tatcctgcca tttcaactca agcacatcac cgagtcttgc cgcacagaga
     1021 taggaaatct ccattgcaat gcgtaactgt ggaattgctt ccgcatatat cgccgcatac
     1081 tgttcatcgg tgatgtaaac agtgcgggct ttaagagaga attttctgac tcctttgcat
     1141 gggttattct tcacataccc acgctcatat ccccacccgt atactcgact caggcttgcc
     1201 agttcatgat ttgcctgggt cttgctctca agccctcgtt tatccatgaa aattcttacc
     1261 tgctcagttt tgacattatc agcaagcact tttccgaata ccatcagcaa cgccttctga
     1321 tgttgtcgat aatctttttg ggttcggggg gccagttctg taaatgcagg
//
LOCUS       DAZKGS010000425         1363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKGS010000425 DAZKGS010000000
VERSION     DAZKGS010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..907
                     /gene="espB"
                     /locus_tag="ACHOQY_005268"
     CDS             <1..907
                     /gene="espB"
                     /locus_tag="ACHOQY_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP9681143.1"
                     /translation="NSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEIQKLLGKM
                     VRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLGILGSFAA
                     INSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAVEDASSVMQQAMTT
                     ATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVDKITGSTPFIAV
                     TSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEVRRAQDDIASRL
                     RDMTTAARDLTDLQNRMGQSVRLAG"
     gene            914..1321
                     /gene="cesD2"
                     /locus_tag="ACHOQY_005269"
     CDS             914..1321
                     /gene="cesD2"
                     /locus_tag="ACHOQY_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP9681144.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
BASE COUNT          389 a          276 c          325 g          373 t
ORIGIN      
        1 taattctgtt tcggagaata ctaccggctc taatgcaatt accgcatctg ctattaattc
       61 atctttgctt accgatggta aggtcgatgt ttctaaactg atgctggaaa ttcaaaaact
      121 cctgggcaag atggtgcgta tattgcagga ttaccaacag caacagttgt cgcagagcta
      181 tcagatccaa ctggccgttt ttgagagcca gaataaagcc attgatgaaa aaaaggccgc
      241 tgcaacagcc gctctggttg gtggggctat ttcatcagta ttggggatct taggctcttt
      301 tgcagcaatt aacagtgcta cgaaaggcgc gagtgatatt gctcaaaaaa ccgcctctac
      361 atcttctaag gctattgatg cggcttctga tactgcgact aaaacgttga ctaaggcaac
      421 ggaaagcgtt gctgatgctg ttgaagatgc atccagcgtg atgcagcaag cgatgactac
      481 agcaacgaga gcggccagcc gtacatccga cgttgctgat gacattgccg attctgctca
      541 gagagcttct cagctggctg aaaacgctgc agatgccgct cagaaggcaa gtcgggcaag
      601 ccgctttatg gctgcagtag ataagattac tggctctaca ccatttattg ccgttaccag
      661 tcttgccgaa ggcacgaaga cattgccaac aacggtatct gaatcagtca aatctaacca
      721 tgagattagc gaacagcgtt ataagtctgt ggagaacttc cagcagggta atttggatct
      781 gtataagcaa gaagttcgca gagcgcagga tgatatcgct agccgtctgc gtgatatgac
      841 aacagccgct cgcgatctca ctgatcttca gaatcgtatg ggtcaatcgg ttcgcttagc
      901 tgggtaattg atcatggtcg atacgtttaa tgatgaagtg cttaatcact atcttgaaca
      961 aaaagggtac acaatacaga aggagtttct ttgtggcagt gcctttttta tcggatggcg
     1021 gattgagacc tcttttttct cattagcgta cagacttgat gaacaagaac tgattttgtg
     1081 ctcttttgaa gcacgtaacc aaacagggct taacggccct gttttatcac tgactcgctt
     1141 gctcgaagag ttgtaccacc atttttcggg tattaagaaa atcagtgcga tgaaatctaa
     1201 gattggttca gattcagaac gtcaaaagcg cgaagagttg tttaattact tcatcagaaa
     1261 gggcgctgtg cagcaagaaa cagaagacgg aatttggttc gtaatgaatg taaatagtta
     1321 gaaattttta tttctattaa ctattgagga aaatttaatg aat
//
LOCUS       DAZKGS010000426         1354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKGS010000426 DAZKGS010000000
VERSION     DAZKGS010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..1354
                     /gene="nleA"
                     /locus_tag="ACHOQY_005270"
                     /pseudo
     CDS             47..1354
                     /gene="nleA"
                     /locus_tag="ACHOQY_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
BASE COUNT          434 a          279 c          282 g          359 t
ORIGIN      
        1 tggattgata ttattaatgg atataaacat gtaataagga tttattatga acattcaacc
       61 gaccatacaa tctggaatca cctcacaaaa caatcaacat catcaaacag aacaaatacc
      121 ctctacacaa ataccgcaat ccgaattacc tctaggatgc caagctggat ttgttgttaa
      181 tattccagat gatatacagc aacatgcacc ggaatgcggt gaaacaacag ctctactgag
      241 cttgataaaa gataaaggtc tgctctcagg gctagacgaa tatatagctc ctcaccttga
      301 agaaggatcc ataggaaaaa aacattggat atgtttggtt tattcaatgt tacccaaatg
      361 gcattagaga tacctagttc cgtttcaggc atctctggta aatatggtgt ccagctaaac
      421 attgtaaaac cagatattca tcctacatca ggtaattatt ttttacagat attccctctg
      481 catgatgaaa taggttttaa ttttaaagac cttcctggcc cgttaaaaaa tgcattaagc
      541 aacagtaata tatcaaccac tgcagtgtcg actattgcat cgactggaac atcagccact
      601 acttcgacgg taaccaccga gccaaaaggc ccaataccat ggtttggatt aacagctcaa
      661 gtggttcgta atcatggtgt agaacttcct atagtcaaaa ctgaaaatgg atggaagctt
      721 gttggagaaa caccacttac tcctgatggg ccgaaagcaa attacacgga ggagtgggtt
      781 atcagaccgg gagaagcaga ttttaaatat ggtgcatctc cattacaggc aactctaggg
      841 ctggagtttg gcgcacattt caagtgggat ttagataacc ctaatactaa atatgccgtt
      901 cttaccaatg ctgccgcaaa tgcgcttggt gctttagggg gatttgcagt atccagattt
      961 gctagtacag atccaatgtt aagtcctcat atcggtgcaa tggttgggca agcagcaggg
     1021 catgccatac agtataatac ccctggatta aagccagaca ctattttatg gtgggctggt
     1081 gcgacactgg gggctaccga tttaaacaag gccgagtttg aagtagctag attcactgac
     1141 tatcctcgta tatggtggca cgcaagagaa ggagctattt tccccaataa agcagatatt
     1201 gaacatgcca caggtgctga tatacgcgca atggaagaag gtatccctgt tggacagcgg
     1261 catccaaatc cagaggatgt ggtaatcgat atcgaaagca atggcttacc acatcataat
     1321 ccatcaaatc atgttgatat ctttgatata atcc
//
LOCUS       DAZKGS010000427         1353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKGS010000427 DAZKGS010000000
VERSION     DAZKGS010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHOQY_005271"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHOQY_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9681145.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          403 a          250 c          277 g          423 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata aga
//
LOCUS       DAZKGS010000428         1352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKGS010000428 DAZKGS010000000
VERSION     DAZKGS010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..500
                     /locus_tag="ACHOQY_005272"
     CDS             <1..500
                     /locus_tag="ACHOQY_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9681146.1"
                     /translation="RSKKHGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDT
                     AKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILW
                     DSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLAEYARAL
                     SGEDE"
     gene            497..703
                     /locus_tag="ACHOQY_005273"
     CDS             497..703
                     /locus_tag="ACHOQY_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9681147.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            700..>1352
                     /locus_tag="ACHOQY_005274"
     CDS             700..>1352
                     /locus_tag="ACHOQY_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681148.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQ"
BASE COUNT          333 a          353 c          406 g          260 t
ORIGIN      
        1 aacgctcgaa aaaacatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
       61 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
      121 tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
      181 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatcttaccg
      241 aagcgcagca actgactgct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
      301 tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt tatgcgctgg
      361 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg ctggcgagcc
      421 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagaatac gcccgtgcct
      481 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
      541 tgacctgatg acaggaaaac gggtggcaac ggtacagaaa gacggacggc gagtggagtt
      601 tacgaccact tccgtgtctg acctgaaaaa atacattgct gagctggaag tgcagaccgg
      661 catgacacag cgacgcaggg gaccagcagg attttatgta tgaaaatgtc caccattccc
      721 acccttctgg ggccggacgg catgacatcg ctgcgtgaat atgccggtta tcacggcggt
      781 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag tgtggatgca
      841 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
      901 ggctatgccg ccaacgccat ccagttgcat caggatcata tcgtcgggtc ttttttccga
      961 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     1021 tcccgcgagg ttgaagcggc atggaaagag tttgccgaag atgactgttg ctgcattgac
     1081 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     1141 aacggtgaac tgttcgttca ggccacctgg gatacccgtc cctcgcgact gttccggaca
     1201 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccag cgacagccgg
     1261 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     1321 gaggacgggt atcctggctg gatgccgcag aa
//
LOCUS       DAZKGS010000429         1349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKGS010000429 DAZKGS010000000
VERSION     DAZKGS010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..347
                     /locus_tag="ACHOQY_005275"
     CDS             <1..347
                     /locus_tag="ACHOQY_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9681149.1"
                     /translation="GSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQML
                     APKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEG
                     GDGKVVVIGRQA"
     gene            complement(538..1065)
                     /gene="sodC"
                     /locus_tag="ACHOQY_005276"
     CDS             complement(538..1065)
                     /gene="sodC"
                     /locus_tag="ACHOQY_005276"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9681150.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSTGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          322 a          317 c          390 g          320 t
ORIGIN      
        1 tggggtcgtt cttcacggcc ggggcatcaa tggcgttatg gggttcagcc ctggcagccg
       61 gtggtttttc tgccaccacg atgctgtttt ctctgggggc cagcatgatt ctgggtggtg
      121 tggctcagat gctggccccg aaggctaaag taccggagta caaaagcacg gataacggca
      181 gacagaacac gtacttttcc tcgctggata acatgattgc ccaggggaac ccgatgccgg
      241 tgccttacgg tgaaatgctg gttggctccc gccgtatatc ccaggacatc agtacccgtg
      301 atgaaggcgg tgacgggaag gtggtggtta tcgggcggca ggcataaaag cgaaaaaatc
      361 ccgcagtgct cacggacagg aactgcggga gagttacgaa gattgagtgt aaggaattat
      421 tcttatgtca cgacaaaaac attaactcag agagggagga tgtgccgttc ttttcaggga
      481 gaaaggattt atcgtcctga ggaataaagg taaggggccc gccccttacc tgactgatta
      541 ttgaatgatg ccgcaggcca ttctcgcacc accaccgccc aggggctccg gatggtcatg
      601 atggttatca ccgccagcat gaagcatgag agaacgccct ttaatctctt ttaatgagtt
      661 cagtctcggg gccaggaccg ggtagttcgc ttttccgtca tgcgtcacga acagcgcagg
      721 gaggtcgccc aggtgtccat ccggagacca ggggccaaga tgtttgccgg tgtttttcgg
      781 gtcaaagtga ccgccagccg ataatgctgc gaccggtttt ccgtctttca gtgccggggc
      841 gcaatttcct ttttcgtgca catgaaaacc atgaatgcct tcagacagag agtgaagggc
      901 tggtgtgaac agcagaccgt agggggtctc ctgaatggtt atttttccag tgctgacttc
      961 ttttccgtca gcactgacaa ggttcattgg gacttcctgt tctgctgcgt atccgcatga
     1021 tgctgctgtc agcatggcaa tggcagcaat gattttacat ttcataaaac cctcattaat
     1081 tccgttaaca gactgagctt gctggttaca gggtaacaaa cagcgttctg atgatatcgc
     1141 gcaatagctg tgcaatatcc tatcactgcg attaataata ccaattgaga ggaacattat
     1201 gggtaaaggt ggcggcaggg cgcacacacc tcgtgaggcg aaggacaatc tcaaatccac
     1261 gcagatgatg agcgtgattg acgccattgg tgaagggccg attgaaggtc cggtgaaggg
     1321 gctgcagagt atcctggtga acaaaaccc
//
LOCUS       DAZKGS010000430         1332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKGS010000430 DAZKGS010000000
VERSION     DAZKGS010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHOQY_005277"
     CDS             <1..99
                     /locus_tag="ACHOQY_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681151.1"
                     /translation="FIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(135..1277)
                     /locus_tag="ACHOQY_005278"
     CDS             complement(135..1277)
                     /locus_tag="ACHOQY_005278"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFP9681152.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          419 a          262 c          218 g          433 t
ORIGIN      
        1 tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa tttattaggc
       61 gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa ggctgcataa
      121 tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct ttgagttacg
      181 ccatcccaac tgtagtgttc aaacacaaag tcctgacctt gctttgcaac tgctgttaat
      241 tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt cataggttct
      301 tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc acgcgtactc
      361 actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa tggttcctgg
      421 aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatgcat cttttcaggc
      481 ggtatgcttc ctaacataat acaacgatcc ttcaaacgtt ttgcaatttc cctgacattg
      541 cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat cacaactaat
      601 tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag caatatgcct
      661 ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcagggga gatattaagt
      721 tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat tccatttggt
      781 acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa gtataaacta
      841 ggcactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt atgcatatgt
      901 ataaccactc ttgcctgagg agctcgctta cgaatttgag tgtatagctt catgctatta
      961 tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt caatattctc
     1021 tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt atatatgcga
     1081 ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt tgagtatcct
     1141 ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac ctgatacatc
     1201 cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt aaccgtaaat
     1261 attattttat caaccatctc gtatattaac ctttcataac attatattta attgctgtaa
     1321 aaacaccatc ga
//
LOCUS       DAZKGS010000431         1330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKGS010000431 DAZKGS010000000
VERSION     DAZKGS010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1209)
                     /locus_tag="ACHOQY_005279"
     CDS             complement(<1..1209)
                     /locus_tag="ACHOQY_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFP9681153.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTART"
BASE COUNT          403 a          273 c          239 g          415 t
ORIGIN      
        1 ggttcttgct gtattcggta cgcagcagac agcagcctcc tcatgagtag gggagtattt
       61 atatctggta ttaaactgat tactgtccgg atgtttcctt tcatttgctt cataacaatt
      121 atcagtctta cagtacatta tcgcgtgaag tatgggatgc tgcatgccaa aactagcatt
      181 atagaatagc cactcagcat catcggccag accaatatta gtggtcagtt ttattaacat
      241 aacataagtg ctaaatagtt catgaacaga acaatattca tagcctgtat aagtggcatt
      301 agctgtgcgt cctgtaatcc actcatcacc aatgggagcg ccagatggtg taagataata
      361 tttcaactgg gataaaaaaa tatctaatgc agtttgatac tgcggccgtt ggctcgctgc
      421 gatggctaat gccctaatat gctcatacgt atgtacgcca tgacctttaa gaaggtaacc
      481 agggtctatg acatttttat attgcagatc ttgctccgac acgcatcctg cggaataatc
      541 ttcatacagc cacaagcaat atgagagata ttcaatgtta ttggttattt cataaaggcg
      601 gtagaaaaca tctgttattg ttaatccgtg gctatgtcca gcaaaaccct gttccacaac
      661 gaaaggatgt gacgtttctt tagggtagcc agccataatg cattgtgcgg cttgggtaag
      721 agaagttaat aaactctcat tacgggtgga ctcataacat ccaagcacaa aacgcaaggc
      781 agtagcttgt gcccagaact ctccattttc tgctttacag ttaaatttta atgatgaatc
      841 ataaattcca atatagccat catcatcttg cgtatcgata agttccaaac ataaatcgct
      901 cgctttttcc tgccattctt tattatctaa caagtaggca ctgcggcaat atccatcctt
      961 ccaattcgat tgagtttcac tatcccacca ataatattgc atcatgttat catcgggatt
     1021 attctgtttt ttaataacgc catcttcagc agatacagtg cgtggagtta tacgatctct
     1081 tccatatatt ttatgctccg taataagatg gggaactaat ttatccagtt ctcctatatg
     1141 accgttcata taccattcca tcttattctt taaccaacca gtgggcttta tatcgttaaa
     1201 aaaaatcata aatatacctt tgcataaaaa agtttagacg ttataaaaga caaatagatt
     1261 gtgatcgcct acagacgatc acaatgaaag gaaaatatta acggtttttg ctatcactca
     1321 aatgtttttc
//
LOCUS       DAZKGS010000432         1327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKGS010000432 DAZKGS010000000
VERSION     DAZKGS010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..275
                     /locus_tag="ACHOQY_005280"
     CDS             <1..275
                     /locus_tag="ACHOQY_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9681154.1"
                     /translation="ARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSH
                     SSEYYREIVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            298..1260
                     /locus_tag="ACHOQY_005281"
     CDS             298..1260
                     /locus_tag="ACHOQY_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9681155.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
BASE COUNT          339 a          297 c          400 g          291 t
ORIGIN      
        1 tggcccgtaa tacccagaaa attttccgtt ggctggataa agacacccct gatgctgttg
       61 aaaaaatgca ggctctgtta ccggcgatcg aaaaggcgat gccgcctttg ctggtggccc
      121 gtatgcgcag ccacagttct gaatattacc gtgagatcgt cgaacggagg gatcggctgg
      181 tgaaggatgt cgatgatttt gttgcgtcag cggttgtttt gtatgaccag atgaatcgcg
      241 gcggcccggc agggaatgct gtggtgatgc actaaaagca cggtgttcgg gggttttatg
      301 agcagcaagc ttcatggtct tgtctgggaa gggtgcgcct tcaccggcat gatcttatcc
      361 agggtggcgg ttatggcccg tcttgcagac tacagcaatg acgagggcgt gtcatggcct
      421 gccattgaaa ctatccggcg tcagatcggt gcaagaagtg aatccacagt gaaatcggct
      481 attgcagaac tggcgaaaga gggctggctg acgaaggaag agcgtaaggt cggtgggcgt
      541 aatgtaagca atatctatcg gcttaatgtg gaaaaactcg aagcagctgc ggcggcggcg
      601 cgtgagtcat ataaaccgaa aagaaaaatt agcccggcaa aaaatgaccc gttaacagtt
      661 gacccgtcaa atattgcccc ctcaacggtt gacccgtcaa attttgatgg atcaactgtt
      721 gataacaaac tgccgattag gggggcgatg attgaccccg atccgtcagt attaaaacct
      781 gatccgtcag ataaaagatc ttcttgtccg gacgcttcgc aaccggaccc gcagacggct
      841 gaacaggatt ttttaacccg acaccctgac gcggttgtgt tcagtgcgaa aaaacgccag
      901 tggggaagtc aggaagattt ggtgtgcgca cagtggatct ggggacgaat cgtgagtctt
      961 tacgagcagg cggccagcga tgatggcgag atcacgagac cgaaagaacc caactggact
     1021 gcatgggcca atgacgtgcg gacaatgcgg atgctggatg gcagaactca cagacaaatt
     1081 tgtgaaatgt ttgggcgtct ccagcgggat tcgttctggg taaaaaacat catgagtccg
     1141 gcaaaactcc gggaaaaatg ggatgaactg gttatccgcc tggggcgttc gcccgcgcag
     1201 cgttgcgtga atcacatttc tgaaccggac actgaaatac cgccgggatt cagggggtga
     1261 cgtgtcatga aaaacattgc ggcagttggg gttcttgaac gtattcgcag acttgcacca
     1321 caggggg
//
LOCUS       DAZKGS010000433         1320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKGS010000433 DAZKGS010000000
VERSION     DAZKGS010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..456
                     /locus_tag="ACHOQY_005282"
     CDS             <1..456
                     /locus_tag="ACHOQY_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9681156.1"
                     /translation="LVDRWRELEETAVNIPKTLPEALRLAADLAEQKMQLENQLAIAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            456..746
                     /locus_tag="ACHOQY_005283"
     CDS             456..746
                     /locus_tag="ACHOQY_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9681157.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            743..1105
                     /locus_tag="ACHOQY_005284"
     CDS             743..1105
                     /locus_tag="ACHOQY_005284"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9681158.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1102..1290
                     /locus_tag="ACHOQY_005285"
     CDS             1102..1290
                     /locus_tag="ACHOQY_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFP9681159.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
BASE COUNT          395 a          317 c          339 g          269 t
ORIGIN      
        1 cttgttgacc gttggcgaga gcttgaagaa actgcggtta atatccccaa aacgttaccg
       61 gaagcgttgc gccttgctgc tgatcttgct gagcagaaaa tgcaactgga aaaccagctc
      121 gcaattgccg cacctaaagt tgagtttgcc gatcgcgttg gcgaggccag cggaattttg
      181 attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc gtggatgcgc
      241 gatcacaaaa tccttattgc ttcaggttcc cggcgcaatg tgccaatgca ggaatatatg
      301 gatcgcggct atttcacagt gaaagaaaca gcggtcaaca caaatcacgg aatacagata
      361 tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgaccagaaa gctgctcgat
      421 aacggaatgc tgaaagtaac aggggaggct gcttaatggc taacctacgc aaagaatctc
      481 gcggcagaga atgccaggta cgtatttacg gcatatgcaa tggcaatcct gaaactacag
      541 ttctggcaca ttaccggatg gctggaattt gcggaacggg aatgaagcct gacgacctga
      601 tcggcgcatg ggcttgtagc gcgtgtcacg atgaaatcga ccgacgcacc cataacctcg
      661 acaacaaaga cgccagactt taccacctcg aaggcgtgat caggacgcag gcgatactgc
      721 tgaaggaggg gaagattaag tcatgaacga atatcagttt gtgcttccat acccgccgtc
      781 ggtgaatacc tactggcgaa gacggggaag ccaatactac atcagcgata aaggccagaa
      841 ataccgaaaa gacgttcagc aaatcatcca ccaactcaag ttagatattt tcaccaaatc
      901 acgactccgc atcaaagtaa tcgcagacgt tccagactcc cgccgccgcg acctagataa
      961 catcctgaag ggtttactcg actcccttat ccatgccgga tttgcggaag acgacgagca
     1021 attcgatgac attcgcgtaa ttcgtggtgt gaaagtacca ggcggacggc ttggaataaa
     1081 aatcaccgaa ctggagaacg catgaacgcc acaattcaaa cgataccaga gcttcttatc
     1141 cagacacgag gcaatcagac cgaagtggcg aggatgcttt cctgcgcaag aggaacagtg
     1201 ctcaagtaca accgagacag caaaggcgag cgtcatgtaa tagttaacgg cgtcctgatg
     1261 gtcaaacagg gcaagagggg aagacgatga gactcgaaag cgtagctaaa tttcattcgc
//
LOCUS       DAZKGS010000434         1313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKGS010000434 DAZKGS010000000
VERSION     DAZKGS010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            299..592
                     /gene="iss"
                     /locus_tag="ACHOQY_005286"
     CDS             299..592
                     /gene="iss"
                     /locus_tag="ACHOQY_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP9681160.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHOQY_005287"
     CDS             complement(683..865)
                     /gene="rzoD"
                     /locus_tag="ACHOQY_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP9681161.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1082..>1313)
                     /gene="rrrD"
                     /locus_tag="ACHOQY_005288"
     CDS             complement(1082..>1313)
                     /gene="rrrD"
                     /locus_tag="ACHOQY_005288"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9681162.1"
                     /translation="IEVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACD
                     QLRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          333 a          317 c          304 g          359 t
ORIGIN      
        1 tcagcacagt aaaacccggt gtgcatcgtt tttgattatt cccgcacact cacgcagaag
       61 gaattccccg tcgggctacg gtcatggtta atgcgggaat acggcgacga tacagcgcag
      121 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
      181 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      241 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      301 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      361 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      421 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      481 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      541 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      601 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      661 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      721 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      781 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      841 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      901 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      961 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
     1021 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
     1081 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1141 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1201 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1261 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttcg atg
//
LOCUS       DAZKGS010000435         1310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKGS010000435 DAZKGS010000000
VERSION     DAZKGS010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            259..468
                     /locus_tag="ACHOQY_005289"
     CDS             259..468
                     /locus_tag="ACHOQY_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681163.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            585..>1310
                     /locus_tag="ACHOQY_005290"
     CDS             585..>1310
                     /locus_tag="ACHOQY_005290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9681164.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVS"
BASE COUNT          389 a          241 c          306 g          374 t
ORIGIN      
        1 tttttattgc aactatcaat gattgtgatg tcgaagagtt atttgttaaa caaaatcgtc
       61 acctcaaagt gatcaatgtc atgaaaataa ggtgaaaaat gataatgccg acttatttat
      121 catttatata ttgtcgctgt ttatctattt aaattttttg tgcttttgtt tttgtcttta
      181 ccctccgttt tggtaatctg aacgcatagg ttatttgacc aggaatgttt gttaatccaa
      241 tgaatggatg gttaatctat gaaactgaaa aaactctccg ggtttagttt gggacttatt
      301 gctctggcgg tgggtaatgc atatgctgaa cagtttattc agcacgatga atataaacat
      361 gctgtggcaa taaaagatgg aaaagtaaaa gatattgata atgcgctagt tataacaacg
      421 ggtaatggaa gttacgggta tccgttgcag gagagggtag caagttaacc attaacaatg
      481 gcgtcattgt aacaacgggc ggtttatatc cttctgacaa tagtcctcta agcacagcat
      541 taacagctag cgcaatcgta agtgaatttg gcggtgctgt tattttgcag ggtaaaaaca
      601 cgattgtcat aacgggggat tactcaattg gtttgctaag tcaaacgagt ggaaacctga
      661 atactgacac cataataaga gtcaactctg acggttcggt taccccctct ttttctgacg
      721 gagatgatac atttattgtt actgcgggta atcatgcagt cggcgttctt gcgtgtgcat
      781 ctcccggaag cgcgcgtgcg tgtgtatctt ctcttgatga agaaagtact accgatacag
      841 gaagtaatga aaataatgcc atagcaaaac tggatatggc aaaaggggag atcacaaccc
      901 acggaacaga aagttatgcc gcgtacgcta atggcactgt cgtcaaggct ggtgacacgc
      961 ttgattatac caatgccagc gttacattaa ccgatgttga tatcaccacg catggggaca
     1021 atgctcatgc catcgctgcc cgccagggga cagtttcatt taaccaggga gaaatttata
     1081 caacgggtcc tgacgccgcg actgctaaaa tttataatgg cggcacggtg acgctgaaaa
     1141 atacatctgc agtcgcgcat caggggtctg gaattgtgct ggagtcttcc ataaatggtc
     1201 aggaagcaac ggtagatatt ttatctggta gctcactagg gtcagcaaat gaaatcctct
     1261 accataaaaa tgagacgagt aacgtgacca ttacggatag tgaggtatca
//
LOCUS       DAZKGS010000436         1299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKGS010000436 DAZKGS010000000
VERSION     DAZKGS010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..>1299
                     /gene="nleA"
                     /locus_tag="ACHOQY_005291"
     CDS             19..>1299
                     /gene="nleA"
                     /locus_tag="ACHOQY_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP9681165.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPL
                     KNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKT
                     ENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLD
                     NPNTKYAVLTNAAANALGAVGGFAVSRFTGTDPMLSPHIGAMVGQAAGHAIQYNTPGL
                     KPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGADI
                     RAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSN"
BASE COUNT          405 a          287 c          282 g          325 t
ORIGIN      
        1 atgcaataag gatttatcat gaacattcaa ccgatcgtaa catccggaat caccacacaa
       61 aacaatcgac atcatcacgc agaacaaacg tcccctacac aaataccgca atccgaatta
      121 cctaatggat gcgaaacggg atttgttgtt catatcccag aggatatgca gcgacatgca
      181 ccggaatgcg gtgaaacaac agctctactg agcttgataa aagatgaagg tctgctctct
      241 gggctggata aatatcttgc acctcatctt gaagaaggct ctgcaggaaa aaaagcattg
      301 gatatgtttg gtttattcaa tgtctctcag atggcattag aaatacccag caccgttccg
      361 ggtatctctg gtaaatatgg tgtccagcta aacattgtaa aaccagatat tcatcctaca
      421 tcaggtaatt attttttaca gatattccct ttgcatgatg aaataggtat taattttaaa
      481 gaccttcctg gtccattaaa aaatgcatta agcaacagca atataccaac cactgtatcg
      541 actgctgcat ccactattgc atcagccact acttcgacgg taaccaccgc gtcaaaagac
      601 ccaataccat ggtttggatt aacagctcaa gtagttcgta atcatggtgt ggaacttcct
      661 atagtcaaaa ctgaaaatgg atggaagctt gttggagaaa ctcctcttac tcctgatggc
      721 cccaaagcaa attatactga agagtgggtg atcagaccgg gagaagcaga ttttaaatat
      781 ggtgcatctc cactacaggc aactctaggg ctggagtttg gcgcacattt caagtgggat
      841 ttagataacc ctaatactaa atatgccgtt cttaccaatg ctgccgcaaa tgcgcttggt
      901 gctgtagggg gatttgcagt atccagattt actggtacag atccaatgtt aagtcctcat
      961 atcggtgcaa tggttgggca agcagcgggg catgccatac agtataatac ccccggatta
     1021 aagccagaca ctattttatg gtgggcaggt acgactctgg ggctggcaga tttaaacaaa
     1081 gccgagacag gtgaaggcag attcactgac tatcctcgta tatggtggca cgcaagagaa
     1141 ggtgccatct tccctaataa agaagatatt gcccgtgcaa caggggctga tatacgagct
     1201 atggaagaag gtatacccgt tggacagcga catccaaatc cagaggatgt ggtcatcgat
     1261 atcgaaagca atggctcacc acatcataac ccatcaaat
//
LOCUS       DAZKGS010000437         1282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKGS010000437 DAZKGS010000000
VERSION     DAZKGS010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1236
                     /gene="ygeH"
                     /locus_tag="ACHOQY_005292"
     CDS             <1..1236
                     /gene="ygeH"
                     /locus_tag="ACHOQY_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP9681166.1"
                     /translation="GHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCI
                     ETIYRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKK
                     IDGLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLF
                     LIASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKE
                     LYDFTPESIYRAMTIFDILQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKA
                     LELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALV
                     HFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESH
                     RVIIDNILKLKQLTRICMR"
BASE COUNT          459 a          225 c          217 g          381 t
ORIGIN      
        1 ggtcatgttg tactgaaaga tatgatcatc gaatcagtat ggaaaaatat tattgttagt
       61 gacgagtccc tgacaagatg tatctattct ttgcgctgca tttttgaaaa aattggctat
      121 gatcgttgca tagaaacaat ctaccggaaa ggttatcgtt tcagcgggca ggttttcaaa
      181 actaaaatta atgaagataa tacttcagac tattccatag ctatattccc tttcactact
      241 tcgttgaata cactggatcc attaatactt aatcaggaat tagtgcaaat catttcaaat
      301 aaaaaaatcg atggtctcta tacctatccg atggctgcga caaatttttg taatgatcac
      361 atatctcaaa attcattctt gagcagattc aagccagatt atttcgttac aggaagaata
      421 aaccagaata atgcagtgaa cactttatac attgagttga tcgacgctaa aaaccttttc
      481 ctcatcgcca gtaatcatct ccctgttgat gaactacata atacatcaca atttattata
      541 gataatatcc ttcaaacggt acataaacca gaacgatctg taagattagc taagcaggac
      601 caaggatata agaatcatta tttatcagat gaaatgttag ccggaaagaa agaactttac
      661 gacttcaccc ctgaaagcat ttacagggcc atgactatat ttgatatatt acaaaataaa
      721 agtgatatac agacgctaaa aacagaatgt tattgccttc tagcggaatg ccatatgtct
      781 ttggcacttc atggaaaaag tgaacttgaa cttgctgctc aaaaagcatt agagctttta
      841 gattatgtat cagacataac cactgtcgat ggaaaaattt tagctattat gggactgata
      901 actggtctgt ctggacaagc aaaagtatct catatcttat ttgaacaggc taagatacac
      961 ccaactgata tagcctctct ctactactat agggcactag tccactttca taatgaaaaa
     1021 attgaagagg caaggatttg tatagacaaa tcactacaac tcgaacccag aagacgaaaa
     1081 gcagttgtga taaaagaatg tgtagatatg tatgtgccta acccgctcaa aaacaacatt
     1141 aaactctact ataaagaaac tgagagtgaa agccatcgag ttataattga caacattttg
     1201 aaattaaagc agctgacgag aatttgtatg cgataaattt ttattgacgc aattttatta
     1261 tttagatgta tgcaacttgt ta
//
LOCUS       DAZKGS010000438         1280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKGS010000438 DAZKGS010000000
VERSION     DAZKGS010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..140)
                     /locus_tag="ACHOQY_005293"
     CDS             complement(<1..140)
                     /locus_tag="ACHOQY_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681167.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IAT"
     gene            complement(133..366)
                     /locus_tag="ACHOQY_005294"
     CDS             complement(133..366)
                     /locus_tag="ACHOQY_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681168.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEAHYRRKFPEDVSHA"
     gene            complement(359..598)
                     /locus_tag="ACHOQY_005295"
     CDS             complement(359..598)
                     /locus_tag="ACHOQY_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681169.1"
                     /translation="MCNNTRPDAAAEAIITLMHALIDISVIADRAHKHDTSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGYA"
     gene            complement(588..794)
                     /locus_tag="ACHOQY_005296"
     CDS             complement(588..794)
                     /locus_tag="ACHOQY_005296"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9681170.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQEVCHVQ"
     gene            991..>1280
                     /locus_tag="ACHOQY_005297"
     CDS             991..>1280
                     /locus_tag="ACHOQY_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9681171.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKT"
BASE COUNT          326 a          311 c          309 g          334 t
ORIGIN      
        1 gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc aagccatatg
       61 cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg gtatattgtt
      121 tcgtggtaaa tatcacgcat ggcttacatc ctcaggaaat tttcttctgt aatgcgcctc
      181 tgccacatat tccgcataat cggcggcgat attcagtaca tcaagccccg ttgatttata
      241 ttctctcgtg gaaagtaaga aaaaagccgc tctaataagc tctggcattg acgaaagcgc
      301 atcagccgca tcatcaggaa cgccggaaaa ttcctgtttc agggaattaa aacgatcatc
      361 acgcataacc acccccattt tcacaatcag caatcaggat ggctttagcc tcattcagcg
      421 ccatatcagc actaagttgc ataaccgcca gcgaatgagg aacgaaagcc ccggcatatt
      481 ctgtttcact ggtgtcgtgc ttatgcgccc tgtcagcaat aacagaaata tcaatcagcg
      541 catgcattag cgttatgatg gcttcggcgg ctgcgtccgg acgggtgtta ttgcacatgg
      601 cacacctcct gacgaatacg ggcagcgaat accatcacgc agccagccgg ggattgcagg
      661 cgtgcttcct gttcgctggt ggcctcaatg gtaatcacgc gcggttgtgc agtgctcagg
      721 gcgataaaac gccaaatgta tttattcagg ttgtgcgagt cccgcccttg cgggtgtgtg
      781 gtatgatttc tcatagctac ctcgatactc tcgttatcgt tggtggttag aagccccgtt
      841 actgctccta cagtgcgggg tttcgtcgtt tctgcacctt gcattaacaa ggtgtaagta
      901 actttattta taggtggctt acatgtcaat gcttttatgt aagacttttt atgtgtatat
      961 tgtcttacac tttcattgag aggattacag atggctacag gttcaaaaaa cgcaaaatca
     1021 caatcactga cagctcggat cccgcatgat gttattgaag gcatggaatc cgtaaaactg
     1081 gacggtgaaa gcaacgccgg attcatagta accgccatgc gcggtgaaat cgcccgccgc
     1141 caggcagaag gaagcggaga aaacctcctg atttcttcgc tcaatgcact ggcgcaggtg
     1201 gaaaaactcg gaatcaaagc cgccgaggag atcgggcagc tcgtcactgt cgcacgtgaa
     1261 gaactccagc gccgcaagac
//
LOCUS       DAZKGS010000439         1275 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKGS010000439 DAZKGS010000000
VERSION     DAZKGS010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(378..1232)
                     /gene="rclR"
                     /locus_tag="ACHOQY_005298"
     CDS             complement(378..1232)
                     /gene="rclR"
                     /locus_tag="ACHOQY_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9681172.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          364 a          310 c          273 g          328 t
ORIGIN      
        1 cttcaaatgc aataactaat tttatttctt tcggcatcgc tcacatttta tggggaaatc
       61 gacgttcatt tttggcataa gaaatccatt taaaaaggct acatccctta tacctattca
      121 gagctactta ttcaggggtt attgagatat acactgatgg tgtacaaaaa aatcaccgac
      181 aataatcacg tatcaagtaa tagtcggggt tttatttaat tgaagcaatt tagtttgcat
      241 ttttatttaa aaactgagct agagtccatc agaaaaaaat atataaagcc caaaccaccg
      301 ttggaaatag aaatagttga aaggctcaca actcccggaa tgcggattat ctttgccctg
      361 atttctggcg gttttattca gggtgcaagc tgtctgaccc tttcccgata ttctcccgga
      421 gtacaaccaa actcgcggac aaacgccttg tgaaaagatg attcactggc atagcctact
      481 gactcagcga tcaccacaac agggagcatt tcccgggaaa acatctgggc cgctatttgt
      541 agacgcaact ttgttaatac agccagcggc gtggttccgg aaacatcacg gaaaagctgg
      601 gcaaaacttg cccgggacat gtgagcgatg ctggccagcg attcgacggt ccaggcgtgt
      661 cctggcattt ccagcatttg ctgtattacc gcaccaagac gtggatgcag aagcaaactg
      721 agaatgtttt tctcagtatt aacctgtgca atccactcac gcaccgcgag ggtaaagaat
      781 gtagcgcaga tctggctaca cagtgcatcc actcccggca tcgccgatct ggattcctgt
      841 tgtaaaaacg ggatcgcctc cctcagccag ttatattcca cgctgtggtt aaccggtgct
      901 aaaaacagcg tttccggcaa agacgttaaa aaataacgcg ccgaatgttg caaccgaaga
      961 gtgccgcaga caatacaggt cgattcatta tcgacatgac tcaggcgatg agctgaattt
     1021 tgtggtagca ggaccacatt tcccgggcgt aatgtaaaaa tctctcccgt cggcatttcc
     1081 agcttcgccg ctccttgcgt taacgcatgc caacgaataa ccgataattc cccggcacca
     1141 tgtggaagct gccagtcact tcctaacacg caattcttat cgatcgttcc ttgtggagcg
     1201 ttaagcatca acaaacggct aagggcatcc atatcagact cctgagtggt ttttgattac
     1261 aaacaccaac aatct
//
LOCUS       DAZKGS010000440         1261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKGS010000440 DAZKGS010000000
VERSION     DAZKGS010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1261)
                     /locus_tag="ACHOQY_005299"
                     /pseudo
     CDS             complement(1..1261)
                     /locus_tag="ACHOQY_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          251 a          392 c          310 g          308 t
ORIGIN      
        1 ttgtgcctgc gcggccatct tcccctcaaa tgtgcgctgt aaatccgcca gcaccgtgga
       61 gaattcttcg cccagtgcac gaataatgga cagttcccgt tccgtcattt tctcagtatc
      121 cccctgaaca tcgctttcac tgcatcatgc tctgtttcac tgattgcctt attaccgtca
      181 gatgcgccgt caggcagttg tgctgaaact gttttcccgg ccgacgcgaa cgggtcctca
      241 cgggcatcac gacgggacag cgcctccaga ctgtagttct gctgctgaag atacagtgca
      301 tcaccgccgg caaggggcgg caggttctca cgtttacggg cctcattggg cgtgagaagc
      361 gtatttttca ccgattcacc cagtgttttc atgcgccgtt cgctgtccat tctcagcagc
      421 gtggtgacgt caaactccgt gctctcgttt tcccccgttt ccagcgcctc atccagtaac
      481 agctcaatgg actcaatcag cgtctgcaga cactgggaat aatactgctg ctccagcgcc
      541 tccacgttgt cactggaagg cgggtggcca acgccaatct tgtaggccgg gacacggaac
      601 accgaacaga caatttcagc cgtcattttc agttgttcca ccgtctgcgc atccaccggt
      661 gaaaacgtcg tggggctgta ttttgccccg ttgctcagta tggccgtctt cccggcattt
      721 tcgcccgtat atccgctgtc ccagttcccc ttcagttttt tcgcgttttc ttccgtaata
      781 ctgccgggga cctcaatcac gcctgatggt cgcccgccat ttctgaaaaa atacgtcgaa
      841 tttgcctgaa tatgatgccc ctgcatggcg gccagtcctg cggcatacac cggcggcagc
      901 cccacaagcg gatgaaaaaa acagttaaaa cggtcgtgga tcacctcccg ggcaggcacc
      961 gtcaccgact ctgtgatccc acagttccgg tccggcgtga tgcggtagaa cacgtcgccg
     1021 tcatccgcca ccagaggttc aacccggttc cagtccagaa tacgcagttc tttgatctgc
     1081 ccccgggggt tgcggatttt cagcaccacc gtattgccgt gacgcaattt ggaattcagc
     1141 cacagttcaa aaaactggat gcgattctgc tgcgcattag gacgacgaca gagacgggca
     1201 gtatctccct gccgcttttc ccgacgtatt ccctgtacat cagtctgcat caggcgaagc
     1261 c
//
LOCUS       DAZKGS010000441         1244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKGS010000441 DAZKGS010000000
VERSION     DAZKGS010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..209
                     /gene="dicB"
                     /locus_tag="ACHOQY_005300"
     CDS             21..209
                     /gene="dicB"
                     /locus_tag="ACHOQY_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9681173.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            206..394
                     /locus_tag="ACHOQY_005301"
     CDS             206..394
                     /locus_tag="ACHOQY_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9681174.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            490..>1244
                     /locus_tag="ACHOQY_005302"
     CDS             490..>1244
                     /locus_tag="ACHOQY_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9681175.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          376 a          281 c          290 g          297 t
ORIGIN      
        1 tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg tctgaaggtt
       61 gttttgatat tggtgttctg ctcagtaacc gggagtttac ggaagatgcc attaagatga
      121 gaaaatatga accttatctt ctcaatgata attccatact ttccagaatt gcccttcttg
      181 aacttggtat tatcggagaa cagcagtgac ttcagcattt gcactggtga tgaccgtttt
      241 tcttataacg ggtgagccac aaaatgtgat taccggaatt tatgacagta agtcatcctg
      301 cattcaggta agggacgaac aaaaaatccc cggtgaatgc ctcccgttaa aaaaagtatc
      361 gctgaacctg aataacgaaa taccggctgg ataacccgcc agccatatta acgccatacc
      421 aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct gtaatgaggt
      481 taaaacaaaa tgagtaaagt ctttatttgc gccgccattc cggacgaaca ggcaataaag
      541 gaagaaggtg ccgtcgctgt agccactgcc attgaagccg gtgatgaacg tcgcgcccgc
      601 gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg cgcttataaa
      661 tttcttgtct gcgaggataa acccggtata ccccgccctg ccctcgattc ctgggatgct
      721 gaatatatgc aggaaaaccg ctgggatgag gaatccgctt cctttattcc ggtcgaacca
      781 gaatccgatc cgatgaacgt caattttgac aagctgtccc ttgaagtaca gaacgcggtc
      841 ctggttaagt tcggtacatg tgaaaacatc accgttgata tggcgattga cgcgcaggaa
      901 ttactgcagg aagacgttgc tacctttgac gggcatatcg ttgaagcact gatgaaaacg
      961 cctgaaatta acgctatgta tccggaacgc aaactgttcg ctatcggatg ggttaaacac
     1021 aaatgtaatc cgggtgccaa atggcccgaa attcaggctg aattacgtaa ctggaaaaaa
     1081 cggcaggacg cagagcgcaa agagactgga aaatacacgt ctgttgttga tctcgccagc
     1141 gccagagtca atcaacagaa cactgaaaac tcagcaggaa aaatcaaccc agtcactgcc
     1201 gccatttgtc gcgaatacaa gcagacatgg aaaacgctgg atga
//
LOCUS       DAZKGS010000442         1243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKGS010000442 DAZKGS010000000
VERSION     DAZKGS010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1243
                     /locus_tag="ACHOQY_005303"
     CDS             <1..>1243
                     /locus_tag="ACHOQY_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP9681176.1"
                     /translation="ENNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDMMPAY
                     SKQVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVNNQNIDEVNYNEISDADKNIIK
                     KILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVI
                     DAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSK
                     FRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTT
                     GTGKYDWLNPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSKLKTENLPELFLTKI
                     DTDTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMND
                     DIAKRSLFSLQVQIAEKIRRMT"
BASE COUNT          452 a          207 c          241 g          343 t
ORIGIN      
        1 gaaaataatc acaattatca aactgaaatg ttgcttcgat ggaactatcc tgccgcctca
       61 gacctgctca ggatgtatat ccttaaagag catggtggta tttatacaga cacagacatg
      121 atgcctgcat actctaaaca agtaattttt aaaattatga tgcagacaaa cggagataat
      181 cgtttcttgg aagatttgaa actacgtcgt gcaatatctg atggtgtatt aagatatgtt
      241 aataaccaaa atattgatga agttaactat aatgaaatca gtgatgcaga taaaaacatt
      301 atcaagaaga tattaacaga aatatctaaa atgccagaag atagcatttt tactaagatc
      361 aatacaagga ttcctcgaga cacaatgccc atccttcgtc gttatcacct atggcctgat
      421 ggatggaata ttcgtgggct caatggattc atgctatcac ataaaggtag tgaagtgatt
      481 gatgctgtta tcgcaggcca gaatcaggct tacagggaac taagaagaat aagagataat
      541 attcatagtg aaatatactt caaacaaact gatgaattgt cctcacttcc agatacagac
      601 aaaattggag ggattctggt aaaaaaatac ctttcaggaa gtcttttttc aaaattcaga
      661 caagatacta ttatcccgga ggcattgagc accctccaga tatcaggtcc tgacttgatt
      721 caaagaaaga tgttgcaatt tttcaggagt agaggggtgt taggtgaaga gttcattaat
      781 gaaagaaaac tgagtgataa agcttatatt ggtgtctaca aaacaactgg cacagggaaa
      841 tatgactggt taaaccctga atcgatcggc gttaatgatg tcacgcccgc agatgaaagt
      901 acctggtgta taggaaaagg ccggtgtgtt gatgacttcc tgttcaaaga tgtttcaaaa
      961 ctaaaaacag aaaatcttcc agaattattc ttaacaaaaa tagatactga tacgtttttc
     1021 tctcagtggt caaccaaaac caagaaagat ctgcaaaaaa aaatacaaga ccttactgta
     1081 cgttataatg agctaattga ctcgtcgact atcgacttta aaaatctata tgaaatagat
     1141 caaatgctcc atatgattat gctagagatg aatgatgata tagccaaaag gtcattgttt
     1201 tcattgcaag ttcaaatagc cgaaaaaatt cggaggatga cca
//
LOCUS       DAZKGS010000443         1243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKGS010000443 DAZKGS010000000
VERSION     DAZKGS010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..77
                     /locus_tag="ACHOQY_005304"
     CDS             <1..77
                     /locus_tag="ACHOQY_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9681177.1"
                     /translation="RISQDISTRDEGGGGTVVVVGRQG"
     gene            420..>1243
                     /locus_tag="ACHOQY_005305"
     CDS             420..>1243
                     /locus_tag="ACHOQY_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681178.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRSFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAW"
BASE COUNT          328 a          297 c          375 g          243 t
ORIGIN      
        1 ggcgaatctc ccaggacatc agcacccgtg atgaaggcgg gggcggaacg gtcgtggttg
       61 tcgggcgaca gggataaaac ataaaaaaat cccgcagtga tcgcggagct gcggggacag
      121 acaaatgaag atcaatgtta aggagttgtt tttgttactc gggcaaaaaa acactaacgc
      181 agcgaaatta taagcgccac agtcagtgtg tgaaaatgtg aagatattca gaatttttat
      241 gccattaccg gttttaacca acaggattat cggtgggcat gaaagaaaac cccggtatct
      301 gctgataccg gggtttctct ttagcatggc agaaatgtgt ttcatgcttt tcgggcgaag
      361 gatatccgac ttctgtacgg aatggcaagt ggcggttaat ttattcaggg gaaggctgta
      421 tgggaaaagg tggcggtaag gcacacacgc ctcgtgaggc gaaggataat ctcaaatcca
      481 cgcagatgat gagtgtgatt gatgcgattg gtgagggacc gatagaaggt ccggtgaagg
      541 gactgcagag tattctggtg aacaaaaccc cactgacgga cacggacggc aatcccgtga
      601 tacacggtgt gaccgcggtc tggcgtgccg gggagcagga gcagacacca ccggaaggct
      661 ttgagtcctc cggagctgaa accggactgg gcgtggaagt gacgaaggca aaaccggtga
      721 cgcgcaccat tacgtccgcg aacattgacc gcctgcgggt taccttcggg gtgcagtcac
      781 tggtggagac cacctcaaag ggtgaccgta acccggcatc cgtccgcctg ctgattcagt
      841 tacagcgtaa cggtaactgg gtgacagaaa aggacgtcac cattaacggc aagaccacct
      901 cacagttcct ggcctcggtg attctggata atctgcctcc ccggtccttt aacatccgga
      961 tggtcaggga gacggcggac agcaccacgg accagctgca gaataagacg ctctggtcgt
     1021 catacaccga aatcatcgat gtgaaacagt gctacccgaa cacggcgatt gtggggctgc
     1081 aggtggatgc ggagcagttc ggcggccagc agatgacggt gaactaccat atccgcggtc
     1141 gcatcatcca ggtgccgtca aactatgacc cggaaaaacg cacgtacagt ggtatctggg
     1201 acggcagtct gaaaccggca tacagcaaca acccggcctg gtg
//
LOCUS       DAZKGS010000444         1241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKGS010000444 DAZKGS010000000
VERSION     DAZKGS010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..634
                     /gene="rfaY"
                     /locus_tag="ACHOQY_005306"
     CDS             <1..634
                     /gene="rfaY"
                     /locus_tag="ACHOQY_005306"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFP9681179.1"
                     /translation="NDFLTCRVKTLKVFRSIDDTKVILIDTARGPLVLKVYAPKHKMT
                     ERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLNFAHYYIMLIEYIEG
                     VGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTA
                     VLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVKLGLKSK"
     gene            659..>1241
                     /locus_tag="ACHOQY_005307"
     CDS             659..>1241
                     /locus_tag="ACHOQY_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9681180.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQ"
BASE COUNT          439 a          167 c          230 g          405 t
ORIGIN      
        1 aaatgacttt ttaacttgca gggttaaaac attaaaagtt tttcgttcga ttgatgatac
       61 taaagtcatt cttattgaca ctgctcgtgg acctcttgta cttaaagttt atgcgccaaa
      121 gcataaaatg accgaacgtt tcttaaaatc ttgcataaaa aaagattatt atgaaaattt
      181 aatttatcaa accgatcggg tacgtggtga aggcatccaa tcaattaatg actatttttt
      241 gctagctgaa cgtaaaacat taaatttcgc tcattattat attatgctca ttgagtatat
      301 tgaaggtgtc gggttaaatg agtatttgga aatttctgaa gatctgaagg atcaattatc
      361 agaatcgata aaagagttgc atcagcatgg aatggtgtct ggcgatcctc ataaaggaaa
      421 ttttatagta tccgaaaaag gtttaagact aattgacctt tcaggtaaaa aaacaaccgc
      481 tgttttaaaa gcaaaagatc gcattgatct tgaacggcac tataatataa aaaatgaatt
      541 gaaagacttt ggctatacat atcttatatt caaaaagaaa attaaaaaag taatcaggga
      601 tgtaaaagtc aaactaggat tgaagagcaa ataatgatta tgatgaaggg taattaaatt
      661 ggattttaaa catcttactc aatttaaaga tataattgaa ctggacaagc gccccgttaa
      721 acttgatgaa cgggaaacgt ttaatgtctc atggggtatt gatgagaact accaggttgg
      781 ggctgcgatt tcaattgctt caattcttga aaataataaa caaaacaaat ttacctttca
      841 cataatcgct gattacttag acaaagagta tattgaatta ttatcacaat tagcaacgaa
      901 gtatcaaaca gtaattaaat tatatcatat tgattctgag ccattgaagg cgctacctca
      961 atcaaatatc tggccagtat ctatttatta tcgtttgctt tcatttgatt atttttctgc
     1021 gcgattggat tcattattat atcttgatgc tgatatcgtc tgtaagggtt cattgaacga
     1081 gttaatagca ttagagttta aagatgaata tggggcagtg gtaattgatg tagatgctat
     1141 gcaaagtaaa agcgctgagc gtttgtgtaa tgaggatttt aacggtagct attttaactc
     1201 tggtgtaatg tatattaatt tacgggaatg gttaaaacaa a
//
LOCUS       DAZKGS010000445         1241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKGS010000445 DAZKGS010000000
VERSION     DAZKGS010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..1153
                     /locus_tag="ACHOQY_005308"
     CDS             173..1153
                     /locus_tag="ACHOQY_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFP9681181.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          305 a          304 c          369 g          263 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag
      901 cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt
      961 gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg
     1021 tctggcggct aatggcaaga aaggaaaggt gattctcggc gcgatgatgc gcaagctggc
     1081 acaggtggcg tatggagtgc tgaagtcagg cgtgccgttc gatgcgtcac ggcataatcc
     1141 ggtagcggcg taaaaatcgc ggaagggatg aaaaaaacag cgcctgacgg cgctgtgtct
     1201 ggcatgcctg caatccggga aaccggacca ggaaaaaact t
//
LOCUS       DAZKGS010000446         1238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKGS010000446 DAZKGS010000000
VERSION     DAZKGS010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(220..936)
                     /locus_tag="ACHOQY_005309"
     CDS             complement(220..936)
                     /locus_tag="ACHOQY_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9681182.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            986..1201
                     /locus_tag="ACHOQY_005310"
     CDS             986..1201
                     /locus_tag="ACHOQY_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9681183.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          337 a          247 c          279 g          375 t
ORIGIN      
        1 agttattaaa gttgaaccct gccgtcatgt tcttacgcct cgggctggct acttaacccc
       61 tgatcactgc cgggtaactc gaagtatttc cctgcgttct gtggggcggg gtgggttggt
      121 atgttgttaa ggtaacaaga gttacctttc gagtcaatac aatgttgcaa aaggtacatt
      181 tgagggcata aaaaacccgc aatgaatgcg ggttctgact cagtctaagt attgatgtat
      241 ttgtgaaact ttacctttaa tggtgtaacc accattcagt tcgatgggtt tgtaaagcgg
      301 attcagtgac aacagataga tgtttggtcc gtcaatcgca acttttttta gtgttacgtt
      361 tggcgttcct tccaattgga ttaagattat ttttcccacc agttctctaa tgttacttga
      421 gcatggtgtg atcagcacgg tagatccgtc ggggatggtt gggaggccgt tagagtttgt
      481 catcgcatct ccctcaacat gcaataaaaa agagttttca gcggtttttg tcatgacatc
      541 aacccagttc ttaataccag gaatcttggt tactggacaa ctcatatccc aataaccagc
      601 ctgttcccac gttaaaacgg gcaaccgggc gatgttgtca ctaatgtaag ggtactgatt
      661 cagacgcaga tcatcggttt tatcgtgacc gtcctttcca taaagaatcc attcaggaga
      721 tttggaaagc aattttgaca gtagatacaa attctcaccg tcaggttttg aagagccatt
      781 ttcccatttt gttacggata cacgagatat gccgattgct ttcgcaacct gctgttgggt
      841 taatccaacg tcttttcgac gattccgaat acgttcgctg atagtgtttt tcatgtaacc
      901 aatgttacta ccaagtgatg ttgctatggt tgacattgtt atgtaactat tgttaccctt
      961 ctgctcgaaa taacaggaga gttttatgtt caaagatgat gttctgcgct atttcaaaaa
     1021 aaagcgacta gtagctgagg ctcttggaat ttcacatgtg gctgttgtgc ggtggaaagc
     1081 agttattccc aaacttcgcg caatggaact ggatgaaatt actaacggtg aattgaaata
     1141 caacccagaa ctttacaaga agcaggatag cacctcgaac gaaggaaaga atgattcatg
     1201 aaaatcaagc atgaacacat ccgcatggcg atgaatgt
//
LOCUS       DAZKGS010000447         1237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKGS010000447 DAZKGS010000000
VERSION     DAZKGS010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /locus_tag="ACHOQY_005311"
     CDS             <1..517
                     /locus_tag="ACHOQY_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9681184.1"
                     /translation="YGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGTGIWT
                     ICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTDPQKAGIASF
                     CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQ
                     ESALACWGIDR"
     gene            676..813
                     /locus_tag="ACHOQY_005312"
     CDS             676..813
                     /locus_tag="ACHOQY_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681185.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            815..1177
                     /locus_tag="ACHOQY_005313"
                     /pseudo
     CDS             815..1177
                     /locus_tag="ACHOQY_005313"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1149..1220
                     /locus_tag="ACHOQY_005314"
                     /pseudo
     CDS             1149..1220
                     /locus_tag="ACHOQY_005314"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
BASE COUNT          303 a          297 c          363 g          274 t
ORIGIN      
        1 atacggcctg tcggctgccg ttctggcgct gattggtgca ggggcgtctg cgcctgaaat
       61 cctcgaccag tttctggatg aaaaggaagg taaccacacc acagcatacc gtgatggtac
      121 gggtatctgg accatctgcc gcggtgccat cctggtggat ggtaaacctg tcgtcccggg
      181 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgagcgtg ataaggcgct
      241 ggcatgggtg gagaaaaaca tcaaagtgcc gctgaccgac ccccagaaag cggggatcgc
      301 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
      361 aattaatgct ggtgaccgca ggggagcatg cgaggcgatt cgctggtgga ttaaggacgg
      421 tggcagagac tgccgtattc gctcaaacaa ctgctacggt caggtctcac ggcgtgacca
      481 ggagagcgcg ctggcgtgct ggggaattga cagataagca gaatattttg ctgaaaaatg
      541 cggtttgctc acacgggcgg ataacacgaa atcctgcgaa ctggcaaaaa ctaagtgaat
      601 aaaagtaaaa ccccgtttgt tggccgcaag cggggttttg tgtttcctga ctccggaaaa
      661 gtcaaaggag aaagtgtgtt tgattttagc aaactgattc gggagattcg agtgatggct
      721 gaaaaattat ccacctggaa gttcatcctt atctggctgg tgtttgtgat tatggcctcc
      781 ggttatttca tcggtcagat acgctggtgg tgaaatgaac cgcgtactgt gcgtggtcat
      841 cattgccctg ctggtggcct gtggtgcgct tagtctgggg ctgaatcatt accgtgataa
      901 cgccataacc tacaaagagc agcgcgataa aaaagtcagt gagctggagc tggcaaatgc
      961 aaccattact gatatgcagc agcgccagcg tgatgttgct gcacttgatg ccagatactc
     1021 gagggaatta gccgatgcga gagctgaaaa tgaaactctt cgcgctgacg ttgccgctgg
     1081 tcaccgcagc ctgcggatca acgccacctg tccaggtccc gtgcgtgaag ccaccggcac
     1141 cgcccgcgtg gataatgcaa ccggccccca actggcagac accgttacac gggattattt
     1201 caccctcaga gagcggctga tgacgatgca gaagcaa
//
LOCUS       DAZKGS010000448         1217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKGS010000448 DAZKGS010000000
VERSION     DAZKGS010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            688..1188
                     /locus_tag="ACHOQY_005315"
                     /pseudo
     CDS             688..1188
                     /locus_tag="ACHOQY_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          354 a          234 c          256 g          373 t
ORIGIN      
        1 agattattga caaaaccaaa tataaataac gactgcctcg gcatgccagt taacagctat
       61 ccgtatggcg tgttcggttt tgcgaataaa aaatggttac tgtagaagcg gatttccacc
      121 acatgtcaac catgaatggt cagtgatgcg agacgactgg gatgatttgg tcgatgactt
      181 cttccggcga atactgatag aaacattgaa atattgtaag agaaagcgtt caaaattctg
      241 tggcacgtga aaaattctac agcgtcatca ccttattcag tcgaccttca aaataaacgt
      301 ttaacagaga caatcaccat ttggattgtc cgctgaggag gcgaacaatt tcctagacag
      361 ctttatcatg tcagcccaca ttgttggcgc tggcaatact aaggacaaac gatggggaag
      421 tagcttttta gcgttatttt cgggagtaaa aaacgtggta tctttccgta tgtcagttgt
      481 atcttgctgg tattcgtagt cgtgataagg catacaacga tatttatctt gacgttagtg
      541 aagaataagt tgttttgtga tctatgttaa tctgggatta caatccgggc ttgatacatc
      601 gcatcacgac ttttattctc ttcagaatag aatatgttac tccccgcttt acaggctcag
      661 gatttgaatt acaaattata cttttaagtg aacaataacc aagggtattt cttatttcct
      721 gaaaccaggc ttggcccatt tcgggatgag tttgaaaaaa ctcttgacgc atacgatagt
      781 gatgaaataa acgaaacaaa ttacgtcaac aaactcaggc gtcttgcctg acatgctcct
      841 gacatcattg atattcacgc tcatctggca tatgcatttg cgctctttat gctgcaattc
      901 tggcgaatgt tcatttacag cgtcatcagg atgccgttat actcattgat aaaatactaa
      961 catataaccc tgagaataat cagggaacac gctggttact gggggcggaa ttgttacgtg
     1021 ctggcaagca tggcagggct ctgtacgtat tgacaggaca tgtcaatgaa tttcccccct
     1081 actggtacga gcttgctctt ttacatttta tgaatgaggc atatattaat gctgcaacgg
     1141 catttcgata tggattttta acaagtatat attgttgaaa tgctttgtta gaatctttat
     1201 ccattcccta gggctgt
//
LOCUS       DAZKGS010000449         1209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKGS010000449 DAZKGS010000000
VERSION     DAZKGS010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..791)
                     /locus_tag="ACHOQY_005316"
     CDS             complement(<1..791)
                     /locus_tag="ACHOQY_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9681186.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSG"
     gene            complement(772..>1209)
                     /locus_tag="ACHOQY_005317"
     CDS             complement(772..>1209)
                     /locus_tag="ACHOQY_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9681187.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          274 a          397 c          304 g          234 t
ORIGIN      
        1 tctccggaac gctgcagctg tgcaacatac gcaatctgct ctgccgtcac gttacggaac
       61 tggcgcgcca tcgccatcag tcccgacgtc gggtcagtgg tcagcttccc gaaggcttca
      121 gcgactttat ccacctccac accggatgca gacgcaaaac gcgcgacact ctggttgatg
      181 gcatcaaact gttcaccacc acgcacaccg gcattcacca gggctgccag tgactctctc
      241 gcctggttaa acgtcagccc tgctgcctgc ccggctcttg agagagtcag catacgatcg
      301 gcagtcagtc cggactgatt accggaaaga accagggttt tattaaacgc tgaaagcgtg
      361 gaatccccct ggtaccaggc gtacgccagc gcacctgtcg ccaccgccag cgaggtgacc
      421 ccgaccatcg gcagggtgat cgcaccggca agccccctga gcatggggat catcccgccg
      481 aaggagtcct tcacctgacc gccctgttgc agcaggatca gccagggatt ctgaccaccg
      541 gcaagctgcg tggcgatatc cgtaaactgt gcgggcaggg tgcgcatggc cgctttatac
      601 tgtccgacgg aaatcccggc tttttgcgca gccagcgcct gacggctcag gccctgttca
      661 acagcagtgg cggtttttct ggcgtcagta tccagtcctg aaaaatgacg ccttacccgg
      721 ctcatctgct catcgaaacg gaccgcatcc agactcaggt caataataag atcaccaacc
      781 ggctgggaca tatctcacac ctcccggaat ccccgctgaa gccatcatta atgcggcatc
      841 atccaccatg acatccgcca catccgcaga cgataaaata tcgccccctc cgtccccacc
      901 gaaccggacg cctccggcaa gtcctgccgc tttctgcatc agcattttgt cctcatccag
      961 cctctccacc tgctcttcct catgccgggg gacaagcaga ctgaaatcag agggatgcat
     1021 atccggatcg caaaaaaaca ggctgagtac agcgtacatc agcccggaaa aatgcatatc
     1081 cagttgggta tcctgaaaat aatgcgtgcg gtaaaaatgt cgccagtcgg catattcggt
     1141 ggatgtcatc ccggcaagca tggcgcgcca gtcaggcctc cccatctctc gcgccagtct
     1201 gagggcaaa
//
LOCUS       DAZKGS010000450         1203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKGS010000450 DAZKGS010000000
VERSION     DAZKGS010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..258)
                     /locus_tag="ACHOQY_005318"
     CDS             complement(<1..258)
                     /locus_tag="ACHOQY_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFP9681188.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEINSNRDLLGMFFLSLKEDSVLL"
     gene            509..1120
                     /locus_tag="ACHOQY_005319"
     CDS             509..1120
                     /locus_tag="ACHOQY_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFP9681189.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          401 a          202 c          253 g          347 t
ORIGIN      
        1 caaaagtacg ctatcttctt ttaatgatag aaaaaacata ccgagtagat ctcgattgct
       61 atttatttct agtattttat caatttttga agattcatta ttgaataaat aacaataaaa
      121 aaacaggacc tcagattgag gtgcacactc cagtatccat tcatgaccat cgtgatcatg
      181 gaaagatagg gcgttagttt gttcttcaag agttattccg aacgtatgtg caagatctgt
      241 gcgaaattgt tcattcatat tgtagtcctg tcattctttc aatgagggta tgcttaaacg
      301 taattaactc gtgagctctg gcacaaataa taagacgaaa atataagatg caagtaatag
      361 tgttgcatat ttatgatagc atgtgataaa tgtagtgcaa ctacttgtag atagtgccgg
      421 aggaaaattt aaatataaaa caattattgt ttttaatgaa aataaaaaac attttgttat
      481 cgttatataa tatataaagg tttattgtat gtttagtcca atgacaatgg taggtagatc
      541 gttagctcag gcggctacac aaactcttag acctgcggtc accagagctg cgacacaagc
      601 gggggtggga gccagtggca tgaagttcgt gcccatgcag tcgaacttta tgattaatca
      661 tggcaaactg actaaccagc tacttcaggc cgtggccaaa caaacccgta gcggtgatac
      721 tcagcaatgg ttcaaacagg agcaaattac ttatatatcc agaacagtaa acagagctat
      781 tgatgactat tgcatgagta ataattcggt aattggtaag gaaaccaaaa gccacatttt
      841 caaggctgtt gaaagcgcga taaagcagcc tctcgatatg aattgtgcac aaacgtcgat
      901 tggccatttc ttacagtcca acaaatactt taataaaaaa gttgatgagc agtgtggcaa
      961 aggggtggat ccaattacac gctttaacac ccagaccagg atgatagaac aagtctcaca
     1021 agaaatcttt gaacagaaat ttggtggttt gaaagttagt gatatcaaag ctctgactca
     1081 gagcacgatt gcacagcatg tacaggatac tcggttgtag gaataacctg ttatatatgc
     1141 gtgttgggca taagttaagc ttcctggtgt atagcgtagt ctttgctgat gtaagaaaag
     1201 gct
//
LOCUS       DAZKGS010000451         1198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKGS010000451 DAZKGS010000000
VERSION     DAZKGS010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1198)
                     /locus_tag="ACHOQY_005320"
     CDS             complement(<1..>1198)
                     /locus_tag="ACHOQY_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9681190.1"
                     /translation="KWDTPKVVKGVSFMLRLTVVADDGSERLVSTARTTETTYRFTQL
                     APGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYDPT
                     VQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSA
                     FVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNE
                     ITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENT
                     PAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPP
                     AFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVGK
                     AFPRDSR"
BASE COUNT          248 a          356 c          286 g          308 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa cggtgttcac actgcggata taaaagtaat aatcatggcc cggtttgata
      781 ttgatactgg cggctatcca gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc
      841 tgcctgatat cggtaatccg cgtttccgaa aaccagaact caaactgtac cgtcgggtca
      901 tacaccgcaa gacgcgggac cgctgttatc tgaaaatagc ccggtgtcag ctcaatctgt
      961 gacggcgctg ccggtgcggc aatccggaac gataccgacg ccggatcgcc ctgctgcccc
     1021 cacgcattta ccgcccggac tgtcagcctg tagttccccg gcgccagttg cgtgaagcgg
     1081 tatgtggttt ccgtcgtccg ggccgtgctg accagccgct cactgccgtc atccgcgacc
     1141 acggtcaggc gaagcataaa gctcacgccc ttcaccacct tcggcgtgtc ccatttcg
//
LOCUS       DAZKGS010000452         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKGS010000452 DAZKGS010000000
VERSION     DAZKGS010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1193)
                     /locus_tag="ACHOQY_005321"
     CDS             complement(<1..>1193)
                     /locus_tag="ACHOQY_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9681191.1"
                     /translation="DPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTV
                     DFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLDREVTLPETGTSA
                     VNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRE
                     NTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLA
                     QAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLPLGEYTLTVRAIN
                     SYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIA
                     DIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAE
                     GYLDF"
BASE COUNT          231 a          371 c          344 g          247 t
ORIGIN      
        1 aaaatccagg taaccttccg catcatcgct cgcccgaccg acagcctcca cgaatgccga
       61 tttgccaaca gtattcacac tgcggatata aaaataataa tcatggcccg gtttgatatt
      121 gatactggca gctatccagt acagcgccga gccaagatag cgggctgcgg tttcaacctg
      181 cctgatatcc gcaatccgct tttccgagaa ccagaactca aactgtaccg tcgggtcata
      241 aacggcaaga tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga
      301 cggtgctgcc ggtgcggcaa tccggaaggt ggtggtggca ggttcaccct gctggccata
      361 gctgtttatc gcccgcaccg tcagggtgta ttccccgagc ggcaggccgc tgaaacggtg
      421 ctccgtgtct gcggtgatgg cggtggtcac cagtctggca tccgttccct taccactggt
      481 caggcgcaga ctgaagcgca cgcccttcac cacccgcggc gtgtcccatt tagcctgcgc
      541 cagatactgg ccgtcagctg cactcacctc caccgtcagg tgctgtactg ccggtgggat
      601 gacgctgttc agggaacctg actgcggctc aaagcgggca ccgttatcca cgatggcttc
      661 tttttccggt acgtgctgca ccgccgtgat ggcaaaggtg ccgtccgtgt tttcccggac
      721 ggagacacag cggaacaggc gacggcgcag tgacggcagg gagagtcccc acaccccgta
      781 tgtctccaca ccatcaggca gggtactgac ctgtatccgg tccggcgcgg ggtgtgcggt
      841 gatgtccaca ctcaccggct taccgctgcc gttaatcagg ttcaccgccg atgtacctgt
      901 ctccggcagg gtaacctcac ggtccagcgt cagggtgcgg gtggcagcat caatggacag
      961 gacacgtccg ccggtcaggg tcccggcata gtcattatca cagatttcaa taatgtcacc
     1021 gggtgtgtgc cgcagcccct gagacccgag cgtgaaatcc accgtctgcg tttccagcag
     1081 ttcggtcttt atcacccaca gtccggcacg gtgggcctga ccgcggctgg tacagccgaa
     1141 cgcgtccatc ttcagcaggt tgcgtccgta gcgcagtatg gcttccgggt ctt
//
LOCUS       DAZKGS010000453         1179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKGS010000453 DAZKGS010000000
VERSION     DAZKGS010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1115)
                     /locus_tag="ACHOQY_005322"
     CDS             complement(<1..1115)
                     /locus_tag="ACHOQY_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9681192.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGQIDNVATAVGALVAVGGARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIA"
BASE COUNT          240 a          400 c          286 g          253 t
ORIGIN      
        1 gcaatctgca tctccgtccc gcgggctgcc gccacggcct gctgagcacg gtacacggct
       61 gctcttgccc gcgccgtggc aatctgcgtt cccctgaact gtgcttccgc cagtgcaact
      121 tcattacgtg cagccgtcac aagtcctgcc gtggcagaca tcgctccgga ggccatattg
      181 ccaaagtacc gggcaccccc gacggcaacc agtgccccca cggctgttgc cacattatca
      241 atctgtccgg caacaccgtt cagcacgccg gagagcgttt tcgtcacccc gctggcctca
      301 ttcgcaccgc ccacccaggc cataaaggcg ttttccacct tcgtgatact actggaaacc
      361 gtttccggca tggcggcata ttcatcacgc aataccccca gctggctgat taacgcagga
      421 acgactttat ccgccgtcag tttgccgtcg tccgccatcg ccttaaggtc tttacgggcc
      481 acgcccatac ccgcagccag tgcacgtacg atccggtctc cgctttcatt gaccgaatta
      541 aattcctcac cgcgtaacac accctgtgcc agcgcctggc tgaactgggt gatcaccgag
      601 cccgcctctg ccgtactggc accggagatt ttcagccccg tggaaatggc ctccgtcacc
      661 ttcagcacat catcagcact gtaaccatat tcacgcattg aggctgccga gcgggcaaac
      721 agggccgcat tatctgaaaa tgccgtgccc gtccgctggc tgatatccat cagcactttc
      781 tgtgatgacg aaaattcatc agatgactgc gacgcctgtt tcagtcgggc attcacggaa
      841 ctccactcat ccgccagtga aatcaggtgt ccggtggcaa aggcaccggc aaatgcgcca
      901 gccattccga cagcagaacc gcgaatttcc gtcaactggc tgttcagttc tgccagggca
      961 cgtcgctgct cccgggctgc cgcagcggcc tgacgcccgc cattctgcag ggtccggtaa
     1021 tattcactgc ccatacggga agcccgctgg atctccgact ggaatgactg cgaatttgcc
     1081 gaaattttga taatcagttc acgtaacgtc gccattcacc tttctccggg cgtaaaaaaa
     1141 ccgcctcagc ggttctcatc attcatgact gtgctgcaa
//
LOCUS       DAZKGS010000454         1177 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKGS010000454 DAZKGS010000000
VERSION     DAZKGS010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            388..495
                     /locus_tag="ACHOQY_005323"
     CDS             388..495
                     /locus_tag="ACHOQY_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9681193.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            871..978
                     /locus_tag="ACHOQY_005324"
     CDS             871..978
                     /locus_tag="ACHOQY_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9681194.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(983..>1177)
                     /locus_tag="ACHOQY_005325"
     CDS             complement(983..>1177)
                     /locus_tag="ACHOQY_005325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681195.1"
                     /translation="FRLAADSTTVFLFRNPSLTHSVLRIPQNIIRLQPLHFSYEKGRR
                     VSPLRPGPGANCHYGSLTPA"
BASE COUNT          308 a          271 c          358 g          240 t
ORIGIN      
        1 aagcggaagt agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
      241 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa
      361 cgttgtcatt acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      421 catgatttag cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg
      481 aacaagcgga agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
      541 gatcaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
      601 ggcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
      661 gaaaaacagt cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg
      721 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
      781 atgcacctga caacatcacg ggggccattc ttgaacctga acaactcaag aatagccgcg
      841 aaacgttgtc attacaacac aggcggctat atgacgctcg cagaactggg catggccttc
      901 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
      961 ctgaacaagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
     1021 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
     1081 gcaagcggat aatattctgc ggaatgcgca gcacagagtg agtaagtgat ggatttcgga
     1141 acagaaaaac agtcgtagaa tcagcggcaa gccggaa
//
LOCUS       DAZKGS010000455         1169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKGS010000455 DAZKGS010000000
VERSION     DAZKGS010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..627)
                     /gene="nleE"
                     /locus_tag="ACHOQY_005326"
     CDS             complement(<1..627)
                     /gene="nleE"
                     /locus_tag="ACHOQY_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFP9681196.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEI"
     gene            complement(676..>1169)
                     /gene="nleB"
                     /locus_tag="ACHOQY_005327"
     CDS             complement(676..>1169)
                     /gene="nleB"
                     /locus_tag="ACHOQY_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9681197.1"
                     /translation="EGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTETGKTGCHN
                     ISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALL
                     AGLDIMKNKVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTS
                     SSW"
BASE COUNT          356 a          244 c          176 g          393 t
ORIGIN      
        1 tatttcccca ggcatgtagc taaagatttc tcccatttca cgtgcgataa tcatttcaga
       61 ttttactgcc tttaactctg gtaactcata atttaattcc tgcatgatat ctctttttat
      121 attatataat tctataaatc tatttgcccg ttgttctcct agagggctgt catttctgta
      181 cacaacaact atgtataaaa aatcattatt ttctttaggg aagatcttac aacaaatgtc
      241 cggaagctca cataatgcat gtaaatcagg attgtataaa ctatctacag aaaactgccc
      301 cattgggcgt tttccggata taactgttag ccagcttcct tccggtgtac tggcttcatt
      361 tctggaaata ggttcaaacg cttctggaca tacagaatac atagtcttca tataagaaaa
      421 ccgtctggct ttctcgttaa caaattcttc ctgttttagt tcagctactc ctctggttat
      481 accggatata tacttcttat catatatatt gggtgattca ttaaagtaat catgtttaat
      541 ttccgggtaa atatttttca ccacatgttc agcatattta gtatttatag gggacacgcc
      601 ctgagtatta gtaacaggat taatcatctg aatctatacc taaagaacga tatttttcaa
      661 ccacaaccgg aagacttacc atgaactgct ggtatacata ctggtattcg gtataatatt
      721 ttcatgctta aattcaataa aatcacaaaa agcattataa tcgtgtaacg atgaatagtt
      781 aaaatgccgc ttgataccct ttcctagacc atcataatat ggatgagcgt caactttatt
      841 cttcataata tcgaggcctg caagtaatgc tggatgatta ctacgattga caactatcgc
      901 accattttca agacttttta tctcatcatt acaatctaca tgcacagcga taccatcagg
      961 agcatacagg actcctaatt tatcggtaat aatcatgtcg gcatcaagat atatacatcc
     1021 gctacagggc gatatgttat ggcatccagt ttttcccgtc tctgtaaaca aagttccagc
     1081 cttcaacatg gccaaattcc tgaaaaaatc aaaccaagta tgttctcttt tttctgcata
     1141 tgactcaatc aaagagtcct taccttctg
//
LOCUS       DAZKGS010000456         1155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKGS010000456 DAZKGS010000000
VERSION     DAZKGS010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..865
                     /locus_tag="ACHOQY_005328"
     CDS             <1..865
                     /locus_tag="ACHOQY_005328"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9681198.1"
                     /translation="IKPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHS
                     NYGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYT
                     GLSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKE
                     TQSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEA
                     HGREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPAS
                     DAVNDLLNTPV"
     gene            875..>1155
                     /locus_tag="ACHOQY_005329"
     CDS             875..>1155
                     /locus_tag="ACHOQY_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9681199.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          256 a          339 c          356 g          204 t
ORIGIN      
        1 cataaaaccg gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc
       61 cagtgccgcc tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat
      121 gatggctcac agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat
      181 ttacagcggc agccataagg tggatggcaa cccctacagc catcttccgg atgacgtccg
      241 ggagacactg cagtaccgga tggacgcaac ccgccagatg tttgcgcaga aggtgtcggc
      301 atataccggc ctgtccgtgc aggctgtgct ggataccgag gctgcagtgt acagcggtca
      361 ggaggccatt gatgccggac tggctgatga acttgttaac agcaccgatg cgatcaccgt
      421 catgcgtgat gcactggatg cacgtaaatc ccgtctctca ggagggcgaa tgaccaaaga
      481 gactcaatca acaactgttt cagccactgc ttcgcaggct gacgttactg acgtggtgcc
      541 agcgacggag ggcgaaaacg ccagcgcggc gcagccggac gtgaacgcgc agatcaccgc
      601 agcggttgcg gcagaaaaca gccgcattat ggggatcctc aactgtgagg aggctcacgg
      661 acgcgaagaa caggcacgcg tgctggcaga aacccccggt atgaccgtgg aaacggcccg
      721 ccgcattctg gccgcagcac cacagagtgc acaggcgcgc agtgacactg cgctggatcg
      781 tctgatgcag ggtgccccgg caccgctggc tgcaggtaac ccggcatctg atgccgttaa
      841 cgatttgctg aacacaccag tgtaagggat gtttatgacg agcaaagaaa cctttaccca
      901 ttaccagccg ctgggcaaca gtgacccggc tcataccgca accgcgcccg gcggattgag
      961 tgcgaaagcg cctgcaatga ccccgctgat gctggacacc gccacccgta agctggttgc
     1021 gtgggatggc accaccgacg gtgctgccgt tggcattctg gcggttgctg ctgaccagac
     1081 cagcaccacg ctgacgttct acaagtccgg cacgttccgt tatgaggatg tgctctggcc
     1141 ggaggctgcc agcga
//
LOCUS       DAZKGS010000457         1154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKGS010000457 DAZKGS010000000
VERSION     DAZKGS010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..1062
                     /locus_tag="ACHOQY_005330"
                     /pseudo
     CDS             106..1062
                     /locus_tag="ACHOQY_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          314 a          239 c          280 g          321 t
ORIGIN      
        1 tcatagcaga ctggccggtc aaccgggtca gcgaactgct tccgtggcgc atagcactgc
       61 cagctgaata acacatcccc gtcaatacgg ccctcgctgt acgcttaccg ttcagtgggt
      121 attatgtcag atgaagccgg tattatattt gatggatata ccctgtctga gttgcctttt
      181 attaacaaaa tgtgggatgg ctctgtattg tctgtggaca gaaagaatga acccgaacag
      241 atgattgaaa atgccagaat gacgttatcc ctcatggtgc aacccggtct ttttgaccga
      301 tatatggagc gaaaaggttc tgttgccagg gacagtggtt ttcttgcccg ttgtcttatc
      361 agtaaacctg caaccacgca ggggaaacgc tttattaacg gagctgtgat tccgggagga
      421 tcattaacgg catttcatga acgattaatg gaactggcca gaggcagtat tgaaaaatcc
      481 agtgaggatg aacggtattg tctgcatttt tcgcctgaag cgcaaaaaat atttattgag
      541 cactataatg ttctggagca ggatttatcc ccttccggac ccttaagccc gtttcgggga
      601 catgtttcaa aaaaaacaga aaacattgca agaattgcag cgttatttca gtatttcagc
      661 tatggtgaag ggaaaatctc tgccgatatc atgacgtctg ctgtcgtaat aagttcgtgg
      721 tacacagatg aatataaaaa attatttgcg ttacctgatg aatctgaact gcagcagaag
      781 gacgccgagg agctgttcga ctggctcatt gaggagtgcc gcggagaatg cccgccccgt
      841 gtcaggaaaa attatattct ccagtgtggc ccgggccggt tcagaaacag gaaaaaactg
      901 aacgcattgc tgaacattct tgaatctcag ttcaggcttt ctgtcgtgcc ggaaggaaaa
      961 accatgtatg ttctgcttcc ccagatagcc agtttaaagt tgtctgatgt ctctggtatt
     1021 tttacctcgg gttatcacta caataagctg agagcgaagt aaccggtgag cagtttttct
     1081 ccattatgct tcagacttag atgattgatt attatgggga tttcatcacc gatattcaga
     1141 tgtatgaata tcgg
//
LOCUS       DAZKGS010000458         1139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKGS010000458 DAZKGS010000000
VERSION     DAZKGS010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(64..969)
                     /locus_tag="ACHOQY_005331"
     CDS             complement(64..969)
                     /locus_tag="ACHOQY_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9681200.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
BASE COUNT          315 a          242 c          262 g          320 t
ORIGIN      
        1 atactgcatt aatacaacat agtgatataa accaagaatc aatgttttta atctgtactt
       61 aatttatcgg tgatgttgcc tgatggaacg aatcattgat tgtttggcgg tatccagatg
      121 gtttcgcaat gccgttattg catctaaatc gctacggcaa atcagggcgc tgagaatggt
      181 catatgctca tcaacagcaa tgatattgcg ctgcttgaga tcgctttcat cccattgata
      241 gtgaaaatga aagatcaccg agataatttc aagtgattga ttaaaaaaga tgttgtcagc
      301 agcggataac agcatggcat gaaaatcacg gtctaattga gaaaacatcc gaaagttgct
      361 gccaatgctg tcgcgcaatg tcctgtgacg ttcgagcatg gttttcgcct gcaaccagcg
      421 gggatcgtca tcgggaaggt tgagaaagtg ttgcagggaa tgtgtttcga gcatttcgcg
      481 tagctcaaac agctgctcgg cgtaggattg atcgaactgc ttcatgctcc actggccgcg
      541 cttttcgctt tgaataagat tataacgacc gaatttcaaa agatattctc gtactactac
      601 cggactgacg cctgccgctc gcgccagctg aagttcggaa aagctttctc ccgcgcgtaa
      661 ctggcgcagg ttaatcatgg tgaaaaatgc ctgctcgaaa agacggtttt gctctttcag
      721 cgaggccgtg atgcaatcaa atccatcgtt ctgttcgggt ttgcgggcga taacataatt
      781 tttccccacc agggtgagca cgccacatgc gcttaaatgc tggagtatgt ggcgtaccgt
      841 agtgcgactg atgttataca tttctgccag cccactttgt gaaggcagcg gagaaggaat
      901 attgcccctc gccatatctt caatcacctg atttatgacg ttatgacgta aattctgcga
      961 acgactcatt cttccctcca gtccggcatt aaaaaccgat ttaaaacatt tttatgcgtg
     1021 atcatccgca tattttatgg gtaaattcag gtgctcttat aaattcgacg atatctgaaa
     1081 gtcataaaat aaaaggagcg gtgaatcatg tccacaatga aagtattaat ttgtcagga
//
LOCUS       DAZKGS010000459         1136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKGS010000459 DAZKGS010000000
VERSION     DAZKGS010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(515..1087)
                     /gene="hdeD"
                     /locus_tag="ACHOQY_005332"
     CDS             complement(515..1087)
                     /gene="hdeD"
                     /locus_tag="ACHOQY_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9681201.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          385 a          255 c          220 g          276 t
ORIGIN      
        1 tctgaagaat attactaaag ttaaaatgta atccgattta aatatcgagt ctccttgttt
       61 cgacttaagc tggcaattgg attgccagct ttcttttaac gcattgaaat tacctgagta
      121 aataacgcat tcatgtcttc aggtttttat atagcgtctg gagacacgga tatttatgca
      181 atgtaatcaa cgccagttgc aaaaaaatga acccggatta cttatttaac cgcccatagc
      241 atcaacgctt acggaactct ttgttcactt tcccacagca cttttaaaac attaaacctg
      301 actacggaaa atatcagcca tgataatgtt tgaaatggct aatttgccat ggagtgaaaa
      361 aaattagatg aaattcagta ggttgaaatg atcactagca ggtaattatt tcaatgatag
      421 tgcgcaattg atctacaaca ctgcgtagcg gagagagtat taatcggatc atagtcacat
      481 caagtgacta tgatcctggt gacaaccggg ataattattg ctgcttaacg aacaaactgg
      541 cgaagctgaa caggctggcg gcgctaaata tcagttcaat tcccaccagt gtggaaacca
      601 gcgttacaga caccatcggc gttgcaccaa ggaatatcca ggcaatgacg atatccagca
      661 caccaataac gagctgtagc cagctgcctt tcattgaacg ctgacgatac caactcatca
      721 ggcgaataac ccctgcaaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc
      781 ccagctccgg tgcgcggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga
      841 aaccggataa taccggccag aaattatgac tgcggttgct gaataacccg acaataagcg
      901 caatacccga gcagattaat aatgcaccca ctactgtgct taaaatatcg ccagagacga
      961 acgggaaact gatacacagc aacccgacga taaacagcag cacggcaata aactggattg
     1021 ctctgcgatg ctttttcagc atctccagat caaacttcaa aattgttgcc ttatctatat
     1081 ataacataga accaccctat aaaattaaga agaaaatccc ctgctatcaa tctatg
//
LOCUS       DAZKGS010000460         1132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKGS010000460 DAZKGS010000000
VERSION     DAZKGS010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            102..1076
                     /gene="cysB"
                     /locus_tag="ACHOQY_005333"
     CDS             102..1076
                     /gene="cysB"
                     /locus_tag="ACHOQY_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9681202.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          292 a          267 c          270 g          303 t
ORIGIN      
        1 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
       61 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
      121 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
      181 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
      241 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
      301 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
      361 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
      421 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
      481 accagggctc gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta
      541 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
      601 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
      661 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
      721 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg
      781 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
      841 cggtggatcc ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc
      901 acagtacaac caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt
      961 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
     1021 gctctaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
     1081 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tc
//
LOCUS       DAZKGS010000461         1129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKGS010000461 DAZKGS010000000
VERSION     DAZKGS010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..115)
                     /locus_tag="ACHOQY_005334"
     CDS             complement(<1..115)
                     /locus_tag="ACHOQY_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681203.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGH"
     gene            complement(99..>1129)
                     /locus_tag="ACHOQY_005335"
     CDS             complement(99..>1129)
                     /locus_tag="ACHOQY_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9681204.1"
                     /translation="PLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGL
                     LDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLA
                     LISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQ
                     GRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAAN
                     SITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGD
                     RQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQ
                     GSSDASNY"
BASE COUNT          271 a          326 c          223 g          309 t
ORIGIN      
        1 tatgtccata aaatacgatg tcagaatttg aattgttatc aaacccaagg taaacaacaa
       61 ataaacaaag ggtaattaaa aaaatgacaa gtttgcctct aataatttga agcatctgaa
      121 ctcccttgct ttgcttttat tggattatct gcttttatac catctaaccc gaagagttgc
      181 ccagtgttat atttttcaat acatgcatcg ctacatttgg cagaaaaatc gccacaagca
      241 ataccgacag tgttattttt aagatccatg attctttctt tagctggctg accttgtcta
      301 tcccctgccg cctcatgatt tttacctatc acgtcagcta ttgttgatcc aaatgtctta
      361 gtcattcgac acatcaagta acaatgtctc attgcatcgg cggccccatt atgagctccg
      421 ccaacaatag catccatatc ataaccgaac tggtaagcac tgttagtaat gctgtttgca
      481 gcaatcaatc caggtacacc agatacggca taaattagtc cacctgatgt catattctca
      541 atcgcatcta accccattgg gtctatcact tgtatgggat tcaacggata ctgatacata
      601 ttccatccac ctctcaaccc aatcgggtcc ggggtgatat accgcccctg caacggatcg
      661 tagtaccggt gacggttata gtacagcccc gactccttat catactgctg ccccggcagc
      721 cggtacggct ggtgcaggtg atgcgggttc tcctcattaa gctggttgcc ccattcatca
      781 tattccgcgc tccacgccgt attgccgtct tcactgataa gcgccagcgg caggccccgg
      841 tggtcacagt gataaagatg tatttttcgc tcaggcaaat aaaccggctc tatctgtgcc
      901 gccagccgct ccaccgtcag cccgcactgc gcaagccact ggcggctttc actgctcacg
      961 cagtttgccc ggatttcccc ctccagcctg tccagcagcc ccaccagttc agccgggaac
     1021 accacaccgt gaccgtcttc gctcccttcc tgctgtatct tctccgccag gctgcggcac
     1081 tgcgctttct cccgttcacc gttgtctgtt tcgatacgga tgagcggcg
//
LOCUS       DAZKGS010000462         1128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKGS010000462 DAZKGS010000000
VERSION     DAZKGS010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..1126)
                     /gene="ompC"
                     /locus_tag="ACHOQY_005336"
     CDS             complement(92..1126)
                     /gene="ompC"
                     /locus_tag="ACHOQY_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9681205.1"
                     /translation="MAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYAR
                     LGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRN
                     YGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQ
                     GKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQNAE
                     VWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEVVAQYQFDFGLRPSVAY
                     LQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATDD
                     IVAVGMVYQF"
BASE COUNT          319 a          251 c          242 g          316 t
ORIGIN      
        1 caaggttgtg cttctaaagg aagtggcttg agggagccac ttatatgttg gggaggcaaa
       61 gcctcccaca acatatgttt tagtaatcaa attagaactg gtaaaccata cctacagcaa
      121 cgatatcatc ggtagcaacg ccagatgctt tcgtgaaatc gctcttatca atcaggttga
      181 ttttgtagtc aacaaaagtg gacatatttt tgttgaagta ataggttgca cctacatcaa
      241 tatattcaac caggtcctga tcaccccaag cacccaagtc tttcccttta gattgcagat
      301 aagcaacgga cggacgcaga ccgaagtcga actgatattg tgcaactact tcgaagtttt
      361 gtgctttgtt ggcaatatgg tttttaccaa aaacagtcat gttttgggtt tcagaatagg
      421 tagtagccag atagatgttg ttcgcatcat atttcagacc agctgcccat acttcagcat
      481 tttgaccaga tgcattcagg ctgttgttac cgtagataac ctgattatta gtgcgatcag
      541 atttagcata ggttgcacct acgccgaaac cttcatactc ataagtagtg gagaaaccga
      601 aaccatcacc attagcttga gttacgtcag tacggtcatt tttaccctga tactgagcag
      661 caaagttcag accatcaacc agaccaaaga agtcattgtt acgataagtt gcaacacctg
      721 tggtgcgacc agtcatgaat acatctgttt gggtccaggt atcgccaccg aattctggca
      781 gaacgtcggt ccatgcacca atatcgtatg ctacaccgta gttacggcca taatcgatgg
      841 agccgtagtc accgaatttc aggccagcga aggcaagacg ggttttatct ttggaggaac
      901 cttgagattc agcgcggttg cctttgaatt catattccca ctgaccgaaa ccagtcagtt
      961 gatcgttgat ttgggtttca cctttgaagc caagacgggc ataagtagta tcaccatcat
     1021 ctgcatcatt agaggagaag tagtgcttag cattaacttt cccgtacaga tccagcttgt
     1081 tactgtcttt attataaatt tcagctgcct gagcagacat cgccatca
//
LOCUS       DAZKGS010000463         1127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKGS010000463 DAZKGS010000000
VERSION     DAZKGS010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..779
                     /locus_tag="ACHOQY_005337"
     CDS             <1..779
                     /locus_tag="ACHOQY_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9681206.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          308 c          302 g          270 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggtgtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZKGS010000464         1118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKGS010000464 DAZKGS010000000
VERSION     DAZKGS010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..790
                     /locus_tag="ACHOQY_005338"
     CDS             <1..790
                     /locus_tag="ACHOQY_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9681207.1"
                     /translation="RNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSENMTTMRHAP
                     GAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVVTMPELCWWL
                     IRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAKKVLALKVDP
                     ESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMATKAHDLF
                     VLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            808..>1118
                     /locus_tag="ACHOQY_005339"
     CDS             808..>1118
                     /locus_tag="ACHOQY_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9681208.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LG"
BASE COUNT          270 a          244 c          334 g          270 t
ORIGIN      
        1 ccgcaatgag gcagtgatcc gtcgtgctgg tggcgtggaa tgtcttgaaa gctggttact
       61 tcgtgaaaaa ggctgccagt ggcctcattc cgactggcac agcgagaaca tgaccacaat
      121 gcgacacgcg ccgggcgcaa tccgtctgtg ctggcactgc gataaccagc tgcgcgatca
      181 gttcacggaa cggctggaat caatggcaac ggataactgt gcccgctggg tgttgtctgt
      241 cgtgcgtcgg gatctcggtt ttgatgacag tcacgttgtg acaatgccgg aactgtgctg
      301 gtggctgatt cgtaatgatc tggcggatgc cttaccggaa agtgcagccc gtaaggcact
      361 gagattaccg aagcctgttg tgccgtctgt cacccgggaa agtgaccttg tgccttcggt
      421 tcctgccacc agcatcatcc aggataaagc gaaaaaggtg ctggcgctga aagtggatcc
      481 ggagtcgccg gagtctttta tgttacgccc aaaacgtcgc cgctgggtta atgaaaagta
      541 cacgcgatgg gttaagacgc agccgtgcgc atgttgtgga aaacctgctg atgatcccca
      601 ccacctgata ggtcacggtc agggtggaat ggctacaaaa gcgcatgacc tctttgtgtt
      661 gcctttgtgc agaaagcatc acgacgagct gcatgcggat accgtggcat ttgaagagaa
      721 gtatggctcc cagctggagc tgatatttcg ttttatcgat cgtgcactgg caattggcgt
      781 gctggcctga ttttgtggag aaagttgatg cgtgatatgt atgaagtatt ggaccgctgg
      841 ggtgcatggg ctgcagcaga taacagtggt gtggactggc agcctgttgc tgcagggttt
      901 aaaggtttac tacctcatgg aaagaaaaca cgccaacaat gcgatgatga tgaaggaatc
      961 atgattgaca gctgtgttgc gcgattgcgg aaatataagc ccgaagagta tgagttggtt
     1021 attgctcatt ttgttatcgg catctcacta agaactattg caaagaagca gaagtgttct
     1081 gatgggacaa taagaaaaga gttgcaaaca gctcttgg
//
LOCUS       DAZKGS010000465         1117 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKGS010000465 DAZKGS010000000
VERSION     DAZKGS010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..161
                     /locus_tag="ACHOQY_005340"
     CDS             <1..161
                     /locus_tag="ACHOQY_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9681209.1"
                     /translation="SLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELMSDPNSS
                     MYEEEYDG"
     gene            154..758
                     /locus_tag="ACHOQY_005341"
                     /pseudo
     CDS             154..758
                     /locus_tag="ACHOQY_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
     gene            755..961
                     /locus_tag="ACHOQY_005342"
     CDS             755..961
                     /locus_tag="ACHOQY_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9681210.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            939..>1117
                     /locus_tag="ACHOQY_005343"
     CDS             939..>1117
                     /locus_tag="ACHOQY_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9681211.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVE"
BASE COUNT          375 a          249 c          289 g          204 t
ORIGIN      
        1 aatcccttca ataccagaaa gaaagcgtcg agcgagcttt aacgtgcgct aactgcggtc
       61 agaagctgca tgtgctggaa gttcacgtgt gtgagcactg ctgtgcagaa ctgatgagcg
      121 atccgaatag ctcaatgtac gaggaagaat acgatggcta aaccagcgag aaggaaatgc
      181 aaaatatgca aggaatggtt tcacccggca ttctcaaatc agtggtggtg ctgcccggaa
      241 cacggaactc aattagcact cgaacgacga agtaaagaac gcgaaaaagc ggaaaaagca
      301 gcagagaaga aacgacgacg agaggagcag aaacagaaag ataaacttaa gattcgaaaa
      361 ctcgccttaa agccccgcag ttactggatt aaacaagccc aacaagctgt aaacgccttc
      421 atcagagaaa gagaccgcga cttaccatgt atctcgtgcg gaacgctcac gtctgctcag
      481 tgggatgccg gacattaccg gacaactgct gcggcacctc aactccgatt tgatgaacgc
      541 aatattcaca agcaatgcgt ggtgtgcaac cagcacaaaa gcggaaatct cgttccgtat
      601 cgcgtcgaac tgattaaccg catagggcag gaagcagtag acgaaatcga atcaaaccat
      661 agccgcatcg ctggactatc gaagagtgca aggcgatcaa ggcagagtac caacagaaac
      721 tcaaagacct gcgaaatagc agaagtgagg ccgcatgacg ttctcagtaa aaaccattcc
      781 agacatgctc gttgaagcat acggaaacca gacagaagta gcacgcagac tgaaatgtag
      841 tcgcggcacg gtaagaaaat acgttgatga taaagacggg aaaatgcacg ccatcgtcaa
      901 cgacgttctt atggttcatc gcggatggag tgaaagagat gcgctattac gaaagaattg
      961 atggcagcaa ataccgaaat atttgggtag ttggcgatct gcacggatgc tacacgaacc
     1021 tgatgaaaaa actggagacg ataggattcg acaccaaaaa agacctgctt atctcggttg
     1081 gcgatttggt cgatcgcggt acagagaacg tcgaatg
//
LOCUS       DAZKGS010000466         1106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKGS010000466 DAZKGS010000000
VERSION     DAZKGS010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..309
                     /locus_tag="ACHOQY_005344"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHOQY_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            401..891
                     /locus_tag="ACHOQY_005345"
                     /pseudo
     CDS             401..891
                     /locus_tag="ACHOQY_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            891..1040
                     /locus_tag="ACHOQY_005346"
     CDS             891..1040
                     /locus_tag="ACHOQY_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9681212.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
BASE COUNT          314 a          255 c          267 g          270 t
ORIGIN      
        1 aaaacagagt ttacctttcc tggtgcaaac agaagttatc tacaatcaag taacaccgat
       61 gttgctgccg cagcagctat aagtattgcg gcaacagaga tgaaaactat cctaccaaag
      121 gatttaacac cagaaaaata caataaaatt tatctgccag gggatggttc tgctggccta
      181 cctcttctta agtgtggaga tgaattttta tcaccaactg atatcgtcaa tcgtctggta
      241 gaacataatc ttcatgaagt agaagacatc agattaacat cctgtcactc agccaacata
      301 acaaaaaact agactggccc cctgaatctc cagacaacca gtatcactta aataagtgat
      361 agtcttaata ctagttttta gactagtcat tggagaacag atgattgatg tcttagggcc
      421 tgagaaacgc agacggcgta ccacacagga aaagatcgca attgttcagc agagctttga
      481 accggggatg acggtctccc tcgttgcccg gcaacatggt gtagcagcca gccagttatt
      541 tctctggcgt aagcaatacc aggaaggaag tcttactgct gtcgccgccg gagaacaggt
      601 tgttcctgcc tctgaacttg ctgccgccat gaagcagatt aaagaactcc agcgcctgct
      661 cggcaagaaa acgatggaaa atgaactcct caaagaagcc gttgaatatg gacgggcaaa
      721 aaagtggata gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt
      781 tgtctccggg tgtcgcgtgc gcagttgtac gtcattctca gacgaaccga tgactggatg
      841 gatggccgcc gcagtcgtca cactgatgat acggatgtgc ttctccgtat atcctcattg
      901 cgtggctgca ggacaatatc gactgcgaat cgggaattat ctttgacaac gatgaggata
      961 aaacggattc ggcagcactg ctgccctgta ttgaacaggc cagggaagcc aggtactttc
     1021 cttctgtgct ctcagcctga tcccgagtgt gattcccttt cgatatcaaa agggactgcg
     1081 ggtataattg agggtgcatt tttaat
//
LOCUS       DAZKGS010000467         1104 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKGS010000467 DAZKGS010000000
VERSION     DAZKGS010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..604)
                     /locus_tag="ACHOQY_005347"
     CDS             complement(59..604)
                     /locus_tag="ACHOQY_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9681213.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1001..>1104
                     /locus_tag="ACHOQY_005348"
     CDS             1001..>1104
                     /locus_tag="ACHOQY_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFP9681214.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGSG"
BASE COUNT          304 a          296 c          240 g          264 t
ORIGIN      
        1 ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc gcagcctgtt
       61 aacctgactg ttcgatatat tcactcagca accccggtat cagttcatcc agcgcggctg
      121 ctttgttcat ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg ttttccagtt
      181 ccggaaaacg ccgctgcacc gacaggggga tcccgtcgag aatactggca atttcacctg
      241 cgatccgcga cagcacgaaa gtacagaatg cggtttccac cacttcagcg gagtctctgg
      301 catttttcag ctcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt tcgtactcaa
      361 tagtccctgg ctggagatct gtctcgctgg cctgccgcag ttcttcaact tcccggcgca
      421 gcttttcgtt ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt
      481 cataaagcac ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc
      541 agttctgaat ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt
      601 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagcccgt
      661 tttcagcacc tgtcgtttcc tttcttttca gggggtgttt taaataaaaa cattaggtta
      721 cggcgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc gaaaacccgc
      781 gaggtcgccg ccccgtaacc tgtcggatcg ccggaaagga cccacgaaaa tgataataat
      841 tatcatctac atgaggttta tcacgacatg tgtgtacgcc atcaaaccac gagaaataat
      901 caattattac gcaggtatcg tattaattga tctgcatcaa attagcgtaa aagcaacttc
      961 agataataca aatcagcaac actgaatacg gggcaacatt atgtcatcaa agaacagaac
     1021 ccgcagaaca acaacccgca acatccgatt tccaaaccag ataattgaac aaattaacat
     1081 cgctcttgac ctgaaaggtt cagg
//
LOCUS       DAZKGS010000468         1096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKGS010000468 DAZKGS010000000
VERSION     DAZKGS010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1096
                     /locus_tag="ACHOQY_005349"
     CDS             <1..>1096
                     /locus_tag="ACHOQY_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
                     /protein_id="HFP9681215.1"
                     /translation="AIGRLYIKDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQ
                     NLSVINCEKLQITDIPSTVKNLHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRH
                     LEIRGYATDGIKNVPNGLLSLSINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEK
                     LPESLRSVTIHVEQKTTWNIVAEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAA
                     LHFRPGDIVYGLSSPREKLVNSIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSL
                     IKRALNEHERGTKFKQCLKNHNNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRT
                     VMFCADGLVNSLKLIANKSEGYGQSITASEL"
BASE COUNT          378 a          241 c          204 g          273 t
ORIGIN      
        1 gccattggtc gtttatatat caaagattgt gatattgagt atctgccaca gttaccaaac
       61 gaaataacat cagttacaat cgaaaattgc aataacctga ccacccttac aggattaccg
      121 gttaatacac aaaacctgtc cgtcattaac tgtgaaaaat tacaaatcac agacatacca
      181 tcgacagtaa aaaatctaca tattgaatta actgattcac catttataca catcatatct
      241 gaaggcatcg aatgcctgac ggtttgccac tgccatatat ctggagtgcc agagagtgtc
      301 cgtcatcttg agatcagagg ttacgccaca gacggcataa aaaatgttcc aaacgggtta
      361 ttatctctca gcatcaatcg ctacacccca gaaaatcagg ccagaattga cagcctgata
      421 tcaccgtcac tacagacgct atctctgact ggatgtagca atattatact gccggagaaa
      481 ctcccggaga gtctgagatc tgtaaccatt catgtggagc agaaaactac atggaacatc
      541 gtagctgaag gggttcctga tggactggat cttgatttac aaaatgtact actctctccg
      601 aatgtaatta aagaaaaaaa catcactttt cagggcaacg cactggatgc tgccttgcat
      661 tttcgtccgg gagacattgt ctatgggcta tcctctccca gagaaaaact tgtcaacagc
      721 atcaaactgg ttaacgactt ttccaaaaaa gatatcgtaa ttcagaatac attaacaaac
      781 gcagtatggg acgccagaac cccccgtaaa tataagcaag actcgcttat caaaagagca
      841 ttaaatgaac atgaaagagg aacaaaattt aaacagtgct taaaaaatca caataattat
      901 aatgtcatca tggccgacct ttccatatac aatcgagaca agttatgggc aaaaacaagc
      961 aaggcaggcc tagagtttca aacattaatt cgcaacagaa cggttatgtt ttgtgcggat
     1021 gggcttgtca attcactcaa actcatagct aacaagtcag agggctatgg tcagagtatt
     1081 accgccagcg aattac
//
LOCUS       DAZKGS010000469         1088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKGS010000469 DAZKGS010000000
VERSION     DAZKGS010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..364
                     /gene="rfbC"
                     /locus_tag="ACHOQY_005350"
     CDS             <1..364
                     /gene="rfbC"
                     /locus_tag="ACHOQY_005350"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFP9681216.1"
                     /translation="LHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLS
                     AENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNKPI
                     AISEKDKKGSSILRLNDY"
     gene            364..>1088
                     /gene="wzx"
                     /locus_tag="ACHOQY_005351"
     CDS             364..>1088
                     /gene="wzx"
                     /locus_tag="ACHOQY_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen flippase"
                     /protein_id="HFP9681217.1"
                     /translation="MLKKKLQKIKEYHSVLELAIIQGANAIFPVLVFPFFLITLGENI
                     FSSIAVGEVLALYVLIFSLYSFDIISVQKVISSVTKDEIFKVYILTLICRLCLFVISG
                     ICLLFITYLINKTLSVYLGLFLLYPVGMILQSNYFFQATNNNRPLAVFVLIARGMSLC
                     LIYFYNGPAGYLTSYYYVICVSGSYFLSGVLSLIYIYYQNKTNKAKIQWAEILEYICT
                     GYHLFIANIFVILYRNSNIIILGT"
BASE COUNT          328 a          136 c          215 g          409 t
ORIGIN      
        1 actgcactat cagctggaac cctatgctca aggtaaatta gttcgttgtg tggtcggtga
       61 agtatttgat gtagcagttg atattcgtaa atcgtcacct acatttggga aatgggttgg
      121 ggtgaatttg tctgctgaga ataagcgtca gttgtggatc cctgaggggt ttgcgcatgg
      181 ttttttggtg ttaagcgaga tagcagagtt tgtttataag acgacaaatt attatcatcc
      241 tgaatcagaa ggttgcataa aatgggatga ttcttttttg atgattgatt ggccaaataa
      301 accgatagca atctcagaaa aagataaaaa aggctcatca attttgaggc taaatgacta
      361 ttgatgttga aaaaaaaact tcaaaaaata aaggaatatc attcagtatt ggagttggca
      421 ataattcagg gtgcgaatgc catatttcct gtgttggtat tcccattttt tcttattacc
      481 ttaggggaaa acatcttttc aagtattgct gttggtgaag tactagcact atatgtgctt
      541 atattttcgc tatacagttt tgatattata agtgtgcaga aggtaatttc aagtgtgaca
      601 aaagatgaaa tatttaaagt ttacattctg acactaatct gtaggttgtg tttatttgtt
      661 atttcaggaa tatgtctttt atttataacg tatttaatta ataaaacatt aagtgtatac
      721 ttgggattgt ttttattgta cccagtaggg atgatattgc aatctaatta tttttttcag
      781 gctacgaata acaataggcc attggctgtt tttgtactaa ttgctcgtgg tatgtcatta
      841 tgtcttattt atttttataa tggaccagca ggctatttaa caagttatta ttatgtcatt
      901 tgtgtgtctg gttcgtattt tttatctggc gtgctatcgc ttatatatat atattatcaa
      961 aataagacta ataaagctaa aattcaatgg gcggaaattt tagaatatat atgcacaggt
     1021 tatcatctgt ttattgctaa tatatttgtt attctataca gaaatagtaa tattattatt
     1081 cttggcac
//
LOCUS       DAZKGS010000470         1087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKGS010000470 DAZKGS010000000
VERSION     DAZKGS010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHOQY_005352"
     CDS             <1..198
                     /locus_tag="ACHOQY_005352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681218.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>1087
                     /locus_tag="ACHOQY_005353"
     CDS             234..>1087
                     /locus_tag="ACHOQY_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681219.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAP"
BASE COUNT          350 a          179 c          225 g          333 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat cgaaataaga tctgttgaag cattctcaac aggctctata atattcgaag
     1021 atgaaaaaga agatgtctct tttaagtgtg aatttttcgt ttctcctatt gcacttaatg
     1081 ctccaag
//
LOCUS       DAZKGS010000471         1072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKGS010000471 DAZKGS010000000
VERSION     DAZKGS010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..1033
                     /locus_tag="ACHOQY_005354"
     CDS             74..1033
                     /locus_tag="ACHOQY_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFP9681220.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          366 a          205 c          207 g          294 t
ORIGIN      
        1 ttacgcacct tgtattacaa actcatgtat gtaaaatacg cgcaccattc aaaaaaaagg
       61 aagacaataa catatgaaaa aaagtgtcat cgctggcgtc tttattgctc tgtcatttac
      121 cacgtgttca gctatcgcga acagccttgc attatcatta gcaaatgatg atgcagggaa
      181 gtttcaacca atacttaatg atatttatgg caataaacat gaaaacagag atgattactc
      241 acaaggctta tttctgggat atagccacga tatctcagac tcgagccaat tatctctcca
      301 tattgcgcaa gatatttact ctccatcagg cagtaataaa agacacaaca cagctgtaac
      361 tggagacaga gcttttagtg catacactca cactggtatt gaatggaact cccttgcgaa
      421 tgactggatt cgctatcgat taggtactga cataggtgtt gttggccccg acgcaggcgg
      481 tcagaaagta caaaataaag ctcatgagat tattggggca gaaaaatatc atgcatggga
      541 tgatcaaata gagaatcgct acggttatac tgtaaaaggg atgctatcca tgacaccaag
      601 tatggatatt ttaggtgcta atgttggatt ataccctgaa gtttctgctg ttactggaaa
      661 cttatttcaa tatgtagcat atggcgcaac cattgccatt ggtaatgata aaaccttcaa
      721 ttcggataat ggctttggtc tgctggctcc ccgtggttta atgcatatgt ccgatacaag
      781 cggattcaaa tacaagattt ttgcaggtat ggaaagacga gatgtcaatc gcaactatac
      841 tctcgaagga aaaacaatac agacgaaaca aacaacagta tcgctaaaca aaactgttga
      901 tgaatatcaa gttggcgcaa caattgggta tgcacctgta gccttcacac tagcatttaa
      961 taaagtaaca tcagaattca agacagggga tgactattca tttataaatg gagcaatcac
     1021 cttctttttt taactgaatt gaattcaatc aaaataacat aagtccaaca aa
//
LOCUS       DAZKGS010000472         1064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKGS010000472 DAZKGS010000000
VERSION     DAZKGS010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..428
                     /locus_tag="ACHOQY_005355"
     CDS             57..428
                     /locus_tag="ACHOQY_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681221.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            449..640
                     /locus_tag="ACHOQY_005356"
     CDS             449..640
                     /locus_tag="ACHOQY_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFP9681222.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            642..920
                     /locus_tag="ACHOQY_005357"
     CDS             642..920
                     /locus_tag="ACHOQY_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9681223.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          343 a          232 c          239 g          250 t
ORIGIN      
        1 ataaaccatt aagtcaagta tttgcttgtt tagtgattac taaagataat taccacatgc
       61 aaaaaaaaga aattcgccgt ttacgtctca aggagtggtt taaagataaa actctgccac
      121 ccaaagagaa gagctaccta tctcaactaa tgagtgggag agcctcgttt ggagaaaagg
      181 ctgccagaag aatagagcaa acatacggga tgccggaagg gtatctggat gcggaatacg
      241 cagaacaacc gggggtttct ccaccacatg cagggttaac gtctaatcaa ctggaattat
      301 tgcagatttt ttcagccttc cctgaggatg agcaacgcca gataatcagc gagttaaagc
      361 agaaaaaaga atcaatggaa gatctcatag cgagatggat tgcggcgcaa aaatgccgcc
      421 gcgcctgagt tataaaaccg gaggaaacat gaatagagcc ctttcaccaa tggtttctga
      481 atttgaaacc attgaacaag aaaacagtta caacgaatgg ctgcgtgcga aagtagcaac
      541 gagccttgca gatccgcgcc cagcaattcc ccatgacgaa gttgagcgca gaatggcaga
      601 acgctttgct aagatgcgca aggaacggag caagcagtaa aatgttaccc gtgttatggc
      661 ttgaaagcgc agataccgac ctagatgata taactagtta tattgctcgt ttcgacatag
      721 atgcggctga acgcttatgg cagcgattaa ggggttgtgt gctgccgtta tccgaacatc
      781 cgtatttata cccaccaagc gacagagtac ctggcttgcg tgagattgta gcccacccta
      841 actatataat tctataccgc gtaacaacat caagcgttga agtagtaaac gtgatccacg
      901 caagacgcca gtttccctaa ctttcactac caatagaaac ataacaaccg caacgacttt
      961 atcaaaagcg ttgtgtttgt tatgtcccgc ggtttagttt ttacttgact taagtttaat
     1021 gtttattaaa ctaaaaatac caccccgccc cacagaacgc aggg
//
LOCUS       DAZKGS010000473         1053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKGS010000473 DAZKGS010000000
VERSION     DAZKGS010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..844
                     /locus_tag="ACHOQY_005358"
     CDS             <1..844
                     /locus_tag="ACHOQY_005358"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9681224.1"
                     /translation="SKAELTADLMWLSANRTGEESAEQLNYSGCDLSGLSLAGLNFAS
                     VNFSDSRFDDADLSGCNLSGASLKNTSFKNSVLEECDLSFSDLTNSTISASFDRANFS
                     GANLNSASFIGSSFKETPPDLKDAQLAGAIIVPGMELRGAILEQDNISLSVQNNSIGL
                     ACCQIHIPVGSENINSVLDSSRVPDTSVMRTINSVDSKYNDEKVRSVEDLIRTLPVDI
                     FDKFNPYATLSLSNAFSIPPYLASSYVQEWLQNISGRYYNTMTDWWSKYPPGDMRDGL
                     YLKI"
     gene            841..>1053
                     /locus_tag="ACHOQY_005359"
     CDS             841..>1053
                     /locus_tag="ACHOQY_005359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681225.1"
                     /translation="MNDGAFLQAGIYFEKHPEKMLSCNDVFIQMAAYGMQYEGAKNQF
                     MELYDKYLQYPNVNKIAQQSDFGRGDG"
BASE COUNT          317 a          172 c          230 g          334 t
ORIGIN      
        1 gagcaaagct gagcttactg ctgacttaat gtggttgtca gctaaccgaa cgggggaaga
       61 gtctgctgaa caattaaatt actctggatg tgacttgtca ggtctaagtc ttgcagggct
      121 gaattttgca tcagtaaatt tttctgattc cagatttgat gatgctgatc ttagtggctg
      181 taacctgtca ggtgcatccc tgaaaaacac atctttcaag aattctgttc tggaggagtg
      241 tgatttatct ttctctgatt tgacaaatag cacaatcagc gcatcgtttg atagagctaa
      301 ttttagtggt gctaatctta acagcgcatc gtttattggt tcttcattta aagaaacacc
      361 tccagacctg aaagatgcac agttggcggg ggctattata gttcccggga tggagcttag
      421 aggtgctatt ttagaacaag ataatatatc tttatctgtg caaaataata gcattggttt
      481 agcttgttgt cagatacata ttccggtggg ttcagagaac ataaactctg ttcttgacag
      541 cagcagagtt ccagatactt ctgtcatgcg aacaattaat tcagttgatt caaaatataa
      601 cgatgaaaaa gttcgatctg tggaggacct gataagaaca ttaccagtgg atatatttga
      661 taaatttaat ccctatgcga cattgtcgtt gagtaatgct ttcagtattc ctccatatct
      721 tgcaagttct tatgttcagg aatggcttca gaatatatcc ggtcgttatt ataatactat
      781 gacagactgg tggtctaagt atcctccggg cgatatgaga gatggcctgt atttaaaaat
      841 atgaacgatg gtgcattttt acaggctgga atatattttg aaaaacatcc agaaaaaatg
      901 ttgtcgtgca atgatgtatt tatacaaatg gctgcttatg gtatgcagta tgaaggagca
      961 aaaaatcagt tcatggaatt atatgataaa tatctgcaat accctaatgt aaataaaatt
     1021 gcacagcaaa gcgatttcgg tagaggtgat ggc
//
LOCUS       DAZKGS010000474         1038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKGS010000474 DAZKGS010000000
VERSION     DAZKGS010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..346
                     /locus_tag="ACHOQY_005360"
     CDS             170..346
                     /locus_tag="ACHOQY_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681226.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            343..840
                     /locus_tag="ACHOQY_005361"
                     /pseudo
     CDS             343..840
                     /locus_tag="ACHOQY_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT          328 a          175 c          281 g          254 t
ORIGIN      
        1 aagtttaaaa taattattga aacgggaata gccggtggag atttcgagga tgaattcgaa
       61 gtggatgatg atgcgacgcc tgatgaaata catgacgaag caaaagatat tttctttaac
      121 tactgcaatt attcatatca cgaaataaaa gacgaagagg aagaacaaaa tggctgattt
      181 tggttcaact aaatacagcg tcagttttga agaatggcat gaactgttaa tggaatatgc
      241 agagttacgt ggtggcagtg ctgctgatgc tgaagcatgg cgtgatgatt atgaagcagg
      301 aaaaaatccg gtcgaagcat attgtgatga gtggggcgat gaatgagcga gattgactat
      361 caggcgctgc gtgaagcagc agagaaagca actaaaggat gctacatcgt agggcataca
      421 tcgggcaatc agcatgggaa tataacagga gtttttgttt gtcaaaaatg gaaaggagag
      481 cccggtggcg taattgcaga atgtcatgtt aactgcctgg ttgaaacaga tgttcaggct
      541 tatgcaaacg ctgaatttat tgctgctttt aatccaaatg ttgcgctggc actactggat
      601 gaacgggaag cccaaaacaa acgaattgca gagctgaaag ctaatcttgt ggcgctggcg
      661 gcggagaatg cggtaataaa gtctgcaatt ccagaaccac gggatattga ggacgacaat
      721 gacaatatgg atgacgtatc tcttgctgaa gatttcgggt tcaatcatgc aatagaacgg
      781 atgaggagac ggattcctga aactccggcc accgacgctt tcctggctga agtacgggcg
      841 gggttgttta acgagctttg cgcggcgttt gtcaggtacg aaaaagttgc aggactggat
      901 gatagcgata cagtaacgct taaggaagcg acagaagcat tgctggattg tgcggaacag
      961 cttatcgcgc ctgaataatt aaatttagtt aaaaatttaa tccttaaccg gagggatttc
     1021 tgcaccctca aaacatca
//
LOCUS       DAZKGS010000475         1036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKGS010000475 DAZKGS010000000
VERSION     DAZKGS010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            445..>1036
                     /gene="pphA"
                     /locus_tag="ACHOQY_005362"
     CDS             445..>1036
                     /gene="pphA"
                     /locus_tag="ACHOQY_005362"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9681227.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIG"
BASE COUNT          291 a          185 c          252 g          308 t
ORIGIN      
        1 aagaacgtgc cacggctggc tgatggacgt tcgatagcgc gagtttgaat gaaaatcagc
       61 cggagatgat tttacataat tgctacggaa ttattcaata caggaattgc ttgtgtatgc
      121 atggattgac ctgaaatatt cccgaaaatt tctctaaaaa actcgaaaaa aatggtaact
      181 aattgaatgt attaatatgt aatagtacgt gttagagatt aaaagatgag cataaattta
      241 tttaacacat taattctaaa agattttgtt gtttgttgac gaaaacagga atcgtgttcg
      301 gtctcttttt atctgttaaa agccagaagc atttccttcg ctgactttat agtcaaccat
      361 aacacacact ctactgtctg agtccagcgt tttttaacat tcttgttaag attatgtgat
      421 ctttagcgcg ggaggaaaat attgatgaaa cagcctgcgc ccgtttatca gagaattgcg
      481 ggtcatcaat ggcgacatat ctggctttct ggcgatatac acggttgtct tgagcagttg
      541 cgccgcaaat tatggcattg tcgttttgat ccgtggcgag atttacttat ctcagtagga
      601 gacgttatcg atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg
      661 gtttgtgcgg taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag
      721 atgtctttgt ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag
      781 aaacaagcga aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac
      841 agtcgtaccg gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg
      901 caaaaggacg ttgatttgca tcaggtcttg tggagccgct cgcgattagg tgaacgccaa
      961 aaagggcagg gaattacagg tgctgatcat ttctggtttg gtcatacacc gttgcgacat
     1021 cgcgtggata ttggca
//
LOCUS       DAZKGS010000476         1031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKGS010000476 DAZKGS010000000
VERSION     DAZKGS010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..407
                     /locus_tag="ACHOQY_005363"
     CDS             39..407
                     /locus_tag="ACHOQY_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP9681228.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            480..644
                     /locus_tag="ACHOQY_005364"
     CDS             480..644
                     /locus_tag="ACHOQY_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP9681229.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            613..777
                     /gene="kil"
                     /locus_tag="ACHOQY_005365"
     CDS             613..777
                     /gene="kil"
                     /locus_tag="ACHOQY_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9681230.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            832..>1031
                     /locus_tag="ACHOQY_005366"
     CDS             832..>1031
                     /locus_tag="ACHOQY_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9681231.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAF"
BASE COUNT          316 a          224 c          227 g          264 t
ORIGIN      
        1 aatgtgaggg cgattttttt ctgtgaggat atgaacagat gtcaaacatc aaaaaataca
       61 tcattgatta cgactggaaa acatcaatag aaattgaaat cgaccatgac ttaatgacag
      121 aggaaaaact tcaccagatt aataatttct ggtcagactc tgaataccga ctcaataaac
      181 acggctctgt attaaatgct gtattaatca tgctggcgca acatgctctg cttatagcaa
      241 tttcaagcga cttaaatgca tatggtgttg tgtgtgagtt cgactggaat gatggaaatg
      301 gtcaggaagg atggcctcca atggatggta gcgaaggaat aagaattacc gatatcgata
      361 catcaggaat atttgatcca gatgatatga ctatcaaagc cgcctgagcg cggcgttacc
      421 gcataccaat tacgcttcac tcgaggcgtt tttcgttatg tataaataag gagcacacca
      481 tgcaatatgc cattgcaggg tggcctgttg ctggctgccc ttccgaatct ttacttgaac
      541 gaattacccg taaattacgt gacggatgga aacgccttat cgacatactt aatcagccag
      601 gagtcccgaa aaatggatca aacaattatg gctatccaga ctaaattcac tatcgccact
      661 tttattggcg atgaaaagat gtttcgtgag gccgtcgacg cttataaaaa atggatatta
      721 atacagaaac tgagatcaag caaaagcatt cactaccccc ctttcctgtt ttcctaatca
      781 gcctggcatt tcgcgggcga tattttcaca gccattttca ggagttcagc catgaacgct
      841 tattacattc aggatcgtct tgaggctcag agctgggcgc gtcactacca gcagatcgcc
      901 cgtgaagaga aagaggcaga actggcagac gacatggaaa aaggcctgcc ccagcacctg
      961 tttgaatcgc tatgcatcga tcatttgcaa cgccacgggg ccagcaaaaa agccattacc
     1021 cgtgcgtttg a
//
LOCUS       DAZKGS010000477         1028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKGS010000477 DAZKGS010000000
VERSION     DAZKGS010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..279
                     /gene="dicB"
                     /locus_tag="ACHOQY_005367"
     CDS             91..279
                     /gene="dicB"
                     /locus_tag="ACHOQY_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9681232.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            276..464
                     /locus_tag="ACHOQY_005368"
     CDS             276..464
                     /locus_tag="ACHOQY_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9681233.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            559..>1028
                     /locus_tag="ACHOQY_005369"
     CDS             559..>1028
                     /locus_tag="ACHOQY_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9681234.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMAIDAQELLQEDVATFDGHIVEALMKT"
BASE COUNT          294 a          232 c          237 g          265 t
ORIGIN      
        1 tctctgtatc cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcaccgc
       61 atcacaaaat tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg
      121 tctgaaggtt gttttgatat tggtgttctg ctcagtaacc gggagtttac tgaagatgcc
      181 attaatatga ggaaatatga gccttatctg ctcaatgata attccatact ttcccgaatt
      241 gctcttcttg aacttggtat tttcggagaa cgtcaataac ttcagcattt gcactgatga
      301 tgacggtttt tcttataacg ggtgaatcac agaatgtgat taccggaatt tatgccagta
      361 aagaatcctg cctccaggca agagacgagc aaaaaatttc tggtgaatgc ctcccggtaa
      421 aaaaagtatc gctgtacctg aataacgaaa caccggctgg ataaccctcc agccatatta
      481 acaccatacc aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct
      541 gtaatgaggt aaaacaaaat gagtaaagtc tttatttgcg ccgccattcc ggacgaacag
      601 gcaataaagg aagaaggtgc agtcgctgta gccactgcca ttgaagccgg tgatgaacgt
      661 cgcgcccgcg caaaatttca ctggcaattc ctggaacatt atccggctgc tcaggactgc
      721 gcttataaat ttcttgtctg cgaggataaa cccggtatac cccgccctgc cctcgattcc
      781 tgggatgctg aatatatgca ggaaaaccgc tgggatgagg cgtctgcttc ctttgtcccg
      841 gttgagactg aatcagatcc gatgaacgtc acttttgaca agctggcccc tgaagtacag
      901 aacgctgtca tggttaagtt cgacacatgt gaaaacatca ccgttgatat ggcgattgac
      961 gcgcaggaat tactgcaaga agacgtggct acctttgacg ggcacatcgt tgaagcactg
     1021 atgaaaac
//
LOCUS       DAZKGS010000478         1022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKGS010000478 DAZKGS010000000
VERSION     DAZKGS010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..998)
                     /locus_tag="ACHOQY_005370"
     CDS             complement(<1..998)
                     /locus_tag="ACHOQY_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681235.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLG"
BASE COUNT          207 a          260 c          238 g          317 t
ORIGIN      
        1 tgcccgagaa gacgttgagc aaacttatcg ccaatctgat tccggtatag cctcgccgac
       61 aggctgcgca gttcatggaa tgttggcggg tctccatcaa atgagagtcc agatgcattt
      121 ctcgcctttg taaaatactt agatactgtt ttcggggaaa gcggttcgtg atgcgttgat
      181 gcaattattg tttcactgcc gctggcctcc ctgcatttct gtagtgtatc agccaatgag
      241 atattgagcg cgtcaatcgt tagcgtcagc ggaatggcga gcttagcccc tgttttaccc
      301 tgctcaatgt gaagatggtt gtcgtttatg tctgaccatt tcattctgca taaatcgcct
      361 actctctgcc ctgtaacgac agccaaatcc attgccagcc tcagccagat tgggagcggt
      421 tcggctgcat ggtaaatcgc gacatattca ttagctgtca gtcttgaacg ccttacttct
      481 gattttgctg tgcgggttgc tgttaccgga ttcgtagcca catgcccctc ggctattgcc
      541 tcacgaaaaa cgtcaacaag ggttgacctg attaattttg cggaagccgc tttaccttct
      601 gctacgtagg tgtttagcat tgctgccacc tctttcgttg atatgtcagc gagcggtttg
      661 tccggcaatt ttcttcggat tgccctgatt ttgctggcgt agtcgagtag agttttcggc
      721 ctgatacccc tctcgctgag gattgtttca tatcggtcaa gccacgcatg aagagtgatt
      781 gcgtcagcgc ctttaattct gtctatcagt gactcacgcc tgttcccgga tagcaactca
      841 atattggcct gtattgcttc agtgattgct atcctcctgt ctcggcctaa tccgaactct
      901 ttacccgtcc ttgggtccct gtagcagtaa tatccattgt ttcttatata aaggttaggg
      961 ggtaaatccc ggcgctcatg acttcgcctt cttcccattt ctgatcctct tcaaaaggct
     1021 ac
//
LOCUS       DAZKGS010000479         1012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKGS010000479 DAZKGS010000000
VERSION     DAZKGS010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1012)
                     /locus_tag="ACHOQY_005371"
     CDS             complement(<1..>1012)
                     /locus_tag="ACHOQY_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP9681236.1"
                     /translation="QLRDINTLESFKKPQDYFFYQQEMLLRWNYAAASDQVRMNILKE
                     YGGIYTDTDILPAYSDEVSQIINKKSDGDMFFEDLKLRRFISEAILSLIKGEKYSIKH
                     DSLDEKTRNQLNAILSEIEKLTIDNYFKPVETTVIRDSFKIFKRYQKWSENNWNIRGN
                     NNFMLTHKGSKCIDFIQSGQKKQYLELQRIRDNISYNNFFYTTNDLKSLDNVEIGGIP
                     AKKYLEHGLFSEYRQDGTIPYVVSTLNISGPDMIMRQMKKYYKSLGRIGEVHIKDNKL
                     SDMNFMGVYASSDKENKSFNWLNPVSVGVNDITPDDESSWAVRNNDINKILFEKINCH
                     VPE"
BASE COUNT          300 a          182 c          122 g          408 t
ORIGIN      
        1 ttcgggtaca tgacagttta ttttttcgaa taaaatttta ttaatatcat tatttctaac
       61 agcccatgag ctttcatcat ccggtgtaat atcattaaca ccaacactca ctggattcag
      121 ccagttaaaa cttttatttt ctttatctga acttgcatat acccccataa aattcatatc
      181 acttaattta ttgtctttaa tatgtacttc acctatgcgc cctagagatt tatagtattt
      241 tttcatttgt cgcataatca tgtctgggcc agatatattt agagtactta ctacataagg
      301 aattgtaccg tcctgtcgat attcagaaaa caaaccgtgt tctaaatatt tttttgcagg
      361 tattcctcct atttccacgt tatctaaaga ttttagatca tttgtggtat aaaagaaatt
      421 attataactt atattatctc tgatgcgctg aagttctaaa tattgttttt tctgtcctga
      481 ttgaataaaa tcaatacatt tacttccctt gtgagttagc atgaaattat tattccctct
      541 aatattccag ttgttttctg accatttttg atatctttta aaaattttaa aactatctct
      601 aataactgtt gtctcaacag gtttaaaata attatctatc gtcagctttt ctatttcact
      661 caatatagca tttaactgat tacgtgtttt ttcatctaag ctatcatgct taattgagta
      721 cttttcccct tttatgaggg acagaatagc ttctgatata aaccttctta gttttaaatc
      781 ttcaaaaaac atatctccat cacttttttt atttattatc tgtgacactt catctgaata
      841 tgcaggaaga atatcagtat cggtataaat ccctccatac tctttcaata tattcattct
      901 tacctgatct gatgcagcgg cgtagttcca tcttaatagc atctcttgtt gataaaaaaa
      961 ataatcttgt ggttttttaa atgattccaa tgtgttaata tctctcagtt ga
//
LOCUS       DAZKGS010000480         1010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKGS010000480 DAZKGS010000000
VERSION     DAZKGS010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(242..613)
                     /locus_tag="ACHOQY_005372"
     CDS             complement(242..613)
                     /locus_tag="ACHOQY_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9681237.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(603..974)
                     /locus_tag="ACHOQY_005373"
     CDS             complement(603..974)
                     /locus_tag="ACHOQY_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9681238.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
BASE COUNT          302 a          281 c          169 g          258 t
ORIGIN      
        1 agccaggaca acagacaccg tcaacaatga aaaagccact accttatgcc aactgagagt
       61 tgacagtgac agtactaaca caatccacat aagcactcct tttatttaat gatgaagatt
      121 gattatcaat attttcaatt caccaggcaa cattttatct accttccaca atacatgacc
      181 atcaaaaaat aaacatgtta attataaaca cagaaaacat aaccctcatc actatatatc
      241 cctaccgcat atccatatct aaccggacat tcagagccat aagcattcct tcaataatac
      301 cttccgcttt ataaagcctt ttaccaataa gcccatcaga acatctatgc ttacgtgcaa
      361 gagccataaa tgtcattcca cctacgtaat aatccaccaa caaatcgtgc aaatactgat
      421 tattcttgtt taatcgtgcc atacaaccac aaattatcat ggcgtcatca tcagaacact
      481 gaggacgtga tttcacttta gtcgggatta atcctttaaa accagcagct atcgagggcc
      541 atgttacatc ttcatgatta tttgctgccc atgcccccca tcgctcaaga accatctgga
      601 tatcacgcgc catcgttacc acctctgatt tcgtaaatct tcacgcccag ccgtccacct
      661 ggcacaggct gaccgcgcac aatattgatt tcatcaaact gctcgtcatc aatgagcact
      721 tccgcatgcg tcagcgcatc cagcggtgct ttcagaatgt tgtccaggtc acggcggcgc
      781 ttatccggtg gttctgcaat aatttttatt gccagccgtc cggacaggct taatttcagc
      841 cgctgctggc gaacaataag cgccacagcc cggcgataac gctcaccggc ttttgataca
      901 aaatatgtgc tgccacgacg tcgccagtag gtgttcaccg tcggcgggta aggcaaaaca
      961 aactctatac gcatcagtaa cctcttttac ccgagcacgc cggttgcaaa
//
LOCUS       DAZKGS010000481         1009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKGS010000481 DAZKGS010000000
VERSION     DAZKGS010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1009
                     /locus_tag="ACHOQY_005374"
     rRNA            <1..>1009
                     /locus_tag="ACHOQY_005374"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          239 a          229 c          319 g          222 t
ORIGIN      
        1 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
       61 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
      121 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
      181 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
      241 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
      301 tttgatgttc taacgttgac ccgtaatccg ggttgcggac agtgtctggt gggtagtttg
      361 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
      421 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
      481 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
      541 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
      601 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
      661 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
      721 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
      781 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
      841 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgccccga gatgagttct
      901 ccctgactcc ttgagagtcc tgaaggaacg ttgaagacga cgacgttgat aggccgggtg
      961 tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga accgtgagg
//
LOCUS       DAZKGS010000482         1007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKGS010000482 DAZKGS010000000
VERSION     DAZKGS010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..286
                     /locus_tag="ACHOQY_005375"
     CDS             <1..286
                     /locus_tag="ACHOQY_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9681239.1"
                     /translation="EMPPSAVMSPGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDA
                     AWRLAQFTHHKKGDTGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            283..>1007
                     /locus_tag="ACHOQY_005376"
     CDS             283..>1007
                     /locus_tag="ACHOQY_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9681240.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVR"
BASE COUNT          221 a          263 c          316 g          207 t
ORIGIN      
        1 ggagatgcca ccgtccgcgg tgatgtctcc tggtattgaa tatatggagg atggtcttcc
       61 cgatccggtg aaagccatgg ggcggatcct ggtggaaaac ctctgcattg atccgaaact
      121 ggcactggat gcggcctggc gtctggcgca gttcacgcac cataaaaaag gggataccgg
      181 gaaaaaatcg gcaaaaggtg atgccgcgaa aaaagcggct aaccgttttg cggtgccacc
      241 gccaccccga ctggtggtga ataatgataa tgagggcaac ggatgatacc tgtatggagc
      301 acagcctgcc cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggatccg
      361 atttatccgg acgaggccgc acatgccctg gcgattttta aacaactgcg gattgtggat
      421 gcacctggta gccctacgtt cggggagtcc tgtgcaccgt gggtgtttga cctggtggcg
      481 gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc atatcaagga agtttttatc
      541 cttatcccca agaaaaactc gaagtccacg ctggctgcgg ggatcatgat gactgcactg
      601 ttactgaact ggcggcaggc ggcgggttac acgattctgg ccccgactgt ggaggtggcg
      661 gccaacgcct tcaaccctgc ccgggatatg gtacgacgtg acgatgatct ggatgacctc
      721 tgccaggtac agacccatat ccggaccatc acccaccggg tgacggacac caccctgaag
      781 gtggtggcag ccgatccgaa tacggtgtcc ggtatcaagt ccgtgggtac gctgattgat
      841 gagttgtggc tgtttggcaa gcagtgcaag gcggaggaca tgttacgtga agccataggc
      901 ggccttgcct cccgcccgga agggtttgtg gtgtatacga ccacccagtc gaatgaaccg
      961 cccgccgggg tgttcagaca gaaactgcag tacgcccggg atgtgcg
//
LOCUS       DAZKGS010000483          995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKGS010000483 DAZKGS010000000
VERSION     DAZKGS010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..848)
                     /locus_tag="ACHOQY_005377"
                     /pseudo
     CDS             complement(1..848)
                     /locus_tag="ACHOQY_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            825..995
                     /locus_tag="ACHOQY_005378"
                     /pseudo
     CDS             825..995
                     /locus_tag="ACHOQY_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          238 a          291 c          259 g          207 t
ORIGIN      
        1 ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg agaggtcagc
       61 acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtacgtggg tggcggcgct
      121 catgtcaaac tcaccctgca tccagcagac ggccagcaga acgtttttgg gatttttctg
      181 caatgccgct tttgtgcggg aaatcagatc ctgatataac ggcttgccca ccccccagcg
      241 tgccgaatcc tgactggccc ccgtggactc gctgaatgtc ccctccgcgc cctgggtaaa
      301 tgccgaacca ccacgacagc atggtaccag caggatcccc gcgttattcg ggatatacgg
      361 gagcagtttt ttggcaatat gtaaaccctg gccgacacag ccgtactgcc ctttgctcag
      421 gtcagccctc ggatgattca gcgtactcat atcctgcaca tcatgcagac agtggtcagc
      481 cggaataata tcgttatatc tgcaggcagc cccgcccggc gtcactgtac tgcggcgcgc
      541 cagctgttta atgcgcggat ccggagcatc gtatgaatcc ggcagcggaa gcccttcacc
      601 gtaagccctt caccgtaagc catggcattg gattgcccgg ccagtacgat gacgtagtac
      661 caatccggct cagatgaagg gccgacctgt ggctctcctt caatagccac cgcctgcatc
      721 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      781 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc gtaatgttta
      841 atcgacatcg aatttctccc gtgtagagga acagagttaa aaagccggaa gcggaatcaa
      901 atcacaggat gaccatctgc cagtggcagg tcataaaaaa aaggctgcgc aatgcgcagc
      961 cagaactcac aaggaaaatg ataaaaggaa taaca
//
LOCUS       DAZKGS010000484          995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKGS010000484 DAZKGS010000000
VERSION     DAZKGS010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>995
                     /locus_tag="ACHOQY_005379"
     CDS             <1..>995
                     /locus_tag="ACHOQY_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9681241.1"
                     /translation="PVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYFLFTDKDGE
                     KYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQPEPVKVTA
                     DEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQESE
                     QNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATY
                     QETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADHSIKVNGHQE
                     ITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGG"
BASE COUNT          335 a          239 c          255 g          166 t
ORIGIN      
        1 ccagtaccag aaactgatgc agtggaaacg gcaattccgg gcaacgaaaa aaccgaatgc
       61 aaagtggaag tcgaaccatc tgtagagcgt gaggggccgt tctacttcct cttcaccgac
      121 aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg gtctggaaaa agcactagcc
      181 ttgggagcta cggaaatcac aaaagaggaa tacttcgcac gtaaaaacgg cacgtactca
      241 ggttcacaac aaaatactgg tgcatctgac acgaccgcac aaccagagcc ggtaaaagtt
      301 accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccgat
      361 aagttgcttg ctgcctctcg cggagaattt gttgcaggga ttagcgaccc gaatgatccg
      421 aaatgggtaa aggggattga aacccgcgat tctgtaaacc agaaccagca agaatcggaa
      481 cagaacgacc agaaagcgga acaaaacagc ccaaatacgc aacaaaacga gccagaaacg
      541 aaacaacctg agccagtagc gcaacaggaa ccggaaaaag tctgcaccgc ctgcggtcag
      601 accggcggcg gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac ataccaggaa
      661 acattcgatg aagagtatca ggttgaagtt caggaagatg atccggagga aatggaaggc
      721 gctgaacatc cacacaagga gaacactgac ggcaatcagc atcacgatag cgataatgaa
      781 actggcgaga cggcagatca ctcaattaag gtgaacggtc atcaagaaat cacatccacc
      841 agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc
      901 ccgatcatct caataggtgc aatatttttc gatccgcaaa ccggagatat ggggccggaa
      961 tttagtaaga ctatcgatct ggaaactgct ggcgg
//
LOCUS       DAZKGS010000485          992 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKGS010000485 DAZKGS010000000
VERSION     DAZKGS010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            123..908
                     /locus_tag="ACHOQY_005380"
     CDS             123..908
                     /locus_tag="ACHOQY_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681242.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          323 a          191 c          210 g          268 t
ORIGIN      
        1 tattgattta atgaatttat atatactgtt atttttgttt gttctgattc aagtggagta
       61 tataattaaa aatcgttagc taaacattac catgattatg acaggcgagg gagtgtccaa
      121 ttatgaattc aaaaaagctt tgttgcatat gtgtgttatt ctcgctgctt gcaggatgtg
      181 cctctgaatc ttctattgat gaaaagaaga aaaaagctca agtcacacaa agtaatatta
      241 ataaaaacac tccccagcaa ctgacagaca aagatttatt cggtaatgaa accactctgg
      301 ccgtatccga agaggatatt caagctgcgc ttgatggaga tgagttccgc gttcccctta
      361 attctcctgt aatacttgtt caatccggca accgcgcacc ggaaaccatt atgcaggagg
      421 agatgcgaaa atattatact gtttccacat tctctggtat cccggacagg caaaagcctc
      481 tgacttgtaa caaaaacaaa gataaaaatg agaacgagga tgttgccagt gctgagaata
      541 tgaactggat gcaggcactg cgttttgtgg ctgcaaaagg gcatcagaaa gcgattattg
      601 ttttccagga tacgttgcag acaggaaaat atgactctgc gctgaaatca acagtatggt
      661 ccgactataa aaatgaaaaa ctcaccgacg ctatctccct gcgctacctg gtacgtttca
      721 cgctggtgga tgtggcaaca ggtgagtggg ctacctggtc gccggtgaat tatgaatata
      781 aagtgcttcc accattgccc gacaagaatg aagccagtac gactgatatg acagagcagc
      841 aaatcatgca acttaagcaa aagacctata aagcgatggt aaaagatttg gttaaccgct
      901 atcaataata aattatatct gccgccagga atttctggcg gcaataatac aaaattcttt
      961 gcataagaaa tttttcttga caaagacagt gc
//
LOCUS       DAZKGS010000486          990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKGS010000486 DAZKGS010000000
VERSION     DAZKGS010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..582
                     /locus_tag="ACHOQY_005381"
                     /pseudo
     CDS             1..582
                     /locus_tag="ACHOQY_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            556..>990
                     /locus_tag="ACHOQY_005382"
     CDS             556..>990
                     /locus_tag="ACHOQY_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9681243.1"
                     /translation="MNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKRLNL
                     GGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSHSEM
                     IEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTIKALQ"
BASE COUNT          282 a          195 c          303 g          210 t
ORIGIN      
        1 cgccgctcat ccgggtcgag acagagaacg gcgagcggga gaaagcgcag cgccgcagcc
       61 tggcggagaa gttacagcag gaagggagcg aagacggtca cggtgtggtg ttcccggctg
      121 aactggtgcg gctgctggac agactggagg aagaaatccg ggcagaccgc gtgagtgacg
      181 aaagccgggc gtggcttgcg cagtgcgggc tgacggtgga gcggctggcg gcacagatag
      241 agccggttta tttgcctgag cgaaaaatac atctttatca ctgtgaccac cggggcctgc
      301 cgctggcgct tatcagtgaa gacggcaata cggcgtggag cgcggaatat gatgaatggg
      361 gcaaccagct taatgaggag aacccgcatc acctgcacca gccgtaccgg ctgccggggc
      421 agcagtatga taaggagtcg gggctgtact acaaccggaa ccggtactac gatccgttgc
      481 aggggcggta tatcactcag gacccgatag ggctggaggg ggggatggag tctgtatgcg
      541 tacccgctga atccggtgaa tggtattgat ccattagggt taagtcccgc agatgtagcg
      601 ctaataagaa gaaaagatca actaaaccat caaagagcat gggatatatt atctgatact
      661 tatgaagata tgaagagatt aaatttaggt gggactgatc aatttttcca ttgtatggca
      721 ttttgtcgag tgtctaaatt aaatgacgct ggtgttagcc gatcggcgaa agggctgggt
      781 tatgaaaaag agattagaga ttacgggtta aatctgttcg gtatgtacgg cagaaaagta
      841 aagctatccc attctgaaat gattgaagat aataaaaaag acttggctgt aaatgaccat
      901 gggttgacat gtccatcaac aacagattgc tcagatagat gtagtgatta tattaatcca
      961 gagcataaaa aaacgataaa ggctttacaa
//
LOCUS       DAZKGS010000487          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKGS010000487 DAZKGS010000000
VERSION     DAZKGS010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..267
                     /locus_tag="ACHOQY_005383"
     CDS             <1..267
                     /locus_tag="ACHOQY_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9681244.1"
                     /translation="EKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRR
                     GACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            427..564
                     /locus_tag="ACHOQY_005384"
     CDS             427..564
                     /locus_tag="ACHOQY_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681245.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            566..928
                     /locus_tag="ACHOQY_005385"
                     /pseudo
     CDS             566..928
                     /locus_tag="ACHOQY_005385"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            900..971
                     /locus_tag="ACHOQY_005386"
                     /pseudo
     CDS             900..971
                     /locus_tag="ACHOQY_005386"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
BASE COUNT          254 a          228 c          282 g          224 t
ORIGIN      
        1 gagaaaaaca tcagagtgcc actgaccgaa ccccagaaag cggggattgc gtcattctgt
       61 ccgtacaaca ttggccccgg taagtgtttc ccgtcgacgt tttacagacg gattaatgcc
      121 ggtgaccgca ggggagcatg cgaggcgatt cgctggtgga ttaaggacgg tggcagagac
      181 tgccgtattc gctcaaacaa ctgctacggt caggtctcac ggcgtgacca ggagagcgcg
      241 ctggcgtgct ggggaattga cagataagca gaatattttg ctgaaaaatg cgatttgctc
      301 acgcggacgg ataacacgaa atcctgcgaa ctgacaaaaa ctaagtgaat aaaagtaaaa
      361 accccgtttg ttggctgcaa gcggggtttt gtgtttcctg actccggaaa agtcaaagga
      421 gaaagtgtgt ttgattttag caaactgatt cgggagattc gaatgatggc tgaaaaatta
      481 tccacctgga agttcatcct tatctggctg gtgtttgtga ttatggcttc cggttatttc
      541 attggtcaga tacgctggtg gtgaaatgaa ccgcgtactg tgcgtggtca tcattgccct
      601 gctggtggcc tgtggtgcgc ttagtctggg gctgaatcat taccgtgata acgccataac
      661 ctacaaagag cagcgcgata aaaaagtcag tgagctggag ctggcaaatg caaccattac
      721 tgatatgcag cagcgccagc gtgatgttgc tgcacttgat gccagatact cgagggaatt
      781 agccgatgcg agagctgaaa atgaaactct gcgcgctgat gttgccgctg gtcgtaagcg
      841 cctgcggatc aacgccacct gccccggtac cgtgcgtgaa gccaccggca cctccggcgt
      901 ggataatgca accggccccc gactggcaga caccgctgaa cgggattatt tcatcctcag
      961 agaacgattg atgacaatgc agaagcag
//
LOCUS       DAZKGS010000488          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKGS010000488 DAZKGS010000000
VERSION     DAZKGS010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            254..856
                     /gene="fimB"
                     /locus_tag="ACHOQY_005387"
     CDS             254..856
                     /gene="fimB"
                     /locus_tag="ACHOQY_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9681246.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          301 a          174 c          209 g          304 t
ORIGIN      
        1 gagggttatt attagattcg tatccgattg ataaatatat aaaggtacat agcatgcaag
       61 agcatagcgt ttgtatggca acgttattat aagtaactgt tgctactcca tttaagttca
      121 ctcagaagaa ctggtccact tacgttagtt attaagcaaa cgttcgcttt tataaacata
      181 atcaggataa aaatgttgga ttattgctaa cccagcacag ctagtgcgcg tctgtaatta
      241 taagggaaaa acgatgaaga ataaggctga taacaaaaaa aggaacttcc tgacccatag
      301 tgaaatcgaa tcactcctta aagcagcaaa taccgggcct catgcagcac gtaattattg
      361 tctgactttg ctttgtttta ttcatggttt ccgggcgagt gaaatttgtc gattgaggat
      421 ttcggatatt gatcttaagg caaagtgtat atatatccat cgattaaaaa aaggcttttc
      481 aacaacgcac ccgctattga acaaagaagt tcaggcttta aaaaactggt tgagtatccg
      541 tacttcgtac ccgcatgctg agagcgaatg ggtattttta tcgcgtaagg gaaatccgct
      601 ttctcggcaa cagttttacc atattatctc gacttccggt ggtaatgccg ggttgtcact
      661 ggagattcat ccgcacatgt tacgccattc gtgtggtttt gctttggcga atatgggaat
      721 agatacgcga cttatccagg attatcttgg gcatcgcaat attcgtcata ctgtctggta
      781 taccgccagc aatgcagggc gtttttacgg catctgggat agagccagag gacgacagcg
      841 tcacgctgtt ttatagagat agttgagata ccagggatgg tgttaaaaac tacattattc
      901 ttacgatata tcaaaaatga ttaaccattt attaattgat gttattgtaa gtgtgattat
      961 taattttcat aataaacatt aagttaac
//
LOCUS       DAZKGS010000489          976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKGS010000489 DAZKGS010000000
VERSION     DAZKGS010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..835
                     /gene="yeeO"
                     /locus_tag="ACHOQY_005388"
     CDS             <1..835
                     /gene="yeeO"
                     /locus_tag="ACHOQY_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9681247.1"
                     /translation="PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFK
                     PLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINL
                     PGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFY
                     TQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVV
                     GYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVS
                     E"
BASE COUNT          198 a          197 c          281 g          300 t
ORIGIN      
        1 gccgggactg ggatttgtcg gggctgggtt gggtttaacc atttctcgtt atattggcgc
       61 agttgcaatt ttgtgggtgc tggcgattgg ttttaatcct gcgctaagga tttcgttaaa
      121 gagctatttt aaaccgctga attttagcat tatctgggaa gtcatgggga ttggtattcc
      181 agcgagtgtc gaatcagtgt tatttaccag tggtcggtta ttaacccaaa tgttcgttgc
      241 cgggatgggg accagtgtta ttgccggaaa ttttatcgcg ttttcaattg cggctcttat
      301 caacttaccc ggaagtgcgc tcggctctgc ttctacgatc attacaggcc gaaggttagg
      361 ggtagggcag atagcgcaag cagagattca gttgcggcat gtcttctggc tgtccactct
      421 tggattaacg gccatcgcct ggctaacggc tccctttgcc ggggttatgg catcgtttta
      481 cacccaggat ccacaggtta aacatgtcgt tgtgattctg atttggctaa atgctttatt
      541 tatgcctatt tggtccgcct catgggtgct acccgctgga tttaaggggg ctcgtgacgc
      601 ccgttacgct atgtgggttt ctatgttgag catgtggggt tgccgggttg tcgtcggtta
      661 tgtgctgggc atcatgcttg gctggggtgt ggttggtgtc tggatgggaa tgtttgccga
      721 ctgggctgtg cgggccgtgc tgttttactg gcgaatggtt actggacgtt ggctatggaa
      781 ataccctcga cccgaaccgc aaaagtgtga aaaaaagcca gttgtgtcgg aataaacaac
      841 aaaatgcaga ttatttcagc aaacgatttc aaatttaaaa aacaggcttt gacattgtgg
      901 gtgggcatcg ctaatattcg cctcgttctc acgattcctc tgtagttcag tcggtagaac
      961 ggcggactgt taatcc
//
LOCUS       DAZKGS010000490          975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKGS010000490 DAZKGS010000000
VERSION     DAZKGS010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..>975
                     /gene="phoE"
                     /locus_tag="ACHOQY_005389"
     CDS             67..>975
                     /gene="phoE"
                     /locus_tag="ACHOQY_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9681248.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLV"
BASE COUNT          281 a          203 c          251 g          240 t
ORIGIN      
        1 actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt aaaatcagga
       61 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgcatct
      121 gtacaggctg cagaaatata taataaagac ggtaataaac tggatgtcta tggcaaagtt
      181 aaagccatgc attatatgag tgataacgcc agtaaagatg gcgaccagag ttatatccgt
      241 tttggtttta aaggtgaaac acaaattaac gatcaactga ctggttatgg tcggtgggaa
      301 gcggagtttg ccggaaataa agcggagagt gataccgcac agcaaaaaac gcgtctcgct
      361 tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
      421 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ttcctcggcg
      481 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
      541 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
      601 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggc
      661 ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa cgagcagaac
      721 ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg ctacaggtct gaaatacgat
      781 gccaataatt tttatctggc aactttttat tctgaaacac gcaaaatgac gccaataact
      841 ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca gtttgacttt
      901 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga aggtatcggt
      961 gatgaagatc tggtc
//
LOCUS       DAZKGS010000491          973 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKGS010000491 DAZKGS010000000
VERSION     DAZKGS010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..108)
                     /gene="ybfB"
                     /locus_tag="ACHOQY_005390"
     CDS             complement(<1..108)
                     /gene="ybfB"
                     /locus_tag="ACHOQY_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFP9681249.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDV"
     gene            complement(108..>973)
                     /locus_tag="ACHOQY_005391"
     CDS             complement(108..>973)
                     /locus_tag="ACHOQY_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9681250.1"
                     /translation="VSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDI
                     WGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETD
                     NSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIG
                     RFIRFVFTGVM"
BASE COUNT          246 a          259 c          197 g          271 t
ORIGIN      
        1 cacatcacga gtggagtcta atagtgaaag tctatgcatg gaaaagaata gtattaaaag
       61 cgataaagcc agccatatag ctctgaataa aaaaataata tatttcatta catcactccg
      121 gtgaatacaa atctaataaa acgtccaatg ttatttccgg agcacttcat cttacatgtt
      181 tctaaagaat cattagacca gtcttggcag tttttaaata gccctaaatt tataggtgtg
      241 aatatatcag gcaatacaga ataacgcggc cttacctctt taaacttccc ttttaaacag
      301 ttctccacac aactattatc ggtttcaacg aggtcacaac ggccagcagc ttcttttgta
      361 tcattacttg ctttacctgg cccccataga ccatctcctt ttgattctcc ccgttggtct
      421 tggccaccac aagtatagtc ccctttacca tctgggacac aaagatattg atgatagaac
      481 ggattccccc atatatctgg accgcttctt tcaccagttc cgcccattga atgcagtggc
      541 ttaatgcatt ttagtgtttc taatcctaat ggatctatat ttgagatcgg attcaacgga
      601 tactgataaa aattccatcc ccccttcagc ccaatcggat cctgagtgat ataccgcccc
      661 tgcagcgggt cataatagcg gtggcggttg taatacaggc cggactcctc atcatactgc
      721 tgccccggca gccggataag ctgctgcagc tgatgcgggt tctcttcatt cagcaggttg
      781 ccccattcat catattctgc gcaccacgct gttgcccctt ccgtgctgat gagcgccagc
      841 ggcaggccgc gatggtcgca gtggtacagg tggatttttc gcgccggcgt gtacaccggg
      901 tccatctggt tttgcatctg ctccacggtc aggccgcacg atgccagcca gcggcggctt
      961 tcctcactca ccc
//
LOCUS       DAZKGS010000492          971 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKGS010000492 DAZKGS010000000
VERSION     DAZKGS010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..473
                     /locus_tag="ACHOQY_005392"
     CDS             111..473
                     /locus_tag="ACHOQY_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681251.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYVSGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
     gene            492..>971
                     /locus_tag="ACHOQY_005393"
     CDS             492..>971
                     /locus_tag="ACHOQY_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9681252.1"
                     /translation="MILGNDYVDLIPLFQTHSTRNFLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYIAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     "
BASE COUNT          287 a          187 c          197 g          300 t
ORIGIN      
        1 tgttctgtag aagaagtaga aagatgtgtt gaaaaatatt tttctccttc ttctccgtct
       61 acaccttccc ttaatgaatt aatcaaatta aaagctaagg agattactaa atgattattg
      121 atttagacac tctgtttcca gttcgaaaga ctttcacaga tattgtaagt tattgtactg
      181 atccgtttgc ctctgtagaa agcaaggttt ttgcttctct tcctgctgat gttgaatgcg
      241 gcgacttgat caccagcact ggcgctaaat atgtatcagg agatgatatc tatgttgtta
      301 tgagtgaatt tgtaacggct ggagaaaaca aacctgtaga tgttttacgt agcaatgctg
      361 ggcttgtatg catcaaagct gatgcgctga atgctgtaag tgaagcagca aaaacagcat
      421 taattaaaaa aggcttccag cttgaagggt tccattctgt tttcacttct taataaaagg
      481 aataataata aatgattcta ggcaatgatt acgttgattt gatccctctg ttccagacac
      541 acagcacgcg taacttcctg ctctctactc ttgattttac ggattctgta ggggttacat
      601 ctcataaagt agctgtttcc cagcttgtcg aaagcaacga atctctcttt aacaaagaga
      661 caagccgttt tagttctgaa cacaatgtta ctaagcgaga acaaggtaaa gaatggctga
      721 tcgagatccc ttacttcctg cgtgaagatg tgatccgtcc ttctgatgtt caaggcaaac
      781 gtaaacctgg cactgatttc caggaaacac tcactgatat ttatgcggaa tatatcgcta
      841 aacaccacgt agcgtatcag cgtacaaaag agagcgtatt ggctgcttct ttgttctcgg
      901 gcaaaactta tacaccgaaa actgatgatg ttcttatcga atggggcaag ctgtttaacg
      961 tatcagctat g
//
LOCUS       DAZKGS010000493          959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKGS010000493 DAZKGS010000000
VERSION     DAZKGS010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHOQY_005394"
     CDS             <1..882
                     /locus_tag="ACHOQY_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681253.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          244 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaact
//
LOCUS       DAZKGS010000494          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKGS010000494 DAZKGS010000000
VERSION     DAZKGS010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..599)
                     /locus_tag="ACHOQY_005395"
                     /pseudo
     CDS             complement(60..599)
                     /locus_tag="ACHOQY_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
BASE COUNT          279 a          230 c          193 g          255 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 actgaacgcc atttaccaca aagctgattc ttaattgccg cagtcgtggc atcattaaaa
      121 atgaatttat acctcacagg ttcttccgca ctgtttttct gaagtacttc cagttgtctg
      181 agggtttctg actcatccgc gcctttgcgg agttctttat tcagttttga caacaatgct
      241 tttttctgag cctttaaaag ctcttccttg tttttccaga cagccagttc acttttgtat
      301 ctggaatata actgcatctc ctgctggtac aggggcttca ttaataattt gtccactgcg
      361 gtttttcgtt ctccggactc ggcaagcgtc aggaaaaaaa gagagaccgg cccccgcagt
      421 gtattcagtc tgcagacatc aatcagattc tggcagacaa gtgaaacagc gcccagggca
      481 gacgctgcca caagcccaag gggagcctgc gtaatttgct gagcttcaat aacggcatca
      541 cgaatgagtg gaggcagcga actcagggga aactcatcac aaggctgacc gaatgacata
      601 gtaacctcac tatacacaga tataaaagca ctgcaattct ggctattaat agcaaaaata
      661 gcagtcaaat caatatgagg taaaattttt tgttgattta atggttaaat aataaccacg
      721 gtgtattttg ctgctgaaga aagacaaaat gaatctgtgt gtcccctcct tcccttcgga
      781 gcacatccac tattcaaatt ttacacaacc acgtacgaac aaaaagcatc atcccgggta
      841 ttgcagatac cggtttcctg ccgggaagaa tcataaacag aacaccggga ataacgcctt
      901 caggaatcac cccccctcct gaagaagaat aaaataaaga agactacagt tcctgca
//
LOCUS       DAZKGS010000495          952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKGS010000495 DAZKGS010000000
VERSION     DAZKGS010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..509
                     /locus_tag="ACHOQY_005396"
     CDS             <1..509
                     /locus_tag="ACHOQY_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9681254.1"
                     /translation="EFIGQDGAKTCEELAGKFGVSTRKVASTLAVVTATGRLARVNQN
                     GKFRYCMPGDNLPAEPKAALVTESDGKAFPQPAGAALPVREAATQEEIKTESVAVTVQ
                     SQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALRELNKHRDIVRQITDS
                     SRRVVSEK"
     gene            525..947
                     /locus_tag="ACHOQY_005397"
     CDS             525..947
                     /locus_tag="ACHOQY_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9681255.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          247 a          210 c          294 g          201 t
ORIGIN      
        1 ttgagtttat cggtcaggat ggggctaaaa cgtgtgagga actggcgggt aagttcggtg
       61 tcagtactcg caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac
      121 gcgttaatca gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcagagc
      181 cgaaagccgc gctggtaacg gaaagtgatg gtaaggcctt tcctcagcca gcaggtgctg
      241 cgttaccagt ccgggaagcc gcaacacagg aagaaataaa aacggaaagt gtggcggtca
      301 cagtgcagtc acagccgtcg ttcaccagaa agcatccgga tggtctgatt ttaccatcgc
      361 tgcatgtggc taaccgcgag ctgcgccggg caaaaggtca ggttcagaag tgggagcgag
      421 tctgcgccgc gctgcgggag ctgaacaagc accgggatat tgttcgacag attactgatt
      481 cttcccgccg tgttgtatcg gaaaagtgat tgccggaggc gcttatggca aaagtattta
      541 cacaggaaga gcgggaaaaa attaaagggc aggttgttga gctagtacgc cggagtgggc
      601 gcgagacgtt acggcaactg gaagccaaga caggtgcgac aagatatctg atgagcgttc
      661 ttgccagaga gctggttgcc agtggcgatg tatacaactc tggttacggg ttattcccat
      721 ctgaacaggc tcgtaaggac tggcaaaacg cccgcaaaaa actatcgagg gcaaaactga
      781 agaaaccggt tgtggttgat cctgacctta tctggtcatt accagacgga gaaatacgcc
      841 gctacgacag gcgtctgaac ataatctgta gcgagtgccg gaagagcgaa gctatgcagc
      901 gtgtactggc tttctatcag ggtaattttc agaaggtgct gttgtgagcc aa
//
LOCUS       DAZKGS010000496          950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKGS010000496 DAZKGS010000000
VERSION     DAZKGS010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..659)
                     /locus_tag="ACHOQY_005398"
     CDS             complement(<1..659)
                     /locus_tag="ACHOQY_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFP9681256.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARTLFGQ
                     K"
     gene            complement(800..>950)
                     /locus_tag="ACHOQY_005399"
     CDS             complement(800..>950)
                     /locus_tag="ACHOQY_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9681257.1"
                     /translation="DENGIIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWEN
                     IGGMK"
BASE COUNT          250 a          203 c          174 g          323 t
ORIGIN      
        1 ctcttttgac caaataatgt cctggccaac cagccgttat tcatatttgc ggatttttct
       61 atttcatcag gagagaagtc tttgtttttt attatgttgg ctgagttaca ggatgttaat
      121 ctgatatcat ctatttcatg aagattatgt tgaacaatgc ggtcgacaat atctgacggt
      181 gataaaaatt catctccgca cttaagaaga ggtagaccag cagaaccatg cccagacaga
      241 taaattttgt tgtattttcc tggtgttaaa tcatctggca ggatggtttt catctctgtc
      301 gctgtaatac ttatagctgc agcagcaaca acatcgctat tacttgattg tagatggctt
      361 ttatttgctc caggatatgt aaactccatt ttccttttat caaaatcttg ttttgtagca
      421 tcattcagga ataataaatc atacggtttt ttccccgttg tgactcttga aacattttgg
      481 ccaagaatat taagagtata gtcattaacg gatttcattc ctaaaaaaac aagtaatcct
      541 tctttctctt gcgctttttc gattgttctc cctgctaaag ttattgggat ggattgagat
      601 tgttttgtcg gaactgatac gctggcgcta ataggtagta gggttcttat gctaaacata
      661 caacctcttt cttaattttc gaacctgttt aggattctgt gtaaattcaa aataaacctg
      721 tctgaacatt cctaacaaac atccaccgga catgacaaca aaaaccggag ccggactccg
      781 gtttttgtga agctgtcggc tatttcattc cgccaatatt ttcccacgtc ccgtcagcac
      841 gcagaatttg cagcggtctt accacgcact gtatctgctt tttatccgca tccagtatca
      901 ccacctgcgt gattaccctg gcctgctccg ggataatgcc attctcatcg
//
LOCUS       DAZKGS010000497          946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKGS010000497 DAZKGS010000000
VERSION     DAZKGS010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(70..146)
                     /locus_tag="ACHOQY_005400"
     tRNA            complement(70..146)
                     /locus_tag="ACHOQY_005400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Arg,seq:tcg)
     gene            complement(156..231)
                     /locus_tag="ACHOQY_005401"
     tRNA            complement(156..231)
                     /locus_tag="ACHOQY_005401"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ile,seq:cat)
     gene            complement(260..>946)
                     /locus_tag="ACHOQY_005402"
     CDS             complement(260..>946)
                     /locus_tag="ACHOQY_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9681258.1"
                     /translation="VCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMHYGKVKIVDI
                     SESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGEATDMFLMMA
                     CLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHMSESLLKTKL
                     KNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVFHRYYGFPPD
                     RYVSRQGLDY"
BASE COUNT          272 a          235 c          204 g          235 t
ORIGIN      
        1 aaccactgac cgattgctta gaaggcaatt gctctgtccg actgagctaa caacgcatga
       61 tgctgataat ggaccgccat cggggacttg aaccccgcgc agccagcttc gaaggctggc
      121 gctctgtccc gatgagctaa tggcggtatg tgatatggtg gcccttgctg gatttgaacc
      181 agcgacctgg cgattatgag tcgctcgctc tcaccactga gctaaagggc cgagccaaaa
      241 aataataatc agatgaaaat caataatcaa gcccttgcct ggatacatat ctgtctggcg
      301 ggaagccata atagcggtga aatacagaaa taaagtagga cctgcttgaa taaccgcatt
      361 tttctgctat agcctgtcca tatccatgcc gggaacataa catatttaca gcaacacgca
      421 tccgctcttc cagcaacaag cgactgaaca tgcccccttc atttttcagt tttgtcttta
      481 acaaactctc actcatatgc aactgtagag caatcgcacc aagcgtccag cttgctgata
      541 tatctgtctg aattatcgcc ctgactttgg cacttatgct ggataaacat ccacttaaaa
      601 acaatgacat ccgttcatct gtttcaaaca gagacaggca ggccatcata agaaacatat
      661 ccgtggcctc tccggaaaat ccctggctgg taattaaagc cgcagccaac gcaggattgt
      721 tgggttccag caacaggtaa agcggaatgt cagtcagacg agtcctcgtc agcttatgct
      781 gactttccag atattgactt acgacggatt cgcttatatc gacaatttta actttgccat
      841 aatgcataag gaaaagctcc ctgatgcatt tggtggccag aacgactgag cctggcttaa
      901 gtgacaacgt atccttttca agaaaaatat taattgggga gcaaac
//
LOCUS       DAZKGS010000498          934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKGS010000498 DAZKGS010000000
VERSION     DAZKGS010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..223
                     /locus_tag="ACHOQY_005403"
     CDS             <1..223
                     /locus_tag="ACHOQY_005403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681259.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(336..532)
                     /locus_tag="ACHOQY_005404"
                     /pseudo
     CDS             complement(336..532)
                     /locus_tag="ACHOQY_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            708..>934
                     /locus_tag="ACHOQY_005405"
     CDS             708..>934
                     /locus_tag="ACHOQY_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9681260.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          246 a          249 c          234 g          205 t
ORIGIN      
        1 gcagcgctac gatgactccg gtgttatctg caccggagga ccgggaacat taagcggtat
       61 agcgacaaca aagaattcga aatcaaaagg gaatggtgcc ggaggcgtgg gcggtaatag
      121 gctgattttc aggtattacc ggcctgtatc tgctgaaggg ggctcgtatt tgtcggggct
      181 gcgtattccg ctcgattcga tcacggattg gcggaatacc taactgtgat tgattcaggt
      241 accattttgc agggatccgg tgcgttactg tctgacacaa caaacgcagc gggtcgctct
      301 gctccgccga ttcctgataa tccctcccca gtaacctact gcaacagtac aaatacactg
      361 acgcctgaaa cggtaaccag aatcagcacc agcagtaact cagtaaacag agcagacgat
      421 cggtcttccg gtaataaccg gaaggaacga cagcgccaca atacaggcaa cacactgtat
      481 tatgagtaat cgaattaccg caatccccgc atttcttttt gcgtatcacg atagctccct
      541 cgccacgcct tatccgtaac cggtttttta cattaaaaaa aacaacgccg ggaattcaat
      601 cgtcagtccg gagacgaccg ttcgggttat cacagagagc ctgagcaggg gggctgcgca
      661 ttttttacga tgtggctatt ccgtatgaac catacggaga tataaccatg acctacaaat
      721 acaacccctt ctggcagcaa cgtattcgtg agacggtgcg gcacgcactg aatgttcatc
      781 cccgcctgac ggcattgcgg gttgacctgc gtttcccgga tgtaccggca gcaacggacg
      841 cagctgtgat atcccgcttc atcaatgccc tgaaagcccg aatcgacgct taccagaaac
      901 gtaagcatcg ggaaggtaaa cgcgtgcatc ccac
//
LOCUS       DAZKGS010000499          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKGS010000499 DAZKGS010000000
VERSION     DAZKGS010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /locus_tag="ACHOQY_005406"
     CDS             <1..117
                     /locus_tag="ACHOQY_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HFP9681261.1"
                     /translation="RRIEYTAASLDDLNRAINDAESVLGTTSRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHOQY_005407"
     CDS             114..>931
                     /locus_tag="ACHOQY_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681262.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          191 a          216 c          322 g          202 t
ORIGIN      
        1 cgaagaattg aatatacggc ggcctctctg gatgatctta accgggcgat caatgatgcg
       61 gagtcagtgc tggggacaac aagccgccgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggtg ttccgcttcg ggagagcctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gttgcccgcg ttgcgtctgg gcaatgcccg tgcagatgat ctggtgcgca
      301 ataacgggat agcggccaat gcggtggcac tgcataagga tcatattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagact gcagcaaaaa
      421 gttttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga agggaaacgc acgtttacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggagagcacg caactgtttc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctctggta gtggacgatg ggaacggatc ccgcgtgaac
      781 tacccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggctaa tcagttttac agcgtcatgg aacggctgaa gatgctggat tccctgcagg
      901 caacacagct tcagtcggcc attgtgaaag c
//
LOCUS       DAZKGS010000500          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKGS010000500 DAZKGS010000000
VERSION     DAZKGS010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /gene="gpW"
                     /locus_tag="ACHOQY_005408"
     CDS             <1..117
                     /gene="gpW"
                     /locus_tag="ACHOQY_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9681263.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHOQY_005409"
     CDS             114..>931
                     /locus_tag="ACHOQY_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681264.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          189 a          224 c          321 g          197 t
ORIGIN      
        1 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
       61 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg ctgagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacggaat agcggctaat gcggtggctc tgcataagga tcacattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
      421 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg c
//
LOCUS       DAZKGS010000501          921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKGS010000501 DAZKGS010000000
VERSION     DAZKGS010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..452
                     /locus_tag="ACHOQY_005410"
     CDS             <1..452
                     /locus_tag="ACHOQY_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFP9681265.1"
                     /translation="VPHSNTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKEREC
                     WLVINNPLATVKLCDNNLFIADVQAADLLCTISNEAVMDRIYCVIGTIHVDIHMLRNA
                     KKDDIIDSTGYHLFGGCRLIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            483..>921
                     /locus_tag="ACHOQY_005411"
     CDS             483..>921
                     /locus_tag="ACHOQY_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFP9681266.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLL"
BASE COUNT          277 a          151 c          192 g          301 t
ORIGIN      
        1 ttgttccaca ttctaatacc attttctttg atggatatat tgctcctggg tggactgatt
       61 acccggttac agagctgact gttggtgata gtttaaggct ctatcatgtt gatgacagca
      121 aagaaagaga gtgttggctt gttattaata accctttagc aaccgtaaaa ctttgtgata
      181 ataatttatt tattgcggat gtccaggctg cagatttgtt atgtactatc agcaatgagg
      241 ctgtaatgga tcgcatttat tgtgttattg gaacaattca tgttgatata catatgttgc
      301 gcaatgccaa aaaagatgat attatcgatt caactggata tcatcttttt ggtgggtgcc
      361 ggttaatacg caataatacg actatagcat acggaagcat tgtaaaaatc aacgaagatt
      421 tttattttac tgttagttta gtatgtgatt aatcaggaat aagttatata gggagatgta
      481 ttatgtcgtc atcgttatca ggaataatac tcaatacttc gctgacaagt caccatatgt
      541 catggggcaa gtttagcaga actcttcatg tattgaatga agatatcgta gatgaaattc
      601 agcttaaagc ccgaacacaa caacgccacc atcgagtata ttcagaaatg gcggattact
      661 ccgttgtctc ctttcggggc aaagaatatt ctgttcgttt tattaaagat tacaaaacag
      721 ataattatgt gtacaaagta caaaagatga ctcaggaaag tgggtgtctc tcaagattat
      781 ttagctttct ttctggcggt gtgactaagg ctttggaaag taaactaaat gagaggcata
      841 tcactccact ttctccaacc tggtttcctc gaactccact ggagggggtc ttaacggaaa
      901 gaggattatc ttctttgtta c
//
LOCUS       DAZKGS010000502          918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKGS010000502 DAZKGS010000000
VERSION     DAZKGS010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            90..>918
                     /gene="escD"
                     /locus_tag="ACHOQY_005412"
     CDS             90..>918
                     /gene="escD"
                     /locus_tag="ACHOQY_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP9681267.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     "
BASE COUNT          277 a          189 c          175 g          277 t
ORIGIN      
        1 acagcttcct ttctactcta ttcttagttg atgtaagttc accatatttt ttctcattgt
       61 tcaaccacca ggatgaataa aatttaaaaa tgttatcctc atataaaata aaacttctca
      121 atggtgcgat gcgtaatcgg gaattacaac ttccgatggg aaacctaacg ataggcacag
      181 aagataacga tattgtttat tttccccttg aacaaggcct caatcagttt ttacttgata
      241 tacgtgagga aggtgttttc cttttatctc cggttgaatt ttggattgat ggacagccca
      301 ccccctatga agcagatcag tcattacctg tcggaaaggt catcgatata gcgggatgtt
      361 gcttcatcat tggtgatatt gaccacacct tgccattgtc tgatgtgcca gaaaggttct
      421 ctgccaaaaa tcgacgtaaa aagcgtctta tcctggctag cgtgattggt gctgcgattg
      481 cgttgtctgg cgccatcggc agttacgttt tgctttcccc aaaagctgaa cccccagcgt
      541 ttaccagagc tgatgtatac cagcagctaa aagaaaacaa acttcatgca atcactctgg
      601 tatggcatgg gaaaaatgtc gcgctttatg ggcgttgcga atcaaccact gatttaactc
      661 cattttttaa ttacctgaaa gaaaaaaata ttttttatta caacaatatc atttgcaata
      721 accaaatcat ctccgcgatt aatgatattt taacggaata cggttataag gatatcatca
      781 ttacaaaagg taataagcct ggattcttcc tgttatcggg atacattccc ccttcaccaa
      841 aatggagcga agttgaaaat ctgttactga atacgccagg ggttgctgga tgggaaatac
      901 ataacaacag taacaata
//
LOCUS       DAZKGS010000503          918 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKGS010000503 DAZKGS010000000
VERSION     DAZKGS010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(117..637)
                     /locus_tag="ACHOQY_005413"
                     /pseudo
     CDS             complement(117..637)
                     /locus_tag="ACHOQY_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..761
                     /locus_tag="ACHOQY_005414"
                     /pseudo
     CDS             681..761
                     /locus_tag="ACHOQY_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          264 a          172 c          198 g          284 t
ORIGIN      
        1 gcttttgtta aaactgcaat tatttgtaaa cataataata agtattatga cgttaaaatt
       61 taaccatacg gtaaaatgat tcaggaaaac cggttcagca tagcctaggc tgaacttttg
      121 aggatatcaa cgaacggaat ttttgaacat ttatgagtag atcgttgttg aagggcttca
      181 atgagcatgt caagttcatc aactgctggt ctacgattac gacggtttga tttaccaatc
      241 aaagcaagtt taagtagata tggacgagca cttttcgccg ggtttgatgt gtaattaatt
      301 ccgtatatag gtttggctgc atccagaaca ctgccaagat aactaacatc gtgactgact
      361 gttgctggac ctgcaccagc gttgtttctc agcctgcaat gttcaattac gtcattttct
      421 gtcagttcag atagtttgat cgcggagatg tcactatcca taagcagttc tagcacatat
      481 cttttagtac ggtctgcttt acctccggca tttggtcatt taaatatttg tgtagtaagt
      541 cacggactgt aagtccgtca acagcatttg atgatgaaat gccatataga tctaatacca
      601 tcactttctg tgtgccccat gttttagcat gagcatgaac gcggcggcta tctcacgggt
      661 gatcatccct ttggcatact gaaaagtatc gggtgttact gaaccgtgtt gatacatcaa
      721 ctgcactgga tattgagtgg ccgataatac cgacgttata acctataaat gccaatcagg
      781 ctgaatgttg aaaggataga atgaacagcc cgacacacaa aaaacggagt ccggctccgg
      841 tttttgtgtt gccatataag gcagatgttt gttacagcta tttaagtctg gagttcaaat
      901 taaaataggg agttttgt
//
LOCUS       DAZKGS010000504          916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKGS010000504 DAZKGS010000000
VERSION     DAZKGS010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>916
                     /locus_tag="ACHOQY_005415"
     CDS             <1..>916
                     /locus_tag="ACHOQY_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9681268.1"
                     /translation="TVNLIKSARVGYTKMLLGVVGYFIEHKSRNSLLFQPTDSAAEDF
                     MKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSV
                     DVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGTCQIEKAANES
                     AHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQ
                     SNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDA
                     LKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT          210 a          230 c          260 g          216 t
ORIGIN      
        1 gcactgttaa tctgattaaa tctgcccgtg ttggctatac aaagatgttg ctgggggtgg
       61 tcgggtattt tattgagcat aaatcccgaa acagtctgct ttttcagccc acggattctg
      121 ccgctgaaga ttttatgaag tctcacgtgg aggcgacgat tcggaacgtg ccatgcctga
      181 aagacctttc cccatggctg ggtcgtaaac atcgtgacaa tactctcacg ctgaaacgct
      241 tttcatcggg cgtcggtttc tggtgcctgg gcggcgctgc cgccaaaaac taccgtgaaa
      301 aatccgtgga cgtggtctgc tatgacgaac tttcctcgtt cgagccggat gtcgaaaaag
      361 agggctcgcc aaccctgctg ggggataagc gtattgaggg gtcggtgtgg ccaaaatcca
      421 ttcgcggctc gacgcctaaa atcaaaggca cctgccagat cgaaaaagcc gctaacgagt
      481 cggcgcattt tatgcgtttt tatgtgccct gcccgcactg tggggaggag cagtatctga
      541 aatttggcga tgagtccacg ccttttgggc ttaaatggga gaaggacagc cctgaaagtg
      601 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgaactg gaccagagca
      661 acgggcggtg gatctgtgaa aacacgggca tgtggacccg tgacggtctg acgtttttca
      721 gcgcccgggg tgatgaaatt ccgccgccgc gctccatcac gttccatatc tggacggcgt
      781 acagtccgtt caccacctgg gtacagattg tctatgactg gctggatgca ctgaaagatc
      841 ccaacggcct gaaaaccttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
      901 gcgaaaaact cgatca
//
LOCUS       DAZKGS010000505          895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKGS010000505 DAZKGS010000000
VERSION     DAZKGS010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..406
                     /locus_tag="ACHOQY_005416"
     CDS             <1..406
                     /locus_tag="ACHOQY_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9681269.1"
                     /translation="QCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPP
                     SAVMSPGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKK
                     SAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            403..>895
                     /locus_tag="ACHOQY_005417"
     CDS             403..>895
                     /locus_tag="ACHOQY_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9681270.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTT"
BASE COUNT          209 a          234 c          277 g          175 t
ORIGIN      
        1 acaatgcgag acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagca
       61 ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg aaaacaatcc
      121 ggagatgcca ccgtccgcgg tgatgtctcc tggtattgaa tatatggagg atggtcttcc
      181 cgatccggtg aaagcgatgg ggcgtcttct ggtggagaac attaataccg accccaggct
      241 ggcgctggat gcggcttata agctggcgca gttcacgcac cataaaaaag gggatgccgg
      301 taaaaaatcg gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg cggtgccacc
      361 accaccccgc ctggtggtga ataatgataa tgagggcaac ggatgatacc tgtgtggagc
      421 acggcctgcc cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggctccg
      481 atttatccgg accaggctgc acatgcactg gcgattttta aacaactgcg aattgtggat
      541 gcaccgggta gcccgacatt cggggagtcc tgtgcaccgt gggtgtttga cctggtggcg
      601 gccctgtttg gctcctacga tgcgcagacc ggtgttcgcc atatcaagga agtgtttatc
      661 cttatcccca agaaaaactc gaagtccacg ctggctgcgg ggatcatgat gacggcgctg
      721 ttactgaact ggcggcaggc ggcgggctac accattctgg ccccgaccgt ggaggtggcg
      781 gctaacgcct tcaaccctgc cagggatatg gtacgacggg acgatgatct ggatgacctc
      841 tgtcaggtgc agacccatat ccggaccatc acccatcggg tgacggacac caccc
//
LOCUS       DAZKGS010000506          895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKGS010000506 DAZKGS010000000
VERSION     DAZKGS010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..153)
                     /locus_tag="ACHOQY_005418"
     CDS             complement(<1..153)
                     /locus_tag="ACHOQY_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9681271.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>895)
                     /locus_tag="ACHOQY_005419"
     CDS             complement(143..>895)
                     /locus_tag="ACHOQY_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681272.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          207 a          270 c          217 g          201 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcact
//
LOCUS       DAZKGS010000507          893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKGS010000507 DAZKGS010000000
VERSION     DAZKGS010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>893
                     /locus_tag="ACHOQY_005420"
     CDS             <1..>893
                     /locus_tag="ACHOQY_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9681273.1"
                     /translation="ISQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSA
                     FVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNE
                     ITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIGAGIEDT
                     DGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPP
                     AFSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGK
                     AFPRNNSYASGTVTVTVYDDQG"
BASE COUNT          241 a          211 c          266 g          175 t
ORIGIN      
        1 atatctcagg tggaaacctc tgcccgttat ctggggaccg gcagtcagtg gagtgtatcc
       61 ggcccgcaca ttaagcccgg gaaggatttc tggttttacg tgcgcagcgt caacctggtg
      121 gggaaatctg cgtttgtgga agtcagcggg cagcccagca atgatggtga agggtatctg
      181 gaatttttcc gggaaaaaat aggaaaactg catctggctc aggggctatg ggagctgata
      241 gacaacagcc agcttgcgga tgagatggcg gagatgaaga ccaccatcac cgaaacccgc
      301 aatgaaatca cacagacggt cagtaaaacg ctggaagacc agagcgccac cattcagcag
      361 atacagcgcg tgcagaagga cacaaatgat gacctggctg cgctgtacat gctgaaggtt
      421 caaaaaacga aagacggcat tccctatgtg gccgggattg gtgcagggat tgaggatact
      481 gatggccagc cactgagcaa catactgctg ctggctgacc gtattgcgat gataaatccg
      541 gagagcggca acagcacgcc gttatttgtg gcgcagggga atcagttgtt catgaacgat
      601 gtgttcctga agcgactgtt tgcggtgagt atcacctcgt ccggcaatcc cccggcattt
      661 tccctgacgc cggaagggaa gctgacagcc aggaacgcgg atatcagtgg agcaattacc
      721 gcgaataccg gcacgctcaa taatgtcacc attaacgaga actgtgtcat cagagggaaa
      781 ctgtctgcaa accagataga aggcgacctg gtgaagacgg tggggaaagc ctttccccgg
      841 aataacagtt atgccagcgg gacggtaacc gtcacagttt acgatgacca ggg
//
LOCUS       DAZKGS010000508          889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKGS010000508 DAZKGS010000000
VERSION     DAZKGS010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>889
                     /locus_tag="ACHOQY_005421"
     CDS             <1..>889
                     /locus_tag="ACHOQY_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9681274.1"
                     /translation="VYDRSGKQVRCFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAI"
BASE COUNT          225 a          248 c          272 g          144 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg ctgctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatc
//
LOCUS       DAZKGS010000509          883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKGS010000509 DAZKGS010000000
VERSION     DAZKGS010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>883
                     /locus_tag="ACHOQY_005422"
     rRNA            <1..>883
                     /locus_tag="ACHOQY_005422"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          245 a          195 c          276 g          167 t
ORIGIN      
        1 ggaaaggcgc gcgatacagg gtgacagccc cgtacacaaa aatgcacatg ctgtgagctc
       61 gatgagtagg gcgggacacg tggtatcctg tctgaatatg gggggaccat cctccaaggc
      121 taaatactcc tgactgaccg atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc
      181 cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta caagcagtgg gagcacgctt
      241 aggcgtgtga ctgcgtacct tttgtataat gggtcagcga cttatattct gtagcaaggt
      301 taaccgaata ggggagccga agggaaaccg agtcttaact gggcgttaag ttgcagggta
      361 tagacccgaa acccggtgat ctagccatgg gcaggttgaa ggttgggtaa cactaactgg
      421 aggaccgaac cgactaatgt tgaaaaatta gcggatgact tgtggctggg ggtgaaaggc
      481 caatcaaacc gggagatagc tggttctccc cgaaagctat ttaggtagcg cctcgtgaat
      541 tcatctccgg gggtagagca ctgtttcggc aagggggtca tcccgactta ccaacccgat
      601 gcaaactgcg aataccggag aatgttatca cgggagacac acggcgggtg ctaacgtccg
      661 tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg gttaagtggg
      721 aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc atcatttaaa
      781 gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg gggctaaacc
      841 atgcaccgaa gctgcggcag cgacgctgat gcgttgttgg gta
//
LOCUS       DAZKGS010000510          881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKGS010000510 DAZKGS010000000
VERSION     DAZKGS010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..269
                     /locus_tag="ACHOQY_005423"
     CDS             3..269
                     /locus_tag="ACHOQY_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP9681275.1"
                     /translation="MIITIALISLLSACTRENSLKENQNKRSGESQAIVDARQFFSEH
                     PEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(330..881)
                     /locus_tag="ACHOQY_005424"
                     /pseudo
     CDS             complement(330..881)
                     /locus_tag="ACHOQY_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          272 a          170 c          142 g          297 t
ORIGIN      
        1 acatcataat aaccatcgcg ctgatatcgt tgctctctgc atgcaccaga gaaaattcat
       61 taaaggagaa tcaaaataaa agaagtggcg aatcccaagc gatagtagat gcaagacagt
      121 tctttagcga acacccggaa tatatatcat cagcagaact ggagcaaaag ctatatcgcg
      181 aatttgcaca ggttactaca gttccagctt ataaagagat gagtatgtat caattgctta
      241 tcatttcaca agatcgttta actcattaaa taaggatcta cacgcttttt gcgtgtagat
      301 ccttataaat ttctgactac gacagatgtt taatttttta aattatcgtc tttattctcg
      361 atttttgtgt cttcaccctc aatgaattta tcttctgaag agagctgttc atcttgaaca
      421 ggttgcccag cattctccac atcttcaagc cacagcagaa gacgtaatat ttcatctata
      481 ttttcgaagc taacataatc gtagcgacga tgggtagcat aattttttcg tgctaatttt
      541 ttatctgtga taattggtat tccgatttcc tttgcatatt tccttacgcc taaggccacc
      601 tcattagtta ctcttacaga aatcaatgga ataggtgaaa gatgtggctt aaagtatatc
      661 cctattgcaa tgtgagtagg gttggaaatc atcaaacgac tattactgac atcagacttc
      721 aattgctcag aaagaatttc ctgatgcgct ctctgcgttt agacttaatt tctggatttc
      781 cttcttgctc cttgtattct cttttaactt cttgtttatc cattttcata tctttcataa
      841 ataaaaaata ttcagcgata aaatcaaaaa ttaagacaat t
//
LOCUS       DAZKGS010000511          878 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKGS010000511 DAZKGS010000000
VERSION     DAZKGS010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..593)
                     /locus_tag="ACHOQY_005425"
     CDS             complement(<1..593)
                     /locus_tag="ACHOQY_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9681276.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEHLAQQNLAAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRS"
     gene            complement(595..873)
                     /locus_tag="ACHOQY_005426"
     CDS             complement(595..873)
                     /locus_tag="ACHOQY_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681277.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
BASE COUNT          193 a          238 c          252 g          195 t
ORIGIN      
        1 attgagcgga ttttttccgc acgcaacccc agcgaacgac gtaataccgc ttccggtagc
       61 gcgtccgcca cctgattgcg gaccgcccac caggataatt cagccagaga taattcacgc
      121 tcctgcgagc cattcattgc atggcgtatg acgtcaatca tccaggctgc caggttttgt
      181 tgagccagat gttccagtga ttcggaggtc tggtcgcgca gctggttgtc gcagtgccag
      241 cacaacacca ttgcgccggt accataacgg tgaatgacgg tttcactgtg gtgataatca
      301 ccgtgtggcc actggcagga tttaacatgg cgcagtaacc agtcagacaa tgcgccagcg
      361 ccaccagcag cacgaatcac tcgttcgtcg ctgaaaaatg gcagtaatga tttatcctcc
      421 gccagcggct ggcgaacggc aggaacgacc ccggacggca gattacgcat gcttttcggt
      481 tccggctcca ccagtacccg ggtattgtgg aataccggca tggattcacg gcccggctta
      541 acgatcacca gcccgagttc cggtaccaga acaggtcgaa gtaatacccg cacgttacct
      601 ccagatgcgt tgctggaatg tgcgggacgg acgcggtggt cgttcggagt aaggaagcct
      661 gacggagatt atccagtgac gataatcgag gctgagggct ttcttaatct cgtatccgtg
      721 tctgcggtag cactgaatta gccactcggc ctgttcttca gtgcatgggg ggtgctggaa
      781 ccagtcagat ttgaaagtgc gggaacgccg cccgtgcctg ctggcagggg cggcagagtt
      841 atccgaattg taaaatttgg tatcgtgcgc catctgtt
//
LOCUS       DAZKGS010000512          876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKGS010000512 DAZKGS010000000
VERSION     DAZKGS010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..214
                     /locus_tag="ACHOQY_005427"
     CDS             <1..214
                     /locus_tag="ACHOQY_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9681278.1"
                     /translation="HKQCVVCNQHKSGNLVPYRVELINRIGQEAVDEIESNHNRHRWT
                     IEECKAIKAGYQQKLKDLRNSRSEAA"
     gene            211..405
                     /locus_tag="ACHOQY_005428"
     CDS             211..405
                     /locus_tag="ACHOQY_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9681279.1"
                     /translation="MTFTVKTIPDMLLEAYGNQSEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            398..832
                     /locus_tag="ACHOQY_005429"
     CDS             398..832
                     /locus_tag="ACHOQY_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9681280.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
BASE COUNT          252 a          184 c          248 g          192 t
ORIGIN      
        1 tcacaagcaa tgcgtggtgt gcaaccaaca taaaagcgga aatctcgttc cgtatcgcgt
       61 cgaactgatt aaccgcatcg ggcaggaagc agtagacgaa atcgaatcaa accataaccg
      121 ccatcgctgg actatcgaag agtgcaaggc gatcaaggca gggtaccaac agaaactcaa
      181 agacctgcga aacagcagaa gtgaggccgc atgacgttca ccgtaaaaac cattcctgac
      241 atgctccttg aggcatatgg aaatcagtcc gaggtagccc gaatactgaa ctgcaatcgt
      301 gccacagtca gaaaatacat tggcgataaa gaagggaaaa agcacgccgt cgtcaatggc
      361 gtccttatgg ttcatcgcgg atggggtaaa gatactgatg cgtgatatcc ggcaggttct
      421 tgagcgctgg ggggcatggg cggcaaataa ccatgaggat gttacatggt cgcccattgc
      481 tgccggattt aagggactga tccccgaaaa agtaaaatca cgtccacagt gttgtgacga
      541 tgacgcgatg attatatgcg ggtgtatggc tcgccttaac aggaacaaca gcgatctgca
      601 tgacttgctg gttgattatt acgtgttggg ggagacgttc atggcgctgg cacggaaaca
      661 tgggtgctct gacacctgta taggtaaacg ccttcacaaa gcggagggga ttgttgaagg
      721 catgctgatg atgctgggag tgaggcttga gatggatcgg tatgttgagc gtgaattgcc
      781 gggagggaga acctctgtat tttatcagcg aaaaaatagt ttacgatcgt aaaaatctgc
      841 atatcatgat aagagtggtt acattgccac gctgct
//
LOCUS       DAZKGS010000513          869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKGS010000513 DAZKGS010000000
VERSION     DAZKGS010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..184)
                     /locus_tag="ACHOQY_005430"
     CDS             complement(<1..184)
                     /locus_tag="ACHOQY_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9681281.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHOQY_005431"
     CDS             complement(184..399)
                     /locus_tag="ACHOQY_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9681282.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..>869
                     /locus_tag="ACHOQY_005432"
     CDS             542..>869
                     /locus_tag="ACHOQY_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681283.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNN"
BASE COUNT          244 a          232 c          170 g          223 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataaca
//
LOCUS       DAZKGS010000514          861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKGS010000514 DAZKGS010000000
VERSION     DAZKGS010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..177
                     /locus_tag="ACHOQY_005433"
     CDS             <1..177
                     /locus_tag="ACHOQY_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681284.1"
                     /translation="MQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAV
                     AVITGVNYGSASGG"
     gene            222..443
                     /locus_tag="ACHOQY_005434"
     CDS             222..443
                     /locus_tag="ACHOQY_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681285.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            389..>861
                     /locus_tag="ACHOQY_005435"
     CDS             389..>861
                     /locus_tag="ACHOQY_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681286.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRIT"
BASE COUNT          183 a          221 c          286 g          171 t
ORIGIN      
        1 atgcagtctg agccgaccgg cgacagtacc acgccgtccc cggtggagct ggtttccatg
       61 ttccagacag gcagcgtggc catccgtgcg gagcgctgga tcaactggcg tcgtcgccgt
      121 accgcggcgg tggcggtgat caccggagtg aactacggca gtgcgtccgg cggctgagtc
      181 tgatgaggag gacgggaggc gtgcgcctcc cgtaacaggt tatggcaaag atccgatatc
      241 tgcagggcac gcatgatgcc cgggccgggg atatccgtga tgtggcacag ccgtgtgcgg
      301 aggtgctggt tcgcctgggg aaggcggagt acatcacagt gcgacgtccg gcaggtcaga
      361 aaaagaaacg tgatgcggag catggcgaat gtggaacctt ttgcggcgaa cccgaaaaaa
      421 ccagaaatca ggacgtgatg taaaagaggt gggctggacc agcctgtttc aggcggtggc
      481 tgagcctttt gccggcgcct ggcagcaggg cgtgaaagcc gatccggaaa gtgtcctctc
      541 ctttcatgcg gtgttttctt gcatttcgct gatatcccag gatatcgcca aaatgcgact
      601 gcgcctgatg cagaccgata cacaggggat ccgccgtgaa aaacggcagg gggatattgc
      661 ccgtctctgt cgtcgtccca atgcacagca gaatcgtatc cagttttttg aactgtggct
      721 gaacgccaaa ctgcgtcacg gcaatacggt ggtgctgaaa atccgtaact cccgggggca
      781 gatcaaagaa ctgcgtattc tggactggag ccgggttgaa cctctggtgg cggatgacgg
      841 cgaggtgttc tatcgcatta c
//
LOCUS       DAZKGS010000515          859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKGS010000515 DAZKGS010000000
VERSION     DAZKGS010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..460
                     /locus_tag="ACHOQY_005436"
     CDS             <1..460
                     /locus_tag="ACHOQY_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681287.1"
                     /translation="WFESGDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVK
                     VTNVGKPSVAEERSEITPVTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSA
                     DPSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            474..>859
                     /locus_tag="ACHOQY_005437"
     CDS             474..>859
                     /locus_tag="ACHOQY_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9681288.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNAL"
BASE COUNT          212 a          199 c          269 g          179 t
ORIGIN      
        1 ctggtttgaa agcggggatg tgcgggccta taaaatccgt ttcccgaacg gcacggtgga
       61 tgtgttccgt ggctgggtca gcagtatcgg taaggccgta acggcgaagg aagtgatcac
      121 ccgtacggtg aaagtgacca acgtgggtaa accttctgtg gcggaagaac gcagcgaaat
      181 tacgccggtc actgcgatta aggtgacgcc gacatctggt acggtggcaa aagggaaaac
      241 aacaaccctg acggtttctt ttgagccgga aagtgcaacc gacaaaacgt tcagagcggt
      301 ttccgccgat ccgtcaacgg gaaccattgc tgtgaaagat atggcgatca ctgtgacggg
      361 ggttaaggct ggaaaagtga gtatccccgt gatttccggt aatggtcagt ttgccacggt
      421 agctgaagtc accgttactg aagcgggcgc tgcagggtaa acggaggtaa tacatgtttc
      481 tgaaaaccga acaatttgaa tataacggtg tgtccgtcac gctttccgag ctgtctgcgc
      541 tgcagcgtat cgagcatctt gccctgctga aacgacgggc agaacaggct gaagccagcg
      601 gtaacctgca ggtgagcgtg gaagaccttg tcagaaccgg cgcgtttctg gtggcgatgt
      661 ccctgtggca taaccatcca cagaaaacgg agtcaccatc aatgaatgag gctgtgatgc
      721 agatcgaaca ggaggtgctc accacctggc ctgctgatgc cattgcccgg gcggaagacg
      781 tggtgttgcg tctgtccggg atgagcgggg ctgttcatgt ggatacggat atcaccgaag
      841 tggcgaaaaa taacgcgct
//
LOCUS       DAZKGS010000516          859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKGS010000516 DAZKGS010000000
VERSION     DAZKGS010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..147
                     /locus_tag="ACHOQY_005438"
     CDS             <1..147
                     /locus_tag="ACHOQY_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681289.1"
                     /translation="SRANTWDAVGAYNAGYFNTPNAVELRRQYAMKIYKTYTKLKNNE
                     QIID"
     gene            complement(144..>859)
                     /gene="escU"
                     /locus_tag="ACHOQY_005439"
     CDS             complement(144..>859)
                     /gene="escU"
                     /locus_tag="ACHOQY_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP9681290.1"
                     /translation="FAVEKIKPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLI
                     FYFMGHSYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGL
                     KKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAI
                     CLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLARSLYKNIHKGQYIT
                     EDFFEPVAQLIRIAIDLDY"
BASE COUNT          286 a          161 c          140 g          272 t
ORIGIN      
        1 tccagggcga atacatggga tgcagttggt gcgtataatg caggctactt taacacacca
       61 aatgcagtag aactcagaag acaatatgca atgaaaatat ataaaacata cactaaatta
      121 aaaaacaatg aacaaattat cgattaataa tcaagatcta ttgctatacg aatcaattgt
      181 gctacaggtt caaaaaagtc ttctgttatg tattgtcctt tatgtatgtt cttataaaga
      241 cttcttgcca aagtaatatc ttcaatcact ggaatatcat agagttcagc cagtttaatg
      301 atctgtagag ctttggcatc ttttcctgtt tcaataacta aaggcaatgg agtctccccg
      361 agtttatagt ataggcaaat cgcaatgtga gtcgggtttt tgacgatcac ggttgatttt
      421 ttgatgttat tagccagact tccactttgt atctcagaat gaaggcgacg gcgctcccct
      481 ttaatttcag ggttaccatc agtatccttg gcttctcgtt tcacctcatc tttactcatt
      541 ttcattttct tcagtccctc atgcttctgg aaccagaaat caaatactga aaagagtaga
      601 tatccgaata acacgccttt ccataaaaga aaaacaaaaa aggcaacaac gacaagagtt
      661 tgatatgcgt tcagtcttgt gaaatttgca aactcatttg catatgaatg ccccataaag
      721 taaaaaatga gagtaattat cactaactta aatactgact taagcagctc aaaaatactc
      781 tttacagaaa aaatattttt caggttattt tttacactaa tcttctgagc cgatggtttt
      841 attttttcaa ccgcaaaga
//
LOCUS       DAZKGS010000517          858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKGS010000517 DAZKGS010000000
VERSION     DAZKGS010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..595
                     /locus_tag="ACHOQY_005440"
     CDS             <1..595
                     /locus_tag="ACHOQY_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9681291.1"
                     /translation="NHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSI
                     ADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNG
                     QSGEKFLQELCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAA
                     MSTLLGDRVMDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            621..>858
                     /locus_tag="ACHOQY_005441"
     CDS             621..>858
                     /locus_tag="ACHOQY_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681292.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVA"
BASE COUNT          223 a          184 c          266 g          185 t
ORIGIN      
        1 gaatcatcag gtgcgggttg aaaaaatcct gaaccgtgcg ggcatccagc cgcttcacag
       61 gaagtgctca ttcgggaact accgggtgca gaacgacggt cagcgccatg ctctgagcca
      121 ggcgaaatcc attgccgatg aattgatgac cggatgtaca aacttcgtgt tcagcggtaa
      181 acctggtacc ggtaaaaatc acctggcagc agcgattggc aatcggctga tggcgaaggg
      241 gagaagcgtg attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg acggctacga
      301 caacggccag tccggggaaa aatttttaca ggagctttgt ggagttgacc ttctggtcct
      361 tgatgaaatt ggcatgcagc gggatacgcg caacgagcag gtcacgctga accagatagt
      421 cgaccgcaga acggcttcga tgcgtagtgt cggaatgctg acgaacctga atcacgcagc
      481 gatgagcaca ctcctcggag atcgggtgat ggaccgtatg accatgaatg gtggtcgttg
      541 ggtgaatttt aactgggaga gctggcggtc aaacgttgga cgtcagggta tgtgagaatt
      601 tttgacgagg taaattttcg atggaaactg tattgcatgc actgaaagcg atgggaaaag
      661 ccaattctgt tgaactggcg gcgcggcttg atatcagccg tgaagaagtt cttaacgaac
      721 tgtgggaact caaaaaaaat ggcgttgttg ataaaacggg tcacacctgg tttctggctg
      781 tcgaaggtga agccggggta accgaagggc aggcactaca acctgaagcg ccggatgtgg
      841 taaccgaaga ggtcgctc
//
LOCUS       DAZKGS010000518          853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKGS010000518 DAZKGS010000000
VERSION     DAZKGS010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(393..>853)
                     /locus_tag="ACHOQY_005442"
     CDS             complement(393..>853)
                     /locus_tag="ACHOQY_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9681293.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          206 a          226 c          228 g          193 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ccataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgt
//
LOCUS       DAZKGS010000519          852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKGS010000519 DAZKGS010000000
VERSION     DAZKGS010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..504)
                     /gene="yhaB"
                     /locus_tag="ACHOQY_005443"
     CDS             complement(<1..504)
                     /gene="yhaB"
                     /locus_tag="ACHOQY_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP9681294.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILF"
     gene            complement(760..>852)
                     /locus_tag="ACHOQY_005444"
     CDS             complement(760..>852)
                     /locus_tag="ACHOQY_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP9681295.1"
                     /translation="NTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          251 a          161 c          165 g          275 t
ORIGIN      
        1 aaagagaatt tgccaacaat ctgaagtaat gctggtttta ttaaagtgca caaggggtgt
       61 taagatggct tcttttcggg tgaattttgc acaatactta gaaatcgtct ccttaataca
      121 ttccagctct tttttattga cggctgagca agtacattgt tcgccaaaag gtgcaaaagt
      181 gcctacatct aatgtttgta tgatataatc atcatgttcc cctgtagaaa tatctatgat
      241 gaatggactc atattttccg gttggattcg gatcattaca gaaggaacat cgccttgata
      301 gaaattcaat gcagtaattg cagataatac tctccgcagt tcattactta tactgtaaat
      361 tttattaacg tcattggtgg agacaatttc tgcaaacttt ctttttaccg tccaataatc
      421 aatatttcta ttgtgattgt taaccactgc gtgcattggc gggattgaag ggtgaaaaat
      481 atgtgcaatt gggaagcctt tcattgatgc ctgccttgtc gtcatgacaa acaataatag
      541 atgcttattt tcctcgcagg gttctttcgc actatcaaaa aacgctcatt tacccggcgt
      601 tacagtaaac gaagcaacat tctgtggcct acgacgatca aaagctctgt gaaaatttcc
      661 cttgttgtct ttgtctgacc ttatcgcggc gaccagcaat tattcgtttt gtgatcctca
      721 acacaaaatg ctattcgtcg cctatgagtg gagaagaatt tatggaaaac gattgcattc
      781 acaaaatgac gtcctgtcag aagagaaaaa gttgacgtta atcaattgtt cgagatgatc
      841 aatacctgta tt
//
LOCUS       DAZKGS010000520          842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKGS010000520 DAZKGS010000000
VERSION     DAZKGS010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..284
                     /locus_tag="ACHOQY_005445"
     CDS             6..284
                     /locus_tag="ACHOQY_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681296.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            286..>842
                     /locus_tag="ACHOQY_005446"
     CDS             286..>842
                     /locus_tag="ACHOQY_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9681297.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          187 a          225 c          228 g          202 t
ORIGIN      
        1 aaccgatggc gcacgatacc aaattataca attctgatga ttctgccgtc tttgccagca
       61 ggcgcggacg gtgttttcac gcattcaaat ctgactggta ccagcatccc ccatgcactg
      121 aagaacaggc cgaatggctc attcagtgtt accgcaggcg cggatgcgag gttaaaaaag
      181 cccttagcct cgactaccgt cactggataa tctccgtcag gctcccttac tccgaacggc
      241 caccgcgtcc gtcccgcaca ttccagcaac ggatctggag gtaatgtgcg ggtattactt
      301 cgacctgttc tggtaccgga actcggtctg gttatcgtta agccaggccg tgaatccatg
      361 cgggtatttc atggcggcag ggtgctggtg gagtctgaac cgaaaagcat gcgtaatctg
      421 ccgtccgggg tcgttcctgc cgttcgccag ccgctggcgg aagataaatc attactgcca
      481 tttttcagca atgagcgtgt gattcgtgct gctggtggcg ctggtgcact gtctgactgg
      541 ttattacgtc acgttaaatc ctgccagtgg cctcatggtg attatcatca cagcgaaact
      601 gtcatacatc gttacggtac cggcgcgatg gtgttgtgct ggcactgcga caaccagctg
      661 cgcgaccaga catccgaatc acttgagcaa cttgctcaac aaaacctgtc agcatggatg
      721 attgacgtca tccgccacgc aatgaatggc atacaggaac gggaattatc gctggctgaa
      781 ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc agtatcgcgt
      841 cg
//
LOCUS       DAZKGS010000521          842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKGS010000521 DAZKGS010000000
VERSION     DAZKGS010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..456
                     /locus_tag="ACHOQY_005447"
     CDS             <1..456
                     /locus_tag="ACHOQY_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9681298.1"
                     /translation="LVDRWRELEGATAKIPQTFSEALRLAADLEDQKAELEKQLALAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGARRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            456..>842
                     /locus_tag="ACHOQY_005448"
     CDS             456..>842
                     /locus_tag="ACHOQY_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9681299.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDKIKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDER"
BASE COUNT          268 a          195 c          217 g          162 t
ORIGIN      
        1 cttgttgacc gctggcgaga actcgaaggg gcaaccgcga aaataccaca aaccttttct
       61 gaggcattgc gccttgcggc cgaccttgaa gaccagaagg ctgaactgga gaaacagctt
      121 gctctcgcag cacctaaagt tgagtttgcc gatcgagttg gcgaggccag cggaattttg
      181 attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc gtggatgcgc
      241 gatcacaaaa tccttattgc ttcaggtgcc cggcgcaatg tgccaatgca ggaatatatg
      301 gatcgcggct atttcacagt gaaagaaaca gcggtcaata caaatcacgg aatacagata
      361 tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgacaagaaa gctgctagat
      421 aacggaatgc ttaaagtaac aggggaggct gcttaatggc taaaccagcg cgaaggaaat
      481 gcaaaatctg taaggaatgg tttcacccgg cattctcaaa tcagtggtgg tgcagcccgg
      541 aacacggaac taaattagcg ctcgaacgac gaaataaaga acgcgaaaag gcggaaaaaa
      601 cagcagagaa gaaacgacga cgagaggagc agaaacagaa agataaaatt aagattcgaa
      661 aactcgcctt aaagccccgc agttactgga ttaaacaagc ccaacaagcc gtaaacgcct
      721 tcatcagaga aagagaccgc gacttaccat gtatctcgtg cggaacgctc acgtctgctc
      781 agtgggatgc cggacattac cggacaactg ctgcggcacc tcaactccga tttgatgaac
      841 gc
//
LOCUS       DAZKGS010000522          833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKGS010000522 DAZKGS010000000
VERSION     DAZKGS010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..756)
                     /locus_tag="ACHOQY_005449"
     CDS             complement(58..756)
                     /locus_tag="ACHOQY_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9681300.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRSDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(756..>833)
                     /locus_tag="ACHOQY_005450"
     CDS             complement(756..>833)
                     /locus_tag="ACHOQY_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681301.1"
                     /translation="VCREWSVTDNARYSDFSCTIEQVVN"
BASE COUNT          177 a          291 c          193 g          172 t
ORIGIN      
        1 caggcgcaca ccgccgggca tgcgccagaa tcgctgattc tgtctgtgtc attggattta
       61 ctgcgaaagt ttgttaatgg aaaggaaacc gccaaaatta gccaccatgc cgcgcatctc
      121 acacccgcgc atgcacttgc tgcatctgtc cttacggata tccgtggtgg ggttgtcgaa
      181 ctcatccgcc actgccggac cgttataccc gcattcatca gagcggtaat cccacataca
      241 ggtgttcgcc agcatgatgc gaccgggaaa cagcgccccg tccgtctcgg tcggtgtggc
      301 cagcacaaac gaggccgtca tggccgtcag cgatgacatc tgctccacca cccaccggtc
      361 agtcagctcc tgctccgggt cggcctccgg attgcctgcc acaaagttca ccgcatccag
      421 aaaacgcgca tacacccggc ggcggaccac cgtggcaccc accaggctct gcaaatcctc
      481 cgccatcccg gtgacaagac cgaacagatt ggacaccgtc agcgacgggc gggcactgct
      541 gccctttccg ttcatctcaa agccactgcc ctcaatcggg tacgcctcat acttacgccc
      601 ctgccaggtc accggctccc ttttttcatt cagctcattg cagaaaaaat accgctcacc
      661 gccctgcacc gtcaggtcga tttcccagag caccacccgc ggtgactgct ctgacttaac
      721 cgactcgttc aggctttctt cgtgaatatc ctgcatcagt tcaccacctg ctctatcgtg
      781 caactgaaat cactgtaccg ggcattatcc gtgacactcc actcacggca cac
//
LOCUS       DAZKGS010000523          833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKGS010000523 DAZKGS010000000
VERSION     DAZKGS010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..602
                     /locus_tag="ACHOQY_005451"
     CDS             24..602
                     /locus_tag="ACHOQY_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9681302.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          196 a          203 c          266 g          168 t
ORIGIN      
        1 ttggccgccg cctcagcctg tacgtgaaca cggcagcgga agccatccgt gccctgtcgc
       61 tgcagatgcc gggattccgc cgtcagatga acgaaggctg gtaccagata cgtattcgcg
      121 gtgaggacac ggcaccggag gcggtgtacg cccgtcttca cgaacctctg ggtgaggggg
      181 cggtcatcca tattgtgccg cgactggccg gagccggaaa gggcggactg cagattgtgc
      241 tgggggcagc agccatcgtg ggctctttct tcaccgccgg cgcaacgatg gcgttgtggg
      301 gcgcagccct gagtgccgga gggctgactg ccaccacgat gctgttctca ctgggtgcca
      361 gcatgatact gggcggtgtg gcccagatgc tggcaccgaa ggcaaaaaca ccggattacc
      421 gcgcaacgga taacggtaaa cagaacacgt atttttcgtc actggataac atgattgccc
      481 aggggaaccc gatgccggtg ccttatggtg aaatgctggt tggttcacga cggatatccc
      541 aggacatcag cacccgtgat gagggcggag acgggaaagt ggtggttatc gggcggggat
      601 gaaaataaaa aaatcccgca gagttagcgg agctgcggga gagaacgatg aagattaacg
      661 ttatggagtt atttttcagg catcaaaaaa gtaatgcagc gtcattattg cggctacagg
      721 caattgccgg aaatgtgaag agtttcagaa attttattcc gtcatgacac aggcaccctc
      781 cggggtgcct gttgttttct ggcataaaca gattcagaca tcagacagga gag
//
LOCUS       DAZKGS010000524          822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKGS010000524 DAZKGS010000000
VERSION     DAZKGS010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..345
                     /gene="dicB"
                     /locus_tag="ACHOQY_005452"
     CDS             157..345
                     /gene="dicB"
                     /locus_tag="ACHOQY_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9681303.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            342..530
                     /locus_tag="ACHOQY_005453"
     CDS             342..530
                     /locus_tag="ACHOQY_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9681304.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            626..>822
                     /locus_tag="ACHOQY_005454"
     CDS             626..>822
                     /locus_tag="ACHOQY_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9681305.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPG"
BASE COUNT          241 a          167 c          189 g          225 t
ORIGIN      
        1 tgatgtggtg aatgcggctg agcgcacgcg gaacagttaa aaccaaaaac agtgttatgg
       61 gtggattctc tttatccggc gttaattgtt aactggttaa cgtcacctgg aggcaccagg
      121 cactgcatca caaaattcat tgttgaggac gcgataatgg aaacgttatt accaaacgtt
      181 aatacgtctg aaggttgttt tgatattggt gttctgctca gtaaccggga gtttactgaa
      241 gatgccatta atatgaggaa atatgagcct tatctgctca atgataattc catactttcc
      301 cgaattgctc ttcttgaact tggtattttc ggagaacgtc aatgacttca gcatttgcac
      361 tgatgatgac ggtttttctt ataacgggtg aatcacagaa tgtgattacc ggaatttatg
      421 caagtaaaga atcctgcctc caggcaagag acgagcaaaa aatttctggt gaatgcctcc
      481 cgctaaaaaa agtatcgctg tacctgaata acgaaacacc ggctggataa tcctccagcc
      541 atattaacac cataccaacg gattaaaaat gccagcaatg gcagggattc gttcaccctg
      601 aaatctgtaa tgaggttaaa acaaaatgag taaggtcttt atttgcgctg ctattcctga
      661 tgaacaggcc ataaaagaag atagcgctgt tgcggtggcc actgccattg aagctggtga
      721 tgagcgtcgc gcacgcgcaa aatttcactg gcaatttctg gagcaattcc ctgcagctca
      781 ggactgcgct tataaattta ttgtctgtga ggataaaccc gg
//
LOCUS       DAZKGS010000525          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKGS010000525 DAZKGS010000000
VERSION     DAZKGS010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..642
                     /locus_tag="ACHOQY_005455"
     CDS             <1..642
                     /locus_tag="ACHOQY_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681306.1"
                     /translation="RLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLR
                     GRISGTQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASA
                     VSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEII
                     FAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            718..>811
                     /locus_tag="ACHOQY_005456"
     CDS             718..>811
                     /locus_tag="ACHOQY_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681307.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYI"
BASE COUNT          190 a          197 c          227 g          197 t
ORIGIN      
        1 cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa caaagtgatg
       61 aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc tgacatgatc
      121 aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc cgtggcatct
      181 tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt cagtccactg
      241 ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg aatgattgcc
      301 tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt caaaattctg
      361 gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac cggacatgag
      421 attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct ggagatcatt
      481 ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc actggtggtc
      541 agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg caatatggtt
      601 aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt ctgcgccttt
      661 tacgctttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt aaataccatg
      721 attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac gtggtctgcc
      781 ccactccggg cacaggatca acgttacatc a
//
LOCUS       DAZKGS010000526          810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKGS010000526 DAZKGS010000000
VERSION     DAZKGS010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..301
                     /locus_tag="ACHOQY_005457"
     CDS             83..301
                     /locus_tag="ACHOQY_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9681308.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            520..762
                     /gene="yiiF"
                     /locus_tag="ACHOQY_005458"
     CDS             520..762
                     /gene="yiiF"
                     /locus_tag="ACHOQY_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFP9681309.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT          255 a          129 c          200 g          226 t
ORIGIN      
        1 gcaaaaatcc gcttttttta aatgattttc cggcgtaatt ttcgcacata cattaatgag
       61 taatataggt aaggtggtgg taatggcgat gaatacggtt tttcttcatt tatcagaaga
      121 ggcaattaaa cggctgaaca aacttcgggg ctggcgtaag gtttcacgat ctgcaatttt
      181 acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta aagcaaaggg
      241 aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc ataaggaatg
      301 aaaaaaattt ttcattcttg aatataaaaa aacagatgcc cttattctgg tattaataca
      361 aggctgtttt acttgaactt ataataactg caactgttac atcgtatctg gaaaacgcct
      421 cgcaaaaata aaaaatgatg cgtaaatgag ctttctttaa agattcggca agggtacttt
      481 tctctccata gattcaaaaa tgatattggc gagatattta tgaactctct ggcaggtata
      541 gatatgggca gaattttact caatttatcg aatgaggtga ttaagcaact tgatgatctc
      601 gaggtgcagc gtaatcttcc tcgtgcggac ctattaaggg aagcggtaga tcaatacctg
      661 ataaatcaat cgcaaacagc aagaaccagt gttcctggca tctggcaagg gtgtgaggaa
      721 gatggtgtcg aatatcagcg taagctgcgc gaggaatggt aatgggaaaa aatgatgtca
      781 atcagattgc tgacaacgtg cgcgttgttc
//
LOCUS       DAZKGS010000527          807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKGS010000527 DAZKGS010000000
VERSION     DAZKGS010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..563)
                     /locus_tag="ACHOQY_005459"
     CDS             complement(<1..563)
                     /locus_tag="ACHOQY_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9681310.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHA"
     gene            complement(568..>807)
                     /locus_tag="ACHOQY_005460"
     CDS             complement(568..>807)
                     /locus_tag="ACHOQY_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9681311.1"
                     /translation="TPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPT
                     TDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          174 a          268 c          210 g          155 t
ORIGIN      
        1 gcatggttgg gtaccgatgc gccaaagcag cacagcagga tatcgcctgc ctgtgccgag
       61 gacaggggca cccggtaaaa accagtggcc tccatattgt ccagatacag gttctgacca
      121 ttgcgccacc actcatcctc gcggtgaaaa tccggcagcg ttatccccgc cagatggcag
      181 gcatccctga acagcgtgta acagtccgtc accccgtgtt caaaacgacg cccggtcagg
      241 tgcggcacac agcggaattt atgaatgtcc ccccggcaga ccagccacca gggcagggca
      301 ctttttatct gcagccgccg gtcggcctcg ctcagccagg gcaggccacc gggatggctg
      361 tggaccagtg ccacaatctc cccctgcatc tctgcccgca gccagtcttc cggggcaata
      421 cgaaaatatg cctcaggctc tgcagagata ttcacgcacg gctggtaccg gtcaccctcc
      481 ggcgtgctta tcacgaagcc gcaagactcc gcaggcgcac accgccgggc atgcgccaga
      541 atcgctgatt ctgtctctgt catggattta ctgcgaaagt ttattaatgg aaaggaaacc
      601 gccaaaatta gccaccatgc cgcgcatctc acacccgcgc atgcacttgc tgcatctgtc
      661 cttacggata tccgtggtgg ggttgtcgaa ctcatccgcc accgcaggac cgttataccc
      721 gcattcatct ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccaggaaa
      781 cagcgccccg tccgtctccg tcggtgt
//
LOCUS       DAZKGS010000528          798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKGS010000528 DAZKGS010000000
VERSION     DAZKGS010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..229)
                     /gene="ykfM"
                     /locus_tag="ACHOQY_005461"
     CDS             complement(<1..229)
                     /gene="ykfM"
                     /locus_tag="ACHOQY_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFP9681312.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIK"
     gene            complement(239..>798)
                     /locus_tag="ACHOQY_005462"
     CDS             complement(239..>798)
                     /locus_tag="ACHOQY_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681313.1"
                     /translation="TKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAI
                     GWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVAT
                     AIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKR
                     QQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          277 a          156 c          129 g          236 t
ORIGIN      
        1 attttattat cgtaagtaaa attacatgaa tatttaaaaa ggaaaacgac atgaaaccaa
       61 agcacagaat caacattctc caatcataaa atatttccgt ggagcatttt attattgaat
      121 atagaggttt aactccggta aaaaacaaag aagcattgaa tgcagggaaa aataatatgg
      181 ccataaaaaa catcgaaaga aactctttta atttaacatg taaacgcatg gttaatcctc
      241 atatcacggg tggagtgtta agaacataca taaatggagt catgttttcc cttttccatt
      301 tatcaagttc ctgttgccgt tttagtccat ctctaattgc atattttaat ttttctgata
      361 aatggcattg agcatcgatt tcatttaaaa caactgtaca cactatccct gtcagtaaaa
      421 ttgctcctcc gacaagaatt actggaaagc caaagctaac acaggcaaaa gaagccaggc
      481 ttccaatagc ggttgcaaca ccaccggcta taactgcttt ggccacatcc attgaaaaat
      541 cgccaagaaa attaaccaga tctcgctctg aactcataat aaattctatg gttctccagg
      601 ctactgaaaa ccagatacag aacttaactc cttttattga ttcagataaa gctcctcgcc
      661 agccaatagc gagttccaga acttgaggcg ctcgtgaaga gaaaatatat ttaccgagct
      721 tttctaaaag ccaggggctg gatgttgtga tttttatata tttctctcca agtatttcaa
      781 agtaacttgc ttttgtat
//
LOCUS       DAZKGS010000529          792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKGS010000529 DAZKGS010000000
VERSION     DAZKGS010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..792
                     /locus_tag="ACHOQY_005463"
                     /pseudo
     CDS             50..792
                     /locus_tag="ACHOQY_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594569.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          308 a          153 c          118 g          213 t
ORIGIN      
        1 atccaattta aacatcttag cataaaacaa atgatgaata aggaatttta tgtcaattga
       61 ctttacccca ggtataataa atacatatca cggcgatatt tataactgca caacaaatac
      121 cgataatgct aaaacgccag acacaccgaa gtggccttgc gataattggg aagaacaata
      181 acccattaat tccacattct ctggagaagg atatatctct gatcaatatg atttagcgca
      241 acaccaactt caacaaatta atgcgtgcca cacgaacact acatatacaa atgcagacta
      301 ctcaaaagtt gtggctcaac ttgtaagtct tattactaac attgaaacga taagttcgac
      361 acaacttacg caacagacac aatcaatact taatcagatt aataacatta gatatgaaaa
      421 aaacaaaagt gctgaatgtc gaatcattgt tgtcgctaat cctaaacccg acaaggcaat
      481 cataactaaa attagcgtag aagagggaat acccataaca ttcagtgtcc aaaccatgtt
      541 ttcagatact aattttattg ctgaacaacg agctgactta ccaactaata taaaagatat
      601 tcaaagcctg tatcaaaaaa tgacgaagtt atatattgag cacagtgaaa ataaaaacag
      661 gatgaaagtc tttgccggaa ccaactttat tgatttcaat atgacaggac agaacctgtc
      721 cgggttcgtt ctaaccttgt caaggtttta tttcgaagat ctacttaata taaatttcac
      781 tgacgaaaat tt
//
LOCUS       DAZKGS010000530          789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKGS010000530 DAZKGS010000000
VERSION     DAZKGS010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..98
                     /locus_tag="ACHOQY_005464"
     CDS             <1..98
                     /locus_tag="ACHOQY_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9681314.1"
                     /translation="RDVWRRQIDKRAAEKISASQSAGSKPEEPLI"
     gene            139..411
                     /locus_tag="ACHOQY_005465"
     CDS             139..411
                     /locus_tag="ACHOQY_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9681315.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            488..703
                     /locus_tag="ACHOQY_005466"
     CDS             488..703
                     /locus_tag="ACHOQY_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9681316.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            708..>789
                     /locus_tag="ACHOQY_005467"
     CDS             708..>789
                     /locus_tag="ACHOQY_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP9681317.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEI"
BASE COUNT          214 a          177 c          227 g          171 t
ORIGIN      
        1 tgcgtgacgt ctggcgcaga cagattgaca aaagggccgc tgagaaaatc agcgccagtc
       61 agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct taatcacatc
      121 tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga agcagtgaag
      181 gaagtcctgc gctctgaaga agtccggagc gcactgaaac aaaaacttcg ccataacctg
      241 gaagcgcgtc ttgatgcaga ggttgatgcc attctggatg agctgcttgg tgtacaggca
      301 gagccaccga ctgaagcggg agataccacc gcagagagcg gtgaagttca gcctgaatca
      361 ccggtcgccg atgcgactga acctcaaccc gaatcggtca tgatgctgta acggggagtc
      421 agggccatca gtagttaact ggtggctttt ttattgttgt cagcttccgg ataacgggag
      481 acggggtatg taccagatgg aaaaaatcac aacgggtgtg tcatacacca cgtcagcggt
      541 ggggacggga tactggttac tgcagctgct ggacaaagtc tctccgtccc agtgggtggc
      601 aatcggtgtg ctggggagtc tgctgtttgg cctgctgacg tatcttacta acctgtattt
      661 caaaatcaga gaggaccgtc gtaaggcggc gcggggaaag taaagcgatg aagaaaaaat
      721 acgaactggt tgttaaaggg ataaataatt acccggataa gattactgtt actgtggcac
      781 tggaaattg
//
LOCUS       DAZKGS010000531          787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKGS010000531 DAZKGS010000000
VERSION     DAZKGS010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          298 a          181 c          106 g          202 t
ORIGIN      
        1 tatggacagg atgacatttt tataaataac aaaaaccatt taatactgat acatcaataa
       61 aatacttctt ccctgctgac acagacaaaa tgcaccattt tttcttcaaa tacttctgta
      121 taatcgccag ccaatgatga gaatggatta aaataacact tcttcgacaa acacaaaaac
      181 agccttcatt ttacatcatc atccagtttc agttaacccc ggacgagcga cagtgtttca
      241 gtacaggaat taaaagccag cttttgctca gtaaaacaca ctcaactaat aatatctcag
      301 cagatattat cctgaccagg acacctcaca ttactgaatt ctcttccgac agtatcactg
      361 cacaaataaa aaagaacggg ataaatattc agaccggaag aatggtgtca caaaagcatt
      421 taatcattac gcatgaccag gaagggaaac acttaaccac atggtatcaa cagatatcat
      481 tacgtgtgca ctcttataaa aaacacacag aagaagtaag ataattataa acgtaaattt
      541 aatatctccc actgacatct cccccacaat acactaaaaa atactcacag cagtaaacac
      601 agacacacct gataatcata cagccgataa atattttccc ctttacggag aaatcagcct
      661 gctgtttaac aataacggcg tattttcatg gaaggagatc acccagtaac ggataagtcc
      721 tgatctcctt catgttaata cgcaacaatc ccatcgattc aaaacaattt cagacagata
      781 ttatctc
//
LOCUS       DAZKGS010000532          786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKGS010000532 DAZKGS010000000
VERSION     DAZKGS010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>786)
                     /locus_tag="ACHOQY_005468"
     CDS             complement(<1..>786)
                     /locus_tag="ACHOQY_005468"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9681318.1"
                     /translation="FKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARS
                     QNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVN
                     SENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLK
                     NIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINT
                     YDNTIRYTDYIISKLIDKLIEYKDEYDTVLLYVSDHGESLGENG"
BASE COUNT          243 a          153 c          120 g          270 t
ORIGIN      
        1 ccattttctc ccagagattc tccatggtca gagacatata ataaaacagt atcatactca
       61 tctttatatt cgataagctt gtcaattaat tttgatatta tataatcagt atagcgaatt
      121 gtattatcat aggtattaat caattgctca tgcgtacaat tttcgatatc actacgatca
      181 catgttggtt taaaatattt atgtggctca ggataacgga gatagtaggt aggaccatga
      241 ctacccatca aatgaaatac gataagttta tcctctgagt tattatttat atactcatca
      301 atatttttta gcataacctc atcataacat gagtttttct tgcataatgt attatctgaa
      361 tttgatggct caatattaat tgtagggatt cgcttacata cacctttaca tcccccgtca
      421 ttttcaatcc agagtaaatt tacccccgtt ctgtacagaa tatccagtac attttccgaa
      481 tttaccgctt tgcgggaatt gaactctttt cgcttcatat ctgaaaacat gcatggaaca
      541 gatatggctg tggatgtgcc acacgactgc acattatgaa aggatataag ttcattatat
      601 ttctttgtaa aatcattggt accacgagaa tatccattca gagcatagtt ttgacttctt
      661 gctgtctccc cgacaactaa aaacataatt accggctttt gtttttcatt tattgtgcgg
      721 gatgcatccg ttccagtctg acgaaactcg ccaggagaaa caaaaaaatc ccttacatat
      781 ttaaac
//
LOCUS       DAZKGS010000533          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKGS010000533 DAZKGS010000000
VERSION     DAZKGS010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..784
                     /locus_tag="ACHOQY_005469"
                     /pseudo
     CDS             1..784
                     /locus_tag="ACHOQY_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          192 a          188 c          245 g          159 t
ORIGIN      
        1 atcctgagca acggggcgaa atataacccc acgacgtttt caccggtgga tgcgcagacg
       61 gtggaacaac tgaagatgac ggctgaaatt gtctgttcgg tgttccgtgt cccggcctac
      121 aagattggcg ttggccagcc gccttccagt gacaacgtgg aggcgctgga gcagcagtat
      181 tattcccagt gtctgcagac gctgattgag tccattgagc tgttactgga tgaggcgctg
      241 gaaacagggg aaaacgagag cacggagttt gacgtcacca cgctgctgag aatggacagc
      301 gaacggcgca tgaaaacgct gggtgaatcg gtgaaaaata cgcttctcac gcccaatgag
      361 gcccgtaaac gtgagaacct gccgcccctt gccggcggtg atgcactgta tcttcagcag
      421 cagaactaca gtctggacgc gctgtcccgt cgtgatgccc gtgaggatcc gttcgcgtca
      481 gccgggaaaa cagtttcatc acagctgcct gacggcgcat ctgacggtaa taaggcaatc
      541 agtgaaacag agcatgatgc agtgaaagcg atgttcaggg ggatactgag aaaatgacgg
      601 aacgggaact gtccattatt cgtgcactgg gcgaagaatt ctccacggtg ctggcggatt
      661 tacagtgcac atttgagggg aagatggccg cgcaggcaca agcgtttgaa gagaaactgg
      721 cttccctgtc ggcggtatta cagaagcatg tgacggtgga tgaggtgcgt ccggttctgc
      781 aggc
//
LOCUS       DAZKGS010000534          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKGS010000534 DAZKGS010000000
VERSION     DAZKGS010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..574)
                     /locus_tag="ACHOQY_005470"
     CDS             complement(<1..574)
                     /locus_tag="ACHOQY_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9681319.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRY"
     gene            complement(674..739)
                     /locus_tag="ACHOQY_005471"
                     /pseudo
     CDS             complement(674..739)
                     /locus_tag="ACHOQY_005471"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            710..>784
                     /locus_tag="ACHOQY_005472"
     CDS             710..>784
                     /locus_tag="ACHOQY_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681320.1"
                     /translation="MKHLSGIGFRIPAAPASLTFRVADN"
BASE COUNT          167 a          223 c          215 g          179 t
ORIGIN      
        1 cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc ggcagaatca
       61 gcgactgcat ggcatcacgc ttttcctcaa tccggtcaaa cacgcccacc acctcgtaag
      121 ccccttcaat gactctgtcc accacatttc cccggtgcgg aacacgcact tcccccagag
      181 actgaccaca gacgcatccg ttctggcaga cgaacctgaa gtaacccggc agcatctggt
      241 agctggaggt accgtcatga gagttgagca gaataatttc agggacatgt tctccgttta
      301 tctctccggc ccgccgcaga cgcagcatgt gttttgtgta tccccggcgg cccgggtcgc
      361 gcacacgggt ctggcaggcg aagaatggct gaaagccttc ccgctgcagg ctttccagta
      421 cggtgatggt ggggatgtac gtatagttct ggcttcggga cgtgtgttta tcttccccga
      481 aaatccccgg cacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct
      541 ggttcgcata accaaaacga ctggctagtc gcataatttg ctccttatcg gtggttaaga
      601 tttactggtg taataaatga aaaagccacg tctcccggag aagacgcggc ctgatgggtg
      661 aaatgaatga cgtttattgt ctgagaagac cgtcactggc gagccgcgta ttaagcacct
      721 ttccggcatc gggttcagaa ttccggcagc gccagcgtcg ctgaccttcc gcgttgcgga
      781 taac
//
LOCUS       DAZKGS010000535          784 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKGS010000535 DAZKGS010000000
VERSION     DAZKGS010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..232
                     /locus_tag="ACHOQY_005473"
     CDS             <1..232
                     /locus_tag="ACHOQY_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9681321.1"
                     /translation="GVAQMLAPKPKTPEYRATDNGRQNTYFSSLDNMIAQGNPMPVPY
                     GEMLVGSRRISQDISTRDEGGDGKMVVIGRQA"
     gene            471..>784
                     /locus_tag="ACHOQY_005474"
     CDS             471..>784
                     /locus_tag="ACHOQY_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681322.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TIT"
BASE COUNT          216 a          179 c          241 g          148 t
ORIGIN      
        1 cggtgtggcc cagatgcttg ccccgaagcc aaaaacaccg gaatacaggg caacggataa
       61 cggcagacag aacacgtact tttcctcgct ggataacatg attgcccagg ggaacccgat
      121 gccggtgcct tacggtgaaa tgctggttgg ctcccggcga atctcccagg acatcagtac
      181 ccgtgatgaa ggcggtgacg ggaagatggt ggttatcggg cggcaggcat aaaagcgaaa
      241 aaatcccgca gtgctcacgg acaggaactg cgggagcgtt acgaagattg agtgtaagga
      301 attattctta tgtcacgaca aaaaacatta acgcagagaa attattagcg ccacagtcag
      361 tttgtgaaaa tgtgaagata ttcagaattt ttattcagtc atgatacagg catcctccgg
      421 gatgcctgtt gtttttgtgc gtaacagtta tcacagtaaa gggtgagaca atgggcaaag
      481 gtggcggcaa ggcgcacacg ccggtagagg caaaggacaa tcttaagtcc acgcagatga
      541 tgagcgtgat tgatgccatt ggtgaagggc cgattgaagg cccggtgaag gggctgcaga
      601 gtatcctggt gaacaaaacc ccgctgacgg acacggacgg caatcctgtg atacacggtg
      661 tgaccgcggt ctggcgcgcc ggggagcagg agcagacacc accggaaggc tttgagtcct
      721 ccggagctga aaccggactg ggcgtggaag tgacgaaggc aaagccggta acgcgcacca
      781 ttac
//
LOCUS       DAZKGS010000536          781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKGS010000536 DAZKGS010000000
VERSION     DAZKGS010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..453
                     /locus_tag="ACHOQY_005475"
     CDS             <1..453
                     /locus_tag="ACHOQY_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9681323.1"
                     /translation="GETQGLISAGCSTLDSVGNKAQPEHKDQIMVYALMHSISRSQNV
                     NHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYYKINLSKARIS
                     SIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ"
     gene            497..>781
                     /locus_tag="ACHOQY_005476"
     CDS             497..>781
                     /locus_tag="ACHOQY_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681324.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLV"
BASE COUNT          265 a          158 c          149 g          209 t
ORIGIN      
        1 ggagagacac aagggctaat ttcagcaggt tgctcaacgc ttgattctgt cggaaacaaa
       61 gcgcagccag aacataaaga tcagatcatg gtttatgcgt taatgcattc catctcaaga
      121 agtcaaaatg tcaatcatca tgaattaatt atcaccaaac cggttgataa atcctcacca
      181 ttgctggcaa aagcacttag tgacaatgaa aaaatggcaa tatgtgaatt tatactctac
      241 cgaaccagta aagcgggtat ttaccagcct tattataaaa taaatctgtc aaaagcccga
      301 atatcaagta ttgattttgt cacaccgcat gctgtcctgg aaaaagaact ggaaccacag
      361 gagagaattg cctttattta tgaagatatt tcctgggagc atacgttagc cggtacgaat
      421 gcaatgagta aatggcagga tcgtgtacag taacttactg taataacata ataaattact
      481 attgcggaga gataaaatgg ctggtttaag gaataacaac aacacacaga atgcacagtg
      541 ggctgattat gtgggtgaca ttcttcgggg ggctcagccg ataaaccagc tagtacctca
      601 gcacccttat cttaacgacg tcccactcat tgatgagtta aggcatcaga acactcatca
      661 tgttattctc ttgacacttg atgttgcaaa aaaaatactc agcccaataa catcatttga
      721 ttacattcat tttatcacta cacatccttc aggtataaaa gatacactcg cctggttagt
      781 t
//
LOCUS       DAZKGS010000537          779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKGS010000537 DAZKGS010000000
VERSION     DAZKGS010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..>779)
                     /locus_tag="ACHOQY_005477"
     CDS             complement(38..>779)
                     /locus_tag="ACHOQY_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP9681325.1"
                     /translation="VFLIEQINDLKMWVNKYIDDCTDEDLNDRDFLASVVDRAIFHFA
                     INSICNLGDNKDAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGF
                     LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSF
                     DPTSKEEIKILTDTSKLIYDLFGVKSEK"
BASE COUNT          249 a          162 c          116 g          252 t
ORIGIN      
        1 atataatgcc ttccatacta tcgaagaact tcacatatta tttctccgat ttaaccccga
       61 acaaatcata aattaattta gaagtgtctg taagtatttt aatctcttcc tttgaggttg
      121 ggtcaaacga cttcgcgaag ctaattaatt gtggtgcagc atctcgcatt ttgcttaaaa
      181 tatcaggatc gagcgttcct tcattcacca gatgtgacat cttatccaga tagccatcaa
      241 aattcatttc tcccccatca gccagacgct tcatctctcc caggtacttc ttcatatcac
      301 gttgagataa tttttcaaac tgagctttca ggtagctctc attattttca ttaatataca
      361 ctgtttctga taaatctgcg taggcactag aatatgaaag tgaaattaac agcgaagcaa
      421 ataattttaa gctgttgtca tcatgttcct ggcaggcatt aacaaacgtt aaaaaaccag
      481 aacctattgc ttgaaaatgt atattcgcta aaggttcatt atccttagat tcctcataaa
      541 atagctgaac cgtggaggga aggtcattct tattttctac atcaaaagta cattgttcaa
      601 tgggcattgc atctttatta tccccaagat tacatatact attaatcgcg aaatgaaaaa
      661 tagcccggtc aacaactgat gcaagaaagt cacgatcgtt taaatcctca tcagtgcaat
      721 cgtcaatata cttattgacc cacattttga gatcatttat ttgctcaatt aaaaacaca
//
LOCUS       DAZKGS010000538          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKGS010000538 DAZKGS010000000
VERSION     DAZKGS010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..165)
                     /locus_tag="ACHOQY_005478"
     CDS             complement(<1..165)
                     /locus_tag="ACHOQY_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681326.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLP"
     gene            complement(137..643)
                     /locus_tag="ACHOQY_005479"
     CDS             complement(137..643)
                     /locus_tag="ACHOQY_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9681327.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTGFCIF
                     ALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLD
                     EYIRATTE"
BASE COUNT          206 a          189 c          151 g          229 t
ORIGIN      
        1 tggcagcgtt ttccattcgc cctcaccata tgaagattct ttaggcagat aataattttg
       61 atcagcccat tcaactggcg tcaccggcaa tgcccttatc aggggctgta atgcagttgt
      121 gacagcgctc atcatattat tcagttgttg ctctgatata ttcatcaagt aaatccggta
      181 atttatcccc tgcccgcgca cactgatttg cccccttagc aataagggtt ttcagatggt
      241 caagatggcg cggtgttaaa tcaggaaact gtcgctgcat ggataaaggg atggaatcaa
      301 gcgtactgga taacgccatt gccagcttac tgagggcaaa aatacagaat ccggtgtcaa
      361 taagcttacc ttcggttacc tgatttttaa gtttttgagc tacggcctgt tcttctgtca
      421 gttcagctct ggcccgaagc agcctttcct ccagatcgcc ctcgtcatca ggtattctct
      481 gattgtgttg tcgccgctcg cgatctatct ccagtacagt tttaacgtca taaaaaactt
      541 ccctcccccg acgctcaaca ggaggaacgc cccatttatc aaatgcctga acagatatac
      601 cgatggagga ggccatatca cttttattca ataaaaaggc catctcctct ccataagcca
      661 tcgataaaaa gcgatacaac aaccatgtgt ttttacaaaa ccatttgata tcattgacat
      721 ttttcgcatt gacgacatca aaacacatcg taaggttgtt gtatttattt tattt
//
LOCUS       DAZKGS010000539          773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKGS010000539 DAZKGS010000000
VERSION     DAZKGS010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..316
                     /gene="escR"
                     /locus_tag="ACHOQY_005480"
     CDS             <1..316
                     /gene="escR"
                     /locus_tag="ACHOQY_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP9681328.1"
                     /translation="TEKENVEFFERAAQKKLGNETILKKDSLFILLPAFTMGQLEAAF
                     KIGFLLYLPFIAIDLIISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLV
                     VN"
     gene            316..585
                     /gene="escS"
                     /locus_tag="ACHOQY_005481"
     CDS             316..585
                     /gene="escS"
                     /locus_tag="ACHOQY_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP9681329.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            585..>773
                     /locus_tag="ACHOQY_005482"
     CDS             585..>773
                     /locus_tag="ACHOQY_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscT/YscT/HrcT family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9681330.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPS"
BASE COUNT          221 a          132 c          145 g          275 t
ORIGIN      
        1 tacagagaaa gaaaatgttg agttttttga acgtgcagct caaaaaaaat tgggtaatga
       61 aactatctta aaaaaagact ctctatttat actgttacca gctttcacga tggggcagct
      121 tgaagctgca ttcaagatag gttttttgct ctatttacct tttattgcga tagatttgat
      181 catttccaat atcttattgg ccttgggtat gatgatggta tcgccagtaa caatttcgat
      241 tccttttaag atcttgttat tcattcttgt tggaggctgg caaaaattat tcgaatttct
      301 gttggtggtg aattgatgga aactggatat tttgttcaat tatgtgtgca aacgctttgg
      361 ataatattta tcctctcact gcctacagtc atagcggcct ctgttgtcgg cattattatt
      421 agcctggttc aggctataac gcagttacag gatcaaacat tgcctttttt gctaaaaata
      481 atagcagtgt ttgctacgct tgccctgact tatcactgga tgggaacaac aatcatcaac
      541 ttctcgtcga taatatttga aatgattccg aaggtgaatg gctaatgaat gaaataatga
      601 cggtcatagt atcatcattt ttttgtatac tcaggccgct ggggatgttt attattctac
      661 caatattttc aacaggtgtt ttgctcagta actttatacg caactcaatt atgattgcgt
      721 ttacacttcc aattatagta gagaactata cattttcgga aaaattacca tcc
//
LOCUS       DAZKGS010000540          763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKGS010000540 DAZKGS010000000
VERSION     DAZKGS010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>763
                     /locus_tag="ACHOQY_005483"
     CDS             <1..>763
                     /locus_tag="ACHOQY_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9681331.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREF"
BASE COUNT          180 a          205 c          233 g          145 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaagccgaa gcatgaggtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgcc
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagcctttg atccggttga ggtggatatg ggccgcagtg cggcaaacaa catcacacag
      301 tccggtggca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggcgtg gtgaatatca tcgtgtttga tccgaaagga
      421 tgggcgctgt tccgttcctt caaagccgtc agggataagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtctta taagggaatg
      541 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
      601 aacttcctgc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgtacc
      661 tatggctgta ttcaggatgc ggacgcacag cgcgaaggca ttaacgcctc tgcccgttac
      721 ccgaaaaact gggtgactac cggcgatccg gcgcgagagt tca
//
LOCUS       DAZKGS010000541          763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKGS010000541 DAZKGS010000000
VERSION     DAZKGS010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..582
                     /locus_tag="ACHOQY_005484"
     CDS             124..582
                     /locus_tag="ACHOQY_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681332.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            682..763
                     /locus_tag="ACHOQY_005485"
                     /pseudo
     CDS             682..763
                     /locus_tag="ACHOQY_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          252 a          130 c          129 g          252 t
ORIGIN      
        1 tgataatgat tatctctatc attatcgtga aattcgagaa tgaaaggaga agtagtcatt
       61 ataagccttt tataaatcgt catcatcata gtaaagtaga acacttttta tatgagttac
      121 tatatgaaaa tatactggac aagaaaacgt attcctgaac tttcagcact tccaccttca
      181 ttaagaaaga aaaattttac tgacgcatat aatgcagcat cttctcatat cgaatactgg
      241 ataggtgctg gcgtctcatt tatctcgatg atgattttat ccagagtata tgactttctt
      301 ttacccgccc aagatacttt cccgggtgat atcatcagaa gtttatgtgt tgtgtgtcca
      361 tccatattaa tctggtttca gttttcggta tatgtcatga ggaaatatta cagacatatt
      421 ctggtgcgag gaaaagaaac cgaaaccatt tcagaaaggt tgatacgtga ggctgataca
      481 cgagaatatg aactatggcg tccggtccgt cgctttttct ccattgtttt cctcattgtt
      541 ctattaggtt gcattcattc actgataaca actataaaat aaaagttatg gctgttctga
      601 tacaggaaaa taaacgttca ttctatggtt acctcttcct gttatataga agaattttaa
      661 acgacaagta agggaatata aatgaaaata acaaaaaata aaaatgcaac tataaaaaac
      721 agtcatcagt cggctgcttt tcaatgttta ccttatcatt ttc
//
LOCUS       DAZKGS010000542          757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKGS010000542 DAZKGS010000000
VERSION     DAZKGS010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..526)
                     /locus_tag="ACHOQY_005486"
                     /pseudo
     CDS             complement(1..526)
                     /locus_tag="ACHOQY_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(535..>757)
                     /locus_tag="ACHOQY_005487"
     CDS             complement(535..>757)
                     /locus_tag="ACHOQY_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9681333.1"
                     /translation="VYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFLI
                     LIIYFAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          261 a          144 c          128 g          224 t
ORIGIN      
        1 ctccccaatc tgataataaa gatatgatat ctccatcaag agttttaaaa agcagtgatt
       61 tatgattact ccaaaatact ttgatcgcca atgcaaaaaa aacaatataa agaattgctt
      121 taacaaattc ttttacggtt tttaagccaa atatacgctt taagccattt actggattca
      181 atgaatcaaa cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg
      241 agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg aatgttcgtt ttcaaccaac
      301 caacccccat agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaggatgt
      361 attcaagaat cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca
      421 caagcattat tacagagatg gttaagtctc tactttatag aaattgccct tttttagaag
      481 catcctggag tttcttttgt gttggctttt cagttttatt ggccatgaaa cactctattt
      541 ctcaatgtat agttgcaatt gttctgggaa aaaggataac ggcattactc gttcagccag
      601 tattggggca aaataaataa ttaatattaa aaatgccagc gcacttttga cagtcagtga
      661 aatggcaaaa gcatttagtt gtgaagcata acgtgccagc aatcccagga cagcctcacc
      721 gcccagcata acggcaatca cggggcttgc gtaaacg
//
LOCUS       DAZKGS010000543          756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKGS010000543 DAZKGS010000000
VERSION     DAZKGS010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..495
                     /locus_tag="ACHOQY_005488"
     CDS             61..495
                     /locus_tag="ACHOQY_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFP9681334.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          168 a          168 c          236 g          184 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacagctc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagt
//
LOCUS       DAZKGS010000544          754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKGS010000544 DAZKGS010000000
VERSION     DAZKGS010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..125)
                     /locus_tag="ACHOQY_005489"
     CDS             complement(<1..125)
                     /locus_tag="ACHOQY_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9681335.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYT"
     gene            385..720
                     /locus_tag="ACHOQY_005490"
     CDS             385..720
                     /locus_tag="ACHOQY_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9681336.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          286 a          154 c          110 g          204 t
ORIGIN      
        1 gtataagggg ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt
       61 gtcagctttt ccgcaagatc tgacggcgac gcagccctga caacatcata gtgtttaaat
      121 gccatggttc tttccaccat ctgaaaaata attctttaaa atacctgaca tgtaatacag
      181 aaaaaacaca aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata
      241 aactacaaga cgagatataa accaccctgt atttaaataa acaataaaca acatcagaaa
      301 aataattctg ctctatggtt taattcaaaa atatcattta tacttttcag aacatcacca
      361 gcaaggcata aacaaggaaa gtaaatgaag tggattgtga ttgatacagt tatccagcca
      421 tcatgcggaa tatctttttc agtcatatgg agtaaaataa aattaataat ctggtatcaa
      481 tcggatgctt tcttacctcc tgaaagtata tttacactga ctcacacagg catcatgctc
      541 aataacaaag tgctacctgt aaccatttac aacgtagtac cattcaataa aacattctgg
      601 aatttaatca aaaacagcca ggaatgccct acaaatacag ataacgtatt gaatgaatgc
      661 tttaataacc gttgcactct gcaaatatgt ccttatggac taaaacaaca aagtccataa
      721 ggagtttact cacatctgac aaaatcaata taaa
//
LOCUS       DAZKGS010000545          751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZKGS010000545 DAZKGS010000000
VERSION     DAZKGS010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     repeat_region   78..655
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=82..106
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          168 a          214 c          200 g          169 t
ORIGIN      
        1 tgtttgtgtg atactataaa gttggtagat tgtgactggc ttaaaaaatc attaattaat
       61 aataggttat gtttagagtg ttccccgcgc cagcggggat aaaccgatct cctcatcctc
      121 ccagctaccg acagtagtgt gttccccgcg ccagcgggga taaaccgcaa gatctgccgc
      181 gagtggtttc acccggcagg tgttccccgc gccagcgggg ataaaccgga tatttgggcg
      241 taataccgcc cggatagtgt gtgttccccg cgccagcggg gataaaccgt ggtacggaac
      301 atgctgtcta tatttcgacc agtgttcccc gcgccagcgg ggataaaccg ccagtggagc
      361 cgccgcgaat ttacacacga ttgtgttccc cgcgccagcg gggataaacc gtaaggccgt
      421 cgccggatca gcctggctat gccgagctcc ccgcgccagc ggggataaac cgttcttgcg
      481 ggtgttgcaa atattcttca cgtagagctc cccgcgccag cggggataaa ccggagcctg
      541 acgagactac tgaggccgtt ctgtcgagtt ccccgcgcca gcggggataa accggacgcc
      601 gccgccgcga agccgtttcc gatgttgagc tccccgcgcc agcggggata aaccaccata
      661 taacccgtta tctctttctc aagtttttat attagcagta cttgtaataa gcaacatatc
      721 cacgtaacac ctcatgttca aaatagttct c
//
LOCUS       DAZKGS010000546          749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKGS010000546 DAZKGS010000000
VERSION     DAZKGS010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(350..>749)
                     /locus_tag="ACHOQY_005491"
     CDS             complement(350..>749)
                     /locus_tag="ACHOQY_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFP9681337.1"
                     /translation="WNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGGFC
                     KYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFT
                     CTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          273 a          170 c           95 g          211 t
ORIGIN      
        1 attcaaaaaa aatactgaca acaaagatac tcttcagaca aagttgtcat atgtaaattt
       61 tcccttaaaa cgttgctcaa aaaaaatata aaaatcatat gccctaatca acatcctcaa
      121 gcactacgat atatgcagta tcctattcat atattaccac catatcagac aaatcgaatc
      181 acaacgagtt ctatcgtact ctttcatctt cgacactgta aattttaaga acattcggct
      241 catgccacta aaccaagaca taataacaac ggagcaataa atgttcccac tgaacatgtg
      301 caaacctaaa aagacagtgt tacaataata ttcatccatg gtctctaaat caacgccacg
      361 caacaggata atacaatgaa aatatttcag gaaaagattc ttttgcatgc tccgttagca
      421 tatcagtcag cacagcggta caagtaaaaa cattatttct tccttgtagc ctgtctaaaa
      481 tatcattgta ataataaact gagccaaata cctgaggatc tgttttataa gcttgttcaa
      541 tcaatccatt tgcatatctc cgtagaatct caggagaatc atactctgta ccaaacatat
      601 cactcgatga gtatttacag aaaccccccc ctaaacaaaa taatatttca gcctgttttc
      661 ttagtggcat atctttcaat acatggctcc ccataatcat atctacatgc tgagcgttca
      721 gttcccaacc atcaagaaag ggattccag
//
LOCUS       DAZKGS010000547          748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZKGS010000547 DAZKGS010000000
VERSION     DAZKGS010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            73..>748
                     /locus_tag="ACHOQY_005492"
     CDS             73..>748
                     /locus_tag="ACHOQY_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9681338.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDD"
BASE COUNT          249 a          127 c          164 g          208 t
ORIGIN      
        1 tataatatgc cgaattaaca catgttaacg atttaccagt aatgtaaata aattttcgag
       61 gagatcattc cagtgggacg taaatgggcc aatattgttg ctaaaaaaac ggctaaagac
      121 ggtgcaacgt ctaaaattta tgcaaaattc ggtgtagaaa tctatgctgc tgccaaacaa
      181 ggtgaacccg atccagaatt aaacacatct ttaaaattcg ttattgaacg tgcaaagcag
      241 gcacaagttc caaagcacgt tattgataaa gcaattgata aagccaaagg cggcggagat
      301 gaaacgttcg tacagggacg ttatgaaggc tttggtccta atggctcaat gattattgct
      361 gagacattga cttcaaatgt taaccgtacg attgctaacg ttcgcacaat tttcaataaa
      421 aaaggcggca atatcggagc ggcaggttct gtcagctata tgtttgacaa tacgggtgtg
      481 attgtattta aaggaacaga ccctgaccat atttttgaaa ttttgcttga agctgaagtt
      541 gatgttcgtg atgtaactga agaagaaggt aatattgtta tttatactga acctactgac
      601 cttcataaag gaatcgcggc gctaaaagca gctggaatca ctgagttctc aacaacagaa
      661 ttagaaatga ttgctcaatc tgaagttgag ctttccccag aagatttaga aatctttgaa
      721 gggcttgttg atgcccttga agatgacg
//
LOCUS       DAZKGS010000548          746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZKGS010000548 DAZKGS010000000
VERSION     DAZKGS010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>746
                     /locus_tag="ACHOQY_005493"
     CDS             <1..>746
                     /locus_tag="ACHOQY_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP9681339.1"
                     /translation="TAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGAS
                     TAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGQ
                     LTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTKTLSG
                     VLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFR
                     GTQIATARARAAVYRAQQAVAAARGTEMQIA"
BASE COUNT          149 a          174 c          265 g          158 t
ORIGIN      
        1 accgcttttt cggataatgc ggccctgttt gcccgttcgg ctgcctcgat gcgtgaatat
       61 ggttacagtg ctgatgatgt gctgaaggtg acggaggcca tttccacggg gctgaaaatc
      121 tccggtgcca gtacggctga ggcgggttcg gtgatcaccc agttcagcca ggcgctggca
      181 cagggtgtgt tgcgcggtga ggaatttaat tcggtcaatg aaagtggtga ccggatcatt
      241 cgtgcactgg ctgcaggcat gggcgtggcc cgtaaagatc tgaaggcgat ggcggacgat
      301 ggtcaactga cggcggataa agtcgttcct gcgttaatca gccagctggg ggtattgcgt
      361 gatgaatatg ccgccatgcc agaaacggtt tccggtagta tcacgaaggt ggaaaacgcc
      421 tttatggcct gggtgggcgg agtgaatgag gccagcgggg tgacaaaaac gctctccggc
      481 gtgctgaacg gtgttgccgg acagattgat aatgtggcaa cagctgtggg tgcgctggtt
      541 gccgtcgggg ttgcccggta ctttggcaat atggcctccg gagcgatgtc tgccacggca
      601 ggacttgtga cggctgcacg taatgaagtt gcactggcgg aagcacagtt caggggaacg
      661 cagattgcca cagcgcgggc aagggcagcc gtgtaccgtg ctcagcaggc cgtggcggca
      721 gcccgcggga cggagatgca gattgc
//
LOCUS       DAZKGS010000549          743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZKGS010000549 DAZKGS010000000
VERSION     DAZKGS010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>743
                     /locus_tag="ACHOQY_005494"
     CDS             <1..>743
                     /locus_tag="ACHOQY_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681340.1"
                     /translation="EQVLSKEQRKVIFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFND
                     IVPMILALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSG
                     YTPANAHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKI
                     ISDLDKVQVACYYLTILLAFERRTSLTSILATLYKMLISEREVSEYECQLLYLTNPID
                     VMNILNKYIYYFPNENSPFYTLKIDSALS"
BASE COUNT          283 a          128 c          112 g          220 t
ORIGIN      
        1 agaacaagta ttgagtaaag aacaacgaaa agttatattt aggagatatg aagatctcta
       61 tgttaagcta atgcactatt ctgtttttac agacaaaaca catcaaataa taaaacaaaa
      121 atattttaat gacattgtac caatgattct cgcactcgac atcaggaaca catatcgccc
      181 ggataatgag atggcatttt actatcatat tcattctttt ctcactcaga taccggataa
      241 tgaggatgat atatatcatg ctgcaaggac atatctgcga aattacgtta agttatgttt
      301 atccggatac acgccagcga atgcgcattt caaagatatc tttgatggcg tatatgaatt
      361 cattcgtaat attcgcaaaa acagtacacc aggaaaaaca aaacttatcg caactatcaa
      421 cacatgcaaa gaaacctgta aacatctgct ttatttaagt aatgaagaca aggaaaaaat
      481 aatttctgac ttagataaag ttcaggttgc atgttattat ctcactatat tactggcttt
      541 cgaaagacga acttcattaa caagcatcct ggcaacttta tataaaatgc tgataagcga
      601 aagagaagtt tcagaatatg aatgccagtt attatattta accaacccaa tagatgtaat
      661 gaatatactg aacaaataca tatattactt tcctaatgag aactcaccat tttatacact
      721 gaaaattgac agtgcattat cgt
//
LOCUS       DAZKGS010000550          741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZKGS010000550 DAZKGS010000000
VERSION     DAZKGS010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>741
                     /gene="rfaL"
                     /locus_tag="ACHOQY_005495"
     CDS             <1..>741
                     /gene="rfaL"
                     /locus_tag="ACHOQY_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP9681341.1"
                     /translation="MKEIFKAIIENTLLCTIAIPIILRDEKREDVEKIVFFSFISALG
                     LRCFSELITYYKDYQQGIMPFADYRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVL
                     SVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMT
                     AKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWT
                     FRQSIGPHNFALFIWFGTGLLGLVSLMML"
BASE COUNT          220 a          122 c          144 g          255 t
ORIGIN      
        1 atgaaggaaa ttttcaaagc tattattgag aatactttac tatgcacaat agcaataccg
       61 atcatattga gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt ctcatttatt
      121 agtgcgttgg gcttacgctg tttttctgaa ttgattacct attataagga ctatcaacaa
      181 gggataatgc cattcgcaga ttatagacac cgtagcattt ctgactcgat ggtcttttta
      241 ttccctgcat tgttaaatct ctggcttatc aaatcagcaa aataccgcat ttcttttgtg
      301 gttctaagcg ttatttttat ttttctgata ttaggaactt tatccagagg ggcctggctt
      361 tccgtgttag tcattggatt aatatggatt ctgatgttta aacaatggaa gttactatta
      421 gtaggagtaa tggttgccat cattgcattg tcggttattt tcacacataa ggagatgacc
      481 gcaaagctaa cgtataaact tcaacaaact aatagttctt atcgctatgc aaatggtact
      541 caaggcagcg cactcgatct aatattagaa aatcctgtta ttggttatgg ttacggtaac
      601 gttgcatata aagatgtcta taataaacgt gtcattgatt atccagaatg gacctttaga
      661 caatcaatag ggccacataa ttttgcgcta ttcatctggt ttggcactgg tttattaggg
      721 ctggtaagtc ttatgatgct a
//
LOCUS       DAZKGS010000551          739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZKGS010000551 DAZKGS010000000
VERSION     DAZKGS010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..637
                     /locus_tag="ACHOQY_005496"
     CDS             <1..637
                     /locus_tag="ACHOQY_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9681342.1"
                     /translation="LYATQNSPAQLLKIARSGDIIGRAPLPFISDAETIEHIQGNIFA
                     AVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKERRP
                     TGMFAYQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKFNLT
                     EMSFVEMMDLTKGNHSLTSDLLQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          243 a          153 c          142 g          201 t
ORIGIN      
        1 cctgtatgcc acccagaata gccctgcgca gctacttaaa atagccagaa gtggcgacat
       61 catcggccga gccccgctgc cttttatctc tgatgccgaa accattgagc acatacaagg
      121 gaacattttt gctgcagtag acgaaaagac ttctgaacta ttctttttta cagttacaaa
      181 agatatgcat atctccttgc gaaataaaat tcagttagaa aaattcaata aaaaaaatag
      241 agggtttgaa ggactggcat ggaaagcaga aggccggatg ctgtttgtcg ctaaagaacg
      301 acgccctaca ggcatgtttg cctatcagct ttctccagat ctgctgagtg caaaacaagt
      361 aacgattcca gaggaactaa atgatataca tgttaaagat atatccggcc tggactttaa
      421 taatgaaagt ctgatgattc tgtccgatga atcgcgtaag ttactcaagt ttaacctgac
      481 agaaatgtct ttcgttgaga tgatggacct gacaaaagga aatcattctc tgaccagtga
      541 tttgctccaa cctgaaggca ttgtaactct gtctgatgaa agcatttatg tcgctagtga
      601 acctgatatc ctcgctaaat ttgtccctaa taaataagtc tgtcactagc cttagtattg
      661 agcgaccaaa tctggcaatc tcatgaaact cgcaaaatac gaatctaata ttaataaaaa
      721 ttttactctg aaataaaca
//
LOCUS       DAZKGS010000552          738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKGS010000552 DAZKGS010000000
VERSION     DAZKGS010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            340..555
                     /gene="essD"
                     /locus_tag="ACHOQY_005497"
     CDS             340..555
                     /gene="essD"
                     /locus_tag="ACHOQY_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP9681343.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            555..>738
                     /gene="rrrD"
                     /locus_tag="ACHOQY_005498"
     CDS             555..>738
                     /gene="rrrD"
                     /locus_tag="ACHOQY_005498"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP9681344.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          186 a          142 c          178 g          232 t
ORIGIN      
        1 atttaaatcg gctaaaaata acgctggaaa ttatttgttg gttatttgtt gagatttgtt
       61 tatgtatttg tagtggtgtt ttcaatactc ggtagcattc tcgcaaatat catttagtgg
      121 tttacgtacg taaaaaattg gttatgctgt taagagtggt tacttcgtca cacagcttaa
      181 acccgccgtc gagcgggttt ttccattttt tgagtctcga tattagctga taacccaata
      241 cctgagttat tcactgactc cgagtctgtt acgtttctgc ttttttgcga tacgttgtat
      301 tccctcaatt tacacccgct ttgtctgcga ggtggggtta tgaaatccat ggataagtta
      361 acaacgggtg tcgcctatgg cacctcagca ggtagtgccg gttactggtt tttacagctg
      421 ctcgataaag tcacgccctc acagtgggca gcaataggtg tgctgggtag cctggtattt
      481 ggcctgctga cgtacctgac aaacctttat ttcaagatta aagaagataa gcgcaaggct
      541 gcgagaggtg aataatgcct ccatcattac gaaaagccgt tgctgctgct attggtggcg
      601 gagcaattgc tatagcatca gtgttaatca ctggcccaag tggtaacgat ggtctggaag
      661 gtgtcagcta cataccatac aaagatattg ttggtgtatg gactgtatgt cacgggcata
      721 caggaaaaga catcatgc
//
LOCUS       DAZKGS010000553          738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKGS010000553 DAZKGS010000000
VERSION     DAZKGS010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(74..>738)
                     /gene="queE"
                     /locus_tag="ACHOQY_005499"
     CDS             complement(74..>738)
                     /gene="queE"
                     /locus_tag="ACHOQY_005499"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9681345.1"
                     /translation="PINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTWEKL
                     EDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLLPLT
                     DLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKHPVG
                     RVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLN
                     IA"
BASE COUNT          180 a          200 c          169 g          189 t
ORIGIN      
        1 gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct
       61 tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat
      121 tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct
      181 gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc cagtagttca tccagtgctt
      241 caatatcacg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg
      301 acaacacgtc atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg
      361 gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa ccgttctttt
      421 cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca
      481 catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca ctgctcgccg
      541 ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat
      601 cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct
      661 gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga
      721 acatctcgtt aatcgggt
//
LOCUS       DAZKGS010000554          738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKGS010000554 DAZKGS010000000
VERSION     DAZKGS010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>738
                     /locus_tag="ACHOQY_005500"
     CDS             <1..>738
                     /locus_tag="ACHOQY_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFP9681346.1"
                     /translation="IIHNIENSIISGHESSDIIRSHQHEWGDLSTVEQFKKFEFYVKS
                     ELSFSKSIFDDIKTKYITDPETKRNALYHQLDSDIKERIAFLDISHYAYPGSLLEKLQ
                     LSGYVFSDINIIAEYLLSSYGISGHYSHGVVYPAPSDKLFELLRRHTNSNSDWIEKII
                     PYVYDILSGNVSSFLHPPLSEEQKKILSDIKLEISESVSEQYFMKLTEQKSSVIGIKY
                     SVDFDRYNENLFLSLPINQNLTLPFMY"
BASE COUNT          269 a          121 c          109 g          239 t
ORIGIN      
        1 gcattataca caacatcgaa aattctatta tatcaggtca tgaatcatct gatataatac
       61 gaagccatca acatgagtgg ggagatttat caacggtaga gcaatttaaa aaatttgaat
      121 tttatgttaa aagtgagtta tcattttcca agtcaatttt tgatgatata aaaaccaaat
      181 atataacaga tcccgaaaca aaacgaaacg cactttacca tcaattagat agtgatatca
      241 aagagcgaat agcctttctc gacatatccc actacgcata tccaggtagt ttattagaaa
      301 aattacagtt aagcggctac gttttttctg acatcaacat aattgcagaa tacttattgt
      361 ctagttacgg aatttctggg cattattctc acggcgtggt atacccagct ccatctgata
      421 aattatttga gttattaaga agacatacaa attccaattc tgattggata gaaaaaataa
      481 tcccatatgt ttatgatatc ttatcaggta atgtgtccag cttcttgcac cctcccttat
      541 cagaagaaca gaaaaaaata ttgagtgata taaaattaga aattagtgaa tctgtatcag
      601 aacagtattt catgaagttg acagaacaaa aatcatcagt tattggcatt aaatactctg
      661 ttgattttga tagatacaat gagaatttat ttttgtcttt acctataaat caaaatttaa
      721 ctctaccttt catgtatc
//
LOCUS       DAZKGS010000555          737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKGS010000555 DAZKGS010000000
VERSION     DAZKGS010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..441
                     /locus_tag="ACHOQY_005501"
     CDS             <1..441
                     /locus_tag="ACHOQY_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFP9681347.1"
                     /translation="ALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFKRHIRHRE
                     ADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYDLRYGHPI
                     NADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            419..>737
                     /locus_tag="ACHOQY_005502"
     CDS             419..>737
                     /locus_tag="ACHOQY_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681348.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAK"
BASE COUNT          215 a          150 c          203 g          169 t
ORIGIN      
        1 gcgcttcgtg aggcagcaca aaactatcag tcgacgctgg cgtggtatca ggctaccccg
       61 gacagcccaa atgctgaacg ggattgtgat gcggctcttg ctgcgtttaa gcgtcatatc
      121 cgtcatcggg aagcggatat tatcgctggt ttgctggatg gactggaaga agcaaaatca
      181 caactcaacg agcagcgtga gtattacgaa ggcgttatct ctgatgggag caagcgtatt
      241 gctgaactgg atgcgcggga agttcaatta ccgactcgct acgaccttcg atatggacac
      301 ccgataaatg cagatgagcg acatgtcatg atacctaaag aaaatggcag ttggctttac
      361 ctgattgacc tagaacacgc attacgcgtc gctggcattc gcatcaaagg agaggagcat
      421 ggaaataaaa ccagaggatg agttaagcaa tatcgtttta tttccggtaa aagaggatga
      481 ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt gtcatcacgc
      541 ctctgtccgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg gtgcagttgt
      601 ggagccattt gactggatgc tctctgtggc gaaaagagaa actaggctgg cagatgatgt
      661 aaagctattg cgccaggagg aacaggaaag gcggaaaaat atagaaaagt taattcagat
      721 tgagcgtaac gcgaaag
//
LOCUS       DAZKGS010000556          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKGS010000556 DAZKGS010000000
VERSION     DAZKGS010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>736
                     /locus_tag="ACHOQY_005503"
     CDS             <1..>736
                     /locus_tag="ACHOQY_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9681349.1"
                     /translation="AEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLSDSDPSKS
                     DASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQPNTQTAEQ
                     DFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVRPKEPNWT
                     AWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELSLRLSPSV
                     STYTEKREDPYFKSSYDNVDYSQIPAG"
BASE COUNT          199 a          196 c          190 g          151 t
ORIGIN      
        1 gcagaaggct ggttaacgcg taaggcgcgt cgccagggta accgcaatgc gtcgaatgtt
       61 tatcagctta acgttgcgaa gcttcaggca gcggcatttt ctcaactgtc agattctgac
      121 ccgtcaaaat ctgacgcatc aaaatctgac ccgtcaaaat ttgatgcgtc gaaatctggc
      181 aaaaaagcgg gttttcaccc gtcagaatct ggcggggatc cgtcagtaaa atcaaaacat
      241 gatccgtcag ataaaaaacc ttctcgtccg gacgcttcgc aaccgaacac gcagacggct
      301 gaacaggatt ttttaactcg ccatcctgat gcggttgtat tcagccctaa aaagcgccag
      361 tggggaacgc aggatgattt gacctgcgca cagtggctct ggaaaaaaat catcgccctg
      421 tacgagcaag ccgccgaatg tgacggcgag gtggttcgtc ccaaagaacc gaactggaca
      481 gcctgggcaa acgaaattcg cctgatgtgt gtgcaggatg gtcgtactca caaacaaatc
      541 tgcgagatgt acagccgcgt cagccgcgat ccgttctggt gccgtaacgt gctcagcccg
      601 tcgaagttgc gggaaaaatg ggatgagctt tccctgcgct tatcaccgtc cgtcagcacg
      661 tacaccgaaa aacgcgaaga cccgtacttc aaatccagtt acgacaacgt ggactacagc
      721 cagatcccgg caggat
//
LOCUS       DAZKGS010000557          732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKGS010000557 DAZKGS010000000
VERSION     DAZKGS010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..732
                     /locus_tag="ACHOQY_005504"
                     /pseudo
     CDS             3..732
                     /locus_tag="ACHOQY_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          193 a          202 c          198 g          139 t
ORIGIN      
        1 acatgacaaa aacagtatca accagtaaaa aaccccgtaa acagcattcg cctgaatttc
       61 gcagtgaagc cctgaagctt gctgaacgca tcggtgttac tgccgcagcc cgtgaactca
      121 gcctgtatga atcacagctc tacaactggc gcagtaaaca gcaaaatcag cagacgtctt
      181 ctgaacgtga actggagatg tctaccgaga ttgcacgtct caaacgccag ctggcagaac
      241 gggatgaaga gctggctatc ctccaaaagg ccgcgacata cttcgcgaag cgcctgaaat
      301 gaagtatgtc tttattgaaa aacatcaggc tgagttcagc atcaaagcaa tgtgccgcgt
      361 gctccgggtg gcccgcagcg gctggtatac gtggtgtcag cggcggacaa ggataagcac
      421 gcgtcagcag ttccgccaac actgcgacag cgttgtcctc gcggctttta cccggtcaaa
      481 acagcgttac ggtgccccac gcctgacgga tgaactgcgt gctcagggtt acccctttaa
      541 cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg agggcaaagg cctcccggaa
      601 gttcagcccg gtcagctacc gcgcacacgg cctgcctgtg tcagaaaatc tgttggagca
      661 ggatttttac gccagtggcc cgaaccagaa gtgggcagga gacatcacgt acttacgtac
      721 agatgaaggc tg
//
LOCUS       DAZKGS010000558          710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKGS010000558 DAZKGS010000000
VERSION     DAZKGS010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>710
                     /locus_tag="ACHOQY_005505"
     CDS             <1..>710
                     /locus_tag="ACHOQY_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681350.1"
                     /translation="IARTKAALQKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTAML
                     KQFRADLTVFNAQCHGGSAVNVPWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYF
                     VPFMTDGNGVNTATNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIP
                     DRMATAILNAAGRTSAFISGKAPEIKPSPGGDTPSGPSADTSVRTISLLPTAGEAAAQ
                     GWTIKDGGIQLSGGVFKI"
BASE COUNT          172 a          184 c          203 g          151 t
ORIGIN      
        1 tgattgcgcg cactaaagct gcattacaga agaacccgaa aaatgtgttg ctggcggtgt
       61 gctggatgca gggagagttt gacatgagcg ccgccaccta cgcacagcaa cctgcgctgt
      121 ttacagccat gctgaagcag tttcgtgctg acctcactgt gtttaacgcg cagtgtcatg
      181 gtggcagtgc tgtaaatgtg ccgtggattt gtggtgacac gacgtattac tggaaaaata
      241 catacgctac ccagtacgac accgtgtacg gcgggtataa aaacagggag agtgagggcg
      301 tttattttgt gcccttcatg acagacggta acggcgtcaa taccgccact aacgcaccgg
      361 cagaagatcc ggatattccg gcatcaggat attacggtgc ggcatcgaga acgaatggaa
      421 accaggtatc atcaaaccgc ccgacacatt tcagttcatg ggcgcgcagg agcattattc
      481 cggatcgtat ggcaaccgct attctgaacg cagccgggcg cacctcagct ttcatcagtg
      541 gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac gccatcgggt ccgtctgcag
      601 atacgtccgt tcgcacaatc tccctgctgc cgacagccgg agaggctgct gcgcagggct
      661 ggaccattaa ggacggcgga attcagttgt cgggtggtgt atttaagatc
//
LOCUS       DAZKGS010000559          707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKGS010000559 DAZKGS010000000
VERSION     DAZKGS010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..573)
                     /gene="fimE"
                     /locus_tag="ACHOQY_005506"
     CDS             complement(<1..573)
                     /gene="fimE"
                     /locus_tag="ACHOQY_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9681351.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINE"
BASE COUNT          213 a          175 c          143 g          176 t
ORIGIN      
        1 ttcgtttatg agattatttc tttcccataa tccggcaaaa cgagcagcat tactggcggt
       61 ataacgtaca gtatggcgaa tatttcgatg cccgagataa tcctgaatta aacgagtatc
      121 tgcaccacgc tccgccagtt cataaccgca agcatgcctt aacatatgag gatgagtctg
      181 cgttacggtt ccagcttcaa taccggcatc gcgaataatg cgataggcct gctggcgaga
      241 aagccgactc ccacggcgag aaataaatat agcgtcggtc cggtcagcgc ctttccagtt
      301 agcacgttcc tgggtccagc gttccacggc ttcacgctca tcaaaacgta acgggtgaac
      361 ggtagaaaat ccgttcttca gtcggcgaat atttattcga ccttcattaa ggtcaagatc
      421 ctgataatgc agatcaagca gttcactaat acgcatccca tgccgatatg ccaacagaat
      481 aagacaataa tctctggctc ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac
      541 ttctttaccg gtaagataac gacgtttact cacaatagta gtactcctga ctgagatata
      601 tttgaatgaa tacctatagg aaacctcaat cggtcaaaat tagcccaata gataaaaaga
      661 taaccacaca cgaagtgatg tggctatcag tcaattacat taaatat
//
LOCUS       DAZKGS010000560          701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKGS010000560 DAZKGS010000000
VERSION     DAZKGS010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..701
                     /locus_tag="ACHOQY_005507"
                     /pseudo
     CDS             1..701
                     /locus_tag="ACHOQY_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          177 a          168 c          218 g          138 t
ORIGIN      
        1 attctgagca acggggcaaa atacaacccc acgacgtttt caccggtgga tgcgcagacg
       61 gtggaacaac tgaagatgac cgctgaaatt gtctgttcgg tgttccgtgt cccggcctac
      121 aagattggcg ttggccagcc accttccagt gacaacgtgg aggcgctgga gcagcagtat
      181 tattcccagt gcctgcagac gctgattgag tccattgaac tgttactgga tgaggcgctg
      241 gaaacggggg aaaacgagag tacggagttt gacgtcacca cgctgctgag aatggacagc
      301 gaacggcgca tgaaaacgct gggtgagtcg gtgaaaaata cgcttctcac gcccaatgag
      361 gcccgtaaac gtgagaacct gccgcccctt gcgggaggtg atgcactgta tcttcagcag
      421 cagaactaca gtctggaggc gctgtcccgt cgtgatgccc gtgaagatcc gtttgcgtcg
      481 tccgggaaaa gagtttcagc ccaactgcct gacggcgcat ctgacggtaa taaggcaatc
      541 agtgaaacag agcatggtgc ggtgaaagcg atgttgaggg ggatactgcg aaaatgacgg
      601 aacgggaact gtccattatt cgtgcactgg gagaagaatt cgccacggtg ctggcggatt
      661 tacagcgcac atttgaggag aagatggccg cgcaggcaca a
//
LOCUS       DAZKGS010000561          701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKGS010000561 DAZKGS010000000
VERSION     DAZKGS010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>701
                     /locus_tag="ACHOQY_005508"
     CDS             <1..>701
                     /locus_tag="ACHOQY_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9681352.1"
                     /translation="YRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFE
                     HLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGP
                     WWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDG
                     AGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTH
                     DPEYPENLPAAPLVR"
BASE COUNT          127 a          215 c          240 g          119 t
ORIGIN      
        1 taccggacaa aaacgcccgc gccggtgggg agcctcggcc ccggctggaa aatgcctgcg
       61 gatatccgct tacagctgcg cgataacaca ctgatactca gtgataacgg cggcagaagc
      121 ctgtattttg agcacctgtt tcccggtgag gacggttaca gccgcagcga gtcactgtgg
      181 ctggtgcgcg gcggcgtggc gaaactggat gaaggtcacc ggctggccgc actctggcag
      241 gcgctgccgg aagaactccg cttaagtccg catcgttatc tggcgacaaa cagtccgcag
      301 gggccgtggt ggctgctcgg ctggtgtgag cgggtgccgg aagcggatga ggtgctgcct
      361 gcgccgctgc cgccgtaccg ggtactgacc gggctggtgg accgcttcgg gcgcacacag
      421 acgttccacc gcgaagccgc cggtgaattc agcggcgaaa tcaccggcgt gacggatggt
      481 gccgggcgtc acttccggct ggtactgacc acgcaggcgc agcgggcaga agaagcccgg
      541 cagcaggcca tttccggcgg gacggaaccg tccgcttttc ctgataccct gccgggttac
      601 accgaatatg gccgggacaa cggcatccgt ctgtctgccg tgtggctgac gcacgacccg
      661 gaatacccgg agaatttacc tgccgcgccg ctggtgcgct a
//
LOCUS       DAZKGS010000562          699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKGS010000562 DAZKGS010000000
VERSION     DAZKGS010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(312..>699)
                     /locus_tag="ACHOQY_005509"
     CDS             complement(312..>699)
                     /locus_tag="ACHOQY_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9681353.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZKGS010000563          698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKGS010000563 DAZKGS010000000
VERSION     DAZKGS010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..612
                     /locus_tag="ACHOQY_005510"
     CDS             <1..612
                     /locus_tag="ACHOQY_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFP9681354.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            633..>698
                     /locus_tag="ACHOQY_005511"
     CDS             633..>698
                     /locus_tag="ACHOQY_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681355.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKK"
BASE COUNT          234 a          140 c          164 g          160 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataatcaat gtaagaaa
//
LOCUS       DAZKGS010000564          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKGS010000564 DAZKGS010000000
VERSION     DAZKGS010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..>694)
                     /locus_tag="ACHOQY_005512"
     CDS             complement(4..>694)
                     /locus_tag="ACHOQY_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9681356.1"
                     /translation="VATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFH
                     NPVVLNSVTVGPQSTPYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSL
                     SVPLILFLAVFFGGLKPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSP
                     LVALIVAALLFGFYHYSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHL
                     LFFTYPFLKHN"
BASE COUNT          287 a          149 c          111 g          147 t
ORIGIN      
        1 aaatcaatta tgttttaaaa atggataggt aaagaataac aagtgacaca gattcaaacc
       61 aaaatggaaa agggtggcaa cccacaaacg cccactccac atccatgaca atccatacac
      121 aacaccagat aacgtggcaa ataatacaag taaagcacca cctgaatagt gataaaaacc
      181 aaacaacaaa gccgccacaa ttaatgcaac caatggagac gttacttctg atagccgtga
      241 ttgaatatac cctctaaata atgattcctc tgccagagac acaaaaaaca aattagccaa
      301 tataaactct ggcaaccact caggaaaatg aatctctggc tttaatccac caaaaaaaac
      361 agccaaaaac aggataagag gaacagagag cgacagagcc ccccacttcc acaaagacac
      421 ttctgatttt acttcttttt taaacaaaga agatgtacac aggactaaca aaaatggcac
      481 cagcgcttta tcaaaattaa aatacattgt atagggagta ctctgagggc caacagtaac
      541 agaatttagc actacaggat tgtgaaaccc tggccataga tggaaagata atgctatggc
      601 tgataaaact atgccaactt catatataga tttagcccag gcgttatatt tccagttgaa
      661 cttcaaaata ataaaaaaaa cgattgtagc aaca
//
LOCUS       DAZKGS010000565          687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZKGS010000565 DAZKGS010000000
VERSION     DAZKGS010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>687
                     /locus_tag="ACHOQY_005513"
     CDS             <1..>687
                     /locus_tag="ACHOQY_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9681357.1"
                     /translation="FQITAVPRLAVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGS
                     QWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGQASNDAEGYLDFFRGEIGKTHLA
                     QGLWELIDNSQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRVQKDTNDD
                     LAALYMLKVQKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLF
                     VAQGNQLFMN"
BASE COUNT          185 a          157 c          209 g          136 t
ORIGIN      
        1 ttttcagata acggcggtcc cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt
       61 ctggttttcg gaggcaaaaa tcgcagacac atctcaggtg gaaacctctg cccgttatct
      121 ggggaccggc agtcagtgga gtgtatccgg cccgcacatt aagcccggga aggatttctg
      181 gttttacgtg cgcagcgtca acctggtggg gaaatctgcg tttgtggaag ccagcgggca
      241 ggccagcaat gatgcagaag ggtatctgga ctttttcaga ggagaaatag gaaaaacgca
      301 tctggctcag gggttgtggg aactgataga taacagccag cttgcagatg agatggcgga
      361 gatgaagacc agcatcacgg aaacccgcaa tgaaatcaca cagacggtca acaaaacgct
      421 ggaggaccag agcgccacca tacagcagat acagcgcgtg cagaaggaca caaatgatga
      481 cctggctgcg ctgtacatgc tgaaggttca aaaaacgaaa gacggcattc cctatgtggc
      541 cgggattggt gcagggattg aggatactga tggccagcca ctgagcaaca tactgctgct
      601 ggctgaccgt atcgcgatga taaatccgga gaacggcaac agcacgccgt tatttgtggc
      661 gcaggggaat cagctgttca tgaacga
//
LOCUS       DAZKGS010000566          678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKGS010000566 DAZKGS010000000
VERSION     DAZKGS010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..311
                     /locus_tag="ACHOQY_005514"
     CDS             <1..311
                     /locus_tag="ACHOQY_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681358.1"
                     /translation="IRAGVQYYDRQTKKKEWTNYSAVVFAKPGAQADYYRSVLVEGGI
                     VEITGENIRVDVYQGQNGQSITLELLNAKIGFAASGNGPQQQSSNQQNTPVYDDSIPF
                     "
     gene            335..628
                     /locus_tag="ACHOQY_005515"
     CDS             335..628
                     /locus_tag="ACHOQY_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFP9681359.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
BASE COUNT          206 a          155 c          162 g          155 t
ORIGIN      
        1 acatccgtgc tggcgttcag tattacgatc gccagacaaa aaagaaagaa tggacaaact
       61 acagcgccgt tgtatttgcc aagccgggag cgcaagcgga ttactaccgt agtgttcttg
      121 ttgaaggtgg cattgtggaa attaccggag aaaacatcag ggttgatgtt tatcaggggc
      181 aaaatggtca atcaatcact cttgaattac tgaatgcaaa gattggattt gcagcttcag
      241 gaaatggccc gcagcagcaa agtagtaacc agcagaacac tcctgtatac gacgattcca
      301 tcccattctg atttagaaaa ataaggattt aattatgcca gcgcctctgt atggtgcgga
      361 tgacgcgcgc cgctgttccg gcaattccgt atcggaggtg ctggataaat tcagaaaaaa
      421 ctacgatcgg ataatgtcgc taccgcagga aacgaaagag gaaaaggaat ttcgccattg
      481 tatatggctt gcagagaaag aagaacgcga gcgaatttac cagacatcaa tccgaccatt
      541 ccgcaaagcc acatataccc acttccctga aattgacccg cgcctgcgta attaccgctc
      601 acgctatggc gctatcagta atgactgagg aatttaccat gagaggactt gcatacaatc
      661 ccggcattct tccggcag
//
LOCUS       DAZKGS010000567          676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZKGS010000567 DAZKGS010000000
VERSION     DAZKGS010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..419
                     /locus_tag="ACHOQY_005516"
     CDS             <1..419
                     /locus_tag="ACHOQY_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9681360.1"
                     /translation="KSHKIDAHIITFVAKMPYIDSIKLLEAGAKGCVWKTSHPAKLNR
                     AIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIADGKTNKEIANFLQL
                     SRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(438..676)
                     /locus_tag="ACHOQY_005517"
                     /pseudo
     CDS             complement(438..676)
                     /locus_tag="ACHOQY_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
BASE COUNT          223 a          116 c          121 g          216 t
ORIGIN      
        1 ttaaatcgca taagatagat gctcatatta ttacatttgt agcaaagatg ccgtatattg
       61 attcaataaa attgcttgaa gcaggtgcga aaggatgcgt atggaaaacc agtcacccgg
      121 cgaaactaaa tcgtgctatt gattcgatta gtaatggtta cacttatttt gatagtgtac
      181 atatggattg tgaaaaaata tcttccaggt attcctctga taatcaactt acaaatcgtg
      241 agtcagaaat attacaactc atagctgatg gtaaaacaaa caaagaaatc gctaatttct
      301 tgcagttaag cagaaaaaca gtcgaaactc atagactcaa tatcatgaag aaattagatg
      361 ttcacagtgg tatcgagttg atcaaaacag ctttacgtat gggtgtttgc actatataaa
      421 aaaaagaggc tttaggctca ttttgctgaa gccttacgat tgtctagttc attaagcctg
      481 gtatatacaa tgtatatttt ggggcatatt gctgtctctt tttttggtta tccattgcaa
      541 aacccgcatt ataagtcccc aggcattgcc acgttactcc acaacgtgaa aaatggttat
      601 ataaaatctc ggttcctatt ttaatgttca gacaagggtt atctaacaaa tctcgttcat
      661 cttttattat tccacg
//
LOCUS       DAZKGS010000568          671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZKGS010000568 DAZKGS010000000
VERSION     DAZKGS010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            243..668
                     /locus_tag="ACHOQY_005518"
     CDS             243..668
                     /locus_tag="ACHOQY_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681361.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDGKFQLKIEDLQGSDKLANFNFFLIKKSNLS
                     GLYMYHHGSCSLNTLFSHLETISNEFIRNQNKEEIKKTW"
BASE COUNT          244 a          122 c          107 g          198 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg taaattccag cttaaaattg
      481 aagatcttca aggaagtgac aaacttgcta atttcaactt tttcttaatt aaaaaatcta
      541 atctttctgg tctctatatg tatcaccacg gttcatgcag tctgaacact ctcttttctc
      601 atttagaaac aataagtaat gaatttatca gaaatcaaaa taaagaagaa ataaaaaaaa
      661 cttggtgata a
//
LOCUS       DAZKGS010000569          665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZKGS010000569 DAZKGS010000000
VERSION     DAZKGS010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..307
                     /locus_tag="ACHOQY_005519"
     CDS             <1..307
                     /locus_tag="ACHOQY_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9681362.1"
                     /translation="GSALAAGGFSATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTD
                     NGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          173 a          146 c          214 g          132 t
ORIGIN      
        1 gggttcagcc ctggcagccg gcggtttttc tgccaccacg atgctgtttt cactgggggc
       61 cagcatgata ctgggcggtg tggcccagat gctggccccg aaggcaaaga cgccggagta
      121 caaaagtacg gataacggta aacagaacac gtatttttcg tcactggaca acatgattgc
      181 ccaggggaac ccgatgccgg tgccttacgg tgaaatgctg gttggctccc ggcgaatctc
      241 ccaggacatc agcacccgtg atgaaggtgg tggcgggaag gtggtggtta tcgggcggca
      301 ggggtaaagc ataaaaaaat cccgcagtgt atggaggctg cgggaacaga aaatgaagat
      361 taaccacagg gggttttgtt tttattggcc cgaaaaaact gtaacgcccg ggaatgatat
      421 ctgccacggg gcgtacagaa aatgtgaaga aattcagaaa ttttattccg tcatgacaca
      481 ggcaccctcc ggggtgcctg tcgtttttgg ggcataaaca gattcagaca tcagacagga
      541 gagggggaca gagtgggtaa aggtggcggc agggcgcaca cgccggtaga ggcaaaggac
      601 aatcttaagt ccacgcagat gatgagcgtg attgacgcca ttggtgaagg gccgattgaa
      661 ggtcc
//
LOCUS       DAZKGS010000570          660 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZKGS010000570 DAZKGS010000000
VERSION     DAZKGS010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..155
                     /locus_tag="ACHOQY_005520"
     CDS             <1..155
                     /locus_tag="ACHOQY_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9681363.1"
                     /translation="IFIVLMIPIPPLLLVWFIVKIIEPVIELYNDVVWASFSTLHNKI
                     NPYKES"
     gene            157..522
                     /locus_tag="ACHOQY_005521"
     CDS             157..522
                     /locus_tag="ACHOQY_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP9681364.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
BASE COUNT          188 a          133 c          169 g          170 t
ORIGIN      
        1 ttatatttat cgtgttgatg ataccgatac ccccgctatt actggtctgg tttatcgtga
       61 aaataattga gccagttatt gaattgtata acgacgtggt gtgggcgtcg ttcagcacac
      121 tgcacaataa aattaatccg tataaggaaa gctgatatgg cactgacgaa aaaacaacgt
      181 gcagagctgc gcatgaagtt cggcggtcgc tgtgcttatt gcggctgcga acttggcgaa
      241 aagtggcatg cagaccatgt aaaaccggtc attcgttttg atggaaatat gcttcaccag
      301 gaacgtgacg atatatccaa catggttccg gcatgccacc catgcaatct gcacaagcat
      361 tgcagtagtc tggaagatta tcggcgaatt atcagtgatg gtcgtcgtga attccttgcg
      421 tccgggaaag gcaaagcgct ggttcgtatg ggattggttg aaatgaaatc tgacccagtt
      481 gtgttctggt ttgaaaaata tcaagaaggg gctacggcat gacgactttt accagagagc
      541 agttaatagc tcacgcagag gagactattg aaacacagag actgtgcata ccgggcacaa
      601 tcgaccatga catcatccgc acatataaga tggatattgc tgttctggaa atcgcactgg
//
LOCUS       DAZKGS010000571          653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZKGS010000571 DAZKGS010000000
VERSION     DAZKGS010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>653)
                     /locus_tag="ACHOQY_005522"
     CDS             complement(<1..>653)
                     /locus_tag="ACHOQY_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9681365.1"
                     /translation="RGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQI"
BASE COUNT          140 a          196 c          167 g          150 t
ORIGIN      
        1 taatctgccg gtcaatcagc cagctttcct cacccggccc ccatccccat acgcgcattt
       61 cgtagcggtc cagctgggag tcgataccgg cggtcaggta agccacacga tcaggaacgg
      121 gcgctgaata atactctttc cgctctgcca tcacttcagc atccggacgt tcgccgattt
      181 tcgcttccca cgtctcaccg agcgtggtgt tcacgaaggt tttacgtttt cccgtatccc
      241 ctttcgtctt catccagtct ttgacaatct gcacccaggt ggtgaacggg ctgtacgccg
      301 tccagatgtg aaaggtcaca ctgtcaggcg gttcaatctc ttcaccggat gacgaaaacc
      361 agagaatgcc atcacgggtc cagatcccgg tcttttcgca gatataacgg gcatcagtaa
      421 agtccagctc ctgctggcgg atgacgcagg cattatgctc gcagagataa aacacgctgg
      481 aggggtcatc cggcgtccat ttgaggccaa acggcgtctc tttgtcgcca aatttaagat
      541 actgctcctc cccgcagtgc gggcaggcaa catgaaaacg cataaaatgc ggggattcac
      601 tggctgcacg ctcaatctgg caggtgcctc tcactttggg cgtggagcca cgg
//
LOCUS       DAZKGS010000572          646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZKGS010000572 DAZKGS010000000
VERSION     DAZKGS010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            95..>646
                     /gene="dosC"
                     /locus_tag="ACHOQY_005523"
     CDS             95..>646
                     /gene="dosC"
                     /locus_tag="ACHOQY_005523"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP9681366.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKER"
BASE COUNT          180 a          129 c          158 g          179 t
ORIGIN      
        1 atatttcagg agaatatggt gcagttagtc gatctcgcac gttgcgtttc gttttgattc
       61 cgtctgattt cccagcctta taagggtgtg aattatggag atgtatttta aaagaatgaa
      121 agatgagtgg accggacttg tcgaacaggc agatcctctc attcgtgcta aagccgcaga
      181 aattgccgtt gcgcatgctc attatctgag tatcgagttt tatcgaattg tccgcatcga
      241 cccgcatgcc gaagaattct tgagtaatga acaagttgag cggcagttga aaagtgcgat
      301 ggaacgctgg attattaacg tgctttctgc ccaggttgac gatgtcgaaa ggctaataca
      361 aatccagcat accgtcgcgg aagtgcatgc ccgcatagga attccggtag aaattgtcga
      421 gatggggttt cgggtgctga aaaaaatcct ctatccggta atcttctctt cggattattc
      481 tgccgcagaa aaacttcagg tctaccattt ctcgattaac agtattgata tcgctatgga
      541 agtgatgacc cgcgcgttta cctttagtga cagtagtgcc tcaaaggaag atgaaaacta
      601 tcgtatcttc tcgttactag aaaacgccga agaagaaaaa gaacgg
//
LOCUS       DAZKGS010000573          646 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZKGS010000573 DAZKGS010000000
VERSION     DAZKGS010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..262)
                     /locus_tag="ACHOQY_005524"
     CDS             complement(<1..262)
                     /locus_tag="ACHOQY_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681367.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFSIT"
     gene            456..>646
                     /locus_tag="ACHOQY_005525"
     CDS             456..>646
                     /locus_tag="ACHOQY_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681368.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNV"
BASE COUNT          205 a           99 c          112 g          230 t
ORIGIN      
        1 ctgttatcga aaatttatat atgctcatag cagataatat tgcttgctga attatctctt
       61 cagaaatatc cgctatcttt attgacaaat catgtgttag atattttcct ccaagagatt
      121 cttgttcaat ataatttaaa ccagacccca ttgtgatttt aactggatta acggatttct
      181 cttcattttt cttatttttg ttctcctgat caggaactaa tggttgcagt ggtaggctat
      241 tattaatatt tcgtggcttc attttacatt tctctcattt gattagagca aaatccgaat
      301 tcgcagcttc tgataatgtc aaatatcacc ttatggagac attacgtaga atgactatat
      361 ttgttatcgg cacattaggg gtattaattt tagtattgat ggaaggtgat tattgaacag
      421 taacatcgag tcacggtaaa aatgtgaggt atataatgga cattgagttt tcgcagattc
      481 atgaaatggt ctatatgcat gatattgtaa attcggactc gaaaaagaaa ccaaggattc
      541 cgttaaaaaa attccttaat gctgagaagg ttctcacgca aacaacatca tgggcattaa
      601 attcaagatt tgtgaatgtt aattctgtca ataaagtcaa cgtaaa
//
LOCUS       DAZKGS010000574          638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZKGS010000574 DAZKGS010000000
VERSION     DAZKGS010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>638
                     /locus_tag="ACHOQY_005526"
     CDS             <1..>638
                     /locus_tag="ACHOQY_005526"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP9681369.1"
                     /translation="FTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERNLFLF
                     SKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMG
                     GNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLK
                     GGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKW"
BASE COUNT          214 a           91 c          129 g          204 t
ORIGIN      
        1 attcacggaa gataaaaata aaacccttac cttcagatgg acgaaaaaac actttgagtt
       61 ttgtatggat aataaaatct ttttaaatca taaggggaag aagtcgtata aagaaaggaa
      121 tttatttctt ttctcaaaag gtgacaagat aacaattgaa gataatgtta ttgctgaaga
      181 atattcaaca atgcctgtga aaaatttctc cagtgtcggc gctttttcat ttccgacctg
      241 ccatttttca gggaatatac gtataggtcg attttgtagt attgcatcaa atgtaaaaat
      301 aatgggtgga aatcatcctt tgaatcgttt tactactcac atgatgactt ataacggtga
      361 atttgataag tttgctatga gtgaatttga acgtagctgg acattgaaac cattcattac
      421 caaacctgaa aatccgatta taggaaatga tgtctggata ggaaatgatg ttgtattgaa
      481 aggtggcatc gcaataggtg atggtgctgt tattgctgcc aattccgttg ttaccaaaga
      541 tgtgccccca tatgccatag ttgcaggagt tcctgcaaaa ataatcagat ttaggtttga
      601 ttcaaatgtt attgatgaac tgttgagaat aaaatggt
//
LOCUS       DAZKGS010000575          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZKGS010000575 DAZKGS010000000
VERSION     DAZKGS010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..483)
                     /locus_tag="ACHOQY_005527"
     CDS             complement(<1..483)
                     /locus_tag="ACHOQY_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9681370.1"
                     /translation="MGTFEFLSQNTSHTEREHSHFDLKAKKYAGLMIFRDSLRVLPYG
                     RVDNDFFQIEERRSWNAGRYYWSNRRIFGYIGITQSSNKELKDKSGREGFIRNQAARE
                     LKTIISNLLTELADRFFGSRSDDRKELLEQVKREKELRKSAQQQARKSTQKSFSEALK
                     N"
     gene            complement(542..>637)
                     /locus_tag="ACHOQY_005528"
     CDS             complement(542..>637)
                     /locus_tag="ACHOQY_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9681371.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          177 a          146 c          102 g          212 t
ORIGIN      
        1 attcttcaaa gcttctgaaa aacttttttg tgttgatttt cgagcttgtt gttgagcaga
       61 ttttcttaac tctttttcac gcttaacttg ttctaaaagc tctttacggt catcagaacg
      121 tgaaccaaaa aatctatcag caagttcagt taacaaatta gatataatag ttttcagttc
      181 tcttgcagcc tggtttctta tgaatccctc tctccccgac ttatctttca gctctttatt
      241 actggattga gtaattccaa tataaccaaa aatccttcta ttagaccaat aatatcgccc
      301 tgcattccat gaacgtcttt cttctatctg gaagaaatca ttatctactc gaccataagg
      361 taatactctc aagctatccc taaaaatcat tagtcctgca tactttttgg ccttaagatc
      421 aaagtgggaa tgttcacgtt cagtatgtga tgtattttgt gaaaggaatt caaacgttcc
      481 tatctgaagc tcaaatgggc cgccccctgc gtgatcctac ccacgtaata tggacacagg
      541 cctaagcgag gttcttgttt tcaaattgtt ccggactgag gccgccacac caactgtgcc
      601 gccgccaccg attgtaatca cattcgatat aattaaa
//
LOCUS       DAZKGS010000576          636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZKGS010000576 DAZKGS010000000
VERSION     DAZKGS010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>636)
                     /locus_tag="ACHOQY_005529"
     CDS             complement(<1..>636)
                     /locus_tag="ACHOQY_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681372.1"
                     /translation="QGGWYPNRGNHLYAIDALCVNIHDALANSIFDGLENYHKFLYMA
                     PEFLSTAGLNSESVVSKETFVLFIDKFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQ
                     LQGEFYYTLNFEPLFFPNIEEEDGIRYVTSPVVTKLFALLGFIYIRMYSLLDYITKLA
                     IEIENLKTQFSSYVKLTSKNSQYGDKKKVSLNNYKGSLFEQCPFINEIESVR"
BASE COUNT          207 a          117 c          102 g          210 t
ORIGIN      
        1 cgtacacttt ctatttcatt aataaatggg cattgctcaa acaaagatcc tttataatta
       61 tttaagctca ctttcttttt atcaccatat tgagagttct tggatgtgag tttaacataa
      121 gaagagaact gtgtttttaa attttcaatt tctatagcta gttttgttat gtagtcgagc
      181 aaactgtaca tgcggatata aataaatcca agtaatgcaa agagcttagt cacgactgga
      241 gatgtaacat atcttatacc gtcctcctct tcaatgttag ggaaaaacaa gggttcaaaa
      301 ttcaacgtgt aataaaactc cccttgtagt tgcaacactt ccttgctgca ttcctgtatg
      361 ctacttacaa ttttacagca atcaaaaagg taaaggcctt tatttacgtc agtatgccct
      421 ttgaatttat caataaataa aacaaatgtt tcttttgaaa ctacggattc agagttcaaa
      481 cctgctgttg ataaaaactc aggggccata taaaggaact tgtggtagtt ttctaatcca
      541 tcaaaaatgc tattagctag agcatcatga atattaacgc ataatgcatc gatcgcataa
      601 agatgattcc ctctgttcgg gtaccatcca ccttga
//
LOCUS       DAZKGS010000577          636 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZKGS010000577 DAZKGS010000000
VERSION     DAZKGS010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..252)
                     /locus_tag="ACHOQY_005530"
     CDS             complement(<1..252)
                     /locus_tag="ACHOQY_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9681373.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKS"
BASE COUNT          205 a          107 c          115 g          209 t
ORIGIN      
        1 gcttttttca atttgtgaaa gtatgagttc aggagatttt gcttcatatt ctttagtttc
       61 accatcgaca agaaagatat aaccgttctt gtaaagatta tcaatagatg agtatacaga
      121 cgaacgagaa agtgaaattt cttttgctat cttgtaacca ctgctacgac cgtttttcaa
      181 cagactgatg taaacctgtg catcggtgcg agtgaaacca aaatttatca atttatttac
      241 caaatgtatc atagacgcgc tcctgaaatg aaaatgaaat cttaccaatt ttaacattat
      301 taattaacat tttcgaaaaa attgctgagc taaaatgtgg atttatcgta attcatcctc
      361 aagctggtta caggtctaca gtacgtaagc attgtgaact gcgaatctga atgccctcca
      421 actatggtca gaagacatgc tctgacctaa atagtaggct cactgagtaa gcggtgttat
      481 caacggttta ttcatatatg ctctttttta tggatggaat tttgtaactc aattaccttg
      541 ataagaatca gttcgtggag ttaatgacat ttaccaatag acagttcgag cagtaagaag
      601 ccttatggaa ttgtaaggtt taataccgtt attcaa
//
LOCUS       DAZKGS010000578          632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZKGS010000578 DAZKGS010000000
VERSION     DAZKGS010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..511
                     /locus_tag="ACHOQY_005531"
     CDS             <1..511
                     /locus_tag="ACHOQY_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP9681374.1"
                     /translation="NTPINNPVLRSITNEMIVLQYNLSVEHFNLNSSLIYYINNWNLF
                     PLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLSAIPVKINEKARYKPGIASYHNIIT
                     CGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLERITFYAKKNRDELD
                     KISCKWCCD"
BASE COUNT          214 a          103 c          105 g          210 t
ORIGIN      
        1 taatacacct attaacaatc cggtgcttag atctattacc aatgaaatga ttgttctgca
       61 gtacaatctt tcagttgaac attttaatct gaatagttca cttatttact acataaacaa
      121 ttggaattta tttccattgt tctgtttatt cagtggttat cacttttatc gagaaaggtt
      181 tgcagaacgc ggattttttt ataaggttcc tgctgtgcta agggattatt tatcagctat
      241 accggtgaaa ataaatgaaa aggcacgata caaacccggc atcgctagct atcacaatat
      301 catcacatgt ggtttttcaa cgttgtcacc ctatataagg caacaaccat tggcaatgca
      361 gcaacgattt aatttattat ttcctgattt tgttgatcac atacaattgc cgttaccact
      421 tgcatcaact cttttagaga gaattacatt ttatgcgaaa aaaaatagag atgagcttga
      481 taaaatctcc tgcaaatggt gttgtgatta aaagaaaaat ttctgatgga ttgaaataaa
      541 tagtttctct taaagaaagt atccttcttg aaaccagcgc aaaaattcag agcattgagg
      601 taaagagaga agaaaaattt attcaaggtt at
//
LOCUS       DAZKGS010000579          629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZKGS010000579 DAZKGS010000000
VERSION     DAZKGS010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..418)
                     /locus_tag="ACHOQY_005532"
     CDS             complement(<1..418)
                     /locus_tag="ACHOQY_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFP9681375.1"
                     /translation="MNLLNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREV
                     SGNGIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIIL"
     gene            complement(381..>629)
                     /locus_tag="ACHOQY_005533"
     CDS             complement(381..>629)
                     /locus_tag="ACHOQY_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFP9681376.1"
                     /translation="YAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTIYLSITL
                     NPNNKNHIFLYHKKNYICDKLKEIFPDEPIEFSIEYEN"
BASE COUNT          197 a          129 c          102 g          201 t
ORIGIN      
        1 cgagtataat tacattaagt ctgttttctc cattattatt ttctattacc ggataaacat
       61 ctttatttag catcgtaaat tgaacatcat caacagacac atcaccttct ggtaaagcag
      121 tctgcattac ccataagatc atgagtttta tcatatcttc cggtatcagc cggctttcgc
      181 tatacgggaa taactcctgg caccactgaa tgaactgttc ttctgatatc caaaaacgcc
      241 cctcatagcc tgagcatgtt aacgtgaagc ggataccatt accgctgact tcgcgaaaat
      301 atgcatggca agcatgccct tcaggcgtaa tatagaggcg accggttcct atgactgaca
      361 acaatggcaa gtaaaaatca ttaattttca tattcaattg agaattcaat aggttcatca
      421 ggaaagattt cttttaactt atcacaaata taattctttt tatggtataa aaaaatatga
      481 tttttgttat tggggttcag tgtaatagac agataaatgg ttccattact acattttatt
      541 gttaaaacaa ttccgcgtaa ctttccacaa ataatactaa atgaacgctc tccttgttca
      601 aagtagcgtc gcctctgtcc aggagcgta
//
LOCUS       DAZKGS010000580          614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZKGS010000580 DAZKGS010000000
VERSION     DAZKGS010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(401..>614)
                     /locus_tag="ACHOQY_005534"
     CDS             complement(401..>614)
                     /locus_tag="ACHOQY_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681377.1"
                     /translation="KYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYRTL
                     QGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          226 a          114 c           96 g          178 t
ORIGIN      
        1 acgaggtatg tttctggtgg aaaggacagt gggattaaaa agtaagaatt gataaaaaaa
       61 acgccagcaa cacatagtgc cggcggaggg aataccccat ggagaaaatg tgatgcctag
      121 agcatcatga atatataatt aaaaatagtt agcgttgtca atactttcac aaaaataatt
      181 ttcgtagtat agaaagatat ttttatgcat gacctacctg aatttgctcc gggtagaggt
      241 tataaataaa aattgaatca cgacaaacac aatattcata gtatggcgat gcctacgcca
      301 gcaagaatag cgccaataat attgggaata taatagacac cagacgcaca ggcatctcac
      361 tccttaacaa acaacaatca ggatatttac ttttaccaag ctaactgttt acacccaaag
      421 tacacacaat taaccattca ataacaaatg gcaatatcca tagccatacg actttacctt
      481 gtaatgttcg gtatttcttt ataattattc tgggaaatct aacatttatt tttaaaatca
      541 aattatcttg ttgtttaaaa ataagttcac atactttatc atcttcttcc gccatcaaca
      601 tctctgcata ctta
//
LOCUS       DAZKGS010000581          610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZKGS010000581 DAZKGS010000000
VERSION     DAZKGS010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>610
                     /locus_tag="ACHOQY_005535"
     CDS             <1..>610
                     /locus_tag="ACHOQY_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9681378.1"
                     /translation="ARKNGTYSGSQQNTGASDTTAQPEPVKVTADEVNKIMQAANISQ
                     PDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQNQHESERNYQKAEQNSPNAL
                     QNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQE
                     DDPEKMEGAEHPHKENTGGNQHHASDNETGETADHSIKVNGH"
BASE COUNT          202 a          151 c          161 g           96 t
ORIGIN      
        1 tcgcccgcaa aaacggtaca tactcaggtt cacaacaaaa tactggtgca tctgacacga
       61 ccgcacaacc agagccggta aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag
      121 ccaatatcag ccagcctgac gccgataagt tgcttgctgc ctctcgcgga gaatttgttg
      181 cagggattag cgacccgaat gatccgaaat gggttaaggg gatccagacc cgcgattctg
      241 taaaccagaa ccagcatgaa tcggaacgga actaccaaaa agcggaacaa aacagcccaa
      301 atgcgttaca aaacgagcca gaaacgaaac agcctgaacc agtggcgcaa caggaagtgg
      361 aaaaagtctg caccgcctgc ggtcagaccg gcggcggcaa ctgccctgat tgtggcgcgg
      421 tgatgggcga cgcaacatac caggaaacat tcgatgaaga gtatcaggtt gaagttcagg
      481 aagatgatcc ggagaaaatg gaaggcgctg aacatccaca caaggagaac actggcggca
      541 atcagcatca tgccagcgat aatgaaactg gcgagacggc agatcactca attaaggtga
      601 acggtcatca
//
LOCUS       DAZKGS010000582          609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZKGS010000582 DAZKGS010000000
VERSION     DAZKGS010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>609
                     /locus_tag="ACHOQY_005536"
     CDS             <1..>609
                     /locus_tag="ACHOQY_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc5 family transposase"
                     /protein_id="HFP9681379.1"
                     /translation="HDIIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLR
                     HSYDKSRRRGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTD
                     AMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSH
                     GREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQ"
BASE COUNT          200 a          113 c          141 g          155 t
ORIGIN      
        1 cacgatatca ttgccagagt tgtatcctgt atcagtcctg caaaatttca cgagtgcttt
       61 attaactgga tgcgtgactg ccattcttca gatgataaag acgtcattgc aattgatgga
      121 aaaacgctcc ggcactctta tgacaagagt cgtcgcaggg gagcgattca tgtcattagt
      181 gcgttctcaa caatgcatag tctggtcctc ggacagatca agacggatga gaaatccaat
      241 gagattacag ccattcctga acttcttaac atgatggata ttaaaggaaa aatcatcaca
      301 actgatgcga tgggctgcca gaaagatatt gcagaaaaga tacaaaaaca gggaggtgat
      361 tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga ggaaaaattt
      421 ccgctgaaag aattaaataa tccagagcat gacagttacg caatgagtga aaagagtcac
      481 ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact tattgatttc
      541 acgtttgaat ggaaagggct gaagaaatta tgcgtggcag tctcctttcg gtccataata
      601 gcagaacaa
//
LOCUS       DAZKGS010000583          605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZKGS010000583 DAZKGS010000000
VERSION     DAZKGS010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..592)
                     /locus_tag="ACHOQY_005537"
     CDS             complement(<1..592)
                     /locus_tag="ACHOQY_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681380.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQ
                     TSIEMVTLLNTLQPDGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNPFKLNSTNFTNANMFQVN"
BASE COUNT          191 a           94 c          105 g          215 t
ORIGIN      
        1 aattaacctg aaacatgttg gcattagtaa aatttgttga gttcaattta aaaggattta
       61 aaatagtatt agccatatgt ttatctgtga aatcataata atctaaatcg acattacgta
      121 agtccagaga ggatagatca acgatatctc cagttccaca actaaagatt acgttcaacc
      181 tcctgatttc attgtaaata tatttcaatg gacaattaac attgatactg cctgtggata
      241 ttttctggtt ctttaatggt tcattaatgg taagttcgca ggtattgtcc gatcgttgaa
      301 taatttgggc gttataacct aaaaaatcca gacgaagaga cgagtcatta taaagaatta
      361 cattttgtaa gtacgctaat tctttttgaa aatcaccagt gataatgcat tttccatcag
      421 gttgtaacgt gttaagtaaa gtgaccattt caatagaggt ctgttttggt ttttctatac
      481 ctggtgctag tgcttcgagg ttatttcctg ttgggctttc aggaatgtca ttcatttttc
      541 gacggactaa atcactacta atatcattac caattgatga aacagggaac attattttat
      601 ccttt
//
LOCUS       DAZKGS010000584          602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZKGS010000584 DAZKGS010000000
VERSION     DAZKGS010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..255)
                     /locus_tag="ACHOQY_005538"
     CDS             complement(<1..255)
                     /locus_tag="ACHOQY_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681381.1"
                     /translation="MPLITSSISSNVALQSIEILREAARQNLITKDITINGQKVGIHY
                     YQRPDVFLVSGCKDGMLKTLLELGLNGSNESAKRLRSWQIS"
     gene            complement(465..>602)
                     /locus_tag="ACHOQY_005539"
     CDS             complement(465..>602)
                     /locus_tag="ACHOQY_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9681382.1"
                     /translation="IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGM
                     K"
BASE COUNT          172 a          155 c           95 g          180 t
ORIGIN      
        1 ggaaatttgc catgaacgaa gccttttggc tgactcattg ctaccattta accctaactc
       61 taataatgtt tttaacatcc catccttaca accagaaact aaaaaaacgt cagggcgttg
      121 ataataatga ataccaactt tctgcccatt tatagtaata tctttcgtta tcaggttttg
      181 tctggcagct tcacgtaata tttcaatact ctgcaaagca acatttgatg aaatgctgga
      241 tgttatcaat ggcatatgtc atttacccca acaagactaa attttagaaa aaacaactgc
      301 cgggacaact ttcgccccct gtaaccccaa ataagatgtc agatttatcg gcattaattt
      361 atctccctgt tgaattagct cctctatcat ctacttaaca aatatccccc ggacattgca
      421 acacaaaaac cggagccgga ctccggtttt gtgaagctgt caggttactt catcccgcca
      481 atattttccc acgtcccgtc agcacgcagg atttgcagcg gtcttaccac gcactgtatc
      541 tgctttttat ctgcatccag tatcaccacc tgcgtgatta ccctgtcctg ctccggaata
      601 at
//
LOCUS       DAZKGS010000585          591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZKGS010000585 DAZKGS010000000
VERSION     DAZKGS010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..314)
                     /locus_tag="ACHOQY_005540"
     CDS             complement(<1..314)
                     /locus_tag="ACHOQY_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFP9681383.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTL"
     gene            complement(316..>591)
                     /locus_tag="ACHOQY_005541"
     CDS             complement(316..>591)
                     /locus_tag="ACHOQY_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9681384.1"
                     /translation="LSNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANG
                     HGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
BASE COUNT          196 a          116 c           92 g          187 t
ORIGIN      
        1 agtgttttat caggtgcatt attatcaaga tgataatcta caataataag atctgatttt
       61 ctaatccgat ccacatcgaa attaacagaa ccattgtcaa catcacaaat catattttta
      121 gattgaaaaa agctctcaag agtagcggct cgtttagatg agtcaatttt gttgtagtct
      181 aaatcaactt cgttattcaa cgccctgatt gattcagaat acgtcagaaa atcgtcatca
      241 atcatgacaa cggaacgaat tgcattttcg cagaaagttt tctggacaag agaattataa
      301 tttgccactg tcatattaga actccactcc attgaactgg atcacaaaat tagcgccatc
      361 ttttattaaa tagttatcgc cttcatcagg ttctgaatac catattttat gatgtgcaac
      421 agcaaggttt tctcgacata gatacagacc taccccatgt ccatttgctc ttttgctata
      481 aaatagttca aatagtcgcg ggatatcatc ggtatcaatt gccggaccag aatttgctat
      541 gataaccaaa gaattcacaa aaccaatctt tatgagccta ttatttgaca g
//
LOCUS       DAZKGS010000586          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZKGS010000586 DAZKGS010000000
VERSION     DAZKGS010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>590
                     /locus_tag="ACHOQY_005542"
     CDS             <1..>590
                     /locus_tag="ACHOQY_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681385.1"
                     /translation="TYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAI
                     VDNGAHFDGDQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRL
                     TSGSGEGSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPA
                     KPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSET"
BASE COUNT          126 a          176 c          185 g          103 t
ORIGIN      
        1 gacatacggt gtatggggac tctccctgcc gtcactgcgt cgtcgcctgt tccgctgtgt
       61 ctccatccgg gaaaacacgg acggcacctt tgccatcacg gcggtgcagc acgtaccgga
      121 aaaggaagcc atcgtggata acggagcgca ctttgacggc gaccagagcg gcaccctgaa
      181 cagcgtcatc cctccggcag tgcagcacct gacggtggag gtgagcgcag ctgacggcca
      241 gtatctggcg caggcgaaat gggacacgcc gcgggtggtg aagggggtgc gcttcagtct
      301 gcgactgacc agcggaagcg gagaaggcag ccgtctggtg accaccgcca tcaccgcgga
      361 tacagagcat cgttccagtg gtctgccgct cggggaatac accctgacag tcagggcaat
      421 taacagttat ggccagcagg gcgaaccggc caccaccacc ttccggatta acgcgccagc
      481 aaaacccgcc accattgaac tgacgccggg gtattttcag ataacggcgg tcccgcgtct
      541 tgcggtgtat gacccgacgg tacagtttga gttctggttt tcggagacaa
//
LOCUS       DAZKGS010000587          588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZKGS010000587 DAZKGS010000000
VERSION     DAZKGS010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            108..>588
                     /locus_tag="ACHOQY_005543"
     CDS             108..>588
                     /locus_tag="ACHOQY_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HFP9681386.1"
                     /translation="MNTNIGMRYIDVVANGNAGEFYFAYWISSNFIWPCRILDIDMGL
                     DAQVEVYDDQNHSTGMFIGVQVKTTATTLEENPNVSVPMKNIAYWESINDPVVIIRTC
                     LNNNSKEPSLYWKHLPKSELRKYLNRAEEKGYETVTIMFEEDKNSLLKTDKSAWLKIF
                     "
BASE COUNT          201 a           80 c          131 g          176 t
ORIGIN      
        1 ttgtaggttg tttagttgaa gtttaaaagc agcgctttag cagttgattg cgttaaacca
       61 ttgtaatata caaaggggaa atagtattta gccgtggggg agtattaatg aatacaaata
      121 tcggtatgag atatatagac gtagtggcaa atggtaatgc cggagagttt tattttgcat
      181 actggatatc aagtaatttc atctggcctt gccgtattct tgatattgat atggggttag
      241 atgctcaagt tgaagtttat gatgaccaaa atcattcaac tggaatgttt attggtgtgc
      301 aggtgaaaac aaccgcgact acgctagaag aaaaccctaa tgtttctgta cccatgaaaa
      361 atatagctta ttgggaaagc ataaatgatc cagttgttat tattcggacc tgtcttaata
      421 acaattcaaa agagcctagc ttgtactgga agcatcttcc gaaaagtgaa ttaagaaaat
      481 atttaaatag agccgaggaa aaaggttatg aaacagtaac cataatgttc gaggaggata
      541 agaactcatt gctgaaaacc gataagtcag catggctaaa gatatttt
//
LOCUS       DAZKGS010000588          586 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZKGS010000588 DAZKGS010000000
VERSION     DAZKGS010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..550)
                     /gene="waaH"
                     /locus_tag="ACHOQY_005544"
     CDS             complement(<1..550)
                     /gene="waaH"
                     /locus_tag="ACHOQY_005544"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP9681387.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKF"
BASE COUNT          160 a          153 c          126 g          147 t
ORIGIN      
        1 taaatttaat gttattttta acaataacat cacggcgata aacccccatc cagacaacgt
       61 gagtccaacg gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacgccgg
      121 ttgagcgaag gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc
      181 agtcagcgtt gcactgcgcc acgtcgaggt catcctctaa cgccatggtc atcagcgttt
      241 cgtacatggt gggatagacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg
      301 ccacctcgat cccacgatta cgcgctaccg atgcgccagc attcgcctga tgcaacaaac
      361 gaacgtgcgg atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac
      421 catcgttaat aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac
      481 aagtgcggaa atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc
      541 tgttcatcat aaattaccga cgacctctga agttatcctg acgttt
//
LOCUS       DAZKGS010000589          585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZKGS010000589 DAZKGS010000000
VERSION     DAZKGS010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..438
                     /locus_tag="ACHOQY_005545"
     CDS             <1..438
                     /locus_tag="ACHOQY_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9681388.1"
                     /translation="PGAPISNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEDDYEEK
                     LLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQ
                     FPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            410..>585
                     /locus_tag="ACHOQY_005546"
     CDS             410..>585
                     /locus_tag="ACHOQY_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681389.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIA"
BASE COUNT          172 a          125 c          146 g          142 t
ORIGIN      
        1 ccgggtgccc cgatatcaaa taagcgaggg caactggatc tggatgcctg gcgcgatttt
       61 tacatatcgt atctgaggag aagtaaaaac gatgtgcctg atggcgatag cgaagacgac
      121 tatgaggaaa aattgcttat tgccagatgg gaactgacag cagaacaggc tgttacacag
      181 cagttaaaaa atgaggtgtc aaaaggaaaa cttattgaca ccgggttctg tatttttgcc
      241 ctcagtaagc tggcaatggc gttatccagt acgcttgatt ccatcccttt atccatgcaa
      301 cgacagtttc ctgatttaac accgcgccat cttgaccatc tgaaaaccct tattgcgaag
      361 ggggcaaatc agtgtgcgcg ggcgggggat aaattaccgg atttactcga tgaatatatc
      421 agagcaacaa ctgaataata tgatgagcgc tgtcacaact gcattacagc ccctgataag
      481 ggcattgccg gtgacgccag ttgaatgggc tgatcaaaat tattatctgc ctaaagaatc
      541 ttcatatggt gagggcgaat ggaaaacgct gccattccag atcgc
//
LOCUS       DAZKGS010000590          582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZKGS010000590 DAZKGS010000000
VERSION     DAZKGS010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..532
                     /locus_tag="ACHOQY_005547"
     CDS             101..532
                     /locus_tag="ACHOQY_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9681390.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTV"
BASE COUNT          204 a          107 c           80 g          191 t
ORIGIN      
        1 aaccctattc cctcaatgtt aagcaaatat aacaattact cttattttaa attttacacg
       61 taatatttac cctaaatgat tttagagtac aggtgggcat atgcaagtct tttccagtga
      121 tgtctacttc actgtaggca ctaatgcatt attagcatcc caaaaagaat attacagcga
      181 tttagttgcg ttggttgatc ttggacattc gttcgttgtt attgatgagc atcaacatcg
      241 aaatctcaaa ccaaatacag aacctgtaaa catccttttg agcaacaatt tcatcaggat
      301 caataagaat attacattat ctgatttaac gcatttcctg gtttcaaatc ttcatacaca
      361 aaatgtgtat tccacacaag aaccacttac ccatgatgag atagatatcc tgcggttatg
      421 tgtttcttac tcgttaaaac aaatagccat tattaaagga attgattata aaacaatatc
      481 atatcacaaa ataagagccc taaataaact taacataaaa ggtactgttt agttgtttat
      541 agcactctgt gaatgggata aacattattt taaactccaa tc
//
LOCUS       DAZKGS010000591          580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZKGS010000591 DAZKGS010000000
VERSION     DAZKGS010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..559
                     /gene="ybeT"
                     /locus_tag="ACHOQY_005548"
     CDS             5..559
                     /gene="ybeT"
                     /locus_tag="ACHOQY_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9681391.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
BASE COUNT          186 a          129 c          123 g          142 t
ORIGIN      
        1 acatatgaat aaaaaattaa tgtatatatt cgcaatattt atagttgcag caattacctg
       61 tattagccac cccaagaaaa caacgttgcg tgataaagcc atggtgaatt atgcctttga
      121 ttatttaagc tcaccgggca gtctaccgtt taccacggcg gccacggagc tttccgcgat
      181 tcatggtcac tcaacgtcgc aatatcgcct tggagaattt tatcttcatg gtagcgacgg
      241 taaaccactg gattatacac aggcgagata ctggtatgag caatcagcag aacaggaaaa
      301 tccgcgcgca caaagcaaac tggggtggat ctacctcaaa ggtctggggg tcaaacccga
      361 cacccgtaaa gcaattctct ggtataagga agcagctgaa caagggtatg ctcatgctca
      421 atatacttta ggtttgatct acagaaatgg ctcaggtatt aatgttaacc attatgaatc
      481 tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa gattacttgc
      541 cgggcttccc gcacattaac gtcgactgtc aaccgaacag
//
LOCUS       DAZKGS010000592          574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZKGS010000592 DAZKGS010000000
VERSION     DAZKGS010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..>574
                     /locus_tag="ACHOQY_005549"
     CDS             3..>574
                     /locus_tag="ACHOQY_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681392.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTS"
BASE COUNT          142 a          156 c          177 g           99 t
ORIGIN      
        1 caatgggcaa aggtggcggc aaggcgcaca cgccggtaga ggcaaaggac aatcttaagt
       61 ccacgcagat gatgagcgtg attgatgcga ttggtgaggg accgatagaa ggtccggtga
      121 agggactgca gagtattctg gtgaacaaaa ccccactaac ggacacggac ggcaatcccg
      181 tgatacacgg tgtgaccgcg gtctggcgtg ccggggagca ggagcagaca ccaccggaag
      241 gctttgagtc ctccggagct gaaaccgcac tgggcgtgga agtgacgaaa gcaaagccgg
      301 tgacgcgcac cattacgtcc gcgaacattg accgcctgcg ggtcaccttc ggggtgcagt
      361 cactgttgga gaccacctca aagggcgacc gtaatccctc ttctgtccgc ctgctgattc
      421 agttacagcg taacggtaac tgggtgacag aaaaggacgt caccattaac ggcaagacca
      481 cctcacagtt cctggcctcg gtgattctgg ataatctgcc tccccggccc tttaacatcc
      541 ggatggtcag ggagacagcg gacagcacct cgga
//
LOCUS       DAZKGS010000593          568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZKGS010000593 DAZKGS010000000
VERSION     DAZKGS010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..195
                     /locus_tag="ACHOQY_005550"
     CDS             <1..195
                     /locus_tag="ACHOQY_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681393.1"
                     /translation="GYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLSTSFNAGSKRF
                     VIYQSAQDKNLQVDIVRTRI"
     gene            281..439
                     /locus_tag="ACHOQY_005551"
     CDS             281..439
                     /locus_tag="ACHOQY_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9681394.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT          157 a           78 c          133 g          200 t
ORIGIN      
        1 ggatattaca aaccacagtt taagaaagaa tatgaaatga ttaattataa tgatggttgg
       61 tattatgttc ttgctcgtta tgataattgt ctggttttgt ctacttcttt caatgcaggt
      121 agtaaaaggt ttgtcattta tcaatcagca caagataaga atcttcaggt tgatattgta
      181 aggaccagaa tttaattggc tgcataaata atattttaag ttgcaagttg gctattcgta
      241 ggaatagaac cttaggcatg ctgaatgcgt tttctgaaca ttgttttata aactgtgtct
      301 gcttgctgtt gtgatcctgc ttttagtgat ggtgatgatg gatttcacca gcaggataat
      361 gttggtactg actgatggcg ctctggtctg cggcattgtg gtattgctgt ggccgatgat
      421 gaaagaacag aatgaataat tcttgacttt tttgtttact gtttattaaa aaatcaaccg
      481 catggtgaat cctccttgga ggggctaaat gatcgagttt taagggcacg tagcgagttc
      541 tgtttgatca ttgcagaact tagcggga
//
LOCUS       DAZKGS010000594          567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZKGS010000594 DAZKGS010000000
VERSION     DAZKGS010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>567
                     /locus_tag="ACHOQY_005552"
     CDS             <1..>567
                     /locus_tag="ACHOQY_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP9681395.1"
                     /translation="DKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYYYIQLRGAF
                     LSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFLPNSKNQTN
                     NRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIAT
                     ASILLTKRSNVDTYTEINSYLGKLRGQQ"
BASE COUNT          214 a           94 c           94 g          165 t
ORIGIN      
        1 ttgataaaag cactccatta tttattaatt ccattaacaa taatgaacaa ttatttatgg
       61 gatttgactt ctatcgaata aatagatttg gtagattgga aaagtattat tatatacaac
      121 taagaggcgc ttttttatcg gctattcatc accagatcat tgaaaaccaa ctggatacag
      181 aaacaataac tattagttat gaatttatcc tctgtcaaca tcttatcgca aataccgagt
      241 tcagctattt ggcactccct gaaaattata accgtttgtt tttaccaaat tcaaaaaacc
      301 aaacaaataa tcgtttcaaa acgttaaaca gcaaagctat tggcaggcta cttgctgctg
      361 gtggcgtata caatgggaac attgaaggat tcagagatac tgcggaaaaa ctgggtggag
      421 atgcaataaa aggctatgat caaatactaa atgaaaaaac agcgggcata gcgatagcaa
      481 cagcatctat tcttttaaca aagcgttcta atgttgatac atatacagaa ataaatagtt
      541 acttaggcaa acttagaggt caacaaa
//
LOCUS       DAZKGS010000595          562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZKGS010000595 DAZKGS010000000
VERSION     DAZKGS010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>562)
                     /locus_tag="ACHOQY_005553"
     CDS             complement(<1..>562)
                     /locus_tag="ACHOQY_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP9681396.1"
                     /translation="SAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPEAYFRM
                     SPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHKFRCVP
                     HLTGRRFEHGVTDCYTLFRDAYHLAEIEMPDFVREDDWWRNGQNLYLDNLEATGLYQV
                     PLSAAQPGDVLLCCFGSSVPNHAAIY"
BASE COUNT          131 a          199 c          138 g           94 t
ORIGIN      
        1 cagtaaatag cggcgtgatt cggcaccgat gaaccaaaac agcacagcag cacatcgccc
       61 ggctgcgccg ctgacaacgg cacctgatac agccctgtgg cctccagatt atccagatag
      121 agattctgac cgttacgcca ccagtcatcc tcacgcacaa aatccggcat ctcaatctcc
      181 gccagatgat aagcatcccg gaacagtgtg taacagtccg tcacaccgtg ctcaaagcgc
      241 cgcccggtga gatgcggcac acagcggaac ttatgaatct caccccggca gaccagccac
      301 cacggcaaat cactctgcac ctgcagccgc cggtcggcct cactcagcca gggcagacca
      361 ccggggtggc tgtggaccag tgccacaatc tccccctgca tttctgcctg cagccagtct
      421 tccggcgaca tacggaaata cgcctccggc tcaccggaga tattcacgca ggggaaatat
      481 ctttccccct ccggcgtgct taccacgaag ccgcacgact ccgctggcgc acatcgccgg
      541 gcgtgcgcca gaatcgctga tt
//
LOCUS       DAZKGS010000596          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZKGS010000596 DAZKGS010000000
VERSION     DAZKGS010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>561
                     /locus_tag="ACHOQY_005554"
     CDS             <1..>561
                     /locus_tag="ACHOQY_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9681397.1"
                     /translation="VDVGKQKFVQAIRELPDFGGYLFAAPPAPIVPEEMYWQDAPVEG
                     SSKAAAYATGWNDCREAMFQSGNFRENKDSSTNNFRKIPEASTSSPVTPALLPGGFTI
                     EEAKELHEDLARSHISKALSGEKMKKKDRDADLRWIHGVIVQAAWFVKASLEQNALSG
                     NYPVTPDSWISCSERMPDTKTAVLVAR"
BASE COUNT          157 a          128 c          153 g          123 t
ORIGIN      
        1 gttgacgtag gcaaacaaaa attcgtacag gccattagag agcttcctga ctttggcgga
       61 tatctatttg ccgcccctcc agcgccgata gtgccggaag aaatgtattg gcaggatgcg
      121 ccagttgaag gcagcagcaa agcggctgca tacgctacag gctggaacga ttgccgcgaa
      181 gccatgtttc agtccggaaa ctttcgggaa aataaagatt cgtcaaccaa taattttcgg
      241 aaaatcccgg aagcgtcaac cagctctccg gtaactccgg ctcttctgcc tggtggtttc
      301 accattgagg aggcgaagga attacatgaa gacctggcac gcagccacat aagcaaggcc
      361 ttaagtggcg aaaagatgaa aaagaaagat cgcgatgctg atttgcgctg gattcatggc
      421 gttatagttc aggcagcgtg gtttgtaaaa gcatcactgg agcagaatgc actatcgggc
      481 aactatccgg taactccgga tagttggata agctgtagtg agcgaatgcc ggataccaaa
      541 acagccgttc ttgttgccag g
//
LOCUS       DAZKGS010000597          560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZKGS010000597 DAZKGS010000000
VERSION     DAZKGS010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>560
                     /locus_tag="ACHOQY_005555"
     CDS             <1..>560
                     /locus_tag="ACHOQY_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9681398.1"
                     /translation="RVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKR
                     VVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFY
                     YNHHSQLTSATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDAT
                     GSRKTMTWSRYAQLLSFTDCSGYVTR"
BASE COUNT          143 a          163 c          164 g           90 t
ORIGIN      
        1 gcgggtgaca gaacagctaa acccggcagg cttaagctac acgtatcagt atgagaaaga
       61 ccacatcacc atcaccgaca gcctggaccg ccgtgaagtg ctgcacacgc agggcgaagc
      121 cgggctgaag cgggtggtga aaaaggaaca cgcggacggc agcgtcacgc agagtcagtt
      181 tgacgccgtg ggcaggctca ggacacagac ggatgccgca ggccggacaa cagaatacag
      241 cccggatgtg gtgacgggcc tcatcacgcg catcaccacg ccggatggca gggcatcggc
      301 gttttactat aaccaccaca gccagttaac gtcagccacc gggcctgacg ggctggaaat
      361 acgccgggaa tatgatgaat tgggccgtct gattcaggaa actgcccctg acggcgatat
      421 cacccgctac cgttatgata atccacacag tgacttaccc tgcgcaacgg aagatgccac
      481 cggcagccgg aaaaccatga cgtggagccg ttacgctcag ttgctgagct tcaccgactg
      541 ttccggttat gtaacccgtt
//
LOCUS       DAZKGS010000598          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZKGS010000598 DAZKGS010000000
VERSION     DAZKGS010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>549)
                     /locus_tag="ACHOQY_005556"
     CDS             complement(<1..>549)
                     /locus_tag="ACHOQY_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP9681399.1"
                     /translation="PLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQH
                     LNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPGLDIRIPYATDP
                     AGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDER
                     THHYHYDSQHRLVFHTRIQHGEP"
BASE COUNT           69 a          172 c          180 g          128 t
ORIGIN      
        1 tggctcgcca tgctgtatcc gcgtgtggaa caccaggcgg tgctggctgt cgtagtggta
       61 gtggtgggtc cgctcgtcgt ccgtccgtat cacacccgtc gggatgcggt ccgtcttctc
      121 cgtcagtctg ccgtattcat catagtggta aacatagtgc gcatcctctg cgatgcggtt
      181 atccggccac gccgtgagcg tgctgtccgg gtgtagctcc gggtccggca ggcggtttcc
      241 cgccgggtcg gtggcatacg ggatgcggat gtccaggccc ggtgcgaggg tgcgcacgct
      301 ctccagcctg cccgtggcac tgtagccgta ttcccgcgtc tgtcgcgggc cgctgatgcg
      361 caccaggtcg ccgttgtcat tccacccgta gtcacggtca tacaccaggc tgttcaggtg
      421 ctggctctgt aactggcctg cgggggtgta tgtgctggtc agttcatatg cggcattact
      481 gcctgccctg ctgccgaagc tgcgcaccgt ctcacggtgc agcctgtccc gcgtatactc
      541 gaccagcgg
//
LOCUS       DAZKGS010000599          548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZKGS010000599 DAZKGS010000000
VERSION     DAZKGS010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>548
                     /gene="espF"
                     /locus_tag="ACHOQY_005557"
     CDS             <1..>548
                     /gene="espF"
                     /locus_tag="ACHOQY_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9681400.1"
                     /translation="QAVSTLGRHITSAASRVSSAGLSGFSVSPQAVRLNPIRAQSPFS
                     PGTSNINARTTFNVSSPATSFTPSRPAPPPPTSGQASGASRPLPPIAQALKDHLAAYE
                     KSKSLDTSSLKPSRPAPPPPTSGLVSGSSRSLPPIAQALKDHLAAYEKSKSLDTSGLK
                     PSRPAPPPPTSGQASEASRPLPP"
BASE COUNT          119 a          170 c          131 g          128 t
ORIGIN      
        1 caagctgttt ctacactagg acggcatatt actagtgcgg caagccgggt aagctctgcg
       61 gggttatctg gcttttcagt gtcccctcag gctgttcgtc tgaatccgat tagggctcaa
      121 tctccttttt ctcccggtac gtcgaatatc aatgcgagaa cgacttttaa tgtgagtagc
      181 ccggcgactt catttactcc ctctcgtccg gccccaccgc cgccgacaag tggacaggct
      241 tccggggcat cccgcccttt accgcccatt gctcaggcat taaaagatca tttagctgcc
      301 tatgaaaagt cgaaaagtct tgatacttca agccttaagc caagccgccc ggcaccgccg
      361 ccgccgacaa gcgggttggt atctgggtca tcccggtctt taccgcccat tgctcaggcg
      421 ctaaaagatc atttggctgc ctatgaaaaa tcgaaaagtc ttgatacttc aggtcttaag
      481 ccaagccgcc cggcaccgcc gccaccgaca agtggacagg catccgaggc atcccggcca
      541 ttaccgcc
//
LOCUS       DAZKGS010000600          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZKGS010000600 DAZKGS010000000
VERSION     DAZKGS010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>544
                     /locus_tag="ACHOQY_005558"
     CDS             <1..>544
                     /locus_tag="ACHOQY_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681401.1"
                     /translation="PEALRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASKAEA
                     SAANADTSAEDASESARQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSK
                     KTAESAAGNASRDATTATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGDP
                     GPAGPQGPKGDKGERGDTGPA"
BASE COUNT          160 a          140 c          184 g           60 t
ORIGIN      
        1 ccggaggcac tgcgtcgttt tgaactgatg gtggaagaag cagcgcgtca cgcagaggag
       61 gcgaagaaga atgccggaga agcagagacg tccgcgagga atgccggcat atcagccagt
      121 aaggcggaag cgagcgccgc aaatgctgat acttcagcag aggatgcatc ggagtcagcc
      181 cggcaggcgg cagaaagtgc agcctctgca aagaagtcag aggaagcgtc ctcgtcctca
      241 gcctctgagg ccgctcaaaa agccagtgag tcattacaaa gtgcaacaga tgccgagttg
      301 tcaaaaaaga cggcagaaag tgcagccggt aatgcatcca gagacgcaac gacagcaaca
      361 gaaaaagccc gggagtcagc agaaagcgca cagtcagcgg aacaaagcag gatagcggcg
      421 gaagaagccg taaaccgaat ccccaccgtg gtggggccac ccgggccaaa gggagatccg
      481 gggcccgcgg gtcctcaggg gccgaaggga gataaaggag agcgtggcga caccggcccg
      541 gcag
//
LOCUS       DAZKGS010000601          543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZKGS010000601 DAZKGS010000000
VERSION     DAZKGS010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            171..323
                     /locus_tag="ACHOQY_005559"
                     /pseudo
     CDS             171..323
                     /locus_tag="ACHOQY_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(348..>543)
                     /locus_tag="ACHOQY_005560"
     CDS             complement(348..>543)
                     /locus_tag="ACHOQY_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681402.1"
                     /translation="RDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWI
                     LFTTIMLSAIPSAKMSLSAH"
BASE COUNT          166 a          106 c          107 g          164 t
ORIGIN      
        1 cttggttata tttaagctca atatcaattc gagaacaaat gctagggtaa aaaaatattt
       61 aaaaaagaag taaacttcac attttttcat tacatctctg aagagaacct cgttaatgat
      121 tggtcttgct cattgttaat aacaaaatgt tgaatttacc gattaggaaa ctttttccta
      181 cgcggattgc ctccagctat ggcatgaact ttgagtttat gccagaactg aagtggggct
      241 tcggctaccc tggcgcgatt atctttatga tcctcgcggg cctgacaccg tatctgtact
      301 ttaagcgtaa gaactggctg taactatacg atgtcgctgt gagtaagtta gtgtgctgac
      361 agcgacattt tagccgaggg aatcgcagat aacataattg ttgtgaataa tatccatgcc
      421 acgttgagtt ggacgcttaa tgactcacat ctcacccctt caattgctgc agagataata
      481 gaaaaaagtg tcaagcccaa tgaaatcaat acaaagtaga ttgccttaca tccagtatct
      541 ctt
//
LOCUS       DAZKGS010000602          532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZKGS010000602 DAZKGS010000000
VERSION     DAZKGS010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /locus_tag="ACHOQY_005561"
     CDS             <1..517
                     /locus_tag="ACHOQY_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9681403.1"
                     /translation="VFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSIN
                     MPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKEIA
                     SSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDV
                     LSSLVMRVAQD"
BASE COUNT          163 a           91 c          118 g          160 t
ORIGIN      
        1 tgtcttttct tcttttttta tgagcgctgg agtatttgct gcagacgttg ataccggaac
       61 attaactatt aaggggaata ttgcagaatc tccgtgtaaa ttcgaagcgg gtggtgattc
      121 agtaagtatt aatatgccga ctgtaccaac cactgtcttt gaaggtaaag ctaaatattc
      181 tacctatgat gatgcagtcg gtgtaaccag cagcatgtta aaaattagct gcccgaaaga
      241 agttgctggt gtaaaactct cgttgattac caacgataaa ataaccggta acgataagga
      301 gatagccagt agcaacgata ccgtgggtta ctatctctat ttaggtgata acagcgatgt
      361 cctggatgtt tctgcacctt ttaacattga gagttataaa acagcggaag gtcaatatgc
      421 tattccgttt aaagcaaaat acctgaaact gacagataac tcagtgcaat caggtgatgt
      481 gttatcttct ctggttatgc gtgtggcgca ggattaataa ataaaattgt aa
//
LOCUS       DAZKGS010000603          529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZKGS010000603 DAZKGS010000000
VERSION     DAZKGS010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>529)
                     /gene="wzy"
                     /locus_tag="ACHOQY_005562"
     CDS             complement(<1..>529)
                     /gene="wzy"
                     /locus_tag="ACHOQY_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen polymerase"
                     /protein_id="HFP9681404.1"
                     /translation="LVIVLIITTLFIINRQNQDYEAYVDIFNVNELYAEIGYRWLIYG
                     VKYLGGTHEVIIGLLGLFLGTTFLRLIQYSKYTAFGLLLYMLCPMPIDIVQIRNTFLF
                     LFVINSLIELEKEHKFNSLCFVFMAPLFHSLGIVYVLAWVIIQFRTWRGYNKLMVFGL
                     VLSFIVVPLLIKMLIL"
BASE COUNT          218 a          100 c           73 g          138 t
ORIGIN      
        1 ataaaattaa cattttaatc aagagtggca ctacaataaa agacagcacc agtccaaaaa
       61 ccattaactt attataaccc ctccaggtac gaaattggat tattacccac gccaatacat
      121 aaacaatgcc taaactatgg aaaagaggtg ccataaagac aaaacaaaga gagttaaatt
      181 tatgctcttt ttctaactcg ataagagaat ttatgacaaa taaaaaaaga aatgtattcc
      241 tgatttgaac aatgtcaata ggcattggac acaacatgta aagcagcaaa ccaaatgctg
      301 tatacttact gtattgtatt aatcgtagga atgtggtccc aaggaataaa cccagcaagc
      361 caattatgac ttcatgggta ccgcctaaat acttaacacc ataaattaac cagcgataac
      421 caatctcggc ataaagttca ttgacattaa atatatcaac atacgcttca tagtcttgat
      481 tctgcctatt aataataaat aaagtagtaa taataagcac aattaccaa
//
LOCUS       DAZKGS010000604          529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZKGS010000604 DAZKGS010000000
VERSION     DAZKGS010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..519
                     /gene="espW"
                     /locus_tag="ACHOQY_005563"
     CDS             <1..519
                     /gene="espW"
                     /locus_tag="ACHOQY_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP9681405.1"
                     /translation="YKNNGKDNAKPNLGERDVRMFENISPDDTDKITGRKFSELGEYL
                     YSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYSDEVLQRNGTLIPTPLHEEKS
                     IPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSPRNYGLH
                     HNIKTWDPLVRN"
BASE COUNT          208 a          100 c           90 g          131 t
ORIGIN      
        1 tataaaaaca atggaaaaga taatgcaaaa ccaaacttag gagagcgaga cgtaagaatg
       61 ttcgaaaata ttagtcctga tgatacagat aagataacag gaaggaaatt ttcagagtta
      121 ggtgagtatc tttattcagg aaatgtaata aaactcagtc agttatcaat tcgttactta
      181 cccaatatca actcaatctc attaatagag acaaaacaga gtttgttgct acatcgatta
      241 tattcagatg aagtacttca gagaaatgga acacttatcc cgacaccact acatgaagaa
      301 aaatcaattc cagctgacaa tataaaaaca atgctcaaca acataccgac ttacaaaatg
      361 ttaccgccat tcacagaaac acaaggtaat tgttcttctg gcgcagccac gtttttacgc
      421 aaatcaggcg ccgaagaaaa agatattctt gcatgtagcc cccgaaatta tgggctgcat
      481 cataacataa aaacatggga ccccttggtt agaaattaag gatccaaat
//
LOCUS       DAZKGS010000605          525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZKGS010000605 DAZKGS010000000
VERSION     DAZKGS010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>525)
                     /locus_tag="ACHOQY_005564"
     CDS             complement(<1..>525)
                     /locus_tag="ACHOQY_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681406.1"
                     /translation="IDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEY
                     CLDDENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLR
                     AILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVN
                     LQSDNKIQGQGGFLS"
BASE COUNT          171 a          122 c           68 g          164 t
ORIGIN      
        1 gataaaaaac caccctgccc ctgaattttg ttgtcagatt gtagattaac gccgaataat
       61 gtctttacat cttccctgag atcattgaat gccgtatcaa caagagcctc gatcgtttgt
      121 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
      181 gcccatgaat actcatgttc ttttaaaacc agtatggccc ttaacagaca atagccatcg
      241 ccatctactg gagcgtattt taatccataa gttttattaa tttcactctg aataaaagat
      301 ataccagaca aatcaatacc agtatctaaa tcagatattc ccggcgtatt tatccattct
      361 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
      421 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
      481 gactctgaac ctctgcccgg caaggacact ctaacaatat caata
//
LOCUS       DAZKGS010000606          516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZKGS010000606 DAZKGS010000000
VERSION     DAZKGS010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>516)
                     /locus_tag="ACHOQY_005565"
     CDS             complement(<1..>516)
                     /locus_tag="ACHOQY_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFP9681407.1"
                     /translation="DKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECKE
                     IGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQLTQSNFRE
                     CQIRNSNFDNCYLYASHFTRAEFLSAKEVSFIKSNMTAVMFDHVRISTGNFKDCITEQ
                     LELTIDYSDIFG"
BASE COUNT          178 a          100 c           75 g          163 t
ORIGIN      
        1 ccaaatatat ctgaataatc aatagttaat tccaattgtt ctgtaatgca atctttaaaa
       61 ttccctgtcg atattcgcac atgatcaaac ataacagctg tcatattcga tttaataaat
      121 gatacttctt tggcagacag aaactctgct ctggtgaagt gcgaagcgta aagataacaa
      181 ttatcgaagt ttgaatttct aatctgacat tcgcgaaaat tactctgagt aagttgactc
      241 tgcgagaagt caactgtatc aagaatattt ttgctgaaat cgacacattc accactcgct
      301 ttgataaaaa tcacattgtg taaacgtcga catctaaata tgacattatc aactattgca
      361 gtagtaaaat tgcaatcccc aatttcttta cattcgaatg ttacatgttg tagatttgat
      421 gataaaaaag acaccttcga tataacgtca cctttaaaat gacaacttgt taaatccaaa
      481 tcagacaggt cgatataccc ctgcgcatct ttatcc
//
LOCUS       DAZKGS010000607          515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZKGS010000607 DAZKGS010000000
VERSION     DAZKGS010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..>515
                     /locus_tag="ACHOQY_005566"
     CDS             182..>515
                     /locus_tag="ACHOQY_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFP9681408.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQRQVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIF"
BASE COUNT          114 a          135 c          118 g          148 t
ORIGIN      
        1 agcttgtgat tatttttttg actgaacaga cacccgtttc tgtaaagtaa tatttaaaca
       61 aaattctcac ataaccatag cattttctgt tccttcccta ataacgtctg aagtcgtccc
      121 gcttatcaca ttatccgcag ttgtacggta cggatcatta ttcattttac cggaggttgc
      181 tatgtcaacc ggattacgtt tcacactgga agtggacggc ctgccgccgg acgcttttgc
      241 ggtggtttcc tttcatctga accagtcact ctcttcgctc ttttccctcg atctctccct
      301 ggtcagccag cagtttctct cccttgaatt tgcgcaggtg ctggacaaaa tggcctacct
      361 gacgatatgg cagggcgatg aattacagcg ccaggtgaaa ggcgtggtga cctggtttga
      421 actcggggag aacgacaaaa accagatgct gtacagcatg aaggtgcacc cgccgctgtg
      481 gcgtgccggt ctgcgccaga acttccgtat cttcc
//
LOCUS       DAZKGS010000608          513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZKGS010000608 DAZKGS010000000
VERSION     DAZKGS010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            218..>513
                     /locus_tag="ACHOQY_005567"
     CDS             218..>513
                     /locus_tag="ACHOQY_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9681409.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTG"
BASE COUNT          151 a           98 c          141 g          123 t
ORIGIN      
        1 tccggcgatc cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat
       61 tttccgggat ccatgtccgg tttctcttca agttaactat atgaaaaata taaaaacagg
      121 tcttctgtga accggacatg aacaaaaaac agacatgtaa accggacatg accggttttg
      181 ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg gctacgcaga ctgaagttgc
      241 caggcattta agtctgaccg atcgccagct tcgcagattg cagaaattac cgggtgcccc
      301 ggtctcgaat aagcgagggc aactggatct ggatgcctgg cgcgattttt acatatcgta
      361 tctgagaaga agtaaaaacg atgtgcctga tggcgatagc gaagaagact atgaagagaa
      421 attgcttatt gccagatggg aactgacagc agaacaggct gttacacagc agttaaaaaa
      481 tgaggtgtca aaaggaaaac tgattgacac cgg
//
LOCUS       DAZKGS010000609          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZKGS010000609 DAZKGS010000000
VERSION     DAZKGS010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>506
                     /locus_tag="ACHOQY_005568"
     CDS             <1..>506
                     /locus_tag="ACHOQY_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681410.1"
                     /translation="DPEVLKQVVNDAVGSIPVPADGKSITPDDVRPMLEQMVKEAVSH
                     IPVPRDGRDYDPEVLKQAVNDAVAKIPVPADGKSITPDDVRPMLEQMVKEAVSHIPAP
                     RDGRDYDPDVLRKAVLEAVNALPAPQDGRDATALEVLPAIDDQKSFPRGTYATHLGGF
                     WRAYEKTHG"
BASE COUNT          114 a          125 c          165 g          102 t
ORIGIN      
        1 gatccggaag tactgaagca ggtggtgaat gatgctgtcg ggagcattcc tgtaccggca
       61 gacggcaaaa gtatcacccc cgatgatgtg cgtccgatgc ttgagcagat ggtgaaagag
      121 gcggtaagcc atattcctgt tccgcgtgat ggtcgtgact acgatccgga agtactgaag
      181 caggcggtga atgatgcggt cgcgaaaata ccggtgccgg cagacggcaa aagtatcacc
      241 cccgatgatg tgcgtccgat gcttgagcag atggtgaaag aggctgtaag ccatatccct
      301 gctccgcgtg atggtcgtga ttacgatccg gatgttctgc ggaaggcggt tctggaggcg
      361 gtgaatgccc tgccggctcc gcaggacggg cgtgatgcca cggctctgga agtgctcccc
      421 gctattgacg atcaaaaatc ctttccccgg ggcacgtatg ccacacacct gggaggattc
      481 tggcgggcgt atgaaaaaac gcacgg
//
LOCUS       DAZKGS010000610          506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZKGS010000610 DAZKGS010000000
VERSION     DAZKGS010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          167 a           72 c          109 g          158 t
ORIGIN      
        1 gttaatataa atcaaattgc atttgattat ataaaagaca tgattgtaaa tgtttcaaaa
       61 actgaaagat tggatggtta ttttcgtgtg aatgatgatg aagataattt attcttagaa
      121 tggattgtaa aagatggtta tttagaggaa ataattttgt ctggttcata aattcatctt
      181 ctttgttgag aagattaact gcctaggtga aattatcctc tatgaccggg acgctgcagg
      241 ccacctggca gaagtcatca cgcaggaaga caagtcgctg tatccctttg aaaaatccgg
      301 caggctaatg ggcatcttca gcccaggcgg cacatcacag cgaatttgaa ggcaaatgcg
      361 aaaattagtg atcttgaagt tcatacagta ttggtaaaaa caggcgaagc ttttccaatg
      421 tgtaagaatt ttcaaatttc aatatttggc gctgttgtta caagcgggtg attatatgtg
      481 tgaattaatt aatgaagcat caaaat
//
LOCUS       DAZKGS010000611          504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZKGS010000611 DAZKGS010000000
VERSION     DAZKGS010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>504
                     /locus_tag="ACHOQY_005569"
     CDS             <1..>504
                     /locus_tag="ACHOQY_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9681411.1"
                     /translation="YAWYQGDSTLSAFNKTLVLSGNQSGLTADRMLTLSRAGQAAGLT
                     FNQASESLAALVNAGVRGGEQFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGL
                     IAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENMGTLET
                     WADRTARA"
BASE COUNT          103 a          132 c          167 g          102 t
ORIGIN      
        1 gtacgcctgg taccaggggg attccacgct ttcagcgttt aataaaaccc tggttctttc
       61 cggtaatcag tccggactga ctgccgatcg catgttgacg ctctccagag ccggacaggc
      121 cgcagggctg acgtttaacc aggcgagtga gtcactggca gccctggtga atgccggtgt
      181 gcgtggtggt gaacagtttg atgccatcaa ccagagtgtc gcgcgttttg cttctgcatc
      241 cggtgtggag gtggacaagg ttgcagaggc tttcggaaaa ctgaccaccg accctacgtc
      301 ggggctgatt gcgatggcgc gccagttccg taacgtgacg gcagagcaga ttgcgtatgt
      361 tgcgcagctg cagcgttccg gtgatgaggc cggggcctta caggcggcga acgatatcgc
      421 cacgaaaggc tttgatgagc agacccgccg cctgaaagag aacatgggca cgctggagac
      481 ctgggcagac aggactgcac gggc
//
LOCUS       DAZKGS010000612          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZKGS010000612 DAZKGS010000000
VERSION     DAZKGS010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..262
                     /locus_tag="ACHOQY_005570"
     CDS             50..262
                     /locus_tag="ACHOQY_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681412.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
     gene            295..501
                     /locus_tag="ACHOQY_005571"
                     /pseudo
     CDS             295..501
                     /locus_tag="ACHOQY_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
BASE COUNT          175 a           82 c          110 g          134 t
ORIGIN      
        1 taaatttagc gctgtaaata aaatctaatc ctgaactgga ggtatattta tgttaaatac
       61 acagaaagcc attaatgcgg aaaaatataa cgagtgggca agaaaattct ctgagcagat
      121 ttttaaaatt actggcgatg agaatgcggc aaaaaatgaa ttagaaccgt ggacgcctga
      181 aggagccgac ccaaattatt gctggaggga ggttgatcca gttgatgctg caaatgaagc
      241 tatgagttat cacaacgatt aatgtcagga ggccgcccga aagggcggta atgaatggtc
      301 acattattta gaaaaaatat ccgcgaaaga gtagaacaac agaatttctg tttctcattc
      361 tgtttatcgt gttgatgata ccgatatccc cgttaatcct agtctggata atcggaaaaa
      421 taattgagcc agttattgaa ttgtataacg acgtggtatg ggcgtcattc aacacactgc
      481 acaataaaat taatccgtat a
//
LOCUS       DAZKGS010000613          498 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZKGS010000613 DAZKGS010000000
VERSION     DAZKGS010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..374
                     /locus_tag="ACHOQY_005572"
     CDS             30..374
                     /locus_tag="ACHOQY_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681413.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT          137 a          116 c          120 g          125 t
ORIGIN      
        1 cggcgggttt tgttttatgg agacaagaaa tgtcagattt ggctatgaag gttttgaaat
       61 ggcaatcgac tggcgatgtt ggcatcagta gcgcaactct tgcctcaatc gcatgtggac
      121 tgaaaaagaa tatctatggt catcacttcg gcgctccaca tgacgcagca gacttccggc
      181 gatgcgttgc acttgttgag cagattccag aaatcagaga ttcattcgac aaggttgcaa
      241 agcgcgttcc ggcattcaaa ggcatcctca acgaatggga ttccctcgtc gctctgttga
      301 agtctgaaat gaagatacac ggaaacaaag caccagagac ttacagaaga attagcgagc
      361 tacgcaagga ctaaccacag cctcacactc aatgaggcct gttcatttct caagatatcc
      421 agacctacca ttgccgcatc aatgcggctt ttcttgcgtg taattgcgga gactttgcga
      481 tgtacttgac acttcagg
//
LOCUS       DAZKGS010000614          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZKGS010000614 DAZKGS010000000
VERSION     DAZKGS010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..256)
                     /locus_tag="ACHOQY_005573"
     CDS             complement(89..256)
                     /locus_tag="ACHOQY_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681414.1"
                     /translation="MYNCGESYSEYDISLSDDDYYRERPCPGEVFGYDNTEDNDEAAS
                     DYALKINENSD"
BASE COUNT          158 a          105 c           76 g          155 t
ORIGIN      
        1 cgcccagatt aaaccaagta ttgagcgcct gattatgttt attcattaat atctgtcatt
       61 gattgtctta ttatcagcta cccccgtatt aatcagagtt ttcatttatt tttaatgcat
      121 aatcagatgc cgcctcatca ttatcctcag tattatcata gccgaaaact tctcccggac
      181 acgggcgttc acgataatag tcatcgtcag ataatgatat gtcgtattct gaatatgatt
      241 caccacaatt atacatgaaa tcatctcctt tggggggggc aataattagt gagtttattc
      301 gaaaaaatga tgagcgccag accaatccac ccaacttttg attataatca tgaagatact
      361 tgtctcagta caaattgaat caaactcaat gcagcatgat tcacctttat attctctcag
      421 ggaaaccaaa taccactact caactattaa gaacaaaatt cgggatgtat gattatcaga
      481 agatacctgc atcc
//
LOCUS       DAZKGS010000615          493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZKGS010000615 DAZKGS010000000
VERSION     DAZKGS010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.618"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..471
                     /locus_tag="ACHOQY_005574"
     CDS             <1..471
                     /locus_tag="ACHOQY_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFP9681415.1"
                     /translation="LTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
                     ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTL
                     KNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          139 a          114 c          127 g          113 t
ORIGIN      
        1 ctgacgtatg ttgccacaca ggagggatgg ttgtacctcg ctggcatcaa agatgtttat
       61 acgtgcgaaa ttgtcggcta cgccatggga gagcgcatga caaaagagct gacaggtaaa
      121 gctctgttta tggcgctcag gagccagcgc ccacctgccg ggctaatcca ccactctgat
      181 cgaggttcac agtactgcgc atacgattac cgggtcatac aggagcagtt tggtctgaaa
      241 acatcaatgt cgcgtaaagg taactgttac gacaacgctc cgatggaaag cttctgggga
      301 acgctgaaaa atgagagcct gagccactat cgttttaata accgggatga agccatctca
      361 gtaatacggg aatacattga gattttctac aatcgtcagc gtcgtcactc tcgtctgggg
      421 aatatctccc cggcagcctt cagggaaaaa tatcatcaga tggctgctta aaaaaagaac
      481 aaatggtagt gtc
//
LOCUS       DAZKGS010000616          491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZKGS010000616 DAZKGS010000000
VERSION     DAZKGS010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..433)
                     /locus_tag="ACHOQY_005575"
     CDS             complement(<1..433)
                     /locus_tag="ACHOQY_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP9681416.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICS"
BASE COUNT          140 a           91 c           79 g          181 t
ORIGIN      
        1 cactacaaat attaactgaa tttatatcaa tgattttttt attcttatct tttattttat
       61 ttactgtttc aatgcaatca gcggcaagtt ggagtggatt tagatttttc agatatggtc
      121 ttatgatctt tcccttactt aataaatagc atgtatagga ttcggaaagt ccagcatcta
      181 ttttgtacct cattatatta gataaggatg tgagaaattc cttgttatca ttcgcagcca
      241 tatatgccag tccgagttca gcctctgcgg ccttacaggc aaaatagctt agtgaacacg
      301 cttttcgtcc ctctttagta ctggctgata ttagtgccca taatattgct ttgtctgcca
      361 aatctaactc tctctgaaat tgtcttgcag taactgcact cgcagaaata gagaaaaaaa
      421 tacaattgtt aataaccttg ccatattttt tagtccgtaa ctgttaatgt gctatctggc
      481 tctgaaaata t
//
LOCUS       DAZKGS010000617          486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZKGS010000617 DAZKGS010000000
VERSION     DAZKGS010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..358)
                     /locus_tag="ACHOQY_005576"
                     /pseudo
     CDS             complement(1..358)
                     /locus_tag="ACHOQY_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          148 a          123 c           86 g          129 t
ORIGIN      
        1 ggacactgaa tagactctgg cgggcactgg aaatttcctg aactaacagg aaacttacat
       61 tgagatattt tgtcctgaat actctcttgc gattgtgctt cacctgagtc cgaaagcata
      121 ctgagcatcc tatcgcgaag agcttcaggg ccgttattaa acccataaag gaataactca
      181 gtaagcaatc ccccactact gccgctggat agaaaacgcc catcggtttc attaaataca
      241 atgctgacag tctcattacc taatgtaaac tcccctcgac cgctagatgc tacttgagct
      301 cgaaggacat tcaatccatt aaacatgagc ttgatctaat ctccgaattt aacggcatta
      361 taaaactccc tattttaatt taaactccag acttaaatag ctttaacaaa catctgcctt
      421 acatgacaac aaaaaccgga gccggactcc ggttttgtga agctgtcggg ttacttcatc
      481 ccgcca
//
LOCUS       DAZKGS010000618          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZKGS010000618 DAZKGS010000000
VERSION     DAZKGS010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>485
                     /locus_tag="ACHOQY_005577"
     rRNA            <1..>485
                     /locus_tag="ACHOQY_005577"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZKGS010000619          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZKGS010000619 DAZKGS010000000
VERSION     DAZKGS010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..264)
                     /gene="gpU"
                     /locus_tag="ACHOQY_005578"
     CDS             complement(<1..264)
                     /gene="gpU"
                     /locus_tag="ACHOQY_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9681417.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPV"
     gene            complement(261..>485)
                     /locus_tag="ACHOQY_005579"
     CDS             complement(261..>485)
                     /locus_tag="ACHOQY_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681418.1"
                     /translation="RIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTTAF
                     KQNIERIRRERLPKELGYALQHQLRMVIKR"
BASE COUNT          112 a          149 c          114 g          110 t
ORIGIN      
        1 caccggataa atccgggact ccatccacgc atccagctct gaatccggca cctgagcagg
       61 caggaaaact tcaatatgca gctccgcctg ccaggtatcg ctgtccagct cttcgcccgt
      121 gtattcagcg ccggtgagat aaacggcaac tgccggaaaa tccgcctcat caaaaacagc
      181 ggggcgacca tcaaaaagcg tcgccccggt gtcatgcttc tccagtgcat ccagtacggc
      241 tgcacggagt tcagtatgtt tcatcgcttt attaccattc tcagttgatg ctgcagcgca
      301 tagcccagct ctttcggaag acgttcacgc cgtatccgtt caatattctg tttaaacgcc
      361 gtggtaagcg gcaccgccat cgggattttc accacatcaa tggggtaacg gtttttcccg
      421 gccacacgct gcatgacatg ccaccgccca tttttcagtt gctgaataaa cgcgccggga
      481 atacg
//
LOCUS       DAZKGS010000620          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZKGS010000620 DAZKGS010000000
VERSION     DAZKGS010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            280..399
                     /locus_tag="ACHOQY_005580"
                     /pseudo
     CDS             280..399
                     /locus_tag="ACHOQY_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          131 a          122 c           74 g          155 t
ORIGIN      
        1 tatctcaatt tttaaacata aaatgcgaat tatttagtac aaaaagtgaa cttttgatga
       61 tctttcccta atctcacata ggatcagatc tcgtctgatc gttccccctg cgctcactac
      121 catggctctt taccacagca gccggggttg tttgggaccg acttccgaca gcagatcccg
      181 gtgggcctgc caccatctct tcacaagctt atgcttatta catgcaaaac gtcccgtttt
      241 gcatctttgt gcctacggca tccctgtgat ctttccctga atctgcccct gagaacctga
      301 atcaaacgtc tgactcgcag aaccatctgt ttctcgcgct ctgtagaact tcatgaaaaa
      361 gtcatcggtg cttttatcga aaaatacatg ttctactaat tgaagtcatc atcaaaaaat
      421 ttaattaatc gaatcatacc tataaccttt taattttact tttttgtctt caacattaaa
      481 ca
//
LOCUS       DAZKGS010000621          479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZKGS010000621 DAZKGS010000000
VERSION     DAZKGS010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..176)
                     /locus_tag="ACHOQY_005581"
     CDS             complement(<1..176)
                     /locus_tag="ACHOQY_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9681419.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYE"
     gene            complement(244..>479)
                     /locus_tag="ACHOQY_005582"
     CDS             complement(244..>479)
                     /locus_tag="ACHOQY_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFP9681420.1"
                     /translation="SSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFS
                     VSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          123 a          127 c          107 g          122 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatgccc ttcaggtcat cgctgcctgg catatcagta
       61 tgggtctgaa gatacccggc gcttagtgtg gactgatgct ctgctgcgct cgctggcgta
      121 ccagcggcaa ccagccagac tactgcggac agaataacag cacataattt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa
      241 aattcagctg atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct
      301 gatacttgct gtgggatacc agtcatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagt
//
LOCUS       DAZKGS010000622          476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZKGS010000622 DAZKGS010000000
VERSION     DAZKGS010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>476
                     /locus_tag="ACHOQY_005583"
     rRNA            <1..>476
                     /locus_tag="ACHOQY_005583"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZKGS010000623          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZKGS010000623 DAZKGS010000000
VERSION     DAZKGS010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>474)
                     /locus_tag="ACHOQY_005584"
     CDS             complement(<1..>474)
                     /locus_tag="ACHOQY_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFP9681421.1"
                     /translation="VHDEWLGDLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKISG
                     VELSDINKEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQL
                     SFYWQGGQCKPEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLPKSGEIKPT"
BASE COUNT          145 a          100 c           66 g          163 t
ORIGIN      
        1 gttggtttta tttctccact tttcggtaaa agatatttga ctatagtcca taagggatta
       61 tcagaaaata gccattcaag atttcctgca ccgtatttta tcagttcaga acaaaaccgt
      121 tcttcgccat attctggttt acattgccct ccctgccaat agaatgataa ctgcttcaca
      181 ttttgatatc ctatctctct gatcagagtt tctcgatata atcgtgaaaa aatatagtca
      241 gggcattgaa tttcaaataa tgcactactt agagcaagca atcttatttt cccaattgac
      301 tgatataaaa aaccttcctt atttatatca gataattcca ctccagaaat tttatcttgt
      361 atttcctgac gcaatatctt gttgagttga ttacgagtag aactactttc tcttaaatgc
      421 cgaaaactaa cctgattgac accggttaaa tcacctagcc attcatcatg gacc
//
LOCUS       DAZKGS010000624          474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZKGS010000624 DAZKGS010000000
VERSION     DAZKGS010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>474)
                     /locus_tag="ACHOQY_005585"
     CDS             complement(<1..>474)
                     /locus_tag="ACHOQY_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP9681422.1"
                     /translation="ATLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEYTF
                     LDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASSAGA
                     AAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTLDVEEEE"
BASE COUNT           92 a          147 c          126 g          109 t
ORIGIN      
        1 tgctcttcct cctccacatc cagcgtgcgc ccttcctctg cggctttggt catgacctcc
       61 tcaagggagg ctgccagcgc tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata
      121 ttcattatgg tcttttcctt atgaaaaacg gttgttgact gtgccgcagc gccggcagaa
      181 gatgcgattt tcaccaccgg tttccggttg ccggacgcgg cagaaaacgg gcggtcgtaa
      241 gatttaatgg tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc
      301 atcagttccc agcgcagaaa atgcagtccg cctccgtcca gaaaggtgta ttcatgggga
      361 cggaagccca cggacagccc cctgaccagc ccggtcttaa tggccgccca gacctcatcc
      421 agccgggcag ccagttgcga cggcatatcc ggtacgggct tcaccagtgt tgcc
//
LOCUS       DAZKGS010000625          470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZKGS010000625 DAZKGS010000000
VERSION     DAZKGS010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>470)
                     /locus_tag="ACHOQY_005586"
     CDS             complement(<1..>470)
                     /locus_tag="ACHOQY_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681423.1"
                     /translation="DDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDT
                     TKIKHARSVIDHIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNL
                     SNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLIK"
BASE COUNT          162 a           85 c           63 g          160 t
ORIGIN      
        1 cttaattaat tgatagaagt accacttcgc aaaatcaggt tgcttcatga aaaaatcctt
       61 caaaaaaata tcatttacta aaatgctgac aggcacttca ttaaatagca acttaacatc
      121 atcaaataac cttagtatta agcgtgattt ttcttgattg cttaagttaa aatcattgac
      181 atatacaacc aacttctccc aaatcaattc ctgtaactca gggctgtttt tatagggttc
      241 tttattgagg ttgaaaaata atgtctcagc tattttagac tgatcgcagt taaaatcaat
      301 atgatcgatc acggagcggg catgctttat ctttgtagta tcatcagaat attgcatact
      361 ttgattaaaa caccactcca gaatttttac atcattaata tatggcaatt ttaaaaaagt
      421 cttcaacatc gaatcgatga ccttttcgag attaactttt gcatcatcta
//
LOCUS       DAZKGS010000626          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZKGS010000626 DAZKGS010000000
VERSION     DAZKGS010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..268
                     /gene="yhhH"
                     /locus_tag="ACHOQY_005587"
     CDS             <1..268
                     /gene="yhhH"
                     /locus_tag="ACHOQY_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFP9681424.1"
                     /translation="MRKYESEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKP
                     YEITELTTSWVVEGTIHSDQIAGGVFIIEIGKNDGRILNFGHGK"
     gene            373..>468
                     /locus_tag="ACHOQY_005588"
     CDS             373..>468
                     /locus_tag="ACHOQY_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFP9681425.1"
                     /translation="MSNTYQKRKASKEYGLYNKCKKLNDDELFRLL"
BASE COUNT          180 a           58 c          109 g          121 t
ORIGIN      
        1 aatgcgcaag tatgaaagtg aaggtaaata taccgttaga aatttggtta aaaataaagc
       61 tatagcattg gaattagctg agatatatgt taagaatcgt tatggacagg atgccgcaga
      121 agaagaaaaa ccatacgaaa tcactgagtt aacaacaagt tgggttgttg aaggtaccat
      181 tcactcagac caaattgctg gtggggtttt tattatagaa ataggcaaaa atgatgggag
      241 aattctgaat tttggccacg gaaaataagt atgtaaggat aatcaaacat cgtgtgcgct
      301 gatggcagag taggtggagg actccagaca gtcaaacgat agaaaaagat agcctttatg
      361 gaggttcctg caatgtcaaa tacataccag aaaagaaagg caagtaaaga gtatggttta
      421 tataataaat gtaagaaact aaatgatgat gaattatttc gcttactt
//
LOCUS       DAZKGS010000627          466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZKGS010000627 DAZKGS010000000
VERSION     DAZKGS010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(165..374)
                     /gene="fumD"
                     /locus_tag="ACHOQY_005589"
     CDS             complement(165..374)
                     /gene="fumD"
                     /locus_tag="ACHOQY_005589"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9681426.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          144 a          107 c           70 g          145 t
ORIGIN      
        1 ttatttgtga cgttcattgc agaaaataaa aataatatta tcgttataaa ttaaaataca
       61 taccgcgatt cgtataattt cagcagttgt tatttagaat gattaactgg accgcaactg
      121 aagcaagaat atcaggatga agcttaccgc ctcatcctga atggtcattt taccagtgcg
      181 ttaatgacgg tttccatcgc ctgcttttcc agctcactgc gctgaatgcg tttattggct
      241 aatgctgtac gtaatacgcc tgcgataaca atatgctttg gttcctgccc cagatcgcgc
      301 atttccagca caactttacc gacaactctg cacatttctc ggtacagttc atcctctttt
      361 gtccgatttc ccattcttta tacctattta tcatgccaac tatcagcata tatcaatcta
      421 accaattaaa caaaaatcaa acaaaatcag acaaataacg cgataa
//
LOCUS       DAZKGS010000628          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZKGS010000628 DAZKGS010000000
VERSION     DAZKGS010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>460
                     /locus_tag="ACHOQY_005590"
     CDS             <1..>460
                     /locus_tag="ACHOQY_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9681427.1"
                     /translation="HVLKSAVGAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRF
                     GQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVL
                     TEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVSPASITHD"
BASE COUNT           92 a          121 c          144 g          103 t
ORIGIN      
        1 catgtcctga aatcggcagt gggcgcgggg accaccacgg atccgcagtg ggcaggcagc
       61 ctgtctgaat atcaggaata cgcacaggac tttattgatt acctgcgtcc gcagaccatt
      121 atcgggcgat ttggtcaggg cgggatccct gcacttcgtc aggtgccatt caatatccgt
      181 gtgcacgccc aggtgtccgg cggtgctgcc ggctgggtgg gtgagggtaa ggcaaaaccc
      241 ctgacgaagt ttgattttga atccatcacc ttcagtcatg cgaaggtgtc ggccattgcg
      301 gtactgacgg aagaattgat ccgtttttcc agtccggctg ctgatgcact ggtccgtaat
      361 gcgctggcgg aagcggtggt ggcgcgtctg gatacagact ttgtggaccc gaaaaaagcc
      421 gcagtggcag atgtctcccc ggcgtccatc acccatgatg
//
LOCUS       DAZKGS010000629          457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZKGS010000629 DAZKGS010000000
VERSION     DAZKGS010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>457
                     /locus_tag="ACHOQY_005591"
     CDS             <1..>457
                     /locus_tag="ACHOQY_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9681428.1"
                     /translation="GHAWAHETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAE
                     YVRRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKL
                     AEGVLVHGDQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNA"
BASE COUNT           95 a          107 c          165 g           90 t
ORIGIN      
        1 ggccatgcct gggcgcatga aaccgcggtg gtccgacgga agagcgaggc gtcccggttt
       61 caggatcttg ttgcctgtgg agatatgacc attgtccggc gtgtcgggga tgacacggcg
      121 gaagtggcgg aatatgtgcg tcgcattcat gaggctgagt tactggacca tatcggtatt
      181 gacccgtcag gggtggggca gattctggat tcactggcgg aagccgggat ccccgacgga
      241 attgtggtgg ggataagcca gggctggaaa ctgggcgggg ccattaaaac caccgagcgc
      301 aaactggctg aaggggtgct ggtgcatggt gaccagcccc tgatggcctg gtgtgtcggc
      361 aatgcccggg tggagcctaa aggtaacgcc attcttatca ccaaacaggc cagtggacgg
      421 ggaaaaattg acccgctgat ggcgctgttc aatgcgg
//
LOCUS       DAZKGS010000630          457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZKGS010000630 DAZKGS010000000
VERSION     DAZKGS010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..203)
                     /locus_tag="ACHOQY_005592"
     CDS             complement(<1..203)
                     /locus_tag="ACHOQY_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFP9681429.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWT"
     gene            complement(211..>457)
                     /locus_tag="ACHOQY_005593"
     CDS             complement(211..>457)
                     /locus_tag="ACHOQY_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9681430.1"
                     /translation="EALDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPAMSAIPA
                     LSGLITTMVTQGYEYRRDDDMALWSSADLTYSITYEM"
BASE COUNT          102 a          138 c          106 g          111 t
ORIGIN      
        1 gtccagtccg cgtcttcatc atccaggtag ttatcatcgt agggttctgc cgtcatctcg
       61 cccggcgtca gatcctttac ctgtgccagt cgctgccagt tatcgtctga caacgggttt
      121 gcataggcat caccctgacc gttataaacc cacagggtgg taccggcacc ttttaccggt
      181 gccagaggat ttggtgttgg catatcgtcc tcacatctcg tatgtaatgg aataagtcaa
      241 atcagcagaa ctccataacg ccatatcgtc atcacgacga tactcatagc cctgcgtaac
      301 catcgtggta atcaggcctg acagtgccgg gatcgcactc atcgccggat aaatccggct
      361 ttccatccac gaatccagct ctgaatccgg cacctgagca ggcaggaaaa cttcaatatg
      421 cagctccgcc tgccaggtat cgctgtccag cgcttcg
//
LOCUS       DAZKGS010000631          456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZKGS010000631 DAZKGS010000000
VERSION     DAZKGS010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(57..>456)
                     /locus_tag="ACHOQY_005594"
     CDS             complement(57..>456)
                     /locus_tag="ACHOQY_005594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681431.1"
                     /translation="YIPSLMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYL
                     LNHYTESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQN
                     PFNIIIVICTKEHIERLELMYQGKSDGFFT"
BASE COUNT          131 a           91 c           91 g          143 t
ORIGIN      
        1 aaacttttaa ttatatttca tagcacacaa aagtcaacag aagccctctc gtgctcctac
       61 gtaaaaaatc catcactttt cccctgatac atcaactcaa ggcgttctat gtgttcctta
      121 gtgcatatta cgattattat gttaaatgga ttttggccta tattttgacc tgcacccgca
      181 taaacactgg tttcatcgct tacagccctt gtgagacgtg gtgctgtttc tagcatctga
      241 tccgacataa aaccgatcat tctcgtgttt agtgaaagtt tagtgagaga cattacagaa
      301 aggctttctg tgtaatgatt taataggtaa aaacgatgtt gattacgcaa gaaaattcct
      361 tgctgatatt tggggattct ggattttcca gatcgggggg cgctgaaaat cgcgttaagc
      421 acttctcgca atctagccat aagggaaggg atatag
//
LOCUS       DAZKGS010000632          454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZKGS010000632 DAZKGS010000000
VERSION     DAZKGS010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..454
                     /locus_tag="ACHOQY_005595"
                     /pseudo
     CDS             1..454
                     /locus_tag="ACHOQY_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSfl10 family transposase"
BASE COUNT          140 a          107 c          116 g           91 t
ORIGIN      
        1 cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc aacagcgtat
       61 tttgcacagg agtcgctgaa aaatacgcgt taatcgaaca atggcgacaa caatttccca
      121 ttgaagcgat gtgtcaggta tttggtgtat ccaggagcgg ttattacaac tgggtacagc
      181 atgaaccctc agacagaaaa caaagtgatg agcggctaaa actggagatt aaggtggcac
      241 atatccgcac tcgcgaaaca tatggaaccc ggcggctcca gacggagctg gcagagaatg
      301 gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa ggagctaagg ctacgctgta
      361 agcagaaacg caagttcaga gcgactacga actcgaacca caatctgcca gttgcgccaa
      421 atctgctgaa ccagacgttc gctcctacag cacc
//
LOCUS       DAZKGS010000633          453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZKGS010000633 DAZKGS010000000
VERSION     DAZKGS010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(109..339)
                     /gene="ynfN"
                     /locus_tag="ACHOQY_005596"
     CDS             complement(109..339)
                     /gene="ynfN"
                     /locus_tag="ACHOQY_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFP9681432.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(344..409)
                     /gene="ynfR"
                     /locus_tag="ACHOQY_005597"
     CDS             complement(344..409)
                     /gene="ynfR"
                     /locus_tag="ACHOQY_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFP9681433.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
BASE COUNT          148 a           93 c           81 g          131 t
ORIGIN      
        1 cgtgtaaaag ataagatgaa acatcgcaat aatcaacata cgatagtcta aattttacac
       61 aaacagacaa agagaatttt cctgaattat caatacaata gcatcaaatc aactcaagag
      121 ccttattgct gcttccagaa tttcttctga agtaacatgt cgatccgcgg ctacataaat
      181 gactttatga tctccggtca gagatggaaa ccctgcggcc attacagtaa ggtgtgtttt
      241 ttcgccattt ggatattcac gcatgatggt gttaactcca gtcatcgctg gcactaccac
      301 tgctggttca gagttaaaaa aaactatgat ttttttcatg atgttaccgt agtatgtgag
      361 tatccatcga atagatacca agcaaaaaag ctcccgaagg agccttcatt ttcacttttt
      421 taaatccaac gacagacggc tggcatttaa gta
//
LOCUS       DAZKGS010000634          453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZKGS010000634 DAZKGS010000000
VERSION     DAZKGS010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHOQY_005598"
     CDS             <1..>453
                     /locus_tag="ACHOQY_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9681434.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          101 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZKGS010000635          453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZKGS010000635 DAZKGS010000000
VERSION     DAZKGS010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHOQY_005599"
     CDS             <1..>453
                     /locus_tag="ACHOQY_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9681435.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gctgcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZKGS010000636          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZKGS010000636 DAZKGS010000000
VERSION     DAZKGS010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..282)
                     /gene="tccP2"
                     /locus_tag="ACHOQY_005600"
     CDS             complement(<1..282)
                     /gene="tccP2"
                     /locus_tag="ACHOQY_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFP9681436.1"
                     /translation="MINSINSFFSSLPRSISSAIRSSTFTVSQHKSTPNTVKTSSPFS
                     PSNSPASATTIFKVKNSYTESGLQRPTSYTQSSIEKNALHRPLPDVAQRL"
BASE COUNT          129 a           63 c          102 g          155 t
ORIGIN      
        1 cagacgctga gccacatcag gtagaggccg atgtaaagcg tttttttcta tacttgactg
       61 agtataagat gtgggacgtt gaagtcctga ttctgtataa gaatttttta ccttaaaaat
      121 agttgttgca ctggcaggac tgttgctggg agaaaaaggt gagctggttt ttaccgtgtt
      181 aggtgtgctt ttgtgctgcg agacagtgaa ggtactgctg cgtatagcac ttgatatact
      241 gcgaggaagg ctggaaaaaa aagaattaat gctatttatc atatagataa ccttataaga
      301 gctttattgg atagtcttat ttatccgttt acatgaaatc taccttttgt gaaattctcg
      361 tcaatatttt actcaaaatg atcggctgta ggaataaact atttgatatt attacgtttt
      421 tagggttgtt ttcaatcagc tatagatag
//
LOCUS       DAZKGS010000637          447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZKGS010000637 DAZKGS010000000
VERSION     DAZKGS010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..344
                     /locus_tag="ACHOQY_005601"
     CDS             <1..344
                     /locus_tag="ACHOQY_005601"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9681437.1"
                     /translation="GVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALIISFWLD
                     VDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAVLITILV
                     AIIIWGVLLLS"
BASE COUNT          125 a           75 c           99 g          148 t
ORIGIN      
        1 ctggtgttac cagaattatt aaagcagcgg gttattcatg gaaaggctta tgtgcggcat
       61 ggcgctatga agcagccttt cgtcaggagt gcattctggt tctgtcagca ctgataatat
      121 ccttttggct ggatgttgac tctgttactc gagtattact cattggttcg gcaatgatgg
      181 taattattgt tgaaataatt aacagtgcaa ttgaagcagt tgttgacaga attggttcag
      241 aacatcacga attatcaggc agagcaaaag atatgggttc agccgcagtg ttgattacga
      301 ttttagttgc tatcattata tggggagttt tattactttc ataacgccca acatctaaat
      361 tattcacagt tacctggtta atatttactt aaggataaga ggtattcacc tcttatcctc
      421 agaaaaatgt cagtatctat ctgcagg
//
LOCUS       DAZKGS010000638          444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZKGS010000638 DAZKGS010000000
VERSION     DAZKGS010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>444
                     /locus_tag="ACHOQY_005602"
     CDS             <1..>444
                     /locus_tag="ACHOQY_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9681438.1"
                     /translation="NLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYI
                     TQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKE
                     YGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTH"
BASE COUNT          144 a           82 c          111 g          107 t
ORIGIN      
        1 aacctgctga atgaagagaa cccgcatcag ctgcagcagc ttatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      121 gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaacct gtatacatat
      181 ccgctgagcc cggtgaatag catggatcca ttaggattat atgaatttaa atcaaaaaat
      241 atagatgata ttggaatatt tgcattggca atgtgtaatg gagaatcaat taacgagaat
      301 aaagaatatg gtggactaat atgtaagaag caaggtgaat atttccccat gaatccgata
      361 agttcaaatg ataatgatag tgtagacttg cgaaatataa aatgccctga aggttcagag
      421 agagtaggcg attatcacac tcac
//
LOCUS       DAZKGS010000639          443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZKGS010000639 DAZKGS010000000
VERSION     DAZKGS010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..>443
                     /locus_tag="ACHOQY_005603"
     CDS             44..>443
                     /locus_tag="ACHOQY_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFP9681439.1"
                     /translation="MLPTTNISVNSGVIYFESPVGSPSNEDVKVALEKWCAEGEFSEN
                     RHEVASKILNVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSIVGCSSLEVINCSIPDNVSNLSLCHCSS"
BASE COUNT          140 a           73 c           90 g          140 t
ORIGIN      
        1 attggtgtta ttttcagtgg actaagttga aggagtgcga taaatgctgc ccacgacaaa
       61 tatctctgta aattctggag taatatattt tgaaagtcct gtaggttcac catctaacga
      121 ggatgttaaa gttgcactcg aaaagtggtg cgctgaggga gaattcagcg aaaatcgtca
      181 tgaggttgca tcaaaaatac ttaatgttat aagtactaat ggagagactt tatcaatcag
      241 tgagccaata acaacgttac cagacttgct tccaggttct ctgaaagaac tggtattgaa
      301 tggatgtaca gagcttaaat caataaactg tttacccccc aacttatctt cattaagtat
      361 agttggatgt tcatcattag aggttataaa ttgcagcata cctgacaatg tcagtaattt
      421 atctttatgc cattgtagtt ctt
//
LOCUS       DAZKGS010000640          438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZKGS010000640 DAZKGS010000000
VERSION     DAZKGS010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..227)
                     /gene="tnpB"
                     /locus_tag="ACHOQY_005604"
     CDS             complement(<1..227)
                     /gene="tnpB"
                     /locus_tag="ACHOQY_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9681440.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGR"
     gene            complement(224..>438)
                     /locus_tag="ACHOQY_005605"
     CDS             complement(224..>438)
                     /locus_tag="ACHOQY_005605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681441.1"
                     /translation="TENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLNISCEVT
                     FRHGSLRLNGAISENILNLLIRELKR"
BASE COUNT           93 a          114 c          124 g          107 t
ORIGIN      
        1 aagcggccgc gctccagccg tttggtcagc agacacagtc catcgccggt agaccagagg
       61 agctttacct gactgccatt acgcccacgg aagataaaaa cgtgacctga catcggatcg
      121 tctttcagcg tcgtctgcac ctttgccgcc aggccgttga agccgtttct catatcggtg
      181 atgccagcga ccagccagat ctttgtccct gatggtaatg ggatcaacgt ttgagctccc
      241 gtatcagcag gttcaggata ttttcgctga tggcaccatt cagacggagt gatccgtgcc
      301 ggaacgtcac ttcacagctg atattgagtg attcaggtgt cgcagcagct acaggttgtg
      361 accgggggga ggttgtaggg acaacatctt cggcatcaag tgttatcggg agcagctcag
      421 gcacgttgtt ttctgttg
//
LOCUS       DAZKGS010000641          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZKGS010000641 DAZKGS010000000
VERSION     DAZKGS010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..342)
                     /locus_tag="ACHOQY_005606"
                     /pseudo
     CDS             complement(1..342)
                     /locus_tag="ACHOQY_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT           82 a          126 c          115 g          114 t
ORIGIN      
        1 gatctttccc tggctccacg tctggtcgtt atcggaccag cggatgatcc ggtccgcagc
       61 gtcgtactca taaaacaggt ggtagtccag ccgcgcatcc gcttcggtca gccgcccccg
      121 cgcatcctgt tcgtaggtcg ccagcgtctg tatgccgtta tccgtgcggt gaatggcttt
      181 caggtagccg ctggcatggt acgtcagttt aagccggatc ccgtcggtgt gactgacaca
      241 cgtgatccgg tactggctgt cgcgctcaaa cgtgatgcgg ttgctggcgg tgtcactcat
      301 cccgcccagc atatacatcc ggtcgtcggg gttgtgtgcc aggtgtcata tcaagttggc
      361 cttacggaat ttcaactgcc tccttgccat taatcaatat atcaattgat gaagataaat
      421 tatccccata cttccaa
//
LOCUS       DAZKGS010000642          428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZKGS010000642 DAZKGS010000000
VERSION     DAZKGS010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..271
                     /locus_tag="ACHOQY_005607"
     CDS             47..271
                     /locus_tag="ACHOQY_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681442.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
BASE COUNT          114 a           94 c          110 g          110 t
ORIGIN      
        1 tccgcactca gtgtactaag caggcttttt attatccgga ggatgtatga agaaattaca
       61 ggtaacggta aaaccttttc agggaacaat tccgttccgt gttttgcaac atggccgtgt
      121 tctgcttgaa gaggtgttca gaggtaaatg cactgaatgt tattcacgaa catatgaagt
      181 gaatgccacg catgaagaat tcaccgttga gtgtgtgatg aatactgata aatgccgaat
      241 ggtatccgct gaattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt
      301 cgccgtattc ctgcattaac agagaccgca gcccgacagg gagactcctc tgcgagagtg
      361 tgcggggata atcaaaaacg atacacaccg gggtttaccg cgtaaacgga gcgcggcgtt
      421 ctcccctc
//
LOCUS       DAZKGS010000643          428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZKGS010000643 DAZKGS010000000
VERSION     DAZKGS010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>428
                     /locus_tag="ACHOQY_005608"
     CDS             <1..>428
                     /locus_tag="ACHOQY_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681443.1"
                     /translation="STLKGALDINSLIIDYDNEDVQSYWEDVVLPNLNDNGNKALTRD
                     EIIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIAEYAVSRNAIISSNTSGNVI
                     RGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLNDS"
BASE COUNT          154 a           61 c           90 g          123 t
ORIGIN      
        1 tcgactctca aaggtgctct ggatatcaac agcttgatca ttgactacga taatgaagat
       61 gtccaaagtt attgggaaga cgttgtatta ccgaacttaa atgataatgg taataaagct
      121 ctcacaagag atgaaataat agcctatatt aaagacagta agccttgtag aagaattaaa
      181 agattaatac atgatgatgc tgtgttggag gatcaaaagg ataaaaactt cattgcaaaa
      241 atgattgcag aatatgctgt gtctagaaat gcaattattt ctagcaacac atctggcaat
      301 gtcattcggg gtgatgaaag cggagtaaaa tggattcaat gtggtaatat ctttgtctcc
      361 ctatttcata aggttcaaga tgatcatgaa aacgatggag ataggatttg gcaaactctc
      421 aatgattc
//
LOCUS       DAZKGS010000644          425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZKGS010000644 DAZKGS010000000
VERSION     DAZKGS010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..381
                     /locus_tag="ACHOQY_005609"
                     /pseudo
     CDS             28..381
                     /locus_tag="ACHOQY_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310463.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          143 a           60 c           83 g          139 t
ORIGIN      
        1 aattttgtta acaatatgag gtgggagatg ttttctacaa acttaataca gagtaattat
       61 ggtgatttaa atattaaaac tctggctttt gactctttta aagaaaggct tgagtctacc
      121 atggcggcac tcactttctt tatatcaacg ggtcaatgtg actgcgatga aatagcagaa
      181 tcaaacttca atcgtatgat tgcctatatg tctaacatta actatgatgc ctcaaagcca
      241 ggagcgccag cacttagttt tgatacatat ctgcaggaca atgttaaata tagagtaata
      301 attaataatc tttatggcgg tgagtttcgg ataagagata ttaatgagaa ttttgttggg
      361 atggatttta cctgtgaatt ctgacccgaa aaaatgacga atttgatcaa tataaaaaat
      421 aggtt
//
LOCUS       DAZKGS010000645          424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZKGS010000645 DAZKGS010000000
VERSION     DAZKGS010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHOQY_005610"
     CDS             <1..330
                     /locus_tag="ACHOQY_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9681444.1"
                     /translation="ATFSLRMKGKPVSFVVPLAFVKNLDKTLTVNTGALLTMSVSANG
                     GTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTV
                     TVSAAAG"
     gene            297..>424
                     /locus_tag="ACHOQY_005611"
     CDS             297..>424
                     /locus_tag="ACHOQY_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9681445.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
BASE COUNT          111 a           93 c          137 g           83 t
ORIGIN      
        1 gcaacgtttt cactgcgtat gaaaggcaaa ccggtgtcct ttgtggtacc gctggcgttt
       61 gtgaaaaatc tggataaaac acttaccgtg aatacaggtg cgctgctgac aatgtcagtc
      121 agtgccaacg ggggaacgcc gccgtataaa tacgcctgga agaaggatgg tcagccggtt
      181 gacgggcaga cgacagacac cttcagtaag ccaggtgcgc agtccgctga tgcggggaaa
      241 tatacctgcg tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc tgttgaatgc
      301 accgtgacag tgagcgcagc cgccggataa ggggatgggt catcatgaaa aaggatctga
      361 aaacgctggc gctggccaga ctgtcagggt ttcgtcataa aacggtgaag gtgccggaat
      421 gggg
//
LOCUS       DAZKGS010000646          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZKGS010000646 DAZKGS010000000
VERSION     DAZKGS010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>421
                     /locus_tag="ACHOQY_005612"
     CDS             <1..>421
                     /locus_tag="ACHOQY_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFP9681446.1"
                     /translation="QNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTKDT
                     ITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKT
                     IAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWA"
BASE COUNT          136 a           99 c          107 g           79 t
ORIGIN      
        1 tcaaaacgag ggaggttccc ctaaaatgag ggacatccct caaaacgagg gaaaatcccc
       61 taaaacgagg gataaaacat ccctcaaatt aggggattgc tatccctcaa aacaggggga
      121 cacaaaagac actattacaa aagaaaaaag aaaagattat tcgtccgaga attctggcga
      181 atcctctgac cagccagaaa acgatctttc tgtggttaaa ccggatgctg caattcagag
      241 cggcagcaag tggggaacag cagaagacct gaccgccgca gagtggatgt ttgacatggt
      301 gaagaccatc gcaccatcag ccagaaaacc gaattttgca gggtgggcta acgatatccg
      361 cctgatgcgt gaacgtgacg gacgtaacca ccgcgatatg tgtgtgcttt tccgctgggc
      421 c
//
LOCUS       DAZKGS010000647          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZKGS010000647 DAZKGS010000000
VERSION     DAZKGS010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..>420
                     /locus_tag="ACHOQY_005613"
     CDS             143..>420
                     /locus_tag="ACHOQY_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFP9681447.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
BASE COUNT          104 a          100 c          117 g           99 t
ORIGIN      
        1 ggcctttttt gtatctgcgc cacgcccggc gcatatcaac cacagagcct ttcgggggtg
       61 agcttacgga gtggtcagtg tgactttctc tgtgggcaga tcgctcccgg gcgttggctc
      121 acccacccaa aggaacgtca cgatgtttgg aatcttcaaa aagaaaaccc gcagagcggc
      181 agcggaaatt aaaaagtttg agaaacgcga tctggcacag gcggtgatta acgctgcata
      241 cctggtggcc tatgcagatg gtgaatgcga ggcatccgag aaagcgaaga tcgaacaggt
      301 cttacgtaat cagcctgcgt tgtctgcgtt tacctcggaa attaatgcga ttagcgcaac
      361 tattatcggt cagctggata ccaattttaa aattggtcgt cgtgccgcgt tacgcgagat
//
LOCUS       DAZKGS010000648          417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZKGS010000648 DAZKGS010000000
VERSION     DAZKGS010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.651"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(293..397)
                     /locus_tag="ACHOQY_005614"
     CDS             complement(293..397)
                     /locus_tag="ACHOQY_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681448.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
BASE COUNT          107 a          115 c           78 g          117 t
ORIGIN      
        1 aacagatgac gatcagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct
       61 tgcctttcgg cacgtaagag gctaacctac atttgtgaga catagattgg gcctcagatt
      121 aatgttaagc gtcttgcagg acgcgtaatg ttaactgggg cttttctcta tctgcctttt
      181 ggtgttcatg cctgagacag atagcctcaa gcacccgcag caattctact taactctcct
      241 tttcccgcaa accgttttta cccgatatgg gaattcccat atcgtaatga attcagttcc
      301 ctagtcgatc catcaaaaac acaaccaggc agtaaacgcc cacaacagca acaacagcca
      361 gcgcaccttc cattgccagt gatatatcat ccgacatatt ccctcctttg gtgttaa
//
LOCUS       DAZKGS010000649          417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZKGS010000649 DAZKGS010000000
VERSION     DAZKGS010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            294..>417
                     /locus_tag="ACHOQY_005615"
     CDS             294..>417
                     /locus_tag="ACHOQY_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFP9681449.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT          136 a           94 c           76 g          111 t
ORIGIN      
        1 ttcccgaaaa aatccattaa ataagaagcg agccgttgcg gaggcttgct ctctccttta
       61 acctcatgtg caatatccgt aacgcgctca cataaatcat catctgataa tataacaaca
      121 cccattattt tattctgacc taaaaacttc ttatgaaata aacgtttacc cgacgtatct
      181 gatcccgctg tttttacctg tatcctttaa catacttgag aagcccgctg aaaaatatac
      241 cgtaaaggcg gttctctgtc ttccataaac ctgtcggata agaagggcat taaatgaaaa
      301 ccaaaaaagt tatgatgatt gcactggtca gctctgcgct ggctctttca ggatgtggag
      361 ccatgggcac agcgatcaaa aaacgaaatc tggaagtaaa aacccagatg agtgaaa
//
LOCUS       DAZKGS010000650          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZKGS010000650 DAZKGS010000000
VERSION     DAZKGS010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>415
                     /locus_tag="ACHOQY_005616"
     CDS             <1..>415
                     /locus_tag="ACHOQY_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP9681450.1"
                     /translation="REYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQ
                     GRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAV"
BASE COUNT           98 a          120 c          137 g           60 t
ORIGIN      
        1 ccgggaatat gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt
       61 acgctaccgc tacgatgacg cgcacagtga gttaccggcg acgacaacgg atgcgacggg
      121 cagcacccgg cagatgacgt ggagccgcta cgggcagttg ctggcgttca ctgactgctc
      181 gggctaccag acccgctatg aatacgaccg cttcggccag atgacggcgg ttcaccgcga
      241 ggaaggtatc agcctttacc gccactatga caaccgtggc cggttaacct cggtgaaaga
      301 cgcacagggc cgtgaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
      361 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cggtc
//
LOCUS       DAZKGS010000651          414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZKGS010000651 DAZKGS010000000
VERSION     DAZKGS010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHOQY_005617"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHOQY_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           97 a          110 c           86 g          121 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atct
//
LOCUS       DAZKGS010000652          413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZKGS010000652 DAZKGS010000000
VERSION     DAZKGS010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>413)
                     /locus_tag="ACHOQY_005618"
     CDS             complement(<1..>413)
                     /locus_tag="ACHOQY_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9681451.1"
                     /translation="NRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDP
                     DWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVG
                     PTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPV"
BASE COUNT          126 a           82 c           69 g          136 t
ORIGIN      
        1 tacaggttta agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact
       61 acgtgacacc ggatagtcag cccactcatg ccagggtttt gtaggtccca catagtgtat
      121 aaacacggtg tcatcattga ctggattatc aactttgtct tttaattcat agttaatact
      181 atatcgggta ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag
      241 tacatcttga tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat
      301 agcttttgac gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc
      361 attgaaatat ccagaagcta actgtggtgt agttaatacc gaggctcgat ttt
//
LOCUS       DAZKGS010000653          407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZKGS010000653 DAZKGS010000000
VERSION     DAZKGS010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..241)
                     /locus_tag="ACHOQY_005619"
                     /pseudo
     CDS             complement(1..241)
                     /locus_tag="ACHOQY_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
BASE COUNT          129 a           72 c           50 g          156 t
ORIGIN      
        1 cccattatgt cattgtgggt tgatgttggg ctttcataaa aaaaacatct ttttttgaac
       61 tagcattaat tatatttact ttatatatta aaatattttc tccatctgga aatattttag
      121 ccacacaata taaagcagct gaatccgaat tacttaaaaa tatattatca tcacccataa
      181 ctccagccat taaatgactg gagactaaag caaggaaata acaacattgt attgccttca
      241 ttttaattac tcccttgcct gataattgta actccattcc ctttagcttt gggattatat
      301 tttagatagg tgttatttgg tgttcccaag taactactgt attctttttt tcccattcca
      361 ttcatataag aattcgttaa atctttgctt gaaaaattta gactatc
//
LOCUS       DAZKGS010000654          406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZKGS010000654 DAZKGS010000000
VERSION     DAZKGS010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.657"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..346)
                     /locus_tag="ACHOQY_005620"
     CDS             complement(56..346)
                     /locus_tag="ACHOQY_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9681452.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT           86 a          118 c           84 g          118 t
ORIGIN      
        1 ataaaaaggc ctgcgattac cagcaggcct gttattagct cagtgatgta gatggtcatc
       61 ttttaactcc atataccgcc aatacccgtt tcatcgcggc actctggcga cactccttaa
      121 aaattaggtt cgtgctcatc tttccttccc gttcttcctt ggtagcaaac cggtaataca
      181 ccgttcgcca gaccttacct tcgataacca gaagacctgc ccgtgccatt ttagccgcgg
      241 cctgatttat gctggttact gttgcgcctg ttagcgcggc aacgtccggc gcacagaagc
      301 tattatgcgt ccccaggtaa tgaataattg cctctttgcc cgtcatacac ttgctccttt
      361 cagtccgaac ttagctttga gttctgcgat cttcgccaga gcctgt
//
LOCUS       DAZKGS010000655          398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZKGS010000655 DAZKGS010000000
VERSION     DAZKGS010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.658"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..380)
                     /locus_tag="ACHOQY_005621"
     CDS             complement(<1..380)
                     /locus_tag="ACHOQY_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HFP9681453.1"
                     /translation="MDKDNLFNDLNKLNGYLDSLDERGLILSLAAFSEDALGKMLLTF
                     MLDNKASKELIEGFNAPLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKFSHSWEN
                     ISIEDQDISQQIKALSFSRIDFECP"
BASE COUNT          119 a           75 c           67 g          137 t
ORIGIN      
        1 gggcattcaa aatcaatacg actgaagctt aacgctttaa tttgctgaga aatatcctga
       61 tcctcaattg aaatgttttc ccagctgtga gagaatttat ttcttatttt tctaagaagt
      121 tcaagatctt tgtattgacc ctctgttatt agtcccaagg aaaaacaggc ttttattcgt
      181 gaagaaaatg ttccaagggg ggcattaaac ccttcgatta attctttaga cgctttgtta
      241 tcgagcatga acgtcaaaag cattttacct aaggcgtctt cagagaaagc cgctagagac
      301 aatattaggc ctctttcatc taggctgtcc aaataaccgt tcagtttgtt tagatcatta
      361 aataaattat ctttatccat ttcttctgcc ttataaat
//
LOCUS       DAZKGS010000656          393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZKGS010000656 DAZKGS010000000
VERSION     DAZKGS010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.659"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>393
                     /locus_tag="ACHOQY_005622"
     CDS             <1..>393
                     /locus_tag="ACHOQY_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9681454.1"
                     /translation="KRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRN
                     SRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTV
                     FGPVNSFQKNQKTLARLQRQLSRKVKFS"
BASE COUNT          106 a           96 c          108 g           83 t
ORIGIN      
        1 gaagcgggct gcttttcccc gattcaaaaa gcggggacag aatgatgcat tccgctaccc
       61 gcagggcgta aagctcgatc aggaaaacag ccgtattttt ctgccgaaac tgggctggat
      121 gcgctaccgg aatagccgtc aggtcacggg tgttgtgaaa aatgtcacgg tcagccagtc
      181 ctgcggtaag tggtacatca gtattcagac agaaagtgaa gtatcaactc cggttcaccc
      241 ttcagcatca atgatcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg
      301 cacagtcttt gggcctgtaa acagttttca gaaaaaccag aagacgctgg cgagacttca
      361 gcgacagtta agccgcaagg tcaaattcag caa
//
LOCUS       DAZKGS010000657          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZKGS010000657 DAZKGS010000000
VERSION     DAZKGS010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.660"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          132 a           61 c           91 g          107 t
ORIGIN      
        1 atcaaggtga tatagcctat atgcctttga tgtggaggaa tgaatgtgat gggagtgatg
       61 tatctgaata gttgaaaaac cgcagacaca ccttatgcaa gaacgtgctg cggttggctg
      121 gtaaattttt cgatagtgcg agtattgaat gatttccagc cgttattgat tttacgcgta
      181 aatccatgaa aaaactactt atctgttggg gagttttttg gggcatatat gggacagaaa
      241 taggccccag atagacactg agatcgaact taggatgctt ttaaaaaaat gcaactatct
      301 gaaaaaacct agaaaacgcc aaggaaacca caggatggga aaaaacacct gtgaattatg
      361 gatttcaaga gtcattcaat ctaactaatt g
//
LOCUS       DAZKGS010000658          391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZKGS010000658 DAZKGS010000000
VERSION     DAZKGS010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.661"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..390)
                     /locus_tag="ACHOQY_005623"
     CDS             complement(<1..390)
                     /locus_tag="ACHOQY_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HFP9681455.1"
                     /translation="MSINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKLGFII
                     SLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQNIFE
                     TNAGEMTSYFNMSVVLWFLFTGILPCGL"
BASE COUNT          170 a           62 c           56 g          103 t
ORIGIN      
        1 tagtccacat ggaagtatac ccgtaaataa aaaccacagt acaacagaca tattgaaata
       61 tgatgtcatt tcacctgcat ttgtttcaaa aatattctgc atcattgttt tatcaaactg
      121 cacaccatat ttcatggtgg catatgtaac aatggaacta cagataaaca gaatacaaaa
      181 aaaaggttta aatatatacc tgaatgagaa cggtgtaaaa acaaaattta atgcagatat
      241 cagaaagact ggaagagata taataaatcc aagtttcaca ccatcaaggt tattaataat
      301 attaaacaga tgcatccaga cagggatatt gaacaatgtg gcaaaaacaa catctatcat
      361 taacatcaag aaaagaaaat taatactgat t
//
LOCUS       DAZKGS010000659          390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZKGS010000659 DAZKGS010000000
VERSION     DAZKGS010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.662"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..359)
                     /locus_tag="ACHOQY_005624"
     CDS             complement(<1..359)
                     /locus_tag="ACHOQY_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681456.1"
                     /translation="MPLYEWLINKRLRYQYLTLLAFSILALLALYLLYRNTPKVSVNF
                     FEFYHKNLRGYLFSGFISVGSFLLSLHTFVIINLRDKVFATQEYKEIYSIATGIPIDK
                     INDSVLYKPLDNLSSFI"
BASE COUNT          134 a           62 c           68 g          126 t
ORIGIN      
        1 ttaataaatg aggataaatt gtctaaaggt ttataaagta cactatcatt gattttgtca
       61 ataggtatac cagtggcaat gctataaatt tccttatatt cctgagttgc aaaaacctta
      121 tcgcgtaaat ttattatgac aaaggtatgc aggctcaata aaaatgagcc cacggaaata
      181 aaaccggaga agagatagcc tcgtaagttt ttatgataaa actcaaaaaa gttaacactt
      241 actttaggtg tgtttctgta caataggtaa agtgcaagca atgccaggat cgagaatgca
      301 agcagtgtaa ggtactgata cctcaatctt ttatttatta gccattcata taaaggcatt
      361 tttattcgtc ccgttcagca tttcttcttt
//
LOCUS       DAZKGS010000660          388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZKGS010000660 DAZKGS010000000
VERSION     DAZKGS010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.663"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>388)
                     /locus_tag="ACHOQY_005625"
     CDS             complement(<1..>388)
                     /locus_tag="ACHOQY_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9681457.1"
                     /translation="GMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAAIERI
                     EVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSS
                     GPLVDDSVSLQVRGSTQQRQGSSVTSL"
BASE COUNT           92 a          112 c           93 g           91 t
ORIGIN      
        1 tcagtgatgt gaccgatgag ccctgacgct gttgtgtgct accgcgtacc tgcaggctga
       61 cagaatcatc cacaagggga ccactgctcc agaaattaaa ctggctgctg ttaccccatt
      121 tgttgctttc ctgcagattc agccctgcat tgacggaaga gagccatttg tctgcattct
      181 ttctggtaat gatattcacc acaccgccca tcgcatcaga gccatacagt gtggacatcg
      241 gccccctgat aacctcaata cgctcaatgg cggccagagg gggcatgaac ccggtattca
      301 tggcagaaaa accgttggga gtcacgtcac tgcttccgcc ctgacgaaca ccatcaatca
      361 gtatcagcgt gtaactggct ggcattcc
//
LOCUS       DAZKGS010000661          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZKGS010000661 DAZKGS010000000
VERSION     DAZKGS010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.664"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..170)
                     /locus_tag="ACHOQY_005626"
     CDS             complement(<1..170)
                     /locus_tag="ACHOQY_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9681458.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQ"
BASE COUNT          102 a          105 c           80 g           99 t
ORIGIN      
        1 cactgatagt ctgccagacc cgccataaca agctgggtca gtattaactg gcagcgttcg
       61 cgtgaaaggt aagtattctg cgcaatctcc ccgactgtcg ccggttcggt gacgcttaat
      121 tcatcaaaca caactctggc ggtttctgtc atatcctgct gtttcagcat gtctttttac
      181 cctttccggt taacgtgaca caccaataac tcttgccgaa aaagccagca agctgaaaga
      241 cctgtattaa taactaccag cacatttaac gcactgcgct actttgcggg cacaaaaaac
      301 ccgctcagag gcggggtcaa gctatgcggc gaaataacca ctcttaacag catacctgat
      361 tttttacgta cgtaaatgct ttgccg
//
LOCUS       DAZKGS010000662          384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZKGS010000662 DAZKGS010000000
VERSION     DAZKGS010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.665"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>384
                     /locus_tag="ACHOQY_005627"
     CDS             <1..>384
                     /locus_tag="ACHOQY_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFP9681459.1"
                     /translation="SRVFFHGWDNKSIQYVTEINKDLIKDINLLLTSSNVDVKLLIKL
                     DRELYAISSKIENPLALRSIRTLQLQLTNYVTSNTFEPENTINFIYDFYSKKQNDLLS
                     AIKLFSRNDVETKIIVWYNSTMEKNV"
BASE COUNT          148 a           49 c           57 g          130 t
ORIGIN      
        1 tcgcgagttt tttttcatgg atgggataat aaaagcatac aatatgttac tgagattaat
       61 aaagacctta ttaaagatat aaatttattg ttaacatcgt ctaatgttga tgttaaactt
      121 ttaataaagc tagatcgtga attatatgca ataagcagca aaatagagaa tccgttagcc
      181 ttgcgctcta taaggacttt acagctgcaa ttaactaatt atgtaacctc aaacacattt
      241 gaacctgaaa atacaattaa ttttatttat gatttttata gtaaaaaaca aaatgattta
      301 cttagcgcaa ttaagttatt ttcgcgtaat gatgtcgaga caaaaatcat tgtttggtac
      361 aattcaacaa tggaaaaaaa tgta
//
LOCUS       DAZKGS010000663          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZKGS010000663 DAZKGS010000000
VERSION     DAZKGS010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.666"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>378
                     /locus_tag="ACHOQY_005628"
     CDS             <1..>378
                     /locus_tag="ACHOQY_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9681460.1"
                     /translation="PEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLAN
                     PGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDR
                     RVALIRQQAAEQNKVYQSMLVMN"
BASE COUNT          100 a           89 c          120 g           69 t
ORIGIN      
        1 tgcctgaagc ctcagataat gaaggacgaa cacgggcggc gctgacagag cagaaccggc
       61 tgattgatga acaggccagt cgggtgaaat ccctgcagga aaaaatcgca ggatatcagt
      121 atgttctggc gaacccgggc tggacgaccg gtgacggatt catgataaac catctgacct
      181 cggtgaagac cgtaacggaa gggcttgctc aggcaacaga gcagcttgcc gttgagcagt
      241 cccgtctggc acagatgcag gaaaaagcgc agtccattca ggatgtgctt gccgggctgg
      301 aagaccgtcg tgtggcgtta attcgtcagc aggcggcaga gcagaataag gtgtaccagt
      361 ccatgctggt tatgaacg
//
LOCUS       DAZKGS010000664          376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZKGS010000664 DAZKGS010000000
VERSION     DAZKGS010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.667"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>376
                     /locus_tag="ACHOQY_005629"
     CDS             <1..>376
                     /locus_tag="ACHOQY_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP9681461.1"
                     /translation="FKNIEMAIADIKKEYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLREL"
BASE COUNT          147 a           64 c           62 g          103 t
ORIGIN      
        1 tctttaaaaa catagagatg gctatagcag atataaaaaa ggagtactat tcgcatacag
       61 ttgatattga gaaaaatatt catgccatat gggttgcggg ttccccacct gaaagcatct
      121 cggactatat taagactttt ctcaaaacct acaaagagtt tacttactat ctttgggttg
      181 atgaaaaagc atttggagcc gcgaaattta ccagtgtctt aaaacaaatt gcatttgatt
      241 tagcatgcag aactatacag cagaatactc cacaaaaaaa tattgatttt attaatctat
      301 ataatgaaat aaggaagaaa tacaacaaca acccatcggg acaacaagag tacttaaaca
      361 aactcagaga gcttta
//
LOCUS       DAZKGS010000665          375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZKGS010000665 DAZKGS010000000
VERSION     DAZKGS010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.668"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..346
                     /gene="escD"
                     /locus_tag="ACHOQY_005630"
     CDS             <1..346
                     /gene="escD"
                     /locus_tag="ACHOQY_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP9681462.1"
                     /translation="YVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILFQN
                     IQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINIS
                     RPDEYIHIPLSY"
BASE COUNT          121 a           77 c           72 g          105 t
ORIGIN      
        1 ctatgtcaat attttcaaaa agaatgatgt catcattgtt gcgggggagg tttctcaaca
       61 gaacgagagt aagatccttg ccattataaa tgcgatgaac aaaaatagta acgtcaagat
      121 actatttcag aacattcagc cgtacatttc agcagatatt ttccccggaa aaatactcag
      181 aatcagcggc acgatgaaga atccaacaat tgctcttgat aacggtacaa gcctgggaat
      241 tggtagcatt ctgaaaggag gctatgtcat agacgcgatt gatcctaaag atggtatcaa
      301 catctcccgc ccagacgaat atatacatat tccgttgtct tattaactta ttattatctt
      361 ccccctcttc ggagg
//
LOCUS       DAZKGS010000666          373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZKGS010000666 DAZKGS010000000
VERSION     DAZKGS010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.669"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           92 a           92 c           88 g          101 t
ORIGIN      
        1 tagccgttcc cctatcatca tgatagtaag ccgtatgttt agatggttta tgtctgagat
       61 tttgagtatc aaatctttgg gcctcgtaaa aaggtttgaa tcgctaagga taatctggac
      121 gtctccaggg tcgttaatta gactgacccc accccgatag acgatcactc ccctatagta
      181 ataagcacca tttcggactt ccgcttctcg ctcacaacgg accttcagct cagccgctcc
      241 ggccgcttca tgcaatgaat gtacgcagcc agtcagaata ttaaggtgcg gatttcagtt
      301 cacttgtcag ccctgtgatg caacaagttg cggctccggt tcgtgggaga gatgaaacga
      361 cggtagctga agt
//
LOCUS       DAZKGS010000667          369 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZKGS010000667 DAZKGS010000000
VERSION     DAZKGS010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.670"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>369
                     /locus_tag="ACHOQY_005631"
     CDS             <1..>369
                     /locus_tag="ACHOQY_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681463.1"
                     /translation="FRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRL
                     AGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGV
                     AQMLAPKAKTPDYRATDNGRQ"
BASE COUNT           72 a           97 c          124 g           76 t
ORIGIN      
        1 tttcgccgtc agatgaacga aggctggtac cagatacgta ttgccggtga tgacacggca
       61 ccggaggcgg tgtacgcccg tcttcacgaa cagctgggtg agggaacggt catccatatt
      121 gtgccgcgac tggccggggc cggaaagggt ggactgcaga ttgtgctggg ggcggcagcc
      181 atcgtgggct ctttcttcac tgccggggca tcaatggcgt tatggggttc agccctggca
      241 gccggtggtt tttctgccac cacaatgctg ttttcacttg gagccagcat gattctgggc
      301 ggtgtggccc agatgctggc cccgaaggca aaaacaccgg attaccgcgc aacggataac
      361 ggcagacag
//
LOCUS       DAZKGS010000668          364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZKGS010000668 DAZKGS010000000
VERSION     DAZKGS010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.671"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>364)
                     /locus_tag="ACHOQY_005632"
     CDS             complement(<1..>364)
                     /locus_tag="ACHOQY_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP9681464.1"
                     /translation="YLFMSSGAVTARNVQTFSPTINNESRIRLPRALPVGHPYPEEHK
                     ASGFSPFMMGGLSGDILPEILTGNGPSIFFNGKHNNQHDGAFGKIIDFTQNGNKISAK
                     DKEIIKRYIFDKINVLIK"
BASE COUNT          107 a           73 c           54 g          130 t
ORIGIN      
        1 tctttaatca aaacattgat cttatcaaaa atatatcttt ttattatttc tttatctttt
       61 gcacttattt tatttccatt ttgggtaaaa tctattattt ttccaaaagc tccatcatgt
      121 tggttattat gttttccgtt aaaaaatata gatggtccat tccccgttaa aatttccgga
      181 agaatatcac cactcagccc ccccatcata aaaggggaga aaccagaagc cttatgttcc
      241 tcaggatatg gatggcctac tggcaatgct ctcggcaatc gaatccttga ctcattattt
      301 attgttggag aaaaggtttg aacatttctg gctgtcaccg ctcctgatga cataaacaaa
      361 tatg
//
LOCUS       DAZKGS010000669          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZKGS010000669 DAZKGS010000000
VERSION     DAZKGS010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.672"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..197
                     /locus_tag="ACHOQY_005633"
     tRNA            122..197
                     /locus_tag="ACHOQY_005633"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT          102 a           82 c           83 g           93 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
//
LOCUS       DAZKGS010000670          359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZKGS010000670 DAZKGS010000000
VERSION     DAZKGS010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.673"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..68)
                     /locus_tag="ACHOQY_005634"
     CDS             complement(<1..68)
                     /locus_tag="ACHOQY_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9681465.1"
                     /translation="MVTVAELQALRQARLDLLTGKRV"
     gene            complement(52..>359)
                     /locus_tag="ACHOQY_005635"
     CDS             complement(52..>359)
                     /locus_tag="ACHOQY_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9681466.1"
                     /translation="YAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRR
                     NEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
BASE COUNT           72 a          118 c           81 g           88 t
ORIGIN      
        1 acccgtttac cggttaataa atcaaggcgc gcctgacgca gcgcctgcag ttcagcgact
       61 gtaaccattc actcctccgg acagcttcgc tgccagttct ttcagggttg gccgggtcgt
      121 ctcttcttcc cgggattttg ccagtacagc cagatcaagc tgccagcgtt gcacggacac
      181 acgtaatgcc gcgtaggcat acaccaggca gtccagcgct tcgttacgcc gctttttgtt
      241 atcccacagc agacgcatct ttcctttttc ccacttctcc accagctctt ccgccaccag
      301 ttgctgcgcc tctgtctgcg aaaaaatctc cggatcatca ggaaaacgga tggcatacg
//
LOCUS       DAZKGS010000671          356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZKGS010000671 DAZKGS010000000
VERSION     DAZKGS010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.674"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHOQY_005636"
     CDS             <1..>356
                     /locus_tag="ACHOQY_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9681467.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZKGS010000672          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZKGS010000672 DAZKGS010000000
VERSION     DAZKGS010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.675"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..312)
                     /gene="ycgZ"
                     /locus_tag="ACHOQY_005637"
     CDS             complement(76..312)
                     /gene="ycgZ"
                     /locus_tag="ACHOQY_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP9681468.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          101 a           76 c           62 g          113 t
ORIGIN      
        1 atattttata cgttcattat cagatgtctt cataacgact tcatagctaa cctgttaaat
       61 aatcaggctg ttgcattatt caaaaagcaa cccaattagt gcattataat gtttctgttc
      121 ctcttcccca gtcgcgtgtt ccagtcggca aagaagtttg gcacaaagcg actttcgact
      181 cagattacgt ccatctttca aaatctcagt cacaatttcc ccgagagttt cctgctgagt
      241 aggcaagtta gcttttgcaa agtaacgagt gattgctccc gcagaatcta aagtcactga
      301 attttgatgc atgctacgcc tctgttaaaa atgttaatca atgttaagtg ag
//
LOCUS       DAZKGS010000673          346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZKGS010000673 DAZKGS010000000
VERSION     DAZKGS010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.676"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>346
                     /locus_tag="ACHOQY_005638"
     CDS             <1..>346
                     /locus_tag="ACHOQY_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9681469.1"
                     /translation="LNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFSL
                     ALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFSEE
                     FKQIAITDIPLR"
BASE COUNT          116 a           60 c           66 g          104 t
ORIGIN      
        1 gtttaaacgc gcttgcacag ttaggtatta gtgttgagat aatatctcat gaacttgaag
       61 aaatggattc tatggtaacc agaggactaa actctcttcc tacttctgta aaagaacacc
      121 ctggtttttc attggcgtta aatgctcaca gatcgcttac tcaacaaata cgtttcttat
      181 cacctttgaa aatatcaggt tatcaatcca ggcagagaat aactggaaaa aatatcatgg
      241 attatgtcct gaagttcttt ggggagcgtt tcgaacggca acgaataact attgaattta
      301 gtgaagagtt taagcaaatc gcaataacag atataccatt aaggat
//
LOCUS       DAZKGS010000674          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZKGS010000674 DAZKGS010000000
VERSION     DAZKGS010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.677"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..248
                     /locus_tag="ACHOQY_005639"
     CDS             <1..248
                     /locus_tag="ACHOQY_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681470.1"
                     /translation="HSTNNISADITLTRTPHITEFSSDSITAQIKKNGINIQTGRMVS
                     QKHLIITHDQEGKHLTTWYQQISLRVHSYKQHTEEVR"
BASE COUNT          139 a           83 c           45 g           76 t
ORIGIN      
        1 cacactcaac taataatatc tcagcagata ttaccctgac caggacacct cacattactg
       61 aattctcttc cgacagtatc actgcacaaa taaaaaagaa cgggataaat attcagaccg
      121 gaagaatggt gtcacaaaag catttaatca ttacgcatga ccaggaaggg aaacacttaa
      181 ccacatggta tcaacagata tcattacgtg tgcactctta taaacaacac acagaagaag
      241 taagataatt ataaacgtaa atttaatatc tcccactgac atctccccca caatacactg
      301 aaaaatactc acagcagtaa acacagacac acctgataat cat
//
LOCUS       DAZKGS010000675          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZKGS010000675 DAZKGS010000000
VERSION     DAZKGS010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.678"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..162
                     /locus_tag="ACHOQY_005640"
     CDS             <1..162
                     /locus_tag="ACHOQY_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP9681471.1"
                     /translation="AVCCLFDFDAFSRLASEGSYHPLTREPITASMIISPDKCVYDPI
                     KGNFIIKDS"
BASE COUNT          107 a           51 c           71 g          112 t
ORIGIN      
        1 gcagtatgct gcttatttga ttttgatgca ttttctcgtt tagctagtga aggctcatat
       61 catccactga cccgagaacc aataacggca tcaatgatta taagtcctga taaatgtgtt
      121 tatgatccta tcaagggaaa cttcattata aaagatagtt aaaatatttt tctctgagag
      181 agtattggct gtcagtaatt tgtaaaaaaa cgccgcagac atccagaata taagaaggtg
      241 tctgtggttg gcaggtttca gatgacttga aggcgtcttt atgtaaatgc atttttctgt
      301 ggagatgttg tcggaaaata ttggattaaa aacataatcc a
//
LOCUS       DAZKGS010000676          339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZKGS010000676 DAZKGS010000000
VERSION     DAZKGS010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.679"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>339)
                     /locus_tag="ACHOQY_005641"
     CDS             complement(<1..>339)
                     /locus_tag="ACHOQY_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681472.1"
                     /translation="TPLFVAQGNQLFMNDVFLKRLFAASITSSGNPPTFSLTPEGKLT
                     ARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSYASG
                     TVTVTVYDDQG"
BASE COUNT           67 a           99 c           86 g           87 t
ORIGIN      
        1 ccctggtcat cgtaaactgt gacggttacc gtcccgctgg cataactgtt attccgggga
       61 aaggctttcc ccaccgtctt caccaggtcg ccttcaatct ggtttgcaga cagtttccct
      121 ctgatgacac agttctcgtt aatggtgaca ttattgagcg tgccggtatt cgcggtaatt
      181 gctccgctga tatccgcgtt cctggctgtc agcttccctt ccggcgtcag ggaaaacgtc
      241 ggggggttgc cggatgacgt gatactcgcc gcaaacagcc gcttcaggaa cacatcgttc
      301 atgaacaact gattcccctg cgccacaaat aacggcgtg
//
LOCUS       DAZKGS010000677          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZKGS010000677 DAZKGS010000000
VERSION     DAZKGS010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.680"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..205
                     /locus_tag="ACHOQY_005642"
     CDS             <1..205
                     /locus_tag="ACHOQY_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9681473.1"
                     /translation="VAAQKRAECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEER
                     RVFRRYKGTGEQNTSGNARSKKC"
BASE COUNT           98 a           62 c           91 g           82 t
ORIGIN      
        1 agtggcggca cagaaacgag cagagtgttt gcgtaaggcg cgccggactc cggtttcacc
       61 ggtgaacctg accgaaataa aacaagcggt caacagacta cattctgagt tgggaatggg
      121 atttgaagag cggcgggtat tccgacgata taaagggaca ggagaacaga atacgtccgg
      181 aaacgcgcgg tcaaaaaaat gctaaaaaat atctgagagc gttattgcct gttaccataa
      241 gaaaaagcga ctttagtggt cgctttttgt gtcatatata agtcgtttaa gtaaacctgt
      301 ctgaacaggt tctctggtcg tgtttgtctt tgt
//
LOCUS       DAZKGS010000678          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZKGS010000678 DAZKGS010000000
VERSION     DAZKGS010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.681"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..>325
                     /locus_tag="ACHOQY_005643"
     CDS             54..>325
                     /locus_tag="ACHOQY_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9681474.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYI"
BASE COUNT           95 a           72 c           83 g           75 t
ORIGIN      
        1 aatctttatt tcaagattaa agaagataag cgtaaggctg cgagaggtga ataatgtcgc
       61 cgtcattacg caaggctgtt gcagctgcta ttggtggtgg ggctgttgcc atagcgtctg
      121 tgctcatcac tggtccaggt ggtaacgatg gtctggaagg tgtcagctac ataccataca
      181 aagatatcgt tggcgtatgg actgtatgtc acggacacac cggaaaagac atcatgcccg
      241 gtaaaacgta taccgaagca gaatgcaaag ccctcctgaa taaagacctt gccacggtcg
      301 ccagacaaat taacccgtac atcaa
//
LOCUS       DAZKGS010000679          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZKGS010000679 DAZKGS010000000
VERSION     DAZKGS010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.682"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..284
                     /locus_tag="ACHOQY_005644"
     CDS             <1..284
                     /locus_tag="ACHOQY_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9681475.1"
                     /translation="KSLRVGDAKKFAARLGVSPSYLSQMASGRIAISPTRALMIESAT
                     EGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
BASE COUNT           84 a           64 c           87 g           86 t
ORIGIN      
        1 ttaaatcatt aagggttggt gatgctaaga aattcgcggc cagacttggt gtatcgccat
       61 cttacttatc gcaaatggcg tctggacgaa tagctatatc tccaacccgc gcccttatga
      121 tcgaatctgc gacggaaggc caagtaagta gggcggagct acgacctcat gattgggagc
      181 ttatttggcc tgagtatgcg agcggcattc gtttggggca aacacatgta gttcatgctg
      241 aaggtgattg tagtgcatgc ttatctgatg gagttgattc atgaaaatca agcatgaaca
      301 catccgcatg gcgatgaatg t
//
LOCUS       DAZKGS010000680          320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZKGS010000680 DAZKGS010000000
VERSION     DAZKGS010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.683"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            82..>320
                     /gene="grlR"
                     /locus_tag="ACHOQY_005645"
     CDS             82..>320
                     /gene="grlR"
                     /locus_tag="ACHOQY_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFP9681476.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVL"
BASE COUNT          102 a           46 c           73 g           99 t
ORIGIN      
        1 gaaaggtctc cgttattctt gatatcgctt atggatagaa caaattgaaa ggagtgaggt
       61 tagtatgaaa ctgagtgagt tatgattatg aaggatggca tctatagcat tatctttatt
      121 agcaatgaag attcctgtgg ggaaggtata ctgattaaaa atggaaattt gatcactggc
      181 ggagatattg cttctgtgta tcagggggtc ctgtctgaag aggaggacat catacttcat
      241 gtccatcgct ataatcatga aattccctcg gtgctaaaca ttgaacaaga ttatcaatta
      301 gttatcccta aaaaagtact
//
LOCUS       DAZKGS010000681          318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZKGS010000681 DAZKGS010000000
VERSION     DAZKGS010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.684"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>318
                     /locus_tag="ACHOQY_005646"
     CDS             <1..>318
                     /locus_tag="ACHOQY_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP9681477.1"
                     /translation="GYKNYHESLNAAIAINSRPLQEIHYGDVLYHYHKNDISLGVDTL
                     YGRESFDIVLDAMNVYRKSKKMRVISNNEMKKSIKISELVIHNIIKKGLTNCLLKKDV
                     LNA"
BASE COUNT          126 a           35 c           56 g          101 t
ORIGIN      
        1 gggttacaaa aattatcatg agtcattaaa tgctgctatt gcaataaact ctagaccatt
       61 acaagaaata cattatggtg atgttttata tcattatcat aaaaatgaca tctctttggg
      121 agtagatact ctttacggga gggaaagttt tgatattgta ctggatgcaa tgaacgtata
      181 tagaaaaagc aaaaaaatga gagttatttc caataatgag atgaaaaaaa gcattaaaat
      241 atctgaatta gttatccata atattataaa gaaaggattg actaattgtt tgcttaaaaa
      301 ggatgttctt aatgccag
//
LOCUS       DAZKGS010000682          316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZKGS010000682 DAZKGS010000000
VERSION     DAZKGS010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.685"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..266
                     /locus_tag="ACHOQY_005647"
     CDS             <1..266
                     /locus_tag="ACHOQY_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9681478.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
BASE COUNT           62 a           84 c           90 g           80 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttg
//
LOCUS       DAZKGS010000683          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZKGS010000683 DAZKGS010000000
VERSION     DAZKGS010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.686"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..213
                     /locus_tag="ACHOQY_005648"
     CDS             28..213
                     /locus_tag="ACHOQY_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9681479.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIEA
                     CRRRLSTERSGMNYIIK"
BASE COUNT          106 a           76 c           65 g           67 t
ORIGIN      
        1 atatagcttg tcaccagagg aaagaaaatg tcatcgaaaa accggccccg cagaacaaca
       61 acccgcaaca tccgatttcc aaaccagatg attgaacaaa ttaacatcgc tcttgaccag
      121 aaaggttcag aaaatttttc agcgtgggtt attgaagcct gcagaagaag gctgtcaaca
      181 gagcgttcgg gtatgaatta cataattaag taacatggtg ttcacagaac acacagttac
      241 cggacacatc agttttccat tcgtcccccg gcagtacagg cttcccgtct gacgggatag
      301 cctgaaaaaa caca
//
LOCUS       DAZKGS010000684          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZKGS010000684 DAZKGS010000000
VERSION     DAZKGS010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.687"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>314
                     /locus_tag="ACHOQY_005649"
     CDS             <1..>314
                     /locus_tag="ACHOQY_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9681480.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZKGS010000685          312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZKGS010000685 DAZKGS010000000
VERSION     DAZKGS010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.688"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>312
                     /locus_tag="ACHOQY_005650"
     CDS             <1..>312
                     /locus_tag="ACHOQY_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFP9681481.1"
                     /translation="KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKY
                     FDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQT
                     IL"
BASE COUNT          103 a           54 c           51 g          104 t
ORIGIN      
        1 taaatttata gctgatatac agtttaatac tgacttatgt ggtgacactt atatttccag
       61 aggtcccgtt ttatatgcaa ttgagcttga gagcgatatt ttaatcaaaa aaactctgct
      121 gaacgggaaa tattttgact caggatacat accctgtagt agagctgatg aatctatcca
      181 gttctcttat gctgatatgg aatctttttc atattcaacc tcaccagagg gcaaacaata
      241 cattgaaggc tgtttttatt taaacaagac taaaatagtc agaaaactta taccatttgg
      301 tcaaaccatt ct
//
LOCUS       DAZKGS010000686          310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZKGS010000686 DAZKGS010000000
VERSION     DAZKGS010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.689"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..105
                     /locus_tag="ACHOQY_005651"
     CDS             <1..105
                     /locus_tag="ACHOQY_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681482.1"
                     /translation="ELEPWTPEGNAPNYCWWEVDPVDAANEAMSYHND"
     gene            138..>310
                     /locus_tag="ACHOQY_005652"
     CDS             138..>310
                     /locus_tag="ACHOQY_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9681483.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWAS"
BASE COUNT           93 a           57 c           77 g           83 t
ORIGIN      
        1 gaattagaac cgtggacacc tgaaggaaac gcaccaaatt attgctggtg ggaggttgat
       61 ccggttgatg ctgcaaatga agccatgagt taccacaacg attaatgtcg ggaggccgcc
      121 cgaaagggcg gtaagaaatg actacattat tcagaaaaga atatccgcaa aaaagtaggg
      181 cgacagaatt tttgtttctc attctgttta tcgtattgat gataccgata tcccctctaa
      241 tttttgtctg ggcaatcggg aaaataattg agccagttac tgaattgtat accgacgttg
      301 tatgggcgtc
//
LOCUS       DAZKGS010000687          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZKGS010000687 DAZKGS010000000
VERSION     DAZKGS010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.690"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>309)
                     /locus_tag="ACHOQY_005653"
     CDS             complement(<1..>309)
                     /locus_tag="ACHOQY_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681484.1"
                     /translation="IYLNTDFDLHFLKNEICEALNRHVRVIVFSFNKLITPDPRVELY
                     YRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLLINIIAEHIHSDIYLT
                     "
BASE COUNT          110 a           48 c           57 g           94 t
ORIGIN      
        1 tggttagata tatatcacta tggatatgtt cggcaattat attgataagc aagcgattat
       61 tcgagtataa ccccagtgat tcagctcgtt gtgaaaaaac cattactgtc ttcatatcaa
      121 caacaagcat aaaacggtga gaagggtaat attcttcttc atggttaaag cggtaataca
      181 gttctacacg tgggtcggga gtaataagtt tattaaatga aaaaacaata actcgaacat
      241 gtcgattcag agcctcacaa atttcatttt tcaaaaaatg taagtcaaaa tccgtgttga
      301 ggtaaattt
//
LOCUS       DAZKGS010000688          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZKGS010000688 DAZKGS010000000
VERSION     DAZKGS010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.691"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHOQY_005654"
     CDS             complement(<1..242)
                     /locus_tag="ACHOQY_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9681485.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           91 a           60 c           66 g           92 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgc
//
LOCUS       DAZKGS010000689          306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZKGS010000689 DAZKGS010000000
VERSION     DAZKGS010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.692"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>306
                     /locus_tag="ACHOQY_005655"
     CDS             <1..>306
                     /locus_tag="ACHOQY_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681486.1"
                     /translation="QQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQELLRNAAL
                     IDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFRQK
                     "
BASE COUNT           92 a           74 c          101 g           39 t
ORIGIN      
        1 cagcagaaag agcaactggc actggcgggt cagaatacag agctggcgaa gctgaagtac
       61 cagactgcgc agggcgaact gaaaaccctg acggagatgc agaagcagga actgctgcgt
      121 aacgcggccc tgattgacca gcaaaaaatc cgggaacagt tgcgatcccg ggaagagacc
      181 ctgaagaatg ataatgtggc tgcgcgtgca tcaaatgaag ccgaactgct gggatacggg
      241 cagggagaac gagccaggga acgcatgcgg gagttgcagc agatccgcga cagcttccgc
      301 cagaag
//
LOCUS       DAZKGS010000690          306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZKGS010000690 DAZKGS010000000
VERSION     DAZKGS010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.693"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..175
                     /gene="sctF"
                     /locus_tag="ACHOQY_005656"
     CDS             2..175
                     /gene="sctF"
                     /locus_tag="ACHOQY_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     subunit SctF"
                     /protein_id="HFP9681487.1"
                     /translation="MSDSVPELLNSTDLVNDPEKMLELQFAVQQYSAYVNVESGMLKT
                     IKDLVSTISNRSF"
     gene            181..>306
                     /locus_tag="ACHOQY_005657"
     CDS             181..>306
                     /locus_tag="ACHOQY_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFP9681488.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKK"
BASE COUNT           93 a           56 c           65 g           92 t
ORIGIN      
        1 gctgagtgat tctgtgccag agttacttaa tagcaccgat ttggttaatg accctgaaaa
       61 aatgttagag ctgcagttcg cggttcagca atattctgct tatgttaacg tagaaagtgg
      121 aatgctgaaa acgataaaag atttggtctc aaccatttcc aaccgtagct tttaattttt
      181 atggttaatg atgtttctgc caacaagata ctggtgtggg cggctgtagc tgcggcaaat
      241 cataagcttc ccaaatatgc agaatctatc ctgaatgtat tgccgcaaat tatacccgat
      301 aaaaaa
//
LOCUS       DAZKGS010000691          305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZKGS010000691 DAZKGS010000000
VERSION     DAZKGS010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.694"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..>305
                     /locus_tag="ACHOQY_005658"
     CDS             33..>305
                     /locus_tag="ACHOQY_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9681489.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVI"
BASE COUNT           66 a           78 c           73 g           88 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttata
//
LOCUS       DAZKGS010000692          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZKGS010000692 DAZKGS010000000
VERSION     DAZKGS010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.695"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>299
                     /locus_tag="ACHOQY_005659"
     CDS             <1..>299
                     /locus_tag="ACHOQY_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFP9681490.1"
                     /translation="CEIGLGANIGKGLTIPHHAGIVVHFAVDAGENLVLRQNTTIGQI
                     DGDMPGSRVKIGSNVDIGANCCIIGLSRKIGDNVKIGAMSFINKDIPSNCTYITK"
BASE COUNT           99 a           39 c           68 g           93 t
ORIGIN      
        1 gttgtgaaat tggattgggc gcaaatattg gaaaagggtt aacaattccc catcatgctg
       61 gaattgttgt tcattttgct gttgatgctg gtgaaaatct ggtgttacga cagaatacta
      121 ccattggaca gatagatggt gacatgcctg gttcaagagt aaaaattggt agtaacgttg
      181 atatcggagc taattgttgc atcattggat tatcacgtaa aattggggat aatgtaaaaa
      241 taggtgcaat gtcttttata aataaagata taccatcgaa ctgcacatat ataactaaa
//
LOCUS       DAZKGS010000693          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZKGS010000693 DAZKGS010000000
VERSION     DAZKGS010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.696"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>295
                     /locus_tag="ACHOQY_005660"
     CDS             <1..>295
                     /locus_tag="ACHOQY_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9681491.1"
                     /translation="LKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPK
                     RRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCRE"
BASE COUNT           84 a           79 c           76 g           56 t
ORIGIN      
        1 actgaagcag cgcacaaaaa atattgcgct accgcctcac acccaccagc aacagaaccc
       61 accacaggaa aagacggtgg tcagcattgc cgttgatccg gagtctccgg aatccttcat
      121 gaaacgacct aaacgtcgcc gctgggtaaa tgagaaatac acacgctggg taaagacaca
      181 gccgtgtgcg tgttgtggta agccagcgga cgatcctcat catctgattg gtcatggtca
      241 gggtggaatg ggaacaaaat cccacgatat tttcacgcta ccgctgtgtc gggag
//
LOCUS       DAZKGS010000694          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZKGS010000694 DAZKGS010000000
VERSION     DAZKGS010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.697"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>291
                     /locus_tag="ACHOQY_005661"
     CDS             <1..>291
                     /locus_tag="ACHOQY_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9681492.1"
                     /translation="LGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRK
                     AGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGL"
BASE COUNT           66 a           70 c           91 g           64 t
ORIGIN      
        1 ctggggattt atctgtatac ggatgctccc gttcctgatg gtgtggcgat gacgggtacc
       61 ggtaatccgt tcctgatgtc gtacttcact cagaccactg acggcagagt gaatctgatg
      121 catcacagga aagccggaaa cacgaagctg ggggagttcg gcgattacgg taacgactgg
      181 cagacgctgg agctggtgtt caccgccggc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggttataaaa gacagtctga cactgggact g
//
LOCUS       DAZKGS010000695          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZKGS010000695 DAZKGS010000000
VERSION     DAZKGS010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.698"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(177..>290)
                     /locus_tag="ACHOQY_005662"
     CDS             complement(177..>290)
                     /locus_tag="ACHOQY_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFP9681493.1"
                     /translation="AWVIESCRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT           86 a           48 c           55 g          101 t
ORIGIN      
        1 ggaatctgtt caataaaaaa actgccgtgt tggagtcaca gcagtaatgt actgattggg
       61 tagaagatta ttattgttat gctttatttt tattctatat ggctgattat ttcaattcgg
      121 aattaataca gctaatgtct gtgatttttt ataaattcag caatataaag aaatagttat
      181 atgaacagcc atcgcagagc atactgtgta tcattctttt ttatagtcaa ctgacgggca
      241 tattttatgt ctgctgccag ctcccggcgg caagattcaa tgacccacgc
//
LOCUS       DAZKGS010000696          289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZKGS010000696 DAZKGS010000000
VERSION     DAZKGS010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.699"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..>289
                     /gene="gpFI"
                     /locus_tag="ACHOQY_005663"
     CDS             2..>289
                     /gene="gpFI"
                     /locus_tag="ACHOQY_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9681494.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALH"
BASE COUNT           72 a           72 c           92 g           53 t
ORIGIN      
        1 catgacgaaa gatgaactga ttgcccgtct ccgctcgctg ggtgaacaac tgaaccgtga
       61 tgtcagcctg acggggacga aagaagaact ggcgctccgt gtggcagagc tggaagagga
      121 gcttgatgac acggatgacg cagccggtca ggacacgtct gtcagcccgg aaaatgcgct
      181 gaccggacat gaaaatgagg tggtatcagc acagacggat accgtgactg atacggctgc
      241 tctggtcacg gttgtggcac tggtgacgct gcataccgat gcacttcac
//
LOCUS       DAZKGS010000697          287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZKGS010000697 DAZKGS010000000
VERSION     DAZKGS010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.700"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>287
                     /locus_tag="ACHOQY_005664"
     rRNA            <1..>287
                     /locus_tag="ACHOQY_005664"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZKGS010000698          286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZKGS010000698 DAZKGS010000000
VERSION     DAZKGS010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.701"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..123
                     /locus_tag="ACHOQY_005665"
     CDS             <1..123
                     /locus_tag="ACHOQY_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681495.1"
                     /translation="SMFQTGSVAIRAERWINWRRRRTAAVAVITGVNYGTASGG"
     gene            168..>286
                     /locus_tag="ACHOQY_005666"
     CDS             168..>286
                     /locus_tag="ACHOQY_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681496.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITA"
BASE COUNT           53 a           75 c          107 g           51 t
ORIGIN      
        1 tccatgtttc agaccggcag cgtggccatc cgtgcggagc gctggatcaa ctggcgtcgt
       61 cgccgtaccg cggcagtggc ggtgatcacc ggtgtgaact acggaactgc gtccggtggc
      121 tgagtctgat gaggagggcg ggaggcgcga gcttcccgca gtaacagatg gcaaaaatcc
      181 agtatctgca gggcacgcat gatgcccggg ccggggatat ccgtgatgtg gcacagccgt
      241 gtgcggaggt gctggttcgc ctggggaagg cggagtacat cacagc
//
LOCUS       DAZKGS010000699          285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZKGS010000699 DAZKGS010000000
VERSION     DAZKGS010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.702"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..285
                     /locus_tag="ACHOQY_005667"
                     /pseudo
     CDS             1..285
                     /locus_tag="ACHOQY_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246436.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF943 family protein"
BASE COUNT           90 a           54 c           57 g           84 t
ORIGIN      
        1 gacattgcgt cctgtacaga ttttgtatac atactctgat gctggcaatt cagtctttct
       61 ggtcgttgat catctgccat ggactgacag cgataaaata aattggtatc tgaagcatca
      121 gaatgaaatt aaaaatcaac atcccctacc agaaggtagt tggcatacct ggtatgtcat
      181 tgatatcggt aatggcttca ctgactataa aaaaatatat cgaagggccg tatgaagatc
      241 tctactgttt cccgacaatt aagagcaatg ataattgcat cgtga
//
LOCUS       DAZKGS010000700          285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZKGS010000700 DAZKGS010000000
VERSION     DAZKGS010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.703"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>285
                     /locus_tag="ACHOQY_005668"
     CDS             <1..>285
                     /locus_tag="ACHOQY_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9681497.1"
                     /translation="DPEVLKQAVLEAVNALPAPQDGRDATALEVLPAIDDQKSFPRGT
                     YATHLGGLWRAYEKTHGMRGWECLVDGVADIDVSMTDERLFSVVIRQSSGR"
BASE COUNT           56 a           71 c          104 g           54 t
ORIGIN      
        1 gatccggaag tactgaagca ggcggttctg gaggcggtga atgcccttcc ggctccgcag
       61 gacgggcgtg atgccacggc actggaagtg ctccccgcta ttgacgatca aaaatccttt
      121 ccccggggca cgtatgccac acacctgggt ggactctggc gggcgtatga aaaaacgcac
      181 gggatgcggg gatgggaatg cctggttgac ggggtggcgg atatcgacgt cagcatgacg
      241 gatgagcggt tgttctctgt ggtgatccgg cagagcagtg gccgg
//
LOCUS       DAZKGS010000701          284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZKGS010000701 DAZKGS010000000
VERSION     DAZKGS010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.704"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>284
                     /locus_tag="ACHOQY_005669"
     CDS             <1..>284
                     /locus_tag="ACHOQY_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFP9681498.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZKGS010000702          284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZKGS010000702 DAZKGS010000000
VERSION     DAZKGS010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.705"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>284
                     /locus_tag="ACHOQY_005670"
     CDS             <1..>284
                     /locus_tag="ACHOQY_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681499.1"
                     /translation="HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASV
                     FWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHID"
BASE COUNT           57 a           84 c           75 g           68 t
ORIGIN      
        1 catctgactt atactcaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact
       61 tatgatattg ccgagggttc ggttgtcagt ctggcggccg gagggatcag ttttgccctg
      121 acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct
      181 gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactc
      241 caccggctca gtgttgttca gggtatgctg aagcacattg attt
//
LOCUS       DAZKGS010000703          281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZKGS010000703 DAZKGS010000000
VERSION     DAZKGS010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.706"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..182)
                     /gene="hokA"
                     /locus_tag="ACHOQY_005671"
     CDS             complement(30..182)
                     /gene="hokA"
                     /locus_tag="ACHOQY_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9681500.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT           83 a           63 c           55 g           80 t
ORIGIN      
        1 aggcgggaat aacttcccgc cgaagactct tactctttta aattgcaggc taaaaatgcc
       61 gccagctcat aactcccctg tttaatatgc aattcacaca gtgaatctct tatcatccag
      121 gtgaaaaata aaagcgtgaa acaaatcact attaaagaaa gtaatctata tttctgcggc
      181 attcccagcc ccctgtgttg atttccaacg agtaagaggc taaccttatg gtgttcaggc
      241 atagggtagc ctcactttga tttatagtca ggtggggctt t
//
LOCUS       DAZKGS010000704          273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZKGS010000704 DAZKGS010000000
VERSION     DAZKGS010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.707"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..95
                     /locus_tag="ACHOQY_005672"
     tRNA            19..95
                     /locus_tag="ACHOQY_005672"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHOQY_005673"
     tRNA            138..213
                     /locus_tag="ACHOQY_005673"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZKGS010000705          273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZKGS010000705 DAZKGS010000000
VERSION     DAZKGS010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.708"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..71)
                     /locus_tag="ACHOQY_005674"
     CDS             complement(<1..71)
                     /locus_tag="ACHOQY_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681501.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..>273)
                     /locus_tag="ACHOQY_005675"
     CDS             complement(68..>273)
                     /locus_tag="ACHOQY_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFP9681502.1"
                     /translation="MQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNV
                     VGLPVPDATGGRLEILCRLGGEK"
BASE COUNT           56 a          101 c           68 g           48 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcccct cccagccgac agagaatttc cagacgcccg ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccatggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccggc cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca ccggactgca tcc
//
LOCUS       DAZKGS010000706          271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZKGS010000706 DAZKGS010000000
VERSION     DAZKGS010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.709"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>271
                     /locus_tag="ACHOQY_005676"
     CDS             <1..>271
                     /locus_tag="ACHOQY_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP9681503.1"
                     /translation="KNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFE
                     ILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEMRKYLKSIN"
BASE COUNT          103 a           49 c           46 g           73 t
ORIGIN      
        1 aaaaaatttg ctctgtgaag atattatgcc tggtcgatgg accactcaac ttgtcaataa
       61 gcacttaggt tacagataca caggagtatt caaaaccctg gcaagtattg atgacaaacc
      121 gtcccgtttc gaaattttga taccattggt acaaacttta gttcgcgaca atgtaaagct
      181 aaataatgat gtgtataaag aacttaataa gtttatgcac gattacgaca aaacatcatc
      241 agaaatgaga aaatacctta aaagcatcaa c
//
LOCUS       DAZKGS010000707          271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZKGS010000707 DAZKGS010000000
VERSION     DAZKGS010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.denovo.710"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>271
                     /locus_tag="ACHOQY_005677"
     CDS             <1..>271
                     /locus_tag="ACHOQY_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9681504.1"
                     /translation="ANLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMS
                     KHANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGID"
BASE COUNT           55 a           64 c           90 g           62 t
ORIGIN      
        1 ggctaacctg ctgatggaaa acctcgccat ggtcaatccg aatctcggct attcagtgga
       61 tgaggccttt ctgtaccggg agtaccgtaa agcccgggaa gccggtgaag agacattccg
      121 ggggttcatg tcaaaacacg ccaatgtgga aattggtctt gccctgcgct ctgaccgctg
      181 ggcgggggct gatttctggg aagagcaggg ccgttgtatc agcctggacg atatcctgcg
      241 tcgtgctgat gtggtgacgg tggggattga c
//
LOCUS       DAZKGS010000708         6555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.guided.219, whole genome shotgun sequence.
ACCESSION   DAZKGS010000708 DAZKGS010000000
VERSION     DAZKGS010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.guided.219"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..443)
                     /locus_tag="ACHOQY_005678"
     CDS             complement(<1..443)
                     /locus_tag="ACHOQY_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9681505.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVV"
     gene            complement(662..1639)
                     /gene="epmA"
                     /locus_tag="ACHOQY_005679"
     CDS             complement(662..1639)
                     /gene="epmA"
                     /locus_tag="ACHOQY_005679"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9681506.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1964..3772
                     /gene="frdA"
                     /locus_tag="ACHOQY_005680"
     CDS             1964..3772
                     /gene="frdA"
                     /locus_tag="ACHOQY_005680"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9681507.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3765..4499
                     /gene="frdB"
                     /locus_tag="ACHOQY_005681"
     CDS             3765..4499
                     /gene="frdB"
                     /locus_tag="ACHOQY_005681"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9681508.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4510..4905
                     /gene="frdC"
                     /locus_tag="ACHOQY_005682"
     CDS             4510..4905
                     /gene="frdC"
                     /locus_tag="ACHOQY_005682"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9681509.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4916..5275
                     /gene="frdD"
                     /locus_tag="ACHOQY_005683"
     CDS             4916..5275
                     /gene="frdD"
                     /locus_tag="ACHOQY_005683"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9681510.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5338..6471
                     /locus_tag="ACHOQY_005684"
     CDS             5338..6471
                     /locus_tag="ACHOQY_005684"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9681511.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT         1680 a         1700 c         1685 g         1490 t
ORIGIN      
        1 acgacggtga ccagaatcat ccatgccact gccagcagac caaccacctg cgtaggctcc
       61 agtccagcaa tacgccagtg ctgggtcatg tcgctaccat agaggaatgt tgagaacggt
      121 acccaaactt tcgcggaggt actcaccatc cagatgatat aagaggaaaa ccacataaac
      181 gtaccaatga aggcaaaacg tggtccgaca ctattattca tccaggaata gataccgcct
      241 tcttctttgc gataagcagc tcccatttca gccatcatta aggcgaatgg aataaagaat
      301 aacaatgcag aaaatatata aaagggaatc gcactataac ccattaagta ataagccgat
      361 gggctattgg caaatccaaa tacggaagta aagatcatca atatgagtcc tatcagactc
      421 atctttttta tcgtgtgagg catgaaacca tccttcattt cagcgcgtgt ataacacgtg
      481 cccactgaac tgcactgcgc aacattgagc aggcagcgta aaaaccaata cagaattgat
      541 acctggatga tatcaaatat ccagtcttaa attgtggcct ttaacggaaa aatgaaaaca
      601 tatgctacag aatggcgctt atcacgccat tcttcgctgt taattcagta atttttcaga
      661 attatgcccg gtcaacgcta aaggcgatga cttcagccag tgtctccgcg cccagcgcca
      721 acatcaccag acgatcaaca cctaatgcca cgccggaaca gtcaggcata ccgactttca
      781 aggcttcaat cagattctgg tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac
      841 ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc cgtcaattca tggaaaccat
      901 tcgccagctc aataccttta taataaacct caaagcgttc agcgacccga tgatcttcgg
      961 tactgatttg cgccagtgat gcctggctgg ctggaaagtg gtacacaaag gtcggttttt
     1021 ctttgccaat atttggctct acgccaaagg taaacagcaa ttgtagcagc gtgtcgcggt
     1081 cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc tgcgacttcc cgcagttgcg
     1141 ttttgtcggc agagagcggg tcaatttcca gataacgcaa gaaagcttgt tgataagaaa
     1201 ggctttctgc tgccgggcag tccagcacct gttgtaagag atcgtccacc tcgttcatca
     1261 accggtacat atcatagtgc ggtcgatacc actccagcat agtgaactca gggttgtgat
     1321 aacgccccat ctcttcatta cggaagctgc ggcacagctg gaataccggc ccacaaccgg
     1381 caaccagcag gcgtttcata tggtattccg ggctggtcat taaccagaga ttcatcccct
     1441 gcgaatgccc ggggccaacg aaacgtgtct caaacgggac caaatgaata tcggttaccg
     1501 tcgcctggct catacaaggc gtctccacct ccagcactcc acgatcggca aagaaacgac
     1561 ggatctccgc cataatcgcc gcgcgtttta ataagttagg aatggatgcg ctcggctgcc
     1621 aggatgccgt ttcgctcata gttaaatctc cagtttttga caagggcacg aagtctactc
     1681 gcaacgcgac ggcgagacaa attttacgca ggaatcaaac agcggtgggc agtgactaaa
     1741 aaaagcacga tctgatggtt tagtaattaa attaatcatc ttcagtgata atttagccct
     1801 cttgcgcact aaaaaaatcg atctcgtcaa atttcagact tatccatcag actatactgt
     1861 tgtacctata aaggagcagt ggaatagcgt tcgcagaccg taactttcag gcacttaccc
     1921 tgaagtacgg ggctgtggga taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc
     1981 cgatcttgcc attgtaggcg ccggtggcgc gggattacgt gctgcaattg ctgccgcgca
     2041 ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta tacccgatgc gtagccatac
     2101 cgttgctgca gaagggggct ccgccgctgt cgcgcaggat catgacagct tcgaatatca
     2161 ctttcacgat acagtagcgg gtggcgactg gttgtgtgag caggatgtcg tggattattt
     2221 cgtccaccac tgcccaaccg aaatgaccca actggaactg tgggggtgcc catggagccg
     2281 tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc atgaaaatcg agcgcacctg
     2341 gttcgccgcc gataagaccg gcttccatat gctgcacacg ctgttccaga cctctctgca
     2401 attcccgcag atccagcgtt ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg
     2461 tcatgttcgc ggcgtggtag caatgaacat gatggaaggc acgctggtgc agatccgtgc
     2521 taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt tatcgttaca acaccaacgg
     2581 cggcatcgtt accggtgacg gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga
     2641 catggaattc gttcagtatc acccaaccgg tctgccaggt tccggtatcc tgatgaccga
     2701 aggctgccgc ggtgaaggcg gtattctggt caacaaaaat ggctaccgtt atctgcaaga
     2761 ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa aacaaatata tggaactggg
     2821 tccacgcgac aaagtttctc aggccttctg gcacgaatgg cgtaaaggca acaccatctc
     2881 cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac ctcggcgaga aaaaactgca
     2941 tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac gttggcgtcg atccggttaa
     3001 agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca
     3061 gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct
     3121 gcacggtgca aaccgtctgg gttctaactc cctggcggaa ctggtggtct tcggccgtct
     3181 ggccggtgaa caagcgacag agcgtgcagc aactgccggt aatggcaacg aagcggcaat
     3241 tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat ctggttaacc aggatggcgg
     3301 cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca atggaagaag gttgcggtat
     3361 ctaccgtacg ccggaactga tgcagaaaac catcgacaag ctggcagagc tgcaggaacg
     3421 cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc aacaccgacc tgctctacac
     3481 cattgaactg ggccacggtc tgaacgttgc tgaatgtatg gcgcactccg caatggcacg
     3541 taaagagtcc cgcggcgcac accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt
     3601 caacttcctc aaacacaccc tcgccttccg cgatgctgat ggcacgactc gcctggagta
     3661 cagcgacgtg aagattacta cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga
     3721 tgcagccgat aaggcggaag cagccaataa gaaggagaag gcgaatggct gagatgaaaa
     3781 acctgaaaat tgaggtggtg cgctataacc cggaagtcga taccgcaccg catagcgcat
     3841 tctatgaagt gccttatgac gcaactacct cattactgga tgcgctgggc tacatcaaag
     3901 acaacctggc accggacctg agttaccgct ggtcctgccg tatggcgatt tgtggttcct
     3961 gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca
     4021 ccgacggtat gaaggttgaa gcgttagcta acttcccgat tgaacgcgat ctggtggtcg
     4081 atatgaccca cttcatcgaa agtctggaag cgatcaaacc gtacatcatc ggcaactccc
     4141 gcaccgcgga tcagggtact aacatccaga ccccggcgca gatggcaaag tatcaccagt
     4201 tctccggttg catcaactgt ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc
     4261 cagagttcat cggtccggct gccattacgc tggcgcatcg ttataacgaa gatagccgcg
     4321 accacggtaa gaaggagcgt atggcgcagt tgaacagcca gaacggcgta tggagctgta
     4381 ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt cgatccggct gcggccattc
     4441 agcagggcaa agtagaaagt tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag
     4501 gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc caatgacgtc cacctggtgg
     4561 aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag gcacggcggt tccggctgtg
     4621 tggttcagca ttgaactgat tttcgggctg tttgccctga aaaatggccc ggaagcctgg
     4681 gcgggattcg tcgacttttt acaaaacccg gttatcgtga tcattaacct gatcactctg
     4741 gcggcagctc tgctgcacac caaaacctgg tttgaactgg caccgaaagc ggccaatatc
     4801 attgtaaaag acgaaaaaat gggaccagag ccaattatca aaagtctctg ggcggtaact
     4861 gtggttgcca ccatcgtaat cctgtttgtt gccctgtact ggtaaggagc ctgagatgat
     4921 taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg
     4981 tatgtggagc gcaattattg cgccggtgat gatcctgctg gtgggtattc tgctgccact
     5041 ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt
     5101 cattggtcgc gtattcctgt tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg
     5161 tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct gcgggcaaat gggttttcta
     5221 cggtctggct gctatcctga cagttgtcac gctgattggt atcgttacaa tctaacgtat
     5281 cgccaatgta aatccggccc gcctatggcg ggccgttttg tatggaaacc agaccttatg
     5341 ttcaaaacga cgctctgcgc cttattaatt accgcctctt gctccacatt tgctgcccct
     5401 caacaaatca acgatattgt gcatcgcaca attaccccgc ttatagagca acaaaagatc
     5461 cccggtatgg cggtggcggt aatttatcag ggtaaacctt attactttac ctggggctat
     5521 gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc
     5581 agcaaaacat ttacgggcgt gcttggtggc gacgctattg ctcgagggga aatcaagtta
     5641 agcgatccca caacaaaata ctggcctgaa cttaccgcta aacagtggaa tgggatcaca
     5701 ctattacatc tcgcaaccta cactgctggc ggcctgccat tgcaggtgcc ggatgaggtg
     5761 aaatcctcaa gcgacttgct gcgcttctat caaaactggc agcctgcatg ggcgccagga
     5821 acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg
     5881 tctggtttga gttttgagca ggcgatgcaa acacgtgtct tccagccact caaactcaac
     5941 catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa
     6001 ggcaaggcag ttcatgtttc gccaggggcg ttagatgctg aaacttatgg tgtgaagtcg
     6061 accattgaag atatggcctg ctgggtacga agcaatatga atccccgtga tatcaacgac
     6121 aaaacacttc agcaagggat acaactggca caatctcgct actggcaaac cggcgatatg
     6181 tatcagggcc tgggctggga aatgctggac tggccggtaa atcctgacag catcattaac
     6241 ggcagtggca ataaaattgc actggcagca caccccgtaa aagcgattac gcccccaact
     6301 cctgcagtac gcgcatcatg ggtacataaa acaggggcaa ccggcggatt tggtagctat
     6361 gtcgcgttta ttccagaaaa agagctgggt atagtgatgc tggctaacaa aaactatccc
     6421 aatccagcga gagtcgccgc cgcctggcag attctcaacg ctctacagta aaatttcatc
     6481 gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca cttagaaaaa
     6541 aaccagtaag gaaac
//
LOCUS       DAZKGS010000709         5366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.guided.238, whole genome shotgun sequence.
ACCESSION   DAZKGS010000709 DAZKGS010000000
VERSION     DAZKGS010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.guided.238"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..619
                     /locus_tag="ACHOQY_005685"
     CDS             <1..619
                     /locus_tag="ACHOQY_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP9681512.1"
                     /translation="GQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            616..1230
                     /gene="prfH"
                     /locus_tag="ACHOQY_005686"
     CDS             616..1230
                     /gene="prfH"
                     /locus_tag="ACHOQY_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP9681513.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1287..2744)
                     /gene="pepD"
                     /locus_tag="ACHOQY_005687"
     CDS             complement(1287..2744)
                     /gene="pepD"
                     /locus_tag="ACHOQY_005687"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9681514.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3005..3463
                     /gene="gpt"
                     /locus_tag="ACHOQY_005688"
     CDS             3005..3463
                     /gene="gpt"
                     /locus_tag="ACHOQY_005688"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9681515.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3555..4799
                     /gene="frsA"
                     /locus_tag="ACHOQY_005689"
     CDS             3555..4799
                     /gene="frsA"
                     /locus_tag="ACHOQY_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9681516.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4857..5258
                     /gene="crl"
                     /locus_tag="ACHOQY_005690"
     CDS             4857..5258
                     /gene="crl"
                     /locus_tag="ACHOQY_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9681517.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5297..>5366)
                     /locus_tag="ACHOQY_005691"
     CDS             complement(5297..>5366)
                     /locus_tag="ACHOQY_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9681518.1"
                     /translation="DSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1294 a         1316 c         1440 g         1316 t
ORIGIN      
        1 ggggcagtct attttacagc ggcatattgc ctcgttctcg catcatggtt tgcctcaagg
       61 cagtgacgac gcgctacggt atattgcgga acatgacgat gcgctggcgt ttgcgtgtat
      121 taatcgccag atgatcgctt tgcgcataat gcaacaagtt aaggccacgg gaagtccggt
      181 tctggatgtg gcgcataact ttgttagcgc gtgtcaaatc ggcgatcaac agggctggtt
      241 gcatcgtaaa ggtgccacgc cggatgacaa cggtctggtg attattcccg gttcacgtgg
      301 tgattactcc tggattgtac agcctgttgc gaacgaaaaa acgctccact cgctggcgca
      361 tggggcaggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag cgaaatacac
      421 ggcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg ataaacaact
      481 catctttgaa gaagcgccac aagcctataa atcggctgaa agcgtggtgc aatgtctggt
      541 gcaggccggt ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag
      601 tggagggaaa aaaggatgat cttgctacaa ctctcctcag cgcaggggcc ggaagaatgt
      661 tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg acaagacgtc
      721 gcggtaacgg tgctggaaac agaaacgggc cactactctg acacgctgcg ttcggcgctg
      781 atttctctgg atggcgataa cgcatgggca ttaagcgaaa gctggtgtgg cactattcag
      841 tggatttgtc cgagtccgta tcggcctcat catgggcgca aaaactggtt tctgggcatt
      901 gggcgtttta ccgctgatga gcaggaacaa tcggatgcaa tccgttatga gacgttgcgt
      961 tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg cgccacgcat
     1021 ttggcatccg gtattagcgt gaaggttcag tcagagcgta gtcagcatgc taacaagcgg
     1081 ctggcacgat tgctgattgc ctggaagctg gagcagcagc aacaggaaaa tagcgcggtg
     1141 ctgaaatcgc tgcggcgaat gttccatcac cagattgaac gtggcaaccc gagacggaca
     1201 tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa
     1261 acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc
     1321 agcagcgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg agagtgtgga
     1381 ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt tttgaacaga
     1441 ccacattcca ggcccgcgtg gataatctgg atgttcggtg ttttgttgaa cagacgctga
     1501 taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat
     1561 gcgcctttcg cttcggtttt cgcgccagct agtttaccca gcgaatccag catgctcacc
     1621 acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc tacgttattg
     1681 tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt tgccacgtcg
     1741 gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat
     1801 ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc cagatttttc
     1861 tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag agatttcagg
     1921 acgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta
     1981 cgcagtgtgc cgccgttgaa atcgataagg cgcagatcca gttcttccgc atgacccgcc
     2041 aggaagcgca ccagtagttt gttggcatta cccaggccaa cgtggatttc accgccggag
     2101 tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct
     2161 tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt
     2221 tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt
     2281 aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg
     2341 ccgtgaacca cgttttcgtc agccagaacc gccagcgcag acgccatacc aatgccgtta
     2401 tctgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata
     2461 ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg
     2521 gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttt acgaatcagg
     2581 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
     2641 tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa
     2701 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
     2761 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
     2821 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     2881 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     2941 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     3001 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     3061 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     3121 tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     3181 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     3241 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgctgttgc
     3301 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     3361 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg aacagccgtg
     3421 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     3481 actgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca gtaagtattc
     3541 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     3601 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     3661 agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     3721 ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt attgtgatga
     3781 gcgatgctga acgtaccgac gatgatttat acgatactgt gattggctac cgtggtggca
     3841 actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc tgtgcggaag
     3901 aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac aacattgccg
     3961 cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta tcaaaccgcg
     4021 cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag tttaccgtac
     4081 ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc ccgttcccga
     4141 cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc ctgtatgaac
     4201 gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccatcg gtgggctttt
     4261 cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc ttaaaggcgc
     4321 tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc cgtttcggcg
     4381 ctaacgttgc cgtgcgtctg gcatatcttg aatcgccgcg tctgaaagcg gttgcctgtc
     4441 ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag gtgccggaaa
     4501 tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgat gacgcgttac
     4561 gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt cgctgcccaa
     4621 cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag gactcacgct
     4681 taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac ccggtgtatc
     4741 ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc ttgtgttaaa
     4801 aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag atagcaatga
     4861 cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca ctaggcccgt
     4921 atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg gctgtatgcg
     4981 tcaacgtgaa accggcaccg gaagtgcgtg agttctgggg ctggtggatg gagcttgaag
     5041 cgcaggaatc ccgttttacc tacagttacc agtttggtct gttcgataaa gcaggcgact
     5101 ggaagagtgt tccggtaaaa gacactgaag tggttgaacg actggagcac accctgcgtg
     5161 agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg gaaccggcgg
     5221 atgattttcg tgacgagccg gtgaagttaa cggcgtgagt gaaatgtgcc ggatgcatca
     5281 catccggcaa taatcattaa aactgatagg tcatgccaac cgcgacaata tcatcattat
     5341 taatattcaa tttgttatcg ctatcc
//
LOCUS       DAZKGS010000710         1737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2109W22893
            SAMN43267657-rid23082383.guided.383, whole genome shotgun sequence.
ACCESSION   DAZKGS010000710 DAZKGS010000000
VERSION     DAZKGS010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267657
            Sequence Read Archive: SRR30307522
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350840.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350840.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:46:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,693
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,347
            CDSs (with protein)               :: 5,347
            Genes (RNA)                       :: 92
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 113 of 254
            Pseudo Genes (incomplete)         :: 137 of 254
            Pseudo Genes (internal stop)      :: 59 of 254
            Pseudo Genes (multiple problems)  :: 46 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267657-rid23082383.guided.383"
                     /serotype="O 26:H11"
                     /isolate="2109W22893"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-09"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHOQY_005692"
     CDS             263..1210
                     /gene="stxA1a"
                     /locus_tag="ACHOQY_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9681519.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHOQY_005693"
     CDS             1220..1489
                     /gene="stxB1a"
                     /locus_tag="ACHOQY_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9681520.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          462 a          304 c          428 g          543 t
ORIGIN      
        1 gagtttgcca gcctccccca gtggctggct tttttatgtc cgtaacatcc tgtgtatcaa
       61 taaatgttgt tatctacgta cgtcaagtag tcgcatgaga tctgaccaga tatgttaagg
      121 ttgcagctct ctttgaatat gattatcatt ttcattacgt tattgttacg tttatccggt
      181 gcgccgtaaa acgccgtcct tcagggcgtg gaggatgtca agaatatagt tatcgtatgg
      241 tgctcaagga gtattgtgta atatgaaaat aattattttt agagtgctaa cttttttctt
      301 tgttatcttt tcagttaatg tggtggcgaa ggaatttacc ttagacttct cgactgcaaa
      361 gacgtatgta gattcgctga atgtcattcg ctctgcaata ggtactccat tacagactat
      421 ttcatcagga ggtacgtctt tactgatgat tgatagtggc tcaggggata atttgtttgc
      481 agttgatgtc agagggatag atccagagga agggcggttt aataatctac ggcttattgt
      541 tgaacgaaat aatttatatg tgacaggatt tgttaacagg acaaataatg ttttttatcg
      601 ctttgctgat ttttcacatg ttacctttcc aggtacaaca gcggttacat tgtctggtga
      661 cagtagctat accacgttac agcgtgttgc agggatcagt cgtacgggga tgcagataaa
      721 tcgccattcg ttgactactt cttatctgga tttaatgtcg catagtggaa cctcactgac
      781 gcagtctgtg gcaagagcga tgttacggtt tgttactgtg acagctgaag ctttacgttt
      841 tcggcaaata cagaggggat ttcgtacaac actggatgat ctcagtgggc gttcttatgt
      901 aatgactgct gaagatgttg atcttacatt gaactgggga aggttgagta gcgtcctgcc
      961 tgactatcat ggacaagact ctgttcgtgt aggaagaatt tcttttggaa gcattaatgc
     1021 aattctggga agcgtggcat taatactgaa ttgtcatcat catgcatcgc gagttgccag
     1081 aatggcatct gatgagtttc cttctatgtg tccggcagat ggaagagtcc gtgggattac
     1141 gcacaataaa atattgtggg attcatccac tctgggggca attctgatgc gcagaactat
     1201 tagcagttga gggggtaaaa tgaaaaaaac attattaata gctgcatcgc tttcattttt
     1261 ttcagcaagt gcgctggcga cgcctgattg tgtaactgga aaggtggagt atacaaaata
     1321 taatgatgac gataccttta cagttaaagt gggtgataaa gaattattta ccaacagatg
     1381 gaatcttcag tctcttcttc tcagtgcgca aattacgggg atgactgtaa ccattaaaac
     1441 taatgcctgt cataatggag ggggattcag cgaagttatt tttcgttgac tcagaatagc
     1501 tcagtgaaaa tagcaggcgg agattcataa atgttaaata catctcaatt cagtcagttg
     1561 ttgccggtct gataatagat gtgttagaaa atttctgcat ggtgaatccc cctgtgcgga
     1621 ggggcgactg gtgaacggta tgatctcttt gatgatcgta agcgagaata cgcgggtttg
     1681 gtggcaccag gccgaactca ccgggaggca cccggcatca tgctgtatac agagatt
//
